LOCUS       DBNCWK010000001        84943 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.001, whole genome shotgun sequence.
ACCESSION   DBNCWK010000001 DBNCWK010000000
VERSION     DBNCWK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 84943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84943
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.001"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..1467)
                     /locus_tag="AC06IO_000001"
     CDS             complement(<1..1467)
                     /locus_tag="AC06IO_000001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HIO9873226.1"
                     /translation="MNYLPRRIVYINEKGHEEQCSDNEFFAINAPLIILGEPGAGKSE
                     LVKNFAKSSGVTVYNASSIDLFPEIKSVNPPAKIIIDGIDEITAYEPGVSLSRRILSK
                     LANHDFPNFILTCRAADWQGTVNIKTIQDRWCTKPIIGHLLPLNDQEIIEFANIKTGG
                     QNGREFLLEAKKRDFVELLRNPQNLTLFLKAIQQNDGWPNSRLELYEKSCSTLLKEHN
                     QTHTSIKVTSNCTKLEDAAGFICAQLLLSGKSGICIESSENVLAPGLAEFTNESYCET
                     TAREVLSTMLFRSVGNNIIEPCHRTVAEFMAAKWLSNALIDKISIKRLEGVLYCGNTI
                     VPTALRGLHAWLATLHQPSRDMFIKRDPYGFLRYGDPKTLTLQQSKNLLTYLEKVAEE
                     DPYFKSDDWYVSLGNGLLKPELRDDILRIIKSSSTTFQLSYLLLESIELIESNDFVDS
                     IKNELLPVLLNKSVTVEERKLIVNALKKCSVEPDWINIS"
     gene            complement(1646..1722)
                     /locus_tag="AC06IO_000002"
     tRNA            complement(1646..1722)
                     /locus_tag="AC06IO_000002"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1686..1688),aa:Pro,seq:ggg)
     gene            complement(1836..2213)
                     /locus_tag="AC06IO_000003"
     CDS             complement(1836..2213)
                     /locus_tag="AC06IO_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIO9873227.1"
                     /translation="MTAPEITLSRVYSPPEHKEGLWILVDRLWPRGIKKDSIPLDMWL
                     KDIAPSPSLRKWFHQDREHWPEFAKRYARELKEKPELIEHVLALSSTSPVTLFYAAKD
                     LQHNHALVLQKVLKSWPKIPEIE"
     gene            complement(2518..3276)
                     /locus_tag="AC06IO_000004"
     CDS             complement(2518..3276)
                     /locus_tag="AC06IO_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873228.1"
                     /translation="MLVTPRFKIMVVAKKREETQKDERARAIENNPCSICRSLGLPFC
                     RGHGGGSGGGGSDSASDDKLEGNNHQLLAPKDPANVPSIDQLLLHSGLWHLPEDADFV
                     FEFKDPLALVNMTLNMELGFLVLQGKRELSNEEQKALDELFDTIKQEFNLFKNSIKDP
                     QVPPETMTLAREGNSLTIKIPSPKYYDLFVQQLMDKNLLPGNAPVLQHQNQSVKHPEK
                     LGAVMEKDSSREEYKSSLPNPFDISRGPKLPDWV"
     gene            complement(3372..3626)
                     /locus_tag="AC06IO_000005"
     CDS             complement(3372..3626)
                     /locus_tag="AC06IO_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873229.1"
                     /translation="MIELEPEPDTVLDPKDILTEEMKLEGSDNDMDHNQFTRGDEVDL
                     VGDGVPGYETTGQDGTDDEIEEQREARYTPTLNPNPKNVP"
     gene            complement(3828..4241)
                     /locus_tag="AC06IO_000006"
     CDS             complement(3828..4241)
                     /locus_tag="AC06IO_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873230.1"
                     /translation="MDIRCKTVLYKQLQEFNMINMKLKATLIACLSVLTLSSYANSSE
                     NKEAILQQCHDLASTVASLVSSQAKKTCAEKLVIASIHIDSAADWIVEDVHSAAKQEL
                     DNAIYSLQYAELNSCNRYIQISHSKLEAQRIKSLL"
     gene            complement(4437..5594)
                     /locus_tag="AC06IO_000007"
     CDS             complement(4437..5594)
                     /locus_tag="AC06IO_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIO9873231.1"
                     /translation="MDDLLFLDEQLHDDERMIRDSVSRFVSNDVIPLMAESYEHAHFP
                     KELIKKSADLGLLGLTLPAEYGGAEASYVAYGLVCQELERGDSGLRSFVSVQSSLCMY
                     PIFKFGSEEQKKRYLPEMAAGNIIGCFGLTEPDSGSDPASMRTHAKKIEGGWILNGAK
                     MWITNAPIADIAIVWAKTEAGIRGFIVDTKSEGMSCPEIKLKMSLRASITGELVFDNV
                     FVPDENLLPKSEKGLGAALSCLSQARYGIAWGAMGAAMACFDIARDYLLERKQFAKPL
                     ASFQLIQKDLADMYTEIIKAQCLNLQIGRLKDQHRETPTMISLAKGNACREALKIARK
                     CRNLMGGNGISLEYHVIRHMLNLESVFTYEGTDNVHTLVLGRHITGINAFG"
     gene            complement(5572..7809)
                     /locus_tag="AC06IO_000008"
     CDS             complement(5572..7809)
                     /locus_tag="AC06IO_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HIO9873232.1"
                     /translation="MLDRASVVDEQFLKRVLQGDFPQLRSSMSPDTAELDKKTAIEFF
                     DSQIKSRLLDIIARQLKEKGLSFYTIGSSGHEGNAVLGQVFRASDMAFLHYRSGAFFL
                     QRAKQLPGIDGVKDILLSLVAAASDPISGGRHKVFGSVPLNIPPQTSTIASHLPKALG
                     AALSITRARELGLPSKLTPDSVILCSFGDASTNHATSQATLNACSWITQQNYPLPIVF
                     ICEDNGIGISVPTPSSWIKSSVSSRPGIHYIECDGLNIADVFAKAQEADYLARVKKQP
                     VFLHMRCVRLLGHAGSDIESQYNTQEEIEQREANDPLLHTAGILYREGWMSLQAMVEL
                     YQDNRALIEAKALEAIREPRMSSAEEIMASIVPRIDKKQKYSLPDDNRRAQVFAGAYN
                     QLTLKRNLCQQINFALTDLMMQYPNMLIFGEDVGKKGGVYRVTADLQARFGQRRVFDT
                     LLDETTIIGTAIGLAHNGFIPVPEIQFLAYLHNAEDQLRGEASTLSFFSSGQYQNPMV
                     LRIASLAYQKGFGGHFHNDNSIAVLRDLPGVIVACPSNGPDAAKMLRTCMRLAYEEGR
                     VVVFLEPIALYMTKDLYSPGDNGWLFEYPSPDEMISQGEVGVYGEGDTVILTYANGYY
                     LSRQAEKVLREAHNISVKIIDLRWLSPLPKDAILKEIAKAKRILIVDEGRQSGSISEG
                     LMTLLMEEASPRLKIKRITGKDCFIPLGTAWQYLLPSQESIIDAVIALQSDKREKESG
                     RLVVS"
     gene            8047..9213
                     /locus_tag="AC06IO_000009"
     CDS             8047..9213
                     /locus_tag="AC06IO_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HIO9873233.1"
                     /translation="MSVEMSKTSSVRFPPGQEIKLAKPATELYVGQILKTVVVTTLSN
                     DQVLININGQNLNAKASHHFTPGELFEVKVLANHPETILEVQQKTPLPSLLQNALLQY
                     LPKQAPATNLLQTLTQLMAENKLSAPINQVMKTILGNVPVVTQLPNQLMQAISQSGVF
                     LESKLLKGKIPREIQSDFKGLCFKLLHCLGTDSKLPINLPNELESGFLQQAPLPLPGA
                     IPQPLTKDTILNLIGLSTEAVHHILREQVTQALARITTNQINYLSQENQETYLIMLDF
                     PLKTPDGIDVIPIMIKQHKAEPMQLSKWSISFALNLSHLGSIQATVSIYAKNIDIKVN
                     TQQSSTLQLINDYHKDLEETLYESGLHLREWKLQVGLEENHIDATNLRLLDIKI"
     gene            9210..9476
                     /locus_tag="AC06IO_000010"
     CDS             9210..9476
                     /locus_tag="AC06IO_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HIO9873234.1"
                     /translation="MKRKKPVAVALRYDGNSPPKVTAKGEGLFAQQIINTAKEHGIPL
                     EQNPELTALLSQVHLNEEIPKALYIAVAQILAFLYYINGKHSKE"
     gene            9599..9778
                     /locus_tag="AC06IO_000011"
     CDS             9599..9778
                     /locus_tag="AC06IO_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873235.1"
                     /translation="MKWIMLIVVIITTAGCCCTTAEVVEYQQVTVAAPVVEPVILDVD
                     DPEPLDVTTTTIEFY"
     gene            9960..12698
                     /gene="sidF"
                     /locus_tag="AC06IO_000012"
     CDS             9960..12698
                     /gene="sidF"
                     /locus_tag="AC06IO_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PI phosphatase SidF"
                     /protein_id="HIO9873236.1"
                     /translation="MPRITENIETYVTHLLSDLEPVPSQNQHLAYGYPGERQVFKDPI
                     LDGKQVVVVNSQYDKHGRPVTGQPDVIQEANNYIDNLVAAAKSLIDKDKKGEKDLRKN
                     AIDEMAKTFKKSISETIPSDKAKADLFSSKKSSANKDFLEQLAKVEGSLQQFTQAVAK
                     ASGHKLKALDKEGHNIRSHNRDTLIHRFKAPNSKEGEEQFIMYIPCGRYTKRQQRLMG
                     KDESEVNRDHDTTSHKRSDLVLGNSSMARMIAGTRHSDGTVTIHHDSFSGPGARMPYS
                     DFKGADDYKKLAIKAVTLINQEEVIQTLAQRQIDRMTNEDLWNKIPEEYRPDELPPDA
                     EKARAQLIKLYVEHNPLSVTECYTQVVTAGQRVAAENQKEQFEYVRQMMDAFDGSKAK
                     ITIQTGSNTEVETEVGYQARMSSWGVNWFRQVGALNPLSDNSVTKNQNARFVNQMTDD
                     VISNLGKVAQNLGDYDKAGALHTLLKGPNVSDLNQQITEKENALKEVKGAYREALFSY
                     FEEYQKGEGKWDQAKLDQLKNQVDGYEKSIKKQESAIYELHNQIDALRKAYYTEHKGQ
                     INKALQELKEQISPVIQNKETDPETKSRLQHFYNSCAYLMQAQELYYENTWHHGKNNF
                     KLQTLMASLSCELDYANTKGCKSNNDRGQRLAQKIVGNALWTAMSEDGLFTGEFLDHR
                     RTHGKEVSKVEQLDRELTTIQALHHTANTGVSGGKFEIQDKANFADNGLFGKVANFAK
                     VKEMGPENAFTKNMGTKIKAGLGLGLLAAGGLVALTLLFPPAGLAVAALGIGVAAGAA
                     IVAGVTFAATYLFGTAIDAINNKIEEGKIKSIVREGMKVASENSPQHKTEQKDHLTVN
                     AKLHQQLGVTRESVLERQSTPKQEFEKPKDMTSKIDLTPAMEDDKRSESTPTPVNF"
     gene            12724..13455
                     /locus_tag="AC06IO_000013"
     CDS             12724..13455
                     /locus_tag="AC06IO_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIO9873237.1"
                     /translation="MKKISVLIFLYLISTTSFSLPKGFVYLHDVAPDIIEDIRYATHN
                     NFIGNPIPGYKRGVCIVTRQTAQQLKKAEEYIKPKGYTLKVYDCYRPQRAVDYFYKWS
                     RNAKDNRMKPEFYPREIKQDLFDRGYIALTSGHTRGSTLDLTLVKLTASQEKKSNHPL
                     TRCYDKKPDYLDDDSIDTGTRFDCLDVSANIDYSRLNKHQKANRELLQKLMKRFGFRP
                     YYYEWWHFTLKNEPYPDTYFNFPVE"
     gene            13657..14751
                     /locus_tag="AC06IO_000014"
     CDS             13657..14751
                     /locus_tag="AC06IO_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIO9873238.1"
                     /translation="MRVSKLLFLTLALSGNLFAQDIKVVGTLNQTLKAPQNKTLNTKP
                     AIQQIKLLKMQLSNSAIKALARKTDITLKKPNTITTSTALPSKVELGMNNVPVLNQGS
                     FGTCVTFATTAAIDAALNKGDYISQLCQLQLGLYLEKNGHSPSGWDGSLGRIVLSQME
                     NFGIVSKEKQKNLGCGGLTEYPDNEQPTPDHAMSLEQFHEMSEDPETNPVIYTPILDV
                     YQAVLDRTDTNKTINDIKTALNQKDRVTFAVLLLDFDLGVMGAVGTNKTQFDTWVLTP
                     EIARDIYLRPFFGGHEMIITGYDDNAIAVDDNGREHKGLFTLRNSWGEQFGDKGNFYM
                     SYDYFKVLTIEAQRIRALADNESEEESMTA"
     gene            14972..16522
                     /gene="plaD"
                     /locus_tag="AC06IO_000015"
     CDS             14972..16522
                     /gene="plaD"
                     /locus_tag="AC06IO_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family phospholipase PlaD"
                     /protein_id="HIO9873239.1"
                     /translation="MAQKPTKITHLVVLGDSLSDRGTLNKRELLGFIPMSYLSGLRSK
                     SPKGRFTNGFLWGDYVGATTAEQFEIEHIRRKLRLHNNARDNADIGDELLINDKARRE
                     NENSFSLNEDNHILFKGERFARFYCEGGLTSHDYSCSFTFNLVLFFTRLILATLGGKR
                     TQLLADDKKYNISNLEKSETLVVEWSGANDLITANDKPSRAAAEKAVNDRMQNIEALI
                     HNRYRNFVLFNLPNLSLTPRFQRKNAEEQRNASDCTEYFNQQLQAKSLELINKYKELG
                     IPVNLSVFDVNEQFKEVYSNPEKYGFDRDKLKSPYVESDLFKENQKNPIDQKEHISPG
                     RGYMFWDDVHPTTKMHDWLAERFKEKYYDKMFRFEPPLQAKHRHKETADTLAKTTDSM
                     VKTDKPVKKLPENIVLMLNAIHSRAQLMCQSTDVQRRKKGELLKHFIFEIKCQHGNLE
                     NIYGLISAFTLNPDHSKIIKTHQNPIIDFFRNKVTTRSEDDIAALQKAVATYLQPKES
                     NEHLVKLT"
     gene            complement(16539..17696)
                     /gene="legS1"
                     /locus_tag="AC06IO_000016"
     CDS             complement(16539..17696)
                     /gene="legS1"
                     /locus_tag="AC06IO_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegS1"
                     /protein_id="HIO9873240.1"
                     /translation="MDFIIRVVLLLTGLVSLAFSAPKFLTISDIHYGAENIAKDGQDT
                     GKEFLDVTFKRFEELSQKVDFILHLGDLPTHSLFSTSKKEEYEQVVFHGLYEANPGQK
                     PMFYITGNNDSLLGNYQPFSSDNKSPLNMALDWDGACAHCDGLIIDDTHMRKDGYYSS
                     YVIPNNKEIILIALNSVQWTKTPVFLPKYPNQQRDAFVQLFWLEQQLKNHRAKQLLLA
                     MHVPPGTAYNGNRFWHDIYLDRFLTLLDKYHQSYDQITILFSHSHMEEFRKVKLSDGS
                     TIYAFSTPGISRAHHNNPGMKVFDLDKQMRVRNFTTYFTSDLYEWGNEHYQAMNTPDA
                     IFPNCQNKTIPQCLDTLSPEQVCNNLEAGLFYGVKSTRVPYQGCIKTYQIN"
     gene            complement(18656..20449)
                     /gene="dacB"
                     /locus_tag="AC06IO_000017"
     CDS             complement(18656..20449)
                     /gene="dacB"
                     /locus_tag="AC06IO_000017"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIO9873241.1"
                     /translation="MKRTLTGAFMWAVWSLSSHAASMQFEVDKLINRLNPRVNLGIVV
                     TDLTSGETLYKRNANRLYIPASNMKLFSEAAALMALGPDYQFKNQLSTSANQLQQGVL
                     HGNLYLHLSGDPSFSREDLKTLLSSLKDWNITTIQGNVIIDSSLMSIPAYPPGWLTSD
                     LSYSYGAPIAPLMVDSNRLTITVNPGAKAGAPAIVEVDDGGGTINLNNQATTKASEKG
                     CGVGFYLDPENNLTVRGCVGLGQWAVQQRIAIKNPFVYAQGMIVSELAKSNIKLNGQV
                     LLGRAPAGTLLIATRYSKPISQLMADTLKPSDNLYADSLYLHAAAKIKGSPVDWKQAQ
                     PVIKNFLQQQTGIDLKDSNFTDGSGLSRYNLVTPAQTMALLKFLYQRFPLSYEYIAAL
                     PISGRDGTLQKRFKTPNQQGFVRAKTGTMTGMNSLSGYLYTANGHTLAFAMYINRLPG
                     KPAGPGRPLLDALCTYFLQQSPTSSRLARVLSPHSRIKFQFNPTQIELQRVHQAKWRR
                     LETAVRQVLRGQDVNVVYRGNELIVTDNQSNANSVWKALQSIGKKYSFAVALSSKVMP
                     VTPSGKPLLLWVQAPLSENTAERTWIIREAV"
     gene            complement(20541..21332)
                     /locus_tag="AC06IO_000018"
     CDS             complement(20541..21332)
                     /locus_tag="AC06IO_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIO9873242.1"
                     /translation="MQTRFTYLPVTSLQAGQYQPRQDFNVTELQELAQSISSQGLIEP
                     LVVRPIAKERYEIIAGERRWRAAKLAGLNEVPCLIGDYSDQQACALTLIENIQRQDLN
                     LIEEATGYKRLIEEFHYHQDEIAALVGKSRSHVANILRLLTLTDKVKQLVRDKVLSFG
                     HARVLIGLNPEQQEMFAHQVIGEEWSVRHLEQEIKSYKNKDTNSPKNPRKDRDIERLQ
                     TILAEQVGAPVQIVNDGEDGGWLKVKFFDNDTLAGLLERLGLRYD"
     gene            complement(21449..21952)
                     /gene="ceg33"
                     /locus_tag="AC06IO_000019"
     CDS             complement(21449..21952)
                     /gene="ceg33"
                     /locus_tag="AC06IO_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg33"
                     /protein_id="HIO9873243.1"
                     /translation="MAAQLDPSSEFAALVKQLQREPDNPGLKQAVVKRLPEMQVLAKT
                     NSLALFRLAQVYSPSSSQHKQMILQSAAQGCTNAMLSACEILLKSGAANDLITAAHYM
                     RLIQSSKDSYIIGLGKKLLEKYPGFAEELKSKSKEVPYQSTLRFFGVQSESNKENEEK
                     IINRPTV"
     gene            complement(22126..22875)
                     /locus_tag="AC06IO_000020"
     CDS             complement(22126..22875)
                     /locus_tag="AC06IO_000020"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIO9873244.1"
                     /translation="MIIDWPVIIVLFCLSIPGVLIVMHRLVYFLLADNTERLKKRFSR
                     FVVLQTLFMTFVMSFAGSILSSRTGLGDPLLEGLLQGKASLDSFQVILLPTFLYALLG
                     LVIFCIMYYGLFGSILDEKSFKIMSELRSKIGLDGCVLYGGVVEEVIARWGLMNVVCF
                     FALLFTSQINSEIIIISIFLSAFIFAVGQLPAYIAAGCTPSRRFLYSFVLLSLSQSIL
                     FGFLFWQYGLTSAILAHMLFHLGWDRYEKLS"
     gene            complement(22872..24155)
                     /gene="rsmB"
                     /locus_tag="AC06IO_000021"
     CDS             complement(22872..24155)
                     /gene="rsmB"
                     /locus_tag="AC06IO_000021"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIO9873245.1"
                     /translation="MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFC
                     RHYFRLQSIADTLINKRPKDNEVWIVLLIGIYQLQYMNKPDYAVVKETVSLLGQVKKN
                     WAKGLVNAVLRNFCRRQDHILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAEAND
                     RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGS
                     VSVQDAAPQLAAYLLSLKPGQRVLDACCAPGGKTCHILEKESGLASCFALDLDPQRLQ
                     RVRDNLNRLDLQATVVEGDVLEPRSWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRT
                     DEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIARFVAEHSDCQVSD
                     GKWSWGRWTGHGQQILPGEHGMDGFFYSVLRKDEK"
     gene            complement(24152..25096)
                     /gene="fmt"
                     /locus_tag="AC06IO_000022"
     CDS             complement(24152..25096)
                     /gene="fmt"
                     /locus_tag="AC06IO_000022"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIO9873246.1"
                     /translation="MNGLTVVFAGTPEFGLPCLDALIQSRHHLKAVYTQPDRPAGRGR
                     KLQESPVKEWAINHQISVYQPLNFKNQEAVDELSALKPDVMVVIAYGLILPKAVLEIP
                     RLGCINVHASLLPRWRGASPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKAACPVTS
                     SDTAGSLHDKLAKMSVKPLLDVLEALASNSAQFELQNNELATYAGKINKEEARINWHQ
                     SAVEIDRKIRAFNPWPVAYTLVGELMLRIHQAKATDIMSTEMPGMVLNIDKTGMLVAT
                     SDNALLVEKIQFPGAKVISVRDWLNSGKTQLHTGLMLQ"
     gene            complement(25093..25605)
                     /gene="def"
                     /locus_tag="AC06IO_000023"
     CDS             complement(25093..25605)
                     /gene="def"
                     /locus_tag="AC06IO_000023"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIO9873247.1"
                     /translation="MAIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARG
                     VGLAAPQIGVSLRLSVIDIVGDKKEQIVIVNPEIVSSHGEKEFEEGCLSVPGAYDTVV
                     RAEKVTVKALDRFGKPFEITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLD
                     KFKRLQARKP"
     gene            25699..26736
                     /locus_tag="AC06IO_000024"
     CDS             25699..26736
                     /locus_tag="AC06IO_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIO9873248.1"
                     /translation="MRYCLLIFCFIISSITHALSLRPDSPSRYVVQPGDTLWSISSRF
                     LNNPWEWKALWRANPQIQNPDRLYPGAVLALEYYQNIPYLRVLSNGTIKLSPNIRVTP
                     QEDAVPPIPLGDIKPFLDESLILDVNVLNRAPYVVALMSERMLGGQGDEIYVRGLHPS
                     KEMPQGGTIGYSIFRGGRNYFDPITHELLGYKAVLVGYGELVAGGEPATVLLTSINQG
                     IKINDKVLINNHPEFELYFEPETPARQVRGYIIEMPDNMPVGNTQEAVGGVIIINLGE
                     TSGIKPGDVLAIYGKERIVNDPQNHLRPITLPQERLGEAMVFRVFTKASYALIVRSTR
                     AIHLLDTVTNP"
     gene            26733..27818
                     /gene="dprA"
                     /locus_tag="AC06IO_000025"
     CDS             26733..27818
                     /gene="dprA"
                     /locus_tag="AC06IO_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIO9873249.1"
                     /translation="MNNLQPLLALNRMKKIGPRTVLKLQKRWPDLNLMFQLSSVDLEE
                     AGLPSWLAQTIKNFDPDFIKTDLDWLSSSENHHILTWDSPYYPALLKEIADPPFILYA
                     KGKLSALTQPNLAIVGSRNASVTGNENARAFSREISRHGVSIVSGLALGIDAHAHLGC
                     LDEGGKTIAVLGTGIDCIYPRRHLKLAEQITENGLLLSEFPLKSPPIAGHFPLRNRII
                     SGLSLCILVIEAAIKSGSLITARMALEQNRDVLAIPGSIHNPLARGCHYLLQQGAKLV
                     TSVADVLDELKIEHHQFTSNKPIFSLASGKENLVKFIGFETTTIDQIIDRSGYGMEQV
                     TSGLAELELKGAVMAVPGGYIRCEYER"
     gene            27808..28245
                     /locus_tag="AC06IO_000026"
     CDS             27808..28245
                     /locus_tag="AC06IO_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HIO9873250.1"
                     /translation="MKDNLFEMLLNLFEKSLTQLQKSHKSNDQDSQSQLLEEDLLSAE
                     EQSLFIKSAQDKSTRIFTYDEQMKLTKASYQFLMRMKLWNILDIDQFESIINQLQFSE
                     SRIVTLQETKWTIRNALASTLNEEQLTFLDLVLYQTEDELTIH"
     gene            28324..30603
                     /gene="topA"
                     /locus_tag="AC06IO_000027"
     CDS             28324..30603
                     /gene="topA"
                     /locus_tag="AC06IO_000027"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIO9873251.1"
                     /translation="MSKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSV
                     NPEKHFSMTYVPIEKNARHIDTIAKTLKKSDSLLLATDPDREGEAISWHIFELMKERN
                     LLKDKSVHRIFFNEITKAAIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLIVEREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEE
                     KLQQFSVTQQEQAHEIKKQLIAQAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARK
                     LGFTARKTMMVAQQLYEGIDIGTGTVGLITYMRTDSVNLAKEAIDEIRDYITQRYKGD
                     NCPNSPRIYKTKSKNAQEAHEAIRPTSIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQ
                     MADAILDTVSVDFSCGKGNTFRANGSTIAFPGFLSVYEEGRDDSKDEDNEDKILPAFN
                     VGEKIKVSDIETNQHFTEPPPRYSEATLVKALEEYDIGRPSTYASIIHTLQQREYVVV
                     EKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTAGLEDTLDAIARGEKDWIPVLEEFWQP
                     FVQQIQNIDEQVQRKDVTTELLDEKCPKCQKPLSIRLGKRGRFIGCTGYPDCDYTQDI
                     SNPEGEKSEPEVVEGRSCPLCHGALHIKTGRYGKFIGCSNYPECKHMEPLEKPSDTGV
                     TCPKCSQAKILQRKSRKGKIFYSCGNYPKCDYALWNEPVDLPCPKCKWPILTLKESKK
                     FGRQILCPREGCDYSAKED"
     gene            complement(30704..32677)
                     /locus_tag="AC06IO_000028"
     CDS             complement(30704..32677)
                     /locus_tag="AC06IO_000028"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIO9873252.1"
                     /translation="MNYRSDIDGLRAIAILFVLFFHGGLTLFPSGFIGVDVFFVISGF
                     LITSIIEKSLQNNHFSFMDFYSRRLWRLQPVFICLIVVTTLITLFYYLPDDLMLFSKS
                     ARKTSIFTSNVFFKNVTSDYFSPDSNQLPLLHMWSLSVEWQCYFILPVGIYLLYRLIG
                     PQHRIKIIYLLTITFFALSMYYSWSNPAKTYYQFSSRIFEFLIGSCVASASGSKRFLA
                     NKYVLNFITVLAVLTLFYIAMHHDINIGFPNWYTLILCLATAILIAAGQQEPQPIVTY
                     ALSLRPLVFIGLISYSLYIWHWPVLALMRYLNIEESNFVLLVTFGLISIIAYLSWRFI
                     EKPARKLHRIRFSYTVVCLLIMPVFVTHLSDYLIKKNAGYPHRFNEAIQVYAELNKYI
                     NPQRPLCLERKNTEVNHQCVLGAKNSDSRTGFMIGDSYSNQHWGFMDTLAKEANVSIL
                     AHATVACLALPDIYQFDWNVKNKIYQECHDQTNRYFNMIKTNHYDYVILGQNWNGYFG
                     DRIINQLNDNRSQELSLKRIEKALDKALELITTSGAKPVLIKSIALSKGNPYNCFFEH
                     IKLHKKYNPERCDFNLDVSEQIWFDNLFAKMQRKYKTLVIIDPRVAQCPSGMCKVDIN
                     GIPVFRDSGHITDYASYHLAKLYLQKNNNPLVS"
     gene            complement(32813..33253)
                     /locus_tag="AC06IO_000029"
     CDS             complement(32813..33253)
                     /locus_tag="AC06IO_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIO9873253.1"
                     /translation="MAENTTESNVIYFTRLHWIIFFGPFVGLGLVMAIYIYIIQLREV
                     SLFLGFFALIWILITWVTYHFSSITIKRKQVILRTGIIVRQTVDIPLNKIETIDIRQS
                     ILGSILSYGTLIITGTGGTRYLINYLYKPLTCRRYIEQLMNEPH"
     gene            33384..33806
                     /locus_tag="AC06IO_000030"
     CDS             33384..33806
                     /locus_tag="AC06IO_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIO9873254.1"
                     /translation="MKSISQCLNKQLSTICQHSMQLEELSLKISAFLPSHFISKCQVG
                     SFNKGCLVLTTTNAAFASEIRYLLPELRDKLRKEAGLYQLSSIKITIIAPSSQIENSA
                     QKKSLTLSEKAKATIISESQHCTYEPLKKALLHLAQSE"
     gene            33824..35128
                     /gene="lem28"
                     /locus_tag="AC06IO_000031"
     CDS             33824..35128
                     /gene="lem28"
                     /locus_tag="AC06IO_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem28"
                     /protein_id="HIO9873255.1"
                     /translation="MYSEFFFTIMLKYSTNMLELAMPKINKIVNGTDLTPHYLSEPNK
                     EFKIYRHNNEVYAVRFDNDEPMDYVLMWKSHKSEHTQNSEIIKNTEEDYKELGKGEQG
                     TVYEKTEDKAMKVSRGKHPREFYEEINLHIIEQQFFLKYHGIQEHFVLGLWNIKNEEN
                     VYFYMPKINAIPINKKIDQPKIEEFVLALKELNDAGYWHPDLANNPYHISPQNLIATE
                     EMVKTIDLDGGFRYDKGRVDELSRKSLVYGKDQWLYVYNFIYPPTDEEDNRIDWRVPV
                     EKWYENNRDESLSDNPHTLLRFYHEGLISLPKKLVHDLHETILEELSQDKKFKKHSHE
                     VGVNRFFSPKKQEIYEGLKGDGLKKVILKELRDSLAEIRTMEQLEEKKLEFLASPEMQ
                     ILAEGQDKTTKALNLKTSSRKKVMVIFKEAEERILNSPNVSI"
     gene            35428..36225
                     /locus_tag="AC06IO_000032"
     CDS             35428..36225
                     /locus_tag="AC06IO_000032"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9873256.1"
                     /translation="MLKQIVLTGLVIIGIVITLMYLFQRHLIYFPNRHTPKLEDYNAS
                     DMKVVSLRTKDNLHLKSWYKPASKHRPTILYLHGNAGHIGYRMPLVREFIDAGLGVFL
                     LEYRGYGGNPGKPGEKGLYEDGEAAIEFLIQHGVPSKRVILYGESIGTGVATHLATKY
                     LVCAVILQSPFTSLTRLAQYHYPLNFLKPWDQYNSLARMKKINAPILVLHGKLDQIVP
                     YQEGLNVFNEANEPKKMISFDDKEHNDLWSADNFSREIIQFALNQCS"
     gene            complement(36281..36697)
                     /locus_tag="AC06IO_000033"
     CDS             complement(36281..36697)
                     /locus_tag="AC06IO_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873257.1"
                     /translation="MSFSLSELLHYKNEKIVTHFCYSHPEYSIQEGLFLFEDLLAWMW
                     LNKQRRLRGKKTYLFGPLLILDEMWHSFILHTRDYIDFSMKYFGEYFHHDVEPVGFEH
                     VLDEEELADYLQDCFQYLNKEWVERRFSMAFAEMDD"
     gene            complement(36812..37663)
                     /locus_tag="AC06IO_000034"
     CDS             complement(36812..37663)
                     /locus_tag="AC06IO_000034"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIO9873258.1"
                     /translation="MCRFVAYLGRPTLLENVLVRPKNSIIMQSLHARETKYRTNGDGF
                     GLGWYSPEVSSDPALFTSVYPAWNDRNLLHLTAKIQSPCFFAHVRSASAGGVTNYNCH
                     PFIYGDWMLMHNGEINDFIDVKRHIRHLLDDDIYHWVKGQTDSEHLFGLFLQLAKGRD
                     INQLSVAADVLQETINKILEVIGQFGHSGPSYFNICLTNGKKIIATRYCSHEKKKPLT
                     LHYLIGYVLSTQGLWIKEEGNPAYVVVSSERLNDLDEGWEDVPPQHMILIDENKNIQL
                     RPLQTLG"
     gene            complement(37817..39853)
                     /locus_tag="AC06IO_000035"
     CDS             complement(37817..39853)
                     /locus_tag="AC06IO_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIO9873259.1"
                     /translation="MKFKIATCIVTMLSSFIVFSSNSNFSDNNTNDLLHLDWRDTSVS
                     PAVDFYTYANGNWKKNNPIPADYASWGSFYVVNEKVQNIIHQMLINASKNTHAKPGSI
                     EQKVGDFYFSGMDVDSINKSGITPLKPEFTKIEEIKNLKDLQAEIAHLHKIGVGPLFN
                     FGSMQDFKDSTSMIGAVMQGGLGLPDRDYYLKKEAKFKQVREAYVNHLTKMFELLGDS
                     PEMAAKEAKIVMDIETQLAKASMSQVEQRDPHAIYHMMTINELDKLTPNFSWSQYLSA
                     RGQGKIKKINFAMPDFFKAMNELLQSVSLNEWKIYLRWHLIDAFAPYLSKPFVDQNFK
                     MVSALTGTQKLLPRWKRVVNTENGALGFAIGELYVEKYFSPESKQKVLDILKNIRAVL
                     QEDIKTLSWMSPKTRDAALKKLDLMEERVGYPTKWWDYSSLKIDRGPYVLNVIRANEF
                     LINRDLDKIGKPVDRSEWAMTPQTINAYYDPSMNNLNIPAGILQSPFFDPKAPAAINY
                     GAIGYVIGHEMTHGFDDQGAQFDGYGNLKNWWAPNDLSKFKNATQCIADQFSKYVVDG
                     DLHVQGQLVVGEAAADLGGMILAYKAFQRSKEYKNAKTIEGITPDQQFFLGTAHVWAM
                     NVRPEQLRNQVTTDPHPPAVYRVNGSLANIPQFQEAFNIPNGSPMISKNRCIIW"
     gene            complement(39997..40911)
                     /gene="lpxC"
                     /locus_tag="AC06IO_000036"
     CDS             complement(39997..40911)
                     /gene="lpxC"
                     /locus_tag="AC06IO_000036"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIO9873260.1"
                     /translation="MIKQRTPKKVIQATGVGLHSGEKVLLTLRPAPVNTGIVFRRVDL
                     SPVVEIPASYEYVGDTMLCTTLHHGKVKIATVEHLLSALAGLGIDNAYIDVNAPEIPI
                     MDGSAAPFVFLIQSAGIREQNAAKRYIRILKPIRVEENGKYVQFLPHKGYKITFTIGF
                     EHPVFNDRPQTVSFDFSGTSYVKEVCRARTFGFLSDYEKLRECDLAKGGSLDNAIVVD
                     DYRVLNEDGLRFESEFVTHKVLDAIGDLYLLGSSLIGAFEGYKSGHELNNRLLRELMI
                     RQDAWEYTYFDTENYLPAVHPEYYPVEA"
     gene            complement(41147..42343)
                     /gene="ftsZ"
                     /locus_tag="AC06IO_000037"
     CDS             complement(41147..42343)
                     /gene="ftsZ"
                     /locus_tag="AC06IO_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIO9873261.1"
                     /translation="MFELMESSQQENNAVIKVVGVGGGGGNAVEHMVAENIDGVEFIC
                     ANTDAQALRGSSAKIHIQLGDALTKGLGAGANPQIGREAAEEDREHIKEILSGADMVF
                     ITAGMGGGTGTGAAPVFAEIAKELGILTVAVVTKPFSFEGKQRALAAEEGIRRLAEHV
                     DSLITIPNNKLLSVLGKNISLLNAFKAANNVLLGAVKGISDLITRPGLINVDFADVRT
                     VMSEMGMAMMGTGSAVGEQRARQAAEAAIASPLLEDVNFSGARGILVNITAGLDMSIG
                     EFEEVGDVVKEFISDDATVVVGTVIDPEMTDEMRVTVIVTGLGDNRQRQQQPQQPLRA
                     RLVETTRSDGSLDYQQLERPAVIRKQAQTGTGTTATKQTNNGNENVPDVDYLDIPAFL
                     RRQEEA"
     gene            complement(42538..43776)
                     /gene="ftsA"
                     /locus_tag="AC06IO_000038"
     CDS             complement(42538..43776)
                     /gene="ftsA"
                     /locus_tag="AC06IO_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIO9873262.1"
                     /translation="MAKKIEKNIITGLDIGTSKIIALIGEVTSDGTIEIIGIGRHPSR
                     GLKRGVVVDIETTVNSIQRAVQEAELMAGCEVRTVYAGIAGSHIRSLNSHGIVAIRDQ
                     EVSQADVERVIDAAKAVAIPADQKILHILPQEFIIDHQGSIREPIGMAGVRLESRVHI
                     VTGSVSAAQNIVKCVRRCGLDVIDIILEQLASSHAVLTEDEKELGVCLIDIGGGTTDI
                     AIFCEGAIQHTSVIPIAGDQVTNDIAMALRTPTKAAEAIKVNHACALPELANPNQMLE
                     VTSVNDRPGRKISAKALADVVSARYEELFSLVRNELRRSGFEDRLAAGIVLTGGASNV
                     RGAIELAELCFEMPVRKGCAHYVSGLTEATENPSFATGVGLLLHGFQQQYESGYNVPA
                     MNDNGKSIWSRMKEWFQGNF"
     gene            complement(43776..44495)
                     /locus_tag="AC06IO_000039"
     CDS             complement(43776..44495)
                     /locus_tag="AC06IO_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIO9873263.1"
                     /translation="MEVNSVGLRYLTIMGLLILSALLLAGRLGYLYLADAERFPITTI
                     KVAATYEHITHKELENVLAKYLDASFFLLSVKGLQSELNSMSWIDTAYVERVWPDTLK
                     IKLTEKKPVAIWGDALMTRDGKLFNQGSVPSDLDIPKLKGPQSQQLEVLQVYEKLSKI
                     LSSYGLNASGLYLRDNQSWVLLLNHSVKIYLGKKELEERLLRFCKAYPAVFAEKADQL
                     AGVDLRYPRGMAVQWKQQMGR"
     gene            complement(44511..45605)
                     /locus_tag="AC06IO_000040"
     CDS             complement(44511..45605)
                     /locus_tag="AC06IO_000040"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIO9873264.1"
                     /translation="MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAM
                     DKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLINGRLAVDAEIVFPVVHGPLYED
                     GCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
                     QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVG
                     REIELAVLENSIPCGKPRVSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSL
                     EEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPGFTSISMYPKLWQAT
                     GIAYPDLLDELINLAMIHHNCRQHLVTNYL"
     gene            complement(45589..46515)
                     /gene="murB"
                     /locus_tag="AC06IO_000041"
     CDS             complement(45589..46515)
                     /gene="murB"
                     /locus_tag="AC06IO_000041"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIO9873265.1"
                     /translation="MSITGIDSSHVTESQGTLLFNEPLAEYTTWRVGGPAARLYKPAN
                     IDDLALFLSRLPFDEPLLWLGLGSNSLIRDGGFSGTVILTQGCLKEMTLLSDNCIRVE
                     AGVSCASMARFSARNNLSGGEFWAGIPGTMGGALRMNAGCHGGETWQSVIEVQTINRR
                     GEIRTRKPEEFEVAYRHVAGLGDEWFISAKLQLSPGNKETSLQLIKDLLAHRAKTQPT
                     NEYNCGSVFRNPPGDFAARLIESCGLKGVSIGGAVVSEKHANFIINHQGTATAANIEA
                     LIHLVQTKVREQTSIELIREVHIIGDANVQTR"
     gene            complement(46524..47933)
                     /gene="murC"
                     /locus_tag="AC06IO_000042"
     CDS             complement(46524..47933)
                     /gene="murC"
                     /locus_tag="AC06IO_000042"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIO9873266.1"
                     /translation="MNNSEQFLSPRMGRVEQIHFVGIGGAGMCGIAEVLHNQGYRITG
                     SDLGESGTVQRLRSLGIQVYIGHRLENIKGADVVVRSSAVDFNNPEIVAARELMIPVI
                     PRAAMLAELMRFRHGIAIAGTHGKTTTTSLVSSLLAEGGLDPSFVIGGKLNSCGANAQ
                     LGKSAYFVVEADESDASFLFLKPMMAVVTNIDADHMDTYEGDFEKLRTTFLEFLHHLP
                     FYGLAVVCLEDEEICRILPAIQRPTLTYGFKEEAHYRAINWTQKGMLSEFVVVRPAPH
                     KQLTIQFQYPGRHNVLNALASIAIATELGVDDDSIVRGLQKFQGVGRRFQMLGEKQFE
                     KGAAIIVDDYGHHPQEILSTIDAFRRVWPERRLVHVFQPHRYTRTQSLHRQFVDVLSL
                     SDELLLMDIYAAGETAIPGVTSENLANEIRSRDKRVTIVSEQSLKATLDEFIKDGDVI
                     LMQGAGSIGQMAVNLMKNM"
     gene            complement(47943..49118)
                     /gene="ftsW"
                     /locus_tag="AC06IO_000043"
     CDS             complement(47943..49118)
                     /gene="ftsW"
                     /locus_tag="AC06IO_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIO9873267.1"
                     /translation="MRPRHLNQRGKPVSRPISLYDKWLIGAVFGLLIIGLMMVASSSV
                     MISTKYFHQPFHFLIRQACYLFVGLLLALIVVRTDSSFWEKISMPMMIGCVFLLLIVL
                     IPGIGKSVNGSRRWLALGPIGVQVSELTKLAMIFYLSGYLVRQQEAVCESIFGFIKPM
                     AILAVVSVLLLLEPDFGATVVISGTVMAMLFLAGVKLRYYFGLMLVVVTALALLAVSS
                     PYRVARLTAFLDPWADQYNSGYQLTQSLIAFGRGGWFGTGLGESIQKLLYLPEAHTDF
                     LFAVIAEELGLFGILVVITLYSILVIRGLNIGYTAYTQERHFASYTAYGLTIWLALQA
                     SINMGVNAGLLPTKGLTLPLLSYGGASMVINCIVIALLLRIDHENRWQSLGLRPLTA"
     gene            complement(49124..50467)
                     /gene="murD"
                     /locus_tag="AC06IO_000044"
     CDS             complement(49124..50467)
                     /gene="murD"
                     /locus_tag="AC06IO_000044"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIO9873268.1"
                     /translation="MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGVAEF
                     QNEFPDVPIYLQQTPDEVISQVTDVITSPGLALDTPVLERARQAGASIYGDIECLARE
                     ISAPVIAITGTNGKSTVTTLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLE
                     LSSFQLDLTYSLSPVVATILNVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVY
                     TVPHQSCQADIKCISFGKDAPSMGNWGLIEQENTIYLAKGMERLLPVESILIKGVHNW
                     MNALAACALAEAAGISMQHILNVLKTFPGLPHRCQWVREVDGVGWINDSKGTNIGATI
                     SAINGIGGSMQGKIVLIAGGQGKGADFQELAQPVSEFVRSIVLIGEDADKIESALAKV
                     VPVVRASSLEGAVTIAKTCAKPGDVVLLSPACASLDMFRDFNHRGDVFTSSVRGL"
     gene            complement(50481..51566)
                     /gene="mraY"
                     /locus_tag="AC06IO_000045"
     CDS             complement(50481..51566)
                     /gene="mraY"
                     /locus_tag="AC06IO_000045"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012220098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIO9873269.1"
                     /translation="MLYWLTQLLQGQYHAFRVFQYLTFRSILASLTALIVGLLCGPLM
                     IRWLRGLQIGQMVRSDGPQTHLSKAGTPTMGGVLILLAITVSCLLWCDLRQTSLWLVL
                     LVTLANGLVGWVDDYRKLVLKNSKGLPGRWKYFWQSVIALVAVSYLYWNASLPVHTQL
                     TVPFFKTVTWDLGVFFPVLAYFVIVGSSNAVNLTDGLDGLAIMPIVMVAGALGVFAYA
                     SSNAVYSNYLGIPYVPNTGELTIFCSSIVGAGLGFLWYNSYPAQVFMGDVGSLALGAA
                     LGIVAVVVRQELVLLIMGGLFVIETLSVILQVGYFKYSGGKRLFRMAPLHHHFELKGW
                     SEPKVIVRFWIITVVFVLCGLATLKLR"
     gene            complement(51681..53018)
                     /locus_tag="AC06IO_000046"
     CDS             complement(51681..53018)
                     /locus_tag="AC06IO_000046"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIO9873270.1"
                     /translation="MNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERF
                     DGHDFIKEAEAKGASAAVVNRLIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPIIAL
                     TGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPG
                     EIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAY
                     AHFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNGRVEIELQVPGLH
                     NVRNALAAAACCYAVGISGNDIQRGLAHFSGVKGRMTMLSGQNQSTVIDDTYNANLRS
                     VLTALEVLSGRPGRKIFVFGDMGELGEWTTQHHREVGIAARQLGIDKLMSCGLHSELA
                     SQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG"
     gene            complement(53259..54038)
                     /locus_tag="AC06IO_000047"
     CDS             complement(53259..54038)
                     /locus_tag="AC06IO_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HIO9873271.1"
                     /translation="MQANWSLILNVLLLIGVLVAIGRLMKTRRQSLNQEHYQPSLGQV
                     ENNSYGSQSYNDEIIAVRKVNREVPLDFENFDGSEKRQAVRKPSVKTEQPQLIPEEEE
                     CESNERTEPNSDCSSSTAMVFLLAKENRQFAGYELLQTVLAAGLRFGERHLFHRHQFT
                     NGQGPVLCSLAAATATGVFDLQNIGAFSVRGLCLFMQISNNPEINAERFSVMLDTAKQ
                     LSEGLDAYLLDDQRKPLTEERIERYHRYLKIEHLMDDCVTN"
     gene            complement(54043..57537)
                     /gene="smc"
                     /locus_tag="AC06IO_000048"
     CDS             complement(54043..57537)
                     /gene="smc"
                     /locus_tag="AC06IO_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIO9873272.1"
                     /translation="MHLKQLKLAGFKSFVDPTTVHFPSRLVAVVGPNGCGKSNIIDAV
                     RWVMGESSAKNLRGESMTDIIFNGSSNRKPVGQASVELVFDNSLGRLTGPFASYGEIA
                     VKRVVTRDGESFYYLNGARCRRKDITDIFLGTGAGARSYSIIGQGTVSRLIEAKPEEL
                     RVYLEEAAGVSKYKERRRETLQRIEQTRDNLTRVADIREELGKHLQRLERQAKAAERY
                     TILKEEEKICRAEIQALKWQEYSEKQTVKQRELQELAIRNEQQQSLLTSCYKERAVLN
                     EQVQDAIDQSQSIQDSLYHSGTEIARLEEAIQQQEREKKRLENDRQQMQVDSQLIEEQ
                     LKLDNEELHNCKLKAQQLEEHLRHLKMEFKEQESLWQEKQKEQSQWEKIWQEVQINSN
                     TIKNQYQLTQAKLQHLEQKRHQVLVRLEKLNLEQSAISLDDLQKNKIKLQEKHFQLKE
                     TQEFDEEQLRQANETQSDLQSQLQNTEQQLHALQDEFHRLNSELAALKAAQIAAKKEN
                     RPNKDGLAEWSDKPRLMDVLKVEPQWQSSCEIVLGDDLIAYVLDDFAELWSRWQFCED
                     HKENVVTMRALNGEKQERPRLADKIQGLVPSTVNHLEHIYAADNLEEAKAWFPDLLAH
                     ESIVTVNGIWLGKGWIKLPRQKEQDEVGILARQQRIIELNLSVESLQQKIESIRLARD
                     SYQARLQENLNFIKLHQLNLNASNEALRNHIIAVNSQEQAIFHAEKQKDAITNEIIEL
                     ESSLEELNAEQLEMADKIRELEGQYQASELQFKRFINEKDTWNSVLVEQNKQVESVRA
                     LLHQSELEYDREVTKIQQITDRISREKERLQRAQMRLEELTQHYLQSVAPEAEFKNQL
                     AALLMKHGEIELMLTLSREKVSQLKLQLEDQEKLILNYDQEVKRIQELMNQIQMEKQA
                     LSVRASSIQETLDEANLQAQSLLERIPSGVTQSIKEDELIAILEKIKRLGAINLAAIE
                     EFAAEQQRKVFLDEQYNDLSQALSTLETAIEKMDNETKSRLENTFDEVNTSFKALFPR
                     LFGGGRAQLELTCDNLLEAGIVVMAQPPGKRNSSIHLLSGGEKAMTAVALVFAIFQLN
                     PSPFCMLDEVDAPLDDLNVSRFCDLVKEMSQFIQFLFITHNKVTMELAEHLVGVTMRE
                     PGVSRLVAVDVKQALTME"
     gene            57712..58392
                     /locus_tag="AC06IO_000049"
     CDS             57712..58392
                     /locus_tag="AC06IO_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HIO9873273.1"
                     /translation="MKQQFYKLPQILISLCLLLILSTPLQAEPPNLSLIRKEIKNYYD
                     SGLYYHELERTIKLAQEYIHQQYLINKNNKHPQKLAIVLDIDETSLSNYDKMVKRDFT
                     GSKEQIHKEILAANSPAIKPMLTLYKNALKQGIKVFFVTGRQESERDATRTNLIKAGY
                     TKWAGLYLRPNGYSSSSIIPFKSKAREMIAKKGYTIIASIGDQYSDIQGGYTKKGFKL
                     PNPFYYLP"
     gene            complement(58494..59555)
                     /locus_tag="AC06IO_000050"
     CDS             complement(58494..59555)
                     /locus_tag="AC06IO_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIO9873274.1"
                     /translation="MSKLINLAVLSFSSSLAFAGPLTLKGEVDVHITPKNPSGVAQSL
                     TFKLPKYELSTEAKSYLREQLSEYPKNSINTASFSSELPRKVKLGMQLTPVLDQGYHG
                     SCVTFAVTAAIDAALGAGDYISQLCNLELGSYLAIHDKAKASGWNGSFGYWVLQQISE
                     YGIISQNYQKLNGCAGVREYPLEDENNEGKPMSDSEFLAHSVPVSNLISWEALLKDEE
                     SFSAKADMNQIVYQIKEELAKGNRLTIGMLLDVFVGDAGAVGTNRAYHDTWMLTPEIV
                     LDAMNGMIYAGHELVITGYDDDLEVMDEEGHVNKGVFTLRNSWSKFAGDQGDYYVTYD
                     YVKFLAMEVMAIRMKEKAA"
     gene            59865..60677
                     /locus_tag="AC06IO_000051"
     CDS             59865..60677
                     /locus_tag="AC06IO_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HIO9873275.1"
                     /translation="MRTLLSCFLITLLSFGLIINEASAKRFGGGRSFGVQRSQSSLFS
                     SNKTHNTSSLTQKANKSRWGGVLGGLLVGGLLASLFMGNGLANGLITWLILGSVIFFV
                     VSFLRRKMNPGFQATQSGAFRQNSFEQFTQNYGNRNSGGAHFTQQETSFDADNFLRDA
                     KVTFIRLQAAYDQKNLQDLQTFTVPEVFAEIKMQLDERGDAPNQTEVISLDAKLLDVS
                     KQSLSMIASVQFTGTIKENGEISNLDEIWHFRQFDNRKEWVVGGIQQEVFQP"
     gene            complement(60751..61233)
                     /gene="greA"
                     /locus_tag="AC06IO_000052"
     CDS             complement(60751..61233)
                     /gene="greA"
                     /locus_tag="AC06IO_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIO9873276.1"
                     /translation="MSKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKE
                     NAEYHAAREQQSFNEGRIQELEAKLSHAQIIDISKLPNNGKVIFGSTVTICHVATGSE
                     LTYKIVGEDEADIKLNKISYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE
                     "
     gene            complement(61242..64445)
                     /gene="carB"
                     /locus_tag="AC06IO_000053"
     CDS             complement(61242..64445)
                     /gene="carB"
                     /locus_tag="AC06IO_000053"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIO9873277.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGTQAVRALKEEGYRV
                     ILVNSNPATIMTDPELADATYIEPVQWKEVARIIEIERPDALLPTMGGQTALNCALDL
                     VREGVLAKYSVEMIGATREAIDRAEDREKFRQLMIKIGLDMPRSAIAHSLEEAIQVQA
                     RLGFPAIIRPSFTMGGSGGGIAYNREEFEEICIRGLELSPTHELLIDESVLGWKEYEM
                     EVVRDKNDNCIIVCTIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDAAIKVLRAVG
                     VDTGGSNVQFAINPEDGRMLVVEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGKTPASFEPSIDYVVTKVPRFNFDKFPQTPDTLTTQMKSVGEVMAIGSNFQ
                     ESLQKAIRGLEIGRCGLHSLFHKEDMARLRGHLREPTPDRLWYIADAFRQNLSLEEIH
                     LESRVDPWFLAQIQELVLLERSIIGKTIRDLDKTTLHLMKHRGFADAYLAKLLGCTEA
                     EVRARRVELEILPVYKRIDSCAGEFPSDTAYMYSCYEGSCEARPESNKKKIMILGGGP
                     NRIGQGIEFDYCCVHAALSLRSAGYQTIMVNCNPETVSTDFDTSDRLYFEPVTLEDVL
                     SIVAIEQPEGVIVHYGGQTPLKLARDLEANGVKIIGTSSDAIDMAEDRERFQQMVKEL
                     KLHQPDNGTVRSEAEAIELANKIGYPLVVRPSYVLGGRAMEIVYQEEDLRYYLSHAVE
                     VSNDSPVLLDKFLNDAIEVDIDAVCDGKDVLIGAIMEHIEQAGIHSGDSACTLPPFSL
                     GVVVQQDLIEQIRKMALKLGVVGLINAQFAIQADDIYVLEVNPRASRTVPFVSKATGL
                     PLAKIAALCKVGISLKDQGLNQNHHTPSFYSIKLPVFPFIKFSGVDSILGPEMKSTGE
                     VMGIAKRFGQAYAKAQLGAGCHIAKRRRAFVSVRDADKTRVGEIAKRLIELGFEIIAT
                     RGTALALQAAGVDCRRVFKVAEGRPHVLDFIKNNEIDFIVNTTEGKQAIADSFAIRRN
                     ALQHKVSYTTTLSGAEAACLAMKYEDRETVTRLQDLH"
     gene            complement(64572..64844)
                     /locus_tag="AC06IO_000054"
     CDS             complement(64572..64844)
                     /locus_tag="AC06IO_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873278.1"
                     /translation="MNEMDILSLFYDEMTARGVTREQVFLSIEEDAAAMLTQKLGKPV
                     SVEEAQKLTDICIANEWLERTTADPYYKYLSLTEAGLQMLLSTLYK"
     gene            64957..66138
                     /locus_tag="AC06IO_000055"
     CDS             64957..66138
                     /locus_tag="AC06IO_000055"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIO9873279.1"
                     /translation="MQEIDVLIIGAGAAGLMCAIEASKRKRKVLVLDHANKAGKKILM
                     SGGGRCNFTNYYIEPNKYFSHNPHFFKSALSRYTQWDFIELVNKHKIPFHEKTLGQLF
                     CDNKSKDIVDMLLKECEQYGVAIYLNTVIEKIQKTNDYSFKIGTTKGKFHCHSLVIAT
                     GGLSIPTMGASPFAYKVAEQFAIKVWPTRAGLVPFTLDVLEKDRLSILSGIGIDSLVN
                     NERNQFREHILFTHRGLSGPAILQLSSYWNPGESICINLLPEHNLLESLKIARAEAPH
                     KQLNSVLSMYLPKRVVEVFIPQKLGEKKLADSSNKDLETISHLLQNWVVKPNGTEGYR
                     TAEVTIGGVDCHAISSKTMEANNVPGLYFIGEALDVTGWLGGYNFQWAWSSGWAAGQV
                     V"
     gene            complement(66173..66925)
                     /locus_tag="AC06IO_000056"
     CDS             complement(66173..66925)
                     /locus_tag="AC06IO_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2628 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873280.1"
                     /translation="MAFIRHLLEYLLHPFFLCLLTLGICIFLLYRRLAPTFTKVMLFV
                     VLLVLLAMSTGWLPSYLTYQLESRYPIVKRVNPNVKWVVVLGGGQNIGEGMPANDLLT
                     SASLKRLVEGVRLFRDLPDAKLLLSGGGSAGEQSEALLLAELTKWFAIDPEKVVIETV
                     SINTADQARELAAIVHDHPFYLVTSAIHMPRSMALCQQQGLHPIAAPTDFTFFWSTDT
                     WVKMVIPNSYNLAYTSIALHEILGKIWALGFR"
     gene            complement(66925..67995)
                     /gene="lptG"
                     /locus_tag="AC06IO_000057"
     CDS             complement(66925..67995)
                     /gene="lptG"
                     /locus_tag="AC06IO_000057"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIO9873281.1"
                     /translation="MSRKILDRYIAKTVLSAIGLVTLMLVGLQIFILFVNQIDDLGRA
                     DYGIVQAGVFVLLQMPYQVYLFFPIASLLGCLVGLGVLANHSELVVMRAAGISIGQIT
                     GAVLKASLIVILLITSLGETVVPVMAHYANEYKVSALTGGQALLTPRGVWVRNGNDFV
                     SIASVTRENTLRYVNQFRFDNQHNLKLARQISEIKYVNNSWIAYDVKQTEFAEDHTKA
                     YHFDTLPWDVPVKPEILKITGTNPDEMTLHELNRYLREQQRNHQNVANYKFAFFQRIT
                     QPLNTMVMMILAIPFIFGPLRSSTMGSKLLVGAMVGFGFHILNRFFGPVSTVFQWPPE
                     LAAFSPTLIFSLIGIYLMRRVR"
     gene            complement(67992..69071)
                     /gene="lptF"
                     /locus_tag="AC06IO_000058"
     CDS             complement(67992..69071)
                     /gene="lptF"
                     /locus_tag="AC06IO_000058"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIO9873282.1"
                     /translation="MIIFRYLTKEVFLTLIALTAILVLIFMSNQFIQYLNRAASGSIP
                     GFVIMKLLMLELPNLMGLLLPLGFYIALLLAYGRLYAESEMTVLRACGYGPRQLLKHS
                     FAMATVVLVVVAIVMIWASPVIAVERTKLIRSTGIKTLIQTIMPGRFHAINSGQQIFY
                     VQSMNRDHSQAEQIFMAKHSVQDNKLLWDVLWADKAFAEVNPQTGEEYIVLQNGKEYQ
                     GVPGHADYQIAEFASYKSKLPNPTVVMHDDARTAKTRDLWPLNNHDLKKAAELQWRIS
                     IPLMVLTLTLVAVPLSRVSPRSGKYSKLLPAIIIYIFYANFMFVSRDAIATGKIPWWI
                     GMWWVHVAVAILGILFIWRNQVKLA"
     gene            69275..70726
                     /locus_tag="AC06IO_000059"
     CDS             69275..70726
                     /locus_tag="AC06IO_000059"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIO9873283.1"
                     /translation="MNYGLTHTPSLNMSECLVLGVFSDLALPDFATAIDNEQQGLIKK
                     LCQRVPEPGNTVWQTDVEGHSLLIIQCGKKEEFSANSLQKRVGEITEALIKQRFSSAT
                     VCLPRLNQESSEWQLEQMIVQIDNLRYQLLDFKTKHAKSHKLESVIFHLPGATEKSLE
                     MAKAIVTGVEFCRDLANMPANICTPTYLGEQAISLSKQFDQISCQVMGPEEIKEMGMG
                     ALLAVAQGSDQPPRLIDIHYHGNKNSAPVILVGKGITFDSGGLSIKPANAMDEMKYDM
                     SGAASVLGVIKACALLKLPINLIGIIASAENLISGSAVKSGDIVTTMSGQTVEIINTD
                     AEGRLVLADALTYAERYNPDFVIDIATLTGAIIVALGNIATGYMTRDEQLAKSIERAA
                     NESQDKVWRMPLDEAYQDALESPLADMINAGFDRSAGSITAACFLSRFTEKYRWAHLD
                     IAGTAWISGKKRNATGRPVPLLIQLLRHVANSR"
     gene            70707..71141
                     /locus_tag="AC06IO_000060"
     CDS             70707..71141
                     /locus_tag="AC06IO_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIO9873284.1"
                     /translation="MSPIRVDFYLLASSLPESRWLVACRLIEKAYTKGHTIFVFCDNQ
                     QQAEYLDELLWTFKDDSFIPHNLQGEGPEPPPPVQIGYQKEPRGFTDILLNLSTNIPI
                     FYTRFKRVIELVSNVEHEKEVSRLHYKEYRNKGCALHTHSIE"
     gene            complement(71288..71605)
                     /locus_tag="AC06IO_000061"
     CDS             complement(71288..71605)
                     /locus_tag="AC06IO_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HIO9873285.1"
                     /translation="MFVTFSCDAYENITYFENVAKQLLLLMGHGGTIPGAIKSENVPD
                     ALHHLQQGLEKIKSENKLLEDDEDSEPDISLAKRAIPLIHLLQAAEKKDCDVLWSHSK
                     SPG"
     gene            complement(71628..72992)
                     /locus_tag="AC06IO_000062"
     CDS             complement(71628..72992)
                     /locus_tag="AC06IO_000062"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIO9873286.1"
                     /translation="MQAEVFYKSCGDRIVPVHLISQSQWDEGIENLTPMERNCFSVRQ
                     FKGKLGDYCFILNADGVIEKAFIGSGTGNQESALANAALLLPPGNYQFQDKLSRESGV
                     VWALAQYRFDEYKKYELQPRVLIVEANDRDYILSLANSLFLVRDLINTPTCDMGPESL
                     AHVVEELAKTHKAYFKQWIGEELIKDNFPAIHAVGRASALTPRLLSLHWGNEKHPRVT
                     LIGKGVCFDSGGLDIKPSSGMRLMKKDMGGAAHVIGLAQWIMVQRLPIRLQVLIPAVE
                     NAIGPNAFRPGDVLTMRNGLTVEIENTDAEGRLILADALAMACEEKPDLIIDFATLTG
                     AARVAVGTEIAAMFSNHDQLAQDIVAASNKVSDPVWRLPLFSPYEELLNSNIADMSNS
                     SSSSYAGAITAGLFLQRFVTKSIPWVHFDIMAWNAVSKPGKPEGGEAMGLRAVAEYLL
                     QTYG"
     gene            complement(72997..74568)
                     /gene="murJ"
                     /locus_tag="AC06IO_000063"
     CDS             complement(72997..74568)
                     /gene="murJ"
                     /locus_tag="AC06IO_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIO9873287.1"
                     /translation="MLTTDIMVPKKQSLIRSTSLVSLMTFISRIVGFARDMVLANFFG
                     AQAGMDAFFVAFRIPNFMRRLFAEGAFSQAFVPVLAEYQKTRSAEDVRTFIARISGYL
                     SSILTLVTVVGIVASPVIIFLFAPGFHHDSVRAELATQMLRITFPYLMLISLTAMAGA
                     ILNTYGYFGVPAFTPVLLNISMILAAVYLCPNLPQPVVGLAWGVLIAGIVQLLFQLPF
                     LYQRHLLIRPRVVRDDPGVNKVLKLMIPALFGVSIAQLNLMVDSIFASFLKVGSVSWL
                     YYTDRLTDFPLGVFGVAIATVILPHLSRKHTEQNINQYSRALDWGLRSILLIGIPAGL
                     GLSFFALPLIASCFAYGKFTAYDLLQTQKSLITLAMGVPAFMVVKVLASGFYARQDIS
                     TPVKVGAIAMVVNTLLCFVFVWHLAHAGLTLASALAGYVNCAILLFLLIKRGLFKPSP
                     GWLKYSMQLLIANFAIGVYLYFMSGTATYWLGFSPLMRLSLLLAHVFVTVVIYLLVLG
                     IIGLRPSHFRGQIKD"
     gene            74925..75191
                     /gene="rpsT"
                     /locus_tag="AC06IO_000064"
     CDS             74925..75191
                     /gene="rpsT"
                     /locus_tag="AC06IO_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIO9873288.1"
                     /translation="MANIKSAIKRARQNVKLRQHNASARSMYRTYIKNVLKAVESGDQ
                     EAARAAYTKAQPVIDKAANKGLIHKNKAARIKGRLVARLKAMAA"
     gene            complement(75252..76463)
                     /locus_tag="AC06IO_000065"
     CDS             complement(75252..76463)
                     /locus_tag="AC06IO_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2637 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873289.1"
                     /translation="MFLTINDLQSQIQLAKKRLYVLAKNWLDTQKEAINSKDKANWLN
                     AFQASTKEAMQEQLKATGFPLIRNAILVNQSEDELQQHSLEQSSSLIHSENLPEQFNS
                     RTVPRVIYVCHSGENNQVIKRLCQLTTLMEQLHTLEELTFEVQTNVHFRGLNLAYRMA
                     RSINKEWRDVEKGRETVEYYAERLQIAEKQYKLASDFCHECLGIYKEQLDARIMYFNE
                     SLNFFVECLAATFSSVKDDRYWLTKFLLPSLPWHSMLNEVATWLFTTNGFTNPTIDSI
                     EVNRSHLKAITEQFAKLMMAPFCSLEKELQGKIEKVENEQKIARENVYAMSSELDIVI
                     HMQSQRKLAALLFPAQPLSMLGFFQGVAAAMGRDYFYSTFIKGNQSQEPRITDDEKSL
                     IEASNYSNGLH"
     gene            complement(76815..78197)
                     /gene="mavV"
                     /locus_tag="AC06IO_000066"
     CDS             complement(76815..78197)
                     /gene="mavV"
                     /locus_tag="AC06IO_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavV"
                     /protein_id="HIO9873290.1"
                     /translation="MKYAVTFCTTDQEVGSNPLWHSCLLLSKMDETSKLLEVVDNWGF
                     YGLPTTNRNSSWLDQLKIKMGLDVDLIGNHGMLRHEELRFLDQGCGLRGVTFELTKDD
                     FELLQKKCLQMATDQETAINEFVETQGIKGKPPEKTRIYPHEQFSRLIYATEKIKAEQ
                     QGREARLKPFELRLSWGLFGPSLALSQNCKTQALSLLSYVLSKEQIERLTEYGEHPTV
                     PRRSGPMESIFLHSTGPLREHRKSSGDVVHYRDLKDPGVKLYWTVPPQELEALSEETI
                     KLFQIDGEYCEEVKAIVRKLQRLEWLLINSNLPEKYRPYKDELINKVVGCYKAFSFIE
                     PKQEEPKLGGWYGWALSLFSAPRSAEERTLQGKIKKAKSLFNSIYMAIVDNYQIDDNA
                     PMENKNILPYEETGENRFENMVEAVASYLSRKDKINLCKIIGRTYCEDHVELEDNEGK
                     IALSPQPLAT"
     gene            complement(78605..82207)
                     /gene="enhC"
                     /locus_tag="AC06IO_000067"
     CDS             complement(78605..82207)
                     /gene="enhC"
                     /locus_tag="AC06IO_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry protein EnhC"
                     /protein_id="HIO9873291.1"
                     /translation="MKSLVPWVCVLVASAGQQAFANDAFNAYRLGNYNEAAEPLIIKS
                     GQDAVAEYYLGRLYLYGYGQLKNNRVAMRYFTKSAEKGYLPAVLLMAKYSLLHEKNPE
                     EAARWFKQAAAVGDVSAQMYMAAAYLYGVGVKKNTDIARRYYIDAAKNGNPIAQYTLA
                     EYFLESKHAGNKKLGIIWLNKAAENGDPKALTELGRFYVAGQMVAKDTAKGVELLNKA
                     ASEQYTPAMLELGKLALMQNQYDEAIQWLNKAEEQSTEATLELAHIYLQDKSPVYDPK
                     TGFLLVLKAAQNGSVQAQKELANLYQKGIGVAADEELSKQWTLKAAENEKKNAEPPVL
                     AQAALWLSNDRTDKLEETAYQMNGIFSPWNNPSALRNNSYNQAPKLETISRQDIFKPQ
                     FELTQPNSIPISNYYDALISKQVEFQSNQWAYPTYRLNPFVEALERANSPVVQKLNLP
                     VPYADANYDDSEVGSFDLMDIWTGDWKRTVNYMSVVNYLYFRAILGNAQTQFEIGQLF
                     QYGIGLMQDDASAIIFYENAAEQKHLGAEYNLGILYLKRGKDENDYQQALNWLTDSAF
                     KGNKRAQYVLARILRQGIVGPDGKEYIKANDEQAMAMLYLSAANDYGPAEYELAEYLA
                     RDYNNGLSVDVRKQKIALIRKLYQGAVKNKVAEALLPLAFYNAMDDNRQKQEQAFKVA
                     EEQAETGNENAALLLGMLYDRGIGITADPAKAMYWYQQAGQNPVSQFILGTYITEGKG
                     VAQDKEKGLDLLKQSADSQFSYADFNLAVLKQQSGEDFLPNLIESYKLGNSHAGIVLA
                     DYYLSQSSVPEQMNQAKEIYKGLADKGDQYAQLKLAYMLQKGLGAAPDLAGAQHWYTA
                     SAEQGNPEAQYLLGQFYQLGETGEPDYNLAKHWYQKAAKHLSKADVALGFIYETVDDN
                     YAQALKAYENAAAKGDMLGAYNLALMYEYGKGVPVNYSKALSLFKEASEKGAPEAMSQ
                     LAGMYFYGLGQPRNEQQALVWYKKAASLGNGNALYALGLLSETGVGVKLDFPDALRYY
                     QDASDKGNEKAMLALARMYHYGLGVEKDHKRSASIYQKLAQKQNAYAQYQLGTYYIEG
                     TAGERLPEKGRELLQQASENGSLQARRVLQRLDAQTQGKVSYVEPVLFNNVPKFDGQS
                     ADMIYFDALNEWNHGEEVLSRMILQRLVTQYPNFIPAKRAYEQLNQAKLMNNLG"
     gene            complement(82234..82800)
                     /locus_tag="AC06IO_000068"
     CDS             complement(82234..82800)
                     /locus_tag="AC06IO_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase IV"
                     /protein_id="HIO9873292.1"
                     /translation="MNLKSRSSITAIIVILLTGALNMSAYAAKSNKTATAEDKAEKKD
                     NGKFPLGCTAVGYQQNLKVLTLLPGKEGALQSMYFIYNTLPETVSLYQMHDKESLYSY
                     WLNHSIRGNSWAVLATGEKEVKFICTLGDGKKSYGKILDCADVIKVCEYVNVKFGLNN
                     KGNFWIVDSNTRNGAVSQVVHYGIIPGV"
     gene            complement(82797..83519)
                     /locus_tag="AC06IO_000069"
     CDS             complement(82797..83519)
                     /locus_tag="AC06IO_000069"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIO9873293.1"
                     /translation="MKKILLTTFMMSMVTVLNAGHFYGTALCAYPQYECIKVTGGQSW
                     EKLFPDPVQRDIVQRVNRTYNYLWSGREIAVPKNLANINIFDVSPFPLKIQSENEKQI
                     IVDQEKLAWGAYDAQGNLVWWGPISSGSDKCSDSNKVCRTLTGIFRVFSKENVRCTSD
                     VFPIGRGGAKMPYCMYFHKGFALHGSDDIPGVRASHGCVRMFIQDAKWLNENFVELSS
                     ERNNFMGTKVIVRPLNDSENKK"
     gene            83692..>84943
                     /locus_tag="AC06IO_000070"
     CDS             83692..>84943
                     /locus_tag="AC06IO_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIO9873294.1"
                     /translation="MSPLKSKIKTKSFNKLTKTQDITNTIERLFANSQDSIIVFNQQG
                     KIIAINEKAAALFKKSPDKLIDKPIWKLFKLNSFAIKRQFIQAKRCFLLANEGIAQQF
                     TWLESKAQKPVLAYHILFNKTEIEDTSIIFTKLTDILKSKTIEWILWSLTKISNHQEI
                     NEIIDQILQLTSDVFAADYTSVSFINNQQIARTISYYKFGKKEENISFSLINTPCAKV
                     IQKKAICYFNDVQKQFPNDKLLKRMNINTYLAGPITNPQDEVIGIMTILCKRKVEFDT
                     LNNTLFGLFLSRINSEIERLINLRKLEFLASIPQQNPNPIIRILLNGDVVFTNDEGKI
                     ILNYWIKLFKGLPVELLKEAQRIQKSNQSTRIEMEIEDKIYLFTLVWIDEFKQINIYG
                     TDITQLKNTQNAMLNLTRRDTLTQI"
BASE COUNT        26500 a        18580 c        15222 g        24641 t
ORIGIN      
        1 tgatatatta atccaatcag gctcaacaga acattttttt aatgcgttaa caattaattt
       61 ccgctcttca actgttactg acttatttaa taaaacaggt aaaagttcat tttttatact
      121 atcaacaaaa tcattagact caataagttc aatagattca agaagtaaat aggataattg
      181 aaaagtggtg cttgaggatt ttataatacg tagaatatca tcgcgtaatt ctggtttcaa
      241 caagccattt cctagactca cgtaccaatc atcagatttg aaataaggat cttcttcagc
      301 aactttttct aaatatgtta aaagattttt tgattgttgc agggttagag ttttaggatc
      361 accgtatctt agaaaaccat atggatctct tttgataaac atatctctgc ttggttggtg
      421 tagagtagca agccaagcat gcaaccccct gagtgcggtg ggtacaatag tgtttccaca
      481 ataaagtact ccttctagtc gttttattga tatcttatct attaatgcat tagacaacca
      541 tttcgcagcc ataaattctg cgacggttct atgacagggt tcaataatat tatttccaac
      601 tgagcgaaaa agcattgttg ataaaacttc tcgtgctgtt gtttcacagt aagattcatt
      661 agtgaactcc gctaatcctg gcgctaatac attttcacta ctttcaatgc aaatacctga
      721 ttttcctgag agtaaaagtt gagcgcaaat aaaaccagct gcatcttcta atttagtaca
      781 atttgacgtt actttaatgg aggtatgcgt ttgattgtgc tccttaagaa gggtcgaaca
      841 tgatttttca tatagttcta gcctactatt aggccaacca tcattttgct gaatagcttt
      901 taaaaataaa gttaaatttt gagggtttct taataattcg acaaaatctc ttttctttgc
      961 ttccaacaaa aattctctac cattttgtcc accagtttta atgtttgcaa actctataat
     1021 ttcttgatca ttcaacggta ataaatgtcc gatgataggc ttcgtacacc atctgtcttg
     1081 aattgttttt atgttgactg taccttgcca atcagcggct ctgcaggtca gaataaaatt
     1141 tggaaaatca tgatttgcta gtttacttag tatccttctt gaaagagaaa ctccaggctc
     1201 ataagcggta atttcatcaa taccatcgat aataattttt gctggtggat taacgctctt
     1261 aatttctgga aaaagatcaa tagatgaggc gttgtatacc gttactccag aagatttcgc
     1321 aaaattttta actaattcac ttttccctgc accaggctca cctaatatta taagaggagc
     1381 attaatagca aaaaactcat tgtcagaaca ttgttcttca tgaccttttt cattgatata
     1441 aactatgcgt cgaggtaaat aattcataaa ctcatcttaa attggctgca tttatattat
     1501 acgtgataat cagactctac cccaaataag gaaggtgttg agatttcaga accaaagtac
     1561 acccagaagt acacctagca gaatttttga tttgaaatga gttttaactg aatgatgcaa
     1621 attactgatt ctaaagagga ttttatggtc gggacggaag gatttgaacc ttcgaccact
     1681 agcaccccat gctagtgcgc taccaggctg cgctacgccc cgattggtta ggcatgatat
     1741 ctaaataagg ctaaaataac aagggttatg gtttactatc atgagtgaaa tataagttcc
     1801 tggcttgcct gagttttatt ttcctgcaat aggccctact cgatttctgg tatttttggc
     1861 catgacttta gaactttctg aagtaccagc gcatgattat gttgaagatc ttttgccgca
     1921 taaaacaaag tgactggtga agtgctcgat aacgccaaaa cgtgttcaat caattcgggt
     1981 ttttctttta attcccgagc gtaccgcttt gcaaactcag gccaatgctc acggtcttga
     2041 tgaaaccatt ttctcagaga cgggcttggg gctatgtctt tcagccacat atccaaagga
     2101 atactgtctt tttttattcc ccgaggccat aatcggtcta caagtatcca tagcccttct
     2161 ttgtgttccg gtggagagta cactctggat aaggtaattt cgggtgccgt catattatgt
     2221 catccgtatc attggaaaaa tttcagggcc attttgatct ctttgcttaa cgtgagttat
     2281 ggataagata gggcgttatc tccaatcgtt cgggctgagg aagcattaat gctgtctcga
     2341 agccttgtat ggaatttaat gtttcgtgcc aaggcttcga gacgatgctt atgcatccct
     2401 cagcctgaac ggctcctgga tatcgactgc ttcttatata atccttatcc ataactctcg
     2461 ttaattaaga atagcatgga aggaggagtt ttaccgctca tccttacctg agaagaatta
     2521 tacccaatca ggtaatttag ggcctcggct aatatcgaat ggatttggca gggatgattt
     2581 atattcttcc cttgacgaat ctttttccat taccgctccc aatttttccg gatgttttac
     2641 tgattggttt tgatgttgca aaactggtgc gttgccgggt aacaaatttt tatccatcaa
     2701 ttgttgcaca aacaaatcat aatatttagg gctgggtatc tttatcgtca aactattacc
     2761 ctctctggcg agagtcattg tttccggagg aacctgtgga tcttttattg aatttttaaa
     2821 aagattaaac tcctgtttaa tcgtatcaaa caactcatcc agagccttct gttcttcatt
     2881 gcttagctcg cgcttgcctt gcaacaccag gaaaccaagc tccatgttca gagtcatatt
     2941 gactaaagca agagggtctt taaattcaaa aacaaaatca gcatcctctg gcaaatgcca
     3001 taggccacta tgcaatagta gttgatctat gcttggaaca ttggcaggat cctttggtgc
     3061 gagcaactga tgattattac cctctaattt gtcgtcggaa gcgctgtctg atccaccacc
     3121 ccctgaaccg ccgccatggc cacgacaaaa aggtaaccct aatgaacgac agatactgca
     3181 gggattattt tcaattgcac gtgcacgttc atctttttgc gtttcttctc ttttctttgc
     3241 cacaaccatt attttaaacc ttggagttac taacaggcag atgataattt aaaatgaatg
     3301 tttcatttgg caacttggcg aattttgcaa aaaatttaca ataaactaca ccttgaacct
     3361 cgttacggag atcaaggcac atttttggga ttagggttca aggtaggagt atagcgagct
     3421 tctctttgct cttcaatttc atcatctgta ccgtcttgtc ctgttgtttc atatccaggt
     3481 acaccatcac cgaccagatc cacttcatca ccacgggtga attgattatg atccatatca
     3541 ttatctgacc cttccaactt catttcctca gtcaaaatat ctttaggatc caggactgtg
     3601 tctggttctg gctctaactc aatcatatgg cggcccttaa atataatctt ctctatattt
     3661 aagtgtagca gggatataaa gataagagcg ggactttggc aaggagcagt ttacaaggta
     3721 caaaaatcaa tccggtgata tagaatttta aggcagacta aattggggcg taaagtctca
     3781 ttgcccctaa agcaaagaga ctgcttttga aaaaatataa ttattttcta caataggctt
     3841 tttattcttt gtgcttccag tttggaatga gatatctgta tatagcgatt acaactattc
     3901 agctcagcat attgcaaaga ataaatggca ttatccagtt cttgttttgc tgcagaatga
     3961 acatcctcta caatccagtc cgccgctgaa tcaatgtgta ttgaagcaat caccaatttt
     4021 tccgcacagg tttttttagc ctggctggaa accaaagagg ctactgttga agccaagtca
     4081 tgacactgtt gtaaaatggc ttctttattt tctgatgaat ttgcataact gcttaaagtt
     4141 agaacggaca aacaggctat caaagttgct tttaacttca tgttaatcat attaaactcc
     4201 tgtaattgtt tgtataaaac cgttttacat cgaatatcca taaaataacc catgccaggg
     4261 ccctaccttc ctaattcagc accataatct ggcgggcaat cgctcaactc taaaatcaaa
     4321 aaggtttaag cagttcctct ttccataaga aaaaggaact tgaaccgtaa tcattactga
     4381 ttaagaatag ttatcttgtg gtattcagaa ctaactcgat aaaagcagaa aaatccttaa
     4441 ccaaaagcat taattcccgt tatatgtctt cccaagacca aagtatgcac attatcggta
     4501 ccttcataag taaagaccga ctccagattc agcatatgcc gaatgacatg atactcaaga
     4561 ctaattccat tcccgcccat tagattccta cattttctcg caattttcaa tgcttcacgg
     4621 cacgcattac ccttagccag agaaatcata gtcggtgtct cacgatgctg atcctttaaa
     4681 cgcccaatct gcaaattaag gcattgtgct ttaataattt ctgtatacat atcagccaaa
     4741 tctttttgaa tcaactgaaa cgaggccaaa ggtttggcaa actgcttgcg ttccagcaaa
     4801 taatcccgag cgatatcaaa gcaagccatg gcagcgccca ttgctcccca tgcgatacca
     4861 taacgcgcct gactcaagca acttaacgca gctccaagcc ctttttcact tttcggcaat
     4921 aaattctcgt ctggaacaaa aacattatca aaaaccagct ccccggtaat ggaggctcgt
     4981 aaagacatct ttaacttaat ttcaggacaa ctcatgcctt cagattttgt atcgacaata
     5041 aaaccacgaa ttcccgcctc tgtttttgcc cacactattg cgatgtctgc gataggggca
     5101 ttggtgatcc acattttagc accattcaaa atccagccac cttcaatttt tttggcatga
     5161 gtacgcatgc tggccgggtc agatcctgag tctggctcag ttaatccaaa gcaacctata
     5221 atattccccg cagccatctc tggcaggtag cgtttttttt gctcttcact gccaaattta
     5281 aaaataggat acatacacaa agaactttgc acagaaacaa agctgcgcaa accgctatca
     5341 cctcgctcca attcctgaca aactaaacca taggcaacat aagaagcttc tgcaccccca
     5401 tattccgcag gtaaagttaa gcccaacaaa ccaagatcag cagatttttt tatcaactct
     5461 tttggaaaat gggcatgttc ataagattca gccattaaag gtattacgtc attgctaaca
     5521 aaacgagaca cactatctcg tatcatgcgc tcgtcatcat gtaattgttc atcaagaaac
     5581 aacaagtcgt ccactttcct tctccctttt atctgactgt aatgcgatta ccgcgtctat
     5641 aatactttcc tgacttggca ataaatactg ccaagcagtt cctaatggta tgaagcaatc
     5701 tttgcccgta atccgcttta tttttaaccg tggcgaagcc tcttccatca ataatgtcat
     5761 caatccttca ctaattgagc cgctttgtct accctcatca acaattaaaa tacgttttgc
     5821 tttggcaatt tcttttaaaa tggcgtcttt tggcaagggg ctgagccagc gcaaatcaat
     5881 gatcttaaca gaaatattat gtgcttcacg caagaccttt tccgcctggc gagataaata
     5941 atatccattg gcatatgtta aaataacagt atccccctcc ccatatactc caacctctcc
     6001 ttgagaaatc atttcatcag gtgacgggta ttcaaataac cacccattat ctcccgggct
     6061 atacaaatcc ttggtcatgt agagggcgat gggttctaaa aacacgacaa cccgcccttc
     6121 ctcataggca aggcgcatac atgttctcaa catttttgct gcgtccgggc cattagaggg
     6181 gcaagccact ataacccctg gcaaatcgcg taaaacggct atcgaattat cgttatgaaa
     6241 atgaccgcca aatcccttct gataagcaag agaggcgatt cgaagtacca tagggttttg
     6301 atattggcca cttgaaaaaa aagataacgt ggatgcttct ccacgtaatt gatcttcggc
     6361 attatgcaga taagctaaga actgaatttc cggaacagga ataaagccat tatgtgccaa
     6421 accaatagca gtacctataa tggtcgtttc atcgagaagg gtatcaaaaa ccctgcgttg
     6481 cccaaaacga gcctgcaaat cagctgtaac tcgataaact ccacctttct ttcctacgtc
     6541 ttctccaaaa atcaacatat taggatactg catcatcaga tcggtcaaag caaaattgat
     6601 ctgctggcat aaattacgct ttagggtgag ttgattataa gctccggcaa atacttgcgc
     6661 tcgtctatta tcatctggca atgagtattt ttgcttttta tcaattcgtg ggacaattga
     6721 agccattatt tcttcagcag aactcattct gggttctctg attgcctcca gtgcttttgc
     6781 ttcaattaaa gcccgattgt cctgataaag ctcaaccatt gcctgcaaac tcatccaacc
     6841 ctctctgtac aaaattcctg ctgtatgcaa taaagggtcg tttgcttctc gttgctcaat
     6901 ttcttcctga gtgttgtatt gcgattcaat atcagaaccg gcatggccca acaaacggac
     6961 acaacgcatg tgcaagaaca cgggttgttt ttttactctt gccaaatagt ccgcttcctg
     7021 cgctttggca aaaacatcag caatatttaa tccatcacac tcaatgtaat ggattcctgg
     7081 acgagaggag acggatgact taatccaact ggatggagta ggtactgaaa taccgattcc
     7141 attatcttca caaatgaaca caatcggtag cggataattt tgctgggtta tccaagagca
     7201 agcattcaat gtggcttggc tcgttgcatg gttggttgaa gcatcaccaa aggaacaaag
     7261 tatcacggaa tcaggcgtca gtttacttgg caatcctaac tctctcgccc gggtaattga
     7321 taaagcggcc cctaaagctt ttggtaaatg agatgcgata gtcgacgttt gcggcgggat
     7381 atttaaaggg acgctgccaa agactttatg cctgccccct gagatgggat cacttgccgc
     7441 agccactaat gacaacaaga tatcctttac tccatcgatt ccaggtaatt gctttgctcg
     7501 ttgcaaaaaa aaggcaccgc tgcgatagtg caaaaacgcc atatcactcg ctctaaaaac
     7561 ctgacccaaa accgcattgc cttcatgacc actacttcct atcgtataaa aagagaggcc
     7621 tttttccttt aattgtctcg caataatatc aagaagccgg gatttaattt gtgagtcaaa
     7681 aaattctata gcagttttct tgtccaactc tgctgtatcg gggctcatgc tgcttcttaa
     7741 ttgtgggaaa tccccttgca acactctttt cagaaattgt tcatccacta cacttgctct
     7801 atctaacatc accacaccct gtttaaattg cgcaatgacc agatgaataa ggctatttca
     7861 attacatttt gtgtaatgat tactcttata tccatgcctg gtatcctttc ccaacgaact
     7921 ataaagaaat tcataattct gccgataatt aagacgatga gtatatactt attagacaac
     7981 ttacgaaaca gtccagcagg ggacgacgat aagaaaaagc agtctaactg ataataagga
     8041 tggtgtatgt cggtcgagat gagcaaaacc tccagtgtca gattccctcc tgggcaagaa
     8101 atcaaattag caaaaccggc gacagaactt tatgtaggac aaattctgaa aacagttgtt
     8161 gtcacaacac tgtctaatga tcaggttctt atcaatatca atgggcaaaa tctcaacgca
     8221 aaagcatctc atcattttac accgggagag ttatttgaag taaaagtctt agcgaaccac
     8281 cccgaaacca tccttgaagt gcaacaaaaa acaccgcttc catctctatt gcaaaatgcg
     8341 ttacttcagt atttaccaaa acaagcacca gctaccaatc ttttacagac gctgactcaa
     8401 ttgatggcag aaaataagtt atcggctccc atcaatcagg ttatgaaaac cattttaggc
     8461 aatgtccctg ttgtgactca attacctaat caacttatgc aagcgattag tcaaagtggt
     8521 gtgttcttag aatccaaatt gttaaaagga aagatacccc gagaaataca atcggatttt
     8581 aaagggttgt gttttaaatt attacactgt ttaggcacag acagtaaact cccaatcaat
     8641 ctgccgaacg aattggaatc aggttttctt cagcaagcgc cccttccttt accaggagca
     8701 atccctcagc cactgactaa agacacaata ttgaatctta ttggactatc aacagaggcg
     8761 gtacaccata ttttacgtga gcaagtaaca caggcattag cgcgaatcac aacaaatcaa
     8821 attaattatt tatcgcagga gaatcaagaa acctatctaa tcatgcttga ttttccttta
     8881 aaaacccccg atggcattga tgtaatccct attatgatca aacaacataa ggcagaacct
     8941 atgcaactgt ctaaatggtc aatatcgttc gctttgaatt tatcccattt aggaagcata
     9001 caagctacgg tttcgattta cgctaaaaac atcgatatca aggttaatac tcagcaatcg
     9061 tcaacattgc aactcatcaa tgattaccat aaggatttgg aagaaacgct ctatgaatcg
     9121 ggacttcact taagagaatg gaagctgcaa gttgggttgg aagaaaatca tatcgatgcc
     9181 acaaaccttc gtttattgga tataaaaata tgaaaagaaa gaaacccgtc gctgttgcat
     9241 tacgctatga tggcaattct cctcccaaag taaccgctaa aggagaaggt ttatttgctc
     9301 agcaaattat caatacagct aaagaacatg gcattcctct cgaacaaaat cccgaattaa
     9361 cagcgctctt atctcaagtt catctcaatg aagaaatacc caaagcgctt tatattgccg
     9421 tagcccaaat tttggcattc ctttattaca tcaatggaaa gcactccaaa gaataaatcc
     9481 atttcaaacg aatttgaact tcttcagata aacaaacgcg caaaggcatc cataaaacat
     9541 tggcaatatt tgaacaaata atctacaatt tggttaagtt atgacgagag gtttcgctat
     9601 gaaatggata atgctgatcg ttgttattat aacaactgca ggttgctgtt gtactacagc
     9661 agaagtagtt gaataccaac aagtgacagt tgccgctcca gtggttgagc ccgtcatttt
     9721 agatgtcgat gatccggagc ctttggatgt gactaccacc actattgagt tttactagga
     9781 aattatcagg cttcactatt ttctgagaat agtaataaat tctatttttc agtccagtct
     9841 ttaagtgaag ccctacgcaa tcactcaatg tatgctaaat ttgtgtcaaa aaaacttatt
     9901 aatcttacgt taatgtttgc cagctataat aaaataaatg ttataatatg gaataaacca
     9961 tgccacgaat cactgaaaat atagaaacct atgtcacgca tttattaagt gatctggaac
    10021 ccgttccttc tcaaaatcag catcttgcct acggttatcc cggcgaaaga caggtattca
    10081 aagacccgat actggatggg aaacaagtcg tggtagtgaa cagtcaatat gataaacatg
    10141 gcagacctgt tacagggcag cctgatgtga tccaagaggc gaataattat atagacaatc
    10201 ttgtagccgc tgccaaatct ctgatagaca aagataagaa aggtgagaag gatttgagaa
    10261 aaaatgccat tgatgaaatg gcaaaaacct tcaaaaagtc catttcagaa actattcctt
    10321 ccgacaaagc aaaagcggat ttgttttcaa gtaaaaaaag ctctgccaat aaagactttc
    10381 tggaacagtt ggccaaagtc gaaggcagtt tacagcaatt cacacaggct gtcgcaaaag
    10441 cctcaggtca taaattaaaa gcactggata aagaaggaca taatattcgc agtcataatc
    10501 gcgatacctt gatacatcgc tttaaagcgc ccaactctaa agaaggcgaa gaacaattta
    10561 ttatgtacat tccgtgtgga aggtacacca aacgccaaca aagactaatg ggcaaggatg
    10621 aatctgaagt caacagagat cacgatacta caagtcataa acgttctgac ctggttctgg
    10681 gcaacagcag tatggcgcgc atgattgccg ggacaaggca ttctgatggc accgtgacta
    10741 ttcaccatga cagtttttct ggtcctggtg cacgcatgcc ctattctgat tttaagggtg
    10801 ctgatgacta taaaaagctg gctatcaaag cggtcacctt aatcaaccag gaagaggtca
    10861 tccaaaccct ggcacagcgt caaattgacc gaatgaccaa tgaagacctt tggaataaaa
    10921 ttcctgaaga gtaccgccct gatgaacttc cccctgatgc agagaaagca cgggcgcaat
    10981 taataaagct gtatgtagaa cataatccat tatctgtcac ggaatgttat acacaagttg
    11041 taacagccgg gcaaagagta gcggccgaaa atcaaaaaga acaatttgaa tacgtacggc
    11101 aaatgatgga tgcttttgat ggctccaaag caaaaatcac cattcaaacc ggctccaata
    11161 cagaagtgga aacagaggtt ggttatcagg cgcgtatgag ttcctgggga gtgaattggt
    11221 ttagacaggt tggagcgctc aatccgctct ctgacaatag tgtcacaaaa aatcaaaatg
    11281 cgcgttttgt aaatcaaatg actgatgacg tcattagcaa tttaggcaaa gtggcacaaa
    11341 acctgggtga ttatgacaaa gcaggcgcat tacacacgtt attaaaaggc ccaaacgtca
    11401 gcgaccttaa tcaacaaatc actgagaaag aaaatgcctt aaaagaggta aaaggagcct
    11461 atcgagaagc attattcagc tattttgaag aatatcagaa aggcgaaggg aaatgggatc
    11521 aagccaaact ggatcaattg aaaaatcagg ttgatggcta tgaaaaaagc attaaaaaac
    11581 aagaatcagc catttatgaa ctgcataatc aaatcgatgc cttaagaaag gcttattaca
    11641 cagaacacaa agggcaaatc aataaggctt tacaagaact taaagaacaa ataagccctg
    11701 taatccagaa taaagaaaca gatcctgaaa ccaaatccag actgcaacac ttttacaatt
    11761 catgtgctta cctcatgcaa gctcaagagc tctattatga gaacacctgg catcatggca
    11821 aaaataattt caagctgcaa accttaatgg cttctctctc ctgcgagctg gattacgcca
    11881 atacgaaagg atgtaagagt aataatgatc gtggccaaag attagcgcaa aaaattgttg
    11941 ggaatgcttt atggacagca atgagtgagg acgggttatt cactggtgaa tttctggatc
    12001 atcgaaggac acatggtaaa gaagttagta aagtagaaca acttgatcgg gaacttacta
    12061 ctatacaggc ccttcatcat actgccaata cgggtgtttc cggcggaaag tttgaaattc
    12121 aggataaggc caactttgct gataatggcc ttttcggtaa ggtcgctaat tttgccaaag
    12181 taaaagaaat ggggcctgaa aacgctttta ccaaaaatat gggtacaaaa attaaagcag
    12241 gtctcgggct tgggttacta gcggctggtg gattagtcgc ccttactttg ctattccctc
    12301 cggcaggctt ggctgtggcc gccttgggaa ttggtgtggc agccggtgcc gccattgttg
    12361 ctggcgttac ttttgcggcc acttatcttt ttggtaccgc tattgatgcc attaataaca
    12421 agatagaaga aggaaagatt aaatccattg ttcgcgaggg tatgaaagtg gcatcagaaa
    12481 attcaccaca acacaaaacc gaacaaaagg atcatttaac ggtgaatgcc aaacttcatc
    12541 aacaattagg tgtgacgcga gaatctgtct tggaaagaca atccactcca aaacaagagt
    12601 ttgaaaaacc caaagatatg acctcgaaaa ttgacttaac accagcgatg gaggatgata
    12661 agcgaagcga aagcaccccc acgccagtaa acttctaagg gttatgagtt taacaatcta
    12721 acaatgaaaa aaatcagcgt tcttattttt ctttatctca taagcacgac cagtttttca
    12781 cttcccaaag gctttgttta tttacacgat gtagcgcctg atatcattga agacataaga
    12841 tatgccaccc ataataattt cataggaaat cctatcccag gttacaaacg cggagtatgt
    12901 atagtcacca gacaaaccgc acaacaatta aaaaaagcgg aagaatacat caaacccaaa
    12961 ggatatacgc ttaaagtata tgattgctac aggccacaaa gagctgtgga ttatttttat
    13021 aagtggagcc gcaatgccaa agataatcga atgaaacctg aattttatcc tcgtgaaata
    13081 aagcaggatt tgtttgaccg tggttatatt gctctaacct ctggccacac gcgaggcagt
    13141 acccttgact taactctggt gaaactaact gcctctcaag aaaaaaaatc taatcatcct
    13201 ttaacacgtt gctacgataa aaagccagat tatcttgatg acgattcgat cgatacagga
    13261 actcgctttg attgtctgga tgtgtcagcc aacattgatt attccaggct gaataaacac
    13321 cagaaagcca atcgcgaatt attacaaaag ctcatgaaac gctttggttt tagaccttat
    13381 tactatgaat ggtggcattt caccctgaaa aatgaacctt atccagacac ctatttcaat
    13441 tttccagtcg aataaatagt taagatcaga actcactctg acacacttat tcatgtcctg
    13501 gatgaaacga ctcaacccaa acctggaaga caattaaaac ccaatatgaa tttatttttt
    13561 ctttggtggg tatattaaat atatattgaa tattatttca tcatcagtta tccttgccag
    13621 ccatttcaag tccatatcac taacggagaa tgatagatgc gtgtttcaaa attacttttt
    13681 ttaactttgg cattgagcgg gaatttattt gctcaagata tcaaagtagt aggaacatta
    13741 aatcagactt taaaagcccc acaaaataaa acactcaaca caaaacccgc catacaacaa
    13801 atcaaattgc ttaaaatgca gttgtcaaat tctgctatca aagcacttgc gagaaaaaca
    13861 gacattacac tcaaaaaacc gaataccata accacatcga cagctctccc atccaaagtg
    13921 gaattaggta tgaataacgt acctgttctg aaccagggaa gctttggaac ttgcgtcact
    13981 tttgcaacga ctgcggccat agatgctgct ttaaacaaag gtgactacat cagccaattg
    14041 tgccaattac aactagggct gtatctggag aaaaatggtc attcaccaag tggttgggat
    14101 ggctcattag ggcgcatagt cttaagtcaa atggaaaatt ttgggatagt cagcaaagaa
    14161 aaacaaaaaa acctgggatg tggtggacta actgaatacc cagacaacga gcaacctacc
    14221 cctgatcatg ccatgtcgct tgagcaattc catgaaatga gcgaagatcc agagactaat
    14281 cctgttattt acacccccat cctggatgtt tatcaggctg tattagacag aacagacacc
    14341 aacaaaacga ttaatgatat aaaaaccgct ttgaatcaaa aagatcgggt tacttttgca
    14401 gtattattgc tggattttga tttgggtgtt atgggtgccg taggaacgaa taaaacacaa
    14461 ttcgatactt gggtactaac ccctgaaatt gcccgtgata tttatcttcg cccatttttt
    14521 ggcggtcatg aaatgattat cactggatat gatgacaatg ccatcgctgt tgatgacaat
    14581 ggccgggagc acaaaggatt atttacttta cgtaactcat ggggtgagca atttggagac
    14641 aagggtaatt tttacatgtc ctatgattac ttcaaggttt taactattga ggcacaacgt
    14701 atccgtgctc ttgctgataa tgaatcagaa gaagagtcaa tgacggcctg aaccaatcac
    14761 catcataaaa tcaatataat cggttaagct aaacttccct catcctggtt cattttgcaa
    14821 gtggattagg tgagggtatt cctaaaccaa acttgattca gctattttac gtcctccgca
    14881 ataaaaattt caccagaaga tcagtccaaa acataccatt taaaaatcat tttgtgtata
    14941 atgtgatcat gttatttttc ttgcaaatat catggcccaa aaaccaacta aaatcacaca
    15001 ccttgtagta ttgggagata gtttatccga tagaggaacg cttaataaac gtgagctgct
    15061 gggttttatc cccatgagtt atttaagtgg attaagaagc aaatctccaa aaggtcgatt
    15121 cactaatggt tttctttggg gggattatgt cggcgcgacc actgcagagc aatttgagat
    15181 agagcatatc cgaagaaaat taagattgca taataatgca agagacaatg cggatatagg
    15241 cgatgaatta ctcatcaatg ataaggcacg aagagaaaat gaaaattctt ttagcttgaa
    15301 tgaagacaat catattctgt ttaaagggga aagatttgct cgcttctatt gcgaaggtgg
    15361 attaacttct cacgactatt cttgtagctt tacctttaac ctggtcctgt tttttacaag
    15421 gctgattctg gccactttgg gaggaaaacg gactcaatta ttggctgatg ataaaaaata
    15481 taatatttcc aatcttgaaa aatctgaaac attagttgtg gagtggtcag gcgcgaacga
    15541 cttgatcacc gccaatgaca agccttctcg tgcggctgca gagaaagccg ttaacgaccg
    15601 aatgcaaaat attgaagcgc ttattcataa taggtacaga aattttgttt tattcaattt
    15661 gcccaatctc tcattgaccc caagatttca aagaaaaaat gcggaagaac aacgtaatgc
    15721 atctgattgt actgaatatt ttaatcagca actgcaggct aaatctcttg agttaatcaa
    15781 caaatacaaa gaattgggaa ttcctgtcaa tctctccgtc tttgatgtta acgagcaatt
    15841 taaggaagtt tacagtaatc ccgaaaaata tggctttgat agagacaagt taaaatcacc
    15901 ctacgttgaa tcggatcttt ttaaagaaaa tcagaaaaac cctattgatc aaaaagaaca
    15961 tatttcacca ggcagaggat atatgttttg ggatgatgtt catccgacta caaaaatgca
    16021 tgactggtta gcagagcgtt tcaaagaaaa atactacgat aaaatgtttc gatttgagcc
    16081 gcctcttcag gcaaagcata ggcacaaaga aactgctgat actctggcaa aaacaacaga
    16141 ctccatggtc aaaaccgata aaccggtaaa gaagcttccg gaaaatatcg tattgatgtt
    16201 aaatgctatc cattcaagag cacaattgat gtgtcaatcc actgatgtgc aacggcgtaa
    16261 aaaaggtgaa ttattaaagc attttatttt tgaaataaaa tgccaacatg gaaaccttga
    16321 aaacatctat gggttaatct ctgcatttac cttaaaccct gaccattcaa aaataataaa
    16381 aacccaccag aatccaatca ttgatttctt tagaaataag gtaacgacaa gaagcgaaga
    16441 tgatattgct gccttgcaaa aagcggtcgc aacgtattta caacctaaag aaagcaatga
    16501 acatcttgtt aaacttacct gataaacaaa atcctaactt aattgatctg gtaagtttta
    16561 atgcatccct gatagggaac acgcgtactt ttcaccccat aaaatagtcc tgcttccaga
    16621 ttattgcaga cttgctctgg acttaaggtg tccaggcact ggggtatcgt tttattttga
    16681 caatttggaa aaatggcgtc aggagtgttc atggcttgat aatgctcatt gccccactca
    16741 tacaaatcgg acgtaaaata agtagtaaaa tttctgaccc tcatttgctt gtctaaatca
    16801 aaaactttca tcccgggatt gttatgatgc gccctgctaa ttccgggagt agagaacgca
    16861 taaatagttg agccatcgga taatttgact ttcctgaatt cctccatatg ggagtgtgag
    16921 aacagtatgg tgatttgatc ataggattga tgatatttgt ctaataaggt caaaaatcta
    16981 tccagataaa tgtcatgcca aaaccggttg ccgttatagg ctgtaccagg aggaacatgc
    17041 atcgccaaca acaattgctt tgcacgatga ttttttaatt gttgttcaag ccaaaataat
    17101 tgtacaaagg catccctttg ttgattaggg tactttggta aaaaaacagg ggtcttggtc
    17161 cattgaaccg aattaagtgc tattaaaata atctctttat tattggggat aacataactg
    17221 gagtaatagc catccttgcg catatgagta tcatcaataa ttaacccatc acaatgagca
    17281 caagcaccat cccaatccag agccatattg agtggcgact tattgtctga agaaaatggc
    17341 tggtaattgc caagcaagga atcattattt cctgtaatat aaaacatggg cttttgaccc
    17401 ggattagctt catacaaacc atgaaataca acttgttcgt attcttcttt tttagatgtt
    17461 gaaaataacg agtgggtagg cagatcgcct aaatgcaaaa taaagtctac cttttgagat
    17521 aattcctcaa atcgtttgaa cgtgacatcc aaaaactcct ttccagtatc ctgcccatcc
    17581 tttgctatat tttctgcacc ataatgaata tcagagattg ttagaaattt tggcgcagaa
    17641 aaagctaaag aaaccaagcc tgtcaacaaa agaacaacac gaataataaa atccaattaa
    17701 cactccaaat caatttatac tgatattcat cagtcctcaa ttatagctct tttacagtca
    17761 ctttgaatac ggtacagccc gaatgcagaa aaaatattct atttgacgat ttatatcgat
    17821 aaggattacg taaaaaccag tcgagatcca gcgtccgttc agactgatga gatgcataag
    17881 catcgtctcg aagtcttggc acgaaatgtg aaatcccata caaggcttcg agacggcata
    17941 aatgcctcct cagcccaaac ggctcgagat aacaactgac cttatcataa cttcacatca
    18001 tttaaatcgt gttttggtca gaacagcaca ttcaaaactc aatccctgat gatgccttca
    18061 gctcaaatca attcaaaatc agttgttcag gatcacagac atgtttgcag cgcgacaata
    18121 aatggataag gattatatga gaaccagtcg atatccaggg tccgttcagg ctgaggagat
    18181 gcataagcat cgcctcgaag ccttgtcacg aaatatgaaa tcccatacaa ggcttcgaga
    18241 cggcataaat gcctcctcag cccaaacggc tcgagataac aactgacctt atcataactt
    18301 cacatcattt aaatcgtgtt tcgggcagaa cagcacattc aaaacccaat ccctgatgat
    18361 gccttcagct caaatcaatt caaaatcagt tgttcaggac cacagatatg tttgcagcgc
    18421 gacaataaat ggataaggat tatatgagaa ccagtcgata tccagggccc gttcaggctg
    18481 aggagatgca taagcatcgt ctcgaagcct tggcacgaaa tgtgaaatcc catacaaggc
    18541 ttcgagactg cataaatgcc tcctcagccc aaacggctcg agataacgct ggaatagccc
    18601 cgcattgcat tttaataatc cgaggctggt ttttctttta attatcaaac tcgggttata
    18661 ccgcttcccg aattatccag gttctctctg ccgtattttc agataaaggt gcttgcaccc
    18721 aaagaagcaa aggttttccc gatggtgtaa caggcatcac tttagaagac aaggctacgg
    18781 cgaatgaata tttcttgcca attgattgta aagctttcca aacgctattc gcattagact
    18841 gattgtctgt cacgattaat tcattgcccc ggtaaaccac atttacatcc tgtcctctca
    18901 agacctgcct cactgccgtt tccaaacgcc tccatttggc ttgatgaact cgctgtaact
    18961 caatttgtgt aggattaaat tggaacttaa tgcgactatg aggagataat acccgtgcca
    19021 gtcgactgct tgttggactt tgctgcaaga aataagtgca taatgcgtct aataatggac
    19081 gtcctggccc agccggtttt ccgggtaatc tattgatata catagcaaaa gccaaagtat
    19141 ggccattagc agtatacaag taaccggata aactgttcat acccgtcatc gtcccggttt
    19201 tggcccgcac aaatccttgt tgatttgggg ttttaaaccg tttttgcaaa gtaccatccc
    19261 gaccagagat gggtaaggcg gcaatgtatt cataagataa tgggaaacgt tgatacaaga
    19321 attttaaaag cgccatggtt tgcgctggag ttaccaaatt ataacgtgat aatccagaac
    19381 catctgtaaa attcgagtct tttaaatcga ttcctgtttg ttgctgcaaa aagtttttaa
    19441 ttactggctg tgcttgcttc caatccacag gactaccctt aattttggca gcagcatgca
    19501 agtacaaact atcagcatat aaattatcag aaggttttaa agtatctgcc atcagttggg
    19561 aaattggctt tgaatagcga gtggcaatta ataaagtacc agcaggcgct cttccaagca
    19621 aaacctgtcc atttaattta atattagact tggcaagctc actcacaatc atcccctggg
    19681 cataaacaaa aggattcttt atagcgatgc gttgctgaac agcccattgc cctaatccaa
    19741 cacagcctct taccgttaaa ttgttctcag gatctaaata aaaccctaca ccacatcctt
    19801 tctcactcgc cttcgtggtc gcttgattat tcaggttaat agtaccgccg ccatcatcta
    19861 cttcaacaat agcaggagcg cctgctttag ccccggggtt gaccgtgatt gttaatcggt
    19921 tggaatcaac catcaaaggt gctattggcg caccataact ataagataaa tcagaagtta
    19981 gccagccagg gggataggcg ggaatggaca tcaggctact gtcaatgatc acattacctt
    20041 gaatggttgt gatattccaa tccttgagtg aagaaagcaa cgtctttaaa tcttctctgc
    20101 taaacgaagg atcgccgctg agatgtaaat acaagtttcc gtgcaaaaca ccttgttgta
    20161 attgattggc gctcgtactg agttgattct tgaattggta gtctggacct aaagccatca
    20221 gagccgctgc ctcggaaaaa agcttcatgt tgctggcagg aatatacaaa cgattggcat
    20281 tgcgtttata tagcgtctcg cccgaggtta gatcggtcac tactatccca aggttcacac
    20341 gagggtttag ccgattaatt aatttatcca cctcgaactg catactagcc gcatgagatg
    20401 acagtgacca tactgcccac atgaatgccc cagtcaacgt ccttttcatt taaatatttt
    20461 ccttaacatt gaatttcaca atataaatca ggttgaattg tttgatacat agtgacaatt
    20521 tctgagttag ttccaataag ctaatcatat ctcaagccca atcgctccaa aagccctgca
    20581 agggtatcat tatcaaaaaa ttttactttt aaccaacctc cgtcttcgcc atcgtttaca
    20641 atttgtacag gagcccccac ttgttcagcc aggatagtct gtaaccgctc aatatctctg
    20701 tcttttctgg gatttttggg actgttcgta tctttatttt tataagattt aatttcttgc
    20761 tctaagtgcc tgacagacca ttcctctcca ataacctggt gagcaaacat ttcttgttgt
    20821 tcaggattta aaccaatcaa tacacgagca tggccaaagg atagcacttt gtctcgaacc
    20881 aactgtttaa ccttatctgt caaggtaagg agacgaagaa tatttgcgac atggctacga
    20941 gatttaccta ccaacgctgc gatctcgtcc tgatgataat ggaattcttc aattaaacgc
    21001 ttataaccag tggcttcctc gattaaattc aaatcctgac gttgaatatt ttcgattaaa
    21061 gtgagagcac atgcctgttg atcactataa tctccaatca aacaagggac ttcatttaat
    21121 cccgccaatt tggccgcacg ccaccttctt tcccctgcaa taatttcata tcgctctttt
    21181 gctatgggtc ttaccaccaa aggttcaatt aaaccctggg aagaaattga ctgagccaat
    21241 tcctgtaact ccgttacatt aaaatcttgc ctgggttgat actgacctgc ttgtaggctt
    21301 gtaacaggta aataagtaaa tcgggtttgc ataggaaaaa atcaagaaac ttaaggatat
    21361 gaaattgttt cacaatagtg cctgaaatgg aattgtatcc ggtttatctg acttcaaaga
    21421 aaaaccctga aacgagaaaa aacttattct aaacagtggg tctgtttatt attttttcct
    21481 cattttcctt gttactttca gattgaactc caaaaaatcg aagagtggac tgataaggta
    21541 cttctttgga tttggatttt aattcttcag cgaagccggg atatttttct aacagtttct
    21601 tgccaagccc tataatatac gaatccttgg agctttgaat gaggcgcata taatgtgcag
    21661 cggtaatcaa gtcatttgca gctccggatt tcaataatat ctcacaagca gataacatag
    21721 cgttcgtgca accttgggca gctgattgca aaatcatctg cttatgctgt gatgatgaag
    21781 gagaataaac ttgtgccaga cgaaataaag ccaaagaatt cgttttagct aatacctgca
    21841 tttcgggcag acgcttcacc acggcttgct ttaatcccgg attatcgggt tctcgttgta
    21901 attgtttaac caaagcagca aattcgctac ttggatctaa ttgcgcggcc atacctcaac
    21961 ctttgattat catcttcatt aaagacagaa taaatgctca aaattaagac aaaattaaga
    22021 gatggcactt ttacattttt tttacacata actgtttttt taacagtatt tttaagggca
    22081 taaatgccaa aaaactccca tcttgatcaa aacgtggaat gaaaattaag ataatttctc
    22141 atacctgtcc caacccaaat gaaatagcat gtgcgctaaa attgctgagg ttagtccata
    22201 ttgccaaaat aaaaaaccaa agagtatgga ctgcgaaaga ctcaataata caaaagagta
    22261 aagaaatcgt ctggatggtg tgcaacctgc tgcaatatag gctggaagtt ggcctacagc
    22321 aaaaataaat gcactcaaaa atattgaaat aattataatt tccgaattaa tttgacttgt
    22381 aaacaataaa gcaaaaaagc acactacatt cattaatccc catctggcaa ttacctcttc
    22441 tacaacccct ccataaagga cacaaccatc aagtcctatt ttggaccgta attccgacat
    22501 aatcttgaaa cttttttcat caagtatgga accaaacaac ccataataca ttatacaaaa
    22561 aataactaac cctaaaaggg catataaaaa agtaggtaat aaaatcactt gaaaggaatc
    22621 caggctggct ttgccttgca aaagaccttc cagaagagga tcgcctagcc cggtacgtga
    22681 agacaggatg gagccagcaa aactcatgac aaatgtcata aataaggttt gcaacacaac
    22741 aaaacgacta aatctttttt ttagtctctc ggtattatcg gctagcagaa aatataccag
    22801 gcgatgcatc acgattaaaa caccaggaat ggataaacaa aacaagacaa taattacagg
    22861 ccaatcaatt atcatttttc atcctttcgc aatacactgt aaaaaaagcc atccatgcca
    22921 tgctccccag gcaatatctg ttgaccatgc ccagtccacc ttccccagga ccatttacca
    22981 tcactcactt gacaatccga atgctctgca acaaatcggg cgatttgcaa ctcattttca
    23041 tcactcataa tagagcatgt ggcataaacc attaaaccac ctggtgcaag cagtggccat
    23101 aatgaattca acatgctgga ttgtacctga gaaattgctg aaatctcttc atccgttcgc
    23161 aataatttga tatcagagtg gcgtctaatt actcctgtag cggaacaggg cgcatccagt
    23221 agaatacgat caaatggatg accatcccac cagcttctgg gttccaatac atcaccttcc
    23281 accaccgttg cttgcaaatc caatcgattt aagttatctc tgacccgctg taagcgctgt
    23341 gggtctaaat ccaatgcaaa acaggaagcc aaaccagact ctttctccaa aatatgacat
    23401 gtttttccgc caggagcaca acaggcatcc agtactcgct gcccgggttt taaagacaat
    23461 aaatacgctg ctaattgagg cgcggcatcc tgaacagaca cagaaccttg cgcaaaacca
    23521 ggaagcgtca tcacatcaca agggattgcc aaagtaattc catcaggtgc gacagggtgt
    23581 ttaaaagcct ctatcccgga tttttttagt aaatcaagat actcatcaac tgagtgtttc
    23641 aacacattga ctctcaaagt catgggtggg tgcctatcat tcgcttcagc gatgaattgc
    23701 caatcgtttg gccagtcttt ttgcaggcgt tgaagtaacc aattcggctg tccatagata
    23761 aaaaccggtt ctgctgccaa cttttccaga atatggtctt gtcgacggca aaaattcctt
    23821 agcacggcat ttaccagtcc ttttgcccaa ttttttttaa cctgccccag taaagacact
    23881 gtctccttca ccacggcata atccggctta ttcatatact gcaattgata aatacctatc
    23941 aacaagacta tccatacctc attatcttta ggtcgcttat tgattaaagt atcggctatg
    24001 gattgtaaac gaaaataatg acgacaaaat ccatagcaaa tttcttttgt catgggagaa
    24061 atctcttgcg gagacatcaa ataagacagg ttttttctgc tttctatcaa ggccgtcaaa
    24121 atttttaagg cgttaaatct gtcatttact ttcattgcaa cattaaacca gtatgcaatt
    24181 gtgttttacc cgaatttaac caatccctga ctgaaatgac ctttgctccg ggaaattgaa
    24241 tcttttccac taacaatgca ttatcactgg tagcgaccaa cattccagtt ttatcgatat
    24301 ttaataccat gcccggcatc tcggtactca tgatatcagt tgcttttgcc tggtgaattc
    24361 tgagcattaa ttccccaacc agcgtatagg caacaggcca tggattaaat gcgcgaattt
    24421 ttctatctat ttccactgca gactgatgcc agttaattcg tgcctcttct ttattaattt
    24481 taccagcata agtcgctaac tcattgtttt gtaattcaaa ttgtgctgaa ttggatgcta
    24541 aagcctccaa aacatccaat aatggcttta ctgacatttt tgctaattta tcatgcaaac
    24601 taccagcggt atccgaggaa gtcacaggac aggcggcctt gcataacata ggacctgtat
    24661 ccaatccaac atccatttgc ataatggtca ctccggattc agcatcacca tgtaaaatgg
    24721 catgttgtat tggagatgcg ccacgccagc gcggtaacag agaagcatgc acattaatac
    24781 aacccagacg aggaatttcc aacactgctt ttggtaatat taatccatac gctatcacga
    24841 ccattacatc gggctttaag gcagaaagtt catcgacagc ttcttgattt ttaaaattaa
    24901 gcggttgata aactgatatc tggtgattta ttgcccattc ttttacagga gactcctgta
    24961 atttacggcc tcggccagcc ggcctgtcgg gctgagtata aacagccttg agatgatgcc
    25021 ttgattgaat taaagcatcc agacagggta agccaaattc tggagttcca gcaaaaacga
    25081 cggttaaacc attcatggct tacgagcttg taaacgcttg aacttatcta acttccggcg
    25141 agccatcatt cgcttcaatg gagacaacat atcgacaaac aatttgccat tcatatggtc
    25201 aatttcatgt tgtaaacact cggcaagcag cccttcaccc gtaatttcaa acggtttgcc
    25261 gaacctgtcc agtgccttca cggtcacttt ttcagctctg acaacggtat cataagcccc
    25321 tgggacagac aaacagcctt cttcaaattc tttttcgcca tgagaggaaa ctatttcagg
    25381 attaacaata acgatctgct cctttttatc tcccactata tcaatgacag ataaacgcaa
    25441 gctgactcct atttgtggtg cggcaagccc aactccccga gcatcataca ttgtatcaaa
    25501 catatcgttg attaatgtct gtaaactctc atcaaaagtc tcgaccggct tagctatttt
    25561 tcttaaccgc tcatcaggta aataaagaat cttgcgaatt gccattgttt atctagttct
    25621 ccacacgtta atcgtgatat tatccccgat atacagtaat gctgcaagat gctaaggctc
    25681 aactaaaggg attgatacat gagatattgt ctcctcattt tctgtttcat tatatcatcc
    25741 attactcatg ccttaagcct aagaccggat tccccctctc gttatgttgt gcaacctgga
    25801 gacaccttat ggagtatctc cagccgtttt ttgaataacc cctgggaatg gaaagcgtta
    25861 tggcgcgcaa acccccagat tcaaaatccc gacaggttat atcctggtgc cgttctcgct
    25921 ttagagtatt atcaaaacat tccctatctc agggttctgt ctaacggaac aatcaaactt
    25981 tcccccaaca taagagtgac accccaggaa gacgccgttc caccaatacc attaggcgat
    26041 atcaaacctt ttttggatga atcgttaatc cttgatgtta atgtcctaaa ccgagcaccg
    26101 tatgtggtag cactcatgag tgagcggatg cttggaggcc agggagatga aatttatgtc
    26161 agaggcttgc atccctccaa agaaatgcct caaggaggca ctataggata ctccatcttc
    26221 aggggaggga gaaattattt tgatccaata acccatgaat tgttaggcta taaagcagtc
    26281 ttggtcggtt atggagaatt ggttgctggc ggcgagcccg ctacggtttt attgaccagt
    26341 attaatcagg gcatcaaaat caatgataag gtattgatta ataaccaccc tgaatttgaa
    26401 ttgtatttcg aacccgaaac gcctgccagg caagtcagag gatacattat tgaaatgcct
    26461 gacaatatgc ctgttggaaa tacacaagag gctgttggag gagtcatcat cattaatctt
    26521 ggagaaacct ccgggataaa accgggagat gttttggcta tctatggcaa ggaacgtatt
    26581 gtcaatgatc cccaaaatca tctcagacct attaccttac cgcaagaacg tcttggcgaa
    26641 gctatggtgt ttcgtgtgtt tacaaaagca agttatgccc tgatagtaag gtccacgcgt
    26701 gctattcatt tattagatac agtaacgaac ccatgaataa tctgcaaccc ctcctcgcct
    26761 taaacaggat gaaaaaaatt ggcccccgaa ccgtattaaa actgcaaaaa agatggcctg
    26821 atttaaacct tatgttccaa ctgtccagtg ttgatctgga agaagcgggc ttgccatcct
    26881 ggttggcaca aaccataaaa aattttgatc cggactttat aaaaactgac ttggactggt
    26941 taagttcatc agaaaatcat catattttaa cttgggactc cccctattat cccgcattgc
    27001 tcaaagaaat agccgatcct cccttcattt tatacgctaa aggtaagctg tccgctttga
    27061 cacagcccaa cctggctatt gttggaagtc gcaatgcttc tgtaacagga aacgagaatg
    27121 cccgagcatt ctccagggaa atttccaggc atggggtaag catagtgagt ggtttagcct
    27181 tgggaattga tgctcatgct catttaggtt gtctggatga aggcggcaaa acgatagcgg
    27241 tcttaggtac cggaattgat tgtatctatc cgcgccggca tttaaaatta gctgagcaaa
    27301 ttacagagaa tggtttatta ttaagcgaat ttcctttaaa aagtccacca attgctggac
    27361 attttccact cagaaatcgt ataataagcg gtctatcatt atgcattttg gttatcgagg
    27421 cagcaattaa aagcggttct ttaattacag caagaatggc tctggaacaa aacagagatg
    27481 tattggctat tcccggatcg attcataacc cgttagccag gggctgccac tacttattgc
    27541 aacagggtgc gaaattagta acttcagtgg ctgatgttct tgatgagcta aaaattgagc
    27601 accatcagtt cacttctaat aaaccaattt tttcccttgc cagcggtaag gaaaacctag
    27661 taaagttcat tggttttgaa actacgacta tcgatcaaat cattgatcgt agcggatatg
    27721 gcatggaaca agtcacgagt ggactggctg aactagaatt gaaaggggct gtgatggcag
    27781 tgcccggtgg ctacataagg tgtgaatatg aaagataatt tatttgaaat gttattgaat
    27841 ttgtttgaaa aaagtcttac tcaactccaa aaaagccata aatccaatga tcaggactcc
    27901 caaagtcaat tgcttgaaga agatctttta agtgctgaag agcaatcatt gtttatcaaa
    27961 tctgctcaag acaaatcaac acgtattttt acttacgatg agcaaatgaa actgaccaaa
    28021 gccagctatc aatttctaat gcgtatgaaa ctatggaata tccttgatat cgatcaattt
    28081 gaatcaataa taaatcaact acaattctct gaatctcgta ttgtaacctt gcaagaaaca
    28141 aaatggacta taagaaatgc tctggcatcc accttgaatg aagagcaatt aacttttctt
    28201 gatcttgtgc tataccaaac agaagatgaa ttaacaattc actaattaat gagtgtactg
    28261 atatcaaccc attcatgaac tgttagtgag ccacacaacc caaaaggata aggtttattt
    28321 gatatgagta aacacttggt gatcgtagag tcgcccgcga aagctaaaac aatacagaaa
    28381 tatttaggaa atgactatga cgtgttagcg tcttatggtc atgtgcgtga tctgcctgct
    28441 cgtaaagggt cagtaaaccc tgaaaaacat ttttccatga cttatgttcc tattgaaaaa
    28501 aatgcgagac acattgatac gatagcaaaa actcttaaaa aatcagactc tctactgctg
    28561 gcaactgacc ctgatagaga gggagaagct atctcgtggc atatttttga attaatgaaa
    28621 gaacgaaacc ttttaaagga caaatcagtt catcgaatct tttttaatga aattacaaaa
    28681 gcagcaatcc aggatgcaat taatcatcct cgctccatat ccatggattt ggtgaatgct
    28741 caacaagccc gacgcgctct tgattatctg gttggcttta acctgtcccc tttgctatgg
    28801 aaaaagattc gccgaggatt atcggccggg cgagtacaaa gccctgccct taggttaatt
    28861 gttgagcgag aagaggaaat tgaacgtttc attgctcagg aatactggaa aataatcgca
    28921 aaatgcgctc atgccagcac tgaatttgaa gcacgactga cccattataa cgaagagaaa
    28981 cttcaacagt tttcagtcac tcaacaagaa caagcccatg aaataaaaaa gcaactgata
    29041 gcacaggcac aaggcttttt aaccgttgct caaattgata aaaaacagcg taaacgtaaa
    29101 ccatcccccc cctttattac ctctaccttg caacaggaag ccgccagaaa attaggtttc
    29161 accgcacgaa aaactatgat ggtcgctcag caattatatg aaggtattga tataggcaca
    29221 ggaaccgtgg gtttaatcac ttatatgcgt actgactcag tcaacctagc aaaggaagca
    29281 attgacgaaa tacgcgacta tatcacacaa cgttataagg gagataattg cccgaattct
    29341 cccagaattt acaaaactaa atccaaaaac gcacaggaag cacatgaagc gattagacca
    29401 acttccatca aaagaactcc agaaatggtg caaggttccc tgacaagcga tcaattgaaa
    29461 ctctatagct taatatggaa acgcacagtg gcctcacaga tggctgatgc catcctggat
    29521 acggtgagcg tggattttag ctgtggtaaa ggaaatacct tcagagccaa tggttcaaca
    29581 atagcctttc ctggcttcct ttctgtctat gaagaaggac gagatgattc taaagacgaa
    29641 gacaatgaag ataaaatttt acctgctttc aatgtaggtg aaaaaataaa agtatcagat
    29701 attgaaacca atcagcattt taccgagcca cctccgagat attctgaagc aactttggtt
    29761 aaagctcttg aagaatacga tataggcaga ccttctactt atgcctctat cattcatact
    29821 ttgcaacagc gcgaatacgt ggtagttgaa aaaaaacgtt tcttgcccac tgacgtgggt
    29881 agaattgtca atcgatttct aaccaactat tttactcgct atgtcgacta tcagtttacc
    29941 gctggccttg aagacacact tgatgccatc gcgcgtggag aaaaggattg gatccctgtt
    30001 ctggaagagt tctggcaacc tttcgtccag caaattcaga atatagatga acaagtgcaa
    30061 agaaaggatg ttactacgga attattagat gaaaaatgtc ctaaatgtca aaaaccattg
    30121 tccattaggc tcggcaagag agggcgcttt attggctgca caggatatcc ggattgcgat
    30181 tacactcagg atataagcaa tccagaagga gaaaaatcag aacctgaagt cgtggaaggc
    30241 agaagctgcc ctctgtgtca tggagcctta catattaaaa ccggtcgcta tggaaaattt
    30301 attggatgca gcaattatcc tgaatgcaag catatggaac ctttagaaaa accctccgac
    30361 actggcgtca catgccctaa gtgcagccag gcaaaaattc tgcagcggaa atcaagaaaa
    30421 gggaaaatct tttattcctg tggaaattac ccaaaatgcg attacgctct gtggaacgaa
    30481 cccgttgatt tgccttgccc taaatgtaaa tggccaatct taaccttaaa agaaagtaaa
    30541 aaatttggtc gtcaaattct ttgtcctcgt gaaggttgtg attactctgc caaggaagac
    30601 taggtacttt gttaccccgg ggcctacgcc caagcctttc agactattgg atagcgaata
    30661 atatgtcttt tttattcgtt atccattaat ccagcaaatt atctcaactc accaagggat
    30721 tgttattttt ctgtaagtat aatttggcca aatgatagga agcataatcg gtgatatgtc
    30781 ctgaatcgcg aaatactgga ataccattga tatctacctt gcacatcccc gacgggcact
    30841 gagctactcg tgggtcaata atgaccagtg tcttgtattt tctttgcatt tttgcaaaca
    30901 aattatcgaa ccaaatctgt tcactgacat ccaggttaaa atcacaccgc tcgggattgt
    30961 attttttatg cagcttgata tgctcaaaaa aacaattgta aggatttccc ttagacagtg
    31021 ctatcgattt gattaataca ggtttcgctc ctgaagtggt aataagctcc agagctttat
    31081 ccaaagcctt ttcaatgcgc tttaaagaaa gctcttgaga ccgattgtca ttcaattgat
    31141 taataattct atcaccaaaa taaccattcc agttttggcc taaaataaca tagtcatagt
    31201 ggttggtttt aatcatatta aaatagcgat tcgtttgatc atggcattcc tggtaaatct
    31261 tatttttaac attccaatca aattgatata tgtcaggcaa agcaagacaa gcaaccgttg
    31321 catgagccaa aatggataca ttggcctcct ttgccaacgt atccataaat ccccaatgct
    31381 gattggaata agaatcgcct atcataaagc cggttcgact atccgagttt tttgctccca
    31441 gaacgcactg atggttgact tcggtgtttt tgcgttccag acacaaaggc ctctgtggat
    31501 ttatgtactt gtttaattca gcataaacct ggattgcttc attgaaacga tgaggataac
    31561 ccgcattctt tttaatcaga taatcactca agtgagtgac aaaaaccggc ataatcaata
    31621 aacaaaccac tgtatagctg aaccggattc gatgcaattt tcttgctggt ttttcaataa
    31681 atcgccagga aagataagcg ataatcgata tcaacccaaa agtaaccaac agaacaaaat
    31741 tggactcttc aatgtttaaa tagcgcatta aagccaatac aggccaatgc caaatgtaca
    31801 aggaatagga aattaatcct atgaacacca gaggtcttaa tgaaagagca taagttacta
    31861 taggttgcgg ctcttgttgt ccggcggcaa tcagtatggc cgttgcaagg cataaaatta
    31921 aagtatacca attgggaaaa cctatattaa tgtcatgatg cattgcaata taaaacagcg
    31981 tcagaacagc caagacagtt ataaaattta atacatactt attggctaaa aaacgtttac
    32041 tccccgaagc tgaagcaaca caggaaccaa ttaaaaactc aaagatacga ctggaaaatt
    32101 gataatacgt ttttgcagga ttactccaag agtaatacat ggataatgcg aaaaaagtga
    32161 tcgttaataa atagattatc ttgattcgat gctgtggacc aatcaatcgg taaagcaaat
    32221 agattccaac tggcaagata aaataacatt gccattctac ggataaagac cacatatgta
    32281 aaagaggcaa ttgattgcta tccggagaaa aataatcgct cgtgacattt ttaaaaaaca
    32341 cattcgatgt aaaaatagat gttttacggg cacttttact aaaaagcatc aagtcatcgg
    32401 gcagataata aaacagggta atcaaggttg tgactacaat caaacaaata aaaacaggct
    32461 gtaatctcca taatcgcctg ctgtaaaagt ccataaaaga aaaatgatta ttttgcaaag
    32521 atttttcaat gatacttgta atgagaaatc ctgaaatgac aaagaaaaca tcaactccaa
    32581 taaacccgga tgggaaaaga gttaagcctc catgaaaaaa taaaacaaat aaaatagcaa
    32641 ttgctcttaa gccatcaata tctgagcgat agttcacact acaatttcca taaaatgatt
    32701 gttgattaat actaattaca tgcccaccct tgtactttat ttctgtacaa aggtttgcaa
    32761 attgggaaat tcagagttat atcacaataa tcttttttta gtgagaacta ttttagtgtg
    32821 gttcattcat caattgttca atgtagcgtc tgcaggttaa cggtttatat aaataattaa
    32881 ttaaatagcg agttccacca gttcctgtga ttatcaaagt accataactt aaaatactgc
    32941 ctaaaatgga ttgacgtatg tctatcgttt caattttgtt cagggggata tccacagttt
    33001 gccttactat gataccggta cgtaaaataa cttgcttcct ttttatggta atggaagaaa
    33061 aatgataggt tacccaggta atcaatatcc agatcagtgc aaaaaatcct aaaaaaagac
    33121 tcacttcgcg caactggatg atataaatat aaattgccat cactaaaccc aatccgacga
    33181 aaggaccaaa aaatatgatc caatgcaatc tggtaaaata aatgacatta ctttctgtag
    33241 tattttcagc cattgatcac ccttatatac ctcaaaaaaa tatgatattg cacttaacgt
    33301 taatttgcta ttctatacat aaatcaactt acttttcgat agtctaactc tttaatagat
    33361 acctaacaat taagacggaa gttatgaaat caatcagtca atgcctaaac aagcaactca
    33421 gtacaatttg ccagcattct atgcaattgg aagagttatc tcttaaaatt tctgcatttc
    33481 ttccttccca tttcatttct aaatgtcaag taggtagttt taataaaggg tgtttggttt
    33541 taaccacaac caatgctgct tttgcgtccg aaatacgtta tttacttcct gaattacggg
    33601 ataaattgag aaaagaagcc ggcctttatc agctgtcttc aattaaaatt accatcattg
    33661 ctccctcctc tcaaatcgaa aactctgcac aaaaaaaatc tcttaccctg tctgaaaaag
    33721 ccaaggcaac cattattagt gagagccaac actgtactta tgagccactg aaaaaggcat
    33781 tactgcattt agctcaaagt gaataactca tcaaccatct catttgtatt ctgaattttt
    33841 tttcactata atgctcaaat attcaactaa tatgttagag cttgctatgc ctaaaataaa
    33901 taaaattgtt aatggaacag acctaacccc ccattatctg agtgagccaa acaaagaatt
    33961 taaaatctat cgtcataata acgaagttta tgctgttcgt tttgataatg atgaacccat
    34021 ggattatgtt ttaatgtgga aaagtcacaa gagtgagcat acacagaaca gtgaaataat
    34081 taaaaatacc gaagaggatt ataaggagct tggaaaagga gaacaaggaa cggtatatga
    34141 gaaaacagaa gataaggcta tgaaagtatc tcgaggcaaa catccaagag aattttatga
    34201 agaaattaac ctccatatta tagaacagca atttttcctt aaatatcatg gcattcaaga
    34261 gcactttgtt ctaggactct ggaatatcaa aaatgaagaa aacgtgtatt tctacatgcc
    34321 taaaatcaat gcgatcccta ttaataaaaa aatcgaccag cccaaaattg aggaatttgt
    34381 tcttgcgctt aaagaactta atgatgcagg gtactggcat cctgatttgg caaataatcc
    34441 ttatcatatt agtccacaaa atctgattgc aacagaagaa atggtaaaga ctattgatct
    34501 tgatggtggc tttcggtacg acaagggaag agttgatgaa ctcagcagga agtctcttgt
    34561 ttatggtaaa gatcaatggc tatatgtcta taatttcatc tatccaccca ccgatgagga
    34621 ggataacaga attgattgga gagtccctgt agagaaatgg tatgaaaata acagagacga
    34681 atctttatcg gataatcccc ataccctgct tcgattttac catgagggac ttatatcatt
    34741 gccaaaaaaa cttgtccatg atctgcatga gacaatatta gaagagttat cccaggataa
    34801 aaaatttaaa aaacattcgc atgaggtagg ggtgaatcgc ttttttagcc cgaaaaaaca
    34861 agaaatctat gagggtttaa agggtgatgg gctaaaaaaa gtgatcctga aagaacttag
    34921 agattcgttg gctgagataa gaacaatgga gcaattggag gaaaaaaaac tggagttttt
    34981 agcttcccca gaaatgcaaa ttcttgctga aggtcaggat aagacaacaa aagcactgaa
    35041 cttaaaaacc agttctcgta aaaaggtgat ggtaatattt aaagaagcag aagagcgaat
    35101 actgaatagc cctaatgtaa gcatttagga cgtgtccatt ttaaattcta tgaatagcat
    35161 gcttttatgt gagttagttt cggcttaatt caaataactc ttgaaaaatt aattaagcct
    35221 ttttcatggc aaacttatag cgtttcgaca atttttttaa gtaaagctcc attgataact
    35281 tccccataaa aatggagcct cactatttcc ttctttcgct aacatttctt tgaggcaatt
    35341 cgctcatcat cgatattggc ttccaatcga ttaaggtgca ttataatcaa attaatacaa
    35401 tatatttaaa tccttcttaa ggtgatcatg ctgaaacaaa ttgtgctaac cggcctagtt
    35461 attataggga ttgtcattac gctaatgtac ttatttcagc gtcacctcat ttatttccct
    35521 aatagacata ccccaaagtt ggaagattac aatgcatcgg atatgaaagt agtttcctta
    35581 cgtacaaaag ataatttaca tttaaaatcc tggtataaac ctgcaagcaa gcatcgccct
    35641 acaatactct atttgcatgg gaatgcaggg catattggtt atagaatgcc attggttcgt
    35701 gaatttattg acgctggttt aggagtattt ttattagaat atcgtggtta tggaggcaat
    35761 ccgggcaaac ctggcgagaa aggtctttat gaagatggag aagcagccat tgaattttta
    35821 attcaacatg gtgtgccatc aaagcgcgtg attctatatg gggaatccat aggtactggc
    35881 gtagcaactc accttgccac aaaatacctg gtttgtgcgg ttatacttca atcacccttt
    35941 acatcactaa ccaggcttgc ccagtatcac tacccattga acttccttaa accctgggat
    36001 caatataatt cacttgcccg tatgaaaaaa attaatgccc ccattttggt tttgcatgga
    36061 aagttggatc agatagtacc gtatcaagag ggcttaaatg tttttaatga agccaacgaa
    36121 cctaaaaaaa tgatttcttt tgacgacaaa gaacataatg atttgtggag cgctgataat
    36181 ttttcgaggg aaattattca atttgcttta aaccagtgtt cctagctttt gtggatagtt
    36241 cattattaac gcattttcat catcggcgtt attattaaac tcaatcatcc atttccgcaa
    36301 aggccattga aaatctcctt tcaacccact ccttattgag gtactgaaag caatcttgca
    36361 gataatcggc caattcttct tcatccaaca catgctcaaa acctacaggt tcaacatcat
    36421 gatgaaagta ttcgccgaag tacttcattg aaaaatcaat ataatcccga gtatgcaaaa
    36481 taaaggaatg ccacatctca tcgaggatca ataatggccc aaaaagatag gtttttttcc
    36541 cccgcaacct tctttgttta ttaagccaca tccaggctaa taaatcctca aagagaaata
    36601 gcccttcttg tatggaatat tctggatgag aatagcaaaa atgggtcact attttttcat
    36661 ttttatagtg aagtaattca gaaagagaaa aactcattaa cgtccacaac aagttttttt
    36721 attacttggt ttctgagtaa tgaatctaat tattctacaa atcatttctt ctttagccat
    36781 aattgtctct tattgttaaa atcattcaag tttaacccaa tgtttgtaaa gggcgcaact
    36841 gaatgttttt attttcatca atcagtatca tatgttgtgg tggcacatct tcccatcctt
    36901 catccaaatc attcagcctt tcagaagaga ctacaacata agctggatta ccttcttctt
    36961 ttatccataa tccttgagta gataatacat aaccaatcaa ataatgcagc gtcaaaggtt
    37021 ttttcttctc atgactgcaa tagcgggtag cgataatctt tttaccattt gtaagacaaa
    37081 tattaaaata ggaaggtcca ctatgaccaa attgtcctat tacttccaat atcttgttaa
    37141 tagtttcttg caacacatca gctgccaccg atagttgatt aatatctctt ccttttgcta
    37201 attgcaaaaa caaaccaaac aaatgctcag agtctgtttg gcctttaacc caatggtaaa
    37261 tatcatcatc caacaaatgc cttatatgcc ttttcacatc aataaaatca ttaatttcgc
    37321 cattatgcat taacatccaa tcaccataaa taaagggatg gcagttgtaa ttggttactc
    37381 ctcctgcact ggcagagcga acatgagcaa aaaaacaagg agactgaatt tttgctgtta
    37441 agtgtaaaag atttctatcg ttccaagctg gataaacaga agtaaacaaa gcaggatcgg
    37501 agctaacttc tggtgaatac catcccaaac caaaaccatc tccattggtt cgatatttgg
    37561 tttcgcgcgc atgcagactc tgcatgatga ttgaattctt aggtcttacc aacacatttt
    37621 ccaatagcgt agggcgcccc aaataagcaa cgaatcgaca cataacagcc tccctcatcg
    37681 agtgattcga atagcaaact ctaattaatt aactaacttc caactttcta ttactcatat
    37741 ccctaaatct gtaatccatt cggctgtcat tgtgcgtttt tataaggtac ccggaatcac
    37801 tgcaaatcaa ctcactttac catatgatgc aacgattctt acttatcata gggctaccat
    37861 ttggaatatt aaatgcctct tgaaattgag gaatatttgc caagctacca ttgactctat
    37921 atacagcagg cggatgtgga tctgttgtca cttgattacg aagttgctcc ggcctgacat
    37981 tcatagccca aacatgagct gttcctaaga aaaattgttg atcaggggta attccttcta
    38041 tcgttttagc attcttgtac tcttttgaac gctgaaacgc tttatatgcc aaaatcattc
    38101 cgcccagatc tgcagctgct tcgccaacaa ccaattgtcc ctgtacatgc agatcgccat
    38161 ctactacata ttttgagaat tgatcagcaa tgcattgtgt cgcatttttg aattttgata
    38221 aatcatttgg cgcccaccaa ttttttaaat ttccataacc atcaaattga gcaccttgat
    38281 catcaaatcc atgcgtcatt tcatgaccta taacataccc tattgctcca taatttatgg
    38341 cagccggagc ctttggatca aaaaagggag attgtaatat acctgcgggt atattgaggt
    38401 tattcattga aggatcataa tacgcgttga tcgtttgagg cgtcatagcc cattcacttc
    38461 tatctacagg tttaccaatt ttgtcgagat ctcgattgat caagaattcg tttgctctga
    38521 taacattcaa tacatagggg ccgcgatcta ttttcaagct ggaataatcc caccatttgg
    38581 tggggtaacc aactcgttct tccattaaat ccagcttttt caatgccgca tcacgagtct
    38641 tgggtgacat ccagcttaaa gtttttatat cttcttgtaa tactgctctg atatttttta
    38701 gtatatccaa gaccttttgt ttggattcag gagaaaaata tttctctaca tacaattctc
    38761 cgattgcaaa tcccaatgca ccgttttcag tgttaactac tcttttccac cttggtaata
    38821 gtttttgtgt gccagttaat gcagaaacca tcttaaaatt ctgatcgaca aacggtttgg
    38881 ataaataagg agcgaatgca tctatcaaat gccaacgcag ataaattttc cattcattca
    38941 aactaactga ttgtaataat tcattcatcg ctttgaaaaa atcaggcata gcaaaattga
    39001 tttttttaat tttgccctga cctctagcgc tcaagtattg agaccaggaa aaattaggtg
    39061 tcaatttatc caattcatta atggtcatca tatgataaat ggcgtgtgga tctcgttgct
    39121 ccacttgtga catggacgct ttagccaatt gagtttcaat atccattaca attttggctt
    39181 cttttgccgc catttcagga ctgtcaccca atagctcaaa cattttagta agatggttaa
    39241 cataagcttc tcttacctgt ttaaatttag cttccttttt aagataataa tctctgtctg
    39301 ggagtcccaa tcccccttgc ataacagcac ctatcatgga agtgctgtcc ttaaaatctt
    39361 gcatgctgcc aaaattaaac aagggcccta cgcctatttt gtgcagatgg gcaatttcag
    39421 cctgtaaatc tttgagattc ttaatttctt ctattttagt gaattctggt tttagaggcg
    39481 tgatacctga cttattaatg ctatcaacat ccataccgct aaaataaaaa tcccccacct
    39541 tctgctcaat acttccaggt ttagcgtgag tattcttgga tgcattgatt aacatctgat
    39601 gaataatatt ttgtactttc tcatttacaa cataaaaact tccccaggaa gcatagtcag
    39661 cgggtattgg attattcttt ttccaattgc cattggcata ggtataaaaa tccacagctg
    39721 gtgatacact ggtatcacgc caatcaagat gaagaagatc gtttgtatta ttatcggaaa
    39781 aattggagtt ggaagaaaat actataaaac tggataacat cgtaacaata caagttgcaa
    39841 tcttaaattt catcctgata ttccccttaa tttgagcttt acataaattt ctaaagaagg
    39901 aaactgctta ggccattata tttagtatag ataatgccta taaaatgata atagtgaaaa
    39961 tgaatattct cactacgtcg atatgatgta tttctgtcag gcttcaacag gataatattc
    40021 agggtgaacc gcgggcagat agttttctgt atcaaaatag gtatattccc aagcatcctg
    40081 ccttatcatt aattcacgca acaatctgtt gttcaattcg tgaccagatt tatacccttc
    40141 aaatgcgcca attaaactgg aacccaataa ataaagatca ccgatagcat ctaatacttt
    40201 atgagtaaca aattctgatt cgaagcggag accatcttca ttaaggacac ggtaatcatc
    40261 tactacaatg gcattatcta gactaccgcc ttttgccagg tcgcactctc tcaatttttc
    40321 ataatctgaa agaaaaccaa aagttctcgc tctacaaact tctttgacat acgatgttcc
    40381 agaaaaatca aagcttactg tttgtggtct atcattaaaa accgggtgct caaaacctat
    40441 ggtaaatgtt attttatatc ctttatgagg aaggaactga acatactttc cattttcttc
    40501 gactcgtata ggtttaagaa ttctgatata tctttttgct gcattttgct ctcttatgcc
    40561 cgctgattga attaaaaata caaatggggc agcacttcca tccataattg gaatctctgg
    40621 ggcattgaca tctatatatg cgttatcaat tcctaaaccg gccagggcag ataataaatg
    40681 ttccaccgtt gcaattttca ccttgccatg atgaagggtg gtgcaaagca tggtatctcc
    40741 gacgtattcg taggaggcag gtatttcgac aactggtgac aaatccacgc gtctaaaaac
    40801 gatacctgtg tttacaggag ctggtcttaa ggttaaaagc actttctcac cggaatgtaa
    40861 tccaacacca gtagcctgga ttaccttttt aggagttctt tgttttatca ttcagtgctc
    40921 acctttctct cttatattta ttttgcacgc aaaaacctta agatcttagc aacaatttat
    40981 tgattagtct aattttttta atttcaatta atgcagtttt agaaaccaaa tacaaaaatt
    41041 gcaaaaaata accacacgaa tcaattcatg agatactcaa gaaagaacgt ttgtatccat
    41101 caaattcaaa cacatcctct ctcaagatat cccgttaaaa atgcttctat gcttcttctt
    41161 gcctacgtaa aaacgccggt atgtccaggt aatccacatc aggaacattc tcattaccat
    41221 tattggtttg ctttgtagct gttgttccag taccagtctg agcctgctta cgaatcaccg
    41281 ccggtctctc caattgttga taatcaagag acccatcgct tcttgtcgtt tcaaccaggc
    41341 gtgctctaag aggttgttgt ggctgttgct gacgctgtct attatcacct aaaccagtca
    41401 caatcacagt tactctcatc tcgtcagtca tttcaggatc aataacagtt ccaacaacta
    41461 cagtggcatc atcagaaatg aattctttaa ccacgtcacc cacttcttca aattcaccaa
    41521 tagacatatc aaggcctgcg gtgatattca ctaagattcc gcgagcgccg gagaaattca
    41581 catcctccaa taaaggagat gcaatcgctg cttcggccgc ttgacgtgcc ctttgctcac
    41641 caactgcgct tcctgttccc atcatggcca ttcccatttc tgacatcacc gtacgcacat
    41701 ccgcaaaatc aacgttgatc aaaccagggc gagtaattaa atcagaaata cctttcacag
    41761 caccaagcaa cacattattt gcagctttga acgcattgag caaactaata ttttttccta
    41821 aaacacttaa taatttatta ttaggaatag taattaacga atccacgtgc tcagccaagc
    41881 gtcgaatacc ctcctcagca gcaagcgccc tttgttttcc ttcaaaagaa aatggtttag
    41941 ttaccacagc gaccgttaaa atacctaatt ccttagcaat ttcagcaaaa acaggcgcag
    42001 ctcctgtccc tgttcctccg cccattccgg ctgtaataaa gaccatatct gcaccactaa
    42061 gaatctcttt aatgtgctct ctgtcttctt ctgctgcttc tcttccaatt tgtggattag
    42121 cacctgcccc caagcccttg gtcaatgcat cacccaattg aatgtgtatt ttggcactgg
    42181 aacctcttaa agcttgtgca tcggtattcg cacaaataaa ttccacacca tcaatatttt
    42241 cagcgaccat atgttcgaca gcgttaccac cgccaccacc aacacctaca accttgatga
    42301 cagcattatt ttcttgctgg ctgctttcca ttaattcaaa cataaccctg tctcccctaa
    42361 tgtgctgctt ttttcttatt aaataaatta aacagttttg aacttatcgc atttgacact
    42421 ttacaagaca aaaatctgca tttgttgtca tgcaaaatat catgcaaccc catatctcaa
    42481 tttcaatcca taccaggaac atttttaagc atcatatcag tgtttttaca tgaaaagcta
    42541 aaaatttcct tgaaaccatt ctttcatgcg tgaccaaatg cttttaccat tatcattcat
    42601 tgctggcaca ttatagccac tttcatattg ctgttgaaag ccatgaagca gtaaaccaac
    42661 ccctgttgca aacgaaggat tttctgtcgc ttctgtcaaa ccagagacat aatgcgcaca
    42721 tccttttctg actggcattt caaaacaaag ttcggccaac tcaattgctc ctcttacatt
    42781 ggaagctcca cctgttagaa caataccagc agccaaacgg tcttcaaacc cgcttcggcg
    42841 taattcatta cgtaccagtg aaaatagttc ctcgtatcga gctgaaacga catctgccaa
    42901 agctttggcc gagatttttc gcccaggcct atcattgaca ctggtaactt ctaacatttg
    42961 gtttggattg gctaattctg gtaatgcaca agcatgattg accttaattg cttctgccgc
    43021 cttggttggc gtacgcaagg ccattgcaat atcatttgtc acttgatcgc ctgcaatcgg
    43081 tatgactgag gtatgctgga tagctccctc acaaaatatc gcaatgtcag tagtgccgcc
    43141 acctatgtca attaaacaaa ccccaagctc tttttcatcc tcagtaagaa cagcgtggct
    43201 tgaagctaat tgctccagaa taatatcaat cacatccaaa ccacagcgcc tgacacactt
    43261 cacaatattt tgggcagcac tgacagaacc agtaacaata tgaacccgag attccagcct
    43321 tacaccagcc atgcctatcg gttcacgaat actgccttga tgatcaatga taaattcttg
    43381 cggtaatata tgaagtattt tttgatcagc agggattgcc acggcctttg ctgcatcgat
    43441 aaccctttcc acatcggctt gagatacttc ctgatcacgg atggccacaa taccatggga
    43501 attgaggcta cgtatatggc tgccagcaat gcctgcgtaa acagtcctca cctcacaacc
    43561 agccattaat tcggcttctt gtacagcacg ttggattgaa ttgactgtgg tttctatatc
    43621 aactacaact cctcgcttta atccgcgaga gggatgacgg ccaataccta taatttctat
    43681 cgttccatct gatgttactt caccaattaa tgcaataatt tttgaagttc caatatccaa
    43741 ccctgtgata atattttttt ctattttttt agccattatc gtcccatttg ttgtttccac
    43801 tgcactgcca tgccacgtgg ataacgcagg tccacgccag ccaactgatc tgctttttca
    43861 gcaaaaacag cagggtatgc tttacaaaaa cgcagcaacc gctcttctaa ctctttcttt
    43921 cccaagtata tttttacact atgattaagc agcaacaccc aggattgatt atctcttaaa
    43981 tacaagcctg aagcatttaa accgtatgat gataatatct tactcaattt ttcataaact
    44041 tgtaagactt ctaattgctg agattgcgga ccctttaatt tgggaatatc caaatcagat
    44101 ggaacagaac cctgattgaa caactttcca tcccttgtca ttagagcatc accccaaatc
    44161 gctacaggct ttttttcagt gagtttaatt ttcaatgtat ctggccaaac tctttctaca
    44221 taggccgtat caatccaact cattgaattc aactcacttt gcaatccttt tactgataat
    44281 aaaaagaaac tggcatccag atatttagct aacacattct ccaattcttt atgtgttata
    44341 tgttcatacg tcgccgcaac tttgatcgtt gtaattggaa aacgctctgc atcagcaaga
    44401 tataagtagc ctaagcgacc cgctagcagc aaagcactaa gaattaacaa acccattatc
    44461 gttaaataac gcaagccgac agagttcact tccataattt agtgttccct tcacaagtaa
    44521 tttgtcacca aatgttgacg acagttatga tgaatcattg ctagattaat caattcatca
    44581 agtaaatcag gataagctat accagttgcc tgccacaatt tggggtacat acttatggag
    44641 gtaaatccag gtaatgtatt tatttcatta aagtaaattt cttctgtctt atcatttaca
    44701 aaaaaatcta ccctggccat gcctttgcat ttaagataac taaaaatatt cgctgcagcc
    44761 tgttttaatt gttcttccag actattattt aattgagctg gaataatcaa atccgtttga
    44821 ctgctttcca aatatttcgc agtataagaa taataaccgt cagggtggtt tacttttatc
    44881 tcacccacca tacttactct tggtttccca caggggatac tgttctccaa cactgccagt
    44941 tcaatttccc ttccgacaat aaattcttca accagaattt cttcatcata gcgtaatgca
    45001 tcagcaactg ctgcgtttag ctcatccata ttatttgctt tgtgaattcc cacactggaa
    45061 cctagtgagc atggcttaac gaacagaggc caaccaaact ccgaggcgac ttcatgacaa
    45121 aattgttgtc tttcagacgc attggcatgc catgataata atttatatct tgctgatttg
    45181 agcccattaa tacatgcgag tcgcctcgcc atgtctttgt ccattccgat tgcagaagac
    45241 aatacgtcac atcctacata ggcgacaccg gctaactcaa gtaaaccttg taaacatcca
    45301 tcttcatata aagggccatg aaccactgga aaaacaattt cagcatcaac tgccagacgt
    45361 ccattaatta ataagccttc caatggggtt gatttttcag taacaacagg taatttatca
    45421 gaacaggcga gcaaatcatt ataatcatga cgatgaaatc gaccgctttt atccattgca
    45481 atgggtataa tatgatattt ctcggaatcc aggtgtttta gaaccgatgc ggcagaaacc
    45541 aatgacactt catgctcacc tgattttcca ccataaagta aaaccaaatt aacgggtttg
    45601 gacattagcg tctccaatga tatgtacttc gcgtatcaac tctatagatg tttgttctcg
    45661 aactttagtt tgcaccaaat gaatcaaggc ttcaatatta gctgcggtgg cagtaccttg
    45721 atgattgata ataaaattgg catgtttttc agaaacaact gccccaccaa tacttacccc
    45781 cttgaggcca caagattcaa taagccgcgc tgcaaaatct ccaggagggt ttctaaatac
    45841 cgacccacaa ttatattcat tagttggttg ggtcttggct ctatgagcca ataaatcctt
    45901 aattaattgc aaggatgtct ctttattacc tggtgatagc tgaagctttg cggaaataaa
    45961 ccattcatct cccaagccag caacatgtcg ataagctact tcaaattctt ccggtttacg
    46021 ggttctgatt tcacctcgac gatttatcgt ttgtacttca ataaccgact gccaggtttc
    46081 acctccatga caaccggcat tcattcgcag agcaccaccc atggtaccgg ggataccagc
    46141 ccaaaactct cctcctgaca gattattacg cgcagaaaaa cgagccatac tggcacagga
    46201 tacccctgcc tcaacccgaa tacagttatc gctcaatagg gtcatttctt ttaaacagcc
    46261 ctgagtgaga atcacggtac cggaaaaacc gccatcacga attagagaat tagagcctaa
    46321 acctaaccat aataatggct catcaaaagg caatcgactt aaaaacaaag ccaaatcatc
    46381 aatatttgct ggtttataga gcctcgctgc agggccacca actcgccagg tagtatactc
    46441 ggccaaaggc tcattgaaaa gcaaagtgcc ctgagactct gttacatgac tgctgtctat
    46501 tcccgttatg ctcatgttca ttctcacatg tttttcatca ggttcactgc catttggccg
    46561 atacttccag ctccctgcat caaaataaca tcaccatctt taataaattc atctaaggtg
    46621 gctttcaacg attgctcgct cactatggtc actcgtttat ccctggatcg tatttcattg
    46681 gccagatttt cactggtaac gcctggaata gctgtttctc ccgctgcata gatatccatc
    46741 aataacagtt catccgataa acttaaaaca tcgacaaatt gtctatgcaa ggactgtgtt
    46801 ctggtataac gatggggttg aaagacatga accaaacgtc tctcgggcca aacacgtcta
    46861 aaagcatcaa ttgtggataa aatttcctgt ggatgatgtc cataatcatc cacaataatt
    46921 gccgcgcctt tttcgaactg tttttcacca agcatttgaa agcggcgtcc cactccttgg
    46981 aatttttgta agccacgaac aatggaatcg tcatcaactc ctaactcggt ggcaattgct
    47041 atagaagcta atgcatttaa tacattgtga cggcctggat actggaattg tatagtcagt
    47101 tgtttatgtg gtgcaggtcg aacgaccaca aactcactca gcatgccctt ttgagtccaa
    47161 ttaattgccc gataatgagc ctcttcttta aacccataag ttaaggttgg tctttgaata
    47221 gccgggagga tacggcaaat ttcttcatct tccaaacaaa ctactgctaa tccataaaaa
    47281 ggcaaatgat gcaaaaattc caaaaatgtt gttcttaatt tttcaaaatc accttcataa
    47341 gtatccatgt gatcagcatc aatattagtc actaccgcca tcataggctt caaaaacaaa
    47401 aacgaggcgt cgctctcatc agcctctaca acaaaataag ctgactttcc aagctgagca
    47461 ttcgcaccac aactattcag ctttccgcca ataacaaaac tgggatccaa accaccttca
    47521 gcaagcaaac tactgaccaa gctcgttgtt gtcgttttgc catgagtgcc agcaattgct
    47581 ataccatgcc taaatcgcat tagctcagca agcatagctg ctctgggaat aactggaatc
    47641 attaactcac gagcagccac aatttcggga ttattaaaat caacggccga agaccttact
    47701 acaacatcag cgcctttaat gttttccagc ctgtgaccga tatagacttg aatacctaat
    47761 gaccttaagc gttgtaccgt gccactttcc ccgagatccg aaccagtaat acggtagccc
    47821 tggttatgca atacttcggc aataccacac ataccagcac caccaatacc aacaaaatga
    47881 atttgctcta cacgccccat gcgcggagat aaaaattgct ctgagttatt caatattccc
    47941 ccttaagccg ttagcggtct caacccaaga gactgccaac gattttcatg atctatgcgc
    48001 aataatagtg caataacgat acaattaatt accatactgg ccccaccata actgagtaaa
    48061 ggcagagtta gccctttggt tggtaacagt ccggcattaa cacccatatt aatagaagcc
    48121 tgtaaagcta accaaatagt caaaccatag gctgtatagg atgcaaaatg cctttcttga
    48181 gtataggcgg tatatcctat gtttaatcct ctaataacca gtatactata caatgtgatg
    48241 acaactaaaa ttccaaataa ccctaattct tcagcaatca cagcaaataa aaaatcagta
    48301 tgtgcctcag gtaaatagag taatttctgt atgctttcac ctaatccggt accaaaccat
    48361 ccacctcgtc caaaagcaat caatgactga gtcaattgat aaccactatt gtattgatct
    48421 gcccacggat ccaaaaaggc tgttaagcga gcaaccctat agggggaaga taccgctaac
    48481 agagcaagtg ctgtcactac aaccagcatt aaaccaaaat agtatcttaa tttaactcca
    48541 gctaaaaata acatagccat tactgtgcca gaaatgacaa ctgtcgctcc aaaatctggt
    48601 tcgagcagta ataaaactga aacaacagcc agtattgcca tgggtttaat gaatccaaaa
    48661 atgctttcac aaaccgcctc ttgctggcga accaaatacc cggaaagata gaatatcatt
    48721 gcaagtttcg ttaattctga gacttgcaca ccaatcggac ctaaagctaa ccaacgccga
    48781 ctgccattca ctgatttacc aatacccggg attaaaacaa tgagcaataa aaacacgcaa
    48841 ccaatcatca tgggcatgct tattttttcc cagaaactgc tgtctgtacg cactacaatt
    48901 aaggccagca agagtcccac aaataaataa caagcctgcc gaataagaaa atgaaaaggt
    48961 tgatgaaagt actttgttga aatcataacc gagcttgatg ccaccatcat caaaccaatt
    49021 atcagcaaac caaaaactgc ccctatcagc catttatcat aaagtgatat cggtctgcta
    49081 acgggtttgc ctctttgatt taaatgtctc ggtcgcatcg tgttcacaat ccgcgcactg
    49141 aagatgtaaa cacatcgccc ctatgattaa aatcacggaa catatccaaa ctggcgcaag
    49201 ctggagataa taaaacgaca tcaccaggtt tcgcacatgt cttggcaata gtcacagcgc
    49261 cctccagaga agaagcacgc acgacaggaa ctactttagc taaagccgat tcaatcttgt
    49321 cagcatcttc accaatcaaa acaatggaac gaacaaattc tgaaactggc tgggctaatt
    49381 cttggaaatc cgcccctttt ccctgtccac ccgcaataag aacaattttc ccttgcatag
    49441 aacctcctat tccattaata gcagagattg tagcaccaat atttgttcct ttcgaatcat
    49501 taatccaacc tactccatcc acttctctta cccactgaca tcgatgtggc agtccaggga
    49561 aggtttttaa aacattaaga atgtgttgca tagaaattcc cgctgcttcg gctaaagcac
    49621 aagcagcaag cgcattcatc caattatgca ccccttttat taaaatcgat tccactggca
    49681 aaagacgctc cattcctttc gccaaataaa tagtattctc ttgttcaatc agcccccaat
    49741 ttcccattga aggcgcgtct ttgccaaaag agatgcattt tatatctgct tgacaggatt
    49801 ggtgaggaac agtgtagacg tcttcacgat taaataaaac agccttggca ccacgataaa
    49861 tcctttgttt tgcttgcgta taggcttcca tagtgtgatg cctgtccaaa tgatcaggcg
    49921 ttacattaag aatcgtcgca accactggcg acaaagaata agttaaatcc agttgaaaac
    49981 tggataactc caatacccac aaatcgtagt gatgttcatc atccaacata tctaagacag
    50041 gagtgccgat atttccggct acagctacgc gaaacccggc cgccttagcc atttctccca
    50101 ccaaagtcgt tacagtggat ttaccattag ttcccgttat agcaatgaca ggggcgctaa
    50161 tctctctggc caggcattct atgtctccat aaattgatgc gcccgcttga cgcgctctct
    50221 ctaaaacagg cgtatccagt gccaaaccag ggctagtgat aacatccgtc acctggctga
    50281 ttacttcatc aggagtctgt tgcaggtaaa ttggcacatc aggaaattca ttttggaatt
    50341 cagctactcc aggagcctct ttacgtgtat caaaaacaac aaacgattta ttatttctct
    50401 tcaggtatct tgctatagag agtcccgtct tacccagccc tgctaccaaa tataaggaat
    50461 gattcatgaa tgcaccttgt ctatcgaagt tttaaagtag ccaatccgca taacacaaat
    50521 acaaccgtaa taatccaaaa tcttactatc actttgggct ctgaccaccc ctttaattca
    50581 aaatggtgat gtaatggggc catcctgaat aatctttttc cgcctgaata tttaaaataa
    50641 cccacttgta atatgacaga aagcgtctca attacaaata atccacccat aatgagcaaa
    50701 acaagttctt gtctaactac aaccgcaact atccctaaag ctgcccctaa cgccaaagaa
    50761 cccacatcgc ccatgaaaac ttgagcagga taactgttat accaaagaaa acccaggcca
    50821 gcaccaacaa tagaagaaca aaaaatcgtt agctcaccag tattgggcac ataagggatt
    50881 cctaaataat tagagtatac ggcgttactc gatgcatagg caaacacacc caaggcacca
    50941 gccaccatta ctattggcat aatagccaag ccatccagcc catcagttaa attcactgca
    51001 ttactgcttc caacaatgac gaaataggca agtacaggaa aaaaaacgcc cagatcccaa
    51061 gtgaccgttt taaaaaaagg aactgtgagc tgagtatgta ctggcaaact ggcattccaa
    51121 tacaaataag agacagcaac taaagcaatg actgactgcc aaaaatattt ccaacgacca
    51181 ggtaatcctt tgctattttt taataccaac ttgcgataat catcaaccca accaaccaaa
    51241 ccattagcca aggtaaccaa gagcaccagc cacaggcttg tttgtcttaa atcgcaccaa
    51301 agcaagcaac tcacagtgat ggccaataaa atcaaaacgc cccccatagt gggagttccc
    51361 gcctttgata aatgggtttg gggaccatca ctcctaacca tttggcctat ttgcagccct
    51421 cttaaccagc ggatcattaa tgggccacat aacaaaccca ctataagggc agttaatgag
    51481 gctaaaatag acctgaatgt caaatattga aaaactctaa acgcatgata ttgtccctgc
    51541 aataactgcg ttaaccaata gagcataatt attcctcttg ttcttaaaat tatgctcaat
    51601 gacttttatt gagcataatt cctttaatgg ggcaaaaaag tggccaagca taccacattc
    51661 ctgtcgattc aatgaagagg ttagccctct acctgaacta accggtgcac aacattttcc
    51721 atagcactgg acctggatcc ttttaccaat accgtggtat caggtgttaa atcatttttt
    51781 accacttcaa tcaactcttc ttgactgcaa aaatgctgac ctccctcacc aaacgcctgg
    51841 gatgccagtt cactatgtaa accgcaactc atcaacttat ctattcccag ctggcgagcc
    51901 gctatgccaa cttctctgtg atgttgcgtc gtccattcac ccaactctcc catatcacca
    51961 aatacaaaaa tttttcttcc tgggcgcccg gataaaactt ccagggctgt taagacagag
    52021 cgtaaattag cattgtatgt atcatcaata actgttgatt gattttgccc tgacaacatc
    52081 gtcatccgac cttttacacc actaaagtga gccagtcctc tttgaatgtc attgcctgat
    52141 attcctacag cataacaaca agctgcggca gccaaagcat ttcggacatt gtgcaaacct
    52201 ggtacttgta actcaatctc cactctgcca ttaggtaaaa ccaatgtgaa atgagctcgc
    52261 ccatgggtat ccaagcgtat atcctgggcg taaatgtctg caggatgaag tgaggaaaaa
    52321 cgtaatattt ttttatcagc aatcaaatca tcccaaaagt gagcgtaggc atcatcatca
    52381 ttgactatag caacaccagt cggggataag ccttgatgga tttcaccttt ggaacgagcg
    52441 actccgtcta ttgatccgaa gccttcgaca tgtgccgggg caatattatt aattaaagac
    52501 acatcaggtt gcactatccc taccgtatgc gctatttctc cgggatgatt ggctcccaac
    52561 tcaaaaacag catagcggtg ctgcttattt aaagctaaca tgcttaaagg aacccctatg
    52621 tgattattta aattgccttt cgtagcatgt gatggctgcg gcaaaatagt cgctatcatc
    52681 tccttgacag tggtttttcc atttgaccct gttaaagcaa taatagggca actcatttct
    52741 tgtcgatggg ccgcagcaat tttggccaaa gccattaaag gactgacaac cacaaattga
    52801 ggaatatgaa cgccgtcaat caaacgatta actacagcag ccgaagcgcc tttagcctca
    52861 gcttctttaa taaaatcatg accatcaaag cgttccccca ctatcgcaaa aaataaattt
    52921 cctggcttta atgtgcgggt atctatacta acaccagtaa tgtcggcatt aatctggcaa
    52981 gactgagaaa gaagtgacgc aatagtaatt aggttcatag tattttgtga tgtcgaaaag
    53041 actagtgtac taacactcag gctcacagtt caagatggtt tgagaataga tagataagaa
    53101 tataactaaa aagtcaagaa ttttaagttt attacaatcc gattcataag ttaccggaca
    53161 ttaaccatta acagagtaga taatgagctt cgcctggtgc gaagccctat cctgaaaata
    53221 taaccgttag atagtggaat gattctagcc tattggcgtt agtttgttac acaatcatcc
    53281 atcaaatgct caattttcaa gtatcgatga tatcgttcga tacgctcctc cgttagaggt
    53341 ttgcgctggt catccagcaa ataagcgtcg agcccctcac ttaattgttt tgcagtatct
    53401 agcataacag aaaaccgttc tgcattaatc tcaggattat ttgatatttg catgaacaga
    53461 cataatcctc ttacactgaa agcaccgata ttctgtaaat caaaaacacc ggttgctgta
    53521 gctgcggcta aactgcaaag cactgggcct tgtccattag tgaattgatg tctatgaaac
    53581 aagtgcctct ctccaaatct caaaccagca gccaaaacag tttgcaataa ttcataaccg
    53641 gcaaattgtc gattttcctt ggctaacaaa aacaccatgg cggtggaaga agaacagtca
    53701 ctattaggct ctgttctttc atttgattcg cactcttctt cctcaggaat caactgaggt
    53761 tgctctgttt ttacagaggg cttccttact gcttgtcgct tttcagaacc atcaaaattt
    53821 tcaaaatcca aaggaacctc cctattgacc ttccgcacag cgataatttc atcattatat
    53881 gattgagagc cataggagtt attttctact tggccaagag aaggttgata gtgttcttga
    53941 tttaagctct gtcttctagt cttcattaaa cggccaatag ccactagaac gccaattaac
    54001 aacaggacat taagaattaa gctccaattt gcctgcataa gtttactcca tagtcaaagc
    54061 ttgtttcaca tcaaccgcaa ccaaacgaga cactcctggc tcacgcatgg tcaccccaac
    54121 taaatgttca gccaattcca tagttacctt attatgagta ataaacagga attgtatgaa
    54181 ttgtgacatc tcttttacta aatcgcaaaa ccgacttaca tttaagtcat ctaaaggcgc
    54241 atctacttca tctaacatac aaaatggtga aggatttagc tgaaaaattg caaacaccaa
    54301 agccacagca gtcattgcct tttcaccacc agataataga tgaatagaac tattacgttt
    54361 tccaggcggc tgcgccatta ctacaatccc tgcttctaac agattatcac aagttaattc
    54421 gagctgtgcc cgaccaccgc caaataatct tggaaaaagc gctttaaaag aggtattcac
    54481 ttcatcaaac gtattttcaa gacgcgattt cgtttcatta tccatttttt caattgcagt
    54541 ttccaaagta gataaagctt ggctcaagtc gttatattgc tcatccagga acactttgcg
    54601 ctgctgttcg gcagcaaatt cttcgatggc ggccaggttt atagcgccta gccttttaat
    54661 tttctccaaa atcgctatca actcgtcttc ttttatactt tgcgtgactc cggatggaat
    54721 tcgctctaat aaagactggg cttgtaaatt agcctcatca agagtttctt gaattgaact
    54781 ggcacgaacg gatagggctt gtttttccat ttggatttga ttcatgagtt cctgaatccg
    54841 ttttacttcc tgatcatagt ttaaaattaa tttctcttgg tcttcaagtt gcaacttaag
    54901 ctgagatact ttttctcggc tcaaagtcaa catcaattcg atttcaccat gtttcatcaa
    54961 taatgctgcc aattgatttt taaactcggc ctctggcgct acggactgta aataatgttg
    55021 agttaactcc tctaaacgca tctgcgcacg ttgcaaacgc tctttttctc tacttatcct
    55081 atcggttatt tgctgtatct tggtgacttc tctatcatat tccaactcac tttgatgcag
    55141 caaagcacgg acactctcta cttgtttatt ttgttcaacg agaacactgt tccatgtgtc
    55201 tttttcattt ataaatcgct tgaattgcaa ctcagaagct tggtattgac cttccagttc
    55261 tctgatttta tcagccattt ccaattgttc agcgtttagc tcttccagcg acgactccaa
    55321 ctctattatc tcgttcgtga tagcatcttt ctgtttttca gcgtgaaaaa tagcttgttc
    55381 ctgactattt accgcaatga tatgattgcg tagcgcctca ttactggcat ttaaatttaa
    55441 ttgatgcagt ttgataaaat ttaaattttc ctgtagccgc gcttgataac tatctcttgc
    55501 taaacggatg gattcaattt tttgttgtaa agattccaca cttaaattca actcgataat
    55561 cctttgctgt ctggcaagaa tgcccacctc atcttgctct ttttggcgag gtaatttaat
    55621 ccaccccttg cctagccaaa taccattgac agttacaata gattcatgag ccaataaatc
    55681 aggaaaccag gcctttgcct cttcaagatt gtctgcggca taaatgtgtt caagatggtt
    55741 tactgttgag ggaaccaagc cttgaatttt atcagctaac cttgggcgct cctgcttttc
    55801 cccattgaga gctctcatcg tcaccacgtt ctctttatga tcttcacaaa actgccaccg
    55861 agaccatagc tcagcaaaat catccaatac ataagcgatt agatcatctc ctaatacgat
    55921 ttcacaagaa gattgccatt ggggttcaac ctttaaaaca tccattaatc ttggcttatc
    55981 tgaccattcc gcaagtccgt ccttattagg ccgattttcc ttttttgctg caatttgagc
    56041 agcttttaat gcagcaagct cactgtttaa acgatgaaac tcatcttgca atgcatgcag
    56101 ttgttgctcg gtattttgta attgactctg caaatccgat tgagtctcat tcgcttgcct
    56161 aagctgttcc tcatcaaact cctgtgtttc tttcaattgg aaatgctttt cttgcaattt
    56221 aattttattt ttttgcaaat cgtcaagcga gatagccgat tgctccaaat tcagtttttc
    56281 cagccttacc aggacttgat ggcgcttctg ctctaaatgt tgtagtttgg cttgcgtgag
    56341 ttggtattga tttttaatgg tattggaatt aatttgcacc tcttgccaaa ttttctccca
    56401 ttgcgattgt tccttttgtt tttcttgcca tagagattcc tgttctttga attccatttt
    56461 taaatgacgc aaatgctcct ctaattgttg agccttaagc ttacaattgt gcaattcttc
    56521 attatccagc ttaagttgct cttcaatgag ttgagaatca acctgcattt gctgcctgtc
    56581 attttccaaa cgcttttttt ctctttcttg ttgctgaatg gcttcttcaa gacgagctat
    56641 ctcagttcct gaatgataga gagaatcctg aattgattgg gattgatcaa tggcatcttg
    56701 tacttgttca tttaaaactg cccgctcctt gtaacaacta gttaataaac tttgttgctg
    56761 ctcattgcgg attgctaact cttgtaattc tcgctgtttt accgtttgtt tttctgagta
    56821 ttcctgccat ttaagagcct ggatctcagc tcggcaaatt ttttcctctt cctttaaaat
    56881 ggtatagcgt tctgctgctt tggcctgccg ctccaaacgt tgcaaatgtt tgcccaattc
    56941 ttctctaata tcagcgacgc gcgtcaggtt atcccttgtt tgttcaatac gttgtaaagt
    57001 ttcccggcgg cgctctttat attttgaaac tcctgccgcc tcctccaaat agacccttag
    57061 ctcctcaggt tttgcttcta ttaatcgaga tacagtacct tggccaataa ttgagtaact
    57121 tcgtgcccct gctcctgtgc ctaaaaaaat atcagtaata tcttttcgcc tgcatcgagc
    57181 tccattcaaa taataaaaag attctccatc ccgagtgacc actcgtttta cagcaatttc
    57241 accataactg gcaaaagggc cagtcagtct tcccaagcta ttatcaaata ccagttctac
    57301 tgacgcctgc cctaccggtt tgcgattgga agaaccgtta aagataatat ccgtcatgga
    57361 ctctccacga agatttttgg cagagctttc gcccataacc cagcgaacag catcaataat
    57421 attggacttg ccgcatccat tcggaccaac aacagcgacc aaacgactgg gaaaatgaac
    57481 cgtggtggga tcaacaaatg atttaaaacc agccaatttt aattgcttca aatgcatttt
    57541 aactgcgaat tataaaataa taatcggtta gtatatcgta ggaagtgaat cctgactatt
    57601 tcagaacatc catatcgctc tcttctgcca tcgttaagga caacatgctc aattgacaaa
    57661 caattttatc gctttataat gcatacttct gttaacaaat gtaggtaaac aatgaaacag
    57721 caattctata aattgcccca aattctaata agcctttgtc tcctattgat tctttccacc
    57781 ccactccaag cagaaccgcc aaatcttagt ttaattagaa aagaaattaa aaactattac
    57841 gactctggct tgtattatca cgaattggaa cgaaccatta agttagctca agaatatata
    57901 caccaacaat atttaatcaa taaaaacaac aaacacccac aaaaacttgc catagtactg
    57961 gatattgatg aaaccagcct gtccaactat gataagatgg taaaacgcga ttttaccggt
    58021 agcaaagagc aaattcataa agaaatactt gctgccaatt caccggcaat caagcctatg
    58081 ctgacacttt acaaaaacgc cttaaaacag ggtatcaaag tattttttgt aaccggaagg
    58141 caagaatccg agcgcgacgc aacccgcact aatttaatta aagcaggata tactaaatgg
    58201 gcaggacttt atctacggcc taatgggtat tcttcctcct cgattatccc atttaaatca
    58261 aaagcaaggg aaatgatagc aaaaaaggga tacaccatta ttgccagtat tggtgatcaa
    58321 tacagtgata tccaaggggg gtatactaaa aaaggattta aacttcccaa tccattttac
    58381 tacttgcctt agtcaaggcg tgatgggtga ttaggttttt cgtaggtcct gttgattttg
    58441 aatctatgaa acctcaggca caacgacccg aggtttctta ataactacaa gcattaagct
    58501 gctttttctt tcatccgaat tgccatgact tccatagcca ggaatttgac ataatcatag
    58561 gtcacgtaat aatctccttg atctccagca aatttggacc aagagttacg caaagtgaaa
    58621 acacctttgt taacatggcc ttcttcatcc atcacttcaa gatcatcatc atagcctgtg
    58681 atcactaatt catgcccggc gtaaatcatt ccattcatcg catccagtac aatttcgggt
    58741 gttaacatcc aggtatcatg gtaagcacgg ttggtaccta cagctccggc atccccaaca
    58801 aacacatcca ataacatacc gatagtcaag cgattacctt tggctaactc ctctttgatc
    58861 tgatagacga tttgattcat atccgctttc gctgaaaagg attcctcatc ctttaacaaa
    58921 gcctcccagc taattaaatt tgataccggg acgctatgcg ccaggaactc agagtcagac
    58981 atgggtttcc cttcattatt ttcgtcttct aacggatatt ctctaacacc ggcgcaacca
    59041 ttcaatttct gataattctg actgataata ccgtattcag aaatctgttg aagaacccag
    59101 taaccaaaag aaccattcca cccgctggct ttggctttgt catgtatcgc cagataactt
    59161 cctaactcca gattacacaa ttgactaatg taatcccctg ccccaagggc agcatctatg
    59221 gctgcagtta ctgcgaaagt gacacaacta ccatgatatc cctgatccaa tactggcgtt
    59281 aactgcattc ccaacttaac ttttcttggt aactcagaag aaaaactggc tgtgtttata
    59341 ctatttttag gatattctga cagctgttca cgcaaataag atttcgcttc ggttgataat
    59401 tcatacttgg gcagcttaaa tgttaaactt tgagcgactc cactgggatt tttaggcgta
    59461 atgtggacat caacctctcc ttttaaagtc aaaggacctg caaaagccag cgatgaagag
    59521 aaagacaata cagctaaatt aattaactta ctcataaaat atatccctat aattaaccct
    59581 cttgcatcat atcaagaggt gaattaaatg caaacaaatc atgcgaataa gaataaaatg
    59641 acaagctagc tttctattca cagagtagaa accattttaa aataaggtac ctattcacca
    59701 gagcgccatg ataattttta tcaaacaaat tctatcccga atactgaaat aacatcctct
    59761 cattgacgaa tcaaaaataa caaatcagat tcaatttaag aaaacaagca acttgacttg
    59821 agttcatata taataagcct gattctaaaa aagaggatat taatatgcgt actctattgt
    59881 catgtttcct gataacccta ttgtcttttg gtttaattat caatgaggcc tctgctaaac
    59941 gctttggagg cgggcgcagc ttcggggttc aacgctctca aagcagtctt ttctcatcca
    60001 ataaaacaca caatacttct tcattaacac aaaaagctaa taaaagcaga tggggtggcg
    60061 tattaggagg tcttctggtt ggaggattat tagccagtct ctttatgggt aatggtttgg
    60121 caaatgggtt aatcacctgg ctaatccttg gttcagtcat tttctttgtc gtcagcttct
    60181 tacgcaggaa aatgaaccca gggtttcaag caactcaatc cggagcattc cgtcaaaact
    60241 cttttgaaca attcacgcaa aactacggaa accgaaatag tggaggagct cattttaccc
    60301 aacaagaaac cagctttgac gcagacaatt ttcttcgtga tgccaaggta acttttatac
    60361 gtctacaagc ggcttacgac caaaaaaatc tacaggattt gcaaacattc acagtaccgg
    60421 aagtatttgc agaaattaaa atgcaactgg acgaacgagg cgatgccccc aaccaaacgg
    60481 aagtcatttc cctcgatgca aaattgcttg atgtgtccaa acaatcctta tccatgatag
    60541 ccagcgtaca attcactgga acaatcaaag agaacggtga aatatctaac ctggatgaaa
    60601 tatggcattt ccgccagttt gataacagaa aagagtgggt tgtagggggt attcagcaag
    60661 aagtatttca accataaata cttatggttt cctggagtgg caactgtctt taatgacagt
    60721 tgcactaaaa atcaaagaaa ataactgaaa tcactcaaca atatattgca cctggatgat
    60781 ttcatattca accatgccgc caggtgtttc taccgtgacc gcatcatcaa gctctttgcc
    60841 aattaaagct cgggcaatag gagaactgta tgaaatcttg ttcagtttga tatcagcttc
    60901 atcctctcca actattttat aggttaattc tgagcccgtt gctacatgac aaattgtaac
    60961 ggttgaacca aatatcactt tgccattgtt aggtaattta ctgatatcaa ttatttgcgc
    61021 atgagacaac ttcgcttcaa gctcttgaat gcgcccttca ttaaaacttt gttgttctcg
    61081 agctgcgtga tattccgcat tttcttttaa atcaccatgg gctcttgcag ttgcaattgc
    61141 ttctacaatg cgcggacgat ccacgaactt taagcgatgc aattctgctt ttagtgcttc
    61201 tgcaccctca actgtcatag gatgtttact catatttacc cctaatgtaa atcttgtaag
    61261 cgagttacag tttccctatc ttcatacttc atagccaaac aagcagcttc ggctcctgat
    61321 aaggtcgtag tgtaactgac cttatgctgt aaggcgttgc gtctgatagc gaaagaatct
    61381 gctatcgctt gtttgccctc agtagtattt acaataaagt ctatttcatt attttttata
    61441 aaatccagca catgtggtcg gccttctgct actttaaaga ctcttctgca gtctacgccg
    61501 gctgcttgta atgccaaagc agtaccgcgc gtggcgataa tttcaaaacc cagttctatt
    61561 aatctcttgg caatctcgcc gacacgcgtt ttatctgcgt cacgtacgga tacgaaagct
    61621 cgtcgccgtt tggcaatgtg acatccggca cccaattgtg ctttagcata agcttgtcca
    61681 aaacgcttgg caatacccat cacttctccc gttgatttca tttccggtcc aagaatcgaa
    61741 tccacaccag aaaacttgat aaagggaaaa accggcaact taatcgaata gaaagaaggc
    61801 gtatgatgat tctggtttaa tccttgatct ttcaagctaa ttcctacttt acaaagagcc
    61861 gcaattttag ctaatggcag accggtagcc tttgaaacaa aaggcacggt tctcgaagcg
    61921 cgcggattca cttccaaaac ataaatatca tccgcttgaa tagcaaactg cgcattaatc
    61981 aagcccacca cgcctaattt caaagccatt tttcggatct gctctatcaa atcctgctga
    62041 acaactacgc ccaagctaaa aggaggtaaa gtgcatgccg aatcaccaga atgtatcccc
    62101 gcttgctcga tatgctccat gatggcgcca attaatacgt ctttgccatc acaaacggca
    62161 tcaatatcta cctctatggc atcattcaaa aatttatcaa gcagaacggg tgaatcatta
    62221 gaaacttcaa ctgcatgcga caggtaatac cttaaatcct cttcttgata aacaatttcc
    62281 atagctcgtc caccgagaac ataagaaggc ctgacaacta gcggataacc aattttgtta
    62341 gctaactcaa tggcctctgc ttcgcttcgg acagttccat tatcaggctg atgcagtttc
    62401 aattctttga ccatttgctg aaatcgttct ctgtcttcag ccatatcaat agcatctgat
    62461 gaagtaccaa ttatttttac gccattagcc tctaaatcac gagccaactt taatggtgtt
    62521 tgtcccccat aatgcacaat gacaccctcc ggttgttcga ttgcaacgat ggacaacaca
    62581 tcctctaaag taacaggttc aaaatatagt cgatcggagg tatcaaaatc agtggaaacc
    62641 gtttctggat tgcaattcac cataatagtt tggtaacccg cactcctcaa tgacaaggcc
    62701 gcatgcacgc agcaataatc aaattcaatg ccttggccaa ttcgattagg cccacctcca
    62761 agaatcataa tctttttctt attgctctca ggacgtgctt cacaacttcc ttcataacaa
    62821 gaatacatgt aggctgtatc actagggaat tctccggcac aagaatcaat tctcttgtag
    62881 acaggtaaga tctctaattc aacacgtctt gcccgaactt ctgcttccgt acaaccaagc
    62941 aatttggcca agtaggcgtc agcaaaaccc cggtgtttca tcaagtgtaa agtggtttta
    63001 tccagatccc gaatcgtttt tccgatgatt gagcgctcta aaagaaccaa ctcttgaatt
    63061 tgtgccagaa accatggatc gactcgactt tccagatgaa tttcctcaag tgataaattt
    63121 tgcctaaagg catcagcaat ataccaaagt ctatccggag tgggttctcg taaatgccct
    63181 cttaaacgag ccatatcctc tttatgaaac agcgaatgca atccacatcg tccaatctct
    63241 aaacctctta tggctttctg taaagattcc tgaaagttag aaccaatcgc catgacttcc
    63301 ccgactgatt tcatttgtgt ggtaagagtg tctggagttt gcggaaattt atcaaaatta
    63361 aaccgcggaa ctttggtaac aacgtaatca atgctgggct caaaggacgc aggtgtttta
    63421 cctccggtga tttcgttttt caattcatcc aaggtataac ccacagccaa tttagctgcg
    63481 accttagcaa tgggaaaacc ggttgctttt gacgccaaag ccgagcttcg agatacacgc
    63541 ggattcattt ctacaaccag catgcgcccg tcttcaggat taatagcaaa ctgaacgttg
    63601 gaacctcccg tatccacacc aactgctctt agcactttaa tcgccgcatc ccgcatccgt
    63661 tggtattctt tatcagttaa tgtttgtgcc ggagcaacgg taatggaatc tccagtatgc
    63721 acacccatag ggtcaaaatt ttctatagta caaacaataa tgcaattatc atttttatcc
    63781 cggacgactt ccatttcata ttctttccaa cccagaaccg attcatcaat caaaagctcg
    63841 tgagttggcg acaactccaa tcctctaatg caaatttctt caaattcctc acggttataa
    63901 gcaataccgc ctccactacc acccatggta aatgacggtc tgatgatggc aggaaagcct
    63961 aaacgggctt gcacttgaat tgcttcttcc aggctatgag caatcgccga ccttggcata
    64021 tccaatccga ttttaatcat caactggcga aatttttctc tatcttccgc cctgtctatg
    64081 gcttcacgcg tcgctcctat catttcaaca gagtacttgg ctaatacccc ttctcttacc
    64141 aaatccaagg cgcagtttaa ggctgtttgt cctcccatcg ttggtaaaag agcgtctggc
    64201 ctctctattt cgataatacg agccacttct ttccattgaa caggctctat ataggtggca
    64261 tcagcaagct caggatcagt cattatggtt gctggatttg agttaactaa tatcactcga
    64321 tatccttctt ctttcaaagc gcgtactgct tgggtacctg agtaatcaaa ctcgcaagcc
    64381 tgcccaatca caataggacc tgctccaaga attagaatag attttatatc ggttcgtttt
    64441 ggcataatga caataaaaaa agaataaagt gatatatttt accgagtttt gcccaaagat
    64501 tatacccttg gataacatgt tttgagttat taattgccag aaaacatcca acggcattaa
    64561 ataacttaaa attacttgta taaagtagaa agcaacattt gcaaaccggc ctctgtcaaa
    64621 gacaaatatt tataataggg gtctgccgtt gtgcgctcaa gccattcatt tgcgatgcaa
    64681 atatcggtta atttctgcgc ttcctctact gaaacaggtt tgcccaattt ctgggtcagc
    64741 attgctgctg catcctcttc aatacttaga aaaacctgtt ctctggttac cccacgcgca
    64801 gtcatttcat cataaaacaa agataaaata tccatttcgt tcatagtaat tccttattat
    64861 tagatactaa gatctatagc atatatttaa tcatcgttcg acatgataaa attaaatact
    64921 ttttaactaa aaaattggtc ataaccgaac ctctctatgc aagaaattga tgtccttatc
    64981 attggcgctg gtgctgcagg cctaatgtgt gctattgaag caagcaagcg taaaagaaaa
    65041 gtactcgtgc tggatcatgc caataaagcc gggaaaaaga tactcatgtc aggaggtggt
    65101 cgttgtaatt ttaccaatta ctacattgaa cccaacaagt atttttccca taacccccat
    65161 tttttcaaat cagccttatc tcgttacact caatgggatt ttattgaact ggttaacaaa
    65221 cacaaaattc ccttccacga aaaaacatta ggacaattat tttgcgataa taagtccaaa
    65281 gacatcgttg atatgttgct taaagaatgt gagcaatatg gagtggctat ttatttaaat
    65341 accgtaatag aaaaaataca aaaaaccaac gactattctt tcaagatagg cacgacaaaa
    65401 ggaaaatttc attgtcattc tttggtcatt gcgaccggag gactctctat tcccacaatg
    65461 ggtgccagcc cctttgccta taaagttgca gaacaattcg ctattaaagt atggccaacc
    65521 agagccggcc tggtaccgtt tactttagac gttctcgaaa aagacaggtt atccatcctg
    65581 tcgggaatag gtattgatag cctggtcaat aatgaaagaa atcaatttcg tgaacatatc
    65641 ttgtttaccc accggggact gagtggtcct gctatcttgc aactctcatc ctactggaat
    65701 cctggtgaaa gtatatgcat taatttgctg cccgaacata acctcctgga aagcttgaaa
    65761 atagcccgag cggaagcccc gcataaacag ttaaattctg tattatcgat gtacttgccc
    65821 aaacgagttg ttgaagtctt tattcctcaa aaactgggtg aaaagaaact ggctgacagc
    65881 tctaataaag atttggaaac aatttcccat ttgttgcaga actgggtagt aaaacccaat
    65941 gggacagaag gctaccgtac agcagaagtg acaataggcg gagtagactg tcatgctatt
    66001 tcatccaaaa ccatggaagc caacaatgta ccaggtctat atttcattgg agaagccctg
    66061 gacgtgacag ggtggttagg cgggtataat ttccagtggg catggtcttc cggctgggct
    66121 gccggacaag tagtgtagcg aatttaaaaa ccaagtctca atgcgcatca gcttatctga
    66181 accccaaagc ccatattttt cccaggattt catgaagagc aatggaagta taggccaggt
    66241 tataagaatt aggtatcacc attttgaccc aggtatcagt agaccaaaag aaggtaaaat
    66301 cagttggggc ggctatggga tgcaatcctt gctgctgaca caatgccatg gaacgcggca
    66361 tatgaatagc tgaggtaacc agatagaagg ggtgatcatg cactatggct gctaattcgc
    66421 gtgcctgatc tgctgtattg atagaaacag tttcaatgac aactttttcg ggatcgattg
    66481 caaaccattt ggttaattcc gccaataata aagcctctga ttgttcacca gcagatcctc
    66541 cccccgataa aagcagtttt gcatcaggca aatccctgaa taatcggaca ccttcaacca
    66601 atcgcttgag gcttgctgag gtcaataaat catttgcagg cattccttcg ccaatatttt
    66661 gtcctccacc gagaacgacc acccatttca cattcggatt aacccgcttc actatagggt
    66721 atcgagactc taattgataa gttaaataac tgggtaacca ccccgtactc atagcaagca
    66781 agacaagcaa tacaacaaac aacataactt tagtgaaagt aggagcaagc cgacgatata
    66841 gtaaaaaaat gcaaatacct aatgtcaaaa gacataaaaa gaaaggatgc agcaagtatt
    66901 caagcaaatg tctaatgaat gccattatct gactcttcgc attaaataaa ttccaattaa
    66961 agaaaagatg agagtaggac taaacgcagc caattcaggc ggccattgga atactgtgct
    67021 cactgggcca aaaaatcgat tcagaatatg aaacccgaat ccaaccatag cgcctaccag
    67081 taattttgaa cccatggttg acgaacgtaa ggggccaaaa ataaagggga tagctagtat
    67141 catcataacc atagtattca aaggctgggt aattcgttga aaaaaggcaa acttataatt
    67201 tgctacattt tgatggttgc gttgttgctc acgtaaatag cggttcaatt catgtaaagt
    67261 catttcatcc ggattagtgc ccgtaatttt aagaatttcc ggtttcacag gaacatccca
    67321 cggcaaagta tcgaaatgat aggctttggt atgatcctct gcaaattcag tttgtttcac
    67381 atcataagct atccacgaat tattgacata ctttatttca cttatctggc gagccagttt
    67441 taaattgtgc tggttatcaa agcggaattg atttacatag cgcagagtat tctcgcgcgt
    67501 gactgaagca atagaaacaa aatcattacc attacggacc caaacccctc ttggtgtcag
    67561 caaagcctgc cctcctgtta aagcggaaac cttgtattca ttcgcataat gcgccatgac
    67621 tggtacaacg gtttcaccta aagacgtgat taacaggatg actatcagag acgcctttaa
    67681 taccgccccc gttatttggc cgatggatat ccccgccgca cgcatcacaa ccagctcgct
    67741 atgattagct aatactccca ggccaaccaa acacccgagc aagctggcaa tagggaaaaa
    67801 cagatatact tggtagggca tctgtaaaag aacaaaaaca cctgcctgta cgattccata
    67861 gtctgctcgc cccaaatcgt ctatctgatt aacaaacaaa ataaagatct gtaaaccgac
    67921 cagcatcaaa gtcaccaagc cgattgcaga taaaaccgtt ttggcaatat aacgatcaag
    67981 tatttttctg ctcatgcaag cttcacctga tttcgccaaa tgaataggat gccgagaatg
    68041 gcaaccgcca cgtgtaccca ccacatccct atccaccagg gaatcttccc ggtagcaatc
    68101 gcatcacgag aaacaaacat aaaattagca taaaaaatat agataatgat ggctggcaaa
    68161 agtttcgaat attttccaga acgagggctg acacgactaa gtggaacggc aaccagagtc
    68221 aaggtcaaaa ccattaaagg aatggaaata cgccactgta attcagccgc ctttttcaaa
    68281 tcatgattgt tcaaaggcca taagtctctg gtttttgcag tgcgagcatc atcatgcata
    68341 acaacagtcg ggttgggtaa tttggattta tagcttgcaa attctgcaat ctggtaatcg
    68401 gcatgccccg gaacaccttg atattccttg ccattttgca gcacgatgta ttcctcgcct
    68461 gtttgaggat taacctcagc aaatgcttta tctgcccaca acacatccca taacagctta
    68521 ttatcctgaa cgctatgttt tgccatgaat atctgttctg cctgggaatg atcacgattc
    68581 atggattgga cataaaagat ttgctgaccg ctattaatgg catgaaagcg tccaggcata
    68641 atcgtttgaa ttaaagtttt gattcctgta gaacgtatta acttggtgcg ttcaacagca
    68701 atcacaggac ttgcccaaat cattactatg gcaactacta ccaacacaac agtagccatg
    68761 gcaaagctgt gtttcagcaa ttgcctggga ccataaccgc aagctcgcag aaccgtcatt
    68821 tcgctttcgg catataagcg gccatacgcc aataaaagag cgatataaaa ccccaggggc
    68881 agtaacaggc ccattaaatt tggcaactcc aacatcaaca acttcataat cacaaaacca
    68941 ggaattgaac cagaagcagc gcgatttaag tattgtatga attgattact catgaaaata
    69001 aggactaata tcgcggttaa tgcgattaaa gtaagaaaca cttctttagt caaataacga
    69061 aaaataatca ctacaatacc ctacagagga ccttatcaat taattcaatt ctttcagaat
    69121 aacatatgaa agaacccatc atgataattc aagacagcct atttaaaaat agtattgatt
    69181 taaaacagat gattaaaaca gaatttttca ttcaaatgct tatccaattt aggtaaacta
    69241 gttgtctttc tcaagcggtg caggaaaaaa tattatgaat tatggattaa ctcatacccc
    69301 ctcattaaac atgagcgaat gtctggtgtt aggcgttttt tcagatttgg cgctaccaga
    69361 ttttgcaact gcaattgata atgaacagca agggctgatt aaaaaacttt gccagagagt
    69421 ccctgaacca ggaaatactg tatggcaaac tgatgttgaa ggacatagcc tattaatcat
    69481 tcagtgtggc aaaaaggaag agtttagcgc taattcactc caaaaacgtg taggagaaat
    69541 taccgaagca ttaattaaac agcgtttctc ttctgccacc gtatgcttgc ctcggttaaa
    69601 tcaggaatct tctgaatggc agttagagca aatgattgtt caaatcgata atttacgata
    69661 tcaacttctt gattttaaaa cgaaacatgc aaaaagccat aaattggagt cggtaatatt
    69721 ccatctgcct ggagctactg aaaaaagcct tgaaatggcc aaagccattg ttaccggagt
    69781 tgaattttgc cgtgatttag cgaatatgcc ggccaatatt tgtactccga cttaccttgg
    69841 tgagcaagcc atttcattat ccaaacaatt cgaccaaata agctgtcaag ttatggggcc
    69901 agaagaaata aaagaaatgg ggatgggcgc attattagcc gttgctcagg gaagtgacca
    69961 acccccaaga ttgatagaca ttcattatca cggtaacaaa aactcagcac cggtcattct
    70021 tgtagggaaa ggcattactt ttgactcagg gggcttatct ataaagccgg ccaacgctat
    70081 ggatgaaatg aaatacgata tgtctggcgc tgccagtgtg ctgggagtta taaaagcctg
    70141 tgctttgctg aaattgccaa ttaatcttat tggcattata gccagtgctg aaaatttgat
    70201 aagtggctcc gctgtaaaat caggagacat tgtaaccacc atgtcaggac aaacagtcga
    70261 aattatcaat accgatgcgg agggtcgatt agtgctagca gacgctttga cttacgcgga
    70321 gcgatataat cctgattttg taatcgatat tgctacttta acaggagcga taatcgtcgc
    70381 tttgggcaat atagccactg gctatatgac tagagatgaa cagctggcca aatcaatcga
    70441 acgtgccgct aatgaaagcc aggataaagt ctggcgtatg ccactggatg aggcctatca
    70501 agatgctttg gaaagcccat tggctgatat gatcaacgcc ggctttgatc gcagtgcagg
    70561 aagtattact gccgcctgtt tcttatcacg gtttactgaa aaatatcgtt gggcccattt
    70621 ggacatcgca ggaacagcct ggatttctgg aaagaagcgt aatgcaacag ggcgtcctgt
    70681 tcctttatta attcaattat tgcgccatgt cgccaattcg cgttgatttt tacttattag
    70741 ccagcagcct tccagaatct cgctggttgg ttgcttgtcg tttaatagaa aaagcctata
    70801 ccaaaggaca tactattttt gttttttgcg acaatcagca acaagcagaa taccttgatg
    70861 aattattgtg gactttcaaa gacgacagtt ttattcctca taatttgcaa ggtgaaggac
    70921 cggagcctcc tcctcccgta caaattggct atcaaaaaga acccagaggc tttacagata
    70981 tcctgttgaa tttatccacc aatattccaa ttttttatac ccgatttaaa agagtcattg
    71041 aattggtgag taatgtagaa catgagaaag aagtgagtcg attgcactat aaagaatata
    71101 gaaacaaagg ctgtgcattg cacacacact cgattgaata aatatttttc tgctcgctgg
    71161 atttttaagc ttattgtgta tccgctccct gatggtcgcg actctgatta taataaagta
    71221 aaacagagcc gcgaccatca gggagcggat aaaggataaa gaagcccgga gaatcagcag
    71281 ttacatttta acctggtgat tttgaatgag accacagcac atcacaatcc tttttttcgg
    71341 cggcttgcaa taagtgaatt aaagggattg ctcgtttagc caaactgata tcaggctcac
    71401 tatcctcatc gtcttctagc agtttatttt cgctttttat tttttccagc ccttgttgta
    71461 aatgatgcaa agcatcaggt acattttcag acttaatagc acccgggatt gtgccgccgt
    71521 gccccattaa caacaataat tgttttgcca cattttcaaa ataagtaata ttctcatatg
    71581 catcacaact aaaagtcaca aacataacta gctcccaact gaaaattcta tccgtaagtc
    71641 tgtaataaat actcggcaac cgctcgcaag cccattgctt caccaccttc tggctttcct
    71701 ggcttactta ctgcattcca ggccatgata tcaaaatgaa cccatggtat tgattttgtc
    71761 acaaatcgtt gtagaaataa gccggcagta atagccccag cataggacga agaactggaa
    71821 ttgctcatgt ctgcaatatt cgaattcaat aactcttcgt atggtgaaaa taaaggcaac
    71881 cgccagacgg ggtctgagac tttattagat gccgctacta tatcctgagc cagttgatca
    71941 tgattagaaa acatggctgc aatttcagtt ccgacagcga cacgagcagc acctgttaaa
    72001 gtagcaaaat caatgatcaa atctggtttt tcttcacaag ccatagccaa agcatctgcc
    72061 agaatcaatc gaccttctgc atcagtgttt tctatttcta cggttaatcc gttgcgcatg
    72121 gttaaaacat ctcctggcct aaaagcattc ggaccaatgg cgttttcaac agcggggatt
    72181 aatacctgta atcgaatcgg caaacgctga accattatcc actgggctaa acctatgacg
    72241 tgtgcggcac cgcccatatc ttttttcatc aaacgcatgc ctgatgaagg tttgatatca
    72301 agcccaccac tatcaaaaca aacacccttt ccaatcaaag ttacacgtgg atgtttctca
    72361 tttccccaat gcaaggataa taaacgaggc gttaaagcag aagctcttcc aaccgcatga
    72421 atagcaggaa aattatcctt tatcaattcc tctccaatcc attgcttaaa gtaagccttg
    72481 tgggttttag ccaattcttc gacaacatga gctagcgatt ctggccccat atcgcaggtt
    72541 ggcgtgttaa tgagatcccg cacgagaaat aaagaattcg ccaaagataa aatataatct
    72601 ctatcatttg cctcaacaat taaaactcga ggctgtaact cgtacttttt atactcatca
    72661 aatcgatatt gtgctaatgc ccaaactacg ccagactcac gacttaactt atcttgaaat
    72721 tgatagttgc ctggaggcaa taaaagtgcg gcattggcca aagcactttc ctgattacca
    72781 gttcccgatc caataaaagc tttttcaatc acaccatccg cattgaggat aaaacaataa
    72841 tcgcctaatt tgcccttaaa ctggcgtaca gaaaaacaat tgcgttccat gggggttagg
    72901 ttttcaattc cttcgtccca ttgcgattga gaaatgagat ggacaggaac tatcctatcg
    72961 ccacaacttt tgtaaaatac ttctgcttgc atctttttaa tcctttatct ggccacgaaa
    73021 gtggctaggc ctaagcccta ttatacctaa aactaataaa tagatgacta cagtgacgaa
    73081 cacatgagct aataacaagc tcaaacgcat caagggagag aaaccaagcc agtaagtcgc
    73141 tgttccactc ataaaatata aataaacccc aatggcaaaa ttggctatca acaactgcat
    73201 actgtatttc agccatccgg gagatggttt gaacaatccc cttttaatca agagaaacaa
    73261 caaaatagca caattcacat aaccagctaa tgccgaagcc aacgtcaagc cagcatgagc
    73321 caagtgccag acaaatacaa aacataaaag agtattaact accatggcta tggcgcccac
    73381 ttttacaggg gtgctgatgt cctggcgagc ataaaaaccg gaagccaaca ctttaaccac
    73441 catgaaggca ggaactccca tggcaagagt aatcaggctt ttttgagttt gtaataaatc
    73501 ataggcagta aatttgccat aagcaaaaca actggcaatt aaaggcaagg caaaaaagga
    73561 caatcccaaa ccggctggaa ttcctattaa taaaatcgaa cgcagccccc aatccaaagc
    73621 gcgagaatat tgattaatat tttgttctgt gtgttttctg gaaaggtggg gcaagataac
    73681 cgtcgcaatg gcgaccccaa aaactcccaa aggaaaatca gttagtctgt ctgtatagta
    73741 taaccaagac acactcccta cttttagaaa agaagcaaaa atactatcta ccatcaagtt
    73801 aagttgcgca attgaaacac cgaataaggc cgggatcatt aacttaagta ctttattgac
    73861 tcctggatca tcccgaacaa ctctcggcct gataagcaaa tgtcgttgat acaaaaatgg
    73921 taattgaaaa agcaattgca cgataccggc aatcaacaca ccccatgcca aaccaacaac
    73981 gggttgtggc aaattagggc acaaatacac cgcagcaagt atcatactga tattaagcaa
    74041 aacgggagta aatgctggaa ccccgaaata accataggta tttaatattg ctcctgccat
    74101 tgctgttaag gaaataagca ttaaataggg aaaagtgata cgaagcatct gagttgccaa
    74161 ttcagcccga acgctgtcat gatggaaacc tggcgcgaat aaaaaaataa taacaggcga
    74221 tgctacaatt cccactacag tgaccagagt taatatggaa ctcaaatagc ctgaaatccg
    74281 agcgatgaaa gtacgaacgt cttcagcaga acgcgttttt tgatattcag ccaataccgg
    74341 gacaaaagct tgagaaaaag cgccttccgc aaaaaggcgc cgcataaaat ttggtatcct
    74401 gaaagcaaca aaaaaagcat ccatgccagc ttgagctcca aaaaaattgg caagaaccat
    74461 atctcgcgca aacccgacta tccttgaaat aaatgtcatt aatgaaacaa gggaagtgga
    74521 tcgtatcaag ctttgttttt taggtaccat aatatccgtt gtcagcatga tgttttaaat
    74581 agataaaaat tttctttatg atatacttaa actggcgtga gttcgatata aaaagcatgg
    74641 aaatacctct tatatctggt taagctggca ttatcagtaa aacacaacac atcagtatac
    74701 aggttccact gcaaatgaag tcgatcatgg tatcaaacca gtaagtgaat cacctgaaat
    74761 attggtttta caggatttat ccggataata aaacatttca acatcaacct gggttaaaac
    74821 aaatacataa atacaaaagg ttcaaagcag gtctattgac aaattctaac tcattgtgca
    74881 agatctacat cttttgcaac atctgattga gtggagattt ttaagtggca aatatcaaat
    74941 cagcgattaa gcgtgcacgt caaaacgtca aattgcgtca acataacgcc agcgcgcgtt
    75001 ccatgtaccg cacctacatt aaaaacgtgc tgaaagccgt agagtcagga gatcaagaag
    75061 ctgcgcgtgc tgcttatacc aaagcacagc cagtgataga caaagccgct aataaaggct
    75121 taattcataa aaacaaagca gcacgtatta aaggccgtct ggttgcacgt cttaaagcta
    75181 tggctgccta atcgagagtt tccgtcgctc taagttcgaa actaccgaac cgtaattccc
    75241 ggttcggttc tctagtgtag cccgttggaa taattgcttg cctcgatgag agatttttca
    75301 tcatctgtta tcctgggttc ttggctttga ttccctttga taaaagttga ataaaaataa
    75361 tctctaccca ttgctgctgc aaccccctgg aaaaagccta acatacttaa tggttgagca
    75421 gggaacagca atgcggctaa ttttctttgt gactgcatgt gtatcactat atccaattcc
    75481 gaagacatgg catacacatt ttctctggct attttttgct cattctctac tttttctatt
    75541 ttgccctgta actctttttc caaagaacaa aaaggcgcca tcatcaattt ggcaaattgt
    75601 tctgtaattg cttttaaatg tgagcgattc acttcaatgc tgtcgattgt cgggttggta
    75661 aagccatttg tagtaaagag ccatgtggct acctcattaa gcatgctatg ccaaggtaaa
    75721 gagggtagta aaaattttgt tagccaatac ctgtcgtctt ttacagagga aaaagtggca
    75781 gccaggcact caacaaagaa atttaatgat tcattaaagt acattatcct agcatccaat
    75841 tgctccttat aaatccctaa acactcatga caaaaatccg atgccaattt atattgtttt
    75901 tctgctatct gcagacgctc cgcataatac tctacggttt cacgaccctt ttcgacatcg
    75961 cgccactcct tattgatact gcgtgccatg cgataagcca aatttaagcc tctaaaatga
    76021 acattagttt ggacttcaaa ggttaactcc tccaatgtat gcaattgttc cattaaggta
    76081 gtaagttggc ataatctctt gattacttga ttattttctc ctgagtggca tacataaatc
    76141 actctgggca ctgttcttga gttgaattgc tcgggcaaat tttctgagtg gatcaaagac
    76201 gaggattgct ccagactatg ctgctgaagt tcatcctccg attgattgac caatattgca
    76261 ttgcggatta ggggaaaacc tgtagctttt aattgctcct gcattgcttc ttttgtcgag
    76321 gcttgaaatg cattgagcca attggcctta tcttttgaat tgattgcttc cttttgagta
    76381 tccagccagt ttttggctaa aacatacagt ctctttttag ccaattgaat ttggctttgt
    76441 aaatcattta tggttaaaaa catcctttta tgctcacttg cttgcttgag tgtccttaat
    76501 ctatcactct aaaatgcata cttcaatcaa aaaaattcat tcatagccgg atattttaat
    76561 acaaattgtt tattaataaa caacatggat tatgtaaaaa caaaggtcaa ggcaaaaaac
    76621 atttttaatg aggtggtttt tataaactaa atgactgaat taaaaccatt ttttaacgaa
    76681 gagattaaga gatttcataa atcctttttt atttgatatt aattcctaaa aagggttggt
    76741 ctttatcagt ccctcaacac caacccaagc tggaatcaca aaaagctcat gaactggaat
    76801 ctattccaca acctctacgt tgccaaaggt tgtggagata aagcaatttt cccctcattg
    76861 tcttctagct cgacatgatc ctcgcaatag gtgcgtccga taattttaca aagattaatt
    76921 ttatccttcc ttgataagta agaagctact gcttctacca tattttcaaa tctgttttcg
    76981 ccagtttctt catagggaag gatattttta ttttccattg gagcattatc gtcaatttga
    77041 taattatcca caatggccat ataaatgctg ttaaataacg attttgcttt tttaattttg
    77101 ccttgtaagg ttctttcttc cgcacttctt ggtgctgaaa ataaagaaag cgcccagcca
    77161 taccaacctc caagtttagg ttcttcttgc ttaggctcaa taaaagaaaa agctttataa
    77221 catccaacta ccttattgat taattcatct ttataaggcc gatatttttc aggtaaattg
    77281 ctattgatta ataaccactc caggcgctgc agttttctga caattgcttt cacttcttcg
    77341 caatattccc catctatttg aaataatttg attgtctctt cagataatgc ttcaagttct
    77401 tgcggaggaa ctgtccaata aagctttact ccaggatcct ttaaatcacg atagtgcaca
    77461 acgtctcctg aagatttcct atgctcccgt aaaggacccg tactatgtaa aaaaattgac
    77521 tccattggcc cactacgtct gggcactgtg ggatgttcac catattctgt aaggcgttca
    77581 atttgctcct ttgataatac ataagacagc aatgataaag cttgtgtttt acagttttgt
    77641 gaaagagcta aactaggccc aaacaatccc caggacaaac gcaactcaaa aggttttaag
    77701 cgagcttccc gcccttgttg ttccgcttta attttttcag tcgcataaat caatcttgaa
    77761 aattgttcgt gaggataaat tctggttttt tcaggtggtt tccccttaat gccttgcgtt
    77821 tcgacaaact catttatcgc tgtttcctga tcagttgcca tctgcaaaca ctttttttgt
    77881 aataactcaa aatcgtcttt ggttaactca aaggtaaccc cgcgcaaacc acatccctga
    77941 tcgagaaatc tgagctcttc atgccgcaac attccatgat tgccaatcaa atcaacatcc
    78001 agccccattt ttattttcaa ttgatctaac caagaactat tacgattggt tgtcggtaat
    78061 ccataaaatc cccaattatc aacaacctcc agcaatttac tggtttcgtc catttttgaa
    78121 agcaatagac aactgtgcca cagtggatta ctccctacct cctgatctgt agtacaaaat
    78181 gtaacagcat atttcatatc accaccctca tcgatttgca tctaattatt tgaccttgat
    78241 taaatagacc ttttgcctaa agccgattct ttcattttat tgctgctttg ctaacattcc
    78301 tgcattgctc attaactgca ttataaactc cgctcctcaa aacgtttgca ccttgcccta
    78361 aaaaataatt ggcttaggca taaagcctaa ctaacaattt aaatctctaa aagattattt
    78421 ttaaaattac aataaggcat ggcttcactt tcgtaaatag ccaagatgag cgcaaccctt
    78481 cttgtgttta atataatcga ggcaacaaat gcaggctggg ccttgtggcc cagaaaaatc
    78541 ggataattga ttagcaaaat tgaaacatca tctgttaaat agctcaaaga gccgagcaat
    78601 gatcttaacc caagttattc attaatttgg cttgattcaa ctgctcataa gctcgctttg
    78661 cagggataaa attgggatat tgcgtcacca gacgttgcaa gatcattctg gacaaaacct
    78721 cctccccatg attccattca tttaatgcat caaaataaat catatcagcg ctttggccat
    78781 caaatttggg aacattatta aataacacgg gctcgacata acttacttta ccctgggttt
    78841 gtgcatctaa tctttgcaat actctacggg cctgtaaact tccgttttca ctcgcttgtt
    78901 gtaatagctc acggcctttt tcgggtagtc gttcgcctgc cgttccctca atataataag
    78961 tacctaattg atattgagca taagcatttt gcttttgtgc caatttttga taaatactcg
    79021 ctgatctttt atgatccttt tcgacaccaa gaccataatg atacattctt gccaaagcca
    79081 gcattgcttt ttcattacct ttatcagatg catcctggta ataccgtaaa gcatctggaa
    79141 aatccaattt aacaccaaca ccagtttcgg ataataaacc gagggcatac aaagcattcc
    79201 cattacctaa gcttgcagct ttcttatacc agaccaaagc ttgttgttcg tttcttggtt
    79261 gccccaaacc ataaaaatac attccagcca attgactcat cgcttcagga gcacctttct
    79321 ctgatgcttc tttaaacaaa gataacgctt tggagtaatt gacgggcacg cccttgccat
    79381 actcatacat taaagccaaa ttataagcgc ctagcatatc tccttttgct gcagcatttt
    79441 cataggcttt tagtgcttga gcataattat catctaccgt ttcataaata aaacctaatg
    79501 ctacatcagc tttagataaa tgtttcgctg ctttttgata ccagtgtttg gccaaattat
    79561 aatcaggttc tcctgtttca cctagttgat aaaattggcc taataagtat tgtgcctcag
    79621 gattgccttg ttctgccgaa gcagtatacc aatgttgcgc gccagcaaga tccggtgcag
    79681 cacccaatcc tttttgtagc atataagcca attttaattg tgcatattga tcgcctttat
    79741 cagctaagcc cttataaatt tccttagctt gattcatttg ttcaggaaca ctgctttgag
    79801 ataaataata atctgccaat actatccccg catgactatt ccctaattta taagactcaa
    79861 ttaaattggg taaaaagtct tccccggatt gttgtttaag tactgccaga ttaaagtcgg
    79921 cataggaaaa ttgggagtct gctgattgct tcagcaaatc caatcctttt tccttatcct
    79981 gtgccacccc ttttccttct gtgatgtaag tgcccaatat aaactgactt acgggatttt
    80041 gtccggcttg ttgataccag tacatggctt tggctggatc agcggttatt cctataccac
    80101 ggtcatagag cattccgagt aaaagagcgg cattctcatt gcctgtttca gcttgctctt
    80161 cggctacttt aaatgcttgt tcctgctttt gcctgttatc atccatcgca ttataaaatg
    80221 ccaatggcaa caatgcctct gccactttat tcttcacagc accttggtat aactttctta
    80281 ttaaggcaat tttttgtttt ctgacatcta cacttaaacc attgttgtag tctcttgcca
    80341 aatattctgc caactcatat tctgccggcc cataatcatt ggcagctgaa agataaagca
    80401 tggccatagc ttgctcatca tttgctttaa tatactcttt tccatcagga ccaacgatcc
    80461 cctgacgcaa tatccttgcc aatacgtatt gtgctctctt attcccttta aatgcggaat
    80521 cagtcagcca atttaatgct tgttggtaat cattttcgtc tttaccgcgt ttcagataaa
    80581 gtattcctaa attatattca gcacccaagt gcttttgttc tgcggcattc tcataaaaaa
    80641 taatagccga agcatcatct tgcattaaac caatcccata ttgaaacaat tgaccaattt
    80701 caaattgagt ttgagcattt ccgagtattg ccctgaaata caaataattg accacagaca
    80761 tataattgac agtccgtttc caatcccctg tccaaatgtc catcaaatca aaactgccaa
    80821 cttcgctatc atcataattc gcgtcagcat aaggaactgg caagttgagc ttttgtacaa
    80881 caggactatt ggctctctct agagcttcta caaatgggtt aagtctgtaa gttggataag
    80941 cccattgatt tgattgaaac tccacttgtt tactgattaa agcatcatag taattgctta
    81001 taggaatact attaggctga gtcaactcaa attgtggttt gaaaatatcc tgacgagaga
    81061 tggtttcaag cttaggtgct tgattatagc tattatttct cagggcagac ggattattcc
    81121 atgggctaaa tataccattc atctggtaag ccgtctcttc caatttatcc gttctgtcat
    81181 tacttagcca taatgcagct tgggcaagaa caggaggctc tgcatttttc ttctcgtttt
    81241 ctgctgcttt aagagtccat tgtttagaca attcttcatc agccgctacg ccaatccctt
    81301 tttgatataa attggctaat tctttttggg cttgaacaga accgttctga gctgctttta
    81361 gtaccaataa aaaacctgtt tttggatcat aaactggact tttatcttgt aaataaatat
    81421 gggctaactc aagagtagct tctgtactct gttcctcggc cttattaagc cattgaatag
    81481 cctcatcgta ttgattttgc attaaagcca atttacccaa ctcaagcatg gctggagtat
    81541 attgttctga ggctgcttta tttaataatt caacgccctt ggcagtatcc ttggcaacca
    81601 tttgtccggc gacataaaat ctgcccaatt ctgttaaagc ttttggatct ccattttctg
    81661 ctgctttatt cagccaaatg atccctaatt ttttattacc cgcatgctta ctctctaaga
    81721 aatactctgc taaggtgtat tgagcaatag gattaccatt ttttgctgca tcaatataat
    81781 aacgtcttgc gatatctgta tttttcttga cgccaacacc atataaataa gctgccgcca
    81841 tatacatttg tgcgctcaca tctcccaccg cagctgcttg cttaaaccaa cgagcagctt
    81901 cttccggatt tttttcatgg agcaaactgt atttggccat gagcaagaca gcaggtaaat
    81961 agcctttttc cgctgatttg gtaaagtagc gcatcgcaac acgattgttt tttaactgcc
    82021 catatccata gagataaagt cgccctaaat agtattcggc aacagcatcc tgacctgatt
    82081 taatgattag gggctcagct gcttcattat aattgcctaa tctataagca ttgaacgcat
    82141 cattggcaaa agcctgttgg ccagctgacg cgactaaaac acacacccaa ggtactagtg
    82201 atttcatgaa gattcccctc ttttcatggc catttataca ccgggtataa tgccataatg
    82261 caccacttga ctaactgccc catttctcgt attgctgtct actatccaaa aattaccttt
    82321 attattcaaa ccaaatttga cattaacata ttcgcatact ttaatcacat cagcacaatc
    82381 cagtattttt ccataggact ttttgccatc acccaaggta cagatgaact tcacttcctt
    82441 ttcacctgtg gccaaaacag cccaggaatt ccctctgata gagtgattta gccaatagga
    82501 atataaactt tccttatcat gcatctgata taagctaacg gtttctggca atgtattata
    82561 aataaaatac attgactgca aggctccttc cttccctgga agaagagtca atacctttaa
    82621 attttgctga tagccaacag cagtacaacc gagaggaaat tttccgttat cttttttctc
    82681 cgccttatct tcggcggttg ctgttttatt agatttggcg gcatatgccg acatatttag
    82741 tgcaccagtt aatagaatta caatgattgc agttatcgag cttcggcttt ttaggttcat
    82801 tttttattct cactatcatt taaaggacgt acaatcacct tagtccccat aaaattgtta
    82861 cgctcactgg acaactctac aaaattttcg tttaaccact tggcatcctg gataaacata
    82921 cgcacacaac catggctagc ccttaccccc ggaatatcat cagaaccatg taaagcaaac
    82981 cctttatgaa aatacatgca ataaggcatt ttggcaccgc ctctgcctat aggaaaaaca
    83041 tctgaagtac aacgaacatt ttccttgctg aatacacgga aaattcctgt caaagtacga
    83101 cagactttat tggaatcaga gcatttatca cttccagaag aaatgggtcc ccaccatact
    83161 aaattgccct gagcatcata ggcgccccaa gccagcttct cttgatccac gataatttgt
    83221 ttttcatttt cactctgaat tttaagtggg aaaggagaga catcaaaaat attgatgttg
    83281 gctaaattct ttggtacagc gatttctctg cctgaccaaa ggtagttata agtacgattg
    83341 actctttgca caatgtctct ttggacagga tcagggaata atttttccca actttgtccc
    83401 ccggttacct taatacattc atattgagga taagcacata atgccgttcc ataaaaatgc
    83461 ccagcgttaa gtacagtcac cattgacatc atgaacgttg ttaatagtat ttttttcatg
    83521 gtgccgcccc cttatgcctg atttttataa ttttatgcaa aatttgtgct aaaaatgaaa
    83581 tgccactctt tttatctttt ttcggttatt tttaatttaa gtttagaccc atacaaaaaa
    83641 aaattgaaat tattgctata tttaaattag cattgactgg gttgaacact catgagtcct
    83701 ttaaaaagta aaattaaaac aaagtctttc aacaaattaa caaaaactca agacattacc
    83761 aacacgattg aaagattatt tgcaaactct caagacagca tcattgtttt taatcaacaa
    83821 ggcaaaatta ttgctattaa tgaaaaagcc gctgctttat ttaaaaaaag ccctgacaaa
    83881 ctaattgaca aaccgatttg gaaactattt aagttaaata gcttcgctat taagaggcaa
    83941 tttattcaag caaagaggtg ttttctttta gccaatgaag ggatagccca gcaattcact
    84001 tggcttgaaa gcaaagcaca aaaaccagta cttgcatacc atatcctgtt caataaaaca
    84061 gaaatagaag acacatcaat catttttacc aaacttaccg atattctaaa atcgaaaacc
    84121 atagaatgga ttttatggtc tttaactaaa ataagtaacc accaggaaat caatgaaatt
    84181 attgatcaaa ttctacaatt aaccagtgat gtttttgcag cggattatac ttctgtatct
    84241 ttcataaaca atcaacaaat tgcaaggaca atcagctact ataaatttgg aaagaaagaa
    84301 gagaacatta gtttttcttt aatcaataca ccctgcgcta aagtaatcca gaaaaaagct
    84361 atctgttatt ttaatgacgt tcaaaagcaa tttccaaacg ataaattatt gaaaaggatg
    84421 aacatcaata cttatctcgc aggcccaatt acaaatcccc aggatgaggt gattggcata
    84481 atgactatcc tgtgtaaacg caaagtcgaa tttgacacac tgaataatac cttgtttggt
    84541 ttatttttaa gccgaatcaa ttcggagatt gagagactga taaaccttag aaaattagag
    84601 tttctggcga gtatacccca gcaaaaccct aatccaatca taaggattct tctcaatggg
    84661 gatgtcgtat ttactaacga tgaagggaaa atcattttaa actactggat aaaattattt
    84721 aagggacttc cagtagagtt actaaaagaa gcacaaagaa ttcagaaatc caatcaaagc
    84781 acaagaattg aaatggaaat tgaggataaa atctacttat tcacacttgt ctggattgac
    84841 gaatttaaac agatcaatat ttacgggact gatataaccc agctcaagaa tacgcaaaac
    84901 gctatgttaa atctaacccg cagagatact ttaactcaaa tcg
//
LOCUS       DBNCWK010000002        68236 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.002, whole genome shotgun sequence.
ACCESSION   DBNCWK010000002 DBNCWK010000000
VERSION     DBNCWK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 68236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68236
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.002"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            57..746
                     /locus_tag="AC06IO_000071"
     CDS             57..746
                     /locus_tag="AC06IO_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIO9873295.1"
                     /translation="MDMNIKEKIGQRIMNERKAKGLTRKALAELTGELKTSRINNYER
                     GDRTPGPTEIKLLADALEVSASYLMCLTDNREGKMTKSLGMGALIPVLDYKQAADPVT
                     YIQKIKDDIDSKVEFVPVSKAVGEKIGKNAFALQVRDESMMPEFRVDDVIIVDPDTCP
                     KPGDFVIALVEGENEVIVRKYRQLSLSKKEQEFELVALNNDWADLRVDASTFKVLIVG
                     SGISLCRELRD"
     gene            complement(887..1744)
                     /locus_tag="AC06IO_000072"
     CDS             complement(887..1744)
                     /locus_tag="AC06IO_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873296.1"
                     /translation="MKKLTITLMSLSSINLYHPIAFAENSHHHTMTHVSLESSQSEAR
                     PTMQLSIQEVMDKENKKLVLIKLSDTKNNKPITLGNLIEAHTQKIHLLIIDDHLVDYS
                     HVHPIETKIPGVYQFEWQPTLKNASYRAWADLIPANTKNQEYVVADFPSPKIIKRIED
                     RMVRQPFFESTVDGYKFKLTFDKTPLHVGQPIMGKIDIVDANGKPVHTLEPIMGAYAH
                     IVGFNEDFKTVTHVHPMGKEPTNGSERGGPELQFHIEPDKAGFIKMFAQVQINGKTLF
                     VPFGILVTQ"
     gene            1950..2096
                     /locus_tag="AC06IO_000073"
     CDS             1950..2096
                     /locus_tag="AC06IO_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873297.1"
                     /translation="MKQRHWIILACCLALAACMNNGTSTKNTESLDSHVAQGHVGHGG
                     GGGR"
     gene            2225..4918
                     /locus_tag="AC06IO_000074"
     CDS             2225..4918
                     /locus_tag="AC06IO_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIO9873298.1"
                     /translation="MAEQWHEKTIQDIASQFKIDLTVGLSEAYATERLKEVGPNLLSQ
                     KKKTSALIIFFQQFSNIVIWVLLGAVIVSFLLGEKADAIAIFAIVILNAIIGFALEYR
                     ADRAMAALQEMAAPKATVLRDGHAKMIAASDIVPGDVILFESGDLIAADARLFKLSAL
                     KVNEAPLTGESMPVGKNLDLCTQETPLADRKNMVFMGTAIADGTGRALVVATGMQTEM
                     GRIAQLLGEASRDKTPLQKKLNQVGSRLLWVCFFIVMAIFGLGLLRNIPIFNLFMSSV
                     SLAVAAIPEGLPAVVTVALALGVQRMVRRAVLVRRLSAVETLGYLQVICTDKTGTLTV
                     GEMTARKFVTASDVYSIYGEGYNLTGKFSLQGQEINVSEDKLLQASLQAMVACNNAEF
                     RQQNGQMTIAGDPTEVALLVAAAKGGFWRSELQISNPRIKELPFSSERKRMTVVCQYD
                     NDELMVYVKGAPEIVLERCTHILTKTGIKKLTLNDKARMRQSCEFMASEALRILAFAQ
                     RPLDTSWLDKEVDEIESNFVFLGLVGLQDPPHASARESVSRCKKAGIKLVMITGDHPV
                     TARAIAQELGILSEGDQLLTGNELENMSEEEFNYCVKDIVVYARVTAEHKLKIVRAWK
                     KQNRVVAMTGDGVNDAPALKEASIGIAMGKTGASVTKEASDIIVMDNNFTSIVAGIEE
                     GRTIYDNIIKTLIYLLAGNSAELLVVFVALLIGWPLPLLPIQLLWINLVTDGLPAIGL
                     ATDRSEPGILNCPPRTIQKSMIDRAFLKRVSFIGCVTALVTLTVFAYEYLAYKDLTQA
                     QDAAFSILVTARLLIAFGARSDTKTIFQLGFFSNLHLFIIISISFSLQVLIHHIPVLR
                     DLFGIGQISFTQCISWILIGTLPLLVIEAQKWLRKAPGETF"
     gene            4905..6161
                     /locus_tag="AC06IO_000075"
     CDS             4905..6161
                     /locus_tag="AC06IO_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIO9873299.1"
                     /translation="MRHFSIRKIIWIVLLSFCFTESFIYAKPNPGLSQLIQEAKQNNP
                     QIRAVRDRLLAAIHVIPQAKALPDPKLNAGYINMSENIPMDVDPRREQMLGGQQEIPF
                     PGKLVVRGRIATLEAKRAEAEYQATCFAVIAELKRLYYDLYFVNKSIEIVQRNQELLH
                     EMEQSAEATYSVGKTPQQDIYRAQTEVSRLEMRLVILKQQRQSLQADINRLLNRSLER
                     VVSTPTTLPVTPMGHNLEYFYSLVKQRAPQLIMQQRNVQKGRQAIKLSKMEYFPDVEI
                     EGGRLHDTGMHTKGYQVLLKATVPLYFMQKQNNAVRESLARYNADMEDLQTTYRTLSF
                     QVKNAYLLAERSAKLIHLIQHTIIPQATLTFTSSQANYGVGKVDFLTMLNNLLTLQEN
                     ELEWHGELAEHEKAIAQIEESTGTYL"
     gene            6158..7291
                     /locus_tag="AC06IO_000076"
     CDS             6158..7291
                     /locus_tag="AC06IO_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9873300.1"
                     /translation="MNQKVMRRGVFVLSLLALLQLTQAQNATDTHSKMDMKQSTQKNE
                     IVIDPVRLQAIGITSEPVRRQNIEKTIRTVGRVEADERRVAHIHVRFDGWIEQLFINF
                     TGEKVQKGQALFTVYSPELVATQQEYLLALNAQKILSKNTTSSAARGAQGAFQAAHQR
                     LLLWGISEKEIEQLKRTGKITRTMTIDSPIQGTVLKKMALIGMRIEPGDELYTIADLS
                     RLWVLGDIYEYELPYIKLGQTADLTLTYLPNELFKAKLDFIYPTIDMKTRTAKVRFEV
                     DNTKEQLKPGMYVNLELKIPLGERLVVPKNAVLLTGERAVVFIYHGNGKIEWRDVTLG
                     VRAGDLLEIVKGVKEGDKIITSANFLIDSESQLKAAMGGMQHK"
     gene            7386..10529
                     /locus_tag="AC06IO_000077"
     CDS             7386..10529
                     /locus_tag="AC06IO_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIO9873301.1"
                     /translation="MVERIIEFCARNRFIVLLFVLGLSFMGYMALKNTRMDALPDISD
                     TQVIVYTTWMGRSPDLMEDQVTYPIVTALLSAPKVVAVRGFSDFGFSYVYIIFEDGTD
                     IYWARSRVLEYMSQLAGKLPEGVTPQLGPDATPVGWIYQYALVDENGKHNLAELRTFQ
                     DWYLRYWLRAVPGVSEVASVGGFVRQYQVNLDPVKVLAYNLSVPDIIEKIRMSNNDTG
                     GRVVEFSGVEYMIRGRGYIKSTEDIEKIAIGTNANGTPILLRDVATVQLGSDMRRGVV
                     DLNGQGEAVGGIVIMRFGEDVLQVIGRIKEKLKELATAIPEGIKMVPVYDRSNLIREA
                     ISTANWNLIEELVVVGLLIIVFLGHFRSALIPIITLPLAILISFIPIYFLHIGLNIMS
                     IGGIIVAVGDMVDAAIIMVDNAHKRLADWEAKGSPGDRTQVLIDSAKEVGPAIFSSLL
                     VIAISFMPVFTLEAQEGRMFSPLAYTKNLAIFMSALLAITLIPVLLPLLVRGRIIPEQ
                     KHPITRLMQAMYAPVLTLALSYRKVVVGVAIVVALATIPLYKLIGAEFMPPLYEGTIL
                     YMPVTLPGISVTQATALLQEMDKKLKAFPEVAHVFGKTGRAETSTDPSPFSMMEVIVE
                     LKPKEFWRKGMTYEGLVAEMDKALQFPGVSNAWTMPIKARNDMLTTGIRTAVGIKIFG
                     PDIKKIEAIGKEIEMVAKEVKGTSNVYAERVAGGYFLDFQINRDQLARYGLTIMDVNR
                     IIESAVGGENITTTIEGRERYPVNVRYLRELRDDPDKLNRILVKTPSGAEVPIAQLVT
                     LTFRSGPAMVRDENAMLAGYVFIDISGRDIGGYVEELKQAVKAKVKLPPGYTLAWSGQ
                     YEFMQRVYERLKIFVPLTLVIIFVLFYFTFRTITETLMVMLGVPFALFGGFLLLYVLG
                     YNMSIAVWVGMIALAGVAAETSAVMLAYLDSDYNEQKEKGLLKTLPDLIQMVHLCAIS
                     RIRPMVMAGLANIIGLLPVMWATGIGADVMKRLAAPMVGGVFSALLLTLIVIPVVYVM
                     WRWQVELKQQGSLTEAK"
     gene            10526..11887
                     /locus_tag="AC06IO_000078"
     CDS             10526..11887
                     /locus_tag="AC06IO_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIO9873302.1"
                     /translation="MKLTFLGATETVTGSKYLLTVDSKKILIDCGLFQGYKELRLRNW
                     APLPVNPRDIDAVIITHAHIDHTGYLPLLIKNGFQGKIYATTGTKALCSILLPDSGHL
                     QEEEARLANKYGFSKHRPALPLYTEQEARVALNYFETIDFDTPHSLFNVLSFEFHRAG
                     HIVGAAMVKIKTKKGSMVFTGDLGRPHDPVMKAPSVIQETDYLVMESTYGDRLHDATD
                     PLPQIGEVINRTIKRGGSVIIPAFAVGRAQSLLYFIYELKRKGEIPKEIPVFLDSPMA
                     INATKLLCTYKEDHHLNEEQCRGLCNVATYINTPEESKEIDRHKMPQIIIAASGMAQG
                     GRILHHLKAFAPDPKNTLLFTGFQAGGTRGARMVNGEREVKIHGGLVPIRAQVVVMSS
                     TSAHADYLEMLEWLKHFTHPPKKVFITHGELHSAQSLKEKIESQLGFPCTIPSYLQEE
                     DLS"
     gene            11845..13398
                     /locus_tag="AC06IO_000079"
     CDS             11845..13398
                     /locus_tag="AC06IO_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HIO9873303.1"
                     /translation="MYDSFLSARGGFVVIQKEHHRLRLKYLGIKTYHEAIIYMREDCH
                     VCHSEGFEVQTRIQVTLGQRSIIATLNVVTSELLRPGEASLSNFAWDFLNAKEGDDIQ
                     LSHPKPLESLSYVHTKIYGNELSFKQIKAIVDDVLSGRLSDLQISAFLAASAAERLSN
                     IEIVKLTKAMIDSGDRLSWSSPLIVDKHCVGGLPGNRTTLVVVPIVAAFGLTIPKTSS
                     RAITSPAGTADTMETLAPVSLSPEAMCQVVDKENGCIVWGGAVSLSPADDVLIRVERA
                     LELDSEGQLVASILSKKIATGATHAVIDIPVGPTAKVRNKSRALLLKQSLEEVGKQLG
                     LVVHTLITDGAQPIGNGIGPALEARDVLAVLQGLPHAPKDLREKSLTLAGAVLEFSST
                     VPPGSGQSIAKQLLDSGEAFKKFQAICEAQGGMRELTVARFTHPMVAEKAGKVSLIDN
                     RMLAKIAKLAGAPKSKSAGVDLHVHVGDQVEKGQPLFTIHSESSGELTYARDFIRDKQ
                     NTIVLGEKP"
     gene            13395..14300
                     /locus_tag="AC06IO_000080"
     CDS             13395..14300
                     /locus_tag="AC06IO_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIO9873304.1"
                     /translation="MKPILFSLFDASGISRRLKEALQVEVGDVTLHCFPDTEWYVKIN
                     SAVKNRSLIVVDSLNQPDGKILPLLFFAKTAKELGAKKIGLIAPYLPYLRQDKRFHQG
                     EGITSRYFAELLSTSFDWLMTIDPHLHRYHSLDEIYSIPTFVLHVTANIATWIKNHVN
                     KPVIIGPDMESQQWVADIAEKGHFPYVILEKIRRGDKEVSISVPTIPDLESSTPVLVD
                     DIISTARTMVETVTHLQKAGAQSVICIGVHALFAEEAYSLLSGMDGVQIITCNTIRHI
                     TNAIDVSEVIATTLEKYKLAKVLKK"
     gene            14338..14508
                     /locus_tag="AC06IO_000081"
     CDS             14338..14508
                     /locus_tag="AC06IO_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873305.1"
                     /translation="MSLFKIIRVIGMVIGFSAIFFLSSCNHAVKQSSQETDGGYGGHS
                     AGGHGSHGGVGH"
     gene            14529..16745
                     /locus_tag="AC06IO_000082"
     CDS             14529..16745
                     /locus_tag="AC06IO_000082"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-transporting P-type ATPase"
                     /protein_id="HIO9873306.1"
                     /translation="MKQNPHQGHPNSGKEHASCHQEHKTPKPPVPSSKREGSIVYTCP
                     MHPEIRQSAPGNCPLCGMALEPETVMVSEAASPEYLDMRRRFWIALILTVPIVILEMG
                     GHGLQHFISGDVSSWIQLLLATPVVLWGGWPFFKRGWQSLKTHQLNMFTLIAMGIGVA
                     WAYSVVAVLWPGVFPQAFRNQEGVVAVYFEAAAVITTLVLLGQVLELKAREQTGSAIR
                     ALLKLAPERAHLIKEDGREEEVSLDKVQVGDKLRVRPGEKIPVDGEVLEGRSFVDESM
                     VTGEPIPVTKETGAKVIGATINQTGSFVMSALHVGSDTMLARIVQMVSEAQRSRAPIQ
                     RLADTVSGWFVPAVILVALLSFILWALLGPQPSLSYGLIAAVSVLIIACPCALGLATP
                     MSIMVGVGKGAQNGVLIKNAESLERMEKVNTLVVDKTGTLTEGRPKLTRIVTDGEFDE
                     EVILGFAATLEHQSEHPLGHAIVTAAKEKELSFGSVEDFEAPTGKGVLGKVNGHRIAI
                     GNLKLMQDHGSDDASLLEKADELRAQGASVMFIAVDGKTAALLVVEDPIKSTTPETIH
                     ELQQSGIDIVMLTGDSKKTAEAVAGQLGIKKVVAEIMPEDKSRIVGELKEKGLIVAMA
                     GDGVNDAPALAKADIGIAMGTGTDVAIESAGITLLHGNLRGIAKARRLSESTMSNIRQ
                     NLFFAFIYNMLGVPLAAGILYPLTGLLLSPIIAALAMALSSVSVIVNALRLRRLIL"
     gene            16828..17709
                     /locus_tag="AC06IO_000083"
     CDS             16828..17709
                     /locus_tag="AC06IO_000083"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIO9873307.1"
                     /translation="MVIPVFMILLGIGFLINGAYLINKLLQQTNHYRRQYLVYLAFIG
                     VFILSYSASIVYGLLVDPHCLNYFYATVFLLGATYIDSSCHLMTKTMQKMELMDDFKN
                     RYETLRHHATYDSLTGCNNREYLFEMLNTRFQQIQLHGGEVVVLFIDLDRFKAVNDRF
                     GHDTGDTVLQTFGQFLRQRLRKDDVVARYGGDEFIVLMENTSLSEAKKIAENLVTAAT
                     QSLNGELLDTLDLGCSIGITQMSRHSVSVNAAIKEADLACYAAKQRKINGAVCVYSPM
                     LATTSNHMVSCIKQMTE"
     gene            17878..18252
                     /locus_tag="AC06IO_000084"
     CDS             17878..18252
                     /locus_tag="AC06IO_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HIO9873308.1"
                     /translation="MTHSLMKSALLFIVPAVLMGCSYNAKLTPEGEKVILLKAEKRLK
                     TCTFLGEVRAYDRNGMSQSYQSHEHLIQDELNILRNKAALLGANTLFVNEHQQTYEGN
                     PKNDLVADHWMVGRAYQCKTKQ"
     gene            18274..19596
                     /locus_tag="AC06IO_000085"
     CDS             18274..19596
                     /locus_tag="AC06IO_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIO9873309.1"
                     /translation="MENNRVWLFSCWVVLLWLYSSLANALTLQEITQIALANNKDLKA
                     ARYNVAIAKARLIQAGLWPNPSLNLSNNDDRLFNDEGEYSRSAGFSQAFPISGRIAKQ
                     KTVARLDVLKAMAEIGEAERQLSAKVANAFYAAVITERRLQQLNYLLKINKELVNVIH
                     NRYNAAEISKLDENAARIEYLRIAQEKHLQHSLRISQNATLNQLMGRQADTPLSLKGD
                     LATGRQLPKLALLKTWALNHRPDRQAIVLSMSRANADHRLAKAERFADWTVGLGVQQD
                     KIVVEEGSPQPADRTLGVTLSVPLPLLNRNQGRILEASHTATQAMMALSALNLSIETE
                     IASNYGQLKALKMSLRETETVSLKLGVENIKLAQDSYENGQISLLNVLQVQKQQNDLQ
                     MTYLATREKYLQVYVALCTALGPGNTQGFCPYLAYQRNGDSQHVTTTK"
     gene            19577..20542
                     /locus_tag="AC06IO_000086"
     CDS             19577..20542
                     /locus_tag="AC06IO_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9873310.1"
                     /translation="MLRQRNRRLAFISIFLSLFLSMQGCFAHGEEIEVSEGGAKGPVH
                     LTPQQTKMLGIKVAEATNRPMEQMLGLNGQIQLLPDAQADVSIRISGSVTAIDVNLGD
                     IVKAGQRLATVQSRLVGNPPPSVAVTAPIAGLIDARNINLGQAVEPNTVLFHISNRDK
                     LFVIAQVYEEDLGKIKVGQAVNIHALSYPRQIFKGQMTLIEPNLDSLTRTVNVRITLD
                     NKEGLLKPGMFVRANVILAYNEAALAVPSAALLQADNETFVFVQNDDTYDRVVVKVGA
                     VDDEHSEITDGLVPGDLVVTQGNRELYTLWLSGGSKPKAGQEEPH"
     gene            20543..23752
                     /locus_tag="AC06IO_000087"
     CDS             20543..23752
                     /locus_tag="AC06IO_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIO9873311.1"
                     /translation="MFTYLIAWSLRNRPLILALTIILCLLGGYTLQQMPVDVFPEFAP
                     PQVVVQTQVPGMATQDVETLVTYLLESAINGTPGVASVRSKTSVGLSTITVVFDDKTD
                     IYRNRQLVNERLQQAVNRLPPGANAPVMLPVTSAVGWLVKYALISRTASPEALRTISD
                     WTIRPRILALGGVAAVVSVGGEVKQYQVLLDSQRMVAYGITIEEVRQALTSANQNVPG
                     AFVHQAGTEFVVSTMGRIKTLDDIKKTLIVVRNGVPITINNVATVAFGGEIKRGDASY
                     NAKNAVIGTISKIYGADTLTTTYKVEQALEEIKRSLPAGVELVTEVFRQANFIESAIH
                     NLTRALIEGAIIVIAVLFLFLMNWRASFITFLSMPVSFIVGLLVLHYFGFGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRLNRLAKKSLPAIEIVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGISERIFSPLAIAYIASVMGSLVVSITTVPALCYLLLVGKTEKKQDT
                     QVVMHALPQEARYHLTEEEIAQHAQAETRFVMWLKKHFLTGLNWSLAHCKTVVTMAIG
                     AFLFALALLPFFGTSFLPEFHEGNFIIAMSTLPGTSLDETMRLGQQVSKTLLKYPQVV
                     SIAQRAGRSELDEDAMPPNASEFDVRLDFDKDKTMSPDELLRRIRADLANIPGAVFNV
                     GQFIAHRMDEVLSGVRAQVAVKLFGDNLATLNELGQSVEAILKTVPGVVDVNKEQQIK
                     VAQLVITIDREKAARYGVNVGQISEDVQVLLNGVTVSSVLEGQRTFDLYVRMGEEGRD
                     SMKAIQNMLIDAHGLANQETKIPLRAVADIQLEPQPFAINRENVQRVLVIAFNVQGRD
                     LGSVIQEVQQRIKEKVSLPSGYFIQYGGQFESQQQASRVIILFGALAIFIMLILLHKA
                     FGTFREALLVMFNLPLALIGGVVSLFLASGDMSVAAMIGFITLFGIAARNGIILVSHY
                     NQLRTQGQPREQVVIQGTLDRLVPVLMTAATAALGLIPLLWGSPSGKELERPLAQVLL
                     GGLFTSTFLNMFVVPTVYNTMETWREKRQYAKANSQGES"
     gene            23752..24204
                     /locus_tag="AC06IO_000088"
     CDS             23752..24204
                     /locus_tag="AC06IO_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIO9873312.1"
                     /translation="MNQITKSFNKKALGLFNLLVVLLFLLSPAYAAANHEEEAQSIPT
                     TIPAIWEAINQHTAAINKALADNQLDTIHQHAFAIRDLVKALPDLSKNLSEEQLKNLK
                     QNVGYTEQLAKRLDKTGDANDKEGTQANWQKLQKVLAQIRANYSLSGS"
     gene            complement(25092..26009)
                     /locus_tag="AC06IO_000089"
     CDS             complement(25092..26009)
                     /locus_tag="AC06IO_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIO9873313.1"
                     /translation="MRLRLLAFPLAGIIVGVSIYSGIINTIKEYVDNYVVQHKNPADW
                     NNRGIDYMIGKKVPQNYSEAVKWFQKAAEQGDTMAQRNLGLTYTTGTGVAQNHSKAMK
                     WFRKAAEKNDPVAEFNLGVLYIEGIGISHNDGEAVKWIHKAAEQGFPDAERTLGILYL
                     TGKGVKQNDGEAIIWFRKAGEHGDLASQRTLSAMYVEGNHTQQNDFEAMRWFYLAAKQ
                     GDPIAQYNIAIGFLVGKGMKQNNIEAMKWFHLAANQGLPQAQYALAAIYHDGQGVPQN
                     HDEALKWLQKAAEQGYPAAQQHLQNLRGE"
     gene            26295..28085
                     /locus_tag="AC06IO_000090"
     CDS             26295..28085
                     /locus_tag="AC06IO_000090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIO9873314.1"
                     /translation="MNGLEKQCCPKEVLLTQREELSVGTDSCCVPTDASTSDNFFPFF
                     NQLIQANLAKWTMGISPAAIGSSYSTWLWQLIQSPGNLWELAFYPIFHANDCINNIVC
                     VDRAAGGKDVRFKKDSWQPMPWRLFAEGFLQVEDWWRRATTNVPGLPRPVERTVSFWA
                     RQWLDALSPSNFVWSNPDLFNEAIRTGGLNLIQGSQIALDDWLEKLTGTPPTGSEHFI
                     PGQQVAITPGRVVFQNHLIELIQYESQTKTVYKEPILILPAWIMKYYILDLSPHNSLV
                     KWLVKQGHTVFIISWRNPDKEDRDLGMDDYYRLGAMAAIDAVSTILPEAKINLMGYCL
                     GGTLALITASVMGRDKDERLNSLTLLAAQGDFTEAGELMLFVTESQVDFLKSMMREQG
                     YLDTKQMAGSFQMLRAYDLIWSKMIQDYMHGMRRGMIDLTAWNADATRMPYKMHSEYL
                     EKLFLKNDFAEGRYTVEGKPVAAENIQLPVFAVSTEKDHVAPWQSVYKIHLMTDSDVT
                     FVLTGGGHNAGIVSEPGHPGRSYRIHEHKEGEAYLNPANWLTVAEKREGSWWRGWHEW
                     LVLQSTKKRIPPPVIDTSLPAAPGTYVHQK"
     gene            28098..29504
                     /locus_tag="AC06IO_000091"
     CDS             28098..29504
                     /locus_tag="AC06IO_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HIO9873315.1"
                     /translation="MEQEFLENVTFNELEVGRQASLSKTLTQDDINLFAAMSGDVNPA
                     HVDSVFAKSTIFHGIVGHGMWSGSLISALLGTVLPGPGTIYLEQDIQFKKPVRLGDTI
                     TITLMVKDKHTNKPIVIFGCKGINQRGETVIEGLATVLAPTEKMRVARAHLPPIEIYN
                     HDRFEAIIKTCRCMAAVRTAIVHPVTSNVMEAVNDAMKAGLITPILVGPLAKMRAAAR
                     DAHIDLSQWETMDTEHSDAAANKAADLAAAGEIDAIMKGSLSTHELMAAIVLSTSGLR
                     TQHRVSHAYVMDVPSYHKPLIVTDAAINIAPSASDKADICQNAIHLWRVLYGENKKPK
                     VALLAAIETVNPKMQATVDASILCKMADRGQITHGILDGPLAFDNAINKEAAKEKGIV
                     SLVAGDADILLVPEIESGNILAKQLTFLGHADAAGIVLGARVPIILVSRADSLRTRLF
                     SCALAVKMAAARKEGRIK"
     gene            29501..29884
                     /locus_tag="AC06IO_000092"
     CDS             29501..29884
                     /locus_tag="AC06IO_000092"
                     /inference="COORDINATES: protein motif:HMM:NF013067.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873316.1"
                     /translation="MTNIKASCLLVINVGSSSIKFQLFSRQPNPKILAYGKVVDIGCK
                     PSFTVTDDLKILEESENEKARFALKYFCLKMAQFMGMMAMALGEVDAIVFTGGTGENS
                     AFVRDGILRQLEFLKLVLLLPMKNG"
     gene            29937..30692
                     /gene="fabI"
                     /locus_tag="AC06IO_000093"
     CDS             29937..30692
                     /gene="fabI"
                     /locus_tag="AC06IO_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIO9873317.1"
                     /translation="MKNKKGLIIGIANEHSIAWGCAKVLYEANCELAITYQNEKAKSY
                     VQSLAEQVSASIFMPLDVTDKAQFDALFQRIKESWSHLDFVIHAIAFAPKADLQGRVV
                     DCSKEGFMVAMDVSCHSLIRLAKAAEPLMVNGGSIMTMSYYGAEKVVKNYNLMGPVKA
                     ALETSVRYLAMELGSKKIRVNAISPGPISTRAASGLADFDKLMEKAANEAPLHQLVTI
                     EAIGEMAAFLVSDKAVSITGQILYVDAGYNIKG"
     gene            30966..32210
                     /locus_tag="AC06IO_000094"
     CDS             30966..32210
                     /locus_tag="AC06IO_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIO9873318.1"
                     /translation="MGLFLRLVSLVLLSLSFSTKANTTFTFKQALAMAYRNNPELQAE
                     IDKAQAMRGAFIQSGLYPNPQLNLTAENFGGSGVYSSYESAETTASVTQPIPLGHRLQ
                     YLQKATYADYLTSLASIKVQKTVLYMAVGNAYVDALYAEQWHKVTKKLTKLNQDIVVA
                     IERRVKAGAGAELDLRLAQVRLGEARIQESKASRDALLQRARLARLLGYGLRIDKPLV
                     DKGLPGLTLDWSELIKKLPQSPQLVQMQLQLQARRATITAVKKSVWPDLNIQLGGRHF
                     SDDGSNAAVMSAFAEVPVYNRNQGKIMTAEAQYTQAAHEFQSTRLEVRQNVYAVFLQA
                     QQSQYEANLVTDSLLPSARKSIQLAQEGYQMGRYTYVELYTALSTLYEEERHYQQAHA
                     DYHKSLIQMTGLLGLEPIKESQ"
     gene            32207..33457
                     /locus_tag="AC06IO_000095"
     CDS             32207..33457
                     /locus_tag="AC06IO_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9873319.1"
                     /translation="MKFQRPFIPYFLSLILLLSFSSFSLRAENNHSEESEHQKTMEKG
                     PQGGRLFKEGNTALELLIFERGMPPRFRAYLYQNGKMISPDKAHLTVELTRFNDKKEV
                     ITFIPVENFLQSNQVIREPHSFDVTIQLTLQEKRYNWHYASYEGRVKIVAEVLKAADI
                     QMATAQSQTIKTQLKVVGKIAPNRDTLAPIYPRYSGIIQSMTKNLGDEVMKGEVLVTI
                     ESNESLQNYTITAPITGTIVQKYATNGELAQNTKPIYEVANLATVWADFTLYRKEAPL
                     VKQGMEVTVTGDEGKPKSISTISYISPLGVEDSQTTLARAVLSNDRRLWLPGMYVNGA
                     ITIKEKTVPVAVLLSAIQRMDGKEVVFVQQGDYFEATPVILGEKDSQWAEVVSGLDVE
                     QRYVSKNSFFMKAELGKEGASHEH"
     gene            33467..36625
                     /locus_tag="AC06IO_000096"
     CDS             33467..36625
                     /locus_tag="AC06IO_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIO9873320.1"
                     /translation="MLEKIIRFSLKHRWFVLLFTLVIAILGVYNFQRLPIDAVPDITN
                     VQVQINTQASGYSPFEVEQRITFPIELAMSGLPSLDYTRSLSRYGLSQVTVVFKDGTN
                     IYFARQLINERLQEVKDKLPPGVETTLGPISTGLGEIFMYTVTNKPNVPISQHYNPTE
                     LRTIQDWIIKPQLRNVEGVAEVNTIGGYEKQFHITPDPSKLVRYRLSLNDVVEALERN
                     NANVGAGYIETNGEQNLIRVPGQVQNMADIENIVIASFEGTPVRIRDVAEVALGKELR
                     TGAATENSKEVVLGTVFMLMGENSRTVSERVAAKMKDINKTLPEGVEAITVYNRTTLV
                     NATINTVKNNLLEGALLVCVILFLFLGNIRAALITAMVIPLSMLLTITGMVENQISAN
                     LMSLGALDFGLIVDGAVIIVENCIKHLAEQQHALHRVLNLEERLKVISYATTEVIRPS
                     IFGVFIITVVYLPILTLTGVEGKMFLPMAQTVIIALLASMLFALTFVPAAVAIFLRGH
                     LQEKENWLVHYLSLGYAKVLRRCFHARRVVISAAVALVVVSLGIAFHLGGEFIPSLDE
                     GDIAMHAMRIPGTSLTQAITMQDLVEKRIRQFSEVKNVFAKLGTAEVATDPMPPNVAD
                     TFIILKSRKKWTNPKKTKPGLVQEIESAVQQIPGNNYEFTQPIQMRFNELISGVRSDV
                     AVKVFGDDMDTLLKTAEAISAQLKQVPGAADVKVEQVSGLPLLTVEINRDVLARYGLQ
                     IGTVQEAVVIATGGKKGGELFEGDKRFDIVVRLPESLRSDPNVLRQIFIPLPLSKDGE
                     QHFIPLSEVASLIRSESPNQISRENGKRRVVVTANVRNRDLSSFVSEAKKRIDGQVKL
                     PSGYWITWGGQFEQLQSAYQRLQIVVPITLLGIFLLLFISFGKVRDALLVFTGIPLAL
                     TGGVFALWLRGIPLSISAGVGFIALSGVAVLNGLVMITFINKLREQKKVYLKDAVLQG
                     SLARLRPVLMTALVASLGFVPMALATGTGSEVQRPLATVVIGGIISSTFLTLLVLPGL
                     YYVFHGRRKKGQSKSEPQEQIM"
     gene            complement(36665..36865)
                     /locus_tag="AC06IO_000097"
     CDS             complement(36665..36865)
                     /locus_tag="AC06IO_000097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873321.1"
                     /translation="MNFLFSHMPLTIAVLAFLLGMLMMIATKNQHSKLKRAIATSGFL
                     LPFFGLIALIAILPLTYLPELR"
     gene            37007..37303
                     /locus_tag="AC06IO_000098"
     CDS             37007..37303
                     /locus_tag="AC06IO_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIO9873322.1"
                     /translation="MLTEDQIITLSDILHLMGEANRLKLLLRCLEGPKSVSELAEQLQ
                     LSVPLTSHHLSLLRSARLLIATREGKHIYYSIYDRHVRCILEDMLKHFTEEMEN"
     gene            37305..37577
                     /locus_tag="AC06IO_000099"
     CDS             37305..37577
                     /locus_tag="AC06IO_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIO9873323.1"
                     /translation="MGFLKRIFGNGLSDHHGGYRNSHHGQRNDYREDSANLSRGKMNC
                     PRCQTSLTPGMRFCSECGSSLESALCICGAVIAIGAKFCGQCGKSL"
     gene            37574..39727
                     /locus_tag="AC06IO_000100"
     CDS             37574..39727
                     /locus_tag="AC06IO_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIO9873324.1"
                     /translation="MTTPLRLTTFFVAGLDCPAEEQLIRRQLQGIPEIEHLDFNFIAE
                     EVTIHHRLSSIDLLQQRIAALGMSVRSKSGIHPAKEGGRSLSDSSWLLIILAGVFALF
                     SELAAYFLGTEQSTWSIIPALLAMVLSGSPTFKKGWLALRTKAMNINSLMLIAISGAV
                     LIGEWPEAAMVTVLFALAERIERYSLDKARLAIRSLMQIAPEVARVKMDNGQWQTMPV
                     EEVPLEAVFRVRPGERIPLDGVVISGQSTVNQAPITGESMPVVKQVGDEVFAGSLNEH
                     GAFEVQVTKASGDTLLAKIGKAIEQAQAERAPTQRFVDQFAKYYTPIMVLIAVLIALF
                     PPLALGYPFYDWLYKALTLLVIACPCALVISTPVTVVSGLAAAAKHGLLIKGGSYLET
                     GYRLKLIALDKTGTLTEGKPIVTDFIVYDKSQTKEQLLLLAASLDSHSEHPVAHALVT
                     YWQQEQSNSSLLDVEEFFALPGRGVKGLVQQELYYIGNHQLAEDNQVCNSAIEQALKR
                     LEEEGKTTVILSNSSTVLAVFAVADTLRTTSQWAIARLHQQGIKTAMLTGDNAVTAHA
                     IAKQVGIDEVKANVLPTDKLQAIDFLIEQYKAVGMVGDGINDAPALAKATVSFAMGKG
                     TDTALETADVALMNDNLAMLPLYIDLSRNTARILRQNISLSIAIKGIFFFLALAGIAT
                     LWMAVFADMGASLIVVVNGLRLLNSHQGHSEIQEI"
     gene            39858..40022
                     /locus_tag="AC06IO_000101"
     CDS             39858..40022
                     /locus_tag="AC06IO_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873325.1"
                     /translation="MNTRGLKIFFLIGMFCLGYGLTACTKTTTSKEVHPESGYEGGFG
                     GDGEHRHPNQ"
     gene            40518..40982
                     /locus_tag="AC06IO_000102"
     CDS             40518..40982
                     /locus_tag="AC06IO_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIO9873326.1"
                     /translation="MAIRYLNFNSRINKLLSKVILLFLISISHTMASEQPASKVTIPA
                     TVPELLQEMDEYSLAIYKDLEMNRLSEVRQHAFVILDLINALPGLSQDLSPKQLKNIQ
                     SHIDAISHLIPDLIKSSNANDKKTTQRHLDKLQKLLNQLHTYYLKQKTEHFN"
     gene            41123..41422
                     /locus_tag="AC06IO_000103"
     CDS             41123..41422
                     /locus_tag="AC06IO_000103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIO9873327.1"
                     /translation="MNNSFRFLVVEASFAARIVIRSQLMQLGQLVDLATTAEEMFDKI
                     AVKTYDTLLIDNHLYNSLGDYDLIAFIKNNNPIVLLLLLLSLPNNRLLGIKSIPH"
     gene            complement(41586..41780)
                     /locus_tag="AC06IO_000104"
     CDS             complement(41586..41780)
                     /locus_tag="AC06IO_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873328.1"
                     /translation="MSEHYTAKIYCGTEEIARQSGENLEQLYVWILAYAEKTYGEIHG
                     EITDKISHQVVRRFKKQSIE"
     gene            41954..42124
                     /locus_tag="AC06IO_000105"
     CDS             41954..42124
                     /locus_tag="AC06IO_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIO9873329.1"
                     /translation="MADKVRGKVKWFNKAKGFGFIESNGKDYFVHFSAIQGNGFKNLP
                     DGARVLFKVGQG"
     gene            42217..42957
                     /gene="phbB"
                     /locus_tag="AC06IO_000106"
     CDS             42217..42957
                     /gene="phbB"
                     /locus_tag="AC06IO_000106"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIO9873330.1"
                     /translation="MERMTAVVTGGTGGIGTAICQHLAADYQVIACYFKNGQHEEAKR
                     WQATQKEAGFDVDILYADTTHFADCEKFTELVMERYGRIDVLVNNAGITCDTTLKKMT
                     LEQWQQVVDANLTSVFNMTRNVLPAMLDKGYGRIISISSINGRKGQFGQCNYAATKAG
                     LFGFSKSLALEVANKGITVNTISPGYIETPMLSAMREDVLKSIIANIPIGRLGKPQEI
                     AKAVAFLASSESGFITGANLDINGGQYM"
     gene            complement(43394..43636)
                     /locus_tag="AC06IO_000107"
     CDS             complement(43394..43636)
                     /locus_tag="AC06IO_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873331.1"
                     /translation="MECFIEVAEPVIDVKFQLKKDTQKYLIDYILSYSELDCKQLAQI
                     LKASPLVLSQVLAGKEFLGSAKARNLFHYFTMLIGH"
     gene            complement(43798..45279)
                     /locus_tag="AC06IO_000108"
     CDS             complement(43798..45279)
                     /locus_tag="AC06IO_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIO9873332.1"
                     /translation="MLSPEKMPKGIMPLYLIQAFSTFSYAILYSSLSLFLTKQLGLSN
                     ALSNSIVGLFLAFNYVLHLLGGVVGGRYLSNRALFLITTTIQTIGILLLALSVKSLLY
                     LGLSLFLVGCGLNTTAYNSILTQRFAPDDNRRDKAFFLSYSYMNVGFCAGYIISGFFD
                     YSNQYQTLLYASIIPNVITLLLMWGYWSNLNDRDTPLIKVTNKASLNLKKSFGWGIIL
                     ALIPFTLLCFHKAQFSNGVVVGLSVIMLFVILYLGYQQKSTLDKQKIMTFLILTVTSI
                     LFWMIYFTGPMGVTLFIKNNVDKHLFHYEMATQWILNINAIVIIIGAPLLSIVLTRLQ
                     AKGYNVSVTKQFIWAFLILAGSFFLLSSSILSANKHGYVSVYWVMLHFTTQAIAELLI
                     GPVGYAMIGRISPPQLQGVLMGTWMMVSGVSASLSHYFSNAMTKEESTNPLLSNGDYQ
                     HVFNQLGIWALLGAVFLYLIAKRIKPVIEQKTDSGLSETITAA"
     gene            45409..46713
                     /locus_tag="AC06IO_000109"
     CDS             45409..46713
                     /locus_tag="AC06IO_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873333.1"
                     /translation="MNKISRVSWDEIKYQVKQVNESIYHVIEEIKPGNTIPFFIARFK
                     FGEHFGIKNHAYLPTSSGQMEKIDSKHTDNELFNHLGYGKNSLPLGLILDKFCEWHYF
                     GEDERIFPDCVQGPGAIFNMQVIFDEDQTVDNNVLSVSSGALSSFLLPNIGCKRKHAR
                     IQKYFNVAAPAPKSPYEHYLVFKEILKDKNTIDNWYSEILYFSEQFINEVKHNDKWLK
                     LKLYFSESLRKKLTQNTYDASCNDLFLSAKSINRFRPTPFIMDTAKYIFNICMGSGIG
                     VKPAIDEQYFPVKTIQKIYNECYDLEYTATVMVPSALNGQSDSVYYPLQCPFAKINTF
                     KTNQSNSAYSELEMLKNVLLAYQSEFTADNSSAFGSLLYHVSKQTEFSFYHYKSSSNK
                     NINSSEELEGTDNRFAFSYCSDKTQFASDAKLFRGCVRLSKQ"
     gene            46724..47281
                     /locus_tag="AC06IO_000110"
     CDS             46724..47281
                     /locus_tag="AC06IO_000110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9873334.1"
                     /translation="MRILETERLFLRTFQENDVESLIAISQDQKVMQFFPAIPTREET
                     IAFIDRIISHQEEKNFSLYAVEIKNTGEMIGFVGLFTATFQAHFTPAIEIGWRLSSKH
                     WNQGYATEAAKAVLDYAFRKLDLDEVVSFTSELNKPSIRVMQKIGLYTNSEDNFDNPK
                     VTSGSPLMGHVLYRLKRSEYLSTQG"
     gene            complement(47267..48043)
                     /locus_tag="AC06IO_000111"
     CDS             complement(47267..48043)
                     /locus_tag="AC06IO_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIO9873335.1"
                     /translation="MHKLIVVGSGIKSISHLTEETKRVIQSADKVLYLINEDNLKQWI
                     QRETKNSESLDSIYFSSEKRIEAYQALTTHIIEEYKKVSILCVVFYGHPTVFADSALN
                     AVRQIKQDNGEAIILPAVSSQDCLFSDLEIDPGDQGCFSIEATELVLFERCIDIHAHL
                     ILCQVANFGRTDGKKTSSLSILKDYLSGYYPADYSICLYEAPSLPTYPPRIEWIQLRN
                     LELSAISSITTVYIPPIEKKAISKKYLKLLDLKIDDLALS"
     gene            48558..48851
                     /locus_tag="AC06IO_000112"
     CDS             48558..48851
                     /locus_tag="AC06IO_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9873336.1"
                     /translation="MKTMNILQASQFLGVHKESLRRMAANGQLPGVKIGRRWIFIEQD
                     LVMHIRNKYSTCDASQGAYVRSNKEWHSTKEMEFGGLISGTKEKEYAKVLGLK"
     gene            48764..49738
                     /locus_tag="AC06IO_000113"
     CDS             48764..49738
                     /locus_tag="AC06IO_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIO9873337.1"
                     /translation="MALYKRDGVWWLDIRHQGKRVRKSTGTEIREDALRLHDQLKHEL
                     WQTDKIKSLPNRTWMDAVLRWIDESSHKRSLETDKYHLAWLDSFLRAKKLSDIDRDLI
                     EFIAKKKEAEHVSLTTVNRVLELVRAILNRAHKEWGWLDKTPIIRMRKIENKRIRWLT
                     KEEANLLLKELPPHLEAMASFTLATGLRESNVTQLKWSEINLDKKHALIHADESKSKR
                     PIPVPLNKQAISILKAQQGRNSTYVFTYNGNPVTRCNNHAWQKALIRAGINDFRWHDL
                     RHTWASWHVQNGTPLHELQQLGGWSNYEMVLRYAHLSSAHLRAAAERV"
     gene            complement(49804..49880)
                     /locus_tag="AC06IO_000114"
     tRNA            complement(49804..49880)
                     /locus_tag="AC06IO_000114"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49844..49846),aa:Arg,seq:tcg)
     gene            complement(49941..50016)
                     /locus_tag="AC06IO_000115"
     tRNA            complement(49941..50016)
                     /locus_tag="AC06IO_000115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49981..49983),aa:Lys,seq:ttt)
     gene            complement(50072..50147)
                     /locus_tag="AC06IO_000116"
     tRNA            complement(50072..50147)
                     /locus_tag="AC06IO_000116"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50112..50114),aa:Lys,seq:ttt)
     gene            complement(50234..51112)
                     /locus_tag="AC06IO_000117"
     CDS             complement(50234..51112)
                     /locus_tag="AC06IO_000117"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase family protein"
                     /protein_id="HIO9873338.1"
                     /translation="MKWQIPAKTFLLGEYAAIAGEPAILLTTTPCFALTLTENAKLEG
                     IHPESPAGLWWQKNQCHPFGLSWQDPYHGQGGLGASSAQFLACYLATCSLQEITPRLN
                     HMLEAYYQSSWSGLGLRPSGYDVIAQSQQGCVYIDKQKNQVNSFSWPFKDLSFLIIHT
                     GNKLATHHHLQEVTLPSQIDYLASLVIKAKEAFDLAHSHHLIQCINLYAQKLTELNLV
                     SQHSLPLIQSIKSYPETLAIKGCGALGADTLLILTDKRDLQSLKDKVQDQNWLILATE
                     DNLTVRNNNSLFEKSI"
     gene            complement(51109..52407)
                     /locus_tag="AC06IO_000118"
     CDS             complement(51109..52407)
                     /locus_tag="AC06IO_000118"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIO9873339.1"
                     /translation="MSIAANASELFRGFSKLSREERFQRLCALGALTHEDITFLKQGG
                     IKDLNLADKLIENVIGYFQLPLGVATNFNIDGRDYVIPLAVEETSIIAALSKSAKWIR
                     QHGEINTWVHGECILGQIQLAKVKDFQRFSDLFNKNRQYFIEIANKDVAANMVKRGGG
                     VTDLQVRHLKREDGLDMAVIHLTMNSCDAMGANIINQVLEYLKQPIEQITGEEVTMCI
                     LSNLNDQKLTTAQVIIRNIDPILGQKLQEASLFAEIDPYRAATHNKGVMNGIDPVLIA
                     TGNDWRAVEAGIHAYAARSGQYKAITRWRYQNEILTGELTAPIIVGTVGGVTSLHPTA
                     KMCLRMMDITSANQLSQVIAAVGLVQNLGALKALCTDGIIQGHMKLHIDNLLLVAGAN
                     ENEMPVLKEKLQEWLNLNKRVSLNNAYDLLAEIRQAPVAV"
     gene            complement(52398..53426)
                     /gene="fni"
                     /locus_tag="AC06IO_000119"
     CDS             complement(52398..53426)
                     /gene="fni"
                     /locus_tag="AC06IO_000119"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIO9873340.1"
                     /translation="MQNEYSQFEQRKRDHIELALMPANQSSELNPFDHFSLVHEALPD
                     LDFKDISIQSIRFKKPVEKPFIISSMTAGHSNAIEINYRLMEACSKTKWAMGVGSQRR
                     ELTDKQAAFEWEPLRRDFPMVSLFSNLGIAQLIDTPISAIQRLIDTLHAEALIIHCNP
                     LQECIQPEGTTNFHGCWAALEALVKKINSPVIVKETGCGFSKNTLLRLNNIGVAAVDV
                     SGVGGTHWGRIEGHRADKDPIRHRTADTFRNWGIDTLQSTRNAISLNPSFEIWGSGGV
                     RNGLDAAKLFALGATTVGFAKPMLEAALDSTDQVLTQMNTIEYELKTAMFCTGSLVLD
                     DLKEKACP"
     gene            53596..54653
                     /locus_tag="AC06IO_000120"
                     /pseudo
     CDS             53596..54653
                     /locus_tag="AC06IO_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(54791..55090)
                     /locus_tag="AC06IO_000121"
     CDS             complement(54791..55090)
                     /locus_tag="AC06IO_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873341.1"
                     /translation="MERFIFKGTSLLKLVLAFSLLIVALILVTPANAKAVNRDINPII
                     NKANPTQMAYWVRYRYWGPRYYRGYYYRPYYYRWGYPYYRPYYRPYYRPYYYYRW"
     gene            complement(55218..56042)
                     /locus_tag="AC06IO_000122"
     CDS             complement(55218..56042)
                     /locus_tag="AC06IO_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873342.1"
                     /translation="MNEKLIETQSNLIKQAETELIQSNQLLESLQQEKGLQEESDLDE
                     LDSLKSLILVLQYRLDTQNALSQLGHLQQYLLQLLGIEAKHSFEFNLERLAFALGTDE
                     LKQILAMLHHLVDSLLRVIAQKQLKESHDKRKSLEQTKQFKPIDTLVKVLNKQKLFMA
                     KLEELKLVLENIGGAPQPGIIYDHIAALQGPISRFYQALQHGLGLSNSLYQQLQKKNQ
                     LSYQLPDTLLKPEHVIRNMNFHPESQRLFKTTLEHNKIENLEERAAAKRLGHFFNH"
     gene            56163..57287
                     /locus_tag="AC06IO_000123"
     CDS             56163..57287
                     /locus_tag="AC06IO_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIO9873343.1"
                     /translation="MEQASAQIVFEQKNATYQCCGSWSVLTLEGLPKKFITGSLPDSQ
                     QITINGEKISAFDSAGALALKKCIDELKKRNNQIELVNFSDSQQQMLELIESKQDILS
                     YQLPAVSKEGVLYQIGKESENKLHQVDGLIILIGDLSTRLFEAFAYWRRFHLPSIVSV
                     IDSAGLKALPIIGLLSFLIGVVLAYQMGLQLETYGANIFIAYLSGMAIFREFGPLITA
                     IIVAGRTSSSFTAQIGSMKINEEVDAILTMGLSPTELLVLPKVLGLLIVFPLLIFWAD
                     VFSIWGAMIMSKNMLNIGFEDFLSRLRDSVGLNQMMLGLYKAPAFSILIALVGCFQGF
                     RVESSADSIGSQTTKSVVQALFLIIIADAAFSIAYSWMGL"
     gene            57288..58025
                     /locus_tag="AC06IO_000124"
     CDS             57288..58025
                     /locus_tag="AC06IO_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9873344.1"
                     /translation="MSEPVIEIKGMKNCLGGQWVHTDINLTVERGEILAIIGGSGSGK
                     TTILRSLLMLLKPSAGSIKVFGEEIYNLDAEQAFRLRRRWGMLFQHSALFSAMTVLEN
                     VMFPMREFTTLDQDFMQELALLKITLVGLSKEAAVKYPSELSGGMQRRAAAARAIAMD
                     PELLFLDEPTTGLDPRGARLFDDLVVFLRDALNLTIVMVSHDIESLKRATDRVAFVGD
                     GKILSVEPINELMKNKNPLIADYFSKF"
     gene            58055..58978
                     /locus_tag="AC06IO_000125"
     CDS             58055..58978
                     /locus_tag="AC06IO_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIO9873345.1"
                     /translation="MESKTNYTIVGLAVLILTAGLLSAGLWLSVGFNQKEYTSYTVYL
                     KESVSGLSIESPVKFNGVQVGYVKEIKLNKNDPRQVELLLNIEKSTPITTSTSATLIS
                     QGITGVTYVGLSAGSSELTPLRKMPGEPYPVIPSKPSLLNQLDAVLKEVAENVGAVSE
                     KAQLIFNEENADNVRKSLANIERITEIIADKGQTIDSSLNNLDVFVANMAKASKQFPQ
                     LIKDLKTGISKFKSLADNMSAAGKDVSKTMIAGKNTIDQISQQAIPPAVILLRRLNAI
                     SANLEKVSNEMRQNPSVIIRGTKAPKLGPGE"
     gene            58982..59584
                     /locus_tag="AC06IO_000126"
     CDS             58982..59584
                     /locus_tag="AC06IO_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HIO9873346.1"
                     /translation="MRVLSSVCFILGILTLMSCSPVKVPVTNEYQLTAYSTKQLTRKP
                     RAITIQVTVPEAVAGYQTEEMLYMKKPFKLEPFVKNAWTSPPADMLFPLLVQSLQSSG
                     YFYAVTSSPYSEEADYRLDTQLLKLEQNFIKKPSVLEFSAKIVLTHISDNQIIGSRIV
                     SLQIPCSQDTPYGGVIAANQATFRFTATATDFVVSHIKRD"
     gene            59682..60212
                     /gene="pal"
                     /locus_tag="AC06IO_000127"
     CDS             59682..60212
                     /gene="pal"
                     /locus_tag="AC06IO_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIO9873347.1"
                     /translation="MKAGSFYKLGLLVASAVLVAACSKTPGSADGGAAVGDGDATAQG
                     LGQMTHFAGQEPGESYTTQAPHNQLYLFAYDDSTLASKYLPSVNAQAEYLKTHPGARV
                     MIAGHTDERGSREYNVALGERRADTVAEILRMAGVSRQQIRVVSYGKERPANYGHDEA
                     SHAQNRRVEFIYEATR"
     gene            60209..61189
                     /gene="ybgF"
                     /locus_tag="AC06IO_000128"
     CDS             60209..61189
                     /gene="ybgF"
                     /locus_tag="AC06IO_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIO9873348.1"
                     /translation="MINRKKSIITLCFVLMLPFASWAEAPVIDDSENFAMIDRQEEYD
                     APLVNPKYDNPQIESAELDGSQNDNYSTDTSQSYDEPALVKEDRSTISDNAKLIDKIQ
                     QLQKEIQELRGQLEVQAHDLKLLQQQQVAFYKDLDSRLSNSSTSAKTIQNDKPATDVS
                     LGSNSPATLKAASPQIKAGPSTGPSNSKPQPVIAVSRANPADEQISYLAAYELVKNKR
                     YDEAIKSMQTFVQKYPRGGYTANAEYWLGELYLVKKDYPKAIEHFEIVLQQYPSSSKA
                     AASLLKSGYAYAEKGDTQEAKKRFQQVVKTYPDTPTAQLASSKLEAINAL"
     gene            61186..61839
                     /gene="queE"
                     /locus_tag="AC06IO_000129"
     CDS             61186..61839
                     /gene="queE"
                     /locus_tag="AC06IO_000129"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HIO9873349.1"
                     /translation="MKRFNEQLRITEIFHSLQGESVTVGLPTVFVRLTGCPLRCQYCD
                     TAYAFSGGEVVEIDDILNKVASYQCQHVCVTGGEPLAQPGCIPLLSKLCDAGYSVSLE
                     TSGARDIASVDQRVMIVMDLKTPDSREADKNLLSNLSFLKPTDQIKFVLCSRTDYEWA
                     CSMLSEYQLAERVQLLFSPSWNQLNPTDLANWIIQDKLPVRFQLQLHKILWNDAPGH"
     gene            61857..62804
                     /locus_tag="AC06IO_000130"
     CDS             61857..62804
                     /locus_tag="AC06IO_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate/mevalonate 3,5-bisphosphate
                     decarboxylase family protein"
                     /protein_id="HIO9873350.1"
                     /translation="MHWFAQAPANIALIKYMGKKDENSNLPDNSSLSYTLSNLLSSVK
                     LEKLPTKKDIWEPLTIPGAPEFNLSVEAQKRFIDHLVRLKEYFGYVGGFLIQSSNNFP
                     HSSGLASSASSFAALTKCASIALSELTQKPLPSIDEQAQWSRLGSGSSCRSFYAPWAL
                     WTGDKVSAIDLPYKDLLHQVIVISSQEKEISSRVAHKLVKTSPFYETRSERAEANLKL
                     LLNAFENKDWTSIYQICWHEFLDMHQLFKTCEKPFSYITDNTLHILSVIEKFWNEKGD
                     GPVVTMDAGPNVHLLYRSDQTDLARQFKSDHLVGNYDVL"
     gene            62794..63672
                     /locus_tag="AC06IO_000131"
     CDS             62794..63672
                     /locus_tag="AC06IO_000131"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase family protein"
                     /protein_id="HIO9873351.1"
                     /translation="MFYDFETTTFGKWILAGEHAVVRGHEALVFPIKERQLTLKYNPS
                     SPSLSAEFQGINGSDMHLLFWSVLERGMHLLGRSLNELVGHFYLETNIPVGVGMGASA
                     ALCVAMSRWYCAQQMIQDNQCNEFAKQLEHLFHGKSSGLDVAGVASDTGVYFKSGLCT
                     PVKQTIYPYWYLSSCNQIGITSHCIQQVEYLWNKNPELAHQVDKQMVEAVQEAKAVLE
                     EGGSNALNRLAKAINKAADCFLQWGLVSESLQQHMNHLFADGAIAVKPTGSGGGGFVL
                     SLWDKIPSSHIELIPV"
     gene            complement(63685..63954)
                     /gene="ftsB"
                     /locus_tag="AC06IO_000132"
     CDS             complement(63685..63954)
                     /gene="ftsB"
                     /locus_tag="AC06IO_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HIO9873352.1"
                     /translation="MRPIFIILIVALVALQHKLWLGDGNIIQWIKLEKKLEAHKSQND
                     KLAARNKALEADIKELKSGDQALEEQARYELGMIKQNEVYYQFVD"
     gene            complement(64176..65444)
                     /gene="eno"
                     /locus_tag="AC06IO_000133"
     CDS             complement(64176..65444)
                     /gene="eno"
                     /locus_tag="AC06IO_000133"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIO9873353.1"
                     /translation="MHIHKIQAREILDSRGNPTIEADVILENGIIGRASVPSGASTGS
                     REACELRDNDPKRYAGKGVQKAVKHVNEEINQALQGLSVGDQENLDRILCQLDNTENK
                     SHLGANAILATSLACARARALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVD
                     IQEFMIMPIGAPDFPVALQMGTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQAL
                     DLLSEAIEKAGFRLGEDIVFALDVAASELFNEGFYHMSSENQKFDSHQLIEYYANLIS
                     SYPIVSIEDGLDEKDWSGWKQLTTLLGNKVQLVGDDLFVTNPKILQEGIAQGIANAIL
                     IKVNQIGTLSETRQAIKLAYDNGYRCVMSHRSGETEDTFIADLAVASGCGQIKTGSLC
                     RTDRTAKYNQLLRINELASLPYAGKNILKR"
     gene            complement(65476..66078)
                     /locus_tag="AC06IO_000134"
     CDS             complement(65476..66078)
                     /locus_tag="AC06IO_000134"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIO9873354.1"
                     /translation="MSKFLQQKPIILASSSTIRHKLMKSLGLDFLVVPSNCNEEEIKT
                     RHNSDELVELGITLAKIKALDVSQHYPEHYIIAADQLCIADKRIFNKPLNHQTAVSHL
                     RELSGKQHQQIACLCIVKESKILWQYHETATLTLHHLSEKTIEAYLQAEKPYQSCGAY
                     QYEGLGKWLFKEVQGSEDTILGLPLMPLVNALVNLKVVGI"
     gene            complement(66071..66829)
                     /locus_tag="AC06IO_000135"
     CDS             complement(66071..66829)
                     /locus_tag="AC06IO_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIO9873355.1"
                     /translation="MLSATSLKIIFAISIFIVILIAGWYPFKKRLKDDKHIDFPIGET
                     LATGVFLGAALLHMLPESNTLFKEMGYNYPFAFIITGVVFLIFLWFEHLGKELYHHHD
                     AEHPAFAILAWAMLSVHSLMLGAALGFTQYNSMIIMLFLAIITHKWAESLAIAIQLNK
                     SSMSTRKSMVFFFFFSLMTPLGIYFGWYFGHGVETNSLFDPILIAASAGTFLYLGTLH
                     GLERCVMVERCCNLSDFSFVIIGFLLMASVAIYV"
     gene            complement(66945..68087)
                     /locus_tag="AC06IO_000136"
     CDS             complement(66945..68087)
                     /locus_tag="AC06IO_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIO9873356.1"
                     /translation="MYPHDRYWQAKKVTLIGAFINALLGVVKMIGGFLYHSHALIADG
                     VHSISDLITDVMVVFASKYGSVSADDSHPYGHQRIETAATLLLSLLLILAGCGIAWDA
                     LDELLKSDHTMPNWLSIPIVCFSIIANEALFHYTRHVGKKISSQLITANAWHHRSDAA
                     SSIVVLIGLIGSLAGLVYLDAIAAVIVSLMIIKMGWSYGWNSVKELVDTAVDAELFSQ
                     IERVIQSIDGVQRIHQLRSRFMGSDVLIDVHILVSPKISVSEGHYIAQHVHHTLVKEI
                     DCVKDVIVHVDPEDDEISCPSLHLPNRAILQEKLFNDIRHDFPQILFWNIHYLNGKMS
                     LDIFCSNDFNQWKKLHNRVISTLKSQIDIKEVRLFGLHEEMHHLSN"
BASE COUNT        19822 a        12943 c        15086 g        20385 t
ORIGIN      
        1 atattgagct atagtcaata gataaatgaa ttaatttatt aaccaaacac cgtggaatgg
       61 atatgaacat caaagagaaa attggccaaa gaattatgaa tgaacgaaag gctaagggat
      121 taacgagaaa agcattggct gaattgacag gcgaattgaa aacctcacgc attaataatt
      181 atgaacgagg ggaccgtacg cctggtccaa ctgaaattaa attgctggcc gatgccctgg
      241 aagtctcagc ctcttatttg atgtgtttaa ctgataatcg tgaagggaag atgacaaaat
      301 cccttggcat gggcgcattg attcctgtct tagattacaa acaagcagct gatcctgtta
      361 cttatattca gaaaataaaa gatgatatcg atagcaaagt tgagtttgta ccggtcagta
      421 aagctgttgg tgaaaaaatt ggaaaaaatg cttttgcttt acaggttagg gatgaaagta
      481 tgatgcctga atttagagtt gatgatgtga ttattgttga tcctgataca tgccctaagc
      541 caggagattt tgtgattgca ttggttgaag gtgaaaatga agtgatagtt cggaaatata
      601 gacaattgtc cctttccaag aaggagcaag agttcgagtt ggttgcactt aataatgatt
      661 gggctgattt acgcgttgat gctagcacat ttaaagtgct gattgttggt agtgggataa
      721 gcttatgtcg ggaactaaga gattaaattt gctgttttta ctataaaact cttggaagta
      781 tttgagactg tccgaaaaaa ctcaagaaaa ccagcctact ggttgtcatg gaatcaccag
      841 gtgggataat cagcatgtat acttacaact ttcatctctt aatttattat tgggttacta
      901 gaattccaaa cggcacgaag agtgtttttc cattgatttg tacttgggca aacattttaa
      961 taaaaccagc tttatcgggc tcaatatgaa attgcaattc aggtccacct ctttcagagc
     1021 catttgttgg ttctttaccc attggatgga catgggttac tgttttaaag tcttcattaa
     1081 aaccaacgat atgtgcataa gcacccataa tcggctctag ggtatgaaca ggctttccat
     1141 tagcatcgac aatatcaatt ttgcccatga tgggttgacc tacgtgtaat ggtgttttat
     1201 caaaagttaa tttgaatttg taaccatcaa cggtactttc aaagaatggt tgtctaacca
     1261 ttcgatcttc aattctcttt atgatttttg gagagggaaa atcggcaaca acgtattctt
     1321 ggtttttggt attcgcaggt attagatcag cccatgctcg ataggaggca tttttcaggg
     1381 taggctgcca ttcaaattga taaacgccgg gtatttttgt ctctattgga tgaacatggc
     1441 tgtagtcaac gaggtggtca tcaataatca acaaatgaat tttctgagta tgggcctcaa
     1501 tgagattgcc taaggtgatg ggtttattgt ttttagtatc gcttaactta atcaatacta
     1561 attttttatt ttctttatcc atgacttctt gaattgataa ttgcatggta ggacgagcct
     1621 ctgattgtga agactctaac gagacatggg tcattgtgtg atggtgggaa ttctcagcaa
     1681 aagcaatagg atgatataaa ttgattgatg ataagctcat taaggtgatg gtcaattttt
     1741 tcataaaact tgggtctcca aaacaatcat ggatgtatta gtatcattgt ttataatgat
     1801 tttgtttgtt attgattcaa atcagtaagt acaatgatat catggtttga ttcataggcc
     1861 atcaacagcc cccatctgct ttgatttatt gtgtgaaacc gtctatattg tagtcatatt
     1921 tccttttaaa atcatgggga aagaccttca tgaagcaacg tcattggata attttagctt
     1981 gttgtttagc acttgctgcg tgcatgaata atggtacctc gactaaaaat acagagagtt
     2041 tagatagtca tgtggctcaa ggccatgttg ggcatggtgg cggtggtggt cgctaaccct
     2101 atagtttttg gattgtaata caacggaaag ccatcagtga tgctgtaatt aatatgaggt
     2161 tactttagga agaattaagg tcatttgaaa atactggctg agcggattca aaggatgaat
     2221 tacaatggca gaacaatggc atgaaaaaac gattcaggat atcgcatccc agtttaaaat
     2281 agacctgact gtaggtttaa gcgaagcata tgccacagaa cgattaaaag aagtggggcc
     2341 aaatttgctg agtcaaaaga aaaaaacatc agcactcatt atcttctttc agcaatttag
     2401 caatatagtc atctgggtgc ttttaggtgc tgttatcgtt tcttttttac tcggcgaaaa
     2461 agccgatgcc attgccattt ttgccattgt tattttaaat gccattattg gtttcgctct
     2521 agaatatcga gccgatcgcg ctatggcagc actccaggaa atggccgcac caaaggcgac
     2581 tgttttgcgc gatggccatg ccaaaatgat tgctgcctcg gatatcgtcc caggagatgt
     2641 tatccttttt gaaagtggtg atttaatcgc tgccgatgcc cgtcttttta agttgtcagc
     2701 actcaaggtc aatgaagcac cgctcacggg tgaatccatg ccagtaggta aaaacctgga
     2761 cctttgcact caagaaaccc cattagccga ccgtaaaaac atggtgttta tgggaacggc
     2821 cattgccgat ggtacgggac gcgcactggt tgtagcaacc ggcatgcaaa ctgaaatggg
     2881 tcgtattgcc cagctgcttg gtgaagcatc tcgtgataaa acccctttgc aaaaaaaact
     2941 caatcaagtg ggatctcgtt tattatgggt ttgctttttc attgttatgg ctatttttgg
     3001 cttaggcctc ctgcgtaata ttcctatctt caatctgttt atgagttcag ttagtcttgc
     3061 tgttgctgcg attcctgagg gtttacctgc tgtggtcaca gtggctttag cactgggtgt
     3121 acaacgtatg gtgcggcgtg ctgtgttggt aagacgatta tcggcagtag aaacacttgg
     3181 ctatttacaa gtcatttgca ctgataaaac aggaacttta actgtgggtg agatgacagc
     3241 acgtaaattt gtgacagcaa gcgatgtata tagtatttat ggagagggct ataatctcac
     3301 aggaaaattt tcacttcaag gccaggagat taatgtttca gaagacaagt tattgcaggc
     3361 ctcattacag gctatggtag cctgcaataa tgctgaattt cgacaacaaa atgggcaaat
     3421 gacaatagca ggggatccga ctgaggtagc tcttttggtt gcagcagcta aaggaggttt
     3481 ttggcgaagt gaactgcaaa tttctaatcc tcgcattaag gagctgcctt ttagttccga
     3541 acgcaaacga atgacagtgg tttgtcaata tgataatgat gagctcatgg tttacgtaaa
     3601 aggggctcca gaaattgttt tagaacgctg cactcatatt ttaacaaaaa ctggtattaa
     3661 aaaattaacg ctgaatgata aagcgcgtat gagacaatcc tgtgaattca tggctagtga
     3721 agcattaagg attttggcat tcgcacaacg cccattggat acttcatggc tggataaaga
     3781 agttgatgag attgaaagca atttcgtttt tttaggtttg gttggtctgc aagatccacc
     3841 gcatgccagt gcaagagaat ctgtttcccg ctgtaaaaag gcgggtatca aactggtgat
     3901 gattacagga gaccatcctg ttacagcgag agccattgct caagaacttg ggatattaag
     3961 cgaaggtgat caactcctta ctggcaatga acttgaaaac atgtcagagg aagaatttaa
     4021 ctattgcgtc aaagacattg tggtctatgc tcgtgtgact gcggagcata aattaaaaat
     4081 cgtacgtgct tggaaaaaac aaaacagagt agttgcgatg acaggtgatg gtgtgaatga
     4141 tgcgcctgct ctaaaagaag cttctattgg tattgcgatg ggaaaaacag gagcttctgt
     4201 taccaaggaa gcttctgaca ttattgtcat ggataataac ttcacatcaa ttgtagcagg
     4261 gattgaagaa ggtcgaacga tttacgataa tattatcaaa acactcattt atttgttagc
     4321 aggtaacagt gctgaattgc tggttgtttt tgtagcgctt ttaatcggat ggcccttgcc
     4381 tttactccct attcaattac tgtggattaa cttagtgact gacggtttac cagccattgg
     4441 tttagcaact gataggtcag agccaggaat tttgaattgc ccacccagaa ccattcaaaa
     4501 atccatgata gatagagcgt ttctcaaacg agtgagtttt attggttgtg taacagcttt
     4561 agtaacttta actgtttttg cttatgaata tttagcttat aaggatttaa ctcaagcgca
     4621 agatgctgcg ttttccatat tagtgacagc aagacttttg atagcatttg gagctcgtag
     4681 tgatactaag actatctttc agctcggctt tttctctaat ctgcatttat ttataattat
     4741 tagcattagt ttttcactac aggtcttgat tcatcatatt cccgtactgc gagatttgtt
     4801 tggcattggg caaatctcat ttacccaatg tatttcctgg atcttgatag gcacgttgcc
     4861 tttgttagta attgaagctc aaaaatggct aaggaaagca cctggtgaga cattttagta
     4921 ttagaaaaat tatttggatt gttctcctga gtttctgctt tacggagtca ttcatttatg
     4981 caaagcccaa tcctggttta tcgcagttaa ttcaggaagc taagcaaaac aatccccaaa
     5041 ttcgggcggt acgcgaccgc ctgcttgctg caattcacgt gattcctcaa gcgaaagccc
     5101 ttcctgaccc gaaattgaac gcgggttata tcaatatgtc cgagaacatt cctatggatg
     5161 tcgatccaag acgtgaacaa atgttgggtg gccaacagga aattcccttt cctggcaagc
     5221 ttgttgtgcg aggaagaata gcaacgcttg aggccaaacg ggcagaagcg gagtatcaag
     5281 ccacttgttt tgctgtcatt gctgaactaa aacggctcta ttatgacctt tactttgtca
     5341 ataaatccat cgagattgtg caaagaaatc aggagcttct ccatgaaatg gagcaaagtg
     5401 ccgaagcgac gtacagtgtg gggaaaacgc cccaacaaga catttatcgc gcccaaacgg
     5461 aagtttctcg tctagagatg cgtttggtca tcttaaagca acaacgccaa tccctgcaag
     5521 ccgatatcaa ccgtcttttg aatcgttcct tagaaagggt ggtcagtact ccaacaacac
     5581 tccctgtcac accaatgggg cacaatttgg agtattttta ttcgctcgtg aaacagcgag
     5641 cgcctcaatt aatcatgcag cagcgtaacg tgcaaaaagg acgacaagcc atcaagttaa
     5701 gtaaaatgga gtatttcccc gatgttgaaa ttgagggggg acgacttcat gatacgggaa
     5761 tgcataccaa aggttatcaa gtgttgttaa aagcaaccgt tcccctttat ttcatgcaaa
     5821 aacaaaataa tgccgtacgg gaatcacttg ctcgttacaa cgccgatatg gaagacttgc
     5881 agacgactta tcgcacactt tcttttcaag taaaaaatgc ctatttatta gcagagcgct
     5941 cggcaaagct tattcatctg attcaacata ccattattcc gcaagccact ttaacgttta
     6001 cttcttctca agctaattac ggggttggca aagtggattt tttaaccatg ctaaataatt
     6061 tgttgaccct gcaagaaaac gaattggagt ggcacggtga actcgctgag catgagaaag
     6121 cgattgcaca aatcgaagaa agcacaggga cgtacttatg aatcagaagg tgatgagaag
     6181 gggtgtgttc gttttgagtt tactggcttt gttgcagtta acccaggcac aaaatgcaac
     6241 ggatacacat agcaagatgg acatgaaaca atccacgcaa aaaaatgaga ttgtgattga
     6301 tccagtccgc ttacaagcca ttggcattac ctcagagccg gtacgccgtc aaaacattga
     6361 aaaaacaatt cgcacggtag gaagggttga agccgatgag cggcgcgttg cccacattca
     6421 tgtgcgtttt gatggttgga tagaacaatt attcatcaat ttcaccggcg aaaaagtaca
     6481 aaaaggacaa gcccttttta cagtatatag cccggagttg gtggccaccc aacaagaata
     6541 cttacttgcc ttaaacgctc aaaaaatctt aagcaaaaat accacctcca gtgccgcccg
     6601 tggtgcccaa ggggcctttc aagcggctca tcagcggttg ctgttgtggg ggatttctga
     6661 aaaggaaata gagcaattaa agcgtactgg caaaattact cgaacgatga ccattgactc
     6721 acccatccaa ggtacggttc ttaaaaaaat ggcgcttatc ggcatgcgta ttgagccagg
     6781 agatgaattg tatacgattg ctgatttatc acgcttgtgg gttttagggg atatttatga
     6841 atacgagttg ccctatatta aactaggaca aactgccgac ctgacgctta cctatttgcc
     6901 caatgaatta tttaaagcca agcttgattt tatttatccg accattgata tgaaaacgcg
     6961 aacggccaag gtgcgtttcg aagtagacaa tacaaaagaa caattaaagc ctggcatgta
     7021 cgttaatctg gaactcaaaa tccctttggg tgaacgtctt gtggttccta aaaatgcggt
     7081 tttgctcacg ggtgagcgag cggtggtctt tatttatcat ggcaatggca aaattgagtg
     7141 gcgagacgtc actcttggag tgcgggccgg tgatttgctt gagatagtaa aaggggttaa
     7201 ggagggagat aagattatta cctctgccaa tttcctcatt gattcggaga gccagttgaa
     7261 agcagccatg ggaggcatgc agcataaata ataagagatg aattgcttat gagtgttatc
     7321 tcgtaaggct cttcctttga agaacaggga tgatgtatga gattcctatg aaataaaggg
     7381 attttatggt tgaacggatt attgaatttt gtgcgcgcaa tcgctttatt gtgttgctct
     7441 ttgtgcttgg gctttctttc atgggttata tggctcttaa gaatacccgc atggatgcat
     7501 tacctgatat ttcagacacg caggtgattg tgtacaccac ctggatggga cgctctcctg
     7561 atttaatgga agaccaagtc acttacccta ttgtgacagc actgctgtcg gcaccgaaag
     7621 tggttgcggt acggggattt tctgattttg gcttttctta tgtttacatc atttttgagg
     7681 acggtactga tatttattgg gcgcgttctc gtgttttgga gtacatgagt caactggcag
     7741 gaaagttgcc cgaaggggtg acaccgcaat taggccctga tgcaacccct gtaggttgga
     7801 tttatcaata cgccttggtg gatgagaatg ggaaacataa tctggctgag ttacgcacgt
     7861 ttcaagattg gtatttacgc tattggttaa gagcagttcc tggggtgtct gaagtagcca
     7921 gtgtgggtgg gtttgtcagg cagtatcaag tcaatttgga tcccgtcaaa gttcttgcct
     7981 acaacctgtc ggttcctgac atcatcgaaa aaattcgcat gagcaataac gacaccggtg
     8041 gaagggtggt tgaattttca ggcgtggaat atatgattcg tggccgagga tacattaagt
     8101 ctaccgagga cattgaaaaa attgccatag gcactaatgc caatgggaca ccgattttgc
     8161 tgcgtgatgt ggcgaccgtg cagcttggct cggacatgcg ccgaggggtc gtcgatttaa
     8221 atggccaagg agaagcagtg ggcggcattg tcatcatgcg ctttggtgag gatgtgttgc
     8281 aagtgattgg tcgcattaaa gagaaattaa aagaattggc cactgccatc cccgaaggaa
     8341 taaaaatggt tcctgtgtat gatcgcagta atctcattcg agaagccatc tccacggcca
     8401 attggaattt gattgaagaa ctggtggtgg ttgggttact catcatcgta tttttaggtc
     8461 attttcgttc cgcattaatc cccattatta ccttgccgct tgcgattttg atttctttta
     8521 ttcccattta tttcttgcat attgggctta atatcatgtc cattggtggg attattgtgg
     8581 cggttggcga catggtggat gctgccatca tcatggtcga taatgcccat aaacgcttag
     8641 ccgattggga ggcaaaagga agtccaggcg atcgtaccca agtgttaatc gattcggcca
     8701 aggaagtagg tcctgcgatt ttctcatcac ttcttgtgat tgccatctcg ttcatgcccg
     8761 tttttactct agaagcccaa gagggacgga tgttttcacc tttggcttat actaaaaact
     8821 tggcgatttt catgagtgcc ctgttggcca tcaccctaat tcctgtacta ttgcctcttt
     8881 tggtgcgtgg tcgaatcatt cctgaacaaa aacatcccat tacccgcttg atgcaagcaa
     8941 tgtacgcacc cgtgttaacg ctcgctttaa gctatcgaaa ggttgtcgtg ggcgttgcca
     9001 tagtggtggc attagccaca atccctctct acaaactcat tggcgctgag tttatgccac
     9061 ctctttatga aggcacgatt ttgtacatgc cagtgacttt gccaggtatt tcagtgactc
     9121 aagcaacggc tttgctgcag gaaatggata agaaattaaa agcctttcca gaagtagccc
     9181 atgtgtttgg aaagacagga cgagcggaaa cctctaccga cccctcgcca tttagcatga
     9241 tggaagtgat tgttgaatta aagcccaagg aattttggcg taaaggaatg acttatgaag
     9301 gcttggtggc tgaaatggat aaagccttgc aatttcccgg ggtgagcaat gcctggacca
     9361 tgcctattaa agcacgcaat gacatgctca ccacggggat tcgaacggcc gttgggatta
     9421 aaatttttgg tcccgacatt aaaaaaattg aagccattgg caaagaaatt gaaatggtgg
     9481 ctaaagaagt gaaagggacc agtaatgtct atgcagagag agtcgctgga ggttattttc
     9541 ttgattttca aatcaatagg gaccagctgg cacgttacgg tttaaccatt atggacgtga
     9601 atcgcattat tgagtcggcc gtgggtggtg aaaacattac taccacgatt gaagggcgtg
     9661 agcgttatcc ggtgaatgtg cgctatttgc gggagctacg tgacgaccca gataagctca
     9721 atcgtatttt agttaaaaca ccaagtggag ctgaagtacc tattgcacaa ttagttactt
     9781 taacatttcg ttcgggacct gccatggtcc gcgatgaaaa tgccatgttg gcaggttatg
     9841 tgtttattga tatcagtggt cgggatattg gtggctacgt ggaggagcta aaacaagcag
     9901 tcaaggccaa agtcaaattg cctccaggct acaccctggc gtggtcaggt caatacgaat
     9961 tcatgcaacg ggtttatgag cgacttaaaa ttttcgtacc actcacctta gtcattattt
    10021 ttgttttatt ttattttacc tttcgtacca tcacggagac tttgatggtg atgctcggtg
    10081 ttccttttgc tttatttggc ggctttttgc tgctttatgt tttgggatac aacatgagta
    10141 ttgccgtttg ggtgggaatg attgctttag ccggtgtggc cgccgaaacc agtgcggtga
    10201 tgctggcata ccttgattcg gattacaatg agcaaaaaga gaaagggttg cttaaaaccc
    10261 tgccggattt aattcaaatg gtacaccttt gtgccatatc ccgtatccgc cccatggtga
    10321 tggcaggtct tgcgaacatc attgggttat tgccggtgat gtgggccacg ggcattggag
    10381 cggatgtcat gaaaaggctt gcagcaccta tggtgggtgg tgttttttcc gcccttttac
    10441 tgactctcat tgtcattccc gtggtttatg tgatgtggcg ttggcaagtg gaattgaaac
    10501 aacaaggaag tctgacggag gcaaaatgaa attaaccttt ttaggggcaa cagagaccgt
    10561 taccggatca aaatatcttt tgacggtaga ttctaaaaaa attcttattg attgcgggct
    10621 ttttcagggc tataaagaat tgcgcttgcg taactgggct cccttgccag tcaatccacg
    10681 cgacattgat gcagtcatca tcactcatgc tcatattgac catacgggtt atcttccctt
    10741 gctcattaaa aatggatttc aaggcaaaat ttatgcaaca acgggcacca aagcgctgtg
    10801 ttctatttta ttaccggata gtggtcatct ccaggaggaa gaagcacgat tggctaacaa
    10861 atatggattc tccaagcata ggcctgctct tccactgtat actgagcagg aagctcgagt
    10921 tgccttaaat tattttgaga ccattgattt tgatacgcca cattcattat ttaatgtgtt
    10981 gagttttgaa tttcatcgtg ccggccacat cgtgggggct gccatggtca aaataaaaac
    11041 aaaaaaaggt tccatggtgt ttaccgggga ccttggccgg cctcatgatc ccgtcatgaa
    11101 agccccaagc gtgattcaag aaaccgatta tttggtgatg gaatccactt acggagaccg
    11161 gctgcatgat gccaccgatc ccttgccaca aatcggtgag gtgattaatc gcaccatcaa
    11221 acgaggaggc tctgttatta ttccggcctt tgcggtggga cgggctcaaa gtcttttata
    11281 ttttatttat gaattaaaac gcaaagggga aattcctaaa gagattccag tatttttaga
    11341 cagtcccatg gcgattaatg caaccaaact actctgtact tataaagagg atcatcactt
    11401 aaatgaggaa caatgtcgtg gattatgcaa tgtggccact tacattaata cgcctgagga
    11461 gtccaaagag attgacaggc ataaaatgcc acaaatcatc atagccgcca gtggcatggc
    11521 tcaaggcggt cgtattttac atcatttgaa agcctttgcg cctgatccta aaaacacact
    11581 tctttttact ggctttcagg caggaggtac tcgtggtgcc aggatggtta atggggagcg
    11641 ggaggttaaa attcatggtg gtttggttcc catacgcgct caggtggtgg ttatgagcag
    11701 tacctctgct catgccgatt atctcgagat gttagagtgg ctaaagcatt ttactcatcc
    11761 gcctaagaag gtctttatca cgcatggtga actccacagc gctcaatcat taaaagagaa
    11821 aattgaaagc caattaggtt ttccttgtac gattccttct tatctgcaag aggaggattt
    11881 gtcgtgatcc agaaggaaca tcataggttg cgtttaaagt acctaggaat taaaacatat
    11941 catgaagcca ttatctacat gcgagaagat tgccatgttt gtcattcgga gggatttgag
    12001 gttcaaacgc gcatccaagt gaccttgggc cagcgctcga ttattgccac gctcaatgtc
    12061 gtgacctcag agcttttaag gcctggtgaa gcgagcttat ccaattttgc gtgggatttt
    12121 ctcaatgcca aagaagggga tgacattcaa ctgtcacatc ctaagccact ggaatcatta
    12181 agttatgtgc atacgaagat ttatggtaat gaactctctt ttaaacaaat caaggccatc
    12241 gtggatgacg ttttaagtgg ccgtctttct gatttgcaaa tttcagcttt tttagcggcc
    12301 agcgccgcgg aacgtttaag caatattgaa atcgtgaaac ttacaaaagc catgattgac
    12361 agtggcgatc gcttatcctg gtcttcccct ttgattgtag acaagcattg tgttggtggt
    12421 ttaccaggaa atcgcaccac ccttgttgtg gttcccattg tagcggcttt cgggttgacc
    12481 atccccaaaa cctcttcacg ggctatcact tctcctgcgg gaactgcaga tacgatggaa
    12541 actttagcgc cggtgtcact tagcccagag gctatgtgtc aagtggttga taaggaaaac
    12601 ggatgcattg tttggggagg ggctgtgagc ttaagccctg ccgatgatgt gcttattcgg
    12661 gtggagcgtg ctcttgagtt agatagtgaa ggacaattgg tcgcctcaat cctttctaaa
    12721 aaaattgcca caggagcaac ccatgcggtt attgatattc cagtgggacc taccgccaaa
    12781 gtcaggaata agtccagggc tttacttctt aagcaatccc ttgaagaagt aggaaaacag
    12841 ttaggtcttg tggtccatac cttaattact gatggcgcac aaccgatagg aaatggtatt
    12901 ggcccggctt tagaagcacg cgatgtgttg gcggtattac aaggtttgcc tcatgcccca
    12961 aaggatttgc gtgaaaaatc attaactctt gcaggagcag tactggaatt ttcatcaaca
    13021 gtaccaccag gctcaggtca atcgatagcc aaacaattat tagatagcgg agaagcgttt
    13081 aaaaaatttc aggctatttg tgaggcacaa ggagggatga gggaattgac ggtggcacgt
    13141 tttactcacc ctatggttgc tgagaaagca ggtaaagtat ctctcattga caatcgaatg
    13201 ctcgcaaaaa ttgctaaact tgcaggggcc cctaagtcta aatcagcagg ggttgatttg
    13261 catgttcatg ttggtgacca agttgaaaaa gggcagcctt tgttcaccat ccactcagag
    13321 tcttcagggg aactgactta tgctcgcgat tttattcgcg ataaacaaaa taccattgtt
    13381 ctaggagaga agccttgaag cccattttat tttcattatt tgatgcttca ggaatcagta
    13441 gacgcttaaa agaagcgctt caagtggagg tgggtgatgt cacgttgcat tgttttccag
    13501 atacagagtg gtacgtaaaa attaattcag cagttaaaaa tcgcagcctc attgtagtgg
    13561 atagcttaaa ccaacccgat ggaaaaatac tgcctttgct gttttttgct aaaacagcaa
    13621 aagaattagg agctaaaaaa atcggcttga ttgcccctta tcttccctat ttacgtcaag
    13681 ataaacgttt tcatcagggg gaagggatta cctcgcgtta ttttgctgaa ttactctcta
    13741 cttcatttga ttggctcatg accatcgacc cccatttgca tcgttatcac tctttggatg
    13801 aaatctattc aatacctact tttgtcttgc atgttacagc caatattgcc acatggatta
    13861 agaatcatgt gaataagcct gtgattattg gtcctgatat ggaaagtcag cagtgggttg
    13921 ccgacattgc tgaaaaaggc catttccctt atgtgatttt ggaaaaaatt cgacgtggag
    13981 ataaagaggt ttctatttct gtgccaacta tccctgacct tgaatcgtct actcccgtat
    14041 tagtcgatga catcatctcc accgccagaa ccatggtgga gacggtgaca catttacaaa
    14101 aggcaggggc tcagtccgtg atttgcattg gggtgcatgc gctttttgca gaagaggctt
    14161 attctttatt atcggggatg gatggtgtgc aaatcatcac gtgcaacacc atccgtcata
    14221 tcaccaatgc gattgacgtg agtgaagtga ttgcaaccac tttagaaaaa tacaaactcg
    14281 ccaaagtgtt aaaaaaatag ggatgaaaaa atggttttat tgacgaagga gcgccaaatg
    14341 tcacttttta aaataatcag ggttatcgga atggtcattg gcttttcggc cattttcttt
    14401 ttgagctctt gtaatcatgc tgtgaaacag agttcgcaag agacggatgg gggctatgga
    14461 gggcacagtg cgggtggaca tggcagtcat ggtggcgttg ggcattaaat aattaaaata
    14521 ggtgactcat gaaacaaaat cctcatcaag gacatcctaa ttcgggtaaa gagcatgctt
    14581 cttgtcatca ggagcataag acccccaagc ctcctgtgcc ttcctccaaa agggaaggtt
    14641 caattgttta tacgtgcccc atgcatccag aaattcgcca gtctgctcct ggtaattgcc
    14701 ctctttgtgg tatggctcta gagccggaaa cagtgatggt ttcggaagct gcgagccctg
    14761 agtatcttga tatgagacgt cgattttgga ttgcgttgat tttgacggtg cctatcgtga
    14821 tattggagat gggtgggcat ggtttgcagc attttatttc tggcgatgtg tccagttgga
    14881 ttcaattact tcttgccaca ccggtggtgt tatggggcgg ctggcctttt ttcaagcgtg
    14941 gttggcaatc tctaaaaaca caccaattaa atatgtttac tttgattgct atgggcattg
    15001 gtgtggcatg ggcttacagt gtggtagctg tcctttggcc aggggtattt ccccaagcgt
    15061 ttcgaaatca ggaaggggta gtcgctgtgt attttgaagc ggcagcagtg attacaacgc
    15121 tcgtcttatt aggacaagtg ttagaactca aagcgcgcga gcaaacgggt agcgccattc
    15181 gtgctttgtt gaaattggct cctgaacgtg ctcatcttat taaagaagat ggccgtgaag
    15241 aagaagtatc gctcgacaag gttcaggttg gagataaatt acgagttcgt cctggtgaaa
    15301 aaataccagt ggatggggaa gtactggaag gacgaagttt tgtcgatgag tctatggtga
    15361 caggcgaacc tattccggtg actaaagaaa caggagccaa agtcattggg gcgacgatta
    15421 accagacggg gagttttgta atgagcgcgt tgcatgtggg cagtgatacg atgcttgctc
    15481 gtattgtcca aatggtgagt gaagcccaaa gaagccgtgc tcctatccag cgattagcgg
    15541 atactgtttc aggatggttt gttcccgcag tgattttggt tgccctattg tcttttattc
    15601 tatgggcctt gctaggtcca cagccgtcat taagctatgg tttaattgct gccgtatcag
    15661 tacttattat tgcctgtcct tgtgctcttg gtttggcaac acccatgtcc atcatggtag
    15721 gggttggaaa aggagcccaa aatggggttt taattaaaaa tgccgaaagc ttggagcgca
    15781 tggaaaaggt caatacgtta gtggttgata aaacaggtac cctaacggaa gggcgcccta
    15841 aattgacccg cattgtgact gacggtgagt ttgatgaaga ggttatttta gggtttgcgg
    15901 ctactcttga gcatcaaagt gagcatcctc tgggtcatgc tatcgtaacg gctgcgaaag
    15961 aaaaggagtt gtcctttggc tcagttgaag actttgaagc acctaccggc aagggagtcc
    16021 tagggaaggt caatggtcat cgcattgcca ttggcaacct gaaattaatg caggatcacg
    16081 gaagtgacga cgcttccctt cttgaaaaag cagatgagct tcgcgctcaa ggtgcctcag
    16141 tcatgttcat agcagtggat ggcaaaacag cagctctttt ggtggtagaa gaccctatta
    16201 aatcgactac tccagaaacc attcacgagc tgcagcaaag tggtatcgac attgtgatgc
    16261 tcaccggaga cagcaaaaaa acagcagaag cggtagcggg tcaattgggc ataaaaaaag
    16321 tggtggctga aattatgccg gaggataaga gccgtattgt aggtgaatta aaagaaaaag
    16381 gtctcattgt ggctatggca ggggatgggg tcaatgatgc cccagcactt gcaaaagcgg
    16441 acattggtat tgccatgggt actgggaccg atgtggccat tgaaagtgct ggcattacct
    16501 tactgcatgg gaatttgcgt ggtattgcca aggcacgtcg tttgtcagaa agtacaatga
    16561 gtaatatacg tcaaaatctc ttcttcgcat ttatttacaa catgctgggt gttccgttgg
    16621 cagctggtat tttgtatcca ctgacaggct tacttttaag tcctatcatt gcggcacttg
    16681 ctatggcgct ttcttccgtg tcagttattg ttaatgcgct taggttgcgg cggttaatat
    16741 tatgaaaaaa aaaactattg atgaaagaaa agaaaaaaaa gtagatctat actaagggga
    16801 gtagttggtt gtttgtaagg caacatcatg gttattccag tttttatgat actacttggc
    16861 atcggttttc tcattaatgg agcttatttg attaataagc tgcttcaaca aacaaatcat
    16921 tatcgccggc agtatttagt ttatcttgct tttatcggcg tttttatttt gagttattcg
    16981 gccagcattg tttatggtct tttggtggat cctcattgtc ttaattattt ttacgccact
    17041 gtgtttttac tgggtgccac ctatattgat tcatcgtgtc atctcatgac caagacgatg
    17101 caaaaaatgg aattgatgga tgactttaaa aatcgctatg aaacgttacg ccatcatgcg
    17161 acttatgatt cgctgactgg gtgtaataac cgggaatatc tttttgagat gttgaatacg
    17221 cgtttccaac aaatacaact ccatggcggt gaagtcgttg ttctctttat cgatttagat
    17281 cgatttaagg cagtgaatga tcgctttggt cacgatacgg gggatactgt tttacaaacc
    17341 tttggccaat tcctaagaca gcgtttacga aaagatgatg tggtagcacg ttatggcgga
    17401 gatgaattta ttgtattgat ggaaaatact tctttaagtg aggctaagaa gattgctgaa
    17461 aatctggtga ctgctgctac ccagtccctt aatggagaac tattggatac attggattta
    17521 ggctgttcta ttggcattac tcaaatgagc cgtcactcgg tctcagtgaa tgccgctatt
    17581 aaagaagcgg atttggcctg ttatgcagcc aagcaaagaa aaataaatgg tgctgtttgt
    17641 gtctacagtc caatgcttgc gacaacaagc aatcacatgg tttcttgtat caagcaaatg
    17701 accgaataga aaattaatat gcaccaagca atcatcttac aacagtagcc tgcttgtgcg
    17761 ataatgagcc actgttctat gctggatatc gtttcagtct ttattgttac ccgtcttgtt
    17821 taatcctatg ggatagtaga tactgaaata aagaataata aaaaaaggta ttgattcatg
    17881 actcactctt taatgaagag cgcactgcta ttcatcgttc ctgctgttct tatgggttgt
    17941 tcttacaatg ccaaactcac gccagaaggc gaaaaagtga tccttcttaa ggcagaaaaa
    18001 cgcttaaaaa catgcacttt tttaggggaa gtcagagcgt acgatcgcaa tggcatgagc
    18061 caatcctatc aatcccatga acatttgatt caggatgagc tgaatatcct aagaaacaaa
    18121 gccgcccttt taggggcaaa tactcttttc gttaacgaac atcagcaaac ttatgaaggc
    18181 aatcccaaaa atgatttggt tgcggaccat tggatggtgg ggagagccta ccaatgcaag
    18241 acaaagcaat agtcattttt aagtcttacg gttatggaaa acaatagggt ttggcttttt
    18301 tcttgttggg tggtgctttt gtggctgtat tcttctttag ctaatgcatt gactcttcaa
    18361 gaaataaccc aaattgcttt ggcgaataac aaggatttaa aagcagctcg ctataatgtg
    18421 gcgattgcaa aggcccgttt aatccaagcg ggcctttggc ctaatccaag tcttaatctc
    18481 tccaataacg acgatcgctt atttaatgat gaaggggaat attcacgcag tgcaggtttt
    18541 agtcaagcct tccccatttc tggacgtatt gcaaaacaaa aaaccgttgc acgccttgat
    18601 gtactgaaag ccatggccga aattggggaa gcagagcgcc aactcagtgc caaagtagcg
    18661 aatgcctttt atgcagcggt gattacggaa cgtcgcctgc aacaattgaa ttatttatta
    18721 aaaatcaata aagaattagt taatgtcatc cataaccgct ataatgcggc ggagatttcc
    18781 aagctggatg agaatgcagc acgcatagag tacctgcgta ttgcccaaga aaagcatcta
    18841 cagcatagtc ttcgcatcag tcaaaatgcc acccttaatc aattaatggg gcgacaagca
    18901 gatactcctt tatcacttaa gggcgattta gcaacaggac gacaactccc gaaattagcc
    18961 ctcctaaaaa catgggcgct taaccatcgt cctgaccgcc aagccattgt cttatcgatg
    19021 agtcgtgcca atgccgacca ccgcttggca aaagctgagc gttttgctga ttggacggta
    19081 gggcttggtg tccaacaaga taaaattgtg gtggaagagg ggtcacctca acctgccgac
    19141 cgcaccttag gtgtgacact ttcggtaccc cttcctcttt taaatcgtaa tcaggggcga
    19201 attttagaag ccagtcatac ggcaactcaa gcgatgatgg cgttaagcgc cttgaattta
    19261 agtattgaaa cggaaatagc gagtaattac ggacagctga aagcgcttaa aatgagtctt
    19321 agagaaaccg aaacggtttc tcttaagtta ggggttgaaa acataaaact ggcacaagat
    19381 tcttatgaaa acgggcaaat ttcgcttttg aacgtcttgc aagtgcaaaa gcaacaaaat
    19441 gatttgcaaa tgacctatct cgcaacgaga gaaaaatatt tacaagtgta tgtggctttg
    19501 tgtacagccc ttggccctgg gaatacccaa ggattttgtc cttacttagc ttatcaaagg
    19561 aatggtgata gtcaacatgt tacgacaacg aaatagaagg cttgccttca tcagtatttt
    19621 tttaagttta tttctttcta tgcaagggtg ttttgcccat ggcgaagaaa ttgaagtgag
    19681 tgaaggaggg gccaaaggcc cagtgcattt aacaccgcag caaaccaaaa tgttggggat
    19741 taaagtggca gaagccacca atcgtcctat ggagcaaatg ttgggtttga atggccaaat
    19801 tcaactttta cctgatgcgc aagcagatgt cagtattcgg attagtggca gtgtcaccgc
    19861 cattgatgtg aatttaggcg atatagtcaa ggcaggacag cgcttagcta cagtccaatc
    19921 aagattagtg ggcaatccac caccgagtgt tgcggtcaca gcgccgattg caggcctcat
    19981 tgatgccagg aatatcaatt taggtcaagc ggttgagccc aataccgttc tttttcacat
    20041 cagcaatcgt gataaattat tcgtgattgc tcaagtgtat gaagaagatt taggcaagat
    20101 taaggtcggg caagcagtga atattcatgc cctaagttac cccaggcaaa tctttaaagg
    20161 ccaaatgact ttaatcgaac cgaatcttga ctcgttaact cgtacagtga atgtcagaat
    20221 taccttagac aataaggagg ggcttctcaa accgggtatg tttgtacgtg ctaacgttat
    20281 tttagcttat aatgaggcag cactggctgt gcctagtgcg gctttgttgc aagcggataa
    20341 tgagaccttt gtttttgtgc aaaacgacga cacctacgat cgcgtcgtgg tgaaagtggg
    20401 tgcggtggat gatgagcatt cagaaattac cgatggctta gttcctggcg acctggtcgt
    20461 aactcaaggc aaccgtgagc tttatacctt atggttaagt gggggcagta aaccaaaagc
    20521 aggccaagag gagcctcatt aaatgtttac ttatctcatc gcctggtcct tacgaaatcg
    20581 tcccttaatt ttagctttga ccattatcct ctgcttgcta ggcggctata ccttgcagca
    20641 aatgccagtg gatgtgtttc ccgaatttgc cccgcctcaa gtggtcgtgc aaacgcaagt
    20701 gcctggcatg gctactcaag atgtggaaac cctggtgact tatctcttgg aaagtgccat
    20761 caatggaaca cctggggtgg ctagtgtccg ctcaaaaact tcggtgggat tatcgacgat
    20821 tacggtggta ttcgatgaca aaacggacat ttatcgcaat cgtcaattgg tgaacgagcg
    20881 tcttcagcaa gcagtcaatc gccttcctcc aggggctaat gcaccagtga tgctgcctgt
    20941 gacttcggcg gtgggatggc ttgttaaata cgcgcttatt agccgcaccg caagccctga
    21001 agcgttgcgt acgatttccg actggaccat tcgtcctcgc atcttggctt taggtggggt
    21061 ggctgcggtg gtttccgttg gtggagaagt caagcaatat caggtgctgc ttgactcaca
    21121 acgcatggtg gcttatggca tcaccattga agaggtacgc caagccttaa cgtccgcgaa
    21181 tcaaaacgtt cctggtgctt ttgtgcatca ggcgggtacg gagtttgtgg tgagtaccat
    21241 gggtcgcatc aaaacactgg atgacattaa aaaaacattg attgtggttc gtaatggagt
    21301 tcctattacc attaacaatg tagcaactgt cgctttcggt ggtgaaatca aacggggcga
    21361 tgcgtcttat aatgcaaaaa atgcagtcat tggcacgatt tccaaaatct atggagcgga
    21421 taccttaacc accacctata aggtcgaaca agcattagag gagatcaaac gctctttgcc
    21481 agcaggggtg gaattagtaa ctgaagtatt tcgccaagcc aattttattg aatcggcgat
    21541 tcacaacttg actagagcct tgattgaagg agccatcatc gtcattgcgg tgttgttttt
    21601 gtttttaatg aattggcggg cttcctttat tacgtttctt tccatgccgg tttctttcat
    21661 agtgggcctc ttggttcttc actattttgg ttttggtatt aattcgatga ctttaggagg
    21721 gatggcgatt gccattggtg aagtggtgga cgatggtatt atcaccgttg aaaacgtggt
    21781 gcatcgccta cgattaaatc gcttggccaa gaagtccttg cccgccattg aaattgtttt
    21841 tgatgcggtt ttggaaattc gaagctcggt cgtttatgcg accattatca ttagcctcgt
    21901 atttttgccc attttctttt tatcaggaat ttctgagcga atttttagtc ctttggcgat
    21961 tgcttacatt gcgtcagtga tgggctctct tgtggtttct attaccacgg tgcctgctct
    22021 ttgttatctt cttttagtgg gaaaaacgga aaaaaaacaa gatactcaag tcgtgatgca
    22081 tgctttgcct caagaggcgc gttatcattt gaccgaagaa gaaatcgccc aacatgctca
    22141 agcggaaact cgttttgtga tgtggttaaa aaaacatttt ttaacaggtt taaattggtc
    22201 gttggcgcat tgtaaaacgg ttgtaactat ggctataggc gcctttcttt tcgctttagc
    22261 cttacttcct ttttttggaa cttcgttttt gcccgaattt catgaaggca attttattat
    22321 cgcgatgagc acccttccag ggacttccct ggatgaaacc atgcgcttag gacaacaagt
    22381 gagcaaaact ttgttgaagt atccgcaagt cgtttccatt gcccaacggg cagggcgtag
    22441 tgagttggat gaagacgcca tgccacccaa tgccagtgaa tttgatgtga gacttgattt
    22501 tgacaaagac aaaaccatgt caccggatga attgttgcga cgtattcgag cagatttggc
    22561 gaacattccg ggtgctgtgt ttaatgtcgg gcaatttatt gcccatcgta tggatgaagt
    22621 gctctcagga gtcagagcgc aagtggcggt gaagctattt ggcgataatc tggccaccct
    22681 taacgaatta ggtcaatcgg ttgaagccat tttaaaaacc gttccagggg tggtggatgt
    22741 gaacaaagag caacaaatca aggtggcgca gttggtcatt accattgacc gagagaaagc
    22801 ggcccgttat ggggttaatg tggggcagat ttcagaagat gtgcaagttc ttcttaatgg
    22861 ggtgacggta tcgagtgttc tcgaaggcca acgaacgttt gatttgtatg tacgcatggg
    22921 tgaagaaggg cgagacagca tgaaagccat tcaaaacatg ctgattgatg cgcatggttt
    22981 ggcgaatcag gaaaccaaaa ttcctttgcg tgcggtggcg gatattcagt tagagcctca
    23041 accttttgca attaatcggg aaaacgtaca gcgcgtcttg gtgattgcgt ttaatgtgca
    23101 agggcgggat ttaggcagtg tgattcaaga agttcagcaa cggattaagg aaaaagtgtc
    23161 gttgccaagt ggctatttca ttcaatatgg cggacagttt gaaagtcaac aacaggcctc
    23221 tcgcgtgata attctgtttg gcgcgttggc cattttcatc atgttgattc ttcttcataa
    23281 agcgtttggc accttccgtg aagcattatt ggtcatgttt aatttgccgc tcgctttgat
    23341 tggtggcgtg gtttctttgt ttctagcaag tggggatatg agtgtggccg ccatgattgg
    23401 gtttatcact ttatttggca ttgcggcacg taatgggatt attttagtaa gccattacaa
    23461 tcagttgcgt acccaaggac aaccacggga acaggtagtc attcaaggca ctcttgaccg
    23521 tttggtgcct gtgctcatga ccgcagcaac ggcagcgctt ggcttaattc ctctgttgtg
    23581 gggctcgcct tctgggaaag aattggagcg tcctttggca caagtattgc ttggaggatt
    23641 gtttacctcc acgtttttaa atatgtttgt ggtgcccaca gtgtataaca cgatggaaac
    23701 ttggcgtgaa aagagacagt atgcaaaggc aaactctcaa ggagaatcat aatgaatcaa
    23761 ataacgaagt cttttaataa aaaagcacta ggtttgttta acctgcttgt tgttttgctt
    23821 tttttattgt cacctgcgta tgctgctgct aatcatgaag aggaagctca gtctatccca
    23881 actaccattc ctgccatttg ggaggccatt aaccaacata ctgcagccat taataaagcg
    23941 ttggctgaca atcagctgga taccattcac caacatgcat ttgcgattcg agacttagtc
    24001 aaagcactgc ccgatttaag caagaatttg tctgaagaac aactcaaaaa tctgaagcaa
    24061 aacgtggggt ataccgagca actggcaaag cgccttgata aaacgggtga tgccaatgat
    24121 aaagaaggta cgcaagccaa ctggcaaaaa ttgcaaaaag tgctggcaca aattcgagcc
    24181 aattactctc tttcaggatc ctagactact tcttgattca cagccaatca acttgcctga
    24241 tggtaagctc tgcctttctg ttttggaata aaacataaca ccacacaaca aaaaggccat
    24301 ctgattcatt aataataatc acagaaggca attgatttaa tgcccactat gatgcccatc
    24361 ccaccaaatc caatgtcctt tccactttat ccatccagga tgtcctccgt gaggagcttt
    24421 gtgatgtccg ccatgatgat agttatttgc ggctaaaacg acgttacctt gagggtttac
    24481 tggagcatta ggaataaacg caaaaacaac aggagcttta tgtaagtata agtcaggatg
    24541 tagacttgct cagttattga gcagtacttc gagttgtaca ctattccgag ttacctcaaa
    24601 agatttgact gaatacacca aagagacatt aagaaaaggg atctcgttct gatgcgtatc
    24661 tattttgata aatagagtca ttgattttta aatgacccta ttcatgccaa tttttatatc
    24721 ttaatttaat gggggaataa acactttaat tttataagga tttgctatga atattgtaac
    24781 agtattaagg aacaccattc ttttaatagc acttttttat tcttttcttt agtgtctcag
    24841 catgcacgtc tttgactcca acaactaaaa aaactcaaaa taatggcaaa aatcagagca
    24901 gtggtctaaa gcataaaaat ccttctattg ggcgtgtttg ggccagtcat actcgaaggc
    24961 attagtacca ccttcgtatc cagtaaacaa taacgagagt tcctagtttt ttacattcct
    25021 atttaagtaa gtaaaattaa ggcaataaaa gcgaaattgg tggtaagtca tcgagattac
    25081 agtcacgtga ttcactctcc cctaagattc tgcaagtgct gttgagccgc tgggtacccc
    25141 tgctcagcag ctttttgaag ccactttagt gcttcatcat gattttgagg aaccccctgg
    25201 ccatcatgat aaattgcagc aagcgcgtat tgagcttgag gtaacccttg attggcagca
    25261 agatgaaacc atttcattgc ttcaatatta ttttgtttca tacctttccc tacaagaaaa
    25321 ccgatagcga tattatactg agcgatagga tctccttgct tagcagccag ataaaaccat
    25381 ctcattgcct caaaatcatt ttgctgagta tgattacctt ctacatacat tgcactgaga
    25441 gtacgctgag acgcaagatc gccatgttca cctgctttac gaaaccatat tattgcctca
    25501 ccatcatttt gttttacacc cttgccagtt aaatataaga tacccagagt acgttccgca
    25561 tctggaaatc cttgttcagc tgctttgtgg atccatttaa cagcttcccc atcattatgt
    25621 gaaatgccaa tgccttctat gtagagtaca ccaagattaa attcagcgac gggatcattt
    25681 ttttcagctg cttttcgaaa ccatttcatg gccttgctat gattttgagc aacacctgtg
    25741 ccggttgtgt acgttaaacc aaggttgcgt tgagccatgg tatcaccctg ctctgctgcc
    25801 ttttgaaacc actttactgc ctcagagtaa ttttggggta cctttttgcc aatcatataa
    25861 tctatcccgc ggttattcca atctgcggga tttttgtgtt gaacaacata attgtccaca
    25921 tattctttaa tcgtattaat gattccagaa taaatgctca caccaacgat aattccggct
    25981 aaagggaatg ctaacagacg taatcgcata caatactgtt ccttgttaac agcgacaaat
    26041 ggaaacctat cttattattg aatcaataaa aattcaaaac aaaatactta attttattgg
    26101 ggattccctg caaataaatt gctaaggctc aaggaactag aaacactccc tggccaaaga
    26161 aatccacaag aggtgatcat ttagtcgttt aactcatgaa aagatacccc ctagacagaa
    26221 gggatgggtt agattttgtg ctatcattta ttatatctta ttgattatag tgctaaatgc
    26281 caaggatgtc ggccatgaat ggactagaaa aacaatgttg tcccaaggaa gttttattga
    26341 ctcagcggga agaattgtca gttggtacgg attcatgttg tgttcctact gatgcaagca
    26401 cctcggataa tttttttccg ttctttaatc agctaattca agccaacctg gcaaaatgga
    26461 caatgggaat aagtcctgcc gcaataggtt cgtcctattc cacttggctt tggcaattaa
    26521 tccaatcccc aggaaattta tgggaactgg ctttttaccc tatttttcat gccaatgatt
    26581 gtattaataa tattgtttgt gttgaccgtg ctgctggggg taaagacgtg cggtttaaaa
    26641 aagacagttg gcaacccatg ccatggcgtt tgttcgcaga aggatttttg caggtggagg
    26701 attggtggcg acgtgctaca acgaatgtac cagggttacc tcgtccggtg gagcgcacgg
    26761 tttcattttg ggcacgccaa tggcttgatg ccctatcccc ttccaatttt gtctggtcga
    26821 accctgattt attcaacgaa gccatacgca cgggtgggct taaccttatt caaggcagcc
    26881 aaattgcatt ggatgactgg ctagaaaaac taaccggcac accaccaaca ggctccgaac
    26941 actttatccc aggccaacag gtggccatta cgccaggacg agtcgtgttt cagaatcact
    27001 taattgaact cattcaatat gagtcgcaaa ccaagacagt atacaaagag ccgatactga
    27061 tactgccggc ttggattatg aaatattata tactggattt gtccccacat aactctttag
    27121 tgaagtggct cgtcaagcag ggacacacgg tatttataat ctcctggcgc aaccccgata
    27181 aagaggaccg tgatttgggg atggatgatt attatcgtct tggtgctatg gcggcgattg
    27241 atgcggtctc caccattcta ccagaggcca aaatcaatct gatgggctat tgtttaggcg
    27301 gcaccttggc tctaattacg gcgtccgtga tgggaagaga caaggatgaa cgtttaaata
    27361 gccttacgct gttggcagcc caaggggatt ttaccgaggc tggcgaatta atgctctttg
    27421 tgacggaaag ccaagtggac tttctgaaaa gcatgatgcg ggaacaaggg tatctggaca
    27481 cgaagcaaat ggcgggttct ttccagatgt tgcgtgctta tgacttaatc tggtccaaga
    27541 tgattcaaga ttacatgcat ggcatgcggc gtggaatgat tgatttaacc gcttggaatg
    27601 ccgatgccac tcgaatgcct tataaaatgc acagcgaata cttagaaaaa ctctttttaa
    27661 agaatgattt tgctgaaggt cgttatacgg tggaaggaaa accggtggcc gccgaaaata
    27721 ttcagctgcc tgtttttgct gtgagcactg aaaaagacca cgtagctccc tggcagtcag
    27781 tgtataaaat ccacttgatg acggatagtg atgtgacctt cgtcctcacg ggtggcggcc
    27841 ataatgcggg tattgtgagt gaaccgggac atccaggacg ttcttaccgt attcatgaac
    27901 ataaagaggg cgaggcttac ttaaaccccg caaattggct aaccgttgca gaaaagcgag
    27961 agggttcctg gtggcgcggt tggcatgagt ggctggtgct gcaatccacc aagaaacgca
    28021 ttcctccacc ggttatagat acgtcactac cagcagcccc cggtacttac gtgcatcaga
    28081 aatagaaggg aagaaggatg gagcaggaat ttcttgagaa tgtgaccttt aatgaacttg
    28141 aagtgggccg tcaggctagc ttaagtaaaa cactcactca agacgacatc aatttgtttg
    28201 ctgccatgtc gggcgatgtg aatcccgctc atgtggattc cgtgtttgcc aagagtacta
    28261 tttttcatgg gattgttggg catggtatgt ggtctggctc attgatttcc gcacttcttg
    28321 gcaccgtttt acctgggcca ggtacgattt atcttgaaca ggatatccaa tttaaaaagc
    28381 cagtgcgttt aggcgatacc atcaccatta ccctcatggt taaggataag cacaccaata
    28441 aacccattgt gatttttggt tgcaagggta tcaatcagcg tggagaaacc gtgattgagg
    28501 gattagcaac cgtccttgca ccaacagaaa aaatgcgagt ggcgcgtgcc caccttccgc
    28561 ctattgaaat ttataaccac gatcgctttg aagccattat taagacgtgt cgctgtatgg
    28621 ctgctgtccg tacggcaatc gtgcatcctg taacgagcaa cgtcatggaa gcggtgaacg
    28681 atgcgatgaa ggcaggactc attactccta tattggtggg acctttggct aaaatgaggg
    28741 ctgcagctcg tgatgcccat attgatttat cccaatggga aacgatggat accgaacata
    28801 gcgatgctgc cgccaataaa gctgctgatt tggccgctgc aggcgagatt gatgccatca
    28861 tgaaaggctc attaagcacc catgaattga tggcagcgat tgtgctttcg acatccggtt
    28921 tacgcaccca acaccgagtc agccacgcct atgtcatgga tgtaccttct taccataagc
    28981 cactgattgt taccgatgca gcgattaata tcgccccaag tgcgagtgac aaagccgaca
    29041 tttgtcagaa tgccattcac ctttggcgag tattgtatgg tgagaacaag aagcccaaag
    29101 tagcactatt ggcagctatc gaaaccgtca atcccaagat gcaagccaca gttgatgcgt
    29161 cgattttatg taaaatggct gaccgaggtc agataaccca tggtatcctt gatggtccct
    29221 tggcctttga taacgcaatt aacaaagagg cagccaaaga aaaaggaatt gtttcactgg
    29281 ttgctgggga tgctgatatt cttttggtgc ctgaaatcga atccggtaat attttagcca
    29341 agcaactcac ctttttaggg catgcggatg ctgctggcat tgttttaggg gctcgtgtgc
    29401 ccatcatttt ggtaagtcgt gctgattcac tacgcacccg gcttttttcc tgtgctctgg
    29461 cggttaaaat ggccgcagcg cgaaaggaag gacgaattaa atgacgaaca tcaaagcctc
    29521 gtgccttctt gtgattaatg tgggctcctc cagtatcaaa tttcagcttt tttcaagaca
    29581 acccaatcca aagattttag cttatggaaa agtagtcgat attgggtgta agccttcatt
    29641 tacagtcacc gatgacctca aaattctaga agagagcgag aatgaaaaag cacgatttgc
    29701 attaaagtat ttctgcctca aaatggccca attcatgggt atgatggcaa tggcactagg
    29761 tgaagttgat gcgattgttt ttaccggtgg tacaggtgag aattcagcgt ttgtgcgtga
    29821 tggtatttta cgtcaattgg agtttctgaa actcgtattg ttgctgccaa tgaagaacgg
    29881 atgatggcag tgcatgccat gtcacttctt gagcaccaaa caggagttgg aagcttatga
    29941 agaataaaaa aggactgatt attggtattg ctaatgaaca ctcaattgcc tggggttgtg
    30001 ccaaagtatt gtatgaggcc aattgcgaat tagccatcac gtatcagaat gagaaagcca
    30061 agagttatgt tcagtctcta gcagaacagg tttcagcatc cattttcatg ccccttgatg
    30121 tgacggataa agcccaattc gatgcattat tccaaaggat aaaggagagc tggagtcatc
    30181 ttgattttgt cattcatgcc attgcttttg cgccaaaagc ggatttacaa ggtcgggttg
    30241 tcgattgctc caaagaggga tttatggtgg ccatggatgt ttcttgtcat tctcttatcc
    30301 gattggcaaa agcggctgag ccactcatgg tcaatggagg aagtatcatg accatgagtt
    30361 attatggtgc agaaaaagtc gttaaaaatt acaatctcat ggggccagtt aaggccgctt
    30421 tagaaacttc agttcgttat cttgctatgg aacttgggag taaaaagatt agggtcaatg
    30481 ccatttctcc ggggccaatc agtacgcgcg cggcatcagg gttggccgat tttgataaac
    30541 tgatggagaa ggcagccaat gaggcacccc ttcatcagct ggtgaccata gaggctatag
    30601 gtgagatggc ggcgtttttg gtgtctgata aagccgtttc tattacgggg caaattctct
    30661 atgtggatgc cggttataat atcaaaggct aacaagcttc ttgatgatta catgtattgc
    30721 cctatttttg cataacacac tacttaataa aatccgtttt taattgaaag catctggttt
    30781 tttacattga attggtatcg gttttgtttt atggatttca agaattaggt atttatagag
    30841 aaagaagcat ttactcataa ttaaaggcat caaaacagtg gtttaagtat ggattaaatc
    30901 cagttagaat gtcaaatcat ggtgtgaaca gattatcttg gttatcttaa ttcaaggaaa
    30961 aaggaatggg gcttttttta aggttggttt cactggtttt actgagcttg tcattcagta
    31021 caaaagccaa tacaaccttc acattcaaac aagccttggc tatggcttat cgcaataacc
    31081 ctgaattgca agctgaaata gataaagcgc aagccatgag aggtgctttt attcaaagcg
    31141 gactttatcc taacccccaa cttaatttaa cagcagaaaa ttttggtggt tctggtgtct
    31201 attccagtta tgaatcagca gaaaccacgg cttctgtgac acaacctatt cctttagggc
    31261 atcgtcttca gtatttgcaa aaagcaacct atgcggatta tttgacttct cttgcgagta
    31321 ttaaagtgca aaaaacggtg ctttatatgg cagttggtaa cgcgtacgtg gatgctttat
    31381 atgcagaaca atggcataag gtaaccaaaa aattaaccaa acttaatcaa gacattgtag
    31441 tcgccattga acgccgggtt aaagcgggtg ctggtgcgga gttggatttg cgattagccc
    31501 aagtgcgctt gggtgaggct cgaattcagg aaagcaaagc atcacgtgat gctttgctac
    31561 aacgagcaag gctcgcccgt ctattaggtt atgggttaag aattgataag cctttggtgg
    31621 ataaaggatt gcctggtctt actctggatt ggtccgaatt aataaaaaaa ctaccgcaaa
    31681 gcccgcagct tgtgcaaatg caattgcaat tacaagccag gcgagccacg attactgccg
    31741 ttaaaaaatc cgtttggcct gatttaaata ttcaattggg tggtcgccat ttttctgatg
    31801 atggcagtaa tgctgcggtg atgtctgctt ttgcagaagt acctgtttat aatcgcaatc
    31861 aagggaaaat catgacggct gaagcgcaat atactcaagc ggctcatgag tttcaaagca
    31921 cgcgtcttga ggtgcgtcaa aatgtctatg cggttttttt acaagcccaa caaagtcaat
    31981 acgaagccaa tttagtcacg gattccttat tgccctccgc gcgtaagtcc atccaattgg
    32041 ctcaagaggg ttatcaaatg ggtcgttata cttatgtgga actttatacg gcattaagta
    32101 ccttgtatga ggaagagcgt cattatcagc aggctcatgc cgattatcat aaatctttaa
    32161 ttcagatgac agggctttta ggattagagc ccattaagga gagtcaatga aatttcaaag
    32221 gccattcatt ccttattttc tatcgctgat tcttttattg agctttagtt cgttttcctt
    32281 aagagctgaa aacaaccact ctgaagaaag cgaacatcaa aagactatgg aaaaaggtcc
    32341 acaaggtgga cgtttgttta aagagggaaa tacggcattg gaacttttaa tttttgaaag
    32401 gggtatgcct cctcgttttc gtgcttactt gtatcaaaac ggaaaaatga tctctcctga
    32461 taaagcccat ttaacagttg aactcactcg gtttaatgat aaaaaagagg ttattacctt
    32521 cattccagtt gagaattttt tacaaagcaa ccaggtgatt cgagaacctc attcgtttga
    32581 tgttaccatt caattaacct tgcaggaaaa gcgttacaat tggcattatg ccagctacga
    32641 ggggcgagtt aaaatagtag ctgaggtttt aaaggcggct gatattcaaa tggccaccgc
    32701 tcaaagtcaa accattaaga ctcaattaaa agtggtaggc aagattgctc ctaatcgcga
    32761 tacgttggct ccaatttatc cgcgctattc aggtattatt cagtccatga ctaaaaattt
    32821 aggtgatgaa gtgatgaaag gagaagtgtt agtcactatt gaaagtaatg aaagcttaca
    32881 aaactatacc attactgcgc ctattactgg gactatcgta caaaaatatg cgaccaatgg
    32941 agagcttgct caaaacacca agcccattta tgaagtggct aatttggcta cggtgtgggc
    33001 ggattttaca ttgtatcgca aggaagcgcc ccttgtgaaa caaggtatgg aagtcactgt
    33061 gacaggggat gagggcaaac caaaatccat cagtactatt tcttatattt cacccttggg
    33121 tgttgaggat agccaaacca ctttggcgcg tgccgtactt tctaatgaca ggcgtctttg
    33181 gttgccaggg atgtatgtga atggggctat tactattaaa gaaaaaacag tgccggttgc
    33241 tgttcttctt tctgccatac aacgaatgga tggaaaagaa gtagtgtttg tacagcaagg
    33301 ggattatttt gaggcaacac ccgttatttt gggtgaaaaa gacagtcaat gggcagaagt
    33361 ggtgtctggg cttgatgtag aacaacgtta tgtcagtaaa aacagttttt ttatgaaagc
    33421 agagcttgga aaagaaggtg caagccatga gcactaagga ttaaagatgc ttgaaaaaat
    33481 cattcgcttt tccctgaaac atcgctggtt tgtgttgtta ttcacgcttg tcattgccat
    33541 tcttggtgtg tataacttcc agcgccttcc tattgacgcg gttcctgata ttaccaacgt
    33601 gcaggtgcaa attaataccc aagcctccgg ttactcccct tttgaggtgg agcagcgcat
    33661 cacgtttcct attgaactgg ctatgagtgg gttacctagt ctggattata ctcgttcctt
    33721 atcgcgttac gggttatcgc aagtcaccgt ggtgtttaag gacggtacca atatttattt
    33781 tgccaggcaa ttgattaatg agcgtttaca agaggtcaag gacaaattgc ctcctggggt
    33841 ggaaaccacc ttaggaccca tttcaactgg tcttggtgag atttttatgt acacagtcac
    33901 caacaaaccg aatgtaccca taagccagca ttataatcct acggagttgc gcaccattca
    33961 ggactggatt attaaaccac aacttcgcaa tgtggagggg gttgctgaag tcaacaccat
    34021 agggggttat gagaaacaat tccatattac tccagatcca tccaagttgg ttcgctaccg
    34081 tttaagttta aacgatgtgg tggaagcctt agagcgcaat aacgccaatg tcggtgcggg
    34141 ttatattgaa accaatggcg agcagaattt aatccgtgtg ccaggacaag tccaaaatat
    34201 ggctgatatt gaaaacattg tcattgctag ttttgagggc acgccagttc gtattcgtga
    34261 tgtcgctgaa gtggctttag gcaaggagtt acgaactggg gctgcgactg aaaacagtaa
    34321 agaagtagtt ttaggtacgg tttttatgtt aatgggagaa aacagtcgta cggtttctga
    34381 gcgcgtggcg gccaaaatga aggacattaa caagactctt cctgaaggcg ttgaagcgat
    34441 cactgtatat aaccgaacaa cgctcgtgaa tgcgacgatt aataccgtga agaataattt
    34501 gttggaaggt gccttattag tttgtgtgat tttatttttg tttttaggaa atatccgtgc
    34561 cgcactcatt accgcgatgg ttattccttt atccatgttg ctgaccatta ctggcatggt
    34621 ggagaatcaa atcagtgcca atttaatgag tttaggggca cttgattttg gtttgattgt
    34681 ggatggggcg gtcattattg ttgaaaactg catcaaacac ttggctgaac agcagcatgc
    34741 gctccatcga gttttaaacc ttgaagagcg tttaaaggtg atttcgtatg cgactacgga
    34801 agtcatcagg cccagtatct ttggggtgtt tattattacc gtcgtttatt tgccgatttt
    34861 gacgttaact ggtgtggaag gcaaaatgtt tttgcccatg gctcaaacgg tcattatcgc
    34921 acttttagcc tccatgttgt ttgctttaac ctttgtgcct gcagcggttg cgattttttt
    34981 acgaggacat cttcaggaaa aagaaaactg gttggtgcat tatctgtctt tagggtatgc
    35041 caaagtgcta cgacgttgtt ttcatgcgcg acgagtagtt attagtgctg ccgtggcatt
    35101 ggttgttgtt agcttaggga ttgcctttca tttaggcgga gaatttatcc caagccttga
    35161 tgaaggggac attgccatgc acgccatgcg cattccaggc accagtttga cgcaagcgat
    35221 tactatgcag gatttagtgg agaaacgcat ccggcaattt tctgaggtca agaatgtttt
    35281 tgcaaagctg ggaactgccg aagtggcaac cgatcccatg ccgcctaatg tggctgacac
    35341 ctttatcata ttaaaatcaa ggaagaagtg gactaacccg aaaaaaacca aaccagggtt
    35401 agtgcaggag attgagagtg ctgtccaaca aattccaggg aataactatg agtttaccca
    35461 gcccattcaa atgcgcttta atgaattaat ttccggggtt cgtagtgatg tagcggtcaa
    35521 ggtgtttggt gatgacatgg atactttgct gaaaacagca gaagcgatta gtgcgcaact
    35581 aaaacaggta cctggcgctg ccgatgtgaa ggtcgagcaa gtcagtggtt tacctctctt
    35641 gacggtagaa attaatcgtg atgtgctggc ccgttatggc ttgcaaattg gtaccgttca
    35701 agaggcggta gtgatagcca caggcgggaa aaaaggtggt gagctttttg aaggagataa
    35761 gcgctttgat atcgtggtac gtctaccaga atccttacgt tccgatccga atgtattgcg
    35821 gcaaattttt attcctttgc ctctgtctaa agatggtgag cagcatttta tcccgttaag
    35881 tgaagtggct tctcttattc gcagcgaaag ccccaatcaa atcagtcgtg aaaatggaaa
    35941 gcgtcgtgtg gtggttactg cgaatgtcag aaaccgtgat ttaagttcgt ttgtgagtga
    36001 agcaaaaaag cgcattgatg gccaggtcaa actaccgagc ggttattgga ttacctgggg
    36061 aggtcagttt gagcaattgc aatcagcata ccaacgattg caaatcgttg tacccattac
    36121 cttgttaggt attttcctcc ttctgtttat aagttttggc aaggtcagag atgctctgtt
    36181 ggtatttaca ggcattcctc tggctttaac gggtggtgtt tttgctttgt ggttaagggg
    36241 aattccttta tctatttcgg caggagttgg attcattgcc ttgtcagggg tggccgtact
    36301 caatggtctt gtgatgatta cttttattaa taaattacgc gagcagaaaa aagtatattt
    36361 gaaagatgct gtattgcaag gttcactggc aagacttcgt cctgtactaa tgaccgcttt
    36421 agtagcctct ttaggttttg taccgatggc cttagctaca ggaacgggct ctgaggtgca
    36481 acggccttta gctaccgtgg taattggtgg tattatttcg tccacttttc tgactttatt
    36541 agtattgcct ggtttgtact atgtgtttca tgggcgtcga aaaaaaggcc aatctaaaag
    36601 cgaaccacaa gaacaaataa tgtaacgagt ccttttggca tgccaactct gtaaagaaag
    36661 aactctatct tagttctggc aagtaagtca aaggaaggat agcgattaag gctatcaatc
    36721 caaaaaaggg caggagaaaa cctgaggtgg cgatggctct ttttaatttc gagtgttgat
    36781 ttttagttgc aatcatcatt agcattccta gaagaaacgc caaaactgca atcgtaagag
    36841 gcatgtgaga gaaaaggaaa ttcatcaatg ctccattaag tggcttcaca tacttagttt
    36901 aaatgatatt tctttgaaag tgcaaactag cctggagttc catttgacaa aataggtcgg
    36961 tgcactatat ttataattaa acacttgttt aaccgtttaa ttaactatgt taacagaaga
    37021 ccaaattatc acattaagtg acatcttaca tttaatgggc gaagccaatc ggttaaagct
    37081 cctcttgagg tgtctggaag gtcccaaatc cgtatctgag cttgctgagc aattacagct
    37141 ttctgtgcct ttaaccagcc atcatctgag tctgctccgc tcagcacgtc ttttaatagc
    37201 cactcgagaa ggcaaacaca tttactacag tatttatgac aggcatgtgc gctgcatctt
    37261 agaggacatg ctcaaacact ttactgagga aatggagaat taacatgggc tttttaaaaa
    37321 gaatttttgg caatggcttg agcgatcatc atggcggtta ccgtaactct catcatggtc
    37381 aaagaaatga ttatcgagag gactcagcta atctttcacg aggcaaaatg aattgcccac
    37441 gatgtcaaac ttctcttact cctgggatgc ggttttgcag tgaatgtggc agtagcctgg
    37501 aaagtgcact atgcatttgt ggtgcagtga ttgccatagg tgcgaaattt tgtggccaat
    37561 gtgggaaatc tttatgacaa cgcctttaag gttaacaacg ttttttgtgg caggactgga
    37621 ttgtcctgca gaagagcaac tgattcgacg acaattacag ggcattcctg aaattgaaca
    37681 tttggatttt aatttcattg ctgaagaagt gactattcac catcgtcttt cttccataga
    37741 tttgttgcaa caacgtattg cagcattagg aatgagtgtg cgttctaaaa gcggcatcca
    37801 tccagcaaaa gaaggaggga ggtctctttc tgattcgtct tggctactca ttattttagc
    37861 tggtgttttc gctctttttt ccgaattggc ggcttatttt ttaggcacag agcaatccac
    37921 ctggagtatt atccctgcct tattggctat ggtcttaagt ggttcaccta ctttcaaaaa
    37981 aggttggctt gctttgcgca ccaaagccat gaatattaac agtttgatgt tgattgcaat
    38041 cagtggtgct gtattgatag gcgagtggcc tgaggctgcg atggttaccg tgttgtttgc
    38101 tttggctgag cgtatcgagc gttattcgtt agataaagcg cgattagcta ttcgaagttt
    38161 gatgcaaatt gctccggaag tggctcgagt taaaatggac aatggtcaat ggcaaaccat
    38221 gccggtagaa gaggtaccac tagaggctgt tttcagggta aggccgggtg agcgaattcc
    38281 cttggatgga gtagtgattt cggggcaaag taccgtcaat caagcgccga ttacaggtga
    38341 gtccatgcca gtagtcaaac aagtagggga tgaggttttt gcaggtagtt taaatgagca
    38401 tggtgctttt gaggtccagg tcacaaaagc atcaggagat acgctgcttg ctaaaattgg
    38461 taaggccatt gaacaagcgc aggctgaacg ggcgccaact caacgttttg tggaccaatt
    38521 tgccaagtat tacacaccca tcatggtctt gatagcagtt cttattgcac tgttccctcc
    38581 attggcattg ggttatcctt tttatgactg gctctataag gcgttaactt tgctggtgat
    38641 tgcttgccct tgtgctttag tcatttcaac gccggtgacc gttgtgagtg gattggctgc
    38701 tgctgcaaaa catggacttc ttattaaggg agggagctat ttagagacag gttatcgatt
    38761 aaaattaatc gcattggata aaacaggtac tttaacagaa ggcaaaccaa ttgtgactga
    38821 ttttattgtt tatgataaga gccaaaccaa agagcaactg ttattgttgg ctgcaagcct
    38881 ggatagtcac tccgaacatc ctgtggctca tgctttagtg acgtattggc aacaagaaca
    38941 atccaatagt tccttgctgg atgtggagga attctttgct cttcctgggc gaggtgtgaa
    39001 aggactggtt cagcaagaac tttattacat aggcaatcat caattggctg aagacaatca
    39061 agtctgtaat tctgcgatag aacaagcact caaacgatta gaagaggaag gaaaaaccac
    39121 cgttatctta agcaacagct ccactgtatt ggctgttttt gcagtcgccg acacgcttcg
    39181 cacaaccagc caatgggcga ttgcaaggct tcaccaacaa gggataaaaa cagccatgtt
    39241 aacaggagat aatgcggtga ctgctcatgc gattgccaag caagtgggta ttgatgaagt
    39301 gaaagcgaat gttttgccta cggataaact ccaggccatt gattttctca tagagcaata
    39361 taaagcggta ggaatggttg gtgatggaat taatgatgcg cctgctttgg ccaaagcaac
    39421 tgttagtttt gcaatgggca aaggaacaga taccgcattg gaaacagcgg atgtggcctt
    39481 gatgaatgac aatttggcga tgcttccttt gtacattgat ttaagtcgca acacagcacg
    39541 gattcttcgt caaaacatta gcttatccat tgcaatcaaa gggatttttt tctttttggc
    39601 acttgcgggc atcgcaactc tttggatggc agtgtttgca gacatgggag cgagtttgat
    39661 tgtggttgtg aatggattgc gattattgaa ttcgcatcaa ggacattctg aaattcagga
    39721 aatatagcct agtgataaga agttaagtac aagaaggaca catagctatt gtttgtattc
    39781 atttggtgag ataacaatca atgattcaat atgttatgat ggattgagca gctttttatt
    39841 tggaaacagg agaagtaatg aatactcgag ggcttaagat tttctttttg atcggaatgt
    39901 tttgtttagg atatggattg actgcatgca ctaagacaac gacttctaaa gaagtgcatc
    39961 ctgagagtgg gtatgaaggg ggctttggtg gcgatgggga gcataggcat ccaaatcaat
    40021 aatcaataac gcaacagggt aagcatggaa atgaaggcat ggattatagg tgctctgtgg
    40081 gtgttgatta ttgtttcatg atttattttc gaaaaaagaa gaaaacgaca cgtttttaca
    40141 tgattttaat tatttccctt gctatattgg ctattttttt acatggcgcg tcatttcaat
    40201 tacattaatt tgatattctt atatgggata tgtagtggtc attgatttga gtacaagtag
    40261 taagtatggc gcattacttt tgatgtcttc ttgcccctgt atcgactatt cttcgcatat
    40321 tgccaaagtc aatgataatt ctttattcag gttacagtaa taactcaatc atgtttttat
    40381 actggaataa ccattagatc tttagtgtac gtttttgtcc cctgggcttc aaactccctc
    40441 ttaaaggtct ataatgtttt agaaattgaa gatgagtgaa tcaaaacaac agaatgataa
    40501 aaatcaaagg aaagaccatg gccattaggt atcttaattt taattcgcgt atcaacaaat
    40561 tattatccaa agtaatacta ctttttttaa tctcaatatc gcataccatg gcaagtgaac
    40621 aacctgcatc aaaggtgact attcctgcga cagtaccaga actattacaa gagatggatg
    40681 agtatagtct tgccatttac aaagatcttg aaatgaatcg gttaagtgag gtgcgtcaac
    40741 atgcctttgt tattcttgat ttaatcaatg ccttaccggg attaagtcag gatttatccc
    40801 caaaacaatt aaaaaacata caaagtcaca tcgatgctat cagtcacttg atccccgacc
    40861 tgattaagag cagcaatgct aacgataaaa aaactaccca acgccatttg gacaaattgc
    40921 aaaaactttt gaatcagctt catacttatt atttgaaaca aaaaacggag cattttaatt
    40981 agttttcaat ttattgactc ggtgagtttt tttgatcaag aattttgggt gcttgcaatc
    41041 ttttttctta ttgagcttag caaggcatga aatagggagg ttataattta aaatagggtt
    41101 ttatccaaac aatgaggatt tgatgaacaa ttctttccgc tttcttgttg tagaggcctc
    41161 atttgctgct cgaattgtta ttcgaagtca attaatgcag cttgggcaac tggttgatct
    41221 ggctactacc gcagaggaga tgttcgataa aatagcagtt aaaacgtatg atactctttt
    41281 aattgataat cacttgtaca actcactggg tgattatgat ttgattgcgt ttataaaaaa
    41341 caacaacccc atagtattgc ttctattatt gttatcactt cccaacaacc gcctattggg
    41401 aataaagtca ataccacact aaaaaattta tgtcattttt ataaaccttt tagtgagggc
    41461 aatgcgctaa aaattatcaa ttatgtcaag agcacattaa attaaaagaa atcaggtata
    41521 agtcaaccta tgatgtgatt catggtatgg attaatgata ttgtttttct tgttgcctga
    41581 ttaaatcatt ctattgattg ttttttaaat cgcctaacca cttgatgact tattttatcc
    41641 gtaatttctc catggatttc cccataagtt ttctccgcat aggctaaaat ccacacatat
    41701 aattgctcca ggttttcacc ggattgacgg gcaatttcct cagtgccaca ataaattttc
    41761 gccgtataat gctcactcat tgattgaccc tgcactagaa cctattaact ccctttcttt
    41821 taattgtatt acttattgac caacaatgat taaaataagc caatattttc tatattgatt
    41881 cgatgattca ttcatctgtc aaaccgataa aaaagtgtta aactatagac tcttatcagg
    41941 agggacaacc gatatggcag acaaagtacg tggaaaagta aaatggttca ataaagccaa
    42001 aggatttggc tttattgaaa gtaatggcaa agattatttt gttcacttca gtgccattca
    42061 aggcaatggc tttaaaaacc ttcctgatgg agcacgcgtt ttatttaaag taggtcaagg
    42121 ataaaaaggc ccacacgccg aggaagtcga aatcgtttaa agtcaatgaa ttcttatcaa
    42181 aaaaactcat aaccatacaa actataggag tcatttatgg aacgaatgac agcggttgta
    42241 acaggaggta cgggcggaat aggtacggcc atatgccaac atctggccgc cgattatcaa
    42301 gtcattgctt gttattttaa aaatgggcaa catgaggaag caaaacggtg gcaagctact
    42361 caaaaagaag cgggttttga tgttgatatc ctttatgcgg ataccacaca ttttgctgat
    42421 tgtgaaaagt tcacggaatt ggtcatggag cgctatggac gtatcgacgt cttggtgaat
    42481 aatgctggta ttacttgtga tacgactctc aaaaaaatga cacttgagca atggcagcag
    42541 gttgttgatg cgaacttaac cagcgtgttt aatatgacgc gtaatgtgtt accggccatg
    42601 ctggataaag gttatgggcg aattattagc atttcatcaa ttaatggtcg caagggccag
    42661 ttcggacagt gcaattacgc ggcgaccaaa gccggtttat ttggatttag taaaagtctg
    42721 gcattggaag tagctaataa gggaattact gtgaatacta tctccccagg ctacatcgaa
    42781 acccctatgc tttctgccat gagagaagat gtattaaagt ctattattgc caacattccc
    42841 atagggcgtt taggtaagcc tcaagaaatt gcaaaggctg tggctttctt agcatcttct
    42901 gaatcaggat ttattacagg cgctaatctg gatattaatg gtgggcaata tatgtaggta
    42961 aagcagatga gaggacttgg cgcgcaaaat aaatacctga ctaccaatga gtgcacactt
    43021 ttccaagtct caaagtagga tttcctgcac tttttgttat ttcttgtata ctgttcaacg
    43081 atgacttgat tggttggagt ggattatttt ctatcatttt agattccata ggtggggcta
    43141 tattttgttt gaagcttatc aaagattaaa acacgacccc tgaaatttag atcatttttg
    43201 caatacgaaa actagatcac accggctaaa aaggtttaag gcctgcttgt gctaaccaat
    43261 ttatcatgat taattaattt gtaagtatca ttatggacat agtgtatcaa tcttgattta
    43321 tttgatgggt ggatctgtcc atcaaggtat ctaagaccat agaacagatc ttgattggca
    43381 gaaattaaaa gcgttaatgc ccaatcagca tggtgaaata atgaaataag ttgcgtgcct
    43441 tcgcagatcc taaaaactct tttcccgcca atacctggct aagcaccagt gggctggctt
    43501 taagtatttg ggctagttgt ttacagtcca actcactgta ggacagaatg taatcaatca
    43561 gatatttctg agtatctttt tttaattgaa acttaacatc aattacaggt tctgcaacct
    43621 cgataaaaca ttccatgtaa caacctcact ttcatattat tacggcatag tccattcgaa
    43681 aaacagtaaa ccatagctgt aatatcgata aaattagttt ttttacatgg aaaatatcag
    43741 aggtaaaatg caattttgat ttatttgata tcaattatta ttgtttgatg ccctttttta
    43801 tgcggctgtt atagtctccg ataaccctga atctgttttc tgctcaataa caggcttgat
    43861 tcttttcgca atcaagtaaa ggaaaacagc gcccaatagt gcccagatac ctagctgatt
    43921 aaacacatgc tggtaatcac cattggatag caatggattt gtggactcct ctttagtcat
    43981 ggcattagaa aaataatgtg ataacgatgc tgatacgcct gagaccatca tccaagtgcc
    44041 cataagcacg ccttgtaatt gcggtgggga aattctgccg atcatggcgt agcctactgg
    44101 tcctatgagt aactccgcaa ttgcttgagt agtaaaatgg agcatcaccc aataaacact
    44161 aacatagcca tgcttattgg cagagagtat actgctggat aacaaaaaga aggagcctgc
    44221 tagtatcaga aaggcccaga taaattgttt ggttactgat acattgtatc cttttgcctg
    44281 cagccgcgtt aatactattg ataataatgg ggcgcctata atgattacga tggcattgat
    44341 attcaaaatc cattgggtcg ccatttcata atggaagagg tgtttatcga cattgttttt
    44401 aatgaacaga gttacgccca tagggcctgt gaagtagatc atccagaata gaattgatgt
    44461 tactgtcagg attagaaaag tcataatctt ttgcttatcc aaagtcgatt tttgttggta
    44521 gccaaggtat aaaatgacaa aaagcataat aacgctgagg cctactacaa ccccattact
    44581 gaactgggct ttatgaaaac aaagtaaggt gaatgggata agagctaata taattcccca
    44641 gccaaaagat ttttttaaat tcagggaagc tttatttgta actttaatta aaggagtatc
    44701 gcgatcattc aagttcgacc aataacccca cataagcaaa agagtgatta cgtttggaat
    44761 tatgctggca tagagtagtg tttgatattg attggagtaa tcaaaaaagc cgcttatgat
    44821 atagccagcg cagaagccta cattcatata ggagtagctc aagaaaaagg ctttatctct
    44881 gcggttatca tccggtgcaa agcgttgtgt cagtatgctg ttgtatgcag tagtgtttaa
    44941 gccacatcct accagaaata aactcagtcc aagataaagc aacgacttaa ctgaaagcgc
    45001 caaaagtaaa ataccaatcg tttgtatggt tgtggtaatc aaaaataagg ctcggttact
    45061 tagatatcgt ccaccaacca ctccaccgag taaatgcaga acatagttaa atgcgagaaa
    45121 taaaccaaca atgctatttg aaagagcatt agacaaccct aattgtttag tgagaaacag
    45181 agataatgag gaatacagaa ttgcataaga gaaggttgaa aatgcctgta ttaaatacag
    45241 gggcatgatt ccttttggca ttttttctgg tgacaacata ttttttccct aaaactattt
    45301 ggaaggtgac tctaccatta aaaatggata aacttaatcc ctttctacaa ttatcctgat
    45361 tttcaaggga tgataattga tattcaattg ttgtatgggt gtttaggaat gaacaaaatt
    45421 agtagagttt catgggatga aatcaagtat caagtaaagc aagttaacga atccatatat
    45481 catgtcattg aagaaattaa accgggcaat acaatccctt tctttatagc tcgatttaaa
    45541 tttggtgaac attttggcat taaaaatcat gcctatctgc cgacaagcag cggtcaaatg
    45601 gaaaagattg acagcaagca cacagataat gaattattca atcatcttgg gtatggtaaa
    45661 aatagtctac ccttgggctt aatattggat aagttttgcg agtggcatta ttttggtgaa
    45721 gatgaacgca tctttccaga ctgtgttcag ggacctggtg caatttttaa tatgcaggtt
    45781 atttttgacg aggatcagac tgtagataat aatgtgctgt ctgtttcttc tggtgcactt
    45841 tcatcttttt tattgccgaa tattggttgt aaaaggaagc atgcgagaat tcagaaatat
    45901 tttaatgtcg ctgctccggc acctaaatca ccttatgagc attatctagt atttaaagag
    45961 atattgaagg ataaaaatac tattgataac tggtatagcg agattcttta tttttctgag
    46021 cagtttatca atgaagttaa acataatgat aaatggctta aactgaaatt atatttctcg
    46081 gagtcgttaa gaaaaaaact gactcaaaat acctatgatg cttcttgtaa cgatttattt
    46141 ctcagcgcca agagtattaa tcgtttcaga cctacaccat tcatcatgga tacagcaaaa
    46201 tacattttta atatttgtat gggatcgggt attggtgtga agccagcaat tgatgagcaa
    46261 tacttcccag ttaaaacaat tcagaaaatt tataatgaat gttatgactt agaatacaca
    46321 gcgacagtaa tggtgccgtc agctttgaat ggtcagtctg atagtgttta ctatccctta
    46381 cagtgtcctt ttgccaagat taatacattt aaaaccaatc aaagcaatag cgcttatagt
    46441 gaactggaaa tgctaaaaaa tgtgctgctg gcttatcagt cggagtttac cgcagataat
    46501 agttccgctt ttggaagtct gctttaccat gtaagtaaac aaacggaatt ttctttttac
    46561 cattataaat cttctagtaa taagaacatt aatagttctg aagaattaga aggtactgat
    46621 aacagatttg ctttttccta ttgtagcgat aaaacgcaat ttgccagcga tgcgaagtta
    46681 tttagaggtt gcgttagatt aagcaaacaa taaggctata tagatgagaa tattagaaac
    46741 agaacgatta tttctgcgga cttttcaaga aaatgatgtg gaatccttaa ttgccattag
    46801 ccaagaccaa aaagtaatgc agttttttcc agccatacct acgcgagaag aaactattgc
    46861 attcatagat agaatcatct ctcatcaaga agaaaagaat ttctcgctat atgctgttga
    46921 aattaaaaat accggtgaga tgataggttt tgtcggttta tttactgcaa catttcaagc
    46981 tcattttact cctgcgattg aaattggttg gcgtttatct tcaaaacact ggaaccaagg
    47041 ctatgcaaca gaggccgcca aagcagtcct tgattatgct tttaggaaac tagatttgga
    47101 tgaagttgtc tcatttacat ccgaattaaa taagccgtct attcgagtaa tgcaaaagat
    47161 cggattatat accaattcag aagataattt tgataaccct aaagtaacct caggtagccc
    47221 tttaatgggg catgtgttgt atagattaaa acgatcagag tatttatcaa ctcaaggcta
    47281 aatcatcaat tttcaaatcg agtaatttca ggtacttttt gctaattgcc tttttttcga
    47341 tcggcggaat atatactgtt gttattgaag aaatagctga tagctcgaga ttacgaagtt
    47401 gtatccattc tattcgggga gggtaagtag gaagggaggg cgcctcataa agacaaatgg
    47461 agtaatctgc tggatagtag ccacttagat aatcttttaa aattgataga ctgctagttt
    47521 tttttccatc agttctacca aaatttgcta cctgacaaag tattaaatgc gcgtgaatat
    47581 caatgcaccg ttcaaataat accagctctg tggcttcaat tgaaaaacat ccctgatcgc
    47641 ctggatcaat ttctagatca ctaaacaagc agtcttggga ggatactgca ggtaaaataa
    47701 ttgcttcgcc attatcctgt ttaatttgtc taacggcatt aagtgcagag tctgcaaaaa
    47761 cggtagggtg gccgtaaaat acaacgcata aaatggaaac ctttttatat tcttcaatga
    47821 tatgagttgt tagtgcctga tacgcttcaa ttcgtttttc gctactaaag tagatagaat
    47881 ccaacgattc agaatttttc gtttctcgtt gaatccattg ttttaggtta tcttcattaa
    47941 tgaggtagag taccttatcg gcactttgaa taacccgttt agtttcttcg gtcagatgag
    48001 aaatcgactt tattcccgac cctactacta taagtttatg cattaactcg ctttatatta
    48061 actatcttat tgctatcagg ctgatactgt ctttgtatcg agactatttt attagagtga
    48121 gcctgcctct gtgtattttg attagtgttt aacaataaat ttttgtggct tatcaaatac
    48181 tcatttccta cagcggcttc aagttcttcc attaaactca tttgatgtac cttttattaa
    48241 actttgatag ccattgtaca aataaaattc ttataggtat agccaaaaaa agtatttgca
    48301 ctgatatgct cattatgatg taataaatac tcgaaacgag tattttgatg taagtattac
    48361 aggagttgag atgatcaaat attttattga gcagaagtta tttattataa aggttaggct
    48421 atattcacaa acccagtgcg ttaaatttct atcacataat tttagaaatc gatctaaatt
    48481 aaagtgccaa catcagacat ctgattttac tcaaaagcta tccattaatt acttttatca
    48541 agcgtaagga ttgccttatg aaaacaatga atattttgca ggcctcacaa tttttaggtg
    48601 tacataaaga aagccttagg cgcatggctg cgaatggcca gctaccaggc gtcaaaattg
    48661 gccgaagatg gatatttata gaacaagatc ttgtgatgca tattagaaac aagtactcta
    48721 cttgcgacgc ttcgcagggt gcttatgtta ggagtaataa agaatggcac tctacaaaag
    48781 agatggagtt tggtggcttg atatcaggca ccaaggaaaa agagtacgca aaagtactgg
    48841 gactgaaata agagaagatg cgctgcggtt acatgatcaa ctgaagcatg aattgtggca
    48901 aaccgataaa attaagtcat tgccgaatag aacctggatg gatgcggtat tgaggtggat
    48961 tgatgaatcg tctcataagc gtagtctgga aacagataaa taccatctgg catggttaga
    49021 ttccttttta agagcaaaaa aactttctga tattgacaga gatttaattg agtttattgc
    49081 taagaagaag gaagcagagc atgtttcctt aaccactgtt aatagggttt tggaattggt
    49141 tcgtgcaatt ttaaatcgcg ctcataaaga gtggggttgg cttgataaga ctcctattat
    49201 taggatgcga aaaattgaga ataaacgcat ccgttggtta acgaaggaag aagctaattt
    49261 gcttttgaaa gaattacctc ctcatttaga ggcaatggct tctttcacat tagcaacagg
    49321 tcttagagag tctaatgtaa cgcaattgaa atggagcgaa ataaatttag ataaaaagca
    49381 tgcgctcata catgctgatg aatctaaatc caagcgcccc ataccagtac cgttaaacaa
    49441 acaagcaatc tcaattttaa aagcacagca gggaaggaat tcaacgtatg tatttacata
    49501 caatggaaat ccggtaactc gttgtaataa ccacgcttgg caaaaggcat taatacgtgc
    49561 aggtattaat gattttcgtt ggcatgattt acgccatact tgggcaagtt ggcatgtgca
    49621 aaatggtaca cctttacatg agctacaaca actcggtggg tggtctaatt atgaaatggt
    49681 gttgcggtat gcacaccttt caagtgcaca tctcagagct gcggctgaac gtgtttagac
    49741 acgttttggt cacagtaact tggttttttg gacacaaact agagctaagt gcttgatttt
    49801 aaatggcgcg ctcggaggga ctcgaacccc cgacaccttg gttcgaagcc aagtactcta
    49861 tccagctgag ctacgagcgc aatggggtgt ggatgatcat tttatatttc tggtcttttt
    49921 atgcatgagg gagaccagaa tggtgggccg tataggattc gaacctatga cacagaggtt
    49981 aaaagcctcc tgctctacca cctgagctaa cggccctttc agggtataac cagcaaaata
    50041 caatagttga tggtataagt ttattaaaaa ctggtgggcc gtataggatt cgaacctatg
    50101 acacagaggt taaaagcctc ctgctctacc acctgagcta acggccctaa agaggctgaa
    50161 atcataccgg attagagcag aatttcaagt ctttttagag attatttttt gtttaacaaa
    50221 caagatgtgt tatttatatg cttttttcaa acaaggaatt attattcctg acagttagat
    50281 tatcttctgt agctaaaatt aaccagtttt gatcttggac tttatctttg agagattgta
    50341 agtctctttt atcggtaaga attaacaaag tatcagctcc taaagcacca cagcctttta
    50401 ttgccaaggt ttccggataa gattttatag actgaataag aggcagacta tgttgactca
    50461 ccagatttaa ttctgtcaat ttttgagcat ataagttaat acattggatt aaatggtggc
    50521 tatgagctaa atcaaaagct tcttttgctt tgataaccaa agaggccagg taatctattt
    50581 gagatggtag ggtgacttct tgaagatgat gatgtgtcgc caatttgttg ccggtgtgaa
    50641 tgatcaagaa ggataaatct ttgaatggcc aggagaacga attaacctga tttttttgtt
    50701 tatcaatgta aacacaacct tgctgtgact gagcaatcac atcataacca ctgggtctta
    50761 accccaaccc cgaccaggac gattggtaat aggcctcaag catatggttt aatcgagggg
    50821 taatctcttg cagtgaacaa gttgccaaat aacaggctaa aaattgcgcg ctggatgcgc
    50881 ccaaaccgcc ttgaccatga taagggtctt gccaggatag gccaaaggga tggcattgat
    50941 ttttttgcca ccataatcca gcaggtgact ccggatgaat tccttctaat tttgcatttt
    51001 cagttaatgt aagagcaaag caaggggttg tcgttaatag gatagccggt tctcccgcaa
    51061 tggcagcgta ttctcctaat aaaaatgttt tagcgggaat ttgccatttc atactgcaac
    51121 aggagcttgt cggatttcag ctaataggtc ataagcgtta ttcaagctga ctcgtttgtt
    51181 cagattaagc cattcttgca atttttcttt gagcacgggc atttcatttt cattagcccc
    51241 tgcaactaat agcaaattat caatatgcaa tttcatatgg ccttgaatga tcccgtcagt
    51301 gcataaagct ttgagtgcgc caagattttg cactagtcca actgctgcga tcacctggga
    51361 gagttgattg gcagaagtga tatccatcat tcgtaaacac attttagccg tggggtgtaa
    51421 agaggttacg cctccaacag tacctactat gataggagca gttaactctc ctgtgagtat
    51481 ttcattttga taccgccagc gagtaatcgc tttgtattgt cccgaacgtg ccgcataggc
    51541 gtgtattcca gcttccacag ctctccaatc attccctgta gcaattaaaa ccggatcaat
    51601 tccattcata acccctttat tatgggtggc agcacgatag ggatctatct ctgcaaagag
    51661 tgaagcctct tgcaattttt gaccgaggat ggggtcaata ttacgaatga tgacttgagc
    51721 ggtcgttaac ttttgatcgt tcaagttgga taaaatacac atggtgactt cttcaccagt
    51781 tatttgttct atgggttgtt ttaaatattc aagtacttga ttgatgatat tggcacccat
    51841 agcatcgcag ctgttcatcg tcaaatgaat tactgccata tctagtccat cttctctctt
    51901 taaatgacgt acttgcaaat cggtaacacc accgcctcgt ttgaccatat tggcggcaac
    51961 atctttattt gctatttcta taaaatattg tcgattttta ttaaagaggt cagagaatct
    52021 ttgaaagtcc tttacttttg ccagttgaat ttgcccaaga atacattctc catgaaccca
    52081 ggtattgatt tcaccatgct gcctaatcca tttcgctgat ttggaaagag cagcaataat
    52141 agaggtttct tccactgcca aaggtattac ataatcacgt ccgtcaatat taaaattagt
    52201 agccacaccc aatggaagct gaaaatatcc aatcacattt tcaatcaatt tgtcggccag
    52261 atttaaatct ttaataccgc cttgtttaag aaatgttatg tcttcatgag tcaaggcacc
    52321 taaagcgcat aatctttgaa agcgctcttc acgtgataat tttgagaatc cgcgaaataa
    52381 ttcactggca ttagcagcta tggacatgcc ttctccttca aatcatcaag aaccaggctg
    52441 ccagtacaaa acatagcagt tttcaattca tattcaattg tattcatttg ggtaagtact
    52501 tgatctgttg aatccaaagc agcttcaagc ataggtttcg cgaagccgac tgtggtagca
    52561 ccgagcgcaa acagttttgc agcatccaaa ccattcctta cgccgccaga accccaaatt
    52621 tcgaaggagg gattaaggct aatggcgtta cgagtgcttt gtaaagtatc aatgccccaa
    52681 tttcgaaaag tatcagcagt tcgatgtctt atcggatcct tgtctgcccg atgtccttca
    52741 attcgccccc aatgcgtacc tcccacacca ctgacatcaa cagcggcaac acctatatta
    52801 tttaaacgga gcagtgtatt ttttgaaaag ccacaaccag tttcttttac tataactgga
    52861 gaattaatct ttttgactaa tgcttctaat gctgcccaac acccatgaaa attagttgtt
    52921 ccttcaggtt gaatacactc ctgtaatggg ttgcaatgaa taatcaatgc ttcagcgtgc
    52981 aaagtatcaa ttaagcgctg gattgcagaa attggggtat caatcaactg agcgataccc
    53041 aggttactaa acaggctgac catggggaaa tcacgacgta gtggctccca ctcaaatgca
    53101 gcctgtttgt cagttaattc tcttcgctgt gaaccaacac ccattgccca ttttgttttt
    53161 gagcaagctt ccattaatcg atagttaatt tctatagcat tggaatggcc ggctgtcatt
    53221 gaactaataa taaatggttt ttcaaccggt tttttaaacc gaatgctttg tatggaaatg
    53281 tctttaaaat caaggtcagg taaggcttca tgtaccaaag aaaaatgatc aaatggattt
    53341 aactcgctgc tttgattagc tggcattaag gctagttcta tgtgatcgcg cttacgctgt
    53401 tcaaattggc tgtattcgtt ttgcatgcct gaatttactt ttgaaaaata gcatcaattt
    53461 tagcatgaaa tcatcaaata gactattgtg tttttattct tcatagtgtt ttaattggaa
    53521 tcttatttta cccatgagtt agctaatatt cttttgttta gtcgttattt tgataataaa
    53581 tttaaggtaa ttatcatgaa atctaaatta caatactatt tttctacaaa cgagaatgac
    53641 gacgaatcat atattgaaga agatgagagt gatgcattca ctccaaatgg cgtaccatca
    53701 catgggtgga ctgcatggac cagatttttt tctcatcaaa acaactctcc taaatatgtt
    53761 gtcaagcaac ctaaaccaga taaagtccgt gccttgtcag atcttagaga tcaaaaactt
    53821 aataatgaat ggaacagaac acgctatcag catgaactgg atatatggaa tttggtttat
    53881 ccagataata aagcctattt gtttacagat aacggaattc gattggttct cccttattta
    53941 cccggtaaaa ctctattttc ttttactagt agggaagaag atcagttaac ctggtgtcaa
    54001 ctaaaccatg cagtattttg tgagtttaag cgctttactg aacttggatt gaagcactgc
    54061 gatttaaaat atgacaattt tttgatagaa agaaagcaag atggtacctt taaagcctac
    54121 ataattgatc tcgattcagt gggtaaacta aaatctgatg atcaggatta tgattggaat
    54181 attgtcctgg catctctatt tgacgatacg ggactaaaaa gtccagatgt ttgcccaccc
    54241 ggctatattg ctttgaaatc aatcgatgtt ccagtagaaa atgagaatga agcacgatca
    54301 aaacttgata aaataaaaaa agaactatcg caaaaaaaac ctaccttggt aagaatcaag
    54361 aattgccagc aaaataagtt ggattattat tttgtttatg gatgtttata taatgggcaa
    54421 tggggcttta ctaaacttga tgagaagatc attagttcgg ttaatctatc taatttttct
    54481 ggtgctttag ctgattgtag agctgcttct gttattggta atcatattaa attaaaacaa
    54541 ggccacatta gttacagact tgacctttat atcaactgtc tgaatgagaa aatagctgaa
    54601 ctcactctac tcaaatctca aaactcacca aaaagctgaa tatcccattt taaatcagct
    54661 cctgtttcta tcatgaaggg atcaaagcgt gctgctgtgc tataagtata gtatcctctt
    54721 cttggcaaag ccctcttagg ctatatcacc atcgctatat tttatactta ggccagcagc
    54781 gtcttatcac ctaccagcga taatagtaat aaggtctgta gtagggcctg tagtaaggtc
    54841 tgtaataagg gtaaccccaa cgataataat atggcctgta atagtacccc ctatagtaac
    54901 gaggccccca atacctatag cgtacccagt aagccatttg agtcggattg gctttattaa
    54961 ttattggatt gatatctcgg ttaactgctt tggcatttgc aggggtgact aaaatcaaag
    55021 ctacaatcaa aagactaaaa gccagcacca gttttagtaa agaggtccct ttaaagataa
    55081 aacgttccat tattttactc cttaaagcgt attctcttta agtatagact acaagggatt
    55141 ttttgattaa tttttgaaca caatcactgc tggaaatgag cataaatttt attgaggcag
    55201 taagtagttt attggggtta atgattaaaa aaatgcccga gccttttggc cgcagctcgt
    55261 tcctcaaggt tctctatctt attgtgttcc aatgttgttt tgaatagtcg ctgcgattcg
    55321 ggatgaaagt tcatattgcg aataacgtgt tctggtttaa gcaaggtatc aggtaattga
    55381 taacttaatt gattcttttt ctgtaactgt tgataaagag agttacttaa gcccaagcca
    55441 tgttgtagtg cctggtagaa gcgggatatg ggtccttgta aagcagcaat atggtcatag
    55501 attatgcctg gttgtggagc tccaccaatg ttttcaagaa ccagctttaa ctcctcgagt
    55561 ttcgccataa ataatttttg cttattcaaa accttaacca aggtatctat tggtttgaat
    55621 tgcttggttt gttcaagtga tttccttttg tcatgagatt ctttaagctg tttttgagca
    55681 ataacacgta ataacgaatc taccaaatga tgaagcatag ccagaatttg ttttaattcg
    55741 tctgttccca aagcaaaagc taatcgctcc agattgaatt caaaagaatg cttggcttct
    55801 atccctaata attgcagcag gtattgttgt aaatgaccta actggcttaa ggcattttgt
    55861 gtatccagtc tgtattgcaa aaccagaata agggatttga gtgagtccaa ttcatccaag
    55921 tcggattctt cctgtagccc tttctcctgt tgtaaagact ccagcagctg gttagattga
    55981 atgagttctg tttccgcttg tttgatgagg ttagattggg tttcaattaa cttttcattc
    56041 atgaagataa cctggatata aaagagcact atctatatta ttatgatacc atgaatcgta
    56101 agtttttgcg gtcttgtggt attttgtatt attctaggaa aattcaataa aaagaaataa
    56161 atatggaaca ggctagcgca cagattgttt tcgagcaaaa gaatgcaaca taccagtgtt
    56221 gtggttcctg gtctgttttg acccttgagg gattacctaa aaaatttatt accggctcat
    56281 tgcctgattc acagcaaata accataaacg gcgaaaaaat cagtgctttt gatagtgccg
    56341 gagctctggc attaaagaaa tgcatagatg agcttaaaaa aagaaataac cagattgaat
    56401 tagtcaattt ttctgatagc caacagcaaa tgctggaact catagagtcc aaacaagata
    56461 ttctttccta tcaattacct gctgtttcca aagaaggtgt tctttatcag attggtaaag
    56521 agtcagaaaa taaattgcat caggttgatg gtttgattat tttgatagga gatttaagca
    56581 caaggctttt cgaagcgttt gcatattggc ggcgatttca tttgcccagc attgtttctg
    56641 ttatagattc tgccgggcta aaagcgttac ctatcattgg attattgtct tttttgatag
    56701 gggtcgtttt agcttatcaa atgggtttgc aattagaaac ttatggtgcc aatattttta
    56761 ttgcctattt atctggcatg gcaatttttc gtgaatttgg acctttaatt actgcaatca
    56821 ttgttgcggg gcgcaccagt tcatcattca cagctcaaat tggcagcatg aagattaatg
    56881 aagaggtaga tgccatcttg accatgggtc tttctcccac tgaattatta gttttaccca
    56941 aagtcttggg tttattaatt gtgtttcctt tattaatttt ttgggcagat gtctttagca
    57001 tttggggggc aatgatcatg tccaaaaata tgttaaacat cggttttgaa gattttttaa
    57061 gccgcttaag agattccgta ggacttaacc aaatgatgct gggtttgtat aaagctcctg
    57121 ccttctcaat attaattgct ttagtggggt gttttcaggg gtttagagta gaatcaagtg
    57181 ctgatagtat aggaagccaa acaaccaaaa gtgttgtgca agctttattc ttaattatta
    57241 ttgctgacgc tgctttctcc atagcgtata gctggatggg tttataaatg agtgaaccag
    57301 tcattgaaat caagggaatg aaaaattgtc ttggcggtca atgggtgcac accgatatta
    57361 atctcaccgt agagaggggt gaaattctgg ctattatagg aggctctgga agtggcaaaa
    57421 ccaccatatt gcgcagtttg ctgatgttat tgaaaccttc tgcgggtagt attaaagtat
    57481 tcggtgaaga aatttataat cttgatgcgg agcaagcttt caggcttagg cgccgatggg
    57541 gtatgttatt tcaacatagt gctttatttt cagccatgac tgttctggag aatgtgatgt
    57601 tcccaatgag ggaattcact actttagatc aggattttat gcaagaactg gcacttttaa
    57661 aaataacttt ggtaggcttg tctaaagaag cggctgtgaa atatccttcc gagttaagtg
    57721 gcgggatgca gagaagagct gctgcggcaa gagcgattgc aatggatcca gagttattat
    57781 ttctggatga gcctacaaca ggattggatc ccaggggagc caggcttttt gatgatttgg
    57841 ttgttttttt aagggatgcg ttaaatttga caatagtcat ggttagtcat gatatagagt
    57901 ctttgaaaag agcaacggat cgtgttgcat ttgtaggtga tgggaagatt cttagtgttg
    57961 aacccataaa tgaattgatg aaaaataaaa acccgctaat cgctgattac tttagcaaat
    58021 tctaagaaat actataacag agagggcaat ttacttggaa tctaaaacca actacaccat
    58081 agtaggcctt gccgtactta ttctgacagc cggtctgtta tctgcgggtt tatggctctc
    58141 ggtcggattt aaccagaaag aatatacttc ctatactgtg tatctcaagg agtctgtttc
    58201 tggcctcagt atcgaatctc ccgttaaatt taatggggtg caggttggtt atgttaagga
    58261 aattaaacta aataaaaatg atccaaggca agtggaatta ttgttaaata ttgaaaagag
    58321 tacaccaata acaaccagta cttccgcaac attgatttca caaggcatta ctggcgttac
    58381 ttatgttggg ttatctgctg gatcttccga gctgactcct ttacgaaaaa tgccggggga
    58441 gccctatcct gtaattcctt ccaaaccatc tcttctgaat cagttggatg ctgttttaaa
    58501 ggaagttgct gagaatgtag gtgcggtgag tgaaaaggct caacttatct ttaatgaaga
    58561 aaacgctgat aatgtaagaa aatcattggc taatatagag cgtattacag aaataatcgc
    58621 tgataaaggt caaacaattg atagttcttt gaataatctt gacgtgtttg tagccaatat
    58681 ggcgaaggca agcaaacaat ttcctcagtt gataaaagat ttaaaaacag gtatttcaaa
    58741 attcaaatct ttagctgaca atatgagtgc tgcgggtaag gatgtttcaa aaacgatgat
    58801 agcgggcaag aatactatcg atcaaatttc ccaacaggct atacctccag cagtcatttt
    58861 attgcgacgc ttgaatgcca tttcagcgaa ccttgaaaaa gtcagcaatg aaatgaggca
    58921 aaacccttct gtgattattc gaggtacgaa agcacctaaa cttgggccag gggaataaaa
    58981 tatgagagtt ctcagtagtg tttgttttat acttggaata ttaacactga tgagttgttc
    59041 acctgttaaa gtcccagtga ccaacgaata tcaattaaca gcatacagta ccaaacaatt
    59101 gaccaggaag cccagggcca taacaataca agttacagtc cctgaagcgg tagcaggata
    59161 tcagaccgaa gaaatgcttt acatgaagaa accgtttaaa ttggaaccat ttgtaaaaaa
    59221 tgcctggacc tcacctcccg cggatatgct ctttccttta ttagtacaaa gcctgcagag
    59281 ttcagggtat ttttatgccg tcacatcaag tccctatagt gaagaagcag attatcgatt
    59341 ggatactcaa ctattgaaat tagagcaaaa ttttatcaag aaacctagtg ttcttgaatt
    59401 ttcagcaaaa atagtactaa cccatattag tgataatcaa attataggct cgcgaattgt
    59461 tagtttacaa ataccttgtt cacaagatac tccttatggc ggagttattg cggcgaatca
    59521 ggctacgttt cgatttactg caacggcaac agactttgtt gtatcgcata ttaaacgtga
    59581 ttaggaatag ccaaaaaata aaaagaattg tacaatggct ctccatgatt atcatgtgta
    59641 gtttctaaaa aataataaga agagtttaag gagattgtgg aatgaaagcc ggatcgtttt
    59701 ataaactggg actgcttgta gcaagcgctg tattggttgc agcctgttct aaaaccccag
    59761 gcagtgcgga tggtggggct gcggtaggtg atggtgatgc tactgcccaa ggtttggggc
    59821 aaatgactca ctttgctgga caagagcctg gggagtccta tacaacgcag gcaccgcata
    59881 atcaattgta tttgtttgcg tatgatgaca gcactttggc atcaaaatat ttaccttctg
    59941 ttaacgcgca ggcagaatat ttaaagactc acccaggtgc tcgagtgatg atagctggac
    60001 atactgatga gcgtggtagc cgtgaatata acgttgctct tggtgaacgc cgtgctgata
    60061 ctgtagctga aattctgcgt atggcaggtg ttagcagaca acaaattcga gtagttagct
    60121 acggcaaaga gcgcccggct aattatggtc atgatgaagc gtcgcatgcg cagaatcgac
    60181 gcgtagagtt tatttatgag gcaacaagat gattaatcgc aaaaaatcca ttatcaccct
    60241 atgttttgta ctgatgcttc ctttcgcctc atgggcagaa gcgccagtaa tagatgatag
    60301 tgaaaatttt gcaatgattg acaggcaaga ggagtatgat gctccccttg tcaatcctaa
    60361 gtacgataat cctcaaattg agagtgctga gttggatggg tctcaaaatg ataactactc
    60421 aaccgatacc tctcaatcct atgatgaacc cgcacttgtc aaagaagacc gaagtactat
    60481 cagtgacaat gcaaagttaa ttgataaaat tcagcagctt caaaaggaaa tacaagagtt
    60541 acgtggccag cttgaagtac aggcacacga tttgaaatta ttacagcagc agcaagtcgc
    60601 tttctacaag gatctggatt ctcgattatc caattcttca acttcagcaa aaaccattca
    60661 aaatgataaa ccagcaacag atgtttcatt gggttctaat tcgccagcga ctttaaaagc
    60721 agcttctccg caaatcaaag cagggccatc tacagggcca tctaatagta aaccacaacc
    60781 tgttattgct gtttccaggg ctaatcctgc agatgaacag ataagttacc tggcggctta
    60841 cgagttagtt aaaaataaac gctatgatga agcgattaag tctatgcaga cttttgtgca
    60901 aaaatatccc agaggtggtt acactgccaa tgcagaatac tggttaggtg aactgtatct
    60961 tgttaaaaag gactatccta aagccataga acattttgag attgttttac agcaatatcc
    61021 ttcatccagc aaggctgcag ccagtttgtt aaaatctggt tatgcttatg ccgagaaagg
    61081 cgacacgcaa gaagccaaaa agcgttttca acaagttgtt aaaacctacc ctgatacgcc
    61141 aacagcacaa ctggccagct ctaaattgga agcaattaat gctttatgaa acgatttaat
    61201 gaacaattaa gaattacgga gatttttcat tcattgcaag gtgaatcagt aactgtaggc
    61261 ttgcctactg tatttgttcg cctgacaggc tgcccattgc gatgtcagta ttgtgatacg
    61321 gcttatgctt tttcaggtgg tgaagttgtt gaaatagatg atattttaaa taaagtagct
    61381 tcatatcaat gtcagcatgt ttgtgttact ggtggggaac cacttgctca acctggctgt
    61441 attcctttat taagcaaact ttgtgatgca gggtactctg tctctttgga aaccagcggc
    61501 gccagagata tagcctctgt agatcaacga gtaatgatcg ttatggactt aaaaactcct
    61561 gattccaggg aagcagataa aaacttgctg tccaatttga gttttctaaa gcccactgat
    61621 cagattaagt ttgtactatg cagccgaact gattatgaat gggcatgtag tatgctgagt
    61681 gaatatcagt tggctgaacg ggtacaactc ttattttctc cgagttggaa tcaactaaat
    61741 cctactgatc ttgcaaattg gataattcag gataaattac cagttcgttt tcaactacaa
    61801 ttacataaaa ttttatggaa tgatgcacct ggtcattgac ctcaaggaga ctaactatgc
    61861 attggtttgc tcaggcacca gctaatattg ctttgatcaa atatatgggt aaaaaggatg
    61921 aaaattccaa tttacctgat aattcatctc tatcttacac cttgagtaat ttattaagtt
    61981 ccgtgaaatt agagaagtta ccgactaaaa aagacatttg ggaaccactt actattcctg
    62041 gagcaccaga atttaacctg tcagttgaag cgcaaaaacg gtttattgat cacttagtac
    62101 gattgaaaga gtattttgga tatgtcggtg ggtttttaat tcaatccagt aataatttcc
    62161 ctcatagcag tggtttagcg agttctgcct ccagctttgc tgccttgaca aagtgcgcat
    62221 caattgcatt aagtgaactc actcaaaaac ctttgccttc tattgatgag caagcacaat
    62281 ggagtcgatt aggttcaggc tcatcatgcc gttcttttta tgcaccatgg gcgttatgga
    62341 cgggcgataa agtctctgcg atcgatttgc cttataaaga tctattacac caagtcatag
    62401 taattagcag tcaggaaaaa gaaatatcat caagggtagc gcataagctg gttaaaacaa
    62461 gtccttttta tgaaacgcga agcgaaaggg ccgaggcaaa tttgaagttg ttactgaacg
    62521 cttttgaaaa caaagattgg acgagtatat accaaatttg ctggcatgaa tttctcgaca
    62581 tgcatcaatt atttaaaacc tgtgaaaaac cattttctta cattacagat aatactttgc
    62641 atatattaag cgttattgaa aaattttgga acgagaaagg tgatggcccc gtagttacta
    62701 tggatgctgg tcctaatgtt catttgcttt atagatcaga tcaaacagat ctcgcacgac
    62761 aatttaaatc ggatcatttg gtaggaaact atgatgtttt atgattttga aaccaccact
    62821 ttcggcaagt ggattttagc aggtgaacat gcggtagtaa ggggacatga agctttagta
    62881 tttcctatta aggaaaggca attaactttg aaatataatc cttcctcacc gtccttgagc
    62941 gcagaatttc aaggcattaa tggttctgat atgcatttat tattttggag cgtattagag
    63001 cgtggtatgc atttgttggg acgttcactg aatgaattag tcggtcactt ttatttggaa
    63061 accaatattc ctgttggggt tggcatgggt gcttcggcag cgctttgtgt tgccatgagt
    63121 cggtggtact gtgcgcagca aatgatacaa gataatcaat gcaatgaatt tgccaaacaa
    63181 ttagaacatc tgtttcatgg taaaagtagt ggcttggatg tagctggtgt agcatcagat
    63241 actggtgtat attttaaatc tgggctttgt actccagtaa agcaaaccat ttatccctat
    63301 tggtatttat cctcctgcaa tcaaatcggc attacttctc attgtataca gcaagttgaa
    63361 tatttatgga ataaaaatcc agaacttgct caccaagtag ataagcaaat ggtagaggct
    63421 gttcaggaag caaaagctgt tttggaagaa ggggggagta atgctttaaa ccgtttggca
    63481 aaagcgataa ataaagccgc agattgtttt ttacaatggg gtttggttag tgagagcttg
    63541 caacaacata tgaatcattt gtttgctgat ggtgcaattg ctgttaagcc aaccggttca
    63601 ggtggtggag ggtttgtatt aagcctttgg gataaaattc cctcatccca tatagagttg
    63661 attccggtct aggtcctttc aagcctaatc gacaaattga taatatactt cattttgctt
    63721 aatcatgccg agttcatatc gagcttgttc ttcaagagcc tgatcaccac tttttaactc
    63781 tttgatatct gcttctaacg ctttatttct ggcagctaat ttatcgtttt gacttttatg
    63841 agcctccaat tttttttcga gtttaatcca ttggatgata tttccatcac ctagccatag
    63901 cttatgttgt aaagcaacta aagcaacaat taagataatg aatatggggc gcatagaatt
    63961 ttctattgaa gaatgtatct aattactatt gtagtatctt attaccacga ggggcaatat
    64021 gtgttcaggg tatatagtat taaatttttt atcgaactga ttttaaattg tattttaatt
    64081 atattacctt tgatttaaat tagagctgat gccgagtttt attcagcact caacatcacc
    64141 ccaacttaaa cattaataat gctgaaagct tgaactcatc tctttagaat attttttcca
    64201 gcatagggta aagacgctaa ttcattgatt cttagtagtt gattatattt cgcagtcctg
    64261 tctgttcgac acaaagatcc ggtttttatt tggccacaac cactggccac tgcaagatca
    64321 gcaatgaagg tatcttctgt ttctcctgaa cgatgggaca tgacacatcg gtatccatta
    64381 tcgtatgcga gttttatagc ttgtctggtt tcgcttaaag tacctatttg attgacttta
    64441 attaatatgg cattagcaat tccctgagca attccttctt gcagaatttt gggattagtt
    64501 acaaataaat catctccgac gagctggact ttatttccca aaagggtggt taattgtttc
    64561 catcctgacc agtctttttc atccaggcca tcttcaatac tgactatggg ataactggaa
    64621 atgagatttg cgtagtattc tataagttga tgagaatcaa atttttgatt ttcagaagac
    64681 atatgataaa agccttcatt aaaaagctca gaagcagcaa catccaaggc aaatacaatg
    64741 tcttctccta atcgaaaacc tgccttttca atcgcctcgc taagaagatc cagcgcttga
    64801 cgattagatt gaatattagg tgcaaaaccg ccttcatcac caacagcagt atttaatccc
    64861 tgctttttta atacagattt cagtacatga aaaatttcag ttcccatttg taatgcaact
    64921 ggaaaatcag gagcgccgat tggcatgatc ataaattctt gaatatccac attattatca
    64981 gcatgagcgc ctccattcag aatattcatc ataggaacag gcatggtcat catgtcaccc
    65041 tgattaaggg tcatatacaa tggctgatta agagataatg ctcttgctct ggcacatgcc
    65101 agagaagtgg ccaggatagc attagctccc agatgagatt tgttttctgt attatctaat
    65161 tggcataaaa tgcgatctaa attttcctga tcaccaacag acaatccttg taatgcctga
    65221 tttatctctt cattaacgtg tttaactgct ttttgcaccc ctttgccagc atatcgtttt
    65281 ggatcattgt ctctaagttc acaggcttcc cggcttcctg tggaagcacc tgaaggaacg
    65341 ctggctcttc caataatacc gttttctaga atgacgtctg cctcaatagt cggattaccg
    65401 cgagaatcta atatttcacg tgcctgaatt ttatgtatgt gcatgatctt tcctaatctt
    65461 tttatattta ttaacttaaa tacctaccac cttaaggttt acaagcgcat taactaatgg
    65521 cattagtggc aaaccaagaa tagtatcctc gcttccttgt acttccttaa ataaccactt
    65581 gcctaaacct tcatattgat aagcaccaca actctgataa ggtttttctg cttgcaaata
    65641 ggcttcaatt gttttttcac ttagatgatg cagtgtcagt gtagccgttt catggtattg
    65701 ccaaagtatt ttgctctctt taacaatgca caggcaagca atttgttgat gttgctttcc
    65761 gcttaactct ctcaaatggc ttacagctgt ttgatgattc agtggtttgt taaatattct
    65821 tttatcagcg atgcataatt gatctgctgc aatgatgtag tgctctggat aatgttggct
    65881 tacatccaac gctttaattt tagccagggt aatccctaac tcaactagtt cgtcagagtt
    65941 atgccttgtt ttaatttcct cttcgttaca attcgatggt acaaccagga aatccaagcc
    66001 aagtgatttc atgagtttat gtcttatggt cgaagaggag gcgagaataa tcggtttttg
    66061 ttgtaaaaat ttagacatag atggccaccg atgccatgag taaaaagcct atgattacaa
    66121 agctgaaatc acttaaatta cagcaacgct ctaccatgac acagcgctcc agtccatgta
    66181 aagttcctaa atagagaaat gtaccggcag atgccgcgat taatataggg tcgaataaag
    66241 agttagtttc tactccatga ccaaaatacc agccaaaata aatgcctaag ggtgtcataa
    66301 ggctaaaaaa gaaaaaaaat accatacttt ttcgagtgct catcgagctt ttatttaatt
    66361 gaattgctat agccaaactt tccgcccatt tatgagtgat gatcgctaaa aatagcataa
    66421 tgatcatgga attgtattgg gtaaaaccta aagcagctcc cagcataagc gagtggacag
    66481 aaagcatggc ccaggcgagt attgcgaaag caggatgttc tgcatcatga tggtggtata
    66541 gttcctttcc caaatgttca aaccacaaga aaataaggaa tactactccg gtaataataa
    66601 acgcaaacgg atagttataa cccatttctt taaataaggt gtttgattca ggtaacatgt
    66661 gaagaagtgc tgcacccaga aaaacacctg tagccaaagt ttcacctatg ggaaaatcaa
    66721 tgtgtttatc gtctttaagt cgctttttaa atgggtacca gcctgcaatg agaatgacta
    66781 taaatatcga aatagcaaaa atgattttta aactggttgc ggacaacatg aagtgttcct
    66841 actttaaaat gcgtatatat tatcctgaaa tgaccaatcg aatctacagg gcacagcagt
    66901 attacattgt taaaatttag caattcttgc atgccttctg gatattagtt tgatagatga
    66961 tgcatctctt catgcaagcc aaagagacgc acttctttta tatcgatttg tgatttcagg
    67021 gttgagatta ctcgattatg caattttttc cattgattaa aatcatttga gcaaaaaata
    67081 tccaaactca tttttccatt taaataatgt atattccaaa ataaaatctg gggaaaatca
    67141 tggcgaatgt cattaaacaa tttttcctgg agtattgccc tgtttggtaa atgcaaggat
    67201 ggacatgaaa tttcatcgtc ttcagggtca acatggacaa tcacatcttt gacgcaatca
    67261 atttctttta ccagagtatg atgcacatgt tgagcgatat agtgcccctc ggaaacggat
    67321 attttgggtg aaaccagaat atggacatca ataagcacat cactacccat aaaccggctt
    67381 ctcaattggt gaatacgctg tactccatca atgctttgta taactcgttc aatttgagaa
    67441 aataattctg catcaacagc tgtgtctacc aactctttga cactgttcca gccatagctc
    67501 caccccattt ttataatcat caggcttacg ataacggcgg caatggcatc caggtaaaca
    67561 agccctgcca agctgccaat taatccaatc agcacaacta tggatgaagc agcatctgat
    67621 cgatgatgcc aggcattggc ggtgattagt tgggagctaa ttttttttcc tacatggcgg
    67681 gtgtagtgaa ataatgcttc gtttgcaatg attgagaagc acactatagg aatcgataac
    67741 cagtttggca tggtatggtc tgatttaagc aattcatcaa gtgcatccca ggcaattcca
    67801 cagcctgcca ggattagtaa aagggaaagt aataaagttg ctgccgtttc aattctttga
    67861 tggccatatg gatgagagtc atcagcgcta acgcttccat attttgaagc aaatacaacc
    67921 atcacatcag taattaaatc agaaatggag tggactccat ccgcgatgag agcatgagaa
    67981 tgatataaaa aaccaccaat cattttgaca acaccgagta aggcattaat aaaagcaccg
    68041 ataagggtca cttttttggc ttgccaatac ctgtcatggg ggtacattgt tgttcctttt
    68101 tcaatcacaa aaataatttg gatttttgta aataaaaatt agaagggaaa ctataccatg
    68161 ttatagcatg agttcctatt tatcttggat tatcaattat tagtactttg tcgaaaatag
    68221 gaaaagcaag tgctca
//
LOCUS       DBNCWK010000003        67299 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.003, whole genome shotgun sequence.
ACCESSION   DBNCWK010000003 DBNCWK010000000
VERSION     DBNCWK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 67299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67299
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.003"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            25..1122
                     /locus_tag="AC06IO_000137"
     CDS             25..1122
                     /locus_tag="AC06IO_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIO9873357.1"
                     /translation="MTIAILSTGDEIIHGDTLNTNAHYIAHALCSEGLPLGLQVACSD
                     KENEIIDSLNFLTEHHDIIILIGGLGPTKDDITRFALAKFTKEPLIQHADALNHIEKK
                     ANIAKISLNQGNLQQCLFPANARLFPNPYGTAMGCSYSWKGKVFILLPGPPRECLPMF
                     NQDVLPLLLQTSRHSNKQILRWRVFGLAESEIAQILEEALEGLDCQTGYRLETPYLEF
                     KVRCKRELIEKITSIIDPILAPHIISSLETKASDQLRSLILTNNEPITIIDEITGGLL
                     QSLLVKPENYHLLNFHNLNRTKLYFHCSGLEDYWSQQAPQGTTKLIIHYSNQMQEGRE
                     THQIPYRSAMVVHYAAEWLSFRLFHLINQLH"
     gene            complement(1081..2106)
                     /gene="cgtA"
                     /locus_tag="AC06IO_000138"
     CDS             complement(1081..2106)
                     /gene="cgtA"
                     /locus_tag="AC06IO_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIO9873358.1"
                     /translation="MKFVDEALIKVEAGKGGNGCLSFRREKFIPRGGPDGGDGGDGGS
                     IYFEASSDLNTLIDFRYTRQYKAENGQSGMGGNCTGKKGEDLTIKVPVGTMVYDADTG
                     ELLADISQPGVPVLIAQGGFHGLGNTRYKSSVNRSPRQTTPGSPGESRNLRLELRVLA
                     DVGLLGLPNAGKSTLIRAVSSSKAKVADYPFTTLHPGLGVVRVSPYKSFVMADIPGLI
                     EGAAQGAGLGHRFLKHLSRTCVLLHVIDIAPLDGSDPVADAKAILNELTQYNPDLLNK
                     PRWLVLNKIDMLPDAKEREEKIQSIIKGLEWKDKVFSISAIESKGTQELCYALMQLID
                     EMKESEA"
     gene            complement(2321..2599)
                     /gene="rpmA"
                     /locus_tag="AC06IO_000139"
     CDS             complement(2321..2599)
                     /gene="rpmA"
                     /locus_tag="AC06IO_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIO9873359.1"
                     /translation="MAHKKAGGSTRNGRDSNPKYLGVKRFGGQFVNAGEIIVRQRGTR
                     FHPGPGVGCGRDHTLYALVEGLVQFTTKGEKNRKYVTILPEQREEAAS"
     gene            complement(2612..2923)
                     /gene="rplU"
                     /locus_tag="AC06IO_000140"
     CDS             complement(2612..2923)
                     /gene="rplU"
                     /locus_tag="AC06IO_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIO9873360.1"
                     /translation="MYAVIKTGGKQYTVKEGDVLKIEMLPENVGNEIKFSEVLMLVDG
                     DKVTCGTPFVAKATVKAEVLDHGRHKKVKIIKFRRRKHHMKQMGHRQYYSQVKITAIG
                     K"
     gene            3166..3825
                     /locus_tag="AC06IO_000141"
     CDS             3166..3825
                     /locus_tag="AC06IO_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIO9873361.1"
                     /translation="MSTIQLEAQSRTDMGKGASRRLRRLENKVPAVIYGGSKKPMAIH
                     FSHNKVIKALETESIYSSVFDITVDGKVEHVILKALQRHPYKPIVLHMDLQRVSSKDI
                     LVKLVPVHFINEEQSPGIKAGGIVQHTMTQVEIRCQAKDLPEFIEVDMSKVGMDDVVH
                     LSDLKLPKGVQLTVDVADGSHDAPVVSIHAAKVSSTELEETPEVPASAVPTTDQGESA
                     E"
     gene            3944..4513
                     /gene="pth"
                     /locus_tag="AC06IO_000142"
     CDS             3944..4513
                     /gene="pth"
                     /locus_tag="AC06IO_000142"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIO9873362.1"
                     /translation="MAIKLIVGLRNPGSAYEQTRHNAGAWLVTALAQRHNSHFKIDKK
                     MQAELTEIDINNHPCRLVLPLTFMNHSGQTTRIISQFYKIEPGEILIVHDELDLPVGR
                     IKLKTGGGHGGHNGLRDITAQLGTGEFHRLRIGIGHPGHKDLVHQYVLSRPSMHDRQQ
                     IYDAIDRGIAIIPIVLSGDIARAMNQVNA"
     gene            4532..5623
                     /gene="ychF"
                     /locus_tag="AC06IO_000143"
     CDS             4532..5623
                     /gene="ychF"
                     /locus_tag="AC06IO_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIO9873363.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNVGIV
                     TVPDSRLDNLSDIVKPQQVLHATMQFVDIAGLVKGASSGEGLGNQFLANIRETDAIAH
                     VVRCFDNSDVVHVEGRVDPLSDIEVINTELALADMETLEKSLLKVGKNSKSGNKEAIF
                     ELKTLEKIKAHLDAGYPVRTLELTPEEEQVSKRLFLLTAKPVLYIANVDDNGYENNPL
                     LDKVQALASQENASIVALCAATEAELVELDEEDRQEFMVDLGLSEPGLNKVIRAGYEL
                     LGLQTYFTAGVKEVRAWTIRKGATAPQAAGVIHTDFEKGFIRAEVISYDDFIRFGGEQ
                     GAKEAGKLRLEGKEYIVCDGDVMHFRFNV"
     gene            complement(5620..5754)
                     /locus_tag="AC06IO_000144"
     CDS             complement(5620..5754)
                     /locus_tag="AC06IO_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873364.1"
                     /translation="MPDSILALASMPGSMLWSRNDEALEERGSLVQFLKNQQLQNTND
                     "
     gene            5983..7098
                     /locus_tag="AC06IO_000145"
     CDS             5983..7098
                     /locus_tag="AC06IO_000145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIO9873365.1"
                     /translation="MSLLKKTREQDYSILVTTQDQQRKRHLLNVISIELLILGLLLII
                     TNLCLLRAWLIISMLIVGIFILLGNLILIKKNFNLTLSGHILNILVLSIIIGGNLIVG
                     KTTMSYLGWFYVSPIVAFATLGLTGLVVYGLLAIGAMFLFVFEFYIPIYSISQEYVFF
                     LDNTNHLFIFALIFTVLYHFLVQNLQYELLLKEQNYLLIADKEKFHYLSHHDSLTNLP
                     NRSYFHAHLQTLLENTDTTKEAITLYFMDLDKFKPINDEYGHEFGDQLLLLTSKRLQS
                     CFRKNDFIARLGGDEFTAIIVHKPLDHIADALTLRIEQEFEQPFVINGQTIHCTISVG
                     IANYPRNAKNAEDLLKWADKAMYDNKRKKYQTLKQTS"
     gene            7232..8200
                     /locus_tag="AC06IO_000146"
     CDS             7232..8200
                     /locus_tag="AC06IO_000146"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIO9873366.1"
                     /translation="MTIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGG
                     KRLRPLVVLLASNACGYKGKDHISLAAMVEFFHTATLLHDDVIDESTLRRGRQTANSI
                     WGSKASVLVGDYLFTQSVNLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTL
                     DDYFDVIRAKTALLFAAAACIGPILTNAPIEIRNSLYNYGLHLGNAFQLIDDALDYCS
                     DATTLGKNIGDDLADGKATLPLIHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQ
                     TEAIPFTKRIAAQEVDSALSALEILPDSVYKEALVDLAKFALERNY"
     gene            complement(8227..8442)
                     /locus_tag="AC06IO_000147"
     CDS             complement(8227..8442)
                     /locus_tag="AC06IO_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HIO9873367.1"
                     /translation="MLLQIRDFIYREGVVSTQQLSREFHLDLTALQPMLDLWVRKGAI
                     RKCQGGANCKTACFKCRTPPDYYQSVV"
     gene            complement(8423..10678)
                     /gene="feoB"
                     /locus_tag="AC06IO_000148"
     CDS             complement(8423..10678)
                     /gene="feoB"
                     /locus_tag="AC06IO_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HIO9873368.1"
                     /translation="MTHALLIGNPNCGKTTLFNALTNANQRVGNWPGVTVEKKTGEFL
                     LGEHLIEITDLPGVYSLVANAEGISQDEQIAAQSVIDLEYDCIINVIDACHLERHLYL
                     TSQLFELGKPVVVALNMMDIAEHRGISIDTEKLESLLGCSVIPIQAHKNIGIPALQQS
                     LLHCSQKIKPLKLSLSVAAQQILNDLENQLISKGYKNSFAYYFSRRLAEGDTLIGEKA
                     FTESLLIKLQETEQNLDVLLADARYQKIHEIVTLVQKKHSDASEHFTAKLDKLVLHRF
                     LALPIFFAMMYLMFLFAINIGGAFQDFFDISTETIFVQGSGWLLQQLHAPNWVIALVA
                     NGVGKGINTTITFIPVIAAMFFFLSLLETSGYMARAAFVVDKAMRAMGLPGKSFVPMI
                     VGFGCNVPAIMAARTLDSERDRLLTVMMSPFMSCSARLAIYAVFVAAFFPSGGHNVVF
                     SLYLIGILMAVFTGYILRKTTLKGHASPLILELPAYHRPSLRRLLRETSLRLRFFVYR
                     AGKLIIPICVILGGLNAITWGGGISSGEANTDSLLSIIGQWITPLFAPMGIHQDNWPA
                     TVGLLTGMLAKEVVVGTLNSLYAQVGHVGEITAAHFDFWGGIKAAFGSIPANLSELGS
                     ALWNPVSASAADSELSQSVYGIMSRRFDGAVGAYAYLLFVLLYIPCVSTMAVIRQEAN
                     KRFMWTSIVWSFVVAYATSVVFYQGAKFLDHPQQSMVWILAMSLSLLFVLAVFRYSQY
                     GMGRQNAAANT"
     gene            complement(10675..10902)
                     /gene="feoA"
                     /locus_tag="AC06IO_000149"
     CDS             complement(10675..10902)
                     /gene="feoA"
                     /locus_tag="AC06IO_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter FeoA"
                     /protein_id="HIO9873369.1"
                     /translation="MQISELKQGDKVRLVSFGATDMQYRRRLLSLGITCGVEFSVVRM
                     APLGCPVQIEVRGTFLTLRKDEASQLVLEHL"
     gene            complement(10991..12073)
                     /gene="zapE"
                     /locus_tag="AC06IO_000150"
     CDS             complement(10991..12073)
                     /gene="zapE"
                     /locus_tag="AC06IO_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIO9873370.1"
                     /translation="MSLIEQYEAAIYRGEIDSDPEQREILEHMQRLAEDLQKKSDSWF
                     PWRKKHPIKGLYIYGPVGVGKTYLVDLFYQHIDEEKKARFHFHHFMQQIDAQLRRLQG
                     KKNPLQYIAKEMAKSIRLLCFDEFLVHDVAYAMILAELLQALHHYGVVLVVSSNTHPD
                     ELYLNGVHRERFIPAIEIIKKNCEVLNLNQKRDYRLGRQPLLEAYLYPLNEQTQEAME
                     KQFALITREEDCKGFITVQNRDIPYIKYGAKSIWFAFDVICNFPRSQLDYLEIADRFD
                     NVFLSNVYQLTESHTAQAIMFIHFIDVMYDRGIKLIISADVPAEELYVKGEMKETFKR
                     TLSRLIEMQSVDYLSRHPRRLVKEML"
     gene            complement(12070..12732)
                     /locus_tag="AC06IO_000151"
     CDS             complement(12070..12732)
                     /locus_tag="AC06IO_000151"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9873371.1"
                     /translation="MLFTDKLNTPGEHELMLEGLVGSLEAVLTVPEDADTRYVAFLGH
                     PHSLQGGTMNNKVVTTMARAFKELHIPSVRFNFRGVGRSGGHYDNGIGESEDMRKLVR
                     QWHSEQPQAQNIFAGFSFGSYVAYRTASHCEHALLITIAPPVHHYDYKEFEPPPHPWL
                     IIQGTEDEVVPFELVSEFASQSSQVLPVIEFVETGHFFHGKLLILKEKLMDAVRDQVC
                     LS"
     gene            12969..13757
                     /gene="panB"
                     /locus_tag="AC06IO_000152"
     CDS             12969..13757
                     /gene="panB"
                     /locus_tag="AC06IO_000152"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIO9873372.1"
                     /translation="MKIHDFKIKKQEQKKISMLTCYDYPSACIVAESNIDCVLVGDSV
                     AMAVHGHPTTIMATIEMMELHTQAVARGLGKQFLITDLPFLGHKSSQGHTVENVKRLL
                     QAGAQAVKIEGADKDTCQTISHLVNAGIPVMGHIGLTPQSIHQLGGYKVQGKNSEQAE
                     TLLQQAATLEQAGCFAVVIECVPQDLAKTITDSLVIPTIGIGAGPGTDGQVLVWHDML
                     GLQTSFNPKFVKKYFRAKDHFIEALNSYVQQVQQMHFPANEHSF"
     gene            13769..14527
                     /gene="panC"
                     /locus_tag="AC06IO_000153"
     CDS             13769..14527
                     /gene="panC"
                     /locus_tag="AC06IO_000153"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIO9873373.1"
                     /translation="MQIFHNLNEWIRFRNSLSPDLSLGFAPTMGNLHAGHASLFLASS
                     KENHYTVSSLFVNPTQFNNPDDYKHYPRTVDADLELMTQNGVDFCILPNENEIYTDGY
                     AYQVQENRLGQLMEGKYRPGHFNGVLTIVMKLFNLVKPTRAYFGEKDYQQLLLIQGMV
                     KALFMDIEIKSCPTVREKSGLACSSRNNRLTPSQREIADEFAKIFHQNKSSAMISKEL
                     EALGITVEYIEEFQGRRFAAVKIGDIRLIDNYLL"
     gene            14524..15057
                     /locus_tag="AC06IO_000154"
     CDS             14524..15057
                     /locus_tag="AC06IO_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HIO9873374.1"
                     /translation="MIQTNWCVITGGPSSGKTTLINHLAELGYKTAPEIARFYINQLL
                     SHDKKTLQEVKHDILWLQRKILNIMLRRERNLPKNDLIFFDRGTPDSIGYFRFHHLDE
                     AHVVRACRYLRYKKIFFCHPLPVVPDTIRDEDDITAKKISDYIYNAYVGLNYPVIELP
                     ATSIKERLEIILSHVDT"
     gene            complement(15071..16486)
                     /locus_tag="AC06IO_000155"
     CDS             complement(15071..16486)
                     /locus_tag="AC06IO_000155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIO9873375.1"
                     /translation="MFKKILVANRGEIALRIVRACKEMGIAAVTIHSEVDRFALHVKR
                     ASHSHCLSKKPLDAYLNGRRIIECAKVAGCEAIHPGYGFLSENAEFAEDCNKAGIVFI
                     GPSAPVIATMGSKIAAREAMIKAGVPTIPGSEGNLETIDDALHCAETLGYPVMLKATF
                     GGGGRGIRLCHDASQIKQQFARVQSEAEKSFGDSHIYMEKYIANPRHIEVQILADHHG
                     TVLHLFERDCSIQRRHQKLVEIAPSPQLDEATRQVLLEYAVKAAKEVGYTNAGTVEFI
                     LDKNNRPYFMEMNTRLQVEHPITEQITGIDLVQEQIRIAAGERLTISQEQIKYNGFAI
                     EFRINAEDPQNDFLPSFGRITRYYAPGGPGVRTDSAIYTGYQIPPDYDSLCAKLTVWA
                     FDWPSVLRRARRALEEMRMFGVKTTIPYYLEMIKSEEFQKGVFNTGLVEAHPEWLKYS
                     NKSLPQHKAAVIAAAIALNKT"
     gene            complement(16479..17195)
                     /locus_tag="AC06IO_000156"
     CDS             complement(16479..17195)
                     /locus_tag="AC06IO_000156"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIO9873376.1"
                     /translation="MYSSNYIYQHDDLELHGFLAHEEGIKQPQPAVIVAHDWSGRGEF
                     ACLKAKQLAEMGYIGFALDMYGQGQIGSNNDEKMALMQPLYSDRSLLRERLYAALEAI
                     KSISVVDNNRIAIIGFCFGGLCALDLARSGADIRGAVSFHGLLNKPENLAAGRIKAKI
                     LALHGYDDPMAKPEQVHAFCQEMTEANVDWQMHMYGQVKHAFTNPQAHDEHLGLIYDE
                     VAARRSWQAMSQFLQEIFNV"
     gene            17478..18344
                     /locus_tag="AC06IO_000157"
     CDS             17478..18344
                     /locus_tag="AC06IO_000157"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9873377.1"
                     /translation="MMTDSLIHIRDLLHAQFCYQMFITPIHLPIDKKYREFAKMACEF
                     IEGRRDEIIHREYPRHHVLHRFTPANKPNGKKILITHGWMSRAAYMVRLIRLLHKEGY
                     EVYAIDFPAHGEAKGIQLPWTDAIAIIKETINQFGPFYGLVGHSFGGSMILNTLNLAG
                     QLPEWQLNHKPERAILIASPTQMRTPVNKIARRFKLSGHAYLQLRQLIRQQASVDPER
                     IRLSHFISQAPDTYFLCIHGELDATINPKESINFCKYYKNARLSLLPDADHVSVLMDE
                     RVEQIALDFLSL"
     gene            complement(18476..19330)
                     /gene="rpoH"
                     /locus_tag="AC06IO_000158"
     CDS             complement(18476..19330)
                     /gene="rpoH"
                     /locus_tag="AC06IO_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIO9873378.1"
                     /translation="MSQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHS
                     EGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRL
                     VSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
                     DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDT
                     GDQGREKLLFAMEQLDERSQDILQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMK
                     KIRQYMEA"
     gene            complement(19627..20556)
                     /gene="ftsX"
                     /locus_tag="AC06IO_000159"
     CDS             complement(19627..20556)
                     /gene="ftsX"
                     /locus_tag="AC06IO_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIO9873379.1"
                     /translation="MLKRSQSLLAYHLQAAANSLNLFCRKPIATMMTVIVIAIALALP
                     TLFWVFTDNLSELTNRWQRGGHISLFLKPSLPEAEQTSLLEKVRTTEGVGQANIKSAA
                     DGLSELTQQEGMQDIMRYLPENPLPAVIEVVPALVIDSPAKLDLLSRKLQAFPQVALA
                     KLDMEWINRLHAILGFSGKAANALMALLALAVVFIIGNTLRLDIHNRQEEIKILKLIG
                     ATDPYIIRPFLYSGVWYGAAGALLAIFLVNIFILTLGVAVNQLANVYQMHYPLACLSL
                     RQILLLVLFAIILGWLGALLSVKRQLASIEPYK"
     gene            complement(20550..21200)
                     /gene="ftsE"
                     /locus_tag="AC06IO_000160"
     CDS             complement(20550..21200)
                     /gene="ftsE"
                     /locus_tag="AC06IO_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIO9873380.1"
                     /translation="MITFDQVSKRYPGGFEALSQVNFSLQKGEMVFLTGHSGAGKSTL
                     LKLIALLEWPTSGQLTVNGLKLNHLKKRDVAAHRSQLGITFQSPQLLNDRTVFDNVAL
                     PLQIQGVAYPMIAKRVHAALDMVGLLSKEKMLPVHLSVGEQQRVGIARAVVHKPALLL
                     ADEPTGNLDPKLSSEIMKIFEQFNQVGVSILIATHDLALIAGMKHRIVMLKGGRLC"
     gene            complement(21187..22254)
                     /gene="ftsY"
                     /locus_tag="AC06IO_000161"
     CDS             complement(21187..22254)
                     /gene="ftsY"
                     /locus_tag="AC06IO_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIO9873381.1"
                     /translation="MIKWFKRNQNSEPNLDTEEQQDHAESSMESRLEEPQELTAKEPS
                     PKESIFARFRKGLSKTRHQLGEGISRLLLGKKEISQELLEELETLLISADLGLDTTQD
                     VLKQLNEGLARKQLSNGDAVYQTLKTNLQTILSQEEKPLSIETEDGSPFVILMVGVNG
                     AGKTTTIGKLAKQFQKQGKKVMLAAGDTFRAAAVEQLHVWGERNGIPVIAQHTGADSA
                     SVIFDALQAAKARKIDVLIADTAGRLHTQSNLMEELKKVKRVMQKLDPKAPHETMLVL
                     DASIGQNALAQAKQFHEAVQLTGITMTKLDGTAKGGILFAIANDLGIPFRYLGVGEGI
                     EDLRPFDAAQFVSAIFDDDHI"
     gene            22317..23642
                     /locus_tag="AC06IO_000162"
     CDS             22317..23642
                     /locus_tag="AC06IO_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIO9873382.1"
                     /translation="MHKLLISLMMLLSFQAFSQVQEYTLNNGLKVLVKEDHRAPVVVS
                     MIWYNVGSADEPVGITGVSHAIEHMMFKGTSKYPVGVFSKTIAALGGQENAFTNNDYT
                     AYYEKLDADHLATSFELEADRMNNLQLNSEEFAKEIKVIQEERRLRTDNNPQALAFER
                     FLATAHLTAPYNHPVIGWMNDLKQMKVEDLKKWYESYYAPNNATLVVVGDVNPEKVHA
                     LAERYFGSIAKRPIASRKPQQEPSALGKKMVYINAPAKLPLLLIGYTVPSVKTAKNNW
                     EPYALEIIAGILDAGESARFAKHLVRGNQVATGAEAYYNLYSRYQSQFIVYGAPSQDH
                     QIKDLEKALITELEALKKAPVSNQELQRVKNQIIAQKTFEKDSIFGQAMELGLLETIG
                     LGWKNTETYTKAINEITPEQIQQVAQRYFQENNMTVAELKPIRQEEVRP"
     gene            23639..24943
                     /locus_tag="AC06IO_000163"
     CDS             23639..24943
                     /locus_tag="AC06IO_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIO9873383.1"
                     /translation="MSVLRAFIISFSVIFSHNLFANSFKTEKWQTKNGVRVVFYQAME
                     VPMLDISLAFAAGSAYDGKYFGLSALTTNLINQGNSGKDATTIAEALADTGAQFNAET
                     SRDMVVLSLRTLTSKEALQQSTKTFSQIISHPDFPKKAFAREKDQLLMAVEQTEESPD
                     DVAIQNFFKTLYQEHPYAHPVHGTVESLNAIKENQVIDFYKKYFVAKNGILVMVGAID
                     SSQAHQLAEQLTQDLPAGEPAPTIPKASQLADAEKINVPFPSSQTVVRLGQIGIDHHN
                     QNYFPLMVGNYILGGGTLVSRLGTEVREKRGLTYGIDSQFVPMLGEGPFIISLSTKNS
                     EARNALHITQDTLIKFIKNGPNKEELTSAKQYLTGSFPLSLGSNTNIANLLLRMAFYH
                     LPDNYLDTYVAKINAVTDAEIRQAFQQQVNPEKLLLVTVGQS"
     gene            24940..25485
                     /gene="rsmD"
                     /locus_tag="AC06IO_000164"
     CDS             24940..25485
                     /gene="rsmD"
                     /locus_tag="AC06IO_000164"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIO9873384.1"
                     /translation="MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDI
                     RNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPNLHLIKTD
                     TLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTE
                     FWKQLKLKQAGQVIYGLFEKL"
     gene            25560..26051
                     /gene="dotD"
                     /locus_tag="AC06IO_000165"
     CDS             25560..26051
                     /gene="dotD"
                     /locus_tag="AC06IO_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system lipoprotein DotD"
                     /protein_id="HIO9873385.1"
                     /translation="MNNNKIVIMFIFSALLAGCAGTMKFKKPPINNPSDDATIKLAEA
                     AVSVSDSMLEMAKVEKVITPPSKDNTLTIPNAYNLQARASVDWSGPIEELTARIAKAA
                     HFRFRVLGKSPSVPVLISISTKDESLAEILRDIDYQAGKKASIHVYPNSQVVELRYAK
                     IYS"
     gene            26032..26943
                     /gene="dotC"
                     /locus_tag="AC06IO_000166"
     CDS             26032..26943
                     /gene="dotC"
                     /locus_tag="AC06IO_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotC"
                     /protein_id="HIO9873386.1"
                     /translation="MRKFILSLSILLSALLVACSSRNHYGDTGSLAGLQAMADSKYTR
                     AQKKQKMGKIREMALKETALSVGAQAGLAWRAKIIDEQLNKQARNLDAIYDFNSLVLE
                     HNILPPVLLEGRNTLNLADAQSIRVSDRTYKVAKQAHFITTPPTWRQYLWMDYVKPEA
                     PNVTLLPKTKAEKEIWCIYTERGWKNGIDQANTILEENIARIKEDFGGMILYRKLLAM
                     NMVSPPYVSHTDLGVTGDGSEIHIDDRVLRITALPELNVNSAEWRAAVAKDENALERF
                     KNMEKLANQAKIVITNKSWQPIIAPVS"
     gene            26943..28076
                     /gene="dotB"
                     /locus_tag="AC06IO_000167"
     CDS             26943..28076
                     /gene="dotB"
                     /locus_tag="AC06IO_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system ATPase DotB"
                     /protein_id="HIO9873387.1"
                     /translation="MSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPI
                     FAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYR
                     YRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGS
                     GKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFAD
                     GVRNALRRKPRLIMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFS
                     GEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGDPNEVTS
                     ATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ"
     gene            28158..29885
                     /locus_tag="AC06IO_000168"
     CDS             28158..29885
                     /locus_tag="AC06IO_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIO9873388.1"
                     /translation="MMFHRKCLYYFFNLFISLILGSSLNWASINQHSTDSPTLDIKIL
                     GINDFHGQISSGRAVKNEPVGGAAVLAAYLKEAQLGMEDRSIITIMGDLVGASPPSSG
                     LLNDEPSILFINGLGNNQCKPESRMDPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPP
                     TDSWISLPNYPGASYPYVSANIVDAKSEQPLFPPYIIKRIHNTPIAFIGAVLKDAADS
                     MFPANAEGIKFLDEADSINRYIPEIKAKGVNVIIVLIHEGGNQIPYEGDTQTASAVEG
                     HIKEIVYSLGDEIDVVMGGHTHQFLNAFLPNRNGHKILVTQANSYSAAFAEVTLQIDT
                     KNHAVKNKSAKIITTYANRWPGTNPDTQALEIVKLAEDKIGPIINSHVGTAGYDLLRK
                     QNKDGESSLGNLIADAYRTVMDTDLGITNPNGIRDDLKAGEVNWGNIYSILPFSNRIV
                     IITLTGNDIYDLLEQQWMGSYINMLQISGFAYTYDSHQPLGHKIIEIRHQNKPLLREK
                     MYAIATSDFLASGNGVFSVMKRGKIIHIGQSDHDTVIAYIKSLPQPFHASIEARIKES
                     ETGLKTGVG"
     gene            complement(29927..30724)
                     /locus_tag="AC06IO_000169"
     CDS             complement(29927..30724)
                     /locus_tag="AC06IO_000169"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9873389.1"
                     /translation="MPSTYILNTNDKARERLSLQHQLYAKSSLDLLSEVGPSAAMTAL
                     EIGCGSGDMTLELAKLIGREGSLLTIDLSQDQLDHTQQRTRNYSNIRFKVWDVNHLSD
                     LGEKFDLIYCRMVLHHVADARSVILQMKNCLNSGGFIVCEEPSLFDSTFCSPPSPSYD
                     QFTQWARSCFSKNNRDFQIAHRLEQEFSSCGFEVEHCSLFQPLLRTNKEKLIYSMALD
                     DLTPQLLELGIAKREEIKQLHNELEDLAKTNSTMCWIRMHKIIAKLN"
     gene            complement(30754..31698)
                     /locus_tag="AC06IO_000170"
     CDS             complement(30754..31698)
                     /locus_tag="AC06IO_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9873390.1"
                     /translation="MKPFVYLTNQFLEPIIPMLIPDWNIIHGWKMASGADQSRVVALA
                     TTVWDQIDHSFLMQFPNLKIISHLGIGTDNIDINFLKQNHIILHSQPNAGVHDTAELA
                     IALLLALSRRVILNDRYTRNNEWVEKKPRFLGNHLLGKQLGLVGFGQIGEKIAQFAEP
                     FGLKIAYTARSQKNSPYLYCPTAANLASISDFLIICCSGGIDTQHLINKQVLDNLGTE
                     GYLINVARGSIVDQNALIDALQRHTIAGAALDVYQYEPEVPFALRQLDNVVLSPHMGS
                     STKENLNQMFQLQAKHLNQYLNEHLWQRESHVTLFEEQ"
     gene            31935..32957
                     /locus_tag="AC06IO_000171"
     CDS             31935..32957
                     /locus_tag="AC06IO_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl peptide synthase"
                     /protein_id="HIO9873391.1"
                     /translation="MPVIPVEEFDGFILKLGKNNYHFCKNETPFNNSCSANIAIDKYC
                     TSKLLEKAGIPVPKAISLNCSEFQRNLHKDKIAQLNFPLVIKPIDGSLGIGVLCNIKT
                     WEELESHLTKYFSSYQCLIIEEFHGKLNSYRVLVFNNRILGIVQRYPAAVTGDRVHPI
                     HELIKQENLNRKRINEALGEITLDEEARIKLQELGITENYIPSFGEQIVLCYTSNATR
                     GGSYVTINKKMCKQNRKLLLQAAKILDLQLVGIDVECSDIINIPIEQSNGVILEVNHK
                     PSIRIHEFPMKGTPQKVTKKIMRSFITRHPLSYLYSIYNNQPTAFYVRSSILIIIMGL
                     ILLAVW"
     gene            33231..34118
                     /locus_tag="AC06IO_000172"
     CDS             33231..34118
                     /locus_tag="AC06IO_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HIO9873392.1"
                     /translation="MNPVSILDASLRDGGHRTNFHFKDDDLKQILLPLDNSGIEYIEI
                     GYRNGSLHPIENIGKAGMCPQDYLLYCQSLIKKAKIAVMVHPHNVNEQDLLELKNCGV
                     KLIRICIAKGDLVNAVPLIKLAKNLNFETSVNIIHISYYTESELDSLIEEVSKQEPDM
                     IYFADSNGSLFPDRINSLYKKYTRQYKVSFGFHAHDNLGLAQANALAAVNAGVHFIDA
                     SLAGMGKGTGNLKTEFFIAYLHANNIKKYNLEDVLTAANYVRDALKIGQEPIEMSEFI
                     RGISDLSTADLKSYKPGKL"
     gene            34257..34964
                     /locus_tag="AC06IO_000173"
     CDS             34257..34964
                     /locus_tag="AC06IO_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873393.1"
                     /translation="MAKKRLLIIGPVSTQSSSLRQLSQNLSFLNPYYSIECLDPLVTL
                     QECPDEEYYGIWQNQLKPLIKIYDAFVGFSFGGVILQQCFSILSGEKKQIILFSTPTF
                     ADSILREKLETVISLCSQNKLEEGLSSLYQAVYAPHSIPDASLQYLNRELAIKRLIFG
                     LTKVLKTNCVSILNHCNVNHLHLIGEKSQLVNTKNVIKPSCGQLIVVPEASMRVLESN
                     PTFCKEIIFKALNHEIH"
     gene            34951..36798
                     /locus_tag="AC06IO_000174"
     CDS             34951..36798
                     /locus_tag="AC06IO_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIO9873394.1"
                     /translation="MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPATLSYGDIG
                     WEFWKKEGTAIPSRTWQLIAASDTVLLGAITSKPQREAKQELSKALRKSNPYYVSPVI
                     QLRQGLDLFANVRPCFSIDDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHNLLAESQ
                     HWQTIPADEASCALRLQSKPGLTRLFDFAFNHAMQTGMPRVTLADKPNVLRESGEFAR
                     KIFESTAQKYPKIQADILNVDAVALWLIKSPEKFGVIVAENMFGDILSDIGAGVMGGL
                     GLAPSANIGDKGSYFEPVHGSGPRMKKNCANPSAMFLTISMLLNHFGYPDRAKKIVNA
                     VMQVIKEKRFITYDLGGHSTTIDMANAVIERCARLEASCLSTDFSSVPKENLIESDTM
                     SNLLQQLMHCNSAEISDALDACGIEGGLLNIKPLSQGMKMVGPAYTIQYLPREKKETA
                     FHNAANYIDKVPKHSVIVIDNNGQTDCTVWGDLLTHTALRNNIMGTVVHGAVRDVESI
                     RSTNYPVFCTGIYMCSGKNRVYKANEQCPLFINGVTINPGDIVFADDNGVLVIPNELL
                     HEVVNKTIAIRLTEERIKAAVASGSTLEQAREDYYYEQPWLGINKKREQ"
     gene            36798..37658
                     /locus_tag="AC06IO_000175"
     CDS             36798..37658
                     /locus_tag="AC06IO_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HIO9873395.1"
                     /translation="MFLPEKLKFIDIHYHAAPDLYIRRRNAIEAGRLYHSLCGAVVLK
                     SHIGSTGIQATIAQEMGLPVFPSVVLNHINGGINYRVVIRALNEYQPLFSGKMLVDFP
                     TITGRKLQSKLSRELTHNNLKQYTRQGETLFGSKQQLRKEVIDILKMARDYPIVLTSG
                     LASREEIYRLIDACNQYDVPSLLLSQPSNPLTGLKFTELNELIKNEWLWVEQTALTFI
                     LEHQDKQDFSHVLTHIPRVIYSSDLGQTSQMDIPEWLHFSKRIFNELGLSAKRMEELF
                     LSNAIKLLDL"
     gene            complement(37786..38502)
                     /locus_tag="AC06IO_000176"
     CDS             complement(37786..38502)
                     /locus_tag="AC06IO_000176"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIO9873396.1"
                     /translation="MLVTKEVEYEDGDTVCKGFIAYHSSVSKPLPCVMVAHDWGGRGE
                     GACNKAIQLADMGYIGFAIDMYGNAKLGKDKVEKRALMTPFRENRGKLITRINAAFSK
                     VVQLPEVDSDKVAAIGYCFGGLCVLDLIRSGAPLRGVVSFHGVLLPLEGESNKPLHAK
                     VLILHGYDDPLVPIEQVNQFSIEMTERKVDWQVHVYGQTAHSFTDPNANDDEMGLHYN
                     QLADQRSWQSTQLFLQELFA"
     gene            complement(38633..41740)
                     /gene="dotA"
                     /locus_tag="AC06IO_000177"
     CDS             complement(38633..41740)
                     /gene="dotA"
                     /locus_tag="AC06IO_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotA"
                     /protein_id="HIO9873397.1"
                     /translation="MNKLAITVLLCFFPALALAESGSLSFAPPASDLSVVFLGNLFGV
                     VDGVLHGTGSQIMGNMFGVFNSAVLALGGIIIMYTLMVSTMNTAHEGQMLGQKWSSIW
                     IPLRSTFGLALLIPKASGYCMMQVFFMWVIVQGVGAADKIWEAALSYLNRGGVIIQAQ
                     SDPTKSLTDKGGKSTGIAKGAFTILSGQVCMLGLQKQLQTQRNLYLSQSNSPPCGGNP
                     TPEMNTFCRTAIPDFISTVNFVKKQSDDKSKGPFSLDMPNFDKSSPFYFLNGICGTVT
                     WNSISDLKADMGNNIKLTSSQLETAQLSRAIAIQQMYVTLSTVAQVMVNNDPAFSSTS
                     STGNSKNDFSAIAKQQFGVPYKASGQVCTEYQQVCQTWGSIPSSTGSTPGVLFNGTEF
                     LGAINDYNGIMTPTINLLKQSKSSEYDNKSRDFIQEANTKGWIMAGSYFFDLVKLNGS
                     AVKDESDFDSDTGLDNSSFDPAQLTKPFGKACQGTYALLCNWFQNKSDKLVQVQSLIN
                     GAPALSQDGVKQPDLSPDPKRAYVEGPLSSTVYGFINNSMMVQLPGQPGIKPLTFANL
                     INFKVDTSLYYMKHQDFDCGRVKILFFSFCLGRMMGDLFYNYVFRYVYNFFLAIFGEM
                     INSIVMAFLMIPLQGMKDIFIVGVQTLTQPGVNPIVALANMGTMYINFSGTLWLTLLN
                     MAVVSSLIPLFGIFIFALIMMAMPLLMAWIGTMVSIGFVTAYYIPVLPYMIFTFGSFA
                     WLIAVIEAMVAAPIVALGVTHPEGNEAFGKGEFAIMILVNVFLRPSLMIIGYITAIAL
                     SYVGVWILNAGFDHAISYIQSDQGIETWKKGDFADKNANSAYCQMYPADCKMIQAGQT
                     VANWTGSKIDTSQWDDFGSKLSGDYQSTALQGGYSGWAGVYAFFFSILIYTTMYLIIV
                     QKAFTLIAHLPDKVLRWIGGSPESFGQETMQWGEEAKGKVEKAGEATYGAEKAIGDKL
                     GAKGQEMLGKLGPQAKKVSENAGDVSGKGKNSGSQTGPSSKPDTGQQLGGSDSGPPTS
                     TPPPE"
     gene            complement(41737..42192)
                     /gene="icmV"
                     /locus_tag="AC06IO_000178"
     CDS             complement(41737..42192)
                     /gene="icmV"
                     /locus_tag="AC06IO_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmV"
                     /protein_id="HIO9873398.1"
                     /translation="MKKKSGSRIVRVITRIINVRKWVDWDRMKSFTLYLVNGVKRLFV
                     PQEPTHVESFDEAVKKLKLNEADLIIKQKALFRLSVIMVIAAFMLLIYMGYQLFYGSW
                     KATIISLVVVMIALVLAFRYHFWYYQIKQRKLGCTVKEWYRQGLLGEKE"
     gene            42305..42760
                     /gene="icmW"
                     /locus_tag="AC06IO_000179"
     CDS             42305..42760
                     /gene="icmW"
                     /locus_tag="AC06IO_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmW"
                     /protein_id="HIO9873399.1"
                     /translation="MPDLSHEASAKYWFEYLDPMIYRVITFMESVENWTLDGNPELEE
                     AMKQLGQELDDIEKIDLGLLAEEDKFIRIVGNIKSGRGLRLLQAIDTVHPGSASRVLI
                     HAEETSLSSNDPAGFFLKRNIVFERLRLLSRVFCQYRLKLVLRALEGDE"
     gene            42760..44166
                     /gene="icmX"
                     /locus_tag="AC06IO_000180"
     CDS             42760..44166
                     /gene="icmX"
                     /locus_tag="AC06IO_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmX"
                     /protein_id="HIO9873400.1"
                     /translation="MKVLPKLALANLFCFTTLSVFAQGQGYDPSYQQTSTNTSNLVTY
                     LTNLGKYLGYDITQSNKTPNPPYSQLFNSNVAQLIQSYAYNTFLGAIPVNAMSQSLMN
                     FVTNNVQGNTLINNLANSTFKYQNFSSPSSGADGKITANGIIDQSTTTAQAPTPTGIF
                     SAPQAPTAGTYLNDPVSQAVFNILGTPDYSFCMDNDQKNWKSDCKLNYQNLVMQNVIG
                     TLPPALSQSGPVFYSYEYNKPIISQLNSNSLIAPLLLDTSSSQSGQNSGLTAKSQAQQ
                     ALNFIRYASAQVTPPSLPKLSAYSELWNQATAKPNSEGYNEVQQKQAAATLSGYFNNL
                     RVYAAQTSVGVSNLYYILSKRLPQNMSGNQNSSNITSQALNEFNMATRRLFDPTASNI
                     PGQPNQQWIKQINDASPATVQKEIAVLLAEINYQMYLDRQIQERILLTNSIMLLQNLK
                     AAQPTADFSNQSSPSEQQ"
     gene            complement(44167..45810)
                     /locus_tag="AC06IO_000181"
     CDS             complement(44167..45810)
                     /locus_tag="AC06IO_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HIO9873401.1"
                     /translation="MSSLLSRRKTILVLLIGIYCAYDLMVALYTAWDFTTDDAYISWY
                     YARQLVSGKGLFWHVTLPPVEGYSNFLWLMIAALVIKLQLPLVSSMKVISCCSLGLAL
                     LFLYRLARLFCSPLLSILPVFLFSHYTGVTWWAVSGLETLFYCALSLALIWQCTLAFG
                     YQTVGDRPWPNRFSLYSTSHWIITNMLLLLLSLTRFEGIIWIIPLAVFIGCQYLRAQR
                     EQVFKDHKRLFLWVSIIFICFVLPYLAYFIWRLIYFGHWIPNSYRCKALAYGQFLAVD
                     FDYFLFITPLLVLSLPYLLSANKDCRHVLLWLPSVLYTLLLWKANPVVTYGLRLFLSP
                     FALFSILPVLGLNQLIQYFKFFRWDPEYLAAVLMLFITWFFVPGNDLNHLSKSVAEYN
                     ERTQNRMTIADLLNRKAKRGDSVLLDDCGIIPFFSREDIRFIDAQCLNNSDLTQKPFK
                     NDLILYADYLNSKVKPDWVITNYYPMEARGDYLTDLLYEKHFFKNYQLVSTLNSGFYL
                     NKNQPQGKKMIDFVYQIFKRVNKTGYNGPLELESQEPKW"
     gene            46177..48720
                     /locus_tag="AC06IO_000182"
     CDS             46177..48720
                     /locus_tag="AC06IO_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIO9873402.1"
                     /translation="MSKPYYFYVSGMNCTSCSNSIESFLRNSNLIKINTFHADMSTPD
                     PKKIVISLSEEKENEHESNWKTIKKLIDDTGFTCENYEFPQVTDQKPALPLEKTQTTV
                     QQEASQLSALFSNAKKIITSHWLLGGLGCITGVSLMILTMALGSLPLALMILLASIST
                     LLTIALGAQSYYEAWKKLVQSRTLTMDTLFSISTISVLIVSLASFFVPWLPMMFEAGL
                     LIYGFRHIGIAIEDTIKDKISSAKFQDRAPQKVRLYRHSDFKKTRIEEIQPNDIIQVK
                     PGELIPLDSVCEQDTLIYPTIITGAVIPKQFRSGEKILAGMRLANDAKPLTLRVLKTA
                     KESYLARLDESIAQSVLEKAPIEVKTGQLLSYFIPSIIALSILSGIIVGLIATPALAI
                     QCAISVLVSACPCTLGLIIPLAVKTGIHKGAEHGVQFKNSRVLQLAEQIDVVIFDLNG
                     TLTTGVPVVKEYAVLNDADLSSEHFLSICNVLEEQSKHPFGKAIQSFTQGINKQKFNV
                     ANLDHSNHSGISAEINGIQYTIGSKTLMQEIGISTSSLENQMNLVAGDSLVFLARNKQ
                     LLGYLVITDPLRKDAKQTVDALIKMGKEVHLCTGADEKTAYRYAQALGIKKVHANCVA
                     TTINTEDRSKPVYIKSLQNQGLKVAMIGDAANDANALAASDFGIAVLSQDSDEITQQH
                     ASAIIQSGTILPIANAFAISKQTVNNINQNLLISLGYNLTTVLIAGGLLVALGFVLNP
                     AVGVALMVTQACFVLLNVYRFKEQPLQHLQDKVQEKPEPAPSSYEAMKKHSPNFHTAP
                     EINHSQAIQSGKQPTNKNKSPSFWSDYFPELFEEEKNTSTEHLMNNKIG"
     gene            48971..49501
                     /locus_tag="AC06IO_000183"
     CDS             48971..49501
                     /locus_tag="AC06IO_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2692 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873403.1"
                     /translation="MPTTFEDKSSKVPSLKSLAANAVQKTNANLFFRLHSLETPPEIK
                     KGFIDKKLEALTHELTEDYQTKVEARNEKIEECSSNLSSNECFVKCSAFTITTLMAGV
                     HVGIYYILKAAAVDPSTQIAYISSIPATICFSMCVGVCLNRQITKCLSSCFTPSVPDK
                     ITVDLDELGRQSHVSP"
     gene            complement(49573..50373)
                     /locus_tag="AC06IO_000184"
     CDS             complement(49573..50373)
                     /locus_tag="AC06IO_000184"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9873404.1"
                     /translation="MDNESLLAILNNWSATNYAKGNYILREISSKFLAKISIHSNDYL
                     LDAGCGDGSFTQMLASLVPDGYVLGLDRSKTMIDYANKHCRSINVRFDIGDIQEPIIY
                     GPFDNILSFWCLHWTDLEKSLSNLYHVLKPGGKICAIFSSGKQSTLFEVLNSLQFQNL
                     YPDVAKNVVQSKMMRENYSVQCRRALDNLPFKSISFVVEHCQVELPDLGLFENFIHGA
                     PLFKDLPGKMYQDIVNSMVQFFDDYCKKKYGGKWVYETTPYFLTITRE"
     gene            complement(50497..51354)
                     /gene="legD1"
                     /locus_tag="AC06IO_000185"
     CDS             complement(50497..51354)
                     /gene="legD1"
                     /locus_tag="AC06IO_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegD1"
                     /protein_id="HIO9873405.1"
                     /translation="MILIFEEHRMQLSSEQCDFFFTNGYLVINNFFSESVCDLLKQRI
                     ETLLENNQAEIPKTIFSTQTNEHAKKQYFLDSGDKIHYFFEPGAFDEAGDCLRPFNQS
                     INKIGHALHELDPVFRQYSRDDRIKKIAHQLGLKTLGLVQSMYIFKQPGIGAEVLCHQ
                     DSTYIFGEDSDALGFWFAIEDATLENGCLEVIPSPCITPLKQRMFRRENEIYFENFDS
                     SPWPEENSVPLPVKKGALIILHGRVPHKSQANFSNQSRHAYTLHLVDISLPYPEKNWL
                     QWPNGIPCL"
     gene            complement(51443..52429)
                     /locus_tag="AC06IO_000186"
     CDS             complement(51443..52429)
                     /locus_tag="AC06IO_000186"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIO9873406.1"
                     /translation="MLVPLIRTLQNKLPNAQLTWIISKPAFYLVEGMEGVEFIVIDKP
                     KTLADYWRFRKQMLPRQFDVLLAPQASFRTNWLIPFIKAKRKVGYDSHRANDGHRLFI
                     KESITPGLEHTLEGFLKFAEPLGVTEKIIHWDLPITKADYEWAQNHLPEQERPILVVN
                     PAASKPERSWLAERYIEVLKQAQVKWDVKVVLTGGPGDHDKSLAEEIAKEIPVVNLVG
                     KTKPKQLLAVISKAKAVLCPDTGPSHMAAAVGTPVVALHAVTNPSISGPYTFQHLVVN
                     RYSQAIQTVLGVSPEKSVWGTQVHGYEAMKLITVDDVMEKLTEIFSGLMNNT"
     gene            complement(52473..52667)
                     /locus_tag="AC06IO_000187"
     CDS             complement(52473..52667)
                     /locus_tag="AC06IO_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HIO9873407.1"
                     /translation="MRKTTKEPASAKKNYVVHRHQLPLSCPTDEMELWNAHPKVYLPI
                     EKTGVEVCPYCGSRFVLQND"
     gene            complement(52690..53631)
                     /gene="miaA"
                     /locus_tag="AC06IO_000188"
     CDS             complement(52690..53631)
                     /gene="miaA"
                     /locus_tag="AC06IO_000188"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIO9873408.1"
                     /translation="MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMN
                     IGTAKPSIHELQSAPHYLIDIKDPVESYSAAQFCTDALSLCAAIIKRGNIPLLVGGTM
                     MYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQR
                     IQRALEVYYLTGSTLSTYLTGPHEQPDYCFVNLALFPEQRSWLHERIAQRFDAMLSEG
                     FIEEVQQLQAKWPIQINLPSMRCVGYRQILEYLAGHYDYGAMREKGIAATRQLAKRQL
                     TWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVSN"
     gene            complement(53624..55354)
                     /gene="mutL"
                     /locus_tag="AC06IO_000189"
     CDS             complement(53624..55354)
                     /gene="mutL"
                     /locus_tag="AC06IO_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIO9873409.1"
                     /translation="MVLRIHQLSPAIANQIAAGEVIERPASVVKELLENSLDAGASVI
                     GVDVNYGGLLQITVSDNGSGIFGDDLPLAVAAHATSKIRTLDDLYSIDSMGFRGEALA
                     SIASVAKVTIISKPEEQDNAMMLRVEGENRTLSPCARNIGTTIDVSDLFYNAPVRKRF
                     LKNEKLEFQAIEMVVKRFALSAPRIALTLKHNKKLIFSLPPALSEQAKAIRMGRILGN
                     TFMREAIFLDVEHSGMRLYGWISNHRLQRSQNDRQWIYVNQRMVKDKLLQHAVKQAYD
                     GLLHAGRFPICLLYFTLPASEVDVNVHPTKHEVRFQQPRLVHDFFTSQLTKALQSTLD
                     HSDTREYAYAASSSNEQQNTVSEPSHICNDRESHEAARALSPSWSANSLSSSVTSKGY
                     KKTSSVYHPETEFQWVILNERFILVFIKQRPYLADLVALFQEWIEKQLMQKALPLESR
                     PLLISIRYPLPNQWIHKPEQLKRELARVGIQIEYSNADDLLIRSIPLNAPYLDVRRFF
                     NILCELDFFDTEQLIALISRSQIFDPKQLTVEERIEMNQVLLELSLKEEKHNICKALT
                     IDDCRMLLHE"
     gene            complement(55345..56775)
                     /locus_tag="AC06IO_000190"
     CDS             complement(55345..56775)
                     /locus_tag="AC06IO_000190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIO9873410.1"
                     /translation="MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQ
                     GSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDV
                     KQPVKVRSSSWKPKGEFNGIRIDLVNDTPLVINNRRQLSSPVKTIKASVQQTQSKISS
                     QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAI
                     TLKLKRLIDRQPGMRAVLTRSGDYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNG
                     ASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVRSVLIDLSQTATINA
                     GLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNP
                     AYQERLSQAIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVNA
                     LQSINRLQARARLMPGQKLVIPGTWS"
     gene            complement(56775..57257)
                     /gene="tsaE"
                     /locus_tag="AC06IO_000191"
     CDS             complement(56775..57257)
                     /gene="tsaE"
                     /locus_tag="AC06IO_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIO9873411.1"
                     /translation="MAIEDNDNFITLDLVSEQESKRFAEKLAFCISAPLVITLSGDIG
                     AGKTTIIRAMLKSLGVISAIKSPTFSLVESYNCGQFHIHHFDLYRIHQEEELEYLGFR
                     DYFSNQSVCCIEWPEHGGKTLPPVDIQFNLIIKGTGRLIQIAALSVAGKRILACLAGE
                     "
     gene            complement(57382..58863)
                     /locus_tag="AC06IO_000192"
     CDS             complement(57382..58863)
                     /locus_tag="AC06IO_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HIO9873412.1"
                     /translation="MNKLENALYRCDQIKVCEQQAATLYQKDENALMALAGAEAFSFL
                     QKLYPHVRHIAVFCGAGNNAGDGYVLARLAHEQGFSVTVYQCKSIEDLPSAARNAARL
                     ALASGVHCQSADEPLDGDAELIVDALLGIGLQGAVHGVIANAIHQINASGLPVLSIDI
                     PSGLNADTGLVENICVNATATITFIGLKTGMYTLDGPDYCGVIHCAHLNLEACTTKLT
                     RFASLLRDSTLPLPLAARKKNSHKGDYGHVLVIGGGPGMPGAVSLAAKAALKTGAGVV
                     TVATWPDYAKSALPLIPEAMIWGVKTAGELTPLLAKATVCVIGPGLGDSDWAKTLFLT
                     AITTQLPMIIDASALRLLSQYPQMDDNWILTPHPGEAASLLSCTARDVQMDRYHAASA
                     IQKQYGGVVVLKGVGTVIQTAEKNIFVCPQGNPGMASAGMGDILSGIIAAFCAQGFTL
                     SDAAKLGVWVHAVAGDKVAEKSGEVGVLASDLLDEIPSILNCI"
     gene            complement(58991..59386)
                     /locus_tag="AC06IO_000193"
     CDS             complement(58991..59386)
                     /locus_tag="AC06IO_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIO9873413.1"
                     /translation="MAMTSNKPYLIRAFYDWIVDNDLTPYILVDASNPNVQVPREHVQ
                     HDRIVLNISPSATRGLLLENDRIVFTARFSGQTEQIFVAPNAVLEIYAKENGRGIAFS
                     VDEDDEPPPKTSSSSESATKNKPSLKLVK"
     gene            complement(59389..60009)
                     /gene="sspA"
                     /locus_tag="AC06IO_000194"
     CDS             complement(59389..60009)
                     /gene="sspA"
                     /locus_tag="AC06IO_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HIO9873414.1"
                     /translation="MAIVAKRTIMSLFSDVDDVYSHQVRIVLAEKGVNVEILAVKQDE
                     PGADLLSINPYGTVPTLIDRELVLYEARIIMEYLDERFPHPPLLPVYPVARAEARKMM
                     HRIEQDWYYLMNAILREESVEQARANLLESLTSLDPVFADKPYFLSDEFSLLDCALAP
                     LLWRLPKLGLEIAPNFKGIISYMQRVFKRDSFQTSLTDAERQIRAA"
     gene            complement(60259..60999)
                     /locus_tag="AC06IO_000195"
     CDS             complement(60259..60999)
                     /locus_tag="AC06IO_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIO9873415.1"
                     /translation="MMKIKVLLVAALAFLMSNAAFTENLTMDYVPVDINDKESLQRGA
                     KMFMNYCSGCHSLKYMRYNRMAQDLGLTTFDGQIAENLLKNNLIFTQATIYDPIQIAM
                     SPEDAKQWFGMVPPDLSLSARDRGPVWIYNYLKSFYSDPSRPFGANNLLMPDVAMPNV
                     LEPLIGKVILVKDKNSHANSLLVVKRGEMFEGQFDSALKDLVNFLVYVGEPAKLIRYK
                     LGVFVLLFFCIFLIAAYSLKKAYWKQLK"
     gene            complement(60996..62210)
                     /locus_tag="AC06IO_000196"
     CDS             complement(60996..62210)
                     /locus_tag="AC06IO_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIO9873416.1"
                     /translation="MNGLVNWLDERFPLVSTWKAHFSNYYAPKNFNFLYFFGSLALIV
                     LVNQIITGLWLTMFYTPNADQAFSSVEFIMRDINFGWLLRYMHSTGASAFFIVIYLHM
                     FRGLLYGSYQKPRELVWLLGMFLYLLLMAEAFFGYLLPWGQMSYWGAEVITSLFGAIP
                     YIGKGLAVWLRGDYSVANPTLQRFFALHVIGIPILMIILVFLHIVALHKVGSNNPEGI
                     DIKKALDKDGKPLDGIPFHPYYTVKDFVGIIVFSILFFAVVFFAPEMGGYFLEHANFV
                     PANPMVTPDHIAPVWYLAPFYAILRAIPDKLIGVVCMAAAILLLFFLPWLDKSNVRSV
                     RYKGMYSRISITLFAFSFLLLGYLGTTPVTPVRLFLARIAAVIYFSYFILMPIYSRFE
                     KTRPVPTRIGEK"
     gene            complement(62221..62838)
                     /gene="petA"
                     /locus_tag="AC06IO_000197"
     CDS             complement(62221..62838)
                     /gene="petA"
                     /locus_tag="AC06IO_000197"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIO9873417.1"
                     /translation="MTDLNQDVSNHNQDEQLDEERRRFLLHTTCILGGVGAACALTPF
                     ITSWLPSSKAQAAGAPVKVDVSRMEPGEQAIIEWRGKPVWIIKRSKDMLNQLQKEDES
                     QLRDPDSLVDQQPEYAQNKFRSINPEFLVLIGICTHLGCSPKYKPSLGELGPDWPGGF
                     FCPCHGSMFDLSGRVFKGVPAPINLEVPPYHFLDEHTIVIGEDAK"
     gene            complement(63012..63443)
                     /gene="rpsI"
                     /locus_tag="AC06IO_000198"
     CDS             complement(63012..63443)
                     /gene="rpsI"
                     /locus_tag="AC06IO_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIO9873418.1"
                     /translation="MAEMKQYYGTGRRKSSIARVFLRPGKGDIKVNDRTLQEYFCRET
                     SCMIVMQPLETVDLLNKFDVYITVTGGGISGQAGAVRLGIARALVAYDEDGLAEGAEP
                     STDSFRRKLRARGLLTRDSRRVERKKVGLHKARRATQYSKR"
     gene            complement(63449..63883)
                     /gene="rplM"
                     /locus_tag="AC06IO_000199"
     CDS             complement(63449..63883)
                     /gene="rplM"
                     /locus_tag="AC06IO_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIO9873419.1"
                     /translation="MKTFSAKGNEVKRDWFVVDASEKVLGRLATEIARRLRGKHKAEY
                     TPHVDTGDYIIVTNAEKVIVTGRKFKNKMYHHHTGFPGGIKSASFEKLQDKNPTKIIE
                     LAVKGMLPKNPLGREMYRKLKVYAGSEHPHTAQQPKQLEIEE"
     gene            complement(64223..64540)
                     /locus_tag="AC06IO_000200"
     CDS             complement(64223..64540)
                     /locus_tag="AC06IO_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIO9873420.1"
                     /translation="MLSHYTKHVFICTNQKAPGKQCCANSGGEEFFDFMKSKLLEFDL
                     HGPGKIRVSKSGCLGRCSSGPCIVIYPEGVWYTYSSFEDIEQIIKYHLIDGKIVAPLL
                     LDR"
     gene            complement(64542..64835)
                     /locus_tag="AC06IO_000201"
     CDS             complement(64542..64835)
                     /locus_tag="AC06IO_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIO9873421.1"
                     /translation="MNALSKAIMAETLCDELKLNKPVAKEMVENFFEELRHALENGQH
                     VKLSGFGNFTLRDKPQRPGRNPKTGEEIPVEARRVVTFKPGLKLKTKIEKIGK"
     gene            complement(64838..67219)
                     /gene="pheT"
                     /locus_tag="AC06IO_000202"
     CDS             complement(64838..67219)
                     /gene="pheT"
                     /locus_tag="AC06IO_000202"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIO9873422.1"
                     /translation="MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTH
                     VIVAEVLNTKPHPDADKLTLCEVNINKDKPLKIVCGAANVRPGLRVALAMIGAHLPGG
                     LQIKESKLRGELSQGMLCSATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDI
                     DLTPNRADCFSILGVAREVAVLNKLPLIEQPIVTVPPAIDDGLRVNLIHSEACPRYCG
                     RIIRNLNLEAKTPLWMAERLRRGGIRTLHPVVDVMNYVMLELGQPMHAFDLSKINGEI
                     NVRYSASGEQLELLDGQEVVLNDNVLVIADKEKPLAMAGIMGGANSAVQEQTQHVFLE
                     SAYFNPVTIAGVARKYGLFSDSSQRFERGVDPCLQSKALERATELILSISGGEPGPVI
                     ESFDKKFLPGTVSFLFDTTKVKKLTGLSIPLNEMKNLLEGLGVVIIKETNHFFEVTIP
                     SHRVDLQQDADLVEEIIRLYGYDKLQAQPMQTSVQAGLISAKEKIATHVSSWFSAKGY
                     HETISYSFVDPELQEALYPQKEFMELLNPISSELSQMRAGMWPGLIASMIYNSHRQQT
                     AVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGNLNWSESARLFDFYDLKGDLQSLFA
                     SLKLDDVEFIQSSHHALHPGQSAQIVINGKHSGWIGVLHPRLSDAFDLDQDVVLFELN
                     LESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQIERVIRNTVKEDWLKSFDVFD
                     VYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAIIKKLENEFSILLRE"
BASE COUNT        21204 a        14384 c        12338 g        19373 t
ORIGIN      
        1 aaattactca ttggaataca caaaatgaca atagctatct tgtctaccgg agatgaaata
       61 atacatggcg atactctaaa taccaatgcg cattacattg ctcatgccct ttgctctgaa
      121 ggcttacctc ttggattaca agtggcctgt agcgataagg aaaatgagat tatagatagc
      181 ctaaacttcc tgacggaaca ccatgatatt ataattttaa ttggtgggct tggtccaacg
      241 aaggatgata ttactcgctt tgctttggct aaattcacca aagaacctct tattcagcat
      301 gctgacgctc tgaatcatat agaaaaaaaa gcaaacatag ccaaaatatc actgaatcaa
      361 ggcaatttgc agcaatgcct ctttccagcc aatgcacgac tttttcctaa tccttatggc
      421 acagccatgg gatgttctta ttcatggaaa ggtaaagttt ttattctatt gcctgggcct
      481 cctcgtgaat gccttccaat gtttaatcag gatgtactgc cgttattatt gcaaacatcc
      541 aggcatagca ataaacaaat tctgcgctgg cgagtgtttg gcttggctga aagtgaaata
      601 gctcaaatcc ttgaagaagc tctggaaggt ttagactgtc agacagggta ccgattggaa
      661 acaccttatc tggagtttaa ggttcgctgc aaaagagaat tgattgaaaa aataacatca
      721 ataatagacc ccattttagc cccgcacatt atatcatcac ttgaaaccaa agcatctgat
      781 caattgagaa gcctgatctt aacgaataat gaaccaataa ctattattga tgagattacc
      841 ggaggattat tgcaatccct attggtaaaa ccagaaaact accatttatt aaactttcat
      901 aatcttaaca ggaccaaatt atattttcat tgcagtggat tagaagatta ttggtcacaa
      961 caagcacccc aaggcacgac caaattaatc attcactaca gcaatcaaat gcaagagggt
     1021 cgtgaaacac atcagattcc ttatcgaagt gcaatggttg tgcattatgc cgctgaatgg
     1081 ctaagcttca gactctttca tctcatcaat caattgcatt aaagcgtagc aaagttcttg
     1141 tgttccttta ctctctatag cggaaataga aaatactttg tctttccact ccaagccttt
     1201 tattatggat tgaatttttt cttctctttc ctttgcatca ggtaacatat cgattttatt
     1261 taaaaccaac catctgggtt tgttgagcag atcgggatta tattgagtca actcattaag
     1321 aattgctttc gcatcagcaa caggatcact cccatccaaa ggcgcaatat caataacatg
     1381 cagcaagaca caggtacgtg aaagatgttt taaaaaccga tgccccaatc ctgctccttg
     1441 agccgcgcct tctataagac ctgggatatc cgccatgaca aaactcttat agggtgaaac
     1501 gcgaaccacc cctaaacctg gatgtaaggt tgtaaatggg tagtcagcca cttttgcttt
     1561 tgaactggag acggctctta ttaaagtaga ctttcctgca ttgggaagcc ctaacagacc
     1621 tacgtctgct aaaactcgga gctccaaacg caaatttctg gattcacccg ggcttccggg
     1681 tgttgtttgt ctaggtgatc gattcacgct gctcttatag cgagtgttac ccaaaccgtg
     1741 aaacccaccc tgagcaatca gcacgggaac acctggttga ctgatatcag ctaacaattc
     1801 acctgtatcg gcatcataaa ccatagtacc gactggcact ttgatggtta aatcctctcc
     1861 tttcttccca gtacaatttc cacccatccc tgattgtccg ttctctgctt tgtattgacg
     1921 agtataacga aaatcaatca aggtatttaa atcgctgctc gcctcaaaat aaatactgcc
     1981 tccatcaccc ccatcacccc cgtcagggcc accacgagga ataaattttt ctctcctgaa
     2041 gcttaagcaa ccattccctc cttttccggc ttcaacttta ataagtgctt catcaacgaa
     2101 tttcatgcct aaccttagac taaaatagga cttacgcaaa attcaaagct taaggcaaaa
     2161 aatattttca atgaatgcgt tcagataagc taattgactg aattgaaaat attttttagc
     2221 aaagagattg ggattttgct taagtctgta aaaaaaaacc ccgtttccgg ggttaaacaa
     2281 aacgaattat gatgccattg ggtaatagca ttgtttcagc ttaactcgca gcttcttctc
     2341 gttgctccgg taatatagtg acatacttac gatttttttc acccttggta gtaaactgta
     2401 ctaacccctc taccaacgca tacaaagtat gatctcgacc acaaccaaca ccaggtccag
     2461 gatgaaaacg tgtgccacgc tgccttacaa tgatttcacc ggcatttaca aattgaccac
     2521 caaaacgttt cacaccgagg tactttggat tcgagtcgcg gccattacga gtactaccac
     2581 ctgctttctt atgagccatt gctatctcct cttatttacc aatcgcagtg attttaactt
     2641 gcgaataata ttgtctgtgc cccatttgtt tcatatggtg cttacgtcga cgaaatttaa
     2701 taattttaac ttttttgtga cgaccatgat caagcacttc ggcctttaca gttgctttag
     2761 caacaaaagg tgtgccacaa gtgaccttat caccatccac taacattaat acttccgaaa
     2821 atttaatttc attgccaaca ttttcaggca gcatttcaat ttttaaaacg tcaccttctt
     2881 tcacggtata ttgcttaccg ccagttttaa ttaccgcata cataactatt ctccaattcg
     2941 cctgattggc tatcacaatt ccaacaaagt gccatattct atgaaattat gactattact
     3001 tcaagttcat tttaaaatat tgtatacttc accacccttt accgattgac ttaatgtttt
     3061 tgaggctttc gagtaaattt taatctggtc gcggattaat taggagctat agtcatattt
     3121 aagatagtgc ctagagggag cccttaaatt tttggagttt ttataatgtc aactattcaa
     3181 ttagaagcac aatcaagaac ggatatgggg aaaggtgcga gccgccgcct acgtcgtctt
     3241 gaaaataaag ttcctgctgt catatatggc ggaagcaaaa aacctatggc aatccatttt
     3301 agccataata aagtgattaa ggcattggaa acagaaagca tttattccag cgtttttgac
     3361 atcacagtag atggcaaagt agagcacgtt attttgaaag ccttgcaacg ccatccctac
     3421 aaaccaatcg tgttacatat ggatttacaa cgcgtttcca gtaaagacat tctggttaaa
     3481 ttagtacctg ttcacttcat aaatgaagaa caatctccag gaattaaagc tggcggtatt
     3541 gttcaacata ccatgactca agttgaaata cgttgccagg caaaagatct gcctgaattt
     3601 attgaagtag atatgtctaa ggtaggtatg gatgatgtag ttcacttatc ggatttaaaa
     3661 cttccaaaag gcgttcagct gacagtggat gtagccgatg gtagtcatga tgctcctgtg
     3721 gtgagtatac atgccgctaa agtaagttca actgaactgg aagaaacacc tgaagttcca
     3781 gcatcggctg taccgactac agatcaaggg gaaagtgctg aataagcact tccctgttat
     3841 atatttatgc aaatattcag agcttaatcc agagtatttg cataaaatct agtgctagaa
     3901 tagtcaacac aaatccgctt aattcaaatt ttatagaaaa tctatggcca ttaaactaat
     3961 tgtcggttta cgcaatcccg gatcggctta tgaacaaacc agacacaacg caggtgcgtg
     4021 gttagtgacc gctttggccc aaagacataa ttcgcatttt aagattgaca agaaaatgca
     4081 agcggaatta actgaaattg atattaacaa ccacccatgt cgactagtgc ttcctttgac
     4141 ttttatgaat catagtggtc aaacaacaag aataatcagt caattctata aaattgaacc
     4201 cggtgaaata ttaattgttc atgacgagtt ggatttacct gttggacgaa tcaaattaaa
     4261 aacaggtggt ggccatggtg ggcataatgg tttgagagac ataactgctc aattgggaac
     4321 aggtgagttt catcgattac gtatagggat aggtcatcct ggacataaag atctagtcca
     4381 tcaatacgta ctcagcaggc cttctatgca cgacaggcaa caaatttatg atgcaataga
     4441 cagaggtata gcgataatac ctatagtatt gtcaggggat atagcccgag caatgaatca
     4501 agtaaacgct taacataatg aggtatgact catgggattt aaatgtggaa tagtaggatt
     4561 gcccaatgta gggaaatcga ctctttttaa tgcattgact aaggctggga tagaggcggc
     4621 caattaccca ttttgtacca ttgagcccaa tgtaggtatc gtcaccgttc ctgattcaag
     4681 actggataat ttaagcgaca tagtcaagcc tcagcaggtg cttcatgcca ccatgcaatt
     4741 tgttgacatt gcgggattgg taaaaggtgc ttccagtgga gaaggattag gaaatcaatt
     4801 tttggccaac attagagaaa cagacgctat tgctcacgtc gttcgctgct ttgacaactc
     4861 ggacgttgtt catgtggaag ggcgagttga ccccttaagt gacatagagg tgattaatac
     4921 agaactggca ttagcggata tggagacatt ggaaaaatcg ctattaaaag tagggaaaaa
     4981 cagtaaaagc ggaaataaag aagccatttt tgaattaaaa actctggaaa aaattaaagc
     5041 acatcttgat gccggatatc ctgtaagaac ccttgaactt acccctgagg aagagcaggt
     5101 aagcaaacgc ctttttcttt taacggccaa accagtactt tacatagcca atgttgacga
     5161 taacggctat gagaataacc cgctcctgga taaagttcag gctctggctt cccaggaaaa
     5221 tgccagcata gtagcccttt gtgcagcaac agaagccgag ctggttgagc tggatgaaga
     5281 agacagacaa gagtttatgg ttgatttggg gcttagtgaa cctggattaa ataaagtgat
     5341 ccgtgccggg tatgaattgc tgggattgca aacctatttc accgcaggag ttaaagaagt
     5401 aagagcctgg acaatacgca aaggtgccac agcaccgcaa gctgccggag tcattcatac
     5461 cgactttgag aaaggattca tccgcgcgga agtgatttct tacgatgatt ttattcgctt
     5521 tggcggagaa caaggcgcca aagaagctgg aaaattgcgg cttgaaggca aagaatatat
     5581 tgtctgtgac ggagacgtca tgcattttcg ctttaatgtt taatcattag tattctgcaa
     5641 ctgctgattt ttcaagaatt gaaccaagga gcctcgctcc tctaacgctt catcattgcg
     5701 tgaccagagc atcgagccag gcattgaagc aagtgctagt atgctatcag gcatgtattt
     5761 tcctactctt ataccacact gactgttgtt tagcttgcga tagctcaact cgacgccgag
     5821 tcaacagaag ataaacgatt tggtagaaaa tgaatagcaa accaactcct tgtgcgtaaa
     5881 tcaacctcaa cgagagcagt gtggctgttt tttaatctat aattgaatta ataaaggaaa
     5941 ttagccccat tgattaccgg ccaatccgat tgaggtaggt gaatgtcatt actaaagaaa
     6001 acaagagagc aggattactc aatcctggtt accactcaag accagcaaag aaagcgccat
     6061 ttacttaatg ttatttccat agagctttta attttgggtc ttttgctgat aataacaaac
     6121 ctttgcctgc taagagcctg gcttataatc agtatgttaa tcgtgggcat tttcatcttg
     6181 ctcggaaatt taatcttaat caaaaaaaac ttcaatctga ccttgagtgg gcacattttg
     6241 aatattcttg tgttgtctat catcattgga ggcaatctca tcgttggaaa aactaccatg
     6301 tcttacctgg gatggttcta tgtttctcca atcgttgctt ttgctacact tggattaacc
     6361 ggcttagtcg tctatggcct gctggcgatt ggggcaatgt ttctttttgt attcgaattc
     6421 tacatcccca tctattccat ttctcaggaa tatgtatttt ttctggataa taccaaccac
     6481 ctgtttattt ttgcccttat tttcactgtc ctttatcatt ttctggttca aaacttacaa
     6541 tatgaattgc tgttaaaaga gcaaaattac ctgctcattg ccgataaaga aaagttccat
     6601 tacctttcgc atcacgactc actaaccaac ttacccaacc gctcctactt tcatgctcat
     6661 cttcagaccc tattagagaa tactgacact acaaaggaag ccattactct ttattttatg
     6721 gatttggata aattcaagcc catcaacgac gaatacggtc atgagttcgg tgatcagctt
     6781 ttacttttaa ccagcaagcg tttgcaatcc tgctttagga aaaatgattt tatagcccga
     6841 ctgggaggag atgaatttac tgcaattatc gttcataaac cccttgatca cattgctgat
     6901 gctctaactc ttagaattga acaggaattt gaacaacctt ttgtcataaa tggccagacc
     6961 attcattgca ccataagcgt tgggatagcc aattacccgc gcaatgcaaa aaatgcggaa
     7021 gatttgttaa aatgggctga taaagcgatg tatgacaata aaaggaaaaa atatcaaacg
     7081 cttaaacaaa cctcttaacc aacattccta atttaatcag tatgattggg tcttcatttt
     7141 ataaagcaca ggatgcagca atctgacatt cacttgacaa ttaactttta caactttagc
     7201 attcattgat taaatatcag agggtaaatg aatgactatt gaccgtatac gagcgctggt
     7261 tagtgaagat tttaacgcgg ttaataattt aattattgat aaaattcaat ctcaagttgg
     7321 tttaattcag gacttgtcac aacatatagt cgaaagtggc ggcaaacgtt tacggccttt
     7381 ggttgtcctg ttggcaagca atgcttgtgg atacaaagga aaagaccata tttctcttgc
     7441 cgctatggtt gagtttttcc atactgccac tttgttgcat gatgatgtga ttgatgaatc
     7501 cactttaaga agaggccgac aaactgcaaa tagcatttgg ggcagtaaag ccagtgtttt
     7561 agtcggtgat tatcttttta cccaatccgt aaatctcatg gtagaggttg gcattaaaga
     7621 aattctcaat ctactggcca atacttccca ccaaattacc tgtggtgaag tcaaacaact
     7681 tgccaatcgc cacaatcctg ctttaaccct tgatgactat ttcgacgtca tccgggccaa
     7741 aacagctctt ttatttgctg cggctgcctg cataggccct atactcacaa atgcgcctat
     7801 tgagattcga aatagtttat ataattacgg tttacacttg ggaaatgcct ttcaattgat
     7861 tgatgatgcc ttggattatt gctcagatgc taccacttta ggtaaaaaca ttggcgacga
     7921 tttagctgat ggaaaagcga ctttaccact gatacatgcg ttacaaaatg gtaatgcgac
     7981 tcaacaacag caaataagag aaagtttaat gaaaggcagt ctaagccatc tgcctgatat
     8041 tttaattgct attgagcaaa ctgaagccat accgtttacg aaacgaattg ctgcccaaga
     8101 agtagactca gcactatctg ccttggaaat actgcctgac tcagtttata aagaagcttt
     8161 agtcgatttg gccaaatttg ccttggaaag aaattattaa cctgatagtt gcatcgattc
     8221 taataattac acaaccgatt gataataatc tggtggagtg cggcatttaa agcaggcagt
     8281 tttgcaattg gctcctccct gacacttcct tattgctccc tttctaaccc ataagtccag
     8341 cataggttgc aatgcggtca gatccaagtg aaactcccta ctcaactgtt gagtgcttac
     8401 taccccttcc cggtagataa aatcacgtat ttgcagcagc attttgcctc cccataccat
     8461 actggctata acgaaataca gctaaaacaa aaagcagact cagtgacata gccaaaatcc
     8521 agaccatact ttgttgagga tgatccagaa atttagcgcc ttgataaaaa acaacagaag
     8581 tagcgtaagc cacgacaaat gaccaaacaa tggaagtcca cataaatcgc ttgtttgcct
     8641 cctggcgaat caccgccata gtcgaaacac aagggatata aagcaaaaca aaaagcaaat
     8701 aagcgtaggc tcctacagct ccatcaaaac gtcgtgacat aatgccatag acagattgag
     8761 ataactcact atcagctgca ctggcagaaa cagggttcca caatgcagag cctaattcag
     8821 acaaattggc gggaatagac ccaaaagcag ctttgatgcc cccccaaaaa tcaaagtgag
     8881 cagcagttat ttctcccaca tggcctactt gggcataaag tgagtttaag gtgccaacca
     8941 ctacctcttt agccagcatg ccagttaata aaccgacagt cgcgggccag ttgtcttgat
     9001 gaattcccat tggagcaaat aatggagtaa tccactgacc tattattgac aataaagaat
     9061 ccgtattggc ttctccggag ctaatccctc ctccccaagt aatcgcattt aatcctccca
     9121 gaatcacaca aatgggaata atcagcttac ccgcacgata aacaaaaaat cgtaatcgca
     9181 aggaggtttc tctcagcaat cttctgagtg agggtctatg ataagcgggt aactccaaaa
     9241 tcaaaggaga agcatgtcct ttcaacgtag ttttgcgtaa aatatatccg gtaaatacgg
     9301 ccattaaaat gccaatcaaa tacaacgaaa aaacaacatt atgcccgcca gagggaaaaa
     9361 aggccgcgac aaatactgca taaatagcca atcgggcact acaagacata aatggactca
     9421 tcatcacagt taataatcga tcgcgctccg agtccagggt acgtgctgcc ataatggcag
     9481 gaacattaca tccaaaaccc acaatcatgg gtacaaatga ttttcctggt aatcccatgg
     9541 ctcgcatcgc tttatccacg acaaaagcag ctcttgccat atagccagaa gtttccaaca
     9601 aggacaaaaa gaaaaacatc gcggcaatca caggaataaa agtaatagtg gtattaattc
     9661 ctttgcctac cccattagcg acaagcgcga ttacccaatt tggtgcatgg agctgttgca
     9721 acaaccaacc actaccctga acaaagatag tctctgtact aatgtcaaaa aaatcctgga
     9781 atgctccccc gatattaata gcaaacaaaa acatcaaata catcatcgca aaaaatatgg
     9841 gtaaagctaa aaagcgatgc aaaacaagtt tatccaattt agcagtgaag tgttcgctgg
     9901 catcactatg ctttttttga accaacgtaa caatttcatg aattttttga tagcgagcgt
     9961 ctgccagtaa cacatcaaga ttttgctccg tttcttgaag ctttataaga agactctcgg
    10021 taaaagcttt ctctcctatc aaagtatccc cttcggcaag cctacgggag aaatagtagg
    10081 caaaggaatt tttataccct tttgagatca gctgattttc caggtcattc aaaatttgtt
    10141 gtgctgcgac cgataaggaa agttttagag gttttatctt ttgggagcaa tgaagtaatg
    10201 attgctgaag tgccggaata ccaatgtttt tatgggcctg aatgggaata acagaacacc
    10261 ctaacaaact ctcgagtttc tctgtatcaa tagaaatacc ccgatgctcg gcaatatcca
    10321 tcatgttaag tgctacaaca acaggtttac ctaactcaaa aagttggctg gttaaataca
    10381 aatgtctttc aaggtgacaa gcatctataa cattaatgat gcaatcgtat tccaaatcaa
    10441 taactgactg agcagcaatt tgctcatcct ggcttattcc ttcggcattc gcaaccagag
    10501 aatagacccc tggcaaatca gtgatttcaa ttaaatgttc tccaagcaaa aattcacctg
    10561 tttttttctc aacagttact ccgggccagt tgcctacgcg ttgattcgca ttggtcaagg
    10621 cattgaatag ggttgtctta ccacaattgg gatttcctat taatagggca tgagtcatag
    10681 atgctccaac actaattgac tcgcttcatc tttacgcaaa gttaaaaatg ttccccttac
    10741 ttcaatttgt accggacacc ctaatggagc catcctcact acagaaaact caactccaca
    10801 agtaataccc agggataaaa gcctgcgtcg atattgcata tcggtcgcac caaaactgac
    10861 caatcgtact ttatcccctt gttttaattc actaatctgc ataatatata aacaacacgg
    10921 tgattcattt aattaatttt aacacaaatg atatcgagaa tcattctcaa ttaagaaatt
    10981 ttataccaca ttacagcatt tcctttacca aacgccgagg atgacgactc agatagtcca
    11041 ccgattgcat ctcaattaga cggctcaacg ttcttttaaa cgtttccttc atttcacctt
    11101 taacgtacaa ttcttctgca ggaacatccg ctgagataat gagcttaatg cctctatcat
    11161 acataacatc tataaagtga ataaacataa tcgcttgtgc tgtgtgactt tcagtaagct
    11221 gataaacatt actcaggaag acattatcaa agcgatcagc aatttctaaa taatccaatt
    11281 ggctacgagg aaaattacaa atcacatcaa aagcaaacca aatagacttt gccccgtatt
    11341 taatatatgg aatatcacga ttttgtactg ttataaaacc tttgcagtct tcttcccggg
    11401 ttattagagc aaactgtttt tccattgctt cttgtgtttg ctcatttaaa ggatataaat
    11461 aagcctctaa caagggttgt cgacctaaac gataatctcg tttttgatta agattcagaa
    11521 cctcacaatt ttttttaata atctcgatag ccggaataaa tctctctcta tgcaccccat
    11581 ttaaatacaa ctcatccgga tgagtattcg aagaaacaac caaaacaacg ccgtagtgat
    11641 gcagtgcttg taataattca gccaaaatca ttgcataagc cacatcgtga actaaaaact
    11701 catcaaaaca cagtaaacga atcgatttgg ccatttcttt ggcgatatac tgcaaaggat
    11761 tttttttgcc ttgtaatcgt cttaattgag catcaatttg ctgcataaaa tgatgaaaat
    11821 gaaatctcgc ctttttttcc tcatcaatat gctgataaaa caaatccacc aaataagtct
    11881 tgccaactcc cacaggccca tagatataaa gccctttaat gggatgcttt tttctccagg
    11941 gaaaccaaga gtcactcttc ttctgtaaat cctcagcaag cctctgcatg tgttcaagga
    12001 tttctctctg ctcaggatcg ctatctattt cacctctata aatcgcggct tcatactgct
    12061 caatcaaact catgacaggc aaacctgatc acgaacagca tccattagtt tttcttttaa
    12121 tattaaaagc ttaccatgaa aaaaatgacc cgtttcaaca aattcaatga ctggcaatac
    12181 ctgcgatgat tggctggcga actcactcac cagttcaaat ggcacaacct catcttctgt
    12241 gccttgtata ataagccagg gatgaggagg tggctcaaat tctttgtaat cataatgatg
    12301 cacaggagga gctatcgtta ttaacagagc atgctcacaa tgagacgccg ttctataagc
    12361 cacataagat ccgaaagaaa agcccgcaaa aatattttgg gcttgcggtt gctcagaatg
    12421 ccactgcctc acgagcttgc gcatatcttc actctcgcca ataccattat cataatggcc
    12481 tccagaccgc cctacacctc tgaaattaaa ccgtactgaa ggaatatgca attccttaaa
    12541 agctctggcc atcgtggtaa ctactttgtt attcattgtc ccaccttgta aagaatgtgg
    12601 atgaccaaga aaagcaacat agcgagtatc cgcatcttca ggaacagtca gtacggcttc
    12661 cagtgagcca actaatcctt caagcattaa ttcatgctct ccgggagtat ttaatttgtc
    12721 ggtaaatagc ataacaaccc tgccaattta aagataataa cccaaatcaa tatggattta
    12781 agcaaaagaa ttaggctatt atagcaatgg atgcgttgac ccccaataga ccttttgtat
    12841 atctggttta ttttgttggc agcgactttt attgaccttc agaggaggac ttgatccatg
    12901 agacaggtac ctgtattacc atcattctta tcaattccag gcgcattctc ctattcaggg
    12961 agagcggcat gaaaatccat gattttaaaa taaaaaagca ggaacaaaag aaaatttcca
    13021 tgttgacctg ttacgattac ccttcagcat gcatagtagc agaatcaaat atcgattgtg
    13081 ttctcgtggg tgattccgta gccatggcag tacacggcca ccctacaacc attatggcca
    13141 ctatcgaaat gatggaatta catacccagg cagtagctcg tggtttaggc aaacagtttt
    13201 tgattactga cttacctttt ctcggacata aatcatctca aggtcataca gtagaaaacg
    13261 taaaaaggct gttacaggct ggagcccaag cagttaaaat cgagggcgct gataaagaca
    13321 cctgccaaac gatttcccat ttagttaatg ccggcatccc tgttatgggt catatcggct
    13381 taactcccca atccattcat caattaggcg gttataaagt ccagggaaaa aacagcgaac
    13441 aggcagaaac tctattacaa caggcagcca ccctggaaca agccggctgc tttgcggtag
    13501 tcattgaatg tgttccgcag gacttggcaa aaacgattac cgattcgttg gttattccta
    13561 ctataggaat tggcgctggc ccaggaactg atgggcaagt ccttgtctgg cacgatatgt
    13621 taggtttaca gacttcattt aatcctaaat ttgtaaaaaa atattttaga gccaaagacc
    13681 attttattga agccttaaac agctatgtcc agcaagtaca acaaatgcat ttcccagcta
    13741 atgaacattc tttctaggaa aattatccat gcaaattttt cataatttaa atgagtggat
    13801 tcgctttaga aattccctgt ctcctgattt atccctgggg tttgctccaa ctatgggtaa
    13861 cttgcatgct ggacatgctt ctttgttttt agccagcagc aaagaaaatc actatactgt
    13921 ctccagtttg tttgttaacc cgacccaatt taataaccct gatgattaca agcattatcc
    13981 acgcacagtc gatgctgatc tcgaattaat gactcagaat ggagttgatt tttgtatact
    14041 ccccaatgaa aatgaaatct ataccgatgg ctatgcttat caagttcaag aaaaccggct
    14101 tgggcaattg atggagggaa aataccgacc ggggcatttt aacggagtcc tgaccatcgt
    14161 catgaaactc tttaatttgg ttaaaccaac ccgtgcctat tttggtgaaa aagattacca
    14221 acaactgttg ctaattcaag gaatggtaaa agcattgttc atggatattg aaatcaaatc
    14281 ttgccccaca gtaagagaaa aaagtggcct ggcctgcagc tcacgcaata acaggcttac
    14341 tccaagccaa agagaaatcg ctgatgaatt tgcaaaaata tttcatcaaa acaaatcctc
    14401 tgccatgata agcaaagaat tggaagcgct tggaattact gtagaatata ttgaagaatt
    14461 ccagggcaga cgatttgctg cagtaaaaat tggcgatatc cgtctcattg ataattattt
    14521 gctatgattc aaaccaattg gtgcgtgata acaggtggac cttcctcagg gaaaaccact
    14581 ttaattaatc atttggcaga gttgggatac aaaactgcgc cagaaatagc cagattttat
    14641 atcaaccagc tgctgtctca tgataaaaaa actctacaag aagtaaaaca tgacattctt
    14701 tggttacaaa gaaaaattct caatatcatg ttaagacgag aaagaaacct gcctaagaac
    14761 gacttaattt tttttgaccg tggcaccccg gacagcatcg gttattttcg ttttcatcat
    14821 ttagatgagg ctcatgtggt cagagcttgt cggtatttac gctataaaaa aatttttttc
    14881 tgtcatccct tgcctgttgt ccctgatacc ataagagatg aggatgatat caccgcaaag
    14941 aaaattagcg attacattta taatgcctat gttggcctta actaccctgt catagaatta
    15001 ccagcaacct caataaaaga acgcctggaa attattttat cccatgttga tacctagaga
    15061 taaacctgtt ctaggtttta ttcaaagcga ttgccgctgc aataaccgct gccttgtgtt
    15121 gaggcaagga tttatttgaa tactttaacc attcggggtg tgcctccact aaaccggtat
    15181 tgaatacccc tttctgaaac tcctctgatt ttatcatttc taaataataa ggaatggtag
    15241 tttttactcc aaacatgcgc atttcctcaa gagctcgacg cgcccgtcga agtaccgagg
    15301 gccaatcaaa agcccatacg gtcaatttgg cacacaaaga atcataatcc gggggaatct
    15361 ggtaaccagt ataaattgcg ctatctgtcc tcacaccggg tcctcccggg gcgtaatatc
    15421 gggtaatccg cccaaaactg ggtaaaaaat cgttttgagg gtcttctgca ttaatgcgaa
    15481 attcgatggc aaaaccattg tatttaattt gttcttgaga gatagttaga cgctcaccag
    15541 ctgcaattcg aatttgctcc tggactaaat caataccagt aatctgttct gtaattggat
    15601 gttcaacttg taaacgagtg ttcatttcca tgaagtaagg tcgattattt ttatccaaaa
    15661 taaattcaac agtacccgca ttggtatacc ctacctcctt ggctgccttg actgcgtatt
    15721 ccaaaagcac ttgacgtgtc gcctcatcca attgggggga aggggctatt tcaaccagtt
    15781 tctgatgtcg cctttgtatt gaacaatccc gttcaaacaa atgaagtact gttccatgat
    15841 gatctgccag gatttgtact tcgatatggc gtggattcgc tatgtatttt tccatgtaga
    15901 tatgggagtc tccaaatgat ttttctgctt cagattgcac gcgagcaaac tgttgcttta
    15961 tctgactggc atcatgacat aatctgatac ctctcccccc ccctccaaaa gtcgctttga
    16021 gcattacagg ataccctaga gtttctgcgc aatgtaaagc gtcatctata gtttccaaat
    16081 tcccctcgct tcctggtatg gtgggaaccc ccgcttttat catcgcttct cttgctgcaa
    16141 tcttggagcc catagtggct atcactggag cagacggtcc tataaaaacg atacccgcct
    16201 tattacaatc ctcagcaaac tcggcatttt cagataaaaa accatacccg gggtgaattg
    16261 cttcacaacc ggccaccttg gcacactcaa taatacgccg tccgttaaga taagcatcta
    16321 aaggtttttt actaagacag tgagaatgag aagctctttt gacatgcagg gcaaatctgt
    16381 ccacttcact gtgaatagta actgctgcta tccccatctc cttgcaagca cgtactattc
    16441 gcagggctat ctcaccgcga ttagccacca gaattttttt aaacattaaa aatctcttgt
    16501 aaaaactggc tcattgcttg ccatgaacga cgtgcggcaa cctcatcgta aattaatcct
    16561 aaatgttcat catgcgcttg aggattggta aaagcatgtt tcacctgccc atacatatgc
    16621 atttgccaat cgacatttgc ttctgtcatt tcctgacaaa aagcatgtac ttgctctggt
    16681 ttcgccatag gatcatcata accgtgcaaa gccagaattt ttgctttaat tcttccggct
    16741 gctagattct caggtttatt aagcaagcca tgaaaactta ctgctcctcg gatatccgcg
    16801 ccactcctgg ctaaatcaag ggcacacaaa ccaccaaagc aaaaccctat aatggctatg
    16861 cgattgttat cgactaccga tattgattta atggcttcca atgcggcata caaccgttcc
    16921 ctaagcaaag agcgatcgct gtacaagggt tgcattaatg ccattttttc atcattgtta
    16981 gaaccaattt gtccttgccc atacatatcc aaagcaaaac ctatatagcc catctcagca
    17041 agttgttttg ctttaaggca agcaaactct cctcttccac tccaatcatg agcgactatt
    17101 acggcaggct gaggttgttt aattccctct tcatgcgcta aaaatccgtg taactcaaga
    17161 tcatcatgtt gataaatata attcgatgaa tacataaaat atctcctaaa ttattttaaa
    17221 atagtctagg gcaatatccg taaaagtcca gtaattacat tagtttagcc taatgctatt
    17281 ctttgtcttt tgttcctcga ggctaagtaa aaattgtgtc gtccaataca aggatatcga
    17341 attgaattaa tctaatgctc ttgcatgttt caccaaaaca tgagtccatg agtcatgtaa
    17401 ataccaatat gtccactaat taactcaaat tatggatttg gtctaaaata aaaataaatt
    17461 atgtgaggaa tgttgccatg atgactgatt cgttaataca cataagggat ttgttacatg
    17521 ctcaattttg ctaccaaatg tttattaccc ccattcatct tcctatcgat aaaaaataca
    17581 gggaatttgc caaaatggcc tgtgaattca tagaaggaag acgggatgaa atcatacaca
    17641 gagaatatcc tcgccatcat gttttacatc gatttactcc cgcgaacaaa ccaaatggaa
    17701 aaaaaatatt gattacgcat ggctggatgt ctcgtgctgc ttatatggtt cgcttaatca
    17761 ggcttcttca taaagaagga tatgaagtct atgctatcga ttttcctgcc catggcgaag
    17821 ctaaaggaat tcagcttccc tggacagatg ccattgctat tatcaaagaa acgattaacc
    17881 aatttgggcc tttttatgga ttggttggcc attcttttgg tggttcaatg attttaaata
    17941 ctttgaatct ggcaggccaa ctaccagaat ggcaattaaa tcacaagccg gaaagagcca
    18001 ttttaattgc atcaccaaca caaatgcgaa ctcctgtcaa taaaattgcc agacgtttta
    18061 aattaagtgg gcatgcctat ttacaacttc gccaattaat ccgtcaacaa gcatcggtag
    18121 atcctgaaag gattcgttta agtcatttta tttcccaagc ccctgatacc tattttctat
    18181 gtatccatgg agaattggat gccactatca accccaaaga atcgataaat ttttgcaaat
    18241 attacaaaaa tgcccgcctg tctcttttac cagacgctga tcatgtgagt gtgttaatgg
    18301 atgagcgtgt cgaacaaatc gctttggatt ttttaagtct ttaacagggc agggtgtatt
    18361 gatagacctg gattactatt taacctaatc caagtcagta agcctacaat aagactattg
    18421 tgtctgctga ataaagaacc tcacatgcag aggaatttgc ataaaaatta tgcgatcaag
    18481 cttccatgta ttgacggatt ttcttcatgg catttttttc aagctgcctc acccgttcag
    18541 cggagacacc atatttttct gccaaatcat gtaacgtgag tttttcttcg gcaagccagc
    18601 gttgttgtaa aatatcctgg ctacgttcat ccagttgttc catagcaaac agtaattttt
    18661 cacgcccctg atcgccagta tcctcattct ctaacaagac agcaggatca tcgttcacat
    18721 taaacaaata acgctcaggc gctttgtaag catcatcatt atcatcaaca tctggcgcat
    18781 cataagatga atccataaca tttaaacgtt gctccatcaa caacacgtct tctcggctaa
    18841 cgcccaaatc tttggcgaca gcatcaactt cttcattgct gaaccaaccc aatcgatttt
    18901 tcatttggcg caaattaaaa aataatttac gttgggcctt ggttgtagca actttaacta
    18961 ttcgccagtt acgcagcaca aactcatgaa tttctgcttt aatccaatga accgcaaaag
    19021 aaacaaggcg tacgcccatc ttgggatcaa aacgttttac ggctttcatc aagcctacat
    19081 taccttcctg aattaaatca cttaaaggta atccataccc cagataaccg cgggctacac
    19141 gaactacata gcgcaaatgc gcaagaacta attgtcgagc agcttctata tctccttcag
    19201 aatgaaaccg ctcggcatat gcaatttcct cttccaaagt cagcattgga atttgattta
    19261 ctcgatggat ataagaatca agactaccaa caggtagatt cattgcagca agttgcaact
    19321 gttgactcat actacttcct ccggtcatta acactaaaat agtattaaaa tttatatcct
    19381 attaactgat aggattcacc aaaatgaatg ctaagctata aattttcaaa aactgcaaat
    19441 taagtaaaac ttcataactt agcactctct tcatgatgtt tcaattttgg tcattatttc
    19501 agcgatcctt ggcatattta taaatcgcta aacctcttat ataaaagttt tttacggttt
    19561 ataagcatat accgaatctg gacaaatatt gaccaaaacc aatgcttcat caactaatat
    19621 tatacattat ttgtagggtt ctatagaagc caattgccgc ttaacagaca acaaggctcc
    19681 aagccacccc agtataatag caaataacac aagtagcaat atttgccgta gtgacaaaca
    19741 agctaaagga tagtgcattt gatagacatt agccagctga ttcactgcga cgcccagggt
    19801 tagaataaaa atattaacca gaaagatggc taaaagtgcg ccagctgccc cataccaaac
    19861 tccagaatat aaaaaaggac gaataatata aggatctgtt gcgccaatca gttttaaaat
    19921 tttaatttct tcttgtctat tatgaatgtc cagacgtaag gtattgccaa taataaatac
    19981 tacagccaaa gctaataacg ccattaaagc atttgctgcc ttgccagaaa aacccaagat
    20041 agcgtgcaat cgattaatcc actccatatc cagtttcgcc aacgcgactt gtggaaaagc
    20101 ctgaagtttt cgagataata aatctaattt tgcaggagaa tctataacta atgcgggaac
    20161 tacttcaata actgcgggca aaggattttc aggaagatac cgcataatgt cttgcatacc
    20221 ttcttgttga gttaactcgg ataatccatc tgctgcagat ttgatatttg cctgaccaac
    20281 tccttcagta gtgcgtactt tttcaagtaa cgatgtttgc tccgcttccg gcaatgaagg
    20341 tttcaaaaac agggaaatgt gcccccctcg ctgccaacga ttggttagtt cacttaaatt
    20401 gtcagtgaat acccaaaaaa gtgtaggtaa cgctaaagcg atggcaataa caatgacggt
    20461 catcatggtt gctatcggtt tgcggcagaa aagattcaag ctattggccg cggcttgcaa
    20521 atgataggca agaagagatt gtgatcgttt taacacagcc ttcctccttt cagcatgacg
    20581 atgcgatgtt tcattccggc tattaaagcc aaatcatggg ttgcaattaa tatactaact
    20641 ccaacctgat taaattgttc aaaaatcttc attatctcag aagaaagttt tggatcaagg
    20701 ttaccggtag gttcatctgc caataacaat gcaggcttat gaactacagc acgagcaata
    20761 ccgactcgtt gctgttcacc aacagacagg tgaaccggta acattttctc tttactcaac
    20821 aatccgacca tatccaaagc ggcatgcacc cttttggcga tcataggata agcaacccct
    20881 tgaatttgca agggcaaggc aacattatca aaaacagttc tgtcattcag tagctgagga
    20941 gattgaaatg taatgcctaa ctgactgcgg tgagcagcaa catcccgctt tttgagatga
    21001 tttaatttca aaccattgac tgttaattga ccagacgtcg gccattcaag aagagcaatc
    21061 aacttgagca aggtgctttt gccagcacct gaatggccag taagaaagac catttctcct
    21121 ttttgcaaag aaaaattaac ttggcttaat gcctcaaagc cccccggata acgtttacta
    21181 acctggtcaa atgtgatcat catcgaatat cgcactaaca aattgcgctg cgtcaaaggg
    21241 cctcaaatct tcaatgccct cccctacccc taaataacga aaaggtatcc ccaaatcatt
    21301 cgctatagca aataatatcc cgcctttggc tgtcccatca agcttggtca tagtaattcc
    21361 agttaattgt actgcttcat gaaactgctt tgcctgagcc aaggcgttct gcccaatact
    21421 cgcatccaat accaacatgg tttcatgagg tgccttggga tcgagtttct gcatgaccct
    21481 tttcactttt ttcaactctt ccattaagtt gctttgagta tgcaaacgcc cggctgtatc
    21541 agcgattaaa acatctattt ttctcgcttt ggcagcctga agagcatcaa aaattactga
    21601 ggcactgtca gcgccggtat gctgggcaat aacaggtatg ccatttcttt cgccccatac
    21661 atgcaattgt tccaccgcag ccgcccgaaa cgtatcgccc gcagctaaca tgactttttt
    21721 tccctgcttt tggaattgtt tggctagttt accaatcgta gttgttttac cggctccatt
    21781 gacaccaacc attaatatga caaagggaga gccgtcttca gtttctatgg aaagcggttt
    21841 ctcttcctga ctcaaaatcg tttgcaaatt tgtctttaaa gtctgataaa ctgcgtcacc
    21901 attagacagt tgctttcttg ctaagccttc atttagctgt ttcaatacgt cttgagtggt
    21961 gtccaaacct aaatcagcac taattaataa ggtctccagc tcttcgagta actcctggct
    22021 gatttctttt ttacctaaca gcagccgact tatgccttcc cccaattgat gacgggtttt
    22081 gctaaggcct ttcctaaacc gggcaaaaat actttctttg ggcgaaggct ctttggcagt
    22141 cagttcctgg ggttcttcaa gcctggattc catgctgctt tcggcatgat cttgctgctc
    22201 ttcggtatcc aaatttggtt cggaattttg atttcgttta aaccatttaa tcattttagt
    22261 gtacaattcc ttaaaaatat gaaattctat catcttttta gctttgaggt gacattgtgc
    22321 acaaattact catttctctt atgatgctac tctcatttca ggcttttagc caagtccagg
    22381 agtatacgtt aaacaatggt ttaaaagtac tggtaaaaga agatcatcga gctccagtcg
    22441 tagtatccat gatttggtat aacgtgggtt cagcagatga gcctgtaggc atcacaggtg
    22501 tttctcatgc catagaacac atgatgttta aaggaacttc caaatacccg gtaggggtat
    22561 tttccaaaac aattgctgca ttgggaggtc aggaaaatgc ttttacaaac aatgattata
    22621 ccgcctatta tgaaaaatta gatgctgatc atctggctac aagcttcgaa ttggaagccg
    22681 atcgcatgaa taatttacag cttaatagcg aagaattcgc taaagaaatt aaagtcattc
    22741 aagaggaaag acgtttacgt actgacaata acccgcaggc acttgctttc gaacgttttc
    22801 tggccacagc gcatcttaca gcaccataca atcacccggt gataggatgg atgaatgatt
    22861 tgaaacaaat gaaagtagag gatttaaaaa aatggtacga aagttattac gcaccaaata
    22921 atgccacctt ggtagtagta ggcgatgtga atcctgagaa agtacatgcc ttggctgaac
    22981 gttatttcgg ttcaatagcc aaacgtccca tagcgtctcg taaaccccag caagaaccct
    23041 cagcgcttgg gaaaaaaatg gtttacatta atgcgccagc gaaattgcca ttactgttaa
    23101 ttggctatac tgtacctagt gtaaaaactg ctaaaaataa ctgggaacct tacgccttgg
    23161 aaattatcgc cgggatactt gatgccgggg aaagtgcgcg ctttgccaaa cacctggtgc
    23221 gaggcaatca agtagccact ggtgcagaag cctattataa cctctattca aggtaccaaa
    23281 gccaatttat tgtctatggg gcgcctagcc aagatcatca aataaaggat ctggaaaagg
    23341 cattaattac ggaattggaa gccttaaaaa aagcgcctgt tagcaatcag gaattacaaa
    23401 gggttaaaaa tcaaatcata gcacaaaaaa cttttgaaaa agattctatt tttggtcagg
    23461 ccatggagct gggattgtta gaaacaatag gtctgggttg gaaaaatacg gaaacataca
    23521 ccaaagccat caatgaaata acccccgagc aaattcagca agttgcgcaa cgatattttc
    23581 aagaaaataa catgactgtt gcagaattaa agccaatcag gcaagaagag gtaagaccat
    23641 gagtgtttta agagctttta tcatttcgtt tagtgtaatt ttttctcaca atctattcgc
    23701 caattcattc aaaactgaaa aatggcagac taaaaatggg gtgcgagttg tattttatca
    23761 agcaatggaa gtacctatgc ttgatattag tttggcattt gctgccggct ctgcctatga
    23821 cggtaagtat tttggcttaa gcgcattaac aaccaatttg attaatcaag gcaattccgg
    23881 taaagacgca acgactattg cggaagcttt agcagacacc ggagcgcaat ttaatgccga
    23941 aacgagtagg gatatggttg tccttagtct aagaactctt accagcaaag aggctttgca
    24001 gcaatcgacc aaaaccttta gccaaattat tagtcatcct gattttccta aaaaagcctt
    24061 cgcaagagaa aaagatcaac ttctcatggc agttgaacaa acagaagaat cgccagatga
    24121 tgtagccatc cagaactttt ttaaaacctt atatcaagag catccctatg cccatcctgt
    24181 gcatgggaca gttgagtcac taaatgccat taaagaaaat caagtcattg atttttataa
    24241 aaaatatttt gttgctaaaa atggtatatt agttatggtt ggtgctattg acagttctca
    24301 agctcatcaa ttggcagaac aattgaccca ggatctacct gcaggagaac cagcaccaac
    24361 aattcccaag gcctctcagt tggccgatgc ggaaaaaatt aatgtaccgt ttccttcctc
    24421 acaaacagta gttcgcttgg gacagatagg aatagatcat cataatcaaa attatttccc
    24481 tttaatggtt gggaattata ttttgggtgg aggaacatta gtttccaggc tgggaactga
    24541 ggtaagagaa aaaagaggct taacttacgg aatcgatagt caatttgtcc ctatgctagg
    24601 tgaaggaccg tttatcatta gtttatcaac caaaaactcc gaggccagaa acgcactgca
    24661 cattacccag gataccctga taaaatttat caaaaatggg cctaacaagg aggaactgac
    24721 ttcagcgaaa caatatctga caggtagttt tcctctctca ctaggaagca ataccaatat
    24781 tgcaaacctg ttattaagaa tggcgttcta ccatttacct gataattatc tggataccta
    24841 tgtcgctaaa atcaacgcgg taaccgatgc tgaaatcagg caagcattcc agcaacaggt
    24901 gaaccctgaa aaattactcc tggtaacggt tggacaatct tgaaacaggt aatacggatt
    24961 atcggaggat tatatcgggg gaaaaaaata catttccccg atgtagatgg cctgagacca
    25021 acaccagaca gagttcggga aacactcttt aattggctaa tgaatgatat tagaaatgcc
    25081 cgctgtctgg atgcttttgc aggtagtggc gcgttgggat ttgaagccta ttccagagga
    25141 gcgtctcatg tcgtttttat cgaacaatct cccaaagctc atgccagttt gcaaaatacc
    25201 attcgtcaat ttgatacccc caacttgcac cttataaaaa ctgatacgtt aaattatctt
    25261 aaacaaagca ccgagcaatt cgatctgatt tttttagacc ctccctacgc tctcaactat
    25321 gttcctcaat gtcttgatca tatcataaca aataaccttc ttgttaaagg aggtcttgtc
    25381 tacattgaat catcatctcc tattgaagta aaaacagaat tttggaagca attgaaatta
    25441 aaacaagccg gccaagtcat ttatggatta tttgaaaaac tctaacaaac aatgtcttga
    25501 caacaagcct tcctcctgtt tcaatcaaac taatatttcc aaatggtaaa gaacattgaa
    25561 tgaacaacaa taagattgtc ataatgttta ttttttcagc gcttcttgct ggctgtgctg
    25621 gaacaatgaa atttaaaaaa ccacccatca ataatccaag tgatgatgcg actattaaat
    25681 tagcagaagc tgccgtttca gtcagtgact ccatgcttga aatggcaaaa gttgaaaaag
    25741 taattactcc accaagcaaa gacaatacct taactatacc taatgcttat aacttgcaag
    25801 caagagccag tgtcgattgg tctggtccga ttgaagaatt gactgcacgt atcgccaaag
    25861 cagcacattt cagatttcgt gttttaggca agtcgccttc agttccagtt ttaatcagca
    25921 tcagtaccaa agacgagagt ctggccgaga tccttagaga tattgattat caagctggga
    25981 aaaaagccag tattcatgtg tatcctaaca gtcaggtcgt agaattgcgc tatgcgaaaa
    26041 tttattctta gtttatctat tttgctatca gccctcctgg tagcctgttc ttctcgtaat
    26101 cattacgggg atactggctc attggcaggc cttcaagcga tggctgattc aaaatacacc
    26161 cgagctcaaa aaaaacaaaa aatgggtaaa attcgcgaga tggcccttaa ggaaaccgct
    26221 ttaagtgttg gcgcccaggc tggcttagcc tggagagcaa aaataattga cgagcaatta
    26281 aataaacagg caagaaatct tgatgcaatt tatgatttta attccctggt cttagagcat
    26341 aatattttac ctcctgtatt acttgaggga aggaatactt tgaatcttgc agatgctcaa
    26401 agtatccgtg tttcagatcg cacctataaa gtcgcaaaac aagctcattt cattaccact
    26461 ccacctacct ggcggcaata tttgtggatg gattatgtga aacctgaagc tcccaatgtc
    26521 actctactcc ccaaaaccaa ggctgaaaaa gaaatatggt gcatctatac tgaaagaggg
    26581 tggaaaaatg gtatagatca ggcaaacacc attttggaag aaaacattgc ccgcattaaa
    26641 gaagatttcg gcggtatgat tctttataga aaattactgg ctatgaacat ggtatctcct
    26701 ccttatgttt ctcatactga cttgggcgta actggggatg gatcagaaat tcatatcgat
    26761 gacagggtct tgcgcatcac ggcgttacct gaactcaatg tcaatagtgc ggaatggaga
    26821 gctgcggtcg ccaaagatga aaatgctctc gaacgtttca aaaatatgga aaaactggca
    26881 aatcaggcta aaattgttat aacgaacaaa tcctggcaac ctattatagc accagtaagt
    26941 taatgtctat ggataatatt aatttaatgc ccgatgagcc aactcgtttc actccagtat
    27001 tcatggacag aatgctggag catgcagaaa gcctgaatgc ttcagatata acaatccaaa
    27061 ctggcgaacc catttttgca gaggtttatg gaagactcct taaaattaca aatcgacgtc
    27121 tttccaatac agaattgggc gaccttataa attccattta tggcccgaat gccacgacac
    27181 aattactttc cggtaaagat atagatactc attatgagtt tcggcccaac cgtggtgtac
    27241 gctatcgata tagggttaac gcgactgcct gcctggtaga aggtcatgat gccattcaga
    27301 tcacattaag aactattccg accacaccac ccaaactcag taccatgaac ctccctgata
    27361 acattattga agcgattgct ccacaggaag gaatcgtgtt tatcacaggc gctaccggtt
    27421 cgggcaaatc gacattgttg gcttccatta ttcgagagct tattgaaaca tcagactcaa
    27481 accgcaaagt attaacttat gaatccccaa tagaattcgt ctatgatgaa attgaaacaa
    27541 tttccgctgt agtaagccaa tcagagattc caagacactt acccaacttt gctgatggag
    27601 taagaaacgc attaagacga aagccgagat taattatggt gggtgaatgt cgtgatgcag
    27661 aaaccatcag cgccgctctt gaagctgctt taacaggaca cccggtctac acaaccctgc
    27721 atacttctgg tgttgcagaa acgatgcgac gtctggtcac gtcttttagc ggggaagagc
    27781 gtttgggaag aaccatcgat attcttgaaa caattaggct ttgcatttgg caaaaactgg
    27841 tcccaaccgt tgatgaaaga cgtgtagccc taagggagta tctggtattc gatgaagaag
    27901 tgagagatat tctattggag ggcgacccta acgaagtgac ttcagccact cgtaagttag
    27961 tgaggcaaaa agggcagtta atgacttggg acgcaaaaat gaaatttgaa caggggatta
    28021 tcagtgagcg agtttataaa ttaatcattg ctggggccaa agaatatcaa caataatcaa
    28081 aattaatctg cttgcacttg ccatagaaaa tcattgatat accacatata aaacaatcaa
    28141 gatcaaggat tcgattcatg atgtttcaca ggaaatgcct atactatttt tttaatttat
    28201 ttatttcgtt aattttaggt tcttctctca attgggcttc catcaatcag cacagcacag
    28261 attcgccaac cctcgacatt aaaattttag gcattaatga ttttcatggg caaataagca
    28321 gtgggagagc cgtaaaaaac gaaccggttg gcggggcagc tgtcctggcc gcttatctta
    28381 aagaagctca attgggaatg gaagatcgat caataattac catcatgggt gatttggttg
    28441 gagcctcccc cccttcttca ggactgttga atgatgaacc cagcatttta ttcattaatg
    28501 gcttgggaaa taatcaatgc aaacccgaat caagaatgga tcctctttgc aacatcgttg
    28561 ccacggtagg aaaccatgag tttgacaagg ggcaaaaggc catgtttgat ttaatttatg
    28621 gtaccaataa tccaccgact gattcatgga tctcgttacc caattatccg ggtgcatctt
    28681 atccctatgt ttctgcgaat atcgttgacg ccaagtcaga acaacctctc ttcccaccct
    28741 acataattaa acggatacat aatactccca tagcatttat tggcgctgtt ttaaaagatg
    28801 cggcagactc catgtttcca gcaaacgcag aaggaattaa atttttagat gaagcagaca
    28861 gtattaatcg ttacatcccg gaaattaaag cgaaaggagt caatgttatc attgtcctga
    28921 ttcatgaagg tgggaatcaa attccttatg agggagacac ccaaactgcc tcagcagtag
    28981 aaggtcatat taaagaaata gtttattccc ttggtgatga aatcgacgtt gttatgggtg
    29041 gtcacaccca tcaatttcta aatgctttcc ttccaaatcg caatgggcat aagatcctgg
    29101 tcacacaagc gaatagttac agtgctgctt ttgctgaagt tacccttcaa atcgatacca
    29161 aaaatcatgc agtaaaaaac aaatcagcca aaattattac aacttatgct aatcgatggc
    29221 ctggaacaaa tccggataca caagccttgg aaattgttaa attggcagaa gataaaattg
    29281 ggccaataat aaatagccat gtaggcactg cagggtatga tctgctaaga aaacaaaata
    29341 aagatggtga atcgagttta gggaatctga tcgcagatgc ttatagaact gtaatggata
    29401 ccgatctggg tatcactaat cccaatggaa ttcgtgatga tttaaaagca ggggaagtta
    29461 actggggaaa tatctattct attttaccct tttccaacag gatagttata attaccttaa
    29521 ctggaaatga tatttatgac ttgttagaac aacaatggat gggctcttat atcaatatgt
    29581 tacaaatttc cgggtttgcc tacacctatg attcacatca acctctggga cataaaataa
    29641 ttgaaattag acatcaaaac aaaccattgc ttagagaaaa gatgtatgcc atagccactt
    29701 cagattttct tgccagcggg aatggagttt tttcagttat gaaaagagga aaaataatcc
    29761 atattggtca aagtgatcat gacacggtaa tcgcatatat taaaagttta cctcaaccct
    29821 ttcacgcgtc cattgaggca aggataaaag aatcagagac tggattaaaa acaggagttg
    29881 gatagattaa attcgtttta ccgtcagccc ctgtttttat tatttattaa ttaagttttg
    29941 ctattatttt atgcattcta atccagcaca tagtagaatt tgttttcgct aagtcctcaa
    30001 gttcattatg taattgtttg atttcctccc ttttagctat accaagctct aaaagctgag
    30061 gggtcaaatc atcaagcgcc attgaataaa ttagtttttc tttgttcgtt ctgagtaagg
    30121 gttgaaataa agaacaatgc tcaacttcaa aaccgcagga tgaaaactct tgctctaatc
    30181 gatgtgctat ctgaaaatcc cgattgtttt tgctaaagca tgacctcgcc cattgtgtaa
    30241 actggtcata ggagggtgaa ggaggagagc aaaaggtaga atcaaataaa gagggttctt
    30301 cacatacgat gaaaccacca gaattaagac aatttttcat ttgaagaatg acagagcgcg
    30361 catcagccac atgatgtaaa accattcgac aataaatcaa atcaaatttt tccccaagat
    30421 ctgaaagatg attaacatcc cacaccttaa atcgtatatt gctatagttt ctggttcgtt
    30481 gttgagtatg atccagttga tcctgactca agtctatagt aagcaaactg ccttcccgac
    30541 caattaattt ggctaattcg agggtcatat caccagagcc acaccctatc tccagagcag
    30601 tcattgccgc tgaaggccca acctcagaaa gtaaatccag gctgctcttg gcatacaatt
    30661 gatgttgcag agaaagacgc tctctcgcct tgtcattggt atttagaatg taagtgctgg
    30721 gcataatata tccttatatt tgataaaaaa taattattgt tcttcaaaaa gagttacatg
    30781 gctttccctt tgccacaaat gctcatttaa atattggttt aaatgttttg cctgtaattg
    30841 aaacatctgg tttaaatttt ctttagttga gctacccata tgtggtgata agacgacgtt
    30901 atcaagttgc cttaaagcga aaggaacttc cggttcatac tggtatacgt ccaaagcggc
    30961 accagcgatt gtatggcgtt gcaaagcatc aatcagagcg ttttgatcca ctatactgcc
    31021 tcgcgcaaca ttaatcaaat atccctctgt ccctaaatta tctaaaacct gtttgttaat
    31081 taaatgttgt gtatcaatac cgccggaaca acaaatgatt aaaaaatcac taatagatgc
    31141 taaatttgcg gctgttggac aatacaagta agggctattt ttttgacttc gggccgtata
    31201 agcaattttt agcccgaaag gctctgcaaa ttgcgctatc ttttcaccaa tttgaccgaa
    31261 tccaaccaag cccaattgct ttcccaataa atggttgccc agaaaacggg gctttttctc
    31321 aacccattca ttatttcggg tatagcgatc atttagaatc acccttcgcg acagtgctaa
    31381 caataaagcg atagcgagtt ctgctgtatc atgaacacct gcattaggtt gtgaatgcag
    31441 aataatatga ttttgcttta aaaaattaat atcaatattg tccgttccta ttcccaggtg
    31501 actaataatt tttaagttag gaaattgcat taaaaacgaa tggtcaatct gatcccatac
    31561 cgttgtggct aaagccacaa cccgggattg atccgctcct gaagccattt tccaaccatg
    31621 aataatgttc caatcaggaa tcagcatggg aataattggc tctagaaatt ggttggtcag
    31681 gtaaacaaat ggcttcatta aaatgtcctg tttttcagaa taaagattcc gtgtgttgcc
    31741 catgatattc tttctcatct ttctgacaag caaataaaat caagcggtaa aatgagcgtt
    31801 ctaaagtaga gacatatagc gattattacg gttatgatac tatattcttt tactattgca
    31861 tttcattatt ccaaggagac gttatcatag agaggaatgc acagcaatat tttgaaagcg
    31921 cgaaggaatt gttcttgcca gtaatacctg tggaggagtt tgatggtttt atacttaaat
    31981 tgggcaagaa taactaccat ttttgcaaaa atgaaactcc ttttaataat agctgtagtg
    32041 ccaatattgc tattgataaa tattgtacct ctaaactatt ggaaaaagcg ggtattcctg
    32101 ttcccaaagc tatctcttta aactgtagtg aatttcaacg gaatttgcat aaagataaaa
    32161 ttgcacaatt aaactttccc ttagtcatta aaccaataga cggttcgctg ggaatcggcg
    32221 ttttatgcaa tataaaaaca tgggaagaac tggaaagcca tttaacaaaa tatttttcct
    32281 cttatcagtg cttaataata gaagaattcc acggcaaact gaattcttac cgtgtattgg
    32341 tttttaataa ccgtatcctt ggtatagttc aacgttaccc agccgctgta actggcgata
    32401 gagtacatcc catacatgag ttgattaaac aagaaaattt aaacagaaaa agaatcaatg
    32461 aggctttagg tgaaattact ttagatgagg aagctcggat taaacttcaa gagctgggca
    32521 taacagaaaa ttacatcccc tcttttggag agcaaattgt tttatgctat acaagcaatg
    32581 caacaagagg tggatcctat gtcaccataa acaagaagat gtgcaagcaa aatcgtaaat
    32641 tattgcttca agcagccaaa atactcgact tgcaattagt tgggatagat gtggaatgtt
    32701 cggatatcat caatattcct atagaacaat caaatggcgt catactggaa gtgaatcata
    32761 agcccagtat tcgaattcat gagtttccaa tgaaaggaac cccacaaaaa gtcacaaaaa
    32821 aaattatgcg cagtttcatc acccgccatc cgttgtctta cttatattct atctacaaca
    32881 accaaccgac cgctttttat gtccgcagct ctattcttat tatcattatg ggattaattc
    32941 ttttagccgt gtggtaattc acctcagcta aaacttatgt catttcccaa tctttacttc
    33001 caatacctgg cagagagctt atttgataat aaatttattt ctgaaatcac tttgctcagg
    33061 taaaggtagt tcacttcatg ataaatgctg ataattcact aacctcgatg aaagaaattt
    33121 gagattaatg tacttaatca aacaatctat tgagcaggct cattttgcaa gtgctctttt
    33181 aaagtttata cttaataata atgaggttaa attatattac gaggtattaa atgaatcctg
    33241 tatcgatttt agatgcctca ttaagagatg gtggacatag aactaatttt cattttaagg
    33301 atgatgattt aaaacaaata ctacttcctc ttgataattc gggtattgaa tacattgaaa
    33361 ttggctaccg taatggttca cttcacccta ttgaaaatat agggaaagct ggaatgtgtc
    33421 cccaagatta tctcctgtat tgccaatctc taataaaaaa agcaaaaatt gcagtgatgg
    33481 ttcatcctca taatgtaaac gaacaagatc tgctcgagct aaaaaattgc ggtgtgaaat
    33541 taataagaat atgtatagca aaaggtgatc tggtaaacgc tgtccctctg attaaactgg
    33601 ctaagaattt gaattttgaa acctcagtaa acattatcca tatttcttat tatacggaaa
    33661 gcgaactgga tagtttgatt gaggaagtaa gcaaacagga accggatatg atctattttg
    33721 ctgattctaa tggaagctta tttcctgaca ggataaatag cctgtataaa aaatacaccc
    33781 gccaatataa agtttctttt ggatttcatg ctcatgacaa ccttggattg gcgcaagcta
    33841 acgctcttgc ggcggtaaac gccggggttc attttattga tgcctcactg gcaggaatgg
    33901 gcaagggaac aggtaatcta aaaactgaat tttttatcgc ttatttacat gcgaacaata
    33961 taaaaaaata taatttggaa gatgtgttga cagcagctaa ttatgtaaga gatgctttga
    34021 aaataggaca agaacccatt gaaatgagtg aattcataag gggaatttct gatctgtcta
    34081 cagcagacct gaaaagctat aaaccaggca agctctagaa aatgagtaca tttccaatat
    34141 aggaatataa ggttggaata tcttgattcc ccaagatttg atgttccaga aagcaacgta
    34201 ccttcaaaat taagctggca aaatcataga tcatccagga ataagtagag aatacaatgg
    34261 caaaaaaaag attattgatt atcggccctg tttcaactca atcctctagt ctgaggcaat
    34321 taagccaaaa tttatctttt cttaatcctt attactccat tgagtgtctt gatcctcttg
    34381 tcacgttgca agagtgtccc gatgaggaat attatggaat ttggcaaaat caactaaagc
    34441 cgctcatcaa aatttatgat gcatttgttg gcttttcttt tggtggtgtt attcttcaac
    34501 aatgtttttc catattgagc ggggaaaaaa aacaaatcat tcttttttcc acaccaacat
    34561 ttgcagattc aatcttaaga gaaaaacttg aaacagtcat ctccctctgt tctcaaaata
    34621 agctggaaga aggattgtct tcgctctatc aagcagttta cgcgcctcat tctattcctg
    34681 atgcttcact gcagtattta aatagggaac ttgcgatcaa gaggcttatt tttggcttaa
    34741 ccaaagtatt aaaaacaaat tgtgtttcaa ttttaaatca ttgtaatgtg aatcaccttc
    34801 atttaatagg tgaaaaatca caactggtta ataccaagaa cgtgataaaa ccatcatgtg
    34861 gccaattgat tgtagttccc gaagcaagta tgcgagttct ggaaagcaac cccacttttt
    34921 gcaaagaaat tatatttaag gccctgaatc atgaaatcca ctgatcctat aaaaattgct
    34981 gtattaccag gtgatgggat tgggattgaa gtgaccgagg ctacgcttcc tgtttttgaa
    35041 gtccttgatg tccctgcaac cctgagttat ggcgatattg gttgggaatt ttggaaaaaa
    35101 gaaggaacag caatcccctc cagaacatgg caattgatag ccgcctctga taccgttttg
    35161 ctaggagcga ttaccagcaa gccccaacgt gaagccaaac aggaattaag caaagcgctt
    35221 agaaagagca acccttatta cgtatctcct gtcattcaat taagacaagg tttggatctt
    35281 tttgcaaatg ttcgcccctg tttttccatc gatgaccaat caaagccgtt taatttttgc
    35341 atcattaggg aaaatagcga aggattatat tgcggatttg attattttcc attaccaaaa
    35401 gcaatacata atttattagc agaaagccaa cattggcaaa cgattccagc tgatgaagca
    35461 agttgtgcct taagattgca atcaaaaccc ggcttaaccc gtttatttga ttttgccttt
    35521 aatcatgcca tgcaaactgg catgcctaga gtaactttgg cagataaacc aaatgtgctt
    35581 agagaaagtg gcgaatttgc caggaaaatt tttgaaagca cagcccaaaa atacccgaaa
    35641 atccaagcgg acatactcaa tgttgatgcc gtagcattat ggttgataaa aagtccggaa
    35701 aaatttggag taatcgtcgc ggagaatatg tttggtgata ttttatccga catcggagca
    35761 ggcgttatgg gaggattagg acttgcaccc agtgccaata ttggtgataa agggagttac
    35821 tttgagccag tacatggtag cggaccaaga atgaaaaaaa attgtgccaa tccatcagct
    35881 atgtttttaa caattagcat gctgctaaat cattttggtt atcctgatcg agcaaaaaaa
    35941 atagtcaatg ccgtgatgca agtcataaag gaaaagcgtt ttattactta tgatctgggt
    36001 gggcactcca ctacaatcga tatggcgaat gcagtaattg aacgttgtgc gcgattagaa
    36061 gcttcttgtt tatcaacaga ttttagttcg gtccctaaag aaaatttaat tgagtcagat
    36121 actatgagca acttgcttca gcaattaatg cactgtaatt ctgcagaaat ttctgacgcc
    36181 ctggacgctt gtggaatcga aggcggatta ttgaatatca aacccttatc acagggaatg
    36241 aaaatggttg ggcctgctta tactatccaa tatttaccca gagaaaaaaa agaaacagcc
    36301 ttccataatg ccgcaaacta tattgacaaa gttcccaaac attcggtcat agtcattgac
    36361 aacaacggtc aaactgattg caccgtttgg ggtgacctgt taacacatac tgccttgaga
    36421 aataacatca tgggcacggt tgtgcacggg gccgtgagag acgtcgaatc aattcgctcg
    36481 acaaactatc ctgtgttttg cacaggaatt tatatgtgct ctggtaagaa tcgcgtttac
    36541 aaagcgaatg agcaatgtcc tttattcatt aatggagtta caataaatcc aggcgacatt
    36601 gtctttgctg atgacaatgg agtgctggtg atcccaaatg agcttcttca cgaggtagtg
    36661 aataaaacaa ttgccataag attaactgag gaacgcatta aagcagctgt agcatcaggt
    36721 tccactcttg agcaagctcg agaagattat tactatgaac aaccctggtt aggtattaat
    36781 aaaaagagag agcaatagtg tttttgcctg aaaaactcaa attcatagac atacactacc
    36841 atgcagcccc tgatctttat attcgtcgca gaaatgcaat tgaagcggga agattgtatc
    36901 actctttgtg tggtgcagtt gttttaaaaa gtcatattgg cagtactggt attcaggcaa
    36961 ctattgccca agaaatgggt ttacccgttt ttccttctgt tgtgttaaat catatcaatg
    37021 gaggaataaa ttatagagta gttatccgtg ccttgaatga atatcaacct cttttttcgg
    37081 gtaaaatgct cgttgatttc ccaaccatca ccggtcgtaa actgcaatca aaactatcta
    37141 gggaattaac tcacaataat ttaaaacaat atactcgaca gggagaaaca ttgtttggat
    37201 caaaacaaca gctacgcaaa gaagtgatag atattttaaa aatggcccgg gattacccca
    37261 ttgttttaac ttcaggcctt gcctcgagag aggagattta taggctaatt gatgcctgca
    37321 atcaatatga tgttccttca ttattgttaa gccaaccgtc taatccttta accggtttaa
    37381 aatttactga actcaatgaa ttgattaaaa atgagtggtt atgggtggaa cagaccgcct
    37441 taacttttat ccttgagcac caggacaaac aagatttttc acatgtatta actcatatcc
    37501 ctcgagtcat ttacagctcg gatttagggc aaaccagcca aatggatatt ccagagtggc
    37561 tgcatttctc aaaaagaata tttaatgaat tgggtttatc ggcaaaacga atggaagagc
    37621 tatttctctc caatgccatc aaattactcg acttataaag tcccaataga tccttgcata
    37681 acctgtccct ttcattttat tgcagttaat ctctgcccct caaaggcttt gcagcttgcc
    37741 caaaaacgaa aaagtcaggt tatacaaagg tctaatatca ataaatcatg caaatagctc
    37801 ttgcaaaaac aattgagtgc tttgccagga acgttgatcc gctaattgat tgtaatgtag
    37861 tcccatttca tcatcattcg cattagggtc agtaaacgaa tgagctgttt ggccataaac
    37921 atgaacttgc cagtccacct tcctttctgt catttcaata gagaattgat taacttgctc
    37981 gattggtact aaagggtcat cataaccatg caatatcaat actttcgcat gtaatggctt
    38041 attactctca ccctccaaag gtaataatac cccatgaaaa ctgacaaccc ctctaagcgg
    38101 tgccccggaa cgtatcaaat ccaatacgca caacccaccg aaacaatagc caatagcagc
    38161 cactttatca ctatccacct caggcaactg aaccactttg ctgaaggccg cattaatacg
    38221 agtgattaac tttcctctat tttctctgaa aggagtcatt aaagctcttt tctctacctt
    38281 atccttgcct agcttggcat ttccatacat gtctatagca aatccaatat accccatgtc
    38341 agcaagctga atcgctttgt tacaagctcc ctctccacgc cccccccaat catgggcaac
    38401 cataacgcaa ggtaacggtt ttgagacact ggaatgatag gcaataaaac ccttgcacac
    38461 agtatcaccg tcctcgtatt ccacttcctt agtcactaac atagtaatcc tcttttttaa
    38521 aaggtcgtcc aaacagtatt aataactaac tgtcaaagtc ttaataaaat gaactgccca
    38581 taaataataa gaaaggctcc tttgagccct tcttatcgta ttttttagaa tgttattcag
    38641 gaggtggtgt actggttggg gggccactat ctgagccgcc cagttgttgg cctgtatccg
    38701 gtttagagct tggtccagtc tggctgccac tgtttttacc tttacctgaa acatcgcccg
    38761 cattttctga aaccttttta gcttgaggcc ctaatttgcc aagcatctcc tgccctttag
    38821 cgcccaattt gtcgccaata gctttctcag cgccataagt ggcctcgcct gctttctcta
    38881 ctttaccctt ggcttcttca ccccattgca tggtttcttg gccaaaagat tcagggctgc
    38941 ctcctatcca tctcagcact ttatctggca ggtgagcaat cagcgtgaag gccttttgca
    39001 cgataattaa atacatggtg gtataaatta agatggagaa gaagaaagca tacactcctg
    39061 cccagcccga ataaccgccc tgcaatgcag ttgactgata atcaccgctc aatttggatc
    39121 caaaatcatc ccattgcgat gtgtctattt tcgaccctgt ccaattcgcg accgtctgcc
    39181 ctgcctgtat cattttacag tcagcaggat acatttgaca gtatgcagaa ttagcatttt
    39241 tgtctgcgaa gtcacctttt ttccatgttt ctattccttg atccgattgg atgtaggaaa
    39301 tggcatgatc aaatccggca ttcaaaatcc agactccgac ataggacaag gcaattgctg
    39361 taatatatcc tattatcatc agagaaggtc ttaaaaagac attaaccaaa atcataatgg
    39421 caaattcgcc tttaccaaaa gcctcattac cttccggatg tgtaacaccc aaagcgacaa
    39481 ttggggcagc aaccatagcc tcgataacgg ctatcagcca ggcaaatgat ccaaaggtaa
    39541 aaatcatata aggtaaaacg ggtatataat atgctgtaac aaatcctatg gataccatgg
    39601 ttcctatcca tgccatcaac aggggcatag ccatcataat caaggcaaag ataaagatac
    39661 caaaaagtgg gattagtgaa ctaaccacag ccatattcaa cagtgttaac cacaaagtac
    39721 cagaaaaatt aatatacatc gtccccatat tagccaacgc gactatgggg tttactccag
    39781 gttgcgtaag agtttgaact cccactatga aaatatcttt catgccttgt aaaggaatca
    39841 ttaaaaaggc catgactatg gaattaatca tttccccgaa tatagctaga aagaaattgt
    39901 acacgtacct gaaaacatag ttataaaata aatcacccat catgcggcca aggcaaaaag
    39961 agaagaacaa aatcttcact ctgccgcaat caaaatcctg gtgcttcatg taataaaggg
    40021 aggtatctac tttaaaatta ataagattgg caaaggttaa cggttttatt cccggctggc
    40081 ccggcaattg aaccatcatg gaattgttga taaatccata gactgttgaa gacaatggtc
    40141 cttccacata tgcacgttta gggtcaggac ttagatcagg ttgcttgacc ccatcctgac
    40201 tcaaggctgg tgccccattg attaatgact ggacttgaac cagcttatcg ctcttgtttt
    40261 gaaaccagtt gcacaataaa gcatacgtgc cctgacacgc tttcccaaat ggcttggtga
    40321 gttgtgctgg atcaaaacta ctattatcaa ggcctgtgtc actatcaaaa tcactctcgt
    40381 cttttactgc acttccattg agtttgacca aatcaaaaaa ataagagcca gccattatcc
    40441 atcctttggt attggcttct tggataaaat cacgggattt gttatcgtat tcagaagatt
    40501 tagattgttt tagcaggtta atggttggcg tcataatgcc attgtagtca ttaatcgccc
    40561 ctaaaaattc agtaccatta aataataccc ccggagtaga tccggtagaa ctaggtatcg
    40621 atccccatgt ttggcaaact tgttgatact cagtacagac ctggccagat gccttataag
    40681 gtactccaaa ctgctgtttg gcaatggcag aaaaatcatt ttttgagttc ccggtagacg
    40741 aagttgatga aaaggccgga tcattattaa ccatcacctg tgccaccgtg gataaagtca
    40801 catacatttg ctgtatagca atcgcgcgag acaattgagc tgtttccaac tgggaagaag
    40861 taagctttat gttatttccc atatcagcct tcaaatcaga aatactattc catgttactg
    40921 tgccgcaaat cccatttaag aaataaaatg gtgagctttt atcgaagttt ggcatatcaa
    40981 ggctaaatgg tcctttagac ttgtcatcgc tttgcttctt gacaaaattg actgtactga
    41041 tgaagtcagg aatcgctgta cggcaaaaag tattcatttc aggagtagga ttacccccac
    41101 aaggagggct gttactttgg ctcagataaa gattcctttg cgtttgtaat tgtttttgta
    41161 aacccaacat gcagacttga ccagacaata tagtaaatgc cccttttgct atacctgtag
    41221 acttcccacc tttatctgtg aggctcttgg ttgggtcaga ctgagcctgt attatcacac
    41281 ccccccgatt cagataactc aaggcagctt cccatatttt atcagctgca ccaacacctt
    41341 gcacaataac ccacataaaa aatacctgca tcatacaata accagatgct ttaggtatta
    41401 acaaagccaa accgaaggtg gaacgaagag gaatccagat tgaggaccat ttttggccaa
    41461 gcatttgccc ttcatgggct gtgttcatgg tggaaaccat gagggtatac ataatgatga
    41521 ttccacccaa agcaaggaca gcggaattga agacaccaaa catattgccc attatctggc
    41581 ttcccgttcc gtgtagtacc ccatcgacta caccaaacag gttacctaaa aaaacgacgg
    41641 acaaatcgct tgctggaggc gcgaaactta aggagccact ttcagcgaga gcaagcgcag
    41701 gaaaaaagca aaggaggacc gtaatagcta atttattcat tctttctcac ccaataaccc
    41761 ttgcctgtac cattctttaa cggtacatcc caatttacgt tgtttaatct ggtagtacca
    41821 aaaatgataa cgaaaggcta gtaccaatgc gatcataacc acaaccaaac ttattattgt
    41881 agctttccat gaaccataaa aaagttgata ccccatgtaa atgagtagca taaacgcagc
    41941 aataaccata atcacactta gccggaacaa ggctttttgt ttgattatca agtcagcctc
    42001 attcaatttt agttttttga ctgcttcatc aaatgattcc acatgggtgg gttcctgtgg
    42061 gacgaataaa cgcttaacgc cattgaccaa atataaggtg aatgacttca ttctgtccca
    42121 atcgacccat ttacgaacat tgataatcct tgtaataact cttacgatcc ttgatcccga
    42181 tttctttttc atatttttta ctttttcaca attttaagta atggactttt atatatcata
    42241 atatactata gtattaagta gttgtttcgt ctatcaacgt atctcagtaa aaaaagagta
    42301 aataatgcct gatttaagcc atgaagcctc cgcaaagtac tggtttgagt accttgaccc
    42361 catgatttac agggtcatca cttttatgga aagtgtggaa aactggactc ttgatggaaa
    42421 tccggaactg gaagaggcaa tgaaacagtt agggcaagaa cttgatgata ttgaaaaaat
    42481 tgacttgggc ttgctagcag aggaagacaa atttattcgt atcgttggaa acatcaaatc
    42541 aggaagaggc ttacgcttgc ttcaggcaat agatacagtc catcctggta gcgcctcccg
    42601 tgttttaatc catgcggaag aaaccagttt aagcagcaat gacccggcag gtttttttct
    42661 aaaacgcaat attgtatttg aaagactaag gttattatcg cgagtttttt gccaataccg
    42721 tctaaaactc gttttacgag cactcgaagg ggatgaataa tgaaagtatt acctaaactg
    42781 gccttggcca atctgttttg ttttaccacc ttatctgttt tcgctcaagg acaaggatat
    42841 gatccatcgt accagcagac aagcaccaat acgtccaacc tggtaactta cctgaccaat
    42901 ctgggaaaat atttaggcta tgacatcact caatccaata aaacgcctaa cccgccttat
    42961 tcacaattat tcaattccaa tgtagcccaa cttattcaaa gttacgctta caataccttt
    43021 ttaggcgcta ttcctgtcaa tgccatgtca cagagcttaa tgaatttcgt caccaataat
    43081 gtgcaaggaa atacattaat aaataacttg gcaaacagca cctttaaata tcaaaatttc
    43141 agctccccat cctccggcgc ggatgggaaa attacagcta acggcataat agaccaatca
    43201 acaaccactg cccaagctcc cactcccacg gggatttttt cagctccaca agccccaact
    43261 gccggaactt atttgaacga tccggtcagt caggccgtat tcaatattct gggaaccccg
    43321 gattacagtt tttgtatgga caacgatcaa aaaaactgga aatccgattg taaactcaac
    43381 tatcaaaatc tggtcatgca aaatgttatc ggcactttgc ccccggcgct atctcaatcg
    43441 ggacctgttt tttatagcta tgaatacaat aaaccgatta ttagccaact aaacagcaat
    43501 tcactcattg ccccattact attggatacc tcttcgtcac aatctgggca aaactctgga
    43561 ttaacggcaa aaagccaagc ccagcaagcg cttaatttta ttagatacgc aagcgctcaa
    43621 gtcactcctc cctccttacc caagttgagt gcctacagcg agttatggaa tcaagccact
    43681 gccaaaccga attctgaagg ttataatgaa gtccagcaaa aacaagcggc ggcaacattg
    43741 agcggttatt ttaataattt gcgcgtttac gccgcacaaa cttctgtggg cgtaagtaac
    43801 ctctactata ttttatccaa acggctaccc caaaatatga gtggaaatca aaatagttcc
    43861 aatatcacca gccaggcttt gaatgaattt aacatggcaa ctcgacgatt atttgatcca
    43921 accgcctcca acattcccgg acaaccaaac caacaatgga ttaaacaaat taatgacgca
    43981 tcccctgcca cagttcaaaa agaaatagcg gtattgctgg ctgaaattaa ttatcaaatg
    44041 tatttggaca gacaaataca agaacggata ttgttaacca acagcattat gctattgcaa
    44101 aacttaaaag cagcccaacc gaccgcagat ttttcaaacc aaagctctcc ctctgagcaa
    44161 caatagtcac cactttggct cctgggattc caattccagg gggccattgt aacccgtttt
    44221 attaacacgc ttaaaaattt ggtacacaaa atcaatcatt tttttccctt gcggttggtt
    44281 tttgtttaaa taaaaccccg aatttaatgt gctaactaat tgatagtttt taaaaaaatg
    44341 tttttcatat agcaaatctg ttaaataatc accacgtgct tccatagggt agtaattggt
    44401 gatcacccaa tcaggcttca ctttagaatt gagataatcg gcataaagaa ttaaatcgtt
    44461 tttaaatggt ttttgggtta aatcgctatt gttcaaacat tgcgcgtcga taaatcgaat
    44521 gtcttcgcga gaaaaaaaag gaataatccc acaatcatct aacagaacgc tgtcgcctct
    44581 ttttgccttt cggttgagca aatcagcaat agtcattcta ttttgagtgc gttcattata
    44641 ttctgcaact gatttggata aatgatttaa atcattacca ggcacaaaaa accaggtaat
    44701 aaacaacatt aatacagcag ccagatattc aggatcccat ctgaaaaatt taaaatattg
    44761 tatcaattga ttaagcccta aaacaggtaa tatggaaaaa agggcaaatg ggcttaaaaa
    44821 caatcttaaa ccgtatgtga ctacaggatt tgctttccaa agcaacagag tatataaaac
    44881 agaaggcaac catagtaaaa catggcgaca atctttattg gcagacagta aatagggtag
    44941 cgataataca agcaacggtg ttataaaaag aaaataatca aaatctactg ccaaaaactg
    45001 cccataagct aacgctttgc aacgatagct attaggaatc caatggccga aataaattaa
    45061 tcgccaaata aaatacgcaa gatagggcaa tacaaagcaa ataaaaataa ttgaaaccca
    45121 taagaacaat ctcttatgat ctttaaaaac ctgctccctt tgagccctca aatattgaca
    45181 tcctataaaa acagccaaag gaattatcca aatgattccc tcaaatcgtg ttaagctcaa
    45241 taataacaag agcatattgg ttattatcca atgagaggtt gagtaaagag aaaatcgatt
    45301 gggccatggt ctgtcaccaa cagtttgata accgaatgcc aaggtacact gccatatcaa
    45361 tgccaaagac agagcgcagt aaaataaggt ttctaatcca ctgaccgccc accaagtcac
    45421 tcctgtataa tggctaaata aaaaaacagg taatatggat agtagagggc tacaaaaaag
    45481 tcttgccagt cgatataaaa acagtaatgc caatcccagg ctacaacagg aaatgacctt
    45541 catactgctc accagaggta attgcaattt aataaccaag gcagcaatca taagccataa
    45601 aaaattggaa tacccctcta cgggaggcag ggtcacatgc caaaacagcc ctttgccgct
    45661 cactaactgt cgtgcataat accagctgat ataagcatca tctgttgtga aatcccaagc
    45721 tgtatacaac gcgaccatca gatcgtaagc gcaataaatg cctatcaata gcaccagaat
    45781 ggtttttctt cttgataaca gggaactcaa aatgtgttct gctccagaaa atttttctat
    45841 aacacaatcc acaatagcaa tatcacaaag tatcaacaag ctccaaaatc atgatgcaaa
    45901 aaaaataatg ggtatgttat gattccaacc caattaaaaa aaaatgtatc ctcaatggaa
    45961 taatacggaa cttacaagaa acgccaaaag tctgggtgat tgtgataaga gagtaccaca
    46021 ataacatttc aaaacagtga atcacatcca ctggactaaa gatgatagac cttgatttaa
    46081 attaaaaatg taacccctac tcagtggttg gtgttgattg ataaatccgg tacaaagttc
    46141 tcaggatgag ttaaccattt aactcagcga gcgattatgt ccaaacctta ttatttttat
    46201 gtcagcggaa tgaattgcac cagttgcagt aattccatag agtccttttt aagaaattcc
    46261 aatttaatca aaatcaatac atttcatgcg gacatgagca ccccggatcc aaaaaaaatt
    46321 gtgatatctc tttctgaaga aaaggaaaat gaacatgaaa gcaactggaa aaccattaaa
    46381 aaattaattg atgatacagg ttttacttgc gaaaattatg aattccctca ggtgacagat
    46441 caaaaacctg ctcttcctct cgagaaaacg cagacaaccg ttcaacagga agccagccaa
    46501 ttatccgcac tctttagcaa cgcaaaaaaa ataatcacct cccattggtt gcttggagga
    46561 ttgggttgta tcactggggt tagcttaatg atcctgacta tggctctagg tagtttacca
    46621 cttgcattaa tgattctttt agcaagcatc agcactttat taactattgc gcttggtgcc
    46681 caatcttatt acgaggcttg gaaaaagtta gttcaatctc gcacactcac aatggataca
    46741 ttgttttcaa ttagcaccat ttctgttctt attgtgtcgc ttgcttcatt ctttgtaccg
    46801 tggttaccta tgatgtttga ggcaggatta cttatttatg gattcaggca tattggtatc
    46861 gctattgagg acactatcaa agacaaaata agctccgcca aatttcaaga cagagccccg
    46921 caaaaagtca gattatatcg ccattctgat tttaaaaaaa cgaggattga agagattcaa
    46981 cccaacgaca ttatacaagt gaaaccaggt gagcttatac cattggatag cgtttgtgag
    47041 caagacaccc tcatttatcc caccatcatt accggtgctg tcatacctaa acaatttcgt
    47101 agtggtgaaa aaatacttgc cggaatgcgt ctggcaaatg atgcaaaacc actcaccctc
    47161 cgcgttctaa aaactgcaaa agagtcatac cttgcccgtc ttgatgaaag catagctcaa
    47221 tctgtacttg aaaaggcacc gatagaggta aaaacagggc agcttcttag ctattttatc
    47281 ccatccatta ttgctctctc catcctttca gggatcattg tcggcttgat tgctacacca
    47341 gcacttgcga tacaatgcgc tatttcagta ttggtttctg cttgcccttg tactcttggt
    47401 ttaattattc ctctagcagt aaaaactgga attcataaag gagccgaaca cggtgtgcag
    47461 tttaaaaatt ccagggtatt acaattagca gaacaaattg atgtagtcat ttttgacttg
    47521 aatggcactt taacaaccgg cgttcctgtg gttaaagaat atgcggtatt aaatgatgcc
    47581 gatctttctt ctgagcattt tctgagtatt tgcaatgtat tggaggagca atcaaaacat
    47641 cctttcggaa aagcaataca atcgtttact caaggaatta ataaacaaaa attcaatgtt
    47701 gctaatttgg atcattcaaa ccattcaggc atttcagcag agattaatgg tattcaatat
    47761 actattggca gtaaaacctt aatgcaggaa atagggattt ctacatcttc gcttgaaaac
    47821 caaatgaatc tggttgcggg cgatagcctg gtgtttctag ccagaaacaa acaactatta
    47881 ggttatctgg tcatcaccga cccattacga aaagatgcaa agcaaacagt tgatgcatta
    47941 attaaaatgg gaaaagaagt gcatctatgc actggtgcag atgaaaaaac cgcctatcgc
    48001 tatgcgcaag ctcttggcat taagaaggta catgctaatt gtgtcgctac aactatcaat
    48061 acagaagata gatccaaacc agtttatatt aaatcattac aaaatcaggg actaaaagta
    48121 gctatgattg gagatgcagc taatgatgca aacgctcttg ctgcaagcga cttcggtata
    48181 gcggtactgt ctcaagacag cgatgaaatc acccagcaac acgcctcagc cattatacaa
    48241 agtggaacta tattgcctat agccaatgcc tttgccatat caaaacaaac agtaaacaat
    48301 atcaaccaga atttgttaat tagcttaggg tacaatttga caacagtatt aatagccggc
    48361 ggtctgctag tcgcccttgg ttttgtgcta aaccctgctg ttggcgtagc attaatggta
    48421 acccaggctt gttttgtctt gctcaatgta taccgcttta aagagcaacc cttgcaacat
    48481 ttgcaagaca aagttcagga aaaaccagaa ccggctcctt cttcttatga ggccatgaaa
    48541 aagcattcac ccaatttcca tacagctccc gagattaatc attcgcaagc cattcaatct
    48601 ggaaagcaac cgaccaataa aaataaatca ccttcctttt ggagtgatta ttttcctgaa
    48661 ttatttgagg aagaaaagaa cacaagtacc gaacacctaa tgaataacaa aataggataa
    48721 gccctaaata aagcttggct gaatttgaaa tgaggaaggc caatcacctt cctcaatcat
    48781 aacaatctat tttgatttat tatgctctaa agggtcaaga atgtgatgtc tcttaccaat
    48841 atatgatgac tcataaaaat cgcatgtgaa atgaggtaat cagcaatgta tactgcatca
    48901 ctatatggac aataaataaa ttatatggtc aaattggcaa aattaattaa cttacatttt
    48961 aagaaaactc atgccaacaa cctttgaaga taaatcatcc aaagtcccca gtttaaaatc
    49021 cctggccgct aatgccgttc aaaaaaccaa tgcaaatctc tttttcaggt tgcactcgct
    49081 tgaaactccc cctgagataa aaaaagggtt tattgacaag aaactagagg cattaactca
    49141 cgaattaact gaagactatc aaaccaaggt ggaagccagg aacgaaaaaa ttgaggaatg
    49201 cagttccaat cttagttcta atgagtgttt tgtaaaatgc tctgctttta ccattactac
    49261 attgatggct ggagtgcacg ttggaattta ttacatcctg aaagcagcag ctgtcgaccc
    49321 atcaacgcaa atagcatata tttcgagtat tcccgctact atttgtttct ctatgtgcgt
    49381 tggagtctgc ttgaatcgac aaattaccaa atgcttgagc tcttgtttta cgcccagtgt
    49441 ccccgataaa attactgtcg atttggatga gttgggtagg caatcccatg ttagtccata
    49501 agcggttttc tggattcctt gaaagaaaaa gccgacttat tcgctaaaat ataatttaat
    49561 tacaatgatt cttcactccc tggtaattgt taaaaaatag ggagtggttt catacaccca
    49621 ttttccaccg tatttttttt tgcagtagtc atcaaaaaac tgcaccatgc tgtttacaat
    49681 atcttgatac attttaccgg ggagatcctt gaataatggg gcgccgtgta taaagttttc
    49741 aaataacccg agatcaggta attccacttg gcaatgttct acaacaaaag aaatgctttt
    49801 aaatggcaaa ttatccaatg cccttcggca ttgaacagaa taattctccc gcatcatttt
    49861 agattgcacg acatttttag cgacatcagg gtaaagattt tgaaattgca ggctgtttaa
    49921 cacttcaaac aaagtgcttt gtttgccaga agaaaaaatt gcacaaattt tacctcctgg
    49981 tttcaagaca tgatataaat tggataatga tttttccaaa tcagtccagt gaagacacca
    50041 aaatgataaa atattatcaa atggtccgta aattataggt tcttgaatgt cccctatatc
    50101 aaaacgaacg ttgatactcc tacaatgttt gtttgcataa tcaatcattg tttttgatcg
    50161 atccaagcca agaacatatc catcagggac taaactggct agcatttgag tgaaactgcc
    50221 atcaccacaa ccagcatcga gtaagtaatc attactatgg atagatatct ttgcaagaaa
    50281 cttgctggag atttccctta ggatatagtt accttttgca taattggttg cagaccaatt
    50341 attcaatatg gcaagcaaac tttcattatc cattcgggat ttcttccaaa atgatttacc
    50401 aagagccttt tgtaaattat agagttctcc aacacaattt tctaaaaatt ggaagctttt
    50461 gatgttgtat tctctgagat ctctaaacta gtgatgttat aaacaaggaa taccattagg
    50521 ccattgcaac caatttttct caggataagg taatgaaata tcaaccaaat gcaaagtata
    50581 ggcatgtctg gattgattag aaaaattagc ctggctttta tgtggaactc tgccatgtag
    50641 tataattaaa gcgccttttt taacaggtaa agggacactg ttttcttctg gccaaggact
    50701 tgaatcgaaa ttttcaaaat aaatttcgtt ttctcgacga aacatcctct gctttaaggg
    50761 tgtgatacaa gggcttggaa tcacttccaa acagccattt tccaaagtag catcttctat
    50821 tgcaaaccaa aaccctaaag catcactatc ctcaccaaaa atataggtac tatcctgatg
    50881 gcaaagtact tccgccccta ttcctggctg tttgaatatg tacatcgatt gtaccaatcc
    50941 aagtgttttc aacccaagct gatgtgctat cttcttaatt cgatcatctc gggaatattg
    51001 cctaaaaaca ggatccaatt catgtaaagc atgtccaatt ttattaattg attgattaaa
    51061 aggacgtaga caatcaccgg cttcatcaaa agcacccggc tcaaaaaaat aatgaatttt
    51121 atccccagaa tcaaggaaat attgtttttt tgcatgctcg ttggtttgcg tggaaaaaat
    51181 tgttttaggt atctctgcct gattattttc aagcaatgtt tcaatgcgtt gctttaatag
    51241 atcgcataca gactctgaaa agaaattatt tattaccaaa tatccatttg tgaaaaaaaa
    51301 atcacattgc tcagaagaaa gttgcatcct atgctcctca aaaatcaaaa tcagtacttg
    51361 gggaaatcac cagggtaaag gcaaacagct tttaatgagc ataagcgaaa tgctctcatt
    51421 aaaagccttt cccacacaaa gattaggtat tgttcataag tcctgaaaat atttcagtta
    51481 atttttccat tacatcatct acggtaatta atttcatggc ttcatatcca tggacctgcg
    51541 taccccaaac actcttctct ggactcactc ctaatacagt ttggatagct tgcgaatacc
    51601 tgttaactac caaatgttga aaagtatacg ggccagaaat agagggattg gtaacagcat
    51661 gcaaagccac aacaggcgtg cctaccgctg ccgccatatg agaaggtcct gtatcagggc
    51721 acaagactgc ttttgcttta ctgatcacag ccaacaattg cttaggtttt gtctttccaa
    51781 ctaaattaac cactggaatt tctttcgcta tttcctctgc caatgattta tcatgatcac
    51841 ctggccctcc agttaaaaca actttgacat cccatttaac ttgcgcctgt ttcagaacct
    51901 caatatatcg ctccgccaac caactgcgct ctggtttgct ggctgcggga ttaacaacta
    51961 aaattggcct ttcttgttcc ggcaaatgat tttgagccca ttcataatct gccttggtaa
    52021 tcggtaaatc ccagtggatt attttttcag tgacacctaa aggctcagca aattttaaaa
    52081 agccctcaag tgtatgctcc aatccaggag taatgctttc ctttataaac agacgatggc
    52141 catcattcgc tctgtgagaa tcatagccaa ctttgcgttt agctttaata aatggaataa
    52201 gccaattagt tctaaaactg gcttgaggcg ctaacaacac atcaaactgg cgaggcaaca
    52261 tttgtttcct aaaccgccaa tagtcagcaa gcgttttagg tttatcaata acaataaact
    52321 ccacgccttc cattccttcc accaaataaa aggcaggttt tgaaataatc caggtcaatt
    52381 gagcattagg caatttattt tgcaaggtcc ttatcagtgg gaccaacatc aaaacatccc
    52441 ctaaagctga aagccttata acacaaaccg atttaatcat tctgcaatac aaaacgagag
    52501 ccacaataag ggcaaacctc caccccggtt ttctctatag gcaaataaac cttgggatga
    52561 gcattccata actccatctc atctgttggg caactcaatg gtaattgatg tctatgtacg
    52621 acataatttt ttttagcact tgcaggttct tttgttgttt tcctcatgtt gttcctttgc
    52681 ggcatgatgt caattagaga cagtattatc taatatttcc cttattttcg ctataatatc
    52741 cgtattaaaa cccacatttt gcgaatcata aaataaagcg ccctcccaat gcctcaacca
    52801 ggttaattgc cgtttggcca gctgccttgt tgctgctata cctttttccc tcatagcccc
    52861 ataatcataa tggcctgcaa gatactcgag tatttgccta tatccaacac atcgcatgga
    52921 gggtaagttg atttgaatag gccattttgc ttgcaattgt tgaacttctt ctatgaatcc
    52981 ctcactaagc atcgcgtcaa accgttgcgc gatacgttca tgcaaccagg atctttgctc
    53041 aggaaaaaga gcaagattaa caaaacaata atcaggctgt tcatggggtc cggtcaaata
    53101 ggttgataac gttgaaccgg ttaagtaata aacttccagc gctctttgaa ttctttgcgt
    53161 atcatgagca tgaatgcgag cggctgttac cggatctaac tgacttagct tttgataaag
    53221 aaaatcccaa ccctgtgata atgcttcctc ctccagtcgt ttacgaacag cttcatcggc
    53281 ttcaggcaaa gtggctaacc ctttttgcaa agcattaaaa tacatcatag ttccacccac
    53341 taacaaagga atattccctc ttttgatgat tgcagcacac aaagacaaag cgtctgtaca
    53401 aaactgagct gccgaatagg attcaacagg atctttaata tcaattaaat aatgaggcgc
    53461 actctgtaat tcgtggattg aaggttttgc tgtcccaata ttcatatctc tataaatcat
    53521 tgcggaatca acactaataa tttcaaaagg gaaatgggtt aataactcac acgctaaccc
    53581 tgtctttcct gaagcagttg gtcccattaa acaaaacact agcttattca tgcaataaca
    53641 tccgacaatc atctatagtt aacgctttac atatattatg tttctcttct ttaagagaga
    53701 gctccagcaa cacttgattc atttcaatac gctcttcaac tgttaattgt tttgggtcaa
    53761 atatttgaga gcggctaatt aacgctatca attgctccgt atcaaaaaaa tccagttcgc
    53821 agagaatatt aaaaaaacga cgaacatcta aatagggcgc attcaaaggg atgctgcgaa
    53881 ttaacaagtc atcagcatta gagtactcaa tttgtatccc aacacgagct aactcacgct
    53941 ttaattgctc cggcttatgt atccattggt ttggtaatgg ataacgaatt gagatcaata
    54001 aaggccggct ctccaagggc aatgcttttt gcattagctg tttttctatc cactcttgaa
    54061 acagtgctac taaatctgca aggtaagggc gttgctttat aaaaaccaaa ataaatcgct
    54121 catttaaaat aacccactgg aactctgttt ctggatgata gacagagctg gtttttttat
    54181 accctttgct ggttacagat gatgataatg aattggcact ccagcttggg gataaagctc
    54241 ttgccgcttc atgggactct ctgtcattgc agatatggga aggttccgaa actgtatttt
    54301 gctgctcatt actagaggaa gcagcatacg cgtattccct tgtatctgaa tggtcaagag
    54361 tcgattgcaa cgctttcgtc aattgggaag taaaaaaatc atgtaccagg cggggctgtt
    54421 ggaatctcac ctcatgtttt gtaggatgta cattcacatc cacttcagaa gcaggtagag
    54481 taaaatacaa caaacaaatt ggaaatctgc ctgcatgcaa taagccatca taagcttgtt
    54541 tcactgcatg ttggagtaat ttatccttta ccatgcgttg attcacataa atccactgcc
    54601 tatcattctg gctgcgttgc aaacgatggt tgcttatcca gccataaaga cgcataccac
    54661 tgtgctccac atcaagaaaa atagcttctc tcatgaaagt attacccaaa attcgaccca
    54721 tacgaatggc tttggcttgc tcacttaacg ctggcggtaa tgaaaaaatc agtttcttat
    54781 tatgcttaag cgttaatgca atccgggggg cacttaatgc aaaccgtttc acaaccattt
    54841 caatagcttg aaattccagt ttttcatttt ttaaaaaccg cttacgaact ggggcattat
    54901 agaacaaatc agaaacatca atggtagtgc caatattgcg tgcacaagga gagagagtcc
    54961 tgttctcccc ctctactcgc aacatcattg cattgtcctg ctcctcaggc tttgaaatga
    55021 tagtcacttt ggcaacagag gcaatacttg ccaatgcctc ccctctaaag cccatactat
    55081 caatggaata taaatcatca agcgttctga ttttactggt cgcatgagct gcaacagcta
    55141 aaggcaagtc gtctccaaag attcctgaac cattatcact aactgtaatc tgcagcaatc
    55201 ccccataatt gacatcgaca ccaatcacac tggcaccagc atccaaagag ttttccagca
    55261 gctctttgac tactgaagca ggcctttcta taacttctcc tgctgcaatc tgattggcta
    55321 ttgctggcga aagttgatgt atccttaaga ccatgtgcca ggtatcacta atttttgccc
    55381 gggcataagc cttgcccttg cctgaaggcg gttaattgat tgtaatgcgt tcacagatac
    55441 cctatatcga gcagcgatag caggaagagt ttcacccttt ttaacgatat gaaaatttgg
    55501 agaaatcata gcctcaattc gagtcccatg aggaggatga tcccaaaaat aattttttag
    55561 tccttgaaat attgcctgac tcaatcgctc ctgataggca gggttggtta aatttctttc
    55621 ctcacgggga tttgagataa acccggtttc aaccaaaata gaaggaatat ctggagattt
    55681 cagaacaaca aaacgagctt gctccacttt gttattatgc aaattggtaa aattatccaa
    55741 ttggcctaat acttttcccc ccatttccaa accagcattt attgtcgcag tttgtgataa
    55801 atctatcaaa acagagcgta ctaccccatt actatcatcc aactcaccta aattcactcc
    55861 acctagttca gagtaattct ccttttctgc taaccagcgt gctgcttcac tggtggcccc
    55921 cctttgtgac aaggcaaata ccgaggctcc attagagtga ggattattga atgcatcggc
    55981 atggatagaa atgaatacat caccattata ttttctggca atatccaggc gttgtctaag
    56041 tccaacgtaa taatctcccg atcgtgtcag gactgcgcgc atacctggtt gcctgtctat
    56101 caatcgttta agttttaaag taatggccag cacaacatcc ttctcgttac tacgacgcgg
    56161 acctcttgcg ccaggatctt tgcccccatg ccctgcatcc aacactacaa caacatcacg
    56221 caaaggtttt tttgatgggt tatgaggaac ctgaacaggt gttttatgca cagttattcg
    56281 ttgttctgat ggctggctgg atatttttga ttgggtttgc tggactgaag ctttaatcgt
    56341 tttaacaggc gaactcaact gcctgcgatt atttatgact aaaggagtat cattaacaag
    56401 atcaattctg atcccattaa attccccttt cggtttccat gaagatgagc gcaccttgac
    56461 tggttgtttt acatccatga caagcctgag cgtgttcttg gagggactac cgcttcttac
    56521 ctgctgaata agcgaattga ttaaacccaa ttgactgata ttgacggcta attgtgtatc
    56581 acttaagtct aaaactacac gattgggctg acttaaggta aatagcttat gggtaaatga
    56641 accttgtata gaaagataaa gagacgtcat ggttggttgc tgagaaactg taatattctt
    56701 taattgtgca gaaaatacag caaaagaact tggtagatat aataacagac ttaagaccca
    56761 cgctcgcatc atcatcattc acccgccagg caagctaaaa ttcttttacc cgcgacactt
    56821 aatgcagcta tttgtattaa gcgcccagtc cctttaatta ttaaattaaa ctgaatatcg
    56881 actggaggca atgtttttcc accatgttca ggccattcaa tacaacaaac gctctgattg
    56941 gaaaaatagt ctctaaaacc taaatactcc agttcttctt cctggtgaat acgatacaaa
    57001 tcaaagtgat gaatatgaaa ttgaccacaa ttataacttt caactaatga aaaagttgga
    57061 ctcttgatgg ctgaaattac cccaagtgat tttagcattg cgcgaataat ggttgtcttt
    57121 ccagccccta tatcaccact taaagtaatc accaaaggcg ctgagataca aaacgccaat
    57181 ttttctgcaa atcttttgct ctcttgttca ctaaccaaat ccaaagttat gaaattatca
    57241 ttatcttcaa tagccatatc gagaacacta cataatgtat ctgtttagaa aaagcctgtc
    57301 agccaaaccg ttatacacaa gtattgaaac agaatttgtt acctcagtaa cactcaggtt
    57361 accttcttat caatcatcgc actagataca attcaaaatg cttggaattt catcaagtaa
    57421 gtcgctcgcc aataccccaa cttctcctga tttttcagca accttgtctc cagcaacagc
    57481 atggacccac acccctaact ttgcagcatc ggataaggta aaaccctggg cacaaaaagc
    57541 ggcgataatc ccactcaaaa tatcccccat gcctgcgcta gccatacctg gattgccttg
    57601 agggcacaca aaaatattct tttccgcggt ctgaataacc gtgcctactc ctttcaatac
    57661 cacaacacca ccatattgtt tttgtatagc agatgccgca tggtatctat ccatttgaac
    57721 atctcttgca gtacatgaaa gcaaactggc agcttcgcca ggatgaggtg ttaaaatcca
    57781 attgtcatcc atttggggat attgagaaag taatctcagt gctgaagcat caatgatcat
    57841 gggtaactgg gttgtaatgg cagttaaaaa caaggttttg gcccaatcac tatcaccaag
    57901 cccaggccca ataacacaaa ccgtagcttt tgccaataaa ggcgttaact cccctgctgt
    57961 tttgacaccc cagatcatcg cttcaggaat taagggtaaa gcgctcttcg cataatccgg
    58021 ccaggttgca acagtaacta ctcctgctcc cgtcttgagt gctgccttgg ctgccaagct
    58081 cactgctccc ggcattccag gacccccgcc tatcactaaa acatgtccat aatcaccttt
    58141 atgggagttc ttttttctag ccgcaagagg caatggcaag gtagaatccc tcagcaggga
    58201 agcaaatcgg gttaatttag ttgtacatgc ctctaggttt aaatgagcac aatgaatcac
    58261 accacaataa tctggtccgt ccaaagtata cattcctgtt ttaagtccga taaaagtaat
    58321 tgtggcagtc gcattgacgc aaatattctc tactaatcct gtatccgcat taagaccaga
    58381 tgggatatca atcgataata ccggtaaccc actggcattg atttgatgaa tcgcgttggc
    58441 tattacccca tgcactgctc cttgtaatcc aatacccaat aatgcatcaa caattaattc
    58501 ggcatcgcca tctaaaggtt catctgcgga ttgacaatgt actccagatg ccaatgccag
    58561 gcgtgcagca ttcctggcag cagaaggtaa atcttctatt gatttacatt gatatacagt
    58621 aactgagaat ccttgttcat gagctaatct ggccaagaca taaccatcac ctgcattatt
    58681 gccagcacca cagaagacgg ctatatgccg tacatgcggg tagagtttct gtaagaaaga
    58741 aaatgcctct gcgccagcca gagccatcag ggcattttcg tctttttgat acaaagttgc
    58801 agcttgctgt tcacaaactt taatttgatc acaacgatac agagcatttt ctagcttgtt
    58861 cacctcttgg ctccaaatta acgtaaattt gatataacga ccacaaaata cttttatatc
    58921 gctatgccaa gatcatactg taaaaatcac tgcttcttgg ctacgattcg cttaaaagaa
    58981 accatgccgt ctactttacc aattttagtg atggcttatt ttttgtagca ctttcagagg
    59041 aagaagaagt tttaggtggt ggctcatcgt cttcgtcaac tgaaaaagca attcctctgc
    59101 cattttcttt tgcataaatc tctagtacag cattgggagc aacaaaaatt tgctctgttt
    59161 gcccagaaaa gcgtgccgta aacacaatgc gatcattttc caataaaagt cctcgggtcg
    59221 ctgaaggaga aatgtttaat acaattctat catgctggac atgttccctt ggaacctgca
    59281 cattgggatt tgatgcatct acgagaatgt agggtgttaa atcattatca acaatccaat
    59341 cgtagaaagc acgaatcaag tagggtttgt ttgatgtcat tgccataatt atgctgctct
    59401 tatttgtctc tcagcgtccg tcaaactcgt ttgaaaagaa tcacgtttaa acacacgctg
    59461 catataggaa attattcctt taaaattagg cgcaatttcc aaacctaatt taggtaaccg
    59521 ccataataaa ggagccaatg cacaatccaa aagcgaaaac tcatcactta aaaaataagg
    59581 tttatctgca aagacaggat ctaaactggt taaactttca agtaaattag ctctggcttg
    59641 ctctacagat tcctcccgca aaatagcgtt cattaaataa taccaatcct gttcaattct
    59701 atgcatcatt ttgcgagctt cagcacgggc tactggataa actggtaata atggaggatg
    59761 agggaaacgt tcatccaaat attccataat gattcttgct tcatataata caagttctct
    59821 atcaattaat gtaggaacgg ttccataagg gttaatagac aataggtcgg ctccaggctc
    59881 atcttgcttt actgcaagaa tttcaacatt aactcctttt tctgccagaa cgatacgaac
    59941 ttggtgacta taaacatcat ctacatcaga aaataaagac attatggtgc gctttgcaac
    60001 gattgccatt accttactcc tatcaattca tttaagcctg ttaaaatggc tttttatatt
    60061 ctagtgcaca aaggttgcta ttctatcaca tttttaatct tctgggttac tttgagcaaa
    60121 tcttcttatt ttagcgtgaa ctcggtacaa gaaaccagat atattttctg attctgaagg
    60181 caaccatttg agattgaact ctgtataaaa ggcaaaaatg cagtactttt acaaaactcg
    60241 ggagtcagcg acctattcct attttaattg tttccaataa gctttcttca gtgaataagc
    60301 tgctataagg aaaatacaaa agaacaaaag aacaaaaact cctaatttat aacgaattag
    60361 cttggcaggc tcacctacat aaaccaaaaa attgactaaa tcctttaatg cgctgtcaaa
    60421 ttggccttca aacatctcac ctcgttttac cactaataaa ctatttgcat gcgaattttt
    60481 atctttgacc aaaatgactt ttccaatcaa gggttccaaa acattaggca tagcaacatc
    60541 aggcattagt aaattattag caccaaaagg tctggaaggg tcgctgtaaa aacttttcaa
    60601 ataattatat atccaaacag gccctctatc ccgcgcactt aaagacaaat cgggaggcac
    60661 cataccaaac cattgcttgg catcttcagg cgacatggct atttgaatgg gatcataaat
    60721 agtggcctga gtaaaaatta agttattttt taataaattt tcagcaatct gaccatcaaa
    60781 agtagttaat ccaagatcct gagccatgcg gttataacgc atgtatttga gagaatggca
    60841 tcccgagcaa taattcataa acatctttgc ccctctttgc aaactctctt tatcgtttat
    60901 atcaaccggt acataatcca ttgttaaatt ttctgtaaaa gcagcgtttg acattaaaaa
    60961 tgccaatgca gcaactaata acacctttat tttcatcatt tttcacctat ccgtgtaggt
    61021 actggccggg ttttctcaaa acgactataa ataggcatca agataaagta agaaaaataa
    61081 ataacagcgg caattcgagc taaaaacaac cttactggtg taaccggagt tgtgcccaaa
    61141 tatcctaaaa gtagaaaact aaacgcaaac aaggtaatgc ttattctgga atacattcct
    61201 ttatatctta cagagcgcac attgctttta tcgagccatg gcaaaaagaa aagcaataat
    61261 atggcagcag ccatgcacac caccccaatt agcttatctg gaatagctct taaaatggca
    61321 taaaaaggcg cgagatacca aacaggagca atatgatctg gagtcaccat tggattagcg
    61381 ggtacaaaat tagcatgctc taaaaaatac ccccccattt caggggcaaa aaagacaacc
    61441 gcaaaaaata aaattgaaaa gactatgata cctacaaaat ctttcacagt gtaataagga
    61501 tggaagggta ttccatccaa aggctttcca tccttatcca gggccttctt gatatcaatc
    61561 ccctccggat tgtttgagcc tactttatgc aatgcaacaa tatgcaaaaa cacgaggata
    61621 atcattaaaa tgggtatgcc tatgacatgc aaggcaaaaa atcgctgcaa cgttggattt
    61681 gcaacagagt aatctcccct caaccataca gcaagacctt tgccaatata aggtatcgct
    61741 ccaaataagg aagtaatcac ttcagcaccc cagtaagaca tttgtcccca gggtaataaa
    61801 tagccaaaaa atgcttctgc cattaacaag aggtataaaa acattcccag taaccaaact
    61861 aactcccgtg gtttctgata agaaccataa agcaagcctc taaacatatg caaatagata
    61921 acaataaaaa aagcagaagc tcctgtggag tgcatataac ggagtagcca gccaaaattg
    61981 atgtcccgca taatgaattc aactgaagaa aatgcctggt ccgcgtttgg cgtataaaac
    62041 atggttagcc ataaaccagt aatgatttga ttgactaaaa ctatcagtgc taaagatcca
    62101 aaaaaataaa gaaaattaaa atttttaggc gcataataat tactgaaatg cgctttccaa
    62161 gtgctaacaa gagggaaacg ttcatctaac caattgacta acccattcat gccttaatcc
    62221 ttattttgca tcctcaccta tcacaatggt gtgttcatct aaaaagtgat aaggtggtac
    62281 ttccaaattg ataggggcag gaacgccctt aaatactctg cctgaaagat caaacattga
    62341 cccatgacat gggcaaaaaa agccacctgg ccaatcgggg cctaactcac caagactagg
    62401 tttgtatttt ggggaacaac ccaaatgagt acaaatcccg attaaaacca aaaactcggg
    62461 attaatggag cgaaacttgt tttgcgcata ctccggctgt tgatccacca aagaatcggg
    62521 atccctcagt tgagactcat cttctttttg taactggtta agcatatcct tgcttcgttt
    62581 aattatccat actggtttgc cgcgccattc aataatagcc tgttcaccag gctccattct
    62641 actgacatca actttgactg gagcgcctgc tgcttgtgct ttagagcttg gtaaccagga
    62701 agtaataaag ggagttaagg cgcatgcagc gcctaccccg cctaagatac aggtagtatg
    62761 caacaaaaaa cgacgccgct cttcatccaa ttgttcgtct tgattatgat tacttacatc
    62821 ctgattgaga tcagtcattt cgctcactgc ttaatcatcc tgattatggt ctatgaactg
    62881 gagccagttc gcctaatata actcccgata gttataacaa agaataacaa tttttcaaac
    62941 ggttggataa cttatttctt agaaaataaa aaagcctcgt ataaaacgag gcttggaaaa
    63001 aagtaataga attagcgctt ggagtactga gtagctcggc gtgctttgtg cagccccact
    63061 ttctttcgtt ccacacgtct tgaatcgcga gttaataacc cacgcgccct tagcttgcga
    63121 cggaacgaat cggtacttgg ctcagcgcct tcggccaaac catcttcatc ataagcaacc
    63181 aatgctctgg ctattcccaa acgaacagct cccgcttggc cagatatgcc accaccagtt
    63241 acagtaatgt agacatcaaa cttattaaga agatctactg tttccaaagg ttgcattaca
    63301 atcatgcatg aagtttctcg acagaaatat tcctgcaaag ttctgtcgtt tactttaata
    63361 tcacctttcc caggacgtaa aaatacacgt gcaattgaac ttttcctacg gccggtgcca
    63421 taatattgct tcatttcagc cattatactt attcctcaat ctcaagttgc ttcggttgct
    63481 gcgcagtatg tggatgttcg ctgccagcat atactttcaa cttgcgatac atctctctac
    63541 ctaacgggtt tttgggcaac atgcctttta cagcaagctc aataattttt gttggatttt
    63601 tatcctgtaa tttttcaaag gatgccgatt tgatccctcc aggaaaccct gtatgatgat
    63661 gatacatttt gtttttaaac ttgcgaccag tcacaataac cttttctgca tttgtgacta
    63721 taatgtaatc gcctgtatca acatggggag tatactcagc tttatgcttg ccacgtaaac
    63781 gacgagcaat ctctgtagct aagcgaccca aaactttttc actggcatcg acaacaaacc
    63841 agtcacgctt gacttcattg ccttttgcac taaatgtctt cattaaataa actccgaacc
    63901 gcctcaattg gtaatctttc tatcatggac gggcgatttt aaccaaaaca gaaacaacct
    63961 acaagtaaaa tggataaaaa aatatataca attaatcaaa tctcaataaa aacatcagat
    64021 taatgactaa atgtgaagta tttttcatct tattgacgta agttcatact gaatcaacag
    64081 aaaaccccga tactaatttt catctctttg tttcattaga agagactggg cctgactact
    64141 cgctcagaat taaccaacgc ttaattttat caaaagcctt ttcgtgccag gaaagcacaa
    64201 aaaagtttat ttccctaaat aattatctgt ctagcagaag tggcgctact atcttaccat
    64261 caattaagtg gtacttgatg atttgttcaa tatcttcaaa ggaagaataa gtataccaaa
    64321 ctccttctgg ataaataacg atgcaaggac cagagctaca tcgtccaagg catcctgatt
    64381 tgctgacccg gatttttcca gggccatgta aatcaaattc taacaatttt gatttcataa
    64441 aatcaaaaaa ttcttctcca ccggaattgg cacaacattg cttacccggt gctttctgat
    64501 ttgtgcatat gaatacatgc ttggtgtaat gagacaacaa actactttcc tattttttca
    64561 attttcgttt ttaattttaa acctggctta aaggtaacaa cccttctggc ttccacagga
    64621 atttcttcac ccgtctttgg attgcggcca ggcctttgtg gtttatctct caaagtaaaa
    64681 ttaccaaaac cggataattt aacatgctga ccattttcaa gagcatgacg taattcttca
    64741 aaaaaatttt caaccatttc tttagcaact ggtttattta attttaattc gtcacacaat
    64801 gtttctgcca ttattgcttt gcttagtgcg ttcacgatca ttccctcaaa aggattgaaa
    64861 attcattttc cagtttcttg attatagcac taattgttaa attgatctcg gcatcaacta
    64921 aagttcgagt atcgtcttgt agtgtcatag caacagcaat acttttttta tcttctggaa
    64981 tacctttacc catatacacg tcaaatacgt caaacgattt taaccaatcc tctttaactg
    65041 tatttctgat tactcgctca atctgcatgg cacttatctg cctgtcaacc aggaaagaca
    65101 aatctcgtct tatttgtgga tatttggaaa taggcttata cagaggaata gtcggattga
    65161 ttaatgattc aagattcaat tcaaacaaaa ctacatcctg atcaagatca aaggcatccg
    65221 acaagcgagg atgcagcaca ccgatccagc ctgaatgctt gccgttaatc acaatctgag
    65281 cggactggcc ggggtgaagc gcatgatgag atgattgaat aaattcaaca tcatccagct
    65341 tcaatgaagc aaacaaggat tgtaaatccc cttttaaatc ataaaaatca aataaacggg
    65401 cagattcact ccaattcaaa ttgccttgct cccccattaa caagccagca atacatgaac
    65461 gctcctttaa ttgccctcca tccagatcaa aaaccacgcc tatttcaaaa aatttcactg
    65521 cggtttgttg cctgtggcta ttataaatca tagatgcaat taagcctggc cacattcccg
    65581 cacgcatttg cgataattca gaggaaattg gattaagcag ttccataaac tctttttgtg
    65641 ggtaaagcgc ttcctgcaac tcaggatcaa caaaactata actgatggtt tcatggtagc
    65701 ctttggcact aaaccacgat gaaacgtgtg tcgcaatttt ttccttggca ctgattaaac
    65761 cagcttgtac tgatgtttgc attggctgag cttgtaactt gtcatagcca tataaacgaa
    65821 taatttcttc aaccaaatca gcatcttgtt gcaaatctac gcgatgagaa ggaatggtaa
    65881 cctcaaaaaa atgattggtt tctttgatga tgacaacacc tagaccttcc agtaaatttt
    65941 tcatttcgtt tagagggata gacagcccgg ttaacttttt cacttttgtt gtatcaaata
    66001 aaaaggaaac cgtacctggc aaaaactttt tgtcaaaaga ctctatcact ggcccaggtt
    66061 cgcctcctga gatagataaa atcaattccg tcgctctttc aagcgctttt gattgtaaac
    66121 aagggtccac tccccgctcg aatcgttgtg acgaatcact gaataagcca tattttctgg
    66181 caactccggc aattgtcacc ggattaaaat aggcgctttc caggaaaaca tgttgtgtct
    66241 gctcttgaac tgcgctatta gctcctccca ttattccggc catagccaaa ggcttttctt
    66301 tatcagcgat taccaacaca ttgtcgttta atacaacttc ttgaccatcc agtaattcta
    66361 attgctcgcc tgaagcacta taacgtacat taatttcacc gttaattttt gataaatcaa
    66421 aagcatgcat gggttgccca agctcgagca tgacataatt catcacatct actacagggt
    66481 gtaacgttct aatgccgcct ctacgtaagc gctcagccat ccataatggc gttttcgctt
    66541 caagatttaa attacggata attcggccgc aatatcgggg acatgcctca gaatgaatca
    66601 aattgactcg aagaccatca tcgatagctg gtggcactgt aactattggt tgctctatta
    66661 agggtaattt attgagaaca gccacttccc gcgcgacccc taaaatacta aagcaatctg
    66721 cgcgattcgg agtcaaatca atatcaaata catggtcatc aagggcaaga tattcgcgta
    66781 aatccattcc aattggtgcc tcatcactca gttccattat cccctcggaa tgttctgcca
    66841 gtcccagttc tgtcgcagaa cacaacatgc cctgagacaa ttctcctcgc aatttggatt
    66901 ccttaatttg caaaccgcca ggcaagtgtg ccccaatcat ggccagggca actctcaaac
    66961 caggcctcac attagccgca ccacaaacaa ttttcaacgg cttgtcttta ttgatattca
    67021 cttcacataa ggttaattta tcagcatctg gatgaggttt ggtatttagg acttcagcaa
    67081 caatgacatg agtgaattgg ccagctacag gattcactgc gtcgacttcc aagccagcca
    67141 tggtcaattg ctcagccagt tcttgttcag ttaatgagaa gtttacccat tctcgtaacc
    67201 ataacttact tactttcatt taaagtcctc atctctgtaa ctgttcattt ggctcaaagt
    67261 ttccatccgt taattttgct ctctttatga taatagaga
//
LOCUS       DBNCWK010000004        65701 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.004, whole genome shotgun sequence.
ACCESSION   DBNCWK010000004 DBNCWK010000000
VERSION     DBNCWK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 65701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65701
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.004"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..263
                     /locus_tag="AC06IO_000203"
     CDS             <1..263
                     /locus_tag="AC06IO_000203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873423.1"
                     /translation="FMAMMLLFLLCEPVRNQCLNQFSFYKACKLLSIHSAGLIASFAS
                     KYRLKQFITFFYGELVLFAKKDIKKKHNVSIKTPLLKSNFNA"
     gene            486..737
                     /locus_tag="AC06IO_000204"
     CDS             486..737
                     /locus_tag="AC06IO_000204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873424.1"
                     /translation="MQLNNNTILVGQDGWADSQLGNYDDSQVVLNDSRMIADLFQEKL
                     LGRSQLLAKMQQLTDPEYALVYIYCICAKIALHFHLGVF"
     gene            737..1204
                     /locus_tag="AC06IO_000205"
     CDS             737..1204
                     /locus_tag="AC06IO_000205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873425.1"
                     /translation="MSKSKFFSKDFKGTAFDRACVKIEIDPNYINGVSNKELFNLVVD
                     EKGNSRYEPRKNALVIEDSEISKVDCKVYNIRIARKCCVGNSDYPVWNIDDSVVILVN
                     KDGVTSIHSTEDALKNEWSKPVSHLSVDKFLNCECLHPQIKNDDIQNSLTGPQ"
     gene            complement(1975..3606)
                     /locus_tag="AC06IO_000206"
     CDS             complement(1975..3606)
                     /locus_tag="AC06IO_000206"
                     /inference="COORDINATES: protein motif:HMM:NF015505.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HIO9873426.1"
                     /translation="MASGKQDTSQVVIESIRQSEVIPLLQKYLLANNHALEVNKEGIC
                     NGLAAVYCKYAAQGQEELFIEMLRKIQVLGQQTENTMPQEKIDESLVCKFIAEVLFTF
                     LPQKFNKTLCQDDAGKLLKVDSKDPKETEAKPLKHEYNIGLVAKPEAWGQIFNRIKAE
                     GSVMTVATPTHAIAISVKNGKFHVYDPNHNKIDVFEDTMEEGADPPKVKVSAGTKLAK
                     ALAREVFPENKDKSADLLALTINVFSHPEQPLNHQFPDLSLILKNISKKDTETDKAID
                     QSVNFNGKPFNSLAMAAMQNDEKALRILIEKGAQHPEIALQIAARDNRLKAIDVLLDP
                     SHRHLYKDPEKVMVEACKLALEVGRFEALDKLLQNKEVKDAFTEFMKNSENQNYCLKK
                     VASSGSPQCIEQFINFYEKLQDANVSQLIISNKAIELAQKSGNKSSVELLERRAGLCV
                     TASKDETLIDFPQSEAETNKFIGALKSFFQFIRDVIVNTFSSSNDSSVEKNIETQRDI
                     KGRLGNLTHQASHQLELEDSSTLDTESTSNLGLNN"
     gene            complement(4077..4520)
                     /locus_tag="AC06IO_000207"
     CDS             complement(4077..4520)
                     /locus_tag="AC06IO_000207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873427.1"
                     /translation="MIINEVRETIYLAYNSGQYPLNNWLGEAMDYLFEFMKNNALWLI
                     GVLLSWLFTHIYYKKSLKQQAKEANKENLQLLAGLKKEDAYDKEVFKQQLIEKALEEF
                     IRKGTPIGYINTLDVSDEEKADIYNKACLRKKGRLPKNNPYSVAE"
     gene            4641..5258
                     /locus_tag="AC06IO_000208"
     CDS             4641..5258
                     /locus_tag="AC06IO_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873428.1"
                     /translation="MLKPQDIVILLKILASKQPELLLQKDLATYLCMSASEVHEGMKR
                     LKLSGLLGPVYRESDTFSHTKMIQLPIKAACEECLISGVKYFFPAELGSYTRGIPTSY
                     AAPLFEKYIVLGNDPIPLWPYAEGEHRGLALEPLYRSVPEAIVKHPDQPFYELLVLID
                     AIRTGRARERNIAIKLLKEKIHHEKQTQKSLFESEDAGNSREKTR"
     gene            5188..5892
                     /locus_tag="AC06IO_000209"
     CDS             5188..5892
                     /locus_tag="AC06IO_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873429.1"
                     /translation="MKNKHKRASSNLRMLEIVARKLGNLNDEIVYLGGCTTALFITDP
                     LSLDVRSTLDVDCIIDVISLVQYQKFEKQLKEKGFYRSMNDDVICRWHFDDIILDVMP
                     TDEKILGWGNYWYKEAMNHAISHQITEDLCIKSVTAPYFLATKIEAFKSRGNNDFLGS
                     HDFEDIITVIAGCVEIGKEVQASNDNLKKHLQSVFCELLENDQFIQSLPGHVSDGPVT
                     MQRVQTVIERIKKITA"
     gene            5908..6714
                     /locus_tag="AC06IO_000210"
     CDS             5908..6714
                     /locus_tag="AC06IO_000210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIO9873430.1"
                     /translation="MKLAWLTDIHLNFLDIESRQKFYEEISATKCDALLISGDITEAP
                     CVVELMNEMAKQIKKPIYFVVGNHDYYKGTICDVRSTLTTLSKENDLLFWLPATGIQV
                     LANNTILLGQDGWADDRLGDYQNSPISLNDSRMITDLFQEKLLGKSQLLQKMQQLADL
                     DANALHNDIMQAIESHPRKIIVLTHVPPFKEACLHNGQLSDENWLPYFSSKAMGDVLM
                     PIAANNPLIDFLVLCGHTHSDASYKPVANLTVKVDQAEYYQPVTQEVIIT"
     gene            complement(6731..7462)
                     /locus_tag="AC06IO_000211"
     CDS             complement(6731..7462)
                     /locus_tag="AC06IO_000211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873431.1"
                     /translation="MDISTVKFQNLSPPPNEHVKVWRYISLAKFISLLITRSLYFSRI
                     DCFSDPFEGASTEANIAMRPQLYDGKIPDEKIKELSKCFQVMPQWVYANCWHMSDIES
                     DAMWKLYGSRDGAIAIQSTYKNLADSIKDDSCSIGTVSYIDDMTFIPENNLLTRFFYK
                     RKSFEHEKEVRLLKFDTEFAGKLNKINPCQGTNIEVDIDTLICNIYVTPLCGPWLHEI
                     VIDILKKYNINKPIIESKLCSPPKY"
     gene            complement(7691..7933)
                     /locus_tag="AC06IO_000212"
     CDS             complement(7691..7933)
                     /locus_tag="AC06IO_000212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873432.1"
                     /translation="MRYPFLLNWTPCRFGGERPWFICPAKGCQRRVAILYSGSIYACR
                     HCHQLAYTSQREADYDRALRQAEKIRGEIRLGYWYF"
     gene            8185..8538
                     /locus_tag="AC06IO_000213"
                     /pseudo
     CDS             8185..8538
                     /locus_tag="AC06IO_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8816..9787)
                     /locus_tag="AC06IO_000214"
     CDS             complement(8816..9787)
                     /locus_tag="AC06IO_000214"
                     /inference="COORDINATES: protein motif:HMM:NF020442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HIO9873433.1"
                     /translation="MTASIRTIISALEQLSLEGYVFRGHEDFRYQLTPSAFREHSIAQ
                     MKRDFNISRTLLGEWFNSKEIKKTIASWYSSNPIHSHHPAIYLLKEWCLYIMRYNYSL
                     HLFVKNNPNNVSEQDAASFLLRDAEYWREETTFQQMFERYLPTIINRHSLKDGALIQR
                     ANPYEDLAAVDETLPQHYGIPTAALDWSYNFHVAIHFALGNESEQNSKFLALYALKIL
                     DPQSPIKMLDRNINIENIRAERQEGTFSYFRNPCSFFLQSGTFPSVNYFNQRYKNQLD
                     TCRFELKEFLIERTESNLCYLNELLRERGINKSYLFPEMIDQAELLS"
     gene            9833..10510
                     /locus_tag="AC06IO_000215"
     CDS             9833..10510
                     /locus_tag="AC06IO_000215"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIO9873434.1"
                     /translation="MMQNKFDKICFVLNNYHIITKDANILDLINISTISAGYSFRGKI
                     PELKNSGVYCVQMKDINETYNVNWSTVIETILPSRQSQVSLQFGDILFAARGQRNYAA
                     LINAELKERLAIAAPQFFVIRLNVPDVLPEYIAWFLNQTIAQRYFLSNAEGSTTPSIR
                     KQVLEATPIILPTLKQQKTIMELATTISKEKQLAHKIIANGELLMQTLLNEISQTQLN
                     QEEVTSC"
     gene            10504..12105
                     /locus_tag="AC06IO_000216"
     CDS             10504..12105
                     /locus_tag="AC06IO_000216"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIO9873435.1"
                     /translation="MLNSNALNTTAINADPVNQDAINKALMAACDTFRGTISADTYKD
                     FILTMLFLKYISDVWQDHFDEYQKQYGDEPELIHEMMKNERFVIPEIILKNEDGSVRD
                     QFQATFKNLWERRHEPGNGERIDLCLHAIEEANGTKLRDNAKSVFQDISFNTDKLGEE
                     KQKNTILRHLLEDFAKPELNLRPSRVAGLDIIGNAYEYLIKHFAASGGQKAGEFYTPP
                     EVSSLMATLLDPQPGDSICDPACGSGSLLMKCGRLIRENHHQKNYALFGQEAIGSTWS
                     LAKMNMFLHGEDNHKIEWGDTIRNPKLLDSKGHLMLFDIVTANPPFSLDKWGHEEAEN
                     DHFGRFRRGIPPKTKGDYAFILHMIETLKPKTGRMGVVVPHGVLFRGSSEGKIRQKLI
                     EENLLDTVIGLPEKLFYGTGIPAAILIFKKQKSDDKVLFIDAAKEFKSGKNQNQLSQD
                     NIDKIIETYKQRQSVDRYAYLASLDEIKENDYNLNIPRYVDTFEEEEEIDLIAVRAER
                     LLLKKELAELEFQMETYLKELGYGS"
     gene            12095..13327
                     /locus_tag="AC06IO_000217"
     CDS             12095..13327
                     /locus_tag="AC06IO_000217"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIO9873436.1"
                     /translation="MVPKEWINVSLGDVITYKKGFAFDSSNFQSHGVQIIRISDTTSN
                     SISLKNPIYVSKEVAAKLTAFILHEGDIVLSTVGSRPHLVDSMVGKAVRIPKETEGSL
                     LNQNLVKLIPKKSVIINDYLYLMVKKSRFIYFISTLIRGNANQVSITLNELFKYKFPL
                     PPLIEQKKITQILSTWDKAITTTEKLLANSEQQKKALMQQLLTGKKRFLGFSKEWSNG
                     RLGELCDFKGGSAFKEVQQGKNSGDLPFIKVSDMNLKGNEIFIQNANNWITFEAANQM
                     KAKSFPKWAIVFAKVGAALLLNRRRILQQETIIDNNMMAAIPKKSTDPNFLYQILLLI
                     DFAKLVQEGAIPSINQSDLSAFKIHYPDISEQQKIAIVLSTADREIENIKQKLDCLQQ
                     EKKALMQQLLTGKRRVQV"
     gene            13346..13480
                     /locus_tag="AC06IO_000218"
     CDS             13346..13480
                     /locus_tag="AC06IO_000218"
                     /inference="COORDINATES: protein motif:HMM:NF021471.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIO9873437.1"
                     /translation="MNKGKNQHVVKHSNGWAVKGEGNSKATKVTKTQAEAIEIAENIA
                     "
     gene            13634..15541
                     /locus_tag="AC06IO_000219"
     CDS             13634..15541
                     /locus_tag="AC06IO_000219"
                     /inference="COORDINATES: protein motif:HMM:NF016232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HIO9873438.1"
                     /translation="MHWNVREIFEELIQLDEHQRIEAKRASEIGASVMQTICAFANEP
                     GLGGGWLLLGVDEPNDEHNTHWVSGVENIDKLLAELQNNCRTQFEHPIRIECKHGKFD
                     GKSVVGVFVHELEPNAKPCRFIGKPDKHNKRKTGVWRRGANGDYECTEKELEPILMAK
                     AGISYEQIILPDTTLDDLDENAIALYRKLRAKVRPNAEELQADDLGLLRALRVVKKHG
                     GEYAPNIAGLLLFGKTLSLRGLLPAVRVDYVRVAGTEWVEDPEQRFQYSLDLREPLLT
                     LIPKLENVILDDMPRYFRLEEGQLQRSDQPFLPQKVIREAIVNAVMHRDYHIHQPTIV
                     VRYSNRLEIRNAGYSLKSLDEYDDTGSELRNPILSHVLYDLDFAETKGTGFRTMQRLL
                     NDAGLTKPILLSNRQSNYFKATFLLHQLMNEEQLLWLKQFRDLNLSSDEAKALVLIRE
                     VGAIDNGALRAITDLDTLAASKVLGRLWQQYHLIEKGGSGRNTYYKPTHLLMSYHPSE
                     HQNTDEINGNDSNLMANSNDLNRNGSDLDANSSHLPDALREEIERLTPKSRHIKPVIL
                     KLCQHHSYSADQMATILNRNASALKTKQLAPMCEEGLISYTHPEVRNHPNQAYAITEK
                     GRQWLKNKGQL"
     gene            15538..18810
                     /locus_tag="AC06IO_000220"
     CDS             15538..18810
                     /locus_tag="AC06IO_000220"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIO9873439.1"
                     /translation="MTQLPRFQEEYSAKIPALVVLNNLGWTFLSPEQALALRDGKPNE
                     VVLKAALYDVLRKRRFIYAGKEYPLSDKSIDAIVAEISTPALNEGLTKANERIYNHIL
                     YGITVTEFVGGKKINPTITLIDWHDIQNNSFLFTEEYSVLGAGGVQIRCPDIVCFVNG
                     LPLAIIEAKRPDGKKGPTIEEGISQHLRNQRLGEIPQLFAYSQLLLSINGVDGLYGTQ
                     GTDAKFWATWRDEDIDENQFNQIKNLPLTTQQVDGFFNHRRATDKVWFEKLISQGQLA
                     VTGQDKLLISLLSPQRLLDMTRYFVLFDKKAGKIVARYQQFFGIQRLIERINTPNPDG
                     GREGGVIWHTTGSGKSLTMVFLSRALILHDSLKQCRIVVVTDRVDLERQLSNTFHSGG
                     EFSGKTEFSNAKATSGRRLAEQISKGTERVIFSLIQKFNTAAKLPQCKNLSRDIVVLV
                     DEGHRSQGGENYIRMKQALPNAAFIAFTGTPLLKEDKTTNKFGSIIHAYTIQRAVEDK
                     MVTPLLYEERRPELMVNDRAIDSWFERITEELSDEQRADLKKKYAKKGEIYRSENRIE
                     LVALDIAHHFVKNIDEGLKGQLACDSKLSAIRYKKYLDDVGLFESAVVMSPPDTREGN
                     TEVDESKIPEVTKWWKDNVGNQEEREYTRQVIERFANDEDLKIIIVVDKLLTGFDEPR
                     NTVLYIDKQLKEHNLIQAIARVNRLHEKKKFGLLIDYRGILKELDTTIAKYQDLASRT
                     QQGYDITDLDGLYHQMSSEYKRLPLLYDALWKIFNEVKNKGDFEQLRQVLIPKIQEVD
                     GDLVDTNLKVREDFYDALSEFANCLKVALQSDSFFDDKSITDAQRTHYKETLKQFTSL
                     RQIAKLDAGEIIIYDKYAELVKKLMDKHVVGVQIKEPAGVYEVGKMGLQERPEEWNEE
                     KTRNETDIIKTRIAKMIEQDLRDDPYAQETFSELLKIAIKEADELFEHPLKQYLLFQE
                     FEHDVLNRKLKEIPANFSDNHHAQAYYGVFKLQLPVSFATLTEEEQKKWIDLAFTVDE
                     LINISIAEHSLNQQNTEADIRKKLLPIIFKACKELGFGMEHAKNIIEQVIQIVRVGLI
                     SI"
     gene            18824..19555
                     /locus_tag="AC06IO_000221"
     CDS             18824..19555
                     /locus_tag="AC06IO_000221"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIO9873440.1"
                     /translation="MSTDATYITFGIDKIPFELRPKRQESHRITIKVKPDCQVIVFAP
                     PSATHDEIIVAVNKRAKWVYNKLHIFRSQQEYIIPRQYISGESHYYLGRKYVLKVIED
                     SKAKPQVKLVRGQLQIIAARNIEHRRQLLSEWYKQKAFEVFSKRLDQLLSTAPWIKER
                     PTIHLRAMRSRWGSCAVNGKITLNTHLIKAPTACIDYVILHELCHHSEHNHSDKFYRL
                     LNQVSPNWKQVKQYLDKAANLYLMT"
     gene            complement(19545..20228)
                     /locus_tag="AC06IO_000222"
     CDS             complement(19545..20228)
                     /locus_tag="AC06IO_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9873441.1"
                     /translation="MTELNIKKEIGKRILEARKAKGLTLKALGELAGGLKQTRLTNWE
                     QGVRTPGPEEIKLLAQALDVSPAYLMCLSDTQLHREAKNPSQLIPLLDYRQACEAKLH
                     TGAEISGDKVFISVSTALQPELSTDAFALKITDDSMMPEIRINDVLVIEPCVLPEPGD
                     FVAVKISGKPEAIICQYKKLSYTSSEFELLTLNDNWPNIKVSDDIDVKIIGVLVQNLR
                     CYKSPKVTS"
     gene            20383..21252
                     /locus_tag="AC06IO_000223"
     CDS             20383..21252
                     /locus_tag="AC06IO_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873442.1"
                     /translation="MMDFVINGDELEAMCGLPHIQQLTYLRGIRPYMDVKTGIVGIKR
                     RISHQSISEQLYIEPHQGIKSQSFSRDQVRRSVSGLVRAGLIEVQSEGMHLILKCLLA
                     SQHYSVQNKAAINPPQKAATKPRGESLDNTGLSEVEALKADTVEPPKAAIPHKDNYYI
                     YLLSQFEKFWSLYPEKKSKNNAQAVFEELKPNAELCHTIINALTAQINHRDTMKLQGT
                     WVPPWKYPANWLAQRCWEDELPTDALQEKHHAEHTKNTRKRHTTEDLFCPPCDVEDRE
                     HNNVIQLQRYLQS"
     gene            21218..21580
                     /locus_tag="AC06IO_000224"
     CDS             21218..21580
                     /locus_tag="AC06IO_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HIO9873443.1"
                     /translation="MSSSSKDTFKAERRIDLLFSKFAAFYGHVWRSQFKDEVFLKFAK
                     KEWQEALSDFTDAVLTKAILNCREFYELPPTLPQLLYCCRQIRKQESFYVVKDVYQPA
                     NKAVVSSCLQKCKELLAK"
     gene            21593..21796
                     /locus_tag="AC06IO_000225"
     CDS             21593..21796
                     /locus_tag="AC06IO_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HIO9873444.1"
                     /translation="MLVLTRKAGQQILIGKGLIQMKVLKVDDDIISIGIKAPQHIDID
                     REEIYLKKLQQGQAESSMQKVAP"
     gene            21793..22083
                     /locus_tag="AC06IO_000226"
     CDS             21793..22083
                     /locus_tag="AC06IO_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HIO9873445.1"
                     /translation="MSKLKRWSQSLGNLYLIAVCTLLPTLSHAQSIESIINRTINYLQ
                     GGLARTVGVFCIIIAGYLCLARQKFPKEYFVMILVGMGIIFGGSSLYSTLIG"
     gene            22093..22374
                     /locus_tag="AC06IO_000227"
     CDS             22093..22374
                     /locus_tag="AC06IO_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB3"
                     /protein_id="HIO9873446.1"
                     /translation="MSDLEISPIFNALTRPAMTAGVTFEYHMLNLIVSMCAFIGFSPL
                     YGLIFIPLHVFGWLVCRYDTHFFTICAKRFLLPEAPNTSLWRVRAYEPF"
     gene            22361..24841
                     /locus_tag="AC06IO_000228"
     CDS             22361..24841
                     /locus_tag="AC06IO_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HIO9873447.1"
                     /translation="MNLSKTLKHVYQEAKVSDLFPVTHLNSPSIFESQSGLVGSVLKV
                     DGAAFEIEEADTLNHQRFLLHQALVSLDSRFIIYVTTHRQKISCPLIGEFKQGFAKDL
                     DERYQQRFKDKNLYKNTLYITVVLKGDDSSKTGSWLQWAKSLSIKGNSELSEHHREQS
                     IQTLNRVVEQLQANLNPFGATILGKQDDALGFSELLQFLGLVVNAGQTTSFKHPAYSP
                     PIANSIPQTFRQEAKYPEGHLGQYLSSCQLLFGECIQFQGNTQRDCVFGAMLSLKKYP
                     TNTASILLDSMLSLDCEFIATHTFAPIGRDSALDTISKKRSKLLSAEDKALSQTTALS
                     DLEDGIASETLLLGAHHHTVMLLAPNKSLLDAAILEATKRYGSAGIVVVKETLGQEPA
                     FWSQLPCNQHFITRASLITSQNFVDFCPLHNTQTGFSNENFLGGAVTLLETPSKTPVY
                     FNYHARGSKTNPSKGHAAIFGGNNAGKTTLVNFLDAQMGRFGGRSFFIDRDESSKIYI
                     LASGNSSYIKIESSNPIAMNPLQLPDTSENRSFLKSWLATLVQEESERVIPSNIAEII
                     NDCIDYSFEQLAPEFRTLSHLSQFLPVDFPRWPHLKRWLKRNDSRIDGEFHWFFDNQH
                     DALNLDFDKVGFDVTYLMDQVHSVIATPVYLYLLHRMRQCLDGRLTSFIIAEAWQLFA
                     SPFWEKALREWLPTIRKKNGHFIFDTQSPKTITDSPIKHIVLDNLATLIAFPNPLADR
                     ETYMEHLKLTEAQFEAIKENTPESRIFLYKQDHEAFLCKLDLSGLSQYIRVLSANTQS
                     VKLLDDIMQEVGHEPELWLPVFYERSKK"
     gene            24838..25548
                     /locus_tag="AC06IO_000229"
     CDS             24838..25548
                     /locus_tag="AC06IO_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HIO9873448.1"
                     /translation="MKRAACFLIAAMGISSASHADILGVEDAQLILLAMKQLGELQEQ
                     YRVLSDTYQTARNQLDNLNQLKSMNSGHYGFGDFNNSLDSLQSWQSPVSTWQDALQNL
                     SGGNQQRYQALMDAYEKNHPALDEASYSRFTTPANVMRFKEDKAVNRAVMVQTTESYN
                     EINKHMEALHKLSQQIEKTPNTKGAIDLNSRLITEIGFIQLMSLRLQALISQQAAQEN
                     LSALQDRAEMAKFNRLPK"
     gene            25563..25709
                     /gene="lvhB7"
                     /locus_tag="AC06IO_000230"
     CDS             25563..25709
                     /gene="lvhB7"
                     /locus_tag="AC06IO_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS-associated protein LvhB7"
                     /protein_id="HIO9873449.1"
                     /translation="MKWLLSVLMIALLSGCSKPPDLDSPCHHFGQYCPQYPINDEPIM
                     ELTK"
     gene            25706..26101
                     /gene="lvrD"
                     /locus_tag="AC06IO_000231"
     CDS             25706..26101
                     /gene="lvrD"
                     /locus_tag="AC06IO_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS-associated protein LvrD"
                     /protein_id="HIO9873450.1"
                     /translation="MNTLIKGLIIGLMAATISACHHENPLKTQPAKASVTFLINASAN
                     AEKRLHFAIHKDAYGFAYLECMEGKQSPEIHCDALYHGMITFAKEGHYAGFQTITQAD
                     LTEQKVFESLADEYAETAATTWPHFVSGS"
     gene            26105..27145
                     /locus_tag="AC06IO_000232"
     CDS             26105..27145
                     /locus_tag="AC06IO_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HIO9873451.1"
                     /translation="MNTLQFDNFIMQLAGEVDKLTNHFVFDGYTALATLLKAPLGAMI
                     VLYIILKGYGIALGVIERPQHELFRFSMRVGLIYMLAMNWDLFSSYMRDLFVSGSESI
                     ATTLMQAVHKNPSSGSINQGLQNVLNEILKLGSDLFDAGSLRKLTPYFAGMMVFLSGS
                     VTIGLAFIEIVIAKLMLAVTLCTAPLFILFTLFDQTKSFFDRWLGVLAGFAFVLIFVS
                     SVVGLCIHLLHWVTYAFLGNTDELTAAIWVPIFIVACLCVMGITQAVAIGKSIGGSVC
                     TSAGSAMVGGFIGGSLGAWGFSKSSVQKPINTLRQGLGNASRLMEKGKQLRSASHNVF
                     KQLHKNLRRGAS"
     gene            27142..27858
                     /locus_tag="AC06IO_000233"
     CDS             27142..27858
                     /locus_tag="AC06IO_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HIO9873452.1"
                     /translation="MSKTNWNDYFKRARSWADDQFGRIEQSRNRYQAAFFSAMGLNVV
                     ALIVIGMLAHYQTIVPMLVHHYDNGVTTVEPIENKETPINRAQIESDIARYIQYRESY
                     DASSYRAQFELVHLLSNSTVAKEYLQEQDAANAASPIHALGNHIKREVRIYSINFLDS
                     VLANEKDLHKDHHALAEVVFSLIDIDKTSGKTTSTHYNAMISWRYTNPPDSPETRWKN
                     WDGFEVTRYTRQTRLMEAQA"
     gene            27855..28607
                     /gene="virB9"
                     /locus_tag="AC06IO_000234"
     CDS             27855..28607
                     /gene="virB9"
                     /locus_tag="AC06IO_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HIO9873453.1"
                     /translation="MKKILLSAAVLLLSQGTYAQNNPTSLSTDTRIKKVVYRENDVIP
                     VHGIPFTTTQIQFAKQERVLDIEGGDTTAWMVTYHPQLENMIFIKPTMFDSKTNMTVI
                     TNQRAYYFSLTSAKKLEQEPGKKTYALKFEYPKPQPVHTKNTTPNKTVHPKVVNTAYR
                     FSGSPQLVPRHVFDDGKFTYFELSSQGSVPAIFAVDDQSGKESTVNTRREGKYIVVQR
                     LAPQFTLRQGGLTASVFNAPEINRIKANRRPK"
     gene            28604..29695
                     /locus_tag="AC06IO_000235"
     CDS             28604..29695
                     /locus_tag="AC06IO_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HIO9873454.1"
                     /translation="MSMDDKKREDSLGNRTQVAKNPKHNAMKDKLLLASVVVICIVIG
                     IGGLFPKQKANTVNEPDEKQSLSMALNQNLELIAALKEKNATAQSGYKGGDPRHPPIL
                     KAMQTQTVSKETLARMNAPSTFFNSGGNEVISQTTGGEVQASKTLTGRDGNSEFLNQQ
                     NDITSVSAKRLPHPAMTVPAGEMIPATLETAINSELAGMVRAITTRDIFALEGSRLLI
                     PKGSTLVGQFNTAITQGQSRIFVVWNRLQMTNGVIVTLNSPGSDPIGRSGQAADYIDR
                     HFFERFGSGALLSVLGAYTATGGVNGQDEYNSRSQYRMNIASSFQQAANQTLQQDMQT
                     RPTLQINQGAAINVFVAHDLDFYRVAGRA"
     gene            29697..30701
                     /gene="virB11"
                     /locus_tag="AC06IO_000236"
     CDS             29697..30701
                     /gene="virB11"
                     /locus_tag="AC06IO_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HIO9873455.1"
                     /translation="MEAIKACFSPGATGLLSPLQDFLEDEHVSEILINKPQEVFIERH
                     GQLIRFDIPVLTTQYLRRLFLLIANENKQTLSEASPVLSGNLADGSRVQLVMPPASLH
                     ETLSIRKFTLKQVSFEDYKAKGFFSSARGVGVKECSECIGETDGLLELYDAKNWQEFI
                     RQAILQKKNIIISGGTSSGKTTFLNSCVGQIPLHERLITLEDTYEMDLPHSNIVRLKA
                     LKQIGDHAQKITMQDLVQATLRLRPDRIIMGEIRGRELFDFVSACSTGHSGALATIHA
                     NNPKVAFMRMAQLYKLNQVAGMDEADIYKVLHEVIDVIVQLQKTTDGRRLVEVYYKQA
                     "
     gene            30754..32595
                     /locus_tag="AC06IO_000237"
     CDS             30754..32595
                     /locus_tag="AC06IO_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HIO9873456.1"
                     /translation="MAVLLTGIIQWASPLVLVDLLTHGFYWETTIAGCITALLMSCVL
                     GFLKQGFYRYLSFLISSILLGGMAIFLLGFELYSWIFLSSVQSPSGLMELASLRYQEP
                     ELWHQFMTVEGVVFLMMLLAIALYLFHWLRPDNKALGNAHFSNGFETKKAGFFERQEQ
                     SIIIGKKYAAPLYSNGFEHVLVFAPTGSGKTRSIGIPNLFHYPYSMVCNDVKLTLFKT
                     TSGYRERVLGHRCFCWAPADENRITHCYNPLSLISEDKIQRLTDIQRIAHILMPDNKK
                     SDPIWQQASRKLFKVAVLYLLDTPDRPTTLGEINRLVKQAGFDDWLASVLEETDHLDP
                     EFYRNGYSYLNNHEKTRSSILETFSGYFELFDDPTIDAATARSDFDLRQLRREKITIY
                     IGFTDDDMERLSPLLTLFWQQLISVMIKNIPDPVDEPYPLLCLIDEFSSLGRIERLRR
                     SLKLLREYRVRCVLIMQYIAQTYEQYTHDEAKAFTNIKTKIAFATEDIHDAEYVSKLL
                     GTRTVKVSAGSTSTQTQGYSESKSYNYQAIPLLRPDEVMRLPEDQTLIMRTGHAPVKA
                     SQMIWYLDPEMKHLACGHTEVPCQIVQHHPFIHRSSDRTALPEALEL"
     gene            32694..33491
                     /locus_tag="AC06IO_000238"
     CDS             32694..33491
                     /locus_tag="AC06IO_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HIO9873457.1"
                     /translation="MNHLKIIGTAGLFMMSCAAQANDKELWAHIEALYSKIGALQTQI
                     NSIPQGAPGPMGPAGPKGEQGPAGTYKAGEGIEISNGEIRTTLSHHIGEEYNGGIVFW
                     VDETGQHGLIASKTDLNNNEGIQWRNGESGNKVTNARADGIYAGESNTKLIIAQQTID
                     NQSGSFAALLAANYRVLGDGITPCPTPTPESATCYGGWYLPSAYELTLLHNNLQRQGI
                     STFAPDYYWSSTESGVGEAWLQNFSTGELTASGKASTLGHVRAVSRF"
     gene            complement(33633..34325)
                     /locus_tag="AC06IO_000239"
     CDS             complement(33633..34325)
                     /locus_tag="AC06IO_000239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873458.1"
                     /translation="MDLENNKTDIVVSAIKGSLSAIPCIGSLVAELFCIHIPNQRMDR
                     VTKFIKILSDKVKCLEQSKIEAIFKDPKFIDLLEDATMQSTKATSDERIEKIAQLLSN
                     GMIGEEIEHEKFKRYLFLLNQLTDPEIVKLGGFYYPNQGNSEYYSIHKDILKNIPPSF
                     ASTPEQIETFAIHESHIQHLNQLGLIEAHYKTDRELGIPEFDSITGQMKPSGFKITAL
                     GKSLLKYIENPK"
     gene            complement(34542..37265)
                     /gene="traA"
                     /locus_tag="AC06IO_000240"
     CDS             complement(34542..37265)
                     /gene="traA"
                     /locus_tag="AC06IO_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ti-type conjugative transfer relaxase TraA"
                     /protein_id="HIO9873459.1"
                     /translation="MPLWIRCFSFIKATDGSVRFEREFMAIAFAQVSIHSRAKGHSAI
                     AASSYRSGTKLYDARTGVTHDYSNRHDVIYSDILLPEGSPEAFSDREFLWNQAELAEK
                     RCDAQVCKDIVLALPKELDLVQQIELARRFAQTHFVDKGVPADVAIHDHHDGNPHAHI
                     LITTRRLEKTGFSKYKARDLNPAFAKGFIVEKDYWGEQWRDMQNEYFLEKNLDLTVDL
                     NHLISERHHGKLKDADNHYLLTEKTILQQARQEVFLNDIDNVINHISAKHSVFTRRDV
                     ERLVFKTFQPSDTPQNYLHWVEQIMGHKDLIELGNNERGHLCYTTRHQYIQEAKLRDD
                     IEAMMKNKDVIGDKGIDNIIKNYTLSDEQLEAVRYITEGSQISVVIGRPGTGKSYLLK
                     PIKEHYEQHNYRVIGAALSGKVAKSLQTDTGIASSTIASLTYKLANQQLKLNSNDVLI
                     IDEAGMVDFASMALLIREAKKAGSKVILVGDPDQLKPIHKGEIFRGIAAITGYIELEH
                     IKRQNDLGDRLASMNMAKGMIAEAVDHYHEKGAIVFSETTETAAQNLIQEWQADITNT
                     NLQDSVVLAFTRASVSYLNEQARLALKQKQILGQEEITFQGFEKPLKIAIGERLLFRQ
                     NDKTLGVRNGDLGTVQSIKSNQLQIKLDSGELLTIPCSYTKMDYGYALTVHKSQGMTV
                     KHSKVLIDSKYWDRHLSFVAMTRHKDSLKIYADSINHPTIKVLKQTLSRSNTRDNVID
                     WPLDFATRAGFNPDQLIGRVLNHIAGVGHKIKNAFNYVVHYEAYLLKAGQQDQLHSIE
                     RARIYARQETFKVDTKVIESKNYKISDQALEGLKKDFPQIAEIETLIEKRQQMTGYFA
                     EKVDRQITTISQQLLNNKTFANQVKNKHPEFYLKIQSIYNKQRENAIFHER"
     gene            37440..37646
                     /locus_tag="AC06IO_000241"
     CDS             37440..37646
                     /locus_tag="AC06IO_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873460.1"
                     /translation="MSTKTVSQMEAVLLDRIEKAKIKLDKLQQKHKLEIGSLAYKHGL
                     HQFDIQRLDAAFLKLAGELRNDNA"
     gene            37633..37908
                     /locus_tag="AC06IO_000242"
     CDS             37633..37908
                     /locus_tag="AC06IO_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HIO9873461.1"
                     /translation="MTMLEEIEKEKQLIARCERSLALEKLKKRRADTRHKIELGGLVI
                     KSGLNIFSKAVILGGLDYIFEMAKNNTSYLHLFESRGKELFNITSDN"
     gene            37942..38667
                     /locus_tag="AC06IO_000243"
     CDS             37942..38667
                     /locus_tag="AC06IO_000243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873462.1"
                     /translation="MIKFTLDQALDEIAYRCFRDMADQDYILARVSYRLNLPTNFNWC
                     SLQAIEKYLKCILLLHRIEAKAVNHDLEKALEKIKSVKISDSSSQIEFQDSSIEFIKE
                     INMRGQSRYLGKSIYAIPSDITRLDNCIFDIRRFAIKKIDQIYLEKTRDSKLTHFSDF
                     CLDNGFLEAVVSRNQPAKDDLIFNNSNYGNFGLNMGFSAQNMPASYMGMSNDEYIELY
                     QKLNTLIKVDSFKEIIAQLKNNN"
     gene            complement(38690..39151)
                     /locus_tag="AC06IO_000244"
     CDS             complement(38690..39151)
                     /locus_tag="AC06IO_000244"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIO9873463.1"
                     /translation="MDKYIYDFMAATIEVAVTNPMAPYGAIIVYDDKEILLKSVNSAH
                     NNPLMHGELSVIHRLFENGFDGDVSKLSLYTTAEPCPMCAAAIYWAMIPKVVYGSSIS
                     FLHELFGRQIQVGAEDILSKTPDFYSCHVITGVMVEECNQLFIEAKKLRTT"
     gene            complement(39144..40574)
                     /locus_tag="AC06IO_000245"
     CDS             complement(39144..40574)
                     /locus_tag="AC06IO_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HIO9873464.1"
                     /translation="MNNSIIGKSKTPLQYKVFHTLLGFAVFFSLITVPAEAQLIQIHG
                     ILTSSELLWWPVVFFVLRLIHGVYGFAYLRHAVYAVLLFHAIYVLFLKFAIWLPASSF
                     WKMQEPYTLVLGRDFLYLIKSSLFLWGCALLPIRFASSANHKYYGYIFWVSLVAFCFL
                     DMGWLNMHKNTPDTQIVVPLLIFGLLNIFYNWLSVVIARIEHIESPIQSDRHLFKFQL
                     PQVLKNDGKTFKYHHMLFCSSIVFFIASKTMAAKFISIGFLTINVGGIVFSLAYLAAD
                     MMTDVYGIERTKQMVLFVIFCNLLFVFDVWVTNMLAIGENEPYRAILHNQARMFIASA
                     TAFFLGMTINSTVISLIKSRQRKRGISLKKEFITTVWTRIATSSAFGIIIDVSLFSLV
                     AFYGIVPTEKLASVIVFEDAYKISYEVFLAPVSILMIYFLKVKEKVDIYDELSNLNPF
                     RIDTNYKVSANKFAENYMKPAERNDG"
     gene            complement(40567..40926)
                     /locus_tag="AC06IO_000246"
     CDS             complement(40567..40926)
                     /locus_tag="AC06IO_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873465.1"
                     /translation="MEKWNQGNRFLRFLYQHLYLRFKGQIREPYLIRIDNIYTDPDSN
                     ELTVAFHIANKRVNQDMSVAEFVKTDMIYLVDPRIVFDMGQQFGSHSEKLIVAKKTEP
                     SLKRKCVTTLKRVFIDE"
     gene            complement(40943..41590)
                     /locus_tag="AC06IO_000247"
     CDS             complement(40943..41590)
                     /locus_tag="AC06IO_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9873466.1"
                     /translation="MTTNILSESNNLQLAQTLTDLIERENKLTPAALARKLGIPTNKI
                     TRILNGDVTDPKASTLLQIANYFGITIEQLLGLESIVREGTSNIEPLVQSLPIYEFSH
                     PTQMTNEWYRWSSNEVDGDYFALAIDTDLYEPTFPQNSLLIVNPDMMPDDRSYIVVKR
                     NDSPQHCSIKKYVLEGNQGYLYPINPKLPVELFDRNLYTVAGVILEVHQKLRSKK"
     gene            complement(41687..41893)
                     /locus_tag="AC06IO_000248"
     CDS             complement(41687..41893)
                     /locus_tag="AC06IO_000248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873467.1"
                     /translation="MTINRTNVVDVRIRQIRDVLQGIMCRIDDVLNHSLIDVQFYLLI
                     HMQTLLTARQIYCAADLIKIMTND"
     gene            42029..42238
                     /locus_tag="AC06IO_000249"
     CDS             42029..42238
                     /locus_tag="AC06IO_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HIO9873468.1"
                     /translation="MKWVKLKKYCQDTGDTTNAVHCKRKRGMWLDGLHCKLGPDGNLW
                     INLVEVERWVENGDQATLQKLQAGL"
     gene            42184..43377
                     /locus_tag="AC06IO_000250"
     CDS             42184..43377
                     /locus_tag="AC06IO_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIO9873469.1"
                     /translation="MGGKRRSGDTPKAASGVIVRKWPSGKTTLRISFYYKGVRCFETL
                     KIEATAANIKYAERLRGEILNAIERGNFSYPDYFPNSKRAHIFGHVQSKITIGELLRE
                     FLAEAKSTKEASTYRGYKRVCEGHLLPMFDKVEIQDLQPAMLRKWIRGLQCTTKTVAN
                     ILTPLRAVIEQALVDQYIKENPLNSIIVDKLLNKETKKSDYKPDPFSVDEINAILNES
                     EGQVRLLFQFAFFTGLRVSELIGLRWEDVDWQNQIIHVEETIVYKEAKGPKTEAGVRD
                     VLLLPPALEALEQQKQYTFSQKGRVFHNPQTNKPWETSQQIRRTQWMHILKRAGIRYR
                     NTYQTRHTYASMMLSQGENIMWVSKQLGHVDVEMVIKTYGRWIPDNSTQSGYRPIHDW
                     GSHLG"
     gene            43422..44090
                     /locus_tag="AC06IO_000251"
     CDS             43422..44090
                     /locus_tag="AC06IO_000251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873470.1"
                     /translation="MNNAVECLDLEAIKAKYKENEAHFNLLRSTLISCARKIKIFSII
                     ATQKHTKTELTHEDFWGWLQIETLLPNIILSCYELAKGLKEYNSTFLKPLLPDDSAKS
                     LTEKSKLQYGISALYKQTKKFVKVRNKRIAHLECAVVDSITADLEALYKCLARIDLSL
                     SYIMHYLLNPYLISEQNWSAFDLDKPQDLDSLDAYFEHDPSIRDCKEILMCGADMVPQ
                     NGVG"
     gene            complement(44120..44525)
                     /gene="ssrA"
                     /locus_tag="AC06IO_000252"
     tmRNA           complement(44120..44525)
                     /gene="ssrA"
                     /locus_tag="AC06IO_000252"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(44674..46689)
                     /locus_tag="AC06IO_000253"
     CDS             complement(44674..46689)
                     /locus_tag="AC06IO_000253"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIO9873471.1"
                     /translation="MSTTVGLPQFSHIKIEHFKSHLDALLKNHLEEIDRLLKENHHYT
                     WDNLIYPLDSLADELERFWSPFAHMHAVMDSEAIRESYEACLPLLSAYDAAIGHNQDL
                     YEAIKSIDQHSLNPAQKKIIADSIQDFELSGVALSKANKKRFEAIQSRLAELSSKFEN
                     NVLDATHAYTIHITEAERLAGLPEHALNTAKELAHEKGLDGFVLTLEYPCFQAVITHA
                     EDRALREEMYRAYVTRASDQGPNAGTFDNTPLIDEILSLRHEKAELLGFNNFAELSLA
                     TKMAASTNQVTEFIYDLISRTRDKGKAEFRQLELFAQDKFNLSPVNPWDVAYLSEKRR
                     QDLYSLSQEELRPYFPQPKVMQGLFAIVKKLFGMSIEEIEGVDVWHKDVQCYCIVDES
                     NQTRGYIYTDLFARPHKRNGAWMDSMQSRRKLEDGTVQLPIATLTCNFAKPSANRPAM
                     LSHDEVVTLFHEFGHCLHHVLTQVDYLGGSGINGVEWDAVELPSQFFENWCWDEHALS
                     LLTSHVDTGETLPSALYERLIAAKNFQSAMAMLRQMEFALFDFRIHQEYQTGKASFVP
                     NILADVRSKTSVVPIVPYNRFQHSFSHIFGGGYAAGYYSYMWAEVLSSDAFARFEEEG
                     IFNPKTGHDFLKSILEAGGSRKAADAFVEFRGRPATIDALLRHNGIL"
     gene            complement(46785..48122)
                     /locus_tag="AC06IO_000254"
     CDS             complement(46785..48122)
                     /locus_tag="AC06IO_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873472.1"
                     /translation="MTPIISHLLKIFPELNTQVKTDSLNWTFNTHLKQLGPDFYSKVA
                     RLHPILNMEYSVLCQRLRYNLSSPDYSSPEQIKEQLIDALKLAELLEYTYQHYLVVPR
                     EVVRLRRHKAIYRELLTELSGYSFALDNPEPESLKTSLSLTQAIREKTAQSNWYRIFI
                     SRSKRVINLLDNLDTGSKAFRDFVVLLDKYTNPFLAYLGWCFFAPRLFTNLFLILKHT
                     IPGEWMGEKEKSLDWSDRFYAHLQRRWFELANDSVWFTVGILNCFVLVGALAPLSVYL
                     SLFAFVFDVANTSLRAYIEIGRLQQLQKEYGELFDQEENEDQKKIIKEYQDSISYRIK
                     FEILRSFLSVGGAVAVVIAMALSVPALGFINPVIPLIGAVLLLALWGVSLYLNNKLDE
                     YRPVDNIEKPAPSVINKLGFFASKNEKRESLHLSAKTEKDDEVGELIPTSAFN"
     gene            48308..50386
                     /gene="tkt"
                     /locus_tag="AC06IO_000255"
     CDS             48308..50386
                     /gene="tkt"
                     /locus_tag="AC06IO_000255"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIO9873473.1"
                     /translation="MNGLLADLCLLSDNSASVKCKRVGMNSFTELANAVRMLSIDAVN
                     QAQSGHPGMPLGMADIATVLWKKFLKFNPKNPHWFNRDRFVLSNGHGSMLLYSLLHLT
                     GYNLDINELKNFRQLHSKTPGHPEHGHTPGVETTTGPLGQGLANSVGMALAERVLAST
                     FNHDHYNLVDHYTYTFAGDGCLMEGISHEACSLAGTLGLGKLIVFYDDNGISIDGKVE
                     SWFTDDTTSRFKAYNWQVIGPIDGHDANQIEHAILEASKNNTQPSLIICKTVIGLGSS
                     VAGSEKAHGSPLSAQDINNVREFFNWKHAPFEIPDSIYKQWDHREQGEKEEQQWLQLL
                     HEYRQQFPSEHDEFLRRANGDLPDDWQEISAGFLKQCLNNEKAIATRKASQQCIEFFA
                     PILPEMFGGSADLTGSNNTDWSGSKAITSHNFSGNYLYYGVREFAMAAIMNGLAVHGG
                     FIPYGGTFLVFADYARNAIRLSALMKQRVIYVLTHDSIGLGEDGPTHQPIEHIAMLRM
                     TPGMTVWRPADLMETAVAWQQSLEHHNGPSALLLSRQNLPALAHGTDAADLIKKGGYI
                     IADCEGKPDAILIATGSEVQLAITAAEKAKARGLQVRVVSMPCAERFLAQSDDYKNQI
                     LPGQVRTRIAIEAASSAYWHQFVGFDGAVIGLDCFGVSAPATDAFNYLGITVEKIINT
                     LDTLTKQTMV"
     gene            50468..51460
                     /gene="gap"
                     /locus_tag="AC06IO_000256"
     CDS             50468..51460
                     /gene="gap"
                     /locus_tag="AC06IO_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIO9873474.1"
                     /translation="MTIRVAINGYGRIGRCILRSIFEYNRQNEFQVVAINDLSGIDVT
                     AHLTRYDSTHGPFRSDVSIEGNMLIVDGHGIQVIAERDPAKLPWKQLDIDIVFECTGF
                     FTSRDAAMGHINAGAKKVLISAPAKGVDTTVVYGVNHSVIKASDVIVSNASCTTNCLA
                     PVVKVLHDAIGIESGLVNTVHAYTKDQMLLDGSHKDLRRARSATQSIIPTKTGAAAAV
                     GLVLPELAGKLDGFAMRVPVLNVSVVDLTFQAKRATSVEEVNDIMRKAKSRILQINED
                     PLVSCDFNHNPASAIFDTTETKVIGNLVKVVAWYDNEWGFSNRMIDTAHQMMNC"
     gene            51471..52661
                     /locus_tag="AC06IO_000257"
     CDS             51471..52661
                     /locus_tag="AC06IO_000257"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIO9873475.1"
                     /translation="MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTI
                     KSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADYLRENLEYPVRFVKDYLSGVDVN
                     PGELVVCENVRFNPGEKGNDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
                     AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIA
                     NTFLKAQGFEIGISLYEPDLLDEARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFN
                     KSLSNVAADDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEFPQFAYGTRAIAIAI
                     AESNAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKH
                     VKTN"
     gene            52645..54069
                     /gene="pyk"
                     /locus_tag="AC06IO_000258"
     CDS             52645..54069
                     /gene="pyk"
                     /locus_tag="AC06IO_000258"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIO9873476.1"
                     /translation="MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSAL
                     QLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGD
                     INGVSVAYPNLANELSIGDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
                     KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPII
                     AKIERMEALDHLTDIIREADAIMVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVIT
                     ATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLFPVKVITMVNKICLS
                     AEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWISRQH
                     STVPIFAISANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELVKSGHLEKKGY
                     VLLTRGTQIGTPGGTNCMEIIPVV"
     gene            54160..59787
                     /gene="sdhB"
                     /locus_tag="AC06IO_000259"
     CDS             54160..59787
                     /gene="sdhB"
                     /locus_tag="AC06IO_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdhB"
                     /protein_id="HIO9873477.1"
                     /translation="MLLLSIEVINMPDHNSSDIPLSKQINEVRGLLLKALKAQLDDLI
                     AEKLTPQPSGVPFVHFEKDPPQIATLKKVINCLYHAEEAFKSWESIDTSTLLGKAKAA
                     PKLIRALTQIYKSLGLLDEATPEIRSVIADNYHLLEPLFTQAYTIVQESGWLSEFMEM
                     DVTDKASKVIFQGMDLLGPDIGKSEDTHPLIDAFSKISKLVETVSTLQEKSMTGKEKE
                     RAVELVRSLLDDLDNNPFISKLSISDFEDSKAIKDLLEWFKNIQDDGFDFSKKSIQQY
                     VSWANHYLPTLITFADQLERQNYLKPGSLSTNLCSQIEHLGKQVNGILSEPNFGITER
                     VVSIESLKGKREQQIHNAQANSVRAIMATEKQQAATADFYRILEVYKGMNLSQVTEPD
                     RILLRQLYPQIQTALAHASLDLENLLTSVLNTTGPEKTAPKPKSWWELAKDGLGYVAS
                     YVVNYNVDKILGTRESVDRFLSSQIASEQFKILIAEKAREKLRTGSDKLEETALQYKA
                     EVRVNTIKSTLRKQPSPIESPPSTFVAVKPSKLVNLRGTLTAIQEMQLSTTVNSTRTN
                     VDTLIHRHLPEKYQACFSTLPYQINEKDPDLVKQIKTVENNLFQLEKALKDFEKIDLS
                     YGIMIRLHYFIAIASAASKLKSSVKELSPEGQKALAPLLQQIMTLGSSFSDTNYTAND
                     LSALQQLKTEGQAKPRGPLETSPEKTKTLPEETVKGNEKKPKEKTTELLDYADKISTA
                     RTLLLSKFKSTLSPAIADSLNPQEHGVPFVHLDQDPPQIAAIKKIINSMYHAECALKT
                     WHGIDTSTTLGKIAAAHQGVAALSQVYKSFTLFTEDFSDIHNLIRENHDLIAPVIIEA
                     NKLVKEYGWASQFSELNVAQKLGSILGQGINTVQPKTDEITQTASLVKLFSEIPRLLN
                     NMSSSLDQNAEKSVDELRINSKKIEAIGAVFELFFEENAYLLSIFKGFSAISGLLELN
                     KKIQREGNNLQEVTIRQYQKWLKEGYPNLMMMLDEIETRHYLTPGLLSAPIALELDQI
                     NDKLNEIIETKPDFKLQKIPLSFYMGDKRMERLIAKKTEHCLALIQIEEQKKAVNTFF
                     GILKHYAGQPLSNIKPNELVQLRLAFVKIQSAMANSNLELSNDLVAALNQLETSPPEK
                     TKKIKVTVSQLIKMQPAVENNLNEHKKSCELKIAVIDKALDHVIAKSFELKSDVSKEI
                     NMPQLRRTFLEIEGKKPAIGPGELKPTSVSSLNTVRGNLAYIQELKLSSTVGMIREQF
                     SALTGDNFSTRVQKYLIKPQGKPLHVINDKDPLMVRQIKEVENGLYHLETALKQFEQM
                     KKSDSLVSQTKALVEIANHAHELKMAVERFSPELKQHYGPVVRAALSFSNKVQSINYN
                     KEDWADLQFILNRAHKELLKRKTPRDHRIEKAFFEAGIREVQIDRTESVGKKAAKLGV
                     KYAHLASPQLEKARSYLSSRYKNAFGQQPKLIRSLTREQLANEEFMSAEISRLKEALE
                     EKYGFNLTTIKVILDLLEQIQRAGAQAAEVAGMVNTLVTYDFPKIKENAYRDLLTKLS
                     QEEDYLNLKPGTLINPAMAAVNQLFLSAALELDMPFSKKLSILDDTTYINILVRQTES
                     DLQTLRKKLSEDPSNHEIAFEIEIKNDKLAFLRQQMVLLEDKNPNQTRNVLLDMQFEV
                     SLRNHLVYTTLKAPIAQEYEQIAREYYKKNKNRFLYADDCSKELLQGLKEFEKKHLPD
                     HLIVFEAYDRLRLFSAKLPPKNQDVKEYIENINRELQNRDIPIEQRAIKVKSLPNDSV
                     FIEKLSSADKGTHFLRKFMQFITIITASITEAIKTGGSLIYIYRQKQMEQSIKNIEKS
                     ISLKEELHTIKNTDDASPRKGEGQEGEIEFTKL"
     gene            complement(59886..60956)
                     /locus_tag="AC06IO_000260"
     CDS             complement(59886..60956)
                     /locus_tag="AC06IO_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873478.1"
                     /translation="MRIATLFSAVLFISPLWAAPKFFLSQPALNGVYDIKGTELCSTA
                     KHTLAYLNKGKNYDPQVIHDGKVIKISLDRVKKTLEFICQNQKKLNNPDFVKQHFDFI
                     RWYPDLSQAKQLSANKPLLQNIPKDKILMTKYFVHLAGASTKPSSAKPYPLYALPEDE
                     QNLTLDEANAKPGLIRFRYGKQAILSGALKNQPVSELAYLSRDDLEAALLQGTVVADF
                     GPNAGKKIFNVHRSNNIAYDRTKTPYQQERFWYFKEVDGIKGYGKDAEYKITVNPKVT
                     FAADLAEFGLGKLLMIQYQDKSGRTMTKMGILADTGGAFNNNLYQIDYLAGSFAGKDA
                     LYRATRDLPDYVDAYFMILKQP"
     gene            complement(60957..61649)
                     /locus_tag="AC06IO_000261"
     CDS             complement(60957..61649)
                     /locus_tag="AC06IO_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HIO9873479.1"
                     /translation="MRKQALHPRTAVINKAQKNQSRRARSDALLWLAANFPEAFDNSL
                     RIRPLKIGIMSDILQHAEKAEQVGISKSKLREAVVLFTRRLDYLACLKAREVRIDLLG
                     NPVAEVTEEEAEHASMKIKKRVEKSVKNARKQVNAKAANHSHVNSQPSTVSSVKPMNS
                     FDSHPEPLLPIYPLRSSTYASQNVAMQSAKSPSVVVKHKAPKQYDPDAVARLKEKLGL
                     SRKAEEKKETTE"
     gene            62006..62191
                     /locus_tag="AC06IO_000262"
     CDS             62006..62191
                     /locus_tag="AC06IO_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873480.1"
                     /translation="MRIILSILAIFMPWLVLLIKDNPGGAVVALIMQATLIGWPFASI
                     WAWRAVNPLTPASHSKQ"
     gene            62375..63106
                     /gene="dapB"
                     /locus_tag="AC06IO_000263"
     CDS             62375..63106
                     /gene="dapB"
                     /locus_tag="AC06IO_000263"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIO9873481.1"
                     /translation="MRTRVIVNGANGKMGVLACETLENHEQFEVVAKLSRQDNLGQSI
                     LDTKAQIVVDLTRADCVYENSLTIINHGARPVIGTSGLIEAQIDELTKLCEIKQMGGI
                     IAPNFSLGAILMMMLAAKASEYFSEVEIIEGHHQQKLDAPSGTALKTAEMIAAARKKP
                     KNKLPLKELTPGARGGSHHDINIHSLRLPGLLARQEVLFGNIGETLSITHNSIDRRCF
                     MPGIVLACQKVLNLTNLVYGLEHLL"
     gene            complement(63209..65677)
                     /locus_tag="AC06IO_000264"
     CDS             complement(63209..65677)
                     /locus_tag="AC06IO_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIO9873482.1"
                     /translation="MGKGIILRVLENTILSPQVFDTLERLLPGYKVEYFKEQPDYRKS
                     IARRIDSLHDAFSFILKAYPLDPKHTSLTVATLSTYAAECKASCDLEKLTLEELHLEL
                     ERFTAKLVEAIAIAWKWPKGKAVKEAIASLNEAEQYVLMSRGRSDIATIMPIEMDSET
                     KYVLQYDESLSPVYEQWLTELKQLKEYNFPKTPAWFKNLPPYQQAYYCNLNLSSVDPK
                     KALQHFNTFFGNWGDIAKRSLNLTTELNQIHTNSPPYPSWFNELSPAQQAMIRVLSAT
                     PHEIKSSLKEFKKFMVEQARNDQYASTLSLVPKLPQWYWVLSEKQQYFLEYALKNAEK
                     VEDVVSYLSSRHRTLPAPANYGAHSLYLIDGEGKETLFYDKRYRSSHVASRDSLKLPE
                     DVQQRHVDSNLVKVMEFAKPQQPLLLQTLISPIHAVDYIPTVVTDFLPELPPDLELYK
                     IAREAVTRSKRRHEIFQHNHPFNIAKRYYYTQATDTDSEFLLKTAQKYASSKPGLQAL
                     IDDYKAVLESPLGSATFWDYDGRELFLSSLEELIILNMGGYSYGSCVSGKDRKAVELL
                     HTDAMILYKAKYGNWPKFGIPKEKQERVNFINIVVDLYISRHQHELAGQNAPGSEGIK
                     TPDWYWPNDIAEAINERLGTEKALAYDDRLATDNEVKNISKDLRSFFLPENELHCLLI
                     AKQLGEKMCTMLYDVLSALINEERRFQKSSKDSWKLRWFSDKDVSSTPTGILNIREVM
                     HDENSGNDNVLRIGKIFAAVLNRPESDSSRTTATNSVYDRIRKLLQPLSSEITLQTLA
                     EEAILEWSSLFESSKRENSGLVYV"
BASE COUNT        20370 a        13274 c        13118 g        18939 t
ORIGIN      
        1 aatttatggc tatgatgctt ctgtttttgt tgtgcgagcc tgttaggaat caatgcctca
       61 accaattttc tttttataaa gcctgcaaat tattatcgat tcatagcgcg gggcttatcg
      121 cctcattcgc ttctaaatat agattaaaac aattcattac ttttttttat ggagaactcg
      181 ttctctttgc caaaaaggat attaaaaaga aacataatgt aagcattaaa actcctctac
      241 tgaaaagcaa ttttaatgct tagcttgacg gctatgggcc aaggcccgac aaaatcaatg
      301 ttattctcca aactgattta gtgtgtgtaa tgtatcctaa aaagcccaat cactggttgt
      361 aattcctttt taaatatatc tatcaattaa gtaattgtcg gaccgaggct cgacctacgc
      421 ttgctgaaat gatagaccta actaaatcgg acgtacattt attttagctg cccccattgg
      481 agcccttgca attaaataac aacaccattt tagttggcca agatggctgg gctgatagtc
      541 agctgggaaa ttatgatgat agtcaggttg tgcttaatga cagcagaatg attgctgatt
      601 tgtttcaaga aaagcttctt ggccgttctc aactattagc aaaaatgcaa caacttactg
      661 accctgaata tgcactggta tatatttatt gcatctgtgc taaaatagca ctccactttc
      721 atttaggagt attctgatgt ctaaaagtaa gttcttctct aaagacttca aaggcaccgc
      781 attcgatagg gcttgtgtca aaattgaaat tgatccaaat tatattaatg gtgtctcaaa
      841 taaagaatta tttaatctgg tagttgatga aaaaggtaac tccagatatg aacctcgcaa
      901 aaacgctctt gttatcgaag attcagagat tagtaaagta gattgtaaag tttacaatat
      961 tcgaattgct cgaaaatgct gtgttggtaa ttctgattat ccagtttgga atatcgatga
     1021 ctcagtagta atacttgtaa ataaagatgg cgtaacttcg atacacagca cagaagatgc
     1081 gttaaaaaac gagtggagta aacctgttag tcacttgagt gtcgacaaat ttttaaattg
     1141 tgagtgttta catcctcaaa taaaaaacga tgatatacaa aatagtttaa caggacctca
     1201 ataatattca ctcatcatgg aagacaaatt aaaaaatggt agaaatcaga acagtataaa
     1261 attggaacta atccaaagta agtggaatta gttccaattt agtcaactaa ttcataaatt
     1321 ttgatctttt tataagcctc acttagtttc tttacagttt gtagtaaata aatgtttaac
     1381 tgcatcatca attatttatc ttttaatcat tcacgtgcat aggtggtaat ttatttctcg
     1441 gctttttcag caaaatagcc agtcaaattc tttcgcttat caagtagttt ttggtactca
     1501 gcaagtgcag gatgtttttt tgtaattctt tatagatatc tttttgagaa gtcttcccgc
     1561 ttaattcgtt atttgatgaa tcttgtaagt ctgcagtcaa tttagcacta gatcttaatt
     1621 tggctttact atcttgcaat ccactcaact ggcaatcaac aagataaacc tcgtaattaa
     1681 caacgtaatt atatgcatgc ttgattttat acccactcca gcaatatgat taagcataag
     1741 gtctattagt tgatcaggat taaatcctgc tcaagttgca aaatccaatg gccaatcaat
     1801 aacattatct cttgtgttac tgcgtaataa agtctgtgtc gagtaatgag attcatcagg
     1861 atctgccccc caaggtattc atgatcttca tcgaattttt gtaacctaat tgggacaaac
     1921 attgaatata gatccagtag ttattgaaaa gaactaccga ttaagtaatt cagatcaatt
     1981 atttagtccg agatttgagg tagattctgt gtcaagagta ctactgtctt ctaactctag
     2041 ttgatggctt gcttgatgag ttaaattacc gagtcgtcct ttgatatccc gttgtgtttc
     2101 tatatttttt tccactgagg aatcattaga tgagctgaat gtattgacta ttacatcacg
     2161 aataaattga aaaaaggatt ttaatgctcc aataaattta tttgtttcgg cttccgattg
     2221 agggaaatca atcaaagttt catccttcga agcggtaacg caaagaccgg ctcttctttc
     2281 aagtaattca acagaacttt tatttccaga tttttgagct aattcaatag ccttgttgga
     2341 tattatcaac tgggaaacat tggcatcctg gagtttttca taaaaattaa taaattgctc
     2401 aatacattga ggacttcctg aagaagcaac ttttttcagg caataatttt gattttcgga
     2461 atttttcata aactctgtaa aagcgtcttt aacctcttta ttttgaagta atttatccaa
     2521 agcttcaaaa cgacctactt ccagagcaag tttgcatgct tcaaccatta ctttttctgg
     2581 atctttatat agatgcctgt gactaggatc taacaagaca tcaatagcct ttagccggtt
     2641 atccctggca gcaatctgca atgcaatttc tggatgttgg gccccttttt ctattagtat
     2701 tcgaagagct ttttcgtcat tttgcatcgc agccatagct agggaattaa atggctttcc
     2761 attaaagttg actgattggt ctatcgcttt atctgtttcg gtatctttct tgcttatatt
     2821 tttcaaaata agcgacagat ctgggaattg atgatttagt ggttgttctg gatgactaaa
     2881 tacatttata gtcaaggcta acagatcagc tgatttgtcc ttattttcag gaaaaacctc
     2941 tcgtgccaag gcttttgcaa gcttcgttcc agcactaacc tttactttag gtggatcagc
     3001 cccctcctcc atcgtatctt caaaaacatc tatcttatta tggtttggat cataaacgtg
     3061 aaatttaccg tttttaactg atatggctat agcatgagtt ggtgttgcaa ctgtcattac
     3121 actaccttct gccttgattc tattgaatat ttgtccccat gcttctggtt ttgctaccag
     3181 accaatgtta tattcatgtt ttaaaggttt agcttctgtt tctttaggat cctttgaatc
     3241 tacttttagc aattttcctg catcgtcttg gcataatgtt ttattaaatt tttgagggag
     3301 aaaagtaaat aaaacttctg ctataaattt acaaaccagg ctttcatcta ttttctcttg
     3361 tggcattgtg ttctcggttt gctgaccaag gacttgaatt tttcgtaaca tttcaataaa
     3421 aagctcttct tgtccttgag cggcatattt acagtatact gcagctaacc cattacaaat
     3481 tccctcctta tttacttcaa gagcatgatt attggccaat aaatattttt gaagcaatgg
     3541 tataacctct gattgtcgaa tactctcaat gacaacttgt gaggtatctt gtttccccga
     3601 tgccattatt ctctccaaca actctcatac atatagtata ggcattacaa cgtacaaatg
     3661 ctggattgca acattttaga taagtaaaca gaatttaact ttgtagagaa cgttcagggt
     3721 cagatcttgc cctttgcctt aatccgcaaa agacggcaag cttggaatta aagcttgata
     3781 attatttctc aatgcattaa ttttctttca aaaggaatta agtaagtccg ggattagtga
     3841 tggtaagact tttgcgcatt gagtttgggc tctaccccaa ccaagggggc actttaatca
     3901 ggttagtata aactaacctc ttgactttat tggatgtatc ggatattgaa ttggggcggc
     3961 ggcgacaact taattaatca tttaagttgt tgtttttatt taatattatt gcaggaagat
     4021 tttatagtta tccccagagt tgccctgtat tgtcttctgt aaacaagtta gtcccattat
     4081 tcagcaacac tataagggtt gtttttgggc agccttccct tttttctcaa acatgcttta
     4141 ttgtaaatat cagctttttc ttcatcagaa acatctaagg tgttaatgta gcctataggt
     4201 gttcccttgc ggataaactc ctctaatgct ttttctatga gctgttgctt aaaaacttct
     4261 ttatcataag catcttcttt tttaagtcca gcaagtaatt gcaggttttc tttattagct
     4321 tcctttgctt gttgcttcag acttttttta taatatatat gtgtaaataa ccatgaaagt
     4381 aatactccta ttaaccacaa agcattattt ttcataaatt caaatagata atccattgct
     4441 tctcccaacc aattattcaa tgggtattgg ccactattat aagcaagata aatggtctct
     4501 ctcacctcat taattatcat tgactcatat tctttaagaa aaaagcaagg actgatccta
     4561 tttgatattt gcaattcgcg attcgcaaat tgcaaattta tggtatacaa tataggtaag
     4621 atatgcatag gagactcatg atgctaaagc cacaagacat cgtgatactt ttaaaaatac
     4681 tagctagcaa acagcctgag ctactacttc aaaaagattt agcgacttat ctttgtatga
     4741 gtgcctcgga agttcatgaa gggatgaagc gtttaaaact ttcaggatta cttggacctg
     4801 tttatcgcga atctgatacg tttagtcaca ctaaaatgat acagttacca ataaaagcgg
     4861 cctgcgaaga atgcttaatc tctggtgtga aatatttctt cccagctgag ttaggctcct
     4921 atacacgtgg gattccaaca agctatgcag ccccactctt tgaaaagtat attgttctcg
     4981 gtaatgatcc aatccctctc tggccatatg ctgaaggcga acaccgtggt ctagctttag
     5041 agcctttata tcgttctgtt cctgaagcga tcgtcaaaca tcccgaccaa cctttttatg
     5101 agttattggt gttaattgat gctatccgga ctggacgagc aagagaacga aatattgcta
     5161 ttaagttatt aaaggaaaaa attcaccatg aaaaacaaac acaaaagagc ctcttcgaat
     5221 ctgaggatgc tggaaatagt cgcgagaaaa ctaggtaatc tgaatgacga aatagtctat
     5281 ctaggtggct gtactaccgc attattcata actgatccac tctcgttgga tgtcaggtca
     5341 accttggatg ttgattgcat tattgatgtc atttccttag tccaatatca gaaatttgaa
     5401 aagcaattaa aagaaaaagg cttttaccgc tcaatgaatg atgatgtgat ttgtcgctgg
     5461 cattttgatg acattattct cgatgtaatg cctacggatg aaaaaatttt aggttggggt
     5521 aactactggt ataaagaagc aatgaatcat gctattagcc atcaaatcac agaagattta
     5581 tgcattaagt ctgtcaccgc cccatatttt ttagctacca aaattgaagc attcaaatca
     5641 agaggcaaca atgattttct tggcagccat gattttgaag acattatcac agtgattgct
     5701 ggttgtgttg aaattgggaa agaggtacag gcttcaaatg acaatttaaa gaagcattta
     5761 caatcggtat tctgtgaatt attagaaaat gaccaattta tacagtcatt gcctggtcat
     5821 gtaagtgatg gacctgtcac aatgcagcgc gttcaaacgg ttatagaaag aattaaaaaa
     5881 attactgctt aaaaaacgag tatataaatg aaactagcat ggttaactga tatacatctt
     5941 aattttctcg acattgagtc acgccaaaaa ttttacgagg aaatttccgc gactaaatgc
     6001 gatgcgcttt taattagtgg agatataacc gaagctcctt gtgtggttga actcatgaat
     6061 gaaatggcta agcaaattaa aaagccgatt tattttgtcg ttggtaacca cgattattat
     6121 aaaggaacca tttgtgatgt ccgtagcaca ttaacaacct taagcaaaga aaatgatctt
     6181 ttattctggt tacctgccac tggaatacag gtactagcaa ataatactat tttattagga
     6241 caagacggtt gggctgatga tcggctaggt gactatcaaa atagccctat atcattaaac
     6301 gatagccgaa tgattactga tttatttcaa gaaaagcttc taggtaaatc ccaactgttg
     6361 caaaaaatgc aacaattggc agaccttgat gcaaatgctt tgcacaatga cataatgcaa
     6421 gctattgaaa gccacccccg caagatcatt gtattaactc atgttcctcc attcaaagaa
     6481 gcttgtttgc ataatggaca gcttagtgat gaaaactggc tgccatattt tagttcaaaa
     6541 gcaatgggag atgtattgat gccaattgct gcaaataatc cattgataga ttttttagtg
     6601 ttgtgtggcc acacgcatag tgatgcttct tataagccag tagcgaacct tacagtaaaa
     6661 gtagatcagg cagaatatta tcaaccagta acccaagaag taataatcac ttaagctaat
     6721 tgttttaaac ctaatattta gggggagaac aaagcttaga ttcaataatt ggcttattta
     6781 tattatattt tttaagtata tcgataacaa tttcatgaag ccatggtccg cacagaggag
     6841 taacatatat attgcagatt aaggtatcaa tatccacttc aatattagtt ccttggcagg
     6901 ggtttatttt atttaatttg ccagcaaatt cggtatcaaa tttcaaaagg cgtacttctt
     6961 tttcatgctc gaatgatttt cttttataaa aaaacctagt caataaatta ttctcaggaa
     7021 tgaaagtcat atcatcaata taagagactg ttcctataga gcaactatca tccttgatag
     7081 agtcagccaa atttttatag gtgctttgta ttgcaattgc accatctctt gagccatata
     7141 gcttccacat agcatccgac tctatatcac tcatatgcca acagtttgca taaacccatt
     7201 gaggcattac ttgaaaacat tttgataatt cttttatttt ttcatcagga attttgccat
     7261 cgtacaattg tggacgcatt gcgatatttg cttctgtaga agccccctcg aaaggatctg
     7321 aaaaacaatc aattctggaa aaatatagtg accttgtgat gagtaaagat ataaacttcg
     7381 ccaagctaat atatctccat actttcacat gttcatttgg aggtggagat aaattttgaa
     7441 atttgactgt tgaaatatcc attgttgata gctcattgca tttagaattc aggtgcgcat
     7501 tttacaaaat tgctttctat ggcgatagag ctgcacaaat aaagtggttt atgataaata
     7561 ctcctgaata aagctactcc caaatttcat gtccgctttt gttaatgact taactatttg
     7621 ggcatgttgg cactgggcca aacgttcaaa agtactccaa tgcatccctt taggtttgcc
     7681 cccctccatt tcaaaaatac cagtatccca gcctaatttc ccccctgatt ttttctgctt
     7741 gccttaaagc cctatcataa tcagcctctc gttggctggt atatgctaac tgatgacaat
     7801 gacgacacgc ataaatactg ccggaataaa gaattgcaac tcgcctctga catcccttcg
     7861 ctggacatat aaaccaaggc ctttcaccgc caaatcgaca aggagtccaa tttagtagaa
     7921 atggataacg cataatatct cattccccat aggagttttg gcgacgataa tccaaaacca
     7981 catgatcctt gttcacatga acgttgatag ctgaagtttg ctgaccatca cgagtccact
     8041 gccaagaaaa ggaagtgttc gtcgctaaca agcctctttt atgccaatta cgaacatcaa
     8101 tttttcgcct atcttctgtt atattttttg tgccgcaata taaacggcga ccgcttcaca
     8161 ttccgcccac ttttaatccc atataagcga gactctttcc tcgctgaaaa tccagccatt
     8221 gaagccattt atcacttcca acaacagctt catgagctat taatgaataa aactctaact
     8281 aaagtacggt gccgtaaaat cattcctctc ttcttaaaga tgattcagga cttgaaacaa
     8341 agcccattta aatcattagt tgccctggga aaaaccctcg attcatggaa agaagaaatt
     8401 gcctgtatgt ggcggtttag taaatcaaat ggcatcactg aaggatttca tcggaaaatg
     8461 aaattaatac aacgaagagc ttatgggttt agaaactttg aaaactaccg attaagagtt
     8521 agagtattat gtgcttgata aaatgccccc gtatttggga aagacccgct atttagtgga
     8581 atagtccttt atttacaagg gtttttaaga ctttgttgga tgttgttgaa catttaattg
     8641 gtggaggcgg cgggaatcga acccgctacc actattgttt agattttcat gaaatactat
     8701 caaagaccat gaaaaaccac gtcaattctg agccatcact ctttctccaa aatgcatcaa
     8761 gttgtctttc attgtctgaa attgtcctca gatggtgccg ttttggtgcc gccaattaag
     8821 aaaggagttc cgcttgatca atcatctcag gaaaaagata cgacttattt attcccctct
     8881 ctctcaacaa ctcattaaga tagcataagt tgctttccgt tcgctcaatc aaaaattcct
     8941 tcagttcaaa tctacaggtg tccagctggt ttttatagcg ttgattaaaa taatttactg
     9001 acgggaaggt tccgctttgc aaaaagaaag aacacggatt gcgaaaatag ctgaaagtac
     9061 cttcttgtct ttctgcccta atgttttcta tgtttatatt tctatcaagc attttaattg
     9121 gcgattgtgg atcaaggatt ttaagagcgt ataaagcaag aaatttggag ttttgctcag
     9181 attcatttcc taaagcaaaa tgaatagcca catgaaaatt atatgaccaa tctaatgccg
     9241 cagttgggat cccataatgt tggggcaatg tctcgtctac agcagccaga tcttcatagg
     9301 gattagctcg ttgaatgagc gccccatcct ttaaactgtg gcggttaata atagttggga
     9361 ggtatctttc aaacatctgt tgaaaagttg tttcctccct ccaatactca gcgtcacgta
     9421 ataaaaagct ggcagcatcc tgttcagaga cgttattggg attattttta acaaaaagat
     9481 ggagtgaata gttgtagcgc attatataaa ggcaccattc ttttaataag tatattgctg
     9541 gatgatgcga atgaatagga ttggacgaat accatgatgc tatagttttt ttaatttctt
     9601 tactattgaa ccattcacca aggagcgttc gggaaatatt aaagtctctc ttcatttgag
     9661 caatcgaatg ctctctaaaa gcactgggtg ttaactgata cctaaaatct tcatgtcctc
     9721 tgaaaacata cccttctaag gataattgct ctagtgcaga gattatagtg cgaatgcttg
     9781 ctgtcataaa ataaactcct ttttaaggag aaatactatt acatccttgc caatgatgca
     9841 aaataaattt gacaaaattt gttttgtact caataattat cacatcatta caaaggatgc
     9901 taatattttg gatcttataa acatatcaac aattagcgca ggctattcat ttcgcggaaa
     9961 aattcctgaa cttaaaaact caggcgttta ttgtgttcaa atgaaagaca ttaatgaaac
    10021 atataatgta aactggagca cagtaataga gactatatta cctagccgcc aatctcaggt
    10081 atcacttcag tttggggata ttttattcgc agcacgtggc cagcgtaatt acgccgcatt
    10141 aataaatgct gagctaaaag agcgtcttgc gattgcagca cctcaattct ttgtaattcg
    10201 tttaaatgtt cctgatgttc ttccagaata tatagcttgg tttttaaatc aaactatcgc
    10261 acagcgctat tttctcagta atgctgaggg tagtacaacg ccaagtattc gtaaacaggt
    10321 attagaagcc actccaatta ttttacctac attgaagcaa caaaaaacta ttatggaatt
    10381 agctacaacc attagcaaag aaaaacagtt agcacacaaa ataatcgcta atggtgagtt
    10441 acttatgcaa acactgctta atgaaatcag ccaaacccaa ttgaatcaag aggaagttac
    10501 atcatgctaa atagcaatgc gttaaacact acagccatca atgccgatcc tgttaatcaa
    10561 gacgctatca acaaagcgct gatggcggcg tgtgatactt ttcggggaac gatcagtgct
    10621 gatacctata aagattttat tcttaccatg ctctttctga aatatatatc agatgtctgg
    10681 caagatcatt ttgacgaata tcaaaaacaa tatggtgatg agccggaatt aattcatgaa
    10741 atgatgaaaa atgagcgttt tgtgattcct gaaattattc ttaaaaatga agatggctct
    10801 gttagagatc aatttcaagc aacatttaaa aacctttggg aacgacgtca tgagcccggc
    10861 aatggtgaac gaattgacct gtgcttacat gctatagaag aagcaaatgg taccaaacta
    10921 cgcgataacg ctaaaagtgt tttccaagat atcagtttta ataccgataa actcggtgaa
    10981 gagaagcaga aaaacactat attaagacat ttactagagg attttgctaa acctgaactt
    11041 aacttaagac cttctcgtgt tgcaggacta gatattattg gcaatgctta cgaatatctt
    11101 atcaaacact ttgcagcaag tggtgggcaa aaagccgggg aattctatac tcctcccgaa
    11161 gtatccagct tgatggcaac attacttgat cctcaacccg gtgacagtat atgtgatcct
    11221 gcttgtgggt ctggctcttt attgatgaaa tgtggtcgac tgattagaga aaaccatcat
    11281 caaaaaaatt atgcgctctt tggacaagaa gcgattgggt cgacatggtc gctcgccaag
    11341 atgaatatgt tcttacatgg tgaggataat cataagattg aatggggcga taccattaga
    11401 aatccaaagc tattggatag taaagggcat ttgatgttgt ttgacatcgt cacagcgaat
    11461 ccacccttct cactcgacaa atgggggcat gaagaggctg aaaatgatca ttttggccgc
    11521 tttcgccggg gtataccacc taagaccaaa ggcgattatg cctttatttt acatatgatc
    11581 gaaacactta agcctaaaac tggacgcatg ggtgtagttg tgccccatgg agttctattc
    11641 agaggctcaa gcgaagggaa aatcagacaa aaattaattg aagaaaattt acttgatacg
    11701 gtgattggcc tacctgaaaa actcttttat ggcaccggta ttcctgccgc tatactgatt
    11761 ttcaaaaagc aaaaatcaga tgataaagtg ctttttattg atgccgctaa agaatttaag
    11821 tcgggaaaaa atcaaaacca attgtctcaa gacaatattg ataaaatcat tgaaacttac
    11881 aaacaacgcc aatcggttga tagatatgct tatcttgcaa gcctcgacga aatcaaggag
    11941 aacgattaca acctgaatat tcctcgctat gttgatacct ttgaggagga agaagaaatt
    12001 gacttgatag cggttagagc cgaacgtttg ttgcttaaaa aagaactggc agagcttgag
    12061 tttcagatgg aaacttattt gaaggagcta ggttatggtt cctaaagaat ggataaatgt
    12121 ctccctcggt gacgtcataa catataagaa aggttttgct tttgattcat caaattttca
    12181 gtctcatggt gtacaaataa ttcggatatc agatacaaca agcaactcaa tatctttgaa
    12241 aaaccctatt tatgttagca aagaagttgc tgctaaacta accgctttca ttcttcatga
    12301 gggtgatatt gttttatcta ccgtgggttc tcgcccacat ctcgtagact cgatggttgg
    12361 aaaagctgtt cgaattccta aagaaactga aggaagtcta ctgaatcaga atttagtgaa
    12421 gctaattccc aaaaaatcag taataattaa tgactatcta tatttaatgg ttaaaaaaag
    12481 ccgtttcata tacttcattt ctacactgat tcgtgggaat gcaaaccagg tttctattac
    12541 tttaaacgaa ctttttaagt acaaatttcc acttccaccg cttatcgaac aaaaaaaaat
    12601 cacccaaatt ctatcaacat gggacaaagc catcaccacc accgaaaaat tattagccaa
    12661 cagcgaacaa cagaaaaaag cgttaatgca acagttgctt actgggaaaa agcggttttt
    12721 aggctttagt aaagagtgga gtaatggtcg gcttggagag ctttgcgatt tcaaaggagg
    12781 ttccgctttt aaagaggtac aacaaggaaa aaattctggg gatttaccat ttatcaaagt
    12841 tagcgacatg aatctcaaag gaaatgagat cttcatccaa aatgcaaata attggataac
    12901 tttcgaagcg gcaaatcaaa tgaaggcaaa atcatttcct aaatgggcaa tagtattcgc
    12961 aaaagttggt gcagctttgc tgttaaatcg aaggagaatt ctacaacaag agacaatcat
    13021 tgataataat atgatggccg ccatccctaa gaaatcgaca gatccaaatt ttctatacca
    13081 aattttgcta cttattgact ttgccaaatt ggtccaagaa ggagccatcc catccataaa
    13141 tcaaagtgat ttgagtgcct ttaaaattca ttatccggac attagcgaac aacaaaaaat
    13201 tgccatagta ctttctactg ctgatagaga aatcgaaaat atcaagcaaa aacttgattg
    13261 tttgcaacaa gagaaaaaag cactaatgca acaattatta acaggaaaaa gaagggtaca
    13321 ggtgtaaatt aacaatgagg tttttatgaa caaaggaaaa aatcaacatg ttgtgaagca
    13381 ttcgaatgga tgggctgtaa aaggtgaggg taatagcaaa gccaccaaag ttacaaaaac
    13441 tcaagcagaa gcgattgaga ttgcggaaaa tatagcttga aatcaaaagt cggatacaaa
    13501 gattcatggg gaagatggga aaatacgtgg aggaaatagc tatggtaatg atccatgtcc
    13561 tccaaaagat aaaaaatgaa attatcaata caaattactg gcatacaatt aataaaattg
    13621 aaagagatta cgaatgcatt ggaatgtaag agaaatattt gaagaactca tccaactcga
    13681 tgagcatcaa cgtatcgaag ctaaacgcgc cagtgaaatt ggggcttcgg tgatgcaaac
    13741 tatttgtgct tttgcaaacg aacccggttt aggaggcggc tggctgcttc ttggtgttga
    13801 tgaaccaaac gatgaacata acacacattg ggttagtggc gttgaaaata tcgacaagtt
    13861 attagccgag ctgcaaaaca attgtcgcac acagtttgaa catcccatcc gcattgaatg
    13921 taaacacgga aaatttgatg gcaaatcagt cgttggtgtt tttgtacatg aacttgaacc
    13981 taatgcaaaa ccatgccgtt ttattggaaa acctgataaa cataacaaac gtaaaacggg
    14041 tgtttggcga cgtggggcaa acggtgatta tgaatgcact gaaaaagagc tggagcctat
    14101 tttaatggca aaggctggca tcagctatga acaaattatt ttaccggata ctactctcga
    14161 cgatttggat gaaaatgcta tcgccctgta ccgcaaacta cgtgcaaagg ttcgtcctaa
    14221 tgccgaagag ttacaagctg atgatttagg gttactccgt gcgctgcgtg tagttaaaaa
    14281 acatggtggc gagtatgccc ccaatattgc cgggttactt ttatttggca aaacactttc
    14341 tttgcgcgga ttactacccg ctgtgcgtgt agattatgtt cgcgttgctg gcactgaatg
    14401 ggtagaagat ccagaacaac gttttcagta ctctcttgat ctgcgagaac cattattaac
    14461 tcttatccct aaacttgaaa atgtgatttt agatgacatg ccccgctatt ttcgtttaga
    14521 agaaggacag ctacaacgtt ctgatcagcc gttcttgcct caaaaagtga ttcgcgaggc
    14581 gattgtgaac gcagtgatgc atcgagatta ccatatacac cagccaacaa ttgttgtgcg
    14641 ctatagcaat cgtcttgaga tccgaaatgc aggttattct ttgaagtctt tggacgaata
    14701 tgatgatact ggttctgaat tgagaaaccc gattctttcg catgttttat atgatttaga
    14761 ttttgcagaa acgaaaggaa ccggatttcg cacgatgcag cgtttattaa atgatgcagg
    14821 tttaactaaa cccatattat tatcaaatag acaaagcaat tatttcaaag caactttttt
    14881 actccatcaa ctcatgaacg aagagcaatt gctttggtta aagcagttta gagacttgaa
    14941 tttaagttcc gatgaagcca aagcgttggt cttaattaga gaagtgggag ccatcgataa
    15001 tggtgctctg cgagcaatta cagacttaga tactctggca gcaagtaaag tgcttggtcg
    15061 cctctggcaa caataccact tgattgaaaa gggtggaagt ggacgtaata cctattataa
    15121 accgacccat ttgctgatga gctatcatcc ctctgagcat caaaatacag atgagatcaa
    15181 tggaaatgat agtaacctca tggcaaatag taatgacctc aatcgaaatg gtagtgacct
    15241 cgatgcaaat agtagtcact tacctgatgc tttgcgagag gaaattgaac gattgactcc
    15301 aaagtcacgt catataaaac cagttatttt gaagttatgc caacatcact cgtattctgc
    15361 cgatcaaatg gcgactattc ttaatagaaa cgcgagtgct ttaaaaacga aacaacttgc
    15421 acctatgtgt gaagaaggtt tgataagtta tacccatcca gaggtacgaa atcatccaaa
    15481 tcaagcttac gctatcacag agaaaggaag gcagtggtta aaaaataagg ggcaactatg
    15541 acccaattac ctcgttttca agaagaatac agcgcaaaaa tacctgcgct tgttgttctt
    15601 aacaatttag gttggacttt cctatctcca gaacaagcat tggctttacg tgatggtaaa
    15661 cctaatgagg tagtcttaaa agctgcacta tatgatgtgt taagaaaacg ccgcttcata
    15721 tatgcaggaa aagagtatcc gctttctgat aaatcgattg atgcgattgt tgcagaaatt
    15781 agcacacctg cattaaatga aggattaacc aaagcaaatg aacggatata taaccacatc
    15841 ttatatggaa ttactgtcac tgagtttgtt ggtggtaaaa aaataaatcc aactattacg
    15901 ctgattgatt ggcatgatat tcaaaataat agttttctat ttacagaaga atattctgtt
    15961 ttgggtgctg gtggtgtaca aatccgttgt ccagatattg tttgcttcgt taatggctta
    16021 ccactcgcaa tcatcgaggc gaaacgtcct gatgggaaga aagggccaac gattgaggaa
    16081 gggatatcac aacatctacg caaccagcgt ttaggcgaaa tccctcagtt attcgcctat
    16141 agccaacttt tattatctat taatggcgtt gatggcttat atggaacaca gggcacagat
    16201 gcaaaatttt gggcgacttg gcgagatgaa gatatagacg agaatcaatt taatcaaatt
    16261 aaaaatttac cccttaccac gcaacaagta gacggttttt ttaaccatcg gcgagcaaca
    16321 gataaagttt ggtttgaaaa actcatttcg caaggacaat tagctgtaac aggacaggat
    16381 aaactgctca tcagtttatt atcaccccaa cgattactcg atatgactcg ttatttcgtg
    16441 ttgtttgaca aaaaagcagg gaaaatcgtt gcacgttatc aacagttttt tggtatccag
    16501 cggctgattg aacgaattaa cacaccaaac cctgacggag ggcgtgaagg tggcgttata
    16561 tggcatacga ctggctctgg taaatcgcta acgatggtct ttttatcacg tgcactcatt
    16621 ttgcatgaca gtttgaaaca atgtcgtatc gttgttgtaa ctgatcgcgt cgatctggaa
    16681 agacaactat ctaatacctt tcattctggt ggtgagtttt ctggaaaaac agaattttca
    16741 aatgcaaaag ccacatccgg caggcggctg gcggagcaaa tcagcaaagg cactgaacgt
    16801 gttatttttt ctctcattca aaaatttaat accgcggcca agctgccaca atgcaagaat
    16861 ttaagtcgtg atattgttgt tttagtggac gaaggccacc gctcacaagg cggggaaaat
    16921 tatattcgca tgaaacaggc cttgcctaat gctgccttca ttgcctttac aggtacgccc
    16981 ttgcttaaag aagataaaac aacgaataaa tttgggtcta tcatccacgc ttataccata
    17041 cagcgtgcag tagaagataa aatggtgaca ccgcttttat acgaagaaag acgtcctgaa
    17101 ctcatggtca atgatcgtgc aattgactct tggtttgaac gaataaccga ggagctttct
    17161 gatgaacaac gggctgattt aaagaaaaaa tatgcaaaga aaggcgaaat ttatcgctca
    17221 gaaaaccgaa ttgagcttgt tgctctggat atagcgcatc attttgtaaa aaatattgat
    17281 gagggcttaa aaggtcaact tgcttgcgat agcaagttat ccgctattcg ttacaaaaaa
    17341 tatttagatg atgtaggctt atttgaatcg gctgttgtga tgtccccgcc agatacaagg
    17401 gaaggaaata ctgaagttga tgaatctaaa atcccagaag taacaaaatg gtggaaagac
    17461 aatgttggca atcaagagga acgtgaatac acacgacagg ttatagagcg ttttgccaat
    17521 gatgaagatt taaaaataat tattgtcgtt gataagctgc tcactggttt tgatgaacca
    17581 cgaaatacag tgctctatat tgataaacag ctcaaagagc ataatttaat tcaggctata
    17641 gctcgtgtta accgattaca tgaaaagaaa aaatttggat tattgattga ttatagagga
    17701 atccttaaag agcttgatac caccattgcc aaatatcagg atttagcatc cagaacacag
    17761 caaggttatg acattactga tcttgatggc ctgtatcatc aaatgtctag tgaatataaa
    17821 cgccttcccc tgctctatga cgcattatgg aaaatcttca acgaggttaa aaataaagga
    17881 gattttgagc aactaaggca agtacttatc cccaaaatac aagaagttga tggcgatctc
    17941 gtcgatacaa atctgaaagt tagagaggat ttttacgatg cactgagtga atttgcgaat
    18001 tgcctcaaag ttgccttaca gtctgatagc ttttttgatg ataaaagtat tactgatgcc
    18061 caacgtacac actataaaga gacattaaaa caattcacca gcctgcgcca aattgcaaaa
    18121 ctcgatgcag gtgaaatcat tatatatgat aaatacgccg agcttgtaaa aaagctcatg
    18181 gataaacatg ttgttggtgt gcaaattaaa gagcccgcag gtgtatatga agttggcaaa
    18241 atggggcttc aagagcgacc cgaagaatgg aatgaagaaa aaacaagaaa cgaaacagat
    18301 atcataaaaa cacgcatcgc taaaatgatt gaacaggatt taagagatga tccctatgcg
    18361 caagaaacat tttcagaact tttaaaaata gccataaaag aggcagatga actatttgaa
    18421 catccattaa aacaatattt actatttcaa gaatttgaac atgatgtgct gaatagaaaa
    18481 ctaaaggaaa ttccagcaaa tttttctgat aatcaccatg ctcaagctta ttatggagta
    18541 ttcaagcttc aacttcctgt ttcttttgca actctcacag aagaagaaca aaaaaaatgg
    18601 attgatcttg cctttactgt ggatgaacta attaatattt caattgcgga acattctttg
    18661 aatcaacaaa atacagaagc agacattaga aaaaaattat tacctattat tttcaaagcg
    18721 tgtaaagaat taggttttgg aatggaacat gcgaaaaaca ttattgaaca agttattcaa
    18781 attgttcgtg tgggtttaat ttcaatatga ttaaggataa tcgatgtcta cagatgcaac
    18841 ctatattaca tttgggatag ataaaattcc tttcgagctt cgacccaaac gccaagaaag
    18901 ccatcgcatc acaattaaag tcaaacctga ttgtcaggtt attgttttcg cacctccatc
    18961 cgcaactcat gatgaaatta ttgtggctgt aaataaacgc gcaaaatggg tgtataacaa
    19021 attacatata ttcagatctc agcaagaata tattattcct cgacaatata taagcggcga
    19081 aagtcattac tatctgggaa gaaaatatgt gctcaaagtg attgaagatt caaaagccaa
    19141 accacaagtc aaattagtac gcggtcaact tcaaattatt gcagcaagga atatagaaca
    19201 tagacgccaa ctgttgtcag agtggtataa acagaaagct tttgaggttt ttagcaaaag
    19261 attagatcaa cttctatcaa cagccccttg gataaaagaa cgccccacga tacatttaag
    19321 agctatgcgt tctcgatggg gtagctgcgc agtgaatgga aaaataaccc taaatactca
    19381 tctcattaaa gcaccgacag catgtattga ttacgtaata ctccatgagc tatgtcacca
    19441 ttctgaacat aatcacagcg ataagtttta tcgtctatta aatcaggtta gccccaactg
    19501 gaagcaggta aaacaatatc tcgataaagc ggccaattta tatcttatga cgtaactttg
    19561 ggagacttat aacatctaag attctgaact aaaacaccga taatcttaac gtcaatatca
    19621 tcacttacct taatatttgg ccaattatca tttagtgtta acaattcaaa ttctgaagaa
    19681 gtataagata attttttata ttgacatatg atcgcttctg gcttaccact gattttaact
    19741 gctacaaaat cgcccggttc tggcaaaaca caaggctcaa tcactaagac atcgttgatt
    19801 ctaatttcag gcatcatgct gtcatctgtt attttcaagg caaatgcatc cgtgcttaat
    19861 tctggttgta aagccgtact cacagaaata aaaaccttat caccagatat ctcagcacca
    19921 gtatgcaatt tagcctcgca agcctgtcga taatccaaaa gtggtatcaa ctgactagga
    19981 ttttttgctt ccctgtgcag ttgggtatcc gacaaacaca tcaaataagc aggcgaaaca
    20041 tccaacgcct gcgccagtaa cttaatctct tccggccccg gagtgcgcac accctgctcc
    20101 caattcgtaa ggcgcgtttg ttttaatccc ccagcaagct cgcctagagc ctttaacgtc
    20161 aagcctttag ccttccgtgc ttcaagaata cgtttgccaa tttccttttt gatattcagc
    20221 tcagtcaaat ttacccctaa aaaatgagaa ttaactattg caatatcact tatcgtgata
    20281 taaattagtt tataaattca cgttccgtga ataataatct tttattttta taaagatatc
    20341 acattatgga aataaaaacg aggatgagta gggatattac ccatgatgga ttttgtgatt
    20401 aatggtgatg agttagaggc tatgtgtggg ttgccccata tccagcaact gacttattta
    20461 agagggatac gaccttatat ggacgttaaa accggaatag tcggcatcaa aaggcgcatc
    20521 agtcatcagt ctatttctga gcagctttac attgagccgc atcaaggcat aaaaagccag
    20581 agcttttcgc gcgatcaagt taggcgttcc gtttcaggat tggttcgtgc tggcttaatc
    20641 gaagtgcaat ctgaagggat gcacctgatt ttaaaatgcc ttttggcttc acagcattat
    20701 tctgtgcaaa ataaagccgc cataaacccg ccacagaaag ccgccacaaa gccgcgcggg
    20761 gaatcccttg ataatactgg attatccgag gttgaggcac taaaagccga cacagtggaa
    20821 ccaccaaaag ccgccatacc tcataaagat aattattata tttatttatt atcgcaattc
    20881 gaaaaatttt ggtcgcttta tcccgagaaa aaatcaaaga acaacgcaca agccgtattt
    20941 gaagagctaa agcctaatgc tgaactgtgc cataccatca tcaacgcact gacagcccaa
    21001 attaatcatc gtgacaccat gaaactacaa ggcacttggg tgccaccttg gaaatacccc
    21061 gcaaactggc ttgcccaacg ttgctgggag gatgagttac ctacggatgc attacaggag
    21121 aagcaccatg cagagcatac aaaaaatact cgtaaacgtc acaccacaga agacctgttc
    21181 tgcccaccct gtgatgtcga agaccgagag cacaacaatg tcatccagct ccaaagatac
    21241 cttcaaagct gagagacgta ttgatttgct gttctccaaa tttgcggcct tttatggcca
    21301 tgtttggaga agtcagttta aggatgaagt gtttttgaaa tttgccaaaa aagaatggca
    21361 agaggcgtta agtgatttta cggatgcggt actgaccaag gctattttga attgccgtga
    21421 gttttatgaa ttaccaccta cgctgccgca attgctgtat tgctgcagac aaataaggaa
    21481 acaagagtct ttttatgtgg ttaaggatgt gtatcaacct gcaaacaaag ccgttgtcag
    21541 ttcctgtttg cagaagtgca aggaactttt agcaaaataa ccggagaaca ccatgttggt
    21601 tttaacacga aaagcagggc aacagatttt aattggtaag ggcttaattc aaatgaaagt
    21661 gctcaaggtc gatgatgaca tcatcagcat tggcattaaa gcaccccagc atattgatat
    21721 tgaccgagaa gagatttatc tgaaaaaact ccaacaaggg caggctgaat cttccatgca
    21781 gaaggtggca ccatgagcaa attgaagcga tggagccaat cccttggcaa cctctattta
    21841 atagcagttt gtactttact gccaactttg tcacatgccc aatcgataga aagcatcatc
    21901 aacagaacca tcaattacct tcagggtggg cttgccagaa ccgtgggcgt tttttgcatc
    21961 atcatcgcag ggtacctgtg tttggcgcgt caaaaatttc ctaaagaata ttttgtgatg
    22021 attcttgtgg gcatggggat catttttggc ggatcttccc tgtattcaac cctcattggc
    22081 taggaggttc ttgtgagcga tttggaaata agtcctatat tcaatgcgct gacccgtccg
    22141 gcaatgacgg ctggggtgac ctttgaatac cacatgttaa acctcattgt ctcgatgtgt
    22201 gcatttattg ggttctctcc actgtatggg ctgattttta ttccattgca tgtttttgga
    22261 tggctagtgt gtcgatacga cacccatttt tttaccatct gcgcaaagcg tttcttattg
    22321 ccagaagctc ccaatacatc actatggagg gttcgcgctt atgaaccttt ctaagacatt
    22381 aaagcacgtt tatcaggaag caaaagtgtc agatttgttt cccgtaacgc atcttaatag
    22441 ccccagcatt tttgaatcgc aatcaggctt ggtcggatcg gtgcttaagg tggatggtgc
    22501 ggcttttgaa atcgaagaag cggataccct aaatcaccaa cgctttttgc tccatcaggc
    22561 attagtgagt ttggattcgc gtttcattat ttatgtgacc acacatcgac aaaaaatatc
    22621 atgccctttg attggggagt ttaagcaagg gtttgccaag gatctggatg agcgttatca
    22681 acaacgattt aaagataaaa acctgtataa aaataccctg tacatcaccg ttgtacttaa
    22741 aggagatgac tccagtaaaa cgggatcttg gttgcagtgg gcaaaaagcc tctcgattaa
    22801 ggggaatagt gagctgtccg aacaccacag ggaacaaagc atccaaacct taaatcgtgt
    22861 tgttgagcaa ttgcaagcga acctcaatcc tttcggggca acgatacttg gcaagcagga
    22921 tgacgcatta ggttttagcg agctgcttca gttcctcggg ttggtcgtga atgcaggtca
    22981 aacaacttca ttcaaacacc ctgcgtacag ccctccaata gctaactcga tccctcagac
    23041 attcagacaa gaagccaaat accctgaagg acatctcggc caatatttaa gtagttgtca
    23101 gcttttattt ggagagtgca tccagtttca gggcaatact caaagggatt gtgtgtttgg
    23161 tgcaatgctg tccctcaaaa aataccccac caacaccgcc agcatcctct tggactccat
    23221 gttatctctg gattgtgaat ttattgccac ccacactttt gcccccattg gccgtgacag
    23281 tgccttggat acgatttcaa aaaaacgatc caagctctta agtgccgaag acaaggcatt
    23341 aagccaaaca actgcattaa gtgatttgga agatggcatt gccagtgaaa ccctgttact
    23401 gggtgcacat catcatacgg tgatgttatt ggctccgaat aaatcactat tggatgcagc
    23461 catattggaa gccaccaaac gctatggctc tgctggcatt gttgtggtga aagaaacgct
    23521 tggacaagaa cctgctttct ggagccaatt accttgcaac cagcatttta taacacgagc
    23581 ttccctgatt acctctcaaa atttcgtgga cttttgccca ttacataaca cccaaacggg
    23641 cttttccaat gagaactttt taggtggcgc ggttacgctt ctggaaactc cttcgaaaac
    23701 gccagtttat tttaactacc atgcacgagg atctaaaacc aacccctcca aaggacatgc
    23761 ggccattttc ggaggaaata acgcagggaa aaccacactg gtaaattttc tggatgcgca
    23821 aatgggtcgt tttggtggaa gaagtttttt cattgatagg gatgaatctt ccaaaattta
    23881 tattttagct tctggtaaca gctcctatat caaaatcgag tcatccaacc caatagccat
    23941 gaatcctttg caactcccag acacctcaga aaatcgctcc tttctgaaat catggcttgc
    24001 aaccttggta caagaagaaa gcgaacgtgt tatcccaagc aacattgctg aaatcatcaa
    24061 tgactgtatt gattacagct ttgagcagct tgcaccggag ttcagaacgc tgagtcactt
    24121 gagccaattt ctacctgttg attttccacg ttggccgcat ttaaaaagat ggttaaaacg
    24181 taatgattcc agaattgatg gcgaatttca ttggtttttt gacaaccagc acgatgccct
    24241 aaatcttgat tttgataagg tggggtttga tgtgacctac ctgatggatc aagtacacag
    24301 cgtcatcgca acgcctgtct atttgtattt gctgcatcgt atgcgccaat gcctcgatgg
    24361 acggctaacc tcttttatta tcgccgaagc atggcagctc tttgcatctc ccttttggga
    24421 aaaagcactt cgggaatggc tgcctacgat tcgaaagaaa aacgggcatt ttatttttga
    24481 tacccaatcc ccaaaaacga ttacggattc ccccattaaa cacattgttc tggataatct
    24541 ggctaccttg attgcctttc ctaatccact tgcagacagg gaaacctata tggagcattt
    24601 aaagctgacc gaagctcaat ttgaggcgat taaagagaac acgcctgaat cacgtatttt
    24661 tctttataaa caagatcacg aagccttttt atgcaaactg gatttaagcg gtttaagcca
    24721 atacatcagg gtactttctg ccaacaccca atccgttaag ttgctggatg acatcatgca
    24781 agaggtcggt catgaacctg agctttggct tcctgtcttt tatgaaagga gcaaaaaatg
    24841 aaacgagccg cctgtttctt aattgcagcg atgggcatca gcagtgccag tcatgcagat
    24901 attctgggtg tggaggacgc acagttaatt ttattggcaa tgaaacaact cggtgagcta
    24961 caagagcaat atcgggtgtt atcagatacc taccaaaccg ccagaaatca attggacaac
    25021 cttaaccagc ttaaatccat gaattcagga cattatggat ttggcgattt taataacagc
    25081 ctcgattcat tacaaagctg gcaatcccct gtaagcactt ggcaggatgc gttgcaaaac
    25141 ctctctggtg gcaaccaaca acgatatcag gcactcatgg atgcctatga aaagaaccat
    25201 cccgcactgg atgaggcgag ctactctcga tttacaacgc ccgctaatgt catgcgcttt
    25261 aaagaagata aggcggtcaa tcgtgcagtg atggtgcaaa ccaccgaaag ctacaacgaa
    25321 atcaacaaac atatggaggc acttcataag ctctcgcaac aaattgaaaa aacacccaac
    25381 accaagggag ctattgattt gaattcccgt ctcattaccg aaattgggtt cattcaatta
    25441 atgagcttaa gacttcaggc gttaatcagc cagcaagcag cacaggaaaa tttatctgcg
    25501 ctccaagaca gagctgaaat ggcaaaattt aatcgattac ccaagtgaca aggagtaacc
    25561 acatgaaatg gcttttgagt gtattgatga ttgcattatt aagtggctgt tcaaagcccc
    25621 ctgatctgga ttctccctgc catcactttg ggcagtattg ccctcagtat cccattaacg
    25681 atgaacccat tatggaactc acaaaatgaa cacactcata aaaggtctaa taattggttt
    25741 aatggctgca acaattagcg cgtgtcatca cgaaaacccg ttaaaaacac agcctgccaa
    25801 ggccagtgtt acgtttttga ttaacgcctc ggccaatgct gaaaagcgat tgcattttgc
    25861 cattcacaag gatgcttatg gctttgccta tctggaatgt atggagggca agcaaagtcc
    25921 agaaatccac tgtgatgcgc tgtatcatgg catgattact tttgccaaag agggtcatta
    25981 tgcgggcttc caaacgataa cgcaggctga tttaaccgag caaaaagtgt ttgaatcact
    26041 agctgatgaa tatgccgaaa cggcagccac cacatggcct cattttgttt cgggaagtta
    26101 atccatgaat acactacagt ttgataattt cattatgcag cttgccggtg aagtcgataa
    26161 gctgaccaac cattttgttt ttgatggcta cacggctctt gcaacactgc ttaaagcccc
    26221 tttgggagcg atgattgttc tttacatcat tttgaaaggc tatggaattg ctctgggtgt
    26281 tatcgaacga cctcagcacg aattgtttcg attcagtatg agggttggat tgatttacat
    26341 gttggctatg aactgggatc tgttttcatc ctacatgcgc gatctgtttg tatcgggtag
    26401 tgagtccatt gccactacat taatgcaggc cgttcataaa aatccctcaa gcggctcaat
    26461 caatcagggt ttgcaaaatg tattgaatga aatcctcaaa ctaggttcgg atttatttga
    26521 tgcaggatca ctcagaaaac tcacgcccta ttttgcgggc atgatggtgt ttttatcagg
    26581 gagtgtcacg ataggtctgg cttttattga aatcgtcatc gccaagctga tgttggctgt
    26641 gaccttgtgc acagcaccct tattcatcct gtttacgctt tttgaccaaa ccaaatcctt
    26701 ttttgatcga tggcttggcg tactcgccgg atttgccttt gtgcttattt ttgtgtccag
    26761 cgtggtgggg ctatgcatcc atttattgca ttgggtcact tatgcctttc tgggcaatac
    26821 agatgaactg acagcagcca tttgggtgcc gatttttatc gtggcctgtt tatgtgtcat
    26881 gggcatcact caagccgttg ccattggcaa aagtattggt ggttcggtat gtacttctgc
    26941 tggctctgcc atggtgggag gctttatcgg tggcagttta ggggcatggg gattttcgaa
    27001 gtcttccgtt caaaaaccaa taaatacctt gcgccaagga ttaggcaacg catcacgtct
    27061 catggaaaag ggcaaacaac tgagatcagc cagtcacaat gtattcaagc aactgcataa
    27121 aaacctgcga agaggagcat catgagtaaa accaattgga atgattactt taagcgtgcg
    27181 cgaagttggg cagacgacca gtttggccga atcgaacaat cacgaaaccg ctatcaagct
    27241 gcatttttct cagccatggg cttgaatgtg gtagctctca tcgtgatagg gatgctcgcg
    27301 cactatcaaa ccatagtacc gatgctggtt catcattacg acaatggggt aactacggtt
    27361 gaacccatag agaataagga aacaccgata aatcgcgcac agattgaaag cgacatcgcc
    27421 cgttacattc aataccgtga atcttatgat gcctcaagct atcgcgctca atttgaattg
    27481 gtgcacctgt tatccaacag cacggtggcc aaggaatatt tacaagagca agatgctgca
    27541 aatgctgcct cacccattca tgctttgggg aatcacatca agcgagaggt tcgcatttac
    27601 agcattaatt tccttgattc cgttttggca aatgaaaaag accttcacaa agatcaccac
    27661 gccttggcag aagtggtttt tagtctcata gacatcgaca aaaccagcgg aaaaaccacc
    27721 tctacgcatt acaacgccat gatttcatgg cgatacacca atccccctga ttcccctgaa
    27781 acccgttgga aaaactggga tggatttgaa gtcacacgct atacaagaca aacccgttta
    27841 atggaggctc aagcatgaaa aaaatactgt tgagtgcggc agttctgctc ttgtcacaag
    27901 gcacctatgc ccaaaataac ccaacatcct tatcaaccga cacccgaatc aagaaggtgg
    27961 tttatcggga aaacgatgtg attccagtgc acggcattcc ctttaccacc acgcaaattc
    28021 aatttgcgaa gcaagagcgg gttcttgata ttgaaggtgg tgacaccacc gcatggatgg
    28081 tgacctatca tccacaactg gaaaatatga tttttataaa acccacaatg tttgattcca
    28141 aaactaatat gacggtgatt acgaatcaac gcgcctacta ttttagcctg accagcgcta
    28201 aaaaactgga gcaggagcca ggtaaaaaaa cctatgcctt gaaatttgaa taccccaaac
    28261 cacaacccgt acacacaaaa aatacgactc ccaataaaac ggtgcatcca aaagtggtca
    28321 acaccgctta tcggtttagt ggcagcccgc agcttgttcc tcgacatgta tttgatgacg
    28381 gcaagttcac ctattttgag ctaagtagcc aaggcagtgt tccagccatt tttgcagtcg
    28441 atgaccaaag cggaaaagaa tcaaccgtta atacaaggcg agaaggaaaa tacattgtcg
    28501 ttcagcgttt agccccgcaa tttactttaa ggcaaggtgg ccttacggct tcggttttta
    28561 acgcaccgga aatcaatcgt atcaaagcaa acaggaggcc aaaatgagta tggatgataa
    28621 aaagcgggaa gattctttgg gcaatcggac tcaggttgct aaaaacccaa aacacaacgc
    28681 catgaaggac aagttattgc tcgctagcgt tgtggtgatt tgtattgtca tcggcattgg
    28741 cgggttattc cctaagcaaa aagcaaatac agtaaatgaa ccagatgaaa aacaaagttt
    28801 aagcatggct ttaaaccaaa atctggagct gattgcagca cttaaggaga agaatgccac
    28861 agcacaatcg ggttataaag gcggcgatcc aaggcatccc cccatactaa aagccatgca
    28921 aacacaaacg gttagcaagg aaactctagc tcgaatgaat gctccttcta ccttttttaa
    28981 ttcaggaggt aatgaggtga taagtcaaac aacgggtggc gaagtccaag ccagcaaaac
    29041 cctgacaggc cgtgatggaa attctgagtt tttgaatcag caaaatgata ttacgtctgt
    29101 ttctgcaaaa cgattaccac atcctgccat gaccgtaccc gccggagaaa tgattcctgc
    29161 aacactcgaa acggccatta attcggaact ggcgggtatg gtacgagcca ttacgacaag
    29221 ggatatcttt gccttggaag gcagcagatt actaatccct aaaggctcca cgttagttgg
    29281 acaatttaat accgcaatta cccaaggcca gagccgaatc tttgtcgtat ggaatcgtct
    29341 gcagatgacc aacggcgtga ttgtgacctt aaatagcccc ggcagtgatc ctataggtcg
    29401 ctcaggacaa gcagccgatt acattgaccg tcatttcttc gagcgctttg gcagcggtgc
    29461 gcttttgtcg gttttgggtg cctatacagc aacaggtggg gttaatggac aggatgaata
    29521 caactccaga tcacaatacc gcatgaatat tgccagcagt ttccaacagg ccgcaaatca
    29581 aacattacag caagacatgc aaacacgccc gaccttgcaa atcaatcagg gagctgccat
    29641 taacgtattt gtagctcacg atcttgattt ctaccgtgtg gctgggaggg catgatatgg
    29701 aagccatcaa agcctgcttc tcacctggtg caacaggtct gctctcgcca ttgcaggatt
    29761 ttctggaaga tgagcacgtc tctgaaattt taatcaataa gcctcaagag gtttttatcg
    29821 agcgacacgg gcaattaatt cgatttgata ttccggtatt aactacccaa tacctgcgcc
    29881 gtttgttttt actgattgcc aatgaaaata agcaaacgct ttcggaagcc tcgcccgtgc
    29941 tttctggaaa tctggctgat ggctcaaggg ttcaactggt gatgccacca gcctccctac
    30001 atgaaacact atccatccga aaatttactt taaaacaggt tagcttcgaa gattacaaag
    30061 ccaaaggatt tttttcatcg gcgcgtgggg taggagttaa agagtgctcg gagtgcattg
    30121 gtgaaaccga tgggcttctt gaactctacg atgctaaaaa ctggcaggaa tttataagac
    30181 aagccatttt acagaagaaa aacatcatca tctccggtgg aacctcttca ggtaaaacga
    30241 cttttttaaa cagttgtgta gggcaaatac cgcttcatga acgcctcatt acacttgagg
    30301 atacttatga aatggattta ccccacagca atattgttcg tctgaaagca ttaaagcaaa
    30361 tcggggatca cgcacagaag atcaccatgc aggatttggt tcaagccacc ttaagactac
    30421 gaccagaccg cattattatg ggtgaaattc gcgggcgaga actctttgat tttgtatcag
    30481 cctgctccac tgggcatagc ggtgcacttg caaccattca tgccaataac ccaaaagtgg
    30541 ccttcatgcg catggcacaa ctctacaaac tcaatcaggt tgcaggcatg gatgaagctg
    30601 atatttacaa ggtgttgcat gaagtcattg atgtaatcgt ccagttacaa aaaacgactg
    30661 acggcagacg tttggtggag gtgtattata aacaggcgta atccatgggt tgttaccatt
    30721 accttgatag gtgggcttgc tataaccggc caaatggcag tgctgttaac cggtatcatt
    30781 caatgggcaa gtccgttagt gctggttgat ttactaacac acggatttta ttgggagact
    30841 accatagcag gttgtatcac cgctcttttg atgagttgtg tgcttggctt tttaaaacaa
    30901 ggattttatc gatacctctc ctttctaatc agcagcattt tgctgggtgg aatggctatt
    30961 tttctattgg gatttgaact ttatagctgg atttttttaa gttctgtaca aagcccctca
    31021 ggactcatgg agcttgcctc ccttcgttat caagagcctg agctatggca tcaattcatg
    31081 acagttgaag gtgttgtctt tctcatgatg ttgttagcta ttgcgcttta cctatttcat
    31141 tggctcagac cagacaacaa ggcactgggt aacgcgcatt tttcaaatgg ttttgaaacc
    31201 aaaaaggctg gattttttga gcgtcaggaa caaagtatca ttattggcaa aaaatacgct
    31261 gcgccacttt actccaacgg ctttgaacac gttctggttt ttgcgcccac cggaagcggt
    31321 aaaacacgaa gtattgggat acctaatctg tttcactacc cctattctat ggtgtgtaat
    31381 gatgtaaaac tcacgctttt taagaccaca tcaggctacc gtgaacgtgt cttggggcat
    31441 cgatgctttt gctgggcacc agccgatgag aaccgaatca ctcactgtta caatccctta
    31501 tccttaattt ctgaagacaa aattcaacgc ctgaccgaca tccagcgcat agcccacatt
    31561 ttaatgccag acaataaaaa atccgacccc atttggcagc aagcctcacg caaattattc
    31621 aaagtggccg tgctgtacct gctggacaca ccggacagac ccaccacact tggcgaaata
    31681 aaccgcttgg tcaaacaggc tggatttgat gattggctgg cctctgtctt ggaagaaacc
    31741 gatcatctcg acccagagtt ttatcgcaac ggatactctt atctgaataa tcatgaaaaa
    31801 acgagaagct caattcttga aaccttttcg ggatattttg aattattcga tgatccaacc
    31861 attgacgcag ccacagcacg ttctgatttt gatctgcgcc agttaagacg agaaaaaata
    31921 acaatttaca taggctttac cgatgacgac atggagcggc tctcaccatt gctaacactg
    31981 ttttggcagc agcttatttc cgtcatgatt aaaaacattc ccgacccagt agatgaaccc
    32041 tatcctctgt tatgcctgat tgatgaattt tcatcattag gccgcatcga aaggctaaga
    32101 cgttcactaa aattgctacg cgaataccgt gtgcgttgtg tactcatcat gcaatacatc
    32161 gcccaaacct atgaacaata cacccatgat gaagccaaag cctttaccaa cattaaaacc
    32221 aaaatagctt ttgccaccga ggacatccac gatgcagagt atgtcagtaa attattaggt
    32281 acgcgcaccg tgaaagtatc cgcaggctcc accagtaccc aaacccaagg ctattccgag
    32341 tctaaaagct ataactatca ggccatccct ttattaagac cggatgaagt catgcgttta
    32401 cccgaagatc aaaccctgat tatgcgaaca ggtcatgcac ccgttaaggc ttcgcaaatg
    32461 atttggtatc tggatcctga gatgaagcat ttggcctgcg gtcataccga agtaccgtgc
    32521 caaatcgttc agcatcatcc atttatccat cgttcatcag atagaacggc actgcccgaa
    32581 gcactcgaac tataaaccac cacaggagga aaccaagcca gccaacaaaa ctaaaaggat
    32641 gctctgaaaa gagattatgg attcatacag acttaattga tgtgagaatc agaatgaacc
    32701 atttaaaaat tatcggtaca gcaggtcttt tcatgatgtc atgcgcagct caggcaaatg
    32761 acaaggagct ttgggctcat attgaggctt tatatagcaa aattggtgct ttgcaaacgc
    32821 aaataaactc gataccccaa ggtgctcccg gtccaatggg tccagcaggc cctaaaggag
    32881 agcaaggccc cgccggaacc tacaaagcag gcgaaggcat tgaaatcagt aatggtgaaa
    32941 taagaaccac actgagtcac cacattggtg aagaatacaa cggaggcatt gtcttctggg
    33001 ttgatgaaac aggacagcat ggtttaatcg cttctaaaac cgacctcaat aataacgaag
    33061 ggatacaatg gcgcaacggc gagagtggaa ataaagttac caatgcccgt gcagatggga
    33121 tttatgcagg tgagtccaat accaaactca tcattgccca acaaaccata gacaatcaat
    33181 caggtagctt tgccgcactt ctggctgcaa attatcgcgt gctaggcgat ggcatcacgc
    33241 cttgtccaac ccccactcca gaatcagcca cttgttacgg gggatggtat ttaccttcgg
    33301 cctatgaatt gaccttgctt cacaataatc tgcaacgtca gggaattagc acttttgcgc
    33361 cagactatta ttggagttcg actgaatctg gtgtgggtga ggcatggctt cagaattttt
    33421 cgactgggga gttaacagct agtggcaagg ctagtacttt ggggcatgtc agggcggtta
    33481 gtaggtttta atatcataga ttaactaccc atttatctat gggtagttaa tctatgaagc
    33541 tggttaactt ataatccatt gatattaggt tcaaaatcca gtgagccaac taataaaatc
    33601 aaagttatgg taattttgag accgcagaca atttattttg gattttcaat atatttcaat
    33661 aagctcttac ctaatgcagt tattttaaac cccgagggtt tcatctgccc tgttatacta
    33721 tcaaactctg gaattcctaa ctctctgtca gttttatagt gagcctctat tagacctaat
    33781 tgattcaggt gttgaatgtg actttcatga attgcaaagg tttcaatctg ctctggtgtt
    33841 gatgcaaaag atggcgggat gttttttaaa atatctttgt gtattgagta atattctgag
    33901 ttaccctgat ttggataata aaagccaccc aacttaacta tttctggatc agttaactga
    33961 tttaaaagaa aaagatatcg tttaaacttt tcatgctcta tttcctctcc tatcatacca
    34021 ttagacaata actgagcgat tttctctatt ctttcatcac ttgtagcttt tgttgactgc
    34081 atagtagcat cttctaataa gtcaataaat ttaggatctt taaaaatagc ttctatttta
    34141 ctttgttcaa gacatttaac cttatctgaa agtattttaa taaatttcgt aactctatcc
    34201 attcgttgat ttggaatgtg aatgcaaaaa agctctgcaa ccaacgaccc tatacaaggg
    34261 atggcgctca aactaccttt gatagcagat acaacaatgt cagttttatt attttctaaa
    34321 tccatctaaa tactccggtt atttactcat ctcagttagc tttaaaaaag ctagttacct
    34381 cataacattt ctgtaagggg atcttcttta tatttcggat gtccatctaa tttctttaac
    34441 ggtagtatat ctacgtgtaa atcttatgac actgccctct aaaaaatcgc aggaatattg
    34501 acaaagcgtg gggcagatgt gacattttta gatttgaagc atcaacgctc atgaaaaata
    34561 gcgttttctc tctgcttgtt atagatactt tgtattttta gataaaactc gggatgtttg
    34621 tttttgactt gattggcaaa ggtcttatta tttaatagtt gctgcgaaat tgttgtaatt
    34681 tgtctatcaa ctttttcagc aaagtaacct gtcatttgct gtcgcttctc aataagggtt
    34741 tcaatctcag caatttgagg aaagtccttt tttaaacctt caagggcttg atctgatatt
    34801 ttgtaatttt tggactcaat cacttttgta tcaactttaa atgtttcctg cctcgcataa
    34861 attcttgctc gttctattga atgcaactga tcctgttgcc cagctttcaa aagataagcc
    34921 tcgtaatgta caacgtagtt aaacgcattt ttgattttat gccccacgcc agcgatgtga
    34981 ttaagcacac gacctatgag ctgatccgga ttaaatccgg cacgggttgc aaagtccagc
    35041 ggccaatcaa tcacattatc tcgtgtgtta ctgcgcgata aggtttgttt cagtacttta
    35101 atggtgggat ggttaataga atccgcataa attttgaggc tgtctttatg gcgggtcatg
    35161 gccacaaagc tcaagtgccg atcccagtat ttgctatcaa tcaaaacctt tgaatgtttg
    35221 acagtcatcc cctgtgattt atgaaccgtc agggcataac cgtaatccat tttagtgtaa
    35281 ctacagggaa tggtgagcag ctcgccgctg tcgagtttga tttgcaactg gttgcttttg
    35341 atagattgaa cggtgcctaa atccccgttt cttaccccaa gagttttatc attttgccgg
    35401 aatagcagcc gctccccgat ggctattttt aacggttttt caaacccttg aaaagtgatt
    35461 tcttcctgtc caagtatttg cttctgcttt aatgccaatc gagcctgctc atttaaatag
    35521 ctaacagaag ctctggtaaa tgccaacacg accgaatctt gtaaatttgt attggtaata
    35581 tcagcttgcc actcctggat taagttctgg gctgcggtct cagtcgtttc agaaaagaca
    35641 attgctccct tttcatggta atggtctacc gcctcagcaa tcatgccctt agccatattc
    35701 atactggcaa gcctgtcgcc caaatcattt tgacgtttaa tgtgttcaag ctcgatatag
    35761 cctgttatgg cggctatacc cctaaaaatt tcacccttgt gaatgggttt taactgatcc
    35821 ggatcgccta caagaatcac cttggaacct gcctttttgg cttcacgaat cagcagtgcc
    35881 atgctggcaa aatccaccat gccagcctca tcgataatca aaacatcgtt gctgttgagc
    35941 tttagttgct ggttcgccaa tttataagtc aatgaggcaa tggtggagga tgcaatacct
    36001 gtatccgttt gcaaggattt agcgaccttt ccagacaagg cagcaccgat aacacgatag
    36061 ttgtgctgct cataatgctc cttaatcggc tttaacaaat aacttttacc tgtacccgga
    36121 cgacctatca cgactgaaat ttgagagcct tcggtaatgt agcgaacagc ctccaattgc
    36181 tcatcactca aggtgtagtt tttgatgatg ttgtcgatgc ccttatcgcc aataacatcc
    36241 ttgtttttca tcatggcttc gatatcatcc cgaagctttg cctcctgtat gtattggtga
    36301 cgggtggtat aacacaaatg accccgttca ttgtttccca gctcaatgag atctttatgc
    36361 cccatgattt gctcaaccca gtgcaaataa ttctgagggg tatcacttgg ctgaaaggtt
    36421 ttaaatacca atcgctctac gtcacggcgg gtaaacacgc tgtgctttgc cgaaatatgg
    36481 ttgatgacgt tatcaatatc attcagaaaa acctcctgcc gtgcctgttg aagaatggtt
    36541 ttctcagtga gtagataatg attgtctgca tctttaagtt tgccatgatg ccgttctgat
    36601 atgaggtgat tcaggtcaac cgtcaaatcg agattttttt caagaaaata ctcattttgc
    36661 atatcacgcc attgctctcc ccaatagtct ttttcgacaa taaatccttt ggcaaaagcc
    36721 ggattcagat cgcgtgcttt gtattttgaa aaccctgttt tttccaagcg gcgggtggta
    36781 atcagaatat gcgcatgagg atttccatca tggtgatcat gaattgcaac atcggcagga
    36841 actcccttat caacaaaatg agtctgcgca aatcgtcttg ccagctcaat ctgttgcact
    36901 aaatccagtt ctttgggaag tgccaaaacg atgtccttac acacttgagc atcacatctt
    36961 ttttcagcaa gctctgcctg attccataaa aattcacggt cggaaaatgc ttcaggactc
    37021 ccttcgggta acaggatgtc ggagtaaatg acatcgtgac gattggaata atcatgtgtt
    37081 acgccagtgc gcgcatcata taacttggta ccagaacgat agctactggc agcaatcgca
    37141 ctgtggcctt tggcacgact atgaatggag acttgggcaa aagcaatcgc catgaattca
    37201 cgctcgaatc gaactgatcc atcggttgct ttaataaaag aaaaacacct tatccataaa
    37261 ggcattttgg gtatgcagtt ctaatttagc aaacattcaa atgaatgtat attgcgccct
    37321 tgaattcttc tgcgggatag ccagtgttcg cttcgctcac ttcagatggc acaccctatt
    37381 gattttacag atctggcatt gtacgcttgt tctgttacct catttgcgga gaaatgatta
    37441 tgtcaacgaa gacagtatcg caaatggaag cagtgctctt ggatagaatc gaaaaagcca
    37501 aaatcaagct ggataaattg cagcaaaagc acaagcttga gattggtagt cttgcctaca
    37561 aacatggcct tcaccaattt gatattcagc gattagatgc agcgttttta aagctggctg
    37621 gggagctgcg caatgacaat gcttgaagag attgaaaagg aaaaacagtt gattgctcgg
    37681 tgtgagaggt ctttggcttt ggagaaacta aaaaaacgtc gtgcagatac aagacataaa
    37741 attgaacttg ggggcttagt tataaaatct ggtctaaata tatttagtaa ggcagtcata
    37801 cttggcggtt tagattatat atttgaaatg gcgaagaaca atactagcta tttgcactta
    37861 tttgaatcca gaggcaaaga attattcaat ataacttcag ataattgaag taatgctttt
    37921 ctcatagaat tgggagttgg attgattaaa tttactttag atcaggcttt agatgaaatt
    37981 gcatacagat gctttcgtga tatggcagac caagattata ttttggcgag ggtatcatat
    38041 agattaaact taccgactaa ttttaattgg tgctcattac aagccattga gaaatattta
    38101 aagtgcatac tgttattgca tcggattgag gcaaaagcag taaaccatga tttagagaag
    38161 gcattagaaa aaattaaatc agtaaaaata tcggactcct catcgcagat tgaattccaa
    38221 gatagctcaa ttgagtttat taaagaaatt aacatgcgtg gacaatcaag gtatttaggt
    38281 aaatcaatat atgcaatacc atcagacata actaggttgg ataattgtat ttttgatatc
    38341 cggcgttttg cgataaaaaa aattgatcag atttatcttg aaaaaaccag agatagcaaa
    38401 ctgacccact ttagcgattt ttgtcttgat aatgggtttc ttgaagcagt tgtttccaga
    38461 aatcaacctg caaaagatga cttgattttt aataattcta attatggaaa tttcggatta
    38521 aatatggggt tttctgctca aaatatgcct gccagttata tgggtatgag taatgatgag
    38581 tatatagagc tgtatcagaa attaaacact ttaatcaagg ttgactcttt caaagaaata
    38641 atagcccaat tgaaaaataa caactaatta ctagaaaaat gctatttttt caggttgttc
    38701 ttaatttttt agcctcaata aataactgat tgcattcctc taccatcacc ccagtaatca
    38761 catgacagct ataaaaatct ggtgtcttag acaaaatatc ttccgcccca acctgaattt
    38821 gtcgcccaaa caactcatgc aaaaatgaaa tagaactccc ataaactacc tttggaatca
    38881 tcgcccaata aattgcagca gcacacatgg ggcaaggttc ggcagtggta taaagactta
    38941 acttgcttac atcaccatcg aatccattct caaagagtct atgaataact gataattcac
    39001 catgcatcaa tggattgtta tgtgcactgt ttaccgactt caaaaggatt tccttatcat
    39061 cgtaaacaat aatcgcacca tagggagcca tagggttagt aacagcaact tcaatggtgg
    39121 cagccataaa gtcataaata tatttatcca tcgttacgct ccgctggttt catatagttt
    39181 tcagcaaact tgttcgcgct gaccttgtaa ttggtatcaa ttcggaatgg gtttaaattg
    39241 gatagctcgt cataaatatc aaccttctcc ttcactttga ggaaatatat catcaggatg
    39301 gacactggcg cgagaaagac ctcatacgat attttatagg catcctcaaa aacaatgacg
    39361 ctagccagtt tttcagttgg aacaatccca tagaacgcca ccaatgaaaa caagctaaca
    39421 tcgataatga ttccaaatgc cgatgaggtg gcgattcgag tccaaacggt ggtgataaat
    39481 tcctttttca gggaaatacc ccgtttgcgt tgtctggatt tgatgagtga aataacggtg
    39541 gaattgatgg tcatacccag aaagaaggcg gtggcagaag caatgaacat tcgcgcctga
    39601 ttgtgcagta tcgctcggta cggttcgttc tctccaatcg ccagcatatt ggttacccaa
    39661 acatcaaaaa caaacagtag attgcaaaaa ataacaaata gcaccatctg cttggtgcgc
    39721 tcaattccat aaacgtccgt catcatatct gcagcaaggt aggccagtga aaagacaata
    39781 cctccaacat tgatggtcaa aaatccaata gaaatgaatt tggcagccat ggtcttggat
    39841 gcgataaaaa agacgatact ggaacaaaac agcatgtgat gatatttgaa tgtcttgcca
    39901 tcgttcttta ggacttgtgg caattgaaat ttaaaaagat gcctgtccga ctggattggg
    39961 ctttctatgt gttcaattct cgcgatcacc acagagagcc aattataaaa aatattaagc
    40021 aacccaaaga tgagcaaagg aaccacgatc tgggtgtcgg gggtattttt gtgcatgttt
    40081 aaccagccca tatccaaaaa gcaaaacgca accaaagata cccagaagat atacccgtaa
    40141 tatttatgat ttgccgaact cgcaaaacgt atcgggagaa gcgcacatcc ccataaaaat
    40201 aaagaacttt ttatcaggta tagaaaatcc cgacccagca ccagcgtgta aggttcttgc
    40261 attttccaga atgaagaggc aggcagccaa atggcaaact tcaagaacaa aacgtaaata
    40321 gcatgaaata acaatactgc atacacagca tgtctgagat atgcgaagcc ataaacacca
    40381 tgaataagac gcaacacaaa aaagacgact ggccaccaca gtaattctga ggatgtgagt
    40441 attccatgga tttggatcag ctgtgcttct gctggcacgg taatcaaaga aaagaagact
    40501 gcaaaaccca atagcgtatg gaatacttta tactgcaagg gagttttgga tttgcctata
    40561 atggaattat tcatcgataa aaaccctctt gagggtggtg acgcatttcc tctttaaact
    40621 gggttccgtc tttttggcaa cgatcagttt ttcagaatga gagccaaact gctgccccat
    40681 atcaaacacg atgcgagggt caaccagata aatcatatcg gttttaacaa actcagccac
    40741 agacatatcc tgattcacac gcttgttggc aatatgaaag gcaacggtta attcattgct
    40801 gtcgggatcg gtatagatat tatcaatgcg aataagataa ggttccctga tttgcccttt
    40861 gaacctcaaa tacaaatgct gatacaaaaa ccgtaaaaat cgatttcctt gattccactt
    40921 ttccatgttt acatcctaca acttattttt tggagcgtag cttttgatgc acctcaagaa
    40981 tcacaccagc gacagtgtat aaatttctat cgaataattc tactggtaat ttaggattga
    41041 tggggtacaa atagccttga tttccttcta aaacgtattt tttaatggaa caatgctggg
    41101 ggctatcatt cctcttcaca acaatgtacg agcgatcatc cggcatcatg tcgggattaa
    41161 caatcagtaa tgagttttgc ggaaacgtag gctcatacaa atcagtatct atcgcgagag
    41221 caaagtaatc accatcaacc tcattggatg accagcgata ccattcgttt gtcatttggg
    41281 ttggatggct gaactcataa ataggtaaag actgcaccaa aggctcgata ttcgatgttc
    41341 cttcacgaac aatggattct aagccgagaa gctgttcaat agtaattcca aaatagtttg
    41401 caatttgcag cagggtgctc gcttttggat cggtaacatc accattcaaa atccttgtga
    41461 ttttgttggt tggtatacct agctttcgtg caagtgctgc aggcgtcagt ttattctcac
    41521 gttcaattaa atccgtaagc gtttgcgcta attggagatt gttagattca cttaaaatat
    41581 ttgtcgtcat accttgactt tcgttacata gatgtaataa tttacattga tgtaacgaat
    41641 tacatcgatg taaaactaat attaccatat tttatgggaa tatcacttag tcattggtca
    41701 tgattttaat taaatcagca gcgcagtaga tttgccttgc agtaaggagt gtttgcatat
    41761 ggatcagcaa gtaaaactga acatctataa gggaatgatt cagtacatca tcaattcgac
    41821 acattatacc ttgaagaaca tcgcggattt gtcggatacg cacatcgaca acattcgttc
    41881 tatttattgt cacaatgctg ttcctaacgg tttcaagtca gagatgcagc tcatgaagct
    41941 ctatcaaatt gttcttgaaa taagttccaa tccaaagcac cactctttgg ctggttagct
    42001 ttcataaata aagaaggaag ttttgattat gaaatgggta aaacttaaaa aatattgtca
    42061 ggatactgga gacacaacta atgcagtgca ctgtaagcgc aagcgtggca tgtggctgga
    42121 tggactacat tgcaagttag gaccggatgg caatttatgg attaatttgg tggaggtaga
    42181 gcgatgggtg gaaaacggcg atcaggcgac actccaaaag ctgcaagcgg ggttatagta
    42241 agaaagtggc cttcaggcaa aacaacacta agaattagct tttattataa aggggtacgt
    42301 tgttttgaaa ccctgaaaat cgaggctact gccgccaata tcaaatatgc agaacgatta
    42361 aggggtgaga ttttaaatgc gattgagcgt ggtaattttt cttaccctga ttacttcccg
    42421 aactcaaaac gcgcccatat ctttgggcat gttcagtcaa aaatcactat cggtgaatta
    42481 ttgcgtgaat ttttagcaga agcaaaatcc acaaaagagg cgagtactta tcgaggttac
    42541 aagcgtgtat gcgaggggca tttattgcct atgtttgata aagtggaaat acaggactta
    42601 caacctgcca tgcttagaaa atggattcga ggattacagt gcactaccaa aacagtggca
    42661 aatattctga cccctctacg tgctgtcatt gaacaagccc tggtagatca atatattaaa
    42721 gaaaatccgc tcaatagcat tattgttgat aagctactta acaaagaaac taaaaaaagt
    42781 gattacaagc ctgatccatt cagtgtggat gaaatcaacg ccatattaaa tgaatcagag
    42841 gggcaagtgc gattactgtt tcaatttgca ttttttacag gattacgcgt atcagaacta
    42901 atcgggttaa gatgggagga cgtagactgg caaaatcaga ttattcatgt tgaggaaacc
    42961 attgtctaca aggaagcaaa agggccaaaa acagaagcag gcgttcgaga tgtcctttta
    43021 ttgcctccag cattagaagc attggaacaa caaaagcaat acactttttc acaaaaagga
    43081 agagtatttc ataacccgca aactaataag ccatgggaaa cttcacagca aattcgccgt
    43141 acccaatgga tgcatatttt aaaaagagca ggtatccgtt atcgcaatac ctaccaaact
    43201 cgacatacat atgcatcaat gatgctttct caaggtgaaa atatcatgtg ggtatccaag
    43261 cagttggggc atgttgatgt tgagatggtg attaaaactt atggtcgttg gataccggat
    43321 aattcaacgc agtcaggata tcgtcctatt catgattggg gtagtcatct aggataatat
    43381 tcatgttacc atagacaatt acttctaatt gctattttgt tatgaataat gctgttgaat
    43441 gtcttgatct agaggctatt aaagccaaat ataaagaaaa tgaagcgcat ttcaatttat
    43501 tgcgtagcac cttaatcagt tgcgctagaa aaattaaaat tttttcaatt atcgcaaccc
    43561 aaaaacatac aaaaactgaa ttgactcatg aagatttttg gggatggcta caaattgaaa
    43621 ccttacttcc taacatcata ttgtcttgct atgagttagc aaaagggcta aaagaataca
    43681 acagcacttt tctaaagccc cttttgccgg atgactcagc taagtcactt acagagaaaa
    43741 gcaaattaca gtatggtatc agtgcattat ataagcaaac taaaaaattt gttaaagtcc
    43801 gaaataaacg catcgctcat cttgaatgtg cagtggttga ttctattact gctgatttgg
    43861 aagcattgta taagtgctta gcgagaattg atttatcatt aagttacata atgcattact
    43921 tgctcaaccc ttacttaatt agtgagcaaa attggagtgc tttcgattta gataaaccac
    43981 aagatttaga ctcactagac gcgtattttg agcatgatcc ttcaatacgg gattgcaaag
    44041 aaatattgat gtgtggtgcc gatatggtgc cccagaatgg ggtgggttga aattaatgct
    44101 tataaatcaa taacttaagt ggtggaggcg gcgggaatcg aacccgcgtc cgcaagtcct
    44161 ctgcctttag ctctacatgc atagccttgt cttttgaatt taactgtggc tcctccgacg
    44221 ggcaggatga tacacagcga ttccctgagt ttcgcgccat aacccgggat agattaggac
    44281 gctagcttat ctcttatgac cgttgattcg ataccgataa gcgggctcgg tcgaacggga
    44341 tactgggttt attggccagt acacagtgat ggtttaacta aagtgcttat taggcagcga
    44401 tagcttcccc accagcaaag ttttcatcgt ttgcatttat atttaattga gtcttattta
    44461 cgagagatct ccttctcggc atgcacttcc ggttttgcaa cccacgtcga agccaggtcg
    44521 ccccccattc tgtacacttt aattataaca taaaatgccg gcgtgtaagc aaaatcccgc
    44581 cagcataccg aataaatttt caggacttac agcccctcaa tgccaaaaca caatagtttt
    44641 taacaacaag attggggggc gtaagccctg attttataaa atcccattat ggcgcagcaa
    44701 agcatcaatc gtcgcgggtc tccctcggaa ttcaacgaaa gcatcggctg cttttcttga
    44761 gccgcctgcc tccaagatgg atttcaaaaa gtcatgcccg gttttggggt tgaaaatacc
    44821 ttcctcctca aagcgcgcaa aggcatcgct ggataatact tctgcccaca tgtaactgta
    44881 ataaccagcg gcgtagccac ccccaaaaat atgcgagaaa ctgtgctgaa aacgattata
    44941 aggaacaatg ggtacaacac tggttttaga acgtacatcc gccagaatat ttgggacaaa
    45001 tgacgcttta cccgtttgat attcctggtg aatgcgaaaa tcaaacaagg cgaattccat
    45061 ttgtcttaac atggccatgg cggattgaaa atttttagcg gcaatcaagc gctcgtacaa
    45121 tgcggaaggc aaggtttccc ctgtatccac gtgcgaagtc agtaaagaca atgcgtgctc
    45181 atcccagcac cagttttcga aaaattggct gggtaattcc accgcatccc attccacgcc
    45241 attaattcct gagccgccaa ggtaatcgac ttgagtcaaa acgtgatgca ggcaatgacc
    45301 aaactcgtga aacaaagtca ccacttcatc gtgcgataac atggcgggtc tgtttgcaga
    45361 aggcttggca aaattgcaag tcaaggtcgc aatgggtaat tgaactgtgc catcctcgag
    45421 ttttctgcgg ctttgcatgg agtccatcca ggcaccattg cgcttgtgcg gtctggcaaa
    45481 caaatccgtg taaatgtacc cgcgcgtttg attggattca tcgactatgc aatagcattg
    45541 aacgtcctta tgccaaacat ccacaccctc aatctcctcg atactcatac caaagagctt
    45601 tttgacaatc gcaaacaaac cttgcatcac tttaggttgt gggaaataag gtcgtaactc
    45661 ctcttgagat aaggaataca aatcttgcct tcttttttca gaaagataag caacgtccca
    45721 tgggtttacg gggcttagat tgaatttatc ctgcgcaaac agttcaagtt gcctaaattc
    45781 agccttaccc ttgtcacgag ttctgctgat caaatcataa ataaattcag tgacttgatt
    45841 ggtcgaggcc gccatcttgg tcgccaggga taactcggca aaattattaa accctaaaag
    45901 ctcggctttt tcatggcgca gggacagaat ttcatcaatc agcggagtat tatcaaaggt
    45961 tcctgcatta gggccttgat ctgaggctct ggtgacgtaa gcccgataca tttcttcacg
    46021 taaagccctg tcttcagcat gagtaatgac ggcctgaaaa caagggtatt cgagtgttaa
    46081 aacaaatcca tccagcccct tctcatgcgc caattctttt gccgtattta aagcgtgctc
    46141 tggtaatccg gccaaacgtt ctgcttcggt gatatgaata gtgtaggcat gggtcgcatc
    46201 cagcacattg ttttcgaatt tgctggataa ttcggccagt ctcgattgaa tggcttcaaa
    46261 acgcttttta ttcgccttgg ataaagcaac tcccgataac tcgaaatcct gtatgctgtc
    46321 tgcaatgatt tttttctggg cagggttaag ggaatgctga tctatcgatt taatcgcctc
    46381 atacaagtct tgattatgcc caatcgcagc gtcataggca gacagcaagg gcaaacatgc
    46441 ctcataggat tcacgtattg cctcagaatc catcactgca tgcatatgtg caaaaggaga
    46501 ccaaaaacgc tccagttcat cagcgagact atctaaaggg taaattaaat tatcccaggt
    46561 atagtggtga ttttccttaa gcagcctgtc aatttcctca agatggtttt ttaataaagc
    46621 atcaagatga gatttgaaat gttcaatttt gatatgacta aattgcggta atccaactgt
    46681 ggtcgacatg ttataccttt ccatcatgta attttttgaa agcataacat tcaattgaat
    46741 tagtctctat actttgccgg cgctgaaatg gcaaagtaca gtaatcaatt aaaggcggat
    46801 gttgggatta actcccccac ttcatcgtct ttttctgtct tggcagaaag atgcaggctc
    46861 tctctttttt catttttgga ggcaaaaaag cctaatttgt tgataacgga aggagctggc
    46921 ttttcaatgt tatcgacagg tctgtattca tctagcttgt tgttcaagta taatgaaacg
    46981 ccccatagcg ctagcaaaag aactgctcca atcaagggga tgacaggatt tataaagcct
    47041 aaagcaggaa ctgataaagc cattgcaatc acaacagcga ctgccccgcc aaccgataaa
    47101 aaggagcgca aaatctcaaa cttaatgcga taactgatcg agtcttgata ctctttaatt
    47161 atctttttct ggtcttcatt ttcttcctgg tcgaagagtt cgccgtattc cttttgcaac
    47221 tgctgtaatc gaccaatctc tatgtaagca cgcaggcttg tgttggcgac gtcaaagaca
    47281 aacgcaaaca acgacaaata aacagagagg ggtgccaaag cgcctaccag cacgaagcaa
    47341 ttgagaatcc cgactgtaaa ccaaacgctg tcattggcca gctcaaacca ccgtcgttgc
    47401 aggtgagcat aaaatcgatc ggaccaatcc agtgattttt ctttttcacc catccattcc
    47461 cctggaatag tgtgctttaa aattaaaaag agattggtaa ataatctggg ggcaaagaaa
    47521 caccagccca aataagccaa aaatggatta gtgtatttat caagcagtac gacaaaatca
    47581 cgaaatgctt ttgaaccagt gtctaaatta tccaaaagat taatgactcg tttacttctg
    47641 ctgataaaaa ttctatacca attggattga gcagttttct cacgaatcgc ctgcgtcaac
    47701 gataagcttg tcttgagaga ttctggctcc ggattatcta aagcaaaaga atacccggac
    47761 aattccgtca ataattccct gtatatggct ttatggcgac gtaaacgcac tacttcgcgt
    47821 ggaacgacca gatagtgttg ataggtatat tccaaaagtt cagccagttt gagtgcatca
    47881 atcagttgtt ccttgatttg ctcgggagaa ctgtaatcag gcgaggacag gttatacctt
    47941 aagcgctggc acaatacgct gtattccata ttgagaatgg gatgaagacg agctactttc
    48001 gaataaaaat cagggcccaa ttgcttcagg tgcgtgttaa aagtccaatt caaagaatca
    48061 gttttaactt gcgtgttcag ctctggaaat atttttaata aatgactaat aatcggtgtc
    48121 atgattgttt gaaataaata atccgcatat cattgtgttt ctaatccagc tttgcaaaaa
    48181 tttttgctcc tgtcacctta tctgggctgc aaaaattaat caaactccat cattatgtca
    48241 aaaattagga aaaatttaaa cttaaattta cattttgctc taatattgtc attattttca
    48301 actaaaaatg aatggtcttc ttgccgattt atgccttctg agcgacaata gtgcctcagt
    48361 taaatgcaaa agagtaggca tgaacagttt tacagaatta gctaatgcag tacgcatgtt
    48421 aagcatcgat gcagtaaacc aggctcaatc aggccaccct ggcatgccct tgggtatggc
    48481 tgatattgcc accgttttat ggaaaaaatt tctaaaattc aaccccaaaa atccgcactg
    48541 gtttaacaga gaccgttttg ttttatctaa tggccatggt tccatgcttc tctattcttt
    48601 actgcatttg accggataca atttggatat taacgagctt aaaaacttcc gccaattgca
    48661 ttcaaaaaca ccaggacatc cggaacatgg gcacactccc ggtgttgaaa ccacaaccgg
    48721 gccgttaggt caaggtttgg ctaattcagt cgggatggcc ttagcagaac gtgtgctggc
    48781 cagtaccttt aaccacgatc actataacct agttgatcat tatacttata cttttgccgg
    48841 cgatggatgc ctgatggaag gcatttctca tgaagcttgt tctttagctg gcactcttgg
    48901 tttgggaaag ctcattgtat tttatgacga caatgggata tctattgatg gcaaagtgga
    48961 atcctggttt acggacgata ccacgtctcg ctttaaagcc tacaactggc aagtcatagg
    49021 ccccatcgat ggccatgatg caaatcaaat tgagcacgcc atattagaag cgagcaagaa
    49081 caacacccaa ccttcattaa ttatctgtaa aaccgtcata ggactgggct cctctgtggc
    49141 aggcagtgaa aaagcgcacg gttctccatt gagtgcccaa gacataaata atgttcgcga
    49201 attttttaat tggaaacatg ctcccttcga aatcccagat tcgatataca agcaatggga
    49261 tcatagagag caaggagaaa aagaagagca acaatggctg cagctcctgc atgaataccg
    49321 gcaacaattt ccatcggaac acgatgagtt tttgcgtcgt gccaatggcg acctgcccga
    49381 tgattggcaa gaaatatcgg ctggattcct aaaacaatgc ctgaacaatg agaaagccat
    49441 agcaacgcgc aaagcgtcgc agcaatgcat agagtttttc gcgccaatat tgccggaaat
    49501 gtttggcggc tcagcggatt tgactggttc caataatacc gattggtcag gcagcaaagc
    49561 catcacgtcc cataattttt ccggaaatta cctttattat ggagtgaggg aatttgccat
    49621 ggcagccatc atgaatggac ttgctgtgca tggaggattc attccctacg gcggaacgtt
    49681 ccttgttttc gcagattatg caagaaatgc catccgttta agcgctttaa tgaaacaaag
    49741 agtgatatac gtattgactc atgattctat cggtttaggg gaagatggcc cgacacacca
    49801 acctatagaa catattgcga tgttaagaat gacccctggc atgacggtct ggcgccctgc
    49861 tgatttgatg gaaaccgctg ttgcatggca acaatcgtta gaacatcata atggtccgtc
    49921 tgccttgttg ctctcaagac aaaatctgcc cgctttagcc catggcaccg atgcggctga
    49981 cttgattaag aaaggcggat atattattgc agattgtgaa gggaaacctg atgctatcct
    50041 gatagccact ggttcagaag ttcaactggc tataaccgca gcagaaaagg caaaagcaag
    50101 aggtctccaa gtcagggtgg tatccatgcc ctgtgctgag cgattccttg cccagagtga
    50161 tgattacaaa aatcagatat tacctggtca ggttcgcaca cgtattgcga tagaagcagc
    50221 tagcagtgcc tattggcacc aatttgttgg ctttgatggc gctgtcattg ggctggattg
    50281 ttttggggta tcagctcctg ctacagatgc ttttaattac cttggaatta cagtagaaaa
    50341 aattatcaat actctagata cattaaccaa acaaacaatg gtgtaaccca taccccttct
    50401 gaagtttcaa gaaggcctgt attcagtata actgagtatg gaggttaccc taactcggag
    50461 aagtagtatg acaatacgag tcgcgattaa tggctatggc cgcattggcc gttgcatttt
    50521 aagatcgatt tttgaataca acaggcaaaa tgagtttcaa gtcgttgcca ttaacgattt
    50581 atctggcatc gatgtgaccg ctcacctgac tcgctacgat tcaactcacg gtccttttcg
    50641 ctcggatgtg agcatagaag gaaacatgct gattgttgat gggcatggca ttcaagtgat
    50701 tgctgagcgc gatccggcca aactgccatg gaaacaattg gacattgata tcgtctttga
    50761 atgcaccggt ttctttacca gcagagatgc ggcaatgggt catatcaatg ccggcgccaa
    50821 aaaagtatta atatctgccc cggccaaagg cgttgatacg acagtggtct atggagttaa
    50881 tcactccgtc atcaaagcgt ctgatgtcat tgtgtctaac gcttcctgta caacaaactg
    50941 cctggcgcca gtagttaaag tgttacatga tgccatagga attgaatcag gattggtcaa
    51001 cactgtccat gcttatacaa aagatcaaat gcttttggat ggaagccata aagatttacg
    51061 gcgcgcccgc tctgcaacgc agtccatcat tccaaccaaa accggcgctg ccgcagccgt
    51121 tggattggtt ttacctgaac tggcagggaa attggacgga tttgcgatgc gagtaccggt
    51181 attaaacgta tccgttgttg atttaacttt ccaggcaaaa cgcgcaactt ctgtggaaga
    51241 agtcaatgac atcatgcgca aggctaaaag ccgaatttta caaattaatg aggacccttt
    51301 agtttcttgt gacttcaatc acaaccccgc ttccgctatt ttcgacacaa cagaaaccaa
    51361 agtgataggc aatttggtca aagtagtagc ctggtatgac aatgaatggg gtttctctaa
    51421 ccgcatgatt gataccgcac accaaatgat gaactgctaa aggataacca atgaacctga
    51481 ttaaaatgag cgacattgat ctgtctggca aaagagttct gattcgtgag gatttaaatg
    51541 tcccgatcaa agacggcatg attaccagtg atcaaagatt gcaagcggct ctgcctacaa
    51601 tcaaatccgc tttggacagt ggcgcagcag taatcgtatt gtctcatctt gggcgtccgg
    51661 aggaaggcaa atacgaaaaa aaattctctt tggaacctgt tgctgattat ttaagggaaa
    51721 acctggaata tcctgttcgc ttcgtcaaag attacctgag cggcgtggat gtaaaccctg
    51781 gggaattggt cgtatgcgaa aatgtccgat ttaatcctgg tgaaaaaggc aatgacgaag
    51841 ctttggcaaa aaaactggct agcctttgtg atgtctttgt catggatgct tttggcacgg
    51901 cacacagggc acaagcttct acctatggtg tcgcccaata tgctccagtt gccgttgctg
    51961 gtcctctgct aatcagggaa cttgaagcgt taaaccaggt gctcaaagcc cccaaaaaac
    52021 ctatcgtcgc cattgttggg ggagccaaag tctcatccaa gctcagttta ttgaaacaat
    52081 tagtgggtat ggtcgatgtt cttatccctg gtggaggtat cgccaatact tttctgaaag
    52141 cccagggatt tgaaattggt atttcccttt acgaacctga tttattggat gaggctcgcc
    52201 atatccttat tctggccaaa gagaaaggtt gccaaattcc tctacccacc gacgttgtag
    52261 taggcaaaac ctttagtgaa acctgccctg ctttcaacaa atctctgtct aatgtggctg
    52321 cggacgatat gatccttgat attggaccag aaaccataag agattatgta gacctcatcc
    52381 atgaagccaa taccatcatc tggaatggtc cggttggcgt attcgaattc ccgcagtttg
    52441 cttacggaac aagagccatt gctatagcga ttgctgagag taatgccttt tctattgctg
    52501 gtggtggtga taccttggct gctgttgacc tttatgactt gaatcaacaa atttcttata
    52561 tttccacagg aggtggggca tttctggaat gcctggaagg aaaaaccttg cctgctgttg
    52621 ctattttgca ggagcgcgca aaacatgtta agacgaacta agattgttgc cactttaggc
    52681 ccagccagca aggaacctga aattctgcga tccatgctgg ctgctggagt caacgtagta
    52741 cgaattaatt tttctcatgc ggattcctcc gccctgcaat taattgcact ggttcgtaaa
    52801 atcgcagatg aattaaacca cccggttgca gtcatggctg acttacaagg gcctaaaata
    52861 agagtcggcc gttttcaaaa caaatccatc actttaattg atggacaaaa ttttacattg
    52921 gattgtatgg ccccagatac tctgggagac ataaatggcg tatcggtcgc ttatcctaat
    52981 ttggccaatg aactcagtat tggtgatcat cttctgatta atgatgggtt aattgaattg
    53041 gaagttatag agatttccgg gtcaaaaatt cattgcaagg tagtggaagg tggagtactc
    53101 actgatttga aaggtttgaa tcgaaaaggt ggcgggttag cagccagaac tttaacagaa
    53161 aaagatagaa atgatttacg tacagcaatt gaagcagaag tggactacat tagtttatct
    53221 tttgtaaaag acgcagagga tatccggcaa gcaagagcgc taatgaaaga ctatggggcc
    53281 caaataaccc ctattatcgc taaaattgag cgcatggaag ctttggatca cttaactgat
    53341 attattcgcg aagcagacgc catcatggtg gccagaggag atttaggcgt tgaagtgggt
    53401 gctgctgaag ttcccgctat tcaaaaacac atcatagaac aaacgcgact tttagataaa
    53461 gtggtgatta ccgcaacaca aatgatggaa tccatgataa gtaaccctca gcctacccgt
    53521 gccgaggttt ctgatgtggc caacgcgatt ctggacggaa ctgatgcagt gatgctttcg
    53581 gctgaaacag caagcggcct ttttccagtc aaagtcatta ccatggtcaa taaaatatgt
    53641 ttaagcgctg agaagcatgc cagctttttt tatcatagcg acccggaaac ctgccattat
    53701 caacgtgccg atcaggcaat cgctatggct accatgcata ctgccaatca ttttcccatt
    53761 caggcgatta tcacactgac cgaatccggc gatacggctt tatggatatc ccgacagcac
    53821 agcactgtac ctatttttgc catatcagcc aacaagagaa ccattggccg attaagcctc
    53881 gtaaataatg tctttcctat ttacattgat tttcatcaat tcaatcctga aggattgaat
    53941 caacaaattt tgcatgaatt agtcaaatca ggacaccttg aaaagaaagg ctatgtgtta
    54001 ttaacacgtg gaacccaaat tggaactcca ggaggcacta actgcatgga aattatacct
    54061 gtcgtttgag tgcctggcat cttgttagtt aaagcagcgt tctttcataa acgataaaaa
    54121 tgattatttt aatggcactt taatcaaact atcacctcta tgctactatt aagcatagag
    54181 gtgataaata tgccagatca taactcatca gatattccgt tatcgaagca aataaatgaa
    54241 gtcagggggt tgctccttaa ggcccttaaa gcgcaattgg atgatctgat agctgaaaaa
    54301 ctcactccac aaccaagtgg cgttcctttt gttcattttg agaaggatcc accacaaatt
    54361 gcaactctaa aaaaagtcat taattgtctt tatcatgctg aggaagcatt taaaagctgg
    54421 gaaagcattg ataccagtac gctattgggt aaagccaaag cagcgcccaa attaatccgt
    54481 gcactaaccc agatatacaa atccctgggt ttacttgatg aagctacccc tgaaataagg
    54541 agtgtcatag ccgacaatta tcatttactt gaaccgctgt ttactcaagc ttataccata
    54601 gtgcaagagt ctggctggtt atcagaattc atggaaatgg atgtgactga taaagcctcc
    54661 aaagtgatct ttcaaggaat ggatttacta ggtcctgata taggcaaatc agaggatacg
    54721 catccactta ttgacgcttt ttcaaaaata tcaaaactgg tagaaaccgt atccacacta
    54781 caagaaaaga gcatgacagg aaaagagaaa gaacgagccg tagaattggt tcgttcttta
    54841 cttgatgact tggacaataa tccttttatc agtaaattaa gtattagtga ttttgaagac
    54901 tcaaaagcca taaaggattt attagaatgg tttaagaaca tacaagacga tggttttgat
    54961 tttagcaaga aatctattca gcaatatgtt tcctgggcta atcactattt acccacatta
    55021 atcacctttg ctgatcaact ggaacgacaa aactatttaa aaccaggcag tttaagtacc
    55081 aatttgtgtt ctcaaataga gcatttaggc aagcaagtga atggcatact atctgaacct
    55141 aattttggta ttactgagcg cgttgtatcc atagaatcat tgaaggggaa aagagagcaa
    55201 caaattcata atgctcaggc caacagtgtg cgagctatca tggctaccga aaaacagcaa
    55261 gcggctactg ctgattttta tcggatcctg gaagtctata aaggcatgaa tctttcacaa
    55321 gtcacagagc cggatcggat attactaaga caattatatc cccaaataca aacagcactg
    55381 gctcatgcca gcttggacct tgaaaatctg ttaacctctg ttttaaatac taccggccct
    55441 gaaaaaaccg cacctaaacc caaatcatgg tgggaattag ccaaagatgg tttaggttat
    55501 gtagccagtt atgtagtaaa ttacaacgtt gataaaatcc tgggaaccag agaatctgtt
    55561 gatcgttttc tgtcaagcca aattgcttcc gaacaattta agatcctgat tgctgaaaaa
    55621 gctcgtgaaa aattaaggac cgggtcagat aagctagaag aaaccgcact gcaatataaa
    55681 gccgaagttc gtgtcaatac tataaaatca accctgagaa agcaaccttc tccgatagag
    55741 tctcctccat ccacatttgt tgcagtgaaa ccttcgaaat tagtcaatct tcgtggaaca
    55801 ctaactgcca ttcaggaaat gcaattatcg actacagtaa acagcacaag aacaaatgtc
    55861 gatactctga ttcatcgcca tttgccagaa aaatatcaag cctgtttttc aactcttccg
    55921 tatcaaatca atgaaaagga tcctgatttg gtgaaacaaa ttaaaaccgt tgaaaataat
    55981 ttgtttcaat tagaaaaagc gcttaaagat tttgaaaaaa tcgatttgag ctatgggatt
    56041 atgatccgat tgcattattt tatcgcaatc gcttctgcag cttccaaact aaaatcaagt
    56101 gtcaaagaac tttcaccaga gggtcaaaaa gcgctggctc cactgttgca acaaattatg
    56161 actttagggt catctttctc agacaccaat tatacagcta atgacttatc cgccttgcaa
    56221 caattaaaaa cagaaggcca agccaaaccc agaggtccac tcgaaacatc tccggaaaaa
    56281 acaaaaacat tacctgaaga aactgtaaag ggaaatgaaa aaaaaccaaa agaaaaaacg
    56341 actgaattat tggattatgc tgataaaatc agtactgctc ggacattgtt attaagtaag
    56401 tttaaatcca ccttatctcc tgccatagct gacagtctaa acccacagga acatggcgtt
    56461 ccatttgttc atcttgatca agacccgcca caaatcgcgg caataaaaaa aatcatcaac
    56521 agtatgtatc atgcagagtg tgctcttaag acctggcatg gcatagacac cagcactaca
    56581 ttgggtaaga ttgctgcagc acatcaaggc gtagcggcat tatcgcaagt gtacaaatca
    56641 tttacgttgt ttactgagga tttctcagac attcacaatt taattcgtga gaatcacgat
    56701 ttaatagcac ctgtgatcat cgaagcaaac aagctggtta aagagtatgg atgggcaagt
    56761 caattcagtg aacttaatgt agcgcaaaag ctgggaagca ttttaggtca gggaattaat
    56821 actgttcaac ctaaaacgga tgaaataaca caaaccgctt cactagttaa attattttca
    56881 gaaattccac ggctactgaa caatatgtca agttcattgg atcaaaacgc tgagaaatca
    56941 gttgatgagt taagaatcaa cagcaaaaaa attgaagcca taggtgcagt atttgaattg
    57001 ttctttgaag aaaatgctta cttgttgagt atttttaaag gattcagcgc catctctggt
    57061 cttcttgaat taaataaaaa aattcaacgc gaaggaaaca acctgcaaga agtcactatt
    57121 cgacaatacc agaaatggtt aaaagaaggt tatcccaatt tgatgatgat gcttgatgaa
    57181 attgagacac gtcactactt aaccccaggc cttttatcag cacctatagc cttggaatta
    57241 gaccaaatta acgacaaact caatgaaatt attgaaacca agcctgattt taaattgcaa
    57301 aaaattcctc tttcattcta tatgggcgac aagcgcatgg agcgcttaat agctaaaaaa
    57361 acggaacact gcctcgcatt gatccaaata gaagagcaaa aaaaagcggt taacactttt
    57421 ttcgggatac ttaaacacta tgcaggtcaa ccactctcca acataaagcc aaatgagctc
    57481 gttcaattgc gtcttgcttt tgtaaaaatt caatccgcaa tggcaaatag taatttggaa
    57541 ttaagtaatg atttggttgc tgctttaaat caacttgaaa cgtccccacc tgaaaaaaca
    57601 aagaaaatca aggtgacagt ctctcaattg ataaaaatgc agcctgctgt tgaaaacaat
    57661 ttaaacgaac acaaaaaatc ctgtgaatta aaaattgcag tcattgataa agccttagat
    57721 cacgttatag ccaaatcttt cgagttaaaa tctgatgtat caaaagaaat caatatgccc
    57781 caattgcgca gaacattttt agaaatcgaa ggcaagaaac ctgctatagg ccctggagaa
    57841 ttaaagccaa catcggtctc atctcttaac accgttcgtg gtaatttagc ttacattcaa
    57901 gaattaaaat tgtcatccac tgtgggtatg atacgagagc aattttcggc tcttactgga
    57961 gataatttct ccactcgagt gcaaaaatat ttaatcaaac cccaaggcaa accgttgcat
    58021 gtgataaatg acaaagatcc actcatggta aggcaaatta aagaagtcga aaacggatta
    58081 tatcacctgg aaaccgcttt aaaacaattc gagcaaatga aaaagagcga cagccttgta
    58141 tcgcaaacaa aagcgttggt agaaattgca aaccatgctc atgaattaaa aatggcagtg
    58201 gaacgatttt cacccgagtt aaaacagcat tatggcccag tagttcgtgc cgcattgagc
    58261 ttcagtaata aagttcaatc catcaattac aacaaagagg attgggctga tcttcagttt
    58321 attcttaata gagcccataa agaattgtta aaaagaaaaa caccaagaga ccatcgtatt
    58381 gaaaaggcat tttttgaagc tggaattcgt gaagtacaaa tcgatcggac tgaatcagta
    58441 ggaaaaaaag cggcaaaact gggtgtaaaa tacgcgcacc tggcttctcc ccagttagag
    58501 aaagcaagaa gctatttaag ttccagatac aaaaatgcgt ttggccaaca acccaagctt
    58561 attcgttcat tgactcgaga acaattggcg aatgaagaat ttatgagcgc tgaaattagc
    58621 agattgaaag aagcattaga ggagaagtat ggttttaatc ttaccacgat caaggttatt
    58681 cttgatttat tagaacaaat tcaacgggca ggggctcagg ccgctgaggt agctggtatg
    58741 gtcaatacct tggttactta tgacttccct aaaataaaag aaaatgcgta cagggattta
    58801 cttactaaac tatctcaaga agaagactac ctgaatttaa aaccaggtac attaattaat
    58861 ccagctatgg ctgcagtcaa ccaattattc ttatccgcag ccctggaatt agatatgcct
    58921 tttagtaaaa agttatctat tctggacgat accacttata tcaatattct tgtcagacaa
    58981 actgaaagcg atctgcaaac attaaggaaa aagctatccg aagatccctc gaatcatgaa
    59041 atagcatttg aaattgagat taagaacgat aaattagctt tcctcagaca acagatggtg
    59101 ttgcttgaag ataaaaaccc caatcaaacc agaaatgtct tattggacat gcaattcgag
    59161 gtgtcattac gtaaccatct tgtttataca acacttaaag cacccattgc ccaggaatat
    59221 gaacaaattg ccagagaata ttacaaaaaa aacaaaaatc gctttctata tgccgatgac
    59281 tgttcaaaag agttgctgca aggattgaaa gaatttgaaa aaaaacattt gcctgatcat
    59341 ttgatagttt ttgaagccta cgacaggctt cgcctattca gtgcaaaact tccccctaaa
    59401 aatcaggacg tcaaagagta tattgaaaac atcaatagag aattacaaaa tagagatatc
    59461 cctatagaac aacgtgctat aaaagttaaa tcattaccta atgactctgt ctttattgaa
    59521 aaattaagtt cagctgataa aggcactcat ttcctaagaa aattcatgca gttcattaca
    59581 atcattacag caagcatcac cgaagcaata aaaaccgggg gcagtttgat ttatatttat
    59641 cgccaaaagc aaatggaaca atccataaag aatattgaaa aatcgataag ccttaaagaa
    59701 gaacttcata ccataaaaaa tacggatgac gcctcacccc gcaagggaga agggcaagaa
    59761 ggtgaaatcg aatttaccaa attgtaagac tcaataggct atattggtaa aaaatgattc
    59821 tacactctgg acttaccccg tgaactgtgt gtaaacagta atggaaaaat attcatgtta
    59881 agtgcctaag gttgttttaa aatcataaag taagcatcaa cataatcagg taaatctcga
    59941 gtagccctgt acaaagcgtc ctttcctgca aagcttcctg ccaaataatc aatttggtat
    60001 aaattattat taaaagcccc cccagtatca gccaggatgc ccattttagt cattgtgcgt
    60061 cctgatttat cttgatactg aatcattagt aatttcccta aaccaaactc agccaaatca
    60121 gctgcgaagg tcaccttggg gttaaccgta attttgtatt cagcgtcctt accataacct
    60181 ttaattccat cgacttcctt gaaataccaa aatcgctcct gctgataggg agttttagtt
    60241 cgatcataag caatattatt actacgatgg acattaaata ttttcttgcc cgcattcggt
    60301 ccaaaatccg cgacaacggt accttgcagt aaagccgcct ccaaatcatc tcgacttaga
    60361 taagctaatt cgctaaccgg ttgattttta agagcaccgg ataatatcgc ttgcttacca
    60421 tatctgaaac ggattagtcc tggtttggca tttgcttcat ccagagtcaa attctgctcg
    60481 tcttcgggca aggcatacaa aggatagggt ttcgcagagc ttggtttggt actggcacca
    60541 gccaaatgca caaaatattt agtcatcaat atcttatctt tgggtatatt ttgtaataat
    60601 ggtttattgg cagataattg tttggcctgg cttaagtcag gataccatcg aataaagtcg
    60661 aaatgttgtt ttacaaaatc cgggttattt aatttttttt ggttttgaca aataaattcc
    60721 aaggtttttt tcactctgtc taaagaaatc ttaatgactt ttccatcatg aatcacttgg
    60781 ggatcataat ttttgccttt atttaagtag gctaaagtat gctttgctgt actgcataat
    60841 tcagttccct taatgtcata tactccattc aaggcaggtt gggatagaaa gaattttggc
    60901 gcagcccata gtggagaaat aaacagtact gctgaaaata atgtcgctat tcgcatttac
    60961 tctgttgttt cctttttctc ttccgctttt cgagacaatc ctaatttttc ttttaaacgg
    61021 gctacggcat ccggatcata ttgctttggt gctttatgtt tgactactac ggatggtgac
    61081 ttggcggatt gcattgccac attttgtgag gcataggtag agcttctgag aggatatatt
    61141 ggcaaaagag gctcaggatg agaatcaaac gaattcattg gcttaacgct ggaaactgta
    61201 cttggttggc tatttacatg cgaatgatta gccgcttttg cattcacttg tttgcgagca
    61261 tttttaacac tcttttccac gcgtttttta attttcatgg aagcatgctc agcttcttcc
    61321 tcagtaacct cggctactgg atttccgagc aaatcgatac ggacttcacg agctttcagg
    61381 caagccagat aatcaagacg acgggtaaaa agaacaacag cttcccttaa tttactttta
    61441 gaaattccaa cttgctctgc tttttctgca tgctgcaata tatcagacat aataccaatc
    61501 tttaatgggc gaatacgcaa agaattatcg aatgcctcag ggaaattagc cgcaagccat
    61561 aataatgcgt cagatcgcgc tcgcctggat tgattttttt gtgctttatt gatgacagcg
    61621 gttcgtgggt gcagcgcctg ctttctcatt ctggtaccct tattgtgttt agtctattgg
    61681 catgaaaatt atcaaactca tttgatttgc aaaagtctgt tgaccttgaa tgtatgatag
    61741 tcatatcttg aagtcagatg gtgtaaatta tgcaattagt tactcgagga atgagcgcac
    61801 aatgtacaat gtttatcggt tgtttgcaag ccttctttat tatttccatt aagccgaata
    61861 ccttttgatt tgtatccaat tagcaaaata cattatgata tgtctataat taacttaagt
    61921 cagatagaat agagcagagg atgcttgcca gatttctaaa taaaggaacc ttatcgctca
    61981 atcatgtatt aatggtgatt acaaaatgag gataatactt tcaattcttg ctatattcat
    62041 gccttggttg gttttattga ttaaagacaa tcctggtggc gctgttgttg ccttgattat
    62101 gcaggcgaca ctgattggat ggccttttgc ttcaatctgg gcatggcggg cagtcaatcc
    62161 acttaccccg gcgtctcact ctaaacaata agtatcccaa tcctgtcttt atccaacata
    62221 taactaaatc aggacttaag aaaaatgcca agtctaggca aaaaatgttt ttaatgaggg
    62281 agtttagata ggctcaatga ccgaattaaa aattttttta tttataaaga gattggggtt
    62341 tttcggaaat catgtaaata attcggagtg ataaatgcga acacgtgtca ttgttaatgg
    62401 tgccaatgga aaaatgggag tactcgcctg tgaaacattg gaaaaccatg aacagtttga
    62461 agtagtagcc aaactaagca gacaggacaa tcttgggcaa agtatactgg acaccaaagc
    62521 acaaatagtt gttgatttaa caagagcaga ctgcgtctat gaaaactctt taaccattat
    62581 taatcacggc gctcgaccgg ttattgggac atcggggttg attgaagcac aaatcgatga
    62641 actcaccaaa ctgtgtgaaa taaaacaaat ggggggaatt attgcaccta atttttctct
    62701 cggcgccatc ttaatgatga tgctcgcagc gaaagcttct gaatattttt cagaggttga
    62761 aattattgaa ggccatcatc aacaaaaatt ggatgcgcca tcagggactg ctttaaaaac
    62821 tgcagaaatg atagccgcag cgcgcaaaaa acccaaaaac aaacttccat taaaagaatt
    62881 aacccctgga gcccgaggcg gctctcatca tgatattaat attcattccc ttagactgcc
    62941 tgggttgctt gcccggcaag aagtcctctt tggcaatata ggcgagacac tctccattac
    63001 acataacagt atagatcgcc gctgttttat gccgggaatt gtgctggcct gtcaaaaagt
    63061 attgaattta actaatctgg tttatggtct ggaacatttg ctttaaattg caaaaaccag
    63121 gttgacccgt atgactgtcg ggcatacggg tctcttttcg catttcaccc cttgatatgg
    63181 agcgtttccc aactcaattt tttaaacatt acacataaac caatccactg ttttctcttt
    63241 tggaactctc aaacaaagaa gaccattcta aaatcgcttc ttctgccagt gtttgtaatg
    63301 tgatttctga tgacaggggc tgtaataatt tcctaatacg atcatagact gaattggttg
    63361 ctgtagttct tgatgagtcg ctttctggcc gatttaaaac agctgcaaat atttttccaa
    63421 tccgaagaac attatcattg cccgagtttt catcatgcat tacttcacga atattgagta
    63481 ttccagtagg tgttgagctc acatctttat cagagaacca acgtaatttc caggaatctt
    63541 tgcttgattt ttgaaatctg cgctcctcat tgattaaagc gcttagcaca tcataaagca
    63601 ttgtacacat tttttcgcct agctgtttgg caattaacaa gcaatgcaat tcgttttctg
    63661 gtagaaaaaa ggaacgcaaa tccttgctaa tattcttcac ttcattatca gttgctaatc
    63721 ggtcatcata ggctaatgct ttctcagtac ccaagcgctc gttaatagcc tcagcaatat
    63781 cgttgggcca ataccaatca ggcgttttga ttccctctga acctggcgca ttttgccctg
    63841 caagctcatg ttgatgtctg gagatataaa gatctacaac aatattgatg aaattgactc
    63901 gttcttgttt ttctttggga attccaaatt taggccaatt tccatatttt gctttataca
    63961 aaatcattgc atcagtatgc aataactcca ctgcttttct gtctttacca ctgacacagg
    64021 agccatagct atagccaccc atgttgagaa taatcagttc ttccaaagag ctaagaaata
    64081 attccctgcc atcgtaatcc caaaatgttg cagagcctaa aggcgactct aaaacagcct
    64141 tataatcatc gattagcgcc tgcaatccag gcttggaaga cgcatatttc tgcgctgttt
    64201 ttaataaaaa ctcgctgtct gtatccgttg cctgagtata ataataacgc ttggcaatat
    64261 taaaaggatg attatgctgg aatatttcat gcctgcgttt gctccgggta accgcttctc
    64321 gagcaatttt atacaactcc agatccggag gtaattccgg taaaaagtct gtaaccaccg
    64381 ttggaatata atctactgca tggatagggc taattaatgt ttgcaataac aaaggctgct
    64441 gaggtttggc aaattccatc accttaacca ggttagagtc aacatgacgc tgctggacat
    64501 cctcaggaag ttttaacgaa tcacgactgg caacatggct tgagcgatac ctcttgtcat
    64561 aaaataaagt ttctttaccc tcaccatcga ttaaatataa actgtgcgca ccataattgg
    64621 ctggagcagg caaagttcga tgccggcttg ataaataaga tacgacatcc tcaacctttt
    64681 ctgcattttt tagagcatat tccaaaaagt attgttgttt ctcggacaaa acccaatacc
    64741 actgtggaag cttaggcact aggctcaaag tcgatgcgta ttgatcattc ctggcctgct
    64801 caaccataaa ttttttaaac tcttttaagc tggatttaat ctcatgtgga gtagcagaca
    64861 agacacgaat catggcttgc tgagcagggc ttaattcatt gaaccaggaa ggataaggcg
    64921 gtgagttggt atgtatttga tttaattccg tggtcaaatt cagtgacctt tttgcaatat
    64981 ctccccaatt gccaaaaaat gtattaaagt gctgcagcgc ttttttagga tcaacagaac
    65041 ttaaattcag attacaataa taagcttgtt gatagggcgg cagattttta aaccaggcag
    65101 gtgttttagg aaaattatat tcttttaatt gttttaattc agttaaccat tgttcataaa
    65161 cgggagacag ggattcatca tattgcaaaa catactttgt ctcactgtcc atttcaatag
    65221 gcattatggt agcgatatcg ctacgacccc gactcattaa gacatactgc tctgcttcat
    65281 taaggctggc tatcgcttct ttgactgcct tgcctttagg ccatttccac gcgatggcaa
    65341 tagcttcaac cagtttggct gtaaatcgtt cgagctcaag atgcaactct tctaatgtca
    65401 gtttttccag gtcacatgaa gccttgcatt ctgcggcata agtacttaag gttgcaaccg
    65461 tcagagaagt atgttttgga tcgagggggt aagcttttaa gataaatgaa aaggcatcat
    65521 gaaggctgtc tatccggcga gcgatgcttt tacgataatc tggctgctct ttgaaatact
    65581 ctactttgta accaggtaga agtcgctcta gtgtatcaaa tacctgagga gataatattg
    65641 tattttccaa aacacgtaaa attatacctt ttcccatagc ttaattccct tactgcctta
    65701 a
//
LOCUS       DBNCWK010000005        55044 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.005, whole genome shotgun sequence.
ACCESSION   DBNCWK010000005 DBNCWK010000000
VERSION     DBNCWK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 55044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55044
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.005"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            374..901
                     /gene="aroK"
                     /locus_tag="AC06IO_000265"
     CDS             374..901
                     /gene="aroK"
                     /locus_tag="AC06IO_000265"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HIO9873483.1"
                     /translation="MSIVKVRNIFLIGPMGAGKSTIGRALAKELKLEFYDSDEVIEER
                     AGADISWIFDIEGEEGFRRREQKVIDELTQKTNIVLATGGGVVITPENRNALAGRGTV
                     IYLKTSLQQQFERTKRDTKRPLLQTEDLEGRLESLRDEREPFYDELADVSFETDKLTV
                     KAVANNIIKYLYGEV"
     gene            888..1997
                     /gene="aroB"
                     /locus_tag="AC06IO_000266"
     CDS             888..1997
                     /gene="aroB"
                     /locus_tag="AC06IO_000266"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIO9873484.1"
                     /translation="MAKFELYAEVDVSISGHQYPIIICRNGLIDPDLINRFITSKQVL
                     IVTNRTVAPLYLGHLQSVLPSKQCDVVILEDGEEHKNQRSLFTIYDSLIQNKHHRDTS
                     IIALGGGVIGDMAGFAASTYQRGVRFIQLPTTLLAQVDASVGGKTAINHPAGKNMIGS
                     FYQPQAVIIDLNTLKTLPEREFRAGIAEMIKYALLVGGSFFERIQDVLQQGLTVHSPE
                     LPLLIAECCQVKAKIVEQDERESGLRALLNLGHTFAHALETYTDYKKWLHGEAVAIGL
                     YCAAVLSEKKGLLDNLIVDQVEKMLIHAGLPHKIPNSIDLIQLRELMSLDKKIKNNCL
                     RFIMIKKPGACYIDDSVTEDCLHNTLINVVEGEQK"
     gene            1994..3445
                     /locus_tag="AC06IO_000267"
     CDS             1994..3445
                     /locus_tag="AC06IO_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIO9873485.1"
                     /translation="MIDGKEPLDEGVANQPRTLFKPGAWLAKVDFINHLILFNNVLIT
                     ILSEKEGGKTCFNSLLKSHLNQQIKSVSFKAKPPFDSQDIINEFARQLHLNNKDNNIG
                     SLAAQINERKTHVLLIIDDAHHLPESFIKEVMIIIKNQEDFGFFHLCLISDYSIVATL
                     NNLAVEPFNNLIHTIELGALSETETRTYVLQRAMASHLINRPLTDTQFKQFYHLTKGN
                     LSKINSNLELFLSKCANEKQNNKKAILKRISLVASFAIVAGLSYFYFDNVYKAPDNTL
                     KLPDLTSVTTLPPINDLIKTPTQQAVPKQESVSYIASWEDSSTRQLVHYGLPKKQILD
                     DFMDEEDSLNTVAVVDKVVVIPKLKAKNISEQVGQAVVSQSKLPPIPHSSQTKLASEQ
                     PGIKKINSKLFTIQLAASHKVEDINRFKNQNKWLAQAKIRHFTNAKGSWYILTIGEYE
                     SRNQALLNIKKLPGELAKLSPWIRSVEGLSNIG"
     gene            3554..3985
                     /locus_tag="AC06IO_000268"
     CDS             3554..3985
                     /locus_tag="AC06IO_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIO9873486.1"
                     /translation="MYKRVLFATDFDEVGVRAAEKAKKIADENGADLILVHVVEPIPA
                     YAYPGFAGFAEVEMSIREQAEKELNELAEKLGVDEKHRFIEYGSTKNEILRVAKEHKI
                     DLIVTGSHGKHGLSLLLGSTANAILHGAECDVLIVRPKISH"
     gene            4109..5095
                     /gene="ribF"
                     /locus_tag="AC06IO_000269"
     CDS             4109..5095
                     /gene="ribF"
                     /locus_tag="AC06IO_000269"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIO9873487.1"
                     /translation="MKLLRSSTHFAIFDKGAVATIGNFDGVHLGHQHLIKALRAKADE
                     MNLPLVILLFEPQPKEYFHREKAPARLSTLREKIDVLNLCQVDYIYCIKFDARLAQTP
                     ALYFAQFYLFEALKIRYLLVGQDFRFGKNREGDVNLLKTLGANYSCEVTVQSDFLIEN
                     EKISSTRIREALQQGNLRFAAKLLGRPYSMCGRVIKGDGRGRQWGIPTANLGIHRLSI
                     PLQGVYAVQVHINSNVFYGVANIGKRPTVAGTKNILEVHLFDFHGSLYGQLVQVIFLH
                     KLRDEVKFSSVEALITQINSDIILAKTYLKQNWLDSAIDSSNDVVNGISNTV"
     gene            5208..8003
                     /gene="ileS"
                     /locus_tag="AC06IO_000270"
     CDS             5208..8003
                     /gene="ileS"
                     /locus_tag="AC06IO_000270"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIO9873488.1"
                     /translation="MAEYKDTLNLPNTSFPMKASLSVREPEMLADWQAKGIYKKIRKA
                     RDGSKRFILHDGPPYANGHLHCGHALNKILKDIIIKSKTFSGFDAPFVPGWDCHGLPI
                     ELNVEKKVGKAGSKISPREFRAKCREYAASQIDIQRDEFQRLGVLGDWYNPYVTMDYH
                     YEANIVRALGLMIKNGHLQQGFKPVHWCIDCGSALAEAEVDYEDKTSPSIDVAFSAVN
                     PSEFLNCFETQPAVKPLILPIWTTTPWTLPANEAVCLHPEIDYALIDAGNSYYIVATD
                     LVESVMARYGISHYKTSGSAKGRVFEHFKLQHPFYKRQVPVVLAEHVTTESGTGCVHT
                     APAHGPDDYLVGQFYQLPLINPVMANGCFAENVELFAGISVLKANETILAVLSERNVL
                     LASESIRHSYPHCWRHKSPMIFLATPQWFISMDKSNLRQAIINEIDKVNWVPDWGKAR
                     ISNMVENRPDWCISRQRSWGTPMPLFVHKTTRELHPDTLELIERVAMMIEKSGIDAWF
                     DLDSSELLGDDAKHYDKITDTMDVWLDSGISHYSVLKHNNDLDFPADVYFEGSDQHRG
                     WFNSSLTTAVAMYGVAPYKTVLTHGYTVDAEGKKLSKSKGNYVALDKLVNQHGADILR
                     LWVASTDYRHEVSISEEIIKRNADAYRRIRNTARFLLANLFDFNPASDCIEAKELLEL
                     DRWALKRCQLLQEEIITAYENYHFHLIYQKIHNFCAVDMGSFYLDLIKDRQYTTAKDS
                     IARRSCQTAMYHMVKAFTIWLAPILSFTAEEIWQTIPGNNSESIFIEHWYNAWPTIDA
                     VNMEDWEQLHIVRDEVNKALEETRQRGEIGSALAAEVTVYADAKVLPKLTRLGEELRF
                     LFITSEAKACPISQSPKGLAVTDCGVSIQVTASVHEKCARCWHRREDVGQNQEHPELC
                     LRCVGNISGYHEERLYI"
     gene            8000..8464
                     /gene="lspA"
                     /locus_tag="AC06IO_000271"
     CDS             8000..8464
                     /gene="lspA"
                     /locus_tag="AC06IO_000271"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIO9873489.1"
                     /translation="MKKWPWLVLSFLVIICDQLSKYWVGVLLTPYKPMPVMPMLNFTL
                     AFNTGAAFSFLSGAGDWHRWFFAGFSFLMSIILLIWLVRTPEQARFQSVGISLILGGA
                     IGNLIDRGLHGYVIDFIDVYYKHHHFATFNLADSAICIGAAILVLDLLIRRE"
     gene            complement(8761..9324)
                     /locus_tag="AC06IO_000272"
     CDS             complement(8761..9324)
                     /locus_tag="AC06IO_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HIO9873490.1"
                     /translation="MIIEKILGNQSVIISLDIDNFLFDRLDQIANSDLDLVEINSSDT
                     NLLVKIKTQYPSLKIGAGGIIDTQQLEQCYQAGVHFASSPGFLASIAQTASVYSMCYF
                     PSVATISEAMAAANIGFKQVRPYPANLTFCNALSKYLPGLNLFPAEIDWNEAELFLNL
                     PAVAAVIIHNPDHKQLNGLARLAQSII"
     gene            complement(9500..11689)
                     /locus_tag="AC06IO_000273"
     CDS             complement(9500..11689)
                     /locus_tag="AC06IO_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LidA"
                     /protein_id="HIO9873491.1"
                     /translation="MAKDNKSHQVKTSEGSLESVKTKEKEPVVEKMRVEDSKKEDKLS
                     MPTTKKESQPNEPAKPFKTSFEKWIESSLLDPQAKEDKGSTINLGREGLKNASQVKKF
                     LLSPAGKDVIAELGAQMALQRNINLQNQQDRMEHELFKRRLMAALFLWYLSKKSHAAE
                     KVKEIIREYNEKAIKNAEKASKPSQQSTPSTSQADKEIQKMLDEYEQAIKRAQENIKK
                     GEELEKKLDKLERQGKDLEDKYKTYEENLEGFEKLLTDSEELSLSEINEKMKAFSKDS
                     EKLTQLMEKHKGDEKTVQSLQREHHDIKAKLANLQVLHDAHTGKKSYVNEKGNPVSSL
                     KDAHLAINKDQEVVEHKGQFYLLQKGQWDAIKNDPAALEKAQKDYSQSKHDLATIKME
                     ALIHKLSLEMEKQLETINDLIMSTDPKENEEATKLLHKHNGLNLKLANLQDMLAVHRK
                     EKSFFNEKGEKVTSLNDAHYVIGKDQQLFNLGGKFYPIHKEQKILEKDGKFYLLKQGE
                     DWESIKDSPEKQKKAEHDFHKLQYETPMTVKKLVHHNKGLETTIHKERIEETKQQLED
                     NGKEKIEIANNISKLQSTVGTALNELNQSNINQESVTLTPLGGGGTTLSPKPGPSLAA
                     VTTFFRTKIQEMKEMNSPGMTRDELRQFQSQIPEGAARNYFISALTQMPRTGPVPFQI
                     MQAMLRNLERFGVDTTKPGVTSIRSKTDEVVEQRFNPTPSLSPFKTS"
     gene            complement(11931..15056)
                     /locus_tag="AC06IO_000274"
     CDS             complement(11931..15056)
                     /locus_tag="AC06IO_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873492.1"
                     /translation="MPDFETTDGQTQVIIPLRVTPHTHKAMPTITGSIFSHEAGTVFY
                     GNYNMEYLLPGGDRLRLIANHNKKTVQLMLFTHNPELLKILPGDRPSSLVADVINHVN
                     RYKVQAESYKKPLSHEQEEKINLANACIRSLNHLLKKERQLKPHDHAGQEELRRKIIS
                     IIEHCQNDNRMIANNPVVSEGSFGYMLYDAHKTAQHYRFNRVFSVSRQDQMDFTKVTK
                     TQLGKPITPCFVWDSEIHIGHSSRELDDALRVICQQYQLCPAPALNNIPANRFAKLER
                     FISKLWHDGHDWINYLAVTTKPSHKTEIQKRSDGVSITKITPYYKLKGLPQIGYNSLA
                     AMVNHLVENSAQQTEVNSLKEAKRLLSHLPNGNWVIVPQKNTVLLRLENKLIHLKYFI
                     HDQLFYPLPDGEDLYILSQISKRHLYLPERISLRFKAFISRIPDFFKGFYKSMSQFII
                     HDLHEEFFNHVHATHAPKQHLSERDQRPKSKKIKRTSLHEALQNQGLLANGQTLEEFI
                     SDQINNSPYVIARANHPPTVHTYENPLHRALGVVRHLAGFFIDTSERNPMIGTLAMAA
                     YVYGAGAVLAPNVLTDILKKLHLSGLIYGIEPVQKLARLMNHGKISEAISASATLWQG
                     MVVSGNLDKFFVDAVSVLKEDPAEIAIIVSLALSLGYGLTKAIPSLQHEMGDFPYANY
                     AALGGKGGAAIYDTIMHPGDDWFLGTCKWFCKNIINIGKLLVAPFFEGFYYGFFDGFI
                     NGWKKSGKLIKQLGKQTLAASFDLILAILTIPLLEASSLLIHVPFRGITNFFRKLLSV
                     LGNFSVIGKLLIDIAERPSTNNFISEFTLSPLYGFTSPFGHFSDNPVTNIGINVIRLL
                     FLPLLQLIKNILILPLIDILSLTIRVNLSIINPISRLLAYSTGTILYHFGQIWDHSAG
                     VLFSTSAYGVTILCNHIDSKAGELKQYLLSLIEINRGKVYQWAFHDEDILTHKIVQDN
                     QYYFNDPRRCELIPHSDSHCLLRNLLLDEKSSTNKVKDNQKSQQLHFPGLFHGATQTS
                     DTMPQEHTLTI"
     gene            15270..16205
                     /gene="era"
                     /locus_tag="AC06IO_000275"
     CDS             15270..16205
                     /gene="era"
                     /locus_tag="AC06IO_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIO9873493.1"
                     /translation="MTWLLECFKNKQESRLMSSYCGYIALVGRPNVGKSTLLNRILQQ
                     KLSITSRKPQTTRHSILGIRTEDEFQFVYVDTPGIHQGNAKAINRMMNKTAISVLRDV
                     DVIAFLVDGTHWKDEDEYVLNLIKQAKVPCILVVNKVDKITDKTQLLPWIEQISQRYQ
                     FAAIIPLSAKTGLQVDELEGKLKTYLPEGPHLFPDDQFTDRSIKFLCAELLREKIFRF
                     CGQELPYSVTVDIESFKDEGNLIRIHALILVDKDNHKRMIIGDKGQKLKEMATNARLD
                     MEKMLDKKVFLQCWCKVKSGWSDDERILKQLGYDQ"
     gene            16195..16884
                     /gene="recO"
                     /locus_tag="AC06IO_000276"
     CDS             16195..16884
                     /gene="recO"
                     /locus_tag="AC06IO_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIO9873494.1"
                     /translation="MTSKSLNAWVIHKQWSGDTSARLKLFTKELGLINCLCKGGRTPK
                     KQSLLQAFIPLWVSIEERYDQYYTRNIESTSSRLDLEGHSLFSGLYINELLYYTLSPD
                     FPDPDLFDAYLFTLNGIALAREREAIEALLRRFEWALLKACGYTFSFLHEARTGELIV
                     PDSHYQFVAGEGFILGGDKKIPGEHLLAIAADNLSESAYLKSAKFIMRQAIDHLLGGR
                     EIKARSLYGPA"
     gene            complement(16940..18115)
                     /gene="ravJ"
                     /locus_tag="AC06IO_000277"
     CDS             complement(16940..18115)
                     /gene="ravJ"
                     /locus_tag="AC06IO_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavJ"
                     /protein_id="HIO9873495.1"
                     /translation="MAIAPQQIQERLKQEQYQKFVVADIGNFPHCLAQTPEGIASGQR
                     YQKYSTNSLSRTPPFSQWGAPQLLTPKSAQEYIKFAQQRNKKSSFKIDGEAVRVSECS
                     NFAYHAAGVLLDDPQIRTQYDVAVIGSMHSNGRYLHNITLLVPKGSRLPQPPQQLTAE
                     VFPTGTLIVDPWAVGMGHPPEQALAIPKEQFAYNRSLFPATVNYQSALDESLTSTRTG
                     QLTPYTGTPSQTGRQEVRPESQFPDTRRSVSATFPGNFAAYHELWRNAFQEIMNDPRH
                     QLHRNDVEYKKIHAIRTVLDDYTKGGDTWWAKFRRIFTFHWNRHHVKVVDDIVKEIDA
                     GNYTTSRALVDRLDNLAISLGSKLNPKGTLKEQIGFIKIQEQNPVEEIENLSRPHLT"
     gene            complement(18277..19167)
                     /gene="legL1"
                     /locus_tag="AC06IO_000278"
     CDS             complement(18277..19167)
                     /gene="legL1"
                     /locus_tag="AC06IO_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL1"
                     /protein_id="HIO9873496.1"
                     /translation="MVLTLSDLEKGYDKNLNQLSLSFLNLRDNDIPLLCEFLQNHPAI
                     TSLDLSHNDITANGMKLFVNKTSVSSLNISHNNIGPEGAQWLSEDNHITTLDVSFNEI
                     GDEGIKALAANAKLITLYALYNKITKVGAGYLAQSNLKKIDLCFNSLEDEGVIALASN
                     INIKELIASACDVSDIGAIELAKNNQLTLLILGKNAITDKSTLHFANNTSLSTLHLGS
                     NQITAAGKKILETNTRITDLDLIGNPIEVHETDELNYSKKSANSHSVFKFLQKFTFLS
                     CMSPCQETAESDKGLNKKPN"
     gene            19456..20202
                     /gene="pdxJ"
                     /locus_tag="AC06IO_000279"
     CDS             19456..20202
                     /gene="pdxJ"
                     /locus_tag="AC06IO_000279"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIO9873497.1"
                     /translation="MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGIT
                     LHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAMLDFAEEISPEHACLVPEKREEL
                     TTEGGLDILTHRNVVEKAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
                     ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHA
                     IIARALFCGLKEAVRHMRQLMQEARLYVND"
     gene            20189..22006
                     /locus_tag="AC06IO_000280"
     CDS             20189..22006
                     /locus_tag="AC06IO_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIO9873498.1"
                     /translation="MSTTDVIVVRITGDSGDGVQLVGEQLTISAALTGRDVRTMPDFP
                     AEIRAPAGTVAGVSGFQLAMAERAIFTAGESLDVLVALNPAALKNSLQHLNQGGLLII
                     NEDSFQERDWQKAGIDKSFLDNCREHYHIVAFPLITQTIQAVESINLTKPQAVKTKNF
                     YILGLVLWLFDLPTETCQAFIAKKFKTNQAIAKANELALLAGYNYAMTLELSRRDYML
                     GEVNRHSGEYRQITGVEAVGLALATLATQTQVPLLVSGYPITPASAILHECVRLSDYG
                     VQLIQAEDEIAAICSCIGAAYGGRLALTCTSGPGLDLKCESLGLAVVTELPLVLVDVQ
                     RAGASTGLPTKTSQSDLKQALYGRHGEAPLPVIAAQSPADCFNTIIEAFRIAIKYMTP
                     VIVLLDAYLANAAEPWEIPALDSLNIPKPEFNRFTKPYHRDEFLSRSWNVPGTSGFIH
                     QIGGLEKQGDEGRVSYDAENHQKMVITRAEKIKGIARDYPALVLEGDMTAPTLVIGWG
                     STFGSLKSAVHQCLEQGLPVAYLHLRHLNPLPDDLGSLLKKYDKVLVAELNSGQLCQL
                     IRAVYLVDAQSISQCNGQPFSVNHLVSAIKAEANYEQQL"
     gene            21990..22958
                     /locus_tag="AC06IO_000281"
     CDS             21990..22958
                     /locus_tag="AC06IO_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIO9873499.1"
                     /translation="MNSNYKREDFVNETEVRWCPGCGDYAILAALQRVLPELGLPPEQ
                     HVFVSGIGCAGRLPYYMNTYGFHTIHGRATAVATGLKAMREDLCVWVITGDGDALSIG
                     GNHLLHILRRNVNVNILLFNNQVYGLTKGQFSPTSQKGQVTKTSPKGVTSEPINPIMI
                     ALASGASFVARAVDKDPVHLASVLKKAYEHKGCSFVEIYQDCNIFNHGAFDDFAVKNN
                     RAENTIILEDGQPLVFGAQKEKALQLDDEQFVQISVDEKALYKHDAKSLISAMRLARL
                     TFPDYPVPLGVYYQKDRELFTLSQEVKKRPEDLPELFKAKASWKQN"
     gene            complement(23149..23823)
                     /locus_tag="AC06IO_000282"
     CDS             complement(23149..23823)
                     /locus_tag="AC06IO_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIO9873500.1"
                     /translation="MNRNDITILSQQRESVLATNKVLRNTYLLLSLTFIFSALTAYVA
                     FISGARPMNPLLMIVGVYGLMFLTQALRNSVWGLVSVFAFTGFLGYTIGPLLNYYITG
                     FSNGPQIVATALGGTGMIFFALSGYALTTKKDFSYLGGFLFVGVMVALLAMIAGIFIQ
                     IPALQLVISAAFVLISSGLILLQTSAIIHEGETNYIMATIGLFVSIYNLFVSLLNLLS
                     AFSGRE"
     gene            24042..24848
                     /locus_tag="AC06IO_000283"
     CDS             24042..24848
                     /locus_tag="AC06IO_000283"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIO9873501.1"
                     /translation="MNRVALVQMVSSAKIADNLQLTEQYLIQAREQEASLVVLPENFA
                     FMGMNEREKLHIAEYYGQGPIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYD
                     DKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
                     ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRH
                     TYGHSMVVEPWGKVLAQKEEGQGVILADIDLERLSQLRRQFPCVEHHVLM"
     gene            24860..26302
                     /gene="tldD"
                     /locus_tag="AC06IO_000284"
     CDS             24860..26302
                     /gene="tldD"
                     /locus_tag="AC06IO_000284"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIO9873502.1"
                     /translation="MTKSLAIAKNILLTPASLDEAGIEKLFKTMFNRNVDDADLYFQS
                     CSYESWYLEDSEVKSGNFSLDKGVGIRAVSGDKTGFAYCDDILLPSMLRAADAARSIA
                     LSGSKTVPAVKVLKASVNRYESLNPLEGMSKQEKIALLEAIDKEARSLDPRVIQVNAT
                     LTGCYEVVMVANMDGQMMADVRPLVSINVSVIVEDSHGRRESARSGGGGRVAYSYFTE
                     KENALSYAREAVREALTNLKAEPAPAGTMPVVLGPGWPGVLLHEAVGHGLEGDFNRKG
                     LSAFSGRLGQQVAAAGVTVVDDGTLENRRGSLTIDDEGTPSQCTTLIDNGILVNYMQD
                     KLNAKLMGMQSTGNCRRESYAHVPMPRMTNTYMLSGQYDPQEIISTVKKGLYAVNFGG
                     GQVDITSGQFVFSASEAYLIENGKITKPVKGATLIGNGPDVMKKISMIGNDLALDRGI
                     GVCGKEGQSVPVGVGQPTLKIDALTVGGTK"
     gene            26423..27301
                     /gene="rssA"
                     /locus_tag="AC06IO_000285"
     CDS             26423..27301
                     /gene="rssA"
                     /locus_tag="AC06IO_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HIO9873503.1"
                     /translation="MTNQYPKIGLALGSGSARGWAHIGVIQSLKRLGVRIDCVAGCSI
                     GALVGAIYACGTLDLFEQWVLKLNKREMAKLIDVNFLGGGVLSGSKLMTFFKDFGLEK
                     NIEELSLPFSSVATDLSTGREIWLSKGSLELAIRSSMSLPGLFTPQRYKDCWLVDGGL
                     VNPVPITLCRAMGADVVIAVNLNHDLLRVPPSTTEHPGFFSVLSGQFDKMIKRIMGIS
                     DGPGYYDVLTRSIKIMQDRITRARAAGDPADLTLVPQLGNIMLYDFHKAKEAIHEGEN
                     CVLREKNNILQLVGLE"
     gene            27328..28995
                     /locus_tag="AC06IO_000286"
     CDS             27328..28995
                     /locus_tag="AC06IO_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIO9873504.1"
                     /translation="MQKESNSNSLFDLLLKNEKNSADRVYLRQPKNGVWHEYTWSSVM
                     LQARKVAGFLHHLGLKKGDHVSIISKNCAEWFIADFGIHIAGMVNVPLFANQNEESAH
                     YVLEHGDVKLVFVGKLDDHQRVRQFIPKSYPTIGFDYHKDLQVDYEWSDVLKSDPMIN
                     LVEPEPDALYTIIYSSGTSGAPKGAMYTNRSIANYLTVFPKDLLRIRELEHYKLISYL
                     PLAHVYERSAIQLGSVAINASVSFVESLEKFAENLREVSPTFFTAVPRIWGVFKQKIE
                     QKLPPAKLNFLLKIPLISSLIKRKIIHSLGLSECTNCFSGASHLPISIIDFFEKLGIY
                     IQEGYGQSENLAYATLSMLNERKRGYVGTPRLEVEIKVGEENELLMKCPCLMSGYYKN
                     EQATKNAFTEEGWLRTGDVVDVDNEDRVKILGRLSENFKNQTGEFIAPSPIEKLFSVN
                     PMLEQLCLVGRELPSNVLLVTLNEGVRLGMPKEEITHALQETLKQVNSKLVKYEKVSH
                     ILVLKDAWTTENDKLTPTLKVKRRVVEKDYAELIQKALQKSQSIVWE"
     gene            complement(28974..32435)
                     /gene="mfd"
                     /locus_tag="AC06IO_000287"
     CDS             complement(28974..32435)
                     /gene="mfd"
                     /locus_tag="AC06IO_000287"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIO9873505.1"
                     /translation="MNTKTLLYQPTKNKQIWGQLYGSSLALALAEYCQQTPGIKLLIT
                     QDNLCASQLQDELQFFLNPNQNTQELLFFPDWETLPYDQFSPHQDIISERLYTLSRVQ
                     QITNGIIISSASTLMHRLSPPEFLNQYALMLKEGQKLDLNLFRNQLQQAGYHCVNKVL
                     EHGEYALRGSIIDVYPMGSGLPFRIELFDDEIESLREFDPETQRTIEKINQIHLLPAR
                     EFPLNEQSQLHFRRAFRESFPGNPSHCPIYEAISNNQFPSGIEYYLPLFFDKTVTFFD
                     YLPANAKICLIEEIQENAERFWRELNERYEQRRHDVSRPILPPDNCFINPTELLTKAN
                     HYEQVRLYHKPSEKKGALNFNIAKAPQLPIDRKNIEPLCQLSEYCSDPTRRYLIVVES
                     AGRREVLLDLLKSSHIIPKTQNSWLDFIYDESPINITTGPLIYGCELTDKQIVIIVES
                     QLFGEQSTPQRRSTQKSVDPDLIIRDMAELRLGAPVVHLQFGVGRYQGLQYIDSNGTP
                     SEFLVLAYAGDDKIYVPVTSLHMISRYTGVDSEHAPLHKLGSDQWQKEKKKAAEKIHD
                     VAIELLDLYAKREAQPGHQYDFNQSEYIKFASGFPFTETPDQLQAIEQIIKDMQSPRP
                     MDRLICGDVGFGKTEVAMRAAFVAVQNGKQVCILAPTTLLAGQHFESFRDRFAEFPVN
                     IELLSRFRSNKESEAVLAALQSGKVDIVIGTHKLFQNNISFKNLGLLIIDEEHRFGVK
                     QKEHIKSLRTHVDILSMTATPIPRTLNMAMAGIRDISLIATPPAKRLAIKTFWQEKND
                     LTIREAILREILRGGQVFYLHNNVQTIERVCQDLEALVPEAKIQSAHGQMRERQLERI
                     MSDFYHHRFNVLVCTTIIETGIDIPTANTIIIDRADKFGLAQLHQLRGRVGRSHHQAY
                     AYLLTPNEKLLTPDAVKRLEAIVSLEDLGAGFTLATHDLEIRGAGELLGEEQSGNMHA
                     IGFNLFMEMLDRAVNDLKAGKTPELSAPMHQGPEIDLRISAIIPEDYIGDIHNRLIMY
                     KRIANAKTTQQLRELQIELIDRFGLLPQPVKHLFLITELKLKAAQLGIQKISASAQQG
                     KLDFSEKPSIDPGTLISLIQVHAKRYQMEGPQRLRFTLDSTSAEERIFEISSLLNKLS
                     PHSQTID"
     gene            complement(32512..33774)
                     /locus_tag="AC06IO_000288"
     CDS             complement(32512..33774)
                     /locus_tag="AC06IO_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIO9873506.1"
                     /translation="MALFSSVTLFEGIALAIALQFLLLFWVLIKQAQQKKLTEQLGER
                     IQQTLISQLHQLHIDLSQSYQTSQQFINEKIAQGQLATQQLISDAIQRQMADVREQMT
                     HSFKHHASSLTSHLQLLTEEIRNHLNTLTQQVNHKLTEGFEKTSSTFIDVVKRLTIID
                     EAQKKITELSSHVVSLQDVLVDKKARGAFGEVQLSTLISNMLPSASYQMQYTLSNQKR
                     ADCVLFLPEPTGNIVIDAKFPLETYQRLINSDALSTDRKSLQQQFRQDIQKHIKDIAE
                     KYIVPNETTDGAMMFIPAESIFAEIHANYPDLIAMSQKLKVWLVSPSTLMAVLTTARA
                     VLKDDATRKQVHIIQKHLQALANDFQRFEKRMDKLSKHIDLAHQDVNEVNTSAKKITS
                     RFQKIESVDIELDEHEVTQLELVESESI"
     gene            complement(33778..34956)
                     /locus_tag="AC06IO_000289"
     CDS             complement(33778..34956)
                     /locus_tag="AC06IO_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4785 domain-containing protein"
                     /protein_id="HIO9873507.1"
                     /translation="MKTAHLIWISAFCIAQAHAFTLPRQPTKSYECENCSQLSRENLH
                     DKWEITNEPLNNNISNVRRSYGYKERISLEQLQRGVIISTLAPGAVVRITPLQNKSIP
                     ELLIKTPKNQLLPLKEASSLYNQDDEVGNNPLAITKHQAMLQIKPELGYGKFILKSKD
                     ITNKYADAYMISVLDKFSITYLEVETDSLHYQYGDKLKATISLHNDITEYDVNDVDAR
                     LVGPKGQVISLNLTKLKSNVFEGTATLDSELNDRGENWYLETDVQTEYGQEIIRRSGH
                     TAFSYSIPSASLMNVKKLSSKPLTFVVTVDVATASRYALQSVLFQKNGKGEARPIQTS
                     QRAQWLEPGKHVLQFTFDNHNQLSDDNLYLGYLRLIDYGQLKTVYQYNQPVKLSQLVD
                     "
     gene            complement(34953..35852)
                     /locus_tag="AC06IO_000290"
     CDS             complement(34953..35852)
                     /locus_tag="AC06IO_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873508.1"
                     /translation="MELLRILSTLIVLLFSTLIHSHSDTNSNQGIAFVHGTRDHREDA
                     YGGYWKIDFIQSISQTLAKPENHYVVHCDFSQYMWHEDAANCVANQLLEFINDKNVSS
                     LTVYTHSDGANIMRWILSNPTYDGRYMTLKRKISQVIAIAPSSGGTPLADEVLNGGIF
                     ETSLAWLLGYLSDAVKQQRVGDMLIYNEELLLGSKGRPSLSIPFKVIVGTDVYASPFS
                     TSSYCNGYMLNSGLKIAKLYLNQCADGFLDCESQAQAGEVWFYDIDKTDYNLPLSHNQ
                     SRHSCFGLDQILISALVNEGVVK"
     gene            36074..38095
                     /gene="ligA"
                     /locus_tag="AC06IO_000291"
     CDS             36074..38095
                     /gene="ligA"
                     /locus_tag="AC06IO_000291"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIO9873509.1"
                     /translation="MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKAL
                     QQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQPMLSLSNVFTTDELKAFIKRAIE
                     KLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
                     RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEIS
                     ANRPLAIYCYGIGAFEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGIEGCLDYYQYM
                     LAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQV
                     GRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVS
                     VILEKRPANARMIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRK
                     AMYIEGLGSVLIDQLVDEGIVHHLADLYELDLQTLASLPRMGEKSAKNLLSALEKSKK
                     TTFNRFLYALGIREIGEVGARVLAEHYCDVEGLKAATIEELMTLNDIGPVAASHVVHF
                     FAQAHNLEVIDRLLKLGIHWPKSEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLL
                     ALGAKVSGSVSSKTDYVVAGSEAGSKLIKATELGVVIIEEDEFLKWVNS"
     gene            38224..40380
                     /locus_tag="AC06IO_000292"
     CDS             38224..40380
                     /locus_tag="AC06IO_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9873510.1"
                     /translation="MLFLMPCCVNLAYGSSWVFNNPYPESEANENIYYSSFNEQPKTL
                     DPARSYSSNEYLFIAQIYEPLLEYDYYKRPYELIPLTATRLPQIRYLDAQGNPVPAGD
                     NTKPAYSVYTIQIKKGILYQPHPALAQKKDGSYRYHHLPEDYLEEHDINKLSDFKYTG
                     TRELIIDDYIYQIKRLANPAVSSPIYGLMSYYILGFKEYGDSLPKGFANKNSFIDLRK
                     YPLQGVKKLDDYTFEITLKGQYTQFLFWLAMPFFAPVPWEADLFYSQKDMDDKNLSFG
                     WYPIGTGPFMLTENNPNRSMILEKNPNFREVYFPVNGSDADRRAGYLTNQGRRLPLID
                     KAVYTLEKESIPRWNKFLQGYYDMSGISADSFDQAIHINRFGEPILSQEMKDKKIYLR
                     QTLEPSIYYTGFNMLDSVVGGNSKRARKLRQAISIAVNFDENIAIFFNGRGIPAQGPI
                     PPGIFGYKEGKEGINPYVYQWVNNARKRKSLKDAQKLMVEAGYPGGIDPKTGKHLILH
                     YDVMTTGGPDDKARLDWMRKQFANIGIDLNIRATLYNRFQEKMRIGHAQIFSWGWNAD
                     YPDPENFLFLLYGKNGKVKYGGENASNYENPEFDRLFNLMKNRPNDLQRQKLIDKILA
                     IVRYDAPWVWGMHSEDFILSQDWVSNIKSNTISLNTLKYVSVNVPERNQLRRSWNQPV
                     FWPLGVLFGLLIALVIPLLITYLKKEKQPAERVKLK"
     gene            40377..41354
                     /locus_tag="AC06IO_000293"
     CDS             40377..41354
                     /locus_tag="AC06IO_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9873511.1"
                     /translation="MIKYILRRILYAIPILFGINLITFALFFMVNSPDDMARMHLGNK
                     HVEQKAIEDWKAVHGYDLPLFYNEKQTGFNQFRQTLFYQKSLMLFTFNFGVSDAGRDI
                     SHDISYRMWPSLVIALPTLLFAVLVNIIFAMAMAFFRSTYLDLSGVVICIILMSISSL
                     FYIIGGQYLFGKILKWFPISGYDDGLDALKFTILPVLVAVLGQLGAGARWYRTLVLEE
                     INKDYVKTARAKGLSEQRILFKHVLKNAMLPILTGIVVIIPTLFMGSLVLESFFGVPG
                     LGSYIIDAIQQQDFAIVRAMVFLGSVLYIIGLVLTDFSYILVDPRVRLS"
     gene            41378..42724
                     /locus_tag="AC06IO_000294"
     CDS             41378..42724
                     /locus_tag="AC06IO_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9873512.1"
                     /translation="MTFELLWTDKCFLTLLAVVLFTVLLSLRKQHIKLSFAIILQRPL
                     AISAGIVLLLFLAIGVLDSIHIKFINDKTEKTQSLLDRVLAPIGNTYEKTYSAPLALN
                     QFMSETKLVNGVTTQVYPRLKYPPASIKNEKDKSLIIKSVIMKGITYSALVILGGCLL
                     LALVKFSLGYKNTFILNSTQISAIATVAVLTFAVVIGYGLSRSFHVLGTGQIGQDIFY
                     FSLKSVRTGLIIGFLTTLFMLPLALFLGIAAGYFGGLVDDIIQYVYTTLSSIPGVLLI
                     TASVLSLQTYIANHPEQFTTLAKSADARLLALCLILGVTSWTSLCRLLRAETLKLREI
                     DYVLAARALGSSWFTIVRKHLVPNVMHIVIITLVLDFSFLVLAEAVLTYVGVGVSPMT
                     ISWGNMINGARLELARDPVVWWPMFAAFLLMFLLVLAMNLFADAVRDAFDPHQSHL"
     gene            42799..46245
                     /gene="dnaE"
                     /locus_tag="AC06IO_000295"
     CDS             42799..46245
                     /gene="dnaE"
                     /locus_tag="AC06IO_000295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIO9873513.1"
                     /translation="MRQRFVHLRVHTEFSLVDGLVQIKPLMKALPARGMSTVAVTDFC
                     NLFAAVKVFKSATEAGIKPIIGSDLPCHDLETPDNIFSLVLLCQDTAGYKNLTCLVSK
                     AYQEGQYHGQPRVQLQWIPEFSQGLIALSGGKFGDIGQALLANDDTLARKRALHWMNL
                     FPNRFYLEIQRTGRADELHYNEKLIALADELQLPLVATNDVRFIDEDDFDAHEARVCI
                     HEGYTLADPRRVQQYSSQQYLRSADEMELLFSDLPSALQNTVEISKRCNVKLELGHNY
                     LPNFPIPDGTTVEAYLSHLSEIGLEERLKQLFRDKSEGELLAARGEYDKRLKIELDVI
                     NNMGFAGYFLIVADFIQWAKQNGVPVGPGRGSGAGSLVAYALKITDLDPLEYELLFER
                     FLNPERVSMPDFDIDFCMEGRDRVIEYVAEKYGRQSVSQIITFGTMAAKAVVRDVGRV
                     LGHPYGFVDKLAKLIPFELGITLSKAMEQEEELRRRYEEEEEVKELFDLALKLEGITR
                     NAGKHAGGVVIAPSQLTDFTAIYCEEGSTQIVSQFDKDDVEAVGLVKFDFLGLRTLTI
                     IDWALATVNKQCENAGLSPIDISQIPTSDDSTFELLKACKTTAVFQLESRGMKELISR
                     LQPDCFEDIIALVALFRPGPLQSGMVDDFIDRKHGRARVDYPHPDLEPILKPTYGVIL
                     YQEQVMQIAQVLANYTLGAADLLRRAMGKKKPEEMAKQREIFTQGAVARGVDEQVATH
                     IFDLMEKFAGYGFNKSHSAAYALVAYQTAWLKAHYPAAFMAAVMSSDMDNTDKVVTFI
                     DECAHMKLQVLPPSINNSMYHFTVSDEKTIIYGLGAIKGVGESAIECIIQERNQNGPF
                     LDLFGFCQRLDLRKVNRRVIEALIKSGAFDVWKVERAVLTASLEKALKVAEKEHQNQS
                     SGQFDLFSLLEDKADEQNYVESKSWSEAQRLEGEREVLGFYLTGHPADQYRRELGDFI
                     VPVGQLNPSLHKKAIICAQVVAIRKIITKRGKKLVIIGMDDATARIDAVVFGEIFEQY
                     SDPLGTGNMLVIEGEVAIDDYNGGVKMTANQLLSIASARTKFARCLELRLNQNNAAVL
                     PSIQSLLKTYSGRCAVQFVYANGVAKANLNLGQQWCVTPSDELIGLLSNLLGEEQVIM
                     RY"
     gene            complement(46331..47572)
                     /locus_tag="AC06IO_000296"
     CDS             complement(46331..47572)
                     /locus_tag="AC06IO_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0963 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873514.1"
                     /translation="MRYILPAGALTSLNFLRDDYQSERKVDLDQPPSLIGVPTLFGGT
                     DVIARDKQTKFIKKMIVVLRANLAKEEEITTPEQWEANLTASRVVIAACLYVQSQISS
                     PKNNSALYRLINKDLGITDTNYLDDEDKEICFLAANRVVNSSLSAFSDANAALRKANM
                     KPFTEQEWSDFSKFVSSVSVKKVSTNPYSNYPVTSITQPLFGAAFSYTGATIGYLGGD
                     VISQSSKAMSTKLQITTMVGSSLLMLGTTGPMGVALFSQVIASKLVTAFFSISLAHVL
                     GTTMGILGQGVGMGIGLPLDYAYRLIWKASATIMNYYSNYPNTSQMTGIRIADGKSIM
                     NGIVIEYTPSSDLKTEEGQQTVEITEEGKLLINGKEVTIPDGGVQLPLELINELKAQL
                     KLHEEKVSEEDSSSSTLLATV"
     gene            47902..48786
                     /locus_tag="AC06IO_000297"
     CDS             47902..48786
                     /locus_tag="AC06IO_000297"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HIO9873515.1"
                     /translation="MMELANKIIRVSYMDKLYQYLLLLRLSHWSKAIFVMLGFIYTPV
                     PGYLFPALMASLSFCLIASAVYIYNDIQDRTEDSLHPFKCNRPIASEQVTLFDAIIVM
                     LLLMVAGIALGWLISKKLVVILIIYLIINLAYNHVLKLIPVFDVACIALGFMLRVLAG
                     TIGIDLAISAWLTVAATLLSLFIALNKRQLEMQLGLKNSTRKVLRRYNPAFLQRLIVI
                     TGIACFITYLFYTVYARAESFFFILTLPFAAIALWRFAWLATQDVENDDPVNIFLSDR
                     LSRINLWCFAILTFMALN"
     gene            48747..50294
                     /locus_tag="AC06IO_000298"
     CDS             48747..50294
                     /locus_tag="AC06IO_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HIO9873516.1"
                     /translation="MVLRYPDLYGIELMSAKWRWYDSLFIVLFAYLLVLQIQAIWPFT
                     IDDMYISLRYAKNWAAGNGLLWNLNAEPVEGYSNFSFVALGAISIILHINPVLTLKIA
                     GFAGLLATCLFIYLVSRFWFTKRESFIPCIWLLLYKGQVIWSVSGLETTVYQALISGS
                     VYFAFRGLGYHFYPAPRGEFKTSGFVLSGLYLSFAGMTRPETPALMLLFFLLISWDWN
                     KLSGKKPWRGAVSFAVTIGLVFLPYFLWRWSYYGLLFPNSVYCKGFNGISYSLDLNYL
                     KLIWPFAILALPACFYAQDRRHYFLWSPGIIYLLMLVNADPVVAFDNRLFLPAFALML
                     PLTLNGISYLLLSYFKKQDNYFRAALYVSSFFLALLFIPKMSLDAYRYFSQNPVHGEQ
                     LRKSVVQWLNTHAKNQDRVVLADSGLIPFLSGLTFIDSYCLNNKEMAQFPAVKRYELF
                     CQQILEEKPQIIILTALIDKGKIIYTPGDSCLKPFLDVHKEYKLGGVFSFGSAESQYR
                     YELFANF"
     gene            50361..52226
                     /locus_tag="AC06IO_000299"
     CDS             50361..52226
                     /locus_tag="AC06IO_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIO9873517.1"
                     /translation="MQKIIILTKKIIAKLPVILFDVAAIPVAWYAAYWLRYNMQPYPG
                     SLTSTHSFIALALLSIVQISCYYYFKIYRGLWRFSSLNDVIRILKATITAMVLVIPVF
                     YLTSILQHLPRSVFPLYCIILATILCGGRLVIRLHWDKPDRGNRELETKRVLVVGAGQ
                     AGEGLVRDLKRSSYYRPIGFVDDNKSKRGLEVHGVRVLGTTNQITELVNQYDVDLIFI
                     AIPSAKSATMRRIVTLCEQSHVPFSTLPSISALAAGRVEVNALRPVNIEDLLGRDQVT
                     LEWEKIARSIAGRRILITGGGGSIGSELCRQVMALEPASIAIVENSEFNLYRIEQELL
                     KSFPGIPVELSLISVTDEIAINHLFRRFQPEIVFHAAAYKHVPMLQHQVRVAVFNNVI
                     GTQVVAKASVTFGAEKFILISTDKAVNPTNIMGTTKRVAEIYCQNLNARVKTQFITVR
                     FGNVLGSAGSVVPLFQKQLQEGGPLTVTHPDMQRYFMTIPEACQLILQAMVNGEGGEI
                     FVLDMGEPVKISYLAEQMIRLAGKEPGRDIMIEYTGLRPGEKMYEELFHETEQLTSTK
                     HEKLFKARFRELDWNDLTQTMRMLQAACIEHQDEELLVLLKSLVPEFQLEPMVEA"
     gene            complement(52372..52965)
                     /locus_tag="AC06IO_000300"
     CDS             complement(52372..52965)
                     /locus_tag="AC06IO_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0967 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873518.1"
                     /translation="MLPFKYRYMSGADIMTGSSSEQLEEEYLNARREYCMAMMAVHDK
                     ARAFLSETPIDESMKQAIIKSSGVKIRPNETQDEFDERFEDEMGLTIDDYVDFALDVL
                     EKKLEPAKQSIVAATNKELGSLTSGQSGSAWEDFQHTIHEGAEYKTIEVAKCLPESER
                     CIQAFNQAKLAFRDAATNLIHAEKLDEQEEPNHGPRF"
     gene            complement(53329..>55044)
                     /gene="sidK"
                     /locus_tag="AC06IO_000301"
     CDS             complement(53329..>55044)
                     /gene="sidK"
                     /locus_tag="AC06IO_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector v-ATPase inhibitor SidK"
                     /protein_id="HIO9873519.1"
                     /translation="YIKVGIKMGGLTSEQYHSQVVGKIGYIARCMQTIDPENNLKKIR
                     EDYQDVLIWAEKNYRFEEILEASKSGKCPNDLDALSRRSLILQELLRLVSSISPFKMK
                     LDLIESQYEKMKQHVNLWKSDYHVKLNQLNQLTDYLKNAAPTPKNHFLRAMTSALQMQ
                     IAQTGITEDNEGINQLFKLGLHLLAMANEKINEQYDLFKGYVKDQPEESPFEGILPAE
                     DQKILVKAMIDYAMPKLSSKVLQDKLSALSSSDVLTKTLLDSIDRTVEENERLNALGK
                     VKLGKFGLDIREIEEIYSQALKISPQDALQYTAQQCDAQLLSMAFPDSQNYIVESISD
                     KKAKAIAELIHSKEFIYQIIKTEVFKQVDPNEKIRLQAATELYQLLGRIMDKQIHLFA
                     KMNLEQINEYIQTKTKAILDKIPERVELLTFMGFEIPTFKGIETLMTDVSHSQDNETL
                     AIAQEFYANIKNAKKQLLGDKLIEDITPQDVEKFFNQCSQYGSEAAEKLADNRPVLTK
                     IADILTAIARWAISLIGFNTPPQFLAPTRTCVDQVSDEITKIKLKLEDTLGSLQKVQE
                     ENLSL"
BASE COUNT        15920 a         9582 c        11930 g        17612 t
ORIGIN      
        1 gtgaacatgt tataattaaa atatagcccg ctatgatgta atggcatttt gaaaaaaatt
       61 gctcatcagc gatggattgg gtaaagttga agtatcaact tcagcgatgg cagaaaagtg
      121 taataaataa ttattttata tgggtaatta atggttatcc gttgtagaat gtcagaagta
      181 ttttagatag taattggatg tattcctaat ttgtgttatt tactttaaat gcttcaaata
      241 aaaggtcttg tgttattcta tcgaggctgt tggagcagta aatatcatga aatcaactaa
      301 gacgtgtatc taatttgttg ttataatagc atggcaatta tgacaattca gtagaagagg
      361 taagtaaaaa aaaatgagca tagttaaagt acgaaatatt tttctcattg gtccgatggg
      421 tgctggtaaa agtactatag gacgtgcttt ggcaaaggag ctcaaactag aattctatga
      481 ctcagatgaa gtaattgaag agcgtgcggg cgcagatata tcgtggatct ttgatataga
      541 aggtgaggag ggatttaggc gccgagagca aaaggtcatt gatgaattga ctcaaaaaac
      601 caatatagta ttggcaacgg gtggtggagt cgtcattacg cctgaaaaca ggaatgccct
      661 ggcaggccgt ggcacagtga tttatctcaa aacatctttg caacagcaat ttgaaagaac
      721 aaagcgtgat accaaacgtc ctttattaca aacagaggat cttgagggac gcctggagtc
      781 actcagagat gagcgagaac ctttttatga tgaattggcg gatgtcagtt ttgaaacaga
      841 taaactgact gtaaaagctg ttgcaaacaa tataataaaa tacttatatg gcgaagtttg
      901 aactttatgc cgaggttgat gtttcgattt cagggcatca atatccaata attatttgcc
      961 gcaatggctt aattgatcct gatttaatta atcgttttat cacttcaaaa caggtgctta
     1021 ttgttactaa tcgaacagtg gcacctttgt atctaggtca tttgcaatct gttttaccat
     1081 caaaacaatg cgatgttgtt attctggaag acggtgaaga gcataaaaat cagcgcagtt
     1141 tatttactat ttatgattct ttgattcaaa acaaacatca tagagatacc tcaatcatcg
     1201 ctttgggcgg gggagtgatt ggtgatatgg ctggttttgc agcttctacg taccaacgag
     1261 gtgtgcgatt tattcagctg cctactacgt tactggctca ggttgatgct tcagttggtg
     1321 gtaaaacagc gattaatcat cctgccggca aaaatatgat tggcagtttt taccaacctc
     1381 aagcggtcat aattgattta aataccttaa aaacattacc tgagcgtgag tttcgtgcag
     1441 gtattgccga gatgataaaa tacgctttgt tggtcggtgg ctcttttttt gaacgaattc
     1501 aagacgttct gcaacaagga ttaacggtac atagtccgga attaccacta ttgatagcag
     1561 agtgttgtca agttaaagcg aaaattgtag agcaagatga aagggaatcc gggttgcgtg
     1621 ctttgcttaa tctggggcat acttttgctc atgcgctgga aacgtatacc gattataaaa
     1681 agtggttaca tggagaagcc gttgccatag gtttatattg tgcggctgta ttatcagaga
     1741 agaagggctt gctggataat ctaattgtag accaggtcga aaagatgctc atacatgctg
     1801 gtttgcctca taaaattcca aattccatag atttaattca attaagagag ttgatgagtt
     1861 tggataagaa aattaaaaat aattgtttac gtttcataat gattaaaaaa ccaggtgctt
     1921 gttatattga tgattcagtt actgaagatt gtttgcacaa tacactgatt aatgtggtag
     1981 aaggagaaca aaaatgatag atggaaagga gcctcttgat gagggagttg ccaatcagcc
     2041 cagaactcta ttcaaaccag gagcctggct tgcaaaagtt gatttcatta atcatttaat
     2101 tctctttaat aatgtactaa taaccatttt gtccgagaaa gagggcggaa aaacttgttt
     2161 taactctttg ttgaaaagcc atttgaacca acaaattaaa tcagtatcat ttaaggcaaa
     2221 accgccattt gacagtcaag atattatcaa tgagtttgcc aggcagttgc atcttaataa
     2281 taaggataac aatattggtt ctcttgcagc acaaattaat gaaagaaaaa cgcatgtttt
     2341 gctgattatt gatgatgcac atcacttgcc tgaaagtttt atcaaggaag tgatgattat
     2401 tataaaaaat caggaagatt ttggattttt ccatctttgt ttaatttctg attattctat
     2461 cgttgccaca ttgaataatc ttgctgttga gccgtttaat aatttaatac acacaataga
     2521 actcggggca ttaagcgaga ctgaaaccag aacttatgta ttacaacgag caatggcttc
     2581 tcatttaatc aacaggccac ttactgacac gcaatttaaa cagttctatc acttaaccaa
     2641 agggaactta tcaaaaatca attcaaacct ggaattattt ttatccaagt gcgctaatga
     2701 aaagcaaaat aataaaaagg caattttaaa aagaataagt ttagtcgcga gcttcgctat
     2761 tgttgcagga ttgtcctatt tttattttga taatgtctat aaagcacctg ataatactct
     2821 caaactgcca gatttaactt ctgtaacaac acttccaccc ataaatgacc ttataaaaac
     2881 tcccacacaa caagctgtcc ctaaacaaga atctgtaagc tatattgctt catgggaaga
     2941 ttcttcaacg aggcagctag tgcattatgg gttacccaaa aaacaaattt tagatgattt
     3001 tatggatgaa gaggatagtc ttaatacagt agctgttgta gataaggtcg ttgtcattcc
     3061 aaaactgaaa gctaaaaaca tttcagaaca ggttgggcaa gcggtagtta gtcagtcaaa
     3121 attacctcct attccccatt catcacaaac aaaattagcc agtgagcaac caggcatcaa
     3181 gaaaattaat agtaagctgt ttactataca gttggctgcc agtcataaag tggaggatat
     3241 caacaggttt aagaaccaaa acaaatggtt agctcaggca aaaataagac attttactaa
     3301 tgcgaaaggt agttggtaca tattaactat cggtgaatat gagagcagga atcaagctct
     3361 gctgaatata aaaaaactac caggcgaatt agccaaacta agtccatgga tacgatctgt
     3421 ggagggactt tcaaacattg gttagactaa gataatcagc ttggatatag ctgttagttg
     3481 ttgattgatt ctgggaaaat aggcttaaat ctgctaatct taacttagct atcagaattt
     3541 taaggagaaa aagatgtata aaagggtatt gtttgctact gactttgatg aggttggggt
     3601 gcgagcagct gagaaagcta aaaaaattgc tgacgagaat ggtgctgatt taattttagt
     3661 tcatgttgta gagcctattc cagcttatgc ttacccaggt tttgctggat ttgcagaagt
     3721 agaaatgtct attcgtgagc aagctgaaaa agagctgaat gaattggcag aaaaactggg
     3781 tgtagatgaa aagcatcgtt ttatagagta tggttcaact aaaaatgaaa tcctaagggt
     3841 agcaaaagaa cataaaattg atttaattgt gacaggaagt catggaaaac atggcctttc
     3901 tttattatta ggttcaaccg ctaatgcaat cttgcacggt gctgaatgcg acgtcttgat
     3961 tgtaagaccc aaaataagcc actaagagcg ccctctaact taagaaattc cgtactacct
     4021 ggtagtacgg ataatgtttt atagatttct caatatctca gaaaaccagt atactatgtg
     4081 ttttttattc ttctgattaa aacgattcat gaaattgtta cgcagttcta ctcattttgc
     4141 tatatttgat aagggtgcag ttgccactat tggcaatttt gatggcgtgc atttaggcca
     4201 tcagcatttg attaaggccc taagagcaaa ggctgatgaa atgaacttgc ctttagttat
     4261 tttattgttt gagcctcagc ctaaagagta ttttcacaga gagaaagcac cagcacgact
     4321 ttctaccttg agagaaaaaa tagatgtctt aaacttatgt caagtggatt acatctattg
     4381 catcaagttt gatgcaaggc tggctcaaac tcccgctttg tattttgctc agttttattt
     4441 atttgaagca ttgaaaatac gctaccttct ggttggccag gattttcgtt ttggtaaaaa
     4501 cagagaaggt gatgttaatt tactcaaaac actaggtgct aattattcct gtgaagtgac
     4561 agtgcaatct gattttctta tagaaaatga aaaaatcagt tcgacgagaa tcagagaagc
     4621 gttgcaacag gggaatttgc gtttcgcagc caagttgttg ggaagaccat acagcatgtg
     4681 tggacgtgtt atcaaaggag atggtcgtgg tcgccaatgg gggataccga cagccaattt
     4741 aggcattcat cgtttatcga tccctttgca aggtgtatat gctgtacaag tccatattaa
     4801 ttcgaatgtc ttttatgggg ttgcaaatat tggaaaacgg ccaacagtag ctggaactaa
     4861 aaatattttg gaggtccatt tatttgattt tcacgggtct ctatacggtc agttagtgca
     4921 ggtaattttc ttgcacaaac ttcgagatga agttaaattc tcaagtgtgg aagcgttaat
     4981 cacgcagatt aatagtgaca tcatattggc aaagacctat ttaaaacaga attggcttga
     5041 tagtgcgatt gattcatcga atgatgttgt gaatggaatc agtaataccg tttaatttac
     5101 ccttaatcgg ggtatttttt gtaagctccc ttcgggctgt ggagatgaaa cagaacaagg
     5161 gcatttaaaa ttatcaaaaa cttaatgatt tggcagagta ggcacctatg gcagaatata
     5221 aagatacttt aaaccttcca aacacttcat ttccgatgaa agcaagtttg tcagtgcgag
     5281 aacctgaaat gttggcagat tggcaagcaa agggaattta taagaaaatt cgtaaagctc
     5341 gtgatggaag taagcgcttt attttgcatg atggtccgcc ctatgccaac ggacatctcc
     5401 actgtggaca tgcgctcaat aaaattctca aagatattat tattaaatca aaaaccttta
     5461 gtggctttga tgcccccttt gtccccggtt gggattgtca tggcttacct atagaattga
     5521 atgtagaaaa aaaagtaggt aaagcaggga gcaagattag tccaagagaa tttcgtgcca
     5581 aatgtcgaga atacgcagca agtcaaattg atatacaaag agacgagttt caacgattgg
     5641 gagtcttggg agactggtat aacccttatg tgactatgga ttatcattat gaagccaata
     5701 tagtacgagc tttaggttta atgattaaaa atggtcattt acaacaaggc tttaaacccg
     5761 ttcattggtg tatagattgc ggatctgcgt tggctgaagc agaggtggat tatgaggata
     5821 aaacttctcc atccattgat gtggcgttct cagcagtaaa tccatccgag ttccttaatt
     5881 gttttgaaac tcagccagcg gtgaaaccat taattctgcc tatttggact acaacgccat
     5941 ggacattgcc tgctaacgaa gcggtatgtt tgcatcctga aattgattat gcattgattg
     6001 atgcaggtaa ttcttattat atcgtggcta ctgatttggt tgagtcggtt atggcgcgtt
     6061 atggaatcag ccattacaaa acatcgggct ccgctaaggg gcgagtcttt gagcatttta
     6121 aactgcagca cccattttat aagcgtcagg ttcccgtagt tcttgctgag catgtgacaa
     6181 cagagtctgg tacgggttgt gtgcatactg cacctgctca tggccctgat gactatttgg
     6241 taggtcagtt ctatcagttg ccattaataa atcctgtcat ggccaatgga tgttttgctg
     6301 agaatgtaga gttatttgca ggcatttctg tattgaaagc gaatgagact attctagcag
     6361 ttttgtcgga gcggaatgtt ttgttagcta gtgaatctat caggcacagt tatccgcatt
     6421 gttggcgtca taaatcgcca atgatatttc tggctacgcc ccagtggttt atctcaatgg
     6481 ataaatccaa tttgcgacaa gcaataatta acgagataga taaggttaac tgggttcctg
     6541 actggggtaa ggcgcgcatc agtaatatgg ttgaaaatag gcctgactgg tgtatatcaa
     6601 ggcagagatc ttggggaaca cccatgccat tgtttgtgca taaaaccacc agagaattac
     6661 atcctgacac tttggagtta atagagaggg ttgctatgat gattgagaaa tcaggcatag
     6721 acgcctggtt tgatctggat agctccgagt tgcttggcga tgatgccaaa cactatgata
     6781 aaatcacaga taccatggac gtttggctag attccggaat atcacattat agcgttttaa
     6841 aacataataa tgatttagat tttccggctg atgtctattt tgaaggatcc gaccagcaca
     6901 gaggttggtt taattcctca ttaacaactg cagttgcaat gtatggggtt gctccctaca
     6961 aaaccgtatt gactcatggg tatactgttg atgccgaggg aaaaaaacta tcaaagtcta
     7021 aaggaaatta tgttgcttta gataaattag tgaatcagca tggtgccgat attttaagat
     7081 tatgggtagc gtcaactgat tatcgtcatg aagtaagtat ttctgaggaa atcattaagc
     7141 gtaacgcgga tgcttatcga agaattcgta atacagcgcg atttttatta gctaatttat
     7201 ttgactttaa tccagccagt gattgtattg aggccaagga gttattagaa ctggacaggt
     7261 gggctctaaa gcgttgccaa cttttacaag aagaaataat tactgcctat gaaaattacc
     7321 acttccattt gatttaccag aaaatccata acttctgcgc agtggatatg ggtagttttt
     7381 atttggattt gataaaggac aggcaataca ccacggcaaa agacagtata gcgaggcgat
     7441 cttgccaaac agcgatgtat catatggtca aagcattcac tatctggttg gcgcctatct
     7501 tatcttttac agctgaggaa atatggcaaa caatacctgg gaataatagc gaatctattt
     7561 ttattgagca ttggtataat gcctggccaa ctattgacgc agtgaacatg gaggattggg
     7621 aacagctgca catcgttcgt gatgaagtaa acaaagcttt agaagaaacc aggcaaagag
     7681 gtgaaattgg ttctgcattg gcggctgagg taactgttta tgctgatgcc aaggtattac
     7741 caaaattaac gcgtttaggc gaagagttac gctttttatt cattacatca gaagcgaaag
     7801 cttgcccaat tagtcaatcc cccaaaggat tagctgttac tgattgtggg gtatcaatac
     7861 aggttacagc cagcgttcac gaaaaatgtg ctcgatgttg gcatagacgg gaagatgttg
     7921 gtcaaaatca agagcatcct gaactgtgct tgcgctgtgt aggaaatatc agcggatatc
     7981 atgaagagag gctttacata tgaaaaaatg gccttggctt gtacttagtt ttttagtaat
     8041 tatttgcgat cagttaagta agtattgggt tggggtgtta ttaactccat ataaacccat
     8101 gccagttatg cctatgttga atttcacttt agcttttaac acaggcgctg cttttagttt
     8161 tttaagcggc gcaggcgatt ggcataggtg gttttttgca gggtttagtt ttttaatgag
     8221 tattattttg ctgatctggt tagtacgtac tccagaacag gcacgatttc aatctgttgg
     8281 gataagtctt attctaggag gcgctattgg caacttgatt gacagaggtc ttcatggtta
     8341 tgtgattgat tttattgacg tttattataa acaccaccat tttgccacgt tcaacttggc
     8401 cgatagcgct atatgtatag gagccgcaat tttagttctg gatttattga ttcgtaggga
     8461 atagactcaa actgcctaga aaataatgtc ataagttagt gtcctcatgt ttgactatcc
     8521 tctaacagta gggtttgcgt tctggttaga tgcaaataat actgaaaaag cacagaatag
     8581 atggctgttt ttgttcaaga tgggcgaaaa aagactttct cggcaggagg ctgatctctt
     8641 acggaaataa tagctccgaa gtggtttagt atcttaaccc ttttcaaagt caaaatacta
     8701 taccaaagct tgtcatgcat taatggaaaa caatgcttac caattagaat cgatttattt
     8761 tcagataatt gattgtgcta acctggctaa tccattcagt tgcttatggt cagggttatg
     8821 aataattact gctgctacag ctggcaagtt taggaaaagc tcagcttcat tccagtcaat
     8881 ttcagctggg aacagattta atcctggtaa atatttgctt agagcattac aaaatgtcag
     8941 attggcgggg tagggtcgca cttgtttaaa tccaatattc gctgcggcca tagcttctga
     9001 tatggtagct acacttggaa aatagcacat cgaataaaca cttgcagttt gggctataga
     9061 agctaaaaag ccggggctag aggcaaaatg tacacccgcc tggtagcatt gctctaattg
     9121 ttgagtatca attatgccgc cggctccaat ttttagagat gggtattggg ttttaatctt
     9181 taccaataaa ttggtatcgg acgagttgat ctctactaaa tccaaatcgg aatttgcaat
     9241 ttgatcgagt ctgtcaaata aaaaattatc gatatccaat gatatgatca cagattgatt
     9301 acctaagatt ttttcaataa tcatgtttta ttccgaaaat tctgcaataa tctttcaatg
     9361 ttagaagact tgctgattgt tggcaagggt attgataaga aaaacaacaa tttgtttgat
     9421 gatgaggtgg gggtagagca ccctgaatta tgattctcag acaataatcc agggtaggga
     9481 gatgaaatat aattctacct tatgatgtct tgaatggaga taaagacgga gtgggattaa
     9541 atcgttgttc aacaacctca tctgttttgc ttcttataga agtcacaccg ggttttgtag
     9601 tgtctacccc aaatctctcc aggttgcgca acatagcttg cattatttgg aacggaactg
     9661 ggccagtgcg tggcatctga gtgagtgcgc ttataaaata gttgcgtgct gccccttcag
     9721 gaatttgact ttggaattgt cgcagctcat cacgagtcat ccctgggcta ttcatttcct
     9781 tcatttcttg tattttggtt ctgaagaagg tagtgactgc agcaagactt ggtcctggtt
     9841 ttggtgaaag agtcgtgcca cctcctccca atggagtcaa agttactgac tcttgattga
     9901 tgttagattg gtttagttca tttaaagcag taccaactgt agattgaagt ttacttatgt
     9961 tattagcaat ctctattttt tcttttccat tgtcttctaa ttgttgtttc gtctcttcaa
    10021 ttcgttcttt atgaatggtt gtttctaaac ctttattatg atgtactaat ttcttgactg
    10081 tcattggtgt ttcatactga agtttatgaa aatcatgctc agcttttttt tgtttctccg
    10141 gactatcttt gatagattcc cagtcttcac cttgttttaa aagataaaat ttgccgtctt
    10201 tttcaagtat tttttgttct ttatggatag gatagaattt accgcctaaa ttaaacagtt
    10261 gttgatcttt tcctatcacg taatgcgcat catttaatga tgttactttc tctcctttct
    10321 cattaaaaaa ggatttttct tttctatgta ctgcaagcat gtcttgtaga tttgccaatt
    10381 ttaaatttaa tccattatgt ttatgcagca atttggtggc ttcctcattt tcttttggat
    10441 ctgtgctcat aataagatca tttattgttt ccaattgttt ttccatttcc aaactaagct
    10501 tatgaattag agcttccatc ttgatggttg caagatcatg tttagattgg ctatagtctt
    10561 tttgggcttt ttctaatgct gcgggatcat ttttaatggc atcccattgg cctttttgca
    10621 ggagatagaa ttgtccttta tgttctacta cttcctggtc tttattaata gccaggtgcg
    10681 cgtcttttaa tgagctgacc gggtttcctt tttcgttaac ataggatttt ttacctgtat
    10741 gagcgtcatg taaaacttgc aggttagcca gttttgcttt tatgtcatga tgctcacgct
    10801 gtaaggattg aacggttttc tcatcgcctt tatgcttttc catcagttgc gtgagtttct
    10861 cactgtcttt gctgaaggct ttcatttttt cattaatttc ggatagtgat aactcttcag
    10921 agtcagtgag aagtttttca aatccttcca aattttcttc ataggttttg tacttgtctt
    10981 caagatcttt gccttgacgt tccagcttat ccagtttttt ttctaattct tcgcctttct
    11041 taatattttc ttgagcacgt ttaatagctt gttcatattc atccagcatt ttttgaattt
    11101 ctttatcagc ttgtgaagtg gatggagtgg actgttggct tggcttgctt gctttttcag
    11161 cattttttat ggctttttca ttgtattctc gaataatttc cttgactttt tccgcagcat
    11221 gggatttttt ggataaatac cacaaaaaca aagcggccat taatcggcgt ttaaagagct
    11281 catgctccat tctgtcttgt tgattttgta gattgatgtt tctttgcaga gccatttggg
    11341 cacctaactc agctattacg tctttacccg ccggagataa aagaaatttt ttgacctggg
    11401 atgcgttttt taacccttct cggcccagat taatggtgga acctttgtct tccttagcct
    11461 ggggatcaag caagctggat tctatccatt tctcaaaaga agttttaaag ggcttggctg
    11521 gctcattggg ttgagattct tttttagtgg tgggcattga tagcttatct tcttttttac
    11581 tatcctcaac acgcattttt tctacaacag gttccttttc cttggttttt acggattcaa
    11641 gactcccttc tgatgtttta acttgatgtg atttgttatc ttttgccata aataggatcc
    11701 ttaatcaatc gcccccagtt atcttatatt atacacaatg ttgaccaata aagcatcagt
    11761 ttattcatgt tttaacagga ttatgccatt tactatcgtg cgatcttgag tttgcaactc
    11821 atgcatgatt ggagtctttt tttgctgaat attagtattt catcttagaa gcaagtagca
    11881 taatttgcaa ataaaaacca tgaaatcaat gcaattagat ttggaagtgt ttagatagtc
    11941 agtgtatgtt cttgaggcat tgtgtctgat gtttgtgtgg caccatgaaa cagacctgga
    12001 aaatgcagtt gttgggattt ctgattatct ttgactttat tggtggagct cttttcgtcc
    12061 aataataaat tacgtaacag gcagtgactg tctgagtgag gaattaattc gcaacgtctg
    12121 ggatcattga aatagtattg attatcctgc actattttat gtgtcagtat atcctcatca
    12181 tggaatgccc attgatatac ttttccacga tttatttcta ttaaggaaag caaatattgc
    12241 tttaattcac ccgctttgct gtctatgtgg ttacataata tagtcacccc atacgcgctt
    12301 gttgagaaca aaactcctgc tgaatgatcc caaatctgtc cgaagtgata aaggatagta
    12361 ccggtagaat aggcaagaag tcgggaaata ggattaataa tagataaatt tactcttatt
    12421 gttagtgaaa gtatgtctat cagagggaga atcaaaatat ttttaatcag ctgaaggagg
    12481 ggtaaaaaaa gtagccttat gacattaatg cctatgtttg tgactggatt atctgaaaaa
    12541 tggccaaagg gggaagtaaa tccataaaga ggggaaagcg taaattcaga tatgaagtta
    12601 tttgttgatg gacgctctgc tatatcgatg agtaatttcc caatcacact gaagttacct
    12661 aatacagaca gtaatttgcg gaaaaaattg gtaattcctc taaaagggac gtgtattaaa
    12721 agcgaactag cctcaagtaa ggggatggtt aaaatagcta aaataagatc aaaacttgcg
    12781 gctaaagttt gtttacccag ttgttttatc aatttgccac tttttttcca gccatttata
    12841 aatccatcaa aaaaaccata gtaaaaacct tcaaaaaaag gtgcaaccaa taattttcca
    12901 atatttataa tattcttgca aaaccacttg caagtcccaa ggaaccagtc atcacccggg
    12961 tgcataatgg tatcatagat agcggcacct cctttacctc cgagagcagc ataattggca
    13021 taaggaaagt cacccatttc atgttgcagt gaaggaatcg cttttgttaa accataacca
    13081 aggctaagcg ctaaagaaac aataatagca atttctgctg ggtcttcttt caagacagag
    13141 acagcatcga caaagaactt atctaaatta ccgctcacta ccataccttg ccagagagtt
    13201 gcagaagcag agatagcttc tgaaatcttg ccatgattca tcaggcgagc taatttttga
    13261 acaggttcaa ttccataaat caagccactt aagtgcagct tttttaaaat gtcagtgaga
    13321 acattgggtg cgagtacagc acctgcgcca tagacataag ctgccatggc aagagttcca
    13381 atcatggggt tacgctcgct ggtgtctata aaaaatccag ctaaatggcg tactacgccc
    13441 aaagctctgt gcaaaggatt ttcataggtg tgcacagtag ggggatgatt cgctcttgca
    13501 atgacatagg ggctgttgtt gatctgatca ctaatgaact cttcaagagt ttgtccattt
    13561 gcgagaagtc cctgattttg gagcgcttca tgcagagagg ttcttttaat ttttttggat
    13621 tttggacgct gatctctttc tgaaagatgt tgtttagggg catgagttgc atgcacatga
    13681 ttaaaaaatt cttcatgcag gtcatgtatg atgaattggc tcatgctttt gtaaaaacct
    13741 ttaaaaaaat caggtatacg ggaaataaaa gctttgaaac ggaggctgat tctttcaggc
    13801 aagtaaagat gccttttgct gatttggctt aaaatatata aatcctctcc atccggcaaa
    13861 ggataaaaga gttgatcatg tataaaatat ttgagatgaa tcagtttatt ttcgagtctt
    13921 aacagaacag tatttttttg aggcacaata acccaattac cattaggtaa gtggcttagt
    13981 aatcttttcg cctcttttaa actgtttacc tctgtctgtt gggctgagtt ttccactaga
    14041 tgatttacca tcgcagccag actattataa cctatttgag gcagtccttt gagtttgtag
    14101 taaggcgtta ttttggtaat agatactcca tcagagcgtt tttgtatttc agttttatga
    14161 cttggttttg ttgtgactgc cagataatta atccaatcgt gaccgtcatg ccaaagttta
    14221 cttatgaatc gttccagttt tgcaaagcga ttggccggga tgttgtttaa tgctggcgca
    14281 gggcagagtt ggtattgctg gcaaattacc ctgagcgcat cgtcaagctc acggctactg
    14341 tgcccaatat ggatttcact atcccaaaca aaacaaggag taattggttt tccaagttgt
    14401 gttttagtca ctttggtaaa gtccatttga tcctggcgag aaacagaaaa aactcggtta
    14461 aaccgataat gctgtgctgt tttgtgcgca tcatatagca tataaccaaa acttccttct
    14521 gaaaccacag gattattggc tatcattcga ttgtcgttct ggcaatgttc aataatggaa
    14581 atgatttttc ttcttaactc ctcctgtcca gcatgatcat gaggttttaa ttgtctttct
    14641 tttttcaata gatggtttaa ggaacggata caagcattag ccaggttaat tttttcttcc
    14701 tgctcatggc ttaggggttt tttatagctt tcagcttgaa ctttatagcg attgacgtga
    14761 tttataacat cagcaactag cgaggatggt ctgtctcccg gaagaatttt taacagttca
    14821 gggttatgag tgaagagcat cagctgaact gttttcttgt tgtgattggc tattaagcgt
    14881 agtctgtctc ctccaggaag gagatattcc atattataat taccataaaa tactgtaccg
    14941 gcttcgtgac tgaaaatact tccggtgata gttggcatcg ctttgtgtgt gtgcggagta
    15001 actctgagtg gaataataac ttgagtttgg ccatcagtag tttcaaaatc aggcataaag
    15061 atttatttgg tttaatatat ggtaaaagga tactgtcatt aatttagtga ttttttatcg
    15121 cttaagttgg ttttgaagaa aagtaaagcc catttaagct aaaataatac aaaatcgttt
    15181 aatttaatgc cattaatgca cagggtactc gaattggaac agaaatagca agcctattta
    15241 atgaaaaatt tggtatctct ggaagtttta tgacatggct gttagaatgt tttaaaaata
    15301 aacaggaatc aagattaatg agtagttatt gtggttatat tgctttagtt ggcaggccaa
    15361 atgttggcaa atcaacctta ttgaatcgta ttttacagca aaaattaagt attacctcga
    15421 gaaaaccaca aaccacgcgc catagtattt taggaatccg tacagaagat gaatttcagt
    15481 ttgtttatgt agatactcct ggtattcatc agggtaatgc gaaagcaatt aatcgcatga
    15541 tgaataaaac agcgattagt gtactacgtg acgtggatgt gattgccttt ctagttgatg
    15601 gaacgcattg gaaagacgaa gatgagtatg tattgaattt aatcaaacaa gctaaagttc
    15661 cttgcatcct ggtagttaac aaagttgaca agataactga taaaacacaa cttttgcctt
    15721 ggatagagca aataagtcag cgatatcaat ttgcagccat tattccttta tcggcgaaaa
    15781 ctggtttgca ggtggatgaa ttggaaggca aattaaagac ttatttgcca gaaggaccac
    15841 atttgtttcc tgatgatcaa ttcaccgatc ggtctataaa atttttatgt gccgaattgc
    15901 tccgtgaaaa aattttccgc ttttgtggac aagaattgcc ttattctgta acagtagata
    15961 tcgaatcatt taaggatgag ggaaatttaa ttcgtattca tgccttgatt ttagtagaca
    16021 aggataatca caagcgcatg atcattggtg ataaaggcca aaaattaaaa gaaatggcaa
    16081 ctaatgcccg tttggacatg gaaaaaatgc tagataaaaa ggttttttta caatgttggt
    16141 gtaaagtgaa atcaggctgg tcagatgatg agcgaatact gaagcaattg ggatatgacc
    16201 agtaaatcgc tgaatgcttg ggtaattcat aagcaatggt ctggagatac cagtgccaga
    16261 ctaaagcttt ttaccaagga actgggatta attaattgct tgtgcaaggg agggcgaact
    16321 cccaagaaac agtcattact tcaagctttt attcctctgt gggttagtat cgaagagcgt
    16381 tatgatcaat attacacacg aaacattgaa agcacttctt ccagactgga tcttgaagga
    16441 cattctcttt tttcaggcct gtacatcaat gagttactct attatacgct gagcccggat
    16501 ttccctgacc cggatttatt tgatgcttac ttgttcacct taaatggcat agcattagct
    16561 agagaaagag aggcgataga agctttgctg cgtcgctttg aatgggcttt gcttaaggct
    16621 tgtggataca cattttcatt tcttcatgag gcgcgaacag gagagttaat tgttcccgat
    16681 tctcattacc aatttgtagc tggggagggc tttattttag gtggtgataa aaaaatacct
    16741 ggtgagcatt tacttgctat agcagcagat aatttaagcg agtccgctta tttaaaatca
    16801 gcgaaattca ttatgcgtca ggcaatcgat catcttttgg ggggacggga aatcaaggcc
    16861 cggtccttgt atggaccggc ctgagatagt ggtatgatcg ccaagcgctt ctatgctatc
    16921 acatcaggtc cagggtttat tatgttaggt gaggtctgga tagattttcg atttcttcca
    16981 caggattttg ctcctgaatt ttaataaaac caatttgctc ttttaaagta cctttaggat
    17041 tgagtttact tcccaatgaa atagcaagat tatctaatct atcaaccaaa gctcgtgagg
    17101 tagtgtaatt accagcatca atctctttga caatgtcatc aactactttg acatggtgtc
    17161 tattccaatg aaaagtgaaa attcgtctga atttggccca ccaggtgtcg cctcctttag
    17221 tataatcatc aagaacagtt ctaatggcat gtatcttttt gtattctaca tcatttctgt
    17281 ggagctgatg gcgaggatca ttcataattt cttgaaaagc atttcgccat aattcatgat
    17341 aagcagcaaa atttccgggg aacgttgcag aaacagacct tcttgtatca gggaattggc
    17401 tttctgggcg tacttcttgt cttccagttt gtgaaggtgt accagtatag ggagtgagct
    17461 ggcctgttct ggttgaagta agactctcgt caagagctga ctgataattc actgtggcag
    17521 gaaaaaggct gcgattataa gcaaattgtt ctttgggaat cgccaatgct tgttcaggag
    17581 ggtgtcccat gccaacggcc cagggatcaa cgattagcgt accagttgga aaaacttcag
    17641 cggttaattg ctgtgggggt tgagggagtc tgcttccttt aggaacgagt aaggtaatat
    17701 tatgtagata ccggccgtta ctgtgcatac ttcctataac ggcaacatca tactgagttc
    17761 ttatttgtgg atcatctaac aatactcctg cagcgtggta agcaaaattg ctgcattcag
    17821 agacacgaac tgcctcgcca tcaattttaa aacttgattt tttatttctt tgctgggcaa
    17881 atttaatata ttcctgagcc gatttagggg ttaagagctg gggagctccc cattgactaa
    17941 aaggtggtgt tctggataag gagtttgtac tgtatttttg gtatctttgt ccgctagcta
    18001 tgccttcagg agtctgagca aggcaatgtg gaaaattccc aatatcagca acaacaaatt
    18061 tttgatattg ttcttgcttc agtctctctt gtatctgttg gggggcaata gccataaatt
    18121 actccgataa ctgatttgag tttattgtgg cggttatttc ttaaccatga tttctttaaa
    18181 aatatagtgc ttttattttt atggatatga ataaaaaatt acctcttggg ttgttatttt
    18241 tactgaatct cgattatgat aattgtgatt tcataattaa ttcggttttt tatttaagcc
    18301 tttatcgctt tctgcggttt cttgacaagg tgacatacaa ctgagaaaag taaatttttg
    18361 caaaaactta aatacactat gggagtttgc cgatttttta gaataattta gttcatctgt
    18421 ttcatgcact tcaattggat tgccgattaa atcgaggtcc gttatcctgg tattggtttc
    18481 aagtattttt ttacctgctg ctgtaatttg attactaccc agatgaaggg tactcaaaga
    18541 cgtgttatta gcaaaatgga gtgttgattt atcggttatc gcgttttttc ccaaaataag
    18601 taaagtcagt tgattgtttt tagctaattc aatagctcca atatcagaga catcacaagc
    18661 gcttgcaatc agctctttta tattgatatt acttgctaat gctataaccc cctcatcttc
    18721 cagactattg aagcatagat ctattttctt aagattggat tgagcaagat agcctgcacc
    18781 tactttggtt atcttattat aaagtgcata aagggttatc agtttggcgt tagcggctag
    18841 agctttaatt ccctcgtcac caatctcgtt aaaactgaca tctaacgtag taatgtggtt
    18901 atcttctgat agccattgag caccctccgg accaatatta ttatggctaa tgttaaggga
    18961 tgaaaccgat gttttattaa caaaaagctt cataccattg gctgtaatat cattatgtga
    19021 caggtcaaga ctggttatgg caggatgatt ttgaagaaac tcacatagca gtggtatgtc
    19081 attatctcta agatttaaaa aggataatga gagttgatta agatttttat catagccttt
    19141 ttccaaatcc gataatgtta acaccaaaca ccatgttcaa ttataattaa aattgtttaa
    19201 tattttactt aaacaagaaa atcatatcaa gctttttgtt cagtttatga tgattcaggg
    19261 aaatgtgatc agtgcctatt attcaggcat cagtttgact tcaatattat aatttcttta
    19321 aagtggtagt gaataaagta aagtatggtt aaatgcttta ggtagtgccg tttaaactga
    19381 ttgaaacaag gtaacgtgaa tagcagtttg tcaagaactg cattatactc tgcattttat
    19441 attttttggt gcaaaatgaa taaagagtta ttgttaggtg ttaatattga tcatatagca
    19501 accataagac aagcacgagg aactcgttat cctgatcctg tccaggcagc aatggatgct
    19561 gaagaagctg gggcagatgg aattactttg catatgagag aggacttacg acatattcag
    19621 gcgcgtgatg tcagattgat taaacaggtt ctgcaaacac gtatgaatct tgagctggcg
    19681 gttactgaag ccatgcttga ttttgccgag gaaatatcgc cggagcatgc ttgcttagtg
    19741 cctgaaaagc gggaagaact aactacagaa ggtggattgg atattttgac tcaccgaaat
    19801 gtggtggaaa aggcagtacg tcgcttacaa ttaatgggaa gtgaggtttc tttatttatt
    19861 gacccggata aagagcaaat aagagcggcg gttgatgttg gggcgccagt tattgaattg
    19921 cacacggggt gttatgctga tgctacctca gaggaaaagc agcattatga attgcaacga
    19981 attaaagagg cagctgaatt tgctgccagt ttgaacttgg tagtgaatgc cggtcatggg
    20041 ttgcactatc ataatgttaa accgattgca gcgattaggg agttaaatga acttaacata
    20101 ggacatgcca ttattgccag agcattattc tgtggattaa aagaggcagt aagacacatg
    20161 cgccaattaa tgcaggaagc gagactttat gtcaacgact gatgtcattg ttgtacgcat
    20221 cactggcgat tctggtgacg gagtacaatt ggtaggtgaa caattaacca ttagcgcggc
    20281 attgacagga cgtgatgtgc gtactatgcc tgatttcccg gcagaaatcc gggctcctgc
    20341 tggaacagtt gcgggtgtct caggatttca gttggcaatg gcggaaagag ctatttttac
    20401 tgccggagag tcattggatg ttttagtggc tttaaatcca gctgcattaa aaaactcact
    20461 tcaacatttg aatcaaggtg gtttgctgat cattaatgaa gacagttttc aagaaagaga
    20521 ttggcaaaaa gcaggtatcg ataagagttt cctggataat tgtcgagaac attatcacat
    20581 tgttgctttc ccattaatta cacaaactat tcaggcagtt gaatccatta accttacaaa
    20641 accacaggct gtcaaaacca agaattttta tattttggga ttggttttgt ggttgtttga
    20701 tttgcctaca gaaacttgcc aggcttttat cgctaaaaag tttaaaacaa atcaggccat
    20761 agcaaaagcc aatgaattgg ctttgttggc aggatataat tatgctatga ctctggagct
    20821 ttcccgtcgg gattacatgc tgggggaagt caacagacat tcaggtgagt acaggcaaat
    20881 cactggagtt gaggcagtag ggttagctct ggctacatta gctactcaga cacaagttcc
    20941 tctgttggtg tcaggttacc caattacacc ggcttctgct attttgcatg aatgtgttcg
    21001 cttatctgat tacggcgtac agttaataca agctgaggat gaaattgccg ctatttgttc
    21061 ttgtataggg gctgcttatg gtggtcgttt ggctctgacc tgtacttcag ggcctgggct
    21121 tgatttaaaa tgtgaaagcc tgggcttggc tgttgtgacg gagctgccct tggtgctagt
    21181 tgatgtgcaa agagcggggg cttcaacagg tttgcccaca aaaaccagcc agagtgattt
    21241 aaaacaagct ttgtatggtc gccatggcga ggcaccttta cctgtgattg cagctcaatc
    21301 ccctgctgat tgttttaata caattattga agccttcagg atagccatca agtacatgac
    21361 tccagttatt gtattactcg atgcttattt ggcgaacgcg gcagagccct gggaaattcc
    21421 tgctttggat tctctcaata taccaaaacc ggagtttaat cgttttacca aaccctatca
    21481 tcgagatgaa tttttaagta gaagctggaa tgttcctgga acttctgggt ttattcatca
    21541 aattggaggc ttggaaaagc aaggcgatga aggacgagtt agttatgatg ctgagaatca
    21601 tcaaaaaatg gtgattacac gtgccgagaa aataaagggt attgcaagag attatcctgc
    21661 tttggttctt gagggtgata tgacggctcc cacgcttgtt ataggctggg gtagtacttt
    21721 tggtagttta aagtctgctg tacatcaatg tcttgaacag ggattaccgg ttgcctatct
    21781 gcatctgcgc catcttaacc cattacctga tgatttgggt tctttattga aaaaatatga
    21841 caaggtattg gttgctgaat tgaattctgg ccaattatgt caactcatca gagcagttta
    21901 tctggtggat gcgcaatcaa ttagccaatg caatggtcaa cctttcagtg ttaatcattt
    21961 ggtaagtgcc attaaagcgg aagccaacta tgaacagcaa ttataagcgt gaagattttg
    22021 tgaatgaaac agaagttcgc tggtgtcctg gatgtggtga ttatgccatt ttggctgctt
    22081 tgcaaagagt acttcctgaa ttaggtttgc ctcctgagca acacgtcttt gtctcaggca
    22141 tagggtgtgc cggacggctt ccttattata tgaacactta tggatttcat actatccatg
    22201 gcagggctac agcagtagca actggcttaa aggcaatgcg agaggattta tgtgtctggg
    22261 ttatcaccgg agatggggat gcgctaagta tcggcggcaa tcatttactg catattttaa
    22321 ggcgcaatgt caatgtcaat atacttctgt ttaataatca ggtttacgga ttaaccaagg
    22381 ggcaattttc tccaacgtct cagaaagggc aagtaactaa aacatccccc aaaggagtga
    22441 ctagtgagcc aattaatcca atcatgatcg ctttggcttc cggagcaagt tttgtagcca
    22501 gagcagttga taaagatcct gtccatttgg cttctgtgct taaaaaagct tatgaacata
    22561 agggatgctc ttttgtcgaa atatatcaag attgcaatat ttttaatcat ggtgcttttg
    22621 atgattttgc agttaaaaat aaccgggctg aaaacacaat aatattggaa gacggtcagc
    22681 cactcgtatt tggcgctcaa aaagaaaaag cgctgcagtt ggatgatgag caatttgtac
    22741 aaatttcagt cgatgaaaaa gcgctttata agcatgatgc gaaaagctta attagcgcca
    22801 tgcgtttagc tcgattgacg ttccctgatt acccagtgcc tttgggagtc tattatcaaa
    22861 aggatcgtga gctttttact ctgagccagg aagtgaagaa aaggccggaa gatttgccag
    22921 aactttttaa agccaaggct agttggaaac aaaattaatt tcacattgat taataattcc
    22981 tctggatttt gcaatgtaat tattcttcag ataactgctc aaaaattgaa agggggcagt
    23041 tgtttcagtt aattactttt tgagatactc tctgatattc ccttaacaat tgatccataa
    23101 aaaatcccgg catacagccg ggactcaggt agaggagacg atattaaatt attcgcgacc
    23161 tgagaatgcg cttaacaggt tcagcaggct aacaaataag ttataaatgg aaacaaacaa
    23221 accaatagtg gccattatgt aattggtttc tccttcatga attatggcgc tggtttgtaa
    23281 tagaatcaat ccagatgaaa ttaatacaaa agcagcagaa atcactaatt gcaaagctgg
    23341 tatttgtata aatattcccg ctatcatcgc caataaggca accataacgc caacaaaaag
    23401 aaatccaccc aggtagctaa aatctttttt agttgtcagg gcatatcctg atagagcgaa
    23461 aaagatcatt ccagtaccac ctaaagcagt agcgactatt tgtgggccat ttgaaaaacc
    23521 tgtgatgtaa taattgagta atgggccaat ggtatatcct aaaaatcctg taaaagcaaa
    23581 gacgcttacc aaaccccaaa cgctatttct taaagcttga gttagaaaca tcaaaccata
    23641 aacgccaact atcattaata gtggattcat tgggcgagct ccactaataa aggctacgta
    23701 ggcagttaat gcgctaaata tgaaggttaa acttagcagt aaatacgtat ttcgcaggac
    23761 cttattggtt gcgagcacag attctctttg ttgactaagt atggtgatat catttcggtt
    23821 cattgctcag aactccagtt atttattatc agtgtagcat atctatggtc aaatttccta
    23881 tttttcaagt cgtatcactt gaaaattttg taataaaatt tacatatagg ccaatagaaa
    23941 ctttattatt aaggcgaaaa ggttttcgtg tcaattttgt tttgggctta tatggaacga
    24001 tatagtaaga tgaaaaatat gaatgtcatt taggaaaaag gatgaatcga gtagcgcttg
    24061 tgcaaatggt atcatctgcc aaaatagcag ataatttgca actcactgaa caatatttaa
    24121 ttcaagccag ggaacaggaa gccagtttgg ttgtgctacc tgagaatttt gcttttatgg
    24181 gaatgaatga acgagagaag ttgcacatcg ccgaatatta tggtcaagga cctatacaac
    24241 aaagaataag tgagttagcg agagatttgc gggtatggat aatagcagga acaatcccgt
    24301 tgaaaagtat gggttcaaag gtaagagcaa gttgtatagt ttatgatgat aaaggcctaa
    24361 atgtcgctcg ctatgataaa attcatttat ttgatgtcat agtttccgag caggaaaaac
    24421 accaggaatc gttaactata gaggcaggaa aagatatagc gctagtggat acgccagtgg
    24481 ggaaaattgg gttaaccatc tgttatgact tgcgttttcc ggaattgtac caatatttaa
    24541 cccaaagagg cgcacaatta ctttcagttc cgtctgcttt tactgcggta actggagctg
    24601 cgcattggga ggtcttactc agagcaagag caatcgaaaa tttatgttat gtcttagctc
    24661 ctaatcaggg tgggactcat gaaaacgggc gtcatactta tgggcatagt atggtggtgg
    24721 aaccatgggg taaagtgttg gctcaaaaag aagaagggca gggagtaatt ctggctgata
    24781 ttgatttaga gagattgtca cagttgagaa gacaatttcc gtgtgttgaa catcatgttt
    24841 taatgtaaaa ggtatataaa tgacaaaatc tcttgcgata gcaaaaaata ttttattgac
    24901 accagcttcc ctggatgaag ccggcataga aaaattattc aagaccatgt ttaatcggaa
    24961 cgtcgatgat gctgatcttt atttccaaag ttgtagttat gaatcctggt atttggaaga
    25021 ttcagaggtt aaaagcggta atttttctct ggataagggg gtagggatta gggctgtaag
    25081 cggtgataaa accggctttg cttattgtga tgatatattg ctgccttcca tgcttcgtgc
    25141 tgccgatgcg gcgcgctcca tagccctttc tggttcaaaa acagtaccgg ccgtcaaggt
    25201 attaaaggct tctgtaaatc ggtatgagag tttaaatcct ttggaaggaa tgagcaaaca
    25261 ggagaaaatc gctttattgg aagccattga taaagaggcc cgtagtcttg atcctcgagt
    25321 gatacaggtt aatgccactt taactggttg ctatgaagta gtaatggttg ccaatatgga
    25381 tgggcagatg atggctgatg tgaggccatt ggttagcatt aatgtcagtg ttattgttga
    25441 agacagtcat ggtagaagag aatcggcgag atcaggcggt ggtggacgag tagcttattc
    25501 ctattttacc gagaaagaaa acgccttgag ttatgcgcgt gaagcggtac gggaagcatt
    25561 aaccaatttg aaggcagaac cagctcctgc tggaaccatg ccagttgttt tgggcccagg
    25621 atggccaggg gtacttcttc atgaagccgt tggacatggt ttggagggtg attttaatcg
    25681 caagggatta tctgcttttt cagggagact gggtcagcag gttgctgctg caggcgttac
    25741 agttgtagac gatggcactt tagagaatcg acgtggctct ctgactatag acgacgaagg
    25801 gacgccatcg caatgcacga ctttaattga taatggaatt ttagtgaatt acatgcagga
    25861 caagttgaat gccaagctta tgggaatgca atcaacaggt aattgtcgtc gtgaatccta
    25921 tgctcatgtc ccaatgccga gaatgaccaa cacttatatg ctgtcaggac agtacgaccc
    25981 tcaggaaatt attagcactg taaaaaaagg attgtatgcg gtgaattttg gaggcggaca
    26041 agttgatatt acttctggcc agtttgtatt ttctgcgagt gaggcttatt taatagagaa
    26101 tggcaagatt acaaaacctg tgaaaggagc cactttaatt ggcaatggcc ctgatgtgat
    26161 gaaaaaaata agtatgattg gtaatgattt ggcattggat agagggattg gtgtatgtgg
    26221 aaaagaagga caatctgttc ccgttggcgt gggacaaccc actttgaaaa ttgatgcctt
    26281 gacggttggc ggaaccaagt aagaaaatgt cagtttagca ttgcgctaca tttggttttt
    26341 ggatttagtg acatccggtg ttaaaaaaca tgaaattgat tgatttaacg agtgtgtacc
    26401 caactatttc caggagttat tcttgacaaa tcaataccct aaaatcggtt tggctttagg
    26461 tagtggatca gcgcgaggct gggctcatat cggggtaatt caatcactca aacgattggg
    26521 ggtaaggatt gattgtgtcg ctggatgttc cataggtgca ttggttggcg ccatttatgc
    26581 ttgtggcacc ctggatctgt ttgaacaatg ggtgttaaag cttaataaaa gggaaatggc
    26641 taaactcatt gatgtgaatt ttttaggtgg cggggttctc tctggtagta agttgatgac
    26701 ctttttcaag gattttggtc ttgaaaaaaa tattgaggag ctctcgctac cttttagttc
    26761 agttgctact gatttgagta ctggacgtga gatatggcta agtaaagggt ctcttgagct
    26821 tgctattcgt tcatccatgt ctttaccagg gttatttacc ccgcagcgtt ataaggattg
    26881 ctggttagtc gatggtggct tggttaatcc agttcctatt acattgtgtc gagctatggg
    26941 ggcagatgtt gtaatcgctg tcaatttaaa tcatgacttg ctaagagtac ctccgagcac
    27001 gacagaacac ccggggtttt tttcagttct ctcaggccaa tttgataaaa tgataaaaag
    27061 aataatggga atcagtgatg ggccaggata ttatgatgtt ttgaccagaa gcatcaaaat
    27121 aatgcaggac cggataaccc gagcaagagc ggcaggcgat cctgctgatt tgactttggt
    27181 tcctcaatta ggaaacatca tgctttatga ttttcataaa gcaaaagagg cgattcatga
    27241 aggtgaaaat tgtgttttgc gagaaaaaaa taatatttta caactagttg gtctagaata
    27301 gtaattaaac aagcttaagg aagagccatg caaaaagaat ccaattccaa ctcattattt
    27361 gacttactgt tgaaaaatga aaaaaattcg gcagaccgtg tgtatttaag acagccaaaa
    27421 aatggagttt ggcatgaata cacctggtcg tctgtgatgc tgcaggccag aaaagtagct
    27481 ggttttttac accatctggg gttaaaaaaa ggcgatcatg tttccattat ctccaagaat
    27541 tgcgcggaat ggtttatcgc tgattttgga attcatattg ctggaatggt caatgttccg
    27601 ctatttgcta atcaaaatga agaaagtgcg cattatgttt tagagcatgg ggatgtgaaa
    27661 ctggtttttg ttggaaaatt agatgatcat cagcgggtca gacaatttat ccctaagagt
    27721 tatcctacaa ttggttttga ttaccataaa gatttgcaag ttgattacga atggtctgat
    27781 gttttgaaaa gtgaccctat gattaacctg gtggaaccag agccagatgc gttgtatacc
    27841 attatttatt catccgggac ttcgggggca cccaaagggg ctatgtatac caatcggtcc
    27901 atagccaatt acttgacagt tttccccaag gacttgctca gaatcagaga gttggaacat
    27961 tataaattaa tatcttatct tcccctggct catgtctatg aacgctctgc catccaatta
    28021 ggcagtgttg ctattaatgc atcggtatct tttgttgaaa gtctggagaa gtttgcagag
    28081 aacttaaggg aagtgagtcc tacctttttc actgcagttc cacgcatttg gggagtattt
    28141 aagcagaaaa ttgaacaaaa actgccacca gccaaattga attttttatt aaaaattcct
    28201 cttatatcca gtctgataaa aagaaagatc attcacagtt taggattaag cgagtgtact
    28261 aattgtttta gcggggcatc tcatctgcca atatccatta ttgatttttt tgaaaaattg
    28321 ggaatttata ttcaggaagg atatggtcaa tctgagaatt tggcttatgc taccttatcg
    28381 atgttaaatg agagaaaacg tggttacgtt ggtactccga ggctggaggt tgagataaaa
    28441 gtaggcgaag agaatgaatt gctaatgaag tgcccttgtc taatgtcagg ttattataaa
    28501 aatgaacaag cgactaaaaa tgcctttaca gaagagggtt ggttgcgaac aggagatgtt
    28561 gttgatgttg ataatgaaga cagagttaaa atattaggaa gactttctga gaactttaaa
    28621 aatcagacag gagagttcat tgccccttca ccgatagaaa aactgttttc agtgaaccct
    28681 atgctagaac aactgtgcct ggtaggtaga gagttaccta gtaacgtttt gttagttacc
    28741 ttgaatgaag gagtgcgttt gggtatgcca aaagaagaga tcactcacgc attgcaagaa
    28801 accttaaagc aagtcaattc caaattggtt aaatacgaaa aagtcagtca catccttgtg
    28861 ctgaaagatg cctggacaac tgaaaatgat aaattaacac ctacactgaa agtaaagaga
    28921 agggtagttg aaaaagatta tgccgagtta atccaaaagg cactgcagaa atctcagtca
    28981 atcgtttggg aatgaggaga cagtttattt aataaagaac tgatttcaaa tattcgttcc
    29041 tctgctgacg tgctgtctaa agtaaaacgc agacgttgtg gtccttccat ttgatagcgt
    29101 ttggcatgta cttgtattaa gctaatcagt gttccagggt caatcgatgg tttttcacta
    29161 aaatccaatt taccttgttg agcactggca ctgatttttt gaataccgag ctgtgcagct
    29221 tttaacttta attcagtaat caaaaagaga tgtttgactg gttgtgggag taaaccaaac
    29281 cggtctatca gttctatttg tagttctcta agttgttggg tagtttttgc attagcaatt
    29341 cttttataca tgatgagtct attgtgtata tcacctatgt aatcttcagg gataatcgcg
    29401 ctaattctca agtcaatttc aggtccttga tgcattggtg ctgacaattc aggagttttg
    29461 cctgccttta aatcgttaac ggcacggtca agcatttcca taaataaatt aaatcctatg
    29521 gcatgcatat taccactttg ttcttcacca aggagttcac ctgctccacg aatttccagg
    29581 tcgtgtgttg ccaaggtgaa tcctgctccc aaatcttcta aagacacaat ggcttcgaga
    29641 cgtttcactg catctggcgt taacagtttt tcattgggag ttaataagta agcgtaggct
    29701 tgatgatgag agcgtccaac acgaccacgt aattgatgca gctgggctaa tccaaattta
    29761 tcggcacgat cgataataat ggtattggcc gttggaatat cgatccctgt ttcaatgatg
    29821 gttgtgcaaa ccaggacatt aaatcggtga tgataaaaat cagacatgat gcgctctaat
    29881 tgcctttctc gcatttgccc atgtgcgctt tgaatttttg cttcgggcac taaagcttcc
    29941 agatcctggc aaactctttc tatagtttgt acattattgt gcaggtaaaa tacttgccct
    30001 ccccttaata tttcgcgcaa tatcgcttct cgtatggtga gatcattttt ttcttgccaa
    30061 aatgttttaa tcgccaaacg cttggcgggc ggtgttgcaa taagtgaaat atctctgatt
    30121 cccgccattg ccatgttcaa ggttctggga attggagtag cagtcatgga taaaatatca
    30181 acatgagtac gaagcgattt aatatgttcc ttttgtttca ctccaaatcg atgttcttca
    30241 tcaataatca gtaatcccag gtttttgaaa gaaatattat tttggaataa tttatgggtt
    30301 ccaatcacaa tatctacttt acctgattgc aatgcggcaa ggactgcttc ggactcttta
    30361 ttactgcgaa agcgggataa taattcaata ttaacaggaa actctgcaaa tctatcgcga
    30421 aatgactcaa aatgttgtcc tgcaagcaag gtagttggag caaggataca gacttgtttt
    30481 ccattttgta cagctacgaa tgcagcacgc atggcgactt cggttttgcc aaatcctaca
    30541 tcaccacaga ttaggcgatc catcggtctt ggtgattgca tgtccttaat aatttgttca
    30601 atagcctgaa gttgatctgg tgtttctgta aaggggaagc cactggcaaa ttttatatat
    30661 tcggattgat tgaaatcgta ttggtgccct ggctgggctt ctcttttagc atagagatcc
    30721 aataattcaa ttgccacatc atgaatcttc tcggccgctt tctttttttc cttttgccat
    30781 tgatcagaac ccagtttatg caaaggggcg tgttcactgt ccacaccagt ataccggctt
    30841 atcatatgca gagaagtgac tggcacatag attttgtcat cccctgcgta agcaaggacg
    30901 aggaactcac tgggggtacc attggagtcg atgtattgca aaccttgata gcgacctact
    30961 ccaaattgaa gatgtacaac aggggcgccc aatcgaagtt cggccatatc tcgtatgatt
    31021 aagtcagggt caactgattt ttgagtgctt cggcgttgag gtgtgctttg ttcaccaaat
    31081 aattgcgact caacgatgat cacaatttgt ttgtcggtta attcacagcc ataaatcaat
    31141 gggccagtag ttatattgat gggggattca tcgtaaatga aatctaacca tgagttctgt
    31201 gtttttggaa tgatatggct tgatttaagc agatccagta atacttcgcg acgccctgcg
    31261 ctttcaacaa caataagata gcgtcgagtt ggatcagagc aatactcact taattggcag
    31321 agtggctcaa tatttttcct gtcaatgggt agttgtggag ctttggcaat attgaaattt
    31381 agggctcctt ttttttcgga aggtttgtga tacaggcgta cttgttcata atggtttgct
    31441 ttagttaata actcggtggg attaataaag caattatctg ggggtaaaat agggcggctg
    31501 acatcatgac gccgttgttc atagcgttca tttaattctc gccagaatct ctcagcattc
    31561 tcctggattt cttcaattaa acaaattttg gcattggctg gcagataatc aaaaaaggta
    31621 acggttttat caaaaaacaa gggcagataa tattctatgc cagatggaaa ttgattgtta
    31681 ctaattgctt cgtatattgg gcaatggctg ggatttccag gaaaagactc tctgaaggct
    31741 cgtcggaaat gtaattggct ttgttcattt aatggaaatt cacgagcagg taacaaatgg
    31801 atttgattga ttttttctat tgtgcgttgt gtttccggat caaattctct gagactttca
    31861 atttcatcat caaatagctc aatacggaaa ggtaaaccac tacccatcgg atacacgtca
    31921 ataatagatc cacgtagtgc gtattcacca tgttcaagca ctttattaac acaatggtat
    31981 cctgcttgtt gcaactgatt cctgaacaag ttcagatcaa gcttttgccc ctctttaagc
    32041 attaaagcgt attgatttaa aaactctggc ggagaaagcc tgtgcattaa agtgctggct
    32101 gaagaaatga tgattccatt ggtaatttgt tgaactctgc ttaaggtgta gagtctttct
    32161 gaaataatat cttgatgagg agagaattga tcataaggca aggtttccca gtcgggaaaa
    32221 aataataact cctgggtatt ttggttcggg tttagaaaaa attgtagttc atcctgcaac
    32281 tgactggcgc ataggttgtc ttgcgtaata agtaatttga tcccgggtgt ttgttggcaa
    32341 tattcagcta aagcaagtgc cagactgcta ccatagagtt gcccccatat ttgtttattt
    32401 tttgtgggtt ggtaaagtag cgtttttgta ttcattggaa tttaatgatt aaaactgaaa
    32461 accgcttagt ataatgctaa attaggtatt ctgcaccata aagattttac tttagatgga
    32521 ttcagactcg accaattcta attgagttac ttcgtgttca tccaattcga tatcgaccga
    32581 ttcaattttt tggaatctgg aagtgatctt tttggctgat gtattgactt cattaacgtc
    32641 ctgatgtgcc aagtcaatgt gtttggataa tttatccatt ctcttttcaa agcgttgaaa
    32701 atcattggcc agggcttgaa gatgtttttg aatgatatgt acttgtttgc gagttgcatc
    32761 atctttaagt actgcccttg ctgtagtcag tacagccatt aatgtgctag gcgataccag
    32821 ccagactttt agcttttgag acatggcaat taaatcagga tagtttgcat gaatctcagc
    32881 aaatatagat tctgcaggaa taaacatcat ggcgccatct gttgtttcat tgggcacgat
    32941 atatttttca gcgatgtcct taatatgctt ttgaatgtct tggcgaaatt gttgttgtaa
    33001 tgacttacga tcagtggata aggcatcact gttgattaat cgttgataag tttccagggg
    33061 aaatttggcg tctatgacaa tatttccagt gggctcgggt aagaacaaaa cacagtcagc
    33121 ccgtttttga ttactgaggg tatattgcat ttgataactt gcgctgggaa gcatattgct
    33181 gatgagtgtt gataattgaa cttcaccaaa agcaccgcga gcttttttgt ccactaatac
    33241 gtcctgaagg ctgacaacat gactggagag ttcagtgatt ttcttttgtg cctcatcaat
    33301 gatggtcaat cgctttacca cgtcaataaa agtagacgaa gttttctcaa aaccttcggt
    33361 caatttatga ttaacctgct gagttagtgt gtttaaatga ttacgaattt cctcagttag
    33421 taattgtaag tgagaagtga gtgagctcgc atgatgtttg aagctatgag tcatttgctc
    33481 tctgacatcg gccatttgtc tttgtatggc atcactaatg agctgttgtg tagccagttg
    33541 tccttgagct atcttttcat taataaattg ttggctcgtt tgatatgatt ggcttaaatc
    33601 aatatgtaac tggtgaagtt gagagataag tgtttgttgt attctttcac caagttgttc
    33661 ggtcagtttt ttttgctgtg cttgttttat caaaacccag aaaagcaata agaattgaag
    33721 ggcaatagcc aacgctatgc cctcaaacaa agtgacagaa gaaaataatg ccattcatta
    33781 atccactaac tgactaagct tgacaggttg gttgtattga tacacagttt ttaactgccc
    33841 atagtctatc aagcgtaaat aacccaaata gaggttatcg tccgataatt gattgtgatt
    33901 gtcaaaggta aattgcaaaa catgcttacc gggttcaagc cactgcgctc tttgcgaggt
    33961 ctgaataggc ctggcttcac cctttccatt tttttggaac aacacacttt gtaaagcgta
    34021 tcggcttgca gttgcaacat ctacagtgac aacaaaagtc aaaggtttag aggacaattt
    34081 ttttacattc ataaggctgg ctgagggaat ggagtatgaa aaggctgtat ggccagatcg
    34141 tctaatgatt tcttgcccgt attctgtttg aacatcagtt tctaagtacc agttttcgcc
    34201 gcgatcattt aactcggaat ccaaagtggc tgttccttca aatacattgg attttaactt
    34261 ggtcagatta agcgaaatga cttgaccttt agggcctaca agtcgagcat caacgtcatt
    34321 gacgtcatac tcagtgatat cattgtgaag cgatatggtg gcttttagct tatcgccata
    34381 ctggtaatgg agggaatcgg tttcaacctc caggtaagtg atggaaaatt tatcaagaac
    34441 actgatcata taagcatcgg catatttgtt agtaatgtcc ttgcttttta gtataaattt
    34501 tccatacccc agttcaggtt ttatttgtaa catagcctgg tgttttgtaa tggctaaagg
    34561 attattacca acctcgtcat cctgattata gagagaagag gcttctttca ggggcaagag
    34621 ttggtttttt ggcgttttga taagtaattc cgggatggat ttattctgca aaggggtaat
    34681 gcgaacgaca gcgccaggag ccaatgttga tataataact ccgcgttgta attgttccag
    34741 agaaatacgt tctttgtaac cataacttct acgaacatta ctgatgttgt tgtttaatgg
    34801 ttcattggta atttcccatt tatcgtgcag attttctcgt gataattggc tgcaattctc
    34861 acattcataa gatttggttg gttgtcttgg taatgtaaac gcatgagctt gggcaataca
    34921 aaatgcggat atccatataa ggtgggcagt tttcatttta caactccttc attaactagg
    34981 gcactgataa gtatttgatc caaaccaaaa caagaatgtc gactttgatt atggcttaaa
    35041 ggcaaattgt aatcagtttt gtctatatcg tagaaccata cttctcctgc ttgagcttgc
    35101 gactcacaat ccagaaagcc atcggcacac tgattaagat agagtttggc aatttttagc
    35161 ccgctattta gcatgtatcc attgcaatag ctggatgttg aaaatggtga agcatagaca
    35221 tcagtaccca ctatgacttt aaatggaatg gacagagagg gtcggccttt gcttcccaat
    35281 agcaactctt cgttatagat cagcatgtcg cctacgcgtt gctgtttgac tgcatctgaa
    35341 agatagccga gtagccaggc caaactcgtt tcaaatatgc cgccatttaa tacttcatca
    35401 gcaagaggag tccctccgct ggaaggtgca atggcaatca cttgactgat tttgcgtttg
    35461 agagtcatat atcggccatc ataagttgga tttgagagaa tccaacgcat gatgttggca
    35521 ccatcagagt gggtgtagac tgtgagagag ctcacatttt tatcgttaat aaattccaga
    35581 agttgattgg caacacaatt ggctgcatct tcatgccaca tatattggct aaagtcacaa
    35641 tgaaccacgt agtgattttc aggttttgcc agggtttggg agatactttg tatgaaatca
    35701 attttccaat accctccata ggcatcttct ctgtgatctc gagtaccatg gacaaaagca
    35761 atgccttgat tggaattagt gtcggaatga gaatggataa gggttgaaaa caataaaaca
    35821 ataagagtgg ataaaatgcg cagaagttcc attttcaaaa tatctccttc aatacagaga
    35881 cgtatctata tcatggttct ggcaaaagat tcaagagatg ggatcgatgc gttttagtgg
    35941 atttgcgccg attacttggt tccgaataaa gaggtttcca aaaaaagatt ttgccttgaa
    36001 tgattaaacc actcatattt gaaagaagta aaaaaatgat aaactgttgt ttttattttg
    36061 ggaatcttca ttaatgaatg atcaaggaat taaggaatcg atagaaacgc ttaaagagca
    36121 aataagaaaa tacgattatc actattatgt tttagatgaa cctttggttc ccgacgcgga
    36181 atatgatcga tgtttcaagg cattgcaaca gtatgaagag caatatccgc aatttttatc
    36241 gccggattct cctacacaga gagtgagcgg tactccttca gatgctttta tgccggtagc
    36301 ccataagcaa cccatgttgt ctttgtccaa tgtgtttact acagatgaat taaaagcatt
    36361 cattaagcga gcaattgaga aactggatga accaaatcaa caactggtat ttgcttgcga
    36421 accaaagctt gatgggttgg ctgttaacat gacttatgag ggcgggatct tgactcatgc
    36481 cgcaactcgt ggcgatggtg ctgtaggtga aaacatcaca gcaaatatta agactattgc
    36541 ttcagttcca ttaaggctaa gggttagtaa ccctccaaaa ttgatcgaag tgcggggtga
    36601 ggtctatatc ccgaaagccg attttgaagc atacaacgca agggctagag aactcggtga
    36661 aaaaactttt gctaatccgc gaaatgctgc tgcaggtagt ttaaggcaat taaatcctga
    36721 aatttctgcc aatcgcccac ttgctattta ttgttatggt ataggggctt tcgaggatta
    36781 taagttacct aacagtcatt tggagcaatt gaatttatta aaagaatttg gatttagagt
    36841 gtctccagaa acgaggaggg cggttggaat agaaggctgt ttagattatt accagtatat
    36901 gttagcgaaa cgaaatcaat tgccatttga aatcgatggg gttgtttata agattgacag
    36961 tatctccttg caacagcaat taggttatgt ttctcgtgcc ccaagatttg cttgtgccca
    37021 taaatttccc gctacagaag aaatgacccg tctgatagcc gtggatttcc aggtaggtag
    37081 aacaggtgct gtgacgccgg ttgcacgttt ggagccagtt agtgttggtg gtgttacagt
    37141 aagtaatgcg actttgcata attttgatga aattacccga aaagacattc gtattggtga
    37201 tacggttatt attcgtcgtg ccggtgatgt gatccctgaa gtggtttctg tgattttgga
    37261 aaagcgtccc gctaatgcca gaatgattga gcttcctaaa aattgccctg tttgtggttc
    37321 tgaagtcgta agggaggcgg atgaagcaat tgctcgttgt gtcggtggtt tatattgcaa
    37381 agcacaatta aaaaggatga tgtggcattt tgcttctcga aaagcaatgt atattgaagg
    37441 acttggcagt gttttaattg accagttagt tgatgagggt attgtccatc atttggcgga
    37501 tctttatgaa ctcgatttgc agactttagc tagcctgcca aggatggggg agaaatctgc
    37561 aaaaaaccta ttatccgctt tggaaaaaag taaaaaaaca actttcaatc gctttcttta
    37621 tgctttgggg atcagagaaa tcggtgaagt cggcgcaagg gttttagcgg agcactactg
    37681 cgatgtagag ggcttgaaag cggcaacgat tgaggaatta atgactctga atgacatagg
    37741 tccagtagcg gcttctcatg tagtccattt ctttgcccaa gcgcataatc ttgaagtgat
    37801 tgaccgtctt ctcaagttgg gtattcattg gcctaagtcc gaaaaaatac aggttaatca
    37861 gcaaaatcca ttttttggta aaacagtagt tttaactggg actctgagta ccatggggag
    37921 ggaagaggca aaggcaaaat tattagcctt gggcgcaaaa gtgagtggaa gtgtatcttc
    37981 caaaacggat tatgtagtag caggaagtga agccggttca aaactgatta aagcgacaga
    38041 actgggagta gtgattatag aggaagacga gtttttaaaa tgggttaatt catgagtagc
    38101 tagatctttg ttatttggtt tatgatgggt atcaatgaat cgggtgaagt aggtatttaa
    38161 gcctgtaata atgactgatt ggcacgaggg aagtaagtgc ggcgatattt gttaattcaa
    38221 ttcatgttat ttttaatgcc ttgctgtgtc aatttggctt atggcagttc ttgggtattc
    38281 aataatcctt atccagagag cgaggccaat gaaaatattt attattcttc ttttaatgaa
    38341 cagccaaaaa ctctggatcc agcacgctcc tattcttcca atgaatatct tttcatagcc
    38401 cagatctatg agccactgct ggaatatgat tactataaaa gaccctatga attaatccca
    38461 ttaacagcta cccgcttacc acagattcga tatttggatg cccagggaaa tcctgttccg
    38521 gcaggagata acacaaagcc agcatacagt gtatatacca ttcagataaa aaaaggaatt
    38581 ttatatcaac ctcatcctgc tttggctcag aagaaagatg gctcttatcg atatcatcac
    38641 ttaccagaag attatttgga agagcatgat attaacaagt tgtcagattt taaatacacc
    38701 ggtacaaggg aactgatcat tgatgattac atttatcaaa ttaaaagatt ggcaaaccct
    38761 gctgtgagtt ctccaatata tggattgatg agctattata ttctgggctt caaggagtat
    38821 ggagatagtc ttcctaaagg attcgccaat aaaaattctt ttattgattt acgtaagtat
    38881 cctttgcagg gggttaaaaa attagatgat tacacctttg agattacctt aaaggggcaa
    38941 tatacacaat ttctgttttg gttagccatg cccttttttg ctccggttcc ttgggaagcg
    39001 gacttatttt attcccaaaa agatatggat gataagaact tatcttttgg ctggtatcct
    39061 ataggtacag ggccttttat gctgactgaa aataatccca atagaagcat gatattggag
    39121 aaaaatccta atttcagaga agtgtatttt cctgtcaatg gttcagatgc cgatagacgg
    39181 gcaggttatt taaccaatca gggaaggcgc ttgcccttaa ttgataaagc cgtttatacc
    39241 cttgaaaagg aatccatacc tcgatggaat aaattcttgc aaggctacta tgatatgtcg
    39301 gggataagtg ctgacagttt tgatcaagct attcatatta atcgctttgg agaacctatc
    39361 cttagccagg aaatgaaaga taaaaaaata tatttaagac aaaccctgga accgtctata
    39421 tattatactg gctttaatat gcttgacagc gttgttgggg gcaatagcaa aagagcacga
    39481 aaattacgcc aggcaatttc tattgcggtg aattttgatg aaaatattgc catatttttt
    39541 aatggccgag gtattccggc gcaaggccct attccacctg gtatctttgg gtacaaggaa
    39601 ggaaaagagg gtattaatcc ctatgtttat caatgggtga ataatgccag gaaacgaaaa
    39661 tccttaaagg atgcgcagaa gttaatggtt gaggcaggat atccgggagg gatagatccg
    39721 aaaaccggga aacacctgat tcttcattat gatgtcatga cgacaggtgg acctgatgat
    39781 aaagcaaggc ttgactggat gcgaaagcaa tttgcaaata tagggataga tttgaatatt
    39841 agggcgactc tttacaatcg tttccaggaa aagatgcgga taggtcatgc tcaaatattt
    39901 agttggggct ggaatgccga ttatcctgat cccgaaaact ttttattttt gctttatggg
    39961 aaaaatggaa aagtgaagta tggtggtgaa aatgcatcaa attatgaaaa tcctgaattt
    40021 gatagattgt ttaatttgat gaaaaatcgc cctaacgatc tacaaagaca aaaactgatt
    40081 gataagatat tagcgattgt ccggtatgat gctccatggg tatggggcat gcattctgaa
    40141 gactttattt tatcgcaaga ttgggtatct aatataaaat ccaatacgat ttctctcaat
    40201 actctaaaat atgtttctgt taatgtacct gaacgtaacc aactgcgaag atcctggaat
    40261 caaccggtat tttggccttt aggtgtgctg tttggcctat tgattgcttt ggtaatccct
    40321 ttattgatta cttatttaaa gaaagaaaaa cagccggcag aaagagtaaa attaaaatga
    40381 ttaaatacat actccgcaga attttatatg cgattccaat tttgtttggt attaatctta
    40441 ttacttttgc attgtttttc atggttaatt ctcccgatga tatggcacgt atgcacttgg
    40501 gtaataaaca tgtagagcaa aaagccattg aagattggaa agcggttcat ggctatgatt
    40561 taccgttatt ttataatgaa aagcagactg gatttaatca attcagacag actttgtttt
    40621 atcaaaaatc gctgatgctg tttacattta attttggtgt ctcggatgct ggtagagata
    40681 ttagccacga tatatcctac agaatgtggc caagccttgt cattgccctg ccaacattat
    40741 tatttgctgt tttggtgaat atcatttttg ctatggctat ggcatttttc cgttcgactt
    40801 atcttgattt atccggggta gttatttgta ttatcctaat gtccatttcc agtttgtttt
    40861 acatcattgg cgggcaatat ctgtttggaa aaatattaaa atggttccct atttccggct
    40921 acgatgatgg cctcgatgct ttaaaattta ctattttacc agtcctggta gctgttctgg
    40981 gacaattggg cgcgggagcg cgttggtatc gaaccttggt tttggaagaa attaataaag
    41041 attatgtaaa aaccgcaaga gcaaaggggc tctctgagca aaggatttta tttaagcatg
    41101 tattaaaaaa tgccatgtta cccattttga ccggaattgt tgtaatcatt cctactttgt
    41161 ttatgggaag tttggtttta gagtcttttt ttggtgtgcc aggcttgggt agctatatta
    41221 ttgatgcgat acaacagcaa gattttgcta ttgtacgagc tatggtattt ctggggtcag
    41281 ttctttacat cataggcttg gttctgactg atttttctta catcctagtt gatccccgag
    41341 tgcgattatc ctgataacca aggagtatgt ggcaaaaatg acatttgaat tgctttggac
    41401 tgacaaatgt tttcttactt tactggcggt ggttttattt accgttctgc tgagccttag
    41461 aaaacaacac atcaaattgt catttgcaat cattttacaa aggccgttag caatcagtgc
    41521 tggaattgtg ttgttgctgt ttttagccat cggtgttttg gactccattc atattaaatt
    41581 catcaatgac aaaacagaaa aaactcagtc tctattggat agagttcttg cacctatagg
    41641 aaatacctat gagaaaacct attcagctcc cttggcttta aatcaattta tgagtgaaac
    41701 aaagttggtt aatggagtta caactcaggt ttatcctcgc ttaaaatatc caccagcttc
    41761 tattaagaat gaaaaggaca aatccctcat catcaaatct gtgattatga aaggtataac
    41821 atacagtgct ttagtcattt tgggtggatg cttattactc gctttagtaa agtttagtct
    41881 tggatataaa aatactttca ttttaaattc tactcaaatc agcgctatag cgactgtagc
    41941 tgtacttact tttgcggtcg tcattggtta cggactatca agatcctttc atgtcttggg
    42001 cacagggcaa atcggacaag atatttttta tttcagtttg aaaagtgtgc gaacgggtct
    42061 aatcattggt tttttaacaa ccctcttcat gttgccactg gctttatttc tggggattgc
    42121 ggcaggatat tttggtgggc ttgtggatga tattatccag tatgtttata ctactttaag
    42181 ctctattccg ggtgttttgc taattacggc ttccgtattg tcattgcaaa cttatattgc
    42241 aaaccacccg gagcaattta ctactttggc caagagtgcc gatgcacgtt tattagctct
    42301 ttgcttgata ctgggtgtga ccagttggac aagcctgtgc cgacttttgc gagcagaaac
    42361 tttaaaattg cgagaaatag attatgtcct ggccgcacgt gccttaggca gtagttggtt
    42421 taccatcgta cgcaaacatt tggtgcccaa tgtcatgcat attgttatca tcaccctggt
    42481 attggatttc agttttttag tcttggcaga agccgtattg acctatgtag gggtaggggt
    42541 ttcacccatg actattagtt ggggaaatat gattaatggg gcgcgccttg aattggcacg
    42601 tgaccctgtt gtatggtggc ctatgtttgc agcctttctg ttaatgtttt tattggtttt
    42661 ggcaatgaac ttgtttgcag acgcagtacg ggatgctttt gacccacatc aatctcattt
    42721 atagtcaata tctgttagtt tcctatttcc gggatttagg ttaaagtttc gaaattatcg
    42781 tgaatgggtt aattctctat gcgacaacgt tttgttcatt taagagtaca tacggaattt
    42841 tcattagttg atggtttggt acaaattaaa ccattgatga aagctttacc agccagaggt
    42901 atgagcacag ttgctgtaac ggatttttgt aatttatttg ctgctgtcaa agtatttaaa
    42961 agtgcaacag aggcgggaat caaacccatt attggtagtg atttaccatg ccatgatttg
    43021 gaaaccccgg ataacatttt ttctttggtt ttattatgtc aagacacagc aggctataaa
    43081 aatttaacat gtctggtatc caaagcatat caggaagggc aatatcatgg ccaacccagg
    43141 gtgcaattac aatggattcc agaattttca cagggattaa tcgcattatc cggaggaaag
    43201 ttcggtgaca ttgggcaagc attgctggca aatgatgaca ctttggcaag aaaacgtgct
    43261 ttgcattgga tgaatttatt tccgaatcgc ttttatctgg aaattcagcg aaccggaaga
    43321 gctgatgagt tgcattataa tgagaagctg atcgcgttag cggacgaatt gcaattacct
    43381 ttggttgcaa ccaacgatgt taggttcatc gatgaggatg attttgatgc tcatgaagct
    43441 cgtgtatgca ttcacgaagg gtacacgttg gctgatccca gaagagttca acaatacagc
    43501 tcccaacagt atctgcgctc agcagatgaa atggagctct tattttcgga tcttcccagt
    43561 gcattacaaa acacagtcga aattagtaaa cgctgtaatg tgaaactcga gctgggccat
    43621 aactatctac ctaatttccc tataccggat ggaacaacag tagaggcgta tttatcacat
    43681 ctttctgaaa ttggcctgga ggaacgttta aagcaattat tcagagacaa gtctgagggg
    43741 gagctgcttg ctgccagagg ggagtacgac aaacgcctta aaattgaact ggatgttatt
    43801 aataacatgg gctttgctgg ttacttcctg attgttgctg attttattca atgggctaaa
    43861 caaaatgggg ttccagtagg gccaggtcgt ggttctggtg ctggttctct ggttgcttat
    43921 gccttaaaaa taacagatct ggatccttta gagtatgagc tgctttttga acgattctta
    43981 aaccccgaac gtgtttctat gcctgacttt gatattgatt tttgcatgga aggccgtgat
    44041 cgggtcattg aatatgtagc agagaaatat ggccggcaga gcgtttccca aattattact
    44101 tttggcacta tggccgcaaa ggctgtggtg agagatgttg ggcgtgtttt aggtcatcct
    44161 tatggttttg tggataagct tgcgaaatta attccctttg agttaggaat aactttaagt
    44221 aaagcgatgg agcaggaaga agagcttcgc aggcgatatg aagaagaaga ggaagtaaaa
    44281 gaactatttg atcttgccct gaaattggag ggaattactc gtaatgcagg caagcatgct
    44341 gggggagtag ttattgcacc ttctcaactg actgatttca cggcgatata ttgtgaagaa
    44401 ggatcaacac aaatagttag ccaatttgat aaagacgatg ttgaggcggt tgggttagtc
    44461 aagtttgatt ttttaggatt aagaacacta accataatcg attgggctct ggccacagtg
    44521 aataagcaat gcgaaaatgc cgggttgtca ccaattgata ttagtcaaat tccaacgagt
    44581 gatgactcta cttttgagtt attaaaagca tgtaaaacta ctgctgtatt tcagctggaa
    44641 tctcgaggca tgaaggagct gattagccgg cttcaacccg attgttttga agacatcatt
    44701 gctttggttg ctttattcag accaggtcct ctacagtcag ggatggtgga tgactttatc
    44761 gacaggaagc atggtagggc tcgagttgat tacccccatc cagacttgga accaattctt
    44821 aaacccactt atggggttat cctctatcaa gaacaagtaa tgcaaatcgc tcaggttctt
    44881 gccaattaca cgctcggtgc tgctgacttg ctaagacgag ccatggggaa gaaaaaaccg
    44941 gaagaaatgg caaagcaaag ggaaattttt acccagggag ctgttgcgag aggagtagat
    45001 gagcaggtag caacccatat ttttgattta atggagaagt tcgccggata tggattcaat
    45061 aaatcacatt ctgccgcgta cgctttagtt gcttatcaaa ccgcctggct aaaagcgcat
    45121 tatcctgcag cttttatggc tgcggtgatg tcctctgata tggataacac ggataaggtt
    45181 gtaactttta ttgatgaatg cgcgcacatg aaattacaag tgttgcctcc ttctatcaat
    45241 aactccatgt atcattttac cgtgtccgat gaaaagacga ttatttatgg ccttggggca
    45301 ataaagggag ttggcgaatc tgcgattgag tgtattattc aggagagaaa ccagaatgga
    45361 ccttttttgg atttgtttgg tttttgtcaa agattggatt tacgcaaagt caacagaaga
    45421 gtgattgaag ctttaatcaa gagtggtgct tttgatgttt ggaaagtgga aagggctgta
    45481 ttaacagcgt ccttggaaaa agcattgaaa gttgctgaaa aggaacacca aaatcaatca
    45541 agtggacaat ttgatctatt ctcgctttta gaggacaaag cggatgaaca aaattatgtg
    45601 gaaagtaaat cctggtctga agctcaaaga ttagaaggcg aaagagaggt attaggtttt
    45661 tacctcactg gccatcctgc tgaccaatac agacgtgaat tgggagattt tattgttcct
    45721 gtaggccaat taaatccgtc tttacataaa aaagcgatta tttgcgctca agttgtagca
    45781 attagaaaaa ttatcaccaa acgtggcaag aaattagtga ttattggaat ggatgatgcg
    45841 actgccagga ttgatgccgt agtttttggt gagatttttg aacaatattc tgatccatta
    45901 gggacgggta atatgcttgt aattgagggc gaggttgcaa ttgatgatta caacggtggt
    45961 gttaaaatga ccgccaatca gcttttaagt atcgcttcag ctcgaacaaa attcgctcgt
    46021 tgtctggagt tgcgtttaaa tcaaaacaat gccgctgtat tgccttctat ccaatcctta
    46081 ctgaagactt attcaggacg gtgtgctgtt caatttgttt atgctaatgg tgttgctaaa
    46141 gctaatttaa atcttggcca acaatggtgt gtgaccccta gcgatgaatt aattggacta
    46201 ttgagcaatc ttttagggga agagcaagta ataatgcgtt attagaaata ctgctaatga
    46261 tagcgcctgt ggagttgagc agatagcacg gctcaactcg cattgcagaa tgatagttgt
    46321 ctcatctaca tcaaacagtt gctagcaatg tggaagagga agagtcttct tcgctaactt
    46381 tttcttcatg tagcttcaat tgagctttta attcattaat taactctagt ggaagttgaa
    46441 ctccgccgtc aggaatagtg acttcttttc cattgataag aagttttcct tcttcagtaa
    46501 tttcaactgt ttgctgtcct tcttcagttt ttaaatcaga gcttggagtg tattcaatca
    46561 ctatgccatt catgatggat ttgccatccg caatacgaat acccgtcatt tgagaggtat
    46621 taggatagtt gctgtagtaa ttcataatag tggcgctggc tttccatatt aatctgtagg
    46681 cataatccaa aggtaacccg atgcccattc ctacgccttg cccgagaata cccatggttg
    46741 ttcctaaaac atgagccaaa ctaatactga aaaatgccgt gactaattta ctcgctataa
    46801 cctgagagaa caacgccacg cccataggtc ctgttgtacc aagcattagc aggctgcttc
    46861 ccaccatggt agtaatttgc agtttggtcg acatcgcttt ggaggattgg ctaataacat
    46921 cccccccaag ataaccaatc gttgctcctg tataagaaaa agcagctccg aataacggtt
    46981 gagtaatgga tgtgacggga tagttcgagt acggattggt agataccttt ttaacactta
    47041 cactactgac aaacttggaa aaatcactcc actcctgctc ggtaaatggc ttcatattgg
    47101 ctttgcgcaa ggcagcgttg gcatcgctaa atgcggataa agaggaattg accactctat
    47161 tggcagctaa aaagcagatt tccttgtctt catcatccag ataattggta tcagtaatgc
    47221 ctaaatcctt attaatcaat cgatataatg cggagttatt tttgggagat gaaatttggg
    47281 attgaacata tagacaggct gcgatgacga ctctggaagc agttaaattc gcttcccatt
    47341 gttcaggggt tgtgatttct tcctctttcg ccagatttgc tctaaggaca acaatcattt
    47401 tttttataaa tttggtttgt ttatctctgg ctatgacatc agtgcctcca aaaagagtag
    47461 gtactccgat taaagatggg ggttgatcca aatctacttt tctttctgat tgatagtcat
    47521 cgcgtaaaaa gttaaggctg gttagagctc ctgctggcag tatatagcgc ataataacct
    47581 catatccatt gaaaatttat ttgtgtgttt tttatcttac cgattagctg gttaaatgca
    47641 atctgatttg gttaattttt actaaattgt tgcaatcaga taaggctaca gattttattt
    47701 atgatttaac tctttgaaat gatgattttt ttgcgattat actattttta gccttgagtt
    47761 agatatgtta ctcttatagg gtgtaattaa tttaaaatta aaacaaatcc atgtcctgta
    47821 gcctgattga ggcggtcatt aatactttga ttagaaatga cagaactgtt ttagctgttt
    47881 ttgagaaaag ggtagatttg tatgatggaa ttagcaaata aaatcatcag ggtaagttac
    47941 atggataagt tataccaata tcttttactt cttcgtctat cgcactggag taaagcaatt
    48001 tttgttatgc tcggtttcat ttatactcct gttccagggt atttgtttcc ggcattaatg
    48061 gcttcattat ctttttgtct tattgcaagt gccgtttata tttataatga tattcaggat
    48121 agaacggaag acagtctgca tccctttaaa tgcaacagac ctatagccag cgagcaggtc
    48181 actttatttg atgcgatcat tgtaatgctt ttattgatgg ttgctggaat cgcgctgggt
    48241 tggcttattt caaaaaaact ggtggtcatt ttaattattt atttgataat caatcttgct
    48301 tataaccatg ttttaaaatt gatcccggtt tttgatgtcg cttgtattgc cctgggattt
    48361 atgctcagag ttttggccgg aacaatagga attgatttgg ctatatcagc ctggttaact
    48421 gttgcagcaa ctttgcttag tttatttatc gccctaaata aaaggcaact ggaaatgcag
    48481 ttaggattga aaaattcaac ccgtaaggtt ctacgccgat ataatccagc ctttctgcaa
    48541 agacttatcg tcattacagg aatagcctgc tttattactt atctgtttta cacggtttat
    48601 gccagagcag aatcgttttt tttcatttta actttacctt ttgctgctat tgcactttgg
    48661 cgttttgcct ggcttgccac tcaggatgta gagaatgatg atccggtcaa tattttttta
    48721 agtgatcgat tatctcgaat taatttatgg tgcttcgcta tcctgacctt tatggcattg
    48781 aattgatgag tgctaagtgg cgctggtatg acagcctgtt tatcgttttg tttgcttatt
    48841 tgctagtttt acaaatccag gccatatggc cgttcactat agatgacatg tatatttctt
    48901 tgcggtatgc caaaaactgg gctgcaggaa atggcttgtt atggaattta aacgctgagc
    48961 cggttgaagg ctattccaat ttcagctttg ttgctttggg ggcaatatca attattctgc
    49021 acattaatcc ggttcttacc ctaaaaatcg ccgggtttgc tggattactt gccacgtgtc
    49081 tttttatcta tcttgtcagc cgcttttggt ttaccaaacg agagtctttt atcccctgca
    49141 tttggctttt actatacaaa ggacaagtta tttggtcagt tagcggcctt gaaacgaccg
    49201 tttaccaggc actgattagc ggttcagtct attttgcttt tcgcgggtta ggatatcatt
    49261 tttatcccgc tcctcgaggt gaatttaaga caagtggttt tgtcttgtct gggttgtatc
    49321 tttcattcgc aggaatgacc aggcctgaaa caccagccct gatgcttctt ttttttcttt
    49381 taatcagttg ggactggaat aaattatcag gcaagaagcc atggagaggg gcggtatctt
    49441 ttgcggtgac cataggtttg gtgtttctgc cttattttct ttggcgttgg agttattacg
    49501 ggttattgtt tcctaactct gtatattgta aaggttttaa tggcatatcc tattcattgg
    49561 atttgaatta tctcaaatta atttggccat ttgctattct ggctttgcca gcctgttttt
    49621 atgctcagga cagaaggcat tattttttat ggtctcccgg tattatttat ttattaatgc
    49681 ttgtgaatgc cgatccagtt gtcgcttttg ataatcgatt gttcttgcca gcgtttgctc
    49741 ttatgttacc cctgaccttg aatggtatca gttatctttt attaagctat ttcaaaaaac
    49801 aagataatta ctttagagcg gcattgtatg tgagttcatt ttttctggcg ctactattta
    49861 tacccaaaat gtcccttgat gcttatcgct actttagtca aaatccggta catggagagc
    49921 aattacggaa aagtgttgtc cagtggttaa atactcatgc caagaatcaa gatagagtgg
    49981 tgttggcaga ttctggtttg attccgtttt taagcggatt aacttttatt gattcttact
    50041 gcctgaataa taaagaaatg gcacaatttc ctgccgtgaa gagatatgaa ttattttgcc
    50101 agcagatttt agaagaaaaa ccacagataa ttattttgac tgcgcttatt gataaaggaa
    50161 agatcattta tactcccggt gattcttgtt tgaaaccatt tcttgatgtt cataaagaat
    50221 acaagttggg tggtgttttt tcttttggca gtgctgagtc acaatatagg tatgaattgt
    50281 ttgctaattt ttgatgcggg aaactgtaac atagtgtacc atctttcctt tataaaggat
    50341 tacagactgt ggtttttata atgcaaaaaa taatcatatt gacaaaaaaa atcattgcaa
    50401 aactcccggt aattttgttt gatgtggctg caataccagt tgcctggtat gccgcttact
    50461 ggttgcgcta taacatgcag ccatatcccg gcagcctgac atccacccat tcttttattg
    50521 ctttagcttt attatcaatt gttcagatta gttgttacta ttattttaaa atttacagag
    50581 gattatggcg tttctcttct ctgaatgatg ttatcaggat actgaaggct actatcacag
    50641 caatggtttt agtaatccct gttttttact tgacttcaat tcttcagcac ctgcccagat
    50701 cagtttttcc tttgtattgc attatcctgg ccaccatcct ttgcgggggt aggttggtga
    50761 tccgtctgca ttgggataag cctgacagag gaaataggga attggaaacg aaaagagttt
    50821 tggtggttgg tgccgggcag gctggtgaag gcttggtgcg tgatttgaaa agaagcagtt
    50881 attatcggcc aataggcttt gtagatgaca ataaaagcaa gagaggatta gaagttcatg
    50941 gagtgcgggt tctgggaacg accaaccaaa ttaccgaatt agtcaatcaa tacgacgtgg
    51001 atttgatttt cattgccata ccttctgcca aatcggcaac catgcgaaga attgtaacgc
    51061 tttgtgaaca aagccatgtg ccttttagta ctttacccag tatttcagct ctggctgcgg
    51121 gtcgagttga agtgaatgcg ctgaggcctg tgaatattga ggatttattg ggtcgggatc
    51181 aagtgacgct cgaatgggaa aaaattgctc gttcaatcgc aggaagacgt atattgatta
    51241 caggcggtgg tggctcaatc ggttcagaat tgtgccgtca ggttatggcc ttagagcctg
    51301 cgagcatagc tattgttgag aatagtgagt ttaacctgta tcgcattgaa caggagttgt
    51361 taaagagttt tccaggtatc cccgttgaat tgagtttaat cagtgtgaca gacgaaattg
    51421 cgattaatca tttatttcgt cggtttcagc ctgaaattgt atttcatgcg gctgcataca
    51481 aacatgttcc catgcttcag catcaagtac gggttgctgt gtttaataat gtcataggta
    51541 ctcaagttgt tgccaaagcc agtgtgacat ttggcgctga gaaatttatt ttaatttcca
    51601 ctgataaagc agtgaatcca acgaatataa tgggaactac caagagagtc gctgaaattt
    51661 attgtcagaa tttaaatgca cgagttaaaa cccagtttat tacggttcgt tttggaaatg
    51721 tcttgggttc agctggtagt gtggtgcctt tgttccaaaa acagttgcag gaaggtggtc
    51781 ccctgactgt gacccatccc gatatgcaaa gatattttat gactataccg gaagcatgcc
    51841 agttgatttt gcaagcgatg gtgaatgggg agggcggtga aatttttgtt ctggatatgg
    51901 gagagccggt taaaatcagt tatcttgcag aacaaatgat tcgcttggca ggcaaagaac
    51961 caggaagaga catcatgatt gaatatacag ggttgcgtcc tggcgaaaaa atgtatgaag
    52021 agttgtttca tgagacagaa cagttaactt caacaaaaca tgaaaaatta ttcaaagcaa
    52081 ggtttcgtga actggattgg aatgatttga cacaaaccat gcgaatgttg caggcagctt
    52141 gtattgagca tcaggatgaa gaattactcg ttctgttaaa aagtctggtg cctgagtttc
    52201 aattggaacc tatggttgaa gcttaagcaa tagacctaat ctggtaaatc aagatgctgt
    52261 tatatcaatt acttatgttg attttgaaac agaattttct cataatttga tggtaaaatg
    52321 aaaaacagca aaagaatttc ttagcttctt acatcttcct gggcccttgt tttagaacct
    52381 gggaccgtga tttggctctt cctgttcgtc cagtttctcc gcatgtatta aatttgtggc
    52441 tgcatcacga aatgccagct tcgcctgatt aaaggcttgt atgcaacgct cagactcagg
    52501 gagacactta gccacttcta tagttttata ttctgctccc tcgtggatcg tatgttgaaa
    52561 atcttcccat gccgaaccag attgtccaga agtaagagaa ccaagttctt tatttgtagc
    52621 agctacaata ctctgctttg cgggttccaa ttttttttcc aatacatcaa gcgcaaaatc
    52681 aacgtagtca tctatcgtta aacccatttc gtcctcgaag cgctcatcga attcatcttg
    52741 tgtttcgtta ggccgaattt ttaccccgct tgattttatt atagcttgct tcatgctttc
    52801 atctattgga gtttcactca gaaatgccct tgctttgtcg tgtacggcca tcattgccat
    52861 acaatactct ctccttgcgt taaggtattc ttcctcaagt tgttcactac ttgacccggt
    52921 catgatatct gctccactca tatacctata tttaaaaggt agcattcgat ttataattat
    52981 tcaaaatgac aataacaata ccaattgagc tgtggtgaat actaagtcaa tatccctaaa
    53041 tttttattaa acatttagtc ttgaattgag cagggatttt aatttcactt cgatctgctc
    53101 tctcgcccca tgagcttagg tagtatggtg atgcatttcg cggaagattt ctggggggag
    53161 gggtgggtaa aatctgtgct aaccaccaga cccattagca ggtatatgat gcataatctc
    53221 attataaaga gcgctccgga ttacaggaaa gctgtaatcc gggagaacat ctctgataac
    53281 aggtaaattc ttatatcaag tattttttta ttgtggactt aatcgttatt aaaggcttag
    53341 gttctcttcc tgtacttttt gcaagcttcc caaagtatct tcaagtttga gtttaatttt
    53401 agtgatttca tcagatacct gatccacaca ggttctggtt ggagccaaaa actgcggtgg
    53461 cgtgttgaaa cctattaaac taatcgccca gcgggcaatt gctgtcaaaa tatcggctat
    53521 ttttgtcaat accggacgat tgtcagctaa cttttcagct gcttctgaac cgtattgact
    53581 acattgatta aaaaatttct ctacatcctg tggagtaatg tcttcaatca gtttatcacc
    53641 cagcaattgt ttctttgcat tttttatatt tgcataaaat tcttgggcta tcgccagagt
    53701 ttcattatcc tgagaatggg agacgtcggt cataagtgtc tcaattccct tgaaagttgg
    53761 aatttcaaag cccataaaag ttaataactc aactctttca ggaattttat ccaggattgc
    53821 tttggttttg gtttgaatat attcattaat ttgttcgaga ttcatcttgg caaaaagatg
    53881 gatctgttta tccattattc tcccaagaag ctgatacaat tctgtcgcag cttgtagtcg
    53941 aattttctca ttaggatcaa cttgtttaaa aacttctgtc ttaataattt gataaataaa
    54001 ttctttacta tgtattagct ctgcaatggc cttggccttt ttgtccgata tagattcaac
    54061 aatgtaattt tgggaatcag gaaatgccat gcttaataat tgggcatcac attgttgagc
    54121 ggtatattgc aaagcatctt gtggtgatat tttcagagct tgagagtaaa tctcttcaat
    54181 ttccctgata tccaggccaa atttacctaa tttgactttg cctaaagcat ttaatctttc
    54241 gttttcctca acagttctgt cgatgctatc aagtaatgtt tttgttaata catcggaact
    54301 ggatagcgcc gataatttat cttgtaaaac cttgcttgaa agtttcggca tggcataatc
    54361 tatcatagct ttaactaaaa ttttctggtc ttccgcaggc aaaatgcctt caaaaggaga
    54421 ttcttcaggc tgatctttta catacccttt aaacaggtca tattgttcat ttattttttc
    54481 gttagccatt gcgagcaaat gcaatcccag tttaaacaac tggttgatgc cttcattatc
    54541 ttctgtaatc ccagtttggg ctatctgcat ttgcaatgca gaagtcattg ctcttagaaa
    54601 atgattttta ggagttggtg ctgcattttt gaggtaatct gttaattgat tcagttgatt
    54661 tagttttaca tggtaatcac ttttccataa attcacatgc tgtttcattt tctcatattg
    54721 agactcaatc aggtctaatt tcattttaaa gggactaatt gatgaaacaa gccgcagcaa
    54781 ttcttggagt attagcgatc gtcgacttaa tgcatccaga tcattagggc attttccaga
    54841 tttgctggct tccaatattt cctcaaaacg ataatttttc tctgcccata tcaatacatc
    54901 ttggtaatct tctcttattt ttttcagatt attttcagga tctatagttt gcatgcatct
    54961 ggcgatatat ccgattttac cgactacttg actgtgatat tgctcgctag ttaagccacc
    55021 catttttata cccaccttga tata
//
LOCUS       DBNCWK010000006        52418 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.006, whole genome shotgun sequence.
ACCESSION   DBNCWK010000006 DBNCWK010000000
VERSION     DBNCWK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 52418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52418
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.006"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            293..823
                     /locus_tag="AC06IO_000302"
     CDS             293..823
                     /locus_tag="AC06IO_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HIO9873520.1"
                     /translation="MNKTITIALSLLLSITSKLTYAGPDRAQLAVWANEAIIATYTFD
                     YKNYMQQQKEIAKYFSADGWIAYSKALNQSKLPEVVQKNAYFVNAVATEPPKLITLDP
                     THWQAIMPILVVYKNPQYEQKQNLKVILGFTVASPGQGVRGFSVTSLQSTPISPPCQC
                     KIEETPGNTKQGDSKQ"
     gene            complement(798..1265)
                     /locus_tag="AC06IO_000303"
     CDS             complement(798..1265)
                     /locus_tag="AC06IO_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIO9873521.1"
                     /translation="MVWPIIWRWILDLGWLCFLLILFWYFWKKRRDLVEARSWLKAKG
                     HVTRCEWTRVGHSVWPRIEYIYEVYEKDLTGEYLFLDTTFNTPNSKYSRSIAYKVAIA
                     YRDNSEIDVYYNPNRPEQSALDVTIPKKLTFILILISSLILLHIGIIVWSLLV"
     gene            1400..2482
                     /gene="gcvT"
                     /locus_tag="AC06IO_000304"
     CDS             1400..2482
                     /gene="gcvT"
                     /locus_tag="AC06IO_000304"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIO9873522.1"
                     /translation="MTAKTPLHTTHLACGAKMVDFHGWDMPLHYGSQLNEHHAVRNDA
                     GMFDVSHMTIVDILGAGGRQFLRKLLTNDVDQITHNGKALYSCMCNEHGGIIDDLIVY
                     QRASDNYRVVLNSATRQNDVAWIRAKSEGFAVGLQERRELSMLAVQGPNAIAKTLSIL
                     APAHVDAVSTLTPFECVDVDHWFFARTGYTGEDGLEIIVPNEFVTQLWNDLLNAGVTP
                     CGLGARDTLRLEAGMLLYGQDMDETTTPLESGLTWTVKWEPEDRGFIGMGALVSQKQQ
                     GIKRKMVGLTLLDKGIMRHGQKVIIEGCPDGIITSGSYSPTLQQSIALARVPVETGEQ
                     VLVDIRGKLIPAKVGKPRFIKQGKPV"
     gene            2516..2893
                     /gene="gcvH"
                     /locus_tag="AC06IO_000305"
     CDS             2516..2893
                     /gene="gcvH"
                     /locus_tag="AC06IO_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIO9873523.1"
                     /translation="MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIG
                     DEVSAGQELGVVESVKAASDFYAPISGVVTAVNEAVGKNPALVNHDPYHEGWLVKLKP
                     SHPDEIKSLLSDEQYQNEIAEEN"
     gene            2896..4266
                     /gene="gcvPA"
                     /locus_tag="AC06IO_000306"
     CDS             2896..4266
                     /gene="gcvPA"
                     /locus_tag="AC06IO_000306"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIO9873524.1"
                     /translation="MPYIPHTPNDTKEMLAAIGAQDIQDLFDEIPASLQYAGFQNIPA
                     GINEMEMLKEAQNQAQKNRNGICFIGAGCYEHHIPAAVWDIASRGEFLTAYTPYQAEA
                     SQGTLQLLYEYQTMICELTGMEVSNASMYDGATALAEAVLMAVRLNKHSKTNRVLIAG
                     TVHPFYRETIETIVRNQHIEVITLPFDEQQGITDLGSLNQYTGEDITALVIAQPNFFG
                     CLEQVDKMTSWAHHNKTISVACVNPTSLALLKPPGSWGEHGVDIVCGEGQPLGSPMAS
                     GGPYFGFLSTRMAHVRQMPGRIIGRTVDKDGKTGFSLTLQAREQHIRRAKATSNICTN
                     QGLLVTAATIYMSLLGPEGLSQVATQCHQNTHELITALTQIEGVELAFKASFFHEALI
                     KLNQPVQSVLQQLADAGIAGGYAPEQHYPQLANTLLVCATEVRTAEDIAKYAKTLKAI
                     MSKRGA"
     gene            4266..4574
                     /locus_tag="AC06IO_000307"
     CDS             4266..4574
                     /locus_tag="AC06IO_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIO9873525.1"
                     /translation="MFIVQLTYLVPINEVNKYLQAHREFLDYHYKQGLLVASGPMKPP
                     TGGIIIAATNDRAHLESIFKQDPYYLAEIAEYQFIEFTPVKHRNELKELIQKTEGKLC
                     "
     gene            4568..6022
                     /gene="gcvPB"
                     /locus_tag="AC06IO_000308"
     CDS             4568..6022
                     /gene="gcvPB"
                     /locus_tag="AC06IO_000308"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIO9873526.1"
                     /translation="MLIFELSKTGRQAKAQIPRAVSKNYSIPEEFQRKSPPRLPACSE
                     LQVVRHFTCLSQKNFSIDTNFYPLGSCTMKYNPRGVHKAASLPGFINRHPLAMDNESQ
                     GFLETLYKLQNYISEITGMPGVSLTPMAGSQGEFAGVAMIKAYHQSRGDTARDEILIP
                     DAAHGTNPASAVMCGFKVVEIATAADGDIDLDELKRKVGPRTAGIMLTNPSTLGLFMR
                     QIKEIASLVHQAGGLLYYDGANLNAILGKVRPGDMGFDVMHLNLHKTFATPHGGGGPG
                     AGPVAVGKRLIPYMPLPVVKKTDSGYHWATRQDYPQSIGRLSCFMGNAGILLRAYFYM
                     LVLGKEGLLRVSEFATLNANYLLKELTKVGYTAAYPDRRASHEFILTLNSEKKNYDVT
                     AMDFAKRLLDYGVHAPTTYFPLLVPECLLIEPPETESKEELDAFVAVMKTIRDEAIKQ
                     PDILKAAPHTLPVKRLDDVKAARELDLNYFATHE"
     gene            6069..6434
                     /locus_tag="AC06IO_000309"
     CDS             6069..6434
                     /locus_tag="AC06IO_000309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873527.1"
                     /translation="MTVAAFYDGQSSLAKMCESLAKSMDYPNSHQAYTVGILSTLDGI
                     LNEPMPWLLAKIRLSEVLNEALLNYKGDLGKILKFAIGYEQANFNQLERIPVKSETLS
                     QSYLKGIEYANHVIDIINK"
     gene            6660..9881
                     /locus_tag="AC06IO_000310"
     CDS             6660..9881
                     /locus_tag="AC06IO_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873528.1"
                     /translation="MIIRELFNWIHNDSATLHLSDQTIERDLIEQEESQARQTHRVLL
                     GNVRLLKYSGSSNTEAIKELEQHCLFMDYLQLYKQEFVKDGKNSVCLIALRNLLSHSH
                     EEQLRLMPKINELYQILLRDNKESALSFYDKNKELFRHCTEIQEKVTFELHQQKMEAD
                     SKSVMTQLDYFNEHLAYQENPLGIIALCRNWIGETEKFTALILWLLERKVSVEKILLT
                     NLLQDFLKYHLFTLHSRDNEVSRLYSLLSHFPEAQELVMAAQKISCGELMFQQYSLDG
                     ILRGKNLPVVSPEIPPLQFSLSKDNFIALHRTFGPAFLTAGIATATNHGEVAWLDMLR
                     HTLNQPETLKLLPDIINIIAREYSPKVLKTLADLIAESTAHQLLTLNQSCVFHLLQHK
                     PRLLHDITEENVIEYINHLVRLDTHDQEIIYQLMALFRVLLKKNHPATKAVFEAILDN
                     LVNHPQLLEDEEFLTQLKKYPDCELLLEERCENILKQLNFCIAEQTSGLLFGKHNYFI
                     IEDVWLGALRKFAVLQQINPQMKFSFGHKYALQARIAEIVFIHQGDLFDLDTFIDALD
                     LPPVTSSGEISPYERALIEILATIDNDLIRKQIIQKLETAPFNRLDWHEREYGNQTVF
                     IKAAKKGNLGLINLLEDKIEPSVLNKALRTAAKNYQWETLDHLCSLPDVELRQDEMDD
                     LVVYLAEHGRIESVKKLLKLYDYKPSTELIGTILKKAIDNDNLQVVMYFCKLPVKSPK
                     QSMLDRLFKLAIQLQHWDIVEYLANSKHYSPSQTTLEKAFQQTALAMQHEAVEILGNV
                     EKTPVRAIVIERALLKASKLGHTKVVQSICALPSESLTKQAIEDALEQAAAEGHLDIV
                     SCLCESGTTTLRQPGINSGMKIAVQAGKLSVVNYFCSMTGSNKPTPRLIDQTLVMAAK
                     KGQTAIFIAIHSNHQTPPGKHAIEQSFQLAITAGKLPILDYLCRHEGYGLNQSKVDQA
                     LISAVKSKQQEIVSYLCESLEITPSRKVLRIAVSKAISSDQPGLAEYLRSRSMDKSKL
                     TDTLVDEIDSTSKVGNQLEANGLFKKKKRQTDREPQILFNLTI"
     gene            complement(10181..11218)
                     /locus_tag="AC06IO_000311"
     CDS             complement(10181..11218)
                     /locus_tag="AC06IO_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HIO9873529.1"
                     /translation="MVMRSFSFYYQHLENVSRSFSFCISQLTSPAKEWVALSYLLLRV
                     ADSIEDAKWQDLQTQSDSFAAFKSFLVQTPANDQFTKWLSSFPSQLPLGEKQLLCAVP
                     LLLEEKDNLPESIKQSVIKTILQVVDGMHYFLNQYHMEGKIVFPSLVTTNQYCFFVAG
                     LVGKLLSHIFTDLLSNFKWTASLLNQAFHFGFFLQKINLLKDKMDDETSGRYFISSEN
                     SLRESLVIHAHHSLAYIKGIPIIEGRPYRLFCAWSLFIGLASLKWLDKYGHRQKIKPR
                     ETYYLVNQINLLIDDNRALEKLFNSYLPGQYEEGSCYASESTKKIPNWFKKIYDGEMD
                     SLTLFDLDIEV"
     gene            complement(11218..13020)
                     /locus_tag="AC06IO_000312"
     CDS             complement(11218..13020)
                     /locus_tag="AC06IO_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIO9873530.1"
                     /translation="MIQLGDLLKPTWAAERSLNNAWSVLNDEEKETIKSRMDKIFYNE
                     IPFQLEHDKLIYIHLFSLFAQLETIGLRGLIKSLEKLRGTDLYQQMRQQITDEIFHAT
                     VFAKVAFQLSAPYALPLGHQKSINHFISSLEGEEDLATSITLVNLVGEGWVEELCVAM
                     KEKNIAVTIFATVLEDESRHMDEYDLYRQIGLPNKDYLRKKLAIFEDELINTVFAHEQ
                     YLTTLGILLGKEGALKLLNNINNKHHWMLKKIGLTPSAHWQLFMDTMPLLMKNLSHDF
                     EKDKAIEPTNIRKLLSAIWNDPELPTESAIFNINVTPVCFFEKKFKPETITCLMLQAL
                     SKACFDNPQTRNYIFNHKLYHSHNSYVALAVKIPGSDQLGAIEFKNCHEMTMTELAQH
                     IQHDMCIMMYCYEKTQSLQKEHPYLIEVVNRLLTPRHERVYRDFLFARPAISLSNIGH
                     WGYQAAVSPLFPNETFKITLTEIERKQVWNKTNNTFEVQDVLPVGMSVDHRVFDGNIP
                     FPRYMQEAFDQMFQDMEQSRIKPLSKPFSNLDSFIKYSNTLLENDLEFGFLYLFSLMH
                     VWKNYISYDELSKTVEENYERIKRALSKSEHQLG"
     gene            13626..13895
                     /locus_tag="AC06IO_000313"
     CDS             13626..13895
                     /locus_tag="AC06IO_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873531.1"
                     /translation="MNSKVFSQRFNRELATLGFPEDLTEKTKAVSKVFGVTRHLANAM
                     IFGHVLPDKEQLDKIAEILEVCPQWLSGATDRKKAYSGRETADSV"
     gene            13994..14800
                     /locus_tag="AC06IO_000314"
     CDS             13994..14800
                     /locus_tag="AC06IO_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HIO9873532.1"
                     /translation="MECLSFCVAKTIDLTRLDLHLKNVSKEFSAVKTRDVIRLNSHKN
                     KDHTLFIFKNGTVVSWGVKRYQIHEYLDIIKLLVDKPVALLVHDEFHYQIGDKTAIEP
                     HGFYDVDCLTIEEDSDELKLSLSYGFSQSVKLQYFETIIDALIEKYNPLIQALSHKGE
                     MPISRKQIQQVIGEILGAKSELNLISNFLYHPKYFWQHPTLEEHFSMLERYLHIQRRV
                     NAINHRLDTLNEIFDMFNGYLESRHGHHLEIIIIVLIAVEIIIAVMNFHF"
     gene            14818..16098
                     /locus_tag="AC06IO_000315"
     CDS             14818..16098
                     /locus_tag="AC06IO_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIO9873533.1"
                     /translation="MATLKSYFLKDTSLRTEILAGATTFLTMVYIVFVNPKVLHDAGM
                     DQGAVFTATCLITAFACALTGFIANTPIGVAPSVALNIYFSYSVVQGMGVDWQHALAM
                     VFISGLLFLFLTLTKLRRLLVEAIPHNLQIAIVIGISLLIALIALENNQIIIGDQHNL
                     MRLGPIIRPETGLFFLGFILILTLDYHKIPGAIIIGILSISVLSLMLGLTSWQGLIAM
                     PPSLEPTFLQLDFSGLSSIVALKTIFTFFLITVFDATGSLIGLLSPTIFKHEKNYYKR
                     LTRSLTADAAASSLAGLMGSASTSPYIESATGIEAGGRTGLTALVIATGFILMLFLFP
                     LAKMIPSFAVGPALLYVACCMIKHITNMKLQDITETAPCMVIIMMVPFTASIADGIGL
                     GIILYTLLKLFTRQKPNPLIIVLSLVFTVFFLIN"
     gene            16464..17060
                     /locus_tag="AC06IO_000316"
     CDS             16464..17060
                     /locus_tag="AC06IO_000316"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIO9873534.1"
                     /translation="MLEQSLRHYYQLVLVDPILPALRDRITPLSITWLSGIFGLLFIP
                     FVLLDKPYIAISFLLLSGYLDTLDGSLARFQNRASDFGSAMDIVMDRIVEFSAVLALY
                     LFDPQHRATLAILMLGSILLCITSFLVVGIFSNNTSQKSFYYSPGIMERAEAFLFFIF
                     MTLFSSCFNFLAALFCFLVCLTALIRLFEFKKSVRAPG"
     gene            17168..17743
                     /gene="msrA"
                     /locus_tag="AC06IO_000317"
     CDS             17168..17743
                     /gene="msrA"
                     /locus_tag="AC06IO_000317"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIO9873535.1"
                     /translation="MLRILLYFLLLINTTFCLAKTEKAIFAGGCFWCMEADFDHLPGV
                     LSTTSGFDGDTLKDPTYEQVSAGNTHYAESVMVEFDPNKLSYKKLVEYFFRHIDPTVK
                     NAQFCDHGSQYRSAIFYLSDQQKKIAMEYKKELEQKFPTVYTEIARSTSFYPAEEYHQ
                     NYYKKNPIRYKYYRYRCGRDTRIQEVWGNKK"
     gene            17862..18722
                     /gene="vipF"
                     /locus_tag="AC06IO_000318"
     CDS             17862..18722
                     /gene="vipF"
                     /locus_tag="AC06IO_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector N-acetyltransferase VipF"
                     /protein_id="HIO9873536.1"
                     /translation="MITQVNQLDELQLKDLKTLRAECKKNDGSIPNLYIHILKQHRSL
                     PTSFLYYQNGSLIGFLSVYFFYDDAVEVAVLVSPQYRRQGIAKQLIKEALPLIKSQNY
                     FNLIFSCPSRLNDNWLTQKGFTYLHSEYFMERDDLNPILDYIRPLSFRMATLEDIPIL
                     CGLDEVCFPDKNQDSVNRFQQILNEREYEIVIAMLNNHPIGKAHIRWQTKRATLSDIA
                     ILPKEQGKGFGSALIAHCINMILSEGKSRVDLDVETHNKKALNLYIQLGFHIQNACDY
                     WSINVNQLAK"
     gene            18847..20076
                     /locus_tag="AC06IO_000319"
     CDS             18847..20076
                     /locus_tag="AC06IO_000319"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIO9873537.1"
                     /translation="MKKRVVITGMEITSSIGSGIDKFWLAASQGQCGIKRIQSYDPSP
                     YSTQIAGEITDLSLDHLPEFNKSKRYPRAAQYALFCAHHAIEKAGLEQAELNAAGTFI
                     GTSIGGQPELEAGYRLFFTDSWKKIPPLSVIRGMPNSIANHIAIAFGIGGPNSTISNA
                     CVSSGEAIGMAYQQIAHGNLTTALCGGTESLVWESVMAAWCKLRVMSTNNENPSQASR
                     PFDLNRDGMVMADGAGILILEELQQAKARGAQILGEIIGYGASCDAFHVTAPNSQGQA
                     RAIVAALNDAKLSINDVHYINAHGTGTQLNDSTETETIKMVFGKRAYDIPITAQKAMT
                     GHAIGAAGAMEIIATTLSLQKNTLLPTINLYTPDPACDLDYVPNTAREKTIDIALSNH
                     FAFGGANAALVLRKYIS"
     gene            complement(20073..20855)
                     /locus_tag="AC06IO_000320"
     CDS             complement(20073..20855)
                     /locus_tag="AC06IO_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIO9873538.1"
                     /translation="MLKKILLAGLCVLFSMNLSASEPIYDEEIPLLWSSIITVSGGPS
                     WATPGQNQYIYPMSPVYINNFNPNLPPNYYLYFTHDSPTTILANGEIFFGLQRAINPQ
                     MIGQLGLGVAGVSDAKVTGDVNLNGLLNVYTYDYKVNHARVELKGKLIGSAFTPVQPY
                     LSGSFGIAFNNAHDYRTLSINQFLFPAPWFASNTTYAFAYSVGAGVQKMLNRNWQVGV
                     GYEFADLGKSYLGGDGVNITKGPRLTHLYNNQLLFSVSYLYS"
     gene            21119..22174
                     /gene="lpxD"
                     /locus_tag="AC06IO_000321"
     CDS             21119..22174
                     /gene="lpxD"
                     /locus_tag="AC06IO_000321"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIO9873539.1"
                     /translation="MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGE
                     DNIKLAENSEAAAILIGQGTIDSPKPLIQVKNPFKAFIALLNHFYPPRRISPGVHPTA
                     VIGAEVQLGDEVYVGPFVVIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYD
                     NCRIGSNVTIHASTVIGSDGFGYTFVDGQHLKVPHSGYVVIENNVEIGANTAIDKATL
                     GATVIGEGTKIDNLVQIAHSVKLGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVH
                     IEDEVILGARTGVPPHKHLKKGTVYLGNPAKPKDVAIKHELSVNRIPLIRKNIKSLTE
                     QVAVINKKLDIKAKEVE"
     gene            22177..24867
                     /gene="polA"
                     /locus_tag="AC06IO_000322"
     CDS             22177..24867
                     /gene="polA"
                     /locus_tag="AC06IO_000322"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIO9873540.1"
                     /translation="MKPPLILIDGSSYFFRAFHALPPLTNSKGQPTGAIYGVANMIKK
                     IIKDYQPEEIAVVFDAKGKTFRDEWYPEYKAHRPPMPQELSSQFQPLIQLLQAMGLPL
                     LIIDGVEADDVIGTLAKQATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDG
                     VKAKFGVTPEQIIDYLTLIGDSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLEHANEIG
                     GKIGEYLRASIPHLPLSKKLVTIKTDLDLPLNLNQLMPKPADSEQLIKLTRELEFKSW
                     LKELLQEEDNQKSPKEMDASNNKSYEIITTHQQLNHWLNKLEQSQQFCIDTETTNLDV
                     MQAELVGISLAVEEENASYIPLAHTDGSTQLVKEEVLTALKPILENPAIGKIGQNIKY
                     DYSVLKNYGITLKGIRYDTMLESYVLNSGAGRHDMDSLALKYLGYKTISYEDVAGKGA
                     KQLRFDQIPVEKAGIYAAEDADITLRLHHKLYPMLDKPLRNVLHDIEMPLLTVLADME
                     MHGVLIDPVILEKHGSRLKEQMKSLEQEAIQLAGKAFNLNSPKQLQEILFDEQKLPVV
                     AKTPTGQPSTAESVLQELAFDYRLPAVILEYRSLSKLVSTYIDALPKRINPKTHRVHT
                     SYNQAVAATGRLSSSDPNLQNIPIRSEEGRLIRTAFIAPEGSLIMAADYSQIELRIMA
                     HLSQDDNLLRAFANGWDIHAATASEIFQTNLDAVSKEQRRRAKAVNFGLIYGMSAFGL
                     AKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLGYVETLFGRRLYLPEINSR
                     NLMRQKAAERTAINGPMQGTAADIIKKTMLAVSNWEHSQKNPSAKMIMQVHDELVFEV
                     RKEAVEECRAIIHKLMEQTVKLSVPLVVSIGTGSNWDDAH"
     gene            complement(24897..25349)
                     /gene="lerC"
                     /locus_tag="AC06IO_000323"
     CDS             complement(24897..25349)
                     /gene="lerC"
                     /locus_tag="AC06IO_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator LerC"
                     /protein_id="HIO9873541.1"
                     /translation="MRVLIVEDSAFNAFCLRRLLESVVASISVTIVNNSQAALSFVNN
                     YCPDIVVVDGDLGASPDEMNCNGPELAGILLQKYPQLPIVAWSDSDAMREDFIKVYKS
                     HGLFLNEYNSWPKVIGLERIHKTWSYYFGEFIGGQNPSFASQTSINYY"
     gene            complement(25464..25805)
                     /locus_tag="AC06IO_000324"
     CDS             complement(25464..25805)
                     /locus_tag="AC06IO_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HIO9873542.1"
                     /translation="MNQLPNCPKCLSEYCYEDGDVLICPECTHEWPKNSNNNDEESAK
                     IIKDANGNLLQNGDTITVVKDLKIKGSSQVIKVGTKVKNIRLVDGDHDIDCRIDGIGA
                     MKLKSQFVKKI"
     gene            complement(25966..27159)
                     /locus_tag="AC06IO_000325"
     CDS             complement(25966..27159)
                     /locus_tag="AC06IO_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIO9873543.1"
                     /translation="MPKVIIFTDFDGTVTGRSGNETVFTEFYQSLLQGYQKDVEQDYK
                     STPMKDPIEVQALIEAKFGEYSENFDHSQQDVDFLMRPEAVAFFHEVLKNDDVTVNIV
                     TKNRVEYIKAVFKYQGFSDEEISKLTTLESGYKFNDVNSRLNHQTEKANRVYILDDSP
                     TDYAEMLRAVKGKGYNEEEIRGYRKNPGEFEWSQYLEDVREMFPPKVTPPEELSLPDE
                     RGDELSIGEEAPVLPQEDNLSRREETATVLSNQPVPSSDFRTLKIMGAFAGIGFGFGF
                     ALGVTLVATGVFAPSGVGLLGALTLGAISGTGAAALSSAIGFAVAEGTEITSEFVSEE
                     AVPAVKQSSHETMRSLGGSQLLSTNNIHPVGHFPSVLSRRTPTKGKEEQCQEEYVLTN
                     PLNNS"
     gene            27366..27533
                     /locus_tag="AC06IO_000326"
     CDS             27366..27533
                     /locus_tag="AC06IO_000326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873544.1"
                     /translation="MKTYEKAPTSLVGTLRYSISSILRDKDRVPLHMPRHRITLLGMN
                     STPPRPIPVPD"
     gene            complement(27542..28921)
                     /locus_tag="AC06IO_000327"
     CDS             complement(27542..28921)
                     /locus_tag="AC06IO_000327"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HIO9873545.1"
                     /translation="MDTHKVFVNRIINMRKIKLIGLDMDHTLIRYNSKNFESLVYDLV
                     KERLAESFHYPEEIKKFKFNFDDAIRGLVIDSKNGNILKLSRYGAIRLSYHGTKQISF
                     SDQKKIYRSIYVDLGDPNYMAIDTSFSIAFCILYGQLVDLKDTNPDKMPSYQTIAQDV
                     QYCVDKVHSDGTLKNIIIKNLKKYVIREKEVVEGLKHFIRYGKKIFILTNSEYSYSKL
                     LLDYALSPFLDKGEHWQGLFEFVITLANKPRFFYDNLRFLSVNPENGTMTNVHGPIVP
                     GVYQGGNAKKFTEDLGVGGDEILYIGDHIYGDILRLKKDCNWRTALVVEELGEEIASQ
                     IRALPIEKKIVEAMTIKKELEQKYVDLCTRSIDESSQQYDQEIHDLQLQISTVDLQIS
                     RLLQEQNSFYNPKWERVFRAGAEESYFAYQVDRFACIYMEKLSDLLEHSPMTYFRANR
                     RLLAHDIDI"
     gene            complement(28985..29635)
                     /gene="rpiA"
                     /locus_tag="AC06IO_000328"
     CDS             complement(28985..29635)
                     /gene="rpiA"
                     /locus_tag="AC06IO_000328"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIO9873546.1"
                     /translation="MSELKIKAAKAAIAYIEDDMVIGVGTGSTVNFFIKELAAIKHKI
                     EACVASSKATEALLRAEGIPVIDLNSVQDLPIYVDGADEVNERGEMIKGGGGALTREK
                     IVANVATQFICIVDESKVVKRLGEFPVAVEVIPMARSFVARQIVKLGGDPEYREGFIT
                     DNGNIILDVFNLNFSTPMALEDSLNVIPGVVENGVFAKRLADKVLVASASGVNNLK"
     gene            complement(29628..30377)
                     /gene="rlmB"
                     /locus_tag="AC06IO_000329"
     CDS             complement(29628..30377)
                     /gene="rlmB"
                     /locus_tag="AC06IO_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIO9873547.1"
                     /translation="MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLIKA
                     ENAHVPIEKLSAQKMNQQFSEFTHQGVVALASALPEYGESDLVTLLERSKKPVLILIL
                     DGITDPHNLGACLRSADATGVDFVVIPKDKSASITPVVSKVACGAAESIPLVRVTNLA
                     RAMDILKQQGVWIFGAAGEAEHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSL
                     PMMGSVESLNVSVATGVSLYEVIRQRGQFRE"
     gene            complement(30370..32550)
                     /gene="rnr"
                     /locus_tag="AC06IO_000330"
     CDS             complement(30370..32550)
                     /gene="rnr"
                     /locus_tag="AC06IO_000330"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIO9873548.1"
                     /translation="MSKKSKDPFYKREREKYAEPIPSREYIMEILDEYGRPMSRSKLF
                     DKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINLLRGTVQGHPDGFGFF
                     IPDDGSEDMVLSAKEMRAVMHGDLVLAYQSGLDRRGRPEAKIHEVLEHANTTVVGRFF
                     TDHGVNFVIPDSKRLTQDISIPQEFIGEAKNGQIVLVELIAYPNKRSQAIGRVIHILG
                     EHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLRNLPFVTIDG
                     EDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVYFPGKVVP
                     MLPEALSNGVCSLNPHVDRLCMVAELSISQEGKISRSRFYRAVIHSHARLTYTSVGAW
                     LEQGKADEKHQALWPMLESLYDLYHVLLDTRRLRGAMDFETTETRIEFDENKKIKCIV
                     PVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELGL
                     SLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYT
                     HFTSPIRRFPDLLIHRAIGHLLDNHPIYEFEYSHEDMNRLGKHSSMTERRADEATREV
                     ITWLKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVHVTSLKNDYYTFDSAK
                     HRLIGARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE"
     gene            complement(32946..33410)
                     /locus_tag="AC06IO_000331"
     CDS             complement(32946..33410)
                     /locus_tag="AC06IO_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873549.1"
                     /translation="MRILLSFYLLLLSTGLFAESTFPEGCRAVPVQGQSVTLKAKKNK
                     LVFIHNITNLDLWITHPVTDPGASAGWTSRLQAGKWSALVVDKPAFILNCIESRPGHE
                     QQIPCENAIAVCQWKGVKIPASNQGTFWVAEDLSLAALNAAVGGRGFVLPEK"
     gene            complement(33631..37605)
                     /gene="lem1"
                     /locus_tag="AC06IO_000332"
     CDS             complement(33631..37605)
                     /gene="lem1"
                     /locus_tag="AC06IO_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem1"
                     /protein_id="HIO9873550.1"
                     /translation="MKQLISIIKGQIQQLPIPDLKHLLLSHLKEDAHDLTELFCQQIP
                     AQKNQFSLNHSVPFGLPASLSVLAIVLTYAPNLLHRELSEQLLTMLDSIKETKTSVDN
                     QLAERCTKQLNYYSDAKPVLIALKERGAKDLIQILEQADERSLSAFSHLAKDTDLNVL
                     IETFLVKNNKDLDLVNRNHLVQFLRQPDLAGWLTRFLDSLTDESINALSIRLLLSLVS
                     SEKAIEKLKEARACLAQYPFSIDLHAMRAVFDFYQYELKRDLFSPESHLVMQLLHDLL
                     KNTKLDKQQEFFQGLSQETVLLIIEYCIAEYNKTTGEEKQNCKNLLTRLCSAFSDSSQ
                     DFLGEIRTKFNQLDPYLFDTPQLYQLASDLITKSCNSGKEPLSSSPIALLLSMPQFVG
                     VCTPKVLKQLVERYSLYTLTLSQKDYKQDLCFKEYAPYFDEINEHMAQIERNLLIHPE
                     AQSFWLDKKYSLLQFRVEISKSALLHQMECLLEANKGGDELHNKGLEVLYHYYALSYP
                     NLRSDLFLKVVNHIYSRFFIKGNNETEVRNGLFHWLRQSFPLIDKLPKELDRKKEIFL
                     YNSAGKKIGFLGESNAAMTFVNEEIVLLARTGSMDINEPLYDQEGLVLGYLTEESCLR
                     SESAIHKNAAAWVLAGLSKSDLESSSFGLESLLHNVLFENAIGVLYQCESICLDANKR
                     IWLNNKLLKEIQEAPGEIPASVLYPLLDHFEDETVFELLGAITNKNNALELFHYILNQ
                     DPKRSILLNGLYESDFQRFLEHQGADVCLADYLVNYYDKPWFSEVLLRFAYHGKKYKK
                     PDLLSSALTFITKEISQDKKQETICDAVLDRLINSEACAALVLKEFLGDRTHNSIQKV
                     NNPEIGKVSQFFDRRHIMPLIAQLNKTSYWEQNAQYKLALHILANQHGRLFSETDLNP
                     DSPQAWQGKELKELACFTSRHLSKKRPFDNKHDIGYRVLGELLFRCANSGITSLFYKQ
                     KALNRAITRLSITEPFLVRMVDKLWMPKGIRELYGKSLFFKGSWFDDQVFLQKELKDH
                     HPLLDWSHFIKQTWSKRDKQKKSPIICSYLLSYTGKKSVLLHLLQDYFNSFQGMSDYI
                     RPVSQLLRFFPQRNVSAVIYDALEAEMIKKPQLLDRQILSDMAFYYGKQLARKDTSFP
                     QADINLLTYWGQNKHYSLAKRGCELLARDCEDKGVKKRLVSAAKEAEVEQDLSSSVQT
                     FYFGLIKFFKRLWHYGFNAENSSSKIIKFCDDPTPGPTRKKAGDFINAPVAESRTNSL
                     HLGFSERRKQLISLLATIKSSPAHFTRVVNYSDNTQTFFGEQPKQSIDYNTPAIPSNF
                     ESIM"
     gene            37716..38162
                     /locus_tag="AC06IO_000333"
     CDS             37716..38162
                     /locus_tag="AC06IO_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIO9873551.1"
                     /translation="MTIWVDADACPKMIKDILFRAAIRTNTNLILVANSYLTYPNSPF
                     IRSILVEKGYDRADHYITSHMKAKDLVITADIPLAAEVIVKQGLAMSPRGELFTANNI
                     KQRLTLRDINEQLRSAGERTGGPSALSAKEKTSFANALDRWLAKNK"
     gene            38252..38980
                     /locus_tag="AC06IO_000334"
     CDS             38252..38980
                     /locus_tag="AC06IO_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIO9873552.1"
                     /translation="MKRFMFILLIFINFHAFGKALIIGVPDFAPPFVSRSGGGEFYFG
                     FNIDLMNSICKIIEEECQYKGMNIQQLYTNLNTGKVDIMLAPTPIVSTAGTNYIFSLP
                     YLPSNAQIVTLKANDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLPD
                     MAIALSNREIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMK
                     INKALLQIEKDGTYLEIYNTYFGD"
     gene            39207..39797
                     /locus_tag="AC06IO_000335"
     CDS             39207..39797
                     /locus_tag="AC06IO_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HIO9873553.1"
                     /translation="MQESFDPSTITTLHKTSIIRWKANGLEYQHKQFLQLVEENHAFN
                     FQLWHAEDKARRDDMGFEFVYQAKREIDAFNQQRNNRMEAMDEWLYKALQPENHNHCP
                     VNSESPGMIIDRLSILSLKSYHMDLQTKRQDVGVEHRIACTQKLEIIQQQLNQLALCL
                     DELLNEVRAKTRTFRVYHQFKMYNDPKLNPELYCSD"
     gene            complement(39763..41688)
                     /locus_tag="AC06IO_000336"
     CDS             complement(39763..41688)
                     /locus_tag="AC06IO_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873554.1"
                     /translation="MPHASDENQASKPTDLVFILAIAGRIDAQSRILAQKLDKSRSIY
                     YAYAVMDSLSSSYSMFKYFFDLFASPNDNDAMHEFLNSPAGLITIAVESVFLVTFSVL
                     ACQFENEKEDYYKKFIANAWPYFRDVMKGLKNAYKGWRSAVVAFSLIGGVDAKFLILP
                     MGVILGVFAAANRFWIRSMLEDRKTMMSENIALLMEIKKLISLSHEESFSYFKRIRYQ
                     TMQTRNFAFWAVAAGGLLDGLYLYVGVLSLAVLSPQLLMAMALICAFYTVACVITRIY
                     EEYDFQLRLLVTQTKCQLALITKELETNYAKLLLLQDKMDLDKVDQEELKRLKRLVHN
                     LITRFDDNRKLLSQQSNRTYLSSALLGMKNGLYAYGALASVLFLVGSILVMAGVSFPP
                     ALLTACIILGLVLVLSFTIHSLVSNYWHQTKQETQKQRPYERLIEWNNCLNDEFNKTE
                     LLAPEEFHKLLNDGLSLDPSPQFFFQEWFEVLRSLFSGFGKGQKFIDFAGNSLQELGE
                     DGHYRDTPVMYVLSVFSALLFGVTLALRALAKGLGRTPLAQQAKLTEDSKEASVSPEI
                     TKEVMQKTEEQRESIQSEKTRTDSPGHTDSLSLFGIFETKTKRNQPDSPSIPHSKSES
                     ALNQLIPSQNSTILGLA"
     gene            41938..42465
                     /locus_tag="AC06IO_000337"
     CDS             41938..42465
                     /locus_tag="AC06IO_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIO9873555.1"
                     /translation="MRSKFVLFMLMSLLTGSLMANSEAQIYGYVEKATLIDQNLTLSA
                     KLDTGAKSASLHAVNITEIEKKGIPYLRFTVPTKTGDYSFEGEYVGKVKIKVRSSEAN
                     PGLLRTTPIKRPVVLLNIKLGDKVRTIKVNLTNRKRFLYPLLLGRDAIIDFNGAVDPA
                     LTFTTKSKSPVNNEK"
     gene            42455..43978
                     /locus_tag="AC06IO_000338"
     CDS             42455..43978
                     /locus_tag="AC06IO_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HIO9873556.1"
                     /translation="MKNNKRHVYGLILTLLLLGSGIFLYRHIILDVPLTDTETINSWM
                     VESNLRFTADRNTPIKASFNIPYLPPNFAILDEYFVSRNYGVTTNLNGSNRETVWSIR
                     RGHGPQSLYYRAIFRQTDSDESSLPKPSVTKSQPLNDSQKSAVETITNQVRSTSADIQ
                     TFAQSTIKELNKRDGNAKLLVGNEFNDDNIINATILILNQSKIPAIAVQGIYLNQQKK
                     ADLKSLLAVFNGKNWIYINPKTGSAGLPKEFLIWQYGNGPLFNVVGGNRAQFSLTVSP
                     TPINALSVAKSRGLEDSQLLRFSLLQLPVNVQGIYKILLTVPIGAFIILILRNFIGIK
                     TFGTFMPVLIALAFRETHVAWGITLFVIIISFGLLARFYLDQLRLLLVPRLAAILTVV
                     ILLMIFISVLCQNLSLDTGMSVALFPMVILTMTIERMCITWDERGASEAIKSGIGSLA
                     AAVISYGAMSYEPLQYLIFAFPELLLVLLSLILWFGQYRGYRLVELKRFKSLASAMK"
     gene            43975..44922
                     /locus_tag="AC06IO_000339"
     CDS             43975..44922
                     /locus_tag="AC06IO_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HIO9873557.1"
                     /translation="MINLFRRLKNHGVLSINQRNTDFVLRYNPRKLFPLVDDKLKTKK
                     LALKAGIAVPALYDIIETEQQIKSIETRFASYNDFVVKPARGSGGDGILVFKDKVYGR
                     YRQINGKLTTVQELSYHLSCLLSGAYSLGGSSDYAIIEKRVVVDPVFAEVSYEGIPDI
                     RIITLLGYPAMAMVRLPTRLSGGKANLHQGAIGVGVDLATGKTLGGVFQNDTIDYHPD
                     TLNPIVGIEVPYWNQILEIASSCYELTGLGYLGVDIVLDKEHGPLMLELNARPGLNIQ
                     IANREGGLKRYRTIEARFKENGKESTLDKVNFCRKEFAR"
     gene            complement(44919..45377)
                     /locus_tag="AC06IO_000340"
     CDS             complement(44919..45377)
                     /locus_tag="AC06IO_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873558.1"
                     /translation="MFGRLWLRRPACLPTLDLSKMVLGRMPAETTFESIKQEFKQELH
                     RVGLKRFQENIHAEKLILEQLLRQREELTKKIDEECLNPALRRCANGHDVKGLFWSNM
                     QGNLAKIQSKKERVEMDEMIDWLCARRRFLKEWNNLSLDKELNCSALPTL"
     gene            45662..46987
                     /locus_tag="AC06IO_000341"
     CDS             45662..46987
                     /locus_tag="AC06IO_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HIO9873559.1"
                     /translation="MRSKLVSFFSRSSRPMKAIPSKIWFETQLKSSGLDKKFSIDELR
                     ETQSSVRVFANKKYLPDTQTINEALTKVTAINVSGDKSGYFQNGLPFPNEAGYFEKIP
                     AGHPELLSPIERLASSKKVVSSHSLVTASGGYPLTNPLLPYRKPIKVSIFSLAGPSFE
                     NNYLHYRLFLLDPIQKIIDSPLFSHLHDGLPIHFDDAKKELGGYDTNKVMARIRLGFP
                     YLARFSSGGFYPSFSKSNAIIFLSEAYFRYQLEDISLLLASVNQTAKETGKAAFLKAT
                     AVGMGFFAKIDCGYDIQHMIFPYYLRAYKKLLSEHKFPWIAKIEFPIFNEIQQEQFDS
                     IFEDYNGSTKVYQSTRDVLEFREDEIEKYFPAAINPSDAFALTGNEWGYGSVESMIGN
                     NSSIRFDQVHHMNPLILDPSHHVAVQINKDHRVELSANAMPQLSSSIKP"
     gene            complement(46977..47744)
                     /locus_tag="AC06IO_000342"
     CDS             complement(46977..47744)
                     /locus_tag="AC06IO_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873560.1"
                     /translation="MTRLKFFSKFQKALLQLETSVSNLSNPLVGSKIKELQTKKILVR
                     ADGRSLDHLHKLGGLPQRVESEKLEKVRITDVERYQKFNMNPFGWGACASAEDLRKFL
                     ENYSELTKQAWVHKFYGSSTSLLTLKSEVGISCGDHDEEKEELVVDSVSFEQIIASTC
                     PKYREKYLGGGLVPNAMKDFKSKVPHTMRLGDFSSEKSTLEWLLINDCEEEFAKLCKE
                     IYGDTPLKILLMRFEGDKYLADAVTYYVKEAASSPRV"
     gene            complement(47920..49083)
                     /locus_tag="AC06IO_000343"
     CDS             complement(47920..49083)
                     /locus_tag="AC06IO_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HIO9873561.1"
                     /translation="MSQHFDVLVIGGGIVGLTAALAMAQRHYTVAVIDAGTLMVNTDK
                     SDLRVYAINKASKMLLSELGVWQHLDDARVAPYNKMHVWDGVSGAYIDFDSRSIAEPN
                     LGAIIEESILKQALLRRISSESKISLFPHSVIDEVCHLERSIKISGQNNAWEGQLLMI
                     ADGANSPMRHKLKVDVTSWSYEQQALVATVNSEKSHQNTAFQVFHPDGPLAFLPLSNL
                     NQCSIVWSTHADNAMRLKLLDDNEFSRELTKAFGGQLGQIKVVSSRHQFPLQMRHVKQ
                     YAGHRWLLLGDAAHTIHPLAGLGLNVGLADVKSWLTCLEMGKGELISKKVLGAYQRER
                     KHSVWQIILLMEGFKRLFGTSASPVTSLRSLGLRACNEFIPIKRLFMRQASGL"
     gene            complement(49080..50282)
                     /gene="ubiH"
                     /locus_tag="AC06IO_000344"
     CDS             complement(49080..50282)
                     /gene="ubiH"
                     /locus_tag="AC06IO_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HIO9873562.1"
                     /translation="MANKETDILIIGGGLTGASLSLALQGLGFSVTLVESRPYSDKVN
                     PDFDARSLALSPASQRILAMLGVWEILKEYTTPIHMIHVSDQHRFGVSRLQGEKNSPL
                     GYVVEMQYINRALSILINKEHLLAPAVLKSIDFNNSTATVAHGDDMISIRAQLIVAAD
                     GTESMTRHLCNLPVKIKDYAQDAIVANVGLIKPHGNRAFERFTPNGPLALLPMMNDKM
                     SLVWASKPEEAKRLVMLNDGDFLRELQTAFGYRLGRFGKVGRRYSYPLKQVLMPQQTK
                     WPVVFVGNAAHTLHPVAGQGFNLGLRDIAALAQCISQKGLNPQMLEHYLKLRAHDQQM
                     ITWLTDGLIHLFTSRLPGMGVARNLGLLALDNIPALKNLLARYARGFGGITPDLVCEI
                     ALSAGEFQ"
     gene            complement(50272..51582)
                     /gene="pepP"
                     /locus_tag="AC06IO_000345"
     CDS             complement(50272..51582)
                     /gene="pepP"
                     /locus_tag="AC06IO_000345"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HIO9873563.1"
                     /translation="MISQQEYKARRKKLAAQLPDDSIAVIPAAHEVLRSGDTHYRFRQ
                     DSDFYYLTGFNEPDALLVIIAGKDSQSILFNRPRNPAEEQWTGKRLGQDGALQELGVQ
                     AAYPLSSITEELPKLFSGKSAIYYDLGRNLLLEKKIRHVLSSLKAQVRRGIKAPESLC
                     DLEPILSEMRLLKSDAELELMRQAARISVAAHKRAMQVCQKMEYEYQLEAELIYEFSR
                     HGCRSVAYDPIVGGGANACILHYTENNKPLRRGDLVLIDAGGEYGSYAADITRTFPVS
                     GTFSPEQKIIYELVLKAQKAGIAAIEPGLPWNHIQQIIIRTLTEGLCDIGILKGDLEE
                     LIKREAYKPFYMHNSGHWLGLDVHDVGRYKINNEWRPLEAGMVLTVEPGLYISAGMEG
                     VDKRWWDIGVRIEDDILVTRAGYEVLTGDLPVNVDDIEALMRGE"
     gene            complement(51687..52268)
                     /locus_tag="AC06IO_000346"
     CDS             complement(51687..52268)
                     /locus_tag="AC06IO_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HIO9873564.1"
                     /translation="MSLDNDSLHLPKYDDFVESISVLALTMSGSELHGIMCGYLCAGA
                     DSQGEAYIRALLNNKKDEQSRNALLSMFSVFSISQQQMNNFDFEFEMLLPDDDESLVT
                     RAQAFSEWCEGFTQGLTIAGVGMEQFYEEESQDALQHLMEFAELDCETLEVGEEDERA
                     LMEVSEYTRMAVLRLHSDLVLHERELGDSGTTH"
BASE COUNT        16408 a        11007 c         9521 g        15482 t
ORIGIN      
        1 gaatataaaa taccataaat acaacgtcat cgataccaga caaaccaaca cattcaaccg
       61 tatttacaaa taattttgat taaattttga acttttttgc gattcatgtg tctaatatag
      121 tgagcattct cccccgagtg ctctaccccg ctttccccct ctttatgcgg ggtaatatcc
      181 ggattgcacc aaatccggat atataattct ccccgggatc ttagccgggg atttttttgt
      241 aatgaaaaaa tgactaaact ggcagtattg atataatcga tgagaattta ttatgaacaa
      301 aacaatcact attgccttga gtttacttct aagtattacc tcaaaactaa cttatgcagg
      361 gccagataga gcacaattgg cagtttgggc taatgaagcg attattgcta cttacacttt
      421 tgattataaa aattacatgc aacaacaaaa ggaaatagcc aaatattttt cagctgatgg
      481 ctggatagcc tatagcaaag cgcttaatca atcaaaactg cctgaagtag tacaaaaaaa
      541 tgcctatttc gtcaatgctg ttgccacaga accacccaag ctgatcaccc tggatccgac
      601 acactggcaa gctatcatgc ccatactggt cgtatataaa aacccccaat acgagcaaaa
      661 acaaaattta aaagttatat tagggtttac cgttgcatct cccggacaag gtgtaagagg
      721 tttcagcgta accagcttgc aatccactcc catcagcccg ccatgccaat gtaaaattga
      781 agagacgcct gggaatacta aacaaggaga ctccaaacaa taataccaat atgcagcaaa
      841 attaatgagc tgattagaat taaaataaaa gttagttttt taggtatggt aacatctaaa
      901 gcagattgtt caggacgatt gggattatag tagacgtcaa tttctgagtt atctctgtaa
      961 gctattgcca ctttatatgc aatacttcgg gaatatttac tgttgggtgt attaaatgtt
     1021 gtatccaaaa acaaatattc ccctgtcaaa tccttctcat aaacttcata gatatattct
     1081 attctaggcc aaacactatg ccctactctt gtccactcgc agcgcgtgac atgccccttc
     1141 gcttttaacc aggatcttgc ttcaaccaaa tctcttcttt ttttccaaaa ataccaaaaa
     1201 agaattaaaa gaaaacacag ccatcctaaa tcaaggatcc atctccaaat tatcggccac
     1261 accatattct ttcccataaa tttatattat atgctatttc cagggagagg atcatgcaaa
     1321 aaaatcaaat tggaaacctc gcaaaattaa tcccaacagg tataatcaca tccaataagt
     1381 gataaaaaaa ggaaatgcaa tgactgctaa aactcctctt cacactacgc acttagcatg
     1441 tggtgccaaa atggtggatt tccatggttg ggatatgcct cttcattatg gatctcagtt
     1501 aaacgaacat catgctgtta gaaacgatgc tggcatgttt gatgtctcac atatgaccat
     1561 cgttgatatt ttaggcgccg gtggccgtca atttctgcgc aaactgctaa ccaacgatgt
     1621 cgatcaaatt actcataatg gaaaagccct atacagctgt atgtgtaatg aacacggcgg
     1681 aattatagat gacctgattg tatatcaacg cgcttccgat aattaccgag tggtacttaa
     1741 ttcggcaacc aggcaaaatg atgtggcctg gattcgcgca aaaagtgaag ggtttgctgt
     1801 tggcttacaa gaaaggcgcg aattatcaat gctcgctgta caaggtccaa atgccatagc
     1861 gaaaacattg agtattcttg cacccgccca tgtagatgcc gtttcaactc ttaccccttt
     1921 cgaatgcgtt gatgtggatc actggttttt tgctcgcacc ggctatacgg gggaagatgg
     1981 ccttgaaatc attgtcccta atgaatttgt tactcaactc tggaatgatc tcctgaatgc
     2041 tggagtaacg ccatgtggat taggggccag agataccctg agattagagg caggaatgct
     2101 tctttacggc caggatatgg atgaaacaac aaccccactg gaatctggtt tgacctggac
     2161 agtaaagtgg gagccggaag atagaggatt tatcggaatg ggcgctttgg tttctcaaaa
     2221 gcaacaagga attaagcgta aaatggtcgg tttaaccttg ttggacaaag gcattatgcg
     2281 tcatggccaa aaggttatta ttgaaggctg ccccgatgga attatcacaa gcggcagtta
     2341 ttctcctacc ctgcagcaat ctatagcttt ggcacgagtt cccgtggaaa caggggaaca
     2401 agtacttgtt gacatacgcg ggaagttaat ccctgcaaaa gtggggaagc ctcgctttat
     2461 caaacaagga aaacctgttt aaattttagt aatattaata ttaaggatat aaaaaatgaa
     2521 tgatttgaaa ttcacaacaa cccatgaatg gctgagagaa gatgaggaag aggtcacagt
     2581 aggaattaca gatcatgcgc aagaattatt gggggatatg gtatttgtag aactacccga
     2641 aataggtgat gaagtgagtg caggccaaga attaggcgtg gtggagtcag tgaaagcagc
     2701 ttctgatttt tatgctccca ttagtggtgt tgtaacggcg gttaacgaag ccgttggtaa
     2761 aaaccctgcc ctggtaaacc acgatcccta ccatgaaggc tggctcgtga agctaaagcc
     2821 cagccatcct gatgaaatca aaagcctgtt gagtgatgag caatatcaaa atgagatagc
     2881 tgaggaaaat taatcatgcc ttatattcca cacacgccca atgataccaa agagatgcta
     2941 gcggcaattg gtgctcaaga cattcaggat ttatttgatg aaataccagc ttctttgcaa
     3001 tatgcaggat ttcaaaatat ccccgctggc attaacgaaa tggaaatgtt gaaagaagcc
     3061 cagaatcaag cccaaaagaa tcgcaacggt atatgtttta ttggtgctgg atgttatgag
     3121 catcatattc cagcagccgt gtgggatatt gcatctcgcg gtgaattttt aactgcctac
     3181 acaccctatc aagctgaggc cagccaggga actttgcaat tattgtatga atatcaaacc
     3241 atgatctgtg agttgaccgg catggaagta tccaatgcgt caatgtatga cggagcaact
     3301 gcgttagctg aagcagtact aatggcagtc cgccttaata aacacagcaa aaccaacagg
     3361 gtactgattg caggcacagt tcatcctttt tatcgcgaaa ccattgaaac gatagtacgt
     3421 aatcagcata ttgaagtcat caccctccct tttgatgaac agcaaggaat cactgatctt
     3481 ggctctctca atcaatacac cggagaagat attacagctt tggtcatagc ccaaccgaat
     3541 ttctttggtt gcctcgaaca agtcgataaa atgacttcct gggcacatca taacaaaaca
     3601 atcagtgtag cttgtgttaa cccgacttca ttggctttat taaaaccacc cggctcatgg
     3661 ggagaacatg gggtagacat cgtatgcggt gaaggacaac ctttaggctc acctatggca
     3721 tcaggaggcc cttattttgg ttttctgagc acccgtatgg cccatgtcag acaaatgccc
     3781 ggaagaataa ttggccgcac ggtagacaag gatggaaaaa ccggttttag tttaactctc
     3841 caagcaagag agcagcatat tcggcgtgct aaagccactt caaacatatg caccaaccaa
     3901 ggtttacttg taaccgcagc aactatctat atgagtcttt tagggccgga aggtctaagc
     3961 caagtggcaa ctcaatgcca tcaaaacact catgaattaa ttactgcctt aacacaaatc
     4021 gagggggtag aactggcatt caaagcttcg tttttccatg aagccttgat taaactaaac
     4081 caaccagttc agtccgtatt gcagcaactg gccgacgctg gaattgctgg aggttatgca
     4141 ccggaacaac actaccctca gctagccaat acgctgttag tttgtgcgac agaggtgcgc
     4201 actgcagagg atatcgcgaa atatgcaaaa acattaaaag ccataatgtc caagcgaggt
     4261 gcctgatgtt tattgtgcaa ttaacctatt tagttcctat caatgaagta aacaaatatc
     4321 tgcaggcaca cagggagttt ctagattacc actacaaaca agggttattg gttgcatctg
     4381 gccccatgaa acctccaacc ggtgggatta ttatagcagc gacaaatgac agggcccatt
     4441 tagagtcaat ttttaaacaa gacccttatt atttagcaga aattgctgag tatcaattta
     4501 tcgagtttac tccagtaaaa cacaggaatg aactcaagga acttattcaa aaaacggaag
     4561 gcaaattatg ttgatttttg aattatctaa aactggtcgc caggcaaaag ctcaaatacc
     4621 cagagcagta agcaaaaact attcaatccc cgaagaattt caacgaaagt cgcccccaag
     4681 attgccggcc tgctccgaat tgcaagtggt aagacacttt acctgccttt cccaaaagaa
     4741 tttttccata gataccaatt tttatccttt gggatcatgt actatgaaat acaatccccg
     4801 gggtgttcat aaagcagctt cgcttcccgg ttttataaat cgccatccgt tagccatgga
     4861 caatgaaagc cagggatttc tggaaactct ttataaatta caaaattata tttctgaaat
     4921 tacggggatg cccggtgttt cactaactcc aatggcaggt tcacagggag aatttgccgg
     4981 agtcgccatg attaaagcct atcaccagtc tcgtggcgac accgcccgag atgaaattct
     5041 cattccagat gcggcgcatg gaacaaaccc cgcctctgcc gtgatgtgtg gcttcaaagt
     5101 agttgaaatt gctactgctg cagacggtga cattgatctt gacgaattaa aaagaaaagt
     5161 aggccccagg actgccggaa ttatgctaac taacccctct actttgggat tatttatgcg
     5221 tcagataaaa gagatagcta gtcttgtaca ccaggccggg ggattattat actacgatgg
     5281 cgctaatctc aatgctattt taggtaaagt aagacctggt gacatgggtt ttgatgtcat
     5341 gcatcttaat ttacataaaa catttgctac acctcacggg ggtggtggtc ctggcgctgg
     5401 ccctgttgct gttggcaaac gccttattcc ttacatgcct ttacctgtag tgaaaaaaac
     5461 cgactccgga tatcactggg caactcgcca ggattatcca caaagtatag gaagattatc
     5521 gtgctttatg ggtaatgccg gcattttatt acgtgcttat ttttatatgc ttgtccttgg
     5581 taaagaaggc ctattgcgcg tatcagagtt cgcaacactt aatgccaatt atttacttaa
     5641 agaattgact aaagtgggct acacagcagc ctatcctgac aggcgtgcat cgcatgaatt
     5701 tattctcact ttaaattccg aaaagaaaaa ttatgacgtg actgccatgg attttgcaaa
     5761 aaggttattg gactatgggg ttcatgcccc cactacttat ttccctttac tagtaccgga
     5821 atgcctgttg attgaaccgc ccgaaacaga aagcaaagag gaattggatg cttttgtagc
     5881 cgtgatgaaa accattcgcg atgaagctat caaacaacct gatatactca aagctgcgcc
     5941 ccacacttta ccagtcaaaa gactggatga tgtgaaagcg gctcgcgaac tggatttaaa
     6001 ttattttgca actcacgagt aacctgtaca acagattttt ccaattttag cgcagcagct
     6061 ttcttttgat gacagttgct gcgttttatg acggccaatc ttctctcgcc aaaatgtgtg
     6121 agtccctagc aaaatcaatg gattatccta actctcatca agcttatacc gtaggcatac
     6181 tttctacttt agatggtatt ttaaatgagc ctatgccttg gctattagca aaaattcggt
     6241 taagtgaagt actcaatgaa gcgctactga actacaaagg agatttaggc aaaatcctga
     6301 aatttgccat aggttatgaa caagcaaatt ttaatcaatt agaacgcatt cctgtaaaaa
     6361 gcgaaacgct ctctcaatct tatcttaagg gaatagaata cgccaatcat gtgatagaca
     6421 ttattaataa ataattgtcg tccctattag tattcaatct gttttacaaa agacaatact
     6481 atggaaatta tccatatcag atagtacaaa aaggattttc ataccacgaa taactcacta
     6541 aattttattt aaaatcacaa agatcttaaa ttgtccttat cgatcaaata atagcttgat
     6601 gtttcctctt gtttatttat aattcatgga atgttaaatt tattctctta ttgcaaatta
     6661 tgatcatacg tgaactgttt aattggatac acaatgatag cgcaaccctt catctcagtg
     6721 atcaaacgat cgaacgtgat cttattgaac aagaggaatc gcaagccaga caaactcatc
     6781 gtgtgcttct gggtaatgtc cgtcttttaa aatatagtgg ttcatccaat acagaggcga
     6841 ttaaagaatt agagcagcat tgtcttttta tggattatct ccagttgtat aaacaagagt
     6901 ttgttaagga tggaaaaaac tctgtttgtc taattgcatt aagaaacctg ttatcccact
     6961 ctcatgaaga gcaacttcgt ctcatgccta aaattaatga gttataccaa atattattac
     7021 gagacaacaa agaaagtgct ctatcttttt atgataaaaa taaggaatta ttcaggcact
     7081 gtacagaaat tcaagaaaag gtaacgtttg agcttcacca gcaaaaaatg gaggctgaca
     7141 gtaaatcagt aatgactcag ctagactatt tcaatgaaca tttagcctac caggaaaatc
     7201 ccttaggaat cattgcgcta tgccgaaatt ggattggtga aacagaaaaa ttcactgctt
     7261 taatactctg gttgctagaa cgcaaagtaa gcgttgaaaa aattcttctt actaatctgc
     7321 tgcaggattt tcttaaatat catttattca ctttgcattc cagggataat gaagtttctc
     7381 gcttatactc cctactaagc catttccctg aagctcagga actcgttatg gcagcccaaa
     7441 agataagctg tggagaatta atgttccagc aatactctct tgacggcatt cttcggggta
     7501 aaaatcttcc tgttgtttca ccagaaatcc cccctttgca attttcctta agcaaagaca
     7561 attttatcgc attacaccga acttttggcc cggctttttt aactgcagga attgctactg
     7621 caacaaacca tggtgaagtg gcttggctgg atatgttaag gcatactctg aatcagccag
     7681 agacactcaa attactgccc gatattatca atattattgc cagagaatat tcaccgaaag
     7741 tcttaaaaac ccttgcagat cttattgcgg agtctactgc ccatcaactt cttactctta
     7801 accaaagctg tgtttttcat ttactacaac ataaaccaag attacttcac gatattacag
     7861 aagaaaatgt tattgagtat atcaatcatc tggttcgact ggatactcat gatcaggaaa
     7921 tcatttatca gttaatggct ttgtttaggg tgttattgaa gaaaaaccat ccagcaacga
     7981 aagcagtatt cgaagcaatt ctcgataatc ttgtaaatca tccgcaactg ctggaggatg
     8041 aagaattttt aacacagtta aaaaaatatc cggattgtga gctcctttta gaggaacgtt
     8101 gtgaaaatat cctaaaacag cttaattttt gcattgctga acaaacatca ggactactct
     8161 ttggcaaaca taactacttc atcatagaag atgtttggct tggggcctta aggaaatttg
     8221 cagtactcca gcaaatcaac ccacagatga agtttagctt tgggcataag tatgcccttc
     8281 aagcaagaat tgccgaaatt gtctttattc atcagggaga cctgtttgat ttagatacct
     8341 ttatcgacgc gttggatttg ccccccgtca cctcatcagg agaaatcagt ccttacgaac
     8401 gggctcttat cgaaattctt gccacgatag ataatgatct catcagaaaa caaatcattc
     8461 aaaagctgga aacagcccct ttcaatagac ttgactggca tgaaagggaa tatggaaatc
     8521 aaacagtttt cattaaagca gcaaaaaagg gtaatttagg cttaatcaat ttgcttgaag
     8581 acaagataga accttctgtg cttaacaaag cgctaagaac agcagccaaa aactaccaat
     8641 gggaaacatt ggatcatctt tgtagtcttc ctgacgttga gttaaggcaa gatgaaatgg
     8701 atgatcttgt ggtctatctg gcagaacatg ggcgaataga aagtgtaaaa aaactcttga
     8761 aattatatga ttacaaacca tccacagaat taattggcac gattcttaaa aaagcgatag
     8821 acaatgataa tttacaagtc gtcatgtatt tctgtaaact gccagtgaaa tcacccaaac
     8881 aatcaatgct tgatcgccta tttaaattgg caatccaatt acagcattgg gacattgttg
     8941 agtatcttgc caactcaaag cattattctc catcccaaac gacgcttgaa aaggcttttc
     9001 aacaaactgc gctggctatg caacatgagg ctgtagaaat tcttggcaat gtggaaaaaa
     9061 ctcccgtacg tgcaatagtt attgagcggg ctttactgaa agccagtaag ctggggcata
     9121 caaaggttgt tcaatcgatt tgtgccttac cttccgaatc attaaccaaa caagctattg
     9181 aagacgctct agaacaagca gcggccgaag gacatttgga cattgtctcc tgcctctgtg
     9241 agtcaggaac cacaacgcta aggcaaccag ggatcaattc aggaatgaaa attgcggtcc
     9301 aggcaggcaa attatctgtg gttaattatt tctgctcgat gacagggagc aataaaccaa
     9361 ctccccgctt aattgatcag acattagtga tggcagctaa aaagggacaa acagccattt
     9421 ttatcgccat tcattcaaac caccagactc ctccgggaaa acatgctatt gagcaatcct
     9481 ttcaacttgc tatcactgca ggaaaattac caatcctaga ctatttgtgc cgtcatgaag
     9541 ggtatggtct caatcagagc aaggtagatc aagcattgat ttcagcggtc aaatccaaac
     9601 aacaggaaat tgttagctac ctatgtgaat cactagaaat tacacccagt cgaaaagtac
     9661 taagaattgc tgtaagtaaa gcaatttcca gtgaccagcc tggcttggcc gaatacttgc
     9721 gaagccgatc gatggacaaa tcaaaactca cagataccct cgttgatgag attgattcca
     9781 catccaaggt tggcaatcaa ttagaagcta acgggctatt taagaaaaag aaaaggcaaa
     9841 ccgacagaga gcctcagata ctttttaatc ttactattta aaactattgg tcagtagagg
     9901 ataatattct ttctttaatt tcgtttgacc ttgtgtacct cgcgattatt tttgcgcgaa
     9961 atatttttat cattagttgg tatatacaaa taataaccat atgagccggc agttactaag
    10021 ctatgtgcac tgccgttaaa tttgtctgac tcaataactg gttataaaga gaaaatcgcg
    10081 tgatcggaaa ccagatcagg caattgctca gccaattgca ggtgtaattt tgtttaaaac
    10141 cgtccataaa tttgcgacag tttgcatgaa atctccctca tcatacttcg atatccagat
    10201 caaacaatgt cagagaatcc atctcaccat cgtaaatttt cttaaaccaa tttggtattt
    10261 ttttcgttga ttcagaagca taacatgaac cttcttcata ctgtcctgga agatagctgt
    10321 taaataattt ttctaaagcg cggttatcat caattaacag atttatttgg ttaaccagat
    10381 aataagtttc tcgaggttta attttttgtc ggtggccata tttatcaagc catttcaatg
    10441 aagccaaacc aataaataaa gaccaggcac aaaataagcg atacgggcgt ccttcaataa
    10501 ttgggatgcc ctttatatag gccagtgaat ggtgtgcatg aataaccaaa ctctcgcgca
    10561 agctattctc tgaagaaata aaataacgcc cacttgtttc atcatccatt ttatctttca
    10621 agagattgat tttttgtaaa aagaaaccaa agtgaaaggc ttgatttaac aggctggctg
    10681 tccacttaaa attagatagt aaatcagtaa atatgtgcga caataatttg ccaaccaatc
    10741 ccgcaacaaa aaaacagtat tgattagtgg taactaatga aggaaatacg attttgcctt
    10801 ccatatgata ctgattaagg aaataatgca ttccatccac tacttgcaaa attgttttta
    10861 tcacagattg cttgatggat tcaggtaaat tatctttttc ttccaacaaa agagggacgg
    10921 cacatagaag ttgtttctcc cctaatggaa gctgcgaagg aaagctggaa agccattttg
    10981 tgaattgatc gtttgccggt gtttgaacta aaaatgattt aaaagctgca aatgaatcac
    11041 tttgcgtttg aagatcttgc catttggcat cttcaatact gtctgcaaca cgcaaaagta
    11101 aataactcaa cgcaacccat tcttttgctg gagaagttaa ctggcttata caaaaactaa
    11161 aacttctgct tacattttct agatgttggt aataaaaaga aaacgatcgc ataaccatca
    11221 gccaagttga tgttcgcttt tcgataatgc ccgttttatt ctctcgtaat tttcttccac
    11281 tgtttttgac aactcatcat atgaaatata atttttccat acgtgcatta aagaaaataa
    11341 atacagaaac ccaaactcca ggtcgttttc caataatgta ttactgtact tgataaagct
    11401 atctaaattt gaaaagggtt ttgacagtgg ctttatcctg gattgctcca tgtcttgaaa
    11461 catttgatca aaagcctcct gcatataacg cgggaacggg atattgccat caaaaactcg
    11521 gtgatcaaca ctcatgccta ctggcaaaac atcttgcacc tcaaatgtgt tatttgtttt
    11581 attccagact tgttttcttt cgatttctgt cagagttatt ttaaacgttt catttggaaa
    11641 taaaggtgaa accgctgctt gatatcccca atgaccaata ttgctcaaag aaatagcagg
    11701 tctggcaaat aagaaatcac ggtacactcg ttcgtgccgt ggagttagca agcgattgac
    11761 tacctcgatt aaatagggat gctctttttg taatgattgt gttttctcat aacagtacat
    11821 catgatgcac atgtcatgct gaatgtgttg cgctaattca gtcatagtca tctcatggca
    11881 gtttttaaac tcaatcgcac ctaattgatc actacctgga atctttaccg caagcgctac
    11941 gtaactattg tgcgagtggt acagtttatg attgaaaata taatttctcg tctgcggatt
    12001 atcaaaacaa gctttactca atgcttgtag catgaggcaa gttattgttt ccggtttaaa
    12061 tttcttttca aaaaaacaaa caggggtaac atttatatta aaaatagcag actcagttgg
    12121 taattcgggg tcattccaaa tcgctgaaag taatttacga atattggtcg gttcaatggc
    12181 tttgtctttt tcaaaatcat gcgataaatt cttcatcaat aaaggcatgg tatccataaa
    12241 caattgccaa tgggctgaag gcgttaagcc aattttttta agcatccaat ggtgtttatt
    12301 attgatgtta ttgagcagtt tcagtgcccc ttccttgcct aatagaatac caagggtagt
    12361 caaatattgc tcatgcgcaa atacggtatt tatcagctca tcttcaaaaa ttgctaattt
    12421 tttccttaaa tagtctttat ttggcaaccc aatctgacga tataaatcgt actcgtccat
    12481 atgccggctt tcatcttcaa ggactgttgc aaaaatagtt actgcgatgt ttttctcttt
    12541 cattgcaacg cataattctt ctacccatcc ttctccaacc aaattaacca aggtgataga
    12601 agtggccaga tcctcttcgc cttcaagaga agaaataaaa tgattgatac tcttttgatg
    12661 accaagtggt aatgcgtaag gagcggacaa ctgaaacgca acttttgcaa aaacagtggc
    12721 atggaaaatc tcgtcggtaa tttgttgacg catttgttga tacaaatctg ttccacggag
    12781 tttctctaga gattttataa gccctcttag gccaattgtt tctaattggg caaataagga
    12841 aaataaatga atatagataa gcttgtcatg ctcaagttgg aaaggaattt cgttgtaaaa
    12901 tatcttgtcc attcggcttt tgatggtttc cttttcctcg tcattcaata cagaccaagc
    12961 attatttaaa gaccgctccg cagcccaagt tggttttaac aaatcgccta actgaatcat
    13021 aaattaaccc caatccattt gttatattgc aaacgcctac actcttccga agcacatact
    13081 aatacttaag gggaagtgtg ctattgaaag cgttttttgt ggatattgtc atatactgat
    13141 tgtaattgct actggattcc agcctgaaaa taaccataga taaaaaaaga cttcttaaaa
    13201 ttatcttatc gatagcgata aaaccccttt gcgcaatgag ttattcacaa atctcgatag
    13261 tctgagatga ctagttctcg gctcactccg acttctcaac agggtagaca aagtacgacc
    13321 tgatatctca tgcaaaccaa agtacgttag ctgattgacg agtcatttaa aatcaaatct
    13381 ccatagtaaa ataagcatct cggaactgtt tgcaatgcag caatataaca aaagggacag
    13441 attagagaga agagctatta tctcaatgga aaataattga ttttaatagc aaaaatatct
    13501 ttgctattaa aaaaaaataa gttataacac tcttaactac atcaaggatt gattagtgca
    13561 aatgttgggt caacatcgcc gtaaattcat gttgagttga ctgtataatt ttcatggagt
    13621 gaaaaatgaa tagtaaagta ttttcgcaac gttttaatcg tgaattggca actctgggat
    13681 tccccgagga tttaacagaa aaaacgaaag ccgtatcaaa agtatttggt gtcacacgcc
    13741 atttggctaa tgcaatgata tttggccacg ttcttcctga taaagaacaa ctggataaaa
    13801 tcgcagaaat tctggaagtt tgtcctcaat ggctaagtgg tgcaacggat aggaaaaaag
    13861 cctactcagg ccgtgagact gctgatagtg tgtaataaac ctggaatttc atgtatactg
    13921 taagctaata gatagcgttt gctaaaatta tttactttgc aaacgctttt ttcattgttg
    13981 ggttattaaa atcatggaat gcttaagttt ctgtgtggcc aaaaccatag acttgactcg
    14041 tcttgatctt catctaaaaa acgtctctaa ggaatttagt gctgtaaaaa cccgagatgt
    14101 aataagatta aattctcata aaaacaaaga tcataccctg ttcattttta aaaatggaac
    14161 tgtcgtatct tggggtgtaa aaagatacca aatccacgaa tacctggata ttattaaact
    14221 gcttgtagac aaacctgtag cacttttggt gcatgatgaa tttcattatc aaattggtga
    14281 taaaacagcc atagaaccgc atggttttta tgatgtggat tgcctgacca ttgaagaaga
    14341 cagtgatgaa ctaaagctca gtttgtctta cggcttttct caatctgtaa aattacagta
    14401 tttcgaaacc attattgacg cattgattga aaaatataat ccgctgattc aggccttatc
    14461 tcataaagga gaaatgccta taagcagaaa acaaattcaa caagttattg gtgaaatttt
    14521 aggtgccaaa agcgagttga atttgattag taatttctta taccacccca aatatttttg
    14581 gcaacacccc actctagaag agcatttttc catgcttgag cgctatttac atatacaaag
    14641 acgcgtcaat gccattaacc atcgtcttga tacgcttaat gaaattttcg acatgttcaa
    14701 tggttatttg gaaagtcgcc atggccatca cttggaaata attattattg tattaatcgc
    14761 agtagaaatt ataattgcgg ttatgaattt ccatttttag ggtttttccc ttaatttatg
    14821 gccactctaa aatcctattt tctcaaagac acctctttgc gcacggaaat acttgccgga
    14881 gcaacgacct tccttaccat ggtttatatc gtttttgtca atccaaaggt tctgcacgat
    14941 gcaggaatgg atcaaggcgc ggtatttacc gccacttgtt tgataaccgc tttcgcctgc
    15001 gcattaacag gcttcatagc caacacaccg ataggtgtag cgccgagtgt ggctttaaat
    15061 atttatttct cttacagcgt agtacaaggg atgggagttg actggcaaca cgccttggcc
    15121 atggtattca tttccggttt gcttttcctg tttcttaccc tcaccaaatt aagacgcctc
    15181 ctggttgaag caatccctca taatttacaa atagccattg ttataggcat cagtttgcta
    15241 atcgccttaa ttgctttaga aaataatcaa atcattattg gtgaccagca caatttaatg
    15301 cgattagggc ctatcatcag accagaaaca ggactctttt tcctggggtt tattcttatt
    15361 cttactctgg attatcataa gattccaggt gccatcatca ttgggatact gagcatcagt
    15421 gtcttaagct tgatgttggg cttgaccagt tggcaaggtt tgattgctat gccgccgtcg
    15481 cttgaaccta cgtttttgca gctcgatttt tctggtcttt ccagtatcgt ggctttaaaa
    15541 accattttta ccttttttct gatcaccgtg tttgatgcga caggatcatt aattggctta
    15601 ctaagtccaa ccatttttaa acatgaaaaa aattattata aacgcctaac tcgcagttta
    15661 actgctgatg cagccgcatc ttccctcgca ggcctaatgg gctctgcgag tacctcacct
    15721 tatattgagt cagcaaccgg aattgaagcc ggtgggagaa ccgggctaac tgcgttagtc
    15781 attgcaactg gatttattct gatgctgttt ctttttccac ttgccaaaat gattccaagc
    15841 tttgcggttg gaccggcttt actctacgta gcgtgttgca tgataaaaca tattactaat
    15901 atgaaattac aggatatcac cgaaacagcg ccttgcatgg tcatcattat gatggtgcca
    15961 ttcaccgctt cgattgccga tggaataggt ttgggaatta tactttatac cctccttaaa
    16021 ttatttactc gtcaaaaacc caatcctttg attattgttt taagtcttgt ttttaccgtt
    16081 ttctttctga ttaactaagt gaatgtgatc tttaaatcct ggataaaccg aaaagcttcc
    16141 ataaaaaact tgtctactct caaaaactgc aaacaaaagt ttctttaatt tattgaattt
    16201 agtagtattt aaataagtga attatgcata agataggttg ttatcttgaa tcgttcgggc
    16261 taaggaggca tttatgccgt ctcgaagcct tatatggaat ttaatgtttc gtgccgaggc
    16321 ttcgagacga tgcttaggca tctcctcagc ccaaacgggc atcggatatc gactgcttct
    16381 tatataatac ttatccataa ctcacgttag atagccaaat aactgccata agcccatagt
    16441 tagcctaata gcgatgaaat attatgcttg aacaatcttt acgacattat tatcagcttg
    16501 tcctggtaga tcccatactg cctgctttac gtgacaggat aacgccattg tccattacct
    16561 ggttgtcggg aatatttggc ttgttgttta ttccttttgt cttattagac aaaccttata
    16621 ttgcaattag ctttctcttg ctttcgggat acctggatac tcttgatggt tcgctggccc
    16681 gctttcaaaa cagagcctct gactttggct cagcaatgga tatcgtcatg gatagaattg
    16741 tagaattttc tgctgttctt gctttgtatc tttttgatcc acaacaccgt gccactttag
    16801 ccattttaat gttaggcagt attttgctct gtattacaag ctttctggtt gtgggaattt
    16861 tttccaacaa tacctctcaa aaaagttttt attacagtcc cgggataatg gagcgtgcgg
    16921 aagcatttct attctttatt tttatgacat tattttccag ctgctttaat tttcttgccg
    16981 ctctcttttg ttttttagtc tgtctaaccg cgttgataag gctttttgaa tttaaaaaaa
    17041 gtgtacgggc tcctggttag cgaaacaata agttttccga agcctgattt aaaactttct
    17101 ctgattttat gctgactcca atacaaaatc ttgtataatt tacagcaatt cataaaagag
    17161 gcttacaatg ttgcgtatcc ttttgtattt tctcttactc attaacacaa cattttgttt
    17221 ggcaaagact gaaaaagcca tttttgcagg tggatgtttt tggtgcatgg aagcagactt
    17281 tgaccatctt cctggagtcc tgtccaccac atcaggattt gatggggaca ctcttaaaga
    17341 tccaacttat gaacaagttt ctgccggtaa tacccattac gccgaatcgg taatggtgga
    17401 gtttgatccc aataaattaa gttacaaaaa actggttgag tacttcttta gacatattga
    17461 tcctaccgtc aaaaatgcgc aattttgtga tcatggcagt caatatcgca gtgcaatttt
    17521 ttatcttagc gatcaacaaa agaaaattgc catggaatac aaaaaagagt tagagcaaaa
    17581 attccccaca gtttatacag aaattgcccg atcaacttct ttttatccgg cggaagaata
    17641 tcatcaaaat tattacaaaa aaaatcctat acgttataag tattatcgct atcgttgtgg
    17701 ccgggatacc cgaattcaag aggtttgggg taataaaaaa taaaagaata actcttaatc
    17761 atgagccata gttttatcgc ttatcaaaaa agaataagca attcatgaga aaacacgcta
    17821 atctaataaa taataatttt taatttttta ctaatacaat tatgattact caagttaacc
    17881 aattagatga acttcaatta aaagacttaa aaacattaag agcagagtgt aaaaaaaacg
    17941 atggtagcat ccccaatcta tatatccata tattgaaaca acaccgctct cttcctacaa
    18001 gcttccttta ttatcaaaac gggtccttga ttggattctt gagtgtttat ttcttttatg
    18061 atgatgctgt tgaagttgct gttctggtta gtccccaata tcgcagacaa gggattgcca
    18121 aacaattaat aaaggaagca ttacccttaa ttaagtctca aaactacttt aacttgattt
    18181 tttcatgccc ctcacgcctc aatgataact ggttaaccca aaaaggattt acctacctgc
    18241 atagcgaata tttcatggaa cgtgatgatt taaatcctat actcgattac atacgtccat
    18301 tgtcctttcg catggcgact ttagaggata ttcccatttt atgtggcctc gatgaagtat
    18361 gttttcctga taaaaaccaa gactcggtta accgattcca acaaattctt aacgaaagag
    18421 aatatgaaat tgtaatagcc atgctcaata atcatcccat aggaaaagcc catattcgct
    18481 ggcaaaccaa aagggctacc ttgtctgata ttgccatatt acctaaagaa caaggaaaag
    18541 gatttggttc tgccttgatt gctcattgca tcaatatgat tttgagcgaa ggcaaatccc
    18601 gggtggatct tgacgttgaa acccataata aaaaagcatt aaatctgtat attcagcttg
    18661 gatttcatat tcaaaatgct tgtgattact ggtccattaa tgtgaatcaa cttgcaaaat
    18721 aaaactgttt attcttttgt tacaaatcac atattataaa tatggcttga cataattaaa
    18781 atctcaccat tagtattgcg aatggcgata gaggaaggct tgttttatca aaaataagga
    18841 taattaatga aaaaaagggt tgttattact ggcatggaaa taacgtcatc cataggatcg
    18901 ggaattgata aattttggct tgctgcatct caaggtcaat gcggaattaa acgcatccaa
    18961 tcttatgatc catcccccta ttcgacccaa atagcaggtg agattacaga cttatctctt
    19021 gaccatctcc ccgaatttaa caaaagcaaa cgttatcccc gcgctgctca gtatgcatta
    19081 ttttgcgcac atcatgccat cgagaaagca gggcttgagc aagctgagct caatgcagca
    19141 ggcaccttta taggcacaag cattggcggc caacctgaac tggaagcagg ttacaggtta
    19201 ttctttacag acagctggaa gaaaatcccc cctcttagcg tgattagggg aatgcccaat
    19261 tcaatcgcca atcatatcgc aattgctttt ggcataggtg gaccaaactc aaccatttcc
    19321 aatgcctgtg tttcatccgg ggaagcgata ggcatggctt accaacaaat tgcccatggc
    19381 aatttgacca cagctctttg cggaggaaca gaatctttag tctgggaatc ggttatggct
    19441 gcctggtgta agcttagagt aatgtcaact aacaatgaaa atccctcaca agccagcaga
    19501 ccgtttgatc tcaatcggga tggaatggtt atggcggatg gggctgggat attaattctt
    19561 gaagagctac aacaagcaaa agcaagagga gcacaaattc tcggagaaat cattggctat
    19621 ggcgcaagtt gtgatgcatt ccatgtcaca gcgccaaact cacaaggaca agcaagagct
    19681 attgttgctg ctttaaatga tgccaaatta tcaattaacg atgtccatta catcaacgca
    19741 catgggacag gaacccagct caatgacagc acagaaacag aaacgattaa aatggttttc
    19801 ggtaaaagag cctatgacat cccgattact gctcaaaaag ccatgacagg gcatgcgatt
    19861 ggggcagcag gagcaatgga aatcattgca acaacactaa gcttgcaaaa aaatacgctg
    19921 ttaccgacta ttaacctgta caccccagac ccagcctgtg atctggatta tgtgcctaat
    19981 actgcgcgag aaaaaaccat tgatattgct ttatccaacc atttcgcttt cggtggagca
    20041 aatgccgctt tagttctgcg caaatacatt agttaagaat ataaatagct tacgctaaac
    20101 agcaattgat tattatacaa atgggtcaat cgtggccctt tggtaatatt aactccatca
    20161 ccacccaaat aacttttccc taagtctgca aactcatacc ctacgccaac ctgccagtta
    20221 cgatttaaca ttttttggac accggccccc acactgtaag caaaagcata agtggtgttg
    20281 gacgcaaacc atggtgcagg aaacaggaat tgattgatag aaagagtcct gtaatcatgg
    20341 gcattattaa atgctattcc aaaactacca ctcagataag gttgcacggg tgtaaatgca
    20401 cttcctatca gtttgccttt tagctctact cgtgcgtgat tcactttata gtcatacgta
    20461 tagacattaa ggaggccatt caggttgaca tcaccagtaa ccttggcatc gcttactccc
    20521 gctaccccta aacccaattg accaatcatt tggggattga tcgcacgctg cagcccaaag
    20581 aatatctcgc cattggctaa aatagtcgtt ggagaatcat gggtaaaata aagataataa
    20641 tttggcggta aattgggatt gaaattatta atatagactg gagacattgg ataaatatac
    20701 tgattttgtc caggtgttgc ccaagaaggt cctccgctca ctgtaataat agaggaccaa
    20761 agtaagggaa tttcttcgtc ataaatcggt tctgaagcgg aaagattcat actaaacaaa
    20821 acacataatc cggcgagcaa tatttttttc agcatatccc atccttttga ttacaaatca
    20881 gccctcttga ttcaggctct ttaatgaact gattttaata gaagttattt gattttatac
    20941 cctaattaat cttatttttc tagggtgcgt tgacaattca tctggatcac cagaatagca
    21001 ggatcatgat aagaaaaatt attaatctga aagccatctg aatcgaggat aattcactcg
    21061 aaaatggaca ataagaggta tataatcgct ctattttgcg gaattgaaag gtaaaccaat
    21121 gaattcttct cttcgtgata tagcaaacct tgttcaaggt atggttattg gcaatgatgc
    21181 catcactgtt tccacactat cacccataga taatatactt cccggatctt tagttttcgc
    21241 cgaaggtgaa gacaatatca aacttgctga aaactctgaa gcagcagcta ttcttattgg
    21301 tcaaggtact atcgattccc caaagccatt aattcaggta aaaaaccctt ttaaagcatt
    21361 tattgcctta ctcaaccatt tttatccccc aaggagaata tctccggggg tacaccccac
    21421 tgctgtaata ggtgcagaag tacaattagg tgatgaagtt tatgttgggc cattcgtagt
    21481 cattgaatca ggaagtatta taggcaacca ctcggtgctc aaaagccata ttcatatcgg
    21541 ccataatgtg gttattggag atcatacaac cattcacccc caagtcacta tttatgataa
    21601 ttgccgtatc ggttcgaatg tcactatcca tgcctcaaca gtaataggtt ctgatggctt
    21661 tgggtatact tttgttgatg gccaacatct caaagtacct cattcgggat atgtggtaat
    21721 tgaaaataat gtcgaaattg gagccaatac agcaatagat aaggccacat taggtgctac
    21781 agtgataggt gaaggtacaa aaattgataa tttggtacaa atcgctcact cagtcaaatt
    21841 aggcaagcat aatattattt gcgcttttac tggcattgcc ggcagtacca ctaccggaaa
    21901 caatgtcatc tttgcagcca acgtgggagt aagtgaccat gttcacatcg aggatgaggt
    21961 catattgggc gcacgtacag gagttccccc acacaaacac ttaaaaaaag ggacagtata
    22021 tcttggtaat ccggcaaaac ccaaagatgt tgcgattaag catgaactgt cagtgaatcg
    22081 tatcccatta atacgaaaaa acattaaatc acttaccgag caagtggctg taattaacaa
    22141 aaaactcgat attaaagcaa aagaggtaga gtaacgatga aaccgcctct gattctaata
    22201 gacggatctt cctatttctt tagagcgttt catgcccttc caccattgac aaactccaaa
    22261 ggccagccta caggcgccat ttatggagtt gccaatatga ttaaaaaaat aatcaaagat
    22321 tatcaaccag aagaaatagc cgttgtcttt gatgccaaag ggaaaacatt ccgagatgaa
    22381 tggtatccag aatacaaggc acacagacca ccaatgcctc aagaattgag ctctcagttt
    22441 caaccactaa ttcagttact gcaagcaatg ggattgccct tgcttatcat cgatggagtc
    22501 gaagcagatg atgtgattgg cactttggca aaacaagcca cagagcaagg cataccagtt
    22561 gttatatcaa ccggtgataa agacatggca caattggtca atgaacatgt ctccctcatc
    22621 aataccatga gcaattacag tatggatatt gatggtgtca aagcaaaatt tggtgtaaca
    22681 cctgagcaaa tcattgatta cctcactctc attggagaca gtgtagataa tattccggga
    22741 gtggaaaaat gcggcccaaa aacagctgta aaatggctga cagagtatca aacgcttgat
    22801 aatttgcttg aacacgccaa cgaaattggc ggaaaaattg gtgaatattt gcgtgccagc
    22861 attcctcatt tgccactctc aaaaaaattg gtcactatca aaactgattt ggatttgccc
    22921 ttaaatttaa atcaattgat gcccaaaccg gctgatagcg agcaattaat taaattaact
    22981 cgtgaattag aatttaagtc atggcttaaa gagttactac aggaagagga taatcaaaaa
    23041 tcacccaaag aaatggatgc cagcaataat aaatcctatg aaataataac gactcatcaa
    23101 cagttaaatc attggcttaa taagctagag caaagtcaac aattttgcat cgacacggaa
    23161 acaaccaacc ttgatgtcat gcaagctgag ttggtaggca tctctttagc agttgaggaa
    23221 gaaaatgcat cttatattcc ccttgctcat actgatggca gtacacagtt agtaaaagaa
    23281 gaggttttaa ctgctttaaa gcctattttg gaaaatccag ctataggaaa aatagggcaa
    23341 aacattaaat atgattacag tgtccttaaa aattatggca ttaccttaaa aggcattagg
    23401 tacgacacca tgctggaatc ctatgtgctt aatagtggtg caggcagaca tgatatggat
    23461 tctttggctt taaaatatct gggttataaa acaataagtt atgaggatgt tgcgggaaag
    23521 ggagccaaac agctccgttt tgaccaaatt ccagtagaga aagccgggat ctatgcagcc
    23581 gaagacgctg acattacttt gcgattacat cataagctgt accccatgct ggataagcca
    23641 ttgcgtaacg tgttgcatga tatcgaaatg cctttactca ctgttctcgc agacatggaa
    23701 atgcacggcg ttctcatcga tccagtcatt ctggaaaagc acggaagtcg tttgaaagaa
    23761 caaatgaaat ccctggaaca ggaagcaatt caactcgctg gaaaagcatt caatctcaac
    23821 tccccaaaac aattacaaga aatactattt gatgaacaaa aactgcctgt tgtagcgaaa
    23881 acccctacgg gacagccttc gactgcagaa tctgttttac aggaattggc ttttgattat
    23941 cgccttcctg ctgtaattct ggaataccga agcttatcaa aattggttag tacctatatt
    24001 gatgccttac ccaaaagaat caaccccaaa acacataggg tgcatacatc ctataatcaa
    24061 gcggtagcag caacaggccg attatcttcc agtgatccta acttacaaaa tatcccaata
    24121 cggagtgaag aaggaaggtt gattcgaact gccttcatag caccagaagg aagtcttatc
    24181 atggcggctg attactcgca aatcgagtta aggataatgg cgcatttatc tcaagatgat
    24241 aaccttttac gagcttttgc taatggttgg gacatccatg ccgcaacggc gagtgaaatc
    24301 tttcaaacga atttggatgc tgtgtccaaa gaacaacgaa ggcgtgccaa ggcagtgaac
    24361 tttggtttga tatatggtat gtctgccttt ggactggcaa aacaaattgg tgtggagcgt
    24421 caggatgctc aacattatat tgatacttat ttccgccgtt atcctaaggt acttgaatac
    24481 atggagcgta ctcgcaaaca agcgcatcaa cttggttatg ttgaaacctt gtttggccgt
    24541 cgtctctact tacctgaaat taattcacgt aacctaatga gacaaaaagc agctgagcgc
    24601 actgccatca acggaccaat gcaaggaacg gcggctgata ttataaaaaa aaccatgctg
    24661 gctgtttcca attgggagca ttcgcaaaag aacccctccg caaagatgat catgcaggta
    24721 catgatgaat tggtttttga agtccgaaag gaagcggttg aagaatgtcg cgccattatc
    24781 cacaagctca tggaacaaac tgtaaaatta tctgtgcctc ttgttgtgtc tattggcaca
    24841 ggttctaact gggatgatgc gcattgaagc gcataaaagc gtttaattta ttggatttaa
    24901 taataattaa tagaagtttg actcgcaaag gaaggatttt gaccacctat aaactcacca
    24961 aaataataag accacgtttt atgaatacgt tccaaaccaa tgactttggg ccaactatta
    25021 tactcattca aaaacaggcc atgactctta tacactttaa taaagtcttc tcgcatagca
    25081 tcggaatcag accaggctac aataggaagt tgcgggtatt tttgcaataa aattcctgca
    25141 agctcgggac cgttgcagtt catttcatca gggctggcac ccagatcacc atcaacaacg
    25201 acgatatcgg ggcaatagtt attgacgaag gataatgcgg cttgactatt attgacaatt
    25261 gtcactgaaa ttgatgcaac aaccgactcc aacaatcggc gcaaacaaaa ggcattaaaa
    25321 gcactgtcct caacaattaa tacacgcatc actgctccct cttaactacg agagaagtat
    25381 aatgtgcaaa ccttaagaaa atcttaagaa atagaattaa tccagttaaa aattacaaat
    25441 tgaaaaatca acgaagttcc ctattaaatt ttttttacaa attgtgattt taatttcatg
    25501 gcgcctatcc cgtcgatcct gcagtcaatg tcatgatcac cgtcaaccag acggatattt
    25561 tttactttag ttcctacctt tatcacttga gaagagcctt ttatttttaa atccttgacg
    25621 accgttatcg tatctccatt ttgcaacagg tttccattgg catctttgat aatttttgca
    25681 gactcttcgt cattgttatt actgtttttt ggccattcat gcgtacactc agggcaaatt
    25741 aatacgtcac cgtcctcata acagtattca gaaagacatt taggacaatt gggcaattgg
    25801 ttcattttgt tgaaaaaccc tgaattaagt tgattacaaa ctattttact ctaatagaaa
    25861 gcattttaaa aatttctgac tacattgatc tgtaatcaaa caactctaga atgaccggat
    25921 aaataatgat tttttaatca ttatttatcc aacgaaatag aaatgctatg aattgttgag
    25981 tggattagtc aatacgtatt cttcttggca ttgctcttct ttacctttcg tgggagttct
    26041 tcttgataga acgcttggga aatgaccaac cggatgaata ttgtttgtgc tcagcaattg
    26101 agaaccaccc aaactccgca tagtctcatg gctgctttgc ttaacagcag gaacagcctc
    26161 ttcgcttacg aactctgatg taatttctgt tccttcagca actgcaaatc caatagcact
    26221 tgatagtgca gcagcaccag taccacttat agcacctaaa gtcaaagctc ctaaaagacc
    26281 aacacctgaa ggagcaaaaa ctccggtagc aaccaatgta acacctaaag caaagccgaa
    26341 tccaaagcca atgcctgcaa aagcgcccat aatctttagg gtacgaaaat cagaagacgg
    26401 aaccggttgg ttagataaga cagtagccgt ttcttcacgc cgggataaat tatcctcttg
    26461 tggtaataca ggggcttctt caccgataga aagctcatct cctcgctcat ctggtaaact
    26521 aagctcttca gggggggtta ctttaggcgg gaacatttcc ctaacatctt ccaaatattg
    26581 ggaccattca aattccccag gattcttacg atacccacga atttcttcct cattgtatcc
    26641 cttacctttt acggcacgca gcatctcagc atagtctgtc gggctatcat caaggatata
    26701 aacccgatta gctttctctg tttggtgatt aagtcgggaa ttgacatcat taaatttata
    26761 ccctgactcc agcgtggtta acttgctgat ttcttcatca gaaaaacctt gatatttgaa
    26821 aactgcttta atgtattcaa ctcggttttt agtgacaata ttgacagtga catcatcatt
    26881 ttttaatact tcatggaaaa atgccaccgc ttctggtctc atcaggaaat caacatcttg
    26941 ctggctatga tcaaaatttt cactgtattc tccaaatttt gcttcgatca aagcctgaac
    27001 ttcaattgga tctttcattg gagtactttt atagtcctgt tccacatcct tttgatagcc
    27061 ttgcaacagt gactgataaa actcggtaaa tacagtttca ttaccgcttc ttccggttac
    27121 agtaccatca aaatcggtaa aaataataac tttaggcatt gtatttcctt actgtttctc
    27181 ttaatttgaa atgactcgat attgtacaaa gtttaaatta agagaatatt aagtatagaa
    27241 aactacacaa ttatcaggct atcttccctg tacaaattgc aatagacttt gcttatgagt
    27301 tttatcaaaa tgttttttgc atggaatact tataatttat agttgcaatt gattatttag
    27361 acagcatgaa gacctatgaa aaagcaccaa ccagtctggt tggcacactt cgttatagca
    27421 tatccagtat tttgcgtgat aaagatcgtg tacccttgca catgccgcgt catagaataa
    27481 ctttgctggg gatgaatagc actccccctc gtccgatccc ggtacctgat taatagaaaa
    27541 atcaaatgtc tatatcatgt gctaacaatc gcctattggc acggaaatag gtcattggag
    27601 aatgctcgag taaatcggat aatttttcca tgtagataca ggcgaatcta tcgacttggt
    27661 aggcgaagta gctctcttcc gccccagcgc gaaaaactct ttcccacttg ggattataga
    27721 aactgttttg ttcttgtaac aatcttgaaa tttgtaagtc aacagtggat atttgcaatt
    27781 gcaagtcatg aatttcctga tcatactgtt gagagctttc gtcaatactt cttgtgcaaa
    27841 gatcgacgta tttctgctct aattcttttt taatagtcat ggcctcaaca attttctttt
    27901 caattggcaa agcccttatt tgggatgcaa tttcttcacc taattcttca acaaccaatg
    27961 cagtacgcca attacaatct tttttcaatc gtaatatatc accgtaaata tgatccccta
    28021 tatagagaat ttcatctccg ccgaccccga gatcttcagt aaattttttg gcattacctc
    28081 cttggtatac accaggaact attgggccat ggacattggt catcgtcccg ttttcgggat
    28141 taactgataa aaatcgtaag ttatcataaa aaaaacgggg tttatttgcc aaggtgataa
    28201 caaactcaaa taaaccttgc cagtgctccc ctttatccag gaatggggac aaggcataat
    28261 ccaacaacaa tttagaataa gaatattctg aattggtcag aataaatatc tttttcccat
    28321 agcgaataaa atgcttcagc ccttcaacaa cttccttttc tcttatcaca tattttttca
    28381 aatttttaat aattatgttt ttaagagtac catccgagtg aactttatcc acacaatatt
    28441 gcacgtcttg tgctatggtt tgatagcttg gcattttatc gggattggta tcttttaaat
    28501 caaccaactg gccatacagg atgcaaaatg ctatagaaaa agaagtatca attgccatgt
    28561 aattcggatc acccaggtca acataaatac ttctgtaaat ttttttctga tcgctaaaac
    28621 tgatctgttt cgtaccatgg taactcaatc taatggcacc ataccggctc agttttaaaa
    28681 tattgccatt cttactgtca atcaccaagc cacgaatggc atcatcaaaa ttaaatttga
    28741 attttttaat ttcctcagga tagtgaaagc tttctgccag tctttctttg acgagatcat
    28801 aaactaagga ttcaaaattt ttcgaattat atcggatgag ggtatggtcc atgtccaaac
    28861 caatcagttt aatttttctc atatttataa ttcgatttac aaaaaccttg tgcgtatcca
    28921 tttttgagga acctataaaa ttattggcaa ttaatgtttt gagatatgaa cgtatacaag
    28981 tcggctattt taaattattg acaccacttg ctgaagccac taacacttta tctgccaaac
    29041 gtttggcaaa cactccattt tcaacgacac ccgggattac attaagactg tcttccaagg
    29101 ccataggtgt tgagaaattc agattaaaca cgtccagaat aatattacca ttatcagtaa
    29161 tgaagccttc tctgtattca gggtctcccc ctaatttaac aatttgtcgt gcaacaaaac
    29221 tacgggccat tggaatgact tccacagcaa cgggaaattc ccctaaacgt tttacaacct
    29281 tggattcatc gacaatacaa ataaattgtg tggctacatt cgctacaatt ttctcccgag
    29341 tcagagctcc tccacccccc tttatcattt caccgcgttc attgacttca tcagcgccat
    29401 caacatagat aggtaaatcc tgtactgaat ttaaatcaat tacagggata ccttctgccc
    29461 gaagcaatgc ctcggttgct tttgaactgg ctacacaagc ctctatctta tgtttgattg
    29521 ctgccagttc ttttataaaa aaattaacgg tagagcctgt tccaacacct ataaccatat
    29581 catcttcaat ataagctata gctgctttgg ctgctttgat ttttaactca ctcacgaaat
    29641 tgccctcttt gcctaattac ttcatacaat gaaacgccag tcgctactga cacatttaaa
    29701 ctctctacac ttcccatcat aggcaaagaa aacaatccat cgcaatgctc gcgagtcaat
    29761 cttcttaacc cggttccttc tgctcccatt acaagagcga tgtttccaga gcaatccatt
    29821 gcataaagta aatgctctgc ttcgccagct gctccaaaga tccagacacc ttgttgtttt
    29881 aagatgtcca tagctcttgc taaattagtt actctcacca aaggaattga ttcagcagcg
    29941 ccacaagcca ctttactgac cacgggagta atactcgcac tcttatcttt aggaatcaca
    30001 acaaaatcaa caccggttgc atcagccgac cgcaaacaag cgcctaaatt atggggatct
    30061 gtaattccat ccagaataag aatcagcaca ggctttttac ttctttctaa taaagtaacc
    30121 aaatcacttt caccgtattc gggtaatgcc gaagcgagtg caacaacgcc ctgatgcgta
    30181 aactctgaaa actgctgatt cattttttgc gcgcttagct tttcgatggg gacatgggcg
    30241 ttctctgcct tgatcaaaaa ctcatgaagt cttttatcta ttctatcttg attaacaaag
    30301 agttttttga tcattcgatg cgggtttgct aataatgcag aaactgcatg caacccgtaa
    30361 acatactgct cattcattac aaacttcatc ctcgacaggc tcaaaatcta ttttacgttc
    30421 gtttaaatca acacgagcta ccaacacagt cattttatct cccaaacgat aaacctgccc
    30481 gccgcgagcc cctattaaac gatgttttgc agagtcaaac gtataataat cgttttttaa
    30541 agaagtgaca tgaaccaagc cttctacata aatttcatca agctccacaa aaataccaaa
    30601 gctggtcaca gcggatattc gacccttaaa tacctgcccc aatttgtctt gcatatactc
    30661 gcactttaac caggtaatca cttcccgggt cgcctcatca gcacggcgct ccgtcatcga
    30721 ggaatgttta cccaaacgat tcatatcttc atggctatat tcaaactcat aaataggatg
    30781 attatccagt aaatgcccta ttgctctgtg aattaataga tcaggaaatc ttctgattgg
    30841 tgatgtaaaa tgcgtgtaag cggaataggc caaaccaaaa tgcccttcat tcgattcaac
    30901 atactgagct tgttttaaag aacgcaacat taccgtttca ataagatgct tctctggtcg
    30961 gtcaccaatc atgttcatgg tacgctgaaa atccttaggc ccaggttttc tcccccctcc
    31021 caatgacaat cccagctctc ctaaaaactg cctcaatgca gtaattttat cctcttcagg
    31081 agccgcatga actcggtata aagtaggaat ttcagctttt tctaaaaatc ttgctgttgc
    31141 aacattcgct gccagcatgc attcttcaat taatttatga gcgtcattac gtattacagg
    31201 cacgatacat tttattttct tattttcatc aaattcgata cgagtttccg tagtctcgaa
    31261 atccatagca cctctaagtc tacgggtatc gagcagaaca tgatataaat cataaagaga
    31321 ctcaagcata ggccacaagg cttgatgttt ttcatctgcc ttgccttgtt ccaaccatgc
    31381 cccaactgac gtgtaggtca aacgagcatg agagtgaatc actgcccgat aaaagcggga
    31441 tcgggatatt tttccctctt ggctaatcga taactcagcc accatacata agcggtcaac
    31501 atggggattt aacgagcaaa cgccattgga taaggcttcg ggtagcattg gcaccacttt
    31561 accgggaaaa taaactgaat ttcctcgcct tgctgcttct tgatccaaag cagaatcttt
    31621 ggccacataa tgactgacgt ctgcgatagc gacatacaat tggaagccgc ctttgggctt
    31681 tttgtaacaa tacacagcat catcaaaatc ttttgcatct tccccgtcaa tggttacaaa
    31741 aggaagatta cgcaaatcgg ttcgcccctt taattgctcc tcggaaatct gttgtggaat
    31801 tttacccact tccgcaagaa catcatctgg ccattcgaaa ggaatgccgt gagcatggat
    31861 tgctacctga atttccatac caggagccat atgctcaccc aaaatatgga ttactctgcc
    31921 tatagcttgc gaacgcttgt taggatatgc gattaactca accagtacta tttggccatt
    31981 tttggcctcg ccaatgaatt cttggggaat tgaaatatcc tgagtcagac gtttgctatc
    32041 aggaatgaca aaattcacgc catgatcagt aaaaaatcga cctacaaccg tggtattggc
    32101 atgttccagg acttcgtgga ttttagcttc tggccttcct cttctatcca atccagattg
    32161 ataggcaagc accagatctc catgcatgac agccctcatt tctttagcag agagcaccat
    32221 atcctcagaa ccatcatccg ggatgaaaaa tccaaaacca tcaggatggc cttgaaccgt
    32281 tccacgcaat aaattaattt tttgtaataa acaaaagcgt ccacgcctgt cctgcatgat
    32341 ttggccatca cgcagcatgg ccttcaagcg aaaacccaaa gattcctgct ggctctcaga
    32401 gctcaaatcc aatttatcaa acagtttact gcgagacatt ggcctaccgt attcatccag
    32461 gatttccatg atgtattccc ggcttggaat tggctcggcg tatttttcgc gttccctctt
    32521 ataaaaggga tctttggatt ttttactcac ttttcaccat ttttctcttc aaacctaatg
    32581 ctaactaaaa cctcttaagt cctgttctac catttatcac tataactagc aagaatatgc
    32641 tgccaactat cagagaattc tgtataaaac gccttttatt catctttttt ccaatcttac
    32701 aaggcaagaa gtcctcataa taatatgagg cttttttata ttgcatcatc tcaatccatt
    32761 tagcccatta ttaaagtttt aagttttcat aaggactcag gggtaggcag catggcagct
    32821 ggctaaacaa tttgccaaaa gctcaaaata gcctcatcag cagctatctt gaattgtaat
    32881 gagaattgat atgacttttt gcagcataga gtccatagcc atgccccaag atattcattg
    32941 ataaattatt tctccggcaa tacaaagcct cttccaccga ctgcggcatt taatgctgct
    33001 aatgacaggt cttccgctac ccagaaagta ccttgattgc ttgccggtat ttttactcct
    33061 ttccattgac atacggcaat agcattttca caaggtatct gttgctcatg ccctggcctt
    33121 gattctatgc aatttaaaat aaaggctggt ttgtccacta ccaaagctga ccatttgcca
    33181 gcttgcaatc ggcttgtcca acctgcactg gccccaggat ctgtaactgg atgagtaatc
    33241 catagatcca agttggtaat attatgaata aacaccaact tatttttctt ggcttttaaa
    33301 gttactgatt gaccttgtac aggaacagcc cgacaacctt caggaaaagt agattctgca
    33361 aaaagccctg ttgaaagcaa taaaagatag aaagagagca aaattctcat gattcacccc
    33421 atagtgttta tgtaaccaaa aattattaaa tcttcactaa taaatccatc atcaatatcg
    33481 gcatcgattt aagaaaacac tcatataata tccctactca tataagactt ccattgataa
    33541 gtcatgcatc gggttgatta atgcatgcag aacgcctggc taataaaaca aaaatatgag
    33601 caaaggtata aatgccctat aaggacaatc ctacattata ctttcaaagt tacttggtat
    33661 tgcaggagta ttatagtcaa tagactgttt gggttgttct ccaaagaatg tttgagtatt
    33721 gtctgaataa ttgacaactc gtgtgaaatg cgccggacta cttttaatag tagctaaaag
    33781 actaattaac tgcttgcgtc tttcagaaaa gcctaaatgc aaagaattgg ttctgctttc
    33841 tgcaaccggt gcattgatga aatcccctgc ttttttacga gtgggaccgg gagtggggtc
    33901 atcacaaaat tttataattt ttgatgagct gttttccgca ttaaaaccat aatgccataa
    33961 tcttttaaaa aatttaatta gcccaaaata aaatgtttgc acactggaac tcaagtcctg
    34021 ttccacttcc gcttctttgg cagcactaac caatcgtttt tttactcctt tatcttcaca
    34081 atccctggct aatagttcac atcctctttt tgctaaagaa taatgtttat tctgccccca
    34141 atacgttaat aaattaatat ccgcctgcgg gaaacttgtg tccttcctgg ccaactgttt
    34201 tccataataa aaggccatgt cacttaaaat ttgcctgtct aataactgag gtttttttat
    34261 catttcagct tccagcgcat cgtatatgac tgcggacaca ttgcgttgag ggaaaaatct
    34321 caataattga ctgactggcc tgatgtagtc tgacattcct tgaaaagaat taaaataatc
    34381 ctgtaataaa tgcaacaata cacttttctt acctgtataa ctcagcaaat aagaacatat
    34441 tatgggtgac ttcttctgtt tatctctttt actccaggtt tgcttaataa aatgactcca
    34501 gtccagcaga ggatgatggt cttttaattc cttttgcaag aagacttgat catcaaacca
    34561 ggagccctta aaaaacaaag attttccata aagttccctg attcctttag gcatccataa
    34621 tttatccacc atcctgacta aaaagggttc tgtgattgac aatcgggtta ttgcacgatt
    34681 caacgctttt tgcttgtaaa ataaagacgt tatccccgag ttggcgcacc tgaaaagaag
    34741 ctcgcctaaa acacgatatc caatatcatg tttattatcg aaaggacgtt ttttggataa
    34801 atgcctgctg gtaaaacagg ccagttcttt taattcttta ccttgccagg cctgaggaga
    34861 atcgggattc aaatcagttt cagaaaaaag gcgtccatgt tgattagcca aaatatgtaa
    34921 agccaattta tactgcgcat tttgctccca ataagaagtc ttatttaact gtgcaataag
    34981 gggcattata tgccgcctat caaaaaattg gcttactttg ccaatttccg gattatttac
    35041 cttctgaatg gagttatgag ttctatcacc aagaaactcc tttaacacta atgcggcaca
    35101 tgcttcagaa tttattaaac gatccagtac agcatcacag attgtctctt gcttcttatc
    35161 ttgagaaatt tccttggtaa tgaaagtcaa ggcgctactt agaagatctg gctttttata
    35221 ttttttacca tggtaagcaa atcgtagtaa gacttctgaa aaccagggtt tatcataata
    35281 attaaccaaa taatcagcca agcaaacatc ggcgccttga tgttccaaaa atctttgaaa
    35341 atcagattca tacaagccat tcagtaaaat agaacgcttt gggtcttggt ttaaaatata
    35401 atgaaaaagc tcaagcgcat tgtttttatt cgtgatagcg cctaataatt caaatactgt
    35461 ttcatcctca aaatgatcaa gcaatggata taaaacagat gcagggattt ctcctggagc
    35521 ctcttggatt tcttttaata atttattatt cagccatatg cgcttattgg catcaaggca
    35581 aatggattca cattgatata aaacccctat cgcgttctcg aataacacat tatgtaacag
    35641 ggactccaat ccaaacgagg aactttccaa atcacttttg gacaaaccag ctaaaaccca
    35701 ggcggcagca tttttgtgaa tggcactttc gcttcttaaa caactctctt ccgttaaata
    35761 ccctaaaacc aaaccctctt gatcatataa aggctcattg atatccatag agcccgttcg
    35821 agctaataat acgatttcct cattaacaaa agtcattgca gcattagact caccaagaaa
    35881 tccaatcttc ttcccggcag agttatataa aaaaatttct ttttttctgt ctaattcttt
    35941 gggcaactta tcaattagag gaaaactttg tcttaaccag tgaaataacc cgttccgaac
    36001 ttcggtttca ttattcccct taataaaaaa ccgtgaataa atatgattta ccactttaag
    36061 gaataaatcc gaacgtaaat tgggataact caaggcgtaa taatgatata acacctctaa
    36121 tcccttatta tgtagctcat ctcctccctt gttcgcctcc agtaaacact ccatttggtg
    36181 aagcaaagca cttttactaa tctcaactct aaactgcaat aaactgtatt ttttatccag
    36241 ccaaaatgac tgagcttccg gatgaataag cagattacgc tcaatctgag ccatatgttc
    36301 attgatttca tcaaaatatg gagcatattc tttaaaacaa aggtcttgct tatagtcctt
    36361 ctgactcaga gttaaggtat acaagctgta tctttcaact aattgtttta aaaccttagg
    36421 cgtacaaacc ccgacaaatt gtggcatgga taataataaa gcgattggag aactggataa
    36481 cggctccttt cctgagttac atgatttagt tattaaatcg gatgccagct gatatagctg
    36541 aggggtatca aagagataag gatccagctg attaaatttt gttcttattt cccccaaaaa
    36601 gtcctggctg gaatcagaaa aagccgaaca taacctggta agcaagtttt tacagttctg
    36661 cttctcttcc ccagtcgttt tgttatattc cgctatacaa tactcaatga tcaaaagcac
    36721 agtttcctga cttaagccct gaaaaaactc ttgttgtttg tctaatttgg tgttttttaa
    36781 taaatcatgc agcaactgca tgactaaatg gctttcagga gaaaataaat ctctttttaa
    36841 ttcatactga taaaaatcaa acacagcccg catggcgtgc aaatctatgg aaaaaggata
    36901 ctgagccagg caggctctag cctccttcag tttctcaatc gctttctcag aagaaaccaa
    36961 agacaacaac aatcgaattg agagagcatt gatagattca tcggttaatg aatcaaggaa
    37021 acgggttaac catcctgcca aatcaggttg acgtaaaaat tgtaccaaat ggtttctatt
    37081 gactaaatcc aaatccttat tgtttttgac taaaaaggtt tcaattaaaa catttaaatc
    37141 ggtatcttta gccagatgag aaaacgctga caaactgcgc tcatcggctt gctccaagat
    37201 ctgaattaaa tcctttgctc ctctttcttt caacgcgata aggacaggtt ttgcatcact
    37261 ataataattc aattgcttcg tgcatctctc tgctaattga ttatcaacac tggttttggt
    37321 ctcttttatt gaatctagca ttgtcagcaa ctgctctgat aactctcggt gaagcagatt
    37381 gggggcataa gtcaaaacga ttgcgagtac agatagcgaa gccggtaaac caaatggaac
    37441 tgagtgatta agcgagaatt gatttttctg agccgggatt tgttggcaaa agagttctgt
    37501 taaatcgtga gcatcttctt taaggtgact taacaacaaa tgttttaggt cgggaatggg
    37561 gagctgttga atttgtcctt ttattatact tattaactgc ttcattacga cacttcccta
    37621 acatcttaaa ataatccttt ccccaatagg gatttaaaag gataatgcct ttactatacc
    37681 cttaaactat tgatttgaaa actttaggat agcaaatgac gatctgggtt gatgccgatg
    37741 cttgcccgaa aatgataaaa gatatcctct ttcgcgcggc gatacgaacc aataccaacc
    37801 ttatcctggt agctaattcc taccttactt atccaaattc accatttatt cgctcaattc
    37861 ttgtagaaaa aggctatgac cgagcagatc attatattac gagtcatatg aaagctaaag
    37921 atttggtaat cacagcagat atccctttgg cagcagaagt aatagttaag caagggttag
    37981 caatgagccc aagaggagag ctgtttactg caaataatat caaacagcgt ctgactctta
    38041 gagatatcaa tgaacagttg cgttcagccg gagagcgcac aggaggtccc tctgcattaa
    38101 gcgcgaaaga aaaaaccagt tttgccaatg ccctggatcg ttggcttgca aaaaacaaat
    38161 aagacttcaa ttaaaacaca tattgacttt aatttgacaa aatcgattgc tcctcctaaa
    38221 ttttaagtac atcctaaatg gacttaaaaa catgaaacga ttcatgttca tccttttgat
    38281 ctttataaat ttccatgcct ttggaaaagc actcattatt ggcgttccag attttgctcc
    38341 accatttgta tcaagatctg gtggaggtga gttttatttc ggattcaaca tcgatttgat
    38401 gaactcgatt tgcaagatta ttgaggaaga atgccaatac aagggcatga acattcaaca
    38461 gctatatacc aatctcaaca caggaaaggt tgatattatg ctggcaccta ctccaatcgt
    38521 atccactgct ggtaccaatt atatcttcag tcttccttat ttgccgagca atgcccaaat
    38581 tgtaaccctg aaagctaatg accatatcaa tgccttaacg gatctaaaag gtaaaaaagt
    38641 tggtacttta aaatacactt tatacggtgg cctcctccag gagcattttc aggattatgt
    38701 tgatttgatg caattttcca aacttcccga tatggcaatc gcattgtcaa atcgagaaat
    38761 cgaagcagta gtacttaatg ccaatgcagc gaaatacgcg atgagcaatt ccgcgactga
    38821 gctcaaattt gtgggagatc ccattcctat aggtaatggc tatggaatac tagccctcaa
    38881 aaataatgcc ccgctcatca tgaaaatcaa caaggcttta ttacaaatag aaaaagacgg
    38941 aacctatttg gaaatatata atacctattt cggtgattaa gatattaccc taagcaattc
    39001 accaggcatt tcaaaaaccc aaaactagat ccaactccag aattcaggtt aaactaatac
    39061 caattgaatc tgtctctcat aatgcattga gcctcaatgc atgagatata gttcaaaaca
    39121 gtcgagttca agttatgaaa acaggcatat tttcatagac taaactcatc ttaaagtaca
    39181 atccaaatta atccgcagga tatgacatgc aagaaagttt tgatccatca actatcacta
    39241 cccttcataa aacgagcatc atacgctgga aagcgaatgg tttagagtat caacataaac
    39301 aattccttca acttgtagaa gaaaatcatg cttttaattt ccaattatgg catgcagaag
    39361 acaaggcgcg ccgagatgac atgggctttg aatttgttta tcaggccaaa agagaaatag
    39421 acgctttcaa tcagcaaaga aacaaccgca tggaagcaat ggatgagtgg ctttataaag
    39481 cattacaacc cgaaaaccat aaccattgcc cagtcaattc cgaatcacct gggatgatta
    39541 ttgacagatt atctatttta tcactgaaat catatcatat ggatttacaa accaaacgcc
    39601 aggatgtagg cgtagaacat agaatagcct gcacacaaaa actggaaatc atacagcaac
    39661 aattaaatca attggctttg tgtcttgatg aattacttaa tgaggtacga gctaaaacac
    39721 gtacgttcag agtttaccat caatttaaga tgtataacga tcctaagcta aacccagaat
    39781 tgtactgttc tgactaggta ttaattgatt taatgctgac tcgcttttcg aatgaggaat
    39841 tgatggagag tcgggttgat ttcttttggt tttggtttca aatataccga ataaagacag
    39901 agaatccgta tgccctgggg aatcagtccg agttttttca gattggattg attccctttg
    39961 ttcttcggtt ttctgcataa cttccttcgt tatctcaggg ctaaccgacg cttctttgct
    40021 gtcctccgtc agcttggcct gctgcgccaa gggagttcgt cccaatcctt tggctagagc
    40081 tcttaaagcc agagtaaccc caaacaataa ggcactgaag acagataaga cgtacataac
    40141 aggcgtatca cgataatggc catcctcgcc cagttcctgt aacgaattcc ccgcaaagtc
    40201 aataaatttt tgccccttac caaacccgga aaacaaggaa cgcaaaactt caaaccattc
    40261 ttgaaagaaa aattgtggtg aagggtctaa agacaatcca tcatttagta atttatgaaa
    40321 ttcttcagga gccaacagct cagtcttatt aaattcgtca ttaagacaat tattccactc
    40381 gatcaatcgc tcatatggac gttgtttttg agtttcctgc ttcgtctgat gccaataatt
    40441 actcaccaaa gaatgaatag tgaaactgag cactaatacc agacctaaaa taatacaagc
    40501 agtaagcaag gcaggaggga aagaaacacc tgccattact aaaatagaac ccactaaaaa
    40561 caagacactt gcaagcgcac cataagcata taatccattt ttcataccca acaaagcaga
    40621 cgataaataa gtgcggttcg attgttggga taataactta cgattatcgt caaaccgggt
    40681 tatcaaatta tggactaacc tttttagtcg tttgagctct tcctgatcaa ctttgtccaa
    40741 atccattttg tcttgcaata agagtaattt ggcgtaattg gtttccagct ccttggtaat
    40801 taaagccaat tgacatttgg tttgcgtcac taataatctc aactggaaat catattcctc
    40861 ataaatacgg gtaataacac aagccactgt ataaaaagca caaataagcg ccattgccat
    40921 aagcaactgt ggtgacaata cagccagact caatacacca acgtagagat ataaaccatc
    40981 aagaagtccg cctgcagcaa cagcccagaa tgcgaaatta cgagtttgca tggtctggta
    41041 tctgattcgc ttgaaataag aaaagctttc ctcatgagat aaagaaatta atttttttat
    41101 ttccatgaga agcgctatat tctcactcat cattgtttta cgatcttcca gcatgctacg
    41161 tatccaaaac ctgttggctg cagcaaatac ccccaaaatc actcccattg gaagaataag
    41221 aaattttgca tccactcctc caattaaact gaatgcaaca acagcgcttc tccagccttt
    41281 gtaagcattt ttcaaaccct tcatgacatc acgaaaatat ggccaggcat tagcaataaa
    41341 ttttttatag taatcttctt tttcattctc aaattgacag gccaaaacag aaaaggtaac
    41401 taaaaatact gactcaactg caatggtaat aagaccggct ggagaattta agaattcatg
    41461 catcgcatcg ttatcattag gagatgcaaa cagatcaaaa aaatatttga acatgctgta
    41521 cgaggaactt agagagtcca taacagcgta ggcataatag atactgcgac ttttatcgag
    41581 tttttgggcg aggattctac tttgcgcgtc gatacgacca gcaattgcta aaatgaagac
    41641 taaatcagta ggcttggaag cctgattttc atcactcgca tgaggcatgt ataggtatgg
    41701 ggctatcagc taagattggg cgctattgta tgcataatta gacttgatgt caattaaact
    41761 ttcatcctga gttaaaaaaa tacaacaatg acctcaaacc cttcataata acacatccca
    41821 ttagcaaaat tattaaaaaa aataaaaaaa attgcattaa attctctctc agactgtgac
    41881 ttatgcctta aacctgctac gatattgcac caaaataaaa cccaatatga ggattaaatg
    41941 agatcaaaat ttgttctctt tatgttaatg tcactactga caggatctct tatggcaaac
    42001 agtgaagctc aaatctatgg ctacgtagag aaagccacat taatcgatca aaacctcact
    42061 ttatctgcaa agcttgatac tggcgctaaa tcagcatcat tacatgccgt gaatatcaca
    42121 gaaattgaaa aaaaaggcat tccttattta cgttttaccg ttccaaccaa aaccggtgat
    42181 tactcatttg aaggagagta tgtcggcaaa gtaaagatta aagtaaggtc aagtgaagcc
    42241 aatccaggat tattaagaac aactcccatt aaacgaccgg tagtcttact taatatcaag
    42301 cttggtgata aagtcagaac aattaaagtg aacttaacta acaggaaacg attcctttac
    42361 cctttattgt tgggaagaga tgccattatt gattttaatg gggccgttga tcctgccctt
    42421 actttcacaa ctaaatccaa gagcccagtg aacaatgaaa aataacaaac gtcatgttta
    42481 tggcttgatc ctcactctat tgcttttagg atcaggtatt tttttatata gacatatcat
    42541 tctcgatgtc cctctgaccg atacggaaac gatcaacagt tggatggttg aatccaatct
    42601 acgttttacc gctgatagaa atacgccgat aaaggcaagc tttaatatcc cttacctgcc
    42661 ccccaatttt gccattctcg atgaatattt tgtgtccaga aattatggtg tcaccaccaa
    42721 tttaaacggt tccaatagag aaaccgtatg gtctataagg cgtggtcatg gtcctcaatc
    42781 actttattat agagcaatct tccgccaaac agacagtgat gagtcttctt tgcctaaacc
    42841 ttcggtaact aaatcacaac ctctgaatga cagtcaaaaa tcggccgttg aaacgattac
    42901 caatcaagtg agatcaacat cagcagacat tcaaacattt gcccaaagca ctattaaaga
    42961 attaaacaaa agagatggta atgcgaagct tttagttggg aacgaattca atgatgacaa
    43021 tattatcaat gccaccattt tgattctcaa tcaatcaaag attccggcca ttgcagtcca
    43081 ggggatttat ctaaaccaac aaaaaaaggc cgatttaaaa tccctgctcg ctgtgtttaa
    43141 tggaaaaaac tggatttaca ttaaccccaa aactggcagt gccggcttgc caaaagagtt
    43201 tctgatttgg caatatggga atggccctct atttaatgtt gttgggggta atagagctca
    43261 attttcatta actgtatcgc caacacccat taatgcctta agtgtggcca aatcaagagg
    43321 gctagaagac tcgcaacttt tgcgcttctc cctactccag ctacctgtca atgtacaagg
    43381 gatctataaa atcttgctaa ctgttcctat tggagcattt atcattttaa tactgcgtaa
    43441 ttttataggc attaaaactt ttggtacgtt tatgcccgtc ttgatcgccc tggcctttag
    43501 agagactcat gtagcatggg gcattacttt atttgttatt attatttctt tcgggctatt
    43561 agcccgattt tatctggatc aattaagact gctgttagtt ccaagacttg cagccattct
    43621 cactgtagtg attctattaa tgatctttat cagcgttcta tgccaaaatc ttagtcttga
    43681 caccggtatg tctgtcgcat tattccctat ggtcatctta accatgacta tcgagcgcat
    43741 gtgcatcaca tgggatgaaa gaggggcttc cgaagcaata aaatcaggga ttggcagtct
    43801 tgctgcagca gttatttcat atggggcgat gagttatgag cccttacaat atttgatctt
    43861 tgcctttccc gaattacttt tggtgctttt gtccttgatt ttatggttcg gccaatatcg
    43921 tggctatcgt ttagtcgaat taaaacgctt taaatcactt gcgagtgcga tgaaatgatt
    43981 aacctgttta ggcgcctgaa aaatcatggg gttttaagca ttaaccagcg gaatacggat
    44041 tttgtattac gatacaatcc aagaaagtta tttcctttgg ttgatgacaa gctcaaaacc
    44101 aaaaaactgg ccttaaaagc tggaattgca gttcctgcgt tatatgacat cattgaaact
    44161 gagcaacaaa tcaaatcaat agaaacccga tttgccagct ataatgattt cgtggtaaaa
    44221 ccggcgagag gttctggtgg cgatggaata ctcgtattta aagataaagt ttacggtcga
    44281 tatcgacaaa ttaatggtaa gctgactacc gtccaggaac tgagttacca tttatcttgc
    44341 ctgttatccg gggcttatag ccttggcggg tcctcagatt atgccatcat tgaaaaacga
    44401 gtggtcgttg acccggtatt tgcggaggtc agttacgaag gcatacccga tatacgtatt
    44461 attaccctat taggctatcc ggcaatggcc atggtaagac tgcccacccg tttatccggc
    44521 ggaaaagcca acttacatca aggagcaata ggcgtaggtg ttgatcttgc aacaggaaaa
    44581 actcttggcg gcgtgtttca aaatgatact atagactacc atcccgacac gttaaatccc
    44641 atcgtaggca tcgaagtacc ttattggaat cagattcttg aaatagcctc cagttgctat
    44701 gagttaactg gtttgggtta tcttggagtt gatattgtac ttgataaaga acatggccca
    44761 ttaatgctgg aattaaatgc cagaccagga ttaaatatac aaatagccaa ccgagagggc
    44821 gggcttaagc gctatagaac aattgaagcc cgttttaagg aaaatggcaa agaatctact
    44881 ctggacaaag ttaatttttg ccgcaaagag tttgcacgct aaagtgtcgg taacgcagaa
    44941 caatttagtt ctttatccag ggataaatta ttccactctt ttaaaaatcg gcgacgagca
    45001 catagccaat cgatcatttc atccatctct acacgctctt ttttagattg aatttttgcc
    45061 agatttcctt gcatattact ccaaaacaat ccttttacat catgcccgtt agcacatcgc
    45121 cttagtgcgg gattcaagca ttcttcatca attttttttg tcagctcttc tctctgtctt
    45181 agcagttgct ctaaaatcag tttttcagca tggatattct cttgaaatcg cttcaatcca
    45241 actcgatgaa gctcctgctt aaactcttgc tttatggatt caaaggtagt ttctgcaggc
    45301 attcgtccta aaaccatctt tgataaatca agcgttggta agcatgcggg gcggcgcaac
    45361 cataatcgtc caaacatgaa ctctccattc aaatcatcaa caaatttcat tattgccaaa
    45421 caaatacatt taacaaataa atagactatc aatttttaaa gcacaagttt caatttaatt
    45481 cataatgaaa tataataaaa caacgaatgg atttaattgt taattgcata gaaaatccct
    45541 cctttctgca tatgaaaacg agagaatttt acttgtttaa aatccatctt taatgcaaat
    45601 cattaacaga acaataccat actgacgctc tggcatgcca tattcgttgg ggagattttt
    45661 tatgcggtct aaacttgttt catttttctc acgctcgtca agaccgatga aagctatccc
    45721 ctctaaaatt tggtttgaga cacaattaaa aagcagcgga cttgacaaaa aattttccat
    45781 agatgagttg agagaaacgc aatcttccgt cagagtattt gctaataaga aatatcttcc
    45841 agatactcaa acgattaatg aggcattaac taaagtaaca gccatcaatg tgtcaggaga
    45901 taagagcggg tattttcaaa atggattacc gttcccaaat gaagcaggat attttgagaa
    45961 aatccctgca ggacatcctg aattattgtc tcccatagaa aggttagcaa gtagtaagaa
    46021 agttgtttct tcgcacagcc tggtcaccgc atcaggaggc tatcccttga ctaatccatt
    46081 attaccctat cgaaaaccaa ttaaagtaag tattttttct ttagcaggcc cctcttttga
    46141 aaataattac ctccattatc gtttatttct attagatcct atacaaaaga taattgattc
    46201 accgctattt agccatttgc atgatggttt acccattcac tttgatgatg cgaaaaagga
    46261 gttaggtggg tatgatacca acaaagtgat ggctcgcatt cgccttggtt ttccttatct
    46321 ggcaaggttt tcatcaggtg gtttttatcc cagctttagc aagtcaaacg ccattatttt
    46381 cttatctgaa gcctattttc gctatcagct agaagatatt tcattactac tggcgtcagt
    46441 aaatcaaacc gcaaaagaaa ctggaaaagc agccttcctc aaagccacag cagttgggat
    46501 gggttttttt gcaaaaattg attgcggtta tgatatacaa catatgattt ttccttatta
    46561 tttacgagcc tataaaaaac tgttaagtga acataaattt ccatggatag caaaaattga
    46621 atttcctatt tttaatgaaa ttcaacaaga acagtttgat tcaatttttg aagactataa
    46681 tggctctacc aaagtgtatc aaagcacacg cgatgttttg gagtttcgag aagacgagat
    46741 tgaaaaatac ttccctgctg cgattaatcc ttcagatgcg tttgctctca caggaaatga
    46801 gtggggatat ggtagcgttg aatccatgat tggaaataat agttcgatcc gctttgacca
    46861 agtacatcat atgaatcctc ttattttaga tccatctcat catgttgcag tacaaataaa
    46921 taaggatcat cgcgttgaat tgtcagccaa tgccatgcca caactctcgt cgagcattaa
    46981 accctagggg atgatgcagc ttccttcaca taataagtaa cagcatctgc aagatattta
    47041 tccccctcaa atcgcatcaa caaaatttta agaggcgtat ctccgtatat ttctttgcaa
    47101 agcttggcaa actcttcttc acaatcatta atcaataacc attctaaagt gcttttttcc
    47161 gagctgaaat caccgagtct catagtgtgt gggactttgg atttaaaatc cttcatagca
    47221 ttgggaacta atcctccacc aaggtatttt tccctatatt ttgggcaagt agatgcaata
    47281 atttgttcaa atgatacgct atcgacaacc agttcttctt tttcctcatc atgatcacca
    47341 catgatattc ctacttcaga ctttaaggta agaagagaag ttgaacttcc ataaaattta
    47401 tgaacccatg cttgtttagt caattcggaa taattctcca ggaattttcg caaatcctct
    47461 gcagaagcac aagcgcccca tccaaatgga ttcatattaa atttctggta tctttccaca
    47521 tcggtgatac gaactttttc cagcttctcg ctttcaacac gttgtggcaa accacctaat
    47581 ttgtgtaaat ggtctaagct acgtccgtct gctcgaacca atattttttt agtctgtaat
    47641 tccttgattt tcgagcccac cagaggatta ctcaaatttg agacactggt ttccaactga
    47701 agtaatgctt tttgaaactt ggaaaaaaat tttaatctgg tcattaccaa acctctaaag
    47761 aatagtaggc aacctaaaaa acttcagaaa taagagtaag tgatttaaaa cctcggagag
    47821 ttttttaggt tagcaaaaaa attatacata acaattatta actaaaaatt aagcattata
    47881 tattttttga aaaaaagcct tactttggcg aaaaattact tataaaccgc tagcctggcg
    47941 cataaataat cgtttaattg ggataaattc attgcatgcc cgcaatccga gactgcgtaa
    48001 tgaggtaaca ggagatgcgg aggttccaaa taatctttta aatccctcca taagcaaaat
    48061 aatttgccaa acagaatgct tgcgttccct ttggtatgcc cctaatactt ttttggatat
    48121 caattcacct ttacccattt caagacaggt taaccagctt ttcacatcag ccaggccaac
    48181 atttaaccca agaccggcta atggatgaat agtgtgggct gcatcaccaa gcaataacca
    48241 gcgatgccct gcatattgct tgacatgtct catctgtaaa ggaaattgat ggcgactgct
    48301 tactaccttt atttgcccta gctgacctcc aaatgcctta gttaattccc ggctaaattc
    48361 attatcatca agtaatttca atcgcatcgc attatcggca tgagttgacc aaacgataga
    48421 acattgattg agattagata atggcaaaaa agccaaaggg ccatcaggat gaaacacttg
    48481 aaaagcagta ttctgatgtg atttctcact atttaccgtt gcgaccaatg cttgctgctc
    48541 ataagaccag ctggttacat ccaccttaag cttatgacgc ataggggagt tagctccatc
    48601 agcgatcatc aataactgcc cttcccaggc attattttgg ccggagatct tgatgctccg
    48661 ctctaaatgg cacacctcat ctattacact gtgaggaaat aaacttattt tggattctga
    48721 tgagattcga cgcaacagag cttgctttaa gattgactct tcaataattg cccctaaatt
    48781 tggctcagca atactgcgag aatcaaagtc aatatatgcg ccacttacac catcccagac
    48841 atgcatttta ttataagggg cgacccgggc atcatcgagg tgctgccaca ctcctaattc
    48901 acttaataac atttttgatg ccttattgat ggcataaaca cgcaaatcac tcttgtctgt
    48961 attgaccatc aatgtccctg catcgataac agcaacagta taatggcgtt gggccatagc
    49021 taaagctgct gtcaagccaa caatcccacc tccaatgaca agtacatcaa aatgttggct
    49081 cattgaaatt ctcctgcact taatgcaatt tcacaaacca aatcaggagt aattccgcca
    49141 aaaccccgtg catatcgcgc taataaattt tttaatgccg gtatattatc aagtgccagc
    49201 aatccaaggt tacgcgctac tcccataccg ggtaaacgac tcgtaaatag atgtatcaaa
    49261 ccgtctgtta gccaggtaat catttgctga tcatgtgctc gcaatttcaa ataatgctcc
    49321 aacatctgtg gatttaatcc cttttggctg atgcattgtg ccaacgccgc aatatctctt
    49381 aaacccaagt taaacccctg acccgcaact ggatgcaaag tgtgagcggc attaccaaca
    49441 aaaacaacag gccatttggt ttgctggggc attaaaactt gtttcagggg ataagagtat
    49501 cgccttccta ctttaccaaa tcgtcctaac ctatacccaa aagcagtttg cagttctcgc
    49561 aaaaaatcac catcattcaa catcactaac cgttttgctt cttcaggctt tgatgcccat
    49621 accaatgaca ttttgtcatt catcataggc aataatgcaa gaggaccatt aggtgtaaat
    49681 ctctcaaaag cacgattacc atgaggtttt atcaggccca cattggcgac aatagcgtct
    49741 tgagcataat ctttgatttt gactggcaga ttacagaggt ggcgagtcat cgattccgtc
    49801 ccatcggccg cgacaattaa ttgagcccgg atagaaatca tatcgtcgcc atgggcgaca
    49861 gtagcagtag aattgttaaa gtcgatggat tttaaaaccg caggagctaa caagtgttct
    49921 ttattgatta ggatactcaa agccctgttg atgtattgca tttctaccac atagcccaaa
    49981 gggctatttt tttcgccttg caaacgagaa accccaaagc gatgctgatc agaaacatga
    50041 atcatatgaa ttggtgttgt atattctttg agaatctccc aaactcccag cattgccaat
    50101 atacgttgac ttgcaggcga caacgccaat gagcgagcat caaaatcagg atttacctta
    50161 tcgctgtatg gcctggattc tactaacgtg acagagaaac ctaatccctg cagagccagg
    50221 gataagcttg caccagtcaa accaccacca ataatcagta tatcagtctc tttattcgcc
    50281 acgcatcaaa gcctcaatat catctacatt aacaggcaag tcaccggtca atacctcata
    50341 tcctgccctg gttaccagaa tatcatcttc tatacgtaca ccaatatccc accatcgttt
    50401 atcaacgcct tccatccctg cgctgatata cagccctggt tctactgtta ataccattcc
    50461 agcctctaaa ggacgccatt cattattgat tttatatcgc ccgacatcat gcacatccaa
    50521 tcctaaccaa tgtcctgaat tatgcatata aaatggttta taggcttctc ttttaatgag
    50581 ctcctccaaa tcgcccttta aaattcctat atcacagaga ccctcagtaa gagttctgat
    50641 aataatctgc tgaatatgat tccagggaag gcccggttca atcgctgcta tgccagcctt
    50701 ttgtgctttc aataccaatt cataaatgat tttctgctca gggctaaaag taccgcttac
    50761 cgggaaagtt ctggtaatgt ctgcggcata actaccatat tcaccacctg catcaatgag
    50821 caccaaatcg ccacggcgca aaggtttgtt attttctgta taatgaagaa tgcaggcatt
    50881 ggcgccacca cctacgatag ggtcataagc tacactacga cagccatgac ggctaaactc
    50941 atagattaat tctgcctcca gttggtactc atattccatt ttctgacaaa cttgcatcgc
    51001 tcgtttatga gcagccactg aaatacgggc tgcctgtctc attaactcta attccgcatc
    51061 gcttttaagc aaccgcattt cgcttagaat aggctcgaga tcgcataagg actcaggtgc
    51121 cttaatgccc cttctcacct gggcttttag tgaagataag acatgcctga tttttttttc
    51181 caataataaa ttacggccga ggtcataata tatggcagat ttcccgctga atagtttagg
    51241 aagctcttct gtaatactgc ttaatgggta tgccgcctgg actccaagct cttgcaaagc
    51301 gccgtcctga cctaatcgtt tacctgtcca ttgttcttct gcaggatttc ttgggcgatt
    51361 aaacaaaata ctttggctat cctttcccgc aataatgacc agcaaagcat caggctcatt
    51421 aaatcctgtt aaataataaa aatcactgtc ctgtcgaaat cgataatgag tatctccact
    51481 gcgcagcact tcatgtgctg caggtattac agcaatactg tcatcaggca attgcgcagc
    51541 taattttttt cttctcgcct tatattcttg ttggcttatc atatcaattt cccttttcac
    51601 tttaataaat agaacaaatg agcaatcctt aaaaaatcaa agaaaccaat aaatacaaac
    51661 aacttcgata ctttatcaac ttgctatcaa tgggttgttc cagaatctcc aagttctctt
    51721 tcgtgcaaaa ccaaatcact gtgcaatcga agtactgcca tacgagtgta ttcactgacc
    51781 tccatcaacg ctctctcatc ttcctcgcct acctccaaag tctcacagtc cagctctgca
    51841 aattccataa ggtgctgaag agcatcctga gattcctctt cataaaactg ctccatccca
    51901 actcctgcaa tagttaaccc ttgcgtaaat ccttcacacc actcactaaa agcctgagca
    51961 cgagtgacta aagattcatc gtcatcgggc aataacattt caaattcaaa atcaaaatta
    52021 ttcatttgtt gctggctaat tgaaaagaca gaaaacatgg ataataacgc attacggctt
    52081 tgctcatctt ttttattatt tagcaaagca cgtatgtaag cctcgccttg actatcagcc
    52141 cctgcacaca gataaccgca catgattccg tgtaattcgc ttccggacat agttaatgct
    52201 aaaacagaaa tggattccac aaaatcatcg tacttaggta aatgcagcga atcattatct
    52261 aaagacatat ttaaccctat ttaaaaagat agctaatgat actccattta gaatcagtgt
    52321 tcacctgctg cactttctca acacctatga ttttcataat ttgctgcatt aaggcaaaat
    52381 tgctactatt tgcataatac cgtgcaaata caaaggcg
//
LOCUS       DBNCWK010000007        52090 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.007, whole genome shotgun sequence.
ACCESSION   DBNCWK010000007 DBNCWK010000000
VERSION     DBNCWK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 52090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52090
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.007"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1273
                     /gene="dctA"
                     /locus_tag="AC06IO_000347"
     CDS             <1..1273
                     /gene="dctA"
                     /locus_tag="AC06IO_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HIO9873565.1"
                     /translation="YVQVLLGVFIGILLGYFLPDVGVQLKAIADVFIKIIKMLISPII
                     FLTLVSGIAAMNDMRQVGRLAGGALLFFTFITTLALLIGLLATDYFKPGVGLNVNPNS
                     LDAKEASSYFEGAHKVTNVTDLLINIIPRTFISAFVDGELLQVIFISILFAIGLILTG
                     NAGKSLVTGMQTLSNVFFKIIHFVMYMAPVAAFAAMAFSVGKFGVGPLFNLIGLLACY
                     YLTSIFFILIILGSLLQWYCKINIWHLLRYLKDELLIVWSTASSETVLPSLMQKLERL
                     GCEQSVVGLILPLGYSFNLAGTAIYLTMAAMFIAQATNTDLTVWQELSLLGVMIISSK
                     GAAGVSGSGFIVLASSLATIGHIPAAGVVLVLGIDKFMNEARSIINMMANAIATIIIS
                     RWQQSLDYQKTRFELQEKGQAETKSVYASEM"
     gene            complement(1537..4437)
                     /locus_tag="AC06IO_000348"
     CDS             complement(1537..4437)
                     /locus_tag="AC06IO_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873566.1"
                     /translation="MLELNDKETAVIKAIHHNHKKRLESQMITRFGVYFEQSKHPEKL
                     YSWIEKIEDTRLLANHYLDTIALSRAPSTISSLEKETAGSSYYIEKAKLLIPESIRNF
                     LSDINSSNDNTLFNFVAAEVFSLALGKSITDLLFDYFNNEFLGAPENVQKEFNETLEK
                     QTELSNLIKKECERISIEIIKLFHFREMLLLGKISKSPIEPNSREEFIAKAKIANQSG
                     ESVHLAIEIYFARELSRIFNSGFKSLYQVHDVEIQSEHQSPLVKWINQMSESEQVRLA
                     FTQEIQLKFMLLARDFLLEKMNQKGILARYPIIFSLFAGVIIAPIVGVTMIIMTGGTF
                     LWAIAALAVMVFALSCIATHLLINKLSVLTYQRSSDNRAHIQQSIDMINGEFLRLKKE
                     SLLKKETTSEVIENTRDFQKINQNFHLVTIGKKVARGSVSGWLREYASRYRHSKAIEI
                     DLGDEYKELITQSRIQTQELIHSINKKNSKLLIKWIHDTMTYLQDEKNQSVIEEFELI
                     PKMKEQVLEIVCQLNYIPPMLLKFYSNSREKGGLGGSESDFSHIKRLTPIDAPDKSRH
                     NPYNYLCDTALNLFKKYEPLYTSTCIFLGDPEYRQMLGIARRDAREPLTAENINRYLD
                     NSYAFLLSLCHKIKPGLGLDPLQQSARVTDEFVLYRMLLLKQLAAISESDSKEISNEI
                     KFKIRLFIQKYFNQDTKVIFDDLENQMFLLNKEAPNSRIYKNSDHFEVIDTELDNIFQ
                     AITLDMAYNSNNFRFVDILDYFINRFIKEHDSQSATVFAYGKAEQEINPQGTKYYLKI
                     LAQYCNNTTEFLKSAQTNTTLTATNLLDCYRYNISLQVCRTQIRIICSIKEINKLKMT
                     NETTEQITLLLKSFQQLAEFAKIHCFLLKTDSSCYRLFKLIENKAMQSTLSRDWIRQL
                     DAKEALIYMMDDLPNQLKLTADYNASSLFFSSAKQAKREKMENSEEPIMKPA"
     gene            4466..4684
                     /locus_tag="AC06IO_000349"
     CDS             4466..4684
                     /locus_tag="AC06IO_000349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873567.1"
                     /translation="MTLCNKGYAKRLYLLLLVSWHFPAKILQNLDTSHLLFPHKHSEG
                     VHRKIGGNFSACPCQKVKAVITERARQS"
     gene            complement(4744..5388)
                     /gene="upp"
                     /locus_tag="AC06IO_000350"
     CDS             complement(4744..5388)
                     /gene="upp"
                     /locus_tag="AC06IO_000350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIO9873568.1"
                     /translation="MDFNQVKVINHPLIQHKLTIMRKKETSTVKFRTLMHEVSMLLAY
                     EVTRDLEIEYEEIETPLATMQSPVLKGKKLVFVSILRAGNGLLDGMLQLVPTARIGHI
                     GLYRDPKTLEAVEYYFKLPEHTQDRDVIVVDPMLATGNSAIAAVKEVKALHPKSIKFL
                     CLLAAPEGISNFHGEHPDVPIFTAAIDEQLNDHGYIVPGLGDAGDRLYGTKLAH"
     gene            complement(5401..6642)
                     /locus_tag="AC06IO_000351"
     CDS             complement(5401..6642)
                     /locus_tag="AC06IO_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HIO9873569.1"
                     /translation="MNNQEQQIQNVLNTLRDPRTIRTRSHRILNLAKQNRLEHFSLNP
                     ERMTTTASYIVDIITSRYPDLDIPYHSRWRHFEMDGLPRIHNLRKQLEPISPSEWGKI
                     LYELVIISVFLDAGAGPLWRYKEALTEKEYSRSEGLALASLYLYESGAFSADPTNPFR
                     VDAERLLDFKETELIKGFQVSSTNLLEGIPGRVSLLNRLGEIIHRNEYCFNRRKRLGE
                     FYTYVESLQDNKMLPTTKLFEAVLEAFNDIWPVRLIYHGVSLGDVWIHSALKTNEAGS
                     EYIPFHKLSQWLTYSLVEPLEQTGIDVTDLDVLTGLPEYRNGGLLIDTELLKVRNNKI
                     LEQPQDPGSEPIVEWRALTVALLDELALLIRQQINMSAESLPLAKILQGGTWEAGRQI
                     AQKKRINGTPPIEIISDGTIF"
     gene            complement(6635..7903)
                     /locus_tag="AC06IO_000352"
     CDS             complement(6635..7903)
                     /locus_tag="AC06IO_000352"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIO9873570.1"
                     /translation="MSPDDSKKKSKGHIVLSSHPASHTQAPLKIKWGEKNPKERGPVI
                     ASLTNIKNRNAVGTHSGSYSVYRALAVAAGLLDPEHVPDLTDTLPPVDIGPHKQWGEA
                     GKIVSLDPWGHLVASVFSEEIKAGYDIRPTIAVTRAHINIPELHIAMQKGRLKPDGKI
                     LSETGDVVVTKAAIEPVWYLPGIAERFQVSEALLRRTLFEQTAGMFPELVTRSDLDVF
                     LPPIGGLTAYFFGDVTTIHNPEIELTCRIHDECNGSDVFGSDICTCRPYLVHGIELSI
                     ESAQKGGAGLIVYNRKEGRALGEVTKFLVYNARKRQKGGDTAAKYFERTECVAGVQDM
                     RFQELMSDILHWLGITKIHRFVSMSNMKYDALLKSGINVIERVKIPDELIPPDARVEI
                     DAKRAAGYYSPEGIIDINELAKPKGRALHE"
     gene            complement(7990..9198)
                     /locus_tag="AC06IO_000353"
     CDS             complement(7990..9198)
                     /locus_tag="AC06IO_000353"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIO9873571.1"
                     /translation="MSDISRGDIRVKGFTDSEMDFQLIRQLGSSSYKAASVGECLNIA
                     NTMKNEDPEEWVRQFERLAEWQKKDGLERMVKNHVISGREQLFKASNSFRAAEYYSPP
                     SSEHHRKLGLNSADCFCTAISSMDIHFENHSIPYKNINLPCYFISPANDGEKRDTVMI
                     VSGFDGTLEEEFLMRGLAAVERGYNVIHFAGPGQMDVFRKYPDTYFEPDFEQVVKRII
                     NYFEFRQEINMAKLTLMGISFGGYFATRAASHEPRIKVLIANSPILDLHAYMCSFVKM
                     DPCEMPDSEDFSINDLPSIPNEVMPPQLKAQTEHLIRRFGQPTFKATFNYLREFTVGD
                     SLNHIKIPCLALLGSGEGGEPGKQFAEFCKITNSAQYEFSDFEGAGSHCQVGNVSYSN
                     AVVYDWLDSL"
     gene            complement(9426..13283)
                     /locus_tag="AC06IO_000354"
     CDS             complement(9426..13283)
                     /locus_tag="AC06IO_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2239 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873572.1"
                     /translation="MKMNEYIELYERHRYEGNNTIDALILTLNSLKQSRESWGQDNKL
                     AKYLGFGLRNAKHPFFQPKVTQKNLHVIETVIEKLNALKESPDYQQEGETSTTRLDIL
                     GILGERFNELSKNGKTRELFNYVMFNEGSIDFSMRESTLVEPSPLEILTGNEEPYEDE
                     QTWEPPYIHQLLDSKGIPYTIFDSLETNDLNLENDLGDHVRWAYDNIYRYSYPDSHQE
                     STIARFHHGIQHVTRAANYACAFANLYRRHGNEEAQNLTETDIKLIQIALLFHDSARE
                     GDDEDLWDHESAIFLYYYLTRVLHIDAETARFVAEATANKDPSPENGYFQLIEDEYGQ
                     LFWQFAKYEEGDFPAKNIYQKIIHDCDCLDIIRARNQYDATYLDFYKDIASKDNLALE
                     EMAELVVEARGLIYSQGDSYITKKAEIKAGYENEEAHARIIRDMQSEAYPILSTLYGK
                     LLSIEELQAMTLVNLTPFDETAGMAGENLAAALREGRVLARGITTPSAMPTPRPNKPT
                     LTDETLAEREMRKSMRSDNINIKSRKEAPNLKYKNPLRSTSILGYGSGVYPAAGMLIF
                     NPDIGQIKRISTEDFDSGRGQKNHLAHLQEKEYKESREILERELDDLHHKMKLGLFGK
                     KTQLGSNYVELLYDINKYDAIFFTNDPTIGNEIAYNDFNPAHPLSPLLQAIYLQKQYA
                     RQYEETRKAYYERYGQERGETLFLERFGPNQFLPIYEYSGLRNKLTPYDTEKLTDENI
                     VNMWVEMSSDFMRNALSHMGRDNSYDFYDELLYNMSIEDIKVLSMYKTKGNILAKVNQ
                     PADLSYNVELREKITDAIAKAKEKIIAEYEEAVFKRLQDNQLSPFSKEAFLALEYSPR
                     LRDLCSDKIKDDLLRFIYSSDGVISYKLSDIASTSLKEITTTLDIQHCSTDERNFLCK
                     NQFLQAFILCQQLGLEGEIHFLRNKVNELAIQVINDQNAFITGSKYELQSGLHQMINL
                     NNIMTLIDKDNHLRDNIESILLEIVSKLIDTYTNERLNLYDSYMLLKTLQNAGIPDVK
                     LKHYFRPVLNKANETLHERKPHDWGHYCSIAKLVGDEAKPYIYSWLNDYDKPFYSEIN
                     SMLETFKNAGIVFDDENLDVFKSLLEKIHYNDVAYNYVRLSYWLRVMNNLQSLASESH
                     YSESQLEILKNQFDRVIEKLIKTTKSEYEHASYYSQEQKCYRQLTSFAEEINTILASD
                     FCLPVPDSLITEFNLTLIAFSEMDLSQTKVDESKFHLLKQLHNKLPNPEKREHAMEKL
                     TESMDTKKQVEKDQNQNISAAGL"
     gene            13494..13880
                     /locus_tag="AC06IO_000355"
     CDS             13494..13880
                     /locus_tag="AC06IO_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIO9873573.1"
                     /translation="MYTIIVCLFIAILLPYLAKVPVAYAMHKAGGYDNRYPREQQARL
                     QGFGARALAAHQNAFESLLIFATASLTALATNSVTLMTQYFAIAYIISRLFYHLFYLL
                     NCSSLRSTVWFLGVICCLSILWLSIP"
     gene            14042..15874
                     /locus_tag="AC06IO_000356"
     CDS             14042..15874
                     /locus_tag="AC06IO_000356"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9873574.1"
                     /translation="MSDLQQLPKTILRFSWHFIKKSPWLYALFFIAPVVMVLETNVIP
                     YALKMIIDGITNHKGGKESIFQDIAPALYLGGVAWFGVILVLRLHNWWQAYLVPQFQA
                     NIRMTLFGYLIQHSHHFFSNQLAGNLANKISDIPRSLEAIRKIVSCNVNTTFAAIVVS
                     IVLMLTINPWFAFILLSWIIIQLFISFYSAKEINKLARVNAEDKSILKGKIVDSLNNI
                     NTVKLFVQNEYEKKYVWKTQNKEVQSNKRLVLFINFFRLLVDVPVSIMLIAMIYSVIS
                     FWQRNLISTGDVVFIFATSFAIMNQMWSLSNSLCDLFVEIGIVKQALAILSQPIEVQD
                     IANAKELKVTHGEIRFENVTFRHSDGQELFTNKSLVIPAKQKIGLVGYSGGGKTTFIN
                     LILRYFDVDSGRILIDNQDISEITQYSLRKSISMIPQDTNLFHRTLLENIQYGKEDAD
                     RDEVVTAASLANCMDFIQNLPQGFNTIVGERGTKLSGGQRQRIAIARAFLHHTPILIL
                     DEATSQLDSLTEECIQESLQPLCEGKTTIVVAHRLSTLLNMDRILVFDKGTIVEDGTH
                     QELIENNGLYKSMWEAQTSGFLPVEQEDEAELAKEFDRPYLAQV"
     gene            complement(16065..18407)
                     /locus_tag="AC06IO_000357"
     CDS             complement(16065..18407)
                     /locus_tag="AC06IO_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HIO9873575.1"
                     /translation="MHTIANETPVQKDHSFDPITNVLKWVLLVTAILCFLILIWGTVK
                     TYQLVPPLPDRFVTSTGETVMTSQDIVEGKAGFQRADLMDYGSLYGMGSYFGEDYTAK
                     YLVRLGELVQEELAKIRFNKAYSELTESEQFVINKEMQNSLQRLDLNKKTSILTPAIA
                     TSIRQLQAEISQSLLNHNFMAGYTQAYSLNPDTALQTANFLIYSSLTTIARRPNQNIS
                     YTNNWPYEPSVGNTPSSGTFYWTWISFCFVFMGFGAVIYIYHRYLSDVDHGPMEPIFS
                     GFKPMTQSQRKVGQYFLVAALFLLLQIAVGAIMAHYYAERESFYGINVNTWLPFNFLR
                     DVHIQTPIIWIGLSWISAGIFMAPIISGKEAKGQGFLVDFLFYVTLLVVGGAILGNYL
                     GIMGYIDKAWFWLGNQGLSYIQLGRLWQIGFCIGLFLWSFIVFRGMWPTWDGLKKATI
                     EFWTGRIKLENLFWASTINVAVLYCFGMIPLTGIEKSFTITDFWRWWVVHLWVEQSFE
                     FFAACATAYLLMGTGLVSRKAAERTVYFESILIFLGGVIGTGHHWYWTGTPEMWIPLG
                     SMFSFIEVLPLVLLIIDAIEHRQLIKKQGTFTYNLAYLYILGAAFWNFVGAGVFGGGT
                     LNAPLINYYEHGTFLTLNHAHTALFGAFGLLALGLIYFCLRYAAGESKAWSDRLGVYA
                     FWLYNVGLFLWIVLNFFPIGWSQLMAVYEHGLSYARSLEFYNTTLLWQWLRFPGDVVF
                     ALGALLMAYDFIVKIGPFFPKLARNRRFIAGPPKATDPSL"
     gene            complement(18553..19755)
                     /locus_tag="AC06IO_000358"
     CDS             complement(18553..19755)
                     /locus_tag="AC06IO_000358"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIO9873576.1"
                     /translation="MDINLMVFASPLFLILIGIETWVAYYKKSGVYQLNDSVNNFSSG
                     ILEEIGALPIKGLIIYSYYYLYEHYAIFNVNTQSIFSWLLLWIGVDFFYYWYHRVSHR
                     NSFFWIGHSVHHQSEHFNLSVALRQGYFQTLTSWVFYLPLALLGFPTWMFVIVASVNT
                     IYQFWIHTESIRKIGWFEKIFNTPSHHRVHHGKNPQYIDKNYAGSLIIWDKLFGTFEP
                     EEAPADYGVTEPLDSWNPFYANIKVVKDILYYGKGLKSKWDVLRAFFMPPEWIIHRLQ
                     QEKSPVSKKTTIKKNTTSPGIYMLTNTAFAVLLYTYLSLVFDPAMITSWLIGALILLT
                     LYTLGRIADGHSNTLYLEISRATLVLLILLLFKNTLLLSLGCSVLFYVINYALCTTFP
                     YKRTASSF"
     gene            19909..21132
                     /locus_tag="AC06IO_000359"
     CDS             19909..21132
                     /locus_tag="AC06IO_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIO9873577.1"
                     /translation="MHQEHPGQTDTPKIDPWLIFVILMLMALMQTTTDQYIPSLPAIT
                     TALNSTEASIQLTISMFMLGLSLSHIFYGPLSDKIGRKPPLMFGVGLSILGSLVCFFA
                     PNVSVLILGRFLQGFGIGCCNSVGRSLVRDLFTDRLLAKIGSYVGVVSIFIMAASPTL
                     GGFIQEMAGWKANFMFLFVFGILVWLLAFSILPETNKHLNPEATKIKVMAKNYFTLLR
                     SKIFVGYTLCACFAFAGMVSYLTIAPFLFQNTMGLSPLEFGRLTFFIAGAICFSGIVN
                     SQLVMRKGISFMVCTGVLFMIAGGLSLLLITMLGWSQIYFIMISMTLFSAGAGFTFIN
                     AFAGAFHPFPQMAGTVGALYASMQDLSAALTSGFIAGIKGYGQYSLAMILLILGLSSL
                     VAWYYLGLQDDAISN"
     gene            21142..21369
                     /locus_tag="AC06IO_000360"
     CDS             21142..21369
                     /locus_tag="AC06IO_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIO9873578.1"
                     /translation="MKITDEELKKIISKLSGSKADDIHDDTALIEDLHLDSLKIVELL
                     AILSEEYQLEVSEEDAMNFHTYKDLYDFTQA"
     gene            complement(21689..22699)
                     /locus_tag="AC06IO_000361"
     CDS             complement(21689..22699)
                     /locus_tag="AC06IO_000361"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIO9873579.1"
                     /translation="MNFFRCEKPIYIKGPFVALPERVMSNQDVLNWMNSTQNPAVIGF
                     STGIKNRHWVNEDQACSDLAVRAAEHLFMEKPREKHKVNQVILATISGDYPSPPSSPL
                     VQYRLGLQNAGAFDIGAACAGFVVGLHTSAALAQTNDGSVLLIASEIRSKFLNKNNFA
                     TSVLFGDGAAACCVSQDKEEADFRFIASALFADGEVYDAVSTPAGGSRLPAAVCNDSE
                     QFYITIKESTALFVKAVHGMADSAKDFLKELNLTISDIQWLVPHQGNKNLVLSVAKQL
                     GFPEEKTIKTVEETGNTSGSSVGIALDRLRSDGKIKSGEKVLLVAAGGGGIAACSLLE
                     VI"
     gene            complement(22696..23448)
                     /locus_tag="AC06IO_000362"
     CDS             complement(22696..23448)
                     /locus_tag="AC06IO_000362"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9873580.1"
                     /translation="MNILITGGSSDIAQAIAKRRSESGDKIIITCSNNDSLNETLAIY
                     RQQNIDVTGFIYNFSDPQACENDLELILREPLDGIILNAFTRVTQLRKLHALPYHAVR
                     TYLDNNLNGNIWLIHRLLPALLKNKFGRLVLISSLSATAGTSRYPVYCTAKAALEGLF
                     LNLAVDYSANNILSNIVRVGLIKTSRTEQFWKNPAYQEKVIQIIPQGTMGEPSQVAEA
                     IDPLLSKTSFMTGSVVTVSGGLPLMRSEGLLS"
     gene            complement(23445..23678)
                     /locus_tag="AC06IO_000363"
     CDS             complement(23445..23678)
                     /locus_tag="AC06IO_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIO9873581.1"
                     /translation="MLSNRIEQVLERIGLTNLPENKQAKLEQGGLDSLMLALLIIELE
                     REFEIKIPVIPLEKEHYESINTIEQYLIELGAK"
     gene            complement(23668..25047)
                     /locus_tag="AC06IO_000364"
     CDS             complement(23668..25047)
                     /locus_tag="AC06IO_000364"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIO9873582.1"
                     /translation="MLFLASDVPNKVAITQGNRVITFDELTHEVLASSKQFLKLPKTI
                     IVLHATPEIGFIIQLLACLETGRPIALFPNSISAEEKQRRLSLLGNAVMIDEEGELQE
                     ICENKTIQPHPQTALILFTSGSTGKVKAVQLSSINIKANCHAVIKALEFKKIQDQLLF
                     LPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQSTVPQMWSGVPSHWVAINAI
                     AKRYPDSAAKINAIVSAGAPLSTALRTDLKQIFPNAIIYNNYGLTEASPRVLTYSSDD
                     PLFTENYAGYPVGDWKIKLSAEHELLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEI
                     LPNGLVAIKGRRDSLVNIGGEKVNLSEIEQKICQIDVIKEAIVIPQEDKIYGTRLIIC
                     IEKKSLPSSISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGL
                     DNKEKNNAK"
     gene            complement(25052..26797)
                     /locus_tag="AC06IO_000365"
     CDS             complement(25052..26797)
                     /locus_tag="AC06IO_000365"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HIO9873583.1"
                     /translation="MKKEYLQCQSLVDVVRLRALHSPKKRSCTFLNKELEEIMTYEQL
                     DQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLL
                     DKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPT
                     SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHD
                     MGLIGCILTPIYGGIQAIMMSPFSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRI
                     REEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKEFGFRKEAFYPCYGLAEATLLV
                     TGGTPGSPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPC
                     DFDQVGEIWVQSNSVAKGYWNQPEETEHAFSGKIKENENSPIYLRTGDLGFLHENELY
                     VTGRIKDLIIIYGKNHYPQDIEFSLMHSPLHHVLGKCAAFVIQEEHEYKLTVMCEVKN
                     RFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCRKHLLDK
                     TLPIVATWQLNKIEE"
     gene            complement(26809..27870)
                     /locus_tag="AC06IO_000366"
     CDS             complement(26809..27870)
                     /locus_tag="AC06IO_000366"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIO9873584.1"
                     /translation="MNILKPNIQFEITGASRILPASGPITNFEILKNFPRTADKSQGF
                     LEKFAEKIGEEFGFHTRYWCHKPWESLDNSRELSSESLAIQAVEKLIAHYKPEDIDAF
                     LLGSTTNKRFTGSQAAAVLGSLGLNAPAYDLKTGCSTSLSTLHFAYALMALGYQKILV
                     CCSETLSKVIDPENEKTWIGLADGAASLLLEKNKEGSFTIEKSFFSTDGQYVDAFTTQ
                     GVFPPTHKQLDSVGYHLVGDETLMKELAYSRYMQMLDNLLPTEKEKNEITWIIPHQVN
                     RKLIDQVLHENHLNNKIIIWDADTIGNIGGASILYTLARAVEEKLFDRSGKILLMSVG
                     GGLSYAGQVLNYQKASFSN"
     gene            28097..29518
                     /locus_tag="AC06IO_000367"
     CDS             28097..29518
                     /locus_tag="AC06IO_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIO9873585.1"
                     /translation="MSSQHTDKTLIKWIDNLIDEQSFLPIGSEYIPDNKPHSLPGAEV
                     ITGLAKVNQRPVAIYAHDSSVNRGYVTRQGARKIIHLMDKAEKLRIPIVAFLASPGVS
                     LEENLLSGDEYTQIIARNIRLSGIIPQFAVILAPTLGAPAYSAVLMDLLFFNKHRSYL
                     MVTSPTVVQQAIGEKVTMGELGSATLHATTTGIADFVDDSVTAQINHVKAILGFFPLH
                     TNERPPVHPIMEPNHPIPDIPANPRIPFNMLNLIQAVVDNSNVIQYKRTYGQAMICAF
                     VHIHGFAVGVVANQSIRYSGAIDSDAAQKAAKFIRICDAYSIPILTFIDVPGFMPGKR
                     EEQKGLLQHGARFCVAMQTRVPRLSVIVRKCYGAAAFLMMQTKSQNGDLVLALETANL
                     GVMGKETTNKVQAMDGQPVQESSQSQLTEKSQIDDSLMEAYSLGLIDEIIQAAQIRSR
                     LASHLEFLYRRMETMPPAKHSIV"
     gene            29527..31218
                     /locus_tag="AC06IO_000368"
     CDS             29527..31218
                     /locus_tag="AC06IO_000368"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIO9873586.1"
                     /translation="MTSYENLIQLSEFFESCLGNPLEDNSPVNFQQILLADEQERLAS
                     SPIEFIKQWGYLDYTVPRYLGGKLSSLDELYTLTRLLARRDITMAIMFGLAFFAALPI
                     WIAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNARPTEDGWEISGRKWCV
                     NFATLSEYAVVLCRTNEKGGLLGFSLFYIDKKSAEKGISPTPKLPTHGVRGLDISGFS
                     FDKVKVSKDALIGKEQRGLEITYKVFQISRTLCACLAIGGADTALRLALSYSLQRQLY
                     GKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPEKMSLWSAIIKFLIPHIGE
                     DIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGM
                     RGKHWAEHSEKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITT
                     IRHEIEKLDQEVIRLHDEKLFDPRSLAVFRLAEKYCWIFAASCCLQFWHYNQEILSDD
                     LQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIHIAG"
     gene            31225..32754
                     /locus_tag="AC06IO_000369"
     CDS             31225..32754
                     /locus_tag="AC06IO_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 family domain-containing protein"
                     /protein_id="HIO9873587.1"
                     /translation="MNWKRFLIAAMTKKKYQVFLNDTKHPKEARERLWNSEIAPLLEK
                     SSYWQKLLKDQSPVVLNDFGITEYEDYQEGLLAAQHSTIQPFNGEELIFWSETSGTSG
                     VRKFFPITDSFQKQFQRTMAPYIYSLTQRFPGFFKEKMLYLVAVDTHKTTPAGIPTGW
                     ISNFNYRHLPSFIKRFYAMPDEVFASHEIYEQWGPVYGLASDLSAVFAVTPMVIDAFY
                     KRCIDGFKDYLPHLLDDKPLPSHLPPLKMTRERRRHLQALAKADNLSFKQLWPSLEMA
                     GCWTSGLCEYPAQQLQKLLGPEIKLVDGTYSATEGWLTVPIETNSVGGILHPGAHIVE
                     FIPEGAAIEKENLLQCWELEVGKKYEVFLTTAMGFIRYRLKDIVKCTGYLNSSPKLEF
                     CYKTQLLKLETCSITGKELQSAVLATHLDIEPYWYFARNSVGNRIVLVTDDEAEISEL
                     SLKKLHEQLIHISPTYAHSVETEEVLPVTLLQLPKEKLLAGMHGQTKPKFISQEVIAE
                     R"
     gene            32772..33370
                     /locus_tag="AC06IO_000370"
                     /pseudo
     CDS             32772..33370
                     /locus_tag="AC06IO_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947932.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2223 family Dot/Icm T4SS effector"
     gene            33626..34753
                     /gene="lpnE"
                     /locus_tag="AC06IO_000371"
     CDS             33626..34753
                     /gene="lpnE"
                     /locus_tag="AC06IO_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LpnE"
                     /protein_id="HIO9873588.1"
                     /translation="MDMKKYILSLSLLTVINSSAIATTQEGEEAYNKGYYEKAFILLS
                     PEADKGDAKAQYLLGKMYYNAQGVTYNPEKTEQLLLASANQGNVDAQVLLAGFYWYLN
                     TPEGYKKAFEWYQKAADQNNADGQYGLGYMYDTGTGVPQNSDTAMVWYKKAAEQGNSN
                     AALAIGYNYDTGTGVKKDKTQALNWYAKAADLGNASAQYNLGLMYEQGDGVPKDFQKA
                     AEYFEKAANQDHAKSQLELGYLYDSGKLGKSDLQKAAFWYQKSADLGNANAQFNLADM
                     YFYGDGVGKSLEQSVYWMQKAAEQGYGKAQNQLGIYYRDGIGVAADPVKAYAWFTAAK
                     SNGFEKAASNASDLEKSMNPEDLSKARTLGQQYTDNYKAKK"
     gene            35020..35739
                     /locus_tag="AC06IO_000372"
     CDS             35020..35739
                     /locus_tag="AC06IO_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873589.1"
                     /translation="MTERKKTTVKNKKAPVKKSQLTVSLLIFIDLISILLSLMPGVAA
                     FSLTGLFLYYGFSKTWLVLICSPFVLLFSFILIIALIRISLPKLKPGRYKRELNKTMI
                     AWFCHFALSRAAKIVGLIHLLQSFNVIKYLYWRALGIKVSFRVMNSFDIDFVDCPLIS
                     IGKDVTLASQVSISCHSDVGNYLFLSPVVIEDNVFVGMTTKIGPGTTIKKGAWIGYGN
                     AFVNQVIEENAKIGNVRPVPE"
     gene            35877..37376
                     /locus_tag="AC06IO_000373"
     CDS             35877..37376
                     /locus_tag="AC06IO_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873590.1"
                     /translation="MKKIVYVLLFISSLTHANCVIYFNEQPLNKRLSDPLYSLLSNTE
                     ICPQSIQELRNSMRLRGLKEVIGMVANRGRNNPAEGSFSFFESVYGRLATDQLIAKGE
                     LFLGYFTRLKDGLIILDQKPRKGKLLIEAISWDAKKALYNFYELRGIEHGGVRWFYRG
                     NSRDAYKDNTWLYRISPEGENHFGSRMRCSACHNSGGPILKEQSLPHNDWWTNQRPLI
                     LLPNKPDGDVSQLLEQISDVSVFANDVITGMRHLSDSKKMNAFQGKLSLQEQLRPLFC
                     TTEINLQSSTDSSETVVSIPSAFWLNPLLGDIHLTISMEDYNQLLKEFGTRFPETSLQ
                     DADHAWLTPVKGEADLNAIKQLIQHNIISKKFAQSVLMIDFTNPLFSSKRCELLKLIP
                     ESYQTNSKEWVEQFLINLRNSEEQFKGARQLADYMSKEQLDSKELMKKIKIYEYFLNQ
                     SISTRSGLSKHFQHLIELRKAVFRSELSQNPLGQILEPGFRVIFPESQQ"
     gene            complement(37442..38563)
                     /locus_tag="AC06IO_000374"
     CDS             complement(37442..38563)
                     /locus_tag="AC06IO_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873591.1"
                     /translation="MAAQRHLYIFTLIGLLLGVVVDILIRYNNTTAFIYSVVTIFGVL
                     FALTYNNVNFSRLIGTSFLLAFFLSIPLFPLKMDYSMKDYFHFFTFFVGFPFFIYVAH
                     CFHYAYHHDNTWRVSYSSLFAGVWNTIPLLFIALVFSSLANLLIVLGSFVFKTVGNNY
                     LWDLYFYNRDFKLISNTTLFFMGLGVGQQNLNIIHNMRFLLLRIMYYLFPLLAAISIL
                     YFILYTFHSFSSGQEHINPLIVLIPLTTAGIIFFNAYFQDGTIKSDYPSWLKLSLRVY
                     RVILFLLALMMTYKILSDFSLDTNAFIYLLVAVLFSFTYAITAFLNENQEKQWIYMGN
                     IGTAIFFIVTLFLCNLPYIPVEFTIGGGNAINFITSNLS"
     gene            complement(38724..39809)
                     /locus_tag="AC06IO_000375"
     CDS             complement(38724..39809)
                     /locus_tag="AC06IO_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIO9873592.1"
                     /translation="MKNLYIDPDITKASTISSEFYTSTEWFETTKEKIFSKTWQFCIS
                     TEELTTNRQLIPFTLLPGFLEEPLLFTKDESGTIRCLSNVCTHRGKILIEEPCVAHKI
                     KCSYHGRRFNFCGELIHMPEFELTKDFPSSNDNLSQISTGILEPFLFASLSPIVPFHE
                     VFAEIKERLFWLPMEKMRLDKEKCKDYVVKSHWALYCENYLEALHIPFVHPSLRKVID
                     CNTYTTELFRYCNLQLALASPDEVSFNLPKGAQDYGKQVAAYYFWIFPNTMLNFYPWG
                     CSVNVVKPLSPELTQVSFLSFVLDESKLGEGAGGALDQVEQEDERVVESVQRGIKSRY
                     YDMGRFSPTKEQGTHHFQRLLCEFLND"
     gene            40017..40991
                     /locus_tag="AC06IO_000376"
     CDS             40017..40991
                     /locus_tag="AC06IO_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HIO9873593.1"
                     /translation="MRSSRFLCLLNGMLLSSNVAAFSPSAPIFSPYVDLTINTHWDSQ
                     SQDMEPMDLISPAQKLGIKAYHLAFITDSGQCQPAWGAQQNYSVAKGWGKKQFDTLSR
                     EGVKLTVSFGGASGTDISYHCDKNQLINTFNQVANQYHANALDFDIENGTANIPNLLQ
                     ALKLFQKEHPDVLLSFTLPVMPEGLTSVGKEIITSAATLGLHFNVNIMAMDYGPAYSG
                     DMGDYAISAATNLHQFLQEIYPDKKPEALWQMIEVTPMIGVNDVNTEQFTLSNAAQLK
                     QFAQKNLLGGLSMWSFNRDKPCADKWASPICSGNNLQSHDYEFVKYFQ"
     gene            41238..43010
                     /gene="lem20"
                     /locus_tag="AC06IO_000377"
     CDS             41238..43010
                     /gene="lem20"
                     /locus_tag="AC06IO_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem20"
                     /protein_id="HIO9873594.1"
                     /translation="MKSKEERFNEWRQEKAKSEQAYIKTVPLVEEYIKSFDTLAELRN
                     DVANPNSEKIKKMDAHKRRTLGVLLYAKLKLDEVIEKISDSDSQEKDLLIELNNTLSK
                     AYDNPKSDAKNRVKILIETTEHLLSIEHHSYPQLAKELCTDLYALLCIAKRSLKKNEI
                     LAGWFIDVHEINKKLEELLAKTEEKVEILDKILSEKLNESLETSQKDRTIQEHFNERF
                     TEILSGDEKQRTKLDKLKMSLKEVAEGLSGIVVLRKEKVTIGEKIKKIEALLKAVEEN
                     DKKVTGRQYFLDLLASHKDHFNTLMSTEGGELKKQLAGKIEQLKNPDFYQTVSSTLLW
                     GASWATSLATVIYRQITPQAVQDRVVAYAPATLDSEAKALLQELARQQLIELKKQQSL
                     KRDELIQRYSQISSGSPELEKLIINATSDHLEQIAAATTETSKVLDGYDQIEIKLKDN
                     VEKLKGNQLTRKQLSVFIEKNDTWIVKLSNWLAENIHEIFKSDTARMIDKARKVQQKL
                     GEFQRQYQTEIESEKAKIENNDSLSSEMKVFLKNQFEETKNAGGMEQKPQKNLSKETL
                     QKLFVSLLPENEEISSGVIFSPFN"
     gene            43031..44626
                     /gene="legA2"
                     /locus_tag="AC06IO_000378"
     CDS             43031..44626
                     /gene="legA2"
                     /locus_tag="AC06IO_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegA2"
                     /protein_id="HIO9873595.1"
                     /translation="MSQSVDQSQVIDRLNRYLKWHNMPVSMNEEGICNGLVTVYAKYL
                     LEGKEKRFFELLDQIVRMEEDSAIESEVNQFVYDVVLTLFPHQFNKKLSQANSMCALT
                     INNKPLVSSFDFAIATQDKNWEEIFKNLALQEDEIARVSGTAHAVAVRKKNNKYIVYD
                     PNYSSGTKEFSNEHELISELHYNVFGYHNGFYIKGGLLGMMVSVIRHPDNKKPRAFPQ
                     VTQLYDRYLTENNINDVAVSFHGGTFNTLEHAAMVSHAEVIKNLLEKGAKDNDHQAAG
                     NAVMYNNVEALKALLGKNRETELFQRLFHAALESGREEVYDALFSMKGALPFNSSVQA
                     IRAAAKGGNSVLLAKVMNYFKRNHTGFSDLEDAIPSAIASGSVQCVKLLVNQLNESKK
                     PLDDKKRMEYLLESIKQNQSYMVAYFLKDIPQEHLKTIYMSVSAVERTNLHILRQLQN
                     YGVPFSDMSKAIFEQKEHHFVGLRRILGVFLCKFTDFLKELLFNDCGIHCHRADESGV
                     KKSFFAFPKKLVGVEQLQIPKLY"
     gene            44705..45631
                     /locus_tag="AC06IO_000379"
     CDS             44705..45631
                     /locus_tag="AC06IO_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIO9873596.1"
                     /translation="MNILLAGGSGLIGQELVHGMGEEHHFTVLGRDKDAIQKIFTTKA
                     SSLSWDELNTQDASQFDAVINLCGVNIAISRWTPKIKEEIISSRVQTTENLLHWILQQ
                     NAKPRFYCANAVGIYGLQENGDATDLTEDTAINWEHPKDYLSEVGIRWQQALQKAEEA
                     GLPVTSTRFGVVLKKGKGFLGKLYPSFICGLGSTIGDGRQYLSWIDSEDLVNAYRFLL
                     NHPEITGPVNVTSPNPCMQREFAKAFAKSLHRPLLLWTPAFIIHLLFGEMGDCLINHG
                     QKVKPKRLLEAGFEFIWPDIEKALKHQYSAIQ"
     gene            complement(45725..46141)
                     /locus_tag="AC06IO_000380"
     CDS             complement(45725..46141)
                     /locus_tag="AC06IO_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HIO9873597.1"
                     /translation="MMFQSKLATFFGALILAGSAFASQQGACPDINVIKAEGLPMAEE
                     IGMNYYLAYNISDYSTSSVWGFFIAPIEADSEEGALEASNEVLSMMNAPGVPHQQDED
                     IICSYDTGTPNLIAAAIKDGSQISPMKLKQYFKSAR"
     gene            complement(46418..47692)
                     /locus_tag="AC06IO_000381"
     CDS             complement(46418..47692)
                     /locus_tag="AC06IO_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylpolyamine aminohydrolase"
                     /protein_id="HIO9873598.1"
                     /translation="MAKDKGFFKKAKTGKSKSSATPYAEECAIQIPSEIDNEQMHRMP
                     AGGEEDQYLRIKHMSALIKKYGNLPIITTRETRLPDYWLDLFTAIDEGDTPKAHALFH
                     LLPQEDIILRALRAVHSEDYLYQLIKYCIQAKHFGFKQLNADLVVTPKTFEILIKDCA
                     TTLFNPAKTHFSFGLPSHHAYTQMGSGFCLINKTAMLIKQAELSSVQPPRFVIIGTDV
                     NRDNGLCDILRHSFSHLSICHIDVFDSRVYPQQDFAYINNEFNSEGVDVGKNIHVWRH
                     NNLNYYAVDLSLTSRKSVGVHPALIFALEQLKESIREAKAKGQKIALYLPTGWDSHED
                     ETAYCGKFVKGRMLGKTAAQQFRFNDGDLSYFYESIFTLYNENKDCIDTIYWGLEGGY
                     DRTMYERELTLLLQVIEKQLLPQDSNSHNMSC"
     gene            47892..48296
                     /locus_tag="AC06IO_000382"
     CDS             47892..48296
                     /locus_tag="AC06IO_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIO9873599.1"
                     /translation="MVQHDIIQLSPKPYGAHLITNEVMQKITLRGTGLLHLFIQHTSA
                     ALALNENASPDVLIDLNHFFTQLVPEHQHYRHDIEGPDDMPAHIKSILCGSSLTIPFQ
                     ENKLLLGVWQGIYLMEFRKQAGARTIVASQLI"
     gene            48435..49481
                     /locus_tag="AC06IO_000383"
     CDS             48435..49481
                     /locus_tag="AC06IO_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HIO9873600.1"
                     /translation="MNDHYVSKKKMFAVVFCVFAPNEVLLANDTAVGGEGSLPIPVSQ
                     PHIKMVSELIRISGKNLNESQMNGSWHYNCNFTFKNTLNKEVTISMAFPFPINDGNSE
                     IALPAGQQTNVGQALVYDFLVTVNDRQVPAQRGNIAPDQNKGLYYVDAYFWKTTFPPL
                     ATVNIHHDYSTGATYDVMGYHWVRYVLKTGTLWQDSSIGHTRLEVIPNTPTRLCSEID
                     PKADYLNPTPSGMSISGSRADRKYIWDLRHFQPQADLSLCLFTGISYVRYKVIYPWLN
                     SDDALSKLARLSNKELRFLRNTIYAQYGRQFQSPDLQEYFSKKWWYVPNPDYSDRMLN
                     EEDKKLLSMINQAK"
     gene            complement(49643..50170)
                     /locus_tag="AC06IO_000384"
     CDS             complement(49643..50170)
                     /locus_tag="AC06IO_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIO9873601.1"
                     /translation="MAIFKNLVLTLVSSALLVTPCFSAGLTLESPAFASNAAIPQKFT
                     CSNLNYSPPLIWHDPNLGTQSYVLIVHDPDAPTGNWIHWVLFNIPAQVKQLSEGTTTP
                     AGATSGLNSWNTTGYSGPCPPAGTHRYYFTLYALDTYLNLGPEATAQDVVNAMKGDII
                     DTAELVGHYSKNASQ"
     gene            50487..51500
                     /locus_tag="AC06IO_000385"
     CDS             50487..51500
                     /locus_tag="AC06IO_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIO9873602.1"
                     /translation="MKAIGYYHSLPITDNKALMDLDVSVPKATGHDILVEIKAVAVNP
                     VDTKVRRNVQPEPHQPKILGWDASGVVTEIGSEVTMFKPGDEVWYAGDLTRPGSNCEF
                     HLVDERIAAKKPKSLSFQEAAALPLTSLTAWELLFDRLQLSAEDKGILLITGAAGGVG
                     SILVQLARQLTQLTIIGTGARPESIQWILDNGAHHVIDHTKDMKAQLQNLHYSEVDYV
                     ISLTHTDQHAKSLVEVLKPQGKFALIDDPQDLDIKLFKLKSISIHWEMMYTRSMFKTA
                     DMIKQHDILTEVARLVDAGKIKTTIAECLGLINADNLRRAHALLESGTSRGKIVLNGF
                     KSI"
     gene            51843..>52090
                     /locus_tag="AC06IO_000386"
     CDS             51843..>52090
                     /locus_tag="AC06IO_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HIO9873603.1"
                     /translation="MIIYLLWLLAVTSFNFSTSTIPVSKAEPQGNLLYSEIPSIKMPL
                     NEIKTLLQKEGNSLQPAVIDKVITTIQCANAYQVDRNN"
BASE COUNT        15725 a         9548 c        10501 g        16316 t
ORIGIN      
        1 atatgtacaa gttcttctgg gtgttttcat tggtattttg cttggatatt ttttgcctga
       61 tgtaggggtt caattaaaag ctatagccga tgtttttatc aaaatcatta agatgctaat
      121 ttctcctatt atatttctca ccctggtctc cggaatcgca gcaatgaatg atatgaggca
      181 agttgggcgc ttggcaggtg gggcattgct tttttttacg ttcatcacaa ctctggcatt
      241 gctgataggt cttctggcga cagattattt taagcctggt gttggtttaa atgtgaatcc
      301 caattctctg gatgccaagg aagcgagttc ttactttgag ggggcacata aggtaaccaa
      361 tgtgactgat ttgctaatta atattatccc gagaactttt attagcgctt ttgtagatgg
      421 tgaactgctg caagttattt ttatttcaat attgtttgcg atcggcctga ttcttacagg
      481 caatgcaggt aaatcattgg ttactggaat gcagaccctg tctaatgtct tttttaaaat
      541 cattcatttt gtaatgtata tggcgcctgt agctgccttt gccgccatgg cattcagtgt
      601 cgggaaattt ggagttggtc ctttgtttaa tttaattgga ttattggctt gttattatct
      661 aacttccatt ttctttatct tgattatctt gggaagtctt ttgcaatggt attgcaaaat
      721 taatatttgg catttgttac ggtatttaaa ggatgagctt ttaatcgtgt ggagcaccgc
      781 ttcctccgaa accgtactgc caagtttgat gcagaagttg gaacgattgg gatgtgagca
      841 atctgtagtc ggtttgatcc tgccattagg atattctttt aatttggcag gaactgcaat
      901 ctatttaaca atggctgcca tgtttattgc acaggctacc aatactgatc taacagtttg
      961 gcaggagtta tcgcttctgg gagttatgat cattagttcc aagggtgccg ctggggtgtc
     1021 cggaagcgga tttattgtgt tggcgagctc attagcaacg ataggacaca ttcctgctgc
     1081 aggcgtcgtt cttgttttgg gtatcgataa attcatgaat gaagccaggt caattattaa
     1141 tatgatggct aatgcaatcg ccacgattat tatttccagg tggcaacaat ctttggatta
     1201 ccagaaaact cgatttgaat tgcaagaaaa aggacaagca gaaaccaaaa gtgtttatgc
     1261 cagtgagatg taagatttct acaggactca cgaaaaaccc caatctcatc gttaaaaaat
     1321 gtttttaatt cggtcattta gtttatctaa actccctcat taaaaacatt ttttgcctcg
     1381 actttggagt ttttggacag tcccgttcta ttgcgaaagg ggtcatgtaa ccccacaaaa
     1441 aaatcaagct tatagaaaaa cctgcatgtc cttgtgttgg gcatctagac cccaatcaat
     1501 tgtgtttata tttaccaata atccctttaa attaatttag gctggcttca ttataggttc
     1561 ttctgagttt tccatttttt ctcttttagc ttgttttgcc gaactaaaaa ataaactaga
     1621 agcgttgtaa tctgcagtaa gttttaattg attaggcaga tcatccatca tgtaaatgag
     1681 cgcttctttt gcatcgagct gtcttatcca atctctggat aaagtagatt gcattgcttt
     1741 gttttcaatc agtttaaata atcgataaca agaagagtca gtctttaaca agaaacaatg
     1801 gatttttgcg aactctgcaa gttgttgaaa ggatttgagt aaaagggtta tttgttcagt
     1861 agtctcattt gtcattttta atttattgat ttcctttatg ctacagataa tccgaatttg
     1921 cgttctacaa acttgcaaac tgatgttgta tcgatagcaa tcaagaagat ttgtggccgt
     1981 taatgttgta tttgtttgag cgctttttaa aaattcggtc gtattgttac agtattgagc
     2041 aagaattttg agatagtatt ttgtgccttg agggttgatt tcctgttctg cttttccata
     2101 agcaaatact gttgcggact gggagtcgtg ttctttaata aatctattaa tgaagtagtc
     2161 aagaatatca acgaatctga aattattgga gttataagcc atatcaagag tgatggcttg
     2221 aaaaatgtta tccaattctg tatcaattac ctcaaaatga tcgctgtttt tatagatacg
     2281 agagttgggg gcttccttgt ttagcaaaaa catttggttt tcaagatcat caaaaatgac
     2341 tttggtatcc tgattaaagt atttttgaat aaaaagtcta atcttaaatt tgatttcatt
     2401 gcttatttct ttgctatcag attcggaaat tgctgccagc tgctttaaca acagcattct
     2461 gtagagaacg aattcatcag tgacgcgcgc tgattgttgt aagggatcca agcctaatcc
     2521 aggttttatt ttatggcaaa gcgataataa aaaggcatag gagttatcaa gataacgatt
     2581 gatgttttcc gctgttagag gctccctggc atctcttctg gcgataccta acatttgtcg
     2641 atattccggg tctcctaaaa aaatacaagt cgaagtatat aaaggctcat attttttaaa
     2701 cagattcaga gctgtatcgc agagataatt ataggggtta tgcctggatt tatctggcgc
     2761 atcaatggga gtcaaacgtt ttatatgaga aaaatcggat tcactacctc ccaggccacc
     2821 tttctctctg gagttagaat agaatttcaa taacataggg gggatgtaat tgagttgaca
     2881 gacaatctca aggacttgct ctttcatttt ggggattaat tcaaattctt caatcacaga
     2941 ttgatttttt tcatcctgca aataagtcat ggtatcgtga atccacttaa tcaataactt
     3001 ggagtttttc ttgttaatcg aatgaatgag ttcttgagtt tgtatcctgc tttgagtaat
     3061 taattctttg tattcatccc ccaaatcaat ttcgatggct ttactgtgcc tataccgaga
     3121 agcgtattct cttagccatc cagatactga gcctctggct acctttttac caatcgtgac
     3181 taaatgaaaa ttctggttga ttttttgaaa atctcttgta ttttcaataa cctcactggt
     3241 tgtttccttt tttaataaac tttctttttt caaacgtaaa aactctccat ttatcatgtc
     3301 gatagattgc tgaatatgag ctctattatc cgaagaacgt tggtaagtta atacgctgag
     3361 tttattgatc aggaggtgcg ttgctatgca agagagagca aaaaccatga cagccagagc
     3421 agctatcgcc catagaaaag taccgccagt cataataatc atagtcacac cgactattgg
     3481 agcaataatg actcctgcaa agagtgaaaa aatgataggg tatcttgcga gtatcccttt
     3541 ctgattcatt ttttccagta aaaaatctct tgccagaagc atgaatttca actggatttc
     3601 ctgagtaaag gccaatcgaa cctgttcaga ctctgacatt tgattgatcc acttgaccag
     3661 aggcgattga tgttcacttt gaatttccac atcgtggact tgataaagtg atttaaaacc
     3721 tgagttaaaa atacgactta attcacgtgc aaaatagatt tcgatagcca aatgaacaga
     3781 ttcgccagat tggttggcaa tctttgcttt ggcaatgaat tcctctctgg aattgggttc
     3841 gattggtgat ttgcttattt tcccaagcag caacatttct ctgaaatgaa ataatttgat
     3901 aatttcaatg gagatgcgct cacattcttt ttttatcaaa ttggataatt cagtttgttt
     3961 ttccagggtt tcattaaatt ctttttgtac attctctggc gcccctaaaa attcattatt
     4021 gaagtaatcg aataacaggt cggtaattga tttgcctaaa gcgagagaaa agacctcggc
     4081 ggctacaaaa ttgaataagg tattgtcatt ggaagaattg atatcagaaa gaaaatttct
     4141 tatagattcc ggaataagca gttttgcttt ttcaatgtag taggaagaac ctgctgtttc
     4201 cttttccaat gaagatattg tgcttggagc tcggcttaga gcgatagtgt ccagatagtg
     4261 attagctaac aggcgcgtgt cttcaatttt ttctatccag gaatataatt tttcaggatg
     4321 ttttgattgc tcaaaatata ccccaaaacg ggttatcatt tggctttcaa gtcgtttctt
     4381 atggttatga tgaatagcct taatgactgc agtttctttg tcgttcagtt ctaacattct
     4441 tcgctccctc gtagagtgat tagatatgac tttatgcaat aagggctatg ccaagagact
     4501 atatttgctg ttgcttgttt cctggcattt tcctgccaaa attcttcaga atcttgatac
     4561 atcacatctg ttgttcccgc ataaacactc agagggagtc cacagaaaaa ttggtggtaa
     4621 tttttcagca tgcccttgtc aaaaagtgaa agcagtcatt actgaaaggg caagacaatc
     4681 ttaattatta tttaaacgta ctattggctg attgggtatc cttgaaataa ggataattca
     4741 gcattaatga gccagttttg tgccatatag cctgtctccc gcgtctccta aaccaggaac
     4801 aatgtatcca tgatcattca gctgctcatc tatggctgcg gtgaatattg ggacatcagg
     4861 atgttctcca tgaaaattgg aaattccttc aggcgcagcc agtagacaaa gaaatttaat
     4921 tgacttggga tgtaatgcct tgacctcttt aaccgcggca atggctgaat ttcctgtcgc
     4981 cagcatgggg tcaacaacaa taacgtctct gtcttgtgtg tgttcaggta gcttgaagta
     5041 gtattcgact gcttccagtg ttttgggatc ccgatagaga cctatatgac caattctggc
     5101 agtaggaact aattgtaaca tgccgtccag tagaccatta cccgcgcgta atatcgagac
     5161 aaaaacaagt ttttttcctt tcaaaacggg tgattgcatg gtggccaaag gagtttctat
     5221 ttcttcgtat tctatttcca aatcccgagt gacttcatag gctaatagca tactgacttc
     5281 gtgcatgagt gtccgaaatt ttactgtgct ggtttctttt ttcctcatta tggtgagttt
     5341 gtgctgaatg agaggatgat ttataacctt aacttgattg aagtccatta tgattcctta
     5401 ttaaaatatc gtgccatcac tgataatttc tattgggggt gttccattaa ttcgtttctt
     5461 ttgtgcaatc tgtcgtcccg cctcccaagt gcctccttgc agtattttgg ctaagggaag
     5521 cgattctgca ctcatgttga tctgttgcct gattaatagc gctaactcat ccagcaaggc
     5581 tacggttaaa gcacgccatt ctactatggg ctctgagcca gggtcttgag gttgctccag
     5641 aattttatta tttcttactt ttaatagttc ggtgtcgatt agtaaaccac catttcgata
     5701 ctcaggcaat ccggtcaata catccaaatc agtaacatca atgccggttt gttccagtgg
     5761 ctctactaga gaataagtga gccattgcga taatttgtga aagggtatgt attcagatcc
     5821 ggcttcattg gttttcaatg cgctgtggat ccagacatcg cctaaagata ctccatgata
     5881 gatcaatcga acaggccata tgtcattgaa tgcttctaac acggcctcga atagtttggt
     5941 agtaggcagc attttattgt cttgcaagga ttccacataa gtataaaact caccaagacg
     6001 ttttcggcga ttaaaacagt attcattgcg atgaatgatt tctccaaggc gattaagcaa
     6061 ggatactcga ccgggtattc cttcaagcag attagtagag ctaacctgaa aaccttttat
     6121 gagctctgtt tctttaaagt caagcagacg ctcggcatcc acgcgaaacg ggttagttgg
     6181 gtcagcgcta aatgccccac tttcatagag gtataaactg gccagggcta atccttcaga
     6241 gcgactgtac tccttttcag tgagagcttc tttatagcgc cataggggac ccgcccctgc
     6301 atcgaggaaa acactgatga tgaccagttc gtagagtatt tttccccatt ctgaggggga
     6361 aattggctcc agttgtttgc gtaaattatg aattcgagga aggccatcca tttcaaaatg
     6421 acgccagcgg ctgtgatatg gaatatcaag gtcaggataa cggctagtga taatgtctac
     6481 gatatacgat gctgttgttg tcattcgttc agggttcagg gaaaagtgtt ccagccgatt
     6541 ctgttttgcc aagtttaaaa tacgatgcga ccgggttctt atggtgcgag gatcacgaag
     6601 cgtattcaaa acattttgta tttgctgctc ttgattattc atgaagggca cgtcctttcg
     6661 gtttagccaa ttcgttgata tcaataattc cttcgggtga gtaatatcct gctgcacgct
     6721 tggcatctat ttctacccgg gcatctggag gaatcaactc atccggtatt tttacccgtt
     6781 caataacatt gattccagat ttgagcaagg catcgtattt catattggac attgaaacga
     6841 agcgatgaat tttagtgatc cccagccaat gaagaatatc ggacattaat tcctggaaac
     6901 gcatatcctg aacaccggca acgcattctg ttctttcaaa gtacttggct gcagtgtcac
     6961 cacctttctg tctttttctg gcgttataaa ccagaaattt agtgacctcc ccaagagctc
     7021 ttccttcttt gcgattataa acgataaggc ctgcgcctcc tttttgtgcg gattcgatgg
     7081 ataattcgat tccatgaacc aaatatggcc tgcaagtgca tatgtcagaa ccaaagacat
     7141 cggagccatt acattcatca tgaatacggc aagttaattc gatttccgga ttatggatgg
     7201 tagtcacatc accaaagaaa taagcagtta atcccccaat gggtggcaaa aagacatcca
     7261 agtcgctacg ggtcaccagt tcagggaaca ttccagctgt ttgttcaaaa agagtgcgtc
     7321 tgagcaatgc ttctgaaacc tggaagcgct ccgcaattcc aggcaagtac catactggtt
     7381 caatcgcggc cttagtcacc acgacatccc ctgtttcact taaaattttt ccatcaggtt
     7441 ttaatctgcc tttttgcatg gcaatatgca attccgggat attaatatgt gcccttgtga
     7501 cggcaatggt tggtctgatg tcatagccgg cttttatttc ctctgagaaa acagaagcca
     7561 ctagatgccc ccagggatcg agggagacaa tttttcctgc ttctccccat tgcttatgag
     7621 gccctatatc aacaggaggc agggtatcgg tcagatcagg aacgtgttcg ggatccagta
     7681 gaccagctgc tactgccaag gccctgtaaa cagagtaaga ccctgagtga gttccaacgg
     7741 catttcgatt tttgatattg gtgagagaag caataacagg ccccctctct ttagggtttt
     7801 tttcacccca tttgattttt aatggagctt gtgtatggct tgccgggtgt gaagacaaga
     7861 caatgtgtcc ttttgatttt ttctttgaat catctggtga catgatactt aattcctttt
     7921 gtttcaacgg tgctcaaata aatcggttgt tttacctttt atccctttat aagcaggtgc
     7981 acactattgt tataaactgt ctaaccaatc ataaaccaca gcattggaat aagaaacatt
     8041 gcctacctga caatgacttc cagccccttc aaaatcacta aattcatatt gtgcagaatt
     8101 cgttattttg caaaactccg cgaattgttt tccaggctct cctccttcgc cacttcctaa
     8161 taatgcaagg caagggattt taatatgatt taatgaatca cctacggtga actctcttaa
     8221 ataattaaat gttgccttaa aagtaggctg cccaaatctt ctgattaaat gttctgtttg
     8281 ggctttgagt tgagggggca tcacttcatt gggtatagag ggtaagtcat tgatgctaaa
     8341 atcttcagaa tcaggcattt cacaagggtc catttttaca aaggaacaca tataagcatg
     8401 taaatccaga ataggggagt tggcaattaa aaccttgatt cttggttcat ggctcgcggc
     8461 acgagttgcg aaatagccgc caaaactaat gcccatcaat gtcaatttgg ccatattgat
     8521 ctcttgcctg aactcaaaat aattaatgat tcttttaacc acttgctcaa aatcaggctc
     8581 aaaataagtg tccggatatt ttcgaaagac atccatttgg ccggggcctg cgaaatgaat
     8641 cacattatac ccacgttcta ccgcagctaa tccgcgcatg aggaactctt cttccaaagt
     8701 tccatcaaag ccactgacta tcatgacggt atctcgtttt tctccatcat tggcagggga
     8761 gataaaatag catgggagat tgatgttttt ataaggaatg gaatggtttt caaaatggat
     8821 atccatgctt gagatggctg tgcaaaagca atctgctgaa ttcaacccta attttctgtg
     8881 atgttctgag gaaggagggg aataatattc agcggcacga aatgaattgg aggctttgaa
     8941 caattgctct cgaccgctta tgacatggtt ttttaccatg cgttccaaac cgtccttttt
     9001 ttgccactcg gctaaccgtt caaactgtct gacccattct tcagggtctt catttttcat
     9061 ggtgtttgca atatttaaac attcgccgac tgatgctgct ttatagctgc tggatcccag
     9121 ttgtcgtatt aattgaaaat ccatttctga atcagtaaac cccttcaccc gaatatcgcc
     9181 acgactgata tcactcattt tacttctccg ttatttccgt ttttacagag tgttatgagc
     9241 ttaatagtaa cggtacatgc caagaacatc aatcttaaat acaaaatcct taaatcatga
     9301 taatgcataa aatatggtat aaattttata aaccaaaaga tgttaacatc gcactatgaa
     9361 ttgatttgtt tttttaaatg cgcaaagggt acaaatcaga attgacacgg tctgatatta
     9421 tcaggctaga gaccagctgc tgaaatgttt tgattttgat ctttttcaac ttgttttttt
     9481 gtatccatag attcagtgag cttttccatc gcatgctctc ttttttccgg atttggcaat
     9541 ttattatgaa gttgttttaa aagatgaaac tttgattcat ccactttagt ttggcttaag
     9601 tccatttcac taaaggcaat cagagtcaga ttgaattccg taatcagcga gtcagggact
     9661 ggtaaacaaa aatcagaggc caaaatggtg ttaatttctt cagcaaacga tgttaattga
     9721 cggtaacatt tttgttcttg tgagtagtag gaagcatgtt catactctga ttttgtagtc
     9781 ttgataagct tttcaatgac tctatcaaat tggtttttta agatttccag ttgggattct
     9841 gaataatggc tttcagaagc taaggactgc aggttattca tcaccctaag ccaatatgat
     9901 aagcgaacgt agttgtaggc cacatcattg taatgaattt tttccaataa tgatttgaat
     9961 acatccaaat tttcatcatc aaaaacaatc ccggcattct taaatgtctc aagcatcgaa
    10021 ttaatctcgg aatagaatgg cttgtcgtaa tcattcaacc agctgtaaat ataaggtttc
    10081 gcttcatctc ctaccaactt ggcaatggag caataatgac cccaatcatg gggttttctc
    10141 tcatggaggg tctcattcgc tttgtttaat acaggcctaa agtagtgttt taatttgaca
    10201 tctggaatgc ctgcattttg gagtgtctta agtaacatgt agctgtcgta taaattcagc
    10261 ctttcatttg tgtaggtgtc tatcagttta gaaactattt ccaataagat tgattcaatg
    10321 ttatccctta ggtgattgtc cttatctatt aaagtcatta tattgtttag gttaatcatc
    10381 tgatgtaagc ctgattgcaa ttcatatttg ctccctgtga tgaaagcatt ttgatcatta
    10441 ataacttgta ttgccaattc attcactttg ttgcgcaaga aatgtatttc tccctctaaa
    10501 cctaattgct gacataaaat aaaggcttgt aaaaattgat ttttacacaa aaaattcctt
    10561 tcatcagtgc tgcaatgttg gatatcaagc gtcgttgtta tttcttttaa tgaggttgag
    10621 gcaatatctg ataatttgta agaaatgact ccatctgagg agtatataaa gcgtaataaa
    10681 tcatccttta ttttatcaga gcacaaatct ctcaaacgag ggctatattc tagtgctaaa
    10741 aatgcttctt ttgaaaatgg agataattga ttgtcttgaa gccttttgaa aacagcctct
    10801 tcatattctg ctattatttt ttctttagct ttggcaatgg catcagtaat tttctccctt
    10861 aattcaacat tgtagcttaa atcagctggt tgattcactt ttgccaatat atttcctttt
    10921 gttttgtaca tgcttaaaac ttttatgtct tcaatactca tgttataaag aagttcatcg
    10981 taaaaatcat agctattatc tctacccata tgggacagag catttctcat gaaatcagaa
    11041 gacatctcca cccacatatt aacaatattc tcatctgtta atttttctgt atcataaggc
    11101 gttaacttat tccttaagcc ggaatattcg taaattggta aaaattggtt agggccaaag
    11161 cgttcgagga atagtgtttc acccctctct tgtccatagc gttcataata agctttacga
    11221 gtttcttcat attgtctcgc atactgcttc tgtaaataaa tcgcttgcaa gagaggggag
    11281 aggggatggg ctggattgaa atcattatag gcaatttcat ttcctatggt tgggtcatta
    11341 gtaaagaaaa tggcatcgta tttgttaatg tcatataaaa gttcaacgta atttgagcca
    11401 agttgcgttt tcttaccaaa taagcccaat ttcattttat gatgaagatc atcaagttcc
    11461 ctttccagaa tttcacgaga ctctttgtat tctttttctt gtaaatgggc cagatggttt
    11521 ttttgacctc ggccactatc aaaatcctca gttgaaattc tcttaatttg gcctatatcc
    11581 ggattgaaga tcaacatgcc tgcagctgga tagactccac ttccataacc gagtatggag
    11641 gttgagcgca gagggttttt gtatttgaga ttaggtgctt cttttctgct tttgatgtta
    11701 atattgtcag agcgcatgga ttttcgcatt tctctttcgg ccaatgtttc atctgttaga
    11761 gtcggtttat tgggtctggg tgtaggcatg gcacttggtg tagtaatgcc acgggccaga
    11821 actctccctt ctctcaatgc tgcagcaaga ttttctccag ccattcctgc tgtttcatcg
    11881 aagggagtaa gattaaccaa ggtcatagcc tgtaattctt ctatagataa cagttttcca
    11941 tacaatgtgc tgagtattgg ataagcttct gactgcatat ccctgattat gcgggcatga
    12001 gcttcttcat tttcataacc tgctttgatt tctgcttttt tagtaatgta cgaatcaccc
    12061 tggctataga taaggcctct ggcttctacg acaagttcgg ccatttcctc taaagcgaga
    12121 ttgtctttgc tggctatatc cttataaaaa tcaagatagg tggcatcgta ttgatttctt
    12181 gcccttatga tatcaagaca atcgcaatca tgaattattt tttgatagat attttttgca
    12241 ggaaaatctc cttcttcata cttcgcaaac tgccagaata attgaccata ttcgtcctca
    12301 atgagttgaa aatagccatt ttccggactc ggatccttgt tcgcagttgc ttcagctaca
    12361 aatcttgctg tttctgcatc aatatgtaaa acgcgtgtca gataatagta taaaaagata
    12421 gcactttcat gatcccataa atcttcatca tctccttctc gggccgaatc atggaataac
    12481 aaagcgattt gtatgagttt gatgtctgtt tctgttaaat tttgggcttc ctcattacca
    12541 tgtctcctgt ataaattggc aaaggcacat gcatagtttg cagctcgggt aacatgttgt
    12601 ataccatgat gaaatctagc tatagttgat tcctgatgcg agtccggata ggaataacga
    12661 taaatattgt cataagccca cctgacatga tcgcccaaat cattttcaag atttaaatca
    12721 ttcgtttcta aagagtcaaa aattgtatat ggtattcctt ttgaatcgag tagttgatga
    12781 atatagggag gttcccacgt ttgttcatct tcgtaaggct cctcatttcc agtaagaatt
    12841 tctaaaggtg atggttctac aagagtgctc tctctcattg agaaatcaat tgaaccctca
    12901 ttaaacatga cgtaattaaa taattcacga gttttaccat tcttggataa ttcgttaaat
    12961 cgctctccta atatccctaa aatatccagg cgagttgttg atgtctctcc ttcttgctgg
    13021 taatctggac tctcttttaa agcatttaat ttttcgataa cagtttctat tacatgtaaa
    13081 tttttttgcg tcactttggg ttgaaaaaaa gggtgtttag cattacgcaa tccaaagcct
    13141 aaatacttgg caagcttgtt atcctgtccc cagctttccc tgctctgttt taaactattc
    13201 aaagtcagaa tcagagcatc tattgtatta tttccctcat atctgtggcg ttcataaagc
    13261 tcaatatatt cgttcatttt catcgtacca aaaagatacc ttagaataaa attatagcaa
    13321 tttaattaat cattaaccaa aaactggcca tgaagtaatt taaaagctat ttaaagagta
    13381 ttccggctaa ctaaagtgga ctacaattaa atttgcccga ttgagttgag agaacatctt
    13441 ggttaatatg tcatttagtt atttttaata tatgcctgag atactctata accatgtata
    13501 ctataattgt ttgcttgttt atcgctatat tgctacctta tctggcaaaa gttcctgtag
    13561 cttatgccat gcataaggcg ggtggctatg ataatcgtta tcccagagag caacaagctc
    13621 gtttgcaagg gtttggcgca agagcactgg cagcacatca aaatgctttt gaatctttac
    13681 tgatatttgc aacagcctct ttaactgctt tggcgactaa ttcagtcact ttaatgaccc
    13741 aatatttcgc tatcgcctat attatttccc ggctatttta ccatttattt tatttgctga
    13801 attgctcttc cctgcgctcc actgtatggt ttttgggtgt aatatgctgt ctttctattt
    13861 tatggcttag tattccttaa attttagaaa ggcatatctt tattcaaaaa attcaccaag
    13921 tgatgattca aagcacaaaa actcatcaat ttattatcta attggttata gacacttact
    13981 ctttatcttt gactttgata tcttaaaaaa gaagttccag attttttaca aggatgtgaa
    14041 tatgtctgac ttacaacaat tgcctaagac tatcctgaga ttttcctggc attttattaa
    14101 aaaaagtccc tggctttatg ccttgttttt catagcccca gtggtgatgg tgcttgaaac
    14161 gaatgtgatt ccctatgctt taaaaatgat tattgatggg ataacaaacc ataaaggggg
    14221 aaaagaaagc atatttcaag acatcgcacc ggctttgtat ttgggcggag ttgcctggtt
    14281 tggcgtgatt ctggtgttgc gcttgcataa ttggtggcaa gcctacctgg ttcctcaatt
    14341 tcaagccaat atccgcatga ccctattcgg ttatttaata cagcattccc atcatttttt
    14401 ttctaatcaa ttagcaggca atttagctaa taagataagc gatatacccc gatcactgga
    14461 agccattcgt aaaattgtat cttgtaatgt aaacactact tttgcagcaa ttgtagtttc
    14521 aatcgtttta atgttaacca ttaatccctg gtttgcgttc attttgttaa gctggatcat
    14581 aattcaatta tttatcagct tttattcagc taaagagatc aataagcttg ccagggtgaa
    14641 tgcagaagat aaaagtattt taaaaggaaa gatcgttgat tccttgaata atattaatac
    14701 cgtgaaatta tttgttcaaa atgagtatga aaaaaaatac gtttggaaaa cacaaaataa
    14761 agaggttcaa agtaataagc gcctggttct tttcatcaat ttttttcgcc ttttagtcga
    14821 tgtccctgtc agtattatgt taattgccat gatttattca gtcatcagtt tctggcagag
    14881 aaatttaatc agcacaggtg acgtggtttt tatatttgca acatcatttg ccattatgaa
    14941 tcaaatgtgg agtctaagta attcgctttg tgatttattt gtcgaaatag ggatagtaaa
    15001 gcaggcgctt gccatattat cccaacctat agaagttcaa gatatagcca atgcaaagga
    15061 gttgaaagtc actcatggtg aaatcaggtt tgaaaatgtg acctttcgcc atagtgatgg
    15121 tcaggaatta tttaccaata aatcgttggt tatcccggct aaacaaaaaa ttgggcttgt
    15181 aggctattct gggggaggca aaacaacatt tatcaatcta attctccgtt attttgatgt
    15241 ggatagcgga aggatattga ttgataatca agacattagc gagatcacgc aatattcttt
    15301 aagaaaatca atcagtatga ttcctcagga taccaatcta ttccatcgta ccttattaga
    15361 aaacattcaa tatggtaaag aggatgctga cagggatgaa gtagtaacag cagcttcttt
    15421 agcaaattgc atggacttta ttcagaattt gccccagggt tttaatacta tagtagggga
    15481 aaggggtact aagttgtcag gggggcaaag acaaagaatc gctatagcaa gagcgtttct
    15541 gcatcatact cctatattga ttttagatga agccacttca caactggatt ctttaaccga
    15601 agaatgcatt caagaatcct tgcaaccatt atgcgaggga aaaacaacta tcgttgtggc
    15661 acatcgactt tcaacccttc ttaacatgga tagaatcctg gtttttgata aaggaacaat
    15721 agtagaagat ggcacacatc aagagttgat agaaaacaat ggattatata aatccatgtg
    15781 ggaagcgcaa acttctggct tcttgcctgt ggaacaagaa gatgaggctg agctagctaa
    15841 agaatttgac cggccttatt tggcacaagt gtaatttaac caaaaaaagc aattgtcgtt
    15901 caagatgaac caaatacgcc cgttccagat agaggcttat ctcttacctt gaatttgatt
    15961 gcaagccgac acccatcatc tggatgtcgg cattcacttc taaacacggt ttctcaaaat
    16021 aatttttcaa aaaaatacac tggctttgtc tttgctatca ttggtcataa gcttggatct
    16081 gttgcttttg gagggccagc tataaagcga cgattacggg ctaatttcgg gaaaaaaggt
    16141 ccaatcttga caataaaatc ataagccata agaagagccc ccaaggcaaa tacgacatcc
    16201 cctggaaaac gaagccattg ccataataag gtggtgttat agaattcaag gcttcgggca
    16261 taggatagtc catgttcata tactgccatt aactgcgacc agccaattgg gaaaaaattc
    16321 aggacaatcc ataaaaagag ccctacatta tataaccaaa aggcataaac acccaatcta
    16381 tcactccatg ccttgctttc accagcagca tatctcaggc aaaaatagat taacccgaga
    16441 gcaagaagcc caaatgcgcc aaataaagca gtatgagcat gatttaaagt caagaatgtt
    16501 ccatgctcat agtaattaat cagtggcgca ttcagtgtac ctcctccaaa tacaccggca
    16561 ccaacaaaat tccagaatgc ggctcccaat atgtagagat aagcgagatt gtaggtaaac
    16621 gttccctgct ttttaatcaa ttgtcggtgc tcaatcgcat caataataag aagaaccaga
    16681 ggcaagactt cgataaagga aaacatggag cctaacggaa tccacatttc aggagtccca
    16741 gtccagtacc aatggtggcc agtaccaatt actccaccca ggaaaattaa aatggattca
    16801 aaatataccg ttctctctgc cgcttttctg gaaaccaaac cagttcccat gagcaaataa
    16861 gcagtagcac aggcggcgaa aaactcaaag gattgttcaa cccacagatg cacaacccac
    16921 cagcgccaga aatccgttat ggtgaacgat ttttcaatcc ctgttaaggg gatcatccca
    16981 aagcaatata aaacagcaac attgatagtg ctggcccaga acaggttttc aagcttgatg
    17041 cgacctgtcc agaactcaat ggtggctttt tttaatccat cccacgtagg ccacataccg
    17101 cggaaaacaa taaaactcca aagaaacaat ccaatgcaaa acccgatttg ccacagtctt
    17161 cctaattgta tgtaagacaa gccttgattg cctagccaaa accaggcctt atcaatatac
    17221 cccataatcc ccaggtaatt gccaaggatg gctccaccca ctacgagcaa ggtgacataa
    17281 aacagaaaat caactaaaaa accttgtcct ttggcttcct taccgctaat gatgggtgcc
    17341 ataaatatgc ctgcacttat ccatgaaagc ccgatccaga taatgggagt ttggatatgg
    17401 acgtctcgaa ggaaattaaa aggcagccac gtattgacat taataccata gaagctttcg
    17461 cgctcagcat aataatgagc catgatggct cctaccgcga tttgaagtaa aagaaacaac
    17521 gcggctacaa ggaaatattg acctacttta cgttggcttt gagtcatggg tttgaaacct
    17581 gaaaaaatag gttccatggg tccatgatca acatcactta aatagcgatg ataaatgtag
    17641 ataaccgcac caaatcccat aaacacaaag caaaatgaaa tccaggtcca ataaaacgtc
    17701 cctgatgagg gggtattacc aacacttggt tcgtatggcc aattattggt gtaagatatg
    17761 ttttggtttg ggcggcgagc tattgttgtt aaagaggaat aaataagaaa gtttgcggtt
    17821 tgaagcgcag tatcaggatt taagctatag gcctgggtat aacctgccat aaaattatga
    17881 ttcagtaacg attgactgat ttcagcctgt aattgccgta ttgaggtggc aattgccggt
    17941 gtcaggatgc tcgttttttt attgagatca aggcgttgca gtgagttctg catttcttta
    18001 ttaataacga attgttcact ttctgttaat tcggaatagg ctttattaaa acgaattttt
    18061 gccaattctt cctgaaccaa ttctcccaaa cgcaccagat attttgccgt gtagtcctca
    18121 ccaaaatagg atcccatacc gtataaactg ccatagtcca ttaagtcagc gcgttgaaaa
    18181 cctgcttttc cttccacgat gtcttgtgat gtcatcaccg tttcgcctgt agaggtcaca
    18241 aaacggtctg gtaaaggagg taccaattga taagttttaa cagtgcccca gatgaggatt
    18301 agaaaacaaa gaatggcagt cactagtaaa acccatttta aaacattagt tattgggtca
    18361 aatgagtgat ccttttgaac aggtgtttca tttgcaatag tatgcatcag gctatcctta
    18421 gcggaatggt tcaattcggc attgattata aagaggatct ttacaagaaa caattcatgt
    18481 tagctacaaa actgattctt agaaaaccat acatctccag tacccataag gttgttgaat
    18541 agcatctgct tctcagaaag aagatgcggt tcttttataa ggaaaagtag tgcataaggc
    18601 ataattgatg acataaaaca aaacactgca accgagcgac aataacagag tgtttttaaa
    18661 tagcaataat attaaaagta ccaatgttgc gcgcgatatt tccaggtaga gagtattgct
    18721 atggccatct gcaattcggc caagggtata aagtgtcaat aaaattaatg cgccaatcaa
    18781 ccaggatgtg atcatggcag gatcaaaaac aagagagaga taagtatata ataacactgc
    18841 aaatgcagta ttggttaaca tataaatccc aggtgaggtc gtattttttt taatcgttgt
    18901 tttttttgag acaggggatt tttcttgttg caagcgatga atgatccatt ctggcggcat
    18961 aaagaacgcg cgtaacacat cccatttact ttttagccct ttaccataat acaggatatc
    19021 tttaaccact ttgatgttgg cgtaaaaagg gttccatgaa tccagaggtt cggtgactcc
    19081 ataatcagcc ggtgcttctt ctggttcaaa agtgccgaac agtttatccc agataatcag
    19141 gcttcccgca taatttttat ctatgtattg agggtttttg ccatgatgaa ctctgtgatg
    19201 agacggagta ttaaatattt tttcaaacca acctattttc cgaatggatt cagtatgaat
    19261 ccagaattga taaatggtat tgacagaggc gacaatgaca aacatccagg ttggaaaacc
    19321 aagcagagcc aagggcaaat aaaacaccca ggaagttaat gtctggaaat agccctgtct
    19381 taaggcaaca gataagttaa agtgctcact ttggtgatgg accgaatgac ctatccagaa
    19441 gaaactgttt ctgtggctta ctctgtggta ccagtaatag aaaaaatcaa ccccgatcca
    19501 taaaagtaac caggaaaaaa tcgattgggt attcacatta aaaatagcgt aatgctcata
    19561 taaataatag taactataaa taatcaggcc cttgatgggg agtgcgccaa tttcttcaag
    19621 gataccgctg ctgaaattgt tgactgaatc attcagttga tagacaccgg attttttata
    19681 ataggctacc caagtttcaa ttcctatgag gatcaaaaaa agaggtgagg caaaaaccat
    19741 taaatttata tccataaata tcctttgaac agtgatagac gcttcgcaat aggtattttc
    19801 tattttgcag acgttgtggc ctatgcctta ttttctaata cttttgtttt gcttgtaaat
    19861 aatgatattg ttatcagcct tgttagaaat cattctaaag gatatttgat gcatcaggaa
    19921 catccaggcc aaaccgatac cccaaaaata gatccttggt taatatttgt catattaatg
    19981 ttgatggctt tgatgcaaac tacaaccgat caatatattc cttctcttcc agcgattacc
    20041 acagcattga acagcactga agcatcgatt cagttaacca tttctatgtt tatgttgggt
    20101 ttaagcctat ctcatatctt ttatggtccc ttgtcagaca aaataggacg caaacctccg
    20161 ttaatgttcg gtgttgggtt aagtatatta ggcagtttgg tttgtttttt tgcgcccaac
    20221 gtcagtgtgt taatcttggg tcgatttctt caggggtttg gaattgggtg ttgcaattca
    20281 gtggggcgat ctctggtgag agatttattc accgatcgtt tgctggctaa aattggttct
    20341 tatgtcggtg ttgtcagtat ttttattatg gcagcctcac ccactttagg aggatttatt
    20401 caagagatgg ctgggtggaa agccaatttt atgttcttat ttgtttttgg aatccttgtt
    20461 tggcttttgg cctttagcat tcttcctgaa acgaataaac acttaaatcc tgaagctaca
    20521 aaaatcaaag tgatggcgaa gaattatttt accttattac gtagcaagat ctttgtggga
    20581 tatacactgt gtgcttgctt tgcttttgcg ggaatggttt cttatcttac gattgcgcca
    20641 tttttatttc aaaataccat gggattgagt cccttggaat ttggacgttt aacttttttt
    20701 attgcgggtg ccatctgttt tagcgggatt gtcaacagtc agctggtcat gcgtaaagga
    20761 atatccttta tggtttgtac cggtgttctt tttatgattg ccggaggttt aagcctgtta
    20821 ttaattacga tgctgggttg gtcgcaaatc tattttatta tgatttcaat gacattattt
    20881 agtgctggcg ccggatttac atttattaat gcctttgccg gtgcgtttca tccttttccg
    20941 caaatggctg gaacggttgg tgctttatat gccagcatgc aggatttaag tgcagcattg
    21001 accagtggtt ttattgcggg aattaaaggg tatggtcaat attctttagc tatgatatta
    21061 ctgattttag gtttaagttc tttagtggca tggtattatc ttggattgca agatgacgct
    21121 atttcaaatt aaggggtatt aatgaaaatt accgatgaag agttgaaaaa aattatcagt
    21181 aaattatctg gctccaaagc cgatgacatt catgatgata cggcactaat agaagattta
    21241 catttggatt ctttaaaaat tgttgagctt ttggctatcc tcagtgaaga atatcaatta
    21301 gaggtcagtg aagaagacgc gatgaatttt catacttata aagatttgta tgattttacg
    21361 caagcctgat tgttaccagt tattccattt tttgtagtga gagttggggc attttgatca
    21421 acgactgtca agttaagaag gtggcgaatt ctaatttaac aatttgaatt taaaataaaa
    21481 acagataagg tgctttttga taatttttcg attctcgctt tgtcccttat ccgttcaggc
    21541 gaggaagcgc ttgagcgctg tctcgaagcc atgttggtct tgataaaggc ttcgagacgg
    21601 cgtaaacgcc tcctcagact gaacggatag tgtaattagt tttagcttga cgcctatggg
    21661 taaaatgcct caacaaacaa tgatcaaatc agataacttc caataaagaa caggctgcaa
    21721 ttcctcctcc acctgccgcc accaaaagta ccttttcccc agattttatt ttgccatccg
    21781 acctcagtcg atccaaagcg atgcctacac tggatcctga agtatttcct gtttcttcaa
    21841 cagtttttat cgttttttct tcaggaaaac ctaattgttt tgctaccgac aaaaccagat
    21901 ttttatttcc ctgatgagga acaagccatt ggatatcaga gattgtcaga tttaattctt
    21961 ttagaaaatc tttggcgctg tctgccattc catgcactgc ttttacaaat aaagcagtac
    22021 tttcctttat ggtaatgtaa aattgctcac tatcattaca aactgcggca ggcaagcggg
    22081 aacctcctgc cggagttgag acagcatcgt atacttcacc gtcagcaaac aacgcagacg
    22141 ctataaaacg aaagtctgct tcttctttat cttgagaaac acaacatgct gctgctccat
    22201 caccgaataa aacactggtt gcaaaattgt ttttatttaa aaatttggag cgaatttcgc
    22261 tggctatgag taataccgaa ccatcattgg tctgtgctaa agcagcactt gtatgcaaac
    22321 cgacaacaaa tccggcacag gcagctccta tatcaaaagc tccggcgttt tgtaggccaa
    22381 gtctgtattg tacgagaggg ctgcttggcg gggaggggta atcccctgag atagtggcca
    22441 ggatcacttg gttcacttta tgtttttccc tgggtttttc cataaacaaa tgttctgccg
    22501 ctcgcacagc cagatcgcta caagcttgat cctcatttac ccaatgccgg tttttaattc
    22561 cagttgaaaa tccaatcacg gctggatttt gggtactgtt catccaattg agaacatctt
    22621 ggttgctcat gactctctcg ggaagcgcaa caaaagggcc ttttatatag atgggttttt
    22681 cacaacggaa aaaattcatg acaacaatcc ttctgaacgc attaaaggca gaccaccact
    22741 gactgttaca actgatcccg tcataaacga ggtttttgac aaaagtggat caatggcttc
    22801 cgcaacttga cttggttcac ccatagtgcc ctgtggaata atttgtatta ctttttcttg
    22861 ataggctgga tttttccaga attgctctgt tctggaggtt ttaattaagc ctacgcgtac
    22921 tatgttagac aagatattgt tagcactata atcaacagcc aaatttaaaa ataatccttc
    22981 cagagcagct tttgccgtac aataaacagg gtatcgactg gtgcccgctg ttgctgaaag
    23041 acttgaaatg aggactagtc gcccgaattt attttttagc aaggcaggta ataaacgatg
    23101 aataagccaa atgttgccat tcagattgtt atccaggtaa gtacgaacag cgtgataggg
    23161 caatgcatgt aatttacgca gttgggtgac ccgagtgaac gcatttaata tgatgccatc
    23221 aagtggttct cttaaaatca gttccaaatc gttttcacaa gcttgaggat ctgaaaagtt
    23281 ataaatgaaa cccgtcacgt caatgttttg ttggcggtat attgcaagtg tttcattaag
    23341 actgtcgttg ttagaacagg taatgatgat cttatcgcct gattcactgc gtcgtttcgc
    23401 tatggcttgg gcaatatcag aggagccgcc agtaatcagg atattcattt tgcccccagc
    23461 tcaatgaggt attgttcaat agtattgatc gattcgtaat gctctttctc cagtggaatg
    23521 acagggattt taatttcaaa ttcacgttcc aactcaatga ttaacaaggc cagcatcaaa
    23581 ctatccaagc cgccttgctc aagttttgcc tgtttgtttt caggaagatt ggtaagacca
    23641 attcgttcca gtacttgttc aattcgatta cttagcatta tttttttcct tattatctaa
    23701 accatcaata ttcaaaacca acgcttttct atcaagcttc ccatgttgat ttctgggtag
    23761 ggatgacatg agctgtaatt gaatgggcaa tttcctgggt agtagatgcg tttgtatttt
    23821 ttctattaaa ttttgttcag aaatggaaga gggaagcgat tttttttcga tacagataat
    23881 taatcgtgtc ccatatatct tgtcttcctg agggataaca atggcctctt ttatcacatc
    23941 aatttggcaa attttttgct cgatctcaga gaggttaact ttttctcccc caatattaac
    24001 aaggctgtct cgacggcctt tgattgcgac tagcccattg ggtaatattt ctgcaatatc
    24061 tcctgttgat aaccagccat tttgtatttt agtactatct ttttcaccaa gataacccag
    24121 catcatttga gtgccgctaa ttaataattc atgttcagca gaaagtttga tcttccaatc
    24181 gccaacagga taacctgcat aattttctgt gaataaagga tcatcactgg aataagtcag
    24241 tactctcgga gatgcttcgg ttaagccgta attgttatag atgatagcgt taggaaatat
    24301 ttgtttcaaa tctgttctga gtgcagtgga taaaggagcg ccagcagaaa caatcgcgtt
    24361 aattttggct gcgctatcag ggtatcgttt tgctatggca ttgatagcta cccagtgaga
    24421 aggtacgcca ctccacattt gaggaacggt actttgctcc attagcattt taatgtctgt
    24481 aaaatgagtg attagtcgag tggtcaggcc aaacattaaa ccagggagca attggcccag
    24541 aaggccaaaa gaataggata aaggtaaaaa gagcaattga tcttgaattt ttttaaattc
    24601 cagcgctttt atgaccgcat ggcagttggc ttttatattg atgcttgata gttgaactgc
    24661 tttaactttt cctgtgcttc ctgaggtgaa gagaataagt gctgtctggg gatggggctg
    24721 gatcgtttta ttttcacata tttcctgcaa ttctccttct tcatctatca ttacggcatt
    24781 acccaaaaga gacagtcgtc tttgcttttc ttccgctgaa atggaattgg ggaaaagggc
    24841 aatggggcga ccagtttcca aacaggctaa tagttgaata ataaatccaa tctcaggtgt
    24901 tgcatggagg acaatgatgg tttttggaag tttcaggaat tgctttgaac ttgctaaaac
    24961 ttcatgtgtt aattcatcaa aagttattac tcgattgccc tgggtaattg ctactttgtt
    25021 tggaacatct gatgccaaaa acagcatgat tttattcctc aattttattg agttgccagg
    25081 tagccactat tggcagagtt ttatccaaaa gatgttttcg gcaaaaattc ctgcgaattt
    25141 ttccgctggt ggtatgtggc attgctttaa gaggaatcag gactatagta tgtacctcca
    25201 attggtggtt ttcgtaaaca agctcaaaaa tctcattgaa taaattgtct tgagctacgt
    25261 catccatgaa tcgatttttt acttcacaca tcacagtcag tttatattca tgctcctcct
    25321 gaatcacaaa agcagcgcat ttgcccaata cgtgatggag aggagagtgc atcaggctga
    25381 actctatgtc ctgaggataa tgatttttac cataaataat aattaagtct ttaatgcgtc
    25441 cagtaacgta taactcattt tcatggagaa agcccaagtc cccggttctt aaatagattg
    25501 ggctattctc attttcttta attttccctg agaatgcatg ctctgtttct tcgggttgat
    25561 tccaatatcc tttggcgaca ctgttacttt gtacccaaat ttcaccaacc tggtcaaaat
    25621 cacatgggat caaggtatca ggatctataa ttttaactgc ttgaatggga ttaccactgc
    25681 tgactaactt gtaactgcct ggactgttat cgtcagcaaa atgtacgcga tggtcttgaa
    25741 attgttcttt ggccagagtt aatgttttgt atggacttcc tggtgttcct cccgtcacta
    25801 acaaagtagc ctcagccagg ccatagcatg gatagaaggc ttctttgcga aatccaaact
    25861 ctttaaatgc ctgataaaaa tgttccatgg tttctgctcg tactggctca gcaccgttga
    25921 aagcggtcac ccatgaactt aaatccagcc cttctttttt ttcttccctg attcgtttga
    25981 cacaataatc gtaagcgaag ttggggctgc cactaatagt ggctctgtat ttagtaatat
    26041 gttttaacca ggaaagcggg ttttgtaaaa atgagaaagg ggacatcatg attgcctgaa
    26101 ttccaccata gatgggtgtc agaatgcagc caatcaaacc catatcatga tgtgggggta
    26161 gccagctgaa aataatggtt tcatcattca tatgaaaaga ggtaaaaatt ttattcagat
    26221 tatccagtaa attatggtgg cttaccatca ctcctttagg gtgcatggtt gagccggaag
    26281 tgtattgtaa aaacgcaatg tcattgctct taatggaggt tggttgccaa ctactacttc
    26341 tgtttaactc aatgctctca agcgcaatag caggaatttt caggaatttg ggatttgtat
    26401 ttaattcgtc tgcggtgaat tttttgatgt gatccgcaat catcagtact atgaccggtt
    26461 ttgagttggt aactatgcgt tgtgccttgt ccaataattt ttcttgggct ggtgggtaaa
    26521 tgggtacagc aatgcaacct gcgtaaagac agcccaaaaa ggcctggata aggggcaatc
    26581 caggcgcaaa taacagcaat accctgtcac caggttttgc tccttccgct tgcaaagttg
    26641 ccgcaatagc tttggcgtgt tgatccagtt gctcataagt cattatctct tctaactctt
    26701 tgttcagaaa agtacagctt ctcttcttag ggctgtgtaa agcacgtaac cttacgacat
    26761 caaccaggga ctggcactgc aaatattctt ttttcactat aaaacacctt aattgctaaa
    26821 agaggctttt tgatagttta gtacttgtcc tgcataggac agaccaccac caacactcat
    26881 caggagaatt tttcctgatc tgtcaaacaa tttttcctcc actgcccttg ctaatgtata
    26941 gagaatggat gcgccgccta tattgcctat ggtatcagca tcccaaatta ttatcttatt
    27001 gtttaaatga ttttcatgaa gcacttggtc tattaatttt cgattgacct gatgtggaat
    27061 tatccatgtg atctcatttt tttccttttc tgttggcaat aagttatcaa gcatttgcat
    27121 gtatctggaa taggctaatt ctttcatcag ggtttcatca cccacaagat gataaccaac
    27181 cgaatcaagt tgcttatgag ttggcggaaa taccccttga gtcgtaaatg cgtcgacgta
    27241 ttgaccatca gtagaaaaaa aactcttttc aatggtaaaa gacccctcct tatttttttc
    27301 caaaagtaag gaggctgccc catcagccaa acctatccag gttttttcat tttcagggtc
    27361 aatcactttg gaaagcgttt cagagcaaca aaccaaaatt ttttgatagc ccaaagccat
    27421 caatgcgtaa gcaaaatgca aggttgataa agaggtagag caaccggttt ttaaatcgta
    27481 agcaggcgca tttaatccca gacttccgag tacagcagcc gcttgggagc ctgtaaagcg
    27541 tttattggtt gtggagccta agagaaatgc gtctatgtct tccggtttat aatgcgcaat
    27601 aagcttttct actgcttgaa tagccaatga ctcagaactg agctctcttg aattatcgag
    27661 tgattcccaa ggtttgtggc accagtagcg agtatgaaag ccaaattctt cacctatttt
    27721 ttctgcgaat ttttcgagaa aaccttgaga tttatcggca gttctcggaa aattttttaa
    27781 aatttcaaaa tttgtgatag ggcctgatgc aggcagaatc cttgatgcgc cagtaatttc
    27841 aaattgtatg ttaggtttta agatattcat aaagtggcct tattttgtct ggagcgcata
    27901 gaggttacaa ttattgaaat tacactcttt ctatcctgcc agatcaagaa tttttatctt
    27961 tttcgtataa atagctctaa acttatacaa ttaagagtag cctattttgg gaattaagtt
    28021 attatgagta ttgatcaaat ttccaagtta caaagtctca gattccaaga tttaaatatt
    28081 gcaagaggaa agaagtatgt cttcccaaca tacagataag acgttaataa aatggataga
    28141 taatttaatt gatgaacaaa gctttttacc aataggatct gaatatattc cagataataa
    28201 acctcattcc cttcctggcg ctgaggtgat tacgggatta gccaaagtga atcaaaggcc
    28261 agtagcgatt tatgctcatg attcttcagt caacaggggg tatgttaccc gacaaggcgc
    28321 tagaaagatt attcatttaa tggataaagc ggaaaaatta cgcattccta tcgttgcttt
    28381 tttagcatct cccggggttt ctctcgaaga aaatttgctg agtggcgacg aatacactca
    28441 aattattgct cgaaatatta ggctgtcagg aattatccca caatttgcag tgatactggc
    28501 acccactctt ggagcgccgg catattccgc tgtgttaatg gacttattat tttttaacaa
    28561 gcatagaagc tatcttatgg tgactagccc tacggtagta caacaagcca ttggtgaaaa
    28621 agtgactatg ggcgagctgg ggagcgcaac attgcatgca acgacaacgg gtattgctga
    28681 tttcgtggac gatagtgtta ccgcacaaat aaaccatgtg aaagcaatac ttggtttttt
    28741 tcctctacat accaatgaac gtccgcctgt tcatcctatc atggagccta atcaccccat
    28801 tcctgatatt cctgcaaatc cgagaatccc atttaatatg ctcaatttaa ttcaggctgt
    28861 cgtggataat tcaaacgtta tccagtacaa aaggacttat ggacaggcga tgatttgcgc
    28921 ttttgtgcat atacacggct ttgctgttgg ggttgtcgct aatcaaagca ttcgttacag
    28981 cggagccatc gattccgatg cggctcaaaa agcagccaaa tttataagaa tatgtgatgc
    29041 ttattctata ccaattttga cttttatcga tgttcccggt tttatgcccg gtaaaagaga
    29101 ggagcaaaag ggattattgc agcatggtgc aaggttttgc gtagctatgc aaaccagagt
    29161 gccaaggcta agtgtcatcg tgcgtaaatg ttatggagcg gcagcttttc tcatgatgca
    29221 aaccaaatca cagaatggag atttggtttt ggctctggaa acagcaaatc ttggtgtcat
    29281 gggaaaggaa acaactaata aggtgcaagc catggatggt caaccagtac aggaatcatc
    29341 acaatcccaa ttgacagaaa agtctcaaat tgatgattcc ctcatggaag cttattcatt
    29401 gggcttgatt gatgaaatta tccaggcagc gcaaattcgc agtcgccttg cctctcattt
    29461 ggagttttta tatagaagaa tggaaaccat gcccccggca aaacactcta ttgtataagg
    29521 aaaataatga cctcttatga aaatttgata caactgtctg aattttttga gtcatgcctg
    29581 ggtaatcctc tggaagacaa ttcccctgtt aattttcaac aaattttgct cgctgatgag
    29641 caggaaagat tagcttcgtc accaatagaa tttattaaac aatgggggta tttggactat
    29701 accgtacctc gttatttagg gggtaaactg agctccctgg atgagctata tactttaacc
    29761 aggttgttgg ccaggcgtga catcacgatg gctattatgt ttggattggc tttctttgct
    29821 gcattgccaa tatggatagc tggaaactca aagcaaaaga aacatttgag tgagagcctt
    29881 cgccagggaa agataggtgc tttggctttg accgaagaag agcatggaac cgatttgact
    29941 tccaataaga tgaatgccag gccaactgaa gatggctggg aaatttcggg acgaaaatgg
    30001 tgtgttaatt ttgcaacctt gagcgagtac gctgtggtgt tgtgtcgtac taatgaaaaa
    30061 ggcgggttgt taggtttttc tttgttttat atcgataaga aatcagccga aaagggtatt
    30121 tctcctacac ccaagctgcc gactcatgga gtcagagggt tagatatcag tggtttttct
    30181 tttgataaag tgaaagtatc taaagacgca ttaataggga aggaacaacg tggtttggaa
    30241 ataacctata aggtattcca gatttcgcga accttatgcg cctgtttggc tattgggggc
    30301 gctgatactg ctttgaggct ggcattatca tacagcttgc aaagacaact gtatggcaaa
    30361 tcagtgtttg aaatacccgc agtaaaacaa cggcttggcg aattgtttac tcttttatta
    30421 atcgcggatt gcactgctca ggttatggtt cgtgcctgta cggtgatacc ggaaaaaatg
    30481 agtctttggt cagcgattat taaattcctt attccgcata ttggagaaga tatagcagaa
    30541 cagtgtgcca tcattttagg tgccagagct tatctgagaa ccacgcaatg ggctattttt
    30601 cagaaaataa gacgcgacat tcaagtggtt ggcttgtttg atggaagtac tcaagttaat
    30661 ttatcactta ttgctggtaa tttgttgcct caggcaggca tgcgaggtaa acattgggca
    30721 gagcactcgg aaaaaattga gaaaattttt aatgttcgtt tgtcttgtcc tccttttaag
    30781 gaggatagac tcggtttgtt tactcatgaa gaagatgatg tattggcggg tatcctgtcg
    30841 ttaaactcaa ttccaatcaa tccattgatc actacaatac gtcatgaaat tgaaaaattg
    30901 gatcaagaag tcatccgttt acatgatgag aaattatttg acccacgcag tttagccgtt
    30961 tttcgtttgg cagaaaaata ttgctggatt tttgccgcca gttgttgttt gcagttctgg
    31021 cactataatc aggagatact ttctgatgat ttgcaaaata cagattggct ggatttagct
    31081 atgcagttaa ttcttaagcg attgaattca ggttcgatga ttgatagcgt gttacaagaa
    31141 gctatgagtg agcgtttgta ttctttttat caggaaaaac agctgttttc tattatgcca
    31201 atacatattg cggggtaatg agggatgaat tggaaaagat ttttaattgc cgccatgaca
    31261 aagaaaaaat accaggtttt tctgaatgat accaagcatc ctaaagaggc gagagaacga
    31321 ttatggaatt cagaaattgc tccattgttg gaaaaatcat cttattggca gaaactatta
    31381 aaagatcaat ctcctgtagt tcttaatgat tttggaatta cagaatatga agattatcag
    31441 gaaggattgt tggcagccca gcattctact atccaacctt ttaatggtga agaattgatt
    31501 ttttggtcag aaacttcagg aacatcagga gttagaaaat ttttccctat aacagattca
    31561 ttccagaagc aatttcagcg tacaatggcc ccgtatattt acagtctaac ccagcgtttt
    31621 cccggctttt tcaaggaaaa aatgttgtat cttgtagcgg tagatactca taagacgact
    31681 cctgccggta ttcctacggg atggattagt aattttaatt atcgccattt gccttccttt
    31741 attaaaagat tttatgccat gcctgatgag gtctttgcca gccatgagat ttacgaacaa
    31801 tggggacctg tctacggttt ggcttctgat ttaagcgctg tatttgctgt gacgcctatg
    31861 gtaatcgatg ctttttataa gcgctgtatc gacggtttta aagactatct tcctcattta
    31921 ttggatgata aacctcttcc atcacatctt ccacctctca aaatgacccg ggaacgacga
    31981 agacatttgc aggctttagc caaggctgac aatctctcat tcaaacaact gtggccatct
    32041 ctggaaatgg ctggttgttg gacatccggg ctttgcgaat atcctgctca gcaattacaa
    32101 aaattgctgg gtcctgagat taaattggta gatggcactt attccgcaac cgagggttgg
    32161 ctaacagttc cgatagaaac aaatagcgtc ggtggaattt tacatcccgg tgcccatatc
    32221 gtggagttta ttccagaggg agcggcgatt gagaaagaga atcttttaca atgttgggag
    32281 ttggaagtag ggaaaaaata cgaagtattt ttgacaacag ctatgggatt tattcgctac
    32341 agactcaaag atatcgtgaa atgtaccggt tatttgaaca gctcaccaaa acttgagttt
    32401 tgttataaaa cgcaactctt gaaattggaa acatgctcaa ttaccggtaa agaattgcaa
    32461 agcgcagtgc tggcaacgca tcttgatata gagccttatt ggtattttgc gcgcaatagt
    32521 gtggggaatc gtattgtttt ggtgactgat gatgaggcag agatttcaga gctatcactg
    32581 aaaaaattgc atgaacaatt aattcatatt agtcctactt atgctcatag tgtggaaacc
    32641 gaagaagttt tacctgtgac cttgctgcaa ttgcccaagg aaaaattatt agctggcatg
    32701 catgggcaaa ccaaaccgaa atttatctct caggaggtta ttgctgagag atgaagcatc
    32761 cgctgacaaa ttcattttat cttagccaag tagaaactga aattaaacag cttttcaatg
    32821 atgatagcga gtatgaggaa tttctaaatc ataagatcga ttatgaaatt aactcctttc
    32881 tggccagttt tattggagag tccttgcttc atgggtgtgt aggtcaccat gcgtatataa
    32941 gattaatatt aaaaatcgtg agtatttaat agaatttacc cctcagccaa ccgataccaa
    33001 agaagctcca gctcaaagcg aaacaagaac aataagtggt aaaaagattg tagaaatgct
    33061 ggcctatcat aaaaaacttc aggaaacgaa tccaccgaca gcgtctttca tattaactaa
    33121 aatgaaaccc ggtgataatg actgtttcag ttacgtcaat aaaatactta ttggaacaaa
    33181 tcaacagggc gctacaatca agcgatttgc caatatgggg ctcattggtt ctttattagg
    33241 aatggggata gaggctttaa gtccttttaa accagatttt tttcaaacta caagttcaat
    33301 gagaactcat aaggaaaaag tgcctggcca ttcgaatgat ttgtcaatga atagcctgct
    33361 acctcaataa ccagccctcc aatatcggat tgcaaagata atctgtcttt ttcatgttat
    33421 tgccactttg tgcgtgttta ctgcatataa agtccgctcc tcaaaacgtt tgtacctttc
    33481 cctaaaacaa aacaatcacg ttctgcaaga gttataatat cttgatgaaa gatataaaaa
    33541 aaacaaaatg aaaaggtatt ttatacattg ttgaatttat gtcatatact ttaaagcgat
    33601 agctcttaaa aataaggtat atatcatgga catgaaaaaa tatattttat ctctttctct
    33661 actgacggtg attaactctt cagctatcgc tacgactcag gaaggtgagg aggcctataa
    33721 caaggggtat tacgaaaaag catttatttt gttgtctcct gaagcggata aaggagatgc
    33781 caaggctcaa tatttgctgg gtaagatgta ttataatgcc caaggtgtaa cttataatcc
    33841 ggaaaaaaca gagcaattat tacttgcctc agcgaatcag ggcaatgtcg atgcccaggt
    33901 attgttagcc ggtttctatt ggtaccttaa tacaccagag ggctataaaa aggcgtttga
    33961 atggtatcag aaagcagcgg atcaaaataa tgcggatggt caatatggct tgggctatat
    34021 gtatgataca ggaactggtg tacctcagaa ttccgataca gccatggtat ggtataaaaa
    34081 agcagcagag caaggcaact ctaatgcggc tttagctatt ggttataatt atgataccgg
    34141 tacaggggtg aaaaaggata agactcaggc tcttaactgg tatgcaaaag ccgctgactt
    34201 aggtaatgcc agcgcacaat ataaccttgg tttgatgtat gagcaagggg atggtgttcc
    34261 taaagatttt cagaaggctg ctgagtattt cgagaaagca gcaaatcagg atcatgccaa
    34321 atcgcaattg gagttaggtt atttatatga ttcaggaaaa ttaggtaaat cggatctgca
    34381 aaaggctgct ttttggtatc aaaaaagcgc agatctgggg aatgctaatg cgcaatttaa
    34441 tttagctgat atgtattttt acggtgatgg ggtcggtaaa agccttgagc aatccgtata
    34501 ctggatgcaa aaagcagctg agcaagggta tggtaaggct caaaatcaat taggtatcta
    34561 ttacagagac ggtattggtg ttgctgctga tcctgtaaaa gcttatgcct ggtttacagc
    34621 agccaagagt aatggctttg aaaaagcggc gagcaatgcc agtgatcttg agaaatctat
    34681 gaacccagag gatctgagta aggcgagaac tttaggacaa caatatacgg ataattacaa
    34741 ggccaaaaaa taaatgcctg ttacctgttt tcaagagcta gagatacggc atttgtgggc
    34801 tatgggagtg tgcgagagaa ctctcgcata cactcgggtt cggcagccca aaaatagaaa
    34861 aatataaaat taatttcacc agaataaaga gacctctttg aatttaagaa tgtatcttcc
    34921 aatcttttgc ttgtttttgt tagtatacct ccaggtttaa ataaaacaac ctcaaaggtt
    34981 ggcaaatgaa ttgtctatta ttttcaagga aatcacagga tgacggagcg caagaaaacg
    35041 acagtcaaga ataaaaaagc acctgtcaaa aaaagtcagc tgactgtttc tttattgatt
    35101 tttattgatt tgatttctat attgctcagt ttaatgcctg gcgtggctgc atttagctta
    35161 accggtttgt ttttgtatta tgggttttca aaaacctggc ttgttttaat ttgctcaccg
    35221 tttgtattac ttttttcttt tattcttatc attgcactta tcaggataag ccttccaaaa
    35281 cttaagccag gtcgctataa acgggagtta aataagacca tgatcgcctg gttttgtcat
    35341 tttgcactct ctcgtgcagc gaaaattgtt ggtctgattc atttattaca atcctttaat
    35401 gttattaaat acttgtattg gcgtgctttg ggaattaagg tgtcatttcg cgttatgaac
    35461 tcatttgata tcgattttgt ggattgccct ctcatttcaa ttggaaagga tgttaccctt
    35521 gccagtcaag tctctatcag ttgtcattct gatgtaggaa attatctttt tttatcgcct
    35581 gtggtgatag aggataatgt ttttgttgga atgacgacta aaattggccc aggaacaaca
    35641 attaaaaaag gggcctggat aggttatggg aatgcttttg ttaaccaggt tattgaagaa
    35701 aatgcaaaaa ttggcaatgt taggcctgtt cctgaataga atgaaatgtt ttatcttaag
    35761 tttggattaa taaaaatcat agtgccttat tttcccggtt aacttaatga atccagattg
    35821 ctgcaatcag gctttaaagc cgctgattat ttctttgatt aaacggatgt tccagaatga
    35881 aaaaaattgt ttacgtcttg ttatttattt cttcgctgac tcatgccaat tgtgtgatct
    35941 attttaacga acagccgctt aataaaaggt tatctgatcc tttgtattct ctgttgtcca
    36001 atactgaaat ttgcccgcaa tcgattcagg agttaagaaa ttccatgaga ttgagaggat
    36061 taaaagaagt catagggatg gtcgctaatc gaggaagaaa taatcctgct gaaggcagtt
    36121 tcagtttttt tgaatcagta tatggtcgat tagcaactga ccaattgatt gcaaaggggg
    36181 agttatttct ggggtacttt actcgtctaa aagatggcct aattattttg gatcaaaaac
    36241 ccaggaaagg taaattattg attgaagcaa tcagctggga tgctaaaaag gcgctttata
    36301 atttttatga attgcgagga atagaacatg gaggcgtacg ttggttttat cgaggaaact
    36361 cgagggatgc ttataaagat aacacctggt tatatagaat aagtccagag ggcgaaaacc
    36421 attttggcag tcgtatgcga tgttcggctt gccacaattc cggcgggccg atccttaagg
    36481 agcagtctct tcctcataat gattggtgga ctaatcaacg tccacttatt ttgctcccca
    36541 ataagcccga tggcgatgtg agccaattat tggaacaaat cagtgatgtc agtgtatttg
    36601 caaatgacgt gataacagga atgcgacatt tgagcgatag taaaaaaatg aatgcatttc
    36661 aagggaaact gtcattgcag gagcagttac ggcctttatt ttgtactacc gaaataaatc
    36721 ttcagtccag cactgactca tcagaaaccg ttgtatccat tccatcagcg ttttggctta
    36781 atccactatt aggcgacatt catttaacca tttcaatgga agattataat caactactga
    36841 aagagtttgg cactcgtttc ccagaaacgt ctttgcaaga cgctgatcat gcctggctta
    36901 caccggtaaa gggggaggct gatctcaacg cgattaagca acttatccaa cacaatataa
    36961 ttagcaaaaa atttgcacaa tccgtgctga tgatcgattt taccaatcct ttgttttcat
    37021 caaaacgatg tgagttatta aaattaatac cggaatccta tcaaacgaac agcaaagaat
    37081 gggttgaaca atttctgatt aatttaagaa acagtgagga gcaatttaaa ggcgctcgtc
    37141 agctagctga ttatatgagt aaagagcaat tggattcaaa agagctaatg aaaaaaatca
    37201 aaatttatga atactttctt aatcagtcta tatcaaccag gtcaggatta agtaaacatt
    37261 ttcagcattt aatcgagctg cgaaaagcgg tctttcgtag tgaactgtca caaaacccct
    37321 tagggcaaat tttagaacca gggtttcggg tgattttccc tgaaagtcag cagtagacaa
    37381 gaagaggtct attattggct cagtgcatga caaaaaactg ccatgcactg agtattattt
    37441 tctacgaaag atttgaggtg atgaaattga ttgcatttcc tccaccaatt gtgaactcaa
    37501 caggaatata gggcagatta cagagaaaca gggtgacaat gaaaaatatt gctgtaccta
    37561 tattccccat ataaatccat tgcttttcct ggttttcatt caaaaatgct gtaatggcat
    37621 aagtgaaact gaatagtacg gcaaccagta agtaaatgaa agcatttgta tctagtgaga
    37681 agtcagaaag gatcttataa gtcatcatta aagccagaag gaacagaatg actcgataga
    37741 ctcttaaaga taattttaac caggaaggat agtctgattt aatagttcca tcctgaaaat
    37801 aggcattgaa aaatatgata cctgctgtag tcagtggaat tagtactatt aacgggttga
    37861 tgtgttcctg accgctcgaa aaagaatgaa atgtatataa tataaaatag aggatactta
    37921 ttgctgccaa gaggggaaac aggtaataca ttattctaag taataaaaat cgcatgttat
    37981 gaatgatatt gagattttgt tgaccaacac ccaaacccat aaaaaataag gttgtattac
    38041 tgattaattt aaaatcacga ttataaaaat acaaatccca taaataatta tttccaacgg
    38101 ttttaaatac aaaagatccc aatacaataa gtaaattggc caatgaggaa aagacgaggg
    38161 caataaacaa aagaggaatt gtattccata ctccagcaaa taaactgctg taacttaccc
    38221 gccaggtgtt gtcatgatga taagcgtaat ggaaacaatg agcgacgtag ataaaaaatg
    38281 gaaagccaac aaaaaaagta aaaaaatgga aatagtcttt cattgagtag tccattttca
    38341 acggaaacaa tggtattgat agaaagaaag cgagcaagaa gctggttcca atcagacgcg
    38401 aaaaattgac gttgttataa gtaagggcaa ataaaacacc aaagatagtg acaacactgt
    38461 atataaatgc agtcgtgttg ttatagcgta tcagtatgtc cactactacc cctagaagta
    38521 atccaatcag ggtaaaaata taaaggtgtc tttgagcagc catcctgtct ttctccacaa
    38581 gggggttata ttcatgattt agggtttggt agaatgccct aatcgttatc taaaaataat
    38641 tctaactcag ttacttaatt gatctaaatc gtttattaaa aatatttttt cgttccaagt
    38701 ctgagcatgt gacagatcta atttcaatca ttaagaaact cacaaagtag tctttgaaaa
    38761 tgatgagtgc cttgttcttt tgttggggag aaacgtccca tatcataata gcgagatttt
    38821 atcccccttt gcacagactc caccactctt tcatcttctt gttcaacctg gtcaagtgct
    38881 ccaccagcgc cttccccaag ttttgattca tcaagaacaa aagataaaaa ggaaacctga
    38941 gttaactcag gactaagcgg tttgactaca ttcacagaac aaccccaggg ataaaaatta
    39001 agcatcgtat tggggaatat ccaaaagtaa taggcagcaa cctgtttgcc ataatcttgc
    39061 gcccctttgg gtaaattaaa actgacttca tcagggcttg ccaaagccaa ttgcagatta
    39121 cagtagcgga agagctctgt ggtgtaggtg ttgcaatcga ttacttttct tagcgaggga
    39181 tgtacaaaag ggatatgtaa cgcttcaagg taattttcac agtataaggc ccaatgcgat
    39241 ttaactacat aatctttgca cttttcttta tccaggcgca ttttttccat aggcagccaa
    39301 aacaatcttt ctttaatttc tgcaaatact tcatggaatg ggacgatggg ggaaagtgaa
    39361 gcaaagagaa aaggttcaag aatacccgtt gaaatttgac ttaggttatc attgctcgat
    39421 gggaaatctt ttgtgagttc aaattcaggc atatgaatta attcgccaca aaaattaaac
    39481 cgacggccat gataagagca cttgatttta tgagctacgc aaggttcctc gatcaggatt
    39541 ttacctcgat gggtacatac attactcaaa caacgaattg tacccgattc atctttcgta
    39601 aacagcaagg gttcttcaag aaatccaggc agcagggtaa aaggaattaa ttgtctattg
    39661 gttgtcagct cttcggtact gatacaaaat tgccaggttt tactaaatat cttttctttt
    39721 gtagtttcaa accattctgt tgatgtataa aattcagaag agattgtgga ggcttttgta
    39781 atatcaggat ctatatagag attcttcatg aacatttcct tactgtaaag agaaaaacct
    39841 gcaaaaagag aataaacatt ttaaccattt ctttggatga agcgatggtt aaggtttggt
    39901 aaaaggtagt gcaaaccttt gtaccctgtc aacttgttta tttgataaca aaatgattga
    39961 ttaattatcc taatgatgca aaaataaaca cagtttgggt tttaaggaac tattgaatga
    40021 gatctagccg gtttttatgc ttattaaatg gcatgttatt atccagtaat gttgcagcgt
    40081 tttcaccctc tgcccctata ttcagccctt atgttgattt aacgattaat actcattggg
    40141 actcgcaatc acaggatatg gagccaatgg atttgatttc tccggcacaa aaactgggga
    40201 ttaaagctta tcatttggct tttattacag acagtggtca atgccaacct gcctggggag
    40261 cccagcaaaa ttacagtgtg gcaaagggat ggggtaaaaa acagttcgat acgctatcaa
    40321 gagagggggt gaaactcacc gtttcatttg gcggagcgag tggtactgat atttcctatc
    40381 attgtgataa aaatcaattg attaatacgt ttaatcaagt ggctaatcaa tatcatgcga
    40441 atgccctgga ttttgatata gaaaatggaa cagccaatat tcctaatttg ttgcaggctt
    40501 tgaaattatt ccaaaaggag catccggatg ttttgttaag ttttaccctt cctgtgatgc
    40561 ctgagggttt aacttctgtt ggaaaagaga taatcacctc agcggctact ctaggccttc
    40621 attttaatgt gaacataatg gctatggatt acgggccggc ttatagtggg gatatggggg
    40681 attatgcgat atccgcagca acgaatctcc atcaatttct gcaggaaatt tatccggata
    40741 aaaaaccgga ggctttatgg cagatgattg aagtaacgcc aatgataggt gtgaatgatg
    40801 tgaatacaga gcaatttacc ttatctaatg cagcccaatt aaaacaattt gcacagaaaa
    40861 acctgctggg gggattgtca atgtggtctt tcaatcggga taaaccttgt gctgacaaat
    40921 gggcaagtcc aatatgtagc ggtaataatt tgcagagtca tgattatgag tttgtgaaat
    40981 attttcagta atggaattaa ttcaggattt cctggttctg aagaagaaat gatttgaata
    41041 aattatctat gttatttttt tgtcaagata aacctggttt tgatgaatta ggtgatataa
    41101 ttttcactat gataaattct acaaaattca ttacttaata tttagataat aatagatgca
    41161 tatactggaa ttaagattta gcgggtattt ctccatttat tgtttggaag agtgctaact
    41221 ggcaattagg gttaattatg aagagtaaag aagaacgatt taatgaatgg cgccaagaaa
    41281 aggcgaaatc agaacaggca tatattaaaa ctgttccatt agtggaagaa tacattaaat
    41341 catttgatac cctggctgag ctcagaaatg atgtagctaa tcctaattct gaaaaaatta
    41401 aaaaaatgga cgcccataaa aggcgcactc tgggtgtttt actctatgcc aaattaaaac
    41461 tggatgaagt gatagaaaaa atttcggatt ctgatagcca ggaaaaggat ttattaattg
    41521 agttaaataa tactctgagc aaagcgtatg ataatcccaa atcggacgcc aaaaacagag
    41581 taaaaatttt aatagaaacg acagaacact tattgagtat cgaacatcac tcttatccac
    41641 aattggctaa agaattatgc acagatctat atgctctatt atgtattgcg aagagatcac
    41701 ttaagaaaaa tgaaatactg gcaggctggt tcatcgatgt tcatgaaatt aataaaaaac
    41761 tggaagaatt attagcaaaa acagaagaaa aagtcgaaat tttggacaaa atcctgtcag
    41821 aaaaactcaa tgaatcactt gagacttccc aaaaagatag aacgatacaa gaacatttta
    41881 atgagcgatt caccgaaatt ttaagtgggg acgagaaaca aagaacaaaa ctggataagt
    41941 taaaaatgag ccttaaggaa gtagctgagg gactatcagg tattgtggtg ttaagaaaag
    42001 agaaggttac gattggtgaa aaaattaaaa aaattgaagc tttactgaaa gcagtagaag
    42061 aaaacgataa aaaagttaca ggaagacaat attttttaga cttactagcc agccataaag
    42121 accattttaa tactctaatg agtactgagg gtggggagtt aaaaaaacag ttagcgggga
    42181 aaatagaaca actcaagaac ccggattttt atcaaactgt ttcttcgacc cttttatggg
    42241 gcgcaagctg ggctacttct ttggctacag tcatttatcg ccagataact cctcaagcag
    42301 ttcaggatag agttgtcgct tatgctcctg caacactgga tagtgaagca aaagctcttt
    42361 tgcaggaatt ggcaagacaa cagttaattg agttaaaaaa acaacagagt ctcaaaaggg
    42421 atgaattgat tcagagatat agccaaatat caagcggtag ccctgagttg gaaaaactga
    42481 taatcaacgc cacttcagac catcttgagc aaattgctgc agcaacaaca gaaacaagca
    42541 aggttcttga tggatatgat caaattgaga ttaaactcaa agataatgta gaaaaactta
    42601 agggaaatca gttaactcga aagcaattat ctgtttttat tgaaaaaaat gacacctgga
    42661 tagtaaaatt atccaactgg cttgcagaaa atatccatga gatatttaaa tctgatacgg
    42721 ctcgcatgat tgataaggct agaaaagtac aacaaaagct gggagaattt caaagacaat
    42781 atcaaacgga aattgaaagt gaaaaagcaa aaattgaaaa taatgattca ttgtcctcag
    42841 agatgaaagt ttttttaaaa aatcagtttg aagaaacaaa aaatgcaggt ggaatggagc
    42901 agaaaccaca gaaaaatctc agcaaggaaa ctcttcagaa attatttgtt agcctgttgc
    42961 ctgaaaatga agagatctca tctggggtaa tattcagtcc gtttaattaa tcagggatta
    43021 gagcgatgat atgagtcaat cggttgatca aagtcaagtc attgatcgat taaatcggta
    43081 tctcaaatgg cataatatgc cagttagcat gaatgaagaa ggaatttgca atggcttggt
    43141 taccgtgtac gcaaaatacc tactcgaagg taaagaaaag cgcttttttg aattgcttga
    43201 tcagatagtc agaatggaag aagactcagc aatagagtct gaggtaaatc agtttgtcta
    43261 tgatgtcgtt ctcactttat tcccgcatca atttaataaa aaactaagtc aggctaattc
    43321 catgtgtgcc ttgacaataa acaataaacc cttggtttct tcttttgatt ttgcaattgc
    43381 aactcaagat aagaattggg aggaaatctt taaaaatctt gctctgcaag aggatgagat
    43441 tgctcgtgtt agcggaacag cgcatgccgt agccgtcagg aaaaaaaata ataaatacat
    43501 tgtatacgat cctaattact cgtctggtac taaggagttc tccaatgaac acgaattgat
    43561 ttccgagtta cattacaatg tattcgggta tcataatgga ttttatatta agggtggtct
    43621 tttggggatg atggttagtg tcatccgcca tcccgacaat aagaagccaa gagcttttcc
    43681 tcaagtgact cagctttatg atcgttattt aaccgaaaac aatattaatg acgtcgctgt
    43741 ttcatttcat ggtggaacat ttaatacttt agagcatgct gcaatggtta gtcatgcaga
    43801 agtaataaaa aatttgcttg agaaaggagc aaaagataat gatcatcagg ccgctggaaa
    43861 tgctgtcatg tacaacaatg tagaagcatt aaaagcttta ctgggaaaaa atagagagac
    43921 agaactcttt caaaggctat ttcacgcggc tttggagagc gggagagaag aagtttatga
    43981 tgccttgttt tccatgaaag gtgccttgcc atttaattcg tctgttcaag ccattcgggc
    44041 tgcagcaaaa gggggtaatt ctgttctctt ggccaaggta atgaattatt ttaaaagaaa
    44101 tcatactgga ttttcagatc ttgaagacgc tattccttct gcaattgcca gtggaagtgt
    44161 ccaatgtgtc aaattacttg ttaaccaatt aaatgaaagt aaaaaacctt tagatgataa
    44221 aaaaaggatg gagtatttgc ttgagtcaat aaagcaaaat caatcttata tggtggctta
    44281 ttttcttaaa gatatcccac aggaacattt aaaaacaatc tatatgagtg tctctgctgt
    44341 tgagagaaca aatctgcata ttttacgtca gttacagaat tatggagttc cattttcaga
    44401 tatgtcaaag gctatttttg aacaaaaaga gcaccatttt gtaggtttaa gacggatact
    44461 aggtgttttt ctgtgtaaat tcactgattt tcttaaagaa ctcttattca atgattgcgg
    44521 aatacattgc catcgagcag atgagagtgg agtaaaaaaa tcattttttg ccttccctaa
    44581 aaaactggtt ggagttgaac aattacaaat ccccaaactt tattgaggca cggtactaat
    44641 gacaaacagt agtcatcagt ttatattaat gaaataagat ggtctgaaag gagcttgatt
    44701 cattgtgaat atcttattgg caggaggctc tggattaata ggacaagagt tagttcatgg
    44761 aatgggagag gagcaccatt ttactgtgct ggggcgtgat aaggacgcaa tacaaaagat
    44821 ttttacaaca aaagcatcct ctttgtcctg ggatgaatta aatacccagg atgccagtca
    44881 gtttgacgct gtaattaatt tatgtggggt taatattgcc atttcacgat ggacaccaaa
    44941 aataaaagaa gaaataatca gttcgagagt gcaaacaaca gaaaatttgc ttcattggat
    45001 tttgcagcag aatgccaaac ctcgctttta ttgcgccaat gcagttggga tttatggttt
    45061 acaggaaaat ggtgacgcaa ctgatttgac tgaagacaca gcaattaatt gggaacaccc
    45121 aaaggattac ctaagtgaag tgggaattcg ttggcaacaa gccttacaaa aggcagagga
    45181 ggcgggcctt cctgtgactt ccacacgctt tggcgttgtt ctaaaaaagg ggaaggggtt
    45241 tttagggaag ctctatccca gttttatttg cggattagga agtactattg gggatggtcg
    45301 ccaatatctt tcctggatag acagtgagga tttggtcaat gcctatcggt ttttgttaaa
    45361 tcatcctgaa attaccgggc ctgtgaatgt gacctcacca aatccatgca tgcaaagaga
    45421 atttgctaaa gcctttgcca agtctctgca taggccacta ttattgtgga ctcctgcttt
    45481 tataattcat cttttatttg gtgaaatggg tgattgttta atcaatcacg ggcaaaaggt
    45541 aaaacccaaa cgacttttgg aagctggttt tgagtttata tggcctgaca ttgaaaaggc
    45601 attaaagcac cagtattctg ctatacagta acagcatttt taagaggatt tcatgatttg
    45661 gagggatatt taaaataccc tgtaagttac tgaatacttg acagggtatt taaaggagta
    45721 ctaattaacg agcactctta aagtattgtt ttaatttcat tggagaaatt tgagagccat
    45781 ctttgatagc agcggcgatt aaattaggag tgcctgtgtc ataactgcaa atgatgtctt
    45841 cgtcttgttg atgaggaact ccaggagcat tcatcatgct taacacttca tttgatgctt
    45901 ccaaagcgcc ttcctcggaa tctgcttcaa ttggagcaat gaaaaagccc catacagaag
    45961 aggtgctgta atcgctgata ttataagcca gatagtaatt catgccaatt tcttcagcca
    46021 taggcaaacc ttccgccttg attacattga tatctgggca agcaccttgt tgagaagcaa
    46081 aagcagaacc ggcgagaatt aaagcgccaa aaaaagtcgc taatttagat tgaaacatca
    46141 tgttttttac tcctgagttg tttttaattc cgcgcaaaga gtatcatttg cctagaagat
    46201 ttacaataat gttgtaattt gagattggag ggtaaacgga aaacaaactg gccttattct
    46261 cggagctatg atcattgatt actaatttta tcgtctagaa ttagtaatca acgcagtaag
    46321 gtaaatcgct aatcgttttt gaaatcaata gtaactattt catggccaaa acagttcgcg
    46381 ccatcattac taaaattaaa atttgaaata ataaccatta acacgacata ttatgcgagt
    46441 tggaatcttg aggtagcaat tgtttctcaa tgacctgcaa cagcagagta agttctcttt
    46501 catacatggt tctgtcatat ccaccttcca agccccaata gatggtgtca atgcagtctt
    46561 tgttctcgtt atacaaagtg aatatggatt catagaagta gcttagatca ccgtcattaa
    46621 atcgaaattg ctgagcggca gttttcccca gcatccggcc tttaacaaat ttaccgcaat
    46681 acgctgtttc atcttcatga gaatcccatc cggttgggag gtataaggca attttttgcc
    46741 cttttgcttt cgcctccctg atcgattctt ttagttgttc taaagcaaaa ataagagcag
    46801 gatgtacgcc tactgatttt ctggatgtta aactcagatc cacggcataa tagtttaaat
    46861 tattgtggcg ccagacgtgg atatttttcc ctacatctac accttcagag ttgaattcgt
    46921 tattaatgta ggcaaaatct tgttgcggat atactcttga atcaaacaca tcaatgtgac
    46981 aaatcgatag atgggaaaat gagtgtctta aaatgtcaca taggccattg tcgcgattga
    47041 cgtcggttcc tataatgaca aatctaggtg gttgaacaga actcaactca gcctgtttta
    47101 tcagcatggc tgttttattg ataaggcaaa aaccgcttcc catttgggta taggcatgat
    47161 ggcttggtaa accaaaagaa aaatgggttt ttgccggatt gaacagagtt gttgcacaat
    47221 ccttgattaa aatttcaaag gtttttgggg taacgaccag atcggcattt agttgtttaa
    47281 agccaaaatg tttcgcttga atacaatatt taatgagttg ataaagataa tcttctgaat
    47341 gaacagctcg taatgctcgt aaaataatat cctcctgcgg cagcagatga aaaagcgcat
    47401 gggctttggg tgtatctcct tcatctatcg ctgtgaataa gtctagccaa tagtctggta
    47461 gcctggtttc ccgagttgta ataattggta aatttccata tttttttatc agtgctgaca
    47521 tgtgctttat acgtaaatat tgatcttctt caccgcctgc tggcatgcgg tgcatttgct
    47581 cattgtctat ttcagatggg atttgaatgg cacattcttc agcgtatgga gtggcagaac
    47641 ttttgctttt ccccgttttt gcttttttaa aaaatccttt atccttggcc atatccattc
    47701 ctaaatagct tggttaattt attatatttt agaataggcg agtgagagat attaatccag
    47761 agatttgtta agctcatgac tatcaagaca agaggcgcta ctttatttat aatgcaaata
    47821 gagtttatgg acagaatttc attttgttct gactcgacaa ttcttccgtt atccaattaa
    47881 gtcgagggtc tatggtacaa catgacataa tccaactgtc tccaaaacct tatggtgctc
    47941 atctaatcac taatgaagtg atgcaaaaaa ttacgttgag gggtaccgga ttattacatt
    48001 tatttattca gcatacctct gcggcacttg ccttaaatga aaatgcttcc cctgatgtat
    48061 tgattgatct aaaccatttc tttactcaac ttgttcctga acatcagcat tatcgtcatg
    48121 atatagaggg gccagatgat atgcccgccc atattaaatc aattttgtgt ggctcatctt
    48181 taactattcc atttcaggaa aacaagttgc tattgggtgt ttggcaaggc atttatttaa
    48241 tggaattcag aaaacaagca ggagcaagaa caattgttgc cagtcaattg atttaggatc
    48301 atagggcata actctactca ccatcgaatc aatctgagca acttttgtat ctttctgtgt
    48361 ttcattcata agaaagaaaa acctcctgtt cttaactata ctttatatta ttttgaaacc
    48421 aggacagcct cataatgaat gatcattacg tatctaaaaa aaagatgttt gctgtggttt
    48481 tttgtgtttt tgcgcccaat gaggttttat tagctaatga tactgctgta ggtggggagg
    48541 gatctttacc cattcctgtt tctcagccgc atataaaaat ggtaagtgag ttaatcagga
    48601 tcagcggaaa gaatttaaat gagtctcaga tgaatggttc ctggcattat aattgtaatt
    48661 tcacttttaa aaatacatta aataaagaag tcaccatttc aatggctttt ccctttccta
    48721 taaacgatgg gaattcagaa attgccctgc cagcgggaca gcagactaat gttggtcaag
    48781 cattggttta tgattttctg gttacagtca atgataggca agttccagca cagcgcggta
    48841 atatcgcccc agatcagaat aaaggccttt actatgtgga tgcctatttc tggaaaacca
    48901 cctttccacc cttggcaaca gtcaatattc atcatgatta ttcgacggga gccacttatg
    48961 atgtgatggg gtatcattgg gttcgttatg ttttaaaaac aggtactctt tggcaggata
    49021 gcagtatcgg acatactcga cttgaagtca ttcccaatac accaacgcgt ttatgctctg
    49081 agatagatcc aaaggcagac tacctcaatc ccaccccatc agggatgagc atttcaggct
    49141 cacgagcgga tagaaaatac atctgggatt tacggcattt tcaaccccaa gcagatttgt
    49201 cactttgctt atttacgggg attagttatg ttcgctataa ggtgatttat ccttggttaa
    49261 attcagatga tgcattaagc aaattggcca gattgagtaa taaagaatta cgattcttac
    49321 gaaatactat ttatgcgcaa tatgggcgtc aatttcagtc acccgattta caagaatatt
    49381 ttagcaagaa atggtggtat gtaccaaatc ctgattattc agaccgtatg ctcaatgaag
    49441 aagataagaa attattgtca atgattaatc aggcaaaata aactcaatcg ctgataagct
    49501 ctttataata aatcccatat gaggcctcta aattaataaa agctgattat ggcaggcgac
    49561 tattttacca cccgggtctt tttgttttat gccaattctt gtgtttgttg caggatcgct
    49621 tgagaaaagc attgttgcaa ttttattgag atgcattctt actataatgt cctactaatt
    49681 cagccgtatc tatgatatct cctttcatag cgttcaccac atcctgagca gtggcttctg
    49741 gaccaagatt aaggtaagtg tctaaagcat agagtgtaaa atagtaacgg tgtgtacctg
    49801 caggcgggca gggaccactg taaccggtgg tattccagct gtttaaccca ctggttgcgc
    49861 cagcaggagt tgttgtccct tctgagagct gtttcacttg agcagggata ttaaacaata
    49921 cccaatgtat ccaattacct gtaggagcat caggatcatg cactattaac acataagatt
    49981 gggttcccag attgggatca tgccatatta gtgggggaga ataatttaaa ttgctgcatg
    50041 tgaatttttg aggaattgct gcattcgatg caaatgccgg actttccaat gttaatcctg
    50101 cagaaaaaca cggtgtaaca agtaatgcag agctgactaa tgtcaagaca agatttttaa
    50161 atatggccat cactttccct gtttctattg tttacttacc tgaaattatt gagagtctag
    50221 ttttttatag atttccttaa attatagatt acgattgata cacttgtgat ttgtttttca
    50281 tcaggattta ccaaaagcac caatttattt gttaaaaaaa tgtttaatta ggtcatttat
    50341 tttatcttaa atccctcatt aaaagcattt ttcctcaata ttgttttttt gtaagtccca
    50401 ttcatatgag aaatgccttg tgctgttgtt tataaaattt tctaaaccta taatgaaact
    50461 ccagtatttt taaaggaata atttcaatga aagcaatagg ttactaccac tctttaccca
    50521 ttacagataa taaagcattg atggatctgg atgtttccgt gcccaaagca actggccacg
    50581 atattttagt tgaaatcaaa gcggttgcag tgaatcccgt agataccaaa gtgcgtcgta
    50641 atgttcaacc agaacctcat cagcccaaaa tattaggctg ggatgcttcc ggggtagtta
    50701 ctgaaattgg atccgaagtg actatgttta aaccaggtga tgaagtgtgg tatgccggtg
    50761 atttaaccag accagggtct aattgtgaat ttcatcttgt tgacgaacgc atcgcagcca
    50821 aaaaaccaaa gtcgttaagt tttcaagagg ctgctgcatt acctttaacc agtttaacag
    50881 cttgggagct tttatttgat agattgcaat tatcagcaga agataaaggt attctattaa
    50941 ttactggagc ggctgggggg gttggttcta ttttggtaca actggcaagg caacttaccc
    51001 agttaactat catcggaaca ggagctcgcc ctgaatcaat tcagtggatc ctggataatg
    51061 gggcccacca tgtcattgat cacactaaag acatgaaggc tcaattgcag aatttacatt
    51121 attctgaagt ggattatgtg atcagcttga cccataccga tcaacatgcc aaatcgttag
    51181 ttgaggtcct gaaacctcaa gggaaatttg cattgataga tgaccctcag gatttggata
    51241 ttaagttatt taaactaaag agtatttcta ttcactggga aatgatgtac acccgctcta
    51301 tgtttaaaac agctgatatg attaagcaac atgatatttt aacggaggtt gccagattgg
    51361 tggatgccgg taaaatcaag acaactattg ccgagtgttt gggattgatc aatgctgaca
    51421 atttgagaag agcacatgct ttactggaaa gtggtacttc cagaggcaag atcgttttga
    51481 atggattcaa gtcaatttag ttattctcga aacagcgacg atgcctggaa tgaaacagat
    51541 agagtcactt gatttttaaa tgattctcta agtagcctac gtgcctcagc ttgtctgagg
    51601 catccagaga gcgcttggtt ggtatcgctg acaagcagca gtagccggag gatcactcat
    51661 ttagtttatc taaccttctc attaaaaaca ttatttgcct tgaccgtcga gttttacgca
    51721 aatctatttg tgttaaaaaa atatctttta attaaaaatt ggacatgatg ttttgtcgtg
    51781 ttctatatta attaatacag ggaataaaaa atataagatc aattcaatca caaggaaaaa
    51841 ctatgattat ttatctgcta tggttattag cagtaacatc atttaatttt tcaacttcta
    51901 cgattccagt gtcaaaagcc gagcctcaag ggaatctttt atacagtgaa ataccgtcaa
    51961 tcaagatgcc tttgaatgaa ataaaaacat tgttacaaaa agagggtaat tctcttcaac
    52021 ctgccgttat tgataaagtg ataacgacca tacaatgcgc taatgcttat caagtggata
    52081 gaaataatat
//
LOCUS       DBNCWK010000008        50058 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.008, whole genome shotgun sequence.
ACCESSION   DBNCWK010000008 DBNCWK010000000
VERSION     DBNCWK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 50058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50058
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.008"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(75..1451)
                     /locus_tag="AC06IO_000387"
     CDS             complement(75..1451)
                     /locus_tag="AC06IO_000387"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HIO9873604.1"
                     /translation="MNISVFDLFSIGIGPSSSHTVGPMLAANAFLQLLEQKNLFDKTQ
                     RVKVELYGSLALTGKGHGTDKAILNGLENKAPETVDPASMIPRMHEILDSNLLNLAGK
                     KEIPFHEATDFLFLQKELLPKHSNGMRFSAFDGNANLLIEQVYYSIGGGFITTEEDFD
                     KSTTDTNPPPYPFATATELLKLCKKHHLTIAELMLVNEKTWRSTAEIHKGILDIAKVM
                     DDCINNGCKHDGVLPGGLNLKRRAPDLYRKLIEQKGVKSVFEQSDIMNHLNLYAMAVN
                     EENAAGGRIVTAPTNGAAGIIPAVLKYCQQAHDRMSNEDIYTYFLTAAAIGILYKKGA
                     SISGAEVGCQGEVGVASSMAAAGLTAVLGGTIEQVENAAEIAMEHHLGMTCDPVLGLV
                     QIPCIERNAMGAVKAVNATRMALIGDGQHQISLDKVIKTMKQTGMDMQSIYKETSMGG
                     LAVNLPEC"
     gene            1656..2357
                     /gene="mip"
                     /locus_tag="AC06IO_000388"
     CDS             1656..2357
                     /gene="mip"
                     /locus_tag="AC06IO_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrophage infectivity potentiator Mip"
                     /protein_id="HIO9873605.1"
                     /translation="MKMKLVTAAVMGLAMSTAMAATDATSLATDKDKLSYSIGADLGK
                     NFKNQGIDVNPEAMAKGMQDAMSGAQLALTEQQMKDVLNKFQKDLMAKRTAEFNKKAD
                     ENKVKGEAFLTENKNKPGVVVLPSGLQYKVINAGNGVKPGKSDTVTVEYTGRLIDGTV
                     FDSTEKTGKPATFQVSQVIPGWTEALQLMPAGSTWEIYVPSGLAYGPRSVGGPIGPNE
                     TLIFKIHLISVKKSS"
     gene            2523..3770
                     /locus_tag="AC06IO_000389"
     CDS             2523..3770
                     /locus_tag="AC06IO_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIO9873606.1"
                     /translation="MNKRLFIVFILGFSSGLPMALISSTLQAWYANNGMSVLATGALS
                     LVSLPYAYRIFWGPILDRYSLFHLGKRRSWILTMQILLLLGFNLMAWFIPAQYPKLMA
                     FLALVLACFSATQDVAIDAHRAEYLPPQEHALGASLAVFGYRLALLLSGGLALVMAEK
                     LGWAFTYRFMGFAMAIGMLAIFVSKEPSTEIKEKSNFLVSFIAPVKELLSRPGIIYLL
                     IFIFCYKLGEAFTTTTSGIVMPFLIQGLGFSLDTIGYINKMLGIVSILLGGLTAGFLL
                     MRYSLYRALFFFGLLQALTNVFFVILAMSGKNIVLLAIAVFSDNFVAGMGSTALVALF
                     MRLVNKQFTGTQFSMLVALSTLPRIISGPVAATIQMHIGWVGLYQLSIVFALFFIPFL
                     VLIKEQTKERLQSDTAATPIGAA"
     gene            complement(3887..3973)
                     /locus_tag="AC06IO_000390"
     tRNA            complement(3887..3973)
                     /locus_tag="AC06IO_000390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3937..3939),aa:Leu,seq:caa)
     gene            4156..4542
                     /locus_tag="AC06IO_000391"
     CDS             4156..4542
                     /locus_tag="AC06IO_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3757 domain-containing protein"
                     /protein_id="HIO9873607.1"
                     /translation="MKRYYSTLLVLALAHTSLSAYAHTCPDPQTTSLQWGVPPSPWVE
                     NPFSPNSPQGEENTRFVRANILVAGYGQGVTCTYRNSVGDFSILWQVRTKIPARVDYS
                     WIETMGGYVCTRGLNECQFYVADPSA"
     gene            complement(4646..5989)
                     /gene="nadA"
                     /locus_tag="AC06IO_000392"
     CDS             complement(4646..5989)
                     /gene="nadA"
                     /locus_tag="AC06IO_000392"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIO9873608.1"
                     /translation="MFKNSTTYQPEATLMAIRNDMAICQSDYPLDWYQEDFIPYAQEY
                     QALPDRKLTTVLNWMKPYLAKAQNHFGNKLLLLAHYYMGGEIVRLIEQFGGKIGDSYQ
                     LALMAANNPEKSVIVESAVHFMAESISILANENQHVYITNPKSGCTMEMLAKDFMVEP
                     AFLDLNERYGAENILPVCYMNTSGRVKAMTGAQGGAVCTSSNVKKIFQWAQKQNKKIL
                     FIPDQHMGENVAYWLGIKNLAYWPGGTAGANYSLPAQDKKTLKQFDKAELILFASQCA
                     VHTHYQPEMCDYWKSLGYTTIVHPECRNDVIKVADYSGSTAFIWDLVVHDKAGTKSYA
                     IGTENHMVENLKQYCKNLGIRVVNLAEAPQKDTEKGVGCGCATMSRNDPPHLVALLDL
                     LRQGKSMPYNEVKAGDVVNEFTGTRNRLPVNDQQWVINNAKKALEMMINITEDRV"
     gene            complement(6064..7713)
                     /gene="nadB"
                     /locus_tag="AC06IO_000393"
     CDS             complement(6064..7713)
                     /gene="nadB"
                     /locus_tag="AC06IO_000393"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIO9873609.1"
                     /translation="MSDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPKLNIALI
                     SKAESIECNSRYAQGGIAAAISPEDSLESHISDTVLAGDGLCYLPAVEFIIRQGPDII
                     KQLDQYKVQFKRENDGRFHLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQF
                     FEHHVAVNLITQYHPHRTDNQGEVLGAYILDCQHNRIHTFLAKCVILATGGAGKTYRY
                     TTNPMVATGDGVAMAYRAGARVGNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKN
                     PETGERFMKRYAPDQLELATRDVVARAIFSEIEQGQAGYVHLDISHQSKSFLKKRFPQ
                     IYSTLLSIGIDMSQDLIPVVPAAHYQCGGVLTDVDGRTDLARLYAIGEVAFTGLHGAN
                     RLASNSLLEALVMGSNAARCTLKDIATPLKLIDHIPSWSSPGEVNARRASQINAQWRG
                     LRGEMTSYAGIVRTEAGLEDLLQLILKRKKIIEEYYWKHCITRDFIELRNIILNAELI
                     VRAALSRRESRGGHFREDFPNKSFKAQESIAKLGQADSSLG"
     gene            complement(7710..9080)
                     /gene="purB"
                     /locus_tag="AC06IO_000394"
     CDS             complement(7710..9080)
                     /gene="purB"
                     /locus_tag="AC06IO_000394"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIO9873610.1"
                     /translation="MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWF
                     ESLAANDTIPEVPALDNKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLQD
                     KFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
                     HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHV
                     AAYPEVDWRKHCANFVTSLGLSFNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIW
                     SYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQ
                     RDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTV
                     MRRYNEPNAYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQ
                     LVKAFS"
     gene            9233..10936
                     /locus_tag="AC06IO_000395"
     CDS             9233..10936
                     /locus_tag="AC06IO_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIO9873611.1"
                     /translation="MNTITESYRAGLFQKKYWLQNIISGLIVGVVALPLAMAFAIASG
                     AKPEQGLYTAIVAGLIVSIMGGSRVQIAGPTGAFIVVLSGITAQYGISGLQIATLMAG
                     FILVLFGFARLGSIIKFIPSPVIIGFTSGIAVVIWVGQWQYFFGLPAAGSGHFHEKFW
                     HLLLSFPHMNIATTFLGICALFIVLYSSKLPGLKRIPGPLIALVLVTIIQSVWHFEGV
                     ATIGSLFGGIPQGLPAFTWPEITMGRLIELIGPAFAIAMLGAIESLLSAVVADGMSGT
                     RHNSNRELVGQGIANIVAPLFGGFAATGAIARTATNIRNGGNSPLAGIIHSITLILVL
                     LFLAPLAVDVPLAALAAILFVVSWNMSEARHFIKLIRVAPRADVVILLVTFCLTVFVD
                     LVVAVNVGVIIAVLHFLRRMVNSIQIQRMSEGQLSQELAQEKNYDLPEGVMVYVIEGP
                     FFFGAVETFQSALANTHTDPKVLIIRMRWVPFIDVTGLQAMEEIILNLQKRGVRVLLS
                     EANPLVDAKLRKMGIVQLIGEQNYYKEFSQALDVCRINDSDRKSQPPFEVAKPLASVT
                     Q"
     gene            complement(11139..12842)
                     /locus_tag="AC06IO_000396"
     CDS             complement(11139..12842)
                     /locus_tag="AC06IO_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIO9873612.1"
                     /translation="MSIKNEYETARDHFRLWDKQLKDNILLEKSCLMHTYKTYFPDEP
                     GFIQQLSQFSKAVAEELEPTVMENNLDANLPKIEQYNAIGQRDDRVIHHPAYVRSGDI
                     IYGSNLMQYLLKPGQMKKTLSLFLLSSHAGEAGHNCPIACSAGVIRVLSRYSQLEKTS
                     YYLDKLTHPSFSYNFTGAQFLTEIQGGSDVGANATRASLDKQNQWRISGEKWFCSNAN
                     ADLILMTARFDEETPGTKGLGLFLVPSRLDDGSPNHYYLRRLKQKIGTRTMATAEINF
                     EGALAYPMGDVAEGIHLVMENVLHLSRIFNSFSVLGMSRRAYQIACYYAKNRRAFNHP
                     IIEYPLVKESLAKIKSENLAMLASVFHMARCQDELDALPEPQQTKSQQLLLRTLANIN
                     KYFTAKRSVDNIHHCLDLLAGNGTIENFSSLPRLLRDCIVCENWEGTHFTLWMQTLRD
                     MHKFAIDELFIAHLQQLLEQVQDINPHKALFFEKINELDTTIKTMKLANHEEQSLHIK
                     TIIEHMASLLAALCLAIEIQQGQSPETKQAGLTLFIHDHIASCLLQHDDYMKILNKVI
                     G"
     gene            complement(13071..14069)
                     /locus_tag="AC06IO_000397"
     CDS             complement(13071..14069)
                     /locus_tag="AC06IO_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HIO9873613.1"
                     /translation="MFKRIGLISIALSLNTISLTSEACSALVFNKNKPATVAVNLDWK
                     YREGAFVIHPRNAMMVSSVDYHSMHPLIWKSQYGSVIFHGGNRFKAGPSADGMNEKGL
                     TASILMLDSSSYPSNSELPALKTTEWVQYILDNFQTVQEVIDDSSNYQLVADTYRGIT
                     MNVHLMVSDAEGKSAVFEYLDGKLVIHTQDNLTTPVLTNTDYKSSLALLEEYQDHGGS
                     KALPGGYDSKSRFVRAAHYLKKLPSFIAKEEHIAYAFNGLNVVTQAPGTSSPTQLSMV
                     FDIPTKTIYFRSINEAALRVIPMDSIDFDYLYQPIALNVYQHLSGDVTDKFQPMIG"
     gene            14294..16681
                     /gene="ppsA"
                     /locus_tag="AC06IO_000398"
     CDS             14294..16681
                     /gene="ppsA"
                     /locus_tag="AC06IO_000398"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIO9873614.1"
                     /translation="MTVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPG
                     GFATTADSFREFLSQNGLDKKIYDKLTALDTDDVCQLAVVGKQIREMIIDTPFTPEFE
                     QSVRSSYQKLAQEIGHDNFSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIK
                     QVFASLFNDRAIAYRVHHNFAHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVV
                     FITSSYGLGEMVVQGAVNPDEFYVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLER
                     RVKTVDVDPAERLLFSLSKEEVEQLANQALIIEKHYGRPMDIEWAKDGVNGKLYILQA
                     RPETVKSRDNKQVLERYTLQKRGDVLAEGRSIGQRIGQGKAKVIKDINEMHRVQPGDV
                     LISDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATKTIKDGDV
                     VTVSCAEGDTGFVYSGLLPYEQERLDVETMPELPMKVMLNVGNPERAFAFQSIPNSGV
                     GLARLEFLISNTIGIHPKALLEFDTLKDEELKRYIKEKTIAYDSPVEYYIERLKEGIA
                     TIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEPQEENPMLGFRGASRYVSSNFSECF
                     ALECKAVRRVREEMGLDNVEVMIPFVRTVSEAGNVIEVLKKHGLERGKCGLRVIMMCE
                     LPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGLVASQFDERNDAVKALLHMAI
                     AACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPDSVLQTCLFLAKQSMN"
     gene            16922..18361
                     /locus_tag="AC06IO_000399"
     CDS             16922..18361
                     /locus_tag="AC06IO_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIO9873615.1"
                     /translation="MSNSKWWFLLVCFIPAITFASEGGEHSDPVTSVLLGVTTILFLA
                     IIGRYLARLLNQPGVLGELLIGMIVGNLSYFFGSELVVILREGSAIYDVVKEMLAGIS
                     LNQAVTHVIHNSYYANQVIHALSGAEGTEYLKIAYVVDIFSRYGVIFLLFMVGLETSL
                     SDLKKTGKESILVAVIGIVAPMILGFVVACFIIPNSNYKINLFIGATLSATSIGITAS
                     VLKEMNKLSTREARTILGAATLDDILGLIILAVVSSIVISGAVSIMVVMKIIILALLF
                     FIGTLFMGPFVLRKAVDLFRFLEPWESKLFISFLFIMALAWLASFIQLAAIIGAFAAG
                     VILHDDYFGEQKAHHKDHRSIHHLVSPLETILAPMFFTLIGIQVKLETFYDWNVIVLA
                     GGLSVAAILGKLLSGLGANRRDDRLLIGIGMLPRGEVGLVFASIGRTIGVISDNLFTA
                     IVLMIMITTFIAPPLLKMRYAKPKEDRNA"
     gene            18358..19200
                     /gene="nadC"
                     /locus_tag="AC06IO_000400"
     CDS             18358..19200
                     /gene="nadC"
                     /locus_tag="AC06IO_000400"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIO9873616.1"
                     /translation="MNNVLSQVTFDVKRALQEDVGNGDVTAALLPQQLIVEAEIISRE
                     PMLVCGQPWVNEVFKQVDNTVEMEWRVSEGDFLSTPATLCIIHGTASSILTAERTALN
                     FLQTLSATATQTYHYVQKLKGTKAKLLDTRKTIPGLRLAQKYAVHCGGGLNHRMGLYD
                     AFLIKENHIKACGSVAHAISLARKTDKHLLVEIEVETLEELQEALDAHPDRILLDNFT
                     QDMLEQAVKMNHPKYCELEASGGININNIADIAQTGVDFISVGSITKSIQAIDLSLLI
                     RETL"
     gene            19197..20288
                     /locus_tag="AC06IO_000401"
     CDS             19197..20288
                     /locus_tag="AC06IO_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIO9873617.1"
                     /translation="MSPSIVFTGGGTAGHVTPNIALIKEFRKEGWNVEYIGSVSGIEK
                     EMIEPMDIPFHGVSSGKLRRYFSLKNLLDPFKIVLGIIQSSLLFYKIKPDVVFSKGGF
                     VAFPVVVGAWLNRIPVVAHESDMSPGLANRLSFPFVNKICLTFDAGKKYFKRQDKIEV
                     TGTPIRQQLLTGNRMKGLELCGFNSSKPCLLVVGGSLGAGSINSCIRSALKQLTSEFQ
                     VIHLCGKGKLDSSLVGVEGYCQFEYANEELADLFAASSVVISRAGANSLYEILALGKP
                     HILIPISSQVSRGDQIQNARYFQGLGISVVIQDELLKADVLLQAVQDVMRKKDEIDNK
                     IKALKIESATDKIVAIIKEQAHVQTPRIV"
     gene            20263..21678
                     /locus_tag="AC06IO_000402"
     CDS             20263..21678
                     /locus_tag="AC06IO_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIO9873618.1"
                     /translation="MFKPQGLYDYICQQWQEEILPSLCDYIKIPNKSPHFDAKWEEHG
                     YMEQAVNHIANWCKSHAPKGMTLEIVRLKNRTPLLFMEIPGQIDDTVLLYGHLDKQPE
                     MSGWSDDLHPWKPVLKNGLLYGRGGADDGYSAYASLTAIRALEQQGLPYPRCILIIEA
                     CEESGSYDLPFYIELLKERIGKPSLVICLDSGAGNYEQLWMTTSLRGNLVGKLTVELI
                     NEGVHSGSASGIVADSFRVARQLISRIEDENTGEIKLPQLYCDIPDERIKQAKQCAEI
                     LGEQVYSEFPWIDSAKPVIQDKQQLILNRTWRPALTVTGADGFPAIADAGNVMRPVTS
                     LKLSMRLPPLVDPEAASVAMEKALTQNPPYNAKVDFKIQNGGSKGWNAPLLSDWLAKA
                     ASEASMTYYDKPAAYMGEGGTIPFMSMLGEQFPKAQFMITGVLGPHSNAHGPNEFLHL
                     DMVKKLTSCVSYVLYSFSQKK"
     gene            21770..22087
                     /locus_tag="AC06IO_000403"
     CDS             21770..22087
                     /locus_tag="AC06IO_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIO9873619.1"
                     /translation="MKARLIAILLSFIVIPFSSQAENEPQKTPAKQVLVKGKINLNTA
                     DVHSLTGSFKGIGKKRAEAIIAYRENHQGFKSLEELAEVKGIGQRFVDKNREKLKEIF
                     VIN"
     gene            22283..23320
                     /locus_tag="AC06IO_000404"
     CDS             22283..23320
                     /locus_tag="AC06IO_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIO9873620.1"
                     /translation="MFRKLRGVFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVALRNE
                     SGQKRVAAVGLEAKRMLGRTPGNINAIRPMKDGVIADFFVTEKMLQHFIHKVHENKFL
                     RPSPRVLVCVPCGSTQVERRAIRESAMGAGAREVFLIEEPMAAALGSGMPVEEASGSM
                     VVDIGGGTTEVAIISLSGIVYHQSVRIGGDKFDDAIVSYVRRNYGTLIGETTAERIKH
                     EIGSAFPSRDLFEIEVRGRNLAEGVPRSFTLTSAEILEALQEPLSGIVGAVRAALELA
                     PPELAADIAERGMVLTGGGALLKNIDTLLMEETGLPVLIAEDPLTCVARGGGKALETM
                     DLRGGDFLSTE"
     gene            23298..24206
                     /gene="mreC"
                     /locus_tag="AC06IO_000405"
     CDS             23298..24206
                     /gene="mreC"
                     /locus_tag="AC06IO_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIO9873621.1"
                     /translation="MISSQQNNKHNRLFAKGGHSSLGFVFALAFSILLMFSDYHYRYL
                     DFVRSGFSLIVSPLQYAVDYPVRVIGWIQSLVSAKKALIDENMRLKYKQTMLEAELQK
                     LIVIQRENSQLKELLLTSSKADMRVMAAQILAVDTSQARQVVVLNKGTRDGVYVGQPV
                     LDAKGVMGQVIDVGPMTSTLLLISDSKSAVPVRNNRTGERAILVGTNDIEELSLINLP
                     KTSSIHPGDVLVTSGLGRRYPEGYPVGRVEEVKSIPGEDFVKVTVSPVALLNRNRLVL
                     LIWADSEQEELTAQINERLNAEESTA"
     gene            24203..24682
                     /gene="mreD"
                     /locus_tag="AC06IO_000406"
     CDS             24203..24682
                     /gene="mreD"
                     /locus_tag="AC06IO_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIO9873622.1"
                     /translation="MSVLHIRLGLGFLVALALSILPMPELISIFRPPWVLLLVLYIEY
                     YLPGKFKLTALLIAGLLLDVLLSTVIGEHSFALLLVTWIASTKSRRVQFFSLIQQVCL
                     IGFFCFLYQSIIIFIDALLGFNYNLIMPVASAILGMIIWPWIRVLGYSSLLTRSVYR"
     gene            complement(24618..25220)
                     /locus_tag="AC06IO_000407"
     CDS             complement(24618..25220)
                     /locus_tag="AC06IO_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIO9873623.1"
                     /translation="MPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQ
                     SEGLLVLTDDGKLQHRLTHPKFEKKKHYWVQVEGAPTDKDLQPLRKGLVIKDITFLPA
                     EAKLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQIRKMTAAIGFPTLRLIRHQ
                     IGDWRLDQLQPGEYKLINLTNDKLNELTRKSNPRLGSRAK"
     gene            25481..26179
                     /locus_tag="AC06IO_000408"
     CDS             25481..26179
                     /locus_tag="AC06IO_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HIO9873624.1"
                     /translation="MKWVLVVMAIIAQTSCAVNHRVMVGDEPVIIQQTKGQGKTFVHV
                     HHNEQTALKAAKAVIRKEGGSLITLIHSGGRNIVFHLHNQRYEFDPNRIFTDKGIKKT
                     LTQYSYYTPEAHQEVKKLADKIKALLPEGKVIAVHNNSSYSLKDYLPGHELAKDAKAL
                     YMNPDNYFRNFYLVTKLSDYLRLKMQGFNGVLQKPSATDDGSLSVYLAKRDYINVEAG
                     YDQLAEQIKMLLHA"
     gene            26281..27549
                     /gene="icd"
                     /locus_tag="AC06IO_000409"
     CDS             26281..27549
                     /gene="icd"
                     /locus_tag="AC06IO_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIO9873625.1"
                     /translation="MESSMTYDKIKVPAQGEAITVAADHSLHVPDNPIIPFIEGDGIG
                     VDVTPPMIRVVDAAVQKAYGNKRKISWMEVYAGEKATKVYGSDQWLPKETLDAMKKYV
                     VSIKGPLTTPVGGGIRSLNVAIRQDMDLYVCLRPIRYFNGVPSPVREPWKTDMVIFRE
                     NSEDIYAGIEWQADTPEAKKVIQFLTKEMGVKKIRFPEHCGIGVKPVSREGTTRLVKA
                     AIQYAIDNDRSTVTLVHKGNIMKFTEGAFKDWGYQVARDSFGAKEYQGGPWMEFKNPK
                     TGKQIIINDVIADAFLQQILLRPEDYSVIATLNLNGDYISDALAAQVGGIGIAPGANI
                     SDQMAVFEATHGTAPKYAGQNKVNPGSIILSAEMMLRHMGWYEAADLIIRGMEGAIEA
                     KTVTYDFERGMQGATLVSSSGFADAMIKHM"
     gene            27951..28286
                     /gene="clpS"
                     /locus_tag="AC06IO_000410"
     CDS             27951..28286
                     /gene="clpS"
                     /locus_tag="AC06IO_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIO9873626.1"
                     /translation="MSRQNLEEIIQTGIADTELSTEISTAIKRPRKYKVLLLNDDYTP
                     MDFVVEVLKHFFHLNEEVAIQVMLQVHFQGKGVCGVFTRDIAETKVALVNEYARMNQH
                     PLLSSMEPE"
     gene            28318..30585
                     /gene="clpA"
                     /locus_tag="AC06IO_000411"
     CDS             28318..30585
                     /gene="clpA"
                     /locus_tag="AC06IO_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIO9873627.1"
                     /translation="MLNKELEFTLNLAFKEAKEKRHEFMTVEHLLLSLLDNPAAGNVL
                     QACDANIEALRRDLIEFIDETTPRIPEDELDRETQPTLGFQRVLQRAVFHVQSAGKTE
                     VTGANVLAAIFSEQESQAVYFLRRENITRLDVINYISHGVSKYQNNDMHENMNSSMEE
                     DMMTPEGNESPLDSYCTNLNRRAKQGKIDPLIGRHEEIQRTIQVLCRRRKNNPLLVGE
                     AGVGKTAIAEGLARRIVDGEIPDSIRGCVVYSLDLGALLAGTKYRGDFEKRLKAVLKQ
                     LGQQEGAILFIDEIHTIIGAGAASGGVMDASNLVKPLLANGELKCIGSTTYQEYRGIF
                     EKDRALARRFQKIDISEPTIDETFEILKGLKGKLEAHHGVKFSIASLKAAAELSAKYI
                     NDRFLPDKAIDVVDEAGAYQNLLTANKRKKIISVTEIESVVAKIARIPIKKVSARDKD
                     TLRNLERDLKLLVYGQDQAITALASAIKLGRSGLREPQKPVGCFLFAGPTGVGKTEVT
                     RQLANVLGIELLRFDMSEYMEKHTVSRLIGAPPGYVGYDQGGLLTEAVTKNPHAVLLL
                     DEIEKAHPDVFNLLLQIMDHGTLTDTNGRQADFRHIILVMTSNAGASEIVRNSIGFST
                     QDNTNDGLEVIKKQFSPEFRNRLDAIINFAPLDTQTIGLVVDKFIMELDEQLSHKGVT
                     FTVDKSAREWLMEHGYDKAMGARPMARLIQENIKKPLADELLFGKLMHGGHVTLRVKD
                     GKLHFDSHDHREGVC"
     gene            30809..31588
                     /locus_tag="AC06IO_000412"
     CDS             30809..31588
                     /locus_tag="AC06IO_000412"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9873628.1"
                     /translation="MLSVIIITKNEEANIRRCLESVHFADEIIVLDSGSTDNTLAIAR
                     EYTDKVFSTDWQGYGIQKDRALQKAQGDWVLNLDADESVSSELRQEMLQAISSDTADA
                     FRIPIQMIFYNQVLKYSGSPKRHIRLFKRENACYSKDIVHEKVLIPVNARVGKLKKPI
                     WHHSFSDVHHVLYKLNKYSSYSAKIRIESNEKVGLVKTFFSALWMFVRCLFLQKGFLD
                     GRAGFLFAVFGAQGAFYRGVKQIYKDRDINKLPSVNHIKEE"
     gene            31591..32841
                     /locus_tag="AC06IO_000413"
     CDS             31591..32841
                     /locus_tag="AC06IO_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIO9873629.1"
                     /translation="MRLTIMDTKGILLAPFFFISFMFFIPISPTIKSICFICSLIAVL
                     ITPQYRKHIVYAYNTLWGRAAFCLFLFVVIASLWSPAPYSMQWMVIGKYCKLIYLPIL
                     ATGFINPKVRNWSINSYLAAIFITCVISILKAKNIIASEDPGEVFYNHIITGFMVALA
                     AYLTGLLAFQNSGWLRLIYIFMMFLTSYQVLFINTGRTGYLVYFILMLLLLIQKLNFK
                     QALAGIVLFSGIIALAYHHSPVMQTRIYDLIQDIKLLKQHNKNTSIGYRMQFHHYAKQ
                     LMLKHPFIGIGTGGFKYSYSQDVPVPSWGKELTDPHSQYWMTLAEQGLIGLVLLVIFL
                     ASLFITSFQLTSSRPILLGVLFSFCIGSISDTILCYSTAGYLLVVMSALCFGELIEKR
                     RVKGVEEDHLVPLSAHAPAAISQT"
     gene            complement(32842..33177)
                     /locus_tag="AC06IO_000414"
     CDS             complement(32842..33177)
                     /locus_tag="AC06IO_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurogenic locus notch"
                     /protein_id="HIO9873630.1"
                     /translation="MNILKPKAFLLASVFALSASPAFAADGCCSKMGGINYCDSSAGR
                     LVCNNGFYSTCYCTRHAVMDLQFLMGCCLWHGGVYPQLNSSGLVVCNDGYVSEECSLQ
                     KPVEQISVY"
     gene            complement(33347..33943)
                     /locus_tag="AC06IO_000415"
     CDS             complement(33347..33943)
                     /locus_tag="AC06IO_000415"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIO9873631.1"
                     /translation="MINELTASLNMIVTQTQNNLKILGVILLIPWSIFFISYFISNKI
                     LLLGIIPRHILGLPGILLAPLLHANFNHLFFNSIPLLVLSNFILINGVNYYLIVTVMI
                     TLFSGTLIWCFAKRGIHIGASGLITGYWGFLVYNIYQTGTLTTIILGLLSLYYFAGIF
                     LGIFPGEKGVSWQGHLFGLLAGFLTSYLLEVYPQILIY"
     gene            complement(33940..34842)
                     /locus_tag="AC06IO_000416"
     CDS             complement(33940..34842)
                     /locus_tag="AC06IO_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIO9873632.1"
                     /translation="MKKLLHSVACLLILISSAKLSYGLALPENHSVNGGLTIIPIDFN
                     QKPEAYFEGKRIPVLPSTQPRQWLLIVAIPLKNTNSVQYINVTKPIKASIPFHVSEKF
                     YSTQFLNIKDISKVDPQPEDLIRIEKETKKLTQIFANYSNTNPFEKQFTAPLRGPITS
                     LFGLKRVYNKQPRDPHSGLDIAANEGKPVYAVNQGVVADTGDYFFTGNTVILDHGMGV
                     FSVYAHLSKILVKTGETVKQGQELGLVGMTGRVTGPHLHWTMVVNQTLVEPLLFVPFR
                     KITVTPVIPDKKSNKSTQDATVKS"
     gene            complement(34851..36182)
                     /gene="xseA"
                     /locus_tag="AC06IO_000417"
     CDS             complement(34851..36182)
                     /gene="xseA"
                     /locus_tag="AC06IO_000417"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIO9873633.1"
                     /translation="MSSQLPILTVSQLNRQVKGFLENEIGLVHVEGEISNLSKPSSGH
                     YYFTLKDSTAQIRCAFFKNRHSNSLLRNFNDGQQIVATGKLSLYEARGEYQLIVEEIV
                     EAGMGILYQRFEELKIKLASEGLFNPERKKTLPRIPETIGIITSPTGAAIQDILSTLD
                     RRFPIARIIIYPSEVQGQTAPQQLVNSLKLANAHKRCQVLILARGGGSIEDLWAFNDE
                     YLARQIAISEIPVVSGIGHETDFTIADFVADYRAETPTAAATAVTPNCIELFNILDTA
                     IYRLHDAIIRLIKGLQLKLNHLIDKIASPRQAISTYWQTLDYLERQLISAMTQFINLN
                     INKVNIFSTQLQASNPKIQIERTKIQLQQLIMQLTQEIRIKVNQLKNQLSTNLSTLHA
                     VSPLATLDRGYAIVSKNQRILFAAQQAQIGDTINIRLAKGSLACEVTQIKD"
     gene            36332..38056
                     /locus_tag="AC06IO_000418"
     CDS             36332..38056
                     /locus_tag="AC06IO_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIO9873634.1"
                     /translation="MRKALFVVAMLTSSYTTADTLYEAVQYGMIANPDILLNTAKGLS
                     AKQAIDKAKGGLYPSIDVTGGFGRQRSVNPTSAAIDDTPSTTLNIVESAVELRQRLFA
                     GGGIINEVKRNQYLTEAQKWKTQGIAEDLALEITKNYFAVLLHERLYAYSIENLKAHK
                     AVFKMIKERADAGITRTAEVDQANARLALAEANKISALADLQEVKINYAKTVGKWPEN
                     LQMPHVPARKSLPNNLARIIEKGLDNHPTVKSSYADIKEAKAQYDVARAAYYPEVNLV
                     LNSSKNKNLGGLIGPNDSDTVAVRMNYNAFRGGADAARIRETAYQVQEAYETKNRTLL
                     ELKETIRLAWNAYVASALRIQPLKQHVTASRKTRTAYQDEFKVGKRTLLDLLDSQNEY
                     YQSQIELARAENDEILSRYRILNGMGCLLKYLNLRLPVNVVNNDVFSSAQTNILLNRK
                     MDEIPYPNNTDNPMVLTHPVKNMEKTRLTKSMVDKNTTYPQQITPKLWYISAGSFRDR
                     NNAIALVKRLNGLGFTAFMSPYKDLLSVFVGPYEYRGHAGNGMERLKELAHVEGVLVT
                     FKEPPKKA"
     gene            38007..38741
                     /locus_tag="AC06IO_000419"
     CDS             38007..38741
                     /locus_tag="AC06IO_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HIO9873635.1"
                     /translation="MLKESWLLSKSRPKRLSVIAKRFQNVLLESLRYRLVLSIWTGLI
                     LCFITSFSTQAAPLISVEKIQKLAQSYQGDTRKRFTAWANLIESLKKKPVKIQLEKVN
                     SFFNQFNYETDPITGASDDYWKSPVEFIVDGGGDCEDFAIIKYFTLVAVGVPSDQLRI
                     TYAASLTLNQAHMVLSFYPTPESEPLILDSLESKILKASARPDLKPVYSFNAEGLWLA
                     KTRGESSLMGDSKSLGKWDALMKRME"
     gene            38757..40676
                     /locus_tag="AC06IO_000420"
     CDS             38757..40676
                     /locus_tag="AC06IO_000420"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIO9873636.1"
                     /translation="MTLTKKMAVGVLIMLLFIFIGTYFITMNNARNFFIQQLESNAQD
                     TATSLGLSLSQSLINHDVPTMDSMVKAVFDRGYFSSIKVKDIKGKVIILKKQLPQESD
                     IPQWFVNLIKWPSTEKSSLIMDGWMQAGVVLVASDPSYVYASLWRNAVEMVNAYLIFA
                     LVALVLSYCFIKYLLQPLKRVTAQALAISEHEFPVETKIPKTPELRQVTLAMNQMVTK
                     VKSLFQDQLKQTESLRTQVYQDSLTGMSNRRYFLQHLALILDNEDEFIPGYVMMLVID
                     GLDELNQKQGYQQGDQLVLTVAKICKSYWKQSSVSTLARINGTTFALISHERDPLVFE
                     KECKEFEQILSQGITDIKICETHMGAASYFLHQPVSNLLSIVDQAVKKARETGVFYCQ
                     KEHDTYKYPQLISGDEIRKSLEQKKISLYAQAVTDGKNCFHKEVFVRIRNQEGEELGA
                     GYFIPVAEKLGLAYLIDQYVLNELTVMDIATHTHFALNISEDTLTNKVNSTGYLRQLE
                     ATPAAVLRNLSLEINEAHVLSHFSNSKFFIKQAKKLGVTVGIDRVGIKFSPLHYLSDL
                     NIDYLKLHGSLVTDIDENESKQFFIHYFNEMAKTMDIAVVATQVESEAQWQALQIVHI
                     PWGQGRFLSSVELIK"
     gene            40812..41537
                     /locus_tag="AC06IO_000421"
     CDS             40812..41537
                     /locus_tag="AC06IO_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HIO9873637.1"
                     /translation="MNYFGNLQIRFSKTFLLLLIVSSLTACVDLTGARNTYIKKEVQP
                     AISDVPNKKSKPQNMSSKKGEVHTMLGGLGIFSKGMYTLRDKVSEKYHITAHSTMWYN
                     AGAVSRYIIDHYYQEKDHKPIILVGHSLGANEQIKVARNLNAAGVPVALLVTVDAVSQ
                     TIVPPNVKQALNVYKPGFVPMFSGLKLRAVDPQLTKIDNVNVDSIKGIKVNHFTIDKD
                     EILQAMIMDKVDKVLVDGNKKGA"
     gene            41515..42825
                     /locus_tag="AC06IO_000422"
     CDS             41515..42825
                     /locus_tag="AC06IO_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIO9873638.1"
                     /translation="MEIKRVLKDRLSARVCVIGAGPSGIAAIKNLQEHGITNVTVFEK
                     NNQIGGNWVYDEQNEHSSVYETTHIISSKRWSEFEDFPMPVDYPDYPSHAQLLKYFQS
                     YVEHFHLARYIRFNTVVQKVHRLDDNTWHVIYEDAQGIHEACYDYLLVANGHHWDPFM
                     PVYPGVFDGQILHSHQYKKASIFKGKRVLVVGGGNSACDVAVEISRVAPGTCISMRRG
                     YHIFPKFIFGKPTDVAVAKIQWMPSWLRQKFISLVIRGLQGRYAKYKLMKPDCGPLEI
                     HPTINSELLYFIRHGKIHPRPGISRFEGNTVHFTDGTQGEFDTIIFATGYQISFPFFD
                     KSCIDFSNSTTIPLYRKMMHPEFENLYFIGLCQPQGCIWPLADYQSKIVARIIAGSLK
                     RPDQLHRKIAKEINKPHHRFKSHMRHALEVDYHIFRKELLEMLG"
     gene            complement(42948..43796)
                     /locus_tag="AC06IO_000423"
     CDS             complement(42948..43796)
                     /locus_tag="AC06IO_000423"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9873639.1"
                     /translation="MNVVIITGAASGIGHALSQVCLSNGKTIVMVDKNQDKLHTEAQS
                     FMTKFSPENVICYACDVTKPSEVERLAEYSCKQLGQVDWIFNNAGIIGNLLPAWELQE
                     NDINQVMQVNLHGMLNVIRSFMPYLFKQNFRSHIINMASLYALCTGSQMAAYSMSKHA
                     VLALSESLYFDLNRLKKPVDVSVVFPSFTDTSLLASSGKLNHSPIHNQLNNLLAHSRP
                     AIEVAEHIVREVEQKRFYILPDKEVKGYCEDRTKAILLQENPHRNSVEQLMCSLLKRQ
                     SSEANL"
     gene            44099..44287
                     /locus_tag="AC06IO_000424"
     CDS             44099..44287
                     /locus_tag="AC06IO_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873640.1"
                     /translation="MNTTEIPEQPIEYPEQPIEYPEEPSEYPEQPLEYPDNPEPLEPG
                     YPELPEPEPSQTPEPGFE"
     gene            complement(44293..45069)
                     /gene="trpC"
                     /locus_tag="AC06IO_000425"
     CDS             complement(44293..45069)
                     /gene="trpC"
                     /locus_tag="AC06IO_000425"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIO9873641.1"
                     /translation="MNSILERIAKHKLEEVAVAKKNKPLHVLSKQQPGEIRDFITALK
                     SNASPAVIAEIKKASPSKGLIRKDFNVAEIAKIYTQNGARCLSVLTDIEFFQGHPDYL
                     ALAKSKTTLPVLRKDFIIDSYQIYESLVLGADCILLIVALLDDVQLMDFCQLAQELKM
                     SVLVESHTQDELERALRLPTPLIGINNRSLHNFKTDIQLSIQLKQFVPKDKIIITESG
                     INTREDIKLMQSHGINAFLIGESLMRADNIGKALQKLMTD"
     gene            complement(45062..46096)
                     /gene="trpD"
                     /locus_tag="AC06IO_000426"
     CDS             complement(45062..46096)
                     /gene="trpD"
                     /locus_tag="AC06IO_000426"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIO9873642.1"
                     /translation="MKPKLLFEQLLSRQDLSSDQMQEVIHACMTGEFSDVQIATFLAL
                     MRMKGETVNELTAAAKVMRQLAHKIDLGNPLIDIVGTGGDGRNTFNVSTACSFVVAAA
                     GIKVAKHGNRSVSSRSGSADLLEQAGFILNLSDSQVQNCINQCQLAFLFAPHYHPAMQ
                     HARAARQQLGIRTLFNLLGPLINPAQVKRQVVGVFSTNWLKTIATVLANLGSERSLVI
                     SSQDGLDEISIAAKSEVVEYRDGNFKQWFISPEDYGLKHSSLDAIIVDSPEQSLHLIQ
                     SVLSGDSGPARDIVLLNSAAAIYCAKDGISFDAAIEEAKIAIDSGKANHCFNKLRLLT
                     QTLNKESNHE"
     gene            complement(46074..46652)
                     /locus_tag="AC06IO_000427"
     CDS             complement(46074..46652)
                     /locus_tag="AC06IO_000427"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIO9873643.1"
                     /translation="MLLIIDNYDSFTYNLVQYFQSLNQKVAVFKNDEIQIDAIRQSAP
                     QYLVISPGPNSPDESGISIEVIKEFHKHIPILGICLGHQCIAQAFGGKIIPAPEIMHG
                     KTSEITHHYQGLFTGIPNHFQATRYHSLAVDIMSLPNCFSIDAWADDTIMAISHRQFP
                     LFGLQFHPEAILSQFGLQILENFLRYETQTSI"
     gene            complement(46686..47411)
                     /gene="lptB"
                     /locus_tag="AC06IO_000428"
     CDS             complement(46686..47411)
                     /gene="lptB"
                     /locus_tag="AC06IO_000428"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIO9873644.1"
                     /translation="MSLLEAKQLKKSFKGRTVVNGVSIHIKQGECVGLLGPNGAGKTT
                     CFYMIVGLQSCDEGHIILNGTDITQDAMHLRARHGISYLPQEASVFRKLTVAENIMAI
                     LELRKDLDDQKREEKLDLLLQEFHISHIENSLGMALSGGERRRVEIARALAIEPSFIL
                     LDEPFAGVDPISVIDIKKIILHLCDKGIGVLITDHNVRETLDICERAYIVSQGKILCE
                     GTPKAILANQQVRQVYLGEEFAL"
     gene            complement(47408..47917)
                     /gene="lptA"
                     /locus_tag="AC06IO_000429"
     CDS             complement(47408..47917)
                     /gene="lptA"
                     /locus_tag="AC06IO_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIO9873645.1"
                     /translation="MTQLKVKYIILLLLMLCSISGNALPFDKEKMVQVVSDSADLNQK
                     NHQGVYIGNVEFVQGSTNLRAEKAITQGDAKNQLTLAIAEGSKDKQAHYWTTTAPDKP
                     PFHAYADTIKYYPLKHLIELIGNARIEQGPNSLSAAKISYDTQEQHVLSHSDEKTRTT
                     IIIYPEKKS"
     gene            complement(47898..48467)
                     /gene="lptC"
                     /locus_tag="AC06IO_000430"
     CDS             complement(47898..48467)
                     /gene="lptC"
                     /locus_tag="AC06IO_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIO9873646.1"
                     /translation="MNITKQAIWLFLTLLILAGSGWYYAHFNQAIINKKTLSNEVDTT
                     ISQLTVKQFNSEGVLINLLTTPQVEYIPNNNLHLLKNPHIIIKQDNQPAWEINSLRAK
                     SFDGGKQITFIEQVVVHQNPGENTRESTLKTEEITYYPKEKKATTSLFVTFEQPGNII
                     QSTGMNAYLDEKRVELLHKARGSYDPAKG"
     gene            complement(48464..48991)
                     /gene="kdsC"
                     /locus_tag="AC06IO_000431"
     CDS             complement(48464..48991)
                     /gene="kdsC"
                     /locus_tag="AC06IO_000431"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HIO9873647.1"
                     /translation="MNELLEKAKKIKCLICDVDGVLSDGLLHIDNHGNELKSFHVQDG
                     MGLKLLMAAGIQVAIITTAQNAVVDHRMEQLGITHYYKGQVDKRSAYQHLKKTLGLND
                     EEFAYIGDDLPDLPLIQQVGLGVAVSNAVPQVLEFADWRTERTGGRGAVRELCDLILN
                     AQNKAELAITGYLKQ"
     gene            complement(49005..49967)
                     /locus_tag="AC06IO_000432"
     CDS             complement(49005..49967)
                     /locus_tag="AC06IO_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIO9873648.1"
                     /translation="MNFCTLGLAVIETEAQAVFELTQRIDEHFEKACELLLSCKGRIV
                     VTGMGKSGHIANKLASTFSSTGSPAFFMHPGEASHGDLGMITRQDIVVAISNSGNTHE
                     IVTLLPLLKRLEVPLITLTGNKQSTLAKSADVNLDVSIKQEACPLGLAPTTSTTVSLV
                     MGDALAISLLQARGFSAEDFALSHPGGALGKKLLLKIDDLCHTGEQLPLANENATVSD
                     ALIEVTNKKLGMTCVVDNHGYLVGVYTDGDIRRTLTRQFNINTTLIKDVMTKNCRTIS
                     KGMLAAEALAIMQKYSITSLVVVENDNRPYAVLHLHDLLKAGVF"
BASE COUNT        14597 a         9045 c        10621 g        15795 t
ORIGIN      
        1 cacggtcgcg gctcaggttt aattatcatt tgatttaatc atccgagccg cgaccgtgag
       61 ggagcgggag ataactaaca ctcaggcaag ttaacagcca acccacccat ggaagtttcc
      121 ttatagatac tttgcatatc catgccggtt tgcttcattg ttttgatgac tttgtccaaa
      181 gagatttgat gctgaccatc acctatgagt gccattcggg ttgcattaac tgctttcaca
      241 gcccccatgg catttctctc gatgcatggg atttgcacaa ggcccagaac gggatcacag
      301 gtcattccca gatgatgttc cattgcaatt tctgcagcat tttcaacctg ctcgatggtt
      361 ccgccaagaa ccgctgtcaa accagcagct gccatggaag aggcaacacc aacctcacct
      421 tggcatccta cttcagcgcc agaaattgaa gcgccttttt tataaaggat tccaatcgct
      481 gcggcagtca gaaaataagt gtagatgtct tcgttgctca tcctgtcgtg agcttgctga
      541 caatatttaa gaacagctgg aataataccg gcagctccat ttgttggggc tgtgacaatt
      601 cttcctcccg ctgcgttttc ttcattgaca gccatagcat aaaggttcaa atgattcata
      661 atatctgact gttcaaaaac acttttaacc cctttttgtt ctattagttt tctatacaag
      721 tcaggtgccc gtcgctttaa attaaggcca ccggggagca ctccatcatg tttgcaaccg
      781 ttgttgatgc agtcatccat tactttggca atatcaagaa ttcctttatg aatttctgct
      841 gtacttctcc atgttttttc attgactaac atcaattcag cgatggtcaa gtgatgtttt
      901 ttgcataatt tcaataactc agttgctgtt gcaaaggggt aagggggggg attggtatcg
      961 gttgttgatt tatcaaagtc ttcttcagtt gtaataaaac caccgccaat agagtaatag
     1021 acttgctcaa tcagtagatt agcgtttcca tcaaaagcag aaaagcgcat tccattactg
     1081 tgtttgggta ataattcttt ttgtaaaaat aaaaaatcgg tcgcttcatg aaaaggaatt
     1141 tctttcttgc cggccaaatt gagtagatta gaatcaagga tttcatgcat tcttgggatc
     1201 atggaggcag gatcaactgt ctcgggcgct ttgttttcaa gaccatttaa aatcgcttta
     1261 tcagtaccgt gccccttgcc agtaagcgcc agcgaaccat aaagctctac tttgactctc
     1321 tgagttttat caaacagatt tttttgttcc agtaattgca aaaaagcatt ggcagctaac
     1381 atagggccta ctgtgtgtga gcttgatggc ccaattccta tagaaaataa atcaaaaaca
     1441 ctaatgttca tcgccgttaa aatctcttgt tcattattag gggcaagtgt agaaggatat
     1501 tacctttttg tccattatat aattaaatga tagcttatga ctggtaattt tatgtaaatt
     1561 taggcagaat tactgggcga tttgtttttg tttaattttg ctcaatttat tgtgcagtat
     1621 gagaacttaa gtgtaagact aaaaggggat tgtttatgaa gatgaaattg gtgactgcgg
     1681 ctgttatggg gcttgcaatg tcaacagcaa tggctgcaac cgatgccaca tcattagcta
     1741 cagacaagga taagttgtct tatagcattg gtgccgattt ggggaagaat tttaaaaatc
     1801 aaggcataga tgttaatccg gaagcaatgg ctaaaggcat gcaagacgct atgagtggcg
     1861 ctcaattggc tttaaccgaa cagcaaatga aagacgttct taacaagttt cagaaagatt
     1921 tgatggcaaa gcgtactgct gaattcaata agaaagcgga tgaaaataaa gtaaaagggg
     1981 aagccttttt aactgaaaac aaaaacaagc caggcgttgt tgtattgcca agtggtttgc
     2041 aatacaaagt aatcaatgct ggaaatggtg ttaaacctgg taaatcggat acagtcactg
     2101 tcgaatacac tggtcgtctg attgatggta ccgtttttga cagtaccgaa aaaactggta
     2161 agccagcaac ttttcaggtt tcacaagtta tcccaggatg gacagaagct ttgcaattga
     2221 tgccagctgg atcaacttgg gaaatttatg ttccctcagg tcttgcatat ggcccacgta
     2281 gcgttggcgg acctattggc ccaaatgaaa ctttaatatt taaaattcac ttaatttcag
     2341 tgaaaaaatc atcttaagtt tttttgaatt aaagtcatac aaaacgcatc cctctcattt
     2401 agagagggat gctctctttg taaaggctaa tgatcttcat aaaaggtgtc agcctaacac
     2461 cactgaggaa attaaaatat gtctgtttta aaggctatga ctcatactga gtgaacaaag
     2521 gaatgaacaa gcgcctgttt atcgttttca ttttaggttt ttcttccggt ttacctatgg
     2581 cattaatcag cagtaccttg caagcatggt acgctaataa tggcatgtca gtgcttgcga
     2641 cgggagcttt aagtttagtc agtctacctt atgcgtatcg tattttctgg ggtccaattc
     2701 ttgatcgcta ctctttattt catttaggga aaaggcgaag ctggattctc accatgcaga
     2761 tactactgct tctgggtttt aatctgatgg cctggtttat tccagctcaa tatcctaaat
     2821 tgatggcatt tttagcttta gtattagcct gtttttcagc cacacaagat gtggcaattg
     2881 atgctcatcg agcagaatat cttcccccac aggaacatgc attgggcgcg tctctggctg
     2941 tgtttgggta tcgattggct cttttgttat ctggcggttt ggctttggta atggcagaaa
     3001 aattaggctg ggcttttact tatcgcttta tgggatttgc aatggcgata ggtatgttgg
     3061 caatatttgt cagcaaggaa ccctctacag aaataaaaga aaaaagtaat tttcttgttt
     3121 cgtttattgc accagtgaaa gaacttttaa gccgcccagg gattatttat cttttaatat
     3181 ttatattttg ttataaatta ggtgaagcat ttactacgac gactagcggt atagtaatgc
     3241 cttttttaat ccaaggcttg ggtttctctc tggatactat cggctatatc aataaaatgc
     3301 ttggaatagt ctctatcttg ttaggcggtt taacggcagg ttttttatta atgcgatatt
     3361 ccttgtatcg cgcattattt ttctttggct tactacaagc actaactaat gtcttttttg
     3421 tcatattagc gatgtcagga aaaaatattg tgctgttagc aatcgcagtg ttttcggata
     3481 atttcgtcgc agggatgggt tctactgcat tggttgcttt atttatgcgt ttggtgaata
     3541 aacagtttac tggaacacaa ttttctatgt tagtcgcatt atcgacttta cccaggatca
     3601 tttcaggtcc tgtagctgct accattcaaa tgcatatcgg ctgggtcggt ttatatcagt
     3661 tatccatcgt tttcgctttg ttttttatac cttttctagt gttgatcaag gagcaaacaa
     3721 aagagaggtt acaaagtgat acagctgcta caccaattgg tgcagcttaa tttctatttt
     3781 aatcagatca aaataggttg ttttatacag ctctggacta actgaggatt aaataatttt
     3841 agtttttggt atctgttgta ttgaaattat ttgatagttt attgattggt accgagggtg
     3901 ggactcgaac ccacacggtg tcaccaccac cggattttga atccggcgcg tctaccaatt
     3961 ccgccacccc ggcacgcgcg gtcattatag caaagaaata aattctaaca atggtttagt
     4021 tcatttatta tttgaaaaat tacatggttg attgcatttt gattattaat ttgacaatta
     4081 cttgagcttt attaaataat gagatcatat caagaataaa ttggaattat caggattaga
     4141 attgatggag tctttatgaa aagatattat tctacactat tagttttggc tttagctcat
     4201 accagtttga gcgcttacgc gcatacatgt cctgatcctc aaaccacgtc attacaatgg
     4261 ggtgtgcctc catcaccgtg ggtagaaaat cctttttccc caaatagccc tcagggagaa
     4321 gaaaatactc gcttcgtgcg agctaatatt ttagtcgcag gttatggtca gggtgtgact
     4381 tgtacttata gaaactccgt aggtgatttt tctattttat ggcaggtgcg gaccaaaata
     4441 cccgctcgtg ttgattatag ctggatagaa actatgggag ggtatgtctg cacacgtggt
     4501 ttgaatgaat gtcagtttta tgtggctgat ccttcggctt aatatacccg cttccttacg
     4561 gtcgcggctc ggatttgata agcactgttg atgcaaattc gagccgcgac cgtaaggaag
     4621 cggacaatac actagctaac acgtattaaa ccctgtcttc agtgatatta atcatcattt
     4681 ccagggcttt tttggcatta ttaataaccc attgttgatc gttgactggg agtctgtttc
     4741 tggttcctgt gaattcgttg accacatcac ctgcttttac ttcattgtaa ggcatcgatt
     4801 tgccctggcg aagtaaatcg aggagagcga ccagatgtgg cggatcattg cgagacatgg
     4861 tagcacaacc acatccaact cctttttcag tgtctttttg aggggcttca gcaagattta
     4921 caacacgaat tccaagattt ttacaatatt gctttaagtt ttctaccatg tggttttcag
     4981 ttccgatggc ataacttttt gtacctgctt tgtcatggac taccagatcc catatgaatg
     5041 cagtagaacc tgagtaatca gccactttga tgacatcatt acggcactct ggatgtacaa
     5101 tggtagtgta tcccaggctt ttccaataat cacacatttc aggttgataa tgggtatgaa
     5161 ccgcgcattg acttgcgaat aaaatcaact ctgctttatc aaattgcttg agtgtttttt
     5221 tgtcttgcgc tggtagtgaa taattagcgc cggcagttcc tccaggccaa taagcgagat
     5281 ttttaattcc taaccagtaa gcgacatttt ctcccatgtg ttgatcagga ataaacagga
     5341 ttttcttgtt ttgtttttga gcccattgaa aaattttttt gacattggaa cttgtacaaa
     5401 ccgcacctcc ctgggcaccg gtcatggctt tcacacgacc agaggtattc atgtagcaca
     5461 caggtaaaat attttcagca ccataacgct catttaaatc aaggaaagca ggctcgacca
     5521 taaagtcttt ggcaagcatt tccatggtgc agcctgactt gggattggtg atgtagacgt
     5581 gttgattttc attagccaaa atgcttatgg attccgccat aaaatgaact gctgattcca
     5641 ctatgactga tttttcaggg ttattcgcag ccatcaaagc caattggtag gagtcaccga
     5701 ttttaccccc aaactgttca attaatcgaa caatttctcc acccatataa taatgggcaa
     5761 gaagtaacag tttgttaccg aaatgatttt gggctttagc aagataaggt ttcatccaat
     5821 ttaaaacagt cgtaagcttt ctatcgggta aagcctgata ttcctgggca taaggaataa
     5881 aatcttcttg ataccaatct aatggataat cgctttgaca tatagccata tcattacgaa
     5941 tagccatgag ggtcgcttcg ggttggtaag tagttgaatt tttaaacatt aaatctacct
     6001 ctagtcatta gggatttgtg ggaaggattc tttcattatt gttaatggga tagctttgcc
     6061 aggctatcct aatgaactgt ctgcctgtcc cagttttgca atactttcct gggctttgaa
     6121 acttttattg gggaaatctt cacgaaaatg tccaccacgt gattcccttc tggaaagggc
     6181 tgctctaaca atcaattctg cattaaggat aatgttcctt aactcaataa aatctctggt
     6241 aatgcagtgt ttccagtaat actcttcaat aattttctta cgcttcaata ttaattgtag
     6301 tagatcttca aggcctgctt cagtgcgcac aatgcctgca taggaagtca tttcccctct
     6361 taagcctctc cattgagcgt taatttggct ggctcttctt gcattgactt cacctggaga
     6421 gctccagcta gggatatgat cgataagttt cagcggagta gcaatgtctt tgagtgtaca
     6481 gcgtgccgca ttggaaccca tgaccaaggc ttcaagtagg gaattgcttg ccagcctgtt
     6541 cgcaccatgc agcccagtaa aagccacttc accgatagcg tataatcgtg ccagatcggt
     6601 gcggccgtcc acatcagtaa gaaccccacc gcattggtaa tgagcggccg gaacgacagg
     6661 aatcaaatcc tggctcatat caattccaat agataacaat gtggaataaa tttgtgggaa
     6721 acgttttttc aggaaagatt tgctttgatg agaaatatcc agatgcacat atccagcctg
     6781 gccttgctca atttcactaa aaatagctct tgccacgacg tctctggttg ctaactcaag
     6841 ttggtcaggt gcgtatcgtt tcataaaacg ctcgcctgtt tcagggtttt taagcaaagc
     6901 gccttcgcct cgaactgctt cggaaattaa aaaattgtta atggtgtgat ggtgcaagag
     6961 ggtaggatga aattgataaa attccatgtt gcccacacga gccccagccc tgtatgccat
     7021 cgcgacacca tcacctgttg caaccatagg gttagtcgta tacctgtagg ttttgccggc
     7081 gccaccggtc gctaaaatga cgcatttcgc aagaaaggtg tgaatgcgat tatgttggca
     7141 gtcgagtata taggctccta atacctcacc ctggttatct gtccggtgag gatgatattg
     7201 agtaatcagg ttaaccgcca cgtgatgttc aaaaaactgg atctgttgat gttgccttgc
     7261 caaatgattg agagtttgtg ttagcgttaa tcccgtttga tcgccacaat taaaaatgcg
     7321 ccggtgtgaa tggccacctt ctttggcaag atgaaagcga ccatcatttt ctcttttaaa
     7381 ttgaacttta tactgatcaa gttgttttat aatatctggc ccttggcgga taataaattc
     7441 caccgcaggt aagtagcata gcccatctcc agccaataca gtatcagata tatgagactc
     7501 cagtgaatct tctggcgaaa tggcggcagc aatcccccct tgagcatagc ggctgttaca
     7561 ctcaatggac tctgctttgc tgattaacgc aatgtttagc ttgggttgga tagctaaaag
     7621 ctgcaaacaa taatgtaacc cggctaatcc tgttccaatg acgagaacat cgaattcgaa
     7681 cgattggcct tgttgtgata gctgatcact catgaaaaag ccttgaccaa ttgggttgca
     7741 agacctgtgt aagtctctgg agttaatttc attaactctg cttttgcttc ttcgggaata
     7801 gacagggttt taataaattt tttaaggttt tcagcgtcta tcatttgtcc tcgtgtaagc
     7861 tcttttaatt gttcataggc attgggttca ttgtagcggc gcatgacagt ttgtatggcc
     7921 tctgctaaaa cttcccagtt ttcggataaa tctttttgta gagctgattt attgatttgt
     7981 aatttatcat tacctttggc tactgagtga taggcaatca aactgtaaga gaaggctacg
     8041 cccaagttac gtaagacagt cgaatcagac aggtctcttt gcatgcgtga ttgagttaat
     8101 ttgttggcaa aatgaataaa taaagcatta gacagaccta aattgccttc ggcattttca
     8161 aagtctatag gatttacctt gtgcggcatg gtcgatgagc ctacttcttc ggcaattgtt
     8221 ttttgcttaa agtagccaag agaaatatag ctccatatgt cttgggtata atccagaaga
     8281 atattattaa ttctcaccat aatctgggag acttccgcta tgccatcatg tggttcaatt
     8341 tgagtggtat aggcgttaaa ggataaacct agggatgtaa caaaattagc gcaatgctta
     8401 cgccagtcca cttcgggata ggctgctacg tgagcattgt aattaccaac agctccatta
     8461 aatttggcag ggattaatac ttctgccaat tgttgctggg gtcgtttaag gcgagctaca
     8521 aaattgacca attctttacc catggtggtt ggtgttgctg gttgaccatg cgttcttgag
     8581 agcatcgcaa cgtcagcatg ttgttttcca agaagcgtaa tgcttcccat gatctcagct
     8641 atagtaggtt gtataacttg ggctatcgct tgtttgatca ttaaagcata agccaggtta
     8701 ttgatgtcct ctgaagtgca ggcaaagtgt atgaaagcaa cgcaagattt gagttgttca
     8761 ttttcctgaa atttatcctg aagataatac tctacagctt taacgtcatg attggtttgt
     8821 ttttcaaatt cttttatttt ttcagcttct gattcattaa aattggaaat aagatcagat
     8881 aaaaatttac gagccttgtt atccaaagca ggtacttctg gaatagtgtc gttagcagct
     8941 aatgattcaa accacttgat ttcaaccatt aaacgataat aggttaatgc aaactcacta
     9001 aaatagggac ttaatgctct ggttttattg acataacgac catctatggg tgaaatcgcg
     9061 tttaatgcag taagtgtcat gataggtagc ttattccaat attaaagcgt atataatatt
     9121 agatgcagga caagatgtga attaaagcga taaatattct agaaaattag cgtaaatttg
     9181 ttcaaagtgc gtataataat gaccatgttt ttaatgggac aggttattat ttatgaacac
     9241 gatcacagaa tcgtatcgag ccggtttatt tcaaaaaaaa tattggttac aaaatattat
     9301 ttctggctta attgttggtg ttgttgcttt acctttggca atggcttttg caatagcttc
     9361 cggggctaaa ccggaacaag gattatatac cgctatcgtt gccggattaa tagtctcaat
     9421 tatgggtggc agcagagttc aaatcgccgg tcctacaggt gctttcattg tcgttctttc
     9481 cgggataacg gcccaatatg gtatttcagg gttacaaata gctactttaa tggcgggctt
     9541 tatcttggtt ctgtttggct ttgcccgtct gggatccatt attaaattta ttcccagtcc
     9601 agtaatcatc ggatttactt cagggattgc tgtcgttatt tgggtagggc aatggcaata
     9661 tttttttggt ttacccgcag ctggaagcgg acattttcat gaaaaatttt ggcatttact
     9721 tctgtctttt cctcatatga atatcgcgac tactttttta ggtatttgtg ctttatttat
     9781 tgtactgtat tccagtaaat taccaggact aaagcgtatt cctggccctt tgattgcttt
     9841 ggtactggtt acaatcatac agtcagtttg gcactttgag ggagtggcca ctataggcag
     9901 cctctttgga ggaataccac aaggcttgcc ggctttcacc tggcctgaaa tcacaatggg
     9961 acgactgatt gagttgattg ggccagcctt tgctattgcg atgctgggag caattgagtc
    10021 cttattgtct gctgtggttg ccgatgggat gagtggaacc agacataatt cgaatagaga
    10081 attagttggt caagggatag ctaatattgt ggcgcctttg tttggtggtt ttgctgccac
    10141 cggagcgatt gcccgcacgg ccacaaacat acgcaatggg ggcaatagtc ctctggcagg
    10201 aattattcac agcattactt tgattttagt cctgttgttc ctggctcctc tggcagtgga
    10261 tgtacctctg gcggcattgg ctgctatttt atttgtggtt tcgtggaata tgagtgaagc
    10321 ccgccacttt attaaattaa ttcgagtagc tcctcgtgct gatgtagtca tcttattggt
    10381 tactttttgt ctgacggtgt ttgttgattt ggttgttgct gtcaatgtag gggtcatcat
    10441 tgccgtcttg catttcctgc gccgaatggt taacagcatt caaattcagc gtatgtcaga
    10501 aggacagtta tctcaagagt tggcacaaga aaagaattat gatttgcccg aaggggtcat
    10561 ggtgtatgtt atcgagggcc cgtttttctt tggtgctgtc gaaacatttc agagtgcgtt
    10621 ggctaatacc catactgatc cgaaagtttt gatcatcaga atgcgttggg ttccgtttat
    10681 agatgtcact ggtttgcagg ctatggaaga aattattttg aatttgcaga agcgtggagt
    10741 cagagttttg ctcagtgagg ctaacccgct ggttgatgcg aaactccgta aaatgggaat
    10801 tgttcaattg attggagagc aaaattatta taaggaattt tctcaggctc ttgatgtttg
    10861 ccgaattaat gattctgaca gaaagagtca gcctcccttt gaagttgcaa agccgctggc
    10921 ttcagttacc cagtgagcta agctggttat ctgttcaagt tgaggaagcg cttcctcaac
    10981 tcgaatggat agaattaacg tgataatcga aaaacttcaa agattgccat gcctgttttt
    11041 atatgtaatt caatttgcta aacttaaaat ctattttttt ggtttgacac tacgaataat
    11101 ttttcccggc ctgctggtta atgcatttgc agagtgaact atccaattac tttatttagt
    11161 attttcatat agtcgtcgtg ttgaagtaaa caacttgcta tatgatcatg aataaataaa
    11221 gttaatccag cttgttttgt ttctggcgac tggccttgtt gtatctctat agccagacat
    11281 aaagcagcaa gcaacgaggc catatgttca atgattgttt ttatatgcag actttgctcc
    11341 tcatgattgg ctaatttcat tgtttttatt gttgtatcta actcatttat cttttcgaaa
    11401 aacaatgcct tatgagggtt gatatcctga acttgttcca gaagttgttg caaatgggca
    11461 atgaataatt catcaattgc aaatttatgc atatctctga gggtttgcat ccataatgta
    11521 aaatgcgtcc cctcccaatt ttcgcaaacg atacagtctc ttaatagtct tggtaatgaa
    11581 gaaaagttct ctatggtgcc atttcctgcc aataaatcga gacaatgatg tatattgtcc
    11641 actgaacgtt tggctgtaaa gtacttgttg atattcgcca gtgtacgtaa taagagttgt
    11701 tgtgatttgg tttgttgagg ctctggaaga gcatctaatt catcctgaca acgagccata
    11761 tgaaacacgc tggctaacat agccagattt tctgatttta ttttagccaa actttctttt
    11821 accagaggat attcaataat aggatggtta aatgctcggc ggtttttagc ataatagcaa
    11881 gcaatctgat aggcgcgacg agacataccc aagactgaga aggaattgaa tatccttgat
    11941 aaatgcaaca cattttccat aaccaaatgt attccttccg ccacatcacc catagggtag
    12001 gctagtgccc cttcaaaatt tatttctgcg gttgccatgg tgcgggtacc aattttttgt
    12061 tttaatcgac gaagataata gtgattaggg ctgccatcgt ccaggcgact gggcactaaa
    12121 aacaaaccca accctttggt tccgggagtt tcctcgtcga aacgagccgt cattaaaatt
    12181 aaatcggcat tagcgttgga gcaaaaccat ttttcacctg aaatgcgcca ttgattttgc
    12241 ttatccagac tggcacgggt cgcattggcg ccaacgtcag aaccaccttg aatttcagtt
    12301 aaaaattggg ctccagtaaa attgtaactg aaagaagggt gagtgagttt atcgagataa
    12361 tatgaggttt tctctaattg ggaataacga cttaaaacac gtattactcc cgcagaacag
    12421 gcaatagggc agttgtgtcc tgcctccccg gcatgcgatg agagcagaaa caggctcaac
    12481 gtttttttca tttgccctgg ctttagtaaa tattgcatga ggttggatcc ataaataata
    12541 tcgccggatc gaacataggc aggatggtgg attacccgat catcgcgctg accaattgca
    12601 ttgtattgtt cgatttttgg caagttggca tccaggttat tttccataac ggttggttct
    12661 aattcttcag ctactgcttt ggaaaattga ctcaattgtt gaataaaacc aggctcatcg
    12721 ggaaaataag ttttgtaagt gtgcattaaa caagactttt cgagcagtat attgtctttg
    12781 agttgtttgt cccataacct gaaatggtct cttgctgttt catattcatt ttttatgctc
    12841 attaccccac cctttggtaa ttaatagatg cattgatatc acagtttttt aagttcttgt
    12901 tgtacttaaa tatttttcaa taaattgtaa tagcggttat taatcaatta tccccctcgg
    12961 cttcgtttgt gccatatcca tgcggcttca caatagacct cttgcctaaa cttgcctctt
    13021 tcattaaaca aaataatcag gttctgcaag agggcctaat gaattccaat ttatcctatc
    13081 atgggttgaa atttatcagt gacgtccccg gacaaatgtt gataaacatt cagggcgatt
    13141 ggttgataaa gataatcaaa gtcaatgcta tccattggaa tcactcttaa tgcagcctcg
    13201 ttaatgctgc ggaagtaaat tgttttggtc gggatatcaa aaaccatact cagttgggtt
    13261 ggagaagaag tgccaggtgc ttgtgtgacg acattgaggc cgttgaaagc ataagcaata
    13321 tgttcttctt tagctataaa gctgggaagt ttttttagat aatgtgctgc tcttacaaaa
    13381 cgggatttag aatcatatcc acctggcaga gccttggagc caccatggtc ctggtattct
    13441 tcaagaagag ccaatgacga tttatagtcc gtattggtta gtactggcgt ggtcaagttg
    13501 tcttgagtat gaattaccag tttaccatcc aggtactcaa aaacagcaga ttttccttcg
    13561 gcatcgctca ccatcaagtg aacgttcatg gttattcccc ggtaggtgtc tgcaaccagt
    13621 tgataatttg agctgtcatc tatgacttct tggaccgttt gaaaattatc taaaatatac
    13681 tgaacccatt cagtagtttt taaagcggga agctcagagt tactgggata acttgatgaa
    13741 tcaagcatca gtattgaagc agtaagtcct ttttcattca tcccatcagc agatggtcct
    13801 gctttaaacc gatttcctcc gtgaaatatc acacttccat attggctttt ccagattaat
    13861 ggatgcatgc tgtggtaatc tacactggaa accatcatgg catttcgagg atgaatcaca
    13921 aaagctcctt cccggtattt ccagtccaaa ttgactgcaa cagttgcggg cttgttttta
    13981 ttgaacacta aggcagaaca ggcttcgctg gttaaggaga tagtatttaa agataaggca
    14041 atcgatatta gtcctatgcg tttaaacatt tcaaatcctt ttgcttatta tattcttgga
    14101 acaggtttgc ttacccgttc aaccctaaaa catggacagc aagatatctt accgcatagt
    14161 acatatactt gtcatttttt gtgaaaatgc cggtattttg acctttataa aaatgaaata
    14221 cagcacaagt tctaattcca ataaagtaac aatttaagta gaatgtttac tattttatga
    14281 atttggggcg gttatgactg ttaaaagaca tactatagat ttggcacatc ttggcatgcg
    14341 cgatttggat caggtcggtg gtaagaactc ttctcttggt gaaatgatta gtcatttgtc
    14401 atcagcaggc gtttctgtgc cgggaggatt tgctacaact gctgattctt tcagagaatt
    14461 tttgtcccaa aacggtcttg ataagaaaat ttatgataag ttgactgctc tcgatacgga
    14521 tgatgtctgc caactggcag tggtagggaa acaaattagg gaaatgatta ttgatacgcc
    14581 attcaccccg gagtttgaac agtcggttcg ctcttcctac cagaaacttg ctcaagagat
    14641 tgggcatgat aattttagtg ttgcagtaag atcttctgct actgctgaag atttgcctga
    14701 tgcttctttt gccggtcagc aggaaacttt tttaaatgtg aagggagaag aggcggtatt
    14761 ggctgccatt aaacaggtat ttgcttcttt atttaatgac agggcaatag cttatcgtgt
    14821 gcatcataat tttgctcata acgaagtggc attgtctgct ggcattcagc aaatgattcg
    14881 tagtgatttg gcagtcagtg gtgtcatgtt tactatggat actgaatccg gttttgatca
    14941 agtagtattt atcacttcct cttatggttt aggtgaaatg gttgttcaag gcgctgtaaa
    15001 tccagatgaa ttttatgtcc ataaaccatg ccttgaagcc ggaaaaccgg cgattatccg
    15061 tcgaaacctg ggcagcaagg cgttaaagat ggtttattgc gatgaccctt cccttgaaag
    15121 acgtgttaaa acagtcgatg tagaccctgc tgaaagatta ttgttttcct tatccaaaga
    15181 ggaagtagag caattagcga atcaggcact tattattgaa aaacattatg gtcgccctat
    15241 ggatattgaa tgggcgaaag acggggtcaa tggtaaactg tatatcctgc aagctcgtcc
    15301 ggagactgta aaaagcaggg ataacaaaca agtgctcgaa cggtatactt tgcaaaaaag
    15361 aggcgacgta ttggctgaag gccgtagtat aggtcaaaga atcggtcagg gtaaagcaaa
    15421 ggttattaaa gatattaatg aaatgcacag ggttcaaccc ggcgatgttt tgatatccga
    15481 tatgaccgat cctgattggg aacctgtcat gaagcgtgct tctgcaatcg ttaccaatcg
    15541 tggaggcaga acttgtcatg cagctattat tgctcgtgaa ttggggattc ctgcagttgt
    15601 tgggtgtggt gatgcaacaa aaacgattaa agatggtgat gtggtgactg taagctgtgc
    15661 ggaaggcgat acaggttttg tctattctgg cctgttgcct tacgagcaag agcgtcttga
    15721 tgtagaaacc atgcccgaat tacccatgaa agtgatgttg aatgttggaa accctgaaag
    15781 agccttcgca tttcaatcaa tacctaattc cggagtggga ttggcccgtc ttgaattttt
    15841 gatttccaac acgataggta ttcatcctaa agcgcttctg gaatttgata ctttaaagga
    15901 tgaggaactt aagcggtaca ttaaagaaaa gaccatagcg tatgattcac ctgtagaata
    15961 ttacattgaa agattaaaag aaggcatagc gactattgct gctgcttttt atcccaaacc
    16021 cgttatagtc aggctttcag attttaaatc gaatgaatac gctaatctgg ttggtggctc
    16081 attgtatgaa ccccaagaag aaaatcctat gcttggtttt cgtggcgcct ctcgttatgt
    16141 ttcttcaaat ttttctgagt gctttgctct tgaatgtaaa gcagtaagac gagtcaggga
    16201 ggagatgggt ttagataatg ttgaggtcat gattcctttt gtccgaacag tttctgaagc
    16261 cggtaatgtc atcgaagtat taaaaaagca tggtctggaa agaggaaaat gtgggttaag
    16321 agtgattatg atgtgcgagt taccttccaa tgccttgtta gccagtgaat ttttgcagta
    16381 ttttgatggt ttttccatag gatcgaatga tttaactcaa ttgactttag gattagacag
    16441 ggattcaggt ttggtggctt cacaatttga tgagcgcaac gatgctgtga aagctttatt
    16501 gcatatggct attgcagctt gtaaaaaaga aggcaagtat ataggtattt gcggtcaagg
    16561 tccttcagat caccaggatt ttgcccaatg gctgatgaag gaaggaattg agagtgtttc
    16621 tctaaaccct gactctgttt tacaaacatg tttgttttta gctaaacaat cgatgaattg
    16681 atgaaccaga gtgtactggc aattcagttt gaaattgtta ggctgttggt gcattaggaa
    16741 tttactgagg cattcctatg attttgaaat gcagcagatt gccttggaag atgagttttt
    16801 tgatcaatac aattattttg gctgttagta atggaactac ttgtgatggt atggtgttgc
    16861 aattcaaaat tcccttgcat ttgggagaaa gcagcacatt aagcttatag gataaagcgg
    16921 tatgagtaat tcaaaatggt ggtttctttt agtctgtttc attcctgcta ttacttttgc
    16981 atctgagggg ggagagcatt cagatccagt cacctctgtt cttctgggtg tcactactat
    17041 tttattttta gctatcattg gtcgttattt agccagactt cttaatcaac ctggcgttct
    17101 tggtgaatta ctgataggga tgatagttgg gaatctctct tactttttcg gaagtgaatt
    17161 agtagtaatt cttcgcgaag gttctgccat ttatgatgtt gtcaaagaaa tgttagcagg
    17221 aatttcttta aatcaggcgg taactcatgt tatccataat tcctattatg ctaaccaggt
    17281 gatccatgcc ttaagtggag cagagggaac tgaatatctt aaaatagcct atgtcgttga
    17341 tattttctca cgttatggtg tcatatttct tctttttatg gttggtcttg agacgtcgct
    17401 ttctgatttg aaaaaaactg gcaaagaatc cattcttgtt gctgtgatcg gtattgttgc
    17461 gccaatgatt ttagggtttg tagtggcttg ttttatcata cctaactcaa attacaagat
    17521 taatttattt atcggcgcta ccttgagtgc taccagcatt ggtattacag caagtgttct
    17581 taaagagatg aataaactga gtacacggga agcaagaaca attcttggtg cggctacttt
    17641 ggatgatatt ttaggcctga ttattttggc ggtagtcagc agtatcgtta tcagtggtgc
    17701 tgtaagtatc atggttgtaa tgaaaattat cattttagcc ctactgtttt tcattggtac
    17761 cttgtttatg gggccttttg tacttcggaa ggcagtcgat ttattccgtt ttctggagcc
    17821 ttgggaatcc aaattattta tctcattcct ttttattatg gcattagcct ggttggcttc
    17881 atttatacag ttggcggcta taattggtgc ttttgctgcg ggcgtcattt tgcacgacga
    17941 ctattttgga gagcaaaaag cccatcataa agatcatcgc agtattcatc atttggtatc
    18001 tcccttggag actatattgg ctcctatgtt ttttactctc attgggatac aggttaagtt
    18061 agaaactttt tatgattgga atgtcattgt tttagcggga ggcttatcag tagcggccat
    18121 tcttggcaag ctgcttagtg gcttaggcgc taatcggcgg gatgataggc tgctcattgg
    18181 tataggaatg ctgcctcgtg gagaggtagg tttggttttt gcttctattg gcagaactat
    18241 aggcgtaata agtgataatc tgtttaccgc aattgtattg atgatcatga tcacaacctt
    18301 tattgcgcca cctttattaa agatgcgata tgccaaacca aaagaggata ggaatgcatg
    18361 aataatgttt tatcgcaagt tacatttgat gtaaaacgtg ctttgcaaga ggacgtgggc
    18421 aatggtgatg ttactgcggc tttgttgcct cagcagctga ttgtagaagc agaaattata
    18481 tcaagagaac ctatgcttgt ttgtgggcag ccctgggtaa atgaggtttt taagcaagtt
    18541 gataatacag ttgaaatgga gtggcgggtt tctgaaggag attttttaag cacgccagct
    18601 actttatgta ttattcatgg tactgccagt agtattttaa cggcagagcg tactgcgtta
    18661 aattttttgc aaaccctttc agctacggca acacaaactt atcactatgt tcagaaatta
    18721 aaaggtacca aggcaaagct gttagacaca cgcaaaacaa ttccaggact tcggttagcg
    18781 caaaaatacg ctgtccattg tggtggcggg ttaaatcatc gtatgggact ttatgatgcg
    18841 tttctaatca aagaaaatca tatcaaagcc tgtggttcag ttgctcatgc tatcagtctg
    18901 gcaaggaaaa ccgataaaca cttactggtt gagattgaag tggaaaccct tgaggagttg
    18961 caagaggctt tggatgctca ccctgacagg atattattgg ataatttcac tcaagatatg
    19021 cttgagcaag cagtcaaaat gaatcatccc aagtactgtg aattggaagc ctctggcggc
    19081 attaatatca ataatattgc agacattgct caaaccggag tggattttat ttcagttgga
    19141 tccatcacca aatcaattca agcaattgat ttgagcttat tgattagaga aacattatga
    19201 gcccaagtat tgtttttacc gggggaggca ctgccggaca tgtaacgcct aatatcgctt
    19261 tgattaagga atttcgaaaa gaaggctgga atgtagaata tatcggctct gtttccggaa
    19321 ttgaaaagga gatgattgag ccgatggaca ttccttttca tggagtcagt agcggtaaat
    19381 tgcgcaggta ttttagtttg aagaacttgc ttgatccttt caaaattgtt ctgggaatta
    19441 ttcaatcttc tttgctattt tataaaatca aacccgatgt ggttttttca aaaggtggct
    19501 ttgtagcctt tcctgtggtt gtaggcgcct ggttaaatcg aattcctgtt gtcgctcatg
    19561 agtctgatat gagtccagga cttgcgaatc gcctatcctt tcctttcgtc aataaaatat
    19621 gccttacttt tgatgctggc aaaaaatact ttaagcgtca ggataaaata gaagtgacgg
    19681 gtactccaat tcgtcaacag ctattaactg gaaatcgaat gaaaggattg gagctatgcg
    19741 gatttaattc ctccaaacct tgcctgcttg tagtgggagg aagcttaggg gctggttcaa
    19801 ttaacagttg tattcgaagc gcattgaaac aattgacatc agaatttcaa gtcattcatc
    19861 tttgtggcaa gggaaaactt gattcttcat tagttggtgt ggagggatat tgccaatttg
    19921 aatacgccaa tgaagagttg gctgatctgt tcgctgcttc ttctgtggtg atttctcgag
    19981 caggagctaa ttctttgtat gaaatattag cattaggaaa accacatatc ttaattccaa
    20041 tctcttcaca agtaagcaga ggagatcaaa ttcagaatgc aaggtacttc cagggattgg
    20101 gaataagcgt tgtgattcag gacgagttat tgaaagctga tgttctatta caggcagtac
    20161 aggacgtaat gcgaaaaaaa gatgaaatag ataataaaat caaagcatta aaaattgagt
    20221 ctgccactga taagattgtg gcaattatca aggagcaagc acatgttcaa accccaagga
    20281 ttgtatgatt acatatgcca acagtggcaa gaagagatat tgccaagttt atgtgactat
    20341 ataaaaatcc ctaataaatc tcctcacttt gatgcaaaat gggaagaaca tggttatatg
    20401 gagcaggcag ttaatcacat tgccaattgg tgtaagtcgc atgctcccaa aggaatgact
    20461 ctggaaattg ttcgcctgaa aaataggact ccattactat ttatggaaat tccaggccaa
    20521 attgatgaca ctgtgttgct ttatgggcac ttggataagc aacctgagat gtcaggctgg
    20581 agtgacgatt tacatccatg gaaacccgta ttgaaaaatg gattgttata cggaagagga
    20641 ggagcagatg atggatattc tgcttatgca tcactcacgg ctattcgcgc cttggaacag
    20701 caaggtttgc catatcctcg ttgtatatta atcatcgaag cgtgtgagga aagtggcagt
    20761 tacgatttgc ctttttatat tgagttgctg aaagagcgta ttggtaaacc atcattggtt
    20821 atttgtcttg attccggagc aggtaattat gagcagttat ggatgactac gtcattacgc
    20881 ggtaatttag tcggtaagtt aactgttgaa ttaattaatg agggcgttca ttcggggagc
    20941 gccagtggta tagtggcaga cagtttcaga gtagctcggc aattgatcag caggatagag
    21001 gacgaaaaca ccggagagat aaaattacct cagttgtatt gtgatattcc tgatgagaga
    21061 ataaaacaag cgaaacaatg tgcggaaatt ctaggtgaac aagtttatag cgaatttcca
    21121 tggatagatt ctgccaaacc cgttattcaa gacaaacagc aattaatatt aaacagaaca
    21181 tggcgacctg ccttgacggt gactggtgca gatgggtttc cagcgatagc tgatgcaggg
    21241 aacgtaatgc gccctgttac gtctttgaaa ttatccatgc gtcttccacc actggttgat
    21301 ccagaagcag cttctgttgc tatggaaaaa gccctgaccc aaaaccctcc ctataatgca
    21361 aaggttgatt ttaaaataca aaatggaggt tccaagggat ggaatgctcc tttgctttcc
    21421 gattggttag cgaaagcggc atctgaagca tcaatgactt attatgataa acctgctgcc
    21481 tacatgggag aagggggaac cattccattt atgagtatgc taggcgagca atttcccaaa
    21541 gcacaattta tgataactgg tgttttaggc ccccattcca atgctcatgg tccgaacgag
    21601 ttcttacatt tggacatggt aaaaaaactc acctcatgtg tctcgtacgt tctttatagt
    21661 ttttcacaga aaaaataatt tgaaattggc tagactcttg tttgaaaaat tgagatactc
    21721 gcttgcggct ctggaacgag ccgtttagtc caacaattag gagaaattta tgaaagcaag
    21781 actcattgct attttgttgt catttattgt tatccctttc tcttctcaag ctgagaacga
    21841 gcctcaaaaa actccagcta aacaagtcct tgttaagggc aagattaatc tcaatacagc
    21901 ggatgtgcat tctttaacag gctcttttaa agggatagga aaaaaacggg ctgaagctat
    21961 tattgcttat agagagaatc atcaaggctt taaatctctg gaagagcttg cggaagtcaa
    22021 gggtataggg cagcgttttg tagataaaaa tagagaaaaa ctgaaagaaa ttttcgttat
    22081 taattaattc acgtgcaata taaaacgtaa atcatgtttt ataaagcaaa tgagattgat
    22141 cccatatgaa agcgttgctt ttactatggg atccggttaa aagaagttgt ttggtgtata
    22201 aaattcttat gcggcaactg tcgattttta tccaaatata ggataaagta gcaatcaaaa
    22261 aaacaacggg gtagtactgc gtatgttcag gaaattaaga ggcgtgtttt ctagcgattt
    22321 atcaatcgat ttgggaacag ctaatacatt gatttacgta agagataagg ggattgttct
    22381 caatgaacct tctgtagtgg cattgcgtaa tgaatcaggc cagaagcgtg tcgccgctgt
    22441 aggtcttgaa gccaaacgca tgttgggaag aacccctggc aatattaatg cgataagacc
    22501 catgaaagat ggggttattg ctgacttttt tgtcaccgag aaaatgttgc agcactttat
    22561 acacaaagtg catgaaaata aatttttacg acccagtcca cgcgttttgg tctgtgttcc
    22621 ttgtggttct acacaagtgg aacgcagagc aattcgtgaa tctgcaatgg gcgctggtgc
    22681 tcgagaagtt ttccttattg aagagcctat ggctgcggca ttaggttccg gaatgcctgt
    22741 tgaggaagcc agtggttcta tggtggttga tataggcggt ggtaccacag aagtcgcaat
    22801 tatttcacta agtggaatcg tttatcatca atcagtacgt attggtggcg ataaatttga
    22861 tgacgcaatc gtgtcctacg tacgccgtaa ttatggtact ctcatcggag aaacaactgc
    22921 tgaacgtatt aaacatgaaa ttggttcggc tttcccaagc cgggatttat ttgagatcga
    22981 ggtacgaggt agaaatcttg cagaaggagt accacgtagc tttactttaa caagcgctga
    23041 aatcctggaa gcactacagg aacctctatc agggatagtt ggtgcggttc gagctgcttt
    23101 ggagcttgct cctcctgagt tggctgcaga tattgctgaa agaggtatgg ttttaacagg
    23161 cggaggcgct ctgttgaaaa atatagacac cttgttgatg gaagaaactg gattacccgt
    23221 attaatagct gaagatccat taacctgcgt tgcgcgtggt ggtggtaaag ctttagaaac
    23281 catggatttg cgcggcggtg atttcctctc aacagaataa taaacacaat agattatttg
    23341 ctaagggcgg acacagttct ctgggatttg tgtttgccct tgctttttct attctcctga
    23401 tgttttctga ttatcattat cgatacctgg atttcgttcg tagcggtttt tcactaatag
    23461 tttctccctt gcaatatgcg gtagattatc ctgttcgagt catcggctgg attcagtcgt
    23521 tggttagtgc caaaaaggca ctgatagatg aaaatatgcg tttaaaatac aagcaaacaa
    23581 tgcttgaagc tgaattgcaa aaattaattg ttatccaaag agagaattcg caattaaaag
    23641 aattattatt gacttcatcc aaggctgata tgagagtcat ggctgcccag atactcgctg
    23701 ttgatacaag tcaggcaaga caagttgtcg tcttaaacaa aggcacacgt gatggcgtct
    23761 atgtgggaca gccagtgttg gatgccaagg gagtgatggg gcaggttatt gatgtaggac
    23821 caatgacaag cactttatta ctgatttcag attcaaaaag tgctgtgcca gtaagaaaca
    23881 atcgaaccgg agaacgcgca atccttgtag gcactaatga tattgaagaa ttgtctttga
    23941 ttaatttacc gaagacttct tctattcatc caggtgatgt gctggtaacg tccggtttag
    24001 gccgaagata tccggagggc tatcctgtag gacgcgttga ggaagttaag agtattcctg
    24061 gcgaagattt tgtcaaagtc acagttagcc ctgtcgcatt gttgaatcgc aacaggttgg
    24121 tgttattgat ttgggctgat agtgagcaag aggaattaac tgctcaaatt aatgagcgat
    24181 tgaatgctga ggagtctaca gcatgagcgt gcttcacatt cgtttaggat tggggtttct
    24241 cgtggcactt gctttatcaa tactgccgat gcctgagtta atttcaattt ttcgacctcc
    24301 ttgggtgtta ttattggttt tatatattga atattattta ccaggtaaat ttaaactgac
    24361 tgctctgttg atagctggct tattactgga tgtactctta tcaaccgtga tcggtgaaca
    24421 ttcattcgca ttactattgg taacctggat agcgtcaacc aaatcgagac gagttcaatt
    24481 tttctcacta attcagcagg tttgtttgat tggatttttt tgttttctat atcaatcaat
    24541 tattatattt attgatgctc tgttaggttt taattataac ctgataatgc ctgtagcaag
    24601 tgccattttg ggtatgatta tttggccctg gatccgagtc ttgggttact cttccttgtt
    24661 aactcgttca gtttatcgtt agtaaggttg attaacttat attcccctgg ctgaagttga
    24721 tctaaccgcc aatcaccaat ctggtgtcta attaaacgta aagtaggaaa tccaatagcc
    24781 gcggtcattt ttcttatttg atgatttttc ccttctctta aaataatttc cagccaggta
    24841 gttggtatgt gtttccgttc tcttataggc ggaattcgtg gccatatttt tggctctgaa
    24901 atcaacttgg cttcggcggg taaaaaagta atgtctttga ttaccaggcc ttttcttaaa
    24961 ggttgtaaat ctttgtctgt aggggccccc tctacttgaa cccagtaatg tttctttttt
    25021 tcaaatttag gatgggttag ccggtgttgt agttttccat cgtcagttaa gacaagcaag
    25081 ccttcgcttt gtttatccag acgtccagct gcataaaaac ccggaaattt aataaatgcc
    25141 gagagagttt gttcgggtaa ttcgccagta aattgagtca ggactccata aggtttatta
    25201 aaaagtatta attgtggcat tttatgattg cgttgattat ggaaattaat gataaatttt
    25261 ttggttattg acagcaacca cttcagggct agatcacgcc tttaagcaca ataacagcgt
    25321 aaataattgt tcatattttg tcattcactc aatgagatat gaggtgcagt aattaatcaa
    25381 aatatgattt tgtccctctt ggaattaaag ccaagattga ttttttatgg ccaatcacta
    25441 tacaattaca caattttatt cgttatggtg actcttattc atgaaatggg tattggttgt
    25501 tatggcaatt atcgctcaaa caagttgtgc agtgaatcac agagttatgg ttggggatga
    25561 acctgtaata attcaacaaa ccaaaggtca aggtaagact tttgtacatg ttcatcacaa
    25621 tgaacaaacc gcactaaaag ctgctaaagc tgtcattaga aaagagggtg gaagtctcat
    25681 tacacttatc cattccggcg gtcgaaatat agtattccat ttacataacc aacgctatga
    25741 atttgatccc aaccgcattt ttaccgacaa agggataaaa aaaactttga cgcaatacag
    25801 ttattatact cctgaagcgc atcaagaagt gaagaaactt gcagataaaa ttaaggcctt
    25861 actaccagag ggtaaagtaa tcgctgttca taataactcc tcctattctt taaaagatta
    25921 tttgcctgga catgaacttg ccaaggatgc aaaagctttg tatatgaatc ctgacaatta
    25981 ttttaggaat ttttatctgg ttactaaatt aagcgattat ctgcgtttaa aaatgcaagg
    26041 ttttaatggt gttttacaaa aaccttcggc tacagacgat ggatctttat cagtctatct
    26101 cgctaaacgt gattatatta atgtcgaggc cggttacgat cagttggcag aacaaattaa
    26161 gatgttgctt catgcttagt ttcaatctga gctaatttag caaaaaaaaa ataattaata
    26221 gtcgtaattg atatttggga aacctgatta tcacatgcta tcattaattt ctctaaattt
    26281 atggagtcgt caatgacata cgataaaatc aaagtgcctg cccaaggcga agctattact
    26341 gttgctgctg accattcact tcatgttcct gataatccaa ttattccttt tattgagggt
    26401 gatggtatcg gtgttgatgt aacacctccg atgatccgtg ttgttgatgc tgcagttcaa
    26461 aaagcatatg gcaacaagag aaaaatttcc tggatggagg tttatgccgg ggaaaaggct
    26521 actaaggttt atggtagcga tcaatggctt cccaaagaga ctctggatgc catgaaaaaa
    26581 tatgtggttt caattaaagg tcctttaact actcctgttg gtggcggtat acgttcattg
    26641 aatgtcgcta ttcgccaaga catggatctc tatgtttgtt tacgtccaat tcgctatttt
    26701 aatggtgttc caagcccagt gcgggagcca tggaaaaccg atatggtcat tttcagggaa
    26761 aattccgaag atatttatgc cggtattgaa tggcaggcag ataccccgga agctaaaaaa
    26821 gtgattcaat ttttaactaa ggaaatgggt gttaagaaga ttcgttttcc tgagcattgt
    26881 ggtatcggtg taaagcctgt ttccagagaa ggtactactc gacttgtcaa agcggccatt
    26941 cagtatgcga tagataatga ccgcagtaca gtgactctgg tacataaagg caatatcatg
    27001 aagtttactg aaggggcttt taaagattgg ggatatcagg ttgctcgcga ttcttttgga
    27061 gcaaaagaat atcaaggtgg gccatggatg gaatttaaaa atcctaaaac cggtaagcag
    27121 attatcatta atgatgtgat cgctgatgcc tttttgcaac aaatcctgtt aagacctgaa
    27181 gactatagtg tcattgctac gttaaatttg aatggtgatt atatttctga tgctttggct
    27241 gctcaggttg ggggaatcgg tatagctccc ggagcgaaca ttagtgatca aatggctgtg
    27301 tttgaagcaa ctcatggaac agcccctaaa tacgctgggc agaataaagt gaaccccggt
    27361 tctattattc tttctgccga aatgatgtta cgtcatatgg ggtggtatga agcggctgat
    27421 ttgattatca gaggtatgga aggcgcgata gaagccaaaa ccgtaactta tgattttgaa
    27481 cgtggtatgc aaggagcaac gttagtcagc agctctggtt ttgcagatgc tatgattaag
    27541 catatgtaaa ccggggagtt atcacatggt ctttctgaac aacgcgtatt gacaatatag
    27601 tgcttacagc gttactcagt gccaatgcgt tgttcatgaa agactgggat tatcctttca
    27661 agattattgg tagtatcttt gactatcagg tttatacatc ttacgagttc atgaaaattg
    27721 agcaggaact ttatccatgc taattatgat gctactctac tgattagaga cggctcaatc
    27781 tcttgagtaa aacctggtgg tcaataacaa atagtacaac atttgagtga atcatatcta
    27841 atcactatgc tattgattat ttcaatgact aaatttttca ttaaaattag ctaaatttac
    27901 tggccaaatg cgtgtaggga gtctataaaa atagtaagag gttgttagtt atgagcaggc
    27961 aaaatttaga ggaaataata caaacaggga tagctgatac agagcttagc acagaaatta
    28021 gcacggctat taaacggcca agaaagtata aggttttatt gctcaatgat gactatacgc
    28081 caatggattt tgtggtagag gtactgaagc acttttttca tttaaatgag gaggttgcga
    28141 ttcaggtgat gttacaggtt cattttcaag ggaagggggt ttgtggagtg tttacacgag
    28201 acatagcaga aacaaaagtc gccctggtaa atgaatacgc cagaatgaat caacatccat
    28261 tattatctag tatggaaccg gagtaaactg gtggtgggtt ggtaaggagc aatgacaatg
    28321 ttaaataaag aactagaatt caccttgaat ctagctttca aggaagcaaa agaaaagcgt
    28381 catgagttca tgactgttga acatttgctg ttgtctttgc ttgataatcc agcagcagga
    28441 aatgtattac aggcttgtga tgctaacatc gaggcattac gtcgtgattt aatcgaattt
    28501 attgatgaaa caaccccaag aattcctgag gatgaactgg acagggaaac tcaaccaaca
    28561 ttaggttttc aaagggtttt acagcgtgca gtatttcatg tgcagtcagc aggtaaaaca
    28621 gaagtcactg gagctaatgt attagccgca atttttagtg agcaagaaag tcaggctgta
    28681 tattttttgc gacgtgaaaa tattactcgc cttgatgtca ttaattatat ttctcatgga
    28741 gtgtcaaaat atcaaaacaa cgatatgcat gaaaacatga attcgtctat ggaagaagac
    28801 atgatgaccc ctgagggtaa tgaatctcca cttgatagtt attgcaccaa tctcaacaga
    28861 cgcgcaaaac agggcaagat agatccgttg atagggcggc atgaagagat acaaagaact
    28921 atacaagtgc tttgcagacg tcgtaaaaat aatcctttat tagttggcga ggcaggtgta
    28981 ggtaaaacgg ctatcgcaga aggtttggcc agacgcattg tggatggtga aatcccagat
    29041 tccataagag gctgcgtagt ctactctctt gatttgggcg ccttattggc aggaacgaaa
    29101 tacagagggg attttgaaaa acgattgaaa gctgtgctga aacagttggg gcagcaagaa
    29161 ggagccattt tattcataga tgaaattcac accattattg gcgcaggcgc tgcatctgga
    29221 ggagtcatgg atgcttccaa cctggttaag ccattattgg caaatggtga attgaaatgt
    29281 attggctcaa ccacttatca ggaatatcgt ggaatatttg aaaaagacag ggccttggca
    29341 agacgattcc aaaaaattga tatttcagaa ccaacaatcg atgaaacttt tgaaatatta
    29401 aaaggattaa aaggcaaatt ggaagctcac catggagtaa aattttcaat tgcttcatta
    29461 aaagcagcgg cagaattatc tgcaaaatac atcaatgatc ggtttttgcc tgacaaagcg
    29521 attgatgtgg tggatgaagc aggcgcttat cagaatttac tgacagcaaa taaacgtaaa
    29581 aaaattatta gcgtgactga aatagagagt gttgtcgcaa aaatcgctag aatcccaatt
    29641 aaaaaagtat ctgcacgcga taaagatact ctgcgtaacc tggagcgtga tttaaaatta
    29701 ttagtctatg ggcaagacca ggcaattact gccttggctt cagcaatcaa actgggtaga
    29761 tccggactta gagaacctca gaagccagta ggttgtttct tgtttgccgg accaactggt
    29821 gtaggaaaga cagaggtaac aaggcaactg gctaatgtgt taggcataga gttgctgcgt
    29881 tttgatatgt cagagtacat ggaaaaacac actgtttcac gtttgattgg agcacctcct
    29941 ggctatgtag gatacgatca aggtggttta ctgactgaag ccgtgactaa gaatccacat
    30001 gctgttctgc tacttgatga aatagaaaaa gcacatcctg atgtttttaa tttattgttg
    30061 cagataatgg atcatggcac tttaactgat actaacggtc gtcaagctga tttcagacat
    30121 atcatacttg taatgaccag taacgcagga gccagtgaaa ttgttcgtaa ctccataggt
    30181 ttttcaacgc aagacaatac caatgatggg ttggaagtaa ttaaaaagca attcagtcct
    30241 gagtttagga accgtttgga tgctattatc aattttgctc cgttagatac acagactata
    30301 ggactagtcg ttgataaatt cattatggag ttggatgaac aattaagtca taaaggcgtt
    30361 acttttacag ttgataagtc agccagggaa tggctgatgg agcatggtta tgataaagcc
    30421 atgggagcaa gacctatggc cagattgatt caggaaaaca tcaaaaagcc tcttgctgat
    30481 gaactgttgt ttggtaaatt aatgcatggc ggtcatgtca ctttgcgagt gaaagacggt
    30541 aaactgcatt ttgacagtca tgatcataga gaaggtgttt gttaaacggg aaccatcttc
    30601 agttctgtgc atgacaaatt ttttgttatg cacagacaaa atcacaataa tcctgagata
    30661 gtaattgtca ttaacttgtt agttgcataa ctggtaattg ttatcacgat ttctttttgt
    30721 gaccgctaaa cgaatattat tcctttttac cttgttacaa tccggcttaa ttatcgatat
    30781 aatatttatt ttgaattgat ataaagccat gttatccgta atcattatca caaaaaatga
    30841 agaagccaac ataaggcggt gcttggaatc cgttcatttt gcagatgaaa taattgtatt
    30901 ggactctggc agcacagata atacattagc tatagccagg gagtatacgg ataaagtatt
    30961 ttcaacagat tggcaaggct atggcatcca aaaggatagg gccttacaga aggctcaagg
    31021 ggattgggtt ttaaatcttg atgctgatga atcagtaagc tcggaattac gacaggaaat
    31081 gctacaggcc atttcatcgg atacagcaga tgcatttcgt attcctatac aaatgatttt
    31141 ttataatcaa gttttaaaat attcaggaag tcctaaaagg catatacgat tatttaaaag
    31201 agagaatgct tgttatagca aagatatcgt tcatgaaaaa gtattgattc ctgttaatgc
    31261 gagagtgggg aaactaaaaa aacctatttg gcatcattct tttagcgatg tacatcatgt
    31321 gctatacaaa cttaacaaat attcttctta tagtgcgaaa atccgtatag aatccaatga
    31381 aaaagttgga ttagtaaaaa ctttttttag tgccctatgg atgtttgtca gatgcctttt
    31441 tttacagaag ggatttttag acggcagggc aggttttttg tttgcagtat ttggtgcgca
    31501 aggcgctttt tatcgcgggg tcaaacaaat atataaagac agggatatca ataaattacc
    31561 cagtgtaaat catataaaag aggaataatc gtgcgattaa ccataatgga taccaagggt
    31621 attttattag ctcctttttt ctttatctca tttatgtttt ttattccgat cagtcctacg
    31681 ataaagtcta tttgtttcat ttgcagttta attgctgtat tgattactcc acaatacaga
    31741 aaacatattg tttatgctta taatactttg tggggacgcg ctgcattttg tttgtttctc
    31801 tttgtcgtta ttgcaagttt atggagtccc gctccttatt caatgcaatg gatggtcatt
    31861 ggtaaatatt gtaaattaat ttatcttccc attttagcta ctggttttat taatcccaaa
    31921 gtaagaaact ggagtatcaa tagctatttg gctgctattt ttattacttg tgttatttcc
    31981 atcttaaagg ctaaaaatat tattgcgtca gaagatccgg gtgaggtttt ctacaatcat
    32041 attattactg gttttatggt ggcattggca gcctatttaa caggattgct ggcttttcaa
    32101 aattcgggct ggttacgact tatttatatc ttcatgatgt ttctaaccag ttaccaagtg
    32161 ctttttataa atactggaag aacgggttat ttggtctact ttattttgat gttattgctt
    32221 ttgatacaaa aattaaactt caaacaggct ttagcaggaa ttgttctctt cagtggcatc
    32281 attgctttgg catatcatca tagccctgta atgcaaacca gaatttatga tttaattcag
    32341 gatatcaagt tattaaaaca acataataaa aatacatcga taggctatag aatgcagttt
    32401 catcactatg ccaagcaatt gatgttgaaa catcctttta ttggaatagg cacaggtgga
    32461 tttaaataca gttattcaca agacgtacct gtcccaagtt ggggtaaaga gttgacagat
    32521 ccacacagtc agtattggat gactttggct gagcaaggtt taattggtct ggtacttttg
    32581 gtaatatttt tagcgagttt gtttataaca tcatttcaat tgactagctc cagacctatt
    32641 cttttaggtg ttttattttc tttttgtata ggttctattt ccgacacgat tttatgctat
    32701 tcgactgcag gttatttgtt ggtagtgatg agtgccttat gttttggtga gttaattgag
    32761 aagcgtagag tcaagggcgt agaagaagat catcttgttc ctttatcagc ccatgctcca
    32821 gcagcaataa gtcagaccta atcaataaac agaaatttgc tcaacaggtt tttgcaatga
    32881 acattcttcc gaaacatacc cgtcattgca aaccacaaga cccgatgaat ttaattgtgg
    32941 atagaccccg ccatgccata agcaacatcc cataagaaat tgtaaatcca ttacggcatg
    33001 acgtgtgcag tagcaagttg aatagaaacc gttgttacaa acaagacgac cagcagaaga
    33061 atcgcaataa tttattcccc ccattttact gcaacaacca tcagcagcga aagcaggaga
    33121 tgctgacaaa gcaaacacac tggctagtaa aaatgctttt ggtttcaata tgttcattgt
    33181 ggttctctga ttggttttta tttttaagaa tagcatagag ttagatggat tattagtgaa
    33241 atatgatgat ttaattgcag tttaagactg aatttattat gacaaaaaag ttcaaagtga
    33301 aatgataaac tggtttcatg ggccagctat tgtgaagagt taatgattaa taaatgagaa
    33361 tctgaggata gacttctaat aaatagctgg tgagaaaacc tgctaatagc ccaaacaagt
    33421 ggccttgcca ggatacgcct ttttcacctg gaaaaattcc cagaaaaata ccagcaaaat
    33481 aatataggct aagcagacca agaataatag tggtcaaggt acctgtttga taaatgttat
    33541 acaccaggaa tccccagtag cctgtgatta aaccactggc gccaatgtgt atacctcgtt
    33601 tagcaaaaca ccaaatcagt gttccgctaa aaagagttat catcactgtg acaatcaaat
    33661 aatagttaac tccattaatc agaataaaat tacttaaaac cagtaaaggg atagagttaa
    33721 aaaacaggtg attaaaatta gcgtgaagta aaggtgctaa tagtatcccc gggaggccca
    33781 aaatatgcct tgggataatg ccaagaagca aaattttatt acttataaaa taactaataa
    33841 aaaatattga ccagggaatt agtagtatta ctcccaaaat tttcaggtta ttttgtgtct
    33901 gagtgacaat catattcaaa ctggccgtta attcgttaat catgatttta ccgttgcatc
    33961 ttgtgttgat ttgtttgatt ttttatctgg tataactggt gtaactgtga ttttgcgaaa
    34021 cggtacaaaa agtaaaggtt caaccaaagt ttggttaact accatagtcc agtggagatg
    34081 ggggcctgtt actcttcctg tcataccaac taatccaagc tcttggcctt gtttgactgt
    34141 ttctcctgtt ttaaccagaa ttttgctaag atgagcataa accgagaata ctcccatgcc
    34201 atgatcaagg ataaccgtat tgcctgtgaa aaaataatca ccggtatctg ctacaactcc
    34261 ctgatttaca gcatacacag gtttcccctc gttagcggcg atgtccaatc cagaatgcgg
    34321 atctcttggc tgtttattat aaactctctt caggccaaat aaactggtta taggtcctct
    34381 cagtggtgct gtgaattgtt tctcaaatgg attggtattg gagtaattag caaaaatttg
    34441 ggttaatttt ttggtttctt tttcaatccg gattaaatct tcgggttgag gatctacttt
    34501 actgatatct tttatattta aaaattgtgt actgtaaaat ttttcactaa catgaaaggg
    34561 aatagatgct ttgatgggtt ttgtcacgtt gatgtattga acagagttgg tatttttaag
    34621 cgggattgca acgattaata accattgtcg gggttgggta ctgggaagaa ccggtatgcg
    34681 tttcccctcg aaataggctt caggcttctg attgaaatca ataggaataa tagtcagtcc
    34741 cccattgaca gaatgatttt ctggtagagc caggccatag gatagttttg ctgagcttat
    34801 taatatcaat aggcaggcaa ctgaatgtaa tagtttcttc ataattttag tcaatcctta
    34861 atttgagtaa cctcacaagc aagacttcct tttgctaaac ggatattgat agtatctcca
    34921 atttgtgcct gttgggcagc aaagagtatt ctttggtttt tactcacaat agcataacct
    34981 ctatctagtg tagccaaggg gcttacagca tgcaaagtgg aaagattagt ggatagttga
    35041 ttttttaatt ggtttacttt tatccggatc tcttgggtta attgcattat taattgttgt
    35101 agttggattt tagttcgttc aatttgtatt tttggattgc tagcttgcaa ttgtgttgaa
    35161 aaaatattca ccttgttaat gtttaagtta ataaattgag tcatagcaga tattaactgt
    35221 ctctccagat aatccagagt ttgccagtat gttgagatag cttgcctggg tgaggcaatt
    35281 ttatctatca aatgattgag ctttaattgc aaccctttaa ttagtctgat gatggcatca
    35341 tgcaatcgat aaatagccgt atcaagaata ttgaataatt cgatgcagtt tggagtaaca
    35401 gccgtagcgg cagcagtagg cgtttccgca cgataatctg ctacaaaatc tgcaattgtg
    35461 aaatctgttt catggccaat accagaaaca acaggaattt cgctgattgc aatttgtcga
    35521 gcaaggtatt catcattaaa ggcccacaag tcttcaatgc tgccaccgcc gcgtgccagg
    35581 atgagaacct ggcatcgttt atgggcatta gccagtttta aggaattgac tagttgttga
    35641 ggggcggttt gcccttgaac ctcacttgga taaataataa ttctggctat tgggaatcga
    35701 cggtccagag tcgagagaat gtcctggata gctgctccgg taggtgatgt aatgatgcca
    35761 atcgtttctg gtattctggg cagtgttttt tttctctcag gattgaataa tccttcgctg
    35821 gccagtttga tttttaactc ttcaaatcgt tgatagagaa tccccattcc tgcttcaact
    35881 atctcttcga caataagctg atattctccc cgggcttcat acagacttaa tttgccagtg
    35941 gcaacaattt gttgaccatc attaaaattt ctaagcagag aattggaatg tcgattttta
    36001 aaaaaagcgc aacgaatttg cgctgtgctg tctttcaggg tgaaatagta atgtcctgaa
    36061 gaaggtttgc ttaagttaga gatttcgcct tcgacatgca caaggcctat ttcattctcg
    36121 agaaaacctt tcacttgtct attcagctga ctcacggtca gtattggcaa ttgactactc
    36181 atggtggtaa aaatcccatc aaatcaaaag gttgttaaca acaataacta attaaagaaa
    36241 taaaattata ttgtagtgag atgttatttc atttaaggtt atacgttact atatagataa
    36301 ttaacaaaat aacaataata aggaatgagg aatgagaaag gcgctgtttg tagttgcaat
    36361 gcttacttct tcctacacca ctgcagatac tctttatgaa gcagtacaat atggcatgat
    36421 cgctaacccg gatatactac ttaatactgc aaaaggtttg tcagcgaaac aggcaattga
    36481 taaagcaaag ggtggtttat atccttctat cgatgtaact ggtggttttg gacgacaaag
    36541 gagtgttaac ccgacatcag cagcgatcga tgatactccc agcactaccc tcaatatagt
    36601 ggaatcggcc gtggaattaa ggcagcgatt gtttgctggt ggagggataa ttaatgaagt
    36661 aaagcgaaac caatacctta ctgaggcgca aaagtggaaa acacagggta ttgctgaaga
    36721 tttggccttg gaaattacca aaaattattt cgctgtatta ctgcatgaac gtttgtatgc
    36781 ttactccatc gaaaatctta aagcacataa agcagttttt aagatgataa aagaacgggc
    36841 tgatgccggg attaccagga cggcagaagt cgatcaggct aatgcacgac tggctctcgc
    36901 tgaggctaat aaaataagtg cattggctga tttgcaagag gtgaagatca attatgcaaa
    36961 aacagtaggt aaatggccag agaatcttca aatgcctcat gtcccagcaa gaaaaagttt
    37021 acctaataat ttagccagga ttatagaaaa aggattggat aatcacccaa cagtaaaatc
    37081 aagttatgct gatatcaaag aagcaaaggc tcagtacgat gtcgccagag cagcctatta
    37141 ccccgaggtt aatctggttt tgaatagttc aaaaaacaag aacttaggtg gtttgatagg
    37201 tcctaatgac tccgacacag tggctgtaag aatgaattac aatgcatttc gtggtggtgc
    37261 cgatgccgct cgtatacgag aaacggctta tcaagttcag gaagcttatg aaacaaaaaa
    37321 tcgaacattg ctggaattaa aagagacaat caggttagcc tggaatgcct atgtagcctc
    37381 tgccttgcgc atccaaccct tgaagcaaca cgttacagct tcgagaaaaa cacgtactgc
    37441 ctatcaagat gaatttaagg tgggtaaaag aacgcttctt gatttactcg attctcaaaa
    37501 tgaatattat caatcacaaa ttgaattggc ccgcgcagaa aatgacgaga tactttcccg
    37561 ctaccgaatc ctgaatggaa tgggttgttt attaaaatat ttgaatttga ggttgccggt
    37621 aaatgtagtt aataatgatg tattctcgtc ggctcagacc aatattttat tgaacaggaa
    37681 aatggatgag atcccttatc ccaataatac ggataatcct atggttctca ctcatcctgt
    37741 taagaacatg gagaaaactc gtttaaccaa gtcgatggta gacaaaaaca ctacctatcc
    37801 tcagcaaata acacccaagt tatggtatat ctcagcaggc tctttcagag atagaaataa
    37861 tgctatagct ttggttaaac ggttaaatgg attaggcttt actgccttca tgagccctta
    37921 taaagatctt ctgtccgttt ttgttggtcc ttatgaatac agaggtcatg cgggtaatgg
    37981 aatggaaagg ctaaaggaat tggctcatgt tgaaggagtc ctggttactt tcaaagagcc
    38041 gcccaaaaag gcttagtgtt atagccaaga gattccagaa tgttcttttg gaatctcttc
    38101 gttatagatt ggttttatcc atttggactg gtttgatact ttgctttata acaagttttt
    38161 ccactcaagc cgcgccttta atcagtgtgg aaaaaattca aaaattggcg caatcctatc
    38221 agggggatac ccggaaacgg tttaccgcat gggcgaattt aattgaatcg cttaaaaaaa
    38281 agccagtaaa aatacaactg gaaaaagtaa actctttctt taatcaattt aattatgaaa
    38341 ctgacccaat aactggcgca agtgatgatt attggaaatc accggtagaa tttattgttg
    38401 atgggggagg tgattgtgaa gattttgcca taataaaata ctttacatta gtcgctgttg
    38461 gtgtgccttc tgaccagtta agaatcactt atgctgcttc tcttactcta aaccaggctc
    38521 atatggtgct gtctttttac ccaactcctg aatccgaacc actaatcctt gacagtttgg
    38581 aaagtaaaat tctgaaagcc tctgcaaggc ctgatttgaa accagtttat agctttaatg
    38641 cggaaggttt atggcttgcg aaaacaaggg gagagagttc attgatgggg gattcaaaaa
    38701 gtttgggcaa gtgggatgct ctaatgaaac gtatggaata aaaaaggtga tagaccatga
    38761 cattaactaa gaaaatggct gtaggcgtgt tgattatgtt gttattcatt tttatcggaa
    38821 cttatttcat cactatgaat aatgcgcgta atttttttat ccaacaactt gaaagcaatg
    38881 ctcaagatac tgcgacatca ttaggcttgt ctctatctca gtctttaatc aatcatgatg
    38941 tacctaccat ggattctatg gttaaagctg tttttgacag gggatatttt tcatcaatta
    39001 aggtaaaaga cataaaaggg aaagtgatca ttttaaagaa acaattgcca caagaaagcg
    39061 atataccaca atggtttgtg aacttgataa aatggccatc aactgagaag tcatccttaa
    39121 ttatggatgg gtggatgcaa gcaggagttg ttttagtcgc tagtgatccg agctatgttt
    39181 acgcctcttt gtggcgtaat gctgtagaaa tggtaaacgc ttatctgatt tttgcgttag
    39241 ttgctttggt tctaagctat tgctttataa aatatttatt gcaaccttta aaacgagtca
    39301 cagcacaggc tttggcaatt tcagaacatg agtttcctgt agaaacaaag attcctaaaa
    39361 cccctgaatt gaggcaagtt accttggcta tgaatcaaat ggttactaaa gtcaaatcct
    39421 tatttcaaga ccagttaaaa caaacagaat cacttagaac acaggtttac caagattcat
    39481 taacagggat gagtaatcgt cgttattttt tacagcattt ggctctgatc ctggataatg
    39541 aagatgaatt tatacctggt tatgtcatga tgctggtcat agatggtctg gatgagttaa
    39601 atcagaagca agggtatcag caaggggatc aattggtatt aactgtagcc aaaatatgta
    39661 aaagctattg gaagcaatcg tctgttagta cattggcaag aattaacggg acgacctttg
    39721 ccttgattag tcatgaaagg gatcccttgg tgtttgaaaa ggaatgtaag gagtttgaac
    39781 aaattttgag ccaaggtatc actgatatta aaatttgcga aacacacatg ggggctgcga
    39841 gttattttct ccaccaacct gtctccaatc tgttgtctat agtagatcaa gcggttaaaa
    39901 aagctcgtga aactggcgta ttttattgcc agaaggagca tgatacttat aaatatcccc
    39961 agttaatcag tggcgatgaa attagaaaat cattggaaca gaaaaaaata agtttgtatg
    40021 cgcaggcagt tacagatggt aagaattgtt ttcataaaga agtcttcgtt cgaattcgca
    40081 accaggaagg ggaggagtta ggcgctggtt attttatacc agttgctgag aaattagggt
    40141 tggcctatct tattgatcag tatgttctca atgaattaac cgtcatggac attgcaacgc
    40201 atacccattt tgctcttaat atttcagaag atactttaac aaataaagtc aatagcactg
    40261 gttatttgcg gcaattggaa gctactcctg cagctgtctt gagaaatctc tcactggaaa
    40321 tcaatgaagc tcacgttttg tcacattttt caaactccaa gttctttatc aagcaagcca
    40381 aaaaattggg ggtgacagtt ggaatagatc gtgtgggtat taaattttct ccgttgcatt
    40441 atttgagtga tttaaacata gattatttaa aactgcatgg aagtttggtg acagatattg
    40501 atgaaaacga aagcaagcag tttttcatac attattttaa tgaaatggct aaaacgatgg
    40561 atattgcggt ggtagcaact caagtggaaa gcgaggctca atggcaagca ttacaaatag
    40621 tccacatacc ttgggggcag ggacgctttt tatcttctgt agaattaata aaataacgag
    40681 atgaaaaaca gattattctc aatgcattaa aatgaattcg tgaatagccc taaacccgct
    40741 tggtgcctct gtaaaatggt ataagaataa ggtatgataa aaaaacaaat aattacaata
    40801 ataatattaa tatgaactat tttggcaatc tgcaaatacg tttttccaaa acatttttat
    40861 tgttgttgat tgttagtagt ttaacagctt gtgtggattt gacgggcgca cgaaatactt
    40921 atatcaagaa agaggtgcaa cctgcaatct ctgatgtccc taacaaaaag tccaaaccac
    40981 aaaatatgtc atcaaaaaaa ggcgaagtcc atactatgct tggcggcctg ggaattttca
    41041 gcaaggggat gtacacactt cgtgataaag tgtctgaaaa ataccatatc acagcccata
    41101 gcaccatgtg gtataacgct ggagcggtaa gccggtatat tattgatcat tactatcaag
    41161 agaaagatca caagccaatt attttagtgg gacattcttt aggggctaat gaacaaatca
    41221 aagtggctcg aaatttgaat gccgcaggtg tccctgttgc tttgttggtg actgtcgatg
    41281 cagtatcaca aactattgtt cctcctaatg ttaaacaagc tttgaatgta tataaacctg
    41341 gttttgtgcc tatgtttagc gggttaaaac tcagagcagt cgatcctcaa ttgacaaaga
    41401 ttgataatgt caatgttgat tcgataaaag ggataaaagt aaatcatttt acaattgaca
    41461 aagacgaaat tttacaagcg atgataatgg ataaagttga taaggtgtta gttgatggaa
    41521 ataaaaaggg tgcttaagga ccgtctgtct gctcgtgttt gtgttatcgg tgctggtccc
    41581 agcggtattg ccgccattaa aaatttgcaa gaacacggca ttactaatgt tactgttttt
    41641 gaaaaaaaca atcaaatagg tggtaattgg gtttacgatg agcaaaatga gcattccagc
    41701 gtatatgaga cgacccatat catcagttca aaacgctggt ctgagtttga agatttccct
    41761 atgcctgttg attacccgga ttatccttca cacgcccaac ttttaaaata ttttcagagt
    41821 tatgtggagc attttcattt agctcgatac attcgtttta acacagtagt acaaaaggtg
    41881 catcgacttg atgataatac atggcatgtt atctatgagg atgcccaagg aattcatgag
    41941 gcgtgctatg actatttgct ggtagcaaac ggtcatcatt gggatccctt tatgcctgta
    42001 tatcctggag tatttgacgg gcagatactt cattctcacc aatataaaaa agcttcaatt
    42061 tttaaaggca agcgagtatt ggttgtgggc gggggtaatt cagcctgtga tgtcgctgtt
    42121 gaaatatcgc gagttgcgcc tggaacttgc attagcatgc gtaggggtta tcatattttc
    42181 cccaaattta tttttggtaa gccaacggat gtagcggtag ctaaaataca atggatgcca
    42241 tcctggctaa gacagaagtt catcagcctg gtcattagag gattgcaagg ccggtacgcc
    42301 aagtataaat taatgaagcc tgattgtggg ccattggaaa ttcaccctac tattaattcc
    42361 gaactcttgt attttattcg ccatggcaaa atacaccctc gtcccggtat atcgagattt
    42421 gagggtaaca ccgttcattt tactgatgga acccaggggg agttcgatac gatcattttc
    42481 gctacgggtt accaaatcag ttttccattt tttgataaaa gttgtattga ttttagtaac
    42541 tctaccacca tccctttata tcgaaaaatg atgcaccccg agtttgaaaa tttgtatttc
    42601 attggcctat gccagcctca aggttgcatt tggcctttgg ctgattatca atcaaaaata
    42661 gtggctcgca ttattgcagg gtctttgaaa cgacctgatc aattacatcg aaaaatagcc
    42721 aaagaaatca ataaacccca tcaccgattt aaatctcaca tgcgccatgc tctagaggtc
    42781 gattatcata ttttcagaaa agaattgtta gaaatgctag gctaaaaaca taggagactg
    42841 aaattctacc cctagattcg taatgagtac atgaatgtta aagcatggat gatcacatca
    42901 gttataaacc gggtaacaat tattctgttt tgaaaatcgc aaacaattta cagattggct
    42961 tctgatgatt ggcgctttaa taaagaacac attaattgtt cgacgctgtt acgatgcgga
    43021 ttttcctgca acagtatggc tttggttctg tcttcacaat atcctttaac ttctttatca
    43081 ggcagtatgt aaaatctttt ttgttcaact tcccttacta tatgttctgc aacttcaatg
    43141 gcgggtcgag aatgtgctaa caagttattt aattgattgt ggattggaga atggtttaat
    43201 ttcccactac tcgctaataa tgaggtatct gtaaaagaag gaaatactac agaaacatct
    43261 acaggttttt taagacgatt taaatcaaaa tataatgact cggataaggc taatacagca
    43321 tgttttgaca tggaataagc ggccatttgt gagcctgtac ataaggcata caggcttgcc
    43381 atgttgataa tatgagaacg gaaattctgt ttgaataaat aaggcatgaa tgagcgaatc
    43441 acattaagca tcccgtgcaa attcacttgc attacctgat taatatcatt ttcttgcagt
    43501 tcccaggcag ggagtaaatt accaatgatc ccagcattat taaatatcca gtctacctgg
    43561 cctagttgtt tacaggaata ttccgcaagt cgttctactt ctgatggttt ggttacatcg
    43621 caggcataac aaataacatt ttcaggagaa aatttagtca tgaatgattg ggcttctgta
    43681 tgtaatttgt cttgattctt atcaaccatg actatagttt ttccattgct aagacaaact
    43741 tgacttaagg catgtccaat gccacttgct gcaccagtga taattactac attcatataa
    43801 aataccttac aaaagtttaa tgccctcttc ttcaggactt acgaaaaacc ccaatctctt
    43861 cgttaaaaaa agtttttaat tcggtcattt agtttatcta aactccctca ttaaaaactt
    43921 tttttgcctt ggccttgggg tttttcgtaa gtcctgtttc tgtggcgcta gggaggcatt
    43981 gaggtttcta atgtggattt tatgaaaaag tgtatcatgg gcaagagtat ttaccaaaat
    44041 gattgctaaa attgattttg ctatactttg aatagaacta tcagaaggag gcgatacttt
    44101 gaatacaact gaaataccag aacagccaat tgagtatcca gaacagccaa ttgagtatcc
    44161 agaggagcca tcagagtacc ctgaacaacc tttggagtac cctgataatc cagagcctct
    44221 ggagcctggt tatcccgaat tacctgagcc tgagccaagt caaactcctg agccaggttt
    44281 tgagtagttt atttagtcag tcattagttt ctgtaatgct ttcccgatgt tatctgccct
    44341 cattaaactc tctccaatca aaaaggcatt aatgccatgg gattgcatta attttatgtc
    44401 ttcacgggtg ttgattccgc tttcagtaat aataatttta tctttaggaa caaattgttt
    44461 taattggata ctcagttgaa tgtctgtttt aaagttgtgt aggctgcggt tattaatgcc
    44521 tataagaggg gtgggtaagc gtaatgctct ttctaattca tcttgcgtgt ggctttcaac
    44581 caaaacagac attttaagtt cttgagccag ttggcaaaaa tccattaatt gaacatcatc
    44641 taataatgca acaatcagta aaatgcaatc agcgcccaag acaaggcttt catagatttg
    44701 ataagaatca ataataaagt ctttacgcaa tacgggcaaa gtagtttttg attttgctaa
    44761 agccaaataa tcaggatgac cttgaaaaaa ctcaatatcc gttaaaacag ataagcatct
    44821 tgcaccattt tgggtataaa tcttggcaat ttctgcaaca ttaaaatctt ttcgaattaa
    44881 acctttgctt ggagaagcct tttttatctc agctatgact gcaggtgagg cattggattt
    44941 taaagcagtt atgaagtcgc gtatttcacc tggttgttgt tttgacagaa catgtaaagg
    45001 cttatttttt ttcgcaactg caacttcttc tagtttatgt ttggctatgc gttccaggat
    45061 actattcatg atttgattct ttatttaaag tttgagtcaa taatcggagt ttgttaaaac
    45121 aatgatttgc cttaccacta tcaatggcaa tttttgcttc ctctatggcc gcatcaaacg
    45181 atatgccatc tttggcacaa taaatggctg cggctgaatt gagaaggact atatctcgag
    45241 ctggaccaga atcacctgaa agtacggatt gaatcaaatg caggctttgc tctggtgagt
    45301 cgactatgat tgcatctaaa gaagaatgct ttagaccata atcttcagga gaaatgaacc
    45361 attgcttaaa atttccatct cggtattcaa ctacttcgct tttcgctgca atactaatct
    45421 catccagtcc atcttgagag cttattacta atgatctttc actacctaag ttggccagga
    45481 cagtagctat tgttttgagc caattcgttg aaaatacacc aacgacttgt cgttttacct
    45541 gagccgggtt aatcagtggg ccaagtaaat taaataaagt tcttattcct aattgttgcc
    45601 ttgcagctct cgcatgctgc atggcggggt gataatgtgg cgcaaacaag aaagccaatt
    45661 gacattggtt gatgcagttt tgtacctgtg agtcagaaag attaagtata aatccggctt
    45721 gttctaatag atcagcgcta ccgctacgac tcgaaacaga acggttacca tgctttgcaa
    45781 cttttattcc tgctgcagct acaacaaaac tacaggctgt tgatacgtta aaagtatttc
    45841 ttccatcacc gccagtgcca actatatcaa tcaggggatt tcccaaatct attttgtggg
    45901 ctaattgcct catgacttta gctgctgcag taagttcatt gactgtttca cctttcatgc
    45961 gcattaatgc cagaaaggtt gcaatttgca catcactaaa ttcacctgtc atacaagcat
    46021 ggatgacctc ttgcatctgg tctgagctta aatcctgtcg ggatagaagt tgttcaaata
    46081 gaagtttggg tttcatagcg taaaaaattc tcgagtattt gcaagccaaa ttgacttaat
    46141 atggcttcag gatgaaattg tagaccaaaa agaggaaatt gacgatgtga tattgccatt
    46201 attgtatcgt ctgcccacgc atctatcgaa aagcaattgg gcaagctcat gatatccacg
    46261 gctaaagaat gatatctcgt cgcttgaaag tgatttggta ttcctgtaaa taagccctgg
    46321 taatgatggg tgatttctga ggttttacca tgcatgattt ctggtgctgg gatgattttg
    46381 cctccaaatg cctgagctat acactgatgg ccaaggcaaa tacccaatat cggaatatgc
    46441 ttgtgaaact ctttaatgac ttctatagag attcctgatt catcaggaga gttgggtccc
    46501 ggtgaaatga ccaggtattg cggcgctgat tgcctgatag catcgatttg aatttcgtca
    46561 tttttaaaaa ctgcaacttt ttgatttaag ctctggaagt actgtaccag gttgtatgta
    46621 aatgaatcgt agttatcaat aattagtaac ataagtataa ttaaaatgta ttcttttaaa
    46681 tgaggctaca aagcaaattc ttcacccaaa taaacttgcc ttacttgttg attggcaagt
    46741 attgctttgg gggttccttc gcaaagaatt tttccctgac ttacaatata agcgcgttcg
    46801 caaatgtcta aagtctctct cacgttatga tctgtaatta aaacccctat acctttatca
    46861 cacaaatgca ggataatttt tttgatatcg attacagaaa ttggatcaac cccggcaaaa
    46921 ggctcatcaa gcaagataaa ggatggttct attgccaggg ctcttgcaat ctcaacgcgt
    46981 cttctttcgc cgcccgataa ggccatgccc aagctgtttt ctatgtgaga aatatgaaac
    47041 tcttgtaaga gaagatccag cttttcttca cgtttttgat catctaaatc ttttcttagt
    47101 tccagaatcg ccatgatatt ttcagcgaca gtaagctttc taaaaacgga ggcttcttgc
    47161 ggaaggtaac taataccatg ccttgctctt aggtgcattg cgtcttgagt tatgtctgtt
    47221 ccgttaagaa taatatggcc ttcatcacag gattgcagac ctacaatcat ataaaaacaa
    47281 gtggttttac ccgccccgtt aggacctaat aaaccgacac attcaccttg cttaatgtga
    47341 atgctaacac cattcacaac cgtccggccc ttaaatgatt tttttaattg tttagcctct
    47401 agtaaactca tgatttcttt tcaggataaa taatgattgt cgttcttgtt ttttcgtcac
    47461 tatgtgataa aacatgttgt tcctgagtat cataactaat ttttgccgct gaaagagagt
    47521 tgggtccttg ctctatgcga gcattcccaa ttaattcgat taaatgtttt agcggataat
    47581 attttatagt gtcagcatag gcatggaatg ggggtttatc aggggctgtt gtagtccaat
    47641 aatgagcttg cttgtcttta gagccttcag caatggctaa agtgagttga tttttggcat
    47701 ctccttgcgt aatggctttt tctgcacgca aattggttga cccttgaacg aactcaacat
    47761 ttcctatata gactccctga tgatttttct gatttaagtc ggctgaatca gaaactactt
    47821 gcaccatttt ttccttatca aacggaagag catttcctga aatggagcac aacatcaaca
    47881 atagcagtat aatgtattta acctttagct gggtcataac tacctctggc tttatggagt
    47941 aattctactc ttttttcatc caaataggca ttcattccag tagactgtat gatattgcct
    48001 ggttgttcaa aggttacaaa aagacttgtt gttgcttttt tttccttggg ataataagta
    48061 atctcttcag tttttaaagt gctttccctg gtgttttctc ccggattttg atgaactact
    48121 acttgctcta taaaagtaat ttgtttacca ccatcaaacg attttgcccg taatgaattg
    48181 atttcccacg cgggttgatt atcttgctta ataataatat gggggttttt aagcaaatgg
    48241 agattgttgt taggaatata ttctacctgt ggagtggtta ataagttaat taacacacct
    48301 tctgaattaa attgctttac ggtaagctgc gaaatcgttg tgtcaacttc atttgacaac
    48361 gttttcttat tgataatagc ctgattaaaa tgagcataat accaaccaga tcctgctaaa
    48421 ataagcaagg tcaagaatag ccaaattgct tgcttggtaa tattcattgt tttaaatagc
    48481 ctgtgatcgc caattctgct ttattttgtg cgttaagaat taagtcacat aattcgcgaa
    48541 ccgcgcctct accaccagtc cgctctgttc tccaatcagc aaattccaga acttgcggta
    48601 cagcattact gactgcaact cctaatccca cttgttgtat caagggtaaa tctggcaaat
    48661 catcgccaat ataggcaaac tcctcatcat tgagtcccaa tgtttttttt agatgttggt
    48721 aagcgctacg cttatcaact tgacctttat agtaatgagt tatccctaat tgctccattc
    48781 gatggtcaac tacagcattt tgtgctgtgg taatgattgc aacttgtatc ccggcggcca
    48841 ttaataattt taatcccatt ccatcttgta catggaatga tttcagctca ttgccgtgat
    48901 tatcaatgtg aagtaagcca tcactgagca ccccgtctac atcacaaata agacatttaa
    48961 tttttttcgc tttttccaat agttcgttca tttctatctc tgtattaaaa aacacccgct
    49021 tttagcaagt catgtaaatg gagtaccgca taaggtctat tatcattttc aactactacc
    49081 aaggatgtga tgctatattt ttgcatgatt gctaaagctt cggcagccaa catgccttta
    49141 gaaatagttc tgcagttttt agtcatgacg tccttgatta gagtagtgtt gatattaaat
    49201 tgacgtgtca atgttcgacg gatatctcca tcagtatata cacccactaa atagccatgg
    49261 ttgtctacaa cacaagtcat tcccagcttt ttatttgtga cttcaattaa agcgtcactc
    49321 actgttgcgt tttcattcgc taagggaagt tgctctcctg tatgacataa atcgtctatt
    49381 tttagcaaca attttttgcc aagtgcgcct ccaggatggg aaagagcgaa gtcctctgca
    49441 ctaaatcccc gtgcttgtaa aagagatata gccaatgcat cccccataac cagcgatacc
    49501 gtagtactgg ttgttggagc aagacccaga gggcaagcct cttgtttaat actgacatcc
    49561 agattgacat ctgcagattt agccagagtt gattgtttat tgccggtcaa tgtaattaag
    49621 gggacttcaa gcctttttaa taatggaagc aaagtaacta tttcgtgggt attgcctgaa
    49681 ttggaaatgg caacaactat atcctggcga gttatcatac ccaagtctcc atgacttgct
    49741 tctccaggat gcataaaaaa agcggggctg ccagtgctgg aaaaagtaga ggccagttta
    49801 ttagctatat ggccagattt tcccatgcct gtgacgacga tacgcccttt gcatgataag
    49861 agtaattcac aagctttttc aaaatgttca tcaattcgtt gagttagctc aaatacagct
    49921 tgtgcttcag tttcaataac tgctagtcca agtgtgcaaa agttcatagg tcattgagtc
    49981 gtaattttgt ttggttttca ttctaacata atgtatttaa atgatgtacc ctatatttca
    50041 gtaagtgata ccgactaa
//
LOCUS       DBNCWK010000009        49052 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.009, whole genome shotgun sequence.
ACCESSION   DBNCWK010000009 DBNCWK010000000
VERSION     DBNCWK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 49052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49052
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.009"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            231..932
                     /locus_tag="AC06IO_000433"
     CDS             231..932
                     /locus_tag="AC06IO_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9873649.1"
                     /translation="MQKIKRSIILILQATDRGFKLKTLVITGISRGIGLEITRTFLDK
                     DWTVIGTSTSGYSPIKHKNLRTYKLDLAISEHIDAFAKNLPKIDVLINNAAVLLEEWN
                     EEKINMSLLKKTFAINLFGTVELTEKCISKFNDGAQIINISSGWGAFSSNNSPFQPHY
                     KMSKVSLNMYTKLLAERLPQITVSSFDPGWVKTNMGTQNAKKLPSETALEIYELISMK
                     KKSGYFWFNGKPRDW"
     gene            990..1523
                     /locus_tag="AC06IO_000434"
     CDS             990..1523
                     /locus_tag="AC06IO_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873650.1"
                     /translation="MSFDFIKSIWIAALIGITPTLSYSESNIQYQETLQSHCFETWMK
                     KIDPSIDKETYKQFGDKYCRCISTKNLDNKTGVQKAIRNCIPQTILHDAMDELEEEVT
                     LSEVTTQTIEQYCLNRWSLIYPNQSDEDKKTIQAYCACVKTKLLSFIEQIEKISNREY
                     NEAINDINTICSENLNQ"
     gene            1610..2533
                     /locus_tag="AC06IO_000435"
     CDS             1610..2533
                     /locus_tag="AC06IO_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9873651.1"
                     /translation="MSSKNSHSTLLNKQEINWILEQRITRLTDNKEVLIYPMGAERYL
                     SSALSGHEEERYNLHSEIKEKNVLIIPGYGNSAFLFAQAGAKSILVYDKDPVTIAWVK
                     AFKKYYHYREYNNNGKPYPSIGELLTALTKWYPPLLKLPSGVIKNSVFWIINPKLLRK
                     SYIYYMLKLTRQAVQLRVQNEFEFDHHIEFYAGELKHLIKNHPSKTFDTVFVPYLLGV
                     QNGIEKEKDIVSFIKQLIELVPYGHILVTPSKNTKEFHLVGQSYFCTTAYSNIQSIPD
                     LKPYVIAEDSIWFKTQGLAVFGIKAGAENHL"
     gene            complement(2667..5348)
                     /gene="pepN"
                     /locus_tag="AC06IO_000436"
     CDS             complement(2667..5348)
                     /gene="pepN"
                     /locus_tag="AC06IO_000436"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIO9873652.1"
                     /translation="MKIDQSKVKKLNDYKVLDYFVIHVDLQVDLSKKPVESKARLTVV
                     PNPNIDSHSNDLILDGENMTLVSLQMNDNLLKENEYELNKDALIIKNIPQNTPFTIET
                     TSLLGENTDLFGLYETEGVVLVKAESEGLRRVLYLPDRPDNLATYKTTIIANQQDYPV
                     LLSNGVLIEKKELPLGLHSATWLDDVPKPSYLFALVAGNLQRSVTYYQTKSGRELPIE
                     FYVPPSATSKCDFAKEVLKEAMAWDERTFNLECALRQHMVAGVDKYASGASEPTGLNL
                     FNTENLFATPETKTDLGILRVLEVVAHEFFHYWSGDRVTIRDWFNLPLKEGLTTFRAA
                     MFREELFGTDLIRLLDGKNLDERAPRQSAYTAVRSLYTAAAYEKSADIFRMMMLFVGK
                     ESFFQGVAKFFKDNDGGAVTLEDFIDSLSHSSDKDLYSFLSWFTESGIPELIVTDEFN
                     PDTKQYLLKIKTVNGKNRPIPVLMGLLDSTGKEIVADKLLILDQEEIEFQFENIQTRP
                     IPSLLRSFSAPVHMKYEYSYQDLLLLMQFDTNLYNRCEAAKQLISALLNDFCKGKKIE
                     LSPQFFAVYKALLSDNSLNEWMLAELITLPSLEELIENQDKPDFEKLNEGRQLIQNAL
                     ANELKTDFYNLLFRIQISGDDDKQKLKGFDLKQAGLRRLKSVCFSYLLNVDFEKTKEK
                     LILQFEDALGKNMTETALALSMLCEINCEEADVALEDYYHYWKNDPGAVNNWFSIQAL
                     AHSPDVIERVKKLMRHGDFDLSNPNKVYALLGSFIKNPFGFHSITGEGYLLVADAILD
                     LDKINPTLAANLTEKFTYWDKYDVNRQAMMISTLKIIYSNATSSDVRTMAKKGLDKVK
                     EDLPLPIHLTFHGGSTMQDRTTQLIADGNKENTYQLH"
     gene            complement(5568..7376)
                     /gene="lem10"
                     /locus_tag="AC06IO_000437"
     CDS             complement(5568..7376)
                     /gene="lem10"
                     /locus_tag="AC06IO_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem10"
                     /protein_id="HIO9873653.1"
                     /translation="MVTKIIWVSNNGKPNLKIEFISEEEKSNFFKEVKKKASELGLDF
                     PLVQGPGNSLVMQASNYPINPCGCYISPGGKLAVNFGKVELSHFILPKVGVKTEHAEI
                     FKDHNTIFFHKHKLPGVNSELTFIPTGAPVIVPVTKPATTSPAFFNPPKSGDSSISIS
                     AIGNVDSPMIRITFQNQTEREFFLNKITDKAKSLGVNISTHPFEIKEPNMVLIKPSKY
                     PDNKLGCYISKNKEIAINFGRTEFRDFVLSNLGVSSHLGTCPTKNETGGDTFYFHQEN
                     LSLNGPSLLVNTKITKEIEKNDYLLSANGSKAIDFVWSNFLKHPYNPKLKQPDANVKA
                     AWEKHADWEHWSNMGQDWSLNTPSGLVPRPNHGIAHTLRVAQLVPVIAEFLKAYSGDP
                     KFQKLTQKEIQKAQYMMLFSVIGRENDMSWTDANHYQQAFKEAYNGQHSKHIYATFKE
                     NAQKGFLNHVVTNKSSLIPSLFSDESELQYWAEALDTGKPGISSASGILMALAHDLDL
                     MRCYDKGKFNSLKMKDLVTRLGGNEEAARKLADYAHDLIVATGDRCMGYGMSQDYNFS
                     LFGQCSLDPNECLKKLQSVPKPETTLTTSLTNHHGI"
     gene            7677..8888
                     /locus_tag="AC06IO_000438"
     CDS             7677..8888
                     /locus_tag="AC06IO_000438"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIO9873654.1"
                     /translation="MHLIELLHKTFEEKLPQIHKSRLNSLLTACDAAIISNKLYLTGL
                     GRSLSNTNKECSNIQKVDRLLGNGHLQSERGFFYKLMTSHVIQEYTCPWIHIDWTCIN
                     PVTNLYGLRASVSMKGRSLVIYETCYPKKQENNHATHKDFLNQLKALLPVSVKPIIVT
                     DAGFRGLWFNYILKLGWDFVGRLRNKNAVCMGDTSTWCLSSSYFQLATSKPTYIGHGL
                     LTEKGKVPAHFVVYKGAPKGRKTLTLNTKKQRACKKNKVHSKGHKEPWVLITSLKKAS
                     LSPSLIVNIYRQRMRIEENIRDTKCPHYGLGLKDSLTQSPQRMNILLLIGAIATFAAW
                     LAGLFVKSIGKAADFQAQSAKFTSALSYVFLGRRALKKGLDIRKEEFKNTLLMLYHCA
                     LQAQQENPHYG"
     gene            complement(8952..10952)
                     /gene="ceg14"
                     /locus_tag="AC06IO_000439"
     CDS             complement(8952..10952)
                     /gene="ceg14"
                     /locus_tag="AC06IO_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg14/sidL"
                     /protein_id="HIO9873655.1"
                     /translation="MQNLDEILKKLGEDLKKTSKETANKLSVLSRKVAEDVVRVSDET
                     VQKINEVTKKFSVDTTVSTIDTQKILDDFMKNIRFSRFKQIWQAIEETPKSKHILLKS
                     LFLKGLLTHNKPLLEEIIRQIELIPYSSDLEMLGAFSQDKAHPLSPELLEFVQEMLAN
                     ESEKVLLTILMNAFQSIPELSLDSKTGTLSMKTKKELLPLLIEKTDTSIAKSIMSQLT
                     DISFDSVLPAFRPSIGDPSYQKTNINLSKYLSLVGDKTNLSEFLILTIGLFTSLKIGD
                     KEFLQELSADYLFVRYDDCLHKIIEKLKEKKVIEQLGQREVQKIMEASTNLKPFDPKK
                     NDPRRFFETRLAQYINGLSHSEKTIEIDSIGEDKEPEDEELRDNTLKACNKILQFFLG
                     DCGRVDTPREMEQKICIFANGYKDTSKFNKKEVEFITGVLHHAGAHKGRDRVERDKST
                     GIKERKFETDSEKVGSDVWNCGGGVMRGCSPVFYDAQREPMRNMLVDSSTVAPDNKEG
                     KQWFWNNNRQYSSYVGSISGHTCNIVGMLAKYMENYKEDPDLQNDINLFLIQVIGVYA
                     KRGFHAMLEVIDVLHDPYVQDIFKGYGVQVNLYSYFKENPELAGFLQHAMNDATAYTL
                     ALVNKEHVKVALLSHSLFSKKVEESGDKKNDHTPKDGNVYGC"
     gene            11214..12023
                     /gene="ankJ"
                     /locus_tag="AC06IO_000440"
     CDS             11214..12023
                     /gene="ankJ"
                     /locus_tag="AC06IO_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkJ/LegA11"
                     /protein_id="HIO9873656.1"
                     /translation="MITMGRSEMKIASAELRELMKAVSEGHYETVNTILDKDPELVNQ
                     YVPPTYDSPLARVLNKKHIDYKMLDILVKHHVDFDYPINHHKETPIELACHNQDLQLF
                     KYLVQHNAPISEHAPHFLLVNSTNIKYLTEDKIKNTCEIIKLMGGLEAVSSKCNAEGN
                     RFEELARKSQLINRFGGIVRYDYMQLLRSVYPIVDREVDVPTIHASTEKLTDLLNKIR
                     DQHTSKEGYDQQNLKDSISLFFMTGGEIPPSRKVPESCFEEAGIDAPKNTL"
     gene            complement(12178..12789)
                     /locus_tag="AC06IO_000441"
     CDS             complement(12178..12789)
                     /locus_tag="AC06IO_000441"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9873657.1"
                     /translation="MNKRNWTAYYNSTKQKTLPRKSLLKAIANFDKEKINLSKSAIDL
                     GCGAGIDVMELLRCGWSVIAIDSQLSAIDSLLSSAQELTCANKLKTIVSSFEALDSLP
                     KVNLINANFSLPFLKPVYFYKFWNVILKGLKPGGRFSGSFFGFNDFWNTRTDMTFLNR
                     EMICHLFSQFKIEYFQEEEKDEMDALGYYKHWHKFFIVAKKIA"
     gene            13051..13482
                     /locus_tag="AC06IO_000442"
     CDS             13051..13482
                     /locus_tag="AC06IO_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich domain-containing protein"
                     /protein_id="HIO9873658.1"
                     /translation="MHPDLKDLLDYRNHKIITRYNMDYPTAAMQAEEALHELMKFIWL
                     CHKHKTDKLLYPDDKNLDFSCVIHAEMADIDKMWHTFLLFTRDYHEFCDTCLNGNFFH
                     HDPLIGENTPVSEEDYALELTRYLTYIHDHLGSETLIKWFQ"
     gene            complement(13484..13864)
                     /locus_tag="AC06IO_000443"
     CDS             complement(13484..13864)
                     /locus_tag="AC06IO_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIO9873659.1"
                     /translation="MSHPDYSVYNEKCPSQNVLEGISDKWSILVISLLSQKIYRFGEL
                     KREIGGISPKMLTQTLLKLERYGFVQRQSYPVLPMKVEYSLTRLGKELSAILVLLTRW
                     TETNMDKIINAEKTFCEQKLGCHA"
     gene            complement(13886..14773)
                     /locus_tag="AC06IO_000444"
     CDS             complement(13886..14773)
                     /locus_tag="AC06IO_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIO9873660.1"
                     /translation="MHDTSALKKKLDYARFIHLVIMFMVAMLIFLFSTFPEKWWVLIT
                     VLSVSAGIEPGLIIRRAKHRIGGTLLALIILIPLLYLLQLNYRLISILFVLAIVGLSV
                     ATLNTRRYDISVFFITLVVFFLMAQTEEATSPRGPFEMVLNRGICTLFGVFIVLAGDY
                     FLFQAYRYSHRLYFFHQVIVYDFLNDIVRKIDESNTEKINTLLFVEKLRGQFTEHYLP
                     ISISSENLKLDFKTSEHTKKRIDIFQETIWEIRRLVFALCISEFVLHSSTASEQHLQR
                     FKLLMNKARTHFIQFEGRY"
     gene            complement(14752..15684)
                     /locus_tag="AC06IO_000445"
     CDS             complement(14752..15684)
                     /locus_tag="AC06IO_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873661.1"
                     /translation="MSMSKFIEWIDEVDPYAVQRIALYKSLFIATVMAYVYWVFRPTN
                     FTAFFSPFLLVRFYESPSLTSFKEKEHFLIFIGVVVVLLSTSFYLVYPFRVVFFFFSI
                     ITLASVYFYVLKNYLPLRNVTMLIIASGATILSTEPPANWQIVYDIVGSSALSMIAIF
                     ICLRFFPNQYLGVWKRALAKFIQSLELDIEGSFTHRGPKFMQEEMTHLGMLRQYRRLI
                     PKKYMIYTQRISINIRNIQFSLDNLYYEAKNEAFWHGVKENLYTLRSAIKTNTSCGTP
                     KMSIMPESKLQQYVMRCLQQAFSQWNQLCMILQH"
     gene            complement(15662..16699)
                     /locus_tag="AC06IO_000446"
     CDS             complement(15662..16699)
                     /locus_tag="AC06IO_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIO9873662.1"
                     /translation="MKKLMEYGGKIKEYLLRLKPKRWFTLTNVIIFIGFLTAIIYVFS
                     YLIPFTDNAFVVNNVRPVAALTSGYITELYVKNGDAVRKGQKLFTVFKKPYEYTVEQL
                     NADLAGAQAKLAVLKATYERDLKLSENEQKIYKKLAQDDQKYLKGYAIKSVSLITLQN
                     SQQETKAAKDKWQASLKQLEIDQHQITVQENEIKSIQARLKNAKVNLDLTDVYAQGNG
                     VIQNLFFTIGTPVNINQPLFSLVDQDNIYIQANFNETDLRDVRKGSKVLIFPRMYLGR
                     KIFHGVIDSDYWSANRQLVDDRTQLQNVINENQWILLPQRLPVIIKVTDPDPKYPLRV
                     GASAYVYVEVY"
     gene            complement(16838..18190)
                     /locus_tag="AC06IO_000447"
     CDS             complement(16838..18190)
                     /locus_tag="AC06IO_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIO9873663.1"
                     /translation="MMCMLKKLMFLLLLGAGLLSCGQSTDQQISNIITPKQFPSSPRD
                     YRPVQELPYLAWWKQFQDPELDRLIDTGLKTNMDIHIAMANLQQAQGELQQVKLSWIP
                     NVQILGGYSTNPALGVPGGFYGIWPSYILNIMQLYTQQKRAQYQVQYHQAAIDGLRLT
                     LIGQVAAAYFTLIAQLEQLKLLYKLDRDLKSLIALSRKEINIGLKNDIDLAQLQSNER
                     LIAAQIKPIKHNIVVSQNALRYLINENPGLIKSKNNFAQLDFTRFKPGSLPATVLGNR
                     PDMKMAEYSLKAARAGIFVAYSEFFPVMQLDDFIGEAHLPDSTFEQATDAYVNWTINP
                     NTLGKISVSKGVYNAKLAEYVKTVRLILKEVDNAFSANKRMREQFLSYRHAQEDYQHK
                     YKLQNGLLKSGLMSYKELLESKVYLDTLALSTNQAKLELAMSLVMLYQDLAGGYACSD
                     "
     gene            18277..18636
                     /locus_tag="AC06IO_000448"
     CDS             18277..18636
                     /locus_tag="AC06IO_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIO9873664.1"
                     /translation="MIKKIAFTMYPAQNMDRARQFYEKTLGLKVGKISAEGAWVEYDL
                     PQGGCFAITTLAQGVIPSATSGGSVAFEVDNLDELVFQLKSKGVQFKIDLFASPVCRM
                     AIAVDSEGNAFTLHQLT"
     gene            complement(18850..19281)
                     /locus_tag="AC06IO_000449"
     CDS             complement(18850..19281)
                     /locus_tag="AC06IO_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HIO9873665.1"
                     /translation="MFCIDDQIKSSSFILGEWPLSTVLLKNDASYPWFILVPRKMDIQ
                     EIYQLEKSERFLLIEEINQLSLLVSDYFKPDKLNIGALGNIVPQLHIHIVIRRKNDPL
                     WPQGIWQSSMPSIPYPESELKSLLPTLGDLVRAYDKPQSLC"
     gene            19518..19751
                     /locus_tag="AC06IO_000450"
     CDS             19518..19751
                     /locus_tag="AC06IO_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIO9873666.1"
                     /translation="MARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLVP
                     GQAVTYDVVNGERGLHASNVVALSENAEQEEIA"
     gene            19862..20857
                     /gene="hemH"
                     /locus_tag="AC06IO_000451"
     CDS             19862..20857
                     /gene="hemH"
                     /locus_tag="AC06IO_000451"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIO9873667.1"
                     /translation="MRRGLLLLNLGTPDNADIRAVKLYLREFLTDKRVIDLPTIPRYI
                     LVYCLILPFRSPKSAQAYQSIWTEKGSPLLYHSQNLVTKLQSALKDEYKIALGMRYGT
                     PSITTALAELKDCHSLTILPLFPQYSSAATGSAIEKTLSYLANQEIIPSIKIIRDFYQ
                     RPEYIQAQAKIMKPYIKDNFHVLFSYHGIPERHIHKSGCDTLCPQTCTPIYDKNQACY
                     RAQCYQTSLLLAKELQLGTHQYTTAFQSRLGKTPWIKPYTDEIFAELISKGIKNIVVS
                     CPSFVADCLETLEEIGIRAKEQWEKLGGEQFILTPCMNDHPEWIKAIQSIVNEQF"
     gene            20985..21524
                     /locus_tag="AC06IO_000452"
     CDS             20985..21524
                     /locus_tag="AC06IO_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HIO9873668.1"
                     /translation="MKKIKSVSHLLFLLFKTLAYGLPLVTTYFILFHFESMLNWGGWH
                     SLLSTQVKDPSHFSLLHRFIILLIEALPLSINVLICHQLAKLFRLYEHGYLFEEENIR
                     LIKNISIYLISGELIQLLYQPLITAALTFTNPSGQRIASITLGTANLSTLITAFIILI
                     ASWIIKEAHKLKTDALLTI"
     gene            21611..21847
                     /locus_tag="AC06IO_000453"
     CDS             21611..21847
                     /locus_tag="AC06IO_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9873669.1"
                     /translation="MSIIVNLDVMMAKRKCRLRDLAEAIGITEANLSILKNGKAKAIR
                     FATLEAICAYLHCQPGDLLEYQSTEDNHLIKDRA"
     gene            21973..23322
                     /gene="legY"
                     /locus_tag="AC06IO_000454"
     CDS             21973..23322
                     /gene="legY"
                     /locus_tag="AC06IO_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegY"
                     /protein_id="HIO9873670.1"
                     /translation="MATFFFKTNYYQEIMLKRIFFLMIFFVSQTMASVFTHEEVQILK
                     KHFLNNFQTNGAIVASPSQYNPNYYYDWIRDSAIAMGLVETWYEASQSARYKKLLLEY
                     VSWTEKIQHQADPIAGQDILGEPKFYINGNPFDGEWGRPQNDGPALRASVLIRFAQRL
                     LEQNEIDYVKSHLYNNTMDPQSMGTIKMDLEYIAHHWQDANFDLWEEVYGHHFFTAMA
                     QQKALTDGAILAHQLHDRQAAVFYEMQANLINSRLQQHLDHQNKIIQATLLPHSGPQK
                     TLELDSSVMLGILTNPQKEGVFAPHHTFVRNTAKALHEQFNLMFPINKNRSGAILFGR
                     YPGDTYDGYQTNSIGNPWFILTATMAEYYFTMAHNLSPNSSNKLHIQNYLKKGDNYLR
                     LIKQYGPDLNLSEQINLNTGVQQGATSLTWSYVSVLRAIHLREQLENRIKTMGWNTY"
     gene            complement(23412..24833)
                     /locus_tag="AC06IO_000455"
     CDS             complement(23412..24833)
                     /locus_tag="AC06IO_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HIO9873671.1"
                     /translation="MAWIVAIIGSIAGFLFGYDEGIIAGSLGLVKNHFNLNATHIGVM
                     ASALPFGALFGSLLIGAFMASKCVKRFGRCSLLSFAGFLFFVGALGAGFAETISVLIL
                     SRLILGLAIGMASVLTPLYLAETAAVQSRGAVVAIYQLALTVGIVCSYSVNYLLIEQQ
                     AWRAMFASSAIPALLLSLGILFMPESPRWLCSVGRHDAAANSLRKLRGKQSVEQELKE
                     IEATLANEPKQGNWLLLFQKPLLPVLMLGTILFCLQQLSGINVVIYFAPEIFKNLGLG
                     STTGQILATMGIGLVNLLVTIIAILYVDKLGRRKLLLLGFAGTSLSLFALSLFSLNHV
                     AWLSYLSVICLMVYIFSFAISVGPIPHIAMAEIFPLHVRGAGMGMSSMSNWSFNTIVI
                     FSFPVLHQMFGIEMTFVLYAVICFLGFIYAYIYMPETRNISLEQIETYIMSGKPLRFL
                     GREDEEVNAVSTKSESSLLASTN"
     gene            complement(24947..25609)
                     /locus_tag="AC06IO_000456"
     CDS             complement(24947..25609)
                     /locus_tag="AC06IO_000456"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIO9873672.1"
                     /translation="MSFSEWKTQPGAVFAASAVIPVIVIKELEDALPLAEALFAGGIH
                     VLEVTLRTPVAIKALELLNNTFPDVLIGAGTVITPSQFHDVVAAGARFAISPGQTREL
                     LIAGQKSEIPLIPGVASVSELMEGLGMGYNHFKFFPAAAAGGIPMLKAISGVFPQVKF
                     CPTGGINSKNYEEYLCLPNVACVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCS
                     QN"
     gene            complement(25596..26603)
                     /gene="glk"
                     /locus_tag="AC06IO_000457"
     CDS             complement(25596..26603)
                     /gene="glk"
                     /locus_tag="AC06IO_000457"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIO9873673.1"
                     /translation="MNFFMMPHDLAQYAIVADIGGTFARFSRVNLVNLQMDKIEISPC
                     AEFISLESALLTYKNRHALQEIKHIAIAIACPVIDDLVSMTNCHWQFSITELKQRLGL
                     EVLEVMNDFTAIAMSLPVLSTQDLVQIGNGYLDASKVRVVLGAGTGLGVAYLIPHQHH
                     YSAFAGEGGHADWGAKTEQEWFIYRYLKSKYSHVSYERLLSGQGLENLYQALAAYHSK
                     KVEFLSAAQIISLALNQECFIAHKVVAQFFSSLGSFAGDLALTYGAFGGVYIAGGIMP
                     RLLSLVHQSDFRIQFEDKGRFSDFNALIPTYVIAASQPGILGASVSLKQILTGASHVL
                     F"
     gene            complement(26578..28416)
                     /gene="edd"
                     /locus_tag="AC06IO_000458"
     CDS             complement(26578..28416)
                     /gene="edd"
                     /locus_tag="AC06IO_000458"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HIO9873674.1"
                     /translation="MHPVINQVTENIQNRSAARRAAYLNQIDKARTKGPQRSILHCGN
                     LAHGFAACAKQDKAILRGNAKANIAIVSAYNDMLSAHQPYAVFPDLIKEAISAAGGVA
                     QFAGGVPAMCDGITQGQPGMELSLLSRDVIALSTAVALSHNMFDGGLLLGICDKIVPG
                     LLMAALAFGHLPFIFVPAGPMPSGISNQEKARIRQLYAAGQVDKNALLDVEAASYHTA
                     GTCTFYGTANSNQLVIELMGLQLPGSSFVNPNTPLRNELTKAAACQILSLTDLNSDYM
                     PIGQMLDVKNIVNGIIGLLASGGSTNHTMHLVAIAAMAGYWVNWDDFAQLSAIIPLIA
                     RIYPNGQADINHFQRAGGMAYFVKTLLEADLLHPDVNTVVGFGLDKYTQQPQLIDKRV
                     VWVNGPDSSSNNEVLTSAADPFKPQGGLQVLSGNLGRAIIKTSSLYDEHYVIEAPAVV
                     CSSQEEFEHLFNQGLLNKNCVVVVRFQGPKACGMPELHQLTPKLGVLMDKGFHVALVT
                     DGRMSGASGKVPAAIHVTPEAVDGGGIAKIETGDMILIDCERGILQLLVSDEELAGRS
                     PVAIEGYSSVLGMGRELFGNLRLHFTGAEQGACSLFKGNEFFYDAS"
     gene            complement(28404..29117)
                     /gene="pgl"
                     /locus_tag="AC06IO_000459"
     CDS             complement(28404..29117)
                     /gene="pgl"
                     /locus_tag="AC06IO_000459"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIO9873675.1"
                     /translation="MQFHSFRDAKQLIHDMVEKLRTILSDAIKRRGQAFLVVSGGRTP
                     IDLFKELALADLPWEKVVITLADERCVNLFDPHRNELLVREFLLQEKAKKAKFISLFD
                     EKEDPLDHLNEVNSQIDSLPMFDAVVLGMGEDGHTASLFPCSDELSAGLDDKANAVLI
                     VKPRTASFQRVSLSKRRLLNSSTIFLHLVGQRKRVILEQAMSTNNPEILPICAILNAP
                     DANVQVMYAPSDEEKECIL"
     gene            complement(29104..30594)
                     /gene="zwf"
                     /locus_tag="AC06IO_000460"
     CDS             complement(29104..30594)
                     /gene="zwf"
                     /locus_tag="AC06IO_000460"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIO9873676.1"
                     /translation="MLLKAESKYPCDLILFGTLGDLSCRKLLPALYQLELANLLHEKT
                     RIIGCAREELDFSAYVDLIKNKIQQFLFDEIDEAVWLRFVKKLAYCKLDLNQSDDYQK
                     LTDYVNPSERVVISYLAIPPSLYSQTCRSLARLNLTQKPSRVVLEKPIGHDLPSSVAT
                     NEQVACFFAEDQIYRIDHYLGKETVLNLLVLRFANSIFSTNWDNRVIDRVEISVAEEI
                     GVEGRWEYYDKSGQVRDMLQNHLLQILSLVAMEPPLSLSAESIRSEKLKVLKSLRFIN
                     KSNVHDHTVRAQYVANTLANGKKIPGYLEEVGANQASTTETFVAIKAYVDNWRWSGVP
                     FYMLTGKRLPKKQSEVVIYFKPQPYNIFHPLNEELYPNQLIIRLQPDEGVEVRIMNKI
                     PGLGERMMLHDSKLDLNFHHSFKTQRIADAYERLLLEVMLGNQYLFVSREEIEQAWQW
                     IDSIVSAWEEDNIPLYTYPACTWGPKEVIRLFNGQAHVWGDHNAVS"
     gene            30912..31157
                     /locus_tag="AC06IO_000461"
     CDS             30912..31157
                     /locus_tag="AC06IO_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873677.1"
                     /translation="MLNKRFSERLNKELDNIGVPEATTERIEILSKLIKIPKFKAEAL
                     LNGITSPDEAVLKTLAQELEVNADWLIGKSDSKSGAH"
     gene            complement(31161..32069)
                     /gene="rimK"
                     /locus_tag="AC06IO_000462"
     CDS             complement(31161..32069)
                     /gene="rimK"
                     /locus_tag="AC06IO_000462"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HIO9873678.1"
                     /translation="MKIAILATNPHLYSHKRLKAEAEAAGHEVKIINPLYCYMNVAAS
                     NPKVHYRGGAPLPHFDAVIPRIGASITYYGTAVLRHMETMGMYTLNESIAISRSRDKF
                     RSLQLLARKGIPMPLTSFAQSPDDTEDLIHMVGGAPLVIKLLEGTQGKGVILADSHQS
                     AVSIINAFKEMHANILVQEFIEESRGTDIRCFVIGEKVVAAVKRQAKDGEFRANVHQG
                     GKAVKVKLSPQERAIAVSAAKTMGLRVAGVDLIRSNHGPLVLEINSSPGLEGIEKATN
                     INLAGKIIEYIEKKAKPISSNHRFHG"
     gene            complement(32435..33076)
                     /locus_tag="AC06IO_000463"
     CDS             complement(32435..33076)
                     /locus_tag="AC06IO_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIO9873679.1"
                     /translation="MNIKIKGGAVTITVLALVSLLAGIYISQNFHFKKKINVADFHGT
                     YLENPRTVNNFSLTGTDQKVFDNSSLQGRWTLVFFGFTNCGYLCPTTMAELGKMYRIL
                     EKKGVKNLPQVVMISIDPQRDSLDKLGSYVTSFHKSFYGARGEEDSIKSMTHEMGIAY
                     AKINNKESSEPENYDIQHSGAVMLFNPQGELNAFFTTPHHADLLAKDYILLVS"
     gene            complement(33086..33970)
                     /gene="cyoE"
                     /locus_tag="AC06IO_000464"
     CDS             complement(33086..33970)
                     /gene="cyoE"
                     /locus_tag="AC06IO_000464"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIO9873680.1"
                     /translation="MRTEYAAQLPVDWRDYVELCKPRVVLLMLLTVIVGMYLAAPGWV
                     SLRLIAFTLLGIGLCAGSAAAINHLVDRHIDSIMARTKKRPVAYGRVSVKQALWFAVI
                     IGLMGLSLLILFVNQLTALLTFVTLIGYAGVYTGYLKRATSQNIVIGGLAGAAPPLLG
                     WTAVTDQLDPQALLLVLIIFTWTPPHFWALAIYRYKEYQDAEIPMLPVTHGIQFTKLN
                     IYLYTVLLLVVSLLPFVVSMSGWIYLLGALVLGIRFLVWAHKLYFTDKPVVAMQTFRF
                     SILYLMLLFVFLLVDHYF"
     gene            complement(33960..34967)
                     /locus_tag="AC06IO_000465"
     CDS             complement(33960..34967)
                     /locus_tag="AC06IO_000465"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIO9873681.1"
                     /translation="MSSSSIRLAATLAVFLAFFVVMLGAYTRLTDAGLGCPDWPGCYG
                     RMVLPGADKGLQEAQSQYPDLPIESKKAWTEMAHRYAAGTLALLIFFIAFQVWFKRRQ
                     DQDLPWHLPMALFMLVVFQAALGMWTVTLKLLPVVVMGHLLGGMLIVACLCRFRLQIA
                     QLKGQNLPEWRPWLRLGIVIILLQIALGGWVSANYAGISCIGFPLCNGVWFPELHFAK
                     GFNLFSTIGANYQGGVLESEVRASIQFIHRIGAILTATYLLVLSLIIVFKTHSIYLKA
                     TAIIMAMLVLLQFTLGIMNVIHLLPIKVAVAHNGVAALLLAVAFCSLHFAQDREVSHA
                     Y"
     gene            complement(34972..35517)
                     /locus_tag="AC06IO_000466"
     CDS             complement(34972..35517)
                     /locus_tag="AC06IO_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873682.1"
                     /translation="MKKPYKYMTLLLLVVIFAAPGITAYLFYKHPSWLGTSKVNKGIL
                     LSPPIALKPLDSSAKWRIIYWNPNGCDKTCLKQLNMLGRVRLSFGRKLYQVDEWLLLS
                     NKDSLIAEELKPILKEQDIHVAALSLTEMSKLSTLYPEAKIFIANPDNYLILSYQPGC
                     NPGDIYKDLKLLLNTTEIKSG"
     gene            complement(35501..36229)
                     /locus_tag="AC06IO_000467"
     CDS             complement(35501..36229)
                     /locus_tag="AC06IO_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIO9873683.1"
                     /translation="MPSLTCFNFRFTSNWPMLILTAVCFFLFISLGFWQIHRADEKTE
                     MISAQQALAKQEPIIWQPGQKLPEQYQRISIEGVFLPKLFLLDNQHYQHQFGYDVVSP
                     MLLDDGSIVMVDRGWVSGDITRRTFPDVQTPNGKFKLFGMVYFPSKKQWVLGPSYEEK
                     ENKVTLLELIDEEILKQLLQKKVYPFIIRLDKNEPFGFVREWEIVSMPPQRHFAYAIQ
                     WFAMALVILIIFVIFNLKKNEKTI"
     gene            36297..36500
                     /locus_tag="AC06IO_000468"
     CDS             36297..36500
                     /locus_tag="AC06IO_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIO9873684.1"
                     /translation="MFTKIFIIFVMILIVASLASGLFYLVKDTDKSKRTVKALTVRIA
                     ISLSLFVFLFIAFKLGLIQPHGI"
     gene            36778..36921
                     /locus_tag="AC06IO_000469"
     CDS             36778..36921
                     /locus_tag="AC06IO_000469"
                     /inference="COORDINATES: protein motif:HMM:NF039621.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5993 family protein"
                     /protein_id="HIO9873685.1"
                     /translation="MIVLFLFYFIIGLQIIFKPNKTIILQFLFNLLLILISFNYHSHL
                     VNL"
     gene            36918..37466
                     /locus_tag="AC06IO_000470"
     CDS             36918..37466
                     /locus_tag="AC06IO_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIO9873686.1"
                     /translation="MNKQMQWHLFGNTSGLLIICCLLILAFLDQFIFYELPCPICILQ
                     RICFVAVGLCFVMNLMLGIRASHYGLMLLASLLGLSISVRQMYIHLTPGDPGYGGLFL
                     GLHLYTWSAIIFLVIILLIAGALILDKGLMPDYKVRSKSAKVMIWVFFILILANGIST
                     FLECGPHECPDNPSKYYLLNNN"
     gene            37485..37928
                     /locus_tag="AC06IO_000471"
     CDS             37485..37928
                     /locus_tag="AC06IO_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIO9873687.1"
                     /translation="MRLLFISLILASGYLQADTINNYMNIANNIPQMEMKADPQAQAW
                     ARSARHVLTITSESIAETLIQANEAAKTQGKPIFCLPQGVQLNSFVMNELIQQTYKEI
                     SSQQSDKDKMTVSQVALLGLSKKYPCEPSPQEKQIQHVASLLSPQ"
     gene            complement(38143..38484)
                     /locus_tag="AC06IO_000472"
     CDS             complement(38143..38484)
                     /locus_tag="AC06IO_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIO9873688.1"
                     /translation="MSDKFSHITKDITTQLAKFRKEMPELMTGFSSLAQAATKDGALD
                     KKTKELIAMALAVAKQCPGCIGFHSQTLVKLQATREELLETLGMAVYMGGGPSLMYAA
                     EALEAFEEFSK"
     gene            complement(38688..39278)
                     /locus_tag="AC06IO_000473"
     CDS             complement(38688..39278)
                     /locus_tag="AC06IO_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0405 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873689.1"
                     /translation="MLYNLTRQVKFNNPSCLCNFQKMAYIDELLEELSARLPELEWKI
                     NGLSSSISIHNLPKGIFSSVIEFNGPACIKEIQDDIHALQKHKDESIACFLAERIQKK
                     INVLVVLCQMDKKNNKPESKISFDLTTLSTRQQWIKTMEEKICALEEQYQAMRKTLEQ
                     MKSCSSPATILHLQTELGNVEKRLTLAKETLNRAIS"
     gene            complement(39268..40671)
                     /locus_tag="AC06IO_000474"
     CDS             complement(39268..40671)
                     /locus_tag="AC06IO_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9873690.1"
                     /translation="MSNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILG
                     ALFFLIPTALVSAELASGWARQGGVYIWVKEAFGKKSGFLAIWLQWIENVIWYPTILS
                     FVAGTIGYLINPSLTTNPYFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLP
                     MSLIIGLGAVWIAQGNPLQISFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHAN
                     DVKDPQHAFPKALVYSVGIILSTLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYH
                     MDWMMPVVAVMLVLGGLGGVSNWIIAPTKGLLVAAEEGNLPEVFQRTNGKGAPAMMLY
                     TQATIVTVLSALFLFMPSVNGSYWLLTALAAQLYMLMYLIMFVAAIKLRISQPYHPRP
                     FKIPGGMAGMLFVAGIGIIGVVTTLAVSFIPPEGINVGSATRYELTLIIGLLLMCAPP
                     FITSWLQAKEADLEPAV"
     gene            complement(41008..42516)
                     /gene="ankG"
                     /locus_tag="AC06IO_000475"
     CDS             complement(41008..42516)
                     /gene="ankG"
                     /locus_tag="AC06IO_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkG/AnkZ/LegA7"
                     /protein_id="HIO9873691.1"
                     /translation="MIYVLICIMLVCGLVVYQLMPKLLPYSTSFFSKKQDTSELTHDG
                     ITNVLTQLGHPRFEGVCYGFTLNWALAVAQGKESFFYRQLHHLRTQQLGLPETLQQIK
                     EKKERNQSLSKDEKIIETLPQLGKKICIAQDPLQYKEKYKKLVWQPDINSILKAINAD
                     SSVAKHIFYKTHSFLNQDEATEYLELLKRTGIREDVAVIISTADHAMGFKLAGNIWRF
                     ININDLYQQDKNKPYFEFSSRNLVKELYRVCAENLQGSRLTVNTDFVSIHPEEKLSSA
                     LQNLFPVFPVRTKTSYPERLAFFSMAATQGDMNSVKKCIHSGWSIFSRQRLSDDSPIL
                     TAIYLGRRDVVRAMLSTSRHRVNQKRKSDSSTLLHIACRYGGSGIVEDLLNIRGIKID
                     PQDSKGRTPLMYACKKSVVTEDRKLFNLLFAKGASLSIKDNDGLTALDHALKNEHTLA
                     IQMIEERLEKGACAQENSTSRRFKFSETKGTLFQRGVKTISYPSPQPGFGMK"
     gene            42777..43451
                     /gene="legA7"
                     /locus_tag="AC06IO_000476"
     CDS             42777..43451
                     /gene="legA7"
                     /locus_tag="AC06IO_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegA7"
                     /protein_id="HIO9873692.1"
                     /translation="MESLMPKAFTHLCKKVNLSLYNDTLTVYPKSSVLPKPIQIRSEL
                     DLLMVLLFSQMDCNSAQVDRFFMMRSNELGVYRFIHRDDTDHDLYHQDEFSMDENHYH
                     AQFDKTIDESKLENILGILEKHSLISSEEHVSFIEAYHKANTLPEDMPKLETTLPSPL
                     PVSSPSVHSGTKRPVVKEKEVDTPTTFAGLRVGFFLTSTKTTTKPLSRSHDDEKKTGL
                     DRHEFH"
     gene            43708..45084
                     /gene="ffh"
                     /locus_tag="AC06IO_000477"
     CDS             43708..45084
                     /gene="ffh"
                     /locus_tag="AC06IO_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIO9873693.1"
                     /translation="MFENLTERLTRTFKNLRGQGRLTEDNIQHALREVRLSLIEADVA
                     LPVIKEFIEQVKQKSLGQEVLTSLNPDQAFIKIVHDELIHVMGDERVELDYKTQPPAV
                     FLMAGLQGSGKTTSTAKLARYLKETENKKVMMASVDVYRPAAIHQLKVLAEQIDVAFF
                     DSDPNEQPIKIAQKALESARKQYVDVLILDTAGRLHVDEDMMSEIKSLHKAVNPIETL
                     FVVDSMTGQDAANTAKAFHEALPLTGVILTKTDGDARGGAALSVKQITGKPIKFIGSG
                     EKIEALEPFHPERIASRILGMGDILTLIEEVERKADKQASEKLAKKLKKGKGFDLEDF
                     KQQLLQMNNMGGISGMMSKLPGVGQLPKQAMQQVNDKAMAQTIAIINSMTPKERRIPK
                     IIVGSRKKRIALGSGTQIQDVNRLLKQFEQMQKMMKKFTKPGGMQKMMRGIGGMAGLK
                     GMFPDDLK"
     gene            45171..45431
                     /gene="rpsP"
                     /locus_tag="AC06IO_000478"
     CDS             45171..45431
                     /gene="rpsP"
                     /locus_tag="AC06IO_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIO9873694.1"
                     /translation="MVVIRLSRAGAKKRPFYHMVVTDSRKRRDGNYIERIGYFNPVAR
                     GQEVKLHIDMDKMTHWQKVGAQLSDRVSALLKEHSKKSETAA"
     gene            45437..45946
                     /gene="rimM"
                     /locus_tag="AC06IO_000479"
     CDS             45437..45946
                     /gene="rimM"
                     /locus_tag="AC06IO_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213051.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIO9873695.1"
                     /translation="MNNKTNWVIIGRFGRPHGIKGFVTVHSFTDPADNILRYNDWHVF
                     LNKQWQPVKLLTIEVRSKAIIAQIEGYPERELVSALTNLDIGVQESQLAALAPGEYYW
                     YQLIGMSVINSKGDLFGKVVEIMPTGSNDVLVVEGEKRHLIPYLPGQFVINIDESQQI
                     ITVDWDMNF"
     gene            45946..46698
                     /gene="trmD"
                     /locus_tag="AC06IO_000480"
     CDS             45946..46698
                     /gene="trmD"
                     /locus_tag="AC06IO_000480"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIO9873696.1"
                     /translation="MALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSS
                     RPYKQVDDKPYGGGPGMVMMYEPLHAAIKHARSEMKENCKTIYLSPQGKVVRQNDLKQ
                     IAAKKQSLLFVAGRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
                     SLGHLGSAEQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKT
                     WLKRPDLLEKVQLSETDKQLLAEFKCEHGDSC"
     gene            46725..47090
                     /gene="rplS"
                     /locus_tag="AC06IO_000481"
     CDS             46725..47090
                     /gene="rplS"
                     /locus_tag="AC06IO_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIO9873697.1"
                     /translation="MTNIIDQINAEQMQGKEIPDFNPGDTVLVQVKVIEGNRERLQAF
                     EGVVIAKRNRGLNSAFTVRKISHNVGVERVFQTYSPIVDSITVKRRGDVRRAKLYYLR
                     NLAGRAARIKEKLSGKKGD"
     gene            47106..47561
                     /locus_tag="AC06IO_000482"
     CDS             47106..47561
                     /locus_tag="AC06IO_000482"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIO9873698.1"
                     /translation="MLLISIFNTPVGNLEVAHDEHFIFNASFTDKSGTPAQNQLAHLI
                     AGELKAYFDNPHHRFQLPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRA
                     VGQACKCNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII"
     gene            complement(47661..48524)
                     /locus_tag="AC06IO_000483"
     CDS             complement(47661..48524)
                     /locus_tag="AC06IO_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0393 family guanine-nucleotide exchange
                     effector"
                     /protein_id="HIO9873699.1"
                     /translation="MLSSTKEYLQALRDGKYLLFLQWPKFIAEYYGKSDKNQEADEMV
                     SLLIFEWLNNGFCLDDIKKFAILYAVHEMESRPLREGLSYALTTISIALFPCMVYLTN
                     NLQEHYITSKKLSSKEVLQLMTMNNAYLEKQRFVEFLGQEQDKFFTWVKEADSSAVSK
                     AFDQIYSVTYLKYLIEDYLSLLESANLPTDQLKSSRISLVVRLAKYLHEQTELTQDVH
                     DEIAVYVKKLWEMQPAEFEEEFLKKISPLPFIDNTVRILTGLSARFFSILQPPSPPLN
                     INSDTDKNPKV"
     gene            48673..48954
                     /locus_tag="AC06IO_000484"
     CDS             48673..48954
                     /locus_tag="AC06IO_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873700.1"
                     /translation="MKNKLAVSILLFLLPTTAAMANCDLTKFRWDCDLPVQVKPSPGA
                     SSLVYCGSSYGYITKQQYDILARYQRASVNMVLDINGEYIDSPCIGAER"
BASE COUNT        15931 a         9842 c         8633 g        14646 t
ORIGIN      
        1 ttgaatcaat ggtatcaaaa aatgttgtac tggtattaac caataatttt aaaataatta
       61 gcgcaatgaa tcttatgctt atatgagata ttccacaaac attaaaggac gcatcttccc
      121 ttggggttta agaaaaatct caagatccag gtaaaaaacg ttttttagga gggaatttag
      181 ataaaccaaa tgattgaatt ataaaaattt tttaacaaag agatttagtt ttgcaaaaaa
      241 ttaaaagatc aattattttg attttacaag ccacggatag aggatttaaa ttgaagactc
      301 tcgtcataac aggaatcagt agaggtatag gcctggaaat tactagaact ttccttgaca
      361 aagattggac ggttattggt acttccacca gcggatattc tcctataaaa cataaaaacc
      421 tacgcacata caagttagac ctagccattt cggagcacat cgatgcattt gcaaaaaatt
      481 tacctaaaat tgatgtactg attaataatg ccgccgtatt gcttgaagag tggaatgaag
      541 aaaaaattaa tatgtcctta ttgaaaaaaa cctttgcaat caacctgttt ggtacagttg
      601 agttaactga aaaatgtatt tccaagttca atgatggtgc acaaatcatt aatatctctt
      661 cagggtgggg agcatttagt tccaataatt ccccctttca accacattat aaaatgtcaa
      721 aagtcagttt gaatatgtat actaaattgc ttgcggaaag acttccccaa ataactgtat
      781 ccagttttga tccaggttgg gttaaaacaa acatgggaac acaaaatgca aaaaaacttc
      841 ccagtgaaac agcactggaa atctatgagc tgattagtat gaagaaaaaa agcggctatt
      901 tttggtttaa tggaaaacca agggattggt agccagttaa atttcagtta ttctcaacta
      961 tgaaataaag catcaaagga gtgacaaacg tgagtttcga ttttataaaa tcaatatgga
     1021 ttgcagctct tataggtata acaccaacct tgtcttattc agagtccaat atccagtatc
     1081 aagaaacact tcaatcacat tgctttgaaa cctggatgaa aaaaatagat ccatcaattg
     1141 acaaagaaac atataaacaa tttggagata aatattgtcg atgcatttca actaaaaatc
     1201 tcgataataa aacaggggtt caaaaagcaa ttagaaattg cataccacaa acaattttac
     1261 atgatgccat ggatgaactg gaggaagagg tgactttatc agaagtaacg acacaaacaa
     1321 tcgaacaata ttgccttaat agatggagcc ttatttatcc caatcaaagt gatgaagata
     1381 aaaaaacaat tcaggcctat tgtgcgtgtg tcaaaacaaa attattaagt tttatagagc
     1441 aaatagaaaa aatatcaaac agggaataca atgaagccat taatgatatt aatacgattt
     1501 gctcggaaaa tttaaatcaa taacagagta taatataatt aaatgtcgac taaattaata
     1561 cacacattta gcaccagcaa gttctaatcc ttttatgaaa ggcctattca tgtcaagtaa
     1621 aaacagccat agcactttac tcaataagca agaaataaac tggattttag agcagcgtat
     1681 tacaagactg actgacaata aagaagtttt aatttatcca atgggagcag aacgctattt
     1741 atcatctgct ttatctggtc atgaagaaga aaggtataat ttacacagtg aaatcaaaga
     1801 aaaaaatgtc ctaataatcc ctggatatgg taatagcgct tttttatttg ctcaagcagg
     1861 ggccaaatct attcttgttt atgacaaaga tcctgtgact attgcatggg taaaagcatt
     1921 taaaaaatat taccattaca gagaatacaa taataatgga aaaccatatc cttcaatagg
     1981 cgaacttctg acagctctca ccaaatggta tcctcctcta cttaaactac cttcaggagt
     2041 gattaaaaac tctgtatttt ggataattaa ccctaaatta ctaagaaaat catatattta
     2101 ttatatgctg aaactcacca ggcaagctgt acaattaaga gttcaaaatg aatttgagtt
     2161 cgatcatcat atagagtttt atgcgggtga attaaaacat ctgataaaaa atcatccgtc
     2221 aaaaacattt gatacagtat ttgttcctta tctcttaggc gtacaaaatg gaatagaaaa
     2281 agagaaagat attgttagtt ttattaagca attaattgag cttgtaccct atggtcatat
     2341 tttagtgact ccttcaaaaa acactaaaga attccatctg gttgggcaaa gttacttttg
     2401 tacaacagct tattctaata tacagtccat tcctgattta aaaccttatg tcattgcaga
     2461 agacagtatt tggtttaaaa ctcaaggact tgcagtattt ggtataaaag caggtgcaga
     2521 gaatcatttg tgaaaataag gctatgattt attgtattag aaaacgtgct ttctcattat
     2581 ttatcaaaaa agggggtatt caccccccaa gttttaggca atatcaatat ctcgcgcttt
     2641 tccaaatcag gtcatgaaat aagaaatcaa tgcaattgat aagtattttc cttatttcca
     2701 tcagcaatta attgagttgt tctatcctgc atggttgatc ccccatgaaa agtcaaatgg
     2761 attggtaatg ggagatcttc ttttacctta tccaatccct tttttgccat tgttctcaca
     2821 tcagatgaag tagcattaga gtagattatt tttaaagtgc taatcatcat agcttgccta
     2881 tttacatcgt atttatccca ataagtaaat ttttcagtta gattggcagc caaagttgga
     2941 ttaattttat ccaagtccaa aatggcatca gcaaccagta ggtaaccttc acctgtgata
     3001 gaatgaaatc caaatgggtt ttttataaat gagcctaaca gagcatacac cttattagga
     3061 ttagacaaat caaaatcacc atgacgcatt aattttttta cccgctctat cacatcaggg
     3121 gagtgcgcca atgcttgaat actgaaccag ttatttacgg cgcccggatc atttttccaa
     3181 taatgataat aatcctctaa tgcaacatcc gcttcctcac agtttatttc gcataacatg
     3241 cttaaagcca aagcggtctc tgtcatattt ttgcctaaag catcctcaaa ttgaagaatc
     3301 agtttctcct ttgttttttc aaaatcaaca tttagcaaat aagaaaaaca tacggatttt
     3361 aaacgccgca atcccgcttg cttcaaatca aaacctttta atttttgttt atcatcatcc
     3421 ccagatattt gtattctaaa aaggagatta taaaaatccg ttttcaattc atttgcaaga
     3481 gcattttgta ttaattggcg cccctcattt aatttttcaa aatcaggctt atcctgattt
     3541 tcaatcaact cttctaaaga cggtaaggta attaattcag ccagcatcca ttcatttaaa
     3601 gagttatcag aaagtaatgc cttgtaaaca gcaaagaact gaggagataa ttctattttt
     3661 ttccctttgc aaaaatcgtt aagtaaagct gaaataagtt gctttgctgc ctcacaacga
     3721 ttatacaaat tagtgtcgaa ttgcatcaac aataataaat cttgataaga atattcatat
     3781 ttcatgtgta caggggcaga aaaactacgt aataacgatg gaatagggcg ggtctggata
     3841 ttttcaaatt gaaactcaat ttcctcttga tcaaggatta ataatttatc agctacaatt
     3901 tcttttcccg tactatcgag caaacccatc aggacaggaa tggggcgatt tttcccattg
     3961 actgttttaa tttttaaaag atattgcttt gtatctggat taaactcatc tgttactatc
     4021 aattcaggaa ttccagattc agtaaaccag gacaaaaagg aataaagatc tttatcagat
     4081 gagtgactaa gtgaatcgat aaaatcctcc agagtaactg cacccccatc attatcctta
     4141 aaaaacttgg ctactccttg gaaaaatgat tcttttccta cgaataacat catcattcga
     4201 aaaatatcag cacttttttc ataggctgcg gcagtatata agcttcttac tgctgtgtag
     4261 gcactttgcc gaggtgctct ttcgtccaaa ttcttgccat caagtaatct tatcaaatca
     4321 gtaccgaata gttcctctct gaacatagct gctctaaacg tagtaagacc ttcctttaat
     4381 ggtaaattga accaatctct tattgtgacc cgatctcctg accaataatg aaaaaattca
     4441 tgagcaacca cctccaacac acgcaaaata cccaagtcgg ttttagtctc gggagttgcg
     4501 aacaaatttt ctgtattaaa taaattcaag cctgttggtt cagaagcacc tgaggcgtat
     4561 ttatcaactc cggctaccat gtgttgtctc aaggcacatt ctaaattaaa agtacgctca
     4621 tcccatgcca ttgcttcttt caatacttct tttgcaaaat cacattttga tgtggctgat
     4681 ggtggaacat agaattcgat gggtaattca cggccagatt ttgtttgata ataagtgact
     4741 gatcgctgta aattgcctgc caccagggca aataaataac ttggctttgg gacatcatcc
     4801 agccaggttg cagaatgcag ccctaaagga agctcttttt tctcaattag cacaccattt
     4861 gaaagtaaaa caggataatc ttgctgattc gcgatgatag tagttttata agtcgcgaga
     4921 ttatcaggtc tgtccggcaa atataatact cgacgcaatc cttcagactc tgccttaacc
     4981 aagaccacac cctctgtttc atacaatcca aacaaatcgg tattttcacc gagcagagaa
     5041 gtcgtttcta tagtaaaggg agtattttga ggtatgtttt tgattattaa agcatcttta
     5101 tttagttcat attcgttttc tttcaacaaa ttatcgttca tttgtagtga gactaatgtc
     5161 atgttctcac catccaatat caaatcattt gaatgtgaat caatatttgg gttgggcact
     5221 actgtaagtc gggctttgga ttctactggc tttttactga gatcgacttg caaatcaaca
     5281 tggattacaa aataatcgag aaccttataa tcatttaatt tctttacttt agattgatct
     5341 attttcatga acactccctg atttaccaca aaaaactaag aattaatatt cctcatttca
     5401 actgattaca aaccatttat ctgctttcag ttcagtgaaa tcacgatata agatcaagat
     5461 agtaatttca atcaagttag tgtttttgtt atggcgatag atagtatcct ttgcactcaa
     5521 aggatacttg attaaagagc agtatgattt aaaaagagag tttaaattca aataccatga
     5581 tggttggtca aagaagttgt tagagttgtt tctggtttag ggacactttg aagtttcttt
     5641 agacactcat tgggatcaag actacattga ccaaaaagag agaaattata atcctgagac
     5701 ataccataac ccatgcatcg atctcctgtc gctacaatca gatcatgagc ataatcggct
     5761 agtttccttg ctgcctcttc atttcctcca agcctggtaa ctaaatcttt catcttcaga
     5821 ctgttaaatt ttcccttatc ataacatctc attaagtcca gatcatgagc cagagccata
     5881 agtataccag aggctgatga aattcctggt ttacccgtat ccagagcttc agcccaatat
     5941 tgaagttcgc tttcatcgga aaatagcgaa ggaattaatg acgatttatt ggtaaccaca
     6001 tggtttaaaa agcctttttg agcattttct ttgaaagtag cgtagatgtg tttggagtgc
     6061 tgcccattgt atgcctcctt aaatgcctgt tgataatgat tggcatctgt ccagctcata
     6121 tcgttttcac gcccaattac tgaaaataac atcatgtatt gcgctttttg tatttctttt
     6181 tgcgttaatt tttgaaattt tggatcaccc gaatatgctt tcaaaaattc agcaatgact
     6241 ggtacaagct gtgcaactcg taatgtatga gcaattccat gatttggcct gggaactaaa
     6301 cccgagggag tatttaatga ccaatcctgc cccatattgg accaatgttc ccaatcagca
     6361 tgcttctccc atgctgcctt tacattagca tctggttgtt tcagtttagg attataagga
     6421 tgttttaaaa aattactcca aacaaagtca atggccttgg agccattggc agatagtaga
     6481 taatcatttt tctctatttc tttggttatt ttggtattta ccaataatga agggccgttt
     6541 aaagaaagat tctcctgatg aaaatagaaa gtatcaccac ctgtttcatt ttttgttgga
     6601 caagttccta aatgagagct tactccgaga ttagacaaca caaagtctct aaattctgtc
     6661 ctcccaaaat taattgcaat ctccttattc ttggaaatat aacaacctaa tttattatcg
     6721 gggtatttcg aaggtttaat aagtaccata ttaggttctt tgatttcaaa aggatgagtt
     6781 gagatattga ctcctaaact ttttgcttta tctgttattt tgtttagaaa aaactctcgc
     6841 tccgtttgat tttgaaaagt aattcttatc atcggactgt caacgtttcc tatcgcacta
     6901 atagaaatcg aagaatcccc tgactttgga ggattaaaaa atgctggaga agtagtcgct
     6961 ggttttgtta cgggaacaat aacaggtgca ccagtcggta tgaatgttaa ttcagaattc
     7021 acacctggaa gcttatgttt atggaagaaa atagtattgt ggtccttgaa aatctcggca
     7081 tgttctgttt tcacgcccac tttaggtaat ataaaatgac tcaactccac ttttccaaaa
     7141 ttaacagcta attttccccc tggggatata taacaaccac aaggattaat tgggtaattt
     7201 gaagcctgca tcacaagaga atttccaggg ccttgcacta gtggaaaatc tagtcctaat
     7261 tcgcttgctt ttttctttac ctccttgaaa aaattacttt tttcttcctc actaataaat
     7321 tcaattttta aattaggttt accattatta gaaacccaaa ttattttcgt aaccataatt
     7381 ggcctctact attcccagta atatttgtga attatagtac attttgtcta ttaatttgct
     7441 atatggtttt gaataatgtc ataaaatcag gttagatgat ttaacggcta tgcctatttt
     7501 ttagatattc ttttaaggtg tgttgacaat tggctctcaa cgcctacgtg ccgcgacttg
     7561 ttcctgagag atggtcacaa aatgtgacca taactttttg aaaaaaatat aaattttttt
     7621 gaagtgcata atgaattgtg atcaaatagt gtttccgaca ttaaatgagc accatcatgc
     7681 acttaattga attattacat aaaaccttcg aagaaaaact acctcaaatc cataaaagta
     7741 gattaaatag tttactaacg gcttgcgatg cagcaattat tagcaacaaa ctttatttaa
     7801 caggactagg taggtcgcta tcaaatacga ataaagaatg cagcaacatc caaaaggtgg
     7861 ataggttatt aggcaatggt catcttcagt cagaacgagg atttttttac aagttaatga
     7921 cctcgcatgt catacaagag tatacctgcc catggattca tattgattgg acttgtatta
     7981 atccagtaac aaatctttat ggattgcgtg caagtgtctc tatgaaagga cgttcacttg
     8041 tgatttatga gacctgctat cctaaaaaac aagaaaataa tcatgcaacg cacaaagact
     8101 ttttaaatca attgaaagcc ttattgccgg tatcagtgaa gcccatcatt gttacagatg
     8161 cgggttttcg tgggctttgg tttaattata ttctcaaatt aggttgggat tttgtcggac
     8221 gtttacgtaa taaaaacgca gtatgtatgg gagacacatc tacgtggtgt ttaagctcaa
     8281 gttattttca actggccact tcaaaaccca cttatattgg ccatggtctc ttaaccgaaa
     8341 aaggaaaagt acctgcgcat tttgttgtct ataaaggagc tcctaaagga cgaaaaacac
     8401 tgactttaaa cactaaaaag caacgtgcat gtaaaaaaaa taaagttcac tccaaaggtc
     8461 ataaagaacc atgggtacta ataacctcct taaaaaaagc gtctttgagt ccatctctta
     8521 ttgtgaatat ctatcgccag agaatgagaa ttgaggaaaa tataagagac actaagtgtc
     8581 ctcattatgg attaggcctt aaagacagtc ttactcaatc tcctcaacgt atgaacattt
     8641 tacttctcat tggggccatt gctacttttg ccgcatggct ggctgggctc tttgttaaat
     8701 caataggaaa agctgctgat tttcaagccc aatcagctaa atttacttct gctttatctt
     8761 atgtttttct cggcaggagg gcgttgaaga aagggcttga tatcagaaaa gaagaattta
     8821 aaaatacatt attgatgctt tatcactgtg ctctccaagc tcagcaggag aatcctcact
     8881 atggttaata tttgtgagga tctctcaggg acaagccgag gcaaataggc aacgggatct
     8941 caaattatcc tctaacaccc ataaacattt ccatcttttg gggtatggtc atttttctta
     9001 tccccactct cttcgacttt cttgctgaag agtgaatggg ataggagtgc taccttgaca
     9061 tgctccttat tcacaagtgc cagagtataa gcggttgcat cattcatcgc atgctgcagg
     9121 aatccagcaa gctcagggtt ttctttaaaa tagctataaa ggttaacttg aacaccatat
     9181 cctttgaata tgtcttgtac ataaggatca tgaagaacat caatgacctc aagcatggca
     9241 tgaaaaccgc gcttggcata aacacctatg acctgaataa gaaaaagatt gatatcgttt
     9301 tgcagatcgg gatcttcctt ataattttcc atatacttgg ctaacatacc cacgatattg
     9361 caagtatgcc cggaaatcga tcctacgtaa gacgaatact ggcggttatt gttccaaaac
     9421 cattgttttc cctctttatt atcaggtgca acagttgaag aatccactag catattacgc
     9481 atgggttcgc gttgagcgtc ataaaagaca ggcgaacagc ctctcatgac tccgccacca
     9541 caattccata catccgaacc gactttttct gaatcagtct caaattttct ttctttaatg
     9601 cccgtcgatt tatcccgctc aaccctatcg cgccctttat gcgctccagc atgatgtaat
     9661 acaccggtaa taaattcgac ttctttcttg ttaaatttag aggtatcctt ataaccattg
     9721 gcaaagatac agattttttg ttccatctct cgtggagtat caactcttcc acaatcaccc
     9781 aaaaagaatt gcagaatttt attacatgcc ttcaatgtgt tatctctcaa ttcctcatct
     9841 tcaggctctt tatcttctcc tatcgaatca atttcaatgg tcttttctga gtgggaaaga
     9901 ccgttaatgt actgagcaag gcgagtttca aaaaaacgtc ttggatcatt tttcttggga
     9961 tcaaaaggct taaggtttgt agaagcttcc atgatttttt gaacttctct ttgccccaat
    10021 tgttctatca ctttcttttc ttttaatttt tcaattattt tatgcaagca gtcatcatat
    10081 cgaacaaata aataatcagc cgataattct tgcaaaaact ccttatctcc aatttttaag
    10141 gaagtaaaaa gaccaatcgt taatattaaa aattctgaaa gatttgtttt gtcaccaaca
    10201 agagacaagt atttgctcaa gtttatgttc gttttctgat agctgggatc accaatcgat
    10261 ggacgaaatg cgggtaatac agagtcaaat gagatatcag ttaattgtga catgattgat
    10321 tttgcaattg aagtatccgt tttttcaatt aaaagaggca gtaactcttt tttagttttc
    10381 atgcttaaag tccctgtctt gctatcaaga gacaactcag ggatagactg gaaagcattc
    10441 atcagaatag tgagcaatac tttctcactt tcattggcaa gcatctcttg cacaaactcc
    10501 agaagctcag gtgagagcgg atgtgctttg tcctgactaa aagcaccgag catctccaga
    10561 tcacttgagt aaggtattaa ttcaatttgt cgtataattt cttcaagtag aggtttatta
    10621 tgagtcaata gcccttttaa gaagagagat tttaacaata tatgcttgga tttcggtgtt
    10681 tcctcaattg cttgccaaat ctgcttaaat cgagaaaaac gtatattttt cataaaatca
    10741 tctaaaattt tttgggtgtc aatagtgctg actgtagtat ctacggagaa ttttttggtt
    10801 acttcattaa ttttttgaac cgtttcatcg gataccctaa ccacatcttc agcaactttc
    10861 cttgataaaa cacttagctt attagctgtt tccttcgaag tctttttcag atcttccccc
    10921 agtttcttta gaatctcatc taagttttgc atataaactc tacctcttaa cattgtcttg
    10981 cctgacaact tatatttcac agtgggtatg ataaagcaca aagattaaga aaatattaaa
    11041 cttattgttc aaaattaaaa aaaagtgtcg tttatttaaa ttaccacctg aattgatcta
    11101 aataatcatt aataagtaaa ataaatgaac aaaaatattg tcatttatac agccttcatg
    11161 ctaagattca gccagtttga tttttagaat actatggcga tagtttaaaa taggtgatta
    11221 caatgggtag aagtgaaatg aaaatagcat cagctgaatt gcgtgaattg atgaaggcag
    11281 tatctgaagg tcattatgaa actgtcaata caatactgga taaagatcct gagcttgtaa
    11341 atcagtacgt cccccccact tacgattcac ccctggcaag agtattaaat aaaaaacaca
    11401 ttgactacaa aatgcttgat attttagtta agcaccatgt tgattttgat tatccaatca
    11461 accatcacaa agaaacacca attgaattgg cttgtcataa tcaggatctt caattattca
    11521 aatacttagt gcaacacaac gcccccattt cagagcatgc accacatttc ctgctggtga
    11581 atagtaccaa tatcaaatac ctgacagaag ataaaattaa aaatacctgt gaaattatta
    11641 aattaatggg tggtttagaa gctgtctctt ctaaatgtaa tgcggaaggt aatcgcttcg
    11701 aggaactggc aaggaaatca caattaatca atcgtttcgg tggtatagta aggtacgatt
    11761 acatgcaatt gctgcgatcg gtttatccaa ttgttgatag agaagtggat gttccaacca
    11821 ttcatgcctc tactgaaaaa cttacagacc tgcttaataa aatcagggat caacacacct
    11881 caaaagaagg atacgatcaa caaaatttaa aagattctat cagccttttc tttatgaccg
    11941 gtggagaaat acctccttca agaaaagttc cagagagttg ttttgaagaa gctggcatag
    12001 acgcccctaa aaacacgctt taaatttttt tatcccatct attaagtcgt caagaagcac
    12061 caaatttact atttggtgct tcttgacacc cccgcatctc tcctttatat aattctcaac
    12121 tcataagaac ttgattgtgc cttccaagcc aatacatacg attttatgat cacccattta
    12181 tgcaattttt ttggctacaa taaaaaattt atgccaatgc ttataataac ccaaggcatc
    12241 catttcatcc ttttcttctt cttgaaaata ttcaattttg aattgggaaa acaaatggca
    12301 aatcatttcc cgatttaaaa aagtcatatc cgttctggta ttccagaagt cattaaaacc
    12361 aaaaaacgaa ccagaaaatc gaccacctgg cttgagccct tttaaaataa cgttccagaa
    12421 tttataaaag taaactggct tgagaaatgg aagactaaag ttggcgttaa tcaagtttac
    12481 tttagggagt gaatccaaag cttcaaatga agaaactatt gtttttagtt tgttcgcgca
    12541 tgttaattct tgagctgaag aaagcaaact gtctatagca gataattgtg aatcaatggc
    12601 aatcacagac caaccgcatc gcaaaagttc cataacatcg ataccagcac cacatcccaa
    12661 atcgattgct gatttagata gattgatctt ttccttatca aaatttgcaa ttgctttaag
    12721 caaagacttt ctggggagag tcttttgttt cgtagaatta taataagccg tccaatttct
    12781 tttattcatt tactatatcc cctctagtta ccaaaaggta actaattgac tgattttatt
    12841 aaatatttta gtctcgaacc tgttcatttt ataaaataaa ggagtggatt atgaaagaaa
    12901 atctcattaa aaaaattgtc cagaaaaaac tgctaaaaag gaatagagga tggtgtggtc
    12961 caggaacttg tattggatga tattactgca acgatggcac gttatcaaag aacgtgccgt
    13021 tacacttaaa ccaatcaaag ccccataatc atgcatcctg acttaaaaga cttacttgat
    13081 tacagaaatc ataaaattat taccagatat aatatggatt acccgactgc agccatgcaa
    13141 gctgaagaag cattacatga attaatgaaa tttatttggc tctgccataa acataaaaca
    13201 gataaattac tttatcctga tgataaaaat ctggatttct cttgtgtcat tcatgctgaa
    13261 atggctgata ttgacaagat gtggcatact tttcttttat tcaccagaga ttatcatgag
    13321 ttttgtgata cctgtctcaa tggaaatttt tttcatcatg accctttaat tggggaaaat
    13381 acccctgttt ctgaagagga ttatgctctg gagttgaccc gctatttaac ctatatccat
    13441 gaccatttag gttcggaaac tttaatcaaa tggtttcaat aaatcaagca tgacaaccaa
    13501 gcttctgttc gcaaaatgtt ttttccgcat tgattatttt atccatattg gtttcagtcc
    13561 aacgagtaag taaaaccaat atggcactta attccttacc caatctggtt aatgaatact
    13621 caaccttcat aggtaaaaca ggataagatt gtctttggac aaagccatat cgctccaatt
    13681 tcagtaatgt ttgcgtcaac attttaggtg atattccacc gatttcacgt tttaactctc
    13741 caaaacggta tattttttgg gataaaaggc taatgaccaa tatagaccat ttatcactta
    13801 taccttcaag tacattttgt gatgggcact tctcgttata aacactataa tcaggatgag
    13861 acatatttgt actccctatt tctaattaat aacgaccttc aaactgaata aaatgggttc
    13921 gagctttatt catcagtaac ttaaatcgtt gcaaatgctg ttcagatgcc gttgaggaat
    13981 gtaaaacaaa ctctgaaata cataatgcaa aaaccaatcg tcgtatttcc caaatcgttt
    14041 cttgaaaaat atcaattctt tttttagtat gttcacttgt tttaaaatcc agtttcaaat
    14101 tttcggagct gatggatatc gggagataat gctcggtgaa ttggccacgt aatttctcta
    14161 caaagagcaa tgtattaatt ttttctgtat tggattcgtc tatttttcgc acaatatcat
    14221 ttaaaaaatc ataaactatc acctgatgaa aaaaataaag cctatgagaa taacggtatg
    14281 cctgaaataa aaaataatcg cctgccaaca caataaaaac accaaacagt gtacatatgc
    14341 ctcgattaag aaccatctca aaaggaccac gaggcgatgt cgcctcttcc gtttgcgcca
    14401 tcaaaaaaaa gacaactaaa gtaataaaaa atacactaat gtcgtaacgt cttgtgttta
    14461 aggtagcaac actcaaacca actatcgcca gaacaaataa gattgatatc aatcgatagt
    14521 tcaattgcaa taaatagagt aagggaatca aaatgatgag agccaacagt gtccctccta
    14581 tcctgtgttt agctcttctg ataatcaatc ctggttcaat acccgcactt actgacaaga
    14641 cagtgatcaa tacccaccac ttttcgggaa aagtcgaaaa caaaaaaatg agcatggcaa
    14701 ccatgaacat gatgactaga tgaataaatc gagcataatc aagttttttc ttcaatgctg
    14761 aagtatcatg cataattgat tccattggct aaatgcttgt tgcaaacatc tcataacata
    14821 ttgttgtaat tttgactcag gcatgatgga cattttagga gtgccacagg atgtgttggt
    14881 tttaatcgca gatctaagag tataaagatt ctctttcact ccatgccaga aagcctcatt
    14941 tttggcttca taataaagat tatccaaaga aaattgaatg ttgcgtatgt tgattgaaat
    15001 tcgctgagta taaatcatgt attttttggg gataagtcta cgatattgcc taagcatacc
    15061 caaatgagtc atttcttcct gcatgaattt gggaccacgg tgagtaaaag aaccttcaat
    15121 atccagctca agggattgaa taaattttgc caaagccctt ttccagactc cgagatattg
    15181 attggggaaa aatcttaaac aaatgaatat ggcgatcata gataatgcgc ttgacccaac
    15241 gatgtcgtag acaatttgcc aatttgcagg aggttctgta cttaaaattg tcgctccaga
    15301 agcaataatg agcatagtca cattcctcaa tggaagataa ttttttagca cataaaaata
    15361 aacgcttgct aacgtaataa ttgagaaaaa gaaaaataca acccgaaatg ggtaaacaag
    15421 ataaaatgag gtactcaaca ataccactac cactccaata aaaatcaaaa agtgttcttt
    15481 ctctttaaag ctggtaagac tgggtgattc ataaaatctt acgagcaaaa atggtgaaaa
    15541 aaatgccgta aaattcgtgg gacgaaatac ccaataaaca taggccatta cggtggcaat
    15601 aaaaagtgat ttatagagtg ctattcgttg aacagcataa ggatcaacct catcaatcca
    15661 ttcaataaac ttcgacatag acataagcgc tagctccaac acgaagcggg tatttagggt
    15721 cagggtcagt aacctttata atcacaggaa ggcgctgagg caataaaatc cattgatttt
    15781 cattgataac attttgcagc tgagtacgat catccaccaa ttgcctattc gcggaccaat
    15841 aatcagaatc aataacacca tgaaatattt ttcttcctaa atacattctt ggaaatatca
    15901 atacctttga gcccttgcgc acatcacgca aatccgtctc gttaaaattg gcctgaatat
    15961 aaatattatc ctgatcaacc aatgagaata atggctgatt gatattgact ggagtaccta
    16021 tggtaaaaaa caaattttga ataactccat taccctgagc ataaacatcc gttaaatcca
    16081 aattgacttt ggcatttttt aaccgggcct gaatggattt tatttcattc tcctgaactg
    16141 ttatttgatg ctgatctatt tcaagttgtt ttaatgaagc ctgccattta tctttagctg
    16201 cttttgtttc ttgctgggag ttttgcaggg taatcagaga aacagatttt atagcatacc
    16261 ctttaaggta tttttgatca tcttgcgcca actttttgta tattttttgc tcattttcgc
    16321 taagcttcaa atcccgttcg tacgtagcct ttaatactgc caactttgcc tgtgctccag
    16381 ctaaatcagc attgagctgt tcaacagtgt attcataggg ctttttaaat acggtaaata
    16441 gtttttgtcc cttacgcact gcatcaccat tttttacata aagctcggtt atatatccgc
    16501 ttgttaatgc tgcaaccgga cgtacattgt ttactacaaa cgcattatca gtaaaaggga
    16561 tcaaatagga aaaaacatag ataattgcag taagaaaacc gataaaaata atgacgttag
    16621 taagagtaaa ccagcgtttt ggttttaaac gaagcaagta ctcttttatt tttccaccat
    16681 attccatcaa ttttttcata aaatagccaa ccagtttccc ttggtatata acaataatgc
    16741 gaaattaact tatctcatcg aacagctcaa gagacgagaa ctgcaatttt agcccgcctg
    16801 gcgactttta aaatcaatgt ttacaaacca aactgattta gtcactacag gcatacccac
    16861 cagccaaatc ttgatacaac ataaccagtg acatggccaa ttccagctta gcctgatttg
    16921 ttgagagcgc cagggtatcc agataaacct tactctccaa taactcttta taggacatta
    16981 accctgattt taataaacca ttttgtaatt tgtatttatg ctgataatct tcttgtgcgt
    17041 gccgataaga taaaaattgc tctctcatgc gtttattagc tgaaaaagca ttatcaactt
    17101 cttttaaaat taatcggact gtcttaacgt attcggccaa ttttgcatta taaaccccct
    17161 tgctaactga aattttaccc agagtattag gattaattgt ccagtttacg tacgcatcgg
    17221 tcgcttgttc aaacgtacta tccggcaaat gtgcctctcc aataaaatca tccaactgca
    17281 taacaggaaa aaattcacta taagcaacaa aaatacccgc acgagctgct tttagggaat
    17341 actctgccat cttcatatca gggcgattac ctaatacagt agcgggtaaa cttccgggtt
    17401 taaagcgggt aaaatccaat tgagcaaaat tatttttgct tttgattaaa ccaggattct
    17461 catttattaa ataacgcaac gcattctgac taacaacaat attgtgttta atgggtttta
    17521 tttgtgctgc aatcaagcgc tcgttggatt gcaactgagc caaatcaata tcatttttta
    17581 atcctatgtt gatttctttc ctgctcaaag caattaacga ctttaaatcc ctatccagtt
    17641 tatacaataa tttaagttgc tccaactgtg caatcaaagt gaaataagcc gctgccacct
    17701 gaccaatcaa ggtcaaacgc aaaccatcga ttgctgcctg atgatattgg acctgatatt
    17761 gagcacgctt ttgctgagta taaagttgca taatattcaa aatataactt ggccatattc
    17821 cataaaaccc tcctggcaca ccaagagcag gattagttga ataccctccc aatatttgaa
    17881 cattaggtat ccaactcaac ttcacttgct gtaactctcc ttgagcttgc tgtaaattag
    17941 ccattgcaat atgaatgtcc atattggtct tcaatccagt atcaattaaa cgatccaatt
    18001 ctggatcttg aaattgcttc caccaggcca aataaggcaa ctcctgaact ggtcgataat
    18061 ccctgggact ggaggggaat tgcttcggtg ttataatatt ggaaatttgc tgatcagtgc
    18121 tttgaccaca gcttaacaaa cctgctccca acaataataa gaacattaat tttttaagca
    18181 tgcacatcat cgtttatttc aaagtgaatg tttatcattg taatttataa ttcatctaat
    18241 ggtgaaagta tatttaaatt tcaacaagga gctctcatga tcaaaaaaat agcatttacc
    18301 atgtatccag ctcaaaatat ggatagagca aggcaatttt atgaaaaaac acttggactt
    18361 aaagtcggta aaatttccgc tgagggagca tgggtagaat atgatttacc tcaagggggt
    18421 tgttttgcaa ttactacatt ggctcaagga gttatcccca gcgctacttc cggaggtagc
    18481 gttgcttttg aagtagataa tcttgatgag ctggtattcc aactaaaatc caaaggagtt
    18541 cagttcaaaa ttgacctatt cgcttcacct gtttgcagaa tggcgattgc tgtggattca
    18601 gagggaaatg cgttcacttt gcatcaatta acatgattgt tgctgccaat acgtgtgaga
    18661 atccctaaag agggccccac acgtattggt gttatgagag acatcaatat ttccaaaaat
    18721 acaaaataat aatgttcctt agtatgcctc gtttactaac aggacttacg atgaacctca
    18781 aggtcgaggc aaaaaatgtt tttaatgagg gagttgagat aaactaaatg actgagttaa
    18841 aaacattttt taacaaagag attggggttt atcgtaagcc ctgactaaat cacccaaggt
    18901 aggcagcaga ctctttaatt cactttcagg gtaaggaata gaaggcatgg aagattgcca
    18961 aataccttga ggccacaaag gatcattttt tctgcgaata acaatatgga tatgcaattg
    19021 cggaacaata ttgccaagag cgcctatatt aagcttgtct ggtttaaagt aatcactaac
    19081 cagaagagac agttgattga tttcctcaat taataaaaat cgctcgctct tttcaagctg
    19141 ataaatttct tgaatatcca tttttcttgg aaccaaaata aaccaggggt atgaggcgtc
    19201 attttttaaa agaactgtag ataaaggcca ctctccaagt ataaaagaag acgacttaat
    19261 ctgatcatca atgcaaaaca aaatactctc tctgtcaata agtactcaat cattataaca
    19321 aaaacaagac cttcagatta taagaattac agataaattt taataaacct attgagtaat
    19381 gatccctttt tgcgttatgt tgaaattacc tattgtaaaa taggctatta acaattgtca
    19441 tttaaggaag aatgcaattg ctcagctgac cgcttatgtc agtcgacgat tgataagctt
    19501 tagcataagg aggattcatg gctagaggcg aagtcaagtg gtttaataac gccaagggtt
    19561 ggggttttat tatcccggaa ggtggtggtg aagatatttt tgttcacttc tcctcaattc
    19621 acggcacggg atacaaaacg ctggttccag gacaggcggt cacctatgat gtcgttaatg
    19681 gtgaaagagg tttacatgcc tctaatgtcg ttgctcttag tgaaaacgcc gaacaagaag
    19741 aaatagctta acccaattaa tgcgataaac tattaagcgc ggcagtataa ctaatattta
    19801 tgctgctgcg tccatcaact tgaggtatta tgtccttcat tttataactg gactttagct
    19861 gatgagacgt ggtttattac tgttaaatct tggcactccc gataatgcgg atatcagggc
    19921 ggtaaagtta tatttgcgtg aatttcttac cgacaaaaga gtaatagact taccaactat
    19981 cccgcgctat atcctggtat attgtttgat cttacccttt agatcgccaa aatcagccca
    20041 ggcttaccaa tcaatatgga ctgaaaaagg ctctccactt ttgtatcata gccaaaatct
    20101 agtgacaaag ttacagtccg cacttaagga tgagtacaaa attgctttgg gaatgcgcta
    20161 tggaactcca tcaattacaa cagcacttgc agaattaaaa gattgccatt cccttaccat
    20221 tttaccctta tttcctcaat attcttctgc tgctacaggt tcagccattg aaaaaacctt
    20281 atcctatttg gcaaaccagg aaattattcc atcaattaaa atcattcgag atttctacca
    20341 aagaccagaa tacatccagg cgcaagctaa aattatgaag ccttatatta aagacaattt
    20401 ccacgttctt tttagctacc acggaattcc cgaacggcat attcacaaaa gcgggtgcga
    20461 tacgctttgc ccccaaacat gcacacccat atacgataaa aatcaagcct gttatagggc
    20521 tcaatgctat cagaccagtc tattactggc aaaagaattg caattaggca ctcaccaata
    20581 cacaaccgcc ttccaatcca gacttggtaa aactccatgg ataaaacctt ataccgatga
    20641 aatttttgcc gaactaataa gcaaaggaat taaaaacatt gtggtctcct gcccatcttt
    20701 tgtcgcagat tgcctggaaa ctcttgagga aataggaatt cgcgcaaaag aacagtggga
    20761 aaaacttggc ggtgagcaat ttatattaac tccttgcatg aacgatcatc ctgagtggat
    20821 caaagcaatt caaagtattg ttaacgagca attttaaccc gccgtcttac tgatttcacc
    20881 atcggcagtc ataataaaat tatgtttcgc attaaatatt tattgcaaaa caataaatat
    20941 ttaataaaat acgcatgatc tcatcttaga aggataatta tataatgaaa aaaataaaat
    21001 ctgtgagtca tttgcttttt ctattattca aaacattagc ctatggcctt ccccttgtaa
    21061 cgacttattt cattctattt catttcgaga gcatgctgaa ttggggggga tggcactccc
    21121 tgttatcaac ccaagtgaaa gacccttctc atttttcctt gttgcatcgt ttcattattt
    21181 tattgattga agcattaccc ttatccatta atgttctaat ttgtcatcaa ttggcaaagc
    21241 tgtttcgctt gtatgaacat ggctatttat ttgaagaaga aaacattcga ctgattaaaa
    21301 atatcagtat ttacctgatc tcaggtgaac ttatacaatt gctttatcaa cctttgataa
    21361 ccgcagctct gacttttacc aatccatctg gtcaacgtat cgcaagcatc accttgggaa
    21421 cagcaaacct gtccacgtta attaccgctt ttatcatttt gattgcctct tggattatta
    21481 aggaagcaca taaattaaaa acggatgcct tgttaaccat ttaatgtgaa cttcagcgta
    21541 aggacatcaa gagattttct tacgcagtct caatcagttt atatcccgct tgtttaagga
    21601 gtttcaatcg atgtcaatta ttgttaattt ggacgtgatg atggcaaaaa gaaagtgtcg
    21661 acttagagat ttagctgaag ctataggcat tactgaagct aacttatcca ttttaaaaaa
    21721 tggtaaagcc aaagcaatac gttttgcaac gcttgaggct atttgtgctt acttgcattg
    21781 tcaacccgga gatcttcttg agtatcaatc cactgaagac aatcacctta tcaaagacag
    21841 ggcttaatct gagaaagctt tgagtaagga cttgcaaaaa accagggtca agacaaaaag
    21901 taattgatgt taaacacatt ttacaataat ccgccctaaa gactatggtc tgaaaaactc
    21961 atttcctgta ctatggcgac cttctttttt aaaactaact actatcagga gattatgttg
    22021 aaaaggatct ttttcttaat gatttttttt gtctctcaaa ctatggcttc agtatttacc
    22081 catgaagagg ttcaaatatt aaaaaaacat tttttaaata actttcaaac caatggagcc
    22141 atcgtagcct ctccctccca atacaacccc aattattatt atgactggat tcgagactca
    22201 gccattgcca tgggattggt tgagacatgg tatgaagcca gccaatctgc tcgatataaa
    22261 aaacttttat tagaatacgt ttcatggaca gaaaaaatac agcatcaagc ggatcccatt
    22321 gctggccagg atatattggg tgaacccaaa ttttatatca atggcaatcc ttttgatggg
    22381 gaatggggac gaccacaaaa tgatggtccg gcactcagag catctgtttt aattcgtttt
    22441 gcgcaacggc ttctcgaaca gaatgaaata gattatgtta aatcacatct ttataacaac
    22501 accatggacc cacaatccat gggcactatc aagatggatt tggaatacat cgcacatcat
    22561 tggcaagatg caaattttga cctgtgggaa gaggtttatg gccatcattt tttcacggct
    22621 atggcacaac aaaaagcgct gaccgatggc gccattttgg ctcaccaact tcatgacaga
    22681 caagctgctg ttttttatga aatgcaagcc aacttaatta acagtcgact gcaacagcat
    22741 ttggaccatc agaataaaat aattcaagcc acattattac ctcactccgg tcctcaaaaa
    22801 acactagagc ttgattcgtc cgtcatgttg ggtattttaa ctaacccaca aaaagaaggt
    22861 gtttttgccc cacatcacac ctttgtgcga aatactgcaa aggctttgca tgagcaattt
    22921 aatttgatgt ttcctattaa taaaaaccgt tctggagcaa tattgtttgg tcgttatccc
    22981 ggcgatactt atgatggcta ccaaacaaac agcataggga acccctggtt tatcttaaca
    23041 gctacgatgg ccgaatatta ttttaccatg gctcataatc tgtccccgaa cagcagtaat
    23101 aaacttcata tacagaacta tctaaagaaa ggggacaatt acctaaggct gataaaacaa
    23161 tatggccctg atctgaattt aagtgaacaa attaatttga ataccggagt tcagcaagga
    23221 gcgacatcct tgacctggag ttacgtttcc gtcttgcgag ctattcattt aagagaacaa
    23281 ttggaaaata gaataaaaac tatgggctgg aatacctatt aggattttat acgttcgggt
    23341 aatgcgataa agaatagaat ttgtggcccg tgtcagtcaa agataacacg ggcatcatag
    23401 gaaaatttat atcaatttgt tgaagccagc aaagaagatt ctgatttagt tgacacagca
    23461 ttaacctcct catcctctct acctaaaaac cgtaatggct tgccactcat tatataagtt
    23521 tctatttgct ctaaactaat attcctggtt tcaggcatat aaatataagc ataaatgaac
    23581 cccaagaaac agattaccgc gtacagaacg aaagtcattt ctataccaaa catttgatgc
    23641 agaacaggaa aactaaaaat aactatcgta ttaaacgacc aattactcat ggatgacatc
    23701 cccataccgg caccacggac atgaagggga aaaatttctg ccatagcaat gtgggggata
    23761 gggcccacgc tgattgcaaa agaaaaaata taaaccatta agcaaataac ggataaataa
    23821 gatagccagg caacatgatt taatgaaaac agcgataatg caaacaagct caaactggtt
    23881 cctgcaaaac ccagtaagag taatttgcgt cgccctagct tatcgacata caaaatggca
    23941 ataatagtaa ccagtaaatt aactaaccca atccccattg tcgctaatat ttgtccagtc
    24001 gtgctgccca agcctaaatt cttgaatatt tctggtgcaa aataaattac cacattgata
    24061 ccgcttaatt gttgtaaaca aaataatatt gttcctaaca tcaacacagg caacaagggc
    24121 ttttggaata gaagtagcca gttgccttgt tttggttcgt tcgccagtgt ggcttcaatt
    24181 tcttttaact cttgctcaac tgattgtttg cctctcaatt ttcttaagga gtttgctgct
    24241 gcatcatgac gtccaacgct gcataaccac ctgggagact ctggcataaa caaaatcccc
    24301 aggctcaata acaaggctgg gatagcactt gaagcaaaca ttgcacgcca cgcttgctgc
    24361 tctatcaata aataattgac tgaataagaa caaacaatac ccacagtcaa tgcaagttga
    24421 taaattgcaa ccactgcgcc acgtgattgc acggctgctg tttcagccag gtataatgga
    24481 gttaaaactg aagccattcc aatggccaaa cctaaaatta atcgagataa aatcaacact
    24541 gaaatggttt cagcaaaacc ggcacccagg gctccaacaa agaacaaaaa accggcaaag
    24601 gataataaag aacatctacc gaaacgtttt acacactttg atgccataaa tgcaccaatc
    24661 aacagcgaac caaacaatgc cccaaaaggt aaagcagaag ccataacgcc aatgtgggtt
    24721 gcattcagat taaaatgatt ttttaccagt ccaagagaac ctgctattat tccctcatcg
    24781 taaccaaata aaaaaccggc aattgaccca attattgcga caatccaagc catttttcac
    24841 tccttgattt taaattcttt tcctctgtct ctactctaca caatgaactg ttttagctgg
    24901 cactatccct tacatcacat aaaaacgctt tttcaacccg aatagattaa ttctgcgagc
    24961 aaagttctcg cttctgagaa ctcaccgcca tacataattc tgtaattaaa gaccagttat
    25021 gattcttgat agcctcttcc ggtacaatcc aggaccctcc aacgcaagct acatttggca
    25081 agcaaagata ctcctcataa tttttggaat taattccgcc tgttggacaa aatttaactt
    25141 gggggaatac gcccgatatc gctttcaaca tagggatacc tccagccgct gccgcaggaa
    25201 aaaatttaaa atggttatat cccatcccca aaccttccat taattcagag acactcgcta
    25261 cacctggaat gagaggaatt tcacttttct gtcctgctat taataattcc cttgtttggc
    25321 cagggctaat ggcaaatcga gcgcctgctg ctaccacatc atgaaactga ctaggggtaa
    25381 tcacagttcc ggcaccgatt aatacatctg gaaacgtatt gttcaataat tccagtgctt
    25441 ttattgccac tggtgttctt aatgtcactt caagaacatg aattcctccg gcaaataaag
    25501 cctcggccaa aggtaacgca tcctctaact ctttgattac aataactgga ataaccgcag
    25561 atgcagcaaa tacagcgccc ggttgtgttt tccattcaga aaaggacatg agaagctcct
    25621 gtcaatattt gttttaaact gacgctcgcg cctaaaatac ctggttgaga ggctgcaatg
    25681 acataagtgg gaattaaggc attaaaatca ctaaatcgtc ctttgtcttc aaattggatt
    25741 ctaaagtcac tttggtgtac caaggagagc aaacgcggca taatccctcc tgcaatataa
    25801 acaccaccaa aagctccata agttaatgct aaatcaccag caaacgatcc caaacttgaa
    25861 aaaaattggg ctactacctt atgagctatg aaacattcct gatttaatgc taaagatata
    25921 atttgcgctg ctgacaaaaa ttcaactttt ttactatgat acgcggctaa agcttgatat
    25981 aaattttcca gtccttggcc agacagcaag cgctcataag atacatggga gtatttactt
    26041 tttaaataac gatagataaa ccattcctgc tcagtttttg ctccccaatc agcatgcccc
    26101 ccttcccctg caaaagcact atagtgatgt tgatgaggaa ttaaataagc aacacccaaa
    26161 ccggttcctg cgcctaacac cactctcact ttactggcat ccaggtaacc attaccaatc
    26221 tgtaccagat cctgagtcga taatacaggt aaactcatcg caatcgcagt aaaatcattc
    26281 atgacttcca gaacttcaag gccaagtctc tgttttaact ctgttattga aaattgccaa
    26341 tgacaattcg tcatagaaac caaatcatcg atgactggac acgcaatcgc tatcgcaata
    26401 tgttttattt cttgtaaagc atgccggttt ttataagtta atagcgcgga ctcaagacta
    26461 ataaactcgg cacaaggaga aatttcaatt ttatccatct gcaaattgac aagattaact
    26521 ctactgaaac gagcaaaagt accgcctatg tccgcaacaa tagcatactg cgctaaatca
    26581 tgaggcatca taaaaaaatt catttccttt aaatagacta catgccccct gttctgcacc
    26641 agtaaaatgt aatcttaaat taccaaacaa ctctcgtccc atgcctagaa cagaggaata
    26701 cccttcgata gcaactggag agcggcctgc taactcttca tcagaaacta ataattgaag
    26761 tattcctctt tcacaatcga tcaaaatcat atcgcccgtt tcaattttag caatcccgcc
    26821 cccatcaacc gcttccgggg taacgtgaat tgcagcggga accttgcccg atgcgccaga
    26881 cattcgacca tcagtaacca atgccacatg aaacccttta tccattaaca caccaagttt
    26941 tggtgttaat tgatgtaatt ctggcattcc acatgctttg gggccttgaa aacgaactac
    27001 caccacacaa tttttattaa gcaaaccttg attgaataga tgttcaaatt cctcttggct
    27061 tgagcaaacg actgcgggag cttctatcac ataatgctca tcataaagac tggatgtctt
    27121 aataattgct ctgccaagat tgccacttaa aacctgcaaa ccaccctgag gtttaaatgg
    27181 atcggcagca gaggttaata cttcattatt gcttgagctg tcaggaccat taacccaaac
    27241 cactcgcttg tcaatcaatt gcggctgctg cgtatattta tccagtccaa aacccacgac
    27301 tgtgttgaca tcaggatgaa gtaaatcggc ctctagcaag gttttaacaa aataagccat
    27361 gcctccagct ctttgaaaat gattgatgtc tgcttgacca ttaggataaa ttcgagcaat
    27421 taatggaata attgccgaca attgtgcaaa atcatcccaa tttacccaat accctgccat
    27481 agccgctatc gcgacaaggt gcatggtatg attggtcgat ccccctgaag ccaataatcc
    27541 gataataccg tttacaatat ttttcacatc aagcatctgc cctattggca tataatcaga
    27601 atttaaatct gttaatgaca aaatctgaca tgctgctgct ttggttaatt cattgcgcaa
    27661 cggggtatta ggattcacga aggaagagcc tgggagctgc agccccatca gttcaataac
    27721 taattgattt gaattggccg taccataaaa tgtgcatgtt ccagccgtat gataggaggc
    27781 tgcttccaca tccaataagg catttttgtc gacttgacct gctgcgtaca attgacgaat
    27841 tcttgctttt tcctgattgg agataccaga aggcattggc cccgcgggta caaaaataaa
    27901 aggcagatgg ccaaatgcca gcgcagccat taataatcca ggtactattt tatcgcaaat
    27961 acctaagagg agccctccat caaacatgtt atgtgacaaa gcaacagcag tcgataaagc
    28021 gatcacatcc cggctaagta aacttaattc catccccggt tgtccctgag taatcccatc
    28081 gcacatagct gggacgcctc cggcaaattg cgcaactcct cctgcagcag agatcgcttc
    28141 cttaatcaaa tcagggaaaa cagcatacgg ttgatgcgct gataacatat cgttataagc
    28201 ggaaacaatg gcaatattgg cttttgcatt acctctcaaa atagccttgt cctgtttagc
    28261 acaagcagcg aagccatgtg ccaaattccc acaatgcaga atacttcgtt gtgggccttt
    28321 agtacgagcc ttatcgattt gattcagata ggccgctctt ctagcagcgc tacgattttg
    28381 gatattttct gtcacttgat ttattacagg atgcattctt tttcctcatc gcttggagca
    28441 tacatcacct gaacattggc atccggggca tttaaaatag cgcagattgg caggatctcc
    28501 gggttgttag ttgacattgc ctgctccaga ataacccgtt ttctttgacc tactaaatgt
    28561 aaaaaaatag tactgctgtt taataatcgc cttttcgata agctaaccct ctggaatgaa
    28621 gctgttctag gcttcacaat aagcaccgca tttgctttat catctaatcc tgcacttaac
    28681 tcatcactgc atggaaataa agaagcagta tgtccgtctt cacccattcc taaaactact
    28741 gcatcaaaca ttggtagaga atcaatttgg ctattcacct cattcaaatg atctaatgga
    28801 tcctcttttt catcaaacaa gctgataaat ttggcttttt ttgccttctc ttgcagcaaa
    28861 aattcacgca ccagcagctc gttacgatgt ggatcaaaaa ggttgacaca acgttcatcg
    28921 gccaaagtga tcactacttt ttcccaaggc aagtcggcta aagccaattc cttgaacaaa
    28981 tcgatagggg ttctaccgcc ggaaaccacc aaaaaagctt gccctcttcg tttgattgca
    29041 tctgatagaa tagttcttaa tttttccacc atgtcatgaa tcaactgttt ggcatcccta
    29101 aaactatgaa actgcattgt gatcacccca aacatgtgct tgcccattaa ataagcgtat
    29161 gacttccttt ggcccccatg tacaagctgg ataggtatac agcggaatat tatcttcttc
    29221 ccatgccgat acaatactgt caatccattg ccaagcctgt tcaatttctt ctcggcttac
    29281 aaaaagatac tgattaccta acatgacctc tagcaataat ctctcatagg catcagcaat
    29341 cctttgcgtt ttaaaactat gatgaaaatt taaatccaac ttggaatcat gcaacatcat
    29401 acgctcacca agacccggga ttttattcat tatcctcacc tccacccctt catcgggttg
    29461 taagcgaata attaactgat tagggtacaa ttcttcattt aagggatgga agatattata
    29521 aggctgtggt ttaaaataaa taaccacttc gctttgcttt ttgggtaatc ttttgccagt
    29581 aagcatatag aatggaacac cagaccatcg ccaattgtct acataggctt tgatagcaac
    29641 aaaagtttct gttgtagatg cctgattagc acccacctct tccaaataac caggtatttt
    29701 tttaccattt gctaaagtat tggccacata ttgggctcgt actgtatgat catgaacatt
    29761 agacttgtta ataaaacgca aagatttcag tacttttaat ttttcgcttc tgatgctttc
    29821 tgctgaaagg cttaaggggg gttccatcgc aactaaagat aatatttgta acaaatgatt
    29881 ttgcagcatg tccctgactt ggcccgactt atcataatat tcccaacggc cttcaacacc
    29941 gatttcttca gcaacagaaa tttcaactcg atcaataacg cgattatccc aattggttga
    30001 aaatatcgaa ttagcaaagc gaagcactaa taaattcaag acggtttctt tacccaggta
    30061 atgatcaatt cgataaatct gatcttcagc aaaaaaacaa gccacttgtt cattggttgc
    30121 tacagaagaa ggcaaatcat ggccaattgg tttttccaaa acaactcgcg atggtttctg
    30181 tgttaaatta aggcgagcaa gactgcggca agtttgagaa taaaggcttg gaggaattgc
    30241 caagtaagaa ataaccactc gctcagaagg gttaacatag tccgttaatt tttgataatc
    30301 atctgattga tttagatcaa gtttgcaata agccagtttc tttacaaaac gtaaccatac
    30361 tgcttcatca atttcatcaa ataaaaattg ctgaatttta tttttaatca agtcaacata
    30421 agcagaaaaa tcgagctctt ctcgggcaca cccaataatt cttgtttttt cgtgaagtaa
    30481 attagccagt tctagttgat ataatgcggg caacaatttt cgacaagaca agtcacccag
    30541 cgtaccaaac aaaattaaat cacaaggata cttcgattct gcttttaaga gcatatatca
    30601 tcccaaaatt acaataacta tgtccctgaa attgcatcga ttttacatca ctggaatcaa
    30661 gcgtcaatca aaattgattt ttttcgctca ttcgatcagg tacattagct gtttaatcat
    30721 tcaaatcaat tgaagtgaat taaaagcggt agaggatgaa aaaggattaa aaaatctatt
    30781 tggaagctac ttttatgaac tgcaacatta tcaaaattag cgatttttgt ctaaaaattg
    30841 caaaacctca tttttatgct atatgaaata agtacacgtt agtaaaaaat ccttttgatt
    30901 aggagccact catgcttaac aagagatttt cagaacgact gaataaagaa ctggataata
    30961 ttggagtccc tgaggccaca acagagcgaa ttgagatact ttccaaactc atcaaaattc
    31021 caaaatttaa agcagaagct ttgcttaatg gaattacgag ccctgatgaa gccgtattaa
    31081 aaacattagc tcaggaactg gaagtcaatg cggattggct aattggcaaa agtgactcca
    31141 aatcaggagc ccattagaaa ttatccatga aatctatgat tggaactaat tggctttgct
    31201 tttttctcaa tgtattcaat gatttttcct gccaaattaa tattagttgc tttttctata
    31261 ccttcaagac ctggagaaga gttaatttct agcactaatg gaccatgatt tgagcgaatc
    31321 agatccactc cggcaactct taatcccatt gtttttgcgg cactaacagc aattgcccgt
    31381 tcttgcgggg aaagtttgac ttttactgct tttcctcctt gatgaacatt tgcgcgaaac
    31441 tccccatcct tggcctgccg tttaactgcg gcaactactt tttctcctat gacaaaacaa
    31501 cgtatatccg tacctcggga ttcttcaatg aattcctgga ccaaaatatt ggcatgcatt
    31561 tctttaaaag cattgatgat actcacagct gattgatggc tgtcagcaag aatgactcct
    31621 ttgccttgtg ttccttctaa taattttatg accaggggag ccccaccaac catatgaatc
    31681 aaatcttcag tatcatcagg agactgggca aaactggtta acggcatagg aattccttta
    31741 cgtgccagca attgcaaaga gcgaaattta tccctggaac gtgatatagc tatggattca
    31801 ttcagagtgt acatgcccat tgtttccata tgacgcaata ctgccgtacc ataataggta
    31861 atggatgcgc caattctagg aataacggca tcaaaatgcg gtaaaggtgc cccacctcga
    31921 taatgtacct taggattaga agcagccacg ttcatataac aatataaagg gttaataatt
    31981 ttaacctcat gccccgcagc ctcagcctca gcctttaaac gcttatgaga atacaaatgg
    32041 gggttagttg ccaatatcgc aattttcatg aattaaactt tcctcagtaa tttcatactt
    32101 caatctgaac aatccaaacc attttcccta attttctatt aaataacgca agttttaagc
    32161 atcacaacag agctgctctg aaatgacaaa agcagccaag catagaaaat cgcgggtttt
    32221 aagccaagat aaatgaaatg tcaacaatcc ttacttaaag cctagattaa aaatcacaaa
    32281 ttatcctccc tgccataccc aacagagagt aaatttttga attcatgcaa cagtcaatta
    32341 attaactaat gcattctaga tagatttctt tgacataaat aaaacaagtc tgcttagcta
    32401 ttgctcctgt agagataaaa tatcgaatta caggttaaga taccaataag atataatcct
    32461 tagctaagag atccgcatga tgtggtgttg taaaaaatgc atttaactca ccttgaggat
    32521 taaatagcat tacggcccca ctatgttgta tatcataatt ttcaggctcc gaactctctt
    32581 tattgtttat ttttgcgtaa gcaatgccca tttcatgggt cattgattta attgaatcct
    32641 cttctcctct tgcaccataa aatgatttat ggaaagaagt gacataactg cccaatttat
    32701 caagactgtc tctttgcgga tcaatagata tcatcacaac ttgaggtaaa tttttcaccc
    32761 ctttcttttc cagaattcga tacatttttc caagttctgc catggtagtc gggcatagat
    32821 aaccacaatt ggtaaatcca aaaaatacta aagtccatcg accttgcaga cttgagttat
    32881 caaaaacctt ctgatctgtg cctgttaagc taaaattatt gactgttctt ggattttcaa
    32941 gataagtccc gtgaaaatcc gccacattga tcttcttttt aaaatgaaaa ttctgagaga
    33001 tataaatacc agccaataaa ctgaccaagg ccaatacagt gattgttaca gcgccccctt
    33061 ttatctttat gttcattcta accccttaaa aatagtgatc tactaataaa aagacaaaca
    33121 acagcattag gtacagaata gaaaatcgga atgtttgcat agcaacaact ggtttatcag
    33181 tgaaatacaa tttatgtgcc caaaccaaaa acctgatgcc caaaaccaaa gcacctagca
    33241 aataaatcca gccgctcata cttacaacaa aaggcaaaag actgactacc agcaataaaa
    33301 ccgtatagag ataaatattt aatttagtga attgaatacc gtgtgtcaca ggtaacatag
    33361 gaatttcagc atcttgatat tctttatatc gataaattgc caaagcccaa aaatgaggcg
    33421 gtgtccatgt aaatataatc aatactaata acaaggcctg aggatccagt tggtcagtaa
    33481 ccgcagtcca acccaataag gggggcgcag ctccggctag gcccccgata acaatattct
    33541 gcgaagtagc tcgttttaaa taacctgtgt aaacaccagc ataaccaatc aatgttacaa
    33601 aggtaagtaa tgctgttaat tggttcacaa acaggataag aagacttaat cccattagcc
    33661 cgataatgac cgcgaaccat aaggcttgct ttactgacac acgtccataa gcgactggtc
    33721 tcttttttgt tctggccata atcgaatcaa tatgcctgtc taccaaatga ttgattgcag
    33781 cagcgctgcc agcgcacagt cctataccaa gtaaagtaaa agcgattaac cgcaagctta
    33841 cccagccagg agcagctaaa tacattccta caatgacagt gagaagcatg agcaacacga
    33901 cgcgtggctt acacaattcc acgtaatcac gccaatccac aggaagttga gctgcgtatt
    33961 cagtacgcat ggctaacctc cctatcttgt gcaaaatgca atgaacaaaa tgccaccgct
    34021 aataataatg cggctacacc attatgagcg accgcaacct tgatcggtaa tagatgaatc
    34081 acattcatta tccctaaagt gaattgaagc aatactaaca tagccatgat aatagcagtt
    34141 gcttttaaat aaattgaatg cgttttgaat acgattatta atgacagaac cagtagataa
    34201 gtggcagtca atatagcccc tattctatga ataaattgaa tgctggccct aacttcactc
    34261 tcaagaacac cgccttggta attggcgcct atcgtagaaa ataaattaaa tccttttgca
    34321 aaatgcaatt caggaaacca gactccgtta cataatggaa aaccaataca cgaaatacct
    34381 gcataattgg cacttaccca accacccaaa gcaatttgca ataaaatgat aacaattccc
    34441 aaacgcaacc aaggtcgcca ttcaggtaaa ttttgcccct ttagctgggc tatctgtaaa
    34501 cgaaaacggc ataaacaagc aacgattaac atacctccca gcaaatgtcc cataacaact
    34561 actgggagca attttaaagt aacagtccac attcccaatg ccgcctgaaa taccaccagc
    34621 ataaataaag ccatgggaag atgccaaggc aaatcttgat cctgacgacg cttgaaccat
    34681 acctggaaag cgatgaaaaa aatcaacagg gctaacgtac ctgccgcata gcgatgagcc
    34741 atttcagtcc aggctttctt ggattcaata ggaaggtccg gatattgtga ttgagcttct
    34801 tgcaaacctt tatctgctcc tggaagaacc atgcggccat aacaacctgg ccaatccggg
    34861 cagcctaatc ccgcatcagt taaacgagta taagcgccta acataacgac aaaaaaagcc
    34921 aaaaaaacag cgagagtcgc tgctaatcgt attgaactac tagacatcaa tttatccgct
    34981 cttaatttca gttgtattga gtaaaagttt caaatcttta tatatatcgc ctgggttaca
    35041 tcctggttga tagctcagta tgaggtaatt atccgggttt gctataaaaa ttttagcttc
    35101 aggatataaa gtcgacaatt tagacatttc agttaatgat aaggcagcaa catggatatc
    35161 ttgctctttt aaaatgggtt ttaattcctc tgcgatcaat gaatctttgt tgcttagaag
    35221 caaccattca tccacctgat acaattttct cccaaaagaa aggcggactc ttcccaacat
    35281 attaagctgc ttaagacaag tcttgtcgca gccattagga ttccaataga taatgcgcca
    35341 ttttgcactg ctatctaacg gtttgagagc gatcggtgga gaaagtaaaa ttcctttatt
    35401 tactttagag gttccaagcc aggaaggatg tttatagaac aaataagcgg ttattcctgg
    35461 tgcagcaaaa atcactacca agagtaataa tgtcatgtat ttatatggtt ttttcatttt
    35521 tcttcaaatt aaaaatcacg aatataatca gaataaccaa tgccatggca aaccactgta
    35581 ttgcgtaagc aaagtgcctt tgcgggggca tggatactat ctcccattca cgtacaaaac
    35641 caaaaggctc attcttgtcg aggcgaataa taaacggata gacctttttt tgcaaaagtt
    35701 gtttcagtat ttcttcatca atgagctcaa gaagagtcac cttattctct ttttcttcat
    35761 aacttggccc taatacccat tgttttttac ttggaaaata aaccatgcca aatagtttaa
    35821 atttgccgtt gggagtctga acatcaggaa aagtgcgtcg agtaatgtct ccagaaaccc
    35881 atcctctatc caccataacg atggagccat catcaagaag cataggagaa accacgtcat
    35941 aaccaaactg atgttgataa tgttgattat caagcaaaaa taactttggc agaaaaactc
    36001 cttcaatgct gattcgttga tattgctctg gtaatttttg ccctggctgc caaattatgg
    36061 gctcctgctt tgccagcgct tgttgtgcgc ttatcatttc agttttttca tcggctctgt
    36121 gtatttgcca aaatccaagg ctaatgaata aaaaaaagca aacggccgta agaataagca
    36181 ttggccaatt tgaggtgaaa cgaaaattaa aacaggttaa actaggcatc ttttatcaac
    36241 tactcatttg gttagctaac attcccctga agctagcaat gtcaggagta taacaaatgt
    36301 tcactaaaat cttcatcatc tttgtcatga ttcttattgt ggcctcatta gcgagtgggc
    36361 tcttttacct ggtcaaggac actgataaat caaagcgcac agtcaaagcg ctcaccgtgc
    36421 gcattgcaat ttcattatct ttatttgtgt ttttatttat tgccttcaaa ttgggattga
    36481 ttcaaccaca tggaatttag cttttggctt ttaataaatt cacctcaaga gatagggtca
    36541 tggctgagat aatgagatat tttttaaata gtcatttcag ccaccttcag gaaattcccc
    36601 ctaatccgcg agacgacaaa caacgagaaa aaatctcccg gtgagcatct attgaccgta
    36661 aaccaatacc actgttccag gtaggggcgg ctactctctt acaataaaat ttcgaaattg
    36721 ctcaatatac tatctatact tttgttgtaa ccctcaaaag ggatggtgag catcatgatg
    36781 atagttctgt ttttatttta tttcataata ggattacaaa ttatatttaa acccaataaa
    36841 acgataatac tccaatttct tttcaatttg cttcttatac taatcagctt caattatcac
    36901 agccatctgg tgaacctatg aataaacaaa tgcaatggca tcttttcggg aatacttcgg
    36961 ggttattgat aatttgttgc cttttaatct tggccttcct ggatcaattc atcttttatg
    37021 aactaccctg cccaatatgc attttgcaaa gaatttgctt tgttgcagta ggtctctgtt
    37081 ttgttatgaa tttaatgctg ggtattagag cttcacatta cgggctgatg cttttggcaa
    37141 gtcttttagg gctgtctatc tcggttcggc aaatgtatat tcatttaaca cctggtgatc
    37201 ctggttatgg cggcctcttt ttgggtttgc atctttacac ttggtcagca atcatatttt
    37261 tagtcattat cttattaatt gctggggctc taatccttga taagggcctc atgcccgatt
    37321 ataaagtacg aagcaagtca gcaaaagtaa tgatatgggt attttttatc ttgattcttg
    37381 cgaatggaat cagtacattc ttggaatgtg gaccgcacga atgtccagat aacccgagca
    37441 aatattattt actaaataat aattaaggat cttgaggatt taaaatgcgt cttttgttta
    37501 tcagtttaat tcttgccagc ggatatttac aggccgatac aataaataat tatatgaata
    37561 ttgctaacaa tattccgcaa atggaaatga aagcagaccc tcaagctcaa gcatgggcca
    37621 gatcggcgcg tcatgtactt accatcacca gtgaaagtat tgctgaaact ttaatccagg
    37681 cgaatgaagc tgcaaaaaca caggggaaac ctatcttttg cttaccacaa ggtgtgcaac
    37741 ttaattcttt tgtaatgaac gagctcattc aacaaaccta taaagaaata tccagtcaac
    37801 aaagtgacaa agataaaatg accgtttctc aagtggcttt attagggctt agcaaaaagt
    37861 acccttgtga acccagtcct caggaaaaac aaattcaaca tgtggcttcc ctgttatcgc
    37921 cacaatagcg cccgcagcaa caaagttccg gaattttttt ctacatccgc ttctttaaac
    37981 cagacgacat attctgaaaa ttgttagagt gggcatgaca aagcaaagtt ggcagagcgt
    38041 caaacactgt gaattccctt gccaatgttt gcaaaaactc tgccttgctg gtttttttat
    38101 tgattttagt gtttattaag cgcgacttta gcttcttgat aattacttac taaattcctc
    38161 aaaagcttcc aatgcctctg cagcatacat taaagaaggg ccgcctccca tataaaccgc
    38221 catacccaat gtttccagta actcttctct tgtcgcttgc agtttcacta aagtttgtga
    38281 atgaaaccct atgcatccgg ggcattgctt ggccacggcc aacgccatag cgattaattc
    38341 cttggttttt ttatccagtg cgccgtcttt cgtcgcagct tgtgctaaag aagaaaatcc
    38401 agtcattaac tctggcatct ctttgcgaaa cttggctaat tgcgtagtga tatcttttgt
    38461 aatgtgtgaa aatttgtctg acatgaggct ctactccttt ccttattata ttttttcaag
    38521 cgtctctaat ttacagcata atattgggat gcaaaagatg tattgcctaa tttaacgtga
    38581 gtaatgtata agataggtcg ttatcttgaa ttgtccaggc tgaggatatc acctgattct
    38641 tatacaatcc caatcgataa ctcacattaa tttaagaagt gtcgatttca ggatattgct
    38701 cgattgagcg tttctttagc cagggtaagt cttttttcaa cattccctaa ttcagtttgt
    38761 aaatgcaaaa tagttgcagg actagagcag gacttcattt gctctaaagt ttttctcatg
    38821 gcttgatatt gttcctccag ggcgcagatc ttctcttcca tggttttaat ccattgttgt
    38881 cttgtactaa gagttgttaa atcaaaagaa atttttgatt caggtttatt attctttttg
    38941 tccatttgac ataatacgac caagacatta atttttttct gaatacgctc tgccaaaaaa
    39001 catgcaattg actcatcctt atgtttctgt aaagcgtgaa tatcatcctg gatttccttg
    39061 atacaggcag gtccattgaa ttctataacg gaagaaaata tacctttagg caaattatga
    39121 attgaaatag aagaactcaa tccattgatc ttccattcca gctctggcaa tcgtgcagat
    39181 agttcttcca acaattcgtc aatataggcc atcttttgaa agttacataa acaactgggg
    39241 ttattaaact taacctgtct tgttaaatta tacagcaggt tccaaatccg cctctttggc
    39301 ttgcaaccaa ctggttataa aaggaggagc acacatgagc agcaagccaa taatcaaggt
    39361 caattcatag cgtgttgcac tgccgacatt aatcccctct ggaggaataa agctaacagc
    39421 taaagtggta accactccaa tgatgcctat gcctgcaaca aacaacattc ctgccatacc
    39481 tcctggaatt ttaaaaggtc tgggatggta aggttggctt atgcgcaatt taattgctgc
    39541 gacaaacatg atcaaataca tcagcatgta taattgagcc gccaatgctg ttaagagcca
    39601 ataggatcca ttgacactgg gcataaataa gaatagcgcc gataatactg tgactatggt
    39661 tgcctgggtg tatagcatca tggccggagc accttttcca ttagtccgtt gaaatacctc
    39721 tggtaaattc ccttcttctg cggcaactaa taaacctttg gtaggggcaa taatccaatt
    39781 actcacacca ccaagccctc cgagaaccaa catcacagct accacaggca tcatccaatc
    39841 catgtgatat ttaaggaaaa aggcagaaaa ggcttgcata attccggcaa ccagattgat
    39901 atccttttgt gggagtacta ccgcaatcgc taatgaacct aaaatcaaag tactcaaaat
    39961 gatccctaca gaataaacca gggctttggg gaaagcatgc tggggatcct tcacatcatt
    40021 agcatgaacc gtagcaatct ctattccgca aaatgacaat atgatggcgg ttaatgaaac
    40081 ccacatgccc ttatccgata aatggggaac aatactgggc atgtcaaaac tgatttgcaa
    40141 ggggttacct tgagctatcc aaacagcacc caaaccaatg atcaaagaca tgggtaataa
    40201 taatcctgcc aaagaacaaa tattactgaa catggccgat gatttcatac cgcgcaaatt
    40261 cacaatggtt gctccccaaa agcaactgac aatgactgcc caaagaaaat aaggattggt
    40321 agttaaagaa gggttaataa gataacctat tgttcccgca acaaacgata gaatcgtggg
    40381 ataccaaatc acattttcta tccattgcaa ccaaatggct aaaaaccctg attttttccc
    40441 aaaagcttcc ttaacccaga tataaacgcc accctgcctg gcccacccag aagctaactc
    40501 tgccgaaacc aatgcggttg ggataaggaa gaataaggca cccaggataa aataaaaaat
    40561 caattgactc ccaaataatg cagtagctgg caagttacgg atactgtcca cagagccaac
    40621 ggtaatcata gtcaagctaa atatagtgag agaatgtttt ttattgctca ttgtattcct
    40681 tcagtgagga ctatttggaa tcaaaagttt attataaaga aataagctta aggaaacctt
    40741 aataatcgaa taaaaaataa ccaaatatac cccttagtat actgacctac aaaattgacc
    40801 caacaggcat tgagaatttt catttcagta caggtccgat gcgaaaacat catatgatgt
    40861 tatgtcatta aaaaccagta ttctgtatat aaaattaata ataagaatgt ttctaatgca
    40921 cattaagtgg tttgtttact aataggatgc aaaaccccaa aatcgagaca aaaagcttca
    40981 ggtgctaaaa agacaaattt tattctccta tttcatacca aaaccaggtt gcggtgatgg
    41041 ataagaaata gttttcactc ctctttggaa caaagtacct tttgtctcag aaaatttaaa
    41101 tcgacgagag gttgagttct cctgcgcaca agctcctttt tccaatctct cctcgatcat
    41161 ttggattgca agggtatgct catttttaag agcatgatca agtgcggtca aaccgtcatt
    41221 atctttaata gaaagagaag cgccttttgc aaacaacaaa ttaaataatt ttctatcctc
    41281 cgtcactaca gattttttac aggcgtacat gagcggcgtt ctccccttgc tgtcttgggg
    41341 atcgatttta attccacgta tgttcaacag atcttcgact ataccactac caccataccg
    41401 acaggcaatg tggagcaaag tagaagaatc gcttttacgc ttttggttaa ctctatgtcg
    41461 agaagtactc agcatggcac gtactacgtc tcttcttcct aaatagatgg ctgtaaggat
    41521 aggcgaatca tcacttagcc gttgtcgaga aaaaattgac cagcctgaat gaatacactt
    41581 ttttacagaa ttcatatcac cctgagtagc tgccatggaa aaaaaagcca atcgttcggg
    41641 ataagaagtt ttggttctga ctggaaacac agggaataaa ttctgcagtg cacttgacag
    41701 tttttcttcc ggatgaatgg aaacaaaatc ggtattcaca gtcaatcgac ttccctgaag
    41761 gttctcggca cacacacgat acaactcttt aaccaggttt cgggacgaga actcaaagta
    41821 aggtttattt ttgtcttgtt ggtataagtc attgatatta ataaaccgcc agatattacc
    41881 agccagttta aatcccatgg cgtgatctgc ggtactgata attactgcaa catcctctct
    41941 gatgccagtt ctttttaata attcaagata ctctgttgct tcatcctgat ttaaaaaact
    42001 atgcgtctta taaaaaatgt gctttgcaac cgaagagtcg gcatttatgg ctttcaaaat
    42061 ggaattaata tcaggctgcc ataccagctt tttgtatttc tctttatatt gtaagggatc
    42121 ttgagcaata cagattttct ttcctaactg aggcaaagtt tcaatgatct tctcatcttt
    42181 gcttaatgat tggttccttt ctttcttttc tttaatttgt tgtaatgtct caggcaaacc
    42241 aagctgttgg gttcgtaaat gatgcaattg gcggtagaaa aaggattctt taccctgagc
    42301 cactgctaat gcccaattga gagtgaagcc ataacagact ccttcaaacc tgggatgacc
    42361 taattgtgtt aagacattcg taataccatc atgtgttaat tcactggtgt cctgtttttt
    42421 agagaagaaa gaagtagaat acggcaggag cttgggcatg agttgataca caaccaaacc
    42481 gcaaactagc ataatacaaa ttaaaacata aatcatatcc cttgaacctg atgctatcca
    42541 taatgacttg agtataaaat taaaccatca tacaagtcga ggggatgaga tattcgatta
    42601 gcatcaagag cctccaaaca agtcaggctt actgctctat tatcccccta ttgcaccgca
    42661 tgatacctct ctgatgcccc ataatgaaaa catttaattc ttcttaagga aaccttaatg
    42721 ttaaggcttt aaactatggg tccaaaatat agacaattac tttttttaaa aaatcattgg
    42781 agtcccttat gcctaaagca tttacccatt tgtgtaaaaa agtcaatctc agtttatata
    42841 acgatacatt gactgtctac cctaaatcat ctgtactgcc aaaacctatt caaatacgta
    42901 gcgaacttga tcttcttatg gttctgttgt tttctcaaat ggattgcaac agcgcacagg
    42961 ttgaccggtt ttttatgatg cgttcgaatg aactcggtgt ctaccgtttt attcatcgtg
    43021 acgacactga tcatgatttg tatcatcaag atgaatttag catggatgag aaccattacc
    43081 atgcacaatt tgacaagact attgacgaga gtaaattgga aaacattctg ggaatactgg
    43141 aaaaacattc tcttatttcg tctgaagaac acgtaagctt tatcgaagcc taccataaag
    43201 ccaatactct acccgaagat atgccaaaac tggaaacaac tctgcccagt cctttacctg
    43261 tatcctctcc ttcagttcac tcaggcacaa aacgacctgt tgtgaaagaa aaagaagtag
    43321 atactccaac cactttcgca gggctaagag taggattttt ccttacctca acaaaaacaa
    43381 caacaaaacc cttatccaga tctcatgatg atgagaagaa aaccgggttg gataggcatg
    43441 agttccatta atcgatatag tttgataaac catagtaaag atcgctctat tggtttactt
    43501 aatatgacaa tttatggtga tttacccaaa atctaaaaaa ctgtagagta actaataaat
    43561 tactctacaa cttcaaatta caaaattata aagataaccc taattgattc actgttatca
    43621 attactgtga ctcattaata cgtttttctc tgtggtatac ttttaactcc ctatgttata
    43681 gtagttattt tttaggtact acttattatg tttgaaaatt taaccgaacg cttaacccgg
    43741 acctttaaaa atttgagggg tcaaggccgc ctgacagaag acaatatcca gcatgctttg
    43801 cgtgaagtaa gactgtctct tattgaagca gacgtggctt tgcctgttat caaagagttt
    43861 attgagcaag taaagcaaaa gtctcttggg caggaagtct taaccagttt aaatcccgat
    43921 caggcattca ttaaaatcgt gcatgatgaa ctcattcacg tcatgggcga tgagcgggtt
    43981 gaacttgatt acaaaaccca accgcctgct gtctttttaa tggccggctt acaaggttct
    44041 ggtaaaacaa cgagtacagc aaaactcgcg cgttatttga aagaaaccga aaataaaaaa
    44101 gtcatgatgg caagcgttga cgtatacaga ccagccgcca tacaccaact gaaagtatta
    44161 gccgaacaaa tagacgtcgc cttctttgat tcagacccta atgagcaacc gataaaaata
    44221 gctcaaaagg cattggaaag cgccagaaaa cagtatgttg acgttctcat tctcgataca
    44281 gcagggcgcc tgcacgttga tgaagacatg atgtctgaaa tcaagtcttt gcataaagca
    44341 gtcaatccaa tcgaaaccct ttttgtcgtt gacagcatga caggacaaga cgctgccaat
    44401 actgcaaaag catttcatga agccctccct ctgactggtg ttattttgac caaaacggac
    44461 ggcgatgctc gaggaggagc ggcgctatct gtcaaacaaa tcacagggaa acctataaaa
    44521 tttataggga gtggtgaaaa aatagaagcc ttggaaccct ttcaccctga acggattgca
    44581 tcaagaatat tggggatggg agatatttta actctcattg aagaggtaga gcgcaaagcc
    44641 gacaaacaag ccagcgaaaa actggccaaa aaattaaaaa aagggaaagg gtttgactta
    44701 gaggatttca aacaacaatt attgcaaatg aacaatatgg ggggtatctc cgggatgatg
    44761 agcaaattac ccggagtggg gcaattgcca aagcaagcca tgcagcaagt gaatgacaag
    44821 gcgatggctc aaaccattgc aatcattaac tctatgactc ccaaagaacg ccgtataccc
    44881 aaaattattg taggctcacg taaaaaacgc atagccttag gctctggaac tcaaattcag
    44941 gatgtcaaca ggctattaaa acaatttgag cagatgcaaa agatgatgaa aaaatttacc
    45001 aaaccaggtg gaatgcagaa aatgatgaga ggaattggtg gcatggccgg attgaaaggc
    45061 atgtttccag atgatttaaa ataattcgta agaattactt cacatgtcga tgtaattttc
    45121 gtacaataca cggcattttt atgtaaccac tctaacagta gaggaaaata atggtcgtta
    45181 tacgtttatc acgagctggc gctaaaaagc gtccttttta tcacatggtt gttactgaca
    45241 gccgcaaacg tcgcgatgga aactacattg agcgtattgg ttattttaac cctgtagcac
    45301 gtggacaaga agtaaagctt cacattgata tggataaaat gacccactgg caaaaagttg
    45361 gggctcagtt atccgatcga gtcagtgcct tgttaaaaga acatagcaaa aaatcagaga
    45421 cagctgctta accaaagtga ataacaagac aaattgggtt atcatcggcc gttttggccg
    45481 accccatggt attaaaggct ttgtcacggt acattccttc acagaccctg ctgataatat
    45541 cttgaggtac aatgactggc acgttttttt aaacaaacag tggcagcccg ttaagttatt
    45601 gacaatagaa gtaagaagta aagcgattat tgctcaaatt gaagggtacc cggagcgtga
    45661 attagtctct gcattaacca atttagatat tggtgtgcaa gaatcacagc ttgcagcttt
    45721 ggcaccaggt gagtattact ggtatcaact gattggcatg agtgtcataa actccaaagg
    45781 cgatcttttt ggaaaagttg ttgaaatcat gcctactggc tcgaatgatg tacttgttgt
    45841 ggaaggtgaa aaaagacatt taattcccta tcttccagga caatttgtaa tcaatattga
    45901 tgaaagccag caaataatca ctgttgattg ggatatgaat ttttagtggc attacatctt
    45961 ggagtcatca ccctgttgcc tgaaataata caaggcatcc attatggggt aaccggccga
    46021 gctattgaac aaggcttggt taaaattgat tgctggaatc ctagggattg gtcatcaaga
    46081 ccttataagc aagtggatga caagccctat ggtggtggac caggaatggt catgatgtat
    46141 gagccattgc atgctgcaat aaagcatgcg cgcagtgaaa tgaaggaaaa ctgcaaaaca
    46201 atttacttaa gccctcaagg caaagtagtt agacagaacg atttaaaaca aatagcagcc
    46261 aaaaaacaat ccttgctatt tgttgcaggg agatatgaag gaattgatga gcgaatcatt
    46321 agtcaccacg tagatgaaga atggtcgcta ggagattttg tattaagtgg cggtgaattg
    46381 gccgcaatgg tatttattga tgcgataatt cgcttgatac ctggaagcct tgggcatttg
    46441 ggttcagcag aacaagattc atttatgaat ggattactgg actgcccaca ttatactcgg
    46501 ccagcaacta taaatggact ggatgtacct gatgttttat taggaggaaa ccataaagaa
    46561 atagaacgct ggcgtaggaa acaatccctg ggtaaaacct ggctcaaacg cccggattta
    46621 cttgagaaag tacaattaag tgaaacagac aagcaattgc ttgctgagtt taaatgcgaa
    46681 cacggcgatt cctgctgagg atgagccaat ttgaggagta gtctatgact aatattattg
    46741 accaaattaa tgccgaacaa atgcaaggca aagaaattcc tgatttcaac ccaggcgata
    46801 ctgttctggt ccaagtaaaa gttattgaag gtaaccgtga gcgtttacag gcatttgaag
    46861 gcgttgtgat tgccaaacgt aaccgtgggc ttaactctgc tttcactgta cgtaaaattt
    46921 cccataacgt cggtgtagag cgcgttttcc aaacctacag tcctattgtt gatagtatta
    46981 cggtgaaaag acgtggtgac gttcgtcgtg ccaaattata ttacttaaga aatcttgccg
    47041 gtcgcgctgc tcgtattaaa gaaaaattat ctgggaagaa aggcgattaa ttcttgaatg
    47101 gtaaaatgct cctcatatct atttttaata ctcctgtggg taacctggaa gtagcccatg
    47161 atgagcattt tattttcaat gcgtccttca cagacaaatc tggcactcca gcccaaaacc
    47221 aattggccca tttaattgcc ggtgaattga aagcttattt cgacaatccc catcaccgtt
    47281 ttcagctccc tctgaaacct cagggaagcc tcttccagca aaaggtttgg aatacgttgt
    47341 tagtcattcc cgttggcaga acagttacct atggagaact cgctacaaaa ctgcaaagta
    47401 gtccaagagc agttggacaa gcctgtaaat gcaacccact ggctttattt gttccttgtc
    47461 atagagtagt agggaagcat gatcttggag gatacatggg aaatccaaac gccatttctt
    47521 ataaaaaaaa tctgctcgct catgaagcaa aaatcatttg atgaattaag tttataaaat
    47581 cgatattaca tagatcagaa cttacaaaag cacaaaggca aacaatattc ttaacgaaga
    47641 gattagcaag tcttgtgaga ttaaacttta ggattcttat ccgtatcaga attgatattc
    47701 aaaggtgggg aaggaggttg taatatactg aaaaatctgg cgcttaatcc agttaatatt
    47761 cgtacagtat tatcgataaa cggcagcggc gaaattttct ttaaaaattc ctcttcgaac
    47821 tcagcaggct gcatttccca tagctttttt acatacacgg ctatttcatc atggacatct
    47881 tgagttaatt cagtttgctc atggagatac ttggctagtc gaacaaccaa gctgatgcga
    47941 gaagatttta attgatcagt tggcaagttt gcagactcta ataaactaag ataatcttca
    48001 attaaatatt tcaaataggt tacagaataa atttgatcaa acgctttaga aaccgctgaa
    48061 gagtctgctt ccttaaccca agtaaaaaat ttatcttgct cttgccctaa aaattcgaca
    48121 aaccgttgct tctcaagata agcattgttc atagtcatta attggagcac ttccttactt
    48181 gataattttt ttgaagtaat ataatgctcc tgcagattat ttgttaaata caccatacag
    48241 ggaaaaagag cgatagaaat tgtagtgagt gcataggata atccctctct taaaggcctg
    48301 gactccattt catgaacggc atacaaaata gcaaattttt taatgtcatc caaacaaaag
    48361 ccattattta accattcaaa tatgagcaag ctcaccattt catcagcttc ttgatttttg
    48421 tcgctcttac cataatattc tgcaataaat tttggccact gtagaaatag aaggtatttt
    48481 ccatcacgta aagcttgaag atattccttt gttgatgaaa gcatagttat ccattaccaa
    48541 aatctgatta ttcaatttat aacattaaca atactaaaaa tcttgtgaaa aattatttca
    48601 tgttaaacaa catacgcaca tcaattgtta tcctatactt aaagtaaaac gacaaatcag
    48661 gcgggggatt ccatgaaaaa taaattagca gtgagtatat tattattcct gttaccaaca
    48721 actgcagcca tggctaattg tgatttaacc aagtttcgat gggattgtga tcttcctgta
    48781 caggttaagc ccagtccagg cgcttcctct ctagtgtatt gtggaagctc ttacggctat
    48841 atcacaaagc agcaatatga tattctggct cgctatcaac gcgccagtgt caatatggtt
    48901 ttggatatca atggtgaata cattgatagc ccgtgtatag gagccgagcg ttaaaattat
    48961 tgtagcaatg acttaaaata aacaatgaga aaaagaaaag gaatattatt atttccagaa
    49021 aatttcttat tcaaaatcag aagtctctgg ac
//
LOCUS       DBNCWK010000010        47358 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.010, whole genome shotgun sequence.
ACCESSION   DBNCWK010000010 DBNCWK010000000
VERSION     DBNCWK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 47358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47358
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.010"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            70..582
                     /locus_tag="AC06IO_000485"
     CDS             70..582
                     /locus_tag="AC06IO_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873701.1"
                     /translation="MSVINGKKIITIILGIACLFCSIHPVNSAPDFSNKPHVREKNKN
                     LLGVFVGGNHAQNDEAFTYGFEYHRVLSMPFGFSAVIEHTPVNVEQNNQVELIGLGTL
                     NVFRNLTFGIGPGIRYEKDEPNRMVGRLGIGYIIHFPPDIEITPNIDLDFIEGGEKEL
                     IFGITFGKQF"
     gene            717..1976
                     /gene="lem19"
                     /locus_tag="AC06IO_000486"
     CDS             717..1976
                     /gene="lem19"
                     /locus_tag="AC06IO_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem19"
                     /protein_id="HIO9873702.1"
                     /translation="MIDMISITTYWQQFKDCIDQLRSSVDNKEYSNQIATADLMIEIG
                     LTIEQLGITQIQQVGDRLHEKAKLFGYRVPFVTPEKKYEFCLPKITNAETLIFAIVYY
                     QHLKNYENVEYQGSINWFRSKVIHGVNEKKISTRAQSEAMLGEIRQYCQSQNIAFNES
                     EFIKKNDESAKEYTTRLFKNFHTDKTAKSEPIGLKQLNESKESNLSDELAKLEKSFED
                     SRNKRNQLIVKIDALAKKLADYHLLSQNHHKLNNEWNNKWFFTKFFDWIMSWFSNDSF
                     IKNLKTSYEQVINAEHELKQEFHPFDTADAYRAELKKQLEETHIECDKNQKQINEYQL
                     KQLEYKLEQRLEQKLQEKLQKDTAVEQKEPPVTKVLSEIKHETNGSDFSSNLNHYYGF
                     FKEHLPNRETLGAIAVGVAAIAIQNLM"
     gene            complement(2136..2822)
                     /locus_tag="AC06IO_000487"
     CDS             complement(2136..2822)
                     /locus_tag="AC06IO_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIO9873703.1"
                     /translation="MSKTERLFHLLQILRRYRYPLSGQFLANELGVSTRTIYRDIAVL
                     QAQGADIEGEPGLGYVLKPGFMLPPLMFNEEEIEALVLGSRWVAKKTDTHLQQAAHNA
                     LAKIASVLPADLRQQLELSSLLIGPGEVISGEDESLALVRKAIRKELKLFIQYHDLKG
                     NVSTRTIWPIGLGYFDQVRVLIAWCELKKDFRHFRADRIVDVQNLGVCYAKRRSVLLH
                     DWRKQNNIPG"
     gene            complement(2809..3582)
                     /locus_tag="AC06IO_000488"
     CDS             complement(2809..3582)
                     /locus_tag="AC06IO_000488"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9873704.1"
                     /translation="MNSRIETFQQMEWHFFSLISMELLSLEGLKACNSGMPIPALNPV
                     FYSGKSTLLGAEINRCKRFYESHSTPWALVIPDYLLDAEADKVLAQSGFLPIDQGVAM
                     YLSIGDLEISELVSDLLIRRMDNEMDAWILPTIPAFESTEEIAKIYRVRHQEAIEKSP
                     GIYHFSGFIQEQIVCSLTLTVMGDSARIDDVATFPKYQKKGYATQLILSVLQTLRDTN
                     ISWCFLEASAYGLNIYKKIGFRELFKNLYYEEQCQYEQN"
     gene            3772..4146
                     /locus_tag="AC06IO_000489"
     CDS             3772..4146
                     /locus_tag="AC06IO_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIO9873705.1"
                     /translation="MLSDPNLVLFYVKNPAKSEEFYKNLLNTQPIESSPTFAMFVMKT
                     GLRLGLWAQEEIEPKAHQTGGGMELSFQVSSNEMVDEIYKQWSDKEISIIQPPTQMDF
                     GYTFVGVDPDEHRLRIFCLKRT"
     gene            4303..4917
                     /locus_tag="AC06IO_000490"
     CDS             4303..4917
                     /locus_tag="AC06IO_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873706.1"
                     /translation="MVWDATTTNAISNAVHAFFLLLYLISACIHYFKKDHTFSLLIVF
                     FFLILLVLKVLGVYVHYYPSHLYLPPAWIAISLLVIMLNYLLVQSMQMPDLCRVIVVF
                     LSIIFTYLFLTHDGNYTYIALPVILVYLIAAYYSQAKVRIGFVMVVISNLIWIVTRHI
                     ANYLTGHEIPIEYRYDNDIYHILLILSTYVIYKGIAEGQWKHPH"
     gene            complement(4914..5615)
                     /locus_tag="AC06IO_000491"
     CDS             complement(4914..5615)
                     /locus_tag="AC06IO_000491"
                     /inference="COORDINATES: protein motif:HMM:NF012422.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIO9873707.1"
                     /translation="MNAFIYSQELFHFDAVRLLTSIPQSILVKNESLHFVAANDKAAQ
                     LTGFNNPNEMIGLNDAQLNCSAAALHEEIGQQDYSVLKGDSQYFLDIGYYSGKGSLTI
                     LFTSKIKIFDEKNRSFVLVSTTEIPMIALNSMLFSLINSIKNNKKGVQSYQIRNDIYP
                     IKLTNKQSECLFYLLRGKSMKEIAFILNRSPRTIEDHINLLKYKFNCVTKSQLIDKAF
                     QLGFGQIIPPALLAF"
     gene            5794..6162
                     /locus_tag="AC06IO_000492"
     CDS             5794..6162
                     /locus_tag="AC06IO_000492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873708.1"
                     /translation="MKKFLFSFLFLFSTFAFSQTFSLTKENIPTFNRVSWALKGNGQY
                     HTIQNVSGKTLRLDLWVDKVIAGTANTPSDNVYILCGEISQPVSAGTGIICDIPYPGL
                     AIIANYPYVNGVTGQFSIHY"
     gene            6182..6535
                     /locus_tag="AC06IO_000493"
     CDS             6182..6535
                     /locus_tag="AC06IO_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873709.1"
                     /translation="MKKMYFLVLSCFIVFSATASGILHKSFALITSSTYPWNLPTPGS
                     MTLYKNLSKNPVSFVYTNDKNSDTVVIRCPLNDIVLQANYTYVCWLPAGENAQIELKP
                     YYFRNGASGSYSLIL"
     gene            6785..8095
                     /locus_tag="AC06IO_000494"
     CDS             6785..8095
                     /locus_tag="AC06IO_000494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873710.1"
                     /translation="MSRTGEIRKMLATQMVQARLREYYGESIPPISESNLEEVFNEAR
                     QGRSYYNPHWDKFDQKIDIEEQAAILLIQAVDSIDLQFEDEDTVHKVLGDIIDETQQY
                     LRQKFPQGKNDLHACLNDCKKLLKSNLPNNAMSDQFRQSFRSELNQNPAEREAMWHSK
                     KAVKDAYEKHHTEHLNQLPLVERQAKINKIIELLDHRALHNAHNEKPKIIKDILSIVK
                     KMNPRDTKNIHRAFEKIHGVIENWDAKEGEHEDSQSLILKNLAVFISQTAHTMHENPE
                     QKKQRLEEAINLSSEILGKSPDETKQILRDKLQKSGFWHKLVPGDPWANCLKNCLKLT
                     NIDPPNLRNSPGALNAVNAFRGHEPTLTDTYQKLLQVPQLKTIVQPQPANSSGAKVST
                     PVPPSQQTATSHSAERRTLLSDYQIDDENTLKKIGQEKPGFKPG"
     gene            complement(8100..8174)
                     /locus_tag="AC06IO_000495"
     tRNA            complement(8100..8174)
                     /locus_tag="AC06IO_000495"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8138..8140),aa:Lys,seq:ctt)
     gene            8347..9108
                     /locus_tag="AC06IO_000496"
     CDS             8347..9108
                     /locus_tag="AC06IO_000496"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9873711.1"
                     /translation="MLKLFDAHFHIIDYRFPLIANQSYLPPEFTVEDYLQRAKPLNIC
                     GGAVVSGSFQAFDQTYLLTALSVLGPNFVGVTQLPATVSDEEIIQLNQHGIRAVRFNI
                     KRGGSESIHQLKYFAHRIYEMVRWHVEIYVDSKELGDLTNLLLELPAVSIDHLGLSQS
                     GFSELLHLVEHGIKIKASGFGRVDFDVKKALQTIAQINPDALLFGTDLPSTRAPLPFL
                     DSDIQLIQDLFDEELTEKILYRNACNFYSKNKPLA"
     gene            complement(9244..11082)
                     /gene="legS2"
                     /locus_tag="AC06IO_000497"
     CDS             complement(9244..11082)
                     /gene="legS2"
                     /locus_tag="AC06IO_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector sphingosine 1-phosphate
                     lyase LegS2"
                     /protein_id="HIO9873712.1"
                     /translation="MFVRICAMFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYF
                     LYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKNLPGVNQIIEKELNKELSATREK
                     LRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVGGGDG
                     KDSGALYAIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKE
                     GYGLLTHGGTTSIIEAMAAYVIRARAKGIDYPEIVVPETAHAAFKKAAELTGAILITV
                     PVDKKTGAVNPKVMSSYITRNTAVIVGSAPSFMNGIHDPVSELGQLAKKKNVPFHVDA
                     CLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAA
                     LNWSGGLYATPGILDGSTSGARVAEVYATLSYYGKNKYQEIAKSIIRLRNAIQKELTA
                     LVEEGNGLTSEDIYVYGNPQWSILGFRSNTCNAHFIADELEKRGWKLNLLQNPDGFHL
                     CLTHVHTLVRGFETQFIKDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIELQKEIC
                     KQYQKARLDFTAASHGSLGIFTTSPTEEDDGLRNRKAGEQKVQTSL"
     gene            11299..12405
                     /locus_tag="AC06IO_000498"
     CDS             11299..12405
                     /locus_tag="AC06IO_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9873713.1"
                     /translation="MNKMLALILQLLAIILIPALISCSSESNKPAGQILAEVDVALPV
                     KKKIVEWDEYTGRFQAVEEVDVRARVTGYLEAIKFKDGQTVKKGDVLFVIDQRPFKYA
                     LARAEAQYALAKAQYERAIKLQKEKFISAEVIDQRFQDVQVAETRVQEAKLNLEFTEI
                     KAPISGKISRDFVSVGNLIRMNDTVLTKVVSIDPIYFYFETSQNDLLKYIRLERAGKR
                     LSSEKEPTPVLIKLPDEKDYLHQGEVDFLDNVIDSGTGTILARALVPNPDNVIYPGLF
                     ARVKLVGSEEYEAILLPDKAINTDQARKFVYVVDNENKVKRVYVELGPLRESGFYIIR
                     SGLKGDEKVIIHGIQRIHGPNQEVKPVVIEVTEN"
     gene            12505..15663
                     /locus_tag="AC06IO_000499"
     CDS             12505..15663
                     /locus_tag="AC06IO_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIO9873714.1"
                     /translation="MKIALFFIDRPVFATVISIIIVMVGVLSYFNLPIEQYPQVVPPT
                     IQVQASYPGANAKTVAETVATPIEQEVNGVENMLYMDSQSTDDGQMRLTITFKLGTDL
                     DQAQVLVQNRVAVVEPKLPEEVRRLGITTNKNSPDLMLVVNLYSPNGQYDQTYIGNYA
                     ILHIRDKIRRIEGVGNVLVFGASEYAMRLWLNPDLMNTFNLTANDVLAAVRAQNVQVA
                     SGKLNLQPQKNQYGFEYHIETKGRLVKKEEFENIIVKSGEGGRIVRLKDIGRVELGTQ
                     NYLTKGYLDKYPAVALPIYQRPGTNALATAQEIIKTMKTIAKAFPPGITYDIAYNPTL
                     FVQQSIDAVFHTIYEAIALVVLVILIFLQTWRASAIPVVAIPVSLIGTFAVMQAIGFS
                     LNYLTLFGLVLAIGIVVDDAIVVVENMERNIQAGMNPRDAARKTMTEVGSALVAMGLV
                     LIAVFLPTVFLEGISGRFYQQFGTTLAVATAISVFVSLTLTPTMAVLLLRAHHETIKQ
                     EGLAWWRKPIYGFLHGFNRLMEGFSRQYGKLVATLTRKTALMTIAYGIFISITLLLFV
                     YVPRGFIPRQDQGYFIVAVQLPPGASLSRTDAVINKAVNKILAIPGIAHTVSFTGFSG
                     ATFTNSSNAGAIFTPLLSFQERQRMGINYNDILKRLRQELSTIKEALIVVIPPPPVRG
                     IGNAGGFKMMLQDRGGRGLDVLMEAAATMVNSANQEKATTSVFTFFENSTPRLHLKLD
                     REKVERLNVPYANVVEALEVYLGSVFINEFNYLGRTFRVIAQADSEYRHTEDDILRIK
                     VKSNNGDMVPIGSVAQIENTVAPSRMPRFNLYPAIDLQGDVAPGYSSDEALVTMEKLA
                     QNNLPDGIGYEWTEIAYQQKMVGNTALMAFTLGVIFIFLVLAAQYESWSLPLAVILIV
                     PMCLFSSMLGVKILGMENNIMTQIGFLVLIGLASKNAILIVEFARQLENRGYNLWKAA
                     IQAAKLRLRPILMTSFAFILGVFPLIISTGAGAEMRRALGVAVFSGMLGVTFFGLIFT
                     PLFYVLISRLSRYKRKLTRPEK"
     gene            15757..16206
                     /locus_tag="AC06IO_000500"
     CDS             15757..16206
                     /locus_tag="AC06IO_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIO9873715.1"
                     /translation="MNSSTSGKNNQQKNPLPTYFSDPPLQHVDLFKLSSYPVLDVEEA
                     IKFTLTEENLAEMLHTMLNHSLPDEVAEMKKFHDLGDWEKTQDIAHKIKGGCVYVGTV
                     QMKMACQYFERYWKSGETDLLEALYQQILHTIEENVRYIKNWLDKRK"
     gene            16509..23459
                     /locus_tag="AC06IO_000501"
     CDS             16509..23459
                     /locus_tag="AC06IO_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIO9873716.1"
                     /translation="MELKKQDFNYTVIDLFEAQVKKNPDFLALKDKTTAYSYLALNEK
                     ANQFARSLKKKSVKEGDFVAILLDPGVEFFICILAIIKLGAIYVPLDTLAPQTRLHEI
                     VKDANPGIVITHDEYQPLLEGIDTEIYLIKQLHLESLSQSKKNIKNTVMPTCPLYMIY
                     TSGSTGKPKGVVVPNRAVVNLAIIDNIIAIQKGEKAGQFCNLAFDGSVFETWVALLNG
                     AALHIIPNEARRNHNQLKKIIKEFQIKYLLIPTGYFHQLMKSFPETLDLLHTISFGGE
                     QVNVHLLKNFIDYRKKADIPAKLVNVYGPTEATVFSSCNIIHSEKEYTDEQLASIGFK
                     TANTKTYILDDNRRRTEEGELWVSGVHLALHYHNSPEQTEEKFVKNPFSRNELYSRMY
                     RTGDKVRQLPSGELLYLGRVDDQVKIGGFRIHLTEIEQELMNHKAISLATVTVEIGGG
                     AHKFLTAYLVFKSKDTIVDADEIRNFLSQKLPPYMLPAKYVMVEKLPLTAVGKIDKAK
                     LDKIAHTDLSFHIDTSSSNSIEETIKNIWRHLLNRSSIDTHKNLFELGANSLLITEAC
                     AQINKELQCDLQIVDMLTHPTIHKLSRFLEGDLDTHLARKIKKAASQDIAIIGMSCRF
                     PKARNIDEFWHHLCQGKDCLTRFDENTIPHHLLAQEDYVPVKGILENSDHFDAHFFGF
                     SPIDAKITDPQHRVFLECVWEALEHAGIAPGKLDSKIISVFSGMTDSTYLNENLLKNQ
                     WFCNEFDQFQQRIATSMGMLSTQTSYRLNFKGRSVNINTACSTGLATVDHACQDLILG
                     QSDIALAGASSIVVPETRGYFFQNGSIVSPDGYCRPFSSEANGTVFSNGVGVIVLKRL
                     EDAIKNRDTIYAIIKGRGVNNDGIDKLGFTAPSMSGQISCIRDALQQAQISAEEISFL
                     EAHGTATALGDVIEIQALTSVYREQTDKTQFCALGSVKANIGHTDVTAGIAGIIKTAL
                     CLYHKKIPPLIHYKKPNPDLSLPESPFFVNTETQDWDAASKRYAAVSSFGIGGTNTHM
                     ILSEHEHEEKMASVAPLKEELILLSAKTEKALQQSTYNLKHYLKKCDDSNLGNVAYTL
                     YRGREDFQWRQFAVGKTVEEICAELKGKKPLFFNQDTHHSIAFMFSGQGMQYTQMAME
                     LFKTIPLFAKYVTSGAEIAQSYLHCDLLSILHDSSSKKLKETQYAQPALFIVEYALAK
                     LLMECGIIPEMLIGHSIGEYVAACLAGVFSFEDGIALVCERGLLMSRTPKGAMLSLDA
                     TQEQLDSYLKMVDVDIALHNATNHFVLSGSEPEIEKLESYLTAHKKPFQKLKVNHAFH
                     SRFMESIEMPFKEMFTNIKLSAPQIPIISNVTGDWLSAGEAINPDYWYRHLRETVQFC
                     QGINRILQDKHPIFIEVGPGQSLYGFVGEISHKKANALFTLPGCQKRLSDRIQLLSAC
                     GELWAKGVNIRLESLVDNDNRKFIPLPTYPFQRQRYWVDPDKKNISVNEACSLYKPVW
                     SHEKIAHVTPANLKNFDYVIFKDKTGIGQKIINILKNHDIEPIVVELSEEFRQLSKTH
                     ISINPGEKSDYNQFVSSLKNTLRHPIIFHCFSCDSSEDSFLDTQQIENRLKLGFYSLL
                     YLTQAFLNDLGTNIPIRLAIITSGTQNVIGTEKISPINGSLVGASRVIPLEHHNISCE
                     LFDINLYETTQNITNAIIDIVSSSTQAAQDLPYKLTAYRNSYRWELSYSPNHNKTVLQ
                     RLTDNGIYIITGGLGGIGLSLCHSILKNTKSPTFILCSRTPVIDESDWEKIATDTSHP
                     HHHKVLQLKKLKELGATIVCYQAHIGNDKDVDRFITFCKSRFKTINGLIHAAGVAGGG
                     LLQLKTKENADNILLPKIHGTYHLAKALREFHLDFVVFMSSIASITGERGQIDYCAAN
                     ACLDTFANSNLFYTQCLLSINWNTWRDVGMSVETQRPEDIDFFARGNTISPLQGQELF
                     LKALSSNCSNLIVSNYNIEDYSSLILDNNPADTEEHKVSRDTFSMSHEFCAPQGSIEK
                     RLAKLWQDNLHIVDIGRNDDFFALGGHSLKALHLIENINSILGVNLSIQHLYQAPTIA
                     KLAELIYSKNPENQIEIVIPLKETSSKQHPFFFCHPASGMVYCFDDLASFWDYSISLY
                     GLQDPSVSSGKLIFESLSSMAKAYTQAIKKVQPHGPYFLVGYSFGGSLMHEVAHLLTQ
                     QNERIGLLAMIDSWCTFSSKQKSKNHFIEAYLSKELQASRNLADLAWERMELLLNHKP
                     TKINQDMVLFKATDLNQDYLDIDDPKNGWGQYNLGQIICHTIEANHDTIIRQGYQFIA
                     QKLIEYRDIE"
     gene            23456..25486
                     /locus_tag="AC06IO_000502"
     CDS             23456..25486
                     /locus_tag="AC06IO_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9873717.1"
                     /translation="MRSQTIRLTQKDFPIFGKAFSLVNISFLVINENMELEYINDTAK
                     KKLKINWSSTDRKSFFQLWELANLTPILTKKGDLIPNKVLQVGSYSIQWQKTVAIVNE
                     KHYILLMDKDVATTSHLFEEIGAAIYGEIGYAPKNKISVKEYIQELTNYYTSVIDKIP
                     CYVYWKNTSFEYMGCNEMAAKFFGFKSTSDIIGKNDFDLFQDPDFAKNYQEQDKQVFS
                     TGEPILKMPSDLKDRNGMVTNTLVSKVPITNLSGSIVGLVGITVDVTELTKAKEAAEA
                     ANQAKTAFIANMSHDIRTPLTGVIGMSELLENTVKDPEQKEEAHMLHDSGEELLNMLN
                     DILDDVHAGFMDKEDIRIETFDLYQCIEDLVKLERPTTVMKHLELIVHIGKSVPRYIQ
                     SDRKKIHRVLLNLLGNAIKFTPKGQITIQVACLEVSGKNIHLQFKVSDTGIGIPTSLQ
                     EKVFERFFRISPSYKGIYKGHGLGLHIAQSYVNLLGGHIALISEEGKGTTFYFDIPCQ
                     TGTQKKIKTNKLTNSSKKIDSSLPTSSNSQLTPKLSSKTEENSPYVLLVEDNNIALKV
                     LELILSNEGYRFKSASNGEEALALIQTESFDLVITDLGLPGISGCELSVKIREWEKKH
                     SKKPLPVIGLTGHTKDSSEKGCLASGMNNVFTKPVTPELIRNIINKYLLGEK"
     gene            25717..26625
                     /locus_tag="AC06IO_000503"
     CDS             25717..26625
                     /locus_tag="AC06IO_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIO9873718.1"
                     /translation="MSQQIGNEFFEADMDMHQHYLDILNCVPDVVYWVDKDCHLKGCN
                     QNFIKLLGLKKIGDLKGTPYDQMAKHMKWSEKRIETFKLDDMKVLFSGESQYDIEEEP
                     VYNKGKEAIYYKSRRVPLFDKENKVCGLVVVLTDITDLKKLEAHIQQTHSPRNIKRTQ
                     KKRVTEPKILMVEDNFVAQKVEEALLKELQCQVDIADTGDKALNLFDPGKYDMVFMDI
                     GLEDTSGYLVAKKIRQKEQNTNYHVPIIALTSYQADVVKYDVEDYFMDGVLTKPLTSE
                     QADQLIKHFVHKEDIMVSGLKSAREN"
     gene            26907..27668
                     /locus_tag="AC06IO_000504"
     CDS             26907..27668
                     /locus_tag="AC06IO_000504"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9873719.1"
                     /translation="MIWDPERYVLGNYIQNKINDVFLKTIHSKLSGNILDIGCGDGHY
                     TSLLAGKVKHGQILGIDSSEQMIMHANQQWARTGLSFEVHNIEEFHQPQSFDLALSFW
                     CLHWTNIHISFPNIFHLLKREGKLYAVMSSFSDHSILQTWKELAKQNLYRDLTEQYIS
                     PINEQYFYGVINLLNRLPFKRIKLDLKTCRVHLPHIDYYKNLLLTMPFIKRVPSEITD
                     TLVEDMLKAFQTICDRKYGGQLYYETRPIFLEAIK"
     gene            27693..28727
                     /locus_tag="AC06IO_000505"
     CDS             27693..28727
                     /locus_tag="AC06IO_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIO9873720.1"
                     /translation="MHLNFNTTPGMNIPMVIANQDNLKASPTPLLHFISEYHDEVIQK
                     LNQYGAIVLRGFSCQEENCFSKAIELCALGTRCSTSDYDLPRTVLANEIYTSSDLPAH
                     IPLPLHHEKPRSPKPPNHIYFCCIIPPQEGGGTIFANAEEIWIDMPQDIQNKILEYGV
                     QYKQFFHGHSVKYRVLRKILGNHCARSWVDYFGTEDKTQIEQNLTQKQVVWDWINHGI
                     DLIILNYLPGALKHPLTDKIAWFNSSAYLNYYSNLNYGELKNLRSFKYWASRYLILKD
                     MLPMICHYGNGQEFSAKEISEINQVIQRHTRVFHWQKGDFMIVDNFTFMHGKQAHVGE
                     RLLYSCMTAY"
     gene            28985..40327
                     /locus_tag="AC06IO_000506"
     CDS             28985..40327
                     /locus_tag="AC06IO_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9873721.1"
                     /translation="MVNVTMKNKTKDLLNEPIAIIGMNCQFPGIDSDIEDVSAFYDML
                     MKGQTPIKDVPENRWNIEEYYSADRKKADKIISKKGGFLDNTHLFDAAYFKISSAEAK
                     QIDPQQRLFLEVAIRALNDANIPLDSLKDSNSGVYCGISTHEYSQLNYKDHIKFNAYT
                     PIGIANSAAAGRLCHFLNLKGPSMAVDTACSSSFTALYLAATALRNLQCDMAIVGGVH
                     LSLCPESFVGLTKANMLSATGQCSSFDSKADGFVRSEGCGVVIVKRLSDAIRDNNKIY
                     ALIKSMVINQNGDGTGLAAPSTNAQIAMHQAALEHAHLTAGEIDYIETHGTGTTVGDP
                     VEFNAIQSIHQGHHSPQKPLIVGALKSNIGHTISSSGIASLIKVLCSLQNEAIPPNLH
                     YSNPNSLIDPESIPALLPVEAIPFLKLKNKKRYVQVSNFSFTGTNVSVILEEAPELKS
                     HESKQDDNEPKCFVASANSELSLKQLMSRYEHYLKNSSSTLGDICHTLINCRDHYKFR
                     CAIIVNDKKELIKKMESKDYEIKKVVLQNNPRTVLNDAKAIYEAYLSGINISTDDKNT
                     AYNKVDLPLYCFDRKPYWHEVRKHVKNDHWLDSLYQQPMEQQVATIKAELASQIKDLL
                     KKDNIDPHQDLETLGLTTSLLESLDQILQDMFSPRYKVPSLQSLRYLTLDKLARHLQK
                     MIMPPVVYRQPSINVLNVEPIAIIGMSCRFPKAANVDEFLSLLEKGESGMIDIPLERW
                     DNEKFYDPDVDALGKLYIKQLGFIDHVNHFDAEFFNISPREAKLMSPQLRVFMETSYH
                     ALEDANLPLDAVKDTNTGVFVGVGTNEYPRVLAYQGVTLEDLNIYFATGNVLNALAGR
                     VAYSFDFHGPIQAIDTACSSSMTAIHNACLSLQSGDCNMALAGGVNIILSPDSNITLS
                     KARMLSPDSRCKTFSEDADGYGRSEGCGVVVLKRLSDAIKDNDNILAVIKGTSINSDG
                     KSGGFTVPNGAAQEEVILSALAKANLSPGDIDFIEAHGTGTPLADPIEVNTLTKIFSE
                     HHNKENPLYISSVKTNIGHSESASGVASVIKTVLSLKTKTIFKHLNFKKLNPAIQLIN
                     TVIPLDKTDWTKKQGLRCAGVSSFGFSGANAHAILQEAPEDKKEARTLPEESLLVLSA
                     KSKTSLELLLASYQNYLSNTREEFADICYTAATCRSHFMYRVAIKAQNAGQAANIIKG
                     NGYTICHITKEKEWTQQPDTLAELQTAYLDGFPINWSEFYRSLGHHFAKVKLPLYEFE
                     RIEHWFGDKNKLKDAPLPKDWCFQIQWQNEPCDKNNRKLRGNRWLLIGPRHLADGFKA
                     QGLEIVYEDEEYSRNKLEGIIFAESLHLAPPNIEANIDFQKKTLKKLLNLIKELDNQS
                     IDLQLLILTSNGIAELANDKHPVNISNSPLIGFCKTLALELPQFKTILIDMDKIEEDK
                     YPTQVIEEINYNHGSRYEHIVAYRGGERLASRLKKTPLEDRRRLLHGVKGRYLIAGGC
                     GGLGLVTAQSLLSSGARELILTSRNIDKPRIKEAIYKIKSHYPGANIRVVSLDITDRE
                     SLKNLLSELNADGQLKGIIHAAGAAIKAPLLEHQDEDVDYLFSAKVKGGWFLHELSLD
                     CNLDFFVVYSSVSSVFGSNKESVYSGTNSFLDALIAERQRMGLPGTAVQWGPWGEVGM
                     AQKRSRDEGLKQALISNAQGHVIIKCLINDEFKHATIISPEYLKFMLDFVPKPLPGFY
                     GQLDEELEKIPQQHHQSPAKNLSPWLNQYLEISEEERFKACKAMVSDICAGILELTEN
                     EDLNEDEGFFELGFDSLMITELASELKKKLEPFLKVTVTIGFNYPSISKLAKYIESEL
                     DKNLVKIQSPKSTPKPIDDGIAIIGMSCLLPNAPDIAAFEKLLEEGLSGIKDIPNDRW
                     DNSQYYDPDMEAPGKSYVTKLGLIENIKYFDAAFFGISPREAKLMEPQQRVFLECCYK
                     ALENANYPSESLRGSLTGVFAGVGPNEYYAQLEKSGFSNEELSAYSITGNVLNLIPGR
                     VAYTFDFKGPSISVDTACSSSLVAIHYACQSLKNKEIDYALAGGVNILLMPESNVTLC
                     KAKALSPDGQCKTFDERADGYVRGEGCGVVLLKRLSDALRDNDHILAIIKGSAVNNDG
                     KTAGLTVPNGKSQEEVMKKALGQTELSRTDISYIEAHGTGTPLGDPIEVDAINKIYGH
                     QRNKENPLYLGTVKTNIGHLESASGVAGVIKTVISLQRKTIYKNLNFNKLNPNIKLDG
                     TQLALEKTNWKTNTKLKYAAVNAFGFSGTNAHVILAEFPKEEKPKTSKPSQKHLLVIS
                     AYSKTALDNLTLRYQQYLETTEHHFGDICFTAATCREHYHYRLALTAENAIEASRMLG
                     AGQFALSYHENITLDLQNDRALNSLLMDYLQGKPVDWKSYYQSLKGEFTKILLPNYTF
                     DRSEYWLDKKNNDSIATHKGEQHPLLGQMLTMPGNEYLFHQKLDLENLAYIKQHSVFE
                     KVIFPATAYIESGLAAAKSIFQSSAFQIEKFNIERPLHPKQGQEFQLQVKPKMDGQYK
                     INLFAKQENDWQTFCEMEIHAKPTAARESIHIDVLKSSFGNKVELSDIYEQFQKSSLF
                     YGDEFRVLQEGYVRANSVLAKVTLTKASQGQDYYYHPVLLDGAMQSILLLSVKNDENK
                     NFNAENVTYIPYAFIRMTVFKEAPRSLWVHLTKQNTEDASDLCVNINLYEQSGLLIAQ
                     IAGLKLRKVTRSHFISYDFVLNHLYYTEWSPVPLKTLPQQKIPDLLAITKNPGKAKEI
                     LDGLNYQLVERIDDCTNIEHSNIVFLYEQGQFHDLFHCCQILFRLRPKRFILVTEHAY
                     AIQNHDQVNPYHTMACSFWKSFRNEYEPNKNYAIDLDSKSRLATALMYLLNTDTHDTQ
                     IAVRESLFLPRLKKKQLASNSAPEILIDGNATYLITGGTGGLAKPLMEYLMRRGARHI
                     AITSRTDYSDDIKALIDKAKQNQIDIKHYMVDAGNYQKMEQVIGEIQQGPNPLKGVFH
                     LAGLIQDGLIVNLNDEAMQKVISAKMDGALNLHQLTQNIPLEWFVMFSSSASLLGARG
                     QANYAAANGFLDGLAHLRRQQGLPAISINWGPFHTTGMAANLTHTLQHYGFLPLDKDN
                     IDILDVLLPSQLPQISPCPMNWDVYCKHTPKQTELSALVKAKPLPDQSFLNALRQHSK
                     EESISILSQALREIAADVLALDDSEHISNHQDLFSMGLDSLMSMDIRNRIYDKLQCQT
                     LSLPIEYFINTPTINKIARHISDELDHVFDSHSDYHPTENPSEREIALCDFQYVFWVL
                     NKLDYHFNIGTQIQLHGRINKDYVQRAFEFVVNQNSVFWIHFNKDKPTQKLHKVGQFE
                     LIYQDISLNHETKALNHEFQNNILRAIPLTKQPLIRVYLYKINNELHELHIVIPHIIV
                     DDASCEMVFCQFKNNYEKLTLGKELVSNPEKGSYFNYVKQNNNHYQKNLKNKIDFWQN
                     YNKGFKMLSFGNRYHLSDTSKQTKHLFHYPIDPQHAEQFINWHKTQNINVSTGLIAAC
                     QIVFYKLSHQNKIPIILIHSGREGGEYKSTLGLFSEYKRINLTLNENSQFIDCIHSIE
                     EQLLKTAPYQKCSHLIKDSGLKGSSLSFGQYLTLALNKMFMLKHFKESKLHSIIIDYY
                     LKYLSRLLSFKKNISIKQRLNQLFKLDMSLQKPERLRILVSVTPSFFTKERPDMSFAN
                     LDYSYPNHFGCMDRPIGNRTLWIYFSKNQDGEYQISINAPLTIECKNKIGSSLSQFIT
                     TFVENNNCNIAELIQAMEDSSIAS"
     gene            complement(40330..40731)
                     /locus_tag="AC06IO_000507"
     CDS             complement(40330..40731)
                     /locus_tag="AC06IO_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIO9873722.1"
                     /translation="MKSLRMAGLLFLFLSSLVSIHVYAAPNTGASNAEMKDVCQLAGG
                     TFTSTIEGNWACCWDNWGCYGCVQGVCKMKCHTQRCRRANGQSAVLQPGEVKIKGKFG
                     TDIVVPKVSTQPSSSNPKQIKNNNTTVNQAQ"
     gene            complement(40962..41915)
                     /locus_tag="AC06IO_000508"
     CDS             complement(40962..41915)
                     /locus_tag="AC06IO_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIO9873723.1"
                     /translation="MTSISEWWMWLGFLAFVLIMLFVDLFVFGGRKAHRVSTKEALSW
                     TILWFTLAIIFNFLLWWYLVENFTFSVANEKALEFFTGYLIEKSLSFDNIFVFLILFE
                     YFAIPAKYQHRVLIYGVIGAIVMRLILILLGIWIVNQFHWILYIFGFFLMITGIKMFI
                     FADKKPELAKNPILHWMRSHLRITQELHGERFFLRKNQLVYVTPLFMVLVLIEISDLI
                     FAVDSIPAIFAITNDPFIVFTSNIFAILGLRALYFLLVNMHKRFYFLKYGLAFILVFV
                     GFKMLIAPWFKIPIFIALGIVMATLVFCILFSIWHRKNEVT"
     gene            complement(42030..43895)
                     /locus_tag="AC06IO_000509"
     CDS             complement(42030..43895)
                     /locus_tag="AC06IO_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HIO9873724.1"
                     /translation="MPKHLFYGVFLILLSMKAYSQDAVSGDIAKRTESIEKKGIVYHN
                     VIDCDYKISPEISHIDSDIILNWAEYAAILSFNFNFASIEDQLNQLHSCYTEKGWGQM
                     QNALHKSNNIETIKAEKLKVKGYKDGEAQIIEVLDNRWKIILPLKVVYENDKKRVTHF
                     LNAYLTIGWKNKDSLGIMQMIAIPRLAPLAQKVTPADEVIKSMSIILAERKKASLDSA
                     RKITDSFFVPLFSNHGEYSKRNLALSNRVSQRVAIIQPMPNLNPTHNLVRMQSFDMPE
                     SAKTPYLALSQQRSFVNDSDSNKSTLSDFGNKSRKLYPNWLSQIGLHSSKLQLNSLIT
                     RINPLPLWRAFSQYKLSIRDKSDNIEAINKQKPILSAQIEGEPQFIEVKENQWDVKLP
                     IKVVYQKDNERIIQRLDVNLALDQKMINHLTVKNKSLDSNRVLQSLKSILASSSLQLS
                     PQLFLGSQNIQLKQPEQSKPKETVSCHYKIPEETIKTDKNIILQWAEHAATQSFAFDF
                     TSIESQLNKLQSCYTENGWIEFNSAMMKSGNIEAIKMQRLTMTSEVNGVAEFIETREN
                     HWIIALPLKVTYKNDTMQVSQLLNIQLTVGRKTNGEFGVIQLIATLDPSSSSPPS"
     gene            44430..44930
                     /locus_tag="AC06IO_000510"
     CDS             44430..44930
                     /locus_tag="AC06IO_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HIO9873725.1"
                     /translation="MNTTSLSLTPLLRHSVGFERFNDLFESMRNADDSTYAYPAYDIE
                     KHGEDNYMITMAVPGFQESDLNIMVQNDQLRVSGRIQEKETKESEYLHRGIVTRAFEQ
                     TFRLADHMKVTGAEIKNGLLSISLIREIPEEAKPRIIPIKSISDQESNKKSKTIEPES
                     KKKISN"
     gene            45112..46632
                     /locus_tag="AC06IO_000511"
     CDS             45112..46632
                     /locus_tag="AC06IO_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIO9873726.1"
                     /translation="MKRGLFVHLKHDLPASIVVFFVALPLCLGIALASHVPLFSGVIA
                     GVIGGVVVGFISGSALGVSGPAAGLVAIVISSVEILGSWEAFLLAGVIAGILQLIAGF
                     LRGGIIAYYFPSSVIKGMLTGIGIIIVLKQIPHLLGHDVTPLLLSMSDGELDINDIRE
                     ALDDINPEVVIISIVSLLTLIFWEKVLVKKHKLFEMIQGPLVVVILGIALNLFYEHRL
                     TSFSLDPSELVQLPKFNKASELFNYLTVPDFSLITHLKIYKIAILIALIASLETLLCV
                     EAVDKLDPYKRVTSTDRELKAQGVGNILSCLIGGLPITQVIVRSSANVAFGAKSKLSA
                     ILHGLLLFICVISIPEILNEIPLASLASILIIIGYKLAKPALFKQMYKMGWEQFVPFL
                     ITIIGIIFSDLLFGITLGFSVAIFIILRHHFLNSHDIIKIRGKNKTQYCLKLAEEVTF
                     LNKGSIINELKNIPEGAEVIIDGSRSKIIDHDIKEVFQNFILNSKTKNIQVKLIGI"
     gene            46636..47262
                     /locus_tag="AC06IO_000512"
     CDS             46636..47262
                     /locus_tag="AC06IO_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HIO9873727.1"
                     /translation="MWTLTKEQQQAITPEKAIELLKEGNKRFVSNLKLNRNLIQQVNE
                     TSQGQFPFAVILSCMDSRTPAELIFDQGLGDIFSIRVAGNILNDDILGSIEFACQVVG
                     VKLIAVVGHTQCGAIKGACDGVKLGNLTNLLNKINPVIQEAKKLDAKHDVHSPEFLNC
                     VTSLNVKHTMNEITQRSDIVHQLLNEKRIAIVGGLYQLETGEVQFFDE"
BASE COUNT        15396 a         9251 c         8798 g        13913 t
ORIGIN      
        1 caaatgttct ttaaatatta gccattttgc gatttaataa agaaatagat aaccctgtct
       61 tgagaactca tgtcggtaat aaatggtaag aaaataataa ctatcattct ggggattgca
      121 tgcctttttt gttcaataca cccggtaaat tctgctccgg atttctcaaa taaaccgcat
      181 gtgagggaaa aaaacaaaaa tctccttggt gtttttgtag gcggaaatca tgctcaaaac
      241 gatgaagcgt ttacctatgg atttgagtac caccgagtcc tttccatgcc ttttgggttt
      301 agtgccgtta ttgaacatac acccgttaat gtggaacaga ataatcaagt agaactgata
      361 ggccttggca cattaaacgt attcaggaac ctgacttttg gcataggccc tggaattaga
      421 tatgaaaaag atgaacccaa ccgcatggtt ggtcgattgg ggattggata tatcatacat
      481 tttcctccgg atatagaaat caccccgaat atagatcttg attttattga agggggtgaa
      541 aaagagctga tctttggtat tacttttggc aaacaatttt aaatagctct tttcccctat
      601 atgaattgtc tctcccgctt tctctctttt taaaaatttt ctgattgata atcacgacta
      661 aataatagct ttttttagaa gaccggtgta aatttatgac tcttttttct taaataatga
      721 ttgatatgat ttcaataaca acttattggc aacaatttaa agattgcatt gatcaacttc
      781 gttcatctgt agacaacaaa gaatactcta atcaaatagc caccgcggat cttatgatcg
      841 agattggctt gacaatagaa caactgggga ttactcaaat ccaacaggta ggagatcgac
      901 tgcacgaaaa agccaaattg ttcggatatc gagtcccatt tgttacccct gaaaaaaaat
      961 atgaattttg tttacccaaa ataactaacg ctgaaaccct gatttttgca atcgtttact
     1021 atcagcacct taagaactat gaaaatgtcg aataccaagg ttctatcaat tggtttagat
     1081 caaaagtaat ccatggtgtt aatgaaaaga aaatttcgac ccgtgctcaa tccgaagcga
     1141 tgttgggaga aattagacaa tattgtcaaa gtcaaaatat tgcctttaat gaatctgaat
     1201 tcataaagaa aaatgacgag tcagcaaaag agtatacaac aagattgttc aagaattttc
     1261 ataccgataa aactgcaaaa agtgaaccaa ttgggctaaa acaacttaac gaatcaaaag
     1321 aatccaacct gtccgatgag ctagccaagc ttgaaaaatc ctttgaagat tcaagaaata
     1381 aaagaaatca attgattgtc aaaatagatg ctcttgcaaa gaaactggca gactatcatc
     1441 tcctttcgca aaaccaccat aaattaaaca atgaatggaa taacaaatgg ttttttacaa
     1501 aatttttcga ctggatcatg tcctggttct ctaatgattc atttatcaaa aatttaaaaa
     1561 ccagctatga gcaagtcatt aatgctgaac atgagctgaa gcaagaattt caccccttcg
     1621 acaccgcgga tgcctatcga gctgaattaa aaaaacaact ggaagaaact cacattgaat
     1681 gcgataagaa tcaaaagcaa attaacgaat atcaattgaa acagttagaa tacaaacttg
     1741 aacaaaggtt ggaacaaaag ctgcaagaaa aactacaaaa ggacactgct gttgaacaaa
     1801 aagaacctcc cgttacaaaa gtgctatcag aaatcaaaca cgaaaccaat ggcagtgatt
     1861 tctcttcaaa tttaaatcat tattatggtt tctttaaaga gcacttgcca aatcgagaaa
     1921 cgttgggagc aattgctgtt ggtgtcgcag ccattgccat tcagaatttg atgtaaaacc
     1981 gtttgaaata cgaggttctc tcactcagga ataagtggga gttcaacacg tctttcgcat
     2041 aagaagcaat cttaatccgg gaagaaagta aaacttgatt cccggcatta aattttacct
     2101 gttcaagtta acacctcaat aacatcgtgt tgccatcagc ctgggatatt attttgcttt
     2161 cgccaatcat gaagtaacac cgagcgtcgc tttgcataac agacccccag gttttgaaca
     2221 tctacaattc tatcggctct aaaatgacga aaatcctttt ttaattcaca ccaggcaatc
     2281 aaaactctta cttgatcaaa atacccaagt ccaattggcc aaatcgtacg tgttgaaaca
     2341 tttcctttta agtcatggta ttgaataaac aatttcagtt cttttcgtat tgccttcctg
     2401 actaaggcca gggattcatc ttctcctgaa atcacttcac caggccctat taataaactg
     2461 gatagctcca attgctgccg caaatcggca ggcagcacag aagcgatttt agccaaggca
     2521 ttatgcgcgg cctgttgcaa gtgagtatcc gtttttttag ccacccatct ggagcctaaa
     2581 acaagagcct caatttcctc ttcattaaac atcaaaggcg gtaacataaa acctggcttt
     2641 aacacataac ccaagcctgg ttcaccctct atatcagcac cttgagcctg taaaaccgca
     2701 atgtcccgat agatggtacg ggtacttaca cccagctcat tggcaagaaa ttgtccgcta
     2761 agaggataac ggtaacgccg caaaatttgc aacagatgaa agagtctctc agttttgctc
     2821 atattggcat tgctcttcat aataaagatt tttaaacagt tcacgaaaac caatcttttt
     2881 ataaatattc aaaccataag cagatgcctc taaaaagcac caggaaatat tggtatctct
     2941 gagagtctga agtacagata aaattaattg tgttgcatac ccttttttct gatatttggg
     3001 aaaagtggct acgtcatcaa ttcgcgcact gtcccccata acagtcagag ttaatgagca
     3061 gacaatttgc tcttgaataa acccggaaaa atgataaatc ccaggagatt tctcaatagc
     3121 ctcctgatgt ctcaccctgt aaatctttgc aatctcttcc gttgattcaa atgcagggat
     3181 agtgggtaaa atccaggcat ccatctcatt atccatgcgc cttatcaaca agtcagacac
     3241 aagctcagaa atttcaagat caccaattga caaatacatg gcaacgcctt gatcaatagg
     3301 taagaatcct gattgagcaa gtaccttatc tgcttctgca tccaataaat agtcaggaat
     3361 caccaatgcc caaggggttg aatgagactc ataaaagcgt ttgcatcgat taatctctgc
     3421 cccaagcaga gtcgatttac ctgaataaaa cacgggattt agagcaggaa ttggcattcc
     3481 actgttacaa gctttcaatc cttccaatga taaaagctcc atactaatca gtgaaaaaaa
     3541 atgccactcc atttgctgga aagtttcaat acggctgttc ataaaataaa ctcatcaaaa
     3601 tttttgagcc aggataggct aaattttgtt ttcataataa caccaaaatc ctgtttcatg
     3661 tgttttttat tatcaagaag gtcaatatta ggcacttaaa acactactga caaaatctgt
     3721 cattagcttt gtttatactg gctatgatta ataaaaaata aggaaaataa catgttaagt
     3781 gaccctaatt tggttttatt ttacgtcaaa aacccggcaa aaagtgaaga attttataaa
     3841 aatctgttaa acactcaacc tattgaatct tcaccgacat tcgctatgtt tgtcatgaaa
     3901 acagggcttc gtcttggatt atgggcacaa gaagagattg agcctaaagc tcatcaaaca
     3961 ggagggggta tggagctgtc ctttcaggtc agtagcaatg aaatggtcga tgagatatat
     4021 aagcaatggt ctgataaaga aatttctatt attcaacctc cgacacaaat ggattttggg
     4081 tacacatttg ttggtgttga tcccgatgaa catcgtcttc gtattttttg tttaaagaga
     4141 acataaacga ttggagtaat gactaatcat agcttgtagt atggctttta agtgactgat
     4201 atcacacatc ccaacctgct acaagctaca attactatta gtacattaac aaacatcagg
     4261 atatcagacc ctgacagctg tattttcaaa gagagataca gaatggtatg ggacgcaaca
     4321 acaacaaatg ctatctctaa tgcagttcat gcgtttttcc tattattgta cttaattagt
     4381 gcctgtattc actactttaa aaaagatcat actttttcac ttttgattgt attttttttc
     4441 ttaatcctcc ttgttctcaa ggtattggga gtttatgtcc attactaccc ttctcatttg
     4501 tatttaccac cagcctggat tgcgattagt ttgcttgtaa tcatgcttaa ttatctgctt
     4561 gtccaatcaa tgcaaatgcc cgatttgtgc cgagttatcg tagtatttct atcaatcata
     4621 tttacttatc tgttcctgac ccacgatgga aattatacct acattgccct tcctgtcatc
     4681 cttgtatatt tgattgcggc ctattattcg caagcaaaag tcaggattgg ctttgtcatg
     4741 gtcgttatat caaacctgat atggatagtt acaagacata ttgcaaacta tctcactggt
     4801 catgaaatac ctatagaata tcgctatgat aatgatatct atcatatcct tttaattctc
     4861 tctacttatg tgatctataa gggtattgcc gaagggcaat ggaaacatcc tcattaaaaa
     4921 gccaataacg caggaggaat aatttgccca aaaccaagtt gaaacgcttt atcaattagc
     4981 tgtgatttag tcacacaatt aaacttatat ttaagcaggt taatatgatc ttctatcgtt
     5041 cgtggtgaac gattcaaaat aaaagcaatc tctttcatac ttttccctct taagagataa
     5101 aacaaacatt ctgactgctt atttgttagt ttgatcggat aaatgtcgtt tctgatttgg
     5161 taactttgca ctcctttttt attattttta atggaattaa tcaaactaaa taacatgcta
     5221 ttgagtgcta tcatagggat ttcggtggtt gacactaaaa caaacgaacg atttttttca
     5281 tcaaaaattt ttattttaga cgtaaataaa atggttaaac tgcccttacc ggaataatat
     5341 ccaatgtcta aaaaatactg actatctccc tttaacactg aataatcttg ctgaccaatt
     5401 tcttcgtgaa gagctgctgc agagcaattc aattgcgcat cgtttaaccc aatcatttca
     5461 tttggattat taaatccggt taactgagct gccttatcat ttgctgcgac aaaatggaga
     5521 gactcattct taaccaaaat cgattgcggg atactggtaa gtaatcgaac tgcatcgaaa
     5581 tgaaataatt cttgcgaata aataaatgca ttcatggata tcttttaatt agctccgata
     5641 atctgtcaaa atatagattt agtatcatac cctcaactcc ttttaactcc tattttttaa
     5701 aaaataaacc cgtaaaaagc acggtatctt ttagaaaaat ttcaagctat tttctacatt
     5761 cagtcaatga aatactttta aaaggagctt atagtgaaga aattcctctt cagtttttta
     5821 tttctgtttt caacttttgc tttttcccaa actttttctc tcaccaaaga aaatattcca
     5881 acattcaata gagtaagctg ggctctaaag ggaaatggac aataccatac gattcaaaat
     5941 gtttcaggaa aaactcttcg tttggattta tgggttgaca aagttattgc tggaacagca
     6001 aacaccccaa gtgataatgt ttatattctc tgcggagaaa tatctcaacc ggtttctgca
     6061 ggtaccggaa ttatctgcga tatcccttat ccaggattgg caatcattgc gaattatcct
     6121 tatgtgaatg gagtaactgg ccaatttagt attcattatt aatgactaag cgaggtaaag
     6181 aatgaaaaaa atgtattttt tggtactttc ctgtttcata gttttcagtg ctaccgcaag
     6241 tggtattctt cacaaatcat ttgctctaat cacttcttca acttatccat ggaatttgcc
     6301 aacacccgga tcaatgacat tgtataaaaa tttatccaaa aatccagtga gctttgtgta
     6361 cactaatgat aaaaactccg atacagttgt tataagatgc cctctaaacg acatagtatt
     6421 acaagccaat tatacttatg tctgttggtt gccagctgga gaaaatgccc aaattgaatt
     6481 aaagccctat tattttcgta atggagcttc tggcagttat tctttaattt tatagattga
     6541 caaaactaag cggcaggaaa ttatacggcc aacaataatc tctccataaa aaactttttt
     6601 tgatagcttg ggcggatatt gagtgatgac gacctgtgtt ataacaagat ctcaaaaaaa
     6661 gataatggtc tataatacaa atatagctag ttgaactctt tgcagagtga aatagaaagt
     6721 aaatcgcaca taagataggt ctatatactt ggctgatttg tttatataga aaaaggaatt
     6781 aaaaatgtcc agaaccggtg aaattcgaaa aatgctagcc actcaaatgg tgcaggcaag
     6841 acttagagaa tattatggcg agtcaatccc ccctatatca gaaagtaatc tcgaggaagt
     6901 atttaatgaa gctcgacagg gaagaagcta ttataatcct cattgggata aatttgatca
     6961 gaaaatcgat atcgaagaac aagccgccat attacttata caggcagttg actcaataga
     7021 cctgcaattt gaggacgaag atacagttca taaggtatta ggtgacatca tagatgaaac
     7081 acaacaatac ctgcgccaaa aatttcccca gggtaaaaat gatctccatg catgcctgaa
     7141 tgattgtaaa aaattactta aaagcaatct tcctaataac gcgatgagcg atcaattcag
     7201 gcaatcattt agatccgagc taaatcaaaa tcccgctgaa agggaagcta tgtggcatag
     7261 taagaaggca gtgaaagatg catatgaaaa gcaccatacc gagcatttaa atcagttacc
     7321 ccttgttgaa aggcaagcaa aaatcaacaa gattattgaa ctcttggatc atagggctct
     7381 ccataatgcc cacaatgaaa aacctaaaat tataaaagat attttatcaa ttgtaaaaaa
     7441 aatgaatccc agggatacaa aaaacatcca ccgtgctttt gaaaaaatcc acggggtaat
     7501 cgaaaattgg gatgccaaag agggggaaca cgaagactct cagtcgctaa tacttaaaaa
     7561 tttagctgtt ttcatttcac agacagctca taccatgcat gaaaatccag aacaaaaaaa
     7621 acagcgtctc gaagaagcca tcaacttatc aagtgaaatt ttaggaaaaa gccctgatga
     7681 aactaaacag attttaagag acaagctaca aaaaagtggg ttttggcata aactggttcc
     7741 aggcgatcct tgggcgaatt gcttaaaaaa ttgcttaaaa ttaactaata tagatccgcc
     7801 taatcttaga aactctccag gcgctttaaa tgcagttaat gcattccgtg gacatgagcc
     7861 tactttaact gatacttacc agaaattatt gcaagtaccc cagctcaaaa ccattgttca
     7921 accacaacca gccaactctt caggagcaaa ggtctcaaca cctgttccac catcacaaca
     7981 aacagccacc agccacagtg cggagagaag aacgcttttg tcagactatc aaattgatga
     8041 tgaaaatacc ttgaaaaaaa ttggacaaga aaaacccggt tttaaacctg ggtaattgat
     8101 tggtccgtat aggattcgaa cctatgaccc tgagattaag agtctcctgc tctaccaact
     8161 gagctaacgg cccatattat aaaaaccact tacaatttaa tagcaacaag tattctatct
     8221 gtcaactgac aaataaatta gaaaatagtc ttatatcatt tatagccatc aagcaaaaag
     8281 ccaatcactg ataacgaaaa tctttgtatg atagtatgat tttaaccgca aatttaattg
     8341 actgaaatgt tgaaactatt tgatgctcat tttcatatca ttgattatcg ttttcctcta
     8401 atcgctaatc aatcctatct tcctcctgaa tttactgttg aagattatct ccaacgtgca
     8461 aagcccctga atatttgtgg aggagccgtg gtttcaggtt catttcaagc ctttgaccaa
     8521 acctatctgc taactgcttt atcggtttta ggcccaaact ttgtgggggt tactcaatta
     8581 cctgccactg taagtgatga agaaattatt caattaaatc aacatggtat tcgagcggtt
     8641 cgtttcaata tcaagcgtgg aggctcagaa agcatccatc agttaaagta ttttgctcat
     8701 cgtatttatg aaatggtaag gtggcatgta gagatttatg tagactcaaa ggagcttggt
     8761 gatttgacta atctgttatt ggaattgcca gctgtcagta ttgatcattt aggattatca
     8821 caatcaggat tttccgagtt actccatctg gtggagcatg gcatcaaaat aaaagcttca
     8881 ggttttggga gagtcgattt tgatgttaaa aaagcactgc aaaccattgc ccaaattaat
     8941 ccagacgctt tgttatttgg tacagattta ccctccacgc gggctcccct cccttttctg
     9001 gatagtgaca ttcaattaat ccaggatcta tttgatgagg agctgacaga aaaaattctg
     9061 tacagaaacg cgtgtaattt ttacagtaaa aacaaacctc ttgcataagc tgattatttt
     9121 gttttacgcc aaggtgaaaa cgtctcagcg agcatagttt agatgcagta aatcagtgcc
     9181 gcagaaatgt ttgcatggca gcattaaaaa gataaagact ggttatgcaa gagctctatt
     9241 ggttcaaagc gaggtttgca ccttttgttc cccagctttc ctgtttctaa gtccatcgtc
     9301 ctcttctgta ggtgaagttg taaatatccc caagctcccg tggctggctg ctgtaaaatc
     9361 aaggcgggct ttttgatatt gtttacaaat ctctttctga agttcaatcg gcatcatacc
     9421 gacagcacca tacactttga cattaccgct tggtttcttt ccagggggat aatttttaac
     9481 atcaatcacc gcctctctta aatctttaat aaattgcgtt tcgaagcctc ttactaaagt
     9541 gtgcacatgg gtcaaacata aatgaaaccc atccggattt tgtagcaagt taagcttcca
     9601 tccccgcttc tccaattcat ctgcaatgaa atgggcattg caagtgttac ttctaaaccc
     9661 cagaatggac cattgaggat taccataaac ataaatatct tcgctggtta atccattgcc
     9721 ttcctccact aatgccgtca attctttttg aatagcattg cgtaatctga taatgctttt
     9781 ggcgatttcc tgatatttat ttttcccata gtaagacaat gtagcgtaga cttccgcgac
     9841 acgagctcca ctggttgaac catccagaat tcctggggta gcatacaatc cacctgacca
     9901 gtttaatgcg gcataaaccg acaaagcagg cgaatcctca ctaaaaagac aaacagaggt
     9961 gccttttgga caacagccat atttatgcaa gtcggctgaa atggaagtca ctccaggcac
    10021 tctaaaatcc ataggctcgg atgaagtgtc taaaaaagcc gttaaaaacc cacccaaaca
    10081 ggcatctaca tgaaaaggaa catttttttt ctttgctaat tgacctaact cagagacagg
    10141 atcatgaatt ccattcatga aagatggggc agatccaact attacggcag tatttctggt
    10201 aatataggaa ctcatgacct tgggattcac cgcacctgtt tttttatcaa caggaaccgt
    10261 aattaatatt gctccagtta attcggctgc ttttttaaat gcagcatgtg ctgtttcagg
    10321 aacgacaatt tcaggataat caatgccttt tgcccttgcg cgaatgacat aagcagccat
    10381 cgcctcaata atgctggttg tacccccatg agttaataaa ccataacctt cttttgaacc
    10441 atgaaacaga ttttgacacc agcgaatcac ctccgcttgc attgcgttaa ttcgaggcca
    10501 tttatcatgc aaaggattag ttagagcagt tgccccataa acttctttaa gcagctctgt
    10561 cagttcctta gggtgaatag cgtataatgc acctgaatct ttaccatccc ctcctccaac
    10621 aagaaactcc ctctcagggc tgtcattagt tacggataaa aaatcaaaat gacatttttc
    10681 cacatccaca tcaaatgcac ttaaaatatc ttggggagaa agcccttcct caggtatttc
    10741 ctccctgaga gtcataccgg aacgctgaat tctcaatttt tctctcgttg cagagagttc
    10801 tttattgagc tctttctcaa tgatctgatt cacgccaggt aaattttttg ccaaggcgta
    10861 agcactatca ataatacgct gcttcatact ggcgttattg cggctacggt accacctgga
    10921 aatcgatggg ttatggtatt gattataaag aaaataaaga gcagctgtgc ctaatatgat
    10981 ttgatgtgcc ggagtatctt gtaataattc atctaaagaa ctgactgctg ctgttaataa
    11041 atctgaaata aaaccgaaca ttgcacatat ccttacgaac aaaatgttgg ataatagtca
    11101 ctttgcataa aaaaataaag accggttgat gcacgccagt ataacaaaaa ttctatggta
    11161 ttttgcttga gccggatgat ctccaaatgc aagatataat attcacggta ttacgcataa
    11221 tacatcttta taagctaaac taagcttatc tactggatca taacttacca gtgacttgaa
    11281 tatcagggat agataacaat gaacaaaatg ttggcattga tcctacaatt actggcaatc
    11341 attcttattc ctgccctcat ttcctgtagt tcagaatcca ataaaccagc agggcaaatt
    11401 ttggcggaag ttgatgtcgc gcttccagta aaaaagaaaa ttgtagaatg ggatgaatac
    11461 actggacgat ttcaagcggt agaggaagtc gatgttcgcg ctcgggtcac tggttatctt
    11521 gaggcgatca agttcaagga tggtcaaacg gtaaaaaaag gagatgtctt attcgttatc
    11581 gatcaacgcc ccttcaaata tgctctggca agagcagaag ctcaatacgc attagccaaa
    11641 gcgcaatatg aacgtgccat aaaacttcaa aaagagaaat ttatttcagc tgaagtgata
    11701 gatcaacgat ttcaagatgt acaagttgct gaaaccagag tgcaggaagc aaaattgaat
    11761 ctggagttta ctgaaatcaa agcccccatc agtggcaaaa taagtcgtga ttttgtgagt
    11821 gtgggtaatt taatccgcat gaacgacacg gtcttgacta aagtagtttc tattgatccc
    11881 atttattttt attttgaaac cagtcaaaat gatttgctga aatacatacg tcttgaacgc
    11941 gcaggaaaaa gacttagttc tgagaaagaa cccactcccg tcttaattaa gttacctgat
    12001 gaaaaggatt acctgcatca gggagaagta gattttttag ataatgtgat tgactcaggc
    12061 acaggaacta ttcttgccag agcacttgtg cctaatcctg ataacgtcat ttatcctggc
    12121 ttgtttgcac gtgtaaaatt agttggcagt gaggaatatg aagcaatttt actccctgat
    12181 aaagcaatca atacggatca agctcgcaaa tttgtatatg tagttgataa tgaaaataaa
    12241 gtgaagcgag tatatgttga attaggacca ctgcgcgaaa gtgggtttta cattattcga
    12301 agtggattga aaggggatga aaaagttatc attcatggaa tccaacgtat tcatggccca
    12361 aaccaggaag ttaagcctgt ggtaattgag gtgactgaaa attaaaccag ttgtgtgttg
    12421 gaatcctgtt cattcgatag aatttaatac tgcataaagc atacaacgtt ttacagtgta
    12481 gccaacacga aaaggattta ctggatgaaa atagctcttt tctttattga taggcctgta
    12541 ttcgcaacag taatctccat catcattgtt atggtggggg tattgtccta tttcaactta
    12601 ccaatagaac aatacccaca agtagttcct cctacgattc aggtacaagc ttcttatcct
    12661 ggagctaatg caaaaacagt agctgaaacc gtggcaaccc ccattgaaca ggaagttaac
    12721 ggggtggaaa acatgctgta tatggactcc caatccactg atgatggaca aatgcgtctg
    12781 accattactt ttaagcttgg aacagatctg gatcaggcgc aggtactggt gcaaaacagg
    12841 gtggcagttg tggaacccaa attacctgaa gaagtccgcc gcttaggaat cactactaac
    12901 aaaaattcac ctgatttgat gttggtagtg aatctgtact cccctaatgg tcaatatgat
    12961 caaacctaca tcggcaatta cgctatcttg catatacgcg ataaaatcag acgaattgaa
    13021 ggtgtaggta atgtactcgt atttggggcc agtgaatatg ccatgcgtct ttggctgaat
    13081 cccgatttaa tgaatacttt taatttaacc gcaaatgatg ttttagcagc tgttcgtgct
    13141 caaaacgtgc aagtcgccag cggcaagctt aatttgcaac cccagaaaaa ccaatacggt
    13201 ttcgagtacc atattgaaac caaaggacgc ctggttaaaa aagaagaatt tgagaatatt
    13261 attgtcaaat caggtgaagg cgggcgtatc gttcgcctga aagacattgg tcgcgtcgag
    13321 ttaggtacgc aaaattacct gacgaaaggt tatttagata aatatccagc tgttgcatta
    13381 cctatttatc aacgcccagg aaccaatgca ttagccaccg cacaagaaat cattaaaacc
    13441 atgaaaacaa ttgccaaggc tttcccgcct ggcattacct atgacattgc ctataatcct
    13501 actctatttg ttcaacaatc aattgatgct gtatttcata ccatttatga agcgattgct
    13561 ctggttgtac tcgtcatttt gatttttcta caaacatgga gagcctctgc cattccagta
    13621 gtagctattc ctgtatcatt gataggaact tttgccgtga tgcaggcgat tggattttca
    13681 ttgaattact tgaccttgtt tggacttgtt cttgctattg gtattgtggt tgatgatgcc
    13741 attgtcgttg tcgaaaatat ggagcgtaat atccaggcag gcatgaatcc ccgcgatgcc
    13801 gccagaaaaa ccatgacaga agtaggatca gcattagttg ctatgggttt ggtgttgatt
    13861 gccgtgtttt tacctaccgt ttttctggaa ggaatttccg ggcgttttta tcaacaattc
    13921 ggtacaacgc ttgctgtcgc aactgccatt tctgtttttg tctcgttaac cctgactcca
    13981 accatggcgg tattattgct tcgcgctcat cacgaaacta taaaacaaga aggattagcc
    14041 tggtggagaa agccaatata tggatttttg catggattta accgactaat ggaaggattt
    14101 tcccggcaat atgggaaatt ggttgctacc ctcacacgta aaacggcttt aatgactatt
    14161 gcttatggta tctttatttc catcacactc cttctatttg tctacgtacc acgcggcttt
    14221 atcccgagac aagaccaagg ctattttatc gttgcagttc aattgccgcc cggagcctct
    14281 ttgagccgta ccgatgctgt catcaacaaa gcggtcaaca aaatattggc gattcctggt
    14341 attgcccata ccgttagttt taccggcttt tctggcgcta catttactaa ttcatcaaat
    14401 gccggagcaa tttttacccc cctactgtca tttcaagaaa ggcaacgtat ggggataaat
    14461 tataatgata ttcttaagcg tctaaggcag gaattaagta ccatcaagga agccctcatc
    14521 gtggttatcc ctcctcctcc agttcgaggt attggaaatg caggtggttt taaaatgatg
    14581 cttcaagaca ggggtggtcg aggtcttgat gtattaatgg aagcagcagc aacaatggtt
    14641 aattcagcga atcaggaaaa agccaccaca tcagtattta ctttttttga aaactcaaca
    14701 ccacgacttc acttgaaact tgacagagaa aaggtggaac gacttaacgt gccatacgcc
    14761 aatgtcgtgg aagcactgga agtttacctt ggctctgttt ttattaacga attcaattac
    14821 ctgggacgta cttttcgtgt tattgcgcaa gctgattcag aatatcggca tactgaagat
    14881 gatattttgc gaatcaaagt caaaagcaac aacggagaca tggttcctat aggctcggtt
    14941 gcgcaaatag aaaatacggt tgctccctca cgtatgcctc gtttcaatct gtatcccgct
    15001 attgatttgc agggtgatgt tgctcccgga tacagttctg atgaagcgct ggtgaccatg
    15061 gaaaaactgg ctcagaataa tcttccagac gggattggtt atgagtggac agaaatagcc
    15121 tatcaacaga aaatggtagg taatactgct ttgatggctt ttactctggg tgttattttt
    15181 attttccttg tccttgctgc tcaatatgaa agctggtctt tacctttagc cgtcatatta
    15241 attgtaccga tgtgtctctt ttcttccatg ctgggagtta aaattctcgg gatggaaaat
    15301 aatattatga ctcaaattgg ttttctggtt ttgattggac ttgcatcgaa aaatgccatc
    15361 ctcattgtgg aatttgcgag acaacttgaa aatcggggct ataacctctg gaaagctgcc
    15421 attcaggcag caaaacttcg tctgcgcccg attttgatga cctcatttgc atttattctc
    15481 ggtgtttttc ccttgattat ctcaacaggg gcaggcgctg aaatgcgccg agcgctcggt
    15541 gtcgctgtat tcagcgggat gttgggagta actttttttg gccttatctt tacgcctttg
    15601 ttttatgttt taattagtcg cttgagtaga tataagcgga aattgaccag gcctgagaaa
    15661 tgaaatcgac ttgcaggagt ccctaagtta tttataaata gtctatagtt taaaaatata
    15721 gactgcattc ttcagaaatt attcttatga ggacatatga attcttcaac ttctggaaaa
    15781 aataatcaac aaaagaatcc tttacccact tatttttcag atccacctct tcaacatgta
    15841 gacttgttca agctttcatc ttacccggtc cttgatgtcg aggaagccat taaattcaca
    15901 ttaactgaag aaaatcttgc cgagatgctt catacgatgc tcaatcactc gttgccggat
    15961 gaagtcgctg aaatgaaaaa atttcatgac ttgggagatt gggaaaaaac ccaagacata
    16021 gcacataaaa ttaaaggcgg atgcgtttat gtgggtacag tgcaaatgaa aatggcttgt
    16081 caatattttg aacgctattg gaagtcagga gagactgact tactagaagc attatatcaa
    16141 caaattctcc atacaattga agaaaatgta cgttatataa aaaactggct ggacaagcgc
    16201 aaataaaata aaaactattt atacaacaga aagctccaat caaaaaagat tatgatttca
    16261 ttttcttttt tctttagagg aggctattgc ttatgcatct ctatctattg cctagtaatc
    16321 accccaaatt tattgtattg ataatccctc tgagacaaat gaatgaaaga atgcatgtaa
    16381 ctgtctcttt aaactattcc atcattcatc tgtttagttg tcttttttgt gtattttttg
    16441 aaccatagac atcttttttg aaaaactgcg atataatttc accaaatttt taattaaggc
    16501 ctcgaaatat ggaattaaaa aagcaggatt ttaattatac agtcattgac ctttttgaag
    16561 cacaagtcaa aaaaaatcca gattttcttg ctcttaaaga caagacaaca gcgtacagct
    16621 acctcgcatt aaatgaaaaa gcaaatcaat ttgctcgaag tttgaaaaaa aaatcagtta
    16681 aagaaggcga ttttgttgcc attttattag atccgggcgt tgaatttttt atttgtatcc
    16741 ttgctatcat aaaacttggt gccatttatg tacctctcga tactcttgca ccacaaacac
    16801 gtttgcatga aatcgttaaa gatgctaatc ctggtattgt gattacccat gatgaatacc
    16861 aacctctcct cgaaggcatt gatactgaaa tttatctaat caaacaactt catttggagt
    16921 cattaagtca atccaagaaa aatattaaaa acacggtcat gccaacatgc cctttgtata
    16981 tgatttatac ttctggatcc accggaaaac ccaaaggagt ggttgttcct aacagagcgg
    17041 tggtaaatct tgccataatt gataatatca tcgcgataca aaaaggcgaa aaagcaggcc
    17101 aattctgcaa cctggctttc gatggcagtg tattcgaaac atgggttgcc cttttaaacg
    17161 gcgcagcatt gcatattatc cccaatgaag ccagaagaaa ccataatcaa ttaaaaaaga
    17221 ttattaaaga attccaaatc aagtacttac tgattccaac cggctatttc catcagctga
    17281 tgaaatcatt tccagaaacc ttagatcttt tacacactat ttcctttggc ggtgagcaag
    17341 tgaatgttca tctgttaaaa aattttattg attatagaaa aaaagcagat atccccgcaa
    17401 aactggttaa cgtatatggc ccaacagagg ctaccgtatt ctcaagttgc aatattattc
    17461 atagcgaaaa agaatataca gatgagcaac tcgcaagtat aggctttaaa acggccaaca
    17521 caaaaaccta tattcttgat gataaccgcc gtcgcacaga ggaaggagaa ctttgggtca
    17581 gtggggtaca cttggctctg cattaccata acagcccgga acaaacagaa gaaaaatttg
    17641 ttaagaatcc tttttcacgc aacgaactct attcaagaat gtatagaacc ggcgataaag
    17701 ttcgtcagtt accttcaggt gaattgttat acttgggtcg cgttgatgat caggtaaaaa
    17761 ttggcggctt caggatccat ttaactgaaa tcgaacaaga gctcatgaat cataaggcca
    17821 taagccttgc tactgtaacc gtagaaattg gtggtggagc acacaaattt cttacagctt
    17881 acctggtttt taaatcaaaa gacaccattg ttgatgctga cgagattaga aattttctga
    17941 gccaaaaact accaccttat atgttacccg ccaaatatgt catggttgaa aaattacctc
    18001 taaccgccgt gggtaaaatt gataaagcca aattagataa aatagctcat accgatttat
    18061 catttcatat cgacacatca tcttcgaact ctattgaaga aacaataaaa aatatctggc
    18121 gacacctttt aaaccgttcc agtattgata ctcacaagaa tctgttcgaa ttaggagcaa
    18181 actcattact cattacagaa gcatgcgctc aaattaataa agaactccag tgtgatttac
    18241 agatagttga catgctgacc cacccgacta tccacaaatt aagtcgcttt ctcgaaggcg
    18301 atttggatac acatcttgca cgaaaaataa aaaaagccgc ttcacaggac attgcaatta
    18361 ttggaatgag ttgccgcttt cccaaggcta gaaatataga tgaattctgg catcatttgt
    18421 gtcaggggaa agattgtctt actcgatttg acgaaaatac tatcccccac catcttttgg
    18481 cccaagaaga ctatgtgcct gtaaaaggaa tcttggaaaa tagcgatcat tttgacgcgc
    18541 atttttttgg cttcagcccc attgatgcca aaattaccga cccgcaacat cgtgtctttc
    18601 ttgagtgcgt atgggaggct cttgagcatg ccggtattgc cccaggcaaa ctggattcta
    18661 aaatcatcag tgtcttctcg ggaatgaccg acagcacgta tctcaatgaa aatttactta
    18721 aaaatcaatg gttttgtaat gagtttgacc aatttcagca aagaatagct acaagcatgg
    18781 gaatgcttag cacccaaact tcctatcggc tcaatttcaa aggccgtagt gttaatatta
    18841 ataccgcctg ctctacaggt ttggctacgg tagatcatgc ctgtcaagac ttaatactgg
    18901 gacaaagtga catagcgctg gctggtgcaa gctccatagt cgttcctgaa acacggggat
    18961 atttttttca aaatggcagt attgtctcac ccgacggata ctgcaggcct ttttcatctg
    19021 aagcaaacgg cactgttttt tccaatggtg ttggtgtcat tgttctaaaa cggttagagg
    19081 atgcaataaa gaatcgcgat acaatctatg ccatcatcaa agggagagga gtaaataacg
    19141 atggaataga caagttgggt ttcactgcac cgagtatgag tggccaaatc tcatgcattc
    19201 gggatgctct tcaacaggcc cagattagtg cagaagaaat cagttttctt gaagcacacg
    19261 gaacagcaac agcattaggt gatgttattg aaatacaagc attaacttct gtctatcgag
    19321 aacaaactga taaaacccag ttttgtgctt taggctcagt caaagcaaat attggacata
    19381 ctgatgtaac agcgggtatt gctggcatta taaaaacagc tctctgcctt tatcataaga
    19441 aaatcccacc tcttattcat tataaaaaac caaaccccga tttatcactg cccgaaagtc
    19501 cctttttcgt caatacggaa actcaagatt gggacgctgc ttcaaaacgc tatgccgcag
    19561 taagttcttt tggaataggc ggcaccaaca cccatatgat tttatccgaa catgaacatg
    19621 aggaaaaaat ggcttctgtt gcccccttga aagaagaatt aatacttctt tcagcaaaaa
    19681 cagaaaaagc tttacaacaa agtacttaca acctcaagca ctacttaaaa aaatgtgatg
    19741 actccaatct tgggaatgtc gcctataccc tctatagagg tagagaagac ttccaatggc
    19801 gccaatttgc tgttggtaaa acagtggaag agatatgtgc cgagctcaaa ggaaaaaaac
    19861 ctttattttt taatcaagat actcaccata gtatcgcatt tatgtttagt gggcagggaa
    19921 tgcaatatac ccaaatggcc atggaattat ttaaaacaat tccattattt gcaaaatacg
    19981 tgacttcagg ggcagagatt gcacaatcct atttgcattg tgatttactt tcaatcctcc
    20041 atgattccag ctcaaagaaa cttaaagaga ctcaatacgc tcagccggct ctatttattg
    20101 tggaatatgc tttggcaaaa ttacttatgg agtgtggcat aataccagag atgctaatcg
    20161 gtcatagcat tggcgaatac gttgcagcgt gccttgccgg ggttttttct tttgaagatg
    20221 gaatcgcatt ggtgtgtgag cgaggattat taatgtcaag gacacctaaa ggtgccatgc
    20281 tgtctctcga tgcaacacaa gaacaactgg atagctacct gaaaatggtt gatgttgata
    20341 tcgccttaca taatgccacc aatcattttg ttttatcggg cagtgaaccc gagattgaga
    20401 aattagaaag ctacctgact gctcataaaa agccatttca aaaattgaaa gtaaaccacg
    20461 ccttccatag ccgttttatg gaatccattg aaatgccttt taaagaaatg tttactaaca
    20521 taaaactctc ggcaccccaa ataccgatta tttccaatgt aactggagat tggctctcag
    20581 caggtgaagc gattaatccg gattattggt atagacacct gcgcgaaacc gtgcaatttt
    20641 gccagggaat caatcgcata ctgcaagata aacatccaat ttttatcgaa gtaggccctg
    20701 gacaaagctt gtatggattt gtgggggaaa tatcacataa aaaggcaaat gctcttttta
    20761 cactccctgg ttgtcaaaag agactatctg acaggattca gttactctca gcatgcggtg
    20821 aactatgggc aaaaggggtt aacattcgtt tagaatccct ggtagacaat gacaaccgaa
    20881 aatttattcc cttgcctact tacccttttc aaagacaaag atattgggtc gatccggata
    20941 aaaaaaacat ttctgtcaat gaagcctgct cgttatataa acccgtatgg tctcatgaaa
    21001 aaatagctca tgttactcct gctaatctta aaaattttga ctatgtcatt ttcaaagata
    21061 aaacaggaat aggtcagaag ataattaata tactaaaaaa tcatgacatc gaacctattg
    21121 ttgttgaatt aagtgaggaa ttcagacaat tatcgaaaac tcatatcagt atcaatccag
    21181 gagaaaaatc agattacaat caattcgttt catcgctaaa aaatacgctg cgtcatccaa
    21241 ttatttttca ttgcttttca tgtgatagct ctgaggatag ctttcttgat acacagcaga
    21301 ttgaaaaccg attaaagctt gggttttata gcctcctcta tctgactcag gcattcttaa
    21361 atgatttggg aaccaacatt ccaattcgct tggcaattat tacttctggc actcaaaatg
    21421 tcattggtac tgaaaagata tctccaatta atggcagcct ggttggcgct tcgcgcgtca
    21481 ttccactgga acaccataat atttcatgtg aactgttcga cattaatttg tatgaaacaa
    21541 cccaaaatat taccaatgcg attatcgaca ttgtcagttc ttctacgcaa gcagcacaag
    21601 atctccccta caaattaact gcctatagaa atagttatcg ctgggaactc tcttattccc
    21661 caaatcacaa caagactgtt ttacaacgtt taacagataa tggcatctat ataatcacag
    21721 gtggcttagg gggtattggg ctatcactgt gccatagcat cttgaagaat accaaatccc
    21781 caacatttat tctttgctca agaacacctg tgatcgatga atcagactgg gaaaaaatag
    21841 ccacagatac ttctcatccc catcaccata aggtattaca actaaaaaaa ttaaaagaac
    21901 ttggagcaac tatcgtttgc tatcaagccc atattggaaa tgataaggat gtcgatcgtt
    21961 ttatcacatt ttgtaaatcc agatttaaaa cgattaacgg tctcattcat gctgcaggag
    22021 ttgctggtgg cggtttgtta caactgaaaa caaaagaaaa cgccgataat attttgttac
    22081 caaaaattca tggcacctat cacttggcaa aagcattacg cgagttccat cttgattttg
    22141 ttgttttcat gtcctcaatc gcatcgatta ctggagagag aggacaaatt gattattgtg
    22201 cagcaaatgc ctgcctggat acttttgcaa acagcaatct attttataca caatgtttgc
    22261 ttagtattaa ctggaacacc tggcgtgatg tagggatgag tgtagaaacc caaaggccgg
    22321 aagatatcga tttttttgct cggggaaata ctattagtcc attacaagga caagagttat
    22381 ttttaaaagc actctctagc aattgctcca atctcattgt gtcgaattat aatattgaag
    22441 actattccag cttgatactt gataataatc ctgctgatac cgaagaacat aaagtttccc
    22501 gcgacacatt tagtatgagt cacgaatttt gtgctccaca gggaagcatt gaaaagcgtc
    22561 ttgcaaaatt atggcaagac aatcttcata ttgtcgatat tgggagaaac gatgattttt
    22621 ttgcattagg aggccattcg ttaaaagcat tacacctcat tgagaacatt aattctattc
    22681 taggggtcaa tttatccatt caacatttat accaagcgcc aaccattgcc aagcttgctg
    22741 aattaatcta ttctaaaaac cccgaaaatc agattgaaat tgtaatacct ctgaaagaaa
    22801 cgagctcaaa gcaacatcct tttttcttct gccatcctgc aagtggcatg gtttattgtt
    22861 ttgatgattt ggcatccttt tgggactact caatctcttt atatggtctt caagatccaa
    22921 gtgtttcctc tggaaaatta atttttgaga gtctttcctc tatggcaaag gcatataccc
    22981 aagccataaa aaaagtacaa ccacatggtc catacttttt ggtaggatat tcgtttggag
    23041 gaagcttgat gcatgaggtg gctcatctac tcactcaaca aaatgagcgc attggattac
    23101 ttgccatgat tgatagctgg tgtactttct caagcaagca aaaatccaaa aatcatttca
    23161 ttgaggccta tctatcaaaa gaattgcagg ctagcagaaa tttggctgat ttggcatggg
    23221 aaagaatgga attgctactc aatcataagc caacaaaaat caaccaagac atggttctat
    23281 ttaaagcaac agatttaaat caagactatt tagatatcga tgatccaaag aatggatggg
    23341 gacaatataa cttagggcaa attatctgcc atactataga agcgaatcac gatacaatca
    23401 ttcgtcaggg atatcaattt attgcgcaaa agttaattga gtacagggac attgaatgag
    23461 atcacaaacc attcgtttga ctcagaaaga ttttcccatt tttggaaaag cattttcatt
    23521 agttaacatt tcatttctcg taatcaacga gaatatggaa ttggaatata ttaatgatac
    23581 tgcaaagaaa aaacttaaaa ttaattggag ttcaaccgat agaaaatctt ttttccaatt
    23641 atgggaatta gccaatctaa ctccaatcct gactaaaaaa ggcgatctta ttcctaacaa
    23701 agttttacaa gtggggagtt attctattca atggcaaaaa acagtcgcaa tagtcaacga
    23761 aaaacattac attttgttaa tggacaaaga tgttgcaaca acctcacatc ttttcgaaga
    23821 aatcggggct gctatctatg gtgaaatagg atacgcacct aaaaacaaaa tttctgtaaa
    23881 agaatatatt caagaattaa ccaactatta cacctccgtt attgataaaa ttccttgtta
    23941 tgtttactgg aaaaacacca gttttgaata catgggttgc aatgaaatgg cagcgaagtt
    24001 ttttggcttt aaatcgacat cagatattat tggaaaaaat gattttgatc tttttcaaga
    24061 tcctgatttc gctaaaaatt atcaggagca agataaacaa gtattttcaa ccggcgaacc
    24121 tattttaaaa atgccatctg atctcaaaga ccgcaatgga atggtcacta atacattagt
    24181 cagtaaagta cccataacta atttatcggg aagcatagta gggcttgtgg gaattactgt
    24241 tgatgtaact gaattgacaa aagcaaaaga agctgctgaa gcagctaacc aagccaaaac
    24301 tgcttttatt gccaatatga gtcatgacat ccgcacacca cttacagggg ttattggaat
    24361 gtctgaatta ttggaaaata cagtgaaaga cccagagcaa aaggaagaag cacacatgct
    24421 tcacgatagc ggtgaagaac tattaaatat gttaaacgat attctggatg atgttcatgc
    24481 tggctttatg gataaagaag atatccgcat agaaactttt gatttgtatc aatgtattga
    24541 agatctggtg aaactcgaac gccctactac agtaatgaaa catttggaat taattgtgca
    24601 tattggtaaa tctgtacctc gttatatcca gagtgatcgt aaaaaaattc atcgagttct
    24661 tttaaattta ttgggcaatg cgattaaatt tacccctaaa ggtcagatta cgatccaagt
    24721 agcatgcctt gaagtttcag gaaaaaacat tcatttgcaa tttaaagtca gcgatacggg
    24781 gataggtatt ccaaccagtc ttcaagaaaa agtgtttgag cgttttttcc gaatcagtcc
    24841 ctcttataaa gggatttata aaggacatgg tcttggctta catatcgcac aatcgtatgt
    24901 caacttatta gggggccata ttgctttaat aagcgaagaa ggcaaaggaa ccacttttta
    24961 tttcgatatt ccatgccaaa caggcacgca gaaaaaaata aaaaccaata agttaacgaa
    25021 cagttcaaaa aagattgact cctcgctccc cacctcatct aactcccaat taactcccaa
    25081 gttgtcatcc aaaactgaag aaaattcgcc ctacgtatta ttagtggaag acaacaacat
    25141 tgcattgaaa gtgctggagc ttattttatc aaatgaaggt tatcgcttta aatctgcttc
    25201 taatggagaa gaagcattgg cattaattca aaccgaatcg tttgaccttg tcatcacaga
    25261 tcttggtctt ccaggaatat ctggttgtga actttctgtt aaaattcgtg aatgggaaaa
    25321 aaaacattcc aaaaaacctc ttcctgttat tggattgact ggacatacta aagattcctc
    25381 tgaaaaagga tgcctcgcgt ctggtatgaa taatgtgttc actaaacctg taactccaga
    25441 gctaattcgc aatataatca ataagtatct attaggagaa aaataaaaga aaaactcggt
    25501 taatttaaaa ttcacttgat tcatataata aataagcaaa ctacccaagc gaacccttct
    25561 aatatagcga attaattaaa gagacaattg gcaaatcaca attgcaattt acactgccct
    25621 ttatggataa ttaaacaaca ttcaggtcta atttttgcta taattatttt aacgtagctc
    25681 caaaagagtt taagtataat cttcaggagg aaatcaatgt cgcaacaaat tggaaatgaa
    25741 ttttttgaag cagatatgga catgcaccaa cattaccttg atatcctaaa ttgcgtaccg
    25801 gatgttgttt actgggtaga taaagattgt cacctgaaag gttgtaatca aaattttatc
    25861 aagttattgg gcttaaaaaa aataggcgat ttaaaaggaa ccccctatga ccaaatggca
    25921 aaacatatga agtggtcaga aaaacgaatt gaaacgttta aactggatga tatgaaagta
    25981 ctcttttcag gtgaatctca gtacgatata gaagaagaac cagtatacaa taaaggtaag
    26041 gaagcaattt actataagtc ccgtcgagta ccattgttcg ataaggaaaa taaagtgtgt
    26101 ggtttagtgg ttgtgttgac tgatatcacc gatctaaaaa aactagaggc acatatacaa
    26161 caaactcatt ctcccaggaa tatcaaacgc actcaaaaga aacgagtgac tgaacccaaa
    26221 atattaatgg tcgaagacaa ttttgttgca caaaaagttg aagaggcatt gcttaaggaa
    26281 ctgcaatgcc aagttgatat cgcggacacg ggagataaag cattaaacct tttcgacccg
    26341 gggaaatatg atatggtctt tatggatatt ggtctcgaag atacttctgg ctatttggta
    26401 gcaaaaaaaa ttcgccaaaa ggaacaaaac accaattatc atgtgccaat tattgcctta
    26461 acatcttacc aggctgatgt agtgaaatac gatgttgagg attattttat ggatggagtg
    26521 ctcactaaac ctttaacaag cgaacaagca gaccagttaa tcaaacactt tgttcataaa
    26581 gaagatatta tggtttcagg cttaaaatca gcaagagaaa attaagatca acttgcttag
    26641 ctgacaatat ctgattttta gtttgttcgc atgaaacaaa atggaacatt gccaacacct
    26701 ggtaaataga gaaaaggttg aattggcaca tacgtacact acaggattaa agtacaacct
    26761 cgtcggcgag gactgtatcc tcataaaaac aagacacatc ccgcataatg tgtgagaata
    26821 gatttcagcc atgccaaaga tatttgtaat cgctggtttc ttgggtatat cttcccttac
    26881 aatgattatt gatactggag aatgaagtga tatgggatcc tgaacgttat gtgcttggaa
    26941 actatattca aaataaaatc aacgatgtat ttctaaaaac aattcacagc aagctgtctg
    27001 gaaatatttt agatattggt tgcggagatg ggcactatac cagcttgttg gctggcaagg
    27061 taaaacatgg ccaaattctg ggaattgata gctctgaaca aatgatcatg cacgccaatc
    27121 aacaatgggc acgtacgggg ctatcttttg aagttcataa cattgaggaa tttcaccaac
    27181 cgcaatcttt tgatttggcg ctctcatttt ggtgtctgca ttggacaaat atccacattt
    27241 cttttccaaa tatttttcat cttctaaaaa gggaaggcaa attgtatgct gtcatgtctt
    27301 ccttttccga tcacagcatt ttgcaaacct ggaaagaatt ggccaaacaa aacctgtata
    27361 gggatttaac agaacaatac atctctccaa tcaatgaaca gtatttttat ggtgtgatta
    27421 atcttctcaa cagattgcct tttaaacgaa ttaaacttga tttaaaaact tgccgtgttc
    27481 atttgccgca cattgattat tataagaatt tacttctaac aatgcctttc ataaaaagag
    27541 ttccttctga aataacagat acactggttg aggacatgtt aaaagcattt caaactatct
    27601 gtgacaggaa atacggtggt caattatatt atgaaactcg ccccatattt ttagaggcta
    27661 tcaaataact caaggtgtta ggaggcaata ccgtgcattt aaattttaat acaactcctg
    27721 gcatgaacat acctatggtc attgcaaacc aggataattt gaaagcaagc cctacccctt
    27781 tgttacattt tatttctgag tatcatgacg aggtaatcca aaagctcaat cagtatggag
    27841 caatagtatt aaggggattt tcctgtcaag aagagaactg cttttcgaaa gcaatcgaat
    27901 tgtgtgcctt aggcacacga tgttcgacaa gcgattatga cttgccaaga actgtactgg
    27961 caaatgaaat ttacacatca agtgacctgc cggctcatat tcccttaccg ctgcatcacg
    28021 aaaaaccccg atcaccaaaa ccacctaatc acatctattt ttgctgtatc atcccgcctc
    28081 aggaaggcgg tggaacaatc tttgccaacg ctgaagaaat atggatagat atgccccaag
    28141 acattcagaa caagattctt gaatacggtg tccaatacaa gcaatttttt catgggcact
    28201 ctgtaaaata tcgtgtatta agaaaaattc tgggaaacca ttgtgctcga agttgggttg
    28261 attattttgg cacagaagac aaaactcaaa tagaacaaaa tttaactcaa aagcaggtag
    28321 tatgggattg gataaatcat gggattgatt tgataatact aaattatttg cccggagcat
    28381 taaagcatcc tctaactgat aaaatagcct ggtttaattc atcagcttat ttgaattatt
    28441 attcgaattt gaattatggt gaattaaaaa acctgcgttc attcaaatat tgggcaagcc
    28501 ggtatcttat cctgaaagat atgcttccca tgatctgtca ttatggtaat ggccaagagt
    28561 tttctgcaaa agaaatatct gaaattaacc aagttattca gcgtcatact cgtgtttttc
    28621 attggcaaaa aggagatttc atgattgtgg ataattttac ttttatgcat ggcaaacaag
    28681 ctcatgttgg agaaagatta ttgtattcat gcatgacggc ttattaatgc aggtttattt
    28741 ttatccgctc cctcacggtc gcagctcgga ctatggttca aaaactgagc cgcgaccgtc
    28801 aaggagcgga tatggtattc ccatacaaaa tcttgctgaa ttctggctat acttaaggta
    28861 aatagacctt ccaggttaat ttgtttatac tcaaattctc tctctgttat ggttgttttt
    28921 attataactc tttgccagac aaataatttt tgtgcgtacc caggcacttt caggagcatt
    28981 gtaagtggta aacgtaacta tgaaaaacaa aaccaaagac cttttgaatg aacccattgc
    29041 catcattggg atgaattgtc aatttcctgg gattgattct gatatagaag atgttagtgc
    29101 cttttatgac atgctgatga aagggcaaac accaatcaaa gatgtgcctg aaaatcgctg
    29161 gaatattgaa gagtactata gtgctgacag aaaaaaagca gataaaatta ttagcaaaaa
    29221 aggcggtttt ttagataaca cccatttatt cgatgctgct tattttaaaa tatcttctgc
    29281 cgaagcgaag caaatagatc ctcaacaacg cctttttctc gaagtggcta ttcgagcttt
    29341 aaatgatgcc aatatcccgc tagactcttt aaaagactct aacagcggag tttattgcgg
    29401 catttccaca cacgaataca gccaactgaa ctataaagac catatcaaat ttaatgccta
    29461 cacacccatt ggaatagcga acagtgctgc cgctggcagg ctctgccatt ttttgaattt
    29521 aaaaggaccc agcatggctg ttgatacagc atgctcttct tcgttcactg cactctatct
    29581 agcggccacc gcattgagaa acctgcaatg tgatatggca attgtaggag gcgtccattt
    29641 gagtctctgt cctgaaagtt ttgtcggatt aaccaaagcc aatatgctgt cagcgacagg
    29701 ccaatgcagc agctttgaca gcaaagcgga cggctttgtt cgtagcgaag gttgcggagt
    29761 ggttattgtt aaacgcctaa gtgacgccat acgagacaat aacaaaatct atgcactcat
    29821 caaaagcatg gttattaatc aaaatggcga tggaacaggc cttgcagccc ccagtactaa
    29881 tgcccaaatt gccatgcacc aagccgcatt ggaacatgcc catttaactg caggtgaaat
    29941 tgattatatt gaaacccacg gaacaggcac aacagtaggt gatcccgtcg aatttaatgc
    30001 gatccaaagc attcaccaag ggcatcactc tcctcaaaaa cccttgatag taggggcact
    30061 gaaaagtaat ataggccata ctatttcatc atcaggcatt gcttctttaa ttaaagtact
    30121 ttgctctttg caaaatgaag cgattccccc caatttacat tattcaaacc cgaacagctt
    30181 gattgatcct gaaagtatcc ctgcgcttct acccgtcgaa gcaattcctt ttctcaaact
    30241 caaaaataaa aaaagatatg tccaggtatc taatttcagc tttacaggca cgaatgtaag
    30301 tgtcattttg gaagaagcgc ctgagttgaa atcacacgag tcaaagcaag acgacaatga
    30361 accaaaatgt tttgttgcct cagctaatag tgaactctca ttgaaacaat tgatgtcacg
    30421 ctacgaacac tatttgaaaa attcttcctc aaccttgggc gacatatgcc ataccttgat
    30481 caattgtaga gaccattaca aatttcgctg cgccatcatt gtaaacgata aaaaagaact
    30541 cattaaaaaa atggaatcga aagactatga aataaaaaag gtagttctgc aaaacaaccc
    30601 cagaacggta ttgaatgatg ccaaagccat ttacgaagct tacttatccg gcattaatat
    30661 cagtactgat gataaaaata ctgcatacaa caaagtggat ttaccgctct actgttttga
    30721 cagaaaaccc tattggcatg aagtcagaaa acacgtcaaa aatgatcatt ggctggattc
    30781 tttatatcaa caaccaatgg aacaacaagt tgcaaccatc aaagcagagc tcgcctctca
    30841 aatcaaagat cttttaaaga aagacaatat agatccgcat caggatttgg aaactttagg
    30901 tttaactacc tccttattgg aatccctgga tcaaatcctt caggatatgt tttccccccg
    30961 atataaggtg ccttctctcc aatccttacg ctacttaacc ctggataaac tggcaaggca
    31021 tttacaaaaa atgatcatgc cacctgtcgt ttaccgacaa ccatcaatta atgtactcaa
    31081 tgttgaacct atcgcaatta taggtatgag ttgccgtttt cccaaggcag ccaatgtgga
    31141 tgaattctta tctttgctgg aaaaaggcga aagtggcatg atcgatatcc ccttagagcg
    31201 ctgggataac gaaaaatttt atgacccgga tgtcgatgcc ttaggaaaac tatacatcaa
    31261 gcaactgggc ttcattgatc atgtcaacca ttttgatgcg gagtttttca atatcagccc
    31321 gcgtgaggct aagctcatgt cacctcaatt gcgtgttttt atggaaacca gttaccatgc
    31381 gctagaagat gccaatttgc cccttgatgc agtcaaagac accaatacag gagtttttgt
    31441 cggagtaggc actaatgaat atccccgagt tttagcttat caaggtgtta ccttggaaga
    31501 tttgaatatt tactttgcga cggggaatgt tttaaatgcg ctggccggcc gtgtagccta
    31561 ttcctttgac tttcatggcc caatacaagc aatagatacc gcatgctcat cctccatgac
    31621 agccatacac aatgcctgcc tgagtctgca atctggtgac tgtaatatgg ctcttgctgg
    31681 cggtgtgaac atcatcctta gcccggattc taacattact ctttcaaaag ccagaatgct
    31741 ctcacctgac agccgctgca aaaccttcag tgaagacgct gatggctatg gtcgcagcga
    31801 agggtgtggt gttgtggttt taaaacgttt aagtgacgcc attaaagaca acgataatat
    31861 tctggcagta atcaaaggca cctccatcaa cagcgacggt aaaagtggtg gtttcactgt
    31921 ccctaatggt gctgcacagg aagaagtcat cctaagcgca ttggcaaaag cgaacctgtc
    31981 acctggtgat attgatttta tcgaagccca tgggactggc acgccattag ctgatcctat
    32041 tgaagtgaat acactgacca agatttttag tgagcatcac aacaaagaaa acccgcttta
    32101 tatcagctca gtaaaaacca atattgggca ctcggaaagc gcatccggtg tagccagtgt
    32161 tatcaaaact gttttaagcc tgaaaaccaa aacaatattt aagcatctga acttcaaaaa
    32221 gcttaatcct gccattcaat taattaatac agtaatccct cttgataaaa ccgattggac
    32281 caagaaacaa ggcttaaggt gtgccggggt tagttctttt ggttttagtg gcgctaatgc
    32341 ccatgccatc ctacaggaag ctcctgaaga caaaaaagaa gcacgcaccc ttcccgaaga
    32401 atctctgctc gttttatcag caaaaagcaa aacctctctg gagttattac tggccagtta
    32461 ccaaaactat ttatctaata cccgggaaga atttgccgat atctgctaca cagcagcaac
    32521 ttgtcgcagt cattttatgt atcgtgtagc aattaaagcg caaaatgcag gccaagcggc
    32581 caatatcata aaaggcaatg gatacactat ttgccatatc accaaagaaa aagaatggac
    32641 tcaacaaccg gacacacttg cagaattgca aacagcctac ctggatggat tccccatcaa
    32701 ctggtctgaa ttttatcgct cccttggcca tcattttgca aaagtaaaat tacccttata
    32761 cgaatttgaa cgtatagaac attggtttgg tgacaaaaac aagttgaaag atgcacctct
    32821 accaaaggac tggtgttttc aaatccagtg gcaaaatgaa ccatgtgata aaaacaatcg
    32881 aaagctgaga ggaaatagat ggcttttaat tggtcccaga catctggctg atggtttcaa
    32941 agctcaaggc ctggaaattg tctacgaaga tgaagagtat tctcgaaaca aactggaagg
    33001 catcattttt gctgaaagtt tgcatctggc accacctaac atagaagcta atattgattt
    33061 tcaaaagaaa accttaaaaa agctgctgaa cttaattaaa gaacttgata accagtcaat
    33121 tgatttgcaa ttgctcattc ttaccagcaa tggaattgct gaactggcga atgacaaaca
    33181 ccctgtcaat ataagcaata gccctcttat tggtttctgc aagactctcg ctttagagct
    33241 gccacaattc aaaactattc tcatcgatat ggataaaatc gaagaggaca aatacccgac
    33301 acaagtgata gaagaaatca attataacca tggaagccgc tatgaacaca tagtagctta
    33361 ccgtggtggg gaaaggttgg cttcccgttt aaaaaagact ccgctggaag ataggcgacg
    33421 actattgcat ggagtgaaag gacgctatct tattgctggc ggctgcggtg gtttgggatt
    33481 ggtaactgcc caatccttgc tttcctctgg agcaagagaa ttaattctca cctcaagaaa
    33541 tatcgataaa ccaaggatca aggaagccat ttataaaatt aaatcccatt accctggcgc
    33601 caacatccgg gttgtcagtt tagatattac tgatagggaa agtttaaaaa atttattatc
    33661 agagcttaat gccgatggtc agttaaaagg catcatccat gccgctggtg ctgcaattaa
    33721 agcgcctttg cttgaacatc aggatgaaga cgttgattat ttattttcag ctaaagtaaa
    33781 aggtggatgg tttttacatg aactaagtct ggattgcaac ctggatttct ttgttgtcta
    33841 ttcttcagtt tcctccgtat ttggcagcaa caaagaatcc gtttatagtg gaacgaacag
    33901 tttcctcgac gccttgatcg cagagcgtca gcgtatgggg ttacctggta ccgctgttca
    33961 gtggggacct tggggtgaag taggaatggc ccaaaaacgc tcgcgtgatg aaggactaaa
    34021 acaagcgtta atcagtaatg cacaaggaca tgtaatcatt aaatgtctca tcaacgacga
    34081 attcaaacac gccactatca tatctccaga atacctcaaa tttatgctgg attttgtacc
    34141 caagccctta cctgggtttt atggacaact tgatgaggag cttgaaaaaa taccacaaca
    34201 acatcatcaa agcccggcaa aaaatctgtc tccatggctt aatcaatatc tggaaattag
    34261 tgaagaggag cgatttaaag cctgtaaagc aatggtatct gatatttgcg caggaatcct
    34321 tgaactgaca gaaaatgaag acttaaacga agacgaaggt ttttttgagt tgggttttga
    34381 ctcgctcatg attactgaat tggccagtga actaaagaaa aagctggaac cattccttaa
    34441 agttaccgtg accattggtt ttaattatcc atccatcagt aagctggcca aatacattga
    34501 atcagagctt gataagaact tggtcaagat acaaagtcca aaatcaactc ccaagccgat
    34561 tgatgacggt atagcaatta taggcatgag ttgtttatta cctaatgcgc cagacatcgc
    34621 tgcctttgaa aaactgttgg aagagggatt aagcggtatc aaagacattc caaatgaccg
    34681 ctgggataat agccaatatt acgatcccga tatggaagcg cctggtaagt cctacgttac
    34741 taaactggga ttaatcgaaa atattaaata ttttgacgct gcattttttg gtattagtcc
    34801 ccgagaagca aaattgatgg agcctcagca aagagtattt cttgagtgtt gctacaaggc
    34861 attggaaaat gccaactacc cttcggaatc tttgcgtggc agcttaacag gcgtatttgc
    34921 gggcgtaggt cccaatgaat attatgcgca actggaaaaa tcagggtttt caaacgaaga
    34981 actcagcgct tactctatca cgggtaatgt actcaatctg attccaggcc gagtagccta
    35041 cacttttgat tttaaaggcc catcaatcag tgttgacacg gcatgctctt cgtcgctggt
    35101 cgcaatccat tacgcctgcc aaagtttaaa aaacaaggaa attgattatg ctcttgcggg
    35161 tggggtgaat atactgctta tgccggaatc taatgtaact ttgtgcaaag caaaggcatt
    35221 atcacctgat ggccaatgca aaacctttga tgaaagggct gatggatacg tcagaggcga
    35281 gggttgcggc gtagtattgc ttaaaaggct ctctgatgcc ttgcgggata acgatcacat
    35341 cctcgccatt attaaaggct cagccgttaa taacgatggt aaaacagctg gattaaccgt
    35401 gcctaatggt aaaagccagg aagaagtcat gaaaaaagcg cttggtcaga ctgaattgtc
    35461 gagaaccgac atcagttata ttgaggctca tggcacaggc actccattag gggatcctat
    35521 tgaagtggat gctatcaaca aaatctacgg tcatcaacgc aacaaagaga atcccttata
    35581 cctgggtact gttaaaacaa atattggaca tcttgaaagc gcatcaggcg tagctggcgt
    35641 tataaaaact gttatcagcc tgcaaagaaa aaccatttat aaaaatttga attttaataa
    35701 attaaatccc aatatcaaac tggatggtac tcaactggct ttagaaaaaa caaattggaa
    35761 gacgaataca aaactcaaat acgcagcagt caatgcgttt ggttttagcg gtaccaatgc
    35821 acatgttatc cttgcggagt ttcctaaaga agaaaagcca aaaacttcaa aaccctctca
    35881 aaaacatttg ctggttattt cagcctattc caaaacggct ttagataatt taactctacg
    35941 ataccaacag tatttagaga cgactgaaca tcattttggt gacatatgct ttaccgctgc
    36001 gacttgtcga gaacattatc attaccgttt ggctttaaca gctgagaatg cgattgaagc
    36061 gagtcgtatg ctgggtgcag ggcaatttgc cttgtcttat catgaaaata ttacactgga
    36121 cttacaaaac gacagagcgc ttaattccct gttaatggat tatctgcaag gaaaaccagt
    36181 tgattggaaa tcctactatc aatcccttaa aggagagttt acaaaaattc tgctgcctaa
    36241 ttataccttt gaccgcagcg agtattggct ggataaaaaa aacaacgata gcatagccac
    36301 tcataaaggt gagcaacacc ctcttttagg gcaaatgctt accatgccag gaaatgaata
    36361 tctgtttcat cagaaactgg atttggagaa tttggcttat atcaaacaac acagtgtttt
    36421 tgaaaaagta atttttccag caacagccta tattgaatcg ggtttggcag cagcaaaatc
    36481 catatttcaa tccagcgctt ttcaaattga aaaattcaat atcgaacgtc ctctacatcc
    36541 taaacaaggt caggagtttc aattacaagt caaacctaaa atggatggac aatacaaaat
    36601 caatcttttc gcgaaacaag agaacgactg gcaaactttc tgtgaaatgg aaattcatgc
    36661 taagccgaca gcagcccggg aatctatcca tattgacgtg cttaaatcat cgtttggcaa
    36721 taaagtagag ctgtcggaca tatatgagca atttcagaaa agctcattat tttacggcga
    36781 tgaatttcgt gtcctgcaag agggctatgt tcgggcgaac agtgttttgg ctaaagtcac
    36841 attgactaag gcaagccagg gccaggacta ttattatcat cctgttctgc ttgatggtgc
    36901 catgcaaagt atcttgttat taagtgttaa aaatgacgag aataaaaatt ttaacgccga
    36961 gaacgttacc tacataccct atgccttcat ccgcatgact gtatttaagg aagcgccacg
    37021 aagcctatgg gtgcatttaa ctaaacaaaa tactgaagat gcgagtgatc tttgcgttaa
    37081 catcaattta tatgagcaat ctggtttatt aattgctcaa attgcaggcc ttaaattgcg
    37141 aaaggtaaca cgaagtcatt ttatttctta tgactttgtt ttaaaccacc tttattacac
    37201 agaatggagc cctgtccctt taaaaacctt accccaacaa aaaattcctg acctcttggc
    37261 cattacaaaa aatcctggca aagctaaaga aatattggat ggccttaatt atcagctggt
    37321 tgaaaggatc gatgactgca caaatatcga gcacagcaac atagtattcc tttatgaaca
    37381 aggacagttt catgatcttt ttcattgctg ccagatctta tttcgattgc gtcccaagcg
    37441 ctttatactg gttacggaac atgcttatgc cattcaaaac catgatcagg tcaatcctta
    37501 ccatacaatg gcctgttctt tttggaaaag ctttagaaat gaatatgagc ccaataaaaa
    37561 ttatgccatt gatttagact ccaagagtcg cttggctacg gcgttaatgt atttattgaa
    37621 tacagacacc catgacactc aaatagcagt cagagaatct cttttcttac ccaggctaaa
    37681 gaaaaagcaa ttggctagta actctgcccc agagatatta attgatggca atgccactta
    37741 tcttattaca ggcggtactg gaggcttagc caaacccttg atggaatacc tcatgagacg
    37801 cggtgccagg cacattgcca ttaccagccg caccgactat tcggatgaca ttaaggcatt
    37861 aatagataag gccaaacaaa atcagattga tatcaaacat tacatggttg atgccggaaa
    37921 ttatcagaaa atggagcaag tgattggaga aatacaacag ggccccaacc cattgaaagg
    37981 cgtatttcat ttggcgggtt tgatacaaga tgggctcatc gtcaatttga atgatgaagc
    38041 gatgcaaaaa gtaatcagcg cgaagatgga tggcgcttta aatttacatc aattaaccca
    38101 aaatatccca ttagaatggt tcgtcatgtt ttcttcatcc gcatccttgt taggtgccag
    38161 gggtcaggca aattatgccg cagctaatgg atttttagat ggtttagccc atttaaggcg
    38221 ccaacagggc ttaccggcaa tttctatcaa ctggggacct ttccatacca caggaatggc
    38281 agccaatttg actcatactc tacagcatta tggtttttta ccattggata aggacaacat
    38341 tgatattctt gatgtgcttt tgcctagtca attgcctcaa atttctcctt gccctatgaa
    38401 ttgggatgtt tactgcaaac atacaccgaa acaaacggaa ttatccgcgc tggttaaggc
    38461 gaaaccacta cctgatcagt cttttttgaa tgcacttcga caacactcca aagaagaaag
    38521 catctcaata ttgtcacaag cattacgaga aattgcagcg gatgtcctgg ctttggatga
    38581 cagcgaacac atttcgaacc accaagattt gttttcaatg ggtctggatt cactgatgtc
    38641 aatggatatc agaaatcgca tttatgacaa attacaatgc caaacactaa gcttaccgat
    38701 tgaatatttt attaatactc ctaccattaa taaaatcgcc agacatattt ctgatgaatt
    38761 agaccatgtt tttgatagtc actctgacta ccaccctact gaaaatccgt cagaaagaga
    38821 aattgcatta tgtgattttc aatatgtatt ctgggtgctt aacaaattgg attaccattt
    38881 taatatcggt acacaaatac aattacacgg ccgaattaat aaagactatg tccaacgggc
    38941 ttttgaattt gttgtaaatc aaaatagtgt cttctggatc cattttaata aggataaacc
    39001 aacccaaaaa ttgcataaag ttggacaatt tgaattaatt taccaggata tttctctgaa
    39061 tcatgaaact aaagcgctga atcacgaatt ccaaaacaat atattgcgcg ctattccact
    39121 caccaaacag ccattgatta gagtgtattt gtataagatt aataatgaat tgcatgaatt
    39181 acacatcgta ataccccata ttattgtcga tgacgcatca tgcgaaatgg ttttttgcca
    39241 atttaaaaac aattatgaaa aactaactct tgggaaagaa ttagtatcca acccagaaaa
    39301 aggctcttat tttaattatg tgaaacaaaa taataatcac tatcaaaaaa acctgaaaaa
    39361 taaaattgat ttttggcaaa actataataa aggctttaaa atgctgagtt tcggcaatag
    39421 atatcattta tccgatacct caaaacaaac aaagcactta tttcattacc ctattgatcc
    39481 tcaacacgcc gaacaattta taaattggca taaaacccaa aatatcaatg tcagtactgg
    39541 attgattgcc gcatgccaaa ttgttttcta caaactaagc caccaaaata aaataccgat
    39601 cattttaatt cacagtggta gagaaggtgg agaatataaa tcaaccctgg gtttgttctc
    39661 ggaatataaa aggataaatc ttaccttaaa tgagaatagc caatttattg attgtattca
    39721 ttcaattgaa gagcaactct taaaaacagc cccctatcaa aaatgctctc atctcatcaa
    39781 agacagtggc ttgaaaggtt cgagcttatc ctttggtcaa tatttgactt tggcgttaaa
    39841 taagatgttt atgctgaaac actttaaaga aagtaagctc cattccatta tcattgacta
    39901 ttacctgaaa tatttaagtc gcctgctatc cttcaaaaaa aacatttcaa taaaacaacg
    39961 actaaatcaa ctatttaaat tggatatgtc attacaaaaa ccggaaagat taagaattct
    40021 tgtcagcgta acccccagct tttttaccaa agaacgccct gatatgagtt ttgctaatct
    40081 tgattacagc tatcctaatc attttggatg tatggatagg ccgataggaa atcgaacgct
    40141 ttggatttat ttctccaaaa atcaggatgg tgaatatcaa atttccatta atgcgccgtt
    40201 aacaatagaa tgtaaaaata agatcggaag tagtcttagc cagtttatta caacttttgt
    40261 agagaataat aattgtaata ttgctgaatt aattcaagca atggaggatt cctccattgc
    40321 ttcctgattt tactgagcct ggttaaccgt agtattatta ttttttattt gtttagggtt
    40381 tgatgatgaa ggctgagttg aaactttggg taccacaata tccgttccaa acttgccttt
    40441 tatcttgacc tcacctggct gcaatacagc agattggcca ttggctctac gacaacgctg
    40501 ggtatgacat ttcatcttac aaactccctg gacacatcca taacatcccc agttatccca
    40561 acagcaagcc caattgcctt cgattgttga agtaaaagtt ccccctgcca actggcagac
    40621 atctttcatt tcagcatttg atgctcccgt attcggcgca gcataaacat gaattgatac
    40681 aaggctagag agaaagagga aaagcagccc tgccatcctt aaagatttca tgattcacct
    40741 ccttggaatc gtactatcag cacctgcgaa aaaacccaat tgctttgtta aaaattgctt
    40801 tcaatttgtt catttagttt atctaaactc cctcattaaa aacatttttt tgccttgacc
    40861 ttggggcttt tcgtaagccc taactattta attatggcaa aaccccacga ttgttcaagt
    40921 ttcaaacaaa aagaagtctt gaaacactga aaagcctccc ctcaggttac ttcatttttt
    40981 ctatgccaaa tactgaatag aatacaaaaa accaaagttg ccataacaat acctagagca
    41041 atgaaaatcg gtatcttgaa ccaaggtgcg ataagcatct taaagcccac aaatactaaa
    41101 ataaaagcaa gcccatattt gagaaaataa aatcgtttgt gcatattcac aagcagaaaa
    41161 tataaagcgc gtaatcctaa aattgcaaat atattagaag taaatacgat aaaaggatca
    41221 ttggttatgg caaaaatcgc tggaatgcta tccacagcga aaatcagatc actgatttca
    41281 ataagaacca acaccataaa aagaggcgtt acgtagacaa gttgattttt cctgagaaaa
    41341 aatcgctcac catgcaactc ctgggtaatt cgcaaatggc ttcgcatcca atgtagtatg
    41401 gggttttttg cgagctctgg tttcttatca gcaaaaatga acatcttgat tcccgttatc
    41461 atcagaaaaa atccaaaaat ataaagaatc caatgaaatt gattcactat ccatatccct
    41521 aataaaataa gaatcaggcg cataacgata gccccaatca caccatagat tagaacacga
    41581 tgttgatatt tggccggtat tgcaaaatac tcaaagagaa taagaaaaac aaaaatatta
    41641 tcaaacgata aagacttctc tatcagataa cccgtaaaaa attcaagggc tttttcattg
    41701 gccactgaaa aagtaaaatt ctcaacaaga taccaccata gaagaaagtt gaaaataata
    41761 gccagagtga accacaatat ggtccaggat aaagcttctt ttgtggaaac gcgatgagct
    41821 tttcttcccc caaacacaaa caaatcaaca aacagcataa tgagcacaaa tgctaaaaaa
    41881 cctaaccaca tccaccattc actgattgaa gtcattaatt tctaccatgt cttttctatg
    41941 gtttttatac cacattgata tgttttcatt atagtcatag tattagaaat tatgcgcttc
    42001 aaaaaatatt tatattcatt tgaaaaaacc taggaaggag gagaagaaga agaaggatcc
    42061 aaagtagcga tcaattgaat aaccccaaac tcaccattgg ttttcctgcc tacagttaat
    42121 tgaatattca gtaattgaga aacttgcatg gtatcgtttt tataagtgac ctttaagggc
    42181 aatgctataa tccaatggtt ctctctggtt tcaatgaact ctgctacccc attaacttca
    42241 ctagtcatag tgagtctttg cattttaatt gcctcgatat tccctgattt catcatagca
    42301 gaattaaact caatccatcc gttttctgta taacatgatt gtaatttatt tagctgcgac
    42361 tcaatagaag taaaatcaaa agcaaaagat tgtgttgcgg catgctcagc ccattgtaaa
    42421 ataatatttt tatctgtttt tatagtttct tcagggattt tatagtgaca agaaaccgtt
    42481 tcttttggct ttgattgctc cggttgtttg agttgaatgt tttgagagcc gaggaacaat
    42541 tgaggtgata gctgaagaga agatgaggcc agtatcgatt ttaaagattg taacaccctg
    42601 ttagagtcaa ggctcttatt ctttaccgtc aagtgattta tcatcttttg atctaaagct
    42661 aaattaacat caaggcgttg aattattctc tcattgtctt tttgataaac aacttttata
    42721 ggtaatttaa catcccattg attttcttta acctctataa actggggctc tccttctatc
    42781 tgagcactta aaatcggctt ttgcttattg attgcctcaa tattatctga tttgtccctt
    42841 atggagagtt tatattgaga aaaagcacgc cataaaggca aggggttaat tcgagtaatc
    42901 aatgaattaa gttgtaattt agaagaatgc aaaccgatct ggcttaacca gtttggatag
    42961 agttttcgac ttttgttgcc aaaatcactc agagtgctct tgttagaatc agaatcgtta
    43021 acaaaggatc tctgttggga taaagcaaga tatggagttt ttgcagattc aggcatatca
    43081 aatgattgca ttctcacaag attatgagtg ggatttaagt ttggcattgg ttgaataatt
    43141 gccactcttt gcgaaaccct gttactcaaa gcaagatttc ttttagaata ttctccatga
    43201 tttgaaaata aaggcacaaa aaaggaatct gttatttttc ttgcactatc taaactggcc
    43261 ttttttctct cagccagtat tatgctcata cttttaatta cctcatcagc tggcgtcacc
    43321 ttctgagcta gtggcgccag acgaggaata gcaatcattt gcataatccc caaactatct
    43381 ttatttttcc agcctatggt cagataggca ttaagaaaat gtgtcactct ttttttatca
    43441 ttctcataaa caacttttaa gggcaatata attttccatc gattatccaa aacctctatt
    43501 atttgagcct ccccatcttt atagcccttt acttttaatt tttctgcttt aatggtttct
    43561 atattatttg atttatgtaa agcattttgc atttgccccc accccttttc agtatagcaa
    43621 gagtgcaatt gatttaattg gtcctcaata gacgcaaaat tgaaattaaa agacagaata
    43681 gccgcatatt cagcccaatt taaaatgata tccgaatcaa tatgagaaat ttcaggtgag
    43741 attttataat cacaatcaat aacattgtga tagacaatac cctttttttc aatggattcg
    43801 gttcgctttg caatatctcc gctaacagca tcctgagagt aggctttcat actcaataaa
    43861 ataagaaata caccatagaa gagatgtttt ggcataaaaa gattccttat tctgtatcta
    43921 caaatccctt acgaagtgca aatttatcgg ataatttaat ttattctaac gttagttacg
    43981 gataaaatcg gtcactatct tgtatcgttc gggctgagga ggcatttatg ccgtctcgaa
    44041 gccttgtatg gaatttaatg tttcttgcca aggcttcgag acgatgctta cccatctcct
    44101 cagcccaaac gggccttgga tatcgactgc ttcttatata attcttatcc ataactcacg
    44161 ttattctact atttaatagt gtggtggtaa ctcattaatt tttcaaatac tttagtattc
    44221 taatttcgac ccttggaaag aattgctatt cagcatgtgt gttcttttta tactataaaa
    44281 atctgttatt ttgctacttg aaattgtatc aatcgaccct atttaaattt acaactaaat
    44341 agggttttac acctcaattg tgagacgtgg agcagccata aggtgctcgg gtaatattaa
    44401 tcttggcctc aaagttaagg agatatatta tgaacactac ctcattatca ctaaccccgt
    44461 tactacggca ttccgttggt tttgaacgat tcaatgattt gtttgaatcg atgcgaaatg
    44521 ctgatgattc aacctatgcc tatccggcct atgacattga gaaacatgga gaagacaatt
    44581 acatgatcac aatggctgtt ccaggatttc aggagagtga tctgaacatc atggtgcaaa
    44641 atgatcagtt aagagttagc ggtcgcattc aggaaaaaga aaccaaggag tctgaatact
    44701 tacaccgagg cattgtgacg cgagcctttg aacagacctt tcgacttgca gatcacatga
    44761 aagtaacagg tgccgaaata aaaaatggac tattgtctat cagcttgatt cgtgaaatac
    44821 cggaagaagc gaaaccacgc atcattccaa ttaagagtat ttctgatcaa gaaagcaata
    44881 aaaagagtaa aaccattgaa ccagaaagca agaaaaaaat ttccaattaa aaaatgtgcc
    44941 gggtgatatg aaaaatccgg cacagatttg agataaaata gccagaatac ttctgcacat
    45001 attctcttta ataacttttt acaataaaaa taccagataa actatacttt caatttagaa
    45061 aacacattag tattacagag gctcaagcaa acaactgaat atgaggccat tatgaagcgt
    45121 ggtttatttg tccatctcaa acatgactta ccagcatcga ttgttgtttt ttttgtggct
    45181 ttacctttat gtctgggcat agcattggct tctcatgttc ctcttttttc aggagttatt
    45241 gctggtgtta ttggtggagt agtcgtaggg tttatcagcg gctcagcgct tggagtgagc
    45301 gggccagcag caggacttgt agcaatagtc atttcctctg tagagatatt aggcagctgg
    45361 gaagcttttt tgttagctgg tgtgattgct ggaatattgc aattaattgc aggttttctg
    45421 cgaggtggaa ttattgctta ctattttccc tcctccgtaa tcaaaggcat gctcacggga
    45481 atcggtatca ttattgtttt aaaacaaatt cctcacttgt taggacacga cgtcacgcca
    45541 cttctattga gtatgtcgga tggtgaattg gatattaatg acatcagaga agcattggat
    45601 gatattaatc ctgaggttgt tattatcagc attgtttctc ttttaaccct gattttttgg
    45661 gagaaagtgc tggtaaaaaa acacaaacta tttgaaatga tacaaggccc ccttgtcgtg
    45721 gtcatcctgg gaattgcttt gaacttgttt tatgaacacc gactaacaag tttttccctt
    45781 gatccatcgg aactggttca actgccgaaa tttaacaaag catccgaatt atttaattat
    45841 ttaacagtgc ctgatttttc gctaattact catttgaaaa tatacaaaat agcaatcctc
    45901 attgccctaa tcgccagttt ggaaacactg ctctgcgtag aagctgttga taaactggat
    45961 ccctacaaga gagtaacgtc tacagaccgt gaattaaaag cccagggggt aggaaatata
    46021 ctctcttgct tgattggcgg actaccgatt acacaggtta ttgtgcgtag tagtgccaat
    46081 gtcgcttttg gagcaaaatc caagctgtcc gccattttac atggcttatt actttttatt
    46141 tgtgtcatca gcataccaga aatactcaat gaaattccac ttgcatcatt agccagtatt
    46201 ttgattatta taggctataa gttagccaag ccggctttat tcaaacaaat gtataaaatg
    46261 ggatgggaac agtttgtacc atttcttatt acaattattg gaattatttt tagtgacttg
    46321 cttttcggga ttactttagg cttttcagta gctattttta ttatcttgcg ccatcacttt
    46381 cttaattcac atgatattat taaaatccgt ggcaaaaaca aaactcaata ctgtctcaaa
    46441 ctggcggaag aggtaacttt tttaaataaa ggaagtataa tcaacgaatt aaagaatata
    46501 ccagaaggtg cagaggtaat tattgatggg tctcgatcca aaattattga tcatgatatt
    46561 aaagaagtct ttcaaaattt cattttgaat tcaaaaacta aaaatattca agttaaatta
    46621 ataggaattt aaattatgtg gactttaacc aaagaacaac aacaagcaat tactccagag
    46681 aaagctattg agctcttaaa agaaggcaat aagcgtttcg ttagtaacct taagttaaat
    46741 cgcaacttga tccagcaagt caatgaaaca tcccaggggc aatttccatt tgctgttatt
    46801 ttaagctgta tggattcacg cactccagca gagctgattt ttgaccaagg tcttggtgat
    46861 attttcagta ttcgcgttgc aggtaatatt ttaaatgatg atatcctggg cagcattgaa
    46921 tttgcatgtc aggtagtagg tgtcaagctg attgcagttg tcggccatac tcagtgtggg
    46981 gcaattaaag gagcctgtga tggtgtaaaa ttgggcaacc tgacgaacct actgaataaa
    47041 atcaacccag tgattcagga agccaaaaag ttggatgcaa agcacgatgt tcatagccca
    47101 gagtttttga attgcgttac gagtctgaat gtaaaacata ccatgaatga aattacccaa
    47161 cgcagtgata tcgtacatca attgcttaat gaaaagagaa ttgcgattgt aggtggttta
    47221 tatcagcttg agacaggaga agttcaattt tttgatgagt gatatcatga tattaattac
    47281 tttcaataat tagtcttttc aactgtgcaa catttctgat acctaaagct tttaccagga
    47341 attgatgcgg gcttggtg
//
LOCUS       DBNCWK010000011        46479 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.011, whole genome shotgun sequence.
ACCESSION   DBNCWK010000011 DBNCWK010000000
VERSION     DBNCWK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 46479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46479
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.011"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            178..915
                     /gene="pssA"
                     /locus_tag="AC06IO_000513"
     CDS             178..915
                     /gene="pssA"
                     /locus_tag="AC06IO_000513"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIO9873728.1"
                     /translation="MNKENHSGIYLLPNLLTTVSLFAAFYSIVASTKGQYEAAIVAMF
                     IGMIADSLDGRIARLTNTQTEFGAQYDSLSDMVTFGVAPSLLLYNLTLHQLGKFGWLV
                     AFIYTAAVALRLARFNTQIGKSDKRYFQGLPCPPAAAVMASFAWICFQNEWANLIVSL
                     FTAVLALIVSVLMVSNLRYYSFKEVDYKGKVPFLYVLVMILLFVAIAANPAIVLFASS
                     GIYALSGPMLTVISLHKMRKQRRNMSK"
     gene            complement(1156..1425)
                     /locus_tag="AC06IO_000514"
     CDS             complement(1156..1425)
                     /locus_tag="AC06IO_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIO9873729.1"
                     /translation="MIKTKIKIINRLGLHARASAKFVSTAAKFQSHIDVTKDTQTVNG
                     KSIMGVMMLAANKGSELTLEIDGPDEERMNEALVELINNRFGESE"
     gene            complement(1616..1915)
                     /gene="hpf"
                     /locus_tag="AC06IO_000515"
     CDS             complement(1616..1915)
                     /gene="hpf"
                     /locus_tag="AC06IO_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HIO9873730.1"
                     /translation="MQINITGHGMDVTPALRAFTEEKFDKLERHFDHIRAINVVFDVE
                     KVRQIAEATVLVTKGELHASSESEDMYAAIDTLVDKLNRQLIKHKEKLLAHRDRD"
     gene            complement(1942..3336)
                     /locus_tag="AC06IO_000516"
     CDS             complement(1942..3336)
                     /locus_tag="AC06IO_000516"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIO9873731.1"
                     /translation="MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESN
                     PLLEATPVEEKEDSQINTNQNHDEYSDFQWSQLYNNLNKRNNFEENDYNFDNLHCTTT
                     NLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
                     IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNY
                     RQLMKNHQISEKTIHEILQVIHKLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLN
                     QDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQSRQETLLKVATCIM
                     EYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSH
                     VNTDSGGECSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREA
                     MGIPPSNERKVISS"
     gene            complement(3546..3710)
                     /gene="rpmG"
                     /locus_tag="AC06IO_000517"
     CDS             complement(3546..3710)
                     /gene="rpmG"
                     /locus_tag="AC06IO_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIO9873732.1"
                     /translation="MAAVTIKVKMESTAGTGYYKTTTKNPRNHPEKMELMMYDPKVRK
                     HVLFKEKKVK"
     gene            complement(3725..3961)
                     /gene="rpmB"
                     /locus_tag="AC06IO_000518"
     CDS             complement(3725..3961)
                     /gene="rpmB"
                     /locus_tag="AC06IO_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIO9873733.1"
                     /translation="MSRVCQVTGKRPMVGHKVSHANNKTKRRFLPNIQNHKFWVEEEN
                     RFVTLRLSTKGMRIIDKLGIKAVLDKIRAKGEKI"
     gene            4230..4907
                     /gene="trmB"
                     /locus_tag="AC06IO_000519"
     CDS             4230..4907
                     /gene="trmB"
                     /locus_tag="AC06IO_000519"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIO9873734.1"
                     /translation="MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPTPWNFAK
                     EFGRHDADTIVEIGFGMGTSLFAMAMNNPQCNYLGIEVHKAGVGSLVADLHEYQISNV
                     RVVVHDAVEVLQTKIPENSLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSG
                     FIHCATDWEDYAEHILNVLSSESALFNQQKEGGYSPRPDSRPLTKFELRGERLGHGVW
                     DLVFIKK"
     gene            4919..6043
                     /locus_tag="AC06IO_000520"
     CDS             4919..6043
                     /locus_tag="AC06IO_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIO9873735.1"
                     /translation="MIKHAILVSGLIVSFLASAAEMSFLAQLTEVPGASGYEKNVRHL
                     VQKQWQPYMAELKIDGMGNLIGQRRDNQKGPKLLFMAHLDETGFMVESITPDGFLKVI
                     PLGGIANSVIYAQRWTVSTPKGSVLAYSGMDSPHLLGDKKMLGSPDINALFLDIGAEN
                     KEQAQKEFAIQPGLEVTPVSEFSQLSKNRFLGKALDDRLGLAVISDVLRAVKQQPNQL
                     YLAATVQEEVGMRGASTVYKATHPDIAINVEVGIADDYPMLLADRKGRIGLGKGPSLF
                     VYDKSMIPNQELINWIMELANKNNIPLQLEVEPGYGEDGSKVQASGMGVPVVNIGIPI
                     RYAHQHAGIFDKRDYDNTVKLLSLIAENLNQEVVDKIKKS"
     gene            6121..7608
                     /gene="ankC"
                     /locus_tag="AC06IO_000521"
     CDS             6121..7608
                     /gene="ankC"
                     /locus_tag="AC06IO_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkC/LegA12"
                     /protein_id="HIO9873736.1"
                     /translation="MDFVSEMNKILQMELEQFKEFIQKKLEEDKNYLEKLFWLPNGSQ
                     MTVLNYLIEQYSEPKLGIDDANRDLLARIDFVLHEAKDVNVGEPLHQAIVAGKISLAL
                     HLLGVDENNFTSGESEKTDVLSILSKVRKKIEESFNHVKRCFFDVDKRDGYGRTLLSL
                     ALDAKRQELLIAILARNPIVHATTLRSSAYVPFQPIHQAVVLDYAEGITLLASMGAQL
                     TNPLGSMRDTPVILAARLGKINALAALLELPTQSLSLESENNHLFEDKQTGHTAVEEL
                     CERMANENDKADALRGIAMLICRGAEPPRNEKMRNLLSSNRVAFLKAVSTYLGDKPQL
                     VDAFVERCHLRESALHNIVYADHSWGSSIRHLLGVPSEAALIVEELVTRKYSDPSFAS
                     ENVSSLSTTATENLRSERNPLKLYAEFVRRYTQAYDSQMITNRWSTMRWMIAEGNCDW
                     DTVVRYSKNHPTSRTRIVLNEMFNPIPRVHEDIESQQNSPRVVLK"
     gene            7817..8959
                     /gene="hflK"
                     /locus_tag="AC06IO_000522"
     CDS             7817..8959
                     /gene="hflK"
                     /locus_tag="AC06IO_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIO9873737.1"
                     /translation="MGWNEPEKGKDPWSGKNQPPDLDEALKRIQDKLKKTFFGGTGKS
                     NNNSSGGSSGGLLAVTVLLIAFILWALSGIFIVDPAEQAVILRFGKYAETVGPGPHWI
                     PRFISSKIVMNVDRVLDYSYSAQMLTSDENLVSVSLAVQYRINDLSEYLFNVANPEES
                     LQQATSSALRQVVGTTTLDQIITEGREVWGGRVQETLTKTLESYKTGILIVNVSPQPA
                     RAPESVQDAFDDAIKAQEDEKRFKEQAYAYAAKVVPIAEGKASRIQQEAEAYSKQVVL
                     RAQGEVAEFLALLPQYNAAPQVTAKRMYLEAMQKVMNKSSTIIVDSKAGNLLYLPLDK
                     LLGNQYVPQSENLKAIKNGTNVSNEEDNLILSGRDLTRPTYSQGRD"
     gene            8962..9876
                     /gene="hflC"
                     /locus_tag="AC06IO_000523"
     CDS             8962..9876
                     /gene="hflC"
                     /locus_tag="AC06IO_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIO9873738.1"
                     /translation="MNAFKTTLGVLIFLVLVLLFTSMFTVTQGQQGIILRLGRLVKDP
                     QTDAVKVLNPGLHFKTPFIESVRIFDTRIQTMDIKSTRIVTKEKKDVMVDYYVKWRIS
                     DLAQYFKSTGGNEFKAETLLEQQLNTLLRAQFGKRTISDAVSGGRDDVMEILRNAAEK
                     QAGELGIKVVDVRIKGIELPSNTSNAIYQRMRADMQKIANRHRADGQAAAEQIQAKAD
                     ADVTVLLAKTKSNAQRIRAVGEAEAAAIYSKAYTQNPDFFALYKSLLAYEASFHSKKD
                     ILILDQSSSFFDYFKQAMPKNDGTAAKK"
     gene            10011..11306
                     /locus_tag="AC06IO_000524"
     CDS             10011..11306
                     /locus_tag="AC06IO_000524"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIO9873739.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTL
                     KINGVKTVLRLIPSGMLRPNVTCYIANGVVLSPQALLSEIKELEGNGINVRERLRISL
                     ACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
                     KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGEN
                     ILFEGAQGVYLDIDHGTYPYVTSSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGG
                     GPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLD
                     VLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTVMSDL
                     PANARAYLKRIEEILGIPIDMLSTGPERDSTITLRGPFL"
     gene            11527..12096
                     /locus_tag="AC06IO_000525"
     CDS             11527..12096
                     /locus_tag="AC06IO_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HIO9873740.1"
                     /translation="MEYLTPRGLIPFITELKALNILQQDDNTNIALVSEILARWVVDK
                     DWLDEKFYYVNPEVGFNSWLIHEEKDHTLAVNIVAWQSGREITPHDHQTWGVVGSIIG
                     VEKNYFWKRMDDGSKPGYAEIIREKTAIIRPAGGVIGFLPNDIHSVMNESDDVAISLH
                     VYGKNLNYTGRHQYDPVNNLMQPFIVNYS"
     gene            complement(12296..12754)
                     /locus_tag="AC06IO_000526"
     CDS             complement(12296..12754)
                     /locus_tag="AC06IO_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIO9873741.1"
                     /translation="MTHDAFLDDLILSIVQTESIAEQSDLQNSLKKRGYEIPQATLSR
                     RLKKMKIAKVSGYYKIIDYNQPGLPLVLNVQISDFGLIILQTHPGNANSLAYYIDRKY
                     VSFSIKDSANSGILGTIAGDDTVLLIIKSKSDQQKVLDILMKEFPYLFAS"
     gene            12884..13618
                     /locus_tag="AC06IO_000527"
     CDS             12884..13618
                     /locus_tag="AC06IO_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9873742.1"
                     /translation="MKKICLLLLISSFILISSCSEQKNQNTLRFSTSAEYPPFEYIEN
                     GEIKGFDIDLAKLIAKELGKEAVFDNMQFSTVLPALNSGQDDVAIATITITEERKKNF
                     DFSIPYYFEGMAAVFDISQPVKEISQLTGKKIAVQLGSVMEIWLHQNFPNAEITALDN
                     NNQAIEALIAGHVDVVLMDGAQGGVYSKKYPGLSYSILAKANEGYGLVLRKDSPLRDE
                     INSALENLKAKGEIQKLESIWIKGSI"
     gene            13615..14262
                     /locus_tag="AC06IO_000528"
     CDS             13615..14262
                     /locus_tag="AC06IO_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIO9873743.1"
                     /translation="MTDVMQNLFFIGKGIVLTMQLLTGGMLIGVLLGTLLSICRHQGV
                     ACFVINRFISILRGTPLILQLSFIYFAVPVLLGMRPSILVAGIVAFGLNSSAYFAEIL
                     RAGIESLPKGQFEAALTLQIPTFYLWKDIILPQVARNIFPAMINEMIALLKETALIST
                     IGGMDLMRQAQTLAAQQFTYFMPLCITGFYYYSLVLLLEALGKKIEKRGHYAASH"
     gene            14246..14914
                     /locus_tag="AC06IO_000529"
     CDS             14246..14914
                     /locus_tag="AC06IO_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIO9873744.1"
                     /translation="MLQVINASKKIGNAQVLNEINLSIQANSIVGLVGPSGSGKTTLL
                     RCIQQLESIDSGSIQCEGKSGFMFQDFQLFPHMTVLNNLIYAPSLHDKTADHIANAWA
                     LLAMLGLSDKAHSYPQGLSGGQKQRVALARSLMMKPDLLLCDEPTSGLDMATTNDVIA
                     LLNTVKTLKITMLIASHDLFFLSNIAERLLVVKQGQLIADFKTQEVPDPILYLQQFYQ
                     GEIL"
     gene            14911..16128
                     /locus_tag="AC06IO_000530"
     CDS             14911..16128
                     /locus_tag="AC06IO_000530"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIO9873745.1"
                     /translation="MKKVIKKIALAYSGGLDTSIMIPWLKEHYEHAEIIAVICDLGQQ
                     EDLDAIKNKALKSGASKAYLVDVKNEFAIQYLWPLVKSGALYEDQYILGTISRPLIAQ
                     KLVEIALMEQVNAVAHGATGKGNDQVRFEYSLKALAPQLEIIAPWRAWDIKSRQEAIV
                     YAKAHGIEVPVTPKAPYSRDHNIWYISHEGGVLEDPSQEMPDDVLLMTSPVSQAPDEE
                     EMIILDFKKGVPVALNEQKLSPVDLLNTLNQKAGQHGIGVADIVENRLVGMKIRGIYE
                     APAAAVLYKAHKLLESLCLTRSTLHLKQSLQQTYANLVYEGRWFSQTKQALDAFIDVT
                     QQHVTGRVKLKLFKGNIIPAGMHSPYSLHHPALATFEEDNVYNQKDAEGFINLFSLSA
                     KIYSQVHQGGNYD"
     gene            16121..17356
                     /gene="argH"
                     /locus_tag="AC06IO_000531"
     CDS             16121..17356
                     /gene="argH"
                     /locus_tag="AC06IO_000531"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIO9873746.1"
                     /translation="MTNKTWGGRFKKSLDSGVNQFNASLSFDHVLFDQDINGSQVHVK
                     QLAKQKILTEAECQGIYSALEEIRTEIKQGQYSFNERDEEDIHMFIEQLLIQKIGDLG
                     KKLHTGRSRNDQVALDLRLYTRDKGCLINELLTRLIVCLDDLTSKHQQDLMPGYTHLQ
                     QAQPVALGAYFNAYQCMFSRDKSRLEDWFKRMNYSPLGAGALAGSTLPLDREWVAESL
                     GFAGIIPNTLDAVSDRDFVIELCSVAAMIMMHLSRLCEDLILWSTQEFNFVTLDDAFA
                     TGSSLMPNKKNPDVPELIRGKSGRVYGHLMAILTVMKGLPLAYNKDMQEDKEGLFDTI
                     NTIIVCLQMITPFLQSLTFNTPLMRTKAQSGYLDATAILESLVMKGMPFRDAHHQVGA
                     WIAEAIEKQCSLNELLKGG"
     gene            17359..18474
                     /gene="argF"
                     /locus_tag="AC06IO_000532"
     CDS             17359..18474
                     /gene="argF"
                     /locus_tag="AC06IO_000532"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIO9873747.1"
                     /translation="MSFSTSTNLTPIEMQDDDLEKIQSQNNPSAINEVKTNTFVINLQ
                     QAIFGLETGSEAIDFVSHKNTGFPFPAGEYKPRHFLTGTELAREEIQFILSQARSLKQ
                     NPGLYNQALANKSLVMIFEKPSFRTRLSFALAIQSMGGLVVESIANSRKQETPADMAR
                     VLNGYADFVMVRTHEDSFLQEMAEYSKIPIINGLSALHHPCQTLADLLSLEEQFGALE
                     GLTLTYIGDGNNILHSLMLLAPQLGVTVHYCCPKSNQPNEAIVKEAQSKAGKQMIYYF
                     DKPEEAVKQADAVYTDVWTSMGFDNPEAGKIFKGFQVNETLMSHAKPDSVFMHCMPME
                     RGKEVSATLPDSASSIIFRQSENRMHVQKALMLFLDQ"
     gene            complement(18564..20039)
                     /locus_tag="AC06IO_000533"
     CDS             complement(18564..20039)
                     /locus_tag="AC06IO_000533"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HIO9873748.1"
                     /translation="MNITRLLAFFTSFFLVCPIYASVDSYFSHIKKDSSALYAFFKTM
                     PKGGELHYHLAGGAYPETMLALAANGNYCLDKLSFAVSKDSSHCEGVNVKEVFNNPEL
                     YSNIIKDWSMKDFIPGKESGHDHFFNGFVKYMPIVFDYRPQLLADIVQRAAQQKEQYM
                     EIMIIPDNAQSLTYGDLIKDIPSYDQKRRLLLANKDFQSNIKNTVLESDRILTKASQE
                     LGCKDNPSSDSCHVKIKFIYYVLREQPVDNVFAQALNAFEAVSKSKGNLIGVNLVQPE
                     DGIISLRDYHKHMSIFEYLHRIYPNVPITLHAGELSPQAVTPENLSNHIRDALLIGHA
                     QRIGHGVDIGYENNAEDTLKYMANHHIPVEINLISNLKILNISGRNHPLNYYLTHNVP
                     VVLSTDDEGVLRTDLTQQYVEAAKEHGLNYQQLKQINRNTLTYSFLPGKSIWANADKA
                     ELIPDCHDLGSKSCQKFIEHNEKAKLQWSLEQQLIAFEKNY"
     gene            20213..21328
                     /locus_tag="AC06IO_000534"
     CDS             20213..21328
                     /locus_tag="AC06IO_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9873749.1"
                     /translation="MPYFYTRSAPLFFVFVIALIFINFSYAHEFQTDSKNKHKVDVHI
                     GIYAPFSSHSAFIGRNILAAMEMGSERLQSSDIHYSFYTLDQLPKNKNAGLVLQKFID
                     THHINVLVTGGKETGLIAAPIAKKNNLIHFSLTNNTEVADGRNNFIVWSPAYDQAKVL
                     VNELKRQQVHHLGIITANQPSSTALTHSVIKQLQADSHIDVAASEYFEPGNKDFSRLT
                     NRIKRKNADMYLVMASPEDIKLIQEQMEADKQHVPITSIVERATPEVVKVFEGQWYVD
                     SHEMQPDFVNEFKQNYLNYPVTEAGYAFDLFQLLNKSIVMAIRTQGSPTSQEVVHQLH
                     DIANGTGVMGTMVLDSKGSLFTKSEVKTVKNGEIHTV"
     gene            complement(21401..22738)
                     /locus_tag="AC06IO_000535"
     CDS             complement(21401..22738)
                     /locus_tag="AC06IO_000535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIO9873750.1"
                     /translation="MVIKKLFSSTLALVYALTLMLPLTAFSAEETNSNYSNTKRIPLE
                     DVQRFSNAIGEIKKYYVKPVDDKELFDNAIRGMLTGLDPHSSYLNEEEFKELQTSTSG
                     EFGGLGIEVTMEEGVVKVITPLVDTPAFKAGIKSGDYIIKLGKESVQGLSLKDAVNLM
                     RGKPGTTIELTILRKGVNKPLTFDLIREVIQIKSVKSKMLSEGYGYIRLTQFQALTGK
                     DMIKAIEQLKQQAGGKLKGLVLDLRNNPGGLLDSAIQVSDAFLGNDKAGKQEMIVYTE
                     GRLPGSKFTALANPGDVLDNAPIVVLINNGSASASEIVAGALKDNKRAIILGTKSFGK
                     GSVQTVLPLDGKTGIKLTTALYYTPSGVSIQAKGIIPDIIVEEVDVPKNAVKKDTLAG
                     FTEADLNGHLINKDNSENTESKNNQIQTGDLIHEDYQLYAALTVLEGMALANR"
     gene            complement(22819..23961)
                     /locus_tag="AC06IO_000536"
     CDS             complement(22819..23961)
                     /locus_tag="AC06IO_000536"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIO9873751.1"
                     /translation="MRMPSLSNSLSGASLGLALMVILFFCNGLEVKAAPNSITQAQTK
                     LKQLDAKINKLKQVLANAQDKRTVLNQELGSTEKQIGEGIRKLRIIQANMSAKEKSIA
                     ELQGKINHLNKRLQAQQEMLANHVRARYQMGEYQPLKWLINQDEPFKINRILTYYQYI
                     IRSRQQLIDEIARTRQNLNDSKTKLGNELAENKQLQFKLSQNQKQLEQNKRYHSTLIQ
                     SIDNDIQNNQQSLKDFQKDRDNLSHLMKSLSQQSVIQSGKPFTGMRKKLPLPVQSPHR
                     SLRRMNQGVTFFADEGSIVTAVYPGKVVFSDWLKGYGLLLIIDHGQGFMTLYAHNQSL
                     FKRKGQIVHQNEQIASVGHTGGIKQNGLYFEIRQRGKAVNPLDWLS"
     gene            complement(23948..25492)
                     /gene="gpmI"
                     /locus_tag="AC06IO_000537"
     CDS             complement(23948..25492)
                     /gene="gpmI"
                     /locus_tag="AC06IO_000537"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIO9873752.1"
                     /translation="MSHNAPLVLMILDGWGYNENDRYNAIAKANTPQWDEWWQTCPHI
                     LLKASGLPVGLPDEQMGNSEVGHMHIGAGRVIQQDFTRINEAINNGKFAKNAVFHEVI
                     DQLKKTEKSLHIMGLLSPGGVHSHEQHLFALLALCNQKKFRSVHLHLFLDGRDTPPQS
                     ALDSLKCLNEELAKHPVATINSICGRYYAMDRDKRWQRVEPVYNLLTQGKSERQFPDA
                     ETAIHFYYKNKISDEFVPPTLIGEEHSIQDGDAVLFFNFRADRARQLTSIFLDPSFKG
                     FERKTLPKLSYFVSMTQYDKNLFTTTAFPPVPLNNTLGEVLSSHGLSQLRIAETEKYA
                     HVTFFFNGGCESVFTNEERIMVPSPQVATYDLQPEMSAHELTKTLIAAINSQDYHVII
                     CNYANADMVGHTGNFEATVQAIECLDQCMQQVWQALKNNGGKLLITADHGNAEEMFSE
                     ATNQAHTAHTSEPVPFLYVGGGWHFTHSEGSLIDIAPSLLALLGITPPPEMTGRILLE
                     KNHAHA"
     gene            complement(25595..25891)
                     /locus_tag="AC06IO_000538"
     CDS             complement(25595..25891)
                     /locus_tag="AC06IO_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873753.1"
                     /translation="MSRDTKNLILKAIILFVSLTALVNHAMAMNLLGIGPNPKNLPKD
                     NDVRATFNHCKSCDKSTTPPLIITTPVPIIYYPGCRPIQSCDAYGHCEQKEDCY"
     gene            complement(25968..27239)
                     /locus_tag="AC06IO_000539"
     CDS             complement(25968..27239)
                     /locus_tag="AC06IO_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HIO9873754.1"
                     /translation="MKKSIRRLVKPRLFAMSISLLAFSSITYGESNGSFALSEHWSMG
                     QQIKLRFDINEVPQSGVVLHLKNGLALTAGDIISLGDLYGIVGKPISHGLTKLEKQTR
                     FKEVFNSFARNAIAVSEVEDLNSVIRAEMREVESGIAKGETAEAIYKRIGNEVGRQIN
                     CITGGGCNSLTWWLNPGRYLKLAMENYDHFTPNNLIAYKSGHHVALQQALKAKRTGKR
                     SDLEIAYAMDAFACHYLSDHFAAGHLRTPREELSAIITPTVLGALLSNYMHNEENKYG
                     IHVTNNLGEQWMVYGDYSYFNPFNQTNQQMLLKALQASADEIFEAYYSGVLPEKNQIE
                     DMIPHITKLNDENNMDIAPMFYWDKENKQLLRRTNLTNPYDRHWTNSWWGWSTLLLLK
                     TQYGITSTVQLSLSKYLSQYVPEELQDNPLV"
     gene            27589..28305
                     /locus_tag="AC06IO_000540"
     CDS             27589..28305
                     /locus_tag="AC06IO_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIO9873755.1"
                     /translation="MNHKLPQHIAVVMDGNGRWAESRGLPRVEGHKAGLDSVKKIINC
                     CLEKKISCLSLFAFSSENWSRPVTEVNFLMELFLEALRKEIDDLNQHGIRLKFTGDRE
                     PLSQVLQKQMSDAEALTKNNQQLILNVVVNYGGKWDIVTAARKLIRTVLDGKLAYDEI
                     NEAVFAQFLDTNGMPEPDLFIRTSGELRISNFFLWQLAYTELYFTDVHWPDFNEHEFE
                     LALISFAKRARRFGQISQSE"
     gene            28316..29113
                     /locus_tag="AC06IO_000541"
     CDS             28316..29113
                     /locus_tag="AC06IO_000541"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIO9873756.1"
                     /translation="MFMQRLITTLILAPLVLLAIFYAPSWILWGVVFVILLLAGIESL
                     KLIPVDNLSMQACFLIFLLMCFWACGYLYPYWLSVGLFVWLFNGLAILTYPRSQNYWG
                     YPMIVAIACLILLPLFTQSLIQISFLQQGKELLVYLLFLIWASDIGAYLTGKQWGKHK
                     LIPKVSPGKSWEGVTGGVILAMIVATLGYYYFIPYSIYIWFGLALSTVVISIFGDLFI
                     SILKRRCQLKDTGNIIPGHGGILDRLDSLIAALPWFYFGLTYIPLGI"
     gene            29146..30498
                     /gene="rseP"
                     /locus_tag="AC06IO_000542"
     CDS             29146..30498
                     /gene="rseP"
                     /locus_tag="AC06IO_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIO9873757.1"
                     /translation="MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKI
                     LARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGP
                     LFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLVPKQEILALNGVKINSWR
                     DFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIIPFIP
                     TIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKR
                     QGKLLNITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTVQ
                     LTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALN
                     LLPIPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
                     "
     gene            30669..32981
                     /gene="bamA"
                     /locus_tag="AC06IO_000543"
     CDS             30669..32981
                     /gene="bamA"
                     /locus_tag="AC06IO_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIO9873758.1"
                     /translation="MKKVSNKLILGVCCSTLLAWSSQSFSSDTFIVKGIRVNGLQRVS
                     TGTVLNYMPVQVGEEISSSSTAQIIRALYETGFFQSVSLERQGNVLVVNVVERATIGS
                     ITVVGNKEIPSDKMKAFLKEMGLVKGRVFQRSSLERLEKELKQAYTARGKYNSRIETK
                     VTPLTENRVAISITVSEGRVSRIKEIKIIGNHDFKANELLPELTLSTSNLFTYFTKKD
                     QYSKAGMDASLEALRSFYLDRGYLKFNVVSSQVLLSPDKKDVYINIHIEEGPQYHFSG
                     YDVVGKTILPKEKIDSLIQVKKGDIFSRKKVTESISAIGLALGDVGYGFPAINAEPRI
                     DENNKTVFITFVVQPGRHVYVRRINFHGNTKTGDYVLRNVIRQDEGGLLSLHNIKESE
                     RQLRMLGYLKNIDVKTTPVPGTNNQVDLDVNVEEAPSAEASASMGYGTNGYQFNASVN
                     QRNFMGSGRSMGAAFNASQWGQDYSFNYYNPFYTDTGVGRGGSLYYSRIDPKNLNVST
                     YSSNRYGGDISYNFPLGEKSSFQLGYGYQDINIKSVGYVLPIINFVALNGNHFQEIRL
                     TSGWSRNSYDQMPYPNQGFNQQAIAMVALPATSQSLSYYKSSYQAHLYYPLTRGWIFS
                     VLGNVGYGNTFDNFGLPFFENYYAGGPVQPGQVRGYDSYSLGPQDNFGNAMGANFLVN
                     GSVGLILPYPLSRDNVRTTIFADAGNVFASGTPPALRGTPAGPMRYSAGVSLEWRSPF
                     GPLSFSLAKALNPQPLDQTQLFQFALSSGF"
     gene            33095..33595
                     /locus_tag="AC06IO_000544"
     CDS             33095..33595
                     /locus_tag="AC06IO_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HIO9873759.1"
                     /translation="MKRLGAVLVALFLGLFGTTVFADWAKIGVVDLQKIMQTSNQMKE
                     IQQKLEKEFKPRRDKLVAMEASLKSDMEKFKRDSAIMSASQKKELEKKIVASQQQFER
                     DGQQYQQELSTAHNEAMEGLYNKVRTAITKIAKDEKYDIIVQKDAAPFSSESLDVTDK
                     VIKAIN"
     gene            33609..34628
                     /gene="lpxD"
                     /locus_tag="AC06IO_000545"
     CDS             33609..34628
                     /gene="lpxD"
                     /locus_tag="AC06IO_000545"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIO9873760.1"
                     /translation="MLTLEELANYLNGVWHGNANDAIFSLSSLARASSKDLAYFDNPL
                     LQSVLNSTRAGAVLLKPEYKDWCPVNCIVVPNPSESMFKAAKILPHLGTNVCGIHPTA
                     QIHKSAQIGQHVSVGSNSVIGENVQLDDYVSIGSGTTIESSVLIGRGSQLGSGAIIHS
                     GTVLGQSVIIDSGCIVGAAPFNCYKEHGVWQQAPNFGGVVIGQRTQIGANTVIHRGSI
                     GDTYLGDGVCIDSLVLIAHDVYIGNNTAIAGSAAIGALVQIGSDCIIGGASCLAANIR
                     LTNDVVITGMSTVTKSIARPGIYSSGTTVHDHRKWRRNTARFRHLDDYITKLITIEKK
                     LNNNN"
     gene            34746..35198
                     /gene="fabZ"
                     /locus_tag="AC06IO_000546"
     CDS             34746..35198
                     /gene="fabZ"
                     /locus_tag="AC06IO_000546"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIO9873761.1"
                     /translation="MNESIDINQIFTLLPHRYPFILVDRVIDYKVMEYLIAIKNVTIN
                     ENFFTGHFPGNPIMPGVLMLEALAQASGILANLSRQPKEGYEFLHYFAGIDNARFKQV
                     VIPGDQLRLEVRLVGQKRDFWRMHGEAYIGDKLACSADLLSATKEIKK"
     gene            35195..35965
                     /gene="lpxA"
                     /locus_tag="AC06IO_000547"
     CDS             35195..35965
                     /gene="lpxA"
                     /locus_tag="AC06IO_000547"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIO9873762.1"
                     /translation="MIDERAMIHPSAKLASGVSIGPGTVIGADVEIGENTWIGPHVVI
                     EGPTVIGKNNKIFQFASVGDEPQDITYKGEPTRLEIGDNNVIREYCMISRGTVKGGGV
                     TRIGNSNYLMAYSHIGHDCMVGNHIIMVNYAALSGHVTINDYAIIGPYAAVHQFCQVG
                     AYAFIARATYVTKDVLPYVMIAGHTTSACGINTVGLRRRGFSSAAIDCLRRAYKIIFR
                     KGLTVQQAVSELELIQNECPEIIPMIDALNQSTRGIVR"
     gene            35969..36373
                     /gene="crcB"
                     /locus_tag="AC06IO_000548"
     CDS             35969..36373
                     /gene="crcB"
                     /locus_tag="AC06IO_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIO9873763.1"
                     /translation="MVVAPYLAVAIGGSLGAMSRYLVTIMAQNAWGIKFPYGTLLVNT
                     LGSFLAGFFLIVLVGRFSAEESFRLFLFTGFLGAFTTFSSFAAESLFMFEQGYWFKLI
                     TNILVNNVGSLSMVFIGTLVAKYVLLGHQGSN"
     gene            36395..37675
                     /gene="serS"
                     /locus_tag="AC06IO_000549"
     CDS             36395..37675
                     /gene="serS"
                     /locus_tag="AC06IO_000549"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIO9873764.1"
                     /translation="MLDNQLLRENPQYVATQLLKRGFQFDAVTFSQLEEKRKALQVST
                     QSLQNERNLRSKAIGEAKSRGENIEPMREEVNKLGAKLEQQKTDLEEVLKQIEAITLS
                     LPNIPHESVPVGKDELDNQEIRKWGDVPAFSFPVKSHDELGEALGQMDFALAAKITGS
                     RFVVMKGHLARLHRALIQFMLDIHIQQHGYQEIYVPYIVNADSLLGTGQLPKFEADLF
                     KLTGDNGYYLTSTSEIPVTNTVREMILSAEQLPIRYVCHSPCFRSEAGSYGKDTKGMI
                     RQHQFEKVELVWITKPEDSYNALEQLTQHAEVILQRLNLPYRVVALCTGDIGAGSAKT
                     YDLEVWLPSQNTYREISSCSNMEAFQARRMKARFRNPETNEIQLVHTLNGSGLAVGRT
                     LVAIMENYQDEHGNIHIPDALKPYLGGIDIISVK"
     gene            37929..38972
                     /locus_tag="AC06IO_000550"
     CDS             37929..38972
                     /locus_tag="AC06IO_000550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9873765.1"
                     /translation="MNAVIIREITSEDKEMFLCAMQSSQALHHPWVKAPIIPEEFDEY
                     FSRYQKPNQKSYLLLSDNNLAGVFNISEIVRGYFQSAYLGFYAVAAYAGKGYMSTGLK
                     LILTKVFKDMGLHRLEANIQPENTRSIWLVKKNGFRYEGFSPRYLRVNDVWQGHEHWA
                     MTYEDFIKDNNEVLEKDHIDIVAYNTEWPLLAKAEMVKLRASFPANSVIDIQHVGSTA
                     IPGMAAKPIIDIQIAVRSLEEMKIIAVPILQKLAYEYWEDNPDPERMFFVKGMPPYGN
                     GRTHHVHIVEASSRHWKGKTFFRDYLLSHPEAAEKYQQLKIKLAQERKYDREQYTDAK
                     SEFINQILKLAQN"
     gene            39142..39405
                     /locus_tag="AC06IO_000551"
     CDS             39142..39405
                     /locus_tag="AC06IO_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873766.1"
                     /translation="MADQPPKSKWHEIKRLSEGDNKVDNNQAPVATADRIKKLYLDTQ
                     VGSPIDTVEEADAQRNNLGPKDTKIENSSHENDEPNPDIPTPG"
     gene            39745..40677
                     /gene="legD2"
                     /locus_tag="AC06IO_000552"
     CDS             39745..40677
                     /gene="legD2"
                     /locus_tag="AC06IO_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegD2"
                     /protein_id="HIO9873767.1"
                     /translation="MDNLLNEKKLTIFTSNEIRDNLNQILKDYEQNGIIGLNGYLNQA
                     NRINLLNELEVMQKDAEQDIARYWNNKIIHFYSKDPLKPESVDREYVTEPYFQASSNK
                     AHVFYEVIDNMLVVNRIGHGMHLINRYPMMQRAVYDSPVLLDLLKGIGFKKPICHLSV
                     YIPKYPNGIGSEVRPHQESTFAFTEPQSVVVLWVALEDALIENACMYGVLGSNHWPLK
                     WVSKVDRETKTRHFEQVHQLHIPDFITEREFYTALEVKAGDALLFHGNFVHCSPMNTS
                     KNSRKALSFQFIETHEVDYPETNWLYPPNKVYLY"
     gene            40713..41525
                     /locus_tag="AC06IO_000553"
     CDS             40713..41525
                     /locus_tag="AC06IO_000553"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9873768.1"
                     /translation="MSMYDKRQFFKTYFSSASEKSILVKLLDKRFHPPSHTANILDLG
                     CHDGALMKNIVNAYAKRLPEKLVITGVDPSLQAIKEYAKADFPIPVQTNTFAGTAEDY
                     FDVCTDHFDWIFASQCLYWSPDLPKIIKQIHETGDSALIVIRGCHGIYEIQSQFRHYI
                     GNHLEKFYTDKEVEMALRTQKILFQKESLSTSIRLPDKDSIELKWLILFFLQMEEGSL
                     GDKIFCEVRDWITSRTSGSIHHEVHFFWLGNAIFTGDYNMNFPVKSKKNFMG"
     gene            complement(41515..42345)
                     /locus_tag="AC06IO_000554"
     CDS             complement(41515..42345)
                     /locus_tag="AC06IO_000554"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HIO9873769.1"
                     /translation="MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYY
                     EEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDHRKPYDDTASLTIQVQQSFASSL
                     EHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDEKVKHLGISNVSYEQLVEL
                     ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHEIIYQGFSLLTANQYELRHPKLENLS
                     KKYEKSIEQIIFRFSQQIGMLPLTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
                     "
     gene            complement(42784..43632)
                     /locus_tag="AC06IO_000555"
     CDS             complement(42784..43632)
                     /locus_tag="AC06IO_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0518 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873770.1"
                     /translation="MTDLRTEIESLIHNGLLPDREYVSAKRFINHYANTNEFWAGLAL
                     VGITPIINGLYACYYASRAVWATLRTVGNLLILKPGLALDAIKDVGCHLTLSTCLLLM
                     APIHALTHAVEILTRVISSWFVGQEPRHDLLETGLMAKWANRVSGYQMVNELPSSNYF
                     NKARFFSSYNNAWDCLEQMASPMTSLAKSGFHSLVEALYGVESAIECIVNLAICKPLH
                     AKENFHNLQVHFGLAVSLAVMTPINALVEGIAFMTRLGTTWVSACLKPEVTETEHENT
                     TAYQFN"
     gene            complement(43696..43920)
                     /locus_tag="AC06IO_000556"
     CDS             complement(43696..43920)
                     /locus_tag="AC06IO_000556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873771.1"
                     /translation="MNAENNVLNTLNSLSKEINKLYGYVTIAGDNFGEPAINSGPCGP
                     FANAFYWVFWSVFKRHWKTDANIKYNMGQY"
     gene            complement(43904..44662)
                     /locus_tag="AC06IO_000557"
     CDS             complement(43904..44662)
                     /locus_tag="AC06IO_000557"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIO9873772.1"
                     /translation="MTKLRNLDTYLSLCTEVYDLSKPNPPEDAYAFYRSYVMNVKDPM
                     LEPMCGTGRFLLPLLEEGFEIHGFDASEYMLEALNIKAKAKNLKPTVWRGFVENLTRP
                     EKYNLIFIPSGSFCLLIDPATVRLALQTFYNHLSDEGVLLFEGETLKAVPQLDVWRGS
                     VWHKPNGQMIMLSQLATMKDQVCHSIGKYELVHNNSIIHTEVEELRVRIYDPQELTGI
                     LKSCGFKNVRLIKAFDSAATPDAQDETIVYECRK"
     gene            complement(44628..45236)
                     /locus_tag="AC06IO_000558"
     CDS             complement(44628..45236)
                     /locus_tag="AC06IO_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIO9873773.1"
                     /translation="MTPHIIGISGITGAGKSTLAKALSLDLKATLISWDDFDDISLEP
                     EDYIEWYHKGCNYSEFQRENLAKVLAELKAKREITHPVLRTLLNPAEYIIFDAPLGKL
                     HVQTGEYIDTCVHIEVPLDISLCRRILRDFKDKLQTKENLLEEISFYLNHSRPLFFDD
                     ELKQNATIVIDGLLSTEVQIQIIKNFLIGEQDDKIKKPGYLS"
     gene            complement(45233..45700)
                     /locus_tag="AC06IO_000559"
     CDS             complement(45233..45700)
                     /locus_tag="AC06IO_000559"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9873774.1"
                     /translation="MITIDLLKNHPQSIARCATIWHEVLGKIWVPEISVEQVIQRFSE
                     HLNADKLPITFIALDTGKPVGMCSLRENDGIRPEINPWLGSLVVDPAYQRQGIGNRLI
                     EATKNKARDLGFNKLHLFAFDPTIPNYYSRLGWAIIGMDKFKGLPVTVMEILL"
     gene            complement(45852..46415)
                     /locus_tag="AC06IO_000560"
     CDS             complement(45852..46415)
                     /locus_tag="AC06IO_000560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9873775.1"
                     /translation="MNINFEKANASHIELIFSWLKEPFVQEFWDNTQAHKDDILNFIN
                     GRTEPSSYADGKYVYWIASGNGHPFAMLMTIQETSEDEIDEIKLINLSKTGHSYGIDY
                     MIGDKNYFGIGYGARTLIEFIDFFIKEVDPKADTFLIDPASDNPRAKHVYIKAGFEYI
                     ADFLMSGDVSGAGKLHHLLIKRIGLIK"
BASE COUNT        13894 a         8153 c         9640 g        14792 t
ORIGIN      
        1 cctgcaggga aaattaatta ttttattgca ataaatggtt gtttcgcttt agtcattgcc
       61 aattattagt cacaattgca cgctgtcttt atgactaagc ataagtttcc atagtagatt
      121 tagttctact tatgatagca tgtccttgat ttaaaaatga atgaggtatg attgcgaatg
      181 aataaagaaa atcattctgg gatttatttg ttaccaaatt tattaacgac tgtcagttta
      241 tttgcagcat tttattcaat agtggcttcc acaaagggac aatatgaggc tgctattgtc
      301 gctatgttta taggaatgat cgctgacagt cttgatggac ggatcgcccg gttaaccaat
      361 acacaaaccg agtttggtgc tcaatacgat agtttgtctg atatggttac ttttggagtg
      421 gcgccatcct tgctgttata taatttaact ctgcatcaat taggaaaatt tggttggttg
      481 gtcgcattta tttatacagc ggctgttgcg ttgcgtctcg ctcggtttaa tactcagatt
      541 ggaaaatcag ataaacgtta tttccagggc cttccatgtc caccggctgc agcagtaatg
      601 gcttcttttg cttggatctg ttttcaaaat gaatgggcaa atttgattgt atctttattt
      661 acagctgttc tggctttgat tgtttccgta ttgatggtga gtaatttacg ctattacagt
      721 tttaaggaag tagattataa gggcaaggtt ccatttttat atgttttagt gatgattctt
      781 ttgtttgttg caattgctgc aaatcctgca atagtgcttt ttgcaagttc aggtatttat
      841 gctttatcag gccctatgtt gacggtgatt tcattacata aaatgaggaa acaaagaaga
      901 aatatgagca aatgacatcg attgtgtatt tcttgatgaa ttattatgta aatctgttac
      961 ccagacctgt gaacagtaga tttcttcaga ttagtatcga ttaaggtgta ttgagatctt
     1021 aggtcagttt attgaatcac aggttgatca taataaagat gttacaagaa cgcgaggaac
     1081 attgatggag aacaggaacc tctgtgtgtt gcaaaacgat atggagcaga ggtaaacagc
     1141 gttggttagt attctttatt cgctttcccc aaatcgatta ttgattaatt cgactaaagc
     1201 ttcattcatg cgttcttcat ctggaccgtc aatttccagc gtcaactcac tacctttgtt
     1261 agcagcaagc atcatcacac ccataatgct tttgccatta acggtctgag tatctttggt
     1321 tacatcaata tggctttgaa attttgctgc tgttgagaca aatttcgctg aggcacgagc
     1381 atgtaaaccg agtctgttga ttatctttat tttggtcttg atcatagcac atacaatgta
     1441 tcatgactct ggcatcaggt acagtgatta aataagtgat tttagtaatc aaacctcttt
     1501 ctcagtgcta tgacctcatc taaaaaacgg ttatttgttt tatgtcagtt tacataagca
     1561 ctgagaaggt attgatatta aattaaagta aaagggtttg tatttaaaat aacatttaat
     1621 ccctgtctct atgggctaaa agtttttctt tatgtttaat cagttgtcga tttaatttgt
     1681 ctaccaaagt atcaatagca gcatacatgt cttctgattc tgaactggca tgcaactcgc
     1741 ctttggtaac caatacggtc gcttctgcta tttgtcttac tttttcgaca tcaaatacta
     1801 cattaattgc tctgatgtga tcaaaatgac gctcaagctt atcaaatttt tcttcagtga
     1861 atgctcttag tgcaggagtg acatccatac catgtccagt aatgttgatt tgcataaaac
     1921 ctcctttcgt tgaccgatta attaactact aataaccttt ctttcattgg atgggggaat
     1981 ccccattgct tcgcgatatt tggcaacagt acgtcttgct actttaatgc cttgctcacc
     2041 tattagttgg gcaattttac tatcactcaa aggttttttt ctattctctg ctgaaattaa
     2101 tttttttatt accgctcgga ttgcggtgga agagcattct cctccgctat cagtattgac
     2161 atggctggag aagaagtact ttaactcaaa aacacctcta ggagtgttaa taaatttttg
     2221 agttgtaact cttgataccg ttgattcatg catatccaag gcagaagcta catcattcaa
     2281 tattaatggt ttcatcgctt cttcaccaaa ttccagaaat ccatgttggt attccatgat
     2341 acaggttgct acttttagaa gtgtttcctg acggctttga atgcttttta aaaaccaacg
     2401 tgcttcttgt aaattatgct ttaagaattg attatccaga ctattgtttg cacgttggat
     2461 taaagcggca tattgactat tgatactcag cctgggaaga atatcttgat tcaaaaaaac
     2521 tttccattga ttatctatct ttttaacagt taagtcggga ataacatatt caatattatc
     2581 ttgatggatc aaacttcctg gttttggatt taatttatga atgacttgta atatttcatg
     2641 aattgttttt tcacttattt gatgattttt catgagttgt ctgtagttat gttgtcctag
     2701 caactcaatg tcttcaagaa tgactttctt cgcagtagcc aaaagcttgg gttccagtgg
     2761 aagttgttcc aattgcaaaa cgagagtttc tgctaaatta acagatgcgc atcctacggg
     2821 gtcaaataat tgaagcctgt gtctcactgc ttctatttct tctatatcca gagggaaatc
     2881 atcgctattt aagctggcgt gaatttcagc aacgggtata gttaaaaagc cgtcatcgtt
     2941 tatcgcatca ataattgttg tagcaatgac tcggtcaata tcgctcatag gcgtcaggtc
     3001 aagttgccag ttaagatgat cttgtaaatt ggtggtagtg cagtgtaaat tatcaaaatt
     3061 gtaatcgttt tcttcaaaat tattgcgctt gtttaagtta ttatacagtt gagaccattg
     3121 aaaatctgaa tattcatcat ggttctgatt ggtatttatt tgggagtctt ctttttcttc
     3181 aacaggagtg gcttcaagca aaggatttga ttcaacaacg agttgaattt cttgctgtaa
     3241 atcaaaggtg gataattgca gcagtcttat ggcttgttgc agttgtgggg ttaacgtgag
     3301 atgttgacca ataccaatct gcaatgtcgg tttcattcaa atcctctttt ttttcactgt
     3361 ctttaactat cagtttaaac gatccacaag gattatccag tgcttacggg cgttaatttg
     3421 aatttaaaag taatggaatt acctatattt ttaataaaat gataggcttg ttttgaaata
     3481 taacaaattg gtttttaaag tggataatga taggacgtaa gccccaaaga caggggccta
     3541 tcaatttatt ttactttttt ctctttgaaa agcacgtgct ttcgtacttt agggtcgtac
     3601 atcattaatt ccatcttttc aggatggtta cgtggatttt tggtagtggt tttatagtat
     3661 ccagttcctg ctgtggattc cattttcact ttaattgtaa cagctgccat ggcttatccc
     3721 cttgttaaat cttctcgcct ttagctctga ttttatccag aactgccttt atgcctaact
     3781 tgtcaataat tctcataccc ttggtactta accgcaaggt gacaaatcga ttttcttctt
     3841 caacccagaa tttatgattc tgaatgttag gcaggaagcg tcttttggtt ttgttattag
     3901 cgtgcgacac tttatgacca accatgggtc gcttgccagt tacctgacat actctagaca
     3961 ttgtgttact ccaagataaa aaaaatttta acagccctta ccaatgcact acagtggtgt
     4021 ttattttggc gggctttcgt tgtaaaaaat aaccagatat gaaatacaag agcggtttta
     4081 taacaaaaaa acccaaatat tgcaatattt ttactttgtt ttcattaaaa actgattttt
     4141 cagggctgtt gcccaaacgg gtatctggta aaagtagaat atttaatcaa aatcagtata
     4201 atcctgaaat tttatattga gttattgtga tgcaacgtaa gattaaaagt tacgtgttgc
     4261 gcgctgggcg aatcagtaac agacagcaac aaggattaga tctctggctt gaagactatg
     4321 aattaaaatt tgatagtccc actccctgga attttgcaaa agaatttggc cgtcatgatg
     4381 cggatactat tgtagagatt ggttttggta tggggacctc gttgtttgcc atggcaatga
     4441 ataaccctca atgtaattat ctgggcatag aagtacataa agcaggagtt ggtagtctgg
     4501 tagcggactt gcatgagtac cagatatcta atgtaagggt agtagtgcat gatgcagtag
     4561 aagtattaca aactaaaata ccagaaaact cgcttgccgg ggttcaaatt ttctttcccg
     4621 acccgtggca taaaaagcgt catcataagc gacgactgat tcaatccgaa tttattcaaa
     4681 tgttagtaaa aaaaatcagg ccatctggat ttattcactg tgctactgat tgggaagatt
     4741 atgcagagca cattttaaat gttttatctt cagaatccgc cttgtttaac caacaaaaag
     4801 agggcggata ctcaccacgc ccggattcaa ggccgttaac aaaatttgag ctgcgtggtg
     4861 agcgtctagg ccatggggta tgggatctgg tttttataaa aaaataagga agttatctat
     4921 gataaagcat gcgattcttg ttagcggatt gattgtttcc tttttagctt cagcagctga
     4981 aatgtcattt ttggcccaat tgacggaggt acctggtgcc tctgggtacg aaaaaaatgt
     5041 tcgtcatttg gtgcaaaaac agtggcaacc ctatatggcg gagctaaaga tcgatggtat
     5101 gggtaatttg atcggccagc gtagagataa tcagaaagga ccgaaattac tcttcatggc
     5161 acaccttgat gaaacggggt ttatggtgga atccataaca cctgatggtt ttttgaaagt
     5221 tatcccttta ggcgggatcg caaattcagt catctatgct caacgctgga cagtttcaac
     5281 acctaaagga tctgtattag cttattctgg tatggattct ccgcatttat tgggtgataa
     5341 gaaaatgtta gggtcccctg atatcaatgc cctgttttta gatatcggag cagagaataa
     5401 agaacaagct cagaaagaat ttgcaatcca acctggcctt gaagtgacac cagtaagtga
     5461 atttagccaa ttaagcaaga acagattttt aggcaaagcc ttggatgatc gcctaggatt
     5521 agctgtcatt agtgacgttc tgagagcagt gaaacaacaa cccaatcaat tatatcttgc
     5581 tgccacagtg caagaggaag ttggtatgcg gggtgccagt acagtataca aagcgactca
     5641 tccagatatt gcaattaatg ttgaagtggg aatcgcagat gattatccaa tgctgctggc
     5701 tgatagaaaa ggtcgaattg gtttggggaa gggaccatcc ttatttgttt atgacaaatc
     5761 aatgattccc aatcaggagt taattaactg gataatggaa cttgctaata aaaataatat
     5821 cccgctacaa cttgaggtgg agccaggata tggtgaagat ggctctaagg ttcaggcatc
     5881 aggcatgggt gttcctgtcg tcaacattgg tattcctatt cgatatgcac accaacatgc
     5941 cgggatattt gataaaagag attacgataa tacagttaaa ttgttgagct tgattgctga
     6001 gaatttgaat caggaagtgg ttgacaagat taaaaagagt taacaatatt ttgatctccc
     6061 attgtccatt gtgtaatatt ggtgtcctat aaatagttta taaataaagg aagtattatt
     6121 atggattttg taagtgaaat gaataagatt ctacaaatgg aattagagca atttaaagaa
     6181 tttattcaaa agaagctgga agaagataaa aattatttag aaaagttatt ttggttgccg
     6241 aatggttctc aaatgactgt tttaaattat ttgatcgaac aatactctga gcctaagctt
     6301 ggtatagacg atgcaaatag agatttgctt gccagaatag attttgtcct tcacgaggct
     6361 aaggatgtga atgttggtga gcccttgcac caggcaattg ttgctggaaa aatttccttg
     6421 gcactccatc tgctgggagt ggatgaaaat aatttcacat ccggggagag tgaaaaaacg
     6481 gatgttttga gtattttgtc taaagtaagg aaaaaaatcg aagagtcttt taatcacgta
     6541 aaaagatgtt tttttgatgt ggataaacgg gatggttatg ggcgcacttt gttgtctttg
     6601 gcattagatg ccaaacgcca ggaactgtta attgccattt tagccaggaa tcccattgta
     6661 cacgcgacaa cgttaagatc ctcagcttat gttcctttcc agcctattca tcaggctgtt
     6721 gttttagatt atgcagaggg cattacccta ttagccagca tgggggcgca attgacaaat
     6781 cccctgggca gcatgagaga cactccggtc attttggccg ctcgtttggg caaaataaat
     6841 gcactggcgg ctttactgga gttaccaact caaagtttgt ctcttgaatc cgaaaacaat
     6901 catttattcg aagacaaaca aactggccat acagcagttg aagagttatg tgaacgcatg
     6961 gccaatgaga atgataaagc agacgctctt cgtgggattg ccatgctaat atgccgtggc
     7021 gctgaaccac cccgtaatga aaaaatgcga aatttgttaa gcagcaaccg ggttgctttc
     7081 ttaaaagcgg tcagtaccta tttgggagat aagcctcaat tggtcgatgc ttttgtggag
     7141 cgttgtcatc taagagaaag cgctttgcat aatatagttt atgccgatca ttcatggggg
     7201 agttctattc gtcatttact cggtgttccc agtgaggcag cccttattgt tgaagaatta
     7261 gtaacgagaa aatacagtga tccttccttt gccagcgaaa atgtttcgtc attatcaact
     7321 actgccacgg aaaacctacg ttcagagagg aacccactga aactctatgc cgaatttgtc
     7381 aggcgataca cgcaagcata tgatagccaa atgattacca atcgttggag taccatgcgc
     7441 tggatgatcg ccgaaggaaa ttgtgattgg gatacagtag ttcggtattc gaaaaatcat
     7501 ccaacgagcc gtaccaggat tgtgttgaat gaaatgttta acccgatacc gagggtacat
     7561 gaggatattg agtctcaaca gaattctcca cgagttgtcc taaaataatt tatgtcaata
     7621 ttttgatctt gaaccttcga cagacgccct ggcggtctgc gaaggtttat aaaacatctt
     7681 gagtatttcc agatgattgc caagtacaga tagtcttttg cttcatttca atatacaagt
     7741 cctgaagtat ctacttgctt aaacttcata taccccataa aatcctaaat tcatttgaaa
     7801 ttgctaggag tagtacatgg ggtggaatga gccagagaag ggcaaagatc catggtcggg
     7861 taaaaatcaa cctccggatt tggatgaagc tcttaaacgt atccaggata aattgaagaa
     7921 aacatttttt ggaggcacgg gcaaatcaaa taataattcg tcaggtggtt ccagtggcgg
     7981 gttgcttgct gtcacagttt tattaatagc ttttattctt tgggctcttt ctggtatttt
     8041 tattgtggat cccgcagagc aagctgtgat actccgcttt ggtaaatatg cagaaacagt
     8101 tggccctggt cctcattgga ttcctcgttt tatctcatca aagattgtta tgaatgtgga
     8161 tagggtgcta gattactcct actccgcgca aatgcttacc agtgatgaaa atttagtatc
     8221 ggtctctttg gctgtccagt accgcattaa tgatttgagt gaatatttat ttaatgttgc
     8281 aaatcctgag gaaagcttac aacaggcaac ttccagcgct ttaaggcaag ttgtaggtac
     8341 gacaaccctg gatcagataa taactgaagg tcgagaagtt tggggaggta gagtacaaga
     8401 aactctgacg aaaactttag aaagctataa gaccggaatt ttaatcgtta atgtgtcacc
     8461 ccaacctgcc agagctccag aaagtgttca ggatgctttt gatgacgcga ttaaagctca
     8521 agaggatgaa aaacgtttca aggaacaggc ttatgcttat gcagcaaaag tggtgcccat
     8581 tgcagaaggt aaagcgagtc gtattcaaca ggaagcagaa gcttattcga agcaagtcgt
     8641 cttaagggca cagggagaag tagcggaatt tttagcctta cttcctcaat acaatgcagc
     8701 tccacaagtt acggctaagc ggatgtatct tgaagctatg caaaaagtaa tgaataagag
     8761 cagtactatt attgttgaca gtaaagcagg aaatttattg tatctacctt tagataaact
     8821 acttggtaat caatacgttc cgcaatctga aaatctaaaa gccataaaaa atggtacgaa
     8881 tgtcagtaat gaggaggaca acttgattct ttctggaaga gatctcacaa gaccaactta
     8941 tagtcaaggg agagattgag aatgaatgca tttaaaacga cattgggtgt cttaatcttt
     9001 ttggtactag tgcttttgtt taccagtatg tttactgtca cacaaggaca gcagggtatt
     9061 attttacgtt taggacgttt ggtaaaagac cctcaaactg acgcagtgaa agtattaaat
     9121 cctgggctcc actttaaaac cccttttatc gaaagtgtgc gaattttcga tacacgcatc
     9181 caaaccatgg atatcaaatc gacccgtatt gtgaccaaag agaaaaaaga tgtcatggtt
     9241 gactattatg tgaaatggcg aatttcagat ttagcccagt attttaaatc aacaggtgga
     9301 aatgaattta aagctgaaac gttgctagaa caacagttaa ataccttgct cagagcccaa
     9361 tttggtaagc gtaccatatc cgatgctgtt tcaggagggc gtgatgatgt tatggaaata
     9421 ctgcgtaatg ctgctgagaa gcaagctggt gaattaggta ttaaagtggt tgatgttcgt
     9481 attaaaggta ttgaattgcc ttcaaatacc agtaatgcta tctatcaacg catgagagcc
     9541 gacatgcaaa aaattgcaaa cagacataga gctgacggac aagctgccgc agaacagatc
     9601 caagcgaaag ctgatgctga tgttacggtt ttattggcta aaactaaaag taatgctcag
     9661 cgaattagag cagttggtga agcggaagca gcagctatct attccaaagc atacactcaa
     9721 aatccggatt tttttgcttt atacaaaagc ttgttagcct atgaagccag ttttcatagt
     9781 aaaaaagata ttttaatatt ggatcaaagc agttcatttt ttgattattt caaacaagct
     9841 atgccaaaaa atgatggtac agcggctaaa aagtaattat aatgtgaaat tttgctgtaa
     9901 tcaggttcga tatttattag catcatccat ctaatataaa tgcagttggg tttctcgtaa
     9961 aatgcaactg catttttaat ttttacaggt atgcaaacaa agagtaaatc atgggtaaga
    10021 atgttgttgt tttgggtaca caatggggag atgaaggcaa aggcaaaatt gttgatttgc
    10081 tgacacaaga tgctcaagtg gttgtccgtt atcaaggggg ccataatgca ggccatacat
    10141 taaaaatcaa tggagtaaaa acagtacttc gtttaattcc atccgggatg cttcgcccta
    10201 acgtaacctg ctatattgca aatggtgtgg ttttatcacc tcaggcatta cttagtgaaa
    10261 ttaaggagct tgagggtaat ggtatcaatg tgagggagcg attacgaatt agcctggctt
    10321 gccccctgat tctcccttac cacattgctt tggataaggc tcgtgagacg catatgggca
    10381 agtctgcaat tggaacaaca ggtcgtggta taggtcctgc ttatgaggat aaagtggctc
    10441 ggcgcgcttt aagagtaggc gatttatttc atagagaccg atttgccaat aaattaacag
    10501 aattattgga ttatcataat tttgtattaa cacaatattt caaacaacca gctgttgatt
    10561 tggaatccct gttggatgag tcattacaat gggctgaaga attaagacca atggtttgcg
    10621 atgtttctgc ctgtttgcat gaacatagaa aacaaggtga aaatatttta ttcgaagggg
    10681 cacaaggtgt ttatttagat attgatcatg gaacataccc ctacgtaact tcttcaaata
    10741 cctgtgtggg atcggtaatt aatggtgcag gatttggtcc tcgctatatt gattacgtgt
    10801 taggcattac aaaagcttac acaactcgag ttggcggtgg cccattcccc actgagttac
    10861 tggatgacgt tggaaaacgc attgcggaac gaggacagga attcggcgca gtaactggta
    10921 gacccagacg ttgtgggtgg tttgacgctg ttttattaaa acgctcgatt gagttaaata
    10981 gtatttcagg actctgtgtt accaaattgg atgttctcga tggtttagag gtattacgga
    11041 ttgctgtggc ttataaagac agggatggca atatattatc tcgacctcct ctggcagctg
    11101 atgatttcaa cgatttgtta ccagtctatg aggagcttcc tggttggcag gaatctactg
    11161 ctgatgtgac tgtgatgagt gacttacccg ccaatgcaag ggcttatcta aagcgaattg
    11221 aagagatatt agggattcct attgatatgc tatcaacagg acctgaaagg gattcaacaa
    11281 ttactttacg aggtcctttt ctttgataca aagataagtt ttgtaaccac aatgagtgtc
    11341 atgcaaggat ttcttgatat gacactttct gccttttaag tattattgtt ctgtgttttt
    11401 gcaagctatt tccttggggg ccgcaggcaa ttcttgcatt gctaaatttc atttcatgct
    11461 aaagttaatg tacataaaag ataaattatc tcttatctga atgtttactc aataaaatgg
    11521 tgaatcatgg aatatctaac gcccagagga ttgattcctt ttataaccga actcaaagcg
    11581 cttaatattc tgcaacaaga tgataatact aatattgctc tcgtatcaga aattttagca
    11641 cgctgggttg tagataaaga ctggctggat gaaaaatttt actatgttaa tccagaggtt
    11701 ggctttaact cctggttaat tcatgaagaa aaagatcata ctttagcagt taatatcgtt
    11761 gcctggcagt caggacgaga aattactccg catgatcacc agacttgggg tgttgtgggt
    11821 agtataatag gtgttgaaaa aaattatttt tggaaaagaa tggatgatgg ttcaaagcca
    11881 gggtatgccg agatcataag agaaaaaacg gctattatac gtcccgctgg tggggtaatt
    11941 ggttttttgc ctaatgacat tcacagtgtt atgaatgaat cagatgatgt agcaatttca
    12001 ctgcatgttt atggaaaaaa tttgaattat acaggtcgcc atcagtacga cccggttaac
    12061 aatttaatgc aaccatttat agtaaattat agttaggttg atatttatca gtgtcattat
    12121 gctttcaata ctgccaaatc gccgtttatt aacgcaggtc ttgtaaattg atattaaact
    12181 ctttgttaaa acatattttt aattctgtca tttagtttat ctaaattccc tcattaaaaa
    12241 catgttttac ttcgtgcttg ggtttttcat aagccctgta aaattaaaat tgtagctacg
    12301 aagcaaataa atatggaaat tccttcatta aaatatcaag aactttttgt tgatcagatt
    12361 tacttttaat aatgagcaag accgtgtcgt ctccggcaat agttcctaaa attcctgaat
    12421 ttgcagagtc ttttatgctg aaggaaacgt atttacggtc tatataataa gcaagactgt
    12481 ttgcattacc aggatgagtt tgcaggatga ttagcccaaa atcagaaatc tggacattta
    12541 gaaccagagg taagccaggc tgattgtaat caatgatttt gtaataaccg gatacttttg
    12601 caattttcat tttttttaag cgacgagaaa gggttgcttg aggaatctca taccctcttt
    12661 tttttaagct gttctgcaaa tcactttgtt ccgcaatact ttcagtttgc actattgaca
    12721 aaatcaaatc atccaaaaag gcatcatgag tcaattgctt tctccctgtt atcaattaaa
    12781 ttgaatataa attactaaaa ttgaaattat atccattttt tcttgacagt acaaatcctg
    12841 atcatgtata ttcacaaaac gaataaatat cggatacata ttcatgaaaa agatttgtct
    12901 tttgttatta atcagttctt ttatacttat atccagttgt agtgagcaaa aaaatcaaaa
    12961 tacactgcgt ttttccactt cagcagaata tccacctttt gaatatattg aaaatggaga
    13021 aataaaagga tttgatattg atttggcaaa attgattgcc aaagagttgg gtaaagaagc
    13081 cgtttttgac aatatgcaat ttagtacggt tttgcctgca ttgaattcag gccaggatga
    13141 cgttgcgatt gccacaatta ctattaccga ggaacgtaag aaaaattttg atttttccat
    13201 accttattat tttgaaggaa tggctgccgt ttttgatatc tcacaaccag tgaaagagat
    13261 ctcgcaatta acaggaaaaa aaatagcagt ccagctgggt agtgttatgg aaatttggct
    13321 tcatcaaaat tttcctaatg ctgaaattac ggctttggat aataacaacc aagcgataga
    13381 agctttaatc gcaggtcatg ttgatgtagt ccttatggat ggggcacaag ggggtgttta
    13441 tagcaagaaa tatccaggac tgtcttattc catcctggcg aaagcgaatg aaggttatgg
    13501 attggtctta agaaaagatt ctcctctaag ggatgaaatc aattccgcat tggaaaatct
    13561 taaagcgaag ggtgaaattc aaaaattgga atccatttgg ataaaggggt caatatgacg
    13621 gatgtgatgc aaaatctgtt tttcattggc aaaggaatag ttctgactat gcaattatta
    13681 acagggggta tgctgattgg agtattgctt ggaactctct tgtcaatttg cagacatcaa
    13741 ggagtcgctt gctttgttat aaaccgattc atatccatat tgagaggcac accactcatt
    13801 ttacaattaa gttttattta tttcgcagtg cctgttttat taggtatgcg accaagtatt
    13861 ttagtagctg gaattgttgc ttttggtttg aatagttcgg cttatttcgc tgaaatttta
    13921 agagccggaa ttgagagttt acccaaaggg cagtttgaag cagcccttac tctgcaaatt
    13981 cctacctttt acttatggaa agatattatt ttacctcaag tggcaagaaa tatttttccc
    14041 gcaatgatca atgaaatgat cgccttgtta aaagaaacgg ccttgatttc tactattgga
    14101 ggcatggatt taatgcgcca ggctcaaaca ttggcagcac aacaatttac ttactttatg
    14161 cctttatgta tcacgggttt ctattactac agtttggtct tactccttga agcgctaggt
    14221 aaaaaaatcg aaaaaagggg acattatgct gcaagtcatt aacgcatcga aaaaaatcgg
    14281 caatgcccaa gtacttaatg agattaattt aagtattcaa gcaaacagta ttgttggttt
    14341 agtagggcct tctggtagtg gaaaaacaac tttactgaga tgcatacaac aattggagtc
    14401 aatcgattca ggctctatcc agtgcgaagg aaaaagcgga tttatgtttc aggattttca
    14461 attatttccg cacatgacag tactcaataa cctaatttat gcaccaagtc ttcacgataa
    14521 aactgctgat catatagcaa acgcgtgggc tttacttgct atgttaggtt tgtcagacaa
    14581 agctcattct tacccacaag gattgtctgg tggacaaaaa caacgcgtgg ccttggctag
    14641 aagcttaatg atgaaaccgg atttattgct ttgtgatgaa ccgacttctg gtcttgatat
    14701 ggcgaccact aatgatgtca ttgctttgtt gaatacagtg aagactctaa aaatcactat
    14761 gcttatcgct tctcatgatt tgtttttttt atccaatatt gcggagcgat tgttggttgt
    14821 aaagcaaggg caactaattg ctgactttaa aacccaagag gtgcctgatc ctattttata
    14881 tctacagcag ttttaccagg gggaaatatt atgaaaaagg ttattaaaaa aatcgcatta
    14941 gcttattcag gtggcttgga tacctccatc atgatccctt ggcttaaaga gcattatgag
    15001 catgctgaaa taatcgctgt gatttgtgat ttagggcaac aggaagatct cgatgccata
    15061 aaaaataaag cgctaaaaag cggcgcttcc aaagcgtatc tagtcgatgt gaagaatgag
    15121 tttgccattc aatatctttg gcctttggta aaatcaggtg ctttatatga agatcaatac
    15181 attttgggca ctatttcaag gccactaatt gctcaaaagc ttgttgaaat tgctttgatg
    15241 gagcaagtaa atgccgttgc acatggagcc accggtaaag ggaatgatca agtgcgtttt
    15301 gaatattcgc ttaaggcact tgcacctcag cttgaaatta ttgctccctg gagagcttgg
    15361 gatattaaat caagacagga agcaattgtg tatgctaaag cgcatggcat tgaggtacca
    15421 gtgactccca aagcgcctta ttctagagat cataacattt ggtatatttc tcatgaaggt
    15481 ggtgtgctcg aagatccttc tcaggagatg cctgatgatg tgttattaat gaccagtcct
    15541 gtatcgcagg cccctgatga agaggaaatg attattcttg atttcaaaaa aggggttcca
    15601 gtcgccttaa atgaacaaaa actttctcca gttgatctct taaatacatt aaatcaaaag
    15661 gcaggccagc atggtattgg agtggcagac attgttgaaa atcgattagt tgggatgaag
    15721 attcgaggga tttatgaagc acctgctgct gcagtgcttt ataaagcgca taaactgctg
    15781 gagagtttat gtctcactcg ttcaacgtta catttgaagc aatctttgca gcaaacttat
    15841 gccaatttgg tttatgaagg aagatggttt tcacaaacca agcaggcttt ggatgcattc
    15901 attgatgtaa cgcagcagca tgtcacgggt cgtgttaagt taaagttatt caaagggaat
    15961 attatccctg ccggtatgca ttctccttat agcttgcatc atccagcatt agctactttt
    16021 gaagaggata atgtatacaa ccaaaaggac gctgaaggat tcattaacct cttttcttta
    16081 tccgctaaaa tttatagcca ggtacatcag ggggggaatt atgactaata aaacatgggg
    16141 agggcgattt aagaaatctt tggatagcgg tgtcaaccag tttaatgcgt cattgtcttt
    16201 tgatcatgtg ctttttgacc aggacattaa tggcagtcag gttcatgtga agcaattagc
    16261 taaacagaag attttaacag aagctgaatg ccaggggatt tattctgctc ttgaagaaat
    16321 cagaacagag ataaaacaag gccaatactc atttaatgaa cgtgatgaag aagacataca
    16381 tatgtttatt gagcaattgc tgattcaaaa aataggtgat ttgggtaaga aattacacac
    16441 cggccgtagt cgtaatgatc aagtggcttt ggatcttcgt ttatatacca gagataaagg
    16501 ctgtctgatt aatgaacttt tgacaaggct tatcgtttgt ctagatgatc tcaccagtaa
    16561 acatcaacag gatttaatgc caggctatac tcatttacag caagcccaac ctgttgcgtt
    16621 aggggcttat tttaacgcgt atcaatgcat gtttagccga gataaaagca ggttggaaga
    16681 ttggtttaaa cgcatgaatt attctccttt gggcgccggg gcactggctg gaagtacctt
    16741 gcctcttgat agagaatggg tagccgaatc cttgggattt gcagggatca ttcctaatac
    16801 tcttgatgca gtcagtgaca gagattttgt catcgagtta tgcagtgttg ctgcaatgat
    16861 tatgatgcat ctctccagat tatgtgaaga tttgattctc tggtctaccc aggagtttaa
    16921 ttttgttaca ttggatgatg cgtttgctac aggctcttca ttgatgccta acaaaaaaaa
    16981 tcccgatgtt cctgaattga ttcgtggcaa atcaggacga gtttatggtc atctaatggc
    17041 catcttaaca gtaatgaagg ggttgcccct ggcttataac aaagacatgc aggaggataa
    17101 agaaggatta tttgatacga ttaatacgat aatcgtttgc ctgcaaatga taactccttt
    17161 tttgcaaagt ctgactttta ataccccgtt aatgagaacc aaagctcaaa gtggttacct
    17221 tgatgcaact gccattttgg agtcattagt gatgaagggt atgccttttc gtgatgcgca
    17281 ccatcaagtt ggggcatgga ttgcagaagc gatagaaaaa caatgttcac tgaatgaatt
    17341 attaaaagga ggctaagaat gtctttttcc acctcaacca atttgactcc aattgaaatg
    17401 caagacgatg accttgaaaa aattcaatct cagaataacc catctgcaat taatgaagta
    17461 aagaccaata cttttgtcat taatttacaa caagctattt ttggcctgga gacgggtagt
    17521 gaggccattg attttgtttc tcataagaat acaggctttc catttcctgc tggtgaatac
    17581 aaaccaagac attttcttac tggcacagag ttagcaaggg aggaaattca gtttatttta
    17641 agccaggctc gctctctaaa acaaaacccg gggttatata atcaggcact tgctaataaa
    17701 agtttggtaa tgatttttga aaaaccttcc tttagaaccc gtttgagttt tgctttagcg
    17761 attcagagta tgggggggct cgttgttgaa agcatcgcta actcaagaaa gcaggagact
    17821 ccagctgata tggctagagt tttaaatggc tatgctgatt ttgtaatggt cagaacacat
    17881 gaggacagtt ttttgcaaga aatggccgaa tattccaaaa tccctattat taatggcctc
    17941 tccgcattgc atcacccttg tcaaactctt gctgacttgt taagccttga agagcaattt
    18001 ggtgctttgg aaggattaac cctcacttac ataggggatg gcaataatat tttgcatagt
    18061 ttgatgcttt tggcaccgca gttaggtgtc actgtgcatt attgttgccc aaaatcaaat
    18121 caacctaatg aggctattgt taaggaggct caaagcaagg cagggaaaca aatgatttat
    18181 tattttgata aaccagagga ggccgtgaag caagcggatg ccgtttatac tgatgtatgg
    18241 acaagtatgg gatttgataa tcctgaagcg ggcaagattt ttaaagggtt tcaggttaat
    18301 gaaacattaa tgtctcatgc aaaacccgat tcggtgttta tgcattgtat gcctatggaa
    18361 aggggaaaag aggtttctgc cactttaccc gacagcgcgt catctatcat ttttaggcag
    18421 agtgagaata ggatgcatgt acaaaaggcc ttgatgctat ttttggatca atagaacctt
    18481 gagttatgag gcccatatta gtgtataatg aaacgctatt gcgcatttct cctgatatgg
    18541 gctcattatt tttgcaaatt aatttagtaa ttcttctcaa aggcgattaa ttgttgttct
    18601 aggctccatt gcaattttgc tttttcgttg tgttcgataa atttttgaca gcttttgctt
    18661 ccaaggtcat ggcaatcagg aatcaattca gctttatccg cattagccca tatacttttt
    18721 ccaggtaaga aggagtaagt taatgtattt ctgttaattt gttttaattg ctgataatta
    18781 aggccatgct ctttggctgc ctctacatat tgttgggtta agtccgttct tagcactcct
    18841 tcgtcatcag tggataaaac gactggtaca ttatgagtaa gataataatt aagaggatga
    18901 ttgcggccag atatattcag aatttttaaa ttactaatca aattgatttc aacgggtatg
    18961 tgatggttag ccatgtattt taaggtatct tctgcattgt tttcataacc gatgtccaca
    19021 ccgtggccaa ttctttgggc gtgtccaatc agcagtgcat cgcgaatatg attacttaga
    19081 ttttctggtg tcactgcctg aggtgataat tctccggcat gtaaggtaat aggaacattc
    19141 gggtatattc gatgcagata ttcaaagatg gacatgtgct tatggtaatc acgcaaagaa
    19201 ataattccat cttccggttg cacgaggttt acgccgatta agttcccctt agacttggag
    19261 acggcttcaa aggcattcaa ggcttgggca aagacattat caacgggctg ctctctgagt
    19321 acataataaa taaatttaat ttttacatgg caggaatcgg aagaagggtt atctttgcag
    19381 cctaattctt gagatgcttt ggtaagaatt cggtcacttt ccaaaacagt attttttatg
    19441 ttgctttgaa aatctttatt cgctaacaac aggcgccttt tctggtcata tgatggaatg
    19501 tccttgatta aatccccata agttaatgac tgggcattgt ccgggattat cataatttcc
    19561 atgtattgct ctttttgctg tgctgctcgc tgaacaatat cggcaagcaa ttgaggccta
    19621 taatcaaaaa ctatgggcat atatttgaca aatccattga aaaaatgatc gtggccagat
    19681 tctttaccag gtataaagtc tttcatggac cagtctttaa ttatgttgga atagagttca
    19741 ggattgttaa aaacctcctt aacattaact ccctcacaat gagaagaatc tttacttacg
    19801 gcaaatgaga gtttatcgag gcaatagttg ccatttgcgg caagtgccag catggtctca
    19861 ggataggctc ctccagccaa atgataatgt aactcgccac ccttgggcat tgttttaaaa
    19921 aaggcataaa gggcactgga atcctttttt atatgactaa aataggaatc aacactggca
    19981 taaattgggc acacaaggaa gaaactggta aaaaatgcga gtaatctggt aatgttcata
    20041 atttcaatgt gatcattaat tgggcagata ttatctcata atcttgcacg attcccaaca
    20101 gaatatctgt tttatcatgc aatctcttat tattttttac gaaatgataa tgattgtatt
    20161 tttttattat tcattgcaga atgtttttaa ggtttaacac atggaagaaa aaatgcccta
    20221 tttctataca agatcggcac ctttgttttt tgtattcgtt attgcactta tttttataaa
    20281 tttttcctat gcccatgaat ttcaaactga ttcaaaaaat aaacataaag tggatgttca
    20341 cataggtatc tatgctccgt tttccagtca ttcagctttt attggaagaa atattctggc
    20401 tgcaatggaa atgggcagcg aacggttaca gtcctccgat attcattatt cattttatac
    20461 tctggatcaa cttcctaaaa ataagaatgc tggtcttgtg ctgcaaaaat ttattgatac
    20521 ccatcatatc aatgtgttgg tgactggagg taaagaaacg gggttaatag cagctcccat
    20581 agcaaagaaa aacaatttga ttcattttag tctgaccaac aatactgaag ttgcagacgg
    20641 cagaaataat tttatcgtct ggagtccagc ttatgaccaa gccaaggttt tggttaacga
    20701 actaaaacgt cagcaggtcc atcacttggg aattattacg gcaaatcaac cgtcctcaac
    20761 cgctttgaca catagtgtta taaaacaatt acaggctgac tctcatattg atgtggctgc
    20821 ctccgagtat tttgaacctg gaaataagga tttttcaaga ttaactaaca gaattaaaag
    20881 aaaaaatgca gatatgtatt tagtaatggc atcacctgag gatattaaat taatacaaga
    20941 acaaatggag gcagataaac agcatgtgcc cattaccagt atagttgagc gtgctactcc
    21001 agaagtggta aaggtatttg aaggtcaatg gtatgttgat tctcatgaga tgcagcctga
    21061 ttttgttaat gaatttaaac aaaattattt aaattatcct gtaactgagg caggttacgc
    21121 gtttgattta tttcagcttt tgaacaaaag catagtaatg gcaataagaa cacaaggttc
    21181 tcctacaagc caggaagtcg ttcatcaact ccatgatatt gccaatggaa caggcgtaat
    21241 gggaactatg gtattggaca gtaagggttc tttatttacc aaatctgaag tgaaaactgt
    21301 gaaaaatggc gagattcata ctgtctaggt agaaaataaa gcctgaaatc atcagctcat
    21361 tgtgaatcga tgatttcagg cgggagtgac taattggtca ttatctgtta gctaacgcca
    21421 ttccttccaa tacggtgagt gccgcatata actgataatc ctcgtgtatt aaatctccgg
    21481 tttgaatttg gttgtttttg gattcagtgt tttctgaatt gtctttgtta atcaagtgcc
    21541 cattaaggtc cgcttcagta aaaccagcca gagtgtcttt tttgactgca tttttaggaa
    21601 cgtctacttc ttctacaatg atgtcaggaa ttattccttt ggcttgaatg gagactcctg
    21661 atggagtgta ataaagtgct gtggttagtt ttattccggt tttgccatca agaggtaata
    21721 cggtttgaac ggatcctttt ccaaaactct ttgtaccaag gattatagct cttttattat
    21781 cttttaatgc cccagcgaca atttctgaag cagaggcaga accattatta attaagacaa
    21841 cgataggcgc attgtctaac acatcccctg gattggccag ggcggtgaat ttagagccag
    21901 gtagtctgcc ttccgtatag actatcattt cttgcttacc tgccttgtca ttgccaagaa
    21961 aagcatcgga tacctggata gctgaatcaa gtaaaccccc ggggttattt cttaaatcca
    22021 atactaaccc tttcaatttc ccgcctgctt gttgttttaa ttgttcgatt gctttaatca
    22081 tgtcttttcc agttaaagcc tgaaattgag tcaatcgaat gtaaccataa ccttcgctta
    22141 gcattttgct ttttacactt tttatttgaa taacttcgcg aattaagtca aaagttaaag
    22201 gtttatttac accttttctc aagatagtta attctatggt agtacctggt ttgccgcgca
    22261 ttaaattaac agcgtcttta agagaaagac cttgaactga ttctttaccc agtttaataa
    22321 tataatcgcc agatttgatt cccgctttaa atgctggagt gtctacaaga ggagtgatta
    22381 ctttaacaac accttcttcc atggtcactt cgatacctaa accaccaaat tcaccgctgg
    22441 ttgatgtttg caactccttg aattcctctt cattgagata actggaatgc ggatcaaggc
    22501 cggttagcat cccgcgaatt gcgttatcaa ataattcttt atcatcaact ggtttgacat
    22561 agtatttctt tatttcaccg atggcattgg aaaaccgttg cacatcttcc aacggaattc
    22621 gttttgtatt agaataattt gaattggttt cttcagccga aaacgcagtc aaaggcagca
    22681 tcagagttag ggcatacacc aatgccaaag tactggaaaa aagtttctta ataaccatag
    22741 cgatctcctc agatgcaagg ccatgaaatt agccacctgc gtattttttt atgcaattta
    22801 aatgccagcg actaagcttt aagacaacca gtctaacgga ttgaccgctt ttcctctttg
    22861 tcttatttca aagtatagac cgttttgctt aattccaccg gtatggccta cacttgcgat
    22921 ttgctcgttt tgatggacga tttgtccttt gcgtttgaag agagattgat tgtgtgcgta
    22981 taaagtcata aacccttggc catggtcaat aatcagaagc aaaccataac cttttagcca
    23041 atcactaaat acaactttcc ctgggtaaac ggcagtaact atggagcctt catcggcaaa
    23101 aaatgtcact ccttgattca tcctgcgtaa agatctatgg gggctttgga caggtaacgg
    23161 caattttttg cgcattcccg tgaacggttt gccgctttga attacacttt gttgagataa
    23221 tgacttcatg agatgagata agttgtctct atctttttga aaatctttta atgattgttg
    23281 attattttga atgtcattat caatggattg aatcagtgtg ctatggtatc ttttattttg
    23341 ctctagctgt ttttgatttt gtgacaactt aaattgtagc tgcttgtttt ctgcaagttc
    23401 atttccaagc ttggttttac tgtcgttaag attttgtcgg gttcgagcaa tttcatcaat
    23461 caactgctga cgtgatctta ttatatactg ataataagtt aaaattcggt tgatcttaaa
    23521 aggctcatct tggttaatca accatttcaa aggttgatac tcccccatct gataacgtgc
    23581 cctgacatga tttgccagca tttcctgctg agcttgcaat cgtttattta agtgatttat
    23641 tttcccttgc agttcggcaa tacttttttc ttttgcggac atatttgctt gtatgattct
    23701 tagcttgcgt attccttctc caatttgttt ttcagtgctg cccagctctt ggtttagaac
    23761 agtccgttta tcctgtgcat tggctaaaac ttgctttagt ttgttaatct tggcatctaa
    23821 ctgttttagt ttggtttgtg cttgagttat agaatttgga gcagctttta cctcaaggcc
    23881 attacaaaaa aatagaatga ccataagtgc caaaccaaga gaagcaccgg ataaagagtt
    23941 ggataggcta ggcatgcgca tggttttttt ccagaagaat acgaccagtc atttccggag
    24001 gcggggttat ccctagtaat gcaagcaaag aaggtgctat atcgattaaa cttccttcgg
    24061 aatgagtgaa gtgccaccct ccacctacat aaagaaaagg aaccggttca ctagtatgtg
    24121 ctgtatgagc ttgatttgtc gcttcgctaa acatttcctc tgcatttcca tgatcagcag
    24181 tgatcaataa tttaccccca ttgtttttaa gggcttgcca aacctgctgc atgcactgat
    24241 caaggcactc aatagcttga acagtagctt caaaatttcc cgtatgtccc accatatcag
    24301 cattggcgta attacaaata atgacatgat aatcttggct attgatggcg gctattagag
    24361 ttttagttaa ttcatgcgca ctcatttctg gttgcagatc ataagttgcc acttggggag
    24421 agggtaccat aatgcgctct tcatttgtaa aaacactttc acatccgcca ttaaagaaaa
    24481 aagtaacgtg agcatatttt tcagtttcag caatgcgtaa ttgactgaga ccatgagaag
    24541 acaagacttc acccagggta ttatttaaag gaacaggtgg aaatgctgtc gtggtaaata
    24601 aatttttatc atattgggtc atggagacaa aataggacag ctttggcagt gtttttcttt
    24661 caaatccttt aaacgaagga tcaaggaata tagaggttaa ttgccttgct ctgtcagcac
    24721 gaaaattgaa aaacaggaca gcatcaccat cttgtataga atgctcttcc cctataaggg
    24781 tgggtggaac aaactcatca gaaattttat ttttgtaata aaaatgtatt gcggtttctg
    24841 catcaggaaa ttggcgctca ctttttcctt gtgttaacaa attataaaca ggctctactc
    24901 tctgccaacg tttgtctcta tccatcgcat agtaccggcc acaaattgaa ttaatggttg
    24961 caacagggtg tttagccagt tcttcattca ggcatttcaa gctatcaaga gcactttgtg
    25021 gtggggtatc tcggccatca agaaacaaat gcagatggac ggatctgaat tttttttgat
    25081 tacaaagggc aagtaaagca aacaaatgtt gttcgtgact gtgcactccg ccgggagaga
    25141 gtaaccccat gatgtgaaga gatttttctg ttttctttaa ttggtcaatt acttcatgga
    25201 aaacagcatt tttagcaaat tttccgttat ttatcgcttc attaatgcga gtaaaatctt
    25261 gctgtatcac tcggcccgca ccaatgtgca tatgcccaac ttctgaatta cccatttgct
    25321 catcaggtaa accaacaggc aagccggagg ctttcaaaag aatatgaggg caggtttgcc
    25381 accattcatc ccactgaggg gtattggctt ttgcaatagc attgtatcta tcattttcgt
    25441 tatagcccca gccatcaaga atcatgagta ccaggggagc attatgcgac atgacctaaa
    25501 tcctaaattg tacaatgaaa gggctaaaat gataattata ccttcgcggc agtaagaggt
    25561 atatagtttc agtaagagtt cgctgttatt aaatttagta gcaatcttct ttttgttcgc
    25621 agtgaccata agcatcgcat gactggatcg gtcgacaccc tgggtaataa ataatgggta
    25681 caggagttgt aataattaaa ggaggagtag ttgatttatc acaggactta caatgattaa
    25741 aagtagctct tacatcattg tctttgggta gattttttgg atttggtcct atccctaata
    25801 gattcatcgc catggcatgg ttaacaagag cagttaagct gacaaataaa ataattgctt
    25861 tgaggatcag gttcttagta tccctactca tgatgtgaac tcctttttaa ttttatgcct
    25921 cgtattatgc gataataaga gtatagttgc ttgttatggt tctgctacta taccaaagga
    25981 ttatcttgca attcttccgg tacatattga gacaaatatt tacttaaact caattgcacg
    26041 gtcgaggtta tcccatattg cgtttttaac aataataaag tactccagcc ccaccaactg
    26101 tttgtccaat gcctgtcata aggatttgtc aaattggttc ttcttaacaa ctgtttgttt
    26161 tctttatccc aatagaacat aggagcaata tccatgttat tttcatcatt taattttgtg
    26221 atatgtggaa tcatgtcctc tatttgattt ttttctggta gaacgcctga gtaataagct
    26281 tcaaatattt catcagcgga agcttgcaag gcctttaaga gcatctgctg gttggtttga
    26341 ttaaaaggat taaaataaga atagtcacca taaaccatcc attgttctcc cagattattc
    26401 gtcacatgaa ttccatattt attttcttca ttatgcatat aattggataa caacgcacca
    26461 aggactgttg gtgtgataat ggctgataat tcttctctag gggtgcgcaa atggccggca
    26521 gcgaagtgat ctgataaata gtggcaggca aaagcatcca tcgcataggc gatttccaaa
    26581 tcagagcgtt ttcctgttcg ttttgctttt aatgcctgtt gcagtgcgac atggtggcca
    26641 cttttatagg ctatcagatt atttggcgta aagtgatcat agttttccat ggctagtttt
    26701 agatatctgc caggatttaa ccaccaggtc aggctattac aaccacctcc cgtgatgcag
    26761 ttgatttggc gacctacttc atttccaatt cgtttataaa tggcttcagc agtttctcct
    26821 tttgcaatcc ctgattcgac ttcgcgcatt tctgctctga taacggaatt aagatcttct
    26881 acctcactaa ctgcaatggc attcctggca aaactgttga aaacctcttt aaaacgagtt
    26941 tgtttttcca gttttgttaa gccatgacta attggttttc ctacaattcc atacagatca
    27001 ccaagactta tgatatcgcc ggctgttagt gccaatccat tttttaaatg taatacaaca
    27061 cctgattggg gaacctcatt tatgtcaaat cgtagtttga tttgttgccc catggaccaa
    27121 tgctcggaca aagcaaaact gccgttcgat tcaccataag ttatagagga aaaagccaac
    27181 aagctaatgc tcattgcaaa taaccggggc tttactaatc gacggataga ttttttcatt
    27241 atgtcctcat caacagggat tgatcattgg ttttagtgag aaatagcata ttgtataacg
    27301 tttgagatga aatgttgatg cattggttcg tcccgagata atgcataggt gtgaatggag
    27361 tctgtacaaa taaccgtaac caaaaacgac atagagcgct ataggtcatt ggaattatct
    27421 cctttctgga tattcatgat gaaaagtgac aactcgttac gaagatttat tcaagtttaa
    27481 taatgagttg tttcataaac aaggtcaatt gatttcttaa gtagtactta aatttttatc
    27541 tcaaagaggt tagactatgc atcattttta ttagtcggat aagttgtttt gaatcataaa
    27601 ttacctcaac acattgccgt tgtcatggat ggaaacgggc gttgggctga aagccgcggt
    27661 ttgccccgtg tagagggaca caaagcgggc cttgactcag ttaaaaaaat tatcaattgt
    27721 tgcctggaaa aaaaaatttc ctgcttaagc ctgtttgcat ttagttctga aaattggtca
    27781 agaccagtga cagaagtcaa ttttcttatg gagttgtttt tggaggcttt gcgaaaagag
    27841 atagacgatt taaaccagca tggaatacgg ctaaagttta ctggcgatag agaacctttg
    27901 tctcaagtat tacaaaagca aatgagtgat gcggaagctc ttaccaagaa taatcaacaa
    27961 ttaattctca atgttgttgt gaattatggt gggaaatggg atatagtgac agcggctaga
    28021 aaattaatac gcacggtttt ggatgggaag ttggcatatg atgaaatcaa tgaagcagta
    28081 ttcgctcaat ttcttgatac gaatggcatg ccggaacctg atctgtttat tcgaactagt
    28141 ggtgaattaa ggataagtaa tttttttctt tggcagttag cttatactga gctgtatttt
    28201 actgatgttc attggcctga ttttaatgag catgagtttg aattggcatt gatttctttt
    28261 gccaagcggg caagaaggtt tgggcaaatt tcacaatctg aataaaggta aaaccatgtt
    28321 tatgcaacgt ttgattacga cattaatatt agcgcctttg gttttgcttg ctattttcta
    28381 tgcgccttcc tggattttat ggggtgttgt ttttgtgatc ctcctgcttg cgggaattga
    28441 aagtttgaaa ttgattcctg ttgacaattt atctatgcag gcttgttttt tgatatttct
    28501 attgatgtgc ttttgggcat gtggctattt atatccttat tggttaagcg tggggttatt
    28561 cgtttggcta ttcaatggct tggcaatcct cacttatcca cgctcacaaa attattgggg
    28621 ctatccaatg attgttgcaa tagcctgttt gatattgtta cctttattta ctcaaagtct
    28681 tattcaaatt tcttttttac agcaaggaaa ggaattactt gtctatttgc tttttttaat
    28741 ttgggcttct gatattggtg cttatctcac tggaaaacaa tggggaaaac ataaattaat
    28801 ccctaaagtt agtccgggta aatcttggga aggtgttaca ggcggggtta ttttagccat
    28861 gatagtcgcg actttgggct attattattt tataccttac tcaatttata tatggtttgg
    28921 tttagcattg tctactgtag ttatttcaat ttttggtgat ttgttcatca gtatattaaa
    28981 acggcgttgt caattaaaag atactggaaa tattattcca ggtcatggcg gtatattaga
    29041 tcgactggat agtctaattg ccgcgttgcc atggttttac tttggattaa cctacatacc
    29101 actgggaatt taattttctg gtcgtatcaa tctttggata acgatatgct ttcaacgttg
    29161 ctgtactttt tattagcatt ggtattgtta gtcaccatac atgaatacgg ccactttcaa
    29221 gtagcgcgat ggtgtggtgt gaaagtgtta aggttttcat ttggctttgg aaagattctt
    29281 gcgcgttttt atgataagaa aggaacagag tatgcctggt ctttgtttcc tttgggtggc
    29341 tatgtcaaaa tgcttgatga aacagaagga gaagtttctg aaaaagaaaa gcctttcgct
    29401 ttcaataatc aatctgttct tgtcaggata gctattgtag tagcagggcc tttattcaat
    29461 tttatttttg cgtttgtagc attgtggtta gttttggtta tcggcatgca ctctttggct
    29521 ccgatgattg agtcagtaaa accaaatagt attgctgcac gagctggcct agtccccaag
    29581 caggaaatcc ttgctcttaa tggagttaaa attaatagtt ggcgagattt tcaatatgaa
    29641 ataatgcctc ttatcggttc ccaggaaaca gtgagcttga ctgtaaagtc aatgataaat
    29701 ggtgaagaaa gcaccttgta tttgcctttg gataagtggc agttggatga taaaaagcct
    29761 gatcctatta aaagcctggg catcattcct tttataccga cgattccacc catagttggt
    29821 gaagtggttc ccgattctcc cgctgaaaaa gcaggcttaa aaatagggga tgaaattatt
    29881 agtgtcaatg gtcagcattt caatgattgg ttgtatttag tcagctatgt aagggaaagg
    29941 cccaattctc aaatcaattt ggatattaaa cgccaaggta aattgctgaa tataacggta
    30001 cacactggaa gtcaggataa taatggaaaa ttagaggggt taataggagt tagatctcag
    30061 aaagtagatt ggcctgctca ttggctacgg ctggagcaac agccaccaat ttcagcgctc
    30121 ggtacggctt ttaagcaaac agtacaacta acaggcacaa cttttatctt aatgggaaga
    30181 ttagtgactg gaaaattggg attaaacagt attagcggtc ccgtcggcat agctcagggg
    30241 gccggcgact ccggccgcgg aggattggta tcctatttat tttttctggc tttagtaagc
    30301 attagcctgg gagcgctaaa tttattacca atccctatgc tggatggcgg tcatcttttg
    30361 tattatgtct tggaaatcat aagaagaaag ccattatcgg acggagttaa atctgtcggg
    30421 atctattttg gattattact gttggttgct ttaatgtttg ttgctcttag taatgatata
    30481 tcaagattaa ccagctagga tttggcgaga gtaattctaa tttctgtatg attaatgaat
    30541 gaaatgatgt tttagcgaca aagaaacttg acagtggttt ctagttccta taaaagggtt
    30601 tcaatttttt gtacaattga atggttttta acttcataag tgcgcgtagt gctggacgta
    30661 aaataataat gaaaaaagtc agtaataaat taatattagg tgtttgttgt tctactcttt
    30721 tagcttggtc atcccaatct ttttcttctg atacctttat tgtcaaaggg attagagtta
    30781 acggattaca aagggtttcg acaggtacgg tattaaacta tatgcctgtg caagtgggtg
    30841 aggaaatcag ttccagctca acagctcaaa ttatccgcgc tctctatgag acaggatttt
    30901 tccagtccgt ttcgcttgaa cgccaaggga atgtgttagt ggtcaatgtg gtagagcgag
    30961 caactattgg ctctattacc gttgtaggaa ataaggaaat accctctgat aaaatgaagg
    31021 cttttcttaa agaaatgggg ctggtcaaag gcagagtatt tcaaagatct tccttggagc
    31081 gtttggagaa ggagctgaaa caggcctaca cagccagagg gaagtataat tctcgtattg
    31141 aaactaaagt aactcccctt actgaaaata gagtagccat tagtattact gtatcagaag
    31201 gtcgagtttc acggattaaa gaaataaaaa taattggtaa ccatgatttt aaagcaaatg
    31261 agttattgcc tgaattgacg ttaagtacaa gcaatctgtt tacttatttt actaaaaaag
    31321 atcaatattc caaagcagga atggatgctt ctttagaagc attacgttca ttttatttag
    31381 ataggggata tttgaaattt aatgttgtct cttcgcaagt tttgctatcg cctgataaaa
    31441 aagacgtcta catcaatatt catatagaag aaggacctca atatcatttc tcaggttatg
    31501 atgtggttgg aaaaactata ttacctaaag aaaaaattga ttcactgatc caggttaaga
    31561 aaggcgatat tttttctcgt aagaaggtta ctgaatccat ctctgcaata gggttagctt
    31621 taggggatgt aggatatggt tttcccgcga ttaatgctga acctagaata gatgaaaata
    31681 ataaaacagt atttattact tttgtagtgc agccgggccg tcatgtttat gtgagacgta
    31741 ttaattttca tggtaatacg aagacaggag attatgttct gcgtaatgtg attcgtcagg
    31801 atgaaggtgg gttactgtct ttacataaca tcaaggaatc tgagcgtcag ttacgaatgt
    31861 tgggctatct aaaaaatatt gatgtcaaaa cgacgcctgt tccaggaacc aataatcagg
    31921 tcgatttgga tgttaatgtg gaagaagctc cttcagcaga agcaagcgca tccatgggat
    31981 atggtacaaa cggttatcaa tttaatgcat ccgttaacca acgcaatttt atgggaagtg
    32041 gacgttccat gggggctgct ttcaatgcaa gtcaatgggg gcaagactac tcttttaact
    32101 attataatcc gttctatacc gatactgggg taggccgggg aggaagctta tattattcaa
    32161 ggattgatcc taaaaattta aatgtcagta catacagttc taatcgctat ggtggcgaca
    32221 tcagctataa cttcccgtta ggtgagaaaa gcagttttca attgggatat ggttatcaag
    32281 atatcaatat taagtcagtg ggttacgtac tccccataat caactttgtt gctttaaacg
    32341 gtaatcattt tcaggaaata aggttgacat ccggttggag tagaaacagc tatgatcaaa
    32401 tgccttatcc taaccaggga tttaatcaac aagctattgc tatggtcgcg ttaccagcaa
    32461 catcgcaatc tctatcttac tataagagct cttatcaggc gcatttatat tatcctctaa
    32521 cccgtggctg gattttttct gttctgggta atgtggggta tggaaatact tttgataatt
    32581 tcggattacc cttttttgaa aactactatg ccggtggtcc agttcaacca ggccaggttc
    32641 gaggatacga tagttattct ttaggcccgc aagataattt tgggaatgca atgggtgcga
    32701 atttcctggt gaatggaagt gtaggcctga tactaccata cccattgagt cgagataatg
    32761 taagaacgac tatttttgct gatgcgggta atgtatttgc ttccgggaca cctcctgctt
    32821 tacgtggaac tccggcaggt ccgatgcgtt attcagctgg tgtgtcatta gaatggcgct
    32881 caccttttgg tccattgtct tttagcttgg ctaaggcatt gaatccacag ccattggatc
    32941 agactcaact cttccaattt gctctttcct caggttttta gaggtattat ggattagagt
    33001 aagcggattt tagtatttta aaaactagtg ctgtacatat cgcaaccttt ttcgaattgt
    33061 gctagcattt caaactttaa attaggagat cattatgaaa cgtctgggtg cggtgttagt
    33121 cgcattattt ttgggtttgt tcggcacgac tgtgtttgct gattgggcaa agataggcgt
    33181 agttgattta caaaaaatca tgcaaacctc aaatcaaatg aaggaaatcc aacagaagtt
    33241 ggaaaaagaa tttaagcctc gtcgagacaa gcttgtcgca atggaagcaa gcttgaaaag
    33301 cgacatggaa aaatttaagc gtgatagtgc tataatgagc gcaagccaaa agaaagaatt
    33361 ggagaagaaa attgttgcgt cacaacaaca gtttgaacgt gatggacaac aatatcagca
    33421 agaattgagc acggctcaca atgaagctat ggaaggtttg tataataagg ttcgtactgc
    33481 cattaccaaa attgccaagg atgagaagta cgatattatt gttcagaaag atgcagcgcc
    33541 cttcagcagc gaatctcttg atgtaacaga caaagttatc aaagctatta attaattcgg
    33601 cggatgcgtt gctaacctta gaggaactgg caaactattt aaatggcgtt tggcatggca
    33661 atgccaatga cgccattttt agtctatcct cgttggctcg agcttcatct aaggatcttg
    33721 cgtattttga taatccctta ttgcagtctg tcttaaactc taccagagca ggtgctgttt
    33781 tattaaagcc cgagtacaaa gattggtgtc ctgtgaactg tattgtagtt cccaatccgt
    33841 cagaatcaat gtttaaagca gcaaagatcc tgcctcacct tggtaccaat gtttgcggaa
    33901 ttcatcctac agctcaaatc cacaaatctg cccaaatagg ccaacacgta tcagttggtt
    33961 ctaacagcgt gattggtgaa aatgtacaat tggatgatta cgtatctatt ggttctggta
    34021 cgaccattga atcttcagta ctgattggca gaggaagtca gctgggttct ggagcaataa
    34081 ttcattctgg tacagttttg ggccaatctg taattattga ttctgggtgt attgttggtg
    34141 ctgccccgtt taattgctac aaggaacatg gtgtctggca acaagcacca aactttggtg
    34201 gagttgtaat tggccaaaga actcaaatcg gtgcaaatac agtaattcac agaggctcaa
    34261 ttggcgatac ttatttaggt gatggagtct gtatagacag tttagtactc atcgcgcatg
    34321 atgtttatat agggaataac actgctattg caggttctgc tgcaattggt gctttagtgc
    34381 aaataggcag tgattgtatc ataggtggag caagttgcct tgcagcgaac attcgtttga
    34441 ctaatgatgt agtaataaca ggaatgtcta cagtgacaaa atccatcgct cgccccggga
    34501 tttattcttc gggaacgacg gttcacgatc atcgcaagtg gcgtcgtaac acagcgcggt
    34561 tcaggcattt ggatgactac attacaaagc tgataacaat agaaaaaaaa ttgaataata
    34621 acaactaatt atgttttatc aatttgaagt aacattattt ttgaaatttt taattgatta
    34681 gaatgagcta aaccaagata atgagccatt ttgtaatgca ataggcgatt aatgagaact
    34741 agggtatgaa cgaatcgata gatataaatc aaatttttac tttgttacca caccgctacc
    34801 catttattct ggttgataga gtgatagatt acaaagtaat ggaatatttg attgcaatca
    34861 aaaatgtgac tattaatgag aacttcttta ccgggcattt tccagggaat cctattatgc
    34921 ctggggtact aatgcttgag gctttggctc aagctagcgg cattttagcc aatttgtctc
    34981 gccaacctaa agaggggtat gaatttctgc attattttgc cggtattgac aatgctcggt
    35041 tcaagcaagt agtgattccc ggggatcaat tgcgattaga ggtgagactt gttgggcaaa
    35101 aaagagattt ttggcgtatg cacggagaag cttatatcgg tgataaatta gcttgttccg
    35161 cagacttgtt gagcgcaaca aaggaaatta aaaagtgata gatgaaagag caatgattca
    35221 cccatcggcg aagctggcaa gcggagtgtc aataggcccc ggaacagtaa taggcgctga
    35281 tgttgagatt ggtgaaaata cctggatagg gccgcatgtg gtaattgaag gacctactgt
    35341 aattggtaaa aacaataaga tctttcaatt tgcttccgta ggagatgagc cacaagatat
    35401 cacttacaag ggcgagccaa ctcgtcttga aatcggcgat aataatgtaa tcagagagta
    35461 ttgcatgatt agccgaggta ctgtcaaagg tggaggtgtc actcgcattg gcaatagcaa
    35521 ttatttaatg gcctattcac atattggcca tgattgtatg gtaggcaatc atattattat
    35581 ggtcaattat gcagccttat caggtcatgt cacaattaat gattacgcaa ttattggccc
    35641 ttatgctgca gttcaccaat tttgtcaggt gggagcctac gcattcatag cccgagctac
    35701 ctatgtcact aaagacgtat tgccctatgt tatgattgca ggtcatacca cttctgcttg
    35761 tggaatcaac actgttggtt taagaaggag aggtttttct tctgctgcga ttgattgttt
    35821 gcgacgcgca tataaaatta tctttcgcaa agggttaacc gttcaacagg cagtttcaga
    35881 gttagagttg attcaaaatg agtgtccaga aattattcct atgattgatg ctttgaatca
    35941 atccacaaga ggcattgtga ggtaagcaat ggttgttgca ccctatttgg ctgtagctat
    36001 cggaggttct ttaggcgcaa tgtctcgtta tttagttaca ataatggcgc aaaatgcctg
    36061 ggggattaaa tttccttatg gtactctttt agtgaacaca ctaggttctt ttctagctgg
    36121 tttttttctt attgtattag taggtcgttt tagcgccgag gagtcgtttc gtttattcct
    36181 ttttacggga tttttaggag cttttactac cttttcgagt ttcgctgcag aatctttatt
    36241 tatgtttgag caaggatatt ggtttaaatt aattaccaat atactggtta ataatgttgg
    36301 ctcattgtcc atggtcttca ttggaacatt agttgctaaa tatgtattat taggacatca
    36361 gggctccaat taataatttt tagagtgttg gcttatgtta gataatcaat tacttagaga
    36421 gaatcctcaa tatgtagcaa cccaattgct gaagcgagga tttcaatttg atgcggtcac
    36481 tttcagtcag ttggaagaaa agcgtaaggc tttgcaagta agcacgcaat cattacagaa
    36541 cgaacgaaat ttgcgttcga aagccattgg tgaagcaaaa tctcgaggtg aaaacataga
    36601 acctatgcgc gaagaagtga ataaacttgg cgctaaactg gagcaacaaa agactgacct
    36661 ggaagaggtt cttaagcaaa ttgaagcaat tactctgtca ttacctaata ttcctcatga
    36721 atcagtacct gttggtaaag atgaattgga taatcaggaa atcagaaaat ggggcgacgt
    36781 acctgctttt tctttccctg taaagtcaca tgatgaattg ggtgaagcac tagggcaaat
    36841 ggattttgcg cttgcagcta aaattacggg cagtcgtttt gtcgtcatga aagggcatct
    36901 tgcccgatta catcgggctt taattcaatt catgctggat atacatattc agcaacatgg
    36961 ctaccaggaa atatatgtac cttatattgt taatgcggat agcttactag ggactggaca
    37021 gttgccaaag tttgaggctg atttatttaa attaacagga gataatgggt actacttgac
    37081 gtctacttca gaaattccag tgaccaatac agtaagggaa atgatattgt ctgctgagca
    37141 attacctatt cgttatgttt gccattcccc atgttttcgt agtgaggcgg gttcttacgg
    37201 taaagacaca aagggaatga ttagacagca tcaatttgag aaagttgaac tggtctggat
    37261 taccaaaccg gaagattctt ataatgctct agagcaactt actcagcatg ctgaagttat
    37321 tttacagcgt ttgaacttgc cttaccgagt ggtcgctttg tgtaccgggg atataggcgc
    37381 cggttcagcc aaaacctatg atcttgaggt ttggttgcct agccagaata cttatcgaga
    37441 aatatcctct tgttccaata tggaggcatt tcaggcacgc cgaatgaaag cgcgtttccg
    37501 caatccggag actaatgaaa ttcaattagt ccacactcta aatggatcag gtttagctgt
    37561 tggaagaact ttagttgcca ttatggagaa ttatcaagat gagcatggaa acattcatat
    37621 accggatgct ttgaagcctt atttaggtgg tattgatatc atctctgtga aataggtttg
    37681 ctcataatta tggatatttt aaaagttagc cttcaacagg aaagagcgat agataggcta
    37741 tattgagcca aatcaattgc tcttaagtat ttttaagaaa aaaatccggt gactctaaac
    37801 taattcaaat gaaacatttt gatttttcct taagaatctg gccaaaaacc atagatatca
    37861 attattctga atcttaagtg tcatatttta gtttatgttt cgtgaaacat tctggagtca
    37921 agaatccaat gaatgcggta attattcgtg agataacatc tgaagataaa gagatgtttc
    37981 tttgtgctat gcaaagtagt caagcattac atcatccttg ggttaaggca ccgataatac
    38041 ctgaagagtt tgatgagtat ttttctcgct atcaaaaacc aaaccaaaaa agctatctgc
    38101 ttctttctga caataactta gcgggtgtct ttaatatcag tgaaatcgta cgtggttact
    38161 ttcagagtgc gtatttgggc ttttatgctg ttgcagctta tgcgggtaaa ggatatatga
    38221 gtactggttt gaaattaata ttaaccaaag tttttaagga catggggttg catcgcttag
    38281 aagcgaatat acaacctgag aatacccgtt caatctggct tgtgaagaaa aatggttttc
    38341 gctatgaagg attttcacca cgatatttga gagtgaatga cgtgtggcaa ggtcatgagc
    38401 attgggccat gacttatgaa gattttatca aggataataa tgaggtactt gaaaaagatc
    38461 atatcgatat tgttgcatac aatacagagt ggccgcttct agccaaggca gaaatggtga
    38521 agttacgcgc ctcatttccc gcaaatagtg ttattgatat ccagcatgta ggtagtacag
    38581 caatacctgg catggcagca aaacccatta ttgatattca gattgcagtg aggtcattag
    38641 aggaaatgaa gattattgct gtgccgatat tacagaaatt agcttatgaa tattgggaag
    38701 ataatccaga tcctgagcga atgttttttg taaaaggcat gccaccctat ggtaatggac
    38761 gaacgcacca tgttcatatc gtcgaagcat catccagaca ttggaaagga aaaacatttt
    38821 ttagagacta tttactctct catccagaag cagctgaaaa atatcagcaa ctaaaaatca
    38881 aactggctca ggagcgcaaa tatgataggg agcaatacac tgatgcaaaa agtgaattta
    38941 tcaaccaaat tttaaaatta gctcaaaatt aataaattat catgggcaga caaggataaa
    39001 tgggtgtaac gaacttggat ttttgggctc agattagatc taaatggtgc atcaacgtat
    39061 ttagaagtcc taatcttaat attgtgatct acaaacattg tgatctataa ttgtactaaa
    39121 tcactctagt tgagaaacgc aatggcagat caacccccaa aatccaaatg gcatgaaatt
    39181 aaacgtctat cagaaggtga taataaagta gataacaatc aagcaccagt ggcaacggcg
    39241 gatagaatta aaaagctcta tctggataca caggtaggca gtccaattga tactgttgaa
    39301 gaagcggatg cccagcgaaa taatttagga cctaaagata ctaaaataga aaattcatcc
    39361 catgagaatg atgagccaaa ccctgatata ccaaccccgg gatgatgtca gaaattaaat
    39421 acattgttta aataagctgt tttttgctgt ttaactatac cattctggtt cggcaaacgc
    39481 atgcgttggt tttgtgttga cacccataat aaatacttgt aaattttcct acaagaaaat
    39541 ccttagtgtt cagtattctt ttagtcatcc tgattgaaca cgcgattagg tttttggtga
    39601 tggcttcttt attttttatt aaagaggtca taaccgtgtc agataaacca gatttcccat
    39661 atatattaag cctgcaagca gacggtaatt tatttcctag cattttacaa tcaaatttag
    39721 ccatgacatt ggtttggagt tgttatggac aatttattga atgaaaaaaa attaacaatt
    39781 tttacatcta atgaaatccg agataatttg aaccagattc tgaaagacta tgaacaaaat
    39841 ggcattattg gacttaatgg atatctcaat caggcaaacc gaataaactt gcttaatgaa
    39901 cttgaggtaa tgcaaaagga tgctgagcag gatattgcca ggtattggaa taataaaatc
    39961 attcattttt attccaaaga tcctttgaaa cctgagtcag tggatagaga atatgtcaca
    40021 gaaccttatt ttcaagcttc aagtaataag gcacatgtat tttatgaagt gattgataat
    40081 atgctggtag tcaatcgaat tggccatggt atgcatctca tcaataggta tccgatgatg
    40141 cagcgagcag tttatgatag tcctgtactt ctggatttgc taaaagggat tggatttaaa
    40201 aagccaatct gccatctcag tgtttatatt cccaaatatc ctaatggaat aggaagtgag
    40261 gtaagacctc atcaggagtc aacatttgcc tttactgaac cacaaagtgt cgttgttctg
    40321 tgggtcgccc tggaagacgc gttaattgag aatgcctgca tgtatggggt tttaggaagt
    40381 aatcattggc cattgaaatg ggtatcaaaa gtggaccgag agacaaaaac gcgtcacttt
    40441 gagcaagttc atcagttaca tattcctgat tttataacag agcgtgaatt ttatactgcc
    40501 ttggaagtga aagccggaga tgctttgtta tttcacggta attttgtgca ctgcagccca
    40561 atgaatacgt ctaaaaactc aagaaaagca ttatcgtttc agtttattga aacgcatgaa
    40621 gtcgattatc cggaaactaa ttggttatac cctcctaaca aagtgtattt atattaaaaa
    40681 ttaaatttga taagcgattg aggaatcact acatgtctat gtatgataag cgccagtttt
    40741 ttaaaaccta tttttctagt gcaagcgaga agtcaatcct ggtaaagctg ttggataaac
    40801 gatttcatcc tccctctcat actgcgaaca tattggattt gggttgccat gatggcgcat
    40861 tgatgaaaaa tatagtgaat gcttacgcaa agcgtttacc ggaaaagtta gttattaccg
    40921 gagtggatcc tagtttgcaa gcaattaaag aatatgcaaa agcagatttt ccaattccag
    40981 tccaaacgaa tacttttgct ggaactgcag aagattattt tgatgtgtgc acagaccatt
    41041 ttgattggat ttttgcatcg cagtgtttgt attggtcccc tgatttgcca aaaataatta
    41101 agcagattca tgaaacagga gactctgctt taattgttat cagaggatgt catggaattt
    41161 atgaaattca atcgcaattc agacattata ttgggaatca tttagagaaa ttttatactg
    41221 ataaagaagt tgagatggcc ttaaggaccc aaaaaatttt atttcagaaa gaatctctaa
    41281 gcacttctat ccggttgcct gataaagaca gcattgaatt gaaatggctc attttgtttt
    41341 ttttgcaaat ggaagaggga tcgttgggtg ataaaatatt ttgcgaagtg cgggattgga
    41401 ttacctctcg aacgtcagga agcattcatc atgaagttca ttttttttgg ttagggaatg
    41461 ccatttttac aggggattac aatatgaatt tcccagtcaa atcgaaaaaa aattttatgg
    41521 gataaaggca atgttttcaa tgaaattgat ttcatcatca gtcagcctga actgatggat
    41581 agcaagatct tctttcatgt gatccaaatt tgtcgtgcca gttaaaggca acattcctat
    41641 ctgttgtgaa aatctaaaaa tgatttgctc aatcgatttc tcgtactttt tgctgagatt
    41701 ctcaagtttc ggatgcctta actcatattg attggccgtt aacagagaaa acccctgata
    41761 aataatttca tgagcctggc aatagttacg aatttgttta tcccataata gcctggcaaa
    41821 gcatctattt tgaacaaact tgggttttac cgaagcaaat gaaatgagtt cgactagctg
    41881 ttcataactc acattggata tgcccaggtg ttttactttc tcatctttat gcagggattc
    41941 cattttgttc cagacttctt tatcttcatc agaaaaatga tattgataat aagggccatg
    42001 caaaatatag gaatttaaat aatcagtatt taaatgttca aggctgcttg caaaagattg
    42061 ctggacttga atagtcaggc ttgctgtatc atcgtaaggt ttgcgatgat cctggccatg
    42121 agcataggtg aatttggatt gcaaaaaaat atcatcacgc aagatacctg attttttata
    42181 gtattgttgc aaggcatatc caacaccttc ttcataatag tgctttcttt ggtttgcagt
    42241 atctataccc tgaaatccgg tagttaatgc aagctcgacc agttcgcttg tacgcttttc
    42301 tttccaggcg gttccatata taaaagttgg aaagccaagg tccatcgttc aatctccttc
    42361 tcgatttatt atctgcaaac agactttatt ttgactcaaa aaacagagca aaccatagct
    42421 gactgtgtgt cttgcatagg gtttttcctg agatttcttg tcttactatt tgttcaggac
    42481 ttacgaaaaa ccccaaggtc aaggcaaaaa atctttttaa tgagggagtt tagataaact
    42541 aaatgaccga attaaaaaca ttttttaaca tagagattgg ggtttttcgt aagtcctgtt
    42601 gttttcattt tcaatagtta caaatcctta cctaaaagac aacaggcggg attaattcga
    42661 aagtttattt gaagaacgaa ctaattttat ggaattatac aaggaaatca gtccttaatc
    42721 acaaagagct tggtattttt taatgagcta cagtcgcaag cgactgtagc aggcaggtaa
    42781 gaattagttg aattgataag cggttgtatt ctcatgctca gtctcagtca cttctggttt
    42841 gaggcaagca gaaacccagg tggtaccaag acgtgtcata aaggcaattc cttcgaccag
    42901 agcgttaatt ggtgtcatca cagccaaaga gacagccaaa ccgaaatgta cttgtaagtt
    42961 atggaaattc tcttttgcat gtaaaggctt acagatagcc aaattgacaa tacattctat
    43021 cgcagactca actccataca atgcttcgac taatgaatga aatccgcttt tagccaagga
    43081 ggtcattggg gatgccattt gttctaaaca atcccaggca ttgttataag aagaaaagaa
    43141 cctggcttta ttgaagtaat tggaagaagg caattcatta accatttgat atccacttac
    43201 tctgtttgcc cactttgcca taagcccggt ttctaataaa tcatggcgag gttcttgacc
    43261 aacaaaccat gatgatataa ctcttgtaag aatttcaacg gcatgtgtca gagcatgaat
    43321 tggagccata agcagtaaac aggtagagag agttaaatgg caacccacat ctttgatcgc
    43381 gtccaatgcc aagcctggtt ttaaaattaa taaattacct acagttcgca aggtggccca
    43441 tactgccctt gaggcatagt agcaagcata tagcccattg ataatagggg taattcctac
    43501 taatgccaaa ccagcccaaa attcatttgt attggcataa tgattaataa atcgcttggc
    43561 tgaaacatat tctctatctg gcaataatcc attatgaatg agcgattcaa tttctgttct
    43621 aaggtctgtc attatggtct ctctctatgt taattaaaca agcggggaga gtataaccaa
    43681 aaaaaacata aatgactaat attggcccat gttgtatttg atgtttgcat ctgttttcca
    43741 atgtcttttg aacacagacc aaaagaccca gtaaaaagca ttggcaaatg ggccacaagg
    43801 gcctgagtta attgcaggtt caccaaaatt gtccccagca atggtgacgt atccatagag
    43861 tttatttatt tctttagata agctatttag ggtattaaga acattatttt cggcattcat
    43921 aaacaattgt ctcatcttgg gcatctggag ttgcagcact atcaaatgct ttaatcaaac
    43981 ggacattttt aaaaccacaa cttttaagaa ttcctgttaa ctcctgcggg tcgtaaattc
    44041 ttactctcaa ttcttcaacc tccgtatgaa ttatactatt gttgtgaacg agttcatatt
    44101 taccgataga atgacaaacc tgatccttca tggtcgctaa ttgactgagc ataatcatct
    44161 ggccattggg cttatgccaa actgagcctc tccagacatc gagttgtggc actgctttga
    44221 gtgtttcgcc ctcaaacaat aaaacaccct catcgcttag gtgattataa aatgtttgaa
    44281 gtgctagtct tactgttgca ggatcgataa gcaagcaaaa ggaaccgctt ggtataaaaa
    44341 tcaaattgta tttttctggc ctcgttaaat tttcaacaaa acctctccat actgttggtt
    44401 tgagattttt agctttggct ttaatattca gggcttcaag catatattcg cttgcatcaa
    44461 agccatgtat ttcgaatcct tcttcaagta atggaagtaa aaaacggccg gtaccacaca
    44521 ttggctctaa catagggtct ttcacattca ttacataact gcgataaaat gcataagcgt
    44581 cttcaggggg attgggtttg cttaagtcat aaacctcagt gcacaagcta agataagtat
    44641 ccaggtttct taattttgtc atcttgttca ccaattaaaa aatttttgat tatttgaatt
    44701 tgcacttcgg tacttaggag accgtcaatg actatagtgg cattttgttt cagttcatca
    44761 tcaaaaaaca aaggtcttga atggtttaaa taaaaactga tttcttcaag aagattttct
    44821 ttagtttgta atttatcttt aaaatccctc aaaatgcgac ggcacaatga aatatcaagt
    44881 ggcacctcaa tatgaacaca ggtatcaata tattcacctg tttgtacgtg aagtttccct
    44941 aatggggcat caaaaattat gtattccgcg gggttgagca aagtgcgtaa gactggatga
    45001 gtaatttctc tttttgcttt aagctctgcc aaaacctttg caaggttttc cctttgaaat
    45061 tcactataat tacaaccctt atgataccac tcgatataat cttcgggttc taaagaaatg
    45121 tcatcaaagt catcccatga aataagagtt gcttttaaat ccagggacaa tgcttttgcc
    45181 aaagtagatt ttccagcgcc ggttataccg ctaatgccaa taatatgagg agtcataata
    45241 atatctccat tacagtgact ggaagtcctt taaatttatc catgccgatt atggcccaac
    45301 caaggcggga gtaataatta ggaatggttg ggtcaaaggc aaaaagatgc agtttattaa
    45361 aacctaaatc tctggcttta ttttttgtgg cttcaattag cctgtttcca ataccttgtc
    45421 tctgataggc agggtcaacc acaagcgagc caagccatgg gtttatttct ggcctgatac
    45481 catcattttc acgcaaagag cacatcccaa cgggtttccc tgtatcaagt gcgataaacg
    45541 taatgggtag tttgtcagca tttaggtgtt cactgaaccg ttgaataact tgttcaaccg
    45601 agatctcggg aacccagatt ttcccaagta cttcatgcca aattgtcgca caccttgcta
    45661 tgctttgtgg atgatttttg agaaggtcga tggtgatcat gctttacctg cattgacgag
    45721 ttcaggtatt aaatttttaa ggcgtagaat agtagttggt aacatttgtt tgagcacaag
    45781 gatcttggca taaaaagtaa aagacttgtt gaggtcttta attgctaaag taatgtgatt
    45841 tattccggta atcatttgat taaaccaatg cgtttaatca ataaatgatg aagctttccc
    45901 gcgccagaga cgtcacctga catcaaaaaa tcagcaatat actcaaaacc tgcttttata
    45961 taaacatgct tggctctggg attgtcactt gcgggatcaa ttaaaaacgt atcggctttc
    46021 ggatcgactt cttttataaa aaaatcaata aattctatca aggttctggc tccataacct
    46081 atgccaaaat agtttttatc accaatcatg taatcgatac cataagaatg tccagtcttg
    46141 gaaaggttaa tcaatttaat ttcgtcgatc tcatcttcgc ttgtttcctg gattgtcatc
    46201 agcattgcga aaggatgccc attaccgctt gctatccaat aaacatattt accgtccgca
    46261 taggatgagg gttctgtgcg gccattgata aagtttaaga tgtcatcttt atgagcttgt
    46321 gtgttgtccc agaactcctg aacaaagggt tctttaagcc agctaaagat aagctcgata
    46381 tgacttgcat tggctttttc aaaatttatg ttcattatgt gaaaaattct aattttttga
    46441 ttttatatgg tcggagtgac aggaattgaa cctgcgacc
//
LOCUS       DBNCWK010000012        46462 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.012, whole genome shotgun sequence.
ACCESSION   DBNCWK010000012 DBNCWK010000000
VERSION     DBNCWK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 46462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46462
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.012"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            59..718
                     /locus_tag="AC06IO_000561"
     CDS             59..718
                     /locus_tag="AC06IO_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIO9873776.1"
                     /translation="MEQWHQFTLLFPLIILVFISLISPGPDFAITVKNSLAYSRRSGL
                     LTALGIALGSLVHISYTLLGLGLFITKTPVILHIIQYLGAGYLFYIGLKNLGAKPNAD
                     QIGSYTTYNDLKPSEAFFNGFITNLLNPKAMLFFISLFSVLMPREISISNKLFLAIIL
                     FLQTLLWFSFVAFSLSGKNTRTKFQRVSYWIERITGVILIGLALKLLLSQLGSVDISP
                     A"
     gene            complement(842..1390)
                     /locus_tag="AC06IO_000562"
     CDS             complement(842..1390)
                     /locus_tag="AC06IO_000562"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9873777.1"
                     /translation="MKYLTTPRILLRAWDEEDTIPFYRMSQDPRVMKYFPGLWSMDMV
                     KDFIIRMNEQLSQKNFTLWAAEVKDCKQFIGFIGLNAPTWNAHFTPCVEIGWRLATEF
                     WGQGYATEGAKVALEYAFLEMHIPEIVSFTVPDNSRSRSVMDRLGMIRDIAGDFLHPK
                     LASDHRLAKHVLYRIQNPLIKV"
     gene            complement(1387..2175)
                     /locus_tag="AC06IO_000563"
     CDS             complement(1387..2175)
                     /locus_tag="AC06IO_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIO9873778.1"
                     /translation="MKSLPIYQVDAFASNLFKGNPAAVCPLDKWLSDETMQAIAAENN
                     LSETAFFVPETDGYRIRWFTPNEEVALCGHATLASSYVLFNQLGFSGEEIIFNSLSGK
                     LWVKNTGQGLVMDFPVLPYEEINFSAELQHLNLKRPFKTLRSQFDLMFVYQNAEEVVE
                     AKPDLNAVAHLDYRGLILTAPGISTDVYSRCFYPGCNVPEDPVTGSAHCVIAPYWCEQ
                     LRKNHITALQGGLRQGDLYCEVKENRVLLYGNCHLYLEGRIYLP"
     gene            2253..2672
                     /locus_tag="AC06IO_000564"
     CDS             2253..2672
                     /locus_tag="AC06IO_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIO9873779.1"
                     /translation="MYLSSLLKKTNRLLIKRANELIKPYAMSHAYTYILMELYIEDGL
                     TQSELVQLIEVEQPTLVRTLDRMERDGFITRKPSSMDRRVVHIFLTEKARLLQEKIDE
                     CSRILNNEILAGFSEDEKTKLQALTHRVLDNLRNPIE"
     gene            complement(2702..3157)
                     /locus_tag="AC06IO_000565"
     CDS             complement(2702..3157)
                     /locus_tag="AC06IO_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIO9873780.1"
                     /translation="MPVKKLKQFLDSHKIKYLSIAHSPAYTAQEIAASAHVSGKQLAK
                     TVIIKMDGRLAMVVLPASDHITFMKLKEAIGTSDLELATESEFEGKFAECDVGAMPPF
                     GNLYGLPVLVSTKLSGQDNILFNAGSHSELMQLSFGDFEKLVKPTLVTL"
     gene            complement(3317..3889)
                     /locus_tag="AC06IO_000566"
     CDS             complement(3317..3889)
                     /locus_tag="AC06IO_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIO9873781.1"
                     /translation="MLLPIQVTFRNMDPSKAVESRAIKLAKKLDCLYDKIMGCRVVIE
                     SPHRHHNKGKLYHVRIDLTVPDAELVVSRESSNKHAHEDVYVSLRDAFNAIERQLKNY
                     ANQRRGHVKRHEDSIRGGRVLEVCPIADYGYIETIDGRLIRFTGQSVVDYDINKLEVG
                     DRVRFVETVKNTQEAAASTVYVEGKRHIMN"
     gene            complement(4401..4634)
                     /locus_tag="AC06IO_000567"
     CDS             complement(4401..4634)
                     /locus_tag="AC06IO_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIO9873782.1"
                     /translation="MSQRETGHVKWFNEKKGFGFIVNQKGEDIFVHYKDIQGVGFKTL
                     HENDPVTFELDRGPKGLKAQNVVVASESVHTEE"
     gene            4883..5518
                     /gene="pyrE"
                     /locus_tag="AC06IO_000568"
     CDS             4883..5518
                     /gene="pyrE"
                     /locus_tag="AC06IO_000568"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIO9873783.1"
                     /translation="MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGN
                     SIRQLGQFYAKTLLEQEISFEHLFGPAYKGIPLATATAVALAELGKDITVTFNRKEVK
                     THGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
                     TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYHELYGAY"
     gene            complement(5755..5856)
                     /gene="cydX"
                     /locus_tag="AC06IO_000569"
     CDS             complement(5755..5856)
                     /gene="cydX"
                     /locus_tag="AC06IO_000569"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HIO9873784.1"
                     /translation="MWYFSWILGTGLACVFAVLNAVWLELREEDVKQ"
     gene            complement(5869..7005)
                     /gene="cydB"
                     /locus_tag="AC06IO_000570"
     CDS             complement(5869..7005)
                     /gene="cydB"
                     /locus_tag="AC06IO_000570"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIO9873785.1"
                     /translation="MPLDYETLRIIWWLLIGILLIGFAIMDGFDLGVAMWLPWLGRTD
                     IERRILINSIGPTWEGNQVWFILGGGAIFAAWPTLYALSFSGFYMAMLVVLLALILRP
                     VGFKYRSKLDNPTWRAFWDWALFIGGFVPALIFGVAVGNVIQGVPFYFDESLRVFYTG
                     SFFELLNPFALGCGLLSVFMLAMHGAFFIQVKTVGALQNKAKDRARWSAILVILLFIG
                     LGIWAYYGIDGYVLKGMVPHDGPSNPSYKDVSIQAGAWFNNYKEYPVSLLVPVLCIVS
                     AFLAIIFANKSVFAFVLSGLSVGTLIATVGVSMFPFILPSSSNPKYSLIVWDSSSSQV
                     TLFIMLVATVLLLPIVLAYTAWVYRVLRGKVTESSIKAEQDSAY"
     gene            complement(7021..8553)
                     /locus_tag="AC06IO_000571"
     CDS             complement(7021..8553)
                     /locus_tag="AC06IO_000571"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIO9873786.1"
                     /translation="MIPAAEVVDLSRLQFALTALYHFLFVPLTLGLSVMLAIMETVYV
                     MTGRDLWKKMVKYWGVLFGINFVLGVATGLTMEFQFGTNWAYYSHYVGDVFGAPLAIE
                     GLMAFFLEATFVGLFFFGWNRLSKLQHLTSTWLLALGTNLSALWILIANGWMQHPVGA
                     YFDYQTMRMEVSSFYEMLFNPVAQAKFVHTISAGYVTGAMFVLSISAYFLLRGRNKAF
                     AKRSMTVAVSFGLASALSVVVLGDESGYVANENQKMKLATMEAMWHTEKAPANLTVFG
                     IPDEKTLTTKYAIDIPYALGIIATRSFNTPLQGIIELIEEGKLKIKDGMIAYDALKVL
                     QKDPKNEQAKAVFTKHSENLGYALLLKKYTENVTDATEEQINQAAHDLKPKVLPMFFS
                     FRIMVACGIYFILLFATGFYLSVRHRLEKTTWYHRIAFYSLPLPWVAAELGWIVAEYG
                     RQPWVVEGILPTFMGASSLTSGQVMTSIAGFVLFYTVLAIVEIYLMVKYIRLGPDGMN
                     HH"
     gene            complement(8558..8728)
                     /gene="cydP"
                     /locus_tag="AC06IO_000572"
     CDS             complement(8558..8728)
                     /gene="cydP"
                     /locus_tag="AC06IO_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase putative small subunit CydP"
                     /protein_id="HIO9873787.1"
                     /translation="MKPLTRDIVFTLSFKMILLVLLWYFCVRGMHPELSSNKEWLLGK
                     EEQTVLTPNNKR"
     gene            8929..9225
                     /locus_tag="AC06IO_000573"
     CDS             8929..9225
                     /locus_tag="AC06IO_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIO9873788.1"
                     /translation="MKIHGSFKKSALPNLMQAISLLKNEDEAMQFFTDLCTPAELESM
                     ADRWQVVPLLRQGIPYRTIHEETGVSVTTITRVARCLSFGTGGYRLIAERLEKL"
     gene            9222..10103
                     /gene="hisG"
                     /locus_tag="AC06IO_000574"
     CDS             9222..10103
                     /gene="hisG"
                     /locus_tag="AC06IO_000574"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIO9873789.1"
                     /translation="MKNRLRLALQKKGRLSDESLNLLQRCGLKFRIKPNALLTHVDNF
                     PIDLLFVRDDDIPTLVFDSLCDGGIVGENVLLEASFNRPDKAYRKILALGSCNCRLSI
                     AIPEAFDYQGAGSLDGKRIATSYPNLLNNYLAERKISAEILTLSGSVEVAPRMGMADV
                     ICDLVSTGQTLEDNKLKEVDTVLNSQAVFIQTQRQLDPDIQDLFAMLARRIQAVQQAQ
                     ERKYIVFHAPKSALERIAQKLPGAESPTILPLPGNNEKVAVHVVSSEGVFWNTLETIQ
                     YLGASSILVLPIEKMLE"
     gene            10109..11404
                     /gene="hisD"
                     /locus_tag="AC06IO_000575"
     CDS             10109..11404
                     /gene="hisD"
                     /locus_tag="AC06IO_000575"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIO9873790.1"
                     /translation="MLTIKNWQLLSENDKKLCLSRPRQSSAIKENVLEIINQVQLSGD
                     KALYDLTKQFDRFNLQYLQVPQEKIEQANIPQNALNAITQAIGTISSYHQSLLPENTE
                     ISTASGITIRNVYRPIQKVGLYVPGGNKTPLVSSLLMQAIPAKVAGCPIKVLCTPPDA
                     EGEINEHILVAARLCGIDTIYAIGGAQAIAAMAYGTESVIKVDKIFGPGNSYVTQAKT
                     LVAIDADGAAIDMPAGPSEVMILADTEANPEFIAADLLAQAEHGPDSQVILICDECEL
                     ANQVNQQLEIQMSYLSRIEFIKRSLANSRIIICSNQSEQLDIINSYAPEHLIINRKNP
                     EPWVEKIVASGTVFLGSWAAETMGDYVTGSNHVLPTNGFARNHSGLSTLDFMTRFTVQ
                     AINQEAIRNLGPAAMTLAELEGLDAHANAVQIRLNTLGD"
     gene            11405..12499
                     /gene="hisC"
                     /locus_tag="AC06IO_000576"
     CDS             11405..12499
                     /gene="hisC"
                     /locus_tag="AC06IO_000576"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIO9873791.1"
                     /translation="MSILNLVRADLLNSQNYVPGGESARYRSHANELPWSPVTMGEHN
                     LNYYPNIGLQIELQKQLAKRYQINSDQIILTRGSDDGIDLTTRLFLTAGKDAFMQFPP
                     TFPMYAFYVRLQQAELIECPLDRRTNFRLTLDQIENSWKPNCKVIMFCSPNNPTGNLV
                     DLNLIAKTCELYTNQSIIVVDEAYIEFANAPSATSLIGEFENLIVLRTLSKAFGLAGL
                     RLGCIIAQSPIIQVFNKIIAPYSIATPSMDLAKRALNNSDWFTKTIEQIKSSRAWVIK
                     KFADNPIIEKTYPTETNFILIQTRFSKQLATWLARYGIAVRDFPSSSLLHDHLRITVG
                     HDEQNQLLIDALSSFNADVAGLNYEKDFIY"
     gene            12477..13535
                     /gene="hisB"
                     /locus_tag="AC06IO_000577"
     CDS             12477..13535
                     /gene="hisB"
                     /locus_tag="AC06IO_000577"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HIO9873792.1"
                     /translation="MKKILFIDRDGTLVEEPFDFQVDSLDKIKLTSGVIPALLQLQKA
                     GFTFIMVSNQNGIGTVAFPEEDFAVCHEFILDLFSSQGILFDEIFICPHTPEDNCICR
                     KPKTGLLESYLKETAFAKQYSWVIGDRDTDKEFADNLGVNFLPISKTHTWEMVASAII
                     NDARKASVQRKTKETAIDLSVQLDSDQTSVIDTPIPFFTHMLEQVAKHGGFDLRLQAS
                     GDLEVDEHHLIEDTAIALGEAIRTALGDKWGINRYGYTLPMDESLATIAIDISGRSFC
                     DFKGQFTREFIGGMATEMIPHFFQSLSSALGATIHIEVTGINHHHMIEACFKVLGRAL
                     RQACSRTNNYLPSTKGVL"
     gene            13532..14131
                     /gene="hisH"
                     /locus_tag="AC06IO_000578"
     CDS             13532..14131
                     /gene="hisH"
                     /locus_tag="AC06IO_000578"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIO9873793.1"
                     /translation="MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAQEIQKASHVILP
                     GVGTARSGMTALQQNGLIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGVAE
                     LLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCRYHE
                     EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESKC"
     gene            14125..14844
                     /locus_tag="AC06IO_000579"
     CDS             14125..14844
                     /locus_tag="AC06IO_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HIO9873794.1"
                     /translation="MLVIPAIDLQSGRCVRLKQGRFDQVTQFSVFPIERALHFAKLGA
                     KRLHVVDLDGARSGKMQQLELICSMQKTGIAIQAGGGIRSIEQALECSNAGISQLVIG
                     SLAITNPDLTIQIIEKIKPENIVLALDVRVDTKVPLLAINGWQNNSTSSLWEVVSYYE
                     NHGIKHILCTDIACDGMMNGPNFDLYQQAVEYFPQIAWQASGGIRHMQDITTLGSLGI
                     SAVILGLMLYQDNVNFEELLC"
     gene            14838..15605
                     /gene="hisF"
                     /locus_tag="AC06IO_000580"
     CDS             14838..15605
                     /gene="hisF"
                     /locus_tag="AC06IO_000580"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIO9873795.1"
                     /translation="MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSASGA
                     DELVFYDITASSDDRSVCPVWVNKVAAAINIPFTVAGGIRTLYQARSILNAGADKISI
                     NSPALENPSLINELSKEFGKQCVVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREW
                     IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVF
                     KKSRVSGALAASVFHDKILAINDIKLALQTENIEVRL"
     gene            15602..16225
                     /gene="hisIE"
                     /locus_tag="AC06IO_000581"
     CDS             15602..16225
                     /gene="hisIE"
                     /locus_tag="AC06IO_000581"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIO9873796.1"
                     /translation="MSDLDINSLDWQKMNGLLPAIIQNAENGKVLMLGYMNQEALIAT
                     LTTGQLTLYSRSRKRLWRKGESSGNSMALQHISADCDNDSLLIQVIPKGPACHLGYST
                     CYQPHNNYTLGFLDGLIELINERAEEKNPNSYTTQLLDSGVNRCAQKLGEEAVETVIA
                     AVSNNKEELINETSDLIYHLLVLLKACELSFYDVLLCLQARDRSVHT"
     gene            complement(16314..17039)
                     /locus_tag="AC06IO_000582"
     CDS             complement(16314..17039)
                     /locus_tag="AC06IO_000582"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIO9873797.1"
                     /translation="MKNALKREDAANKKLGVLIYENVQPMDVIGPWEVFATWKTILDA
                     PIDMFLVAETSNEVCCASQISLKPHCEFNNSPQFDYLLVPGGVGRREQVNNPRLISFL
                     KKQAQNSDYLLSVCTGAFLLHAAGLLTHHEATTYWRAIPELKSFEDVQLVEKRIVKSG
                     KVWTTGGISSGIDLAFEFISTIAGNDVAGKVQLLFEYFPECKLYSQSAMVSELPSYYA
                     TKKDEAVLEMNLPDYIKEYFKKS"
     gene            complement(17179..18327)
                     /locus_tag="AC06IO_000583"
     CDS             complement(17179..18327)
                     /locus_tag="AC06IO_000583"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIO9873798.1"
                     /translation="MSLALKIKSIILSFFLFLMATVACATQVGLRDKIGQMLILGFQG
                     KVVDEKSPIAQAIEKNNIGGVILFDFNQQSQTFDKNIESPEQVNRLNNQLQTLTQKAN
                     RKYHRDNLPLLISVDYEGGNVNRLHPRYGFPEMPSAKDIGSMSLERADASAELMANTL
                     KSTGFNLNFFPELDVNINPDNPIIGKKDRSFSSIPEIVTQYAQLYTEQFIKHQIHCSY
                     KHFPGHGSSLSDSHLGFVDISDTWSEQELIPFLQLLSQPNHCDMVMIAHIVNRKLDMT
                     GLPASLSYQIINGLLRRDLKFDGVVITDDMQMKAITNYYGLETAVTLSINAGADMLIF
                     GNQLVEKFQDSTEIIDMIEQKVQSGEISEQRINEAYQRIVKMKRSFNY"
     gene            complement(18452..19624)
                     /locus_tag="AC06IO_000584"
     CDS             complement(18452..19624)
                     /locus_tag="AC06IO_000584"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIO9873799.1"
                     /translation="MKAKVILVNGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIG
                     SEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEQ
                     TNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVHVLQELLPHNHIIWL
                     PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKL
                     SRGKRVLDLYTFTGGFALHAAKAGALHVTAVDSSAQAIEQAKNNAVLNHLNTIEFIKA
                     DAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAFKYMKPGSLLMTCNC
                     SSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAVLLALV"
     gene            19702..20685
                     /locus_tag="AC06IO_000585"
     CDS             19702..20685
                     /locus_tag="AC06IO_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873800.1"
                     /translation="MRQFTKYLAIFLFSSSVSVAYAGPWFTGPLLAPAGHTVPKGHTN
                     LEVYGIDVFTDGQYNASGQRISTPKFKTFAANPILTHGFTDWLDVQLTVPYLFNSTRG
                     VHENHLADVSTALGIQILEQKGSPKRIDFRVLLQETIPTGRYDQLNPALLGTDATGLG
                     SYQTLVGLDFQYLLQVFKTHYLRTRFIASRLFSSNVHVDGLSSYGGSLNTHGTVKPGI
                     ENNLDLAFEFTLTQNWVAVMEGYIATGKATRFNGILNFNSIGSPPVNVSDLDAAPVVI
                     GNGSFREEALAPAIEYNFNENIGVIGGVWFPVSGVNTSHYMTYVLAINAFW"
     gene            20713..22587
                     /locus_tag="AC06IO_000586"
     CDS             20713..22587
                     /locus_tag="AC06IO_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIO9873801.1"
                     /translation="MKKNRFTYGLALGALGVVFGDIGTSPLYALKVTLSGIPINQFNI
                     LGVLSLIFWSLIIVVSFKYLMIIFRADNDGEGGILALLALMKHKSTKYQPLFYIVAIF
                     GAGLLLGDGMLTPAISVVSAVEGLGTLSDKLYPYVLPIASVILVLLFSLQAKGTARIG
                     YLFGPLILIWFITIAILGILQIIEHPVVLQAINPYHAIAFLVDEGLRGYFLLGGIFLV
                     VTGGEALFADIGHFGKNPIRFSWFFIALPCLLLNYFGQGANLIVRPEEISNPFFMIAP
                     PWFYLPLIIIATVATVIASQAVISATFSLTKQAVLLGLCPKIPIVQTSMLHSGQIYVP
                     QINFILFIGTMAFCLAFKTSDNLAHAYGIAVNLEMLLVDAMVAYAAVSIWRWSTFNVI
                     FLFGLFLLIDLAFLGANTHKFITGGWVPIVLAFFIAFIMYSWRYGLEYLRDNFYMNKE
                     DISKILKQLQYKSLNQLPGVSAIFITDVYDKSGGSFLHFLKLNRSVPENVLIVNYIVD
                     NIPYVHYSQRYEIVCLDEKVCKLVIHYGFMETINIPRSLEKACNKNILPFKFNVDTAT
                     FMVEIPNIMASKEKRSLSFYWQEKLFAFLMRNYSANLNIEFYKLPYNRTIAIGTYCIL
                     "
     gene            22705..23643
                     /locus_tag="AC06IO_000587"
     CDS             22705..23643
                     /locus_tag="AC06IO_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HIO9873802.1"
                     /translation="MWFNNALMFQYKLDGECDLNRSLTEEALKPCPPHARFTYGWVPA
                     FEQEIVHEVAGSTLICLGKEERILPRGVINKMLKEKIQQIEAAQGRPVKRAEKAQMAE
                     DLEFELLPKSFCVQKKLLAILDSVSQRLIINTSSNNQASQLTSFLRKSIPGIVIEPLN
                     LTENLALRFAEWIHSPATLPDDFQLASDCLLFSLEDEKKRVHCKGYELPAEEILTLLA
                     QGMGTAEISLIWKERIQFTLTQDFTFKKLKSLDYLIDDFNEIRQLEEEYQRQDAALTL
                     LSGELRELINGLMKIFVNHAEMKSAQIEKVAEDCTI"
     gene            complement(23791..24564)
                     /locus_tag="AC06IO_000588"
     CDS             complement(23791..24564)
                     /locus_tag="AC06IO_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIO9873803.1"
                     /translation="MKRIHVLFLIGLVVGISSCTKWGKDDEDNNPYKGMTAKQLYTAA
                     QTSLKKEEYATAAKQLEAMESMYPFSDYTESSQMQLIYAYYKDEDYPSAAATAERFIH
                     LYPRAKNVDYAYYMRGLANFQQTRGVFAKMLPMDESWRDPGTQTQALVDFATLIQKFP
                     DSKYKANALQRMIYLRNMFAQHELNVSLYYFKRKMYVAAIERASYLVKNYPQAPSAQK
                     ALVVMYEANKALGLNKAAEDAMAVYKATYHTSNMVRINS"
     gene            24730..24817
                     /locus_tag="AC06IO_000589"
     tRNA            24730..24817
                     /locus_tag="AC06IO_000589"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24764..24766,aa:Ser,seq:tga)
     gene            complement(24948..27335)
                     /locus_tag="AC06IO_000590"
     CDS             complement(24948..27335)
                     /locus_tag="AC06IO_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIO9873804.1"
                     /translation="MLTDAQMQQAINNLNILIAHHNSPISSFFFDNERVTRNWFFKSE
                     SIHATALSNYLKKIIDEVSSDKVDKTNSLYASLFITLQNFLHIQPNRVIESLNFNLEN
                     SQYLKRKNLIIKRLEHFHIENQFRDDLKRHLLPEIQYDTCTGHYTSPDDDKAHQHGSE
                     AQRIFFSTQWERLKALCNWLLAKMGIHIFETARYKEHDYLDSEVYANDPAIEPGVSPQ
                     QSATHYWIGHASNLMTIPAHDSPLHVVTDPVEGDLAPFLYPRMTKEGSLIDGTGSKKL
                     PKIDVVVISHNHHDHVSQSTLKRLLKQQPKMVVPEGDESFFLGMGFTDVVGLKWWEQA
                     KITDFQGNELLRITAVPARHWSGRSLTDAHCSAFNGYVLHANQLDGDIYFAGDTALMD
                     DMLSQPIFEKFNIVTSIQPGGPDERREDMESTHQSSADAILMHFKILAAQYQKMKEKN
                     ESLSLDDFLNENQHLKTLYNHTATFKLGNLRLKDTYYSYQRMVAAFREDAQWRVDHLP
                     KHEQKVYDHIQLLISNMVFKNNQRLSNRQIADLILGKVVIPKIGQRQPLYFKEKPIKT
                     ETFQYRNLITNRRALLEFDMLLQESIAEANEFFNIKRLILNLLRAYQNPWHVFFSRTH
                     IEVGNYEQDIRNCQSNSDLLAVLNQMERNLGKRNPHGHMQSLIYYAKWVIDFSNKYQH
                     ETLMKFKEYFACQQIRKLADQEIHHAGLIVGFGDRQAKQQRFKVLSNELADLPVDVTE
                     YRKAIDNWLATRVNDGQTTQQLLSENRSSIFAKKVTHGENTMLHIKTTLDLAVSS"
     gene            complement(27427..29319)
                     /gene="ravS"
                     /locus_tag="AC06IO_000591"
     CDS             complement(27427..29319)
                     /gene="ravS"
                     /locus_tag="AC06IO_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavS"
                     /protein_id="HIO9873805.1"
                     /translation="MCIEKILEPIIMSGIKKFNLTYQQVPKIFSLPESVFLSKDVELI
                     PEEVLQQTKVQKNIVAKTIEQIDKDLERTCYVDFDGLTVAQLKSDWLTVNPQGDLALS
                     EGVYPSFSQGDNTGAVIGEILQTKHFSLPLSEEDPKAFMEVTRNTTRICFSYKNSAGQ
                     PSGFSLVAAPNGDSEGNHKGWIISVIQNTVGNYKGRKVSVLASPNYVRSEAQSTVDMD
                     TPDAIERELKRVINNDKVASLIKGIFKEDGTLNADYIRQLASRIKGNRNIDDRDRKSA
                     QFNILIELAEQLDDEKLRHYINSIKADMAADIEFFKDGIYEQHLSELSNKISLSLSKM
                     PSVNKKTTEIGRILNNISERYAQIKMIEDAQRKNPELAHIFKAMAQKIKSDFHDIKFD
                     GTRQDNRNILLLSQFEVASVEQKIRNYYQTNPLVKQYFIDKDKANHHLQVELSRLSPP
                     KNEGFFSRNKDSFGFSIFMGLIVGVSAALFLTGVLAPIGVALIAVGAALITTGLVGSI
                     DIYVNEKEYAKETSHYSNEVKCLRSAHREHVVRLEKNLHEQINLSDFLTPDKPEFLSG
                     IKVNKKKPLTPEKVAHALWLQDKSARDTSVAKNRFSIWAAKQENLDFGIEEVESVIKQ
                     RYVMKS"
     gene            complement(29492..30316)
                     /gene="kdsA"
                     /locus_tag="AC06IO_000592"
     CDS             complement(29492..30316)
                     /gene="kdsA"
                     /locus_tag="AC06IO_000592"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIO9873806.1"
                     /translation="MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCIQLN
                     IPFIYKSSFDKANRSSISSYRGPGFEKGLSILEKVKSQIGVPVLTDVHEDTPLFEVSS
                     VVDVLQTPAFLCRQTNFIQKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMA
                     CERGVSFGYNNLVSDMRSLVIMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALA
                     RAAVAVGISGLFMETHPDPDKALSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF"
     gene            complement(30313..31950)
                     /locus_tag="AC06IO_000593"
     CDS             complement(30313..31950)
                     /locus_tag="AC06IO_000593"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIO9873807.1"
                     /translation="MTKYIFITGGVVSSLGKGIAAASLAAILEARGLRVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTNDGAETDLDLGHYERFVKTTMTKRNNFTSGKIYENVIKKE
                     RRGDYLGGTVQVIPHITNEIKRCIKLGADAFDVAMVEIGGTVGDIESLPFLEAIRQMR
                     IELGSQRAIFIHLTLVPYIATSGETKTKPTQHSVKELRSIGIQPDVLICRSEKPLSMA
                     DRAKIALFTNVEKEAVISLEDANSIYQIPMILHAQHLDEIVVKKLSLEAKQADLSEWQ
                     RVVDMQAVQTMTVKIAMVGKYTELNDAYKSINEALLHAGIHTETKVEIIYFDAEMIEK
                     HGALLLESIDAILVPGGFGERGVEGKIKAIQYAREHKVPFLGICLGMQTAVIEFARNV
                     VGLTGANSTEFNKETLYPVLGLISEWMDADGSKQIRDENTDLGGTMRLGGQYCHLAEG
                     TLARKVYGKPQIIERHRHRYEVNNKYVDSLVKHGLIISGRSADNSLVEMIELADHPWF
                     LACQFHPEFTSNPRDSHPLFKEFVLAARIHHQEKDKK"
     gene            complement(32076..32543)
                     /gene="ribH"
                     /locus_tag="AC06IO_000594"
     CDS             complement(32076..32543)
                     /gene="ribH"
                     /locus_tag="AC06IO_000594"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIO9873808.1"
                     /translation="MREIKTIVSDGVTFPIALVVSTFNERITSALKKGALDRLTELGF
                     KSEDITLVEVPGAVEIPFVAQLLAKKQKVEVIVALGAVIRGETSHYDYVCDQVSQGCQ
                     RVMLDYNIPVIFGVLTTENDEQALARVGGTHGHKGRDAIDCAVSMRSIKQQLQ"
     gene            complement(32551..33759)
                     /locus_tag="AC06IO_000595"
     CDS             complement(32551..33759)
                     /locus_tag="AC06IO_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIO9873809.1"
                     /translation="MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATP
                     EAINFMSRLGCGLICLPMAEELIDKLELPMMARHNKSPYGTAFTVSIEAANGVSTGIS
                     ARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVKLAGLTP
                     AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLIQYRVQHENLIHPVATTQIPL
                     EENGVFTMTVFENQIDSCEHFVLVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQ
                     LELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTVDANLELGLPADDRD
                     YAVAYQILKYLGVEALRLLTNNPLKIASLERYGMKITQRIPLEIEPSRENHSYLKTKK
                     IKLGHLLAID"
     gene            complement(33756..34370)
                     /locus_tag="AC06IO_000596"
     CDS             complement(33756..34370)
                     /locus_tag="AC06IO_000596"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIO9873810.1"
                     /translation="MFTGLIENTGKVLMNRQGENSSRLVVSSSYQDLKPGESIAVNGV
                     CLTLLPADFPELCFDISPETLHVTTLSKLSSGDMVNLERAMLASTRFGGHYVSGHVDT
                     RAKVKSICAVNEFIAMDLDGFIPEHNHYLIPKGSITVDGVSLTINTVSEQTIGLMLVP
                     HTLSMTNLGSLKVGQQVNIEFDYLTRIVAHQLQIIGKLNQEEVS"
     gene            complement(34355..35428)
                     /gene="ribD"
                     /locus_tag="AC06IO_000597"
     CDS             complement(34355..35428)
                     /gene="ribD"
                     /locus_tag="AC06IO_000597"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIO9873811.1"
                     /translation="MHEQFLLAALEQAKLGRGFCAPNPSVGAVAVQNGNIIAQAWHRG
                     AGTPHAEQLLLSQILPQTPGVTLYVTLEPCNHWGKTPPCVDAIINHGIEEVVFSYFDP
                     NPIVAKSNSSEKLREHGIQVRHIPIKAIDVFYKSYAYWTMTHKPRVTVKMAQTFDGKI
                     GKSEGARVVLSNALCEQFTHQMRAASDVILTSAKTVQMDNPRMNARLDSGLVAKPVAI
                     VDRNLSLSDDTIIFSTAKHCHIYHQPQANQAKTYPNSTHYAMPLQNGLMNLEAIITHL
                     GQLGYHDVWVEAGGAIFSALHSKGLVHRTYLYLVPGSLGHQAISAYQQQGIFEKAHTV
                     SWQAMGDNMIACLDWQEDECLPD"
     gene            35491..36927
                     /locus_tag="AC06IO_000598"
     CDS             35491..36927
                     /locus_tag="AC06IO_000598"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIO9873812.1"
                     /translation="MIKLNLNYKLITKKTLPKLFYWGLGIVFFFLAPTTNAGSLSPYT
                     TKELEQLEREFIQLINQSDSVERNPLASQYINSIGKKLAHYGRIKTPYFFIVKSKEIN
                     AFAGPGGYIGINTQLILTSSNESELAAVMAHEIAHVRLHHLYSIIEHQKQMRVPLLAS
                     ILASAALGVLNPTVGMGAMMASVSGFSQDNINFTRSKEKEADRIGIDMLIKAGFNPQG
                     MAAFFNKMQENSRYYYTANIPAILRTHPLDADRIAEAENRTSRLPKKNYTDSLDYALF
                     KELIRVSVASNNKQILDYYEHHCHKKNPATACQYGYVLALLNNNNFQKAAEKLNPLLL
                     QDNSNLNFQIAMAQAELGLSQYQSALSRLAELQNNFPDNYAALMAYAQGLLTANQPQK
                     ATAVLLKGSRQYKEDLPLCRELARAQAESHQKSYAYFTQAQCLLLEGQKRGAIAQLKV
                     AQKLAKKDAYLSARISAKIDEIKFMVEN"
     gene            37206..38141
                     /locus_tag="AC06IO_000599"
     CDS             37206..38141
                     /locus_tag="AC06IO_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIO9873813.1"
                     /translation="MNEIQEFLRQKLNSPLPISRPQLAKLPMGTMEFVTYGNGKPVLI
                     IHGGGGGYDQAVLLFKRYIPDGYQMICPSRPGYLGTPIDTGQTAEEQADALAGLLDYL
                     NIPGAVIVAISAGGLSLYPFAIRHPKKTKAIIAIDAIAGEYLMPEQSNKLAQALFMTD
                     FGLWVAKKSMIYFPKSFIKNLIKLEGYLDPVRVDDRVKEILNCRERLEIIAELMNSMA
                     DYKARKTGTVNDMAEGVKTSWYDFSKINCPALIIHGTHDADVKFYNGVFAYERLASKE
                     KERFWIEYGTHFGFFFAHQARKAQQKFREFVIKHS"
     gene            complement(38336..38581)
                     /locus_tag="AC06IO_000600"
     CDS             complement(38336..38581)
                     /locus_tag="AC06IO_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873814.1"
                     /translation="MNSWNSVVFLKTKNAWSDSAAIAKMNGVQSLWSTSGDWDWCIKL
                     DSKHSSPEETALFVKNLRNADWVAETKTSWWKEVAVK"
     gene            complement(38987..40315)
                     /locus_tag="AC06IO_000601"
     CDS             complement(38987..40315)
                     /locus_tag="AC06IO_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIO9873815.1"
                     /translation="MTKSKVLVIDDEPDIRELLTLTLSRMGLTCDAVSDFKQGIEHIK
                     QNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQMPVAVITAYGNVEGAVNTLKAGAFDYV
                     SKPIDLKMLRDLVKTALSMQNPPTENPDEDLLVGESLVMQQLRENIHKLARSQAPVFI
                     QGASGVGKELVAQLIHVNGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDK
                     TGLFVAAHGGTLFLDEIAELPLAMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHK
                     NLIEEVNAGAFRQDLYYRINVIELRVPTLMERLSDIPCLTEAILRKLSKNQNRNLVKI
                     NPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTS
                     TNLNGYLQDQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD"
     gene            complement(40312..41898)
                     /locus_tag="AC06IO_000602"
     CDS             complement(40312..41898)
                     /locus_tag="AC06IO_000602"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIO9873816.1"
                     /translation="MSAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKY
                     YPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFLSGTIDVIAISSMLGIIGNLESG
                     YGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYG
                     AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRI
                     RLMNTAAREFFNLSKHHAIQKLDQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRV
                     HFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAHELRNPLGAIAHAIQ
                     LFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRREKSQPQVNELTTFLEHFKQ
                     DFTHNNPCDLTIKLPVNKPLSVVFDKSQLEQILVILCENAIQHGRDHEGNVNIVIAAK
                     CSSNKITLTVCDSGPGIPSEHRDNIFEPFFTTLRGGTGMGLFIARDLCEINQARLNLV
                     KSNKGCCFAITVNPSDELLI"
     gene            complement(41997..43481)
                     /gene="lidL"
                     /locus_tag="AC06IO_000603"
     CDS             complement(41997..43481)
                     /gene="lidL"
                     /locus_tag="AC06IO_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LidL"
                     /protein_id="HIO9873817.1"
                     /translation="MQGGMMKQLVVAGFFLLLLTSPLSALDVDTYRSSQKQFNTEDKA
                     EQGDAQAQFELGLKYEKGDGVNKDLKKAIYWYQKAADQGQAEAQNNLGVLYLKGEGVP
                     QNSQQAMHWFKKASEQGLAIGQNNIGILYENGLGVKKDLGQAFIWYQKAAEGGNSDGQ
                     YNLAVMYMYGNGIPKDIKKAIHWYIKAAEQGDLDAQNNLGVLYERGEEVPRDLKAAIS
                     WYTRAANEGSLIAQTNLGVLYMTGDPSIQDGKKAIYWYEKAAAQGGEKAQNNLGYIYE
                     QGIGTEKDMKKAIYWYEKAAENGFTLAQNNLGVLYSNDGELQDYKKAYLWFKKAADQG
                     FAEAQNNLGLMYMKGNGLSVNYHEAVLWYKRAAEQGLPLAQHNLAIMYMKGLGIKKDN
                     KLAIKWYQKAAEKGLDLAQNNLAVMYIRGEGVKIDFKKAMYWYQKAAEQGLDLAQINL
                     GIMYLDGMGVNKDFAKAKYWIGKAKDSGSQDALTIWNKNKLWKY"
     gene            complement(43631..44050)
                     /locus_tag="AC06IO_000604"
     CDS             complement(43631..44050)
                     /locus_tag="AC06IO_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1171 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873818.1"
                     /translation="MRFQDSDFEERYNTMWNKIAVSADAQIRQLFGAKGFFSEQQPNY
                     YQLLVNYAQAAKNIVDNLNRQSPMFDDKEYVEGYMIATLQSVYKDFSQYKPRIAGRYG
                     EHSSCVELINKTLDWVQSFDLKLENLSESDDEMKITF"
     gene            complement(44109..45185)
                     /gene="amrS"
                     /locus_tag="AC06IO_000605"
     CDS             complement(44109..45185)
                     /gene="amrS"
                     /locus_tag="AC06IO_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIO9873819.1"
                     /translation="MFDTIPTKYWHKLEDGRIQCDVCPRNCKLREGQRGLCFVRANLD
                     NQIKLTTYGRSSGYCIDPIEKKPLNHFLPGSPVLSFGTAGCNLACKFCQNWDISKSRD
                     IDTLADTAMPEDIARAAQNMNCLSVAYTYNDPVIFMEYAIDVAKECHKLGIHSVAVSA
                     GYINPEPCKELYSFMDAANIDLKSFSEDFYYKITGSHLQPVLDTLIYLKKETSVWFEI
                     TNLVIPEENDSDAELERISEWIMTNLGPDVPLHFTAFHPDWKMMDKVPTPFSTLQRAR
                     EIAIKTGLHYVYTGNVHDTTGSSTYCPHCKLPVIVRDWYRILDYRVDDTGHCLNCRTQ
                     IAGVFKGEVGHWGPRRVMVKLGCS"
     gene            45257..>46462
                     /gene="amrB"
                     /locus_tag="AC06IO_000606"
     CDS             45257..>46462
                     /gene="amrB"
                     /locus_tag="AC06IO_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIO9873820.1"
                     /translation="MSVRQPAVAGYFYPDDPLTLKQTVLNFLDQAPIHKPAPKAILVP
                     HAGYVYSGSVAASAYASLRDKKNSINKIIILGPAHRLYFKGIAYDPVDKFATPLGEIQ
                     QDKELLTQIIDLPYVYSLPEAHQNEHCLEVQLPFCQMIFSKFKILPLVVGETNPQDVA
                     RLIARVWGGDDTLLIISSDLSHYLPYHIAQREDKKTCLSIDTLNGEEILHDAACGYFP
                     LRGFLYFARQHHIYSRLLDLRNSGDTAGDKERVVGYAAYHFYQKLNFSEHCADELKYI
                     AKETIRLQTEENKALAINYNDYNQLLQIRIPTFITLKKNGLLRGCMGSLIAKEQLADN
                     VIYNSIRAATADPRFPQIRPSELKELSLTISLIKPLSPLYFDSEEELKSQLQIGIDGL
                     VLIYGSYQAT"
BASE COUNT        14403 a         9572 c         8199 g        14288 t
ORIGIN      
        1 gcttaaaaat tagctatgat attggctgaa aaaacttagt aaaaaacttg gagtttctat
       61 ggaacaatgg catcaattta ctctactatt ccctttaatc attttagttt ttatttcatt
      121 aattagtcct gggccagatt ttgctatcac ggtcaaaaac agcttagctt attccagaag
      181 gagtggttta ttaaccgcgt taggaattgc tttgggcagt ttggttcata tcagttacac
      241 tcttcttgga ttaggcctgt ttattactaa aacacctgta atactgcata tcatccaata
      301 tctaggggct ggatacctct tttatattgg ccttaaaaat cttggtgcca agccaaatgc
      361 tgaccaaata ggaagttata caacgtacaa cgatttaaaa ccaagtgaag cttttttcaa
      421 tgggtttata acgaatttat tgaaccccaa agccatgctc ttttttatca gtttgttttc
      481 ggtacttatg cccagagaaa tttcaatctc caataaatta ttcctggcaa ttatcctttt
      541 tctacaaaca ttgttatggt tttcttttgt tgcattcagt ctttctggaa aaaatacgcg
      601 cacgaaattt caaagagtta gctattggat tgaacgtatt acaggtgtca ttttaattgg
      661 attggcatta aagttgctat taagccaatt agggtctgtt gatatttcac ctgcttgagt
      721 cgaaaagcct tgattttcga gcaccgtagc ggagcacaga aaatcaaggc ttttcggtca
      781 agatagtaga aatgtcaaca gaccttagtc taatggccgg ataaaacata tacaaaatcc
      841 atcaaacttt tattaaagga ttttgtatac gatataaaac atgctttgct aacctatgat
      901 cagaggctaa tttgggatgt aaaaagtctc ccgcaatatc tctgatcatc ccaagtctat
      961 ccataacact tcttgagcgg gaattatcag gcacagtaaa agaaactatc tcgggaatat
     1021 gcatctccag aaaagcatat tcaagagcta cttttgcccc ttccgtagca tagccttgcc
     1081 cccaaaactc agttgccaaa cgccatccta tctctacgca aggagtgaag tgagcattcc
     1141 aagttggagc atttaatcct ataaaaccaa taaactgctt acagtccttt acttcagccg
     1201 cccacaaagt aaagtttttt tgagataatt gttcattcat tctgataatg aaatctttga
     1261 ccatatccat agaccacaac ccggggaaat acttcatgac ccgaggatcc tgactcattc
     1321 tatagaaagg gatagtatct tcttcatccc acgctcttag caaaatcctt ggggttgtta
     1381 aatatttcat ggcaaatata ttcttccttc caaataaaga tggcaatttc catagagcaa
     1441 tactctgttt tccttcactt cacaataaag atcaccttgt ctgaggcctc cttgtaaagc
     1501 agttatatgg ttcttcctta actgttcaca ccaataagga gcaataacac aatgagcaga
     1561 tcctgttacc gggtcttctg gtacattaca tccaggataa aaacaacgag aataaacatc
     1621 ggttgatatt cctggagcag ttaatattaa ccccctatag tcaagatgag ccacagcatt
     1681 caaatcaggc tttgcctcaa ctacttcttc tgcattttga tacacaaaca tcaaatcaaa
     1741 ttggcttcgc aatgttttaa atggcctttt taaattcaaa tgctgcagtt cagcactgaa
     1801 attaatttct tcatagggca gaactggaaa gtccataacc aagccttgac ctgtgttttt
     1861 aacccatagc ttaccgctca aactattaaa tataatttct tcccctgaaa agcccaattg
     1921 attaaaaagc acataagagc tggcaagagt ggcatggccg cacaatgcca cttcttcatt
     1981 gggggtaaac caccggatcc tgtatccatc cgtttcggga acaaaaaagg ctgtttcaga
     2041 taaattgttt tcagctgcga tggcttgcat agtctcatca cttaaccatt tatctaaagg
     2101 acaaacagcg gcaggattac ctttgaataa atttgaggca aatgcatcca cttggtagat
     2161 tggtaagctt ttcataaatg tctcctaaaa caacttgcga ataataacat agcctgttat
     2221 tatatagcaa gctatttaat aatgagaaat ttatgtatct ttcctctctg ctaaaaaaaa
     2281 caaatcgttt gttaatcaag cgagccaatg aattaattaa accctatgct atgagtcatg
     2341 cctacactta tatattaatg gaactgtata ttgaagatgg tttgacacaa tctgaattag
     2401 ttcaattgat tgaggtagaa caacctaccc ttgtaagaac tttggatcga atggaaagag
     2461 acgggtttat tactcgaaaa ccaagctcaa tggatagaag agtcgtgcat atttttctta
     2521 cagaaaaagc cagacttttg caagaaaaaa tagatgaatg ctccagaata ctaaataatg
     2581 agattctggc tggtttttca gaagatgaaa aaacaaaatt acaagcgcta actcatcgcg
     2641 ttcttgataa tttaaggaat cccattgaat aatttataat gatatttcta atcgcttcat
     2701 tctatagcgt caccaatgtt ggctttacta atttttcaaa atcaccaaaa gagagttgca
     2761 tcaattccga atgagaaccc gcattgaata agatattatc ctgcccactc agtttagtcg
     2821 acaccaatac tggtaaacca tacaaattac cgaatggggg catcgctcca acatcacatt
     2881 ccgcaaattt cccttcaaac tctgattcag tagcaagttc aagatcagaa gtaccaatag
     2941 cttctttaag tttcataaat gtaatatggt cacttgcagg taatactacc atggctaaac
     3001 gtccatccat ttttataata accgtctttg ccagttgttt tcctgaaaca tgagcagaag
     3061 cagctatttc ttgtgcggtg tacgctggtg aatgagctat agacaaatat tttatcttat
     3121 ggctgtccaa aaattgttta agctttttaa ctggcatgac aaactccttt tcgatactaa
     3181 ttcattcttg cagaaaatgc cctctctcaa gtgcccttat ttaaaatttt gaatcatttg
     3241 tttgcaagca gtcatttcgc ctaaacaatt actcaacagc aaactgcaaa ttaccaatag
     3301 gcctctgata ctcctgttag ttcataatat ggcgtttacc ttcaacgtaa accgtacttg
     3361 cagcagcctc ctgtgtgttt ttaaccgttt caacaaatcg tactcggtca ccaacctcta
     3421 atttattgat gtcataatca acgacacttt ggcctgtaaa tcgtatcaga cgcccatcaa
     3481 ttgtttctat gtagccataa tcagcaatgg ggcatacttc aagaactctc ccaccacgaa
     3541 tagaatcctc atgacgcttg acatggcctc gccgttgatt tgcataattt ttaagctggc
     3601 gctcaatcgc attaaaagcg tctcttagag aaacatatac atcttcatgc gcatgcttgt
     3661 ttgatgattc acggctcaca actaactcag catcaggaac tgtcagatca atacgtacat
     3721 gataaagctt tcctttgtta tgatggcgat gaggagactc aatgactact cgacaaccca
     3781 ttattttgtc ataaagacaa tcaagctttt tagcaagttt tatagccctg ctttcaacag
     3841 cttttgatgg atccatattt cggaatgtca cctgaatagg aagtaacatt tttaacctcc
     3901 cttatgtttt gattatgaat aatttcagat cttatttact aatctataga caaaaaaaat
     3961 gttgctttca atagaaaaaa gataagcatt tgaattaaca ataaaaataa aattattaaa
     4021 aactagacta aaaaaatggt ataaaaaatg aattgagttg acatgcaaat acttcataac
     4081 tgaaacttaa tgagcatata acaacaggac acagatgctt ttttgggagg gattgcatac
     4141 gtggataagc ctaagagttc gacgctgcac tccgaagcaa gatcgcttca gaccattctc
     4201 tttaacttac actcgtcctc aagtcagcca ccttctccat tcattttaga tttggaacag
     4261 ctgtactttt tgactcggca gtagactata aaattaccgg attttatgtc aaactctcag
     4321 gtttctataa acatgctctt aagtacttta acgaggcttt aaaaaataaa gcgtagcctc
     4381 accagataaa atcatctggc ttattcttct gtgtgtactg attcgctagc cactaccaca
     4441 ttttgtgctt tcaaaccctt aggtcctcga tcaagttcaa aggtgactgg atcattttca
     4501 tgcagcgttt taaaaccaac tccctgaatg tctttgtagt gcacgaaaat atcttcgcct
     4561 ttttgattaa caataaaacc aaatcctttt ttttcattaa accatttaac atggcctgtt
     4621 tctctttgcg acattcgcat ttcccctttg acttacacag atacatcaaa attgttattt
     4681 aaataaataa aagttcactt tagcgcaact taaagtgaaa aaataaatca ctccctaatt
     4741 agtaatttac gctataataa acccttttcc atgcaaggat aaatcctgca ctatgattag
     4801 catatcagtt ttttgttgat tgttaaggat ttttttaaaa tatgtgattg ttttcaataa
     4861 aattttgtat taggaaacca gaatgaatca taccaaatca tcttttataa aattagctct
     4921 ggagtgtcaa gtacttaaat ttggtgaatt tactttaaaa tcaggacgaa taagtcccta
     4981 tttctttaac gctggtttat tttatcatgg taattccata cgtcagttag gtcaatttta
     5041 tgcaaaaaca cttttagaac aagaaatttc ttttgagcac ttgtttggac cagcgtacaa
     5101 aggtatccca ctcgctactg caactgcagt agctttagct gagttaggca aggacatcac
     5161 agtgactttt aatcgtaaag aagtaaaaac tcatggagaa ggcggacaat taattggttc
     5221 accgctaaca ggaaggacag tgatcattga cgatgtgatt acagcgggca cagcattcag
     5281 agaatcacaa accctgatta aagagaatgg aggaattctt cgtggtgtca ttatcgctct
     5341 ggacagatgc gaacgaggat taactgagaa atcaaccctg tcagaaatca gggagcaagg
     5401 aattgaagtc tattcaatta tcaatttgtt tgatctaata gaatttctga aaaatgacaa
     5461 tcaatacgaa caggtacaaa aactggaatc ttatcacgag ctttatggcg catattagga
     5521 tcaggactta cgaaaaaccc caatctcttt gttaaaaaat gtttttaatt cggtcattta
     5581 gtgtatctaa actctctcat taaaaacatt tttttcctcg accttagagt ttttcataag
     5641 ttctgaggat tactgatttt cctcaaaaaa tagtaaaaaa agccatattc attgtgtgtt
     5701 aagttgctga gctatccatg aaactcagca acttaacact tagtttgtct tcgtctactg
     5761 cttcacatct tcttctctaa gttccaacca tactgcatta agcaccgcaa atacacaagc
     5821 cagccctgtg cctaaaatcc aggagaaata ccacataagc aaaacctcct aatacgcaga
     5881 atcttgttct gctttaatag aactttccgt taccttacct cgtaacactc gataaaccca
     5941 agctgtgtac gcaagcacaa taggcaataa taataccgtt gctaccagca taataaacaa
     6001 agtaacctga ctggaggagc tatcccaaac gattaaactg tatttaggat ttgaggaaga
     6061 aggcaatatg aaaggaaaca tactaacccc taccgttgca atcaaagttc caacactcaa
     6121 tccactcagc acaaaagcaa acacactttt attcgcaaag ataatcgcta aaaaagcaga
     6181 tactatacat aaaacaggta ctaacagtga cacaggatac tctttataat tgttaaacca
     6241 tgccccagct tgaatactaa catccttata ggaaggattt gatggcccat catgaggaac
     6301 cattcctttt aaaacataac catcaatacc ataataagcc caaatcccta atccaataaa
     6361 tagcagaata actaaaatag cactccatcg agctctatct tttgctttat tttgcaaagc
     6421 cccaaccgtt tttacttgaa taaaaaaagc gccatgcatg gcaagcatga acacagataa
     6481 taatccacat cctaaagcaa acggattgag taattcaaaa aaacttcccg tatagaatac
     6541 tcttaagctc tcatcaaaat aaaacggaac accttgtatg acatttccaa ccgctacccc
     6601 aaagattaaa gccggaacaa atccgccgat aaataaagcc caatcccaaa atgctctcca
     6661 tgtgggatta tccagctttg acctgtattt aaaaccaacg ggtcttaaaa tcaaggctaa
     6721 aagaacaacc agcatcgcca tgtaaaagcc cgagaaagat aaggcataga gagtgggcca
     6781 agctgcaaaa atagcgcctc cgcctaatat aaaccaaacc tggttaccct cccaggtcgg
     6841 gccaatgctg ttaattaaaa tccgtctctc aatatctgtt ctgcctaacc agggaagcca
     6901 cattgccaca ccaaggtcaa atccatccat aatggcaaag ccaataagca aaatacctat
     6961 taacaaccac caaattattc tgagtgtttc ataatccaaa ggcatttgat tctcctttca
     7021 tcaatgatga ttcataccat ctgggccaag acgtatgtat ttgaccatta aataaatctc
     7081 aacaattgca agaacagtat aaaacaatac aaagcctgca attgatgtca ttacttgccc
     7141 tgatgttaaa gaggaggccc ccataaaagt aggcagaata ccctcaacaa cccagggttg
     7201 acgaccatat tccgccacta tccaacctaa ttcagccgct acccatggta aaggcaaaga
     7261 ataaaatgca attctgtgat accaggttgt cttttccaac ctatgcctta ccgataaata
     7321 aaaaccggtt gcgaaaagaa gtataaaata tatcccacaa gcaaccataa ttctaaatga
     7381 gaagaacatt ggcaggactt taggtttgag atcgtgcgcc gcttgattta tttgctcttc
     7441 agtggcatcc gtcacattct cagtatactt cttgagcaat aaagcataac caagattttc
     7501 gctatgtttt gtaaaaacag ccttagcttg ttcattcttt ggatctttct gtaaaacctt
     7561 caatgcatcg tatgcgatca tcccatcttt gatttttaat tttccttcct caattaattc
     7621 aataatacct tgtaaaggag tattgaacga acgcgtggca ataataccaa gggcataagg
     7681 aatatctatt gcatattttg tcgttagagt cttctcatct ggtattccaa aaacagtcaa
     7741 attggcggga gctttctcgg tatgccacat cgcctccata gtagccaact tcattttttg
     7801 gttttcattg gcaacataac cgctctcatc gcccagtacc acaacagaca atgccgaagc
     7861 caaaccaaaa gaaactgcga ccgtcattga gcgtttggca aacgctttat ttcgcccccg
     7921 caaaagaaaa taggcgctta tggataagac aaacatggct cctgttacat atcccgcact
     7981 gatggtatgc acaaacttgg cctgagcaac aggattaaac agcatttcgt agaaactgct
     8041 aacctccatt ctcatcgttt ggtaatcaaa ataggcgcct acaggatgtt gcatccaacc
     8101 attagcaatt aaaatccaca atgctgaaag attagtgcct aaagctaata accatgttga
     8161 ggtcagatgc tgcaattttg acaatctatt ccagccaaag aaaaataaac ccacaaaggt
     8221 agcttccaga aaaaaggcca ttagcccttc gatcgccaat ggagcaccaa atacatctcc
     8281 cacataatgt gaatagtaag cccagttagt accaaactgg aattccatag tcagaccggt
     8341 tgcaactccc aaaacaaaat taatcccaaa caaaacaccc cagtatttga ccattttctt
     8401 ccagagatca cggccagtca ttacataaac tgtctccatg atagctaaca taaccgataa
     8461 ccccaacgta aggggtacaa atagaaaatg atatagtgca gttaatgcaa attgtaacct
     8521 tgataaatca acaacttcag cagctggaat cataaaacta cctcttgtta tttggggtaa
     8581 gtactgtttg ttcttctttt cctaacaacc attctttatt actagacaac tcaggatgca
     8641 ttcctctgac acagaaatac cataataaaa cgagcaatat cattttaaat gaaagggtaa
     8701 atacaatatc tctggtaagt ggtttcatga catttcctca ggatttatca tatcgacagt
     8761 ctctgataaa aatatttact tctgcctttt gattttcggt caatccagtt tcccggaaag
     8821 agacctgtat actctacccc atccagaatt ttttttcact aaattattga caatgctaaa
     8881 gataatcatt atcatcatta acatgttaca atacgttaat acattaacat gaaaatacat
     8941 ggatctttta aaaaatctgc gctgcctaat ttgatgcaag ctatcagctt gcttaaaaac
     9001 gaagacgaag ccatgcagtt ttttacagac ttatgtactc ctgctgaact agaatctatg
     9061 gctgatcgat ggcaagtagt tcctttgcta cgccagggca tcccatacag aacgattcat
     9121 gaagaaacag gggtcagtgt aacaaccatc acgcgagttg cgcgttgttt aagctttggt
     9181 acaggcggtt atcgtttaat agccgaaaga ttggagaagt tgtgaaaaat cgtttacgtc
     9241 tggccttaca aaaaaaaggc cgattatcag atgaatcttt aaatctcctg caacgctgtg
     9301 gtttaaagtt tcgtattaaa ccaaacgcat tattaactca tgttgataat tttccaatcg
     9361 atttactttt tgttcgcgat gatgacatcc ctactctcgt attcgacagt ttatgtgatg
     9421 gtggcatcgt gggagaaaat gtgctgctgg aagcttcttt taacagacca gacaaagcct
     9481 atagaaaaat attagcatta ggcagttgta attgtcgttt atcgatagca ataccagaag
     9541 cattcgacta tcaaggagcc ggtagtttgg atgggaagcg aattgcaacc agctatccaa
     9601 atttactcaa caattatctt gctgaacgaa aaattagtgc ggaaattctg acattatccg
     9661 gctcggttga ggtagcgcca cgaatgggaa tggctgatgt catatgcgac ttggtatcca
     9721 ctggccaaac actcgaagat aacaaactca aagaagtcga tactgttctt aacagtcagg
     9781 ccgtctttat tcaaactcag agacaattgg atcctgatat tcaagatttg tttgccatgc
     9841 ttgccagaag aatccaggct gtccagcaag ctcaggaaag aaaatacata gtttttcatg
     9901 cgcccaaatc cgcccttgag cgaattgctc agaaattacc aggcgctgag tccccaacca
     9961 ttcttccatt accgggcaac aatgaaaaag ttgcagtgca tgtagtctcc agtgaaggtg
    10021 tgttttggaa tacgttagag acaattcaat atttaggtgc cagctccatc ctggtattac
    10081 ccattgaaaa aatgctggag taattcatat gttaacaatt aaaaactggc aattactttc
    10141 tgaaaacgat aaaaagctat gcctttcaag accgcgacag agttctgcaa taaaagaaaa
    10201 tgtgctggaa attataaatc aagttcaatt atcaggagat aaagcacttt atgatttaac
    10261 taaacagttt gatagattca atttacaata tttacaggtt cctcaggaaa aaattgaaca
    10321 ggcaaacata ccgcaaaatg ccctcaatgc tataacacaa gccattggaa caatctcgtc
    10381 atatcaccag tctttgttac ctgaaaatac cgagatttct actgcatcag gaattacaat
    10441 ccgcaatgtc tacagaccaa tccaaaaagt tggcttgtat gtgcctggag gaaataaaac
    10501 ccctttagtc tcttcgcttc tcatgcaagc aataccggca aaggtagcgg gctgcccaat
    10561 aaaagtgctt tgtactcccc ctgatgcaga aggggaaatt aacgaacata tactcgtagc
    10621 cgcaagatta tgtggtatcg acacaattta cgctataggc ggagctcaag ccatagcggc
    10681 tatggcttat ggcactgaaa gtgtcatcaa agtggataaa atattcggac cgggtaatag
    10741 ctatgtcact caggcaaaaa ctttagttgc aattgacgcc gacggagctg cgattgacat
    10801 gcccgctggg ccatctgaag tgatgattct tgcagatact gaggcaaacc ctgaatttat
    10861 tgctgcagat ttattagccc aggctgaaca tggcccggat tcacaggtta ttttaatttg
    10921 tgatgaatgt gaattggcaa accaggttaa tcaacagctg gaaattcaga tgagttattt
    10981 gtcaagaata gaatttatca aacgatctct cgcgaatagc aggataatta tctgttctaa
    11041 tcaatcggaa cagctggata ttatcaactc ctacgctccg gaacacctga tcattaacag
    11101 aaaaaacccc gaaccatggg ttgagaaaat cgtggcttcc ggaacagtat ttcttggctc
    11161 ttgggccgca gaaacaatgg gggactatgt aacaggaagc aatcacgtct tacctaccaa
    11221 tggttttgcc agaaaccata gcgggctttc tactcttgat tttatgacac gatttaccgt
    11281 gcaagccata aatcaggagg ctatccgtaa tttaggtcca gcggcaatga ctttagcgga
    11341 attagagggt ttggatgctc atgccaatgc cgttcaaata agactcaata ctctggggga
    11401 ttaaatgtcc atactcaatc ttgttcgtgc agatctatta aatagtcaaa attatgtccc
    11461 cggtggtgag agtgctcgct atcgatcaca tgccaatgaa ctgccttggt caccagttac
    11521 tatgggggag cacaatttaa attattaccc aaacattggc ttacaaatag agctgcaaaa
    11581 gcaattggca aaacgttatc aaatcaattc cgatcaaatc atactgacta gaggaagtga
    11641 tgatggcatt gatttgacaa ccagactttt tctaaccgcc ggaaaagacg ctttcatgca
    11701 atttccccct acttttccta tgtacgcttt ttatgttcgg ctacaacaag cagaattgat
    11761 cgaatgtcct ttggatagaa ggactaattt tcgcctcacc ctggatcaaa ttgaaaatag
    11821 ctggaaacct aactgcaaag ttatcatgtt ttgtagtccc aataacccga ctggtaattt
    11881 ggtggattta aatttaattg ccaagacatg tgaactgtat acgaatcaat ctattatagt
    11941 tgtggacgag gcgtatatag agtttgcaaa tgcaccaagc gctacgtcct taattggaga
    12001 gtttgaaaat ctgattgtct taagaacctt gtcgaaagcc tttgggctgg ctggattacg
    12061 tttaggctgc attatagccc agagtcctat tattcaagtt tttaataaaa tcattgcccc
    12121 ctattccatt gccactccta gtatggatct ggctaaaaga gctttgaata atagcgattg
    12181 gttcacaaaa actattgagc aaatcaaatc atctcgtgcc tgggttataa aaaaatttgc
    12241 cgataatcca atcattgaaa aaacttatcc tacggaaacc aatttcattc tcattcaaac
    12301 acgattttca aaacaattag ccacttggct tgcccgttat ggcatcgcag taagagattt
    12361 tccttcgtcc tctttattgc atgatcacct tcgtattacg gtgggtcatg atgagcaaaa
    12421 tcaattattg attgatgctt tatcctcatt taacgctgat gtagcaggat taaattatga
    12481 aaaagatttt atttattgac cgcgatggca ctttagttga agaacccttt gattttcaag
    12541 tagattctct ggataaaatt aaattaactt ctggagtaat accagcccta ttgcaattac
    12601 aaaaagctgg ctttaccttc attatggtat ccaatcaaaa tggcatagga accgtagctt
    12661 ttccagaaga ggattttgct gtttgccatg aatttattct ggatctgttt tcctctcaag
    12721 gcattctatt tgatgagatt tttatatgcc ctcacacccc tgaagacaat tgtatttgta
    12781 gaaaacctaa aactggcttg ctcgaatctt acttaaaaga aacagcattt gccaaacaat
    12841 attcatgggt aattggcgac cgggatactg ataaagagtt tgctgataat ctgggcgtta
    12901 atttcttgcc catttcgaaa acacatactt gggaaatggt ggcttctgca attatcaacg
    12961 atgctcgcaa agcgtctgtg caaagaaaaa caaaagagac ggcaatagat ttgtcagttc
    13021 aactggatag cgatcaaacc agtgttatag atacccctat ccctttcttc actcacatgt
    13081 tggaacaagt agcaaagcac gggggctttg atttacgatt acaagcttct ggagatttgg
    13141 aagtagatga gcaccacttg attgaggata cagccattgc attgggggag gcaattagaa
    13201 cagcgctagg tgataaatgg ggaattaatc gttatggtta tacccttcct atggatgaat
    13261 cattagctac cattgcaatc gatattagcg gcagaagttt ttgtgacttc aaaggtcagt
    13321 ttactcgtga atttattggc ggtatggcaa cagaaatgat ccctcacttt ttccaatcgc
    13381 tatcgagcgc gttgggggca actatccata tcgaggtaac tggcatcaat catcaccaca
    13441 tgatcgaagc atgcttcaaa gttttaggca gagccttgcg acaggcatgc tcaagaacga
    13501 ataattatct accttcaacc aagggtgtat tatgattgct gtgattgatg tcagtggaaa
    13561 taatttaact tctctgacaa atgccttgat tcgattggga gggcattttg cactgaccca
    13621 tgatgctcaa gaaatacaaa aagcaagcca tgtcattttg ccaggggttg gaacagcacg
    13681 ttctggtatg acagctttgc agcaaaatgg gctaatagac gttttaagaa ccttaaccca
    13741 accacttctt ggcatctgtc ttggcatgca attattgctt gaatacagtg aagaagacga
    13801 cataccctgc ctaggtttga ttcctggagt tgcagagtta ttaaaagctg aaagaaatca
    13861 tcctgttcct catatgggat ggaacaacct tcattggcaa aaaacatcat cattacaaca
    13921 aggattaaat aatagtgatt atgtttattt tgttcatagc tatgctctaa aagcagatga
    13981 ttatgcattg gctcgatgta ggtatcacga agagttcact gcagttgtaa aaaaaggcaa
    14041 tttttatggc atgcaatttc acccagaaaa atcagccgat gttggcatgg tattactaaa
    14101 taacttttta tctttggagt ctaaatgtta gttattccag ctattgattt gcaatctgga
    14161 cgatgtgtgc gcttaaaaca aggacggttt gatcaagtaa cgcaatttag cgtttttccc
    14221 atagaaaggg ctttacattt tgccaaacta ggcgccaaac gccttcacgt agtcgatttg
    14281 gatggcgctc gcagcggaaa aatgcaacag ttggagctca tttgttccat gcaaaagaca
    14341 gggatagcaa ttcaagccgg aggtggaatc aggagtatag aacaagcttt ggaatgcagc
    14401 aacgcaggaa tttcacaatt ggtgattggc agcctggcta ttacaaatcc tgatttgaca
    14461 atacaaatca ttgaaaagat taaacccgaa aatattgttc ttgctctgga cgttcgtgtt
    14521 gatacaaaag tcccccttct tgcgattaat ggctggcaaa ataatagtac aagcagcctt
    14581 tgggaagtag tgtcctatta cgaaaaccat gggataaagc acatactttg tactgacatc
    14641 gcatgcgatg gcatgatgaa tgggcctaat ttcgatttgt accaacaagc agttgaatat
    14701 tttcctcaga tcgcctggca agcctcaggg ggaattcgcc atatgcagga tattacaaca
    14761 ctgggttcat taggaatatc cgccgtaatc cttgggctaa tgctttatca agataatgtg
    14821 aatttcgagg agttattatg ttaaccaagc gaatcattcc ctgtcttgac gttcgggata
    14881 atcaggttgt caagggagta aaatttcgca accatagaat aattggagac atactcccat
    14941 tagctgctca ctactcagca tcaggcgcag acgaattggt attttatgat attacagcaa
    15001 gtagcgatga ccgatctgtt tgtcctgttt gggtcaataa agtggcggct gcgatcaaca
    15061 ttccatttac agtggctgga ggcatccgaa ccttgtacca agcaagatca attttaaatg
    15121 ccggagcaga taaaatctca atcaacagcc cggcgttgga aaacccttca ttgattaacg
    15181 aattgagcaa agaatttgga aaacaatgtg tggtaatagg agttgatagt cagtggatag
    15241 ttgatgatta ttatgtctat cagtatacag gcaatgaaaa taaaagtatc aattcaaaac
    15301 gcaaaactcg agaatggata tatgaaattc aagaccgcgg tgctggtgaa atagtcctta
    15361 attgcatgca atctgatggc gttcgaatgg gatatgatat tgcacagcta tcagaaatga
    15421 ttaacctctg ccacgttccc ctcatcgcat cgggcggtgc tggaaaaatt gatgattttg
    15481 ttgaagtatt taaaaagtct cgtgtcagtg gggctttggc agcaagcgtt tttcatgaca
    15541 aaatacttgc aatcaatgac ataaaactag cattacaaac agaaaatatt gaggtaagac
    15601 tatgagtgac ttggacatta actctcttga ttggcaaaaa atgaatggat tattgccggc
    15661 aataatccaa aacgcagaaa atggtaaagt attgatgcta ggttatatga atcaggaagc
    15721 attaattgcc acactaacta caggacaatt gaccttatac agcagaagtc ggaaaagact
    15781 atggcgtaag ggagagtctt ccggcaatag tatggctctg caacatatta gtgccgattg
    15841 cgacaatgac agtcttttga tccaggttat tcctaaagga cccgcttgtc atttaggtta
    15901 ttcaacctgc tatcagccac ataataatta tactcttggt tttcttgatg gtttgattga
    15961 attaattaat gaacgtgctg aggaaaaaaa tccaaacagt tatactactc aactcttgga
    16021 ttctggtgtg aaccgatgtg ctcaaaaatt aggagaagag gcggtagaaa cagtgatcgc
    16081 tgctgtgagt aacaataaag aagaacttat caacgaaacg tctgatttaa tttatcattt
    16141 attagtgctt ctcaaagctt gtgaattaag tttttatgat gttttattgt gtttacaggc
    16201 aagagacaga agtgtacata cttaattgct tattccatag aagatctcta taaccagaat
    16261 ccccagtaat tatcaatcaa gactagttaa ttttttaaac tactattaag aaattaactt
    16321 ttcttgaaat actctttaat gtaatcaggc aaattcattt ccaacactgc ctcatccttt
    16381 ttagttgcat agtaagaagg caactcactc accatagcag actgggaata cagcttgcac
    16441 tcagggaagt attcaaaaag caactgtact tttcccgcaa catcattgcc agcaatagtt
    16501 gaaataaatt caaatgccag atcaatgcca ctggaaattc caccagtagt ccaaacttta
    16561 cctgacttaa cgatacgctt ttcaaccaat tggacatcct caaaagactt taattcaggt
    16621 atagcgcgcc aataagttgt tgcttcatga tgagtcaata gtcctgccgc atgcaataaa
    16681 aatgctccag tacaaacaga aagcaaatag tcactgtttt gagcctgctt tttcaaaaaa
    16741 gaaatcaaac gtggattatt tacttgctct cttctaccaa ccccacctgg aaccagtaaa
    16801 taatcaaatt gtggggaatt gttaaattca caatgaggtt tcaggctaat ttgacttgcg
    16861 caacaaactt cattgctggt ttctgctacc agaaacatat caataggggc atcaagaata
    16921 gttttccagg ttgcaaatac ttcccacggc ccaataacat ccatgggctg gacgttttca
    16981 taaatcaaaa ctcccaattt tttattcgcc gcatcttccc ttttcagcgc gtttttcatg
    17041 caatgccacc ttaatctgaa aaatcccatt ttttatatta gtttttttga taattgacct
    17101 gcatcgcata tatgcctcag attatctatt gctcgcagat tatcagctac ttttatcaga
    17161 gaaaatcgag aaaaatctct aataattaaa agacctcttc atttttacaa ttcgttgata
    17221 agcttcatta atcctttgtt cagaaatctc acccgattga actttttgct cgatcatgtc
    17281 aatgatttct gtcgagtctt gaaatttttc taccaattga ttaccaaata ttagcatatc
    17341 tgctcctgca ttaattgaaa gagtaacagc agtttctaat ccataataat tcgttattgc
    17401 tttcatttgc atatcatcgg tgataacaac cccgtcaaat tttaaatcac gacgcaataa
    17461 tccgttaatg atctggtaag aaaggctggc aggtagccct gtcatatcca gttttctatt
    17521 cacaatatga gctatcatca ccatatcgca atgattgggt tgagataata attgtaaaaa
    17581 agggattaat tcttgctcgc tccaggtgtc acttatatct acaaaaccca aatgtgaatc
    17641 agataatgaa ctgccatgtc caggaaaatg tttataagaa caatgtattt gatgttttat
    17701 aaattgctct gtataaagtt gcgcgtattg agtgacaatt tccggtatcg aagagaaact
    17761 cctgtctttt ttgcctatga taggattatc agggtttata ttcacatcta attcagggaa
    17821 aaaattcaaa ttaaatcctg ttgattttaa agtatttgcc atcaattctg ctgatgcatc
    17881 agctctttcc agagacatgg agccaatgtc ttttgctgaa ggcatttcag ggaatccata
    17941 acggggatgc aatcgattaa cgtttccacc ttcgtaatcg acagaaataa gtaatggtaa
    18001 attatctcta tgatatttac gattggcttt ttgcgtaagt gtttgtaact gattatttaa
    18061 ccgattaact tgttccggac tttctatatt tttatcaaaa gtttgtgact gctgattaaa
    18121 atcaaaaaga ataacaccac caatgttgtt tttctcaatt gcctgggcaa taggtgattt
    18181 ctcgtcaacc actttgccct ggaaccctaa tattaacatt tgcccaattt tatctctcaa
    18241 acccacttgt gtggcacaag ctactgtagc catcaaaaaa agaaaaaaag ataaaataat
    18301 tgacttgatt ttcaatgcta aactcattag aaaatgctta attttctcta ttgcgatatg
    18361 cgccaattat acatatcaag tccaaactaa tcaaatactg tacaataaaa ttaacttaat
    18421 accatccttg atttaaaaat caaggttccc tttataccaa tgccaataaa acagctgtta
    18481 aataatttcc ctctggaaaa gctgccaagg taggatggca gcttgctggt ccgtatacgc
    18541 ctaaaatacg cgcctgcttt cccatcgcaa cagcctgagc gcttaccagc gaacaaaagt
    18601 cctgggatga aagcgctgaa gaacagttgc aagtcattaa taaagatcca ggtttcatat
    18661 atttaaatgc ttctctatgt aaaaatcggt aataattttt agcttgctgt aaatgttttt
    18721 gtgaaggaac cagttttggc ggatcaagaa tgaccacatc atattcgcca gccattttca
    18781 gatattctct ggcatctgcc ttaataaatt caatggtatt taagtgattt aaaacagcat
    18841 tatttttagc ctgctctatt gcctgcgccg agctatctac agccgtaaca tgaagagctc
    18901 ctgctttcgc tgcgtgcaaa gcaaaccctc ccgtaaaagt ataaagatcc aggactcttt
    18961 ttcccctgga cagttttgct atccttttgt gattttctct ttggtcaaga aacaatccag
    19021 ttttttgggt ttgtgcaaat tcaacatgat acaggacacc ttcttccaaa acctgagtgc
    19081 taccctgact tttctctgta tgaatttctt tccagccatc ttgagataac ggcttgtttt
    19141 gaggaagcca aatgatatga ttatgcggga gtaactcttg taatacatga acaataatcg
    19201 atttattcaa ttctacccaa taggcagaac ttgctataac acaatagtca ttaaagcaat
    19261 caatcgttaa accagagagc ccatctgctt cactgttgaa caatcgataa gcattagtct
    19321 gttcatttgg taaatcaaga catcgtctca ccagactagc ttgcttgatt ctatgagtaa
    19381 ttaaagactt gtaatcgaaa ctatttatct gttcattgga gagagcaaga acccttactc
    19441 tatataaaga gtgctcatta taggctccga atcctaaact ctcaccttca gagccaatta
    19501 tctcaaccaa ttcacctgta attagtttgc cagaatattg agctatggct ttggggaaaa
    19561 tccagggatg gcctcttaat acagtgtttt gctttccatt cactaaaata actttcgctt
    19621 tcattctcta tttcccaaaa cctaaaagtc agttaatcta cactatcaag aattatactt
    19681 gcaatggaaa gaaggacaaa tatgcggcaa tttacaaaat acctagccat ttttctattt
    19741 tcatcttcag tgagcgttgc ttatgccggt ccctggttca ccggaccttt attggcaccc
    19801 gcaggccata ccgtccccaa aggccatacc aacctcgaag tttatggcat tgatgtcttt
    19861 actgatgggc aatacaatgc atctggacaa agaatatcca cacccaagtt taaaaccttt
    19921 gccgccaacc caatactcac tcatggcttt actgactggc tggatgtgca gttgaccgtg
    19981 ccttatttat tcaactcgac acgcggagtc catgagaacc atttggctga tgtttcaact
    20041 gcattgggaa tacaaatcct ggagcaaaaa ggttccccta aaagaatcga ttttcgtgtc
    20101 cttttacagg aaacaatacc cactggacgt tatgaccaat tgaatccggc tttactaggt
    20161 actgatgcta ctggtcttgg aagctaccaa acgttagtgg gcttagattt tcaatattta
    20221 ttacaagttt ttaaaaccca ttatctcaga acacgattta ttgcatcacg tcttttttcc
    20281 agtaatgttc atgttgatgg actaagcagt tatggtggtt cattaaatac tcatgggaca
    20341 gtaaaacccg gcattgaaaa taacttagac ttggccttcg aatttactct tacacaaaac
    20401 tgggttgccg taatggaagg ttatattgca actggtaaag ctactcgatt taacggtatt
    20461 ttaaacttta acagcattgg aagcccgccg gtcaatgtca gtgatttgga tgctgccccc
    20521 gtagttattg gcaacggctc atttagagaa gaagcactag ccccggcaat agaatacaac
    20581 ttcaatgaaa acattggagt aattggtgga gtttggtttc ccgtgagtgg agtaaatacc
    20641 tctcattata tgacctatgt actggcaatt aatgcttttt ggtgatagca catgcgttat
    20701 tcctcctatg aaatgaaaaa aaacaggttc acttatggcc ttgcccttgg agctcttggg
    20761 gttgtatttg gtgatatagg tactagccca ctgtatgctt taaaagttac cttatcaggc
    20821 attcctatca atcaattcaa tatattaggc gtattatcgc tcattttttg gtcattaatc
    20881 atcgttgttt cttttaagta cttgatgatt atttttagag ctgacaatga tggggaagga
    20941 ggaattttgg cacttcttgc tctaatgaag cataagagta ccaaatatca acctctattt
    21001 tacatagtcg ccatatttgg agccggtttg ttattaggtg atggtatgct taccccggcc
    21061 atttccgttg tcagtgcggt agaaggtctt ggaactcttt ctgataagct ctacccttac
    21121 gttctcccca ttgcttctgt gatcctcgtt ttgcttttct ctttgcaagc caaaggcaca
    21181 gcaaggatag gctatctttt tgggccactt atacttattt ggtttataac catagccata
    21241 ttaggaatat tgcaaattat agagcatcca gtagttttgc aagcgatcaa cccctaccat
    21301 gccattgcat ttttggttga cgaaggcctg cgaggatatt ttttactagg gggtattttc
    21361 cttgtagtaa caggaggtga agccttattt gcagacatag gtcattttgg taaaaatcca
    21421 atccgattca gctggttttt tatagcgctt ccttgcctgc tattaaatta ttttggccaa
    21481 ggagctaacc tgatagtacg ccccgaagaa ataagcaacc catttttcat gattgcgcca
    21541 ccatggtttt atctgccttt aattattatt gcaactgttg ctacagtgat cgcatctcaa
    21601 gcagtaattt cagcaacttt ttcactaacc aaacaagccg tgttgttagg tttatgcccc
    21661 aaaattccaa ttgtccagac ctccatgctg cattcaggac aaatctatgt tccacaaatc
    21721 aattttattc tatttattgg tactatggca ttttgcctgg ctttcaaaac ctcggataac
    21781 ctggctcatg cttatggaat tgctgtaaac cttgaaatgc ttttagttga tgccatggtt
    21841 gcttatgctg ctgtttcaat atggagatgg tcaacattca atgtgatatt tctctttggt
    21901 ctattccttt tgattgattt agccttttta ggtgctaata ctcataagtt cattacaggt
    21961 ggctgggttc caattgtttt agctttcttt atagccttca ttatgtacag ttggcgctac
    22021 gggttagagt atttaagaga taatttttat atgaacaaag aagatatctc taaaatttta
    22081 aaacaattac aatataaaag tttaaatcaa ctgcctggtg tttcagcgat atttattact
    22141 gatgtttatg ataaaagtgg tggtagtttt ctccattttc tcaaactaaa ccgctcagta
    22201 ccggaaaatg tcttaattgt aaattatatt gttgataaca ttccttatgt tcattacagc
    22261 cagcgctatg aaattgtttg ccttgatgaa aaagtatgta aactagtaat acattatggc
    22321 tttatggaaa caattaatat cccaagaagt ctggaaaaag cttgcaataa aaatatactt
    22381 ccttttaagt tcaatgttga cacagcaaca tttatggttg aaatacccaa tattatggct
    22441 tcgaaagaaa aaagatcact aagtttttat tggcaagaaa aactgtttgc tttcttgatg
    22501 cgcaattact cagcgaattt aaatattgaa ttctataaat taccttacaa tagaactata
    22561 gcaataggta cctattgtat tttatgagtt ttggccaggg tctgttgaca gtttgctctc
    22621 ttgcgtcgaa atggcaacag actctaatca tcgcagtaaa atgctatccg tgataacatc
    22681 ccaaatttat tttaaggact gattatgtgg ttcaataatg cactgatgtt tcaatacaaa
    22741 ctagacggtg aatgtgattt aaaccgttct ttgactgaag aagcgcttaa gccctgcccc
    22801 ccccatgctc gctttactta tggctgggtt cctgcgtttg agcaggaaat agtgcatgaa
    22861 gtagctggta gtacgcttat ttgcctgggt aaagaagagc gaatcctccc tagaggagtc
    22921 attaataaaa tgctcaagga aaaaattcag caaatagaag cagcccaggg tcgaccggta
    22981 aaacgcgcag aaaaggctca aatggctgag gatcttgaat ttgaactatt gcctaagtcc
    23041 ttctgtgttc aaaaaaaact tttagcgatt cttgatagtg tcagtcagag attaattatt
    23101 aatacttcaa gcaacaatca ggcgtctcag ttgacttctt ttttgcgtaa atcaatccct
    23161 ggtattgtga tagaaccatt aaaccttacc gaaaatcttg ccttgcgatt cgcagaatgg
    23221 atccattccc cagcaactct ccctgatgac tttcaattag cctccgattg tttactcttt
    23281 tctcttgaag atgaaaaaaa acgagtccat tgcaaaggct acgaattacc tgccgaagaa
    23341 atcctgactc tattggctca aggtatgggg actgcagaaa tctcattaat ttggaaagag
    23401 cggattcagt tcaccttgac gcaggatttt acttttaaaa aactgaaaag ccttgattat
    23461 ctgatcgatg attttaatga gatcagacaa ttggaagaag agtatcaaag acaagatgcc
    23521 gcgttgacct tattaagtgg ggagttaaga gaacttatca acggattaat gaagatattt
    23581 gttaatcatg cagaaatgaa atcagctcaa attgaaaaag ttgcagaaga ttgcacaata
    23641 taaagtcgta tcattttaac ctgtaaccta tcgagggatc tcctttgcta acagagctcc
    23701 ctctcatctt gacccaagag aaaacgagtg gattgcacat cacttgctga acccaactga
    23761 cagaataaac tctataaatt tctgtactca tcaagaatta attctgacca tattggatgt
    23821 atgataagta gccttgtaaa cagccatagc atcctccgct gccttgttaa gccctaatgc
    23881 tttattggct tcgtacatta ccactaaagc cttttgagcg ctgggagctt gaggatagtt
    23941 tttcactaga taactggctc gttcaatggc agccacatac attttacgtt tgaagtagta
    24001 aagagagaca ttgagttcgt gctgagcaaa catatttcgt agataaatca tacgttgcaa
    24061 agcattggct ttatatttgc tgtcaggaaa tttctggatt aaagtagcaa agtcaaccag
    24121 tgcttgagtt tgtgttccag ggtccctcca ggattcatcc ataggaagca ttttggcaaa
    24181 tacgcctcgt gtttgctgga agttagccaa acctctcata taataggcat aatcaacgtt
    24241 ttttgcgcgt gggtacagat gaataaagcg ctcagccgtt gctgcagcag aaggataatc
    24301 ttcgtcttta taataggcat aaataagctg catttgagag ctttcagtat agtcactaaa
    24361 aggatacata gactccatgg cttccagctg tttggcagca gtagcatatt cctctttttt
    24421 taaagaggtt tgtgccgcag tgtagagctg cttagctgtc atgcctttat atgggttatt
    24481 atcctcatca tcttttcccc atttagtaca cgatgagatt ccaacaacta acccaataag
    24541 aaataacaca tgaattcgtt tcataaagta cacctatcaa ttatatctgc tgtatttata
    24601 agtacagcaa ctggaaaatt tgggacatta tacctaggac cagttaattt gcgaacaacc
    24661 tattattctt ttatttatct atgaaaaaac atttgcaaaa tgatatgcgt tcatatagta
    24721 ttctcgaccg gagagatggc agagcggtcg attgcggcgg tcttgaaaac cgttgaaggg
    24781 caaccttccc agggttcgaa tccctgtctc tccgccagac gacattacca cggtatattt
    24841 tcccgagaca accaatttca ctttagcgtt attctattct tcaagtacaa aaagtaaatg
    24901 aatgacatcc aacccattaa tataaaagac taaagtcatt gtatctatta acttgatact
    24961 gccagatcaa gagttgtttt gatatggagc atcgtatttt ctccatgggt tacttttttt
    25021 gcaaaaattg atgagcggtt ttcactaagc aattgttggg tagtttgccc atcattaacc
    25081 cgtgtggcaa gccaattatc gatggctttt ctatattctg taacatccac aggcaaatca
    25141 gccaattcat ttgagagtac tttaaaccgc tgttgcttcg cttggcgatc tccgaaaccc
    25201 actataagac cagcatgatg aatttcttga tcagccaatt ttcttatctg ttggcaagca
    25261 aaatattctt taaatttcat caaggtttca tgttgatatt tattggaaaa atcgattacc
    25321 catttagcat aataaatcaa actctgcata tggccatgtg gatttctttt tcccaagttt
    25381 ctttccatct ggtttaatac agcgagtaaa tcgctgttac tctggcaatt gcggatgtct
    25441 tgctcgtaat tacctacttc aatatgcgtt ctggaaaaaa agacatgcca gggattttga
    25501 taggctcgca acagatttaa aataagtcgt ttgatattaa agaattcatt cgcctcagca
    25561 atggattctt gcagcaacat gtcaaactcg agtaaagcac gacgattagt aatcagatta
    25621 cgatattgaa atgtttctgt ctttatcggt ttttctttaa aataaagtgg ttgtctttga
    25681 ccaattttgg gaataactac cttgcccaga atcagatcgg caatttgccg attactaagc
    25741 cgttgattat ttttgaaaac catattgctt atcaaaagtt gaatatgatc gtaaaccttt
    25801 tgctcatgct ttggtaaatg atcaactctc cattgagcat cttccctaaa tgcagcaacc
    25861 attctttgat aactgtaata ggtatctttc agacgtaaat ttcctaattt gaaagttgcc
    25921 gtatggttat aaagagtttt aagatgttga ttttcgttta aaaaatcatc aagagataaa
    25981 ctctcatttt tctctttcat tttttgatat tgtgcagcca atattttaaa atgcatcaga
    26041 attgcatccg cagaagattg atgagtagac tccatgtctt cgcgccgctc atctggacca
    26101 ccgggttgaa tagaggtaac gatattgaat ttttcaaaaa tgggctgtga taacatgtca
    26161 tccattaacg cggtatcccc agcaaaataa atatctccat ctaattgatt tgcatgcagc
    26221 acatagccat taaatgctga gcaatgagcg tcggtcagcg atcgacccga ccaatgcctg
    26281 gcaggcactg cagttatcct taataattca ttcccttgga aatctgtaat ctttgcttgt
    26341 tcccaccatt tcaaccctac aacatcggta aatcccatgc ctaaaaagaa tgattcgtca
    26401 ccttccggaa ctaccatctt gggttgctgt tttaataggc gcttcaatgt tgactgactt
    26461 acatgatcat ggtgattgtg agaaatcact actacatcaa ttttgggtaa ttttttactg
    26521 ccagttccat caattaaact cccttccttt gtcatacgcg ggtataggaa aggagccaaa
    26581 tctccttcaa caggatcagt gactacatgc aatggcgaat catgagcagg tatggtcatt
    26641 agatttgatg catggccaat ccagtaatga gtggctgatt gttgtgggct tacccctggt
    26701 tcaatcgctg gatcattggc atagacctcg ctatccaaat agtcatgttc tttataacgt
    26761 gcggtttcaa aaatatgaat acccatttta gccaataacc aattgcataa ggcttttaat
    26821 ctttcccact gagtagagaa gaaaattcgc tgggcttctg agccatgttg atgcgcttta
    26881 tcatcgtctg gagaggtata atgcccagtg caagtatcat attgaatctc aggaagaaga
    26941 tgccgtttca gatcatccct gaattgattc tcaatatgaa aatgttccag tctcttgata
    27001 atcaagtttt tgcgtttcag gtactgacta ttctccaaat taaaattcaa gctttcaatt
    27061 acccggttcg gttgtatatg aaggaaattt tgtaaggtaa taaacaaact agcatataaa
    27121 ctattagtct tatctacttt gtcgcttgaa acttcatcga taattttctt taaataatta
    27181 ctcagggcag tagcatgtat tgattcagat ttgaaaaacc agttccttgt aactcgctca
    27241 ttgtcaaaaa aaaagctgga tattggcgag ttatggtgtg caataaggat attaagatta
    27301 ttaatagctt gttgcatttg agcatcagtt aacatatttt cttaccgtat tatccaatca
    27361 gctgattcgt tagctatgtt ttgccacgag tcataaatgc ctgcttcatt gagttctcaa
    27421 tattttttat gatttcatga catatctttg tttgattacg ctttccactt cctctatacc
    27481 aaaatccaga ttttcctgct tggcagccca tattgaaaat ctattttttg ctactgaagt
    27541 atctcgagca cttttatcct gtagccataa agcatgagcc actttctcag gagtcagcgg
    27601 ttttttctta tttactttga tgcctgataa aaactccggt ttatcaggag ttaggaaatc
    27661 agaaaggttg atttgctcat gtaaattttt ttctaaacga accacatgct ccctatgagc
    27721 actccttaag cattttactt cattagaata atgcgatgtt tctttagcgt actctttctc
    27781 attgacataa atatcgattg accctaccag tcctgttgta atcaaggctg ctccaacagc
    27841 aatgagagct actccgatag gggcaagaac accagtcaaa aataatgccg cactgactcc
    27901 tactattagc cccataaaaa tactaaatcc aaatgaatct ttatttctac tgaaaaaacc
    27961 ctcatttttg ggaggagata accggcttag ctcaacttgt aaatgatgat ttgctttatc
    28021 tttatctata aaatattgtt ttaccagagg attggtttga taatagtttc taatcttctg
    28081 ttcaacactt gcgacttcaa attgacttaa gagcaaaata tttcgattat cctgacgtgt
    28141 tccatcaaat tttatatcat ggaaatcgga ttttattttt tgagccattg ccttaaaaat
    28201 atgggctaac tccgggttct ttctctgggc atcctcaatc attttaatct gtgcataacg
    28261 ctcagaaata ttatttaata ttctgcctat ttcagtagtt tttttattta cagaaggcat
    28321 cttacttaac gataaggaaa tcttgttgga taactcactt agatgctgct cataaattcc
    28381 atctttgaaa aactcaatat ctgcagccat atcagctttt attgaattga tatagtgtct
    28441 taatttttca tcatccaatt gttctgccaa ttcaatgaga atattgaatt gtgcagattt
    28501 tcggtcacga tcgtctatat ttcgattccc tttgatccgt gaagccaatt gtcgaatata
    28561 atccgcattg agtgttccat cttccttaaa aatgcctttt attaaactgg cgactttatc
    28621 attattaatg actctcttta attctctttc aatagcatca ggagtatcca tatcgactgt
    28681 tgattgagct tcagacctta cataatttgg agaagcaaga actgatactt tcctgccctt
    28741 ataattccca acggtatttt ggataactga aataatccag cctttatgat ttccttcact
    28801 atcaccattt ggagcggcaa ccagggaaaa tcctgatggt tgtccagcag aatttttata
    28861 agaaaaacaa attctcgttg tatttcttgt tacttccatg aatgctttag ggtcttcttc
    28921 tgacaaaggt aacgaaaaat gtttggtttg aagaatttct cctattaccg caccagtatt
    28981 gtctccctga gaaaaggatg gataaacacc ttctgatagc gccaaatcac cttgtggatt
    29041 gacagttaac caatcagatt ttaattgtgc aacagtaagt ccatcaaaat ccacgtagca
    29101 ggttctttca agatctttat cgatttgctc aatagtttta gcaacaatat ttttttgtac
    29161 tttggtttgt tgcaatactt cttcaggaat taattcaaca tctttggata aaaatacact
    29221 ttcaggcaat gaaaatattt taggtacttg ttgataagtt aaattgaatt ttttaatacc
    29281 ggacataatg attggctcca atattttctc aatgcacata ttgtaacatt ttattcatca
    29341 agaaaacatt gggatcaatg tgattatatt aacaccatca ttgtctcttt cgaatcaaat
    29401 caaaaacggt atgattaatc agataacagc gaggagcaaa ataaaaaata ttcccgtgcc
    29461 atacttcttt ttacacggga tatcatttaa tttagaaatc agtactgtac tttttataca
    29521 cttcatccgc tgctttcaat gactccaaaa gttgtttcat tttatctaga ggccaactat
    29581 tgggaccatc acttagtgct ttatccggat cagggtgagt ttccataaat aaccctgata
    29641 ttccaacagc tacagctgct cttgcaagag ccggaataaa ttctctctgc cccccggata
    29701 caccattatt gcccccgggc aattgaaccg aatgtgttgc atcataaacc actggacagc
    29761 cggtttcacg catgataacg agggaacgca tatcagaaac caaattattg tagccgaagc
    29821 tcactcctcg ctcacaagcc ataatttgct cattaccttg cgcctttgct ttcgcaataa
    29881 catgtttcat ttcccaggga gcaagaaact gtcccttttt aatattaacc ggcttattca
    29941 tagcagcaac tttctggata aaattggttt gtctgcatag gaaggctggc gtttgtaata
    30001 catctaccac actggagact tcaaataagg gcgtgtcttc atgaacgtca gtcaaaacag
    30061 gaacacctat ttgtgatttg accttttcca gaatcgataa ccctttttca aagccaggtc
    30121 ccctgtaact ggaaatagat gaacggtttg ctttatcaaa agaagactta tagatgaaag
    30181 ggatattcaa ttgaatgcac atttccttta aatatcctgc ggtctctaaa gccagttctt
    30241 cactttcaat aacacaaggg ccggcaatca aaaacaatgg cttatccaag cctgcttcaa
    30301 aaccacataa tctcattttt tgtccttctc ttgatggtga atacgagctg caagtacaaa
    30361 ttccttaaat agaggatgac tatctcttgg atttgatgtg aactcaggat gaaactgaca
    30421 agctaaaaac caggggtggt ctgctaattc tatcatctca accagtgaat tatctgctga
    30481 tcgtcctgaa ataattaagc catgttttac caaactatcc acatacttgt tattgacttc
    30541 atatcgatgt cgatggcgct ctataatttg tggtttgcca taaacttttc ttgccagagt
    30601 gccttcagca agatgacagt attgcccgcc caaacgcatg gttccaccca agtcagtatt
    30661 ttcgtccctg atctgcttgc taccatcggc atccatccat tcactaatta aaccaagcac
    30721 tggatacaat gtttctttat tgaactctgt cgaattggct cctgttaaac cgactacatt
    30781 tctcgcaaac tcaataacag ctgtttgcat acccaaacat atccctaaaa aaggaacttt
    30841 atgctcccga gcatattgga tagccttaat tttgccttcg actcctctct caccaaaacc
    30901 accaggtacc aaaatagcat caatggattc aagtaataaa gcgccatgct tttctatcat
    30961 ctcagcatca aaatagataa tttctacttt tgtttccgta tgaattccag catgcaataa
    31021 agcttcatta atcgacttgt aggcatcatt cagctccgta tatttaccta ccatagcgat
    31081 tttcactgtc atcgtttgaa ctgcttgcat atcaactact cgttgccact cgctcaaatc
    31141 agcctgctta gcttccaggc ttagtttctt aacaacaatt tcgtctaaat gctgcgcatg
    31201 tagaatcata ggtatttgat aaatactatt cgcatcctca agagaaatca cagcttcttt
    31261 ttcaacatta gtgaataatg cgattttagc cctgtctgcc atggataatg gtttttctga
    31321 acggcaaatc aatacatctg gctggatacc aatagaacgc aattccttta ccgagtgttg
    31381 agttggtttg gttttggttt ccccagaagt agcaatataa ggaaccaaag taagatgaat
    31441 aaatattgct cgttgtgaac ctaactcaat acgcatctgc ctaattgctt caagaaaggg
    31501 taatgattca atatcaccaa cagttcctcc tatttcaacc atagccacat cgaatgcatc
    31561 cgcaccaagt ttgatacagc gtttgatttc attggtaata tgaggaataa cctgaactgt
    31621 ccctcctaaa taatcaccac gtctttcttt cttaattaca ttttcgtaaa tttttcccga
    31681 tgtgaaatta ttacgcttgg tcattgttgt tttaacaaag cgctcataat gtcccaaatc
    31741 caaatcggtc tccgccccgt cattagttac gaaaacctcg ccatgctgaa agggactcat
    31801 agtaccagga tcaacgttaa tgtagggatc aagctttatc aacgtaacgc gaagaccacg
    31861 agcttcaaga atagctgcaa gagatgcggc tgctatgcct tttcctaaag aagacacaac
    31921 cccgccagta ataaaaatat attttgtcat tttttgaccc cgctcaaaat gccgaatcca
    31981 ccaatttggt gttcaattta atttgcatgt aaacgggatt caattctatc aaaatgaaga
    32041 tataaataac agaaatttga gcaaaattaa tagaactatt gtaattgttg tttaatagaa
    32101 cgcatagaaa ccgcacaatc tatcgcatct ctccctttat ggccgtgagt tcctcccact
    32161 cttgccaaag cctgctcatc attttcagta gttaatacac caaagataac cggaatatta
    32221 taatctaaca taaccctttg gcatccctgg ctgacctgat cacaaacgta atcataatga
    32281 gaggtttcac cacggataac tgctccaagt gccactatga cttctacttt ttgttttttc
    32341 gctagcaact gtgcaacaaa gggaatctct actgcgccag gaacctcaac taaggtaatg
    32401 tcctccgatt taaaacccag ttcagtcaat ctatctaacg cccctttttt taatgcagag
    32461 gttatccgtt cattaaaagt acttacaact agcgctattg gaaacgtgac accatcacta
    32521 acaattgttt ttatctctct catgtttatc ctaatcaatg gctaataaat gccccagttt
    32581 tatttttttg gtttttaaat aactgtgatt ttctctggag ggctcaattt ccaaaggaat
    32641 gcgttgagta atcttcattc cataacgctc aagagaagcg atttttaaag gattatttgt
    32701 cagcaacctt aatgcttcaa cccctaaata tttcagtatt tggtatgcta cggcataatc
    32761 tctatcgtca gcgggcaatc ctaattccag attagcatcg actgtatcaa acccctgctc
    32821 ttgcaaagca tacgccttta atttgtttgc caaacctatt ccgcggcctt cctgacgcaa
    32881 ataaattaat ataccgcctt cagctccgat ctgggataaa gacaactcca gttgctttcc
    32941 acaatcacat tttctggagc caaaaacatc acctgttatg cactcagaat gaattcgaac
    33001 caaaggcact tgattaccat acaaaggggg tttaaccaag acaaaatgtt cacaggaatc
    33061 tatctgattc tcaaaaacag tcatagtaaa aacaccgttc tcctccagag gaatttgtgt
    33121 agttgcaacg ggatgaatca gattttcatg ctgtactctg tactgaatta aatctttaat
    33181 ggtgactaaa gggatttgat gtttttttga aaaaagagct aattcatcac gtctgctcat
    33241 tgttccatct tcattaatga tttcacaaat cactgcggcc ggggtcaatc cggcaagttt
    33301 taccaaatca acactgcctt ctgtttgacc cggtctttct aaaactcctc tctttctagc
    33361 ccgtaaagga aaaacatgtc caggtgaaat gatatcagat ggcccgcttt ttggatcaat
    33421 agccacttga attgttcgag ctctgtctct tgctgagatg cctgtggaga caccattagc
    33481 tgcttcaatt gaaaccgtaa acgccgttcc atacggcgat ttgttatgcc gagccatcat
    33541 gggcaactca agcttatcta tcaattcttc agccataggc aaacaaatta aaccacaacc
    33601 aagacgcgac ataaaattaa tagcttcggg agtagcgtgc tcagcagcaa tcaccaaatc
    33661 accttcattc tctctatctt catcatccat tagaataatc attttaccgg ctttaagtgt
    33721 cgccacagcc tcttctattg ttgctaatga atgtttcatg acacttcctc ttggttcaat
    33781 ttcccgataa tttgaagctg atgagccaca atccgggtaa gataatcaaa ttcaatattg
    33841 acttgctggc ccactttcaa tgaaccgaga ttagtcattg ataatgtatg tggcaccagc
    33901 atgagtccga tggtttgttc tgatacagta ttaatagtca agctaactcc atctactgta
    33961 atgctacctt ttggtattaa ataatgattg tgttctggga taaaaccatc caaatccatc
    34021 gcgataaatt cattaacagc acaaatggat ttcactttgg ccctggtgtc tacatggccc
    34081 gatacataat gaccaccaaa gcgggttgat gccagcatag ctctttcaag attaaccatg
    34141 tcgccagatg ataattttga caatgtggta acatgcagag tttcaggtga aatatcaaaa
    34201 cacagttcag gaaaatctgc tggcaaaagg gttaaacaaa ccccatttac agcgatactc
    34261 tcaccaggtt tcaaatcctg atagcttgaa gaaacgacta aacgactaga attttctcct
    34321 tgtctattca ttaatacttt gcctgtattt tctatcaatc cggtaaacac tcatcctcct
    34381 gccaatccaa acatgctatc atattgtcac ccatagcttg ccaactcacc gtatgagctt
    34441 tttcaaaaat cccctgttgt tgataagctg aaatcgcttg atgccctaaa ctgccgggaa
    34501 ctaaatacaa ataggttcta tgcaccaatc ccttactatg taacgcagaa aaaatagctc
    34561 cgcccgcttc aacccataca tcatggtatc ccagttgacc aagatgagtt attattgcct
    34621 ccaagttcat caacccattt tgcaaaggca tcgcataatg agtgctatta ggataagtct
    34681 ttgcctgatt tgcttgtggt tgatgataaa tgtggcaatg ttttgctgtc gaaaaaatga
    34741 ttgtgtcatc agacaaactt aaattgcggt caacaatggc aactggcttt gctaccaatc
    34801 cactatctaa tcttgcgttc attcttggat tatccatttg aactgtcttg gcactggtta
    34861 atatgacatc actggcagca cgcatttgat gagtgaactg ttcgcataaa gcattggaca
    34921 aaaccactcg agctccctca gactttccaa ttttaccatc aaaagtttgt gccattttaa
    34981 cagtgactct tggtttatgg gtcatagtcc aataagcata gcttttatag aacacatcaa
    35041 ttgcctttat aggaatatgc ctgacttgta ttccatgttc acgtaatttt tctgatgagt
    35101 tacttttggc aacaatgggg tttgggtcaa aataggaaaa aacaacctcc tctataccat
    35161 gattgataat agcatcaaca caagggggag ttttgcccca atgattacaa ggctccagcg
    35221 tgacataaag cgtaacccct ggcgtttggg gtagaatttg tgataacaat aattgttctg
    35281 catgcggtgt acctgcacct ctatgccatg cctgtgctat aatattacca ttttgcacag
    35341 caaccgcccc aacgctggga ttgggggcac aaaagcctct ccccagtttg gcttgttcaa
    35401 gtgctgcaag cagaaactgt tcgtgcatat attaaccacc ttcttaatgt ggtcgaatga
    35461 taacaaatta ttcaattatt gagtagccta atgattaaat tgaacttaaa ttataagtta
    35521 atcacgaaaa agacattacc taaactattt tactggggtt tggggatcgt cttctttttt
    35581 ttagcaccta caactaatgc cgggagtctt tcaccctata caaccaaaga actggaacaa
    35641 ttagaaagag aatttattca acttattaat cagtcagaca gcgtagagcg caatcctttg
    35701 gccagtcaat acatcaactc cataggaaaa aaactggcac attatggccg tatcaaaaca
    35761 ccttattttt ttattgttaa atccaaagaa atcaacgcat ttgcaggtcc aggagggtac
    35821 ataggcatta atacccaact cattttaacg agcagcaatg aaagtgaact ggcagctgta
    35881 atggctcatg aaattgcgca tgttcgctta catcatcttt acagcattat agaacaccaa
    35941 aaacagatgc gtgttccatt attagcctct attctggcgt ctgcagcctt gggcgtcttg
    36001 aatccaactg tcgggatggg tgctatgatg gcatccgttt ccggattttc ccaggacaac
    36061 atcaatttta cacgctcaaa agaaaaagaa gccgacagaa ttggtataga catgcttatt
    36121 aaggcaggct tcaatcctca aggtatggct gcatttttta ataaaatgca agaaaattcc
    36181 cgttattatt acactgccaa tattccagca atactgcgga ctcaccccct ggatgctgac
    36241 aggatagctg aggctgaaaa tagaaccagc cgtttgccta aaaaaaatta tactgattct
    36301 ttggactatg ccttatttaa agagttaatc agagtttctg tcgcctctaa taataaacaa
    36361 attcttgatt attatgagca tcattgccat aaaaaaaatc ctgccactgc ttgccagtat
    36421 ggatacgtac ttgccttgtt aaataataac aattttcaaa aagcagctga aaaattaaac
    36481 cctttactgt tacaggataa tagtaatctc aattttcaaa ttgctatggc tcaggctgaa
    36541 ttaggtttat cacaatatca atcagcactc tcccgtcttg ctgaattaca aaataatttt
    36601 cctgacaatt atgctgctct aatggcatac gcccaaggat tgttaaccgc aaaccaacct
    36661 caaaaagcta cagccgtact tcttaagggt agtaggcaat acaaagagga cttaccgctt
    36721 tgccgagaat tggcaagggc tcaagctgaa tcccaccaga aaagttatgc ctattttact
    36781 caagcgcaat gcttactact tgaggggcaa aaaagaggtg ctattgctca attaaaagtg
    36841 gcgcagaaac tggccaaaaa ggatgcttat ttatctgcaa gaatttcagc aaaaattgat
    36901 gaaataaaat ttatggttga aaactaatct aaatatagat taattaaaat aaaatgatct
    36961 catttatcat tcgctaagag tgtaagggtt taattttgct ttgttcaggc ccaatataat
    37021 taattaatgc tcgcaagcat ctcacttctg ataggtctta aatggaaaca aaatagaaca
    37081 tatttatcaa aaagaaatta gatggcaaaa aggtctcttc ataaataatt ttcgatattt
    37141 tcagtagaca agatcatcta aaaagccagg tattaatttg ctttcacttt ggatagggag
    37201 ttgccatgaa tgaaattcaa gaattcttgc gtcaaaaact gaattcgccg ttacctattt
    37261 cacgacctca attggcaaag ctaccaatgg gtactatgga gttcgttact tatggtaatg
    37321 gaaagcccgt actgattatt catggtggag ggggtggtta tgatcaggca gtgcttttat
    37381 tcaaacgcta tatccccgat ggttaccaaa tgatttgtcc ttctcgtccc ggttatttgg
    37441 gaacacccat tgatactgga caaacagcag aagaacaggc agatgccctg gccggcttac
    37501 tggattattt gaatattccc ggcgcagtta ttgtagccat atcagctggc ggtctttctc
    37561 tgtatccttt tgccattcgc cacccgaaaa aaactaaagc aatcattgca atagatgcta
    37621 tagctggaga atatttaatg cctgaacaat ccaacaaatt ggctcaagcc ctttttatga
    37681 ctgattttgg tttatgggtc gctaaaaaaa gcatgattta tttccccaag tcatttatca
    37741 agaatttaat taaactggaa ggttacctgg atccagtacg tgtagatgac cgggtgaaag
    37801 agatcttaaa ttgtcgggag cgcctggaaa tcattgctga actaatgaat agcatggcgg
    37861 actataaggc ccgtaaaaca gggaccgtga atgacatggc agagggtgta aaaaccagct
    37921 ggtatgattt ttctaaaatt aactgccctg cactaataat tcatgggaca catgacgctg
    37981 atgtaaaatt ttacaatggt gtatttgctt atgagcgatt agcatcgaaa gaaaaagagc
    38041 gcttctggat tgaatacggc actcattttg gttttttctt tgctcatcaa gcgcgtaagg
    38101 cgcaacaaaa atttcgggaa tttgtaataa agcatagtta aacgggtttg atgttgaaaa
    38161 tccaggaaat gggagtatag gtcggtgccc tcatgcttaa atattctcta acgatcagat
    38221 ttgagtttag gttagatatg ctaggtggta ggagtttata cgcgggaatt actgatatcc
    38281 agatgggaaa ttataaaata tcattataat ttcccaaaca attcatctaa ttaaattact
    38341 ttacagcaac ttctttccac caactggttt tggtttctgc aacccagtcg gcatttctta
    38401 aatttttaac aaacaaagca gtttcctcag ggcttgagtg cttggagtca agttttatac
    38461 accagtccca gtctccagag gtagaccaaa gactttgaac accattcatc tttgctattg
    38521 cagcagaatc tgaccaggca tttttagttt ttaaaaaaac aacactgttc catgagttca
    38581 ttgtttcttc cttaatttta atcaattatg ttttttcgca ttgaacataa attcattgcg
    38641 ttatataaaa ttagactatg aattatcaga tttcaaactc gaatgaaaaa caattctgat
    38701 attagacctt cttcctaagc taattatttt gtttcacggc aaggcgagaa cgttttgagg
    38761 aacagagttt acatacacag gaattatgaa aaactccaat gagggagttt agataaacta
    38821 aatgactgaa ttaaaaacat tttttaacat agagattggg ggttttcata agtcctgata
    38881 cagtaaatga gcaactcaga actgttttca gggcgacaat aaaacgaaaa aggctggtca
    38941 ggcaagaggt ctatgttaac ctttgaaaac cttcaaagcg caacatctaa tcaaggccca
    39001 gtttttttaa tcgataacgg agagttcgaa agctgacccc cagtagttgt gcagcagcgg
    39061 ttctgttcca ttttgttttc tcaagcgcat caagaattaa ctctttttcc tgatcctgaa
    39121 ggtaaccatt taagttagtt gacgtggagg gtacggcaac agctggggtt attgttgttc
    39181 ttggcaaatg taaatcggat ggttctataa cctctccttc acacatagcc atggcacgct
    39241 cgaggatatt ctccagttct ctgacatttc ctggaaaatg ataacgactc aatatttcaa
    39301 aacaggatgg atttattttc accaggtttc tgttttgatt tttagataac tttcttaata
    39361 tcgcctctgt caggcaagga atatctgaaa gcctttccat taaagttggg acacgcaatt
    39421 caatcacatt aattcgataa tacaaatctt gacgaaacgc ccctgcatta acttcctcaa
    39481 tcaaattttt atgacttgca cttaggatgc gaacatccac aggaacttca tataactcac
    39541 ctatcggctt aattgccttc tcctgaatcg cacgtagcaa tttaacctgc atagcgagag
    39601 gaagttcggc gatttcatca agaaacaaag tgccaccatg agccgcaaca aacaaccctg
    39661 ttttatcaga aattgctcct gtaaagctcc cttttttatg gccaaaaaac tctgattcca
    39721 tcaactcacc aggtatagca ccacagttta caggaataaa tggcttatcc tttctgggac
    39781 cattaacatg gatcaactga gcgaccaatt cttttccaac acccgaagct ccttgaataa
    39841 aaactggtgc ctgactgcga gctaatttat gaatgttttc acgaagttgt tgcattacca
    39901 aactttcacc aaccagtaga tcttcatcag gattctcggt aggcgggttt tgcatggata
    39961 atgccgtctt aacgagatct ctaagcattt ttaagtctat aggttttgat acgtaatcaa
    40021 atgcacccgc ctttaaagtg ttgaccgcac cctcgacatt tccataggca gtaatgacgg
    40081 caactggcat ctgcggtttg tatttctgaa tgaatttaac aatttctata ccatctccat
    40141 caggtaaacg catatcggtt aaaaccaaag aataataatt ttgttttata tgttctatgc
    40201 cttgcttaaa gtcagacact gcatcgcaag ttaaacccat gcgtgataaa gtaagagtta
    40261 ataactctct tatatctggt tcatcatcta taacaagcac tttacttttg gtcatattaa
    40321 aagttcatcc gatggattaa cagtgatcgc aaaacaacat cctttatttg attttaccaa
    40381 atttaatctg gcttgattaa tttcacacaa atctctggca ataaataacc ccatccctgt
    40441 accacctcgc aaggtggtaa aaaatggttc aaatatattg tctctgtgct cagagggtat
    40501 accaggtcca gaatcacata cagtcaaagt gattttattc gaagaacatt tggcagcaat
    40561 gacgatattc acattaccct catgatcccg cccatgttga atagcatttt cacacaagat
    40621 tactaaaatc tgttccaatt gacttttatc aaaaacaact gataacggct tatttacagg
    40681 tagttttatc gtaagatcac agggattatt gtgagtaaaa tcctgcttaa aatgttctaa
    40741 aaatgttgta agttcattaa cctgcggttg agatttctca cgccttgaga gctgcaatac
    40801 atttttaatc accctgttca ttcgctcaca attgttcatg attaattgtt ttaaacgagt
    40861 gttctcttca tttaaatcct cattatctcc aaacaattga atagcatgag caattgcccc
    40921 caggggatta cgtaactcat gagcaatact tgccgaaaaa cgccctaaag ccgctaattt
    40981 gagttgttgt gcttgttgtg caatataagt catgtcttcc agaataatga gtacttcaga
    41041 cttgtgacca acagaagtag aaaaaaaatg tactcttaga taaggatctt caataatagt
    41101 ttgagccgta cgttcattat tcgttgtttt taaaagaaaa tcatcaaatt tggatacaag
    41161 tgaatcagat agttggtcca atttttgaat tgcatgatgt ttactcagat taaaaaattc
    41221 acgggcagca gtattcatca atcgaattcg ctttccctct gcaacataaa taatgcctga
    41281 atgcaatcgt tcgacaatat attcattgag cctttgcatt cctgccaact catagctacg
    41341 gtgtctggcc agactctcag acattctgac ccaattggat aaataccatg cggtgatagc
    41401 cgttgcaaaa aaaccagccc catagattcc actataaaaa aaagtgctta agtctttttg
    41461 attataaata aaaaattgca aaacattgcc acatagcaat aaacaactgg ctagtgatgc
    41521 aaaatagatg gccaagcgcc caggaactaa aatacttaaa gcagcaatag taacatttag
    41581 taatatgcca taaccggatt ccaaattacc aatgatgcct agcatgctgg aaatggctat
    41641 gacatcaatc gtacccgata aaaacacctg cttatcaaaa cttaaaattt ttctatgcca
    41701 aaaataaaga aaaatcaaag caaatactaa ataggcaaaa gcgatacaga gaaataataa
    41761 gggataatat ttattgatat aactgaaata ataaatgcct aaaaatagaa aaatacttac
    41821 tattctgtaa atattgtaaa ccaacaatat tcgccattgt ctcattttcg gattgttgat
    41881 ttggcgagca gcagacatcc ttttctaatc cattagatta ttcagcgtat tttaattata
    41941 taaccagact tgagcatttt gcacaataca tcaactaaga cccacaccaa tgttcactaa
    42001 tatttccaca atttattttt attccatatg gtcagcgcat cttgagaacc gctgtcctta
    42061 gcttttccaa tccaatactt tgctttagcg aaatctttat taacacccat gccatcaaga
    42121 tacataatac ccagattaat ttgtgccaaa tccaaacctt gttcagctgc cttttgatac
    42181 caatacatgg ccttcttaaa atctattttt actccttcac cacgtatata cataaccgcc
    42241 aaattgtttt gcgccaaatc caatcctttt tcagcagctt tttggtacca tttaatggcc
    42301 agtttattgt ctttctttat ccccaaccct ttcatataca taatcgccag attatgttgc
    42361 gctaaaggta atccttgttc tgccgctctc ttgtaccaca agactgcttc gtgataatta
    42421 actgacaatc cattgccttt catatacatt aaacccaagt tattttgggc ttcggcaaat
    42481 ccttgatctg cggctttttt gaaccataaa taagcctttt tgtaatcttg cagctcgcca
    42541 tcatttgaat agagaacacc taaattattt tgagccagag taaatccatt ctctgcggct
    42601 ttttcgtacc agtaaatagc ctttttcata tccttttctg taccaattcc ttgttcataa
    42661 atatacccaa gattattttg agctttttcc ccaccttgtg ccgctgcttt ttcataccaa
    42721 tatattgctt tcttgccatc ttgaatggaa ggatcaccag tcatatacaa tacaccaagg
    42781 ttagtttgag caatcagtga accttcattt gctgctctgg tataccaact aatagctgct
    42841 tttaaatctc ttggaacctc ttcgcctcgc tcataaagaa caccaagatt attttgagca
    42901 tcaagatctc cttgctctgc tgctttgata taccagtgaa tcgccttctt gatatctttt
    42961 ggtataccat tgccatacat gtacatcact gcaaggttat attgtccgtc tgaatttccg
    43021 ccttctgcag ccttttgata ccagataaaa gcctggccca gatctttttt aacacccagg
    43081 ccattttcat ataggatccc tatattattt tgaccaattg ccaaaccctg ttccgaagcc
    43141 ttcttaaacc aatgcattgc ttgctgactg ttttggggca ccccttctcc tttaagatac
    43201 aatacaccga gattattttg tgcctcagct tgtccttgat ctgcagcttt ctgataccaa
    43261 taaatcgctt tctttaaatc tttatttact ccatcgccct tttcatattt gagccctaat
    43321 tcaaactgtg cttgagcatc accctgttcc gccttatcct ctgtattgaa ttgtttttgt
    43381 gaactacgat aggtatcaac atccagcgct gataaagggg aagttagaag taataagaaa
    43441 aatcctgcaa caaccaattg tttcatcata cctccctgca ataattgttc aattatagca
    43501 caattgatga tgaataaaca ttgacgctct ggtgccacac aagcaaagtg catctattag
    43561 gagtaagatg taatagggaa atgttgctat cccgcattac agacttctcg taatacagga
    43621 tagcttgcaa ttagaaagta attttcatct catcatcaga ttcagagagg ttttctaatt
    43681 ttaaatcaaa ggattgtacc caatccaagg tcttgttaat cagttcaaca cagcttgagt
    43741 gctctccata acgccctgca attctcggtt tatattgaga aaaatcttta tataccgatt
    43801 ggagagtagc aatcatatag ccttcaacat attctttgtc atcaaacatg ggagattgac
    43861 gatttaaatt atcgacaata tttttggccg cttgagcata gtttacaagg agttgatagt
    43921 aattgggctg ctgttcacta aaaaatcctt tggcgccaaa taattgcctt atctgagcat
    43981 cagcagacac tgcaatttta ttccacattg tattataacg ttcttcaaag tcagaatctt
    44041 gaaatctcat attaattcac catgtacaaa aacatggcat ataatactaa aaatttatta
    44101 tgtgaagatt aagagcaacc taatttaacc attactcttc ttgggcccca atgaccaact
    44161 tctcccttaa atactcctgc aatttgcgta cgacaattta agcaatgtcc agtatcatcc
    44221 acccgataat ccaatattcg ataccaatcc cttacaatca cgggtaattt acaatgagga
    44281 caataagtgc tgctgccggt tgtatcgtga acatttcccg tatatacata atgtaaacct
    44341 gtcttgattg cgatttctct ggctctttgt aaagttgaaa atggagtcgg aactttatcc
    44401 atcattttcc aatctggatg aaacgcagtg aaatgtaaag gaacatcagg ccccaggtta
    44461 gtcataatcc attcagatat cctctcaagc tcggcatccg aatcattttc ttctggaatc
    44521 actaaattag taatttcaaa ccatacactg gtttccttct tcaaataaat gagcgtatcc
    44581 agaacaggtt gtaaatgaga accagtaatt ttgtaataaa aatcttcact aaaggatttt
    44641 aaatcaatat tagcagcgtc catgaatgaa taaagctcct tacatggctc aggattaata
    44701 taccctgcag aaaccgctac cgaatgaatg ccaagcttat gacattcctt tgcgacgtca
    44761 atggcatatt ccataaaaat caccggatca ttataggtat atgctacact gaggcagttc
    44821 atattttggg cagcccttgc aatatcttct ggcatggcag tatctgccaa tgtatcaata
    44881 tctctggatt tgctaatatc ccagttttgg caaaacttac aggccagatt acatcccgca
    44941 gttccgaaag ataagacagg gctaccgggc aaaaagtgat tcaatggttt tttttcaatg
    45001 ggatctatgc aataaccgct agagcggcca taggtagtta attttatttg attatccagg
    45061 tttgcgcgaa caaaacataa ccctctttgc ccttcacgca atttacaatt acgaggacat
    45121 acatcacatt gtatgcgccc atcttccagc ttatgccagt atttggttgg aatagtatca
    45181 aacatcaaat agcccacaat caactcttag tctatattta taagtatagt atgttcagtt
    45241 gttccggagt gttgttgtga gtgtccgtca accagccgta gctgggtatt tttatcccga
    45301 cgatccattg actttaaagc aaaccgtatt gaatttcctg gaccaagcgc caattcataa
    45361 gcctgctcca aaagcaatac tggttccgca tgctggttat gtctactcag gatctgtcgc
    45421 tgcttctgct tatgcttcgt tgcgagataa gaaaaattcc atcaataaaa ttataatatt
    45481 aggccctgct cacagattat atttcaaagg catagcctat gatcctgtcg ataaattcgc
    45541 tacaccatta ggagaaattc aacaggataa agagttacta actcaaatta tagatctgcc
    45601 ttatgtttat tcacttcctg aggctcatca aaatgaacat tgtctggaag tacaattacc
    45661 tttctgccaa atgatattct ccaaatttaa aatacttcct ctggttgttg gagaaacgaa
    45721 tccccaggat gtagctcgtc taatagcgcg agtatggggt ggagatgata ctctcctgat
    45781 tatcagctct gatttaagcc attatctgcc ctatcacata gcccaaagag aagataaaaa
    45841 aacttgcctt tccatagaca ctttaaatgg tgaagaaata ctgcatgatg ctgcttgtgg
    45901 ttattttcca ctgcgcggtt tcctgtattt cgcacgccaa caccatattt atagccgtct
    45961 gctcgattta cgcaactcag gcgatacagc tggggataaa gaaagagtag tggggtatgc
    46021 ggcctatcac ttctaccaaa aacttaattt ttcagaacat tgcgcagatg aattgaaata
    46081 catcgcgaaa gaaacaatac gattacaaac agaggaaaat aaagcattgg caattaatta
    46141 caatgactat aatcaattat tacagatacg gatccccact tttattactt taaagaaaaa
    46201 tggtttgcta agagggtgca tgggaagcct tatagctaaa gagcaattag cagataacgt
    46261 catttacaat tccatacggg cagcaactgc tgacccacgc ttcccacaaa taagaccatc
    46321 tgaactcaag gaattgtctc taactatctc attaataaaa ccattatctc cactatattt
    46381 tgacagtgaa gaagaactca aatcacaatt gcaaattggc atagatggtt tggtattaat
    46441 ttatggctcc tatcaagcta ct
//
LOCUS       DBNCWK010000013        45754 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.013, whole genome shotgun sequence.
ACCESSION   DBNCWK010000013 DBNCWK010000000
VERSION     DBNCWK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 45754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45754
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.013"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            92..226
                     /gene="rpmH"
                     /locus_tag="AC06IO_000607"
     CDS             92..226
                     /gene="rpmH"
                     /locus_tag="AC06IO_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIO9873821.1"
                     /translation="MKRTFQPSNLKRKRDHGFRLRMSTRAGRLVIKRRRAKGRKRLSA
                     "
     gene            227..571
                     /gene="rnpA"
                     /locus_tag="AC06IO_000608"
     CDS             227..571
                     /gene="rnpA"
                     /locus_tag="AC06IO_000608"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIO9873822.1"
                     /translation="MFAFKKAQRLLKKNDFDFVFESAKKITTDDFIFLFRENKLGYAR
                     LGLALSKKMIAKAHDRNRIKRLLRESFRHTNLPAVDIIILARPGLAKKTNLGINTKLN
                     KTWEKLTSCYGK"
     gene            538..783
                     /gene="yidD"
                     /locus_tag="AC06IO_000609"
     CDS             538..783
                     /gene="yidD"
                     /locus_tag="AC06IO_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIO9873823.1"
                     /translation="MGKINLMLRQIVCLPIKMYQYFISPLITPCCRYYPSCSEYADSA
                     IKHYGVIKGLLMALNRLSRCHPWSKGGYDPLFPNDKN"
     gene            793..2463
                     /gene="yidC"
                     /locus_tag="AC06IO_000610"
     CDS             793..2463
                     /gene="yidC"
                     /locus_tag="AC06IO_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIO9873824.1"
                     /translation="MDIRRIVLYMALALIGLSLWNAWQIDYPAKQPVEEKTASQLTSD
                     GHLLPQIIPSNAEQPVTLKAEEKASSGKQLIQVKTDVLDVDIDLKNGDIVKGLLLDYP
                     LSVEDKNKPFPLLQNQASQRYVANSSLFVLDGQTPQSLDFDFTSEKEYYELKPDQNQL
                     IVTLNGKSEDGLDVKKEFVFTKGSYLIEVNYKIANIGNSLWKGYFNTQLLRSSPKEDK
                     SSIFHIGSYTGASFSNPGKNRYQKVSFSDMSKSNLDVDAKGGWIAMQQHYFLSAWVPN
                     ADSENKFYTLATDKDYTIGAVSQPITVKPKEDKIVGSKLYIGPEITSVLKGISPSLDL
                     TVDYGILWFLSSLLFSLMKAIYTVVGNWGWSIVLVTVLIKLAFYRLSATSYKSMASMR
                     KLQPKLQALRERYGDDKAKISQATMELYKQEKVNPLGGCLPILIQIPVFIALYWVLLE
                     SVELRQAPFIFWINDLASADPYHVLPLIMGATMLIQQKLNPAPADPMQAKVMMFLPIL
                     FTGLFWNFPSGLVLYWIVNNTLSILQQWYITRKYSDEKPAKKVVATAK"
     gene            2464..3804
                     /gene="mnmE"
                     /locus_tag="AC06IO_000611"
     CDS             2464..3804
                     /gene="mnmE"
                     /locus_tag="AC06IO_000611"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIO9873825.1"
                     /translation="MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPR
                     LATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIEIQAHGSPVVLDLLIKESIAAGA
                     RLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
                     ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQRIIGRLEEIRSQANQGVLLREGLSL
                     VIAGRPNAGKSTLINNLAGRDVAIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDS
                     DDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLTLPNKIPIITVYNKI
                     DTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAK
                     ALLLTGQSQLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK"
     gene            4099..5937
                     /locus_tag="AC06IO_000612"
     CDS             4099..5937
                     /locus_tag="AC06IO_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg3000 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873826.1"
                     /translation="MVYSKQSAAFRRDKQQFFAQIPKDSKLDTKIVTIDDRVNTIDYT
                     FLLHHKQDLEAEFTQMFTVLQQQDDENKEIFWLYCYYCASLLEAFHKAYSQNSKQAEY
                     AKIKQKIKDHLNKVNVQEEESGFIQSLYDSFLGGFRNLISSPLHASKIRDYVAYSNLC
                     RIYWTFCRLTLTNGLSVARDLKIIEKLDAILGTHTDVDKIISTLQAPTGVITYFSVGF
                     FLMRLIIDGGMLLRHTFFPTELEKGAENGCEIHQMDYLPGPASLEAYRKSYILVHEDN
                     QEVSLYYIPRSGKAEKLSIKENNKLKDALLNKMNKDTSSLRLSSTEVEEIITHQTGHV
                     PEVTTAWDRFKHEFYKRHCNFANDFVWATINLLTNYNYLFNISGPAAGMITSVFLGFD
                     VCMALYKCNLAKQEYLIKKAQYLQEIEDYNNPEKFKKMTPEQRLLHIEMLNKQLRELE
                     INYKTQEATFYFVAAAAALLMMGFTASMLVSPPVLVVGCFFACTLAVAMYLSTGSYSK
                     YEEKSLYLEEAQLTGKNLPVALKEYEAARNDFIFTMVKNTVMPIVLITTFAICWPAAI
                     VLTAMYIGYEIFHAYDQHSEAKAAKQLALAAPSEEKDGYMALATPN"
     gene            complement(5934..6734)
                     /gene="legP"
                     /locus_tag="AC06IO_000613"
     CDS             complement(5934..6734)
                     /gene="legP"
                     /locus_tag="AC06IO_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegP"
                     /protein_id="HIO9873827.1"
                     /translation="MKTKSWIASLFYSSLCFSSSYAINLHQITVADPVMGSRTITYEQ
                     VGEFAVVEGDILIGKAENLKNQGAVITPKVGGSRWPNGVIPYEIAENLPFINKLAILQ
                     AIDHWQKNTNLEFVELTSKNRYDYRDYISFIPAEGTTCSSYVGRQGGKQEINLAPRCT
                     TMNTVHEIGHALGMWHEQSRSDRNQYIRIVWENIDEEHKFNFDQHLTDGKDFGEYDYG
                     SIMHYGRFAFSKNGQPTIIPLVDGVEIGQRTHLSEKDIAAVKAMYPET"
     gene            7223..9388
                     /locus_tag="AC06IO_000614"
     CDS             7223..9388
                     /locus_tag="AC06IO_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIO9873828.1"
                     /translation="MTRSQLILAILAGLVISIDNMISIVAFSSLIYQGALEIYIPLVI
                     NILAISVIIISASYLFMSSPPYAIAQLQDEAAILYSAMGLVIIKSMPSSSTNEAIFIT
                     ILFMLGVTTCLTGLSFYFVGKFRFGNIIRYLPYPVICGFMAATGWLVLSGTFALLTGK
                     ALHQNWLVFFHKEEILLWAPGFIAGIVVYIFKEKLGYRYALQLMFISLVILFYALFYI
                     FNLEYLVDPTEGYVLGPFNQSHIDSLMKPEIWQMIHYPFSMTLLNYIGLVILVSFIAL
                     LLNASSYELIVNKQLDLNKELRIAGIGNVFSGLLGGMVGYLSLSASTVAKEYGGARII
                     GVMAFVPMLLILCFGAVVVECFPKMAIGCYLFYIAFCFLSEWLINTWRLLNLSEYLIV
                     LLILVIAIVFNMITAVAIGTVISIFIFAFRYSKINPVKYLFSGADYNSSFERNPISQS
                     ILSTQGDEIQFLKLQGFLFFGSIYRLLQLIKNIQKARYIILDFELVYNIDSSRIILMK
                     NLKLHAEKNNISFAICSLKPIVYHELMKTNLLQSKTNWLKVFTDQESALEWCEDQVIE
                     KYTENINIEEVTLEKQLLYLGFSSELVAWISEKASIKPYNSDDIICHEGEESNSVYFI
                     HRGRVTAFVGDKRVLVVGSGNVLGEIAMYTHKKRTATLKTNEKSIVYEIDLKSIQELS
                     KNKPLLLVQFHACMAKILANRLSVLNKRIKVFDSTAILQ"
     gene            complement(9439..10506)
                     /locus_tag="AC06IO_000615"
     CDS             complement(9439..10506)
                     /locus_tag="AC06IO_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HIO9873829.1"
                     /translation="MDDLKKYSFLLVYLLIPLPFVSLYLGGWYSFLPFFIVFAFIPLI
                     DAFLHDSVNPTKEQEQELLKDKFFKYITCAYVPIQITIIAYGVYIVSTQNLIWNEWLG
                     FTMSLGLISGGVGINFAHEMMHKNSKLQQLMSKILLVTVCYGHFFIEHVKGHHVKVAT
                     PDDPATARLGENLYQFLPKTLLGSFQSALLLEEKRLSHKNYSIWSIHNNFWWILTVPF
                     MICIVTFIFGGLIALSFFLLQSFIAILTLEIVNYIEHYGLERKMLSNGQYEKVNPNHS
                     WNANHWLSNQLLIHLQRHSDHHAHGARPYQILRHMENSPQLPSGYLGMIIIALIPPLW
                     HYIMDKRVVAYRIQLRQLEQL"
     gene            complement(10636..11487)
                     /gene="rsmI"
                     /locus_tag="AC06IO_000616"
     CDS             complement(10636..11487)
                     /gene="rsmI"
                     /locus_tag="AC06IO_000616"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIO9873830.1"
                     /translation="MTNTLATGLGTLYIVATPIGNREDISFRALNTLKSVDLILAEDT
                     RHSMQLLTSLGIKNNLTSLHAHNEANKSNEIIELLLHGKSIALISDAGTPLISDPGFP
                     LVKQARQHHIPVVPVPGACALIAALSAAGVPCDSFAFLGFLPAKQSARKDALESSRSV
                     PYTTVFYESTHRIIDCLNDIAEIYGQDYELVLAKEITKTFERFVSGKIKEIKDWLLSE
                     PGHIKGEFVLIFPPRSTNKDLNSHEELLKILLEELPLKQAVAIACRLTNANKNQLYEE
                     ALKLKGS"
     gene            11527..13338
                     /locus_tag="AC06IO_000617"
     CDS             11527..13338
                     /locus_tag="AC06IO_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIO9873831.1"
                     /translation="MLIKQLKLRMFLVLVSLFLLCQCTKTVNSTEPVVTVNKKLKTPY
                     SLPTAAYLAMAKSQQGHEKQSALISAAGRLITDGQWKQGTAILAQTGELTVEQANEKN
                     LLLAKVDIMRDRPRDALAKLARIKEPERLSLYNQIQFHEQLAKSFRSLGSYSESVSER
                     IKLESLLPDEESQVNNRRALWLTLTSLPQEELNTMALETVNKSEMQGWLQLAVISRKY
                     RNNSKSLLAALDQWQIHFSNHPANQILPNPLDSISGKLLAPPKQVALLLPLSGPLSGP
                     GNAIREGFMAAYKANRGDESTKIKVYDTSKGDITNTYRQAISDGAEYVVGPLTKTQVA
                     TIASMEHPVPTLLLNDTDTSTQSNSYSLGLSPVNEAIQVAIKARSKGYKKALIIAPNN
                     AWGNEVTKAFINQWNEDGGHIVDSLRYEAKQDLNKSMKDFLQITNSQEREKKLKQVLG
                     YNFQSTTSRRQDFDMIFLLAYPSKARQIMPLLKYYYAGDAPVYATSSVYGGSANALKD
                     KDLDGIIFCDMPWVFSHQMGTKNWPEQFNSYNRLYALGVDSYTLATQLNQLILFPADG
                     SNDSTGILYLKPTQQVARVLEWGQFKQGLAHSLGDTV"
     gene            13360..13716
                     /locus_tag="AC06IO_000618"
     CDS             13360..13716
                     /locus_tag="AC06IO_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIO9873832.1"
                     /translation="MTQEKGKFAEQLALNYLKENGLALVMQNYHCRLGEIDLIMREGS
                     YLVFVEVRSRSNMNFGGGLASITYEKKQKIIKATSHYMIKYRIQDKFPIRFDVISIDG
                     KSNKITWLKNAFDAGC"
     gene            13743..14342
                     /locus_tag="AC06IO_000619"
     CDS             13743..14342
                     /locus_tag="AC06IO_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIO9873833.1"
                     /translation="MIQLEERVRHLFGVTIEAKISAADVLSGSIAKAGKLLVNCLLND
                     GKIFICASGGSSANCMHFSSSMLNHFEVERPSLPVINLCADASCLSSFADEHHFGQVF
                     ARQIQALGQEQDVLLLLTTSGNSDSMLQALHAASERGMDTIALSGRDGGVLANHMGPE
                     DIEIRVASDSTALIRELHLFILHCFCDLIEQSLFGQVLG"
     gene            14346..14918
                     /locus_tag="AC06IO_000620"
     CDS             14346..14918
                     /locus_tag="AC06IO_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIO9873834.1"
                     /translation="MNINLKCKSIIVMMIGALLTGCVAVVVAGAAAGMVYDRRSVTTM
                     EADARLFHVIHKTIIKDPRFRHSRILVSSFNRVVLLVGQTPAASLRVLAERIAQNAPG
                     VKRVYDEITVDNPIPLTQRTKDSWITSQVRSNMLTKKGLESGSIRIVTENGVVYLMGI
                     VSDEQALLAVDVARRVAGVRKVVKIFQYIR"
     gene            14920..15507
                     /locus_tag="AC06IO_000621"
     CDS             14920..15507
                     /locus_tag="AC06IO_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIO9873835.1"
                     /translation="MFKHGCFILIVFFLASCLSGCLGALWTGATLAYDRHNVYKKLDD
                     YNLIKALNDVLAVNRTFKNSETVLDIAVFNRDILIAGHVPTQELYDELQLRLSKVKGY
                     RRLFNRVIINKMPSNSIQDSWITTKIRSQIFADSSIDPNAFKVVTSDRVVYLMGDVQA
                     EQAEKVIKIARYTGGVQKVIKLMRYYTYQTSTNMA"
     gene            complement(15577..15822)
                     /locus_tag="AC06IO_000622"
     CDS             complement(15577..15822)
                     /locus_tag="AC06IO_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873836.1"
                     /translation="MSEKIQHTAARLQEKMSNRYSDGLSHKHNKNHLSYRQHCRSHPC
                     NQTNNKDEENIVNGSTGLLREFNLRKIRKRLDKGRFH"
     gene            15976..16371
                     /locus_tag="AC06IO_000623"
     CDS             15976..16371
                     /locus_tag="AC06IO_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIO9873837.1"
                     /translation="MNKQLNKRGITRLWLAQLGVTVILAGLCAILFGVNAASSALLGG
                     LVCIIPNAYFAIKLFKHQGARAARQIVNSFYKGEALKIILSMFLFALVFLLCRITPAA
                     FFASYILVLMTHWFAPWIIVNKQNRPKSD"
     gene            16373..17200
                     /gene="atpB"
                     /locus_tag="AC06IO_000624"
     CDS             16373..17200
                     /gene="atpB"
                     /locus_tag="AC06IO_000624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIO9873838.1"
                     /translation="MVSSTNYIKHHLTYLTYNVNDMTFGSSGGFWTLNLDTIFFSIAL
                     GLIALAIMYLAARKVTTGVPGKLQNFAELMLEFADNQVKDCFHGKNNLIGPLALTIFM
                     WVFLMNFMDIVPVDILPVLAQLVGIHYLKVVPTNDLNLTFGLSLSVFFLIVFYSLKIK
                     GVKGFVKELTLQPFNHPLFIPFNLLLELVGLIAKPISLALRLFGNLYAGELIFILIAL
                     LTLNATTSSLISTMTLGTAQFILALAWSIFHILVITLQAFIFMVLTIVYLSLAHEDH"
     gene            17241..17516
                     /gene="atpE"
                     /locus_tag="AC06IO_000625"
     CDS             17241..17516
                     /gene="atpE"
                     /locus_tag="AC06IO_000625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIO9873839.1"
                     /translation="MQAAELIAQVQSMTVIAVALLIGLGALGTAIGFGLLGGKFLEGS
                     ARQPEMVPMLQVKMFIVAGLLDAVTMIGVGIALFFTFANPFLSSLGS"
     gene            17568..18038
                     /locus_tag="AC06IO_000626"
     CDS             17568..18038
                     /locus_tag="AC06IO_000626"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIO9873840.1"
                     /translation="MDINLTLIVQMLVFAAFVLFTMKLVWPPLAKALEERQDKIADGL
                     AAAERGRKELELAQHRVKDELKQAKAHSADIIDKANKRASEIIEAAKEAAKREAQIQA
                     KLAQEQIAQQVNHAKEELRKQVAKLAITGAEKILMREVDAKANSELLDNLIEEI"
     gene            18040..18582
                     /locus_tag="AC06IO_000627"
     CDS             18040..18582
                     /locus_tag="AC06IO_000627"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIO9873841.1"
                     /translation="MSDSTTIARPYAKAIFEHALAEKKLSEWSEYLTLLAQVVLTPQA
                     TQFIANPASTDEQQIELLIEICGSKFKKNDALNNLIKLLTTNKRLMLLPEIKALYEVY
                     RAEQEKILEVDVVSYSELTPAQQQRLSESLSQRLSRKVSLKISIDPSLLGGALIRAGD
                     LVIDGSVRGKLNMLGTSLAA"
     gene            18600..20153
                     /gene="atpA"
                     /locus_tag="AC06IO_000628"
     CDS             18600..20153
                     /gene="atpA"
                     /locus_tag="AC06IO_000628"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIO9873842.1"
                     /translation="MSEQVALNPSEISELIRKKIDQFSVVSEARNEGTIVSLKDGIVR
                     LHGLADVMAGEMIEFPGGVYGLALNLERDSVGAVILGDSSTLAEGQKGKCTGRILEVP
                     VGKGLLGRVVDALGNPIDGKGPIESSGMSPIEKVAPGVITRKSVDQPVQTGLKAIDAM
                     IPVGRGQRELIIGDRQTGKTAIAIDAIINQKGTGVKCVYVAIGQKASSVASIVRKLEE
                     HGALEHTIVVVAGASDSAALQYIAPYSGCTMGEYFMERGEDALIVYDDLTKQAWAYRQ
                     ISLLLRRPPGREAYPGDIFYLHSRLLERAARINADEVEKLTNGEVKGKTGSLTALPII
                     ETQAGDVSAFVPTNVISITDGQIFLDVDLFNSGVRPAINSGLSVSRVGGAAQTKIMKK
                     LGGGTRLALAQFRELEAFSQFASDLDDATRKQLERGQRITELMKQKQYSPLTVAEMGV
                     SLFVVEKGYLDDVPVNEISSFEASLHDYMRSTHAALLHAINEAGAYDNEIEAKLKKAV
                     EEFKNTGSW"
     gene            20243..21109
                     /gene="atpG"
                     /locus_tag="AC06IO_000629"
     CDS             20243..21109
                     /gene="atpG"
                     /locus_tag="AC06IO_000629"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIO9873843.1"
                     /translation="MAGAKEIRSKISSINKTRKITRAMEMVAASKMRKTQERMRASKP
                     YANKIYEVIKHIARAASEYRHPFMSEREIKRIGIIVVTTDRGLCGGLNSNLFRETIRT
                     IRNWQEHGKEVDIAVIGRKGQAFFRRVGGNILGSIDHLGDTPSINDFIGVVKIMLDAY
                     YNGTIDSLHIVYNEFINTMTQKPFVKQLLPLPKSEEDKKTLGHHWDYIYEPEAKELLD
                     EILERYIELQVYQAVVENIACEQAAKMIAMKSATDNAGDLIKEFQLAYNKARQAAITQ
                     ELAEIVGGAAAL"
     gene            21122..22498
                     /gene="atpD"
                     /locus_tag="AC06IO_000630"
     CDS             21122..22498
                     /gene="atpD"
                     /locus_tag="AC06IO_000630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIO9873844.1"
                     /translation="MSLGTVVEVIGAVVDVEFPRDSVPKVNDALKLVDSDLVFEVQQQ
                     LGDGVVRTIAMGTTDGLKRGLKAENTGHPIQVPVGKKTLGRIMDVLGRPVDDAGPIDA
                     EETWAIHRKAPSYEEQAGSQELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRV
                     ALTGLTMAEKFRDEGRDVLLFIDNIYRYTLAGVEVSALLGRMPSAVGYQPTLAEEMGM
                     LQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAVD
                     PLDSTSRQLDPLIVGQEHYDTARRVQQTLQRYKELKDIIAILGMDELSEEDKRVVTRA
                     RKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIKGFQGILAGEYDDLPEQAFYMVGSIEE
                     AVAKAKTL"
     gene            22510..22932
                     /locus_tag="AC06IO_000631"
     CDS             22510..22932
                     /locus_tag="AC06IO_000631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIO9873845.1"
                     /translation="MSITTHLDIVSAEHEIFSGVVEMVVATGELGEIGITPGHAPLLT
                     VLRPGEVRITLQGGTQDIYYVQGGMLEVQPHCVTILADVAERAEHLDEAAALAAKAKA
                     EAAIASKGGDIDYSVAAAELARAVAQIRAIQKTRKKMK"
     gene            23557..25812
                     /gene="parC"
                     /locus_tag="AC06IO_000632"
     CDS             23557..25812
                     /gene="parC"
                     /locus_tag="AC06IO_000632"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIO9873846.1"
                     /translation="MKKIPNMNEVTEKKPLTEFTEKAYLDYSMYVILDRALPSIADGL
                     KPVQRRIVYAMSELGLKATAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYR
                     YPFVDGQGNWGSPDDPKSFAAMRYTEARLSLYADVLLGELLQGTVDWVDNFDGTLQEP
                     ALLPARLPNILLNGATGIAVGMASDILPHNLTEVANACIHLLDNPQADIKTICEYIKG
                     PDFPTEAEIISPSETIRAMYESGNGSIKMRAIYTQEKQNIVITALPYQVSGARVMEQI
                     ALQMQQKKLPMVEDLRDESDHEHPTRLVIIPRSNRVDIESLMSHLFATTDLERSYRVN
                     FNMIGLDGKPRVKGLLAILTEWLEFRLATVKRRLQFRLEKVLERIHILEGLLIAYLNI
                     DEVIAIIREHEQPKQGLISRFNLTERQAEAILEMKLRHLAKLEEIKLRGELDELSDER
                     DTLQKTLASHFLLKELVKEEITKDRDEFGDPRRSPIITREDSQALKEEDILPNEPITV
                     VLSKNGWVRAAKGHDVDGKELSYKAGDEFKAQTLARTNQQVLFFDGEGKVYSLPGHVL
                     PSARGQGEPLTGKLNPAEGVLFEAIVGGEPEQKVLLATDSGYGFIAKIEDLYVKNRNG
                     KACIKLSENSHILPPRLIPNREQLFVACATNVGRLLIFSLEELPELSRGKGNKLISIP
                     AAKAVSREEYIVDMQVLTTNDSLTVYAGKRHFTLKGEDLTHYKGERGRRGNRLPRGLQ
                     NVSQLHVTQSE"
     gene            complement(25919..26773)
                     /locus_tag="AC06IO_000633"
     CDS             complement(25919..26773)
                     /locus_tag="AC06IO_000633"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIO9873847.1"
                     /translation="MNFNIIGAGRLGKNLGIAMSVHQIASLNSICNSNLASARIACNE
                     IGFGNAVGKISDLSEADITWITCRDDSILDVVSILDNIEILKKGSLVIHCSGALNSSV
                     LLPLKKQGCYIASVHPLKAFRTNSLDSNAFKQVHCVLEGDYEACRWLRFAFEKLEACI
                     IAIQPEMKVIYHTAATIASNYLVTLASYSEELLLKAGIQQVQSRKMICDLMYSNILNL
                     NQVDSAKSALTGPLTRGDMQTISLHLEAIDNSEIKRLYKNMALATLPLLDLSLDRKES
                     LRKTLEKE"
     gene            27026..28687
                     /locus_tag="AC06IO_000634"
     CDS             27026..28687
                     /locus_tag="AC06IO_000634"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIO9873848.1"
                     /translation="MKKRIVSLFVISTVISSVNAATQAIVWGESTKALPQFMQAHPFK
                     KQGLLQTVKSQQSDYKLELQNNSNHATRHARYQITYKGVPVWGYQVIFHSKDGAKETV
                     TGMNITGIEQDINSIEGKLSPNDIEQKILGTVTEPVKFKNLKKVIYIDKGNKAHLAYH
                     LSYYSNSEKKHVNAPNYLIDANSGEILKQWDEVRHERISQGLGGNAFTLPYRQGMFQH
                     GNALPGLPSLGKFDVNVEDGLCRVENESIKVMNLENHNIGYDFFPVTIFAESVLNLSA
                     FSYPCNETNLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQKVYGVNRPIGDDLP
                     IRAYTHLGDMDNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTA
                     LHSGLMYEGQSGGINESFSDMAAIALLDYLSKDYPWYWDGEDWTIGREAVKSGQPIRY
                     LDDPAKDGVSIGHASEYTDALDVHITSGVFNKAFYLLAHKPGWSIRKAFQVMVDANMN
                     YWSPIAYYDFAACGVIQATIDKHWDKTPVIEAFAEVGVVCPMHKS"
     gene            29142..30671
                     /locus_tag="AC06IO_000635"
     CDS             29142..30671
                     /locus_tag="AC06IO_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873849.1"
                     /translation="MSITAQELVKQYKLRLTPAMENDLLSEESRLKKELEAVPFNSEE
                     TLYKSILQMIIVFYEENTLEENRDLLQDHELIKQLSALMWDDIQIKLIPFLIQKNFTL
                     SEIKELLFDEAYYRSLHVLVDFSLTQDIPELLAHQEKREQLKFINTLANDHCRKLCLI
                     FWVKGSLSIKEIQDIVNATSHYPMLAETLIALDKTKTISIKQLKKLALDPKKHQQESI
                     LYHYSEQFKAYNLRKSDLSQLNLDDLDALGKSFKVLKEAGIANDYAYRLVLKNNKTGQ
                     LLRLFLPELAKIESLSHRKALIELLYIGAQKGVVTQGKALLQIKDSNLLALARALRER
                     FICVQQMQDLGFKKEIIAFTGEENNINSSRFRHVIMRVEEKCKDIHERLRKSSLDKDK
                     VGNWQRADEKYRQTLYSIAYDGITKSGVDLHIKMKSAEKEILSIVDPEIKSIIHKVLV
                     VIANIIITALTLGFANDLKESATGNYWFFNQSPSGEVIRALNKEVLTTIDSPELITIS
                     P"
     gene            30934..33549
                     /locus_tag="AC06IO_000636"
     CDS             30934..33549
                     /locus_tag="AC06IO_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2975 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873850.1"
                     /translation="MGLPKKALKESQLQFLTAGTAVSDSSHQTYKVSFIENGVIKNAF
                     YKKLDPKNHYPELLAKISVAVSLFKRIFQGRRSAEERLVFDDEERLVGTLSISVDGFK
                     GFNFHKESVPQESSAKEQVIPSTRTLIEKSFMEILLGRWFLDDDDGHPHNLSLAGDID
                     FDMFFYWFTIYMKEPRPAIGIPKTRVNLTVRDWEGFPNVKDSKPFHWPTYKNPGQETL
                     PTVLPVQDKLVNLILEKTYPDPGQFEQLAHEPVAQEQKFAAALKILLTYQPEMIRKRL
                     TELFGEMTLNYTSLDETDVALRSQYEKTFPHLCNENTNIKPFVDFIMNLYQMHYDNLY
                     RVVVFYMGCENNGYGVPLPATNSALYHKPSFYKDIVEWARTQNITIFSKDDSSIKFDE
                     DELRRRYHQVWRDAYAPTFRDLLHDSYSLTNKLLQQVSTFHVVLDEVEGKKPTDDTLT
                     NAWELFGTMPELSLEKITPLISVDKDSKLRTALILLVEFTTQFHAVAKAYYQKDRKDL
                     TEEDNLEFSEQLVQLYTNYNLKIRQSLAHTSTLAGEFNRIAVGLKQYTERANFQLHLT
                     TTDEQMKEATVATTPKEILPHTHEDVIRQFNDSLFLWAKNSRPEDLSHHISEIIDKYY
                     APTIELLSKRHRAQPVKEYLQASVNESGENRLAYILSAGEGDTGALNTLLIQHLTPYM
                     LQTYPLLSIRNAVKEGGFDKDLEIFTKAAVDFAKHDRRFIHLYNVEGKSLFFKTMYEW
                     IDELPATKFKGLLESALKDYEGKLWWSTSRRSEVEGYCTKFSQAKIVAMTFLNGKDSS
                     SLNDVLFDKIIAAIQKDINKNKEKLKVPGFRLINCYNAKEHRADYFKEVKNYAEPVSH
                     RQETTLNSNITSLVV"
     gene            33642..34493
                     /gene="asd"
                     /locus_tag="AC06IO_000637"
     CDS             33642..34493
                     /gene="asd"
                     /locus_tag="AC06IO_000637"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842959.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HIO9873851.1"
                     /translation="MFRDVLKTLPQYLIPKHGITALAGYFADVKNPRLKNFLIRNFIR
                     KFDVDMSEALIEDPKSYDCFNDFFIRHLKPECRPLSQSDVICPVDGCISEIGKIERGQ
                     LLQAKGKYYSVQELLACDGQLAEQFVQGQFATLYLSPKDYHRVHMPIDAELVSMTYIP
                     GALFSVQPATTRVVPKLFARNERLAIFFKTKIGPMVMVMVGATIVGAIGTSWHGDVKR
                     SKKLERFDYSEQFSDKIISQGSEMGYFKLGSTVVLLFANGEKIQWDKELLAGSKIQLG
                     KPMAIIT"
     gene            complement(34498..34584)
                     /locus_tag="AC06IO_000638"
     tRNA            complement(34498..34584)
                     /locus_tag="AC06IO_000638"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34548..34550),aa:Leu,seq:cag)
     gene            complement(34667..35635)
                     /locus_tag="AC06IO_000639"
     CDS             complement(34667..35635)
                     /locus_tag="AC06IO_000639"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIO9873852.1"
                     /translation="MSIITTNLNRAIHDLQQGKPVAIPTETVYGLAAPINNNAAIRAV
                     FEMKSRPLNHPLIVHIASNWDLHDLVEEVPEYAHQLADKFWPGPLTLVFHCKKDQINP
                     LITGGQSTIAIRCPDHPLTQKLLQKLNIPVVAPSANPFGKISPTTALHVKQSFNDAEL
                     TILDGGRCRVGIESTIVDATNPDSYQILRHGIIDEKTIAETIAIGCSTQKNDIRVPGK
                     LDSHYQPQKTLYYFDQYETLSEFCQKRKGTVYVIANRQPPGVETNLFHPLGMHPEKSA
                     YDLYYQLRLADTSDAICIAIELPPNTSEWQGVRERILKAGFPYSQQ"
     gene            complement(35865..37535)
                     /locus_tag="AC06IO_000640"
     CDS             complement(35865..37535)
                     /locus_tag="AC06IO_000640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIO9873853.1"
                     /translation="MAIKVTKRGMDLLRDPILNKGTAFSLQERDEFALHGLIPTTVET
                     LEQQVIRCLDAYSAKENPLEKHIYLRALQDRNEVLFYRFIIDNLVDILPIIYTPVVGQ
                     ACEMFSHIYRQPRGVFLSYPERDKLDSIIQNIASTRSIKVIVVTDGERILGLGDQGAG
                     GLGIPIGKLSLYTSCGGIHPSNTLPIILDVGTNNKERLDDPEYIGWRHARISGKEYDD
                     FVDQFVQSIKRHMPHVLLQFEDFAQQHAYPLLERYKNQLCTFNDDIQGTASVAVAAIL
                     AATRVTNTPLKEHRVALLGAGSAGCGISEQLVHAMMNQGLSEEEARSRFYLVDRYGLL
                     HDEMTDLLPFQKGFVRSSTSLQNWKLEKKGEITLTDVINNAQPTILLGVSGQPNQFKE
                     AMIKTMLSYCERPIIFPLSNPTSRAEAIPQDLLNWTAGKALIATGSPFEPVVINGHKI
                     EIAQCNNSYIFPGVGLGVVAGQAKRVTDLMMMAAAVALSELAPAIRTGEGRLLPELNS
                     IREVSQHIARAVILQGIKEGHIEPMNNNKIDESIKRTMWTPQYEPYVL"
     gene            37787..38569
                     /locus_tag="AC06IO_000641"
     CDS             37787..38569
                     /locus_tag="AC06IO_000641"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIO9873854.1"
                     /translation="MLLKCIEKIVDGYFAVIPRHTPEFENINGALIIAHRGAHNNARG
                     IIENTLEAFQNAKETGCWGIELDVHLTKDKVFVVNHDPTLNRLWNKDIAIADLNFSEL
                     RALECRIPSLSEIVSQYGNSMHLFIELKTAIEEEEILVQTLQHLTPCKDFHLLTLQES
                     TFKKLCSFPRQSLLLVAVHNNVKKFCELSIKEGYGGVLGNYLLLTNRALKYLQDANQH
                     FGVGFVDSKNSLYRELNRGVKWLFTNEAININQYLNDLKNSM"
     gene            38721..39488
                     /locus_tag="AC06IO_000642"
     CDS             38721..39488
                     /locus_tag="AC06IO_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIO9873855.1"
                     /translation="MTLKITFLGSGSSFAIGPENYQSNVLLELENDTLLIDAGTDIKY
                     SLRDQGLTYKDIKNVYISHLHYDHCGGLEWFALITYFDPNHSHKPCLIASDKIINELW
                     DKSLSGGLSTLPHERANLSAFFDVKPVKQYEGFVWQSIKFKLVQTVHYYSEYELMPSF
                     GLIFNYNKSRILFTSDTQSCPVQLISFYEESDIIFHDCETTESKSGVHAHYSELVQLP
                     PHLKKKMWLYHYNPGKLPNAKKDGFLGFVAKGQSFVF"
     gene            complement(39541..40710)
                     /locus_tag="AC06IO_000643"
     CDS             complement(39541..40710)
                     /locus_tag="AC06IO_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIO9873856.1"
                     /translation="MSLITSYSPMPVTFTHGEGVWLYDERGKKYLDGLSGIAVCGLGH
                     AHPDVTRTIQEQAARLIHTSNVFHIKEQELLAAKLTSMARMEQVFFANSGAEANEAAI
                     KLTRLFGHKKGIETPSIIVMEKAFHGRTMATLTASGSRKVQAGFEPLVPGFIRAPFND
                     LDAIHTIAANREDVVAVMVEPIQGEGGIYPAEEGYLRALANLCEQNDWMLILDEIQTG
                     NGRTGKLYSCMHYDIQPDILTTAKGLGNGMPIGACLMSKRAKDLFKPGNHGSTFGGNP
                     LACATALTVLEVIERDKLCERVTKNSALLMDKLISNLGEHPHVKAIRGKGYMIGIELD
                     RPANDMRVLGLENQVLFNITAETVVRLLPPLIISEDEIDELVDRITRTINQFTNQ"
     gene            complement(40769..41347)
                     /gene="sodB"
                     /locus_tag="AC06IO_000644"
     CDS             complement(40769..41347)
                     /gene="sodB"
                     /locus_tag="AC06IO_000644"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HIO9873857.1"
                     /translation="MTFTLPQLPYALDALAPHVSKETLEYHYGKHHNTYVTNLNKLIP
                     GTEFESMTLEEIIMKAKGGIFNNAAQVWNHTFYWHSMSPNGGGEPKGRLAEVINKSFG
                     SFAAFKEQFSQTAATTFGSGWAWLVQDQSGALKIISTSNAGTPMTEGLNALLTCDVWE
                     HAYYIDYRNRRPDYIEAFWSLVNWDFASSNLK"
     gene            41678..41995
                     /gene="grxD"
                     /locus_tag="AC06IO_000645"
     CDS             41678..41995
                     /gene="grxD"
                     /locus_tag="AC06IO_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIO9873858.1"
                     /translation="MDTLEKIKKQIAENAIMLYMKGTPKMPQCGFSARAVQCIEACGV
                     DFAYVDILANPDIRQVLPQFSDWPTFPQLYVKGELIGGSDIIAEMFQQGELEPMLRDA
                     VAA"
     gene            42048..42653
                     /locus_tag="AC06IO_000646"
     CDS             42048..42653
                     /locus_tag="AC06IO_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIO9873859.1"
                     /translation="MSVLVGRKAPDFTVAAVMGNGEIVDKFNLHEHLKGKYGLVFFYP
                     LDFTFVCPSELIALDHRIEEFKRRNVEVVAVSIDSHFTHNAYRNTPVKNGGIGPVRFT
                     LAADMTHSICQSYGVEHPVAGVAFRGAFVIDTNGMVRSQIVNDLPIGRNIDEILRIID
                     AVQFFEENGEVCPAGWQKGQAGMKASPKGVAEYLSEHSESL"
     gene            complement(42779..43402)
                     /gene="rnt"
                     /locus_tag="AC06IO_000647"
     CDS             complement(42779..43402)
                     /gene="rnt"
                     /locus_tag="AC06IO_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HIO9873860.1"
                     /translation="MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIV
                     LLDMDEQGLLKKESSYFEHILPFPGAELDQKSLEFNQIDPYQPLRFAVDEQTALERLF
                     KPIHDLLKKTRCQRAVLVGHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIF
                     GETVLAKAVQAAGLHFDSQEAHSAIYDAEKTADLFCLMINKWRMLNI"
     gene            complement(43386..44423)
                     /gene="pyrC"
                     /locus_tag="AC06IO_000648"
     CDS             complement(43386..44423)
                     /gene="pyrC"
                     /locus_tag="AC06IO_000648"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIO9873861.1"
                     /translation="MQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKP
                     ALTNLPAIESYRNRIISAIPKQNSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYP
                     AGATTNSEEGAKSLKALYPLFETLQTSNLVLQIHGEVTHSDIFEREALFIDEYLKPIA
                     KHFPKLRIVLEHISTKAAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLP
                     ILKHEKDQKALQVAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPYAVALYTQI
                     FDELNQLKKLNDFLSRFGAEFYQLPINQQQLELIKSPQTIPNSMPFGSTHVVPIAAGD
                     TINWSINGFSQ"
     gene            44493..45377
                     /locus_tag="AC06IO_000649"
     CDS             44493..45377
                     /locus_tag="AC06IO_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0097588 - archaeal or bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein MotY"
                     /protein_id="HIO9873862.1"
                     /translation="MISDHFICKKAKVIAMSSLFITTVVFAAPSIQYASPMGEENWRM
                     TGNPLRCGLSLTIPNYGIGYFEQYATQSPHFILRKWDQVQRALPAQVVASPPVWKPLL
                     KGSYLVTRTYIKPGEYGLFLNRQPALKLLTFLSQGYQANFSYLSEEGFRTTVILSPVR
                     FQKVYSQYQRCLGGLLPFNYEDVKESVFHFGVDSKLLSDADKVQLRRIAQYVDADVQI
                     DVIKIIGHADDSGRKGYNNAISQYRAESVKNYLIRLGVPQRKLSITWVGALQPIARND
                     TDEGRAANRRVVVTLIKK"
BASE COUNT        14215 a         7986 c         9427 g        14126 t
ORIGIN      
        1 attacacaaa aaataatcag ttattcaaat tgacagttga aaaaaagtta tctatcattg
       61 ccaacctcat aaatttagaa gcagggttat catgaaacgc acttttcaac ctagtaattt
      121 aaaacgtaaa cgcgatcatg gttttcgttt aagaatgtct actcgagctg gtagattagt
      181 tataaaaaga cgtcgtgcta aaggtcgtaa gcgtttatct gcctaagtgt ttgcatttaa
      241 aaaagcgcaa cggctattaa aaaaaaatga ttttgatttc gtgtttgaaa gcgcgaaaaa
      301 aataactaca gatgacttta ttttcctttt cagagaaaat aagttaggct atgcacgcct
      361 tggtttagca ttgtcaaaaa aaatgatagc taaggctcat gacagaaatc gtattaaacg
      421 gctacttaga gaaagttttc gtcatactaa cttgcctgct gtagatataa tcattttggc
      481 aagaccaggt ctagcaaaga aaaccaattt aggtataaat actaaattaa ataaaacatg
      541 ggaaaaatta acctcatgct acggcaaata gtttgcttac caataaaaat gtatcagtat
      601 tttattagcc ctttgataac accatgttgt cgctattatc cgagttgttc agagtatgct
      661 gacagtgcga tcaaacatta tggcgtaatt aaaggtttat tgatggcact taacagattg
      721 tctcgttgcc atccatggtc aaaaggtggc tatgatcctt tatttcctaa cgataagaat
      781 tgagaatatt taatggatat acgacgtata gttttatata tggcgcttgc gcttattggt
      841 ttatcacttt ggaacgcttg gcaaattgat tatccagcca aacaaccagt tgaagagaaa
      901 acagcaagcc aattaacatc agatggacat ttgttaccac aaataatacc ttctaatgcg
      961 gagcaacccg taactttgaa agcagaggaa aaagcaagca gcggtaaaca attgattcag
     1021 gttaaaacag atgttttgga tgtcgacatt gatttaaaaa atggagatat tgttaaaggt
     1081 ttattgcttg actatccact aagtgttgaa gataaaaata aaccattccc tctcttgcaa
     1141 aatcaggcca gccagagata tgtagcgaac agtagccttt ttgttttaga tggccagaca
     1201 ccacaatcat tggattttga ttttacatca gaaaaagaat attacgaatt gaaaccggat
     1261 caaaaccagc tgatagtcac tctgaatggc aaaagcgaag acggtttgga tgtgaaaaaa
     1321 gaatttgttt ttacaaaggg aagttattta atcgaagtca attacaagat agccaatata
     1381 ggtaattcgt tatggaaagg gtattttaat acccagttat tgcgcagttc tcccaaagaa
     1441 gacaaatcga gcattttcca tattggctcg tatacaggtg cttccttttc aaatcctgga
     1501 aaaaatcgtt accaaaaagt tagcttcagt gacatgagca aatctaacct ggatgtggat
     1561 gcaaaaggcg gttggattgc tatgcaacaa cactattttt tgagtgcttg ggttcccaat
     1621 gcggatagtg aaaacaagtt ttatacatta gctacggata aagattatac tattggagca
     1681 gtaagccagc ctattacggt caaaccaaaa gaagataaaa ttgtaggatc caaattatac
     1741 atagggccag aaattaccag tgttttaaaa ggaatatccc catctctgga tttaacggtg
     1801 gactatggca ttttgtggtt cttgtcttcg ttattatttt ctttaatgaa agcaatttat
     1861 actgttgttg ggaattgggg ctggtcaatt gttcttgtga ctgtcctaat taaattggca
     1921 ttttatcgat tatcagctac aagctataaa tccatggcta gtatgcggaa gttgcagcct
     1981 aagttgcaag ctttgcgtga gcgatatggt gatgataaag ctaaaataag ccaagctacc
     2041 atggaacttt ataagcaaga aaaagtaaat cctttaggcg gttgtttacc tatattaatt
     2101 caaattccag tttttatagc gctctattgg gttttactgg aaagtgttga attaagacaa
     2161 gctccattta tattttggat aaatgatttg gcttcagcgg atccttacca tgttcttcca
     2221 ttaatcatgg gagcaaccat gttgattcag caaaagctca atccagcacc agctgatcca
     2281 atgcaagcta aggtaatgat gtttttaccc atattattta ccggactgtt ctggaatttt
     2341 ccttctggtc tggttttgta ctggattgtg aataatactt tatcaatatt acagcaatgg
     2401 tacatcacca gaaaatactc agatgaaaag ccagctaaaa aggttgttgc cacagcaaaa
     2461 taaatgtcaa tagataccat tgttgctatc gctaccccgc caggacgagg cggggtagga
     2521 atcgtcagaa tatcaggtcc aaatgcttat gctatagccc tttgtctaaa tgggaacaaa
     2581 gcattgcaac cacgtttagc cactttttgc tctttatata aaggaaataa tgaagtcctt
     2641 gatcaaggat tggtccttta ttttaaagga cctcattctt ttacaggcga ggatgttatt
     2701 gaaattcaag cgcatggctc acctgtagtt ttggatttat taattaagga atccatagca
     2761 gcaggggcaa ggctagccag gccgggtgag ttttctgaaa gagcatttct taatgataaa
     2821 atcgatttaa ttcaagcaga agccatagct gatttaattc aagccagttc agataccgcg
     2881 gctcgtatgg ctttaaaatc actgcaaggt gatttttcta aaaaaataaa tcaattgaat
     2941 gaagagctaa tttacttacg catgtatgtt gaagctgcta tcgattttcc tgaagaggaa
     3001 attgattttc ttaatgatgg aaatgtcagc caattactac aaagaattat tggacgatta
     3061 gaagagatac gttcacaagc gaatcaagga gttttgttgc gtgaagggtt atccttggtt
     3121 attgctggca ggcctaatgc aggaaaatca accttaatta acaacctggc agggagagat
     3181 gttgccattg ttactgaaat tgccggtact acgcgagata ttatgcgaga acatatccta
     3241 ttagatgata tccctctaca cattatcgat acggcaggat taagagacag cgatgatcta
     3301 gtggagaaag agggaataaa acgtgcttgg caagagctaa agagagcgga ttgtgtgctt
     3361 cttgtagtag atattaacaa tccggatcaa caaaattctc tgcttaatga attgagatta
     3421 acattaccca ataaaatacc cataatcacg gtatacaata aaattgatac tactaaactt
     3481 acagcaaaat gtgatgagca tacggtttat ttgtctgcaa aaactggtga gggactggat
     3541 gagctaaaaa aagtgattaa acaagtagtt ggatatcaac caacagaagg ccaattttta
     3601 gctcgcaggc gacatctaca agcgctggat gaagcaaaag ctttgttact aactgggcaa
     3661 tcgcagctaa caaatcataa agctggagaa ttgttggcag aagatttacg gttagctcat
     3721 cagactttat gtgaaattac aggtgagttc acttcagacg atttattagg aaaaattttt
     3781 tccagttttt gtattggaaa ataacgatga tgcaaggctg tcatcatgtg aattttcaat
     3841 ttttcttctt ttgaatacag taaaattatt attaaatatt ctgtggtgat gtgctttatt
     3901 gggtcaataa atttattctt ttagtttcag cacatgtcaa atgaaccagc ttttaccttg
     3961 atttattttt atgcaaaacc aatctgaatc gcaaaaatca atgtgtaaaa tattaataca
     4021 aaaaagcttg tcaaaataca tatcaaatgg taacgtatta ttgtaaaact tcctgtgtaa
     4081 tttttgtgag ttcttaatat ggtctactca aaacaaagtg ctgcatttcg tagggataaa
     4141 caacaatttt tcgcccaaat tccgaaggat agtaaactgg acacaaaaat tgtaacaatt
     4201 gatgatcgag taaacactat agattacact tttttactac accacaaaca ggatttagaa
     4261 gctgaattca ctcaaatgtt tactgtgtta cagcagcaag acgatgaaaa taaagaaatt
     4321 ttctggctat attgctacta ttgtgcatct ttgctggagg ctttccacaa agcttattca
     4381 caaaacagta aacaagctga atacgctaaa ataaaacaaa aaataaaaga ccatttaaat
     4441 aaggtcaatg tccaagagga agaatctggt tttattcagt ccttgtacga ctcctttctt
     4501 ggaggttttc gcaatttaat cagctctcca ttgcacgctt ccaaaatcag agattatgtt
     4561 gcctactcga acttgtgtcg tatctattgg acgttttgtc gtttgacttt aactaacgga
     4621 ttgagcgtag caagagacct aaaaattatt gagaagttgg atgctatttt aggaactcat
     4681 actgatgtcg ataagattat ttcaactctt caagcaccta ccggggttat aacttacttt
     4741 agtgttggtt tctttttgat gcgcttaata attgatggtg gtatgctatt aagacatacc
     4801 tttttcccta ctgaattgga aaaaggcgca gaaaatggtt gtgaaatcca tcaaatggat
     4861 tatttgccag gcccagccag tctcgaagca tatagaaaaa gttatattct tgttcatgaa
     4921 gacaatcaag aagtatcact ctattacatt cctagaagtg gtaaagcaga aaaattaagc
     4981 attaaagaaa ataacaaact gaaagatgct ttactgaata aaatgaataa ggacacatca
     5041 tcccttcgtt tgagctcaac tgaagtagaa gagataatca ctcatcaaac agggcatgtt
     5101 cccgaagtga ctacggcatg ggacagattt aaacatgagt tttataaacg acactgtaat
     5161 tttgccaatg actttgtttg ggctacaatc aacctgctta ccaactataa ttaccttttt
     5221 aatatttccg gtcctgcagc agggatgatc acttctgttt ttctcgggtt cgacgtatgc
     5281 atggctttat ataagtgcaa cttagccaaa caagagtatt tgattaagaa agctcaatat
     5341 ttgcaggaaa tagaagacta taataatccg gaaaaattca aaaaaatgac tcctgaacag
     5401 cgattattgc atatagagat gctcaataag caacttagag agcttgaaat taattataaa
     5461 acccaggagg ctacttttta ctttgttgcc gctgctgccg ccttattgat gatgggattt
     5521 actgcatcga tgctggttag cccgcctgta ttagtcgttg gttgtttttt tgcctgtact
     5581 cttgctgttg caatgtatct gtctacggga tcttattcga aatacgagga aaaaagtttg
     5641 tatttggaag aggctcaatt gacaggtaaa aaccttccag ttgctcttaa agaatacgaa
     5701 gcagctcgga atgattttat ttttaccatg gtaaaaaata cggtcatgcc tatagtttta
     5761 attacaacat ttgcaatttg ctggcctgct gcaattgttt taacagctat gtacattggt
     5821 tatgaaattt tccatgctta tgaccaacac tctgaagcga aagcagccaa acagttggct
     5881 ttagccgctc cttcagagga aaaggatgga tatatggctt tagccactcc caattaggtt
     5941 tcagggtaca ttgctttgac ggcagcgata tctttttcac ttaaatgagt tcgctgccct
     6001 atttcaactc catcaactag aggtatgata gttggttggc cattcttgga aaaagcaaat
     6061 cgaccataat gcataatgga tccatagtca tattcaccga aatctttccc atcagttaaa
     6121 tgttggtcaa aattaaattt atgctcctca tcaatatttt cccagacaat acgtatatat
     6181 tgatttctgt ctgatcggga ttgctcatgc cacatcccca aggcatgccc tatttcatgt
     6241 accgtattca tggtcgtaca tcttggcgcc aaattgatct cttgtttccc tccctggcgc
     6301 ccaacatagg aagagcatgt tgtgccttct gccgggataa aagaaatgta atcacgatag
     6361 tcatagcgat ttttagaagt taattcaaca aattctaaat tcgtattttt ctgccaatga
     6421 tctattgctt gtaaaatggc aagcttgttt ataaaaggta aattttcagc aatttcataa
     6481 gggataacac catttggcca gcgtgaacct cctacttttg gtgtaataac agcaccttgg
     6541 tttttaagat tctctgcttt accaatcaaa atatcacctt ccactactgc aaattctcca
     6601 acttgttcat aggtaatggt gcgactgccc ataaccggat cagctactgt gatttgatgg
     6661 agattaattg cataagaaga ggagaaacat aatgaggaat aaaacaaact tgcaatccat
     6721 gatttagttt tcatatatag tccttaagtc taaaaaaacc aaggacagtt taggggaaag
     6781 agggagtaga tttccagaac tttttatata gtttgttgac tcttataacc ttaatgcaag
     6841 gacgccttta caaaacgtcc ttaattgata attatgcgcg attacatgaa taaatagcta
     6901 taagaatata gcgatgcgaa aatatataag gttaagttta aatatttttg caaagagtcg
     6961 gtgtaatgtt gttaaccgga gcactatctc cattttgatt aaagaatttt aaatgcttat
     7021 ctggttattc gaataattat taatatcctt agttttatga acaatagctt gtgaagcaat
     7081 atccacgtca agaaaaaatt atgcagaatc tttaatgaat tggggctgct attttaattc
     7141 gatcagaaat gtgaaaaact ggatttttct gttgtttagt aaaataatcc tttggttttg
     7201 aaatgaataa gtgagaggta ggatgactcg aagtcaattg attttggcta ttttggcagg
     7261 attggtcata agtattgaca atatgattag tattgttgcg ttttcctcac ttatttacca
     7321 aggtgcgtta gaaatttata ttccgttggt cattaatata ttggcaattt cagtaattat
     7381 tataagcgcc agctatttgt tcatgtcttc ccctccttat gcaatcgctc agttgcaaga
     7441 tgaagcggca attttgtatt cagccatggg tttagtgatc attaaatcga tgccatcaag
     7501 ttctacgaat gaagccatct ttattacgat tctctttatg cttggagtca ctacctgttt
     7561 aacgggctta tcattctact tcgttggaaa atttaggttt ggaaatatta tccgttatct
     7621 gccatatcct gtgatttgtg gttttatggc agcgacaggc tggctagttt tatcaggaac
     7681 atttgcatta ttaactggta aagcgctgca tcagaattgg cttgtttttt ttcataaaga
     7741 ggaaatatta ttgtgggcgc caggttttat tgcgggaata gttgtttata tttttaaaga
     7801 aaaattaggt tatcgatacg cgcttcaatt gatgtttatt tctcttgtaa ttctattcta
     7861 tgctcttttt tatattttta atttggagta tttggtcgat ccaaccgaag ggtacgtctt
     7921 gggacctttc aatcaaagcc acattgattc gctcatgaaa cctgaaatat ggcaaatgat
     7981 tcattatcca ttctcaatga ccctattgaa ttacataggg ttagttatac tggtaagttt
     8041 tattgccttg ttattaaacg caagcagtta tgaattaata gttaataagc aattggattt
     8101 aaacaaggaa cttcgtatag caggtattgg caatgtattc agtggattac ttggaggaat
     8161 ggttggctat ttgtctttgt cagcgtccac tgttgcaaaa gagtatggcg gtgctcgtat
     8221 tataggggtg atggctttcg ttcccatgtt gcttattttg tgctttggtg ctgtggtcgt
     8281 tgaatgtttc ccgaaaatgg ctatcggctg ttatttgttt tatatcgctt tttgctttct
     8341 atctgaatgg ttgatcaata cctggcgttt attaaattta agtgaatatt taatcgtttt
     8401 actgatattg gttattgcta ttgtctttaa tatgattacg gcggttgcta taggaacggt
     8461 tatttcaatt tttatcttcg cttttcgcta cagcaaaata aatccggtta agtatttgtt
     8521 ttccggagca gattataatt cttcttttga gagaaatcct atatctcaat ctattttatc
     8581 gacacaggga gatgaaattc agtttttgaa gttgcaagga tttctttttt ttggctctat
     8641 ttacaggcta cttcagttaa taaaaaatat acagaaggct cgatatatca ttttagactt
     8701 tgagcttgtc tacaatatcg actcatcccg cattatttta atgaaaaact taaagctcca
     8761 tgcggaaaag aataatattt cctttgcaat ttgttcatta aagcccattg tttatcatga
     8821 attaatgaaa accaatttat tacaatcgaa aaccaattgg cttaaagtct ttactgatca
     8881 agaatcagct ttagagtggt gtgaagatca agttatagaa aaatatacag aaaatataaa
     8941 tattgaagaa gtaacacttg agaaacagtt actctattta ggtttttcct ctgaactggt
     9001 agcatggatt agtgaaaagg cttctataaa accttataac tctgatgata tcatttgtca
     9061 tgaaggagaa gagtcaaaca gtgtttactt tattcatcgg ggaagagtta cagcatttgt
     9121 cggtgataaa cgtgtacttg ttgtgggttc aggtaatgta cttggtgaaa ttgccatgta
     9181 cactcataag aagcgcacag caacattaaa aaccaatgaa aaaagcattg tttatgaaat
     9241 tgatttgaaa tcaatccagg agctttctaa aaataaaccc ctactcttgg ttcaatttca
     9301 cgcatgtatg gctaaaattt tggcaaatcg tctttcggta ctaaataaac ggataaaagt
     9361 ttttgattcc accgccatat tgcaataaaa gttaataaaa tattctttta ctgtgaaata
     9421 ataataagga acaacgtttt aaagttgttc caattgtctt aactgaattc gataggcaac
     9481 aactctctta tccatgatgt aatgccagag cggtggaatt aaagctatga taatcattcc
     9541 aaggtaccct gagggaagtt gagggctgtt ctccatatga cgtaaaattt gatagggtcg
     9601 tgcgccatgg gcatggtgat ctgaatggcg ttgtaaatga attagcagct gattacttag
     9661 ccaatgatta gcattccatg aatgatttgg atttaccttt tcatattggc cgttactcag
     9721 cattttcctt tccagtccat agtgctcaat ataattaaca atttctaatg tcaggatggc
     9781 aataaatgat tgtaataaga aaaatgacag agcgattaat cccccaaaaa tgaaagtgac
     9841 tatacaaatc atgaatggaa cagtaaggat ccaccaaaaa ttattatgta tgctccagat
     9901 ggaataattt ttatgcgata aacgcttttc ttccaaaagt aaggcagatt gaaacgaacc
     9961 taataatgtt tttggtaaaa actgatataa attttcgcct aaccgtgctg tggcaggatc
    10021 gtcaggcgtt gctactttaa catgatggcc tttgacatgc tcgataaaaa aatgaccata
    10081 gcaaactgta accaataaaa ttttactcat tagttgttgc aatttactgt ttttatgcat
    10141 catttcatgt gcaaaattga tgcctactcc acctgagata agtcctagtg acatggtaaa
    10201 tcctaaccat tcattccaaa tcaaattttg agtactaact atatagactc cataagcaat
    10261 gatggttatt tgtataggca cataggcgca agtaatatat ttaaagaact tatcttttag
    10321 gagttcttgt tcctgctctt ttgttggatt taccgaatca tgaagaaagg catcaattaa
    10381 tggaataaaa gcaaaaacaa taaaaaaggg tagaaaactg taccagcctc ctaaatataa
    10441 agagacaaag ggtaatggga taagtaaata gaccagtaaa aaactatatt tttttaaatc
    10501 atccatttta taattccaag catagcctaa tatagaaaca atatagctta aaaggcgata
    10561 tctttgcttg gccttagagg acaaaaaaag gtcatttacg gacaatgtta tggaaataag
    10621 agatcaatat tctttttagc tcccttttaa tttcaatgcc tcttcataga gctgattttt
    10681 attggcatta gtcaatctgc atgcaatagc cacagcctgc tttaaaggca actcttccaa
    10741 caatatcttg agtagttctt catgcgaatt gaggtcttta tttgttgagc gaggtggaaa
    10801 aatcaaaaca aattcacctt tgatatgacc gggttcactg agtagccagt ctttaatttc
    10861 ttttattttt ccagatacaa atcgttcaaa ggttttggtt atttcttttg ctaaaacaag
    10921 ttcataatct tggccataga tctctgcaat atcgttaaga cagtctatga ttctgtgagt
    10981 tgattcatag aatactgtgg tataaggcac tgaccgcgat gattccaggg catcttttct
    11041 ggcactttgt ttggccggaa gaaaacctaa aaaggcaaaa gagtcgcagg gtaccccggc
    11101 agcacttaac gcagcaatta aagcgcatgc gccaggaaca ggtacaacag ggatatggtg
    11161 ctggcgagcc tgtttcacca aaggaaatcc aggatcgcta atcaaaggcg tgcctgcatc
    11221 actgattagt gcgattgatt ttccatggag cagcagctca attatctcat tgcttttgtt
    11281 cgcttcattg tgggcgtgca atgaagttaa attatttttt atccctagcg aagtcaataa
    11341 ttgcatggaa tggcgtgtgt cttcagcaag tattaaatca acgcttttca aggtattaag
    11401 agccctgaaa gaaatatctt cgcgatttcc tattggggta gcaactatat agagagttcc
    11461 taaacctgtt gctagagtgt ttgtcatagt ttatcctctt gcgaaaagca aatttggcga
    11521 attaacatgt taattaaaca attaaaactt cgcatgttct tagtattggt atctcttttt
    11581 ttgctttgcc aatgcacaaa aacagttaac tcaaccgagc ctgtagtaac agttaataaa
    11641 aaattaaaaa ccccatattc attacctact gcagcatact tggctatggc taagagtcag
    11701 caaggccatg aaaagcaaag cgcattgatt tccgcagctg gccgtttaat tactgatggg
    11761 caatggaagc aaggaactgc cattttagca caaactggag aattaacagt tgagcaggct
    11821 aatgaaaaga atcttttact ggcaaaagtc gacattatgc gtgataggcc cagagatgcc
    11881 ttagccaagt tagctagaat taaagaacct gaaagactct cattatataa tcaaatccaa
    11941 tttcacgaac aattggcaaa gtcatttcgg tcccttggta gttactctga atcagttagt
    12001 gagcgtatca agttagaatc attgctccct gatgaggaaa gtcaggtcaa taatcggcgg
    12061 gctttgtggt tgaccttaac cagtttacct caagaggagc tgaatacaat ggctctggag
    12121 actgtgaaca aatcagaaat gcaaggctgg ttgcagctgg ctgttatttc ccgcaagtat
    12181 agaaataact caaagtcgtt gctggctgcc ttggatcaat ggcagataca ttttagtaat
    12241 catccagcaa atcagatttt gcctaatccc ttagacagca tatcaggtaa attattagcc
    12301 ccacctaaac aggtcgcttt gttattgcct ttaagtggtc cattatccgg accgggtaat
    12361 gctatacggg aaggctttat ggcagcctat aaggccaaca ggggagatga atcaacaaaa
    12421 attaaagttt atgatacaag caaaggcgat ataacgaaca catatcgcca agctatttct
    12481 gacggtgccg aatatgttgt tggcccttta acgaaaactc aagtcgctac cattgcatca
    12541 atggagcatc cggttcctac cctattgcta aacgatacgg ataccagcac acaaagcaat
    12601 tcttattcct taggcttatc ccctgttaat gaagcgattc aggttgcaat aaaagcaaga
    12661 agcaaaggat ataaaaaagc gctgattatt gcccctaata atgcctgggg taatgaagta
    12721 acgaaggctt ttatcaatca gtggaatgaa gatgggggcc atattgttga ctctcttcgc
    12781 tacgaagcca aacaagattt gaataagagc atgaaagatt ttttacaaat caccaatagc
    12841 caggagcgcg agaaaaaact aaagcaggta ttaggctata atttccaatc gactacgagc
    12901 cgaagacagg attttgatat gatattttta ttggcttatc cttccaaggc ccgccaaatt
    12961 atgcctttat taaaatatta ttatgccgga gatgctcctg tgtacgctac ttccagtgtt
    13021 tatggcggta gcgccaatgc tttgaaggat aaggatttgg atgggatcat tttttgcgat
    13081 atgccttggg tattttctca tcagatggga acgaaaaact ggcctgaaca attcaatagc
    13141 tataacaggc tttatgcttt aggcgtggac agttatacct tggctaccca attaaaccaa
    13201 ctgatattat ttccagctga tggctctaac gattcaacag gtattttata tttaaagccg
    13261 actcaacaag tagccagggt tcttgaatgg gggcaattta aacagggttt agcccattca
    13321 ctaggtgata ctgtttagtt ttgaagtgag ccaagaataa tgacccaaga aaaaggaaag
    13381 tttgctgaac aattggcttt aaattattta aaggaaaatg gtcttgcatt agtaatgcaa
    13441 aactatcatt gccgtttagg agaaattgat ttaattatgc gtgagggatc ttatctggtt
    13501 tttgttgagg tccgttcacg ttcaaatatg aactttggtg gggggcttgc cagcattact
    13561 tacgaaaaga aacaaaaaat aattaaagca acttctcatt atatgattaa atatcgcata
    13621 caagataaat ttcctatacg gtttgatgtg attagtatag atggaaaatc aaacaagatt
    13681 acctggttaa agaatgcatt tgatgcagga tgctaggaag ataatgaata gaaggttttg
    13741 aaatgattca gttagaagaa agagtaaggc atttatttgg tgtcacaata gaagcaaaaa
    13801 tatcagcagc tgatgtgttg tcgggttcaa tagctaaagc agggaagttg ttggttaact
    13861 gcttattgaa tgatggaaaa atattcattt gcgccagtgg gggatccagt gccaattgca
    13921 tgcatttttc cagttccatg ctaaatcatt ttgaagtgga aagaccttct ttgccggtca
    13981 ttaatttatg cgctgatgcg tcctgtttga gttcatttgc cgatgagcat cactttggtc
    14041 aagtgtttgc aaggcaaatt caagccttgg gccaagagca agatgtatta cttttactta
    14101 ccacatcagg taattctgat agcatgctgc aagctcttca cgcagcaagt gaacgcggta
    14161 tggataccat tgcactaagt ggccgcgatg gaggtgtttt ggcgaatcat atggggccag
    14221 aggatataga gataagagtg gcatctgaca gtactgcatt gataagagag ctgcacttgt
    14281 tcatattgca ttgtttttgt gatttaatcg aacaatcttt atttggccaa gttttaggat
    14341 aaaaaatgaa cataaatcta aaatgtaaat ccatcatagt gatgatgatt ggtgctttac
    14401 tgactggttg cgttgctgtg gttgttgcag gtgctgcagc gggaatggtt tatgatagaa
    14461 gaagcgtaac taccatggag gcggacgccc gattatttca tgtgattcat aaaaccatca
    14521 tcaaggaccc tcgatttcgg cactccagaa ttcttgtttc cagttttaac cgggtggtat
    14581 tgctggttgg acaaactccc gccgcatcat tacgcgttct agcagagcgg attgcccaaa
    14641 atgcccctgg agttaaaaga gtgtatgatg aaataaccgt tgataatccc attcccttga
    14701 ctcaacggac aaaagacagt tggattacaa gtcaagtgag aagtaacatg ttgactaaaa
    14761 agggacttga atcgggttca atacggatag tcactgaaaa tggagtagtc tatctgatgg
    14821 gaattgtttc tgacgagcaa gcattgctag ctgttgatgt ggcaagacgt gttgctggag
    14881 tgagaaaagt cgtgaaaata ttccaatata tacgttagca tgtttaaaca cggatgtttt
    14941 atactaatag tctttttttt agccagctgc ttgtcaggct gtctgggggc tttatggact
    15001 ggggcaactc tagcttatga caggcacaat gtatataaaa aattagatga ttataattta
    15061 ataaaagctc ttaatgatgt tttagctgtc aaccggacat ttaaaaactc agagacagta
    15121 ctggatattg ctgtgttcaa cagagatatt ttaatagcag ggcatgttcc aacccaggag
    15181 ttgtacgatg aattacaact acgtctaagc aaagttaaag gatacaggag gttattcaat
    15241 cgcgtaatta tcaataaaat gccatccaac tcaatccagg acagctggat taccacgaaa
    15301 atccgtagcc aaatttttgc agactcttct atcgatccta acgctttcaa ggtggttact
    15361 tctgatcgtg ttgtctattt gatgggagat gttcaggcag aacaagctga aaaagtgatt
    15421 aaaattgcaa gatacacagg tggtgtacag aaagtaataa aacttatgcg gtattatact
    15481 tatcaaacca gtacgaatat ggcctgatca gaatcaggcc aatagctgca cgtccaatcc
    15541 aggaagtggc taacctggga ggataagtat actgtatcaa tggaatcgac ctttatccag
    15601 tcgttttcgt atttttctca aattaaactc gcgtaacagc cctgtagagc catttacaat
    15661 attttcttcg tctttattgt tcgtttgatt gcatggatga gatctgcaat gttgtcgata
    15721 tgaaaggtga tttttattat gcttgtgtga aagcccatcg ctataacgat tgctcatttt
    15781 ttcttgcagc ctggctgctg tatgctgaat tttctctgac attttttaat ccttaggcga
    15841 ggttaaatag taaatcgctt aacatgtgat taatgctaaa ccctacatta caattatagc
    15901 tcgtaatttg aatatcaacg ttgattatta caattgttta atttataatt cgcgccgtga
    15961 aagttaggtg aatatgtgaa caaacagctg aataagcgcg gaatcacgcg attgtggctg
    16021 gcgcagttgg gagttactgt aatcctagct ggcctttgcg caattttatt tggtgtcaat
    16081 gcggccagtt ctgcattgct tggtggactg gtttgtatta taccaaatgc ttactttgct
    16141 attaaactgt ttaagcatca aggcgcgaga gctgcgaggc aaatagttaa tagtttttat
    16201 aagggtgaag cgttaaaaat aatattgtcc atgttcctgt ttgcattagt atttttatta
    16261 tgtaggatca caccggcagc gttttttgca tcatacattt tggttcttat gacgcattgg
    16321 tttgccccat ggattattgt caataaacag aataggccaa aaagtgactg aaatggtatc
    16381 tagcacaaac tatattaagc atcatttaac gtaccttacc tataacgtaa atgatatgac
    16441 attcggttca agtggtggtt tttggacctt gaacctggat acaatcttct tttctatagc
    16501 cttgggtcta attgctctgg caattatgta tttagcggca aggaaagtaa ccactggtgt
    16561 tcctggaaag ttacaaaatt ttgctgagct catgctcgag tttgctgata atcaggttaa
    16621 ggattgtttt catggaaaaa ataacctaat aggcccattg gcattaacta tcttcatgtg
    16681 ggtattcctg atgaatttta tggatatcgt gccggtagat atccttcctg tacttgcgca
    16741 gctagtcggt atacactacc tgaaagttgt accaactaat gatttgaatt taacatttgg
    16801 attgtcactt tccgtatttt ttctaatagt tttttacagt ttaaaaataa aaggcgttaa
    16861 aggttttgtt aaagagctca ccctgcagcc cttcaatcac ccactattca ttccatttaa
    16921 tttattgttg gagttggttg ggctgatagc taaacccatt tccttggcat tacgactttt
    16981 tggtaactta tacgctggcg aattaatatt tattttgata gccttactaa ccttaaatgc
    17041 aacaacgtca tcattaatta gtactatgac actgggtacg gcgcaattta ttcttgcact
    17101 cgcctggtcc attttccata tattagtgat aaccttacaa gcatttattt ttatggtgct
    17161 gactatagtt tatttgagtt tggcccatga agatcattaa ccgatttaat tatcatcatc
    17221 cattgtaaag gggataaaat atgcaagctg ctgaattaat agcacaagtt caaagtatga
    17281 ccgtcattgc ggttgccttg ttgataggtt taggtgcgtt aggcacagcc ataggtttcg
    17341 gattgctagg cggtaaattt ctcgaaggct cagctcgtca accagaaatg gtacctatgt
    17401 tacaagttaa aatgtttatt gtagctggtt tgcttgatgc ggtaaccatg attggggtag
    17461 gtattgcatt attctttacc tttgctaacc ctttcctaag cagcctgggt tcttgataac
    17521 acacaaatag ggcgctatgc gccctcactg ttacaggaga atatactttg gatattaatt
    17581 tgacattaat agttcaaatg ttggtttttg ccgcgtttgt actgttcact atgaaattag
    17641 tttggcctcc actagcaaaa gcacttgaag aaaggcaaga taaaattgct gatggattgg
    17701 ctgctgctga aagagggcgt aaagagttgg agttggctca gcatcgtgta aaagatgagt
    17761 taaaacaagc caaggctcac tctgctgata ttattgataa agcgaataaa cgagcatcag
    17821 aaattattga agcagcaaaa gaggccgcaa aacgcgaagc tcaaatccaa gcaaaactcg
    17881 cgcaagaaca aattgctcag caagtaaatc acgccaaaga agaactgaga aaacaagttg
    17941 caaagttggc tattactggg gcagagaaaa ttttaatgcg agaggtagat gcaaaagcga
    18001 atagcgaact gttagataac ctaattgaag agatttaaaa tgtctgatag taccactata
    18061 gccagaccct atgcaaaagc gatatttgaa catgctttag cagaaaaaaa actgagtgaa
    18121 tggtcagaat atcttacatt actggctcaa gttgttttaa ctcctcaagc tactcagttt
    18181 atcgctaatc ctgcatcaac agatgaacaa caaattgaac ttcttattga gatatgtggt
    18241 tcaaagttca agaaaaatga tgcactaaat aatttaatca agctattaac aaccaataaa
    18301 agattgatgt tgttgccaga aattaaagct ctttatgaag tatatagagc agagcaagaa
    18361 aaaatactgg aggttgatgt agtcagctat tccgaattga ctccagcgca acaacagcgt
    18421 ttgagcgaat ccttaagtca aagattatca cgtaaggttt ctttaaaaat aagtattgat
    18481 ccctccttgc ttggcggagc gctaattcga gcaggcgatt tagttatcga tggttcagtt
    18541 cggggcaagc ttaatatgct tggcaccagc ttggccgctt aatttagagg atagtttcca
    18601 tgtcagaaca agtagcgtta aatccttctg aaatcagtga attaatcaga aagaaaatag
    18661 atcaatttag tgtggtttct gaagcacgaa atgaaggtac tatcgttagt ctgaaagacg
    18721 gtattgtacg actgcatggt cttgccgatg ttatggcagg tgaaatgata gagtttccag
    18781 gaggggtcta cggattggct cttaacctgg agagagattc tgttggtgct gtaatattag
    18841 gcgactcatc cacattagct gaagggcaaa aaggtaaatg tacaggccgt attctggaag
    18901 tcccagttgg aaaaggtctt ttgggacgtg tcgttgatgc tttaggaaat cctatagacg
    18961 ggaaaggccc gatagagtca tctggtatgt ctccaataga aaaagtggct cctggtgtaa
    19021 ttacacgtaa atcagtagat caacctgttc agacaggatt aaaagcaatt gatgcgatga
    19081 tcccagttgg aagaggtcag cgagaactta ttattggtga ccgccaaact ggtaaaactg
    19141 ccattgctat tgatgcgatt atcaatcaaa aaggtaccgg cgttaaatgc gtatatgtag
    19201 ccattggcca aaaggcttca tccgttgcat cgattgttag aaaattggaa gagcatggcg
    19261 ctcttgagca tactattgtt gtggtagcag gagcctctga ttcagcagca ctccaatata
    19321 tagcacctta ctcaggttgt accatgggtg agtatttcat ggagcgtggg gaagatgcat
    19381 tgattgttta cgatgattta actaaacaag catgggcata tagacaaatc tccttgttat
    19441 tacgtcggcc accaggtcgc gaagcttatc ctggtgatat tttctatttg cattctcgct
    19501 tgttggaaag agcagcaaga attaatgccg atgaagtaga aaaattaact aatggggaag
    19561 tgaagggtaa aacaggatca ctgactgcat taccgattat cgaaacacaa gcgggagatg
    19621 tgtcagcctt cgtaccaaca aacgtaatct ctattaccga tgggcaaata ttccttgatg
    19681 ttgacttatt taactcaggt gttagaccag caattaattc cggactgtcc gtttcacggg
    19741 ttggtggtgc tgcacaaacg aaaatcatga aaaagcttgg tggtggtact cggcttgctc
    19801 tggcgcaatt ccgtgagtta gaagcattct cccaatttgc atctgatctt gatgatgcaa
    19861 cacgtaagca gctggagcgt ggacaacgca ttacagagct gatgaaacaa aaacaatact
    19921 ccccacttac agtggctgaa atgggggtaa gcttgtttgt ggtagaaaag ggatacttgg
    19981 acgatgtacc tgtaaatgaa atcagctcgt ttgaagcttc cttgcacgat tacatgcgca
    20041 gtacccatgc cgctttatta catgcaatta atgaagccgg tgcttatgat aatgaaatcg
    20101 aagcaaaact gaaaaaagca gtagaagagt tcaaaaacac aggcagttgg taaggtatgg
    20161 cttcaaagag ctaactcttt gaggcactaa ggccattatt tgaaagtaat ggcctctaaa
    20221 actaattaag gcgaattgaa agatggctgg agcaaaagaa atacgttcaa aaatttcgag
    20281 tattaataaa actcgaaaaa ttactcgagc tatggaaatg gtggctgcaa gcaaaatgcg
    20341 caaaacgcaa gaaagaatgc gcgcttctaa accttatgcg aataaaatct atgaagtcat
    20401 aaagcatatt gctcgagcag catctgagta cagacaccca tttatgagtg agcgcgaaat
    20461 caagcgtata ggtattattg ttgtcaccac agatagaggt ctttgtggtg gtttaaactc
    20521 gaatctattt cgtgaaacaa ttcgtactat tcgtaattgg caagaacatg gcaaggaagt
    20581 tgatatcgct gttatcggaa gaaagggcca agcctttttt aggcgtgtgg gtggtaacat
    20641 attaggttcc atagatcatc tgggtgatac accgagtatt aatgatttca ttggtgttgt
    20701 aaaaataatg cttgatgctt attacaatgg caccattgat tctttgcaca ttgtatacaa
    20761 tgagttcatt aatacaatga cgcaaaaacc ttttgtaaaa caattattgc cgttaccaaa
    20821 atcagaagaa gataaaaaaa cattgggtca tcattgggac tatatttacg agcccgaagc
    20881 taaagaatta ttagatgaaa ttttagagcg atatattgaa ttacaagttt atcaagctgt
    20941 tgtagaaaac attgcttgtg aacaagctgc aaagatgata gcaatgaaaa gtgcaacgga
    21001 taatgctggt gatttgatta aagaatttca attggcttat aacaaagccc gacaagcagc
    21061 cattactcaa gagttggcag aaattgtcgg tggcgcagcc gctttataaa gagggtatat
    21121 aatgagctta ggaactgtgg tagaagtaat tggtgcggta gttgacgtag aatttcctcg
    21181 tgatagcgtg cctaaagtca atgatgcgtt aaagcttgtt gatagtgatc tggtttttga
    21241 agtgcagcag caacttggag acggagttgt gcgtactatt gccatgggaa caaccgatgg
    21301 tttaaagcga ggattaaaag cagaaaatac aggccatcct attcaagtgc cagtaggtaa
    21361 gaaaactttg ggacgcatta tggatgttct tgggcgtcct gtagatgatg ctgggcctat
    21421 cgatgctgaa gagacttggg ctattcatcg taaagcacca agttatgaag agcaagctgg
    21481 cagccaggaa ttattagaaa ctggtattaa agtaattgat ttgctttgcc cttttgccaa
    21541 gggaggtaaa gttggtctat tcggtggtgc cggtgtaggt aaaaccgtta acatgatgga
    21601 attaatacga aacattgcaa ttgagcatag cggttattca gtgtttgcag gggttggtga
    21661 gcgtacccgt gaaggaaacg acttctatca tgagatgaaa gactctaatg tattggataa
    21721 agtatcgctt gtttatggtc agatgaatga gccgccagga aaccgtttgc gtgttgctct
    21781 aaccggtttg accatggctg aaaaattccg ggatgaaggg cgagacgttc ttttgtttat
    21841 cgataatatt tatcgttata ccttggctgg ggttgaagta tctgcgctgt taggccgtat
    21901 gccttctgca gtaggatatc agccgacatt agcagaggaa atgggtatgc tgcaggagcg
    21961 cattacctcc acaaaaacag gttctattac ttccatacaa gctgtatatg tacctgcaga
    22021 cgacttgact gacccatcac cagctacaac ctttgcacac ttggatgcca ctgtagtatt
    22081 gtctcgtcaa attgctgaat taggtattta tcctgcggtt gatccgcttg attctacatc
    22141 acgtcagctg gatccattga ttgtaggtca agagcattac gataccgcac gtcgtgtaca
    22201 gcaaaccttg caacgatata aagaattaaa agatatcatt gcaattttgg gtatggatga
    22261 actgtctgag gaagacaaac gagttgtaac tcgagctcgt aaaatccaaa gattcttatc
    22321 tcagccattt ttcgttgcgg aagtatttac aggttctcct ggtaaatacg tatcgcttaa
    22381 agataccatc aaaggtttcc aaggcattct tgctggtgaa tatgatgatt tgcctgaaca
    22441 ggcattttat atggttggaa gcatcgagga agctgttgca aaagctaaaa ccttatgagg
    22501 caagataata tgagtattac aacgcacttg gatattgtta gtgcagagca tgaaattttc
    22561 tctggtgtgg tggaaatggt agttgcgact ggagaattgg gtgaaattgg aattactcct
    22621 gggcatgctc cattactaac tgtgctaagg ccgggtgaag ttagaattac tttgcaaggt
    22681 ggcacacagg acatatacta tgtgcagggt ggaatgcttg aggttcagcc tcactgcgta
    22741 acaattcttg ctgatgtggc agaacgagcc gagcacctgg atgaagctgc tgcattggca
    22801 gctaaagcga aagctgaagc cgctatagcc agtaaaggag gtgatattga ttactctgtt
    22861 gctgcagcag aattggctag ggctgtcgct caaataagag caattcaaaa gaccaggaaa
    22921 aaaatgaagt aagagtttcc tgtttgtgga aactgctttg ctatgaatat tatatacctc
    22981 tacaaaacgg gtttcttccc gataggaaaa acccgtgctt tactctttta gtaaatataa
    23041 ttttgtcgcc gtatctttct ctctaataca ccagaaaata atgtgctaac tctaatgctt
    23101 tgcattgctc tattatttga aggaattcaa gttatctgcg gatgcataga tatggtatcc
    23161 agtatgtaca ttaccattat ggttgaaagg caataccagg ggcaatggtc atttaaaaat
    23221 caagtggtaa ccgctgaaat ttgaaaattg gtctatagtg gagagatcaa tttataactt
    23281 ataaaaaatt cttggtaatt aacaacaagt tatcttattt attccatatt cattcacata
    23341 gttatccaca ggatacaaag atataaaatt aaatgaaatt aataaatgtt aatataaatc
    23401 aacaagttaa atttaatcca tatagatgaa gcaaaaagaa tataaagtta tacacagata
    23461 atacgatgat tataattcta acagcatgaa tattctgtta aaattgtttt gcattgtctt
    23521 atacgattca ctaggatgat ggatagcaac taatatatga agaaaatacc caatatgaat
    23581 gaagtaactg aaaaaaaacc gcttactgag tttacagaaa aagcttatct tgattattca
    23641 atgtatgtca ttttggacag agcattaccg agcatcgcag atggacttaa gcctgttcaa
    23701 cgcagaattg tttatgctat gtctgagttg ggtttgaaag caacagcaaa atataaaaaa
    23761 tcagcgcgta cagtagggga tgttcttggt aaattccatc ctcatggtga tagtgcatgc
    23821 tatgaggcta tggttttaat ggctcaaccc ttttcatatc gctatccatt tgttgatgga
    23881 caaggaaatt ggggatcccc ggatgatccc aaatcatttg ctgcaatgag atatacagaa
    23941 gcaagattat ctctttatgc tgatgtgtta cttggagaac tgctacaagg aaccgtagat
    24001 tgggtagata attttgatgg tactttgcaa gagcccgctc ttttaccagc gcgtttgcct
    24061 aatattttat tgaatggtgc aacaggtata gctgttggta tggcttcaga tattcttccg
    24121 cacaatttga ctgaagttgc taatgcgtgt attcatctgc tggataaccc ccaagccgat
    24181 attaagacta tatgtgaata tattaaaggc cctgattttc caacagaagc agaaatcatt
    24241 agcccaagtg aaacaattag agctatgtac gagtctggta atggctcaat taaaatgcga
    24301 gcaatttata ctcaagaaaa acaaaacatc gtcataacag ctttgcctta tcaagtatcc
    24361 ggagctaggg taatggagca aatcgcgttg cagatgcaac agaaaaaatt accgatggtt
    24421 gaggatttac gtgatgaatc agatcacgag catccaacac ggttggttat tatccctcgt
    24481 tctaatcgag tagatataga aagtttaatg tcgcatttat ttgctaccac ggatttggag
    24541 cgtagctatc gagttaattt taatatgatt ggacttgatg gcaagccaag ggttaaagga
    24601 ctattagcaa tcttgactga atggttggaa tttaggcttg ccacagtaaa aagacgtctt
    24661 caattcagat tggaaaaggt actggagcgt attcatattc ttgaaggatt gcttatcgcc
    24721 tatcttaata tagatgaagt cattgcaatc atcagagaac atgagcaacc aaaacaagga
    24781 ttaatttcgc gctttaactt aaccgaaaga caggctgaag caattttgga aatgaaactg
    24841 cggcatttag ccaaattgga agagattaaa ttgcgtggtg aactggacga gctgtctgat
    24901 gaacgtgata ctttacaaaa aacgctggct agtcattttc tcttaaaaga actggtaaaa
    24961 gaagagataa cgaaagatcg agatgagttt ggtgacccac gccgttcacc cattattaca
    25021 agggaggact ctcaggcatt aaaagaagag gatattttgc ctaatgagcc tattacggtt
    25081 gtattgtcta aaaatggatg ggtcagagcg gccaaggggc atgatgtcga tggtaaggaa
    25141 ctcagctata aagctggaga tgaattcaaa gcccaaacgc tagctcgtac taatcagcaa
    25201 gttttatttt ttgatggaga gggaaaggtc tattcgcttc caggacatgt attgccatca
    25261 gcacgtggtc aaggcgagcc tttgaccgga aaattaaacc ctgctgaagg agtattgttt
    25321 gaagcaattg ttggcggaga gcctgagcaa aaggtgttat tggctactga ttccgggtat
    25381 ggatttatcg ccaaaattga agatttatat gtaaaaaatc gtaatggaaa ggcatgcatc
    25441 aaattaagtg aaaacagtca tatattgccg ccaaggctaa taccaaatcg agagcaatta
    25501 tttgttgctt gtgcaacgaa tgtagggcgt ttattgattt tttctcttga ggagttgccc
    25561 gaattatctc gaggaaaagg gaataaattg atcagtatcc ctgccgctaa agcggtctca
    25621 cgagaagagt atatagttga tatgcaagtt ttaacaacaa atgattcact gacagtttat
    25681 gccggtaagc ggcattttac tctgaaaggt gaggatttga ctcactataa gggtgaacgg
    25741 ggcaggagag gaaaccggtt gccaagaggt ttacaaaatg tttctcaatt gcatgttact
    25801 caatctgagt aacatggccg ttagggactg tatgtttccc gtgaaacacc tttaagtgat
    25861 gatttgaaat caaacagaat aatgcacaga gctggcttat tgttcaataa ctgcaacgtt
    25921 attctttctc aagagttttt cttaagcttt cttttctatc taatgataaa tctaacaaag
    25981 gcagggtagc taacgccatg tttttataca atctcttgat ttcactatta tctattgctt
    26041 cgagatgcaa ggaaatggtt tgcatatctc ctctagtcaa aggtccggtt aaagcacttt
    26101 ttgcgctatc tacctgattc aagttaagaa tattgctgta catgagatca catatcattt
    26161 ttcttgattg tacttgctgt attcctgctt ttaaaagtaa ctcttcacta tatgaagcta
    26221 aagtcactaa ataattagaa gcaatcgttg ccgcagtatg ataaatgacc ttcatttcag
    26281 gttgaattgc gataatgcag gcttccagct tctcaaaagc aaacctaagc caacgacatg
    26341 cctcataatc tccctctagc acgcaatgga cttgcttaaa cgcattggaa tccaaagaat
    26401 tagtacggaa tgctttcaaa ggatgcacgc tggcaatata acaaccctgt tttttcagag
    26461 gaagaagtac acttgaatta agagctccac tgcagtggat gaccaagctg ccttttttta
    26521 atatctcaat attgtccaat atcgatacta catccaaaat ggagtcgtcc ctacaggtta
    26581 tccacgttat atccgcttca gaaagatcag atattttacc aaccgcattg ccaaatccga
    26641 tttcattgca ggctatcctg gcacttgcta aatttgagtt acaaatagaa tttaaagagg
    26701 caatctgatg aacagacatt gcaatgccta aattcttacc cagacgccca gcaccaataa
    26761 tattaaaatt catagtgaaa ccaatgtctg tctcagtaca ttttcttatt ataatggaaa
    26821 ataatttatt gtcttgctaa attatcagtc tgaattaagc ggacattagc agagtctgag
    26881 gggaatcgtt tgcgataaga agttgagtat aaattgttat tctagatata aaaaaataaa
    26941 attttgtcgt aatttgtatc ttctgattgt ttttttctcg aatttgtagc agcatgaaat
    27001 ttccacaaaa aatcaggatg atattatgaa aaaacggatt gtttcattat ttgtcatttc
    27061 aacagttatt tcatctgtaa acgctgcaac ccaagcaatc gtatggggtg agtcaacgaa
    27121 agcattgcct caatttatgc aagcacatcc ttttaagaaa caaggattat tgcaaactgt
    27181 aaaaagtcag caaagtgatt ataaactgga gttacaaaat aattctaacc atgcgaccag
    27241 gcatgctcgt tatcaaataa cttacaaggg ggttccggtt tggggttatc aggtcatttt
    27301 tcattccaaa gatggcgcca aagaaactgt aacaggcatg aatattaccg gaattgaaca
    27361 ggatattaat tctattgagg gaaaattgag cccgaatgat atagaacaaa agattcttgg
    27421 aacggtaact gaaccagtta aatttaaaaa ccttaaaaag gttatttata tagataaagg
    27481 aaataaagct catttggctt atcatttgtc ttactattcc aatagtgaaa aaaaacatgt
    27541 gaatgcacct aattatctta ttgatgccaa tagtggtgag attttgaaac aatgggatga
    27601 ggtgcgtcat gaaagaatta gtcagggatt aggcggtaat gcgtttactc tcccataccg
    27661 tcagggaatg tttcagcatg gtaatgcttt gcctggcctt ccctcattag ggaagtttga
    27721 tgttaatgtt gaggatggct tatgtcgtgt tgaaaatgaa tccataaaag taatgaattt
    27781 ggaaaatcat aatataggtt atgatttctt tccagttacc atatttgcgg agtctgtact
    27841 gaatttaagt gctttttcct acccatgtaa tgaaaccaat ttgtttttaa actatgctga
    27901 tggcagaaca ggtcctgtca attatgcttt ttctccagtt aacgatacga tgtattttgc
    27961 ccaacaaacg ttagatatgt atcaaaaagt ttatggtgtt aatcgtccaa taggtgatga
    28021 tttacctata cgggcttaca cccatcttgg tgacatggat aacgcttttg cagtaccaac
    28081 tatcagtctt gatggggtag ttcttgcaca tcagcaaatt gtaatcggaa atggtgatga
    28141 gtttttaaca gctcccgccc agagtgtatt aggacatgaa ttatcgcaca attttactgc
    28201 cttgcattcc ggattgatgt atgaagggca atctgggggg atcaatgaat ccttctctga
    28261 tatggcggca attgcattgt tagattatct tagtaaagat tatccatggt attgggatgg
    28321 tgaggattgg accattgggc gtgaagctgt aaaaagtggg caacctattc gttatttgga
    28381 tgatccagcc aaggatggag tgtctatagg gcatgctagt gaatacactg atgcgttaga
    28441 tgtgcatata acgagcggag tatttaataa agcattttat ttattagcac ataaaccagg
    28501 ctggtctata cgaaaagcat ttcaggttat ggttgacgcc aatatgaatt attggtctcc
    28561 tattgcatac tatgattttg ctgcatgtgg cgtcattcag gcaaccatag ataagcattg
    28621 ggataaaaca cctgttatcg aggcatttgc cgaggtggga gtcgtttgtc cgatgcataa
    28681 aagctagttt taaagtggtt tgacttcaat agttcaagct tcttaaaaca acgagaatta
    28741 ctaaggaagg ttgatcattt tctatagaac gcatactgga taagataatt gttccagata
    28801 tttagagctt gctgctgtac tctaaagggt gaggctcagt tcaacaaaga gctgagccgg
    28861 agccatgaga ggataggtag caaaggagca accacatccg agtcaagatt tctctacgtt
    28921 gaggaggtta ttcctctaaa atagagggtg aataaaactc aatttatttt taaaaaaaat
    28981 ccccatttgc tgcaaaattg gttataatat ttacattttg tagttttgat actatttatg
    29041 tatcgttcaa aataaccaaa ttttgatcca aaaaataatc tttattaccc tatctccatg
    29101 aaatagggtt aattagtaaa tgaacaagag gttaacttcc aatgagtatt acagcccagg
    29161 agttggtaaa acaatacaag cttcgtttga ctcccgccat ggaaaacgat ttattgtcgg
    29221 aagaaagcag attaaagaag gagcttgaag cggttccttt taactctgag gaaactctgt
    29281 ataaaagtat tttgcaaatg attattgtat tttatgaaga aaatactttg gaagaaaatc
    29341 gtgatttatt gcaagatcat gagttaataa agcaattaag cgcattgatg tgggatgaca
    29401 tccaaatcaa gttaattcct tttctaattc agaaaaattt cactttaagt gagattaaag
    29461 agctcttatt tgacgaagcg tattatcgtt cgcttcatgt ccttgtcgat tttagtttaa
    29521 ctcaagatat acctgaatta ttagcccatc aggaaaaaag agagcaacta aaattcatta
    29581 atactttagc aaatgatcat tgcagaaagc tatgtcttat attttgggtc aaggggagtt
    29641 tatcgatcaa agaaatacaa gacattgtta atgctaccag ccattaccca atgctggctg
    29701 aaacattgat agcattagat aaaaccaaaa ccatttctat aaaacagcta aagaaactgg
    29761 ctctggatcc caaaaaacat caacaggaat ctattttata tcattactct gaacaattta
    29821 aagcctacaa tttgcgtaaa tccgatttat cccaattaaa tttggatgat ctggatgctt
    29881 taggaaaatc ttttaaagtc ttgaaagaag cggggattgc caatgattat gcttatcgtt
    29941 tggtactaaa aaataataaa acagggcaat tgcttcgctt gtttttgcca gagctcgcta
    30001 aaatcgaatc gctctctcat agaaaagctt taattgaact tttatatata ggagctcaaa
    30061 aaggggttgt tactcaggga aaggcgcttt tacaaatcaa agacagcaat ttattggctt
    30121 tggcacgagc gttgcgcgaa cgatttattt gtgtccagca gatgcaagat ttaggattta
    30181 agaaagagat cattgcattt acaggtgaag aaaacaatat caactccagc cgtttcaggc
    30241 atgtcattat gcgggtagaa gaaaaatgca aggatataca tgaaagatta cgaaaatcat
    30301 ctcttgataa agataaagtt ggaaattggc agcgtgctga tgaaaaatac cggcaaacac
    30361 tctacagcat tgcctatgat ggcataacaa aatcaggtgt tgatctgcac attaaaatga
    30421 aaagtgcgga aaaggaaata ttgagtattg ttgaccctga aataaagtca ataatacata
    30481 aagttttagt cgttatagct aatattatta ttacggcact gaccttgggt tttgctaacg
    30541 atttaaaaga aagtgccact ggcaattatt ggttttttaa ccaaagtcca tctggagaag
    30601 tcataagagc cttaaataag gaggtattaa ctactattga ttctccggag ttaattacaa
    30661 tttcgcccta atcacagggc ttctgtcaac aaacccttat tgctttgggt aatataagac
    30721 aaccttgaat gtttaattaa aatgatgatt tcgagtttgt catcatagca aataaacaag
    30781 caaattttca tttataactc attatgtatg ccagatgtct agtttgtttc tagtcaagtg
    30841 ttattttgta tgaaaattat acttgaactt cttgactatt tctgattaat atggggcata
    30901 ggatggattg atctgattta aaggatttga tatatgggtt tgcccaaaaa agcactgaaa
    30961 gagagccaac tgcagttttt aacagcgggg actgcggttt cggatagctc gcatcaaaca
    31021 tacaaggtca gttttattga aaatggcgtt attaaaaatg ccttttataa aaaactggat
    31081 cctaaaaatc attatccgga acttttagcg aaaataagtg tcgctgtctc tttatttaaa
    31141 aggatttttc agggaagaag atcggccgaa gaacgtcttg tatttgatga tgaagagagg
    31201 ctggtaggta ctttatctat cagtgttgac ggatttaaag ggtttaattt ccataaagag
    31261 tcagtgcctc aagagtcttc cgccaaagaa caggtgattc ccagcacaag aaccttaatt
    31321 gaaaaaagtt tcatggaaat ccttctggga aggtggtttc ttgatgacga tgatggtcac
    31381 ccgcataatc tgagtttggc cggggacatt gattttgata tgttttttta ttggtttacc
    31441 atctacatga aagagcccag gccggctata ggtataccta aaacaagggt taatttgacc
    31501 gtgcgtgatt gggaaggatt tcctaatgtc aaagactcaa agccttttca ttggccaaca
    31561 tataaaaatc cggggcagga aactttgcca acggttttgc ctgttcaaga taagctggtt
    31621 aatttaattc ttgaaaagac gtacccggat cctggtcaat ttgagcaact cgctcatgag
    31681 ccagtggctc aggagcaaaa atttgctgcc gcgttgaaaa tattgttaac ttatcagccg
    31741 gagatgataa gaaaacgttt gaccgagctt tttggtgaga tgacgctaaa ctacacttct
    31801 ctggatgaaa cggatgtcgc cctgcgcagt cagtacgaga aaacatttcc tcatctatgc
    31861 aatgaaaaca ccaatatcaa accattcgtc gatttcataa tgaatttata tcaaatgcat
    31921 tatgataatt tgtatcgagt agtggttttt tatatggggt gtgaaaataa tgggtacgga
    31981 gttccattgc ctgcaacgaa ttcagcgctc taccacaaac cttctttcta taaagacata
    32041 gtcgaatggg caaggactca aaatattacg atttttagca aggacgacag cagcatcaaa
    32101 tttgatgaag atgagttgcg aagaagatat caccaggtgt ggcgagatgc ttatgcgcct
    32161 acattcagag atctgttgca tgactcctat agcctgacta ataaactatt gcagcaagtg
    32221 tctacatttc acgttgttct ggatgaagta gaaggcaaaa agccaaccga tgacacctta
    32281 accaatgcct gggagttgtt tggcaccatg cctgagttat cactcgagaa aattacccca
    32341 ttaattagtg tagataaaga cagcaaacta agaacagcgt taatattgct tgttgaattt
    32401 acaactcaat ttcatgcagt agctaaggct tattatcaga aagaccgaaa ggatttaacg
    32461 gaggaggaca atcttgaatt ctccgagcaa ttggtacagt tgtatacgaa ttataacctg
    32521 aaaattcgtc aaagtttggc acatacaagc actcttgccg gtgaatttaa tcgcatagcc
    32581 gttgggttaa agcaatacac agagcgggcc aatttccaat tgcatttaac gacaacagat
    32641 gagcagatga aggaagcaac tgtagccacg actcccaaag aaatattgcc gcatacgcat
    32701 gaagacgtca ttaggcagtt taatgattct ttattccttt gggccaagaa ctcaagacct
    32761 gaagatttat ctcatcatat ctctgaaata attgataaat attatgcccc gactatagaa
    32821 ctgttatcaa agcgtcatag agcacaacct gttaaagaat atttacaggc tagcgtaaat
    32881 gaaagtggtg aaaacagatt ggcttatatt ttaagcgcag gggagggtga tacaggtgct
    32941 ttaaatactt tgcttattca acacctcacg ccttatatgc tacaaactta tcctttgttg
    33001 agcattcgta atgcagttaa agaaggtggt tttgataagg atttggaaat ttttaccaaa
    33061 gcagctgtag atttcgccaa acatgacagg cgttttattc atttatacaa tgtggagggt
    33121 aagtctctat ttttcaaaac aatgtatgag tggattgatg agctccccgc gactaagttt
    33181 aaggggttgc ttgagtctgc attaaaagat tacgaaggta aattatggtg gtccacttca
    33241 aggagatcgg aggtggaagg ttattgtaca aaattctcac aagcaaaaat tgttgctatg
    33301 acctttttga acgggaaaga ctcctcctct ctgaatgatg ttttatttga taaaatcatc
    33361 gcggctatac aaaaagacat aaacaaaaac aaagaaaaat taaaggtacc aggattcagg
    33421 ctaattaatt gctacaatgc caaagaacac agggctgatt attttaaaga ggtcaaaaat
    33481 tacgcagaac ctgtatctca taggcaagaa actactttga attcaaatat tacgagcttg
    33541 gtggtctaaa ttttctctgg catggagatt tactcgtctt attgtgacaa tacaataagc
    33601 tttaggttat tattctcttt tttcagtttt gagtaaaaag tatgtttcgt gatgtactga
    33661 agacactacc ccaatacctt attccgaaac acggtatcac tgctttagca gggtattttg
    33721 ctgacgttaa gaaccctcgt ctcaagaatt tcctaattcg aaactttatt cgtaagtttg
    33781 atgtcgacat gagtgaggcg cttattgaag atcctaagtc ttatgactgt tttaatgatt
    33841 tctttattcg tcatttaaag ccggaatgcc gtcctctttc tcagtcagat gtgatttgtc
    33901 cagtagatgg ttgtatcagc gaaataggca agattgaacg agggcagtta ttgcaggcta
    33961 aaggcaaata ttattctgta caagagcttt tagcctgtga tgggcaattg gccgaacaat
    34021 ttgtgcaggg acaattcgca acgctatact tatcgccgaa agattaccac agggtacata
    34081 tgccaataga tgcagagctc gtttcaatga cctatattcc aggtgctttg ttttccgttc
    34141 aaccagcaac tacacgtgtt gttcctaaat tgttcgcacg aaatgaaagg cttgccattt
    34201 tttttaaaac gaaaattggt ccgatggtga tggtgatggt tggggctact attgtgggcg
    34261 caattggaac cagttggcat ggtgatgtca aaagatcaaa aaaattagaa cgctttgatt
    34321 atagtgagca gttttcggac aaaattatat ctcagggaag tgaaatgggt tattttaaat
    34381 tgggatctac ggtcgtttta ttgtttgcaa atggagaaaa aatacaatgg gataaagaat
    34441 tattggctgg cagcaagatt cagttgggta aaccaatggc aattattact taattaatgg
    34501 taccgagaag aggactcgaa cctccacggg gttgccccca ctagcacctg aagctagcgt
    34561 gtctaccaat tccaccacct cggcataggg cgttaagtta cgcaaattat agtgttctgt
    34621 caatacacaa ttgcgcgaaa tcaaaaaagt ataaattact tgagatttat tgctgcgaat
    34681 aggggaaccc ggcttttaat attctttctc gtactccctg ccattcactc gtattgggtg
    34741 gtagctcgat agcgatacaa atggcatcgg atgtatcagc taatcttagt tgataataca
    34801 aatcataggc cgatttttca ggatgcattc ccagtggatg aaagagatta gtctcgacac
    34861 cagggggttg tctattcgct atgacataaa cagtcccttt gcgtttttga caaaattcgg
    34921 aaagggtttc atattgatca aaataatata aagttttttg tggttgataa tggctatcca
    34981 atttaccagg gacacgaata tcattttttt gagtcgagca acctatggca atcgtttcag
    35041 caatagtttt ttcatctatt atcccatggc gtaaaatttg ataagaatca ggattggtcg
    35101 catctacaat ggttgattca attcccacac gacagcgtcc tccgtctaat atagttaatt
    35161 cggcatcatt aaatgattgt ttaacgtgca aagcggtggt cggactaatt tttccaaaag
    35221 gattggcaga gggtgctacc acaggaatat ttaatttttg taacaatttt tgtgtcaagg
    35281 gatgatccgg gcaccttatc gctatggtag attgaccacc agtaatgagc ggattgattt
    35341 gatctttttt acaatggaat accagggtca atggtccagg ccaaaacttg tcagctaatt
    35401 gatgcgcata ctcaggtacc tcttccacga ggtcatgcag atcccaattg gaagcaatat
    35461 gaacaatcag aggatgattt aaaggcctgc ttttcatttc aaacacagct ctgatggcag
    35521 cattattgtt tattggtgct gccaatccgt aaaccgtttc agtcggtata gctactggtt
    35581 ttccttgctg taaatcatga attgcccgat tgagattggt agtaattatg gacatataag
    35641 gggctcgata aaaaatagtt atgtttcagg gtctttctat ctgtttttaa gctcaattta
    35701 ataataagaa taagcttcta taaataaact ggagggaatc aggcaggatc aataacctga
    35761 ttttccctca accctatgct gtaaattcta ttgcgataaa ctaataaatt ttagtctttc
    35821 taaaaaatta aaacttaaat tccagcaatt tctacatcgg tttattatag aacatatggt
    35881 tcatattgtg gagtccacat cgttcttttt atagactcat cgattttatt attgttcatt
    35941 ggctctatat gaccctcctt tataccctgt aaaattaccg ctctcgctat atgttgactg
    36001 acttctctaa tggaatttaa ttctgggagt aagcgtcctt ctccggttct gattgcaggt
    36061 gctagctcac ttaatgcaac tgctgctgcc atcatcatta aatcagtcac tcttttggct
    36121 tgtcctgcaa ctactccaag accaactccc gggaaaatgt aagaattatt gcattgagct
    36181 atctcaattt tatgaccatt aattacaact ggctcaaagg gactgcctgt tgcaattaaa
    36241 gctttaccag ctgtccaatt taacagatct tgcgggattg cttctgcacg agacgtagga
    36301 tttgataaag gaaaaattat ggggcgttca caatagctta gcatggtctt gatcattgct
    36361 tccttaaatt ggttaggttg gccagacacc cctaggagta tagtcggttg ggcattgtta
    36421 ataacgtctg ttaacgtgat ttctcctttt ttttctagtt tccaattttg gagactggta
    36481 gaagaacgga caaatccttt ttgaaatggc aatagatctg tcatctcatc atgtaaaagt
    36541 ccatacctgt caactaaata aaaacgtgat ctcgcttctt cttcagaaag tccctggttc
    36601 atcatggcat gcaccagttg ctcactgatc ccacaacccg ctgatcctgc gcctaataaa
    36661 gcaactcgat gttcctttaa aggagtatta gtaacacgag ttgccgctag tatggcggct
    36721 acagcaacgg aagcagttcc ttgaatatca tcattaaaag tacaaagttg atttttatat
    36781 cgctccaata aaggataggc atgttgttgt gcaaaatctt cgaattgtaa tagcacatga
    36841 ggcatatggc gttttatact ttgcacaaat tgatcaacaa aatcatcata ctctttgcct
    36901 gaaatccgag cgtgtctcca cccaatatat tccggatcgt ccagacgctc cttattattg
    36961 gttcctacat ccagaataat aggtaaagta ttagacggat gtataccacc gcaactggta
    37021 taaagagaca atttgcctat aggaattccc aatccacctg ccccttgatc tcccaacccc
    37081 aagatccgtt cgccatccgt gacaacaatt actttgattg aacgagtcga cgcaatgttt
    37141 tgtatgatgc tgtccagttt gtctcgctct ggataactta gaaatactcc tcttggttgc
    37201 cgataaatgt gggaaaacat ttcacaggct tggccgacaa ctggcgtgta tataatgggc
    37261 aaaatatcaa ccagattatc aattataaaa cgataaaaga gtacttcatt tcggtcctgc
    37321 aaggcacgta agtaaatgtg tttttccaaa gggttttctt tggcactata ggcgtcaagg
    37381 caacgaatta cttgttgttc aagggtttcc actgttgttg gtattaaacc atgcaaggca
    37441 aattcatctc tttcctggag gctaaatgcg gtgcctttat ttagtatggg gtctcttagt
    37501 aagtccattc ccctttttgt tactttgatt gccattagaa cactccttgt gaatatttgt
    37561 agccaagaaa tttaaatcag gatttctaaa taacttcaat tcaactcatt gattttaaga
    37621 tggaagttat ttacaaattt tataaatgct aatttattgt tgtattgtaa ttctcacata
    37681 gtttctagtt tagtcgattt ttgcgatttt gtgctaatag cgtattttgc cttttatttt
    37741 atgccggcat aaaatgatta aaattggact attaggataa ttggttttgt tactcaaatg
    37801 catagaaaaa atagttgatg ggtatttcgc tgtaattccc aggcacacac cggaattcga
    37861 aaatatcaac ggtgcgttaa taatcgcgca tagaggagct cataataatg caagagggat
    37921 aattgaaaac actctggaag catttcagaa cgcaaaagag accggatgtt ggggaattga
    37981 actggatgta catctcacta aggacaaggt gttcgttgtt aatcatgatc ctaccttaaa
    38041 tcgattatgg aacaaggaca ttgctattgc tgatctgaat tttagtgaat tacgggcatt
    38101 ggagtgcaga atcccgtcgc tctctgaaat agtgagtcag tatgggaatt ccatgcattt
    38161 atttattgaa ttaaaaacag ctattgagga ggaagagata ttagtgcaaa ccctgcaaca
    38221 tttaacgcca tgtaaagatt ttcatttatt gactttgcag gaatctacct ttaaaaaatt
    38281 gtgttctttt cctaggcaat ctttattgct tgttgcagtg cataataacg ttaagaaatt
    38341 ttgtgaatta agcatcaagg aggggtatgg aggcgtactg ggtaattatt tattattgac
    38401 taatagggca ttaaaatatt tacaagatgc caatcaacat tttggcgtgg gatttgttga
    38461 ttccaaaaac agtctgtata gagaattaaa tagaggggtt aaatggctct ttactaatga
    38521 ggcaataaac attaatcagt atttaaatga cttaaaaaat agcatgtaat cattttgaca
    38581 atactcctgc ttgtttttga caattcaata atcatcacat agtcttaaat taacagttcc
    38641 attttatcat gtagcaaggg taacttaaag atagcaggtt taaggtcagg acattttcgt
    38701 gagtgacggc agggggtaca atgactctaa aaattacttt tttaggttcc ggatcatcct
    38761 ttgctattgg gccagaaaat tatcaatcca atgtgctttt agagctagaa aacgatacgt
    38821 tgctaatcga tgcaggaaca gatattaaat actcattgcg agatcaaggc ttgacttata
    38881 aagatataaa aaatgtttat attagtcact tgcattatga tcattgtgga ggattggaat
    38941 ggttcgctct aataacctat tttgatccta atcactcaca caaaccttgt ctaatcgcct
    39001 ctgataaaat tattaacgag ttatgggata aaagtttatc cggtggttta agcactttac
    39061 cccatgagag agcaaatctt agcgcatttt ttgatgttaa accggtaaaa caatatgaag
    39121 gttttgtttg gcaatctata aagtttaaat tggtacaaac agtccattat tatagcgaat
    39181 atgagttaat gccaagtttt ggtttgatat ttaactataa taaatcacgc atccttttta
    39241 catcagacac acaaagttgt ccggtacagt taatatcctt ttatgaggaa tcagatatta
    39301 tttttcatga ttgtgaaact acagaatcta aaagcggggt acatgcgcat tattcagagc
    39361 ttgttcaact cccccctcat ttaaagaaaa aaatgtggct atatcattat aatccaggga
    39421 aattacctaa tgccaaaaag gatggtttct taggttttgt tgccaagggg caaagctttg
    39481 tgttttgatt taacttgtgc acatgctccc ggataagccg gggagcatgt tgtcttctca
    39541 ctactgattg gtgaactggt taatagtacg agttattcga tcgaccaatt catcaatttc
    39601 atcttcactg ataataagtg gaggtaataa tcttactact gtttcagctg taatattgaa
    39661 caagacttgg ttttccaaac ccaggacgcg catgtcattt gcaggtctgt ccaactctat
    39721 tcctatcata taacctttac cacgtatagc ttttacatgg ggatgctctc ccaggttgct
    39781 aattaattta tccattaaca aagcgctgtt tttagtgacg cgctcacata gcttatctcg
    39841 ttcaatgacc tccagaacag tcaaagccgt tgcacaagct aaagggttgc caccaaaagt
    39901 cgagccatga tttccaggtt taaataaatc tttagctcgc ttgctcatta aacaagcccc
    39961 aattggcatg ccgtttccaa gtccttttgc cgtagtaaga atgtcaggtt gtatgtcgta
    40021 atgcatacaa gaataaagct tacctgttct gccatttcct gtttgtattt catccaaaat
    40081 taacatccag tcattttgct cgcaaaggtt agccaatgca cgcagataac cttcctctgc
    40141 gggataaatt cccccctcgc cctgaatcgg ttctaccata actgcaacca catcctctct
    40201 gttggccgca atagtatgaa tagcatcaag atcattgaat ggagcacgaa taaacccagg
    40261 gaccaagggt tcaaaaccag cttgaacttt acgactgcct gaggcagtta aggtggccat
    40321 agtgcgacca tgaaatgctt tttccattac aataatagaa ggcgtttcaa ttcccttttt
    40381 atgaccaaac agacgagtaa gtttgatggc tgcttcatta gcttctgccc cagaattagc
    40441 aaaaaaaacc tgctccattc gtgccattga agtgagtttg gcggctaata attcttgttc
    40501 tttaatatga aagacattag aagtatgtat tagcctggct gcctgctcct gtatggtacg
    40561 tgtgacgtca gggtgtgcat gacctaatcc acaaactgct atcccgctta agccatcgag
    40621 gtattttttc ccccgctcat catacaacca cacgccttct ccgtgcgtga aggttacagg
    40681 cattgggctg taacttgtaa ttaaactcat gatgtctcca taaacataaa attatccggt
    40741 aggattgcaa caaatccagc ttgttatttt atttcagatt actgctcgca aaatcccaat
    40801 taactaatga ccaaaaggct tcaatataat ccgggcgcct atttctataa tcgatgtaat
    40861 aagcgtgctc ccatacgtca caagttaata aggcatttaa tccttcagtc attggtgttc
    40921 cagcattgct tgtgctgata atttttaaag cccctgactg atcctggact agccatgccc
    40981 atccggaacc gaatgttgta gcagctgttt gtgaaaattg ttctttgaat gctgcaaaag
    41041 aaccaaagct tttattaatg acttccgcca gcctgccttt tggctcacct ccaccattag
    41101 ggctcatgct gtgccaataa aaggtgtgat tccaaacctg agcggcattg ttaaaaatac
    41161 cacctttagc tttcatgatg atctcttcca gagtcatgga ttcaaattct gttccgggaa
    41221 ttaatttatt taagttggta acgtaagtgt tatggtgttt accataatgg tattccaagg
    41281 tttctttgga aacatgaggg gccagtgcat ccaatgcata aggtaattgc ggcagggtaa
    41341 aagtcatatc aatctccagg attaaaagtc tttagtttat caggataagt gtgatattca
    41401 atgtgccatt actgagcgat tcaatatttt tatagtcttg gccatggata catttctgct
    41461 ggtataataa tcttttacga aatagaggat tttacttttc atcaaggtgg ggatttttgg
    41521 tgcatttagt gctaaattgg tattttattg agcgttgagg tggggtaaaa tatcactata
    41581 agaaaaagat tgataggatt atcacaatag aatgattagt tgcgaccttg tttggttttc
    41641 gacataattg ggttttttat tttataaaag gtgcatagtg gatactttag aaaagattaa
    41701 aaagcaaatt gctgaaaatg cgataatgct ctacatgaaa ggcactccca agatgcctca
    41761 atgcgggttt tctgctcgtg ccgttcagtg tattgaggct tgtggagtag actttgccta
    41821 tgtcgatatc cttgctaatc cggatattcg tcaagtttta ccacaatttt ctgactggcc
    41881 tacctttcct caactttatg tgaagggtga gttaattggg ggatctgata tcatagccga
    41941 aatgtttcag caaggagagt tagagcctat gttgcgtgat gctgtagcgg cataatttta
    42001 ataacaattg tgggatttac ttacccaccg ctaatggaga ttatagaatg agtgttttag
    42061 ttgggcgtaa agcacctgat tttactgtgg ctgcagtaat gggcaatggg gaaattgttg
    42121 ataaattcaa tttacacgaa catttgaaag gcaaatacgg attagttttc ttttatcccc
    42181 tggattttac ttttgtatgt ccttccgagt taattgctct tgatcatcgt atcgaagagt
    42241 tcaaaagacg taatgttgag gtggttgctg tatctattga ttcacatttt acccataatg
    42301 cctataggaa tactcctgtg aaaaatggcg gaatagggcc agtgagattt actttggcag
    42361 ctgacatgac tcatagtatt tgccaatcct atggtgtaga acatccagtt gctggcgtgg
    42421 ctttccgagg cgcttttgtt atcgatacga atggtatggt tcgttcgcaa atcgtcaatg
    42481 acttgccgat aggaagaaat attgatgaga ttcttcgtat catagatgca gtccaattct
    42541 ttgaagaaaa tggtgaagtg tgccctgctg gctggcaaaa aggacaagct ggtatgaaag
    42601 cgtctcctaa aggtgttgca gaatacctgt ctgagcattc cgagtccttg taatataaga
    42661 caaggaaata gaatcttttt gttgttgtgt tttattgaga agcataacac aagaaaattt
    42721 ttatcaaact cctggattac agttttaggt aatccaggct atttatgttt ttctgatatc
    42781 agatgtttag cattcgccac ttgtttatca taaggcaaaa caaatcagct gttttttcgg
    42841 catcataaat ggcagagtgc gcttcctggc tgtcaaaatg taatccagct gcttgcaccg
    42901 ctttggccaa tacggtttct ccaaaaatca aacccgctaa tgttgctgta tcaaagcaag
    42961 tgaaggcatg gaatggtgat tttatcccgg ttcttttgat tgccgctttg atgaataaca
    43021 aatcaaacca ggcattatgc cctactaaaa cagctctttg gcaacgggtt ttttttagca
    43081 agtcatggat gggtttgaat aatcgttcaa gggccgtttg ttcatctacc gcaaaacgaa
    43141 gaggctggta aggatctatt tggttgaatt ccagtgattt ttgatccagt tctgcacccg
    43201 ggaaaggtaa aatatgttca aaataactac tctccttttt taacaagcct tgctcatcca
    43261 tatccaataa gacaatacac atttccagga gagcattttt ctgagggtct attcccgcag
    43321 tttctacatc aacaacaaca ggtaaaaagc cacgaaagcg atttttaatg gtgttactta
    43381 gaatattatt gcgagaaacc attaatactc cagtttatag tgtctccagc agcaataggc
    43441 acaacatgag ttgaaccaaa tggcattgag ttggggatag tttgaggtga tttaatgagt
    43501 tccaattgct gttgattaat agggagttgg taaaactctg cgccaaatcg actaagaaaa
    43561 tcattaagtt tttttaattg gttaagttca tcaaagatct gcgtgtagag cgctaccgca
    43621 tatggagctg aataaatacc ggcgcatcca catgcatttt ccttggcatt gatggcatga
    43681 ggggcactgt cagtgccggc aaaaaatttt ggatttccgc tcgttgctgc aacttgtaag
    43741 gccttttggt ctttttcgtg ttttaaaata ggcaaacaat aataatgagg tctaatgccc
    43801 cctgcgagta gcttattgcg attataaagt aaatggtgtg gagttattgt tgctgtaagg
    43861 ttagcaggtc cttctgaaac ataatccacg gcagctttag ttgatatatg ctccagaacg
    43921 attcttaatt tggggaaatg tttggcaatg ggcttcaggt attcatcaat aaatagtgct
    43981 tcgcgttcga aaatatcact atgagtgact tcgccatgaa tttgcaagac taaattactc
    44041 gtttgcaatg tttcaaaaag ggggtagagt gctttcaaag atttagcacc ctcttcagaa
    44101 tttgtcgttg ctccagctgg ataaagtttt gctccaacta tataagaaag agaggcggct
    44161 tgctgtaatt cttctggttt aacggactca tttaaataaa aggtcatata aggaataaag
    44221 gagttttgtt tgggaatggc cgaaatgatt ctgtttctat aactctcaat tgcgggtaaa
    44281 ttagttagtg ctggtttgag attgggcatc actaacgctc tggcaaaatg ttgggcagac
    44341 gcctggacgg tatgagttag aaactctcca tcccggagat ggagatgcca atcgtcaggg
    44401 cgattaatta tcagagtttg cataataaag gttcctgcat tattgccgat aaaagagaat
    44461 aacatgaatt aggcaattgg gatgagcaaa aaatgatttc agatcatttt atatgcaaga
    44521 aagcaaaagt tattgcaatg agttctttgt tcattactac tgtcgtcttt gctgctccct
    44581 ctatccaata tgcaagccct atgggagaag aaaattggcg tatgacaggc aatcctcttc
    44641 gatgtggctt gtcattgacg attccaaatt atggtattgg ctactttgaa caatacgcta
    44701 cccaatcccc tcacttcatt ctgcgtaaat gggatcaagt tcaacgggcc ttacctgctc
    44761 aagttgtagc aagcccccca gtgtggaagc cattattgaa agggtcctat ctggtaaccc
    44821 gcacttatat aaaacctggg gagtatggac tatttttaaa tcgacagccc gctttaaaat
    44881 tattgacgtt tttatcgcaa ggatatcagg caaattttag ctatttatca gaggaaggat
    44941 ttcgcactac ggttatactc tcacctgttc ggtttcaaaa agtgtactcg cagtatcaac
    45001 gttgtcttgg tggtctgttg cccttcaact atgaggatgt aaaagaaagc gtttttcatt
    45061 ttggggtgga tagtaagtta ttgtctgatg ctgataaagt gcaattacga cggattgccc
    45121 aatacgtgga tgctgatgta caaattgatg tcattaagat tattggtcat gctgacgata
    45181 gtggccgtaa aggttacaat aatgccattt cacaatatcg agcagagtct gtgaaaaatt
    45241 atttgattag gcttggagta cctcaacgaa aactatcaat aacatgggta ggagctctac
    45301 aaccgatagc taggaatgat actgacgagg ggagagctgc taacagacgt gttgttgtga
    45361 cattgataaa aaaataaatt ttgtgattaa aaagtatctt tttatgatgg catttcatcc
    45421 tcgaaaatcc ccttcttggt caacaaattt catttgaaag agacatggca ataatgttaa
    45481 aaaaatagag atactgttca aatttagtta tatcgtcatg aaaatattgg agtttttaat
    45541 aaattttctt gacttattta aaatcactgc agatactcat atcgttgcat gcttcgaacc
    45601 taagcgacga tgcgtgctat tgacaactcc aaatggtgag ttatcaataa aatggaaact
    45661 caataataaa caagtggaga caaggcatgt ttagtttgaa aaaaacagca gtggcagtac
    45721 tcgccttagg aagcggtgca gtgtttgctg gaac
//
LOCUS       DBNCWK010000014        45030 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.014, whole genome shotgun sequence.
ACCESSION   DBNCWK010000014 DBNCWK010000000
VERSION     DBNCWK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 45030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45030
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.014"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..215)
                     /locus_tag="AC06IO_000650"
     CDS             complement(<1..215)
                     /locus_tag="AC06IO_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873863.1"
                     /translation="MIPRLTSSTATPRKIRLFLQQLEKIPDFHGEIDEALSSRIVLST
                     DNSVYQIIPEAIIFPKSENDISHFFKLA"
     gene            370..909
                     /locus_tag="AC06IO_000651"
     CDS             370..909
                     /locus_tag="AC06IO_000651"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIO9873864.1"
                     /translation="MNKITEDSLTEEQKYMVQVWENHTYYEFDTKNVADTMKTMADNP
                     YVNNIPTMTGGFGKQEVKQFYSQLFIPQKPDDTEVELISRTVGAHQIVDELIFKFTHN
                     IRMDWILNGIEPTGKRVEIPLIAIVRFHNRKIISEHIYWDQASVLVQLGLLNSDKLPV
                     HGVEATNKIRELISRSLTK"
     gene            961..1809
                     /locus_tag="AC06IO_000652"
     CDS             961..1809
                     /locus_tag="AC06IO_000652"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9873865.1"
                     /translation="MIKDYLIETMNQDEVNLAITWAAREGWNPGIHDATCFYQTDPHG
                     FFAGKLNGKTIAIGSAVIYDEFFAFCGFYIVDKPYREQGFGLELTKARLAYIGNRNAG
                     IDGVINMLDKYARIGYQLAHNNARYCLNKTIKPISSEPHIVPLNQIDFSQLSSYDRLH
                     FPAPRPQFLKCWISQPQSLALAYLIDGQIKGYGVIRTCHKGFKIGPLFADTPEIAHQL
                     FTNLVPYAKGQLIFLDIPENNPLAIELVSQHQMEKVFATARMYLKGEPLVKKESIYGI
                     TTFELG"
     gene            1813..2703
                     /locus_tag="AC06IO_000653"
     CDS             1813..2703
                     /locus_tag="AC06IO_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIO9873866.1"
                     /translation="MTRDLTGYGSEQVNLTWPNKAKIAINFVINYEEGAELTPLNGDT
                     KAEFYGSDFPFVPKGNAERNYSIESIYEYGSRVGIWRLIRLFDKHSIPITFFITGYAL
                     ILNPEFSGYLASHNHEVAGHGWRWIDYSKESKATEKKHISRCIDTLQELTGQRPLGWY
                     TGRRSPFTRDLLTQIGGFVYDSDSYADELPYFINKHLVIPYSLDCNDFRFTISPGFVT
                     AQDFYQRLKNTFDYLYQENRLSIMTIGLHPRLSGKPDRCHALNEFLSYIRQFQNIWIA
                     KRIDIALYWLTKHTQIKQDL"
     gene            2919..4133
                     /locus_tag="AC06IO_000654"
     CDS             2919..4133
                     /locus_tag="AC06IO_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9873867.1"
                     /translation="MSHLLDLSILKKNRDFTLLYLGQFISFIGTMITSVALPYQIYHL
                     TESTMMVGLLSLAQLLPLLITALLGGVFADRYSRRGLLIISELLLAVGCLFLAFNSKL
                     VNPSVSLIFIIASLMSAITGLHRPAFDSVIQQIVKPEDYKKVGALGSFKFSFCMIIGP
                     AAAGLIIAQYGIVITYLIDLLTFIISLLNLSLMHTIPKPLAKEHPSIIVSLKEGIDFA
                     LGRQELMGSYFVDFFAMVFAMPNALLPAIAQQFGGAKTLGLLYAAPAIGSLIISFVSG
                     WTAKITHDGKAIAIAAALWGAAIIGFGLTTSLWLSLLFLALAGAFDAISGIFRSSLWN
                     HTIPQELRGRLSGIEMISYLSGPRLGDTRAGFVAAGLGIPAALISGGILCIIGVTVCC
                     YALPKFWNYHSK"
     gene            complement(4146..5462)
                     /locus_tag="AC06IO_000655"
     CDS             complement(4146..5462)
                     /locus_tag="AC06IO_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1639 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873868.1"
                     /translation="MAGIYLKYYVPVNQIGENMMINPIEYHPKGDKVNSDYFNEYKTK
                     IYERRLSSGLEDLFGDMCGVVVQVQTGDAIPYIKELYSMTPYRYSRSYISDTHKIYCL
                     LNTKNSPAFLVLEPLDPNFKDDITRLNKMYPNSRAKFNARYVGEIYKCKDKDETRNIL
                     VSHDFNFHNPDMTENKFYCNKHIHFTRLSDFTYNCAGYTDDNIYDFDCLELGQRFYLT
                     KEQEAMLDAKDQESHDNGIKDLIKGIDHMATRILAGEREDAILEFLCLSKYYFWGAYN
                     IYDMNSSTNVNRNPHGHDIKSPAKVFTANNTPFMVNSFENLPMPTETFVRNYGRRMHH
                     IAYEVKDGDHSSGKKNIDFVVETLRDKLNIEFLAKVFGACEDSPDLKQIFSKHSMYSI
                     LITEYVERCHNFDGFFTKENVAALTEAASLDETTKQQHKKASIIGD"
     gene            5553..6110
                     /locus_tag="AC06IO_000656"
     CDS             5553..6110
                     /locus_tag="AC06IO_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIO9873869.1"
                     /translation="MEALIVSLGVITLAEIGDKTQLLAFLLAAQFKKPLPIILGILTA
                     TLINHSLAGLIGIWITTLLKPNVLRWILGISFILMAIWTLIPDEIEQDEKKISKYLGV
                     FGATFITFFLSETGDKTQIATIALAAHYNSPILVIVGSTLGMLLADLPAVYFGNLFSH
                     KIPMKIIHAVAACAFLIIGLITILF"
     gene            complement(6128..8098)
                     /locus_tag="AC06IO_000657"
     CDS             complement(6128..8098)
                     /locus_tag="AC06IO_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIO9873870.1"
                     /translation="MKGVCLGLIICLVVFHDGWAGKRLPELDDLYRIQDISEPDLSPD
                     GKWVAYTVSQLNAKDNISTTEVWKVSVDSKSSQQITFAEDSSSSLPKWSPNGKWLAYL
                     SDGGKEGTTQIWIASVQDNKEHQLTYIDGEVSDFTWSPDSNRIAFIAQKSDPSNEEEQ
                     PIVVDRYQFKVDGVGYLGKGRDHLYLVELKSKAITQLTDGNHDEYLPAWSPDGKYIAF
                     VTKRGKDPDRHFNYDIYLIEPKSGAKERQLTHFPGSDMNPDYDSNLSWSPDSSKIAYL
                     RSNEHQWTYYSPNQLAIIDINTKDEKIIAPRDNWYYKPKWSQDGKFIYALIEESRNTY
                     LNRIDIHSAQIKKLTGGLRYDMDYSLAKGKIILLTTDDTHPSELFILNSGLIPLTQHN
                     KKLLDEVKFQPAEDIQCNSFDGTRVDGLLMKPAEYQSGILLPGFVYLHGGPVYQFSHE
                     FNFDMQWIAANNYVVIAPNPRGSSGRGFHYAKAIFADWGNLDVKDILASVDYVIGKGM
                     VDPNRLAIGGWSYGGMLTNYVIATDSRFKAAISGAGAANILAGYGVDQYTPEYELELG
                     KPWTNPELYLKLSYPFLKANNIKTPTLFLCSGLDFNVPCVGSEQLYQALKSLDVPTQL
                     VIYPNEYHTLEKPSFVIDRLKRYTNWLDAYVK"
     gene            complement(8308..9561)
                     /gene="sidB"
                     /locus_tag="AC06IO_000658"
     CDS             complement(8308..9561)
                     /gene="sidB"
                     /locus_tag="AC06IO_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidB"
                     /protein_id="HIO9873871.1"
                     /translation="MVKIYNAPKPKYSGWEWFKFIAIRTVFPPVLLWDLIKIGANKLL
                     GEWVSGLVLPAQNENFDDLAVSDDTVSNYNEDELICEKHDVITHDGAHLDTFEVRHRS
                     QESIDPKYQKYIINLVGNGMCYEHIIDDIKEDSKALKANVIGFNLRGVGQSTGKAKSS
                     EDLVADGIAQVQRLLDQGVSPQNITLKGHSLGAGVASLVAQYFHQLGQPINLFNSRSF
                     STITNFLVGHMRLERDEIGRAIGHKDSTVGTILGWLAKPFIKLGVALAKWEINAGSAF
                     KSVPEAYKDYIVVRSRKEIRGERIDDAVIPHYASIHKELSSERHKKKAEIDEEIANLD
                     DIIRKADPLAKPGLANARDALVQAREKIKSDRKMETDVQYANGHNSDWNALHNRSGKS
                     AQTFFREFVQRTEADHAVKSIPEIN"
     gene            complement(9812..11080)
                     /gene="thrC"
                     /locus_tag="AC06IO_000659"
     CDS             complement(9812..11080)
                     /gene="thrC"
                     /locus_tag="AC06IO_000659"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIO9873872.1"
                     /translation="MKIRIKSTRGQCVSTIDEAILSGLAWDGGLFVPDRFPDLTIKKF
                     YEYGNLIDFSAHLLSPFFSDSKIDVNNAFFQKFLTFSFPLHHLFKKSYVLELFHGPTL
                     SFKDLGTQFFSECLLHLIENKSAKVLVATSGDTGAAIAHALHGKRDLEGIILFPKDKL
                     TFRQQSQITCWGENIRALAVNGSFDQCQQLVKLVFAKREHKGILTTANSMNIVRLLPQ
                     IIFYAFTSIQLALRHNYSVNFIVPGGNLGNVTACYWAKMLGFPVEQILIANNTNSALG
                     EFLLTGAYQAKSVTKTLATAMDVGEPSNLERLFVLFNNYSECKKQMNAESVSDEQIKQ
                     AILHCYNKYHYIICPHTATAYHRLSTINQDKPWVIVAPGHPAKFEEVLEPLLKKEIPV
                     PEQLKILLGRKQHYSIIEPNYHSLYHFLVE"
     gene            complement(11253..12017)
                     /locus_tag="AC06IO_000660"
     CDS             complement(11253..12017)
                     /locus_tag="AC06IO_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIO9873873.1"
                     /translation="MYLGFPVQSILLFLLFSFTPLFVLAAEKTPLNFFLRPDTYFGFS
                     VIRDTAHYNFKRNETSQELNFTDFDHFDWHGSGLGGEIYLGYEKFFRTHYYIGLEGFF
                     NRSSNEGKIYFFDSNELYSRILKGSFKQKWQSGLAIRPGIYLPSVGIIYGRVGWIVSD
                     VNLSGSITQSGPFGSFSGRFSNTKKKNGVQLGTGLELELTKNWRCRVEWDWNRLQDFI
                     FDSQGRDSNNHRYQTTRIAKHPILEQFKLGLNWRFA"
     gene            complement(12254..12829)
                     /locus_tag="AC06IO_000661"
     CDS             complement(12254..12829)
                     /locus_tag="AC06IO_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIO9873874.1"
                     /translation="MNVYIKSSLWVLIFFVNTLIYASKLPEWEIVPAQSEITFTGTQN
                     GAPVTGRFKKFTGQIFADPSNYKTGSIDIIIDMNSIAAPFEDIVTTLASPDWFNVKTF
                     PNAEFKATKFNQLDDKTYEAEGTLTIRDKSAPVTLKFVVEQISNDQALVEGSSTIKRS
                     TFGVGQGEWASTDEIQDEVIVRFKITATRKK"
     gene            complement(12829..13359)
                     /locus_tag="AC06IO_000662"
     CDS             complement(12829..13359)
                     /locus_tag="AC06IO_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIO9873875.1"
                     /translation="MQLKNTSTSFGLVAIFFHWIMAALIIGLLGVGLYMVRIPISIEK
                     LKLYGWHKEYGFLVLFLAFFRLIWRLTNQLPELAIPLLEKIAARSMHWAFYFFMFAMP
                     ISGWLITSAAGLPASFFGLFVLPDLVAPDENRRILFQLIHEWLGYALIMAIILHAAAA
                     LKHHFINKDDILRRML"
     gene            complement(13363..13956)
                     /locus_tag="AC06IO_000663"
     CDS             complement(13363..13956)
                     /locus_tag="AC06IO_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIO9873876.1"
                     /translation="MKRIYRHFIASILAVFALPSYVYAEPQTLTLDNQHTYVLWKVKH
                     LGFSTQAGKWYASGQLVLDKDNPQQSKVNVTINVDDIVTGIPELDKHLKSKLFFDTKQ
                     FPTATFVSNRVEVMGKNKAKVYGMLTLHGVTKPIILNVILNKAGINLLNDRETAGFSA
                     TTSLKRSDYGIKALIPEVGDDVEIEIEAEAYLDKKYG"
     gene            complement(13984..14241)
                     /locus_tag="AC06IO_000664"
     CDS             complement(13984..14241)
                     /locus_tag="AC06IO_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HIO9873877.1"
                     /translation="MSMKDKLIQSTISAFLVLIASNTAIAEDLSKEKCYGVVKKGMND
                     CSTATSDCAGSSTRDSQKDAFILLPKGICEKLVGGSLDMGK"
     gene            complement(14363..15259)
                     /gene="iolE"
                     /locus_tag="AC06IO_000665"
     CDS             complement(14363..15259)
                     /gene="iolE"
                     /locus_tag="AC06IO_000665"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HIO9873878.1"
                     /translation="MEVKMSIQLGIAPINWCNDDDPNLGKDISFEQCINEMAMAGYSG
                     TELGNKYPRDVRTLKTALEQRGLQLSSSWFSTYFTEANAFEKTVSRFLEHLSFMRALG
                     ASYINVCECGHAIQGTDLPILSAHMPQFTQEQWSLLIQGLHTLGRIAYDFNMKIVYHY
                     HAGTGVFWEKDIDFLMTNTSPKLVSLLLDTGHAVFSGIDPVNLIDKYGDRILYVHLKD
                     IRPEVLRRVEMERLSFMDAVRRGVFTVPGDGAIDFIPIWAGLQKHRYNGWMIVEAEQD
                     PEKAHPLEYAKKAYDYLHSFIH"
     gene            complement(15262..17133)
                     /gene="iolD"
                     /locus_tag="AC06IO_000666"
     CDS             complement(15262..17133)
                     /gene="iolD"
                     /locus_tag="AC06IO_000666"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HIO9873879.1"
                     /translation="MKIKLTMAQALLRFLANQYVVLDNNEYHFINGVFGIFGHGNVTG
                     LGEALEYDAHGLIYYQGHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAA
                     TATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
                     LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAML
                     AEAIRHIRKAKQPLIIAGGGVHYSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMN
                     VGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAM
                     KMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQA
                     GLHQTTVLGLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVA
                     AGLGVRIAKENQEGEVYVLVGDGSFVMMHSELLTAIQERKKITILIFDNHGFQCIRNL
                     QEGNGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQFRTTLDS
                     AKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMREQIETVRQY"
     gene            complement(17172..19058)
                     /gene="iolC"
                     /locus_tag="AC06IO_000667"
     CDS             complement(17172..19058)
                     /gene="iolC"
                     /locus_tag="AC06IO_000667"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HIO9873880.1"
                     /translation="MKQFPGFYFDRNRAIDLICMGRVAVDLYAEQIGLDLKDVASFKK
                     YLGGCAGNIAVGAARLGLKSQMFSCVGSDELGKFLKEELEREGVNIELLSETDNHLTG
                     LVLLGIKPPSNFPLLFYRNDCADMQIKSDQISFTHLKNAKALLITGTGLSTESMKNTT
                     LEVVHLARKAETTIVFDLDYRPVLWRLTVIGNGESRYCQNEYVTQVYQQVLPLCDLIV
                     GTEEEICIAGGSNDIEVSLINIRQRTDVPIVVKLGEKGARVHFGADKGLLQAKSFPVE
                     VLNVLGAGDGFMSGLLAGLLQGKSWEQAVTYANACGALVVTRHGCAPAIPYKEELNYF
                     IQEYDHDPEIWSSSQLTQLHEKLSKNQKTQLLIKNPCGFADGLNSIVTMQDSPTAMSF
                     SSLKLNKGQSHQFNSSYEFAALLMTGKVVFQYGSYSQEVERTDYFSQSPYVLHCSKNE
                     VSSVIALTDCEILLMETENENLFFPILFDASNMLELDNRGEGVLENTSYRLVRTVFDK
                     RNRPESNLVVGEIITFQGRWSSYPSHYHQQPEIYHYRFSEPQGYAFGENGKEVMRIEH
                     YDTYQIANNQEHAHCTAPGYALYTLWFIRHLNNNAYTLPTFAKDHEWTRTSRANLRAW
                     QQKQ"
     gene            complement(19066..20070)
                     /gene="iolG"
                     /locus_tag="AC06IO_000668"
     CDS             complement(19066..20070)
                     /gene="iolG"
                     /locus_tag="AC06IO_000668"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HIO9873881.1"
                     /translation="MKKKICRIGIVGAGRIGRLHAENILFHLPQFELVAIADPQLDRN
                     WAEKLGITIVSTQSEDVLCHPDLDAILIASPSHLHLEQIITASIHEKAIFCEKPIGLN
                     EEEIEKTLNIIKNNNTLLQLGFNRRFDPGFANIQKRVQFGEIGTPHILRITSRDPACP
                     SKEYCATSGGIFMDMSIHDFDMARFLMQSEVVEVYATGAVLINPDFEQFDDVDTAIIQ
                     LRFANGAFGVIDNSRQAIYGYDQRIEVFGSGGMLLAENQLEHTVSRYSANGTDGAKPQ
                     YFFLERYHQAYLAELNAFYSAWNDNQTSPVSGLDGLQALRIAKAAKQSLETHLPVVLS
                     "
     gene            complement(20073..21488)
                     /locus_tag="AC06IO_000669"
     CDS             complement(20073..21488)
                     /locus_tag="AC06IO_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HIO9873882.1"
                     /translation="MNKGMNAFVFLIAVIAGFGGFLFGFDSSVIADVKDQVISQLALT
                     DWQWAQVVSSSLLGCILGIPISGLVADKLSRRSLLKVVALGFMLGSSLCALAPGFVSI
                     LLGRFIIGICIGIASYIAPLFIAEIAPPHQRGTLVLINGLTITFGQAVAYLIGYFLHD
                     YSLMSWRYLFWIGSLPALVLFSGMYFVPHSPRWIMMKYGADATLKTLKQIRPVGYNIQ
                     QELTEISNHLVNPAPAYIQMFKKPIVFVLLLGIGLGLFQQFSGINALMYYGPVVFESF
                     GFSPVKNAILATFFLGLVNFIFTVVTLFYVDKLGRRFLLISGTLIASISLYFVSVLSC
                     NPMFVNKFWILGGLSFYVMGYCISLGSLFWVLISEIYPLSVRGLAMSIATVFQWGANF
                     VVSLLFLPIYQFSGQTVTFAMFATFCLLACWFIYYFVPETTSVSLEKLEENLSSGKKV
                     RDIGQPVSKNIKIAKFNLIMD"
     gene            complement(21750..23423)
                     /locus_tag="AC06IO_000670"
     CDS             complement(21750..23423)
                     /locus_tag="AC06IO_000670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIO9873883.1"
                     /translation="MNTRIVSLLVVFMASANLQAASESIIWQAGDNNLKKYNVSILQD
                     KKGFMSITNKITPLNYQLKPVDHEGNRDYNDIRYQVYYRNIPVWGHELILHKTSKSKI
                     FLTGVDVSEIEKDVHQTDGKLSAADIESQIIKYNNNSIVYKNIKKIIYLDSHKKAYLA
                     YHLSMYANDPLHFVSAPNYIIDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLF
                     QHGDSMKDIPSLGKFEVTIKGKNCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNI
                     PAFFYPCSKESQYINLNDGDTSPANYSFSSINDAMYFAGVTLEMYKEYYGVEKPLGDD
                     LPLRAYTHIKNFDNAFAVPSIKIKGLYLMHQQIVIGDGDTQLTAPAQSTLSHELSHNF
                     TRLHSNLVYNGQSGGINEAFSDMASIAMQDYLRKDFPWYWDGEDWSIGREATISGEPI
                     RYMDYPTKDGHSIEHFDDYDDDLDVHLTSGIFNRAFYLLAHQPNWSVRKAFQVMVDAN
                     IKYWTSGTSFEAASCGVIQAAIDRQYNKQDVINAFKEVGVICPVIALVN"
     gene            complement(23465..24388)
                     /locus_tag="AC06IO_000671"
     CDS             complement(23465..24388)
                     /locus_tag="AC06IO_000671"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIO9873884.1"
                     /translation="MTSNKYDIVLLTQKDFIDPADITPYIQNVLTEDRLLTEALEKKG
                     LKVKRTHWDNREYNWSETRYALFRATWDYFHRYDEFHLWLNDCSKRIQFINPISIIQW
                     NIDKHYLSDMQQKGINIPPTLFLKRGRLNILNNLMKESGWKKAILKPAIAGGARHTYL
                     FDQDSADHYQDVFELLINNESMLLQEFQENIVNKGEVSFMVFGGRYSHSILKKAKSGD
                     FRVQDDFGGTVHPYEASQEEREFVENVIAQCDELPVYARVDVMWDNNNKLCLSELEMI
                     EPELWFRESSQAASAMADAVCEHISGVSEYK"
     gene            24570..25337
                     /locus_tag="AC06IO_000672"
     CDS             24570..25337
                     /locus_tag="AC06IO_000672"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIO9873885.1"
                     /translation="MFKNAIVRTPSSTMINGLTTSADLGKPNYELALIQHQNYIKALS
                     TCGLEVTVLPDLEQFPDACFVEDVSLLTEQFAVLTCPGAQSRRDEVQEIEPSIQAFFK
                     DRIFRINPPGRLEAGDVLRIDNHFFIGLSERTNKEGAEQLIYLLNQHGYTASVIQLKK
                     FLHLKTGVSYLNNDYVLVSGELINHQAFGHLKQIVVSPEEAYAANCIMINETVLLPKG
                     CPKITHRLSELGFSIIELDMSEFKKLDGGLSCLSLRF"
     gene            25337..26653
                     /locus_tag="AC06IO_000673"
     CDS             25337..26653
                     /locus_tag="AC06IO_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIO9873886.1"
                     /translation="MKSRLSVLSLALITVCSVDSIRNLPAAALAGNQLFYYFALALLF
                     FLLPCAIVAVWFSRQSEQGVYGWVKQGLGNQWAFIAIWFQCIQNVLIYPTLLSFIAGT
                     LLYSISPDLVQNKSMLFLIIIFLIWSLTWINLKGIHLSSRFNSYCSITGLILPFIIIL
                     FVGLYWWITQVKPGTTVLPPAESYSWTSLTAIILSFCGIELAAVHARDSKQGAFPKAI
                     AISVITIFLTMLFGSIVLAMIIPPEQLNFISSIPQLIQLFFNKIGYGYIAFIINGLIA
                     IGCIGAANNWLIAPIKGLEFAINEGFLNRQLSQVNKHSAPFRLLVAQAFCVSIISALF
                     LIVPSINASYWIMLNAATQINLLMYLLLFISAIKIVSSKPILSKLNIMIFAYLGLAGS
                     SITLIVSLTPPPSLAISSKLIYALLGAIFLVIMTLIPIWRKPKVLT"
     gene            26789..27826
                     /locus_tag="AC06IO_000674"
     CDS             26789..27826
                     /locus_tag="AC06IO_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HIO9873887.1"
                     /translation="MKLTRNMIAQAVQAKIITEEQAKNLIEFIKNLPEQTPGFNLTNV
                     LYYFGGLIAIGAMTLFMNLGWELFGGWGIFYLSVCYALLGLGLCSQFNGKGYVIPAGI
                     CATFVICLTPLAIYGLQKGMGWWPDDSVYHDYHVYIKWNWIFMELGTLMVGIILAWIY
                     RYPFMIMPIAVTLWYMSMDIAAMLGDGQFDFELRAMVSMYFGLITVLIAFWVDVRSSQ
                     SADYAFWLYIFGVMAFWFGLSCQSSDSEISKFIYMCINLFMILTGVILNRKVFVIFGA
                     LGCSYYLGHLAFQVFKFSMLFPVALTAIGFGIIYLGIIWQRNEAKLTKELRSILPKPL
                     RDLLEARDSMD"
     gene            27964..29445
                     /gene="legL3"
                     /locus_tag="AC06IO_000675"
     CDS             27964..29445
                     /gene="legL3"
                     /locus_tag="AC06IO_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL3"
                     /protein_id="HIO9873888.1"
                     /translation="MLTLSLAKCINATRNGAFAIKDLDLSGPLSYEDVLFLTEYLKVT
                     PITELDLSMTITRENSKALWDLSQVIAKNSLLQKLIFEVQLDFDFYFKHNLPIGWLDF
                     IDDYRLKKIAAPLHEALVNMLKNKPALNKLTIDFLAIETPFHNKYLNQLARYINKIPL
                     NYLNLGLSVTKETQVPLFKTKEPNQTLEFLILRGFNSGRNILKYLPQTAALKMLALDD
                     MQFEEHDLKLLANIMKSNSSLVALILGKTNLGQVNSPEVLTWLSACPNLNSLILIDNN
                     LSRLNVDSLCELLANNLQALKKLDLSKNAYMGDDAIKIAKALTFNTHIETLVLDDNYI
                     ENEGLQAIIDLVKTNKNITSLSMSNTCRYTPSNEIIHSLCDLLRDPGCQLKELKFNQD
                     ITLAQYLMLQHALNSNKTLEYIDFNFRNQNILEAILGKSNSGNLNENEFSSDNPKEEK
                     APNTLNTDPTSKSAPLFFISPKSRQEENKNELSSLPSHRVHLH"
     gene            complement(29539..30657)
                     /locus_tag="AC06IO_000676"
     CDS             complement(29539..30657)
                     /locus_tag="AC06IO_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1661 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873889.1"
                     /translation="MNTVPLKPQRLLSLDVFRGMTIVLMIFVNGQAAIDPYPIFEHVD
                     WNGCTLADLVFPFFLFIVGLTSVISLKNQMERKEKTSLYSAIIERSVVLFLLGLFLNV
                     FPHPIEFDSIRIYGILQRIAVCYLISAFIYLNTSIKTQFFIFLVLLLGYWIIMTQVPV
                     PGYGANQLTKDGSWVSYFDQLFFSASHLYEKTYDPEGFLSTFTSIATTLSGVLAGSLL
                     INPCNQFKKFYLLAGVGLLFLLLGWLWNMSFPINKNLWTSSYVLWTSGLALLAFAFCY
                     LLIDRLGVKKWSVFFKIFGMNALFAFVFHVLLLKLQYVFKITTPDGSKMALISYLKDY
                     FFGGFSNHNAALLYSISFLFLNFLVVLILYKRNVFIRI"
     gene            30818..31414
                     /locus_tag="AC06IO_000677"
     CDS             30818..31414
                     /locus_tag="AC06IO_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9873890.1"
                     /translation="MFSKKERTLLFANNLWYLSEGMLGPLFGIFCERFNSSPLDISWV
                     WSLYLISVGIVVIGLGYISDFIHKEKLLVAGYALQTIFTFCYIFITSKKQLALLQIGL
                     GISGGLSTTTWYALYARYEDKTHEGLTWGLANGLSSIYTGIALLCGGLIIAKTSFDVL
                     FIIMGCIQFLATIYVSKLIFIEHPKIIRRFRNPNLAKN"
     gene            31579..32091
                     /locus_tag="AC06IO_000678"
     CDS             31579..32091
                     /locus_tag="AC06IO_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873891.1"
                     /translation="MFESFRKCLSAISMRAPYASTPGHRNAQRANAGSEVLGTTLETF
                     DPAASIPTKLAQTLISIYSLFRFDTHVSEKLIHLLQGSIAATQMGLGIALLFTGTECE
                     EYTDADLCKAIFLLQLLYRGTLLVGWAPSEFSKDPYAEPKVVSEDKAESDAESEEENE
                     DEESRNSASV"
     gene            complement(32150..34456)
                     /gene="pbpC"
                     /locus_tag="AC06IO_000679"
     CDS             complement(32150..34456)
                     /gene="pbpC"
                     /locus_tag="AC06IO_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HIO9873892.1"
                     /translation="MRKVIKYFSIILGSLFVSGFLLLFFSPKPSLLEEFNFSKAVYDD
                     NQQLLRLILSEDDKYRLYVPLSQIPKELMEATLLQEDQYFRWHKGVNPFSMMKAIWQT
                     YVVKSRRIGASTITMQVARIRFGINSKKISGKLWQIIRALQIERHYDKDKILEAYFNL
                     APYGGNIEGVGAASLIYFNKPVSKLGLPEALTLSIIPQNPVKRTSKNSELKKIRNRLF
                     ARWLKQHPQDQEKQPLFGLPLVMQTNQNLPFLAPHFVNMVLKENPDLSQSISTTLDYR
                     LQLIVERITRHYLARKKMFGVHNAAVLLVDSRTMSTKALMGSADFFNNKISGQINGVE
                     TKRSPGSTLKPFIYGLALDQGLIHPNTVLKDVPQSFNGYNPENFDYDFMGPIKAKDAL
                     VLSRNIPAIELSNQLTNPTLHKLLEQAHVTQLRSESYYGLSLNLGGVELTMKELVGLY
                     AMLINDGFWYPINSLKGVPRQKGRRLLSTEASFLVFDMLKNTPMKESNNDGSLQLPIA
                     WKTGTSSGYRDAWTVGIFGPYVLAVWIGNFDNQANPAFIGKEIAAPLFFELIDAIKHE
                     RGPIARVEKYPEHMHLEKIEVCKASGMLPGRYCTDTEWTWFIPGKSPIKTDTIFREVA
                     INGKTGLRTCHIDENTRFEVYEFWPSDLLRIFRRAGIQRHTPPFFEPGCVLSGNSGIS
                     PQITSPQAGVSYIVHANTQSNKIIPLTAVTDAGIAHLYWFINESFVAETKSDEPYLWQ
                     AKPGKYVVRVVDDRGLSDARGITIQMVS"
     gene            34634..40399
                     /locus_tag="AC06IO_000680"
     CDS             34634..40399
                     /locus_tag="AC06IO_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HIO9873893.1"
                     /translation="MNKNPFSLIMNVLCSIFSTLFGKIKWNSPTWLVYLKHKSKNSPQ
                     IFWGTTLLFIVLLGAGACGAYWYNNLPKPTYTTASITIPDITPNEENLVPNNLIIDFG
                     IRNNEFIPQSVAPINLIGKEIDEGIEMAPQIAGKWSWNSDSQLIFIPAEDWPAGQKYT
                     IHFAKNFFAPNANIESYDLSFTTKPFQATIREFKFYQDPVNAETRHAVATIEFNYPVN
                     PKNLEKNTSVVFQTIKNGKADLTAESVPFSYTYDNNKRIAYLHSANIKIEDVSRYLLL
                     TLNKNVSSSTDSAILGQPISKNLLIPDASNFLKIVSTSANIVRNDKDRPEQVLSIETS
                     LGVNESEFNKSVHVYLLPQNYPATAAEPEKSNYEWQNPGEVTNNILSLSTPLPKEVIP
                     SEQNYTTLHGFKFKAQTPRYLYIKIDKGMKGFGGFKLSNDYTAIVPVPEYPKEISFLH
                     KGSLLALSGEKKLSVLIRGVPAVKYEFARVLPDNVNQLVTQTQGDFSNPYFINPSFNQ
                     QNISQIFSEIQQYDSTDLTRQQYTSLDLGKYLASEANVSGPQGLFLLQATGWDVANQY
                     ALDVKASRLILITDLGMMVKDNNDGSHDIFVSSITQGLPVAGATVTILGKNGLPILSR
                     VTDEQGRANFPTLKDFIEDREPTVYLANLNNDVSFIPFNNYNRQLNFSKFDIGGIYTN
                     DQDLQNLTAYLFSDRGIYRPGDTVHVGMIVKRAFAEPQPSGLPLQATVVDARGTTIKD
                     QKVTLNDTGYMELDFTTSTNSPTGQYMVYLYLVKDNHPQNLLGSTSVRISEFQPDRMR
                     ITSSFEPKPFDGWSSPTDLKAKVDLWNLYGAPAANRRVSGKILLTPQRVQFDNYPDYI
                     FADPLYDPKKPPKVFTENLAEATTNENGQAEFNLNLERFEKATYQLTFFAEGFEPGSG
                     RSVTTQAKTLVSPLPYFIGYKADGDLHFIKQNSVRNVNYIAINPQLGKEDVKNLKIQL
                     ISLRPVTTLVKKADGTYQYQSTIQSTVINTNPFEIKESGVNYTLPTNTIGDYSLNVLD
                     KDDSILSQLQFSVVGTSQAPLAKNAELSIKLNKEEYQANEDIEIQITAPYTGSGLITI
                     ERDKVYAVHWFKTEAQNSVQTIRIPADFQGNGYVNVAFIRDWESPELFISPLSYSVAP
                     FNVNHDKHDIKIGLKTPELARPGEAFTIDYHTDKPGKIIVFAVDEGILQVANYTTPDP
                     LSFFFQKRALEVLTQQTVDQILPKFIQDREISAVGGDEGEAALASRLNPFKRKTDLPV
                     VYWSGILDADSTDRQLSYQIPDYFNGSIRVIAVAVANDSVGSREVSAEVRGDFIISPN
                     VPTFVAPGDEFEISATVANNIKDSGEHAQIEVELSASPELEVIGSAKESLEISEGNEQ
                     TVYFKLRAKSLLGSARIAITAKSGDKSSKMDSTLSVRPANPFTTYINSGKSQDSQKSL
                     EVIQTLYPEHRKVEATISTSPLVLIFGLQRYLDNFPYGCTEQLTSKALPWLVMNSLPW
                     FSSESGPIHEKINTTIQMLSQRQMSSGGFSYWPGLSDNSANSFASVYAMHFLTEARAQ
                     GFNVPGEMFYNGINYLKELASRNPTDADSARVQAYAIYILTRNELVTTNYLANLQLYL
                     QQDSSKNWLKDITAVYIAATYQLLKSYSEANQLIGQYQPATNNTFTTDFYDRNIADAQ
                     YLYLVAKHFPNLLPQVSDNILMNLVKAINNDEINTVLSGFSSLALGAYPQNESAEQSD
                     KLSITEVLANNQQRNIPTDQNNYQKLNIDETVKTIRFNNPDKQTFFYQLVQSGFDKII
                     PTEPLKQGIEIYREYRDEKGNVIDSAVLGEEIEVHLQVRSLDNNYLSNIAIEDLLPGG
                     FEVVRDSVKTDNMDYADVREDRVNFFGWVDLTAKELVYKIKATNVGKYIVPPAYAEAM
                     YNPNTKARGSSSVITVSREED"
     gene            complement(40544..41947)
                     /locus_tag="AC06IO_000681"
     CDS             complement(40544..41947)
                     /locus_tag="AC06IO_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1666 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873894.1"
                     /translation="MLKFVIYLSSRVQLMGKKAYIIGMGPAGLASALALLAKGYSVEL
                     IDRRSEDDVFSRKQGVFLKDDTIRDFFALSQLASKGYSLEFKDNFICKLIRPIDESNL
                     QLSAEDQLDLAFFELIEKERTVIPIAELQRYQYSKLKYIYDKGGFTFQDEGDPYTVKF
                     NNAEQQLIFHLGDTQIAKVDAENQELTLDHNGSIQNHSFDLLVDASGKTSKSFTQLWN
                     SQNPEFAIGYEKLDNPDHNAFGVMYLSINNPPLAMIANPVLSPTEGTSFISLDKITPL
                     KAMGWTHDYPPLIYLKYSKKNGAVYLTGEIPESILKDNNKKELKQWFDLVLQLLFNNT
                     NFKTSAPGLASVFKTDIEIADKFVLLLPKGGGFCLVGDALMSANFLLGCGISNALDDA
                     NKLPDMVDNQFSQSEAEAYRNLRYLDYKDDWLFFREHIALRLKSLQLEKQLDLSRQEL
                     ELKQRELENIQQQCSFY"
     gene            complement(42108..43499)
                     /locus_tag="AC06IO_000682"
     CDS             complement(42108..43499)
                     /locus_tag="AC06IO_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1667 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873895.1"
                     /translation="MQYKEVTPMIRFFIICFIFLVSHLAYANPYWDRYKVPRDSHYPN
                     LKFHYINPDSLKIVQENAGIDVLRIQTDWFEETDSYQVGTVIIESSGTPALLSRSIHK
                     PKWGSYLGILKSRFGGDTVYYDAIGTGKEYRKLARAINLRFPVPSHDMIFELYAENPQ
                     TGLMEKVVEKNIIASELTRDNRYFNDLEVRQLLPAVKTPSLRVNIYAEGYTQSEKMKF
                     WNRAIKTVQALQSQQFPGIDYMDFYAVFNPSHKRLGVAEDLGLPVPEFDSFLGLYYPY
                     WDNFGRWYHIVYPTRENKFRQGLASAPYDYPIVLVNNSQYWGVGNYKSHTAIPADNST
                     YFTYILIHELGHFFGLNEEYEGGGRTELEFASEMKEPWSQNITFLTTPSHSGLKWNEL
                     VNPQMSIPTPYSDWQSTPPQYGAYRGGYGDSPSHQGASHKPGLNCVMESHASFCDICQ
                     KGIMDVIRFDLGT"
     gene            complement(43647..44471)
                     /locus_tag="AC06IO_000683"
     CDS             complement(43647..44471)
                     /locus_tag="AC06IO_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HIO9873896.1"
                     /translation="MKKIAIILSLFPFLICCGLASATASQKRLPGESYQDAVKRIINN
                     KPQNELKMLIARGTPYSKKVSLQKIDYSTVPKVSSYEELANMFNLIRDTRFLYSPDKP
                     DFQRRISWLYPDDGCFARAALSRIKLDSEHFVIPAKIFVFGDLEMQTPYSSEGTVSWW
                     YHVSAVVNYMGTIYVLDPAAKPEGPMLIDDWYNKIGIMDKMEAVVCNPYTYDPFDRCY
                     DATNKTDNKALNDQLKYLDKEWVRIESLGFDPSVLLGDNPPWIGDMGNSTLLSYKS"
BASE COUNT        14196 a         8997 c         7791 g        14046 t
ORIGIN      
        1 gctaatttaa aaaaatgaga tatatcattt tcacttttag ggaaaatgat ggcttcagga
       61 ataatttgat aaacactatt atccgttgat aaaacaattc ggctacttaa agcttcatca
      121 atctcaccgt gaaaatcggg tattttctca agttgctgta aaaataacct gatttttctg
      181 ggtgttgcag tggaagaggt caaacgcggg atcataattt tttcctagct tttcctccat
      241 taatcttctc attatagact accagataat taggactaaa tcataaagac ggcaatcatt
      301 caatgagaaa aaaagtcttt ttaggctaaa ttgaaggaat agagctattt tttaaaggag
      361 taagacaaga tgaacaaaat aacagaagat tctctgactg aagaacaaaa atacatggtt
      421 caagtatggg aaaatcatac ctattatgaa tttgacacta aaaatgttgc agatactatg
      481 aaaaccatgg cagacaatcc ctatgtcaat aacataccta ccatgacagg cggatttgga
      541 aaacaagaag tcaaacaatt ttacagtcaa ttgtttatac cccaaaaacc agatgatact
      601 gaggttgaac tgatttcaag aacagtaggc gctcatcaaa ttgtagatga gctcattttt
      661 aaatttaccc ataatatcag gatggattgg atattaaatg gcatcgagcc tactggaaaa
      721 cgtgtagaaa ttcctctaat tgcgattgtc agatttcata atagaaaaat cataagcgaa
      781 catatttatt gggatcaggc atccgttctt gtacaactcg gtttactcaa ttcagataaa
      841 ctacccgttc atggagtaga agccaccaat aagataaggg aactgatatc acgaagtctc
      901 actaagtaag aaaaaacgct tacttataac tataattatt tttaaagaac agggatcgtt
      961 atgataaaag attatcttat agaaaccatg aatcaggatg aggtgaatct tgctataacg
     1021 tgggctgccc gggaaggatg gaatccagga attcatgatg caacatgttt ttatcaaact
     1081 gatcctcatg gtttttttgc aggtaagctg aatggaaaaa cgatagccat tggttctgct
     1141 gttatctatg atgaattttt tgctttttgt ggcttctata ttgttgataa accctatagg
     1201 gagcaaggtt ttggtttgga attaactaaa gccagattag catatattgg taacagaaat
     1261 gcaggaattg atggtgtgat caacatgctg gataaatatg cccgcattgg ttatcaatta
     1321 gcacataata acgccagata ttgtcttaat aagacaataa aaccaatatc atctgaacct
     1381 catattgtac cattaaatca aatcgatttt tcgcagttat catcctatga ccgcttacat
     1441 tttccagcac ctcgtcctca atttttaaaa tgctggataa gtcagccaca aagcttggct
     1501 cttgcttatt taattgatgg ccaaattaaa ggttatggtg ttatcagaac ctgtcataaa
     1561 ggatttaaga ttggaccact atttgcggat actccggaaa ttgcacacca acttttcact
     1621 aatttggtcc catatgctaa gggtcaattg atatttctgg atattccaga gaataaccct
     1681 ttagcaattg agctggtcag tcaacatcaa atggagaagg tattcgctac agcacgtatg
     1741 tatttaaaag gagaacccct ggttaaaaaa gaaagtattt atggaattac tacttttgaa
     1801 ttaggataaa ccatgacccg agatcttact ggatatggat cagaacaagt taatttgacc
     1861 tggcccaata aggctaaaat cgctattaat tttgttatta attacgaaga aggggcagag
     1921 ttaactccac ttaacggtga tacaaaggct gaattttacg gaagtgattt tcctttcgtt
     1981 cctaaaggaa atgcagaaag gaattatagt atcgaatcta tttatgaata cggaagtcga
     2041 gttgggattt ggcgtttaat ccgtcttttt gacaagcatt ccatccccat aacttttttc
     2101 ataacaggtt atgccttaat attaaatcct gaattctcag gctatcttgc aagtcataac
     2161 catgaagttg ctgggcatgg atggcgatgg attgattatt caaaagaaag taaggcaacg
     2221 gaaaaaaaac acatctctcg ctgtattgat accttgcagg aactcactgg acaaaggcca
     2281 ttgggctggt acactggaag aagaagccca tttacccgcg atctgctaac tcaaattggt
     2341 gggtttgtct atgactcaga tagttacgcc gatgaactcc cctatttcat taacaagcat
     2401 cttgtaatcc catactcatt agactgtaat gattttcgtt tcacaatttc tcccggcttt
     2461 gtcaccgcac aggatttcta tcaacgtctt aaaaatactt ttgattacct ttatcaagaa
     2521 aatcgattat ctatcatgac tattggttta catccgcgat taagcggcaa acccgatcgt
     2581 tgccatgccc ttaatgaatt tctaagctat atccgacaat ttcaaaatat atggatagca
     2641 aagagaatag atattgcact gtactggtta accaaacaca cccagattaa gcaggattta
     2701 tgaaataccc aatctcatta ttaagaaatg tttttaattc agtcgtttag ttcatctaag
     2761 attcttcgtt aaaacatttt ttgccttgaa cgtgaggttt ttcgtaatgc tcttatagcc
     2821 tgatcttatt aatttctgta ctgtttcatc aaaagagctt aaaattcaca tctcaaaatc
     2881 tttataattc accccactat aatttacaga ttgccaatat gagtcatttg ctagatttat
     2941 ccatccttaa aaaaaatcgc gactttacct tgctttattt gggccaattc atttcattca
     3001 taggcacaat gattaccagt gtcgctcttc cctatcaaat ttatcatttg accgaatcga
     3061 ctatgatggt aggattactc agcctggcac aattactacc tctactgatt actgcattgc
     3121 ttggtggagt gtttgctgat cgatatagcc gacgcggatt actaatcatt agtgaattat
     3181 tacttgctgt tggttgtctt tttctggctt tcaattccaa actggttaat ccaagtgtta
     3241 gcttgatatt tataatagcc tcattaatgt ccgccattac aggtttacac aggcctgctt
     3301 ttgatagtgt gatacaacaa atcgtgaaac cagaagatta caagaaagta ggcgctcttg
     3361 gcagcttcaa attcagtttt tgtatgatta ttggaccagc tgctgctggc ctgattattg
     3421 cgcaatatgg tattgtaatc acttatctca ttgatttatt aacctttatc atttcacttc
     3481 tcaatttgag cttaatgcac accataccca agcccttagc caaagagcat ccctcaatta
     3541 tagtttcttt gaaagagggt atagattttg ctttgggccg acaagaactg atgggtagtt
     3601 atttcgttga tttctttgcg atggtttttg ctatgccaaa tgcattgttg cccgctattg
     3661 cccaacaatt tggaggagct aaaacactag gactgcttta tgctgcgcca gctatagggt
     3721 cgctgattat ttcctttgtt agtggctgga ctgcaaaaat tactcatgat ggaaaagcca
     3781 ttgccattgc tgccgccttg tggggagcag ccattatcgg atttggactc accacttctt
     3841 tgtggttatc tctcttgttt cttgctcttg caggagcatt tgatgccatt agtggtattt
     3901 ttagaagcag cttatggaat cataccatcc cgcaagaatt gagaggccgc ctgtctggaa
     3961 ttgaaatgat tagttatttg agcggcccaa gattgggtga tacccgagca ggctttgttg
     4021 cagcaggttt aggcatacct gctgcattaa tttctggcgg tattttatgt attataggag
     4081 tgactgtatg ttgctatgcc ttgcctaaat tttggaatta tcattcaaag taattaaccc
     4141 ctttactaat ctccaataat agaggctttt ttatgttgtt gcttggtagt ctcatccagg
     4201 ctggcagcct cagttaatgc tgccacattt tctttggtaa aaaatccatc aaaattgtgg
     4261 catctctcaa cgtattctgt aattaatatg gaatacatag agtgcttgga gaaaatttgc
     4321 tttaaatcgg gtgagtcctc gcaagcccca aatactttag ctaaaaattc aatatttaat
     4381 ttatctcgaa gagtttcaac gacaaaatca atattctttt taccagaaga gtgatccccg
     4441 tcttttactt cataagcaat atgatgcatt cttcttccgt agttacgcac aaaggtttct
     4501 gtgggcatag ggagattttc aaaggaattc accatgaacg gagtgttgtt agcagtaaac
     4561 acttttgccg gcgatttgat gtcatgtccg tgtggatttc tatttacatt ggtagaggag
     4621 ttcatatcat agatattata agcaccccaa aaataatatt tggataaaca taaaaactct
     4681 aaaatagcat cctcacgttc accagccagg attcgagtag ccatatggtc aatacctttg
     4741 atcaagtcct tgatcccatt atcgtggctt tcctgatctt tcgcatccag catcgcttct
     4801 tgttctttgg ttaaataaaa tcgttgtccc aattccagac agtcaaaatc ataaatatta
     4861 tcatcggtat acccagcgca attgtaggtg aaatccgata accgggtaaa atgtatatgt
     4921 ttattacaat agaacttgtt ttcagtcata tcaggattat gaaaattaaa atcatgagaa
     4981 actaatatgt tacgcgtctc atccttatcc ttgcatttat aaatctcacc tacataacga
     5041 gcattgaatt tggctcttga gttgggatac atcttgttta atcttgtgat atcgtcttta
     5101 aaattaggat ccaggggctc aagcaccaga aaagcaggag aatttttggt attgagtaaa
     5161 caataaattt tatgtgtatc actaatataa cttcgagaat atcgataagg ggtcattgaa
     5221 tagagttctt taatatatgg aatagcatct cccgtttgta cctgaacaac taccccacac
     5281 atatcaccaa atagatcctc aagtccggat gataaacgcc tttcgtaaat cttggtttta
     5341 tattcattaa aatagtctga gttgacttta tcccctttgg gatggtattc aattggatta
     5401 atcatcatgt tctccccaat ttgattcacc ggcacataat attttaagta tatacctgcc
     5461 aatgaatttt tatttgaatt aataactgtt tcctctcttt catggcaaaa tagaggtata
     5521 ataattcatt ttgattcctt acatactttc caatggaagc tttaatcgta tcacttgggg
     5581 tgattaccct tgcagaaatt ggcgataaaa cacaattact cgccttcctt ctggcagcac
     5641 aatttaaaaa gccattgcca attattttgg gaatattaac tgctactttg ataaatcatt
     5701 ctttggctgg actcatcggg atctggatta ccactttatt aaagccgaat gttttgcgtt
     5761 ggatacttgg catatcgttt attctaatgg caatatggac gctgataccc gatgaaattg
     5821 aacaggatga gaaaaaaata tccaaatatc ttggtgtttt tggagcaacg tttatcactt
     5881 tctttctgtc agaaacagga gataaaacac aaattgcaac tattgccctg gcagcacact
     5941 ataactcccc aatcctggtt attgtcggtt caacccttgg aatgttgctt gctgatttac
     6001 cagctgttta ttttggaaat ttattttccc ataaaattcc catgaaaata attcatgctg
     6061 tagctgcatg cgctttttta attattggac tcattaccat cttgttttga ccgactaggc
     6121 aggattgtta tttaacataa gcatctagcc agttggtata tctttttaat ctgtcgatga
     6181 caaagcttgg tttctccaaa gtatgatatt cattagggta aataaccagc tgtgtgggca
     6241 catcgagtga tttcaaagct tggtataatt gttctgaccc aacacaagga acattaaaat
     6301 ccaagccact gcacaaaaat aatgtgggtg tttttatatt attcgcttta agaaaagggt
     6361 agcttaattt tagatacaat tcaggattgg tccagggctt tcctaattcc agctcgtatt
     6421 ccggggtata ttgatcgaca ccataaccag ctaaaatatt cgctgctcca gcacctgata
     6481 tagccgcttt gaaacggcta tctgtagcta tgacataatt ggttaacatt cctccgtaac
     6541 tccatccgcc tattgccaaa cgatttggat caaccattcc cttccctatg acataatcaa
     6601 cagaagccaa tatgtctttt acatccaaat taccccaatc tgcaaaaatt gcttttgcat
     6661 aatgaaaccc ccttccggaa ctccctcttg gattaggagc aatgacgaca taattattgg
     6721 ctgctatcca ctgcatatca aagttaaatt catggctaaa ttgatagact ggtcctccat
     6781 gcagatagac aaaaccaggc aataaaatac ccgactggta ttcagctggc ttcattaata
     6841 atccatcaac tcttgttcca tcaaagctat tgcactgtat gtcttctgcg ggttgaaatt
     6901 taacttcatc cagtagcttt ttattatgtt gtgttaaagg aattaatccg gaattcaaaa
     6961 taaataattc agagggatgt gtatcatctg tggttaacaa aataattttc cctttggcaa
     7021 gtgaataatc catgtcatag cgcaagccgc ctgtcaattt ttttatctgg gcggaatgaa
     7081 tgtctatgcg attcaaatag gtgtttctgc tttcttctat taatgcataa ataaatttgc
     7141 catcttgaga ccattttggc ttgtaatacc aattatcccg aggtgcaatg attttctcat
     7201 ctttagtatt gatatcaata attgccaatt gattgggcga ataataggtc cattgatgtt
     7261 cattactgcg aagatacgct atttttgaac tatccgggct ccaggaaaga tttgaatcat
     7321 aatcaggatt catatcacta ccggggaaat gagtgagttg gcgctctttt gccccagact
     7381 tgggttcgat caaataaata tcataattaa aatgcctatc cggatctttt ccacgctttg
     7441 tgacaaacgc gatgtatttt ccatcaggtg accaagccgg taaatattca tcatgatttc
     7501 catcagtcag ttgagtaata gccttactct taagttcaac caaatataaa tgatctcttc
     7561 ccttaccaag ataaccgaca ccatcaacct taaattgata tctatctaca actatgggtt
     7621 gttcttcctc attagatgga tcactcttct gagctataaa tgcaatcctg ttagaatctg
     7681 gggaccatgt aaaatcgctc acttcaccat caatataagt tagttgatgt tctttattat
     7741 cctggactga tgctatccaa atttgtgtcg taccctcttt tccgccatcg gataagtaag
     7801 ctaaccattt accattagga ctccatttgg gtagcgagct tgacgaatct tctgcaaagg
     7861 taatttgttg actggattta ctatccactg atactttcca tacctcagtc gtggaaatgt
     7921 tatctttggc atttaattgg gatacggtat aggcaaccca ttttccatct ggtgacagat
     7981 ctggctcgct gatatcttgt atacgataaa gatcatcaag ctctggcaaa cgcttcccag
     8041 cccatccatc atgaaataca acaagacaaa taattagacc caggcatacc cccttcatga
     8101 caatcatcct tattatgctg aaaaatcaga gatttatagt gccttaaaaa gtatagaata
     8161 aatttgttat atatgatttt tccggcaatc agcaaacaaa ttttgagtag aataagtaca
     8221 gtccggtgtc attggtttag aatcgtagct gaaattcttc agaaataaag aaatccccgt
     8281 ctgttcaacg aggatcataa aaaggttcta atttatttct ggtatacttt ttacggcatg
     8341 atccgcttct gttctttgaa caaactctct aaagaaagtc tgcgcgctct taccagatcg
     8401 attatgcaat gcattccagt ctgaattatg tccattagca tattgtacat cagtctccat
     8461 ttttctgtca cttttgattt tttccctagc ttgaactaaa gcgtctcttg cattagccaa
     8521 ccctggtttt gcaagaggat cagcttttcg aatgatatca tccagatttg caatttcttc
     8581 atcaatctcc gcctttttct tatgccgctc agaagacagt tctttatgga tagatgcata
     8641 atgtggaatc acagcatcat cgatacgctc gccccttatc tcttttcttg acctaaccac
     8701 tatgtaatct ttatatgctt caggaacact cttgaatgca ctgcctgcat taatttccca
     8761 tttagctaaa gcaacaccga gtttaatgaa gggcttagcc aaccatccaa gtattgtccc
     8821 tactgtgctg tctttatgac ctattgctcg accaatttca tcacgctcta aacgcatatg
     8881 ccctactaag aaattagtaa tagtagagaa agaacggcta ttaaatagat taattggctg
     8941 tccaagttga tgaaaatatt gcgctaccaa ggaagccacg cctgcaccaa gagaatgacc
     9001 tttaagggta atattttgtg gcgaaacacc ttgatctaat agtctttgca cttgtgcaat
     9061 accatctgca accaaatcct cacttgattt ggctttgcct gtactttgac caacaccacg
     9121 aagattaaaa ccaataacat tggccttaag agccttcgaa tcttctttta tatcatcaat
     9181 gatatgttca taacacatac cattccctac caaattgata atatattttt ggtatttggg
     9241 atcgatactt tcttgtgacc tatgccttac ttcaaatgta tctaaatgcg ctccatcatg
     9301 agtaattaca tcatgtttct cacaaatcag ttcgtcttca ttataattac ttacagtatc
     9361 atcactaaca gctaaatcat caaaattttc attttgtgct ggcagcacta aaccactaac
     9421 ccattcacca agcagtttat ttgccccaat ttttattaaa tcccataata aaacaggggg
     9481 aaaaaccgtt cgaattgcaa taaatttaaa ccattcccaa ccactatatt taggttttgg
     9541 tgcattataa attttaacca tgagtccaat cctgtttaca taaaattgtt catattatat
     9601 acagttaata ttaatttaac cttaagctga atattataaa cttaaaaagt tgttctaatc
     9661 tgggaagtaa aaataaataa aggatgcgtg atcgctcaat attctatcat tatgctgact
     9721 tagcttatga gtttgagcca atcaaagacg ctggtgatat ttaaccagtg ctattatatg
     9781 ccatcacaac aatttgcttc tgtaatggcc ttcattcaac taagaaatga tacaaagaat
     9841 gatagtttgg ctctatgatt gaataatgtt gttttctgcc taaaaggatc ttcagctgtt
     9901 ccgggacagg gatttctttt ttcaatagtg gttctaatac ctcttcaaat ttggccgggt
     9961 gccctggtgc cacaataacc cagggtttat cctgattgat tgtacttaga cgatgataag
    10021 ctgtggcggt atgagggcaa attatataat gatatttatt gtaacaatgt aggatagctt
    10081 gcttaatttg ttcatcacta acagattctg cattcatctg ttttttacat tcagaataat
    10141 tattaaataa aacgaataag cgctccaaat tgcttggctc acctacatcc atcgctgttg
    10201 ccaatgtttt agtaaccgat ttggcttgat aagctcccgt taacaaaaat tcgcccagtg
    10261 cactattagt attattagct attaaaattt gctcaaccgg aaaacccagc atttttgccc
    10321 aataacaagc cgtaacatta cccaggtttc cgcctggaac tataaaatta acagaataat
    10381 tatgtcttaa tgccagttga atgctggtaa aagcatagaa aataatctgt ggtaataatc
    10441 tgacaatatt catgctattg gctgtagtca atattccttt atgttctctt ttggcaaata
    10501 ccagcttcac cagctgttga cattgatcaa aactgccatt aacagccaat gcacgaatat
    10561 tctcccccca acaagtaatt tgagattgtt gtctgaaagt aagtttatct ttgggaaata
    10621 aaatgattcc ttccagatct cttttcccat gcaaagcatg agcaatcgca gcgccagtat
    10681 caccagatgt tgcgaccagg actttggcgc tcttgttttc tatcagatgc aataaacatt
    10741 cactgaaaaa ttgagttcct aaatctttga aagacagggt tgggccatga aacaactcga
    10801 gtacataact tttcttaaaa agatgatgta gcggaaagga gaaggtgagg aatttttgaa
    10861 aaaaagcatt atttacatca attttactat ctgaaaaaaa tggcgagagt aaatgagcag
    10921 aaaagtcgat taaattaccg tattcataaa atttttttat tgttaaatcg gggaacctgt
    10981 ccggtacaaa taacccgcca tcccatgcca ggcctgacag tattgcttcg tctattgtgg
    11041 agacacattg tcctctcgta ctctttattc taattttcat ctgcagcctt tgcaccctct
    11101 gatgaaatat tggtaaccaa taaatcactt tcaaagacaa cttttgcttc gcggtatatt
    11161 gctaacagct atcttttagt caacctaatt tagaagataa tggaagttta gcttattttt
    11221 tacaaattta ttaagattga cgtgaagtaa catcaggcaa aacgccaatt taaacctaat
    11281 ttgaattgtt ctaaaattgg atgcttggcg attctggtcg tttgatacct atgattatta
    11341 gaatccctac cctgtgaatc aaagataaaa tcctgcaacc gattccaatc ccactctaca
    11401 cggcaacgcc aatttttggt taactccaac tcgaggcctg tacctaattg gactccattc
    11461 ttttttttgg tatttgaaaa tcttccactg aatgagccaa aagggccact ctgagtaatg
    11521 cttccactta ggttgacatc actgacaatc cagcctaccc gcccatagat gattcccaca
    11581 gaaggcaaat aaataccagg gcgtatagct agaccagact gccatttttg tttaaatgaa
    11641 ccctttaata ttcttgagta aagttcgtta gaatcaaaaa aatagatttt tccttcattc
    11701 gaactgcggt taaaaaaacc ttctaaacca atataataat gtgtccggaa aaatttttca
    11761 tagcctaaat aaatttctcc tccaagtccc gaaccatgcc agtcaaaatg atcaaagtca
    11821 gtaaaattga gctcttggct ggtttcattt cttttaaaat tgtaatgggc tgtgtctcta
    11881 ataactgaaa atccaaaata cgtatcgggc cttagaaaaa aattgagcgg tgttttctca
    11941 gccgctaaaa caaataaagg tgtaaaactg aaaagtaaaa acaacaaaat tgattgaaca
    12001 ggaaaaccta aatacacgaa taattccttt taataagtca aaaaatagcc attatcttct
    12061 attgcgcgtc actattttgc atcaataata aaaataataa ttctacgtat cttgtgacca
    12121 aaactctata ttttcattag aaaattctgc caaacgcatg cataatacca ggtcaggtaa
    12181 tgctttaaaa tgtgaaataa gtttaattat ttcatatggt tgcgtattta tagattatat
    12241 attagccttg caattatttt tttcgtgttg cagttatttt aaagcgtacg atcacttcat
    12301 cttgtatttc atcagtacta gcccattcac cttgtcctac cccaaatgta ctgcgtttga
    12361 ttgttgagct accttcaact aaagcctgat cgttagaaat ttgctccaca acaaacttca
    12421 gagtaaccgg agctgattta tctcttatag ttagagtccc ttctgcctca taagttttat
    12481 catccaattg attaaatttc gttgctttaa actctgcgtt aggaaatgtc ttgacgttga
    12541 accagtcagg ggaagccaat gtcgttacga tgtcctcaaa aggtgctgca atcgaattca
    12601 tatcaataat gatatcaatt gaccctgtct tgtagtttga agggtcagca aaaatttgac
    12661 cagtaaattt cttaaatcgc cctgtaaccg gggcaccatt ttgagtacca gtaaaagtaa
    12721 tctcactttg tgctggcacg atttcccatt cagggagttt actggcgtaa atcaatgtat
    12781 tcacaaaaaa tatcaatacc caaagggaag atttaatata gacattcact ataacattct
    12841 ccgtaatata tcatccttat taataaaatg atgttttaat gccgccgctg catgcaggat
    12901 tatggccata attaatgcat accccaacca ttcatgaatc aattggaata aaattcttct
    12961 attctcatcg ggcgcaacaa ggtcgggtaa aacaaataaa ccaaaaaaag aagcaggcaa
    13021 tcctgcagcc gaggtaatta gccatccact gatgggcata gcaaacatga aaaaataaaa
    13081 agcccagtgc atgctgcgag ctgcaatttt ctctaacagg ggaatagcca attccggtaa
    13141 ttgattagtc aaacgccaaa tcaatctgaa aaaagccaaa aacaatacca gaaatccgta
    13201 ctccttatgc caaccatata attttaattt ctcaatacta atagggatcc tgaccatgta
    13261 taaacctact cccaatagcc caataattaa agcggccata atccaatgga aaaaaatcgc
    13321 aaccagacca aagctggttg aagtattttt taattgcata gattatccat atttcttatc
    13381 aagataggcc tctgcttcta tttctatttc aacatcatct cctacttctg gtattaaagc
    13441 ttttattcca taatcagaac gctttaatga agtggtagca ctaaacccag ctgtttctct
    13501 gtcatttaat aaattaattc ctgctttatt taatatcacg ttcaatatga tgggttttgt
    13561 gacgccatgc aaagtaagca ttccatatac ttttgcttta ttctttccca ttacttccac
    13621 tctgttacta acaaaagttg ctgttggaaa ctgcttggta tcaaaaaata atttactttt
    13681 caaatgctta tccaactcgg gtatacctgt gacgatatca tcgacattga tagtaacatt
    13741 cactttactc tgctgcgggt tatccttatc aagaacaagt tgaccacttg cataccattt
    13801 tccagcttga gttgagaagc ccaaatgctt gaccttccat aacacataag tatgctgatt
    13861 atctaaggtc aaggtttgtg gttctgcgta gacataagac ggcaatgcaa agactgccaa
    13921 tatcgaagca ataaaatgcc tgtaaatccg tttcatattc aactcccaaa ttaaattaat
    13981 gttttattta cccatatcca atgatccacc aacaagtttt tcgcaaatcc ctttgggtaa
    14041 taaaataaaa gcatcctttt ggctatccct ggttgaagac ccagcacaat ctgaggttgc
    14101 tgttgaacaa tcattcatcc cttttttaac cacaccataa catttctcct tactcaaatc
    14161 ctctgctatt gctgtgttgg atgcaatgag aactaaaaaa gcacttatgg tggattgtat
    14221 caatttatcc ttcattgaca tggataatct ccttattata ttgaacttaa ccagatctct
    14281 gcctataaag agataactct taagctgccc tgctattttt aataaagtaa gctttattaa
    14341 atcttcaatc atcagccttt ctttaatgaa taaatgaatg caaataatca tatgcttttt
    14401 tggcgtattc caaaggatgt gctttttccg gatcctgctc cgcctccact atcatccatc
    14461 cattatagcg atgtttttgc agccctgccc atataggtat gaaatcaatt gctccatcac
    14521 caggcacagt aaatacccct cttcttactg catccataaa gctgagtctc tccatttcaa
    14581 ctcgtctcaa gacctcaggg cgaatgtctt tcagatgaac atacaaaata cgatcaccgt
    14641 acttatctat taaattaacc ggatctattc ctgaaaagac agcatgtcct gtgtcaagta
    14701 atagagaaac caactttgga ctggtattgg tcattaaaaa atcaatgtct ttctcccaaa
    14761 acaccccagt tcctgcatgg taatgataaa cgatcttcat attgaagtcg taagcaattc
    14821 ggcccaaggt atgtagccct tgaatcaata aactccattg ctcttgagta aattgaggca
    14881 tatgagcact taatatcggt aaatcagtgc cttgaatagc atgaccgcat tcacaaacat
    14941 taatatacga tgcacctaat gcgcgcataa agcttaagtg ttcaagaaat cttgatacgg
    15001 ttttttcaaa agcattggcc tcagtaaaat aagtgctgaa ccatgaactg gataattgta
    15061 aacctcgttg ttccaaagca gtctttaacg ttcttacatc tcgtgggtat ttattcccca
    15121 actcagtgcc agaatagcct gccatagcca tttcgttaat acattgctca aaactaatgt
    15181 ctttacctaa attcggatca tcatcattgc accaattaat tggtgcgata ccaagttgaa
    15241 tactcatctt cacttccatc ttcaatattg tctaactgtt tcaatttgct ctcgcatcat
    15301 ttggtaagct ttactcactg attctgattg agatacctcc gcaactccaa cccgccacca
    15361 agtcttatat ccttgactca tggtctttgg taatacagga agaacaataa ccgtactatt
    15421 ttcctgtttt tttgctgagt ctaaagtagt ccggaattgc tcataggatt taacaaaata
    15481 agtagctgcc cccaatccct ctgcgtactt acaaaaatct atgggtaaat aatcgccatt
    15541 taaagcatta gtagttggct ccctataacg aaattcatta ccaaaaccaa gactaccatt
    15601 cccttcctgt aaatttctaa tgcactgaaa tccatggtta tcaaaaatga gaattgtaat
    15661 tttcttgcgt tcctggatgg cagttaaaag ctctgaatgc atcatgacaa aactgccatc
    15721 tccaactaaa acatagacct ctccttcctg attttctttt gcaatacgta ctcctaggcc
    15781 cgctgcaacc tcatatccca tacaggaata agcgtactcc aaatgataat cttttgcctt
    15841 tttggattgc caaattcgat gcaaatcacc tggcaaactc cccgctgcac taattatcac
    15901 atcctcttca gtaacatact gattgagcag tcccaaaaca gtagtttgat gtaacccagc
    15961 ctgctctgta gaataagact gagtcaattt atctaactct tgagcccatt cattttgata
    16021 atgacgtatt aactgctggt aagagagaga ggtttgataa tctgccagct ctttccccaa
    16081 ttgctccaaa ccagttttag catctccttt taacataact ccattcattt tcatggcatc
    16141 aagacgtgat acattaagat gaatgatttg gcagtcctgg tttttaaaag cccatttgga
    16201 agctgtcgta aaatcctgta atcgagaccc gataaccaaa ataacatctg cctgagctgc
    16261 cagagaattc gctatttcag atccagtaac acctattcca ccaacgttca tggggtgttt
    16321 cgctgccagg ctactttttc ctgcttgggt ttcagcaaca ggaatgcgat gttttaatgc
    16381 aaactctgat aaagtctccg aagccagtga ataatgcaca ccaccacctg caatgattaa
    16441 tggttgcttg gcctttctga tatgccgtat ggcctctgcc aacattgcat cacttatcct
    16501 ttctctctca atacgccata agcgcttttt aaaaaaagat tcctcatatt cataagcttc
    16561 agactgaacg tcttgaggca aacacaaagt aacagtacct gtttcaactg gatcggttaa
    16621 taccctcata gcatgcatac acgctgtcat taactgttca ggtctggtta tcctgtccca
    16681 aaacttactt actggtttaa agcaatcatt aacagatatg gtgtagtcat atggcacctc
    16741 caattgttgg agaaccggat ctggttgcct gcaactgaat acatcacctg gcaacaacag
    16801 taatggaatt ctattggttg tcgctgtcgc cgctgcagta atcatattag tagcgccagg
    16861 accaattgat gaagtacaag ccattatccc tagccgattt ttttgtttgg cataggcggt
    16921 tgctgcgtga gccattcctt gttcattatg tccctgataa taaattaaac catgagcatc
    16981 gtattccaat gcctcaccta atccggttac attaccatga ccaaatattc caaacacacc
    17041 attgatgaaa tggtattcat tattatccag aacgacatac tgattagcta aaaatcttaa
    17101 tagggcctga gccatagtta atttaatctt catttattta actctcaaaa tcatactgca
    17161 gataaggatt gttactgttt ctgttgccat gctcttaaat ttgccctgct tgtccttgtc
    17221 cattcatggt cttttgcgaa ggtaggcaag gtatatgcgt tattatttaa atgccggata
    17281 aaccacagtg tataaagtgc ataaccgggc gctgtacaat gagcatgttc ctgattattg
    17341 gcaatctggt aggtatcata gtgttcaatt cgcatcacct ctttaccatt ttcgccaaaa
    17401 gcatagcctt gaggctctga aaacctgtaa tggtaaattt caggttgttg atgataatgt
    17461 gaaggataac ttgaccaacg cccttgaaac gtaataattt ccccaactac caaattagat
    17521 tcgggtctgt ttcgtttatc aaataccgtt ctgacaagcc gatatgaagt attttcaaga
    17581 actccttctc ccctattgtc taactcaagc atattagatg catcaaataa aatcggaaaa
    17641 aataaatttt cgttttccgt ttccattaat aaaatttcac aatcagtcag agcgattact
    17701 gaacttactt cattctttga gcagtgcaag acatagggtg attgagaaaa ataatctgta
    17761 cgttcaactt cctggctata agaaccatac tggaaaacaa cttttcctgt cattaagagg
    17821 gctgcaaatt cataacttga gttaaattga tggctttgcc ccttatttaa tttaagagaa
    17881 ctaaaactca ttgctgtagg tgagtcctgc atcgttacaa tcgaatttaa accatcggca
    17941 aatccacatg gatttttaat aagcaactgt gtcttttgat ttttacttaa tttctcatgc
    18001 agttgtgtta actgagaact gctccatatt tcaggatcat gatcatattc ttgaataaaa
    18061 tagttcaatt cttccttgta tggaattgca ggagcgcaac catgacgagt cacgaccaag
    18121 gcgccacaag cattagcata ggttaccgct tgctcccatg atttgccttg cagcaaacca
    18181 gctaaaagcc cggacataaa accatcacct gctcccaaaa cattcaatac ttcaacagga
    18241 aaagattttg cctgtaatag acctttgtcc gccccaaaat gcacacgggc gcccttctct
    18301 cctaatttaa caactattgg tacatcagtt cgctgcctaa tattgattaa tgaaacttca
    18361 atatcattcg aaccgcctgc aatacatatt tcctcctcag tcccaacaat caaatcgcat
    18421 aaaggcaaaa cttgttgata cacttgagtt acatattcat tctggcaata acgactttcc
    18481 ccattaccaa tcacagttaa ccgccaaagg accggtctat aatcgagatc aaaaactatt
    18541 gtcgtttctg cttttcgggc taaatgaact acttccaagg tagtattttt cattgattct
    18601 gtggataaac cagtgcccgt tatcaataat gcctttgcat tttttaaatg agtaaaacta
    18661 atttgatccg attttatctg catatctgcg caatcattac gataaaaaag cagagggaaa
    18721 ttggagggag gcttaatacc caaaagtaca aggccagtta aatgattatc tgtttccgat
    18781 aataactcaa tattaactcc ctcacgttcc agttcttctt tcagaaactt acctaactca
    18841 tctgagccaa cacaggaaaa catctggctt tttaatccta atcgggcagc tccaacggca
    18901 atatttcctg cacaaccacc caaatatttt ttaaagcttg caacatcctt caaatccaat
    18961 cctatttgct ccgcgtagag atcaactgct acgcgtccca tacaaatcaa atcgattgct
    19021 ctattcctat caaaataaaa tccgggaaac tgtttcataa gggcctcaac tcaatactac
    19081 tgggagatgc gtttctaaag attgtttcgc tgcttttgcg atgcgcaagg cttgtagtcc
    19141 atccaatcct gaaacgggac ttgtctgatt atcattccag gcactataaa acgcattcag
    19201 ttctgccaga taagcttgat gataacgctc aaggaaaaaa tattgaggtt ttgcgccatc
    19261 cgttccatta gccgagtatc gagataccgt atgctccaac tgattttcag ccaataacat
    19321 accacctgaa ccgaatactt ctatcctttg atcataccca tagattgctt gacggctgtt
    19381 atctatgaca ccaaatgcgc cattagcgaa ccgtagttga atgattgctg tatctacatc
    19441 atcaaactgc tcaaaatcag gattaattag aacagcccct gttgcataaa cctccacaac
    19501 ctcggattgc attaaaaaac gtgccatatc aaaatcatga atagacatgt ccataaatat
    19561 tccaccagat gtcgcgcaat attccttgga tggacaagcc ggatcacgag aagtaattcg
    19621 aagaatatga ggagtgccaa tttcaccaaa ctgcactctt ttctgaatgt tggcaaaacc
    19681 aggatcaaat cgacgattga atccaagttg caataaagta ttattatttt tgattatgtt
    19741 taatgttttc tctatctctt cttcattcaa accaatcggt ttttcacaaa aaattgcttt
    19801 ctcatgaata ctcgctgtaa taatttgttc gagatgtaaa tgagatggtg aagcgatcaa
    19861 gatagcatcc aagtcagggt ggcatagcac atcttctgat tgagtagaca cgattgtaat
    19921 ccctaatttt tctgcccaat tcctatcgag ctggggatct gctatggcta ctaattcaaa
    19981 ttgcggtaaa tgaaatagaa tattttctgc atgcagtcta ccaattcttc ccgcacctac
    20041 tatcccaatt cgacatattt tctttttcat gattaatcca taattaaatt aaattttgct
    20101 atttttatat ttttcgatac tggttgacct atatctctta ctttttttcc agagcttaaa
    20161 ttttcttcca gtttttccaa cgatactgat gtagtttccg ggacaaaata ataaataaac
    20221 cagcatgcca gcaagcaaaa ggtagcgaac atagcaaagg taactgtttg acctgagaat
    20281 tgataaatgg gaagaaacaa aagtgataca acaaaattgg cgccccattg aaacactgtt
    20341 gcaatgctca ttgccaaacc acgtactgat aaggggtata tttcggatat taaaacccag
    20401 aacagagacc ccaggctaat acaatacccc atcacataaa atgataaacc gcctaaaatc
    20461 cagaatttat tgacaaacat agggttgcac gatagtacgc taacaaaata taagctaatt
    20521 gatgcgatca aagtaccact gattaacaaa aatcttctgc ctaatttatc aacataaaaa
    20581 agggtaacca ctgtaaaaat aaaattgacc agacccaaaa aaaaggtggc gagaattgca
    20641 tttttaactg gagaaaaccc aaatgattca aagacaacag gtccataata catcaaggca
    20701 ttgattccag aaaattgctg gaataggcct aacccaattc ctaataagag aacaaaaaca
    20761 attggttttt taaacatctg gatatacgct ggtgcaggat taactaaatg attactaatt
    20821 tcagttaatt cctgctgaat attatatcca actggtctaa tttgctttaa ggtctttaaa
    20881 gtagcatcag ctccgtattt catcattatc cagcggggtg aatgagggac aaaatacatt
    20941 cctgaaaata aaacgagggc aggtagactc cctatccaaa ataaatatct ccaactcatt
    21001 aaagaatagt catggagaaa atagccaatt aaataggcga cagcctgacc aaacgtgatg
    21061 gttagtccat taatcaatac taaagttccc ctctgatgag gtggcgcaat ttctgcaatg
    21121 aataaaggag cgatatagga ggcaatacct atgcaaatac cgataataaa tcggccaagt
    21181 aatatcgaaa cgaagccggg agccaaagca cacaaactac tccccagcat aaagcctaaa
    21241 gctactactt ttaataagga tcgtctgctt aacttatctg cgacaagacc acttatgggt
    21301 attcccaata tacatcccaa taaactactg ctgacaactt gcgcccattg ccagtctgtc
    21361 agtgccaatt gggagataac ctgatctttc acatcagcta taacgctgga atcaaatccg
    21421 aataaaaaac cgccaaatcc ggcaattacc gcgatcagga atacaaatgc gttcattccc
    21481 ttgttcatgt aactatctgt ccctaatgaa aattctgttc gtgcatgatg ttatcacgct
    21541 caggttgcga gaaaaatatc aaagtgtaaa ttattattta tttggtatta caagcagatg
    21601 ctccaaatcc atttataaaa tgggctatat caaatcgcga ggatttggca tcaaacatag
    21661 agtaagaata cctcttcaca ggtgatatgc tgttgctggg tagaatcccc tccattaatg
    21721 aagggaattt attagtcgaa tgacagtggt taattgacca aagcaataac aggacaaatc
    21781 actcctacct ctttaaaagc attgatgaca tcctgcttat tgtattgcct gtcaattgcc
    21841 gcttggataa caccacaaga ggctgcttca aatgaagtcc ctgacgtcca atattttata
    21901 ttggcatcta ccatcacttg aaatgccttt ctcactgacc agttcggttg atgggctaaa
    21961 agataaaaag ctctgttaaa gatcccgctg gtcagatgaa catccaagtc atcatcataa
    22021 tcatcgaaat gctcaataga atgaccatct ttcgttggat agtccatata acgaattggc
    22081 tcaccactta tagtagcctc acgccctata gaccaatctt caccatccca ataccatgga
    22141 aaatctttac gcaagtaatc ctgcatggca atcgatgcca tatccgagaa tgcttcattg
    22201 attcctccgg attgcccatt atagaccagg ttagaatgca atctggtaaa attatgagac
    22261 aactcatgcg ataaagtgct ctgtgccggt gctgttaatt gagtatcccc atcgccaatc
    22321 acaatttgtt gatgcattaa gtacaatcct tttattttaa tagaaggcac agcaaaggca
    22381 ttatcaaaat ttttaatatg tgtataggct cgcaaaggca agtcatcacc taaaggtttt
    22441 tcaacaccat aatactcttt gtacatttcc agtgtgactc cggcaaaata catagcatca
    22501 ttaattgaag aaaaactgta gtttgcaggt gatgtatctc catcattaag attgatatat
    22561 tgtgattctt tgctacaggg gtagaaaaat gctggtatat ttttatagat ttccacaata
    22621 cttagaacgg ggaaggatct cttgtccaaa tcagtatagg cggcattaat gacccttacc
    22681 tcagggcttt ctacaacaca attttttccc ttgatcgtta cctcaaattt gccaagagaa
    22741 ggaatatcct tcattgaatc accatgttga aacaatcccg accgataggg aagaatgaat
    22801 acattaccac ccaaaccctg accaactttc ttatgtgtta aatcatccca gctttttaaa
    22861 atctcaccag aattggcatc gataatataa ttcggagcgg agacaaaatg taatggatca
    22921 tttgcataca ttgataaatg ataagctaga taagcttttt tatgtgaatc cagataaatg
    22981 atttttttta tatttttgta aacgatcgaa ttgttattat attttattat ctggctttca
    23041 atatcagcag cacttaattt cccatcagtc tgatgaacat ccttttcaat ttcagacaca
    23101 tcgacaccgg tcagaaaaat ttttgacttg ctggtcttat gaaggattag ctcatgaccc
    23161 caaacaggaa tatttctata ataaacctgg taacgaatat cgttataatc acgattgccc
    23221 tcatgatcaa ctggtttcag ctgataattt aacggcgtga ttttattagt aattgacata
    23281 aatccttttt tatcttgcaa gatactaaca ttgtattttt ttaaattgtt atcgcccgct
    23341 tgccaaataa tactttctga agcagcttgc aaattagcac ttgccataaa aacaactaat
    23401 aatgaaacta tccgtgtgtt catatttttt ccctgtcttt gactattgtt gtgtcttaca
    23461 gcgtttattt atattcactg actcctgata tgtgttcgca tactgcatca gccatagcag
    23521 acgctgcttg actgctttcc ctgaaccaaa gctcaggttc tatcatttcc agctcactca
    23581 aacacaattt attattgtta tcccacatga catcaacacg tgcatacacg ggtaactcat
    23641 cgcattgagc tatgacattc tcaacaaact ccctttcttc ttgagatgcc tcataaggat
    23701 gtacagtccc accgaaatca tcctgtactc taaaatcgcc agattttgct ttttttagta
    23761 tagaatggct atatctaccc ccaaatacca taaaagagac ttctccttta tttacaatat
    23821 tctcctgaaa ttcctggagt aacatcgact cattgttaat taataattca aatacatctt
    23881 gataatgatc cgcagaatcc tgatcaaata aataagtatg gcgtgctccg cctgcaattg
    23941 caggttttag tatcgctttc ttccatcccg attccttcat taaattattt aagatattta
    24001 gcctccccct ttttaaaaat aaagtaggag ggatattaat tcctttttgc tgcatatcac
    24061 tcaaataatg tttatcaata ttccactgaa taatagaaat cggatttata aattgaattc
    24121 tttttgagca atcatttagc cagagatgaa actcgtcata acgatgaaaa taatcccagg
    24181 tggcacgaaa cagtgcatat ctagtctctg accaattata ttctcgatta tcccaatgag
    24241 ttcgtttaac ttttaatccc tttttctcca gagcttcggt taataatcga tcttctgtta
    24301 gcacattttg aatgtaggga gtaatatcgg ccgggtctat aaaatccttc tgtgttagta
    24361 ggacaatatc gtacttgtta gaggtcatga tagaagcaat ccgtatcaaa tgataaaaga
    24421 atacttatct ttttaaacta aatctatata ccaccatcgc aaatcaacct gctaatttaa
    24481 tcaaattaac aatgattgca ttttgaagac tctttgttta atctctggct gcgtgtgtat
    24541 tggaaatcac ttatttaagg acatgatgaa tgtttaaaaa tgctatagta agaacgccat
    24601 cttctacaat gatcaatgga ctaaccacat cggctgattt gggaaaaccc aattatgagc
    24661 ttgcattaat acagcatcaa aattatatca aagcactatc aacatgtggt cttgaagtga
    24721 cagtgcttcc agatctagaa caattcccgg atgcttgctt tgtagaagac gtgtcattat
    24781 taactgaaca atttgctgtc ttgacatgcc ctggcgctca gtccagacga gatgaagttc
    24841 aggaaattga acccagcatt caagcattct ttaaagacag gattttcaga ataaacccgc
    24901 ctggcagact tgaggctgga gatgtgctta gaatagacaa tcattttttt attggtctct
    24961 cggaaagaac taacaaagag ggggctgagc aactaattta tttactgaat cagcatggct
    25021 atacagcctc agtaattcag ctaaaaaaat ttctgcatct taaaacagga gtgagttacc
    25081 tgaataatga ttacgtattg gttagtggag aattaatcaa tcatcaagca ttcggtcatt
    25141 taaaacaaat agtagtaagt cctgaagaag cgtacgcagc taattgtatt atgattaatg
    25201 agactgtgtt attacccaaa gggtgtccta aaataaccca tcgtttatct gaactggggt
    25261 tttcaattat tgaattggat atgagcgaat ttaaaaagtt ggatggtggt ttaagctgct
    25321 tgtcgttgag attttaatga aaagccgctt atctgtttta agccttgcct taatcactgt
    25381 ttgctcggtt gatagtatta gaaaccttcc agcagctgct ttagcgggga accagctgtt
    25441 ttattacttc gcattggcct tgctattctt tcttctccct tgcgccattg ttgcagtttg
    25501 gttctccagg caatcagaac aaggtgtgta tgggtgggta aaacagggac tagggaatca
    25561 gtgggcattc atcgccattt ggtttcaatg tattcaaaat gttttaattt acccaacctt
    25621 gttgagcttt attgctggca ccctgctcta cagcatctcc cctgatttag ttcaaaacaa
    25681 aagtatgctt tttctaatta ttattttttt aatttggagt ttaacctgga ttaatttaaa
    25741 aggaattcat ctctcaagtc gttttaactc ttattgctcc ataacgggat taattcttcc
    25801 ttttattatt atcctgttcg tcgggttgta ctggtggatt acgcaggtta aacctggtac
    25861 cacagttcta ccaccagccg aatcttattc ctggacttcg cttactgcaa ttattttgtc
    25921 attttgcggt attgaattag ccgcggttca cgcacgggat agtaaacaag gggctttccc
    25981 taaagccata gctatttcag ttattacaat ctttctgacg atgttgtttg gttcgatcgt
    26041 attggctatg atcattcccc cagaacaatt aaattttatc agcagcattc cccagctcat
    26101 acaattattt tttaataaaa ttggttatgg atatattgcc tttattatta atggattgat
    26161 cgcaataggt tgtataggtg cagcaaataa ctggctcatt gcacctataa aaggattgga
    26221 atttgcaata aacgaagggt tcttaaatag gcaactcagt caagtgaata aacatagcgc
    26281 acctttcaga ttgttagtcg cacaagcctt ctgtgtatcg attattagtg ctttatttct
    26341 tatcgtacct tcgattaatg catcttactg gatcatgttg aatgcagcga cacaaatcaa
    26401 tttattaatg tacctcttgc tcttcattag cgctatcaaa atagtttcct caaaaccgat
    26461 tttaagtaag ctaaatataa tgattttcgc ttacctgggg ttggcaggca gtagcattac
    26521 tttaattgta agtttgacgc caccgccatc actcgccatt agctccaaat tgatttatgc
    26581 gttgcttggc gcaattttcc tggtcataat gaccttaatt cctatttgga ggaaacctaa
    26641 agttcttaca tgaaaagaaa tatccaatcc tgatttgcaa attcaaacgc aagtttcata
    26701 ctctcagcta taattgagat attaatcatt agttattgta aaatcaggtt aaattttagc
    26761 aggaattcag tactcatcgg agatgatgat gaaattgaca cggaatatga ttgctcaggc
    26821 tgtccaggct aaaataatta cagaggaaca ggccaagaac ctcattgaat tcataaaaaa
    26881 ccttcccgaa caaactccag gatttaactt aaccaatgtc ctttattatt ttggaggact
    26941 tatagctatt ggtgcaatga ctttgtttat gaatcttgga tgggaactat ttggaggctg
    27001 gggtattttc tatttgtcag tttgttatgc cctactcggc ctaggccttt gcagtcaatt
    27061 caatgggaaa ggatatgtca ttcctgctgg catttgcgct acttttgtca tttgccttac
    27121 tcctttggca atctatggct tacaaaaagg aatgggttgg tggccagatg acagcgttta
    27181 tcatgactat catgtttata tcaaatggaa ttggatcttc atggagttgg gtacgctaat
    27241 ggtaggtatt atcctggcct ggatatatcg ataccctttt atgattatgc caattgcagt
    27301 cactctttgg tatatgtcca tggatatagc agccatgttg ggtgacggtc aatttgattt
    27361 tgagctaagg gcaatggttt caatgtactt tggcttaatt actgtattga ttgccttttg
    27421 ggttgatgtt cgatccagtc aatcagcaga ttatgctttt tggctgtata tattcggtgt
    27481 catggctttt tggttcggtt taagttgcca atcctccgac agtgaaatat ccaaatttat
    27541 ctatatgtgc attaacctct tcatgattct gactggagtc attttaaata gaaaagtatt
    27601 cgttattttt ggtgcgttag gatgttctta ttatctgggg catctggcat tccaggtgtt
    27661 taaattcagt atgcttttcc ctgttgcttt gactgccata ggttttggta tcatttattt
    27721 aggaataatc tggcaaagaa atgaagcgaa acttactaaa gagctccggt ccatacttcc
    27781 taaaccatta agagacttat tggaagcaag agacagtatg gattaatgaa atattttcta
    27841 ccgtcctatc acattctatt actctttaga taaattgtag gtaactcttt actttcaact
    27901 acctgaagta gaagatagat gagttcatca ttattttctt tcaaaaggac ttgaggagta
    27961 attatgttaa ctttatcact tgccaaatgt attaatgcaa caaggaatgg ggcattcgcc
    28021 atcaaagatt tggatttatc aggccctcta tcttatgaag atgtattatt tttaactgaa
    28081 tatctaaaag tcacacccat aacagagctt gacttatcaa tgaccatcac cagggaaaac
    28141 tctaaagctc tatgggattt aagtcaggtt attgctaaaa attcactgct acaaaaacta
    28201 atatttgaag tgcaactgga cttcgatttc tattttaaac ataaccttcc aattggttgg
    28261 ttagatttta tagacgatta tcggttaaaa aaaatagcgg cgccactcca tgaggcattg
    28321 gttaacatgc taaaaaacaa accggctcta aataagctaa ctatagactt tttagcgatt
    28381 gaaactcctt ttcacaataa atatctcaac cagttagcac gatatatcaa taaaatcccg
    28441 cttaattatt tgaatttggg cctttcagtg accaaagaaa cacaagtacc gctatttaag
    28501 acgaaagaac cgaatcaaac tcttgagttc ctaatccttc gtggttttaa ttcaggtaga
    28561 aatatcttga aatatttacc ccaaacagca gccttaaaaa tgttagctct tgatgacatg
    28621 caattcgaag aacatgattt gaagttgctg gctaatatta tgaaatccaa ctcgtcactg
    28681 gttgctttaa tccttggtaa aactaatctt ggacaagtca attcgccaga ggtacttacc
    28741 tggctcagtg catgccccaa tttaaacagt cttatactca ttgataataa tctaagcagg
    28801 cttaatgtgg atagtttatg cgaattatta gcgaataatc tccaggcttt gaaaaaactg
    28861 gatctgtcca aaaatgctta tatgggtgac gatgcgataa agattgccaa agcgttaact
    28921 ttcaatactc atattgaaac attggtgctg gatgataact atattgaaaa tgagggacta
    28981 caagccatca tcgatctggt aaaaaccaat aaaaatataa cgtctctatc catgtcaaac
    29041 acctgtcgat atacaccttc taacgaaata attcacagtc tatgtgattt attacgtgat
    29101 cctggatgtc aattaaaaga actaaaattt aaccaggata ttactttggc acagtacctt
    29161 atgctgcaac atgccctaaa ctctaacaaa acactagaat acattgattt caattttaga
    29221 aatcagaata ttcttgaggc aattcttggg aaaagtaatt ccggtaattt gaatgaaaat
    29281 gagttttcaa gtgacaatcc taaagaagaa aaagccccaa atactttgaa tacggatccg
    29341 actagcaaaa gtgctccatt attttttatt tccccaaaga gtcggcaaga agagaataaa
    29401 aatgaacttt cttctttacc gagtcataga gtccatctcc attagggaca acacacatta
    29461 caatcaatta taatttctca ctggtaatgt acacagttgg cgctgagcaa tctgcgaggc
    29521 ccaacctacc ttaactcttt atattcgaat aaatacatta cgtttatata aaatcaaaac
    29581 aaccagaaaa ttcaggaaaa gaaaactgat agaatacagc aatgctgcat tgtgattact
    29641 aaatcctcca aaaaaataat ctttgagata ggatatcaat gccattttag agccatcagg
    29701 tgttgttatt ttaaaaacat attgcagttt taaaagaagg acatgaaata caaaagcaaa
    29761 taatgcattc atgccaaata ttttaaaaaa aacggaccat tttttaaccc caagtctatc
    29821 aatcagcaaa tagcaaaagg caaaggcaag cagagccagg ccgctggtcc agagtacgta
    29881 agaactagtc cataaatttt tattaatagg aaaactcata ttccataacc atcccaataa
    29941 caaaaatagc aagccaaccc ctgctaatag ataaaatttc ttgaattgat tgcatggatt
    30001 gattagtaaa ctaccggcca ggacacctga taaagtagtg gcaatagatg taaaagtact
    30061 taaaaaaccc tcgggatcat acgttttttc atataagtgg gaagctgaaa aaaataactg
    30121 gtcaaagtat gaaacccaac ttccgtcttt ggttaattga tttgctccat atcctgggac
    30181 gggtacttga gtcataataa tccaatatcc taataaaaga acaagaaaaa taaaaaattg
    30241 agttttaatt gaagtattca gataaatgaa agcggaaatt aaataacaaa cagcaattcg
    30301 ctgcagaatc ccatatatcc ttatgctatc aaattcaata ggatggggaa aaacattcaa
    30361 aaataatcct aaaagaaaca aaacaacact tctctctatg atggcagaat ataatgatgt
    30421 tttctctttt cgttccattt ggtttttcag gcttataaca ctcgttaagc cgacgataaa
    30481 caagaaaaat gggaaaacca aatccgccaa agtacaacca ttccagtcca catgctcaaa
    30541 aatcggatag ggatcaatag ctgcctggcc attaacgaaa atcatcaata caatggtcat
    30601 cccgcgaaat acatcaaggg ataaaaggcg ttggggtttt aagggaacag tattcatgcg
    30661 ctatttaaaa tggatttata aaaaactgag ccccattatc ctaccacgag tggcaaattg
    30721 taatatcaaa aataaaaatt aaatttctaa aatctatatg aaaatctctt ttaaacttgt
    30781 atgttagtca tgattgactt aacttgaagt tgaattaatg tttagtaaaa aagaacgtac
    30841 attattattt gccaataatt tgtggtactt gtcagaagga atgttaggtc cgctcttcgg
    30901 tattttttgc gaaaggttca actcgtctcc cttagacata tcatgggtat ggtctttgta
    30961 tttaatttca gttggaattg tggtaattgg cttgggttat atttcagatt ttattcataa
    31021 agaaaaattg ctggtagcgg gatatgcact ccaaaccata tttacctttt gttacatctt
    31081 tataacaagt aaaaagcaac tggctttatt gcaaattggc cttggaatat caggtggact
    31141 ctcaactaca acctggtatg ctctgtatgc ccgctatgaa gataaaacgc atgaaggact
    31201 cacttggggt ttggccaatg ggttatcaag tatttacact gggattgcct tgttatgtgg
    31261 tggtctaatt attgccaaaa catcatttga tgttttgttt atcatcatgg gatgtataca
    31321 atttctagca actatttatg tcagtaagct catctttatc gaacacccca aaattataag
    31381 gcgttttaga aatcctaatt tggctaaaaa ttaattttaa aatctgaaag ccatgttgaa
    31441 atgtcaaatc aaatgaacat tttttgatgt agattgatgc taaattcatc aagaaatact
    31501 ggaatcaaaa taacttgtct gttaatgtcc gcttttatta aaagaaatca cgtattaaat
    31561 tgttataagg atatgattat gtttgagtct tttcgaaaat gtttgtctgc catttctatg
    31621 cgagctccgt atgcctcgac accgggacat cgcaacgctc aacgcgctaa tgctggatcc
    31681 gaagtattgg gcacaacgct tgaaacattt gatcctgccg catcaattcc cacaaaactc
    31741 gctcaaactt taatctcaat ttattcccta ttcagatttg atactcatgt ttctgaaaaa
    31801 ctgatccatc ttttgcaagg tagtatagcg gcaacacaaa tgggtcttgg cattgctttg
    31861 ctttttactg gaacagagtg tgaggaatat acggatgctg atctatgcaa ggcaatattt
    31921 ctattacaat tactatacag aggaaccttg ctagtaggct gggctcctag tgaattttcc
    31981 aaggatccct atgctgagcc taaagttgta tcagaagaca aagcggaatc tgatgctgaa
    32041 tctgaagaag agaacgaaga tgaagagtca agaaactcag cctcagttta attttaaatc
    32101 ccttgctccg attaggagaa gggatcactt atatatcatc aaatatgcgt caactaacca
    32161 tctgaatagt aataccccgc gcatcagata aaccacggtc atctaccacc cttaccacat
    32221 atttaccagg tttcgcctgc cagagataag gttcatccga ttttgtttca gctacaaaac
    32281 tttcattgat aaaccagtat aaatgtgcaa tcccagcatc tgttaccgca gttaatggga
    32341 ttatcttgtt tgattgggta ttagcatgga caatataact tacccctgcc tggggagatg
    32401 taatttgggg gctaatacca ctatttcccg acaaaacgca acctggttca aaaaatggcg
    32461 gggtatggcg ttgaatccct gccctcctga atattcttaa taaatcggag ggccaaaact
    32521 catacacttc aaaacgagta ttttcatcga tgtgacaagt acgaagcccc gtcttcccgt
    32581 taattgccac ctctcgaaaa attgtatccg ttttaatagg ggatttacct ggaataaacc
    32641 aggtccattc tgtatccgta caatatcgac caggaagcat acctgaggct ttgcatactt
    32701 ctattttttc caaatgcatg tgttcagggt atttttcaac tcgagctatt ggtcctcttt
    32761 catgttttat tgcatcaatc aattcaaaaa ataaaggcgc agcaatttct ttacctataa
    32821 aagccggatt agcctgatta tcaaaatttc ctatccaaac agccaataca taaggaccaa
    32881 aaattccaac tgtccaggca tctctgtatc ctgatgaagt tcctgttttc caggcaattg
    32941 gtaattgtaa actgccatca ttattcgact ctttcatcgg agtatttttt agcatatcga
    33001 aaactaaaaa gcttgcttct gtgctaagca agcgtcgacc tttttgtcgc ggaacccctt
    33061 ttaatgaatt gattggatac cagaacccat cattaatcag catagcatat aaaccgacta
    33121 actctttcat agtgagttca acaccaccca aattaagaga caagccataa tatgattcag
    33181 aacgcaactg agttacatgc gcctgttcca gtaacttatg taatgttgga ttagttaatt
    33241 gattggaaag ttcgatggcc gggatgttac gactaagtac caaagcatct tttgctttga
    33301 tgggccccat aaaatcataa tcgaagtttt ccggattata gccattaaaa ctttgtggta
    33361 catcttttaa aactgtattg gggtgaatta aaccttgatc gagtgctaat ccataaataa
    33421 atggtttcag agttgatcca ggtgaccttt tggtttctac accattgatt tgtccactta
    33481 tcttgttatt aaaaaaatca gcagatccca tcagcgcttt agtactcata gtacggctat
    33541 ccacaagtaa tactgccgcg ttgtgaacac caaacatttt ttttcttgct aaataatgcc
    33601 ttgtaattcg ctcaactatt aactgaagcc tgtaatccaa agttgttgaa atactttgtg
    33661 ataaatcagg attttccttc agtaccatat ttacaaaatg aggagccagg aagggtaggt
    33721 tttgattggt ttgcataact aaaggcagtc caaacagagg ctgtttctcc tggtcttgcg
    33781 gatgctgctt tagccatctg gcaaataaac gatttcttat tttttttaat tcactgtttt
    33841 ttgatgtcct ttttactgga ttttgaggga taatgcttaa agttagtgcc tctggtaaac
    33901 caagtttact aacaggctta ttaaaataaa ttaagctcgc cgcccctact ccctctatat
    33961 ttccaccata aggcgccaaa ttgaaataag cctcaagaat tttatcttta tcataatgac
    34021 gctctatttg taaggcccta ataatttgcc ataattttcc agaaattttc ttggagttaa
    34081 ttccaaaacg aattcgtgcc acctgcattg tgattgttga agccccgatt ctgcgcgatt
    34141 taaccacata agtttgccaa atggctttca tcatggaaaa agggttcact cctttatgcc
    34201 agcgaaaata ctgatcttct tgtaaaagtg tggcctccat taattccttg ggtatttgag
    34261 ataacggaac ataaagtctg tatttgtcat cttcacttaa aattaatctt aataattgtt
    34321 gattatcatc atataccgct ttagaaaaat tgaattcttc taataaagaa ggcttgggag
    34381 aaaaaaataa taataaaaac ccactgacaa atagcgaacc caggattatg ctaaaatatt
    34441 ttattacttt tctcattgcc cacaacaggg atgatataaa ggttaaatta tattcaaatt
    34501 aatattgaaa tgcattagaa ttggcaaagg atagactaat aattaataat ttattgcggg
    34561 gggtgtatgc tttaccgcac cctcattacc agaccttaaa ataaacttag accaaccagg
    34621 actccatatc accatgaaca aaaacccatt ctccctaatt atgaatgtat tgtgttcaat
    34681 attttcaact ctattcggta aaataaaatg gaatagcccg acctggttag tttatttaaa
    34741 acacaaatca aaaaactctc cacaaatatt ttggggaaca actctattat ttatcgtgtt
    34801 attaggtgca ggagcatgtg gtgcttattg gtataataat ttgccaaaac caacttatac
    34861 tactgcatcc atcacaatac cagacatcac acctaatgag gaaaaccttg ttcccaataa
    34921 tctcattatt gatttcggta ttagaaataa cgaatttatc ccacaatcag ttgcccccat
    34981 taatttaatt ggcaaagaaa ttgatgaagg gatagaaatg gcaccacaaa ttgcgggcaa
    35041 atggagctgg aatagtgata gccaattaat ttttattccc gctgaagatt ggcctgcagg
    35101 gcaaaaatat actattcatt ttgcaaaaaa tttttttgca cccaatgcga atattgaaag
    35161 ttatgattta tcctttacaa caaagccctt tcaagccaca atccgcgagt tcaaatttta
    35221 tcaagatccc gtcaatgcag aaacaaggca cgctgtagcc actatagagt tcaactatcc
    35281 agtaaatcca aaaaatcttg aaaaaaatac ttctgttgta ttccaaacca ttaaaaatgg
    35341 aaaagcagac ctcactgcag aatccgttcc tttttcttat acttatgata ataataaacg
    35401 aattgcctat cttcattcgg caaatatcaa aatagaagat gtatcgcgtt atttactttt
    35461 aactttgaat aaaaacgtta gttcttcaac tgattcagca attcttgggc aaccaattag
    35521 taaaaatttg ctaatacccg atgcaagtaa ctttctaaaa atagtttcaa catcggctaa
    35581 tatagtccgt aatgataaag acaggcccga acaggtactt agtattgaga ccagtttggg
    35641 tgttaatgaa agtgagttta ataaatcagt acatgtctat ttgctcccgc agaattatcc
    35701 tgctaccgct gcagaaccag aaaaatccaa ttacgaatgg caaaatccag gtgaagtaac
    35761 gaataacatt ttgtctctgt cgactccatt acccaaagag gtaatccctt cggaacaaaa
    35821 ttatactacg ttgcatggct tcaaatttaa agctcaaact cccagatacc tgtatatcaa
    35881 gattgataaa ggaatgaagg gttttggtgg ttttaaatta agcaatgatt acacggctat
    35941 cgttccagta ccagaatacc ccaaagaaat cagtttcttg cataagggtt ctttgttagc
    36001 actgagtggt gaaaaaaaac tgtcagttct gatacgcgga gtgcctgcag ttaagtatga
    36061 attcgcgcgt gtgttgcctg ataatgtcaa tcagctggtt acgcaaaccc aaggcgattt
    36121 cagcaatcct tattttatta atccctcttt taatcaacaa aacatcagtc aaattttctc
    36181 ggaaatccag cagtatgact ccactgattt aaccagacag caatacacat ccctggatct
    36241 gggaaaatat ttagcatctg aagccaatgt ctcaggaccg caaggattgt tcttattaca
    36301 agcaacaggt tgggatgtag ccaatcagta tgctctcgat gttaaggcca gtcgtttgat
    36361 attgattacg gatctgggga tgatggtaaa agacaataat gatgggagtc atgatatctt
    36421 tgttagctcc attacacaag gactcccagt tgcaggggca acagttacga ttttaggtaa
    36481 aaatggcttg cccattttat ctcgtgttac agatgaacaa gggcgagcaa attttcctac
    36541 tctgaaagat tttattgaag acagagaacc aaccgtgtat ttggcaaacc tgaataatga
    36601 tgtgtctttt attccattca ataactacaa caggcaattg aatttttcaa aatttgatat
    36661 tggaggaatc tataccaatg atcaggattt gcagaactta acagcttatt tattttctga
    36721 tagaggaata tataggcccg gagacacagt gcatgttgga atgattgtaa aacgagcctt
    36781 tgctgaacca caaccttctg gtctgccatt acaagccact gtagtagatg ccaggggaac
    36841 tactatcaaa gaccaaaaag tgacattaaa tgacactggt tatatggagc ttgattttac
    36901 taccagtacc aattccccca ctggccagta tatggtttat ttatacttgg taaaagataa
    36961 tcacccgcaa aatcttctcg gttcaaccag tgttcgtatt tccgaatttc aacccgacag
    37021 aatgcgcatt acttcaagct ttgaacccaa accttttgat ggctggagct cacccactga
    37081 cctgaaagca aaggtagatc tttggaactt gtatggtgcc ccagctgcga atagaagggt
    37141 aagcggcaaa attttattaa ctcctcagcg agtacaattt gacaactatc ctgattatat
    37201 ttttgcggat ccactgtatg atccgaaaaa gcccccaaaa gtttttacag aaaatctggc
    37261 agaagcaaca actaacgaga atgggcaggc tgaatttaac ctgaatcttg aacgttttga
    37321 aaaagcaact tatcaattaa ctttttttgc tgaaggcttt gagcctggta gtgggagaag
    37381 tgttactaca caagctaaaa cgttggtcag ccctttaccc tatttcattg gctataaagc
    37441 tgatggcgat ttgcatttta ttaaacagaa cagtgtcaga aatgttaatt atattgcaat
    37501 taatccacag ttgggtaaag aagatgtaaa aaatttaaag atacaattaa tttccctgcg
    37561 ccccgtcaca actctggtta aaaaagctga cggcacttat caatatcaat ccacaataca
    37621 atcaacagtc atcaatacca atccattcga aattaaagaa tctggtgtta actacaccct
    37681 gccaacgaat actattggtg attactctct caacgtatta gataaggatg atagtattct
    37741 tagccagctg caatttagtg tagttggtac cagtcaggct cctctcgcta aaaacgcgga
    37801 attatctatc aaacttaata aagaggaata tcaagctaat gaagatatag aaatccaaat
    37861 cactgctcct tatactggct caggcttgat cactattgaa agagataaag tctatgctgt
    37921 gcattggttt aagacagaag cccaaaattc tgtacaaacc atccgcatac ctgctgattt
    37981 ccagggtaat ggatatgtga atgtggcgtt tattcgtgat tgggagtctc ctgagctctt
    38041 cattagccct ttaagttata gtgttgctcc attcaatgtg aaccatgata aacacgatat
    38101 aaaaattgga ctaaaaacac ctgaattagc ccgtcccgga gaagctttta cgattgatta
    38161 tcacacagac aaacccggta agatcatcgt atttgcagtg gatgaaggaa tattgcaggt
    38221 tgccaattat actacacccg atccacttag cttctttttc cagaaacgag cattagaagt
    38281 attaacccaa caaacagttg atcaaattct acccaagttc atccaggata gagaaatatc
    38341 tgctgttgga ggtgatgaag gtgaagcggc tttagcaagt cgcctcaatc cattcaagag
    38401 aaagaccgac ttaccagttg tttactggtc cggtattctt gacgcagaca gtactgacag
    38461 gcaattatct tatcaaatac ctgattattt taatgggtcc attcgggtaa tcgctgtggc
    38521 agttgctaat gactctgtgg gatctcgcga agtttcagca gaggttcgcg gtgattttat
    38581 tataagtcca aatgtgccta ctttcgttgc gcctggagat gaatttgaaa tatcagccac
    38641 tgtggcaaat aatattaaag attcaggaga acacgctcaa attgaagtag aacttagcgc
    38701 atcaccagag cttgaagtaa tcggtagtgc gaaagagtct ctggaaatca gcgaaggcaa
    38761 tgagcaaact gtgtatttta aattgcgcgc taaatcactg ttgggatcag caaggattgc
    38821 tatcactgcc aaatcaggag ataaatcaag caagatggat agtacgctca gcgtcaggcc
    38881 tgccaatcct ttcacaacat acattaatag tggcaaatca caagattctc aaaaatcgtt
    38941 agaggttatc cagactttat atccggagca tcgcaaggta gaagcgacta tatcgaccag
    39001 tccattagtt ctaatctttg gattacaaag atatttggat aacttcccat atggttgtac
    39061 ggagcaatta acaagcaagg ccctgccctg gctggttatg aacagtttac cctggttcag
    39121 ttcggaaagt ggcccaattc acgaaaaaat taataccaca attcaaatgt taagccaaag
    39181 acaaatgtct agtggtgggt ttagctattg gcctggtcta agtgacaatt cagcgaatag
    39241 ttttgcatca gtttatgcca tgcatttctt aactgaagca agagcacaag gtttcaacgt
    39301 ccccggagag atgttctaca atggcattaa ttaccttaaa gaattggcta gtcgtaatcc
    39361 cacagatgca gactcagcaa gagttcaagc ctatgccatc tatattttaa cccgtaatga
    39421 attagtcact acaaattatt tagccaattt acaattgtat ttacagcaag attcttcaaa
    39481 aaactggcta aaagacatca cagcagttta cattgcagcc acctatcaat tattaaaaag
    39541 ttatagtgaa gcgaatcaat tgattggcca atatcaaccg gcaacaaata atacatttac
    39601 gactgatttt tatgatcgca atatcgccga tgcgcaatat ctctatcttg tcgcaaaaca
    39661 ttttcctaat ttactgcctc aagtaagcga caatatactc atgaatttgg taaaagcgat
    39721 aaataatgat gaaatcaata cagtcttatc aggattttct agcctggctt tgggcgctta
    39781 cccacaaaat gaatcagcag agcaatctga taagctgtct ataacagagg tattggcaaa
    39841 caatcagcag agaaacatcc ctaccgatca aaataattat caaaaattaa atattgatga
    39901 aactgtcaaa acaatccgct ttaataatcc tgacaaacaa acattctttt atcagcttgt
    39961 tcagtcagga tttgataaaa taataccgac agaaccttta aaacaaggca tagaaattta
    40021 cagagaatat cgggatgaga aaggcaatgt cattgattca gcggttttgg gtgaagaaat
    40081 tgaagtacac ctgcaagtcc gatcattaga taataattat ctgagcaaca ttgccattga
    40141 agatcttttg ccaggtgggt ttgaggtcgt tagagactca gtaaaaacgg acaatatgga
    40201 ttatgccgat gtccgggaag acagagttaa cttctttggc tgggtagatt taactgcaaa
    40261 agaattagtt tataaaataa aagcgactaa cgttggtaaa tacatagttc caccagccta
    40321 cgcagaagcc atgtataacc caaatacgaa ggcaagaggt tcttcatcag tcattacagt
    40381 gtccagagaa gaagattaat tgctggcaag caaagtcaga ttttaattct tgactttgct
    40441 tgctaataaa tattaattgc cctcatattt gctcatgccg acaaatgaag gagatactta
    40501 tcatagcctg aggatgagat aagactcttt aggaggataa agactaataa aatgaacatt
    40561 gttgctggat attttccaac tctcgttgct tcaattccag ctcctgccta cttaaatcca
    40621 attgcttttc aagctgtaga gattttagcc tgagagcaat atgctcccta aaaaataacc
    40681 aatcatcttt ataatctaaa tagcgaagat tacgataggc ttcagcttca ctttggctaa
    40741 attggttatc aaccatatct ggtaacttat tagcatcatc caaagcattg ctaatgccac
    40801 aacccaataa aaaattggcc gacatcaaag catctccaac caagcaaaac ccacctccct
    40861 taggaaggag caaaacgaat ttatcagcaa tttcaatatc tgttttaaaa acagaagcta
    40921 aaccaggggc gctcgtctta aaattcgtat tgttaaataa taattgcaat accaagtcaa
    40981 accattgttt taactctttt ttattattat ccttgagaat ggattcagga atttccccag
    41041 tgagataaac agctccattc ttttttgaat atttcaaata aattaaaggt ggataatcat
    41101 gcgtccagcc catggccttt aacggagtaa tcttatctaa agagatgaag ctggttccct
    41161 cagtgggact taacaccgga ttagctatca tagccaaagg cggattattg atcgatagat
    41221 acatcacacc aaaagcattg tgatcaggat tatctaactt ttcataacca attgcgaact
    41281 ctggattttg cgaattccac aactgagtaa atgattttga tgtttttccg gatgcgtcaa
    41341 ccagcaaatc aaaactgtga ttttgtatgc taccattatg atccaaggtt aattcctgat
    41401 tctcagcatc aaccttggca atttgcgtgt ctcccaggtg aaaaatgagc tgctgctctg
    41461 cattattgaa ttttacagta taaggatctc cttcgtcctg aaaagtaaaa ccacctttgt
    41521 cataaatata tttcaactta ctatactgat aacgctgcaa ctctgcgata ggaattacag
    41581 tgcgctcttt ttcaattaat tcaaaaaaag ctaaatccaa ttgatcttct gcactcaatt
    41641 gcaagttgga ttcatcgatg ggacggatta atttacagat aaaattatct ttgaactcca
    41701 aggaataccc tttgcttgcc agttgactta gagcaaaaaa atctcttatg gtatcgtcct
    41761 ttaagaatac cccttgtttg cgactaaaaa catcatcttc ggatcggcgg tcaattaatt
    41821 caacggaata ccctttagct aataatgcaa gtgcactagc caagccagcc ggccccatac
    41881 caatgatata tgctttcttt cccataagtt ggactctact acttaaataa atgacaaatt
    41941 ttagcattta attgttaaga aattattagg tgagcatttc tattgccagt gtcctgttgt
    42001 gcaggatata actcagatag actctctttt tgtatagaaa gattagcatg ttgattatta
    42061 atttttctga actctgagct ggggggttcc aacttggaac ccctaattta ggttcccaaa
    42121 tcgaaacgaa ttacatccat aattcctttt tgacaaatat cgcaaaatga tgcgtgagat
    42181 tccataacac aattcaagcc tggtttatga cttgcaccct gatgagatgg tgaatctcca
    42241 taaccgccac gatatgcccc atattgcgga ggggttgact gccagtcact atagggggta
    42301 ggaatagaca tttgtggatt gactaactca ttccatttta atcccgaatg gcttggagta
    42361 gtcagaaaag tgatgttttg tgaccacggt tccttcatct cagaggcaaa ttccaactca
    42421 gtccgtccac caccttcata ttcttcattg agtccaaaaa aatgacccaa ttcatgtatc
    42481 agaatatagg taaaataagt tgaattatca gcaggtattg ctgtgtgtga tttgtaatta
    42541 cctacacccc agtactgact attattaact aatactattg gataatcgta aggtgctgat
    42601 gccaatccct gacgaaactt gttttctcgt gtaggatata ctatatgata ccatcgtcca
    42661 aaattatccc agtatggata atataaaccc agaaaagaat caaattctgg tactggtaaa
    42721 cctaagtctt cagcgactcc caaacgctta tgagatggat taaagactgc ataaaaatcc
    42781 atataatcga taccaggaaa ctgttgactt tgtaatgcct gcacagtttt aatcgctcga
    42841 ttccaaaatt tcatcttctc actttgagta tacccttcag catatatatt cactcttaaa
    42901 gagggagttt tcactgcggg taatagttgc ctcacttcca aatcattgaa ataacgatta
    42961 tctcttgtta attcactagc aatgatattt ttctcaacaa ccttttccat taaacctgtt
    43021 tgagggtttt ctgcgtaaag ttcaaagatc atatcatggg aaggaacggg gaaacgcaaa
    43081 ttaatagcac gtgccagctt gcgatattcc ttaccagtac caatcgcatc ataatagacc
    43141 gtatcaccac cgaatctgct ttttaaaatt cccaaataac tcccccattt gggtttgtga
    43201 atacttcgtg ataataatgc cggtgttcca gaggactcta ttattactgt accaacctga
    43261 taagaatctg tttcttcaaa ccaatcggtt tggattctta atacatcaat ccctgcattt
    43321 tcctgcacaa tcttcaaaga atcaggattg atgtaatgaa actttaaatt tgggtaatga
    43381 ctgtctctgg gtactttata gcggtcccaa tagggattgg cgtatgccag atgggaaacc
    43441 aaaaaaataa agcaaatgat aaaaaatctt atcatgggtg tgacctcctt gtattgcaga
    43501 actactatcc catactaact tgcagatttt cctacaaatg ttaaccgagt ttaacgaaca
    43561 ctcttttatc tatcgaactt gatatttcgc cagattgaac gaaagcgtgt taatttttag
    43621 gtatcgtaac cacgactcac aaaaaatcat gatttataag acaaaagagt agaattgccc
    43681 atgtcaccta tccatggcgg attatcacct aataaaacag acggatcaaa acctaatgat
    43741 tcgattctaa cccactcttt atccaaatat ttcaattgat catttaacgc tttgttatct
    43801 gttttatttg ttgcatcata gcaacgatca aaaggatcgt aggtataggg attgcaaact
    43861 accgcctcca ttttatccat tatcccaatt ttgttatacc aatcatcaat caacattgga
    43921 ccttctggtt tggcagcggg atcgagcaca taaatggtac ccatgtagtt gacaacagct
    43981 gacacatgat accaccaact tactgttccc tcagaagagt atggtgtttg catttccaaa
    44041 tcaccaaaga caaaaatttt tgctggaata acaaaatgtt cactatccag tttaattctg
    44101 gacagtgctg ctctggcaaa gcaaccatca tcaggataaa gccaactgat tcttctttga
    44161 aaatcaggtt tatcaggtga atacaaaaaa cgagtgtctc gtatcaggtt aaacatgttc
    44221 gccaactcct cataactgct aacctttggc acagttgagt aatctatttt ctgtaaagag
    44281 actttctttg aataaggagt ccccctggca attaacatct tcagttcatt ttgtggttta
    44341 ttatttatga tacgtttcac tgcatcctga taagattctc ctggaagccg tttttgtgag
    44401 gcagtcgcgc ttgccaagcc gcaacaaatc aaaaaaggaa ataatgataa aataatggct
    44461 atttttttca tagcaatcct tgagtattta taatattggt tgatatttta ccaatatttg
    44521 tcgtccttga cattctttat aattagcatg aactgattga aaagactagc ctattagtca
    44581 tctgaactat cagtagtcaa aacatatcag gtcaaaacat atcaggtcaa aacataatga
    44641 cttatatttt ttgtgagggt aagcattcaa aatactgaat ttaattgaac tgaccttaaa
    44701 ataagtatct cataataatg atctataatt cagatataca tcaatttata gattaaaggc
    44761 tagtcatggc taggcaacgt ccctgaagtt tttttagaca aaatttaatc atgaattgag
    44821 ctcggatttt caacttcacc aggatcttcc ccctctcccg cgctaggagc tttgatagta
    44881 tgatggtgcc tttcgcggga gagggttggg gagagggaat gtcacggatg gtcattgatg
    44941 acgagatgtg gtctcgttta gaaaaattac ttccacagcc taaaggtcgt catggtgaag
    45001 atgatcgctt atttatggaa gcgatatgct
//
LOCUS       DBNCWK010000015        40819 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.015, whole genome shotgun sequence.
ACCESSION   DBNCWK010000015 DBNCWK010000000
VERSION     DBNCWK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 40819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40819
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.015"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..759)
                     /locus_tag="AC06IO_000684"
     CDS             complement(<1..759)
                     /locus_tag="AC06IO_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HIO9873897.1"
                     /translation="MNDMWYQNIMRNLLEKLPEASRKDGEFFLDKLFLAVKRVDNKPE
                     MEFFLGLIEKSFLQFENEKSGSVTLVKFSRHILGLTLLYIKSGNDSAIWNSDFIPFLG
                     EIKKFFDVEKEGKPFYKFLNQLEVDVFVSLNHRLAIDFKHFSSILRGNNDLKTIQSFT
                     EACKGLGNESSEGFSQVMKDAEAHMINLQFANDLNSGFNLEGIDENLSNTTLQKSSHV
                     NSMSMSVNKGHRLSIFKALSTSGELSDQTSLFINQ"
     gene            complement(842..2164)
                     /gene="gabT"
                     /locus_tag="AC06IO_000685"
     CDS             complement(842..2164)
                     /gene="gabT"
                     /locus_tag="AC06IO_000685"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HIO9873898.1"
                     /translation="MKKIYIKTPIPGPKSQQLMELRRQHVARGPFHATPIFVKQAKGS
                     FVEDVDGNVFLDFSSGFGVVNTGHCPDSVVNAIKLQAEKFIHTGFNIIPYESYIKVCE
                     KLNDHTPGHFEKKSLLLNSGAEAVENAIKIARAYTGKQAVICFDHAFHGRTYMAMTLT
                     SKNKPYKHGFGPFPSEIHRAPFPYEYRWKGANCVEECFNEFTDLANFRVGVENIAAVI
                     IEPVLGEGGFIQSPALFLQKLREFCTTNDIVFIADEIQSGFGRTGNLFAMNTLGVPPD
                     LTISAKGLGGGVVLAGVTGKAEIMDAAMEGGLGGTFGGNPLSCAAALEVFHIFEEGSL
                     LQNVTHLAKALQSRLSEFKEKYKVVGDVRGLGVMQAIELVKDKNTKEPNKEAAVQLAQ
                     FCLEHGLIILTCGTYGNVIRLHMPLSTGVKDLEIGLSIIEEGLKKLSV"
     gene            complement(2169..3635)
                     /locus_tag="AC06IO_000686"
     CDS             complement(2169..3635)
                     /locus_tag="AC06IO_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIO9873899.1"
                     /translation="MEIYEMYIDGKFTLAKSGTTRDIFDPASGELIARVPESAKEDAI
                     LAIKSARKAFDEGEWRKSLALDRGKLLFKLADLIRANAKMLAELETRNCGKPLPEAEF
                     DVTDAANCFEFYGGLATKIHGETMSVPANSFSYVVREPIGVCGQIIPWNFPLLMAAWK
                     LAPALAAGNTVVLKPSELTPITALELFKLIDQCGFPAGVVNLITGPGIGVGEELASNA
                     MVDKVAFTGGSATGRKIMQAATGNLKKISLELGGKNPNIVFADCDLEMAIDGALFGAF
                     ANQGEVCSAGSRLLVERPIHKKIVEGMLKKIPNIKLGHGLDAGVKMGPLVSSAHREKV
                     ESYVKLGMEEGAKLLCGGKRPTGSEFAKGHFFEPTIFDEVKSTMRIAREEIFGPVLVV
                     IPFDTEEEAIRIANDTDYGLSGAVWTKSVTRAHRVTSQIRAGILWVNHYHPTYNEMPW
                     GGYKQSGAGRELGLYGIESYLEVKQVNINLDETPIGWY"
     gene            complement(3652..4446)
                     /locus_tag="AC06IO_000687"
     CDS             complement(3652..4446)
                     /locus_tag="AC06IO_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIO9873900.1"
                     /translation="MATLKIHGVDIYYELYGQGKPLVLIAGYCCDHTFWNAMLHELAK
                     EFQILIFDNRGIGQTRDDGGSFTLEAQADDIMAFLEQLGFRNPSILGQSMGGAIAQLL
                     ARKHGKKISKLVILNSVAKFNTRANQAMESLLNLRKENISFDLLIEAGIPWFFSSEYL
                     AEPKNIATFKENLKNNPYPQSLQDQARQFRSIPPFDSRGWLHEIKVPTLVIAAEDDIL
                     TLPAESQQLAQGIPNAQFITIPGGHSSPLEHPALVNDAILKFLTSS"
     gene            4728..6707
                     /locus_tag="AC06IO_000688"
     CDS             4728..6707
                     /locus_tag="AC06IO_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873901.1"
                     /translation="MRYVNFELLKRIPQHLKGVMEYYPDVLLFQLNQIQYTHLWHELS
                     SAKYLYTNGFVIRPTHWLMYAFQTVKGWLGFDNHCQPEKISYVLDKLSYYGYTKQFLQ
                     PDFSSITNFSISPEISALVVKSRDDLTTAQLQNKLVNNYFKVEPHLGIDYSYQKLNPN
                     HRFGESWMHAHEWGLIPQLDPQDDSLIAEVISTLDKKGISAKDVFFLQHSKYAKAAAQ
                     YYCDKAKNTIVPSFFFRLLWTDPRPRYLALALAYDPEIAKRDAQKFIEYHLAQKEYDN
                     AFNLLGILNDSNLVLKFLLAIPETERHALIQKDTAIAAIMAKYYIEKKQYFLATQFYT
                     NIEEISPNAAFAIEIQEQNYEKAYDIFKKYNSPDLFSMPERKLLAKVFYSEAETAYVA
                     GKTYRGNKSWEKAKQYYLQSLEQKKAAHHLDPSDEYLEDLYAHKRLYALLLIDADIDL
                     HKAEDSDIASIQKAITLLRECRSNNKEEQEHHKRALAAGLMRRVDTLREKIAFNYYSS
                     DFESIRKHKIEHQQDIAILIKTLGELIALLEGTQDKALRLQLGKAHYLLADVQCFFDI
                     NAPDINQHYKMAMKAVPGNPFYVLRVAELFEEEKSKLQKIGITQLKNMGYQVFDFLHW
                     SEERWCKRDDIIHNIKDIHMPPSEPINSSSWNFTF"
     gene            6802..7284
                     /locus_tag="AC06IO_000689"
     CDS             6802..7284
                     /locus_tag="AC06IO_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIO9873902.1"
                     /translation="MATYYGQCACGKIQFMCTGEPVFTQYCHCNKCREIASLSQKKQD
                     KSGYSLTAAYLTRDFRIVSEENDFEEIIKEKAKLFLCSYCHSLVYGIALDPAQQDSIG
                     INVNNFSFDKSIPDSFKPVRHIWYASRIMDCDDQLPKFKDAPKEQFGSGELFELPNAN
                     "
     gene            7704..12194
                     /gene="sidE"
                     /locus_tag="AC06IO_000690"
     CDS             7704..12194
                     /gene="sidE"
                     /locus_tag="AC06IO_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SidE"
                     /protein_id="HIO9873903.1"
                     /translation="MPKYVEGIELTQEGMHAIFERMGHPNITSGTIYNGEPTIDKGAL
                     DRQGFMPVLTGVSPRQDSGHWIMLIKGQGNQYFLFDPLGESSGKYYQNILAKKLPGAT
                     LSVIPNNAGLNMGLCGYWVASVGLRAHAALTQPIPPSLRNVGQTITQEMRDELTQDGS
                     EKITRWLRAVGNEFPDGDIQPDATALRRATEKNVRIDEFQPVLTGTSPKEISINPTAP
                     QEVSVPTWNGFSLYTDETVRNAARYAYDNYLGKPYTGTVEATPVNFGGQMVYRQHHGL
                     AHTLRTMAYAEIIVEEARKAKLRGESLKTFADGRTLADVTPEELRKIMIAQAFFVTGR
                     DDEESSKNYEKYHEQSRDAFLKYVEENKSTLIPDVFKDEKDVKFYADVIEDKDHKWAD
                     SPAHVLVNQGHMVDLVRVKQPPESYLEYYFSQLQPWIGSTATEAVFATQRQFFHATYE
                     AVAGFDSENKEPHLVVDGLGRYVIGQDGNPIREESDDEDEEESGELKFFSQKKKLEEN
                     QRYMRVDEYLKLDEVQKRFPGAGKKLDGGLPGLKEYQYLQRLNSINRARCENDVDFCL
                     GQLQTAHHQTKITPIKRAFQSSSDKVRRQPNMDEIAAARIIQQIMANPDCIHDDHVLL
                     NGQKLEEKFFRNLLAKCDMAIVGSLLNDTDIRNIDTLMQHERNTEFHSTDAKAKPVKL
                     GETWEKTIRSGGGVTQIKHDLIFLMQNDAWYHTRVNAIAQNRDKDSTFKEVLITALMT
                     PLTNKSLIDTSRSPAPKTLFRGLDLSEEFKNKLINQAETIIANTTEHLFTDLSTEAFK
                     QIKLNDFSQVSARTCASTSTNIEVPRTIFGSNTIFEILDPDGLLHPKQVGTHVSGSES
                     EYSIYLPEDVALVPIKVSFDGKTGKGKDRHIFTLVAVKSPDFTPKHESGYAVEPLLKM
                     QTPKLEEIQRLVEQAREEPDLERVFNLQSRVARQARLHSTESGYKTFLNERVAPVLEQ
                     SLNGLLDNNAVTLGKVLSAFPSDGQWSAFNSVEARQMKIQMDAIKQMVEKKAVLEGQI
                     LPALAQCQNALEKQNIAGALQALRNIPSEKEMQTMLSISGGLRGQIQRTKQDLTETLE
                     PLQRAITAKLVSEQEKVKVRYEKLIASIPQRIADLEKAALSDLAGVKKVVSHFNQLQE
                     ELKLLRNEKIRMHTGSEKIDFSDIAQLEAQLQKIHTKLYDAYLVELTKEISALVKEKP
                     KNLADVKRMVSNFYAMSADIEQLRQEKIKEHSESKDPIDMSDIDKLKEELQKINQFLV
                     KAMGTNIRVSLNQMEVKTFDAQEKEAQQNLKQLDALINKLESSHAVQKQKEELEKLNQ
                     LLVEKRKAYPAMVQLQFRSEALIIHLRELCEAHQAQMAKTRNVRAQEITNGRWKVQWL
                     TDWVGLTTDERVTLANKEKELAKFKEDLNNDEYDLQELISNLAEKNPSELEEAIGISK
                     ESAQKLHKLLTHLNHSTTFMNKIDQRLQSIDELLNEFGKQSPRTEMIKTVEEKQGTLL
                     RL"
     gene            12493..14085
                     /locus_tag="AC06IO_000691"
     CDS             12493..14085
                     /locus_tag="AC06IO_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIO9873904.1"
                     /translation="MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATY
                     FLTLQESSTVGIAAGYAMITRKPALINLHNYVGLANAVCNMYNAFTSGIPLLIIAGQH
                     SRPYLIHSPVMSGDLTSLAHTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
                     PMDIWQEETQETTIKKTRFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIR
                     LLHTIADQLNADIYAAPYHVREVINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEK
                     VKGYLYTGQSSIPQGIRFIQISAASEHLAFDYPCDLAILGNLEATLTAISKHLKINGL
                     NVPLKSATNIKNLEKKYKQHPMHSLIVTLLNQVNRSVHLITEGSSEDAIVQDIATTLG
                     FLNVNFSPRGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVI
                     FICFVNREYRVLKTDMSQNRSFTEEDFFRLDLNHPPIDVQQIALSFGAQVKTIKSKEE
                     VETVLKSSFAFQGPTFITLYHQ"
     gene            complement(14202..14648)
                     /locus_tag="AC06IO_000692"
     CDS             complement(14202..14648)
                     /locus_tag="AC06IO_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIO9873905.1"
                     /translation="MKHNLLDKAYKHCVNHGYRFTEPRERVLKILVDERKPLGAYDIL
                     QRLSMEVDNPKPPTVYRAIQFWHQEGFIHCIDSLKSYVACLHGHHVGQAQFLICNQCD
                     FVKELECVVDFTAVTEAANSIQFSIINCTVEIKGLCSDCNLTNLKN"
     gene            14979..17537
                     /locus_tag="AC06IO_000693"
     CDS             14979..17537
                     /locus_tag="AC06IO_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIO9873906.1"
                     /translation="MLYSNYRFYLAKDAQLLNPEESREAIEQILAKTKELELIRVAVD
                     LTSSNKSISLLSASKENHVIKTKVTQILKEQGLSVIDMMEVEVLQDIEKIENRHPTHK
                     SKNTHLHPIKKHQKKAKHDHSHGHGHHHGHHHENHWIKAALGLIWGIGLLVLSIGTFN
                     IPLVAYYVITGLTSLMTLYLGHTVYQSAWQALKEKKWDTTTLYSISTLTILAVSIASV
                     FVPGLPMMFEAAPLVLGFWHLGEGIEHTLVGKINKKLDVRDCVPPLVLLKGNPDKEIS
                     VKTLIPNDMIIVKKGQVIPVDGVLTQKALLYTTRIDGSPKLKEFHPGDVVKAGMQLAD
                     HIPSLEMQVTKTYQNSYLSLIAENINKANDEKAPIEMFANKVLKYFIPGLIAVALLSG
                     IVIGSLFNPALAIQCVVSVLVSACPCALSLITPMAVKIGMKKASENGIHFNNGKALQA
                     AADIDTVVFDLNGTLTQGKMLVKSLHITDKKYLRPIALLESQSDHPVAKIIKSYIEQQ
                     DTASTEPLEITSIDKSHHSGMKGIINGETFMIGNKDMLAANGITSIDKPYDNPEKGSV
                     YIVRGTTVIGQIALIDPLREDAIATVKQLKRLGKSVHICTGADQATAEKYAALLGIPK
                     DNICANTVGAVTQKGEVSKTSYIQQLKQKGFKVAMVGDAANDATAIAYSDIGIAVKSS
                     IGDSITEQHAGIVVQQGLLFPIATAFDVAQKTKQNIFQNLFVSLSYNSIITLVAAGLF
                     VALGFALNPVLGVALMVLESTIVLTNLYLFKQQDIVSSTSSNANNAVEVEPTANTTSK
                     VLHALGHRSQPQENLAIVTESKPVNPKVINLFAPKEHPSYRSDVTQQKLGCGIC"
     gene            17680..19809
                     /locus_tag="AC06IO_000694"
     CDS             17680..19809
                     /locus_tag="AC06IO_000694"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIO9873907.1"
                     /translation="MNSTWHQTAAGNRSILSNLRLSRTLLNVIFIIALSLLLIINIIS
                     YNQVKNLIFASNWVSHTHEVIQTIDTCLYDVVEIESRQRFYLIRGSSPQFMTDVDDMK
                     STLQLNLDKLEQLTRDNPEQNKRTHRFIDLVKQRLNLLSQLVQLKGSGKLDTQEGYDL
                     LNQSQDMSHQVKSLGQEIKSVELVLLSERNTGAVRWADTSSFILISGGITSILFLTIA
                     FILANIELSTRRKTELQNQNTQIRLKKIIESASDMIAAFDKDQRFITFNEAYQREFKR
                     LFDKSISINMSLEEAFDDVPENKKKLVQTWKESLQRDEETKNIEVNTEQEKTIYEMTS
                     KLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKKLQEKNEQITLLVEMS
                     DIMLACGSQEELSDVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPQPHDLTFT
                     PEQCWAIRLGRIHYAGSSRIELMCSHTMFAEQPELSLLCVPLMAQNDIYGLLYLEVGL
                     KFDENQQLLITAFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFLFKQLHQ
                     AERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNSDIRLGDIAARYGGEE
                     FVLLLYDIDAQAAKMKAENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEV
                     IEAADKALYQAKNKGRNKVILFSEISS"
     gene            20021..20854
                     /locus_tag="AC06IO_000695"
     CDS             20021..20854
                     /locus_tag="AC06IO_000695"
                     /EC_number="1.14.14.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004392 - heme oxygenase (decyclizing)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006788 - heme oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HIO9873908.1"
                     /translation="MFSKALISATYKNGKPGQLTEEHEKAEHHRFKTDYLFKNAEISK
                     DLYASRLIQHFLIIKAIETKLQNLPKTEQSEISAFFALSYLEHLWRTPAIENDLRQLN
                     INPDEIHKDVITKTTENYLENIEKFTPKILLAHFLLHIAGFMHGGNIIRSKYIEPSNR
                     LTTYQITADQYDFSAAISFLSSGKHSPLALYQDMMKQIDNIVLTGDEYQELLEQCKGI
                     YETMSNIYDDLCDMHTHHLKLPTYSLAAISVSLIALGLILKLLIDCLNPMTNPLSNAP
                     R"
     gene            20906..22471
                     /locus_tag="AC06IO_000696"
     CDS             20906..22471
                     /locus_tag="AC06IO_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIO9873909.1"
                     /translation="MLKRDISTLNILVASAGGMIGSGWLFSPFISAQMAGSNSLISWV
                     IAAIFMLFIALPLCELGTMFPVSGGMSNYPTYTHGREVGFLFAWTSWLSYVVMTPIEI
                     QAILQYSSHFFPILIDNHSESLTLSNYGYLAAIGIMFFVVILNSYGIKFLAECNKYAS
                     VIKFVLPSIAIISLLKQSPSLANVNINLSSKEHWVEIFTALSAGGVAFAFTGFQNGLI
                     LAGEVKNPQRNIPIAILGAVLVGFILYFMLQFSFIAAVPQKYLAQGWHALSYPGDEGP
                     LVGLTLLLGLGIVATLLLFDAAFSPFGTTLVYTAATSRILYGMALNQHLPKFFLKVNR
                     HNIPYVTLYANLLVGSFAFLPFPGWQKLVAFLSSASILSYGIGPICLLAMRKMHPHMD
                     RPFKLANSIFLSHIAFYICNLMLYWCGFSIIWKLNLALLIGLGIYFFYHRHISLRNGS
                     SLVWFIMYMGSFSIISYLGSFGGIGLLKFPFDLICFLPYSILILYMSQVLATPRHDND
                     MIGNVTELPVYNS"
     gene            complement(22642..23748)
                     /locus_tag="AC06IO_000697"
     CDS             complement(22642..23748)
                     /locus_tag="AC06IO_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HIO9873910.1"
                     /translation="MSYSIDNPGGGDCGFYALAIGLISIIQNEYKLHGKSKTYDRWKQ
                     VEGIYGVRQQDILNVDLTKLHDSPYTYKSELLFLLQMSLRNIAVIVNKEDLVKRIIEE
                     GRSKDQTKIEGSNAYCKFMELVQLYLYRRDSLARISQFNELALSPEIVQTAQDIARIL
                     RPILRNQPFHQVQKIENAFVKEVLLNDVLSGNTINSNSIILKNLEKIKEQGRWATHDD
                     LKEIADLLKINLNVVGQPNGKPIPEYPTVTLKNLSNTHWITQVESLQVPRQYSTPRKR
                     AFGYETESDRVVPLKEEQTLDRAFRTETQSERTKRYRQHFETLFDTLSPYGFFTYEQN
                     KRNTQVSGKVQTGLEFDESLAAQLQEAELRRILY"
     gene            complement(23931..25328)
                     /locus_tag="AC06IO_000698"
     CDS             complement(23931..25328)
                     /locus_tag="AC06IO_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIO9873911.1"
                     /translation="MICFPSGKNKLVVLCLLLMLSFKSFPIPGKPLLLDAVLKSVDRS
                     YPQIVIARLEIRKAEGDYVNALGKFDPSFDVNPRFQPVGGYINNYADNLFNVPTLYNG
                     LKLFGGYRIGRGDWPIYYQNYLTNSGGEYRAGLSLPLLRDRRIDKERTELFTREETIR
                     MKQQDAAATKIKIYQETIKAYWQWVEAGFQLRTFRHLLNLAKERQNAIIQQAQQGDLP
                     RLAIAENLQLIVQREQLVNQGEMLLAQAGINLSLYYRDAKGKPQKPDESQLPATFPAQ
                     PIRVSNSISQLQRHPVLRKLENYSKIVKLKQNLARNELLPNLDATAYTFKQYGTGGYP
                     LLIPQAAMIGVNFKFPIFQREARGKLISTTSELQQVKTEMKFTYEQLNNELSNLLVAI
                     RIYQQQVVLLNKELNLARQVEKGETKRFYEGDSTLFLVNQREQTTAQVKLNWINAEVN
                     LQEATALARFFSSTQ"
     gene            complement(25325..26311)
                     /locus_tag="AC06IO_000699"
     CDS             complement(25325..26311)
                     /locus_tag="AC06IO_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9873912.1"
                     /translation="MKLHAYSMISKLPKPQKIAKIIFIVFIGIILFLSFTPWQQFALG
                     NGKVIAFSPTERQHTVNSPVSGRIKKWYVDEGMRVKPGDPIVDITDNDPELLSRLEVE
                     KKAILLRIEAAKQAIAAGKANVDRQKKLYEQGINSRRQYELAQIEYAKYQNDLAQANI
                     DKVNVDVRIARQKTQIIKAHVAGIIFRRLTGQESVVVNAGDVLAQILPETESRAVELW
                     VDGNDIPFVRLNQKARLQFEGWPAIQFRGWPEIAVGTFGGTVSFIDPTDNGFGLFRVV
                     IVPNEPWPSVRFLRQGVRVHGWVQLGQVPLWYELWRQYNGFPPESDRENKAS"
     gene            complement(26308..27897)
                     /locus_tag="AC06IO_000700"
     CDS             complement(26308..27897)
                     /locus_tag="AC06IO_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIO9873913.1"
                     /translation="MNAKPINSIIDVLDRQTISSIAMISVIISLLSLSIPIAAQTLVN
                     LIAFGKLLQPVITLSLMVLILMMALGALHIWQIVIIEVIQQKLMVKVSLSLTRHFTHM
                     SLDNFSTHHGPELVNRFFEIVTIKKSLASLLLYGINLGLQLFFGLLLILIYHPLFLLF
                     DIFIILGILLIIFIPYKKALESAKKECSEKHVVGAWLEEILINRYLFRFNLHHRYVTQ
                     QTDKKLVAFLKARNTHFKQLIKHQIGFYTLSALASSLLLGLGGYLVINNQLSLGQLVA
                     AEIVLGALIYSFRRFGALLENYYDLVASENKIDAVLNLSLEQVKEDEHGELLTPINSI
                     QLEMDSKNKALIAVDKPLLVFSEKSDLCQLFVEQLFGFQDPVSLHVLINEIPCAKRTL
                     ISLRRFSLLIAEPQWFTGSIYDNLVLNHRNMSNKIIIEQLQNVGLADKIIQLPEGLHT
                     IVYEWQDVFTSVELTKLMLVRALLLQPELIVIDRAFDILNHQEVNEMMELVLSLKNTL
                     LVIVSQRSDFNYLNNRLVISS"
     gene            complement(27939..28790)
                     /locus_tag="AC06IO_000701"
     CDS             complement(27939..28790)
                     /locus_tag="AC06IO_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIO9873914.1"
                     /translation="MSIDTITLQCFLAVAETQSFTKAAQRVGRTQSAISQQIAKLENL
                     IEKPLINRGRELSLTTDGEIFLGYAKRIYELHRESLDRFKAPELQGEIRFGLPEDFAS
                     IILSDVLVEFSRLHPRVLLNVECDLTLNLIERFNEGKFDLILIKTTQQQVGSESIHVC
                     TEPVEWIGKKELLPLLDKPGPIPLVLSPTPCVYRGNVIDTLDKNHIDWRLVYSSASYA
                     GKMAAVRAGLGITAIQRSMIPNYLERLDYDFLPVLNDVHVSLLKKSESNKAIESLEFF
                     ILQKLKH"
     gene            28892..30412
                     /locus_tag="AC06IO_000702"
     CDS             28892..30412
                     /locus_tag="AC06IO_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HIO9873915.1"
                     /translation="MDKLLNGLSISLMLSPLIAMLFYSLLGKTRNIQATYYGTTVIGF
                     GFGLSLLALIVSANTVNQNSCFLFHASTLSLLLSTLILFISFIIHRFSLRYMHGDKCY
                     RRYFFKLSAITLTTVVMALADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTL
                     KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSPAIAVALGFIILTAMTQSAI
                     WPFHRWLMSSLNSPTPVSALMHAGLVNGGGFLIVKFAPLFVAKGNLLTILFIFGALTA
                     LLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHLCWHGLFKSYLFLNS
                     GSAVQQKKYQNKSRPEKLTTLLTAFFGGLGGMYGFALITGKPILSLHASTFLLGFAFI
                     TGTQLTLGLIAYEKHFKKVILAWALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSGL
                     QVLTLIIFFSLWLVFSMGLFKKIRETRLWDWFYMYMFNASTPHPKTITANRHTYYY"
     gene            30427..32715
                     /locus_tag="AC06IO_000703"
     CDS             30427..32715
                     /locus_tag="AC06IO_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HIO9873916.1"
                     /translation="MSSHITLIKTNTNNENKMTQFNYKVDNDVMIIRAMVNNVAKQMT
                     PVWPLEKFIACNALHGFESMSFEEAVIQNQTAKKGTPFNEKLERVNWHMIKWCGSFLD
                     IGQGTLEMPHRDKGLYFGFLKLAPFDSALHQNSKSIKSWLSNLPEMPEQAIRLCLDKL
                     GVLNQKQEDFLQSTLSHLPGWAGYIKWISEWKNRNGKEENPVSLVDFIAVRLVITCIL
                     WPEASQEEKKKKKDSADTKQLIQNIKNKEDDYRQLLLKKLLPELSKAHIKENRAHAQM
                     VFCIDVRSEPFRRCIEKLGHYETLGFAGFFGLPVSIKDYDGETIKDSCPVLLKPRFNI
                     HEKAIAANEHCIEHHEKGKEFKNILNRVYQQLKYNFSTPFALVESLGIWCGITMFLKS
                     CSPIFARRLTKDLNEMICPSIQTQPVFELDLLEKEVGISLQEQIAYAEMALRLMGLTD
                     NFAKLVIFCGHGSSTQNNPYASALDCGACGGNQGGKNAQLLASILNKIIVRRALAENG
                     INIPQDTLFYGAQHDTTTDEVEIYHSNVSQFIHQDILDQLRTDLNMAKYNNNLERINY
                     LNSIDCAEKDIARRSTDWSETRPEWGLARNAAFIVASRQLTKNINLEGRCFLHSYDWS
                     QDKDGTLLETILTAPMVVAQWINTQYLFSTIDNVAYGSGSKITHNVAGKIGVMQGNAS
                     DLMHGLPLQSVMSHDEQSFHEPQRLLTVVYAPREIISELVEKHDVLKTLFFNEWVHLV
                     AIDPRNHLFYKLEKTNTWSVIQ"
     gene            32729..33082
                     /locus_tag="AC06IO_000704"
     CDS             32729..33082
                     /locus_tag="AC06IO_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIO9873917.1"
                     /translation="MNIIKVSEKKEMNISLHPMKKIEVIVSGDNENMVATMMEKANIS
                     GFTLLRNISGKGHHGFHEGKILFNDKASFVMFIAVAPEEAIANIALGMKTLFQKNSGV
                     LFVSDVSVARVDYFS"
     gene            33107..33946
                     /locus_tag="AC06IO_000705"
     CDS             33107..33946
                     /locus_tag="AC06IO_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6671 family protein"
                     /protein_id="HIO9873918.1"
                     /translation="MHYQNQRVLLASKHQKEKAIQPPFEEKLGCRIHVPADYDTDQFG
                     TFTGEIPRKASAYETLILKAKAAAKQFGYRYVISSEGSFGPHPQLYFCPADTELMAFV
                     DLDNDLIIAEHEVSTETNYSHIDISATDDYEAFLNKAKFPSHGIIVRALNSEENYIQK
                     GICEREQLKTAIQKAFTYSTTIRLETDMRAMMNPLRMNVINKLAVKLVHRIQQYCPQC
                     HTPGFGKISSEGHLACISCGTPTELYLRKVLNCLKCEFKTYQARDDGLEFADQRYCPY
                     CNP"
     gene            34006..34506
                     /gene="purE"
                     /locus_tag="AC06IO_000706"
     CDS             34006..34506
                     /gene="purE"
                     /locus_tag="AC06IO_000706"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIO9873919.1"
                     /translation="MKPTLIGLIMGSQSDWQTLIHAAQTLDALNISYEAEIVSAHRTP
                     DKLFQYAEQAETRGLEVIIAGAGGAAHLPGMVAAKTSLPVLGVPVMSQTLNGVDSLLS
                     IVQMPAGIPVGTLSIGKAGAINSALFAAAILANKYPDIQAALKRYREQQTQKVLDNPN
                     PKEEKS"
     gene            34503..35582
                     /locus_tag="AC06IO_000707"
     CDS             34503..35582
                     /locus_tag="AC06IO_000707"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIO9873920.1"
                     /translation="MKLGILGGGQLARMLALAAYPLGINTVSLDPSPDACAGQLTRLI
                     VSDFNDELALKQFLSEVDCVTIETENIPFSCAELAAQIRPFYPSTQALKITQDRLYEK
                     NFFRTLNIPVPEFLPVESEDELMQAISVIGMPAVLKTRRFGYDGKGQYVLRTQADISK
                     SWGLLGSQALILEKLISFESEFSLIAVRNKRGETSFYPLIKNHHRKGILHFSEAPVHQ
                     DTLQQEAQTHAFKILEALNYVGVLTIEFFYQGNQLIANEIAPRVHNSGHWTIEGAHTS
                     QFENHLRALFDLPLGSTEVSSSCFLLNCIGNLLPLEPCLSIPGVHYHAYGKAPRPDRK
                     VGHVTLIDTHLERYAKSKDLLLGIS"
     gene            complement(35668..35880)
                     /gene="yacG"
                     /locus_tag="AC06IO_000708"
     CDS             complement(35668..35880)
                     /gene="yacG"
                     /locus_tag="AC06IO_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIO9873921.1"
                     /translation="MNNQQKIKCPICGKQNTWRPDNQFRPFCSERCKLIDLGEWASES
                     RKIPGSSIDPESIVASNNKQDNVDEQ"
     gene            complement(36002..36886)
                     /locus_tag="AC06IO_000709"
     CDS             complement(36002..36886)
                     /locus_tag="AC06IO_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIO9873922.1"
                     /translation="MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELG
                     ITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGS
                     STLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
                     SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVA
                     QSSQIDFMLELVAAGLGVTFLPRMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYL
                     SEAAQAWLALVREVHVDN"
     gene            36992..37381
                     /locus_tag="AC06IO_000710"
     CDS             36992..37381
                     /locus_tag="AC06IO_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIO9873923.1"
                     /translation="MANKIQQNSFFQIILVGLFWLTSELLLKLVKLPLSGGILGLGIV
                     LLLLATNCLKLNRIRRGAELLLANMLLFFIPGVLAILEHQEFIGLLGLKILFIILLST
                     ITVMLVTALVVDYCYRWRTHHAKQHSL"
     gene            37359..38066
                     /locus_tag="AC06IO_000711"
     CDS             37359..38066
                     /locus_tag="AC06IO_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIO9873924.1"
                     /translation="MPSSILSESMIQAFFWSLITIGMYFITQKLYRRWPFWWLMPLAL
                     TPILVASVVLLLHVNYQDYFRGTKWLVLLIGPATVAFAIPIYEKRALIRQHWPVLLIG
                     VIVGSFTAIVSSWGLAILLGLDDNLRLSLLPRSISTPFAIEISRGIGAAPDLTAIFVI
                     VTGILGAILGEFILSRLPFNSALARGALLGVGAHAAGTAQAHKIGNTEGAIASLAMIL
                     VGLFNILLLPLISHLLK"
     gene            38161..39576
                     /locus_tag="AC06IO_000712"
     CDS             38161..39576
                     /locus_tag="AC06IO_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIO9873925.1"
                     /translation="MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLG
                     QAQAWWLHHSLIALGKSLNQQGLSLVLRKGAPQEIILELVAQYGVESVYWNRCYEPQA
                     IARDKKIKLALGEREIKVQSFNGNLLHEPRAIKNKNGDFFKVFTPYWKQCRQILNIPS
                     PFMLTKRPRGIEITSDDLGKWKLLPAINWAARFSEYWIPGEEGAQKKLDEFIVHHLSG
                     YKRERDFPAKNATSRLSPHLHFGEISPWVILRALELAKLEQTCDLASVEHFLSELGWR
                     EFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGYPIIDAGMRELWATG
                     YMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPY
                     FRIFNPVLQSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDADASSIFLGKKYPKPII
                     DHQEARTKALSYYQQIKKGMD"
     gene            39609..40082
                     /locus_tag="AC06IO_000713"
     CDS             39609..40082
                     /locus_tag="AC06IO_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIO9873926.1"
                     /translation="MNKKSWTELIAWIITFEAIGFFLGMLTQANIPSWYEGLNKSGLT
                     PPGPVFSIVWSILYALLAIVGWVLWCSRNESEMRSVFYLYSVQMLMNWAWTPLFFVLH
                     WTGLGLLWILVMAGLTGLLILSLKNKKKSIAILLLPYLVWLLFAAYLNGVIWLLN"
     gene            complement(40224..>40819)
                     /gene="ravG"
                     /locus_tag="AC06IO_000714"
     CDS             complement(40224..>40819)
                     /gene="ravG"
                     /locus_tag="AC06IO_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavG"
                     /protein_id="HIO9873927.1"
                     /translation="CDMKNAAIIKNRIFLNLDKPVKRFLASDKADTPMTAEFYAEKDY
                     EQLFLDFLSQATGSYDEQISMLIAELDSGADRVAQKLMSALYSPWQKNLFPKAIKTIA
                     NKSEEYPLMSDLLIKFCQQHVGSVDAVDDFGESALAKILKKDQQRKSPLLFLVKHGAK
                     HCQLTSALQDSLIVNNSDIYNVAEDNTMDWISNCPQP"
BASE COUNT        12535 a         8302 c         7872 g        12110 t
ORIGIN      
        1 ttgatttata aacaaactgg tttggtcgct taactcgcca gaagttgaaa gtgctttaaa
       61 gatactcagt cgatgccctt tgtttacact catggacatg gaattgacat gagaagattt
      121 ctgtaacgta gtattgctca gattttcatc aataccctca agattaaaac cagaatttaa
      181 atcattagca aactgcaaat ttatcatatg cgcctctgca tccttcatca cttggctaaa
      241 cccctccgaa ctctcattac ctaatccttt acaagcttca gtaaaagatt gaatggtttt
      301 caaatcatta tttcctctta gaatggagct gaaatgcttg aagtcaatgg caagccgatg
      361 atttaaactg acaaaaacgt ccacttccaa ttgattgaga aacttataga aaggtttgcc
      421 ctctttttct acatcaaaga atttcttaat ttctccaagg aatggaataa aatctgaatt
      481 ccatatagca ctgtcattac ccgacttaat atagagtaag gtgagtccca aaatatgcct
      541 tgagaatttg accaaggtga ctgaaccgga tttttcattt tcgaattgta agaaagattt
      601 ctcaataagg cccaaaaaga attccatttc aggtttattg tctactcttt taacagccaa
      661 aaacagcttg tctaaaaaaa actcgccatc ttttctactt gcttccggaa gtttttcaag
      721 caagttgcgc attatatttt gataccacat atcattcacc tgtaattcat tagaacaaat
      781 gatatcaaaa actaattaag tgagtattaa gcaacctttc tcatttcaaa cttcctagct
      841 attataccga caattttttc aatccttcct caataatcga aaggccgatt tccaaatctt
      901 ttacgccagt agaaagtggc atgtggagac gaatgacgtt cccatatgtt ccacaggtca
      961 aaatgatcaa cccatgttct aaacaaaatt gagccaattg tactgcggct tccttattgg
     1021 gttctttcgt gtttttatcc tttaccaatt cgatcgcttg catgacgcct aaaccacgga
     1081 catcgcccac cactttgtat ttttccttaa attcagaaag tctggactgc agtgcctttg
     1141 caagatgggt aacgttttgc agcaggcttc cttcttcaaa aatatggaac acttcaagag
     1201 cagcagcaca actaagagga tttccaccaa aagttccacc caacccacct tccatagccg
     1261 catccatgat ttcagcttta cctgttacac cggcaagcac taccccaccg ccaaggcctt
     1321 ttgctgatat ggtgagatcg gggggtactc ccaacgtatt catcgcaaac agatttcctg
     1381 tgcggccaaa accggattgg atttcatcgg caataaaaac gatatcgttt gtcgtacaaa
     1441 actcacgaag tttttgcaaa aataaggcag gggattgaat aaatccacct tcacccaata
     1501 ccggctctat aatgactgca gcaatgtttt caacaccgac acggaaattg gcaagatcgg
     1561 taaactcatt gaaacattct tctacacaat tggctccctt ccaacgatac tcataaggaa
     1621 aaggagcacg atggatttca gaaggaaaag ggccgaatcc atgcttatag ggtttgtttt
     1681 tcgaagtgag tgtcattgcc atgtacgtgc gtccatgaaa agcatgatca aaacaaatca
     1741 ctgcttgttt gcccgtatag gcacgagcaa ttttaatggc attttcaact gcttccgcac
     1801 cagaattcaa aaggagcgat tttttttcga aatgtcccgg ggtgtgatca tttaattttt
     1861 cacacacctt gatataagat tcatagggaa taatattgaa gccggtgtgt ataaattttt
     1921 cggcctggag cttgatggca ttgaccaccg aatcaggaca gtgccctgta ttgaccaccc
     1981 caaaccctga tgaaaagtcc aaaaatacat ttccatcaac atcctccaca aaagaacctt
     2041 tggcctgctt cacaaagata ggagtagcat gaaatggccc tcttgcaaca tgctgacgtc
     2101 gcaattccat caactgttgc gatttgggtc cgggtattgg cgttttgata taaatttttt
     2161 tcattagttc agtaccaacc aattggtgtt tcatccaggt taatattcac ttgcttgacc
     2221 tcaaggtaac tctcaatgcc atacaagcct aactcacgac ccgctccact ttgtttgtat
     2281 ccgccccaag gcatttcatt ataggttgga tggtaatgat tcacccaaag aatcccggca
     2341 cggatttgag atgtgactcg atgggcccga gtaacgctct tcgtccatac agccccagat
     2401 aaaccataat cagtatcgtt cgcaatgcga atggcttctt cttctgtatc aaaggggatg
     2461 accacaagta ctggaccaaa aatttcttca cgagcaatgc gcatcgtgga tttcacttca
     2521 tcaaaaatgg ttggctcgaa aaaatgtccc tttgcaaatt cactgcctgt cgggcgttta
     2581 ccgccgcaaa gtaatttggc tccttcttcc attccaagct tgacatagct ttccactttt
     2641 tcacgatgtg cacttgaaac caaaggcccc atttttaccc cggcgtccaa accatggcca
     2701 agcttgatat ttggaatttt tttcaacatt ccctctacta tttttttgtg aatagggcgc
     2761 tctactagca agcgggaacc cgcagaacag acttcccctt gatttgcaaa agcaccaaaa
     2821 agggcaccgt cgattgccat ttcaaggtca cagtctgcaa agacaatatt tggatttttt
     2881 ccgccaagct ctaacgagat ttttttcaaa ttgccagtag ctgcctgcat gatttttctg
     2941 cctgttgccg atcctccggt gaaagcaacc ttatctacca tagcattact tgccaactcc
     3001 tctccaactc ctatacccgg cccagtaatg aggttaacca cgcccgctgg aaatccgcat
     3061 tgatcaatca acttgaataa ctcaagtgcc gttatcgggg tcaattctga tggttttaat
     3121 accacggtat tgcctgcggc aagtgcggga gcaagcttcc aggccgccat aagcagtgga
     3181 aaattccatg gaatgatttg cccacacacg cctatgggtt cacgtactac gtaactaaat
     3241 gaattcgctg gaaccgacat cgtttcacca tgaattttag tcgcaagccc accgtaaaac
     3301 tcaaagcaat tggcagcatc agtgacatca aactccgctt cgggtaacgg ttttccgcaa
     3361 tttctagttt ccagctctgc caacatttta gcattggcac gaatcaaatc agcaagcttg
     3421 aataacaatt tgcctcgatc aagtgccagt gattttctcc actctccttc atcgaaggct
     3481 tttctcgctg atttgatagc tagtatggca tcttctttgg cactttctgg cactcttgca
     3541 atcaattccc cgctagccgg atcaaaaatg tcgcgagtag ttccactttt ggctaaagta
     3601 aattttccat caatgtacat ttcgtaaatt tccatttatt cagcaccttt tttaggaaga
     3661 ggttaaaaat tttagtatgg catcattgac taacgcgggg tgttccaagg gactggaatg
     3721 tccaccagga attgtaatga attgagcatt agggatccct tgtgccagtt gttgactttc
     3781 tgcaggcaag gtcagtatat cgtcctctgc tgctataact agtgtgggca ctttaatttc
     3841 atgtagccac ccgcgggaat caaatggtgg tatgcttcta aactgtcgcg cttgatcttg
     3901 taaggattgc ggataaggat tatttttcaa attctcttta aaagtagcga tatttttagg
     3961 ctcagccaaa tattcgcttg aaaaaaacca tggtattcca gcctctatta ataaatcgaa
     4021 tgaaatattc tcctttcgca aattcaaaag gctttccata gcctggtttg ctctcgtatt
     4081 gaattttgca accgaattta aaataactaa tttactgatt tttttaccat gctttcttgc
     4141 aagcagttga gcaatagcac cacccattga ttgccccaga atagaggggt ttctaaaacc
     4201 taattgctcc aagaaagcca tgatgtcatc cgcctgagct tctaatgtaa aagaacctcc
     4261 atcgtctctt gtttgcccta tcccgcgatt gtcaaatatc aatatctgga attctttagc
     4321 caactcatgg agcatggcgt tccaaaaggt gtgatcacag caatagcctg caattaaaac
     4381 cagtggttta ccttgcccat agagctcata ataaatatca acaccatgaa tcttgagtgt
     4441 ggccatgtcg caaaaactcc ttaattgatt tttttgacga acaaacgcaa cgcttcattc
     4501 aatgactttt cgcctcaatt atctattgtt tcgatgaatt aagtcatttg tcaacaaacg
     4561 tgtttgataa gctcctggcg ccataattgt tagtttttat aaaatatgac tgctcaatta
     4621 ccatcaagat aattgatgca gaaatcaatt cctcttaata atttcttaat taaggtttaa
     4681 tatactcatc gcttcatcgc cttcctatct attttagatg aaaataaatg cgttatgtta
     4741 attttgaatt attaaagaga atccctcagc atcttaaagg ggttatggaa tactacccgg
     4801 atgtgcttct gtttcaattg aatcaaatac agtataccca tttatggcat gagttatctt
     4861 ccgcaaaata cctgtatact aacggatttg tgataagacc tacccattgg ttaatgtatg
     4921 cctttcaaac ggttaaaggc tggcttggct ttgataatca ttgtcagccg gagaaaattt
     4981 catacgtttt agacaaactc tcgtattatg gatacacaaa acaatttctt cagcctgatt
     5041 tttcatcaat aacaaatttc tcaatctctc ctgaaatttc tgccctggtt gtcaaaagca
     5101 gagatgattt aacgacagcc caactacaaa ataaattggt gaataattat tttaaagtgg
     5161 agccccatct tggaattgat tattcctacc agaaactaaa cccaaatcat cgctttggtg
     5221 aaagctggat gcacgcacac gagtggggtc tgattccgca attagaccct caagatgaca
     5281 gtttgatcgc ggaggtaatc agcaccctgg ataagaaggg gatttcagca aaagatgtgt
     5341 tctttttgca acatagcaaa tacgccaaag ctgctgctca gtactattgt gataaggcta
     5401 aaaatacaat tgtcccttcc tttttcttcc gcttgctttg gacagacccg cgccctcgct
     5461 atctcgcttt ggcccttgct tacgatcctg aaatagccaa gcgagatgcc cagaaattca
     5521 ttgaatatca tcttgcacaa aaggaatatg acaatgcatt taatctattg ggcatattga
     5581 acgactccaa tctggtttta aaatttttgt tagcgattcc agaaacagaa cgtcatgctt
     5641 taattcaaaa agacacagca atcgccgcca tcatggcgaa atattatatt gaaaaaaaac
     5701 aatattttct tgcaacacaa ttttacacca atattgaaga aattagccct aatgcagcat
     5761 ttgctattga aattcaagag caaaattatg aaaaggctta tgatatcttt aagaaataca
     5821 actcccctga cctattttca atgcctgagc gtaaattgct tgccaaggta ttttatagtg
     5881 aagctgaaac agcctacgtg gccgggaaaa catatagagg aaataaaagt tgggaaaaag
     5941 caaaacaata ttatctgcaa agcctggagc aaaaaaaggc agcgcaccac ctggatcctt
     6001 ctgacgaata cctggaagat ttatacgctc acaaacgctt atatgccttg ttattaatcg
     6061 acgcggatat cgacctgcac aaagcagagg acagtgatat tgccagcatc caaaaagcaa
     6121 tcactctgtt gagagaatgc cgctcaaaca ataaagaaga acaagaacat cataagcgcg
     6181 ccctggcagc cggacttatg cgccgagtag atactttgcg tgaaaaaatt gcttttaatt
     6241 attactcttc tgattttgag tctattagaa aacataaaat cgaacaccaa caagatattg
     6301 cgattttaat taaaacattg ggggagttaa ttgctctatt ggaaggaact caagacaaag
     6361 ccttgcgctt gcaactgggt aaagcacatt atctcctggc cgatgttcaa tgcttttttg
     6421 atatcaatgc cccagacatt aaccagcatt ataaaatggc gatgaaagca gtaccaggaa
     6481 acccctttta tgtgctacgc gtagcagaat tatttgaaga ggaaaaaagc aaactgcaaa
     6541 aaatcggcat aacacagctg aaaaatatgg ggtaccaggt cttcgatttt cttcattggt
     6601 ctgaagagcg ttggtgtaaa cgagacgata tcattcataa tatcaaagat atccacatgc
     6661 cgccctcaga accaattaat agctcatcat ggaattttac gttttaatta atagctacaa
     6721 agtataaaat aacctcggtt tgcgggcttt ctttaaaata tacttttcca ttactgccaa
     6781 ttgaaggagg catttagaat aatggcaact tattatggtc aatgcgcttg tggaaaaatt
     6841 caatttatgt gtacaggtga gcctgtcttt actcaatatt gtcattgcaa taaatgtcgg
     6901 gaaattgctt ctttatctca aaagaaacag gataaatcag gttactcgct cactgcagcc
     6961 tatttgacca gagacttcag gattgtgtct gaggagaatg attttgaaga aatcattaaa
     7021 gaaaaggcaa aattatttct ttgctcatat tgtcacagcc ttgtttatgg tattgcactt
     7081 gatcccgcgc aacaggatag tattgggatt aatgtaaata actttagctt tgataagagc
     7141 atcccagatt catttaaacc agtaaggcac atatggtatg ccagtcgtat tatggattgc
     7201 gatgatcaac tgcctaaatt taaagacgct cccaaagagc agtttggttc aggggaatta
     7261 tttgaattgc ctaatgccaa ctgataaacc gtattctagg cctttatctt tccaaaatat
     7321 ccagcacact tcattgatct ggctctcata ttttttcaat ccgacaaaac accgttttgc
     7381 caaagctaat tcaaacagga tttacgaaaa accccaattt ctatgttgaa aaatgttttt
     7441 aattcggtca tttagtttat ctaaactccc tcactaaaaa cattttttgc cttgagcttg
     7501 gggtttttca taagtcctgt caaataattt ttgtctttta tatccacgat aaaaaccaaa
     7561 agacattggg atcaaatctt aagccggatg ttttttttat ctcttaatat tctcttaaag
     7621 tttgacagct ataatattta tccactgtgc taatttttaa gtcacgaata ttaattttaa
     7681 agtatttatc caggagttgt gtaatgccta agtacgttga agggatagaa ttaacccaag
     7741 aagggatgca tgctattttt gaacgcatgg gacatccaaa tattaccagc ggtactatct
     7801 acaacggaga gcctacaatt gataaaggtg ctcttgacag gcaaggcttt atgccagtat
     7861 tgaccggggt cagcccaagg caagactcgg gacattggat tatgttaatt aaaggtcaag
     7921 gcaatcaata tttcctcttt gacccgctgg gagaaagctc gggaaaatat taccaaaata
     7981 ttttagctaa aaaactacct ggcgctacac tttctgtaat tcctaataat gctggattaa
     8041 atatgggatt atgcggctat tgggttgcct cagttggttt aagagctcat gcggcattaa
     8101 cacaacctat tcccccttct ttaagaaatg tgggtcaaac cattacccag gaaatgcgag
     8161 atgaactaac acaagatgga agcgaaaaaa ttacacgatg gttacgggcc gttggcaatg
     8221 aatttccaga tggtgatata caacctgatg ccacagcctt aagacgtgct actgaaaaga
     8281 atgtacgtat tgatgagttt cagcctgttt taactggaac atcacctaaa gaaatttcga
     8341 taaatcctac tgctcctcaa gaagtatccg ttcccacgtg gaatggtttt tccttatata
     8401 ctgatgaaac cgtgagaaac gcagccagat acgcgtatga taattacttg ggaaaacctt
     8461 ataccggtac agtagaagcg acacctgtca attttggtgg gcaaatggta tacaggcaac
     8521 accatggtct tgcccacaca ttgcgcacca tggcctatgc ggaaatcatt gttgaggaag
     8581 cgcgaaaagc aaaattaagg ggtgaatcat taaaaacatt tgctgatggt cgtactctgg
     8641 cggatgttac gcctgaagaa ttgagaaaaa tcatgatcgc ccaggccttc tttgtaactg
     8701 gaagagacga tgaagaatcc tccaagaatt atgaaaaata ccacgaacaa agccgagatg
     8761 cttttttgaa atatgtcgaa gagaataaat ccactttaat tcccgatgta ttcaaagatg
     8821 aaaaagatgt caaattctat gctgatgtca ttgaagataa agatcataaa tgggcagatt
     8881 caccggcgca tgtcctggtc aatcagggcc atatggttga tttggtcaga gtaaaacaac
     8941 cccctgaatc ttacctggaa tactattttt cacaacttca accctggata ggttcaacag
     9001 ctacggaagc agtttttgcg acacagagac aatttttcca tgccacctat gaagcagttg
     9061 cgggctttga tagcgaaaac aaagaacctc atctggtggt cgatggttta ggtcgctatg
     9121 tgattggaca agatggcaat ccaatacgag aagaatctga tgacgaagac gaggaagaat
     9181 caggtgaatt aaaatttttc tcacaaaaaa agaaactcga ggaaaaccaa cgttacatgc
     9241 gcgttgatga atatttaaaa cttgatgagg tccaaaagcg attccctggc gctggtaaaa
     9301 aactcgatgg ggggctgcct ggactgaaag aatatcaata tttacaacgc ttgaactcaa
     9361 taaacagagc acggtgtgaa aacgacgtcg acttttgttt gggacaatta caaacggcac
     9421 atcatcaaac caaaattact ccaataaaaa gagccttcca atccagctct gataaagtga
     9481 gaagacaacc caatatggat gaaattgctg ctgcccgtat tattcagcaa atcatggcca
     9541 atcctgattg catccatgac gatcatgtgc tgctcaatgg tcaaaaactg gaagaaaaat
     9601 ttttccgtaa tttgttggcg aaatgtgaca tggctattgt agggtcgcta ttaaacgata
     9661 cagacattag aaatatcgac accttaatgc aacatgagag aaacacagaa tttcactcaa
     9721 cagatgccaa agcaaaaccg gtgaaactag gagaaacatg ggaaaaaaca atcagatcgg
     9781 gtggtggggt tactcagata aaacatgatt taattttcct catgcaaaat gatgcctggt
     9841 accatactcg agtcaatgcg attgcacaaa accgggataa agattcaaca ttcaaagaag
     9901 tgttgattac cgctttaatg acaccattga ctaacaaatc attgatagat acatcacgtt
     9961 ccccggcccc aaaaacgcta ttccgtggct tggatctatc agaagagttt aaaaataaat
    10021 taattaacca ggctgaaacc atcattgcca atacgacaga acatctcttt actgatctgt
    10081 cgacagaagc ctttaaacaa atcaaattaa atgactttag ccaggtttct gctagaactt
    10141 gtgccagtac ttccactaac attgaagttc ccaggacaat ctttggctcc aataccatct
    10201 ttgaaatact tgatcccgat ggtttattac accccaaaca agttggaacc catgtatcag
    10261 ggtcagaaag tgaatattcc atttatttgc cggaagatgt tgccttggtt ccaatcaaag
    10321 taagctttga tggaaaaaca ggtaaaggca aagaccgaca tatttttact ttagtcgcgg
    10381 taaaaagccc tgatttcacg ccaaagcatg aaagtggtta tgctgttgaa cctcttttga
    10441 aaatgcaaac gccaaaatta gaagaaattc aaagattagt tgagcaagca agggaagaac
    10501 ctgacctgga aagggtattc aatttacaat cgagagtagc aagacaagcc agattacact
    10561 ccactgaatc cggatataaa acctttctga atgaaagagt tgcgccggta ttggagcaaa
    10621 gcctcaatgg cctcttggac aataatgcag tcactctagg caaagtatta tcagcattcc
    10681 cttcagacgg gcaatggtct gcctttaatt cagttgaagc aaggcaaatg aaaatacaga
    10741 tggatgccat taagcaaatg gttgaaaaga aagccgtcct cgaaggtcaa attctccctg
    10801 ccttggctca atgccaaaat gctttggaga aacaaaatat tgcaggtgca ttacaagcac
    10861 ttagaaacat cccttctgaa aaagaaatgc aaaccatgct gtcaattagt ggtggtttga
    10921 gaggacaaat ccagcgtacc aagcaagatt tgactgaaac tctggaacca cttcaacgcg
    10981 ccataactgc taaattggta agcgaacaag aaaaagtgaa agtgcgttat gaaaagctga
    11041 tagcaagcat acctcaacgg atcgccgatc ttgaaaaagc tgcgctttct gatcttgccg
    11101 gtgttaaaaa agttgtctct cattttaatc agctgcaaga agaattaaag ttgctgcgta
    11161 atgaaaaaat tcgcatgcac actggctctg aaaaaattga tttttcagat atcgcacaat
    11221 tagaggcgca actacaaaaa atccatacta aattatatga cgcctatttg gttgaattaa
    11281 ctaaagaaat ttcagcactg gttaaagaaa aaccgaaaaa cctggccgac gtgaaaagga
    11341 tggtttcgaa tttctatgct atgtcagctg atattgaaca attgcgtcag gaaaagataa
    11401 aagaacacag tgagtcaaaa gatccgattg atatgtctga tattgataaa ttaaaggaag
    11461 aactacaaaa aattaatcaa tttttagtga aggcgatggg aactaatatc agggtttcac
    11521 tgaatcaaat ggaagtaaaa acttttgatg cccaggaaaa agaagcacaa caaaatttga
    11581 agcaactaga cgcactgatt aataaattgg aaagttctca tgctgtacag aaacaaaaag
    11641 aagaactcga aaaattaaac caattgcttg tcgagaagcg aaaagcttat cccgcaatgg
    11701 tgcagttgca attcaggagt gaagcattaa ttatccattt acgtgaatta tgtgaagctc
    11761 atcaagctca aatggctaaa acaagaaatg taagagcgca agaaatcacc aatggacgat
    11821 ggaaagtgca atggttgact gattgggttg gtttaactac agatgagcgg gtaactcttg
    11881 ccaacaaaga gaaagaattg gctaaattca aggaagattt aaataatgac gagtatgatt
    11941 tgcaggaact cataagtaac ctggctgaga aaaacccgtc agaattggaa gaagccatag
    12001 ggatatctaa agagagcgct cagaaattgc ataaactgtt aactcatcta aaccacagta
    12061 caacatttat gaataaaatc gaccaacgat tacagtctat tgatgagcta ttaaatgagt
    12121 ttggcaagca atcgccccga actgaaatga taaaaactgt tgaggaaaaa caaggaactc
    12181 tcttaagatt gtaattcttt atcttccacg gtagcccatt aagggctacc ccgtgggata
    12241 ttttagaaga ttacggaaaa taagattata attatcacct cttgccagat ttaaaatgca
    12301 aggatatatc taatcgctgc catctaattt atttttgctc gcaatacccc atcacaacac
    12361 ttaccacagc attagctcct aaaaaccgta tttttgccct attttttatg agtgagcgaa
    12421 attgaacaaa tagctctctt gtgctaggta tataaatatg gaaagcgtta ctatttctaa
    12481 ggatgcttat ctgtgaagaa aacaggaagt gatgttctca aagaatttat aacgaccttt
    12541 gaaattaatt atatttttgg taaccccggc actacagaat tgaaatttct ggaagcgatt
    12601 gagcagtgtg ataatgccac ctattttctc acactacaag aatccagtac cgtaggtata
    12661 gccgcaggct atgccatgat caccaggaaa ccagctctta ttaatttaca taattatgtt
    12721 ggattagcga atgctgtctg taatatgtat aatgcgttca cttcaggaat tcccctgcta
    12781 ataattgctg gccaacattc acgtccttat ttaattcata gcccagtcat gtccggagac
    12841 ttgacctctt tagctcacac tgcagtaaaa tatgcctatg aggtgaattc cgccagcgat
    12901 ctggccgttg ctttacagcg tggttatgct caggcaattt tgcctgggcc aggtccggtt
    12961 tttctatcaa tccctatgga catttggcaa gaagaaactc aagaaaccac gataaagaag
    13021 acaagatttt tacatgcttg catcaacgct gaaatagagc aaatttgtga ggttttaaca
    13081 aataccccca aaggaaaatt ggcatttata gcggattatg aggtaggtgc atcgaatagt
    13141 attcgccttt tacataccat tgccgatcag ctaaatgccg atatttatgc tgccccctat
    13201 catgtgcgtg aagtaattaa tcccttaagt gtaaattaca aaggtagatt gtcagataaa
    13261 tctggagaag tgcacaacct gctgctgcaa tatgaaactg tggtactttt aggcgaaaaa
    13321 gtgaaagggt atttgtatac tggtcaatct tctattccac agggtatacg attcatacaa
    13381 atcagtgccg catcggagca tctggccttt gactatcctt gcgatctggc aattcttggc
    13441 aatcttgagg cgaccttaac tgctattagc aagcatttaa aaattaatgg gctaaatgtg
    13501 ccgcttaaat cagccaccaa cattaaaaat ttggaaaaaa aatataagca acatcctatg
    13561 cattcgttga ttgttaccct cctgaatcaa gtaaatcgct ctgttcattt aataacagaa
    13621 ggatcatcag aagatgctat tgtgcaggac attgcaacaa cactaggttt tttaaatgtg
    13681 aatttttcac caagaggggg tggacttggc tgggctatgc cattagcaat aggtatttct
    13741 ctagcgacca gacaccattc aatttgtttt gtaggagatg ggggcagcat gtatgcgatt
    13801 cattcaatat ggtcagctgc caaatataag attcctgtca ttttcatttg ttttgttaat
    13861 cgagaataca gagtattaaa aacagatatg tctcaaaata gatctttcac agaagaagat
    13921 ttttttcgcc tggatttgaa tcatccacca attgatgttc aacaaattgc cttatctttc
    13981 ggggcacagg tgaagaccat taaatcaaaa gaagaagtag agactgttct caagagtagc
    14041 tttgcattcc aaggcccaac atttattacc ctatatcatc aataaaacat ttcgaaagcc
    14101 agacatcggc ttttgcatta tctaaaaatg taaagcttaa aatcagtctc attatttaaa
    14161 aatttttatt ttacaactca taggaaaatt attgccggga attaattttt caggttagtc
    14221 agattgcaat cagaacacaa gcctttgatt tctaccgtac aattaataat tgaaaattga
    14281 atggaattcg ccgcttctgt taccgcagta aaatcaacta cacattccaa ttctttgaca
    14341 aaatcacact gattgcatat aagaaattgg gcttgtccca catgatgccc atgcaaacag
    14401 gcgacatagg atttcagact gtcaatgcaa tgaataaatc cctcttgatg ccaaaattga
    14461 atagcccggt agaccgttgg tggcttgggg ttatccactt ccatagataa tctttgcagt
    14521 atgtcataag ccccgagtgg ctttctctca tcgactaata ttttgaggac acgctccctt
    14581 ggctcggtaa atcgatagcc atggtttaca caatgtttat aagccttatc caacaaatta
    14641 tgtttcatta atcttcttac aagcgaatag ttagtgcgca ctattttatc ataatccctg
    14701 ttgcaaggaa aaatgatcaa acaacagcca atcattattt catagaaatc atttactgtc
    14761 taaaaattat ttaatttttc ttgttatcta acaattaaat gttacaatat aacatttttt
    14821 gagaaaggca gtatgtgctt gtaacgcctg gcttaggaag aaatcttatt agggacaaac
    14881 cgctggctct attgtgcaga cccagtttca aaataggcat ttgcagaaaa tcaacctttc
    14941 tctatagcaa ccagaacccc tttaaataga ggaaagccat gctgtatagt aattatcgat
    15001 tctaccttgc aaaagatgcc caacttctca acccggaaga atccagagaa gcaattgagc
    15061 agattttagc taaaactaaa gagttggagt taataagagt agctgtcgat ttgactagtt
    15121 caaataaaag catcagctta ttgagcgcat ccaaagagaa tcatgtcata aaaaccaaag
    15181 tgacccagat tttaaaggag cagggccttt ctgtaataga catgatggaa gtggaagtgc
    15241 tccaggatat tgaaaaaata gagaatcgcc atcctactca taagtctaag aacacacatc
    15301 ttcatcccat taaaaagcac caaaaaaaag caaaacatga ccattctcat gggcatgggc
    15361 accaccatgg acatcatcat gaaaatcatt ggatcaaagc agcattaggt ttgatttggg
    15421 gaattggatt acttgttctt tctattggga cttttaatat tcccctggtt gcttactatg
    15481 tcattacagg actgacctct ttgatgactt tgtatttggg tcatacggtt tatcaatcag
    15541 cctggcaagc attgaaagaa aaaaaatggg acaccactac tttatactca attagcactc
    15601 taactattct tgctgtttct attgcgagcg tttttgtccc tggcctaccc atgatgtttg
    15661 aggcagcccc tctggtttta ggtttttggc atttaggtga aggaattgaa catactctgg
    15721 tcggcaaaat caataaaaaa ttggatgtcc gggattgtgt accaccatta gtcctgttaa
    15781 aaggtaatcc ggataaagag atttcagtca agacattaat tcctaacgac atgattattg
    15841 ttaaaaaagg acaggtcatt cctgttgatg gtgtcttaac gcaaaaagcg ctcctttata
    15901 ccacaagaat tgatggctcg cccaaactca aagaatttca ccctggcgat gttgtaaaag
    15961 ccggcatgca attggccgat catattcctt ctctggaaat gcaggttacc aaaacttatc
    16021 aaaattctta tttgtcattg attgccgaaa acattaataa agcgaatgat gagaaagcgc
    16081 ctatcgaaat gtttgcaaac aaggtattga aatattttat tcctggtttg attgcggtag
    16141 cgctcctatc ggggattgtc atcggttcgt tgtttaaccc ggcactggct attcaatgcg
    16201 tcgtgtctgt tctggtaagt gcctgcccgt gcgctttgag tttaattacc ccaatggcag
    16261 ttaaaatcgg tatgaaaaaa gcctctgaaa acggcatcca ctttaacaac ggtaaagcct
    16321 tgcaagcagc agccgacatc gataccgttg tttttgattt aaatggcact ctgactcagg
    16381 gcaaaatgtt ggtgaaatca ttgcatatta ccgataaaaa gtatttgcgc cctattgcat
    16441 tactagaaag ccagtcggat catccggtag cgaaaattat taaatcttac atcgaacagc
    16501 aagacacggc ctcaaccgaa ccattagaaa taacgtcaat tgataagagt catcattccg
    16561 gcatgaaagg tattattaat ggcgagacct ttatgatagg gaataaagac atgcttgcag
    16621 cgaatggaat tacctctatc gacaaaccct atgataatcc tgaaaaaggt tctgtttata
    16681 ttgtgcgtgg gacaacggtg ataggtcaaa ttgccttaat tgatccgttg cgagaggacg
    16741 ccattgccac agtgaaacaa ttgaaacgtc ttggtaaatc cgtacatatt tgcaccgggg
    16801 cagatcaggc tactgcagaa aaatatgcgg cattattagg cattcccaaa gataatatct
    16861 gtgcgaacac cgtgggggct gtcacgcaaa agggggaagt atccaaaacc agttatattc
    16921 aacaattaaa gcaaaaaggc tttaaagtag ctatggttgg tgatgccgct aatgacgcta
    16981 ccgcaattgc ctattccgat ataggcattg ctgttaaatc aagtatcggc gactccataa
    17041 cagaacaaca tgccggcata gtcgtacaac aaggtttatt atttcctata gcaacagcat
    17101 ttgatgtagc tcaaaaaacc aaacaaaata ttttccaaaa cctgttcgtg agcctaagct
    17161 ataactcaat aattacgctt gtagcagcgg ggttgtttgt ggcattaggt tttgcactaa
    17221 atcctgtgct gggtgtcgca ttgatggtgc ttgaatccac tattgttttg acgaacttat
    17281 accttttcaa gcaacaagac attgtgtcct caacatcatc caatgccaat aatgctgtag
    17341 aggtcgagcc cactgccaat acaacatcaa aagtcttgca tgctttaggg catcgttccc
    17401 agcctcaaga aaatttagca attgtgacag agtctaaacc agtcaatccc aaagtaataa
    17461 atttgtttgc gcctaaagaa catccttctt ataggtctga tgtcactcaa caaaaattgg
    17521 ggtgtggaat ctgttgatca agcggtactg aaattgcctt gattttctac ttcaaggcag
    17581 ttgttactcc tgttgcctca taaataactg ttttttggac tatactctta tcaagaccat
    17641 gcaaagcatc aagccataaa attaggatga ttcaatgcca tgaatagcac ttggcatcaa
    17701 accgccgctg gaaaccgctc cattttaagt aatttgcgat taagcagaac tctacttaac
    17761 gtcatcttta ttattgccct ttccctgctt ttaatcatca atattatttc ttataaccag
    17821 gtcaaaaatt taatcttcgc cagtaactgg gtttctcata ctcatgaggt catccaaacc
    17881 atagatacat gcctttatga tgtagtagaa attgaatctc gccagagatt ttacttgatt
    17941 cgaggttcaa gccctcagtt tatgactgat gtagatgata tgaaatcaac attacaactg
    18001 aatttagaca aacttgagca attaaccagg gacaacccag aacaaaacaa acgaacccat
    18061 cgttttattg atttggttaa acaaagatta aatttgttaa gccaacttgt acagttaaag
    18121 ggaagcggca aactcgatac acaagaaggt tatgatttac ttaaccaaag tcaagacatg
    18181 tcgcatcaag ttaaaagcct tggacaagaa attaaatctg ttgaattggt gttgctatca
    18241 gaaagaaata ctggtgcggt tcgctgggca gataccagta gttttatttt aatatccgga
    18301 ggtattacaa gcatcctgtt tttaacgatc gcttttattc tggcaaatat tgaattgtca
    18361 acacgcagaa aaactgaact acagaatcaa aatacgcaaa tacgattgaa gaaaattata
    18421 gaaagtgcca gtgatatgat agcagcgttt gacaaagacc agcgatttat cacttttaat
    18481 gaagcttatc agcgtgagtt taaacgtctt tttgataaat ccataagcat caatatgtct
    18541 ctggaagaag cgttcgatga tgttcctgaa aacaaaaaaa aattggtgca aacctggaaa
    18601 gaatctctac aaagggatga agaaactaaa aatatcgaag tgaatacaga acaggaaaag
    18661 actatttatg aaatgacctc caaactcatc caaaacggtg acaatgaaat taaaggtgtg
    18721 gtacacagcg ttcgcaatat taccaaacgg gtacaagaac ataccgaact acaagaatcg
    18781 tatgaaaaac tggctaatgg aatgaaaaaa ctgcaagaga aaaatgaaca aattaccctg
    18841 ctggttgaga tgagtgacat catgctggct tgtggctccc aggaagagct aagcgacgta
    18901 atggcgaaat actcccaacg actactgcaa ttttcaagcg gctacctgtt tatcatgcat
    18961 ccttccaaaa attatttgga aaaagccgcc agttggggta atcctcaacc gcacgatctg
    19021 acttttaccc cggagcaatg ttgggctatc cggcttggtc gaattcatta tgcaggttca
    19081 tcgcgtatcg agttgatgtg tagtcatacg atgtttgccg agcaacccga gttgtctctg
    19141 ctttgtgtac ccctaatggc ccaaaacgac atttacggct tgttatacct tgaagttggc
    19201 ctaaaatttg atgaaaacca acaattacta ataaccgcat ttgcagagct taccgccttg
    19261 gctttggcaa atgtccgcct gagagaaaat ttacgctatc aatctatccg tgatccactc
    19321 actggattat acaatcgccg ttatctggaa gattttctat ttaaacaatt acatcaggct
    19381 gaacgaacaa aagcctcatt tgccatttta atgctggatt tggatcattt taaaaagatc
    19441 aacgatacct ttggtcatga cgcaggagat cttgtattaa aagagctggg gcaaattttg
    19501 aacagtgata ttcgcctcgg tgatattgca gcccgatatg ggggagaaga atttgtcctg
    19561 ctgctctatg atattgatgc tcaagccgct aaaatgaaag ccgaaaattt gcgttcggcc
    19621 atttccaatt tacaggttaa atacggagcg caacctgttg ggcagataac ggcctcaatc
    19681 ggaatatccg tctatcctga cgatgccaaa tcacctgctg aagtgattga agcagctgat
    19741 aaggctttat accaagccaa aaacaaaggc aggaataagg taattttatt ctcggaaata
    19801 tcatcctgat ttggattgca gctcctgacc taaatgttgt gatgtttctt atgtccgata
    19861 gagaggttca tgctttatat gagttttgag agcgaaacaa tagatactac actctatagc
    19921 atccggtatt tttagtagca accccatcta cgataatata taatgcgcag ccatcaggca
    19981 taatctcttc tattcatgct ctatcaagga attatctcga atgtttagca aagccctaat
    20041 cagtgcaacc tataaaaatg gaaaacccgg ccaacttaca gaagaacatg aaaaagcaga
    20101 gcatcaccgc tttaagacag actatctgtt taaaaatgca gagatctcta aagacttata
    20161 tgcttcacgc ttgattcagc attttttgat tattaaagcc atagaaacaa aattacaaaa
    20221 tttaccaaaa acagaacagt cagaaatcag cgcatttttt gctttatctt atcttgagca
    20281 cttatggcgt actccagcta tagaaaatga tttacgacaa ctaaacatta atcctgatga
    20341 aattcataag gatgtgataa caaaaacaac tgaaaattac cttgaaaaca tagaaaaatt
    20401 tacacccaaa atccttctcg ctcatttttt attacatatt gccgggttta tgcatggcgg
    20461 gaatattatc cgctcgaaat acattgaacc aagcaatcgc ttgaccacct accaaataac
    20521 ggcagatcaa tatgattttt ctgctgccat ctccttttta tcctcgggaa aacattcacc
    20581 gctcgctctt tatcaggata tgatgaaaca aatcgataac attgttctaa ctggtgatga
    20641 gtatcaagag cttcttgaac aatgcaaagg catctatgaa accatgagta atatctacga
    20701 tgacttatgt gatatgcata ctcatcacct gaagctgccc acctactcac ttgcagctat
    20761 cagcgtcagt ctgattgcac ttggattaat tctaaaacta ctgatagatt gcttaaatcc
    20821 aatgacgaat cctctgtcaa atgctcctcg ttaagatcca tcggacagat aacgttgttt
    20881 tgataaacaa atggaacagt aacagatgtt aaaacgggat atttcaaccc tgaatatatt
    20941 agttgcatca gctggtggaa tgattggttc aggttggttg ttcagcccat ttattagtgc
    21001 ccaaatggca ggaagtaatt ctctgataag ctgggttatt gcagcaatat ttatgttgtt
    21061 tattgccctt ccgctttgtg aactgggaac gatgtttcca gtttccgggg gcatgtctaa
    21121 ttatcctact tacacacacg gtcgagaagt ggggttttta ttcgcatgga catcctggtt
    21181 atcttatgtt gttatgacgc ctattgagat acaagcaatt ttgcaatatt caagccattt
    21241 ttttccaata ttaatcgaca atcatagcga atcactcaca ctgtccaatt atggttatct
    21301 tgctgcaatt gggattatgt ttttcgtagt tattttgaat tcttatggga ttaagttttt
    21361 ggccgagtgt aacaagtatg ccagcgtcat taaatttgtt ttgccgagta tcgctattat
    21421 ttcactgtta aagcaatcac cctctcttgc taatgttaat attaatctgt caagcaaaga
    21481 acactgggta gagatattta ccgctctttc tgcaggcggt gtagcttttg cttttacagg
    21541 gtttcaaaat ggtttaatcc tggcaggaga agttaaaaat ccacaacgaa atattcctat
    21601 tgccatactt ggcgcggtat tggttggctt tattctttat tttatgttgc agttcagttt
    21661 tatagcggcg gtaccacaaa agtatttggc tcaaggttgg cacgcattga gttaccccgg
    21721 tgatgaaggc ccattggttg gattaactct tttgttagga cttggcattg ttgctactct
    21781 gttattgttt gacgccgctt tttctccttt tggcacgact ttagtttata ccgcagctac
    21841 atctcgtatt ttgtatggca tggcattaaa ccaacatttg cccaagtttt tcttaaaggt
    21901 caaccgccat aacatacctt acgtaacgct ttatgcaaat cttttggttg gttcttttgc
    21961 atttttaccc tttccgggat ggcaaaaact agtggcattt ctttcatcag caagtatttt
    22021 atcctacgga attggcccga tttgtctctt ggccatgcgt aaaatgcacc cccatatgga
    22081 tagacccttc aagctcgcaa atagtatttt cttgtcacat atcgcctttt atatatgtaa
    22141 tctcatgttg tactggtgtg gtttttctat tatttggaag ctaaacttag cattgcttat
    22201 agggcttggt atttactttt tttaccatcg ccatatttct ttgagaaatg gctcgtctct
    22261 ggtgtggttt atcatgtaca tgggatcatt ttctattatt tcttatttag gatcttttgg
    22321 cggcattggt ttactgaaat tccctttcga tttaatctgc ttcttgcctt atagcatttt
    22381 aatactttat atgtctcaag ttttagcgac accccgtcat gacaatgaca tgattggtaa
    22441 tgttacagaa ttgccagtat acaattcttg aggtagcaaa gtgacaaaat aaaaccgctt
    22501 cctgattatc cttgtctgtt ttgtagttca ctgtcatgac ttcttatggg taaaatgcat
    22561 caagggtaac ttgcctgctt cctgccaagg taccctgatg catattgagt tcccaacatt
    22621 ttttacgata aagcgatcgc atcagtataa gattcgtcta agttccgctt cctgtaattg
    22681 agctgccaaa gactcatcga actccaaacc ggtttgcact ttgccagaaa cttgagtatt
    22741 ccttttattc tgctcataag taaagaaacc ataaggcgat aaggtatcaa acagcgtttc
    22801 aaaatgttgg cggtaacgtt ttgttctctc ggattgagtt tcggttctga aggccctgtc
    22861 aagagtttgc tcttctttta aaggaacgac tctgtcagat tcagtttcat aaccaaaagc
    22921 tcgcttacgg ggtgtgctgt attgtctggg tacctgaaga gattctactt gtgttatcca
    22981 atgtgtatta ctcaggttct ttagagtgac cgtaggatat tccggaatag gtttgccatt
    23041 cggttgtcca acaacattga ggttgatttt aagtaggtcg gcaatttctt tcaaatcatc
    23101 atgcgttgcc catcgtcctt gttctttaat tttttcaagg ttcttcaaaa taattgaatt
    23161 tgaattgatt gtattgcctg atagaacatc gtttagtaag acttctttaa caaaagcatt
    23221 ttctattttc tgtacctgat gaaaaggctg gtttcttagg attggtctca gtattctggc
    23281 aatatcttgc gctgtttgaa caatttccgg agagagggcc agttcattga attgacttat
    23341 ccttgccagc gagtctcttc gatagagata aagttgtacc aattccatga atttgcaata
    23401 agcatttgag ccttctattt tggtttgatc tttacttcga ccttcttcga ttattctttt
    23461 gaccaaatct tctttattca caatgactgc aatatttcgt aaagacatct gcaataggaa
    23521 taataattca cttttataag tgtaggggga atcatgaagt tttgttagat cgacattaag
    23581 gatgtcttgc tgtcgtacgc cataaatacc ctcgacttgc ttccatcgat cataagtttt
    23641 actttttccg tgaagtttgt attcattttg tatgatgctt attaaaccaa tagcaagagc
    23701 ataaaatccg caatccccgc caccaggatt atctattgaa tatgacatct gtacactccc
    23761 ttttaggatt aatgatttaa tgtttcagtc aagcattata aaatatgaac attaagtaaa
    23821 cattatgtaa catatgccaa gggggagtgc aagtagctaa caattatctt ttgcctcgcc
    23881 tgtgggtaag caagctattt atcaagtggg tctccaattg acatccgtta ttattgcgtg
    23941 gaagaaaaaa atctcgctaa agccgtagcc tcttgtaaat tgacttctgc attaatccaa
    24001 tttaacttga cttgcgctgt tgtttgttca cgttgattca ctaaaaaaag cgtgctatca
    24061 ccttcataaa atctcttggt ttcccccttc tctacttgcc gtgcaagatt taactcttta
    24121 ttaagcaaaa ccacttgctg ctgatatatt cttattgcaa ccaagagatt ggacagctcg
    24181 ttatttaact gttcgtaggt aaacttcatt tccgttttta cttgctgtaa ttcactcgtg
    24241 gtgctgataa gctttcctct tgcttcacgt tgaaaaatag gaaatttaaa attcacgccg
    24301 atcatagcag cctgagggat caataaagga taaccgcctg ttccatattg tttaaaggta
    24361 taagctgtcg catcaagatt aggcagaagc tcatttcttg ctaaattttg ttttaattta
    24421 acaatttttg aataattctc caatttacgc aaaactggat gccgttgtaa ctgtgaaata
    24481 ctattggata ctcgaattgg ttgggcagga aatgttgccg gtaattgact ttcatccggt
    24541 ttttgcggtt tgccctttgc gtcacgatag taaagtgaaa gattgatgcc cgcctgtgcc
    24601 aataacattt ctccttgatt gaccagttgt tcacgttgaa caataagttg taaattttct
    24661 gcaatggcaa gtctaggcaa atcaccttgt tgagcttgtt gaataatcgc attttgccgc
    24721 tctttcgcta aattcagcaa atgcctgaaa gttcttaatt ggaaacccgc ctcaacccat
    24781 tgccaatagg ctttaatagt ttcctgataa attttaattt ttgtagcggc agcatcctgc
    24841 tgtttcatgc gaattgtttc ttctcttgta aaaagctcgg tacgctcttt atcaattcgc
    24901 ctgtctctca gcaaaggtaa agaaaggccc gcacgatact caccaccaga gttagtcagg
    24961 taattttgat agtaaatagg ccaatcacct ctaccaatac gatagcctcc aaacaattta
    25021 agtccattat agagggttgg cacattaaaa agattatctg cgtaattatt aatatacccc
    25081 ccgaccggct ggaagcgggg attcacatcg aaagaggggt caaattttcc taatgcattg
    25141 acataatcgc cttccgcttt tctgatttca agacgcgcta tgactatctg tgggtaactt
    25201 cgatcaacac ttttaagtac ggcatcaagc agcaaaggtt tgcctggtat tggaaaactc
    25261 ttgaatgaca acatcaagag caaacataag acaaccaatt tgtttttgcc tgaaggaaaa
    25321 caaatcatga tgctttattc tcgcgatcac tttccggcgg gaaaccatta tactgacgcc
    25381 aaagctcata ccataaagga acttggccca attgaaccca gccatgcacc cgcactcctt
    25441 gccgtaaaaa acgcacacta ggccatggct cattaggaac aatcactacg cggaataaac
    25501 caaacccatt atctgtagga tcaataaagg agactgtacc tccaaatgtt ccgactgcaa
    25561 tttctggcca tccgcgaaat tgaatagcag gccatccctc aaattgcaaa cgggcttttt
    25621 gattaaggcg tacgaaaggt atgtcattgc cgtcaaccca taattcaacg gcgcgagact
    25681 ctgtttcagg cagaatttga gccaatacat cgcctgcgtt gacaacgaca ctttcctgac
    25741 ctgtcaaacg tctaaagatg atacctgcga catgggcctt aatgatttgt gttttttgac
    25801 gagcaattcg gacatcgaca tttactttgt caatattcgc ctgcgcaagg tcattttggt
    25861 attttgcata ttcaatttgt gccaattcat attgccttct cgagttaatt ccctgttcgt
    25921 acaatttctt ttgtctgtca acattggctt tacctgcagc aatagcttgt ttggctgctt
    25981 ctatacgaag caaaattgcc tttttctcga cttcaagacg tgacaaaagc tccggatcat
    26041 tatcggtaat atcgactatt ggatcccctg gtttaacacg catgccttcg tcgacatacc
    26101 atttctttat tcgcccgcta acaggtgaat taaccgtatg ttgcctctct gttggtgaaa
    26161 aggcgatgac ttttccgttg cctaatgcaa actgttgcca cggagtaaaa cttaagaaaa
    26221 gaataatgcc aataaaaaca atgaaaatta ttttcgctat tttttgaggc ttgggaagtt
    26281 tactaatcat agaataagca tgtaatttca tgaggaaatc accaggcggt tatttaaata
    26341 gttaaaatcg gaccgttggc tcacgatcac tagtagcgta ttttttaaag acaataccaa
    26401 ttccatcatc tcattcactt cttgatgatt aaggatgtca aaagcgcgat caataacaat
    26461 taattcaggt tgtaatagaa gagccctgac caacatcaat tttgtgagct caactgaggt
    26521 aaacacatcc tgccattcat agacaatggt gtgtagacct tccggcagtt gtatgatttt
    26581 atcagcaagt ccaacattct gtaattgctc aataatgatt ttgttagaca tattgcgatg
    26641 attgagtact aaattgtcat aaatgcttcc ggtaaaccac tggggttcag caatcaataa
    26701 actaaaacga cgaagagaaa tgagggttct tttcgcacaa ggaatctcgt taatgagaac
    26761 atgaagtgac acaggatctt ggaacccaaa tagttgctca acaaacaatt ggcataaatc
    26821 tgatttttcc gaaaacacca ataaaggctt atcaactgct atcaatgcct tattcttgct
    26881 atccatctct aactggatgc tattaattgg ggtcaataac tcgccgtgct catcttcttt
    26941 cacctgttca agtgacaggt taagtactgc atcgatttta ttttctgagg ccaccagatc
    27001 ataataattt tctaaaaggg caccaaagcg tctaaacgag taaatcagag cacctaaaac
    27061 gatttctgcg gcaaccaatt gtcccagact taactgatta ttaataacaa gatagccccc
    27121 caatcccaat aaaaggctgc tggccaaagc agacagcgtg taaaaaccga tttgatgttt
    27181 gataagttgc ttaaaatggg tatttcttgc ttttaagaaa gcaaccaatt ttttatccgt
    27241 ttgttgagtc acataacggt gatgtaaatt aaaacggaac agataccggt taatcaaaat
    27301 ttcctctaac caagcaccta cgacgtgttt ttcagagcat tcttttttgg cgctctctaa
    27361 tgctttttta taggggataa aaatgatcaa tagaatgcca agaatgatga aaatatcaaa
    27421 caaaaggaaa agagggtgat aaattagaat cagcaatagt ccaaaaaaaa gttgtaaccc
    27481 caaattaatg ccatataaca ataaactcgc cagtgatttt ttgatagtga ctatttcaaa
    27541 aaatcgattg accaactccg gtccatgatg tgtcgaaaaa ttatcaagcg acatgtgggt
    27601 aaaatgtcgt gtcagactta aactgacttt aaccatcagt ttttgctgaa ttacttcaat
    27661 aattacaatt tgccatatat ggagagcacc tagagccatc atcaaaatca ataccatcaa
    27721 acttaatgta atgacgggtt gcaatagctt gccaaaagca attaaattca caagtgtttg
    27781 ggcagcaatg gggatactca atgacagtaa gctgataatc acactaatca tcgcgatgga
    27841 tgagattgtt tggcgatcta atacatcaat aattgaatta attggttttg cattcattcc
    27901 ctaaacatcc cttttaccct tgattcttgt ttatgctatt aatgttttaa tttttgcagt
    27961 ataaaaaact caagcgattc aattgcttta ttgctttcac tcttttttaa taaagagaca
    28021 tggacatcat tcaaaacagg gagaaaatcg taatcgagcc gttccagata gtttgggatc
    28081 atgctgcgct gaatagcagt aatccctaac ccggcgcgca cagctgccat tttccccgca
    28141 taacttgcgc tactgtaaac aaggcgccaa tcaatatggt ttttatccag tgtatcaatc
    28201 acattgcctc gataaacgca gggtgttggt gaaagcacta agggaattgg ccccggtttg
    28261 tctaaaagag gtaataattc ttttttccct atccactcaa cgggttctgt gcaaacatgg
    28321 atagactcag aaccaacctg ttgctgagtc gttttaatga gaattaagtc aaatttacct
    28381 tcattaaaac gctcgatcag attcagagtc aaatcacatt ctacattcaa taagactcgt
    28441 ggatgcaatc ttgaaaattc aacaagtacg tccgataaaa taatactggc aaaatcctct
    28501 ggtaatccga agcgtatttc accttgtaac tcaggcgctt taaaacgatc caaagattct
    28561 cgatgcaatt cataaattcg tttggcatac cctaaaaata tttcaccgtc cgtggtcaga
    28621 gaaagttctc ggccacgatt aattagaggt ttttcaatca ggttttccaa tttagctatt
    28681 tgctgactga tagccgattg ggtgcgtcca acccgctgtg ccgccttggt aaaactttgc
    28741 gtttcagcca ctgccagaaa gcattgtagt gtgattgtat caattgacat aagtttttct
    28801 aatagtttaa atcattattt ttaattttac ttatattaac atatctaata tatttccaca
    28861 ataataacag attttctaat ggagaattaa tgtggataaa cttttaaatg ggttatcgat
    28921 ttcactcatg ctatcaccct tgatagcgat gctattctat agcttgttag gaaaaacaag
    28981 aaatattcaa gcaacttatt acggtaccac agttataggt tttggttttg gattgagtct
    29041 cttggcatta attgtttctg caaatactgt aaaccaaaac agttgttttt tatttcatgc
    29101 cagcacacta agcctcttgc taagcacatt aatattattc ataagcttta ttatccaccg
    29161 tttttcccta cgatacatgc atggggacaa gtgctaccga cgttattttt tcaaactttc
    29221 agccataact ttaacaactg ttgtgatggc tttagcagac aatcttttat tgttctggct
    29281 tgcctggtca gctagtaact tgctcttgat aaacctcatg attcataagt cacaatggaa
    29341 agcagcctac aactcaggga tactgacttt aaaaacatta ttaccaggaa gcttgttgct
    29401 tatcactgca ttttggattc ttttcacatt aaacaatact ctgtcaattg aaacaataac
    29461 cagcgaagtg agcaataagt catcccctgc tattgctgtg gcgcttggat ttattatctt
    29521 aacagcgatg acacaatcag ccatatggcc ttttcatcgc tggctaatga gctcattaaa
    29581 ctctccaaca ccggtatccg ctctaatgca tgctggatta gttaatggag ggggctttct
    29641 gattgttaaa tttgctccgc tgttcgtagc gaaaggtaat ttattgacca ttttatttat
    29701 tttcggcgca ctgactgctt tattaggcac cctctggaaa ctattgcagt ggaatatcaa
    29761 aagtatgctt gccagttcca ccatggcaca aatgggtttt atgatgatgc aatgtggctt
    29821 aggacttttc cctgccgcta tcgcccatct atgctggcat ggcttgttta aatcctatct
    29881 ttttctcaat tcgggctctg ccgtccaaca aaagaaatat caaaataaat cacgtcccga
    29941 aaaattgaca accttattga ctgcattttt tggaggactt ggtggaatgt atggctttgc
    30001 ccttatcaca gggaaaccca tcttatctct ccatgcctcc acctttcttt taggttttgc
    30061 attcatcaca ggaacacaac tcaccctggg cttgattgca tatgagaagc atttcaaaaa
    30121 agtaatttta gcctgggctt tagttttctc aattggaatt ttttatggag gaagtatcca
    30181 cctcatcgag tcactcttac ctaatctaac aggatttcct caataccaat taagcggctt
    30241 acaagtctta actctgatta tcttcttttc cctttggctt gtattcagca tggggttatt
    30301 caaaaaaatc agagagacca gactttggga ttggttttac atgtacatgt ttaacgcaag
    30361 tacgcctcac cccaaaacca ttacggcaaa tcgccatact tattactatt aagaaaagga
    30421 gccaacatga gtagccacat cacactaatc aaaactaaca caaacaatga aaataaaatg
    30481 acccaattta actacaaagt tgataatgat gtcatgatta tccgggctat ggtaaataat
    30541 gttgcaaaac aaatgacacc agtatggcct ctggaaaaat ttattgcctg caatgccttg
    30601 catggttttg aatccatgtc ctttgaagag gctgtgatac aaaaccaaac ggcaaaaaaa
    30661 ggcacgccat tcaatgaaaa actggaaaga gtcaattggc acatgatcaa atggtgtggc
    30721 agctttctgg acattggaca aggcacgctt gaaatgcctc atcgtgacaa aggcttatat
    30781 tttggttttt taaagctagc cccttttgac agcgcactgc atcaaaacag taaatcaata
    30841 aagagctggt tatcaaacct gccagaaatg cctgaacaag ctatccgact ttgcctcgac
    30901 aaacttggtg tcttaaacca aaagcaagag gattttctcc aatccactct gtctcactta
    30961 cctggatggg ctggatacat taagtggata agtgaatgga aaaatcgcaa tggcaaggaa
    31021 gagaatcctg ttagccttgt cgattttatt gcagtgaggc tggtaatcac ctgtatatta
    31081 tggccggaag caagccagga agaaaaaaaa aagaaaaaag atagtgcaga tactaaacaa
    31141 cttattcaaa atataaaaaa taaagaagac gattacaggc aattgctttt aaaaaagctt
    31201 ttgcctgagt taagcaaagc tcatataaaa gaaaatagag cccatgccca aatggttttt
    31261 tgtattgatg tgcgctcaga accgtttcgg cgatgtatag aaaaactcgg acactacgag
    31321 acgttgggat ttgcaggatt ctttggttta cctgtgagca tcaaggatta tgacggtgaa
    31381 acaataaaag actcttgccc agtcctttta aaaccacgat ttaatatcca tgaaaaagca
    31441 attgctgcaa atgaacactg catagaacat catgagaaag gcaaagaatt taaaaatatt
    31501 ttgaacaggg tctatcagca attaaagtat aatttttcaa ccccatttgc tctggtagaa
    31561 tcgttaggaa tttggtgtgg gattactatg ttccttaaat catgtagccc aattttcgct
    31621 agacgcctta ccaaagacct aaacgaaatg atatgcccct ccattcaaac acagcctgtt
    31681 tttgaattgg atttattaga gaaagaggta ggtatctcac ttcaagaaca aattgcctat
    31741 gctgaaatgg cattaaggct tatgggactg actgataact ttgccaaact tgttattttc
    31801 tgtggtcatg gcagttctac tcaaaataac ccttatgctt cggccctgga ttgcggtgct
    31861 tgtggaggta atcaaggtgg aaaaaatgcg caattactgg cgtctatcct caataaaata
    31921 atagtgagaa gggctctggc agaaaatgga atcaatattc ctcaagatac cctgttttat
    31981 ggcgcgcaac atgacacgac gacagatgag gttgaaattt atcactcaaa tgtcagccaa
    32041 ttcattcacc aagatatcct ggaccagctt agaactgact taaacatggc taagtacaat
    32101 aacaatttag aacgaattaa ctatttgaat tccatagatt gcgcagagaa agacattgca
    32161 cgaagaagca cagattggtc tgaaacaagg ccagagtggg ggcttgctcg caatgccgct
    32221 ttcatcgtag catctcgcca actgaccaaa aacattaatc tggaaggacg ttgtttctta
    32281 cattcttatg actggtccca agacaaagat ggtactttgc tggaaaccat tttgactgca
    32341 cctatggtgg ttgcccaatg gattaatact caatacctgt tttcaacaat cgacaacgtg
    32401 gcatatggaa gtggcagtaa aatcactcat aatgtcgcag gaaaaattgg agttatgcaa
    32461 ggcaatgcca gtgatttaat gcatggattg ccactgcaat ctgtcatgag ccatgatgag
    32521 caatcctttc atgagccaca acgtctgtta actgtagtct atgctcctcg cgaaatcatc
    32581 agtgaactag ttgaaaaaca cgatgtctta aaaaccttgt tttttaatga atgggttcat
    32641 ttggtagcga ttgatccgcg gaatcacttg ttttacaagt tagagaaaac caatacctgg
    32701 tctgtaattc agtgaaagga gtttcctgat gaatataatc aaagtatcag aaaaaaaaga
    32761 gatgaacatc tctttgcatc cgatgaaaaa gatcgaagtg atcgtatctg gtgacaatga
    32821 aaatatggtc gctactatga tggagaaggc aaatatttct ggttttacct tattgcgaaa
    32881 catctctgga aaaggacatc atgggtttca tgaaggtaaa attttgttta acgacaaagc
    32941 ctctttcgtc atgtttattg cagtggcacc tgaggaagct attgctaata ttgcgcttgg
    33001 catgaaaact ctattccaaa agaattcagg agtcctgttt gtgtcggatg tttctgttgc
    33061 tagagtcgat tatttctctt agtatttaac tttggaggtg acccctatgc actatcaaaa
    33121 tcagcgtgtt ttactggcct caaaacatca aaaggaaaaa gcgattcagc caccttttga
    33181 ggagaaatta ggatgtcgta tccatgtccc tgctgattat gatactgatc agttcggcac
    33241 attcaccggt gaaataccaa gaaaagcctc agcctatgaa acattaattc tgaaagctaa
    33301 agcagcagcc aagcaatttg gataccgtta tgtaatatcc agcgaaggga gtttcggccc
    33361 ccatccccag ctttattttt gtcccgcaga caccgaattg atggcctttg ttgacttgga
    33421 taatgattta attatcgcgg agcacgaagt atctacagag acaaattata gtcatattga
    33481 tatcagtgcc acagatgact atgaagcgtt tctcaacaaa gcaaaatttc caagtcatgg
    33541 cattattgtt cgagccttaa atagcgaaga aaattatatc caaaaaggaa tctgtgagcg
    33601 tgagcaactt aaaacagcaa ttcaaaaagc gttcacatac agtacaacta tccgccttga
    33661 aaccgatatg agagcgatga tgaatccgct gcggatgaat gtaattaata aacttgcggt
    33721 gaaactggtt caccgcattc agcaatattg ccctcaatgc cacactccgg ggtttggaaa
    33781 aatctcgtct gaaggtcatt tagcctgcat ttcttgtgga acaccaacag aactttacct
    33841 gcgaaaagtt ttaaattgtc taaaatgtga atttaagact tatcaagcgc gagacgatgg
    33901 actagaattt gctgaccaaa ggtattgtcc ttattgtaat ccttaaccgt atgcgagaat
    33961 caagtccatg gtatgttata aacattcaaa caagagaggc acactatgaa acccacttta
    34021 ataggtctaa ttatgggctc tcaaagtgat tggcaaacat taatccatgc cgcccaaaca
    34081 ctggatgcgc taaacattag ctatgaggca gaaattgtct ctgctcatag gacacccgac
    34141 aaacttttcc agtatgcgga acaggctgag acgagaggtt tggaagtgat tattgctggt
    34201 gcaggaggag cggctcatct ccctggcatg gtagcagcca aaacatcttt gcctgtttta
    34261 ggtgtcccag tcatgtcaca aactttaaac ggcgtcgatt ccttgctatc cattgtgcaa
    34321 atgcccgcgg ggatacctgt aggcacttta tcgataggta aagcaggtgc catcaactct
    34381 gcactgtttg cagctgcaat tcttgctaat aaatatcctg acattcaagc tgccttaaaa
    34441 cgctatcgag aacaacaaac acaaaaagta ctcgacaatc caaaccctaa agaggaaaag
    34501 tcatgaaact tggtattcta ggtggtggcc aacttgcaag aatgttggca ttagcagcat
    34561 atcccttagg gattaacact gtcagcctgg atcccagccc tgacgcctgt gctggtcaac
    34621 tcaccaggct gatagtctct gattttaatg atgaattggc tttaaaacaa tttttgtcag
    34681 aagtggattg cgtcacaata gaaaccgaaa atataccatt ttcttgcgcc gaattggctg
    34741 cccaaataag gcctttctat ccatcaaccc aagctttgaa aattacgcaa gacagattgt
    34801 atgaaaaaaa ttttttcaga actctgaata ttcctgtacc agaattttta ccggttgaat
    34861 cagaagatga attgatgcag gctatttcag tcataggaat gcctgctgtg ctaaaaacaa
    34921 gaaggtttgg ctatgatggc aagggccaat atgttttgcg aacccaagct gatatttcaa
    34981 aaagttgggg cctgttaggc agccaagctc tcattcttga aaaattaatc tcctttgaaa
    35041 gcgaattttc gttaattgct gttcgtaata agcgtggtga aacaagtttc tacccattga
    35101 ttaaaaatca tcacaggaag ggcattttac atttctcgga agcccctgtt catcaggaca
    35161 ccttacaaca agaggcgcaa actcatgcat tcaaaatttt agaagccctg aattatgttg
    35221 gtgtacttac cattgagttt ttttatcaag gcaaccaatt gattgcgaat gagatagcac
    35281 cacgcgttca taattctggc cactggacta tcgagggagc acatactagc caattcgaaa
    35341 atcatctgcg cgctcttttt gatttgccct tgggaagcac tgaagtaagc agctcttgtt
    35401 ttttattaaa ttgcattggc aatctcctgc cattggagcc ctgtttatcg atcccgggcg
    35461 ttcattatca cgcttatgga aaagcacccc ggccagatcg caaggtaggt catgttaccc
    35521 ttatcgatac tcatttggaa cgttatgcaa aaagtaaaga cttactgctt ggcatttcat
    35581 gagtagccga tagctagaaa gcgagcaata ggagtcagcg cataatattg ttcgcaattt
    35641 ggaaagctga gctttggatt ctgtttccta ctgctcatcc acattatcct gcttattatt
    35701 gcttgccact atagactcag gatcaatgga gcttccggga atttttcggc tttcgctggc
    35761 ccattctccc agatctatta acttgcatcg ttcagagcaa aaaggcctga actggttatc
    35821 agggcgccag gtattttgct tgccgcaaat ggggcatttg attttttgct ggttgttcat
    35881 tccggttttg ctaatttata tagtttagat atgaagtatg gctacaccag caatgatttt
    35941 caatacacct gccttatttg gataatagga aaatagatct cttgcattcc ccaatctaaa
    36001 actaattatc aacatgcact tcgcgcacca gagccaacca ggcttgcgca gcctcggaga
    36061 gataagagtt tctgcgccag gtcatcgcca tattccattg gaactcactt ccattaagta
    36121 aaagagagcg aatttgtgtc gtatgccttt gagtggcaat catccttggt aaaaaagtaa
    36181 ccccaagacc tgccgcaaca agctccagca taaaatcaat ctgactgctt tgagccacca
    36241 ccttaggttc gaaaccagcg tgacggcacg cgtccaatat aattcgatgt agtgcgaaac
    36301 cactgccaaa aaggatgaaa ggtatttctt taagttctgg tagagaaatg caatgtttag
    36361 ttgctaatgg atgtccagat gccaggagcg cgaccaacgg ttcagagcgt actaattggc
    36421 aatcaaattc ttcggaaatg ggtaataagg ttccagcaaa atctatctct cctgcacgca
    36481 aacactctgc aagtttatcg ctgccatgtt caacaagctc tacttcaatg cctggataac
    36541 gttgtctgta cagaacaaaa agaggggcaa ataaagtact gctgcccacc ggagaaatac
    36601 ccaaacgcag tcgaccttgt ttcaaacctc ggatttcatc caattcttta agaagatcat
    36661 ctcgatcagc cagtagtttg gttcctcgct gataaacaat ttcacctgct gttgttggta
    36721 cattacgctt tttaaaccgc tcaatcagag tgatacctaa ctcctcttca agctgtttta
    36781 tggctttact taccgtagac tgggtcgcaa atagattttc ggctgctttg gaaaaactac
    36841 cctgccgtac aacctctatg aaagtctgaa gcagtttaaa ttccataatt attccatatt
    36901 ggaaaggtta ttagtctata aattcatttt attcatctta attaaaagtg taaacttaaa
    36961 tgaaaaaata atatccatca tgagcatata tatggcaaac aaaattcagc aaaattcatt
    37021 ttttcagata atattggttg gcttattttg gttaactagt gagttattgc tcaaactagt
    37081 caagcttcct ttgtccggag gaatccttgg attaggaatt gttttactgc ttttggcgac
    37141 gaactgctta aaattaaatc gtataagacg gggggctgag ttactccttg ccaatatgtt
    37201 actttttttt atccctggcg tccttgcaat actggagcac caggaattta tcggtctttt
    37261 aggccttaaa attctcttta ttattctgct cagcacaatc acggtgatgc tagtcactgc
    37321 ccttgttgta gattattgct accgttggag aacgcatcat gccaagcagc attctctctg
    37381 aatccatgat ccaggcattt ttctggtcgc ttatcacaat aggcatgtat tttattaccc
    37441 aaaaactata tagaagatgg cccttttggt ggctaatgcc gcttgcttta acaccaatac
    37501 tggttgccag cgtagtgctt ttactgcatg taaactacca ggactatttc cgcggaacaa
    37561 aatggctagt cttgttaatc ggtcctgcaa ccgtagcctt tgccattccc atttatgaaa
    37621 aacgtgccct cattcgccag cattggccag tacttttaat tggcgtgatt gtgggaagtt
    37681 tcactgccat agtatccagt tggggattag caatccttct tggccttgat gataatttac
    37741 gtctaagtct gctccctcgc tctatcagca caccgttcgc tatagaaatc tcgcggggaa
    37801 ttggtgcagc gcctgatttg actgcgattt ttgttattgt cacagggata ttgggagcta
    37861 tactcggtga gttcatcctg tcgcgcttgc cttttaactc agctcttgcc agaggtgccc
    37921 ttttaggggt aggagcccat gcggcaggaa ccgctcaggc ccataaaata gggaatactg
    37981 aaggcgccat tgccagtttg gctatgattt tagtaggact cttcaatata cttcttctcc
    38041 cccttatcag ccatcttttg aaataaaagt gcgtttagta gaaattaaaa ggaactcctc
    38101 tagttaagaa aaaataataa gcatgttatt ttatggtttt tccatcaata gaagaaaaga
    38161 atgactattg ctcttgtgtg gttccgtcaa gatttgagat tgaccgataa tcccgcgttt
    38221 atcgaagcat gctcccatca tgaaattgta attccacttt atatttatga tgataaatgc
    38281 agtgttttag ggcaagctca agcctggtgg ctccatcata gtctcattgc tctgggcaaa
    38341 tcgttaaatc aacagggttt aagcctcgtt cttcgcaaag gagccccgca agagatcatt
    38401 ttggagttag tcgcacagta tggtgtggaa tcagtctatt ggaatcgctg ctatgaacca
    38461 caggccattg cacgtgataa aaaaattaag cttgctttgg gcgaaagaga aattaaagtg
    38521 cagagtttca atggaaattt gcttcatgag ccgcgggcaa ttaaaaataa aaatggggat
    38581 ttttttaagg tttttactcc ttactggaaa caatgtcgac aaattcttaa tatcccctcg
    38641 ccttttatgc tcactaaacg cccaagggga atcgaaatta caagtgatga ccttggcaaa
    38701 tggaaattat tgccagcaat taattgggca gcacgatttt ctgaatattg gattccagga
    38761 gaagagggcg cacaaaaaaa actggatgag tttattgttc atcaccttag cggatataaa
    38821 agggagcgcg attttccagc taaaaatgcg acatctcgat tgtcacctca tttgcacttt
    38881 ggggaaatca gtccttgggt gattttaaga gcactggagt tggctaaact ggaacaaacc
    38941 tgtgatctgg cctccgtaga acatttttta tcagagctgg ggtggagaga gttttctgtc
    39001 tatctgctct accacttccc caagctggat tgcgaaaatt ttagaaagga gtttgatgca
    39061 ttcccctggc aaaatgatga gcaattatta acttgctggc aaaagggaat gactgggtat
    39121 cctatcattg atgctggaat gagagaatta tgggccacag gatacatgca caaccgcgtt
    39181 cgcatgattg tcgcatcatt tcttaccaaa gatttactga ttgattggcg ttcgggcgca
    39241 cagtggtttt tagacacact agtcgatgcg gatctggcga ataacagcgc gagctggcaa
    39301 tgggtagcag gatgcggtgc ggatgcagca ccttatttcc gtattttcaa tcctgtttta
    39361 caaagccaaa aatttgaccc ggatggcgtt tacatacgcc aatggatccc cgaacttgcc
    39421 caattaaata gtcagtcaat tcatcaaccc tggacagatg ctgacgccag ttccattttt
    39481 ttagggaaaa aatatcccaa acccatcatt gatcaccaag aagcgagaac taaggcattg
    39541 agttactatc agcaaataaa aaaaggaatg gattgacaag agattaacta aaatttggag
    39601 atacccgatt gaacaaaaaa agttggacgg agctgattgc ctggatcatt acctttgaag
    39661 caattggttt ttttttagga atgctgacgc aagcgaatat tccctcttgg tatgaggggc
    39721 tcaataaatc aggtttaact cctcctggac cagtattttc aattgtttgg tcgattttat
    39781 atgcccttct tgcaatagtt ggttgggttt tatggtgctc tcgaaatgag tctgagatga
    39841 gatctgtgtt ttatttatat tcagtacaga tgctgatgaa ttgggcatgg accccgcttt
    39901 tttttgtgct gcattggact gggcttgggt tattatggat tcttgtgatg gcgggtctca
    39961 ctgggcttct gattttgagc ctaaaaaata agaaaaaaag catcgctata ttgttactcc
    40021 cctatctcgt ttggcttctt tttgccgcct atctcaatgg cgtgatatgg cttctcaatt
    40081 aactgttccc aagttttccc ttaagccaaa tacaaactgg ctatagcgaa atcgtgctca
    40141 atcttacttt gcgagataag tacaggactt ctaaataact cccatcatct ttgagccaac
    40201 agaatatatt gccctctgaa gaattagggt tgagggcagt tggaaatcca atccatagta
    40261 ttatcttccg cgacattgta aatatccgaa ttattaacaa taagagaatc ttgtaacgca
    40321 gaagtgagtt ggcaatgttt tgcgccgtgc ttgactaaaa ataaaagagg ggacttcctt
    40381 tgctgatctt tttttaagat tttggctagg gccgactcac caaaatcatc aacagcatca
    40441 acagagccga catgttgctg gcaaaattta attagcaaat cactcatcaa gggatattcc
    40501 tcagatttgt tcgcaatggt cttgattgcc ttgggaaaga ggttcttttg ccatggtgag
    40561 tacaaggctg acattaattt ttgcgcaaca cgatctgctc ctgaatctaa ttcagcaatt
    40621 agcatgctga tttgctcatc ataactacca gtagcttggg ataaaaaatc taaaaagagc
    40681 tgttcgtaat ctttttcggc ataaaattcc gctgtcatgg gagtatctgc tttatcactc
    40741 gccaaaaagc gcttaacagg tttatctagg tttaagaaaa ttcgattttt gataattgct
    40801 gcatttttca tatcacaaa
//
LOCUS       DBNCWK010000016        37492 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.016, whole genome shotgun sequence.
ACCESSION   DBNCWK010000016 DBNCWK010000000
VERSION     DBNCWK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 37492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37492
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.016"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            225..1157
                     /gene="glsA"
                     /locus_tag="AC06IO_000715"
     CDS             225..1157
                     /gene="glsA"
                     /locus_tag="AC06IO_000715"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HIO9873928.1"
                     /translation="MSSKLLTIQLLEELVHAAELNQEGKTADYIPELANVNQELTAIA
                     VQALGEKTLAYSNNPLHPVTLQSTGKMIPLIGLLEEFGADQLFEWVKVEPSGDDFASI
                     TRLEQFGPKPSNPMLNAGAIALCSRIPGVGEQQFRWLEHWVQKLFNQRLSINPLVFAS
                     EKRTGNRNRALAYLLKSRNNLGADVHETLDLYFALCSYEAMLDQMLYLPTLLANSGKD
                     SDTGEQILSMETCKITLAIMATCGLYDETGTHMVKTGMPAKSGVSGYTIAVVPGKAGI
                     VVLSPRVNAKGNSIRGEIMLEGLSKAMNWHFALP"
     gene            1281..2168
                     /locus_tag="AC06IO_000716"
     CDS             1281..2168
                     /locus_tag="AC06IO_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9873929.1"
                     /translation="MKIAVIEPIGIAMQEIRFELPGHTIIECDSRQWSDEQLIDFVKD
                     ADIIALTNRRLSAAVINSALRLQLIAVAFAGIDHIDKDAVSKRNIPVKNAAGYANTAV
                     SELVFGLMISLARHIPDNNQRIREHGITNTGIELKNKVLGIVGYGAIGAEVARLAKAF
                     QMTTIIYDRKSQVTLDDLFSQSDFVTLHVPLTNETKGMVNLRFLSQMKKSAYLINCAR
                     GPIVVSSDLKKALEQGMIAGAALDVFDVEPPLPANYSLWEAPNLIATPHIGFNTSEAL
                     VAKGQLTIKNIKEFLSSRL"
     gene            complement(2456..3055)
                     /locus_tag="AC06IO_000717"
     CDS             complement(2456..3055)
                     /locus_tag="AC06IO_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HIO9873930.1"
                     /translation="MKIVVIGATGIIGKAIVAELNPRHEIISASYKNSDIQVDITSKQ
                     SIDAMYKKLNTIDAVVLATGKVHFAALKDMGEDDYKIGLTNKLMGQVNVVLAGLKYIN
                     DNGSFTLTSGILNRDPILYGSSAAMVNGALDGFIKSAALEMPRGIRINGVSPTVITEA
                     MENYGPYFRGYEPVSAARAALAYSKSIEGAQTGQVYCVG"
     gene            complement(3385..4779)
                     /locus_tag="AC06IO_000718"
     CDS             complement(3385..4779)
                     /locus_tag="AC06IO_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HIO9873931.1"
                     /translation="MVLEFDTIILGGGKGGKTLAMDLAKSGQKIAMVENNQIGGTCIN
                     VACIPTKTLVQSAKVAHYCRKAKDYGLNTTLSSIDFKAIRARKDAVVNGMREANLKQF
                     LDSGMDLMLGHGHFIGPKMIEVTLSSPRGNQKTLHITADKIIINTGALPYTPPIAGLD
                     KVNYFTNDSLMNTDSVPQHLLIIGGGYIGLEFAQMFRRFGAEVTVIEASSEFLGREDK
                     DIAEQVFQTLSNEGIQFAVDTKINAIRQEQTEVIIEANRQGQSEIIRGTAVLVAVGRI
                     ANTAGLHLDRTGVELDERGFIKVNEFLETTAAGIWALGDVKGGAQFTHLSLDDYRLVK
                     HNLQNPQKKLSSQNRLIPYTVFLDPELARIGLTEAQARSQGRPIKIATIPAAAIPRAK
                     TQGETTGVLKAVIDAETDLILGVSIFCAEAGEILGGIQLAMELRVPYQKLRDMMFAHP
                     TLVEGINLWFAQII"
     gene            complement(4831..8193)
                     /locus_tag="AC06IO_000719"
     CDS             complement(4831..8193)
                     /locus_tag="AC06IO_000719"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIO9873932.1"
                     /translation="MMTNSWKDKLQKQLTQQFGSPKGKQLIDKYTETMSMSYCEQHTP
                     EEAIQDILQIEKLSKDNPLVIDFYEKTHLQYPLHIKLYRYETPIPLSDILPMLENMGL
                     RTYTERPYKIKTRDGQLFWISDFNVTYSQTDSFNITQVNNIFAEALININLGICENDG
                     FNKLVLSAGLSWQEITILRAYAKYLHQIGIRYSQSYIEKTIEMHAAIARDLIHFFYLK
                     FGLKRKSAIQKDMSALEQKIQINIDAISSLDEDRIMRYFWSLMKATLRTNYFQKNHAG
                     LPKDYLSFKLKSSEIPDLPPGQPLYEIFVYSTRFEGIHLRSTKVARGGIRWSDRPEDF
                     RTEILGLMKAQKVKNAVIVPSGAKGGFVLKKPLHAASREQIQQEVISCYKSFINGLLD
                     LTDNLINDKTIPPQNVICYDDPDPYLVVAADKGTATFSDIANSIAKEHGFWLGDAFAS
                     GGSAGYDHKKMGITARGAWESVKRHFRELDINIQQQDFTVVGIGDMSGDVFGNGMLYS
                     KHIRLLAAFDHRHIFLDPNPDPVKSYEERLRLFNLPTSSWEDYNLQLISKGGGVYKRS
                     SKSIPISPQVKKALAIEANALTPNELIRALLKAPVDLLFNGGIGTYVKATTESHADVG
                     DKTNEFCRINGAELHCKVVGEGGNLGFTQLGRVEFALKGGLINTDAIDNSAGVNCSDH
                     EVNIKILLDKEIREKKLTEKKRNQLLSRMTDEVADLVLQDNYNQALILSISAAHSSHY
                     SGLYQDYMKELEKWVNLDRSVEFLPDDKKLLERKTTGAGLTRPELAVLMAHTKIHIAN
                     ELLKSHLPDDSYFTTFLETGFPISLRKPYAKALPKHPLSREIIATQLSNKLVNNMGIT
                     FMYRMLMETGMSIADIACAYTISACIFQTDVLQNLIDSFQDKISLSTQYELLHHIRQL
                     LNLATRWFLHNNRLTGGIEKNIKNYGKSVKKLEQLIPKLMGGVTKEYLEKLISQFVSI
                     GIEKDMAQKIAITRALYTSLNIIEVATQNEFDLSLTAETYFNVGSQFNLVWFRDQIAN
                     DSREGHWNTMARLSLRDELDNLQRRLTIAILTTNTKERTSEKLINYWLEQNHPIQKRW
                     EKLLEMLLGSENIDYTIFFIALRELSSLIQVL"
     gene            8391..9116
                     /gene="ceg9"
                     /locus_tag="AC06IO_000720"
     CDS             8391..9116
                     /gene="ceg9"
                     /locus_tag="AC06IO_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg9"
                     /protein_id="HIO9873933.1"
                     /translation="MYKNILFFSNFSRMRWNEYDGDRYQSLWEHNKRDIKHTFFGTIA
                     NGKDLREVLFQPVTASFSMLVAGAFAQLLVPYSIFILCMGEAGFAGKTLINYLEITTV
                     ALAMPFIALLSTVGRCIATVKCLVEDQYNLAEMVEDKEVKPNKIYLDCAEVNYTVRDE
                     NNNQFSRKYYKFQYKMRGLNDELCQGFFTFRDLERAPDDDDFNGKLSSLTLYKYDILD
                     LIKRIHKDKFEEMEDENLIRISI"
     gene            9349..10302
                     /locus_tag="AC06IO_000721"
     CDS             9349..10302
                     /locus_tag="AC06IO_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873934.1"
                     /translation="MRLSYGLLGFMIFNLVACEKMALLTTPPKKQQLSNSPLATRAER
                     YFWRTLHEGRYQDIPQADYLLMAAYLENPYDSKLAAHLGFIHIWKITERERVKNNSPL
                     LPNEIILSKKYFADALQLDPENPIYQGFYGDTQLVEGQIFKDKQEEVRGYFTLQKAIS
                     NWPEFNYFTAGYPMSSLSADSDHFKKGLDWQWKTLDICAGEKISRQNPDFTPYMKRET
                     TTGAQRACWNSEIAPHNFEGFFMNMGDMLVKSGDPETGIKIYNNAKLTKNYNKWPYKD
                     MLEKRILNAKANVQHFNQKSNDPNQSIMFNSGYGCVVCHQR"
     gene            10462..10803
                     /gene="arsC"
                     /locus_tag="AC06IO_000722"
     CDS             10462..10803
                     /gene="arsC"
                     /locus_tag="AC06IO_000722"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212942.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIO9873935.1"
                     /translation="MEKITIYHNPRCSKSRQTLEILQNKGFEPIIIEYLKTPLDLEQL
                     KRLRTHLALEDLVRTNENVFKELGLSLANEAQVLEAMVIEPILMQRPIVTFKGKAVIG
                     RPPEKVLELFD"
     gene            complement(10890..12044)
                     /locus_tag="AC06IO_000723"
     CDS             complement(10890..12044)
                     /locus_tag="AC06IO_000723"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIO9873936.1"
                     /translation="MMFGFLNMTLWGYLLTGFVLTHITTTAVTIYLHRYQTHRALTLH
                     PLVSHFFRFWLWLTTGMTTAEWVAIHRKHHANTDMEGDPHSPQVFNIRKVFWQVPELY
                     REASKDKDMIKKYSYGTPNDWIERKLYSPHANKGIFLMLALNLFLFGIPGLTLWAIQM
                     MWIPLVAGVVNGIGHYWGYRNHESSDSSTNVFPWGLLIAGEELHNNHHTFASSAKFSI
                     KWWEFDLGWFYIRCLSFLKLAKVKKLPPKLIIDKNKSHIDVDSVKAILGNRFQVISDY
                     YKLVIFPTLRDMQYRIKNRDLKNARHLLKKPNEQLSHADQLRLKTLLKQHDNLQAVHN
                     YRELLQQIWLKTSLSNKDLLNLIQKWCSQAEESGLLVLRQFASQLKSYSL"
     gene            complement(12163..13407)
                     /locus_tag="AC06IO_000724"
     CDS             complement(12163..13407)
                     /locus_tag="AC06IO_000724"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIO9873937.1"
                     /translation="MSFKQLALIEPLNRAVSELGYTTPTSIQLKAIPLILNGHDLLGS
                     AQTGTGKTASFVLPILQKAGQQTPASPNRVKVLILTPTRELAIQVHESIIQYGKYLTL
                     RSAVIYGGVKSHNQIKQLDSGLEILVATPGRLLDLYQQRAVKFDEIDTLVLDEADRML
                     DMGFIHDIKKIIKLLPLKRQNLLFSATFTPEVRALARNILNKAVEIDIAPRNTAVKTI
                     KQTVYSVDRNHKLALLSHLLHKNNWGQTLVFSRTKHGANKLVKQLAESQIYSVAIHGN
                     KSQAQRTKALADFKSGKVQTLIATDIAARGIDIEKLACVVNFDLPHVPEDYVHRIGRT
                     GRAGASGLAVSLVSTEEIKLLLSIEKLINQKLERIKIKDFEFLHNFSDLVSAKIQKFA
                     PPKAEYKGNISRKSRTAFNKSA"
     gene            13685..14362
                     /locus_tag="AC06IO_000725"
     CDS             13685..14362
                     /locus_tag="AC06IO_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HIO9873938.1"
                     /translation="MKAKGFFDEQSSLKKSKIGLNLVWMFSLGHIFLITLSNVLVQYP
                     FDMMGFHTTWGAFTYPAIFILTDLTTRIATANEARKIIMLSMTPGLILSYLVASYIEA
                     GNNLNESGLSILHLMPLRIALACFIAYVVGQLLDVAVFQRYRNRPAWWLAPTLSAVAG
                     NFIDTALFFTIAFYHCSNPFLSQNWPEIAMVDVFFKIMISLIAFVPVYGLVLSILGIK
                     ITNKLMV"
     gene            14401..15039
                     /locus_tag="AC06IO_000726"
     CDS             14401..15039
                     /locus_tag="AC06IO_000726"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9873939.1"
                     /translation="MLTGLRLLTKDLKILNGKYIQLEPITSQHREELRKAANHEQIWQ
                     YMPHKATGKLFNSWFDDCLEKMSIRDQITYAVRCKKEGDLKGATSFYDIQPENKRLAL
                     GYSWYIPKVWGTTTNPESKLLMLHQAFDAWGINRVELGTDSRNIHSYRAIKKLGATEE
                     GVLRQHMILHNQVITDTIMFSILFSEWPAVKERLIQRLNYAATDKDHLAASI"
     gene            15174..15701
                     /locus_tag="AC06IO_000727"
     CDS             15174..15701
                     /locus_tag="AC06IO_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873940.1"
                     /translation="MYNKMFKPLDTDPILYFKMYSNYTEGRVDDCCAFILMPSGLQRD
                     WVCLQSIQFAFNKCGDVLGINIIFSGNESNIHKKVRETMEGMLKLKLQYGRGEELFVF
                     DEEKKTFHLGIVPGKDTQAYLEGIIAFIKDSYRLQPDFAQDIKAQLLNKEYLAQEYSR
                     LRWKPPEKESVCVLM"
     gene            15856..17202
                     /locus_tag="AC06IO_000728"
     CDS             15856..17202
                     /locus_tag="AC06IO_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIO9873941.1"
                     /translation="MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQT
                     EKSAYLPDLHWWKQFNSKELNASIQKALQNNHQIHLAMANIESAQGQLQQVQLSWLPN
                     LTGLAGYTQFPVLGNPGTTAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVV
                     IAQTAASFFTLVAQNEALHLYNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSRIQR
                     IEAQIKVVQHNIVVTKNALHFLFNENPGDVEIKTLFENIDSNQLVPGNLPSSVLSVRP
                     DIHEAEALLKAAHADVGAITANLLPGINLGAYLGEGSNVDGAIKLGQAYLNGPIIDLP
                     LFAQVDVSKARYKAIYIKYITTIRKALRDVANDLSAYSTSSQQLNNNLSALSDEKQQC
                     HLAEVRYRHGIDDYLNLIKCQILLDEFKLVINQNKLEKLLSIVTLYQDLGGGYRGH"
     gene            17192..18229
                     /locus_tag="AC06IO_000729"
     CDS             17192..18229
                     /locus_tag="AC06IO_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIO9873942.1"
                     /translation="MGIDQQKTVRTLRLSLTCIFLFLITWYYQVPESAWTLVTIWFVM
                     YEYSTVGGVLTKSFLRFAGTALSAIYGMIVVYFCANNPLINIMALVPGLFLYAYFFMG
                     GDKTYIGTIGAVTLTIVLLNYNDIDTAVLRVFNVIIGVIGSMFMIRFFYPQYARDKVL
                     EIQLNFITQLANLLEIYLNPTQSLAAVRTKYLDYERKMLDGFTLYNRYIGEAKIETKK
                     APFFIPHSIAAMQHFRRLFRLFSVFIYYLSTEEIRSDPWVCEQLSKLMHNLQSLQRKL
                     LQQDAGPIGGDVEVAEPEEEIVIETPNIENKIATEAIVNNMQKEIALLDTEIKEIILI
                     YDVYDIRLNEM"
     gene            18286..19278
                     /locus_tag="AC06IO_000730"
     CDS             18286..19278
                     /locus_tag="AC06IO_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0257 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HIO9873943.1"
                     /translation="MSDKPKAGFPVIILLCVFLFILIYHYSYLFPFTNNAFVVANVRP
                     VAANVKGYITNIYVKNEQEVKKGQPLFTVFKAPYELAYRKAVSDVQEAKAQLLLFNKQ
                     VEKTKYLLQAQKELYEKFRFDYEHNHSALRDHAVSKLTVNTMLKEKNAALSKLKALEK
                     ELEVNQQQVIVQKKKIDSLSAVMKNAKIDLDETTVYAKQNGAVQDMFVALGTPIKTRK
                     PVFAIADTDTLFIQANFNETDLRRVQPGDKVSIFPRMYFGSKIYHGVILSRNWAASRL
                     ETHRATQIQIVRNSESNWFLLPQRLPVQIQITDYDPVHYPLSIGASAYVYIHTH"
     gene            19454..21397
                     /locus_tag="AC06IO_000731"
     CDS             19454..21397
                     /locus_tag="AC06IO_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873944.1"
                     /translation="MKIPFEGKWRATITKREAGFSQRVVVGGATSGNGSYNGVVGNSF
                     LFEDGWVELQWNNNAGSGWQESAVISSVGMTSPLVVVKFVSADDNFPNQRDGDYDDLQ
                     VRFEHLDAPFEVTQRPYALDRGSLAMFPDGIFDASQGIQYMGVRIKNTWFFDWQSNFP
                     STGMKIGIAPVSRSALSAAGIVVIDSWTPQEQEGFGQIMDGGFVRVPDLKINEETTIY
                     FKLDVSNANPSKPEIGFVAQRDAYDPRYDEPTRIVDKKIFISRSSYNPATRELVTAIP
                     EGTLYLKLNKIAIDRKGATKAAQELKKCLKQHGGGIKGMSKWLHKLSRGFWQNSKDKE
                     RWCKEQGNDALRKLLKQLLDGKDIDICHLKAILDQCCERPGRDCECCPCGDGDGRPGG
                     WDDGTGVDGWCRVKPVFWLPLDFEYKVVPNPAYIGQFGPLAFEDPWWKVVLIILAIIL
                     AIASVIYDYVYASEDDQYIIGQVVRNGDAPINLVDAAVSNLNGSRGVDLGQLDAQGND
                     INNGSPITALDSIIQLDRTAAGSYFGTLDAVLGNVVWKSGATSATTRGFISTLGTSVS
                     IPYDEHDTLSGTVTYTNEVVISELAAIPQTLSDPGDSGSVWVDLATARPVALNYGSPT
                     DSSDNRAFANPIRSVIDVLNLRF"
     gene            21416..23200
                     /locus_tag="AC06IO_000732"
     CDS             21416..23200
                     /locus_tag="AC06IO_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873945.1"
                     /translation="MKREFFQKLDKILDIASEHKQMLLQYPDVVMVGVGPERRKGKIT
                     GEPAIIVTVRKKRSSNELKKRGEKPLPQTIKGIPVDVIELGKAVEAPEIIAVQEKAKK
                     TISKIRAKWLQTPNITAIGYGYKEKGGEIDFNTIAIQFFVKKKFPMAELQNQQITPIP
                     EKINGVSTDVIQLSPIRPAAASGSRGDRHDPLKGGVAIGVGSKPFHYGTYGATVFDRT
                     TGERLFLSNQHVLDASPGTDTIQPSPVLLDDSVEVGFQLDVCLPIHFIRLDTPNTTVG
                     TVLAGAAVAAAVAAALSDEIDPTRRGQEATMPTTGSKTLFETQKVKMDYPELPIPGTP
                     FKVKTAWDYERHTDAGIKTFSVSEEKKNPHVLRDKLLITDKKLYHPGDTIRIFGLILP
                     EECAPASQSRDDRQPINPRELEDITHYPNVASSINLDLVASMDSDIASSPARRTNTRA
                     AVINPKRCRCDRYHPVAILTPMTEDRAFPVVLREPVFAQKNQIFQDLLTVVKQRDDEK
                     LLERVFVLVRYGCIYTGTLRVNSIPAGPWKHFFYVQTVNIATSDMKPLLAAQIIGGLP
                     VSQNAKADLDIACGPLTWEDGEFDIELI"
     gene            23386..23784
                     /locus_tag="AC06IO_000733"
     CDS             23386..23784
                     /locus_tag="AC06IO_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0260 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873946.1"
                     /translation="MFGFFSDYKQYITLRNFAVVYNGLTGLAVLYSLWSNPEADPSEY
                     VIDISIHALTAITLMCKQAPESVKAVAMALNTYRGFDALFKAITSLPSTIPGVANAID
                     VFNHRFNFKELEKLGNEETAETRSAVQHTM"
     gene            complement(23872..24510)
                     /locus_tag="AC06IO_000734"
     CDS             complement(23872..24510)
                     /locus_tag="AC06IO_000734"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIO9873947.1"
                     /translation="MSDVLNNYYLQTMGIEVWVMRESTPSCEKHLAALADEVASCVRC
                     SLHKTRTQTVFSRGNPKAKLMIIGEAPGFYEDQKGQPFVGKAGFLLNKMIRSIEMTEN
                     DVYIANVLKCRPPNNRDPALEEISQCSSFLTRQIKLIKPHLILALGRFAGQFLLDKPL
                     PLKQLRNHIHYYDNTPFIVSYHPAYLLRNPSDKKKAYIDWLAVKNFLVDYGQ"
     gene            24761..26035
                     /locus_tag="AC06IO_000735"
     CDS             24761..26035
                     /locus_tag="AC06IO_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9873948.1"
                     /translation="MNGQKDLKPIDVKQVIAWAFYDFANSSYFVVIFTFVFATYFTSH
                     IAPNPILGTELWGYTISLSALLIALVSPVVGAIADFSGHHKSWLFFFTYLGVISTACL
                     WFAYPDSHSIPLVLTCILISNFALEVGTVFYNSFLANLAPENYLGRISGWAWGCGYLG
                     GLLCLILSLFVFVKGEFGGLWFGTENFANIRAVTVFVALWISLFSLPLFLMVQQKTGE
                     HLTVGTAISKGLQELWLTIKSLPKQRDLFLFLIARIFYIDGLNSILALGGIYAAGNFH
                     LSVSDIMLFGIILNITAGLGAGLFAWFDDWIGSKKTILIALACLTFTFCFLLTIHSNA
                     LFWVFAPVFGIFVGPVQAASRTFLARLAKPEEMTRMYGFYSFSGKATSFLGPFLVSTT
                     TALADSQRLGMAVLIPFFIIGGGLLLLVHENE"
     gene            26156..26812
                     /locus_tag="AC06IO_000736"
     CDS             26156..26812
                     /locus_tag="AC06IO_000736"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIO9873949.1"
                     /translation="MKIIISLLFAFISASGHAFSCYDPQAIIQKPIQFDKKRIALTRE
                     YQLTHYGIDSESIEIEPKMIVLHWTCIPSLDTTFRIFDPPALPSSSPRRNELPGDLNV
                     SSHFLVDRDGTIYQLMPETWMARHVIGLNHYAIGIENIGGVDSKDDLTEEQAKANAFL
                     VCYLKEKYPQIKHVIGHNEYLQYKGTALWLEKDPNYQTDKTDPGPTFVKKVKQLIVAP
                     "
     gene            complement(26867..28408)
                     /locus_tag="AC06IO_000737"
     CDS             complement(26867..28408)
                     /locus_tag="AC06IO_000737"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIO9873950.1"
                     /translation="MTNTRDFYRLTPLTEVHWKKILINKITVRTLSQISFFIMIATIT
                     ITPFKPSHATDNKRKPTVITVSEEKFQVDGKGKKTKEFKLTFTHPDGTKDNKGYFANK
                     GDMFHVLVENKSASPITLHWHGLIVPSDQDGVPDVSQILIQPGQNKLFNYRLLQAGTY
                     WMHSHQKFQEQKQLSAPLIIYDKDDPHKNLQEVILFLEDFTYKDPQLIFDQLRTAKMD
                     MKKMDAGNDLNDVTYDAFLANKKTLAAPDVISVQASKKVRLRIINASSSTNFKIDAGK
                     LSATLIAVDGENIEPIVDSSFPIGVGNRMDLLVEIPSAGGAFPVKAMAEGTNKQTGLI
                     LKTDNTPTPILSPNTTNKTGRVNYYELEKKLKGKNALTNKKINARLHYVLDGKMNGYI
                     WTMNKQSWPDVTPKVIHFGDRVEMIYENKSSMSHPMHFHGHVFQVTEIDGQPLDGAMR
                     DTLLVQPHSTVKIQFDALNPGIWANHCHNLYHLNAGMFTTFEYQHYPKPDFYLKTIGQ
                     SKSKN"
     gene            28639..29073
                     /locus_tag="AC06IO_000738"
     CDS             28639..29073
                     /locus_tag="AC06IO_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873951.1"
                     /translation="MKRIFLTSMIGIVLNSAYAFEQMGGCGAHTWHEVTSKSGSWKIT
                     LKMISQNGQFVEPTCAEITETGLAQNNPLSYGLKFSKDADFNLAYTLTAVSEEQGFQS
                     KACVFVITANGPAQPDIQALSYHGAECTWRVIKGVGENFSVG"
     gene            complement(29088..30068)
                     /locus_tag="AC06IO_000739"
     CDS             complement(29088..30068)
                     /locus_tag="AC06IO_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIO9873952.1"
                     /translation="MHSFESIAIEFDLKNHSMQKIAIEDLNIDYQDKDKIYWIHSNLN
                     QKNIFKKLREKLRLPEQVIQLCGEKDNRSTTIEIDEALTLQIQGLCSMKLNDSYDADF
                     GNLIIHLTPNYCFTASKTLSSVLFDLLKSCPKSLPYAKTSCFLLFLLLEGVINDYAKI
                     HLAYEELADQFDAQVRTTNKNTYSQVLELKHGVMKIKRYTIAIREILMRLTSRNILVI
                     SEQCRTSLYNLSNHCHLIVNEVDSLRDMLNGLLGQIDNQLMQNMNETMKVLTAFAAIF
                     LPLSLITGIYGMNFYWMPELGWKYGYFWALGLLVLCALLLFLIFRKKKWF"
     gene            30315..30911
                     /locus_tag="AC06IO_000740"
     CDS             30315..30911
                     /locus_tag="AC06IO_000740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIO9873953.1"
                     /translation="MNQDMEKIEQMYQLIMEMNKLKLVFRNTITNPQRKESTAEHSWS
                     ASMITLILMNELKREFIDVDELKTMKLVLIHDVVEIYAGDVLAFDVEARKDKEKVELE
                     ALEKLSAVYPSFGIELDSLWHEFEERKSLEAKIAKAADAICPIFQRLQAKQSYIPFNI
                     SMAQLEKTKYPYFQFSKTFSNLFEKLKADLLKEQLIFL"
     gene            31046..32545
                     /locus_tag="AC06IO_000741"
     CDS             31046..32545
                     /locus_tag="AC06IO_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIO9873954.1"
                     /translation="MYDDLWKPYASIELPSLKLGGIVLNGQLLSETPIHLVLKAFNRH
                     GLIAGATGTGKTKTMQVLAEQLSLNGVPSLVMDIKGDISGLAMPGDASTKLLERSQLL
                     HIDFTPRGFPVELLTLTPQDPGVPLRARIDDFGPLLFSLMLDLNDTQAGVVTVLFEYA
                     KANQLNLVNLDDFKSLLQFAQTDRGNQLIAAQYGGVASTSIGTIVRKIIELESQGGKQ
                     FFGEPSFVIQDLLRTDANGYGVISILRLLNMQDKPKLFSSFMLKLLSDVYRQMPELGD
                     PDKPRLVLFIDEAHLIFNYSSKALLNLIETIVKLIRSKGIGLIFCTQTPNDIPEAVLS
                     QLGLKIQHSLRAFTAKDRKALKLVSQNFPVSSYYNTEQLLTTLSIGEALVSALDAKGQ
                     PTPLVHAMIRAPESRMGPLSNEELKVLLDQSLLFGKYSQMIDAPSAKEKLAELISEQQ
                     VAKQQTSQKENNSSVLNSLSKNTLFRQVVRQVFRELTRAILSLFKSKRN"
     gene            32626..34029
                     /locus_tag="AC06IO_000742"
     CDS             32626..34029
                     /locus_tag="AC06IO_000742"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIO9873955.1"
                     /translation="MINITGTYTDQYQLAMAQVCFLKGQHERIATFDYFFRALPFKGG
                     YAIFAGLEDLLDVLEQLRFEQQDIEFLQHQNMHPDFIRYLQQFKFNGTVYTSLEGDVV
                     FPTRPVVIIEANLIEAQIIETLLLNILNFQTLIATKARRIRQVAGERILIDFGLRRAQ
                     GAGGYFASRAAIVGGFDSTSNVRAGRDYSIPVSGTMAHSFIQSYDSEIQAFSDYAEAW
                     PENCILLVDTYNTLQSGVPNAIKIAKEMEKRGHRLKGIRLDSGDLAWLAKRARHMLDE
                     AGLNYVAITASNQLDEYVIKNLLEQDAPIDAFGVGTNLAIGAPDAALDGVYKLAFING
                     QPRIKLSDTPAKITIPYQKQVYRVFERDGTYRGADVVCLKTEKKIDMMHHPFEILKSL
                     SIKHYKKEALLHKVMENGKRLLPKKSLGEIARFSQQCFDRLPLEYKRFINPHIYKVGL
                     STELKMKRDQLIAEHSR"
     gene            34034..34654
                     /gene="pncA"
                     /locus_tag="AC06IO_000743"
     CDS             34034..34654
                     /gene="pncA"
                     /locus_tag="AC06IO_000743"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HIO9873956.1"
                     /translation="MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVAS
                     QDWHPSNHISFASNHTGRKPFEKIKLGNLEQTLWPDHCVQGSIGAQFHAQLNTNPIEA
                     IFRKGTDPNIDSYSGFYDNLHQKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
                     RQGFDCYLIEDATQALVKDDFIKIKKELIKQGSHTINSSNLLNNLQ"
     gene            complement(34737..35120)
                     /locus_tag="AC06IO_000744"
     CDS             complement(34737..35120)
                     /locus_tag="AC06IO_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIO9873957.1"
                     /translation="MSIQLNHTIVWVKDRDKSAHFLTEILGLPEAIPYGPFMVVEMSN
                     HVSLDFCKTDKEITPQHYAFLVTETEFDDIFKRICGRKMDYWADPGQTRLGQMNYNDG
                     GRGLYFNDPNGHLLEIITKPYGSGS"
     gene            complement(35120..36334)
                     /locus_tag="AC06IO_000745"
     CDS             complement(35120..36334)
                     /locus_tag="AC06IO_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9873958.1"
                     /translation="MYPKTKLSFVTLLLLISFASVNAVLFTPALPSITAFFGITEHAA
                     QQTITSFLVGYAIGQLIYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLV
                     LGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
                     YGWESCFYAGTVYGAILLAMVRQLPETQKNLQLEAFQMKHLLQGYVSQFKNKPLISGG
                     ALMGGASCFVYIFAATAPFIAINLLGMSTTEYGAANILPSLGLTLGSIWSAQFAKKYS
                     QRAGIQLGILISGFAALAMLMAALTHHSALIKLFFPMIFIYLGLSFIFANASTFAMSH
                     TSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCILMMWLFFSFIKSN
                     EIISSIDRNKES"
     gene            36452..37315
                     /locus_tag="AC06IO_000746"
     CDS             36452..37315
                     /locus_tag="AC06IO_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIO9873959.1"
                     /translation="MGKFDQITYFIAVVEEHGFAAAARKFGVSTAAVSRQIARLEAEL
                     KAELLVRTTRKLSLTDIGARYYQQCKKTLLELNEAEIAIASSRNEAVGILNVTSSRYF
                     AMRFLLPYLPEFMELNPKLQIKIELAERFPDLAQENVDVLFGVSMEGPPELVRKRVST
                     TRYVLCASPDYLAKNGIPKSPSELGKHRYITHSMRNPDNLVTFKDNEQIYVEPVLWLN
                     DSRALCECALLGMGIIKLHEYIVAEALQDGRLIEVLPEFQESSLSVYLYYQASRYLQP
                     KIRRFIDFFTK"
BASE COUNT        11162 a         6867 c         7853 g        11610 t
ORIGIN      
        1 cattttgttt cctgaattct ttagcgaaca aaaaatctgg tagtcagagt aggaatggac
       61 ttctgtctgc aggaccaaga cttgttcgct tgatgcataa aaccaaggca aaaatatttt
      121 ctcaataccg ttacttgagc taagctttca ttcttgcaaa ataaaatatt tgtcttaatg
      181 tgctaaattt accataatag cccctttttt aatccaatag tactatgtca tcaaaattgc
      241 ttaccatcca attgttagaa gagcttgtgc atgctgcaga attaaatcaa gaaggcaaaa
      301 ccgcggatta tatccctgaa ctggcgaatg ttaatcagga attgacggca attgcggtgc
      361 aagctttggg tgagaaaacc ttggcttata gtaataatcc acttcatcca gtgactttgc
      421 aaagcacggg gaagatgatc cctttgattg gtttgcttga ggagtttggt gctgatcaat
      481 tgtttgaatg ggttaaagta gagccttcgg gcgatgattt tgcttccatt actcgcctgg
      541 agcagtttgg acctaaaccg tcaaatccca tgctgaacgc tggcgcaatt gccttatgct
      601 cacgtattcc aggcgtgggt gagcagcagt ttagatggtt agaacattgg gtacaaaaat
      661 tatttaatca acgtctcagt attaatcctc tggtttttgc ctctgaaaaa cgaaccggta
      721 atcgtaaccg cgctctcgcc tatttactga aaagcagaaa caatcttgga gctgatgttc
      781 atgaaaccct ggatttgtat tttgcattat gctcctatga agccatgttg gatcagatgc
      841 tgtacttacc tactttgtta gctaatagcg ggaaagattc ggatacgggt gaacaaatac
      901 tgtctatgga aacctgtaaa attactttag ccattatggc gacttgcggg ttatatgatg
      961 aaacaggtac tcacatggtt aaaacaggga tgccagctaa aagtggagtg tctggttata
     1021 ccatcgcggt tgttcccggt aaagcaggta ttgtggtgtt gagtccaaga gtgaatgcga
     1081 aaggaaatag catacgaggc gaaatcatgc ttgaaggttt atccaaagca atgaattggc
     1141 atttcgcatt accttaattt ttaaaaaaga gcgttataga atagtgagct atttctttga
     1201 ttgaagtgaa ataacgatga caaattgctt gtcacagcgg agtggaaaaa cttaattaca
     1261 ttttgattaa aggattttac atgaaaattg ccgttattga gcctataggg attgcaatgc
     1321 aggaaattcg ttttgaatta ccaggccaca cgattatcga atgtgacagt cgtcagtgga
     1381 gtgatgagca attaatagat tttgtgaagg atgcagacat cattgcacta acaaatcgac
     1441 gtttatcggc tgcagtcatt aattcagcgc tgcgattaca attgattgca gttgcatttg
     1501 ctggaatcga ccatattgat aaggatgcgg tgagtaaaag aaatattccg gtaaaaaatg
     1561 cagcgggtta tgccaacaca gcggtatctg aactggtatt tggtctcatg atttctcttg
     1621 cacgtcatat tcctgataac aatcagagaa taagagagca tggcataact aataccggta
     1681 ttgaactaaa aaataaagtg ctaggcattg tcggttatgg agcaatcggc gcagaagttg
     1741 cgcggcttgc taaggcattt caaatgacaa ctataattta tgacaggaaa tctcaagtaa
     1801 cattagacga tttgttttcc caatctgatt tcgtcacttt acatgtgcca cttacaaatg
     1861 aaaccaaagg catggttaac ttaagatttt tatctcaaat gaaaaaatca gcgtatttga
     1921 ttaattgtgc cagagggccg attgtggtta gcagtgattt aaaaaaagcc ttggaacagg
     1981 gcatgatagc aggtgccgca ttggatgtat ttgatgttga accaccatta cctgcaaatt
     2041 atagcctttg ggaggcacct aatcttatcg caacaccaca cataggattt aatacaagtg
     2101 aggcccttgt ggccaagggg caattgacta tcaaaaatat caaagagttt ctttcgtccc
     2161 gcttataaaa ccgtttaaaa acataaagaa acataagaag ttgattgcaa aatagcttgt
     2221 agtacttcag atgtagttta acccagggca aaatcatgct ttaaccatga gttatggata
     2281 aggattatat aaaaaccagt caatatccag gaaccgtttg ggctgaggag gcattgatgc
     2341 cgtctcgaag ccttgaacgg ttcgaaataa caaccgatct tatccataac tcactttact
     2401 ttacctgcaa taatatataa aaccacgctt gctgtttttt aaaataattt taatgttatc
     2461 ctacgcaata cacttgacca gtttgagcgc cctcaatact cttgctgtag gcgagtgctg
     2521 cacgagcagc ggatacaggt tcatagcctc tgaaataggg accatagttt tccatagctt
     2581 cggtaattac tgtggggctt acaccattga ttcggatacc tcttggcatt tccagagcag
     2641 cacttttgat aaaaccatcc aatgcgccat taaccatggc agcggaactt ccatagagaa
     2701 tgggatcacg atttaatatt ccgcttgtta atgtaaatga accattatca ttgatgtatt
     2761 ttaatccagc caagactaca ttaacttgac ccattagttt attggtcaaa ccaattttat
     2821 aatcgtcttc tcccatgtcc tttagcgcag caaagtgcac tttaccggta gctaacacaa
     2881 ccgcatcaat agtatttaat tttttgtaca ttgcatcgat tgattgttta ctggttatat
     2941 ccacttgaat atcactgttt ttatagctcg ctgaaataat ttcatgtctt gggtttaatt
     3001 cagcaactat tgcttttcca attatccccg tagcaccaat caccactatt ttcataattc
     3061 attccctctt tatccaataa aaagcaaaag tcctacatag aatagtacag taacaacaat
     3121 gagttaaata caggacttac gaaaaacccc aatctccatg ttaaaaaatg tttttaattc
     3181 agtcatttag tttatctaac tccctcatta aaaacatttt ttgccttgac catggagttt
     3241 ttcgtcagtc ctgaaataaa aagtgtgtat cggctgtatt tgattactgc ctatacacag
     3301 gatttattga agaagtgaag taaaatgcct ttattaacca cttgccttgt agcatgtaat
     3361 atggcgagta tggtttacat caattcaaat tatttgcgca aaccacagat tgatgccttc
     3421 caccagagta gggtgagcga acatcatatc gcgcaatttt tgataaggta cgcgcaactc
     3481 cattgctaat tggatgcccc ctaatatttc ccctgcctcc gcacagaaaa tagaaacgcc
     3541 caaaatcaaa tcggtttcag cgtcaatgac tgcttttaaa acacccgttg tttctccttg
     3601 ggttttggcg cgaggaatcg ccgctgccgg aattgtggcg attttaattg ggcggccttg
     3661 ggatctggct tgagcttcag taagtccaat gcgtgctaat tccgggtcga gaaacactgt
     3721 gtaaggaata agcctgtttt gggaagaaag ctttttctgg gggttttgta aattatgttt
     3781 caccagacga tagtcatcaa gagacaggtg ggtaaactgt gcccctccct tgacatcgcc
     3841 caatgcccaa atccctgccg ctgtcgtttc gagaaactca ttgactttga tgaatccacg
     3901 ctcatcaagt tcaacgccag ttctatccaa atgcaatcct gctgtattgg caatacgccc
     3961 tactgcaaca aggacagctg tgccacgaat aatttctgat tggccttgtc gattagcttc
     4021 gataatcact tcggtttgct cttggcgaat ggcattaatc tttgtatcga cagcaaattg
     4081 aatgccttca ttggataagg tttgaaaaac ctgttcagca atgtctttgt cttcccggcc
     4141 aagaaattcg ctactggctt cgatgacggt gacttcagcg ccaaaccggc gaaacatttg
     4201 tgcaaattcc aagccaatat aaccgccgcc gatgatcaat aaatgttggg gtaccgaatc
     4261 ggtgttcatt aaactgtcat tggtaaaata attgacttta tcaagccccg caatgggggg
     4321 agtgtaaggc agggcgcctg tattaataat aatcttatca gctgtgatgt gcagcgtttt
     4381 ttgattgcca cgcggtgagg atagggtaac ttcaatcatt tttggtccaa taaaatgacc
     4441 atgtcctaac atgagatcca tgcccgaatc taaaaattgt tttaaattcg cttcacgcat
     4501 cccattcacc acagcgtctt tacgtgctcg aattgcttta aaatcaattg aactcaatgt
     4561 ggtatttaaa ccatagtctt ttgctttacg gcagtaatgt gccacttttg ctgactgcac
     4621 gagcgttttc gtaggtatgc atgccacatt gatacaggtc ccaccgatct gattgttttc
     4681 aaccatcgcg attttctggc cgcttttagc caaatccatt gcaagagtct tcccaccttt
     4741 gcccccacct aatatgatgg tatcaaactc taaaaccatt ccattctccc gaaaatttca
     4801 atgttttatc cacttgatga tgatccttat ttacaatact tgaattaaag aggaaagctc
     4861 acgcaaggcg ataaaaaata tggtgtaatc tatgttttcg ctacccaata acatttctaa
     4921 taacttttcc cagcgttttt gaatggggtg attttgttcc agccaatagt ttatcaattt
     4981 ttcagaagta cgttctttgg tgttagttgt taaaatggca atagtcaaac gtctttgaag
     5041 attatctaac tcatcacgca aagacaggcg agccatggta ttccaatgcc cttcacgtga
     5101 gtcattggct atttggtcgc gaaaccaaac gagattaaat tgactgccaa cgttaaagta
     5161 agtttctgca gtaagcgata aatcaaattc attttgcgta gctacttcaa tgatatttaa
     5221 tgaagtataa agagcccgag ttatagcaat tttttgagcc atatcttttt caatgccaat
     5281 actgacgaat tggctaatga gtttctcaag gtattcttta gtgacaccac ccatgagctt
     5341 gggaatcaac tgttcaagtt ttttgactga cttgccataa tttttaatat ttttttcaat
     5401 acctccggtc agtcggttat tatgcaaaaa ccagcgtgtc gccaagttga gcaattggcg
     5461 aatatgatgc aataattcat attgggtgga taaggatatt ttatcttgaa atgaatcaat
     5521 cagattttgt aagacatccg tttggaaaat acaggctgaa atagtgtaag cgcaggcaat
     5581 atcagcaatc gacataccgg tttccatcag catgcggtac atgaaggtaa ttcccatgtt
     5641 gttaaccagc ttattgctca gttgcgtcgc tatgatttca cgagacaaag ggtgttttgg
     5701 caaggctttc gcatagggct ttcttagcga gatgggaaaa cctgtttcca aaaaagttgt
     5761 aaagtatgag tcatcgggta aatgtgattt taataattcg ttagcaatat gaattttggt
     5821 gtgtgccatt aaaacagcaa gctctggtct tgtcagtcct gcgccagtgg ttttgcgttc
     5881 gaggagtttt ttgtcgtcag gaaggaattc aacgcttctg tcaaggttaa cccacttttc
     5941 caattctttc atgtaatctt gatataagcc actataatga gaagaatgag ctgccgatat
     6001 acttaaaatc agggcttgat tgtagttgtc ttgcaaaaca agatcggcga cttcgtctgt
     6061 cattctgctt agcaattgat tgcgtttttt ttctgtgagt ttcttttctc taatctcttt
     6121 atctaaaaga attttgatat tgacttcatg gtcagagcag ttaacgccgg cggaattatc
     6181 aatcgcatca gtgttaataa gaccgccctt taatgcaaac tcaactcgtc caagttgagt
     6241 aaaacccaga tttcctcctt ctcccactac cttgcagtgt aattctgcgc cattaatacg
     6301 gcagaattca ttagtcttgt cgccaacatc ggcatgtgac tccgtggttg ctttgacata
     6361 ggttcctata ccgccattaa atagtaaatc cactggtgct tttaacagcg ctcgaataag
     6421 ctcgttgggc gtaagggcat ttgcttctat agcgagagct ttttttactt gcgggctaat
     6481 ggggatcgat ttgctcgagc gtttgtaaac gcctccgcct ttagaaataa gttgtaagtt
     6541 gtaatcttcc caggatgaag taggtaaatt aaacaggcga agtctttctt cataggattt
     6601 tacagggtcg ggattcgggt cgagaaaaat atgacgatga tcaaaagcag ctaacagtcg
     6661 tatatgcttt gaataaagca tgccattgcc aaatacatca ccactcatat caccaatacc
     6721 tactaccgta aagtcttgtt gctgaatatt gatatcaagt tcacgaaaat gtcttttgac
     6781 agattcccac gcaccacgtg ctgtaatacc cattttttta tgatcgtatc cggcagaacc
     6841 tcctgacgca aaagcatcac ctagccaaaa tccatgttcc ttggcaatac tattggcaat
     6901 atcggaaaaa gtagctgttc ccttgtcagc agcaacaacc agataaggat cagggtcgtc
     6961 gtaacatata acattctgtg gagggattgt tttatcattg attaaattat cggtgagatc
     7021 cagtaaacca ttgatgaatg atttataaca agagatgact tcctgttgga tttgttcgcg
     7081 gctggctgca tgaagcggtt ttttcaagac aaaacctccc ttggcaccag agggaacaat
     7141 gactgcgttt tttaccttct gcgctttcat aagtcctaaa atctcggttc gaaaatcttc
     7201 tggccgatcg gaccagcgaa tgccgcctcg ggctactttg gtactacgta aatggattcc
     7261 ttcaaaacgt gttgagtaca caaaaatttc ataaagcggt tgacctggag gtaagtcagg
     7321 aatttccgag gattttaatt tgaaggaaag gtaatcttta ggtaaccccg cgtgattttt
     7381 ttgaaagtaa ttagtacgaa gagttgcttt catcaaagac caaaaataac gcatgatgcg
     7441 atcctcatcc aggctgctaa ttgcatcaat gtttatttga attttctgtt ctagtgccga
     7501 catgtctttt tgaatagcag atttcctttt aaggccaaat ttcaaataaa aaaagtgaat
     7561 cagatcacgg gcaatggctg catgcatttc gatggttttt tcaatgtaag attgactgta
     7621 acgaatgcca atctgatgca ggtattttgc ataggctcgc aaaatagtaa tttcttgcca
     7681 ggagagtccc gcggataaca ccaatttatt aaaaccatcg ttttcacaaa taccaagatt
     7741 gatattaatc agtgcctcgg caaaaatatt gttgacctgg gtaatattga atgaatcagt
     7801 ttgagaatag gttacgttaa aatcactaat ccagaataat tgcccgtccc gtgttttgat
     7861 tttataagga cgttcggtat aagtgcgcag tcccatgttt tcaagcatag gcaagatgtc
     7921 cgataggggg attggggtct cataacgata aagtttaatg tgtaaaggat attgtaggtg
     7981 agttttttca taaaaatcga taaccagagg attatctttg gacagttttt ctatctgtaa
     8041 aatgtcctgg attgcctctt cgggagtatg ttgttcacaa taactcattg acattgtttc
     8101 ggtgtattta tcaatgagct gtttcccttt tggggagcca aattgctgag ttaattgctt
     8161 ttgcaattta tctttccatg agtttgtcat cattcttaaa attccatgca agatatgttt
     8221 ttatcgttac tcaaaagtat aggccatttc aaagaataaa attaaaattt gctaacttat
     8281 ctcatacaaa gtgaatcatt tgttttcagg agattattaa gtaagcttta ataaaatgtt
     8341 aaggacataa aagtataata aagcttcttt caaaagacga agcacctatt atgtataaaa
     8401 atatactgtt tttttcaaat tttagccgta tgcgttggaa cgagtacgat ggggatcgat
     8461 atcaaagtct ttgggaacat aataagcgag atattaaaca cacatttttc ggcacgattg
     8521 caaacgggaa agatttgaga gaagtgctat tccagccagt tactgcttct ttttcgatgc
     8581 tagtcgcggg ggcatttgcc caattattgg tgccctattc cattttcatt ctttgcatgg
     8641 gcgaggcggg gtttgcgggt aaaacattaa ttaattattt ggagataact acggttgctt
     8701 tggcaatgcc gtttattgct ttattaagta ctgtaggccg atgtattgca acggttaaat
     8761 gcctggtgga agatcaatac aacctggccg agatggttga agacaaagag gttaagccaa
     8821 ataaaatcta tcttgattgt gctgaagtaa actacactgt cagggatgag aacaataacc
     8881 agttcagtcg caaatattat aaattccaat ataaaatgag aggtctcaac gatgagcttt
     8941 gccagggatt ctttactttc agagatttag agcgtgctcc tgatgatgac gatttcaatg
     9001 gaaagctttc cagtctcaca ctttataaat acgacatcct ggatttgatt aaaagaattc
     9061 ataaagataa atttgaagaa atggaagacg aaaatctaat tcgtatatcc atttaattta
     9121 gattggttag aaaatcaggc tgtttttggc tggaagctgt aatggagagt gattattttt
     9181 ctcaactact tgccacttaa gaggccacgt tatatcgttt atgaatgata tgattggttc
     9241 ctgcctatat tcttgatgcg ctgacttggt aatgagttat tgagttggcg tctaaagcga
     9301 gacaaaacat agggatgaaa taaataaaag gattaaggga ttaagatcgt gcgtttatct
     9361 tatggtttat taggtttcat gatatttaat ttggtggctt gtgaaaaaat ggcattactg
     9421 actacaccac ctaaaaaaca gcaattatct aatagtccac tggctactcg ggcagagcga
     9481 tatttttggc gaacccttca tgaggggcga tatcaggaca ttccccaagc ggattatttg
     9541 ctgatggcag cttaccttga gaatccgtat gattcgaaat tagcggccca tcttggcttt
     9601 attcatattt ggaaaattac ggagcgggaa agagtaaaaa ataactctcc cttgcttccc
     9661 aatgaaatta ttttatctaa aaaatacttt gctgatgctc tgcaactgga tccggaaaac
     9721 ccaatttatc aaggatttta tggcgatacc caattagtgg aagggcagat ttttaaagac
     9781 aaacaagaag aagtcagggg atactttact ttacagaaag cgataagcaa ttggcctgaa
     9841 tttaattatt ttactgcagg gtaccccatg agtagtttaa gtgccgattc ggatcatttt
     9901 aaaaagggtt tggattggca atggaaaact ttggatatct gtgctggtga gaagattagt
     9961 agacagaatc ctgattttac gccttatatg aagagggaaa caacaacagg cgcgcaaaga
    10021 gcctgttgga attcagaaat cgcgcctcat aattttgaag gtttttttat gaatatgggg
    10081 gatatgctag ttaaatccgg agatcccgaa acgggaataa aaatatacaa caatgccaaa
    10141 ttgactaaaa attacaataa atggccgtat aaagacatgt tagagaaacg tattctgaat
    10201 gcgaaagcca atgtgcaaca ttttaatcaa aaatccaatg accccaatca gtcgattatg
    10261 tttaactcag gatatggatg cgtggtttgt catcaacggt aagtaccatt tgattattcc
    10321 atattaggtg acttgttaga ttagtaagaa tgtgaggttt cagtttaaaa cccctgcttg
    10381 tgtttttgca aagcgagcag gataaaagcg aaagaaagtg ttatagtttt aatttatttc
    10441 ggcaaagaat ttaaaaagaa catggaaaaa attactattt atcataatcc aagatgctca
    10501 aaatctagac aaacattgga aatactgcaa aacaaaggtt ttgaacccat tatcattgaa
    10561 tatcttaaaa ctcctcttga tctggagcaa ttgaaaagat tacggactca cctggctctt
    10621 gaagatttgg tgcgcacgaa tgaaaatgtt tttaaagaat tgggcctgtc tctggctaat
    10681 gaggcgcaag ttttagaagc tatggtaata gaacctatat taatgcaacg cccaattgtc
    10741 acatttaaag gtaaggctgt tatcgggcgc ccgcctgaga aagtcctgga gctgtttgat
    10801 tagtctttat tgtacgataa tataaattta atcctgatac catgactaat ctcactgtca
    10861 tggtaaggaa taaaagcatt tgcaaaactt cagagagaat agctttttaa ttgtgatgca
    10921 aattgcctta acacaagcag tccggattct tcggcctggc tacaccattt ttgtattaaa
    10981 ttaagaagat ccttattgga tagggacgtt tttaaccata tctgctgtaa taattcgcgg
    11041 taattatgaa ccgcttgcaa gttgtcatgt tgcttaagta atgttttcaa tcgcaactga
    11101 tcagcatggg atagctgttc attcggtttc ttgagtaaat ggcgagcatt ctttaaatcc
    11161 cgatttttta tgcggtattg catatcgcgc agagtaggaa atataaccag cttgtaataa
    11221 tcagatatca cttgaaagcg attgccaagt atagctttta ccgaatctac atctatatga
    11281 gatttattct tgtcaatgat cagttttggg ggtaattttt ttactttggc aagctttaaa
    11341 aaagacaaac aacgtatgta aaaccaaccc aaatcaaatt cccaccattt aatggaaaat
    11401 ttggctgagg aggcgaaggt atgatgatta ttatgcaatt cttcaccagc aatcaataaa
    11461 ccccagggaa aaacattggt tgaagaatct gacgattcat gattgcgata accccaataa
    11521 tgccctattc cattgaccac gccagcaaca agaggtatcc acatcatttg gattgcccaa
    11581 agggtaagtc caggaattcc aaacaagaaa agattaagtg cgagcattaa aaatatgcct
    11641 ttgttcgcat ggggagagta taatttgcgc tctatccagt cgttgggggt accataggag
    11701 tactttttaa tcatgtcttt atctttagag gcttctcgat ataactctgg aacctgccaa
    11761 aatacttttc ttatattaaa tacctgaggg ctatgcggat ccccttccat gtcagtattg
    11821 gcatgatgtt tcctatgaat ggctacccat tctgctgtgg tcatccctgt cgttaaccaa
    11881 agccagaaac ggaaaaaatg gctgacaaga ggatgcagag ttaatgcgcg gtgtgtctgg
    11941 tagcgatgca ggtaaatggt aacagcagtg gtggtgatgt gagtcaggac aaaaccggtc
    12001 aaaagatatc cccataaagt catatttaaa aaaccaaaca tcattcctcc tcttaggtgc
    12061 ctaaaattgt gtaattggca aaacaaaatg taagaatatc tttatatcca ctaagtatat
    12121 actatttatt tttatcttag tggcatttaa aaagctaatt atttacgcgc ttttattaaa
    12181 agccgtcctg gattttctgc tgatattgcc cttatattct gcttttggcg gcgcaaattt
    12241 ttgaattttg gctgaaacta aatctgaaaa gttatgcaaa aactcaaaat ccttaatttt
    12301 aatgcgctcc aatttttgat tgattaattt ttctatagac agcagcagct tgatttcttc
    12361 tgtgctgacc aaggatacag ctaatccaga ggcgcctgcc cgtcctgttc gtccaatccg
    12421 atgaacgtag tcctcaggca catgaggcag gtcaaaattc actacacagg caagtttctc
    12481 gatgtctatg ccacgtgctg cgatgtctgt tgcaattaat gtttgtactt tgcctgactt
    12541 gaaatcagcc aaggcttttg ttcgttgagc ctgtgatttg ttgccatgaa tagcaaccga
    12601 atatatttgg gattcggcca gttgcttaac cagtttattg gctccatgtt tggttcgaga
    12661 aaaaaccaaa gtttgtcccc agttattctt atgaagtaaa tgactcagaa gagctaactt
    12721 atggtttcta tctactgaat aaacagtttg ttttatggtt tttaccgcgg tatttctcgg
    12781 tgcgatatca atttccaccg ctttattcag tatatttctg gctaaagcgc gtacttcagg
    12841 ggtaaaggtt gctgaaaaaa gcaggttctg cctttttaaa ggtaataatt tgataatttt
    12901 ttttatatca tgaataaacc ccatatccag cattctatcc gcctcatcga gtaccagggt
    12961 gtcaatctca tcaaatttaa cagcacgctg ctgatataaa tccaataatc ttcccggtgt
    13021 tgcaaccagt atctcaaggc ctgagtctaa ttgtttaatt tggttgtggc tttttactcc
    13081 tccgtaaatc acagctgagc gcagtgttag gtatttgcca tattggatta tgctctcgtg
    13141 gacctggatg gctaattcac gtgtgggcgt taaaatcagt actttaactc gattagggct
    13201 ggctggagtt tgctgaccgg ctttttgcaa gataggcaat acaaagcttg ctgtttttcc
    13261 tgttcctgtt tgagcagaac caagtaaatc atgcccattt aaaatgagcg ggatggcttt
    13321 taattgaata ctggtagggg tagtgtagcc taactcacta accgccctgt ttagcggttc
    13381 aatcagcgct aattgtttaa aactcattgt tttccttatt gatatctatg ggtattgtgg
    13441 gctctgaggt taatcactca ttattttgtg gggaaaatga ggcattaatt agaggggaaa
    13501 aaaataggaa ttttcaaaga ttttttgaaa gaattcatgc aatatttgga actattataa
    13561 cagttatttg taaatatgtc ttgtttattt tgcttaggtt aagaagagag ccatttattt
    13621 tcttgtgttt tatctatccc atatgcaaaa ataacaacca tagtatttgg tgataatagt
    13681 cagcatgaaa gcgaaaggtt tttttgatga gcaatcttcc cttaagaaaa gtaaaattgg
    13741 gttgaattta gtatggatgt tctcactggg tcatattttt ctaataacgc tcagtaatgt
    13801 tctggttcaa tatccatttg atatgatggg atttcacacg acctgggggg cgttcactta
    13861 tccagcaatt tttattttaa ctgatttgac aacaaggata gcaaccgcta atgaggcgcg
    13921 gaaaattatt atgctgagta tgactccagg attaatattg tcctatcttg ttgcgagcta
    13981 tattgaggca ggcaataatt tgaatgagag cggtctatcc attttacacc tcatgccatt
    14041 acgtatcgcc ctggcttgtt ttatagccta cgttgttggg caattgctgg atgtggcagt
    14101 tttccaacgc tatagaaatc ggcctgcctg gtggctggct ccaactctat cggcggttgc
    14161 aggcaatttc atagatacgg cattattttt taccatcgcg ttttatcact gctccaatcc
    14221 atttttaagt cagaattggc ctgaaattgc gatggtcgat gtctttttta aaatcatgat
    14281 tagtttaatt gcttttgttc cggtttatgg tctggtttta agtattcttg gtataaaaat
    14341 taccaataaa ttaatggtat aaaaggtatc ttgaaaccat tttcaaaata aggattgatg
    14401 atgctgactg gtttaaggtt gcttacaaaa gacctcaaga ttttaaatgg caaatacatt
    14461 cagctagaac ccattaccag ccagcaccgt gaggagttac gaaaagcagc aaatcatgaa
    14521 caaatttggc aatacatgcc acacaaggca acgggcaagt tatttaattc ctggtttgat
    14581 gattgtcttg agaaaatgtc cataagagac caaattactt atgcagtgcg ttgtaaaaaa
    14641 gaaggggatc tgaaaggagc tacttcattt tacgatattc aaccagaaaa caaacggctg
    14701 gctttaggct atagttggta cattcctaaa gtatggggaa caacgacaaa cccggaatca
    14761 aaattactca tgttgcatca ggcgtttgat gcttggggga ttaatcgagt agaactgggt
    14821 acagattctc gcaatattca ttcctatcgt gctatcaaaa agctgggtgc gacggaggaa
    14881 ggggtgttgc gccaacatat gattcttcat aatcaggtga taacggatac tattatgttt
    14941 agtattcttt tctcggagtg gccggcggta aaagagcgat taatacagag attgaactat
    15001 gctgcaaccg ataaagacca cttggccgcc tctatatagt gatcccttgt tctctgggtt
    15061 aaacagctca actcatgaaa taatcagaca cacaataaat agaatgaaaa aagattggta
    15121 tttttcttta attgagtgaa tatttggttc tacctgcgag aaaggagtgc tcaatgtaca
    15181 acaaaatgtt taagcctctg gatactgatc cgattttata tttcaaaatg tatagtaatt
    15241 atactgaggg aagggttgat gattgttgtg catttatttt aatgcccagt gggttacaaa
    15301 gagattgggt ctgtttgcaa tcgattcaat ttgcttttaa taagtgtggt gacgtccttg
    15361 ggattaacat tattttttct ggaaatgagt cgaatatcca caaaaaggtt cgagaaacga
    15421 tggaagggat gctcaagctg aagcttcaat atggaagagg ggaagagctt ttcgtttttg
    15481 atgaagaaaa gaaaacattt catctgggga tagtgcctgg taaggataca caggcgtatc
    15541 tggagggcat tatcgctttt attaaggata gttatcgctt gcaaccggac tttgcccaag
    15601 acatcaaggc acaacttctt aataaggaat atctggcaca ggaatatagt cgtttgcgat
    15661 ggaagccacc agagaaagaa tctgtttgtg tgctcatgta ggttgtagaa gcattgtttc
    15721 cactttaaaa atagaaaaat ttgaatttta tggggattat tgcattccat taacttggtt
    15781 tgcgtaatac tctcttgaca actacaagat tgaaagctca atattcttta tttacaccaa
    15841 ggttcaggat tttcattgta taggaagcga tggctcattt tattgtgtgc tttgttgaat
    15901 aatggttgcc tctggcttgg gtcagaatac aggaaacctc atgtcaacac cccgcaaaaa
    15961 tggccgcata aatattcaat tcaaactgag aaatctgctt atttacctga tctgcattgg
    16021 tggaaacagt tcaatagcaa ggagctcaat gcttctatcc aaaaggcatt acaaaataat
    16081 caccagattc accttgctat ggcaaatatt gaatccgcac aggggcaatt gcagcaagtc
    16141 cagttaagtt ggttgcctaa tctcacaggg cttgcaggct acacccagtt tcctgtttta
    16201 ggtaatccgg ggactacggc aattgcctat ccagcctaca tcattaatat tttccagcaa
    16261 tataagcaac aaaaaagcgc acaagccatg cttgaagcca gtatttacgc gcaatattct
    16321 gccagactgg tcgtgatagc ccagactgca gccagctttt ttaccttggt tgctcagaat
    16381 gaagctttgc atttatacaa taaactgctt aaggactatc gaacctatct caaactgact
    16441 caaagccaat atcgctcggg tttaatttcg cttgacaaca tcactcaaat caaaagtcgt
    16501 atccaacgaa ttgaagcaca aatcaaggtg gttcaacata acattgtggt gactaaaaac
    16561 gccttacatt ttttatttaa tgagaatcct ggagatgtcg agattaaaac tctctttgaa
    16621 aatatcgata gtaatcaatt ggtaccaggg aatttaccgt ccagcgttct cagtgtgaga
    16681 cctgacattc atgaggcaga agcgttgtta aaagcagcgc atgctgatgt tggcgcgata
    16741 accgcgaatc ttttaccagg gattaatttg ggtgcttacc tgggtgaggg ctcgaatgtg
    16801 gatggcgcca ttaaattagg gcaagcctat ttaaacggtc cgataattga tttgcctcta
    16861 tttgctcagg ttgatgtgag taaagctcga tataaggcaa tctatatcaa atacattacc
    16921 actatccgta aggccttgcg cgatgttgct aatgacttgt ctgcctattc aacttccagc
    16981 cagcaattaa ataataattt atcggcttta agcgatgaaa aacagcaatg ccatttggca
    17041 gaagttcgtt atcgtcacgg aattgatgac tatttaaatt taataaaatg ccaaatcctg
    17101 ttagacgaat ttaagttggt gataaaccaa aacaagctgg aaaaactgct atccatagtc
    17161 actttatacc aggatttagg aggtggttac cgtgggcatt gatcagcaaa agaccgtccg
    17221 taccttgcgt ttatccctga cgtgtatttt tctttttcta attacctggt actaccaagt
    17281 cccagaaagc gcatggactt tagtgaccat atggtttgtc atgtatgaat attccaccgt
    17341 tggtggcgta ctgacaaaaa gctttttgcg cttcgcaggc acggctttga gtgccattta
    17401 tggaatgata gttgtttatt tttgtgccaa taacccgttg attaacatca tggctttagt
    17461 acctggtttg tttctttatg cctatttttt catgggggga gataaaacgt atattggtac
    17521 cataggggct gtaacgctta ctattgtatt gctgaattat aacgacatag atacggctgt
    17581 attgcgggta ttcaatgtca ttatcggtgt cattggttct atgttcatga tccggttttt
    17641 ttatccccaa tatgcaagag ataaagtttt agagattcag ttgaacttta ttactcaact
    17701 tgcaaatctt ttagaaatct atttgaatcc aacccagtct ttggcagctg tccggacaaa
    17761 gtatttggat tatgaacgca agatgcttga tggttttact ttatacaatc gttatattgg
    17821 cgaagccaaa atagaaacca agaaagctcc gtttttcatc ccccactcca ttgccgccat
    17881 gcaacatttc aggcgtctgt ttcgtttgtt cagtgtattt atttattatt tatctactga
    17941 ggaaattcgc tcggatccat gggtatgtga gcaattaagc aaacttatgc acaatttgca
    18001 atcactgcaa cgaaaattgc tgcaacaaga tgcggggccc atagggggag acgttgaggt
    18061 tgcagagcca gaagaagaaa tcgttataga aacacctaat attgaaaata aaatagcaac
    18121 agaagctatt gtcaataata tgcagaaaga aatcgctttg cttgatactg aaattaagga
    18181 aataatatta atttacgatg tttatgatat caggttgaat gagatgtaaa gactcattcc
    18241 aatatcttgg gaagggttca atagaaatga ggatgaaaaa gacacatgag tgacaaaccc
    18301 aaagctggat ttcctgtaat tattttatta tgcgtgtttc tattcattct gatttatcat
    18361 tactcatatt tatttccttt tacaaacaat gcatttgttg ttgcgaatgt tcgtccagtt
    18421 gctgccaatg taaaaggtta tatcaccaat atttatgtta aaaatgaaca ggaagtaaaa
    18481 aaaggccaac cattatttac cgtattcaaa gccccctatg aattggccta caggaaagct
    18541 gtgagcgatg ttcaagaggc gaaagcccaa ttattgttat tcaataaaca agttgagaaa
    18601 acgaaatatc tgttgcaagc acaaaaagag ctttatgaaa aattccgctt tgattatgag
    18661 cataatcatt ctgccttgcg tgatcacgca gtttctaagc tgaccgtcaa tacgatgtta
    18721 aaagaaaaaa atgcggcact aagcaaatta aaagcattgg aaaaagagtt agaggtcaac
    18781 cagcaacaag tcattgtgca aaaaaagaaa attgactctt taagtgcggt gatgaagaat
    18841 gccaaaatcg atttggatga aaccacagtt tatgccaaac aaaatggggc agttcaggat
    18901 atgtttgttg cattgggaac ccccatcaaa acccgaaaac cagtctttgc catagcggac
    18961 accgatacct tgtttatcca ggcaaatttc aacgaaacgg atttaagaag agtacagcca
    19021 ggggataaag tctctatttt cccgagaatg tattttgggt ctaaaattta tcacggagtg
    19081 attctttcca gaaactgggc tgccagccgc ctggaaaccc atcgggcaac gcagatacaa
    19141 attgttcgca atagcgaaag caactggttt ttattgcctc agcgcttgcc ggtacaaatt
    19201 caaataacag attatgaccc ggtgcattac ccattaagta taggcgctag cgcctatgtg
    19261 tatatccata ctcattaaaa ttatgggagg cgttgaaatt aagagtgcaa tatattctgt
    19321 ttttttctat ttccagtatt aatttttagc tgataatttg actgtgacca ttttttagac
    19381 taaccttact ttaataaatt gaacaaaaat tgatcaaaat gtgaatttca tagtttaatg
    19441 acaaggaggt catatgaaaa ttccatttga agggaaatgg cgtgcgacca ttaccaaacg
    19501 tgaggctggt tttagccaac gtgtcgtggt tggaggcgca accagcggga atggcagtta
    19561 caatggtgtt gttggcaaca gctttctttt cgaagacggt tgggttgaac tccagtggaa
    19621 taacaacgca ggttcaggct ggcaggaaag cgctgtaatt agttctgtgg gaatgacgag
    19681 tccgctggtt gttgtaaaat ttgtttcagc cgatgacaat tttccaaatc aacgagacgg
    19741 cgattatgat gatttgcaag tccgttttga acatcttgat gctccttttg aagtaacaca
    19801 aagaccttat gctcttgatc gaggctcact tgccatgttt cctgacggca tttttgatgc
    19861 gagccaaggg attcaataca tgggagtacg aattaaaaac acctggtttt ttgattggca
    19921 aagtaatttt ccgtcaacag gaatgaaaat tggcattgcg ccagtgagtc gttccgcctt
    19981 gtctgctgct ggtattgtgg tgattgatag ttggacgcca caggaacaag aaggttttgg
    20041 gcaaatcatg gatggtggct ttgtgagagt tcctgatcta aaaattaatg aggaaacaac
    20101 aatttacttc aagctggatg tcagtaatgc caatccctcc aaacctgaaa tcggttttgt
    20161 ggcacagcgc gatgcttatg atccacgcta tgatgaacca acacgaatag ttgataaaaa
    20221 aattttcatc agtcgctcca gttacaatcc tgcaaccagg gaattggtga ctgcgatccc
    20281 tgaaggtact ttgtatctga aacttaataa aattgccatt gatcgcaaag gcgctacaaa
    20341 agcagctcag gaattgaaga aatgccttaa acagcatgga ggtggcataa aagggatgtc
    20401 aaaatggctg cataagctaa gccgtggttt ttggcaaaat agtaaagaca aggaacgttg
    20461 gtgcaaggag caaggcaatg atgcattacg taagctttta aaacagcttt tagatggaaa
    20521 ggacattgat atttgtcatc ttaaggctat tttggatcaa tgttgtgaac gcccaggcag
    20581 agattgcgag tgttgtcctt gtggtgatgg agatggcagg ccgggtggtt gggatgatgg
    20641 taccggggtt gatggctggt gccgagtgaa acctgtattc tggttacctc tggattttga
    20701 atataaggta gtccctaacc cggcttacat tggccagttt ggtcctcttg ctttcgagga
    20761 tccctggtgg aaagtagttt taatcatctt ggcgattata ctggctatcg cttcagtcat
    20821 ttatgattat gtttatgcat cagaagatga ccaatacatt attgggcagg ttgtccgtaa
    20881 tggggatgct ccaatcaatc tggttgatgc agcagtgagt aatcttaatg gctctcgtgg
    20941 agtggatctg ggacaactgg atgcccaggg taatgatatt aacaacggca gccccattac
    21001 tgcattagac agtattattc agctcgatcg tacagcggcc ggaagttatt ttggtacgct
    21061 tgacgctgtt ttgggtaatg ttgtctggaa gtcaggggct accagtgcaa caacgagagg
    21121 atttatcagt actctgggaa cttccgtcag cataccctat gacgagcatg atacgctttc
    21181 aggtaccgtt acttacacta acgaagtggt tatcagcgaa cttgctgcga ttccgcaaac
    21241 actctcagat cctggtgact cgggatccgt atgggttgat ctggcaacag cgcgtccagt
    21301 tgctttaaat tacggcagcc caacagactc cagtgataat cgggcttttg ctaatccgat
    21361 acgctctgtt attgacgtgc ttaacttacg gttttaatga aaaggaagta agaaaatgaa
    21421 aagggaattt tttcaaaaac tcgacaagat tcttgatatt gcttccgaac ataagcaaat
    21481 gttgttgcaa tatcctgatg tggtcatggt aggtgttgga cctgagcgtc gcaagggaaa
    21541 aattacaggg gagcctgcga tcattgttac ggtacgtaaa aagcgctcct ccaatgaatt
    21601 aaagaaacgg ggggagaagc ctttgcctca gacaattaag gggattcctg ttgatgtgat
    21661 tgagcttggc aaagccgtgg aagctcccga aattatcgct gttcaggaaa aagcaaaaaa
    21721 aacaattagt aaaattcggg caaaatggtt acaaacacca aatattacag caatagggta
    21781 tggctacaaa gagaaaggag gggaaatcga ttttaacacc attgccatcc agttttttgt
    21841 caaaaagaaa tttcctatgg ctgagctgca aaaccagcaa ataaccccca ttcctgaaaa
    21901 aattaatggg gtgagtactg acgttattca gttatctccc attcgcccgg ctgcggcttc
    21961 cggttccaga ggagatcgcc atgatccgct caaaggaggt gttgcgattg gagtaggaag
    22021 caagccattt cattatggta cttatggtgc cacagtattt gatagaacca ctggcgaacg
    22081 actctttctt agcaatcagc atgttcttga tgcctctcct ggaacagaca ccatacagcc
    22141 ttcgccagtc ttattggatg attcagtgga agtagggttt caattggatg tctgcttacc
    22201 tatccatttt atcaggctgg atactcctaa tactactgtt ggcactgtgt tggctggggc
    22261 tgccgtagct gcagctgttg ccgctgcttt aagtgatgaa attgatccga caaggcgagg
    22321 tcaggaagca acaatgccta cgacaggatc taaaacatta tttgaaaccc aaaaagttaa
    22381 aatggattat cctgaattgc ccattccagg gacacctttt aaagtaaaga cagcttggga
    22441 ttatgaacgc cacaccgatg cgggtattaa gacgttcagt gtcagtgaag aaaaaaagaa
    22501 tccccatgta ttacgcgata aattattaat tacagacaaa aagctttatc atcctgggga
    22561 tactattcgt atttttggtt taatcttgcc agaggaatgt gctcctgcat cacagtccag
    22621 ggacgatcgt caacccatta atccccggga attggaagat ataacccatt atcctaatgt
    22681 ggcttcaagt atcaatcttg acctggtagc ctcaatggat tctgacattg cctcttcgcc
    22741 agcgcgtcgc acgaatacaa gggctgctgt cattaacccc aaacggtgcc gttgcgatcg
    22801 ttatcaccct gtcgccattc ttacaccaat gacggaggat cgtgcttttc cagttgtttt
    22861 gcgtgagcca gtctttgcgc aaaaaaacca gatttttcaa gatttgctaa ccgtagtgaa
    22921 gcaacgagat gatgaaaagt tgctggagcg ggtgtttgtt ctggttcgtt atggttgtat
    22981 atataccggc acattacgtg taaatagcat tccagctggt ccatggaaac attttttcta
    23041 tgttcaaaca gttaacatcg caacaagtga tatgaagccg ctgctggcag cgcaaattat
    23101 tggcggttta cctgtttccc aaaatgctaa agcggacctg gatatagcct gtggcccact
    23161 cacttgggaa gatggtgagt ttgatattga gttgatatag agtgtacagc agggcttatt
    23221 catattggcc aatagccgtg gaataatgtg agttgtattg ataagtaata ccttaaaatc
    23281 aaattatttt catcagctat aaaatagtgc tatgcttttg cggccaaaat ggtacgaaga
    23341 ctattaagtc acattagata cgttttattt tgaaaggaat actctatgtt tggttttttt
    23401 agtgattaca aacaatacat cactctgaga aattttgctg tcgtttataa cgggcttaca
    23461 ggcttggcag tattgtatag cttgtggagt aatcctgagg ctgatccctc tgaatacgtt
    23521 attgatatct ctattcacgc gctgactgct attactttaa tgtgtaaaca agcccccgaa
    23581 tcggttaaag cagttgctat ggcgttaaat acgtataggg gatttgatgc tttgtttaag
    23641 gctatcacct cgcttccttc aacaattccg ggtgttgcaa atgctattga tgtctttaat
    23701 caccggttta attttaagga acttgaaaaa ctcggcaatg aagaaactgc agaaaccaga
    23761 tcagcagttc agcatacaat gtaaagtctg gatcgtcttt tccggtagaa gatggattta
    23821 tcagccattt tctatcgggt tctcttatgt caattgccaa cacgccctag tttattggcc
    23881 gtaatccacc agaaaatttt tcacagctaa ccaatcaata taagctttct ttttatccga
    23941 ggggttgcgc aaaagatagg caggatgata gcttacgata aaaggggtat tgtcatagta
    24001 gtgaatatga ttacgcagct gcttcagagg aagtggttta tcaagcaaga attgtccggc
    24061 gaaacgtcct aaagcaagga ttaagtgcgg tttaatgagt ttaatttggc gggtcaggaa
    24121 tgaactgcat tgagaaattt cctcaagcgc tggatcacga ttattagggg gccggcattt
    24181 caagacattg gcaatgtaga cgtcattctc tgtcatttca atactacgta tcatcttgtt
    24241 taataaaaag ccagccttcc ccacaaaagg ttgccctttc tggtcctcat aaaaaccagg
    24301 cgcttcaccg ataatcatca gttttgcttt tgggtttcca cgagagaaaa cagtttgggt
    24361 acgtgtttta tgcagggaac aacgtacaca ggatgccacc tcatccgcta acgctgctaa
    24421 atgtttttca caagaaggag ttgattcacg catcacccag acttcaattc ccatagtctg
    24481 tagatagtaa ttgttcaata catctgacat aaaaaactca tgatattaat aaaatccgga
    24541 ttatagatta gacttaaatt tttaaacgca ggacttctga ataacttcca caacgagaaa
    24601 tcaaacagtg atgtcaaagt tatttaaaag tcgtataact aatctattgt atcagtccta
    24661 ttttaaagaa agaaacttaa aaaaacatca taaactcact acctaataag aaatagagga
    24721 ttggagttat gaagaatatt gaacggagac tgggaagaat atgaacggcc aaaaggattt
    24781 gaaaccaata gatgttaagc aagtgattgc ctgggcattc tacgattttg ccaattcctc
    24841 ctattttgtg gtgatattta catttgtctt tgcaacctat ttcacaagtc acattgcccc
    24901 taatcctatc ctgggaacgg aattgtgggg ttatacgatt tcgctttctg ctttgctcat
    24961 tgccctggtg agtcctgtcg ttggcgcaat agctgatttt agcgggcatc ataaatcctg
    25021 gctattcttt tttacttatt taggggttat ttcaacagct tgtttgtggt ttgcctatcc
    25081 tgacagccat tctattccct tggttttaac ttgtattttg attagcaatt ttgcattgga
    25141 agtaggaact gtattttata attccttttt ggcaaattta gccccggaaa attatctggg
    25201 gcggatttct ggctgggctt ggggttgtgg ttatctggga gggttgctgt gcctgattct
    25261 atccttattt gtttttgtga aaggtgaatt tggtggatta tggtttggga cagagaattt
    25321 tgccaatatt cgtgctgtca ctgtatttgt ggctttatgg attagtttgt tcagtttgcc
    25381 tttattttta atggtgcaac agaaaacagg tgagcatttg acagtgggta ctgcgattag
    25441 caaaggtttg caagaattgt ggttaacaat taaaagcctg cccaaacaac gtgatctgtt
    25501 cttattttta atagccagaa ttttttatat cgatgggttg aactctatat tggcactagg
    25561 cggtatttat gcggctggca actttcactt gagtgttagc gatattatgc tgtttgggat
    25621 cattttaaat attactgcag gtcttggcgc gggattattc gcctggtttg atgattggat
    25681 tggatctaaa aaaactattc taattgcttt agcgtgcctg acattcactt tttgttttct
    25741 gttaaccatc cactcaaatg cgttattttg ggtttttgca ccagtgtttg gtatttttgt
    25801 tggtcctgtt caggctgcca gtcgtacttt cttggctcgt ttggcaaaac ccgaagaaat
    25861 gacgcgaatg tatggttttt actctttttc tggtaaggca accagttttt taggaccttt
    25921 cctggttagt acaaccactg ctttagctga cagtcagcgt ttagggatgg ctgttcttat
    25981 tccttttttt atcatcggag gcggactatt attattggtt catgaaaatg aataaggaaa
    26041 attccggtaa ggctgatttt ccctgttttg aagttcagta tgagtttgaa agacagggga
    26101 aaaattagcc tctttgtttt tacaagttat ttcttatgat aaactttgtt ctaatatgaa
    26161 aattattata tctctattat ttgctttcat tagcgcctct gggcatgcgt tttcttgcta
    26221 tgatccacag gctatcattc aaaaacccat acagtttgat aaaaaacgga ttgccttaac
    26281 ccgggaatat cagcttactc attatggcat tgattccgag tcgatagaaa ttgagcctaa
    26341 aatgattgta ttgcattgga cttgtatacc aagccttgat actactttcc gcatatttga
    26401 tccccccgct ttacccagca gttcaccaag acgaaatgaa ttgcccgggg atttaaatgt
    26461 atcaagccat tttttagtcg atcgggatgg aactatttat caactgatgc cagaaacatg
    26521 gatggcaagg catgtgattg gcttgaatca ttacgcgatt ggtattgaga atattggcgg
    26581 tgttgatagt aaagacgatt tgactgagga gcaagccaag gcaaatgcct ttcttgtgtg
    26641 ctatctcaag gaaaaatacc cgcaaataaa acatgttatc ggtcataatg aatatttgca
    26701 gtataaagga acggctttgt ggttagagaa ggatccaaat tatcaaaccg ataaaacgga
    26761 tccggggcca actttcgtta aaaaagtaaa acaattaatt gttgcgccat aaataagatc
    26821 atagatcttt atgcgagtaa tgtatgtagg agcttatatt cagcctttaa tttttacttt
    26881 tggattgccc tattgttttt aaataaaaat cgggttttgg gtagtgttgg tattcaaacg
    26941 ttgtaaacat tcctgcgttt aaatgatata aattatggca gtggttagcc cagatgccag
    27001 gatttagcgc atcaaactgt attttcactg tcgaatgtgg ttggactaac agggtatcgc
    27061 gcatggctcc atccaatggc tgaccatcaa tttcggttac ttgaaagaca tgaccatgga
    27121 aatgcatggg gtgagacata gaggatttat tttcatagat catttcaaca cgatcgccga
    27181 aatggattac ttttggcgtg acatctggcc agctttgctt gttcatggtc caaatataac
    27241 cattcatctt gccatcaagc acatagtgca aacgagcatt tattttttta ttggttaaag
    27301 cattttttcc ttttagtttt ttttctaact catagtagtt gacacgtccc gttttattcg
    27361 tggtattggg agatagtatc ggggtaggtg tgttatctgt ttttagaatt agaccggttt
    27421 gtttgtttgt tccttctgcc atagctttaa cagggaatgc tccgcctgca gaaggaatct
    27481 caacaagcaa atccatacga ttaccaacgc caataggaaa agaactatcc acaattggct
    27541 caatgttttc accatcaaca gcaatcaaag tggctgacag tttcccggca tcaattttaa
    27601 aattggtgga ggaagacgca ttgatgattc gtaaccttac ttttttagag gcttgaactg
    27661 aaattacatc aggtgctgcc aatgtttttt tgttggcgag aaacgcatca taggtcacgt
    27721 cattcaaatc gttaccagca tccattttct tcatgtccat tttagcggtt cttaactgat
    27781 caaatattaa ctggggatcc ttgtaagtaa aatcctctaa aaacagaatg acttcttgta
    27841 agtttttatg cgggtcatcc ttgtcataaa taatcaaagg ggcagaaagt tgcttttgct
    27901 cttgaaattt ttgatgcgag tgcatccaat aggttccggc ttgtaataat ctgtagttaa
    27961 ataatttatt ttgccctggc tgaatcagga tttgactcac gtcaggcact ccatcctgat
    28021 cgcttgggac aattaatcca tgccaatgaa gagttattgg tgatgcgctt ttattttcaa
    28081 ctaaaacatg aaacatatct cctttattgg caaagtaacc tttgttgtcc tttgtgccgt
    28141 ccgggtgggt aaaggtgagt ttaaactcct ttgttttttt tcctttacca tcgacctgga
    28201 atttttcctc gctgacggtt atgaccgtcg gtttacgttt gttatctgtt gcatgagagg
    28261 gcttaaaagg agttatcgtg atagttgcta tcatgataaa aaaagatatt tgactcaaag
    28321 tccgcactgt aattttattg attagaattt ttttccaatg gacttcggtg aggggagtaa
    28381 gtcgataaaa atctcttgta ttggtcattt tgatggtctt tccatgtaca tgatagttct
    28441 attattttta tcagttaata acgcagtaag caaatcgctg attttagggg ttattaggat
    28501 taattttgag aatcagtcgc gcaattgcaa ataattctcg tttgtattat tgttctcatt
    28561 tactggaatt acaccatgtc ttcaagttgc aatgattcat gaaggatgag tgattcggcg
    28621 ttttttatag ggaaaataat gaaaagaatt tttttaacat cgatgattgg gatagtttta
    28681 aattctgcat acgcttttga gcaaatgggg ggatgcggag ctcatacctg gcatgaagta
    28741 acctcgaaat cgggttcctg gaaaatcact ttaaaaatga tcagtcagaa tggtcaattt
    28801 gttgaaccca cgtgtgcaga aataactgaa acaggattgg ctcagaataa ccctttgagc
    28861 tatggcctta aattcagcaa agacgcagat tttaacctgg catatacttt aacggctgta
    28921 agtgaagaac agggttttca atcaaaagcc tgtgtgtttg ttatcacggc taatgggcct
    28981 gcgcaaccgg atattcaggc gcttagttat catggggctg aatgcacatg gcgggtaatc
    29041 aaaggggttg gggaaaattt tagtgtcggt tagttctatg aagcggccta aaaccacttc
    29101 ttttttctga aaattaaaaa caatagcaga gcacaaagta cgagcaagcc aagtgcccaa
    29161 aagtagccat atttccagcc taattccggc atccaataga aattcatccc ataaattccg
    29221 gtaattaaac ttaaaggcaa gaatatggct gcgaaggcgg ttaatacttt catggtttca
    29281 ttcatgtttt gcatcaactg gttatcgatt tgtcccaata agccatttaa catatcgcga
    29341 agagaatcta cttcattgac aataaggtgg cagtgatttg ataaattata taaggaggtt
    29401 cgacactgct ctgagatgac taaaatattt cggctggtga gacgcattaa tatttctctt
    29461 attgcaatgg tataacgctt aattttcatg acaccatgct ttaattccaa aacttgcgaa
    29521 taggtgtttt tattagtggt acgaacttgc gcgtcgaatt gatcggctaa ttcttcataa
    29581 gctaaatgta tcttggcata atcattaatt actccttcca gaagcaaaaa taacaaaaaa
    29641 caagatgttt tagcataggg caaggatttt gggcagcttt ttaacagatc aaacaaaact
    29701 gaggaaagtg ttttagaagc agtaaagcaa taattgggtg tgagatgaat aatcaaattg
    29761 ccaaaatcag catcataact gtcattcaac ttcattgagc acaagccttg aatttgcaat
    29821 gtgagcgctt catcgatctc tatggttgtg gatctattgt ctttttctcc acacaattga
    29881 atcacttgct cgggtaatcg taatttctct cgcaattttt taaagatatt tttttggttt
    29941 aaattgctat gtatccaata aattttatct ttatcctgat aatcgatatt taaatcctca
    30001 atcgcaattt tttgcatact atgatttttt aaatcaaatt caatagcgat tgattcaaac
    30061 gaatgcattt caatgctcat ccctatacga ttaatttcct catatagttt aagaatagga
    30121 gacttaaatg atattgacaa actattttta aatagcccgg gttttccaat agactgtgag
    30181 tatcttgtgg ctagctggtt ttctgtttgg ctgttgcctt gttttaattc tatctctata
    30241 tataattcag gttttgttct ggacaatgag tataaaatca gaatccatta atataggtta
    30301 tttttgagca tttaatgaat caagatatgg aaaagattga acagatgtac caactgatta
    30361 tggaaatgaa taaattgaag cttgttttcc gtaataccat aaccaatcct caacgaaagg
    30421 aaagcacagc ggagcattcc tggtcagctt ctatgataac ccttattctc atgaatgaat
    30481 taaaaagaga atttatagac gtcgatgaat taaaaactat gaagttggtt cttatccatg
    30541 atgtcgttga aatttatgct ggagatgttt tggcttttga tgtggaagca agaaaggata
    30601 aggaaaaagt agaattggaa gcgcttgaaa aactaagtgc tgtctatcct tcatttggga
    30661 tagaattgga tagtttatgg catgagtttg aggaaagaaa aagtcttgag gcaaaaatag
    30721 ccaaagcagc cgatgcgatt tgtccaattt tccaacgtct ccaggcaaaa caatcttata
    30781 tcccttttaa tatttcaatg gctcaacttg agaaaacaaa atacccttat tttcaattta
    30841 gtaaaacgtt cagtaacttg tttgaaaaat taaaagcgga tttactcaaa gagcaactta
    30901 tttttttata gcgccatttg catttgcttt caacacatcc tgcccgatgt catgttaaag
    30961 ctccttcaag acagattaaa agttggtgaa tcaaaacgaa tcctctatac ttttaaaaac
    31021 tcttattatc aagaagataa gcattatgta tgatgatcta tggaagcctt atgcatctat
    31081 tgaactaccc tctttaaaat tgggcggcat tgtcttaaat ggtcagttac tctctgaaac
    31141 gcctattcac ttagtgttaa aggctttcaa tcgacatggt ttaattgcag gagcaaccgg
    31201 aacaggtaaa acgaaaacca tgcaggtatt agcagagcaa ttatccctaa atggagtgcc
    31261 cagcttagtg atggacatca agggggatat ttctggcctg gctatgccag gtgacgcttc
    31321 tactaaatta ctggagagaa gtcagctgct acatatagat tttactcccc gaggtttccc
    31381 ggtagagtta ttgactctga ctccacaaga ccccggtgtt ccattacgtg cgagaattga
    31441 tgattttggt cccctgcttt tttcgctgat gctggatctg aatgatactc aggcaggagt
    31501 cgtgactgta ttatttgagt atgccaaggc gaatcaactg aatttagtta atttggatga
    31561 ttttaaatct ttgctccaat ttgctcaaac ggatagaggc aaccagctga ttgccgccca
    31621 atatggcgga gtcgcttcca catccattgg gactattgtc cgtaaaatca tcgagctcga
    31681 gtctcaggga gggaaacagt tttttggtga accttccttt gtcattcagg atttgctgag
    31741 aacggatgca aatggttatg gtgtcatttc catactaaga ttattaaaca tgcaggacaa
    31801 acctaaacta ttttccagtt ttatgctgaa gctattgtct gatgtttatc gccaaatgcc
    31861 ggaattaggt gatccggaca aaccacgatt ggttctcttt attgatgaag cgcatttaat
    31921 ttttaattat tccagcaaag cattacttaa tttaatagaa acaatagtta agctaatccg
    31981 atccaaagga atcggtttga tattttgcac tcaaacgcct aacgatatcc ctgaggctgt
    32041 tttaagccaa ttggggttaa aaatccaaca ctcattacgt gcttttactg caaaagatcg
    32101 aaaagcctta aagttggtat cgcagaattt tccagtatcc agttattata atactgagca
    32161 actactcact actttaagta taggggaagc tttagtgagt gccttggatg ccaagggtca
    32221 accgacacct ttagttcatg cgatgattag agcacctgaa tcacgtatgg ggcctttaag
    32281 caatgaggag ttaaaggttt tactggatca atcgctcctt tttggcaaat attctcaaat
    32341 gatagacgcg ccttctgcga aagagaaatt ggctgagcta atctctgagc aacaagttgc
    32401 aaaacaacag accagtcaga aagaaaacaa ttcctcggtg ttgaatagtt taagtaaaaa
    32461 caccttattc cgacaagtgg tacgtcaagt ttttagggaa ctcaccagag cgattttgtc
    32521 tttgttcaag agtaaaagga actaggccag ttgttgccca tattaaattt ttaacttata
    32581 ccgcgcatgg tcaaaataaa ggcaatataa tcgacaagga tatacatgat aaacatcaca
    32641 ggaacctaca ctgatcaata tcaattggct atggctcaag tgtgtttttt aaaagggcag
    32701 catgagcgca tagcgacatt cgattatttt tttcgtgctc ttccttttaa aggaggctat
    32761 gcgatttttg cgggtttgga ggatttactg gatgttctcg aacaacttcg ctttgaacaa
    32821 caagatatag agtttttaca acatcagaat atgcatcctg actttattcg ctatttgcag
    32881 cagtttaagt tcaatggcac ggtatatacg tcgctcgagg gagatgtggt atttcctacg
    32941 cggccagtgg ttattatcga ggctaatttg attgaagcac aaatcataga aacgttatta
    33001 ttaaatattc ttaattttca gacgcttatt gcgacaaaag ccagacgcat caggcaggtt
    33061 gcgggagaac ggattttaat tgattttgga ttaagaagag cgcagggtgc tggagggtat
    33121 tttgcaagcc gtgcagccat agtcggtggt tttgattcga caagtaatgt cagagccggg
    33181 cgtgattatt caattcctgt atcgggtact atggctcatt cctttatcca aagttacgat
    33241 agcgagatac aagctttttc tgattatgct gaagcatggc cagagaattg catcttgtta
    33301 gtagatactt ataatacgtt gcaaagtggt gttcctaatg caatcaaaat agctaaggaa
    33361 atggagaaga gaggacatag actcaaaggt attcgcctgg atagtggcga tttggcatgg
    33421 ttagccaaga gggctcgcca catgttagac gaggccggtt taaactatgt ggctattaca
    33481 gcttcaaatc aactcgatga atatgtcatc aagaatttgt tagagcagga tgcgcctatt
    33541 gatgcttttg gtgtggggac caatctcgcg attggtgccc cggatgcggc tcttgatggg
    33601 gtttataaat tagcgtttat aaatggtcaa cccaggatta aattatcgga tacccctgca
    33661 aagatcacta ttccttatca aaagcaagtc tacagggtgt tcgaaagaga tgggacatat
    33721 cgaggtgcgg atgttgtctg tctcaaaacg gaaaaaaaaa ttgatatgat gcatcatcct
    33781 tttgaaattc taaaatcgct ttcgataaag cactataaaa aagaagcatt actgcataaa
    33841 gtgatggaaa acggcaaacg gttactacct aaaaaatcac ttggagaaat agccaggttt
    33901 agccagcaat gttttgatcg attgcctttg gaatacaagc gttttattaa tcctcatatc
    33961 tataaagtgg gtttaagtac cgagcttaaa atgaagagag atcaactgat agctgaacac
    34021 tcaagataaa tttatgaaga ctttaatcat cctcgatgtt caaaatgatt tcatgccagg
    34081 cggctcactg ggagtaccga atggtgatgc gattgttccc gttattaaca gcattttgca
    34141 ttgttttgat ctgattgttg cttcacagga ttggcatccc tctaatcata tcagtttcgc
    34201 ctccaatcat actggcagga aaccttttga aaaaatcaag ctggggaatt tagaacaaac
    34261 tttatggcca gatcattgtg ttcagggcag tattggtgct caatttcatg cgcaattgaa
    34321 tactaatcct attgaagcca tttttcgaaa aggaactgac cccaatatag acagctacag
    34381 tggtttttat gataatttgc atcagaaaac taccggactg gccggttacc tacgcgaaaa
    34441 aggcgctaga gaactttatt tttgtggttt gtgcgctgat atttgtgttt attttacaat
    34501 taaggatgct ctcagacagg gatttgattg ctatttgatt gaggatgcaa ctcaagcgct
    34561 tgtgaaagat gatttcataa aaataaaaaa agaattgata aaacagggct ctcacacaat
    34621 taacagttca aatttgctta ataatttaca ataagaaggt ctgatttttt aaatttgcag
    34681 ctttgagctc accaattgat gatggaactc gctgtcgaag gaaccccgtc gtatttttaa
    34741 gacccgctac cataaggttt cgtaatgatt tcaagaaggt ggccattggg atcattaaaa
    34801 tataatcctc gacccccatc attataattc atttgaccta atcgggtttg tcccggatca
    34861 gcccaataat ccattttacg tccgcaaatt cgtttaaaaa tatcatcaaa ttcagtttct
    34921 gttactaaaa aggcataatg ttggggggta atttctttat ctgttttaca gaaatcaagg
    34981 ctcacatggt tgctcatttc aacaaccata aatgggccat atggaattgc ttcaggcaac
    35041 cccaaaattt ctgtaaggaa gtgagctgat ttgtcccgat cttttaccca tacgatagta
    35101 tgattcaatt gtatactcat catgattcct tgttcctgtc gatagaagaa attatttcat
    35161 tactttttat gaaagaaaag aatagccaca tcattaaaat gcatagaatg atgtagatgg
    35221 ctggtaataa atattgtgac attggaagta agcttaatcc caaaataacc actgtaacca
    35281 atcccatatt gatgaagttc ataaccgcgg agccatgggc tttatcggag gtatggctca
    35341 tggcaaatgt cgaagcattt gcaaaaataa aagatagtcc caaatagatg aatatcatgg
    35401 ggaaaaatag cttgataagc gctgaatgat gggttaacgc ggccatcaac attgccagtg
    35461 cggcaaaccc tgagattaaa atgcctaatt ggattcccgc gcgttgcgag tatttttttg
    35521 caaattgagc agaccagatt gagccgaggg tcagacccaa ggaaggtaaa atattggcag
    35581 cgccatactc tgttgtactc attcctagta aattgatggc aataaatggt gccgttgctg
    35641 cgaaaatata gacaaagcag gatgcgccac ccattaatgc tccaccagag attaatggct
    35701 tgtttttaaa ttgacttaca tatccctgga gcagatgttt catttgaaac gcttcaagtt
    35761 gaagattttt ttgtgtttcg ggaagttgcc tgaccatggc aagcagaata gcaccataaa
    35821 cagtacctgc ataaaagcaa ctttcccagc cgtaatgaga gtttaaaatg ccgccaagtg
    35881 caacactcaa accgggtgct atcgcaaaag ccagcatcag ataggaggtt ttttgcgcgg
    35941 ctaattgggg ctcatagcat tcattaacca aagtaaaagt catcttcagt cctacaccgg
    36001 atcctaaggc taatagaaat cgtcctaaaa caagcaatgg gtacatggaa atcattccgg
    36061 ccaatacaca tagaagactg ctgataattt gcaagccaat acctatataa agtgtttttt
    36121 tccttccata gcgattcgct gtagggccat aaataagttg ccctatggca taaccaacca
    36181 aaaaactggt gattgtctgc tgtgcggcat gttctgtgat cccgaaaaat gcagtaatgc
    36241 tgggcaatgc cggtgtaaat aggacagcat tcacggaagc aaaagaaatt aacaacaata
    36301 aagtcacgaa agaaagtttg gtttttggat acatgatgag aataatttta acgattaaca
    36361 gttaagaaca gtataattat tgacaaaata attaacaata tcataattaa ttaacattta
    36421 ttaactatat tgcaataata aaatcgaaat catgggtaaa tttgatcaaa tcacttactt
    36481 tattgctgtt gttgaagagc atggttttgc agcagccgca agaaaatttg gcgtatctac
    36541 agccgctgtt agcaggcaga ttgcacgtct tgaagccgag ctaaaagcag agctgttggt
    36601 gagaacaact agaaaattat cattaactga catcggggct cgctattatc agcaatgcaa
    36661 aaagactctt ttggagttaa atgaggcaga aatagcgatt gctagtagtc ggaatgaagc
    36721 ggtgggcata ttaaatgtta ctagcagccg ttattttgct atgagatttc ttctccctta
    36781 tttaccagaa ttcatggagt taaatccaaa attgcagatt aaaattgaat tggcggaaag
    36841 atttcctgat ttagctcagg aaaacgttga tgtacttttt ggtgtttcta tggagggccc
    36901 ccctgaactg gtgcgtaagc gagtttctac aacacggtat gtgctttgcg catcgccgga
    36961 ttatctggca aaaaatggta ttcccaaatc accttccgaa cttgggaagc accgttatat
    37021 cactcacagc atgcgaaatc ctgataattt ggttacattt aaagacaatg agcagattta
    37081 tgttgaacct gtgttatggc tcaatgatag tcgtgctttg tgtgagtgcg ctttacttgg
    37141 gatgggaatt atcaaattac atgaatacat agtggctgaa gcattgcaag atgggcgcct
    37201 cattgaggtt ttgcctgaat tccaagagtc cagtttatcg gtgtaccttt attatcaggc
    37261 aagccgatat ttacagccta agatacgaag atttattgat ttttttacaa aataacaata
    37321 aaagcaagta ttttgtgtca ttctagcagg tctgagttaa aagcaaaaaa gttcgggggc
    37381 acgtgctttt tttagagaga gatgtcaggt tatgcaaggt ctcattatgt cgcaaattag
    37441 gtgtatctgt taataaatgc ttcaggttgc tttgttagaa taacaacagt ca
//
LOCUS       DBNCWK010000017        37387 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.017, whole genome shotgun sequence.
ACCESSION   DBNCWK010000017 DBNCWK010000000
VERSION     DBNCWK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 37387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37387
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.017"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..4201
                     /gene="sdhA"
                     /locus_tag="AC06IO_000747"
     CDS             <1..4201
                     /gene="sdhA"
                     /locus_tag="AC06IO_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdhA"
                     /protein_id="HIO9873960.1"
                     /translation="TLKSGHFNPRLAPYLEKMNKDGVPFTDFDLDPENISQIKKLINA
                     LYHARLAFLDLENIDIRNYKRTLPDLKLLYSNTIHQAYQAGYLITNLDVDLQEMFKEE
                     IDLILPVIGQLQAFAGEHQECTKRLAESWKDFPISYKAGEVTGIALEQMQPRTGDWDY
                     QFLTQFSAVLPGYIEKLTQYIQQYSSQIKEKEPTLNNEKLEELQNASLKLLNDLEHLK
                     GNDFFISLKVLKYIHIIRNIITLATSSVEQMGHFSDSSQDVIRDNLAQLKYVVLPTLF
                     GLVDKIEDNAMLKPGTLSIPLMEKIKPLYQLLIYYASKPVNFKEKGEELLSIEDSRFL
                     ALRLERTYQRIDEANKALFKIQKAQEALDNFYKILDTPPYNKQPIHQLPKEIKNQLIT
                     YYKIISPYMMDVDPDLNILLIDSFQGPESWSSYIKKPWRWLRGTLPADHASFVLAQKE
                     ALQTLISKKKATQQFHIELNRDLIESVHKQTNLVLFPYSEKTNVFLLDETKALNPANG
                     ATDKLKFRPEKGHNILINPEDLTSEQALDLYQWYRNKREKFRVASKAYNEFITLLKKQ
                     TKTIAETEGRILHLNNLDKETKTRCRNLYNIFQPYFIDGIPPELRASAISFDKFLVHS
                     FSNESSTYDAPAVDLFEKLDEHFQVYFTEIDLQWGKKSNQYLKWAQEKFARENNSTEL
                     EHDEKLESRAHHLIKHTNFSKYIHEFRTELNQIISLFNNAMKAQLKQQPNGIPYPELQ
                     DKNKELAQSAQVIAIKRIFNSLYHVEKIVVELEKLDTRSYESIYVYHLIQAYDHINEI
                     KKLALKLAVDPHFKLIASELLEKAQTLIATIQEHSDAYQVSPLEVPYNHDIKYNALWY
                     TLNAFYISPKHIRSLRNTNYLTTEELNDLHLKAKKATVTVENIIKSSNSYFKLFLQTP
                     AMYSLYREMTNKLNEFISTSHDAVMNNLDQFRSKIFTPMLMEADAWEDKLGLVPGTIS
                     GPLKTITDEYYKGLLHPLALHSKAHIRMICDKTPIDQRIKTTHKKLENATQHLDKIEK
                     NYKHIIKLYEIIKTKGGKTTDDELIETYKKALYKLVKLQKRLPENIDPDPEDYELDTL
                     LNSELKEYEPKLTLIKPLIIASYHHYEGLKATYLMKANTAREKLSYLKELRSTQEQED
                     LLYIEEYTTESFNKQLDAFCNRHIGLQYTDKEYRTKLREYLLTFKESIIQRSKSAEDI
                     NLTIKNLLKEKIKNFERDNFEKYYHLDAVRVALAQFKNYFSLSTAAIEQNNSTFESEE
                     TLAKKTELINKLVDISEDGTLTPKERVDQISFHVKNPNFERIIMDYKQESYFSFTYLK
                     QCILSLLEALCLYTPERRKLFNNLESSVKTQPKINELTKRFGLFAGNESAISTESRAI
                     TPVSSVENNGTIELNQLAPSA"
     gene            complement(4241..4840)
                     /locus_tag="AC06IO_000748"
                     /pseudo
     CDS             complement(4241..4840)
                     /locus_tag="AC06IO_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946124.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0375 family Dot/Icm T4SS effector"
     gene            5096..5482
                     /locus_tag="AC06IO_000749"
     CDS             5096..5482
                     /locus_tag="AC06IO_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873961.1"
                     /translation="MKKWTVLAASSLLMVNAYANESFCGYKDFFHLGDKTHPGIYVVS
                     GYNDSDVVLQIVGPRSFVIRDGFDCRAGYAHVTVAYDNANWCVLDINDGPFMNHPVVS
                     ASCNGLRYINTTYDGFGSYSYSINIE"
     gene            complement(5793..6863)
                     /locus_tag="AC06IO_000750"
     CDS             complement(5793..6863)
                     /locus_tag="AC06IO_000750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIO9873962.1"
                     /translation="MHQKKRSSLELEALLSLFEGSLRTIPFNILAVMVLALGFIYTNE
                     IPIKLLIIWFFLILILSVIRWVASRIIISKEFYITKGKQSLIGFLLLNFFMGLAWSLS
                     YFIFSPYLNDTSEFIFVFILGGLSTGAIASLSIYLPAYYAYVLPMNFPIIAYNLYLFQ
                     FDKIIFSVMYLLFLIIVLLTARLNSKLLSKVRALSITDSLTGLFNRRHFDMVFSNELS
                     RAKRNKYPISLVFIDIDNFKYINDTFGHSAGDSFLIHVSNILKQTLRRSSDSMFRIGG
                     DEYAAILINMPPNEVATVCVSMQEQFNKKNQYKNVSLSMGVISIDSMRVVDQQSAITA
                     ADKTLYQAKKAGKNQIKSKSLG"
     gene            7050..8000
                     /locus_tag="AC06IO_000751"
     CDS             7050..8000
                     /locus_tag="AC06IO_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HIO9873963.1"
                     /translation="MPDHSKFPFNGLPKDIMNKEIGQYLNPSNIAHLAQTSKNNQTLF
                     ESQLYNVSILLFHAARRKDNAVQSILKKDINLLLKRGKVTDYSGRTFEDISAFEYMLW
                     ALDKHGWTIMLECIPIDSNFENIFKELLSQYNKVETDGVTYTLNEKKITEHHFDFQNT
                     IIKELQDQVNSINAPNPNWNVIDKQWIEGVGGAQKLFPMHVVHEYCANRPFAPVPDFN
                     IKPNSSMEFYNWITDFLDHWFLSYFKLGSAFAAYKGMDHIATASSRSATGTAALDLTA
                     MTVLYDVRTRDFIALKSTLENPLMASDNQFVDNKLARIIY"
     gene            complement(8297..8440)
                     /locus_tag="AC06IO_000752"
     CDS             complement(8297..8440)
                     /locus_tag="AC06IO_000752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIO9873964.1"
                     /translation="MEFDYIIVSDGSPGWAFASRLSANPANKLLLLEAGDNLFLGMSI
                     RLQ"
     gene            complement(8578..8988)
                     /gene="fur"
                     /locus_tag="AC06IO_000753"
     CDS             complement(8578..8988)
                     /gene="fur"
                     /locus_tag="AC06IO_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIO9873965.1"
                     /translation="MEESQQLKDAGLKITLPRIKVLQILEQSRNHHLSAEAVYKALLE
                     SGEDVGLATVYRVLTQFEAAGLVSRHNFEGGHSVFELSQGEHHDHLVCVKCGRVEEFV
                     DEIIEQRQKAIAERAHFKMTDHALNIYGICPQCQ"
     gene            9186..9530
                     /locus_tag="AC06IO_000754"
     CDS             9186..9530
                     /locus_tag="AC06IO_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIO9873966.1"
                     /translation="MAKFFMVGKKMRIMTFFICFILTLTLTQCASYDFSRRITQQGNL
                     LPQSKIARLKTGMSKNDVAILMGTSLMSPTFNNDRWDYAYTWRRGKGNLEIRNVVLYF
                     SRGSLVKIEHKP"
     gene            complement(9618..9890)
                     /locus_tag="AC06IO_000755"
     CDS             complement(9618..9890)
                     /locus_tag="AC06IO_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIO9873967.1"
                     /translation="MVKVELVYIANSKNCLHYKMDLKQGATVEEALIKSNIYSICPET
                     RELPVGVYGKRVSVDQVLKEGDRIEIYRPLILDPKEKRRKLAQFKK"
     gene            complement(9877..10311)
                     /locus_tag="AC06IO_000756"
     CDS             complement(9877..10311)
                     /locus_tag="AC06IO_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIO9873968.1"
                     /translation="MTIVKRSRTVPYSCEQMYGLVNDVEHYSEFLPYCAESKILHRDN
                     DEVQATLVIAAAGMSKSFTTRNRLQTNKMIEIRLVDGPFSHLEGFWRFDQEENGCRIS
                     FDLEFEFAGKIFSMLLGPIFDQITDKMVDAFCERAEVIYGKG"
     gene            complement(10308..10955)
                     /locus_tag="AC06IO_000757"
     CDS             complement(10308..10955)
                     /locus_tag="AC06IO_000757"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9873969.1"
                     /translation="MKEPLEKAQACVIWMHGLGADASDMMGLADQLAIEDTALRHVFL
                     DAPRRPVTLNGGMVMPAWYDIYGLGFVDEEDKFGIEQSELLIRKVVDAQYNCGFKPHQ
                     IFLAGFSQGGAMALHTALHMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFD
                     PLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ"
     gene            11145..11507
                     /locus_tag="AC06IO_000758"
     CDS             11145..11507
                     /locus_tag="AC06IO_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9873970.1"
                     /translation="MSILFLARKLASDCGFIEQIQQSHNRNQINYRFYSPRTDLHGSN
                     TNRLYGSPGKGVISQNTIFIYWYRDGKNSYFIECSNRLPKKELEVLHQLLMDNQHSLE
                     EILEASNNRFLSFQTRNT"
     gene            11666..11794
                     /gene="lptM"
                     /locus_tag="AC06IO_000759"
                     /gene_synonym="yifL"
     CDS             11666..11794
                     /gene="lptM"
                     /locus_tag="AC06IO_000759"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIO9873971.1"
                     /translation="MRHIYFALLLIVVTLTSLYLSACGQKGPLYLPEQEKKATTNQ"
     gene            11804..12637
                     /gene="dapF"
                     /locus_tag="AC06IO_000760"
     CDS             11804..12637
                     /gene="dapF"
                     /locus_tag="AC06IO_000760"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIO9873972.1"
                     /translation="MGIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGF
                     DQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGARCIALFARYYGLTAKNKLTVTTK
                     TTLMDLIINEDNSVSVNMGVPRLAPGEIPLLADRQSPEYSLKLNNGNTVNLHAISVGN
                     PHAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETI
                     ACGSGAVAAAAIARLFYNLSDKITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLL
                     S"
     gene            12948..15638
                     /locus_tag="AC06IO_000761"
     CDS             12948..15638
                     /locus_tag="AC06IO_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0365 family Dot/Icm T4SS effector"
                     /protein_id="HIO9873973.1"
                     /translation="MVDFDKPVSENDILASLLLIADLSRPSLNQTLSWEQALKSFLSL
                     DDPLIKTEIFIKWLSQLNPDQLQSSPEIVLLLKHIVEQKLLPYTLKKILIQRENFTTS
                     QINWLCNHCLIVAPDQTTLPDIIKLGSWSWLENYMKRNELLNFDLLKIAILNKDYLAS
                     ISNSKESQLDFIYALNANKFNYQQILELIALAQDSQIKSLLITCLLSKKEYLDNLKEK
                     SFLAHLNQSEFHSPPRLNALIHQLNLSCLTVAQIELLQPEACVTLLCSIPHFHQLSEK
                     QVEKILLKYPKPDMIAYWLNHYSSRPNAHFILAHLMNLVDVHVVDELNKMDSTKKETI
                     IMNMVEHLELFDPTHPLLINQNEEKRLITAIQRYLYGQHHDAYVTYIKLLVYKLLNRN
                     YQFSPQAMQLLICLNNLEEFKELNNKIAYLTNYYLRASAQSGDSSLFYNDSRINITSM
                     TQLVQLAPKIPTKSKEKSLLSSLFCGIKINESSVMTPTNDIPEHPLINKLIKSKRTVK
                     SIDYFLMHYKGDKQVLSKIINDYLSYYVQEGCTEIHRRSIYHLIVLMSLSDISASVRE
                     AIFTAFLNYPELHDEQINFSLFVYDAKRTIRHFGAQASEDSYARVIDLCEGALKKLNP
                     NKHKEIISIATQALTEAKIELSFGENTGFIAQLISRLKRCWYYGWTGFFSPNPPTFVA
                     AIDSPIKSSEKKTSPLLQQRKYFTTKLETNLSIILDDIKSGYTQQKLNELIEALSIFS
                     LQSNPKNELKIRQKLHRLFHYLLLESKNDLTLDAWLIKNQQLLIANRFRLLEMQLMKG
                     ARNEVESLLKEFDEDSAHFSPISKEFTHSLPEQNPYKCEPVSSNQTPGATILDSATEL
                     ATSLKSTLGNLGVFFSNNIMSAIYPNNKSTPQSTSTMTSP"
     gene            15812..16117
                     /locus_tag="AC06IO_000762"
     CDS             15812..16117
                     /locus_tag="AC06IO_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0364 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HIO9873974.1"
                     /translation="MDERRKYFRLKNHGEIKASLDNNPIEIVEISSNGAVVVKQKTDI
                     PKEGVLKLQIHNFIMELCYEVIRAEDNNIVLHFTKEDETNKLFLVLKRLRDERKNKT"
     gene            complement(16227..17072)
                     /locus_tag="AC06IO_000763"
     CDS             complement(16227..17072)
                     /locus_tag="AC06IO_000763"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIO9873975.1"
                     /translation="MNNLAQQIEALPITLAGRFIYRFLPYRRRLIFSNISQVYNDQLN
                     ESQKKRLAKAYYSHLAKSLKEALQLRFMSEKKLRAQVEVIGHEKMLAVVAQKKGVLVV
                     TGHFGNWEFAPLGGVLNFKEFKGQFHFIRRTLRFKFIERIMFKNYYQAGLNVIPKKNS
                     LEQVCVALEQNHAVIFVLDQHASLVNRDGIAVEFFGKKAGTYRSLATISRHTGIPVIP
                     AASYRLPNGKHVLEFHDPIPWKDYDTTQESLYRNTLAYNQALEKIILAHPEQWNWMHK
                     RWKLS"
     gene            complement(17065..18342)
                     /locus_tag="AC06IO_000764"
     CDS             complement(17065..18342)
                     /locus_tag="AC06IO_000764"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIO9873976.1"
                     /translation="MSQANRVFITGRSALTASGATADETWNAIISGQCGIDEINFWEL
                     SEWSHRLGGEIKDFQPSKMLPDRKLIKVISRQDIMGINAAVQAVEHSKMIAYRDSLAD
                     ADLFNEQTAIYVGSPGNKYFQQYDFIPLLAKTHGDMRSFANQLFDEVHPMWLLRILPN
                     NVLAYTGITYGFKGPNHNVTNHAVGGTQAILEAYHAIRSGQAERAVVVAYDIGTEPQG
                     LFYYEKLGVVSNRHLKPFDKEHDGTILAEGAAALVLESEASVKSRAATCYGEIVGGLS
                     ASEATGLFSIEADGQHLASLMGQTLSTSGINSEEIGLIVTHGNGNSKSDDSEALAIKS
                     VFKQEEVPATAFKWSMGHTLCASGVLDAVLTTYALETKCVPGIANFQQIAPTCEGLSV
                     SREHREIISNSYAMMINRGFASMNVCLVIKACE"
     gene            complement(18343..19635)
                     /locus_tag="AC06IO_000765"
     CDS             complement(18343..19635)
                     /locus_tag="AC06IO_000765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIO9873977.1"
                     /translation="MKRRVAITGLGVVSPLGNDVSTTWNNLIAGHSGIDSIKQFDASA
                     FPTTIAAEVKNFSPDTSISGKHTRFIMSFTHFALEAARQAFEDASIFPTKQTSDRWGI
                     VTGSGMMTAEFEYLTRFQQTCAAGGEIDWGKLQSNSRDFYNLVDFGKTTSNSGLSLLI
                     QQYGITGYASSVHTACASGGQALGLAMQVIRRGEADFMLAGGFDSMINPLGLSSFCLL
                     GALSTYNETPQTASRPFDATRNGFVLGEGAAFLILEEWSKAKARGAKIYAELAGEGNS
                     LSSYRITDSHPNGDGAIQAIKRALEDAGVQPRDVDYINAHGTSTKMNDLSETNAIKVV
                     FGDRIANLPVSSTKSQTGHLIAAAGALEAVLSVKSIEQAQVPKTANLKTPDPECDLDY
                     VVDGPREKSLGVVLSNSFGFGGSNSCLLFKHPEFEEAK"
     gene            complement(19640..20536)
                     /locus_tag="AC06IO_000766"
     CDS             complement(19640..20536)
                     /locus_tag="AC06IO_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HIO9873978.1"
                     /translation="MRFLFVDRIVQLSPGEWIKGIKHVTRDDAYLTTDDHGRLCFMPS
                     LIGETLGQLAAWNVMQHNHFTARPVAGVVSSARLYRPAYVGETILLESNIDSLDEKAV
                     QYHSIAKVGNDIIFTIDGALGPMLPMTEFISTEVIRQQFAEIYRPGEWSSVLALSSPV
                     LIEENCSDCGMINSSMQFDRILASEPGISLIAEKRITRSASYFPDHFPNRPVLPMTVL
                     LECKLNLAKEFIARAKYDRAYRISELRKIKMNEFVHPGDVVVCQVKVKQQMDDELILS
                     YRCEVDGKRVCVVDIVLIPKGK"
     gene            complement(20536..20949)
                     /locus_tag="AC06IO_000767"
     CDS             complement(20536..20949)
                     /locus_tag="AC06IO_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIO9873979.1"
                     /translation="MNVAEVYPKVREIIADVLVIDEEEVSLNSSLIEDLGAESIDFLD
                     LVFQLEKEFKIKIPRGQLEKNARGDLAEDEFEKGGILTTSGLQALKNYLSEVPADRFK
                     ENMKVNEIPVLFTVETFCKLVISAIAQQQATETVA"
     gene            complement(21020..21778)
                     /gene="fabL"
                     /locus_tag="AC06IO_000768"
     CDS             complement(21020..21778)
                     /gene="fabL"
                     /locus_tag="AC06IO_000768"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HIO9873980.1"
                     /translation="MSSLVFSGKVGLITGGARGIGKATALKLAQSGCDIAIVYYNSSD
                     EAQSLVNEITGMGRKAVALQADVADCQSVKEMFSQFREHFKHLNFLVSNAASGVLKPA
                     LKMSTKHWRWCLETNALALNHLVTEGLALMPKNSRVIALSSLGASRAIPNYAFIGASK
                     AALEALVRSLSLELASYGITVNTVSAGVVDTDALKHFPNRERLLDEYQAHALADRALT
                     PEDVANAIYLLCLPEAAMINAHTLYVDAGYSQVG"
     gene            21940..23307
                     /locus_tag="AC06IO_000769"
     CDS             21940..23307
                     /locus_tag="AC06IO_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9873981.1"
                     /translation="MKYSWNKTVFPIAAIFSFRLLGLFLLIPVFTLYATNLKGATPTL
                     IGLALGSYGLAQGLLQIPFGMLSDKLGRKPMIAIGLLLFAGGSLLGAVTHSIAGMITA
                     RILQGAGAIGSVLIALLADLTLDEQRTKAMAIVGITIGTSFSLAMIISPALTHYYGLS
                     GVFYLTATLAVLGIMILYLIIPNPSKEQFHADSEPSLSLIKPVVTDQHLQRLNFGIFC
                     QHFILTATFFAIPMLLKYQIENGHLHQQWHFYLPLMLFSFILMVPFIIISERKKRLKT
                     VFVISVLLTTFTQGLLVLTSQNWYWFCILMFVYFVSFNVLEATLPSLVSKLASPGSKG
                     TAMGIYSTSQFLGIFFGGSLAGILYQWYDNQAIFLINMILSCLWFVISLSMKSNVYFS
                     TLILPFTANNEESKLVVNQLLKVAGIKEVILSKDENTLYVRVDNEEYSTGSAEKILHQ
                     STIYH"
     gene            23389..23868
                     /gene="ssb"
                     /locus_tag="AC06IO_000770"
     CDS             23389..23868
                     /gene="ssb"
                     /locus_tag="AC06IO_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIO9873982.1"
                     /translation="MARGINKVILVGNVGADPDVRYLPNGNAVTTLSVATSETWKDKT
                     TGEKQDRTEWHRVVCFNRLGEIAGEYIRKGSKLYVEGSLRTRKWQDQQGQDRYTTEIV
                     ASDIQMLDSKGSSATNYDDMPSFQGTSTPQQASTKNQVIPTSTAQDAFDELDDDIPF"
     gene            complement(23935..24318)
                     /gene="rplQ"
                     /locus_tag="AC06IO_000771"
     CDS             complement(23935..24318)
                     /gene="rplQ"
                     /locus_tag="AC06IO_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIO9873983.1"
                     /translation="MRHRNSGRSFSRTSSHRKAMFSNMCCSLIEHELIRTTLPKAKDL
                     RRYIEPLITVSKSDSVASRRRAFDILRSKSAVGKLFTDLGPRFAKRPGGYIRIIKCGY
                     RDGDNAPMAIVELMDRPVISDDTEE"
     gene            complement(24337..25329)
                     /locus_tag="AC06IO_000772"
     CDS             complement(24337..25329)
                     /locus_tag="AC06IO_000772"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIO9873984.1"
                     /translation="MYTEINEMLTPKVLKVQAESPYKARIVLEPLERGFGHTLGNALR
                     RILLSSMPGSAITEASIDGVLHEYSTIEGVQEDVVDLLLNLKSVAIKLTVGNEAQITL
                     NKEGPCQVTAGDIQLTHGQEIINPELVIANLNEKGKLNMTLKVERGIGFHNTDAFVRY
                     HDDEIEKKTVGKLKIDNSFSPVKKVAYFVDSARVENRTDLDKLTIELETNGTIDAEEA
                     IRISASILQRQLHAFVDMKFEESRADNKERNDFDPVLLRSVDDLELTVRSANCLKAEN
                     IHYIGDLVQRTESELLKTPNLGKKSLTEIKDVLASRSLSLGMKLENWPPASLGE"
     gene            complement(25348..25968)
                     /gene="rpsD"
                     /locus_tag="AC06IO_000773"
     CDS             complement(25348..25968)
                     /gene="rpsD"
                     /locus_tag="AC06IO_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIO9873985.1"
                     /translation="MARYLGPKCKLSRREGCDLLLKSGVRDHKSKCKSEKLPGQHGDK
                     KPRLNSYGIQLREKQKIRRLYGILEKQFRNYYKKAARQKGSTGENLMALLERRLDNVV
                     YRMGFASTRAEARQLVAHKAILVNDKVVNVPSFLVNPGDTVSVRQKAKNQGRIQAALA
                     LSEQRAPCDWITVDTGSFKGTFSTAPTLMDLSSDYNVNLVVELYSK"
     gene            complement(25985..26383)
                     /gene="rpsK"
                     /locus_tag="AC06IO_000774"
     CDS             complement(25985..26383)
                     /gene="rpsK"
                     /locus_tag="AC06IO_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIO9873986.1"
                     /translation="MAITKSKQQKTRKKVKRVVSDGIVHIHASFNNTIVTFTDRQGNA
                     LCWATSGGSGFRGSRKSTPYAAQVATERAAAVAKEYGMKSVAVFVHGPGPGRESTIRE
                     LITQDFKIVEITDVTGIPHNGCKPPKKRRV"
     gene            complement(26407..26763)
                     /gene="rpsM"
                     /locus_tag="AC06IO_000775"
     CDS             complement(26407..26763)
                     /gene="rpsM"
                     /locus_tag="AC06IO_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIO9873987.1"
                     /translation="MARIAGVNIPDHKHVVIALTAIYGIGKTTSLKLCKTVDIDPSVK
                     VSQLSDAQLESLRTEIAKITVEGDLRRVVTMNIKRLMDLGCYRGLRHRRGLPLRGQRT
                     KTNARTRKGRRKGTSS"
     gene            complement(26841..26954)
                     /gene="rpmJ"
                     /locus_tag="AC06IO_000776"
     CDS             complement(26841..26954)
                     /gene="rpmJ"
                     /locus_tag="AC06IO_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIO9873988.1"
                     /translation="MKVRASVKRICRNCKIIKRSGTIRVICKDARHKQKQG"
     gene            complement(26971..28299)
                     /gene="secY"
                     /locus_tag="AC06IO_000777"
     CDS             complement(26971..28299)
                     /gene="secY"
                     /locus_tag="AC06IO_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIO9873989.1"
                     /translation="MKNQKHNGSKPRGGLAELKSRLMFVVLGILVYRLGAHIPVPGLD
                     PAKLANFFSEQQNTIFGLFNMFSGGALSRVTVFAIGIMPYISASIIIQLFSVVSPKLE
                     QLKKEGESGRRKINQYTRYLTLLLSIFQSMGMARWLASQQIAMQPDFSFYFTAVVTLV
                     TGTMFLMWLGEQITEKGVGNGISLIIFSGIVSSMPNAIGSVLQQVKEGQMQALTLVII
                     AAVVIVVTGFVVFMERAQRRIRVNYAQRTQGRKVYAAQTSHLPLKINMSGVIPPIFAS
                     SIILLPATLAQFFSQTKGLGWLSDVGMALSPGQPLYLIVYAAAILFFAFFYAALVFNP
                     KDTADNLKKSGAYIPGIRPGEQTTKYIDSVMTRLTLVGAIYLVLVCLLPQILMYTWHV
                     PFYFGGTSLLIIVVVIMDFVAQVQAHLMTQQYDSLLKKANFRGTKLPGLL"
     gene            complement(28302..28736)
                     /gene="rplO"
                     /locus_tag="AC06IO_000778"
     CDS             complement(28302..28736)
                     /gene="rplO"
                     /locus_tag="AC06IO_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIO9873990.1"
                     /translation="MNLNTLSPDPGSRPSRRRVGRGIGSGLGKTCGKGHKGQKSRAGG
                     YHKINFEGGQMPIQRRLPKMGFKSRVGRTIDEVSLGELAKLNDEVIDLVALRKAGLIN
                     NSIKDVKVILSGELTAAIKLKGLRVTKGARSAIESLGGSIEE"
     gene            complement(28736..28921)
                     /gene="rpmD"
                     /locus_tag="AC06IO_000779"
     CDS             complement(28736..28921)
                     /gene="rpmD"
                     /locus_tag="AC06IO_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIO9873991.1"
                     /translation="MGKKIKITLVKSTIGRKPKHVAIAKQLGLGKTNSSVVHSDTPAI
                     RGLVNEINYLLLVEESA"
     gene            complement(28924..29430)
                     /gene="rpsE"
                     /locus_tag="AC06IO_000780"
     CDS             complement(28924..29430)
                     /gene="rpsE"
                     /locus_tag="AC06IO_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIO9873992.1"
                     /translation="MSFDELPKSDGYQEKLVSVTRTAKVVKGGRVFGFAVLVVVGDGK
                     GKVGFGRGKAREVPIAIQKAMDQAKKNMVYIPLSGTTIFHEITWNYGASKVFMKPASE
                     GTGIIAGGAMRAVLEVLGVQNILAKSIGSTNPSNIVRATIGALTHIGTPDYVAAKRGK
                     TVEEVMAG"
     gene            complement(29440..29799)
                     /gene="rplR"
                     /locus_tag="AC06IO_000781"
     CDS             complement(29440..29799)
                     /gene="rplR"
                     /locus_tag="AC06IO_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIO9873993.1"
                     /translation="MNKQNARHRRGLKAKALIRKTGRSRLVVYRSGVHIYSQIVKSDQ
                     LGDKVLVASSTIDKELRSSLTGKSKVEQASLVGQLLGKRAKAAGITQVAFDRAGYKYH
                     GRVKALAEGAREAGLDF"
     gene            complement(29810..30349)
                     /gene="rplF"
                     /locus_tag="AC06IO_000782"
     CDS             complement(29810..30349)
                     /gene="rplF"
                     /locus_tag="AC06IO_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIO9873994.1"
                     /translation="MSRVAKAPVIHSANVEVTFVDGVITVKGPKGILTQKINKLVNIQ
                     HSKESNKLEFSPASNDPMGWAQAGTARALVRNMVQGVTEGYTVTLELVGVGYRAQSKD
                     KSISLSLGYSHSIEYDLPKGVTVETPNNTTILLKGVDKQVLGQIASEIRAFRPPEPYK
                     GKGVKYAGEQIVRKEAKKK"
     gene            complement(30367..30756)
                     /gene="rpsH"
                     /locus_tag="AC06IO_000783"
     CDS             complement(30367..30756)
                     /gene="rpsH"
                     /locus_tag="AC06IO_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIO9873995.1"
                     /translation="MHDPIADMLTRIRNGQQAKHQQVTLVSSKLKEEIARVLKEEGYI
                     QDFFIETLPNGLKSITLKLKYYHGRPVIEFIKRISRPGLRVYKSYKDLHSIPGFGVAI
                     LSTSKGIMTHVSAKVKGVGGEVICEVA"
     gene            complement(30785..31087)
                     /gene="rpsN"
                     /locus_tag="AC06IO_000784"
     CDS             complement(30785..31087)
                     /gene="rpsN"
                     /locus_tag="AC06IO_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIO9873996.1"
                     /translation="MAKKSMLMRESKRAKLVEKYRQRRNELKQLIKSSDDFQVIMESQ
                     AKLAKLPVNSNPVRYVTRCKQCGRPHAVYRKFNLCRICLRQQLMVGNVPGGRKSSW"
     gene            complement(31100..31651)
                     /gene="rplE"
                     /locus_tag="AC06IO_000785"
     CDS             complement(31100..31651)
                     /gene="rplE"
                     /locus_tag="AC06IO_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIO9873997.1"
                     /translation="MARLKEFYKKDVVTMMMKRFNYSSVMEVPRILKITLNMGVGEAV
                     GDKKVMNHAIEDMTLISGQKPVVTKARKSIAGFKIREGWPIGCKVTLRRERMYEFLDR
                     LISITLPRVRDFRGLNPKSFDGTGNYSMGIHEQIVFPEIDYDKTDGIRGLDICITTSA
                     KTNEEAKALLEAFNLPLKDKDRK"
     gene            complement(31667..31996)
                     /gene="rplX"
                     /locus_tag="AC06IO_000786"
     CDS             complement(31667..31996)
                     /gene="rplX"
                     /locus_tag="AC06IO_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIO9873998.1"
                     /translation="MKRIKSGDEVIVIAGKSKGHIGKVLRVIDDAVVVEGGNLIKKHI
                     KPNPQKPENKGGIIAREAPLHVSNVAHYNPVTKKADKVGFKYLESNGVSKKVRYYKSN
                     NEIIDRI"
     gene            complement(32009..32374)
                     /gene="rplN"
                     /locus_tag="AC06IO_000787"
     CDS             complement(32009..32374)
                     /gene="rplN"
                     /locus_tag="AC06IO_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIO9873999.1"
                     /translation="MIQMQTVLEVADNSGARKVMCIKVLGGSHRRYARVGDVIKVSVK
                     DAIPRSKVKKGAVMRAVVVRTAQGVRRDDGSLIRFDDNAAVLLNNQNEPIGTRIFGPV
                     TRELRERFMKIISLAAEVL"
     gene            complement(32463..32717)
                     /gene="rpsQ"
                     /locus_tag="AC06IO_000788"
     CDS             complement(32463..32717)
                     /gene="rpsQ"
                     /locus_tag="AC06IO_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIO9874000.1"
                     /translation="MSTNSESNARTMIGKVVSDKMDKTIVVMIERTVKHPKYGKIMKR
                     RTKLHAHDENQVCRVGNTVKIRESRPLSKTKSWVLVEVIS"
     gene            complement(32719..32913)
                     /gene="rpmC"
                     /locus_tag="AC06IO_000789"
     CDS             complement(32719..32913)
                     /gene="rpmC"
                     /locus_tag="AC06IO_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIO9874001.1"
                     /translation="MKKIDELRNMSVEELQNELLSLRKDQFNLRMKKASGSLDKTHLI
                     TMVRKSVAKVKTILTEKAGK"
     gene            complement(32913..33326)
                     /gene="rplP"
                     /locus_tag="AC06IO_000790"
     CDS             complement(32913..33326)
                     /gene="rplP"
                     /locus_tag="AC06IO_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIO9874002.1"
                     /translation="MLQPKRTKYRKQMKGRNRGLALRGSKISFGEFGLKAVERGRLTA
                     RQIEAARRAMTRHIKRGGKIWIRVFPDKPITQKPLEVRQGKGKGSVEYWVAQIQPGKV
                     LFEMEGVSKELAMEAFDLAKAKLPFKVMFEERTVM"
     gene            complement(33343..33999)
                     /gene="rpsC"
                     /locus_tag="AC06IO_000791"
     CDS             complement(33343..33999)
                     /gene="rpsC"
                     /locus_tag="AC06IO_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIO9874003.1"
                     /translation="MGQKVNPIGIRLGIIKDWNSKWFAGKRYAEFLIQDIKLRNDLKK
                     KLMAAAVSKILIERPANNAVVTILTARPGVIIGKKGGGIETLRKEISDNLGVPVHLNI
                     EEVKKPELDATLVAEGIAQQLEQRVMFRRAMKRAVTSALKAGAKGIKICVSGRLGGAE
                     IARSEWYREGRVPLHTFRADIDYGTAESKTTYGIIGVKVWIFKGEILPQKKRSTESAQ
                     "
     gene            complement(34002..34337)
                     /gene="rplV"
                     /locus_tag="AC06IO_000792"
     CDS             complement(34002..34337)
                     /gene="rplV"
                     /locus_tag="AC06IO_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIO9874004.1"
                     /translation="MEVTAKLKGAPLSAQKGRLVADMIRNMNVSGALDVLKFTPKKGA
                     KLMLKLLESAIANAENNNGADIDDLKVGMVCVDEATTLKRISPRAKGRANRICKRTCH
                     ITIKVSDEE"
     gene            complement(34347..34625)
                     /gene="rpsS"
                     /locus_tag="AC06IO_000793"
     CDS             complement(34347..34625)
                     /gene="rpsS"
                     /locus_tag="AC06IO_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIO9874005.1"
                     /translation="MARSIRKGPFIDHHLISKVEAAIESKSKKPIKTWSRRSTIVPEM
                     IDLTIAVHNGKDHVPVFITDNMVGHKLGEFAMTRTFKGHSGDRKAKGK"
     gene            complement(34644..35471)
                     /gene="rplB"
                     /locus_tag="AC06IO_000794"
     CDS             complement(34644..35471)
                     /gene="rplB"
                     /locus_tag="AC06IO_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIO9874006.1"
                     /translation="MALLKSKPTSPGKRGEIRVVHHDIYKGKPHAALVEKLKKTGGRN
                     NQGRITVRHIGGGQRQKYRIIDFKRNKDGILGRVERLEYDPNRTALIALITYKDGEKR
                     YIIAPSNLEVGATIQSGADSPISVGNCLPLKNIPVGTTIHCVEMKPGKGAQMLRSAGC
                     SGQLVAKEGVYATLRLRSGEMRKIHVLCRAVIGEVSNSEHNLRALGKAGAKRWRGIRP
                     TVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGLPTKGYKTRSNKRTDTFIVRGRKKK"
     gene            complement(35483..35779)
                     /gene="rplW"
                     /locus_tag="AC06IO_000795"
     CDS             complement(35483..35779)
                     /gene="rplW"
                     /locus_tag="AC06IO_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIO9874007.1"
                     /translation="MNAERLMMVLREPHTSEKATVMADKFKQFTFKVLKNATKTEIKL
                     AVEHIFNVKVKSVSVVNVKGKSKRFKQTSGKRSDWKKAFVSLHADQDIDFTATE"
     gene            complement(35776..36381)
                     /gene="rplD"
                     /locus_tag="AC06IO_000796"
     CDS             complement(35776..36381)
                     /gene="rplD"
                     /locus_tag="AC06IO_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIO9874008.1"
                     /translation="MEITTIDTKSKLKLNKEIFAYTYNEGLVHQAVVTFMNNARSGNS
                     AQKTRSEVSGGGKKPWNQKGTGRARAGTIRSPLWRSGGVTFASKKRDYSQKLNKKMYK
                     RALRSIISELCRTGNLVVVSDFQCDNHKTKDFLNKMNQMEISNALIIMSEVGENEYLG
                     SRNLIDYDICDVTTIDPVSLLRFEKVVVTEAAIKKIEEQLQ"
     gene            complement(36381..37031)
                     /gene="rplC"
                     /locus_tag="AC06IO_000797"
     CDS             complement(36381..37031)
                     /gene="rplC"
                     /locus_tag="AC06IO_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIO9874009.1"
                     /translation="MMIGLLGRKIGMTRVFTPEGVSVPVSVVEVQPNRVSQVKTAAND
                     GYSAVQLTGGTKKSSKVSKPVAGHFAKAQIDAGDMQVEFRIDSEDAFTPGQVISVADV
                     FTAGQYVDVSGLTKGKGFAGTVKRHNFRTQDASHGNSRSHRVPGSIGQNQTPGRVFKG
                     KKMAGHMGNARCTIQSLELVKVDSERNLLLIKGAIPGAPGSRVEIKPAVKKQARGE"
     gene            complement(37066..37383)
                     /gene="rpsJ"
                     /locus_tag="AC06IO_000798"
     CDS             complement(37066..37383)
                     /gene="rpsJ"
                     /locus_tag="AC06IO_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIO9874010.1"
                     /translation="MSSNQNIKIRLKSFDHRLIDLSTREIVDTAKRTGAQIRGPIPLP
                     IRKEKFTVLTSPHVNKDARDQYELRTHKRLVVIVHPTEKTVDALMKLDLAAGVDVQIS
                     LDD"
BASE COUNT        11730 a         7931 c         6108 g        11618 t
ORIGIN      
        1 gactttaaaa agtggacatt tcaaccccag actagccccc tacttggaaa aaatgaataa
       61 agacggggtt ccattcaccg attttgatct tgacccggaa aatatttctc aaatcaaaaa
      121 acttattaac gccctttacc atgcccgttt agcttttctg gatttagaga atattgatat
      181 acgcaactac aaacgaacac tccctgacct caaattactt tatagcaata cgattcacca
      241 ggcctatcaa gccggttatc taatcactaa cctcgatgtt gatttgcagg aaatgttcaa
      301 ggaagagatt gatcttattc tccctgtcat tggacaacta caagcctttg ctggtgaaca
      361 ccaggaatgc accaagcgtt tggcagagtc ttggaaggat tttcctataa gctataaagc
      421 aggagaagtt acaggcattg ccttagagca aatgcaaccg agaacaggag attgggatta
      481 ccaattcctc actcaattca gtgccgtatt acctggttat atagaaaaat taactcaata
      541 cattcaacaa tactcttcgc aaatcaaaga aaaagagcct accctaaata atgaaaagct
      601 ggaagagtta caaaacgcat cattaaaatt actaaatgac ctggaacatc tgaaagggaa
      661 tgactttttt atatccctta aagttttaaa atacattcac attatccgta atatcatcac
      721 attggcgaca agctctgttg agcaaatggg acatttcagt gattcatccc aagacgtcat
      781 tcgtgataat ttggctcaat taaaatacgt tgtattacct actttatttg ggttagtgga
      841 taaaattgaa gacaatgcca tgctgaagcc tggtacactg tctattccgt taatggaaaa
      901 aattaaacct ctataccaac ttttaattta ttacgcatcc aaacccgtca atttcaaaga
      961 aaagggagaa gagttactca gtatagagga ctcccgtttt ctcgctttaa gactggaaag
     1021 aacctatcaa cgaattgatg aagctaacaa agcccttttt aaaattcaaa aagcgcaaga
     1081 agctctggat aatttttaca aaatattgga cacccccccc tataataagc aacccataca
     1141 tcaattaccg aaagaaatta aaaatcaact gattacatat tacaaaataa tcagtcctta
     1201 tatgatggat gtggatcctg atctcaatat tttgttgatc gatagttttc aaggcccgga
     1261 atcctggtct tcttatataa agaagccgtg gcgttggtta agaggcactc ttcctgccga
     1321 tcacgccagt tttgttttag cgcaaaagga agcattacaa actttaatta gcaaaaagaa
     1381 agccactcaa caatttcata tcgaattgaa tagagacctt atcgaatctg ttcacaaaca
     1441 aacaaatctg gttttattcc cttatagtga aaaaacgaat gtctttcttc tagatgagac
     1501 aaaagcacta aaccctgcta atggcgctac ggacaaacta aaattcaggc cagagaaagg
     1561 acataatata ctgattaatc ctgaggattt aacatctgaa caggctcttg acctctatca
     1621 atggtataga aacaaacgtg aaaaattcag agttgccagc aaagcatata acgaatttat
     1681 taccttatta aaaaaacaaa ccaagacaat cgctgaaaca gaaggcagga ttctccattt
     1741 aaataatctt gataaagaga ccaaaactcg ctgccgtaat ttatataata tatttcaacc
     1801 ttattttatc gatgggatac cccctgaact cagagcgtcc gcaataagtt ttgacaaatt
     1861 tttggtccat tccttttcta atgagtcatc cacttatgat gctccggcag ttgacctgtt
     1921 tgaaaaactg gatgaacatt ttcaagttta ttttacagaa attgatttac aatggggtaa
     1981 aaagagtaat caatatttaa aatgggccca ggaaaaattt gccagagaaa ataactcaac
     2041 cgaacttgaa catgatgaaa aactggaaag cagagcccat catttaataa aacataccaa
     2101 tttctctaaa tatatacatg agttcagaac ggagcttaat caaatcattt cattatttaa
     2161 taatgcaatg aaagcccaac ttaagcagca acccaacgga attccttacc ctgaattgca
     2221 agataaaaat aaagagcttg cccagagcgc acaggttatc gcaataaaac gcatattcaa
     2281 cagtctttat catgttgaaa aaattgttgt tgaactggaa aagctggata ccagaagcta
     2341 cgaaagcatc tatgtttatc atttaataca agcttatgat cacattaatg aaattaagaa
     2401 gttagcgcta aaacttgctg tggatcctca ttttaagctc atagcaagcg agttactgga
     2461 aaaagcgcaa acgcttattg caacaataca agaacacagt gatgcctatc aggtatcccc
     2521 cttagaagta ccttataacc atgatattaa atacaatgcc ctttggtaca cactcaatgc
     2581 tttttatatt tcaccaaaac acattagaag cttaagaaat accaactatc tcacgacaga
     2641 agaactcaac gacttacact taaaggcaaa gaaagcaact gttactgtag aaaatattat
     2701 taagagttca aactcctatt tcaaattatt cttacaaacg cctgccatgt attctctcta
     2761 tagagaaatg actaataaac tgaatgaatt tattagcaca tcacatgacg cggtaatgaa
     2821 taatctggat caattccgct ccaagatttt cacccccatg ctgatggaag cggacgcatg
     2881 ggaagataaa ttaggattgg taccaggcac tatctcaggg ccattaaaaa caataacgga
     2941 tgaatattat aaaggcctgt tacatccttt agcgctgcat tccaaagccc atattcgaat
     3001 gatatgtgat aaaacaccaa tagatcaaag aataaaaaca acacataaaa aactcgaaaa
     3061 tgcaacacaa catttggata aaatagaaaa aaactataaa catatcatta agttatatga
     3121 aattattaaa acaaaaggcg gcaaaaccac tgatgatgaa ttaatcgaaa cttataaaaa
     3181 agctctgtac aaattggtaa aactacagaa gcgattacca gagaatatag atcctgatcc
     3241 tgaagattat gaattagaca ctcttttaaa ttcagaactg aaagagtatg aacctaaatt
     3301 aacgctcatt aagcctctca ttatcgcaag ctaccatcat tatgaaggct taaaagccac
     3361 ttatcttatg aaagcaaata ctgccagaga aaagttgagc tatcttaaag agttacgatc
     3421 gactcaggaa caagaagatc tcctttatat agaagaatac actacagaat catttaataa
     3481 acaattagat gctttctgca accgtcatat aggtttacaa tacactgaca aggaatatcg
     3541 cacaaaactt agagaatatt tactgacttt caaagaaagt attattcaac gctcaaaatc
     3601 cgcagaagat attaatttaa ctataaaaaa tttgctaaag gaaaaaatta aaaattttga
     3661 aagagataat tttgagaaat attatcatct tgatgcagta agagtcgctt tagcccaatt
     3721 taaaaactac tttagtcttt ctaccgctgc catcgagcaa aataattcga catttgaaag
     3781 cgaagagaca ctggcgaaaa agacagagct cattaataaa ctggttgata tcagtgaaga
     3841 tggaaccctt acgccaaaag agcgtgtaga ccaaatatct ttccatgtta aaaatcctaa
     3901 ttttgaaaga atcatcatgg attacaaaca ggaaagctat tttagcttta cctatttaaa
     3961 acaatgcatc ttatccttat tggaagcgct ttgcctgtac actccggaga gaagaaaact
     4021 atttaataat ttggaaagct ctgtaaaaac ccagcctaaa atcaatgaat tgaccaaacg
     4081 atttggatta tttgctggca atgagtcagc tatctctact gaatccaggg caattacccc
     4141 agtttcttct gttgaaaata atggaacaat tgagttaaac caattagcgc catcagcata
     4201 aaaatatttg atttaatctt ttaatttacc ataaccttgt ctaggtgttg aatgccatag
     4261 ttttttgtaa tgttaccccc ataaattcct ctacttcatc aaaatgctcc aaactctccg
     4321 gatttgccaa caaagtgtta taaccaagtt cccgcactgg gtcgaggtat tcttgaatgt
     4381 catcgaccag taaaatcgat tccttaggta aataactgta tttttcatga tgcacggatt
     4441 gaataacctg agcctttgtc gttatttcct ttcgaacaag tttcccctct tcaactatca
     4501 ttttactgat aaaaaacaga gaatcctttt taaacccgcc aataccagaa tggaagccat
     4561 caacgcttgc aataaaatca gtcattaaat cgactcaaga ctgaagatca ggacgtccgc
     4621 tcaaaaagta caaaggaata tcagtgtgaa tagaaaactg tactaaacgt ctaaattttt
     4681 ctttatttag atgggtagct ccaattgacc catcaggctg aatatcatac tctccatttt
     4741 ccttccttgc aggaagagca acggtgccgt caaaatcaag aaaaataact ttttcaggca
     4801 aatgccccaa gtccttcttt agaaataatg atgaatacat ttttcctcct gacacttaat
     4861 gtttctatcc cttcaaaata ttgtggagat tctcaatctc tactggtcag cgtatccgat
     4921 tagatcaatc gaaacaaggt aactgatcat ctctttaaca ggaaatattt cctaccctat
     4981 ccagaaatca cttgcagagc aggtttttca aaaacaaaag aacaagatga ttgtttaata
     5041 gccatcgatt ccctataatt cgattccctg aataacaata agaaggtatt ttcgtatgaa
     5101 aaaatggaca gtactggcag caagtagcct tttaatggtc aacgcctacg ccaatgaatc
     5161 attctgtggg tacaaggatt tttttcattt aggtgacaaa actcaccctg gaatttatgt
     5221 ggtcagtggt tataatgact cagatgtcgt attacaaatc gttggaccac gtagctttgt
     5281 aatcagagat ggctttgatt gtcgagcagg ctatgctcat gtcactgtag cttatgacaa
     5341 tgccaattgg tgcgtacttg atattaatga cggtccattt atgaatcatc ctgttgtcag
     5401 tgcttcctgc aatggtcttc gttacatcaa taccacctat gatggctttg gaagttactc
     5461 ctattctatc aacattgaat aaaagtaacg tgagttatgg atagcgatta tataagaagc
     5521 aatcgatatc cagggaccgt ttgggctgag aagatacatg agcatcttct cgaagccccg
     5581 gcaaaaatca ttaaatttca tacaaggctt cgagacagca taaatgcccc cccggcccaa
     5641 acaatccaag atgacgagca tcttatacat aactcacgtt aaaagtaatt aatccagagg
     5701 ccgtctctga cataaaagaa ataaaattgt actctcttat ctcatacttg ctgtaatcaa
     5761 atccaacaat tttaattggc tctcaattct tgctagccta acgatttaga tttaatttga
     5821 tttttccctg ctttttttgc ttgataaaga gttttatccg ctgcagtaat ggcactttgt
     5881 tgatcaacta ccctcataga gtcaatgctt atgaccccca tacttaacga cacattttta
     5941 tattgatttt ttttattaaa ctgctcttgc attgaaacac aaactgttgc cacttcattt
     6001 ggtggcatat taataagaat ggcagcatat tcatctccac caatccgaaa catgctatcg
     6061 cttgagcgcc gtaacgtttg tttcaggata tttgacacat gaattaaaaa actgtcacca
     6121 gcagaatgac caaaagtatc attaatatac ttgaaattat ctatatcaat gaaaaccagg
     6181 ctgataggat atttattgcg tttcgctctg cttagttcat tgctgaacac catatcaaaa
     6241 tgtcggcggt taaataatcc cgtcaatgaa tcggttatag ataatgccct cactttagaa
     6301 agtaattttg agtttaatct tgctgtcaac aatactatga tcaaaaaaag caaatacatg
     6361 acactaaaaa ttattttatc aaactgaaat aaatacaaat tgtaggcaat aattggaaaa
     6421 ttcattggca atacgtaagc ataataggcg ggaagataaa ttgatagaga agcgatagcg
     6481 cctgtcgata atccccccag aatgaataca aaaataaact cactggtatc atttaaataa
     6541 ggggaaaaaa taaagtagct caaactccaa gccaatccca taaaaaaatt cagcagtaaa
     6601 aaaccaatta aagactgctt tccttttgtg atgtaaaact ctttactgat tataattcga
     6661 ctagctaccc aacgaatcac acttaaaatt aaaatgagaa aaaaccagat aataagcagc
     6721 ttgattggaa tctcgttcgt atagataaaa cccaaagcaa gtaccatcac cgccaggata
     6781 ttgaatggga tagttcttaa ggatccctca aataacgaca gtaaggcttc aagttccaat
     6841 gaagaccgct tcttttgatg cataattgtt caaaacccaa gcaccttcat tataatttca
     6901 agtatagact agtttattga gttgctccct tataccgcag taaagtttta atttaaaatc
     6961 cctttaaaag gctacagcct cacgttgata ttcaaagcag attacaatgg tataatgtca
     7021 tccttactta attgcattaa gagttgatta tgccagatca tagtaaattt ccatttaatg
     7081 gattacccaa agacataatg aacaaggaaa ttggtcagta tttaaatcca tctaatattg
     7141 cccatcttgc ccaaacctca aagaataacc aaacactatt tgagtcccag ctttataatg
     7201 tgagcatact cttgttccat gcggcccgtc gcaaggataa tgcggttcaa tcaatcttaa
     7261 aaaaagatat taatttactg ctgaaaagag gtaaagtgac cgattactca ggccgcacct
     7321 ttgaggacat cagtgctttt gaatacatgc tttgggcgtt ggataagcat ggttggacga
     7381 taatgcttga gtgcatccct atagattcga attttgagaa tatatttaaa gaactactga
     7441 gccaatacaa caaggtagaa acagatggag tgacttatac tctaaacgaa aaaaaaatca
     7501 ctgaacacca ttttgatttt caaaatacca ttattaaaga gcttcaagac caggtgaatt
     7561 cgatcaatgc gcctaacccc aattggaatg tcattgataa gcaatggata gaaggagtag
     7621 gcggggcaca gaagcttttc cccatgcacg ttgttcacga atattgcgct aacaggccgt
     7681 ttgctcctgt gccagacttt aatataaaac ccaattcatc catggagttt tataattgga
     7741 taaccgactt cctggatcat tggttcttaa gttattttaa attaggttct gcttttgctg
     7801 catataaagg gatggatcac atcgccaccg catcatcgcg atcagcaaca gggaccgcgg
     7861 ctcttgattt aaccgccatg acagtattat atgatgtaag aacaagagac tttatcgcac
     7921 tcaaatcaac attggaaaat ccgctcatgg cctctgataa tcagtttgta gacaataagc
     7981 tagcgaggat aatttattaa tgcgcgcaac cagaaactgt gtggaggact ctgccacatc
     8041 aactaagcca gctttcgttt ttgtgatctt atccaaaagg gtgcatctag aaaatgaaaa
     8101 cctggctcaa taagtcgtta ctaatattat ggataggcag cgggtattac ttaaccgcct
     8161 tggtaaattc tactgagagt tttattcgtg attttactgc gcaatgtggg gctgtcttta
     8221 ccgcctagca aataatttaa aattgccatt taggatactg aactctaacg tagagttgga
     8281 atatagtgta caatcctcat tgcaagcgga ttgacattcc cagaaataag ttatctcccg
     8341 cttccagcaa caataattta ttggcgggat tggcacttaa cctggaagca aaagcccatc
     8401 ctgggctacc atcactaaca ataatataat caaactccat gtgatgaatc cttaaagaaa
     8461 atagcgtttc accaaaacac tccaattatt gtaatgataa agcactgcaa aagaatatag
     8521 taaatacgct tataatattc cgtgaggtga caagccaatc cagttaaaat atcaaagtca
     8581 ttgacactgc ggacatatac catatatgtt tagcgcatga tctgtcattt taaaatgagc
     8641 gcgttcggca attgcttttt gtctttgttc tattatttca tctacaaatt cctctacccg
     8701 cccacactta acacaaacaa gatggtcatg atgttcgcct tgagacaact caaataccga
     8761 gtgtcctcct tcaaaattat gtctcgatac taatcccgct gcttcaaatt gagtaagaac
     8821 tctatacaca gtagctaaac caacatcctc acccgattcc aataaggctt tatagactgc
     8881 ttctgcactc aaatgatggt ttcttgattg ctccaatatt tgcaatacct tgatacgagg
     8941 taatgtgatt tttaatccag cgtcttttaa ctgttgactc tcttccactt gtgctcctta
     9001 actgtaacga atttcgagct ttgatcatta ttccagcaaa attggattaa taattagcat
     9061 caaggcctaa aataattttc atgctctaaa aaccattagc ggccttaaaa cttagtctgg
     9121 ccgaataatg gcaaatacca aattccacat gatgacaggt gcctttcaat aaatgacatg
     9181 ttattatggc gaaatttttc atggtaggca aaaaaatgcg aataatgact ttttttattt
     9241 gtttcatttt aacattaacc ttaacccaat gtgcatcata tgacttctca agacgcatta
     9301 ctcagcaagg taatttgttg cctcaatcca aaattgcccg gttaaaaaca ggcatgagta
     9361 agaatgacgt tgcaatcttg atgggtacca gcttaatgag tccaacattc aacaatgaca
     9421 ggtgggatta tgcttatacc tggcgacgag ggaaaggtaa cctagaaatt cgtaatgttg
     9481 tgttatattt ttccagaggc tctttagtaa aaatagaaca taagccgtaa tttaatatct
     9541 cccccttgcc taagtgatta gataagggga aaattgtttt taagagttca gtgtcgatta
     9601 aaatgcctaa tttgcctcta ttttttaaac tgtgctaatt tacggcgttt ttccttgggg
     9661 tcgagtatca atggcctata aatctcaatt cgatctcctt ctttcaatac ctgatccaca
     9721 gaaactcgct tcccgtatac tcctacgggt aattctctcg tttcaggaca aattgaataa
     9781 atatttgatt taatgagtgc ctcttcaact gtagctccct gttttaaatc cattttgtaa
     9841 tgcaggcaat ttttagaatt cgctatatag actaattcaa cctttaccat aaatgacctc
     9901 ggctctttcg caaaatgcgt caaccatttt atcggttatc tgatcaaata tgggacctag
     9961 aagcatggaa aatattttcc cggcaaattc aaactccaaa tcaaaagaaa ttctgcaccc
    10021 gttttcttcc tgatcaaaac gccaaaatcc ctctaaatga ctaaaaggac catcaaccag
    10081 acgaatttca atcattttgt tagtttgcaa tcgattacga gtagtgaatg acttactcat
    10141 tccagcagct gctatcacca aagtcgcctg gacttcatca ttgtcacgat gaagtatttt
    10201 gctttccgca caataaggta gaaactcgga ataatgttca acatcattaa ccagtccata
    10261 catttgctca caagaataag gtactgttct tgaccttttt actattgtca ttgtaaaact
    10321 ccttgaacct gcttattaag ccaaaggcta agatctttaa tttcttcaaa acatatggaa
    10381 tgttccatag ggtattgatg aaaagaaact tcattatacc cattagccaa gagccaatcc
    10441 ttactctgta gagtccattt tggcagaact aaaggatcaa attgtcctgc tcccataaag
    10501 atgggagtat tcttatcgag ttgcggctta ttatgcttag ccaagggcaa ataagcggac
    10561 aatgcaataa cgccgcataa tcgctcagtc atatgaagag ccgtgtgcaa tgccatagct
    10621 cccccttgag aaaagccagc taaaaaaatt tgatgaggtt taaagccaca attatattga
    10681 gcgtcaacca ccttgcgtat tagcaattca gattgctcaa tgccaaactt atcttcttca
    10741 tcgacaaaac ctaatccata aatatcatac cacgcaggca taaccattcc tccattcaga
    10801 gtcactggtc ttcgaggagc atcaagaaat acatgcctta aagcggtatc ttctattgct
    10861 agctgatcag ctaaacccat catatcagag gcatcagctc ccaacccatg catccatatg
    10921 acacaagctt gtgctttctc taaaggttcc ttcatataaa cgctcaaaac aaattctcca
    10981 cgcaaaaacg taaattatcg ccaatgcaat agagcagcgc aagtttaaca tttaaaaata
    11041 tattgaacaa tttataagca tattgacgct ggtaattcta ataactattg actttattgt
    11101 ataaatcttt atcattatgt ttattttgta tgttgtgctg ttttatgagt attttgtttc
    11161 tagcaagaaa actggcttct gactgcgggt tcattgagca aatccagcaa agtcataatc
    11221 gtaaccaaat aaattatcgc ttttattccc cccgaactga tctgcatggt tccaatacaa
    11281 acaggttata tggaagtcct ggcaaaggtg tcatttcaca aaatacgatt ttcatatact
    11341 ggtacagaga tggaaaaaac agttatttta ttgaatgttc aaatagacta cctaaaaagg
    11401 aacttgaagt gttgcatcaa ttgctcatgg ataatcaaca ctctttggaa gagatacttg
    11461 aggcatccaa taatagattt ctctcttttc agacacgcaa tacctaagca ctacttcact
    11521 agagaaagac acaaaatacg gaataagcta tctgcctgtc ttactcaagc tcaaaatatt
    11581 cccatataaa ataacaagaa gaatgaatca ttgagtttca aagcaaatct catataatga
    11641 aaatttaact ctataataat acctcatgag acacatttat ttcgcattat tactcatcgt
    11701 tgtcaccctg acttctttgt atttgtcagc ttgtgggcaa aaaggacctt tgtatttgcc
    11761 tgaacaagaa aagaaagcaa caaccaatca atagagacat cctatgggaa taaaatttac
    11821 caaaatgcat ggtttaggca atgacttcat tgtattggat ggagtcaacc aatctattca
    11881 actcactgtt gagcaaatac aaaaactagc caatcgccat accggtatag ggtttgacca
    11941 atgtttgcta atagaatcca gtcaaaccga aggaattgat tttaattatc gaatcttcaa
    12001 cgccgatggg caagaagtag gtcaatgtgg taatggggcg cgatgcatag ctctttttgc
    12061 cagatattat ggtttaacag caaaaaataa attgacagta acaactaaaa ctacgctcat
    12121 ggatttaatc atcaatgagg ataacagcgt gagtgtcaat atgggcgttc ccagactagc
    12181 cccaggtgaa attcctttac ttgctgatcg ccaatcccca gaatattcac ttaaattaaa
    12241 taacggcaat acagtgaatc ttcatgcgat aagtgtaggc aatcctcatg ctgtattgtt
    12301 ggttgaaaat atagatacag cccctgtaaa cagtttggga caacagatta gtttccaccc
    12361 gcaattccca gagcaagtca acgttggatt tatgcaaatt gttaatcatg aaaaaatcaa
    12421 cttacgtgtc tacgaaaggg gttgtggtga aacgattgct tgtggtagtg gtgcggttgc
    12481 tgcggcagcg atagcacgtt tattttacaa cctgtcagac aaaatcaccg ttcatttgcc
    12541 aggaggagat ttgtgtatcc aatggccttg tcccactgct cccattatat taacaggacc
    12601 tgctgctttt gtgtatgagg gcacattact ttcataacaa aattcacaaa attaaaacaa
    12661 gtattgaatt tttttttcaa acagttattc tgaattaatc cacaaatata aaacaggact
    12721 taggaaaaac gctaagatca aggcaaaaaa tatttttaat gagggagttt agataaacta
    12781 aatgaccaat taaaaactgg tttttacgaa aagattcggg ggttgcttaa gtcctgtaaa
    12841 gattccatcg ggagaatttg tgaatattat attccgtggc ttaacatgaa cgaaatgggt
    12901 attaaagttg atagagtagt tttgacattt cggtcaagga ggatcaagtg gttgattttg
    12961 ataaacctgt ctcagaaaat gatattcttg ccagcttatt acttatcgca gatttaagta
    13021 gaccatctct aaatcaaact ctttcctggg agcaagctct aaaatcattt ttatcgcttg
    13081 atgatccact gattaaaacg gaaatattta taaaatggct cagtcaatta aatcctgatc
    13141 aacttcaatc gtccccagag atagtcctcc ttttgaaaca cattgtcgag caaaagctgc
    13201 tcccctacac cctcaaaaaa atattgattc agcgtgaaaa cttcaccaca tcacaaatca
    13261 attggctttg caatcattgt cttatagttg caccagatca aacgacttta cctgatatta
    13321 ttaaactcgg ctcatggagc tggctggaga attatatgaa aagaaatgaa ctattaaatt
    13381 ttgatttgtt aaaaatagcc atcttaaata aggattattt agccagcatc agcaatagta
    13441 aagaaagtca gctcgatttt atctatgcgt taaacgctaa taaatttaac taccaacaaa
    13501 tacttgaatt aattgcttta gcgcaggata gccagattaa atcattatta attacctgtc
    13561 tattaagcaa aaaggagtac ctggacaatt taaaagaaaa atcatttcta gcccatttaa
    13621 atcaaagtga atttcactca ccacctcgac ttaatgcact aattcaccaa ttaaacctga
    13681 gctgtttgac tgtggcacaa atagaacttc tacaacctga agcatgtgtt actttactct
    13741 gttcgatccc tcattttcat caattgtcag aaaaacaggt tgaaaaaata ctacttaaat
    13801 atccaaagcc tgatatgatt gcctattggc taaatcatta ctcatccagg ccaaatgcgc
    13861 attttatttt agcccatttg atgaacctgg tagatgttca tgttgttgac gaacttaata
    13921 aaatggattc cacaaaaaag gaaaccatta ttatgaatat ggtagaacat ctcgaattat
    13981 ttgatccgac tcatcctctt ttgatcaacc aaaatgaaga aaaacgatta attaccgcta
    14041 ttcaacgtta tctctatggc caacatcatg acgcatatgt aacttacatc aagttattgg
    14101 tttataaatt attaaaccgt aattaccagt tctcacctca agcgatgcaa ctcctgattt
    14161 gtttaaataa tctggaagag tttaaagagt taaataataa aatcgcctat ctgaccaatt
    14221 actacttgcg agcatcagca caatccggtg attcgagctt attttataat gattcacgca
    14281 taaacatcac gagcatgaca caactggtcc agctagcccc aaaaattcca actaaatcaa
    14341 aagaaaaaag tcttttaagc agcctcttct gcggcatcaa aattaacgaa agctctgtaa
    14401 tgacaccgac caatgatata cctgaacacc ctttaattaa taaattaata aaaagcaaga
    14461 gaacggttaa atcgatagat tattttttaa tgcattataa aggcgacaag caagtgcttt
    14521 ccaaaatcat caatgattat ttaagttact atgttcaaga agggtgcact gaaatacatc
    14581 ggcgttcgat atatcattta attgttctca tgtctttaag cgatatatca gcttctgtta
    14641 gagaagctat attcacagcg tttctaaact atcctgaatt acatgatgag caaattaatt
    14701 tttctttatt tgtctatgat gcgaaaagaa ctatccggca ttttggggca caagctagcg
    14761 aagacagtta tgccagagtt attgatttat gcgagggagc tctaaaaaaa ttaaatccaa
    14821 ataaacacaa ggaaattatc agtatagcga ctcaagcttt aactgaagca aaaatcgagt
    14881 taagttttgg tgaaaatacc ggatttatcg ctcaactgat ttcacgattg aaacgatgtt
    14941 ggtattatgg ctggactggt tttttttccc ccaacccacc tacctttgtt gcagcaattg
    15001 actcgccaat aaagtcaagc gaaaaaaaaa cctcccctct tcttcaacaa agaaaatatt
    15061 tcactaccaa acttgaaaca aatctgtcga ttatcctcga tgacataaaa tcaggttaca
    15121 cccaacaaaa acttaatgaa ttaatcgaag cattgagtat attttcattg caatccaatc
    15181 caaaaaacga gcttaaaatt cgtcaaaaac tccatcgctt atttcactat ctgttactag
    15241 aaagcaaaaa tgacttaaca ttggatgcct ggcttataaa aaatcagcaa cttttaatcg
    15301 cgaatcgttt tcgtttgcta gaaatgcaac taatgaaagg ggctcgcaat gaggttgaat
    15361 ctttactcaa ggaattcgat gaagactctg ctcatttcag ccctatcagc aaagaattta
    15421 ctcattcttt acccgaacaa aatccgtata agtgtgaacc tgtttcttca aatcaaactc
    15481 caggcgcaac tatcctggat tcagcaactg agttagctac tagcctaaaa agcactttag
    15541 gcaacttggg agtatttttt agcaacaaca tcatgtcagc tatttatcca aataataaat
    15601 ccactcccca atcgacatca acaatgactt caccttgatt aatcttccgg ctgcgcatct
    15661 acagccggat tgattataat aacctgaaag aaacagctgt tctaaaagtt ggatctttcc
    15721 tatttaatca gcagatatca cgctcatccg gttgacctat caggaataat agacctaaaa
    15781 ttaaaaagca tacttaattt atggttaaac aatggatgaa aggcgaaaat attttcggct
    15841 aaaaaaccat ggcgaaataa aagccagctt agacaataat ccaattgaga ttgttgaaat
    15901 atcttccaat ggtgctgtag tagttaagca gaaaactgat attcctaaag agggagtgct
    15961 taaactacaa atccataact tcattatgga actttgctat gaagtgattc gagctgaaga
    16021 caacaacatt gtccttcatt tcacaaaaga agatgaaacg aataagttat ttctggtact
    16081 caaacgatta agagacgaac gaaaaaataa aacataaaaa tttgacataa cttgccccca
    16141 taaaaagtca tttgtgatta agtaggagcc tggagaaaac atcctgggac aaagaataaa
    16201 taagaaaacg aagacaatcc attcgtttaa gaaagtttcc agcgtttgtg catccaattc
    16261 cactgctccg gatgtgccag aataatcttc tccaaagcct gattataggc caaggtattt
    16321 cggtataaag attcttgcgt agtatcataa tctttccaag gaatgggatc atgaaactcc
    16381 aaaacatgct ttccgttagg taatctgtag ctggctgcag gtataactgg aattcctgta
    16441 tggcgagata tcgttgccag gcttctgtag gtgcccgctt tttttccaaa aaactctacc
    16501 gctatcccgt ctctgttcac cagggatgca tgctgatcca atacgaagat tacggcatga
    16561 ttttgttcta acgcaacaca aacctgctcc agagaatttt ttttaggaat aacatttagg
    16621 ccagcttgat agtaattttt gaacataatg cgttctatga acttaaatcg caaggtacgc
    16681 ctgatgaaat gaaattggcc tttgaattcc ttgaagttta atacccctcc caagggcgca
    16741 aactcccaat ttccaaagtg accggtaact actaacaccc cttttttctg tgccacaaca
    16801 gccaacattt tttcatggcc gattacttca acctgagctc gtaatttttt ttcactcatg
    16861 aatcttaatt gcagcgcttc ttttaaagat tttgcaagat gtgagtaata cgcttttgcc
    16921 aagcgctttt tttgagattc attcagttga tcattataaa cctggctaat attggaaaaa
    16981 atcaaccggc gcctgtatgg caaaaaccga taaataaaac gtcccgccag tgtaatgggt
    17041 aatgcttcta tttgttgagc taaattattc acaggctttg atcaccaaac atacattcat
    17101 gctggcaaac cctcggttga tcatcatggc ataggaatta gatattatct ctctatgctc
    17161 acgacttact gacaagcctt cacacgtcgg ggcaatttgt tgaaaattag cgatccccgg
    17221 cacgcatttg gtttcaaggg catatgtagt taataccgca tccaatactc cagaggcgca
    17281 taaagtatgt cccatggacc atttaaacgc agtcgcagga acttcttctt gtttgaatac
    17341 tgatttgata gccagggcct cactgtcatc tgatttactg tttccatttc catgggttac
    17401 tattagtcca atttcctcag agtttattcc tgatgtgctt aatgtctgcc ccatcaatga
    17461 agccaaatgt tgcccatctg cttcaataga aaataatcct gttgcttcag aagcggataa
    17521 gccaccaact atctcgccat aacaagtcgc agccctggac ttcacactgg cttcactttc
    17581 caatacaagg gctgcggcac cttcagcaag aatggtgccg tcatgctctt tatcaaaagg
    17641 ttttaaatga cgattactta ctacacccaa tttttcataa taaaataagc cttgaggttc
    17701 tgtgcctata tcataggcaa ccacaaccgc acgttctgcc tgcccactac gtatggcatg
    17761 atacgcttcc aaaatggctt gtgtaccacc taccgcatga tttgttacat tgtgatttgg
    17821 acctttaaag ccataggtaa tacctgtata ggcaagcaca ttattaggta aaatcctcaa
    17881 taaccacata ggatgcactt catcaaacaa ctgatttgca aagctacgca tatctccatg
    17941 ggttttagcc aacaaaggta taaagtcata ttgttgaaaa tatttattcc ctggagaccc
    18001 aacataaata gcggtttgtt cattaaataa atcagcatcc gccaagctat ctctgtaagc
    18061 aatcatttta ctatgctcaa cagcttgcac tgccgcattg attcccatta tatcctgacg
    18121 ggaaataact tttattaatt ttctatcagg aagcatcttg gaaggttgga aatcttttat
    18181 ttctccgccc aaacgatgtg accattcaga taattcccaa aaattaattt catctattcc
    18241 acactggcct gaaatgattg cattccaagt ttcatcagca gttgcaccag aggcagttag
    18301 agcgctgcgg ccagtaataa atactctatt cgcttgactc atttattttg cctcctcgaa
    18361 ttctggatgt ttaaataaca aacagctatt agaaccgcca aaaccaaacg aattagataa
    18421 tactacccct aaagattttt cacgtggacc atcaacaaca taatccaaat cacactccgg
    18481 atcaggtgtt tttaaatttg ctgttttggg gacttgcgcc tgttctatgg atttaactga
    18541 caataccgct tcaagcgctc ccgcagctgc gattaaatgc cccgtttgac ttttggttga
    18601 gctaactggc aaattggcga tcctgtcgcc aaagacaact ttgattgcat tggtttcact
    18661 taaatcattc attttagtgg atgtaccatg cgcattaata taatcgacat cccgtggttg
    18721 aacacccgca tcctccaacg cacgtttaat cgcttgaata gcaccatcac cgttgggatg
    18781 tgaatcagtt attcggtagg agctcaaaga attcccctct ccggccagtt cagcatagat
    18841 ttttgcccca cgggcttttg ctttactcca ttcttcaagg atcaaaaagg cagccccttc
    18901 acccaggaca aaaccattgc gtgtcgcatc aaaggggcga cttgccgttt gcggagtttc
    18961 attataagtt gataaggcac ccaaaagaca aaagctcgac aaacccaaag gattaatcat
    19021 ggaatcaaat ccacctgcca acataaaatc tgcctcgcca cgacgaatca cctgcattgc
    19081 caatcccaag gcttggcctc ctgaagcgca ggccgtatgg acagaagagg cataaccagt
    19141 aatcccatat tgttgtatta ataaagaaag accagagttt gaagtggttt taccaaaatc
    19201 aaccaaatta tagaaatccc tgctattaga ttgcagcttt ccccaatcaa tttcaccgcc
    19261 tgcagcgcaa gtttgttgaa accgcgttaa atactcaaac tcggccgtca tcatcccact
    19321 gcctgttacg attccccacc gatccgatgt ttgcttggta gggaagatag aagcatcctc
    19381 aaaagcctgg cgtgcagctt ccaatgcaaa atgagtaaaa gacatgataa atcgagtatg
    19441 ctttcccgaa atagacgtat caggtgaaaa attctttact tcagcagcaa tagtggtagg
    19501 aaaagcacta gcatcaaact gcttgatact atctatgcca gaatggccag cgatcaaatt
    19561 gttccacgtg gtggatacat catttcctag aggcgaaaca acccctagac ctgtaatagc
    19621 aactcgtcgt ttcataacct catttacctt ttgggataag cactatgtcg acaacacata
    19681 cccgcttgcc atcaacctca cacctataag acagtattaa ttcatcatcc atttgctgtt
    19741 tcactttgac ctggcaaact acgacatctc cgggatgaac aaattcattc attttaattt
    19801 tacgtaattc gcttattcga taagctctat catattttgc tctagctata aactctttcg
    19861 ccaaattcaa tttacattca agaagtaccg tcatcggtag tacaggtctg ttaggaaaat
    19921 gatccgggaa ataagatgct gaacgagtta tccttttttc agcaatcaag cttatacctg
    19981 gttcactggc gaggattctg tcaaattgca tggaagaatt aatcattcca caatcagagc
    20041 agttttcctc tattaaaaca ggcgaactca aagctaatac agaagaccac tcacctggcc
    20101 ggtatatctc tgcaaactgc tgtctaatga cctctgtact aataaattca gtcataggca
    20161 gcatggggcc taatgctcca tctatagtaa aaataatatc attacctact ttggcaatgc
    20221 tatggtattg aactgccttt tcatccagtg aatcaatgtt tgattcaagc aatatcgttt
    20281 caccgacgta agcaggtcta tacaatcggg cacttgatac tacgccagcg actggtcttg
    20341 cagtaaaatg attatgttgc atcacattcc acgctgccaa ttgccccaat gtttcaccaa
    20401 tcaaagaagg cataaagcaa agccttccat gatcatcagt ggttaaataa gcatcatctc
    20461 ttgtgacatg ctttatccct ttaatccatt ctcctgggga taattgtaca atgcgatcaa
    20521 caaataagaa cctcatcagg ctacagtttc cgttgcttgc tgctgggcta tagctgaaat
    20581 aaccagcttg caaaaagttt ctaccgtaaa caatacagga atttcgttaa ctttcatatt
    20641 ctctttaaat cgatcagcag gaacttcgct caaataattc tttaaagcct gtaatcctga
    20701 ggttgttaaa ataccacctt tctcaaactc atcttcagcc agatcaccac gagcattctt
    20761 ttccaactgg cctcttggga ttttaatctt gaactctttt tccaattgaa aaaccaaatc
    20821 aagaaaatcg atggactcag cacccaaatc ctcaattaaa ctactgttta aagaaacctc
    20881 ttcttcatca attactaata catcagcaat aatttcccta accttagggt aaacttcagc
    20941 tacattcata ttttatcctc tgaaccaaca aagctcgcaa agcttgaaat tatatcataa
    21001 aatttacttt tatcatctac tacccaacct gactatagcc tgcgtcaaca tataatgtat
    21061 gggcattaat catggccgct tcaggcaaac aaagcaaata gatagcatta gccacatctt
    21121 caggtgttaa cgctctatct gccaaagcat gagcttgata ttcatccagt aaccgttctc
    21181 tgtttggaaa atgctttaaa gcatccgtat cgacaacacc tgcagaaaca gtattcacag
    21241 taataccata actagcgagc tccaaactta gcgaacgaac caatgcttcc agagctgctt
    21301 tagaggcacc aataaaagca taatttggaa tcgcccttga tgcacctagg ctggatagtg
    21361 caataactcg actgttttta ggcattaggg caagaccttc cgtaaccaag tggttcaaag
    21421 ccaaagcatt ggtttccaaa caccagcgcc aatgtttggt cgacattttc aaagcaggtt
    21481 tcaatactcc actagctgca ttactcacta gaaaatttaa gtgcttaaag tgttctctaa
    21541 attgagagaa catttctttg acagattgac aatctgctac atcggcttgt aaggcaactg
    21601 cttttctacc catgccggtt atttcattga ccagactttg agcctcatca gagctattat
    21661 aatacacgat agcaatatcg cagcctgact gtgctaactt taatgcggtc gctttaccaa
    21721 ttcctcgtgc accacctgtt atcaatccta cctttcctga aaaaactaat gaactcatac
    21781 gataacccta tagtataaaa tcccaccaaa ctaccgctaa tctgctgtgc tgtcaatgga
    21841 aatatcggga ctctatcgtg cggaatttca taatttatct tgtttccaaa gcacttaatt
    21901 tgtaccatct ccacatgata taatttagac atataatgaa tgaagtattc ttggaacaaa
    21961 actgtttttc ctatagcagc aatattctct tttcgtctcc tgggattatt tttacttatc
    22021 cctgtattca ccctttatgc cactaacctt aaaggtgcta ctccaacatt aattggcttg
    22081 gctttgggaa gctatggatt ggcacaagga ttactacaaa tcccttttgg tatgctttca
    22141 gataaactag gcagaaaacc tatgattgcc ataggtctat tgctattcgc tggaggaagt
    22201 ttgttaggag cagtaactca ttctattgct ggaatgataa ctgccagaat actacagggt
    22261 gctggagcta taggtagcgt gttaattgct ttgcttgctg atttaaccct ggatgaacaa
    22321 cgtacaaagg caatggccat agtagggata actatcggaa catcatttag cctcgcaatg
    22381 ataattagcc ctgctcttac ccactactat ggattatcag gagtgttcta tctcactgca
    22441 actttagcgg ttctagggat tatgatactt tatctcatca ttcccaatcc ctcaaaagag
    22501 cagtttcatg cagatagtga acccagcttg tctctcataa aacctgtggt aacagatcaa
    22561 catttacaac gcctcaattt tggaattttt tgtcagcatt ttattcttac agccactttt
    22621 tttgctatac ccatgctgtt gaaatatcaa attgagaatg gtcatttaca tcagcaatgg
    22681 cacttctatt taccactcat gttattctca tttattttaa tggtaccctt tatcattatt
    22741 tcagaaagaa aaaaacgatt aaaaacagta tttgttatat ctgtactgct gactaccttt
    22801 actcaaggcc tgttggtgct cacatcacaa aattggtatt ggttttgtat tttaatgttt
    22861 gtgtatttcg tttccttcaa tgttcttgaa gcaacattac cttcattagt gtcaaagctg
    22921 gcaagtccag ggagcaaagg cacagcaatg ggtatttatt caacgagtca atttttagga
    22981 attttttttg gtgggtcttt agctggtatt ctttatcagt ggtacgataa ccaagctatt
    23041 tttctaatta acatgattct gagctgttta tggttcgtca tttcgctgag catgaaatct
    23101 aatgtttatt tttctactct tattttgcct tttacggcaa ataatgaaga atcaaaattg
    23161 gtagtcaatc agcttttaaa ggtagccggc attaaagaag ttattttgtc caaggatgaa
    23221 aacaccttgt atgttcgagt agataatgag gaatactcca caggaagcgc tgaaaaaatt
    23281 cttcatcaat ctactattta ccattaacac cttcactttg ggctgctacg aaataactgc
    23341 caaattatgt ataataccta actaactttc gcacaggaga tgatttcaat ggcaagagga
    23401 ataaataaag taatacttgt tggtaatgta ggggcagatc ctgatgtaag gtatttgcct
    23461 aacggaaatg ctgttactac actttcagtt gctaccagtg aaacatggaa agacaaaaca
    23521 acaggtgaaa aacaagatag aactgaatgg cacagagtag tctgttttaa tcgtctgggt
    23581 gaaatagctg gagaatacat tcgcaaaggt tccaagctat atgtcgaagg gagcttaaga
    23641 acaagaaaat ggcaagatca acagggccaa gatagatata caactgaaat tgttgcgtct
    23701 gacattcaaa tgctcgatag caaaggaagt agtgctacta attacgacga catgccttca
    23761 tttcagggaa cctctacccc tcaacaagct tcaacaaaaa atcaagtaat accaacatct
    23821 actgcacaag atgcttttga tgaattagat gatgacatac cgttttaaat gattcaaata
    23881 aaagcccgcg ttaatgcggg ctcaaaatat agattatagc tattaatcta aaatttactc
    23941 ttcagtatca tcactgatta caggtctatc catcaattca actatagcca taggtgcatt
    24001 atcaccatct ctatacccgc atttaataat acgtatatac ccacctggtc gttttgcgaa
    24061 acgtgggcct aaatcagtaa ataatttacc tacagctgat ttagatcgca aaatatcaaa
    24121 agctcgtcta cgtgatgcta ctgaatcaga tttactaaca gtaatcaaag gttcgatata
    24181 tcgacgtaaa tcctttgcct ttggcaaagt ggttctaatt aactcatgct caatcaaaga
    24241 acaacacata ttagaaaaca tagccttacg gtggctactg gtacggctaa aactacgtcc
    24301 ggaattacgg tgacgcataa caaagactcc taaatattac tcgccaaggc ttgctggcgg
    24361 ccaattctca agtttcatac ccagcgataa tgatcgagac gctaaaacgt ctttaatttc
    24421 agttagtgat ttcttaccaa gattaggtgt cttcaataat tcactttctg ttctttgaac
    24481 caaatcgcct atataatgaa tgttttcagc ttttaaacaa ttagctgatc gaacagtcaa
    24541 ctccaaatca tctactgaac gtaataatac aggatcaaaa tcattacgct ctttattgtc
    24601 agcacgtgat tcttcaaatt tcatgtcaac aaacgcatga agctgtcttt gtagtatact
    24661 agcagatatt cttattgcct cttcagcatc gatagtaccg ttagtttcta attcaatagt
    24721 aagcttatct aaatctgttc tgttttctac acgagcactg tcgacgaaat aagccacttt
    24781 cttcactgga gaaaaactgt tgtcaatttt taactttcca accgtctttt tctcaatttc
    24841 atcatcatga tagcgaacaa atgcatcagt attatgaaag ccaatacctc tttcaacttt
    24901 aagagtcata tttaatttac ccttttcatt gagattggct atgacaagtt ctggattaat
    24961 aatttcttga ccatgagtca actgaatatc accagctgta acttgacatg gtccttcttt
    25021 atttaaggta atctgagctt cgtttcctac agtcaatttg atagcaactg attttaggtt
    25081 aagcaacaaa tcaaccacat cttcctgaac accttcaata gtactatatt catgcaaaac
    25141 accatcgata gaagcttctg taatggcact acctggcatg gatgacaata agattcttct
    25201 caaagcattg ccgagggtgt gaccaaaacc tcgctccaaa ggttctaaaa ctattctagc
    25261 tttatagggc gactcggcct gtactttgag aaccttaggc gtcaacattt cattgatttc
    25321 agtatacatt cagctatctc tccgagctta cttagagtaa agttctacaa caaggtttac
    25381 gttgtagtct gaagataaat ccattaacgt tggtgctgta gaaaatgtac ctttaaaaga
    25441 accagtatct acagtaatcc aatcacaggg tgctctttgt tcagataaag ctaaagcagc
    25501 ttggatacgt ccttggtttt tagccttttg tcgaactgat acagtatccc caggattaac
    25561 cagaaaagaa ggtacgttaa ctactttatc atttacaagt attgccttat gtgcaactag
    25621 ttgtctagct tcagcacgtg tactggcaaa acccatacgg taaacaacat tatctaaacg
    25681 tctttccaat agagccatta aattttcacc tgtagagccc ttttgtctag ccgctttttt
    25741 gtaataatta cggaattgct tttcaagaat cccataaagc cttctaatct tttgtttctc
    25801 tctaagctga attccataac tatttaaacg aggtttttta tcaccatgct gtccaggtaa
    25861 cttttcagat ttacacttgg atttatgatc acgaacccca cttttaagta ataaatcaca
    25921 accttcacgt cgtgataatt tacatttagg accaagatat ctagccatta attactcctg
    25981 agtcttatac acgacgtttt ttaggtggct tacaaccatt atgtggtatg ccagttacat
    26041 cggtaatttc aacaatttta aaatcctggg ttattaattc acgaatagta gattcacgac
    26101 caggaccagg accatgcaca aaaactgcaa cagatttcat accatattct ttagcaacag
    26161 cagcagctcg ctcagttgca acttgcgcag cataaggagt actctttcta gaacctctga
    26221 agccagatcc accagaagtt gcccagcata atgcattacc ttgtctatct gtaaaggtaa
    26281 ctatagtatt attgaaagat gcatgaatgt gtactatgcc atcagacact acgcgtttta
    26341 ccttttttcg tgttttttgc tgctttgact ttgttattgc catcttttat tttcctacat
    26401 gaaaaatcaa ctactagtgc cttttctacg gccttttctg gtacgagcat tagttttcgt
    26461 acgctgtccg cgcaacggca atcctcttct gtgtcttaat cctctataac aaccaagatc
    26521 cattagtctt tttatgttca ttgttacaac acgtcgaaga tcaccctcta cagtaatttt
    26581 cgcaatttca gttctcaaag attccaactg agcatctgat aattgagaaa ctttaactga
    26641 aggatctata tcaactgttt tacataattt taatgatgtt gttttaccaa taccataaat
    26701 tgctgttaaa gcgattacca catgtttatg atcaggtata ttaactcctg caatacgagc
    26761 cattagtttc tccgaacgtt atttgttctg tcgaaaggga cagaaggata ctattttaat
    26821 aaaataaaat caagcagata ttacccttgc ttttgtttat gacgggcatc cttacaaatt
    26881 actcgtatag tgccacttct ttttattatt ttacaattgc gacatattcg ctttactgat
    26941 gctcttactt tcattcctaa ctccgataaa tcatagcaga ccaggtaact tagtacctct
    27001 aaaattcgcc ttttttaata aagaatcata ttgctgagtc attaaatgag cttgtacctg
    27061 tgctacaaaa tccataataa caacaactat aatcagcaac gaagtaccac caaaataaaa
    27121 tggaacatgc caggtataca ttaaaatttg tggcaacaaa catactaaaa ccaagtaaat
    27181 ggctccaact aaagtcaaac gagtcatcac tgaatcgata tatttagttg tttgctctcc
    27241 tggacgaatt ccaggaatat atgctcctga ttttttcagg ttatctgctg tatccttagg
    27301 gttaaacact aaggcagcat aaaagaaagc aaagaacaaa atagctgctg catacactat
    27361 caaatataag ggttgacctg gtgatagtgc cattcctaca tcagataacc atcccagccc
    27421 tttagtttgt gaaaaaaatt gagctaaagt tgctggtaat aaaataatgc tagatgcaaa
    27481 tattggtgga atcacacctg acatatttat ttttaatggt aaatgactcg tttgggctgc
    27541 atataccttt ctgccttgcg ttctctgtgc ataattaaca cgaattcgcc gttgagcacg
    27601 ctccataaat acaacaaaac cagttactac aattactact gctgcaatta taactagtgt
    27661 aagagcttgc atctgacctt ccttaacttg ctggaggaca gatcctatag catttggcat
    27721 gctagagaca attccagaaa agataattaa ggaaattcca ttaccaacac ctttttctgt
    27781 aatttgctca cccaaccaca ttaaaaacat agtacccgta actaaagtta caacagcagt
    27841 aaaatagaat gagaaatctg gttgcatagc aatttgttga ctagccaacc atctcgccat
    27901 acccatagac tgaaatattg acaagagcaa tgttaaatac ctggtatatt gatttatttt
    27961 tctacgacct gattctccct ctttctttag ttgctccagc ttgggagaaa ctacagaaaa
    28021 cagttgaata ataattgatg cagaaatata aggcataata ccaatcgcaa acactgttac
    28081 acgagacagg gctccgccag aaaacatatt aaacaagcca aatatagtgt tttgctgttc
    28141 actaaaaaaa ttagctaatt ttgcaggatc taatcctgga acaggtatat gagcacctaa
    28201 tcggtaaact aatatgccaa gaacaacaaa cataagtctt gactttagtt cagctaatcc
    28261 acctcgaggc ttactaccgt tatgcttttg gtttttcata gtcactcttc tatactaccg
    28321 cctaaactct caatagcaga acgagctcct ttagtgactc ttaaaccctt aagcttaatt
    28381 gctgcagtca gctcgccaga taaaattact ttaacatctt tgattgagtt attgattaac
    28441 cctgctttac gtaaagcaac taaatcaatt acttcatcat tcaattttgc aagctcaccc
    28501 aaactcactt catcaatggt tcgacctact ctagacttga aacccatctt gggtaatcgt
    28561 ctttgtatag gcatttgacc accttcaaaa ttaattttgt ggtaaccacc tgctcttgat
    28621 ttttgacctt tatgaccttt gccacaagtt ttgcctaatc cagaaccaat acctctcccc
    28681 actcgtcttc ttgagggcct ggaacctgga tctggcgata atgtatttaa attcattatg
    28741 cactctcctc aaccaacaac aggtaattaa tttcatttac aagtccacga attgcaggag
    28801 tatcactatg aaccacactt gaatttgttt tacctaaacc cagctgtttg gctatagcaa
    28861 catgttttgg ttttctacct atcgtgcttt taaccaaggt tattttaatt ttttttccca
    28921 taattagcct gccattactt cttcaactgt ttttccacgt ttggctgcta catagtccgg
    28981 cgtaccaatg tgtgtcaatg caccaatagt ggcacgaaca atgttacttg gattggtaga
    29041 accaatgctt tttgcaagaa tattttgcac acccaacact tccaaaacgg cacgcatagc
    29101 gcctccagca ataattcctg taccttcact tgctggcttc ataaacacct tggaagctcc
    29161 gtagttccat gtgatttcgt ggaatattgt agtacctgaa agaggaatgt aaaccatatt
    29221 tttcttggct tggtccattg ctttttgaat tgctattgga acctctcttg ctttacctcg
    29281 tccaaaacct actttaccct ttccgtcacc tacaactact aaaacagcaa aaccaaaaac
    29341 tcgtccaccc tttacaactt tagccgtacg agttaccgat accaattttt cttggtatcc
    29401 atctgacttg ggtaattcat cgaatgacat aatcgttcct taaaaatcta atccggcttc
    29461 gcgcgcacct tcagcaagtg ctttaactct gccatgatat ttataacctg ctctgtcaaa
    29521 tgcaacctgt gtaatgcctg cagccttagc acgcttaccc aacagttgac caactaaact
    29581 ggcttgttca actttacttt ttccagtcaa actggatctc aattctttat ctatagtaga
    29641 tgaagctacc aacaccttat caccaagctg gtcagacttg acaatttgag aataaatatg
    29701 cacaccactt cgataaacaa ctaatcttga gcggcctgtt ttgcgtatta aggctttggc
    29761 ttttaatcca cgtctatgtc gtgcgttttg cttattcata gttaacacct tatttcttct
    29821 tagcttcttt acgaacaatc tgctctccag catatttcac acctttccct ttataaggct
    29881 ctggtggtcg aaatgctcta atttcagaag ctatttgtcc aagaacttgc ttatcaacgc
    29941 ccttcagcaa aatggttgtg ttattaggag tctctacggt tacacctttt ggtaaatcat
    30001 actctataga atgtgagtat cctaatgaca aactaattga cttgtcttta gattgcgctc
    30061 tatatcctac accaaccaac tctagtgtaa ctgtatatcc ctcagtaact ccttgaacca
    30121 tgtttctaac caaggctcta gctgtaccag cttgtgccca tcccattgga tcattagaag
    30181 caggtgaaaa ttctaatttg tttgattcct tactatgttg aatattaacc aatttattaa
    30241 ttttttgagt taatattcct ttggggcctt ttacagttat tactccatca acaaatgtta
    30301 cttcgacatt tgctgaatga attactgggg cctttgctac tctagacata tcattcctcg
    30361 caagtattaa gccacttcac aaatgacttc gccaccaaca ccttttactt ttgctgatac
    30421 atgggtcatt atacctttag acgtagaaag aatagccaca ccaaatccag ggattgaatg
    30481 caaatcctta tatgatttat atactctcaa acctggacga ctaatgcgct taataaattc
    30541 aattaccggt cttccatgat agtactttag ctttaaagta attgacttca ggccatttgg
    30601 taatgtttca ataaaaaagt cttgtatata tccttcttcc tttaaaactc gagctatttc
    30661 ttcctttaat ttagaagaaa ccaatgttac ttgctgatgt ttagcttgtt gaccatttct
    30721 aattctagtc agcatatcag caattggatc atgcatactc acaattattc tccaataaaa
    30781 tagtttacca gcttgatttt ctaccgccag ggacattgcc caccataagc tgttgtctta
    30841 aacaaattct gcaaagatta aacttgcgat aaactgcgtg tggtcttcca cattgcttgc
    30901 atcgagtaac atagcgaaca ggatttgaat taactggtaa ttttgctagt tttgcctgac
    30961 tttccattat tacctggaaa tcatcagatg atttaatcaa ttgttttaac tcgtttcggc
    31021 gttgtctgta cttttctacc agcttcgcac gctttgactc tcgcatcagc attgattttt
    31081 tagccacaat ttcacccttt tattttctat ctttgtcttt cagtggaaga ttaaatgctt
    31141 ctaacaaagc tttagcttct tcatttgttt tagcacttgt tgtaatacaa atatctaaac
    31201 cgcgtattcc gtctgtttta tcataatcaa tttcaggaaa cacgatttgc tcatgtattc
    31261 ccatgctgta gtttcctgtt ccatcaaaag attttggatt caaaccacga aaatctctta
    31321 cacgaggtaa agtaatagat attaatctat ctagaaactc atacatacgt tcacgtctga
    31381 gcgttacctt acaacctatt ggccatccct ctcttatttt aaatccagct attgattttc
    31441 tagcttttgt aacaactggt ttctgaccag aaatcagcgt catgtcttca atcgcatgat
    31501 tcatgacttt tttgtcacca acagcttctc caactcccat attcagagtt attttgagaa
    31561 tcctgggtac ttccatcaca cttgaatagt tgaaccgttt catcatcata gtgacgacgt
    31621 ctttcttata aaattcttta agtctcgcca tttcaatcac ctaactctat atacggtcaa
    31681 ttatttcatt gttagactta taatatctaa cctttttgct aacaccatta ctctcaagat
    31741 atttaaagcc gaccttatca gccttttttg taacaggatt atagtgagca acatttgaaa
    31801 cgtgtaatgg agcttcacgt gctataatac cacccttatt ttcaggcttt tgtggattag
    31861 gcttgatatg ctttttaatt agatttccgc cttctactac aacggcatca tcaattaccc
    31921 ttaaaacctt acctatatga cctttacttt taccggcaat tacgattact tcatcgcctg
    31981 atttaatacg tttcatatct aatccttctt acaatacctc tgcggcaaga gaaattatct
    32041 tcataaatct ttctcttaac tcgcgtgtaa ccgggccaaa tatacgtgtt cctattggct
    32101 cgttttggtt gtttaaaaga actgctgcat tatcatcaaa tcgaatcaat gaaccatcat
    32161 ctcttcgaac accttgagca gttctaacga ctaccgctct cattacagca ccttttttta
    32221 ccttacttct tggtatagca tcttttacgc ttactttaat tacatcacct acacgggcat
    32281 accgtctgtg tgatccacca agtactttaa tacacattac tttacgtgct ccgctgttat
    32341 cagctacttc aagcactgtc tgcatttgga tcatttttct ctccagatct aatttcgacc
    32401 agaaaaaggc tgctgattat atcaggcctt ttagggtttt aaaagtaaaa caattaagtt
    32461 acttatgaaa ttacttcgac taatacccag cttttcgttt tagagagtgg tcgtgactca
    32521 cggattttta cggtattacc aactcggcaa acttgatttt catcatgtgc gtgtagttta
    32581 gttcttcgct tcatgatttt cccatacttt ggatgtttca cagtacgttc aatcattact
    32641 acaatagttt tatccatttt gtcgctaact acttttccaa tcattgttct ggcatttgat
    32701 tcactattag tagacatttc acttacctgc cttttcagtc aagattgttt taacttttgc
    32761 cactgactta cgcaccatag tgatcagatg tgttttatct agtgagccac ttgctttttt
    32821 cattcgtaaa ttaaattgat ctttacgtaa tgaaagcaat tcattttgca attcctcaac
    32881 tgacatattg cgcaattcat ctattttttt cattacatta ccgtccgctc ttcaaacatc
    32941 actttgaaag gaagttttgc ttttgcaagg tcaaaggctt ccattgcaag ctctttgcta
    33001 acgccttcca tttcaaataa aaccttacct ggttgtattt gggctaccca atactcaaca
    33061 cttcccttac ccttaccttg tcttacttca agtggttttt gtgtgattgg tttatctgga
    33121 aatactctaa tccaaatttt accaccacgc ttaatatgtc gtgtcatagc ccttcgcgct
    33181 gcttcgattt gtctagcagt taaacgtcca cgctctacag cctttagacc aaactcaccg
    33241 aaactaattt tactaccacg aagagccaaa cctctattgc gacccttcat ttgttttcgg
    33301 tacttagtac gctttggttg taacataatt tctaatcctc actcactggg cgctttcagt
    33361 tgatcttttc ttttgcggga gaatttcacc cttaaagatc cagactttta cgccaataat
    33421 tccgtaggta gtcttggatt cagcggtacc ataatcaata tcagctctaa aagtatgtaa
    33481 aggcactcgt ccctctctgt accattcact tcttgcaatt tcagcaccac ccaatcggcc
    33541 acttacacaa attttaatac cctttgctcc agctttcaga gcagaggtaa ctgcacgttt
    33601 catagctcgt ctgaacatga cacgttgctc taactgctga gcaatacctt cagccaccaa
    33661 agtagcatcc aactcaggct ttttcacttc ttcaatattc aaatgaactg gcacgcccag
    33721 gttatcagaa atttctttac gaagtgtttc aattcctccg ccttttttgc cgataataac
    33781 acctggtctt gctgttaaga tagttacaac agcattatta gctggtcgct caataagtat
    33841 cttgctaaca gctgcagcca tcaatttctt tttaagatcg ttacgtagtt ttatatcctg
    33901 aattaaaaat tcagcatatc gctttcctgc aaaccatttt gagttccaat ctttaattat
    33961 tccaaggcgt atacctattg ggtttacttt ttgtcccatt gctattcctc gtcagacact
    34021 ttaatagtta tatggcaggt acgtttgcaa attcgatttg ctctaccctt agctctggga
    34081 ctaatacgtt ttaaagttgt tgcttcatca acgcatacca tgcctacttt cagatcatca
    34141 atatcagctc cattattatt ttcagcattt gctatagctg actctaataa tttaagcatt
    34201 aatttagcac ctttttttgg tgtaaacttg aggacatcaa gcgcaccaga cacattcata
    34261 ttacgtatca tatcggctac taatctaccc ttttgtgcgg ataaaggagc gccttttaat
    34321 ttagctgtta cttccatcat atcctcttac ttacctttag cctttctgtc cccagagtgg
    34381 cccttgaatg tacgagtcat ggcaaactca cctaatttat gaccaaccat attgtctgta
    34441 ataaatacag gaacgtggtc tttgccgtta tgcacagcaa ttgttaaatc aatcatttca
    34501 ggaacgattg tagaacgtct agaccaagtt ttaattggct tttttgattt tgattcgatt
    34561 gcagcttcta ccttgctgat caaatgatgg tcaataaatg gaccttttct aattgaacga
    34621 gccacttgat atcctcatat taattatttc ttcttacgtc ctctgacaat aaatgtgtca
    34681 gtacgcttat tgcttctagt tttataacct tttgttggta atccccatgg agataccgga
    34741 tgacgtccac ctgatgttcg tccttcacca ccaccatgtg gatgatcaac agggttcatg
    34801 gcgactccac gaacagtggg acgtatccct ctccatcgtt tggcaccagc tttacccaat
    34861 gctcttaggt tgtgttcgct attacttact tcaccaatta cagctcggca taaaacatgt
    34921 atcttgcgca tctctcctga acgtaatctt aaagttgcat aaacaccttc ttttgcaaca
    34981 agttggccac tgcaaccagc acttctgagc atttgcgcac ctttaccagg cttcatttca
    35041 acacaatgta ttgttgtacc tacaggtata ttttttagtg gcagacagtt tcctacacta
    35101 attggtgaat cagctccact ttgaatagta gctccaactt ccagatttga tggtgctata
    35161 atatagcgct tttcaccatc tttgtaggtt atcagtgcaa ttagcgctgt tctatttgga
    35221 tcatattcca atctctcaac acgacctaat ataccatcct tattgcgttt aaagtctata
    35281 attcgatatt tttgacgttg tccaccacca atatgtctaa cagttatacg accttggtta
    35341 tttcttccac ctgttttttt tagtttttca actaatgcag catgtggctt tcctttatag
    35401 atatcgtggt gtacaacacg tatttcacct cgttttccgg gtgaagtcgg tttagatttt
    35461 aatagtgcca tctttatctg ccttattcag tagctgtaaa gtcaatatct tggtctgcat
    35521 gaagagagac aaatgccttc ttccagtcac ttcgcttacc actggtttgt ttgaagcgtt
    35581 tagatttacc cttcacattg acaacagata cacttttaac ttttacatta aatatatgtt
    35641 caactgctaa tttaatttca gtcttagtcg catttttcag tactttgaaa gtgaactgtt
    35701 taaacttatc ggccatgaca gttgcctttt cagaagtatg tggttctcta agaaccatca
    35761 tcaatctctc agcgttcatt gtaactgttc ctcaattttt ttaattgcag cttctgtaac
    35821 aacaactttt tcaaatcgta gtaaggaaac aggatctata gttgtaacat cacagatatc
    35881 gtaatcaatc aggtttcttg aaccaaggta ttcattttca ccaacttcac tcattataat
    35941 cagagcatta cttatttcca tctgattcat tttattgagg aaatccttgg tcttgtgatt
    36001 atcacattgg aaatcactaa caaccaccaa gttgccagtg cggcaaagtt cggagattat
    36061 actacgtaat gcacgtttgt acattttttt attaagcttt tgtgaataat ctctcttttt
    36121 agatgcaaat gttactccac cacttctcca taatgggctg cgaatggttc ctgctcgcgc
    36181 acggcctgtt cccttttggt tccagggttt tttaccacca ccactaactt cagaacgagt
    36241 tttctgagcg ctattaccgc ttcgagcatt gttcatgaaa gtaactactg cttgatgaac
    36301 caagccttcg ttgtatgtat aagcaaatat ctctttattt agctttaatt ttgactttgt
    36361 atctattgta gtaatttcca tcactcacct cgtgcttgct ttttgactgc aggcttaatt
    36421 tctactctag aacctggagc accaggaatt gcacctttaa tgagcaataa atttctttca
    36481 gagtcaactt ttaccaattc cagactttga attgtacatc gagcattacc catgtgaccg
    36541 gccatttttt tccctttaaa aacacgacca ggggtttggt tttgtccaat agaaccaggt
    36601 actctatgtg atcttgagtt accatgcgat gcatcttgag ttctaaagtt atgtcgtttt
    36661 acagttcctg cgaaaccttt acctttagtc agacccgata catcaacata ttgtccggca
    36721 gtgaatacat cagcaacaga tatgacttga cccggtgtaa atgcatcctc tgaatctata
    36781 cgaaattcta cttgcatatc acctgcatca atttgtgctt tagcaaaatg cccggccact
    36841 ggcttactca ctttgctaga tttctttgta ccaccagtta attgtacagc tgaataacca
    36901 tcattcgctg ctgtttttac ctgagaaacg cgattcggct gaacctcaac aacagaaaca
    36961 ggaacagaaa ctccttctgg tgtgaagaca cgcgtcatac cgatcttacg gcctaataaa
    37021 ccgatcatca ttgtaacacc tctttaatat cctttgcctt tacatttagt catcaaggct
    37081 tatctgaaca tcaaccccgg cagccaaatc caatttcatt aaagcatcta cagttttttc
    37141 agttggatga acaataacga ccaggcgttt atgagtacgt aattcatatt gatctcttgc
    37201 atctttattt acatgcggtg aggtcaatac agtaaatttt tcttttctga taggcaaagg
    37261 aattggtcca cgtatttgtg cgccagttct tttcgcagta tcaacgattt ctcgtgttga
    37321 tagatcaatc aaccgatgat caaaggattt taatcttatt ttaatatttt gattgctgct
    37381 cattttt
//
LOCUS       DBNCWK010000018        35510 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.018, whole genome shotgun sequence.
ACCESSION   DBNCWK010000018 DBNCWK010000000
VERSION     DBNCWK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35510
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.018"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            8..1885
                     /locus_tag="AC06IO_000799"
     CDS             8..1885
                     /locus_tag="AC06IO_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIO9874011.1"
                     /translation="MMNESSTEKKNELSLSFAALGVVFGDIGTSPLYAFGQVIKYFPI
                     NDHNIYGILSLIFWSLIIIVSIKYLVIVFRADNDGEGGIIALAGLIRQKIKKPGGWLL
                     FITLVGIGLIIGDGILTPAISILSAVEGLESLSPNLAKYVLPVTLIILFFLFKMQSIG
                     TGKIGVYFAPVMLIWFITIGVLGFLQITQNPKVLMAINPYYAIYFFMIHKYFALFILG
                     GVFLVMTGGEALFADLGHFGKKAIRTGWFAVALPALLLCYFGQGAFVLMHTEDIKYPF
                     FSLSPDWFLPVMIILATIATIIASQAIISAAFSILKQASLLNLIPRLKIIYTSKFEKG
                     EVYLPLINFILALGTCSLVVIFKSSSNLADAYGIAVNLDMLITTVLVGIIAYYCWNWH
                     AFKVMIFPLILVIELAFFAGNIPKLLTGGWIPILIAFLGFVVMYTWHCGFEKLRELHH
                     RDALMDAFIIDELNQNKISRQSGMGLYIIDPYDCEGESLLHHLRLNRIFFENMIFVSI
                     KIENKPYIPIEDKFELIKKAEGFYLIFIHYGFTENINLPNELDEMFKRVYLPFEIIKN
                     KLIYFIEIVFVEMTRERQKHMYMWQKHLFSLMIRNAVPDIQFYRLPYNKTIAIGTYYQ
                     L"
     gene            1936..2397
                     /locus_tag="AC06IO_000800"
     CDS             1936..2397
                     /locus_tag="AC06IO_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIO9874012.1"
                     /translation="MNKADLHNCVLFKGIDEKYIDDFLENCEEVELPEGEFLFHQNEI
                     GDSMYIVEQGELQIILDQNSTNSDPQEEQVIGVLKSGALFGELCVFGQLKRAASIRAL
                     VDCRLLKIEGEDFRIRIYSKDLDALLICYNIAKLLSQRFITLLSLRPSLNM"
     gene            complement(3452..4780)
                     /locus_tag="AC06IO_000801"
     CDS             complement(3452..4780)
                     /locus_tag="AC06IO_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HIO9874013.1"
                     /translation="MNCFRNLRLLFYLIYVGDLCANPLKLTSPLSHSLSREYQDTSSI
                     SSNPAAVNVRTGSGILQQYIEKKLGIQDSHGISTQFAWIGDTNELFGGGIPNAKDSTS
                     NSVGLLDLTIQMEHFNGWKGGLFSAQFLQQNAQNTNGPAGVIQGYNSLPDVPPYNRSE
                     LYALWYRQALFDDTFFIRLGKTITTLDFNNVIKPVQLSKDEPNIPAVTSLIYTPIFIN
                     PSVDGLMPGYTNTAYGITATYTPIKSWYASYGIYDGNLARGKQTGLSGPTFNGNYFHV
                     GETGSAWVLGKDKKPGTIGVGLWHQTGLINQENLTQRNPTGAYLFGSQRLWYRHPGQD
                     TSGISGFYQYGINNSRVLPMKQSIGAGLTAFGLIANRKADSIGSGFSLAWLNQRVTNR
                     KTELMFQIYYQAKLFNFIYLEPVLSYIPTPGQSSHLNPASAGTLRAIVLL"
     gene            4934..6136
                     /locus_tag="AC06IO_000802"
     CDS             4934..6136
                     /locus_tag="AC06IO_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIO9874014.1"
                     /translation="MLNKIRAFSLLIAIVFFSNVSAETYKVPATKDTVTLGLFTLDKT
                     PVVKVHSGDSVSLETWNSCLHEMVFNKTTPAEIAKFYTKHDIQKRRGMHSLTGPVYVE
                     GAEPGDVLEIRVLDIKLNDFGYNFLSPTQGILSEFTKPRIMYFKYDKEKNTTEFTKGI
                     CLHLKPFPGVIAVAPPLDWPDGWPVNLETQMGQPVPGQFNSVPPGPFGGNLDEPSLQV
                     GSILYLPVFQKGALVWTGDSHAMQSNGEIDVTALETSFEGITLQFIVRKDLKAEGVFD
                     KTKRNGNSLFTWPIVENSEEWIIIGLHENLFEAMQLASKNAIEFLVRTQGLSSQESYQ
                     FLSMVGNFDIPEAVNVIKSVAVHIPKEIFKNNGKMTSLSSEGKFPLTTPRINENATCM
                     PQEGKIIE"
     gene            6152..6553
                     /locus_tag="AC06IO_000803"
     CDS             6152..6553
                     /locus_tag="AC06IO_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874015.1"
                     /translation="MNKILIYLCLYFMTSITLAGKIVINNKTNYPVKDNSRKIAVQWV
                     TTVEATQKANKIIINGSKLDLSSLKIITQKGKTQLTLPNHSHYFRVVVWSTGKQQPDL
                     LTNWVDIVPNKTYIINQDQLVPAILMSGTGC"
     gene            complement(6825..7736)
                     /locus_tag="AC06IO_000804"
     CDS             complement(6825..7736)
                     /locus_tag="AC06IO_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIO9874016.1"
                     /translation="MTIQKGLHERRALKISIAVTFLLAVVGILFGLLSGSLAIVFDGM
                     FNMVDTVISIPAFFVARLLTSKGNRRFQYGYWHIEPMVLVLNGSILILLYTYALVNAI
                     GSLMSGGHELNFDWAFVFALLVFFLSTGMYFYLVKTNRKIKSEFLRLDIQSWLMSALI
                     STSLLLAFGIAALLHGGAYGYFTPYIDPLILAILTAFLIFVPMSAVRDAMRDIFRMAP
                     VGLDERIREFLDELIKQHDFKTYTSYVAKIGRAQFIEIHIVVPMDYPISSIETLDKIR
                     NEIALAIGEDTPQRWLTIAFTANENWI"
     gene            complement(7893..9299)
                     /gene="dbpA"
                     /locus_tag="AC06IO_000805"
     CDS             complement(7893..9299)
                     /gene="dbpA"
                     /locus_tag="AC06IO_000805"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HIO9874017.1"
                     /translation="MIQSEHTDQHLSFSQFPLRQELIKSLASSNYENMTPIQMQSLPI
                     ILRNEDLIAQAKTGSGKTAAFALSLLNNLKISFFAVQALVLCPTRELAEQVSQAIRRL
                     ACFMPNVKIINLSGGIPMQPQLDSLRHGAHIIVGTPGRILKHLKNASLDLSQVKALVL
                     DEADRMLDMGFFDDIKNIISVCPKQRQTLLFSATYPEEIKQLSKQFMKNPKEVYVETP
                     LDEIDIEQHFYEVTKQTQKFPLLKSLLLHYQPVSVLIFCNTKQQTIEVTDQLINEGFS
                     AIALNGDMEQIERDLAVLRFANQSCSILVATDVAARGLDIKELSAVINFDLAFDHDVH
                     IHRIGRTGRAGSKGIALSIVTPADAQRICAIEDNYPHPIHWGNINELENHNTARLVPE
                     MVTLCIASGKKDKIRPGDILGALTKDAGLAGNTIGKINITAMYSYVAIHRSQADKAYQ
                     YLQSGKLKGRKVNVRKIN"
     gene            complement(9540..9749)
                     /locus_tag="AC06IO_000806"
     CDS             complement(9540..9749)
                     /locus_tag="AC06IO_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIO9874018.1"
                     /translation="MSKTVNGVVKWFNELKGFGFIEQEGGPDVFAHFKEISSSGFKTL
                     TEGQRVQFIVTQGAKGLQAQNITPL"
     gene            10411..11196
                     /locus_tag="AC06IO_000807"
     CDS             10411..11196
                     /locus_tag="AC06IO_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HIO9874019.1"
                     /translation="MSSKINWLLNNIASGEILLQSWLSSHGITPQLARKYCQSQWLVK
                     LGVGVYYRAGRKPRWQNAVYCLQNQSRYMIHLAGLTSLAQQGKSHYLQFAPDSIWINV
                     QAKTILPKWFKNFPELVISDELIPNWEIIKTSKLNAQDENDLTTIEVDGIKLKASNQE
                     LAAFEVLEAVPSTLSFEHAAELFQGLVNLSPRKVEHILKRSGSIQTNRLFLFLGHFYK
                     HPWAERVNESLINLGAGKRQIVPDGKLEKQYQITVPEQFISSD"
     gene            11200..12132
                     /locus_tag="AC06IO_000808"
     CDS             11200..12132
                     /locus_tag="AC06IO_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIO9874020.1"
                     /translation="MNKQSPYYKQVLLLIRLIPLVEKETCFALKGGTAINLFVRDFPR
                     LSVDIDLAYLPLEPREIALANVRNALAGIAANINKIPDLNAVLQDNKPDEMRIIVEDH
                     SSLNRGTQIKIEVSPVARGTLLPPVELDVVASVENEFGFASMKVVSLPDLYGGKLCAA
                     LDRQHPRDLFDVKVLLENQKIDRELFNGFLTYLLSHKRPISEIMNPRWKDITQVFERE
                     FKGMTAEPVTLEELTAIPGKMIQGLKDNFTQQDYDFLVSFKRGEPEWSLACSDQIQYL
                     PAVQWKLLNIRKMPKQKLMDSVSLLQRTMGHWLN"
     gene            complement(12265..14205)
                     /locus_tag="AC06IO_000809"
     CDS             complement(12265..14205)
                     /locus_tag="AC06IO_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HIO9874021.1"
                     /translation="MATLLTKSKFKMALECPTKLWYYDRPDEYTNILDEDSFLKALAE
                     GGAQVEALARCYFPDGISIKSSSTTEALNKTQELIQRDSITVFEASFQYQNYFLRADI
                     LQKRPGHIKIIEVKAKSISKKDESKMATKKWQPYIADIAFQKWVLEQLYPSFTISSYL
                     MLVDKDAICPTDGLNQKFLVQKDSKGKAIVTLTSPITEEDLSIKILREINVDHHIELF
                     WQEKNDEEQTVKDQFHNFSKIYFSGQRFPPKAKSECGDCQFNTNESDKFLSGFKECWR
                     ESLGYKEADFNDPTILDLWDCRDKPKYFQQGLIKLKDINETAFKINESDKSGISRTER
                     QWLQIEKIKTQDNSVWIDKENLKTEIDSWRYPLHFIDFETAMLPIPFNKGSHPYQGIA
                     FQFSHHTLNENGQVAHVGQYLNSEPGVDPSIEFVRYLKKELENDDGTIFRYSNHENTY
                     LNSILERLEQMIEPPNDKEDLCSFIRSITKSPSDSKVKWEGPRCMVDILELVKRFYYD
                     PLTNGSNSIKQVFPAILNRSEFLQEKYNQPIYGTVSGIQSHNFINQCWIVKENNRIKD
                     PYHLLPPINKDISDEDAPLLFDSEDLKEGGAATIAYAKLQFTNMSDSEREELRNALLR
                     YCELDTLAMVMIIEAWQHMLQI"
     gene            complement(14320..14757)
                     /locus_tag="AC06IO_000810"
     CDS             complement(14320..14757)
                     /locus_tag="AC06IO_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HIO9874022.1"
                     /translation="MSEKLDKIIQDITVKHGVLLGKDDPILMLQTMNAQLIEDNRKAQ
                     QDLLVQFREEMEGISSQWKDDAKEKAEKVLNAALASSKEAITRLLHESTKESVQAMKK
                     LISDSLIEAHSFTQKTQKFSRFALVSSATLFAASCIVLLLFCK"
     gene            complement(14757..15482)
                     /locus_tag="AC06IO_000811"
     CDS             complement(14757..15482)
                     /locus_tag="AC06IO_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HIO9874023.1"
                     /translation="MAKIHITLQGKGGVGKSFISATTAQYKQHKGQPPLCIDTDPINS
                     TFHGFKALNVDHIQVMSGDEINPRLFDALIEKIASTTNDVVIDNGASSFVPLSHYLIS
                     NQVPALLQELGHEMVIHTVITGGQALFDTINGFAQLIDQFANEAQFVVWLNPYWGAIE
                     HEGKAFEQLKAYRDNKDRISALIHIPDLKKETYGRDLTEMLQQKLTFDEVLGTPEKSI
                     MTRQRLKIVRDQLFGQLDGARVI"
     gene            complement(15482..15826)
                     /locus_tag="AC06IO_000812"
     CDS             complement(15482..15826)
                     /locus_tag="AC06IO_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HIO9874024.1"
                     /translation="MENSLSERIAAKQMVRKASAKSVNKAAFLALKNDIASALADGWS
                     IKLVWETLVEEGKISFSYKTFCGYVARLIAAEKKPSMQENTKEDEQAKSKTKTEIRGF
                     TFNPKPNLEELL"
     gene            16220..16573
                     /gene="traJ"
                     /locus_tag="AC06IO_000813"
     CDS             16220..16573
                     /gene="traJ"
                     /locus_tag="AC06IO_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HIO9874025.1"
                     /translation="MDDKLKTPTRKNGRHLRVPVLPSEEIQIKSNAATAGLSIAEYLR
                     RISLGYQIQSSVDKDYVLELAKINGDLGRLAGLLKLWLTQDKRVAHFDLHTVKTLLNR
                     IQATQDAMLEVVKKL"
     gene            16570..18441
                     /gene="traI"
                     /locus_tag="AC06IO_000814"
     CDS             16570..18441
                     /gene="traI"
                     /locus_tag="AC06IO_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="HIO9874026.1"
                     /translation="MIIRHIPMKKTRLSSFSGLVQYLCNQQNKQERVGKVRLSNCNSL
                     DPIWAVQEVLATQAKNQRATGDKTYHMLISFAPGENPSPQVLREIEDRVALSIGFKEH
                     QRISVVHHDTDNLHIHVAINKIHPQAFNMIEPYRAYKIFAQAASQLEIELGLQITNHQ
                     TRKNRSENLADDMEHHSGIESLINWMKRHCKEKIEAARSWKEIHSILAEHGLMIRVKA
                     NGFVFCNPQGMTVKASSISRTFSKKNLESKLGPFVPSYSEGDAPESNIYRYEPLNKKI
                     LGSALYATYKHEKSHNKHIISDKLKNLREARAKLIEKAKKRGRIKRATLKLMNLSKVQ
                     KKFLYSHINKTLLNEIDSIRKNYAKERSRLLDSHQNKTWADWLKQKAQEGDQEALVAM
                     RYRNRKNNYDYTISGKDLVSTIFNFRQIDSVTKEGTEIYKKDMSVIRDNGKEIFISKG
                     GSISALKRALEMARQRYGDCICVNGSPLFKKIILQIVIQYQMPITFADLDLENQRQKL
                     NFEQEKSHEQSRSSGQNHRGRTPGSYETIGAANENRKKRGTKPNTFSIRQGSPAKDHN
                     GLRNLSQCDLVQFTGGGQVLLPDNAHDYLERKRFKLNNHVRRKIFGINEKRKNRKNK"
     gene            18548..19318
                     /locus_tag="AC06IO_000815"
     CDS             18548..19318
                     /locus_tag="AC06IO_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874027.1"
                     /translation="MDDNKLILDAANSYGFNLEFRAKKILEEKSFSVLMNQLIKNGDE
                     FVEIDIKAAQYQGREWLIECKGAANSSYLILIKEDNSNDPKLYNTRRHVIKDSNYRVA
                     QFKPDENQCFFTFTGDFFNKTGNQLKKASKNDFENNFFKAQNQILSAIKAISITDVDK
                     NKSRDFPIIIPVIITNAKLWVIDYNNSAAPEVSQYKWALHKIPTKNNLCIAPKNERKF
                     DSISILVLNIEHLEEFLSTYSFCEDGEISVGNGFICNR"
     gene            complement(19362..21548)
                     /locus_tag="AC06IO_000816"
     CDS             complement(19362..21548)
                     /locus_tag="AC06IO_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HIO9874028.1"
                     /translation="MTKMPYHEVVANQIIESLKAGTAPWLKPWEPGVGNGQIPYNPVT
                     GKRYRGINALYLMVNQSNDNRWLTYKQAQSMDAQVRKGEKGTTIQYWKFHEEQTKHDD
                     TGKPVLDEQGNPLKVQVNLERPKVFYATVFHASQIDNMPELIAKEPDWSLIERAEKLL
                     HNSGANIFHSEADRAFYRLSTDSIHLPPKEQFKSAANYYATALHELGHWSGHPSRLDR
                     DLGHPFGSDAYAKEELRAEIASMLLGAELGIGHDPSQHTAYIKSWIRVLEDEPLEIFR
                     ASADAEKIVNHLCALEQTQNLQLSELIEIKDDKLKEEVYMATDNITSEKVWLSIPFKQ
                     KEIAKSTIGKLPDGTPGIVWDKVQKCWYANPGVPVEKIKAWLPEHQVLTQEKALIPED
                     EFKAALISLGAIVAGGHPIMDGKPHRIPTEGDKNGEKAGFYVAHLDGIPAGYIKNNRT
                     GAELNWKCKGYILTGEQKAALKAGALEHQQTRQRELEVKQKSTALRLQQKLSMMTEVV
                     EPTPYMKSKGIQVHSGVYTDEEKKLTCIPATDCEGTIWTVQYISEDGTKRFAKDSKKE
                     GCFHVLGSLEKLANAPVIIIAEGYATAATIKEACELPAVVSAFDSGNLKAVAKELNKK
                     YPHKPIILAADDDKYLELSKGINPGKEKASEAAEAVNGFIVLPTFASSEQSSNPKQFS
                     DFNDLANHSKLGFEGVIRQIKPTVEQIAKRYNRMRWSKNSNVIHIS"
     gene            complement(21541..21705)
                     /locus_tag="AC06IO_000817"
     CDS             complement(21541..21705)
                     /locus_tag="AC06IO_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874029.1"
                     /translation="MNHETELMDVIAEKFEDLVIPGFLLEVSPIEADSMGAFVEDALN
                     EKDAMEAIYD"
     gene            complement(21702..22247)
                     /gene="traF"
                     /locus_tag="AC06IO_000818"
     CDS             complement(21702..22247)
                     /gene="traF"
                     /locus_tag="AC06IO_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HIO9874030.1"
                     /translation="MKKLSGIISMFLISLIVVGALFHGMGFRINLTESIPIGLYRIDS
                     KAPIKNSYVIFCPDDRESFRLARDRGYIDHGLYCDGYGYLMKKVVAVSGDILSVTNKG
                     VFVNHILTPYSKPKIQDGMKRALPQWQVMNYQLQKDEVMTMTSQSEWSFDGRYYGLVH
                     AGQIKGMITPIWVINKRERTT"
     gene            complement(22244..24133)
                     /locus_tag="AC06IO_000819"
     CDS             complement(22244..24133)
                     /locus_tag="AC06IO_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HIO9874031.1"
                     /translation="MSQNKPNKAIGPQVREKNHGKINRTLILLVICSFLISMHIGTQF
                     FAHQFHYSDALGGTFAPIYMPWQIFIWAIKYQPYYPDSFNAAFGLIVMMGCLFLMIIV
                     LASKYFKKNNVSDYLHGSARWANWEDLNSASLIGHEEGVYVGAFENDKGAIHYLRHNG
                     PEHVLTYAPTRSGKGVGLVIPTLLSWKHSCVITDLKGELWAMTAGWRQKHATNKVIRF
                     EPATLKGSARWNPLDEIRVGTESEVGDVQNLATLVVDPDGKGLETHWQKTSQALLVGF
                     ILHAIYKLKTQGEPATFPNIDRMLVDPNINIADLLIEMTQYPHVEGKTHPVISASARD
                     MIDRPEEEAGSVLSTLKSYLALYRDPVVAHNVSASDFCIRDLMNHECPVSLYIVTQPN
                     DKARLQPLVRVMINMIVRLLADRMEFERVSDDRGLSYVSAKKTYKHRLLCMIDEFPSL
                     GKLDILQESLAFVAGYGLKFYLICQDINQLKSRERGYGPDETITSNCHIQNAYPPNRV
                     ETAEHLSKLTGQTTIVKEHITTSGKRFSTFLNQISKTVQEISRPLLTVDECLRMPGPK
                     KDANGLIVEAGDMVVYAAGFPAIYGKQPLYFKDPIFMARASVEAPVHSDILRTRLSDD
                     EVIRL"
     gene            complement(24130..25512)
                     /gene="trbL"
                     /locus_tag="AC06IO_000820"
     CDS             complement(24130..25512)
                     /gene="trbL"
                     /locus_tag="AC06IO_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HIO9874032.1"
                     /translation="MNKKIFPYVIAFGLFGFSACCHAKGHGMDSRDLLDNILQRFAST
                     SALWSKTMLSYARYLFWSLASISMVWTYGLMALRKADIQEFLAETVRFFVVVGFFYWI
                     LDNGPAIAKAIIDSMRQLAAKASGINEHVSPSDIVDVGFDIVSKAIDSSSIWSPAATT
                     VGLLVAGVILIILALVSINMLIILITAWILTYGGIVLLGFGGGRWTQDIAINYYKTVL
                     GIALQAFAMILIIGIGKSFVDQYYAAMSENIMLKEMFVMLVVAVLLLVLIDKIPPALA
                     GIVSGGGSGGGGGLGLGGAMGAAGIAGVAMAGAAGSVLAQSGGGLSALNAAFKAASQS
                     TNSGDTNALSGMDKSSKTGGLAEAMGSAAKFAGSFGSHLASGTMDVAREKANSIKEAV
                     AEKIADTTGGKIAEAIQNKVDTSVCESQESNAKNILSMGTPSGSLSSGFEASDEISQF
                     VNQKASGESQ"
     gene            complement(25517..25729)
                     /locus_tag="AC06IO_000821"
     CDS             complement(25517..25729)
                     /locus_tag="AC06IO_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874033.1"
                     /translation="MKILCLSIMVVACVLSGCDSAESKAQKKAEHLASLTCSSPREGR
                     TQEELQAIGDACFRGGSYSKSSGKKW"
     gene            complement(25753..26493)
                     /gene="trbJ"
                     /locus_tag="AC06IO_000822"
     CDS             complement(25753..26493)
                     /gene="trbJ"
                     /locus_tag="AC06IO_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HIO9874034.1"
                     /translation="MKFKSMIIYCFVLPVFASGAPVFDVANWIQNGKMIATQISEYKT
                     QVDQYKNQMDQYQNMLENTKSLTSFEWDNANSVINNLLESTDTIDYYKQEAGSLQGYL
                     DRFQSQEYYQKTPCFNGNGQCSAEELKKIKQSRLAASVAEKRSNDAMLKGIDKQQQSL
                     KNDSAKLRTLQSQAQNAAGQKQALQAASQLASNQSHQLLQIRGVLLAQQNAQAVKDAA
                     SANKEAIQTAGDEHFRSGAYHKSSGKKW"
     gene            complement(26505..27752)
                     /locus_tag="AC06IO_000823"
     CDS             complement(26505..27752)
                     /locus_tag="AC06IO_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HIO9874035.1"
                     /translation="MNQNNDYLSPDNSPQKLQTTGVKRVNNVPLIITIGILTLFVVLI
                     ALVANKRANAQNQPPEIVKVKNPKKNTMSLANDVVGNHKSAVISPQSLTIANSETTPL
                     PIPEQLPHKEDTLTQDVSEAEIERIRQEKTQEFEEAVKAKTSVMVDNPRLNNRDTTKT
                     SMNSNEMALNIDPSLAFKEQLALLQGRQAKPMPQTLGGEENEMRWHLNSTLQNPNSRY
                     ELRAGSVIPGVMISGISSELPGQIIAQVSQNVYDTATGKYLLIPQGTKILGLYSSDVP
                     FGQNSVLVAWQRLVFPDSKALDIGSMPGADSAGYAGFRDQVDHHYARIYGSALLMSGI
                     IAGITYSQNTNQSNQFGYAQPTAGSVMSQALGQQLGEVTSQLVSKNLNVSPTINVRPG
                     YRFNIIVVKDLTFKQPYKQFAYQ"
     gene            complement(27758..28165)
                     /locus_tag="AC06IO_000824"
     CDS             complement(27758..28165)
                     /locus_tag="AC06IO_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbH"
                     /protein_id="HIO9874036.1"
                     /translation="MKKLSVLLAAVLLSSCASMRYGNFTQMSQGKDAYLAADAATQLT
                     RVYPPAQNTFCIGQRVTDGFGLSLIQNLRKKGYGINENQCPGNKANFFYVLDELKPQS
                     YRVSLFVGIQTLSRVYAKRHENIIPASTWTHKE"
     gene            complement(28168..29043)
                     /gene="trbG"
                     /locus_tag="AC06IO_000825"
     CDS             complement(28168..29043)
                     /gene="trbG"
                     /locus_tag="AC06IO_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HIO9874037.1"
                     /translation="MKRIMQLFCLLTPLTSFALDNTILAEHYFSDTVPKLSSQEKQGL
                     DIAERTQQGEKTSIPFASSDGSVSFIYGSGQIRVVCAPLQVCDIALQPGEQFNDMNVG
                     DPRFVVEPSLTGSGAMQQIHLLIKPKDVGLDSSLVVTTDRRTYHFRLKSDRTEFMPYI
                     SFTYPDEAKAKWQLLQRIQAERRKVDTLPETNEYLGDLNFNYRIQGNARFKPVRVYNN
                     GVKTIIEMPRTMAQSEAPALLVLRSLGLFKKSESVMVNYRLQGCRYIVDGVFDKAILV
                     IGSGSSQEKITITRC"
     gene            complement(29040..29738)
                     /locus_tag="AC06IO_000826"
     CDS             complement(29040..29738)
                     /locus_tag="AC06IO_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HIO9874038.1"
                     /translation="MKKSITNNPYLNARRAWNVHTAGLMKSLQVWQLVGLSSLLIALA
                     AVGGLIVIGSQSKFIPLVFQQDANGNTLSVTRADSVGEASIDDYRAAAAHFIENIRMV
                     SLDVELQKKAVFQVYSYLNTNDAALAKVQEFYSDKQQSNPFERAAFEIVSIDIRSVLQ
                     ESNETWQVDWIETVRNRDGSLKEKPRMMKAMITLYQENEINDATSESILKNPHLIYVR
                     DFNWSYELKHGEQQ"
     gene            complement(29735..32269)
                     /locus_tag="AC06IO_000827"
     CDS             complement(29735..32269)
                     /locus_tag="AC06IO_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869966.1"
                     /note="type IV secretion system ATPase VirB4 family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HIO9874039.1"
                     /translation="MIGFITFLISITGLLLLGTLFSAAQMKSRKHHVRRHRSHTAGLV
                     DLLNYAAVVDDGVIVGKNGSFMAAWVYQGEDIANTTDEAREMMAFRINQAMSSLGNGW
                     MIHVDAIRRAAPGYSEQSASYFPDPISKAVDEERRQYFEGIGALYEGYFIITLTWYPP
                     VLAQKRFVELMFDDSGEKLNHKAKTHQLIETFKQSCRNFESRMSAALHLERLGSELVI
                     NENNKVTQDNFLRHIQYCVTGINHPVNLPKNPIYMDALIGGQELTPGITPKIGRKFIQ
                     CVAIEGFPMESYPGILTLLTQLPVEYRWNSRFIFMDPHEAVAHFTKFRKKWKQKVRGF
                     FDQMFNTNSGVVDEDALGMVNDAQAAIAETNSGMVGQGYYTSVVVLMDEDRANVEKAA
                     LFIEKNINALGFSARTETINTMDAYMGSLPGHGVENIRRPLINTMNLADLLPTSSIWT
                     GENKAPCPLFSLHSPPLMHCVTSGNAPFRLNLHVRDLGHGIMFGPTRSGKSTHLGLLA
                     LSWRRYKDARIYSFDKGMSMYPTCKATGGEHFTIADKDSRLAFAPLQFLETKADRAWA
                     MEWIDTILALNGLNTTPVQRNEIGHAILSMHQSGSKTVSEFVMTIQDEPIRETLKQYT
                     IDGLMGHLVDAESDGLGLSSFMTFEIEHLMCLGEKFALPVLLYLFRRIETSLDGRPTL
                     ILLDEAWLMLAHPVFKNKIAEWLDSMAKKNCVVFMATQHLSHAANSGILDIIVESTAT
                     KIFLPNLYARDPETQTIYERMGLNPRQIDIIAAAQPKRDYYYVSEKGQRLYQLALGPY
                     ALAFVGATDPDSIARMKQLEFKHGDAWVSQWLLEKGIRMNQYGEAA"
     gene            complement(32266..32565)
                     /locus_tag="AC06IO_000828"
     CDS             complement(32266..32565)
                     /locus_tag="AC06IO_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HIO9874040.1"
                     /translation="MSLRTIPIRRCGNRPSLFMGGDRELVMFSGLLSAILVFAAQDWL
                     AAIFGFALWFLSLKGLRLMAKADPYMRAVYLRQRGYQAYYPARSTPFRNNKGGYL"
     gene            complement(32562..32939)
                     /locus_tag="AC06IO_000829"
     CDS             complement(32562..32939)
                     /locus_tag="AC06IO_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HIO9874041.1"
                     /translation="MNQAVNINYRSIWMMGAIVLLLLFITDPACASSAGGGLPFDSYL
                     TKIQKSITGPFAFTAAIIGLVGAGATLIFGGEMNGFLRTLLFIVLVLSFLVAAQNTMT
                     AITGKGAEIAKPSVLTSAMESQL"
     gene            complement(32951..33916)
                     /gene="trbB"
                     /locus_tag="AC06IO_000830"
     CDS             complement(32951..33916)
                     /gene="trbB"
                     /locus_tag="AC06IO_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HIO9874042.1"
                     /translation="MKAQEEQGITVKDRAREKLRRDLGGMIEQALSDPKTVEIMLNAD
                     GKLWQERLGETMRCIGSITDARAESIIKTVAGFHGKEVTRFNPILEGELPLDGSRFAG
                     QLPPVVSKPTFAIRKKAISVFTLDDYVKSGIMKEAQCAVIKKAVIAHRNILVIGGTGS
                     GKTTLVNAIINEMVRSSPAERVFIIEDTGEIQCAAENCVQYHTTLDVNMTQLLKTTLR
                     MRPDRILVGEVRGSEALDLLDAWNTGHEGGAATLHANNCIAGLHRLKSLITRNPSAPA
                     EIEPLIGETVHCVVHIARTPQGRRVEEIISVKGYENGHYNIEKLI"
     gene            complement(33944..34204)
                     /gene="csrA"
                     /locus_tag="AC06IO_000831"
     CDS             complement(33944..34204)
                     /gene="csrA"
                     /locus_tag="AC06IO_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIO9874043.1"
                     /translation="MLVLTRKVGESVVISEEVYCTVVGYRDGEVRLAFDAPQSIPVHR
                     DEIQRRIYRERQKDQWFSDSPSNKESIIDRLISKFKHGLKSA"
     gene            complement(34221..34532)
                     /locus_tag="AC06IO_000832"
     CDS             complement(34221..34532)
                     /locus_tag="AC06IO_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HIO9874044.1"
                     /translation="MFTRFAVFYGHLWRSQFKSDGFLEFAKKEWLEGLNQFSDEILNQ
                     VIIDCRDHCEMPPTLPQMIGFCRDIKRRNAFYVASEKYQPARKEVVEENIRQCRAYLF
                     K"
     gene            complement(34552..35436)
                     /locus_tag="AC06IO_000833"
     CDS             complement(34552..35436)
                     /locus_tag="AC06IO_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874045.1"
                     /translation="MYFTVNDKELDALCGLSYIQQITYLRGIRPYMDRNTFIVGIKRR
                     ISYQSLAEVLYIEPHQGIKESGSPSRQQIRRAIKGLERSGLIAIQSFDKHLILKCLLA
                     DISYCVQNKPDTNPTQQADTKPHAVFPVNTRVSEDTERKADTDKILKADTPQIKENNY
                     IYLLRYFDHFWHMYPEKKSRERAFEIFKQINPDEPLLQCMLQALAQQIKARTAKEALG
                     EWVPAWKFPANWLSQKCWEDEVKIELTQEKRNEKCGTNTKSRAIDPFWNPETGDAKIT
                     GEDEYQQSNVVNLQCYRQ"
BASE COUNT        10527 a         6932 c         6902 g        11149 t
ORIGIN      
        1 accccggatg atgaatgagt cttcaacaga gaagaagaac gagctcagct tatcatttgc
       61 ggctctgggg gttgtttttg gtgatatagg aacaagtcct ctttatgcat ttggtcaagt
      121 aataaagtat tttccaatca atgaccataa tatttatggt attttatcgc tcattttttg
      181 gtcgctgatc attatagtca gtattaagta tctggtaatc gtgtttcgtg ctgataatga
      241 tggcgaaggc ggcattatag ctttagcagg gttgataaga cagaaaataa aaaaaccagg
      301 aggctggctt ttatttatta ctcttgttgg catagggctt atcattggtg atggaatatt
      361 aactccagcg atatccatat taagtgctgt tgaaggtctg gaatctttgt ctcccaattt
      421 ggccaaatat gttcttccag tgacattaat tattttattt ttcctcttta agatgcagag
      481 cataggtact gggaaaattg gtgtttattt cgctcctgtt atgcttattt ggtttattac
      541 cataggcgtt ttgggttttt tacaaattac acagaatccc aaagtactga tggcaataaa
      601 tccttactat gcgatttatt tttttatgat acataaatac tttgctcttt ttatattggg
      661 aggagtgttt ttagtaatga ctggtggtga ggctttattt gctgatttag ggcattttgg
      721 caaaaaagcc attcgaacag ggtggtttgc tgtggcgctt cccgcattac ttttatgcta
      781 ttttggccag ggagcgtttg tattgatgca tacagaggat atcaaatatc ccttttttag
      841 tttatcgcca gattggtttc tccctgtgat gattattttg gctaccattg caaccattat
      901 tgcatcacag gcaattattt cagcggcatt ttcgatttta aagcaggcgt cactgcttaa
      961 tctgattcca cgtcttaaga ttatctatac gtccaaattt gagaaaggcg aagtatactt
     1021 gccacttatc aattttattt tagctttagg tacatgttca cttgttgtga tttttaaatc
     1081 atcatctaat cttgccgatg cttacggcat cgctgttaat ttagatatgc ttatcacaac
     1141 agttttagtt gggatcattg cttattattg ctggaattgg catgcgttca aggtaatgat
     1201 tttccctcta atattggtta ttgaacttgc attctttgct ggaaatatac ccaagttgtt
     1261 aactgggggt tggatcccta ttttaatcgc gtttctagga tttgtagtga tgtatacctg
     1321 gcactgtggc tttgagaaac tacgggaatt gcatcataga gatgctctga tggatgcttt
     1381 tatcattgat gaattaaatc aaaataaaat ttcaagacaa tccggaatgg gattatatat
     1441 tatcgatccc tatgattgtg aaggggaaag ccttcttcat catcttcgcc tcaaccgtat
     1501 tttttttgag aatatgattt ttgtaagtat caaaattgaa aataaacctt atatcccaat
     1561 tgaagataaa tttgagctta taaagaaagc tgaagggttt tatttaattt ttattcatta
     1621 tgggtttact gaaaatatta atttgcctaa tgaattagac gaaatgttta aaagagtata
     1681 cttacctttt gagataatta agaataaatt aatctatttt attgagatcg tttttgttga
     1741 aatgactaga gaaaggcaaa aacacatgta catgtggcaa aagcaccttt tttctctcat
     1801 gatacgcaat gctgtgccag atattcagtt ttatcgatta ccttacaata aaactattgc
     1861 aatagggact tattatcaat tatgagccca ttcaaagagg cgatattggg tttttaagtg
     1921 gttaaggata gtgaaatgaa caaagccgat ttacataact gcgtgttatt taagggaata
     1981 gatgaaaagt acattgatga ttttttggaa aattgtgagg aagttgaact tccggaaggt
     2041 gagtttcttt ttcatcaaaa cgaaattggt gactcgatgt atattgtcga acagggtgaa
     2101 ttacaaatta tactggatca aaattcgaca aactcggatc ctcaggagga gcaagtgatt
     2161 ggagtactta aaagcggagc attgtttggg gaattatgtg tatttggaca actaaaacga
     2221 gccgcatcaa tccgcgccct ggtagattgt agattattaa aaatcgaagg ggaggatttc
     2281 aggattcgta tctactcaaa agatttggac gcattgttga tttgttataa tatcgcaaaa
     2341 ctattaagcc agcgctttat tactctttta agcttgcgtc cttctctgaa tatgtaattt
     2401 cagtagatag agaatagcta aaccccgaat tatgttctag cagttttctt gcttatggat
     2461 tcaagtgatt tttttattct tgccctgctg tagttatttg attttcaata acttccaaga
     2521 caacgtagtt gcctcagctt gtccctaatg gttccagtac tacttttttc aaacgtatga
     2581 tgttttcatt attaatatga cgctgttaac aaagtatggt gaggtagtat aaagagcatt
     2641 ttattaagag cacaggaatg aacgcaaggc tttacccgct aaaagaagag gattttataa
     2701 gagatatagc acccctatat aaccacgccg tggatgtttg gttggattga gccattatca
     2761 gtttttttgt gcggtgttct ttgtattaag accgtcagtt ggtgagatgt tcctgctgct
     2821 atggaaaatg gcataccatt tatacacgat ttaaacgatg gagtgaaaat ggattgtctt
     2881 ggtttttggt ttgtcaactt caacaaagga aaaagataac tgtgtatatt agctgggtag
     2941 atagcacgac tactggtatt tatcactata gctcaggcac attaaaaaaa gagaccgcaa
     3001 gttataggcc ggggccgcaa aggagtggga accaaaattc acgtggcgct ttctgctgag
     3061 aaaattcaag gagcctgttt atcagtaact aacatcgttg atatgaagag tttactgtgc
     3121 tttgcaagaa atgtgattgg cgaggtatca actataatgt tgctgattaa gtccttttat
     3181 tgttgagcta gcttttccta tactttcttt cagatgggaa gcttatgttg tcaattattt
     3241 gatatcttta cgtttaaacg aataggccat catcgcacgc gattttagta cctgtacttt
     3301 aaatcagaaa tctctctatt cgctgctgtg gaacacttta agtgctttag ttaaaagtac
     3361 cgaccccaaa ctaaacaaac aaaaatggat aaactgccat caaaatcaat tggtagatag
     3421 gttaatcaaa gagaacacac aatttgacag tttaaagtaa cactatggct cttaaggtcc
     3481 cagctgatgc tggattgaga tgactacttt gccctggagt agggatataa gagagtactg
     3541 gctcaagata aataaaatta aataattttg cctggtaata aatctgaaac attagctctg
     3601 tttttctatt ggtaactcgc tgatttagcc aagccaaaga aaacccggat cctattgagt
     3661 ctgcttttct attagcaatt aatccgaatg cagttaaacc tgcgccaata gattgtttca
     3721 taggtaatac tctagagtta ttgatcccat attgataaaa accagaaatc ccgcttgtat
     3781 cttgaccagg atgtctatac cataatcgtt gagagccgaa taaataagcc ccagtaggat
     3841 ttctttgagt caaattctcc tgattaataa gaccagtttg gtgccataat ccaactccaa
     3901 tggttcctgg ttttttatct ttacctaaaa cccaggcgct ccctgtttca cccacgtgaa
     3961 agtaatttcc attaaaggtt ggtcccgata atcctgtttg ctttcccctt gctaaattac
     4021 catcgtaaat accataagac gcataccatg atttaattgg cgtataggtt gctgtaatac
     4081 cataagccgt attggtatac cccggcataa ggccatctac agaagggttt ataaatatgg
     4141 gtgtataaat taaacttgtt acagcaggaa tgttaggctc atctttgctt aactggacgg
     4201 gcttaatcac gttgttaaaa tctaaagtag ttatagtttt accaagccta atgaagaaag
     4261 tatcatcaaa caaagcttgt ctataccaaa gagcatagag ctctgaacgg ttatacggtg
     4321 gaacatcggg tagcgagttg tacccttgta tcacacctgc agggccattt gtattttgcg
     4381 cattttgttg gagaaattgt gcgctgaaaa gcccaccttt ccagccattg aagtgctcca
     4441 tctgaatggt taaatccaaa agccctacgc tatttgaggt gctatctttt gcattgggta
     4501 ttcctccgcc gaacagctca ttagtatccc caatccaagc aaattgagtt gaaataccat
     4561 ggctatcctg aatccctaat ttcttttcaa tgtattgttg taatattcct gagccagttc
     4621 ggacattaac tgcggcgggg tttgaactaa tgcttgaagt atcttggtat tctctactca
     4681 atgaatgact taatggactt gttagtttta aaggatttgc acaaaggtct cctacatata
     4741 ttaaatagaa caacaatctt aaattacgaa aacaattcat aatagttctt gtgatgaatc
     4801 tttaagatac tgtatcacaa taaattttat actcaacgga cttgaatttt tatgcaattc
     4861 atactaatca taataggtta tttgatgcaa actatggcaa acttccttca ttagccattc
     4921 ttaaggacta gaaatgctaa ataaaatccg agctttctct ttattaatag caatagtatt
     4981 tttctcaaat gtttcggcag aaacatacaa agtcccagct acaaaagaca cggtaacact
     5041 cggtcttttt acattggata aaacacctgt agttaaagtt cactctggag attctgtgtc
     5101 cttggaaacc tggaatagtt gtctgcatga aatggtcttt aataaaacaa cgccagctga
     5161 gattgcaaaa ttctatacaa aacatgacat tcaaaaaaga cgtggtatgc atagcttaac
     5221 tgggcctgtt tatgttgaag gagccgagcc tggggatgta cttgagattc gggtattaga
     5281 tatcaagtta aatgattttg ggtataattt tttaagccct acacaaggga ttttatcaga
     5341 gtttaccaaa ccaagaatca tgtatttcaa atatgataaa gaaaaaaaca ccacagaatt
     5401 caccaagggc atatgcttac acctaaagcc ctttccggga gttatcgcag tagctccacc
     5461 tctagattgg ccggatggat ggcccgtgaa tttagaaact cagatgggtc aacctgttcc
     5521 agggcaattc aattcagtcc cgccaggtcc ttttggaggg aacctggatg aaccaagctt
     5581 gcaagtaggc tccatccttt atttgcccgt ttttcaaaaa ggtgcgttag tttggacagg
     5641 agattcacat gccatgcaaa gtaacggcga aattgatgtc acagcactag agacctcctt
     5701 tgaagggatc accttgcaat ttattgtaag aaaagatcta aaagctgaag gggtatttga
     5761 caagaccaag cgaaatggaa acagtttatt cacatggccc attgtcgaaa attctgagga
     5821 gtggattata ataggtttac atgaaaattt gtttgaagct atgcagcttg cctctaaaaa
     5881 tgcaattgaa tttttagtac gtacccaggg actatcttcg caagaaagtt atcaattttt
     5941 gagtatggta ggtaattttg atattcctga agcagtcaat gttattaaaa gcgtagccgt
     6001 tcacattcct aaagaaatct tcaaaaacaa tgggaagatg acttcgctta gctctgaagg
     6061 taagttccct ctaacaacgc caaggattaa tgaaaatgca acctgtatgc ctcaagaagg
     6121 caaaattatt gaataacctt taggtgaaat aatgaataaa attttaattt atttatgctt
     6181 atattttatg acttcaatta ccttagctgg caaaattgta attaataata aaacgaatta
     6241 ccctgtaaaa gataactcca gaaaaattgc cgtacaatgg gtaacaaccg tagaggctac
     6301 tcaaaaagcc aataaaatta taattaatgg ttcgaaattg gacctaagct ccttaaagat
     6361 aatcactcaa aaagggaaaa ctcagctcac tctgcctaat catagtcatt attttagagt
     6421 ggtggtttgg tcaactggca aacagcaacc ggatttactt acaaattggg tagatattgt
     6481 tcctaataaa acttatataa taaaccaaga ccaacttgtg cctgcgattc taatgtctgg
     6541 gacaggctgc taaaaacacc tgtctctatg tagtgaaaag ctatcaatac ctcatgtacc
     6601 taaacccgtt ggtgataatg ggctctaccc caaaatagcg atataacgtg gtcaaagacc
     6661 cgctaagtgc ttccatcaca aaatgaagtt ttgttgctgg caaacaagtt gttctattta
     6721 atctgattac atttactaaa attaagagtt cattttgctg tactttcaat tttcagtagc
     6781 atgttttcac aaatatcctt atggaaactt gaataaaacg ttttttaaat ccagttttca
     6841 ttcgcagtaa aggcaatggt tagccaacgt tgtggcgtat cttctccaat agccaacgcg
     6901 atttcattac gaattttatc tagagtttca atgcttgaaa ttgggtaatc cataggtact
     6961 actatatgta tttcaataaa ttgtgcgcgt cctatttttg caacgtagct tgtataagtc
     7021 ttgaaatcat gctgtttaat aagctcatct aaaaactctc ttattctttc atcaagaccc
     7081 acaggagcca tgcgaaatat gtctcgcatg gcatcacgga ctgcggacat aggcacaaaa
     7141 atcagaaatg cagtaagaat tgctaaaata agggggtcaa tgtaaggggt aaaatatcca
     7201 taggccccac catgcaacaa ggctgcgatg ccaaatgcca ataggagaga agtggaaatc
     7261 aacgcagaca ttaaccaact ttgaatgtct agtcgcaaga attcggactt aattttgcgg
     7321 tttgttttaa ctaaataaaa atacatgcct gttgataaaa aaaacactaa aagtgcaaac
     7381 acaaatgccc aatcaaaatt gagttcatgt ccaccagaca tcaaactgcc aatcgcgttt
     7441 accagtgcat aagtataaag aagtattagg atactgccat tcaaaacaag aaccataggt
     7501 tcaatatgcc agtaaccata ttgaaatctc cgattcccct tacttgttaa aagacgggca
     7561 acaaaaaaag cagggatcga tataacggta tccaccatat taaacatgcc gtcaaagacg
     7621 atagctaatg aaccggacaa tagcccaaat aaaataccta ccacggctaa gagaaaggta
     7681 acagcgattg atattttaag agctcttcgt tcatgaagtc ccttttgtat tgtcatttga
     7741 gtgatatcct gattttaaca atgctttatc tacttatact attgcttcaa cagccactaa
     7801 taaatccata ggcttgtatt attaggcctt ttacataata actgcttcat ttttcaattg
     7861 ttgcagtaag tttttcgcat ggtttagaaa aatcaattta ttttacgtac attaactttg
     7921 cgtcctttta attttccact ttgtaaatat tggtaggctt tatctgcttg gctgcggtga
     7981 attgcaacat aggagtacat agctgtgatg ttaattttgc caattgtgtt gcctgctaat
     8041 cctgcatctt tggttaatgc cccaaggatg tcgccagggc gaattttatc ttttttaccg
     8101 gaagcaatac agagtgtaac catttctggc actaagcgag cagtgttgtg attttctaat
     8161 tcattaatgt ttccccaatg aatggggtgc gggtagttat cttcaatggc gcaaattcgt
     8221 tgtgcatctg caggcgttac aatgcttaag gcaatgcctt tactcccagc tcgtcctgtt
     8281 cgaccaatac gatgaatatg gacgtcgtga tcaaaagcga gatcaaaatt aatcactgcg
     8341 gaaagttctt ttatatcaag tcctcgagct gcaacatcag tggcgacaag aatagaacaa
     8401 ctttggttgg caaaacgcaa gacagcaaga tcacgctcga tttgttccat atcaccattt
     8461 aaagcgatgg cactgaagcc ttcattgatg agctggtctg tgacttctat tgtttgttgc
     8521 ttggtattac agaagattaa cactgaaacg ggttgatagt gcaacagtaa tgattttaat
     8581 aagggaaatt tctgggtttg tttcgttact tcataaaaat gttgttcaat atcaatttca
     8641 tcaagaggag tttcaacgta aacttctttc ggattcttca tgaattgttt tgaaagttgc
     8701 ttaatttctt caggataggt cgcagaaaaa agcagggttt gtcgttgttt gggacaaacc
     8761 gaaataatgt ttttaatgtc atcaaaaaaa cccatatcca gcattctgtc tgcctcatcc
     8821 aaaactaaag cttttacctg agataagtct aaagaggcgt tttttaaatg tttaagtatt
     8881 ctccccggtg ttcctacaat aatatgagct ccatgccgta atgaatcaag ctgaggctgc
     8941 attggtatac caccagataa attaataatt ttgacattag gcataaaaca ggctaatcga
     9001 cggatagcct ggcttacttg ttccgcgagt tcacgggtag ggcaaagaac caaggcttgt
     9061 actgcaaaaa aagaaatttt taaattattt aataaagaca aagcaaaagc agcggtcttt
     9121 ccactccctg tttttgcttg agctatcaga tcttcatttc taagaattat gggcaggctt
     9181 tgcatttgga taggggtcat attttcataa tttgaagaag caagactttt tattaattct
     9241 tgacgaaggg ggaattgaga gaatgatagg tgttggtcgg tgtgttctga ttgaatcatg
     9301 gaaggtgctt attgttataa ataggtgaat agcttagcac tttttagtgc aattttaaat
     9361 caatttattt tgggcaagct taatctgatg cttgcaattg tcttttctac ttgaatccgc
     9421 gtagatcaaa ataacattaa aatcatctca aagacatcaa cagagagtga ttttaatgtt
     9481 tctatcagat gtatcattct acaaagagat ataagtaaaa atgatattag ttacttaaat
     9541 taaaggggtg ttatattttg tgcttgaaga ccttttgctc cttgcgtcac aatgaattgt
     9601 actcgctgac cttcagtgag ggttttaaag ccagaacttg aaatttcttt aaagtgagca
     9661 aatacatccg ggccgccttc ttgctcaata aaaccaaatc ctttgagctc gttaaaccat
     9721 ttaacgactc catttactgt tttagacata agtatcctta agtatttatt ataatgcctg
     9781 tataggcgaa tttagccgga aaccggtcaa gaattaaaaa ctaccgaacg agggattaaa
     9841 tgaataaaac gacgatttgg aaagtataaa aatagacatt ttctagctga gatcagaata
     9901 ccattcttta aggctataag tcaatcttgt ccttattgag gcataggtaa caaaatattc
     9961 cggagacata ggttacactc aaaggtaaaa tggcgcgctc ggagggactc gaacccccga
    10021 caccttggtt cgaagcctga tgctctagtt ttaaattcgt tataaatcaa taccttgtga
    10081 agcgtcaaat tcaccaggaa ctgcctaaaa ctgcctatta cagcgtagtg ccgccacagt
    10141 tgagacacgt tttggtcaca gaaaaaaatt agtcattttg tcataaatta acttcaatgt
    10201 gttatttttt tattagggta aaaggatttt cttaatgcaa aacttgaaca acacaatttc
    10261 tggtttccaa attggaaatt atagtacaac cccatttcta tggagcaaga attgataagc
    10321 tccttgagga ctatggtcaa aggtcaatat tgacaaaaaa cagcttgggt agtttaatat
    10381 taataatata gttaaactat aagtatattt atgtcatcaa aaataaactg gcttctgaat
    10441 aatattgcct caggtgaaat cttgctccag tcttggttat caagccatgg aattactcca
    10501 caattagcaa gaaaatattg tcaaagccaa tggttggtaa aattgggggt aggggtttat
    10561 tatcgtgcag ggcgtaaacc tagatggcaa aatgcagttt attgtcttca gaatcagagt
    10621 cggtacatga ttcatcttgc tggtttaacc agtttggctc agcagggcaa atcgcattac
    10681 ctacagtttg caccagactc tatttggata aacgttcaag caaaaacgat tttaccaaag
    10741 tggtttaaaa attttccaga attagttatt tctgatgaac taataccaaa ttgggaaatc
    10801 attaaaacat ccaagttaaa tgctcaggat gagaatgact taactactat agaagtagat
    10861 gggattaagt taaaagcaag taatcaagag ttagccgcat tcgaggtgct ggaggctgta
    10921 ccttccactt tgtcatttga gcatgcagca gagctttttc aaggattggt aaacttaagt
    10981 ccacgaaaag tagaacatat tttaaaaaga agcggatcta ttcaaacgaa ccgacttttt
    11041 ttatttttgg gtcatttcta taaacatcca tgggctgaac gggtgaatga gtccttaatt
    11101 aatcttggtg caggtaagcg tcaaatagtc cctgatggaa aattagaaaa acaatatcaa
    11161 atcaccgttc cagaacaatt tataagtagc gattaaaata tgaataaaca gtcaccatat
    11221 tataaacagg ttttgttgct cattcgttta attccactcg tcgaaaagga aacttgtttt
    11281 gctttaaagg gaggtactgc cattaattta tttgtgcgag atttcccgag gttatctgtg
    11341 gatatagatc tagcgtattt acccctcgag ccacgtgaga ttgctttagc caatgtgcgt
    11401 aatgcattag cgggtattgc ggcaaatatt aataaaattc cagatttgaa tgcagttttg
    11461 caagataaca aaccagatga aatgcgaatt attgttgaag atcattcttc tctaaatagg
    11521 gggacccaaa tcaagataga agtttcacct gttgcgcgtg gaactctttt gccgccagtt
    11581 gagcttgatg ttgttgcttc ggtggaaaat gagtttggtt ttgcgtcaat gaaagtcgtt
    11641 tctttgcctg atctctatgg tggtaaatta tgcgctgctt tagatcgcca gcatcctaga
    11701 gatctttttg atgttaaggt tttgttagaa aatcaaaaga ttgaccgaga gctttttaat
    11761 gggtttctta cttatctttt aagtcacaaa aggcctattt cagaaataat gaatccacga
    11821 tggaaagata taactcaagt ttttgaacgt gaatttaaag gaatgactgc tgaaccagtt
    11881 accctggaag agctaacagc aataccagga aaaatgattc agggtcttaa agataatttt
    11941 actcagcagg attatgattt tttagtctct tttaaaaggg gcgagcctga gtggtctctt
    12001 gcatgtagcg atcagattca atatctccca gcagtacaat ggaagctttt aaatattcgc
    12061 aaaatgccca aacaaaaact catggattca gttagcttgc tacaaagaac gatgggacat
    12121 tggcttaatt agtattattt ggtttatatt tttatgtaca attaaactat aggtgtaagt
    12181 atattaataa atgtaaacta attacttttt taaagttatt atgtgtaggg tgcgggactt
    12241 tcgatgagac ttcataatga gtatttaaat ttgaagcata tgttgccatg cttcaatgat
    12301 cataaccatg gccaatgtat ctaattcgca atatctcaat aaggcatttc taagttcttc
    12361 tcgctcagaa tcactcatat ttgtaaactg taattttgca taagctattg ttgcggcacc
    12421 accttctttt aagtcttcgc tatcgaataa aagtggagca tcttcatcag agatatcttt
    12481 attaattggt ggtagcaagt ggtagggatc ttttattcga ttattttctt tgactatcca
    12541 gcattgattt ataaagttat ggctttgaat tccacttaca gtaccataaa ttggttggtt
    12601 atatttttct tgaaggaact ctgagcgatt taaaatcgca gggaatactt gtttaattga
    12661 attagaacca ttagttagag gatcataata aaaacgttta actaattcca aaatatcaac
    12721 catacaacgc ggtccttccc attttacctt gctatcagat ggtgactttg tgatggagcg
    12781 tataaaggag cataaatctt ctttatcatt aggaggttca atcatttgtt ctagtcgttc
    12841 aagaatagaa ttaagatagg tgttttcatg gttactatat ctaaatattg ttccatcatc
    12901 attttctaat tcctttttta gataacgcac aaattcgatg cttggatcaa ctccaggctc
    12961 agaattaaga tactggccaa catgggcaac ttgaccgttt tcattgagtg tatgatgtga
    13021 gaattgaaag gcgatacctt gataaggatg agaaccctta ttaaaaggaa ttggcagcat
    13081 agctgtttca aagtctataa agtgaagggg atatcgccaa gaatcaattt cagtctttag
    13141 gttttcttta tcaatccaga cactattgtc ttgagtttta attttttcta tctgcagcca
    13201 ttgcctttct gttctagaaa tacctgactt atccgattcg tttattttga aagccgtttc
    13261 gttaatatct tttagcttaa ttagcccttg ctgaaaatat ttcggtttat ctcggcaatc
    13321 ccataaatcg agaattgtag ggtcattaaa atctgcttct ttgtacccta gtgattcacg
    13381 ccaacattct ttaaaaccag ataggaattt gtctgattca tttgtattaa attggcaatc
    13441 accacattcg ctttttgctt tagggggaaa cctctgccca ctaaaatata ttttgctgaa
    13501 attgtgaaac tggtctttta ccgtttgttc ttcgtcattt ttttcttgcc aaaataattc
    13561 gatatgatgg tcaacattta tttctcttag gatttttatc gataaatctt cttctgttat
    13621 tggtgatgtt aatgtgacaa tagccttacc tttgctatct ttttgaacca gaaacttttg
    13681 atttaagcca tcagttggac agatagcatc tttatcgaca agcattaaat aactacttat
    13741 ggtgaaggag gggtagagtt gctccagaac ccatttttga aaagcgatat cagcaatata
    13801 tggctgccat tttttagttg ccatcttaga ttcatctttt ttactaattg attttgcttt
    13861 aacttcaatt attttgatat gccctggtct tttttgcagg atatcagcac gcagaaaata
    13921 gttttgatat tgaaaggagg cctcaaatac agttattgaa tccctttgaa ttaactcttg
    13981 agttttgttg agtgcctccg ttgtacttga gctcttaatt gatatgccat ctggaaaata
    14041 acatctagcc aatgcttcta cttgagcgcc gccttcagcc aatgctttca gaaaagagtc
    14101 ttcatccagt atatttgtat attcatcagg tcgatcataa taccagagct tagttggaca
    14161 ttctaaagcc attttgaact ttgatttagt aagtagcgtt gccatccctg tctctactag
    14221 tattggttta aatattcatg tcattaagtt tagttgataa aaataagaag ccccaatctg
    14281 gggcaataga tagatttatg aattcttttg cgatgaaacc tatttacaaa atagcaagag
    14341 aactatacaa gaggcagcaa acaatgtggc tgaagatacc aatgcaaatc gactgaattt
    14401 ctgtgtcttt tgtgtaaagg agtgggcctc aatcaatgaa tccgagatca gtttcttcat
    14461 agcttgaacc gattctttag tagattcgtg cagcaatctg gttatagctt ctttactact
    14521 agctaatgcc gcattgagaa ccttttcagc tttttctttg gcatcatctt tccattgtga
    14581 ggaaatgcct tccatttcct ctcggaactg taccagcaaa tcctgttggg ctttccgatt
    14641 atcctcaatc agttgtgcat tcatagtctg aagcatgagg attggatcgt ctttacccaa
    14701 aagcacacca tgtttgacag tgatgtcttg aatgatttta tccagttttt ctgacattag
    14761 atcaccctgg cgccatccag ctgtccgaac agttgatcgc gaacgatttt taagcgttgg
    14821 cgggtcataa tgcttttttc tggcgtaccc aaaacttcat cgaaagtcaa tttctgttga
    14881 agcatctcgg ttagatcccg gccataggtt tcttttttca gatcggggat atgaattaag
    14941 gctgatattc tgtctttgtt atctcgatag gcttttagtt gttcaaatgc tttgccttca
    15001 tgttcaatgg ctccccaata gggattgagc cagacaacaa actgtgcttc atttgcaaac
    15061 tggtcaatca gctgtgcaaa accattaata gtatcgaata aggcctggcc accagtgatg
    15121 accgtatgaa tcaccatttc atgacctaat tcctgtaaca atgcgggaac ttggttgcta
    15181 atgaggtaat gagatagcgg gacaaaagaa ctggctccgt tgtcaatgac tacatcatta
    15241 gttgttgagg caattttttc gatgagagca tcaaaaagac gggggttaat ttcatcaccg
    15301 ctcatgactt gaatatgatc gacgttgagg gctttaaagc catggaaggt tgaattgata
    15361 gggtcggtgt caatgcacag cgggggctgg cctttgtgct gtttgtattg tgcagttgtt
    15421 gctgaaataa atgatttgcc gacaccgcct tttccctgca gtgtgatatg tatttttgcc
    15481 attacaagag ttcctccaag ttgggtttgg gattaaaagt aaagccccga atttcggttt
    15541 ttgtcttact tttagcctgt tcatcttcct tggtgttttc ctgcatagat ggcttttttt
    15601 ctgcagcaat caaacgtgct acatagccac aaaaagtttt atatgaaaat gagattttgc
    15661 cttcctcaac taaggtttcc cagacaagct ttatagacca gccgtcagct agtgctgaag
    15721 cgatatcatt ttttaaagca agaaatgcgg ctttatttac agatttagca gaggcttttc
    15781 ttaccatttg ctttgctgcg attcgttcgc taagggagtt ttccatctca taccatcaac
    15841 ttattattgt tctctttctg atagtacaaa ctattttgtt gctgtgcaat tagttaagtt
    15901 attttctttc cttttagcgt aattaatcat catcttaaac acacaaatat gtaatttatc
    15961 gtaataattg gtaattatta gaaattaatt tggttttaaa acaaatagcc ttgaaattac
    16021 ataaaattac ctgatagtat gctattagat gaacttaaaa cgcagggcaa gataggtgaa
    16081 gttagctaac cagcaagctg gctaccaaac ctcacaagtc ttattcgcac cttcggtgct
    16141 caacgaaaaa ccagtgtttt gagatcaact taatacccaa aggattgcat aaccacttaa
    16201 ttaagaggtc ggcatcctta tggacgataa gcttaaaaca cccaccagaa aaaatgggcg
    16261 tcatcttcgc gttcctgttt tgccttcaga agaaattcag attaaatcaa acgcagcaac
    16321 agctgggctt tcaattgccg agtatttaag aagaattagt ctcggttatc aaattcaaag
    16381 tagtgttgat aaggattatg tactggagct agccaaaatt aatggagact taggacgact
    16441 tgctggactt ctaaaactat ggttgactca agataaacgt gttgcgcatt ttgacctcca
    16501 cacagtcaaa acattattaa atcgtattca agccacccag gatgccatgt tggaagtggt
    16561 gaaaaaacta tgattatccg ccatatccct atgaagaaaa ctagacttag tagtttttcg
    16621 ggtcttgttc aatacctgtg taatcaacaa aacaaacagg agcgagtggg taaagtcaga
    16681 ttatcaaact gtaatagcct cgatcctata tgggctgttc aggaggtatt agcgacacaa
    16741 gctaaaaacc aaagagccac cggggataaa acctaccata tgcttatttc tttcgctcca
    16801 ggagaaaatc cttcccctca agttttacgg gaaattgaag atagagtcgc attgtcgatt
    16861 ggatttaaag aacaccaaag aatctcagtt gttcatcatg acacagacaa tcttcatatt
    16921 catgttgcga ttaataaaat tcatcctcaa gcctttaata tgattgaacc ttacagggca
    16981 tataagatct ttgcgcaagc tgcatcccag ctcgaaattg aattaggtct tcaaataact
    17041 aatcatcaaa ctcggaaaaa ccgttcggaa aatcttgcag atgatatgga gcaccattca
    17101 ggtattgaaa gcttaattaa ctggatgaag cgtcattgta aagaaaaaat agaggcagct
    17161 agaagctgga aggagattca tagtattttg gcagaacatg ggttgatgat acgtgttaaa
    17221 gccaacggtt ttgttttctg caatccgcaa gggatgactg ttaaagctag ctctatttcc
    17281 agaacctttt ctaaaaaaaa tctcgaatcc aagctaggtc ctttcgtacc atcctactca
    17341 gagggcgatg cacctgagtc taatatttac cgttatgaac cattaaataa aaagatactt
    17401 ggctctgcgc tctatgccac atataagcat gaaaaatcac acaataaaca cattatttca
    17461 gacaaactta aaaatttgcg ggaggctaga gccaaactaa ttgaaaaggc taaaaagcga
    17521 ggtcgaatta aaagagcgac tttaaaatta atgaaccttt ctaaagtaca gaaaaaattt
    17581 ctatatagtc acatcaacaa aacattactt aatgagattg atagcataag aaaaaattac
    17641 gccaaagaac gaagcaggtt attagattcc caccagaaca aaacctgggc tgattggttg
    17701 aaacaaaaag cacaagaggg cgatcaggag gccttagtgg caatgcgtta tcgtaatcgt
    17761 aaaaataatt atgattacac catttcaggt aaagacttag tttctactat tttcaatttt
    17821 agacaaatcg actctgttac caaagaaggc actgagattt ataagaaaga tatgagtgtt
    17881 attagggata atggaaagga aattttcatt tctaaaggtg gttctatttc ggctcttaaa
    17941 agagcgttgg agatggcgcg gcagcgttat ggtgactgca tttgtgtgaa cgggtctcca
    18001 ctatttaaaa agattatttt gcaaattgtc attcaatacc aaatgccgat cacttttgct
    18061 gatttggatt tggaaaatca acgtcaaaaa ctaaactttg agcaggagaa atctcatgag
    18121 caatcaagat caagtggaca aaaccatcga ggaagaactc caggaagcta tgagactatt
    18181 ggagcagcca atgaaaatag aaagaagcga ggcacaaagc ccaacacttt cagcattaga
    18241 caaggctcgc cagccaaaga tcacaacggc ttgcgaaact tgtcccaatg cgatctggtt
    18301 cagttcaccg gaggaggtca agtgctattg ccggataatg cacatgatta cctggagcgg
    18361 aaaagattca aacttaataa ccatgtgcga cggaaaattt ttggaataaa tgagaaaagg
    18421 aaaaatcgta aaaataaata aattggggtt aatttatcat ctatcctata gttattagat
    18481 ggggtaatta agaaaaacca cactgacatt aggaataacc agtattctaa gtaatttaag
    18541 gaaagctatg gatgataata aattaatttt ggatgcggct aatagctatg gttttaattt
    18601 ggaattccga gcaaaaaaga tactggaaga aaagagtttt tctgttttaa tgaatcagtt
    18661 aataaagaat ggggatgaat ttgtagagat cgatattaaa gcagcgcaat atcaaggtag
    18721 ggaatggcta atcgaatgta aaggggctgc caattcatca tatctaatct tgattaaaga
    18781 agataatagc aatgatccca aattgtataa tacaaggcga catgttatta aagattctaa
    18841 ctatcgggtt gcccaattca aaccagacga aaatcagtgt tttttcacgt ttaccggtga
    18901 tttttttaat aaaacaggta atcaactaaa aaaagcatct aagaatgact ttgaaaataa
    18961 tttttttaag gctcaaaatc aaattttgtc agctataaag gccatatcaa taacagatgt
    19021 agataaaaat aaatcaagag acttccccat cataatcccc gttattatta caaatgcaaa
    19081 gttatgggtt attgattaca ataattctgc ggccccagaa gttagccaat ataaatgggc
    19141 tttacataaa ataccaacta aaaataattt atgtattgct cccaaaaatg aaagaaaatt
    19201 tgactcaatt tcaatactgg tattaaatat tgaacatctg gaagaattcc tgagcactta
    19261 ttcattttgt gaagacggag aaatatcagt tggtaatggt ttcatttgta atagataaag
    19321 gttcaaacct gacagcctga cctttaccta tcatgttgaa actaggaaat atgaatcacg
    19381 ttactattct ttgaccatcg catacgatta tagcgtttag caatttgttc aactgttggc
    19441 tttatctgac gtatcactcc ttcgaaaccc aatttgctat gatttgccaa gtcattaaaa
    19501 tcactgaatt gtttagggtt tgatgactgc tcacttgatg cgaaggttgg tagaacaata
    19561 aaaccattaa cagcctcggc agcttcgctg gctttttctt taccggggtt gatacccttc
    19621 gataattcca aatacttgtc atcatccgct gccaggatga ttggtttatg tggatatttt
    19681 ttattcaatt ctttagctac tgctttaagg ttccctgaat caaaggcaga aacgacagcc
    19741 ggtagctcac atgcttcttt tattgttgca gcagtagcat aaccttcggc aattataatt
    19801 acaggtgcat tagcaagctt ttccaaacta cccaacacat gaaaacagcc ttcctttttt
    19861 gagtcttttg caaatctttt agtaccatcc tcagaaatat attggacagt ccaaatagtg
    19921 ccttcgcaat ctgttgctgg aatacaggtt agtttttttt cttcatcggt ataaactcca
    19981 gaatgaacct gaataccttt cgatttcata taaggtgtgg gttcaacaac ctctgtcatc
    20041 atagataatt tttgttggag tcttaacgca gtactttttt gtttgacttc aagctcacgt
    20101 tggcgagttt gttgatgttc aagagctcct gctttaagcg ctgccttttg ttcaccagtt
    20161 aaaatgtagc ccttacattt ccagttaagc tccgcaccag ttcggttgtt tttaatatat
    20221 cctgctggaa taccatctaa atgagctaca taaaatcctg ctttttcacc gtttttgtcg
    20281 ccctccgttg gaatgcgatg aggtttacca tccataattg gatgcccacc tgcaacaata
    20341 gcaccaaggc ttatcaatgc cgctttaaat tcatcctcgg gtataagcgc tttttcctga
    20401 gttaatacct gatgttcagg aagccaggct ttgattttct caacaggaac accagggttt
    20461 gcataccaac acttttgcac tttatcccaa acgatacctg gcgttccatc agggagtttt
    20521 ccaattgtgg atttggcaat ctctttttgt ttgaagggga tgcttaacca gactttttca
    20581 gaagttatgt tgtcagttgc catatagact tcctctttta gtttatcatc cttgatctca
    20641 ataagttctg acagttgaag gttttgggtt tgctctaaag cacacagatg attcactatt
    20701 ttctctgcat ctgcggatgc tctgaatatt tctaagggct catcttccaa aacccgtatc
    20761 catgatttga tatatgctgt gtgttgagaa gggtcatggc ctatacccag ttcggcacct
    20821 aacagcatac ttgcgatttc tgctcttaat tcttctttgg cataggcatc tgaaccaaag
    20881 ggatggccta aatctctatc caaccttgag ggatgaccgc tccaatgacc tagttcatgt
    20941 agggcagtag catagtagtt ggccgcagat ttaaattgct ctttaggggg aagatgaatg
    21001 ctatcagtcg ataatctgta aaaagctctg tctgcttctg agtgaaaaat gttagcgcca
    21061 gagttatgta gcagtttttc agctctttca attaaggacc aatctggctc ttttgcaatg
    21121 agttctggca tattatcaat ttgcgaggca tgaaataccg ttgcataaaa cactttgggt
    21181 cgttcaagat tcacctgcac ttttaatgga ttaccctgtt catcaagaac aggtttacca
    21241 gtatcatcat gtttggtttg ttcctcatga aatttccaat attggattgt ggtgcctttt
    21301 tctcccttgc gaacttgtgc atccatactt tgtgcttgtt tgtaggttaa ccaacgattg
    21361 tcattacttt gattcaccat caaatacaat gcattaattc ctcgataacg cttcccggtg
    21421 actggattat atggaatttg accattgcca acacctggct cccatggttt taaccaggga
    21481 gcggttcctg cttttaggct ttcaataatt tgattagcaa cgacttcatg gtaaggcatt
    21541 ttagtcataa atagcctcca tcgcatcttt ttcattgagt gcatcttcta caaatgctcc
    21601 catgctgtcc gcttctatcg gactgacttc aagaagaaat ccagggatta ctaaatcctc
    21661 aaatttttct gcaatcacat ccattaattc ggtttcatga ttcatgtggt tctctcccgt
    21721 ttattaatta cccatatggg tgtgatcatt cctttgattt gtcctgcgtg aacaagacca
    21781 taatatcgac catcaaatga ccattcgctt tgactggtca tagtcatcac ttcatctttt
    21841 tggagttggt aattcattac ttgccattga ggtaaggcgc gcttcatccc atcctgtatt
    21901 tttggttttg aatagggggt tagtatgtga ttaacaaata cccctttatt ggtcacagag
    21961 aggatatcgc cagaaacagc caccactttt ttcattaagt aaccatatcc atcacagtaa
    22021 agaccatggt ctatataacc tctatccctc gctaacctaa acgattccct gtcatcagga
    22081 caaaaaatca cataagaatt tttgattggg gctttgctgt cgatgcgata taagcctata
    22141 ggaatggatt cagttagatt tatcctaaaa cccatgccat gaaatagtgc gcctacaaca
    22201 ataaggctta tcaagaacat agagatgatg cctgacagtt ttttcataac ctgatgacct
    22261 catcgtcact taaacgagta cgtaaaatat ctgagtgaac aggagcctca actgatgctc
    22321 ttgccatgaa gatcggatct ttaaagtaaa ggggttgttt accataaata gcagggaagc
    22381 ctgctgcata tacgaccata tccccagctt caactatcaa accattagca tcttttttag
    22441 gtcctggcat acgcagacac tcatcaacgg ttaagagtgg tctagatatt tcctgaacgg
    22501 tttttgatat ttgatttaag aacgtagaaa agcgttttcc actggtggtg atgtgttctt
    22561 taacaatagt tgtttgacct gtcagcttgg aaagatgttc tgcggtttca actctgttgg
    22621 gtggataagc attttggata tgacaatttg aagtgatggt ttcatcgggg ccataacctc
    22681 gctcacggct tttaagctga ttaatgtcct gacaaattaa gtaaaacttt aaaccatagc
    22741 cggcaacaaa agccaatgac tcttgcagta tgtcgagttt tccaagactt ggaaattcat
    22801 caatcatgca aagcaatcga tgcttatacg tttttttagc acttacataa gataatcctc
    22861 ggtcatcgga tacacgctca aattccattc tgtcggccaa tagtctcact atcatattaa
    22921 tcatgactct aaccagaggc tgaagccttg ccttgtcatt gggttgagta acgatataga
    22981 ggctcacagg acattcatgg ttcattaaat cgcggatgca aaaatctgag gcagagacgt
    23041 tatgcgcaac cactggatca cgatatagtg caagataaga ttttaaagta gataatacag
    23101 aaccagcttc ttcttcgggc ctatcaatca tgtcacgagc agacgctgat ataacaggat
    23161 gggttttacc ctcaacatgc gggtattggg tcatttcaat gagcaggtca gcaatattaa
    23221 tattgggatc aaccagcatg cggtcaatgt tgggaaatgt ggctggttct ccttgggttt
    23281 tcaatttata aatcgcatgc aggataaaac caactagaag ggcttgagag gtcttttgcc
    23341 agtgggtttc caatccctta ccatcgggat caactacaag cgttgccaaa ttttgtacgt
    23401 cacctacctc tgattctgta cccactctga tttcatccag agggttccag cgtgcgcttc
    23461 cttttaacgt tgcaggttca aatcgaataa ccttgttggt agcatgtttt tgtcgccatc
    23521 cagcggtcat tgcccaaagc tcgcctttta aatcggtaat cacacaggag tgtttccacg
    23581 atagaagggt tggaatcaca aggccaacac ctttccctga gcgcgttggt gcatacgtta
    23641 aaacatgctc aggcccatta tggcgcagat aatgaattgc tcctttgtca ttttcaaacg
    23701 caccaacata aactccttcc tcatgtccaa taaggcttgc actgtttaag tcttcccaat
    23761 tagcccacct ggctgaacca tggaggtaat cactcacatt gttcttttta aaatacttgg
    23821 aagccaacac gatgatcatt aaaaagagac aacccatcat aacgatgaga ccaaaggcag
    23881 cattaaaaga atctgggtaa tagggttgat acttaatagc ccagataaaa atctgccaag
    23941 gcatataaat aggtgcgaat gtccctccaa gggcatcaga ataatgaaat tgatgcgcga
    24001 aaaactgagt tccaatgtgc atgctgatta agaaagaaca aatcactaac aagattaacg
    24061 ttctatttat cttgccgtga tttttttctc tgacttgagg acctatggct ttattaggtt
    24121 tgttttggct cattggctct ctccagatgc cttttggttc acaaattgac taatctcatc
    24181 ggatgcctca aatcctgaac taagactacc tgagggtgtg cccatggata aaatattttt
    24241 tgcgttagat tcttgcgatt cgcaaacgga tgtatctacc ttgttctgaa ttgcttcggc
    24301 aatttttcca cccgttgtgt cagcgatttt ttcagcaact gcttctttaa tcgagttcgc
    24361 tttctcacga gctacatcca tagtgcctga ggctaaatgt gaaccaaaag agcctgcaaa
    24421 tttcgcagca cttcccatcg cttcagctaa gccaccggtt ttagagcttt tatccatccc
    24481 agacagtgca ttggtgtcac ctgagttggt tgattgactg gctgctttaa acgcagcatt
    24541 aagtgcagat aatccaccac cgctttgagc aagtacgcta cctgcggcac ccgccatggc
    24601 tacacctgca attcctgcgg ctcccattgc accaccaaga cctagtccac cgccaccacc
    24661 agaaccgcct cctgacacaa taccagctaa agcaggtgga attttatcta taagaaccag
    24721 caacagcacg gcaacaacta gcatgacaaa catttctttg agcatgatgt tttcagacat
    24781 cgctgcataa tattgatcta caaaggattt accgatacca ataatcaaaa tcatggcaaa
    24841 agcttgtaat gcaataccca atacggtttt gtagtagtta atcgcaatat cctgtgtcca
    24901 acggccaccg ccaaacccta ataaaacaat tccgccataa gttaagatcc aagcagtgat
    24961 taagataatg agcatgttaa tactgactaa agccagaata atcagaatta cccctgcaac
    25021 gagcaatcct actgtagttg ctgcggggga ccagatagat gagctgtcta tggcttttga
    25081 gacaatatca aacccaacat ccacaatatc agaaggggag acatgttcat taattccaga
    25141 agccttggct gcaagttgcc gcatggagtc tatgattgct tttgcaatgg caggtccatt
    25201 gtctaaaatc caatagaaaa aaccgacaac tacaaaaaac cgtactgttt cagctaagaa
    25261 ttcttgaatg tctgctttac gcaatgccat taagccgtat gtccacacca tactaattga
    25321 ggccaaggac cagaaaagat acctggcata actcaacatg gttttgctcc atagtgcgga
    25381 tgtgctggca aaacgctgta aaatattatc cagtaaatca cgactatcca tgccatgccc
    25441 tttggcatgg cagcacgcag aaaacccaaa taaaccaaac gcaatgacat aggggaatat
    25501 ttttttattc atagcattac cacttcttac cagagctttt ggagtaacta ccccctcgga
    25561 aacacgcatc accaatagcc tgcagttctt cttgagttcg accttctctt gggctgctac
    25621 aggtaaggga ggccaagtgc tcagcttttt tctgagcttt ggattcagca ctgtcgcaac
    25681 ctgataaaac acaagcaaca accataattg ataaacataa gatcttcatt tacctctcct
    25741 ttttatgatg gattaccact tttttcctga acttttatgg tatgcgcctg atctgaaatg
    25801 ctcatcccca gcagtctgta tcgcctcttt gttggcactc gctgcatctt tgaccgcctg
    25861 agcgttctgc tgcgctaata acacacctcg gatttgaagc aattgatgag attgattgct
    25921 ggctaattgg gaagcggctt gaagagcctg tttttggcct gcagcatttt gtgcttgaga
    25981 ttgcaaagta cgcagctttg cagaatcatt ttttagactc tgttgttgtt tatcaatccc
    26041 tttgagcatg gcatcattgg agcgcttttc agcaacggaa gctgctaatc tgctttgttt
    26101 gattttttta agttcttcag cagaacattg gccgttgcca ttaaaacaag gagtcttttg
    26161 gtagtattcc tggctttgga atcggtcaag gtagccttgg aggctgccag cctcctgctt
    26221 gtaataatca atggtatcag tcgattcaag caaattattg ataaccgagt tggcattatc
    26281 ccattcaaag gaagtcagtg atttggtgtt ttctagcatg ttttgatatt ggtccatctg
    26341 attcttatat tgatcgactt gggttttata ctcacttatc tgagttgcaa tcatttttcc
    26401 attttgaatc cagtttgcaa cgtcaaacac aggtgcccca cttgcgaata ctggcaaaac
    26461 aaagcaataa ataatcatgg atttaaattt cattatgttc tccttcattg ataagcaaat
    26521 tgcttatagg gctgtttaaa cgttaagtcc ttcaccacga tgatattgaa acgataacct
    26581 gggcgaacat tgatagtggg ggacacattc aggttttttg aaaccagttg cgaagtgacc
    26641 tcacccaact gttgccccaa agcctgactc atgacgctgc cagctgtggg ctgggcgtaa
    26701 ccgaattggt tagattgatt cgtattttgg ctataggtaa tgccagcaat aatgcctgac
    26761 attaaaagtg ctgaaccata aattcgtgca taatgatgat ctacctgatc acgaaatccg
    26821 gcatatccag cactatcagc gccaggcata gagccaatat ccagagcctt actatctgga
    26881 aaaaccaagc gttgccatgc cactaataca gagttctgac caaaagggac atcactggaa
    26941 taaagtccca atatcttggt tccttgaggt atcaaaagat attttccggt agcggtgtca
    27001 tatacgtttt gtgatacttg agcgatgatt tgacctggta attctgaaga aataccacta
    27061 atcattaccc ctggaatcac actgccagct cttagttcgt atcgactgtt tggattttgc
    27121 agcgttgaat ttaaatgcca gcgcatttca ttttcctcac cacctaatgt ttgtggcatt
    27181 ggtttggcct gtcttccttg taataatgcg agctgctctt taaatgctaa tgaagggtcg
    27241 atgtttaatg ccatctcatt gctattcatg gacgttttgg tggtgtcgcg attattcaat
    27301 cgtgggttat cgaccattac ggatgtctta gctttgacag cctcttcaaa ttcttgagtt
    27361 ttttcctgac gaatacgctc gatttcagct tcagatacat cttgtgtcag cgtgtcttct
    27421 ttatgtggaa gttgctcagg aatgggcaaa ggggttgttt cactgttagc tatcgtgaga
    27481 ctctggggcg aaattactgc tgatttatgg tttcctacca cgtcattggc aaggctcatg
    27541 gtatttttct tagggttttt aacctttact atttctggtg gctggttttg cgcattagct
    27601 cgtttattgg caaccaatgc gatgagcacc acaaaaagag ttaaaatacc aattgtaatt
    27661 atgagtggca cattattcac tcgttttaca cctgttgttt gaagcttttg gggtgaattg
    27721 tctggtgaca aataatcatt attttgattc atagctctta ctccttatga gtccatgtgc
    27781 ttgcagggat gatattttca tgcctttttg catacacacg gctcaatgtt tgtattccca
    27841 caaaaaggct tactcgatag ctttgaggtt taagttcatc aagcacgtag aaaaaattag
    27901 ctttattccc agggcattga ttttcattga tgccataacc cttttttctc aagttttgaa
    27961 taagacttaa gccaaagcca tctgttactc tttgaccaat acaaaatgta ttttgagcag
    28021 gtggatatac tcgcgttaat tgagtagctg catctgctgc cagataagca tctttgcctt
    28081 gagacatttg agtgaaattg ccataacgca tgctggcaca gcttgataac aagacagcag
    28141 ccaataaaac acttaatttt ttcatcttca gcaccttgta atcgtaattt tttcttgaga
    28201 ggaaccgctg ccgataacta agatggcttt atcaaaaacc ccatctacga tgtaacgaca
    28261 accctgaaga cgataattga ccatgacgga ctccgatttt ttaaatagcc ctagacttct
    28321 taaaaccagc agcgcaggcg cttcactttg agccatggtt cttggcatct caataatcgt
    28381 tttgacacca ttgttataaa ctctaactgg cttaaatcgg gcattgcctt gaattcgata
    28441 attgaagtta agatcgccta gatattcatt ggtttctggt aaggtatcta cttttcttct
    28501 ttcagcctga atgcgttgta gtaactgcca ttttgctttg gcttcatcag gataagtaaa
    28561 cgaaatgtaa ggcataaatt cggttctatc tgactttaat ctaaagtgat atgttcttct
    28621 gtctgtggtg actaccaaag aagaatcaag ccctacatct ttaggtttta ttagcagatg
    28681 gatttgttgc atagctccag atcctgtgag agatggttca acaacaaagc gtggatcacc
    28741 aacgttcatg tcgttaaact gctcacctgg ttgaagggca atatcacaca cttgtaaagg
    28801 ggcacaaact accctgattt gccctgatcc ataaataaaa ctgactgaac catcgcttga
    28861 tgcaaagggt atacttgttt tttctccttg ttgtgtgcgc tctgcaatat ctaaaccttg
    28921 tttttcttga ctcgatagct tggggacagt atctgaaaaa taatgctcag ccagaatcgt
    28981 gttatccaat gcaaaacttg tgagcggtgt taataaacaa aatagctgca ttattctttt
    29041 cattgctgtt ctccatgctt taattcgtat gaccagttaa aatcacgcac ataaatcagg
    29101 tggggatttt ttaaaatgga ctcactggtg gcatcgttga tttcattctc ttgatacaga
    29161 gtaatcatcg ctttcatcat gcgaggcttt tctttaagtg agccatcacg atttctcact
    29221 gtttcaatcc agtcgacttg ccaggtctca tttgactctt gaagtactga acgaatgtct
    29281 atgctcacaa tctcaaaagc tgctctttca aagggattag attgttgttt gtcgctgtag
    29341 aattcttgaa ccttggcaag ggctgcatca ttggtattta aataggaata cacttgaaac
    29401 actgccttct tttgtagttc tacatcaagg cttaccatgc gtatgttttc aataaaatga
    29461 gcagctgcag cgcgataatc atcgatgctt gcctcaccaa cgctatcagc acgagtaacc
    29521 gataaggtat tgccattggc atcttgttga aacaccaaag ggataaattt agactgacta
    29581 cctattacaa taagtccacc aactgccgcc aatgcgatta agaggctact taaaccaacc
    29641 aactgccaaa cttggagtga tttcatcagt cctgcggtat gaacattcca tgctcgtctc
    29701 gcattgagat aaggattatt cgttatactt tttttcatgc tgcttctccg tattgattca
    29761 tgcgaatacc tttttctaaa agccattgag agacccacgc atccccatgc ttaaactcca
    29821 gttgtttcat tctcgctatg gagtcaggat cagtagctcc aacaaacgcc aaggcataag
    29881 gtccaagcgc taattgataa agacgctggc ctttttcact gacatagtag taatcacgct
    29941 tgggttgggc tgctgcgatg atgtcaatct gacgcggatt taagcccata cgctcataaa
    30001 tggtttgtgt ttcaggatct cttgcataca gatttggtaa aaagattttg gtagcggttg
    30061 attcaacaat aatatctagg attccagagt tggctgcatg tgataaatgt tgggttgcca
    30121 taaacaccac acagtttttc ttagccatgg aatcaagcca ttcagcaatc ttgtttttaa
    30181 acacagggtg agccagcatt agccatgctt catcaagcaa gattagggta gggcgaccat
    30241 ctaacgaggt ttcaatacgc ctaaagagat agagcaatac aggtaaagca aatttctcac
    30301 ctaaacacat taaatgctca atttcgaatg tcataaaaga ggacaagcct aaaccatcac
    30361 tttcagcatc tactaaatgg cccatcaatc catcaatggt gtattgcttt aatgtctctc
    30421 taatcggctc atcctgaata gtcatgacaa attcagatac ggttttagaa ccactttggt
    30481 gcatgcttaa aatggcatgg ccgatttcat tgcgctgaac tggtgtggta ttcaaaccat
    30541 tcaaagctaa aatcgtatca atccactcca tagcccaagc tctatcagct ttagtttcta
    30601 agaactgtaa gggtgcaaag gcaagacgag agtctttatc agcaatggtg aaatgttccc
    30661 cacctgtggc cttacatgtt ggatacatgg acatgccttt atcaaaagaa taaatacgtg
    30721 catctttgta tcgacgccag gacaatgcca gtaaacccag gtgagttgat ttaccagaac
    30781 gagtggggcc aaacataatg ccatggccta aatctctcac atgaagattc aaccgaaatg
    30841 gtgcgttacc acttgtgacg caatgcatca acggaggtga atggagtgag aacagtgggc
    30901 atggtgcttt attctcacca gtccaaatgc tggatgttgg taacaagtcc gctaggttca
    30961 tggtatttat cagcggtcgt ctgatgtttt caacaccatg gcctggcaag ctgcccatat
    31021 aggcatccat ggtattaatg gtttctgtac gtgcgctaaa ccctaacgca ttgatattct
    31081 tttcgatgaa tagggctgct ttttcaacgt tcgctctatc ttcgtccatt aacaccacaa
    31141 ccgatgtata gtaaccttgg cctaccattc cggaattggt ttcggcaata gctgcttgcg
    31201 catcattaac catgccgagc gcgtcttcat caacgacacc cgagttggta ttaaacattt
    31261 ggtcaaaaaa gcctctgacc ttttgtttcc attttttacg gaatttagtg aaatgagcta
    31321 cagcctcatg gggatccatg aatatgaagc gagagttcca gcgatattcc acaggcagtt
    31381 gagtcaacag tgttaaaata cctgggtaag actccatggg gaatccttca atagcaacac
    31441 attgaatgaa ctttcggcca attttaggtg taatccctgg agttaattct tgaccgccta
    31501 ttaaagcatc catataaatt ggatttttgg gcagattcac agggtgatta atcccagtga
    31561 cacaatattg aatgtgtctt aaaaagttat cctgagtgac tttgttattt tcattgatga
    31621 ctagttctga gcctaacctt tcaaggtgaa gtgcagcact catacgtgac tcaaagtttc
    31681 tgcacgattg cttgaaggtt tcaatcagtt gatgggtttt cgctttatgg tttaattttt
    31741 cccctgaatc atcaaacatc aattcaacaa aacgtttttg cgccaataca ggtggatacc
    31801 aggttaatgt gataataaaa taaccttcat acaatgcccc tataccttca aagtactgtc
    31861 ttctttcttc atcaacagct tttgatatcg gatccggaaa gtatgaagca ctctgctcac
    31921 tgtaacctgg agcagcacgt cttatggcat cgacatgaat catccagcca ttacccaatg
    31981 aggacattgc ctgattgatt cgaaaagcca tcatttcacg ggcttcatct gtagtgttgg
    32041 ctatgtcttc accttgatac acccaggctg ccataaaaga gccattttta cctacaatca
    32101 caccatcatc gactactgct gcgtaattca gtaaatcaac cagtcccgct gtatgacttc
    32161 gatgtctcct gacatggtgc ttacggcttt tcatttgagc tgcgctaaac aatgtaccta
    32221 acaacaaaag cccagtaata ctgattaaaa aggtaatgaa tccaatcata gataaccccc
    32281 tttattatta cgaaaagggg tagaacgtgc tggataataa gcctgatagc ctctttgtct
    32341 cagataaact gccctcatat aaggatcagc ttttgccata agccttagac cttttaaaga
    32401 taaaaaccaa agggcaaaac caaaaatagc ggcaagccaa tcctgggctg cgaatactaa
    32461 gattgctgag agtaagcctg aaaacataac cagttcgcgg tcaccaccca tgaaaaggct
    32521 tgggcggtta ccacatctgc gtataggaat ggtacggagg ctcatagctg cgactccata
    32581 gcactcgtta aaactgaggg tttagcaatt tcagctccct tgcctgtaat cgctgtcatg
    32641 gtattttgtg ctgcaaccaa gaatgaaagc accaacacaa taaagagaag ggtccgcaaa
    32701 aaaccattca tttcaccacc aaaaatcagc gtggctcctg caccaactaa accaataatc
    32761 gcagcagtaa aggcaaatgg gcctgtgatg gatttttgta ttttggttag ataggagtca
    32821 aaaggtaatc ctccaccagc acttgatgca catgcaggat ctgttataaa caaaagtaat
    32881 aaaacaatcg ctcccatcat ccatatactt cgataattta tattgactgc ttggttcata
    32941 cctattctcc ttaaatcagt ttttcgatgt tgtaatgacc gttttcatag cctttgaccg
    33001 atatgatttc ttcaactcgg cgcccttgcg gagttcttgc gatatgcacc acacaatgaa
    33061 ctgtttcacc aattaaaggt tcaatttcag caggggcgga gggattacgg gtaatcagtg
    33121 atttcaaacg atgaagtcct gcaatacagt tattagcatg taacgttgca gcaccaccct
    33181 catgaccagt attccaggca tcgagtaaat ccaatgcttc actgccccga acttctccaa
    33241 caaggattcg atctggccgc atccttagag tggtttttaa aagctgggtc atattgacat
    33301 caagagtggt gtgatattga acgcaattct cagcggcgca ttgaatttca cccgtatcct
    33361 caataatgaa gacacgctca gctggcgaac tacgaaccat ttcattgata atggcgttca
    33421 ctaaagtagt tttaccagag cctgtaccgc caatgactag aatgtttcga tgtgcaataa
    33481 cggctttttt aatcacagca cattgtgctt ctttcataat tcccgatttt acatagtcat
    33541 ccagtgtaaa aactgaaatg gcttttttac gaatcgcaaa ggttggtttt gagaccactg
    33601 gcggcagctg tcctgcaaaa cgtgaaccat ccagtggcag ctcaccttcc agtatcggat
    33661 taaaacgggt tacttcctta ccatgaaagc ccgcaacagt tttaatgatg gattcagcgc
    33721 gtgcgtcagt gatactacct atgcaacgca tggtttcgcc taggcgttcc tgccaaagtt
    33781 tgccatctgc attgagcatg atttcaacgg ttttaggatc acttagcgcc tgctcaatca
    33841 tgccacccaa gtcacgcctt agcttttcac gggcgcggtc tttaaccgtt atcccttgtt
    33901 cttcttgtgc tttcatacta ataatcctcg attacttatg tttttaagct gacttcaagc
    33961 catgtttgaa tttgctaatt aaacggtcaa taatgctttc cttattggaa ggagagtcgc
    34021 tgaaccattg atctttttgg cgttctcgat aaatacgtct ttgaatttca tcacggtgaa
    34081 cagggattga ttgaggggca tcgaaagcaa ggcgaacttc accgtcacga taacccacta
    34141 cagtgcagta aacttcctca ctgataacaa ctgattcccc aacttttctt gttaaaacca
    34201 acataatgcg tctccttctg ttatttaaat aaataggccc tgcattgcct gatattctct
    34261 tcaaccactt ctttgcgggc tggttgatat ttttctgatg cgacataaaa agcatttcgt
    34321 cttttgatat cacggcaaaa gccaatcatt tgcggcagtg ttggaggcat ttcgcaatga
    34381 tcgcggcaat caataatgac ctgattcaaa atctcatcac taaattgatt taagccctcc
    34441 aaccactcct tcttggcaaa ctccaaaaat ccatcacttt taaactgact gcgccataga
    34501 tgtccgtaga agaccgcaaa acgagtaaac agattctcaa tacttttttt tctactgtcg
    34561 gtagcattgc aggttgacga cgtttgattg ctggtattcg tcttcaccgg tgatttttgc
    34621 atctcctgtt tctgggttcc agaatggatc aatggctctg cttttagtat ttgtgccgca
    34681 cttctcattc cttttctcct gagttaattc gattttgact tcatcttccc agcatttttg
    34741 agacaaccag ttggctggaa atttccatgc tggaacccat tccccaagtg cttcttttgc
    34801 tgtacgggct ttgatttgtt gagcaagggc ttgcaacatg cattgcagca aaggctcatc
    34861 tggattgatt tgtttgaaaa tttcaaaggc acgttcacgt gactttttct cggggtacat
    34921 atgccagaaa tggtcgaagt agcgtaataa ataaatataa ttattttctt ttatttgagg
    34981 tgtgtcggct tttaggattt tatctgtgtc ggcttttctc tctgtatcct cagaaaccct
    35041 tgtatttaca gggaatacag catgcggttt tgtgtcggct tgctgtgtcg ggtttgtgtc
    35101 gggtttattt tggacacaat aactgatatc cgctaaaaga cattttaaaa taagatgttt
    35161 atcgaaagac tgaatagcaa ttagccctga tctttcaagc cctttaatgg ctcgacgaat
    35221 ttgctgacgg ctaggacttc ctgactcttt aatgccttga tgaggttcaa tataaagtac
    35281 ctcagccagc gattggtaac taatccgtcg cttaatccct actatgaaag tattgcgatc
    35341 catgtaaggt cgaatccctc ttaagtaggt aatttgttgg atatatgaaa gcccacagag
    35401 cgcatccaat tccttgtcat tcactgtaaa gtacataaat aatccgttat tccttaacta
    35461 aagatcttag cgcccaatga aacttgtgat aacattttga gaaattaaga
//
LOCUS       DBNCWK010000019        34773 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.019, whole genome shotgun sequence.
ACCESSION   DBNCWK010000019 DBNCWK010000000
VERSION     DBNCWK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 34773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34773
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.019"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1060
                     /locus_tag="AC06IO_000834"
     CDS             <1..1060
                     /locus_tag="AC06IO_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874046.1"
                     /translation="KALMQELKEAIKIKTLAQSHLTKEEQHLIVVYLAEIKEFYKLNS
                     IFDFFGVENGGAQHGRLFFHDSENNSSRELTELFRLNKALLNDLNEKIEQVAIEYSKE
                     NYDYIWNQIIFSYLKLLEHNQHWQSFWEKESQVLDEAKLSKAKHLVCSAICFKLFSVL
                     SANSIEMMSHPSVKVFIKLSNSINFEDIRKEMRNRLRSEILEDIKIATMTKKNSKLTY
                     KSLYVKVNRDAHFCALSLEEKALAVIAGVADACSGNDAHFKLLMESIVKAKKQFPVDD
                     FNAKVQAILEELQAKNKLPQKYEKKFQEICETQDNKRILKKNLSLPQLASVGSTFYHS
                     KKREGSSDENTSSYPHRP"
     gene            complement(1599..3671)
                     /gene="recG"
                     /locus_tag="AC06IO_000835"
     CDS             complement(1599..3671)
                     /gene="recG"
                     /locus_tag="AC06IO_000835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIO9874047.1"
                     /translation="MLSNSCEYLTGVGPTIAAKLAKCGIFTIQDLLFHLPYKYQDRTR
                     ITPIQDLRSNEWCVIAGHVCKTEIKYGKRMMLNCYVEDKTGVVKLRFFHFNKQQIQAL
                     NNSAMIRAFGEVRGFNNQLEMIHPEYQLIDQESDFHVEETLTPIYPSTQGLTQTRLRQ
                     LVKIALEQSEHELHQLEWMSEKQLQENNFYDLGEAIKLLHNPPPDISLSNLEAGEHPA
                     LKRLIFDELLAQQLSMQFARQSRSKLQAPAIFFDNAIHKRFIESLPFSLTNAQQRVFK
                     EISVDLTQSKPMLRLLQGDVGAGKTIIAALAALQAISQGFQVAFMAPTDLLSEQHTNS
                     LGKWLEPIGINVLRLSGKMKTTERKNTLAALQDNSCQLIVGTHALFQEQVEFARLGLV
                     IIDEQHRFGVEQRLLLQQKGQLNQLIPHQLLMTATPIPRTLSMSHFAHLDISVIDELP
                     PGRMPITTAVLNQDKRELIIERLQAAIANGKQAYWVCTLIEESEKLQCMAATDTSKKL
                     QEQLSFARVGLVHGRMKALEKEATMAAFKQGEIDLLVATTVIEVGVDVPNASLMIIEN
                     AERLGLSQLHQLRGRVGRGNNQSHCLLLYQSPLSQQGTERLKIMRSTTDGFLISEKDL
                     ELRGSGEILGTRQTGFRQFKIANLQRDKTLFAILRPIAKQLVLEKTAIARQITQRWLG
                     NFEQFLQS"
     gene            3818..4876
                     /locus_tag="AC06IO_000836"
     CDS             3818..4876
                     /locus_tag="AC06IO_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIO9874048.1"
                     /translation="MNRIITFTAGLILVWITGYLLIAGRGLLIPIVISIFVWHLLNTI
                     NSGVQKIPFIGTHLPSWIRMILSLFVVVCLVMVLINIITNNVSDVIAASPRYQENLLH
                     IFNSLDQRFHVKGIFDFDSLIQGLSIQSMLVNIYGVFTTITSSAVLISLYVVFLFVEQ
                     HYFTQKMDAFFPQAEHRKLVKNIISHITKDTQTYLGIKTLLSLLTATLSWFIMKWVGL
                     DFAEFWALLIFFLNYIPNIGAIVATAFPAALALIQFESWWPFLEVTSGIVAIQFFVGN
                     FLEPRLLGKSLNLSPLVILFALALWGAIWGVLGMFLSVPITVMMMIIFAHFDVTRPIA
                     ILLSQDGYIHKSYESLEN"
     gene            4980..6749
                     /gene="argS"
                     /locus_tag="AC06IO_000837"
     CDS             4980..6749
                     /gene="argS"
                     /locus_tag="AC06IO_000837"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIO9874049.1"
                     /translation="MRSMKAIIEYLLKQALINLQQSGEMPIDLEIEIKVENAKDPAHG
                     DYATNLALVLAKPCRQAPKVLAERLVAVIPADPSVEKIEIAGAGFINFFMRSTARSLI
                     ISEILNKGKEFGQGNLGQSQKVLIEFVSANPTGPLHVGHGRGAAFGATLGNVLKAAGY
                     DVTLEYYVNDAGRQMNILAVSVWLRYLELAGEPIAFPANGYKGQYVYEIAQEMWSEQG
                     NQFVHPWISVVDNLPADEPEGGDKETYIDAIIARAQSLLGKDGFANFHQHALKTVLDD
                     IKDDLQAFGVRFDSWFSEQSLFEDGSIGKGIQALKDRGHTYEREGALWFRATDFGDEK
                     DRVLVRANGQTTYFASDVAYHWNKYDRGFDRVIDIFGADHHGYVTRIKTAVKALGHDE
                     SALDVILVQFAILYRGGDRVQMSTRSGSFVTLRELREEVGNDAARYFYVARKPEQHMD
                     FDLDLAKSESSDNPVYYIQYAHARICSVLRQLKERSLKWDKDMGLKNLDLLEQQHETT
                     LISLIARYPEVIQSAAASCEPHQLAYYLRELANGLHSYYNAIQLLCEQEQLRCARLCL
                     LESVRQVLNNGLAILGVSAPESM"
     gene            6749..7444
                     /locus_tag="AC06IO_000838"
     CDS             6749..7444
                     /locus_tag="AC06IO_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIO9874050.1"
                     /translation="MAKDYGNRRAARRNRGPHQFLVIIVTFLFGYITASFLDVQTISQ
                     WVNTQVLAQHDDEKAPTKVAQEHKQVPPKPKFEFYTLLANERGPASQASSQQNTDNHA
                     ADKTSTPIQKTANTVTAAVNNSVKSTIANPTPQTPVKVAEAKPTTMQQPNKGAYLVQV
                     ASFKARQDAEQMKGMLILKGFDVSVVPVTQAQGNWFRVVVGPYPNKALAQKAQMNLAK
                     TERLNGMVRSVGG"
     gene            complement(7484..7822)
                     /gene="folB"
                     /locus_tag="AC06IO_000839"
     CDS             complement(7484..7822)
                     /gene="folB"
                     /locus_tag="AC06IO_000839"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIO9874051.1"
                     /translation="MDTLNITALSVETKIGVYGWEQRINQRLLIDISIPFDFKDCNDN
                     LSNTLDYADLCQTVTQFVESTSFQLIETVAEQIAQLIKQKYPVTKVSVAVTKPHAVKN
                     AAGIQVVVNR"
     gene            8062..9120
                     /gene="hemE"
                     /locus_tag="AC06IO_000840"
     CDS             8062..9120
                     /gene="hemE"
                     /locus_tag="AC06IO_000840"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIO9874052.1"
                     /translation="MFDLNQSLFLRALRRQPVERTPIWIMRQAGRYLPEYRKVREHAG
                     DFLNLCKNPELACEVTLQPLRRYALDAAILFSDILTIPDAMGLGLYFAEGEGPRFTNP
                     LQDTKAIHTLKIPSIPESLSYVFDAARLIRQEMPKELPLIGFSGSPWTLACYMVEGGS
                     SRDFKRILNLIYTEKEAAHLLLSKLAASVTAYLIEQIKAGVNAVMIFDTWGGVLTPQN
                     YKDFSLAYMHQIVQQLKKEYPDIPVILFTKNGGQWLEWMAETGCDALGVDWTCDLASA
                     RKRVGGKVALQGNLDPAVLLTTKNCIRSEVGSVLASYGYGTGHIFNLGHGITPDVPPE
                     NVAIMIEAVHEISPQYHL"
     gene            complement(9421..10758)
                     /locus_tag="AC06IO_000841"
     CDS             complement(9421..10758)
                     /locus_tag="AC06IO_000841"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HIO9874053.1"
                     /translation="MQEWSPTTWQKYSYLQAASYADEEQLNKVVEQLSLLPPLVTSGE
                     IKHLKNEIAQAGRGNAFILQGGDCAESFNDCRSEVISNKLKIILQMSLILLYGLRKPI
                     IRIGRIAGQYAKPRSSDYETINGVTLPSYRGDIVNSPEFTATAREPNPKLLLQAYSCS
                     AMTLNFIRALLDGGFADLHHPQRWDLGFVEHSPQKNEYQHIVDSIEDALDFLNAIDGI
                     RSSSISKVDFYTSHEALHLHYEQALTRQLKDGKWYNLSTHLPWIGMRTAQTDSAHLEF
                     LRGVQNPIGIKIGPAATPEWLSEVLSIANPQKEEGRVLLYTRLGAKLIDRLLPPLIDT
                     VRKSKIPVTWSCDPMHGNTETTEDGTKTRHFDNILSELKQALEIHRSMGSYLGGVHFE
                     LTGDNVTECIGGARGLAPHDLKTAYHSLVDPRLNYEQSLEMAIQLSHQFRNES"
     gene            11038..11637
                     /gene="grpE"
                     /locus_tag="AC06IO_000842"
     CDS             11038..11637
                     /gene="grpE"
                     /locus_tag="AC06IO_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIO9874054.1"
                     /translation="MSKQNKKDWKKFRDEHKEEHKVENEILEEETDEESQHQEPALGH
                     PSYTALEEQLTLAEQKAHENWEKSVRALAELENVRRRMEREVANAHKYGVEKLISALL
                     PVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEA
                     MSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK"
     gene            11782..13716
                     /gene="dnaK"
                     /locus_tag="AC06IO_000843"
     CDS             11782..13716
                     /gene="dnaK"
                     /locus_tag="AC06IO_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIO9874055.1"
                     /translation="MAKIIGIDLGTTNSCVAVMEGDKPKVIENSEGHRTTPSIVAFTD
                     DNEILVGQSAKRQSVTNPEKTLFAIKRLIGRRFDDPIVQKDIKMVPYKIMKADNGDAW
                     VRVKDQDKAPPQISAEVLRKMKKTAEDYLGEEVKEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGMDKKRGDSVIAVYDLGGGTFDISIIEIAEVDGEHQFEV
                     LATNGDTFLGGEDFDLALIEYLASEFKKDTGIDLHNDPLALQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADASGPKHLNIKLTRAKLESLVEKLVERTIEPCKTALKDAGLTVSQI
                     NEVILVGGQTRMPLVQKTVEEFFGKEPRKDVNPDEAVAVGAAIQAAVLSGEVKDILLL
                     DVTPLSLGIETMGGVMTKLIEKNTTIPTKATQVFSTADDNQTAVTVHVLQGEREQASA
                     NKSLGRFDLRDIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKAQSIVIKASSGLS
                     EEEVAAMVKDAQSHAEEDKKFKEMAELRNQADSLIHSCEKSMKDLADELSEDEKKGIE
                     TAISELKEAVQGTDKARIEDKLKVLTDASAKMAERIYAKKSSEGQAAQGQTQSQESTK
                     PAEEGVVDAEFEEVKEEDKK"
     gene            13905..15044
                     /gene="dnaJ"
                     /locus_tag="AC06IO_000844"
     CDS             13905..15044
                     /gene="dnaJ"
                     /locus_tag="AC06IO_000844"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIO9874056.1"
                     /translation="MEQRDYYELLEVSRNASDAEIKKAYRRLAMKYHPDRNPGDTSAE
                     EKFKEIQKAYNILSDKQKRAAYDQFGHAGVDPSMGGGPGGFGGFGGFGDVFEDIFENI
                     FSGGRGHGRQSRGQRGADLQFNVQLTLEEAAIGKEVEITVPRHGTCTVCEGSGAKKGT
                     SPKTCETCQGMGQVRIQQGFFSIQQTCPTCHGEGKIISDPCASCHGQGRVRESKKINV
                     KIPAGVDNGDRVRLSGEGEAGVHGGGSGDLYVQISLKKHAIFERHENDLHCEVPISFA
                     TAALGGSIEVPTLEGRVTLKIPAETQTGKVFRLRSKGMKSVRGYGQGDLLCKVVVETP
                     VNLSREQKELLNKLQDSLENAKGTHSPKTSSWFAGVKKFFEDMKF"
     gene            15139..16251
                     /gene="carA"
                     /locus_tag="AC06IO_000845"
     CDS             15139..16251
                     /gene="carA"
                     /locus_tag="AC06IO_000845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIO9874057.1"
                     /translation="MINKSAILALADGTVYEGLSVGAIGESVGELVFNTSMTGYQEML
                     TDPSYARQIITLTTAHVGNTGCNMEDMESTKVWAAGLVMRNCTAVPSNFRAEQSFPDW
                     LSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIAVAKAKSFAGLQGADLAL
                     EVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTS
                     AEEVLAMNPNGVFLSNGPGDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGI
                     TKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQG
                     IRHKTKPAFGFQGHPEASPGPHDIDILFNEFVNLMK"
     gene            16460..17608
                     /gene="metK"
                     /locus_tag="AC06IO_000846"
     CDS             16460..17608
                     /gene="metK"
                     /locus_tag="AC06IO_000846"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIO9874058.1"
                     /translation="MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACE
                     VLVKTGMVLVGGEITTKAWVDVEDITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSP
                     DIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
                     WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWL
                     NDKTRYFINPTGRFVIGGPLGDCGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSA
                     AYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTH
                     FDLTPQGIIDQHDLLRPIYRQTATYGHYGRESFPWERLDKVAELSKAL"
     gene            17627..18952
                     /gene="ahcY"
                     /locus_tag="AC06IO_000847"
     CDS             17627..18952
                     /gene="ahcY"
                     /locus_tag="AC06IO_000847"
                     /EC_number="3.13.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIO9874059.1"
                     /translation="MELVEEGVQKINTLQDYKVADISLADWGRKEIAIAETEMPGLMA
                     LREEFSAKKPLQGARIAGCLHMTIQTAVLIETLVALGAEVRWSSCNIFSTQDHAASAI
                     AAKGIPVFAWKGETEEEYWWCVEQTLSGPNGWTPNLLLDDGGDLTQIVHQKHPKLLTA
                     IKGVSEETTTGVARLYEMAKHGQLKIPAINVNNAVTKSKFDNLYGCRESLLDGLKRAT
                     DVMIAGKVALILGYGDVGKGCAQALRGQGATVLVAEIDPICALQAAMEGYRVVTLDDV
                     AEQVDIVVTATGNYHVVTHDHMKRMRNQAILCNIGHFDSEIDIQSLKQYQWENIKPQV
                     DHVIFPDGKRIIILAEGRLVNLGCATGHPSFVMSASFTNQVLAQIELFQNSSQYQNQV
                     YVLPKVLDEKVARLHLGRIGAKLTQLSNEQADYIGVDKNGPYKPDHYRY"
     gene            19076..20083
                     /locus_tag="AC06IO_000848"
     CDS             19076..20083
                     /locus_tag="AC06IO_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIO9874060.1"
                     /translation="MDLVIAANYLSTLEKSLEQFGINHFLEKIGFDLIRVHDPDAYLT
                     QDEFNQVIMEAYKLSNCPELGLVFGQNLSIVNHGFLGYAAMSSPTFGTAIQTVLSYLN
                     TRTNLLSLELRQFNNENSAYIKLLPHTKEMVLSRFITELAVAHLIKMRNFLISCNDPL
                     LKIELNYDEPPYAKYYQTIFQTKIVFNTEATRVWFKAEELNYPIHFADDVSYQLAKKQ
                     LQELEKKMSLKEDMASKIKSILINQNLNHASMDEIASRLCMTSRTLRRHLQRLDVTYQ
                     ELLDEVREQKAKTFLLNNNISMTEISFLLGFQDTSNFSKAFKRWTGITPTEYREKHEV
                     Q"
     gene            20215..21903
                     /locus_tag="AC06IO_000849"
     CDS             20215..21903
                     /locus_tag="AC06IO_000849"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIO9874061.1"
                     /translation="MGLKSGILGLSLLSLACGSFAMTETETIRVIIKYKQQPATIASL
                     KSQAIKSVQIPVKEMKPMANGAYSLIFDTSNSHAIVKAKEEDVTAKVLERLRKNPNVL
                     YAVKDRIGHFKPLPDPVINQELSQLLSHESQWDEFERPAGIRLESRPGLRDGAWAYTT
                     GKSRKPIVVAVLDTGIALNDSLVNNLVKDNEGNVFGWNFAGNNNDLSDETRSYHGTHV
                     AGTIAGYGDVMNGVGEDLKILTVKIPAENGMFYESAVVNSIYWAVGGDVPGVPKNIHP
                     AKVLNMSFGVDLEPGKEIDYCDEALQEALFFARKKGAVVAVAAGNDNVWEHYNAPAVC
                     NGTIKVASTGPEGLRSYFSNYGPSITLAAPGGDKRYGTSGGILSTVNPGGGYNSSGFD
                     FYQGTSMASPHVAGVAGLIFAVSNKTLKPEQVEQILYVTTHEFGQSNDTNKSCVGKKP
                     CGHGILDADNAVQAALNGYDVLFSAPKMIALATHECGENRLKPGKSIINDSTATWIQA
                     KSSCETGVSYQKPRIEKAKDGKIYAYYGSVSYELDQSAYKECRVVGYDGVGCYL"
     gene            complement(22057..22338)
                     /locus_tag="AC06IO_000850"
     CDS             complement(22057..22338)
                     /locus_tag="AC06IO_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874062.1"
                     /translation="MAETIDDITIAFNENGVETTRELDKQILSKGAWTTIMFKYQDWD
                     NAKNDYGPVKYSIRRYQKRNNQYWQKSKFNISSTEQAKKIVDILNNWLE"
     gene            complement(22352..22954)
                     /locus_tag="AC06IO_000851"
     CDS             complement(22352..22954)
                     /locus_tag="AC06IO_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HIO9874063.1"
                     /translation="MKSILLMSLLLLIMPFRLMSAQSLYCPQNHGYINIGMSMDQVIA
                     ACGQPLSKQESNQPILQKIPVQQLIYNNLGNSDGLYSGSLNVPEGTAFYGVWNIPTGS
                     SGVQLEVDILNNKVQDIRVNGGNTNAFSLCGNASIQIGDPATKVYGACGSPNLVNNTY
                     INQVVPTETKPQIWIYKPGPYQPAFSLTFVDGKLQSIGNN"
     gene            complement(23014..23589)
                     /locus_tag="AC06IO_000852"
     CDS             complement(23014..23589)
                     /locus_tag="AC06IO_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIO9874064.1"
                     /translation="MAGLTAVGYFLLSLLFTIVIFSLWLRIAIRFFRISAINPFSQLI
                     HTITDPLVKPLYWIIKKPLQPGQKYDWIAFGVLILVEFFKIISLSLLMYHAIIPFLFI
                     FLYILVDLIIQPCEILFFAVLIRVIMSYVNPKWQHPLGDFLRVITTPILRLGRIIVPD
                     ISGFDFSPFIIMVILKIITLFLSASLPWKLL"
     gene            complement(23934..24722)
                     /gene="proC"
                     /locus_tag="AC06IO_000853"
     CDS             complement(23934..24722)
                     /gene="proC"
                     /locus_tag="AC06IO_000853"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIO9874065.1"
                     /translation="MNISFIGYGNMAKAIACHLQKQNKYSLSAASPSLSAGISKENIR
                     THYDNKEVAKDANVIILAVKPAQMREVVNEINPFISSGCLLISVAAGLSLTWFAKYCK
                     KGQAVVRTMPNTPAIVGFAATSMIANEFVNEKQKKWAEQLFSSIGITDWVKDEEDMDT
                     LTALSGSGPAYVFLFLEAMINAGVQLGLEETVAKHFAIQTFSGSLQLAQHSQLSLTEL
                     RKKVTSPGGTTAAALEILHGDLDELIFSAMKAAKQKAHQLGKVE"
     gene            complement(24719..25414)
                     /locus_tag="AC06IO_000854"
     CDS             complement(24719..25414)
                     /locus_tag="AC06IO_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIO9874066.1"
                     /translation="MSISQNINKVRELITQTESSSNRQPNSVLLLAVSKEQSPELIRE
                     AFQQGIRHFGENYFQEAQEKILALRDLPICWHFIGPIQSNKTKGIAKYFNWVHSIDRN
                     KTAKLLNQFRSEQLPPLNVCLQINLTGETTKSGIPPEHAIDLALEVKQLPNLQLRGLM
                     TIPPQQNNMQTQYNLFLKLNQLKESINKALHLKMDTLSMGMSDDLVPAIKAGSTIVRI
                     GRAIFGERKGKLL"
     gene            25577..26611
                     /locus_tag="AC06IO_000855"
     CDS             25577..26611
                     /locus_tag="AC06IO_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIO9874067.1"
                     /translation="MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEH
                     KDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLARFRVNAFNQSRGAAAVFRTIPSE
                     ILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
                     PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAA
                     ETGHLVFGTLHTNSATKTINRIIDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGR
                     VAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHLTELVNKNIISKHTA
                     HEAALNKSLF"
     gene            complement(26941..27648)
                     /gene="rph"
                     /locus_tag="AC06IO_000856"
     CDS             complement(26941..27648)
                     /gene="rph"
                     /locus_tag="AC06IO_000856"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIO9874068.1"
                     /translation="MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIV
                     EGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACI
                     DLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVA
                     AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSML
                     SLAEIGIPQLIEIQKNA"
     gene            27949..28815
                     /locus_tag="AC06IO_000857"
     CDS             27949..28815
                     /locus_tag="AC06IO_000857"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIO9874069.1"
                     /translation="MIHSMTAFSRIQKQFDVGDFCWEIRSVNHRYLDVSFRLPESFRF
                     LEAELRTLLRDKIHRGKLECHLKYKDTSCDNRSMLINIGTINALLDLSSKLSASHHLA
                     NDMTVSQVLSWPGVVEANLLDMEELSQIALELFQKSIDKLCEVRAGEGRSLKAYVEDR
                     LQALRQEVMKSKSIVVSMSAKTKDKLLTRLHELQMDVPEARVEQEIALLLAKLDVSEE
                     LDRLETHIAEVSRTLNAEKVTGRRLDFLMQELNREANTLSSKSDSAELTLCAVEMKVL
                     IEQMREQIQNFE"
     gene            28821..29450
                     /gene="gmk"
                     /locus_tag="AC06IO_000858"
     CDS             28821..29450
                     /gene="gmk"
                     /locus_tag="AC06IO_000858"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIO9874070.1"
                     /translation="MVSDNSGNLYIVAAPSGGGKTSLVKKLIEMVGEIEVSVSHTTRP
                     MRPGEKEGVDYFFIDEEQFISMVNEGAFIEHAKVFNHWYGTSVAQINKRLQFGIDVVL
                     DIDWQGAEQIRHAYPDAVSVFIIPPSLDALKERLMNRRQDKDHVISERMTKAQDELGH
                     YPEFDYLIVNDDFEKAAMELQSIVIANRLRIEKQVNKQAKLLSFLLSSQ"
     gene            29501..29704
                     /gene="rpoZ"
                     /locus_tag="AC06IO_000859"
     CDS             29501..29704
                     /gene="rpoZ"
                     /locus_tag="AC06IO_000859"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIO9874071.1"
                     /translation="MARVTVEDCLEHVKNRFELVMVATKRARQIAVRGDQPMVEWEND
                     KPTVVALREIAEGYVTADILDED"
     gene            29872..31995
                     /gene="spoT"
                     /locus_tag="AC06IO_000860"
     CDS             29872..31995
                     /gene="spoT"
                     /locus_tag="AC06IO_000860"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HIO9874072.1"
                     /translation="MSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEP
                     YITHPVAAALILARMRLDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTK
                     LTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAI
                     ETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEK
                     DLQHALSQLNIPYEQVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYR
                     ILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADN
                     GVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVF
                     TPKGHIMELPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAP
                     GAHPNPAWLNFVVTGKARSNIRHFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPE
                     TLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANESDKGNKKPLAIKGTE
                     GMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQ
                     GEYWVDITVDVANERGVLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTH
                     LARVMRRLRANKVVMRLYRKKQGEV"
     gene            31995..32381
                     /locus_tag="AC06IO_000861"
     CDS             31995..32381
                     /locus_tag="AC06IO_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIO9874073.1"
                     /translation="MQPINTKLAPKAIGTYSQAIKSGEIVFLSGQIPLDPETMQICSD
                     DIKLQITQVLENLSAVCEEAGGSLANIVKLNVYLTDLSHFPLINEAMSRYFSEPYPAR
                     AAIEVSALPKGAKVEMDGILVLSASA"
     gene            32384..32875
                     /locus_tag="AC06IO_000862"
     CDS             32384..32875
                     /locus_tag="AC06IO_000862"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIO9874074.1"
                     /translation="MSDDQYARTGRLMFLFVWIIFFIGLFLFFYYYDKPESTIFVASR
                     TEYVLSADNEGHYWIKGKINEYPVEFLVDTGATLVAIPQGLAKDLKITGRYPVTIETA
                     NGKVTGFLTRLQHLSFGEFHLQNVKAVIIPENDDNTVLLGMNVLSQFHMIQQGKQLIL
                     KRQ"
     gene            32936..>34773
                     /locus_tag="AC06IO_000863"
     CDS             32936..>34773
                     /locus_tag="AC06IO_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIO9874075.1"
                     /translation="MSIVSLNGASLILAGNCILDRADLQIQPQDRIALVGRNGAGKST
                     LLKILQGELVLDSGQIQRSSGLRISGLVQEVPGAEGESVYHFLVKSLGETGEVLSQFH
                     EFTKQGDMDKLARCQQRMDNLNAWHLLPEIETMASRLGIDIHEQMSNLSGGMKRRVLL
                     GAALLAKPDLLLLDEPTNHLDVEAIEWLENYLKSFSGAVMVVTHDREFLSQIATSILE
                     IDRGKLYLHHCNYEAYLDRRESIRLSEQKQNDLFDKRLAEEEAWIRTGIKARRTRNEG
                     RVRALKAMREQYKARRHQLGTVKSLNLDVSRSGALVVEANKVSYAINDKPIIKDFSLL
                     MTRGDKLGIIGPNGCGKTTLVRLLLGEIKPDAGTIKLGTGLQVAYFDQLRRHLLEDQT
                     VMFNVGEGADYVTINGKQKHVASYLKEFLFSSDRFNQPVSSLSGGERNRLLLAKLFAK
                     PVNLLVMDEPTNDLDIETLELLEAVLADYPGTLILISHDRAFINQVVTSVLVYEENGK
                     FNEFVGGYDDYKKHQLTRNEQQTKSPVSKRSSPEKAKNLSFNEQRELSKLPQQIEAVE
                     KKIEALHSQMASPEFYQQDAKLISEVTQLLAKEEALLNQYFARWEEL"
BASE COUNT        10574 a         6042 c         7556 g        10601 t
ORIGIN      
        1 aaaagcttta atgcaagagt tgaaagaagc gataaaaatc aaaactcttg cccagtcaca
       61 tttaacaaaa gaagaacaac atctaatcgt cgtatatctg gcagaaataa aagagttcta
      121 taaattaaat tctatctttg atttttttgg ggtagaaaat ggtggtgcgc aacatgggcg
      181 attattcttt catgactctg aaaacaattc atccagggag ttgactgaac tcttcagatt
      241 aaataaggca ttattaaatg atctaaatga gaaaattgag caggttgcta tcgaatatag
      301 caaagaaaat tatgattata tctggaacca gatcattttc tcctatttaa aattgctgga
      361 acataatcaa cattggcaga gtttttggga aaaagagtca caagttctgg atgaagccaa
      421 attaagtaaa gcaaaacatc tcgtttgcag tgcgatatgc tttaaactat tttctgtgct
      481 ctcggccaat tcgatagaaa tgatgagcca tccctctgtt aaagtcttta tcaaattgtc
      541 aaatagcatc aattttgaag atattcgaaa agaaatgcgt aatcgtttgc gtagtgaaat
      601 tttagaggat attaaaattg caactatgac aaaaaagaat tccaaactga catacaagtc
      661 attatatgta aaagtaaacc gggatgctca tttctgtgct ttgagcttgg aagagaaggc
      721 tttagctgta attgcaggag ttgcagatgc ctgttcggga aatgatgccc attttaaatt
      781 attaatggaa agtattgtga aagcaaaaaa gcaatttcct gtcgatgatt ttaatgcaaa
      841 agtccaagcg atacttgagg aattgcaagc taaaaacaag ctccctcaaa aatatgaaaa
      901 aaaatttcag gaaatttgtg aaactcaaga caataaaaga atattgaaaa aaaacttgtc
      961 gctaccacag cttgcttcag taggttcaac tttttatcat tctaaaaaaa gagagggttc
     1021 ttctgatgaa aatacaagtt cttatcctca tagaccctag gttacgcttg caatcgctct
     1081 tcatttccta tgtaccgcgg catgctcgcg gtatccaata actcattgtc agctacatgc
     1141 tttttcataa agatcgcatt attctggttt agtccttcga tcaaatttaa tttctactgt
     1201 ctgtactagt ttctgaagtt attttcacgt tcataggctt aaaacctata gagcgatgag
     1261 aaaaactatt tgtcgatttc actaggaagc aaattggaaa gttctgaatc agttagaaaa
     1321 tagactgatt ttacaaggtg ttttgcattc tgtgcacagg attatccaca gattttgtgg
     1381 ataaatatat ctgagagaat taaattaaaa aagccaggta acccttacat ggaaagggct
     1441 atataaattt caatagtgaa attagcaaga gttttgtgca attgattttt gaagttatcc
     1501 acagggataa gaaggaatga attttacttg ttgcccgctt atgttagcaa agaaaaaaat
     1561 aaaaaacagt cttgacttca ataattttaa ttattttttt aactttgcaa aaattgttca
     1621 aaattgccca gccatcgttg tgtaatttgt ctggcgattg ctgttttttc cagcaccagt
     1681 tgttttgcaa ttggacgtag aatagcgaag agggttttat cgcgttgtaa gttagctatt
     1741 ttaaattgtc taaagccagt ctgtctggtt cctaatattt ctcctgagcc tcttaactcc
     1801 agatcctttt cggagattag aaaaccgtct gttgttgaac gcatgatctt gagacgttca
     1861 gtaccttgtt gtgatagagg agattgataa agtaatagac aatgagattg attattacct
     1921 ctgcctacgc gccctcgtaa ttgatgtaac tgcgacaaac caagtcgctc tgcattttca
     1981 atgatcatta aactggcatt gggaacatct acaccaactt caataacagt agttgccacc
     2041 aataaatcaa tctcgccttg tttaaaggct gccatagtag cttctttttc aagtgctttc
     2101 attctcccat gaacaagacc aacacgtgcg aaagagagct gttcttgtag tttttttgag
     2161 gtatctgttg ctgccatgca ttgtaatttt tcagattctt ctataagggt acagacccag
     2221 taagcttgtt tgccgttggc gattgccgct tgcaaacgtt cgataatgag ttcacgctta
     2281 tcctggttta atactgctgt tgtaattggc atacggccag gtggtagttc atcgatgacc
     2341 gatatatcca aatgggcaaa atgagacatt gacagagttc ttggaatagg ggtggctgtc
     2401 attaataatt gatgagggat taattggttt aattgccctt tttgttgtag aaggagccgt
     2461 tgttctactc cgaatcggtg ttgttcgtca ataatgacca aacccaaacg agcgaattcg
     2521 acttgctcct gaaataaggc atgagttcca acaatgagct gacagctatt gtcctgcagt
     2581 gctgcaagcg tattttttcg ttctgtggtt ttcattttac cgctcagtct taatacatta
     2641 ataccgatag gttctaacca tttaccaagg ctatttgtat gttgttcact taataaatcg
     2701 gttggtgcca taaaagcaac ctgaaatcct tgtgaaatgg cttgaagggc ggctagtgcc
     2761 gcgataatgg ttttacccgc cccaacatct ccttgcagca aacgaagcat gggtttgctt
     2821 tgagttaaat caacgctgat ttctttaaaa acccgttgtt gtgcgtttgt aagactgaat
     2881 ggcaatgatt cgataaaacg tttatgaatt gcattatcga aaaatatagc aggagcttgg
     2941 agcttgctac gtgactgtct tgcaaactgc atactgagct gttgtgccag taattcatca
     3001 aatattaacc gcttgagagc aggatgttcg ccggcttcga ggttggagag ggagatatct
     3061 ggcggggggt tatgcagtaa tttaatggct tcgcccaaat cataaaaatt gttttcctgt
     3121 aattgttttt cactcatcca ctccaattga tgaagttcgt gttcagattg ttccagggct
     3181 attttaacta actgccttaa tcgggtttga gtcaagcctt gagtcgatgg ataaattggc
     3241 gtaagcgttt cttctacgtg gaaatctgat tcttggtcaa ttaattgata ttctggatga
     3301 atcatctcca actggttgtt aaatccgcgg acttcgccaa aagctcgaat catggcgcta
     3361 ttattcaagg cttgaatttg ctgcttattg aaatgaaaaa aacgaagctt aactactcct
     3421 gttttatctt ccacataaca attcaacatc atccttttgc cgtattttat ttcagttttg
     3481 catacatgcc ccgcgataac gcaccattca ttggagcgca aatcctgaat tggtgttatc
     3541 ctggttctgt cctgatattt ataaggcagg tggaaaagca agtcttgtat cgtgaaaatt
     3601 ccacatttag caagttttgc tgcaatggtt gggccgacgc cagttaaata ttcacaagag
     3661 ttactcagca cggtattatt taagattaaa aatttaataa taacaggaaa atatattcgt
     3721 tgcgacttga gtcatcattc tggcaatttt gcccaaatca ttactaagtt acttgttgct
     3781 tgcgagtatc tattcatcaa agatggagtg taattggatg aatcgtatta taacgttcac
     3841 agcgggtttg atactggttt ggataacagg ttatttatta atcgcaggaa gaggactatt
     3901 aattccaatt gttatatcca tttttgtctg gcacctgttg aataccatta atagcggtgt
     3961 tcagaaaatt ccttttattg gcacacatct cccatcctgg ataaggatga ttttatcttt
     4021 gtttgttgtt gtttgcctcg taatggttct tattaacatc attaccaata atgtcagtga
     4081 tgtgatagcc gcttctccca ggtatcagga aaatttactg cacatattta acagcctgga
     4141 tcaacgattc catgttaagg gtatttttga tttcgacagt ttaattcaag ggttaagcat
     4201 tcagagtatg cttgttaata tttatggggt ttttactaca ataaccagtt ccgctgttct
     4261 gatctcctta tatgttgtgt ttctgtttgt tgagcagcat tattttactc aaaaaatgga
     4321 tgcctttttc cctcaggcag agcacaggaa gctggttaaa aacattatct ctcatattac
     4381 aaaagatact caaacttatc taggtattaa aactttgttg agcctgctca ctgccacgtt
     4441 gagttggttt attatgaaat gggttggatt ggattttgcc gagttttggg cattattaat
     4501 tttcttttta aattatatcc ccaatatagg agctattgta gcaacagctt ttccagctgc
     4561 gcttgctttg atccaatttg aaagctggtg gccgtttttg gaagtgactt ccgggatagt
     4621 ggctatacag tttttcgtgg gcaattttct agagcctcgt ctattaggta aatcactaaa
     4681 cttaagtcct ttagtgatat tgtttgcctt agcactatgg ggtgccattt ggggggtact
     4741 aggcatgttt ttgtctgtgc ctatcactgt aatgatgatg attatttttg cgcactttga
     4801 tgtgacaaga cccatagcaa ttctgctgtc tcaagatgga tatattcaca aatcctatga
     4861 atctcttgaa aattaatgag ctattgctgt gtctattgtt gtagtgatgg tgcttcgggg
     4921 ttgagagcct ttccatatgg tatgaaaaag agcataatat cggtattttt gacggataaa
     4981 tgagaagtat gaaagctata atcgaatatc ttttgaaaca ggcattaatt aatctgcaac
     5041 aatcaggcga aatgccaatt gatttggaga ttgaaataaa agttgagaat gccaaagatc
     5101 cagctcatgg tgattatgcg actaatttgg cactggtatt agctaaacca tgccgacaag
     5161 cacctaaagt attagccgag cgattggttg cagtaatccc cgcagatccg tctgtagaga
     5221 aaattgagat agccggggca ggatttatta attttttcat gcgcagcacg gcacgctcgc
     5281 tcatcatttc tgaaattttg aataaaggca aggagtttgg ccaaggtaat ttaggacaga
     5341 gccaaaaagt attgattgaa tttgtttctg ccaatccaac agggccttta catgtaggac
     5401 atggaagagg ggctgctttc ggagcaactt tgggtaatgt tttaaaagcc gctggttacg
     5461 atgtgacttt ggaatattat gtcaatgatg cgggcaggca aatgaatatt ctcgctgtga
     5521 gtgtgtggct gcgctacctc gaattggcag gtgagccaat tgcttttccg gcgaatggct
     5581 ataaaggtca atatgtttat gagattgcac aagagatgtg gtcagagcag ggtaatcaat
     5641 tcgtgcatcc atggatttcg gtagtagaca atttacctgc tgatgaaccc gaaggtggag
     5701 ataaagagac ttatatcgat gcgatcatcg cgcgagcgca atccttactg ggcaaggatg
     5761 ggtttgctaa cttccatcaa catgctttaa aaacggtatt ggatgatata aaagatgatt
     5821 tgcaggcgtt tggtgttcgt tttgacagtt ggttttctga gcaatcgttg tttgaagatg
     5881 gttctattgg gaaaggtatt caggccttga aagatagagg ccacacttat gaaagagaag
     5941 gcgctttatg gtttagggca actgattttg gtgatgaaaa agatagagta ttagtccggg
     6001 ctaatgggca aacgacatat ttcgcttctg atgttgctta tcactggaat aaatatgata
     6061 gaggttttga tcgtgtcatt gatatttttg gtgctgatca tcatggttat gtcacgagaa
     6121 ttaaaacagc tgttaaggca ttaggacatg atgaaagtgc attggatgtc attttggtgc
     6181 agtttgccat tctatatcga ggtggtgaca gggtgcaaat gtctactcgc agtggttcat
     6241 ttgttacttt gagagagcta agagaagaag taggaaatga tgcggctcgt tatttttatg
     6301 tggcaagaaa acctgaacaa cacatggatt ttgacctgga cctggcaaag tccgaatcca
     6361 gtgacaatcc tgtgtattat attcaatacg cacatgcccg tatctgtagt gtattgagac
     6421 agttaaaaga aaggagtttg aagtgggata aggatatggg acttaaaaac cttgatttat
     6481 tggagcaaca acatgagacc acattaattt ctctgattgc ccgatatccc gaagtcattc
     6541 aatctgctgc cgcttcttgc gagccgcatc agcttgctta ctatttaaga gagctagcca
     6601 atgggttaca cagttattat aatgcgattc agctattatg cgaacaagaa caattgcgat
     6661 gtgcccgttt gtgtttgctg gagtcggttc gacaggtttt aaataatggt ttggctatat
     6721 taggcgtatc agctcctgag agtatgtgat ggcaaaagat tatggaaata ggcgtgctgc
     6781 acgtcgcaac agaggaccgc accagttttt ggttattatt gtgacttttt tgtttggata
     6841 tataaccgct tcgtttctcg atgtgcaaac gataagtcaa tgggtgaata cacaggtttt
     6901 agctcaacat gatgatgaaa aagcgccaac taaagtggcg caagaacata aacaagtgcc
     6961 tcctaagcct aaatttgagt tttatacatt attagcgaat gaaaggggac cggcatctca
     7021 ggcatctagt cagcaaaata cagacaatca tgcggcagat aagacatcaa ctccaataca
     7081 aaagactgca aatactgtca ctgctgctgt aaataactca gtgaaatcaa cgatagctaa
     7141 tccaacaccg caaaccccag tgaaggtagc ggaggcaaaa ccaacaacca tgcaacaacc
     7201 taataaagga gcatatttgg ttcaagtcgc atcttttaaa gcgcgtcagg atgcagaaca
     7261 aatgaaagga atgctaatcc ttaagggttt tgatgtcagc gtggttccag tcacccaagc
     7321 ccagggaaat tggttcaggg tagtggtggg gccctatcca aataaggcct tggctcaaaa
     7381 agctcaaatg aatcttgcca aaacagagcg cctgaacggc atggtgcgca gcgttggcgg
     7441 ataaggggta atcatttaat caaagaagat tgcttcttgc agattatctg tttacaacca
     7501 cttggattcc cgcagcgttt tttacagcat ggggctttgt aacagcgacg gaaaccttag
     7561 tgacaggata cttttgcttg attaattgtg caatttgttc cgctaccgtt tcaattaatt
     7621 gaaacgaagt tgactctaca aattgggtta ctgtctggca caaatcagcg taatccagtg
     7681 tattagacaa attatcgttg caatctttaa agtcaaaggg aatggatata tcaattaata
     7741 atcgttgatt aatacgctgc tcccacccat aaacaccaat ttttgtttca acactgagag
     7801 ctgtaatatt taaagtatcc atttgatatc cattaataat ttatcaggac ttatgaaaaa
     7861 tcctcaatct ctttgtttaa aatttttaaa atcattcatt taatttatct aaattttctc
     7921 atgaaaacat tgtttgcttt gatcttgggg tttttcgtgt gtcctgttta tattgttaag
     7981 tatcgattgc tatttatacc actgtattga ttaccttaac atgatactat aaacactttt
     8041 tattgtgata gtgattaaat gatgtttgat ttgaaccaat ccctttttct tcgagctttg
     8101 agaagacaac ctgtggagag aactccaatc tggattatgc gccaggctgg gcgttatttg
     8161 cctgaatata gaaaagtaag agagcatgct ggtgattttt taaatctttg caagaatccg
     8221 gaactcgcct gtgaagtaac cttgcaaccg ttgcgtcgct atgctttgga tgcagctatt
     8281 ttattttccg atattttaac aatacctgat gctatggggc tagggctcta ttttgctgaa
     8341 ggagaagggc ctcgtttcac aaatccttta caagatacca aggcaattca taccctgaaa
     8401 attccttcca ttcctgaatc tttatcctat gtctttgatg ctgcgcgctt aatccgccaa
     8461 gaaatgccta aagaattacc tttgatcgga ttttctggta gcccatggac tttagcttgt
     8521 tacatggttg agggcgggag cagtagagat tttaagcgaa ttttaaattt aatatacact
     8581 gaaaaagaag cagcgcacct gttattgagt aaattggctg cctctgtgac tgcctattta
     8641 attgagcaaa taaaggcggg ggttaatgcg gttatgatct ttgatacgtg ggggggagtt
     8701 ttaactccac aaaattataa ggatttttct ttggcatata tgcatcaaat tgttcagcaa
     8761 ttgaaaaaag aataccctga tatccctgta attttattta ccaagaatgg tgggcaatgg
     8821 ttagaatgga tggcagaaac tggttgtgat gcattaggag ttgactggac gtgtgatttg
     8881 gcttccgcgc gcaagagagt aggaggaaaa gtcgctttac aaggaaatct ggaccctgct
     8941 gttttactaa ctacaaaaaa ttgtatacga agtgaggttg ggagtgtact ggcatcttat
     9001 ggttatggaa cagggcatat ctttaatttg ggtcatggca ttactccgga tgtaccccct
     9061 gagaatgttg caataatgat tgaggcggta catgagataa gtcctcaata tcatttgtag
     9121 tttgtgcgag gcaaaaatta ttgtttcaaa atagtgaaat gcctcaagaa taagacaatc
     9181 gtaacagtac acggggtatg caaatttttc tgtttataag cctatttatt tgagttgtct
     9241 ttcccgcgaa ggcgggaagc taactaaaat caacccttgt gctagcttta tggatagatt
     9301 gctgctttcg cgggaaagac agaggagcta atattcctta attacatacc ccatggacag
     9361 ttacagataa tcagcttttg gcatagatac tggccttcat tcaagaggtc atgaatttca
     9421 ttaagattca tttctgaatt gatggcttag ctgaatagcc atttccagag attgttcata
     9481 gtttaatctt ggatcaacca ggctgtgata ggcagttttg aggtcatgag gagctaatcc
     9541 acgagcgccc ccgatacact ctgttacatt gtcaccagtt agctcaaaat ggacacctcc
     9601 gaggtagcta cccatgctgc gatgaatttc caaagcttgt tttaattccg ataaaatgtt
     9661 atcgaagtga cgtgttttag taccgtcttc tgttgtttcg gtattgccat gcatagggtc
     9721 acatgaccac gtgactggaa ttttgctttt ccttactgtg tcaatcagtg gaggtaacaa
     9781 ccggtcgata agttttgctc ccaggcgagt ataaagtaaa actcgtcctt cttccttttg
     9841 cggattggct atgcttaata cctccgataa ccattcaggt gtagctgctg ggcctatctt
     9901 aatgcctatt ggattttgta cccctcgtag aaactcaaga tgtgcactgt ccgtttgtgc
     9961 cgtacgcatt ccaatccagg gtaaatgagt tgaaagattg taccattttc catctttcaa
    10021 ctgtcttgtt aatgcttgct cataatgtaa atgcaacgct tcatgagagg tgtaaaaatc
    10081 aactttactt atgctactcg aacgtattcc atctatggca ttcaaaaaat ccagggcgtc
    10141 ttcaatagag tctacaatat gttggtattc gttcttctga ggggagtgct caacaaaacc
    10201 taaatcccag cgttgagggt gatgcagatc agcaaaaccg ccatctagca gcgctcggat
    10261 aaaatttaaa gtcatggcag aacaactgta tgcctgaagt aataatttag gattgggttc
    10321 cctggcagtg gcagtaaatt caggtgaatt aacgatatct ccacgataac tgggtaaagt
    10381 aactccattt atggtttcgt aatctgagga gcgtggtttt gcatattgcc cagctattct
    10441 tccgatacga ataattggtt tacgcaagcc atataataag attaggctca tttgaagaat
    10501 gatttttaat ttgttactta tgacttctga acgacagtca ttaaaagatt cagcacaatc
    10561 accaccctgc agaataaaag cattaccccg gccagcctgg gcaatttcat tttttaaatg
    10621 tttgatttca ccgctggtaa ctaatggggg cagcaaactt aattgttcta ccactttatt
    10681 taattgctct tcatcagcgt atgaagctgc ctgcaaatac gaatatttct gccatgtagt
    10741 tggcgaccat tcttgcataa tttacccaag ttatttttgc tttagttgtc gtttagactt
    10801 aatttaactc aaaggattat taaatacttt gattttttta cctaaatgag aattaagaca
    10861 gattttttag ctatctaagt atggaataaa tggacaatta ctgcaagcca tgatttgaaa
    10921 agtttcaatc agttaatgac ctgaatttta aaccaagatt taaaatccta acaggggctt
    10981 gaaaattgca aatacaatcc ccatttgtag gaatcgagtt agtcaggagc gattttgatg
    11041 agtaaacaaa ataaaaaaga ttggaaaaaa tttagagacg aacacaaaga agaacacaag
    11101 gtagagaatg agattctgga agaagaaacc gatgaagaat cacaacatca agaacctgct
    11161 ttaggacatc ccagctatac tgccttagaa gagcaactga cattggcgga acaaaaagca
    11221 catgaaaatt gggaaaagtc tgtacgcgcc cttgctgaat tggagaacgt tcgtcgtcgt
    11281 atggaacgag aagttgccaa tgctcataaa tatggcgtag aaaaactgat ttcagcttta
    11341 ttgcctgtag tggacagtct tgagcaagca ttacaattag cggataagaa cagtgatcca
    11401 tctatgcatg aagggcttga gttaactatg aaactctttc ttgacgcatt acagaagttt
    11461 gatgtggaac aaattgatcc tcttggacag acatttgacc ctcaacagca tgaggcaatg
    11521 tcaatgcaac ctgccccagg agcaccaccg aattctgtaa ttactgtatt ccaaaaaggt
    11581 tacaaattga gcgatcgagt aattcggcca gctcgggtga tagtttccac caaataatct
    11641 ggtgtttcag ttgaggtact tttaaacaaa tttttagatg cagtgtattc agcttttttt
    11701 agggtgatag cttgaaatcg tcatgatgca ccccatatgt ggaatattgc atgagaaaat
    11761 attaatttgg agcaaaatag aatggctaaa ataataggta tagacctggg tactacgaac
    11821 tcttgtgttg ctgtcatgga aggtgacaaa ccgaaggtaa ttgaaaatag tgaaggtcac
    11881 agaacaactc cttctatcgt agcatttact gatgacaatg aaatattggt aggccaatca
    11941 gccaagcgtc aatccgtgac taacccagag aaaacactgt tcgcgattaa acgattaatt
    12001 gggcgtcgat ttgatgatcc tattgtgcaa aaagatatta agatggttcc ctacaaaatt
    12061 atgaaagcgg acaatgggga tgcttgggtt agagtcaagg atcaagataa agcaccacca
    12121 caaatttctg ctgaagtgct aagaaaaatg aaaaaaacag cagaggatta tttgggtgaa
    12181 gaagtaaaag aagcagtgat tactgtacct gcctatttca acgattcgca acgccaggca
    12241 accaaagatg ctggtcgtat tgctggcctg gaagtaaaac gaatcattaa tgagcctacc
    12301 gctgctgcac tggcttatgg tatggataaa aaacgcggtg actctgttat tgcggtatat
    12361 gatttagggg ggggaacttt tgatatttca attattgaaa ttgccgaagt ggatggcgaa
    12421 catcaatttg aagtacttgc aaccaatggt gataccttcc tcggtggaga agattttgat
    12481 ttggctttaa ttgagtattt ggcttctgag ttcaagaaag acactggtat tgatttgcat
    12541 aatgatcctc ttgctctgca acgcttgaaa gaggctgctg agaaagcaaa aattgaattg
    12601 tcatctgcac aacaaactga tgtcaatttg ccttatatta cagcagatgc ttctggacca
    12661 aaacatttaa atatcaaatt gactcgcgca aaactggagt ctttagtgga gaaactggtt
    12721 gagagaacta ttgagccatg taaaacagct ttaaaagatg caggacttac tgtctcacaa
    12781 atcaacgaag ttattctggt cggtgggcaa actcgtatgc ctctggtaca aaaaactgtg
    12841 gaagaattct ttggcaaaga acctcgtaag gatgtaaacc ctgatgaggc tgtggcagta
    12901 ggtgctgcaa ttcaggcagc agtattgtcc ggtgaggtga aagacattct tttactcgat
    12961 gtcactcctt tgtctctcgg tatagaaacc atgggtggtg taatgaccaa actcattgaa
    13021 aaaaacacaa ctattcctac aaaggctaca caagtgtttt caacagccga tgataatcag
    13081 acagcagtga ctgtgcatgt attgcagggt gaaagagaac aggcttcagc aaacaagtca
    13141 ctgggacggt ttgatttgcg tgacatacca cctgctcctc gtggtgttcc tcaaattgaa
    13201 gtaacttttg atattgatgc caatggtatc ttgaatgttt ctgccaaaga caaagcaact
    13261 ggcaaggcac aatccattgt catcaaagct tccagtggtt taagtgaaga agaagtcgct
    13321 gctatggtga aagatgccca atcgcatgct gaagaggata agaaattcaa ggaaatggca
    13381 gaactgcgca atcaggcaga tagtttgatt catagctgtg aaaaatccat gaaagatttg
    13441 gctgatgagt tgtctgagga tgagaaaaaa ggcatcgaaa cagcaatttc tgagctgaaa
    13501 gaggcggtgc aaggaactga caaagctcgt attgaagaca agctgaaagt attgacagac
    13561 gcttccgcaa aaatggctga acgtatttat gctaaaaaat cgtctgaagg tcaggcagct
    13621 caggggcaaa cacaatctca ggaatctaca aaacccgcgg aagagggagt tgttgatgct
    13681 gagtttgaag aagttaaaga agaggataaa aagtaattta atattactct cgccctctga
    13741 tttaagaggg cggaagaaga attcatcggg ttataataaa taaattttgg gtcggggcga
    13801 tattatcgtc ccgtttgtct attgaatagc acttaagcta atttagagtc ctcatacgac
    13861 tgagagaatt aaaattaagt gattgttata aattgtgagc agttatggaa cagcgggatt
    13921 attatgaact tttagaagta agtcgaaatg cgagtgatgc agagattaaa aaagcatatc
    13981 gcaggctggc aatgaaatac catcccgatc ggaatccagg tgatacctct gccgaagaaa
    14041 aatttaaaga aatccaaaaa gcctataata tcttgtccga taagcaaaaa cgcgctgctt
    14101 atgatcaatt cggccatgcc ggagtggatc cttcaatggg tggtggccca ggtggttttg
    14161 gcggatttgg cggatttggt gacgtttttg aagatatttt tgagaatatt ttttcagggg
    14221 ggcgtgggca tgggcgtcaa tcacgtggac aaagaggcgc tgatttgcaa tttaatgtgc
    14281 aattgaccct tgaagaagcg gctattggca aagaagttga aattaccgtt ccaagacatg
    14341 gtacttgtac tgtttgtgag ggatcggggg ctaaaaaagg cacaagccca aaaacttgtg
    14401 aaacatgcca gggcatgggg caagtcagaa ttcaacaagg ttttttttcc atacaacaaa
    14461 cctgtcccac ttgtcatggt gagggaaaaa taatttctga tccttgtgcc agttgtcatg
    14521 gtcaaggaag agttagagaa agtaaaaaaa taaatgtcaa aatcccggct ggagtcgata
    14581 atggtgatcg ggtccgttta agtggagaag gtgaagcagg ggtgcatggt ggtggttcag
    14641 gagatttata tgttcaaatt agcttgaaaa aacatgctat ttttgagcgc catgaaaatg
    14701 atttgcattg tgaagtacct attagttttg caactgcggc cttaggagga tcgatcgagg
    14761 tacctacttt ggaaggccga gtcactttaa aaattccggc ggaaactcaa acaggcaaag
    14821 tattccgctt gagaagtaaa gggatgaaat ctgtcagagg atatggacaa ggcgatttac
    14881 tatgcaaggt agttgtagaa acaccggtca atttatctcg agagcaaaaa gaactgttaa
    14941 ataagctaca ggattcatta gagaatgcca agggaacgca ctcacctaaa acaagctcct
    15001 ggtttgctgg tgtgaaaaaa ttctttgaag acatgaaatt ttgacttaat taagttacaa
    15061 ttggaagatt tgtcgttttc agcatggatg cctattttac gctggataag catactttga
    15121 acataattga gtatattcat gattaataaa tcagcaattt tagctttagc tgatggcact
    15181 gtttatgagg gcctgtcagt aggggccatt ggtgagtctg ttggtgagtt ggtttttaac
    15241 acgtcaatga ctggctatca ggaaatgctt actgatcctt cgtatgccag acaaataatt
    15301 accttaacta ctgctcatgt gggaaatacg ggttgtaaca tggaggacat ggagtccaca
    15361 aaagtctggg cagctggctt ggttatgaga aactgcactg ctgttccaag taacttcaga
    15421 gctgaacaat ccttcccaga ctggttaagt aaaaatggag tagttgccat ttctggcatt
    15481 gatacgcgcg cgcttacatt gaaactgcgt gagaagggct ctatcggagc ttgtattagc
    15541 accaatgtgg agaaacctga aattgctgtg gcaaaggcta aatcctttgc agggcttcag
    15601 ggtgcggatt tggctttaga ggttacaaga caaacgatag agcgttggca tgaaggtcgg
    15661 ggaatgtgga gtctagacaa acagcctcaa cagcttcatg ttgttgccta tgattttggt
    15721 gtgaaacata atattttacg tatcttacat gacaagggat gccatattac tatagtccct
    15781 gctaaaactt cagcagaaga ggttttagcc atgaatccta atggtgtctt tttatccaat
    15841 ggaccgggtg atccccaagc atgcgattat gccattaaag caacgcaggt atttttagac
    15901 aataatattc ctttatttgg tatatgtctt gggtttcaga ttttggcttt ggcatgtggt
    15961 ggtattacta aaaaaatgaa atttggacat catggttcca atcatcctgt agttgaaaca
    16021 actggtaaaa aacgtgtttt tataacaagt caaaatcatg gttttgctgt agatgaggat
    16081 tcattgccca attgcctgga agttacacat cgttcattat ttgataacag tttacaggga
    16141 ataaggcaca aaacaaaacc ggcatttggt tttcaaggcc atccagaagc aagccctggg
    16201 ccacatgata tagatatttt atttaatgaa tttgtaaatc tgatgaaata aatttttagc
    16261 ttcagaatta ttattttggc aagagtggct tgttttaagc ttgcttacaa tgattgtaat
    16321 acccctaaat gctatccatg gctattaaag attagtaaaa tgacatggat ttaccaaaac
    16381 aatgacttta acttgttttg tgttttaagc agagataaag attagggata ctaaaaatag
    16441 aaaatacaga gggtatgtaa tgaatgaagt ctatgttttt acctcagaat ctgtttccga
    16501 gggccatcct gataagatag ctgatcaaat ttcagatgcc attttagatg ctattttagc
    16561 tcaagaccca aaagcacgag tggcttgtga ggtattggtt aaaaccggta tggttttggt
    16621 tggtggagaa attacaacaa aagcctgggt agatgttgaa gatataactc gtcatgttat
    16681 caaggacatt ggctataata gttcccagat ggggtttgat tgggaatcct gtgctgtgct
    16741 ttcagcgatt ggcaagcaat caccagatat agctcaagga gtagataatc agcaaactaa
    16801 aattctgggt gctggcgatc agggacttat gtttggttat gccagccgtg aaacagatgt
    16861 ttttatgcct gcccctattg cctatgcgca tcgtttgatg gagaaattag ctaaggctcg
    16921 taaatcaggc caattgccct ggttaagacc tgatgcaaaa tgtcaactta ctttgaaata
    16981 tgaacaaggg atgccggttg aagtggatac tgttgttttt tctacgcagc attcacctga
    17041 tattgagcat aaagatttgg ttgaggcgat ccgtgaagaa attattaaat cagttcttcc
    17101 tgctgaatgg ctcaatgata agacccgata tttcattaat ccaacaggac gcttcgtaat
    17161 tggtggtcct ttgggcgatt gtggcttaac tggccgtaaa atcattgttg atacttatgg
    17221 tggtatggca agacatggcg gaggctgttt ttcaggtaaa gatccttcga aggtagatcg
    17281 ctccgcagca tacgccgcaa gacatgttgc caagaatatt gtggcggctg gtttagcgga
    17341 taaatgcgag ttacagattt cctatgctat tggtgttgct gaacctacat ctatttttgt
    17401 tgatactttt ggtactggac gactaaaaaa cagcgaaata attgatttaa ttcatacgca
    17461 ttttgattta acgccgcaag gtattattga tcaacacgat ttgcttcgtc ctatttatag
    17521 gcagacggca acttatgggc actatggcag ggaaagtttt ccatgggagc gattggacaa
    17581 agtagctgaa ttaagcaagg cattataatt ttttaaggat actctaatgg aactggtaga
    17641 agaaggagta cagaaaatca atacgttaca agattataaa gtagcagata tttcattagc
    17701 ggactggggg cgaaaagaaa tagcgattgc tgaaaccgaa atgccgggtt taatggcttt
    17761 acgcgaggaa ttttctgcga aaaaaccact tcaaggcgct cggattgcag gttgtctgca
    17821 tatgacgatt caaaccgcgg tattgattga gactctggtt gcattgggtg ctgaagtacg
    17881 ctggtcttcc tgcaatattt tttctactca ggatcatgca gcttccgcta ttgctgcaaa
    17941 aggaatccct gtttttgcct ggaagggtga aacagaagaa gaatattggt ggtgtgttga
    18001 gcaaacttta agtggcccca atggttggac acctaattta ttattagatg atggtgggga
    18061 tctgacacaa attgttcacc agaaacatcc caaattactg accgcaatta agggagtatc
    18121 tgaggaaacc actacaggtg tagccaggct gtacgaaatg gcgaaacatg gacaattaaa
    18181 aattccagcc attaatgtta ataatgcggt gaccaaatcc aaatttgata atttgtatgg
    18241 atgtcgggaa tccctgcttg atgggttaaa acgtgcaaca gatgttatga tagctggtaa
    18301 ggttgccttg attctaggct atggtgacgt agggaaaggt tgcgctcagg ccttgcgcgg
    18361 acagggagct acagtacttg tcgcagaaat tgatcctatt tgcgcacttc aggcagcaat
    18421 ggaaggctac agagtagtca ctctggatga tgttgctgaa caagtggata tcgtagttac
    18481 agctactggc aattaccatg ttgtaactca cgatcacatg aaacgtatgc gcaatcaggc
    18541 gatattgtgc aacataggcc attttgattc cgaaattgat attcaaagct taaaacagta
    18601 tcaatgggaa aatatcaagc cgcaggttga tcatgtgatt tttcctgatg gaaagcggat
    18661 tattatccta gctgaagggc gtttagtgaa tttgggctgc gctacaggcc atccgagctt
    18721 tgtcatgtct gcttccttta ccaatcaagt attagcgcag attgagctgt tccaaaactc
    18781 tagccaatac cagaatcaag tatatgtact accaaaagtt ttggatgaaa aagtagcccg
    18841 tttgcatttg ggacgtattg gtgcaaaatt aactcaatta agtaatgagc aggctgatta
    18901 tatcggcgtt gataaaaatg gaccttacaa gccggatcat tatcgttatt aaactaattt
    18961 tattcaagat ttagtcaaga taactaaatc ttgaattttt ttacattatt ggatcttata
    19021 ctctaatata gattagaatt aattcacttc tcacttaaaa aagaaaagcc tgttgatgga
    19081 tctggttata gcggcaaatt atctttctac tctggaaaaa tctcttgaac aatttgggat
    19141 taaccatttt ttagaaaaaa ttggctttga tctgataaga gtacatgatc ctgatgccta
    19201 cttaactcag gatgaattta accaggttat tatggaagct tataaattaa gcaattgccc
    19261 ggaattgggt ttggtttttg gtcaaaactt atctatagtc aatcacggtt ttctggggta
    19321 tgcagcaatg agcagtccta catttggaac tgctatacag actgtgctca gttaccttaa
    19381 tacacgaacc aatttgttgt ctctggagtt acgccagttt aataatgaaa acagtgctta
    19441 tatcaagtta ttaccacaca caaaagaaat ggttttatca cgttttataa ctgaattggc
    19501 agttgctcat cttattaaaa tgagaaattt tttgattagt tgtaatgacc cattgctcaa
    19561 gattgagtta aattacgatg aacctcctta tgcaaaatat tatcaaacaa tttttcagac
    19621 taaaattgtt tttaatacag aggctactcg cgtttggttt aaagcggagg aactgaatta
    19681 tcccatccat tttgctgacg atgtcagtta tcagctggca aaaaagcaat tgcaagaact
    19741 tgaaaaaaaa atgtcattaa aagaggatat ggcaagcaag atcaaatcta ttttaatcaa
    19801 tcagaattta aatcatgcca gcatggatga gatagccagt cgattatgta tgacttctcg
    19861 tactctaaga cggcatttgc agagattgga tgtgacttat caagagttgc ttgatgaggt
    19921 aagagaacaa aaagcaaaga catttttgct caataataat atttccatga ccgagatcag
    19981 ctttttattg ggttttcaag atacatccaa tttttcaaaa gcattcaaac gatggacagg
    20041 aatcactccc acagaatata gggaaaaaca tgaagtccaa tgattgctcc attttaattt
    20101 aatctcatgg cttattgatt cttgagttga tatttattct cctgcatact gaggtggttt
    20161 tatttttaaa aattggtcaa ggctacggat tgactttaaa aaggagattg aagtatgggt
    20221 ttgaaatcag gaatattggg attaagtctt ttatcgcttg cttgtggcag ttttgctatg
    20281 actgaaactg aaacaatcag ggttatcatt aaatataaac agcaacccgc cactattgct
    20341 tcactgaaat cacaagccat taaatctgtt caaatacccg ttaaagaaat gaagcctatg
    20401 gccaatggcg cttattcgct tatttttgat acatcgaatt cccatgctat tgtcaaggct
    20461 aaagaagaag atgttacagc caaagtttta gaacgtttgc gcaaaaatcc caatgtactt
    20521 tatgcagtta aagacagaat aggacatttt aagcctctcc ctgatccagt aataaaccag
    20581 gagttaagtc aattgttatc ccatgagagt caatgggatg aattcgaaag gccagcggga
    20641 attcgtttgg aatccaggcc tggtttaaga gatggcgcat gggcatatac tacaggaaag
    20701 tccaggaagc cgatagttgt tgcagtttta gatactggga ttgcattgaa tgacagtttg
    20761 gtgaataacc ttgtaaaaga taatgagggt aatgtatttg gatggaattt tgccgggaat
    20821 aacaatgatt tatctgatga aaccagatct taccatggca cccatgttgc cggtaccatt
    20881 gccggttatg gcgatgtcat gaacggggtt ggtgaggatt taaaaattct gactgttaaa
    20941 attcctgccg agaatggtat gttttatgaa agtgctgtgg tgaatagtat ctattgggct
    21001 gttggaggtg atgttccagg agttcctaaa aatatacatc cagccaaggt attaaatatg
    21061 agttttggtg tcgatttaga gcctggaaaa gaaattgatt attgcgacga agctttacaa
    21121 gaggctttat tctttgctcg caagaaaggg gctgtagtcg cggttgctgc cggaaatgat
    21181 aatgtttggg aacactataa tgcgcctgca gtgtgcaatg gaacgatcaa ggttgcttcc
    21241 accggaccag aaggattgcg ttcgtacttt tcaaattatg ggcccagtat aacccttgca
    21301 gcaccgggag gtgataagcg ttatggcact tcgggaggta ttttatctac cgtaaacccg
    21361 ggaggtggat acaattcatc aggctttgat ttttatcaag ggacgagtat ggcttcgcct
    21421 catgtagctg gggtagcagg tttaattttt gctgtcagta ataagacttt gaagcctgaa
    21481 caagtagaac aaattttata tgttactacg catgagtttg ggcaaagcaa cgacaccaat
    21541 aaatcttgtg tcggcaaaaa accttgtgga catggaatat tggatgcgga taatgctgtg
    21601 caggcagcat taaatggtta tgatgtcttg ttctcagcgc ctaaaatgat tgctctggca
    21661 acgcatgaat gtggcgagaa tagattaaaa cctggtaaaa gcatcattaa tgacagtact
    21721 gcgacctgga ttcaagctaa gtcaagctgt gaaactggtg taagctatca gaagccacgt
    21781 attgaaaaag ccaaggatgg aaagatttat gcttattatg gttccgtaag ctatgaattg
    21841 gatcaatcgg cttataaaga atgtcgtgtc gttggatatg atggcgtagg ctgttattta
    21901 taatattaaa tgaaactcga ttctcagttt taagacgttt ttgatgaggg aagcattcag
    21961 agccaattta ctggttactt atggaatcct tctctcatca aattaaaatc cctgcattcg
    22021 ctcaggtatt aaaattttaa caaagacatc agaaaattac tcaagccaat tatttaatat
    22081 gtcaacaatt tttttggctt gttcagtact tgaaatattg aatttggatt tttgccaata
    22141 ttgattgtta cgtttctgat aacggcgtat agaatatttt acaggaccgt aatcattttt
    22201 tgcgttatcc caatcctggt atttaaacat aatagtggtc catgctcctt tggataggat
    22261 ttgtttatct agttccctgg ttgtttccac tccattttcg ttaaaagcaa ttgttatatc
    22321 atctatggtt tcagccattt gtcatacttc ctcaattatt tccgatggat tgtaatttgc
    22381 cgtcgacaaa agtcaaggaa aaggctggtt gatatggacc tggtttataa atccagattt
    22441 gtggttttgt ctctgtggga accacttggt taatgtaagt gttattgacc agatttggac
    22501 tgccgcaagc gccataaact ttagtggcgg gatctccgat ttgaatgctt gcgttgccac
    22561 acaaggaaaa agcgttagta ttgccgccat tcactcgaat atcctggact ttattattga
    22621 gaatatcgac ttccaattgc acgccactac tacctgtagg tatattccat acgccataaa
    22681 aagcggtacc ttccggtaca tttaatgatc ctgaatacag gccatcggag tttcccagat
    22741 tattataaat tagttgctgc acagggattt tctgcaaaat aggctgattg gactcttgtt
    22801 tactcagtgg ttgcccgcac gcagcaatga cctgatccat ggacataccg atattgatat
    22861 aaccatgatt ctgagggcaa tacaaagatt gtgcgctcat taaacggaat ggcataatta
    22921 gcaataataa actcataagt aatatcgatt tcatagcttt ttccattaat ccgtatcaac
    22981 taatagtata gttctgtttg tttatttttg cttttataat aacttccagg gcaaagaagc
    23041 gcttagaaac agggtaataa tttttaatat caccatgata ataaaaggtg agaaatcaaa
    23101 acctgaaatg tccgggacta taattctgcc tagtcgtaaa attggtgttg ttattacacg
    23161 caagaaatcc ccaagcggat gttgccattt gggattaaca taactcatta ttactcgaat
    23221 cagaacagca aaaaataata tttcgcaagg ttgaattatc aaatcaacaa gtatgtagag
    23281 aaaaataaag aggaatggaa tgattgcatg atacatcagt aaactcagtg aaattatttt
    23341 aaaaaattcc accaggatta aaactccaaa tgctatccag tcgtattttt gtcctggttg
    23401 caatggcttt tttataatcc aatacaaggg tttcactaga ggatcagtaa tggtatggat
    23461 taattgagag aatggattaa tagcactgat acgaaaaaat cgtatagcaa ttctaagcca
    23521 caaactgaaa ataacgatag taaataataa ggataataaa aaatacccga cagcagttaa
    23581 accagccatt gttttacctc tttagattgt attcatcaat aaagccagca aatttattca
    23641 tatgatttag aaataaattg cttaagcagt ttattaacct tctatcataa cgcgagcaaa
    23701 ccttgttcca caatgattgt ttgcttgaac agttcaagaa ttaatactaa cagaatgctc
    23761 ccaatatctt ccttaaaaag tatttttaat tcagtcgttt agtttatctt aattccctca
    23821 ttaaaaacat tttttgcctt gaccttgggg cttttgtaaa tcctgactga ctttaaaata
    23881 aaagaagtca acggaggact tcttgcgata gaatttttgt taactcaaaa gagttattca
    23941 acttttccta attgatgggc tttttgtttc gcagctttca tagcagaaaa aatgagctca
    24001 tccaaatctc catgcaggat ttccaaagct gcggctgttg tacctcccgg agaagttact
    24061 ttttttctca gttcagtcaa acttaactgg ctatgttgag ctaattgaag cgatcctgaa
    24121 aatgtctgta tggcaaagtg tttggcaacc gtttcttcca ggcccaattg gacccctgca
    24181 ttaatcatcg cttcaagaaa caaaaatacg taagcagggc cactgccaga aagggctgtc
    24241 agggtgtcca tgtcttcttc atctttaacc caatcagtaa tcccaatgct tgaaaataat
    24301 tgttctgccc attttttttg tttctcgttt acaaattcat ttgcaatcat tgatgttgct
    24361 gcaaatccaa ctatagcagg agtattaggc atggttctta cgactgcttg tcctttttta
    24421 caatatttgg caaaccaggt cagggacaat cctgctgcca ctgagattag aaggcaacct
    24481 gatgaaataa aagggttaat ctcattgact acctcacgca tttgtgcggg ttttactgcc
    24541 aatataatga cgtttgcgtc tttggccact tctttattat cgtaatgagt gcgaatattt
    24601 tctttactaa tgcccgcaga taaggatggt gaggcagctg acaatgagta tttattttgc
    24661 ttttgtaagt ggcaggcaat tgctttcgcc atattcccat aaccaataaa actgatattc
    24721 ataataattt accttttcgt tcgccaaaaa ttgctcgtcc aattcgaact atggtcgaac
    24781 ctgctttaat tgccggaacc aaatcatcac tcatacccat tgataaagta tccattttta
    24841 aatgaagtgc cttattgatg gattccttga gttgattgag tttaaggaat aaattgtact
    24901 gtgtttgcat gttgttttgc tgaggaggga ttgtcatcag tccccttagt tgcaaattag
    24961 gcaattgctt tacttcgaga gcaaggtcga tggcatgttc tggaggaatg cctgattttg
    25021 tagtttctcc agtcaaatta atttgtaaac agacattgag cggtggtagt tgctcagatc
    25081 tgaactggtt aagaagtttg gctgttttat ttcggtcaat gctgtgaacc caattaaaat
    25141 atttcgctat tcctttggtt ttattacttt ggataggccc tataaaatgc caacagatag
    25201 gcagatctct taatgccaat attttttcct gggcttcttg aaaatagttt tcaccaaagt
    25261 gtcttatccc ttgttggaat gcttctctga ttaattcggg gctttgttct ttactaacag
    25321 ccaataataa gacgctgttc ggttgcctgt tactggatga ttctgtctgg gttatcagtt
    25381 ctcttacttt gtttatgttc tgactaatgc tcatattaat cccggctaat caaaagatga
    25441 ggcgctttat tataaaaaga aatattaatt atgcatgaag tgtaatggta ctacaaaaag
    25501 atatccatga ttgctggtaa tcatttcatg agttaatcta caatatataa tgaaaaattg
    25561 gcgggaatta tagagtatgg atatcgcgga attattggca ttttcagtta aaaacaaatc
    25621 atcagatttg catatttcag ctggcatgcc tcctatgatt cgtgttgatg gtgatttacg
    25681 caaaattaat ttacctccat tagagcataa agacgtcatt aagattattt atgacataat
    25741 gaatgacagg caaagaaaag aatatgaaga attttttgaa acagattttt cctttgaaat
    25801 agccaatctg gcacggttca gggttaatgc tttcaatcaa tcgcgcggtg ccgctgctgt
    25861 tttcagaact attccttctg aaattcttag catggaagat ttaggattgc ccccaatttt
    25921 taaagaaatg gctagttttt ccaggggtct ggttctggtt actggcccaa ctggatcagg
    25981 aaaaagtacc acgcttgcag cagtcatcga ttatattaat tcaagtcgtt atgagcatat
    26041 tttgacagtt gaagatccca ttgagtttgt tcatcaaagc aaaaaatgtc tggtcaacca
    26101 acgcgaagta cacaaagaca cattgagctt taatgccgct ttgagatcag cgttacgtga
    26161 agatccggat attatattgg tgggtgaatt acgcgatttg gaaactatac gcttggcgat
    26221 gactgcagca gaaaccgggc atttggtttt tggaacattg cacaccaatt ctgcaacaaa
    26281 aacaataaac aggataattg atgtatttcc cgctgaagaa aaatcgatgg tacgctccat
    26341 gctttctgaa tcattgcaag ctgtcgttgc tcaaagttta ttaaaaaaga ataaaggtgg
    26401 tcgggttgcg gcgcttgaaa ttatgatgtg taccggggct attcgtaatt taattcgtga
    26461 ggataagata gcgcaaatgt attcgtcaat tcaaactggt caagccaaag gaatgcaaac
    26521 attggatcaa catttgactg aattggttaa taaaaatatt atttcgaaac acactgctca
    26581 tgaggctgcc ctgaacaagt cattatttta aatacacatg acttgtgaaa aagacccaag
    26641 gtcgagacaa aaaatgtttt taatgaggaa gtttatataa attaaatgac caaatttaaa
    26701 acattattta acgaagagat tagggccgca agtacagata gatttcaatg cggtgtttgt
    26761 atatacagga attgagcgca ggcaagttcc agaacccctc aaaaaacttt gaagatagca
    26821 atgccaataa ctgcaatgag atctcttcag ttagctcaaa gcattcctgc agagatcttt
    26881 tgttcaggtt tgaagtatta atttctatag cgggcgactg tttatgccgg gttattgttc
    26941 tcaggcattt ttttggattt caatcaattg aggaatacca atttcagcca atgacaacat
    27001 gctgttcagt tgttcccggt taaaggagtt atcttcagcg gtgccttgaa cttcaataaa
    27061 atgtccttgt tcattcataa ccacattcat atctgtttca gcaagaacat cttctgcata
    27121 atccaaatcc agcacgggtt gacccctgta aatacctaca gatacagcag ctacataatt
    27181 aaaagcaggc atcttcttta ttttttcacg ttgaaccatc cagtgaatcg catctcgcat
    27241 agccacacaa ctgccagtta tagctgctgt tctggttcct ccatccgctt gaataacatc
    27301 acaatcaaga gtaatagtat tttcgcccaa aacctttaaa tcgatacatg ctctcaatga
    27361 gcgccctatg agacgttgaa tttccaatgt tctgccacct tgcttacctt tactggcttc
    27421 cctttcggta cggctatgag ttgcacgtgg gagcatgcca tattcagcag ttatccaccc
    27481 ttggttttta ccttttaaaa accggggaac tccttcgacg attgatgcgt tacaaatcac
    27541 ttttgtctga ccaaattcga ctaatacaga gccttcggca taattggtaa aattacgtgt
    27601 tattgtaacg ggacgaagtt gatcatgttc acgattactg ggacgcatta gagatactcc
    27661 tctgtaaaat gcccaagtat aactctttaa tacttgagtt gcaccctaaa tacatacgct
    27721 atttgtacgt tcctctctat ttttgctgaa aaatattttt aatcaaacca ttcgatttgt
    27781 ttccattctt tacgttagaa acagtttgtt ttaagtttca agtttggttt aaggcctgac
    27841 tttcaggacc tgatatagaa gtttgttgca attaattgat gagcaatccc tgtgcaattc
    27901 ttttaatttc aggtatattc gcgcaaggtt aaaaagtagg tattctttat gattcatagt
    27961 atgacggctt tttctcgaat acaaaagcag tttgatgtag gtgatttttg ttgggaaatc
    28021 aggtctgtta accatcgcta tctcgatgtc tcttttcgtt tgccggagtc ctttcgattt
    28081 cttgaagcag aattaagaac tttgcttcgg gataagatac atcggggtaa actggagtgc
    28141 catctaaagt acaaggatac aagttgtgat aatcggtcta tgcttataaa tataggtacg
    28201 attaatgcat tacttgattt aagcagtaaa ttatctgctt ctcatcatct ggctaatgat
    28261 atgactgtca gtcaagtgct ttcatggcca ggcgttgtgg aggctaatct actggatatg
    28321 gaggaattgt ctcaaattgc tttggagtta tttcagaaaa gtatcgataa attgtgtgaa
    28381 gtcagagctg gggaaggcag gtcattaaag gcatatgttg aagatcgatt gcaggctttg
    28441 cgccaagaag taatgaaatc caaaagcatt gtggtttcaa tgagtgccaa gactaaagat
    28501 aaattactta cgcgtttgca tgaattgcaa atggacgttc cagaggcgcg tgtagagcaa
    28561 gaaattgcct tgctattagc caaacttgat gtgagtgaag agttggacag gttagagact
    28621 catatagcgg aagtaagccg cactttgaat gcagaaaagg tgacaggtag aagactggat
    28681 tttttaatgc aagaattaaa tagagaggcg aataccctaa gctcaaaatc ggattcagca
    28741 gagttaactt tatgtgctgt tgaaatgaaa gtattgattg agcagatgcg tgaacaaatt
    28801 cagaattttg agtaaatgat atggttagtg ataattcagg aaatttatac attgttgcag
    28861 caccctccgg aggagggaaa acaagtttgg tgaaaaaact cattgaaatg gtgggtgaga
    28921 ttgaagtgtc agtttctcat acgaccagac ctatgagacc tggagaaaaa gaaggggtgg
    28981 attatttttt cattgatgag gaacaattta tttctatggt gaatgagggg gcgtttattg
    29041 agcatgccaa agtatttaat cattggtatg gtacctcagt tgctcaaatt aacaaacggt
    29101 tacaatttgg aattgacgtc gttcttgata ttgattggca aggagcggag caaattagac
    29161 atgcctatcc ggatgcagtg agtgtattta tcattcctcc ttctttagac gcattaaaag
    29221 aacgcttgat gaacaggcga caagataaag atcatgtgat tagtgagaga atgactaaag
    29281 cacaggatga acttggccat tatcctgaat ttgattatct gattgttaat gatgattttg
    29341 aaaaggcggc aatggaatta cagtcgatag taattgccaa taggcttcgt attgaaaaac
    29401 aggtaaataa acaagccaaa ttgctttctt tcctcttgtc atcgcagtaa aataaaagat
    29461 ttgagttagt atttgttcta ataacctaga ggtggatttt atggcacgag taacagttga
    29521 agattgttta gaacatgtta aaaaccggtt tgagctggtt atggttgcta ccaaacgagc
    29581 aaggcaaatt gcagttcgtg gcgatcaacc catggttgaa tgggaaaatg ataagcctac
    29641 tgtagtagca cttagggaaa ttgccgaagg ttatgttaca gcggacattt tggatgaaga
    29701 ttaaaatgag ttattgactt tgccttggga atattagcca ttctaaaata tacctcttgg
    29761 taatcaagtt atggtattta agcaaagcaa agggaacaag ggcgaggcat aaaaccgtat
    29821 tttgagtaat gtgaggtata aatagccatg ttggatacta gggagtactg cgtgagctac
    29881 tttaaagagc tcgatgaaga gcttaaatgc taccttgagc aggcacaaat agagaaatgt
    29941 tatcaagctt atttggtggc tgaaaaagca caccatggcc aaatgcgtcg ttcaggtgag
    30001 ccttatatta cacaccctgt agcagcagcc ctcattctgg caagaatgag gctggactat
    30061 caaactatca tggctactct gcttcatgac gtcgttgaag atacctccat aagtaaagaa
    30121 gaactggtta atcaatttgg tgaagaagtc actgcccttg ttgatggtgt caccaagctc
    30181 accaaaatta aatttgaatc aaaggccgaa gctcaagcgg aaaattttcg caagatggtt
    30241 ttggctatgg ttaaggacat tcgcgttatt atcgttaaat tggctgatcg cttacataat
    30301 atgcgcactt taggatcaat gcctgcggcc aagcgcagac gaattgcaat tgaaacgctc
    30361 gagatttatg cccctattgc taatcgtttg ggtatgcatt ctatttatat cggccttgag
    30421 gatttgggtt ttcaagcttt atatcctatg cgttacaggg caattaagtc tgctgttgaa
    30481 aaatctcggg gaaatcgaag agagttgaca cagactatag agaaagattt gcaacacgcc
    30541 ttatcgcaat tgaacattcc ttatgaacaa gtatttggca ggcaaaagca tttatatagt
    30601 atttatagaa aaatgaaaca gaaaaaagca tcctttacag aaattacgga tgtgttcgca
    30661 tttcgcatca ttacagaaga cattgactcc tgttatagaa ttttgggagc tttacactgt
    30721 acttataaac ctgtcccaca acgatttaaa gattatattg gaattcccaa agccaatggc
    30781 tatcagtcat tgcataccac tttgtttggg ccttttggtg ttcctcttga ggttcaaata
    30841 cgcacaagag agatggataa agtagctgat aatggtgtag cagcgcactg gatctataaa
    30901 tcttccggat tggaagtcaa tgaagctcaa cttcgtgcca gagagtgggt tcaaagtttg
    30961 ctagaaatgc aaaggagcac gggcagttct cttgaattta tcgaaaacgt taaaatagat
    31021 ttatttcctg atgaagtata tgtttttact cccaaagggc acattatgga gttgccgaaa
    31081 ggagccactc ctgtagattt tgcatacacg gtacactctg gagtgggaaa tagttgtgtt
    31141 gcagcgaaag tgaacaggcg tttggttcct ttaagcattc ctttatccaa tggtcaaacc
    31201 gtagagatta ttacagcccc gggagcacac cccaacccgg cttggctgaa ttttgttgtg
    31261 actggaaaag caagatcaaa tattcgtcat tttctgaaaa gccaacaaca cgctgagtct
    31321 attgttttag gcaaacgatt attggagcaa tctttggctg agttatccag tgattacgcc
    31381 aaagtgcctc cggaaacatt acaggcattg ctcagcgatt tacactataa aacggttgat
    31441 gacttattgt atgccatagg aataggtaat cagatggcaa tggttattgc caagcggttg
    31501 gtggtttcgc aagacgccaa tgaatcggat aagggcaata aaaaaccttt ggctattaaa
    31561 ggcactgaag ggatggttgt gcattttggt gaatgctgcc aaccaatacc tggagataat
    31621 atagttggta aatttcaaca aggcagaggc attattgttc atgccagtga ctgcactaca
    31681 ctaaaaacaa ctcgtagtaa tccggaacag tttattgctt tgcgttggga tgagcaggtt
    31741 cagggtgaat attgggtaga cattactgta gatgtagcca acgaaagagg tgtattggct
    31801 gctttagcaa ctgctatttc agaagcagac tctaatatag ggaatattaa tgtggatcct
    31861 agggatggtc gtcataatgc ggtcactttt tccatcagtg ttcgtgacag aacccatttg
    31921 gctcgagtca tgcgtcgatt aagggcaaat aaagtagtta tgcggttata tcgaaaaaaa
    31981 caaggggagg tttaatgcaa ccaatcaata caaaattggc gccaaaagcg ataggcacgt
    32041 acagtcaagc gataaaaagt ggtgagatcg tttttctttc ggggcagatc ccattagatc
    32101 ctgaaacaat gcaaatctgc agcgatgata ttaaattaca aattactcaa gtcctggaaa
    32161 atttatcagc tgtatgtgaa gaggctggtg gtagtctggc taatattgtc aaattaaacg
    32221 tctatctcac tgatttaagt cattttccct taattaacga agcgatgagt cgatattttt
    32281 cagagcctta tcctgcaaga gcggcaatcg aagtctccgc attaccaaag ggtgccaagg
    32341 ttgaaatgga tgggattctg gttttgtctg catccgcata atcatgagtg atgatcaata
    32401 cgctcgcaca gggcgtctga tgttcttatt tgtttggatt atatttttta tagggttatt
    32461 tttattcttt tactattacg ataagccaga aagtaccatt tttgtagcta gtcgtaccga
    32521 gtatgtatta agcgctgata atgaaggaca ttattggata aaaggtaaga ttaatgaata
    32581 tcctgtagag tttttagtgg atactggcgc tactttggta gcaataccgc aaggcctggc
    32641 taaggattta aaaattaccg ggcgatatcc tgttaccata gaaactgcca atggcaaggt
    32701 aactggattt ttaactcgtt tgcaacattt gtcatttgga gagtttcact tacagaatgt
    32761 caaagcggtc ataatcccgg aaaatgatga taatacggtg ttattaggaa tgaatgtcct
    32821 atcacaattt catatgattc agcagggcaa acaattgata ttaaaaagac aatgaactga
    32881 ctgtgtgttt tcaataaaat gagatcaaac agatttattt aaatagagag ttttgatgag
    32941 tatagtttct cttaacggtg cctctttaat cttggctggt aattgtattc tggaccgtgc
    33001 agatttacag attcagccac aggatagaat tgctcttgtt ggacgcaatg gcgctggaaa
    33061 atcaacattg ctgaaaatac tacaagggga gttggtgctt gatagtggac aaattcaacg
    33121 ctcatccggt ttgcgtattt ctggtttagt gcaggaagtc ccaggtgctg agggagagtc
    33181 agtgtaccat tttcttgtta aaagcttagg tgaaacaggg gaagtattgt cccagtttca
    33241 tgagtttacg aaacaaggag atatggacaa attggccagg tgtcagcagc gaatggataa
    33301 cctgaatgca tggcatttat tacctgaaat tgaaacgatg gcaagccgat tgggaataga
    33361 tatccatgag cagatgtcta atctctctgg tggtatgaaa cgacgcgtat tattaggtgc
    33421 ggcactgctt gccaaacctg atttattgtt actggatgaa cctaccaacc acttggatgt
    33481 cgaagcaatt gaatggctag agaattatct aaaatccttt tctggtgctg taatggtagt
    33541 aactcacgac agggagttct taagccagat agcaacaagc atccttgaaa tcgatcgtgg
    33601 aaaattgtat ctacaccatt gcaattatga agcttacctt gacaggcgtg aatcgataag
    33661 gcttagcgaa caaaaacaaa atgatttgtt tgacaaacgg cttgcagaag aagaagcctg
    33721 gatacgtaca ggtatcaagg caagacgcac acgtaatgag ggtagagtca gggccttaaa
    33781 ggcaatgaga gagcagtaca aagcacgtcg tcatcagtta ggaacagtga aatccttaaa
    33841 tttggatgta agccgttctg gtgctcttgt agtagaagca aacaaagtga gttatgcaat
    33901 taatgacaag cccattataa aagatttttc tttattaatg acaagaggag acaagcttgg
    33961 gattatcggc cccaatggtt gtggcaaaac cactttagtt cgattacttc tgggagaaat
    34021 taagcctgat gctggaacta taaaattagg gacagggctt caggtagcct attttgatca
    34081 gttacgccga catttgcttg aagatcaaac agtcatgttt aatgtggggg aaggggctga
    34141 ttatgtaaca attaatggta agcaaaaaca tgttgccagt tatttgaaag agtttttatt
    34201 ttcctctgat cgatttaatc agccagtatc ttccttgtct ggaggggaaa gaaacagatt
    34261 attactggca aaactttttg ccaaaccggt taatttactc gtaatggatg aaccaactaa
    34321 tgatttggat atagaaactc tggaacttct tgaagctgta ttagctgatt atcctggtac
    34381 tttaatatta atcagtcatg atagagcgtt tattaatcaa gtggtaacca gtgtgttggt
    34441 ttatgaagaa aatggtaagt ttaatgaatt tgttggtggt tatgacgatt ataaaaaaca
    34501 tcaattaaca cgaaatgagc agcaaacgaa atctccagtt tcaaaacgga gcagcccaga
    34561 gaaagccaaa aatttatctt ttaatgaaca acgtgagtta tcgaagttgc cgcaacagat
    34621 tgaagccgta gagaaaaaaa ttgaagcact gcattcgcaa atggcctcac ctgaatttta
    34681 tcagcaagat gccaagctta tttcagaggt aactcagctg ttagccaagg aagaggcatt
    34741 gttaaatcaa tattttgctc gatgggaaga gct
//
LOCUS       DBNCWK010000020        34525 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.020, whole genome shotgun sequence.
ACCESSION   DBNCWK010000020 DBNCWK010000000
VERSION     DBNCWK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 34525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34525
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.020"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            159..1298
                     /locus_tag="AC06IO_000864"
     CDS             159..1298
                     /locus_tag="AC06IO_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HIO9874076.1"
                     /translation="MFRVLIFLSCLVILTPNTIADTNPCEKHSCIAVIDAGSTGSRLH
                     IYSYDTDDTNTPIHIEEIWNKKIKPGFASIQPNSVTIDAYLTMLLADAPIHNIPVYFY
                     ATAGMRLLPQSQQKKYYDELDYWFRQQSQWQLVEAKTITGNDEALFDWLAVNYKLDTL
                     KSVQNKSVGVMDMGGASVQIVFPMPKNAEISKHNQVELNIYGQNINLYVHSFLGLGQT
                     EMSHQFLNSPSCFANDYPLPDGESGQGNAPSCKEEVTSLMNSVHKVNQQIQPLLALNP
                     VNEWYSIGGISNLASSQLFHFENSELTNQSLLQQGDNQICHQQWDILNGQYPDDEYLY
                     QYCLLSSYYYALMVDGYGINPNQTIHYIPPEQNLDWTIGVVLHRA"
     gene            complement(1303..1836)
                     /locus_tag="AC06IO_000865"
     CDS             complement(1303..1836)
                     /locus_tag="AC06IO_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HIO9874077.1"
                     /translation="MHHVEDLISIFNYCFEKDYNTRLVRGGDEPIYLPADDKRPYHAL
                     FFAHGFFSSALHECAHWLIAGKERRKLVDFGYWYAPDGRNEEQQTLFQRVEVKPQALE
                     WILSMAAGHKFRVSIDNLNGTEVDTEAFKQSIYNQVFHYCTQGLPQRALHFRNALCAF
                     YRQPSPLRIDDFSIDEL"
     gene            complement(1847..3652)
                     /locus_tag="AC06IO_000866"
     CDS             complement(1847..3652)
                     /locus_tag="AC06IO_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1907 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874078.1"
                     /translation="MVRECTSFKGGLNLNFVENAEFESRKNIPPEEQRAVLIRNTLRL
                     LMMGWNTSWTELFSWETFKAIYLQRDPLLLKEFRLAFQKGFDALFTQLSGRQFNSQQN
                     EQIQLYLSNCLSYLPLFDLNPYESIKVPQRINDQWVMVDYYIKPIELTNHRGKNDLIL
                     RDDDRVFAYGLEPIDNPNAQSHLIFMGTTYPAGQGFISQLNTDLKGFETVGYSLYKNG
                     RDRIKDWILKQNSKIHVCGISLGGSLSLLLAIDMGKYLSRVDALNPAGLHDTDNKVKY
                     DKWYKIKKKPLVVVQQQGNDPVSFFGVWKKEWDILHVKPPKDKQGPNLFCDHFLNYAG
                     FAETEFTYVSAEQQNAKRKTRNFWLYSLGRGVLYYFGLAPYTYLIRPVIHYIAEQWPL
                     IVFGIIPLLTIGTLIGLSTAGIIPAIVSIGTLAAFAISSGIFYLSFGLSNNEEGKNTW
                     AGMFDFILKGNEKVTDENEIKLAKLHHPSTPRNPEMDIYKNETAIDLTYKQINTYYQI
                     TRCLLKQKEFLPLKDKPEKRFGGLSKKEALLASMNSASHDEVISLQIPKAKAVHIKHS
                     LTLAAKIGMENEIELKSALKKEYNQYQIGKHQRLC"
     gene            complement(3979..4584)
                     /gene="gstA"
                     /locus_tag="AC06IO_000867"
     CDS             complement(3979..4584)
                     /gene="gstA"
                     /locus_tag="AC06IO_000867"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HIO9874079.1"
                     /translation="MKLYFCRGACSLVVRIILNELGLNFQDEEVDLKVKKTAGGEDFF
                     KINPKGAVPTLVLDNGDVLTENQVILQYLADTTPGQKLLASVGESKRYHTLEWLNYIS
                     TELHKSLGMFFNPTVSEEIKSKILTPLVMTRFAYTNTRLAKGSYLMGNEFSLPDAYLF
                     VMVSWAKYFKLDLSQFEHLTKFMDLMLARPSVAKSLQQEKL"
     gene            complement(4781..5776)
                     /locus_tag="AC06IO_000868"
     CDS             complement(4781..5776)
                     /locus_tag="AC06IO_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874080.1"
                     /translation="MAICINKETDHFFISIGKINQHSFIMLGVYDDFQVSHLLCRVGK
                     IFDLPNQTKGIKRCLSIYSALGGAIFASSKAKIEDEGITRKRKGSAPISYQAYDISYE
                     QYCEFVHYLESIQTESNQFECFKPLVQNGNAVYFSQTSSRVFATGSHWKELNEEIHEI
                     NTGNTCRHSAIKLIEAVTKTSVPSSISSCFFINLPYKTQLDYGKPSQNIPFYVLPPPP
                     PSIHPGFNKEKCLIAKKLYHRIEQLPVLEPNSPMTKRKFNSLKNLYLQIIGSQKNQSI
                     DELLFGIQQWKEKNRVDLQTLRRTYFWDSFIFRESATMKLINEIEKDLKCAKCPY"
     gene            complement(5817..7079)
                     /locus_tag="AC06IO_000869"
     CDS             complement(5817..7079)
                     /locus_tag="AC06IO_000869"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIO9874081.1"
                     /translation="MKINNLVGFLLAFCLVSLPYNISAAESLVVSHHRIQKEMSKYFK
                     VYHNKEKFTAIAVSVFIPQEKNKDALDVATFVKGTMGYPPLSQEITPLNLFDIGSITK
                     SFTSLILLQLQTEGKLSLDDPLGKWLPQYKEWKDVKLRQLLNMTSGIPNYSEDPEFAK
                     KMEDNFAAIWGDEELLGYAHPEKPIETDRANRYEYSNSNYILAGMVIEKVTQDTFENQ
                     LKQRIINTNNGLNNSFYPAGPHGPEVEKSIRDRRVHGYFYDEDNKKLIDTISNDLSWA
                     GAAGAVIANTEDVGRWVQLLYHGLLIEPAFRDEALSELESVVSIKTGLPIPSVTEDEP
                     YGFGLGVGFYYDKNSRQRFWTYQGSTLGYRVMYLWNPCNNVTVVVALNGKGGSGNEKS
                     KMGNHIHELCLNIHKVIVKNHPHLQCAS"
     gene            complement(7327..8739)
                     /gene="gltX"
                     /locus_tag="AC06IO_000870"
     CDS             complement(7327..8739)
                     /gene="gltX"
                     /locus_tag="AC06IO_000870"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIO9874082.1"
                     /translation="MTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDT
                     DRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLEEGKAYRCQCSK
                     ERLEALREAQLAAKEKPRYDGHCRNQSLPDSGIPYVIRFRNPDGGIVSFHDEVYGDIH
                     VDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDA
                     PVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIF
                     SVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLN
                     QGPDLKDLVAVQAERCKSLAEMCQISQYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHE
                     RLKALDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKN
                     KSIKRLEDALEKIRARASAV"
     gene            8838..11570
                     /gene="letS"
                     /locus_tag="AC06IO_000871"
     CDS             8838..11570
                     /gene="letS"
                     /locus_tag="AC06IO_000871"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LetS"
                     /protein_id="HIO9874083.1"
                     /translation="MLKSIGIKYQLRITTLIPAFLVALLFAFFYNGLFGKDLKQHMSR
                     LGEAYIRQLLPAAQYAMLRNDYRTLQGLINASTINPEVKALAFYNADGRLIAYRGGKH
                     SIHKPFNPPDYTGDYIESKQINPFTINFIAPITIPKFNLYSSTEFKEPSTPKIFQADD
                     ILGWLSIDIDTQSLLIKRYQMLIVTIFITLFGLLMGLTIHYFLSKRIYMPIARLRRSM
                     KQILSNEFETEIRVSSPGELGIIEKGCAHLQRQYLNTVRDLNHHIEIATADLQQSLEL
                     LEEKNIELSLEKKKTEEKSRQKSEFIANMSHEIRTPMNGVIGFTNVLLESKLDPLQLD
                     YVKTIKSSAQDLLSIINDILDFSKIDAGKLNLDCIPLDVRGCIDEVLSLASPNAHKKG
                     IDLIPITDINVPKMVLGDPLRIKQIISNLVTNAVKFTDHGYVLIRTKIEQETDKDYTL
                     LFAITDTGIGISPEDQTKLFTAFNQADTSITRRYGGSGLGLVICKKLCEEMHGRISLT
                     SEINKGSTFSARIKVEKLVAYEIEKNQTHRFAHLKIICFDDNPLHLEAMSNGLGFWGI
                     EAIRVDSFNKLSRTLTKHKDCKIAFINVNQGCERQAAELIAKHKQIPFVLISKWPIND
                     FAALGARGFLYKPISIQKLQDLIESIANENQTEKNTNQELDTLREQLRFLHPEILIAE
                     DNPVNKMLLTSLLNNNANITTVDDGEMAVTACEDKKFDMILLDLHMPKLNGLEAAKMI
                     HQKSLMNKHSPIVLITASSSDLSSIDMKKSGVDFCFQKPIDEKQLLIQILRIVDKTKH
                     AAIDWQLCVQKVSGNQALAEEFLAKFIEELYKNREEFIGLMHQKNVKGLADLAHKLHG
                     ACCFCGVPILQKRVAQLEKLARRTANADNLSEAFTDLIQSIDAVISEYENQYSQ"
     gene            11667..12911
                     /locus_tag="AC06IO_000872"
     CDS             11667..12911
                     /locus_tag="AC06IO_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIO9874084.1"
                     /translation="MSVKNAIYAQSGGVTAVINASACGVIQTAKLFPEQIGKVYAAKN
                     GIIGALNEELIDTSLESEEDIAKLMQTPSGAFGSCRYKLKDNGEEYQRLIAVFKAHNI
                     GYFFYNGGGDSQDTAHKVSQLGKTMGYPITCIGIPKTVDNDLPFTDNCPGFGSVAKYI
                     AISTKEAGFDVASMAASSTKVFILEVMGRHAGWIAAASGLAEQNPGEPPHIILLPEVP
                     FEPTRFLKKVKDCVNSYGYCVIVVSEGIRNKEGKFLSEAGLRDAFGHAQLGGVAPVIA
                     QLIKSELGFKYHWAVADYLQRAARHIASQVDLDQAYALGKAAVEYAVKGYNAIMPIVI
                     REQNEPYQWSIGQVPLAEVANQEKSMPPEYIAEDGMGITDACKRYLSPLIQGEAYPNY
                     KNGLPDYVRLKNQLVAKKINQD"
     gene            complement(12908..13321)
                     /locus_tag="AC06IO_000873"
     CDS             complement(12908..13321)
                     /locus_tag="AC06IO_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIO9874085.1"
                     /translation="MRTKGFTLIELMIVAAILGILVAIAIPAYQDYTIRARVVEGLEM
                     TSAAKLAVVETSLIDNTLPANQADTGYTSPAPTINVASIAIGNQGIITITYTANAGNG
                     TITMVPTLDATGRILTWDCTGGTLISKYRPAYCRP"
     gene            complement(13518..13928)
                     /locus_tag="AC06IO_000874"
     CDS             complement(13518..13928)
                     /locus_tag="AC06IO_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIO9874086.1"
                     /translation="MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTL
                     ADSAKLAVSETAITNNALPATQAATGYVSPAATPNVQSIAIGANGVITITYTAAAGGG
                     TIIMTPTLQANGDVTWTCTAGTLLAKYRPASCRP"
     gene            14231..14548
                     /locus_tag="AC06IO_000875"
     CDS             14231..14548
                     /locus_tag="AC06IO_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIO9874087.1"
                     /translation="MTRKLRIEEKLISALSPLFLSVEDESMNHHVPQGAETHFKIIAV
                     SNHFNDLTRINRHKLVNQLLKEEFTLGLHALSMHLYTSDEWMTQQQSVLKSPACRDGY
                     KNK"
     gene            14623..16044
                     /locus_tag="AC06IO_000876"
     CDS             14623..16044
                     /locus_tag="AC06IO_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIO9874088.1"
                     /translation="MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIF
                     SAIIFLIPVALVAAELSSTWSDEEGGIYSWVKHAFGENIAFFAIWLQWINTLVWYPTI
                     LSFIAGTISYLINPELAQNKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMI
                     VPMGFIILLAFIWLIHGHPIAIDLSPSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVH
                     VRNVNNPQKNFPKAMFFSVILILFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQV
                     YDLNWLMPVLILLLLLGTLGSMINWIISPAKGLLMAAKHDFLPASLCHVNKHGMPAKI
                     LILQAILVTVLCSGFLLFPTVNAIYWLFTALSTELYMMMYVMMFIAAWHLKSKFSDME
                     RPFAIPGGKPGYYLTCLLGLLGCTVTLWVGFIPPTESINIGGTAHYRMVFSLGIVLML
                     LPAALIYLRKRHITKSKALNAPV"
     gene            complement(16174..17604)
                     /locus_tag="AC06IO_000877"
     CDS             complement(16174..17604)
                     /locus_tag="AC06IO_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIO9874089.1"
                     /translation="MKIFKFSSWLLLFVFTLDANAYYTSQGSIYDKSGNRVTINGVSW
                     SGFQDTNVFQGLQSNPFYNITMPQSKSRNGLMDLLVHPWSMADSGVNSSTAVEFKTVR
                     LPIQPGVLYDDSGEVDLNKWLSDKTQPETGNGLFCKTWQSNGQSCEKAVSPKQAFWTV
                     LAEMKKHNINVMIDFHHRYGYGDGMRDGTVYSMTQYEKDLVLLAQEIKQRGLDNVIGI
                     DVFNEPYQLSWFKARNGQVSWIKVIGTAAKAVHQVNPDLLLFVEGPGGGNDDMDNPTI
                     CMPKANIKEDSGYAHWRDPGQCGNDQEVVAFKGNWGEDFKPLLDKNLAKQNIAQFDVQ
                     KFSNELQLAVPGIDTETLGWLLGDDNMGNNGHLVFSPHVYPKEVAGWETAPGDASNLR
                     FDWSWGFLYKAGYPIVLGEASWKSFEGKNFFTQALMPYLTQSGIGSNNLYFWAIGYLG
                     DTVSAINPNTGELNLDVQQTLKPYFN"
     gene            complement(17852..18604)
                     /gene="kdsB"
                     /locus_tag="AC06IO_000878"
     CDS             complement(17852..18604)
                     /gene="kdsB"
                     /locus_tag="AC06IO_000878"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIO9874090.1"
                     /translation="MSHNFHVIIPARYHSSRFPGKLLQEINGITVIERVYRQALLAEP
                     MSVIIATDHDEIADRAIQFGAEVVITSHTHQTGTDRIAEVVAKGSFAPDDVIVNVQGD
                     EPFIRPQLIQQVACSLTKTKAPVSTLCWPISSLQILNNPNVVKVVRTRDNHALYFSRS
                     AIPFHRDDKNAYSNTFRHIGLYAYRAAFLLEFVSWPPCTLEQIECLEQLRILWSGFSI
                     RVEEACEEPLQDINTKEDLILAQRYFLDTFNV"
     gene            complement(18601..18780)
                     /locus_tag="AC06IO_000879"
     CDS             complement(18601..18780)
                     /locus_tag="AC06IO_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019231979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIO9874091.1"
                     /translation="MDKKLLEILVCPLCKGKLLFKKHELICKFDRLAFPVRDDIPVML
                     EQEARLIPLEEKDKL"
     gene            complement(18773..19444)
                     /gene="tsaB"
                     /locus_tag="AC06IO_000880"
     CDS             complement(18773..19444)
                     /gene="tsaB"
                     /locus_tag="AC06IO_000880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIO9874092.1"
                     /translation="MMKLLAIDTSTELASVATLIDDEIISREQDSQRIHAQLILPMID
                     ELVAQTGLGLNQLDGIIFGCGPGSFTGLRIACSIAKGLAYANDLPLVPVSSLAAIAWT
                     AREIKEDFNQPVLSVLDARMHEMYWSCFLEQQFLAQDRINAVKDIQLPANQSFILAGV
                     GIDLYWKDFPEQIKSQISEVLTVFPTASAMIRLAQKANIKAVSVAQAQPVYVRNQVTQ
                     GDSRG"
     gene            complement(19422..21380)
                     /locus_tag="AC06IO_000881"
     CDS             complement(19422..21380)
                     /locus_tag="AC06IO_000881"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIO9874093.1"
                     /translation="MEEITTFAETCRRTLSEKGRLSTAIPGFVARAPQADLAVAIANA
                     IVQRSILVAEAGTGTGKTFAYLLPCLLSGKKALISTATKTLQDQLFQKDLPTLIRALG
                     LSVRIQNLKGRSNYICQYRVEKHAEEGQFQSPQCAHEILHVRDKLSQMQEGVRSELPE
                     INEDSSVWHYVTSTTDNCLGAECPNHETCFLVKARKRAMDADIVVINHHLFFADSQLK
                     KEGFSELLPGVDVVVFDEAHQLADIASNFNGERLGTRQFKDLVADMLNEWPILDLANQ
                     PLKSISHKADKLVDQLLNALPTREERISWDLVKRNKEFMEAWDTWLSLKDELVQCLES
                     ENATDIPRLKRCKERLLDLETILLSFTEENNNKIRWLERFKHTLVFHATPYDVAESFS
                     QLLKHQSCAYIFTSATLTMASAFDSFCKPLGLDKVQTLLLSSPFDYQQQALLYLPRGI
                     PDPKDAHYYDALVEKALPVIEACGGRCFFLFTSHKAVKLVAQMMSNTLKYPLLIQGDE
                     AKPILLSRFRQLGNAVLLGTSTFWEGVDVKGEALSCVIIDKLPFANPVDPVIRGRMSY
                     LKKKGLSGFDELSLPNAVIALKQGVGRLIRDITDKGILMIADPRLTGREYGRTILASL
                     PGLPKTRDEEKVLHFIKELALNDETVSD"
     gene            complement(21368..21703)
                     /gene="fdxA"
                     /locus_tag="AC06IO_000882"
     CDS             complement(21368..21703)
                     /gene="fdxA"
                     /locus_tag="AC06IO_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIO9874094.1"
                     /translation="MTFVVTESCIRCKYTDCVEVCPVDCFYEGPNFLVIHPDECIDCA
                     LCEPECPVNAIVSEDDLTEEQQQFKELNAELSKTWPNITAKKDAPSDAKDWEEVKDKL
                     QYLQKEWKK"
     gene            21983..24778
                     /gene="legK7"
                     /locus_tag="AC06IO_000883"
     CDS             21983..24778
                     /gene="legK7"
                     /locus_tag="AC06IO_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector kinase LegK7"
                     /protein_id="HIO9874095.1"
                     /translation="MPLNLPPKSFKDITPLVIAYNNAPEDDKIQKLFYLQKINYLLNK
                     TQLNDDLFDWINDAEEGGWLNELAKFSINPNASFFLKGMQFAKAITEEIKNKPEINSS
                     EVNIYQLMQERDQLLKEVEFEKCATRYAEINFLLNELALNDKKTKEIVERQTEILRLV
                     APKIKAIKGESIDNLPVIPSYKTKELGNHVNNFNFKFTMSGWDAPFVFRVEDRHELGK
                     EQELHSYGVSKYFIEDYSVFMMRFKAEDGSTVYKPVILSQFANQNNLEEIAKQLKDGS
                     PKNIAPRIGYYFVQLTDFCLKLIETHNYHPDIKLNNFLVHNNRVLVSDRKTFTTSDNP
                     LASDILTSPLFAPDEFLKCLLFNKEGDPVGYNRNALWKRMNMPQFMAYQLGMALKQFL
                     ILTQLDELPDDFRNPDHSAVSHFKTPSRQIINLSLLVQELTRLDPDKRMTIKQFQTLL
                     NFKNLPPDAFYQKVEEVFPSSQLGIAEDIEALNKVLNSDLKGEALLKQANPVFTKLSK
                     YDPKETRLTRLAEKLAIRCFNNVSKPYFQKLSGLIESALEKDVNEIRQLLNPQSNSEG
                     LLQKASPIFMKLFERQPEVPQLTDLAKQLAAQCFDESSKNYFTRQLPLLVEEELLNQD
                     WEQAPWYRKALHWLTFGYFRVDNVTEISSLKIPEHAKGKEFQIYFSQLLFLPNEEIKN
                     MFTSKDSQTVDFLLGYIEKERERREMETPSVKIPVDLKGEEFQMHFPQLEFLPSKDFE
                     SIGEKEGEHLECFIFANLQEILSHNNSDSSEKNSTSEPTDSESEIDLNVGNAAGTIII
                     ADEPNPAETKPKKNDNDGIKQSNPAKTSPKGKVSTPEVDEHPKKKTKDDDTPKPKTKR
                     SVHFFDEAKKSKPKEKENASPTVDETPRAKRKNVCRIDSVRSTLFRGDGSHRQKFKEQ
                     RPRLSEIAWEPPKPNLQ"
     gene            25093..27738
                     /locus_tag="AC06IO_000884"
     CDS             25093..27738
                     /locus_tag="AC06IO_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874096.1"
                     /translation="MDVGQKIRILKRYLKEKSVTPEDVNLNDQDLTAYARLDKLLDYI
                     SIHNNGLFDFTTLESDEKQWKQYVDILNYSATCSPGSPPALIDTSERKAQFGLQYWLN
                     TRTIEALPRDNYYTRTVTLSEEADEEFDQLITESRAKLYEIAQTFPEISSFNPANIIQ
                     VTNQISSIKEKLDTLTSNNPQITLLQKKLNVLTDLNKINQLERLNIPQDNGSNLSYCT
                     LAETIEGVTEKIPLITLVKVKEQLRHLCAQNELDLDSYSESREQYYKLGIEGILKLPK
                     HGDIKEVQREAMALNISRLMGLDTTSSTTVSYNGHPALFIPFDSINLLNEFSSGKSFK
                     AGMGLSGQSYTHYSTIKPLGEGIQADLYIKDFGHALALLYLCCDTDAIGGYCKNKALR
                     NYSSLYIFDQVITDTDKFILDSRISLQPDQFIMKHTRHGQGRNRTLIEDSSLVTKYAS
                     LVRLKELKRIITQYCDHISWLHHNRTQAIKKQLQEELAKDERVRLTEELNDVEILEND
                     AEQIKSQILIRINKIAEVLPQTTGPVGSDVIRQCLILEKLLHNPVLYSDDGRPYKNPW
                     TTRQANTAQTINDLQNGKIQITFKFNIPEEMVDFIKRHGGGNSLTISSPKVITISKAH
                     LDGLRENMLHPEHSPELSLYTNYLDDKDLCIIKMAYRIGNRTKILNAIAEYRALMNNE
                     QVSFHNKIECLAQTEAKIKALIELANDEGLGMHILKKFYFDAQQRLQKLINPEVLPHD
                     IGQAFAAALRLDRVSEFNKVVREAIVQNKVTDPQFAIFLTSCIKIEFLAKNYKDAQKA
                     SRALLIESQRIINHLKSPVVPLIVQLGGQLHSRAELDTDGLANLENDLQLDFEKPDTE
                     SMNTMDLHQAVTKEEMIGDGMTVKV"
     gene            27814..28371
                     /locus_tag="AC06IO_000885"
     CDS             27814..28371
                     /locus_tag="AC06IO_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIO9874097.1"
                     /translation="MPVHERRQHFRIDDHIYFDYRILAPGEFCSDIEITNQLLGENGQ
                     KYMEAAQYFQGIDYELAELTQVMAVKEPALAHYLNLLNAKIDYLSRQMLMSSKVQLRK
                     VNISLGGMAFKTMEQIREKTNLKIILYTKPKMIPIIVDAVVVYSQYQSETHYRTAVAF
                     CNLTSEQEQLLSQHILQAQIKSRAD"
     gene            28447..28716
                     /locus_tag="AC06IO_000886"
     CDS             28447..28716
                     /locus_tag="AC06IO_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIO9874098.1"
                     /translation="MSRTVFCCKLKQEAEGLEKQPFPGELGKKVFNEVSKQAWNMWLS
                     HQTMLINEYRLNLIEARAREFLKEEMQKYFFGEGSDKPSGYKEIK"
     gene            28728..29654
                     /locus_tag="AC06IO_000887"
     CDS             28728..29654
                     /locus_tag="AC06IO_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIO9874099.1"
                     /translation="MFHDLLEFNEEVLDAINDKNPIVALESTIISHGMPYPDNLTTAI
                     EVENIIRRQGAIPATMAMHQGKIRIGLTQEVMEHLALQKEVIKASRRDISFVLSRKVT
                     ASTTVAATMFCAHMAKLPLFVTGGIGGVHQDVTMSFDISADLIELSNTPVTVVCSGAK
                     SILDLPKTLEVLETFGVPVIGYATDEFPAFYSRSSGIPVPQRLNSVEEVANLMSIQQK
                     LNMKNGIVVANPIPVSAELSDEEISPYIKQAHEEAKHMSGKSLTPFLLKRIAELTAGK
                     SLEANIELIKNNAFLGAEIAIAYQKKLFSKKT"
     gene            29705..29780
                     /locus_tag="AC06IO_000888"
     tRNA            29705..29780
                     /locus_tag="AC06IO_000888"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29738..29740,aa:Phe,seq:gaa)
     gene            complement(29822..29962)
                     /locus_tag="AC06IO_000889"
                     /pseudo
     CDS             complement(29822..29962)
                     /locus_tag="AC06IO_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30026..31177
                     /locus_tag="AC06IO_000890"
     CDS             30026..31177
                     /locus_tag="AC06IO_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HIO9874100.1"
                     /translation="MYPFILKPKSLRFFNLAFIALTLSFCLLANARAAGIERYDFRDM
                     TSKVTKAWDQDVALYVAKRFAVENSFDESEITVKKLNAGATNHFIYLMFINNELKFVI
                     KGLGTEKEAEALFALQNYSKIPDIQNNPQAATVTLSKIINSYRVKNDSRTYFFAILEA
                     AQGKELFKIMTYELLQHKDINTLKGVFYRIGQQMAELHKTIIGSDQFITPRDLVTVIH
                     DDFHPENTFYNLETNIFSLIDNESMGDSITEPLPIIRELYGFYEVPIIRWPSEKITVD
                     LLKDADPHDIASIYYDFVLGMASVYQDPQDAKQVLIEAIIDLNNMAIAFLKDRYSEPW
                     MLNPSDKHEAHIVWGKLAKVDTNPQLAEIYITKLTVINNDLQSLSGLKH"
     gene            complement(32078..32821)
                     /locus_tag="AC06IO_000891"
     CDS             complement(32078..32821)
                     /locus_tag="AC06IO_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874101.1"
                     /translation="MSRAKFFKSNRTHVIELYCYSNEYAQQVNHEITSGADSGPLLTK
                     IYGQDVRFIYAPDSEKFNLVLNEARKRNYNQPIINLYEPDNIKYLLSRLSHGDSILIN
                     GQGDIDKQLIAGRDAEELVDILENDLELKEISLKNLDIDSCMMGRVESYRHELKRHLK
                     NFQTITTYTDLCTASQSGGVPYRMWIEQRADRDVFYTESDLNKKGTRIIEYTDTYKNS
                     LKEIWKTNPYNLEEIDLSEYIDILVIASC"
     gene            33721..>34525
                     /locus_tag="AC06IO_000892"
     CDS             33721..>34525
                     /locus_tag="AC06IO_000892"
                     /inference="COORDINATES: protein motif:HMM:NF013752.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIO9874102.1"
                     /translation="MGKESSYKRRCSKLTPSAFIKALLSTSLCPYFNLEVFGSFLNKQ
                     KVSITKQGIHERFNKQTETFVKVLSTSFLNYFKTEKLPKLGQLEIFTSLNIIDSSSIS
                     LHSSLSQLFKGSGGAASGAALKIQLMFDYLIGQIKELTVTSGCDNDQGFDDYFHSIEA
                     GALYLMDLGYFKLNSFKKIREGHAFFVSRLLIGTKLFTLEKHPLDLLATLSTAESSFS
                     QQVLMGAKAQIPVRLITQKLPKEIADRRRQRLKEDHRRRGTKLSKEALAL"
BASE COUNT        11160 a         6881 c         6172 g        10312 t
ORIGIN      
        1 aaattgttaa tttttaatcc ataaaacagc gactattcat cattcgacaa accaatatcc
       61 cacaatgttt tgtacagttt atttaaacac actctattga caacaaaata tacacaatgt
      121 accattcgaa aacttcaatt ttggctctat atcaactaat gtttcgtgtt ttgatttttc
      181 tttcttgcct agtaattttg acgccaaaca ctattgctga cactaacccc tgtgaaaaac
      241 actcttgtat agctgtcatt gatgccggaa gtactggttc caggctacat atttactcct
      301 atgatacaga tgacaccaat actccaattc atattgagga aatttggaat aaaaaaatca
      361 aacctggatt cgcaagcata cagcctaatt ccgtaacaat tgatgcctac ctgacaatgc
      421 tattggctga tgcaccaatt cataatatac ctgtttactt ttacgcaaca gctggtatgc
      481 gactactacc ccaatcacaa caaaaaaaat actatgatga attggactac tggtttagac
      541 aacaatcgca atggcagttg gtcgaggcaa aaactattac tgggaacgac gaagcattgt
      601 ttgactggct tgctgtaaat tataaacttg atacattaaa atcagtccaa aataaatcag
      661 ttggtgtcat ggatatgggc ggcgcttccg ttcaaattgt ctttccaatg cctaaaaatg
      721 cggagattag caaacataac caggtagaac tcaatatcta tggacagaat atcaatctgt
      781 atgttcacag tttcctgggg ttaggccaaa cagaaatgtc acatcaattc cttaactccc
      841 cctcttgctt tgcaaacgac tatcctttgc cagacgggga gtcaggacag ggcaatgctc
      901 catcgtgcaa ggaagaagta acctcattaa tgaatagtgt ccataaggta aatcaacaaa
      961 tacaaccttt gcttgcttta aatcccgtga atgaatggta cagtattggt ggtatttcca
     1021 atcttgccag cagccaatta tttcattttg aaaacagcga acttactaat caaagtttgc
     1081 tgcaacaagg ggacaaccaa atttgccatc agcaatggga tatacttaat ggccagtatc
     1141 ctgacgatga gtacctctac caatattgtc ttttatcctc ttattactac gcattaatgg
     1201 ttgatggtta tggcattaac cccaatcaaa ccatccatta tattccgcca gaacaaaatc
     1261 tcgattggac aattggagtc gtgcttcacc gcgcttgaag cattacaatt catcaattga
     1321 aaaatcatca attctcaggg gagaaggttg acggtaaaaa gcacataaag cattacgaaa
     1381 atgtagtgca cgctgtggta acccctgggt acaataatga aacacctgat tatagattga
     1441 ctgtttaaag gcctcagtgt ctacttcagt cccatttaaa ttatcaatac ttaccctaaa
     1501 tttatgtccc gcggccatag aaagaatcca ctctaaagcc tgtggcttca cttccaccct
     1561 ctgaaacaga gtttgttgtt cctcattgcg tccatcagga gcataccaat agccaaaatc
     1621 gaccagcttt cttctttctt ttccggcaat aagccaatga gcgcattcat gcaaagcact
     1681 gctaaaaaat ccatgtgcga aaaatagcgc atgataaggc ctcttatcat ctgcaggaag
     1741 ataaataggc tcatctcccc ctctaaccag acgggtattg tagtccttct caaagcaata
     1801 attaaaaata ctaattagat cttctacgtg atgcacgaat cgttacctag caaagccttt
     1861 gatgcttacc tatctgatac tgattatact cttttttcaa agcagatttt aattctattt
     1921 cattttccat acctattttg gctgccaaag ttaaagaatg cttgatgtgc acagcctttg
     1981 ccttgggtat ctgaagagaa atgacttcgt catgcgaggc agaattcatg cttgctaata
     2041 aggcttcttt tttagataac cctccaaaac gtttctcggg tttatcctta agaggaagaa
     2101 actctttttg tttcagaaga caacgagtta tctgataata ggtattaatt tgcttataag
     2161 ttaaatcgat cgcagtttcg ttcttataga tgtccatttc aggatttctg ggtgtagatg
     2221 gatgatgaag tttggccagt tttatctcat tctcatccgt cactttttcg ttacctttta
     2281 aaataaaatc aaacatccct gcccaagtgt ttttgccttc ttcattatta cttaagccaa
     2341 aagacaaata aaaaattcca ctggatattg caaaagcagc aagagtacct attgaaacaa
     2401 tagcgggaat aattcctgcg gtacttaacc ctataagagt accaatagtc agtaaaggaa
     2461 ttataccgaa tacgattaac ggccattgct ctgcaatata gtgtattaca ggtctgatta
     2521 agtaggtata gggagccagg ccaaaataat atagaacccc tctccctaaa gaataaagcc
     2581 agaaattcct tgttttcctt ttggcatttt gttgctcagc agagacataa gtaaattctg
     2641 tttcagcaaa tccggcataa ttcagaaaat gatcacaaaa aagattagga ccttgtttat
     2701 cttttggagg cttgacatgt aaaatgtccc actctttttt ccaaacccca aaaaaagaca
     2761 caggatcgtt accttgctgt tggacaacaa ccaaaggttt ctttttgatt ttataccatt
     2821 tatcgtactt cactttatta tcagtatcat gaagtcctgc ggggtttaag gcatccactc
     2881 tggaaaggta ttttcccata tcaatagcca aaagcagact taaagagcct ccaaggctta
     2941 taccacatac atgaatttta ctgttttgtt ttaaaatcca gtccttaatt ctatctcttc
     3001 catttttata taaagaatag ccaaccgttt caaatccttt caaatcagta tttaattggg
     3061 aaataaaacc ttgaccagca ggataagtag ttcccataaa aattaaatga gactgggcat
     3121 ttgggttatc tattggctca agtccataag caaaaaccct atcatcatct cgcaaaatca
     3181 gatcattttt tccacgatga tttgtaagtt ctatgggctt gatgtaatag tcaaccataa
     3241 cccattgatc attgattctc tggggaactt taatcgactc gtaaggattt aaatcaaata
     3301 aaggcaaata actaagacag ttactaaggt aaagttgtat ttgttcattt tgttgcgaat
     3361 taaattgcct gccacttaat tgggtaaata gtgcatcaaa ccctttctga aaggctaatc
     3421 gaaactcttt cagcaacaga ggatctcttt gaaggtaaat agccttaaag gtttcccagg
     3481 aaaataattc tgtccatgag gtattccaac ccatcattaa caatcgcaaa gtattacgaa
     3541 tcagaactgc cctttgctct tcaggaggta tgttttttct agattcgaac tcggcattct
     3601 cgacaaaatt taaattcaaa ccacctttaa aacttgtaca ctccctaacc ataaaaaacc
     3661 tctgaatagc ttgtttataa ctatgagttc aagataatta caatcactgg ctcgcagact
     3721 gctaagctag tcatttaaaa accaagtaac tgtatattac tatcaattaa aagttggaat
     3781 tttagcacaa taacaaagat tattgctaat aacttaaccc caaagggttt attttgctca
     3841 aaaagatggg ctgcctaaca agaaagttaa acccccatac ttcaaagcat tggatataat
     3901 gactcttact cctcctttta aaaaatacta tatagaagaa gcaaattggg ttgcaatgaa
     3961 ttaataacac aacccgagct ataatttttc ttgctgtagt gacttggcaa ctgaagggcg
     4021 agccagcata agatccataa attttgtcaa atgctcaaac tgagacaaat ccagtttgaa
     4081 atattttgcc cagcttacca taacaaacag ataggcatca ggtaaactaa attcatttcc
     4141 catcaaataa gatccttttg ccaaacgagt attggtataa gcaaaacggg tcatgactaa
     4201 aggtgtcaaa attttggatt ttatttcctc tgaaacagta gggttaaaaa acataccaag
     4261 cgacttatga agctctgtag aaatataatt taaccattcc agagtatgat atcttttact
     4321 ctctccaaca gaagccaaca atttctgtcc tggagtagta tctgccagat actgtagaat
     4381 aacctgattt tcagtcagga catcgccatt atcaagaacc agggttggga ccgctccttt
     4441 aggattaatt ttaaaaaaat cctcgccacc tgctgttttt ttgactttta aatcgacctc
     4501 ttcatcctgg aagtttaacc ctaactcatt gagtataata cgaactacta atgaacaagc
     4561 acctctgcaa aaatataatt tcatattcac tcctttgatg atgtttaaat aataatagct
     4621 aagttagcat aaaatgcagc aaaaaacttg aaatgtaaaa aaccatacag tgaaaaagga
     4681 tgatcttgca tgtggtttat ttaatcagac cacgtttaat caggctctat aagaagcaaa
     4741 gattcgatat gctctaaccc tgatattatc ataacaatat ttaatatggg cacttggcgc
     4801 atttgagatc tttctcaatt tcattgatta acttcatagt agctgattcc ctaaaaataa
     4861 aagaatccca aaaataagtt cttcttaatg tttgcaaatc aactcgattt ttctccttcc
     4921 attgttgtat accaaacagc aattcatcaa tagattggtt tttctgtgaa ccaatgattt
     4981 gcaaatataa attttttaag ctgttgaatt ttcgcttggt cattggcgaa ttaggctcaa
     5041 gcacaggcaa ctgctctatt ctatgataga gttttttggc tattaagcat ttttctttat
     5101 taaatcctgg atggatagaa ggtggaggtg ggggtaaaac ataaaaggga atattttgac
     5161 tcggtttgcc atagtccaat tgagttttat acggcaggtt tataaagaaa caagaggata
     5221 tcgatgaggg gacagatgtt tttgtaaccg cctcaattaa ttttattgct gaatgtcgac
     5281 aagtattccc tgtattgatt tcatgaattt cctcattaag ctccttccaa tgggaaccag
     5341 ttgcgaaaac acgagagctg gtttgcgaaa aatagactgc atttccattt tgaacaagcg
     5401 gtttaaaaca ttcaaattga ttgctctcag tttgaataga ctcaagataa tgaacaaatt
     5461 cacaatactg ctcataacta atatcatagg cttgataaga aataggtgca gatccttttc
     5521 gttttcttgt aattccttca tcctctatct ttgctttgct actcgcaaaa attgccccac
     5581 ctaaagcact ataaatgcta agacaccttt taataccctt ggtttgatta ggcagatcaa
     5641 agattttccc tactctgcac agtagatgag atacctgaaa gtcatcatac acacctagca
     5701 taataaatga gtgctgattg attttaccta tcgaaataaa aaagtgatct gtttctttat
     5761 taatacagat tgccatgtga ttcagtcctt aacaaccaat aatgataaat aataaattag
     5821 cttgcacatt gtaggtgtgg atggttttta acgataactt tatgtatatt caaacaaagc
     5881 tcatggatat ggttgcccat ttttgatttc tcattgcctg agccaccttt cccattcaaa
     5941 gccacaacaa ccgttacatt attacaagga ttccacaaat acatcaccct gtaacctaaa
     6001 gtactgccct ggtaagtcca aaagcgctgc ctggaatttt tatcataata aaaacccact
     6061 cccaatccaa aaccgtaggg ctcatcttct gtgactgaag ggataggtaa ccctgttttt
     6121 attgaaacca ctgactccaa ttcagataaa gcctcgtctc taaaagcagg ttctatcagt
     6181 aacccatggt atagcagctg cacccaacga ccgacatctt cagtatttgc gataacagca
     6241 cctgctgcgc cagcccagga taaatcattg cttatagtat caattagttt tttgttatcc
     6301 tcatcgtaaa aataaccatg gactcgtcta tcccggattg atttttcgac ttctggacca
     6361 tgtggtcctg caggataaaa tgaattgttt aatccattgt ttgtattaat aatcctttgt
     6421 tttaactgat tttcgaaagt atcttgagtc actttttcaa taaccattcc agcaagaata
     6481 taattagaat tggaatactc atacctgttt gctctgtcgg tttctattgg cttttctgga
     6541 tgagcataac ctaacaactc ctcatcgccc cagattgccg caaaattatc ctccatcttt
     6601 tttgcaaatt caggatcttc ggaataattg ggaatgccac tggtcatatt caataattgt
     6661 cttaatttta catctttcca ctctttgtat tgaggcagcc atttacccaa agggtcatcc
     6721 agtgacaatt tgccctctgt ctgcaattga agcaaaatca atgaagtgaa tgatttggta
     6781 atgcttccaa tatcaaacaa attcaatggg gtaatttcct gagacagtgg aggatatccc
     6841 atagttcctt tgacaaaagt ggcaacatcc aatgcatcct tatttttctc ttgaggaata
     6901 aacactgaga cagcaatggc agtaaatttt tctttgttat gataaacctt gaagtacttg
     6961 ctcatttcct tttgaattct atgatgagat acaacaaggg attcagctgc gctgatatta
     7021 taaggtaaag aaaccaggca gaaggcaagc agaaaaccaa ctaagttatt tattttcatg
     7081 gcgaaaaata catcaagtta agtagaaaat tggccatttt aacctgatag actcaaatag
     7141 atccataaaa taaaaaaaaa caggaaatgt tctgttgaat atacggacac gcctattcgc
     7201 attctcttcg gccaggagca atgaaatcca gtgaaattgt agttttcatt gcccacttga
     7261 aatatgaatg cttctccaaa tacaaatctt tttgcattca gtttaaaatc aataaatatt
     7321 cagattctaa acagcactgg ctctggctcg tattttctcc aaagcgtctt caagacgttt
     7381 gattgattta tttttgccta acaaagccaa tgtcatgtca atggaaggag atgtaccgga
     7441 gccggtcacg gcgacacgca acggttgagc gattttacct aaattcaaat caaattgcag
     7501 gctcacatca ttaatgcatt cttgaatttt atcatttttc cattcgtcaa gcgcttttag
     7561 gcgctcatgc aataccatta aaggctccag aacaactggc cgcaagtgtt ttttaacagc
     7621 atcttcatta tattcaatgg tatctgtata aaaatactga ctaatttgac acatttctgc
     7681 taatgactta catctctctg cctgaactgc gaccaaatcc ttcaaatcgg gaccttggtt
     7741 caaatcaatt cctgcctgct caaaatgcca ttgcaacgca ttggcaactg attctggaga
     7801 gtcacttttt tgataatgct gattcaacca atacaactta tcataattga aacttgagac
     7861 acctcgactg acatttttca agtcaaagct ggttatcatt tcttgcacac taaaaatttc
     7921 ctgatcgcca tgagaccatc ctaaacgaac taaataattc aataaagcat gtggcaatac
     7981 acccagctcc ttgaactgca agacacttac tgcaccatgt cgctttgaca gccgtttgcc
     8041 atcttcaccc aaaatcatgg gcaaatgggc aaaaacaggt accggcgcat ccaatgcctt
     8101 gaataaatta atttgtctcg gagtattgtt gatatgatca tccccgcgaa taacatgagt
     8161 tattttcata tcccaatcat caataactac agcaaaatta taggtggggt gcccatcaga
     8221 acgaaccaaa atcaaatcat ccagttcgct attatccaca tgaatgtcac cataaacctc
     8281 atcgtggaaa gacactatac ctccatcagg atttctaaag cgaataacat aaggaattcc
     8341 actatcagga agactttggt ttctacaatg tccgtcatag cgtggttttt ccttggcggc
     8401 taattgagcc tctcttaaag cttccagcct ctccttactg cattgacagc gataagcctt
     8461 tccttcttcc aaaagttgct gcgctacttg ttgataacga gcatatctct cagtctgata
     8521 ataaggacct tcatcgaagt taagccctaa ccaggccatt ccgtctaaaa tagcctgtac
     8581 agattcttga gttgaacgct ctctgtccgt atcttctata cgcagtataa attgcccgtt
     8641 gtgatgtttt gcatatagcc atgaaaataa agcagtcctt accccgccaa cgtgtaaaaa
     8701 accagtaggg cttggggcaa atcgggttct aacagtcata atatcttcca ttgtaaggaa
     8761 taaaataatc aagttataat agccgagttt accatgaacg caaagattca actgctgaat
     8821 taagactaaa aggagctatg ttgaaaagta ttggtattaa atatcagcta agaatcacta
     8881 ctcttattcc tgccttttta gtcgccttgc tttttgcttt tttttataac ggcctatttg
     8941 gtaaagatct aaagcagcat atgtccagac tgggtgaagc ctatattagg caattacttc
     9001 cagcagccca gtatgccatg cttcgcaatg attaccgtac attgcagggt ttaatcaatg
     9061 cctcaaccat taacccggag gtaaaagctt tggcatttta taatgccgat ggccgattaa
     9121 ttgcttatcg tggcggcaag cactctatcc ataaaccatt caatccacct gactatacag
     9181 gtgactacat tgaaagtaag caaattaatc cattcaccat taattttata gcgccgatta
     9241 ctatcccaaa gttcaatttg tactcaagca cagaattcaa agagccttct acgcctaaaa
     9301 tattccaggc tgacgatata ttaggctggt tatccattga cattgataca caatcactac
     9361 ttataaaacg ctatcaaatg ctcatcgtta ccatttttat tactctattc ggtttactga
     9421 tgggtttaac aattcattac ttcttgtcta aacgaattta catgcctatt gctcgattgc
     9481 gtcgaagtat gaagcaaatt ttaagcaatg aattcgaaac tgaaattcgt gtttcaagtc
     9541 caggagaatt aggaataatt gaaaaaggtt gcgctcattt acaacgacaa tatttaaata
     9601 cagttcgtga tttgaatcat cacattgaaa tcgccacagc agatctgcaa caaagtctcg
     9661 aacttctgga agaaaaaaac attgagctat cactggaaaa gaaaaaaact gaagaaaaaa
     9721 gtcgacaaaa atcagaattt attgccaaca tgagtcatga aattcgcacc ccaatgaatg
     9781 gcgtgattgg ttttacaaat gttttattgg aaagcaagct ggacccttta caactggact
     9841 acgttaaaac gatcaaatca tcagcgcagg atttgttaag tatcatcaat gatattctgg
     9901 atttttctaa aattgatgcg ggcaaattaa atcttgattg catcccgcta gacgtaagag
     9961 gttgcattga tgaggtttta tccctggcaa gccccaatgc gcacaaaaaa ggaatcgatt
    10021 taatcccgat tacagacatc aatgtgccca aaatggtatt gggtgaccct ttaagaatca
    10081 aacaaatcat cagtaatctg gtgaccaatg cagtaaaatt tacagatcat ggatacgttc
    10141 ttatacgcac taaaatcgag caagaaacag ataaagatta taccttacta tttgctatta
    10201 cggatacggg aataggcatt tcaccagagg atcaaacgaa actgtttact gccttcaatc
    10261 aggcagacac cagcatcaca cgccgttacg gtggctcagg attaggcttg gttatatgta
    10321 aaaaactatg tgaagaaatg catggacgta ttagtctaac cagtgaaatc aacaaaggct
    10381 ctacttttag cgcacgcatt aaagttgaaa aacttgtggc ttatgagatt gaaaaaaatc
    10441 aaactcatcg tttcgcccat ttaaaaatca tttgctttga tgacaatccc ctccatttgg
    10501 aagcaatgag taatggcctg ggattttggg gtatagaggc tatacgcgtt gactcattta
    10561 ataaactttc ccgaacattg acaaaacata aagattgcaa aattgccttt attaacgtca
    10621 accaaggatg tgagcgacaa gctgctgaac tcattgccaa acacaagcaa attcctttcg
    10681 tgttaatttc taaatggcct attaatgatt ttgcggcgct tggtgctcgg ggttttcttt
    10741 acaaacctat cagcatccaa aagcttcagg atctgattga atcgatagcc aatgaaaatc
    10801 aaactgagaa aaatacgaac caggaactgg atactttacg ggaacagctt cgttttcttc
    10861 atccagagat actgattgca gaagacaacc ctgtcaataa aatgctatta acatccctat
    10921 taaacaataa tgccaatata accacggttg acgatggcga aatggccgtt actgcttgtg
    10981 aggataaaaa atttgacatg attttactgg atctgcatat gcccaaactt aatggtttgg
    11041 aagcagccaa aatgatacat caaaaatccc taatgaataa acactctcct atcgttctca
    11101 ttaccgccag tagcagtgat ctgagctcca tcgatatgaa aaaatctgga gttgattttt
    11161 gttttcaaaa acctatagat gaaaagcaat tgttaatcca aattcttcgc atcgttgata
    11221 aaaccaaaca tgctgccatt gattggcaat tatgcgttca gaaagtttca ggtaatcaag
    11281 cgcttgctga agagttcctc gccaaattta tagaagaatt atataagaat cgtgaagagt
    11341 ttattgggtt aatgcatcaa aaaaacgtaa agggcttggc tgatttagct cataaattac
    11401 atggcgcctg ttgtttttgt ggcgtgccta ttttacaaaa gagagtagct caattagaga
    11461 agcttgcaag acgaacagct aatgctgata atttaagcga agcctttacc gatttaatac
    11521 aaagtattga tgcggtgatt agtgaatatg aaaatcagta ctcacaataa catgggatga
    11581 aataaggcca taaaaactct cttgtatgaa aaccaaagta aaagtctgta tcaacctaat
    11641 aaaatcattc tagctatgga gacaaaatgt cagtaaaaaa tgcaatctac gcccagtcgg
    11701 gtggcgtgac cgctgtaatt aatgcatccg cttgtggtgt catccaaacc gccaaactat
    11761 ttccagagca aattggtaaa gtttatgctg caaaaaatgg cattatcggt gccttaaacg
    11821 aggaattaat cgatacttca ttggaaagtg aagaagacat tgccaaattg atgcagactc
    11881 cttccggagc ttttggctcc tgccgctaca aattaaaaga caatggggaa gagtatcaac
    11941 gcttaatcgc tgtcttcaag gcccacaaca taggttattt cttttacaat ggcggcgggg
    12001 actcacaaga tactgcccat aaagtatccc agttaggtaa aaccatgggc tatcctatta
    12061 cctgcatagg tatccctaaa acagtagata acgatctgcc ttttacagac aattgcccgg
    12121 gatttggatc agtcgccaaa tacattgcca tttcaacgaa ggaagctggt tttgatgtcg
    12181 cgtccatggc agcttcatcc acgaaagtgt ttattcttga ggtgatggga aggcatgcag
    12241 gctggatagc tgcggctagt gggctggccg agcaaaatcc aggcgaaccg ccacacatta
    12301 tcctattgcc tgaagttcct tttgagccaa ctcgttttct aaaaaaagtg aaagactgtg
    12361 tcaattcata tggttattgt gtgattgttg tttcagaagg tatccgcaat aaggaaggaa
    12421 aatttctcag tgaagcagga ttacgcgatg cattcggaca tgcccaatta ggtggtgttg
    12481 caccagtcat cgcccaattg attaaatcag aacttggttt taaataccat tgggcagttg
    12541 cagattatct tcaacgagca gccagacaca tagcctcgca ggttgatttg gatcaagctt
    12601 atgctttagg taaggctgct gtggaatatg ccgtaaaagg atataacgcg ataatgccta
    12661 ttgttattcg tgaacaaaac gagccttatc aatggtccat aggtcaagtc cctctagctg
    12721 aggtagctaa ccaggaaaag tctatgccac cagagtatat agctgaagat ggcatgggaa
    12781 ttacggacgc atgcaaacgt tacttgtccc ctttgattca aggtgaagct tatcccaatt
    12841 acaaaaatgg attaccggat tatgtccgac taaaaaacca gttagtggct aaaaaaatca
    12901 atcaagatta agggcggcag taggctggac gatatttact aatcagggta ccaccagtgc
    12961 aatcccaagt gagtattctg cctgtagcgt ctaatgtagg aaccattgta atagtaccat
    13021 tacctgcgtt tgctgtgtag gtaatggtaa taatgccttg attaccaatg gctatagagg
    13081 ctacattaat tgttggggca ggactggtat aacccgtatc tgcctgattt gctggcaaag
    13141 tattatcaat taaactcgtt tcaacaacag ctaatttagc ggccgaagtc atttccaagc
    13201 cttcgaccac tcttgctctg atggtatagt cctgataggc tggaattgcg atggcaacca
    13261 gaatcccaag gatagcggca acgatcatca gctcgattaa agtaaatcct ttcgttctca
    13321 taacgtcacc tggcattgca tctaactgat atcacgcaaa ttcaatgcca actctatgat
    13381 ttaaaaatcc ttctgctgac taaatagctt gacaagcttc tttcaaaaca tttctcttag
    13441 gtaaataact cattggtaaa acctgggcaa ataaaaaacc gccattgaaa ggcggttttt
    13501 atagttgctt tatccgatta tggtctgcaa ctggcaggtc ggtatttggc caataatgta
    13561 ccggccgtac aagtccaggt tacgtcaccg ttggcttgca aagttggggt cataataatg
    13621 gtgccgccac ctgctgcagc agtatacgtt atggtaatca caccgttcgc accaatagca
    13681 attgattgta cattgggcgt agcagcggga gacacgtagc ctgttgcagc ctgagtagca
    13741 ggtaacgcgt tgttggtaat agccgtttca gatacggcaa gttttgcaga atctgccaag
    13801 gtcagacctt cagtaacacg agctcgtatt gtgtaatctt gataggctgg tatagcgatt
    13861 gctgccaaaa taccgataat cgctacgact atcatcaatt caattaaagt aaaacccttt
    13921 tgtctcatga ccatctccat agttaactat ccattagtat aatgcaagca taatgccaac
    13981 taccagccaa tattctaaac ctgtattatt ttacatgtta aataatcatt tcatgtaaaa
    14041 aacaccgttt tttgtgctaa aaattcagtg aaatgaatag aaaaaatatt tcataaaata
    14101 aactatttgt tattatataa caataaaatg acaatttttg tcacattatg acaattgtgt
    14161 ttaattaatg tgacaactat gcttcaaatg tatatactcc ttattttgca aaaacagagt
    14221 cgagcaaatc atgacgcgta aactccgcat agaagaaaaa ctaatctccg cattatcccc
    14281 cctctttttg agtgttgaag acgagtccat gaatcatcac gttcctcaag gcgctgaaac
    14341 acattttaaa ataatcgccg tttcaaatca ttttaatgat ctgactcgta tcaatcgtca
    14401 taaattggtt aatcagcttc ttaaagaaga atttactctg gggctgcatg cattaagcat
    14461 gcatttatat acttctgatg aatggatgac tcaacagcaa tcggtactca aatcccctgc
    14521 ttgcagagat ggctataaaa acaaataatt ttaatgaaaa aagttcacct ccaagcttga
    14581 tttattttat aatgtctatc tttttatcaa atagaaaaat caatgaaatc atggtcctct
    14641 gaaaaaatat ccgttcttgc tttagtgctg ctcattaccg gagcaattga cagcattaga
    14701 aatttgccag gcacagcact ttttggatct actttgattt ttttctttat tttttcagca
    14761 attatctttt tgatcccggt tgctttagtc gctgctgaac tgtcctcaac ctggtcagac
    14821 gaggaaggtg gcatttacag ttgggtcaag catgcctttg gtgaaaacat tgcctttttc
    14881 gccatttggc tgcaatggat aaatactctg gtatggtatc cgactatttt gtcttttata
    14941 gcaggcacaa tctcttattt aattaatcca gaacttgcac aaaacaaata ctatcttatc
    15001 agcgttattc tgattatttt ctggtcatta accttgatag gcttatcagg gctgcgtgct
    15061 tctgccacct ttgctggctt ctgtgcaata gttggaatga tagtgcctat gggatttatc
    15121 atccttctgg cttttatttg gttaattcat ggccacccta ttgccattga tcttagcccc
    15181 tccaatttgc taccgcaatg gaaaaacaca caatcctggg tttctctaac agcgattatg
    15241 acttcttttc tcgggatgga gcttgccgca gttcacgtgc gcaatgtcaa taacccgcag
    15301 aagaattttc ccaaagcgat gtttttttcg gtcattctca tcctattcac catgatttta
    15361 ggttcactgg ccattgcttt cattctgcct aaagacaaaa tcagtttggt agatggggtt
    15421 atgagggcat ttagtaattt cttgcaagtt tatgatttga attggctaat gcctgtatta
    15481 atactcttgt tattgctggg taccttaggc agcatgatca actggattat ttctccagcc
    15541 aagggcctgc tgatggcagc caaacatgat tttctgcccg cttcgctatg tcatgtgaat
    15601 aaacatggca tgccagcaaa aattttgata ctgcaagcca ttctggtgac agtattatgt
    15661 agcggctttc tcttattccc tacagtgaat gctatttatt ggttatttac tgctcttagt
    15721 acagagctgt atatgatgat gtatgtgatg atgtttattg cagcctggca tttgaagagt
    15781 aaattctcag acatggaaag accctttgct attccaggag gcaaacccgg ttattacctg
    15841 acatgcctgt taggattact aggttgtact gtcactttat gggtaggttt tattcctcca
    15901 acggaaagca ttaatatcgg aggaacagcc cattaccgta tggtcttttc attaggcatc
    15961 gtcttaatgc tgctacctgc tgccttgatc tatttgcgta aacgccacat taccaaatcc
    16021 aaagcgctta atgcacctgt ttaaatcatc gataggatgg ttgtaacccg tggcttgtcc
    16081 acagggtcag tgttttagcg gagagctcct ggatcccgtg gacaagccac gggacgtcga
    16141 agatcagtac agtactgggt tatcagagat ggattaatta aaataaggct tcaatgtttg
    16201 ttgtacatca agatttaatt cgccagtatt cggattgata gcactaaccg tatcacctaa
    16261 ataccctatc gcccagaaat ataaattatt ggacccgatg ccgctttggg taagataagg
    16321 catcaatgcc tgggtaaaaa agttttttcc ttcaaaagat ttccaggagg cttcccctaa
    16381 aacaatagga tacccagctt tgtacagaaa accccagctc caatcgaaac gcagattact
    16441 tgcatcacca ggggctgttt cccaaccagc cacttctttt gggtaaacat gaggtgagaa
    16501 aaccagatgg ccattatttc ccatattatc atcacccaac agccaaccga gtgtttctgt
    16561 gtcaatgccc ggaaccgcca gttgtaattc attactaaat ttttgtacat caaattgcgc
    16621 tatgttttgt ttagccaaat tcttatctaa aaggggttta aaatcttcac cccaatttcc
    16681 tttgaatgcg acaacctcct gatcattgcc acactgccct ggatcacgcc agtgtgcata
    16741 gccagaatcc tctttaatat tggcttttgg catgcaaatt gtaggattgt ccatatcatc
    16801 atttccgcct ccaggtcctt caacaaaaag caacaaatca ggattgacct gatgcacagc
    16861 cttggcagcg gtgcctatca ctttaatcca tgaaacttga ccatttctgg ctttaaacca
    16921 gctcaattga taaggttcat taaaaacatc aataccgatg acattatcca acccgcgctg
    16981 tttaatttcc tgagctagca gaactaaatc cttctcatat tgagtcatgc tataaacagt
    17041 tccatccctc attccatctc cataaccata acggtggtga aaatcaatca ttacattgat
    17101 attatgtttt ttcatttcag caagtaccgt ccagaaagcc tgcttcggac tcactgcttt
    17161 ttcacacgat tggccattgg actgccaggt cttgcaaaaa agaccattac cggtttcagg
    17221 ttgtgttttg tcagaaagcc atttgttcag atcaacttcc ccactgtcat cataaagaac
    17281 acctggctgt ataggcaaac gtacggtctt gaactctacc gctgtgctgc tgtttacacc
    17341 agagtctgcc atagaccagg ggtgaaccaa taaatccatt aatccattcc gagatttgga
    17401 ttgaggcatg gtgatattat agaaaggatt gctctgcaat ccctgaaaaa cattggtatc
    17461 ctgaaatcct gaccaggata ctccattgat ggtcacccta ttcccacttt tgtcatagat
    17521 actgccttga ctggtataat aggcattcgc atccagggtg aatacaaaca gcaaaagcca
    17581 actgctaaac ttaaatattt tcatgcgctt ttcctcatta aaataaccat aaagttattg
    17641 ttaagttggt taaaagctca ttatttaacc ataaaactgc aatatttgaa cagtgtaatc
    17701 attaattttt caaaaatatt acaaaaagat gtcatttcga tgtgctgaaa aatagtcaag
    17761 atcgtcacta cattatgatg aaaattgagt ggcgaccata cggagtgaat aaaatcattc
    17821 cctcatagaa aaaacgataa attacaacga gttatacatt gaaagtatca agaaaatatc
    17881 gctgagccaa aatcaaatct tcttttgtat taatatcctg taaaggttct tcgcaggcct
    17941 cctcaacccg gatagagaaa cctgaccata ggatgcgcaa ttgctccaaa cactctattt
    18001 gttccaaggt acagggcggc cagctaacga attccagtaa aaaggcagca cgataagcat
    18061 ataaaccgat atgcctgaag gtattggaat aagcgttttt atcatctctg tgaaaaggta
    18121 tcgcgcttct tgaaaaataa agagcgtgat tatctcgagt gcgtaccact ttaaccacat
    18181 tagggttatt taatatttga aggctgctaa taggccagca taaagtagac acaggcgcct
    18241 ttgtctttgt taaagagcaa gcaacttgtt gaattaattg gggtcggata aatggctcat
    18301 caccttgcac attgacaatg acgtcatcag gagcaaagct acccttggcg acaacctctg
    18361 caattcgatc agttccagtt tggtgcgtat gtgaagtgat tacaacctcc gctccaaatt
    18421 ggattgcacg gtcagctatc tcatcatgat cagtggcaat aatcaccgac attggttctg
    18481 ccagaagtgc ttgcctgtaa actcgttcaa tcacggtaat gccgttaatt tcttgaagta
    18541 atttacctgg aaaacgactg gagtgatatc tggctggtat aattacatga aaattatggc
    18601 tcataattta tctttctctt ccagaggaat caaacgcgct tcttgttcca gcataacagg
    18661 aatatcatca cgaacaggaa atgccagcct atcaaatttg cagatcagtt catgtttttt
    18721 aaacagcaat tttcctttac ataaaggaca aaccaatatt tccaataatt ttttatccac
    18781 gcgaatctcc ttgagtaacc tgattgcgca catacactgg ctgtgcctgc gccacagaaa
    18841 ctgccttgat atttgctttc tgtgccaaac gtatcatagc agacgctgtc ggaaaaacag
    18901 ttaatacttc actaatttgt gatttgatct gctctgggaa atccttccag tacaaatcaa
    18961 tcccaactcc ggccaaaata aaggattgat tggctggcag ctgaatatct tttactgcgt
    19021 taattctgtc ctgggctaag aattgctgct ccaaaaagca actccaatac atttcatgca
    19081 tacgagcatc caaaacagat aacacaggct gattaaaatc ctcttttatt tcccgggctg
    19141 tccaggcaat tgcggctaaa ctgctcacag gaacaagagg caaatcattg gcataagcta
    19201 acccttttgc aatgctacac gcgattctca atcctgtaaa acttccaggg ccacacccaa
    19261 aaattattcc atccaattga ttaagaccta atccggtttg ggctaccaat tcatcaatca
    19321 taggtaaaat caattgcgca tggattcttt gactatcctg ctccctgctt atgatttcgt
    19381 catctataag ggtggcgaca ctggccagtt cagttgaggt atcaatcgct aacagtttca
    19441 tcatttaaag ccaactcttt aataaaatgc agtacttttt cttcatcccg agttttcgga
    19501 agaccaggta aacttgccaa aatagttcga ccatactctc taccagtcaa acggggatcc
    19561 gctatcatta atataccttt atcggtgatg tcacgaatca atcgccctac tccctgtttt
    19621 aatgcaatga cggcatttgg caaggataat tcatcaaaac cggaaagccc ttttttcttt
    19681 aaataagaca ttctgccacg aattacagga tcaacagggt tagcaaaggg taatttatct
    19741 ataatcacgc aagaaagcgc ctcccctttg acatcaacac cttcccaaaa agtagaagtg
    19801 cctaaaagca cagcattgcc caattgcctg aatcgagaca ggagaatggg ttttgcttca
    19861 tcaccttgaa tcaaaagcgg atattttaaa gtattgctca tcatttgagc aaccaatttt
    19921 actgctttat gactggtaaa aaggaaaaag caacggccac cgcaagcctc gataacaggt
    19981 aaagcctttt ccaccaaagc gtcataataa tgagcatcct taggatcagg tattcctcga
    20041 ggcaaataaa gcagtgcctg ttgctggtaa tcaaaggggc tggataataa caaagtctgc
    20101 actttatcta atcccagagg tttacaaaaa ctatcaaaag ccgatgccat agtaagcgta
    20161 gccgatgtaa aaatataggc acatgactgg tgctttaata attgactaaa tgattcagca
    20221 acgtcatacg gggtggcatg aaaaacgaga gtgtgtttaa aacgttccaa ccaacgaatt
    20281 ttattattat tttcctcagt aaaggaaagc aagatggttt ccaaatccaa aaggcgttct
    20341 ttgcatcttt ttaacctggg aatatccgtg gcattctctg attccaggca ttgaactaac
    20401 tcatccttta aagataacca ggtatcccac gcctccataa actccttatt tcgtttaacc
    20461 aaatcccaac tgattcgttc ctcacgagtc ggcaacgcat tgagcagttg atccaccagt
    20521 ttgtctgctt tatgacttat agactttaga ggttgattgg ctaaatccag gattggccac
    20581 tcattaagca tatcagcgac caagtctttg aattgacgtg ttcccaggcg ctctccattg
    20641 aaattagagg cgatgtctgc aagctgatga gcttcgtcaa acactacgac atcaacacca
    20701 ggtaatagtt cgctaaagcc ttcttttttt aactgagaat cagcaaaaaa aagatggtga
    20761 ttgataacga caatatcagc atccatcgct cgcttacgcg ctttcactaa aaaacaggtt
    20821 tcatgattcg gacattctgc accaagacag ttatcggtag tggatgtgac ataatgccat
    20881 actgaggagt cttcattaat ttcaggtaat tcagagcgaa cgccttcttg catttgagat
    20941 aatttatctc tgacatggag tatttcgtga gcacattgcg ggctttgaaa ttgtccttcc
    21001 tcagcatgct tctcaactcg atattgacaa atataattgg aacgcccctt taaattctgg
    21061 attcgtaccg ataaacccaa agctctgatc aaggttggca aatctttttg aaaaagttga
    21121 tcttgaagcg ttttggttgc tgttgagata agcgcttttt taccacttaa caaacaaggc
    21181 aataaataag caaaggtctt acctgttcct gttcctgcct cagctaccaa aatcgatcgc
    21241 tgaacaatag cattggctat agccactgcc aaatcggctt gtggtgctct tgccacaaag
    21301 cctggaatag cagttgataa tcttcctttc tcactgagag ttcgccgaca ggtttcagcg
    21361 aaagtggtta tttcttccac tctttttgca aatattgaag tttgtccttt acttcttccc
    21421 aatccttggc atcagaagga gcgtcctttt tagcagtaat attaggccag gttttggata
    21481 actcggcatt taattcttta aattgctgtt gttcctctgt taaatcgtct tctgaaacga
    21541 tcgcattaac tggacattca ggttcacaaa gggcacaatc gatgcattca tcggggtgaa
    21601 tcaccaggaa attaggcccc tcataaaaac aatctacagg gcatacttct acacaatcgg
    21661 tatacttgca tcgtatacaa ctttccgtta ctacaaaagt catagcaaac cttaaaaatt
    21721 atctttaaga aatgactcat attaaagcat aaaataccct gctgatgata gaggagcctg
    21781 atatttttat accacctgga ctctattatt tagaaccaat ctgaaataac aaatatcaaa
    21841 ctaacagcct cgtatatttc cttgctgatc atataataca cttgaattct caattcaaat
    21901 atgttaaatt tgttcatttt aattatattt tgatcaatag tttgatgcac agcctgtgta
    21961 attagatacc tcggagtact tcatgccttt gaatttaccg cctaaatcgt ttaaagatat
    22021 aacacctttg gtcattgcgt acaataatgc acctgaagat gacaagattc aaaaattatt
    22081 ttatttgcaa aagatcaatt atttactgaa taaaactcaa ctaaatgatg atttgttcga
    22141 ttggataaac gatgcggaag aaggtggctg gttaaatgag ctggctaaat tttcaattaa
    22201 tcccaatgca tccttttttt taaaaggaat gcagtttgcc aaagccatta ctgaagaaat
    22261 aaagaataag cctgaaatta acagttctga agtaaacatc taccagctca tgcaagagcg
    22321 tgaccagctg ttaaaagaag tagaatttga aaaatgtgct actcgctatg ctgaaattaa
    22381 ttttctcctg aatgaattag cccttaacga taaaaaaaca aaagaaattg tagaaagaca
    22441 aacagaaatc cttaggcttg tcgctcccaa aataaaagcc attaagggag agtccataga
    22501 caatttgcca gttattccct cttataaaac caaagaactt ggtaatcatg tgaataattt
    22561 taacttcaaa ttcacaatga gcggatggga tgcccctttt gtatttcgtg tagaagacag
    22621 acatgagtta ggaaaagaac aagagcttca ttcctatggt gtatccaagt actttattga
    22681 agattactct gtttttatga tgcgatttaa agcggaagat ggttctactg tgtacaaacc
    22741 agttatcctc agtcaattcg ccaatcaaaa taatttggaa gaaatcgcca aacagttaaa
    22801 agatggtagc cccaaaaata tcgctccgcg aattggatat tactttgttc agttaactga
    22861 cttttgtcta aagttaatag aaactcataa ctatcaccct gacattaagt taaataattt
    22921 tctggttcac aataacagag tactggtgag cgatagaaaa acattcacca cgagtgacaa
    22981 tcctttggcc agtgacattc taaccagtcc tttatttgca ccggatgagt ttttaaaatg
    23041 tctactattt aacaaagaag gcgaccctgt aggctataat cgcaatgctc tgtggaaacg
    23101 catgaacatg ccacaattca tggcctatca actgggaatg gctttaaaac aattcctgat
    23161 tttaacgcaa ttggatgaat taccagatga ttttagaaat ccggatcaca gcgccgtatc
    23221 acatttcaaa acaccatcca gacaaatcat caatctttcc cttctggttc aggaattaac
    23281 acgcctcgat cctgataagc gaatgaccat taaacaattc cagaccttgc ttaacttcaa
    23341 aaacttaccg cctgatgcct tttaccagaa agtggaagaa gttttcccct cttcccaatt
    23401 agggatcgca gaagatattg aggctcttaa taaagtattg aacagcgatt taaaaggcga
    23461 agcgcttctg aaacaagcta atcctgtttt cactaaacta tcaaaatatg atcccaaaga
    23521 gacccgatta actcgtctag ctgaaaaatt ggcaattcga tgttttaaca acgtgtcaaa
    23581 accttatttt cagaaactgt ctggtttgat tgaatcagcc ttagaaaaag atgttaatga
    23641 aatcagacaa ctattaaatc ctcaatcaaa tagcgagggg cttttacaga aagccagccc
    23701 catttttatg aaactctttg aaagacagcc tgaggtaccc caattaacag accttgcgaa
    23761 acaattagcg gctcaatgtt ttgatgaatc ttcaaaaaac tattttactc gtcaactgcc
    23821 tttattggtt gaagaggaat tattaaacca ggattgggaa caagctccct ggtatagaaa
    23881 agcactgcat tggttaacct ttggatactt cagagtcgat aacgtcacag aaatatcgtc
    23941 cttgaaaata cccgagcatg caaaaggtaa agaatttcag atttattttt cgcaactctt
    24001 atttttaccc aatgaagaaa tcaaaaatat gtttacttcc aaagacagtc aaacagtcga
    24061 ttttctcctt ggttatatcg aaaaggaaag agaaagaaga gagatggaaa ctccctcagt
    24121 caagattcct gttgatttaa aaggagaaga gtttcagatg cacttcccac agcttgaatt
    24181 tttaccttcc aaggattttg aaagcattgg tgaaaaagag ggtgagcatc ttgaatgttt
    24241 tatctttgcc aatcttcaag aaatactatc gcataataac agtgattcat cagaaaaaaa
    24301 tagtactagc gaacccactg acagcgaaag tgaaatagat ttaaatgttg gcaacgcagc
    24361 aggaacaatt ataattgcgg acgaaccaaa tcctgctgaa accaaaccga agaagaatga
    24421 caatgatgga ataaaacaaa gtaatcctgc caaaaccagc cctaaaggaa aagtttcaac
    24481 tccagaagtt gatgagcatc ctaaaaagaa aacaaaagat gatgatactc ccaaaccgaa
    24541 aacaaagcgt tctgtacact tctttgatga ggctaaaaaa tccaaaccca aggagaaaga
    24601 aaatgcctct cctactgttg acgaaacgcc ccgcgcgaag agaaaaaatg tctgcaggat
    24661 agatagcgtc aggtccaccc ttttcagagg agatggcagc cataggcaga aattcaaaga
    24721 acaaagaccc aggttatctg aaatagcttg ggagccgcca aaacctaatc tacaatagca
    24781 aacaaaggcc tgcttcttga ggttttctgc agccaccctg attatacagg gtggctaact
    24841 taacaaatgt catggtggtt tgaaacttta ttttctcttt ttgttaaaaa attatacatg
    24901 tttttaacaa cagcataaaa ctatgtctat atttatagaa aagaatatac aaaaatcaga
    24961 gtcaaaataa tgacttattt ttgactgtga agtaattaaa aataactaaa aatttgaagg
    25021 aaaacattga ataggaaaag tatttttata aatatggaaa taagtctaat tgatactata
    25081 aggaaatagg caatggacgt tggccaaaag ataaggattt taaaacgtta tttaaaagaa
    25141 aaaagtgtca cgccagagga cgtcaatctc aatgatcaag atctaacagc ctatgccagg
    25201 ctggataagc tattggatta tatttctata cataacaatg gattgtttga cttcacaaca
    25261 ttggaatctg atgaaaagca atggaaacaa tatgtggata tacttaacta ttccgcaacc
    25321 tgttcaccag gctctccccc tgctttaatc gataccagtg aacgcaaagc tcaatttggt
    25381 ttgcaatatt ggctgaatac gagaacgatt gaagcactgc caagagacaa ctattatact
    25441 cgtacagtta ccttatccga agaagcagat gaagaatttg atcagctgat tacagaaagc
    25501 agagccaaat tgtatgagat agcgcaaact tttccagaaa tcagctcttt taatccagca
    25561 aatattatcc aggtaaccaa ccaaatctct tcgattaaag aaaaactgga tactttgaca
    25621 agcaataatc ctcaaataac ccttttacaa aaaaaactca acgtactaac agatttaaac
    25681 aagattaatc aactcgagag gctcaatatt ccccaagaca atggttctaa cttgagttat
    25741 tgtacgctcg cagagactat tgaaggagtt actgagaaaa tacctttaat aacacttgtc
    25801 aaagtgaaag agcaattgag acatctttgc gctcaaaatg agcttgattt ggatagttat
    25861 agtgagtcaa gagaacaata ttataaactt ggtatagaag ggatacttaa attacccaag
    25921 catggcgaca taaaagaagt acaacgtgaa gccatggctc ttaacatctc aagattaatg
    25981 ggattagaca ctacttcttc aacaacagta agctataatg gccatcccgc gttgtttatt
    26041 ccttttgaca gcatcaatct gttgaatgaa ttttcttcag gtaaaagttt taaagctggc
    26101 atgggattaa gcgggcaaag ttatacccat tactccacaa ttaaaccatt aggtgaagga
    26161 atccaggccg atctttatat caaggatttt ggtcatgctc tggcattact ttatttatgt
    26221 tgtgatacag atgctatagg tgggtattgt aaaaacaaag cattaagaaa ctacagtagc
    26281 ctctatattt ttgatcaggt cattacggac actgataaat ttatccttga ttcacgaatt
    26341 agtcttcaac ccgatcagtt cattatgaaa catacccgtc atgggcaagg gagaaacaga
    26401 accttaatcg aggattcttc cttagttacc aaatacgcaa gcctcgtgcg acttaaagag
    26461 ttaaaacgca ttattaccca atactgtgat catatttcat ggctgcatca taatcgtact
    26521 caagccatta aaaaacaatt gcaagaagaa cttgcaaaag acgaacgcgt acgattaaca
    26581 gaagagttaa atgatgttga aattttggaa aacgatgctg agcaaataaa aagccaaatt
    26641 ctaattcgca tcaataaaat agccgaggtg ctacctcaaa cgactgggcc cgttggctcc
    26701 gatgtcatcc gtcaatgttt aattctggag aaattattac acaacccagt tctctactct
    26761 gatgatggaa gaccttataa aaatccatgg acaacacgac aagcgaatac agcacaaacg
    26821 atcaatgatt tacaaaacgg gaagatacaa attactttta aattcaatat cccagaagaa
    26881 atggtcgact ttatcaaacg ccatggtggt ggaaattcac tgactatcag ctctcctaaa
    26941 gtgataacga ttagcaaagc ccatctggat ggccttaggg aaaatatgtt gcatcctgaa
    27001 cattcacctg aattgtctct ttatactaat tatttagacg ataaagacct ctgtattata
    27061 aaaatggctt atcgtatagg taatagaacc aaaattttaa atgctattgc agaataccgc
    27121 gcccttatga ataatgaaca agtaagcttc cataacaaaa tagaatgtct tgcccaaact
    27181 gaagcgaaaa tcaaagcgct gatagaattg gcaaatgacg aaggcttggg gatgcacatt
    27241 cttaaaaaat tctattttga tgcgcagcaa cgactccaaa aattaattaa tccagaagtg
    27301 ttgccccatg acattggtca agcctttgcc gccgctctta ggctggacag agtatcagaa
    27361 ttcaacaagg ttgtgagaga agctattgtc caaaataaag tgactgaccc tcaatttgca
    27421 atttttttga caagttgcat caaaattgaa tttcttgcca aaaattataa agacgcacaa
    27481 aaagcaagca gagccttatt aatcgagtct cagcgaatta taaatcatct aaaatcgcct
    27541 gtagttcctt taattgttca attaggaggg caattgcatt cacgggctga attagatacg
    27601 gatgggttag ccaatcttga aaatgaccta cagcttgatt ttgagaaacc agatacagaa
    27661 tcaatgaaca ctatggattt acatcaggct gttacgaaag aagaaatgat tggagatgga
    27721 atgacagtaa aagtttaata gatttctttt aaaactttct gtaatacagc aaatgaaaag
    27781 agagatgttt aaacttggaa taatggagtg actatgccag tacatgaaag gcgtcaacat
    27841 tttcgtattg atgatcatat ctattttgat tatcgaattc ttgcaccggg tgaattttgt
    27901 tccgatatag aaatcactaa tcagttacta ggtgaaaatg ggcaaaagta catggaagcg
    27961 gcgcaatatt ttcagggaat tgattatgag cttgccgagc taactcaggt aatggcggtg
    28021 aaagaacctg ctttggcgca ttaccttaat cttttgaatg ccaaaattga ttatttatct
    28081 cgacaaatgt taatgtcgag taaagttcaa ctacgtaaag tcaacatcag cctgggcggc
    28141 atggcattta aaacaatgga gcagattaga gaaaaaacca atctaaaaat cattctgtac
    28201 acaaaaccca aaatgatacc cattattgtt gatgctgtag tggtttatag tcaatatcaa
    28261 agtgagacgc attaccgaac agcggtggct ttctgtaatt tgaccagtga gcaagaacag
    28321 ttgctttcac aacatatttt acaagcccag ataaaaagcc gtgctgacta agatggagat
    28381 actatagatt ggaactattt tttctaggat aatgagcctt caaattaaaa taacgaggtt
    28441 atatccatga gcagaacagt attttgctgc aaattaaagc aagaagcaga aggcttggaa
    28501 aaacaaccct tccctggtga attgggtaag aaagttttta atgaagtatc aaagcaagca
    28561 tggaatatgt ggctttccca tcaaaccatg ttaatcaacg aataccgcct gaatttgatt
    28621 gaagccaggg ctcgtgagtt tttgaaagaa gaaatgcaaa aatatttttt tggcgaaggt
    28681 tcagataagc cctctggcta taaagaaata aaataaagga ttaatttatg tttcatgact
    28741 tgttggaatt taacgaagaa gtactggatg ctatcaatga taaaaaccca attgttgctc
    28801 ttgagtcgac cattatctct catggaatgc cctaccctga taatttaact acggcaatag
    28861 aagttgaaaa tattattcgt agacaaggcg ccattccagc tactatggcg atgcaccaag
    28921 ggaaaattcg tattggttta actcaagaag ttatggagca tctcgcgctt caaaaagaag
    28981 taataaaagc atctcgcaga gacatttctt ttgtattatc tcgtaaagtg actgccagca
    29041 cgacggtagc agcaaccatg ttttgtgccc atatggctaa gctcccccta tttgtaaccg
    29101 gaggtatagg tggtgttcat caagacgtga cgatgagttt tgatatttca gctgatctta
    29161 tagagctatc caatacgcca gttaccgtag tctgttctgg cgcaaaatcg attctcgact
    29221 taccaaaaac attggaagtt cttgaaacgt ttggggtgcc ggtcataggt tatgccactg
    29281 atgaattccc cgctttttac agcagatcga gtggaatacc agtcccacaa cgcttaaact
    29341 cagtcgagga agtagcaaat ctcatgtcaa ttcaacagaa attgaacatg aaaaacggaa
    29401 tagtcgtcgc aaaccccatt ccagtatcag cggaactatc tgatgaggaa atcagtccat
    29461 acataaaaca ggctcatgaa gaagccaaac acatgagcgg aaaatcatta acccccttcc
    29521 tgttaaaacg tattgctgaa ttaactgcgg gcaagagcct ggaagctaat attgagctta
    29581 tcaaaaacaa cgcttttcta ggagcagaga ttgccatagc ctaccaaaaa aaattatttt
    29641 caaaaaaaac ttaatcaaca cttgactcaa agtcgatctc ggagtttaat agcaagcctt
    29701 gagtggccag atagctcagt cggtagagca gaggactgaa aatcctcgtg tcggcagttc
    29761 gattctgcct ctggccacca ttagaattcg ggtctttccc aattacggag gcactcacca
    29821 tttaacaaca caacacccta acacgagttc tataattatc aaagttacga aagccataag
    29881 ctcgtctttg aatcagtttc atctttctgt gaaagccttc tgtaatgcca ttagattttc
    29941 taaaccgcca catacgaaca attatttgat ttgataatgc aaacgatata caatatgtgc
    30001 aattttaatt catagatcaa gtaaattgta cccattcatc cttaagccca aatccttgcg
    30061 tttttttaac cttgctttta ttgctttaac cctgtctttc tgtttattag ctaatgctcg
    30121 agcggcagga attgaacgct atgacttcag agatatgacc tcaaaggtga ctaaagcttg
    30181 ggatcaagat gttgcgcttt atgttgccaa gcgttttgca gtagaaaata gttttgatga
    30241 aagtgagata acagtcaaaa aattaaatgc aggggcgacc aaccatttta tatatttgat
    30301 gtttatcaat aacgagttga agtttgttat taaaggtctg ggaacagaaa aagaagcaga
    30361 ggcattgttt gctttacaaa attactcaaa aattcctgat atccaaaata atccacaagc
    30421 cgcgactgtt acattaagta aaatcattaa ttcatataga gtgaagaatg attccagaac
    30481 ttatttcttt gcaattttag aagcagctca aggaaaagag ttatttaaga taatgactta
    30541 tgaattgcta cagcataaag atattaacac tttaaaaggt gttttttatc gaatagggca
    30601 acagatggct gaattacata aaacaattat cgggagcgat caatttatca ctccaagaga
    30661 tttagtgacc gttatacatg atgattttca ccctgaaaat acgttttata atcttgaaac
    30721 aaatatcttc tctttaattg ataatgaaag catgggcgat agtattacag agcctttgcc
    30781 aattatcaga gaattatatg ggttttatga agttcctatt attcgctggc cctcagaaaa
    30841 aataactgta gatctcttaa aggatgctga tcctcatgat atagctagta tctattacga
    30901 ctttgttctc ggtatggcct cagtgtatca agacccccaa gatgcaaaac aagtgttaat
    30961 tgaagctatt attgatttaa ataatatggc tattgccttt ctgaaggata gatattctga
    31021 gccatggatg cttaacccaa gcgataaaca tgaagcacat attgtgtggg gcaagttagc
    31081 aaaagtcgac actaatccac agctagcaga aatctatatc actaaattaa ccgttattaa
    31141 taatgactta caatcacttt caggccttaa acactagcat agggtgggag ctcaggccga
    31201 tcctaaccct cttttaatta cttaagcagt ctttcatcat ttgtttaaat gtaataatgg
    31261 agctgaattt taattactct atatactgat gtagtatgag cttattttag attatttact
    31321 tttggttttc acaagccgca taagacccta tacatcatct acttatctat ttatttaaag
    31381 tgtatcgctg ctttatatct ccttgctcat tgataaaaga caccttggta aaggatgaat
    31441 catctgacta acttccaaat tccacaagaa atttgacaaa ctataaacag acttatccac
    31501 agaaaatgtg gataacttaa acaaaattac aggtattaaa aaatgatatc aaccaagccc
    31561 aataaggatt gttatagttt actgattaat cggttgtgat ttggtaaaat aagatatcca
    31621 caaacaacgg ataaaggaaa atgttcttga cctacctatc ttatacaaat aaaaaataaa
    31681 aaaacagtct tgactcgaac tatttttact atttattttg acccgctgct tgattaaact
    31741 ttttgcttaa acaataactc ccctgcctta gtgaggtttt taaatttgga actataacca
    31801 tcttgtctgg aacaactaga atagcgaaca agcctatgcc tgataaatag acaaactaat
    31861 attatgtgga gatggtttgt tcaggtgttt taaaatcggc aacgtatcca ttcttctttg
    31921 gatttcaaat ctctaataat tttggcatgg ttgcttaatg ttggtgtccg gcttttcgag
    31981 gaaggatcag tctgagatgt tatgacttaa cgcatcacac agtttaaaga ttgaaaaatt
    32041 tgcgagttac ccaactcaat tcaagctatt gtcgaaatta gcatgatgca attactaaaa
    32101 tatcaatata ctcagacaga tcaatttctt ccagattata cgggtttgtt ttccaaattt
    32161 cttttaatga atttttatag gtatccgtat attcaataat gcgggttccc tttttgttta
    32221 gatcactttc agtataaaaa acatctctat ccgctctttg ttctatccac attctgtaag
    32281 gcactccacc actttgtgat gctgtgcata aatcagtata tgttgtaata gtttgaaaat
    32341 tctttaaatg gcgttttaat tcatggcgat aactttcaac tcttcccatc atgcaagaat
    32401 ctatatctaa attttttagt gatatttctt ttaactccaa atcattttca agaatatcta
    32461 ccaactcctc agcatcgcgc cccgcaatta attgcttatc aatatcaccc tgcccgttaa
    32521 taagtatgga atcgccatga cttaatcgag ataataagta tttaatatta tctggttcat
    32581 aaagattgat tattggttgg ttataatttc ttttcctggc ctcgtttaaa accagattaa
    32641 atttttccga atcaggagca tagataaatc taacatcttg gccatatatt tttgttaaaa
    32701 gtgggccaga atctgcacct gaagttatct catgattaac ttgttgcgca tattcattac
    32761 tataacaata aagctcaatt acatgcgttc gatttgattt aaaaaattta gccctggaca
    32821 taatatcttt taataattgc ttaaaaatat gtcattttag catttaagaa ttatgatttt
    32881 attaacctat ctatcaaaaa aatgaattta cctcggtgta gaacaaaatt tcatatgcca
    32941 actgttgttt tttttcagta ggcaaatttt ccgataattt ttctaaactc agcgactttt
    33001 attgcaaagt tgctgagttt atctgaataa atcaaggtag tatttgaaga aactgcctca
    33061 tggatctttg tggtttttat agttatccac ttaatttggc gtttatttgc ctaccccttc
    33121 cctatgttat gagtcctgac aaagcaatga tcataaaagc gagcattttt ctaatcgtag
    33181 agaaagattg ttctcatgca cttgaaatct tggttaaatg gagcgaggat tataacaatc
    33241 tctttactta agctaaaaaa atgaattcat atgcgaccaa agtcaccccg gtagaagagc
    33301 acattgccca gaccacttct ccacaattta tgtctatatt tttgttagca caaattttaa
    33361 gcatattgat tggtgttgcc tgtctttttg ttgcatttta tatacttcgc ccaagaaaaa
    33421 aagaccaaat gctcatccag gatcagtttt tactttattt aatcctggga ctttttttca
    33481 ttttttcccc tttcctgctc gaatatttta gataattagc aaaagttgtc gggccaaggc
    33541 ccatagccgt caagataagg gataaatttt tgacttaata tattgaatta aaagaaaaaa
    33601 aagctagagt accctttaaa aatttccgcc aagaaaaaga aaaagggact ctatgctagc
    33661 gaatacaata ccaaatttat ctcaaaaaat cgttattttt ttacaaaaga agctgaacga
    33721 ttgggcaaag aaagcagcta taagcgacgc tgttcaaaat tgacccccag cgcttttata
    33781 aaagcgttgc tgtcgactag tttgtgtcct tattttaatt tagaagtgtt tggtagcttt
    33841 ctaaataaac agaaagttag tattaccaaa caaggaatac atgagcggtt taataagcaa
    33901 acagaaacat ttgttaaagt cttatcgacc tcttttttaa actattttaa gacagaaaaa
    33961 ttacctaaac tgggtcaatt agagatattt actagtctga atatcattga tagcagtagc
    34021 atttctttac atagttcgct gagtcaatta tttaaaggaa gtggcggagc tgcatccggt
    34081 gctgctttaa aaattcaatt gatgttcgat tatcttatag gtcaaataaa agaactgaca
    34141 gttacatcgg ggtgtgataa tgatcaaggc tttgatgatt attttcactc catagaagca
    34201 ggagctcttt atctaatgga tttagggtat tttaaactca actcatttaa aaaaatcaga
    34261 gagggacacg ctttttttgt aagccggctt ctcataggca ccaagttatt tacattagaa
    34321 aaacaccctt tagatttgct tgctacctta tctacagcag agagttcatt ctcacaacag
    34381 gtgcttatgg gggctaaagc tcaaattcct gtgcgtttaa taacgcaaaa attacctaaa
    34441 gaaatcgctg accgcaggcg acaaagactg aaagaagatc ataggagaag aggtaccaaa
    34501 ctgagcaaag aagccctcgc tttac
//
LOCUS       DBNCWK010000021        32795 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.021, whole genome shotgun sequence.
ACCESSION   DBNCWK010000021 DBNCWK010000000
VERSION     DBNCWK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 32795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32795
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.021"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..1241)
                     /locus_tag="AC06IO_000893"
     CDS             complement(<1..1241)
                     /locus_tag="AC06IO_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HIO9874103.1"
                     /translation="MPSTVIILLIILIAIALRQVVRVIIPIWAIMATGAIAVLLCQQI
                     TPSRAFAAVEPDVMLYLFGVFFISQAAEESGYLTFLTDQIFYYANNGKQTLMLIVFIL
                     GLSSAVLMNDTIAIIGTPIILQLCQSHKNLIKPLLLALAFSITIGSTMSPIGNPQNLL
                     IAIKGEMASPFFEFFKHLAVPTLLNLGIVYFFILFLYRNELKEPIKKPVLPLINDRSN
                     LTLVKMSLLMMLVLTMIKILTDFLHYSEFQLNFSYIALLSSVPIWFSKHRWNYIKRLD
                     WGTLVFFASTFILMQSVWDSGFFQTGMSDSHIDVSHIVVIFIMSIALSQLISNVPLVT
                     IYLPLLMSHNHSNITLLSLAAGSTIAGNLSILGAASNIIIIQNSEKRGIRGFGFLEFI
                     FVGAPLTLLNLLVYVSFLQKI"
     gene            complement(1258..2643)
                     /gene="glmU"
                     /locus_tag="AC06IO_000894"
     CDS             complement(1258..2643)
                     /gene="glmU"
                     /locus_tag="AC06IO_000894"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIO9874104.1"
                     /translation="MNLQIIILAAGQGKRMYSDTPKVLHHLAGKPLLTHVVETAQQLN
                     PDAIHVIYGHGGEQIKSSLPNLPVHWVHQAEQLGTGHAVLQAMPHIPDDAYVLVLSAD
                     VPLIQVETLQSLIECSQRQNPDHSVLALLVAELENPSGLGRIIRNNQGEIYSIVEEKD
                     ANEQVKNIKEIYSGVCCTLANNLKKWLPQLSNSNAQGEYYLTEIISLAVQNKTPITSL
                     TAKNSFEVQGINNRQQLQQLERIWQQRAANQLMEKGVTLADANRFDLRGKLYCGKDVY
                     IDINCIFTGKVVLGNGCKIGPNCSLTNVTLGDGCEVYANSVLEGCHIANDCHIGPFAR
                     LRSGTQLAPHCKIGNFVETKKAIFDEGTKASHLSYLGDVLLGKNVNVGAGTITCNYDG
                     VNKHQTIIEDGVFIGSDTQLVAPVTVGANATIGAGSTIRRNVPPDELTLTESRQKTIY
                     GWKRPVKRERD"
     gene            2765..3649
                     /locus_tag="AC06IO_000895"
     CDS             2765..3649
                     /locus_tag="AC06IO_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2874 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874105.1"
                     /translation="MNIFLKVLKKLMKEKLSELSANLKWKSETIGYNTKLSIDQKNVI
                     DKLEESIVLFKDSGNDEADLLSINALIDAARIQIQSIRESYGEPRDKGESVTCLNNLK
                     NHSNDFLGKLKKFDFDLLNKAYTETPENIVYYHAAIYLGDEIFSPRTGIDYEIRTKKE
                     VQLAVRLQALSERINPSHNLEEQRKRALQVLQDLAQDNQNAIKKDKGFSLPGLSFWGV
                     SVVTPSDWFSSGEGRFGVEFNTAVRTIQGMTESQFERPTVKQTTAKQEETKPSDVKKT
                     EATDEEDEEHSSENTHTL"
     gene            complement(3747..4757)
                     /gene="ansA"
                     /locus_tag="AC06IO_000896"
     CDS             complement(3747..4757)
                     /gene="ansA"
                     /locus_tag="AC06IO_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIO9874106.1"
                     /translation="MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQD
                     MPDYVIKEYQPLLDSSNMTVTEWNRIARDIAHEYEQFDGFVVLHGTDTMAYTASALSF
                     MLEYLGKPVIVTGSQIPLSEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRT
                     QKISAQRFNAFDSPNYPPLASIGIDIELHQHRLLPRPKAPFHLQTITPHFIANFRLFP
                     GFASDVLEYILNQPLRGLILETYGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQG
                     KVEMNQYATGHTLKEAGLISGHDMTPEAAHCKLLYLLSKNLEIPEIKKLIETNLCGEL
                     QI"
     gene            5110..5637
                     /locus_tag="AC06IO_000897"
     CDS             5110..5637
                     /locus_tag="AC06IO_000897"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIO9874107.1"
                     /translation="MVIDRAGYRLNVGIILVNDSDRVFWGRRSGHDAWQFPQGGLAPG
                     ETAMQAMYRELHEEVGLDKGDVEILGSTRRWLKYRLPKQYLRHGSEPLVIGQKQKWYL
                     LKLVTSEQKVRLDLSDSPEFDSWRWVDFHEPEQQVIFFKRQVYIQALKELEPLLKKER
                     RTPYGLKRKRGNQRA"
     gene            5638..7932
                     /gene="ptsP"
                     /locus_tag="AC06IO_000898"
     CDS             5638..7932
                     /gene="ptsP"
                     /locus_tag="AC06IO_000898"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIO9874108.1"
                     /translation="MLKILKRIVQDVTAASDLKEALGILVQRINTAINAEAVSVYLID
                     NKNAEYVLIATEGLNKQAEFRVRIALDCGLIGLIGRREEPINIENAPSHPDFYHNPLI
                     GEDHLNAFLGVPIIQHRKLYGVLVVQQLEQRHFDDAEESFLITLAAQLGGIIAHAEAT
                     GELAELTQPKKGIGVDKVEAAYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEE
                     LEDEINAFYEALETTRDDIRRLSKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVI
                     REEKISAQAALATVIKKHVQQFESMGDDYLRERASDFRDLGRRVLAELQRTQREEIVY
                     PKRTILIGEEITAAALAEVPEGQLAGVVSAKGSNNSHVAILARALGVPTVMGLRGLKV
                     ELVSRRALIVDGYYGHVYISPSKSLLAEFKQLAQEEQELNQSLVSLRDKPAETTDNYR
                     VSLQVNTGLAMDAGLSMSVGAEGVGLYRSEVPFMSRDRFPSEDEQTIIYRQTLKAFAP
                     RLVTMRTLDIGGDKILPYFPVEEENPYLGWRGIRITLDHPDVFLIQVRAMMRASEELD
                     NLRIMLPMVTTLSEVEEAVYLIEQAFEELVEEGCEIVKPKIGVMIEVPAAAYLAREMA
                     KRVDFISVGSNDLTQYLLAVDRNNARVAALYDPLHPAMLRTLLKIVEGGHAAGVEVSI
                     CGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRNFAIANARKILAEVLEFEHPA
                     EIRFHLQKALEEEGLGGLIRAGKS"
     gene            7929..8735
                     /locus_tag="AC06IO_000899"
     CDS             7929..8735
                     /locus_tag="AC06IO_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIO9874109.1"
                     /translation="MISGELLINGVIYIIIGAFAGIMAGALGVGGGLIVVPGLVYIFQ
                     HSQVIPEDILMHVAAGCSLAVMIFTSYASLKAHNKMDEILLPLFKKLCPGLVLGTILG
                     SIMASFIPTHLLKIIFGLFLLGVAMKMLLDVRVSHTDKFPGYWLNTLVSSSIGSISGL
                     LGVGGGVIIIPYLTYCGVPVRKIAAVSNLGTFAVGLVGTIMFMVTGTLDMTKISYSTG
                     YIYWPAVLGVAIPSSLIAPFGAKLNYLLPVNYLRYGFIVILITTAIHMFL"
     gene            8753..9523
                     /gene="lgt"
                     /locus_tag="AC06IO_000900"
     CDS             8753..9523
                     /gene="lgt"
                     /locus_tag="AC06IO_000900"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIO9874110.1"
                     /translation="MLTYPNINPIAFSLGPLKVHWYGLMYLIGFVGAWLLGYWRIKHY
                     KLNWNNDQLSDLIFYSALGVILGGRVGYMLFYDFQEFIHHPWVLFKIWEGGMSFHGGL
                     LGVVIAAWLFCRKYGKTFLEVGDFVAPLVPLGLAAGRLGNFINGELWGRVTDVPWGMI
                     YPHVDDQPRHPSQWYEFGLEGVALFILIWCYASKPRQQGRVSALFLMGYAICRLIAES
                     FRQPDSQLGFVAFGWLTMGQVLSIPMLLIGIWLWWAKR"
     gene            9520..10314
                     /gene="thyA"
                     /locus_tag="AC06IO_000901"
     CDS             9520..10314
                     /gene="thyA"
                     /locus_tag="AC06IO_000901"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIO9874111.1"
                     /translation="MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAKGFPL
                     VTTKKLHTRSIVHELLWFLRGDTNISYLKENGVTIWDEWADNNGDLGPVYGKQWRSWP
                     TADGRTIDQLSDVVQQIKSNPDSRRLIVSAWNVGELDQMALMPCHALFQFYVANNKLS
                     CQLYQRSADVFLGVPFNIASYSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQT
                     QLTREPLPLPSLAIKRKPASLFEYVYEDFEFVNYQSHPAIKAPIAV"
     gene            10390..10659
                     /locus_tag="AC06IO_000902"
     CDS             10390..10659
                     /locus_tag="AC06IO_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874112.1"
                     /translation="MKKIMVFVLLTVVCSAGFSSKLSKFLNKMDAEQKQRDAQELQQD
                     MNFGDYAFRLKERYVDDRGQRCREYEFRGRSNPYRHGYFTVCDDR"
     gene            complement(10776..11168)
                     /locus_tag="AC06IO_000903"
     CDS             complement(10776..11168)
                     /locus_tag="AC06IO_000903"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIO9874113.1"
                     /translation="MTEINKIIHKKTFDIAWGDMDALGHVNNARYFDYFQEARIDWLR
                     ELDIKMTGQTGPVVIHVACTFLKPIVYPATVTIHSKAHSLGNSSMIMDHDLYQEETLM
                     AQGVSKIVWIDYTQNKSVPLPDIIRNLV"
     gene            complement(11165..13342)
                     /locus_tag="AC06IO_000904"
     CDS             complement(11165..13342)
                     /locus_tag="AC06IO_000904"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIO9874114.1"
                     /translation="MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQTRVGI
                     VINKVCDQQTGHSLKKITSIIDEKPLVPEALLELCLWVGSYYQSPLSEVIPLVIPKKY
                     RLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
                     NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVASIFEHIHHYQCFL
                     LQGVTGSGKTEVYLHVIAKILEQGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSN
                     LNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSA
                     RDTALMRAHLANIPIILGTATPSLETIHNCKQKKYNLLRLTHKAASTTPLHYQLIDLR
                     SQMVQHGLALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLT
                     LHKQAGQMICHHCGLTQRIPLFCNNCQSKELIPVGSGTQRIYEFLSHQFPDTNVLRID
                     RDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGFYNQDFR
                     AIEHLGQLLTQVSGRAGRAEHPGQVLIQTYSPDHPLLNLLIQHGYDEFANALLISRQE
                     AEMPPFQFLAVIRAQGKTVNKVDQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQ
                     LLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVDPMDLS"
     gene            complement(13343..13837)
                     /locus_tag="AC06IO_000905"
     CDS             complement(13343..13837)
                     /locus_tag="AC06IO_000905"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIO9874115.1"
                     /translation="MKKYLTTVELQKESMKFSAGHTTIFSATEREPLHGHMYSVYLAL
                     TTWVEENGMTFDYRYYKERIHKLCRYLNQTFLMPQFSPFLEYSEDEEYYYFIFNKKKI
                     PFLKEDVTLMPLTNITVEELSRWFVNELIKEKEELDKHRIEKVVVKVFSAPGQSASHE
                     WLRS"
     gene            complement(13840..14952)
                     /locus_tag="AC06IO_000906"
     CDS             complement(13840..14952)
                     /locus_tag="AC06IO_000906"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9874116.1"
                     /translation="MNLKQTLINQLKLQKEIPFIDFMQQALYAPYEGYYTSGLQKLGK
                     HGDFITAPELTSLYGKTLANQFQQILPLLDSPVLFEFGAGTGKLCIDTLTHLEQLQCL
                     PESYFILEVSANLRHRQQELIQQKIPQLAGLIHWLDKWPEAPFNGVIIANEVLDAMPV
                     YRFMQSEIEILESYVTLDEHDQLAEIFKPVQNQRLLAYIKNRLPPLDYPYLTEANLFL
                     DDWLLNCSHMLKKGALFIIDYGFPRHEYYHPDRNQGTLMCHYQHYSHPNPLLNPGEQD
                     ITAHVDFTHVAEAGHQAGFHIAGYTNQASFLLANGLLSLIHTLDDAQELFAAKQAIKQ
                     LTQPSEMGELFKVIALTKDLEIDLNGFLINDKRVNL"
     gene            15129..15884
                     /locus_tag="AC06IO_000907"
     CDS             15129..15884
                     /locus_tag="AC06IO_000907"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HIO9874117.1"
                     /translation="MNQTNMQEAKVALVTGGAKRIGAAIVKKLHLSGYQVAIHCRSAL
                     HEADILAKDLNRLRTNSAIVMQKELTEPDAAEEIISKVKHWAGRLDLLVNNASVFKRT
                     DCHLLNKADWQYLFAVNVQAPFLLSMAACPLLAPCKGSIINITDIHADKPLKGYSVYC
                     QTKAALEMQTRSLARELAPEIRVNAVAPGAIAWPENDNSLTAEAQQKIIEKTPLKSHG
                     KPDFIAQAVLALAENSFITGQTLKVDGGRSLTS"
     gene            15941..16300
                     /locus_tag="AC06IO_000908"
     CDS             15941..16300
                     /locus_tag="AC06IO_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874118.1"
                     /translation="MSKWILLSGSLFLCLFSLSVHSNSFDKDQLVQRCQILHEELKEL
                     ESHQYKGICRHKLALAANKIFSAKVRIVYENYKGAKQDLSVSMNNLKFAEDISCVFKS
                     EITKARMEAREIQRELN"
     gene            complement(16359..17345)
                     /locus_tag="AC06IO_000909"
     CDS             complement(16359..17345)
                     /locus_tag="AC06IO_000909"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIO9874119.1"
                     /translation="MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPA
                     YCVSEMCSAIDILHKHSPQSRYIYRAPTEKLLAYQISGNEPHVLAEAAAKLQSLGADL
                     IDINCGCPKSKICKKGAGSALLEDPQKLIAIISKVRAAISIPLTIKIRIQNNEKDLTL
                     AKQIEGAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDISNISSLRKA
                     LEISVCDGFMISRAGTGKPWLYRDLLNLETNDVAFDEKLKLFMLHLEGLSCLENEHKA
                     VLQSKSLVHYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHRVSNILVAAS"
     gene            17427..17870
                     /locus_tag="AC06IO_000910"
     CDS             17427..17870
                     /locus_tag="AC06IO_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874120.1"
                     /translation="MDRRYLSPLELLGIATQHAYTADYMLQQIANGMYRGGETIAVFS
                     PITSLMYVAFQLTFKAYCLHDHRPIKEYKNLMELVELNSHLGLSSNDIFLLKTLSRQQ
                     VFNKGVDYDLWENQQQLHVFCEEIISLYERVQSMMPLELQSDYQE"
     gene            18047..19042
                     /locus_tag="AC06IO_000911"
     CDS             18047..19042
                     /locus_tag="AC06IO_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIO9874121.1"
                     /translation="MEQLDDKVADNVQQKILQLSRHLNSRILGQKDLISRLLIALLAD
                     GHLLVEGAPGLAKTRAVKELSDVVEGDFHRIQFTPDLLPGDLTGTDVYHPQNGSFVFQ
                     PGPIFHHLILADEINRAPAKVQSALLEAMAERQVTIGGKTYPLPELFLVMATQNPIEQ
                     EGTYPLPEAQLDRFLMYVKISYPDAKVEHDILTLARKEALGNATENQVKVTKLSQKTL
                     FEARKQVLKVHTSEALENYLVQLVVATRNPGVYSDELGRWLRFGASPRATIALDRCSK
                     AHAWLAGRDYVTPDDIHVIAHDVLRHRILLSFEAEAEGISSDDFIDSLLRLVAIP"
     gene            19052..19963
                     /locus_tag="AC06IO_000912"
     CDS             19052..19963
                     /locus_tag="AC06IO_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIO9874122.1"
                     /translation="MTDGVVAELSELIDLRRYVHSVHYKPQGRALRSGNHISKFRGRG
                     MDFAEVRNYQAGDEIRHMEWRVTARTGRPHVKIYQEERERPVVLLVDFNPSMIFGTRI
                     AFKSVVAARLASLLAWTVIKQGDRVGGFFFSATEHSEFIPRGRDSGVLPLLASLSRYT
                     TQTEAQREEKPRMLSDALMRLRRVIRPGSILVLISDFYSMDVECEKHLNRLRGHNDIL
                     AYHVCDRIELAPPKPQQYAISDGKQEIILDTGLETVSAAYQLYCQQRIEQLNDQFKRL
                     QIQYVQVTADTNLTQLVRLTFPRRSRG"
     gene            19956..20441
                     /locus_tag="AC06IO_000913"
     CDS             19956..20441
                     /locus_tag="AC06IO_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HIO9874123.1"
                     /translation="MAKTEPLAQLRDIHLPEPVGWWPLAPGWYGLMVLIVVLVAGVAY
                     FIYKRHVNALPKKQALSLLKIHKEQYEKDKNTQLASAHISELLKRVALVYYPRAEVAS
                     MHGEAWVEFLNQTGKGIDFTPVKSMLLDSPYKTSDALNLNPLFTRAEKWIKQRGAPCS
                     N"
     gene            20429..21463
                     /locus_tag="AC06IO_000914"
     CDS             20429..21463
                     /locus_tag="AC06IO_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIO9874124.1"
                     /translation="MFELATPWALSLIPVPLFLWYLLPRAKIKLPAALKVPFFEAMAG
                     IVEQENRLISAKTLLLIPVLVWVLLVIALSGPRWVGEPKPVAREGYNIMMVLDLSGSM
                     EITDMLLHGRPVSRLLVVKRAAEQFVEDRVGDRIGLILFGTRAYLQTPLTYDRHSVLM
                     RIDDATAGLAGKTTSIGDAVGLAVKRLQDVPSKGRVIILLTDGANNSGVLAPLKAAEL
                     AKQDGIKIYTIGLGSEADPRALTGDFFAPTLSAELDEKTLEKMAKMTGGRYFRATDPE
                     SLQSIYQTINQLETVKQEQATVRPQKEYYPWFVAFALLLCFYWLISKADLFVYLRSFL
                     KVERSLSHDS"
     gene            21453..22385
                     /locus_tag="AC06IO_000915"
     CDS             21453..22385
                     /locus_tag="AC06IO_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIO9874125.1"
                     /translation="MIAEFHFLRPWCLLMIVPLLALVVIYWRQKPKLHAWEAVCDPHL
                     LEHLLHSKGQSKRALSLSFLFFSILFMILSVSGPTWYKLPQSTYKPIQPRVLLLDMSD
                     KMMLGDLSPNRLSRAKFKLHDLFTRKDIGQFGLIVFTGEPFVVSPLTDDGQTISSLLS
                     TLTPDIMPVAGYNLDSALDEASQLIKQAGYDHGEILVLTAETPSSSAIEKAKKLAESG
                     IFSSIMPIRADENLNPLFQRFANAGDGYLLVYSPDSSDLEKWLNASQGKEKFALEKNE
                     DIPLWRDEGRWFLIPALLLLLPVFQRGWMQRVTV"
     gene            22382..23263
                     /locus_tag="AC06IO_000916"
     CDS             22382..23263
                     /locus_tag="AC06IO_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIO9874126.1"
                     /translation="MMWRVLTIVAAALISNQLKAFSWSDLWFTPNQQAQHLMEKGQFA
                     KAKDLFERNDWAASAAYRAGDYEQAAKLFNELKTEQGYYNQGNALAHLGKYEEAIRAY
                     DKALAFNPNNQDALYNRELISELLKKDKEKKKSQDQQNQDKQNKDQQNQDQKNQDKQN
                     KDQQNQDQKNQDKQNKNQQNQDQQNQDKQNKNQQNQGQQNQDKQDKNQQNQEQQNQDK
                     QNKDQQNRDQQNKAQQNKNQNKKNDKQEGEIQSESNQEKQLAKEQWLRLIPDDPGGLM
                     REKFLRDHLRRERGWYQ"
     gene            23260..24909
                     /locus_tag="AC06IO_000917"
     CDS             23260..24909
                     /locus_tag="AC06IO_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIO9874127.1"
                     /translation="MKKLTLWVLLSLFSSLSFAEIKVELEPSQVTMGETFKLSITQED
                     QQGGGVPDLTELQKDFVILGTERHVNYSIINGQAQSQSQWIVLLRALKPGIFTIPPIK
                     VGLEQSLPITINVQGSANTQALPDEAIQKQDVFLTAEVNEKNPYVNQQIIYTVKLYNS
                     KRLLDADYQGPKVDDALLIPLGDAKRYHAVHNNINYVVEEQTYAIYPQKSGTLKIISP
                     IFNALVYDYNPQRTKATDKAINLTIKPIPKKYQGKLWLPAKQVKLSEQYENSNQTLSQ
                     GSTLTRTVTLEGIGIPAQLLPTLTFQETDFFSVYPEKGAERNQVKQGELIGSVEIKVT
                     YLFNKAGKTTIPELRLPWFNTETGKEEIAVLPPRSIDIIPSTAVKANTANTQVTGKEM
                     SASSQSVPDSASVSNESGGWAVKLAIFFGLAWLITLSLWIWQKRPRESSSKKEYKKAL
                     DALNKACSQCNPKKARDAVLKWASLQWPDAPMLNLTDLAQLVRDAHLRKQLHLLSQVL
                     YRSQEKTLWRGDELLRAVQAVKRNESVKKRKNSKLPPINPL"
     gene            25146..26528
                     /locus_tag="AC06IO_000918"
     CDS             25146..26528
                     /locus_tag="AC06IO_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIO9874128.1"
                     /translation="MHVNEYIHYDVQELAELIKNHQVGAEEVLRCAQERLYQVNPSLN
                     AVVTDCFEYAHQCLAKMKGNEPYYGVPLLVKDLGHAIEGIRLTEGSRFFSKNLAETNS
                     DLINKLIALGFVPFAKTNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAAIM
                     AGIAPVATASDGGGSIRIPAACCGLFGFKPTPGLVPSGPWVDEAWSGMATSFILTRSM
                     SDAIALFKNVINPMRLNRLSRKKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNS
                     GHRLNEVHLPLDLQVISSCAHTLIAASTYAKVKTQEQISGRKPMIHELEPITWEFYYR
                     GKMVSAYEFIIAKNKLYQSLRPLHQLLEKTDIVLTPALAQLPLLIGHLRIDEEFNKYL
                     QKKKEFSPFTSLFNQGGLPAMTVPVMFHHHLPVSVQMGSAKGNDLLLLELAHELQSVL
                     PKNITPASIP"
     gene            26809..28266
                     /locus_tag="AC06IO_000919"
     CDS             26809..28266
                     /locus_tag="AC06IO_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9874129.1"
                     /translation="MKTLPSKVIFIGAGPSGLFAARQLKEIAKLQNKTVECIFLEREP
                     VVGGKCHTYSDAAHPELKTEWGAALVAPNYGVVLDALAEHGIEFEKVMPTASETIEIK
                     QLFEKASSLEKISMEMKLLGELWDFNSDYDVYKKAKQNKKALPDKLLNPFSEYCKLNN
                     MSYLPVLLKPFVPGFGYGDLNFCPAYCVLEYMGKVTMPDILLADQLIGRPSLLAIHGG
                     FQLLMERIAEQFDVRTSASVSRIKREKNKVTVTYVHNGIEQTETADTLVLATSPKNWK
                     SLGLDLTEVENNCIESLDYYRYPVAVYKIKGLPPQQYFFTKALEERGFGHLALITTRD
                     NRSNPEDGRLCTVYVNLSPNNNEFKFDHEALRKELESIPDVTEVTVVKDKIWEDYMST
                     LPWDLRLQLDKEQQRTNTMYLGSYALGSFEDVACVANKATDAMTEWYAPTMRYEEDFS
                     WKNMNRAWQFFTSPTKSPLASLGEPRMSDEHSNRP"
     gene            28505..28720
                     /locus_tag="AC06IO_000920"
     CDS             28505..28720
                     /locus_tag="AC06IO_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIO9874130.1"
                     /translation="MSVKETGHVKWFNDDKGYGFISRDNGQDDVFVHFRSIVSSAGRK
                     TLLEGQRVEFLVTKGAKGLQAEEVTAI"
     gene            complement(28820..30124)
                     /locus_tag="AC06IO_000921"
     CDS             complement(28820..30124)
                     /locus_tag="AC06IO_000921"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9874131.1"
                     /translation="MTQQDPVVIKSKGDQALEKGDLPLALTLYDEALAINPQFSGAYY
                     QKGTVLLRMGKTIQAAAMYWQAYLFSEFKTDIGLMTAKTLAHANYSLSACKLFESFKI
                     EEMDGESLAYYLYALRQQGRVQEAYSLFPYVNQFNIPKTSWARAIILLDLNKVEEARF
                     LLEPLEKTDKDGHITILLHAVYLALNDKKAVKSLLDRAIQRIPNNDYFCCQRIAIDIL
                     NGLNKTPIETYRAFKRFDLIDAADYLHKHADKHLRHSGTTYQTFDLLAPQVLSEGLIL
                     EFGVRFGYSISHIAKLFPKRKIFGFDSFQGLPEDWHHEKAGSYSTYGKIPSVASNVTL
                     IAGWFNETLPDFKKNNREPIAFMNIDCDLYSSTKLVFNELNPQIAPGTIIVFDEYIGN
                     INWRQDEFKAFQEWVKENKVEYRYLTASFYTKQVSVQILKRK"
     gene            30288..31268
                     /locus_tag="AC06IO_000922"
     CDS             30288..31268
                     /locus_tag="AC06IO_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033897 - ribonuclease T2 activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2 family protein"
                     /protein_id="HIO9874132.1"
                     /translation="MIKPITLLLLFCSFTVNASILVNGTFEATQSCPAYLSKNRKSNP
                     DNLMVEPNQKYQLKEINKTKPDWLRIQFSDRQPNLRWVSVDCGVIEYTERPKASCDLS
                     PGMADSYVLALSSQPGFCETYGYEIGKPECLKLTKTAYQANHLILHGLWPNQEACGHN
                     YGYCGISPRTNHCDYSPLDLSSDVAQNLKKLMPSYNYGSCLERHEWNKHGSCQDLSTD
                     GYFSLAMRLTTEVNNTEFGQYLTSHRGDTVKLATLRELIERSFGENNTGKVTLSCKNG
                     ILVDIYVQLPALIPFKESLESLVDKASHSQHHDYCNSNVTISDFSNEKWL"
     gene            31407..32369
                     /locus_tag="AC06IO_000923"
     CDS             31407..32369
                     /locus_tag="AC06IO_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIN domain-containing protein"
                     /protein_id="HIO9874133.1"
                     /translation="MLKRCYLLILLMFVLASCAHHKTQTPPAEVKKQSTSSTRQFRQV
                     SSFNQIVVQGRLNVNLHTGYNKPEVMLRGDPRDLVQVRTVVKQNTLYVSLGQGYPDYG
                     TVTVDIKTKFLNRFRYEGAGVVTGNNLRTSYLDLYLANEGTTRLAGNIGLQKLEAVGN
                     GVTQINGVSSRNLQIVLKGDPKVLISGFVNLRQLDMYGKGTLSLYWIKSDTLTIRAKK
                     AAKIQLAGIVNRLDVELWDFAQFKGKYLRAQRSFVKTHDKSVAEISAVNHQSSLATDA
                     SDIYYYNLSKTRADFMAFNGSVLDMREWSQSDLKDFDRYNKQFP"
     gene            complement(32436..32669)
                     /locus_tag="AC06IO_000924"
     CDS             complement(32436..32669)
                     /locus_tag="AC06IO_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874134.1"
                     /translation="MFFYNASLKNDELNASKIAILVTGLASLLLIGNYSDEESYEDVS
                     PSFLSQLMNDPSNDPFTSDEEIFEVNIGLPFRL"
BASE COUNT         9814 a         5947 c         7152 g         9882 t
ORIGIN      
        1 tatatttttt gaaggaatga gacatacact aatagattaa gtaaggtaag tggggctccc
       61 acaaatataa actccagaaa tccaaagcct ctgataccac gtttttcact attttgaata
      121 ataataatgt tacttgctgc cccaaggata gagagattac cggcaatagt acttcccgca
      181 gccaggctca gcagggttat attggaatga ttatgactca tcaataaagg caaatatatt
      241 gttactaatg ggacattaga gatcaattgg ctcagggcga tactcattat aaaaatcacc
      301 acgatgtggc taacatcaat atgagaatca ctcattccgg tttggaaaaa gccgctatcc
      361 catacgcttt gcattaaaat aaaggtgctc gcaaaaaaaa ctaaagttcc ccaatccagt
      421 ctttttatat aattccatcg atgcttgcta aaccaaattg gtacagacga caagagtgca
      481 atataactaa aattgagctg aaattctgaa taatgcagga aatccgtcaa gattttgatc
      541 atagtcagaa ctaacatcat gagcaagctc attttaacta aagtaagatt actacggtca
      601 tttatcaaag ggagaactgg ttttttaata ggttctttta attcatttct gtataaaaaa
      661 agaataaaaa aataaactat acccagattt aacaaagtag gtacagccaa gtgtttgaaa
      721 aactcaaaaa atggcgaggc catctctccc ttaatagcaa tcaataaatt ttgtggattg
      781 cctataggac tcatggtgct tccaatagta attgaaaatg ccaatgccaa cagcaagggt
      841 ttaattaaat ttttatgtga ttgacacagt tgtaaaatga taggtgtacc tataatggca
      901 atggtatcat tcattaacac cgcggagctt aaacccagaa taaaaacaat caacattaac
      961 gtttgttttc cattattcgc ataataaaaa atttgatcgg tcagaaaagt gaggtagcca
     1021 ctttcctctg cagcttgtga aatgaaaaaa acaccaaaca aataaagcat gacatctggc
     1081 tccacggcag cgaaagcgcg tgatggcgtt atctgttggc agagcaaaac agcaatggct
     1141 cctgttgcca taattgccca aattggaatg ataactctga caacctgccg taaagcaatt
     1201 gcaataagaa taatcagaag aatgatgaca gtagaaggca tttctgtcct cttttattta
     1261 atccctctcc ctttttaccg gccttttcca gccgtagatg gttttttgtc gtgattcggt
     1321 taatgttaac tcatcgggcg gcacattccg gcgtatggtg ctccctgctc ctattgttgc
     1381 gttagcgccc acggtaactg gagcaactaa ttgagtatct gacccaataa aaacaccatc
     1441 ttcgataatt gtttgatgct tattaacccc atcgtaatta caagtaatgg ttccagcgcc
     1501 aacgtttaca ttttttccta acaggacatc ccctaagtaa ctgagatgac tggctttcgt
     1561 gccttcatca aaaatcgctt ttttagtttc aacaaaatta ccgattttac aatggggagc
     1621 taattgcgtc cctgagcgca agcgggcaaa aggaccgatg tggcagtcgt ttgcaatgtg
     1681 gcagccttcc agtacgctat tagcgtaaac ttcacatcca tcgcctaatg tgacattagt
     1741 cagactgcag tttgggccaa ttttacagcc attacctaaa actaccttgc ctgtaaaaat
     1801 gcagttaatg tcgatataga cgtctttgcc acaatataat tttcctctca aatcaaagcg
     1861 gttggcatca gccagggtca cgcctttttc catgagttga ttggctgctc tttgctgcca
     1921 gatgcgctca agttgctgaa gttgttggcg attattaatg ccctgcacct caaagctgtt
     1981 cttggcggtt aatgacgtaa tgggtgtttt attttgtaca gccaatgaga taatttcagt
     2041 taaataatat tcgccttgtg cgttactatt acttagttga ggcaaccatt tcttcagatt
     2101 atttgccaaa gtgcaacaca ccccggaata aatttctttt atattcttta cttgctcatt
     2161 tgcatccttt tcttctacaa tgctataaat ttcaccttgg ttgtttctga taatacgccc
     2221 aagaccgctt ggattttcca gttctgcaac caggagtgct aaaacagaat gatctggatt
     2281 ttgacgttgg ctgcattcaa ttaatgattg aagagtttcc acttgaatca ggggaacatc
     2341 ggcagataaa accagaacgt atgcatcgtc agggatatgt ggcatggctt gcaaaacagc
     2401 atggccagtc cctagttgct ctgcctgatg cacccaatga acaggtaagt tggggagtga
     2461 acttttaatt tgttcaccac catgaccata aatgacatga attgcatcgg gatttagttg
     2521 ctgagcggtt tccacgacgt gggttaacag cggtttgcca gccaggtggt gaagaacttt
     2581 gggcgtgtcc gaatacattc ttttaccttg ccctgcagct aaaataataa tttgtaaatt
     2641 catgagattt gtgttttcct aagttaaata tcataatcgg tgctgattta aacagtctgc
     2701 cccaatgtta tgaatgatgt aaagagctat atgataaata agttgcagtt aaaggagtag
     2761 agcaatgaat atatttctaa aggttttaaa gaaattaatg aaggaaaaat tgagtgagtt
     2821 atccgccaat ttgaaatgga agtcagaaac cattgggtat aataccaaat tatcaatcga
     2881 tcaaaagaat gtcattgata aactcgaaga aagtatcgtt ctttttaaag actcaggtaa
     2941 tgacgaagca gatttgctgt caatcaatgc attgatagat gctgccagaa tacaaattca
     3001 atccataaga gaatcctatg gtgagccgag agataaaggg gaatctgtaa cctgtctcaa
     3061 taatttgaaa aatcattcca acgattttct tggcaaacta aagaaatttg attttgacct
     3121 gttaaataaa gcttatacag aaacacctga aaatattgtc tattaccatg ccgctatcta
     3181 tctgggagac gaaattttta gccctagaac agggatagat tacgaaatcc gcactaaaaa
     3241 agaagtgcaa ttggcagtaa ggctgcaggc attaagcgag cgaattaatc caagccacaa
     3301 tttggaggaa caaagaaaaa gagccctgca agtgttgcaa gatcttgctc aggacaatca
     3361 aaatgccatc aagaaagata aaggtttttc tcttcctggt ttaagctttt ggggtgtttc
     3421 agtagttaca ccatcggatt ggtttagctc aggagaaggg cggtttggcg ttgaatttaa
     3481 tacggcagtt cgcaccattc agggaatgac tgaaagccag tttgaaagac cgacagtaaa
     3541 acaaacaacg gcaaagcaag aagagaccaa accatccgat gtaaaaaaaa cagaggcaac
     3601 agacgaggag gatgaagaac attcatctga aaatactcat acattgtaag ttactttcaa
     3661 tgggtgtatt ttgatttctc tgcctcgaat gcatacgaga ggtctccaaa agatatattc
     3721 tgttggagca aggagatccc tcgttcttaa atctgcagtt caccacacaa attggtttca
     3781 atcagttttt tgatttcagg gatttccaga ttcttgctta ataaatacag caatttgcaa
     3841 tgagccgcct caggtgtcat atcatgaccg ctaatcaagc ctgcttcttt taaagtatgt
     3901 cctgttgcgt attgattcat ttctactttc ccctggtggc attgggtgca attaataata
     3961 ataacccccc tgccgcaagc ctccttaagc aaacttaaaa atcttggatc attgttctgg
     4021 gcattacctg ccccataggt ttcaaggatt aaaccccgca aaggttgatt taaaatgtac
     4081 tcaagaacat cagaggcaaa gcctgggaac agacggaagt ttgcgataaa gtgaggagta
     4141 atggtttgta gatggaacgg tgctttggga cgtggcaata aacggtgttg atgtaactcg
     4201 atatcgatac caatcgatgc caagggtggg tagttgggcg aatcaaaagc attgaagcgt
     4261 tgggcactga ttttttgagt gcgattgcct cgaaacaaat gttgattgaa gtagatgcat
     4321 acttcatgga taggttgatg agcacaaagc cataaggacg taatgagatt atctgtcgca
     4381 tcgttacgaa tctcggataa aggaatctgt gaaccagtga ctatgacggg ttttcccaaa
     4441 tactccaaca taaatgacaa agcggaagca gtgtaagcca tggtatcagt gccatgtaat
     4501 acaacaaatc cgtcaaactg ttcatattcg tgagcaatat ctctggcaat cctgttccat
     4561 tccgtgacgg tcatattaga ggaatcaagt agcggctgat attctttgat aacgtagtca
     4621 ggcatgtctt gatgttttag agcaggaatg tctgccaacg ctttttgaat gaatcctggt
     4681 gaaggctcat aaccatgcga tgtttttatg ctactgattg tgccgccagt attgataatt
     4741 aaaactcgtt ttttcatgcg gatttataat caatgattta ttattgtttc gataagtata
     4801 gcgaaaactt ggctatacca ataacaacta atttcaagac ttgcgaaaag ccccaatctc
     4861 tttggtaaaa aagtgtttaa ttcggtcatt tagtttatag aaattccctc gttaaaaaca
     4921 ttaaagttac ctgtctccaa ggtattgaga gtcattataa tgagtaattt ttgttctata
     4981 ataaatcaaa aaatgcaata taaaaacagg gaataaatta gtccaaataa tatttacaga
     5041 tttaattgta agctcttgta ctttgtggaa aaatcaagtt attaacggtt tattctaagc
     5101 agtgacatca tggttatcga tcgtgccgga tatcgcttga atgtaggtat tatactcgtc
     5161 aacgattccg atagagtttt ttgggggaga cgaagcgggc atgacgcttg gcaattcccg
     5221 caaggtggat tggcaccagg cgagacggcc atgcaagcaa tgtatcgtga attgcatgaa
     5281 gaagtggggt tagataaagg tgatgttgaa atattgggtt cgaccaggcg ttggctaaag
     5341 tatcgacttc cgaagcaata tttgcgtcat ggcagtgagc ctttggtgat tggccagaaa
     5401 caaaaatggt atttgttaaa gcttgtaaca agcgagcaaa aagtcagatt ggatttgagt
     5461 gattctcccg aattcgatag ctggcggtgg gtggattttc atgagcctga gcaacaagtt
     5521 attttcttta agaggcaggt ttatattcaa gcgttaaaag aattagaacc tttgttaaaa
     5581 aaagaacgcc gtacccctta tggcttaaaa cgtaaaagag gtaatcaaag agcttagatg
     5641 cttaaaatac ttaaacggat agtacaagat gttaccgctg ccagtgattt gaaagaagca
     5701 cttggcattt tggtgcaacg tattaataca gccattaatg cagaggctgt ttccgtttat
     5761 cttattgata ataaaaatgc tgaatacgtc ttgatagcca cggaaggttt gaacaagcag
     5821 gcagagttta gagtcagaat tgctcttgat tgtggcctta tcgggttgat aggacgaaga
     5881 gaggaaccta tcaatataga aaatgctccc tctcatccag acttttatca taatccatta
     5941 attggtgaag atcacctgaa cgctttttta ggcgtcccta ttattcaaca tagaaaatta
     6001 tacggtgttt tagttgtaca acagcttgaa cagcgtcatt ttgatgatgc tgaagaatca
     6061 tttcttatta ccttggcggc ccaattaggc gggattattg ctcatgcaga ggcaacaggt
     6121 gaattggccg agttaacaca acctaaaaag ggtataggag tcgacaaggt agaagcggct
     6181 tatactgcac tgactggaat aggcagtgtt cctggtatag gaattggtac tgccgtggtt
     6241 gtgtacccgc ctgcggatat tgatgctgtc cctcgtaatc cggttgagga attggaagat
     6301 gaaatcaatg cgttttatga agctttggaa acgacaagag atgatatcag gcgtttaagt
     6361 aaacgcatga aagaaactgt tgctgaggaa gagcatgctt tattcgatgt gtatttaaga
     6421 attttggata gggatagctt gggtgctgaa gtagagcatg tgattcgtga agagaaaatc
     6481 agcgctcaag ctgccctggc tacagtgatc aagaaacacg tgcagcaatt tgaaagcatg
     6541 ggcgatgatt atctgcgcga acgagccagc gatttccgtg atttgggccg aagagttttg
     6601 gcggaattac aacgtaccca aagagaagaa attgtttatc cgaagagaac cattttaata
     6661 ggagaagaaa ttactgctgc cgctctggca gaagttccag aagggcaatt ggctggtgtc
     6721 gtttcagcta aaggctccaa taactcccac gttgctattt tagcgagagc tctcggtgta
     6781 cctactgtga tggggttgag aggattaaaa gtagaattgg tatcgcgtcg ggctttgatt
     6841 gttgatggct attatgggca tgtttatatc tctccttcca aatcattatt agcggaattt
     6901 aagcaattag ctcaagaaga acaggaatta aaccaaagcc tggtaagctt gcgtgataaa
     6961 ccagctgaaa ctactgataa ctacagagtc tctttgcaag tcaataccgg attggcaatg
     7021 gatgccggtt tatcaatgag tgttggcgct gaaggagttg gtttataccg ttcagaagta
     7081 ccatttatga gtcgagatcg attcccttca gaagatgagc agaccattat ttacagacag
     7141 actctcaagg cctttgctcc acgtttggta actatgcgta ccctggatat tggtggagac
     7201 aaaattctac cttattttcc agttgaagag gaaaaccctt atttaggatg gcgtggaata
     7261 aggataactc ttgatcatcc agatgtcttc ctgattcagg ttcgtgccat gatgcgtgct
     7321 agtgaggaac tggataattt acgtatcatg ttgcctatgg tcactacgct tagcgaagta
     7381 gaggaagccg tttatttgat tgaacaggca tttgaagagc tggtggaaga ggggtgcgaa
     7441 atagtaaaac ccaaaattgg tgtgatgatt gaagtgcctg ccgctgctta tctggcaaga
     7501 gaaatggcga aacgagtaga ttttatttcg gtgggtagta acgatcttac tcaatattta
     7561 ttggctgtgg atagaaataa cgcccgagtt gccgcacttt atgatccctt gcatccagcc
     7621 atgttgcgta cattgctaaa aatagtcgaa ggagggcatg cagctggtgt tgaggtgagt
     7681 atttgtggcg aaatggccag cgatccttta gcggtgatct tgttaattgc tatgggtttt
     7741 gacaccttaa gcatgaattc cgccagttta cccagggtga aatgggttat tcgtaatttt
     7801 gccattgcaa atgcccgtaa aattctggca gaagtcttag agtttgaaca tcctgctgaa
     7861 atacgatttc atttgcaaaa ggctctagaa gaggaaggat tagggggatt aatcagagca
     7921 ggaaagtcat gatatccggt gagttactga ttaatggagt gatttacatc attattggtg
     7981 cttttgcagg aataatggcg ggtgctctgg gcgttggtgg cgggcttatt gtggtaccag
     8041 gactcgtcta tatttttcaa cactcgcagg tgattccaga agatatcctt atgcatgtcg
     8101 ctgcaggttg ttcactggcg gtgatgattt ttacttctta tgcatcctta aaagcacata
     8161 ataaaatgga tgagatactc ttaccccttt ttaagaagtt atgtccgggt ttggttcttg
     8221 gtactatttt aggttccata atggcttctt ttattccaac ccatttattg aaaataatat
     8281 ttggactgtt cttgcttggc gtagctatga aaatgcttct tgatgtccga gtgagccaca
     8341 ctgataaatt tccgggatat tggctcaata ctttagtcag tagttcaata ggttcgatat
     8401 ccggtttatt aggggttgga ggaggagtca tcattatccc ttatctgacc tattgtggag
     8461 ttccagtcag aaaaattgct gcagtatcca atttaggcac ttttgcagtt ggtctagtgg
     8521 gtacaattat gtttatggtt actggcacat tagacatgac aaaaatatcg tattccacag
     8581 gatatatata ttggccagca gttttagggg tagcaattcc aagtagtttg attgcaccat
     8641 ttggggcgaa attaaattat cttttacccg taaattattt aagatatgga tttatcgtga
     8701 ttttaattac caccgcaatt catatgtttt tgtgaaagga atttgaataa ccatgctcac
     8761 ctatccaaat attaatccaa ttgctttttc tttagggcca ttaaaagttc attggtatgg
     8821 tttgatgtat ttaatcggtt ttgttggcgc ctggctactg ggttattggc gcatcaaaca
     8881 ttataaattg aactggaata acgaccagtt aagtgatttg atcttttata gtgcccttgg
     8941 tgttattctt gggggacgag taggttacat gttgttttat gactttcagg agtttatcca
     9001 ccacccctgg gtattattta aaatatggga aggagggatg tctttccatg gtggtttgct
     9061 tggagtggta attgccgcct ggctcttttg ccgtaaatac ggaaaaactt tcttggaggt
     9121 tggagacttt gtcgcccctc tagtcccttt aggtttagcg gcaggtcgcc tgggaaattt
     9181 cattaatggg gaattatggg ggcgtgtcac tgatgttccg tggggtatga tatatcctca
     9241 tgtcgatgat caaccaagac acccctctca atggtatgaa tttggtttgg aaggggtcgc
     9301 tttatttatt ttgatttggt gttatgcgtc aaagccgaga caacaaggga gagtgagcgc
     9361 tttattttta atgggatacg cgatttgcag gttaattgct gaaagcttta ggcaacctga
     9421 ttcgcaatta ggttttgtgg catttggctg gctgacaatg gggcaggttt tatccatccc
     9481 tatgttatta attggaattt ggttatggtg ggctaagcga tgaaaacata tttgcaattg
     9541 ttggagcata ttttacaaca tggggtagaa aaatcggata gaacgggcac tggaacatta
     9601 tctgtatttg ggtatcaaat gcgttttgat ttggccaagg gattcccgct tgttaccact
     9661 aaaaaactac acacgagaag tattgttcat gaattactat ggtttttgcg tggtgatacc
     9721 aatatttctt atcttaagga aaacggggta actatttggg atgaatgggc agataataat
     9781 ggtgatttgg ggcctgtcta tggaaagcaa tggagaagtt ggcccactgc tgatggacgc
     9841 accattgatc aattaagtga tgtagtgcaa caaataaaga gcaaccctga ctctcgtcgc
     9901 ttgattgtaa gcgcctggaa tgtgggtgaa ttggatcaaa tggctttaat gccttgtcat
     9961 gccttgtttc agttttatgt tgcaaacaac aagttatcct gtcaattgta tcaacgctca
    10021 gccgatgtat ttttaggagt tcctttcaat atcgcttctt attcgttgct tactcatatg
    10081 gtcgcccaac aatgtaattt ggatgtggcc gagttcatat ggaccggggg agattgtcat
    10141 ttgtatctta atcatttgga gcaagcgcaa actcaattaa caagagaacc tttgccattg
    10201 ccctccttag ctattaagag aaaaccggct tctttatttg aatatgttta tgaggatttc
    10261 gaatttgtga actatcaatc acatcctgca attaaagcgc ctatcgccgt ttaattttca
    10321 tcggaattgg atttttattg ccacttgtga tttaatattg cctcaagtgt ttattagagg
    10381 ttcaattccg tgaaaaaaat tatggtgttt gtattactga cggttgtttg tagcgcaggc
    10441 ttttccagta aattgagtaa atttctgaat aaaatggatg ctgagcaaaa acaaagagat
    10501 gctcaggaat tgcaacagga tatgaacttt ggggattatg cttttcgttt gaaagagcgt
    10561 tatgttgacg atcgcggtca gcgttgtcgt gagtacgagt tcagaggacg ctccaacccc
    10621 tatcgacatg gttattttac tgtgtgtgat gatcgttgat ttggctgaaa atactctttc
    10681 tgtatattaa attatcgtct gctccttatg gccgcagctc ggattataac taaaactgag
    10741 ccgcgatcgt aagggagcga ttgtccgaat aggctctaca ctaaattcct aataatatca
    10801 ggcaaaggca cggatttatt ttgggtataa tcaatccaaa ctattttact caccccttgt
    10861 gccataagcg tttcttcctg atacaaatca tgatccataa tcatgctgga attgccaaga
    10921 ctatgtgcct tgctgtgtat ggttacagtg gcaggataaa cgattggttt cagaaaggta
    10981 caagccacat ggatgacaac aggacctgtt tgacctgtca ttttgatgtc aagctcccgg
    11041 agccaatcaa tccgggcttc ttgaaaatag tcaaaatagc gagcattgtt aacatgtcct
    11101 aatgcatcca tatcgcccca agcgatatca aaagtttttt tatgtattat tttgtttatt
    11161 tcagtcatga taagtccata ggatcaacat ccacattcca gcggatgcca ttacttaatt
    11221 tgttcattgt taaccactct cttaattgcg tcaatgaact ttttagaact tttcttgaag
    11281 gggatttaat taatagttgc atcctatgct ggttagcttt tcgtggcaaa ggagctggtg
    11341 ccggacccag taccgtgaga ggatgagcct gaatttgatc tttggttgct tttaggaatt
    11401 gatccacctt gttgacggtt ttgccctgag ctctgatgac cgctaaaaac tgaaaagggg
    11461 gcatttcagc ctcttgtctt gaaattaata aagcgtttgc aaactcatca tacccatgct
    11521 gaatgagtag attaagcagg ggatgatcag gtgaataagt ttgaatcaat acttgcccgg
    11581 gatgttctgc tcgcccagct cgtccagata cttgagttaa taattgcccc aaatgctcaa
    11641 tagcccggaa atcctggttg taaaagcctg catcagcatc tacgacaacg actagggata
    11701 ggcgggggaa atggtgtccc ttggctagca tttgagtgcc tatgatcagc tgagcttctc
    11761 ctttattaat tttgtccaaa tggctgttta gagaattttt tttacgaacc tcatctcggt
    11821 caatgcgtaa aacattcgta tctggaaact gatggcttaa aaactcataa attctctggg
    11881 tgccagatcc tacggggatt agttccttgc tttggcagtt attgcaaaac aagggtattc
    11941 tttgggttaa tccacaatga tgacaaatca tttgtcctgc ctgtttatgc aaggttaaat
    12001 ggctatcgca tgctttgcaa tcaaccatcc atccacattg atggcaaagt aacactgggg
    12061 caaagccgcg tcggttaatg aaaacaagga cctggttttg ttgcagcaaa tgattttcta
    12121 tgattttaag ggttgggaga gccaagccat gctgtaccat ttgcgatcgt aaatcaatca
    12181 gttgatagtg taatggtgta gtggaggcag ctttatgcgt taagcgtaat aaattatatt
    12241 ttttttgttt gcagttgtgg atggtttcca gactgggagt ggctgtacca agaatgatag
    12301 gaatattggc aagatgagca cgcattaaag cagtatctcg cgcagaataa cgcacccctt
    12361 ccatttgttt gagcgaactg tcatgctctt catcgatgat aattaaaccg agattgggca
    12421 gaggggtaaa aacggcagaa cgagttccaa tcaccatttt aactttgttc tcttttgcca
    12481 gctgccaggc aatttgtctt tctgattcat tcaaattgga atgtaatacg gttatgggat
    12541 ggttaaatcg ggctgtaaaa cgagataaaa gctgaggggt taacccgatt tctggtacca
    12601 gcaccaatac ttgtttgcct tgctccaata ttttggcgat gacatgcaaa taaacctctg
    12661 tcttaccact tccggtaaca ccttgcagta aaaagcattg gtagtgatgg atgtgttcga
    12721 aaatggaagc tacggcaacg gcttgttcag gattgagtgt tagcggtgga ctgagttttt
    12781 caggaatttt ggttggcaag gtaacttgct gtgacaggtt aatggcttga caagcaagta
    12841 gtgccgcaat ttgagttccg ttgtagccat gttgttttag ggtctgtttg gaaatccctt
    12901 tctggtgcct gcttaaaaaa tcaatcaact catgttgttt ttttgctttt ttcgagatga
    12961 gtgtctttgc cttttcaaca ggtatattca attgataaaa atcttctgta ggtagtgagc
    13021 agggtaaacc caggcgatat tttttgggta ttaccaaagg aatcacttca gataatggtg
    13081 actgataata gcttcctacc catagacaaa gctctaagag agcttcagga acgagaggtt
    13141 tctcatcgat tattgaagtg attttcttta acgaatggcc cgtttgctgg tcacaaactt
    13201 tattgattac aatgcccact cgggtttgat tgcggaaagg aacccatact cttccgccaa
    13261 tgcatggagt taaatccagg gcttcgtaat caaaacaatc tcgattggta tgggggatac
    13321 aaacttgata aatatcgttc atttaacttc taagccattc atgactcgct gattggccgg
    13381 gcgcggaaaa cactttgact actacttttt cgatacgatg tttatctaac tcctcttttt
    13441 ctttaatcag ttcattaaca aaccagcgtg ataactcttc cacggtaata ttggttaagg
    13501 gcattaaggt gacatcttct ttaagaaaag ggattttttt cttattgaag atgaaataat
    13561 aatattcttc gtcttccgag tattctaaaa aaggtgaaaa ttgtggcata agaaaagttt
    13621 gatttaagta gcggcataat ttatgaatcc gctctttgta ataacggtaa tcaaaagtca
    13681 taccattttc ttctacccag gtagttagag ccaaataaac agaatacata tgaccatgca
    13741 aaggctctct ttctgttgcc gaaaagatgg tggtatgccc ggcagaaaac ttcatggatt
    13801 ctttctgtaa ttccacagta gtcagatatt ttttcataat tataaattca ctctcttatc
    13861 atttatcaga aaaccattta aatcaatttc cagatctttg gttaacgcaa tgactttgaa
    13921 tagctctccc atttcactcg gttgagttaa ctgtttaatg gcctgttttg ctgcaaacag
    13981 ttcctgtgca tcatcaagag tatggattaa gcttagcaaa ccgttcgcca gcaaaaaaga
    14041 ggcttgatta gtataccctg caatatgaaa tcccgcctgg tgccctgcct cagcaacatg
    14101 agtaaaatca acatgtgcgg tgatatcttg ttctcccggg ttaagtaaag gattgggatg
    14161 agaataatgt tgataatgac acattaacgt gccttgatta cgatcagggt gatagtattc
    14221 atggcgaggg aagccataat caataataaa caaagcccct tttttcaaca tatgactgca
    14281 attcaacagc caatcatcaa gaaacaaatt agcctcagta aggtaaggat aatccagtgg
    14341 tggtaaacga tttttgatat aggccaatag tcgttgattt tgtactggtt tgaagatttc
    14401 tgctaattga tcatgttcat ccagagtgac atagctttcc aggatttcta tttcagattg
    14461 cataaagcga taaactggca tggcatccag tacttcattc gctattatca ctccattaaa
    14521 aggagcctct ggccatttgt ccagccagtg aatcaaaccg gccaattgcg ggattttttg
    14581 ttgaatcaat tcttgctgac gatggcgtaa gttagcactt acttccagga tgaaatacga
    14641 ttcaggcaag cattgtaatt gttccaaatg agttaatgta tcaatacaga gctttccagt
    14701 tccggcccca aactcgaata gtacggggga gtccagcaag ggaagaattt gttggaattg
    14761 gttggctaaa gttttgccat acaaggaggt taattcagga gcagtgataa aatcaccgtg
    14821 ttttcctaat ttttgcagtc ctgaagtata atagccttca tagggggcat agagtgcttg
    14881 ctgcataaag tctataaaag ggatttcttt ttgcagcttt aactggttta ttagagtttg
    14941 ttttaaattc ataatcactt aaactatcgt tagacttctt ttaaactctc tgtgaacaag
    15001 agggtgcaag gagaaacaaa gaaaatactg aagtgtacac aataatactt gaggattcga
    15061 gcatcctggc tgattacttt ttgcagtgag gttgcgaaag gagtctgtta ccatatgggg
    15121 aacgtacctt gaatcagaca aacatgcaag aagccaaggt tgctttagtg actggaggcg
    15181 ctaaacgaat cggcgcagca attgttaaaa aattacatct ctctggatat caggtagcca
    15241 tccattgtcg ttctgctttg catgaagccg atattctggc caaagatttg aatcgcttgc
    15301 gtacgaatag cgccattgtc atgcaaaaag aattaactga gcctgatgca gcagaagaga
    15361 tcatatccaa agtgaaacat tgggctggtc gtttggattt gctagtgaat aatgcttctg
    15421 tttttaaacg aacggattgc catttattga ataaggcaga ctggcaatat ttgtttgcag
    15481 tgaatgtcca ggctcccttt ctactgagta tggcagcttg tcctttactg gctccatgca
    15541 agggttctat tattaacata actgatattc atgcagataa gccacttaaa ggatattcgg
    15601 tatattgtca aactaaagcc gcattggaga tgcaaacgag gtcgcttgct cgtgagctgg
    15661 caccagaaat aagagtcaat gccgttgcgc ctggtgccat tgcctggcct gagaatgaca
    15721 atagtttaac tgcggaagca caacaaaaaa ttatcgagaa aacaccgtta aaaagccatg
    15781 gcaaacccga ttttatcgct caggctgttc ttgctctggc tgaaaattca tttatcactg
    15841 gtcaaacatt gaaagtagat ggaggtagaa gtttgacaag ctagagttgt atactggatt
    15901 tcattgcaaa caatcaattt actgtatagg gattactatt atgtcaaaat ggattttatt
    15961 atctggctcc ttatttttat gcttattctc tttaagtgtt cactccaatt cttttgataa
    16021 agatcagtta gtgcaaagat gccagattct tcatgaggaa ttaaaggagt tggaatctca
    16081 ccaatacaag gggatatgtc ggcataaact ggcattagct gcaaataaga tattctctgc
    16141 aaaagttaga attgtttatg agaattacaa aggcgctaaa caagatcttt cagtatcaat
    16201 gaataatttg aaatttgcag aagatatttc ttgtgttttt aagtcagaga ttaccaaggc
    16261 cagaatggaa gcccgagaaa ttcagcgtga gctgaactaa atagcggaat gtaatggtta
    16321 tcaaattata gaaataatat acaagtcctg accttatatc atgaggcggc aactaatatg
    16381 ttggaaactc tgtgagttgc ctcagctaaa aaatccttga tttccattaa tgaacctaat
    16441 tgatagaact gttgcagttg ttgttcattc agttgctttt tgaagtaata gtggaccaga
    16501 gacttgcttt gcagtaccgc tttatgctcg ttttcaaggc atgacaatcc ttcgaggtgg
    16561 agcataaaca gttttaattt ctcgtcaaaa gcgacatcat ttgtttccag attcaataaa
    16621 tctcgatata gccagggttt gcctgtacct gctcgagaaa tcataaaacc atcgcacacg
    16681 ctgatttcca gggcctttct taaactggat atattgctaa tatcaccatt tacaataact
    16741 ggtatattga cagcattctt gatatttgca atttgctgcc aattgcatgg aatatcataa
    16801 tcttctgtcc atcgcctgcc atgtactatc agggcatgag caccagcgcc ctctatttgt
    16861 ttggctaagg ttaaatcctt ttcattgttt tgaatgcgta ttttaatagt cagcggaatg
    16921 ctgatcgctg cgcgtacctt agagataatg gcaatcaatt tttgtggatc ttctaataaa
    16981 gcgctaccag cccctttttt acaaattttg cttttagggc agccgcaatt aatatcaatt
    17041 agatccgcac ccagtgattg cagtttagct gctgcctctg caaggacatg tggctcattt
    17101 ccggagattt ggtaagccag gagtttttct gtgggagctc tataaatata acgggattga
    17161 ggggagtgtt tgtgaagaat gtcgatggca gaacacattt cagaaacgca ataagcaggc
    17221 ggtgtataaa tattgaatag atctctgaaa ggggcgcaac tgtatcctgc caaaggtcct
    17281 tgaatcagtc gatggggaag ttttaaagag ccgatttgca gaggactgtt aataaaagtg
    17341 tgcatcaatg gaattaaagg atataatcga cttttatttt aacatgattc atcatgaaga
    17401 accagaaaaa agggagaggc ttccatatgg acaggaggta tttgtcacct ttggagctgc
    17461 taggcattgc tacgcaacac gcctatacag cagattatat gttgcagcaa attgccaatg
    17521 gaatgtatcg aggtggggaa accatcgcag ttttttctcc aatcacttca ctcatgtacg
    17581 ttgcttttca gctaaccttc aaagcttatt gtttgcatga tcacagacca ataaaggaat
    17641 ataaaaatct gatggaactg gttgagttga atagtcatct tggtttatcc agcaatgata
    17701 tttttctttt aaaaacttta tcaagacagc aagtgtttaa taaaggagta gattacgacc
    17761 tttgggaaaa tcagcaacaa ttgcatgttt tttgtgagga aattatttca ttgtatgaga
    17821 gagtgcagtc gatgatgcct ctggagttgc aaagtgacta tcaggagtga ccggctgaat
    17881 tagcgccctg ttaagtgtct ttgcttaagt tttcttatgt tgtgcatggt ccaggatgca
    17941 ttaatcagat atcaaaatga tttattttta tccaaaatag actcattcta cttggcatcc
    18001 caatggattc aagttaggat attatttttt taaaagggta attgtcatgg agcaactaga
    18061 cgataaagtc gcagataatg tgcaacaaaa aatactacag ttaagtcgtc atcttaattc
    18121 gcgtattttg ggtcaaaagg atttgatctc tcgtctgttg atcgctttgc ttgcagatgg
    18181 ccacttgttg gttgaaggag ctccgggttt agcaaaaacc agagccgtta aagaattgtc
    18241 agacgtagtt gaaggtgatt ttcatcggat tcaattcacc cccgatctgc tacctgggga
    18301 cttaaccggt acggacgtct accatccgca aaatggttct tttgtctttc agcccggccc
    18361 tatatttcat catcttatcc tggccgatga gatcaatcgc gcccctgcca aagtacagtc
    18421 agccctgctt gaagcgatgg cggaaaggca agtcactatt ggaggaaaaa cttatcctct
    18481 gccggaattg tttctggtca tggcaacgca aaatcctata gaacaagaag gaacttatcc
    18541 tttgccagaa gcccaattgg acagattttt aatgtatgtg aaaattagtt accctgatgc
    18601 aaaagtagaa catgacattt taactttggc acgtaaagaa gcactgggta atgcaacaga
    18661 aaatcaagtc aaagtgacga aattgtccca aaaaacttta tttgaagctc gaaaacaagt
    18721 attgaaggta cataccagtg aagcacttga aaattatctg gttcagttgg tagttgctac
    18781 ccgtaatccc ggcgtataca gtgacgaatt ggggcgctgg ttgcgttttg gagcaagccc
    18841 acgcgctact atcgcactgg accgttgttc caaggctcat gcctggcttg caggtagaga
    18901 ttatgtgact cccgatgata tacacgttat cgctcatgat gtactgcgcc acaggatttt
    18961 gttaagtttt gaagcagaag cagaaggcat tagcagcgat gactttatag attctttatt
    19021 acgtttagtc gccatccctt agaggttttt tatgactgac ggagttgtag ctgaattaag
    19081 cgaattaatc gatttaagac ggtatgttca ttccgttcat tataagccgc aaggaagagc
    19141 gttgcgttct ggaaaccata tttccaagtt tcgtggacgg ggaatggatt ttgcggaagt
    19201 aaggaattat caggctggtg atgaaattcg tcatatggaa tggcgtgtta ctgcccgtac
    19261 tggaaggcct catgtcaaaa tttatcagga ggagcgggaa aggccagtgg ttctgcttgt
    19321 agattttaac ccttccatga ttttcggtac caggatagca tttaagtcag tggtagctgc
    19381 acgattggct tctcttttag catggacagt gataaaacaa ggagatcgtg taggaggatt
    19441 ctttttttca gctacagaac acagtgagtt tattccgcga ggacgagatt cgggcgtact
    19501 ccctttattg gcttctttaa gtcgatatac cactcaaaca gaggctcagc gagaagaaaa
    19561 gccacgtatg ttaagtgatg ccttaatgag gctgcgtcgg gttattcggc ctggaagtat
    19621 actggttttg ataagcgatt tttattctat ggatgttgag tgtgagaagc atttaaatcg
    19681 tttacgaggc cataatgaca tactggccta tcatgtttgt gacagaattg aactggctcc
    19741 tccaaagccg caacaatatg ccattagcga tggtaaacag gaaatcatat tagacactgg
    19801 cctggaaact gttagtgcag cttatcagct ctattgccag cagagaattg agcagctaaa
    19861 tgatcaattt aagcggttgc aaattcagta tgtccaggtg actgctgata ccaatcttac
    19921 tcaactggtg cgcttgactt ttccccggag gtctcgtggc taaaacagaa ccattagctc
    19981 aattacgaga cattcatttg cctgagcctg ttggctggtg gcctcttgcg ccagggtggt
    20041 atggcttaat ggttcttatc gttgttttag tggcaggggt tgcctatttc atttataaaa
    20101 ggcatgttaa cgcattaccc aagaaacaag ccttaagttt attaaaaatc cacaaagaac
    20161 agtatgaaaa agataaaaac actcaattgg ccagtgccca tatttctgaa ttgctaaagc
    20221 gagttgcctt ggtttattat ccaagagcag aagttgccag tatgcatggt gaagcttggg
    20281 ttgaattttt aaatcagact ggcaagggga ttgattttac cccggttaaa tcgatgttat
    20341 tggattcacc ctataaaaca tcagatgcac tgaatttaaa tcctttattc actcgtgcag
    20401 aaaaatggat caagcaaagg ggggcgccat gttcgaatta gctaccccat gggctttgtc
    20461 actcattcct gtccctttgt ttctctggta tttgttaccg cgggcaaaaa taaaattacc
    20521 tgctgcattg aaggttcctt tttttgaggc aatggcaggt attgttgaac aagagaaccg
    20581 gttaatctcg gctaaaacgc tgctattaat tcctgtcttg gtttgggtat tgctggttat
    20641 tgctctttct ggtccgcgat gggttggtga gccaaagcct gtagcaaggg aaggatataa
    20701 tatcatgatg gtattggatc tttcaggcag catggaaatt acggatatgc tgctgcatgg
    20761 ccgaccagtc agtcgtttgc ttgtcgtgaa aagagccgca gagcaatttg ttgaggacag
    20821 agtcggagat agaattggtt tgattctgtt tggtactcga gcttatttgc aaacaccatt
    20881 aacctatgac agacatagcg tactaatgag gatagacgac gcaacagctg gtcttgcagg
    20941 taaaactact tcgattggtg atgcagtagg tttagccgtc aaaaggttac aagatgttcc
    21001 ttccaaagga agagttatta ttttgctaac ggacggagca aacaactcag gtgtattagc
    21061 ccccttaaaa gcagcagagt tagccaaaca agatggaatc aaaatctata ctataggctt
    21121 aggttcggag gccgatccaa gagcgttaac tggtgatttt tttgccccca ccctttccgc
    21181 cgaactggat gaaaagacat tggaaaaaat ggcaaaaatg acaggtggtc gttatttcag
    21241 ggcgacagat cctgagtctt tacaatccat ataccaaacc atcaatcaac tggaaaccgt
    21301 gaagcaagaa caggcaactg ttcggcctca aaaggaatat tatccctggt tcgttgcctt
    21361 tgctttgtta ttatgctttt attggttgat tagcaaggcg gatttatttg tgtacttaag
    21421 gtctttttta aaagtagagc ggagcttaag tcatgatagc tgagtttcat tttttaaggc
    21481 catggtgttt gttgatgata gtgcctttgc ttgctttggt ggtaatttat tggcggcaaa
    21541 aaccaaaatt acatgcctgg gaggcggtat gtgatcccca tcttctggaa catttattac
    21601 acagtaaagg tcagagtaaa cgtgccttat ctttatcatt cctgtttttt agtattttat
    21661 ttatgatttt gagtgtgtct ggtccaactt ggtacaaatt gcctcaatct acttataagc
    21721 caattcaacc aagggttttg ttattggata tgtcagataa aatgatgctt ggggatttat
    21781 cgccaaatcg tcttagccgc gccaaattca aattgcatga tttatttacc cgcaaggata
    21841 tagggcagtt tggtttgatt gttttcacag gcgaaccttt tgttgtatct cctttaacag
    21901 acgatggtca aactatctct tccttattgt caaccttgac tcctgatatc atgcccgttg
    21961 caggatataa tttagacagt gccttggatg aagcaagcca attaattaag caagcgggtt
    22021 atgaccatgg cgaaatttta gttttaaccg ctgaaacacc atcctctagc gctattgaaa
    22081 aagctaagaa gctggcagaa tcaggtattt tttcttcaat tatgccaatc agagctgatg
    22141 aaaatttgaa ccccttgttt cagcgcttcg ccaatgcggg agatggatac ttgttggtat
    22201 actcaccaga ttcaagtgat ttggaaaagt ggttgaacgc ctctcaaggc aaagaaaaat
    22261 ttgctcttga aaaaaatgaa gatatcccat tatggcgtga tgaaggccgc tggttcttga
    22321 ttcccgcatt gcttttatta ttacccgtat ttcagcgagg ttggatgcaa agggtgacgg
    22381 tatgatgtgg cgggttttaa ctattgtggc agctgcacta atatccaatc aacttaaggc
    22441 atttagttgg tctgatttat ggtttactcc caatcaacaa gctcaacatt tgatggaaaa
    22501 gggacaattt gcaaaagcta aagatctttt tgagcgcaat gactgggctg caagtgcagc
    22561 atacagagca ggagattatg aacaggcagc caaattattt aatgagttaa aaaccgaaca
    22621 aggctactat aatcagggaa atgctttagc gcacttaggg aaatatgagg aagcaatacg
    22681 agcatatgac aaagccttgg catttaatcc aaataatcag gatgctttat ataatcgaga
    22741 attaatcagt gaattgttga agaaagataa agaaaagaaa aaaagccagg atcagcaaaa
    22801 tcaggacaag caaaacaaag atcaacagaa tcaggatcag aaaaatcagg acaagcaaaa
    22861 caaagatcaa cagaatcagg atcagaaaaa tcaggacaag cagaacaaaa atcagcagaa
    22921 ccaggatcag caaaatcagg acaagcagaa caaaaatcag cagaaccagg gtcagcaaaa
    22981 tcaggacaaa caggacaaaa atcagcagaa ccaggaacag caaaatcagg acaagcaaaa
    23041 caaagatcag cagaaccggg atcagcaaaa taaggcacaa cagaataaaa atcaaaataa
    23101 aaaaaatgac aagcaagagg gagaaattca gtccgaatcg aatcaggaga agcaacttgc
    23161 taaagaacaa tggttgcgat taatacctga tgatccgggt ggattaatga gagaaaaatt
    23221 tttacgtgat catttacgta gagagcgtgg gtggtatcaa tgaagaaatt aacgttatgg
    23281 gttttattga gtttattttc atcattatct tttgcagaaa tcaaagtcga acttgaacct
    23341 tcgcaagtca caatgggaga aacctttaaa ttatccatta ctcaagaaga tcaacagggg
    23401 ggcggagttc ccgatctgac tgaattgcaa aaggattttg tgattttggg tacagaaagg
    23461 catgtaaact attccattat taatgggcag gcgcaatcac aaagtcaatg gattgtactc
    23521 ttgagagcgc taaagccagg tatttttact attcctccaa taaaagtagg tcttgaacaa
    23581 agtctgccaa tcactatcaa tgtccagggc agtgccaata cacaagcttt gccagatgag
    23641 gctatacaaa agcaggacgt tttcctgaca gctgaagtga atgagaaaaa cccctatgtt
    23701 aaccaacaaa ttatttatac ggtcaaatta tacaatagca aacgattgtt agatgctgat
    23761 tatcaaggtc caaaagttga tgatgccttg ctgattccgt taggagatgc gaaacgttac
    23821 catgctgttc ataacaacat taattatgtt gttgaagagc aaacttatgc gatatatccg
    23881 caaaaaagcg gcactttgaa aatcatatca ccgattttta atgctcttgt atatgactat
    23941 aacccccaac gcaccaaagc gacggataaa gcgattaact taactatcaa gccaatccct
    24001 aaaaagtatc aaggcaaatt atggttgcca gcaaaacagg ttaagttatc cgagcaatat
    24061 gaaaacagta atcagactct cagtcaggga agtacgttaa cacgtacagt gacattggaa
    24121 ggaataggca ttccagctca gttattgcca acacttacct ttcaagaaac agatttcttt
    24181 agcgtttatc cagagaaagg ggcggaacgc aatcaggtta agcaaggtga gctaattggc
    24241 agtgtcgaaa tcaaggtcac ttatttgttc aacaaagcag gcaaaacgac aatacccgaa
    24301 ttgcgtcttc cctggtttaa tactgaaaca ggtaaagaag aaatcgctgt tttacctcca
    24361 aggagcatag atatcatccc ttctactgcg gtcaaggcaa atactgcaaa cacccaagtc
    24421 acaggtaaag aaatgtctgc cagcagtcaa tcagttcctg attctgcatc agtttccaat
    24481 gaatcaggag gttgggctgt gaagttggca atattttttg gtttggcctg gttaatcact
    24541 ttaagcttat ggatatggca aaagcgaccg agagagtcat ccagtaaaaa ggagtataaa
    24601 aaagctttgg atgctttaaa taaagcttgt agtcaatgta atcccaaaaa agctcgtgat
    24661 gctgttttaa aatgggcctc tttgcaatgg cctgatgctc caatgcttaa tttaactgac
    24721 ttggcgcaat tggtaaggga cgcgcattta aggaaacaat tgcatctgct ttcgcaagtc
    24781 ttgtaccgaa gtcaggaaaa aacattatgg cgcggggatg aattattacg ggctgtgcaa
    24841 gcggtaaaac gtaacgaatc tgtgaaaaag cgaaaaaaca gtaagttacc tcctattaat
    24901 ccattgtaga aattaaatct gtttaagcgg tggtttttgc cagttattcc tttactgtca
    24961 atacggacaa cttatttatc cccttttttc tgtaatcacg ggactactgc cagattgtat
    25021 gccgtcataa tttggttaag ctggctcagg cgaccatgat aacaacagct ataatgttat
    25081 agatatcctt gtaaagagta atgttctctc tttataaagc tctctggcta ttgtaggaat
    25141 tgagtatgca tgttaatgag tatatccact atgatgtgca ggagctcgct gaactgatca
    25201 aaaaccatca ggttggtgct gaggaagttt tgcggtgtgc gcaagaacgc ctgtatcaag
    25261 tgaatccttc attaaatgcc gtagttacag attgttttga atatgcgcat caatgccttg
    25321 caaaaatgaa aggtaacgag ccctattatg gtgttccgtt attagtcaag gatctgggcc
    25381 atgccataga gggcattcga ctaacggaag ggtcaaggtt tttttcaaaa aatcttgctg
    25441 aaacaaacag tgatttgatt aataaattga ttgctcttgg ttttgtacca tttgcaaaaa
    25501 caaatacttc agaattaggc ctttcctgcg ttactgactc taatttattt gggccttgca
    25561 gaaatccttt tgatactatg agaacggcag gcggatcttc tggtggttct gctgctgcca
    25621 taatggcagg gatagcgcct gtggctacgg caagcgatgg gggcgggtcg attcgtattc
    25681 cagcggcttg ttgtggtttg tttggattca aaccgacacc aggtctggtt ccctcagggc
    25741 catgggttga tgaagcatgg tccgggatgg ctaccagttt tatactcact cgttccatga
    25801 gtgatgctat tgcattgttt aaaaatgtaa taaaccccat gcgacttaac agactatcga
    25861 gaaagaaaaa aaaattgctt attaccgagt tgacaggcgc ttttgccgag gtaccaattg
    25921 caagggaatg ccaggaggcg gtagagaaaa taaaagaaat cctgaaaaat tcaggccaca
    25981 gattaaatga agtgcacttg ccattggatc tgcaagttat cagttcatgc gcccatactt
    26041 taattgcagc gagcacgtat gcgaaagtaa aaactcaaga acaaatcagt ggcagaaaac
    26101 caatgattca tgaattggaa cccataacat gggaatttta ttacagagga aaaatggtct
    26161 ccgcttatga attcattatt gccaaaaaca aactatacca atcactaaga cctctgcatc
    26221 aattattgga aaaaacagac attgtattaa cccctgcatt ggctcaatta cccctgttaa
    26281 tcggtcattt gcgaattgat gaggaattca ataagtatct acaaaaaaag aaagagtttt
    26341 caccttttac ctccctgttt aatcaaggtg gattacccgc tatgactgta ccagttatgt
    26401 tccaccatca tttgcctgtc tctgtgcaaa tgggttcagc aaaaggcaat gatttgctct
    26461 tattggagtt ggctcatgaa ttacagtctg tgctgccaaa aaatatcacg cctgcatcaa
    26521 ttccttgatg ctgatttttt gtaagttttc atttatcgaa ccttgtgtta agttaactgt
    26581 agcttttcat aaaattaaga ataagtgatt gttttttata gctatagtca cttgaatttt
    26641 aaataatcga ttctctacgt actgctgctt aatgcggttt ttaaaaatca agtcactata
    26701 tattttttca acttcagggc attttgcgta ggcagtaaat agttccagtt tgagtagttg
    26761 tatttaataa aggtaagttg taaatggatt tcatcaaaga ggatttcaat gaagacgtta
    26821 ccatccaaag tcatttttat aggggcaggc ccgagcggct tattcgctgc acgccaatta
    26881 aaggaaatag caaaattaca aaataagaca gtagaatgta tattccttga acgtgaacct
    26941 gtagtgggcg gcaaatgcca cacctactcg gatgcagctc atcctgagtt aaaaacggaa
    27001 tggggagccg cattggttgc tcccaactat ggtgttgttc ttgatgcatt agctgagcac
    27061 ggcattgaat ttgaaaaggt aatgccaaca gcttctgaaa cgatagaaat caagcaatta
    27121 tttgaaaaag ccagttcgtt agaaaaaata agcatggaaa tgaagttgct tggagaatta
    27181 tgggatttta atagtgatta tgatgtttat aaaaaggcaa aacaaaataa aaaagcactt
    27241 ccagataaat tgttaaaccc cttttctgaa tattgtaagc tgaacaacat gtcttattta
    27301 cctgtccttt taaagccatt tgtgcctgga tttggttacg gggatttaaa cttttgtccg
    27361 gcttattgtg ttcttgagta tatgggcaag gtcaccatgc cggacatcct cttagccgat
    27421 caattaatcg gcaggccctc actattagca atacatggcg gttttcagct tttaatggag
    27481 agaatagcgg aacaatttga tgtgcgtacc agtgcttctg tttccaggat taagagagaa
    27541 aaaaataagg tgactgtgac ttatgtgcat aatggtatcg agcaaacaga aacagccgat
    27601 actttggttt tggcgacttc accaaaaaat tggaaatctc tgggtttaga tttaactgaa
    27661 gttgaaaaca attgtataga gagtctggat tattatcgct atcctgttgc tgtttataaa
    27721 atcaaaggat tgccgccgca acagtatttt ttcacaaaag cacttgagga aaggggcttt
    27781 gggcatttgg cgctaattac aaccagggat aacaggagta atccggaaga tggacggtta
    27841 tgcacggttt atgtgaattt atctcccaat aataatgaat ttaaatttga tcatgaagcg
    27901 ctaagaaaag aattagagtc tatcccggat gtaacggaag ttacggttgt aaaggataaa
    27961 atctgggaag attatatgtc gactcttccc tgggacttgc gattgcaatt ggataaagag
    28021 caacagcgca cgaatacaat gtatttgggc tcttatgctc tgggaagttt tgaagacgta
    28081 gcctgtgtgg caaataaggc aaccgatgcc atgacggaat ggtatgcccc cactatgagg
    28141 tatgaagagg atttcagctg gaaaaatatg aatagggcct ggcagttttt tacatcgcca
    28201 acgaaatcgc cacttgcttc gttgggtgag cccaggatgt cggatgaaca cagtaacagg
    28261 ccatagacgg taagataaaa gtggtacatt tatcgaagat attgtacata atgctcgcta
    28321 tttgtagaat agtgtattat aatataatta cttgttctac gagcattatt ggttaaaatc
    28381 ttgtttttcg acaaactcct tccttatatt tcccttggtc gtgaatttct ctcgatgatt
    28441 acattgtatt ttttctctta tcgctcatta caagtgtttg tttaatttta tttggaactt
    28501 tgaaatgtct gtgaaagaaa ctggccacgt taagtggttt aatgatgata aaggttatgg
    28561 cttcattagt cgtgataatg gtcaagacga tgtatttgta cacttccgct ctatcgttag
    28621 cagcgctggg cgcaaaactt tgctagaggg acaacgagtg gaatttcttg ttactaaggg
    28681 cgcgaaaggt ttgcaagcag aagaagtaac tgctatataa tcccgtattc actgaattac
    28741 cttcctcttt gtctaaaaaa acgaagagga aggtggtttt ttaatgccaa taaattgcat
    28801 taattttttc aattattttt cactttctct taagaatttg taccgagacc tgtttggtat
    28861 aaaagctggc agtaagataa cgatattcca ctttattttc ttttacccat tcttggaaag
    28921 ctttaaattc atcctggcgc caattgatat taccaatgta ctcatcgaat actatgatag
    28981 tacctggtgc aatctggggg tttaattcat tgaacaccaa ttttgtggaa gaatagagat
    29041 cacaatcgat attcataaaa gctatgggtt ccctgttatt ttttttaaaa tctggcaaag
    29101 tttcattaaa ccatccagcg attaaagtca cattacttgc tacggaggga attttaccgt
    29161 aggtggaata ggagccagcc ttttcgtggt gccaatcttc aggtagccct tgaaaactgt
    29221 caaaaccgaa aattttgcgt ttgggaaaga gtttggcaat atgggaaatg gaatagccaa
    29281 atcgcactcc aaattccagt attaagcctt cagaaagaac ctgtggtgct aataaatcaa
    29341 atgtctgata agttgttcca ctatgtcgca aatgtttgtc agcatgttta tgaagataat
    29401 cagctgcatc aatgagatca aagcgtttga atgcgcgata cgtttctatc ggtgtcttgt
    29461 ttaaaccatt gagtatgtct attgcgattc gctggcagca aaaataatca ttattgggta
    29521 tgcgttgaat tgctctgtct aaaagagatt ttactgcttt cttatcattt aaagccagat
    29581 ataccgcatg cagtaaaatg gtgatgtgtc catccttatc cgttttttca agtggttcaa
    29641 gcaagaagcg cgcttcctct actttattaa ggtcaagcaa aataatggct cttgcccaac
    29701 ttgttttggg gatattgaat tggtttacat aagggaacaa ggaatacgct tcttgtaccc
    29761 ttccttgttg ccttagagca tagaggtaat atgcgaggct ttcaccgtcc atttcttcaa
    29821 ttttaaatga ttcaaaaagc ttgcaggctg acaaagaata attagcatgc gccagagttt
    29881 ttgctgtcat taatcctatg tcagttttga attcgctgaa taaataagct tgccagtaca
    29941 tggctgctgc ttgtattgtt ttacccatgc gaagtaaaac ggttcctttt tgataatagg
    30001 ccccggaaaa ttgtgggttt atcgctaatg cttcatcata cagtgttaag gcaagaggca
    30061 aatccccctt ctctaaagcc tgatctcctt tggatttaat cactacagga tcttgttggg
    30121 tcatttattt tctccagtac ctacctctat atcatagtat tctttatatt aggaatgtag
    30181 ttgtattaaa aaaatcctta ttaatcaatt tcatgaaaaa ataattgctg gcattcatat
    30241 ccgactaggg tataatgggc ttaccaaaga caagccagga tgtcgtaatg ataaaaccaa
    30301 tcaccctgct tttgcttttt tgttctttca ctgttaatgc ctcaattctc gtcaatggta
    30361 ctttcgaagc aacacaatca tgccctgctt acctttccaa gaatcgtaaa tcaaatcctg
    30421 ataatttaat ggttgagccc aaccagaaat atcaattaaa agaaattaat aaaaccaaac
    30481 cggattggtt gcgtatacaa ttttcagacc gtcaacctaa tctgcgctgg gtgagtgtgg
    30541 attgcggggt cattgaatac actgagcgtc ccaaagcgag ctgtgatctt tctcccggga
    30601 tggctgattc ttatgtgttg gctttaagtt cacagccagg tttttgtgaa acttatggat
    30661 atgaaatagg caaaccagaa tgtttgaaat taacaaagac agcttatcag gccaatcatc
    30721 tgatcctgca tggattatgg cctaatcagg aagcatgcgg ccataattat ggctattgtg
    30781 gaataagccc tcgaacaaac cattgtgatt attctccatt ggatttatct tctgatgttg
    30841 ctcaaaattt aaagaaactt atgccgagtt ataattatgg cagttgtctg gagcgccatg
    30901 aatggaacaa acatggaagc tgtcaggatt taagcacaga tggttatttt agtttggcca
    30961 tgcgcttgac tacagaagtt aataatacag agttcggtca atatcttacc tctcatcgtg
    31021 gtgatactgt aaaattggct actttgcgtg aattgattga aagatctttc ggggaaaaca
    31081 atacaggtaa agtgacttta agttgcaaga atgggatctt ggtggatatt tatgtccaat
    31141 tgcctgctct catacctttt aaagagtctc tagagtcttt ggttgataaa gccagccact
    31201 cacaacacca tgattattgt aatagcaatg tcaccatttc tgattttagt aatgaaaaat
    31261 ggttataggt attcaagtgg aggagatggc tcgttcacaa aacttttttt aaaataatat
    31321 ggcattgctt ccttatagga gactgccttg cacattgtca aaacttcctc taatgtagag
    31381 agattattga tataggaata cagattatgc taaagcggtg ctacttattg attttactta
    31441 tgtttgtatt ggcaagctgc gcccaccata aaacgcaaac acctccagcg gaggttaaaa
    31501 aacaaagtac cagcagtaca cggcagtttc gccaagtgtc atcgttcaat caaatcgtag
    31561 ttcagggacg actgaatgtt aatttgcata ccgggtataa taagccagag gtcatgttac
    31621 gcggtgatcc cagagatttg gttcaagtaa gaacagtagt aaaacaaaat acactctacg
    31681 tgtctttggg ccagggatac ccggattatg gtactgtgac tgtggatatt aaaaccaaat
    31741 tcctcaatcg gtttcgctat gagggtgctg gggttgtgac agggaataat ttacggacca
    31801 gctatttgga tttatatttg gcgaacgaag gcaccaccag actggcagga aacattgggt
    31861 tgcaaaaact cgaggcagtc ggtaatggtg tgacacaaat caatggtgta tccagcagga
    31921 atttacaaat cgtattgaaa ggagatccca aagtactgat atctggtttt gtcaatctaa
    31981 gacagcttga tatgtatgga aaaggcacgt taagtttgta ttggataaaa agcgatactt
    32041 taaccattag agcaaaaaag gcggccaaaa ttcaattggc tggaattgtg aatcgactgg
    32101 atgttgaatt gtgggatttt gctcaattta aggggaaata tttgcgagct caacgtagtt
    32161 ttgtgaaaac ccatgataaa tccgtagcag aaatttcagc tgtgaatcat caaagcagtc
    32221 ttgctactga tgccagtgat atttactatt ataacttatc aaaaaccagg gctgatttca
    32281 tggcatttaa tggatccgtc ttggatatgc gcgaatggag ccaatctgat ctaaaagatt
    32341 ttgatcgcta taataagcag tttccctagc atttattcag gataatagag ctaaattcat
    32401 tagggtaagc ccttggtgaa tttagctttg tttgattaaa gtcggaatgg gagcccaata
    32461 ttgacttcaa atatttcctc atcagacgtg aatggatcat tagagggatc gttcataagc
    32521 tgacttagaa aagagggaga gacatcctcg taggactctt catcactata atttccaatg
    32581 agtaataatg aggctaatcc tgtaacgaga atagcaattt ttgatgcatt taattcatca
    32641 ttttttaatg aggcattata aaaaaacatg tttaatttcc aaagaacaaa atgagtcgtt
    32701 aaaatacaat gatttttttt cttattcaat atctaaatgg ttagaacagg gaaagtccaa
    32761 atgccatgtt ttatgattga ttacaagtca attat
//
LOCUS       DBNCWK010000022        30563 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.022, whole genome shotgun sequence.
ACCESSION   DBNCWK010000022 DBNCWK010000000
VERSION     DBNCWK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 30563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30563
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.022"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..1087)
                     /gene="lepA"
                     /locus_tag="AC06IO_000925"
     CDS             complement(<1..1087)
                     /gene="lepA"
                     /locus_tag="AC06IO_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LepA"
                     /protein_id="HIO9874135.1"
                     /translation="MNEIILHLMNEISTLTPPINPLNLKQIFSSNLLPEGKAPVEVES
                     QLNLLLLIKKYVDEYSSLAEEQEEYKRKKHKLDEVMKKGEELQRKISDLAKKIEHAKN
                     ELNPLYKEIEPKANEVLSKQNPLAPPYKFPEYGEVQKSIVVGHYSYLFKTSKEIETTR
                     AKLKLVLLKNKWNALMADLSQFGIQPPVEEDAVDSLQKFIAAVELEIKKLTRACEKWD
                     ELQKRFAKQLSATDINKKENELEKQIELLLKDLGRIETVVTNHSLAPDLKAELTRKFQ
                     ESEDTQGLIQEYQNQIDGLLSNFNPSSWLSWYSDSNYSDNQQKLKNSVSFLQLLAQQR
                     ELKTKHHELVKQQELLIKAIPETPSNEE"
     gene            1331..2080
                     /gene="tpiA"
                     /locus_tag="AC06IO_000926"
     CDS             1331..2080
                     /gene="tpiA"
                     /locus_tag="AC06IO_000926"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIO9874136.1"
                     /translation="MRQKIVAGNWKMNGQIQQVTELVSQIEELIGFDCAAQVAVMPPS
                     IYIPKVRDCLRTGKIVVGAQNVYPKDYGAYTGELSAPMLKDFDCRYVLVGHSERRQFF
                     HEDENFVAQKFHHVKDHGMIPVLCVGETLSERENGKTEQVIAQQVLAVSAKGKDCFRD
                     CVVAYEPVWAIGTGKTATPEQAQKIHQFIRDLVGEINDSDAKHLTLIYGGSVNENNAK
                     ALFSMPDIDGGLVGGASLNAKQFVEIVKCIN"
     gene            2068..2391
                     /gene="secG"
                     /locus_tag="AC06IO_000927"
     CDS             2068..2391
                     /gene="secG"
                     /locus_tag="AC06IO_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIO9874137.1"
                     /translation="MYQLILMIHVLIAVILIGLVLIQHGKGADIGAAFGSGASNTLFG
                     SQGTGGFLFKLTGGLAMTFFITSLMLSYMVSTQNQKAGQLAIPQQTGAPVSSVPVPVE
                     KNNKK"
     gene            2462..2546
                     /locus_tag="AC06IO_000928"
     tRNA            2462..2546
                     /locus_tag="AC06IO_000928"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2496..2498,aa:Leu,seq:gag)
     gene            2571..2927
                     /locus_tag="AC06IO_000929"
     CDS             2571..2927
                     /locus_tag="AC06IO_000929"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIO9874138.1"
                     /translation="MLSQYLPVLVFIIVALILGAVMIGAGSLLSKHSPDSAKNSPYEC
                     GFGAFESSHIPFDVRFYLVAILFIIFDLETAFFFPWALALRKIGWFGFYAMMLFLGLL
                     VIGFIYEWKRGALEWE"
     gene            3175..3651
                     /locus_tag="AC06IO_000930"
     CDS             3175..3651
                     /locus_tag="AC06IO_000930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HIO9874139.1"
                     /translation="MAVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMH
                     VGAARYDLDRFGIIFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA"
     gene            3674..4357
                     /locus_tag="AC06IO_000931"
     CDS             3674..4357
                     /locus_tag="AC06IO_000931"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIO9874140.1"
                     /translation="MTKNEYLIEKLQVDLANHITELTSAYGEVTIECEVQNLLPVMIE
                     LRDREEFSFDELIDLCGVDYLHYGDYDWETESATEHGFSRGVERQEAKAYAVNKPRFA
                     VVYHLLSTKKNHRLRVKLFVEESHLIVPSVHHLWKSANWFEREAYDLYGILFDGHPDL
                     RRLLTDYGFIGHPFRKDFPLSGEVEMRYDAKLQKVIYAPVDIVPRIVVPKVIRNDNRY
                     IGNEGSKND"
     gene            4350..5603
                     /locus_tag="AC06IO_000932"
     CDS             4350..5603
                     /locus_tag="AC06IO_000932"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043880841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIO9874141.1"
                     /translation="MIELKNYTLNFGPQHPAAHGVLRLVLELEGETIVRADPHIGLLH
                     RATEKLAETKPYIQSIGYMDRLDYVSMMCNEHAYVMAIEKLLGIEPPLRAKYIRTMFD
                     EVTRILNHLLWLGATALDIGAMTVFLYCFREREDLFDCYEAVSGARMHATYYRPGGVA
                     RDLPDTMPQYKPSRWHSEREIEKKNHNRQGSLLDFLWDFTERFPHCVDEYETLLTDNR
                     IWKQRTVDIGVVSPENALQWGFTGPMLRGSGIAWDLRKKQSYAAYDRVEFDIPVGKTG
                     DCYDRYLVRVEELRQSNRIIRQCIEWLRKHPGPVKVDDYKVSPPRRVVMKHDMEALIH
                     HFKLFTEGFCLPRGEVYSSVEAPKGEFGIYMVSDGANKPYRLKIRAPGFAHLSSFDDM
                     VRGHMLADGVAILASQDIVFGEIDR"
     gene            5664..6167
                     /gene="nuoE"
                     /locus_tag="AC06IO_000933"
     CDS             5664..6167
                     /gene="nuoE"
                     /locus_tag="AC06IO_000933"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIO9874142.1"
                     /translation="MSANSAKILNQLMPANRMSDIDHWISKYPKDQKQSAVMSALRIV
                     QEEHGHLTTELMNAVAEYLDMPPIAVYEVASFYTMYEHKPVGRHLINVCTNISCMLRD
                     SAGVVEHLEKKLGVNLGGTTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESID
                     KVLEQYQ"
     gene            6186..7463
                     /gene="nuoF"
                     /locus_tag="AC06IO_000934"
     CDS             6186..7463
                     /gene="nuoF"
                     /locus_tag="AC06IO_000934"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIO9874143.1"
                     /translation="MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQL
                     IIEELKTSALRGRGGAGFPTGLKWSFMNRNSPVQKYVVCNSDEGEPGTCKDREILTLN
                     PHQLIEGMAIAGYVMGATVGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVD
                     FDLYNHLGAGAYICGEETALLNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFAS
                     VPVIMEKGGEWFLKLGKPNNGGAKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIK
                     GRKIKAVIPGGSSMPVLPGDVMLGLDMDYDSIQKAGSGLGSGAVIIMDETTCMVDALY
                     RISEFYMDESCGQCTPCREGTGWLVRLLHRIINGHGEPGDVEKLVNVANNIEGRTICA
                     LGEAAAWPVQSFVKHFYDEFEYFIKHKRSIVAA"
     gene            7480..9831
                     /gene="nuoG"
                     /locus_tag="AC06IO_000935"
     CDS             7480..9831
                     /gene="nuoG"
                     /locus_tag="AC06IO_000935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIO9874144.1"
                     /translation="MATIEIDGKTFEAENGKMIIEVADEAGIYIPRFCYHKKLSVAAN
                     CRMCLVEVENGRKPVPACATPITNGMKVFTKSEQAIHSQKVVMEFLLINHPLDCPICD
                     QGGECELQDISMGFGADKSEYTESKRAVDDENLGTLISTEMTRCIHCTRCVRFGEEIA
                     GVRELGATGRGEKTQIGTYVEHSMTSEVSGNIIDLCPVGALTSKPYRFKARAWELTQN
                     DSVAPHDCLGSNVHIHTRNNKLMRVVPRENESINETWLSDRDRFSYLGLNAASRASKP
                     QIKKNGQWEPVDWETALKFAAEGISRVIKQHGPEQMAAFASSSSTLEEMYLLQKLMRE
                     LGVQNLDHRLQQIDFRDQAFLPTTPVSTLPYAEIDHQNSILLIGCNIHREVPLAGTRV
                     RKAFRNGAKIYALNPVDFDYHFDLSGRVIISPLEMPMQLAKLALALTTELTSLPEEVQ
                     KLLIGLEVDKQTKQIAQSLKEEKACLITGAIVENHPEASLLRTLVAIVQKLSGAKLVR
                     LTTGANSAGACIAGMLPHRTVAGKSIAEPGFNVQEALNSKLKGYLLMGVEPGYDFANP
                     AGARQSMLAAEFVVLLSAYEHESMHDYADVILPIAPYAETSGTYINIDNTWQTVKGAM
                     LPFGESRPAWKVLRVLGNLLHCKKFDYTSTEDILEEVKEAVSMTMEHEYEPYYPESLP
                     VINQSLVRVGEWPLYRIDAITRNAKELQLCAASESACIRIHPSTADRLKLEEIATVSQ
                     GDIEITLPLKRDERIAVDVVWVANAMPETVDLGHSFAAITIKR"
     gene            9844..10866
                     /gene="nuoH"
                     /locus_tag="AC06IO_000936"
     CDS             9844..10866
                     /gene="nuoH"
                     /locus_tag="AC06IO_000936"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIO9874145.1"
                     /translation="MLHTIGILIWIIIKILVIVVPLLISVAYLTYAERKVIGYIQVRI
                     GPNRVGLKGLLQPFADLLKLITKEIIVPTRSNKYLFVIAPLFALVPSLVGWAVIPFQE
                     GIVLANINAGVLYLFAMSSLGVYGVLIAGWASNSKYAMFGALRSTAQTVSYEIAMGFA
                     LVGVLLAAGSMNLTDIVNSQKGGMLHWWFIPLLPLFLVFWISGIAETNRAPFDLAEGE
                     SEIVAGFHVEYSGIGFALFFLSEYASMILISTVLAILFMGGWLSPFEGITFLDQIFFI
                     VPGFVWLLLKISFFLFVYLWVRATFPRYRYDQLMRLGWKVLIPVTIVWLIVTAVMVVA
                     HVKPWF"
     gene            10885..11385
                     /gene="nuoI"
                     /locus_tag="AC06IO_000937"
     CDS             10885..11385
                     /gene="nuoI"
                     /locus_tag="AC06IO_000937"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIO9874146.1"
                     /translation="MKKVYHYIIHYVRTYLLLELLAGLWLTVKYFFRKKITVQFPEEQ
                     TPLSPRFRGLLALRRYPNGEERCIACKLCEAVCPALAITIESEQRDDGSRRTTRYDID
                     MFKCINCGLCEESCPVDSIVVTPIHHYHISERGQNIMTKEKLLAVGDLMESQLAADRA
                     ADEKYR"
     gene            11397..12056
                     /locus_tag="AC06IO_000938"
     CDS             11397..12056
                     /locus_tag="AC06IO_000938"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIO9874147.1"
                     /translation="MHEWVIQAIFYVFALLTVLSAVMVISQNNPVRCVLFLVLTFFTS
                     AVLWILAEAEFLALILILVYVGAVMTLFLFVVMMLNIDIESMKSHLIKYLPFGLIIVA
                     LLTGLLMVAIPKDLFKNSVQTQEKPLSVNSQLMDLSTEDGLVTPKNATSNTEKLGMVL
                     YTDYILAFEIAAVILLVAIVSAITLVHRGAIRSKRQDITQQIMTRRAERVKLISMKSE
                     K"
     gene            12074..12379
                     /gene="nuoK"
                     /locus_tag="AC06IO_000939"
     CDS             12074..12379
                     /gene="nuoK"
                     /locus_tag="AC06IO_000939"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIO9874148.1"
                     /translation="MIPVYDYLVLGVILFGLSLVGIMLNRKNIILLLVCVELMLLAVN
                     TNFIAFSHYYNEVGGQVFVFFILTVAAAEAAIGLAIVMLLYRNRGNIDVDKMNHLKG"
     gene            12384..14357
                     /gene="nuoL"
                     /locus_tag="AC06IO_000940"
     CDS             12384..14357
                     /gene="nuoL"
                     /locus_tag="AC06IO_000940"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIO9874149.1"
                     /translation="MSIQQVCLVIVLTPLVGSAIAGFFRNQIGRVGAHTVTILGVAIS
                     LACSIFLAWGLFSGSIPNTNSLVYHWASGGALIPYEFNIGFLIDPLSVVMLVIVNFVS
                     LLVHIYSIGYMADDDGYQRFFSYISLFTFMMLMLVSANNFLQLFFGWEGVGLVSYLLI
                     GFWYQKESAIEGSLKAFLVNRVGDFGFVLGIGLIFAYAGSLDYEKVFTSANYLASQNI
                     ELFSGYSWSLITVICLLLFVGAMGKSAQVPLHVWLPESMEGPTPISALIHAATMVTAG
                     VFMIARISPLIELSTAALSTVLVIGATGALFTGILALVMNDIKRVVAYSTLSQLGYMM
                     VAMGASAYSAGMFHLLTHACFKALLFLGAGSVIIGMHHEQDMRKMGGLWNKMPITYVT
                     YVIGSLALCAFPPFAGFYSKDTIIEAAQLSQIPGSSYAYFCVTAGAMVTALYTFRSLF
                     MTFHGKPRMDEHTLSHLHESPWVVWLPLVLLAIPSIILGYVLYMPILFDTPSLLGSSI
                     FVLPEHNVLAELAHEVSSPFASAVHAIYSLPFWITVLGAVIAWICYIAVPSIPGYLAR
                     YFSIVYSILLNKYGFDRFNELFFVKGARGLGIAFYKIGDQKLIDGAVVNGSGRLVRWF
                     SGKGRKIQSGYIYHYATVMVFGLLAFLCWLILD"
     gene            14373..15878
                     /locus_tag="AC06IO_000941"
     CDS             14373..15878
                     /locus_tag="AC06IO_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIO9874150.1"
                     /translation="MHYLLNLLIWLPILGGVFVLLTGDDKNPNVSRYLTLFTILLCLV
                     LCIPLVKGFDLQSFGMQFIEEIPWMPALGINYTLGIDGLSLLLIVLSIFTNLIVILAT
                     WDSISKKVSQYLSAFLIMQGLLVGIFSAMDAIVFYIFWEATLIPMYLIIGIWGSDNRV
                     YAAIKFFLYTFLGSVLMLASFLYMGYIAGSFKIETFYAIKLGMTAQTLIFIAFFLGFA
                     IKIPMFPVHTWLPDAHTEAPAGGSVVLAAILLKLGAYGFIRFSLPIVPDACSKYAPLM
                     VALSLIAVVYIGLIAIIQKDMKKLIAYSSISHMGFVTLGCFAIFAIARHGGLSNAGLI
                     LEGAIVVMISHAFVSSAMFAGVGFIYDRMHTRQLADFGGIVNTMPIFASFFMLFAMAN
                     AGLPGTSGFVGEFMVILGSIKAGFWVAFWAATTLIIGAAYTLWMYKRVIFGPVANKKV
                     AALKDISGFELSAYVLLAIMVIALGVYPKPMLEFVHQTVSHTLALADKSKL"
     gene            15932..17374
                     /gene="nuoN"
                     /locus_tag="AC06IO_000942"
     CDS             15932..17374
                     /gene="nuoN"
                     /locus_tag="AC06IO_000942"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HIO9874151.1"
                     /translation="MMTLLENIHVAIPEMIILITACLALLSDLFFRHKLKSIAFYISC
                     VGIIVSAAVSFVFIGSYKLLIFNKLFISDDISHLMKLFIDITVLLSFVYSQNYLDERQ
                     MPTGDYYVLGLFSTLGMMILVSAHSLLSLYLGLELMSLPLYAMTAIRRTDSDASEAAM
                     KYFVMGAIASGMLLYGISLVYGATGKLDLLDVANAVAVNWQQQNTLFAFAMVFILAGV
                     GFKLAAVPFHMWAPDVYQGAPSSVTLFISTAPKIAALGMALRFLTIGLVDITMQWQQI
                     ILVMALLSTGIGNLLAVIQTRIKRLLAYSAISHIGYALFGVLAASSAGYAAALYYILI
                     YAVMSAAAFGLVVLMSNHGMEIDSIDDLKGLNKRNPWLAFMMLLVMFSMAGVPPTVGF
                     FTKLLVLKALVDVHMTWLAVVGLLFTVIGAYYYLRIVKIMYFDQPENEAIVKIGTGNT
                     IVYSLNCLSLLYLGIFPGALIAACINAFAN"
     gene            17440..17516
                     /locus_tag="AC06IO_000943"
     tRNA            17440..17516
                     /locus_tag="AC06IO_000943"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17474..17476,aa:Met,seq:cat)
     gene            17630..18073
                     /gene="rimP"
                     /locus_tag="AC06IO_000944"
     CDS             17630..18073
                     /gene="rimP"
                     /locus_tag="AC06IO_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041173832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIO9874152.1"
                     /translation="MINDDLIVLLEPIIKNMGYELWGCEYLSQGKHSLLRIYIDKPDG
                     IGIDDCQEVSKQVSAMLDVEDPIPGHYSLEISSPGIPRPLFSIWQYQRYLGYEIHVKT
                     FKPVNGKRKLSGIIVSASEDTIVLDINNEHQEILLSNIVKANLTV"
     gene            18085..19563
                     /gene="nusA"
                     /locus_tag="AC06IO_000945"
     CDS             18085..19563
                     /gene="nusA"
                     /locus_tag="AC06IO_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIO9874153.1"
                     /translation="MSKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIG
                     VRIKIDSKTGDYETFRYWDVVNEEELEFPDRQLSLEQAKERKPSAAVGDRIEEIIPSI
                     EFGRIEAQTARQVIMQKVREAERELIIDQFRSKLGQLIYGTVKKVTRDNIIIDLGGKA
                     EAFLPRSEMLPHEMFRPNDRVRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEI
                     GENIIDIKAAARDPGNRAKIAVKTNDGRIDPIGACVGMRGARVQAVSSELGGERVDII
                     LWDDNPAQLVINAMAPADITSIVVDEDTHTMDLAVVKEQLSQAIGRNGQNVRLASQLT
                     GWTLNVMTTEEFESKNQEESSKIVNLFTSALEIDEEIATLLVAHGFSSLEEVAYVPKE
                     ELLAIEEFDEEIVEELRNRANDKLLEMALSSGKGLSGTPDDSLINMEGMTEDLANKLA
                     SKGITTMEDLAEQSVDELMEIEGISEEKAGALIMKAREPWFQ"
     gene            19652..22258
                     /gene="infB"
                     /locus_tag="AC06IO_000946"
     CDS             19652..22258
                     /gene="infB"
                     /locus_tag="AC06IO_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIO9874154.1"
                     /translation="MADVTVKQLAQVVGIPVERLLNQLQEAGLSFTDDQQTVNEEQKR
                     ILLNHLKGSSNRDISAAPERITLRRKSMSQVTVGHDMHSGKTVNIEVRKKKTFIKRSA
                     IPEQAEVEEPVVPPVVEEPVHEEITVVSEVSAETPELKETEEHPVIETVAELNESVKE
                     EEKINSEENTAESQDELTHANTDVIENLVDVVEEAIPVSKKEEVKPEKVSKKKHLEQT
                     DSDISEFKKGKKKPKYHTFEHDEEEQELHRRGGRSKFKKKKGTEKSDKYREAEETLTH
                     GFALPTAPIVREVLIPETITVAELAKRMSVKAAEVIKVMMSLGAMATINQVIDQETSV
                     IVVEEMGHKPVIIKEDAVETGLGEAISKGTKTEGRAPVVTIMGHVDHGKTSLLDYIRR
                     TKVAAGEAGGITQHIGAYHVSTPKGDITFLDTPGHAAFTAMRARGAQATDIVILIVAA
                     DDGVKPQTIEAIQHAKAAKVPIIVAINKMDKPDADPERVMNELSVQEVIPEAWGGDTM
                     FVNISAKSGMGIDDLLDAILLQSEVLELKAVTDGAAKGVVIESRLDKGRGPVATVLVQ
                     SGTLHKGDILLAGFQYGRVRALVSDNGDLVDSAGPSIPVEVLGLSAIPHAGDEAVVVP
                     DEKKAREVALFRQGRFRDVKLARRQKTTIEGIMENMTATESKVLNIVLKADVQGSLEA
                     ISDALTKLSTDEVKVEVISSGVGGITESDVHLAIASNAILIGFNVRADGTAKRLAEQE
                     SVSIHYYSVIYDIVDQIKGALTGMLAPQFKEEIVGIAEVRDVFKSPKIGAIAGCMVIE
                     GVVKRNNPIRVLRSNVVIYEGTLESLRRFKDDVLEVRQGFECGIGVKNYNDVKPGDLI
                     EVFETVEIKRDL"
     gene            22262..22633
                     /gene="rbfA"
                     /locus_tag="AC06IO_000947"
     CDS             22262..22633
                     /gene="rbfA"
                     /locus_tag="AC06IO_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIO9874155.1"
                     /translation="MSNNFKRTDRIAEMIQRKLALIIPQEIKDPRLKGFVTISAVKVA
                     ADLGHAKIYFTVLNEDKSVVTNILNGAASYLRSALARSITLRTVPQLHFIYDESIEYG
                     QRLSRLIDEVNPPDSSSDDNN"
     gene            22620..23531
                     /gene="truB"
                     /locus_tag="AC06IO_000948"
     CDS             22620..23531
                     /gene="truB"
                     /locus_tag="AC06IO_000948"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIO9874156.1"
                     /translation="MTTIESKCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTG
                     SLDPLATGMLPLCFGEATKICQYLLNADKSYETIGRLGSKTNTADCTGEVIFCIENYI
                     VSHEEMIATLEKYKGKTKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKL
                     EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNQMYTLDE
                     LQDMPLSQRFACLIPIDQAVQHLTPVILSDSEVTAIRQGKVISNKTSAVEGEDLRLYG
                     EQSQFIGIGQALIHGDIKAKRLVSFAL"
     gene            23679..23954
                     /gene="rpsO"
                     /locus_tag="AC06IO_000949"
     CDS             23679..23954
                     /gene="rpsO"
                     /locus_tag="AC06IO_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIO9874157.1"
                     /translation="MSLNSAEKAEIINEYKRGDKDTGSPEVQVSLITSRIKYLTDHFK
                     ENKKDFHSRRGLQELVNKRRKLLKYLKRNDQDRYQTLIQNLGLRDSY"
     gene            24069..26258
                     /gene="pnp"
                     /locus_tag="AC06IO_000950"
     CDS             24069..26258
                     /gene="pnp"
                     /locus_tag="AC06IO_000950"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIO9874158.1"
                     /translation="MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVV
                     WKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLF
                     PDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
                     LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSI
                     EELARDVGKSKPEWKAPEIDTVLKARINDVARNEVEAAYLIKDKQQRYQRLGELREQT
                     ISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDHRTVRPISVRTKFLE
                     RTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQV
                     GSPKRREIGHGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALM
                     DAGVPLKAPVAGVAMGLIKEGDRYAVLTDILGDEDHLGDMDFKVAGTEKGITALQMDI
                     KISGITNEIMERALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVAEDKIRT
                     IIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPDKMALEEAQKQIKALIAEIEVGQTY
                     QGKVSKIVDFGAFINLLPGKDGLLHISQICADRTQKVEEVLQEGQEIEVFVAGIDKQG
                     RVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINSGNKISEEEE"
     gene            26393..27484
                     /locus_tag="AC06IO_000951"
     CDS             26393..27484
                     /locus_tag="AC06IO_000951"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIO9874159.1"
                     /translation="MKLFSFFLVLISSASLGQGLPLHLLKYPSGFSVSIYAAPVSDAR
                     EMALGSKDIVFVGSRSAGKVYAVIPDKKNSYGTRVLTIASGLNMPNGVAFHKGALYVA
                     EIGRILRFDNIESNLNNPPEPVVVTTKLPKEKAHGWRFISFGPDDKLYIGIGAPCNVC
                     LMEDPRYATIMRMNADGQDLEIYAKGIRNTVGFDWDPFHHNLWFTDNGRDWLGDDLPP
                     DELNAAPRKGMDFGFPYCYGKSTADPVYGKTHSCSEFTPAVFEFPAHVAPLGMSFYTG
                     KLFPSNYWGQIFISEHGSWNRSHKIGYQITTVKIKNNHVTEVKPFISGWLQGEKVWGR
                     PVDTLVMPDGSLLISDDYANVIYRVGYQQ"
     gene            complement(27543..28082)
                     /gene="folE"
                     /locus_tag="AC06IO_000952"
     CDS             complement(27543..28082)
                     /gene="folE"
                     /locus_tag="AC06IO_000952"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIO9874160.1"
                     /translation="MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLD
                     EIINGALFDSDMSEMVIVKDIELYSLCEHHLLPFLGKCHVGYLPDGKVIGLSKIARIV
                     DFYARRLQIQERLTGEIAHCIESTTGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVM
                     LGEMRNNSSCRLEFLNLIR"
     gene            complement(28094..28435)
                     /locus_tag="AC06IO_000953"
     CDS             complement(28094..28435)
                     /locus_tag="AC06IO_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIO9874161.1"
                     /translation="MNCLFCKIAQGEIPATVVFEDKNILAFRDIRPQAPTHLLIIPKK
                     HIATINDVNDDDSELLANILIRAKKLAQAEGLSEMGYRLVFNVNSGGGQEVYHIHLHL
                     LGGRQMTWPPG"
     gene            28565..29101
                     /gene="ppa"
                     /locus_tag="AC06IO_000954"
     CDS             28565..29101
                     /gene="ppa"
                     /locus_tag="AC06IO_000954"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIO9874162.1"
                     /translation="MSLMEIPSGRDVPNEVNVIIEIPMHGEPVKYEVDKKTGALFVDR
                     FMTTAMFYPTNYGYIPNTLSEDGDPVDVLVITPVPLISGAVISCRAVGMLKMTDESGV
                     DAKILAVPTTKLSKMYQSMQTYQDIPQHLLLSIEHFFKHYKDLEEGKWVKVEGWVGPD
                     AAREEITSSINRYNHIKK"
     gene            29251..30516
                     /locus_tag="AC06IO_000955"
     CDS             29251..30516
                     /locus_tag="AC06IO_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIO9874163.1"
                     /translation="MQLPLSTLLVLLFFLICLSAFFSGSEIGMMSINRYKLRHLVKSN
                     DKQAMRVNSMLARPDKLLSVVLIGNTLANIVASTLATLIGQRFYGDAGVAIATALLTI
                     LILVFAEMTPKTLAAIYPQQVAFASSLPLSILQWIFSPLVHLISLITNGILRVFNISV
                     KRMQKEMLTGEELRSVVHEAGGLMPVEHKSMLISLLDLEQATVEDIMVPKADIVGIDL
                     DLPWIELLEQLETAQHTRLPIYHGSIDHLAGMIHVRNILNLALENKLDMENLLNSAEV
                     PYFIPEATPLNIQILNFRKMKRRSCFVVDEYGDILGLVTMEDILEEIVGEFTTDIAAL
                     SRDITPQQDGSVIVDASITIRHLNRMMGWSLPLIGPRTLSGLIIEHLGYIPPPDSCLV
                     IDHYHIEILRVSDNMIRGVKMIKTNKKRK"
BASE COUNT         9014 a         5232 c         6825 g         9492 t
ORIGIN      
        1 cctcttcatt tgaaggagtt tctggtatcg ctttaatcaa cagttcttgc tgtttcacaa
       61 gttcatgatg tttggttttt agctctcttt gttgagctaa taattgcagg aagctaactg
      121 aattcttaag tttttgctga ttatcagaat aattagaatc cgaataccat gaaagccagg
      181 aagatgggtt aaaattagat aataatccat caatctggtt ttggtattct tgtatcaatc
      241 cttgagtgtc ttcactttct tgaaatttcc tggttaactc tgcttttaaa tcgggtgcca
      301 gggagtggtt agttactacg gtttcaatac gacccaggtc tttaagcaat agttcaattt
      361 gtttttctag ctcattctct tttttattaa tatcagttgc agataattgt ttggcgaatc
      421 gcttttgcaa ttcatcccat ttttcacaag cgcgagttaa ttttttaatt tccaattcaa
      481 ctgcagctat aaatttttgt aaactatcaa cagcatcttc ttccacagga ggttgaatgc
      541 cgaactgaga caaatctgcc attaaggcat tccatttatt tttcagcaaa accagcttta
      601 atttggcacg tgtagtttct atttccttac tggttttgaa gagataactg tagtgcccga
      661 ctacaattga tttttgaacc tctccgtatt cagggaattt ataaggaggc gctaatgggt
      721 tttgtttgga caggacttca ttcgctttag gttcgatttc tttgtataaa ggatttaatt
      781 cattttttgc gtgctcaatt tttttagcca aatcagaaat ttttctttgc agttcttctc
      841 cttttttcat gacctcatct aatttgtgtt ttttcctttt atactcttct tgttcttcag
      901 ctaaactgga atattcatca acatattttt taattaacaa taaaagattt aattgggatt
      961 caacttcaac aggagctttt ccttccggca gtagatttga actaaatatt tgctttaaat
     1021 ttaaggggtt aattggtgga gtcaaggtgg agatttcatt cattaagtgc agtatgattt
     1081 cgttcattat aaaaccctcc tggttaaatc atagtaggat tctatcgaaa tcaaaccact
     1141 aagcaagtag aaaaatgtat tttttgcaaa aaaaatgaat aattaattct gaaaaaaaac
     1201 atagataaag ctacacatct gagtgaagtt tcggtatatt gcacagctgc acactatcag
     1261 ttgtttactg ataggctgga aataatagaa aggattttgt ccgcctatac tagaaattat
     1321 tggagggagt gtgaggcaaa agatagttgc tggtaactgg aaaatgaatg gccagattca
     1381 acaggtgact gaattggttt cgcaaattga ggaattaatt ggctttgatt gcgcagcaca
     1441 ggttgctgta atgcctccca gcatttatat acccaaggta agagactgct taaggacggg
     1501 gaagattgta gtaggtgcgc aaaatgttta tccgaaagat tatggtgcct acacaggtga
     1561 actgtcagct cccatgctta aagattttga ttgccgatat gttctggtgg ggcactcgga
     1621 gcgcaggcaa ttttttcatg aagatgaaaa ttttgtggcg caaaaattcc accatgtcaa
     1681 agatcatggt atgatacctg ttctttgtgt tggtgaaact ctttctgaaa gagagaatgg
     1741 aaaaacagag caggttattg ctcaacaggt gctcgcagtg agtgcaaaag ggaaagattg
     1801 ttttcgtgat tgcgtagtgg cttatgagcc tgtatgggca attgggacag gaaaaactgc
     1861 tacacctgaa caggcacaaa aaatacacca gtttattaga gatctggttg gagaaataaa
     1921 tgatagcgat gcgaaacatt tgacgctcat atatggtggc agtgttaatg aaaataatgc
     1981 aaaagcttta ttttccatgc cagatattga tggagggtta gtgggtgggg catcgttgaa
     2041 tgcaaaacaa tttgtggaaa ttgtgaaatg tatcaattga tattaatgat tcatgtctta
     2101 attgctgtaa ttttaatagg cttagttctt atccaacatg gtaagggtgc tgatattggt
     2161 gctgcttttg gttctggcgc atcaaacacg ttatttggaa gtcaaggtac tggtggcttc
     2221 ttgttcaagt taactggcgg tctggcaatg acttttttta ttaccagttt gatgctgtct
     2281 tatatggttt ctacgcagaa ccaaaaggca ggacagttag ctattcctca acaaaccggt
     2341 gctccagtca gttcagtccc agtgccagtt gaaaaaaata ataagaagta actaaaaaga
     2401 tgtagtgtct ttagggctgt tgtcttttgt gatgaactca ggataacaaa aataaaaata
     2461 agccgaagtg gtgaaattgg tagacacgcc gtcttgaggg ggcggtggcg caagccgtgc
     2521 cggttcgagt ccggccttcg gcaccatttt aaatcgagca aggtgaactg atgctatctc
     2581 aatatctacc cgttctcgtt tttataattg ttgccttgat tttaggtgca gtaatgatag
     2641 gcgctggttc tttgctttcc aagcatagtc cggatagcgc taaaaactcc ccctatgagt
     2701 gtggttttgg cgcctttgaa agctcccata ttccgtttga tgtacggttt tatctggtgg
     2761 ctattttatt tattattttt gatctggaaa cagcattctt ttttccttgg gccttggcat
     2821 tacgtaaaat aggatggttt ggcttttatg ctatgatgtt atttcttggg ctgttggtta
     2881 taggttttat ttatgaatgg aaaagaggcg cccttgagtg ggaataaggt tctttcattc
     2941 ttttaaaata tttgatttaa tctttcgttt tagccattac tatgggaagc aaaggacgct
     3001 tggcaaatta ttacagagct ttttgctttt agatgaacct gatggatttt taggttggtt
     3061 tttaaaactt ggtcatcctt tgctaaaacg atagctgaga ttatatttta agccattgta
     3121 ataatggtta tgtaaaactg gattattaat tatttaactg cttagaggcg aactatggcg
     3181 gttgcagagc tagaaaaaaa aggttttgag ttgactacgg tcgaaaaatt ggttggttgg
     3241 gcacgcagtg gctccatgtg gccaatgaca tttggcttgg cttgctgtgc cgtggaaatg
     3301 atgcacgtgg gagcagcacg ctatgatttg gacaggtttg gtattatttt ccgccccagt
     3361 ccacgccagt cagatgtcat gattgtagca ggcactttat gtaacaaaat ggccccggca
     3421 ctcagaaaag tttatgatca gatgccagaa ccacgctggg ttatttcaat ggggtcttgt
     3481 gccaatggtg ggggatatta tcattattcc tattcagtgg ttcgtggctg cgatcgtatt
     3541 gtgccagtcg atgtctatgt tcctggctgt ccaccaacag cagaagcgtt gttatatggc
     3601 attattcaat tacagaataa aatcagacgg aaacctgttt tagaggcata agctcattaa
     3661 gggtgcagat tagatgacaa aaaatgagta tttgattgaa aagctacaag ttgatttagc
     3721 aaatcatatt accgagttaa cctccgcata tggtgaagta actattgagt gtgaggtgca
     3781 aaatttattg cctgttatga ttgagttgag agacagagag gaattttctt tcgatgagtt
     3841 aattgatctc tgtggcgtag attatttgca ttatggtgat tatgattggg aaacagagtc
     3901 tgcgacagaa catggatttt cacgaggtgt cgagcgtcag gaagccaagg cttatgctgt
     3961 aaataaacca cgattcgccg tagtctatca tttattatca accaaaaaaa atcatcgttt
     4021 acgagtcaaa ttatttgtag aagaatccca tttaattgtt ccatcagtac atcatttatg
     4081 gaagtctgct aactggtttg agcgggaagc ttatgattta tatgggattt tattcgatgg
     4141 ccatcctgat ttaagacggt tactgacgga ttatggtttt ataggccacc ctttccgcaa
     4201 ggattttcca ctcagtggag aggtggaaat gagatatgat gcaaaactgc aaaaggtaat
     4261 atatgcccct gtagacattg tgccaagaat tgtagttcca aaggttattc gtaacgataa
     4321 ccgctacata ggtaatgaag gtagcaaaaa tgattgaact taaaaattat accttgaatt
     4381 ttggcccaca acatcctgct gcacatggtg ttttaagatt ggttcttgag ttagaaggtg
     4441 aaactatcgt tcgtgctgat ccacatattg gattgcttca tcgtgcaacg gaaaaattgg
     4501 ctgagaccaa gccttacatt caaagcattg gttacatgga cagattagat tacgtctcaa
     4561 tgatgtgcaa tgaacatgct tatgtcatgg cgattgagaa gttattgggt atagaaccgc
     4621 ctttgcgagc caagtatata cgaactatgt ttgatgaggt gactcgaatt ctgaaccatc
     4681 tcttgtggtt aggagctact gcattggata ttggtgcaat gacagtgttt ctttattgtt
     4741 tcagagagag agaagatttg tttgattgct acgaggcagt ttcaggagca cgtatgcatg
     4801 ctacctacta taggccagga ggggtagcaa gagacttacc agatactatg cctcaataca
     4861 aaccgtcaag atggcatagc gaaagagaga ttgaaaaaaa gaatcacaat cgtcagggca
     4921 gtcttttgga ttttctttgg gactttactg aacgatttcc ccactgtgtg gatgaatacg
     4981 aaacattgtt gaccgataat cgtatatgga agcaacgtac tgtagatatt ggtgtcgttt
     5041 cacctgaaaa tgcactgcaa tggggattta ccggaccaat gttaagaggc tccggtattg
     5101 cctgggattt acgcaagaaa caaagctatg ctgcttacga cagggttgaa tttgatattc
     5161 ctgttggcaa gacaggggat tgttatgatc gatacttggt aagagtcgag gaattgagac
     5221 aatccaatcg aattataagg caatgtattg agtggttgcg caagcatcct ggtccagtaa
     5281 aagtcgatga ttataaagtg tcgcctccac gtcgtgtagt aatgaaacat gacatggaag
     5341 cattaattca tcactttaaa ttatttaccg aaggcttttg tttaccgcga ggtgaagttt
     5401 attcatctgt tgaggcgcca aagggcgagt ttggtatcta tatggtgtca gatggagcta
     5461 ataaacctta ccgtctgaaa ataagagctc caggctttgc tcatttatca agctttgatg
     5521 atatggttag aggtcacatg ctagctgatg gagtggctat attggcaagt caagatatag
     5581 tgtttggaga gattgatcgt taatttaacg acatgcttgc ttaaagtgta ttaagagata
     5641 ataaacgata taaaggtttc gaaatgtctg ctaattcagc aaaaatatta aatcaattga
     5701 tgccggcaaa ccgtatgagc gatattgatc attggatatc aaaatatccc aaagatcaaa
     5761 aacaatccgc ggtcatgagt gctcttcgaa tagtacaaga agaacatggt catttaacaa
     5821 cggaattaat gaatgcagta gctgaatatc ttgatatgcc gcccatcgca gtttatgaag
     5881 tagcaagttt ttatacaatg tatgaacaca agccagtggg gcgacattta attaatgttt
     5941 gtaccaatat ttcttgcatg ctgagggatt ctgctggcgt tgttgaacat ttagaaaaaa
     6001 aattgggtgt taatctaggt ggaacaacag aagatggtcg atttacttta agatcagttg
     6061 aatgcttggg tgcttgtgtg aatgcgccaa tgatgcaagt tgataaagat tatcacgaaa
     6121 atttgactgc tgaatctata gataaagtat tggaacagta ccaatagaaa ataaaggtgt
     6181 ttgccatggt tgaactaaat caagtttgtt accgaacatt tcatttacca gagccctggt
     6241 cgctatcaac ttatgaaagt attggtggct atagcgcatg gcgaaaaatt ttaaaagagc
     6301 aaacatcacc tcaattaata attgaagagt tgaaaacttc tgccttgcgc ggacgaggag
     6361 gggctggatt cccaaccggt ttaaaatgga gttttatgaa tagaaactcc cctgttcaaa
     6421 aatacgtagt atgtaattct gatgaaggtg aaccgggtac ttgtaaggac agagagattc
     6481 ttactttaaa tcctcaccaa ttaatcgaag gaatggccat agctgggtat gttatgggcg
     6541 ccacggttgg ttacaactac ataagaggag aattctggct accttttgag cgtactgagg
     6601 ctgcaataaa agaagcatac gatgctggct tgctaggtaa aaatatatta ggctccggcg
     6661 ttgattttga tttatataat catttaggag cgggagctta catttgcggt gaagaaactg
     6721 cattgcttaa ctcgattgaa ggaaaaaagg gaatgcctcg ttttaaacca ccattcccgg
     6781 caaattatgg cttgtatggt aaaccgacaa ccataaataa cacggaaaca tttgcgtctg
     6841 ttccggttat tatggaaaaa ggtggagaat ggtttttaaa attaggtaaa ccgaacaatg
     6901 gcggtgccaa atgctttagt gttagcggcc acgttaacaa acctgggaac tatgaaatcc
     6961 ccttgggcac gccattcaaa acgctgcttg atctggctgg cggcgtcatt aaaggccgaa
     7021 aaatcaaagc cgtgattcct ggtggttcct ctatgccagt gctcccaggt gacgtcatgc
     7081 tggggctgga tatggattat gacagtatac agaaagcagg ctcaggcttg ggttcaggcg
     7141 cagtcattat catggatgaa accacctgca tggtggatgc tttgtatcgt atttctgagt
     7201 tctacatgga tgaatcctgt ggacaatgca caccttgtcg agaaggcaca ggctggttag
     7261 tcaggttatt acacagaatt ataaacggtc atggcgagcc gggagacgtg gagaagttag
     7321 tgaatgtggc gaataacata gagggaagaa ccatttgtgc attaggcgag gcagcagctt
     7381 ggccagtaca gagttttgtt aaacatttct atgatgaatt tgaatatttc ataaagcata
     7441 aacgctcgat cgtcgcagca taattaagaa ggttagacca tggctactat tgaaatcgac
     7501 ggtaaaacat ttgaagcaga aaacggtaaa atgattatag aggttgctga tgaggcgggc
     7561 atctatattc cacgtttttg ctatcataaa aaattatctg tggcagctaa ttgccgcatg
     7621 tgtttggtcg aagtggaaaa tgggagaaaa ccggttcctg cttgtgctac acctataact
     7681 aatgggatga aagtatttac aaagtcagag caggctatac actcccaaaa ggtagtgatg
     7741 gaatttctct tgataaatca tcctttggat tgccctatct gtgatcaggg tggagaatgt
     7801 gagcttcaag atatttctat ggggtttggc gctgataaat cagagtatac agaaagcaag
     7861 cgtgcggtag acgatgagaa tttaggaacg ctaatatcta ctgaaatgac acgttgcatt
     7921 cattgcacac gctgtgtacg cttcggcgag gaaattgcag gggtaagaga gctgggagca
     7981 accggccgtg gcgaaaagac gcagattggt acttatgtag aacacagtat gacatcggag
     8041 gtttcaggga atatcataga cttgtgccct gttggtgctt taacttctaa gccttatcgg
     8101 tttaaagccc gggcatggga attgactcaa aacgacagtg ttgcgcctca tgattgcctg
     8161 gggtcaaatg ttcatattca tacaagaaat aataaattaa tgcgtgtagt tcccagggag
     8221 aacgaatcta tcaacgaaac ctggctttca gacagagata gatttagcta tttggggtta
     8281 aatgcggctt caagggcgag caaaccacaa atcaaaaaaa atggccaatg ggaaccggtt
     8341 gattgggaaa ccgcacttaa gtttgcggca gaagggataa gccgagttat caaacaacat
     8401 ggcccagagc aaatggccgc ttttgcgtcc agttcttcta ccctggaaga aatgtattta
     8461 ctacaaaaat taatgagaga gctaggtgtt caaaatctag atcaccggtt acaacaaatt
     8521 gattttagag atcaggcatt tttacctact acgccagtaa gcactttgcc ctacgcagag
     8581 atagatcatc agaattcaat tctgttaata ggatgtaata ttcatagaga agtaccgttg
     8641 gcaggaacaa gggtaagaaa agcttttcgc aatggcgcaa aaatttatgc ccttaatcca
     8701 gtagattttg attatcattt tgatttgtcc ggacgagtca ttatttctcc tttggaaatg
     8761 ccgatgcaat tagctaaatt agccttagcg cttacaaccg aattgacatc gcttccagaa
     8821 gaagttcaaa aactgttaat aggtttggaa gtagacaaac aaaccaaaca aatagcacaa
     8881 tcattaaaag aagagaaagc ctgtttgata accggagcaa tagttgaaaa tcatcctgaa
     8941 gcttccttat tgcgtacttt ggtcgctatt gttcaaaaac ttagtggagc caaattagtc
     9001 agattaacga ctggagcaaa cagcgcgggc gcatgtattg caggtatgct gccgcataga
     9061 acagttgctg ggaaatcaat tgcagagccg ggtttcaatg tacaagaagc gttaaattcc
     9121 aagttaaaag gttatttact catgggtgtt gaaccaggct atgattttgc aaatccagct
     9181 ggggcaaggc aatcaatgtt agctgcagag tttgttgtac tcttatcagc ctatgagcat
     9241 gaatccatgc atgattatgc agatgttatt ttaccgatcg ctccttatgc tgaaacatct
     9301 ggaacataca taaacattga taatacctgg caaacagtaa aaggagccat gctgccattt
     9361 ggcgaatcac gtccagcctg gaaggtgctt cgtgttttgg gtaatttatt gcactgcaaa
     9421 aaatttgatt atacctcaac agaggacatc ttggaggaag tcaaagaagc agtatcgatg
     9481 acaatggagc acgaatacga accttactac cctgaatcat tgcctgtcat taatcaatca
     9541 ctggtaagag ttggagagtg gcctctttat cgaatagatg ctattactag aaatgcaaaa
     9601 gagttacaat tatgtgccgc atcagaatct gcctgtataa gaattcatcc atcaactgca
     9661 gatagattga aattagaaga aattgctact gtatctcaag gagatattga gataacattg
     9721 ccgctaaaac gtgatgagcg cattgcggtt gatgttgtgt gggtggcaaa tgcaatgcca
     9781 gaaacagttg accttggtca ctcgtttgct gcaataacaa taaagcgcta gaaaggtata
     9841 aaaatgcttc atactattgg catactaatt tggattatta ttaaaattct tgtgatagtt
     9901 gtccctttac tgatttccgt ggcctaccta acctatgcgg agcgtaaagt gattggctat
     9961 attcaagtgc gtatcggccc gaacagggtt ggtttaaagg gtttgctaca gccatttgct
    10021 gatttattga aattaataac taaagaaatc atagttccca ctcgatcaaa caaatacctt
    10081 tttgttattg ctccactttt tgctttagtc ccctccttag tcggttgggc ggtaatccct
    10141 tttcaggaag gtattgtcct ggcaaatatt aacgcaggag tcttatactt atttgccatg
    10201 agttcgttgg gcgtatatgg tgttcttatt gcaggatggg catcgaattc caaatatgcc
    10261 atgtttggcg cactgcgtag tactgcccaa acagtctctt acgaaattgc tatgggattt
    10321 gctcttgttg gtgtgctctt ggccgctgga agcatgaact tgactgatat tgttaattct
    10381 caaaaaggtg gaatgctgca ttggtggttt attcccttgc tccccctatt cttggttttc
    10441 tggatctccg gtattgcaga aactaacagg gctccttttg acttggcaga aggtgaatca
    10501 gaaattgttg ctggcttcca tgtagaatat tctggcattg gattcgcttt attctttctc
    10561 tctgaatacg ctagtatgat tttgatttca acagttttgg ctattttatt catgggcgga
    10621 tggctgtcac cttttgaagg tattacgttc ctggatcaaa tcttttttat agtacccgga
    10681 tttgtttggt tgcttctgaa aattagtttc ttcctttttg tgtatttgtg ggtaagggct
    10741 actttccccc gatatcgtta tgaccagctt atgcgtttag gctggaaggt acttatccct
    10801 gttactatag tttggttaat agtaaccgcc gttatggtgg ttgctcatgt caagccatgg
    10861 ttttgacggt tagaagagaa gccgatgaaa aaagtatatc attatataat tcactatgtt
    10921 cgtacctact tactgttaga gctattagcc ggtttatggt taacagtaaa gtattttttc
    10981 aggaaaaaaa taactgttca gtttcctgaa gaacaaacgc cactttctcc aagattcagg
    11041 gggcttttgg cattacgtcg ctatcccaat ggtgaagagc gctgcatcgc atgtaaatta
    11101 tgtgaggccg tttgccctgc attagctatt actattgaat cagagcaaag agacgatgga
    11161 tcacgccgta ctactcggta tgatattgat atgtttaaat gcattaattg tggattatgt
    11221 gaagaatctt gccctgttga ttcgatcgtt gtcactccaa ttcatcacta ccatatcagc
    11281 gaacgtggtc aaaatattat gacaaaagag aagcttttag cagtaggcga tttgatggaa
    11341 tcacaactcg cagcagacag agctgctgat gaaaaatacc gttgatgggg tgatttatgc
    11401 atgaatgggt aatccaagca attttttatg tttttgcatt attgaccgta ctgtcggcag
    11461 taatggtaat aagccaaaac aatccagttc gttgtgtttt gtttttagta ctcacttttt
    11521 ttaccagtgc ggttctatgg attttagctg aagctgaatt tttagcatta atccttatcc
    11581 tggtatatgt tggcgctgtc atgactttat ttctgtttgt cgtcatgatg ttaaacattg
    11641 acattgaaag catgaaaagc catttaatta aatatctacc atttggatta ataatagttg
    11701 ccttattgac tgggttatta atggtagcta tccctaaaga tttatttaag aacagtgtgc
    11761 aaactcaaga aaagcctttg agtgttaact cacaattaat ggatttatct accgaagatg
    11821 ggctggttac acctaaaaat gctacatcga acacagaaaa gttaggaatg gtcctttata
    11881 cagattatat tcttgccttt gaaatagctg ccgtcatttt actagtggct attgtttctg
    11941 caattacctt ggtgcatagg ggtgctatca ggtcaaaacg acaagatatc actcagcaaa
    12001 ttatgactcg aagggcagaa cgagtgaagt tgatcagtat gaagtcagag aaatagttat
    12061 tggggaattc tttatgatac ctgtttatga ttatttagta ttaggagtga ttttgttcgg
    12121 cttgagtcta gttggtatta tgctcaatcg taaaaatatc atcttattac ttgtttgtgt
    12181 tgagttgatg ctcttggccg tgaataccaa ttttattgca ttttctcatt actacaatga
    12241 agtaggtgga caggtatttg tctttttcat tttaactgta gctgcagcgg aagcagcaat
    12301 aggtttggcc atagtcatgt tgctttatag aaatagaggc aatattgacg ttgataaaat
    12361 gaatcattta aaaggttaaa atcgtgagta ttcaacaagt ctgcttagta atcgttttga
    12421 ctccccttgt tggttctgct atagcaggat tttttagaaa ccagattgga cgagtcggag
    12481 ctcatactgt aactatttta ggcgtagcta tttccttagc ctgttcaatt tttcttgcgt
    12541 ggggcctgtt ttctggttca atcccaaaca ctaatagtct tgtttaccac tgggctagcg
    12601 ggggggcgtt aattccctac gaatttaata ttggtttttt aatagaccct ctaagcgttg
    12661 tgatgctggt tatagttaat tttgtttctt tgttagtcca tatctacagt attgggtaca
    12721 tggcagatga tgatggttat cagcgatttt ttagttatat ctctttattt actttcatga
    12781 tgctcatgct agttagcgca aacaactttc tgcagttatt ttttggctgg gaaggggttg
    12841 ggcttgtttc ttatttatta atcggatttt ggtatcaaaa ggaatctgca atagaaggca
    12901 gcctaaaagc atttcttgta aaccgtgtag gagattttgg atttgtatta ggaataggtt
    12961 taatttttgc ttatgctgga agccttgatt atgaaaaagt atttaccagc gcaaattatt
    13021 tagcaagcca gaacatagag ttatttagcg gctactcctg gtccctgatt acagtgattt
    13081 gcctgctgct ttttgttggc gccatgggca aatcagcaca ggtgccactt cacgtttggt
    13141 tacctgagtc aatggagggt ccaaccccta tttccgcgct aatccatgcg gcgacaatgg
    13201 tgacagcggg tgttttcatg attgctcgca tctcaccttt aattgaattg tctacagctg
    13261 cgcttagcac agtattagta attggtgcta caggagcatt gtttacagga atactggctt
    13321 tggttatgaa cgacataaag cgcgtggtag catactctac tttatcacaa ctaggctata
    13381 tgatggtggc aatgggtgcc tcagcctata gtgccggtat gtttcattta ttgacgcatg
    13441 cttgctttaa agcgctattg ttccttggtg ctggttcagt cattattggc atgcatcatg
    13501 aacaagatat gcggaaaatg ggtgggttgt ggaataaaat gccgattacc tatgtaacct
    13561 atgtcatagg cagtttggca ttgtgtgcat ttcctccttt tgctggtttt tattcaaaag
    13621 ataccattat tgaagcagcg cagctttccc agatacccgg cagcagttat gcttattttt
    13681 gtgtaacagc aggtgctatg gttacagcat tatatacttt cagatcctta ttcatgacat
    13741 ttcatggtaa gccacgtatg gatgaacata cattaagtca tcttcatgaa tctccatggg
    13801 tagtatggtt accattagta ttgctcgcta tcccatcaat aattttaggc tatgtgcttt
    13861 acatgcctat tctatttgat acaccaagcc tcttaggctc atcaattttt gttttacctg
    13921 agcataatgt tttagccgaa cttgctcatg aagtaagttc gccatttgca agtgctgttc
    13981 acgcgattta ttctttgcca ttttggatta cagtactcgg agcagttatt gcctggattt
    14041 gttatatcgc tgtcccatct attcccggtt atttagctag gtatttttca attgtttatt
    14101 ctattttact caataaatac gggtttgatc gctttaacga gttgttcttt gtgaaagggg
    14161 ctagaggatt aggcattgct ttttacaaga ttggtgatca aaaactaatc gatggtgcag
    14221 ttgtcaatgg tagtggacgc ctggttcgct ggttctctgg gaaagggcga aagatacaaa
    14281 gtgggtacat ctatcattat gcgacagtaa tggtgtttgg attgctggcc tttttatgct
    14341 ggttaatcct ggactgaaga taaggtgaag gtatgcatta tttgctcaat ttattaattt
    14401 ggttaccgat attgggtggc gtttttgttt tgctgactgg ggatgataaa aatccaaatg
    14461 tatcgcgtta tttaacatta ttcaccatac ttctctgttt agtgctctgc atacctttag
    14521 ttaaaggatt tgatctgcaa tcttttggca tgcaatttat agaggaaatc ccctggatgc
    14581 cggcactggg tattaattat actcttggta ttgatgggtt atctttgcta ttaattgttt
    14641 tatccatttt tactaatttg atagtgattt tggctacatg ggatagcatt agtaaaaaag
    14701 tttcccaata tttatcagcc tttttaatta tgcaaggcct tctggtcggt atattttctg
    14761 ctatggatgc catagtattt tatattttct gggaagcaac cttgattcca atgtatctga
    14821 ttataggtat ttggggttct gataacagag tttatgctgc aataaaattt ttcctatata
    14881 cttttttagg gtcagttctc atgctggcca gtttcctgta catgggctac attgcaggtt
    14941 catttaagat cgaaaccttt tatgctatca aattaggcat gactgcacaa acattgattt
    15001 tcattgcatt tttcttaggt tttgcaataa aaataccgat gtttccagtc catacctggt
    15061 tgccagatgc gcatactgaa gctcccgctg gtggttctgt cgttttagca gcaattttgc
    15121 taaaactggg ggcctatgga tttatccgtt tttctttacc aatagtcccg gatgcttgca
    15181 gcaaatatgc tcctctgatg gttgccttat ctttgatcgc agtggtttat ataggactca
    15241 ttgcaattat tcagaaggat atgaaaaaat tgattgcata ttcttcaatt tcacatatgg
    15301 gttttgtaac tctaggctgt ttcgctatat ttgcaattgc tcgccatgga ggactaagca
    15361 acgccggttt aatccttgag ggtgcaattg tcgttatgat atcgcatgcc tttgtttcaa
    15421 gtgcaatgtt tgcaggtgta ggttttatat atgatcgtat gcatacaagg cagctcgcgg
    15481 attttggcgg aatagtaaat actatgccta tatttgcttc tttttttatg ctttttgcta
    15541 tggcgaatgc tggcttgcca ggaacatccg gatttgttgg tgagtttatg gtgatattag
    15601 gtagcataaa ggctggtttt tgggttgcat tttgggctgc aactacattg attattggag
    15661 cagcatatac gctatggatg tacaaaagag ttatttttgg tcctgtagct aataaaaagg
    15721 ttgctgcctt aaaagatata tcaggttttg aattaagtgc ttatgtattg cttgcaataa
    15781 tggtcattgc tctaggagtg tatcccaaac ccatgttaga gtttgtgcat caaactgtaa
    15841 gtcatacttt ggcattggct gataaatcta aactctaaca aagaactgcg ataggcttaa
    15901 attgcaatca agttccttag caaggttaaa tatgatgaca ttactagaaa atatccatgt
    15961 agcaatacca gaaatgatta ttttaataac cgcgtgtttg gcgttgcttt ctgatttgtt
    16021 ctttcgtcat aaattaaaat caattgcgtt ttacatatct tgtgtaggaa ttatagtttc
    16081 tgcagcagta agctttgtat ttataggaag ctacaagtta cttatattca acaaattatt
    16141 tataagtgat gatataagtc atttaatgaa actgtttatt gatatcacgg ttttattaag
    16201 ttttgtctat tcccaaaact atctggatga acggcaaatg cctacaggtg attattatgt
    16261 tctggggctg ttttcaacac taggtatgat gattcttgtt tcagcacact cgctgttgtc
    16321 cctttatcta ggtttggagt taatgtcatt gccgctctat gcaatgaccg ccatccgccg
    16381 aactgatagc gatgcatcag aagcggcaat gaaatatttt gttatgggtg ctattgcttc
    16441 tggtatgctg ttatatggaa tatccttggt gtatggtgca acaggaaaac ttgatttgtt
    16501 agatgttgct aatgcagtgg cagtcaattg gcagcaacaa aatactcttt ttgcgtttgc
    16561 aatggtattt atacttgccg gcgtaggatt caaactggct gctgtccctt tccatatgtg
    16621 ggctccagac gtatatcaag gggcaccaag ctctgtaaca ctttttatta gtacagcacc
    16681 caagattgct gcactgggga tggcactgcg atttttaact attggcttag tcgacatcac
    16741 catgcaatgg cagcaaatta ttttagtaat ggctcttctt tctaccggca taggtaattt
    16801 gttagcagtg attcaaacca gaatcaaacg cttgttagct tattctgcaa tttctcatat
    16861 aggatatgct ttatttggag ttttagccgc cagctctgct ggctatgccg cagcactgta
    16921 ttatatttta atctatgctg taatgtcagc ggctgctttt ggtttagttg tattgatgtc
    16981 aaatcatgga atggaaattg atagtattga tgacttaaag ggcttgaata aaagaaaccc
    17041 gtggctggcg tttatgatgc tattagtcat gttctcaatg gctggcgtgc cgccaacggt
    17101 tggcttcttt acaaaattat tggtattgaa ggcattagta gatgttcaca tgacttggtt
    17161 agctgttgtg ggtttgctat ttacagttat aggtgcctat tattatttgc gaattgttaa
    17221 aattatgtat tttgatcaac ctgaaaatga agctatagta aaaattggta caggcaatac
    17281 catagtttat tcacttaatt gcctttcctt actatattta ggtattttcc caggcgcttt
    17341 aattgcagcc tgtattaatg catttgcaaa ttaattaata aactttaaca aaagtacttg
    17401 cttagggacc caagtatcag tatacttgcc ctcagttgac gcggggtgga gcagcctggt
    17461 agctcgtcgg gctcataacc cgaaggtcgt aggttcaaat cctgcccccg ctaccaattt
    17521 tataaaaccc catttttggg gttttttatt atgttgacta ggatactatc taaatctaag
    17581 tcagttacca ggcttgggcg agtgcgccct ttttttgttt atacatacta tgataaatga
    17641 tgacttgata gtgctattag agcctataat caaaaatatg ggttatgaac tttggggctg
    17701 tgaatatctt tcccaaggga aacactcgtt attaagaatt tatatagaca agccagatgg
    17761 aataggaatc gatgactgtc aggaagtgag taagcaagtc agtgcaatgc ttgatgttga
    17821 agaccctatt ccagggcatt atagtttgga aatatcatct cctgggatac cccggccctt
    17881 gtttagtatc tggcaatatc agcgttacct tggttatgaa attcatgtaa aaacttttaa
    17941 gccagtcaat gggaagcgaa aattatcagg tattattgtt tcagcatcag aagatacaat
    18001 agtattggat attaataatg aacatcaaga aatactttta tcaaatattg tgaaagcaaa
    18061 tttgacagtc tagagaggcg aacaatgagc aaagaattgt tattagttgc tgaggcatta
    18121 tcaaacgaaa gaggcgttag taaagacgtc attatacttg ccattcaggc tgcactggaa
    18181 tctgcaactc gaaagattct tggtttagat ataggtgtgc gtatcaaaat agattctaaa
    18241 accggtgact atgaaacctt tagatactgg gatgtagtga atgaagagga attagagttt
    18301 ccggaccgcc aactgtcctt ggaacaagca aaagagcgaa agccctcggc tgctgttggt
    18361 gaccgaattg aagaaattat tccttccata gaatttggaa gaattgaagc gcaaacagcg
    18421 cgacaagtta ttatgcaaaa agtcagggaa gcggaacgcg aattgatcat agaccaattc
    18481 cgtagcaaac ttgggcagct aatctatgga actgttaaaa aagtaactcg tgacaatata
    18541 attattgatt taggtggtaa agcagaggct ttcttgccac gctctgaaat gttgcctcat
    18601 gaaatgtttc gccccaatga cagagttcga gcatatctgt acgaaatcac gccgcaggcg
    18661 agaggaccac aattgttcgt aagcagaact cgaaatgaaa tgctcattga attattccgt
    18721 attgaagttc cagaaattgg tgaaaatata atagatatca aagctgcagc gcgcgatccg
    18781 ggaaatagag ccaaaattgc tgttaaaacc aatgatgggc gtattgaccc catcggcgct
    18841 tgcgttggta tgcgtggagc aagggtacaa gcggtttcca gcgaattagg cggtgagcga
    18901 gttgatatca ttctatggga tgataaccct gcccaattag tcataaatgc gatggctcca
    18961 gctgatatta catctatcgt tgtggatgaa gatacccata ccatggattt agctgttgtg
    19021 aaagaacaat tatcgcaggc aattggtcga aacggacaaa atgttcgttt ggctagccaa
    19081 ttaactggtt ggacattgaa tgtcatgaca acggaagagt ttgaaagcaa gaatcaagag
    19141 gaatcaagta aaatagttaa tctgtttacc agcgcgctag aaatcgatga ggaaatagcg
    19201 acattattgg tagctcatgg attttcttct ctcgaagaag tggcttatgt tccgaaggag
    19261 gagctgctgg ctattgaaga atttgatgaa gagatagttg aagagttaag aaacagagca
    19321 aatgacaagt tattggagat ggcactgtca tcaggcaaag gattatctgg tactccagat
    19381 gattccctta tcaatatgga aggcatgaca gaggatttgg caaataaatt agcaagtaaa
    19441 ggaattacaa caatggaaga tcttgcagag caatctgtag atgaattaat ggaaatagaa
    19501 ggcattagtg aagaaaaagc aggtgcttta attatgaaag caagagaacc atggtttcaa
    19561 taaactatgg ttatcgaaat tgaacaatac gatgctagat gcattttaaa tatattcagc
    19621 tttataaaaa gttgctgaga ggggattaaa tatggcggat gtgacggtta aacaattagc
    19681 tcaagtcgtt ggtatcccgg ttgagcgctt attgaaccag ttacaggaag cagggttatc
    19741 ttttactgat gatcaacaga ctgtgaatga agaacaaaag aggatcttgc ttaatcattt
    19801 aaaaggtagc tctaatcgtg atattagtgc tgcgcctgaa agaataactt taagacgtaa
    19861 aagcatgtct caggtcactg ttgggcatga tatgcatagt ggcaaaacag ttaatattga
    19921 ggttagaaag aaaaaaactt tcatcaagag aagtgcaatt cccgaacaag cagaggtaga
    19981 agaacccgta gttccgcctg ttgttgaaga gcccgtccat gaagaaatca ctgtggttag
    20041 tgaagtgtct gcagaaactc ctgaattgaa agagaccgag gagcatccgg ttattgaaac
    20101 tgtagcagag ctcaatgaat cagtaaaaga agaagaaaaa atcaattcag aggaaaacac
    20161 cgcagagagt caggatgagt taacccatgc aaacactgat gtaatagaaa atcttgtgga
    20221 tgttgtcgag gaggccatcc ctgtttccaa gaaggaagaa gttaaaccag agaaagtaag
    20281 taaaaagaaa catctggagc aaactgattc agatatttct gaatttaaaa agggtaagaa
    20341 aaagcctaaa tatcatactt ttgaacatga tgaggaggaa caagaacttc atcgtcgtgg
    20401 tggtcgaagt aaatttaaaa agaaaaaagg aactgaaaaa tcggataaat acagagaagc
    20461 tgaagaaacg ttaacgcatg gttttgcgtt acctactgct ccaatagtca gagaagtatt
    20521 gatacccgag acaattacag ttgccgaact ggcaaaacgg atgtcagtaa aagctgctga
    20581 agtgattaaa gtcatgatgt ctttaggagc gatggcaaca attaatcagg tcattgatca
    20641 agaaacgtct gtaattgttg tagaggaaat gggacataaa ccggtaatta tcaaagagga
    20701 tgccgttgaa actggtttag gcgaagcaat ttccaaaggc acaaaaacag aaggaagagc
    20761 cccggttgtt acgattatgg gacatgttga ccatggtaaa acctctttac tggattacat
    20821 tcgtcgaacc aaggttgcag caggtgaagc agggggcatt acgcaacaca taggtgctta
    20881 ccatgtcagt acgccaaaag gtgacattac ttttttagat acgcctggac atgcggcatt
    20941 tactgcgatg agagctcgtg gtgcccaagc aactgatata gtcattctga tcgtggcagc
    21001 tgatgatggc gttaagcccc aaacaattga agcgatacag catgctaagg ctgctaaggt
    21061 tccaatcatt gttgcaatta ataaaatgga taaacccgac gctgatcctg agcgagttat
    21121 gaatgaacta tccgttcaag aagttattcc tgaggcatgg ggtggagata ccatgtttgt
    21181 taatatttcg gccaaatcgg gtatgggtat agatgatttg ctggatgcaa ttttattgca
    21241 atcagaagta ttagagttaa aagcagtgac tgatggtgct gcaaaaggag ttgtcattga
    21301 atcacgtctg gataaaggga gaggtcccgt tgcaacagtt ttagttcaaa gcggaacctt
    21361 gcataaaggc gatattttac ttgctggctt tcagtatggg cgtgtgcgtg ctttggttag
    21421 cgataatgga gatttggttg atagtgctgg cccatccatt ccagtcgaag tacttgggtt
    21481 atcggctatt cctcatgcag gagatgaagc ggtcgttgtc cccgatgaga aaaaggccag
    21541 agaagttgct ttgtttaggc agggcagatt ccgtgatgtg aaattggcca gacgtcaaaa
    21601 aaccacaata gaaggtatta tggaaaatat gacggctacc gaatccaaag tcttgaacat
    21661 tgtcttgaaa gcggacgttc aaggatcact ggaagcaatt tctgatgcat tgacaaaatt
    21721 atcaacagat gaagtcaagg tcgaggtgat atctagtggt gtaggtggaa taacagagtc
    21781 tgatgtccat ttggctatcg cttccaatgc cattttaatc ggatttaatg taagagcgga
    21841 tggcacagca aagcgtcttg ctgaacaaga atctgtatcg attcattatt atagtgttat
    21901 ttatgatata gtcgaccaga tcaagggtgc tttgacaggc atgcttgcgc cacaatttaa
    21961 agaagaaatt gttggtattg ctgaagttcg agacgtattc aagtcgccga aaattggtgc
    22021 tattgctgga tgcatggtca ttgaaggcgt tgtcaagcgt aataatccaa tacgtgtatt
    22081 gcgttcaaat gtagtgattt atgaaggtac tttagagtca ttaaggcgat ttaaagatga
    22141 cgtattagaa gtaagacaag gctttgaatg cggtatcggc gtaaaaaact acaacgatgt
    22201 gaagcctggg gatttaatcg aggtttttga gacagtcgaa attaagcgtg acttatagaa
    22261 aatgagtaat aattttaagc gaactgacag aattgctgag atgattcagc gcaaattagc
    22321 tttgatcatt ccccaggaaa taaaagatcc tcgcttgaag ggttttgtta ccatttctgc
    22381 agttaaagtg gctgcggatt taggtcacgc caaaatttat tttactgttc ttaatgaaga
    22441 taaatcggtg gtgaccaata tattaaatgg tgcagccagt tatttgagga gcgctctggc
    22501 aagaagtatt actctacgca ctgtgccgca acttcatttt atttatgatg aatcgattga
    22561 gtatggacag cggttaagcc gattaattga tgaagtaaat ccccctgatt caagtagtga
    22621 tgacaacaat tgaatcgaag tgttccatag atggaatcct gctattaaat aaaccacaag
    22681 gaatgacctc taacgccgct cttcagaaag ccaaacattt gtttggtgct aaaaaagctg
    22741 gtcacacagg cagcttggat cctttagcaa ccggcatgtt acctctatgc ttcggagaag
    22801 cgactaaaat atgtcagtat ctccttaatg cggataaaag ctatgaaaca attggaaggc
    22861 taggcagtaa gacaaatact gctgattgta ctggcgaagt gatattttgc attgaaaatt
    22921 atattgttag ccatgaggaa atgattgcaa ccctagaaaa atataagggt aaaaccaagc
    22981 aaattccatc catgttttct gctttaaagc acaagggaac ccctttgtat cgacttgcca
    23041 gagaaggtat agagatagaa cgtaaggcca gggatatagt aatcagccaa ttgaagttgg
    23101 aacaatttga tggtgaatgt tttacattaa cggtatcatg cagcaaagga acctatatac
    23161 gtaatttagt ggaagatatt ggtgatacgt tgaaggcagg agctcatatg acaaaattgc
    23221 ataggctcta cactgcaggg tttgaaaata atcaaatgta cactctcgat gaattacagg
    23281 atatgccttt atcacaaagg tttgcttgct taatccctat tgatcaggcc gttcaacatt
    23341 taacaccagt aattctttca gactctgagg taacagcaat tcgccagggc aaggtaattt
    23401 caaataaaac tagtgctgtt gaaggagaag atttgcgcct ttatggcgag caatcccaat
    23461 ttattggcat tggccaagct ttaattcatg gtgatattaa agcaaaaagg ttagtttctt
    23521 ttgcgttgta attacaattt ttgtaagaat tgccgcctac tttgtaataa gtatgaaatt
    23581 ttttacacaa atttatatcc actacttgtt tcatttgaca tgccttggta gaatgttcgc
    23641 ctttaaaaga atacttagct gagttccagg agtaaataat gtcgctaaat agcgcggaaa
    23701 aagcagaaat tattaatgaa tacaaacgcg gtgataaaga tactggttca cctgaagtgc
    23761 aagtttcttt aattaccagt cgtattaaat atttaactga tcattttaaa gaaaataaaa
    23821 aagacttcca ttcgagacgt ggcttgcaag agttggttaa caagcgccgc aagttactaa
    23881 aatatttgaa gcgtaacgat caagatcgct atcaaacatt aatccaaaat ctgggattga
    23941 gagattccta ttgattgata ggattattgt ctctatgggg acgcatcaaa taataaatca
    24001 gttttagaat aaaaggcgca gcgttctgcg cctttttttt cttatattta cttaatgggg
    24061 aacattacgt ggcaaaaatt acaaaagaaa tagtattcgg taaccataaa ttaatcttgg
    24121 aaacaggtga agtagcacga caggctgatg gtgccgtaat ggcaagtatg aatggtacac
    24181 aagtgctagt gactgtagtt tggaaaaagg atggtggtga aagtaatgat tttttcccat
    24241 taacagtgaa ttatcaagaa aagttctatg ccataggtaa aatccctggt ggctttaaca
    24301 aacgtgaagg acggccatct gacaatgaaa cattaatttc tcggttaata gatagaccga
    24361 tccggccatt attcccggat aattttttta acgaagtaca gataattgct actgtcctgt
    24421 ccttaaatcc cgaggtatct cctgatatta tcgctatgat tggagcctcc gcagcgcttt
    24481 caatttctgg cgtgccattt aatggcccta taggtgccgc tcgagtaggc tataaagatg
    24541 gcgtttatct gctcaatcca agtagaaaag aacaagaaga gtccaagctt gatttggtta
    24601 ttgcgggaac aaaagatgcc attttgatgg tggaatcaga agcgcaggaa ttaagtgaag
    24661 acatcatgcg tggtgctatg ttatatggcc atgaaatgat gaaaaatgtc ataaaatcaa
    24721 tagaagaact tgctagagac gtgggtaaga gcaaacctga gtggaaagca ccagaaattg
    24781 atacagtact aaaagctaga atcaatgacg tagctaggaa tgaagttgaa gccgcttatc
    24841 ttattaagga caagcaacaa cgctatcagc gattaggcga gttaagagag caaactattt
    24901 ctgctttact ggctgaaaat gatgagttaa atgcagatgt tattgccaat atgtttggtg
    24961 agctagaacg ttctatagta cgtaatcgta ttcttgacgg cgagcctcgc atcgatggtc
    25021 gtgatcacag aacagttagg cctatttctg ttcgtaccaa attcttggaa agaactcatg
    25081 gttcctgttt gtttaccaga ggagaaactc aagctattgt tgttgctact ttgggtaatg
    25141 aacgtgatgc acagatatta gacggaatta gtggcgaaag cagagatcga tttatgcttc
    25201 attataattt ccctccttat tctgtcggcg aaacagggca agtgggcagt cctaaacggc
    25261 gtgaaattgg gcatggccgt ttagctaagc gtgctttaat ggctgtactg cctgatgcta
    25321 atgagtttcc ttatgtacta cgtattgtat ctgagattac ggaatccaat ggctccagtt
    25381 ctatggctac tgtttgtgga acaagtctgg cattgatgga tgccggggta cctttaaaag
    25441 caccagtcgc tggtgtggcc atgggcttaa tcaaggaagg tgatcgttat gctgtattaa
    25501 cagacatatt gggtgatgag gatcatttag gtgatatgga ctttaaagtg gccggtacgg
    25561 aaaaagggat tactgctttg cagatggata tcaaaatttc cgggattacc aatgaaatta
    25621 tggaacgggc tctggagcaa gcattggaag gtcgtaccca tattcttggt gttatgaata
    25681 atgctcttgc cgaacataga accgagttat ctcagcacgc tccaagaatc acaaccatga
    25741 aagtcgctga agataaaatc cgcacaatta ttggcaaagg cggtgcaaca atcaaaggac
    25801 ttattgaaag taccggcgta tctatagata ttgatgactc aggggttatc caattatttt
    25861 ctccggataa gatggctttg gaagaggcgc aaaaacaaat taaagcttta attgctgaaa
    25921 ttgaagtagg tcagacttat caagggaaag taagcaagat agttgacttt ggagctttta
    25981 tcaatttact gcctggtaaa gatggcttac tgcatatttc acaaatatgt gccgacagaa
    26041 cacaaaaggt agaagaggta ttgcaggaag gtcaggaaat tgaagtcttt gttgctggga
    26101 tagataagca aggacgagtc aaactggagt ggaaggataa gccacaagct gaagccaaag
    26161 aggtcgaaga cgccccggta tctgctacgt ttcttacaat ggaagagcaa tctgaagaaa
    26221 ttaattcagg caacaaaatc tctgaagaag aggaataata agatagtagc atttcaagtt
    26281 ctatttatta taaactcgtg gaaggtatga gcttttcgcg agttttaact gagtgcttgc
    26341 aattggtata tatgaccgca taaattataa gctactaaag tagagtttaa gcatgaagct
    26401 attctctttt tttcttgtat tgataagctc tgctagttta ggtcagggtt taccattaca
    26461 tctcctaaag tacccgtccg gattttcagt aagtatttat gcagcccccg tttctgatgc
    26521 tcgagaaatg gcactgggca gtaaagacat agtttttgta ggttcaagaa gtgccgggaa
    26581 agtgtatgct gttattccag ataaaaaaaa ctcttatggt actcgagttt taaccatagc
    26641 ttctggattg aatatgccaa atggtgttgc ctttcataaa ggcgcacttt atgtcgccga
    26701 gattgggcgc attttacgct ttgataatat tgaatcaaat ctgaataatc ctcctgaacc
    26761 agttgtagtt actacaaaat taccgaagga aaaagctcat ggctggcgtt ttattagttt
    26821 tggaccagat gataagcttt atataggaat tggcgcccct tgcaatgttt gcttaatgga
    26881 agatccgcgc tatgccacta ttatgagaat gaatgccgat gggcaagatc tcgagatcta
    26941 tgctaaagga atacgtaata cagtggggtt tgattgggat ccctttcatc ataatctctg
    27001 gtttacagac aatgggcgtg actggctagg tgatgactta cctccagatg agctcaatgc
    27061 agctccgaga aaaggcatgg attttggttt tccttattgc tatggcaaaa gcacagcaga
    27121 tcctgtttat ggcaaaacac attcttgttc cgagtttact ccggctgttt ttgaattccc
    27181 agcacatgtt gctccacttg gtatgtcatt ttatacaggc aaattattcc cttccaatta
    27241 ttggggacaa atttttatta gtgagcacgg ttcctggaat cgtagtcata agatagggta
    27301 tcaaattact acagtaaaaa ttaagaataa tcacgtaact gaggttaaac catttatcag
    27361 cgggtggtta cagggggaaa aagtatgggg aagacctgtc gatactctcg ttatgccaga
    27421 tgggtcacta ttaatctctg acgattatgc taatgtaata tatcgggttg gctatcagca
    27481 gtaaatggag caattatttt attataggcg gggataaagc ccctacctat ttcttatcag
    27541 tattaacgaa ttaaatttaa gaattccaaa cggcaactcg aattgttacg catttcaccg
    27601 agcataactg aagttgtcat gacagaattt tgtttttcaa ctccacgcat catcatgcaa
    27661 agatgttttg cttcaatcac tacggcgaca ccgcgagctc cggtagtgga ttcaatacag
    27721 tgagcaattt cgcccgtcag ccgttcttga atttgtagtc tcctcgcata aaaatcaaca
    27781 atacgagcaa ttttagataa gcctattact ttaccatcag gtagatatcc aacatggcat
    27841 ttacccaaaa aaggcaataa gtgatgttcg cataatgagt acagttctat gtccttgacg
    27901 ataaccattt cactcatgtc tgaatcaaac agagcaccgt taatgatttc atctaggttc
    27961 tcattgtaac ctttagttaa atatcgtaag gcattagctg ctcgtccagg agtatccttt
    28021 aatccttccc gatttatatc ttcgcctaat tctttaagca aatttgagta taatttttcc
    28081 atagcaagaa ttcctaacct ggcggccaag tcatttgacg cccgccaagt aaatgtaaat
    28141 gaatgtggta aacttcctgc ccgccaccag aattaacatt aaaaactaat cgataaccca
    28201 tttcacttag tccttcggcc tgcgctaatt tttttgctct tattagaata ttagcaagaa
    28261 gctcgctatc atcatcgttc acatcattga tagttgcaat atgctttttg ggtataatca
    28321 ataaatgtgt tggtgcttga ggtctaatat cgcgaaatgc caaaatattt ttgtcttcaa
    28381 aaactactgt tgcaggaatt tcaccctgtg caattttgca aaacaaacaa ttcataggtc
    28441 atccgatgtt ttagattcaa aatgctatta tacatttcat ttattagatt gcgaacgctt
    28501 gtctttgcct tttgatgcga tttttcgcat aatattactt ttatttatta ttaagggtgc
    28561 gtaaatgagt ttaatggaaa ttccgagcgg tcgtgatgtt ccaaatgaag tgaatgtgat
    28621 catagaaatc cctatgcacg gagaacctgt aaagtatgaa gttgataaaa aaactggcgc
    28681 tttatttgta gatcgattta tgacgacagc aatgttttat ccaacgaatt acggttatat
    28741 cccgaatact ctttcagaag atggggatcc tgtggatgtg ttggtaataa ctcctgtccc
    28801 tttgatcagt ggggctgtta tttcatgtcg agctgttggc atgttaaaga tgacagatga
    28861 atccggagtt gatgcaaaga ttttggctgt tccaacaacc aaactaagta aaatgtatca
    28921 atccatgcaa acttatcaag atattcctca gcatttattg ttatctatcg aacatttttt
    28981 taagcactat aaagacctgg aagaaggtaa atgggttaag gtcgaaggct gggtaggccc
    29041 agatgcagct cgtgaggaaa taacatccag tattaaccgt tataatcaca taaaaaagta
    29101 aaacagtgac ttgaactgtg ccaggtgttg ggcattgctc agcaccaccc ttatatcttt
    29161 agctataata gcggcatcat ttcagcccct actacaaaac agcaagttct attgtataat
    29221 ttgtcattct taaaagtata aggatattca gtgcaacttc ctctttctac tttgttggtt
    29281 ttattgtttt tcctaatttg cctgtctgct tttttttcag gttctgaaat tggcatgatg
    29341 tctatcaata gatataaatt acgacacctg gtaaaaagca atgataagca agccatgcgg
    29401 gttaactcca tgctggctcg gcctgataaa ctactaagtg tggttttaat tgggaatact
    29461 ttggcaaata tagtggcatc gactcttgcc actttaatcg ggcagcggtt ttatggggat
    29521 gcaggggtgg ctatagcaac tgctttgttg actatactga tcctggtttt tgcagaaatg
    29581 actccgaaaa ctctggctgc tatttaccct cagcaagtcg cttttgcatc ctcattgccg
    29641 ttgagcattt tacaatggat tttttctcct ttggtccact taatcagttt gattaccaat
    29701 ggtatcctaa gagtatttaa catatcggta aaacgtatgc aaaaggagat gctaactggt
    29761 gaagagttgc gatcagttgt gcatgaggcg ggaggattaa tgcctgtaga gcataaaagt
    29821 atgttgatta gtttattgga tctggaacaa gcaactgttg aagacatcat ggttcccaaa
    29881 gccgatattg ttgggattga tttggattta ccctggatag aacttttaga gcaactggaa
    29941 acagcacaac atacaagatt accaatttat catggttcta ttgatcattt ggcagggatg
    30001 atccacgtaa gaaatatttt aaatttagca ttagaaaata agttggatat ggagaatttg
    30061 ttaaatagtg cagaagttcc ctattttatt ccagaggcta ccccattaaa tatacaaatc
    30121 cttaatttca ggaaaatgaa aagaagaagt tgctttgttg tggatgaata tggcgatatt
    30181 cttgggcttg tgactatgga agatattctt gaagaaatag taggtgaatt cacaacagat
    30241 atagctgctt taagccgtga tataactcct cagcaagatg ggtcggtcat agttgatgcc
    30301 agcataacta ttcgccattt aaatcgcatg atgggctgga gcctgccctt aatcggtcca
    30361 agaaccctaa gtggcctgat tatcgagcat ttagggtata ttcccccccc tgactcttgc
    30421 ctggttattg atcattatca tattgaaata ttaagggtca gcgataatat gataagaggt
    30481 gtaaagatga ttaagactaa taaaaaaaga aaatagctcg tatagactct gactctcaat
    30541 ttgcttccag cttgtcccta ttt
//
LOCUS       DBNCWK010000023        29757 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.023, whole genome shotgun sequence.
ACCESSION   DBNCWK010000023 DBNCWK010000000
VERSION     DBNCWK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29757
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.023"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(69..1859)
                     /gene="oadA"
                     /locus_tag="AC06IO_000956"
     CDS             complement(69..1859)
                     /gene="oadA"
                     /locus_tag="AC06IO_000956"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HIO9874164.1"
                     /translation="MSKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAME
                     VWGGATFDACLRFLKEDPWSRLRQLRQALPNTQLSMLLRGQNLLGYRNYADDVVRAFV
                     KLAVNNGVDVFRVFDALNDARNLKVAIDAIKSQKKHAQGAICYTTSPVHTLDNFLELG
                     EKLAEMGCDSIAIKDMAGLLTPAVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEA
                     VLSGCNHIDTAISSFSGGASHPPTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKK
                     YSQFESEAQNIDPRVQLYQVPGGMISNLYNQLREQNALDKMDAVHKEIPRVRKDLGYP
                     PLVTPTSQVVGTQAVINVLTGERYKTITNEVKLYCQGKYGTPPGKISSALRKKAIGRT
                     EVIEVRPGDLLPNELDQLQNEISDLALSDEDVLLYAMFPEIGRQFLEQRKNNQLIPEP
                     LLTQSSAPDNSVMSEFDIILHGESYHVKVAGYGMIEHGQQSCFLWVDGVPEEVVVQHS
                     ELHDKIERSSVNNKIGPGDITVAIPGSIIAIHVSSGDEVKAGQAVLVIEAMKMETEIK
                     APANGVVAEILCQKGDKVTPGQVLIRVEVS"
     gene            2061..2498
                     /gene="aroQ"
                     /locus_tag="AC06IO_000957"
     CDS             2061..2498
                     /gene="aroQ"
                     /locus_tag="AC06IO_000957"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIO9874165.1"
                     /translation="MKKILVLHGPNLNLLGSREPSIYGHDSLTQINGDLIQEADNAGI
                     RLSCFQSNAEAELIQAVHQAGIDKINYIIINPAAFTHTSIALRDALSAVAIPFIEVHL
                     SNIFSRETFRHHSYFSDIAVGVISGLGTKGYLLALQAIIKELK"
     gene            2512..2994
                     /gene="accB"
                     /locus_tag="AC06IO_000958"
     CDS             2512..2994
                     /gene="accB"
                     /locus_tag="AC06IO_000958"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIO9874166.1"
                     /translation="MDIRKIRKLIELLEETGISEIEIKEGEESLRLSRHSNIVAETAP
                     QIHYVAPSAVKQPPPTPSSNTSTTEPHVIENKQVTSASSGHKIRSPMVGTMYTAPSPE
                     SPPFVTVGQSVKAGDTLCIIEAMKMFNEIEADRAGKIVEILVKNGDPVEYDQALFIIE
                     "
     gene            3007..4350
                     /gene="accC"
                     /locus_tag="AC06IO_000959"
     CDS             3007..4350
                     /gene="accC"
                     /locus_tag="AC06IO_000959"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIO9874167.1"
                     /translation="MLSKIVIANRGEIALRILRACKELGIQTVAVHSDVDKDLLHVRL
                     ADETVCIGPAPAQKSYLNIPAIISAAEITDAVAIHPGYGFLSENADFADIVEQSGFRF
                     IGPRGDSIRLMGDKVSAIAAMKAAGVPCVPGSDGPLGDDDNKNLELARKIGYPIIIKA
                     AGGGGGRGMRVVHSESNLLSAIALTRSEAKAAFNNPIVYMEKFLENPRHVEFQVLGDG
                     KGHAIHLGERDCSMQRRHQKVVEEAPAPGITPELREEIGASVIKACKELQYRGAGTFE
                     FLFQDGCFYFIEMNTRIQVEHPVTEMITGIDLIKEQIKIASDIPLTLTQKDIKLRGHA
                     IECRINAEDPRNFMPSPGTIKLLHQPGGPGIRFDSHIYSSYVVPPNYDSMIGKLISYG
                     ATRAEAIARMRNALDEIIIDGIKTNIELHQRILHDKSFMEGGTNIHYLEKMLKDS"
     gene            4352..5221
                     /gene="prmA"
                     /locus_tag="AC06IO_000960"
     CDS             4352..5221
                     /gene="prmA"
                     /locus_tag="AC06IO_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIO9874168.1"
                     /translation="MWFQLKIEHCPNDKIEEITEELEECGALSITLTDKNDNPVLEPE
                     PGTTPLWPEVIIHALFAQAEEAQYAREQLVAKRPSLHCSLELLADKNWERAWMDDFRP
                     QRFGNRLWVCPTWLPPPEPDAVNLILDPGLAFGTGTHATTSLCLTWLEQADLKNKSII
                     DYGCGSGILSLAAIKLGAKHVYAVDIDNQALQATQSNAHANHITESQLSISSPEALQN
                     PVHLVIANILLAPLISLKERFHQLLPSGAHLVTSGILEEQAPLLIDAYDSAFTHIATE
                     YCEGWSLLVFTSK"
     gene            5248..6837
                     /gene="purH"
                     /locus_tag="AC06IO_000961"
     CDS             5248..6837
                     /gene="purH"
                     /locus_tag="AC06IO_000961"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIO9874169.1"
                     /translation="MAKKQIYSSFKPRRALLSVSDKRGIRELGQALHDQGVELIATGN
                     TAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAIHAGLLARGEQDSPVLRQHGIKP
                     IDLLIVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSK
                     LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFN
                     KVTDLRYGENPHQQAIFYADKNSHPGSLSTATLLQGKQLSYNNILDADAALDCVKSFS
                     NEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAIL
                     EKQFVEVIIAPDANEEAKKILAAKENIRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHD
                     SLSLESCELQTVTQIKPTDKQLQNLMFAWLAAKHVKSNAIVYANDLATIGIGGGQTSR
                     VMSARIGLWQAEQMGFDPKGAVMASDAFIPFPDTIEIAAKAGISAIIQPGGSIRDEKI
                     ISCADQHNIAMIFTGVRHFKH"
     gene            7122..7907
                     /gene="icmH"
                     /locus_tag="AC06IO_000962"
     CDS             7122..7907
                     /gene="icmH"
                     /locus_tag="AC06IO_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HIO9874170.1"
                     /translation="MTTEQYPSSLVNRLAITEPALVPSGYYRSKLFIAPFSTNVLVAA
                     AGPILSLLERLCLSPSLPPVEDIRENIQHELRAFHSKLDASKYPLDIISIAQYLLSAT
                     IDEILGKSYLRVYNLTTEFKSFTPLSSDGAQPQQRFFEILNYIKERPNQFLDLIELAY
                     FCLIAGFEGEYHLKADGRQCLDNTIEDLYQIIQKYRFNKPHRLFNENPLPKTIKKNYK
                     ATWITTIAAVGIVILAFVTSHVLLENKAKTVLFGHTQLAMLEN"
     gene            7907..10828
                     /gene="icmF"
                     /locus_tag="AC06IO_000963"
     CDS             7907..10828
                     /gene="icmF"
                     /locus_tag="AC06IO_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmF"
                     /protein_id="HIO9874171.1"
                     /translation="MDNSLRALCDAIKKILSQLKPQANQLSFIVITGKNAQGKSALLK
                     QSNMEEMPVFSEEHAKLYFNQKGIIVELGENWLTNSKTLLLTTLKQLNKCNRHLKITG
                     LILCVDVNDLLIAEPAQFTEQKKSHLQLIQRLGVNLGYQAELALIFTKMDTLAGFSEF
                     YQMDHVNDLSKPLGFSLDCVNQLSKKIETFSLQFNQLIEALGQQVINKMHPARSTIKR
                     SLIREFPLQLASLRAPIQSLIQGISPKLFHVHSIFFTSAEQGGVSVDRLNKKIQHEYA
                     LVVQDTFPQATNFRSYFVEGALQTIQEQCIQVPQTTKFSQKPFIAVAVSLAGLSLLTL
                     IYNHYKTSHILDEASKELLAYEALNNQGTSGAQALYHLSQAANKMNHITSNSISLPTV
                     HQLKLNLYNNAQNRLQGEFLPSLANELEQVITSPGNTPITRYKALKIYLMLGDSKYFS
                     YQPILDWFQQQWQGRAPGTTQKQLALLKQTLSNPPQNIPIKSQIISDARNYLNALPTS
                     YLYYSIAKEYFPQEKQKIVIEGFSIPTDEVPTYFTKTGFTKIINELPKITSTLQSENW
                     VLARQDLSQLHNMLIQAYCFDYVTWWQTFMKKSQPLRYQDYQQGRQLTKLIQQSNSFY
                     KFANLIQQETKPDLTEGISTFNQFIANQFTDLNLTTLSSIKELDFRISELERFIATLS
                     VVNDGGKTAFTITRSRFLNENSSDPVSLLYSQARQLPEPLSSWTKQIAGDTWVILIRD
                     SRQYINNQWQKTVYREFQNSIAQRYPFDSSQTAEIDIADFNRFFSTHGLLNTYSEQYI
                     KPFLDTSSPEWKPKAVNDFILPISNEVIDELIRANIITNMFFPDHSDESKIDFSLQKI
                     SLDPVVSNLVLEIGGTKLMDNQTSDSLIRFTWPQNNAKLALDSIEGNHYELAEYGTWA
                     IFKLLEKVNVLVDEQDSSSLQILFEVNSNSGRYLLKTNNQINPFTPGILNGFTLNDHI
                     A"
     gene            10831..12087
                     /locus_tag="AC06IO_000964"
     CDS             10831..12087
                     /locus_tag="AC06IO_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874172.1"
                     /translation="MKQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHF
                     VSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKALILSMSVMTISTALIAVLPDFN
                     SWGIIAPLLFSVLRLIQGLAVGGELPGSTTYLIEHMFQQSRGLAGSLVLSSAFLGIFI
                     GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAEL
                     PAKVVFTRFKQQLFLSVIITSILAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALA
                     LLTVLIPLMGFLSDVVGRKPIFLIGLFALCLLIFPFFWLLLSGSWWQALCSELLLAIA
                     LAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFTLIELSSNKMAPA
                     WYLFVWTLIVIPITISIRESYQKELT"
     gene            complement(12275..15304)
                     /gene="icmB"
                     /locus_tag="AC06IO_000965"
                     /gene_synonym="dotO"
     CDS             complement(12275..15304)
                     /gene="icmB"
                     /locus_tag="AC06IO_000965"
                     /gene_synonym="dotO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmB/DotO"
                     /protein_id="HIO9874173.1"
                     /translation="MANWSESFFEGVDTFFAWLSTSLKQTTESYIDLETADSPTVLVN
                     HDGSLLSVLKIEGITGLAGPEEFERLVEGLANSFQAAMGRPGHALQVYFSHDKQNIVK
                     MIKDIYDPAEATASRLELNLRDLFEERVNYLSQYCAEERVYFVLYTRPFNLASDQLKA
                     ANKAKMKMIKDTKAPPFKNTQTIFAAVPEIRDTHDAYVRAILNDLDALNVIAKLLEVH
                     DAVHAIRMTGDPDYTADDWRATLPGDKIPVRELNNFEGDPSDLLWPPLSRQVLPRDAE
                     IIDLRTVRVGDKIYASTYIDLFPKDVRPFISLFSRILPSHVPWKISFLLESEGLSTIK
                     LKGLLAAILSFSSAQNRLISDSVNLLKYIQLNTDEAIVRLRVVATTWAPEGNIPLLRR
                     RSSELVKAIEGWGSTDVSEICGDPFAGFVSSMLATTLNSSAVASVAPLSDVISMLPIT
                     RPASPWKTGALLFRTPDGKPWPFQPGSTEQTTWIDLVYARPGSGKSVLSNALNLALCL
                     SGGLMRLPRIAIIDIGPSSSGLISLLKEALPASKRHLVAYHRLRMTPDYSINPFDTQL
                     GCRYPTALERSFLVNFMTLLTTPLGAAKPYDGMADLAGMVVDELYKSMADEFNPTPYA
                     PGIEEFIDSILEEIGFVRDAKSTWWEVTDALYSAGFVHEAMLAQRYAMPLLADAASIC
                     RTPSIEDLYEKITAPTGESLINAFSRMISSAVREYPILSRVTSFDIGDARVVSLDLDE
                     VAKSGGDAADRQTAVMYMLARYVLARHYYLTEESLNNVPEQYKEYHKQRVLEIREDHK
                     RIVYDEFHRTAKSSAVREQVIIDMREGRKWKVQISLLSQAVDDFDPVMIDFATAIYVM
                     DAGPSQAVEKTSQIFGLSETAKIALRTRVHGPRQGGATFLAQFATKTGINVQLLTLTL
                     GPVELWAFSTTAEDATVRNLLYRHLGPSEARRLLAALFPNGTVTKELEARLASMKQKV
                     GLIEDDEREGMINQLVNDILDAYSKDPNVKSLPAKVG"
     gene            complement(15340..15966)
                     /gene="icmJ"
                     /locus_tag="AC06IO_000966"
     CDS             complement(15340..15966)
                     /gene="icmJ"
                     /locus_tag="AC06IO_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmJDotN"
                     /protein_id="HIO9874174.1"
                     /translation="MADNQQRCELKLIASPGSWRLYSARKIDERFKSYEQKIFQRDRY
                     TCQFCGFQARLYQDIVNLDGDYTNNRLSNLVTACCFCAQCFFVESVGVGGYGGGTLIY
                     LPELTQAELNSLCHVLFCAITNDTGYKSSAQNIYRSFKFRSQIVEEKFGEGTSDPAIF
                     GQLMIDSGVNSEEIREKLFKNIRLLPSRAKFRKQIEKWAASALEEIAD"
     gene            complement(16164..16562)
                     /gene="icmD"
                     /locus_tag="AC06IO_000967"
                     /gene_synonym="dotP"
     CDS             complement(16164..16562)
                     /gene="icmD"
                     /locus_tag="AC06IO_000967"
                     /gene_synonym="dotP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmD/DotP"
                     /protein_id="HIO9874175.1"
                     /translation="MNIKVIHKSGYKHWLVSAACLALLTLICQDAAAGVSLGKMASQI
                     TGSFTSLTKLITAGSYLAGLGFSIGAIMKFKQHKDNPTQIPIGTPIALIFIAAALLFL
                     PSILGVAGVTMFGGSGGKTAGPTGTIYKTN"
     gene            complement(16588..17172)
                     /gene="icmC"
                     /locus_tag="AC06IO_000968"
                     /gene_synonym="dotE"
     CDS             complement(16588..17172)
                     /gene="icmC"
                     /locus_tag="AC06IO_000968"
                     /gene_synonym="dotE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmC/DotE"
                     /protein_id="HIO9874176.1"
                     /translation="MADPTCTGGTVACWIVSQANILANIANQLEPVQRLITGGAYLIG
                     CAFIFKAIYSLKVYGEARTMMSSNTSIKEPVMYLMVGALLIYFPSLVSSVLQTTFGYS
                     NPLAYSGGVSSGSDTISALFGSGSLVGRPLVMIIRVIGLIAFVRGWVLIARSASQGQP
                     PGGTGKGLIHVFGGILAINIVGTVDMINNTLYGT"
     gene            complement(17180..17989)
                     /gene="icmG"
                     /locus_tag="AC06IO_000969"
     CDS             complement(17180..17989)
                     /gene="icmG"
                     /locus_tag="AC06IO_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmG/DotF"
                     /protein_id="HIO9874177.1"
                     /translation="MMAEHDQNNDEYKFAELDSYDMDQAGESDLDSEASFQSGKEGLT
                     KKKDIKRNALIAIGAVVLIMVMYKIIGWMFFSDKSSQVTSKPAIPPVTQVATPQPVQT
                     IPTTTPIQQVQPTTIIEDDSDLKKKVSAIEMTQQSLRSEVNALGEQINAVNNNIKNLN
                     AQIVNLNQIIGNMSNQIARQSEVINVLMARTTPKKVVKVSRPTVQARIIYYIQAVIPG
                     RAWLIGSNGSTLTVREGSKIPGYGMVKLIDSLQGRILTSSGQVIKFSQEDS"
     gene            complement(18004..21150)
                     /gene="dotG"
                     /locus_tag="AC06IO_000970"
     CDS             complement(18004..21150)
                     /gene="dotG"
                     /locus_tag="AC06IO_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotG/IcmE"
                     /protein_id="HIO9874178.1"
                     /translation="MASKKENLKSLFSNTRTRVIIVFTAALLIIAVVIGFFKIRSATT
                     SSIAAAEVSTVPGGIQSIPGVLDPTAQYAKLQEEQNITQAQVAEKTGGSAIPTIIRTQ
                     ALGEGVGVIGSQSGVGFAALAQEELGGPQRSLWIQELQDGGCSKSVITKVMDQGAQLT
                     DLKAACSCVQLKDSGYGLQELEQVCECKELKAAGYNARQLKEAGYSAGRLRNCGFDAC
                     ELRNAGFTAQEMKDGGFSDGELKGAGFSDAEIAKASGLPDGITADDVRKAGCGAAALA
                     KLRQAGVSASAIRKISGCTAEQLKAAGYTAKELKDAGFSAADLKRAGFSAAELKDAGF
                     TARDLLNAGFTPADLAKAGFSNAQIKAAQAELPPGITPQDVKNAGCDVEALKKEREAG
                     VSAALIRQYAGCSAQALKAAGFTDADLASAGFTPAQISAATPLSDAEIKAAGCDPDKL
                     KKLFSAGVSAKRIKELNGCSAEALKAAGYDAQSLLAAGFTPQELLAAGFTPKQLEDAG
                     LNPASIIADGRVADCSVESLKKARAAGVSALTIKQTLGCSAAALKAAGYTAKELKDAG
                     FTAAELKAAGFSAKDLKDAGFTAKELRDAGFSAQELKDVGFSAKDLKDAGFSAAELKA
                     AGFTAAQLKAAGFSAKDLKDAGFSAAELKAAGFSAKELKDAGFSASDLKNAGFSAKEL
                     KDAGYSASDLKSAGFSASELKNAGYSADELKKAGYTSAELRNAGFSPQESAVAGLQGP
                     DLQQLDSSITGIPSIPGATPRPTTSDAASSAEQLQAILQKQNEQLAEQKYQQEIQQRT
                     SDMLTAATQLVQDWKKVETQVYTEGTEETKTSGGESSVPGAGTGAGANNQPVEQGVSG
                     AQNQAIIKTGDIMFAVLDTAVNSDEPGPILATIVTGKLKGSKLIGSFNLPSNADKMVI
                     TFNTMSIPGAEKTISISAYAIDPNTARTALSSRTNHHYLMRYGSLFASSFLQGFGNAF
                     QSANTTITIGGTGGGNNITVANGVGRSTLENAVIGLATVGKAWSQQAQQLFNTPTTVE
                     VYSGTGLGILFTQDVTTI"
     gene            complement(21155..22237)
                     /gene="icmK"
                     /locus_tag="AC06IO_000971"
                     /gene_synonym="dotH"
     CDS             complement(21155..22237)
                     /gene="icmK"
                     /locus_tag="AC06IO_000971"
                     /gene_synonym="dotH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmK/DotH"
                     /protein_id="HIO9874179.1"
                     /translation="MKKYDQLCKYCLVIGLTFSMSCSTYAADQSDDAQQALQQLRMLQ
                     QKLSQNPSPDVQSGAGDGGDNAASDSTQQPNQSGQANAPAANQTANAGGDGQIISQDD
                     AEVIDKKAFKDMTRNLYPLNPEQVVKLKQIYETSEYAKASTAGTPPKPTATSQFVNLS
                     PGSTPPVIRLSQGFVSSLVFLDSTGAPWPIAAYDLGDPSSFNIQWDKTSNTLMIQATK
                     LYNYGNLAVRLRGLNTPVMLTLIPGQKAVDYRVDLRVQGYGPNAKSMPTEEGIPPSAN
                     DLLLHVLEGVPPPGSRRLVVSGGDARAWLSNEKMYVRTNLTILSPGWLASMTSADGTH
                     AYEMQKSPVLLVSWHGKVMQLKVEGL"
     gene            complement(22240..22878)
                     /locus_tag="AC06IO_000972"
     CDS             complement(22240..22878)
                     /locus_tag="AC06IO_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system apparatus protein
                     IcmL/DotI"
                     /protein_id="HIO9874180.1"
                     /translation="MAEDALTVVALRNKFYKDSQRKVILALLIAIVVNVVLASLVVYM
                     ITHPPAPKYFATSINGRITPLFPLDEPNQSDSAVLQWANQAAIAAFTYNFVNYRDELQ
                     ASSGFFTAEGWDQFLGALEQSNNLDAVKAKKLVVSAVATRAPIILQKGVLNGRYSWRV
                     QMPILVTYQSASEFTQQNNVVTMLITRVSTLNSPRGIGISQFVVGPASGGVS"
     gene            complement(22894..23178)
                     /gene="icmM"
                     /locus_tag="AC06IO_000973"
     CDS             complement(22894..23178)
                     /gene="icmM"
                     /locus_tag="AC06IO_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmM/DotJ"
                     /protein_id="HIO9874181.1"
                     /translation="MSRETWALIKRNKNFNVHIYRRGLTLLILSLGLSCGLSGLIYYV
                     HIHEPERDFYATSGITPPIQLKPMLTPNMSSEALLPPDPPDEDSRRIIPQ"
     gene            complement(23190..23759)
                     /gene="icmN"
                     /locus_tag="AC06IO_000974"
     CDS             complement(23190..23759)
                     /gene="icmN"
                     /locus_tag="AC06IO_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmN/DotK"
                     /protein_id="HIO9874182.1"
                     /translation="MRSLRTNYIYVLFKTTGLLFLLLLSACNRSGYIPENEVPKLPCR
                     VDGACDATIIKMMTDLNKKGIKVASVGQNYLISIPASALFADQSPRLNWASYSLLNEI
                     AAFLKQFRKIAITVTSYSSKYVSVKRERALTLARSRVVSEYLWSQGVDSRIIFTQGLG
                     SDKPITSYTLGGDRSPNARVEITFRRAVA"
     gene            complement(24163..26514)
                     /gene="icmO"
                     /locus_tag="AC06IO_000975"
                     /gene_synonym="dotL"
     CDS             complement(24163..26514)
                     /gene="icmO"
                     /locus_tag="AC06IO_000975"
                     /gene_synonym="dotL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmO/DotL"
                     /protein_id="HIO9874183.1"
                     /translation="MMRGIDSRHELDPTLLLRDTRTFTQRLADFFADPTNISIVLISL
                     AAVSYYFSEAATFLLIMGGIFFLYSYTRKQKLPFRLPQISRAKDYNDLKPGINKPNIA
                     RGITFFGNDRKTGEELWFANDDMRTHALIFGSTGSGKTETLVSLSYNALVQGSGFIYV
                     DGKGDNSLYAKVFSMVRSMGREDDLLLINFMTGARDIVGPQEKRLSNTLNPFCQGSSS
                     MLTQLVVSLMGSSGQSSDGDMWKGRAIAFVEALMRLLVYMRDEGAILLDANTIRNYFD
                     LQRLESIVIDKLFPRDDQESVNIETIPKLVTDPLRNYLNTLPGYNKEKKGKQVSQVLE
                     QHGFITMQLVRSFSSLADTYGHIIRTNLAEVDFKDVVLNRRILVVLLPALEKSPDELS
                     NLGKIIVSSLKAMMAAGLGEEVEGDYRDVILRKPTNAPTPYMCILDEYGYYAVQGFAV
                     VPAQARSLGFSAIFAGQDLPAFQKASKEEAASIGANTNIKICMKLEDPTETWDFFTKT
                     AGEAYVTKVDSFQTKETSIANSYMDTKSSSFEKRARVDLLDLKEQTEGEAHIFFKSKI
                     VRARMFYANPKPVKQLKINQFLKVEPPPDDYLMKLQKQLASFQSILESGDLSINKAVE
                     NEEITLISKALKESTIVEPIERGVAALIAFHGQNEPEPVEDIVEEEVEGALTIFSKLR
                     IDPNAPPILVSDKEVFSEPLLPINETRNQMITIERLAGAKDKYAGTVANELIKDFQIA
                     TSYPPEERDVIDVQELTGVIRDLSAKISAEREKANKKAAEELT"
     gene            complement(26511..27641)
                     /gene="icmP"
                     /locus_tag="AC06IO_000976"
     CDS             complement(26511..27641)
                     /gene="icmP"
                     /locus_tag="AC06IO_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system coupling complex
                     protein DotM/IcmP"
                     /protein_id="HIO9874184.1"
                     /translation="MAQQQQQSGSDNSMAPVWIVILLFITAYFVWALAHQYIVSFVFT
                     INIWQARLVNLFLNNQLLANQIYLMQTLDPNTVNWDQMVTVMRAVGDYMRYPVICILV
                     VLAFVLYNSNVTLKYRKTYDMKSLRAQEQFNWPAIMPIVKEDLVSQDVNKGPWAMALT
                     PMEFARKYNLLRKDDALLDNPVPGEEMTAGIRRGDAKRVFTMQLGPYWDGFERCSPQA
                     YALSAVFMARMNRDRDAANNILKVLDKTFVDGKPDFSVARPVMKKYQNSELVQEVVAK
                     HAYVLTVIASLLEAAREDGVVPSSEFLWLKPVDRRLWYMLNCVGRQTPYSEVAGPFAH
                     WKAEKEMGRRSLVPMIDEAIRALEIAVKEVRLTPRQMEELEP"
     gene            complement(27828..28403)
                     /gene="icmQ"
                     /locus_tag="AC06IO_000977"
     CDS             complement(27828..28403)
                     /gene="icmQ"
                     /locus_tag="AC06IO_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm secretion system protein IcmQ"
                     /protein_id="HIO9874185.1"
                     /translation="MKDQLSDEQKETILKALNDAIEKGPWDKSNFLRVIGKKLIAIRD
                     RFLKRIGAASQAKLKAESHLANRIALRSGQQEIYVSLYSSDGSNLQSWEKIVGSLPRQ
                     MISRPIYADEEDIKAILKTKENKQNEAYVAIYISQSDILHLSADKAPVDKLGKPLLTL
                     KDKSISLENISRFVHVSGVYRYSNGRLIKNA"
     gene            complement(28494..28856)
                     /gene="icmR"
                     /locus_tag="AC06IO_000978"
     CDS             complement(28494..28856)
                     /gene="icmR"
                     /locus_tag="AC06IO_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmR"
                     /protein_id="HIO9874186.1"
                     /translation="MGNNTDDSARNPFGFYTPPRVKEIGEPDVTDATLGSVYSEIISP
                     VKDCILTVAKAVSFNPGGKDNTDAVEVLTELNTKVERAALNQPILTTKTERMFGAAES
                     EKSSEQPSHEERGFKLSS"
     gene            complement(28956..29300)
                     /gene="icmS"
                     /locus_tag="AC06IO_000979"
     CDS             complement(28956..29300)
                     /gene="icmS"
                     /locus_tag="AC06IO_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmS"
                     /protein_id="HIO9874187.1"
                     /translation="MERDISKCMAKIAASMNAKFYLNDRFVSFDEVFSETGLLPAIAK
                     RADQLCSLCLGYGLGATYDESEGALLGIRVVFDEVTPNVLRLLCMTDVMNELIQGGPS
                     RDYTPLDELMYD"
     gene            complement(29301..29561)
                     /gene="icmT"
                     /locus_tag="AC06IO_000980"
     CDS             complement(29301..29561)
                     /gene="icmT"
                     /locus_tag="AC06IO_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmT"
                     /protein_id="HIO9874188.1"
                     /translation="MAGGFSATSHWRDSARSARFFVVDARAAFPIFLFLMHIRVWTGV
                     LVLVSAIFFGIIEHYGFTVPVFLRWLRSFLAGSIRSARPWWR"
BASE COUNT         9022 a         6485 c         5299 g         8951 t
ORIGIN      
        1 ataaaaattt ttacataatt ttggaatagg caaaaacaag tagtgaaaat aatacactta
       61 actacccgtt aactcacctc cactctgatt aatacttgcc ctggagtcac tttgtctccc
      121 ttttgacata atatttctgc aaccacccca tttgccggtg cttttatttc tgtctccatt
      181 ttcattgctt caataaccag tacagcctgc ccggccttga cttcatcacc agaagaaaca
      241 tgaattgcaa taatagaacc ggggatagct acagtaatat ctcctggtcc tatcttgtta
      301 ttaacagatg aacgctctat tttgtcatga agttcagaat gttgcacaac aacttcttca
      361 ggaacaccat caacccacaa aaaacaggat tgttggccat gctcaatcat tccataacca
      421 gctactttta catgataact ctcaccatga agaataatat caaactcaga catgacggaa
      481 ttatctggtg cacttgattg agtcaataat ggctcaggaa ttaattgatt atttttacgc
      541 tgctctaaga attggcgccc aatttcggga aacattgcat aaagaagaac atcctcgtca
      601 cttagtgcca aatcacttat ttcattttgc aattgatcta attcattagg taacaaatcg
      661 ccaggcctga cttcaatcac ttctgttcgt ccaattgctt ttttccgtaa agcagagcta
      721 attttgccag gaggagtgcc atattttccc tgacagtaga gtttcacttc attggttatt
      781 gttttatacc gttctcccgt tagaacatta ataactgcct gagttccaac gacttgagat
      841 gttggcgtaa ccaaaggcgg ataacccaag tccttacgaa cgcgggggat ttccttgtga
      901 acagcatcca tcttgtctaa tgcattctgc tcccttaact gattatacag atttgagatc
      961 atgccacctg gcacttgata caattgaaca cgagggtcaa tattctgtgc ctcactttca
     1021 aattgagaat acttcttgcg gacggcttta aaataatcgt ctatttccaa taaaatattt
     1081 aaatccaatt ccgtgtcata cggagtatca gtcaatgcgg caaccaatgc ttccgtaggt
     1141 ggatgagatg caccaccaga aaaagatgaa atcgctgtat caatgtgatt acaacccgat
     1201 aaaacggcct cataatggca tatacttgcc aaaccggaag tagaatgact atgcaaatgt
     1261 acaggtaagc cagtcgcttg ttttaaaccg gcatacaatt cgacagtcac cgctggggtt
     1321 agcaaacctg ccatatcttt tatggcgata ctatcacatc ccatttcagc caatttttcc
     1381 cctagctcca aaaaattgtc caaagtatga accgggctgg ttgtgtaaca aattgctccc
     1441 tgggcatgtt ttttctgtga tttaatcgcg tctatggcta ctttaaggtt tcgcgcgtca
     1501 tttaaagcat caaacacacg aaatacatcc actccattat tcacggccaa tttaacaaaa
     1561 gctcggacta catcatctgc ataattccta tagcctaaca aattttgacc tcttaatagc
     1621 atagagagtt gagtattcgg taacgcttgg cgaagttgac gcaatcttga ccagggatct
     1681 tcctttaaaa atcgcaaaca ggcatcaaaa gtagcgcctc cccagacttc catagcccaa
     1741 aatcctacat catccatctt attacagatt ggcaacatat cttcagtacg cattcttgta
     1801 gcgattaagc attgatgggc atcacgcaag gtgacatctg ttataaacgt tttagacata
     1861 agctcttcct ggctcaagaa ataaataaaa atatcataat cctaaaattg gtacactctt
     1921 taagtacaat ccattgcttt ttactaaata taaaatttct tgctaaactt gcggagatct
     1981 tgccgatatc ctcgcagata tatagacatg ttcataaagt tcaggcaaaa tctgcaaatc
     2041 tcagactata ctgatgtgta atgaaaaaaa ttctagtatt acatggtcct aatctaaacc
     2101 ttttaggctc ccgagaaccg tccatttatg ggcatgattc gttaactcaa atcaatggtg
     2161 atttaatcca ggaagcagac aatgcgggga ttcgactcag ctgctttcaa agcaatgctg
     2221 aagcagagtt aattcaagct gtccatcagg ctggaattga taaaatcaat tacattatca
     2281 taaatcctgc agcatttact cacacaagca ttgctcttag agatgcttta agtgcggttg
     2341 ccatcccatt tattgaggtt catttaagta acattttttc ccgtgaaact tttcgtcacc
     2401 attcctattt ctcagatatt gccgttggtg tcattagcgg tttggggacg aaagggtatt
     2461 tactggcctt acaagcgatt atcaaagaat taaagtagag agaaaaaaca aatggatatc
     2521 agaaaaatca gaaaacttat tgaactcctg gaagaaactg gtatttcaga gattgaaatc
     2581 aaagaaggag aagagtcgtt aagactaagt cggcatagta atatagtagc agaaacagct
     2641 ccacaaattc attatgtggc tccttctgct gtgaagcaac cgccccctac tccttcctcc
     2701 aatacttcta ccaccgaacc tcatgtgatt gaaaataaac aagttacttc ggcctcctca
     2761 ggtcataaaa ttcgttcacc aatggttgga acaatgtaca cagcgccatc tcctgaaagc
     2821 ccaccttttg taacagtggg acaatctgtt aaagcgggag atactctatg cattattgaa
     2881 gcaatgaaaa tgttcaatga aattgaagct gatcgtgctg ggaaaattgt cgaaatcttg
     2941 gtaaaaaacg gggaccctgt tgaatatgac caggccttgt ttattataga atagaggggc
     3001 ctgtttatgc tcagtaaaat agttattgct aatcgaggcg aaattgcgct tcgtatactg
     3061 cgtgcttgta aagaacttgg cattcagact gttgctgttc actctgatgt agacaaagat
     3121 ttattgcacg ttcgcctggc tgatgaaacc gtatgcatag gccctgcacc tgcccaaaaa
     3181 agctatctca atattcctgc aattatatct gcggcagaaa ttactgatgc agtagcgata
     3241 catccaggtt atgggttttt atcagaaaat gccgattttg cagatattgt tgagcaaagc
     3301 ggtttccgtt ttattggccc acgcggcgac tccattcgtc ttatggggga taaagtttcc
     3361 gcgattgctg ccatgaaagc ggcaggagta ccctgtgttc ccggttcaga cggtccttta
     3421 ggagatgacg acaacaaaaa tctggagttg gcgcgaaaaa ttggttatcc aatcattatc
     3481 aaagcagctg gcggtggtgg tggacgtggc atgagagtcg ttcatagcga atccaattta
     3541 cttagtgcta ttgcgttaac ccgaagcgaa gcaaaagccg ctttcaataa cccgatagtt
     3601 tacatggaaa aatttctgga aaatcctcgc catgttgaat tccaagtcct tggtgatggg
     3661 aaagggcatg ctatacattt gggtgagcgt gattgctcga tgcaaagacg acatcaaaaa
     3721 gtcgtagaag aagcccctgc tccaggaatc actcctgaat taagagaaga aattggcgcg
     3781 tcagtgatta aagcttgcaa agagttacaa taccgaggtg ctggaacctt tgaattttta
     3841 tttcaagatg gttgttttta tttcatcgaa atgaatacgc gtattcaggt tgagcatcca
     3901 gtaactgaaa tgatcactgg cattgattta attaaggaac aaatcaaaat tgccagcgat
     3961 attcctctga cattaaccca aaaagatatt aaattacgtg gccatgctat tgaatgccga
     4021 atcaatgcgg aagatccaag aaattttatg ccctctcctg gaaccataaa attactgcat
     4081 caaccaggag gcccaggcat tcgttttgat tctcacatct acagcagcta tgttgttcca
     4141 cctaattacg attccatgat aggaaaatta ataagttatg gggcaacaag agcagaagcg
     4201 attgccagga tgcgtaatgc ccttgatgaa attatcattg atggcataaa gacaaatatt
     4261 gaattacacc aacgtattct tcatgacaaa tcttttatgg aaggtggcac aaacattcac
     4321 tatctagaaa aaatgctcaa ggattcatag cgtgtggttt caactaaaaa tagaacattg
     4381 tcctaatgac aagattgaag aaataaccga ggaactcgaa gaatgcggtg ccttgtccat
     4441 cacactcacg gacaaaaatg acaaccccgt tcttgaacca gagccaggga caacgccttt
     4501 atggcctgaa gtaatcattc atgccctatt tgcccaagca gaagaggcgc aatacgcaag
     4561 agaacaatta gttgctaaaa gaccatctct gcattgcagt ttggaactgc tcgctgataa
     4621 aaattgggaa agagcctgga tggatgattt tcgacctcaa cgttttggta accgcttatg
     4681 ggtctgtcct acttggctcc ctcccccgga acctgatgca gttaatttaa tattagatcc
     4741 tgggttagca tttggtacag gcacacatgc gacaacctcc ctatgcctaa cttggctgga
     4801 gcaggctgat ttaaaaaata aatcaataat agattatggt tgtggttcag gaatactctc
     4861 actcgcggct atcaagttag gagctaaaca tgtctatgct gttgacatag ataatcaagc
     4921 cttacaagcc actcaaagta atgcacatgc caaccatatc actgagagcc aactctctat
     4981 cagctccccg gaagcattgc aaaatcctgt acatttagtt atcgcaaata ttttgttggc
     5041 cccattaatc agtttaaagg aacgttttca tcaactgctc ccgtcaggcg cgcatttggt
     5101 aacatcagga attctggaag aacaggctcc tttgctgatt gacgcttatg attccgcatt
     5161 cactcatatt gcaactgaat attgtgaggg atggtcgttg ctggttttta caagcaaata
     5221 ataactatat cactaaggta tatgtaaatg gcgaaaaaac aaatttactc ttctttcaag
     5281 ccacgacgag cactactgag tgtttccgat aaaagaggaa taagagaact cggtcaggcc
     5341 ttacatgacc aaggagtgga attgatagcc actggaaaca cagctgcgat cttaagggaa
     5401 cataaattac cagttaccga tgtcagtgag tgcacgggat ttcccgaaat aatggatgga
     5461 agagtcaaaa cccttcatcc ggccattcat gctggtttat tagcccgcgg agaacaagac
     5521 agcccagttt taagacaaca tggaataaaa cctattgatt tactgattgt caatttatac
     5581 ccctttgaac aagtcattaa ccacccggac tgcgatttta ataaggccat tgaaaacatt
     5641 gacattggtg gaccagcaat ggtgcgagcg gcagctaaaa atcatgccca tacgtatgtc
     5701 atagttgatc caaatgatta ctcaaaatta attcattatt tacaagatca gaaagcacct
     5761 tcccattgga attttgcgtt agctaaaaaa gcatttgccc atactgcagc ctacgatgcc
     5821 gcaattgcaa attatttaac cactttggat aatgattatg tcccaactgg atttcccgac
     5881 atactaacct gccaatttaa taaagttact gatcttcgtt atggtgaaaa cccccatcag
     5941 caagctattt tttatgctga taaaaattca catccaggct ctctaagtac agcaacctta
     6001 ttacagggaa aacaattatc ctataataat attcttgatg ccgatgcagc tctagattgt
     6061 gtgaaatcat tttccaatga aaagtcggtt tgcgtcattg taaaacatac caacccctgt
     6121 ggcattgcgt tatccgacac atcacttgat gcttacttaa aagcctttca aagtgatccg
     6181 atatccgctt atggtggaat tattgctttc aatggaactc tggatagcga tacggcaaaa
     6241 gcaatattag aaaaacaatt tgttgaagtg attattgcac ctgatgccaa tgaagaagca
     6301 aaaaaaattc tggcagccaa agagaacatt cgtgttcttc taactggttt ttggcaacaa
     6361 ggtaataatt ttagattaag tatgaagaaa gtcgatggtg gtctattagt acaagaacac
     6421 gattctcttt ctctggaatc atgtgagcta caaactgtta cccagatcaa acccactgat
     6481 aaacaactac aaaatttaat gttcgcttgg ttagcagcca agcatgtcaa atccaatgcc
     6541 attgtatacg ctaatgattt ggccactata gggattggtg gaggacaaac cagtcgagta
     6601 atgagtgctc gtattggttt gtggcaagca gagcaaatgg ggtttgatcc caaaggcgct
     6661 gttatggcct cagacgcatt tatccctttc cctgatacta ttgaaatagc tgcgaaggct
     6721 ggtatatccg caattattca accagggggt tctatcagag atgaaaaaat tatttcttgt
     6781 gccgaccaac acaacatagc gatgattttt acaggagtgc gacattttaa acattaatgt
     6841 caagcatgta ttaattctaa gcttgactaa cttgaacaaa gagtcgtata taacgtatca
     6901 tcttcttttt aattgaagct ttttgattag cataaatgga ccttttcttg taggactgat
     6961 ggacttgata ttagaacaag gagcaagtat ttctagaagc agattttacc tatagaataa
     7021 ataagaatta taggaatact tactaatcaa ctggcaatca cgcaaaagac aaaaatgcta
     7081 ctaaaggtct aatgcgtcac tgaaacccat ggagtaatat aatgacaact gagcaatacc
     7141 cgtcctcact tgtcaatcgt cttgcaatca cagagcctgc cttggttcca agtggatatt
     7201 atcgttcgaa attatttatt gccccatttt ctacaaatgt tttagttgca gcggccggac
     7261 caatactgtc tttattggaa agactttgtt taagcccttc tttacctcca gtagaggata
     7321 taagagaaaa tatacagcat gaattacgcg cctttcacag taaattagac gcttctaaat
     7381 atccgttgga tattattagc attgctcaat atttattatc agctaccatt gatgaaatat
     7441 taggtaaaag ttatctaagg gtctataacc tgacgacaga gttcaaatca tttactccct
     7501 tatcaagtga tggggcacaa ccccagcagc gtttttttga aatattgaac tacattaaag
     7561 agaggccaaa tcaatttctc gatttaattg aattagccta cttttgcctc attgccggat
     7621 ttgagggtga ataccatctg aaagcagacg ggcgacaatg tcttgataat actattgaag
     7681 atttatatca aatcatacaa aaataccgct ttaataaacc acatcgcttg tttaatgaga
     7741 atcctcttcc caaaactatt aaaaaaaatt ataaagcaac gtggataaca actattgctg
     7801 ctgtaggtat tgtaattcta gcttttgtta ctagccatgt tttattagaa aacaaagcta
     7861 aaactgtttt atttggacat acccaacttg ctatgctgga aaactaatgg acaattcatt
     7921 gcgcgcatta tgtgatgcta taaaaaaaat tctctctcaa ttaaaaccac aagctaatca
     7981 attatctttt attgtcatca ccgggaaaaa cgcccaaggc aaatcggcac tgttaaagca
     8041 aagtaatatg gaagaaatgc ccgtattcag cgaagagcat gccaaactgt attttaatca
     8101 aaaaggaatc atagtagagc ttggtgaaaa ttggcttacc aatagcaaaa cattattgct
     8161 cacaacactt aaacaattaa ataaatgcaa ccgccatctc aagattacag gcttgatttt
     8221 atgcgtggat gtcaatgatt tactgatcgc cgaaccagca caattcactg aacaaaaaaa
     8281 atctcatttg caacttattc aacgtttagg tgttaacctg ggatatcagg ctgaattagc
     8341 tttaatattc accaaaatgg ataccttggc tggcttttct gaattttatc aaatggatca
     8401 tgtcaatgat ctatctaaac ctcttggctt ttcccttgat tgtgtcaacc aattaagtaa
     8461 aaaaattgaa acgttttccc ttcaattcaa tcaattaatt gaagctttgg gacagcaagt
     8521 tatcaataaa atgcacccgg cacgttctac tatcaagcgc agtttgatac gcgaatttcc
     8581 tttgcaatta gccagcttga gagccccaat tcagtcgtta attcagggga tttcgccaaa
     8641 attattccat gttcactcta tttttttcac gagtgccgaa caaggtggag tcagtgtaga
     8701 taggctaaac aaaaaaattc agcatgaata cgctctggtt gttcaggata ctttccctca
     8761 agcgaccaat ttcagatcct attttgtgga aggtgcactg caaacaatcc aggaacaatg
     8821 tatccaggta ccacagacta caaaattttc tcaaaaacca ttcattgctg ttgcagtaag
     8881 tttggctggg ctcagtttat tgacgctgat ctataaccac tacaaaacct ctcatattct
     8941 tgatgaggct agtaaagaat tgcttgccta tgaagcactg aataatcagg gcactagtgg
     9001 tgcacaagcg ctatatcact tatcgcaggc tgcaaataaa atgaatcaca ttacatcaaa
     9061 ctccatatcg ctgcctactg tgcaccaatt aaaattaaac ctttacaaca atgctcaaaa
     9121 tcgtttacaa ggagaatttc ttccctcctt agccaatgag ttagaacaag tcataacaag
     9181 tcctggcaac acccctatta ctcgttataa ggctttaaaa atttatttaa tgttaggaga
     9241 ctcaaaatat ttttcatacc aacccattct ggattggttt caacagcaat ggcagggacg
     9301 tgcaccaggt actacgcaaa aacagttggc tcttttaaaa caaactctta gcaatcctcc
     9361 tcaaaatata cccatcaaat cacaaataat cagtgatgca aggaattatc ttaatgcact
     9421 tccaacaagc tatttatatt actctattgc gaaagaatat ttccctcagg aaaaacaaaa
     9481 gattgtgatt gaaggttttt ctatacccac ggatgaagtg ccaacctatt ttactaaaac
     9541 cggatttacg aaaattatta acgaattacc taaaattacc agcacacttc aatcagaaaa
     9601 ctgggtactt gcgagacagg atttgtctca attacacaat atgctgattc aggcttattg
     9661 ttttgattac gtcacttggt ggcaaacgtt catgaagaaa tcccaaccat tacgttatca
     9721 ggattatcag caaggtcgtc aattaacaaa attaatacag caatccaatt cgttttataa
     9781 attcgctaat cttattcagc aggaaaccaa accggattta accgaaggaa tatccacctt
     9841 taatcaattc attgccaatc aatttactga tttaaattta acaactttat ccagcattaa
     9901 ggaattagat tttagaattt ctgaattaga aagatttatt gcaactttat ctgttgtaaa
     9961 tgatggaggt aaaacagcgt ttaccattac acgctcgcgt tttctcaatg aaaattcctc
    10021 tgaccctgtg agtttgctct acagtcaggc acgacaatta cctgaaccat tgagttcgtg
    10081 gacaaagcaa atagctggtg acacttgggt gatactgatt agagatagtc gtcaatacat
    10141 taataatcag tggcaaaaaa ccgtctatag agagttccaa aactctattg cccagcgcta
    10201 tccttttgac tcgtcacaaa ctgcagaaat agatattgca gattttaatc gctttttctc
    10261 aacacatggc ttattgaaca cctactctga acaatacatt aagccatttc ttgacacttc
    10321 atctccagaa tggaagccta aagctgtcaa tgatttcatc ttgcctattt caaatgaagt
    10381 aattgatgag ttaattcgtg ccaatatcat tactaatatg ttctttcctg atcacagtga
    10441 tgaaagcaaa attgatttta gcttgcaaaa aatctctctg gatcctgtag tttctaatct
    10501 agtccttgaa attggcggca ctaaattgat ggataatcaa acaagcgatt cactaattcg
    10561 cttcacctgg cctcaaaaca acgctaaatt ggctcttgat tctattgagg ggaatcatta
    10621 cgagctcgca gaatatggaa cctgggcaat atttaaatta cttgaaaaag tcaatgtgct
    10681 tgtggatgaa caagatagtt ccagcctcca aatacttttt gaagtgaata gcaactccgg
    10741 ccgctattta ttaaaaacaa acaaccaaat taatcctttt actccaggaa ttctaaatgg
    10801 gtttacttta aacgaccata ttgcctgact atgaaacaaa ataccaaaca cagcattatt
    10861 gctggattat acggaaatgc tttggagtgg tatgattttt tactctacgc cagttttgct
    10921 cctgtctttg ccgaaatttt tttcccttca aaaatacatt ttgtttctct tatagcaaca
    10981 tttagcttat ttgctatagg attcattatg cgcccaattg gtgggctttt gcttgggcac
    11041 tatgctgatc atgtaggacg gcggaaagca ttaatactat ctatgtcagt aatgaccatt
    11101 tctaccgcat taattgcagt attacctgat tttaattcat ggggaattat cgccccactt
    11161 ttattttctg ttttacggct cattcagggt cttgcagtgg gaggcgaact gcctggctcg
    11221 accacctatt tgattgagca tatgtttcaa caaagccggg ggctcgctgg cagcctcgtg
    11281 ttaagcagcg cttttttagg tatttttatt ggagcgctta ccgcatcaat attcagcaca
    11341 ttatttacag gtgatgctct attaaattgg ggatggaggt atgcgtatct gttaggaggt
    11401 attttaggaa ttttgggcat ttatttgcgt attaaaagct ttgaatcacc cacatttcta
    11461 aaggataagc atactgctga acttcctgca aaagtggtat tcacgcgatt taaacagcaa
    11521 ctgtttttat ctgtgataat tactagcatt cttgccatgt ctaattatgt actgatcgcc
    11581 tatgtcacaa cttttttagt caaatcagag tattttctct tgaaagatgc gttattaatc
    11641 aatttaattg cccttgctct tttgacagtt ttaataccgc tgatgggatt tctttctgat
    11701 gttgtgggac gcaagcctat tttcttaatc ggtttatttg ctctttgcct actaatcttc
    11761 ccattttttt ggctcttatt aagtggtagt tggtggcaag cattatgcag cgaattatta
    11821 ctggcgatag ctttagcccc aatcaatgcg acagtaccca ctattattgc agaaatgttc
    11881 ccaaccagtg tacgggcgag tgggatttcc atatcctata acattggcca ggctttattt
    11941 ggcggtacca tgcctttggt tgcgtttacc ctgattgaat taagcagcaa taaaatggct
    12001 ccggcctggt accttttcgt atggaccctt atcgttatac caataactat ttccattcgt
    12061 gaaagctatc aaaaggagct tacttaaggc attagattga gatgaaccac caaaatcact
    12121 cttgattgaa taagcaacaa taaaatttaa atcagtcttt tagcataaaa tatccgagta
    12181 ttaataccca gaaaatagta gctgttctcg cgatggcgtc agcgatagca gaaaataaga
    12241 gcatcgtgac atattaataa aaatcttatc cgcttcaacc cacttttgca ggcaagcttt
    12301 tcacgttggg atcttttgaa taagcatcaa gaatatcatt caccaattga ttaatcattc
    12361 cctctctctc atcgtcttca attaatccaa ctttttgctt catgcttgcc aggcgagcct
    12421 ccaattcttt agtcactgta ccatttggga acagtgcagc taaaagccgt cgagcttcac
    12481 ttggtcccag atgcctatac aaaagatttc gtactgtggc atcttctgca gtagtactaa
    12541 atgcccataa ttcgaccgga cccaatgtca aggttaataa ttgtacgttg atacctgttt
    12601 tagtagcaaa ctgagcaagg aaagtagcgc caccttgtct aggaccatga actcgagtac
    12661 gtaacgcaat cttggctgtt tcagaaagtc caaaaatttg actggtcttc tcgacagctt
    12721 gagatggtcc tgcatccata acataaatag cggtagcaaa atcaatcatt accggatcaa
    12781 agtcatctac tgcttgtgat aataaggaaa tttgcacttt ccattttcgg ccttcacgca
    12841 tatcaataat aacttgctca cgaacagcag acgattttgc cgtgcgatgg aattcatcat
    12901 aaacaatacg cttatgatct tcccgaatct ctaatactct ctgtttgtga tactctttat
    12961 attgttccgg tacattattc aaactttctt ctgttaaata atagtgcctt gccaatacat
    13021 atctggctaa catatacata actgctgttt gtctatctgc cgcatcacca ccacttttcg
    13081 ctacttcatc caaatcaaga gacactactc tcgcgtcacc aatatcaaaa ctagtgaccc
    13141 gtgataaaat aggatattcg cgaacagctg atgaaatcat tcttgaaaaa gcattaatta
    13201 gtgattcacc ggttggtgcc gtaatctttt catataaatc ctcaattgat ggtgttctac
    13261 agatagaagc agcgtccgct aataacggca ttgcatatct ttgcgctaac atcgcttcat
    13321 gaacaaaacc tgctgaatag agagcgtccg tcacttccca ccaggttgat ttggcatctc
    13381 ggacaaagcc tatttcctct aggatgctat cgataaattc ctctattcct ggagcataag
    13441 gtgtcggatt gaattcatca gccatgcttt tatataattc atctaccacc atacccgcca
    13501 aatcggccat accatcatat ggctttgcag cacctaatgg cgtagttagc aaggtcatga
    13561 agttgactaa gaatgatcgc tccaaagcag ttggataacg acatcccaat tgggtatcaa
    13621 agggattaat ggaataatct ggggtcatcc ttaatctatg ataagcaacc aaatgacgct
    13681 tggatgcagg gagagcctct tttagcaatg aaatcaaacc gctacttgag ggacctatat
    13741 caataattgc tatgcgcggc aaacgcatta aaccacctga aagacaaaga gctaaattca
    13801 gtgcatttga caacacagat ttaccagaac caggacgagc gtaaaccaga tcaatccaag
    13861 tagtttgttc agttgagcct ggttgaaatg gccacggctt accatctggt gttctaaaaa
    13921 gcaaggctcc agttttccag ggagatgctg gtcttgtaat gggcagcatt gagataacgt
    13981 ctgacaaagg agctactgat gctactgctg agctgttcaa agtagtagct agcatacttg
    14041 acacaaagcc tgcaaacgga tccccacaaa tctctgatac atcagtagaa ccccaacctt
    14101 caatagcctt tactaactca gagcttcttc gccgcaacaa gggaatattg ccttccgggg
    14161 cccaggttgt tgcaacgacg cgtagtctta caatcgcctc atccgtattg agttgtatat
    14221 atttcagtag attaaccgaa tcactgatta aacgattctg tgcggagcta aagctcaata
    14281 tcgcggccaa gagccccttc aatttaattg tgcttaatcc ttcgctttct agcaaaaaag
    14341 aaattttcca gggcacatgc gaaggaagaa tacgagaaaa taaactaata aatggtctta
    14401 catcctttgg aaacaaatca atataggttg aagcataaat tttatcgccc actcggacag
    14461 tgcgtaaatc aataatctct gcatctcttg gcaaaacctg cctggacaac ggcggccata
    14521 ataaatcaga aggatcccct tcaaaattat tcagctcgcg aacaggtatt ttatctcctg
    14581 gtaaagttgc gcgccaatca tccgcagtat aatcaggatc acctgtcata cgaatggcat
    14641 gtactgcatc gtgcacctca agcaacttag ctatcacatt cagggcatcc aaatcattta
    14701 gaattgcacg gacataagca tcatgtgtat ctcgtatttc tggaacagcc gcaaaaatcg
    14761 tttgtgtgtt tttaaatgga ggagcctttg tatccttgat catcttcatt tttgctttgt
    14821 tcgcagcttt taactgatcg ctcgccaagt taaatggtct cgtatacaaa acaaaataaa
    14881 cacgttcctc tgcacaatat tgtgatagat aattaaccct ctcctcaaat aaatctctta
    14941 aattaagttc aagacgactt gctgttgctt ccgcagggtc ataaatatcc ttgatcattt
    15001 ttactatgtt ttgcttatca tggctaaaat aaacttgtag cgcatgacca ggtctaccca
    15061 tagcagcttg aaatgagttg gctaaacctt caacgagtcg ttcaaactct tcagggcctg
    15121 ccaatccagt aatcccttca atttttaata ccgacagtaa tgatccatca tgattaacta
    15181 atacggtagg gctatccgcc gtttccaaat ctatataaga ctctgtagtt tgcttcaggg
    15241 aagtacttaa ccaggcaaag aaagtatcga cgccttcaaa aaacgattct gaccaatttg
    15301 ccatcctttg tcctcaatta ttaatagtac tcatactgtt taatctgcaa tttcttctaa
    15361 agcactggca gcccatttct ctatttgctt cctgaatttt gccctagagg gtaatagcct
    15421 tatattttta aaaagtttct ctcttatttc ttcactattc acaccagaat caatcattaa
    15481 ttgcccaaat attgcagggt cacttgtgcc ttcaccaaat ttttcttcaa caatttgcga
    15541 gcgaaattta aagcttctat aaatattctg tgcactggat ttgtatccgg tatcattggt
    15601 aatagcgcaa aaaagaacat ggcataagct atttagttca gcctgggtta attctggcag
    15661 gtagatcaaa gtacctccac cataaccacc aactcctact gactcaacaa aaaagcattg
    15721 cgcacaaaaa caacaagctg taactaaatt tgataaccta ttgtttgtat aatcaccatc
    15781 tagattcacg atgtcttggt ataatcgggc ttggaacccg caaaattgac aagtatacct
    15841 atctcgctga aagatttttt gctcatagga tttgaatcgc tcatcaattt ttctggcaga
    15901 atataaacgc caggaacctg gtgaagcaat aagttttagc tcacatcgtt gttgattatc
    15961 tgccatattt tggttcacat tcagtcaatt ttcaaaaagt tatgtgttct atccttaact
    16021 actgactaag ccagcttaaa gatatttgac catattaata gagacaatca tttaaattta
    16081 ctatttaaca caataaaaag cctataaatt aaattgggta aagcgttatt ctctttaccc
    16141 aaataatata aataatctct ctattaatta gtcttatata ttgttccagt aggcccagca
    16201 gttttaccac cactaccacc gaacatagtt actccggcca ctcctaaaat agaaggaagg
    16261 aataataatg cagcagcgat aaatatcaaa gctattggtg ttccaattgg aatttgagta
    16321 gggttatcct tgtgctgctt aaatttcata atagcaccaa tagaaaaacc caatccagcc
    16381 aagtaagagc ccgctgtaat caatttagta agacttgtaa atgaacccgt aatttgtgat
    16441 gccattttac ctaacgatac acctgctgca gcatcctgac aaattagagt caataaagcc
    16501 aaacaagctg cgcttactag ccagtgttta taacctgatt tatggataac cttaatgttc
    16561 acaattctct cctgttttta aattagctta agtaccatat aaagtattgt taatcatatc
    16621 aactgtacca acaatattga ttgccaatat tcccccaaat acatgaatca aacctttacc
    16681 tgtacctcct ggaggttgcc cctgcgaagc ggaacgcgca attaataccc accctcttac
    16741 aaatgcaatt agaccaataa cccggataat catcaccaac ggcctgccta ctagactgcc
    16801 actaccaaat aaggctgaaa tagtatcgct tccactactc acacctcctg agtaggctaa
    16861 tggattgcta taaccaaaag tcgtttgcag tacggaggaa accaatgatg ggaaatagat
    16921 aagtaatgca cctaccatca aatacatcac cggctctttg atactcgtat tactcgacat
    16981 cattgtcctg gcttctccat aaacctttaa gctataaatt gccttaaata taaaagcaca
    17041 cccaatcaaa taagcgcctc cagtaatcag cctttgaaca ggctccaact gattagcaat
    17101 attagctaat atgttagctt gactcactat ccagcatgct actgtgccgc cagtacaagt
    17161 cggatcagcc atatacgcct caactatctt cttgactaaa cttaatcact tgtccagaac
    17221 ttgttaaaat acgaccttgc aaagaatcaa ttaatttaac cattccatat ccaggaattt
    17281 ttgatccctc tctaactgta agtgtagaac catttgagcc aattagccaa gcccgacctg
    17341 gaattactgc ttgtatgtaa taaataattc ttgcttgaac tgttggacga gacactttta
    17401 caactttctt aggtgttgta cgtgccatta agacattaat cacttctgat tgtctagcaa
    17461 tttgatttga catattacca ataatttgat tcagatttac aatttgagca tttaaatttt
    17521 tgatattatt atttactgca ttgatttgct caccaagggc attgacttca gaacgaagac
    17581 tctgctgtgt catttctatt gctgaaactt tcttctttag atcggagtca tcttcaataa
    17641 tagtcgtagg ctgaacttgt tgtatgggag tggtggttgg tattgtctga acaggttgtg
    17701 gtgttgctac ctgtgtcacc ggaggaatag caggctttga ggttacttgg ctggatttat
    17761 ctgagaaaaa catccagcca attattttat acatcaccat tataagtaca actgccccaa
    17821 tagcaataag agcatttcgc ttgatatctt ttttctttgt taatccctct ttcccagatt
    17881 gaaacgaagc ttctgaatcg agatccgatt caccagcctg atccatatcg tatgagtcaa
    17941 gttctgcaaa tttatattca tcattatttt gatcgtgctc tgccatcatt atccgccatc
    18001 aaattaaatt gttgtaacat cctgggtaaa taaaataccc aaacctgtac cagaataaac
    18061 ttccacagtt gttggtgtat taaacaattg ttgcgcctgt tgactccatg cctttccaac
    18121 ggtagccaat cctataaccg cattttccaa agttgagcga ccaacaccat tggctacagt
    18181 aatattatta ccaccaccag tgcccccaat ggtgattgtt gtatttgccg actggaatgc
    18241 atttccaaat ccttgtaaga aagaggaagc aaacagggaa ccatatcgca ttaaatagtg
    18301 atgattagtt ctactggata gggctgttct tgccgtgttt ggatcaattg cataagctga
    18361 gatggatatc gttttttctg ctcctggaat tgacattgta ttaaaagtaa tcaccatctt
    18421 gtcggcattg gacggtaagt taaagcttcc tattaatttt gagcctttta gcttaccagt
    18481 aacaatagtt gccaatattg gacctggctc atcactgttc acagcggtat ccagaaccgc
    18541 aaacataata tcgcccgttt tgataatagc ctgattctga gcaccactta cgccttgttc
    18601 tactggttga ttattagcac ctgcaccagt gcctgctcct ggaactgagc tttcaccacc
    18661 agatgttttt gtttcttctg taccttctgt atacacctgg gtttcaacct tcttccagtc
    18721 ttgaaccaac tgagtcgccg ctgtgagcat gtcagaagtt ctttgttgaa tttcttgctg
    18781 atatttttgc tcagccaatt gttcattttg tttttgcaaa atggcctgaa gttgctcagc
    18841 acttgaggca gcatcactgg ttgtcggtct tggagtagcg ccaggaattg agggaattcc
    18901 tgttatactt gagtcaagtt gttgcaaatc aggcccttgt aaccctgcaa cggctgattc
    18961 ttgtggagaa aatccagcat ttctcagctc agctgaggta tatccggctt tttttagctc
    19021 atcagccgaa tatccagcat tctttaattc tgaggcacta aatcctgcgc ttttcaaatc
    19081 tgaggcgcta taacctgcat ctttcaattc ttttgcgcta aaaccggcat ttttcaaatc
    19141 cgaggcacta aaaccggcat ccttcaactc ctttgcacta aatccagctg cttttaattc
    19201 agctgcagag aaaccagcat cttttagatc ttttgcagaa aacccagcag cctttagctg
    19261 agccgcagtg aatccagccg cttttaattc agcagcagaa aacccagcgt cttttaaatc
    19321 ttttgcacta aaacccacat cttttaattc ctgggcggag aaacccgcat cacgcaattc
    19381 ctttgctgta aaaccggcat cttttaaatc tttcgcacta aaaccggctg cttttaattc
    19441 agctgcagta aacccggcgt ctttcagctc ttttgcagta taaccagctg ctttcaatgc
    19501 tgcagcggaa catcctaatg tttgctttat tgtcaaagca ctaacaccag ctgccctcgc
    19561 tttttttaat gactctacgc tacagtctgc aacacggccg tctgcaataa tggaagcagg
    19621 atttaatcca gcatcttcca actgctttgg tgtaaatcct gccgcaagca attcttgcgg
    19681 tgtaaatcct gccgcaagca gggattgcgc atcatatccc gcagctttta aagcttccgc
    19741 actgcaacca ttgagttctt tgatgcgttt tgcagataca cccgcagaaa ataatttttt
    19801 cagcttatca ggatcacagc cagcggcctt tatttccgca tcactcaaag gagttgctgc
    19861 actgatttga gctggtgtaa atcctgcgct cgccaaatcc gcatcagtga atccggcagc
    19921 tttgagggcc tgtgcgctac atcccgcgta ttgtctgatt aaagcggcgc taactcctgc
    19981 ttccctttcc tttttcaaag cctccacatc acaaccagca ttcttcacat cttgcggagt
    20041 aattccaggc ggaagctctg cctgagcagc tttgatttga gcattagaaa atcctgcctt
    20101 tgccagatca gctggtgtaa atcctgcgtt taacaaatct cttgcagtaa atccggcatc
    20161 ttttaattct gcagcagaaa aacctgctcg ttttaagtcc gcagcactaa aacctgcatc
    20221 ctttaactct ttagcggtat aacctgccgc tttcaactgc tcagcggtgc aaccacttat
    20281 ttttctaatc gcggacgcac taacccctgc ctgacgtaat ttggccaatg cagcagctcc
    20341 acatcctgct ttgcgcacat catctgcagt tataccatct ggtaagccac ttgcttttgc
    20401 aatttcagca tcagagaacc cagctccttt taattccccg tctgaaaaac ctccatcttt
    20461 catttcctgg gctgtaaaac ccgcattacg taattcacac gcatcaaacc cacaatttcg
    20521 cagtcgacct gcactataac ctgcttcttt caactgcctt gcattataac cagccgcttt
    20581 tagctcttta cactcacaga cctgctctaa ttcttgcaaa ccgtatccac tatcttttaa
    20641 ctgaacacag ctacaagccg ctttcaaatc agttaattga gctccctgat ccataacttt
    20701 cgtaatcaca gatttgctac aaccaccatc ttgtaactct tgtatccata aacttcgttg
    20761 aggaccacct aactcttctt gagccaaagc agcaaaaccc actccacttt gggaaccaat
    20821 aacaccaact ccttctccta gtgcttgggt gcggataatt gttggaatag cactcccacc
    20881 tgttttttca gcgacttgtg cctgggttat attttgttct tcttgcaatt tggcatattg
    20941 agcagttgga tctaaaactc ctggaataga ttgaattcct cccggcactg tagaaacttc
    21001 agctgcagca atcgaactgg tagtagcgct tcttatttta aaaaagccta ttactaccgc
    21061 gatgattaat aaagctgcag taaaaacgat aatcactcga gtccgagtat tggaaaacag
    21121 tgactttaga ttttcttttt tgctcgccat ttgcttataa cccttctacc ttgagttgca
    21181 tgacttttcc atgccaagac actaataaaa ccggagattt ttgcatttca taagcatgcg
    21241 ttccatctgc gctagtcata ctggctaacc agcccggcga caatatagtg agattcgttc
    21301 taacatacat tttttcatta gataaccaag ctcgagcatc cccaccacta accacgagac
    21361 gcctgctccc tggcggagga acaccttcta acacatgaag aagtaaatca ttcgctgatg
    21421 ggggaatgcc ttcttcagtt ggcatacttt tagcgttggg gccatagcct tgaactctca
    21481 agtctactcg ataatccaca gctttctgac cgggtataag agtaagcatg acaggggtat
    21541 ttaatcctct taaccttact gccaagttac cataattata tagcttcgta gcttgaatca
    21601 ttaaagtatt actggtttta tcccattgaa tattaaacga agagggatca cccaaatcat
    21661 atgcagcaat aggccaagga gctcctgttg aatccaggaa cactaaggaa gacacaaaac
    21721 cttgtgataa tcttattacc ggcggtgttg agcctggaga cagattaaca aattgtgatg
    21781 tcgctgtagg tttaggtgga gtgcctgccg ttgaagcttt ggcatactcg gaagtttcat
    21841 aaatttgttt taatttgact acttgctcag gatttaaggg atataagttg cgtgtcatat
    21901 ccttaaatgc cttcttatca ataacttcgg catcatcttg actaattatc tgcccatcac
    21961 cgcccgcatt tgctgtttga tttgccgccg gagcattagc ctgaccggat tgattaggtt
    22021 gttgcgttga atcagaagct gcattatcac cgccatcacc ggctccagac tgtacatcag
    22081 gggaagggtt ttgtgataat ttctgttgta gcattcgcag ttgctgcaac gcttgctgag
    22141 catcatctga ttgatcagcc gcataggtac tgcatgacat actgaatgtc aagcctatca
    22201 ccaaacaata tttacataat tgatcatatt tcttcatcac taggataccc caccgctggc
    22261 aggtccaaca acaaattgtg aaatacctat ccctctaggc gaattcaacg ttgaaactct
    22321 agttattaac attgttacta cgttattttg ttgtgtaaat tcactggcac tttgataggt
    22381 gactaatatt ggcatttgta ctctccagga atacctacca ttgagcactc ctttttgcaa
    22441 aatgattggg gctcgggttg ctactgctga cacaactaat tttttcgctt taaccgcatc
    22501 aaggttattg gattgctcta atgcacctaa aaattggtcc cagccttccg cagtaaaaaa
    22561 gccggaagaa gcctgaagct catcgcgata atttacgaaa ttataggtaa atgcggcaat
    22621 tgctgcctga ttagcccatt gtagtacagc ggagtccgat tggtttggtt catccaaagg
    22681 aaataaaggg gtaatacgac cgttaatact tgttgcaaag tactttggag caggtggatg
    22741 agtaatcata tagacaacaa gcgatgccaa gaccacattt accacaatag caatcaataa
    22801 cgccaaaatg actttgcgtt gactgtcttt ataaaactta tttcttaatg caactacagt
    22861 taaggcatct tcagccataa tatcctctaa aaattattgc ggtataatcc ttcgtgaatc
    22921 ttcatctggt ggatccggcg gaagcaatgc ttcagatgac atgttaggag tcagcattgg
    22981 cttaagctgg ataggaggag ttatgccgct ggtcgcataa aaatctcgct caggttcatg
    23041 tatatgaaca taataaatca acccagacaa accacaactc aatccaagag aaagaattag
    23101 cagtgttaag ccacgtctgt aaatatggac attaaaattc ttatttcgtt ttattaaagc
    23161 ccatgtttct cgactcattt ttacctcaat tatgccacag cccgcctaaa tgtgatctcc
    23221 accctggcat taggtgatct atcaccacca agagtataac tagttatagg tttatcacta
    23281 cctaaccctt gtgtaaagat gattctgcta tcaaccccct gagaccatag gtattcactt
    23341 actactcttg atctcgccaa tgtcaatgcc cgttcccttt ttactgacac atatttacta
    23401 ctgtaactgg ttaccgttat agcaatttta cgaaattgtt taagaaacgc cgcgatctca
    23461 tttaaaaggc tataggaagc ccaatttaac cgaggggatt gatcagcaaa cagagcgcta
    23521 gcaggaatac taattaaata attttggcct actgatgcaa ctttaatccc ttttttattt
    23581 aaatcagtca tcatttttat tattgttgca tcgcatgcac catcaacacg gcatggtaat
    23641 ttgggcactt cattctcagg gatataacca gaacgattgc aagcactcag caacaataag
    23701 aataataatc cagttgtttt aaacaataca tagatgtagt tagtacgaag tgatctcacc
    23761 tgttatcccc atctttgaaa atagactaat tgtgttatct atgtctaact atctcaaaca
    23821 ttccatttga taataaaaac atacaaatca ttacgttaca atgaatttaa taaaattgcc
    23881 atttttttaa ataatttatt tatttaagat ggggttgact aattaaggca aatcaacata
    23941 tgccaaatta atctatgagt aaactgataa ttaccctgtt aattcagaat acaaaaagtt
    24001 ctcccaaaga tgaaccacac agaatatcaa cctgatagat caaggtttaa agaaacaacg
    24061 tctctgcccg gattataaaa aactcagagt agcttacata tggagctctc ttcttgccca
    24121 atagcacagg aacacttcta cctccaattg ccgactgagt ctttatgtta attcctctgc
    24181 agccttttta ttggctttct cacgttctgc ggaaatttta gctgataaat ctcttataac
    24241 accagtaagt tcttggacat caatcacatc cctttcttca ggaggatagc tagtcgctat
    24301 ttgaaaatct ttgattaatt cattagctac tgtaccagcg tatttgtctt tagcccctgc
    24361 caaacgctca atcgttatca tttgatttcg tgtttcatta attggtaata atggctcaga
    24421 gaaaacttct ttgtcagaga ctaatattgg aggagcatta gggtcaattc gtagtttgct
    24481 gaaaatagtt aatgccccct caacttcttc ctctacaata tcttccacag gctcgggttc
    24541 attttgacca tgaaatgcaa ttaaagcagc aacgcctcgt tcaattggct ctacgatagt
    24601 agattctttt aatgccttac tgatgagagt gatttcttcg ttctcgactg ccttattgat
    24661 actcaagtca ccactttcaa gaatgctctg gaaagaagcc aattgtttct gtagcttcat
    24721 taaataatca tctggcggcg gctctacttt taaaaattga ttaattttca attgcttaac
    24781 tggttttgga ttggcataaa acatgcgagc acgaacaatt ttggatttga aaaatatatg
    24841 cgcctcacct tcagtttgct cttttaaatc gagaaggtct acccgagctc tcttttcgaa
    24901 agaagagctt ttagtatcca tatagctgtt ggctatgctg gtttctttgg tttgaaacga
    24961 atccactttt gtaacataag cttcgccagc tgttttagtg aagaaatccc aggtttcggt
    25021 aggatcttcc agtttcatac atattttaat gtttgtgttt gcaccaatgg aagctgcctc
    25081 ttctttggat gctttttgga atgcaggtaa gtcttgtcca gcaaaaatag cagaaaatcc
    25141 tagagaacga gcttgcgcag gcactaccgc aaatccttgt actgcgtaat aaccgtactc
    25201 atcaagaatg cacatataag gagttggggc attcgtgggt tttcttagaa taacatcacg
    25261 ataatcacct tctacttctt cacctaaacc tgccgccatc atagctttta gtgaagaaac
    25321 aataatcttg ccaaggttag ataattcatc cggtgatttt tccagtgctg gtaataaaac
    25381 cactaatata cgtctgttga gtaccacatc tttaaaatcg acttctgcta gattggtacg
    25441 aataatatga ccataagtat ctgccagaga tgagaaactt cttaccagct gcatggtgat
    25501 aaaaccatgt tgctccaaaa cttgggatac ctgtttcccc tttttctcct tattataccc
    25561 aggcaaagta tttaaatagt ttcttaaagg atccgttacc aattttggaa tagtttcaat
    25621 attgacactt tcttgatcat ctctgggaaa aagtttatcg attacaatag attccaatct
    25681 ttgcagatca aagtaattcc gaatagtatt tgcatcaagg agaattgcgc cttcatcgcg
    25741 catatacacc aataaacgca ttaatgcttc tacgaaagcg atggctcgcc ccttccacat
    25801 atcaccatca gatgattggc ctgaagatcc catcaaacta actaccaact gagttagcat
    25861 actggaagaa ccttggcaga atgggtttag tgtattggaa agtctttttt cctgcggacc
    25921 gacaatatcg cgcgctccag tcataaagtt gattaagagc aaatcatctt cacgccccat
    25981 gctccttacc atagaaaaga ctttagcata tagagagtta tctcctttac catcaacgta
    26041 gataaacccg ctcccctgta ccagggcatt atatgacaga gaaaccaaag tttctgtttt
    26101 accactacct gttgaaccga aaattaacgc atgggtacgc atatcatcgt tagcaaacca
    26161 aagttcctcg ccggtctttc tgtcattacc aaaaaaggta atacctcgag ctatattcgg
    26221 tttattaata cccggtttta aatcgttgta atccttcgca cgcgaaattt gaggcaatcg
    26281 aaatggcaac ttttgctttc gggtatagct ataaagaaaa aatatgcccc ccataatcag
    26341 caaaaatgtt gctgcttcag agaaataata tgaaacagct gctaatgaaa taagaacaat
    26401 agaaatgttg gtcggatctg caaaaaaatc tgccaaccgc tgcgtaaaag tacgtgtatc
    26461 cctgagtaaa agggttggat ctaactcatg acgagaatca ataccccgca tcatggctct
    26521 aattcctcca tttgacgtgg tgttaatctc acctctttta ctgcaatttc cagagctcta
    26581 atcgcttcat ctatcattgg caccaaagag cgtcttccca tttccttttc agctttccaa
    26641 tgtgcgaaag ggcctgcaac ttcagagtat ggagtttgcc tgcctacaca attcaacata
    26701 taccataaac gtctgtcgac tggtttgagc cataaaaact ctgagctggg aaccacacca
    26761 tcttctcgtg ctgcttcaag caaagaagca atcactgtga gcacataggc atgtttggct
    26821 actacttcct gaaccaactc tgagttttgg tattttttca ttactggcct tgcaacagaa
    26881 aaatcaggtt tcccatccac aaatgtttta tctaaaactt taaggatatt atttgctgcg
    26941 tctctatctc tattcattct tgccataaat acggcagaca atgcataagc ctgaggagag
    27001 caacgttcaa aaccatccca ataagggccc aattgcatgg taaatactcg tttggcatcg
    27061 ccacgccgaa tccccgctgt catctcttca ccaggcacag gattatccaa taacgcgtcg
    27121 tccttcctca ataaattata ttttcgcgca aattccatgg gtgttaaagc cattgcccaa
    27181 ggccctttat tgacgtcttg actaaccaaa tcctccttaa ctataggcat gatggcaggc
    27241 caattaaatt gttcctgagc ccgcaaactt ttcatatcat aggttttgcg atattttaaa
    27301 gtcacatttg agttataaag aacaaaggct aacactacca ggatacaaat aaccgggtaa
    27361 cgcatataat caccgaccgc acgcatcaca gtcaccattt gatcccaatt gacagtgtta
    27421 ggatcaagtg tttgcatcag ataaatttga ttggccaaga gttgattatt taaaaacaaa
    27481 ttaactaatc ttgcttgcca gatattgata gtaaatacaa aactaacaat gtactgatga
    27541 gccagtgccc aaacaaaata agcagtaata aataacagta tgacgatcca tactggcgcc
    27601 atggaattat ctgaacctga ttgctgctgt tgttgtgcca tctctatata caattagtta
    27661 atagattcta tatatcaagt atatactgct cgcagctatt tttgcgagat tttcgtgtac
    27721 tatatcaaaa tatgaataca aggatgatta aataaagcgt aaagacagtt agagtagaaa
    27781 taaattttta gcacgctcga aatcattata aggaaaaccg aaacggccta tgcattttta
    27841 atgagacggc cattggagta tcgatatacg ccactcacat gaacaaagcg gctgatgttt
    27901 tccagactga tggatttatc tttaagcgtt aataacggtt tacctagttt atctaccgga
    27961 gccttatcag cggataaatg caagatatct gattgactga tgtaaattgc cacatacgct
    28021 tcattttgtt tattttcttt tgtttttaat atcgctttta tgtcttcttc atctgcgtaa
    28081 ataggtcgtg aaatcatttg tcggggcaaa ctccccacta ttttctccca ggactgaaga
    28141 ttgcttccat ctgaggaata taatgaaaca tatatttcct gctgaccact ccttaaggca
    28201 attcgatttg caagatgaga ctctgctttt aacttggcct gactagcggc acctatccgt
    28261 tttaaaaaac gatcgcgaat tgcaattaac tttttaccaa ttacccttaa gaaattggat
    28321 ttatcccacg ggcctttttc aatagcatcg tttaaggcct ttagaatagt ttctttttgt
    28381 tcatccgaga gttgatcttt cataacttca atacttatgg gaaccaagaa ttaggggaaa
    28441 tataaagaaa gtataacagc atttgtaaaa atcactgaaa tgaaattaat ctttcaagat
    28501 gataatttga aaccacgttc ctcatgactt ggttgctcag acgacttttc agattcagca
    28561 gcaccaaaca ttctttctgt ctttgttgtt aaaatcggtt gatttaaggc ggctctctct
    28621 acttttgtat tgagctctgt taacacctca actgcatccg tgttatcctt ccctccggga
    28681 ttaaagctaa cagcctttgc aacagtcaaa atgcaatcct ttaccggtga aataatttca
    28741 ctatagacac tacctagagt agcatcagtt acatcaggtt cacctatctc tttaacacga
    28801 ggtggcgtat aaaaaccaaa tggatttcgt gcactgtcat cagtattatt acccattatt
    28861 accactcctg agctaaatct cttactttac atatatcaaa atatatcttt caatatatct
    28921 tggtccaata aaactttcta attctttaca aacaactaat catacattaa ctcatccagg
    28981 ggagtataat cacgacttgg acccccttga ataagctcat tcataacatc cgtcatacat
    29041 aacaaacgta atacattcgg agtcacttca tcaaaaacca ctctaatacc tagtaacgct
    29101 ccttcagact cgtcataagt cgctcccaaa ccataaccca aacacaagga acacaattga
    29161 tccgctctct tggcaatagc cggtagcaaa cccgtttctg aaaaaacttc atcaaaactt
    29221 acaaatctat cgttcaggta aaatttcgca ttcatgcttg ctgcaatctt tgccatacac
    29281 ttgctaatat ctcgctccat ttatctccac caaggtctag cagatctaat tgaaccagct
    29341 aaaaaacttc tgagccagcg taaaaaaacc gggacagtga aaccataatg ttcaattatc
    29401 ccaaaaaaaa ttgcagaaac taagaccaaa acacctgtcc atactcttat atgcattaga
    29461 aataaaaata ttgggaatgc ggccctcgca tcaactacaa aaaaccgcgc gcttctcgct
    29521 gagtcacgcc agtggctcgt tgctgaaaat ccacctgcca tttttaaacc tcaaaaaatc
    29581 atcctttaaa aaaagtatat acttaaaaaa cagcttgtgc acgttgatac tttcaagaaa
    29641 cttaaattaa gattaatttt tacgcaattg aatattgcaa acacgaaggg gttaaccaca
    29701 aatagatatc ctaagtgtta gtaacccgga cttaatccat tggagaatgt tttttat
//
LOCUS       DBNCWK010000024        27924 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.024, whole genome shotgun sequence.
ACCESSION   DBNCWK010000024 DBNCWK010000000
VERSION     DBNCWK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 27924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27924
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.024"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(39..1244)
                     /gene="tyrS"
                     /locus_tag="AC06IO_000981"
     CDS             complement(39..1244)
                     /gene="tyrS"
                     /locus_tag="AC06IO_000981"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIO9874189.1"
                     /translation="MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTA
                     PDLHLGHTVLLNKLRQFQQFGHEVIFLIGDFTAMIGDPTGKNVTRMPLSQETVLENAK
                     TYQHQVFKILDPDKTTVAFNSQWLNKFNAVDLIRLAATHTVARMLERDDFNKRYTTGQ
                     PIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLI
                     EGLDGVKKMSKSLDNYIGINETPEQMFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQ
                     SVLEGKNPRDVKIDFAKEIVARFHDQTQAEFAHNKFIERFQKGNIPEDLEELSLVIAE
                     PIALAQLLKQIDLTASTSESIRMVKQGAVKVDGDKISDPSLKLPIGKSYIIQVGKRRI
                     AKLSIQQAH"
     gene            1501..2934
                     /locus_tag="AC06IO_000982"
     CDS             1501..2934
                     /locus_tag="AC06IO_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIO9874190.1"
                     /translation="MDKRPNIPKRKSGNSSKLLAFFALVFAFSLPYLLVKNFSHPKRS
                     SYADQTDLYPELSDELLRDIPAEASTEIPHEKPDIKSATKPMQTKQDQQDKPINTSHV
                     KKDNEWQIVNPHSGDSMATIFHRLGLTAQNLNDVIKNNPHAKMLTRIKPNQKLQFLIK
                     KNKLERLIVPVNTIQTLTVYRAGKIYKTKIDSKKTTTQNRYVTATVQGSLYTTAQRFN
                     IPSKLVRQMTTIFNKEIDFSRSLRSGDQFSIIYDAYYIEDKMVGVGDILVVTYTNRGK
                     TYQAIRHTNAHGEQDYFTPQGTSFKKAFTRYPIKFSHISSTFALSRYHPILHYRRAHK
                     GIDLAAPIGTPIHATGDGIITIIDRHNGYGNMIKIKHDKTYSTVYGHLLKFQKGLSKG
                     SRVKRGQVIGYVGQTGLATGPHCHYELHVNNQPRNPTTISLPTAAPVPAREMAAFKAK
                     ASTLLARLKLFEQAQLASKGKKKASVG"
     gene            complement(3065..3598)
                     /locus_tag="AC06IO_000983"
     CDS             complement(3065..3598)
                     /locus_tag="AC06IO_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HIO9874191.1"
                     /translation="MNGFANHLSNWLFLTFIVGIPLYAAIKKVNVFDAFIIGAKQGFD
                     VSVKLIPYLVAMMVAIGMLRASGFFSLMSQILSPALAMIGMPSELLPLALIRPFSGSA
                     STGVMAELIHKYGGDSFIAKTAATMMGSTETTFYVIAVYFGSVGIRRTRHAIPAGLLA
                     DLAGIIASVLICRYLFT"
     gene            complement(3595..4200)
                     /locus_tag="AC06IO_000984"
     CDS             complement(3595..4200)
                     /locus_tag="AC06IO_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIO9874192.1"
                     /translation="MLNLIWLGMILISVVVGVIEGRIDEVVRAVTDSAKLGFELALGL
                     AGIMTFWLGIMSVATESGLVSILGKVLKPVLKRLFPDIPVEHPAMGAMVMNIAANMLG
                     LANAATPFGLQAMKELQSLNQNLKVATNSMCTFLAINTSSVQLIPVTAIAFLAANGST
                     NPSSVVFTSLIATTVSTLVAIVAVKQLAKLPRFKIERTDSL"
     gene            4270..5340
                     /locus_tag="AC06IO_000985"
     CDS             4270..5340
                     /locus_tag="AC06IO_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HIO9874193.1"
                     /translation="MKHTYNPFRYLIYFAFFITSLSSYSSQTTNIKEQTDTGLAPPAP
                     LITTNSAAIEEQANSSIRKLYHTLNTMPNTSMADRISQISAYFKGTKYILGSLGEGPN
                     ARYDQFPRYRVDGFDCDTYVNTVLSLALANSLESFQECLKHTRYKNGRRSYINRNHFT
                     SIDWNNYNQKRGLLKDITFSIRNEKKQPVALYANALINKPQWYNHKTVDTIRLQKQDK
                     NEQEKRLVELKAKGKTLETSLSNVPYIPFTALFSENKPNLHLFSQIPNGAVIEIIRPN
                     WDLRQQIGTELDISHLGFAIWINNELFFRQASSQYGKVVDVSLIDYLDKARSSPTIKG
                     INIQVVLPEKPVSNGCQLFDIR"
     gene            complement(5584..5877)
                     /locus_tag="AC06IO_000986"
     CDS             complement(5584..5877)
                     /locus_tag="AC06IO_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIO9874194.1"
                     /translation="MTQYNFERWSEMAKKIQEPFQAIAELNVKTLQGLSYLKPDELAQ
                     VKKPEEMLEKQINLAVENGHKALDYMQKSFQIIEKAMLNLVQEAKKTTAEVKK"
     gene            complement(6084..6482)
                     /locus_tag="AC06IO_000987"
     CDS             complement(6084..6482)
                     /locus_tag="AC06IO_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HIO9874195.1"
                     /translation="MTRLIKKYKNRRLYDTEISQYITVEQLQKYVVDGIDFKIEDSET
                     GKDLTNSVLLQIIVEMEAGPTQFLSSEILRQIISLANHPMHVSFKEMMEQMFHAMDKP
                     LQNNPYQQATEVWNKQMQQMVHQWQKMFKV"
     gene            complement(6612..7358)
                     /gene="phbB"
                     /locus_tag="AC06IO_000988"
     CDS             complement(6612..7358)
                     /gene="phbB"
                     /locus_tag="AC06IO_000988"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIO9874196.1"
                     /translation="MLNNNVVLITGGTGDIGTAVAKQLDSTFKHVIALDLVSYDEGEA
                     WLHDLKTQGYKNINFRHMDVTDYEQCAEVIGLIIEEFGGIDVLINNAGITRDAVFTKM
                     TKRQWDEVLRVNLDGMFNVTKQVVESMKAQESGRIVNVSSVNAQKGQFSQANYAASKA
                     GVYGFTKSLAQELMIKNITVNSISPGYVNTRLMKGIRPDILEGIINLIPAKRLAEPQE
                     IAWAIEFLISEKSRYITGANLSVNGGLHMY"
     gene            complement(7536..8276)
                     /gene="phbB"
                     /locus_tag="AC06IO_000989"
     CDS             complement(7536..8276)
                     /gene="phbB"
                     /locus_tag="AC06IO_000989"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIO9874197.1"
                     /translation="MDKMIAIVTGGTGGIGSAISQRLADSYQVVACYYKDGRHEEAKK
                     WQDEQKQLGYDIDIVYGDIAQYSDCEKITSLVMERYGRIDVLVNNAGITKDCSLRKMT
                     PQQWQQVLDANLTSVFNMTRNVVPVMLERGYGRIISISSINGRKGQFGQCNYASTKAA
                     LFGFTKSLALEVASKGITVNTVSPGYIETPMLAALKEDVLNSIISSIPVGRLGYPKEI
                     ADAVAFLASPDSGFITGANLDVNGGQYM"
     gene            8542..8940
                     /locus_tag="AC06IO_000990"
     CDS             8542..8940
                     /locus_tag="AC06IO_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874198.1"
                     /translation="MFNKFLVTTLSIVTLTLSPYSFAKDDSCEVQVAVSTPPVQFDQS
                     VAFNVTNDDGISKSIILQGGSSPKTIGKLICSPLPYTISATQYFTPTGLTNKHAQPVG
                     QCVLKAGNVILNGSNNSVAVVFPNDFICHH"
     gene            complement(8982..10169)
                     /locus_tag="AC06IO_000991"
     CDS             complement(8982..10169)
                     /locus_tag="AC06IO_000991"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIO9874199.1"
                     /translation="MVFGFLNLSFWGYVLATFILTQITIASVTIYLHRNQTHRALTLH
                     PVVSHFFRFWLWLTTGMVTADWVAIHRKHHATTDVEGDPHSPVVEGIRKVFWQGAELY
                     REARKNRDMVAKYSHGTPTDWIERNVYSRHSSKGILLMLLLDLFFFGIPGMTVWAIQM
                     MWIPLFAAGVINGIGHYWGYRNFECPDAATNIIPWGFWIGGEELHNNHHTFASSAKFS
                     VKWWEFDIGWMYIRILSFFGLAKVKKLPPKLAMDEGKFHVDLDTVRAVISNRFQVMSD
                     YYKNVIRPILKYEKNTGIETKEDKKLFQRAGRLLRREDKLLSTKAKTRLQKLLDAREQ
                     LRVVYSYKQSLQNVWLKTATTQKELVEALQQWCRQAEESGLDVLKQFAQQLKGYVPVY
                     TRE"
     gene            10350..10580
                     /locus_tag="AC06IO_000992"
     CDS             10350..10580
                     /locus_tag="AC06IO_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874200.1"
                     /translation="MSDKVQKLIENIVPFIVIGVGIAIVIALLFMFFYVAIWGVIIGG
                     ILWLGALAKEYFFPSKSSQKEQGRIIEHDDKK"
     gene            10580..11404
                     /gene="mutM"
                     /locus_tag="AC06IO_000993"
     CDS             10580..11404
                     /gene="mutM"
                     /locus_tag="AC06IO_000993"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIO9874201.1"
                     /translation="MPELPEVETTKQGIKPHLEGRMITAVQVRNRKLRLPVPLNLNEL
                     CEGKHITAITRRGKYILLHMDKGYLLIHLGMSGHLRIVSQTANPQKHDHVDLHINNGL
                     ALRFCDPRRFGLFIYIDENPYQHPLLAHLGPEPLSDDFNSEYLLRKAANKSQSIKSFI
                     MDSQIVVGIGNIYAAESLFLAKIHPNTSAKKITTEEFNSLTGHIKKILESAIEAGGTT
                     LRDFYSSDGKPGYFRFALKVYGRKNLPCLVCENKIETVVIAGRHSAFCPHCQPIIT"
     gene            11479..12099
                     /locus_tag="AC06IO_000994"
     CDS             11479..12099
                     /locus_tag="AC06IO_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874202.1"
                     /translation="MDIKKLKKAASNFLERYPGGFYHPEIIEMGKKHKMDKMVALAQG
                     SFAKKNFKNPDEITNSMLKIVTQSSLISIFEKPKFRDFIKRTPPNDKVLIAKGLKELL
                     YGNEQEGFEELLFQFQREKLAKWTLMTIFQAYFNPEVDVFIKPTTTKNIIKKLNLDIV
                     YNPTPSWEFYESYRNIINQMKTKIEKSLAPSNAAFCGFLMMSLDNR"
     gene            complement(12157..12227)
                     /locus_tag="AC06IO_000995"
     tRNA            complement(12157..12227)
                     /locus_tag="AC06IO_000995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12193..12195),aa:Gly,seq:ccc)
     gene            12539..13606
                     /gene="dinB"
                     /locus_tag="AC06IO_000996"
     CDS             12539..13606
                     /gene="dinB"
                     /locus_tag="AC06IO_000996"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIO9874203.1"
                     /translation="MNPIRKIIHIDMDCFYAAIEMRDFPELANKPIAVGGNAKRRGVI
                     ATCNYAARQFGIRSAMPTAHALKLCRELILRPVRMDVYQKESQYIRSLLTEYTDLIEP
                     LSLDEAYLDVTESTQCQGSATWIAEEIRARIYQTRQLTASAGIAPNKSLAKIASDWHK
                     PNGQMVIRPEDVSAFVLDLPVRKLFGVGPKMEEKLGALNIKTCADLQRYSIEYLLQKF
                     GTMGQRLYELARGIDNRPVNPERIRKSISVEETYPKDLPNSEACLAVLPDLMARLEAR
                     IQRAGKISGIHNLFVKLKFNDFQQTTIERVMDKIDLIVLRQLIQEGFARRGMPVRLLG
                     IGIKLKQENTYQSVQLPLLDL"
     gene            13850..14731
                     /locus_tag="AC06IO_000997"
     CDS             13850..14731
                     /locus_tag="AC06IO_000997"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIO9874204.1"
                     /translation="MQPFIIDEKLVCRLIASQFPHYSNLPVKPVAVGGWDNRTFHLGK
                     DMLVRLPSAEQYELQVEKEQQWLPQLASLLPVPIPTPLAKGMPGEGYPWKWSIYRWLE
                     GETIVSANLSNLNEIAKDLATFLTAFHQIDSSGGPKPGMHSFYRGGDLKIYDPETRQA
                     IDYLKGKIDTDHATEIWETALNTSWQGTPVWVHGDISAGNLLVKNGKLCAVIDFGQLS
                     VGDPACDLAITWTLFAGDSRKIFREMLALDKGTWFRGQAWALWKALIVAAGIVNTNAM
                     EVQKSCRIINELFLTEL"
     regulatory      14786..14849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            14873..15193
                     /locus_tag="AC06IO_000998"
     CDS             14873..15193
                     /locus_tag="AC06IO_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIO9874205.1"
                     /translation="MLLPWLYLIIAGLLEICWAVGLKYTDGFSKWQPSLFTVTTLIIS
                     MFLLAKAAQTLPIGTAYAVWVGIGSLGTAIMGILLFQEHLSVFRLLYLVLMLISIFGL
                     KYTA"
     gene            15262..15993
                     /locus_tag="AC06IO_000999"
     CDS             15262..15993
                     /locus_tag="AC06IO_000999"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIO9874206.1"
                     /translation="MKISRLKTFWIITRLFYYTGVACTRSIFKYLFSTPTRPWVDKVI
                     HHWVDQLLNAVKVEYKVINPHNVQPQPGKATILMCNHSSAYDIPLGFKAFPNNSIRML
                     AKRELSKIPLLGKGMAAAEFPFIDRKNRFQAIKDLEFAQQLMESGIILWIAPEGTRSK
                     DGKLAPFKKGGFITAIQAKATIIPIGIRGAFDILPARTFNIHLNQKAEIHIGEPIDAA
                     QYTIENKEELIKKTHQSIKKLVEEN"
     gene            16203..18419
                     /locus_tag="AC06IO_001000"
     CDS             16203..18419
                     /locus_tag="AC06IO_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9874207.1"
                     /translation="MSINKIEEDRDTSSDLEMKAKMYLVHRMDKAGPLTVKDVHILIN
                     KLSKDLQKNQLTLIDCQLIINLVESRFTLAKQATRYLQFFLNKLDGKSINPILIPLSN
                     KPFWQTEPHPFEHYRSTDQLPEKADIVIIGAGLTGASTAYHLIDAVKKRNLTVVLLDK
                     GNPATEASGRNGGNFELLPENSVGIYSGLVSERFRFLKRCYPHVHPSILQIESEYHAS
                     LVFQFALKNRNRFKEIVRKERIYCDLAAKGWLYIAHTEEEEQAICDEVTLAAQHGERI
                     YIWSRKKIREQFGINSEFIGRFIPDDGTYNPFKYTCCLIKAAIKSGIKLYTFVKVQQI
                     KSMDTEYHQLKTNMGLLKARQVIVATNAFTSELLPELSAIKPHQSQIAITDSTPDYCK
                     GRLITSEDGPLYLNQPRVRSKNGLVPLLIGAGDDRPMKNPYYRKRSKKVHDLIVQQRD
                     KYFPNLKGKPFSTEWVGALAFTPDQLPAIGYLSPGIIIVMCCNGYGGSYTTAAGLTAA
                     EIALTGKNPPWLPAEVFSPKRLLSDKPLFWEDSDSLWRIGKTLCTTLNNLNQLLAECL
                     SYQSMYQPYSTISSLPHGHQEKTCNLDPLRVMHQLAIFRTFAYQELEMLIRYTSPVCL
                     SKGEILFKQGDAGHSCFILIQGKINLYYEHTEGFTSMVAELEAGSVFGQMALFLENKR
                     MATCQATENCSMLEIMVEPCKSLFIQQQALGIKLLRLLNQGVIDALHKLNKRLKYT"
     gene            complement(18517..20241)
                     /gene="ggt"
                     /locus_tag="AC06IO_001001"
     CDS             complement(18517..20241)
                     /gene="ggt"
                     /locus_tag="AC06IO_001001"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIO9874208.1"
                     /translation="MKDHYKWLSCLFFLICNIYQVNAKTFPQHPPGYAVASAHPLATH
                     AGLEILAAGGNAFDAAVAVAAALAVVEPYHSGLGGGGFWLLHQEKEHLNLFIDGREVA
                     PLAASKDMYLAPDGQVIPGLSLNGGLSAAIPGQPAALVYIAKNYGRLPLSKTLAPAIK
                     LAEEGFPVDPQFRYFSTLDDRLENLKKYPDTAAIFLKNGQPFEIGDKLVQKDLAKTLR
                     LIAKYGNAGFYSGETASLLVQGVNAAGGIWSLDDLAQYKIKVRPPLISAYHNMLIITA
                     PLPSAGGVALVTMLNILSKFPLSSFPKLKWVHYVVESMRLAYWQRDQFLGDPDFIKVP
                     LDKLISSDNAKQLSRLISPNKAIDSKVLQGSVPTGKHSNTSHISIIDTEGNRVAATMT
                     INYIFGSSVVAKGTGVLLNDEMDDFSTKVGEQNVFGLVGSERNAIEPRKRPLSSMAPT
                     FLEMPGRVAILGTPGGSRIPTMVLLASLLFYDGYGAISMVSAMRFHHQYLPDVLQFEP
                     DTFPSSIQQGLQAMGYHLMPLKQYYGDMQAITWDKESNVLTAASDPRHIGLAAAISNM
                     AYGYGVNY"
     gene            complement(20225..20737)
                     /gene="coaD"
                     /locus_tag="AC06IO_001002"
     CDS             complement(20225..20737)
                     /gene="coaD"
                     /locus_tag="AC06IO_001002"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIO9874209.1"
                     /translation="MQMVINEMKQKAIYPGTFDPVTNGHIDIITRASTIFPELIVAVA
                     SNKNKRPYLSWESRISLLEESVGHLTGVRVVGFDNLLIDFVLEQNAGIILRGLRAVSD
                     FEYEFQLAGMNRKLSKKVETLFLTPAEHLMYISSTLVREIAALNGDISQFVPPNVVRE
                     LKKRQNERSL"
     gene            20800..21399
                     /gene="lolB"
                     /locus_tag="AC06IO_001003"
     CDS             20800..21399
                     /gene="lolB"
                     /locus_tag="AC06IO_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIO9874210.1"
                     /translation="MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETAT
                     VSSWEIKGALAAKTKAKGWSATMNWVQQGANSYQIRLMGPLGGGAVLISKKGNTITFQ
                     DGSKITTSTNADELLLKQTGIRLPVNNLYYWVRGLPAPGGVQSEKHDQFNHLIQLKQN
                     GYTIDFNKYTSVKGIDLPSMIRLEGNGVMVKVVIKNWVV"
     gene            21527..21601
                     /locus_tag="AC06IO_001004"
     tRNA            21527..21601
                     /locus_tag="AC06IO_001004"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21559..21561,aa:Gln,seq:ttg)
     gene            21781..22728
                     /locus_tag="AC06IO_001005"
     CDS             21781..22728
                     /locus_tag="AC06IO_001005"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIO9874211.1"
                     /translation="MSTMMIFTGNANPELALKISSHLQIPIGKATVGTFSDGETMVEI
                     LENVRGKDVFVLQSTCAPANNNLMELLIMADALRRSSAGRITAVVPYFGYARQDRRVR
                     SARVPITAKVVADMMASVGICRVLTVDLHADQIQGFFYMPVDNVYSTPVLLEDITKQK
                     LNNIMIVSPDVGGVVRARAVAKRLNDAELSIIDKRRSGPNKSEVMHIIGEPANKNCII
                     VDDIVDTAGTLCTAAHELKKNGAKSVRAYITHPVLSGPAVNNIKHSGLDEVVVTDTIP
                     LSAEAQNCEKIRVVSLADMLAQAIKRVNVEESVSSMFAE"
     gene            complement(22790..23071)
                     /locus_tag="AC06IO_001006"
     CDS             complement(22790..23071)
                     /locus_tag="AC06IO_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9874212.1"
                     /translation="MTTISNDAGDTTASLAESVTQSVQKYFSELKGTDPVDLYQFVLE
                     EIETPLFRAVMEHCKYNQSRAAIMLGISRGTLRTKLRRYFDDKYVGTRD"
     gene            complement(23261..24718)
                     /locus_tag="AC06IO_001007"
     CDS             complement(23261..24718)
                     /locus_tag="AC06IO_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HIO9874213.1"
                     /translation="MKKLTGLVIILAVLILGGYYGMGILTERTVKKTVEVINQSNGLF
                     ADIQQYKRGWFCSDALVKWRLHVPERVVKDDDGKLQTTPAQDYQMEMPIKIYHGPIIY
                     ANKQLRFGMGFAQTVFPFPEKYNQQFNELFSQDSEKPQLDLSIFVNYLNKSTVELSIP
                     SFKLTAKDGNGKFNWMGMDSTTSMSSNLDKVEGEIEFDGAEFSKDDTKLSLGKVTSEY
                     DLHKTTTGLYLGQASFSLPSLAVVVKDQKMFEISELTLSSSSDIDDNLFSTHFSAALK
                     SLFANGQNYGPGEVEMSLRNLDAEVLAKINEQANAMQRGTDQERQQALMAMLPELPKL
                     FSKGAEFEISKLSLKLPQGMIDGNLLITLPKGDSSNPFELIQKTQGNAKLKMPIEVVK
                     QLMQQSIMQQMAKQPEMQQALIQQLQNSQGQSNQAEPTPEQIAAMQVDKQINELEQSG
                     LITVDGKDYVIEMSLSEGKFTVNGKPFDPSMMKFQ"
     gene            complement(24948..26231)
                     /locus_tag="AC06IO_001008"
     CDS             complement(24948..26231)
                     /locus_tag="AC06IO_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874214.1"
                     /translation="MGISKVEIYKRKNYLGFAWLVWGLAAAFYFSDYLARVAPGVMHR
                     YLQMDFGINEAGFGILTASFYVPYILMQIPVGLTVDRLSIRWLLTIMSLVTAFGCCVF
                     GLADGLLTASIGRMLIGFSAAFAFICSLRLATSWFPPTMLGLLSGLTQALGMLGAAAG
                     EAPVSFLVSNVGWRHSMLIIAFLFIALSGLLYQFVQDKPGEHRNEIRSVNRISILDSL
                     KIILSNKQTWLNAMYAGFLFGPTAVIGEAIGPAYLQFGRGLGAHAAAFATGLIFIGWG
                     ISGPLSGWISDKMGRRKPLMIISAVCGVILSSLFVFIPEMSQTTAYILFFVFGLTNTG
                     VAISYAVSTEIHDRSVVGTSIAFTNMTSIFVGALFQPLVGRIIDMVSGPRAYNVETLL
                     LSDFQAGLKLLPLCSLVALILAFTVKETYCKPIRH"
     gene            26881..27270
                     /locus_tag="AC06IO_001009"
     CDS             26881..27270
                     /locus_tag="AC06IO_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIO9874215.1"
                     /translation="MNKLNIAIKEAFESSGSSKEANKVYLEFIKANFIIPIESNSDDS
                     EPIVLFLEENNHVFLPVFTDMKYLDAWASEISQHIKLLKLSGVDLLKGLGEKVTVCLD
                     IGSELYKEFNPSEIERMRSMVLKLFKN"
     gene            complement(27341..27712)
                     /locus_tag="AC06IO_001010"
     CDS             complement(27341..27712)
                     /locus_tag="AC06IO_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmission trait enhancer LetE"
                     /protein_id="HIO9874216.1"
                     /translation="MNDTTSLLSHLRLRFNIEHPSYEECYIFGYECALAEVPEEDNPF
                     REGSQEAEQWLEGWWAGIYGEKPLFDLNNIEEDILLDKTTAANDQQYHHKNSFLSLVF
                     EIGGAIAASAIVGYQLLELVA"
BASE COUNT         8977 a         5655 c         4902 g         8390 t
ORIGIN      
        1 ctgtcaaata cttttttaat tttttttaaa aaattatctc agtgtgcttg ttgaatgctc
       61 agcttggcaa ttcgacgttt tccaacttga ataatataag acttgccaat aggtaatttt
      121 agggaaggat ctgaaatttt atccccatcc actttaacag ccccttgttt caccattctt
      181 atagactctg atgtactggc tgtcaaatca atttgcttta ataattgtgc taaagctatc
      241 ggctctgcaa taactaacga caattcttcc aagtcttcag gaatatttcc tttttgaaat
      301 cgttcaataa acttgttatg agcgaattcg gcctgagttt gatcatgaaa tcgcgcaaca
      361 atttctttgg caaaatcaat cttaacatct ctgggatttt tcccttctaa tacagattgt
      421 tttaattgtt gtatttcctt gcctgtttta aagcttaata aatcaatata tcgccacatt
      481 aactcatcag aaactgacat gatttttccg aacatttgct caggtgtttc attgatccca
      541 atgtaattat caagggattt cgacattttc tttacaccat ccaaaccctc tattaatggt
      601 gtcatcatga caacttgagg ctcaaagcca taatgcttct gtaattctct acccatcaat
      661 aaattaaatt tctgatcagt gccgccaagc tcaacgtcag cttttaatgc cactgaatca
      721 taaccttgta acagcgggta caaaaattcg tgaattgcga taggctgtcc tgtagtataa
      781 cgtttattga aatcatctcg ttccagcatt cttgccacag tatgtgttgc agccagtcga
      841 atcaaatcaa ccgcattaaa tttattaagc cattgtgaat tgaaagccac agtagtctta
      901 tcagggtcga gaattttaaa tacctggtgt tggtaggttt ttgcattttc cagaaccgtt
      961 tcctgactca atggcattcg ggttacattc tttccagttg ggtcaccaat catggctgtg
     1021 aaatcaccaa tcaaaaaaat aacctcatga ccaaattgct gaaactgtct caatttatta
     1081 agcagtacag tatgtcccaa atgaagatct ggcgctgttg gatcaaaacc tgcctttatt
     1141 ttcaatggga tacctttttg taatttcttc tccaactcag gtacaggaag aatttcctca
     1201 cacccccgca ttaattcgga acaaactgaa tcttgaacta tcatgtcatt aaaacccgta
     1261 taaatgattg acctatcttc tcgcagcgag tatcttagcc cggttagcaa tttcctgcta
     1321 taccaaatga tacattttga gataatttat tactctaaat tgagaaaaat aaaacaaaat
     1381 atccaaataa gagtcgattt agttgactca aggtagcggt cacttccaaa taatgactgt
     1441 gatatattga ttgaaattta aatagcagca tttttcagat gacggcaata taggcaaaaa
     1501 atggataaaa gaccgaatat accaaaaaga aaatctggta actcatcaaa gctattggcg
     1561 ttttttgcgt tggtatttgc cttttcgctt ccgtatttgc tagtgaaaaa tttttcacac
     1621 cctaaacgaa gcagttatgc tgaccaaact gatttatacc ccgaactatc agacgagtta
     1681 ttaagagata tacctgctga agcttccacg gaaattccgc atgaaaaacc tgatattaaa
     1741 tctgctacaa aacctatgca aacaaagcaa gatcagcaag acaaaccaat caatacaagt
     1801 catgtaaaaa aagataatga atggcaaata gtcaatcctc attcaggaga ttctatggcg
     1861 accattttcc ataggctagg actaaccgcg caaaatttaa atgatgtgat aaaaaataac
     1921 ccgcacgcta agatgcttac aagaattaaa cccaaccaga aattacaatt tttaattaaa
     1981 aaaaataaat tggaaagatt aattgtccct gtaaacacca ttcaaacact aacagtttat
     2041 cgcgcaggaa aaatatacaa aaccaaaatt gactcaaaaa aaacaaccac tcaaaatcgt
     2101 tatgtgactg caacagtcca aggttctttg tatacaacag cccaacgatt taacatcccc
     2161 tcaaaattag tccgtcaaat gaccactatc tttaacaaag aaattgattt ttccagatct
     2221 ttgcgctccg gagatcaatt ctcgataatt tatgatgctt attacattga agataaaatg
     2281 gttggagtag gagacattct ggtagtaacg tatactaatc gtggaaaaac ttatcaagca
     2341 atacgtcata ctaacgctca tggtgagcaa gactatttta ccccacaagg caccagcttt
     2401 aaaaaagcct tcactcggta tccaattaaa tttagccata taagctcgac ttttgcttta
     2461 tcgagatacc acccaattct tcattacaga cgagcgcaca aaggtatcga tttggctgcc
     2521 ccgattggaa caccgataca tgctaccggc gatgggatta ttacaatcat cgataggcat
     2581 aatggatatg gcaatatgat taaaattaag catgataaaa catacagcac tgtttatggg
     2641 catttactca aattccagaa aggtctatca aaaggcagta gagtaaaacg aggtcaagta
     2701 attggttatg taggacaaac cggtctggca accggacctc attgccatta tgagcttcat
     2761 gtcaataatc agccaagaaa cccaactaca ataagcttgc ctactgcagc accagtacca
     2821 gcaagagaaa tggcggcatt caaagcaaaa gccagtacac ttttagcccg gctaaaactt
     2881 ttcgagcaag cacaattagc aagtaaaggg aaaaagaaag ctagcgttgg ataaagactg
     2941 cgcgtgggat ttttccatac ccaaatacct aatcaaaatg acagcagaca cttctttata
     3001 aagcgactaa gctccaagta attcaaacat aacgttactc tttttgtgtt aaaaaaatct
     3061 tatatcatgt aaataaatat cgacaaatca aaaccgaggc aataattcca gctaaatctg
     3121 ccaataatcc agcaggaata gcatgtcttg ttcttctgat acccacagag ccaaaataaa
     3181 cggcaatcac ataaaaagta gtctctgtac ttcccatcat tgttgctgcg gtttttgcga
     3241 tgaatgaatc ccctccgtat ttatgaatca gttctgccat aactcctgtt gaagcacttc
     3301 cagagaatgg tctgataaga gctaaaggta ataattcgga aggcattcct atcatagcca
     3361 acgctggcga taagatctga ctcattaagg aaaaaaaccc cgaagctctt aacatgccaa
     3421 tagcaaccat catcgcaact agataaggta taagctttac gctcacatca aaaccttgtt
     3481 ttgctccaat tatgaaagca tcaaatacat ttactttttt aattgcagcg tacaaaggga
     3541 taccaacgat gaaagtcaaa aacaaccaat tggataaatg attagcgaat ccattcataa
     3601 gctgtctgtc ctttcaattt taaatcgcgg taatttagcc aattgtttta cagcaacgat
     3661 agctaccaga gtagatacag tagtcgcgat caaagaagtg aagaccacac tacttgggtt
     3721 tgtgctcccg tttgcagcaa gaaatgcgat agcagtgacc ggaattaatt gcacactgga
     3781 agtgtttatt gccaagaaag tacacatgga attcgtggca actttcaaat tctgattaag
     3841 gctctgcaat tctttcattg cctgcaatcc aaaaggggtc gcggcattag caagacccaa
     3901 catgttcgca gcaatattca ttaccattgc tcccattgca ggatgctcaa caggtatatc
     3961 aggaaataat cgctttaaca ctggtttgag tactttacct aaaatagaaa ctaaacccga
     4021 ttctgtagca actgacatta ttcctaacca gaaagtcatt atcccagcca aaccaagcgc
     4081 taactcaaat cccagtttgg cagagtcagt aactgctctt accacctcgt caatacgccc
     4141 ctcaataact ccaaccacca ctgaaatcag tatcattcct aaccatatta gattgagcat
     4201 tattgcctcc aacctctgaa aaaggttaat atgtaaccaa aattagattt aaaataccaa
     4261 tcaggactta tgaaacatac ttacaatccc ttcagatact taatatattt cgcttttttt
     4321 atcaccagcc tttccagtta tagcagccaa acaaccaaca ttaaggaaca aactgataca
     4381 ggtttagccc cacccgcccc tttgattacc actaactcag ctgctattga agaacaggct
     4441 aattcttcaa ttaggaaact atatcacaca ctcaatacca tgccgaacac ttctatggca
     4501 gatagaatta gccagatcag cgcatatttt aaagggacaa aatatattct tggctcttta
     4561 ggtgaaggtc cgaatgctcg ttatgatcaa tttcccagat atcgagttga tggctttgat
     4621 tgtgatacct atgtcaatac agtactttct ctagcattag ctaactctct ggaatcattc
     4681 caggagtgtt taaaacatac tcgctataaa aatggaagaa gatcttatat caacagaaat
     4741 cattttacct ctatagactg gaataactac aatcagaaga gaggtttact taaagacatt
     4801 accttctcta tcagaaatga aaaaaagcag cctgttgctc tttatgctaa tgccttaatc
     4861 aacaaaccac aatggtataa tcataaaacg gtcgatacca ttcgattgca aaaacaagat
     4921 aaaaatgagc aggaaaaacg tcttgtggaa ctaaaggcca aaggtaaaac gctggaaact
     4981 tctctttcta atgtccctta tattccgttc actgccttat tttcagagaa caaacctaac
     5041 ctgcatttat tttcccaaat tcctaacggg gctgttatcg aaattatcag acctaattgg
     5101 gacttacgcc aacaaatagg gacagaatta gatatttcgc atttaggctt tgctatttgg
     5161 attaataacg aactgttttt tcgacaagcc tcttcccaat atggtaaggt agttgatgta
     5221 tccttaattg attaccttga taaagcacgc agtagcccaa ctataaaagg gattaatatt
     5281 caagttgtat tgcctgaaaa accagtatcc aatggatgcc agctttttga tattcgatag
     5341 cttataaatt aatatcttaa tcgttaacct ataattttgc gtaattacgc acgtataacc
     5401 attatttatt ttatgaaatg tcgggagttt ttattaacga gatactcgtg tttcttagta
     5461 aaaaatcgat ttctatttta attgactcgc ttggctatta tctagcagtt ggcgactgga
     5521 aatatgctca atgatatgaa aatagaaaag gcttattttg atcaaataag ccttttaaag
     5581 gaattatttc tttacttcag cagttgtttt tttagcttct tgcaccaaat ttaacatggc
     5641 tttttcaata atttgaaatg atttttgcat ataatccaaa gccttgtggc cattttccac
     5701 tgccaaatta atttgttttt ccagcatttc ttcgggcttt ttaacttgag ccagttcatc
     5761 aggcttcaaa taacttaacc cttgcagggt tttaacattc agttcagcaa tggcttggaa
     5821 aggctcttgt attttttttg ccatttcact ccatctttca aaattgtatt gggtcataat
     5881 attctccatt tgttgcactg cacaatataa tagtagacct tatctttatt atttgtcaat
     5941 atcttatcta attatttttt gcattgcacc aaaaatcacg attacattca tggatttttg
     6001 atgtgtattg cactttccaa atcatcagta aaattcgtaa agcgaaacgt tctattaact
     6061 gcaaaaaagt ttgggaggaa tttttagact ttaaacattt tttgccattg atgcaccatt
     6121 tgttgcattt gcttattcca gacttcagtc gcttgctggt aaggattgtt ttgtaaaggt
     6181 ttgtccattg catgaaacat ttgctccatc atttccttaa aggaaacatg cattggatga
     6241 ttagctaatg aaatgatttg acgtaaaatt tctgaagaca aaaattgcgt tggtcccgcc
     6301 tccatttcca ctataatttg caacagaacc gaattagtta aatcctttcc tgtctctgaa
     6361 tcctctatct taaaatcaat accatccaca acatactttt gtagttgctc aacagtaata
     6421 tattgactaa tttcagtatc atacagtctt cgatttttgt atttttttat gagtcgagtc
     6481 attattttct caagtatcag atatagaaat ataattataa aatgatgtgc aaaattggta
     6541 tagtttgctt ctttaagccc aagagtaaat ctttcacact cctttcacaa attctttcaa
     6601 ggttaaaaaa gttaatacat atgcaaacca ccattgacac taagatttgc tcccgtgata
     6661 tatcgacttt tctcactaat cagaaattca atagcccaag caatttcttg tggctctgcc
     6721 aatctttttg cgggtatcaa attaataatc ccctccagaa tatctggtct aattcctttc
     6781 atcagacgag tattaacgta accaggggaa atactgttta ctgtaatatt tttaatcatt
     6841 agttcctggg caagactttt ggtgaaacca tatactccag cctttgaagc agcataattg
     6901 gcttgggaaa attgcccttt ctgggcatta accgaagaca cattcactat tcggcctgac
     6961 tcctgagctt tcatactctc tactacttgt ttagttacat taaacattcc atcaagattg
     7021 acacgcaaca cttcatccca ttgccttttg gtcattttgg taaacacggc atctcgagta
     7081 attccagcat tattaatcag cacatcaata ccgccaaact cttcaataat taagcctatt
     7141 acttcagcac attgttcata atcagttaca tccatatgcc tgaaattaat atttttatag
     7201 ccttgtgtct tcaaatcatg aagccatgct tcaccctcat cataggaaac caaatccaaa
     7261 gcaatgacat gtttaaaagt tgaatccagt tgttttgcaa cagccgtacc aatgtcacca
     7321 gttccaccag taattaatac cacattatta ttcagcatga atttctctat tagttattcc
     7381 tatccccaat tgatgattta aaattggagc ttatatttta ttcattgtta acggtgatcg
     7441 cagccacttt ttcaaaatcc caaggtgcct tgcgaacagt aaataatatg ttcacaaaac
     7501 ctcttgagat tcttaatcgg tgaatcaaac tctgactaca tatactgacc accattgacg
     7561 tccagatttg cccccgtaat aaatccgcta tctggtgatg ctaaaaatgc aacggcatct
     7621 gcaatttctt ttgggtatcc caatcgacca acaggaatac tggatatgat tgaattgagt
     7681 acatcttctt tcaatgcagc gagcatagga gtctcaatat acccaggcga cactgtgttt
     7741 accgtaatac ctttacttgc tacttctaat gcaagactct tggtaaaacc aaataaagcc
     7801 gccttggtgg aagcataatt gcattggcca aactgccctt ttcttccatt gattgaagat
     7861 atactgatga tacggccata acctctttcc aacatcactg gaacgacatt gcgcgtcata
     7921 ttgaatacac tggtcaagtt ggcgtctaaa acctgttgcc attgttgagg tgtcattttt
     7981 cttaaactgc agtctttagt aatgcctgcg ttgttaacca aaacatctat acggccataa
     8041 cgctccatca ccagggatgt gattttttcg caatcactat attgagctat atctccatat
     8101 acgatatcaa tgtcataacc aagttgcttt tgctcatctt gccatttttt tgcttcttca
     8161 tgtcttccat ctttatagta gcatgccaca acttggtaac tatcagctaa gcgctgacta
     8221 atagcagaac caataccgcc tgttccccca gttacaatcg ctatcatttt atccatcgac
     8281 aactcctttt gttgctaggt aacattacat tactacaatc taattttact ttcttgtcaa
     8341 tgacatatat ataatactaa gttggtgata ataattcagc tactttattt ttgatcatgt
     8401 tacggagctg atataaatgt attaatgatg actaatttgc attacaaatc gaatgaagat
     8461 accctaaatc tgcttttaag taagcatcgt aatataagtg ttttttaata ttagaagtag
     8521 cattcagaga atgagggtat tatgtttaat aaatttttag ttacaacact atccatagtc
     8581 acattgacac tttcaccata tagttttgcc aaagatgatt catgcgaagt ccaagtagct
     8641 gtttccactc cccctgttca atttgatcaa agtgtagcgt ttaacgtaac gaatgatgat
     8701 gggattagta aatccattat tttacaaggc ggaagcagtc caaaaactat aggaaaatta
     8761 atttgttctc cactgcctta taccattagc gccacccaat attttacacc aacaggctta
     8821 accaataagc atgctcaacc tgttggccag tgcgtattaa aagctggaaa tgtcatattg
     8881 aatggttcta acaatagcgt tgctgttgtt ttccccaatg attttatatg ccaccactaa
     8941 aatattagct gcattgtgcc ttgatgggga gtgcaaaaac atcactccct ggtatatacc
     9001 ggtacgtagc ctttcaattg ttgagcaaac tgtttcagca catcaagacc cgattcctct
     9061 gcctggcggc accattgctg cagagcttct actaattctt tttgagtcgt tgctgttttc
     9121 agccaaacat tttgtaatga ttgcttgtaa gagtaaacca ccctcaactg ttcgcgggca
     9181 tcgagcagtt tttgcaaacg agttttagct tttgtcgata atagcttatc ctctcgacgc
     9241 agtaatctac ctgcccgttg aaacaatttc ttgtcttctt ttgtctcaat gccggtattt
     9301 ttttcatact tcaaaatagg tctgatgaca ttcttatagt aatcagacat cacttgaaag
     9361 cgatttgaaa taactgctct cacagtatcc agatcaacat ggaattttcc ttcatccata
     9421 gccaatttgg gaggtaattt ttttaccttg gccaaaccga aaaaggaaag aatgcgaata
     9481 tacatccaac caatatcaaa ctcccaccat ttcactgaaa atttggctga ggaagcaaaa
     9541 gtgtgatgat tattatgtaa ttcttctcca ccaatccaaa atccccaggg aatgatatta
     9601 gtagcagcat cagggcattc aaaattcctg tatccccaat aatggccaat gccattgatg
     9661 actcccgcag caaacagcgg aatccacatc atttggattg cccatactgt cattccagga
     9721 atcccgaaga aaaataaatc cagtaataac atcagcaaaa tacctttact tgaatggcgc
     9781 gagtagacat tacgctctat ccaatcggtt ggtgtgccat gagaatattt ggcaaccata
     9841 tccctgttct tgcgtgcctc tctgtaaagt tcagcgcctt gccaaaatac cttcctgatt
     9901 ccctcaacca ctgggctatg agggtctccc tcaacatcag ttgttgcatg gtgcttgcga
     9961 tggatagcaa cccaatctgc agtaaccata cccgtagtta gccataacca aaaacggaaa
    10021 aaatgactga ccacagggtg taatgttaac gctctatgag tctggttccg atgaagatag
    10081 atagtaacag atgcaattgt gatttgagtc aggataaaag ttgcaagaac atatccccag
    10141 aaagacaagt ttaaaaagcc gaataccata ataaccccac attgtacgga attataaaaa
    10201 taactataat ttaagagtga atgtatttta ttcctaaatt atagaatcaa tatatgtgta
    10261 atgataacac attttttatc atttcattgc atgatttaat gatgtagtcg ataataaatt
    10321 tactcatctt attttatcag gatatagaca tgagcgataa agttcagaaa ttgattgaaa
    10381 atattgtgcc ttttatagtc attggtgttg ggatcgcaat agttatagcc ttattgttca
    10441 tgttttttta tgtggccatt tggggcgtaa tcatcggtgg aatactctgg cttggagcct
    10501 tagctaagga atatttcttc cccagcaaat catcccagaa agaacaagga agaattatag
    10561 aacatgatga taaaaaataa tgcctgaatt acccgaagtc gaaacaacaa agcaaggtat
    10621 taaaccccat cttgaaggcc gtatgattac cgcggtgcaa gtgcgtaatc gaaaacttag
    10681 actaccagta cctctaaacc tgaatgaatt atgcgaagga aagcatataa cagctattac
    10741 ccgtagagga aaatacatcc tacttcatat ggataaaggt tatcttctca tccatcttgg
    10801 aatgtctgga catttgcgta tcgtctccca gacagcaaat cctcaaaaac atgatcatgt
    10861 tgatctgcac attaataatg ggcttgctct cagattctgt gatccaagaa gattcggctt
    10921 atttatttac attgatgaaa atccttacca acacccactg ctggcacatt taggcccgga
    10981 acctctgtct gatgacttta atagcgagta cttattacgt aaagctgcga ataaatcgca
    11041 atccatcaag tcatttatta tggatagtca aattgttgtc gggataggta atatttatgc
    11101 ggcagagagt ctttttcttg ccaaaatcca tccaaatacc tctgcaaaaa agataactac
    11161 agaagaattc aattctctga ctggtcacat caaaaagata cttgaatctg ccatagaggc
    11221 tggagggacg acacttcgtg atttctattc ttctgatggt aaaccggggt actttcgttt
    11281 tgctttaaag gtttatggca ggaaaaatct cccctgtttg gtatgtgaaa ataaaataga
    11341 aactgtcgtg attgccgggc gccattccgc attttgccct cattgccagc caatcattac
    11401 ataataagtt taataatggc cacctggaac catttttgtt tcttaattga ttctgttgtt
    11461 gtgacaaaag gttaatttat ggatattaaa aaacttaaaa aagcagcctc taattttctg
    11521 gaaagatatc caggcggttt ctaccaccct gaaataattg aaatgggtaa aaaacataaa
    11581 atggataaga tggttgcttt ggctcaagga agttttgcca agaaaaattt taaaaaccct
    11641 gatgaaataa caaattccat gttgaaaatt gttactcagt catctttgat ttcaatattt
    11701 gaaaaaccta aatttagaga ttttatcaaa agaactcccc caaacgataa agtacttatt
    11761 gcaaaaggat taaaagaact tttgtatggt aatgaacagg aaggctttga agagcttctt
    11821 tttcaatttc aaagagagaa acttgcgaag tggactttaa tgactatttt tcaggcttac
    11881 ttcaatcccg aagtggatgt gtttataaaa cctacaacaa ccaaaaatat cattaaaaaa
    11941 ctgaaccttg atatcgtata taacccaacc ccttcctggg aattctatga atcttatagg
    12001 aatatcatta accaaatgaa aacaaaaatt gaaaaatcac tagcgcccag caatgcggca
    12061 ttctgtggtt ttttaatgat gagcctggat aatcgttaaa ttttctatga gtaccgcttg
    12121 atgggctcta tgaaaacatg tcacattctt ttattgagcg ggtgatggga atcgaaccca
    12181 cgccatcagc ttgggaagct gaggttctgc cattgaacta cacccgcaaa aactcacgaa
    12241 ccctttcgtc aatagaatat taaggtacca ttttgtaatt attgattcaa ttaagttatc
    12301 acaacggcga tttaaattta aaaacaggtt ttaaaactca actattaact taaactatac
    12361 aaatgtatgg gaagcgtaac tcacagaaca tagcttggtt gcttcttttg attacaatct
    12421 tatattgata tctaaataaa gtaaaaaata gttattctgt agactaaact ttacagcttc
    12481 aagaaaatca actaagacct aatatgaaat ctctgttatt gcaatctgtg gttgaaaaat
    12541 gaatccgata agaaaaatta tccatattga tatggactgt ttttatgcgg caattgaaat
    12601 gcgtgatttc cctgagctgg caaataaacc tatcgctgta ggcgggaatg caaaacgtcg
    12661 tggagtcata gcaacctgta attatgctgc ccgccaattt ggcatacgct ctgcaatgcc
    12721 aacagcccat gcgcttaaac tttgccgaga attaatctta cggcctgtcc gtatggatgt
    12781 ataccaaaag gaaagtcaat atattagatc cctattaaca gaatacacag acctcattga
    12841 acctttatca ttagatgaag cctatctgga tgtcactgaa tccacacaat gccagggaag
    12901 cgccacctgg attgctgaag aaatacgcgc ccgaatttat caaacaagac aactcactgc
    12961 gagtgcgggc attgcgccaa ataagagtct cgcaaaaata gccagtgatt ggcataaacc
    13021 gaatgggcaa atggttatta gacctgagga cgttagcgca tttgttcttg atttaccagt
    13081 acgcaagtta tttggtgtag ggcccaagat ggaggaaaaa ttgggggcgc tcaacattaa
    13141 aacctgtgct gatttgcagc gatattccat tgaatattta ttacaaaaat ttggcaccat
    13201 gggtcaaaga ctttatgaat tagcgcgagg cattgataat cgccccgtca atcctgagcg
    13261 tattcgaaaa tccatcagcg ttgaagaaac ctatccaaaa gacttgccaa atagtgaagc
    13321 ttgcctggcc gtcttgccag atcttatggc gcgccttgaa gcaagaatac aacgtgcagg
    13381 taaaatttct gggattcaca atttatttgt aaaattgaag tttaatgact ttcagcaaac
    13441 aactattgaa cgagttatgg ataaaattga tttaatcgtt ttacgtcaat tgatacaaga
    13501 aggttttgcc agaagaggca tgccagtacg attattaggt ataggaatta aattgaaaca
    13561 agaaaatacg tatcaaagcg tacaattgcc attacttgac ttataacaag tatctaaaca
    13621 gttgatatca gattctttcc caaacttgcc ataattatca gaacttaata catcaactct
    13681 cttttttatg cgacaccctg caatagttaa caaaatcagg acttttatat tgaaaatatc
    13741 aatcaacgga tgcacgcaat ctagttttca taacataaac aaatagatag gataaattta
    13801 aaaataatct tattattcct gcatcataac acttggaaat attattttga tgcagccatt
    13861 cattatcgac gaaaaactgg tttgtcgttt aattgcgagc cagttccccc attattccaa
    13921 tctccctgtt aagccggtag ctgtcggcgg atgggataac cggacgtttc atcttggcaa
    13981 agatatgctt gtacgactgc ccagtgccga gcaatatgaa ttgcaggttg aaaaagagca
    14041 acaatggctc ccccaattgg cttccttgct acctgttcca attccgacac ctttggcaaa
    14101 gggaatgcct ggcgaaggat acccatggaa atggtcgatt taccgttggc ttgaaggaga
    14161 aacgattgtc tctgccaatt tatctaacct gaatgaaatc gcaaaagatc tggcaacgtt
    14221 cctcaccgct tttcatcaaa ttgattcttc cggtggcccc aaacctggga tgcacagctt
    14281 ttatcgcggt ggagatttga aaatttatga tccagaaacc cgtcaagcta tcgattattt
    14341 aaaaggaaaa attgatacag accatgccac tgaaatttgg gaaaccgctc tgaatacctc
    14401 ttggcaaggc acacctgtgt gggttcatgg tgatatcagc gcgggtaatc ttctggttaa
    14461 aaatgggaaa ttatgcgctg ttatcgattt tgggcagttg tccgttggtg atccggcgtg
    14521 tgatcttgct attacctgga cattatttgc aggagacagc agaaaaatct tccgtgaaat
    14581 gcttgcttta gacaaaggca cttggttccg tgggcaagca tgggctttat ggaaagcgtt
    14641 aatcgtagca gcaggcattg taaacaccaa tgcaatggaa gtgcaaaaat cctgccgtat
    14701 tattaatgag ctatttttga ctgagctata atctatacca tatcgttcaa tttgactcag
    14761 agaatactat ttccttgtag gcagtgatat gctataaaat aatctccgga cgagacgcgc
    14821 cgtacccggt taagacaaga cgacgccttt aatcaatcta aaaggagtcg ttatgctact
    14881 accctggctt tatttaatta tcgcggggct cttggaaatc tgttgggctg tcggtttaaa
    14941 atataccgat ggtttttcga aatggcagcc aagcctgttt acagtcacca cactgattat
    15001 cagtatgttt ttattagcca aggctgccca aacattacct atcggcactg cctatgcggt
    15061 atgggtaggg ataggttcac tggggactgc aatcatggga atactgctgt ttcaggaaca
    15121 tctatctgtc ttccgattgc tataccttgt attaatgctg atatctatct ttggtttaaa
    15181 atacacggct tgatttgaat cgttccagta ttcctcaact tgttgtatga tggccctctc
    15241 ataatcccat gcgccctaac gatgaaaata agtcgattaa aaactttttg gataattacc
    15301 cgcctatttt actacacagg tgttgcttgc actcgctcca tatttaagta tctttttagc
    15361 actcctacga ggccatgggt tgataaagtc atccaccatt gggttgatca actcttaaat
    15421 gctgtgaaag tagaatataa agtaatcaat ccacataatg tccagcctca gcctggcaag
    15481 gcaacgatcc ttatgtgcaa tcattccagt gcttatgata ttcctttagg cttcaaagca
    15541 ttccctaata actctattcg catgcttgcc aaacgagaac tctccaaaat tccactttta
    15601 gggaagggaa tggctgcagc agaattccct tttattgaca gaaaaaacag atttcaagcc
    15661 ataaaagatt tggaatttgc ccagcaactg atggaaagtg gaattattct atggattgca
    15721 ccagaaggaa ctcgctcaaa agatggcaaa ctagccccat ttaaaaaagg tggttttatc
    15781 actgctattc aagccaaggc tactataatt cccataggaa ttcgcggagc ttttgatatt
    15841 ctccctgccc gcacctttaa tatacatttg aatcaaaaag cagaaattca tattggcgag
    15901 cctattgatg cagctcaata tacaattgaa aataaagagg aattaatcaa aaaaacccat
    15961 caatcaataa aaaagctggt tgaggagaat tgaaaaaact cacatcccgc tttgctgaaa
    16021 aaagatgact aaaaatacct acaataacta gtaaaaaaga ccacgttata caaaaaataa
    16081 aattgatttc tacaatagaa taaacaatcc aggctacctg tcttttaata tcggtactaa
    16141 ctgttttgaa atttcctaaa acttcaccta gactttaaaa agaaaatcaa tatgaccgtg
    16201 ccatgagcat taataaaata gaagaagaca gggatacctc ttctgatctg gaaatgaaag
    16261 ccaagatgta cttagtacac cgaatggata aagccgggcc attaacagtt aaagatgttc
    16321 acatcctgat aaacaagtta agtaaagatt tgcagaaaaa tcaactaaca ttaattgatt
    16381 gtcaactaat aataaatctt gttgaatctc gctttacttt ggccaaacaa gcaacaagat
    16441 atctacagtt ttttctaaat aaacttgatg gcaaatcaat taacccaatc ttaatcccac
    16501 tttcaaataa acccttttgg caaactgaac cacatccatt cgagcattat cgatctaccg
    16561 atcaactacc tgaaaaagca gatatcgtaa tcataggagc aggtctaact ggcgcttcta
    16621 ccgcatatca tctgatcgat gctgtcaaga aaagaaatct tactgttgta ttgttagata
    16681 aaggaaatcc agccactgaa gccagtggtc gcaatggggg taattttgaa ttattacctg
    16741 aaaactctgt gggaatctat agtggtttag tctcagagcg gtttcgtttt ttaaaacgat
    16801 gctaccccca tgttcatccg tcaatattac aaattgaaag cgaataccat gcctcactgg
    16861 tttttcaatt tgctttaaaa aatcgcaatc gctttaagga aattgtcaga aaagaacgta
    16921 tttattgtga tctggcagca aaaggatggc tttacattgc tcatactgaa gaggaagaac
    16981 aagcaatttg tgatgaagtt actctggctg cccaacatgg cgagcgcatc tacatttggt
    17041 ctcgaaaaaa aattagagaa caattcggca tcaactccga gttcataggt cgatttattc
    17101 cagatgacgg cacctacaat ccctttaaat atacgtgctg tctgattaag gcggcaatca
    17161 aaagtggtat caagctctat acgtttgtga aagttcaaca aattaagagc atggatacag
    17221 aataccatca attgaaaacc aatatgggtt tgcttaaggc tcggcaagtg attgttgcaa
    17281 caaacgcatt tactagcgag ttgctcccgg aattgagcgc gattaagcct caccaaagtc
    17341 aaattgcaat taccgattca acaccagatt actgtaaggg tcgattaata actagcgaag
    17401 atgggccgct ctatctcaat caacccagag tcagatcaaa gaatgggttg gtgccattgc
    17461 ttattggtgc cggtgacgac cgaccaatga aaaaccctta ttatcgtaag cgttcaaaaa
    17521 aagttcatga tcttattgtg cagcaaagag ataagtattt cccgaacctt aaagggaagc
    17581 cattttcaac cgaatgggtt ggcgccttag cttttacacc tgatcaatta cccgctattg
    17641 gttacctgtc tccagggata attattgtga tgtgctgtaa tggatatgga ggaagctata
    17701 ccacagcagc aggacttacc gcagctgaaa ttgcattaac tggaaaaaat cccccatggt
    17761 tacccgcaga ggtattttct cctaaacgcc tcctgtcaga caagccgcta ttttgggaag
    17821 atagtgacag tttatggcgg attggaaaaa cactttgtac aacacttaat aatctcaatc
    17881 aacttctggc tgagtgcctt tcatatcagt ccatgtatca accttattct acaatttctt
    17941 cattacccca tgggcaccaa gagaaaactt gcaatcttga cccactaaga gtaatgcatc
    18001 aattagcaat ctttagaact tttgcctatc aggaattaga aatgttaatt cgctatacct
    18061 cgcctgtttg cttaagtaag ggagaaattc tttttaaaca aggtgatgcg ggacattctt
    18121 gttttattct gattcaagga aaaatcaatc tttattatga gcatactgaa ggctttactt
    18181 caatggttgc agaactggag gccgggtcag tatttggtca aatggcatta tttcttgaga
    18241 ataaacgtat ggccacttgc caagctacag aaaactgttc catgttagaa atcatggtag
    18301 aaccttgtaa atcgcttttt atccagcaac aggctcttgg aattaaatta ctccgcctct
    18361 taaaccaggg agtcatcgat gcgttacaca aactcaataa gcgattgaaa tacacttaac
    18421 aaaaatcata aaacaaatat tgattttgat tgctttattc agcactaaca atttacgcgc
    18481 caagtgtagc tggccaattc attttaaaaa tgtattctag taattaaccc catacccata
    18541 tgccatatta gatatcgcag cagctaaacc aatatgtctt ggatcagaag cagccgttaa
    18601 gacattactt tctttgtccc atgtaatggc ttgcatatct ccataatatt gtttcaatgg
    18661 cattaaatgg tatcccatag cttgtaaacc ctgttgaatt gaacttggga aagtatcagg
    18721 ctcaaattgc aaaacatctg gtaaatattg atgatgaaat cgcatggctg aaaccatact
    18781 gatagctcca tacccatcat aaaataatag tgaggcaagt aaaaccatag taggaatacg
    18841 gcttccccca ggagtcccca ggatagctac acgccctggc atctccagga aggttggagc
    18901 catgctggat agcggccttt ttcttggttc aatggcatta cgctcacttc ccactaaacc
    18961 aaagacattt tgttcaccca ctttagtaga aaaatcgtcc atttcatcat ttaataatac
    19021 ccctgttccc ttcgcaacta cactggaacc aaaaatgtaa ttaattgtca ttgttgcagc
    19081 aacacgattt ccttccgtat caataatcga aatatgtgag gtatttgagt gttttcccgt
    19141 tggaactgag ccttgcaaga ctttactatc aatcgcttta tttggtgaaa tcaagcgact
    19201 taattgtttc gcattatctg aggaaatcaa tttatcaagc ggaaccttaa taaaatcagg
    19261 atcacccaga aattgatctc tttgccaata agccaatctc atcgattcaa cgacgtaatg
    19321 aacccatttt aatttgggaa atgaagacaa aggaaatttg gataatatat tgagcatggt
    19381 taccaatgcc actccaccgg ccgaaggcag aggagctgta ataattaaca tattatgata
    19441 ggcactaatt aaaggtggcc tcactttaat tttatattga gccagatcat caagagacca
    19501 aattccacct gccgcattga caccttgaac tagcagagaa gccgtttctc ctgaataaaa
    19561 tccggcattt ccgtacttgg ctatcaacct gagtgttttt gccaaatctt tttgaacaag
    19621 cttatctcca atttcaaaag gctgaccatt ctttaaaaaa atagcggcgg tatcgggata
    19681 tttcttcaag ttttccaatc tatcatccag agtggagaaa taacgaaatt gaggatctac
    19741 aggaaaaccc tcttccgcaa gcttaatagc cggagctaat gtcttgctaa gcggcagacg
    19801 tccatagttc ttggctatat aaaccagggc agctggctgt ccaggtatag ccgcagataa
    19861 accaccattt agagaaaggc cggggatcac ttggccgtca ggagccaaat acatatcctt
    19921 acttgcggcc agaggagcta cctccctacc atcaataaag aggtttaaat gttccttttc
    19981 ctgatgaagt agccaaaaac ctcctcctcc aagtcccgaa tgatatggct caactactgc
    20041 caaggcagca gctaccgcaa ctgcagcatc aaaagcattt cctcctgctg ccaaaatctc
    20101 taaaccagca tgagttgcca aggggtgggc acttgctaca gcatatccag gcgggtgttg
    20161 cggaaatgtt tttgcattaa cttgataaat attacaaatt aaaaaaaata aacaactcag
    20221 ccatttataa tgatctttca ttttgtctct tttttaattc tctaactaca ttgggtggaa
    20281 caaattgaga aatatcacca tttaaggctg caatttcacg aactaaagtg gatgagatat
    20341 acattaaatg ctctgcgggg gttaaaaata atgtctctac ctttttagat agttttctgt
    20401 tcattccagc cagttgaaat tcatattcaa aatcagaaac agctctcaat ccacgtaaaa
    20461 taatgcctgc gttttgttct aatacaaaat caattagcaa attatcaaaa cccacaactc
    20521 tcacgccagt taaatgtcca acggactctt ccagtaaact aattctgctt tcccaagata
    20581 aataaggccg cttgttttta ttactcgcta cagcaactat taattcagga aaaatagtgc
    20641 tggcccgagt aataatatca atgtggccat tggttacagg gtcaaatgtt ccagggtaga
    20701 ttgctttttg tttcatctcg ttaattacca tttgcatata gaagcagtct agcgtattgt
    20761 aaattgaaaa actataaaaa aatggaattg aggtaagcaa tgaatgcgat aaaacgtttg
    20821 acaatcattt taatttgctt tttaacagca tgcgctcccc caagaccctc tgaagagccg
    20881 cctgcaaacc gtgaaattcc tcttgaagaa cgtaaagccg aaacagccac tgtttcctca
    20941 tgggaaatta aaggggctct ggctgcaaaa accaaagcga aaggatggtc tgctactatg
    21001 aattgggtcc aacaaggggc caactcatac caaattcgtt taatggggcc attaggcggt
    21061 ggggctgttc taatcagcaa aaaagggaat actattacat ttcaagatgg ctccaaaata
    21121 actacttcga ccaatgccga tgagttatta ctaaaacaaa ctggcattcg acttccagta
    21181 aataatttgt attactgggt aagaggttta cctgcaccag gtggagtcca atcagaaaaa
    21241 catgaccaat tcaatcacct gatacaatta aagcaaaatg gctacaccat tgattttaat
    21301 aagtatactt cggtcaaggg aatagattta ccaagtatga ttcgcctgga aggaaatggc
    21361 gtcatggtaa aggtagtgat aaagaattgg gttgtataat ttaattcttg taatttattt
    21421 gctttctgaa gttgacatcg ataaaaaagc actgcaaaat acgcagcaca ttgcagggat
    21481 gaatactatt tataaactca tggatgattg aagaatctac aattataggg gtgtcgccaa
    21541 gcggtaaggc acagggtttt gatccctgca tacctaggtt cgaatcctag cacccctgcc
    21601 attttcttgt tgattcaacc tagaatatga agttgaatct aggtacaagt aaacagacgg
    21661 cagaagataa cctggaagga tgcgtacttc gtatcgctca aataataata atatcttttt
    21721 ggctgtcttc tgctatgtgt ttaagttatg tgattaacct aaatctaagg cttttgacac
    21781 atgtccacga tgatgatttt tacagggaat gctaaccctg aattagcact aaaaatttcc
    21841 tcacatttgc aaatacctat tggcaaagcc accgttggta ccttcagcga tggagagact
    21901 atggttgaaa ttttagaaaa tgtccgaggt aaagatgttt ttgtcctcca atcaacttgt
    21961 gcgcctgcaa ataataattt aatggagttg ctgattatgg cggatgccct gagaagatca
    22021 tctgctggtc gtattacagc tgtagttcct tattttggct acgcacgcca agataggcga
    22081 gtgcgctccg cccgtgttcc tatcacagct aaagtggtag cagacatgat ggcttcagtt
    22141 ggaatttgtc gggtccttac tgttgatttg cacgcggatc aaatacaggg tttcttttat
    22201 atgccggttg ataacgtata ctcaactccg gtattgctgg aagatataac taaacaaaaa
    22261 ttaaacaata tcatgatagt atcgccagat gtcggtgggg ttgtacgtgc cagagcagtt
    22321 gctaaaagac tgaacgacgc tgagctatcg attattgata aacgccgtag tgggccaaat
    22381 aaatcagaag taatgcacat tattggtgag cccgccaata aaaactgtat tattgtagac
    22441 gacatagtag atactgctgg aacactttgt acggctgctc atgaattaaa gaaaaacgga
    22501 gcaaagagcg tcagagctta cattacacat cctgtgttgt ctggccccgc agttaacaat
    22561 attaaacatt caggtcttga tgaggtggta gtaactgata ccattccttt atcagccgaa
    22621 gcacaaaact gtgaaaaaat cagagtggtc agtcttgctg atatgttagc tcaagcaatc
    22681 aaacgagtaa atgttgagga gtcagtaagc tccatgtttg ctgaataaaa tgaaatattc
    22741 tttgataata aaaagggagc cttagctccc tttttattat caatctttct taatcccttg
    22801 taccaacgta tttatcatca aaataacgtc ttaatttagt tcttaaagtt ccacgactaa
    22861 ttcccagcat gattgcagcg cgggactgat tatacttgca atgttccatt actgcacgaa
    22921 atagaggggt ctcaatttct tcaagtacaa actggtataa gtcaacagga tcagttccct
    22981 taagctctga aaaatatttt tgtacggatt gagtcacgct ttcggctagt gaagcagtgg
    23041 tatctcctgc atcattactg attgttgtca ttattattag ctcctccttt taaaagcggt
    23101 atattaccga attgagcaat acgagacaag ggcagaacag aaaaaactgc attaaaattc
    23161 aatatatact gtttgctctt gaatcattgc cacgtttctc accatagtgg attgaaaaga
    23221 ggataagccg ctctcaacag cttatcctat atctaacgaa ctactgaaat ttcatcatag
    23281 atggatcaaa tggtttccca ttaacagtga attttccttc actcaaactc atctcaatga
    23341 catagtcttt gccatcgaca gtaattaaac cagattgctc caactcatta atttgcttat
    23401 ctacttgcat tgcagcgatt tgctcaggag taggttctgc ttggtttgat tgaccttgac
    23461 tgttttgcag ctgttgaatt aacgcttgtt gcatttctgg ctgttttgcc atttgttgca
    23521 taatcgattg ctgcatcagt tgcttcacta cctcgattgg catttttaac ttggcattac
    23581 cttgagtttt ttgaatcaat tcaaagggat tagaactatc tcctttgggt aaagtaatta
    23641 acaagttacc atcaatcata ccttgtggaa gtttgagact taatttggaa atttcaaact
    23701 ctgcaccttt actaaataac tttggcaatt ctggcagcat tgccatcaat gcttgttgac
    23761 gctcttgatc tgtgcccctt tgcatcgcat tagcttgctc attgatttta gccaatactt
    23821 cagcatcaag gtttcttaag gacatctcca cttcaccagg accgtaattt tgcccattgg
    23881 cgaatagtga ttttaaagca gcactaaaat gcgtgctgaa gagattatcg tctatatcgc
    23941 tgcttgaact taatgtcaat tcactaattt caaacatctt ttgatcttta acaactacag
    24001 caagtgatgg caatgaaaaa ctggcttgac ccaagtataa ccctgtagtc gttttatgta
    24061 agtcatactc gctggttacc ttacccaaag agagcttggt atcatcttta gaaaactctg
    24121 cgccatcaaa ttcaatttcc ccttccactt tatccaaatt ggaagacatt gatgttgtac
    24181 tgtccatacc catccaatta aatttaccat ttccatcttt agcagttaat ttgaaagaag
    24241 gaatggacaa ctcaactgta cttttattca aataatttac aaaaatgctt aaatccagct
    24301 gaggcttttc tgaatcttga gaaaaaagct cattgaattg ctgattatat ttttctggaa
    24361 atggaaatac ggtttgtgca aatcccattc caaaacgtaa ctgcttattc gcgtatataa
    24421 tagggccatg ataaatctta atcggcattt ccatttgata atcttgagca ggagtagttt
    24481 gcaattttcc gtcatcgtct tttactactc gctcaggtac gtgtaaacgc catttaacta
    24541 atgcatcaga gcaaaaccaa cctctcttat attgctgaat gtcagcgaaa agcccattgg
    24601 attgattaat tacctcgaca gtttttttaa ccgttctttc ggttagaatt cccatgccat
    24661 aatatccacc taggattaaa acagccagga taattactaa tcctgtaagt tttttcatta
    24721 ttatctccat gttattaatt tgcaaatagc attactcgag cgcagcaaag tgctgcgaaa
    24781 agtataacag caaataacct aaaagtgcga cagtctaatg caagaagatt aaaacttaat
    24841 gaaaaatatt ttcattaaat ttcataaatt tgatgtattt acagcaaaaa tcaaacaatg
    24901 gtggcatatt tatcatgcaa tgctcaatgt ttgatttttg ctgcctctta atgccttatg
    24961 ggtttgcaat aagtctcttt aacagtaaac gcaaggatta aggccaccaa ggaacataag
    25021 gggagtaatt ttagaccggc ttggaaatcc gataataata acgtttcaac attataggct
    25081 ctgggcccag acaccatgtc tatgatacgt cctactaaag gctggaataa ggccccgaca
    25141 aaaattgaag tcatattggt aaatgcgata gatgtaccaa caacactcct gtcatgtatt
    25201 tctgtagaaa cagcgtatga aatcgcaact ccagtgtttg taagaccaaa aacaaaaaat
    25261 agtatgtaag ccgttgtttg actcatttca ggaataaaaa caaataaact gctcaagatc
    25321 acaccacata ctgcggaaat aatcatcaat ggcttacgac gtcccatttt atcagaaatc
    25381 caacctgata atgggccact aattccccag cctataaaga tcaatcctgt tgcaaatgca
    25441 gctgcgtgtg cccctaaacc acgcccgaac tgtaaatatg ccggacctat tgcctctcca
    25501 attacagcgg tggggccaaa taaaaaccca gcatacatcg cattaagcca agtttgttta
    25561 tttgataaaa tgatctttaa gctatccaaa atactgattc gattaacaga acgaatttca
    25621 ttgcgatgct caccgggttt atcttgcaca aattgataca ataatcctga taaggcaata
    25681 aataagaagg cgataatcag catgctatgg cgccagccaa cattactcac taaaaatgaa
    25741 acaggagcct cccctgcagc agcaccaagc attcctaacg cttgagttaa tccagataat
    25801 aatccaagca tagttggggg aaaccatgaa gtggcaaggc gaagtgagca aatgaatgca
    25861 aaagcggcac tgaaaccaat caacattctt ccaattgatg cagtcagcag accatccgca
    25921 aggccaaata cacagcaacc aaaagcagta actaatgaca taatagtcaa taaccatcgt
    25981 atgcttaatc gatcaactgt caatcctaca ggaatttgca ttaagatata tggaacatag
    26041 aaggatgcgg ttaatatgcc gaagccagcc tcattaatgc caaagtccat ttgtagatat
    26101 cggtgcatta caccaggcgc tactctagct aaataatcag aaaaataaaa agcagctgcc
    26161 aacccccaaa ctaaccacgc aaaacccagg taatttttac gtttataaat ttccacttta
    26221 gatattccca ttttggtata accaccacat gctgtattta tttaaaaaat gatcaataaa
    26281 ttaacaatga taacttatca ttatactgct gttgtaactt atttcaacat atagtttatt
    26341 tttacctttg ccccattagt aatgtattga ggatttagga tataatccta atttttaatg
    26401 aatcatcgtc atccagcaaa acaaaaatcc tctggattga gttgaatatt aaaagaccag
    26461 gacttacgaa aaaatccaag gtcgaggaaa aaaatgtttt aaatgagagg ggttagacat
    26521 actagatgac cgaattaaaa atatttttta atcaagagat tggagttttc ggatatccag
    26581 tggatgaact caaaacaaaa ctgaatcatc cccgagggtt caccaagaag ttttaaagag
    26641 agtaaggcaa aatcatattt tgattatata taatgaaaca ttcgagtcgt tccgcctgtg
    26701 cggatgacaa aaataggttc aaatttataa agtaccaggg ctcataattg accgaaattg
    26761 gtatgccatt gtcctgttaa aattgaatcc tcatatattt tcatgaatgg aagtcggtta
    26821 ctctgaacaa gtttaaaaag caaattttca agatactata cagacatgag atcccccctg
    26881 atgaacaaac taaatatagc cattaaagaa gcttttgaat cttccggaag ctcaaaagaa
    26941 gcgaataaag tttaccttga atttatcaag gcaaatttta ttattcctat tgaatccaat
    27001 tctgatgata gtgaacccat agtattattt ttggaagaaa ataaccatgt ttttttgccg
    27061 gtattcactg atatgaaata cctcgatgct tgggcaagtg aaattagcca acatatcaaa
    27121 ttacttaaat tatcaggggt tgacctactg aaaggtttag gtgagaaagt tacagtttgc
    27181 cttgatattg gcagtgagct ttataaagaa ttcaatccat ctgaaattga acggatgagg
    27241 tcgatggttt taaaattatt taaaaactga actcaataca aggaacactc ccattcaatt
    27301 gaagaagcat tatattatgc tcttcaattg aacgcgatga tcatgctaca agctcaagta
    27361 attggtagcc tactattgcg gaagcggcta tcgcaccacc aatttcaaag actaaagata
    27421 ggaagctgtt tttatgatga tattgctgat catttgcagc tgtggtttta tctaaaagaa
    27481 tatcttcctc gatattgttc aaatcaaata atggtttttc tccatatata ccagcccacc
    27541 atccttcgag ccattgttct gcttcttgag aaccttcgcg gaaaggatta tcctcttctg
    27601 gaacttctgc caatgcacat tcatacccaa aaatataaca ttcttcatag cttgggtgtt
    27661 caatgttaaa tcgtaatcta agatgtgata ataatgatgt tgtatcgttc atgatgatag
    27721 cctcttttat gtatcctgaa tatatttccg cttttcaact catttactcc taagtctata
    27781 attattaacg caattggtgc agaactgctt tttagtgcat gaaactccca cacttaaatc
    27841 aattttacga ctttaatttg ccccctgcct tcgatgatca aatatttaca ttatcttgac
    27901 aaaaatcttt ttacctttgg ttct
//
LOCUS       DBNCWK010000025        26679 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.025, whole genome shotgun sequence.
ACCESSION   DBNCWK010000025 DBNCWK010000000
VERSION     DBNCWK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26679
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.025"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(67..810)
                     /locus_tag="AC06IO_001011"
     CDS             complement(67..810)
                     /locus_tag="AC06IO_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIO9874217.1"
                     /translation="MVRLRIYLCSLLLVIPFYGAYALDLVIGTLSYNPPFEIQVNNPS
                     KENEFYGFEIELMDEICERIHAKCQYKPLLFEQILAEINSGAINLGIGSINITPERSE
                     RFLFSLPYKSSYHQFLIPSNSNIKKIDDLKGKTIGIHLGSPTADYLTTYFNNDIQFKY
                     YQTFMDLLNGLTDPQVSAIVTNSDQAQYWASSTPDCKLLGDSFPFGIGFGVVARLDQA
                     ELIEKINQALIEIENDGTYLKIYNLFFQP"
     gene            complement(972..2495)
                     /gene="ankQ"
                     /locus_tag="AC06IO_001012"
     CDS             complement(972..2495)
                     /gene="ankQ"
                     /locus_tag="AC06IO_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkQ/LegA10"
                     /protein_id="HIO9874218.1"
                     /translation="MIMAATIPHIETITFNDWFLAIIDTPGIPIDEIETRAAEGFKRK
                     LTERQFQQLTELIHLISFIKHRRFSNHTLALRIYVDENTSSLSRTALVEAVRMLPPED
                     NKTYELVAYLAQKNKLERYTNITKVPPLQIYQGDGVFEQYDEWILLFELFGLTQATPS
                     DFIPAIAHLLIIKNLGIPDLRALSKLMSTTHKLGILSQSFMNNMTPHLCNGQIMEKYE
                     SLLLKLQINDLLTEEILEVIYPLLNQLDALDAFFSLYHEELSHDSALSFLREILPPFC
                     AMSLPSKENYDDQVPTNTPLHLAVIESDKGNLERTLAFANRNLLIKCSYENTALLLAC
                     KLADKESARLILAKMQELGCDVNQRDHHGMTALHWANFYHFDELIRELEIAGADPQLK
                     AANGKTCEYFYQHQFTLKDLKLNGKEIIDGEFKLSDCALTDIAFHMDKIALNLKLTTP
                     SEVAELYARDEMAQIRSSNRFYLFFKLFRPKLINWLEKQNDLEPQVTYRFSGQNSSA"
     gene            2753..3244
                     /locus_tag="AC06IO_001013"
     CDS             2753..3244
                     /locus_tag="AC06IO_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HIO9874219.1"
                     /translation="MKFRFRFYLGIILLILAIVCPLLIPLIVQSNFSVLIKGFLSSIL
                     VFGLPEVLIVLAIGLLGKEVYGFLEQKIKNILFKEKVSRARYRLGLVFFAFPLVIGLL
                     EVFVKEITLAYGSYYYWVEIIWTTMFALSFFICGKQFWDKFKSLFIYDSYVVFEKNKK
                     CVY"
     gene            complement(3257..4252)
                     /locus_tag="AC06IO_001014"
     CDS             complement(3257..4252)
                     /locus_tag="AC06IO_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIO9874220.1"
                     /translation="MDPITHGVLGAACAQAALGKYDKRIPWQAGALAGMAPDLDVFIR
                     FSQEPLSLEQWHRYLTHSLMFIPIGGLIVALFLLCFSQYRQYWKVTIGASLVGYATHC
                     LLDSLTSYGTVLFWPWSDSRISWDIISIIDPVFTLPLLLGTAWSVIYQNQKAVIASLF
                     FCILFLIFNTILHNRAVDGIHQYATTNNFKINNPRALPKLASSTYWRIIAKQQDCLII
                     AEAKTSLFYPTTIRLIRQALSFNESIIPFALSSGQKSDLATFHWFTDGFLIVANKNPL
                     ILADARYTIDDNPIFSLWGIEILPGQEHINKLSLPKINEYCKSPDSERYKGDGLN"
     gene            4444..4740
                     /locus_tag="AC06IO_001015"
     CDS             4444..4740
                     /locus_tag="AC06IO_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874221.1"
                     /translation="MSSSRFNIAFSNTAGNVGGSDSLNEWLTGISIKAGSITKLTHNI
                     DLLLTYQFTQYNSIAGVQMEPLSEDTLRGSYKPNVNTVLIGFKVHPSDEITKDT"
     gene            complement(5055..5915)
                     /locus_tag="AC06IO_001016"
     CDS             complement(5055..5915)
                     /locus_tag="AC06IO_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HIO9874222.1"
                     /translation="MISYLFSEVHFTHALKKVLGISILVGVYSAIIYFWFEQDNLHSS
                     NWLSYSQSVFGIILGLLLVFRSNRAYERWWEARTLWGQLVNASRNLAIKIRVLLSPNI
                     DEAKKFSGLINTFCEVLAIHLRQKSTRDDFKNFLKMASLPSHVPSYVAQELYRELSEK
                     TKASKRLNLWLLDNEFSKFMDICGGCERIKNTFISLSFRVFVKHVFIIFILFLPCSLI
                     DTLGVWAIPATIIITYLIIAIEGIARNLEEPFGLTEDHLNLGSITESIRESVNEVLLG
                     QKSNEESNSI"
     gene            complement(6093..6329)
                     /locus_tag="AC06IO_001017"
     CDS             complement(6093..6329)
                     /locus_tag="AC06IO_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874223.1"
                     /translation="MKRVHHHPATHSHPSNATVQVSNGQQTVVVNPNPSYLPGQPPMY
                     HPNSFFTTHHHSPYYASQNQHYHQQPAPNTHQHP"
     gene            complement(6532..6744)
                     /locus_tag="AC06IO_001018"
     CDS             complement(6532..6744)
                     /locus_tag="AC06IO_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0045 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874224.1"
                     /translation="MKSTNNMQKKETDKPTSEQAQKKEVRRNNAPDFIPGASPYYNLY
                     CFFYKLCHGEDTSRQHQPAASFQHGK"
     gene            6954..7352
                     /locus_tag="AC06IO_001019"
     CDS             6954..7352
                     /locus_tag="AC06IO_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874225.1"
                     /translation="MNCFPFFAKKNKKNNEEKAEKTLSVFPAGTSINKTTVKEICFTD
                     IFCDTHPFVWYYYPEHNLAIWTSRLAGTTQEYPADYLLNQLAKIPGITIDQGDAAIKD
                     VSTFLQDHQMIPIRNNESWRYIKSSSFSMS"
     gene            7551..8213
                     /locus_tag="AC06IO_001020"
     CDS             7551..8213
                     /locus_tag="AC06IO_001020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HIO9874226.1"
                     /translation="MKPTDKKHWSKIEYLHQSVKNPNIHIKGKHSYYSDAWTGSFEET
                     VVRYLYGDEYSLKTWQPQWQIDQLYIGDYVCIAADVIILLGGNHNHRADWFCLYPFAD
                     KYVEAYQGKGDTIIKDGVWLGMRAVIMPGVTIGEGAIVAASSIVTKDVEPYSIVAGNP
                     AKPVKKRFAENMIERILALGIYSWSEAKFNLLKDYICSNNIDKLEQASRSYDQNHTIE
                     SK"
     gene            8210..9088
                     /locus_tag="AC06IO_001021"
     CDS             8210..9088
                     /locus_tag="AC06IO_001021"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9874227.1"
                     /translation="MINIRKLRLGDESTLEIFLKSHADESMFLRSNLYHSGLSYHDKP
                     FHGDYFAAFDNNKKISGVLAHYWNGNVIVQCPDFNVLTTLVGTFQKAATREIAGILGE
                     DQQADVVMKQLQLEHVDYAVNYPDGLYALNLQNLAMPGNQDRYQLKVAKDCDNCLLYE
                     WLRAYQIEALGAEEDSSILKEQVESEVKSTLEGNDRWVLEENGIPVSLCGFNARLPDI
                     VQIGPVWTPVTLRGRGYARTVVALSLSAAQKSGVSRAVLFTNNQAAVRAYQAIGFNKV
                     GNYRLAILKKPILLTR"
     gene            9141..10436
                     /locus_tag="AC06IO_001022"
     CDS             9141..10436
                     /locus_tag="AC06IO_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIO9874228.1"
                     /translation="MDMDIKHKPLKRELSLFGATMMGLGSIVGTGVFVSIGIAAGVTG
                     PSVILAIALAALVATCNAFSSAQLAANHAVSGGTYEYGYRYLHPAFGFTAGWMFLCAK
                     SASAATAALGFAGYFIHLSGLETVPVIPIALAASITLILLVLSGLRRTNTVNIIIVMV
                     TLITLTVFIISGLPDFMQRGTANLRPFFPVSKQEGLSHFFYATALMFVAYTGYGRIAT
                     LAEEVKNPGKFIPAAIIITLMVSAVLYMLVGLVAIGAVGVEKLAQVTETNATPLESAA
                     RAIGIPGLSVLVSIGACTAMLGVLLNLILGLSRVALAMGRKGDLPKMFCYVSETRHVP
                     VFAVIGVGVVIIALVLTGSVETTWTFSAFTVLIYYAITNLAALRLPKEKQLYPPLFAI
                     GGLLSCLFLAFWVPIGVWITGISLLVLGLLWKVVANRFL"
     gene            10500..11369
                     /locus_tag="AC06IO_001023"
     CDS             10500..11369
                     /locus_tag="AC06IO_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HIO9874229.1"
                     /translation="MIDGIMVLWFILLMLSLAFVAYDVREMPIDWVQKLGWVLVVAYT
                     GPLGLFFYFLTCRSPGKGLHALYTKAQWKQAVNSEVHCLAGDATGIIFAAIFLSFIDL
                     SNGIELILEYISAFFFGWIIFQAGMMRSMYSSYGEALRKTFFAETLSMNFVMMGMIPV
                     MMILMNTLDYGRNPAHLQFWFIMGMATVVGGFLAFPINNWLVKKKLKHGCMTLPEGDE
                     QHIEGHTTHHHGGHEEHLQAQAKRIGSKVVHGHTHEMGSLPLNIQLKYIGISFGVLLI
                     IVLIMAFFVPLKI"
     gene            complement(11430..11582)
                     /locus_tag="AC06IO_001024"
     CDS             complement(11430..11582)
                     /locus_tag="AC06IO_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874230.1"
                     /translation="MFNLKRNLIKLGTLSISIALLAACHTVKGTVTGAKQDIKATGKA
                     ISRTMQ"
     gene            complement(11858..12340)
                     /locus_tag="AC06IO_001025"
     CDS             complement(11858..12340)
                     /locus_tag="AC06IO_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIO9874231.1"
                     /translation="MWLDYLHKINSDPNLLYLFIRTFIIYVFAIFIIRVGNTRYNFET
                     AFDYIFVFVIGAVLSRAINGNSTLISAMAGSSLLIFLHWIFAICSFYSHGFGKLVKGK
                     SVLLIQDGQLIWSALKRHQVTEEDLRQVFREKLNASDLTTVKEARLERTGRISFITYK
                     "
     gene            complement(12550..13443)
                     /gene="prpB"
                     /locus_tag="AC06IO_001026"
     CDS             complement(12550..13443)
                     /gene="prpB"
                     /locus_tag="AC06IO_001026"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HIO9874232.1"
                     /translation="MSNFRYSQGKILREALMKEKPLQVVGTINAYTALQAKRAGFHAI
                     YLSGAGVANASHGLPDLGITTLNDVLEDVRRIMSAVDLPLLVDIDTGWGGAFSIARTI
                     KEMIKAGAAAVHIEDQVQAKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMART
                     DSLANEGLNKALERISAYIEAGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVT
                     PLFTLEELKEVGVSLALYPLSAFRAMSAAAEKVYDTIRKNGSQNDILAEMQTREELYQ
                     VLNYHFYEDKLNELFMKEKTT"
     gene            complement(13752..14486)
                     /locus_tag="AC06IO_001027"
     CDS             complement(13752..14486)
                     /locus_tag="AC06IO_001027"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9874233.1"
                     /translation="MLNKLMWLTGKAQEDWIEKILHLRQKIWPDYQRIKNDTLFKLYS
                     HYPLYQWLSHYNGEVVAVINSLPVALDQSLDNLPQEGLNWVLSHLESTKPLTQSNYLA
                     AVSVTVNPAYQKLGLSKLLLNRLKASAQEAQYKALIVPARPSQKENYPLISLEDYLKW
                     KRVDNTIFDPWLRVHAQLGGKIAYPCQRSRVITANVAQWEQWFGHPLLQTGQYLVKGG
                     LNPVTINKEKDIGIYIEPNVWVIHDL"
     gene            complement(14476..15180)
                     /locus_tag="AC06IO_001028"
     CDS             complement(14476..15180)
                     /locus_tag="AC06IO_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3865 domain-containing protein"
                     /protein_id="HIO9874234.1"
                     /translation="MIREYMDKRNNQFTTEILNYFSDLKKFSTSYLEWITMEHYQFSL
                     NNTRFLMNSHLQTKSLSDPGVSEELLANYNEEKNHAQMYRHALKKIGLDVTNRIPCVA
                     TRQFFMRLEALIEINASKTLGVMYATETAAIFEHILFKEISAELCQRKRLDFENSRIK
                     QFHDLHLDGVEQAHKDELGKFMDHILAEEHVDPNIFLQEKEIMDGAYAAIDIMEDWWS
                     GLINAKAHLLEQAHAQ"
     gene            15286..16194
                     /locus_tag="AC06IO_001029"
     CDS             15286..16194
                     /locus_tag="AC06IO_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIO9874235.1"
                     /translation="MINFSQIQVFITIVESGGFIAAGEKMHRSQPAITNAIKKLESSL
                     GFELFDRKIYRPQLTAKGKAFYQKAKEVVAQFMQLENSAETLRSGVETQFSIDLDIAL
                     PLPGYAPLIQSFIRQYPETCFSLSNNTFVRCLERLERRECHLCIAASHIRSPQIEYLR
                     MPSIRMIPVASRDYYDQHKERINNPKLGIYCMQIMIDGTQQELYQLKIEPLSHSMPKW
                     LVGDMYIRKQLILSGMGIGRLPDHLVEKELAEGSLVQLNTRDYVAVTMDIYAMRLNNQ
                     EHGMVSNYAWQSLKSYAEDTVSPDLK"
     gene            16339..17058
                     /locus_tag="AC06IO_001030"
     CDS             16339..17058
                     /locus_tag="AC06IO_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIO9874236.1"
                     /translation="MIDFKINSQPDDESCGPTCLHAIYQYYGLDISYEEVANQVESFL
                     SGGTIAPMLGKHALQLGFKVTLYVNNLQLYDPTWFNSDGSSNTDVLREMLMQQMRYKR
                     NQYLARGTHAALDFLTLGGAIYFRCLTAEILKEYFARQIPILTGLSSTYLYNCSRELF
                     NKEGKSRSDPIRGLPCGHFVVLCGYNQKHRLVVVADPYKKNPVSGDNYYTVSIHRLIN
                     AIMLGVVTRDGNLLIIQPKYA"
     gene            17213..18211
                     /locus_tag="AC06IO_001031"
     CDS             17213..18211
                     /locus_tag="AC06IO_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874237.1"
                     /translation="MSVIIDSLKNSDVPHLYLLKVGLTRKEYNNTSMMSRDEKRQLVN
                     NIIAKASHEEILKIINDLMAIELSIESTDPIRTGNRLIGQLLLGYITKIDQQNFINFY
                     DQTIKNGNKTLGDYLIPEQVKQIWATIKQTAVKYFSLNHRDADYQAFLNKGFRILPIF
                     YYQQQFPEITPEQYRQGVRPVELTREREEIKNAFHNNLSANVTIPAFPEANYLKTRLA
                     EIKMHIMANEWKLANYSFYSDGVMHGDKRLPHRVKDILDVIEKFESSKLNAKAAYKQI
                     VVKAKEALDYPRSGRFSETTDFYQDIYSHHILRDDYQFNHSRELTSYHGSLFNINR"
     gene            18798..20039
                     /gene="sdbB"
                     /locus_tag="AC06IO_001032"
                     /gene_synonym="sdbC"
     CDS             18798..20039
                     /gene="sdbB"
                     /locus_tag="AC06IO_001032"
                     /gene_synonym="sdbC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector alpha/beta hydrolase"
                     /protein_id="HIO9874238.1"
                     /translation="MTRSKGLKTRSSSFFQNHLKVSVGKLLFSEGVGDWYKKKGYGAQ
                     DTDKDFLAPFADFLNQQKNNPEATYFNAFQSLELTLNRVPSQLVSGDWCELEVLKCEP
                     EADSPQKPGTGKHIVYFPGANTYYQACFRDISAAAKETGATVHALNYPGIGMSTGEVV
                     EANDLTNAGIAVVQSLLKQGVKPDDIVLQGDCFGASVALEVKKEIEKQADIKVRLIMN
                     NAFKSFKAAVSDLIGRSPLIPSISKSIVKNLLEFTGWNVVPGQKYIHSDPYQCYIQHE
                     GDQTLTTATLSSKVAKYKEEIRTGRTTSHSRDPVIDRCPEEYKPYRDFLDESCFVRVR
                     DSAKERLAAKFGVDSRGEVNAHFADLCELEMLGGQSVYEGFVNNYLVMSDKYIKAHPQ
                     KDYDPDKINFLSSNPMGYTPK"
     gene            complement(20342..20746)
                     /locus_tag="AC06IO_001033"
     CDS             complement(20342..20746)
                     /locus_tag="AC06IO_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIO9874239.1"
                     /translation="MNNKKIGEFAKLCGVGIETVRYYQRQGLLHVPLLNKELGAEKIR
                     RYGDDDISRLKFILLAKKAGFTLKEIKELLEFDAKNDRERVRDMAERRIEKLNLKINE
                     LTEARNRLSLLVTKCIETSSSPCPILMTFEKS"
     gene            20816..21559
                     /locus_tag="AC06IO_001034"
     CDS             20816..21559
                     /locus_tag="AC06IO_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HIO9874240.1"
                     /translation="MNHTSIKKAVLYRMVIEHHVCPYGLKARDLLRRQGYEVEDNWLT
                     TREQVEAFKDKHNVKTTPQTFINDERIGGYDDLRRYFGKKVRDPKEKSYRPVIVLFAI
                     TALMAMAVSYTRFANPFTPHALSWFIAFSMAVLALLKLQNIESFSTMFLNYDLLAKRW
                     VPYSYIYPFAEGLAGILMIAGALNWLSIPIALFIGTVGALSVFKAVYIDKRELKCACV
                     GGDSNVPLGFISLTENLMMIGMALFMLLM"
     gene            21759..22850
                     /gene="ceg2"
                     /locus_tag="AC06IO_001035"
     CDS             21759..22850
                     /gene="ceg2"
                     /locus_tag="AC06IO_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0059 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874241.1"
                     /translation="MSKEIKKNLQNNNSAFVYLRSTGLATNSLSSILQYMTRIIVLTE
                     SAGIALGILGVLFSVIGFVSSTIARILDPTASKLSRVMSAGILIAAAAGAIPLIYLGT
                     LFPTMWVYLGIGIMGFGILNSSYYLYSDVRKENALINQYKDNPEQYVESIKEKRQAKY
                     EKLLNTRLDDKQARVLLVFKLYQLDKKLAFAKQEGPARLYDIVCHRKHTRSLERFIDL
                     GTSLTSLIVGVSGLFWGIAAWPIGPILLGISLAVFSVSLSKSAIQFYRKVNKNNALLK
                     EVLSNDRYVSLIDANLDNPYDIEEMDTVEKTQEKVCTRELSQESALSKDQEHKEHYTK
                     LFQPVNKTALRPNDEETDELSCKPVLLQR"
     gene            22892..23167
                     /locus_tag="AC06IO_001036"
                     /pseudo
     CDS             22892..23167
                     /locus_tag="AC06IO_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576214.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(23237..24112)
                     /locus_tag="AC06IO_001037"
     CDS             complement(23237..24112)
                     /locus_tag="AC06IO_001037"
                     /inference="COORDINATES: protein motif:HMM:NF019421.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HIO9874242.1"
                     /translation="MNKEDDEFRISIAGAQEKTAFLYYQNEWHKPFGPTPTTHIFKLP
                     IGYLPHQGIALSESCENEHLCLLLASKFGLEVAKSKILQFEDEKVLVVERFDRKWSRD
                     NRYILRLPQEDLCQALGYSPNLKYQSDGGPEIMQLLNGSQQPQKDRDEFFRAQVVYWC
                     LGAIDAHAKNFSISIQPQGGYQLTPLYDIISAYPIVEQKQLSFKKLKMAMALYGKNSH
                     YKIDGIQKRHFIETAKYAQYSKIKAAQIFEQVIDRIDSAIDEVSTQLPNHFPEKIADP
                     IFKGMRAFKERMIST"
     gene            complement(24227..24430)
                     /locus_tag="AC06IO_001038"
                     /pseudo
     CDS             complement(24227..24430)
                     /locus_tag="AC06IO_001038"
                     /inference="COORDINATES: protein motif:HMM:TIGR03071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
     gene            complement(24526..24765)
                     /locus_tag="AC06IO_001039"
     CDS             complement(24526..24765)
                     /locus_tag="AC06IO_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9874243.1"
                     /translation="MIISSAKDLALYLNDRRKQLKMSQSEVTDLVGLKQDAISKFENS
                     PDNSRIDTLFRILSALNLNISLVEKGQKTHEEIEW"
     gene            24825..25061
                     /locus_tag="AC06IO_001040"
                     /pseudo
     CDS             24825..25061
                     /locus_tag="AC06IO_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
     gene            25391..25654
                     /locus_tag="AC06IO_001041"
     CDS             25391..25654
                     /locus_tag="AC06IO_001041"
                     /inference="COORDINATES: protein motif:HMM:NF036299.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HIO9874244.1"
                     /translation="MKKSLIETVTTRKQSPKRTSARKSKVEFLALREEISEALEKGWS
                     LTAIWETLCDEGSITATYNTFRLYVLKYLNGQLPGYNQKDSID"
     gene            26115..>26679
                     /locus_tag="AC06IO_001042"
     CDS             26115..>26679
                     /locus_tag="AC06IO_001042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874245.1"
                     /translation="MFQISKKYNQDRITISEEEFSILKETSDKIRLALSFERNFEYLI
                     QNYLDYENTIAQKSIGQLVDNLQSIEEFNSHINDVNLKLLNLLSTTKAYIDKSPSILK
                     KLTGEADSFKIQCSLQYDTNFSYRFVDSLRNYAQHNNDPLSSLILGGEWVERENLEYY
                     TNPIIDTEVLRANKKFRKSVLPEYDVKF"
BASE COUNT         8246 a         4786 c         5247 g         8400 t
ORIGIN      
        1 tcagattata gacaaaaatc aatagattta ttaagcacgg gggtcctttg tcggttgaaa
       61 taatacttaa ggttgaaaga agaggttata tattttaagg taagtcccat cattttcaat
      121 ctcaattaat gcctgattga ttttttcaat cagttctgct tgatctaatc tcgcgacaac
      181 tccaaatcct atcccaaaag gaaaactgtc gcccaataac ttacaatccg gtgtactcga
      241 tgcccagtat tgcgcttgat cgctgtttgt cactattgcg ctaacttgcg gatcagtcaa
      301 cccattaagt aaatccataa atgtttgata atacttaaat tgaatatcat tgttaaaata
      361 ggtcgttaaa taatcggcag taggcgaacc taaatgaatt cctatagtct ttcctttgag
      421 atcgtcaatt ttttttatat tcgagttaga tggtataaga aactggtgat agctggattt
      481 gtagggaagg ctgaataaaa atcgttcgct tctttcaggc gtaatattta tgctgccaat
      541 gcctaaattg attgcaccag aattaatttc agctaaaatt tgttcaaata acaatggctt
      601 gtattggcat tttgcatgga tgcgttcgca aatctcatcc attaattcaa tttcaaaacc
      661 ataaaactca ttttccttgg aaggattgtt gacctgaatt tcaaaaggag gattataaga
      721 caaggtccct atgaccaaat caagagcata cgctccataa aagggaataa ccagtagcaa
      781 agaacataaa taaattctga ggcgaaccat ttaaatatcc tttttatata gttatttatt
      841 attttagcct tatttttgaa aaataaaaga acggttagat ttatattttt tcataaatca
      901 ggagtgttta ccttgcatag aacagagcaa ggtaaattac cagttgtacc gggtttgtta
      961 aattagtggt atcaagcaga agaattttgc cctgagaaac gataagtcac ttgaggctcc
     1021 aggtcatttt gcttctctaa ccaattgatt aattttggcc ggaataattt aaaaaacaaa
     1081 tagaaccggt tgcttgaacg aatttgtgcc atttcatccc ttgcgtacag ttctgcaacc
     1141 tcggatggtg ttgttaattt taaatttaag gctattttat ccatatggaa agcaatatcc
     1201 gtcagcgcac aatcacttaa cttgaattca ccatcaatga tttctttgcc atttaatttt
     1261 aaatctttca aagtgaattg atgttggtag aaatattcac aagtttttcc attggctgct
     1321 tttaattggg gatccgcccc agcaatctcc agctccctga ttagctcatc aaaatgataa
     1381 aaattagccc agtgcagagc ggtcatgcca tgatgatctc tttgattgac atcacagcct
     1441 aattcctgca ttttcgctaa aatcaatcgg gctgactctt tatcagcaag tttgcaagct
     1501 aataaaagtg ctgtattttc ataagagcat tttattagta aattgcgatt ggcaaaagct
     1561 aatgtccgtt ccagattccc tttatcactc tcaataacgg ccagatgcaa aggtgtgttt
     1621 gttggtactt gatcgtcata gttttcttta ctggggaggc tcattgcgca aaagggtggc
     1681 aaaatttctc tcaagaaaga cagcgcgctg tcgtgcgata gttcttcatg atagagtgaa
     1741 aaaaaagcgt caagagcatc cagttgattg aggagtggat agatgacctc tagaatttcc
     1801 tcggttaaca aatcgttaat ttgtaattta agcaataatg actcgtactt ttccatgatt
     1861 tgcccattac ataaatgggg ggtcatatta ttcataaaac tttggcttaa tatgcctagt
     1921 ttgtgcgtag tgctcataag ttttgaaagt gccctaagat cagggatacc aagattcttg
     1981 atgattaata aatgagcaat ggcaggaatg aaatcgctgg gcgttgcctg ggttaagcca
     2041 aacagttcaa aaagaagaat ccattcatcg tattgctcaa atacgccatc cccctggtaa
     2101 atttgcagag gaggcacttt cgttatgtta gtatatcgtt ccagtttgtt tttttgagcc
     2161 aaataggcta ccaactcata ggttttattg tcttcaggtg gaagcatcct gactgcttca
     2221 accaaagcag ttcttgatag agagcttgta ttttcatcaa catagatgcg gagagccaag
     2281 gtatgattgg agaaacgcct gtgtttaatg aaactgatta aatggattaa ttccgttaat
     2341 tgttgaaatt gccgttctgt caattttctc ttaaagcctt cagccgccct tgtttcaatt
     2401 tcatcgatgg gaattcccgg agtgtcaatg attgccagga accagtcgtt aaatgtgata
     2461 gtttcaatat ggggaattgt tgcggccata atcactcctt aaataaattg ttaatattat
     2521 aaacaaaaag cattattttg ataacagtga aatgacctct tgaaagtaaa tacgataaaa
     2581 gagttgaaaa agtcatagtg tcggatttca tagaatctgg tggttatagg ggaaaagcgg
     2641 gtgagcgagg ctttgcttta ataattgcag gaattaatgg ttcattttgt agatattcaa
     2701 tcttcttctg gattactatt gataagtaag gtaaatagtg tgagtagcca tcttgaaatt
     2761 tcggttccgt ttttatctgg gtattattct tttaattttg gcgatagttt gccctttgct
     2821 gatcccgtta attgtgcaat ccaacttctc tgtgctgata aagggatttc tatcttcaat
     2881 attagtattt ggtcttccgg aagttttgat tgtcttagct atcggtttac ttggaaaaga
     2941 agtgtatggc tttctcgagc aaaaaattaa aaatatcctt tttaaggaaa aagtatcaag
     3001 agcccgttac cgcctgggtt tggtattctt tgcttttcca cttgttatag ggttgctgga
     3061 ggtgtttgta aaagaaatta ccttagccta tggaagttat tattattggg tagaaattat
     3121 ttggacaacg atgtttgctt taagcttttt tatttgcggg aaacaatttt gggataaatt
     3181 taaatcttta tttatttatg attcttatgt tgtttttgaa aaaaataaaa aatgtgttta
     3241 ttaatcgtga ttgtttctag tttagtccgt cccccttata gcgttcgcta tctggcgatt
     3301 tacaatattc attgatttta ggcaagctta atttattaat atgttcttgc cccggcaata
     3361 tctctattcc ccatagtgag aatatggggt tatcatctat cgtatatctc gcatctgcga
     3421 ggattaaggg atttttattg gcaacaatta gaaatccatc ggtaaaccag tgaaatgtag
     3481 ccaaatcact tttttgtccg gatgataaag caaaaggaat tattgattca ttaaagctta
     3541 aagcctgccg aatcagtctg attgtggttg gataaaaaag agacgttttc gcttccgcga
     3601 taattaaaca atcttgttgc ttggctatga tgcgccaata agtagagctg gccaatttag
     3661 gcagggcacg aggattgttt attttgaagt tattagtcgt agcgtattgg tgaatgccat
     3721 ctacagcgcg attatgtaat atcgtattaa aaatcagaaa taaaatgcag aaaaacaggc
     3781 ttgcaattac tgccttttga ttttgataga tgactgacca ggctgttcct agcaatagag
     3841 gtaaggtaaa tacgggatca ataatagaaa taatatccca actaatacga ctatcgctcc
     3901 aaggccaaaa taaaacggtg ccataagaag tcaaggaatc caataaacaa tgagttgcat
     3961 aaccaacgag agaggctcca atggttactt tccaatattg tctatattgt gaaaaacaca
     4021 atagaaacag tgcaactatc agccctccaa ttgggataaa catcaaggaa tgcgtaaggt
     4081 aacggtgcca ttgctcaagg ctcaatggct cttggctgaa acgaataaaa acatccaaat
     4141 ccggggccat ccctgctaag gcacctgctt gccaggggat tctcttgtca tattttccta
     4201 aagccgcttg tgcacaagcg gcacctaaaa caccgtgagt tatcggatcc attttttact
     4261 taacaggctg ataatacaat gaaaatgcgc ttcttctgag aaagtcctag ctttatttca
     4321 ttgaaaaaat gagtattata tgatgcaaaa gaatttttat agatattttt taaaaaataa
     4381 aagtaatttt tttcaatttt aattgaattt aatgacatgg ttcaattttt tgtaggtcca
     4441 ggagtgtcat cgagtcgatt taatattgct tttagtaata ctgctggaaa tgtagggggc
     4501 agtgatagtt tgaatgaatg gctaactggg ataagtatta aggcgggttc aataacgaag
     4561 ctaactcaca atatagactt acttttaacc tatcaattca cacaatataa cagtattgcc
     4621 ggggttcaaa tggaaccttt atccgaagat acgttgagag ggagctataa gccaaacgta
     4681 aacacagtgc taataggttt taaagtacat ccttcagatg aaatcacaaa agacacttaa
     4741 tctcagttat ggataagacc tcaattgcag ttatttgagg tttcctatcc ttaactgatt
     4801 ttaaataatc agactgagca cgccatatcg tggccgcagc aaacgggtga tttgatttga
     4861 ccgcatccgt tggggcattt tgctacttga actttattcc catcattttt gactatggtg
     4921 ccactaacca acaatgcgtc acatttactg caggttaaat tttttacgcc cataccacat
     4981 tgattgcatt tacattccat tataatgact cccttttatt taagtagaaa aacttttatt
     5041 aattataatc catactaaat tgaattggat tcttcattag atttctgtcc tagcaagact
     5101 tcattgacac tttctctaat gctttctgtt attgaaccaa ggtttaagtg atcttctgtt
     5161 aaaccaaatg gttcttctaa atttcgagca attccctcta tggcgatgat taaataggta
     5221 ataataatgg tagcaggtat tgcccaaact ccaagcgtat cgattaggct gcaaggtaaa
     5281 aaaaggataa aaataatgaa tacatgcttt acaaaaaccc gaaatgaaag cgagataaat
     5341 gtatttttaa tgcgctcaca accgccgcaa atatccataa atttgctaaa ttcgttatcc
     5401 aataaccata gattcaatct tttcgatgct ttggtcttct cagaaagctc acgatacagc
     5461 tcttgtgcaa cataagacgg cacatgactg ggaagacttg ccatttttaa aaaattttta
     5521 aaatcatccc gagtactttt ttgcctcaaa tggatggcca atacttcaca aaacgtattg
     5581 atgagtccag agaatttttt agcctcatcg atattgggtg ataacaatac cctgattttt
     5641 attgctaaat tacgactggc attgactaat tgaccccaca gggttctcgc ttcccaccaa
     5701 cgttcataag cccgattgct tctaaaaacc aaaagcaaac ctaaaatgat gccaaataca
     5761 ctttgcgaat aggatagcca gttactggaa tgcaagttat cttgctcaaa ccaaaaataa
     5821 ataatggcgc tgtaaacacc aaccaaaatg ctaattccaa gtactttctt tagagcatgg
     5881 gtgaaatgta cttctgaaaa taaatagcta atcatcgcag tccatttgat tgttttgttt
     5941 ttccagaata atagaaaccg atctttctat aaaggccttt gccaaaaaaa cgcgagagca
     6001 aaacaagtaa taaccagaga agccagttat tacttattgt tcagtctaaa ttcttaaaga
     6061 gtaatagtct gtttgttaac agttaccacc atttatgggt gttgatgagt attgggtgct
     6121 ggttgctggt gataatgctg attctgagaa gcgtaatagg gtgagtgatg atgcgttgta
     6181 aaaaaactat tggggtggta catgggcggt tgacctggaa gataagacgg gtttggatta
     6241 acaaccacag tctgttgtcc attgctgact tggacagtag catttgatgg gtgagagtgt
     6301 gttgcagggt gatgatgtac tcgtttcata gattattatc cttataaaaa tacaactgca
     6361 tgacatacat aaaactgact ttactcaggc tatttaagaa tagcctgagt gcgagattgt
     6421 aatgtagata gttaaacaag tacagatagt attaaaaatt tactattttt tgtttttaga
     6481 tacatgcaac aatagtaaat ccaggccacc ccttattatg ttaaaagaaa tttatttccc
     6541 atgctgaaaa gaagcagccg gttgatgttg cctggatgtg tcttcgccat ggcaaagttt
     6601 ataaaaaaaa caataaagat tataataagg tgatgcacca ggaataaaat cgggggcatt
     6661 atttcttctc acttccttct tttgtgcttg ttccgaagtt ggtttatcag tctctttttt
     6721 ttgcatgtta tttgttgatt tcataaatct tatccttgat taagaaaata gattgaattt
     6781 taagtatcgt tcaaaccagg aaagagagtt ccggtttgtt tacaaatgaa ccgttttatt
     6841 gctcatggct tggtgattat agttcagaca ggtgatcaat tgtagtagtt gtcctggatg
     6901 tgttatggtt atctgactga caggaattga ggtgccagaa caaggaatag ataatgaact
     6961 gttttccttt ctttgcaaaa aaaaataaaa aaaataacga agaaaaagcg gaaaaaacat
     7021 taagcgtatt tcctgcaggt acttctataa ataaaacaac ggtaaaggaa atttgtttta
     7081 cagatatctt ttgtgacacc catccttttg tttggtatta ctatccagag cataatctgg
     7141 caatttggac ttcaagactg gcagggacaa cccaagaata tccggcagat tatttgctaa
     7201 atcaacttgc aaaaattcct ggaattacca tcgaccaagg cgatgcggcc atcaaggatg
     7261 ttagtacttt cctccaggac catcaaatga ttcctattag aaataacgaa agctggcgat
     7321 atatcaagtc ttcttctttc tcaatgagtt aatgaagaga ttgaaagcag gtccttgttc
     7381 ttaataatgg aagattttgc tggctcagtt tcgtaagtga tgcgcaaggg atattcaaat
     7441 gatttctctc atcttattga ttacatagag cccacacata atgaaacatt agaaagttat
     7501 gatcataata atatacaatg agatcattta aattcctaaa tgagcaaggc atgaaaccta
     7561 ctgataaaaa acactggtct aaaatcgaat atctacacca aagcgtaaaa aatccaaaca
     7621 tccatatcaa gggtaaacac agttattata gcgatgcctg gaccggtagt tttgaggaaa
     7681 ccgtcgttcg gtatctttat ggagatgaat acagtttaaa aacctggcaa ccacaatggc
     7741 agattgatca attatatatt ggtgattatg tttgtattgc tgctgacgtg ataattctgt
     7801 taggtggaaa ccataatcac cgagcggact ggttttgttt gtatccattt gcagataaat
     7861 acgtagaggc ttatcaaggc aaaggcgata ctattattaa ggatggtgtc tggcttggca
     7921 tgcgtgctgt tattatgcca ggcgttacta ttggcgaagg agcaatagtg gctgcaagca
     7981 gtattgtaac caaagatgtg gagccctaca gcattgttgc tggaaatcct gcgaaaccag
     8041 tcaagaaaag atttgcagaa aacatgattg aacgaattct ggccttgggt atttattcct
     8101 ggagcgaagc caaatttaac ttattaaaag actatatttg cagcaataac atagataaat
     8161 tagagcaggc cagcaggagc tatgatcaaa accataccat agaaagcaaa tgataaatat
     8221 caggaaatta agattaggtg atgaatccac cttggaaatt tttttaaaat ctcatgctga
     8281 tgagagtatg tttttacgca gcaatctcta tcattccggg ttgtcttatc atgataagcc
     8341 ttttcatggg gattattttg ctgcatttga taacaacaaa aagataagtg gtgtattagc
     8401 ccattattgg aacggcaatg tcattgtgca gtgccctgat ttcaatgttt taacaacact
     8461 agtgggcact tttcaaaaag ccgctacccg agaaattgca ggtattctcg gcgaagatca
     8521 gcaagccgat gttgtcatga aacaactcca gctggagcat gtagattatg ctgtgaatta
     8581 cccggatggg ctatacgcac ttaatttaca gaatctggca atgcctggta atcaagacag
     8641 atatcaactg aaagtcgcca aagactgtga taactgttta ttatatgaat ggcttagagc
     8701 ttatcaaatc gaagccttag gtgctgaaga agacagttcg attttaaaag agcaagtaga
     8761 gagtgaagtg aaaagtactc tcgaaggcaa tgatcgttgg gttttagagg agaacggcat
     8821 ccctgtctcg ctatgcggtt ttaacgcaag attaccagat attgtccaaa ttggaccggt
     8881 atggacacca gttacgttgc gggggcgagg atatgctcga acagttgttg cgcttagtct
     8941 tagtgcagcc caaaaaagtg gagtgagtcg agcggtatta tttactaata atcaagctgc
     9001 cgtacgagct taccaggcaa ttggttttaa taaagtgggc aattatcgat tggccatatt
     9061 aaaaaaacca atactcttga cacgttaatt gtgaatacct gttaaaacag taaaagtatt
     9121 tattgatagg aaataaagcg atggatatgg atattaaaca taagcctttg aagcgtgaac
     9181 tcagtctatt tggcgctacg atgatggggc tcggctctat tgtcgggacg ggtgtttttg
     9241 tcagcatcgg aattgcggcg ggtgtcacag gaccatccgt cattcttgct attgctttgg
     9301 cagcacttgt tgctacctgt aatgcattct ccagcgctca acttgctgca aatcatgctg
     9361 ttagtggagg tacttatgaa tatggatatc gttatttaca tcctgctttt ggttttactg
     9421 ccggttggat gtttttatgt gcgaagagcg cttccgctgc aacggcagcg cttggctttg
     9481 ccggttattt tattcacttg tcaggtttag agacggtgcc tgtcatacct attgctttag
     9541 cagcgtctat cacgctaata cttttagtct taagtggact cagaagaaca aacacagtga
     9601 atattattat tgtaatggtt acattaataa ccttaaccgt ttttataata agcggattac
     9661 ctgatttcat gcaaagggga actgctaatc tgaggccctt ctttcctgtt tcaaaacaag
     9721 aaggactaag tcattttttt tatgccactg ctttaatgtt tgtggcttat accggttatg
     9781 ggagaattgc aacattagcg gaagaggtta aaaatccggg aaaatttatt cctgcagcga
     9841 ttattatcac tctgatggtg agtgccgtac tttatatgct ggtggggctt gtcgctattg
     9901 gagctgtagg ggttgaaaaa ctggcccaag tgaccgaaac aaacgcaact cctttagaga
     9961 gcgcggcacg agctattggt atccccggat tgagtgttct ggtctccatt ggggcatgca
    10021 cggccatgtt aggggtatta ctcaatttaa tacttgggtt atctcgtgtg gctttagcta
    10081 tgggtagaaa aggagattta ccgaagatgt tttgctatgt atcagaaaca agacatgttc
    10141 ctgtttttgc tgtcattgga gttggagttg taattattgc tctggtattg actggaagtg
    10201 ttgaaacaac atggactttc agtgcgttta cagttttaat ctattacgca attactaatc
    10261 tcgctgcctt gcgtttgcct aaagagaagc aattgtatcc acctctgttt gctatcggag
    10321 gcttactgtc ctgccttttc ctggcgtttt gggttccaat tggagtatgg ataacgggaa
    10381 tcagtttact tgtcctgggg cttctttgga aagttgttgc caatcgtttt ttataaaggc
    10441 aagaaaaaaa gacggatata ttctagaata aacaagaaga tcagagaaaa ggaagaacaa
    10501 tgatcgatgg aattatggtt ttatggttta ttttactgat gctctctttg gcttttgttg
    10561 cttatgatgt gagggagatg ccaattgatt gggtgcaaaa actaggatgg gttttggtcg
    10621 tggcctatac cggcccttta ggcttatttt tttatttttt gacttgcaga agcccaggta
    10681 aaggattgca tgcgttatat actaaagcgc aatggaaaca ggctgttaat tccgaagtgc
    10741 attgtctggc tggtgatgcg acagggatca tcttcgcggc aattttttta tcttttattg
    10801 atttaagtaa tggtatagag ctgattctcg agtatatttc agcctttttc tttggttgga
    10861 ttatttttca ggctggaatg atgagaagta tgtattcaag ctatggagaa gctttaagga
    10921 aaacattttt tgcagaaacc ctatctatga attttgtgat gatgggtatg attcctgtga
    10981 tgatgatttt aatgaacaca ttagactatg gacgtaatcc agcgcatttg caattctggt
    11041 ttattatggg aatggctact gtagtcggtg gttttctggc ttttccaatt aataactggt
    11101 tggttaagaa aaaattaaag catggatgta tgactttgcc agagggggat gaacaacaca
    11161 tcgaagggca tacgacccat catcatggag gtcatgaaga gcatctacag gcacaagcta
    11221 aaagaattgg ttcgaaagtc gttcatggac acacccacga aatgggaagc ttaccattaa
    11281 acattcaatt gaaatatatc ggtataagtt ttggagtttt attgattatt gttttgataa
    11341 tggccttttt tgttccattg aaaatataac agagattttt attgaatctg aacaatgttg
    11401 gattaaaata atccaacatt gttttcgtct tattgcatgg ttcttgatat tgcttttcca
    11461 gttgctttaa tatcttgttt agctcctgtt acagtcccct tgacagtatg gcatgctgct
    11521 aatagagcaa tgctgatact gagagtccct aacttgatta aattcctttt taagttaaac
    11581 ataactcctc ctttatactt atgattcact tgaattttaa acgcccgttt tttacttgct
    11641 gctgtatgtc ttgtacaaat ggagacacgc aggttgttaa acgagccata taaaaatcaa
    11701 ataattacgt tggtattttt agttaatttt cataaaaaga attctttaca taaagaatag
    11761 aagagtattc ttaattttca agatacaatt tctatccaat gaaacagcca ggatttacga
    11821 aaaaaagtaa tcgccagaat tcgttcttgt tacaaaatta tttgtaggta ataaaactga
    11881 tacgccccgt tcgctccaaa cgagcttctt tcactgttgt caaatcactt gcatttaatt
    11941 tttctctgaa aacttgcctc aaatcttctt cagtcacttg atgtcttttc aaagcactcc
    12001 aaatgagctg cccatcttgt attaatagca ctgatttccc tttaaccagt tttccaaatc
    12061 cgtgggaata aaaggaacaa atagcaaaga tccaatgtaa aaaaataagt aaactgctcc
    12121 cagccattgc agatattaaa gtagagttgc cattaattgc acgacttaac acggcgccaa
    12181 tgacaaatac aaaaatataa tcaaatgctg tttcaaaatt gtatcgggta ttgccaacac
    12241 ggatgataaa aattgcgaaa acatagataa taaaagttct tataaaaaga tataaaaggt
    12301 ttggatcgga attaatctta tggaggtagt caagccacat atctttttca acaaatcatt
    12361 tatcctaaaa ctataaatag aattgctttt cttttgcaat aactttatca aattttaaat
    12421 ggattgcttt tgaatgcctg tctggataga gagttaatct gttgtttata aacatcactt
    12481 tcggcagata atcctctatc caggctaaaa ccttattttc tcagattagt tctgataatt
    12541 aattgatatt cacgtcgttt tttctttcat aaagagttca ttaagcttgt cttcgtaaaa
    12601 atggtaattt aatacctgat acaactcttc gcgagtttgc atctctgcca gaatatcgtt
    12661 ttggctgccg tttttgcgga ttgtgtcgta aactttttct gcagcggcac tcattgcccg
    12721 aaatgcactt aaaggataaa gagccaggct aactcctact tcttttaatt cttccagcgt
    12781 aaataatgga gtgactccaa attcagtgat attcgccaat accggaacat tacattgctc
    12841 agttagcgcc tgatattcct caagtttacg tactccttcg aagaaaatca tatctgcccc
    12901 ggcttcaata taagcactaa ttcgctccag cgccttattt aatccttcat tcgccaggga
    12961 atcagttctt gccataataa caaaatcagg atcggttttc gcatccacag cggccttaat
    13021 acgatctatc atttcttctt tttcaaccaa tgccttaccc gggcgatgac cacatcgttt
    13081 cgcttgaacc tgatcttcaa tatgtaccgc tgctgccccg gctttaatca tttctttgat
    13141 agtccgtgcg atgctaaagg ccccacccca tcctgtatca atatcgacca ggaggggcaa
    13201 atcaacggca ctcataatgc gccgtacgtc ttcgagtaca tcatttaatg tggtaatgcc
    13261 caggtctggt aagccgtgag aggcgttagc tactcctgct cctgaaagat aaattgcatg
    13321 aaatcctgct cttttggctt gcaaagcggt ataggcatta attgttccta caacttgcaa
    13381 tggtttttct ttcattaaag cttcacgtag tatttttccc tggctgtatc gaaaatttga
    13441 catttggttt ccttgttttg cctaagatga gaatttatca ggacttacga aaaatcccaa
    13501 tctctttgtt aaaaaaagtg tttaattcgg tcatttagtt tatctaaact ccttcattaa
    13561 aaacattttt tgccttgatc ttggggtttt tcgtaagtct ggtttatttt atttttacgt
    13621 ttttttggat attttttcaa acttctggaa atgcacagca aaacgacagc caaagcttga
    13681 aaagcgtata gatcatgtac caaagacaag cttgtttgcc tgatatcata acgagagaag
    13741 gcaaaaatca attacaaatc atgaataacc cagacatttg gctcaatgta tattccaata
    13801 tccttttctt tattgatagt gacaggattt aacccgcctt taacaagata ttgacccgtt
    13861 tgaagtaacg gatggccgaa ccattgttcc cactgagcaa cattcgctgt gatcacgcgt
    13921 gatcgctggc atggataagc tatttttcct cccagttgtg catgaactct taaccaagga
    13981 tcaaaaatag tattatccac tcttttccac ttcaaataat cctctaggga aataagcggg
    14041 tagttttctt tttggctagg cctggctggc actattagtg ctttatattg agcctcctgt
    14101 gcactggctt ttaaacgatt caataagagt ttgcttaaac caagtttttg gtaagccgga
    14161 ttcactgtca ctgatacagc agcaagataa tttgattggg ttaagggttt tgtagattcc
    14221 aggtgagaca ggacccaatt taatccttct tgtggcaaat tatcgagtga ttgatcaagt
    14281 gctactggca aagaattaat aacagctact acctcaccat tatagtgaga tagccattgg
    14341 tataaaggat aatgactata gagcttaaaa agagtgtcat ttttaatgcg ttgataatca
    14401 ggccaaatct tttgtcgtaa atgtagaatt ttctctatcc agtcttcttg cgccttgcca
    14461 gttagccaca taagtttatt gagcatgagc ttgttccaaa aggtgtgctt ttgcgttaat
    14521 taaaccggac caccaatctt ccattatatc gatcgccgca tacgcgccat ccattatttc
    14581 tttttcctgg agaaaaatat tgggatcaac gtgctcttca gcaagtatat gatccataaa
    14641 tttgcctaac tcatctttat gtgcctgttc tactccatcc aggtgtaaat catggaattg
    14701 cttgatgcga gaattctcaa aatccaaacg ttttctttga caaagctcgg cgcttatttc
    14761 tttaaataaa atatgctcaa atattgcagc tgtttcagta gcgtacataa cgccaagggt
    14821 tttggatgcg ttaatctcaa tcaaggcttc aaggcgcata aaaaactgtc tggttgccac
    14881 acatggaatc ctgtttgtta catccaggcc gatttttttt agtgcatgcc gatacatttg
    14941 agcatgattt ttttcttcgt tatagttagc gagtaactct tcagaaactc cgggatcaga
    15001 aagtgacttg gtttggagat gagaattcat taagaaacgg gtattgttta acgaaaattg
    15061 ataatgctcc atggttatcc attcaagata actcgtcgaa aattttttta ggtcactaaa
    15121 ataatttaaa atttcggtag taaattggtt gtttctctta tccatgtatt ctcttatcat
    15181 gaagcctcca gtcagtatta aaatcaattc atcaagggtt taccataact ataaagatca
    15241 tataattaaa atattatttt ataattatct tgataatttt tagttatgat aaatttttca
    15301 caaatccagg tatttattac aattgtcgaa tctggtggat ttattgctgc tggagagaag
    15361 atgcatcgtt cacagccggc aatcactaat gccataaaaa aattggagtc cagcctgggc
    15421 ttcgaactgt ttgatagaaa aatataccga cctcaactca ctgcgaaagg taaagctttt
    15481 tatcaaaaag caaaagaagt ggtcgctcaa tttatgcaac tggaaaatag tgctgaaaca
    15541 ttgagatccg gagtagaaac acaattttct atagatctcg atattgcttt accattgccg
    15601 ggatatgctc cgcttattca atctttcata agacaatatc ccgaaacttg tttttcacta
    15661 agtaacaata cttttgttcg atgtcttgag cgcctggaaa gaagagagtg ccatttatgt
    15721 atagctgcaa gccatattcg ttctccacag atagaatatt tgcgtatgcc ttccatacga
    15781 atgattcctg ttgcgagcag ggactattat gaccagcata aagaacgaat aaataatccc
    15841 aagctgggaa tatactgtat gcaaattatg atagatggca cacaacagga attatatcaa
    15901 ctcaagattg agccattaag tcattctatg cccaaatggt tagtgggtga tatgtatatt
    15961 cgtaaacaac tgattttatc gggtatgggt attggccgtt taccggatca tctggttgaa
    16021 aaggagttag cggagggctc tttggttcag ttaaatacca gggattatgt agctgttact
    16081 atggatattt atgctatgcg gttaaataat caagagcatg gtatggtctc caattatgct
    16141 tggcagtcac taaaaagtta tgctgaagac acagtatctc ccgatttgaa ataaccaatt
    16201 tttgcgtttg cttactaatt caagcagttt ggtggaccga atatttgaaa aacaggaggt
    16261 gattcattga cataaaacca agataagcct aaatttaaat caaaaagaca taaaattgat
    16321 ctccagttgg atattataat gattgatttt aaaataaatt cccaacctga tgatgaatct
    16381 tgcgggccaa cctgtttaca tgccatatat caatattatg gcttggatat cagttatgaa
    16441 gaggtggcaa accaggtaga gagcttttta tctggtggaa caatagcgcc catgcttggt
    16501 aaacatgcct tgcaattggg atttaaagtg accctttatg tcaataattt acaattgtat
    16561 gacccgacat ggtttaatag cgatggttca tcaaataccg acgttctgag agaaatgctc
    16621 atgcaacaaa tgaggtataa gcgtaaccag tatcttgccc gcggaacaca tgcagcactt
    16681 gattttctga ctttaggggg cgctatctat tttcgatgcc tgactgcaga aattttaaag
    16741 gaatattttg ccaggcagat tcccatattg acaggtctta gttcaaccta tctgtataac
    16801 tgcagtcggg aattattcaa caaggaaggc aaatcaagga gtgatcctat tcgcggccta
    16861 ccgtgcggac attttgttgt tctatgcgga tacaaccaaa aacatcgctt agtagtggtc
    16921 gctgatcctt acaaaaagaa tccggtttca ggggataatt attacaccgt atccatccat
    16981 agattaatta atgcaatcat gttgggagta gtgacgcgtg atggtaattt attgattatt
    17041 cagccgaaat acgcgtaggc gagcagcgaa ctctaactat cccagatcaa gggattatgt
    17101 caccagattt ttaatattga tttcaatata agaggccatg tatgtctctt ctttattgtc
    17161 tgctaataat tgatatagaa tggatgctgg ttaatttgat gtgtcatttg tcatgagcgt
    17221 aataattgat tccttaaaaa attctgatgt gcctcatctc tatttgttaa aggtcggctt
    17281 aacaagaaaa gaatataaca acacctccat gatgagccga gatgaaaaac gacaactggt
    17341 gaataacatc atagcgaaag cttctcacga agaaattttg aaaattatca atgacttaat
    17401 ggccattgaa ctttcaatag aatcaactga cccaattcgt acaggtaatc gcctcatagg
    17461 gcaactgctg ctgggttata tcacaaaaat tgatcagcaa aattttatta atttttatga
    17521 tcaaacaata aaaaacggca ataaaacatt aggggattat ttaatcccgg aacaagtaaa
    17581 acaaatttgg gctactataa aacaaacggc tgtaaaatat ttttctctga atcatagaga
    17641 cgctgattat caagcgtttc taaataaagg gtttagaatt ttgcctattt tttactatca
    17701 gcaacaattt cctgaaatta ctcccgagca atatagacaa ggagtcaggc cagttgaatt
    17761 gacaagagaa cgagaggaaa tcaagaacgc atttcacaat aatttgtccg ccaatgtaac
    17821 aatcccggca tttcctgagg cgaattatct gaagaccaga ttggctgaaa taaagatgca
    17881 tattatggcc aatgaatgga aactagctaa ttattcattt tatagtgatg gtgttatgca
    17941 tggtgataaa cgccttcctc atcgagtaaa ggatattctg gatgtaattg aaaaattcga
    18001 aagcagtaaa ttaaacgcca aagcggctta taagcaaatt gttgtgaaag ctaaagaggc
    18061 tttggattac ccgaggagcg gtcgattttc tgaaacgacc gatttttatc aagatatata
    18121 tagtcatcat atcttgcgag atgattacca atttaatcat tctagagaat taacaagtta
    18181 ccatggatct ttatttaata taaatcgcta aagataaatt tagtcatttt aaatcatcaa
    18241 ttccaaggca agaaaactgc tttattaact tggtagacgg cagtgcattt tgagcgattg
    18301 cccgagcatg ttattcatgt aaaaaagatg aattagagat atgcatttgc aaatgccact
    18361 agaaaagaag agtgtcctgg aagttaaagt atagatttga gtttcactac agcaggcaag
    18421 taacctggtg tgggggcgta gcctgcatcc tgaggttaca ttgtattctc tgaagtcaag
    18481 aactgattaa cccaataagg cattggtatg ctgacacagc taaagaaagg ctttattcca
    18541 gttttcatcg ttatgtacac tctgggttat tgaatgagaa tgggggaggt ggggagtgaa
    18601 ttagtggtaa aggtaacccc aggacacggg ttttaccctc ctcccaggct gctaaagtct
    18661 tgccgcacaa tattcaaaat tttcaaccca attatcatta tagctttgct ttcattcaag
    18721 ccagaatttg atttgttcaa aatcgacgaa ctatacttaa actgacatgt tctttgtatt
    18781 atcaatttga gtcggtaatg accagaagta aagggctaaa gacgcgaagt agttcattct
    18841 ttcagaatca tttgaaggtc tctgttggga aattattgtt ctcagaggga gtgggcgatt
    18901 ggtataaaaa aaagggctat ggagctcaag atacggataa ggatttcttg gcaccatttg
    18961 ctgattttct taatcaacaa aaaaacaatc ctgaagccac ttattttaat gcgttccaga
    19021 gtcttgaatt aacattaaac agggtaccct ctcaattagt ttcaggagat tggtgtgaat
    19081 tggaggtgct gaaatgcgaa ccagaagcag attctcctca aaaaccgggt actggtaaac
    19141 atattgtata ttttcctggt gcaaatacct actatcaagc ttgctttcga gatattagcg
    19201 ctgcagcaaa agaaacggga gcaacggtac acgcgctcaa ttacccaggt attgggatga
    19261 gtactggaga ggttgtggaa gctaatgatt taaccaacgc cggtattgca gtggtccagt
    19321 ctttactaaa gcagggtgtc aagccagatg atattgtttt gcaaggcgat tgctttgggg
    19381 cgtccgttgc actggaggta aaaaaagaga ttgagaagca agcggatata aaagtacgat
    19441 tgataatgaa taatgcattt aaatcattta aagcagctgt ttctgactta attggcagat
    19501 ctcctttaat tccctcgata tcaaaatcga ttgttaaaaa tttgctggaa tttaccggat
    19561 ggaatgttgt ccctggacaa aaatacatcc attcagatcc ctaccagtgt tatattcagc
    19621 atgaagggga tcaaacctta actaccgcga ctttatcctc caaggtcgct aaatacaagg
    19681 aagaaataag aacagggaga accacatccc actcaagaga tcctgttatt gatagatgcc
    19741 ctgaggaata caaaccatat cgtgactttc ttgatgagag ttgttttgtc agagttagag
    19801 attctgcaaa agaacggtta gctgctaaat ttggtgtgga cagccgtggt gaggttaatg
    19861 cccattttgc cgatctttgt gaattggaaa tgctgggagg gcaatcggtc tatgaaggat
    19921 ttgttaataa ctatttagtc atgtcagata aatacataaa agcacaccct caaaaggatt
    19981 atgatcctga taaaattaat tttctttctt ctaatcctat gggctatact ccaaaataaa
    20041 gttaattcaa agcaagtagc cctcctccca tgctctgcta ttgttatgtc gatgaggtaa
    20101 gctcattgat aaaatccggt taaaggataa tatcatccaa acattccaca atttgggaac
    20161 agtattttct cttccagttc ggtttcaacc cgatgaatta gggctgatgc agcttctttg
    20221 gtatgggtat ttaggtgttg atcgggtttg gacgtttgat ttgccagaaa agtgagttgc
    20281 aatcattgct gagcttaata actagttatc aacaatttga ttgctttcta tttttatggg
    20341 tttaactttt ctcaaaggtc ataagaatgg ggcaaggcga agagctggtt tctatacatt
    20401 ttgttactaa gagtgaaagt ctgttccgag cttcggttaa ttcattaatt tttaaattta
    20461 gtttttcaat acgcctttca gccatatctc ttacgcgctc tcgatcattt ttagcatcaa
    20521 actccaatag ttcctttatt tcttttaaag taaagcccgc ttttttggct aaaaggataa
    20581 actttaagcg acttatatca tcgtcaccat aacgccttat tttttcagct ccaagttctt
    20641 tgttaagcaa cggaacatga agcagtccct ggcgttgata ataacgcacg gtttcaatgc
    20701 caactccaca taatttagca aattcaccaa tttttttatt attcattact tgactccgta
    20761 ctatagtacg tactctatat tactatttat agaccattaa tcatagggga tagaaatgaa
    20821 tcatacttca attaaaaaag cggttttgta tcgcatggtg atagaacatc atgtttgccc
    20881 ttatggtctc aaagctagag atttattaag acgtcaaggc tatgaggtag aagataattg
    20941 gctaacaacc cgtgaacagg tcgaggcgtt taaggataaa cacaatgtga aaaccacacc
    21001 tcagactttt atcaacgatg agcgtatagg tggttatgac gatttacggc gttactttgg
    21061 aaagaaagta agagacccaa aagaaaaaag ttatcgccct gtcatcgtac tgtttgcgat
    21121 taccgcttta atggcgatgg cagtgagtta tactcgcttt gccaatcctt ttacaccaca
    21181 tgctttaagc tggtttattg catttagcat ggcagttctt gctttattaa agctccagaa
    21241 tatcgaaagc ttctcaacca tgtttttaaa ctatgacctc ctggccaaac gctgggttcc
    21301 ttacagctat atttacccat ttgctgaagg tttagcgggg atattaatga ttgcaggagc
    21361 gttaaattgg ctttctattc caattgcctt atttattgga actgtaggtg ctctttcggt
    21421 ttttaaagct gtttatatcg acaagcgtga gcttaaatgt gcctgtgtgg gaggtgatag
    21481 taacgtacct ttaggattta tttctctcac tgaaaattta atgatgatag gaatggcttt
    21541 attcatgttg cttatgtaat tcagctagca agcgtagccc ggattaagtg gaacgaaatc
    21601 cgggagtatg actgcgcttg catcagtgct acgccagcta tacactgtca acatataaaa
    21661 agttgatcat cgggctattg atatctaaga ttgttaaagt tctcttaaga cagaccttgt
    21721 ataatgagca ttttttatat ttattccagt tttgccagat gagcaaagaa atcaagaaaa
    21781 acctacagaa caataattca gcctttgtct acttgcgtag cacggggctc gctacaaact
    21841 ctctgtcgag cattctgcag tacatgacac gcatcattgt acttacggaa agcgcgggca
    21901 ttgccctggg cattctggga gtgctctttt cagtcattgg ttttgtaagc agtaccattg
    21961 caagaatact tgatccaaca gcctctaaat taagtcgtgt gatgtcggct ggtattctca
    22021 ttgcagctgc agcaggggct ataccactca tctatctggg aacgttattc cccacgatgt
    22081 gggtatatct tggtataggc attatggggt ttggcatact taattcgtca tactaccttt
    22141 attcggacgt gcgtaaggaa aatgcactca taaatcagta taaagataac cctgaacaat
    22201 atgtggaaag cattaaagaa aagcgacaag ccaagtacga aaaacttctc aacacgcggc
    22261 tcgacgataa gcaagcacga gtgctgctag tttttaagct ttaccaactt gacaagaaat
    22321 tagcctttgc taaacaagag gggccagcta gactttacga catcgtatgc catcgcaagc
    22381 acacccgtag tctggagaga tttattgacc ttggcacttc tcttacaagc cttattgttg
    22441 gggtgtcagg cctgttttgg ggaatcgctg cctggccaat tggccccata ttgctcggta
    22501 tatctttggc tgtattttct gtaagcctat caaagtcggc gatacagttt tatagaaaag
    22561 ttaataaaaa caatgcgctt ttaaaagagg ttctctcaaa tgatagatat gtatcactta
    22621 ttgatgcgaa tctggacaac ccgtatgaca tcgaagagat ggacactgtg gaaaaaacac
    22681 aagagaaggt ttgtacgcgt gagctttcac aagagtctgc gctctcgaaa gaccaagaac
    22741 ataaagagca ttataccaaa ctcttccagc ctgtgaataa aaccgctctc aggccaaacg
    22801 atgaagagac cgatgagcta agctgcaagc cggttctact ccaaaggtag tggcactata
    22861 atctggttat agcaatctaa ctccttgact ccgcaatcaa aagaggatta atgagtatat
    22921 ttttccaggc acaagagcag caggtcatat cattgaacca cgtaagcaaa tggccaatgt
    22981 aaccaaactc tcaggtattc attttacggt tcatgattta agacgaactc ttatcactat
    23041 tgccgagggg ttagatatct cggcttatgc tttaaagcgt ttaagaaaac ccatgtaaca
    23101 aattactgat tattttatga agatgggtat tcaaccttcg gcaaccctgt tagaaatcca
    23161 accgtaacag ggagtaaaat gtgaaaaatg gaatcaaaat tcataacgat ttatttgagt
    23221 aaggaacatt tagttactat gtggatatca ttcgctcctt aaaagctctc atccctttga
    23281 agattggatc tgcaatcttt tctgggaaat ggtttggtaa ttgtgttgag acctcatcga
    23341 tagcagaatc aatacggtca atcacttgtt caaaaatctg agctgcttta attttagaat
    23401 attgagcgta ttttgctgtt tcaataaagt gtcttttttg aataccgtca atcttgtaat
    23461 gactattttt accatataaa gccatcgcca ttttcaattt cttaaaggat agctgttttt
    23521 gttccacgat aggataggct gaaatgatgt cgtataaggg ggtgagctgg taaccgcctt
    23581 gtggttgtat acttatactg aaatttttgg catgcgcatc aatagcacca aggcaccagt
    23641 aaacaacttg tgctctgaaa aattcatctc tatctttttg aggctgctgt gagccattaa
    23701 gtagctgcat aatttcgggg ccaccatctg actgatattt taaatttggt gaataaccca
    23761 acgcttggca taggtcttct tgcggtaacc tgagtatata acggttatct cgactccatt
    23821 ttctatcaaa tctttcgaca accagcacct tttcatcttc gaattgtaag atttttgatt
    23881 ttgcaacctc taaaccaaat ttgctggcta gcagtaaaca gagatgctca ttttcacaac
    23941 tctcacttaa agcaattcct tgatgtggta aatatccaat aggtaatttg aatatgtgag
    24001 ttgttggtgt aggtccaaat ggtttatgcc attcattttg gtaatataga aaagccgttt
    24061 tttcctgagc accggctatt gagattctaa actcatcatc ctctttattc atccccagtg
    24121 gtgaggtttg gtaagaacgt aaagtacgag ttatctcggc ctcactgaca acgtgacttt
    24181 caatgcttag acgctgtgtc tgaggaatac gttcaacaat ttgtattgca ccaacacaat
    24241 cactgccaat taacgccaat aaatcaaacg atcgcgatga ttttgcctga aaacaggttt
    24301 gaattctggc tcttatctgt ggattatcgg gaagtaaatt atcaaaaaag ttataaacaa
    24361 cttctcctga aaacctttga tttattaacg gtaacgataa agaaactgag cgtgaatgtg
    24421 gcttggaaat ctagtcagca acgtaggaaa aactcaagcc tcctgcatta tcctgggtaa
    24481 gctcccccag aagctcattg ttcatcaata tgtataattt acgcattacc attctatctc
    24541 ttcatgagtt ttctgccctt tttcaacaag actaatgttt aaatttaggg cggacaatat
    24601 cctaaacagg gtatcaattc gactattgtc gggactattt tcaaattttg atatggcatc
    24661 ctgttttaaa ccgactaaat cggtaacttc agattgactc attttgagtt gcttgcgcct
    24721 gtcattcaaa tataatgcca aatcttttgc tgagctaatg atcatcgact tcctcttttt
    24781 acccccaatc gccaataata ttaactttac ccgcatttga gggtaaaaca ggattttatc
    24841 atacagatag ccaagggttt cgaatctaca tggtgttaac tgaagcctac aaaaatgaac
    24901 tttgcaaagg cgttgaacaa cgcaccgtaa tcagagtttt attacaagct ggatggctga
    24961 aacccgcccc ggatggaaaa gcctcccaca agccaagaat caaaggggta ggtacaccaa
    25021 ggctgtatgt ctttaccggt aaaatttggg gaggtgaata acaaattgat tatacaaatg
    25081 ctttttacgt tgagcaccga aggtgtgaat aagattcgtg aagtttggta acctgcttgc
    25141 tggttagcta acttcaccta tcttgccctg tattttgaaa tttatttagg taccacaatt
    25201 tggtaattat tcgtaattgt tcgttatata acgattaatg acgaaaataa tcatgaataa
    25261 acggaaataa ataataaatt accgaaaatt accaaaaaag gctttgccag ctactgaaaa
    25321 ggtcgaaaat aaatagaaat aaacgataaa actcaaagga ttgaacaacc atttttacgg
    25381 agaactgata atgaaaaaat ccttaataga aacagtcaca actcgtaaac aaagcccaaa
    25441 aagaacctca gcaagaaaaa gcaaagtgga atttctagcc ttgcgagaag aaatctcaga
    25501 agccttggaa aaggggtggt cgcttacagc catatgggaa actttgtgcg atgaaggtag
    25561 catcaccgcc acctataaca cattccgttt atatgtattg aaatatctga acggccaatt
    25621 gcccggttac aaccagaagg acagcattga ctaacgccat aagaaacaag tgaacaaacc
    25681 taagtcgaag aaaaaaatcg atccaatacc atcctttaca tacaatccta tccccgatat
    25741 cgaaaaactt ctgtgaaaac aaggtatttt aaacatgcgc tgaaatatcc caagtcaaat
    25801 tgtccctttt gccccatctt gaattttaag gtggggatgc ctctaagcca gcaataatgc
    25861 ggttgaccca acagtcccat ttgtccctgt agtttttgat aacatagtaa tgctattaat
    25921 tatttttaga gataaaccgg gttacactat ttttgttcaa tggcattgag ctcttctgag
    25981 caaatggtcg gaacattttc atctttataa gataaaatca agattggtat tcagtgtcaa
    26041 ggcaggaagt gcaatgacgc tgaaaatata attaaactat aattggaaaa ataatacatg
    26101 gaagaggtat aaaaatgttt cagattagta aaaaatataa tcaagataga attaccattt
    26161 ctgaagaaga attttctata ttaaaagaaa cgagcgacaa aattcgccta gcactgtcat
    26221 ttgaaagaaa ctttgaatat ctgattcaaa actatctgga ttatgaaaac acaatagctc
    26281 aaaagtctat tggtcaattg gttgataacc ttcaatcaat agaggaattt aacagccata
    26341 taaatgatgt taatttaaag cttctaaatc tattatctac cactaaagct tatattgata
    26401 agagtccatc aattctgaaa aaactcacgg gtgaggcaga ttcatttaaa attcaatgct
    26461 cattgcagta cgacacaaat tttagttatc ggtttgttga ttcattgaga aattatgccc
    26521 agcataataa tgatccctta tcttcattga ttttgggagg tgaatgggtg gagcgtgaaa
    26581 atcttgaata ctatacaaat cccattattg ataccgaggt attaagagct aataaaaaat
    26641 ttagaaaaag cgttttgcca gaatatgatg ttaaatttc
//
LOCUS       DBNCWK010000026        26507 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.026, whole genome shotgun sequence.
ACCESSION   DBNCWK010000026 DBNCWK010000000
VERSION     DBNCWK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26507
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.026"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(33..755)
                     /locus_tag="AC06IO_001043"
     CDS             complement(33..755)
                     /locus_tag="AC06IO_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9874246.1"
                     /translation="MMQRKWVVLGATSVIAEQFAHFAAQAGNHLCLVARNSEQLALIS
                     KDIQLRYKIPCNAITINLSEPNEQLLTILETRERELDLFIAHCDFTDNNHLNPQSIKH
                     LIDTNISSTAILINTYLQSQQEKHRIVYLSSVASCRGRAKNSLYGASKAAIEIFLEGL
                     QQKATPHQHILIARLGYIDTKQTYGLPGVFYAAQPHDCAKACWEKINHNKRLFYYPSF
                     WKVVMAIITKLPFFIYKRMGKI"
     gene            complement(752..2047)
                     /locus_tag="AC06IO_001044"
     CDS             complement(752..2047)
                     /locus_tag="AC06IO_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIO9874247.1"
                     /translation="MRSKRILLTNFTRAIYSESCCIRPDTEKELIHYIAQNQPKNMLT
                     RGAGLSYSDSCLNQDGLVIDTSRFNHFIDFDVETGIVVCQGGTPFKDLFLLHDEFIPP
                     VLPGTAFATVAGGIAHDVHGKNNHSAGSFGHHISWFDLLIGDQIMHCSREKNSDLFFA
                     TIAGLGLTGIITQVAIRLKKAPRHLAIKNKPFASIQELIAEMATYGLQQDYQVAWLDL
                     LYSEPRAILSTANYCESPNYPDKKLKIYSLPKLPFGLIKKWNMKVFNQYFYSRQKENE
                     MMSLTQYNNPLDKISHWNRLYGPRGLIQFQAVFDQDKAAQIINHILEIIRTHSATPTL
                     TVLKLFTQPGLGLLSFCRPGFTLAIDFINNPHAKKAISAMNQFIAENDGCVYLAKDLL
                     LNPEQYFTMYENQSKFAQILTKYRCPMRSDLATRLGIIK"
     gene            2166..2405
                     /locus_tag="AC06IO_001045"
     CDS             2166..2405
                     /locus_tag="AC06IO_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874248.1"
                     /translation="MNKIFNLASIACFLFFSLIAFAADIPGLDEGTDKLNMEECVGEN
                     YQQCLDDVCLTSDEIDCNSNCQSLAEDKCEQQAVD"
     gene            2850..3911
                     /gene="fliM"
                     /locus_tag="AC06IO_001046"
     CDS             2850..3911
                     /gene="fliM"
                     /locus_tag="AC06IO_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HIO9874249.1"
                     /translation="MTEKDVLSQEEIDALLDSVDESIDDETNDEELSSRDQPVRKKPE
                     DTTSQELNSNFDSDKKVAEEGVKTLNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYH
                     LTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDY
                     YFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLIHACEPIIQLDVTKFNDETNPQ
                     LVNIAEPEEMLLVARFVLNFGKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNW
                     INSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEINEEVTLDIEGTPSFTA
                     TLGSANEKRALKVIKIIRY"
     gene            3921..4250
                     /gene="fliN"
                     /locus_tag="AC06IO_001047"
     CDS             3921..4250
                     /gene="fliN"
                     /locus_tag="AC06IO_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HIO9874250.1"
                     /translation="MTQVNETSTEQLNQGNPLNPDPGNEKLELILDIPVSVTVEIGRT
                     KMTIRNLLQLNQGGIVALDRLAGDPLDVLVNGTLVAHGEVVVVNDKFGVRLTDIVSKA
                     ERIKRLK"
     gene            4247..4657
                     /gene="fliO"
                     /locus_tag="AC06IO_001048"
     CDS             4247..4657
                     /gene="fliO"
                     /locus_tag="AC06IO_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HIO9874251.1"
                     /translation="MKSKVLHLAFLSLSLISSVGWSSTLSPNTISYGELIRIISGLLF
                     VLFIIIFLSWIVKRMHRVNFTSSKGFQTVASMILGPKEKVVLLKIGDRYLLIGVGSYS
                     INLICDFGNQLPEGFDADQKSSFADVLKSAIGKS"
     gene            4658..5407
                     /gene="fliP"
                     /locus_tag="AC06IO_001049"
     CDS             4658..5407
                     /gene="fliP"
                     /locus_tag="AC06IO_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841869.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HIO9874252.1"
                     /translation="MKIKSWLLILILFLLLPVAGHTDPLSFPVVTMQPGTDGSQSYSI
                     NLQILIFMTLLTVLPGLLMAMTSFTRIIIVLSILRQAIGMAQTPTNQILIGIALFMTY
                     FVMSPVFNKINETAIQPYMNERIDFSLALSNAQKPLRQFMLNQTRKNDLSLFEKFAHN
                     VPKSLDEIPMSVLIPAFITSELKTAFQIGFILFLPFLIIDLVVSSVLMAMGMMMLSPL
                     IISLPFKIMLFVMVDGWTLVLGSLASSFALA"
     gene            5416..5685
                     /gene="fliQ"
                     /locus_tag="AC06IO_001050"
     CDS             5416..5685
                     /gene="fliQ"
                     /locus_tag="AC06IO_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HIO9874253.1"
                     /translation="MTPESVVSLFSDSVYIILLLLAVLILPGLIVGLIISMFQAATQI
                     NETSLSFIPKLFVTFLVLILAGPWLLKTLINYTDTLISNIPYLIG"
     gene            5689..6459
                     /gene="fliR"
                     /locus_tag="AC06IO_001051"
     CDS             5689..6459
                     /gene="fliR"
                     /locus_tag="AC06IO_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HIO9874254.1"
                     /translation="MNLDYQTMIRLMSQFIWPMVRIGGLMLTVPVLSSALLPARIKIL
                     FVFTLSCVCFTYVPHNLSFENFNGLYLVYLVQELLLGLLMGFVLQIVFQVFILGGQII
                     SMQAGLGFAVMVDPTSNASVPLISQLYLMVTTLIFLALNGHLAILETLIESFRIMPIG
                     KSSLDPSVVWSTIMFSGWMFKEAVLISIPAILSLLIVSLSFGVMARVAPQLNLFSLGF
                     PITLLMGFVIIKIGLPTVGTEMVESIKHGIQFITGILR"
     gene            6459..7607
                     /gene="flhB"
                     /locus_tag="AC06IO_001052"
     CDS             6459..7607
                     /gene="flhB"
                     /locus_tag="AC06IO_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HIO9874255.1"
                     /translation="MADEEQSQEKTEQPSSKRLKESRKKGQVARSKDFNATVILLFTG
                     LGFFIFGKQLSLQIASIMQQAFDFDRDILVTGNNSLENLFQLTKSGLWSVVPLLLVIF
                     ILSLLAPLLMGGWVFSGQSIQPKFSRLNVLKGFKRMISLKGFIEMLKAFLKFVLVASA
                     SILVLRSQVPLLLELGKAPLEIAITSGVMILLKSFVLISASLILIAAIDVPFQLYEHS
                     KSIKMTKQELKDEYKETEGKPEVKSAIRRAQQEAARRRMMSEVPKADVILTNPTHYAV
                     ALSYQKKGKKAPVVIAKGKNLVAFQISKVAKEHKIPIISVPALARAIYFSTKLNAEIP
                     RGLYVAVAQVLAYIFQLRDRQRYDYKPEILQNVPIPPELAREAEEEIE"
     gene            7604..9682
                     /gene="flhA"
                     /locus_tag="AC06IO_001053"
     CDS             7604..9682
                     /gene="flhA"
                     /locus_tag="AC06IO_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HIO9874256.1"
                     /translation="MNNVMNYIEYWMRQGFGTPLILIVMISMMMLPLPPFLLDIFFTF
                     NIALSLVVLLAVIYSERPLDFAVFPTVILVATLLRLALNVASTRVVLLNGHNGTDAAG
                     QVIKSFGEVVIGGNYTVGIVVFIILVVINFVVVTKGAGRVSEVSARFTLDALPGKQMA
                     IDADLNAGLINQEQAIKRRTEVAQEADFYGSMDGASKFVRGDAIAGILVLIINIVGGL
                     IIGIFQYNMNFNDAVHNYILLTVGDGLVAQVPSLLLSTAAAIIVTRVSSAQNMGQAVI
                     SQVFAKPRSLVIAAAIIGLIGIIPGMPHIAFILLSAGLAGIAYLFINQEKVKREQQDK
                     EQNEFSQTSDSKLSELSWDDVNPVDVIGLEVGYRLIPMVDNAQGGILLSRIKGVRKKI
                     SQELGFLIPSVHVRDNLDLKPNHYRLSLAGVIMGESMIHPDKWLAINPGQVFGNLEGI
                     PTRDPAFGMDAVWINENQKEKAHTLGYTVVDASTVVATHLSMILEQNSQQLLGYEETQ
                     QLLDKLAISSPKLVKELVPDGLTLGMVSKVLQGLLSEHIPLTDIRTIVETLAEYASKS
                     KDTEYLISQVRIALSRLITQKISGLNEELPIITLKPELEQLLHNTLQNSGHGGVSFEP
                     GMADKIQQALIQFAAQQQAKQESSVLVVQPSIRTVLARFVRTISNDFHVLSYQEIPDN
                     KQIRIIGTVG"
     gene            9723..10862
                     /gene="flhF"
                     /locus_tag="AC06IO_001054"
     CDS             9723..10862
                     /gene="flhF"
                     /locus_tag="AC06IO_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HIO9874257.1"
                     /translation="MKLKRFVAADTRSAMQQIKAAFGPDAVILSSHNIKDGVEIVAAI
                     DFDETVISTQAAVAIAEPPSRTEKYSAQTPLDEMRQEILTLRGMLEAQLRGNVGIHEP
                     LHTVMLQKLLYLGVSPNTATIWARSVKPDLNQQRAWQFILSQVTEQLPIRDSSRIEEG
                     GIYAFVGPTGVGKTTTLAKIAARFALRFGADKLGLVTMDTYRIAAQEQLILYGKILGV
                     KVCIAQDEVSLARVLRQLNDKKLILIDTAGMNPSDERVTKQMTVLSNYLQSIEKVLVL
                     PATSHYQVLIDAIKRYQANHVDQCIITKLDESLAVGGAISAVIESNLALGYLTDGQRV
                     PEDIKIATRHQLVELFAQQELQLFQEERDISKIHPNVRRENYVEA"
     gene            10849..11718
                     /locus_tag="AC06IO_001055"
     CDS             10849..11718
                     /locus_tag="AC06IO_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HIO9874258.1"
                     /translation="MSKLSKGIKDQASGLRNLSRNKPVKVIAVSAGKGGVGKSNISVN
                     LAIALSQLNYSVMLLDADLGLANVDIMLGLHTKYNLSHVLQGICHLSDIILHGPAGVR
                     VIPAASGTEFMTQLSPAEHAGIIDSFNELTDDLDYMIIDTAAGISETVLSFTRSSQEL
                     IVVVCDEPTSLTDAYALIKVMSKRYEWTHFHILANMVRNVKDGRDLFNKLFRVSGQFL
                     DVQLDYLGAIPFDENIHKSVKKQNPILIAYPDSPAAHALRELAESVSNWSFKPSLGGN
                     TSFFLERLVAGQF"
     gene            11815..12531
                     /locus_tag="AC06IO_001056"
     CDS             11815..12531
                     /locus_tag="AC06IO_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HIO9874259.1"
                     /translation="MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLD
                     DLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMI
                     SDAVRILEHKLGREAKDNEIAEELGVSLDDYHDMLQDSISSHLYGFDDLGVTDDVLFD
                     HEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSE
                     SRISQILSQATHRIRSRLPE"
     gene            12541..13314
                     /locus_tag="AC06IO_001057"
     CDS             12541..13314
                     /locus_tag="AC06IO_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HIO9874260.1"
                     /translation="MDTLTLIGLLTGFLAIIVGQIFEGGDLNSLLNFPALLIVMGGTL
                     GAVMVQTPLTTFKRAFRILPWVVKPPHHNFEECRTTLIELSRKARQLGLLSLEDRLDV
                     ETNPLIYKGLELLVTGVDKLTLRQVLEAEIDRTEHQDLRAAHVFESMGGYSPTIGILG
                     AVLGLIQVMRNLADPSQLGLGIAVAFVATIYGVGFANLFFLPVANKLKSCISHKVHFD
                     EMVVEGLVAMASGESPNMLQLKLNNYGQHKQDEIKTKEN"
     gene            13289..14092
                     /gene="motD"
                     /locus_tag="AC06IO_001058"
     CDS             13289..14092
                     /gene="motD"
                     /locus_tag="AC06IO_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HIO9874261.1"
                     /translation="MKSKQKKTEEHQDSHRWVVSYADFITLLFAFFVVMYAISSVNVS
                     KYKSLSEGMKSAFNQKDQNKATQSTDNLKDGPEEKKTKGTFKDGLDELNKSLSELEDG
                     DFKLNRQEGWVEIDIKAGSLFDSGSADIKPEALIKLMKLAEKIKNLPFTVVVEGYTDN
                     VPIETPQYPSNWELSAARAATVGRILNGFGIANHRILVTGYGDQYPITDNYTEAGRSQ
                     NRRVNILITKNRKVDRILNPDLSRVHFSVISNTNSVEFNKKDTKIKDKK"
     gene            14089..14490
                     /locus_tag="AC06IO_001059"
     CDS             14089..14490
                     /locus_tag="AC06IO_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HIO9874262.1"
                     /translation="MTHIFLYLHAIIFLLLGNYLLNQRKNINKLQNKIDSLEKLATQV
                     VKDHPMLINADLLFAKQLNEINQQLISIETQLQELENIRHNDGSYQHALRILEMGGTR
                     EEIISSCHLSNAEAELLMNLQAYRTALKHQN"
     gene            14550..15657
                     /gene="prfB"
                     /locus_tag="AC06IO_001060"
     CDS             join(14550..14624,14626..15657)
                     /gene="prfB"
                     /locus_tag="AC06IO_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031433237.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIO9874263.1"
                     /translation="MLEVNQINLSLIDLNERIESLRGYLDFEGKSERLEEVIRELESS
                     NVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDEQTINDIGEE
                     LKSIEQQVASLEFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEHHG
                     FTTELIECSPGEVAGIKSATIHFTGEYAFGWLRTETGVHRLVRKSPFDSGNRRHTSFA
                     AVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQCQN
                     DRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRIK
                     DLRTGVETSNTQAVLDGSLDQFIEASLKAGVGQA"
     gene            15654..17144
                     /gene="lysS"
                     /locus_tag="AC06IO_001061"
     CDS             15654..17144
                     /gene="lysS"
                     /locus_tag="AC06IO_001061"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIO9874264.1"
                     /translation="MSEEHVHLDESEVYHIRKQKLAELRTGGFNFPNTFRREHLADAL
                     LKQYSETEKQTLEQKHVKVSVAGRIVLRRIMGKASFFHIQDVSGRIQVYLRSNDLPDI
                     YEQFKHWDLGDIVGVQGELFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKY
                     RKRYVDLIANEDSRKTFLIRSHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVT
                     HHNTLDMTMYLRIAPELYLKRLVVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQA
                     YADYNDLMNFTEQLFHYLCDKVLATRQIEYQGQVIDFNKPFERLSVKEAILKYHPDIK
                     AQQLETVEGCRTLLNDLGLPYKETDGLGKLQIILFEETVEHQLFQPTFITEYPTEISP
                     LARRSDTNPEVTDRFEFFIAGREIANGFSELNDAEDQAERFRKQVEEKDAGDLEAMHF
                     DSDYIEALEYGLPPTAGEGIGIDRLVMLFTNSQSIRDVILFPHLRQ"
     gene            complement(17231..17716)
                     /locus_tag="AC06IO_001062"
     CDS             complement(17231..17716)
                     /locus_tag="AC06IO_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1776 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874265.1"
                     /translation="MQSKIHKKEDNTNYSFLLKCMAAATAALATAAIVTAVVIKATAT
                     AAVGFGVATTGAALSPLLFVGGALLAAVGICCLPLLLCCGSSRSVYTVSNSPAYRPWS
                     SAWYTGGYNPTFHSHPVYNNGGTYHTHHSGTVHTHSNPVHTHGNNVHVHTTPSNVHGH
                     R"
     gene            complement(18031..19134)
                     /locus_tag="AC06IO_001063"
     CDS             complement(18031..19134)
                     /locus_tag="AC06IO_001063"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIO9874266.1"
                     /translation="MNYNDTQEIIVTPLMGATGLETLEMGAARIHVDDKQIINCRADL
                     NQLVPFKYKWAWDKYLTACANHWMPNEISMSADVALWKDPYGLTEDERLIIKRNLGFF
                     STADSLVANNLVLAVYRHITNPECRQYLLRQAFEEALHTHAYQYIIESLGLDEAEVFN
                     MYREIPSVSRKAVWALPFTQSLGDETFHTGTPENDQRLLRDLIAFYVVFEGIFFYVGF
                     TQILSMGRRNKMVGTSEQFQYILRDESMHMNFGIDVINQIKIENPHLWTPEFKNEMIQ
                     LIREGVELEYQYARDTMPHGILGMNAEMFEEYLHFIANRRLNQIGLPDQYPGAENPFP
                     WMSEMLDLKKEKNFFETRVIEYQAGGTLSWDDE"
     gene            complement(19148..21976)
                     /locus_tag="AC06IO_001064"
     CDS             complement(19148..21976)
                     /locus_tag="AC06IO_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIO9874267.1"
                     /translation="MSEILEPVKDIPLTNQLELTANVPGMLKTIKRNGKVVIYDDTKI
                     KIAITKAFIADEGGTAPTSDRIHQQIEELTRQITQVFKRRLPSGGAIHIEEIQDQVEL
                     ALMRSGHYKVARSYVLYREERRKARESESKQKQTKDSKHLLITMPNGEVQPLDMDRVN
                     TIVKESCRDLSNVKAEPVIKDALRNLYNQAKYEDVHKALIMSARALVEKEPNYTYVSA
                     RLLLDSLRSEALTKLNIKSEATFDEMAQIYPVYFKSYIEQGIAQGMLDPKMADFDLDK
                     LSQALLPERDMKFTYLSLQTLYDRYFIHERGVRYELPQAFFMRVAMGLALREQHKEEK
                     AIEFYQLLSSFDYMSSTPTLFNSGTVRPQLSSCYLTTVPDHLDGIYSAIKDNALLSKY
                     AGGLGNDWTPVRAMGAHIKGTNGKSQGVVPFLNVADATAVAVNQGGKRKGAVCAYLEC
                     WHRDVEEFLELRKNTGDDRRRTHDMNTALWVPDLFMKRVREDGNWTLFSPDEVPDLHD
                     QYGKAFEALYVEYEEKARQGIIRNTKTISAVKLWRRMLSMLFETGHPWLTFKDPCNLR
                     SPQQHAGVIHSSNLCTEITLNTSEEEIAVCNLGSINLPAHIKNGKLDEQKLKRTISTA
                     VRMLDNVIDINYYSVPQARNSNLQHRPIGLGIMGFQDALYELKMDYASKEAIEFADSS
                     MELISYYAIEASCDLAKERGSYSTYEGSLWSKGILPIDSINLLQQSRNKYLEQDRSQK
                     LDWEPLRVKVRTQGMRNSNVMAIAPTATISNICGVSQSIEPTYQNLYVKSNLSGEFTV
                     VNPYLVADLKALNLWDEVMVNDLKYFNGSVQPISRIPDELKRRYATSFEIDPMWLVEA
                     ASRRQKWIDQAQSLNIYMAQPSGKKLDQLYMHAWIRGLKTTYYLRSLGASNAEKSTVT
                     DGALNAVKLIDTEPKACSITDPDCEACQ"
     gene            complement(22248..23198)
                     /locus_tag="AC06IO_001065"
     CDS             complement(22248..23198)
                     /locus_tag="AC06IO_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HIO9874268.1"
                     /translation="MKLKEIRTIIYNYGEEEILKHILYESTEPILIKIPDFTSQFSLD
                     YFEQLTQAKTTYCTFENKRCVDFQAGNFAEVIQQIKSNKPIRIFGQILSRERSKEIEK
                     HVPLWQKIPFRPRYFRETKKVAYFFGGKGAVSEMHFDREHCCNLHLCLSGKKQVLLFT
                     RKQSDNLYKLPFIGDSLIDFTQPLDALIQQYPRLNQAEGYQVLLEKGDMLFMPRNCWH
                     YTEYLDASSAATYVFYPKKFFQYYGYFTGNFFLGYKEESGFKIYRWPFFEKFSRYYAL
                     STGVSKIVFKIIEKILFLFMLPIISLFNIISHKFNPRRVF"
     gene            complement(23447..24223)
                     /locus_tag="AC06IO_001066"
     CDS             complement(23447..24223)
                     /locus_tag="AC06IO_001066"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIO9874269.1"
                     /translation="MSSQTNVYPANKLNLWLLLVLALAIVIPMTSYAGSDWQELTPGI
                     EYQDLEGGLLNPWSHIHVFRIDLNKNQMALVTAKNLAQKNASVDQFAEHSKALLSING
                     GFFDHEFNPLGLRINNKKQENPLKRISWWGIFYVKDNKPRITNIRNFHYDSNIDFAIQ
                     SGPRLLIRGNIPSLKAGVADRTALGITDDGKVIILVTTNAAMSTRQLAQIMRAPPLSC
                     SDAINLDGGSSSQLYAKIDSFQLNVHGFSNVSDAIIVKKI"
     gene            24269..25627
                     /gene="pmbA"
                     /locus_tag="AC06IO_001067"
     CDS             24269..25627
                     /gene="pmbA"
                     /locus_tag="AC06IO_001067"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HIO9874270.1"
                     /translation="MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNY
                     DRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIA
                     KVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNS
                     DGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENL
                     VSADLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNS
                     FLLDSIGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSY
                     SARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSR
                     GASGYWVEDGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVA
                     GK"
     gene            complement(25794..26015)
                     /gene="infA"
                     /locus_tag="AC06IO_001068"
     CDS             complement(25794..26015)
                     /gene="infA"
                     /locus_tag="AC06IO_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIO9874271.1"
                     /translation="MAKEDHIEMAGTVIDTLPNTMFRVELENGHIVTAHISGRMRKNY
                     IRILTGDKVKVELTPYDLSKGRIIFRDKG"
     gene            complement(26146..>26507)
                     /locus_tag="AC06IO_001069"
     CDS             complement(26146..>26507)
                     /locus_tag="AC06IO_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIO9874272.1"
                     /translation="SQGFLKLVTEAKKRINEISPQILKSKIDNNEKLIIIDVRETDEW
                     ETGKIPNAIHLSKGIIERDIEKIITDNQSNIVVYCSGGYRCALVADSLQKMGYTNVLS
                     LDTGLQGWLEAGYSLVK"
BASE COUNT         8002 a         4580 c         5546 g         8379 t
ORIGIN      
        1 cgcataaaag caaatcattg cgataatgat acttatattt ttcccattct tttataaatg
       61 aaaaaaggga gttttgtaat aattgccatg accactttcc agaaagaagg atagtaaaat
      121 aatcttttat tatgatttat tttttcccaa catgcctttg cacagtcatg aggttgagct
      181 gcgtaaaaaa ctccgggtag tccatacgtt tgttttgtat caatgtatcc aaggcgagcg
      241 attaagatat gttgatgtgg agttgctttt tgctgcaggc cttcgagaaa tatttcaatt
      301 gctgctttac tggcaccata aagactattt ttggcacggc cgcggcagga agcaacagag
      361 cttaaataaa cgatgcgatg tttttcttgt tgggattgta aataggtatt aatcaggatc
      421 gctgtagatg agatattggt atcaattaaa tgtttaatcg attgcggatt taaatggtta
      481 ttatcggtaa aatcacaatg cgcaataaat aaatccaatt ctctttctct ggtttctaaa
      541 atggtaagca attgctcgtt aggctcggat aaattgatag ttatcgcgtt acaagggatt
      601 ttatatcgca attgaatatc ttttgagatt aaggctaatt gctcggagtt gcgtgcaact
      661 aaacataaat ggttaccggc ttgcgcagca aaatgtgcga attgttcggc aataactgaa
      721 gtagctccta aaacaaccca tttcctctgc atcattttat gattcctaaa cgtgtcgcaa
      781 gatctgaacg catggggcat cgatattttg taagtatttg agcaaatttc gattgatttt
      841 cgtacattgt aaaatattgt tctggattta gtaataaatc cttagccaaa taaacacacc
      901 catcgttttc tgcaatgaat tgattcatcg cgcttattgc ttttttggcg tgaggattat
      961 taataaaatc aatagccaga gtaaatccag gccgacaaaa cgaaagcaaa cctaacccag
     1021 gctgtgtaaa tagtttaagg acagtgaggg ttggagtagc actatgagtc ctaataatct
     1081 ctaatatgtg attaataatt tgcgctgcct tatcttgatc aaatacggct tgaaactgta
     1141 taagtcctct aggtccgtag agtcggttcc aatgtgatat tttatccaga ggattattgt
     1201 attgggtgag actcatcatt tcattttctt tttgtctgga gtaaaaatat tgattaaata
     1261 ctttcatgtt ccattttttg ataagtccaa atggtaattt aggcaggcta taaattttta
     1321 attttttatc aggatagttt ggcgattcac aataatttgc tgttgataaa atggctctgg
     1381 gttctgagta taacaagtct aaccaggcta cttggtaatc ttgctgaaga ccataagtcg
     1441 ccatttctgc aattaattct tggatagatg caaatggttt attttttatt gccagatggc
     1501 gtggtgcttt tttaaggcga atagcgactt gtgtaataat gcctgttaaa cctaaacctg
     1561 ctatagttgc gaaaaataga tcactatttt tttcgcggct acaatgcatg atttgatcgc
     1621 ctatcaataa atcaaaccaa gaaatatgat gtccaaaact tcccgctgaa tggttatttt
     1681 taccatgaac atcatgggct attccacctg ctacagtggc aaatgctgtg cctgggagaa
     1741 ctgggggaat gaattcatca tgaagtaaaa acagatcctt aaatggtgtt ccaccctgac
     1801 atactacgat gcctgtttcg acatcaaaat cgatgaaatg attaaagcga ctagtatcaa
     1861 ttaccaggcc gtcttgattt agacagctat cactgtaact caatcctgca cccctggtta
     1921 gcatgttttt aggctgattt tgtgcaatat agtgaataag ttctttttct gtgtctggcc
     1981 ttatacaaca ggattcacta taaattgcgc gggtaaaatt agttaataag attcttttgc
     2041 tgcgcatatc agaaattttt aaatagacag ttgagtgagt gtcgcatgat ttttttatta
     2101 tggaaacagg aacaaaggct ataggatgtc tataattaaa tcaaatatca atggaaggta
     2161 ttctaatgaa caaaatattc aatcttgcca gcatagcctg ctttttattt ttttccttga
     2221 tagcatttgc cgcggatatt cctggtctgg atgagggcac tgataaatta aatatggaag
     2281 aatgtgttgg ggaaaattat cagcaatgcc tggatgatgt ttgtttaacc tctgatgaaa
     2341 tagattgtaa ctccaattgt caaagtttag ctgaagataa atgcgagcaa caagcagtag
     2401 actaagtgga ggattttcta aacatttatt tttgaagtga tttcactacc attaaggaac
     2461 gctcacaacc tcaaatactg tgcataagac ttatgaaaaa ccccaatctc tttgttaaaa
     2521 aatattttta attcagtcat ttagtttatc taaatcctct cgccaaaaca ttttctgcct
     2581 cgactttgga gtttttcgta agtcctatca atataaacct tatgattttt acgtcaaaac
     2641 attggcggag tctaattaaa tgagctaatc aagatttgat tattacacta tctatttgat
     2701 ttattgatat atttcttgga tggcacgggc tttgcataaa cataaccatt ctatagttgt
     2761 tactgattga ttgagctaat tttaaactaa tcaacacttc aattttaaaa taactttgaa
     2821 aatctggttg agatgcgaat ggattgctta tgactgagaa agatgtgtta tcacaagaag
     2881 agatcgatgc attactggat tcagtggatg agtcaattga tgatgaaacg aatgatgaag
     2941 aattgagttc aagagatcag ccagttcgta aaaaaccaga agatactacc agccaggaat
     3001 taaactctaa ttttgattct gacaaaaaag tagcggaaga aggagtcaaa acacttaatt
     3061 ttaccggaca agagcgtatt gttaaaggac agcttccggt attagacaaa atttatgaca
     3121 gggcagttcg tctgtttgca gcagatattt atcatttaac ggctagagat tttgaaatta
     3181 aacaagatcc gctacttatt acaaagcata aggagtttat gaggagttta cctaatccta
     3241 gcctgatagg tatctataaa ttcaagcctc ttcgtggtaa aggaattatt ttgtttgaca
     3301 gtacctttgt gtatgacttg gtcgattatt attttggcgg aaatagccag tttggtgctc
     3361 aaaaggataa aacagatttt actgctactg aacttcgtgt tatggaggtc gtaactaaaa
     3421 aactggtaac taatttaatt catgcctgtg aaccaataat tcaactggat gtaacaaaat
     3481 tcaatgacga aacaaatccc caattagtta atattgccga gccagaagaa atgttgcttg
     3541 tggcgcggtt cgtcttgaat tttggtaagg aaaccggggc tttttacttt attttgcctt
     3601 actccatgct tgaacctatc aaacagcaat tggagctagg ggcatcaaga cctgatgatg
     3661 aaattgatcc taactggatt aattctctga aagaagaact catggacgtt gaattgacgg
     3721 taagtgcttc tatggcggaa acagtcagta ctctgggtca ggtaatggct tggaaagtgg
     3781 gagattttgt ccctttggaa ataaacgagg aggtaactct ggatatagaa gggacaccaa
     3841 gctttactgc aaccttaggc agtgccaatg aaaagcgagc tttgaaagtt attaaaataa
     3901 tacgttatta ggggataagg atgactcaag tgaacgaaac cagcacagag caattaaatc
     3961 aagggaatcc actgaatcca gatcctggca atgagaaatt ggaattaatt ttagatattc
     4021 ctgtctcagt gactgtcgaa atcggccgta ccaaaatgac tattcgtaat ttactgcaat
     4081 taaatcaggg aggaatcgta gcgcttgata ggctggctgg tgatcccctt gatgtgctag
     4141 tcaatggtac tttggtcgca catggtgaag tcgttgtagt taacgataaa tttggtgtca
     4201 gattaactga tatcgttagc aaggctgaac gaataaaacg tctcaaatga aaagcaaagt
     4261 actccatctt gcatttttga gtctttcgct aatcagttcg gtcggttggt catctacttt
     4321 aagcccaaac acgatcagct atggcgaact gataagaatc atttcgggac tcttatttgt
     4381 attattcatt attatttttt tatcttggat agtgaagcgt atgcatagag taaactttac
     4441 ttcatctaaa ggattccaaa ctgttgcaag tatgatttta ggtccaaagg aaaaggttgt
     4501 attgctgaaa attggggaca gatatttatt aataggtgta gggtcatatt ccattaatct
     4561 aatatgtgat tttggcaatc aacttcctga agggtttgat gctgatcaaa aatcgtcttt
     4621 tgccgatgtg ctgaaatcag caataggaaa atcataaatg aaaattaaaa gttggttact
     4681 gattttgata ttatttcttt tattacctgt tgcaggtcac acagaccctt tatcctttcc
     4741 cgttgttaca atgcaacctg gaactgatgg ttcgcaatct tatagtatca atctgcaaat
     4801 tttaattttt atgactttat tgaccgtatt gccaggattg ctgatggcaa tgacgtcatt
     4861 tacaaggata attattgttc tgtcaatttt acggcaggca atagggatgg ctcagacgcc
     4921 taccaatcaa atattaatcg gcattgctct atttatgact tattttgtta tgtcacctgt
     4981 attcaacaaa attaatgaaa cagcaataca gccctatatg aatgaaagga ttgatttttc
     5041 tctggcatta tccaatgcac aaaagccgct tcgtcagttt atgctaaatc aaacgagaaa
     5101 aaatgattta tctttatttg aaaaatttgc tcacaatgta ccaaaaagtt tagatgagat
     5161 tcctatgagt gttctcattc cggctttcat tactagcgag ttaaaaaccg catttcagat
     5221 aggctttatt ctatttttac cttttttaat tattgatctt gttgtgtcca gtgttttgat
     5281 ggctatgggg atgatgatgc tttcaccctt gattatttca ttacctttta aaatcatgtt
     5341 gtttgtgatg gttgatggat ggactttggt tttgggttca cttgcttcaa gttttgctct
     5401 tgcctaggag gctatatgac ccctgaatca gtcgtttcgt tgtttagtga tagtgtttat
     5461 atcattttgc tattactggc agtgttgata ttaccgggat taattgttgg actaattata
     5521 tcgatgtttc aggctgcgac tcaaattaat gagacaagtt taagctttat acccaagtta
     5581 tttgtcacct ttttggttct aattttagca ggaccttggt tattaaagac attgataaat
     5641 tatacagata ctttgataag caatattcct tatttgattg gataaaaaat gaatctcgat
     5701 tatcaaacta tgattcgctt aatgagccaa tttatatggc ccatggtgcg tattggtggt
     5761 ttgatgctta cggtgccagt tttatcttca gcactattac cggcacgcat taaaattctc
     5821 tttgttttta ctttaagctg tgtgtgtttt acctatgtgc ctcataactt gtctttcgaa
     5881 aattttaatg gcctttatct tgtctatctc gtacaggaat tattattggg tttgttaatg
     5941 ggatttgtgt tgcaaatagt atttcaagta ttcattcttg gggggcaaat tatatccatg
     6001 caggcgggtt tgggatttgc ggtaatggtt gatccaacca gcaatgctag tgtgccttta
     6061 atcagtcaat tgtatttaat ggtgaccaca ttaatatttt tggcattgaa tggccatttg
     6121 gctattcttg aaactctaat cgagagtttc agaataatgc caatcggtaa atcttctctg
     6181 gatccatcag ttgtctggag tacgattatg ttttcagggt ggatgttcaa agaagcggta
     6241 ttgatttcaa tacctgcgat tttatctttg cttattgtaa gcttgtcttt tggagttatg
     6301 gctcgggttg ctccgcaatt aaaccttttc tctttaggtt ttccgattac gctattgatg
     6361 gggtttgtca tcattaaaat agggttgccc actgtcggga cagaaatggt agagagcatt
     6421 aagcatggaa tccaatttat aacaggaata ttgcgctgat ggccgatgaa gaacaatcac
     6481 aagaaaaaac ggaacagcct tcttccaagc gattaaaaga atcacgaaaa aaggggcagg
     6541 ttgcacgttc caaagatttt aatgcaacgg tcattttatt atttaccgga cttggtttct
     6601 ttatttttgg gaaacaatta tcactgcaaa ttgccagtat aatgcaacag gcatttgatt
     6661 tcgatcgtga cattcttgtt accgggaata attctttaga aaatttgttt caattaacaa
     6721 agtccgggct ttggtctgtt gttccactat tgctcgttat ttttatttta tctttgctag
     6781 ccccattgct tatgggggga tgggttttta gtggacagtc tatacagcca aaattttctc
     6841 gattaaatgt acttaaaggg tttaagcgca tgatttctct caaaggattt attgagatgc
     6901 ttaaggcttt tttaaaattt gtcctggtgg cgagcgcatc tattctagtg cttagatcgc
     6961 aagtaccctt gttgcttgaa ttggggaaag caccactaga aatagcaatc actagtgggg
     7021 taatgatcct tttaaaatct tttgtgctga tttctgcaag tctgatatta attgcggcaa
     7081 tagatgttcc attccaatta tatgaacata gtaaatcgat taagatgact aaacaggaat
     7141 taaaagatga atacaaagag acggaaggca aacctgaggt aaaaagcgct atacgtcgcg
     7201 cacagcagga ggctgcaaga cgacggatga tgagtgaagt acctaaagcc gacgtcatat
     7261 tgactaatcc tacgcactat gcagttgctc ttagttacca aaagaaagga aaaaaggctc
     7321 cagtggtgat tgccaagggt aaaaacctgg ttgcttttca gatatccaag gttgctaaag
     7381 agcataagat ccctattatt tcagttcctg cgttagctcg cgctatttat ttttcaacaa
     7441 aactgaatgc agaaattccc agagggttat atgttgctgt ggctcaggtg ctggcctata
     7501 tttttcagct acgtgacagg caacgttatg attataagcc tgaaatttta caaaatgtcc
     7561 ctattccacc tgaactggct cgcgaagcag aggaggagat tgaatgaaca atgttatgaa
     7621 ttatatagaa tattggatgc gccaagggtt tggtactcca ttaatactga ttgtcatgat
     7681 cagcatgatg atgttacctc tgcctccttt cctactggat atatttttta cttttaatat
     7741 cgctctttct ttagttgttt tgttggcagt tatttatagt gaaagacccc tggattttgc
     7801 ggtgtttccc acagtgatac ttgtggcaac tttgttaaga ttagctctca atgtagcttc
     7861 tacacgagta gtcttactca atggccataa tggtacagat gctgccggtc aggtcataaa
     7921 atcttttggt gaagtggtga ttggaggtaa ttacacagta ggtatagtgg tatttattat
     7981 ccttgtcgtt attaactttg ttgtagtgac aaaaggggcg gggagagtat ctgaagtaag
     8041 tgcgcggttt actttggatg ctttacccgg aaagcaaatg gctatcgatg cagatctcaa
     8101 tgcaggtcta atcaatcagg aacaggcaat caagcgtcga acagaagtag ctcaggaagc
     8161 ggatttttac ggttcaatgg atggtgccag taaattcgtc cgaggggatg ccatagcagg
     8221 aattttggtt ttaatcatta acatcgttgg tggattaata ataggcatat ttcaatacaa
     8281 tatgaatttt aatgacgcag ttcataatta tattttattg acagtcgggg atggtttggt
     8341 cgctcaagtt ccctccctct tattgtctac tgcagcggca atcattgtga cccgagtttc
     8401 tagcgcccaa aatatggggc aggcagttat ttctcaagta tttgcaaaac caagatcact
     8461 tgtcattgca gcagctatta ttggtctaat aggtataatt cctggtatgc ctcatattgc
     8521 atttattcta ttatcagcag ggttagccgg tattgcttat ttatttatca atcaggaaaa
     8581 agttaaacgt gagcagcagg ataaagagca aaatgaattt tcacaaacca gtgactctaa
     8641 actgagtgag ttatcatggg atgatgttaa tcctgtcgat gtcattggtt tggaagtagg
     8701 ttatcgattg atccctatgg tcgataatgc tcagggtgga atattacttt cgcgaattaa
     8761 aggtgtccga aagaaaatat cgcaagagtt gggtttctta attccatccg ttcatgttcg
     8821 tgacaaccta gatttaaagc ctaatcatta cagattaagt ttggctgggg tgattatggg
     8881 ggaatcaatg attcatcctg ataaatggtt agcaataaac cccggacagg tatttggtaa
     8941 tttagaggga atcccaacaa gagatccggc ttttggaatg gatgctgtgt ggataaatga
     9001 aaatcaaaag gagaaagcac acactttagg ttatacagta gttgatgcat caacagtagt
     9061 tgctactcat ttaagcatga tcctggaaca aaacagccag caattacttg gctatgagga
     9121 aacacagcag ctattggata agttggccat aagttcccct aaactggtta aagagcttgt
     9181 acctgatgga cttactttag gtatggttag caaagtgttg cagggattat tatcagaaca
     9241 tattccatta acagacatac gcactatagt cgaaactttg gcggaatatg cgtcaaaatc
     9301 aaaagatacg gaatatttga ttagccaagt gagaattgct ttatcacgat tgataactca
     9361 gaaaatcagt ggattaaacg aggaattgcc cattattaca ttaaaaccag agttggaaca
     9421 attgttgcac aatactctac aaaacagtgg acatggcgga gttagctttg agccaggaat
     9481 ggcagataaa atccaacaag cactaatcca gttcgctgca caacaacagg caaagcagga
     9541 gagctcagtt ttggtagttc aaccaagtat acgaacggtt ttggcacgat ttgtgcgtac
     9601 aatatccaat gactttcatg ttttgtctta tcaggaaata cctgataaca aacaaataag
     9661 aataattgga actgtaggtt agtttttggg gttgcatagg tgtgttgaag gcaggaggca
     9721 ttatgaaatt aaaacgcttt gtcgctgcag atactcgcag tgccatgcag caaataaagg
     9781 ctgcatttgg ccctgatgcc gttattttgt caagccataa cataaaagat ggtgttgaaa
     9841 tagttgctgc tattgatttt gacgaaacag tgatatcaac tcaagctgct gtagctatag
     9901 cagaaccccc aagccgaaca gaaaaatatt cagcgcaaac gccattagac gaaatgcggc
     9961 aagaaatttt gacactgaga ggaatgctcg aagcacagct gcgtggaaat gttggtattc
    10021 atgagccttt acatacagtt atgctgcaaa aactcttgta tttaggtgtt agtcccaata
    10081 cggcgacaat ttgggctcgc tcagtaaaac ctgatttgaa tcaacaaaga gcctggcaat
    10141 ttattttgtc acaagtcaca gagcaattgc caattcgtga ttctagtaga atagaagaag
    10201 gtggtatcta cgcgtttgtt ggtccaactg gagttggtaa aacaacaact ctagcaaaaa
    10261 ttgcagcacg ctttgcttta cgatttggtg ctgataagtt gggtttggtc actatggaca
    10321 cctaccgaat agcagctcag gaacaattaa ttctctatgg gaagattttg ggagttaaag
    10381 tctgtattgc gcaagatgaa gtctctttgg caagagtatt gcgtcaatta aatgacaaaa
    10441 aattaatttt gattgataca gccggtatga atccttctga tgaacgtgta accaaacaaa
    10501 tgactgtttt aagtaattat ctgcaatcta tagaaaaagt attggtatta ccagctacca
    10561 gtcattacca ggtattaatc gatgcgatta agcgttacca ggcaaatcat gtggatcagt
    10621 gcattattac gaaattggat gaatctcttg cagtaggtgg agccataagt gctgttattg
    10681 aatcaaattt agccttgggt tatttaaccg atggacaacg agtcccggag gatatcaaaa
    10741 ttgctacgcg tcaccagctt gttgagttat ttgcgcaaca agaacttcag ttatttcagg
    10801 aggaaaggga catcagcaaa atacatccga atgttaggag agagaattat gtcgaagctt
    10861 agcaaaggta taaaagatca ggcttctggt cttagaaatt tgtcccgaaa caaacctgtc
    10921 aaagtgattg ctgtttcagc gggcaaaggc ggtgtaggca aaagtaatat ctcggtcaat
    10981 ttagccatag cgttatctca actaaattat agtgttatgc ttctggatgc cgatttaggt
    11041 ttggcaaatg tggatattat gctggggttg cataccaaat acaatttatc tcatgttctt
    11101 caaggcatat gtcatttaag tgatattatt ttacatggtc ccgctggcgt cagggttatt
    11161 cctgctgctt ccggaacaga gtttatgacg cagttgagtc ctgcggaaca tgcgggaatc
    11221 attgattcat ttaacgaact aactgatgat ttggactata tgattattga taccgcagca
    11281 gggatttccg agactgtatt gagttttact cgttcatcgc aagaattgat tgttgttgtc
    11341 tgtgatgaac ctacttcttt gacagatgct tatgcactaa ttaaagtgat gagcaaacgt
    11401 tatgagtgga cgcatttcca cattctggct aatatggtta gaaatgttaa ggatggtcgt
    11461 gacctgttta ataaactgtt tcgggtctca ggacagtttc tggatgtgca gttagattac
    11521 ctcggcgcga ttccttttga cgaaaacatc cataaatcag taaaaaaaca aaatcctatt
    11581 ttaatcgctt atcccgactc acctgctgct catgccttga gagaattagc tgaatcggtg
    11641 agtaattggt catttaaacc gtctttaggt ggaaacacca gtttcttttt ggagcgtttg
    11701 gttgcgggac aattttaaat tattcgctat acttaaaatt agctgtactc tgtttgtttt
    11761 agatttacca aattactaat aactacaatc tgctgttatt gtaaggagag tatcgtggat
    11821 gctttggctg catacagcaa agtaaatcaa caaacccagg aagctctggt aaaaacacat
    11881 gccacattgg taaaacgcat tgcacatcat ctgctcgggc gattaccgca gagtgtacaa
    11941 ctggatgact tgatacaatc agggatgctc ggtctgcttg aagcagcaag acattatgat
    12001 tcatccaagg gggcatcctt tgaaacttat gcaggaatac gtatcagggg acatatgctt
    12061 gatgaagtaa gacgtaatga ttgggtgccg cgttctgttt atcgcaattc cagaatgatt
    12121 tctgatgctg tgcgcatatt ggagcataaa cttgggcgtg aggctaagga taatgaaatt
    12181 gctgaagagc taggtgtgtc attagatgat tatcatgata tgttgcaaga ctccattagc
    12241 agtcatttat atggttttga tgacttggga gtaaccgatg atgtcttatt tgatcatgaa
    12301 gggaatggct caactgaacc gcacaggaat gttatgcgaa gtgatatgat gaatcgatta
    12361 acccaagtga ttgatagtct ccctcgtaaa gagaggctgg tgctttcttt gtattatgag
    12421 caggatttga atttgaagga aatcggggaa atactggagg ttagtgagtc acgaatttca
    12481 cagattttaa gtcaagcaac gcaccgtatt agatcaagat taccggaata aaaaataagt
    12541 atggatactt tgactttaat aggattgttg acaggttttc tcgccattat cgtaggccaa
    12601 atttttgagg ggggagattt aaattcctta cttaattttc cggccttact gattgtcatg
    12661 ggtggaacat taggggctgt tatggtgcaa actcctttaa ccacttttaa aagggcgttt
    12721 agaatattgc cttgggtagt gaaacctcct catcataatt ttgaagaatg cagaacgact
    12781 ctcatagagt tatctagaaa agctcgccaa cttggccttc tctccctaga agatcgtttg
    12841 gatgtcgaaa ccaatccttt aatttataag gggttggaat tactggttac aggagtggac
    12901 aagcttacct tacgacaagt tttggaagca gaaatagatc gaacggagca tcaggatttg
    12961 cgtgctgcgc atgtctttga aagcatgggc ggctatagcc ccaccatagg aattttaggt
    13021 gcggtgttgg gtttaattca agtcatgagg aatctcgctg atcccagtca attaggttta
    13081 ggcattgcgg ttgcttttgt tgcaactatt tatggtgttg ggttcgcgaa tctgtttttt
    13141 ttacctgttg ctaataaatt aaaaagttgt atttctcata aggtgcattt tgatgaaatg
    13201 gtcgttgaag gcctggtcgc catggcgagt ggtgagagtc ccaatatgct gcagttaaag
    13261 ttaaacaact atgggcaaca caagcaagat gaaatcaaaa caaaagaaaa ctgaagaaca
    13321 tcaggatagt catcgatggg tcgtatctta tgctgatttc attaccttat tatttgcttt
    13381 ttttgttgtg atgtatgcta tttcttcagt aaatgtatca aaatataaat ctctttctga
    13441 aggaatgaag tcggcattta atcaaaagga tcaaaataaa gcgactcaat cgacagataa
    13501 ccttaaagat ggtcctgaag aaaagaagac caagggaact tttaaagatg gtttggatga
    13561 actgaataaa tcactatctg aacttgaaga tggtgatttc aaattaaaca gacaagaggg
    13621 atgggttgag atagatataa aagcaggctc attatttgat tctggtagtg ctgatattaa
    13681 accagaagca ttgattaaac taatgaagct ggcggagaaa attaagaatc taccttttac
    13741 agtggttgtt gagggataca ctgataatgt gcctattgaa acgccccaat acccttcaaa
    13801 ttgggaatta tcagctgcaa gagcagctac tgttggtcgc attttaaatg gttttggaat
    13861 tgccaatcat cgtattttgg ttacaggata tggtgatcaa tacccaataa cggacaatta
    13921 cactgaagct ggaagaagcc agaatcgaag agtgaatatt cttatcacta aaaatagaaa
    13981 agtggacaga attctcaatc cagatttaag tagagtccat tttagtgtta taagtaatac
    14041 aaattcagta gagtttaata aaaaggatac taaaattaag gataaaaaat gacacatatc
    14101 tttctgtatt tgcatgcgat aattttttta ctcttgggta attatttatt aaatcaacga
    14161 aaaaacatca ataaattaca gaacaaaata gattctttgg aaaaattggc tactcaagta
    14221 gtaaaagatc atcctatgct gatcaatgcg gatttattat ttgcgaaaca acttaatgaa
    14281 attaatcagc aattaattag tattgagact caattgcaag aattggaaaa cataagacat
    14341 aacgatggca gttatcaaca tgccttgaga atcctggaaa tgggtggcac tcgtgaggaa
    14401 attatcagta gttgtcattt atcaaatgct gaagcagagt tactaatgaa tcttcaggcg
    14461 tatagaaccg cattaaaaca tcagaattag tatagataga atcttacttt cgctgtaaaa
    14521 tctcctattt atttttaaaa gagacactga tgctagaagt gaatcaaatt aatttaagtt
    14581 taatcgatct taatgagcgc atagaatcgc ttagggggta tctttgactt cgaaggtaaa
    14641 agtgagcgct tggaagaagt aattcgcgaa ttggaatcat ccaatgtgtg ggacaatccg
    14701 gaacaggctc aggcattggg acgtgaaaga actcagctgg aaactatagt ccactcatta
    14761 agccaattaa gccaatcaat tagtgatcag agggaactgt ttgaattggc tcgagaagaa
    14821 gatgatgagc aaaccatcaa cgacattggt gaggaattaa aatcaattga gcaacaagtc
    14881 gccagtttgg aatttagaag aatgttttct ggcaaaatgg accactccaa tgcttatctt
    14941 gatattcaag caggttctgg cggcactgaa gcgcaagatt gggctgaaat gttattacga
    15001 atgtatttac gttggggaga acatcacgga tttaccaccg agttgataga atgctctcct
    15061 ggtgaagtgg caggaattaa aagtgcgact attcatttta caggtgaata tgcgtttggt
    15121 tggttaagga ctgaaacagg tgtccatcgt ttagtgagaa agtcgccatt tgactctggc
    15181 aacaggagac acacttcgtt tgcagctgtt ttcgtctctc ctgaaattga cgatgatatc
    15241 gaaattgaaa ttaatccagc tgatttgcgg attgatactt acagagcttc cggggctggt
    15301 gggcagcatg ttaaccgtac tgattctgcc gtaagaatta cacatatccc tagtgggata
    15361 gtcgttcagt gccagaatga tcgaagccag cataaaaata aagaccaggc aatgaagcaa
    15421 ttgagggcaa agctttatga gttggaaatg caaaagaaaa atgcggaaca gcaagcattg
    15481 gaagctagca aatctgacat tggttgggga tcacaaattc gttcttacgt attagaccaa
    15541 tctcgtataa aagatttgcg caccggtgtt gaaaccagta atactcaggc agtattggat
    15601 ggttcattag atcaatttat tgaagccagt ttaaaggcag gagtagggca agcatgagtg
    15661 aagaacatgt acatttagat gaaagtgaag tttatcatat tcgcaagcaa aaattagcgg
    15721 aattgcgcac aggtgggttt aattttccaa acacttttcg ccgcgaacat ttagccgatg
    15781 ctttgctgaa gcaatactct gaaactgaga aacaaacatt agaacaaaag catgtgaaag
    15841 tatctgttgc aggccgaatc gttctgagaa gaattatggg taaagccagt ttttttcata
    15901 ttcaggatgt atcaggtcgg atacaagttt atcttcgttc aaatgatctt cctgacattt
    15961 atgaacagtt taaacattgg gatttaggag atattgttgg tgtacagggt gagttgttta
    16021 aaacaaacac tggtgaactg accattaatg cagaacatgt tgagttgtta accaaatctt
    16081 taaggccact tcctgataaa ttccatggtt tggctgatca ggaattgaaa tatcgaaaac
    16141 gttatgttga cttgattgca aatgaagaca gtcgaaaaac ttttctaata cggtcacatt
    16201 taatcaaagc cttcagagaa tttatggatg ataatcattt tctggaagta gaaacaccaa
    16261 tgatgcatcc aattcctgga ggtgctttgg ctcgcccttt cgtaactcat cacaatacat
    16321 tggatatgac catgtatttg aggatagccc ccgaattgta cttaaaacgc ttggttgttg
    16381 gtggttttga gagagtatat gaaattaata gaaatttccg taacgaagga atttctactc
    16441 gtcataatcc agagttcacg atgctggaat tttaccaggc ttatgccgat tacaatgatt
    16501 tgatgaattt tactgaacaa ttgtttcatt atctatgcga taaagtatta gctactcggc
    16561 aaatagaata tcaaggccag gtcattgatt tcaataaacc ttttgaacgg ttgagcgtaa
    16621 aagaagctat attaaaatat catcctgata ttaaggcgca gcagttagaa acggtagagg
    16681 gatgcagaac acttctgaat gatttaggtc tgccttataa agaaactgat ggcctgggta
    16741 aactacaaat tattcttttt gaggagacag tagagcacca attatttcag cccactttta
    16801 ttacggaata ccctacagaa atatctcctt tagcgagacg ttctgatacc aatccggaag
    16861 tcaccgatcg atttgagttt tttattgctg gtcgtgagat tgcgaatgga ttttctgaat
    16921 taaacgatgc ggaggatcaa gctgagcgct ttcgaaagca agttgaagaa aaggatgcgg
    16981 gtgatttaga agcaatgcat tttgatagtg attatattga agcattggaa tatggcttgc
    17041 cgcctacagc tggtgaaggg attggaatcg atcgcttggt gatgttattt actaattcgc
    17101 aatcaatcag agacgttatt ctatttccgc atttgagaca gtaaagtaca gcgattttta
    17161 gatagcaatg gtttgtttag aaccattgct atgtcaagca atacataaaa tacacttggt
    17221 ttagataaaa ttatctatgt ccatgaacat tacttggagt agtatggacg tgaacattat
    17281 taccatgtgt atgtactgga ttagagtggg tatgtacggt tcctgagtga tgagtgtggt
    17341 aagtaccgcc attgttataa acaggatgtg aatgaaaagt tggattatag cctcctgtat
    17401 accatgcact gctccaaggc ctgtaggctg gtgagtttga cacggtatat acactacggc
    17461 tgctgccaca gcagagcaat aatggaaggc agcagatacc cacagcggcc agtaatgctc
    17521 ctcctacaaa taataatggc gatagagcgg ctcctgttgt tgcaacacca aaacctactg
    17581 ccgctgtcgc agtggctttg attaccacag cggttactat tgctgctgta gcaagtgccg
    17641 ctgtagcagc agccatacat tttaatagaa agctgtaatt tgtattgtct tcttttttgt
    17701 gtattttaga ctgcataact tacctcatct tgattttcag ttaatagaat gcatttttct
    17761 gggtgtgtta tatcttttag cgatgttgtt attattttgc tggcatagca ttatttctat
    17821 gcgcttggga ttttacctga tcaaactaaa atgtcaccat aaatttggat ttagaggttg
    17881 atttgtttaa aattaattca aaaataatgg aaatacgtag aatattatta agtttggtta
    17941 agttaaagac aggggtatga catttttcac aaagtaaggg atttctctaa atgaactccc
    18001 ttactttgct agaggtaaca tcattaaact ttattcatca tcccagctta aagtaccacc
    18061 agcctgatat tcaataacac gagtttcaaa aaagtttttc tcttttttga gatccagcat
    18121 ttcactcatc cagggaaatg ggttttcagc acctgggtat tgatccggca gacctatttg
    18181 atttaagcga cggtttgcaa taaagtgcaa atattcttca aacatctcgg cgttcattcc
    18241 gagaatgcca tgaggcatag tgtctctggc gtattggtat tcgagctcaa caccttctct
    18301 gatgagttgg atcatttcat tcttaaattc aggtgtccat aagtgaggat tttcgatttt
    18361 gatttgatta atcacatcaa taccgaaatt catatgcatg gactcatcgc gcagtatata
    18421 ttggaactgt tctgatgttc ctaccatttt gttacgacgt cccatagaga gaatttgagt
    18481 aaatccgaca taaaagaaaa taccctcaaa cacgacataa aatgcaatta aatcgcgtag
    18541 caggcgttga tcattttctg gagtgcctgt atggaaggtt tcatcaccta aactttgagt
    18601 aaatggtaat gcccaaactg ctttcctgga tacagaaggt atttctctat acatattaaa
    18661 tacttcagct tcatccagcc ccaaactctc tataatgtat tgataagcat gagtgtgtaa
    18721 cgcttcttca aaagcttgcc ttagaagata ttgtcgacac tcaggattag taatatgtct
    18781 gtaaacagca agaaccagat tattcgcaac gagtgaatcg gcggttgaga aaaatcccaa
    18841 attacgctta atgattaaac gctcatcttc tgtaagacca taaggatctt tccataaagc
    18901 gacatcggca ctcatactga tttcattggg catccaatga ttggcacagg ctgtcaagta
    18961 tttatcccaa gcccatttgt atttgaaagg caccaattga tttaagtcag cacggcaatt
    19021 aatgatttgc ttatcatcaa catgaatgcg tgcagccccc atttctaaag tttcgagccc
    19081 tgtcgcgccc ataagaggag ttacgataat ttcttgagta tcattgtagt tcataaattt
    19141 ctcctgatta ttggcaagcc tcgcagtctg gatcagtaat agaacaagcc tttggttcgg
    19201 tatctattaa tttgactgcg ttaagtgccc cgtcggtaac agtggatttt tcagcattac
    19261 ttgcacctaa actacgtaag tagtaagtgg tttttaatcc acgaatccat gcatgcatat
    19321 aaagctgatc cagtttttta ccagaaggtt gcgccatata gatgttcaag gactgggctt
    19381 gatctatcca tttttgacgg cgtgacgcgg cttcaaccaa ccacatgggg tcaatttcaa
    19441 aagaagtggc ataacgtctt ttcagctcat ctggaattcg actgataggt tgtacactgc
    19501 cattaaaata ttttagatca ttgaccatga cctcatccca aagattcaga gcttttaaat
    19561 cggctaccaa ataaggattc accacggtaa attcgccgga caagttggat ttgacgtaaa
    19621 ggttctgata agttggttca atagattgtg atacaccgca gatgttggat attgtggctg
    19681 taggtgcaat agccattaca tttgagttac gcatgccttg tgttcgaact ttaactcgta
    19741 atggttccca atccagtttt tgtgacctgt cttgctccag atatttatta cgggattgtt
    19801 gcaagaggtt aattgaatcg ataggtaata tgcctttgct ccatagagac ccctcatagg
    19861 ttgagtaact gccccgctct ttagccaggt cacaagaggc ttcgatagca tagtaactga
    19921 ttagttccat ggaagagtca gcaaattcaa tggcttcttt ggatgcataa tccattttta
    19981 attcgtatag cgcatcctga aagcccatga tacctagacc aattggccga tgttgcaagt
    20041 tggagttacg agcttgaggt acagaataat aattaatatc aatgacatta tccaacatac
    20101 ggacagctgt tgatattgtg cgcttgagtt tttgctcatc cagtttaccg tttttaatat
    20161 gcgctggaag attgatactg cctaaattgc acacagcaat ttcttcttca gaagtattta
    20221 gagtgatctc tgtacataaa ttagaactgt gtattactcc agcatgttgt tgaggtgatc
    20281 gtaaattgca gggatctttg aatgttagcc atggatgacc agtttcaaaa agcatggaaa
    20341 gcattctacg ccatagtttg acagctgaaa tggtttttgt attcctaata ataccttgtc
    20401 gagctttttc ttcatattca acatagagag cctcaaatgc tttaccatat tgatcatgta
    20461 aatcgggtac ttcatcaggt gaaaataaag tccaattacc atcttcacga acacgcttca
    20521 taaacaaatc aggtacccaa agggcagtat tcatgtcgtg agtacggcgg cgatcatcac
    20581 cggtattttt tcttaactcc agaaattcct ctacatctct gtgccagcat tccaggtacg
    20641 cgcagacagc tcccttgcgt ttccccccct gatttactgc tacagcagta gcatcggcaa
    20701 cattcaggaa aggaactacg ccttgagatt taccattagt ccctttgata tgggcaccca
    20761 tagctcgtac tggagtccag tcattgccta gtcctccagc atatttagag aggagggcat
    20821 tgtcttttat agcgctataa ataccatcca aatgatcggg tactgttgtt aaatagcaac
    20881 tggataactg cggccttact gttccggaat taaataaggt tggagtggaa gacatataat
    20941 caaacgaaga aagcaactga taaaactcta tggctttctc ttccttatgt tgttcacgaa
    21001 gagcaagacc catggctact cgcatgaaaa aagcttgagg aagctcatat cttaccccgc
    21061 gctcatgaat aaaataacga tcatataaag tttgtaaact taaataggtg aacttcatat
    21121 ctcgttcggg aagaagagct tggctaagct tgtccaggtc gaagtcagcc atcttggggt
    21181 caagcatacc ttgggcaata ccttgctcta tgtaagattt gaaataaact ggatagattt
    21241 gagccatttc atcaaatgta gcttctgatt tgatattgag tttagtcagt gcttcagaac
    21301 gtaagctgtc tagcaacaaa cgcgcgctga cataagtata attaggttct ttttcaacca
    21361 acgcacgcgc tgacataatc aaagctttat ggacgtcttc atatttggct tggttataaa
    21421 gattgcgcaa agcatcttta ataacaggtt ctgctttgac gttgctgagg tcacgacatg
    21481 attctttgac gatagtattg accctgtcca tatctaatgg ttgtacttct ccattaggca
    21541 tagttattaa taaatgctta ctgtcttttg tttgtttttg tttcgattct gattcacgtg
    21601 cttttctgcg ttcttctcta tataaaacat aggagcgagc tactttataa tgtccgctgc
    21661 gcataagggc gagttcaact tgatcctgga tctcttcaat atgaatagcc ccaccactag
    21721 gtaaacgacg tttaaatacc tgggtgattt gtctggttag ctcttcaatt tgctggtgaa
    21781 ttcgatcaga agttggggct gtaccacctt cgtcggcaat aaaggctttg gtaatagcaa
    21841 ttttgatttt agtatcatca taaatgacta ccttgccatt tcgttttatt gttttaagca
    21901 tgcctgggac attagcagtc aattccagtt gatttgttag cggtatatcc tttaccggct
    21961 caagaatttc ggacattttt cctccacgaa atatatttta gcaattcatt gtttcagtga
    22021 atccaacatg ttttacgaac ttgtttaaaa ggcgatccta gtttcattga aactatgatt
    22081 tgctcctgtt tttacgacag tcaagagaag cttttgttta ctgttaccat aattatagca
    22141 aaaaacccaa tatatagggt gttttagggg ttttaataac aagataatgt gtttttttgc
    22201 tttggtcaat agaataatct gggattattc tgtggataag ttgtgggtta aaatactctg
    22261 cgggggttaa atttatgaga aataatgtta aataggctaa ttataggcag catgaataga
    22321 aataaaattt tttctattat tttaaacacg attttggaga ctcctgttga taatgcatag
    22381 taacggctga atttctcaaa aaaaggccat ctataaattt taaaaccgga ttcttcttta
    22441 taccctaaaa aaaaattgcc ggtaaaatat ccataatatt ggaaaaattt tttaggataa
    22501 aaaacataag tagcggcaga tgacgcatca agatactctg tgtaatgcca acaatttcta
    22561 ggcatgaata acatatcgcc tttctcaagt agaacctgat atccttcagc ctgattcagt
    22621 ctggggtatt gttgaattaa agcatccagg ggttgagtga agtcaattaa agaatctccg
    22681 ataaagggga gtttgtaaag gttgtcactt tgttttctgg taaacagcaa tacttgtttt
    22741 ttcccactca aacataaatg gagattgcag caatgttctc ggtcaaaatg catttctgag
    22801 acagcgcctt tacctccaaa gaaataagcc acttttttgg tctctctaaa ataccttggt
    22861 ctgaaaggaa ttttttgcca aagtggcaca tgcttttcta tttccttaga gcgttctcta
    22921 gacaaaattt gcccaaaaat acgaatgggt ttgttgcttt ttatttgttg gatgacctca
    22981 gcaaaattgc ccgcttggaa atcaacacat cgcttgtttt caaaggtaca ataagtagtt
    23041 ttggcctgag tgagttgttc aaaataatcg agtgaaaatt ggctggtaaa atcaggtatt
    23101 ttaattaaga taggctctgt ggattcatac aagatatgct taaggatttc ttcctcgcca
    23161 tagttgtaga tgattgttct gatttctttt aatttcatta aattacctgt tgaatggagt
    23221 aatttataaa tatttttatc tcaataatct atattatatc attaaaaaaa tcatggcttg
    23281 attgtgattt atgcatacga atgatatagc agattcggga ttacggatct cgcaaaaacg
    23341 aaggtggaat ttgaaaaggg ggttctgcag gtggttggag cgcgtgaatt taattctata
    23401 aggagtttga acccaaccga ttataagggg cttgccttga atgatactaa attttcttaa
    23461 ctatgatcgc atcactgaca ttagaaaaac catgaacatt gagttggaag ctatcaatct
    23521 tggcatataa ttggctggaa cttccaccat ccaaattgat tgcgtcacta caggataatg
    23581 gaggtgctct cataatttga gctaattgtc tggttgacat ggcagcattt gttgtaacta
    23641 atataatcac tttgccatcg tccgtgatcc ctaacgctgt tctgtctgca accccggctt
    23701 taagagaagg gatgtttcct ctgattaata atctggggcc gctttgaata gcaaaatcaa
    23761 tgttactgtc ataatgaaaa ttgcgtatat tagtaatacg aggcttatta tccttaacat
    23821 aaaaaatgcc ccaccagcta atgcgcttta gtggattttc ctgttttttg ttgttaatcc
    23881 taagtcccag aggattaaat tcatgatcaa aaaacccgcc atttatgcta agtaaagctt
    23941 tgctgtgttc ggcaaactga tcaacggatg catttttttg agcaaggttt ttggctgtca
    24001 ccagtgccat ctgattttta ttaagatcga tacgaaatac atgaatatga gaccaagggt
    24061 ttaacaagcc tccttcgaga tcctgatatt caatacctgg agtcagttct tgccaatcac
    24121 tgccagcgta ggaagtcata gggatgacaa tggcgagtgc taaaacaagc aagagccata
    24181 aattcaattt gtttgcagga taaacattgg tttgcgaaga catgttattg tatccttatg
    24241 tacatcattc aacaccacgg aatctcatat gcaaattaaa ccgcaaaata acaatagtga
    24301 agtatataag tcaacaacag atttaaatcg agtaataaaa gatgttttag aacttgccaa
    24361 aaaagaaggt gccactgatg cagcagtagc agtaaattat gatcgaggtt tttcagtcga
    24421 tgtgagaatg ggggaagtgg agacagtcgc ttttagcgaa gataaaggag ttggtttaac
    24481 cgtatacata ggtcagcgca aagggggagc aagcagtaca gatacctctc ctgaagcatt
    24541 acaatctttg gttaaagcag cctgtgagat agcgaaggtt agtgccgaag atccttgctt
    24601 tggattagct gataaggaat tgatgacaaa gaatcatcca gatcttgatt tgtatcattc
    24661 ctgggacatt accccacaac acgctataga tttggcttta aaatgcgaat cacatgcctt
    24721 gtctttagat aaacggatta ctaattcaga tggtgttaat gtttcttcct atgaatctca
    24781 tcacggttat gccaataccc atggtggaga ggggtttatt catagtactc gacatagcgt
    24841 aagttgctcg gtaatcgcca aagaaggtga tgaaatgcaa agagactacg attatacgac
    24901 tgccagaaat gcagaaaatt tagtctcagc tgatttgata gcagctaaag ctgttgaaag
    24961 ggcattaagt cgtctgggat caaaacaaat taaaacacaa atgactccgg ttattttttc
    25021 ttcaagagtt tccagtggat tattgggtag ctttgtgagc gctattagtg gttcaaattt
    25081 atatagaaaa aattcctttt tgcttgattc cattggacaa caattgtttc cggagtttgt
    25141 tcatatttac gaacagccac atttaaaagg tgctttgggg agcgcccctt ttgatgggga
    25201 aggtgtgcct acgcgaccca atgtattagt tgagaagggg cgcttgcttc aatatgttct
    25261 tggcagttat tcggcgcgaa aaatgggttt aaggacaaca gcaaatagtg atggcgttca
    25321 taatttaacc attgatccta cttgcgggga tcttggtgac cttttgaaaa tgatgcggaa
    25381 agggttgctg gttacagaac taatgggtca gggtgtgaat ggtattaccg gagattattc
    25441 acgtggggca agtggatact gggttgagga tggggtcata caatatccgg ttgatcagat
    25501 tactatcgca ggaaatctta aagaaatgta ccggatgata ttggcagtag gcagtgacat
    25561 taatccgaat atttccatgc gctgtggctc tattttaata gaaaatatga tggttgcagg
    25621 gaaataaaat gccccaaacc atcgaggtta accacttgct ctgtaatctt gatgatagtt
    25681 ctctggaaca tttttagcga tatacctggc tgcttgcagc cagctttttc tatattccgg
    25741 cagattttct ttaatgaaac agagaacagc tatagaaatt ggatgaaacg catttagcct
    25801 ttatcgcgaa aaatgatacg accttttgaa agatcgtaag gagtcagctc taccttaacc
    25861 ttatccccag tcagaattct gatataattt ttcctcatcc gacctgaaat gtgagccgta
    25921 acaatatgtc cattttccaa ctcaacgcga aacatggtgt tgggtagcgt atctataaca
    25981 gtaccagcca tttcaatgtg atcttctttt gccatatgtc tctcaaatga tgtatcaaat
    26041 ttttaaaagt gaaatagtgc actaaattgc ggaaaatggc aatccagtct tgcaattaca
    26101 ttgttcaaag atatagctca aggctcatta ttgggagata ttcaattatt ttaccaagct
    26161 gtatcccgcc tctagccatc cttgcaatcc agtatccaga gacaatacat tggtatatcc
    26221 cattttttgt agactatcgg ctaccaaagc acagcgataa cctccgctgc aataaactac
    26281 aatattgctt tgattgtctg tgataatttt ttcaatgtca cgctcaataa tccctttact
    26341 taaatgaatt gcgttcggga ttttacctgt ttcccattca tctgtttcac gaacatctat
    26401 tatgattaat ttttcattgt tatcgatttt actttttaaa atttgaggac ttatctcatt
    26461 tatacgtttt tttgcttctg ttaccagttt taaaaaacct tgtgaat
//
LOCUS       DBNCWK010000027        26471 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.027, whole genome shotgun sequence.
ACCESSION   DBNCWK010000027 DBNCWK010000000
VERSION     DBNCWK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26471
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.027"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            76..2007
                     /locus_tag="AC06IO_001070"
     CDS             76..2007
                     /locus_tag="AC06IO_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HIO9874273.1"
                     /translation="MNTQDFLAGYTKRFVDNKEEDMSLDEYLELCKTDPTAYANPAER
                     LLMAIGEPELIDTRHDPVLSRIFSNKIIHHYEVFKDFYGMEEPIEQIVGFLKHAAQGL
                     EETKQVLYLLGPVGGGKSSIAEKLKDLMERIPFYAIKGSPVFESPLSLFNPLEDGELL
                     RERFGIPTRYLRYLMSPWAVKRLQEYNGDISKFRVIKVKPSRLKQIAIAKTEPGDENN
                     QDISSLVGKVDIRKLEEFSQDDPDAYSYSGGLCRANRGLLEFVEMFKAPIKVLHPLLT
                     ATQEGNYNATEGLSAIPFEGIILAHSNESEWQSFRNNKNNEAFIDRINIVKVPYCLRV
                     SEEIKIYQKLIHNSSLANAPCAPGTLDMLAQFSVLTRLKEPQNSSIYSKMRVYNGESL
                     KDTDPKAKSYQEYRDFAGVDEGMSGISTRFAFKILSKVFNFDHTEVAANPVHLMYVLE
                     RQIEQEQFPQELHETYLNFIKEYLATKYVDFIGKEIQTAYLESYSEYGQNIFDRYITY
                     ADFWIQDQDYRDPDTGEIFDRALLNLELEKIEKPAGISNPKDFRNEVVNFVLRARANN
                     RGKNPVWNSYEKLKSVIEKKMFTNTEDLLPVISFNAKASEEDKKKHEEFISRMVEKGY
                     TRKQVRLLCEWYLRVRKSQ"
     gene            2161..3426
                     /locus_tag="AC06IO_001071"
     CDS             2161..3426
                     /locus_tag="AC06IO_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HIO9874274.1"
                     /translation="MSQLIDRRQNAGKKSTVNRQRFLRRYKSQIKKAVSEAVGKRSIT
                     EIDQGEQITIPAKDIYEPQFHRGHGGHIERVLPGNDNFIAGDRIKRPGGGGSGGAGGN
                     ASDSGEGEDNFVFELSREEFLELYFEDLELPDLVKKELARISTYKTVRAGVTTSGIPN
                     NINVLRSMKQATGRRVALASPYKRRLKEAEEELERLKQLANPDKLDILKLERDIEFFK
                     KKIQTVPFIDTIDLRYNHRVRVPSPSTQAVMFCVMDVSGSMDEAKKDIAKRFFILLYM
                     FLTKNYEKIELVFIRHHTSAKEVNEEEFFYSRETGGTVVSSALELLNTIIEARYPPQA
                     WNIYVAQASDGDNWNADSPYCQELLQEKIMPLLQYFAYIEIMPRHHQSLWEVYQQVKE
                     RYPNFAMENIDNVADIYPVFRELFKRKTV"
     gene            3423..4946
                     /locus_tag="AC06IO_001072"
     CDS             3423..4946
                     /locus_tag="AC06IO_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HIO9874275.1"
                     /translation="MRKKPLSTGAEWTFELIQAYDREIARIAKSFNLDTYPNQIEIIT
                     AEQMMDAYSSVGMPIGYHHWSFGKHFVSVEKSYKRGQMGLAYELVINSNPCISYLMEE
                     NTMAMQALVIAHACYGHNSFFKNNYLFKMWTSADAIIDYLVFAKKYISDCEERYGIDA
                     VEPVLDACHALMNYGVDRYKHPTPLSIQEEKIRQQNREIYMQSQVNELWRTIPFSKQI
                     DKNGQKKRFPEEPQENILYFIEKNAPLLEPWQREIVRIVRKLAQYFYPQGQTKVMNEG
                     WACFWHYTILHALYDEGLVTDEFMLEILQSHTNVIMQPAYNSPYYNGINPYTLGYHMM
                     QDIKRICENPTDEDKKWFPYLANTDWLTSLDTAMRNYKDESFIAQYLSPKLIRDLKLF
                     HIVDDDRNSELYINAIHDEWGYQQIREALSRQYNLGYLEPDIQVYSVDLQGDRSLTLR
                     YSQQNRVPLGSSTNEVLKHLHYLWQFPVILQAVDGENKITEEFSCPPLNKNKTAETN"
     gene            5330..6190
                     /gene="vipE"
                     /locus_tag="AC06IO_001073"
     CDS             5330..6190
                     /gene="vipE"
                     /locus_tag="AC06IO_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipE"
                     /protein_id="HIO9874276.1"
                     /translation="MPLTQTQRLINTYGASLKNGTISNEELIILLDPNTFTKSEGYVD
                     PNAPVSDSNHSKMDAIKDFVLTIGSTLDSEILHQLTSRMIELSPPGDRNTFMRGSSLE
                     KAFLAFEMAHYPTKAEEHFNSTRVRTEFPGENDIDNLKAVILNPIIAFFQSPPGTDEK
                     LEKLIKLCTDYKEHLKPSLTDDANNLVNKKHAIVTNMLAALTDSTAPNSATKIKNMEQ
                     ILTEENKETLKDRRDSFSGARFLESILHILSLGIYSKLTKGTFKFWKSHGEALSDNIE
                     EVSKQQPGMK"
     gene            complement(6271..7476)
                     /gene="lapA"
                     /locus_tag="AC06IO_001074"
     CDS             complement(6271..7476)
                     /gene="lapA"
                     /locus_tag="AC06IO_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase LapA"
                     /protein_id="HIO9874277.1"
                     /translation="MRFNQWMTCITSGLILASNSSFATTSPVHEQLQVPQCLAAKITV
                     PHKILAENKEFKIIDVLSSDVETLTILADKVSCGHFVNVSHKLTGTLAANQQQSAQKL
                     LQKKLVKPFGVSKLHKDVYEIKHEEEVNAALKEIVSDNIWQTLTHMTSYYNRSATKDT
                     GVETANWLKSKFEQMAVEYGRTDTSTFFVKTGWYKQPSLVTVIGKDIKAPAIVIGAHM
                     DTLDGRMPGAGDDGSGSSSIMEAARVILSSKTTFKRPIYFIWYAAEERGLVGSQHVVQ
                     HFQEQSIPVKAVVQFDMTGYRNDANDPTMWVFTDYTDRDLSNYLAKLIDHYIHVPVDY
                     SRCGYGCSDHASWNEEDIPAAFPCETSFADHNPYIHTSSDKMDLLNLEHMTNFSKLAV
                     AFAIELASE"
     gene            7658..9640
                     /gene="iroT"
                     /locus_tag="AC06IO_001075"
                     /gene_synonym="mavN"
     CDS             7658..9640
                     /gene="iroT"
                     /locus_tag="AC06IO_001075"
                     /gene_synonym="mavN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector ferrous iron transporter
                     IroT/MavN"
                     /protein_id="HIO9874278.1"
                     /translation="MNIKNIPIKPQKIPYYLFLLLLTVGASLILGFLSFGGMYALWPV
                     LPLAFAGFALSVAYEGEIYLQNIKGALNKLFKFNYLKNHFAKEYLLNHFPNTNEENCP
                     QFFKDYKRQLKLLAEFGHKELNQDSKKRKRQIEKTLSDMEKWFALQLFSDKDNPTPNT
                     SKYSRELLDWLALNDQDEWIKRWEKRHFQFHIVKGFSVVAGLFMGLGSTYLIVEAFTI
                     IPFFAAIPFTFWPIIILPMAIVAGAAYGMLTYNAVTDMINNNTVVKWYNKIRDDLSQG
                     LTVRNVFIASTAVFLVGLAVALTICTAGTWWTIATNARPLFDWMKKMPSFIMGIINPI
                     ITGASAIVFNIQNTAESLDMVDEATRSNKNVFQKIYEAISNGYQHLRETENWLQIANP
                     FRILLKLTITPLRILLFLGHLISVAVTSDRMPGVPQILSALVAIISEGFEDAHYFIGH
                     THEDEQDEEHGHQDHHQFEKLLKERLGPDSDQDHNMDIPTWILKTIASPIYGLAALWD
                     CSASKLNPPQENHSKNLPSGKKPHVLSLGEAWNKQWGVAKEFNVELSSHAKRPSQEWQ
                     VEHAVGQIDKFQRKHLKDIVIGHELADKKIIALNHLKEKIRHPEKGETLSDTLEQAKK
                     QPDYNQHRLFNQKGEKTRTQLFIEELPERINLSSGK"
     gene            complement(9724..11721)
                     /locus_tag="AC06IO_001076"
     CDS             complement(9724..11721)
                     /locus_tag="AC06IO_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIO9874279.1"
                     /translation="MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGY
                     NANSICAVTFTNKAANEMRARVSAFLPAASRRGLKVATFHTLGLSIIKRDVSRCDLKP
                     GFSIFDSEDCLQILRGFLPVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLL
                     CDEALTIYPRYQDALKAYNAVDFDDLIRLPVDLLNEHADVLEYWQNKIRHLLVDEYQD
                     SNTSQYLLVKKLAGVRAHFTVVGDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQN
                     YRSTSRILHAANHLIAHNQHLFEKKLWSDLGHGELLRVVSCKDEQDEAEHVIADLISH
                     KLRNRTKYSDYAILYRGNHQARIFEKVLRHHGIPYHISGGQSWFAKLEVRDIFAYLKL
                     LCNEADDAAFLRAITTPKRGIGESSLDALGRYAQSRGISLYHGSDHLALSEFVADKQR
                     MIFHDFKLWMEDIKKRISSDSVLEHLRQMVEDIGYEAYIYEQCDSPAKAQKKMDNVWE
                     LLEWVNRLLNKEPEQSLMDVVNKLILIDILEQSDESSSDVVQLMTLHASKGLEFPFIY
                     LVGMEEELLPHRVSIDDDQIEEERRLAYVGITRAQKGLCFTLAKQRRRAGELQDCVPS
                     RFLDELPQESLEWFGKGGERCEEQSKKLAKSHLEGLKNLLS"
     gene            complement(11780..11929)
                     /locus_tag="AC06IO_001077"
     CDS             complement(11780..11929)
                     /locus_tag="AC06IO_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874280.1"
                     /translation="MKNNSLLLPLFKTIDKLLFGIEEIKEIEKKPEYILCDNEDEFDV
                     ERSIN"
     gene            12139..12984
                     /gene="hslO"
                     /locus_tag="AC06IO_001078"
     CDS             12139..12984
                     /gene="hslO"
                     /locus_tag="AC06IO_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIO9874281.1"
                     /translation="MKEADTLQRFIFEHANIRGEIVHIEKTFQNIMNQRDYPPMVKNL
                     LGEALVSCLLLASSIKFEGNLSLQFQGDERLSLLLVQCDHNLNIRGFAKCAEGLEIAD
                     YATAFLQGQMVITISQDNQTQAYQSLIPIQSTSMSENLMTYFAQSEQIATRVWLAVNE
                     DMAAGMLLQLMPGQDTIQREQFWEYAVQLGQTVTEDELLTIDNQTLLYRLYHETELRI
                     FESRSTRFQCRCNQDKMKQVITVLGEEEATDLIKEKGKIEITCDFCNQKYTFDSIDVT
                     LLFRK"
     gene            complement(12998..13495)
                     /locus_tag="AC06IO_001079"
     CDS             complement(12998..13495)
                     /locus_tag="AC06IO_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874282.1"
                     /translation="MKQLILHPTDISQWHALVNEAQAATRLILNENTESYLVFLLMRF
                     SQGTKLIESVVALDFLESMRKPRQLQVELLRDVGDKSLLFCGLFPGIAKKRHVSLSYF
                     SDMGQAAYLTVGELEENQTANLYLQLSEQFITLQQVLQAMRGEYIHFSQSSCGVLADP
                     DLTMQ"
     gene            complement(13763..14722)
                     /locus_tag="AC06IO_001080"
     CDS             complement(13763..14722)
                     /locus_tag="AC06IO_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HIO9874283.1"
                     /translation="MSFKGFKVVMLILLSTQSYASKLASFSVCDSTIENPCIVQDSKT
                     QFSPVIRYREVASIADVYGGNITGINKFHLSGSEQPSEKGWEAIADSISRKMGAETKK
                     VIVLDLRQESHGYLNGRAITLVSVYNWINLGKPNSQSTLDQENWLAGLRSRKIVNGVL
                     TVPQYVAKQYSQGKSMVVSTVKNEEYYVYKKGFDYYRIFISDHRAPLDSEVDALVALI
                     KNNPEDTWYHVHCRGGKGRTTTVFAMFDMLKNADKVSFEEIIARQASIPPFYNLMVTN
                     REIPELTPYYEQRLQFLIHFYEFAHQSLMGYSGTWSEWKKLNI"
     gene            14897..15907
                     /locus_tag="AC06IO_001081"
     CDS             14897..15907
                     /locus_tag="AC06IO_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIO9874284.1"
                     /translation="MKYRKLGKIGPDVSALGFGCMGLSEFYGKPARLDDAVKIIRNAH
                     DQYQVNFFDTADIYGKGKNEELVGKAIKPFRNQVILATKCGVVRTGSDDEMSVNNSSA
                     YIKAACEKSLKRLNTDYIDLYYLHRYDHQTPIEEVMGIMHDLIDEGKIGYIGLSETDA
                     DTIRAAYTVVKDKLVAVQTEYSLRVRAPATAVLGTCRELGICFVAYCPIARGFLSGKI
                     KKPNLLGTNGFDFRTNVPQFQPENFDQNLGLVNELETIGEKTGYTPAQLSLAWLLAQG
                     EDIIPIPGTSNPQHLAENMRSIDIFLTPEQMTDLENAYKNNPVAGKRLSKELMAAFHL
                     KE"
     gene            16015..16827
                     /locus_tag="AC06IO_001082"
     CDS             16015..16827
                     /locus_tag="AC06IO_001082"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIO9874285.1"
                     /translation="MDYYFTKGKPQPVNMKTPIYLVTQFQQLMDLMQNQYEVSCLELM
                     QRSGKAACDFLVYRWPKVKKISIFCGRGDNGGQGYVLAQQAKKMGMIPTVWQVGHQMS
                     MSKPPQMHKEVWYEMNSCHQQGIVLHTYSPDIDLGDPELIVDALFGVGLYGHVRPEIA
                     LLLQRLQQFTVPILAIEVPTGINASTGEIAGNALAATATITFLCMKLGLLINDGKIYS
                     GEVAFDDLLAPEAIYQQVKGIEESNLLDSSTCFSKKIWYRNKTQKGWQLSIN"
     gene            16939..18765
                     /gene="typA"
                     /locus_tag="AC06IO_001083"
     CDS             16939..18765
                     /gene="typA"
                     /locus_tag="AC06IO_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIO9874286.1"
                     /translation="MIEKIRNIAIIAHVDHGKTTLVDKLLQQTGTLNERAPKVERVMD
                     SNALEKERGITILAKNTCVYWKGYQINIVDTPGHADFGGEVERILSMVDSVLLLVDAV
                     DGPMPQTRFVTQKAFARGLNPIVVINKIDRPGARPDWVMDQVFDLFDNLGANDTQLDF
                     PVVYTSALNGYAKLNLEDNATDMSALLQTIVDKVTPPCVDANGPFQMQISSLDYSSYV
                     GTIGIGRITRGQIKAKSPVKIIDKDGNMRSGRLLQLLGFKGLERVEVEEASAGDIIAI
                     TGIESLNISDTICDPNLVEALPALLVDEPTISMTFQVNDSPLAGQEGKYVTSRKIRER
                     LNTELLHNVALRVEDCDDPDKFRVSGRGELHLSILIETMRREGYELAISKPEVIIREV
                     DGEQQEPYEHLTVDVEESHQGTIMEKLGERRGEMQNMVPDGKGRVRLDYIIPTRGLIG
                     FHNEFLSCTSGTGLMYHVFDHYGPLIKGRIGKRLNGVMIANCPGTARAFALFNLQERG
                     RLFLEPQSVCYEGMIVGIHARDNDLVVNVTKEKQLTNIRASGSDENIILTPPVKLSLE
                     QALEFIDDDELVEVTPLSIRLRKKFLKEHDRKKASRTATNEE"
     gene            18772..19659
                     /locus_tag="AC06IO_001084"
     CDS             18772..19659
                     /locus_tag="AC06IO_001084"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HIO9874287.1"
                     /translation="MKRKFKRAILYARQHRANQEVNESLHRLVDFLSTQDIEIFQDSD
                     TAASFELKAPVLPREKMGAKHDLIIVVGGDGSLLSASRMAIKVNTPVIGINRGRLGFL
                     TDILPQDIESHLGPVLNGQYNEEERFLLHTKIYDKENSYFEGDALNDVVLGRGSETHL
                     IEFDVYINQQLVSHYRSDGMILSTPTGSTAYALSAGGPIMHPQLNAIVLVPMFSHSLS
                     SRPLVIDGEAEIELYISKSNETDLRISCDGHESRVVKPGQKVAVKKNGNRLRLLHPLD
                     YHYYDTLRSKLGWESKHQG"
     gene            19663..21330
                     /gene="recN"
                     /locus_tag="AC06IO_001085"
     CDS             19663..21330
                     /gene="recN"
                     /locus_tag="AC06IO_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIO9874288.1"
                     /translation="MLTTLRIEHFAIVKQLELDFTQGMTTFTGETGAGKSIMIDALLL
                     ALGEKADPSVVRPGEEKCDITAGFCFDESSEPAQWLAEHDISCHDGEIYLRRIIYAEG
                     RSKSYINGQPFPLQKIKELSEKLVHIHGQHQHQTLMQHATHRQQLDRYANNHLLLNDV
                     NTLYKQHQQIVQEVNALQSQEQQADRIKLLQFQIDELQALDIQDGELEALHQEHQILH
                     HAREYLQLSQQINELLNSDDQPNICSGLNQIFQLIQQLPQEQAQIKNTAELINSALIQ
                     CEEAINEMQQFADKIQLDPERLQEVEARISSIHQLARKYHLDSKSLQEHEKTLQNELE
                     SLQNVESKLAHLHLQLAQINKAYESAALKLRESRKIHAKKLAQEITASIQQLGMPKGW
                     IEIEITPLEKMQAHGLDKVEYKVCTNPGMEPDSLNKIASGGELSRISLAIQMITAQKG
                     STPTLLFDEVDVGIGGSTAALVGKMLRKLGERLQVFCVTHQPQVAASAHHHFLVTKHS
                     NNNQTFTHISLLKASDKIAEIARMMGGLTITEQTLSHAKELISLSNE"
     gene            21497..21703
                     /locus_tag="AC06IO_001086"
     CDS             21497..21703
                     /locus_tag="AC06IO_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIO9874289.1"
                     /translation="MSEKIRGTVKWFNESKGFGFLESGGKDYFVHFSAIQGSGFKTLA
                     EGATVMFKACNGQKGPQAEEVEVV"
     gene            complement(22042..23775)
                     /gene="ceg34"
                     /locus_tag="AC06IO_001087"
     CDS             complement(22042..23775)
                     /gene="ceg34"
                     /locus_tag="AC06IO_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg34"
                     /protein_id="HIO9874290.1"
                     /translation="MLMIMARVIVTYSPEVGGSSVLSLKESFRHVGSEVIDGDYRKMM
                     EDIPKEEFDKLYSTKEGRQRLFAHAKAKAMELLKNVDVLALSGNNSMIDPELFNQTRD
                     PDQNYDFSRTIAELALVHVATEKGMPILGVCGGHQVIAVYGGGEICDLNSDKLNTQKV
                     MNYDAIKVHKGSLIAQIIGGKQVQKEMDTARKAFNERMNQYNRLSEKINALEKLNLVQ
                     EAGKLDALGKLEELDKSEVQLLLKLTGLNIEEELFDLRELVGEIGETNQLKTELQNIK
                     TSLNGLEDSHSLTSPHYETEFFGAHEQVVSKLAAGFQHTGTASDNESNEVAESEFGVP
                     IITTQFHPEVGAKGLPEAHFFYLRTEAEQEMNLNIFRYLDKAGDAYNKKKNLMVELKQ
                     INPKNQDVLLQPSQIKKTSKELQQEREKQGNNSSSKRQTESYFFESILSKIGSYLSAI
                     GEFIREGIGKFLRDILSSMFTYDSVSRLKKKEQKQSKSEHSKIDLIDSKSNSYSTVFH
                     DMKVKSPSEILRENPAKLWAEKDDSHEKTITNQTDMDNSNKVRITTELVQEEVGQEFD
                     ENHISLYRNNL"
     gene            24188..25099
                     /locus_tag="AC06IO_001088"
     CDS             24188..25099
                     /locus_tag="AC06IO_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HIO9874291.1"
                     /translation="MTCKGSTVLMVPPEGFCFNYETSTSNSFQSFLSIKDIGNKALAE
                     FTAMVLQLEKEEIKVLTLSQSRDLPDAVFPNNWFSTHIDARGNNILIIYPLLAKNRQA
                     EVNIKGLMEVLNRAQFEVHEIIDLRNDKDEILEGTGSLVLDRENRLLYASLSPRTSLN
                     LVNKIADILGYTPIVFNSVDRQNKPVYHTNVIMGLSKCYAIICLDSINDAQQKAILSE
                     SFKETGKTMIDISWNQAQHMCGNVLELYNKKGESLLILSEQAKRHFTHEQLRIIQQHS
                     RLISTDIPTIEAIGGGSARCMMAEIFY"
     gene            25247..>26471
                     /locus_tag="AC06IO_001089"
     CDS             25247..>26471
                     /locus_tag="AC06IO_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2828 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874292.1"
                     /translation="MKISELKKLVAELNKEVSPKVTCTNIINLAGNLSEIIEYFEQDE
                     HVGPELIYRIEAVICEFWKLVSLTLPYEEWQSSIQVAPWLTLQQSLSKAGLLPTDFHH
                     PILYQRLKERYESFGHSELGIDQLLPLLIRCSRMTGYANKDPQSLDTYPHSPLNKQIE
                     ARRPQELAKLKDILCLLRAIFYLIHHCCTIEQLTLIPYLIYFRSPTTDEERRSELAIF
                     NWLTQKPADCLEFFKTNEDYIDTRSFRQISELAPLRPFIPTARSDFIKITNREHWIYP
                     FIQSRTNTSRSEYDLLNDAVNWLDTDFATEKDKSYHAALEFAHTVKKQANILTQREMK
                     IVHSALYVFCLDKYVKHRKADPRPRCTPFSLSGETKCQAAEKKQQEILGKPTKFGFFE
                     NLALNEGRLKTLTKTF"
BASE COUNT         8543 a         5253 c         4912 g         7763 t
ORIGIN      
        1 ttgcttttat acaggaaaga gtaaaaaaag aatcacatta tatccttctc agggttaagt
       61 aaaaggggat ggcgcatgaa tacacaagat tttttagcgg gttataccaa acgatttgtt
      121 gataacaaag aagaagacat gagtttggat gaatatctgg agttgtgcaa aacggacccg
      181 acagcatatg ccaacccagc agaacgtttg cttatggcga taggcgagcc tgaattgatc
      241 gacacccgac atgatccggt tctatcacga attttctcaa ataaaataat ccatcattat
      301 gaagttttta aagattttta tggaatggaa gaacccattg agcaaatcgt tggctttttg
      361 aagcatgctg cgcaagggtt agaggaaaca aaacaggttt tatatctttt ggggcctgtt
      421 ggcggtggta aatcttccat tgcagaaaaa ttgaaagatt taatggaaag aatccctttt
      481 tatgcaatca aaggttctcc tgtctttgaa tctccactgt cattatttaa ccccttggaa
      541 gatggtgaat tacttagaga acgttttggt atcccaacac gctacctacg ctacttaatg
      601 tccccctggg cagtcaagcg attacaggaa tacaatggcg acatcagcaa attcagagtt
      661 attaaagtca agccttctcg actcaaacaa attgccatcg caaaaacaga acctggcgat
      721 gaaaacaatc aagacatttc ttctctggtt ggtaaagtcg atatccgtaa attggaagaa
      781 ttttcccaag atgatcctga tgcttacagc tactctggag gcttgtgtcg agccaaccgc
      841 gggcttttgg agttcgttga aatgtttaag gcaccgataa aggtccttca tcccttgctg
      901 actgcaacgc aagaaggaaa ttacaatgcc acagaagggc tttcagctat tccttttgaa
      961 ggaattatac tggcccactc caatgaatca gaatggcaat catttagaaa caataaaaat
     1021 aacgaagcat ttatagaccg aatcaacata gtcaaagtgc cttattgcct gcgtgtttct
     1081 gaagaaatca aaatctacca aaaactaatc cataacagtt ctttagccaa tgccccttgc
     1141 gcacctggta cactggatat gctagcccag ttttctgtat taacccgttt aaaagaacca
     1201 caaaactcca gtatttactc caaaatgcgc gtgtataatg gggagagttt gaaagatacc
     1261 gatcccaagg caaaatcgta tcaggagtat agagattttg caggggttga cgaagggatg
     1321 agcggaatat ccacgcggtt tgccttcaaa atcctctcaa aagtattcaa ttttgatcat
     1381 actgaagtgg cagctaaccc ggttcatttg atgtatgtgc tggaacgcca aatcgaacag
     1441 gaacaatttc ctcaagagtt acatgaaacc tacttgaact tcattaaaga atatttggcg
     1501 accaaatacg tcgattttat aggcaaggaa attcaaaccg cttacctgga atcctattct
     1561 gagtatgggc agaacatatt tgaccgttac attacctatg ctgatttctg gattcaagat
     1621 caggattatc gcgatccgga tactggcgag atatttgaca gagccttatt aaacctggaa
     1681 ctggagaaaa tcgaaaaacc ggcagggatt tccaatccta aagacttccg taatgaagta
     1741 gtcaatttcg tactgcgcgc tcgagcaaat aaccgaggaa aaaaccctgt atggaatagc
     1801 tatgaaaaac taaaatcagt gattgagaaa aaaatgttta ccaatacgga agatttgtta
     1861 cccgttattt ctttcaatgc caaagcatct gaggaagata agaaaaaaca cgaagagttt
     1921 atctctcgaa tggtagaaaa aggctacaca cgaaaacaag ttcgcttact ttgtgaatgg
     1981 tatttgagag taaggaagtc acaataatag gtaggatcat tcataactca ataggttatg
     2041 aaatacagtc agggttaata agtaactatc tgaaaagatc aagtagaatc ctaaattcag
     2101 atactgctta caacctggct aacaaaaaac tgacctgcag aaggtgtgga gctaacaatt
     2161 atgtcgcaat taattgatag acgacaaaat gctggaaaaa aaagcacggt taatcgccaa
     2221 cgttttttac gtcgctataa aagccaaatt aaaaaagcag tttctgaggc tgtcggaaaa
     2281 cggagtatca ctgaaattga tcaaggcgaa caaattacca taccagctaa agacatttat
     2341 gaacctcaat tccatagggg ccatggaggc catatcgaac gtgtactacc gggaaatgat
     2401 aatttcatcg caggggatcg gataaaaaga cccggcggcg gtggttctgg aggagctgga
     2461 ggtaacgcca gcgatagtgg tgaaggcgaa gataattttg tttttgaatt atctcgagaa
     2521 gagtttttgg aactctattt tgaagatctg gaattacccg atttggtaaa aaaagaacta
     2581 gcaagaatta gcacttataa aacagtaaga gctggtgtaa ccaccagtgg aatacctaac
     2641 aacattaatg tccttcgctc aatgaaacaa gcgacaggac gccgtgtcgc tttagcctct
     2701 ccttataaaa gacgattaaa ggaagcggaa gaagagcttg aacgccttaa gcaattagcc
     2761 aatccagaca aactagacat tttgaagctt gaaagagaca ttgagttttt caaaaaaaag
     2821 atacaaacag taccttttat agacacaatt gatctgcgtt acaatcaccg tgtgcgagta
     2881 ccatcaccat ctacacaggc agtgatgttt tgtgtaatgg acgtatcagg ctccatggat
     2941 gaagccaaaa aggacatagc gaaacgcttt ttcatcttac tctatatgtt tcttaccaaa
     3001 aactacgaaa aaattgagtt ggtatttatc cgccatcaca cttcagccaa agaagttaat
     3061 gaagaggaat ttttttattc gcgagagact ggcggaactg ttgtttccag tgctctggag
     3121 ttattaaaca ccatcattga ggcacgctac cctcctcaag cctggaatat ttatgttgcc
     3181 caagcatcag atggcgataa ctggaatgcc gactctccct attgtcagga gttgctgcaa
     3241 gaaaaaatta tgcctttatt gcaatatttt gcctatattg aaattatgcc ccgtcatcat
     3301 caaagtttgt gggaagtata tcaacaagta aaagaacgtt atcctaattt tgccatggaa
     3361 aatattgata atgttgccga tatttatccg gtatttcgcg aattatttaa aaggaaaacg
     3421 gtatgagaaa aaagcctcta tcaactggtg cagaatggac tttcgagtta atacaggcct
     3481 atgatcgcga aattgcacgc atcgccaaaa gctttaattt ggatacctat ccaaaccaaa
     3541 ttgaaatcat taccgctgaa caaatgatgg atgcctattc ttcggtaggt atgcctatcg
     3601 gttaccacca ttggtctttt ggaaaacact ttgtcagtgt tgagaaaagc tacaaaagag
     3661 gacaaatggg cttggcctat gagcttgtaa ttaattccaa tccctgtatt tcttatttaa
     3721 tggaagaaaa taccatggct atgcaagcct tggttatcgc tcacgcatgc tatggccaca
     3781 attctttttt taagaacaat tacttattta aaatgtggac ttctgcggac gctattattg
     3841 actatctggt attcgccaag aaatacatca gcgattgtga ggaacgctac ggcatagatg
     3901 cggtagaacc agtgcttgat gcttgccatg cccttatgaa ttacggggtt gatcggtaca
     3961 aacatcccac acccttatct atccaggaag aaaaaattcg acagcaaaac cgtgaaatct
     4021 acatgcaatc acaagttaat gagctatgga gaacgatacc tttcagtaag caaatcgata
     4081 aaaacggaca aaaaaaacgc tttccagaag agcctcagga aaatattctc tattttatag
     4141 aaaaaaatgc ccctttatta gagccttggc agagagaaat agtgcgtata gtaagaaaac
     4201 tggcccaata tttttatcct cagggacaaa ctaaagtgat gaatgaaggg tgggcctgct
     4261 tttggcatta tactattttg catgctttgt atgacgaagg tttagtgact gatgaattta
     4321 tgctggaaat actgcaaagt cataccaatg tcatcatgca gccagcctat aatagccctt
     4381 attacaatgg tattaatcca tacacccttg gctaccatat gatgcaagac atcaaacgaa
     4441 tttgtgaaaa cccaacagac gaagacaaaa agtggtttcc ctatctcgcg aataccgatt
     4501 ggctcacaag ccttgatacg gcgatgcgca actacaaaga tgaaagtttt atcgctcaat
     4561 acctgtcacc aaaacttata cgagatttaa aactgttcca tattgttgat gacgaccgaa
     4621 actctgaatt atacatcaat gccattcacg acgaatgggg atatcagcaa atcagagaag
     4681 cattatccag acaatacaat ctgggttatc tggagcctga tattcaagtc tactccgttg
     4741 atttgcaagg agatcgttct ttgactttaa ggtactccca acaaaacaga gtaccacttg
     4801 gaagctcaac gaatgaagta ttaaagcact tgcattattt atggcaattt ccagtcatat
     4861 tacaggcagt tgatggagaa aataaaatca ccgaggaatt tagttgccca cctcttaata
     4921 aaaataaaac agcggaaact aattgatagt cttatattac tggctcaaaa tagctattag
     4981 ttttctatta taactaagag ccagtatcta ttctttttct tctccttaat tcactgccct
     5041 taatatgctg ctctaatgac tgctcctgat gtaatcttga agcaacgaaa caatttaatc
     5101 tggctactcc ttagacaata cctttcattg aggcaatacg tacttatttg gacatagctt
     5161 cggcaacagc aatattgagg caacgatgca atttacatac ttatttgatg atggcttcgg
     5221 caacaggtaa tttgaggcaa ttcggttgtg gcaatagaaa tactctccta ataatttctt
     5281 aataattttg tactataatt tgccataacc aatctttgga gtcactgcca tgcctttaac
     5341 tcaaacccaa cgcctgatta atacctacgg cgcttcactt aaaaatggaa caatctctaa
     5401 cgaagaactg attattttgc tggatccaaa cacttttaca aaatcagaag gttatgttga
     5461 tcccaatgcc ccggtttcag actccaatca tagtaaaatg gatgcaataa aggattttgt
     5521 attaaccatc ggctcgactt tagattctga aatacttcac caattaacat ctcgaatgat
     5581 cgaattatcg ccccccggag atagaaacac atttatgaga ggttctagct tggaaaaagc
     5641 tttcttagct tttgaaatgg ctcactaccc aacaaaagca gaggagcatt tcaactcgac
     5701 aagagtgaga acagaattcc caggcgaaaa tgatatagac aatcttaaag cagttatttt
     5761 gaacccaatc attgcatttt tccaatctcc tcccggaacc gatgaaaaat tagaaaaact
     5821 tatcaaatta tgcacggatt ataaagagca cttgaaaccc tctctaaccg acgacgctaa
     5881 taatttagta aataaaaaac atgcgattgt tacaaacatg ctagccgctc tcactgacag
     5941 cacagcccca aatagcgcaa caaaaattaa aaatatggag caaatactga ctgaagaaaa
     6001 caaagaaacc ttaaaagatc gccgagattc atttagcggt gctagatttc tagaaagtat
     6061 cctgcacata ttgagtctgg gtatttattc aaaactgacc aaaggcactt ttaaattttg
     6121 gaaaagccat ggcgaggctc tctccgataa cattgaagaa gttagtaaac agcagcctgg
     6181 catgaaataa atatcaattg atatcaataa attaacttta aaaaatagcc tctcaaaaat
     6241 atttaaaatt tatgagaggc tttaaaacag ttactcagaa gcgagttcaa tagcgaaagc
     6301 taccgcgagt ttggagaaat tggtcatatg ttccaggttc aataaatcca ttttatcaga
     6361 agaagtatgg atgtatgggt tgtgatcagc gaaactggtt tcacatggaa atgcagcagg
     6421 aatatcttct tcattccagg aagcatgatc actgcaacca taaccacatc gtgaataatc
     6481 tactggcaca tgaatataat gatcaattaa tttggccaaa taattactta aatctctatc
     6541 tgtgtaatca gtaaataccc acatagttgg atcattggca tcatttcgat aaccggtcat
     6601 gtcaaattgt accacagcct tcactggaat tgattgttcc tgaaagtgtt ggactacgtg
     6661 ttgtgaaccg accaaacctc tttcttcagc agcataccaa ataaaataaa tggggcgctt
     6721 aaatgttgtt ttagaagaca aaataacccg tgcggcttcc ataatgctcg atgatccact
     6781 tccatcatca ccagctccag gcatacggcc atcaagagta tccatatgag caccaataac
     6841 aatagcaggg gctttgatgt ctttgccaat cacggtaacc agggaaggtt gtttatacca
     6901 ccctgttttt acaaaaaagg tggaggtatc agttcgtcca tactcaacgg ccatctgttc
     6961 aaatttggat tttaaccaat tggcagtttc aacacccgta tcttttgttg cagagcggtt
     7021 ataataggaa gtcatatgag tcagagtttg ccatatgtta tcagagacaa tttcttttag
     7081 tgccgcattg acttcttctt catgcttgat ttcataaacg tctttatgta atttggatac
     7141 accaaacggc ttaacgagct ttttttgcag taacttttga gcagattgct gttggtttgc
     7201 cgctaaggtt cccgttaatt tatgactcac attcacaaaa tggccgcaac tgactttatc
     7261 agccagaata gtcaaggtct ctacatcaga tgaaagtaca tcaataattt tgaattcttt
     7321 attctcggcc aaaatcttat ggggtacggt tatttttgca gcaaggcatt gtgggacttg
     7381 caattgctca tgaacaggcg acgtcgttgc aaaacttgaa ttactggcga gtattaatcc
     7441 tgatgtgata caggtcatcc attggttaaa acgcatatcc tctcctaagt tatttaaatc
     7501 gtttttgata ctacctgatg aaacgaatat tgcaaacaaa ctttattgtt gaattgaaac
     7561 aaagacaaaa aatacttttt taatattaat gctattgata atcattttca tttggtataa
     7621 agttctgcat tacgcatcaa tgagcaaata tactacaatg aatataaaaa atatacctat
     7681 taagccccaa aaaatccctt attacttgtt tcttttgtta ttaactgttg gagcaagcct
     7741 gatactcggc tttttaagct ttggaggcat gtatgctctt tggcctgtgt tacccttggc
     7801 ttttgctggt tttgcgttat cggtagctta tgagggtgaa atttatttgc aaaacataaa
     7861 aggcgctctt aataagcttt ttaaatttaa ctatcttaag aatcactttg cgaaagagta
     7921 tttgctaaat cattttccaa acactaacga agaaaattgc ccgcaatttt tcaaagatta
     7981 taaaagacag ttaaaattat tggctgaatt tgggcacaaa gaacttaatc aagacagcaa
     8041 aaaaagaaag cggcaaattg aaaagacact ctcagacatg gaaaagtggt ttgccctgca
     8101 attgttttca gataaagaca atccaactcc aaatacatcc aaatactcca gggaactact
     8161 tgactggctt gctttaaatg atcaagacga atggataaaa cgatgggaaa agcgtcattt
     8221 tcaatttcat attgtcaaag gatttagcgt ggttgccggc cttttcatgg ggcttggaag
     8281 cacctatctt attgttgaag cctttacaat aattccgttt tttgccgcca ttccatttac
     8341 attttggccc attatcatcc ttcccatggc tatcgtcgca ggagccgctt atggaatgct
     8401 gacctacaat gccgttaccg acatgatcaa caataacacg gttgtcaaat ggtataataa
     8461 aattcgcgat gacttaagcc aaggtttaac tgtacgcaat gtatttatag catccaccgc
     8521 tgtttttctt gttggactag cagtagccct aaccatctgt actgctggaa catggtggac
     8581 gattgctaca aatgcccgcc ctttatttga ctggatgaaa aaaatgccaa gttttattat
     8641 gggaattatc aatcccatta taactggcgc ctctgctata gtctttaaca tacaaaacac
     8701 ggctgaatca ctggatatgg tcgatgaagc aactcgtagt aataagaacg tcttccaaaa
     8761 aatttacgaa gcaataagca atggatatca gcacttacgt gaaactgaaa attggttaca
     8821 aatagctaac ccgtttcgca ttcttttaaa attaaccatt accccattgc gtattctttt
     8881 gtttctgggg catttgatca gcgttgcagt cacatcggat cgtatgcctg gagtaccgca
     8941 gattctttcc gcactcgttg ccattattag cgaaggcttt gaagatgctc actattttat
     9001 tggacatacc catgaagacg aacaggatga agaacatgga caccaagatc atcaccaatt
     9061 tgaaaaactc ctaaaggaaa ggctgggtcc tgactccgat caagatcata atatggatat
     9121 tcctacctgg atattaaaaa ccattgcttc acctatatat ggcttggcgg cactctggga
     9181 ttgttctgcc agtaagctta atcctcctca agagaatcat agtaagaatt tgccttctgg
     9241 caaaaaacct catgtgttaa gtcttggaga agcatggaat aaacaatggg gagtagccaa
     9301 agagttcaat gttgaattaa gtagtcatgc caaacgccca tcgcaagaat ggcaagtgga
     9361 acatgctgtt ggtcaaatcg acaaatttca aagaaagcat cttaaagaca tcgttatcgg
     9421 tcacgaatta gccgataaaa aaatcatcgc cctcaatcat ttaaaagaaa aaatacgcca
     9481 tcctgaaaaa ggagaaacat tgagtgacac tctggagcag gctaaaaagc aaccagacta
     9541 caatcaacac agactattta accaaaaagg ggaaaaaacc cgtacacaac ttttcattga
     9601 agaactccca gaacgaatca atctctcttc tggcaaatga cagaggttaa cattagtacc
     9661 ttaaaaagca gtaaggagtt cagttaatga tactccttac tggcgataaa atataatgta
     9721 ctcttaactc aacaaatttt tcagtccttc caggtgggat ttggccagtt tttttgattg
     9781 ttcttcacaa cgctctccgc ctttaccgaa ccattccagg ctctcttgcg gcaattcatc
     9841 caaaaatcgg cttggaacac aatcttgcag ttctccagct cgacgtctct gcttcgctaa
     9901 agtaaaacat aatcccttct gggcacgtgt gatccctaca taagccaatc ttctttcttc
     9961 ctcaatttgg tcatcatcaa tactcactcg gtggggtaat aattcttctt ccatcccgac
    10021 caaataaata aaaggaaatt ccaaaccttt tgaggcatgc aatgtcatta attggactac
    10081 atcacttgaa gattcatcgg attgttccag tatatcgatt agtattaatt tattcacaac
    10141 atccatgagc gattgttcag gttccttatt caacaaacga ttaacccatt ctaaaagctc
    10201 ccatacatta tccatttttt tttgtgcctt ggcgggagaa tcacattgtt cataaatata
    10261 agcttcatac ccaatatctt ccaccatttg ccttaaatgc tccagaactg aatctgagct
    10321 gatacgtttt ttaatgtctt ccatccacaa tttgaaatca tgaaaaatca ttctctgttt
    10381 gtcagcaaca aactcagaca aggccaaatg atcggacccg tgatataaac ttatccctct
    10441 ggactgagca tagcgtccca aagcatcgag actgctctct cctatacctc gttttggggt
    10501 agtaattgct cgcagaaaag cggcatcatc tgcttcatta caaagtaatt taagataggc
    10561 aaaaatgtcc ctgacctcta acttggcaaa ccaggactgg cctccgctga tatgataagg
    10621 aataccgtga tgtcttaata ccttttcaaa tattcttgct tgatgatttc ctctgtataa
    10681 gatggcgtaa tcgctatatt ttgtccgatt acgtaattta tggcttatta aatcagcgat
    10741 cacatgttcc gcctcatcct gctcatcctt acagctgaca actcttaata attccccatg
    10801 ccccaaatca ctccataatt ttttttcaaa taaatgttga ttatgagcga tcagatggtt
    10861 ggcagcatgt aaaattcggc tggttgatct gtaattctgt tctaatttaa taatctttaa
    10921 ccgaggaaaa tctttttgta actgcgccaa attctctggt ttggcaccac gccaggcata
    10981 aatcgattga tcatcatcac ccacaaccgt aaagtgggca cgaacccctg ccaatttttt
    11041 taccagaaga tactggctag tgtttgaatc ctgatattca tccaccaaca aatgacgaat
    11101 tttattctgc caatattcca acacatcagc atgctcattc agtagatcga caggtaaccg
    11161 aattaaatca tcaaagtcca ctgcattata tgcttttaat gcatcttgat agcgtggata
    11221 aattgttaac gcctcatcac ataaaagtgt ttccggaggg ttttggagta ttaattcggg
    11281 attcaataaa ttatttttcc aaagagaaat ttgttgttga actttgagga taaaatctct
    11341 atcggttgcc ttattaacag gtaaaaaacc ccgcaaaatt tgcaaacaat cttcactatc
    11401 aaaaatggaa aatcccggtt tcaaatcgca cctagaaaca tcacgcttga tgatgcttag
    11461 gcctaatgta tgaaatgtcg caacctttaa tccccgcctg cttgcggctg gtaaaaaggc
    11521 cgaaaccctt gcgcgcattt cattcgccgc tttattcgtg aaagtaacgg cgcagatgga
    11581 gttggcatta taaccacatg aattaattaa ataaccaatt ttttgtgtaa taactctggt
    11641 tttaccactc ccagccccgg caagcaccaa caaaggccca tcaatatatt ttactgcagc
    11701 catttgttgg ctattcaaca tacttattcc cgctcaaaaa aaaggcatta tcacccaggc
    11761 tttctttttc acgcaagaat taattgatag agcgttctac atcaaactcg tcttcgttgt
    11821 cacagagaat atattctggt tttttttcta tttctttaat ttcttctata ccaaacagta
    11881 atttgtctat tgttttaaat aggggaagaa gtaaactgtt atttttcata ataactcctt
    11941 acaattttaa aaaggggttt tttaagaata gtccacgaaa taaatttctc caggaaaatt
    12001 aaaaaataat taagatacgc tggttatgga aactactatt cctcttgtaa gattttgttc
    12061 aaaataaaac tgaactcaga aaaggcctga gataaacatt ttatgttaga atagtcctat
    12121 ttgtgttatg agtttgaaat gaaagaagca gataccttac agcgttttat ttttgagcat
    12181 gcgaatatcc gtggcgaaat cgttcatatt gaaaaaacat ttcaaaacat catgaaccaa
    12241 cgtgattacc cacccatggt aaaaaatctt ttaggtgaag cgttagtctc atgtttactc
    12301 ttggcaagca gcattaaatt tgaaggaaat ttaagtctgc aatttcaagg tgacgaaaga
    12361 ttatccctgt tactcgttca atgcgatcat aatttaaata taagaggctt tgcaaaatgt
    12421 gcagagggtc ttgaaattgc tgattacgcg accgcttttc ttcaaggcca aatggtcatt
    12481 acgatcagtc aagacaacca aactcaagcc tatcaaagcc tgatacctat tcaatccacg
    12541 tccatgagcg aaaatctgat gacttatttt gcccaatcag aacaaattgc tactcgtgtt
    12601 tggttagctg tcaatgaaga tatggctgca ggtatgctat tacaacttat gccaggccaa
    12661 gatacaattc aaagagaaca attttgggaa tatgccgttc agctggggca aacagtaact
    12721 gaagatgaat tgctgaccat agacaatcaa acactactct atcgccttta tcatgaaacg
    12781 gaattaagaa tatttgaaag ccgctctacc cgtttccaat gccgttgtaa tcaggacaaa
    12841 atgaaacagg tcattactgt cctgggagag gaagaagcaa cagatttaat caaagaaaaa
    12901 ggaaaaatag aaatcacttg cgatttttgc aatcaaaaat acacttttga ttcaatcgat
    12961 gtgactctgc tatttcgcaa ataaaatgag catctgccta ctgcatagtc aaatcaggat
    13021 ccgccagaac gccacaactt gactgactaa aatgaatata ctcccctcgc atggcttgca
    13081 gtacttgctg caaagtaata aattgctcgc ttaattgcaa atacaaattg gctgtctgat
    13141 tctcctcaag ctctccgaca gtcaaataag ccgcttgccc catgtcagaa aaataactca
    13201 aactcacatg gcgtttttta gcgatgcctg gaaataagcc gcaaaacaat aagcttttgt
    13261 cccccacatc ccttaataat tcgacttgta gctggcgagg tttgcgcatg gattcaagaa
    13321 aatcaagtgc aacaacggat tcgataagct ttgtgccttg agaaaagcgc atcagcaaaa
    13381 aaaccaaata actttccgtg ttttcattca atatcagtct ggtagctgct tgcgcttcat
    13441 taaccagcgc atgccattgg ctaatatcag tgggatgtaa tattagttgt ttcataaata
    13501 ccccccatat ttacctctta ctattagtct agcaaacaaa taccaattgt tttctatgac
    13561 taatataata attcgtaatg catttttatg atgaaatcaa ggatttctta tcttggggca
    13621 gataaatcct aagaaaacca acacttaatt taagattgat atggtatttg ccaaaaacaa
    13681 ttgagtgcca agccttcatg aaattcgctt catttcaaat gaaggctttt taatatgatc
    13741 ttcttgcaaa tggcactaga tgctagatat tgagcttttt ccattctgac caggtacctg
    13801 aatatcccat gagggattgg tgagcaaatt cataaaaatg aatcaaaaat tgcagccttt
    13861 gctcataata aggagttaac tcagggattt cacgattcgt caccatcaaa ttataaaaag
    13921 gcggaatgga ggcttggcgt gcgatgattt cttcaaaact aaccttgtct gcattcttta
    13981 acatatcaaa catggcaaaa acggtcgtcg tgcggccttt tccgccccgg cagtggacat
    14041 ggtaccatgt gtcttccgga ttatttttta tcagtgcaac aagcgcatcc acttcagaat
    14101 ctaaaggagc acggtgatcg gatatgaata tgcgataata atcaaatcct tttttatata
    14161 cataatattc ttcatttttt acagtgctca ctaccatcga tttgccttga gaatattgtt
    14221 tagctacata ttggggcaca gttaaaacac cgttaacaat ttttctggat cttaagccgg
    14281 ctaaccaatt ttcctggtcc aaagtactct gactattggg cttgcccaaa tttatccagt
    14341 tatagacgct aactaaagta atcgctctgc catttagata accatgactc tcctggcgaa
    14401 gatcgagcac tataactttt tttgtttcag cccccatttt tctggaaatg gaatcggcaa
    14461 tagcctccca acccttttcg cttggttgct cgcttcctga caaatgaaat ttattaatcc
    14521 ctgtgatatt gccaccgtaa acatccgcaa ttgatgcaac ctctctatat ctaatgacgg
    14581 gtgaaaattg ggttttacta tcctgaacaa tacatggatt ttcaatagtt gaatcacaaa
    14641 cactgaagga tgccaattta gaggcataac tttgagttga taataatatc agcattacca
    14701 ctttaaatcc tttaaagctc ataataaatc ctttttaaat ccctaaagat gaaccgcagc
    14761 attctatgta aaagattttt attattcaag acgcgttacc atttcagaaa tgaaatcaca
    14821 atccttaata ttttcctgcc aaatcattct atacttgatt gaacagctta aatcaaacca
    14881 tgtccgggga tagaccatga aatatcgcaa attggggaag attggtcctg atgtctcagc
    14941 attgggcttt ggatgcatgg gattatcaga gttttatggc aaacctgctc gccttgatga
    15001 tgcagtcaaa attattcgta atgcgcatga ccaataccag gttaatttct ttgatacagc
    15061 tgatatttat ggaaaaggta aaaatgaaga attagttggt aaagcgataa aacccttccg
    15121 taaccaggtt atacttgcaa cgaaatgcgg agtagtaagg acaggttctg atgatgaaat
    15181 gagtgtcaat aattcatctg cctatatcaa agcagcctgc gagaaaagcc taaagcgctt
    15241 aaataccgac tacattgatc tttactattt acatcgttat gaccatcaaa caccaattga
    15301 agaagtgatg gggataatgc atgaccttat tgatgaaggc aaaattggtt atattggatt
    15361 atctgaaact gatgctgata ccattcgggc agcttataca gtagtcaagg acaaattggt
    15421 tgctgttcaa actgaatatt cacttcgagt tcgagctccg gcgacagcgg ttttgggcac
    15481 ctgccgtgaa ctgggtattt gttttgttgc ctactgcccc atagcacgtg gtttcctcag
    15541 tggaaaaatt aagaagccca atcttttagg gacaaatggc tttgatttca gaactaatgt
    15601 tccgcaattc caacctgaaa actttgatca aaatctcggc ttagtcaatg aacttgagac
    15661 cataggagaa aaaacaggtt acaccccggc acaattatca ttagcttggc tattggctca
    15721 aggagaagac attatcccca ttcccggaac ctcgaatcca caacacttag cagaaaatat
    15781 gcgctcaatt gatatttttc taactcctga acaaatgaca gatttggaaa atgcttataa
    15841 aaataatcct gttgccggaa aacgattatc caaggaactg atggcagcct ttcatttaaa
    15901 agaataagtt acatttgaat agcagaggag aatgacgctt tcttaaccac aaatcaggaa
    15961 actctccaac actctaaaaa ccatgtttac aaaagttaga aaagattttt ttatatggac
    16021 tattatttta ctaagggaaa accacagccg gtcaacatga aaacgcctat ttatcttgtt
    16081 acacaatttc aacaactgat ggacttaatg caaaaccaat atgaagtttc ttgtctcgaa
    16141 cttatgcagc gttcgggcaa agcagcctgc gactttttgg tgtatcgttg gccgaaagtt
    16201 aaaaaaatca gtattttttg tggtcgtggc gataacggag gacaaggcta tgttttagct
    16261 cagcaggcaa aaaaaatggg aatgatccca acagtttggc aagtaggaca ccagatgagt
    16321 atgtcaaaac cgcctcaaat gcataaagaa gtatggtacg aaatgaattc atgccatcag
    16381 caaggtattg ttctacatac ttattcgcct gacattgatt taggcgatcc cgaattgatt
    16441 gtcgatgccc tttttggcgt agggttgtat ggtcatgttc gcccggaaat agctttgctt
    16501 cttcaacgcc tccaacagtt cactgtaccc atcctggcta tcgaagtgcc tacaggcatc
    16561 aatgccagca caggtgaaat agcaggtaat gccttggctg caaccgccac cattaccttc
    16621 ttgtgtatga aattaggctt attaataaat gatggaaaaa tttatagtgg agaagtagca
    16681 ttcgatgatt tactggcacc tgaagcaatt tatcaacaag tcaaaggcat agaagagtca
    16741 aacttgcttg atagttctac ttgcttctcc aaaaaaattt ggtatcgcaa taaaactcaa
    16801 aaaggctggc agttgagcat caattaaaga gtagatattc tttaatgagc gattagtgta
    16861 taatgttaaa tctatgaaca attgtcagtt attatcttaa attattaaat ctcgctattc
    16921 cccaactaaa ggttttttat gattgaaaaa attcgcaata ttgccattat tgcccacgtt
    16981 gaccatggca aaactactct tgttgacaag ctcttgcaac aaactggaac gctcaatgag
    17041 agagctccaa aagttgaacg cgtgatggac tccaatgcac ttgaaaagga acgtggaatt
    17101 actatcttag ccaaaaacac ttgcgtttat tggaaaggtt atcaaattaa tattgtagac
    17161 accccagggc atgcggattt tggcggcgaa gtggaacgta ttctttctat ggttgacagc
    17221 gttttattgc tggtcgatgc agttgacggc cccatgccac aaacccgctt tgtcacccag
    17281 aaagcatttg cccgtggctt aaaccctata gtcgtgatca ataaaattga ccgtccagga
    17341 gcaagacctg attgggtcat ggatcaggtg ttcgacttgt ttgataactt aggcgccaat
    17401 gacacccaac tagactttcc tgtagtttat acctccgctt taaatggcta tgccaaactt
    17461 aatttggaag acaatgcaac agatatgtct gccctgctgc aaacaattgt agataaagtc
    17521 acaccacctt gcgtcgatgc caatggtccg tttcaaatgc aaatcagctc tctcgattat
    17581 tcttcttatg ttggcaccat aggcattggc cggattacac gcgggcaaat taaggcgaaa
    17641 tctcctgtaa aaattattga taaagacggt aatatgcgca gcggacgact gctccagtta
    17701 ctcggattca aagggctgga acgcgttgaa gtcgaggaag ccagtgccgg tgatattatc
    17761 gcgataaccg gaattgaaag cctgaatata tccgacacga tttgcgaccc caacctagtg
    17821 gaggcattgc cagcactttt ggtagatgaa cctacaatca gcatgacttt tcaggttaat
    17881 gactcgcctc tggccggcca agaaggtaaa tacgtcacat caagaaaaat tcgtgaacgc
    17941 ttaaatacag aattactgca taatgtggcg cttcgcgtag aggactgcga tgaccctgac
    18001 aaattcagag tttctggtcg cggggaatta cacttgtcga ttcttatcga aacgatgcgc
    18061 cgtgaaggct atgaattggc aataagcaaa ccggaagtaa ttattcgcga ggtagatggt
    18121 gaacaacagg aaccttatga acacttgaca gttgatgttg aggaaagcca tcaaggtacc
    18181 atcatggaaa aattgggtga acgccgaggc gaaatgcaaa atatggttcc tgatggcaaa
    18241 ggcagagtac gtttggatta catcatcccg actcgcggat taatcggttt tcacaatgaa
    18301 tttttatcct gcacctcagg taccggatta atgtatcatg tctttgatca ctacggcccg
    18361 ttgattaaag gacgcatagg gaagcgtctt aatggcgtca tgatagctaa ttgtcctggt
    18421 actgcccggg cttttgcctt gttcaattta caagaacgtg gaagattatt tttagagcct
    18481 caatcagtct gctatgaagg aatgattgta ggtatccatg ccagagataa cgacctggtt
    18541 gttaatgtca ccaaagaaaa gcaattaaca aacatcagag catcaggttc ggatgaaaat
    18601 atcatcctga ctcctccagt caaattatct cttgagcaag cgctggaatt tatcgatgat
    18661 gatgagttag ttgaggttac cccgttatct attcgtttac gtaaaaaatt tctcaaggaa
    18721 cacgatagaa aaaaagcctc tcgtactgcc acaaatgaag aataatacat tatgaaacga
    18781 aaatttaaac gagccatctt gtatgcccgt caacatagag caaatcagga agttaatgaa
    18841 agcttgcatc gactggttga ctttttaagc acacaagata tagaaatctt tcaggactca
    18901 gatactgcgg ccagttttga attaaaagcc cctgttttac ctagagaaaa aatgggggca
    18961 aaacacgact tgattattgt cgtgggtggt gatggcagtt tactttctgc ctcacgtatg
    19021 gctataaaag ttaatacacc ggtcataggg attaacaggg ggaggctagg ttttttaacg
    19081 gatattttac cccaagacat tgagtcccat ttaggccctg tgcttaatgg acaatataat
    19141 gaagaagaac gcttcttatt acatactaaa atatatgata aggaaaacag ttattttgaa
    19201 ggcgatgcac tcaatgatgt cgtattaggc agaggaagtg aaacgcattt gatagaattt
    19261 gatgtttaca tcaatcagca gctggttagc cattaccgtt cagatggaat gattttatcc
    19321 actccaactg gctcaacagc ctatgccttg tctgcaggag gaccgataat gcatcctcaa
    19381 ctcaatgcca tcgtcctggt acccatgttt tcacacagcc taagttcccg accactcgtt
    19441 atcgatgggg aagcagaaat tgaattgtat atcagcaaat caaatgaaac cgatttgcgt
    19501 atcagttgtg atggtcacga atcccgtgtc gttaaaccgg gacagaaagt agcagtcaaa
    19561 aaaaacggta atcgattgcg tttgttacac cctcttgatt atcattatta tgatacatta
    19621 cgaagcaaac ttggttggga atcaaagcat cagggataaa acatgctcac gacattgcgc
    19681 attgaacatt ttgctattgt taaacaactg gaactcgatt ttacacaagg catgaccacc
    19741 tttacgggcg aaacaggcgc tggcaaatcc atcatgattg acgccttgct gttagcatta
    19801 ggtgaaaaag cagatccttc tgtagtccgc cccggcgaag aaaaatgtga tatcacagcc
    19861 ggattttgct tcgatgaatc atcagaaccc gctcaatggt tagcagaaca cgacatttct
    19921 tgccatgatg gtgaaatcta tctaaggcgt attatttacg cagaaggtcg ttccaaatct
    19981 tatattaatg gccaaccttt ccctttacaa aaaataaagg aattaagtga aaaactggtg
    20041 cacatccatg ggcaacacca gcatcaaaca ctcatgcaac atgcaacaca tagacaacaa
    20101 cttgatcgct atgccaataa ccacctcttg ttaaatgatg tcaatacact ctataaacaa
    20161 catcaacaaa tagtacaaga agtaaatgca ttacaaagtc aggagcaaca agcagaccgc
    20221 attaaattat tacaatttca aattgatgaa ttacaagcgc tggatatcca ggatggtgaa
    20281 ctggaagcct tacatcaaga gcatcaaatt ttgcatcatg ccagagagta tctgcagtta
    20341 agtcaacaga tcaatgagct attgaatagt gatgatcaac cgaatatttg ctctggttta
    20401 aaccagattt ttcaactcat ccagcaactc cctcaagaac aagcgcaaat aaaaaacaca
    20461 gctgagttaa ttaattcagc cttgattcaa tgtgaagaag caatcaatga aatgcagcaa
    20521 ttcgcagata aaattcagct ggatccggaa agattgcaag aggtggaagc ccgaatcagc
    20581 tctattcatc aactagcccg gaagtaccat cttgatagca aatcgcttca ggagcatgaa
    20641 aaaacattac aaaatgaatt ggaaagtttg caaaatgtag aaagcaaatt ggcacatctg
    20701 catttgcaat tggctcaaat caataaggca tatgaaagcg ccgcattaaa attaagagaa
    20761 tcacgcaaaa ttcacgcaaa aaaactggct caagaaataa cggccagcat tcagcagtta
    20821 gggatgccaa aaggatggat tgaaattgaa atcactcctt tagaaaaaat gcaagctcac
    20881 ggattggata aagtggaata caaggtatgt actaaccctg ggatggaacc tgattcattg
    20941 aacaaaattg cctctggcgg agaattatcc cgaataagtc ttgcaataca gatgattaca
    21001 gcacaaaaag gttcaactcc taccttatta tttgatgagg tcgatgtggg tattggtggc
    21061 tcgactgctg cactagttgg aaaaatgctg agaaaacttg gagaaagatt gcaggttttt
    21121 tgtgttaccc accagccgca agttgctgcc tcggcacacc atcacttttt agtcaccaaa
    21181 catagtaata acaatcaaac ttttactcat atttcattgc ttaaagcatc cgataaaatt
    21241 gccgagatcg ctcgcatgat gggtgggctc actataacag aacaaacact ttctcatgcg
    21301 aaagagctga tatcgctgag caatgagtga acacttcgat cattttcggg tgaaatttta
    21361 ccaatctatt gatttaattt cctatttttt atacaaaatc taaattaatt caaaaaatta
    21421 aaaaaaaatt aataaaaaaa cagcacaatt aataaaataa tgctacaatg gatgcgtaac
    21481 aatttaggaa attaacatgt cagaaaaaat tcgtggtaca gtcaagtggt ttaatgagtc
    21541 caaaggtttc ggttttttag aaagtggcgg caaagattat tttgtgcatt ttagtgcgat
    21601 ccaaggcagt ggttttaaaa ccctggctga aggcgctact gtaatgttta aagcttgcaa
    21661 tggtcaaaaa ggacctcaag ctgaggaagt tgaagtagtc taattttttt atattagccc
    21721 tgttttttta agtataattt aatccttaga aaagcagggc tttttttatc tacaacattc
    21781 ttaaaatttc tgttctgcac ttaaaaaaac tctgctcaag caattgcttt ggcaaatatt
    21841 ggttaaagcc agctcaatta tatgataaag tttgtgccat cattcaattc aatagcgctc
    21901 aaattattat tggcaaatac ctgtgctgtt aatagctgtt actagcattc tgtttttcaa
    21961 tggaggcagc atagtagata aatatcaatc ccccatcgtt tctggtagac tgcataaaga
    22021 cgttcggtca tatcttgtct attataaatt gtttctgtac aaactaatat ggttctcatc
    22081 aaactcttgt ccaacttctt cttgaaccaa ttctgttgtt attcttacct tattagagtt
    22141 atccatgtca gtttgattgg tgatggtttt ctcatgacta tcatcttttt ctgcccacaa
    22201 ctttgctgga ttttctcgta atatttcaga aggtgactta actttcatat catgaaacac
    22261 agtagaatat gagttacttt tgctatcaat gagatctatt ttcgaatgct cagattttga
    22321 ttgtttttgt tccttttttt tcagtcttga aacggaatca tatgtaaaca tacttgatag
    22381 gatgtctctt aaaaatttac caattccttc tcttatgaat tctcctatgg cacttaaata
    22441 tgaaccaatt tttgagagta tactctcaaa aaagtaagat tctgtttgtc tttttgatga
    22501 agaattgttc ccttgttttt ccctctcttg ctgaagttct ttgcttgttt ttttaatttg
    22561 agagggttga agcaatacat cttgattttt cggattgatt tgttttagct caaccattag
    22621 gtttttcttt ttattgtaag catcacctgc tttgtcaagg tatctaaata tattaagatt
    22681 catctcttgt tcagcttctg ttcttagata aaagaaatga gcctcgggca gtcctttagc
    22741 accaacttcg ggatggaatt gcgtggttat aataggaaca ccaaactctg attctgctac
    22801 ttcattggat tcattatcac ttgcagtacc tgtatgttga aagcctgctg ctaatttgga
    22861 aacaacttgc tcatgcgcgc caaaaaactc cgtttcataa tgcggggaag taagagaatg
    22921 agaatcttct aatccattta aactcgtttt tatattttgt agttctgttt ttaattgatt
    22981 agtctcgcct atttctccta ctaattctct taaatcaaat aactcttctt ctatatttaa
    23041 tccggtcaac tttaataaaa gctgcacttc agatttatct aattcttcta attttcctaa
    23101 tgcatctaat ttccctgctt cctgtactag gtttaatttt tccaatgcat ttattttttc
    23161 tgataatcta ttatattggt tcattctttc attaaacgct tttctggctg tatccatttc
    23221 tttctggact tgtttgccac caataatttg agctattaat gaacccttat gcactttgat
    23281 tgcatcataa ttcatgactt tttgtgtatt taatttgtca gaatttaaat cgcatatttc
    23341 tccaccgcca tatactgcaa tgacttgatg cccaccacaa acacctagaa taggcatacc
    23401 tttttctgtc gctacatgca ctaatgccaa ttcagcaatt gttctggaaa aatcataatt
    23461 ttgatcggga tctcttgttt gattaaaaag ctcaggatca atcattgaat tattcccaga
    23521 taatgctaac acatcaacat ttttaagtaa ttccattgct tttgctttgg cgtgtgcaaa
    23581 taatctttgt cggccttctt tagtggaata caacttatca aattcttctt tgggtatgtc
    23641 ttccatcatt ttcctgtagt cgccgtcaat aacttcggaa ccgacatgtc taaatgattc
    23701 tttcaacgaa agaacactgc ttccaccaac ttcaggacta taggtaacaa ttactcttgc
    23761 catgatcata agcacatttg aatgagcaaa tgatattata aatgatcatt attaagggat
    23821 ccttatttat tggtttggtt ttgatcaatt ttcaatttca ggtaattatt tgatgaaact
    23881 gaaaaattaa aaggagttct ggacaagagc aaattatttg ccgccgtgaa ctatgacctt
    23941 caccttgata ccataggata caatatagag cctgattaat ggccttattc ctgaaacact
    24001 atttacccta tttgttttgc ctgcgattta caacatcatc aaatccaaac taattgattt
    24061 acaaaaacaa gcagcaaaaa tcggtgcagc aaataataaa gatttattta aaatgcaata
    24121 agtggatatg gatttttgca aatactatca gtattctagg caacatgatt tccccgctga
    24181 cttatttatg acttgtaaag gatctacggt attaatggtt ccaccggaag gattttgctt
    24241 caattatgaa acatcaacca gcaactcttt ccaatcattc ctttctatta aggatatagg
    24301 taacaaggca ttagcagaat ttactgcgat ggtgttacaa ctggaaaaag aagagataaa
    24361 agtattaacc ctctcacaaa gccgggattt acccgatgcc gtctttccca ataactggtt
    24421 ttcaacacat attgatgcaa gagggaataa tattcttatt atttatccat tattagctaa
    24481 aaacagacaa gcagaagtca atatcaaagg attaatggaa gtactgaaca gagctcaatt
    24541 tgaagttcat gaaataattg acttaagaaa cgataaggat gaaatccttg aaggaactgg
    24601 cagcctggtt ttagatagag aaaaccgcct tctttatgcg tctttatccc cgagaacctc
    24661 cctgaatttg gtaaacaaaa ttgccgatat tctgggatac acccctattg tatttaatag
    24721 tgtagatagg cagaacaaac ccgtttatca taccaatgtc ataatgggtc taagcaaatg
    24781 ttacgctatt atttgcctgg actctattaa tgatgctcaa caaaaagcaa tactttcaga
    24841 aagtttcaaa gagacaggca aaacaatgat tgacattagt tggaatcaag cacagcacat
    24901 gtgtggtaat gttttggaac tttataacaa aaaaggtgag agccttttaa ttttatccga
    24961 gcaagctaaa agacacttta ctcatgagca gctaagaatc atccaacaac actccagatt
    25021 aatatcaaca gatatcccta caatagaagc cattggaggt ggaagcgcac gatgcatgat
    25081 ggctgagatt ttttattaaa agttcaaaaa attttgctac cagtttaatt tcctttcaga
    25141 tattctctaa aacgagactt aagaaaaacc ccaaggtcaa ggtaaaaaaa gattggaggt
    25201 ttacttaagg cctgtaaaaa actaatcatt caggcaggga atcatcatga aaattagtga
    25261 attaaaaaaa ctggttgctg aattaaataa agaggtttca ccaaaggtta catgcacaaa
    25321 cataattaac ctggctggta atttatcaga aatcattgag tattttgagc aggatgaaca
    25381 tgttggtcca gagcttatat accgaataga agcggtgatt tgtgaattct ggaagctagt
    25441 tagtctcaca ttaccttatg aagaatggca aagcagtatt caagttgctc catggcttac
    25501 attacagcaa tcattatcca aagccgggtt actgcctacg gatttccacc atcctatttt
    25561 gtaccagcgc ttaaaagaac gctatgaaag ctttggtcac agcgagttag gaattgacca
    25621 gctcttgccc ttgcttatcc gttgcagccg catgactggg tatgccaata aagatccaca
    25681 atctcttgat acttatcctc actcaccatt aaacaaacaa atcgaagcga gaagaccaca
    25741 ggaacttgcc aagctaaaag acatcctctg ccttttaaga gccatcttct atttaattca
    25801 tcattgttgc accattgagc aattgacgtt aattccttac ctgatttatt ttcgtagtcc
    25861 cactacagat gaagaaagac gatctgaatt ggccattttt aactggctaa cccaaaaacc
    25921 agctgattgc cttgaattct ttaaaaccaa tgaagattac attgacacaa gatcatttag
    25981 gcaaattagt gaattggcac cattacgccc ttttatccca actgcgcgta gtgattttat
    26041 taaaataact aatagagaac actggatata cccttttatt caaagcagga caaatacatc
    26101 aagatcagag tatgatttgc tgaatgatgc agtcaattgg ttagacactg attttgcaac
    26161 tgagaaagat aaatcctatc atgctgcttt ggagtttgcc cataccgtca aaaagcaagc
    26221 aaacatttta acccaaaggg aaatgaaaat agtgcattcc gcgctctatg tcttttgtct
    26281 ggacaaatac gtaaaacatc gtaaagcaga tcctcgtcca aggtgcactc cgttttcact
    26341 ttcgggagaa acaaaatgcc aagcagccga aaaaaaacag caggagatac tgggaaaacc
    26401 aacaaagttt ggatttttcg aaaatctggc cttgaatgaa ggaagattaa aaacactgac
    26461 caagactttt g
//
LOCUS       DBNCWK010000028        26307 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.028, whole genome shotgun sequence.
ACCESSION   DBNCWK010000028 DBNCWK010000000
VERSION     DBNCWK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26307
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.028"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            67..1836
                     /locus_tag="AC06IO_001090"
     CDS             67..1836
                     /locus_tag="AC06IO_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIO9874293.1"
                     /translation="MNQEISNFSTFNFSSALNKALEDMKFITPSPIQAQTIPLILQGR
                     DAIALAQTGTGKTAAFALPILQNLSPEISTTQALILAPTRELAIQVAEQFELLSKYQR
                     NVTIAVLCGGQEYGRQLKQLRSGAQVVVGTPGRILDHIDKGTLLLNNLKTFILDEADE
                     MLRMGFIEDVETILEKLPEKKQMALFSATMPYRIRQIANTYLNDPASIEIRMETATVK
                     SIEQRFLFASVHQKPDALIRVLEVEDYQGVIVFVRTKSSTEEVAELLQQHGLRAMAIH
                     GDITQSLRERIIAQFKQGAIDILVATDVAARGLDVERVTHVINYDMPHDNETYVHRIG
                     RTGRAGRSGVTILFVTPKESRLISSIERHTRQRIEKVQVPNDHMIQMARQQRFMSNIT
                     TRLNHEHVYSYKRIVEEYIKDHDVSAVDVAAALALLLHKDMPWRKELNMPKAASAFKE
                     ERARAGEKGLDLRGRFKEDRKSSFADRKGARGSKKDFAKDFSNERKKFNDGRKAVNQE
                     SVAQDLFRIDVGKIHGVKPGNIVGAIANEAGLQSRYITGLKIHEDHSTVRLPKGMPKE
                     VMKDLTKAWVCGRQLNLTLIGSA"
     gene            2185..2403
                     /locus_tag="AC06IO_001091"
     CDS             2185..2403
                     /locus_tag="AC06IO_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874294.1"
                     /translation="MKKKHEPGFFGRETKNIKDIESNRYDPDCPIRRADKSRGFSQKA
                     SGQFQLRIKEEVKEALDDIRNESAYKFN"
     gene            complement(2562..3077)
                     /gene="mavE"
                     /locus_tag="AC06IO_001092"
     CDS             complement(2562..3077)
                     /gene="mavE"
                     /locus_tag="AC06IO_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavE"
                     /protein_id="HIO9874295.1"
                     /translation="MTRFERNFLINSLMFLETILSVDKKLDDAIHHFTQGQYENPRYQ
                     INSRITNADDWSKEDKLKFTSAIAEAIALVSEKYENPTSETTEQIQSARNILLNDYVP
                     LLTANTDPENRLKSVRENSSQIRKELIAKLKDEVPYKSQFENPYVLFPFVAATVAVAA
                     TAASVLFGNKP"
     gene            3347..4276
                     /gene="plaA"
                     /locus_tag="AC06IO_001093"
     CDS             3347..4276
                     /gene="plaA"
                     /locus_tag="AC06IO_001093"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family lysophospholipase PlaA"
                     /protein_id="HIO9874296.1"
                     /translation="MKLLASLCAFLLSGVVSATPLNNIVVFGDSLSDNGNLYEYMKHQ
                     LPQSPPYFEGRFSNGPVWIERLAASYFPNDPNSHLLDYAFGGAGVSVDEEDDEVFFTL
                     RREVNSYLLAHQDKASPDSLFVIWIGANNYLGMPVEVEETLKNVNRGITDSIQRLVDK
                     GAKHILVLNLPDLGRTPAALEFGSVEEMTYFSTQHNNALSNTVDYFKKTYPEVEWLFF
                     DTGSHFDHVIEHAAEYGFTNITGTCSFSIVDEITKNSVLKMVASVKPELTENACDGYL
                     FFDLVHPTALAHKIMAEKARLMLDEAGVEFAEN"
     gene            4469..5242
                     /locus_tag="AC06IO_001094"
     CDS             4469..5242
                     /locus_tag="AC06IO_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HIO9874297.1"
                     /translation="MNQIKWIVLLFCFVVSNVFADKLDITVDQKKIILPYWLSRPVSY
                     GAVIIVSGGETAQWSLLLEQFAKGLSRNGWSVVLLNCTKNNSIPWINQLPEVISTLRQ
                     NNNKRIVLVHYGDQLNLALEYFSKPQSKMINGLVMLSAYDLNRNLQKVPRLRFPLFDI
                     VGQFDYDMVRQQRKSREEKFKENSYLAIDIPGASHDYQYSQQLLLAFVHGWMAKLPEF
                     EPQPPPILVSYLEPVYSKASSIVSTNKLDYQPLVSQINS"
     gene            complement(5209..6099)
                     /gene="djlA"
                     /locus_tag="AC06IO_001095"
     CDS             complement(5209..6099)
                     /gene="djlA"
                     /locus_tag="AC06IO_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HIO9874298.1"
                     /translation="MNLRDFFIITTWWGKILGAFFGYLTAGPVGALFGILVGNFFDRG
                     LVSYYSNPHWLYHAEKQRIVQKAFFEATFSIMGHVAKSDGRVSEQEISMAKSIMNEMK
                     LSKGQKDLAKRLFNEGKQPDFNVSLALIQLQRICKDNRDLLKLFVDIQYRAAQVDGLS
                     SQKIHALDNIFTHLGFAPLHKQYRFYEDFGSYFQQEQSKQHYHNQQESKHTSSSQGQQ
                     GYKPQSPPNTLAHAFALLEVSPNANKQEVRRAYRRLLSRNHPDKLIAQGLPEEMIKLA
                     NDKTHQIMKAYELICETKGW"
     gene            6254..7513
                     /gene="waaA"
                     /locus_tag="AC06IO_001096"
     CDS             6254..7513
                     /gene="waaA"
                     /locus_tag="AC06IO_001096"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HIO9874299.1"
                     /translation="MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQE
                     NAPVDVWLHAVSLGEVIAATPLIDAMLNKHWSVLVTTMTPTGSERVKSRFGHKVAHQY
                     LPYDLPWVLKRFFKRTKPRVGIIMETELWPNLINQAHASGVSLFLANGRLSDRSLQGY
                     LKLKFLFKPVLNQFSGVLTQSNEDAERFIALGANADLVHVLGNMKFDLQIDSVDSSQY
                     RELKNHWGEDRLTVIAASTHDDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQ
                     LSVQAGFNTGCRSNLDTVSRENEVVILDSLGELLGFYQISDFAFVGGSLVPVGGHNVL
                     EPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIKLYQDKESQNTMV
                     ANASSVLESNKGSVVRYLQKIESVLGY"
     gene            complement(7518..8351)
                     /locus_tag="AC06IO_001097"
     CDS             complement(7518..8351)
                     /locus_tag="AC06IO_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9874300.1"
                     /translation="MFFMYFTNYGYRLNQIKKGSDQHWLFLPGGPGLGSEYLASFCDK
                     LTLQGTISLVDFPKDGNNSEGQLDFVSWQEGLISLLKSLNKPILVTHSFSGMFTLNFP
                     EIENHLSGLVLMNTTTTNSFFQHVNEMRQKYNLPDLVPAASQYHLYPSNETYKKFWDT
                     YKHYCFTREELPIGEQMMELFAYNNESYHYAIEHFYNDFHYKWHPQTIPAMTIASELD
                     YICPPAIFIQDKNFQCQNVINKVIYKAGHCPWILHLEQVQKCFDEFSGMIQEINMERI
                     L"
     gene            complement(8421..8852)
                     /gene="dksA"
                     /locus_tag="AC06IO_001098"
     CDS             complement(8421..8852)
                     /gene="dksA"
                     /locus_tag="AC06IO_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIO9874301.1"
                     /translation="MKNDAIGNMGIAPYQETEGEEYMNEKQLAHIEKILLAWRQSLME
                     EVDRTVSHMKDEAANFPDPSDRASQEEEFSIELRTRDRERKLIKKIEDALERLRNEDF
                     GYCEACGIEIGLKRLEARPTATLCIDCKTLSEIKERQNQGA"
     gene            complement(8996..9859)
                     /gene="prmC"
                     /locus_tag="AC06IO_001099"
     CDS             complement(8996..9859)
                     /gene="prmC"
                     /locus_tag="AC06IO_001099"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIO9874302.1"
                     /translation="MIDIRSLLEQALQQFKVKNQETKLEAELLLCHVLNKNRAYLFAH
                     PDALVSPEQIETYLQMIKQRAEGLPIAYITGQREFWSLSLKVTPNVLIPRHETERLVE
                     LALELIPDKENVSVLDLGTGSGAIALALAKERPLWHIDACDFSKEALELARYNAKTLG
                     LNNINFCHSYWFNNLPLKQYHAIVSNPPYIAENDPHLKQGDVRFEPTSALVSSQDGLA
                     DLQYIIQHSYEYLLPDGLLLVEHGFEQKNEISTILNQLGYKNIHCWQDLQGHDRVSGG
                     QRPSNRNNKPL"
     gene            complement(9852..10940)
                     /gene="prfA"
                     /locus_tag="AC06IO_001100"
     CDS             complement(9852..10940)
                     /gene="prfA"
                     /locus_tag="AC06IO_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIO9874303.1"
                     /translation="MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQ
                     LEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMAEEEIDTVKKQIEELDEQLQWHL
                     IPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
                     YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQ
                     INPDDLRIDTYRSSGAGGQHVNKTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLL
                     KTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDI
                     MEGNLSLVIDPLKREYHAELLADLGRHD"
     gene            complement(10924..12198)
                     /gene="hemA"
                     /locus_tag="AC06IO_001101"
     CDS             complement(10924..12198)
                     /gene="hemA"
                     /locus_tag="AC06IO_001101"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIO9874304.1"
                     /translation="MVFVACGLNHKTAPIHVREKVALQPAMQDSLLSSLLDLPEVNEA
                     AILSTCNRTEIYCDTNTPEVLGNWLAHEHQLSEELLSQFLYIHQGKEGIKHTLRVASG
                     LDSMMIGEPQILGQMKQAYQHACRLGTVKTQLRPVFEYIFRASKRIRTRSGIGANPVS
                     IAYAAVQLIGQLFKNYHSLSVFLIGSGETASLVAKYLHQHGVHRFLIASRTLENAQKL
                     AETFGGKTLSIGDIPQYLPLADVVISATACPLPFINKSLVEHALEQRNHAPMFLLDLA
                     VPRDIEGNVNELEQVHLYNVDDLQSMIEKGMDERRNAALQAEQLIESELDNYIRWHRS
                     LRAKDVICDYRNQMHTLAQQELQRALKKISAGQNQQDVLNEFSMRLVNKLTHNPTIGL
                     RQMAWDNREDLLDLARYLFDTTANQSLYEEIS"
     gene            complement(12322..12477)
                     /locus_tag="AC06IO_001102"
     CDS             complement(12322..12477)
                     /locus_tag="AC06IO_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874305.1"
                     /translation="MAIHVGGKTQQDFRKSLFSLLDMSPFEDRESKLNPLPANFPKWD
                     NLEEEGG"
     gene            12516..12794
                     /locus_tag="AC06IO_001103"
     CDS             12516..12794
                     /locus_tag="AC06IO_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874306.1"
                     /translation="MDIILQGQHNAIETVESLERVLKLFKERYQVDGFREIHLTVTLV
                     DEQGDEVELVDSDTNQPYRIFEVYRKGYELAGRKGIPMLQLVVDNTHK"
     gene            12851..13924
                     /locus_tag="AC06IO_001104"
     CDS             12851..13924
                     /locus_tag="AC06IO_001104"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIO9874307.1"
                     /translation="MIMALVAIILTLILVIGIHELGHALAARLFQVKISKISIGFGKP
                     LIQWQTQSGCNWIWAMWPLGGYVQLLNSRISPVKPQENAYCFDKKPIWIRVLILLSGA
                     IANLVTAWIALVLVFYIGISYKQPQIQSVKLDSLAAKAGIQAGDQWVSVESYPTDSWQ
                     GVGMQLVIHWGQKDVRIVLRQGNQQLKQLNLDLSQIEFTSKDGSLLESLGIKPDLSAV
                     SSLTRYPSLLASMQKAFAEIIHTMYFFIMILKQLFLGVIPFSILLGPLAIFSVSVASL
                     TQGVIVFLLFIATLSLAVALVNLFPIPGLDGGSILYSVIEKIRGKPVSVAVEVLLHRL
                     MIILFCVLLVHLLMNDLNRYLHH"
     gene            complement(13953..14657)
                     /locus_tag="AC06IO_001105"
     CDS             complement(13953..14657)
                     /locus_tag="AC06IO_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIO9874308.1"
                     /translation="MLQKFLSITQNLRLPSICTLCNQFHKSQLAVCSNCMEFMKQIGS
                     CCQYCAYPLSDDTYLVCGQCVRKRPSFDRAYIAYRFEEPLRSLIHQFKYHNGLYLASF
                     LKQLLLNALPKSALKPDCLIPVPMHPKRLKRRGFNQAAVLTGLLARQLNIPYDLYYCQ
                     KIINTASQANLDGEQRRKNLRHAFYVPPVTYEHVMIVDDLLTTGSTANEMAHTLKNAG
                     VKRVDICCCARAVTKN"
     gene            14701..15555
                     /gene="bioC"
                     /locus_tag="AC06IO_001106"
     CDS             14701..15555
                     /gene="bioC"
                     /locus_tag="AC06IO_001106"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HIO9874309.1"
                     /translation="MTVNNEISKAFSKHADDYERVAKVQKEIGSRLFERLQYLKIAPR
                     RILDLGCGPGFFSKELALLYPKAQIVGMDLSFAMLEQARKKQGWRRKWPLVSADMQKM
                     PFATGVFDLVFANQVIHWSSSLGTVFRELNRVMNVNGCLMFTTLGPDTFKELQTAWSA
                     ANQYAHVNEFVDMHDIGDCLIAEHFMDPVIDMELLSIHYETLPQLLLALKTQGVRNIN
                     PKRNHGLTGKSAWKQFEEQYATMRTTTGKYPLTYEVVYGQAWKGAQRKMEQGIETWIP
                     VSRVVKKS"
     gene            complement(15599..16495)
                     /locus_tag="AC06IO_001107"
     CDS             complement(15599..16495)
                     /locus_tag="AC06IO_001107"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIO9874310.1"
                     /translation="MNKQVIDKYTQRHELYLEQLLNEIVIPAPQIRSALHYALFSGGK
                     RIRPILVYLAGDLIDVDQEVLDIIAAALELTHCYSLIHDDLPAMDNDDLRRGKPSCHK
                     AFDEATAILVGDGMQALAIEVVLMRLSPLLPAAQVVAITQVLVNASGISGMVSGQSLD
                     LSELAKSSVTEEQLRGIHLLKTGKLILACFEMVLAAQHEVSEQIKSALRTYGKHIGLV
                     FQMQDDYLDLYAPTQILGKGRSSDQANQKTTFATLFNKQQLEEEIAVHYQTAMDSLRL
                     FGSKAAALIELTKQLQNRSNLS"
     gene            complement(16476..16706)
                     /locus_tag="AC06IO_001108"
     CDS             complement(16476..16706)
                     /locus_tag="AC06IO_001108"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIO9874311.1"
                     /translation="MSKGIHFEQSITELEEIVRQLEKGELSLEESLKQFEKGISLARR
                     CQNALNQAEQKIETLTGTDSNIELDSDEQTSD"
     gene            complement(16900..17283)
                     /gene="lem22"
                     /locus_tag="AC06IO_001109"
     CDS             complement(16900..17283)
                     /gene="lem22"
                     /locus_tag="AC06IO_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem22"
                     /protein_id="HIO9874312.1"
                     /translation="MSAFKKGQNPVTELTRLKEYMEDQIAKAKESSSLTAQLKFLENA
                     HTEHFVKMGSLTTIYKGGNEVVDRLKIEIRSLYEEMLALKDKCRDQIQQHETSIKNSP
                     TFFTTKNNKTKDEEFDLDFDKAFGL"
     gene            complement(17547..18425)
                     /locus_tag="AC06IO_001110"
     CDS             complement(17547..18425)
                     /locus_tag="AC06IO_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2327 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874313.1"
                     /translation="MAKFTVGQDPVKGLTELKAYMEEQISKIKKATSEQDIDQLLIDT
                     LNEYDDKMGSLSKVYKGGNEQVEQLKIDVRKLYEPLRDQFSHKHPLTLVSEVQTKLEE
                     QRIQAEVEKLKLEEQLKLEKQLEEEKLKLAQQSEEEKPKLENQQEETITQALQKVDGI
                     LQELTLKIDRVDQHQYKKAHDTANTLLQSLIAARDEYERDLRANEFSPELAGRKFKLA
                     CQDAVKIAKPVLEKDLGWGDYLKNLLKCLGNAVITVFTFGYQQGFFAYARPDSAKAVE
                     KAEEDLGLRQAASSPK"
     gene            18659..20011
                     /gene="radA"
                     /locus_tag="AC06IO_001111"
     CDS             18659..20011
                     /gene="radA"
                     /locus_tag="AC06IO_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIO9874314.1"
                     /translation="MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIMPESKTL
                     RSGQWANQRSVITAVEDVVMDKIVRMDCGISELNRVLGGGLVYGSVVLIGGDPGIGKS
                     TLLLQTLANLSMQETVLYVTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQ
                     KEKPRIIVVDSVQTIFTEAISSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEG
                     TLAGPRVLEHMVDSVLYFEGQNDSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANP
                     SAIFLSRQPDPTPGSAVMVTWEGSRPMLVEVQALVDEAHGQQAKRVTAGLEHNRLAIL
                     LAVLHRHGGIATYDQDVFINVVGGVKVTETASDLALLAAVISSLRNRIFDNETIIFGE
                     VGLAGEIRPVQSGQERIKEAIKHGFKRAIVPFANAPKQQIDSMTVEPVKHLRDVLEKM
                     "
     gene            20008..20844
                     /locus_tag="AC06IO_001112"
     CDS             20008..20844
                     /locus_tag="AC06IO_001112"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIO9874315.1"
                     /translation="MSYQKSITLEYKMNEDTIRDFNRNGAVCLRQFLNAEEIAILKEG
                     IEFNLSSLSPRAKIASRPDDPGHFVEDFCTWQNNPFYQRIIFGTNLGVAAAQIMQSQT
                     VRLYHDHLLVKEPGTRQVTPWHQDQPYYNIEGKQNCSFWIPVDPVSRSSTLEFVAGSH
                     LGPWLMPRTFLDNQAKWFPEGTLQELPPIEEARAKYPIIGWEIEPGDVVCFHMLTLHM
                     ARGVEGRHRRRVFSVRFLGDDIKHAPRAWPTSPDFPGLTAAIPAGASMDHPLFPCLPL
                     KI"
     gene            21132..23060
                     /locus_tag="AC06IO_001113"
     CDS             21132..23060
                     /locus_tag="AC06IO_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-gulono-gamma-lactone oxidase"
                     /protein_id="HIO9874316.1"
                     /translation="MTLPTELKSKFIQILNQHKISHSVGWSNYMESVNNPVAIVVEVK
                     NEKEVQTVMMAIKELNSDREPKDKVTVRATAGWTDKPGKSCCFFPWSEAQESQYNEGF
                     SFSQVVGGKASPDKAGTDVIIRFAKKFHHAKVIGPLKNPPVFNPDNPIHRLPVTLVEV
                     SAGMQVADFADFLREHQLSLSTVSMISWVTPVGLAGTAGHGTGRDEPAFSGLIESVTV
                     CDMDGNLREINSTHPDFKDLIGGHSGLLGIVVSMKLRAVKAFNLCETIELFPNTREMT
                     GRLSDILKNNQYISIMGMPSYGCPESDKLVSKWQIRQWNYTTDKPGKKEKAPYDPDVR
                     SFAQELQVRIGASVMDFLLDSGLKHLLPSFMLLSAAVVTGTRGTSPKVDYENHITHPQ
                     VAFPKEMRDVSYLIPVKDNEAGQKLELILQKMEAMLNEAGSKGEFPVTYAIYVRYLKG
                     TNGGLSTSFTSSDDERILAIDVVTHPEAPGITNFEKGFMSFLKEMNIIPRNHLGKNFP
                     AGVVHYDQFLGAEKVSKYKQALERFYCSPGKNDGAERLSMSPFYTPYLQSMLSASAPT
                     NQLVDENKYEQRIERLHEEHSDLECAEFLSKLYAEVSRMSTFSEEALTAKKGFLEACK
                     AELEHRNSRLEAPVPLLS"
     gene            23150..24271
                     /locus_tag="AC06IO_001114"
     CDS             23150..24271
                     /locus_tag="AC06IO_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIO9874317.1"
                     /translation="MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKS
                     NQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGRFRINLFRQRGEVAMVVRYIKGE
                     IPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
                     PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYA
                     ETGHLCISTLHANNANQALDRVINFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQR
                     VAAVEILINSPYISDLIEKGKIDDIKEAMARSTEQGMQTFDQALFNLYKEGKITKENA
                     IKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDKV"
     gene            24358..26283
                     /gene="ankK"
                     /locus_tag="AC06IO_001115"
     CDS             24358..26283
                     /gene="ankK"
                     /locus_tag="AC06IO_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkK/LegA5"
                     /protein_id="HIO9874318.1"
                     /translation="MPRVYNLKDIYLGAPSFSGHEVYLDAVYYPSDPSEKNFRVIYKK
                     NKFGNANLSRMEVAFSQLARLFLDNGLTSFQKMVVNDANKVQGLIVEHLNYVIENKEG
                     LKQPFYTLNAPRNGCDFTEKRVTSPNEIPFYFLDKLPQGFFNQLLAAEKNNKLSIDYA
                     SLASILATSYTLEEDDLHKGNFGFYLVKKQGKPRVVFFKIDHDLMFVDSIMSFTTRRF
                     CHLFDGCDAFDITEEDLLKFPNLKYSANGYWPTKTSIFYKPWDNKDYRTYAEIQAFAD
                     LSHVEEFNKAKWRSFYKHILISQSQMEATLKACFDENNSSDRAHISLVIQAMLARQAR
                     LKAMLFSLKDFRDFILSQNGKERDLLCHEILNNLPEEERKSFENEIRQSLDYNHNLCC
                     SGLFEDGDTPLHIAIKSGDYRYDETIGMYGQFINTKNSSGKTPLDIALQMAGQSKVHP
                     ADVRKDYRFIMKHLLANGANQTKQFEEFDKIENIRSYQFHTPYLNKAIKAKTYHELKE
                     VLRDIGEDHQYCLKFKKMLAVECISEFIKANQDNLSLRGILLKLKKEVDGKGTKSENA
                     ALMYIRQLRSRLWIVRQIRGLYGWSTTQGEIDYMIDKELARLDTKDLKRLSLFDSRDS
                     STLDNVFLDISLSKNKI"
BASE COUNT         7710 a         4678 c         5589 g         8330 t
ORIGIN      
        1 tataatgcac attttttata ttagttagcc ttttcagaaa gacgctttat tatattcagg
       61 aacagtatga atcaagaaat atcaaacttc tctactttta atttttctag tgctttaaat
      121 aaggcgttag aagatatgaa gtttattacg ccatcgccta tccaggctca aaccattcct
      181 ttgatattgc aaggtcgtga tgccattgct cttgcgcaaa ctggtacagg taaaacagcg
      241 gcttttgcat taccaatttt gcaaaatttg tcccctgaga tatctaccac tcaggctttg
      301 attttagccc ctactcgtga attggcaatc caggttgcag agcaatttga attattaagc
      361 aagtaccaac gcaatgtgac gattgctgtt ctttgcggtg ggcaagaata tggtcgtcaa
      421 ttaaaacaat tgcgtagtgg tgcgcaagtg gttgttggaa cgccaggacg tattcttgat
      481 catatcgata aaggtacctt actcttaaat aatctgaaga catttattct tgatgaagca
      541 gatgaaatgt tgcgtatggg tttcattgaa gatgtagaaa ccattttaga aaaactgcct
      601 gaaaaaaagc aaatggcatt gttttcagca accatgccct atcgaattcg tcaaatagcc
      661 aatacttatt tgaatgaccc tgcctcaatt gaaatccgta tggaaactgc gacggtcaag
      721 tctattgagc aacggttttt atttgcttcc gtacatcaaa aaccggatgc gttaatccgg
      781 gtcttggaag tggaggatta tcaaggggtt atcgtttttg tacgcactaa aagcagtact
      841 gaggaagtag cagaactatt gcaacaacat ggattaagag caatggcaat tcatggtgat
      901 attactcagt ctttgcgtga acggattatt gcccaattta aacaaggagc gattgatatc
      961 cttgtagcga cggatgtcgc tgcaagagga ttggatgtcg agcgagttac tcatgtgatt
     1021 aattatgata tgccccatga caatgaaacc tatgttcaca gaattggtag aactgggagg
     1081 gctggccgtt ctggtgttac gattttattt gtaactccca aggagtcgcg tttaatttcc
     1141 agcattgaac gtcataccag gcaacgaatt gagaaagttc aagttcctaa tgaccatatg
     1201 atacagatgg ctcgtcaaca aagattcatg tctaacatta ctactcgact gaatcatgag
     1261 catgtgtatt cctacaaaag gattgtggaa gaatacatta aagatcatga cgtatcagca
     1321 gtcgatgttg cggctgctct tgctttattg ctgcataaag acatgccttg gagaaaagaa
     1381 ttaaatatgc ctaaagcagc ttcagcattc aaagaagaga gagcccgggc aggagaaaaa
     1441 ggattggatt tacgtggtcg atttaaggaa gatagaaaaa gttcttttgc tgatcgaaaa
     1501 ggtgcgcgag gaagcaagaa agattttgcc aaagactttt ctaatgaaag gaaaaaattt
     1561 aatgatggca ggaaggccgt taatcaagaa tcagtagccc aagatttatt tcgtattgat
     1621 gtaggcaaaa ttcatggagt caaacctggc aacatagtag gggctattgc taatgaggct
     1681 ggcttgcaaa gccgatatat tactggtctt aaaattcatg aagatcactc tactgttcgt
     1741 ttaccaaaag gtatgccaaa agaagtgatg aaggacttaa ccaaggcttg ggtttgtggc
     1801 cgccaactca atttaacatt aattggtagt gcctgaatag ctcagcagta aagtgctgtt
     1861 agcgctatca attggccttt aattattttg taaaacgatt tcgcctgtca gggcgagatc
     1921 ataacattaa gtacaataga tagcgcaaac aatggttcag attttattat ccccgcacag
     1981 gcagtaagct atctcttctc tatagcaaag ggaggggcaa gatttgaaat agattcccac
     2041 tcgcgtagga atgatatgca tatgtattat ttaatcaaag tatgatttta ccctgaagac
     2101 ctaccttttt cctggcttgt ttttctaatt taatacctct aatatggatt ggtgttctga
     2161 aatttaacac tgaggggcca aattatgaaa aagaaacatg aacccggttt tttcggtaga
     2221 gaaaccaaga atatcaaaga cattgaaagc aatagatacg atcctgattg cccaattcgg
     2281 agagctgata agtctcgtgg attcagccaa aaagcaagcg gccagtttca gctacgaata
     2341 aaagaagaag taaaagaagc gttagacgac attcgtaatg aatctgccta taaatttaat
     2401 taaccattat tttatctctg agtgtaagag atcagctctt tcttgaaacg accgtatttg
     2461 gtccatcttg aacggaaata accattggag tactgatgat ctgtatattt ctgagtttaa
     2521 tgaagcctga caagaggctt cattagtata aaacactacc tttatggttt gttgccaaac
     2581 aacactgacg cagcagtagc tgctacggct acagtggcag caacgaatgg gaaaagaaca
     2641 taggggtttt caaactgact tttatatggt acttcgtctt tgagtttggc aattaattct
     2701 tttcttatct gactactatt ttctctcaca gattttaatc tattctcagg atcggtgttc
     2761 gctgtaagta atggaacata gtcattcaat aatatatttc gggcactttg tatttgctct
     2821 gtagtctcac tggttggatt ttcatacttc tccgaaacaa gggctatggc ttctgcgatt
     2881 gcagaagtga attttagctt atcctccttg gaccagtcat cagcatttgt tatacgtgaa
     2941 ttaatttgat agcgggggtt ttcatattgg ccttgagtaa aatggtgaat tgcatcatcc
     3001 agcttcttgt ccacagagag tatggtttca agaaacatca agctattaat caggaaattt
     3061 ctttcaaatc tagtcattat tatggttctc ataaagaaat aagggctgac gacatttctg
     3121 ctgaatcgat tgcaagatat tgattttctg taccctgtta caaaagaaag cattatgaat
     3181 cgagtcaggg aagtgtttac aggatttact ataattaggt ttctgtttta gatcaacaaa
     3241 aaatttatat acagtaatgt gttaaatgcc gtggaaaaat tgataaatca ttatataatt
     3301 gaatgaacct aaagatataa aatcaatcta ttacttaagg ggtttcatga aactattagc
     3361 atctttgtgt gcttttttat tgtcgggagt tgttagcgcg acaccactta ataacatagt
     3421 tgtatttggt gatagtttgt cggataacgg taacttatat gaatacatga aacaccaact
     3481 tcctcaatcc cccccgtatt ttgaaggtcg tttttccaat ggcccagtat ggatagaacg
     3541 tctggcagct tcatacttcc caaatgatcc caattctcat ttgctggatt atgcttttgg
     3601 cggggcagga gtctctgtcg atgaagaaga tgatgaagtg ttttttacct taagaagaga
     3661 agtgaattct tatttgttag cacatcagga taaagcaagc cctgacagtc tttttgttat
     3721 ttggataggg gcaaataatt acctcggaat gcctgttgaa gtggaagaaa cgcttaaaaa
     3781 tgttaaccga gggattacgg atagcataca acgtttggta gacaagggtg cgaagcatat
     3841 tttagttctc aatttacctg atttgggaag aactcctgcc gccctggagt ttggctctgt
     3901 tgaagagatg acctatttct caacacagca taataatgca ttatccaata cagtggatta
     3961 ttttaaaaaa acttatcctg aggttgagtg gttgtttttt gacacgggta gtcattttga
     4021 ccatgtgata gaacatgctg cagagtatgg atttacaaat ataacaggta cctgctcctt
     4081 ttcgatagta gatgagatta ctaaaaactc cgttttgaag atggttgcgt ctgtgaaacc
     4141 agaattgaca gaaaacgcat gtgatggtta tttgtttttt gatttggttc atccgacagc
     4201 gttggcacat aaaataatgg cagaaaaagc gcgtttgatg ctggatgagg ctggtgtaga
     4261 gttcgccgag aattaaaaat tatttttatg gcaatagcga atgcaagttg catcgtcatt
     4321 caaattaatc cgcaatgata agattgggga ttttagactg agtgaaacct aatgagagaa
     4381 tggtgtattt gaacttcgca aatattctct cattcaactg gcactaacta gtccgtcttg
     4441 tccatcatag gcaattttat agggaaatat gaatcaaatt aaatggatag tactactatt
     4501 ttgttttgtt gtttccaatg tatttgcgga taagcttgac attaccgtcg atcaaaaaaa
     4561 aattatattg ccttattggc tgtcaaggcc tgtttcctat ggggcggtta tcattgttag
     4621 tggtggagag acagcgcaat ggtccttatt attggaacag tttgccaagg gattgtctcg
     4681 aaatggttgg tcagtcgtat tattgaattg tactaaaaat aactcgatac cctggattaa
     4741 ccaattaccg gaagtcatca gtactttaag gcagaataat aataaaagaa tcgttttagt
     4801 ccattatggt gatcaactta atctggcatt agagtatttt agcaagcccc aatccaagat
     4861 gatcaatggg ctagtgatgt tgtctgccta tgatttaaat agaaatttgc aaaaagtacc
     4921 tcggttgcgt tttccactgt ttgatattgt ggggcagttt gattatgaca tggtaaggca
     4981 gcaacgtaag agcagggaag aaaaatttaa agagaatagt tatttagcca ttgatatacc
     5041 tggtgctagt catgactacc aatacagtca acaattatta ttggcgtttg tccatggttg
     5101 gatggcaaag cttccagaat ttgagccaca gccaccgccg atattggttt cttatctgga
     5161 accggtttat tctaaagcaa gttcaatagt atcaaccaac aaattggact accaaccctt
     5221 ggtttcacag atcaattcat aggctttcat aatttgatga gttttatcgt tagccaattt
     5281 aatcatttct tcgggtaaac cttgagcaat taatttatct ggatggttac ggctcaatag
     5341 acgtctgtac gctcttctaa cctcctgttt gtttgcattc ggacttacct caagtaatgc
     5401 aaaagcatga gctaaagtat tagggggtga ttgaggtttg taaccctgtt gaccttgaga
     5461 tgaggaggta tgcttggatt cctgttgatt atgatagtgt tgtttggatt gttcttgctg
     5521 gaaataagaa ccaaaatcct cataaaaacg atactgtttg tgtaggggag cgaatcccag
     5581 atgcgtaaaa atgttatcta gtgcgtgaat tttttggcta cttaatccat ctacttgagc
     5641 tgccctatac tgaatatcta caaaaagctt taacaaatca cggttatcct tgcagattct
     5701 ttgtaattgg ataagagcaa gactgacatt gaaatcaggt tgtttccctt cattaaataa
     5761 tcgttttgcc aaatcctttt gccccttgct caatttcatt tcattcatga tggatttagc
     5821 catgctaatc tcttgttcag aaacacggcc atccgatttc gcaacatgcc ccattatgga
     5881 gaaagtggct tcaaagaaag ctttttgaac gattctttgt ttttctgcat ggtacagcca
     5941 atggggatta gagtaataac tgacaaggcc ccgatcaaaa aagtttccaa cgagaatgcc
     6001 aaacaaagcg cctactggcc cagcagttaa gtagccaaag aaggcaccaa gtatttttcc
     6061 ccaccaggtt gttattataa agaaatcgcg taagttcatt atcagaaatt acctataaag
     6121 agatgtgtta aatttaattt caagttctaa taatatagat tgaggacaac atagtctcta
     6181 attgaattgt aacatatcgc ataaggaact gttttaggta tatactttac ctttttgtgt
     6241 ttggtgcctg tttatgcgtt ttgtgtattc ctttttaatg tacttactga ctccttactt
     6301 gctttttcgc ctgtggcgga aaggtagaaa gttgccagca tacaggcaaa ggataggaga
     6361 gcgtttttgt ctgggaatac aagaaaatgc accagttgat gtatggcttc atgcggtttc
     6421 tctgggagag gtgattgcgg caacaccttt aattgatgca atgctcaaca aacattggtc
     6481 agtgttagtt accacaatga cccctacagg ttctgaacga gtaaaatctc gatttggtca
     6541 taaggtagcg catcaatatt taccctatga tttaccttgg gttctcaaac gattttttaa
     6601 aagaacgaaa cctcgtgtcg ggatcattat ggaaactgaa ttgtggccta atttaattaa
     6661 tcaggctcat gcttcaggag tgtcattatt tctggcgaat ggacgtctat ctgatcgctc
     6721 cttgcaagga tatctaaagc tcaaattttt atttaaaccg gttttgaatc aattttcagg
     6781 ggttttgact caaagcaatg aggatgctga gagattcatt gccttgggtg caaatgccga
     6841 tttagtccat gtattaggga atatgaagtt tgatttgcag attgactctg ttgatagtag
     6901 tcaatacagg gaattaaaaa atcattgggg agaggataga cttaccgtta ttgcagcaag
     6961 cactcatgac gacgaggagt ctcagatttt atctcagcta cctcgtttgc aggaggctat
     7021 tccgggggtg gtgttattaa ttgctccgcg tcatccagaa cggtttcaaa cggtctatca
     7081 gttgagtgtt caagcaggat ttaatactgg gtgtcgtagc aatttggata cggtttccag
     7141 agaaaatgaa gtcgttatac tcgacagctt gggtgagctt ttgggatttt atcaaattag
     7201 tgattttgct tttgttggcg gcagccttgt cccggttggt ggtcataatg tattggagcc
     7261 aatcgcgatg aatgtgcctg ttttaagcgg gaatcaagtg cataacttta aatcaatttg
     7321 cagagagctt aaagaggctc aggcaatttt gctagtgaat catgccaatg agttggttga
     7381 tgcaattatc aagttatatc aggataagga atctcaaaac actatggttg caaacgccag
     7441 ttcagtcctt gaaagtaata agggctctgt agtgaggtat ttacagaaaa ttgagtcagt
     7501 tttgggatat tagcttgtta taaaattctc tccatattga tttcttgtat cataccagaa
     7561 aattcatcaa aacatttttg aacttgttca aggtggagta tccatggaca atggcctgct
     7621 ttataaatga ctttattaat cacattttga cattgaaaat tcttatcctg aataaatatt
     7681 gcaggtggac aaatgtaatc aagttcactg gcaatggtca tggctggaat ggtttgagga
     7741 tgccacttat agtgaaaatc attataaaaa tgctcgatgg cataatgata ggattcattg
     7801 ttgtaggcaa ataattccat catttgctca ccgattggta gctcttcccg agtgaaacaa
     7861 taatgcttat aagtatccca aaatttttta taggtttcgt tggatggata taaatgatat
     7921 tggctcgcag cagggactaa atccggcaaa ttatattttt gacgcatttc gttaacgtgc
     7981 tgaaagaaac tgttggttgt tgttgtattc attaaaacaa gcccgcttaa atgattctca
     8041 atttccggaa aattcagcgt gaacattcct gaaaaactat gggtaacaag gattggcttg
     8101 ttcaaagatt tcagcaatga aatgaggcct tcctgccaag atacaaaatc aagttggcct
     8161 tcactattat ttccatcttt tggaaaatca acaaggctta tagtgccctg caaagttagc
     8221 ttgtcgcaaa aactggctaa atattcagaa ccaagcccgg gcccacctgg cagaaacagc
     8281 cagtgttgat ccgagccttt tttgatttga tttaatctgt agccataatt ggtaaaatac
     8341 ataaaaaaca tcgattgctt gtaataataa actcccgcct ggcgggaatt tattgtttaa
     8401 tgttcagaga caagaaagaa tcaagcaccc tgattttggc gttccttgat ttcagataga
     8461 gttttacaat ctatgcataa ggttgcagta ggtcttgctt caagacgttt gaggccaatt
     8521 tcgattccgc aggcttcaca atatccaaaa tcttcatttc ttaatcgctc aagagcgtct
     8581 tcgatttttt taatgagttt tcgttctctg tcgcgggttc tcagctcaat gctaaactcc
     8641 tcttcctggc ttgctctgtc ggaaggatct gggaaattgg ctgcttcatc tttcatgtgc
     8701 gagacagtac gatctacttc ttccattaaa gattggcgcc aagcgagcaa tattttttct
     8761 atatgtgcca gctgcttttc attcatatat tcctcgccct cagtctcttg gtagggcgct
     8821 attcccatat ttcctattgc atcgtttttc acgttgtata atctctcttt ggttttatca
     8881 attaattgtc ctgtcctgcc agaccctata tttcattctg tatctgaaac tgagttttat
     8941 agaaaatcgc tacgtatacc aaaaaacgtc agtatcatca acaaaaaaat aatcactaca
     9001 aaggtttgtt attccgattg cttggacgtt gtcctccact taccctatca tgtccttgaa
     9061 gatcttgcca acagtgtatg tttttatatc ctaattgatt aagtatagtg cttatctcat
     9121 ttttttgttc aaaaccatgt tcaaccaaga gcagtccatc aggcaaaaga tattcataac
     9181 tgtgctgaat aatatattgt agatcagcta atccatcttg actactgact aatgcacttg
     9241 ttggttcaaa gcggacatcg ccttgtttta aatgaggatc attttcagca atgtagggcg
     9301 gattagatac aatggcgtgg tattgtttta gggggagatt attaaaccag taggaatgac
     9361 agaaattaat attgtttaag ccgagtgttt tagcgttata acgggctaac tccaatgcct
     9421 ctttgctgaa atcacaggcg tcaatatgcc aaagaggtct ttcttttgca agcgccaggg
     9481 caatagcacc acttcctgtt cccagatcaa ggacagaaac attctccttg tcaggtatta
     9541 gctccagtgc aagttccacc agacgctcgg tttcatgtct tggaatgaga acattgggtg
     9601 ttacttttaa cgaaagagac cagaattccc gctgtccagt aatgtaggct attggtagac
     9661 cttcagcacg ttgtttaatc atctgcaaat aggtttctat ctgttcagga gaaaccaagg
     9721 catccggatg ggcaaaaaga taggcccggt ttttgtttaa cacatggcag agtaaaagtt
     9781 ctgcctctaa cttggtttcc tggtttttga ctttgaattg ttgcagagct tgttccagca
     9841 atgaacgaat atcaatcatg tcgacctaaa tcggctagca gttcagcatg gtattcgcgt
     9901 tttaacgggt caatgacaag ggataaattc ccttccatga tgtcactaag ctggtagata
     9961 gtcagattga tacggtgatc agtgagccga ccttgaggaa agttataagt tctaatgcgc
    10021 tcggagcggt cacctgtacc aaccagtgac ttacgtgttt gagcttgttc ttgtttttgt
    10081 ttgcttactt cggcatctaa taaccttgtt tttaacaagg acattgcttt ggcacggttt
    10141 ttgtgctgtg aacgctcatc ctggcactct acaaccactc ccgtagggat gtgtgtgata
    10201 cgaatggcag aatctgtttt gttaacatgc tgtccaccgg ctccagaaga tctgtaagta
    10261 tcgatgcgta agtcatcggg atttatctga atgtcattaa tttcatccac ctcaggcatg
    10321 atagcaaccg tacatgcgga agtatggaca cgaccttgcg attcagtttc aggtactctc
    10381 tgtactctgt gagctcctga ttcaaatttg agttgtgaat aaaccgcttg cccactgatt
    10441 ttggcaatta tttccttgta gcctccatgt tcaccatggc tggcgctaat taattcaatt
    10501 tgccaaccct ggctttctgc ataacggctg tacattcgaa agagatcccc ggcaaatata
    10561 gctgcttcgt ctccacctgt gccggctctg acttcaagat aaatattgcg ttcatcatca
    10621 ggatctttag gaatgagatg ccattgtaat tgttcatcca attcctcaat ttgtttcttg
    10681 acggtgtcta tctcttcttc agccatagtc gctaaatctt tatcatcact ttccaataat
    10741 tcgtttaaag aatccaaatt attttttgcc tctaaatacg actcataaca ttgggaaact
    10801 ggttcaagtt gggcatattc ttttgataaa gacttaaatt gattttgatc ggcaataatg
    10861 gatgcttcgg acagtaagcg acctacttcc tcgtatcgct ccaacatttg ctgtaatttt
    10921 aactcaagag atttcttcat ataatgattg attggctgtt gtatcaaaga gatatcgtgc
    10981 gaggtcaagt aagtcttctc tgttatccca tgccatttgt cttaaaccta tggtgggatt
    11041 atgagttagc ttgttaacca agcgcatact gaattcattt aaaacgtctt gctgattttg
    11101 accagcggat atttttttca aagcacgttg taattcctgt tgagccaaag tgtgcatttg
    11161 attgcggtaa tcgcaaatga cgtctttggc tctaagcgag cgatgccatc taatgtagtt
    11221 atccagttcg ctttcgatca attgttctgc ttgtaatgcc gcatttcgtc tttcatccat
    11281 ccctttttct atcatagatt gtaaatcatc tacattatag agatgaactt gttccaactc
    11341 attgacattt ccttcaatat cacgaggaac ggccaaatcc aatagaaaca taggggcatg
    11401 gtttctttgc tctaaggcat gctcaaccag gcttttattg atgaatggga gcgggcatgc
    11461 tgtggcagaa ataacaacat cggccagagg taaatattgc gggatatcac caatggacag
    11521 tgtttttcca ccgaaagttt cagctaattt ttgagcattt tccagagtgc ggctcgctat
    11581 caaaaaacga tggacaccat gctgatgcag gtatttagca accagagagg ctgtttctcc
    11641 ggaaccaatt aaaaagacac ttaaggaatg atagttttta aataattgcc ctatcagttg
    11701 taccgcagca taggcaatcg atacgggatt agctccaatt ccgctacgag ttctgatgcg
    11761 tttggacgct ctgaaaatat attcaaacac aggccgtaat tgtgttttta ctgtgccaag
    11821 acgacaagca tgttgatacg cctgtttcat ttggccaagg atttgtggtt cgcctatcat
    11881 catggaatcc aaaccgctgg caacacgcaa ggtatgtttg attccttctt ttccctgatg
    11941 gatatacaaa aattgagata ataattcctc tgataattga tgttcatgag ctaaccagtt
    12001 tccaagaacc tcaggggtat tggtatcaca gtagatttca gtccggttgc atgtagataa
    12061 aatagccgct tcattgactt caggtaaatc aagcagactg ctcaatagag aatcttgcat
    12121 ggctggctgc agtgcgactt tttcacgcac atgaattgga gcagttttat gattaagccc
    12181 gcaagctaca aataccatag aatcttgatt tatttgttta agggaattaa cgaattgtaa
    12241 acgattcatt gtaaaatttg tagagaaaaa ggcaaggttc cttgtatttc tgtttcttgc
    12301 aatcatttta cagtatattt tttacccgcc ttcttcctca agattgtccc acttgggaaa
    12361 atttgctggc aagggattca atttactttc tctatcttca aacgggctca tatccaataa
    12421 tgagaacagt gactttctaa agtcctgttg agttttgccg ccaacatgta tagccacata
    12481 ttgcacaaca tgtgtcattt caataggagc tttaaatgga cattatcctg caaggtcaac
    12541 ataatgctat tgaaactgtt gagagcctgg agagggttct gaaattattt aaagagcgtt
    12601 atcaggttga tggatttcgc gagattcatt tgactgtcac tttagttgat gaacagggcg
    12661 atgaggtgga gttggttgat agtgatacga atcaacctta tcgaattttt gaagtttatc
    12721 gtaaaggata tgaattggct ggaagaaaag ggattccaat gttgcagctg gtagttgata
    12781 atacgcacaa atagattatt ctttctggtg tgtttttaat aagcctcata ttctggataa
    12841 ggagtccatt atgattatgg cattggtagc cataatactt acgttaatcc tggtgatagg
    12901 tatacacgaa ctggggcatg cactggcagc ccgcctgttt caggtcaaaa ttagtaaaat
    12961 ttccatagga tttggaaaac cattaattca atggcaaacg caaagtggtt gtaactggat
    13021 ctgggcaatg tggcctttgg gagggtatgt tcagttactg aattcgcgca ttagtccagt
    13081 aaagccacaa gaaaatgctt attgttttga taagaaacca atttggattc gtgtgctgat
    13141 tttattgtca ggagcgattg ccaaccttgt taccgcatgg atagccttgg tgttggtttt
    13201 ttatattggc attagctaca aacaacctca aattcaatcg gtaaaactgg atagccttgc
    13261 tgccaaggcg ggtattcaag caggcgacca atgggtttct gtagagagtt acccaactga
    13321 ctcctggcaa ggggtaggta tgcaattggt aattcattgg gggcaaaagg atgttcgcat
    13381 tgtcttaaga caagggaatc aacaattaaa acaattgaat cttgatttaa gtcagattga
    13441 atttacttca aaagatggct ctttgctcga gagtctaggg atcaaacccg atttgtcagc
    13501 agtttccagt ttgacgcgct atccatctct actggcttca atgcaaaaag cgtttgctga
    13561 gataatccat accatgtatt tttttataat gatattgaag caattgtttc tcggggttat
    13621 tcctttttca attttacttg gaccgttggc tattttttct gtttctgtgg cttctttaac
    13681 tcaaggcgtt atcgtttttt tactattcat tgctactttg agtcttgctg tagctttggt
    13741 caatttattt cctatacctg gccttgatgg aggcagcatt ttatattcgg ttatagagaa
    13801 aattcgtggg aaacctgtct cagtggctgt ggaagttttg cttcatcgct tgatgattat
    13861 tctattttgt gttttgttgg tgcatttatt aatgaatgat ttgaatcgat atttacacca
    13921 ttgacagtgg actcatagga acaggctgcc ttttagttct tagttacagc ccgcgcacaa
    13981 caacatatat ctactctctt aactccggca ttttttaatg tatgtgccat ttcattggct
    14041 gtgctgcctg tggttaataa gtcatcaaca atcatgacgt gttcataagt tacaggtggt
    14101 acataaaaag catgacgtaa attttttctt cgttgctctc catcaaggtt ggcttgtgaa
    14161 gcggtattga ttattttttg acaataatat aaatcataag ggatgtttaa ttgacgagct
    14221 aataatccgg ttaatacagc agcctggtta aatccccttc gttttaatcg tttgggatgc
    14281 atgggaacgg gaattagaca atctggtttc aatgcgcttt tgggtaaggc attaagaagt
    14341 aattgcttta gaaacgaggc cagataaagg ccattatggt atttaaactg atgtattaaa
    14401 ctacgcagag gctcttcaaa acgatacgct atataagctc tatcaaagga gggccttttt
    14461 ctaacgcatt gaccacaaac cagatacgta tcatcagaaa gggggtaggc acagtactga
    14521 cagcatgatc caatttgttt cataaactcc atgcaattag agcaaactgc aagttggctt
    14581 ttgtggaact ggttgcaaag agtgcatatt gaaggtaaac gtaagttctg tgttatactt
    14641 aagaattttt ggagcacgat agttggattt ccagcattta atttttccct ggtctttata
    14701 atgacggtca acaatgaaat aagcaaggca tttagcaaac atgccgatga ctatgagcgt
    14761 gtggccaaag ttcaaaaaga aatcggcagt cgtttgtttg agcgattaca atatttgaaa
    14821 attgctcctc gacgaattct tgatttgggg tgtgggcctg gatttttttc aaaagaattg
    14881 gctttgttat atccgaaagc gcagattgtt ggaatggatt tgtcgtttgc catgttagag
    14941 caagccagga aaaaacaggg ttggcgtcgt aaatggcctt tagtcagtgc cgatatgcag
    15001 aaaatgcctt ttgcaacagg cgtttttgat ttggtttttg ctaaccaggt tattcattgg
    15061 agtagctcat taggcacagt ttttcgggaa cttaatcgtg taatgaatgt caacggatgc
    15121 ttgatgttta ctaccttggg tcctgatacc ttcaaggaat tacaaacggc ctggtctgct
    15181 gctaatcaat atgctcatgt taatgaattt gttgatatgc atgatattgg agattgtctg
    15241 attgccgagc atttcatgga cccggtcatt gacatggaat tactgtctat acattacgag
    15301 acccttcccc aattgctatt agctctaaaa actcaagggg taagaaatat taaccctaaa
    15361 cgaaatcacg ggctcacagg taaaagtgca tggaaacaat ttgaagaaca atacgctact
    15421 atgcgtacta ctaccggcaa atatccctta acttatgaag tggtttatgg gcaagcctgg
    15481 aagggtgcgc aacgtaaaat ggagcaaggt atagaaacct ggattcctgt ttcacgtgtt
    15541 gttaaaaagt cctaatttaa aacggatatc aattggattg tccggtgcat caatggtgtc
    15601 aagacaaatt gctacgattt tgtaattgtt tggttaattc aattaatgcg gctgctttac
    15661 taccaaataa ccgcagagaa tccattgcag tttggtaatg aacagcgatt tcttcttcca
    15721 attgttgctt gttaaataag gttgcaaagg tggttttttg gtttgcctga tccgaggagc
    15781 ggccttttcc caatatttga gttggagcgt agagatccag gtaatcatct tgcatttgaa
    15841 atacaagtcc tatatgtttc ccataagttc ttagtgccga ttttatttgc tctgatacct
    15901 catgttgtgc tgctaaaacc atttcaaaac acgccaggat caattttcca gttttcaaaa
    15961 gatgaattcc tcttaattgc tcctcagtta ccgatgattt ggccagttct gataaatcaa
    16021 gactttggcc actaaccatt ccgcttattc cactggcatt taccaatact tgagtaatgg
    16081 ctacgacttg tgctgcgggg agaaggggcg ataatctcat cagtacgact tcaatcgcta
    16141 aagcttgcat gccatcgccg actaaaatag cagttgcctc atcaaatgct ttatgacaac
    16201 tcggcttacc gcggcgcaga tcgtcattgt ccatcgccgg aagatcatca tgaatcaggg
    16261 aatagcaatg tgttaactca agcgctgccg caataatatc cagaacttcc tggtcaacat
    16321 caattaaatc gccagctaaa taaaccaaaa tgggtcttat tcttttgccg cctgaaaata
    16381 aagcgtaatg aagtgcggag cgaatttggg gagcaggtat tactatttca ttgagcaatt
    16441 gctcgagata tagctcatgt ctttgggtat acttatcaat cacttgtttg ttcatctgag
    16501 tcgagttcaa tgttggaatc agtaccagtt aacgtttcaa ttttttgctc tgcctgattc
    16561 aaagcatttt ggcaacgtct tgcaaggcta attccctttt caaattgttt cagcgattct
    16621 tcaaggctaa gctctccctt ttccagttgc cggacaattt cttcgagttc ggtaattgat
    16681 tgttcgaaat gtatgccttt gctcataact gttttccaag gcgataaaag gtgtgattat
    16741 actgggttaa cgcgaggatt caacttctga atggaagtaa gtggaaaatc aatcatagaa
    16801 gcttattggc agctttgggt tttataaggg aactccagaa ctggatgttg gactttgcta
    16861 gtttaatatc aaagaccaac attaggtagt taacagcgtt taaagaccaa atgctttatc
    16921 aaaatccaga tcaaattctt catcctttgt tttgttattc tttgtagtga agaaagtggg
    16981 gctgtttttt atagatgtct catgttgttg aatctgatca cggcatttat cttttaacgc
    17041 aagcatttct tcatataagg agcgtatttc aattttcaag cgatcaacca cttcattgcc
    17101 gcctttgtaa atggtggtta gagaacccat tttgacaaag tgttctgtgt gggcgttttc
    17161 caggaatttt agttgagctg ttaaagagga ggactctttt gctttcgcta tttggtcttc
    17221 catatactct tttaatctgg taagttctgt taccggattt tgtccttttt tgaaagcaga
    17281 catgattttg acctcacatt ttaaaataat cccttaggta agttaaagca aaaacaggca
    17341 attgaaaagc aaatattttc atattctttt ttgttagata agtattaatg catagtgcct
    17401 ggtatagagt taatcaattt aaaatgaagt caaattgttt gtaagataag tctatggaaa
    17461 tcaggtgtga tttgtataaa aaacctgaca ttcgcattga aacattgtga agccaagcga
    17521 atgtcaggct cttgtctgcg gtttaattat ttgggtgatg aagccgcttg acgaagcccc
    17581 aaatcttctt cagctttttc taccgctttg gctgaatctg gacgtgcata agcgaagaaa
    17641 ccttgttgat aaccaaacgt aaagacggta atcaccgcgt tgcccaggca ctttaaaaga
    17701 tttttcaggt agtcacccca gcctaagtct ttttctaaaa ccggtttagc tatttttacg
    17761 gcatcttgac aagctaattt aaactttctt ccagcaagtt ccggagaaaa ttcattggct
    17821 cttaagtctc gctcatactc atcacgagca gctataagac tttggagaag tgtatttgct
    17881 gtgtcatgtg cttttttata ctggtgttga tcaactctgt ctattttgag tgtgagctct
    17941 tgaagaattc catcaacttt ctggagagct tgagttatag tttcttcctg ttgattttca
    18001 agcttaggtt tttcttcttc agattgctgt gcaagtttta gtttttcttc ttcaagctgc
    18061 ttttccagct tcaattgttc ttcaagctta agcttttcta cttcagcctg tatgcgttgt
    18121 tcttctaatt ttgtttgaac ttcactaacc agtgtcaatg gatgcttatg gctgaattga
    18181 tcacgtaaag gctcatagag ttttcttacg tcaattttta gctgttcaac ttgctcattg
    18241 ccacctttgt aaacttttga cagggaaccc attttatcat cgtattcatt caaagtgtct
    18301 atgagcagtt gatcgatgtc ctgttcagag gttgcttttt taatttttga aatttgctct
    18361 tccatgtatg ccttaagttc agttaaacct ttaactggat cctgacctac ggtgaatttt
    18421 gccataattt tatcctcggt ttttataaaa aaatcacttc ggcaaataat aaagcaagac
    18481 agtgtttttg aaaagtaaag ttttaataaa tatttcgtaa attgagtgca ataaaaagaa
    18541 aaaggggagt ttaagataag ataagttctg gttcatttaa aaattattta aggcaaatat
    18601 gttcagcgcg agttatcata aatgtactaa ataaaatgct tgaatcaagg caaaaattat
    18661 gaaagtgaaa acccagtttg tttgtaatca atgcgcggct atttttaaac aatgggcagg
    18721 acaatgtact caatgtggtg cctggaattc cattgttgaa gaagggatta tgcctgaaag
    18781 caagacttta cgaagtgggc aatgggctaa tcagcgctct gttatcactg ctgttgaaga
    18841 cgtcgtcatg gacaaaatag tacgtatgga ttgtggtata tccgagctca accgggtttt
    18901 gggagggggg ctggtttatg gctcggttgt tttgattgga ggggatccgg ggatcggtaa
    18961 atccacctta ctgttgcaaa cgctggctaa tttatctatg caagaaacag tattgtatgt
    19021 gacgggggaa gagtcattac aacaagttgc catgcgtgcc caaagattgg gactaccttt
    19081 ggctggattg cggctattag cagaaactca ggttgaatcc atcattgctc aagcccaaaa
    19141 ggaaaagccg cgaattattg tcgtcgattc agtacaaacg atattcactg aagccataag
    19201 ttctgctccg ggaggagtta gtcaggtccg ggaatcagca gcgcaattgg tgcgttatgc
    19261 aaagatgact aatacggcga ttttcctggt aggtcatgtc actaaggaag gaaccttagc
    19321 cgggcctcga gtgttagaac atatggttga tagtgttctt tattttgaag ggcaaaacga
    19381 cagtcgtttc agagttattc gcgccattaa aaatcgtttt ggcgctgtca atgaattggg
    19441 tgtatttgcc atgacagaca aaggtctgaa ggaagtggcc aatccctcag ctattttttt
    19501 atctcgccag cctgatccta cgccaggcag cgcagtgatg gtaacttggg aaggatcaag
    19561 accaatgttg gtagaagtac aggcattggt tgatgaggcc cacggccagc aagcgaaacg
    19621 cgttactgcc gggcttgagc ataatcgcct ggcgattttg ttagctgtct tgcaccgcca
    19681 tggaggtatt gccacttacg atcaggacgt ttttatcaat gtagtagggg gagttaaggt
    19741 aacggaaaca gcgagtgatt tagcattgtt ggccgcggtg atttccagtt tgcgtaatcg
    19801 tatatttgac aatgaaacca tcatattcgg tgaggtagga ttggcgggag aaatcagacc
    19861 ggtgcaaagt ggtcaggaac gcattaagga agctataaaa catggattca agcgtgctat
    19921 agttcctttt gccaatgctc ccaaacagca aatcgactcc atgacggtag agccagttaa
    19981 acatttgcga gatgtattgg agaaaatgtg agttatcaaa aatcaatcac actggaatat
    20041 aaaatgaatg aagatactat ccgtgatttt aatcgtaatg gtgccgtatg tcttcgccaa
    20101 tttttaaatg cggaagaaat tgctattcta aaagaaggaa tagaatttaa tttatcctcc
    20161 ttaagtcccc gtgccaaaat agccagcaga cccgatgatc ctggtcattt tgttgaggat
    20221 ttttgcactt ggcaaaacaa tcctttttat caacgaatta tctttggtac gaatttggga
    20281 gtagctgccg cacaaatcat gcaaagccaa acagtccggt tgtatcatga tcatttgttg
    20341 gtaaaggaac caggcacacg acaggtgaca ccctggcatc aggatcagcc ctattataat
    20401 attgagggca agcaaaactg cagtttctgg atcccggttg atcctgtcag ccgttcctca
    20461 actctggaat ttgtcgctgg ttctcatctg ggcccctggt taatgccaag aaccttttta
    20521 gacaatcagg caaaatggtt tcctgaagga actttgcagg aattgcctcc tatagaagaa
    20581 gcgcgtgcga agtatccgat tattggatgg gaaattgagc caggtgatgt ggtttgtttt
    20641 catatgctta ctttacacat ggcaagggga gttgagggaa ggcatagaag aagagtattt
    20701 tctgtgcgtt ttttaggtga tgatattaaa catgccccca gggcctggcc tacttcacct
    20761 gattttcccg gtttgacggc cgcaataccg gcaggcgcaa gcatggatca tccattattt
    20821 ccatgtttgc cactcaaaat atagtaaaac gatatatttt atttcccttc aaaaaagagc
    20881 tcattttttc aaggatgaga tttttaagat tcccattaac aacggatgtt attccaataa
    20941 gcgtacaatc aaaagggtat attttttatc attgactacc aaaattcgtt attgttttcc
    21001 attaagtatt gtattgtatt tttgaagtgt ctttatgtat acctgatcaa ggggcactgt
    21061 tcatggtaca tcggtttgct ctattaaact tatgtttaac cgtttgagga ctaatattac
    21121 aaggagtaat tatgacgtta cctacggagt taaaaagcaa gttcattcaa attctgaatc
    21181 aacacaaaat ttctcattct gttggttggt ctaactacat ggagagtgtt aataatccag
    21241 tcgcgattgt cgtggaagtt aaaaatgaaa aagaggttca gacagtgatg atggcgatta
    21301 aggaattgaa ttctgatagg gaacccaaag ataaagtgac tgtgcgagct actgcgggat
    21361 ggactgataa accgggtaaa agctgttgct tttttccatg gagcgaagcc caggagagtc
    21421 agtacaatga aggattttct ttttctcaag tcgtaggtgg taaagcatct cctgataagg
    21481 caggtaccga tgtcattatc cgttttgcaa aaaaatttca tcatgcaaaa gtgattggtc
    21541 cattaaaaaa tccccctgtt tttaatccag acaatcccat tcatagactt cctgtgactt
    21601 tggtggaagt gagtgcaggg atgcaagtgg ctgattttgc cgatttttta cgagaacatc
    21661 agttatcctt gtcaactgtt agcatgattt cctgggtgac ccctgttggt ttggctggaa
    21721 cagcgggaca tggtaccggg cgtgatgaac cggctttcag tggcctaatt gaatcggtta
    21781 cagtttgtga tatggatgga aatctgcgtg aaattaattc cactcatcct gatttcaaag
    21841 atttgatagg tggccatagc gggttgttgg gtattgttgt tagcatgaaa ttacgagcgg
    21901 ttaaggcatt taatttatgt gaaacaatag aattatttcc caataccagg gaaatgacag
    21961 gcaggttaag tgatatatta aagaataatc aatacatcag tattatggga atgccaagtt
    22021 acggctgtcc tgaatcggat aaactggttt ccaaatggca aattcgtcaa tggaattata
    22081 ctaccgacaa accaggaaaa aaggaaaagg caccttatga tccggacgtt cgctcttttg
    22141 ctcaagagtt acaggttcgc attggtgcct cagtgatgga tttcttactg gactcaggat
    22201 taaagcattt attaccttct tttatgttac tgtcagcagc agtagtcaca ggtactcgtg
    22261 gtacatcacc aaaagtagat tatgaaaatc atatcacaca cccccaggtg gcttttccaa
    22321 aggagatgag agatgtgagt tatttgattc cagtcaaaga taatgaagca gggcaaaaat
    22381 tggaacttat tttacaaaaa atggaagcca tgttgaacga agcgggtagt aagggggaat
    22441 ttcctgttac ttatgcgatt tatgttcgtt atcttaaggg gactaacgga ggattatcaa
    22501 ccagttttac ttcatcagat gatgagcgta ttttagcgat agacgtagtc actcatcctg
    22561 aagcgcctgg gattacaaat tttgagaagg gattcatgtc ttttttaaaa gagatgaata
    22621 ttatccccag aaatcatttg ggaaaaaatt tccccgcggg cgtagtgcat tatgatcagt
    22681 ttttgggtgc ggagaaggtg tcaaagtata agcaggcact ggagagattc tattgcagtc
    22741 caggaaagaa cgatggtgct gagcgcctga gcatgtctcc tttttatacc ccttacttgc
    22801 aaagcatgtt atctgcaagt gcacctacca atcaattagt tgatgaaaat aaatacgagc
    22861 aacgcataga gaggttgcat gaggagcatt ctgatctcga atgtgctgag tttttgagta
    22921 aattatatgc cgaggtgtcc aggatgagta cgttttcaga agaggctctt actgcaaaaa
    22981 aagggttttt agaagcatgt aaggcggaat tggagcacag gaacagtcga ttagaagccc
    23041 ctgtacctct attgagttaa gcaatcagga aaataagtac aagacagagc ctagcaaatt
    23101 caggagtctc tactaaactt aaaaatataa taatattttg agttaaccta tggatattac
    23161 tccttttttt aagctcatgg tcgataaagg tgcctcagat ttgtttttca gtgtcggggc
    23221 accaccaaat attaaaatcg agggcatcac atcacctatt ggccaggttc ctatcaaatc
    23281 gaatcaaatg gcagaaattg ttttatctat tatgaatgac gcacagcaaa aagaatttga
    23341 gtctaaaatg gaactcaata tggctttttc attagctgga gtagggcgtt tccgaatcaa
    23401 tttattcagg cagcgtggcg aagtggctat ggtggtgcgt tacatcaagg gcgaaatacc
    23461 ttctatagaa gatttaaatc ttcctcaaat tttaaagtct ctggtcttgg agctgcgagg
    23521 tttagtattg gttgttgggt ccacgggatc gggtaaatca acgactctgg ctgcgatgat
    23581 caaccaccgt aatgaaaatc acagagggca tattttaaca atagaggatc ctattgaata
    23641 tatacatcaa cataaaaaat caatagttga tcaacgagaa ataggtattg atactctaag
    23701 ctatgacaat gcattgaaaa acgcaatgag agaagcgcct gatgtgatat taattggtga
    23761 aattcgtgat cttaactcaa tgaaacatgc gatagcttat gctgaaacgg gacatttgtg
    23821 tatttctact ttgcatgcca ataacgccaa tcaggctctg gatagagtga ttaatttttt
    23881 ccccgatgag gcaagacaac agatattatt agatttatct cttaatttaa aagcaatcat
    23941 ttcattacgt ttgataccag gagttgataa tcaacgtgtc gcagcggtgg agatcttgat
    24001 taactctcct tacatttccg atttgattga aaaagggaag atagacgata ttaaagaagc
    24061 catggcaaga agcacggaac aaggcatgca gactttcgat caggctttat ttaacttata
    24121 taaagaagga aaaattacca aagaaaatgc gattaagtat gctgattcaa aaaataatgt
    24181 gggtttacaa attcgattgg gcgaagaaaa aagctttaat attcaagatg agaatttaca
    24241 aatacaaccc gatgatattg ataaggtttg attcattgtg aattaatttt gaacattgtt
    24301 ttgaatgaaa cattgtgata aaattatagt ctaatttgtt aatttttctt ccattccatg
    24361 cctagagttt ataatcttaa agatatttat ctgggtgccc cttctttttc agggcatgaa
    24421 gtttatcttg atgcagttta ttatccttct gatccgtccg aaaaaaattt cagggttatt
    24481 tataagaaaa acaagtttgg taacgctaac ttatctcgca tggaagtggc ttttagtcag
    24541 ttagcccgat tatttttaga taatggatta acttcctttc aaaaaatggt agtgaatgat
    24601 gcaaataaag tacagggatt gatagttgag catttaaatt atgtgattga aaataaagaa
    24661 ggattgaagc agccatttta tactttgaat gcaccaagga atggatgtga ttttactgaa
    24721 aagcgagtga ccagcccgaa tgagatacct ttctatttcc tggataagtt gcctcaaggt
    24781 ttttttaatc aattacttgc ggcagaaaaa aataataaat tgagcataga ttatgcctct
    24841 ttggcaagta tattggctac ttcgtacact ttggaagaag acgatttgca taaagggaat
    24901 tttggttttt atttggtgaa gaaacaggga aaaccgcgag ttgttttttt caaaattgat
    24961 catgatttaa tgtttgtaga cagcatcatg tcatttacaa caagaagatt ctgccatttg
    25021 tttgatggat gtgatgcttt tgatatcaca gaagaagatt tgttaaagtt tcctaattta
    25081 aaatattctg ctaatggtta ttggccaacc aaaaccagca ttttttataa accatgggat
    25141 aataaagatt atcgtaccta tgctgaaatt caggcttttg cggatttatc ccatgtggaa
    25201 gagtttaaca aggcaaaatg gcggtctttt tacaagcata ttttaatctc tcaatctcaa
    25261 atggaggcta ccttaaaggc ctgctttgat gagaataact cttcagacag ggcacatatt
    25321 tctctggtaa ttcaagcgat gttagccaga caggcgcgtt taaaggcgat gttattttct
    25381 ctaaaagatt ttcgtgattt tattctttcc caaaatggaa aagagagaga tttattatgt
    25441 catgaaatcc tgaataactt gccagaggaa gagagaaaat cttttgaaaa tgaaattaga
    25501 caatcgttag attataacca taacctctgt tgttccggtc tttttgagga tggggatacc
    25561 ccgctgcata tagcaatcaa gtcaggtgat tatcgttatg acgaaacgat tggcatgtat
    25621 gggcaattca tcaacacgaa aaacagctca ggaaaaactc ctttggatat cgctttacag
    25681 atggctgggc agtctaaagt tcatcctgcg gatgtgcgta aagattatcg ttttatcatg
    25741 aaacatttat tggcgaatgg agctaatcag acaaaacagt ttgaagaatt cgataaaatt
    25801 gaaaacatca gatcctatca atttcatact ccttatctaa acaaagccat aaaggctaaa
    25861 acttatcatg aactgaaaga agtactcagg gacattggtg aagatcatca gtattgtttg
    25921 aaattcaaaa agatgttggc agttgaatgc atttctgaat ttataaaggc caatcaagat
    25981 aatctcagtt taagaggaat actgttaaaa ttaaaaaagg aagtggatgg taagggaaca
    26041 aaatcagaaa atgcggcttt aatgtatata cgtcaattaa gaagtcggct atggattgta
    26101 cgtcaaatca gaggacttta tggctggtct acaacccaag gcgaaattga ttatatgatt
    26161 gataaagagc tggcgcgttt ggatactaaa gatttaaaga gactttcatt atttgattcc
    26221 agagacagca gtactttgga taatgtcttt ttggatatat cactatcaaa gaataaaatc
    26281 taagttcatt ctattgagaa aaatacc
//
LOCUS       DBNCWK010000029        26210 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.029, whole genome shotgun sequence.
ACCESSION   DBNCWK010000029 DBNCWK010000000
VERSION     DBNCWK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26210
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.029"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1418
                     /gene="ceg25"
                     /locus_tag="AC06IO_001116"
     CDS             <1..1418
                     /gene="ceg25"
                     /locus_tag="AC06IO_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg25"
                     /protein_id="HIO9874319.1"
                     /translation="AFYNSIPLIGKHLKSESRAKKYTSEEFKRLEREYRDHIGLPENS
                     TFSSDAFDKLVSSRVDDFRAFPKTNLPGGLLFHDKLKEANTTEGDNIRDVLSKTVLAP
                     TEKFKTAQKNFTESTSTFKELTKLIPSKFRAQDLVGTMKELTDDARKAIIEQQAHEIQ
                     MLKEKLDPQKEGQAGNYVSDFKKALGLKDNDEVKKVTEGLIKELEETHKKQLGEFDKS
                     TSESLNQLHKASASESSEYLFLANLYENNKEMRQTLEKLYAKERQRKGLPPESTTVQV
                     GISENKIDLNGIKLKDIPVIKSITGREIHQQPDGSFAIQMPMINPFYYQDPRQNVKTD
                     MMLIAQAIKASGYDSIEMNCNFPNSEKVAMERGRQAFAACIEAGFPVDKITINVNGKP
                     MKADELFKEHPKTYQDIMSRASKISKDYEDIKAPKTSTAPVNTVVVKQEIAALRAKQQ
                     QEERAQTQPVDEQRHGMTPNN"
     gene            1690..1857
                     /locus_tag="AC06IO_001117"
     CDS             1690..1857
                     /locus_tag="AC06IO_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA3496 family putative envelope integrity
                     protein"
                     /protein_id="HIO9874320.1"
                     /translation="MSTRRFDEFDNEMDDFSDAEPVITEASPSTKLERRRKIEDLLEE
                     KRLREELEEFG"
     gene            complement(1854..2585)
                     /locus_tag="AC06IO_001118"
     CDS             complement(1854..2585)
                     /locus_tag="AC06IO_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9874321.1"
                     /translation="MNRIKAVGYENNELKEKAQLLADQLNLQLDQNADTCLFVTSEKL
                     TLKIRNFSLMFADFSAMTWSKRRGEGKRQGLIRACKPTKGIKILDATAGWGKDAAILA
                     TFGADVLMLERHPVMAALLTDALSRRNEADIQKMCLSLIASDAISFLHSLQEKDYPDI
                     IYIDPMHPERNKSALVKKEMQVLQQLIGTDHDAMELIELSLSHVKSRVVVKWPQKVKP
                     LLPPDASIDGKTVRFDIYMPQFSSN"
     gene            complement(2588..3118)
                     /gene="gmhB"
                     /locus_tag="AC06IO_001119"
     CDS             complement(2588..3118)
                     /gene="gmhB"
                     /locus_tag="AC06IO_001119"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIO9874322.1"
                     /translation="MNRIILLDRDGVINTDSINYIKNADELIIIPDSITAIARLCAAG
                     YKIGVATNQSGVSRGLYSEDDLSDIHCKLREVLAEAGGQIDALEYCIHLPEDNCFCRK
                     PQPGMLKALAEKLNCSLDSVPFVGDRVSDIQAALAVGAQPIIVLSNMTDMSELAQYPK
                     VPIYNSLLQYVNQLLL"
     gene            complement(3124..5190)
                     /gene="glyS"
                     /locus_tag="AC06IO_001120"
     CDS             complement(3124..5190)
                     /gene="glyS"
                     /locus_tag="AC06IO_001120"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIO9874323.1"
                     /translation="MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEV
                     KRFATPRRLAIVIHDLQTEQESQKVMRRGPAAIAAYDSEGNPTPALLGFAKSCGVTVD
                     ALTKIQTDKGEWIVFETQSEGTKTKDLLPGLVSLSLESLPIAKPMRWGEGDDEFARPV
                     HWAVMLYGSETLNHKILGVTSGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFA
                     TRRQAIIHQVQELAARLGASVVMPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALI
                     ASMQSHQKCFALKNKYGELLHYFITVSNIASSNPKQVVLGNEKVMRARLSDAAFFFKQ
                     DKKQPLSAHIPSTEQVIFQVKLGSMYDKVQRIKAMMNHLSQSLNLPSHLAERATLLSK
                     CDLMTGMVGEFPELQGIMGYYYALNDGEEAAVATALNEQYMPRFSGDDLPSTDLGKAL
                     SLADRIDTLVGIFAIGQKPTGVKDPFKLRRHALAVVRLLISISAPLNLSTLIKEALAN
                     YGDRLPQDKNLVAELKPFILERLQSYYQNQGISADLVHAVRARQDDWLYDLDKRLFAL
                     KSFITMPEATSLSAACKRVGNILAQAAYADKKVDIKEQLIEEGAEKALFDHLNKITKT
                     VETLYIAGDYQALLKLLASLKEPVDAFFDHVMVMVEEESLKQNRLALLQRLQELLQGV
                     ADISML"
     gene            complement(5180..6085)
                     /gene="glyQ"
                     /locus_tag="AC06IO_001121"
     CDS             complement(5180..6085)
                     /gene="glyQ"
                     /locus_tag="AC06IO_001121"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIO9874324.1"
                     /translation="MTKPTTFQEIILTLQKYWSDLGCVILQPLDLEVGAGTFHPATFL
                     RAIGPEPWSAAYVQPSRRPTDGRYGENPNRVQHYYQFQVVLKPSPLDIQDRYLDSLRA
                     LGVDPLLDDIRFVEDNWESPTLGAWGLGWEVWQNGMEVSQFTYFQQVGGLVCKPITGE
                     LTYGLERIAMFVLGVDNLFDLPWCKTANGIVTYGDVFHQNEVEMSAYNFEHANVEELF
                     KQFDYYEGEAQKLIALQLPLPAYEMVIKASHTFNLLDARKAISVTERQRYILRVRHLS
                     RAVAQAYYQAREALGFPMQKVCNGQ"
     gene            complement(6320..7105)
                     /locus_tag="AC06IO_001122"
     CDS             complement(6320..7105)
                     /locus_tag="AC06IO_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIO9874325.1"
                     /translation="MKFTSLLTAGALASTLVSPAIMAADTASASLSDAQKKEIEKVIH
                     DYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLV
                     EFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQ
                     AMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLM
                     GTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN"
     gene            complement(7313..9478)
                     /gene="uvrD"
                     /locus_tag="AC06IO_001123"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(7313..9478)
                     /gene="uvrD"
                     /locus_tag="AC06IO_001123"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HIO9874326.1"
                     /translation="MTIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIA
                     WLIEEQHLSPHAILAVTFTNKAAGEMRSRLSSMLSTPTLGLWVGTFHGLCHRLLRRHY
                     KEANLPEQFHILDSEDQARVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHIN
                     ALHYGPTKTLVSIYKAYEDVCQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAIL
                     VDEFQDTNTIQYAWIRLLAGDHTAVLAVGDDDQSIYGWRGAKVENIQQFIHDFKDTQI
                     IRLEQNYRSTAMILNAANALIHNNSTRMGKELWTAGSEGEKILVYSAFNELDEARFVT
                     ERIGMELNQGASADEIAVLYRSNAQSRVLEEALLRAGIAYRIYGGVRFFDRAEIKDTL
                     AYLRLLVNPNDDTAFERVVNFPTRGIGEKTLDEVRQYARAEQCSLWGASKGILQSTGL
                     GQRGSLALAKFIDLIEKLQVVVANKELDEQISDVIQHSGLYAHFSKIKGDKSESRVDN
                     LQELINAAKQFRYEYDEEEKLPLVNSFLAHASLESGELQADEHERSVHLMTLHAAKGL
                     EFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLCYVGMTRAMRKLVLSYAEVRRQYGR
                     EEYHRSSRFLREIPQQFLDEVRVKSRSQWPGTTKSKLPVADEVSGITIGQNVQHAKFG
                     QGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTECL"
     gene            9699..10658
                     /gene="pip"
                     /locus_tag="AC06IO_001124"
     CDS             9699..10658
                     /gene="pip"
                     /locus_tag="AC06IO_001124"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HIO9874327.1"
                     /translation="MHTLYPAIKPYTQHELKVSKLHTLYLEETGNPEGIPVIVLHHGP
                     GGEADPHLRRFFDPQRYRIILFDQRGCGRSIPHLEISENNTQFLMEDIDSIRDFLGLR
                     EFVLFGGGWGSLLALLYAQKFPQQVKALLLHQIFLGRQKDIDWFYKHGASLVYPDYWE
                     EFTNTVPENKLDNIPKYYAECLQGPNELARMAAAKNWALWQARCSSLQPHLYVIDQYS
                     DPHFALALATLESYYIINHYFIEENQVIANAHKIRHIPTYIVHGRFDLVSPLSSAWEL
                     HQAIPASNLRIVRDAGHSDRESGIIDALIYASKDISKQGLDAC"
     gene            10652..11089
                     /gene="dtd"
                     /locus_tag="AC06IO_001125"
     CDS             10652..11089
                     /gene="dtd"
                     /locus_tag="AC06IO_001125"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIO9874328.1"
                     /translation="MLTVLQRVKEARVDIDGQTVGKINHGLLILCGFEPNDSLENIKR
                     MLDKCINYRIFEDPSGKMNLSLKDVNGGLLLVPQFTLMADTQKGLRPSFSNAASPELG
                     RELFDNLLTLAQKCHQNTQSGCFGANMQVYLCNDGPVTFLLQF"
     gene            11306..12088
                     /locus_tag="AC06IO_001126"
     CDS             11306..12088
                     /locus_tag="AC06IO_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIO9874329.1"
                     /translation="MRLILMFASIMGSVILASCASKGSNPIDPFEPFNRKVHNFNMAF
                     DATVLRPPAKLYQAVVPKLVRKGVNNAFNNLDLFPTIASDILQAEGKWVIKDGWRLFI
                     NSTIGVAGLFDVAEKFGLPPHYNDLGLTLAKWGDKKSPYLVIPFLGPSTLRDGAGWLF
                     QFALWSPYVYIDDAGLVYGLIGLRYIDLRTQLFDSEPLLNEALDKYTFIRDAYLQHRN
                     YLIAGTEQDTGASFVEDAKANKTAMQESSASNNQLGSDYVDE"
     gene            complement(12200..13159)
                     /gene="gshB"
                     /locus_tag="AC06IO_001127"
     CDS             complement(12200..13159)
                     /gene="gshB"
                     /locus_tag="AC06IO_001127"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIO9874330.1"
                     /translation="MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTQNDLYC
                     CDGSAYARVHEIHIGDIESLSWVKTIDLGNKPLRDFDVILMRKDPPFDMEYIYATYAL
                     DLAEQEGVLVANKPQSLRDANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFK
                     PLEGMGGNSVFYVDKEGKNLSVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGD
                     PVPFALARIPAKGDLRGNLAAGARGEVVAITERDKWICEQIAPVLREKGLYFVGIDVI
                     GDYLTEINVTSPTCIREISTETGLDIAGEYMRCLEKVHASLSK"
     gene            complement(13159..14454)
                     /gene="gshA"
                     /locus_tag="AC06IO_001128"
     CDS             complement(13159..14454)
                     /gene="gshA"
                     /locus_tag="AC06IO_001128"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIO9874331.1"
                     /translation="MNKHEIPVPHLTTAHSGPLHHVEKIILNKLAEIESWFRKKWQET
                     PAPLTSSVDLRHAGFKLAPVDTNLFPAGFNNLNPEFLPLCIQAAQSALIEYIPDCTKI
                     LLLPESHTRNKYYLQSLNVLKTIFVKAGFIVRIGSLDPEIKEPTEIILEQGETLLIEP
                     LQRQGDKVGLDNFSPCLLLLNNDLSSGVPEILQDVQQRIRPTAKLGWASRLKSSHFQF
                     FEEVAIEFAELVGIDPWLINPYFSAIEGVDFMAQEGIETLAQEVDKILSLINDKYTTY
                     GIENKPFAVVKADNGTYGMSVMMVHNGDEIRQLNRKQRTRMSTSKGSRKVDRVIIQEG
                     VYTFETMPDGSVAEPVVYMIGQFVVGGFYRVHKGRGIDENLNAPGMHFEPLAFAQACN
                     MPCDDLEVVDCPNRFYVYGVIARLAALAAAREIAAIGGE"
     gene            14833..15729
                     /locus_tag="AC06IO_001129"
     CDS             14833..15729
                     /locus_tag="AC06IO_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIO9874332.1"
                     /translation="MLHLKTIQSILLLIVLGFIWGSGYTLAKFAMTNGVNPLGYAFWQ
                     SLGPALLLTLVSLFTKQARLLHPVYWPYFFICGLIGIAIPNTNMYFIASHIPAGLLAV
                     LVNTVPLLVYPLALIAGQEKPDGWRFFALLVGMSGILAIIGINSAGLYSSWTILAMLS
                     PLGFAFCSIYIGAKQPKEINALQAANGMLLAASLLLTPVVLKQHAFYSLSAPLTLVKQ
                     IVILEIILSSLGYLLFFILIRMAGPVFYSLTGGVVAITGLFWGYLAFDEKPSSIQSMA
                     IALVVSAIFLLSWRQSKQTQGI"
     gene            15733..16014
                     /locus_tag="AC06IO_001130"
     CDS             15733..16014
                     /locus_tag="AC06IO_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874333.1"
                     /translation="MLSELAKQFSTQIQTFFYLIMLINGLLHFIFAGAVARDAGNLYR
                     LGQKPVLVSAPTWAFATLIGGVITATIYWLLHHSTLTRPTMREIRYDKA"
     gene            16001..16351
                     /locus_tag="AC06IO_001131"
     CDS             16001..16351
                     /locus_tag="AC06IO_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIO9874334.1"
                     /translation="MTKHKIKTIDVHELKHQMDNQSNLSLIDVRELEEWEMMHIPGAL
                     HIPKDRISTEIQNQIPNKEQTIYLHCRSGVRSLYAAQCLMDLGYYEVYSVDGGIMAWA
                     MSGYPVKQESYTPS"
     gene            complement(16385..17047)
                     /gene="lem14"
                     /locus_tag="AC06IO_001132"
     CDS             complement(16385..17047)
                     /gene="lem14"
                     /locus_tag="AC06IO_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem14"
                     /protein_id="HIO9874335.1"
                     /translation="MSFELVAYEKLKGSIRESIITLIKSHNEKAKIIEDKLEDSVKEV
                     SRERQPQVLVLLKTIELLDHSSKEPEDKARVLNALAYYIRDQIAATYKYTSPDNSDFY
                     KSLTISLDLNKDNNPNREDLADMYGALEKFLRSHVYKNSDPRKGYLDKQPFAIKHYSV
                     VDDILELSDRVHKLRREIIIAARDLHLLQQKPKSSQGGLFSVPSGKEKVTETQEALTH
                     RL"
     gene            17418..19055
                     /locus_tag="AC06IO_001133"
     CDS             17418..19055
                     /locus_tag="AC06IO_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIO9874336.1"
                     /translation="MKKISCSILLLINSLISYADSDQPKLILQIVVDQLRGDLIYRHH
                     KQFGPSGFNYLLAHGLDYHNAHHPHANTTTCAGHSTIATGSYPSLHGIVDNDWYDRKT
                     RKLKYCMEDLQAKILPTVHTHIEISGRSPRNLKPSTLSDEIILANKGKAFGVSLKDRA
                     AITLAGHAGKAFWFDKTNGGFITSNYYYYSYPLWVENWNKLYQPKAYDWNLSHPKSFY
                     QNANTLPFRHNYGSFGQTFPHHVTNPPSEEYFKSLSRTPKADELTADFAKQLLVNEKL
                     GKTTGQTDYLAISFSAVDAIGHQFGPNSLEAEDNLIALDDTLSQLFKTIDKEVGLNNT
                     LIILTADHGVSDGPSYLKANKIPEIKPVNIEATGKHIENLLLKRFNLPTQALMSINPP
                     FIYLDHQIIKEKNLDIAEVSFYLAEEISHLPGIFKAYPLPIRDIEKDWLSAKVDRMSY
                     AYRAGDVYIVQPPYQSHGAKSEDRVAHGSPWQYDTYVPLLFVHPDFKPKRIFRQVHTT
                     DIAPTLSSLLLIKPPAAAVGQPLVEVVNAFHKTGEND"
     gene            19108..19746
                     /gene="udk"
                     /locus_tag="AC06IO_001134"
     CDS             19108..19746
                     /gene="udk"
                     /locus_tag="AC06IO_001134"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIO9874337.1"
                     /translation="MTKQAIIIGISGPSASGKSLLANTIVNELGSEQVVVISEDAYYK
                     DNGHLPFAEREKINYDHPDSFDHALLYEHLRQLRVGNTVQIPIYSHSKHLRLPETRAV
                     GQHAIIVLEGILLFSDKALREIMDIRIFMSTPLDVCLTRRLKRDVVERHRSFESVIHQ
                     YETTVRPMYMQFIEPSSRYADIIVPRGGENRIAIEMIQAKMRELLASHTRGS"
     gene            19842..20648
                     /locus_tag="AC06IO_001135"
     CDS             19842..20648
                     /locus_tag="AC06IO_001135"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIO9874338.1"
                     /translation="MGGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFT
                     YQNEKLQSRVEAMASEFNSTLVFPCDVASDEEIKAVFDNLRNHWDKLDILVHSVAYAP
                     ADQISGDFVECANREGFRIAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAV
                     PNYNVMGVAKASLEASVRYLAASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYA
                     NITPLQRNVTADEVGNTAAFLCSDLASGITGEVLHVDAGYHAVSAMSELG"
     gene            complement(21182..23056)
                     /locus_tag="AC06IO_001136"
     CDS             complement(21182..23056)
                     /locus_tag="AC06IO_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIO9874339.1"
                     /translation="MLQKLNEHIQGVVAWLVIILIAITFTLFGVDYYFQSRQISDAKV
                     IVNDKPITMQAFETNYRRTRAQQDLPQMTAADEKNLQNQVLNQMITNEVSIQAARKYG
                     FEVSPEQANAAIVQIPQFQEDGHFSAQRYQQALSGALFTPETFQKEVRQGMLLNQQRF
                     AFMGTSFALSDEIKRFVRLYMQTRDYDYLTVPSDRFEQQAKISEDDIKSYYNQHHKKF
                     MTSEQVTLDYVLLSMHDIKSQIKISPDEIKSYYEENKSNYLTPAQWRVAHILFAVPEN
                     ATKEEQDSIKQKADEVYSDLKKHPEQFDKYVASKSDDKLSIANKGILPWITGGQNEYD
                     RALSNLTRPGQISIPVQTKYGYEIFKLIAYKPVTTKSLQEVENIIKDQLTSDMAQAKY
                     AQVLEQLTDLSYQTPDSLDPVLDSLKLKIQHTEPFSRDGGKQPITKNKQVIHAAFSTD
                     VLELGNNSEPIQIDNDSVIVLRVKKHIPSREQTLDEVRDQIKKILAKQYGDAKAKEIG
                     SSLLHPVEDKQQQELIANNQFEWHSVENASRDSDKANSLINDLAFNLLRPESRDGVIL
                     DNGDYVVVKLKRINDGKLSSLDQEQRDSLIQQIEASYGMMDYDLYVNNLLHHAKIERK
                     "
     gene            complement(23148..23224)
                     /locus_tag="AC06IO_001137"
     tRNA            complement(23148..23224)
                     /locus_tag="AC06IO_001137"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23188..23190),aa:Asp,seq:gtc)
     gene            complement(23231..23306)
                     /locus_tag="AC06IO_001138"
     tRNA            complement(23231..23306)
                     /locus_tag="AC06IO_001138"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23271..23273),aa:Val,seq:tac)
     gene            complement(23314..23592)
                     /locus_tag="AC06IO_001139"
     CDS             complement(23314..23592)
                     /locus_tag="AC06IO_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIO9874340.1"
                     /translation="MNKSELVDAIASGSGLTKADASRVLETFMATVTDVLKKGDQVVI
                     PGFGSFSTGNRSARTGRNPQTGKTIQIKASRVAKFKAGKNLKEAVQQA"
     gene            complement(23721..26171)
                     /gene="lon"
                     /locus_tag="AC06IO_001140"
     CDS             complement(23721..26171)
                     /gene="lon"
                     /locus_tag="AC06IO_001140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIO9874341.1"
                     /translation="MSNENEIISNETVKSSALPVLPLRDVVVYPHMVIPLFVGRGKSI
                     KALEAAMIDNKQIFLVAQRKSAHDDPGPEDIYQVGTVSSVLQLLKLPDGTVKVLVEGE
                     QRARVKEYTQDKGYLEATLEYIEEVGSTIQEQEIGILMRSLMSQFEQYIKLNKKIPPE
                     VLSPLAGIEEPGRLADTIAAHLTLKVDDKQELLETMDVGTRLEKLMAAIENEIDLLHV
                     EKRVRGRVKRQMEKSQREYYLNEQIKAIQKELGEMGEEGNEIEQLENSIKKAGMPKEA
                     KEKSLAELHKLKMMSPMSAEATVIRNYLDWMLMVPWKKRTKIQFDLLKAEKLLDKEHH
                     GLEQVKERIIEYLAVQQRVKRLKGPILCLVGPPGVGKTSLGQSIANATGRTFIRIALG
                     GVRDEAEIRGHRRTYIGSMPGKIIQKLCKAGVKNPLIMLDEVDKMAMDFRGDPASALL
                     EVLDPEQNHTFSDHYLEVDYDLSDVMFIATANSLEIPAPLLDRMEVIRLPGYTEDEKV
                     SIAEKYLVPKQMLLNGLTNEEIHISEGAIRDIIRYYTREAGVRNLERDIASICRKVVK
                     EILSSKKGKKGKTISITVSNITKYLGVKKYRYGLAEQYDQIGQVTGLAWTSVGGELLT
                     IEASIMPGKGKVTHTGQLGEVMQESIHAAMTVVRSRAKAFGLPDDFYEKHDFHVHVPE
                     GATPKDGPSAGIGMCTVLVSIVTQIPVKADVAMTGEITLRGQVLPIGGLKEKLLAAHR
                     GGIKHVIIPEENVKDLEEIPDNVLRKLTIHPVNTIEQVLELALQRNPWVEEPTNIQPG
                     TILNKRSKKIKNNDLHAH"
BASE COUNT         7935 a         5524 c         4643 g         8108 t
ORIGIN      
        1 tggcttttta taattctatc cctttaattg gtaaacattt aaaatcagag agccgtgcaa
       61 aaaaatacac ttctgaagaa tttaaaaggc tagagagaga atacagagat cacatcggtt
      121 tgccagaaaa ctcaaccttt agttctgacg cattcgataa gcttgtcagc tccagagtag
      181 atgatttccg ggcttttccg aaaaccaact tacccggggg tttgctgttt catgataaac
      241 tgaaggaagc caatactacc gaaggggata atatccggga tgtcttaagt aaaacagtcc
      301 tggcaccaac tgaaaaattt aaaacagcac aaaaaaattt tacagaatca acttcaacat
      361 ttaaagagct caccaaatta attccaagca aattccgagc ccaggatctt gttggcacca
      421 tgaaggaact gactgatgat gctcgtaaag ccataataga acagcaagcg catgaaatcc
      481 aaatgttgaa agaaaaattg gatccccaga aagaaggaca agctggtaat tacgtatccg
      541 attttaaaaa agcactgggt cttaaggata atgacgaagt taagaaagtt acagaaggct
      601 tgattaagga attggaagaa acacataaaa aacaactggg tgaattcgat aaatccacaa
      661 gcgaatcgtt aaaccaacta cataaagcct ctgcgagtga atcatcagag tatctgtttc
      721 ttgccaatct gtatgaaaac aataaagaaa tgcgtcaaac gcttgaaaag ttatatgcca
      781 aagaacgcca aagaaaaggg cttcctcctg aatctacaac agttcaagta ggtatatcag
      841 aaaacaaaat cgatctcaat ggaatcaaac tcaaagatat tcctgtcatt aaaagtatta
      901 cagggagaga gattcatcaa caaccggatg gcagttttgc aatccagatg ccgatgataa
      961 acccattcta ctaccaggat ccaaggcaaa atgtcaaaac agacatgatg ctcattgctc
     1021 aagctataaa agcctccggt tatgactcta ttgaaatgaa ttgtaatttt ccaaatagtg
     1081 aaaaggtagc gatggaaagg ggcaggcaag cttttgccgc ctgtattgaa gcgggatttc
     1141 ctgttgacaa gatcaccatt aatgttaacg gcaaacctat gaaggcggac gaattgttta
     1201 aagaacatcc gaaaacatac caggatatta tgtcaagagc ctcaaaaatc tcgaaagatt
     1261 acgaggacat caaagcgccc aaaacatcaa ctgccccagt gaacactgtc gtcgtcaaac
     1321 aggaaatcgc cgcattacgc gccaaacagc agcaagaaga acgagctcaa acacaacctg
     1381 ttgatgaaca acgacatggt atgaccccca ataattaact tataagttga atttggattg
     1441 tttaatccaa attcaacaaa ttcattatct tccctgaaat ttgctcaaaa aatttcacta
     1501 cctcaaacca tcaatttctg atattttatc cattctagac actttatttt taaatgacag
     1561 attctctgta ccgcaactta ttagaagtat ccataaagcg aaaaataaag tgccagtata
     1621 actattccag gtattttggt atcatcgaga atagatatgg aattcaacgg gttagctaag
     1681 gagaaagcta tgagtactcg tcgctttgat gaatttgaca atgaaatgga tgattttagt
     1741 gatgctgaac ctgttataac tgaggcaagc ccttcaacaa aattggaaag acgtagaaaa
     1801 atagaagatc tgttggagga aaagcggtta agagaagaac tggaagagtt tggttagttg
     1861 gaagaaaatt gaggcatata aatgtcaaac cttacagtct taccatcaat tgaggcatct
     1921 ggcgggagta acggttttac cttttgtggc cacttgacta caactcttga cttaacatga
     1981 gataaagaca gctcgattaa ctccattgca tcatgatccg ttccaattaa ttgctgcaaa
     2041 acttgcatct ctttcttaac caaagcagat ttgtttcgtt ctgggtgcat gggatcaata
     2101 taaataatat cgggataatc cttttcttgc aaactatgca ggaatgaaat agcgtcactt
     2161 gcaatcaaag acaaacacat tttttgaatg tcagcttcgt ttctacgaga tagtgcatcg
     2221 gtaagcagtg cggccatgac cggatggcgt tccagcataa gcacatcggc accaaaagtg
     2281 gccaaaatag cagcatcttt tccccaacct gccgtagcgt ccaaaatttt aatgcctttt
     2341 gttggcttgc aagccctgat caaaccctgt cttttaccct ctcctcttct tttactccag
     2401 gtcatagcac tgaaatcagc aaacataaga gaaaagtttc gaatttttaa agttaatttt
     2461 tcagaagtaa caaaaaggca ggtatcagca ttttgatcta actgtaaatt caattgatca
     2521 gccaacaatt gtgctttttc tttgagttca ttattttcat aaccaactgc ttttattcta
     2581 ttcattctta caataataat tgattgacat actgtaacag ggaattataa attggtactt
     2641 taggatattg tgccaactca gacatatcag tcatattaga aagtacaata ataggctggg
     2701 caccaactgc caatgctgct tgtatatctg aaactctatc tccgacaaag ggcacgctat
     2761 ccagtgaaca atttaacttt tctgccagag cctttagcat tcctggctgg ggttttcggc
     2821 aaaaacagtt gtcttcaggt aaatggatac aatactctag ggcgtcaatt tgcccccctg
     2881 cttctgcaag cacctctctc aacttacaat gaatatcaga taaatcatcc tcagagtata
     2941 gccctcttga aactccagat tgattggttg caacaccgat tttatatccg gcagcacata
     3001 aacgagctat cgcagtaatg ctatcgggta taataataag ctcatcagca ttcttaatat
     3061 aattgattga gtcagtgtta attactccat ctctatcaag caatataatt cgattcataa
     3121 gtgctacaac attgaaatgt cagctacacc ctgcaataac tcttgtaatc gctgcaggag
     3181 cgcaagcctg ttctgtttca atgactcctc ttcaaccata accatcacat gatcaaaaaa
     3241 agcgtctact ggctctttaa gactagccag aagcttaagt aatgcttggt aatctccggc
     3301 gatgtataaa gtttctactg ttttagtaat tttattaaga tgatcaaata atgctttttc
     3361 agcgccttcc tctataagct gttccttaat atctactttt ttatccgcat atgctgcctg
     3421 ggccaagata ttgccaacac gcttacaggc agctgataat gatgtcgcct ctggcatagt
     3481 aataaatgac tttaaagcga ataaacgctt atctaaatca tataaccaat catcctgacg
     3541 agcacgaaca gcatgaacca aatcagcgct gataccttga ttttgataat aagattgaag
     3601 tctctcaaga ataaatggtt ttaattcagc cactaaattc ttgtcttggg ggagtcgatc
     3661 accataatta gccaaggctt ctttaatcaa agttgataaa ttaagcggtg ccgatattga
     3721 aatgagtaaa cggactacag ccaaagcgtg gcgtcttaat ttaaaaggat cttttactcc
     3781 agttggcttt tggccaatcg caaaaatacc caccagagta tcgatacgat ccgccaggga
     3841 aagtgccttg cccaaatcag tcgatggtaa atcatcacca gaaaatctag gcatgtattg
     3901 ctcatttaaa gcggtagcaa cagcagcttc ttcgccatca ttaagagcat aataataccc
     3961 catgatccct tgcaattcag gaaattcgcc taccatacct gtcatcaaat cacacttgct
     4021 taataatgta gctcgctcag ccaggtgaga aggtaaattg agagattggc ttaaatgatt
     4081 catcattgcc tttattcgtt gtaccttatc atacatgctt cccagcttta cttgaaaaat
     4141 aacctgctcg gtagaaggta tatgcgcgct tagcggctgt tttttatctt gtttaaagaa
     4201 aaaggcagca tcacttaatc ttgccctcat taccttctcg tttcctaaaa ccacttgttt
     4261 tggattggaa ctggcgatat ttgataccgt aataaaataa tgtagcagct caccatactt
     4321 attttttagt gcaaaacact tctgatgaga ttgcatggaa gcaataagag cttcagcagg
     4381 aacctctaaa aactcctgtt caaaatttgc aatgagcgct tgaggccatt cgactattga
     4441 ggtaacttca tcaaccaatt cttcaggcat aaccactgaa gcacctagac gtgctgctaa
     4501 ttcttgtacc tgatgtataa tggcttgtcg cctagtagca aaatcggcaa ttacaaaagc
     4561 atctttcatc tgatcttcat aacttgcagc tgaatttatt cttatttcct gaggatgatg
     4621 aaaacgatgt cccctgctat gacatccact tgtaacgcct aaaattttat gattgagtgt
     4681 ctcacttcca taaagcatca cggcccaatg gacaggtcgg gcaaattcgt cgtctccttc
     4741 cccccatctc atgggttttg cgataggtaa agactctaaa gacagactaa ccaatccagg
     4801 caagagatct tttgttttag taccctcact ctgagtttca aaaacaatcc attcaccttt
     4861 gtcagtttga atcttggtaa gagcatctac cgtaacacca catgattttg caaatcctaa
     4921 caaagcagga gtcgggttac cttcactatc gtatgctgct attgcagcag gaccgcgtcg
     4981 catgactttt tggctttcct gttcagtttg taagtcatga atcacaatag ccaggcgtct
     5041 tggtgtagcg aatctcttga cttcaccata acttaattgc gccttatcta atgcggctaa
     5101 caatcgagta gaaaactcat ttgccaaagg ccaaacagcg ccagaaggca gctcttcaca
     5161 tcctaattca aaaataaaat cattgaccat tacacacctt ttgcataggg aaacctaacg
     5221 cctcacgcgc ttgatagtaa gcttgagcta ctgctcttga tagatgtctt acacgtaata
     5281 tatacctttg ccgctctgtc acagaaattg ctttacgtgc atccagtaag ttaaaagtgt
     5341 gagaagcttt tattaccatt tcgtaagcag gaagaggtaa ttgcaaagct atcaattttt
     5401 gtgcctctcc ttcataataa tcaaattgct taaataattc ttcaacattg gcatgttcaa
     5461 aattataagc tgacatctct acttcatttt ggtggaatac atccccatat gtcactatac
     5521 cattagccgt tttacaccaa ggtaaatcaa ataagttatc cacccctaaa acaaacattg
     5581 cgatacgttc caaaccatag gttaattcac cagtgattgg tttacataca agtccgccca
     5641 cttgttgaaa ataagtaaac tgagacacct ccattccatt ttgccagacc tcccaaccta
     5701 agccccatgc gcctagagtt ggtgattccc aattgtcttc gacaaaacgg atatcatcca
     5761 ataagggatc tactccaagt gcgcgtaacg aatccaagta tctgtcttgt atatccaatg
     5821 gcgacggttt taataccacc tgaaattgat aataatgctg aacccggttt ggattttctc
     5881 cataacgacc gtccgtcggc cttctggatg gctgaacata agctgctgac catggctcag
     5941 gtccaatcgc tcttaaaaaa gtagctggat gaaaggtacc cgcgccaact tccagatcca
     6001 agggttgcaa aataacacaa ccaagatctg accaatattt ttgcaaggtc agtataattt
     6061 cctgaaaagt agtaggttta gtcatgaaaa tctcaaaaaa caaatatgat ctttaaatat
     6121 aggttggttt ttaatcctta tatccaggat atatcccata aaacctatct ttcatttaga
     6181 gaattaatta ttcagtccaa taaattattg gagtgttggg taccagagtt gattgagctc
     6241 aacgctacaa cactaaaata ctatcccagt tgagggcaac tgggatatat aaatttaatc
     6301 caaatattta gataaatact taattgccag ccgccttttt aattaactca cgtagtgatt
     6361 gctcgcttgt agcgcctggt ataaatgata tttcactgcc ctttttatat tgaccatctg
     6421 gtgttgagcc aattataaag gctggtgtac ccatcaaatg taatttttca gccagttggc
     6481 gattcgcatc caggatatcg gtcacttcct gactatccat atcttttttc agcttttgca
     6541 tatccaggcc tatggatttt gcggcatcca tcactgtttt ttcatccaaa cgtttgtcta
     6601 tcgtaatcaa agcattatgc atagcctgat atttgccttg catgccagcc gccagagcaa
     6661 ctctggaagc cagatcagaa gtcttgccaa agattggaaa ttctttgtat ataactcgta
     6721 atccgctgtc ttttttaacc agattttcaa tggtagatgc catttttttg caatgaatgc
     6781 attgataatc aaaaaactca accagtgtta cattcccttt aggattacca acagtagtta
     6841 atttgccttg aaaaacttgt tcagcatttt cctgaattgc ggcttgagct tgctgttgca
     6901 tattttgttg ctgtttttgt tgcaatgctt gcgaagcctc caataaaact tctgggttat
     6961 taatcagata atcatgaatg actttttcaa tttccttttt ttgagcatct gataaagaag
     7021 cgctagccgt atcagccgcc attattgcag gtgatactaa tgtagaggcc aatgctccag
     7081 ctgttaataa acttgtaaat ttcacgtaat tccccttact tataattaat caatgacgca
     7141 aattacatct cgcagataac tttatcatta attgatctgc tcaaaacagc aaagtataac
     7201 aaatttctgt tgcttaacgc accctagcat tcatgaaaaa aaaattcaat attacaacaa
     7261 caaactgcaa gtcagggtgc ctcaggtatt ttataaaaat taaatgcaat aactacaggc
     7321 attcagtcaa attagcatag gccaaaacca accattttac accatgttca ctgaatttta
     7381 cttgaactct tgtatgagcc ccgctgcctt caatggcaag aaccacaccc tgtccaaatt
     7441 tggcatgctg cacattttgc ccaattgtaa tgcccgacac ctcgtcagcc actggcaatt
     7501 tgctttttgt agttccaggc cactgagaac gcgatttaac tcgcacttca tcaagaaatt
     7561 gttgaggaat ttcacgcaaa aatcttgatg atctgtgata ctcttcacga ccatattgtc
     7621 ttctgacctc agcataggat aaaactaatt tacgcatggc acgagtcatg ccgacataac
     7681 ataatcgacg ctcttcctcc aagcgaccag gctcttcaat cgattgtctg cctggaaata
     7741 ttccttcttc catgccaacc aaaaacacaa tcggaaactc aagccctttg gctgcatgta
     7801 aggtcatcaa gtgaacggag cgttcatgtt catcagcctg caattccccc gattctaatg
     7861 aagcatgagc aaggaatgaa ttgaccaacg gtaacttctc ctcttcatca tactcatatc
     7921 gaaattgttt ggcagcatta attaattctt gcaggttatc cacacgtgat tctgatttat
     7981 cacctttgat tttgctaaag tgggcgtata aaccactatg ctgtattaca tcagagatct
     8041 gctcatccaa ctccttatta gcaaccacaa cctgtaattt ttcgatcaaa tcaataaact
     8101 tggctaatgc caagctcccc ctttgcccta atcctgtgga ttgaagtatg cccttggaag
     8161 cgccccacaa cgaacactgt tctgctctgg cgtactggcg aacctcatcc aatgtttttt
     8221 cccctattcc tcgagtagga aaattaacca ctctttcaaa cgcagtatcg tcatttggat
     8281 taactaacaa tcgtaagtaa gccaaagtat ctttaatttc tgccctgtca aagaaacgga
     8341 ctcctccata tatcctgtag gctatacctg cacgtaacaa agcctcttcc aaaacacggg
     8401 attgggcatt cgaacgatac aacactgcaa tctcatccgc actggcgccc tgattcaatt
     8461 ccatgccaat tcgttcagta acaaaacgtg cctcatctaa ttcattgaat gcactgtata
     8521 caagaatttt ttctccctca ctaccagcag tccataactc tttacccata cgagtactgt
     8581 tgttgtgaat caatgcattt gctgcattta atatcattgc agttgacctg taattttgtt
     8641 caagtcttat gatttgggta tccttaaaat catgaataaa ttgttgaata ttttccactt
     8701 tggctccacg ccaaccataa attgactgat catcatctcc tacagccaat accgctgtat
     8761 gatcaccagc aagcaaacgg atccatgcat attgaatcgt attggtatcc tgaaattcat
     8821 caactaaaat agcctggaag cgttcgcggt aatgagcaag aatctcttca ttatctcgta
     8881 gcaattcatg agttcgtaat aatagttcag cgaaatcgat tactcctgag gtttggcaaa
     8941 cgtcttcata cgctttataa attgaaacca aagtcttggt cggaccataa tgcagtgcat
     9001 taatatgctg tgggcgcaag ccttcatcct ttttactatt aataaatgct tgcgcttgtt
     9061 tgacttgcca ttgctcagga tcaagattaa gtgaaagaat gactcgcttg ataactcgcg
     9121 cctggtcttc cgaatccaga atatgaaact gttctggaag gttagcctcc ttgtagtggc
     9181 ggcgtaataa acgatggcat aatccatgaa aagtaccaac ccataaaccc aacgtcggtg
     9241 tagacaacat actgctaagt ctcgatctca tttcaccagc cgctttattc gtaaatgtta
     9301 ctgctaatat agcgtgcggg gagagatgct gttcttcaat taaccaggca attcggctca
     9361 ccaatacttt tgttttacca ctgcctgcac ctgctaaaac cagcatatta cctaatggag
     9421 aggttaccgc atcacgttgc ttttcgttta atcccttgag aagtgctgct atggtcatag
     9481 aatatttcca acagaaaaag tgcaagtatt atcttcaatt taattgctaa agcaaagata
     9541 taatcgatgc ggtaaaaaat ctaaaaattc tcctggatat atattgataa aacccataca
     9601 ggtattgctt tctgatcaat cagagcgcaa aatactgttc ggttaaagta gctactagat
     9661 actattataa tacaatgacg ttatcataaa ggactaaaat gcataccctc tacccagcaa
     9721 ttaaacctta cacgcaacac gaattgaagg ttagtaaact gcatacactt tatttggaag
     9781 aaaccggaaa tcctgaaggc atacctgtta ttgtattgca ccatggccca ggtggagaag
     9841 ctgacccgca tctaaggcgt ttttttgatc ctcaacgata tcgcattatt ctttttgatc
     9901 agcgaggttg tggccgttca attccacatt tggaaataag tgaaaataat acccagtttc
     9961 tcatggagga tatcgactcc attagagatt tcctgggctt aagggaattt gtgttatttg
    10021 ggggtggatg gggttctctg ttagcattat tgtatgcgca aaaatttcct caacaggtga
    10081 aagcattact tctccaccaa atttttttag gtcgacaaaa agacatcgat tggttttata
    10141 agcatggagc cagtctggtt tatcctgatt actgggaaga gtttaccaac acagtccccg
    10201 aaaacaaact tgataatatc cccaaatatt atgcggaatg tctacaaggc cctaatgagc
    10261 tagcgcgaat ggctgctgct aaaaactggg ctctttggca agccagatgc agcagtttac
    10321 agccacactt atatgttatt gatcagtata gcgacccaca tttcgcatta gcattagcaa
    10381 ctctggaatc ctattacatt attaatcatt attttattga agaaaatcag gttatagcta
    10441 atgcccataa aataaggcac attcctacct atattgttca tggccgtttt gacctggtaa
    10501 gccctttatc cagcgcatgg gaacttcatc aagcgatccc cgcttccaat ctaagaattg
    10561 tcagagatgc cggtcactct gatagagaat cgggaattat tgatgcactt atttatgcca
    10621 gtaaggacat ctcaaagcag ggtcttgatg catgttaact gtattacaaa gagtaaagga
    10681 agccagggtt gatattgatg gtcaaacagt tggcaaaata aaccatggct tgctgatact
    10741 ttgtggtttt gaacctaatg attcgctaga aaatattaaa cgaatgctgg ataaatgcat
    10801 taattatcgc atttttgaag acccgagtgg caaaatgaac ttgagcttaa aggatgtaaa
    10861 tggcggtttg ctattagtac cacaatttac tctcatggca gatacccaaa aaggattacg
    10921 tccaagtttt tcaaatgcag cttctccaga acttgggcgt gaactttttg ataacttatt
    10981 aacccttgct cagaaatgcc accaaaatac tcaaagtggt tgttttggag cgaacatgca
    11041 agtttattta tgtaatgatg ggcctgtgac atttctgtta caattttaat cagcttttgc
    11101 tgcattaaat ttttgtataa cctggattgt cgagcgaagt ataatcaata ctcatcattg
    11161 ggattcattg cgaccaaaaa aaagcatgtt tgtttcagtt aaaaagcagc aatttaaaag
    11221 gtctattctt tacatcaaag catttgtttg caagaaccaa aaactggtgt aacatcattt
    11281 taggtattta ataaatagaa taattatgcg actaatctta atgtttgcta gcatcatggg
    11341 atccgttatt ttggcgagct gtgcaagtaa aggatcaaac ccgatcgatc catttgaacc
    11401 ctttaatcgt aaagtgcaca attttaatat ggcatttgat gctaccgtat taagaccacc
    11461 agcaaaatta tatcaggctg tcgtaccaaa attggtaaga aaaggtgtga ataatgcatt
    11521 caataatctg gatttgttcc ctacaatagc cagcgatata ctgcaagctg aaggaaaatg
    11581 ggtaatcaaa gacggttggc gactcttcat taactccact attggggttg ctggtctgtt
    11641 tgatgtagca gaaaaatttg gtttaccacc gcattacaat gatctgggcc taacactcgc
    11701 caaatggggc gataaaaaat ctccttacct tgtaataccc tttttaggcc ccagtactct
    11761 tcgtgatgga gccggctggc tgttccagtt tgcactctgg agtccttatg tgtacattga
    11821 tgatgctggt ctggtttatg gcttaattgg attgcgctac attgatttac gaacacaatt
    11881 atttgactct gagcctttgc tgaatgaagc gttagacaaa tacacattca tccgagacgc
    11941 ttacctgcaa catagaaatt atcttattgc agggactgag caagatacag gcgcgtcctt
    12001 cgttgaggat gcgaaagcta acaaaaccgc aatgcaagaa tcaagcgctt caaataatca
    12061 actaggttct gactatgttg atgaataaac tgtttaattg agacttgcca ggtcataata
    12121 agggttggcg ttaagccaaa tgcaaagcca actctcttta acctctgaac ttcaaaatca
    12181 aaataatgac tacaattctt tattttgata aagaagcatg taccttttca aggcagcgca
    12241 tatattcacc tgcgatatcc aaaccagttt cagtgctaat ttcccgaatg caagtgggac
    12301 tggtcacatt aatctctgtt agataatcac ctatcacgtc gatacctaca aaatatagcc
    12361 ctttttctct caaaactggg gctatttgtt cacatatcca tttgtctctt tcagtaatag
    12421 caaccacttc tcctctggct cctgcggcca aatttccccg caaatctcct tttgcaggaa
    12481 tgcgagccaa agcaaaagga acggggtctc cattgatcaa taaaatacgt ttgtctccgc
    12541 ttactgtaat atccgggata taacgctgag ccataataca ttttgtctgc cctttggtta
    12601 atacctccaa aatgaccgat aaatttttac cctctttgtc aacataaaaa acggaattac
    12661 ctcccattcc ttcaagaggt ttaaaaataa cttgttgatg aatttgccag aactctttta
    12721 attgccgagc atctctggat acaaaagtag gaggacagca atgaggaaaa tttagggtaa
    12781 aaaatttctc attcgcatca cgtaaacttt gcggtttatt tgcaaccaat accccttctt
    12841 gttccgctaa atccaaagcg taagttgcat aaatatactc catatcaaac ggaggatcct
    12901 tacgcatcaa aataacatca aaatctctca atggcttatt acccaaatct atggttttaa
    12961 cccaactcaa actctctata tctccaatgt gaatttcatg gactcgtgca taagcgctcc
    13021 catcacaaca atataagtca ttctgggtga agtaggcgca agaccatccc aatccctgtg
    13081 cgcttttaat catagctact gtagagtctt tatagggttt tatttgatcc aatggatcca
    13141 taagcaccgc aagtttcatc attcgcctcc aattgcagca atttctcgag ctgcagccag
    13201 tgcagccaag cgagctataa ccccataaac ataaaaacga tttgggcagt caactacctc
    13261 taaatcatca catggcatat tacaagcctg tgcgaaagct aacggctcaa aatgcatgcc
    13321 tggagcattg aggttctcat caattcctct gcctttatga acgcgataaa atccaccaac
    13381 aacaaattga ccaatcatat aaactacagg ttcagcaaca gaaccatcag gcatcgtttc
    13441 aaaagtataa acgccttcct gaataatgac tctgtctacc ttcctgcttc ctttactggt
    13501 agacatcctg gtacgttgct ttctattcaa ttgtcgaatt tcatctccat tatgaaccat
    13561 cataacgctc atgccataag taccattatc tgcctttaca actgcgaacg gtttattttc
    13621 tattccgtaa gtagtgtatt tatcgtttat taacgataat atcttatcga cttcttgagc
    13681 caaggtttca atcccttcct gggccataaa atcgaccccc tctatagcgc tgaaataggg
    13741 attaattaac cacggatcaa tgccgactaa ttcagcaaac tcaatagcta cttcttcaaa
    13801 aaattgaaaa tgactcgatt ttaatcgaga agcccatcct aatttggctg ttggcctaat
    13861 tcgctgttgc acatcctgta atatttcagg aactcctgaa gacaaatcgt tattcaataa
    13921 caaaaggcaa gggctgaaat tatccagtcc taccttatca ccttgtcttt gtaatggttc
    13981 aattaataaa gtttcacctt gttcgagtat tatttctgta ggttctttaa tctctggatc
    14041 taaactgcct attcgaacta taaaaccggc ttttacaaaa attgttttta atacattcaa
    14101 actctgtaaa taatacttat tccgagtatg gctttctgga agaagcaaaa ttttagtaca
    14161 atcagggata tattctatca aggcagattg agcggcttgt atacacaggg gaagaaactc
    14221 cgggttcaaa ttattaaaac cggctggaaa taaattggta tcaacgggag ccaatttaaa
    14281 gccagcatgt cgcaaatcaa cagatgacgt tagaggtgcc ggcgtttcct gccacttttt
    14341 tctgaaccag gattctattt cagctagttt atttaaaatt attttttcca catgatgcaa
    14401 aggacctgaa tgagcagtag tcagatgtgg aacggggatt tcatgcttat tcattcatac
    14461 tccctatttt ttaaaatgga tcgatgttac ctaggttatt cataaaataa aagtatttat
    14521 cattcttaat acattattac tacagttttc atgatgacaa catatataga aaagagaggc
    14581 atttatataa tagtcgtgtt tcacaacaac atattgtgaa tacagcatta tttattccat
    14641 catcgattct tcatattctt gaccaggatt tttggcaaaa tgtactgcaa tcatataaaa
    14701 taataattca gcatttgcag ggattaaaaa aactgtttta ttgagcaaaa tttaaaagcc
    14761 tctcaaggat atcgttattt tccactttca atggcataat tatccaaaat tatactattt
    14821 atcctacctt ttatgctcca tttaaaaacc atacaatcaa tccttcttct aattgtttta
    14881 ggattcatct ggggatcggg ttatactctt gccaaatttg caatgactaa tggagttaac
    14941 ccactaggct acgctttttg gcaatcatta ggaccagctc tactattaac tttagtgagc
    15001 ctctttacaa aacaagctcg cttgctacac cctgtttatt ggccttattt ttttatatgt
    15061 ggattgatag gtatcgctat tcctaatacc aacatgtatt ttattgccag ccatatcccc
    15121 gcaggtttat tggcagtgtt agttaatact gttcctttac tggtttaccc tttagctctc
    15181 atagcaggcc aggaaaaacc ggatggctgg cgattttttg ccttgttagt tggtatgtcc
    15241 ggcatacttg caattatagg aataaactcc gcaggacttt actcaagctg gactatatta
    15301 gctatgctta gccctcttgg ctttgctttt tgctcaattt atataggtgc caaacagcca
    15361 aaagaaatta acgccctaca agctgctaat ggaatgttac tggcggcgtc tcttctactt
    15421 acccctgttg tcctcaagca gcacgcattt tattctctct cagctccgtt aactctcgtg
    15481 aaacaaatcg ttatccttga aataatttta tccagtttag gatatttgct tttctttatt
    15541 ctcattcgta tggcaggacc tgttttttat agtttaactg gtggagtcgt tgcaataacc
    15601 gggttgtttt gggggtactt ggcttttgat gagaagcctt catctataca gagcatggca
    15661 atcgctttag tggtttccgc catttttctg ttatcatgga gacaatcgaa acaaactcaa
    15721 ggaatctgat tcatgctcag tgaattagca aaacaattta gcacacaaat ccaaaccttt
    15781 ttctatttaa ttatgttaat taatggtctt ttacatttta tctttgccgg agcagtggct
    15841 cgagacgcag gtaatctcta taggctaggt caaaaacctg ttttagtatc ggcccctacc
    15901 tgggcatttg cgactctaat tggaggagtc atcacagcaa ctatatactg gctgcttcat
    15961 cactccacat taaccagacc cactatgaga gaaattcgtt atgacaaagc ataagatcaa
    16021 aacaatcgac gttcatgaac taaaacacca aatggacaat caatccaatt tgagtttgat
    16081 tgatgtacgt gaactggagg aatgggaaat gatgcatatc ccgggagcac ttcatattcc
    16141 taaagatcgc atctctactg aaatacaaaa ccaaataccc aataaagaac aaactatcta
    16201 tttacattgc cgaagcggtg tcagatcact gtatgcagct caatgcttaa tggatttggg
    16261 atattacgaa gtctattctg tggatggtgg aattatggct tgggcaatga gtggttatcc
    16321 ggtaaaacag gagtcttaca ccccttcctg agtgaagagg tgagaataaa agagaatgag
    16381 ttgcttaaag gcgatgagtc agggcttcct gagtttcagt gactttttct tttcctgatg
    16441 gaacactaaa aagaccgcct tgagagcttt ttggtttttg ttgcagtaaa tgcaaatcgc
    16501 gagcggcgat aataatctcg cgtcttaatt tatgcactct atcagataac tcaagaatat
    16561 catcaacaac agaataatgc ttgattgcga aaggttgttt atccaaatac ccttttcttg
    16621 ggtcagaatt tttataaaca tgagaacgca ggaatttttc caaagcaccg tacatatcag
    16681 ccaaatcctc ccgattagga ttattatcct tattcaagtc aagcgatatg gtaagagatt
    16741 tataaaaatc gctgttgtcg ggagaagtgt atttataagt tgcagcaatc tgatcacgaa
    16801 tgtaataggc caatgcattt aatacgcgtg ccttatcttc tggttctttg gatgaatgat
    16861 ccagcagttc gatagttttt agcaagacaa gtacttgtgg ttgcctttct cttgaaactt
    16921 cttttactga gtcctctaat ttgtcctcaa taattttggc tttctcatta tgggacttga
    16981 ttaaagtaat gatactttcc ctgatgcttc cttttaattt ttcatatgcg accaattcaa
    17041 atgacatctt attttctcct tatactccat gaacaggact agtgaaatgc tttcactgta
    17101 ggccagttta aaccaattcc ctcttttcgt catgtttttt ctttttacta tctaattaat
    17161 tttcaatcta ttgattttaa tgaaaaattt gcaatttcct tgttagttca tcaaatgaat
    17221 agacttctgg agtgcattgt taatgcattg ttatcgttga tttaagagta agctcagatc
    17281 aattaaatca agatattttt tgctcaatca gtcaggcaca ataaaaatta gaagaaattt
    17341 aaataagcta tttattgttt ttataaaaca tttgatttaa tttacgcgtc aatttgcaat
    17401 atatgatggt cataaaaatg aaaaaaataa gctgcagtat tcttcttttg atcaactcct
    17461 taattagcta cgctgattca gaccaaccta aattaattct acaaatagtt gttgaccaat
    17521 tacgcggaga tctgatttac cgtcatcata aacaatttgg accttctggt ttcaattatt
    17581 tgcttgctca tggtttggat tatcataacg cacatcatcc acatgccaat acaacaacct
    17641 gcgcaggcca tagcactatt gcgacaggaa gctacccctc tttacacggc atagttgata
    17701 atgattggta tgatagaaaa acaagaaaac taaagtactg tatggaggat ttacaagcca
    17761 aaattttacc cacagtacac acccatatcg aaatttcagg acgctcaccg cgaaacctaa
    17821 aaccatccac attaagcgat gaaatcattt tagcaaataa agggaaagcc tttggtgtat
    17881 cattaaaaga cagagcagct atcactctag ctggtcacgc aggaaaagcg ttttggtttg
    17941 acaaaacgaa tggtggattt attacaagca attactacta ttacagttat ccactatggg
    18001 tagaaaactg gaataaactc tatcaaccca aagcctacga ctggaatctt tctcatccta
    18061 aatcattcta tcaaaatgca aatactcttc catttcgtca taactatggc agctttggtc
    18121 aaacatttcc ccatcatgta accaatcctc catctgaaga atatttcaaa tcgctttcca
    18181 gaacaccaaa ggcagatgaa ttaacagctg attttgccaa acaactactg gttaatgaaa
    18241 aattggggaa aacaacaggc caaacagatt atcttgcaat cagtttttct gctgtagatg
    18301 ctattggtca ccaatttggg cctaatagtc tggaagcaga agacaatttg atcgcccttg
    18361 atgacaccct aagccaactt tttaaaacca ttgataaaga ggttggtctt aataatactt
    18421 taattatact caccgcagat catggcgtca gcgacggtcc ttcttacctt aaagcaaata
    18481 aaatacctga aattaaacct gttaatatag aagcaacagg aaaacacatc gagaatttat
    18541 tattgaaacg tttcaacctg cccacgcaag ccttaatgtc aattaatcct ccttttatct
    18601 accttgatca ccaaattata aaagagaaaa atttggatat tgcagaggtc agtttctacc
    18661 tggcagaaga aatcagtcat cttccaggga tattcaaagc ctacccctta cctattcgtg
    18721 atattgaaaa agattggtta agcgcaaaag ttgaccgcat gtcttacgct taccgtgcgg
    18781 gcgatgttta cattgttcaa ccgccatatc aaagccacgg tgcaaaaagt gaagatcgag
    18841 ttgctcatgg cagcccctgg caatatgata cttatgtccc gctactgttt gtacatcctg
    18901 acttcaaacc aaaacgtata ttcaggcaag tacacacaac ggatattgca ccaacattat
    18961 cttccttatt attgattaaa cctcctgccg cagcagtggg acagccttta gttgaagtag
    19021 tcaatgcttt tcataagact ggagagaatg attgaattcc aactgaaaaa actttactat
    19081 tcgtacaaaa tttttaatga aaacatcatg acaaagcaag caattattat tgggatttcc
    19141 ggcccatcag catctggaaa aagtttatta gcaaatacta tagtcaatga attaggatcc
    19201 gagcaggttg tcgttatctc tgaagatgcc tactataaag ataatgggca tctacccttt
    19261 gcagagagag aaaaaataaa ttacgatcat cctgactcct ttgatcatgc tctactttat
    19321 gagcatcttc gtcagctacg agttggaaat acagtacaaa ttcctattta ttctcattca
    19381 aaacatctgc gtttacctga aacgagagca gtgggtcaac atgccattat tgtactcgaa
    19441 ggaatactgc ttttcagtga taaagctctg cgcgaaatta tggatatacg tatattcatg
    19501 tcgactcctc ttgacgtctg tttaacgcgg cgcttaaaaa gagatgtagt agaaaggcat
    19561 cgatcattcg aatcggtcat tcatcaatat gagactacag tgcgccctat gtacatgcag
    19621 tttattgagc cttcaagccg ctacgctgat ataatagtac ctcgcggtgg tgaaaacaga
    19681 atcgctatag agatgattca agctaaaatg cgggaattat tagcttcaca tactcgaggc
    19741 agttgatgtt ttattaggct gtttaaaagc aacctaattg atattgtggt tttagtccag
    19801 gatcgaagta acactcactt cgccctgcta aattaagaat aatgggagga gatactattg
    19861 tgggattttt aactggaaaa aaagcactta ttgttggatt ggcaagcaac agatcgattg
    19921 catatgggat tgccaaagca ttccataatc aaggcgctga gttggctttt acttaccaaa
    19981 acgaaaaatt acagtcgcgt gttgaagcaa tggcatcgga atttaattca actctagttt
    20041 ttccttgtga cgttgcaagt gatgaagaaa taaaagccgt gtttgataat ttgcgtaatc
    20101 actgggataa attagatatc ctggttcact ctgtagctta tgctcctgct gatcagatca
    20161 gtggtgattt tgtggaatgc gcaaatagag aaggatttag aatcgctcat gatattagtg
    20221 cctatagcct cataggtttg tctcaagccg cattgcctat gatgcttgat actcagggtt
    20281 caattttaac cttaagctat tatggggcag aaaaagccgt accaaactat aacgttatgg
    20341 gtgttgcgaa ggcaagtttg gaagcctctg tgcgctatct ggcagcaagc ttaggatcca
    20401 gaggattaag aattaatgcc atatcagctg gaccaattaa aactcttgcc gctgctggaa
    20461 ttaaagattt tcgtaaaatt catgcagcct atgcaaacat tactccatta caaagaaatg
    20521 ttactgctga tgaagtagga aatactgccg ccttcttatg ctctgatctg gcatctggga
    20581 ttactggtga agtgctacat gttgatgcag gttatcacgc agtatcagcc atgagcgaac
    20641 taggctagat aaatattagt ccgctttaaa ttgctgactt aattcttagt tgtacttaaa
    20701 aaaattcaat aaagctaaat tatattgggg ctgagcaaat agcgaggccc aagctaccaa
    20761 acgtttttta taatataaat ttagcttatc taagctattg aatcgacatg aaatagatca
    20821 cctcaatcaa atgaattcct gcgactcctt tattaacgga ttattattaa gagaacaaac
    20881 ctcaacctaa aaagacttta tttaactgat tttgaataaa aataatcctt caaaagctca
    20941 tgcaactatc cgcgctctat tcaggatcat tttcgtccag cctgaacccc aaactagccg
    21001 ctataaaata tttcaacgtg taagtctctg aactgtttca ggcgtattaa taatatggat
    21061 gatacttttt tatcagaata aactctgaaa tagcctcact gagagaagct ataggtaaaa
    21121 ctgaagaatt cttgatatct ggccatccct taatttcatg aggaacagcc attttcttgt
    21181 cctatttcct ttctattttc gcatgatgaa gtaagttatt aacatataaa tcataatcca
    21241 tcattccata gctagcttct atttgctgta tcaagctatc tctttgctct tgatccaaag
    21301 agcttaattt tccatcatta attcgtttta atttaacaac aacataatcg ccgttgtcta
    21361 aaattactcc atctcggctt tctggacgta ataaattaaa tgctaaatca ttaattaaag
    21421 aatttgcttt atcactatcc ctggaagcat tctctacaga atgccattcg aattgattgt
    21481 tagcgattaa ttcctgctgt tgcttatctt caacggggtg aagcaaacta ctaccaattt
    21541 cttttgcttt agcatcccca tactgttttg caagtatttt tttaatctga tctctcactt
    21601 catccaaggt ttgttctctt gaaggtatat gtttcttcac tcgtaaaact attacagagt
    21661 cattatcaat ctgtataggc tcgctattat tccctagttc taaaacatca gtgctgaatg
    21721 ctgcatggat aacctgctta tttttggtaa taggttgctt gccaccatca cgagaaaaag
    21781 gctctgtatg ttgaattttc agctttaatg aatccaaaac cggatcaagt gaatcaggag
    21841 tttgataact taaatctgtt aattgctcta aaacttgagc gtattttgcc tgagccatat
    21901 cactggttag ttgatctttt ataatgtttt caacttcttg caaagattta gttgtaactg
    21961 gcttgtaagc aatcagctta aaaatttcat aaccatattt tgtttgtact ggtatagaaa
    22021 tttgccctgg tctagttaaa ttggaaagtg ctctgtcata ttcattttgc ccgccagtaa
    22081 tccagggaag aatgccctta ttggctatag ataacttgtc atcagatttg gaagcaacat
    22141 atttatcaaa ttgttctgga tgctttttta aatcgctata aacctcatcg gctttttgct
    22201 taattgaatc ttgttcttcc ttggtggcat tttctgggac tgcaaataaa atatgggcaa
    22261 cccgccattg agcaggagtt aggtagttgc ttttgttttc ttcataatag cttttaattt
    22321 cgtcgggtga tatctttatt tgtgacttga tatcatgcat agacaaaagc acataatcca
    22381 atgtaacttg ctctgaagtc ataaattttt tatggtgttg gttataataa ctcttgatat
    22441 catcttctga aatcttcgcc tgttgttcaa agcgatcaga aggtaccgtc aaataatcat
    22501 aatccctagt ctgcatatac aatctgacaa aacgctttat ttcatctgat aaagcaaatg
    22561 aagttcccat aaacgcaaaa cgttgttgat taagcaacat gccttgcctt acttctttct
    22621 ggaaagtctc tggagtaaac aaagctccac tcaaagcttg ttgatatcgt tgtgccgaaa
    22681 aatgcccatc ctcctggaat tgaggaattt gcactatggc cgcattggct tgttctgggc
    22741 taacctcaaa tccatatttc ctggcagcct gaatactcac ctcattcgtt atcatttgat
    22801 ttaatacttg attttgcaga tttttttcat cagcggcagt catctgtggt aaatcttgct
    22861 gggcacgagt acgtctatag ttcgtttcaa acgcttgcat ggtaattggt ttatcattaa
    22921 ctattacctt tgcatcggat atttgtcgag attgaaaata ataatcaaca ccaaaaagtg
    22981 taaaggtaat agcaattaat ataataacta accacgctac tacaccctga atgtgttcat
    23041 ttaacttctg caacatgtcc cgagatccat tttgatatta acaatgcttg atgaaaccga
    23101 ttctaccata agctgataaa aaaaacgcgc ctttcagcgc gtttatctgg cggagtggac
    23161 ggggctcgaa cccgcgaccc ccggcgtgac aggccggtat tctgaccaac tgaactacca
    23221 ctccatttct tggtgggtgc tgtagggatc gaacctacga ccctcgcctt gtaagggcga
    23281 tgctctccca gctgagctaa gcaccctgaa aacttaagct tgttgtaccg cttctttcag
    23341 gtttttacct gctttaaatt tggcaacccg tgacgcttta atttggattg tttttccagt
    23401 ctgtgggttt ctgccagtac gtgctgaacg attacctgtt gaaaaagaac caaaaccagg
    23461 tattacaact tgatcgcctt tctttaagac atcagttaca gtagccataa aagtttcaag
    23521 aactctgctt gcgtcagctt tggtcaaacc tgaaccgctt gctatagcat caactaattc
    23581 actcttattc atcttttccc ctttacagtt aatcatccat attccggagc tgatttaaac
    23641 agattcaact gctccagtca agttcgtaca tccttgcaga gcccgccagt agcgggccac
    23701 gaagagaaat atactctgta ttaatgtgcg tgtaaatcat tatttttaat tttttttgat
    23761 cttttattca atatagtgcc aggttgtata tttgttggct cttccaccca tggattgcgt
    23821 tgcaaagcca gttccaaaac ctgttcaatg gtgttaacag gatgaattgt cagcttgcgg
    23881 agcacattat cagggatttc ctcaaggtct tttacattct cctcggggat aatgacatgc
    23941 ttgattccac cacgatgtgc agccagcaat ttttctttta atcctccaat tggtaaaact
    24001 tgcccacgca gagtaatttc acctgtcatg gcgacatctg ccttaactgg gatttgtgtt
    24061 actatggaga ctagtaccgt gcacattcct ataccagcgc ttgggccatc tttgggtgtt
    24121 gcgccttctg gtacatgaac atgaaaatca tgtttctcat aaaaatcatc tggcaaacca
    24181 aaagctttag cgcggcttct tactaccgtc atagctgcat ggatagattc ttgcattacc
    24241 tcaccgagct gaccagtatg agtaacttta cccttacctg gcataatgga tgcttctata
    24301 gtcagaagtt cacctccaac acttgtccag gctaaaccag tgacttgacc tatctgatca
    24361 tattgttcag ctaatccata acgatatttt ttaacgccca aatacttggt aatgttgcta
    24421 acagttatac tgattgtttt tccttttttt ccctttttgc ttgagagtat ttctttaaca
    24481 acttttctgc atatactcgc tatatccctt tcaagattac gtactccagc ctctcgagta
    24541 taataacgta tgatatctct aatagcccct tcgctaatat ggatttcttc attggttaaa
    24601 ccatttagca gcatttgttt gggaaccaaa tatttttcag caatactgac tttttcgtcc
    24661 tcagtatatc caggcaagcg aataacttcc attctatcca ataacggggc tggaatttct
    24721 agcgaattgg cagttgcaat aaacataaca tcactcaagt cataatcaac ttccaagtaa
    24781 tgatcactaa atgtatggtt ctgttcagga tccaatactt ccagcaatgc cgatgccgga
    24841 tcaccgcgaa aatccattgc catcttatct acttcatcca gcatgataag aggattttta
    24901 actcctgcct tgcataattt ttggataatt ttacctggca tggaacctat atatgttctt
    24961 cgatgaccac gaatttctgc ttcatctctt accccaccca gagcgatgcg tatgaaagtt
    25021 cttccagtag cattggcgat agattgtcca agagaagttt tacctactcc cggagggcct
    25081 actagacaca atatcggtcc tttcaatctt tttactcgtt gttgtacagc aagatattcg
    25141 atgatacgct ctttaacctg ctccagacca tgatgctcct tatcgagcag cttttctgct
    25201 tttagtaaat caaactgaat tttggtccgc ttcttccatg ggaccatcag catccaatca
    25261 agataattac gaataacagt tgcttcagca gacatcggag acatcatttt taatttatgt
    25321 aactctgcta atgatttttc tttagcttct ttcggcattc cggccttttt gattgaattt
    25381 tccaattgtt caatctcatt gccttcctca cccatttccc ctaattcttt ctgaatagct
    25441 ttgatttgtt cgtttagata gtattctctc tggctttttt ccatttgacg tttgactcga
    25501 cctcttacac gtttttcaac atgtaacaga tcaatttcat tttctatcgc cgccattaac
    25561 ttctcaagtc gagttcctac atccatagtt tcaagcaact cttgcttatc atctacttta
    25621 agcgttaaat gcgcggcaat ggtatctgcc agtcgacctg gctcttctat tcctgccaat
    25681 ggtgacaaga cttctggtgg aattttctta ttaagcttta tatattgttc aaattgcgac
    25741 atcaaggacc gcattagaat tccaatttct tgctcttgaa ttgttgagcc aacttcctca
    25801 atgtattcaa gtgtcgcttc gaggtaacct ttatcctgag tatactcctt gacacgtgca
    25861 cgctgctcac cttcaacaag aacttttaca gttccgtcag gtaactttaa caattgcaaa
    25921 acactggata cagttcccac ttgatagata tcttctgggc caggatcatc atgcgccgat
    25981 ttacgctgcg caactaaaaa aatttgttta ttatcaatca tcgctgcttc aagagcttta
    26041 attgacttgc cacgcccaac aaagagaggt atcaccatat gcggatagac gactacgtcc
    26101 ctcaatggta atacaggcaa agcagaagat ttcacagtct cgtttgatat tatttcattt
    26161 tcattagaca taatgaccct tattcaaagg aaaatttctt ggttatagtt
//
LOCUS       DBNCWK010000030        25641 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.030, whole genome shotgun sequence.
ACCESSION   DBNCWK010000030 DBNCWK010000000
VERSION     DBNCWK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25641
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.030"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            183..3059
                     /gene="rapA"
                     /locus_tag="AC06IO_001141"
     CDS             183..3059
                     /gene="rapA"
                     /locus_tag="AC06IO_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HIO9874342.1"
                     /translation="MTFRIGQRWISNTESQLGLGIITEVDGRQVSISFPAAGEDRIYA
                     IDNAPLSRIVYKTGEEILTNDQQRIKVTSIQEQDGVLLYTGNDDLGNVAQVSELALNC
                     FIKLNSPQQRLFSGLLDKLNAFKLRIKTLEHLGRLQQSKARGLLGSRTNHLIHQIYIA
                     YEVAQRFAPRVMLADEVGLGKTIEAGMILHYQLHTGRASRVLIIVPNSLIHQWFVEML
                     RRFNLHFSIIGPNQYDAMPSTLDELEDNDNPIEPALEDGNLFEGEQLVLTSLDFLMEN
                     ETARQQALAAQWDLLVVDEAHHLYWSEDSQSPEYTFIEKLSDRSTGLLLLTATPEQVG
                     MKSHFARLRLLDPARFYDFSAFVNEEKQYHEINKIVQDLMDYKEKNGVDELNPQLHPH
                     LKQLLGKEAPAGINATIKELLDRFGTGRVLFRNTRAAIKGFPERRIHPVALTCPALYS
                     EIIRESNLIHLYPETLLNNDSWIDHDPRVQWLVNKINELRPEKILVICAKAKTAISLE
                     QYLKLKTGIRSTLFHEGLTIIERDRAAAYFAEQENGAQVLVCSEIGSEGRNFQFSHHL
                     VLFDLPLNPDLLEQRIGRLDRIGQNHPIEIHVPYLAGTAQEKLFRWYHEGINILQQSC
                     SVGFSIFEVFENRLTPVLSNPLQDTSEQSLEKLIADTQLHTQHIKEALNAGRDRLLEL
                     NSCNYTIGKELIEAIEAEENCLELENYMSQVYQEYGIDQEYHSENAEILRPGNHMKTA
                     HFPGLKEDGMTVTYSRSKALVREDMEFLSWEHPMVYDTMEMILESELGNATVTTISIK
                     SIKPGTLFLETFYTINSSAPKYLQLDRFIPSLPIRILMDTTGKNLSGILSYEQLNNLC
                     QPLKRHLGYPVIKQVREEIESILQRTNKIAEDQMKLFIEEAKSMMELTTSQEVNRLES
                     LQKINPAIRDEEIEFFKKKISESNYFINSSTLKLQAIRVVINVS"
     gene            complement(3173..4576)
                     /gene="ankH"
                     /locus_tag="AC06IO_001142"
     CDS             complement(3173..4576)
                     /gene="ankH"
                     /locus_tag="AC06IO_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkH/LegA3"
                     /protein_id="HIO9874343.1"
                     /translation="MSIANDIISHRMPDFDAYLRAGESLDDIDEYGFTPLIECAITRQ
                     IKIAEQLIARKVDINKPDVTGRTPLHWAVDNNDLDMTKLLLTYGADPNAYTRNGLCVL
                     VYPVLRGQDAIKQLLYHHGAKLDFALDFINAKLIGHRFELQGDVDIVNAKGEFIELDY
                     EGFILEFTVAVVKDSLRRFISSYSTRHLRDYFPYIHNIMDAFTDAAELLQYQHHPRLG
                     EQHFKRIAALLKAPMLILPAASRGHALCFVRYHQWWAKIDRGENSLQEGSVNIYRITR
                     PEALTINFIHDFLYKKQNRRYYHQMINQQLGLLPFAQMPISSQISGNCSWANVQAVVP
                     VAYSMQELTSVENFKPDVALSLYDEWVEWDKDRALDECIQRFYLASPERKASIAAILG
                     GILFQACDHANKRHLERAEKILNILILDEYYYVLSSYLEEYCIKRLTRKGNNLLKILD
                     DCGINPNIGVNPVATGL"
     gene            complement(4577..5350)
                     /locus_tag="AC06IO_001143"
     CDS             complement(4577..5350)
                     /locus_tag="AC06IO_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIO9874344.1"
                     /translation="MAKEYYLSDEIEEIVLASTVLSYSRPNLSSKRFNDLKNGKTPWE
                     AELDLNGLNVESARKSLYQFIQTQIKNNKQCILIIHGADNSQDKPPLMKNLVNRWLPQ
                     INEVLAFHSAKPKDGGSAAVYVLLGSVFHVELPTRLPSNRSESTFLVAETKAMERRRR
                     LAEQQGERRFASVKAREHPEEEIKVAPEGELQNNILQHPELNSQRFDGIDPNLNPEPP
                     LNTEARREFDNEKREQDKEKQLRLGNMPKFTTAPTPRGP"
     gene            complement(5499..6521)
                     /locus_tag="AC06IO_001144"
     CDS             complement(5499..6521)
                     /locus_tag="AC06IO_001144"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIO9874345.1"
                     /translation="MSRHLNVAIVGATGAVGETFLTVLEERNFPIKSLYPLASSRSVG
                     KTVTFRDQELDVLDLAEFDFSKVDLALFSAGGAVSKEYAPKAVAAGCVVVDNTSCFRY
                     EDDIPLVVPEVNPHRIADYTKRGIIANPNCSTIQMVVALKPIYDAVGISRINVATYQS
                     VSGTGKKAISELVAQVGDLLNGRPANVQVYPQQIAFNALPHIDQFEDNGYTREEMKMV
                     WETRKIMEDDSIMVNPTAVRVPVIYGHSEAVHLELKKPLTADDARALLAKAPGVTVVD
                     NLSKASYPTAIKNAVGHDDVFVGRIRQDISHPCGLNLWIVADNIRKGAATNAVQIAEI
                     LQREFL"
     gene            complement(6631..7689)
                     /gene="aroC"
                     /locus_tag="AC06IO_001145"
     CDS             complement(6631..7689)
                     /gene="aroC"
                     /locus_tag="AC06IO_001145"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIO9874346.1"
                     /translation="MSGNTFGALFTVTTFGESHGPAIGCVVDGCPPGMSLTEADIQPF
                     LDKRKPGQSKYTTQRREEDKVQILSGVFDGKTTGAPIALLIQNTDQRSRDYEDIKNLF
                     RPGHADFTYHYKYGHRDYRGGGRSSARETAARVAAGAIARLYLKRYLNLDIIGYLQQM
                     GDLKLRFENENEINKNPFFCPNNKQIQELADYVDRLRRQGDSVGARVKILARGVPTGL
                     GDPVFDKLDATLAYAMMSINAVKGVEIGAGFNAVEQLGSHHRDQMTAKGFLSNHAGGI
                     LGGIATGQPIEVSIALKPTSSITTPGQTINTEGEEVTVVTKGRHDPCVGIRAVPIAEA
                     MMALVLMDHYLRHKAQCK"
     gene            complement(7701..8633)
                     /gene="prmB"
                     /locus_tag="AC06IO_001146"
     CDS             complement(7701..8633)
                     /gene="prmB"
                     /locus_tag="AC06IO_001146"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIO9874347.1"
                     /translation="MVMVDTLVSSYFSKETEELVTILDFLRFSLSCAENAQLFYGHGT
                     DNAWDDMRSLILRSLSLPYDVDPFLLNARLTTSERKHLCNQLDKRINQRVPVPYLIKE
                     AYFCDLPFYVDERVLIPRSPIGELINNQFSPWIEAEQVHHVLDLCTGSGCIAIACCYA
                     FPEAQVDAVDISNQALAVATINCERHDVGDQLALIESDCFTALSGKQYDLIVSNPPYV
                     GKEEMQTLPDEYRHEPVLALETGNNGLAIIEKILKNAHAYLSEHGILVVEVGNSEEAL
                     CETYPMVPFTWLEMSNGGQGVFLLTKQQLEDYFK"
     gene            8799..9368
                     /locus_tag="AC06IO_001147"
     CDS             8799..9368
                     /locus_tag="AC06IO_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIO9874348.1"
                     /translation="MTDDFLSDEDKALFRHHMRSVKPLNEKTKRVKNPTQPTHEKKTT
                     TFKSQPVPKEEYFLSDFIRDTVQPDTILSFSEPGIPSQRFRALKNGQIPWEAKLDLHG
                     LKAEAARQALYDFIQTQSLNNKRCLLIIHGKGGHQGAPPVIKNLINRWLPQFSQVLAF
                     HSALPKDGGLGAVYVLLKRNREKDEQDNY"
     gene            9441..9860
                     /locus_tag="AC06IO_001148"
     CDS             9441..9860
                     /locus_tag="AC06IO_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIO9874349.1"
                     /translation="MEKLGQFIINHWQLWLAFVVVVFLIFINEILSQRKKAKEVSPQS
                     LVDLMNNGNAIVIDIRDKESFKNGHIINSMNVNPDDFEQQKMSKYKDKPIILVCARGL
                     QSRTLAMKIRNQGYQPLVLSGGIQAWQNADLPLVKGK"
     gene            9865..10119
                     /gene="grxC"
                     /locus_tag="AC06IO_001149"
     CDS             9865..10119
                     /gene="grxC"
                     /locus_tag="AC06IO_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HIO9874350.1"
                     /translation="MNEVILYTTGYCPYCIKAKELLDRKKVIYTEIRVDLQPELREEM
                     IQKSGRRTVPQIFINGQAIGGCDDLYALEAQGTLNELLKK"
     gene            10129..10617
                     /gene="secB"
                     /locus_tag="AC06IO_001150"
     CDS             10129..10617
                     /gene="secB"
                     /locus_tag="AC06IO_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIO9874351.1"
                     /translation="MTEQLNTNQQNDEAQFMIQRIYIKDLSYETPNTPAVFQQQWEPE
                     LKLDLNTTTTQLDKNVYEVVLTVTTTVMNQKTTAFLVEVKQAGIFTIQGAAASQLDHL
                     LHSFCPSILFPYAREAITSQVIRGSFPQLVLAPINFDALYMQQLEEKQKATGAKDETV
                     SH"
     gene            10619..11608
                     /locus_tag="AC06IO_001151"
     CDS             10619..11608
                     /locus_tag="AC06IO_001151"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIO9874352.1"
                     /translation="MNKKTIAMLGAGSWGTAVAIHLAKIGHKTLLWSHNPQHVALMAE
                     QHSNPAYLPGIPFPENLIPSDNLIECVQSANYVIIAVPSHAFAEIINKIPKPTQGLAW
                     LTKGVDPASHELLSQLVASRFGVDFPIAVISGPSFAKEVARFLPTALTLASNNTNYQK
                     KMHQLFHHDNIRVYLSDDLIGVQLCGAVKNILAIACGISDGLGYGANAKAALITRGLA
                     EMTRLGLSMGARQDTFLGLAGVGDLVLTCTDDQSRNRRFGLLLGREVPIPEAEHQIGQ
                     VVEGKHNAAQICAIANKNRVEMPICEQINALLHGIVHAQEAVNNLMSRPAKEE"
     gene            complement(11694..12533)
                     /gene="murI"
                     /locus_tag="AC06IO_001152"
     CDS             complement(11694..12533)
                     /gene="murI"
                     /locus_tag="AC06IO_001152"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIO9874353.1"
                     /translation="MTRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYL
                     GDTARLPYGTKSPDTVKQYAMQMAKILVERRIKALVIACNTATTAALPHLQSMLTDIP
                     VLGVVAPGAAAAVSATKNHRIAVLATETTIASRAYHDLIIKNLPEAVVSSRACSVLVA
                     LAEEGMVTNAVAREALKHYLDDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQ
                     ATAISLYELLQNQNLHNDNLEVPPRINYLVTDSINRFQKVGEIFLGEQLSLSDIELID
                     AMG"
     gene            complement(12605..16114)
                     /locus_tag="AC06IO_001153"
     CDS             complement(12605..16114)
                     /locus_tag="AC06IO_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="interaptin"
                     /protein_id="HIO9874354.1"
                     /translation="MILEEYIRMAKNKEFFDALEEIAGSAKNDETLRNELAKVLDDIL
                     KTDPSDPDAFRKIIAEHQEFWDEHDPSLMEFSEGLFFGPSTEEYLESDDFLDSNDPIN
                     SFQKLHQLAAEQRVKLGLEKSDTDTLVAILKNNPEECRAYIESKKPALGNFSEGNVHG
                     WLKEEYTPTIPPKAINKSTGVLSDGAIKKIKEQASELLLLKLIKNCENQQLLKDLLTA
                     NSKTEAEHAANAIGFPTEGNGELSYPLSDEIEEALDDIIQELEEEAAKAGFDGYVQSL
                     SHDALLAKKNGLESTTAAGFKNSLDEPYKTYLPESEWERAQGVLGARYLQAVLSSGTQ
                     NLKDALNAKDANALIAELKKPALLGPHDYIDKAVTEENLGSLKKNMMKSFINNIKDET
                     NLKALDALKALDGAKNLDKFKEVLGKLGITPADWVKDTDLKDMKQWARARQFELEINR
                     VSSLGSGAHSKLMSSLTQLPVEKQREILAKPQQLRHLMNAYESHVAEHYLGKNASGIA
                     ELLTENKRLEGFRAIHNAEVARVLANFKPEITLNDKQVAAINQALTTANSNPNTYTQV
                     TDYKTLIDAIKTQSGSVNQKDFYNAFNLNDDGSAFTSSTPRKDEMSKQQQHNQHIYAE
                     YNSTSNPGNKKLLAVLLSIEKPVTFSKDIVNRFLRPLKDSETPQEYADRLFGENPTNP
                     ANKKFKDDLLRELTPTVFNEIKNDLRRQELLDTNPAHVMAAIKDLNTEFEAIKKITGP
                     IRTNADKLKFINDIDPVHLYNPTFQGTARSKAAQMKERYEGLSRDCELVVDQLRRQVI
                     ALEGHLKSLPQDSQFKAAGLTLEQKDEIKKLRTDLQTELDAVRKDLDFYKKIQGKLET
                     IVKEVDVAAKGKMHYYYNSEGIKRHPPVSRDQIPPLPNVPNPSLRSSTTATTGSNGRI
                     QEFLVGEKIPEGQIVVVDVSHKTAPKSGAPVETIGRYTQDNNVPDQVTSKKGEISKVP
                     GSKFEILQFPTQVPPPNPPSGDPLVEAKVNFSMAMAADILASLDSPPTKDKPIRLRGS
                     NPEELEYLYTALVILGEKNPKFKFNRDAIEVNSAVFHPDNVKGRLWGFSSNSLYSQVF
                     TNTGLTETQNIIQSKIKHMQQMTDEKFSPQKEREKVDSKVQEITDKQSKMKKELNPVH
                     KTTERTIEQEGPAPESPSTGMRK"
     gene            16236..16538
                     /locus_tag="AC06IO_001154"
     CDS             16236..16538
                     /locus_tag="AC06IO_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874355.1"
                     /translation="MRIKIILCGLILTNLTYAQGCIVGGQASQPRYVCFDGRTLDLTE
                     TGLVWNAKRGCFISSVPCCNYNATHYAYHPNPAKIGGAYARCRHDYPFTLGQMQTH"
     gene            complement(16542..17834)
                     /locus_tag="AC06IO_001155"
     CDS             complement(16542..17834)
                     /locus_tag="AC06IO_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HIO9874356.1"
                     /translation="MSPSESGELSIIQELSQRIVEAQRQIRILDSIKWDESVKQEFFK
                     FKGKKLPSIDKAYYDGKPLPFKVKDKQEEFRIILRDAQNQLGQYSTVTRLIRRQCEEY
                     SRAAQMLSLRGTPSFSEMAMELYGSPDDVFYVGGPRMCEMGSLLFDVLKNLQVQLQSQ
                     ADVKRYTPIQAQEILQERLEKFFDKHPGRVSVMVSDDMIADASAGADSIKLSQNAMFS
                     DRDLRYLEVHEGWVHVGTTLNGALQPNCFFLSKGSPSSSVIQEGLAVITEVVTFSSYP
                     GRMLKITNRVIALDKVTQGADFIDIYRYFLECGLSEEDSYNQSVRIFRGSTPTGGPFT
                     KDLSYAKGFVLIYNYIRFAIGQKRVDTIPLLFTGKLVLDDLPLLGELKEREILTDPVY
                     LPPPFQDLAALSAWMSFSLYLNQFDLNEIQKNFRFLLV"
     gene            complement(17844..18074)
                     /locus_tag="AC06IO_001156"
     CDS             complement(17844..18074)
                     /locus_tag="AC06IO_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874357.1"
                     /translation="MKKLSFIVLAALVLTACNSRYASNGENLYLQSRNGEALVVPPPL
                     TSANISNFYDLPQQTQDARVSIAPPVEDITTS"
     gene            complement(18089..18961)
                     /gene="dapA"
                     /locus_tag="AC06IO_001157"
     CDS             complement(18089..18961)
                     /gene="dapA"
                     /locus_tag="AC06IO_001157"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIO9874358.1"
                     /translation="MFSGSIVALVTPMRNDSVDVHHLRELVEFHIAKGTHALVAAGTT
                     GEAGTLSHSEKLLVIKTVIEQAKERVPVIAGTAMNATKDCIELTQQAMEYGAHAALIM
                     TPAYIKPTQEGLYLHYSHIAQSVAIPIILYNVPGRTACDMLPETVARLAKISNIIGIK
                     EATGQMTRLQQILRLCEGSIDVYSGDDLTAAQWLLAGAKGVISVTANVAAKLMAKMCD
                     LAMDDDQAGCLRIQEQLMPLHELLFVESNPIPVKWAMNKMGLIGGELRLPMTELSEKH
                     HQALEKVLKNLELI"
     gene            19173..19427
                     /locus_tag="AC06IO_001158"
     CDS             19173..19427
                     /locus_tag="AC06IO_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HIO9874359.1"
                     /translation="MKKIIPTLFFIGISTTMMVGCTNTQVGTAVGAAAGAGIGYGVSG
                     GSALGTAIGAGAGALVGGAIGQEQDRREYYYYSGYPYYYY"
     gene            19705..20487
                     /locus_tag="AC06IO_001159"
     CDS             19705..20487
                     /locus_tag="AC06IO_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HIO9874360.1"
                     /translation="MKLKNKVAIVTGAASGIGKEIALVYAKEGAKVAIADLNLDQANH
                     VAEEIKSSGGQAMAVAMNVTDEAQVNQGVDKVADHFGGVDVLVSNAGIQIIESVDKLA
                     FSDWKRMLAIHLDGAFLTTKACLKYMYASGNGGSIIYMGSVHSKEASVLKAPYVTAKH
                     GLLGLCKVVAKEGGTHKVRANVICPGFVRTPLVDKQIPEQAKELGISEEDVIKKVMLK
                     ETVDGEFTTTDDVAQTALFFASFESNALTGQSLVVSHGWFME"
     gene            20494..21654
                     /locus_tag="AC06IO_001160"
     CDS             20494..21654
                     /locus_tag="AC06IO_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIO9874361.1"
                     /translation="MTRTKKSARQIEYRHIACSFQGGGALGAYQAGVLHALNEVGYHP
                     HWYVGTSIGAINAAIAAGNSDKERIEKMYQFWESIATPSAVNESTLLDDKISRKIQHL
                     IAAHSALLLGQPGFFIPRFPSPDFGWHNSPDLLSYYDTSPLHNSLEKFIDFDRLNNNK
                     LTRISVGAVEVSSGMMTYFDSYKQKIGPEHIMASGALPPGFPAVKVDGKYYWDGGISS
                     NSPVNYILNHNNQKQLLCFMIHLFDSFGMVPTSMDEVLKRKKDIEFSSRFIKLINLHK
                     EIHDLRNTIHILSNHISPEEKSKPEIRECIKKGRPSAISLVRFLYEHDETELSSKDYE
                     FSKKSIHERIQKGYDDGKNAIKKSPWNQSVPMTEGIALYDMSAKDTMKKYQD"
     gene            21684..22589
                     /gene="motA"
                     /locus_tag="AC06IO_001161"
     CDS             21684..22589
                     /gene="motA"
                     /locus_tag="AC06IO_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HIO9874362.1"
                     /translation="MLIIVGYIVILLCVFGGFSLTGGHLAAIFQPVELLIIGGAAIGS
                     FIVGNNITVLKSVLKSLPATFRGQKSAKQASSDLLSLLYHLLSKARQQGLMSLENDID
                     EPDKSPIFTSYPNLMSNHHLIEFICDYLRLIITSNMQPFQLESLMDMEIESHHEEEMI
                     PANAITKLADAMPAFGIVAAVLGVVHTMESISLPPSELGVLIAHALVGTFLGILIGYG
                     FVGPIATAMEQNANQNQLMLQCIKVTILASLHNNPPIIATEFGRKVLFTSQRPSFNQL
                     NDEIKNAKTAQTTTQNSEATEATST"
     gene            22599..23537
                     /gene="motB"
                     /locus_tag="AC06IO_001162"
     CDS             22599..23537
                     /gene="motB"
                     /locus_tag="AC06IO_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HIO9874363.1"
                     /translation="MSEINDISDPINDDLEKRDKREKKEKTIHYTPIIRKIKKNAHKH
                     HGGSWKIAFADFVTAMMAFFLLMWLVASLNKAQKAGIAEYFKQPMKVALFGGESMGNR
                     KATIKGGGPNIEDTDGQVSATNKPLINESIAKDKEIIESQANEIKKLEELKSEINLSM
                     EKDPSLAGLKKQLLMDVVSDGLRIQLIDNQKKPMFDVGSDKINPEIEPILANIAKLLN
                     SVPNKITIQGHTDANPYHNPEELEYTNWELSAQRANAARRALIKAGMDENKVMTVTGY
                     SSTVLLDKKNPYNPENRRISIIVMKKGAENKIKVNK"
     gene            complement(23603..24040)
                     /locus_tag="AC06IO_001163"
     CDS             complement(23603..24040)
                     /locus_tag="AC06IO_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874364.1"
                     /translation="MKRIILFMIAWSLLNSAYSSHSLPSHASILFTLANTERSNSCPA
                     LLHNAKVVVDYDYNFERNMGLAHLRQLQSTKWSETLHPLGLSNYYAFMSDMKPKAVQI
                     EGGEVTIYRIIFHLYKNGDSRIYLMINQDGECIMSSDVVNVNL"
     gene            24321..25616
                     /locus_tag="AC06IO_001164"
     CDS             24321..25616
                     /locus_tag="AC06IO_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter"
                     /protein_id="HIO9874365.1"
                     /translation="MSSNESAAIVSPIVNQKLSQWKKQDTIWMLSLYGTAIGAGTLFL
                     PIDAGLNGILPLIIMTILAFPMTYYSHRALCRFVLSGSSSHNDITDVVEEHFGSFAGK
                     MLTILYFFAIYPILLMYSVAITNTTESFLINQMGITPPPRALLAIILILGLMGIVRLG
                     QEIIVKSMSVLVYPFATILFLLSLYLIPNWNNAIFIQSNSLHANGGHGLLMTLWLIIP
                     VMVFSFNHSPIISSFAVNQKQQYGSQADEKSSLIMKYSHLMMVFSVMFFVFSCVLSLS
                     PQDLLQAKQQNISILSYLANHFNTPIMAYIAPVIAFIAISKSFLGHYLGAKEGLSGII
                     VKSLKSTTGKTINKNSLHRIIEIFMILTCWVVATINPNILKMIETLGGPIIAVLLFIM
                     PMYAIAKVPAMKKYNNQISNVFVTVMGIISISAIIYGLI"
BASE COUNT         7780 a         5440 c         4785 g         7636 t
ORIGIN      
        1 tccaatcgtt cgggctgagg aggcatttat gctgtctcga agccttatat ggaatttaat
       61 gtttcatgcc aaggcttcga gacgatgctt aggcatctcc tcagcccgaa cgaaccctgc
      121 atatcgacta cttcttatat aatccttatc cataattcat gttaatgagg aagtttctct
      181 aaatgacatt tcgcattggt caacgctgga taagtaatac cgaatcacaa ctaggtttag
      241 gcattattac tgaagtagat ggcagacaag tttctatcag tttcccggct gctggggagg
      301 atagaattta tgctatagat aatgcgccat taagccgcat agtctataaa acaggtgaag
      361 aaatacttac caatgatcag caaagaataa aagtaacttc catccaggaa caagatggtg
      421 ttttactgta tacaggaaat gacgacttgg gaaatgtggc ccaagttagt gagctggcac
      481 ttaattgttt tatcaaactt aactcccctc aacaaaggct cttcagcgga ttattggaca
      541 aactaaatgc ttttaaactc agaattaaaa cattagagca tttaggccga ttacaacagt
      601 caaaagcaag aggcctatta ggttcacgaa ctaaccacct tatccaccaa atctatattg
      661 cttatgaagt tgcccaaaga tttgctccca gagtgatgct tgccgatgag gttggattgg
      721 gaaaaaccat tgaagcggga atgattttgc attatcaact gcatactggc cgtgcctcac
      781 gtgtattaat cattgttcca aattcattaa ttcaccaatg gtttgtcgaa atgttaagga
      841 ggttcaatct gcatttctct atcattggtc caaatcaata cgatgccatg ccatccacac
      901 ttgatgagtt ggaagataac gacaatccaa tcgagccagc attagaagat ggcaatctgt
      961 ttgaaggtga acaactggta ttaaccagcc tggatttcct tatggaaaat gaaacagcgc
     1021 gtcaacaggc attagctgcg caatgggatc ttctggtagt agatgaggcg catcatcttt
     1081 attggtcgga agactcacaa agtcccgaat acacgttcat tgaaaaatta tccgatcgaa
     1141 gcacaggcct gcttcttctt accgcgactc ctgaacaagt tggaatgaag agtcatttcg
     1201 ctcgtcttcg tctacttgat ccagcacgct tttatgattt ttcagccttt gtaaatgaag
     1261 aaaagcaata ccatgaaatt aataaaatag ttcaggatct catggattat aaggaaaaaa
     1321 atggcgtaga tgagttaaat ccccaactcc accctcattt aaaacaactc ttgggtaaag
     1381 aagcccctgc tggcattaat gcaacaatca aagagttgct tgatagattc ggcacaggtc
     1441 gagtactttt tcgcaatact cgtgcagcga ttaaaggatt tcccgaaaga agaatacacc
     1501 ctgtcgcatt aacatgccca gcgctttatt ccgagataat cagagaatcc aacttaatcc
     1561 atctttaccc tgaaactttg cttaataatg actcctggat agaccatgac ccccgagtgc
     1621 aatggcttgt taacaaaatt aatgaattac gtccggaaaa aattctggta atctgcgcaa
     1681 aggcaaaaac agccattagt ctggaacagt atttaaaatt aaaaacaggc attcgaagta
     1741 ctttatttca tgaaggactt accattatag agcgtgacag agccgccgct tattttgcag
     1801 aacaagaaaa tggtgcacag gttcttgttt gttccgagat aggaagtgag ggaagaaact
     1861 tccagttttc ccatcatttg gttttgtttg atttgccatt aaaccctgac cttttagagc
     1921 aacgtatcgg gcgtttggat cgtattggcc aaaatcatcc cattgaaatt catgtaccct
     1981 atttagcggg taccgcgcag gaaaagctat ttcgctggta tcacgaagga atcaacattc
     2041 ttcaacaaag ctgctctgtt ggcttttcaa tttttgaagt ttttgaaaat cgactgacac
     2101 ctgttttatc caatcccctg caggatacta gcgagcaatc attagagaag cttattgctg
     2161 atactcaatt gcatacccag catatcaagg aagcccttaa tgcaggaaga gatagattac
     2221 tagaattaaa ctcctgtaat tacactatag ggaaagaact cattgaagcg atagaagcag
     2281 aagaaaattg ccttgaactt gaaaattaca tgtcgcaagt gtatcaggag tatggaattg
     2341 atcaggaata tcattccgag aatgccgaaa tattgcgccc tggtaaccac atgaaaaccg
     2401 ctcattttcc tgggctaaaa gaagacggca tgaccgtcac ttattctcgc tctaaagccc
     2461 ttgttcggga agacatggag tttttaagtt gggaacaccc catggtttac gacactatgg
     2521 aaatgatcct ggagtcagaa cttggcaacg ccacagtgac tactatttca atcaaaagca
     2581 taaaaccggg taccctcttc ctggaaactt tttataccat taattcttcc gcccctaaat
     2641 acttacaact tgaccgattt attccttcat tgcctattcg aattttgatg gatactaccg
     2701 gcaaaaattt atcaggcatt ttaagttatg agcagcttaa taatctgtgc caaccactta
     2761 aacgtcatct gggttaccct gttattaaac aagttcgtga agagattgaa tccattctgc
     2821 aaagaaccaa caaaatcgca gaagatcaga tgaaattatt tattgaagaa gcaaaatcaa
     2881 tgatggagct aacgacttct caagaagtta atcgactgga atccttgcaa aaaattaatc
     2941 ccgccattcg ggatgaagaa attgagttct ttaaaaagaa aatttcagag agtaattact
     3001 tcattaatag ctccacttta aagcttcaag cgattcgggt tgtaatcaat gtcagttagt
     3061 taatcatccc ttgacaaatt gacacgataa taaaagaaat tgcttgtctt acaggctgga
     3121 cgctgatttc acaaaatcaa caactattag attattaacg gaaaatttct gtttataggc
     3181 ctgtcgcaac aggattgacg ccaatattag gattaatccc acaatcatcc agaattttta
     3241 ataaattatt tcctttacgg gttaatcgtt taatacagta ttcttccaga taactgctca
     3301 aaacataata atattcgtct aaaatcagaa tattaagaat tttctccgct ctttccagat
     3361 gacgcttgtt agcgtgatca caagcctgaa acaaaatgcc tcccaagatt gcagcaatac
     3421 ttgccttacg ttcaggactg gctaaataaa atcgttgtat gcattcatca agagccctgt
     3481 ctttatccca ctcgacccac tcatcatata gtgataaggc cacatcaggt ttaaaatttt
     3541 ctacgctggt cagctcctgc atagaatatg cgactggtac aacagcttgc acattagccc
     3601 acgaacaatt tccactaatt tgagaactaa ttggcatttg agcaaaagga agcagcccca
     3661 attgttgatt aatcatttga tgataatatc gtctgttttg ttttttatat aaaaaatcat
     3721 ggataaaatt gattgttagt gcctctggcc tggttattcg gtaaatatta acgcttcctt
     3781 cttgtaagct attttcacct ctgtcaattt ttgcccacca ctggtgataa cggacaaagc
     3841 ataaggcatg gccacgactc gccgcaggaa gaatcagcat aggggctttt aacaaagcag
     3901 caatacgttt gaaatgctgt tcgccaagcc tgggatgatg ttgatactgc aacagctctg
     3961 ccgcatcagt aaaagcatcc ataatattat gaatatatgg aaaataatcc cttaaatgcc
     4021 gagtcgaata actactgata aatctccgca gagagtcttt tacaacagca acagtgaatt
     4081 ccaaaataaa cccttcataa tcaagttcga tgaattcgcc tttcgcattg acaatatcca
     4141 catcgccttg caattcaaat cgatgaccaa tcaatttggc attaataaaa tcaagagcaa
     4201 aatcaagctt cgcgccatga tgatataaca actgctttat tgcatcttgg ccacgcaaaa
     4261 ccggataaac caacacacaa agaccattac gagtgtaggc attaggatca gcgccgtagg
     4321 ttaataagag tttggtcata tccaaatcat tattgtccac tgcccaatgt aatggagtgc
     4381 gtcctgtgac gtctggcttg ttgatatcca ctttgcgagc aattaactgc tccgctattt
     4441 taatttgtcg tgtaatggca cactctatga ggggagtaaa accatattca tcgatatcat
     4501 ccaaagattc ccctgcacgc agataagcat caaaatcagg cattctatga ctgataatat
     4561 cgtttgcaat actcatttag ggcccccggg gggtaggtgc tgtagtaaat tttggcatat
     4621 tacctaagcg taattgtttt tctttatcct gctctctttt ctcattatca aactccctcc
     4681 gggcttcagt attgagcggc ggttcggggt ttaaattagg atctattcca tcgaaacgct
     4741 gactgttcaa ttccgggtgt tgcaatatat tgttttgtaa ctccccttct ggagcaacct
     4801 tgatttcttc ttcagggtgt tcacgtgctt taacagaagc aaacctcctc tctccctgct
     4861 gttcagccaa acgtcttcgt ctttccatgg cttttgtttc agcaaccagg aaagtacttt
     4921 cactgcgatt ggacggcaat cgtgtgggta actcaacatg aaatactgag cccaacaaga
     4981 cataaacagc tgcagaacca ccatccttag gcttggcgct atgaaatgcc aatacctcat
     5041 tgatctgagg caaccaccga ttcaccagat ttttcattaa aggcggtttg tcctgagaat
     5101 tgtccgcacc atgaataatc agaatgcatt gtttgttatt ttttatttga gtttggataa
     5161 attgatataa gctcttacga gcagattcaa cgtttaaacc gtttaaatcc agttccgctt
     5221 cccagggagt ttttccattt tttaaatcat tgaagcgttt tgatgataaa ttgggtcgag
     5281 agtaagacaa tacagtactc gcgagcacta tttcttcgat ctcatcggat aaataatatt
     5341 ctttcgccat ctttttcaaa atttaacagt cataatagaa gtataactta ggatataaaa
     5401 agtatggcat tttttctaac ataacctgca tggccgatgg aggacataca agtttgctat
     5461 caagatactg aatctctgtt attgtggcaa agagagcttc acagaaactc tctttgcagt
     5521 atttcagcga tctgtaccgc gttagttgcc gcacctttgc gaatgttatc tgcgactatc
     5581 cacaaattta gtccacaagg atgagaaata tcctgtctta tgcgccctac aaaaacatca
     5641 tcatgcccga ctgcattctt aattgctgtg ggataacttg ctttagaaag attatccact
     5701 acagtaacgc cgggtgcctt tgccaaaagc gcgcgagcat cgtcagccgt caaaggcttt
     5761 tttaactcca gatgaaccgc ttcagaatgc ccataaataa caggaactct gacggctgta
     5821 gggttaacca taatgctgtc atcttccata atcttgcggg tttcccagac catcttcatc
     5881 tcttctcggg tataaccatt gtcttcgaac tgatcaatat gaggaagcgc attaaaagca
     5941 atttgctgag gataaacttg aacattagct ggcctcccat ttaaaagatc gcctacctga
     6001 gcaaccagct cgctgatggc ttttttgcca gtcccggaaa cagactggta agtcgccaca
     6061 ttaatacgac taataccaac agcgtcatag attggcttta gagcaacgac catctgaatg
     6121 gtagagcaat tagggttagc aataatgcct cttttagtat aatcagcgat tcgatgagga
     6181 ttcacttcag ggacaacaag aggaatatca tcctcataac gaaaacagga ggtattatca
     6241 accaccacgc acccagcagc cacagctttt ggtgcatact cttttgaaac agcgccgcct
     6301 gcagagaata aagcaagatc gactttgcta aaatcaaatt ctgctaaatc caaaacatcc
     6361 aattcctgat ctctaaaagt cactgtctta cctactgagc gagaactcgc taatgggtaa
     6421 agggacttaa ttggaaaatt tctttcttcc aaaacggtta aaaaggtttc tccaacagcg
     6481 ccggtggcac ctacgatagc tacattcaaa tgtctgctca ttatgacctc atcttaccat
     6541 caattaacaa tgtgctagtc aagttcaatt cctgcttgcg aagcagaaaa cgaactacga
     6601 tatggctata ttttgaaacg ttataattta ttatttacac tgcgctttat gccgtaaata
     6661 atgatccatt aacaccaaag ccatcattgc ctctgcaatt ggcacagccc ttatccccac
     6721 gcagggatca tgccgccctt tggttactac agtaacctct tcaccttcag tattaatggt
     6781 ctgacctgga gttgtgatgc ttgaagttgg ctttaaagcg atgctcactt cgatgggttg
     6841 tcctgttgct atgcctccta aaataccacc ggcatgatta ctcaagaatc ccttggcagt
     6901 catctgatct ctatgatgac ttcctaactg ctccactgca ttaaaaccag ctccaatttc
     6961 aacaccttta acagcattaa ttgacatcat tgcataagca agagtagcgt ccagtttatc
     7021 aaatacaggg tctcccaatc ctgttggcac tccccgtgcc agtatcttta cccgagcacc
     7081 aacggaatca ccctggcgtc ttaaacgatc tacataatca gccagctctt gaatttgctt
     7141 attattagga caaaaaaagg gatttttatt gatttcattt tcattttcaa accgtaactt
     7201 caaatcgccc atttgttgta aataaccaat aatatccagg tttaaataac gctttaaata
     7261 caatctggca atagcaccgg cagcgactcg tgcggctgtc tcacgagcgg aagaacggcc
     7321 tccccctcta taatctctat gcccatattt ataatgatag gtaaaatcag catgtcctgg
     7381 acggaacaaa tttttaatgt cttcataatc ccgggaacgc tgatcagtat tctgtatcag
     7441 caatgcaatg ggagctcctg tcgttttccc atcaaataca ccagaaagaa tctgtacctt
     7501 atcttcctct cgtctctggg tagtatattt agattgacct ggtttacgtt tgtctaaaaa
     7561 aggttggata tctgcttcag ttaaagacat gccgggagga caaccatcaa ctacacatcc
     7621 gatagcagga ccgtggcttt caccaaaggt tgttacagta aataacgctc caaaggtatt
     7681 ccctgacatg ccttactcgc ttatttaaaa tagtcttcta attgctgttt tgttaataaa
     7741 aacacccctt gcccgccgtt gctcatttca agccaggtaa aaggaaccat aggataagtc
     7801 tcacataaag cctcttcact attacctact tccactacca aaataccatg ttcacttaaa
     7861 taagcatggg cattctttaa aattttttca ataatagcca agccattatt tcctgtttcc
     7921 aatgccaaaa ccggttcatg gcggtattca tccggcaggg tctgcatctc ttccttcccc
     7981 acataaggag gattgctcac gatcaaatca tattgtttac ctgacagtgc cgtaaaacaa
     8041 tccgattcaa tcagtgccaa ttgatcgcct acatcatgac gttcacaatt gatagtagcg
     8101 acagccaatg cttgatttga aatatccaca gcatccacct gagcctcagg gaatgcataa
     8161 caacaggcaa tcgctataca accactcccg gtacataaat ccaaaacatg atgcacttgt
     8221 tctgcttcta tccaagggga aaattgatta ttgattaact ctcctattgg tgatctgggt
     8281 ataagcactc gttcgtcaac ataaaaaggc aaatcgcaaa aataggcttc cttaattaaa
     8341 taaggtacgg gtactctttg attaattcgt ttatccaatt gattacaaag atgtttgcgt
     8401 tcactggttg ttaaacgtgc attcaacaaa aaaggatcga catcgtatgg taaagacaag
     8461 cttctcaata tcagggatct catgtcatcc caagcgttgt cagttccgtg gccataaaat
     8521 aattgcgcgt tttctgcaca agacaaacta aaccgtaaaa aatccagaat agtaaccagt
     8581 tcttctgttt ccttgctaaa ataactactc acaagcgtat caaccataac catagaaaaa
     8641 gtacaaattg tacactaaat taaaatctat gtcctgtatt ctttgttaag ggggtgcgat
     8701 ttaagacgcc ccttcgcatg gcttgatttt cacctaacct aaagaattac ttcaaattaa
     8761 gttataatta aatatattct tttgatgcct gttaaaaaat gactgatgat ttcctgtctg
     8821 atgaggataa agctctattt cgtcatcata tgcgttctgt aaaaccatta aacgaaaaaa
     8881 caaaacgcgt aaaaaacccc acgcaaccca ctcatgaaaa aaaaaccaca actttcaaga
     8941 gtcagcccgt acccaaagaa gaatattttt tatctgattt tattcgtgac actgttcaac
     9001 ccgatactat tttatctttt tcagaaccag gcattccaag ccagagattc cgtgcactaa
     9061 aaaacggaca aattccatgg gaagccaagc tggatttaca tggattaaaa gctgaagcag
     9121 ctcgccaagc gctttatgat tttatccaga ctcagtcact caataataaa cgctgcttac
     9181 tcattattca cggcaaagga ggccatcaag gcgctccccc ggtaataaaa aacctaatca
     9241 atcgctggct gccgcaattt agtcaagtcc tggcattcca cagcgctctt ccaaaggatg
     9301 gaggattggg ggctgtgtac gtcttattaa aaagaaacag agaaaaggat gaacaggaca
     9361 attactagta acactttact tgcgttaaag cagtaatctt aggataattc gtcaaattaa
     9421 aaaatatctt gagtacagct atggaaaaat taggtcagtt catcatcaat cattggcaac
     9481 tctggctcgc ctttgttgtg gttgtatttt taattttcat taatgaaata ctctctcaaa
     9541 gaaagaaagc caaagaagtt tcaccgcaat ccctggttga tttaatgaac aatggaaatg
     9601 ccatcgtaat cgacatacgc gacaaagaat cattcaaaaa tggccatatc attaattcaa
     9661 tgaacgtaaa tcctgatgat tttgaacaac aaaaaatgag caagtacaaa gataaaccca
     9721 ttatcctggt ctgcgcacga ggcttacaat ctcgaaccct agccatgaaa atccgcaatc
     9781 aaggttacca acctctggtt ttgagtggag gaattcaagc ctggcaaaat gctgatcttc
     9841 ctttagtgaa gggtaaatga acagatgaat gaagtcatac tctacactac aggctattgt
     9901 ccttattgca tcaaagcaaa agaactacta gatagaaaaa aggttatcta taccgaaatc
     9961 cgcgtcgatt tacagcctga attacgtgaa gaaatgatac aaaaaagtgg ccggcgtaca
    10021 gtgcctcaaa ttttcattaa tggccaggca attggtggct gcgatgattt atatgccctg
    10081 gaagcccagg gaacattaaa tgaactttta aaaaaatagg aataaaacat gactgaacaa
    10141 ctgaatacca atcagcaaaa tgatgaagct caattcatga ttcaacgaat ctatatcaaa
    10201 gatttatcct atgaaacacc aaatacaccg gctgtttttc aacaacaatg ggaaccagag
    10261 ctcaaactgg atctcaatac aacaactact caactggata aaaacgttta tgaagtggtt
    10321 ttaactgtta caaccactgt gatgaatcaa aaaaccacag catttttggt tgaagtaaag
    10381 caagcgggca tctttactat ccaaggcgcc gcagcaagtc agttggatca cttgcttcat
    10441 agcttttgcc ccagcatcct gttcccctat gctcgtgaag caattacctc gcaagtcatt
    10501 cgtggcagct tcccccaatt agtactcgca ccgattaatt ttgatgcttt atatatgcag
    10561 cagctggaag aaaaacaaaa agcaactgga gctaaagacg agactgtttc tcactaagat
    10621 gaataaaaaa accattgcca tgttaggtgc cggctcctgg ggcactgctg tcgcaattca
    10681 tttagctaaa atagggcata aaacgctgct gtggagtcat aacccacagc atgttgccct
    10741 catggctgaa cagcattcaa atccagctta ccttcctggt ataccttttc cagagaattt
    10801 gattccttct gataatttaa ttgaatgcgt tcaatcagcg aattatgtca tcatagcagt
    10861 tccttctcat gcctttgcgg aaataatcaa taaaatccct aaaccaaccc aaggattggc
    10921 gtggcttacg aagggtgttg atccagcgag tcatgaatta ttaagccaac tggttgccag
    10981 tcgatttggg gttgattttc ccatagcagt tatttcaggc ccatcgtttg ccaaagaagt
    11041 ggctcgcttc ctgcctacag ctctcacttt ggccagtaac aatacgaact atcaaaaaaa
    11101 aatgcatcaa ctatttcatc acgacaacat tcgtgtctat ttaagtgatg atttaatagg
    11161 tgtgcaatta tgtggcgctg ttaaaaatat cctggcgata gcctgtggaa tcagtgatgg
    11221 cttagggtat ggcgctaatg ccaaggcagc gcttattacc cgtggacttg ctgaaatgac
    11281 tcgtttggga ttaagcatgg gagcacggca agatactttt ctaggcttgg ctggtgtagg
    11341 ggacttggta ttgacttgta cagatgatca atcgcgcaat cgccgctttg gattgctctt
    11401 gggtcgtgaa gtgcctattc ctgaagccga acaccaaatt ggccaagtag ttgaagggaa
    11461 acacaatgcc gctcaaattt gcgctatagc caacaaaaat agggtggaaa tgccaatttg
    11521 tgagcaaatt aatgcgctat tgcatggtat tgttcatgcc caagaggccg tgaacaactt
    11581 aatgagccgt cccgccaagg aagaataacc tttgcccttg caaagaatca tattaaatta
    11641 ggtttagtgc accaggacac caggacgcag gtgatactgg tcgattgtcg acattaaccc
    11701 atagcatcaa tcaattcaat atcactcaaa ctcaattgct cacctaaaaa tatctcgcct
    11761 accttctgga acctattgat agaatctgtt accaaatagt tgattctggg gggaacttcc
    11821 aaattgtcat tgtgcagatt ttgattttgc aataattcat aaagagaaat tgcagtcgcc
    11881 tgagcagaat ctacaattgc gacatctttt ggcaataagc tggatagtaa gggtttaaat
    11941 actggaaagt gagtgcaccc caataacaca gcatcttcat ccgaaaaatc gtcgagataa
    12001 tgcttcaatg cctcacgagc aactgcatta gtcaccatac cttcctctgc caaggcgact
    12061 aaaacactac atgcccgact gctgacaact gcctcaggca aattctttat gatcaaatca
    12121 tgataggctc tggaagcaat ggttgtttct gtagccagaa cagcaatacg atgatttttg
    12181 gtagctgaca ccgcagcggc tgcaccaggt gccaccacac ccagaaccgg aatatcggtt
    12241 aacatgctct gtaaatgagg taaggcagcc gtcgttgcag tattacaggc aatgactaaa
    12301 gcttttatgc ggcgctcaac caaaattttt gccatttgca tagcatattg tttgactgtg
    12361 tcagggcttt ttgtgccata aggtaaacgg gcggtatctc ccaaataaac aaaagattca
    12421 tgaggtaatt tatcccgcag agcccttaaa accgtaagtc cccccatgcc tgaatcgaat
    12481 acaccaatag ctaaatcctt gttagagttc aattgattac tccttcttgt catttatttc
    12541 ctggctctct tatttggcaa aatagcttcg ttctaccaaa aatgagagcc aaagtcatta
    12601 ttatttactt acgcatccct gtactcggac tttcaggcgc tggtccttct tgttcaatgg
    12661 ttctctcagt tgttttatgc accggattca gttccttctt catcttgctt tgtttatcag
    12721 taatctcttg tactttggaa tccacttttt ctctttcttt ctgaggacta aatttctcat
    12781 cagtcatttg ttgcatatgt tttattttag actgtattat gttttgggtc tcagttaatc
    12841 ctgtgttagt aaatacttgg ctatataatg aattgctact aaatccccac aaccgccctt
    12901 tcacattatc cggatggaat actgcagaat tcacctcaat cgcgtcacgg ttaaacttga
    12961 attttggatt cttttcacca aggatcacca gggcagtgta caaatattcc agttcttcgg
    13021 gattagatcc gcgtaatcgt atcggtttat ccttggttgg tggactatcc agactcgcta
    13081 aaatatcagc cgccatcgcc atggaaaaat tgactttagc ttctaccaga ggatctccac
    13141 ttggagggtt tggaggtgga acttgggtcg ggaattgtaa aatctcaaat ttacttccgg
    13201 gaaccttgct tatttctcct tttttgctcg ttacttgatc aggaacattg ttatcctgag
    13261 tataccgtcc tatcgtttcc acaggagcac cggatttcgg tgctgtttta tgagatacat
    13321 caacgacaac aatctgccct tccggtattt tttcaccaac aagaaattct tgtatccttc
    13381 cgttagaccc agtagtagcg gttgtactcg atctcagaga aggattagga acattaggta
    13441 atggcggtat ctgatcacgt gaaacaggag gatggcgttt gataccctca gaattgtagt
    13501 agtaatgcat cttccctttg gctgccacgt ccacttcttt tacaatggtt tctaactttc
    13561 cctgtatttt tttataaaaa tccaaatcct tgcgcaccgc atccaactca gtttgcaaat
    13621 cagtacgcaa ctttttgatt tcgtcttttt gttccagggt caagccagca gccttaaatt
    13681 gactatcttg aggcaagctc ttcaaatggc cttcaagagc aatcacttga cgtcgcaatt
    13741 gatcaacaac cagttcgcaa tccctggata atccttcata tctttctttc atctgagctg
    13801 ctttcgatct ggcggtgccc tgaaaggttg ggttgtataa atgaactgga tcaatatcat
    13861 tgataaactt taatttatct gcgtttgttc ttattggtcc agtgattttt ttaatagcct
    13921 cgaattcggt attcaaatcc ttgattgctg ccatcacatg agctggattg gtatctaaca
    13981 gttcctgcct cctgagatca ttttttatct cattaaatac agttggtgta agctcccgca
    14041 acaaatcgtc tttaaatttt ttatttgccg gattagttgg attttctcca aataatctat
    14101 cagcatactc ttgaggagtt tcgctatcct tcaaaggacg taaaaaccga ttgactatat
    14161 ctttggaaaa ggtgacaggt ttctctatac tcaacaggac agctaataat tttttattac
    14221 ctggattaga agtactgttg tattcagcat atatatgttg gttatgttgt tgctgcttac
    14281 tcatctcatc ttttcgagga gttgagctgg taaaagcact gccatcatcg tttaaattaa
    14341 acgcgttata aaaatctttt tgatttaccg agccagattg agttttaatg gcatcaatca
    14401 aggttttgta gtcggtgacc tgggtatagg tattggggtt agaattggcg gtggttaatg
    14461 cctgattaat agcagcgact tgcttatcat ttaaagtgat ttcaggttta aaattagcca
    14521 aaaccctggc tacctcggca ttgtgaatag ccctaaatcc ctcgagtctt ttattctctg
    14581 tcaagagctc tgcaatgccg gatgcgtttt ttccaaggta atgttctgct acatgagatt
    14641 cataagcgtt cattaaatgg cgtaattgtt gtggcttggc caaaatctct ctttgttttt
    14701 cgactggaag ctgggttaat gagctcatta atttcgaatg agccccgctt cctaaagaag
    14761 acactctatt tatttcaagt tcaaactggc gagctcttgc ccattgcttc atatctttca
    14821 aatcggtgtc tttcacccaa tccgctggag taatacctaa cttcccgagt acctctttaa
    14881 atttatccaa atttttagcc ccgtctaatg cttttaaagc atccaaggcc tttaaatttg
    14941 tttcatcctt gatattatta ataaagctct tcatcatatt ttttttgagc gagcctaaat
    15001 tttcttctgt caccgcttta tcaatgtagt catggggtcc caaaagggca ggttttttta
    15061 attcagcaat caatgcattg gcatctttag cattgagagc atccttgaga ttttgggttc
    15121 ctgatgataa aaccgcttga agataacgag ctcccaatac tccttgggct ctctcccatt
    15181 ccgactccgg taagtacgtt ttgtaaggtt catccaaact gtttttaaac cctgcagctg
    15241 tcgtactttc taacccgttt tttttggcta atagcgcatc atgagataat gattggacat
    15301 aaccatcaaa tcctgccttg gcagcttctt cttccagctc ctgaattatg tcatccaaag
    15361 cttcttctat ttcatcactt aagggataag ataattctcc atttccctcg gttggaaaac
    15421 ctatggcatt tgcggcatgc tctgcttcag ttttactatt agctgttagc aaatctttca
    15481 ataattgctg gttttcacag tttttaatca gcttaagcaa taataattcg cttgcctgtt
    15541 ctttaatctt tttgattgcc ccatcagata aaacgccagt ggatttgtta atagctttcg
    15601 gaggaattgt cggggtatat tcttctttta accaaccatg cacattccct tcactaaaat
    15661 tacccaaagc tggttttttg gattcgatat acgctctgca ttcttcaggg ttatttttta
    15721 aaatagcaac caaagtatca gtatctgatt tttccaagcc aagcttgact cgttgttcag
    15781 cggctaattg atgtagtttt tgaaaactat ttattgggtc attagaatcg aggaaatcat
    15841 ctgattctaa atattcttct gtgcttggtc caaaaaataa tccttcacta aactccatta
    15901 agctgggatc atgctcatcc cagaactcct gatgctcagc tattatcttt ctaaaagcgt
    15961 caggatcaga aggatcggtt ttcaatatat catctaaaac ttttgcaagc tcattacgaa
    16021 gagtttcatc attttttgct gatcccgcaa tttcttccag cgcatcaaaa aattctttat
    16081 ttttggccat acggatgtac tcctcgagaa tcattatgtg ttcattttaa gacaaaaaac
    16141 ttaagttatc cttaaatttt gccgatatta ataccacaga tggaaaccta agctagcttt
    16201 ataattagaa gtatagcaag gataaggtga atagaatgag aataaaaata attctttgtg
    16261 ggttaattct aacaaacctc acctatgccc aaggctgtat tgttggcggt caggcctctc
    16321 aacccaggta tgtctgtttt gatgggcgaa cgttggatct gactgaaaca ggccttgtat
    16381 ggaatgccaa aaggggatgt tttatcagct cagtaccttg ctgtaactac aatgccactc
    16441 attatgccta ccacccaaat ccggccaaaa taggcggggc ctatgcccgt tgcaggcacg
    16501 actatccctt tactcttggc caaatgcaaa ctcattaatg tttaaaccaa caaaaatcga
    16561 aaattctttt gaatttcatt taaatcaaac tggttcaaat acagagagaa actcatccat
    16621 gcgcttaatg cagccaaatc ctggaaagga ggtggaagat acactggatc cgttaaaatc
    16681 tcgcgttctt ttaattcacc tagcaaaggt aaatcatcca aaaccagctt gccggtaaat
    16741 aataatggga ttgtgtcgac tcttttctgc cctatagcaa agcgtatgta attgtaaatt
    16801 aatacaaatc ccttggcata ggataaatct ttggtaaatg gaccacctgt cggtgtgctg
    16861 cctctaaata tacgtacgga ctggttatag ctgtcttctt ctgacaatcc gcactccagg
    16921 aaatagcgat aaatatcaat aaaatctgcc ccttgagtca ccttatccag ggcaatcacc
    16981 cggtttgtaa ttttcaacat ccgacctgga taagaagaaa atgtcacgac ttcagtaatg
    17041 actgccaagc cctcctgaat cacgctgctg gaaggagaac ctttagacag gaaaaagcaa
    17101 ttcggttgca aggctccatt gagagtcgtt cccacatgaa cccagccctc atgaacttcc
    17161 aaatagcgta aatctctatc gctaaacata gcgttctgac ttaacttgat gctgtcagct
    17221 ccagcagaag catcagcaat catatcatca ctgaccataa cactcactcg gccaggatgc
    17281 ttgtcaaaaa atttctctaa tcgctcctgt aaaatttcct gagcctgtat cggagtgtaa
    17341 cgcttgacat cagcctgaga ctgcaattgg acctggagat tctttaatac atcaaacagc
    17401 agggaaccca tttcacacat acgcggtcca cccacataaa acacatcgtc cgggctacca
    17461 tacaattcca tagccatttc gctaaatgaa ggggtacccc gcaaggataa catctgggct
    17521 gcgcggctat actcttcgca ttgacgtctg attaggcgag ttactgtgga gtattgccct
    17581 aattggttct gtgcgtcacg caaaatgata cgaaactctt cctgcttgtc tttgacttta
    17641 aaaggcagtg gcttaccgtc ataatatgcc ttatcaatag aaggaagttt ttttcccttg
    17701 aacttaaaaa attcctgttt tactgattca tcccatttaa tactatcaag aatcctgatt
    17761 tgacgctgcg cctccacgat acgctgagac aactcctgta ttattgacaa ctctcctgat
    17821 tctgatggcg acatgatgaa tccttatgaa gttgtgatat cttccaccgg aggcgctata
    17881 ctcacccgcg catcctgagt ttgttgaggt aaatcataaa aattactaat attggcacta
    17941 gtcaatggag gtgggactac caaagcctcg ccattacggc tctgtaaata taaattttct
    18001 ccattactgg cataacgaga attacatgct gttagtacca gtgctgccaa gactataaaa
    18061 ctcaattttt tcacaacaaa accctcagtt atatcaactc taaattcttc aacacctttt
    18121 ccaaagcctg atgatgtttt tcagacaact ccgtcatcgg taatcttaat tcccctccaa
    18181 ttaatcccat tttgttcata gcccatttta ccgggattgg attcgactcc acgaacaaaa
    18241 gctcatgtaa aggcattaat tgctcttgaa tacgcaaaca tcctgcttga tcatcatcca
    18301 ttgccaaatc acacattttt gccatcaatt tagcagccac attagccgtg actgaaataa
    18361 cacctttagc cccggccaac aaccattggg cagctgtcaa atcatcgcca ctgtaaacat
    18421 caatgctccc ttcgcataac cgcaaaatct gctgtaaacg agtcatctgt ccggttgctt
    18481 cctttattcc tattatattc gaaattttag ccagtcttgc caccgtttcg ggtaacatat
    18541 cacaggctgt tcttcccgga acattatata aaataatcgg tatcgctacg gattgggcaa
    18601 tatgactgta gtgcaaatac aacccttctt gagtgggctt gatataggct ggtgtcatga
    18661 ttaaagcagc atgcgcccca tattccatag cttgctgggt tagttcaata caatccttgg
    18721 tcgcattcat agcagtaccg gcaatcacag gcactctttc ttttgcttgt tcaatgactg
    18781 ttttgataac caaaagtttt tcgctatgtg atagagtacc agcctcacca gtcgtgccag
    18841 ctgcgactaa ggcatgagtt cccttagcta tatgaaattc aaccagttct cgcaaatgat
    18901 gaacatcgac gctatcattt cgcatggggg ttaccaaggc tactatactt ccactgaaca
    18961 tgttgccctc aaaaattaat gatccatgat cgtatattac tggaaaacag acacccgagt
    19021 aaatttttat ataacacttt agtcaactgg aatctctatt agcaatcatt atcttcaatc
    19081 ccaaatacat atttgtttaa attttttaca tatttagctt attttgtact atccttgata
    19141 tgaaggcaac aacttacaaa aggaagttta ctatgaaaaa gattatacca acgttgtttt
    19201 tcatagggat atcaaccact atgatggtag gttgcacaaa tactcaagtt ggaacagcag
    19261 ttggagcagc tgcgggcgct ggtattggct atggagtttc tggaggatca gcattgggaa
    19321 ctgcaattgg agcaggagct ggtgcattgg taggcggtgc catcggtcaa gagcaagaca
    19381 gacgtgagta ttattactac agtggctacc cctattatta ttattaattc tgacttgtcg
    19441 actagatata ttccttaatc attttagcga tcataagaac ctggtctttt ctagatagag
    19501 aaagggccgg gatagtttct tgatttgggt aaaaccaaaa ctcttggtga aatggtacag
    19561 aaaagaagca attacctccc ttaaagacaa ctctctaaaa aatttaaaca attcaccaaa
    19621 gatttttgtt agcaaataat aaggcattat taaaaatcca ttgctataat caattcatgg
    19681 aaaacaatga caaggatagt aaagatgaaa ctgaagaata aagtcgctat tgtcacagga
    19741 gcagcaagcg gaattggtaa agaaattgcg cttgtttatg ctaaagaagg agcaaaggtt
    19801 gcaatcgctg atctgaatct tgatcaggca aaccatgtag cagaggaaat caaatcatca
    19861 ggcggacagg caatggctgt agccatgaat gtaactgatg aagcacaagt caatcagggc
    19921 gtggataaag tagccgatca ctttggaggg gttgatgttt tagtcagcaa tgccggtatt
    19981 caaatcatcg aatcagtaga taaattagcc ttttcagact ggaaaagaat gcttgccatt
    20041 catttggatg gtgccttttt aactactaaa gcttgcttga aatacatgta tgcctcaggc
    20101 aatggcggca gtattattta tatgggctct gtccattcca aagaagcttc tgtactcaaa
    20161 gcaccttatg ttactgccaa acatggtcta ttgggtttat gcaaagtagt tgccaaggaa
    20221 ggtggaactc ataaggtaag agccaatgtc atttgcccgg ggtttgtccg tactcctctt
    20281 gttgacaaac aaattccaga gcaagcaaaa gaattaggca tcagtgaaga agatgtcatc
    20341 aagaaagtga tgctaaaaga aacagttgac ggggaattca ccaccacaga cgatgtagca
    20401 caaaccgctt tattttttgc atcatttgaa tcgaatgcgc ttacaggaca atcattggta
    20461 gtcagtcatg gttggttcat ggagtaaaca accatgactc gaactaaaaa atctgcaaga
    20521 cagattgagt atcgtcatat tgcatgcagc tttcagggag gaggtgcttt gggtgcctat
    20581 caagctggag tattgcatgc tttaaatgaa gtgggatacc accctcattg gtacgtggga
    20641 acctcaattg gagcaattaa tgcggcgatt gctgcaggta actctgacaa agagcggata
    20701 gaaaaaatgt atcaattttg ggagtctatc gctactccgt ccgcagtcaa tgaatcaact
    20761 ctcctcgatg ataagataag tcggaaaatt caacatttaa ttgctgctca ttcggcttta
    20821 ctcttgggac agccaggttt ttttatacct cgtttccctt cccctgattt tggttggcat
    20881 aacagccctg atttattaag ttactatgat acttctcctt tgcataacag tttggaaaaa
    20941 tttattgatt ttgatcgtct gaataacaat aaattaaccc gaatcagtgt aggagccgtt
    21001 gaagtgagta gcggaatgat gacttatttt gattcctata aacaaaaaat aggacctgaa
    21061 cacattatgg caagcggtgc gcttcctccc ggcttcccgg cagttaaagt cgatggtaaa
    21121 tattactggg atggcggtat atcaagtaat tcaccagtca attatatcct gaatcataac
    21181 aaccaaaagc aactgctttg ttttatgatt catttgtttg attcttttgg gatggttccc
    21241 acctcaatgg atgaggtatt aaaacgtaaa aaagacattg aattttccag tcggtttata
    21301 aaattaatta accttcacaa ggaaattcat gatttaagaa acacaattca cattttatca
    21361 aaccatattt ctcctgagga aaaatcaaaa cctgaaataa gggaatgtat taaaaaggga
    21421 cgtccttccg ctatttcgct ggttcgattt ttatatgagc acgatgaaac ggaattgtct
    21481 tcaaaagatt atgaattctc caaaaaatct attcatgaac gcattcaaaa aggttatgat
    21541 gatggtaaaa atgcgatcaa aaaatcgcct tggaatcaat cagtcccaat gactgaaggt
    21601 atagcactgt atgacatgtc tgccaaagac actatgaaaa aatatcaaga ctaactggac
    21661 accagatact ataggatagg gatatgttaa ttatagtagg ttatattgta atactactct
    21721 gtgtatttgg tggattcagc ctgacaggcg gtcatctcgc cgctatattt cagcctgttg
    21781 aattattgat aatcggaggg gccgccatag gttcctttat cgttggtaat aatattaccg
    21841 ttttaaaatc tgttctaaaa tctctgcctg ctacatttcg cggccaaaaa tcggcaaagc
    21901 aagcctccag tgatttgtta agccttctat accatttact cagtaaagca aggcaacaag
    21961 ggttaatgtc gctcgaaaat gacattgacg agccggataa aagcccaatt tttaccagtt
    22021 atcccaattt aatgagtaat catcatttaa tagagtttat ttgtgattac ctgagattga
    22081 ttattacttc caacatgcag ccttttcaat tggaaagctt gatggatatg gagatcgaat
    22141 ctcaccatga agaagaaatg atcccggcca atgccatcac caaactggct gatgccatgc
    22201 ctgcttttgg tatcgtcgca gcagtactag gtgttgtcca taccatggaa tccatcagct
    22261 taccgccctc tgaattaggt gttttaatcg ctcatgcgtt agtaggaacc tttttaggta
    22321 ttttaattgg ctatggtttt gttggaccta tcgctactgc tatggaacaa aatgccaatc
    22381 aaaatcaact gatgctgcaa tgtataaaag ttaccatcct ggcaagtttg cacaataacc
    22441 ctcctattat tgctactgaa tttggacgta aagtattatt tacatcgcaa agaccttcat
    22501 ttaaccaatt aaatgatgaa ataaaaaatg ccaaaaccgc tcagacaaca actcaaaact
    22561 ctgaagcaac agaagctacg tccacttagg ttacagccat gagcgaaata aacgacatca
    22621 gcgatccgat caacgacgat ctggaaaaaa gggacaagcg ggaaaaaaaa gaaaaaacca
    22681 ttcactatac acctatcatc agaaaaatta aaaaaaatgc tcataagcat catggaggct
    22741 cttggaaaat tgcctttgct gactttgtaa cggccatgat ggcctttttt ctactcatgt
    22801 ggttagttgc ttccttaaac aaagctcaaa aagcgggtat tgccgagtat tttaaacagc
    22861 caatgaaagt ggcattgttt ggcggggaaa gcatggggaa tagaaaagca acgattaaag
    22921 gaggaggccc taatattgaa gacacagatg gccaggtttc tgccaccaat aaacctttaa
    22981 tcaacgaaag catagcaaaa gataaagaaa ttattgaatc tcaagctaat gaaataaaaa
    23041 aacttgaaga gctgaaatca gagatcaatt taagtatgga aaaagatcca tcgcttgctg
    23101 gtctaaaaaa acaactcctg atggatgttg tgtccgatgg cttgcgtatt caactgatag
    23161 ataatcagaa aaaacccatg tttgatgtag gctctgataa aatcaatccg gaaattgaac
    23221 ccatactcgc caacatcgca aaactactca attcagtgcc taataaaatc actattcaag
    23281 gccacaccga tgccaatcct tatcataatc ccgaagaatt ggaatacacc aattgggaat
    23341 tatctgcaca aagagcgaat gcagcaagac gcgcattgat aaaagccgga atggacgaaa
    23401 ataaagttat gacagtcaca ggttattctt ccactgttct tctcgataaa aaaaatccct
    23461 acaatcctga aaatcggcgc attagcatca ttgtcatgaa aaaaggggca gaaaacaaaa
    23521 tcaaggtcaa taaataattt ggcattcgac caaggctagc tccgtttctt agtggttttc
    23581 attaaaatta atactattta ctttacaaat taacattcac aacatcagaa ctcataatgc
    23641 attcgccatc ttgattaatc ataagataaa tcctgctatc gccattttta tacaaatgaa
    23701 aaataattcg ataaatggtt acctcacccc cttcaatctg aaccgctttg ggcttcatat
    23761 ccgacataaa tgcataataa ttacttaaac cgagcggatg tagtgtttcg ctccacttgg
    23821 tcgattgcaa ctgtcttaag tgagctagcc ccatatttcg ctcaaagtta tagtcataat
    23881 caacaacgac ctttgcatta tgcagcaaag ccgggcatga attactacgc tctgtattgg
    23941 caagggtaaa gagaatgctg gcatgggatg gaagtgagtg agaagaatag gctgaattca
    24001 ataaactcca tgcgatcata aacaaaatga ttcgtttcat aactactcca taaattaaac
    24061 ctagttcgag aaacattctt attacaatac gaattcattt gcaacaattt aaaagaataa
    24121 gatcggaatg agcttcatga attgttaaga aggcaaggcc attttcctcg ctccttacct
    24181 taaattaatc tcttgctttg ttcatttcag ttggatatga tttagttcgc aattgttgaa
    24241 atattgtctt tgtagtgtta atattgatca acttgcgagg cgctatttta ttagttagta
    24301 ttaaaacgag gatgtttttc atgtcatcaa atgaatctgc agctatagta tctccgatag
    24361 tcaaccaaaa actatcgcaa tggaaaaaac aagatacgat ttggatgtta agcctctatg
    24421 gcacagcaat tggtgccgga actcttttcc ttcctataga tgctggtctt aatggcattt
    24481 tgcctttaat cattatgaca attctggcct tccccatgac ttattattct catagggctt
    24541 tatgtcgttt tgtcctttca ggctcgtcct cacacaatga catcactgat gttgtagaag
    24601 aacactttgg ctcatttgcg ggtaagatgc tgactatcct gtattttttt gcgatttatc
    24661 caattttgct tatgtatagt gtcgcaataa caaataccac ggaaagtttc ctgattaatc
    24721 aaatgggaat tacccctccg ccacgagctc ttttagcaat catattaatt cttgggttaa
    24781 tgggtatcgt gcgtttagga caagaaatta ttgtcaaatc gatgtcggta ttggtatatc
    24841 cttttgccac tattctattt ctgctttcgc tttatttaat tccaaattgg aataacgcta
    24901 tttttatcca aagcaattcc ttacatgcca atggagggca tggactatta atgactttat
    24961 ggttaattat tccagtgatg gtgttctcct ttaaccattc tcctatcatt tcatcttttg
    25021 ctgtgaatca aaagcagcaa tatggtagcc aagctgacga gaaaagctca ttaattatga
    25081 aatacagtca cttgatgatg gttttctctg tgatgttttt tgtatttagc tgtgtgttga
    25141 gcctgtcacc tcaagatctt ttgcaagcaa agcaacaaaa tatttccatt ttgtcttatc
    25201 tggcgaatca ttttaatacc cctattatgg cctatattgc accggttatt gcattcatag
    25261 ccatttcaaa atcttttctg ggacattatt taggtgccaa ggaaggttta agtggaatta
    25321 ttgttaagtc attaaaatca acaacaggca aaacaattaa taagaattcc ctgcacagaa
    25381 tcattgaaat ctttatgatt ctgacttgtt gggttgttgc tacaattaat cctaatattt
    25441 taaaaatgat tgaaacatta ggaggcccca tcatagctgt cttattattt atcatgccaa
    25501 tgtatgccat tgctaaagtg ccagccatga aaaaatacaa caaccaaatc agtaacgtat
    25561 ttgttaccgt catgggcatt atttctatct cagccatcat ttatggctta atttaacaat
    25621 caatctggta gttgcatata t
//
LOCUS       DBNCWK010000031        24218 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.031, whole genome shotgun sequence.
ACCESSION   DBNCWK010000031 DBNCWK010000000
VERSION     DBNCWK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24218
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.031"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(184..1242)
                     /locus_tag="AC06IO_001165"
     CDS             complement(184..1242)
                     /locus_tag="AC06IO_001165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIO9874366.1"
                     /translation="MTEEVLFSQEGQLGFITLNRPKALNALTLTMIMALQKQLSIWKE
                     DNSIKAVVVQAVPGNAFCAGGDIRWLYNAGRSKDSEQMQFFWHEYRLNHFIHHFGKPY
                     ISLLDGITMGGGVGISLHGSHPVASERFVFAMPETGIGFFPDIGASYLLNKCPGFLGV
                     YLGLTGNKLGPHDARKAGLVKQIVFAEQMQSMIEALKREDLSEDAFNRVDQCISSFAS
                     DAMPTEASQIKPLIDVCFSKPSVELIRESLQSTDGVWALGVDNTLLQKSPLSLKITLA
                     QIQKAKGLSLAECLKMDFDLASHFMKGSDFYEGVRSLLINKDKNPQWRPSSLELVTDA
                     MVVSYFESSSSGLELMIL"
     gene            complement(1245..2021)
                     /locus_tag="AC06IO_001166"
     CDS             complement(1245..2021)
                     /locus_tag="AC06IO_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIO9874367.1"
                     /translation="MNLIEQDIDNNGILTLTLNRPEKLNALSTDVLSALNELFFQAKS
                     NPKVKALLLTGNGKAFCAGADINRLAECNAQTGYEFACQGQEVFRLLETMGKPSLAAV
                     NGFAFGGGCELAISATLRIASNKAMFGQPEVKLGVIPGYGGTQRLARLIGKGRAMDLC
                     LTGRFINAETALNWGLVSEVVAPEDLLTQGKNILNGILSMAPLAVASVMEVIDHGYDL
                     SLTEALHLEAIHFAKVCATEDKKEGVAAFLDKRTADFKGV"
     gene            complement(2038..3192)
                     /locus_tag="AC06IO_001167"
     CDS             complement(2038..3192)
                     /locus_tag="AC06IO_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIO9874368.1"
                     /translation="MHFNFTDEHLAFREMASEFARNKLAPMADFWDEQGYFPIEILRE
                     AAQLGMAGMVVREDIGGAQLSRLDAALIFEQLATGCIPTSAYLSIHNMVASLVDRYGS
                     QEIRKKWGPKLTSMEVIASYCLTEPESGSDAASLKTRAVKEGEYYVLNGAKAFISGGS
                     VSDVYLCMVRTGDESHHGISCLLIEKNTPGLTFGKLEKKLGWRNQPTCMLYFENCRVP
                     IANRVGEEGMGFKIALNALNGGRVNIASCSLGGALACLRMSQSYMHERKQFGKPLTQM
                     QALRFYFADMLTDYEAARLMVYRAAAAMDNDDPNAPMYCAMGKRLATDVAFRISDKAM
                     QLHGGYGYLRDYQIERIFRDLRVHQILEGTNEIMREIIAKASLDEEYFIE"
     gene            3306..5132
                     /gene="recQ"
                     /locus_tag="AC06IO_001168"
     CDS             3306..5132
                     /gene="recQ"
                     /locus_tag="AC06IO_001168"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIO9874369.1"
                     /translation="MGTLAQPSFINPNALAVLKKYFGFDSFRPPQEKIIEDVISGEDV
                     LVLMPTGGGKSLCYQIPAIVRRGVGIVVSPLIALMEDQVTALKLQGIRAAYYNSSLTA
                     EEARNVLNQLHHNELDLLYIAPERLISTSFLDRLSECTISLFAIDEAHCISQWGHDFR
                     PEYACLGLLKTNFPDIPMIALTATADKQTREDIVTKLNYQPKKYVVSFNRPNIHYKVV
                     PKTNAVKQLNLFLQSDTQQSGIIYCGTRHTVEHLTAKLQELGLKARAYHAGLSHTERR
                     EVQNLFRYDRIDIVVATIAFGMGIDKPNVRFVVHHDLPKSIEGYYQETGRAGRDGLPA
                     KALLLYDAADSARLRSWIMNIPLDEQRHIETNKLNHMLAFAEASHCRRQILLRYFDEP
                     SHTECKNCDVCDNPPQTADATEDAQKFLSCIYRLKQSYGLTHTIDVLRGSLSEKIKQS
                     GHEHLSTFGIGKEKSANYWKHLAWQLIHKDYCLQDMNHFNVLRLTPKAIPLLKGEESI
                     SLTIPNNDLNSSKKKGKQSPSFKPESNPLFEKLRALRRQLADEENKPPFMIFSDATLH
                     EMAQLKPKNSKQLLNVSGVGQHKLARYGHYFLEALNEFSEVE"
     gene            5142..5693
                     /locus_tag="AC06IO_001169"
     CDS             5142..5693
                     /locus_tag="AC06IO_001169"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIO9874370.1"
                     /translation="MRKLPRPFYERDTILVAKELLGKYLVHHDGLEEKIGRIVEVEAY
                     LGQHDLACHSSKGLTKRTKVMFGPAGYAYVYLIYGMYYCMNVVTEKEGIGSAVLIRAL
                     EPIKNIQDRTQGPGLLSKAMRIDSKLNHRDLLSNDFYIAEPNSPTDFTIIEKPRVGVH
                     YAKEWANELLRFYIKDNPYISKT"
     gene            complement(5888..6292)
                     /locus_tag="AC06IO_001170"
     CDS             complement(5888..6292)
                     /locus_tag="AC06IO_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HIO9874371.1"
                     /translation="MKKLLSASVITLMITGCAAIPADPQAERVIASPNPAPKGCKYVG
                     QVVGNQGNFFTGGFTSNRNLEEGAMNDLKNKASKKGANYIQLVTNRAGVTGSMSGSFD
                     RHGGFMSGGSEQTNVTNLGNAYICPPKAIGLE"
     gene            complement(6348..6551)
                     /locus_tag="AC06IO_001171"
     CDS             complement(6348..6551)
                     /locus_tag="AC06IO_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HIO9874372.1"
                     /translation="MTDKVYQIVELIGTSQEGIEKAIDNAIKQVAKTHGKLDWFEVVE
                     TRGFIDDNKVKYYQVHLKVGCNV"
     gene            complement(6541..7227)
                     /locus_tag="AC06IO_001172"
     CDS             complement(6541..7227)
                     /locus_tag="AC06IO_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIO9874373.1"
                     /translation="MNEWWLIILLVVLAVFACTLIIYPLRRNRLVSLLLAPIIFIMVF
                     TGYYFWGDFARWQEYLHRNEAQELAQSMLKSIKSPQELIDKLKAKLDAQPESAKGWYL
                     LGRLYSSQKDNQNASLAFAKAHQLKPEDEQFAVNYAHSLWQLSNQQFNPDILEIFHTL
                     LKNNPRQPDALAMLAMHAYLSHDYEAAINYWQRLLQLAPAQSEEALAIRKAIAKAEKQ
                     INRGGKKSDR"
     gene            complement(7220..7621)
                     /locus_tag="AC06IO_001173"
     CDS             complement(7220..7621)
                     /locus_tag="AC06IO_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIO9874374.1"
                     /translation="MRVFHFCFKVILLLLLVSVAFASSMYPLDSARKEAQFNHLLKDL
                     RCLVCQNQDLADSNAQLAKDLRFQVYELVKEGKSDSEIMDYLTARYGDFILFKPPVKS
                     VTLLLWFGPFLFLLIGFIIFWRTCFKRNSHE"
     gene            complement(7621..8154)
                     /locus_tag="AC06IO_001174"
     CDS             complement(7621..8154)
                     /locus_tag="AC06IO_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIO9874375.1"
                     /translation="MKKIGWRVIPIAIFCLLCFFLWRGLSLDPHHLPSARIGKPLPDF
                     ALPELQNPDSTFSAMNFHNQVVLLNVWASWCDACVEEQVFMLQLAREGVPIYGLNYKD
                     NPEHARAWLAQWGNPYRLIGQDSEGKVAIDLGVYGAPETFVIDKKGIVRYRHVGIMNQ
                     KTWLTDILPLMRNLEKS"
     gene            complement(8151..10103)
                     /locus_tag="AC06IO_001175"
     CDS             complement(8151..10103)
                     /locus_tag="AC06IO_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIO9874376.1"
                     /translation="MIAELGLFFLILALVASLMLALIPLAGLQFNRKDWIDSSRNYVV
                     LQFLFVACAYLCLTACFLMDDFSVIYVLNNSSLLLPWFYKFCAVWGGHEGSMLLWVAI
                     LSFWMILVCFFSSSLDTEMRARVLVILGWLSIGFILFLLMTSNPFLRQFQVLNTQGRD
                     LNPLLQDPGFLFHPPMLYMGYVGFCVAFAFAIAALWAGKVETSWSKWTRPWTLAAWCC
                     LTAGITLGSWWAYRELGWGGWWFWDPVENASFMPWLVGTALLHSLAVTEQRQQFKAWT
                     MLLAIAAFSLSLIGTFLVRSGVLTSVHAFAVDPQRGLYILGFLLFVIGGSLTLFLFRA
                     QTLQVNKHPGLLSRESALLLNNVFLVVIMLTVLMGTVYPLLVEGLGLGKLSVGAPYFN
                     AVFVPLIIPLLLLMGLGVRLKWQNDCWRDVFRKLNRIFLLSLILPVILLFCTSTEIHL
                     TALLGLILAFWVLLSTANAAYHKVRDRGVAGVGQAYWGMVLAHCGVAATVIGIAVSSA
                     YGVQDDVRMSPGKQVSLLDYQIGFINQTPLNGPNYKGTRAEFSIQYQNKTRQIFPEKR
                     VYTIGQMPMTESAIDVTPFRDIYIALGEPLSEDAWSVRIYYKPLVRWIWAGGFMILAG
                     GVLALTDRRYYRKRQVTVMAGQEIKI"
     gene            complement(10100..10531)
                     /gene="ccmE"
                     /locus_tag="AC06IO_001176"
     CDS             complement(10100..10531)
                     /gene="ccmE"
                     /locus_tag="AC06IO_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIO9874377.1"
                     /translation="MTPVRRRKLFILLFALSVLSAAAALVLYALRQNISLFYTPTQIV
                     AGEAPAKHHIRVGGMVEANSIVRAKKGLDVQFKITDFENTIVVTYSGILPDLFREGQG
                     IVAEGEVTDNHHFHAIQVLAKHDANYMPPQVKSALADKVKQ"
     gene            complement(10537..10695)
                     /gene="ccmD"
                     /locus_tag="AC06IO_001177"
     CDS             complement(10537..10695)
                     /gene="ccmD"
                     /locus_tag="AC06IO_001177"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIO9874378.1"
                     /translation="MNQFFEWLAMGGYSLYVWPAYGLVCVVLGMNILGIKWQRTRTRK
                     KLQRWFKR"
     gene            complement(10679..11434)
                     /gene="ccmC"
                     /locus_tag="AC06IO_001178"
     CDS             complement(10679..11434)
                     /gene="ccmC"
                     /locus_tag="AC06IO_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HIO9874379.1"
                     /translation="MWKILYQLASPKNFYNYAGRLIPWLAVSALATIAIGMVWGLVFA
                     PPDYQQGDAYRIIFVHVPSAFLSMALYAWMGFLAILLLVWRIKMAGLLIHKVAQLGAC
                     MAFLALITGSIWGKPMWGAWWVWDARLTSELILLLLYLAILATYQAVKNKEDGDKIIA
                     ILALVGLIDLPIIHYSVYWWNTLHQGATLSVFAKPKIAPSMLYPLLITLLGFFLYSLW
                     IILEKARNEVLFRERKQSWVKVQFEEESDESVF"
     gene            complement(11682..13250)
                     /locus_tag="AC06IO_001179"
     CDS             complement(11682..13250)
                     /locus_tag="AC06IO_001179"
                     /inference="COORDINATES: protein motif:HMM:NF013752.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISLpn13 family transposase"
                     /protein_id="HIO9874380.1"
                     /translation="MIKQENWIYPIELSKKEEKICSKLKQNGKLFIFLRKNRHLIFTA
                     DISQKLLSMYADYPRGKPLISPTLLAMVSILQAYEQLSDSSAVLETMFDQRWQMVLGC
                     LGAEESIFSQGTLCDFRHRLVKHNLDTMLLEQTVEVAKEVGGFCYKQLRIALDSAPLE
                     GAGRVEDTFNLIGHALELVVDCAAQIKQVDEIEIIKEAGTYLIGRSSIKAALDIDWSD
                     IQEKQKAINTLLKDVEALQRWLSGQSDSLINNQYLKEALSLLDTVLNQNIEPDPDGGS
                     KIIRGVSTDRRISISDSDMRHGRKSSSRTINGFKQHIAIDLDHKLILATSVRPANEPE
                     HEASQWLKPKVEKYGEVASKSIDRGYLSASWTAELFSQGKKIIAKPWNPATHGKYGKK
                     DFIIDLQNSMVICPAGKQQKITGKPGSLMAKFSAKLCGACPIKNKCSDSKTGRVIQIH
                     DQEKMLQALKYYVTSPEGRQEARERVKVEHALATICNRKGPRARYIGLRLNEYDLNRT
                     AMITNLHIAMNLAA"
     gene            complement(13336..14016)
                     /gene="ccmB"
                     /locus_tag="AC06IO_001180"
     CDS             complement(13336..14016)
                     /gene="ccmB"
                     /locus_tag="AC06IO_001180"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIO9874381.1"
                     /translation="MNSPFQLFGKQFRREILIQVRQIRYLVNSCLFFLMLLFIFPLTL
                     RPELMLMRMFAPGLVWMAILLSMLLSLERLFQQDYEQGVLEQWLVSGQPLNLLVTAKV
                     MAHWLISVTPLLLLSPLIAILFSFNVWETCILLLSLICGTPAILFLCALAAAFGIGIN
                     QRGALMALILLPLTLPLLIFGSGTLSVAMQGLPVSGYLALLLAMSLLAVGFLPYAISA
                     VIRISHID"
     gene            complement(14013..14615)
                     /gene="ccmA"
                     /locus_tag="AC06IO_001181"
     CDS             complement(14013..14615)
                     /gene="ccmA"
                     /locus_tag="AC06IO_001181"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIO9874382.1"
                     /translation="MLDVIELDFDYHDQPLLQQISFHLPAGGLLHLKGSNGAGKTTLL
                     KLIAGLLNPEKGEILFERRSIKKDLCTYQKQLCFVGHRSGINPYLTLRENCLYDIHFS
                     PGAVGITELCRLFSLEHLIDYPCGLLSSGQKRQVALLRLWMSKAKLWLLDEPLVALDE
                     LSLLTIIRKIQEHRAKGGAVLLTSHQDLPLNKADYEEYHL"
     gene            14763..15698
                     /gene="sohB"
                     /locus_tag="AC06IO_001182"
     CDS             14763..15698
                     /gene="sohB"
                     /locus_tag="AC06IO_001182"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HIO9874383.1"
                     /translation="MEFLSEYGMFLLKCITLVIALLILLAGIFSMGRKTKPKLEITSL
                     NEEYQHLNALMNKEILGKKPGKKKKDKTKRPVLYVIDFSGDIKATQVEQLRDEVTSVL
                     SIAKPEDEVLVRLESPGGAVNGYGLAASQLQRIRDKKIPLTVSIDKMAASGGYLMACV
                     ANKIIAAPFAIIGSIGVVAQIPNFHRWLKKNNIDVELLTAGEYKRTLTLFAENTEKGR
                     KKFQEDLEKIHTAFREYVLKNRSQLDIDKVATGEHWIAKDAFDLRLVDKLATSDEYLI
                     EKMAEFNAFKLTVHAKLPIISKVLKPAMRLIHPWI"
     gene            15921..16202
                     /locus_tag="AC06IO_001183"
     CDS             15921..16202
                     /locus_tag="AC06IO_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874384.1"
                     /translation="MSDFKSKLPDLNELTSMTAKLFKGIKTSVSEIIQDYKQKRAQPV
                     STEEKTTENKPAESKAEPKAESKTTENKTEPKTQSKPESNTEETQEKEK"
     gene            complement(16246..17661)
                     /locus_tag="AC06IO_001184"
     CDS             complement(16246..17661)
                     /locus_tag="AC06IO_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HIO9874385.1"
                     /translation="MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVN
                     PNPSVIVLGACTSFEKMMDELDTLVKKFPKTPILVIDAVLSTSECADRNIVDCLSFPF
                     SYAKMLKSLHRCQIAKEAVKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEAS
                     VLILGESGTGKEVVARNIHSLSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAI
                     TSRQGRFELANGGTLFLDEIGDMPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAA
                     THRNLEIAIQEGKFREDLFYRLNVFPIEMPPLRNRSSDLSLLFNELVSRIEGEGRPTV
                     RLMPDAMDALCEYSWPGNVRELANLVERLSILYPNGIIGKSDLPHRFQGENKPSCPEA
                     SGAERETLLQIIKQESVANAEGIDLKEHLVKTELALISQALDESDWVVAHAASYLNMR
                     RTTLVEKMRKYGLTRPERMER"
     gene            complement(17857..18450)
                     /locus_tag="AC06IO_001185"
     CDS             complement(17857..18450)
                     /locus_tag="AC06IO_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874386.1"
                     /translation="MSNFRNFLVFILLFFSFCGQLTASSKQNLAFRNFWHPTYLGQRL
                     DYCTLDGKECGKEVANRYCQMLGYDYSTQNVIAYNVGLTNYLGSKAQCKGWRCNGFMT
                     IACAIGLSHTPPKPYHYREKKFAVPRYNDYRVDWCMERNKGCGKQAANSFCNRMGYMQ
                     AKDFVKQAQVSATKTIGSQELCFGNQCNAFKMIICYR"
     gene            18839..19855
                     /locus_tag="AC06IO_001186"
     CDS             18839..19855
                     /locus_tag="AC06IO_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9874387.1"
                     /translation="MKKYLTFIVSGLALIAIIWFIRHLWIIETVKVVHPVYGTAVEAV
                     YATGTVEPTVMMPIASQNTARLIKLNADEGSEVVKGQILANLEDEHLQHNLKELKEHE
                     KLAKIRYERNAQLVKKGVISKDEYDRTLSEWQAASAAVRRIEAQISYLQLIAPADGRI
                     IKRDGEVGQLIPANQPVFWLSCCAPLRVSAEVDEEDIVLVRVGQKVLIRSDAFPNQIF
                     YGRVKSITPKGDPIARSYRVRISFSENNPLLIGMTAETNIIVHEKKNALLLPVSSISE
                     NKVWIVQDDGKLSQNTVTIGARGLKQAEILKGVNINDLVVLQPDNKLKNGQKVRFILV
                     KQEE"
     gene            19855..21105
                     /locus_tag="AC06IO_001187"
     CDS             19855..21105
                     /locus_tag="AC06IO_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIO9874388.1"
                     /translation="MKLFLKIALKHLLARKRQSLVSLMGIVLGVAFFLTISSLMQGSE
                     KDFIKRLVDNSPHITIIDEYRNPRIQPIQQLHTHGAIEIRSVKPLTEPRGIRGYEQTI
                     NNLSLTIPGIVISPVLIGQALINYAGKDYSVTLNGMIPEEIKKVSTIENYMTSGSIDD
                     LIVNPDGIVLGSELLRKLSLTKDDNITVTAPNGQIRTFKILGVFRTGRSDFDTNQAFI
                     SIKRAQALLNRPNRSNNIIIKLPNPYQAYDIAAQIEKQIGYKTISWQEKSEDLLNTLV
                     IRNIIMYSVVSAVLIVAAFGIYNVISTVVMEKHRDIAILKSIGFQKHDIQFIFIIEGF
                     LLGLVGCLLGLPLGSGLMYSLMQVQFKPPGSSELINMPLDWSYLQFVIATAFAMSASI
                     IAAALPAHKAALVHPVDILRGGGM"
     gene            21102..21779
                     /locus_tag="AC06IO_001188"
     CDS             21102..21779
                     /locus_tag="AC06IO_001188"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9874389.1"
                     /translation="MNKIVIKTKELSRQLSGEVPVTLVKDINLEVREGEFLVISGPSG
                     SGKSSLLYLLGLLDRPSQGTIWLNNEDVSTYEEEKMAWLRLTQIGYVFQFHFLLPEFT
                     ALENVMLPMQRLGKLSLGEMKNRATKLLTSLGLEEQLSKLPKQLSGGQSQRVAIARAL
                     ANDPILLLADEPTGNLDTVSSTNVQNILKELAHEYRRSVIIVTHDMNFAATADRVIHI
                     VDGKMQE"
     gene            complement(21965..22723)
                     /locus_tag="AC06IO_001189"
     CDS             complement(21965..22723)
                     /locus_tag="AC06IO_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIO9874390.1"
                     /translation="MITRDELSLFLLDFLNCSQYQDYAPNGIQVEGKDKIKRICTAVS
                     ASEDVISQAIEQQADALLVHHGYFWKGENPVISGMKRRRIAKLLGHNMNLFAYHLPLD
                     CHPELGNNASLANLLLIESHEMHKVNNTANLLWSGKLSKAMNSEQFSSFLEHKLGRYP
                     VHIAGNEKMIHSIAWCTGAAQDFIEEAYQLGVDAYLSGEVSERTFYQAMELGIQYFSC
                     GHHATERFGIQSLGVYLANYFDLEHWFIDSPNPI"
     gene            complement(22720..23988)
                     /gene="murA"
                     /locus_tag="AC06IO_001190"
     CDS             complement(22720..23988)
                     /gene="murA"
                     /locus_tag="AC06IO_001190"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIO9874391.1"
                     /translation="MDKLLINGGKALHGEVVISGAKNAALPIMAASLLASDHVTISNV
                     PHLKDITTMMELLGQLGAHLIVDEKMNVQVDSSQVNEFVAPYDLVKTMRASILVLGPM
                     LARFGKADVSLPGGCAIGTRPVDLHLKALRAMGADITVKNGYINARCKKGRLQGKRLM
                     FDTVTVTGTENVLMAAVLAEGITTIKNAAREPEVVDLANFLIQMGAKIRGAGTSTIEV
                     EGVESLNGGTYSVMSDRIEAGTYLAAGALTRGQVTVKKVRPDTLLSQLCKFEEAGAEL
                     TIGEDWVSLNMHNKRPQAVNISTAPYPAFATDMQAQFMAMNSVAEGSSTIIETIFENR
                     FMHVQELQRMGANIQLNGNTAIVHGVEKLTGAPVMATDLRASASLILAGLVAEGETVV
                     ERIYHVDRGYERIEEKLSLLGADIKRVSDR"
     gene            complement(23981..>24218)
                     /locus_tag="AC06IO_001191"
     CDS             complement(23981..>24218)
                     /locus_tag="AC06IO_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIO9874392.1"
                     /translation="NEEIEQRLNEIDDVFYVKVEGDGYQYQVTVVTDIFLKKSKVARQ
                     QWVYAQLKDFITTGRLHAISMKTWTKEEWGKQNG"
BASE COUNT         7721 a         5279 c         4269 g         6949 t
ORIGIN      
        1 ttacaaccgc aagcttgaag ctcttttgac gaaggagaca tttttgggat gagcacattt
       61 tattaaatta ttacttttag atacttcctt atgacagcaa gcagataata tttcagactg
      121 ctttcaacca taaggaagta ttcatacggg aaatctatta ttcccccaat caaaatccaa
      181 aaactaaagt atcattaatt ccaatccgga agatgagctt tcaaaataac tcactaccat
      241 ggcatcagtg accaactcca agctggacgg tctccattga ggatttttat ctttattaat
      301 caataaagag cgaactcctt cataaaaatc actacctttc ataaaatgac tggcgagatc
      361 gaaatccatt tttaaacact ctgctaaaga taagcccttg gctttttgga tttgtgcaag
      421 ggttatctta aggctcagag gcgatttttg cagcaaagta ttatccaccc caagagccca
      481 cacaccgtct gtgctctgta aagactctct tattagttca acactaggct tggagaaaca
      541 gacatcgatt aaaggtttga tttgacttgc ctcagtgggc atggcatcac ttgcaaaaga
      601 agaaatgcat tgatcaacac gattgaaagc atcttctgat aaatcttctc tctttaatgc
      661 ctcaatcatt gactgcattt gctctgcaaa gacaatctgc ttcactaacc ctgccttcct
      721 ggcatcatga ggacccaatt tatttccggt taaccccaaa tagactccta aaaaccctgg
      781 gcatttgttt aataaatagc tggcaccaat atcaggaaaa aaaccaatcc ctgtttctgg
      841 cattgcaaat acaaaacgct cactggcaac agggtggctt ccatgtaagg aaataccgac
      901 ccctcctccc atagtaatcc catccagcaa agaaatataa ggtttaccaa aatgatgaat
      961 aaaatggttt aacctgtact catgccaaaa gaattgcatc tgctcagagt ctttactacg
     1021 gcccgcatta tatagccaac gaatatcacc acctgcgcaa aaagcattac ccggaacagc
     1081 ttgtacgacg actgctttaa tagaattatc ttctttccaa attgacaatt gcttttgcag
     1141 tgccatgatc atggttaaag ttaaagcatt taaagccttt ggtcgattta aagtaatgaa
     1201 tcccaattga ccttcctgag aaaacagcac ctcttcagtc ataattaaac ccctttaaaa
     1261 tccgcggttc tcttgtctag aaacgcagct actccttcct tcttatcttc agtggcacaa
     1321 acttttgcaa aatgaattgc ttccaaatga agtgcctctg tgagtgataa atcataacca
     1381 tgatctatca cttccattac actggcaact gctagaggag ccatgcttaa tattccgttt
     1441 agaatatttt ttccttgagt caacaaatcc tctggtgcta caacttcact gactaacccc
     1501 cagtttaagg cagtctccgc atttataaag cgcccagtca aacataaatc catcgcgcgc
     1561 cctttgccaa ttagcctggc caatcgttgt gtgccgccat acccgggaat aacacctaat
     1621 tttacctcag gctgaccaaa cattgcctta tttgaagcaa ttctaagagt cgcagatatc
     1681 gctaactcac atccgccacc gaaagcaaaa ccattcactg cggcaagtga aggtttcccc
     1741 atcgtttcca aaagtcgaaa aacctcttgt ccctggcaag caaattcata accagtttga
     1801 gcgttacatt cagccagtcg attaatgtca gccccagcgc aaaatgcttt accatttcct
     1861 gttaacaata atgctttgac tttgggattg gatttcgctt gaaagaataa ttcatttaat
     1921 gcgctcagaa catcagtgct taaagcattg agcttctctg gcctattcaa ggttaaagtt
     1981 aagattccgt tattatctat atcctgttca attaaattca tcaataatcc cctatgatta
     2041 ctctataaaa tactcttcat ccaaacttgc tttcgctata atttcacgca tgatttcatt
     2101 agtgccttca agaatttgat gaactcttaa atctctaaat attctttcaa tttgataatc
     2161 tcgcaggtag ccataacctc catgcaattg catggcttta tcactaatac gaaatgcgac
     2221 atctgtagcc aagcgcttgc ccatagcaca atacattggc gcattgggat catcattatc
     2281 cattgctgct gcagccctat aaaccatcag ccttgccgct tcataatctg ttaacatatc
     2341 cgcaaaataa aaacgaagag cttgcatctg agttaaaggt tttccaaact gtttgcgctc
     2401 atgcatataa gattggctca ttcgcaggca agcaagggcc cctcctaatg agcaggaagc
     2461 aatattcact ctccctccgt ttaaggcatt cagtgcaatt ttaaaaccca taccttcttc
     2521 accaacacga ttggcaatgg gcacacgaca attttcaaaa tatagcatac aagttggctg
     2581 atttcgccaa cctaattttt tctccagttt gccaaatgtc agaccaggag tattcttttc
     2641 aattaacaaa caactgatgc catgatgaga ctcatcacct gtacgtacca tacataaata
     2701 cacatcacta acacttccac ccgatatgaa tgctttcgca ccattaagaa cataatattc
     2761 accctctttt accgcgcgag tttttaaaga ggctgcatca gatcctgatt ctggctctgt
     2821 aagacaataa ctggctatca cttccatact ggttaatttt ggtccccatt tcttgcgaat
     2881 ctcttggctg ccatagcggt cgaccaatga agcgaccata ttatggatgg agagatatgc
     2941 acttgttgga atacagcccg tagcgagttg ttcaaagatg agtgctgcat ccagacgaga
     3001 gagctgagca cctcctatgt cttcgcggac taccattccc gccataccta attgcgccgc
     3061 ttctcttaaa atttcgattg gaaagtaacc ttgttcatcc cagaaatctg ccattggagc
     3121 cagtttgtta cgagcaaact cggaggccat ttctcgaaat gccaaatgtt catcagtgaa
     3181 attaaaatgc atagaccatc cttagattta ttgattttag tcattggatt tatttatgac
     3241 gctttcttaa aaaatgatat tattgcgaat ttttagcgcc caggaaagat aataagaata
     3301 attacatggg aactttagcg caaccatctt ttataaatcc aaacgcatta gctgttttaa
     3361 aaaaatattt tggatttgat tccttcagac ctcctcagga aaaaataatt gaggatgtca
     3421 tttctgggga agatgtactg gttttgatgc ctactggagg gggaaaatcg ctttgctatc
     3481 aaattccagc cattgttcgc cgtggtgttg gcattgtagt atctccttta atcgcgttaa
     3541 tggaagatca agtaaccgct ctcaaactgc agggtattcg tgcggcttac tacaactcat
     3601 ccttaaccgc cgaagaggct agaaatgtat taaatcaatt acatcataat gaattggatt
     3661 tactctatat tgctccagaa cgtcttatta gtacctcgtt tctggatcga ctgtcagaat
     3721 gtactatctc tttatttgct atcgatgaag cacactgtat ttcacaatgg ggacatgatt
     3781 ttcgacctga atacgcctgt ttaggtttac tgaaaactaa ctttcctgac attcctatga
     3841 ttgctctgac agctactgcc gataagcaaa cacgtgagga tattgttacc aagttgaatt
     3901 atcaacccaa aaaatacgtt gtttcattca atcgaccgaa tatccattac aaagtagttc
     3961 ctaaaacaaa tgcagtgaag caattaaatc tgtttttaca atctgatacc cagcaatccg
     4021 gaattattta ttgtggaacc cgccatacag tcgaacatct tacagcgaaa ttacaagaac
     4081 ttggattgaa agcacgcgct tatcatgcag gcctgtctca tacggagcgg cgtgaggtgc
     4141 aaaacctttt cagatacgat cgcattgata ttgtggtagc tacaattgct tttggaatgg
     4201 gtattgacaa gcctaatgtt cgctttgttg ttcaccatga tttacccaaa agcatcgaag
     4261 gttactacca ggaaacaggt cgtgcaggac gtgacggatt accggccaag gcccttcttc
     4321 tttatgatgc tgcggatagt gcccgtttgc ggtcctggat aatgaacatc cctcttgatg
     4381 agcaaagaca tatagaaacc aataaattaa atcatatgct tgcttttgct gaagcgtcgc
     4441 attgtcgtcg tcaaatttta ttacgctatt ttgatgaacc ttctcataca gaatgtaaaa
     4501 actgtgatgt ctgcgataac ccaccgcaaa cagccgatgc aacagaagat gcgcaaaaat
     4561 ttttatcgtg tatttatcga ttaaaacaaa gctatggttt aactcatact attgatgttc
     4621 tgagaggtag tttatctgaa aaaatcaagc agtctgggca tgagcattta agtacctttg
     4681 gaattgggaa agagaaatct gccaattatt ggaagcatct tgcctggcaa ttaattcata
     4741 aagattattg tttacaggac atgaatcatt ttaatgtgtt acgcttgacc ccaaaagcga
     4801 ttcccctcct aaaaggtgaa gagagtattt cattaaccat ccctaacaat gatctgaata
     4861 gcagtaaaaa gaaaggcaag cagagtcctt cctttaaacc tgaaagtaac ccattatttg
     4921 aaaaacttcg tgcgttaaga cgtcagttgg ccgatgaaga aaacaaacca ccatttatga
     4981 tattcagtga tgctacatta catgaaatgg cccaattaaa acccaaaaac agtaagcaac
     5041 tactgaatgt atctggggtt gggcaacata aattagctcg ttatggccat tattttctcg
     5101 aagccttaaa cgaattttca gaagtcgagt gagattccgt tatgcgtaaa ttaccaagac
     5161 ccttttacga acgtgatacc attctggttg caaaagaact cttaggtaaa tatttggttc
     5221 atcatgatgg attggaagag aagattgggc gcattgtaga agtcgaagcc taccttgggc
     5281 aacatgattt ggcctgccat tcatcaaaag gattgactaa aagaactaaa gttatgtttg
     5341 ggccagcagg ctatgcgtat gtttatctaa tttatggcat gtattattgc atgaacgtag
     5401 tcaccgaaaa ggaggggata ggttcagcag tattaatacg tgccctggag cctatcaaaa
     5461 acattcaaga cagaactcaa ggaccagggt tattatccaa agctatgcgc attgactcca
     5521 aactgaatca tcgtgattta ttaagcaatg atttttatat tgctgagcca aacagtccaa
     5581 ctgattttac cattatagaa aaacccagag ttggggttca ttatgctaaa gaatgggcta
     5641 atgagttatt aagattttat attaaagaca atccttatat ttcgaaaacc tgattaggcc
     5701 tcgtgcataa actgtctctt tcattttatt gccgcattgc aaagatttct gtgatgctca
     5761 gggtatgtaa aaagcgtatt atcgtattaa tgagcccatt attattacat ccctttcttc
     5821 aagcatgtcc tgtcaatccc cggatccagg ctttgtgcct aatccggggt aatttcttga
     5881 aataactcta ctctaaccca attgccttgg gcgggcagat ataggcatta cccagattag
     5941 tcacattggt ttgttctgaa ccgccgctca taaaaccacc gtgacgatca aatgaaccac
     6001 tcatggaacc cgttacaccg gctcggttcg tcaccaattg aatgtaattt gccccttttt
     6061 tgcttgcttt attcttcaga tcattcattg ctccttcttc caaattgcga ttggaagtaa
     6121 agcctccggt aaagaaatta ccctgattgc ctacaacctg gcccacgtat ttgcaacctt
     6181 taggtgccgg attcgggctt gcaatgacac gttcggcctg aggatctgct ggaatagctg
     6241 cgcatccggt aatcatcaaa gtaataacag acgcagatag tagttttttc ataattacac
     6301 ctttttgtta tcaacaaaac aatgaaatgt taacaactcc tgtttgatta aacattacaa
     6361 cccactttta aatgcacttg ataatatttc actttattat catcaatgaa acctcttgtt
     6421 tctactactt caaaccaatc caattttcca tgcgttttag ctacttgctt tattgcatta
     6481 tcaatcgctt tttcaattcc ttcttgcgaa gtcccaatta actccacgat ttggtaaact
     6541 ttatctgtca ctcttctttc cccctctatt aatttgtttc tcagcttttg ctattgcctt
     6601 gcgaatagcc aatgcttctt cggactgcgc tggcgccaac tggagtagcc gttgccagta
     6661 attaatcgca gcttcataat catgacttaa gtaagcatgc atcgcaagca tggctaaagc
     6721 atccggttgc ctggggttat tttttaataa ggtatgaaaa atttctaaaa tatcaggatt
     6781 gaactgttga ttactcaatt gccataaact gtgggcataa ttgactgcaa attgctcgtc
     6841 ttcaggttta agctggtggg cttttgcaaa agcgagggaa gcattttggt tatccttttg
     6901 actgctgtat aatctgccta acaaatacca acccttggca ctttcaggct gcgcatccaa
     6961 cttggccttc agcttgtcga ttaattcctg agggctttta atcgatttta gcatcgattg
     7021 cgctagctcc tgagcctcat ttcgatgtag atattcttgc caccgagcaa aatctcccca
     7081 aaaatagtac cctgtaaaaa ccattataaa aataatgggg gcgagcaaca aacttaccaa
     7141 acgattacgc cgcaaaggat agataataag agtgcatgca aaaacggcta aaacaaccaa
     7201 taaaataatt aaccaccatt cattcatgag aatttcgctt aaaacatgtc cgccaaaaaa
     7261 taataaagcc aatcaataaa aacaggaatg ggccaaacca caataaaaga gtcacgctct
     7321 taacaggcgg tttaaatagt ataaaatccc cataacgtgc ggttagataa tccattattt
     7381 cactatcgct tttgccctct ttcactaatt cataaacctg aaaacgtaaa tccttcgcta
     7441 attgagcatt tgaatctgcc aaatcctgat tttggcatac caaacaacgc aaatctttta
     7501 aaagatggtt aaattgtgct tcttttcttg ctgagtccaa tggatacata ctacttgcaa
     7561 acgcaacgga tactaatagc agcaataaaa tcaccttaaa acagaagtgg aatactctca
     7621 ttatgatttc tccaaattcc tcattaaagg taaaatatca gttaaccagg ttttctgatt
     7681 cattatcccg acatgcctgt agcggactat tcctttctta tcaatgacaa aagtttcagg
     7741 cgccccatat acacctaagt caatagcgac tttaccttct gaatcctggc ctatgagtcg
     7801 ataagggttc ccccattgtg caagccatgc tctcgcgtgc tctggattgt ctttataatt
     7861 taaaccatag ataggtacgc cttctcttgc gagttgcaac ataaatactt gctcttcaac
     7921 gcaagcatca caccaacttg cccagacatt aagcaaaacg acctgattat gaaagttcat
     7981 tgcactaaaa gtagaatcgg gattttgcaa ctctggtaag gcaaagtctg gtaatggctt
     8041 tccaattcga gctgaaggca aatgatgagg atccaaagat aaaccccgcc agagaaaaaa
     8101 acataaaaga caaaaaatag cgataggaat aacacgccac cctatttttt tcatatcttg
     8161 atctcctgcc ctgccattac cgttacttgc ctttttctgt aataccttct atctgttaat
     8221 gccaaaacgc ctccggctaa aatcataaat ccgccagccc atatccagcg gaccaacggt
     8281 ttataataaa ttcggaccga ccaggcatcc tcagacagtg gctcccccaa agcaatgtaa
     8341 atatctcgga aaggagtcac atcaatcgca gactcagtca ttggcatttg tccaatggta
     8401 tacactcttt tttcaggaaa aatttgccta gttttattct gatattgtat tgaaaactca
     8461 gcccttgtcc ctttgtaatt tgggccattc aacggagtct gattaatgaa tccaatctga
     8521 taatccagca gactcacttg cttgccagga gacatacgta catcatcttg tacgccataa
     8581 gctgaagaaa cagcaatgcc aatcactgtt gctgcgacac cacaatgagc caaaaccatc
     8641 ccccaataag cctgtccaac tcctgccacc cctctatctc tgactttgtg atacgccgca
     8701 ttagcagtac tcaacaaaac ccagaatgcc aaaattaatc ccaaaagggc tgtaaggtgt
     8761 atttcagttg aggtacagaa tagtaaaatg acaggcaata tcagactaag caaaaaaata
     8821 cgatttagtt ttctgaagac atccctccaa caatcattct gccatttcaa acggacacct
     8881 aaccccatta gcaataataa aggaattatt aatggaacaa atactgcatt aaaatagggc
     8941 gctccaacgg ataatttgcc taaccctaac ccttccacca gcaatggata aaccgtgccc
     9001 attaataccg tcagcataat aaccactaaa aacacattgt ttaacaacaa cgcactttct
     9061 cgggataata agccaggatg tttattgact tgcaaggttt gggctctgaa aagaaacaat
     9121 gttaaagaac ccccaatgac gaataacaaa aatcccagaa tatataatcc ccgctgagga
     9181 tcaaccgcaa aggcatgaac tgaagttaaa actccagagc gaacaagaaa agtaccaatt
     9241 aaacttaaag aaaaagcagc gattgcaagc aacatggtcc acgctttaaa ttgctggcgc
     9301 tgctctgtaa ctgccaaaga atgcaatagt gccgtaccaa ccagccaggg cataaaagaa
     9361 gcgttttcta caggatccca aaaccaccaa ccaccccagc ctaattctct ataagcccac
     9421 caactgccca aagtaattcc cgcggttaaa caacaccagg cagccagagt ccagggtctg
     9481 gtccatttag accaactggt ttctactttc cctgcccaca aggcggcaat agcaaaagca
     9541 aaagcgacac aaaaccctac atatcccatg tacaacatag gaggatggaa taaaaagcca
     9601 ggatcttgca gcaatggatt cagatcacgg ccctgtgtat tcaatacttg aaattggcgc
     9661 aagaaaggat tggaagtcat taacaaaaaa agaataaaac caatgcttag ccaacctaaa
     9721 ataactaata cccgagcgcg catttccgta tcaaggcttg agctaaaaaa acaaaccagg
     9781 atcatccaaa aactaaggat agcaacccat aacagcatgg aaccttcatg cccaccccac
     9841 acagcacaaa atttataaaa ccatgggagc aataagctgg agttattcag cacatagatc
     9901 accgaaaaat catccatcaa aaaacaggcc gtgagacata ggtaagcaca tgccacaaat
     9961 aaaaattgca gcacgacata attcctggag gaatcaatcc agtctttccg attgaattgc
    10021 aaaccagcta atgggatcaa agctaacatc aaagaagcca ctaaagccaa aatcaaaaaa
    10081 aacagtccta attccgctat cattgtttca ccttatcagc taaagcagac tttacttgag
    10141 gtggcatata attggcatcg tgttttgcca aaacttgtat agcatgaaaa tgatgattat
    10201 cagttacttc tccttctgca actattcctt gcccttctcg aaataaatcg ggaagtatcc
    10261 ctgaataggt aacaacaatc gtgttttcaa aatctgtaat tttaaactgg acatccaaac
    10321 cttttttggc acgtactatg ctgtttgctt ccaccatacc tccaactcgg atgtggtgtt
    10381 ttgctggtgc ttctcccgca acaatctgcg tgggggtgta aaataaactg atattttgtc
    10441 ttaaggcata tagaaccaag gcagccgctg cagataaaac agacagggca aacagtaaga
    10501 taaataattt gcgtcgccgt acaggggtca tttcacctat ctcttaaacc aacgttgtaa
    10561 ttttttgcgg gtacgagttc tttgccactt tattcctaaa atattcatgc ccaaaaccac
    10621 gcaaaccaaa ccataagcag gccaaacata gagagaataa ccgcccattg ccaaccattc
    10681 aaaaaactga ttcatcagac tcttcctcaa attgaacctt aacccatgat tgctttctct
    10741 ccctaaataa gacttcatta cgtgcttttt ccaaaatgat ccataaggaa tacaagaaaa
    10801 aacccagcaa agtgattaac aaaggataca acatactggg agcaattttg ggtttggcaa
    10861 acacagataa agtcgcacct tggtgtaaag tattccacca ataaactgaa taatgaatta
    10921 ttggtaaatc aattaagccc accaaagcta aaattgctat tattttatct ccatcttctt
    10981 tattttttac cgcttggtag gtagccagaa ttgccagata gagtaacaaa agtattaact
    11041 ctgaggtcag gcgcgcatcc catacccacc aggcacccca catgggttta ccccaaatgc
    11101 tccctgtaat taaagcaaga aatgccatgc aggcacctaa ttgcgcgact ttatgaatca
    11161 aaagccctgc cattttgata cgccacacca acaataaaat ggccagaaac cccatccagg
    11221 catacaatgc cattgataaa aaagcgctgg gcacatgaac aaaaataatt cggtatgcat
    11281 ccccttgctg ataatctggt ggagcaaata ccaatcccca aaccatacca atggctatgg
    11341 tagctaaagc actgactgcc agccagggaa tgagacgtcc cgcgtagtta taaaaatttt
    11401 ttggcgatgc caactgatac aatatcttcc acataggaaa caataaaatc aaaaagaagg
    11461 attctaccac gcatcaaaac aatcacaaaa caatcacaaa atttagttcc aaacttaggg
    11521 tctgttgaca tttcgctcgc ttgagtcgaa aaaccttgat tttcgagcac cgtagcggag
    11581 catacataga agtatgtgag taacggagca cagaaaatca aggctttttg gccaagatag
    11641 tagaaatgtc aacagaccct agagcgccga atgatttaaa tttacgcagc taaattcata
    11701 gcgatgtgta aattagtaat catggcagtt cggttcaaat catactcatt aagtcttaat
    11761 ccgatatatc ttgctcttgg tcctttacga ttacaaatgg tcgcaagagc atgctctact
    11821 ttaacacgtt ctcttgcttc ctgacgaccc tcaggcgacg taacataata tttcaatgcc
    11881 tgcagcattt tctcttggtc atgaatttga atcactcttc cagtctttga gtcactgcat
    11941 ttgttcttta tgggacaagc accacagagt ttggctgaga atttggccat caggcttcct
    12001 ggtttgcctg tgattttttg ctgtttgcca gcaggacaaa tgaccattga attttgtaaa
    12061 tcaatgataa aatctttttt cccatatttt ccatgtgttg ctggattcca tggcttagca
    12121 atgatctttt taccttgtga aaataactca gcagtccatg atgcagaaag gtacccccga
    12181 tcaatactct ttgaggcaac ctccccatat ttttcaactt ttggcttaag ccattggctt
    12241 gcttcatgtt ctggttcatt agctggacgt acgcttgttg ccaaaattaa cttatgatca
    12301 agatcaatag cgatatgctg tttaaatcca ttgattgtac gagagcttga tttacgacca
    12361 tgacgcatat cactatctga gatagagatt cttctatctg tagaaacgcc tctgataatt
    12421 ttggaaccac catctggatc aggttcaata ttctgattta aaacagtatc taataatgac
    12481 aatgcttctt ttagatattg attgtttatc aatgaatcag attgcccgct tagccagcgt
    12541 tgtaatgctt caacatcttt aagcaatgta ttaatggctt tttgtttttc ttgaatatcc
    12601 gaccagtcaa tatccaaggc agcctttata ctactacggc caattaagta tgtgcccgct
    12661 tccttgataa tctcaatttc atcaacttgc tttatttgag cagcacaatc aaccactaac
    12721 tctaaggcat gaccaatcaa attgaaggta tcttctactc gaccagcgcc ttctaatggc
    12781 gccgaatcta gcgctattct taactgctta taacaaaatc caccaacttc cttggctacc
    12841 tctaccgttt gctcaagcag cattgtatcc aaattatgtt ttactaatcg atgcctaaaa
    12901 tcacacaatg taccttgaga aaagatgctt tcttctgctc caagacagcc taaaaccatt
    12961 tgccagcgtt gatcaaacat cgtttcaaga acagctgaag aatccgataa ttgctcataa
    13021 gcttgtaata tcgaaaccat tgcaagaagc gtagggctaa tcaatggctt accacgagga
    13081 taatctgcat acatcgacaa taatttctgg cttatatcag cagtaaaaat aagatgtcga
    13141 ttttttctta aaaaaataaa taacttccca ttttgtttta atttgctgca gatcttctct
    13201 tctttttttg atagctcaat ggggtaaatc cagttttcct gctttatcat gcacactcct
    13261 ttacacttgt ctgcatgaat ttgatcataa aatttgaaca atgtcgatct ggttaaaaat
    13321 taatcgatcg gcgctctagt ctatatgact aatacgtatc actgccgaaa tcgcataggg
    13381 tagaaaacca actgccaata gagacatcgc caataataaa gccagatagc cgctgaccgg
    13441 caaaccttgc atcgcaacgc tcaaagtacc actaccaaat atcagtaaag gtaaagttaa
    13501 cggcaataga atcaatgcca tcaaagcccc gcgctgattt atccctatac caaaagctgc
    13561 tgcaagcgca cataaaaata atatggcagg cgtcccgcaa ataaggctca ataacaatat
    13621 acatgtttcc caaacattga atgaaaataa tattgcaatc aaaggagata ataataatag
    13681 cggtgtcaca gatatcaacc agtgtgccat aactttggct gtcacaagga gatttaatgg
    13741 ttgccctgaa actagccact gttcaagaac cccttgttca taatcttgtt gaaacaaccg
    13801 ttctaacgaa agaagcatgg ataataaaat ggccatccac actaatccgg gagcaaacat
    13861 acgcatcaac atcaattctg gtcttagagt aagaggaaaa ataaataaca acattaaaaa
    13921 aaataaacac gaattaacta aatatctgat ttgccgcacc tggattaata tttcacgcct
    13981 gaactgtttt ccgaataatt gaaatgggga attcatagat gatattcctc ataatctgcc
    14041 ttgtttaaag gcaaatcctg atgagaagtc aataatacag caccaccttt tgccctgtgc
    14101 tcctggattt ttctgataat cgtaagcaat gataactcat caagagcaac caaaggttca
    14161 tcaagcaacc ataattttgc ttttgacatc cataatctta ataaagcaac ttgccttttt
    14221 tgtcctgagg atagtaatcc acatggataa tcaataaggt gttctaaact gaataatctg
    14281 cataattctg taatgcccac tgcaccagga gaaaaatgta tgtcatacaa acaattttct
    14341 cttagcgtaa gatagggatt gatccctgat ctatgcccca caaaacataa ttgcttttga
    14401 taggtgcata gatctttttt tatagatcgc ctttcgaaga gaatttctcc cttttcagga
    14461 ttaagcaaac ctgctattaa tttaagtaaa gtcgtttttc ctgctccatt ggaacctttt
    14521 aaatgcaata agcctccagc aggaagatga aaagaaattt gttgcaacaa tggctggtca
    14581 tgatagtcaa aatcaagttc aatcacatca agcataaaac actttatttt tagatacact
    14641 cggctaacta taacaggaca taagaaaaat ccccaatgcc ttcgttaaaa gttatttaat
    14701 ttttgcttgc gaagtgagtg gatgatcgcc ataatctgct ttattttttg caaaggtata
    14761 tgatggaatt ccttagtgaa tatggcatgt ttctcttaaa atgcatcact ctcgtcatcg
    14821 cattactcat tcttctggca ggtatatttt ctatgggtcg caaaaccaaa cccaaactgg
    14881 aaattacctc tcttaatgaa gaatatcagc acctgaatgc cttaatgaat aaagagattt
    14941 taggaaaaaa acctggaaaa aagaaaaaag ataaaaccaa gcgcccggtt ctttatgtca
    15001 ttgatttcag cggtgatatc aaagcaaccc aagttgaaca actaagagat gaagtcacat
    15061 cggtattaag tatagcaaaa cctgaggatg aggttttagt aaggttggaa agccctggag
    15121 gggccgtcaa tggttatgga cttgctgcgt cccagttaca gcgaatccgt gataaaaaaa
    15181 ttcctttgac tgtcagcata gacaaaatgg cagccagcgg cggatattta atggcctgcg
    15241 tagcaaataa aataattgct gccccatttg caataatcgg ctctataggc gtagttgctc
    15301 aaattcctaa ttttcatcgc tggttaaaga aaaacaacat tgatgttgag ctattaactg
    15361 caggagaata caaacgcacc ctaacacttt ttgcggaaaa tactgaaaaa ggtagaaaaa
    15421 agtttcagga agatttggaa aaaattcata ctgcattcag agagtatgtt cttaaaaata
    15481 gaagtcaact cgatatagat aaggttgcaa ctggtgaaca ttggatagca aaagatgctt
    15541 ttgatctccg gctcgttgat aagttagcta ctagcgatga atatttaata gaaaaaatgg
    15601 cggagtttaa tgcattcaaa ttaactgtcc atgccaaatt gccaattata agcaaggtgc
    15661 ttaaaccggc aatgagactc attcatccgt ggatttaaaa attagtactg aaacagcttg
    15721 ggaacgccat tgaaattcaa taccgttaaa tcaggcagca ttccatagga aacgagatca
    15781 attttatcat gggcttaaag ctgatgatgg tagcggaatt taaggaatta atgagcaaaa
    15841 attttcaaaa tccgccatta accaaaattt gaatctcggt atataattaa agaataacaa
    15901 ttttctctga ggagtaagac atgagtgatt tcaaatcgaa gttaccagat cttaatgaat
    15961 taacatcaat gaccgcgaaa ttatttaaag ggattaaaac cagtgtcagt gaaataattc
    16021 aggactataa gcaaaaaaga gcacagcctg ttagtactga agagaaaacc acagaaaata
    16081 agccagctga aagcaaggcc gaaccaaaag cagaaagcaa gactactgaa aacaaaactg
    16141 agccaaaaac acaaagcaaa cctgaatcaa atacagaaga aacacaggaa aaagagaaat
    16201 aatcctgtgc tagcaaataa gtgatacggg gtgagctgta tctatctatc gttccattct
    16261 ttctggtcga gttaagccat atttgcgcat tttttcaact aaagttgtcc ttctcatgtt
    16321 gagataactg gccgcatgag caacaaccca atctgactca tctaaggcct ggctaatcaa
    16381 ggcaagttct gttttaacca aatgctcttt taaatcaatg ccctctgcat tagctactga
    16441 ctcctgcttt attatttgca acaaagtttc acgttccgcc cccgatgcct ctggacaaga
    16501 tggtttattt tcaccttgaa accgatgagg taaatcactc ttgccaataa taccattagg
    16561 gtaaagaata gataatctct ccaccaaatt tgctaactcc ctcacgttac ctggccagct
    16621 gtattcgcat aaagcatcca tagcatcggg cattaatcga acggtaggtc tgccctctcc
    16681 ttcaattcga gaaaccagct cattaaaaag cagtgataaa tcactacttc tattacgtag
    16741 tggaggcatt tcaatgggaa atacattcaa tctgtaaaac aaatcttctc taaatttccc
    16801 ttcttgaata gcaatttcta aatttctatg tgtagcagca atgatacgaa cattgacttc
    16861 gatgctttta tttgaaccta ctctttcaaa acaacgttct tgtaaaactc ttaataactt
    16921 aacctgcata gctaatggca tatctcctat ttcatccagg aatagagttc ctccattggc
    16981 caactcaaaa cgtccttgcc ttgatgtaat cgctcctgta aatgcacctt tttcatgacc
    17041 aaataattca ctctccaata attcccccgg gattgctcca caattgatag ggacaaatgg
    17101 tttatttgct cgggatgaaa gagaatgtat gttgcgagca acgacttcct taccagtacc
    17161 tgactcaccc aaaatcaaca cactggcctc agtatcagct acttgctcaa ttaatttgcg
    17221 tacttgacga ataccttcac tattcccaac caagcttcta aataaaggag tcttatgggt
    17281 acctgcagtg ataaatttaa ctgcttcttt agctatttga cacctatgga gcgatttaag
    17341 catttttgca tagctgaatg gaaatgataa acaatcaacg atatttcgat ccgcgcattc
    17401 agatgtagaa agcacagcat caataaccaa tattggagtt tttggaaact ttttaactaa
    17461 tgtatccaat tcatccatca ttttttcaaa agaagtacac gctccaagaa ctatcacact
    17521 tggattagga ttaacactca attgccaatt gccatattga gtgacctcag agaattgtcc
    17581 tataaaatca aaaatagtcc gaagcttgtc acaacgttcc ttatcatcat cgataatata
    17641 aatcctgtca ttgctcctca taaacctatc cttaagtttg agtcatttaa tttaaagtat
    17701 cggacacata atgaataact gtcaaataat tgtcacaatt tattttaacc gtcataaaat
    17761 ctatcaaatt aggccgtatc gctataatca aaaaaatact aacctatcca gatgctaatt
    17821 aaaaaatcat agtcatcacc agactaagta tttgatttat ctgtaacaaa taatcatctt
    17881 aaacgcatta cactgattac caaaacataa ttcctgactt cctatggtct tcgtagcact
    17941 cacttgagcc tgcttcacga aatccttggc ttgcatatat cccatacgat tacagaacga
    18001 attggcagct tgtttaccac agcctttatt gcgctccata caccaatcca ctcggtaatc
    18061 attataacgg ggtacggcaa attttttctc tcgataatgg taaggtttgg gaggagtatg
    18121 cgataaacca atagcacaag ctattgtcat aaaaccatta caacgccatc ctttgcattg
    18181 agccttgcta cccaagtaat tagttaaacc gacgttatag gcaatgacat tttgagtgga
    18241 gtaatcataa cccagcattt ggcaataacg attcgctacc tcctttccgc actctttccc
    18301 atctaaagta cagtaatcaa gtcgttgccc taaataagta ggatgccaaa aatttctaaa
    18361 tgccaaattc tgcttacttg aagctgttaa ctgaccgcaa aaagaaaaaa ataaaagtat
    18421 aaaaaccagg aaatttctaa aattgctcat tataacaatc cattaccaag tattagcaca
    18481 tgttagctta ggatgactgg gaggcacaag agagatttat tcttctttta ctcgtactgt
    18541 aatgaacttt tgtcttattg cattttctac atacgactat gatttaaaag ctgaccaaag
    18601 acaaaacaac tatatcaaga taatttttca aagactataa acaatgttat aaatagcata
    18661 tgaacagaac ttatccctgg aatcaaggaa acaaatgttt ttaataagaa agtttggcca
    18721 aattgaatga cagaattaaa aacactttgt aacaaagagc ataggggctt aagcaaattc
    18781 taatgaaaca agcttttcaa tcatcacttt aaaagcccat atcaaaggct agtcgtttat
    18841 gaaaaaatat ttaactttta tagtgtcagg cttagcctta attgcaataa tatggttcat
    18901 tcgccactta tggataattg agactgtcaa ggttgtgcat cctgtttatg gaacagcagt
    18961 tgaagctgtc tatgccacag gaacagtcga accaaccgtc atgatgccaa ttgcctcaca
    19021 aaatacagcc cgccttatca agttgaatgc agatgagggt tcggaagtag taaagggaca
    19081 gatattagcc aacctggaag atgaacacct gcaacataac ttaaaagaac tgaaagagca
    19141 tgaaaaatta gcaaaaataa gatacgagcg gaatgctcaa ttagttaaaa aaggagtcat
    19201 ctccaaagat gagtatgatc gcaccttgtc cgaatggcaa gcagccagtg ccgcagtacg
    19261 cagaatagaa gctcaaatca gttatttaca actgatagcc cctgcagatg gccgtatcat
    19321 taaacgtgac ggtgaagttg gccaactaat cccggcaaat caacctgtat tttggctttc
    19381 ttgctgtgcc cccctaagag tttccgctga agtcgatgaa gaagatatcg tattggtcag
    19441 agttgggcaa aaagtattga tcagatcgga tgcttttcca aatcaaatct tttatggccg
    19501 tgtcaaaagc atcaccccta aaggcgatcc aatagccaga agttacagag ttcgcattag
    19561 tttttcagaa aataatcctt tattgattgg tatgacagct gaaaccaata ttattgtcca
    19621 tgaaaagaaa aatgccttat tgcttcctgt aagtagtatt tctgaaaaca aagtttggat
    19681 tgtacaggat gatgggaagc tctctcaaaa tacagtgact attggcgcaa gaggattaaa
    19741 acaagccgag attttaaaag gcgtcaatat taacgacctg gttgtcttac aacctgataa
    19801 taagctaaaa aatgggcaaa aagtgcgttt cattcttgta aaacaggaag aataatgaag
    19861 ctatttttaa agatcgcttt aaaacatttg cttgcacgca aacgacaaag cctggtttct
    19921 ttgatgggaa ttgtattggg ggtagccttc tttttaacaa tttcgtcact gatgcagggg
    19981 tctgaaaaag atttcatcaa acgcttggtg gataattcac cgcatatcac gatcatagat
    20041 gaatatcgaa accctcgtat acaacccatc caacagctgc acacacatgg cgctattgaa
    20101 attcgcagtg ttaaaccatt aacagaacct cgagggatta gagggtatga acaaacaata
    20161 aataatttat cgcttaccat cccgggaatt gttatttctc ctgttctaat tggacaagcc
    20221 ttaattaatt atgcagggaa agattattcg gtcactctga atggtatgat ccctgaagag
    20281 ataaaaaaag tttctactat cgaaaattat atgacttcag gttcgattga tgatctcata
    20341 gtcaatcccg atggtattgt cttgggaagc gagctcttgc gtaaattatc cctgacaaaa
    20401 gatgataaca ttactgtgac agcgcccaat ggtcaaatac gcactttcaa aattcttggt
    20461 gtatttcgca ctggccgttc agattttgat accaatcaag cgtttattag tattaaacgc
    20521 gctcaagcat tattaaaccg acctaaccgc tccaataaca tcattataaa actgcctaac
    20581 ccctatcagg cctatgacat tgccgctcaa attgaaaaac agataggcta taaaactatt
    20641 tcctggcagg aaaaatcgga agatttgctc aatacactag tcattcgaaa tatcattatg
    20701 tatagcgtgg ttagcgctgt tttaattgtc gctgcatttg gaatttataa tgttatttcg
    20761 actgtagtaa tggaaaaaca cagagacatt gccatactta aatcaatcgg tttccaaaaa
    20821 catgatattc aatttatttt tatcatagaa ggttttcttc taggactggt tggatgtttg
    20881 ttaggactgc ctttgggaag tggactgatg tacagtctca tgcaagtcca attcaagccg
    20941 ccaggaagct ctgaattgat taatatgcca ctcgattgga gttatttaca atttgtcatc
    21001 gccacagctt ttgcgatgtc cgcctcaatc atagccgccg cccttcctgc tcataaggca
    21061 gcgcttgttc atcctgttga tatcctgcga ggagggggaa tgtgaacaag attgtcataa
    21121 aaacaaaaga gctttcgcgt caactgtcag gtgaagttcc cgtcacttta gtcaaagaca
    21181 taaatctcga agtcagagaa ggtgaatttt tagtaatttc tggcccctca ggttctggca
    21241 aatcgtcatt gctttatttg ctaggcttgc ttgatagacc ctcccaagga acaatttggc
    21301 tgaataatga agatgtttca acttatgagg aagaaaaaat ggcctggttg cgcttgactc
    21361 aaattggtta tgtctttcaa ttccattttc tattacctga gtttacagcc ctggagaatg
    21421 tgatgttgcc tatgcaacgt ctaggcaaac taagtctggg ggaaatgaaa aaccgagcta
    21481 ccaagctttt aaccagccta gggttagagg agcaattatc aaaactgcct aaacagctgt
    21541 caggagggca aagccagcgc gtcgctattg cacgagctct ggccaatgat cccatattgc
    21601 tcttggcaga cgagccaact ggaaatcttg ataccgtttc aagtaccaat gtgcaaaata
    21661 tactaaaaga acttgctcat gaatacagaa gatcggttat catcgtaaca cacgatatga
    21721 attttgctgc tacagcagac agagtgattc acattgtcga tggcaaaatg caagaataac
    21781 cttacttctt gctagtccat gcttacttca tcagtttcat cctgatctat tcttcaggac
    21841 taacgaaaaa ccccaaggtc gaggcaaaaa atgtttttaa tgagggagtt tagataaact
    21901 aaatcaccga attaaaaaca ttttttaaca tacagattgg ggttttgcgt aggtcctgtt
    21961 ctttttaaat cggattagga ctatctataa accaatgttc caagtcgaaa tagtttgcaa
    22021 gataaacacc gagggattga atgccgaacc gttccgtcgc atgatgacca caagaaaaat
    22081 attgtatacc caattccata gcctgataga acgttcgttc agaaacttca ccgcttaaat
    22141 aagcatcaac gccaagttgg taagcttctt ctataaagtc ctgtgctgct ccagtgcacc
    22201 atgctatgga gtgaatcatt ttttcatttc ccgcaatatg cacaggatac cggccaagtt
    22261 tgtgctctaa aaaactggaa aactgttcgc tgttcattgc ctttgataat tttccagacc
    22321 aaaggagatt ggcagtattg ttgaccttat gcatctcatg ggactctatt aacagcaaat
    22381 tcgccaggct ggcattattc cctagctcgg gatgacaatc caaaggcaaa tgataggcaa
    22441 ataaattcat gttatggcca agtagcttag caatccttcg ccttttcatt ccggaaataa
    22501 caggattttc acctttccag aaataaccat ggtgaactaa cagtgcatca gcttgctgtt
    22561 caatagcttg ggaaatcacg tcctctgatg cactgactgc cgtacatata cgcttaatct
    22621 tgtctttgcc ttcaacttga attccattag gtgcataatc ctggtattga gagcaattca
    22681 agaaatccag taaaaaaagg cttaattcat ctcttgtaat cacctgtcag aaactctctt
    22741 aatgtccgct cctaatagag ataacttttc ttctattcgc tcatagcctc tatcaacatg
    22801 ataaatccgc tcgactactg tttccccttc agcaaccagg cccgcgagaa ttaaactggc
    22861 tgatgccctt aaatcagtag ccattaccgg tgcgccagtt aatttttcaa caccatgaac
    22921 gatggctgta tttccattaa gttggatatt agcccccatt ctctgtaact cttgaacatg
    22981 cataaatcgg ttttcaaaaa tagtttctat aatagttgaa gaaccttctg ctacggaatt
    23041 cattgccata aattgagctt gcatatccgt tgcaaatgca ggataagggg cggttgaaat
    23101 attaactgcc tgaggccgct tgttatgcat atttaaactg acccaatcct caccaatggt
    23161 taattcggcg cctgcctctt cgaatttaca taattgagat aataaagtat caggacgaac
    23221 tttcttaaca gtcacttgac cacgagtaag cgccccagct gccaaataag tgcccgcttc
    23281 tattctatcg gacatgaccg aataagtccc accgtttagt gattccacac cttctacttc
    23341 aatagtagaa gtacctgcgc ctcgaatttt cgctcccatt tgaatcagaa agttagccaa
    23401 gtctactact tctggttcgc gcgcagcatt tttaatcgta gtaatgcctt ctgccaacac
    23461 agcagccatt aacacatttt cggttcctgt gacagtaact gtgtcaaaca tcaagcgttt
    23521 accttgcaga cgtccctttt tgcaacgagc attgatgtag ccatttttaa ccgtaatgtc
    23581 tgcccccatg gctcttaatg cttttaaatg caggtcaact ggtcttgttc caatggcgca
    23641 tccaccgggc aaagaaacat ctgccttacc gaagcgcgcc agcattgggc ctaataccaa
    23701 aatggaggca cgcattgttt tgaccagatc gtaaggagca acaaactcat tgacttgact
    23761 ggagtcaact tgaacattca tcttttcatc aacaattaaa tgagctccca attgtcctaa
    23821 cagttccatc atggtagtga tgtctttaag atgaggtaca ttagatattg tgacgtgatc
    23881 actggctaat agactggcag ccatgattgg aagagcagcg ttttttgctc ccgaaatgac
    23941 cacttcacca tgtaatgctt taccaccatt aatcaataat ttatccattt tgcttccccc
    24001 attcttcttt tgtccatgtc ttcatactga tagcatgaag ccttccggtg gtaataaagt
    24061 ctttaagctg ggcataaacc cattgctgtc ttgctacttt agacttctta agaaatatat
    24121 cggtcactac agttacctga tattgataac catcgccctc taccttgaca taaaatacat
    24181 cgtcaatttc attaagtctt tgttcaattt cttcatta
//
LOCUS       DBNCWK010000032        23972 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.032, whole genome shotgun sequence.
ACCESSION   DBNCWK010000032 DBNCWK010000000
VERSION     DBNCWK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23972
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.032"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..377)
                     /locus_tag="AC06IO_001192"
     CDS             complement(<1..377)
                     /locus_tag="AC06IO_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874393.1"
                     /translation="MTRNSVKTVCIFLSIILYVIALCLPAAIIPDFERGIHQTHYGFE
                     ILITGWMGFLAWQPAWLANLLYLFSLLTYGSKNSYRFGITAFILALLSPFILVEKLLI
                     GFYVWLASFTVLLYGNNLHEITI"
     gene            complement(414..1457)
                     /locus_tag="AC06IO_001193"
     CDS             complement(414..1457)
                     /locus_tag="AC06IO_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIO9874394.1"
                     /translation="MITNNIRFNSIIFSLLFGLLSLLKINLLHADDTGEKNHLNLENY
                     SPQTKKLCQSVAYLSVPSQNQLDEASKASLKNCDALDLYYGFNAAPDYDKAFQCALMT
                     KDYNVLVMAYANGRGVKFNPDLAMHFACMIEDAAPAEMDGRIAHLAQIKEGNKKTSFD
                     ICDDITSGYMMGWCSSIDQRLEDVKRNKKINALVSQWTAQEQLLYQQVRKTAEVYIRD
                     HSMNEIDLSGTARSAFAINAQLGLNQSLFELLQKVDRCEAPLVTTKQYEELDRQLNSI
                     YKKLMADTTSFQYTTITKEGIKKTEIAWIKYRDAWIQLAHTKCPRISAESWQVILIQD
                     RIKLLNEILELAE"
     gene            complement(1484..1978)
                     /locus_tag="AC06IO_001194"
     CDS             complement(1484..1978)
                     /locus_tag="AC06IO_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2434 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874395.1"
                     /translation="MTIMMNKDKHSLLAIAAAEVPPRTKPSIYPEPFASMMAGRQKHA
                     LGDFFGIKNFGVNLTRLAPGAQSALLHKHKLQEEFVFILKGQPTLVTETADIQLHPGM
                     CAGFTPDGTAHQLVNRTADEVVYLEIGDRTQGDEVNYPADDLAAVFGNDGQWHFMHKN
                     GMPY"
     gene            complement(2244..4040)
                     /locus_tag="AC06IO_001195"
     CDS             complement(2244..4040)
                     /locus_tag="AC06IO_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874396.1"
                     /translation="MDIHMTPLFPEKNTPLTIKQGPVDDCYLLVALDCIFNSGDEARQ
                     LLASKFTQTPLGLTVRIKRNNQSTRLDSQKMRGKYIHVYDRNTDEDVFFIGQQKLKEI
                     DNSKKGVQTNSLAIKVLERISSYYYTGDWDHTGESGSLTAHSIRQRHMESSTRFVGKL
                     LGIHAEDTEEMDEIIKLKLMNPREAVYISFAYGEPDANGKIHERHALRIDKVLPKTNG
                     GYDFILVNPWDNQKQETFSLGELEKRDCRFCTFSLNQQRYEITRILLKNPVEQGQYIF
                     ANPELFTLLLQMQKLSLLSDAKNIQACINLHKQMPYLPSLYNSLTSDEQKKLIQGMVG
                     TKGDKEKFTKHLISNVPRLDLIKTIFQNEPLENKTENMIVDLALEAKKKHSPARIVFE
                     DPEFFTLIMNRAIHHKATIRGCSQEQAKKLIESKMIHYYFDNLRTDSLTRNARLCELF
                     AAKIFTKTSIEAWFAARLSLTNFDLSQALRTLGFSDQRSGVMSAPVLKKQTALVASQV
                     GFFPIYKSSPAPVANKSPAPTQELTAKKSSVPILPSIVKKPSTPLSEPIGKKLSTPIP
                     AFLGNGLSIYTPEKPSVKTLPPLKGVRLTSLP"
     gene            complement(4123..5034)
                     /locus_tag="AC06IO_001196"
     CDS             complement(4123..5034)
                     /locus_tag="AC06IO_001196"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIO9874397.1"
                     /translation="MKNLLFLVLVILTNSLMAKENLVFAVIGDYGANSAAEAKVASVL
                     KSKNPQFILTLGDNNYTHGCWKTIDKNVGKYYHEYIGNYQGNYGKGSDVNRFFPTLGN
                     HDWLARKTCLYQGTLPYFSYFTLPGNQSYYDFVRGPIHFFALDSDSHEPDGSKEGSKQ
                     YQWLTEQVQQSKAPFKIVYFHHAPLSSGKHGSNTRMQWNFASMGIDVVMSGHDHHYER
                     IERNGIVYFVNGAGGAELYSYKKWVEGSKFFYSKHHGFMLITALEHAIKIQFINENDE
                     IKDEIVIQEKNRVLENIRTYDKPQFPC"
     gene            complement(5155..6102)
                     /gene="mdcH"
                     /locus_tag="AC06IO_001197"
     CDS             complement(5155..6102)
                     /gene="mdcH"
                     /locus_tag="AC06IO_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
                     /protein_id="HIO9874398.1"
                     /translation="MKILFLFAGQGYHADSLFDIFRTDKKTINYLKIMSSVAEIDFLQ
                     HDLETTNPYSIQSIIGAYQLTLFSRLLPLFTNHQVDLAGYSLGEISAFLGSINADPRD
                     SFKLFRYRTQLMTSIVDNNIFAEYDLLSVVGKFILEEIQPVIAKHNCYIAIINSTQHV
                     VIGGKISDLRHLITALSQYPISHAKFLGVYLPSHTDFYSDKSKQFQQFLDENYGFYPL
                     KYPVISPLELNKIYDARQERVLLGQELCTTLQWHRVCSLIGEYQYNLIIDLGPGDSMT
                     TILNKANTELFNTPLITASHYNSLKGLCNAITSFISPYQ"
     gene            complement(6099..6941)
                     /gene="mdcB"
                     /locus_tag="AC06IO_001198"
     CDS             complement(6099..6941)
                     /gene="mdcB"
                     /locus_tag="AC06IO_001198"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HIO9874399.1"
                     /translation="MQIFQHSLPNPHKIARYFAKKAVQALYDEVALYPKPGLVSFIDS
                     GAHQDMNGALFFRSLFGLRHYFFQVGLHTALGYSPKKLVTFGLNAEKTMLKITGGINT
                     HRGAIFALGILCTSICKLSAKYHVFSAEDLHYAIIDQWAQYLEKEHQNSDTHGTLVTK
                     RYAVPDAKQTAIQGYKAVFHTYNELSNIIQDQTFFGLLAYQRLLLAIDDINILYRTGP
                     EGLEFARYHVQQDICQNNREKSIQKAIELHQLFSKKNISPGGVADMLGLIYFLRHVFS
                     REAK"
     gene            complement(6913..7536)
                     /gene="mdcG"
                     /locus_tag="AC06IO_001199"
     CDS             complement(6913..7536)
                     /gene="mdcG"
                     /locus_tag="AC06IO_001199"
                     /EC_number="2.7.7.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HIO9874400.1"
                     /translation="MNYLRHTLCYLDKNTTTASLPAPEQLDLLQYWLKCEYPLIVTRQ
                     PEDVLCGQIQLAIPYFIPTQSQKLRASYVVDDSWVIDTKALPSLHEIFPYLELDTSTK
                     IQVYGSYCWQHLTREQYIQSSSDLDVLISYENQSLLTLSELQKHLQKTLKIVCIDGEI
                     RFPALGDCSWAELLQIHSSDSILFKAAKKIFLVKRESLYANFPALLA"
     gene            complement(7533..8252)
                     /gene="mdcE"
                     /locus_tag="AC06IO_001200"
     CDS             complement(7533..8252)
                     /gene="mdcE"
                     /locus_tag="AC06IO_001200"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HIO9874401.1"
                     /translation="MMSNEKITLKQLIDYCFDTTKTTIKQQIVFGAGKIENKNFNIVG
                     TVEDTSFGVDESIEMARCILDIIKNNNTDPIVLLTDVAGQKLAVRDEWLGMYAYFAHL
                     LKCLHLARQNGNKIITLIYNQAIGGSFIAFGMMADRIYALKNAKLAVMWLEGMSKVTK
                     IDIDVLRKISETSPVFAPGVENFKKLGGLHQVLDLDEVSQQLLLTLQEPNISHDNRAE
                     LGKQYGGRKEAYDIIKAIENL"
     gene            complement(8253..9449)
                     /gene="mdcD"
                     /locus_tag="AC06IO_001201"
     CDS             complement(8253..9449)
                     /gene="mdcD"
                     /locus_tag="AC06IO_001201"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HIO9874402.1"
                     /translation="MDKYQYTFNLPQKLQISPMIKVGVAGSGNLEVIIKPNSNFDKTD
                     IIVNTVISGFRNTWDAVIERFVEDYPYGGLSITLNDAGATPPVVSLRLRQAMETYQTG
                     YPKKDSYTEANARTRIYSLVDEASFTEFLLDKETPSPTLPQLNMQVETDDGVIIGIAK
                     MDGIDIAIASQQKDFIGGSVGEIHGAKINGLIKYAIKHQLPAIIFLIDSGGVRLQEAN
                     VGEIEISEIIRSILDARSAGIKTIGVICGNNGAFGGMGIISGTLDYLIVNQGARIGIS
                     GAEVIQAVKGIEVFDSSNRPLVWRVYGGRTRFLKDDVQGYTTNKTMDIRQAIKTALKT
                     LSTTPSLSLNSILAEHEQLQKRITSAKNCHEESEWLKNNRPELYQQDIFSVSDQQFLE
                     LTNKGK"
     gene            complement(9458..11104)
                     /gene="mdcA"
                     /locus_tag="AC06IO_001202"
     CDS             complement(9458..11104)
                     /gene="mdcA"
                     /locus_tag="AC06IO_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HIO9874403.1"
                     /translation="MDWNKNLKDYESRLTNIKPFFKKGKIVSEKNIVALLEAAIKPHD
                     KVCLEGDNQKQADFLARSLCSVNPQKIYGLHMLQSAVTLSEHLDLFERGIAEKIDFAF
                     AGPQSTRLAIMIQRNQVKIGNIHTYNELYARYVMDLTPNVALITAEKADYYGNLYTGA
                     NTEETPSIIEATAFKNGLVIVQANEIVKKLPRIDIPGEWVDAIVQAPSPSYIEPLFTR
                     DPALIDEVKILMAMMVIKGIYAPYQVNRLNHGVGFNTCAIELILPTYAQSLGLKGKIC
                     KHWMVNPLPTLIPAIEAGFIESIFCVGGEVGMNEFIRSKPDIFFTGRDGSLRSNRLYG
                     QLAGHYGCDVFIGATLQMDPQANSSTATKGRIAGFGGAPNMGCDTLGRRHSSYAWLKA
                     GQEKNSHNPVAMPRGRKLVIQMVETFQGAGKPTFVEKLDAWELQKEMQADLPPIMIYG
                     DDISHVVTEEGIANLLLCKNSEEREQAIRGIAGYTPVGLKRDKAIVDELRHRGIIQRP
                     EDLSISLKEADRDLLAAKNIRDLVEISNGLYSPPNKFRNW"
     gene            11384..12190
                     /locus_tag="AC06IO_001203"
     CDS             11384..12190
                     /locus_tag="AC06IO_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874404.1"
                     /translation="MDMREKYTGRSEPIINKPSDIPKGLVAKRKAELLLAKLEEAIKA
                     QESLNKESESPVKESNSESLKPRLTHLTSERPKINHRPPSSFSYTNSDSTLFASVKEE
                     KTEREVSSVSFSNDKKKVEDNSSASIWTELMAALSRAVDNYTKKKMEGTTPGEAYGWF
                     SWLRHGKAGEDKAGGLASEARTTSTVESLLTLLHNFFADSATRYNNHSLAPYVMDELN
                     KLLVQYALKPFILSLGSQYDLNSWLHIKNQLSQLASKEEYQEKQSLVAPV"
     gene            12688..14031
                     /locus_tag="AC06IO_001204"
     CDS             12688..14031
                     /locus_tag="AC06IO_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874405.1"
                     /translation="MKEIIELLATLDELQKPYIHRVKTRLSELLNLDEKENELMRIEQ
                     STSRSWEEGATTDLRIGPIKSKKRIEEIRAVLIALSGANAVKYEEGHNLENNTVEFIV
                     TACDVEQLEGFIPLNQEEMQNTIPVMTFIRMSIAGNRGYVRGISFDSEKLDGENYLGS
                     FKEGELTASTQEWFSVLELLDLLNSESELNLIQIQNDLKSLLGVKFEVYFHSSSEEFP
                     IDLRTGSLDLKETGAQQLLRSMDSLVLLDCLKTENKELEDDQKVCIIKQVNPQELHRF
                     SLKKGKNTNANAEMLSNIIELLKRSFPNNQVIQDLVVFDGRDGRSMSRPYFHIRTQNY
                     HQEGTSDYKSAMSILKKLQDKTSKKIIFDYRNEMVEMKDESRPIYMSSIENTIKSSQF
                     IIDVAVGLTKQQLPELQNICAEINQSRLKHHRHNLFKPQIEEQKINEQCRVLFIG"
     gene            complement(14121..15815)
                     /locus_tag="AC06IO_001205"
     CDS             complement(14121..15815)
                     /locus_tag="AC06IO_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874406.1"
                     /translation="MKIILSILIALHATSTYAGQQVLVLSGGDNPGLNHYTQYLQTKT
                     LYEHLITRFNPETVSIYFGGGNTPTTQPSPFDVHKLINTKDNQQNKEDVMFTGIIANN
                     QQANKYNVDAFFLSSKIQQINPSDNLLLFVSDHGMPHGFLEDKNTNPYSNNCIDLWHY
                     QKPFINNFTNAKNFSKACLSKNELSSLLTLIPSQHVIFAMSQCYSGGFHQLSVTTQDG
                     YPVANTKICGFTSSTDDHYASGCTADANGATYQGYERSFTEWYTGHGVIDGKKIREPA
                     KSMLVAHQNAIIEDMTVDIPLSTSDYYLLQWAKLFASKQFKSRTKHYDNNMIQSIYLN
                     YKKRLQSSNNDALYQFMHVARASEDKIVQLMPEAREFASLSLTKQKQKISLLEKQLEQ
                     QGNELQDVWEGMMNLTLNVIMPSWEKAVNQSKSQPLNEKQQTFEKEFYQTIVKLNLYQ
                     HPYQFEMYYLQYLSEKQNDPDLVNYQNNRNAIIYQWAQAHNNDPLANAIKNWEKLNKK
                     QLSLTEHIAEEEKKKQWLKRIFTYNQIIASWVTLLTIHDEKALADLEGLLRCQNAS"
     gene            16099..18360
                     /gene="lem25"
                     /locus_tag="AC06IO_001206"
     CDS             16099..18360
                     /gene="lem25"
                     /locus_tag="AC06IO_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem25"
                     /protein_id="HIO9874407.1"
                     /translation="MSTSEKDVREQKVKTVTLSFLGTGQHREKVHHILTSFHNTISEV
                     NKDNPTVAIRMFDGPGSEPKSGDSKDPIPGTYIYNPKDNSKILISPVISQTITNAIQK
                     LTGNLAGEGIEHLLFEAVLYLNDIIEKNGGQLPETVNLHGFSRGADTCMRMANLLYQL
                     YPDIKVNLFLIDQVPGPGKRDDPHSYTVPPNVAHFESTLMLHEYRPGFDPQHSGRYVI
                     ADPEKTKVVVKPYYGEHNTGNRVTEDPNTNHTAILLHDDMNRFCRETGSLPSVGISPP
                     IIARVGDKKEEVRTHSELSPEKRFELLCGMKENEWGYAKLTKKYHERSILSKREDDVQ
                     DSRLFVNQEHRELFKQLYPKSFNWFFEKNHGGQTKKEEVITELKSLSEDPRYEHFFSS
                     LAKHFQINENNIAGTLPEPSGIDRIEKSIFRQPPVRDRLSYLQHSLTSIANYYHYHCD
                     EKNSTNESVKNLLLERVKESRSKPDSEAIKHLEQTMDEVRQTLESKNEKGFLWQQINH
                     ISPNARQYCEQVKAALREHLEHNQVLSDTQKEEIRKAMDRMDSIVNDSSKDSQQKYRE
                     IRREVIELNAKATTPEDDNQLTRSHFQKAYFELSGDKQKTLNLESLSQTLNQLSKAHY
                     GETNMTDKITQRLDGYKNRNWFWNSVKEVLNFFNIPLPKLHSEVKEQIADKLKERLVD
                     LKEKGMGNDVNAITRELGKAREDLIEHYKKTSKLEMGELDKIINKSMEELLVARKVTK
                     DLVHEEVSQVKLN"
     gene            complement(18459..19010)
                     /locus_tag="AC06IO_001207"
     CDS             complement(18459..19010)
                     /locus_tag="AC06IO_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg2420 family Dot/Icm T4SS effector
                     N-acetyltransferase"
                     /protein_id="HIO9874408.1"
                     /translation="MKKDITLFTSARPELKFIRLSECKEHLDTAAMWAKNAWGYMHLD
                     DEQFHYKQLQELCEHVYIAMYDGLPVGMLAVIEKTLSLEENREESSEKIVEDPSTPEI
                     IEMKNAYLDYVYIDERARSIGLAGIIIEKAKSLAREMDAEVMSLETLNSTLNGFYKRK
                     GGRVICESRYNDSYPTEVMRLKF"
     gene            19204..19998
                     /locus_tag="AC06IO_001208"
     CDS             19204..19998
                     /locus_tag="AC06IO_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIO9874409.1"
                     /translation="MNKKLSLIALCASSLSLHAGTMGEVVSPAYPWFASIGTGYSWTE
                     KPGIVNPNPVFWDFSLQGYDSDLGDRGFYTFAVGKQIHQYIDISLSYLDHEVFNYQKF
                     QTSLPGTSGTPGFTGSSRTRYFDLDNKALLVNGYLHPAQAWTRLASIDLTPFIDGGIG
                     YARNEVRNFYTVGTATVAGVAIGSTSSIGEPVNKNSFAWQASAGLNFHPTGSHLSIDA
                     GYRYYDGGQFNGPSVVYTNSDGFQVGTPWSGRLKANQIFVDFKYTV"
     gene            complement(20128..21819)
                     /locus_tag="AC06IO_001209"
     CDS             complement(20128..21819)
                     /locus_tag="AC06IO_001209"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIO9874410.1"
                     /translation="MFVLVACFCNQKILAAIPLVELIAKPNPTQFVKTQSSVLLFYTL
                     RNNTSVGFPLSYLFSSNNASLSNAGNTCGTFIGPNKTCQFTVQYQAPAISITEKLIVQ
                     VAYQGRAPVSDIVSINVDNNIACTLLNTASYQTPFCQQQYQKVLQFTPGVFNTTNQNV
                     QEEQTLGGIFGLYQNTGSTEQICYISCGLRSLNGTPPNENTLFELASVTKTFTGSILG
                     KFIINGTIASQFDEIRNYPHPAGLSFNVNEETVSFQQLATFSGGVCFSDAPSVNQSST
                     DQAVNQMNFITDINALNPDPNSGNCSNGAPNVRPVYATPPFLPTHNFYSNSSVGLLGQ
                     SLIRIDGFANVLEPDFNNWMCKNVTNPLDMIRTSACLPNEAHDGTCGTAAANCAYTAN
                     WLTAEYASGYHLFQNSYQLGNPFPFLPWAPAGGIRSNASDMIKFIRANLGFNTGVHNP
                     EQAQLIQGMLMAHEPNIYFPGGTEPNIGNQSPIRGGQGYAWVCMPSPPNGDRICGKIG
                     GHKNFRSFLGLNKSKNYGLIILFNTGALTTDGSLIPATKPPGISQIGVNLLENVN"
     gene            complement(21864..22592)
                     /locus_tag="AC06IO_001210"
     CDS             complement(21864..22592)
                     /locus_tag="AC06IO_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIO9874411.1"
                     /translation="MDKCIGFRIASIVLYALWSFSSYGTPIFRDHSLTLSGLIGYTDF
                     KDPIDQQLVISHYVTDLLTNSHKHDHPSVLISAKQQLNFEANKIHKIMLGPALYFQKL
                     HYSGDVWELMLPEFYNYQYDYTSNNVNFLIEGDMYFNPVSTWLIPFVTAGLGFGVAYV
                     HYDDYALPGIDPVSERHHFDSSIKGIYQLGAGLLVPVNPNWSINLRYAYIHMGKVHTT
                     PNSLGPIGLVLNNQNILVGINYSI"
     gene            complement(22824..23930)
                     /locus_tag="AC06IO_001211"
     CDS             complement(22824..23930)
                     /locus_tag="AC06IO_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874412.1"
                     /translation="MYNLTIHNLENYEKDPKIRLIPWALWENLFQHFISVYELSLMTL
                     SYKEAIHIFLPRTKNMEQLRQLLCLYYAHFDRNDKQFWCDVHKKGIKSEVICCAAAIT
                     GCSSALDTISLSLMPDEIVKMIQAENYYASRLAAENGHLHVLNRLCELAPTEVMAMIR
                     AENYHAFRLAAENGHLHVLNRLCELAPTEATAMIQSENYYAFRWAAVGRGHHNVINFL
                     LDCPAMLGYAEMHEFEYGEKYVNPFIARHVNRLKEMHDAFKQSNLEDLFDLVTKSECL
                     QGFYMLRNLIRRNDEALLDDIRFLLSIPGIKALAPAGTIPGNENELLRLALRLGNQGA
                     CALLLSIPSVLALTKANNYYIDETGGRLDLRAVA"
BASE COUNT         7261 a         4373 c         4776 g         7562 t
ORIGIN      
        1 tctatagtta tttcatgcaa attattcccg tatagtaaga cagtaaaact ggcaagccat
       61 acgtaaaatc cgattaataa tttttctaca agaatgaatg gcgaaagcaa ggcaagaata
      121 aaagctgtaa tgccaaagcg ataactgttt ttggatccat aggtcagtaa tgaaaataga
      181 tacaaaaggt ttgccagcca agcaggttgc catgctagaa aacccatcca ccctgtgatt
      241 aaaatttcaa atccataatg tgtttgatgt attcctcgtt caaaatcagg aattattgcc
      301 gcgggtaagc acaaggcaat aacataaagg attatcgata aaaagataca tactgttttc
      361 actgaatttc gggtcattga ttactcgctt tatttgatag tcaataacat taattattca
      421 gctagctcaa gaatttcatt gagcaattta atgcggtctt gaattaagat cacttgccag
      481 ctttctgcgc tgatcctggg gcatttcgtg tgagcaagtt gaatccaggc atctctatat
      541 ttaatccagg ctatctcggt ttttttaata ccttctttgg taatagtagt gtattgaaag
      601 gaggtagtat ctgccatcaa ctttttatag atactattca attgcctatc cagttcctca
      661 tattgctttg ttgtcacaag aggagcctca catcgatcta ccttttgtaa cagctcaaac
      721 aggctttgat tcagacctaa ttgcgcattg atggcaaatg cgcttctggc agttcctgat
      781 aaatcaattt cattcatcga atgatcacgg atatacactt cggcagtttt tctaacctgc
      841 tgatataata attgttcttg agcagtccat tgactgacta aggcattaat ttttttattg
      901 cgttttacat cttccaggcg ctgatcgata gaagagcacc aacccatcat atacccactg
      961 gtaatgtcat cgcaaatatc aaaagaagtt ttcttattac cctcttttat ctgtgcaaga
     1021 tgggcaatac gaccatccat ttccgccgga gcggcatcct cgatcatgca ggcaaaatgc
     1081 atggcaagat caggattaaa ttttactcct ctaccattgg cataggccat aaccaggaca
     1141 ttataatctt tagtcattaa tgcgcattga aaggctttgt cataatcggg ggctgcatta
     1201 aaaccataat aaagatcaag cgcatcacag ttttttagag aggccttgct ggcctcatca
     1261 agctggtttt ggcttggtac tgataaatag gccactgatt ggcataattt ctttgtttga
     1321 ggagaataat tctctaaatt aagatgattt ttctctccag tgtcatctgc atgaagtaag
     1381 ttgatcttga gtaaacttaa taaaccaaat aacaggctga agataatcga attgaatctg
     1441 atgttgttag ttatcataat tttttctttc taattatttg ttattaatat ggcattccat
     1501 ttttatgcat aaaatgccac tgcccatcat taccaaagac tgctgccaga tcatcggcag
     1561 gatagtttac ttcatcgccc tgggttcgat ctcctatttc aagataaacc acttcatcag
     1621 ctgtccgatt gaccaattga tgtgctgtgc catctggagt aaatccagca cacattccgg
     1681 gatgtaattg aatgtcagca gtttcagtga ctaatgttgg ctgccctttg aggataaaaa
     1741 caaactcttc ctgtaattta tgtttatgta gcaacgcaga ttgtgctcca ggagctaatc
     1801 ttgttaagtt gacgccaaaa ttctttattc cgaaaaaatc ccctaatgca tgtttttgac
     1861 gaccagccat catggatgca aaaggttccg gataaatgga aggtttagtg cgagggggaa
     1921 cctcagctgc tgctatagca agcaatgaat gtttgtcttt gttcatcatg atcgtcaatt
     1981 tgagttctat ccttgcaata tttaatcaat attagcataa agtaagttat cttgaatccc
     2041 tactttcagg aattctagat atggatttgt tactgaattt agcgaaacca aagcaagatt
     2101 aatcttaggc agcagaaagc aaagtataga gaaatatttt gagtgagtta gatttttcta
     2161 tgaatctgtt tccagatctt tgctttagga gttacaggtt aaaaaattaa tttccagcca
     2221 gcacaataga tatattatga attctagggg agtgatgtaa ggcggactcc ttttagagga
     2281 gggagcgttt ttacagaggg tttctctggg gtataaatgg ataatccatt ccctagaaat
     2341 gctggaattg gtgtagataa tttttttccg ataggttctg aaagcggagt agatggtttt
     2401 tttactatag atggaagaat aggaacagat gatttttttg cagtcagttc ctgggttgga
     2461 gcaggagatt tatttgctac tggtgccggt gaggatttat agattgggaa aaatccaacc
     2521 tggctagcca ccaaagctgt ttgctttttt agaaccggtg cgctcattac tccagaacgt
     2581 tgatcagaaa atcccagagt tcgtaatgct tgagacaaat caaaattagt caaagaaagt
     2641 ctggcagcaa accaggcttc aatggatgtt tttgtgaaaa tctttgcggc aaataattca
     2701 catagccttg catttcgagt caggctatct gttctcaagt tatcaaaata gtaatgaatc
     2761 attttggatt caatcaattt ttttgcttgt tcctgactgc atccccgaat agttgcttta
     2821 tgatgaatcg ctcgattcat gattaaagta aaaaattccg gatcttcgaa aacaatgcgt
     2881 gctggtgaat gtttcttttt tgcttcaagc gccaggtcaa caatcatgtt ctcagtttta
     2941 ttttccagag gttcgttttg aaaaatcgtt ttaatcaaat ccagtcgagg aacgttcgat
     3001 atcaggtgtt ttgtaaactt ttctttatca ccctttgtgc ctaccattcc ttgaatcagt
     3061 tttttttgtt cgtcagatgt taatgaattg tatagggagg gcaagtaagg catttgtttg
     3121 tgaagattaa tacacgcttg aatgttttta gcatcggata acagacttag cttttgcatt
     3181 tgcaagagca gggtaaacaa ttccgggttt gcaaaaatat attgcccttg ctctaccgga
     3241 ttttttaata aaatacgtgt gatttcatat ctctgttgat tgagactgaa ggtacaaaaa
     3301 cggcaatctc gtttttcaag ttctcctaaa ctgaatgttt cctgtttttg attatcccat
     3361 ggattgacta aaataaaatc ataaccacca tttgttttag gaagcacttt atctattctt
     3421 aatgcatgac gctcatgaat ttttccatta gcatctggct caccataggc gaagctgata
     3481 taaacggctt cccttggatt cattaatttg agcttgatga tttcatccat ttcttcagta
     3541 tcttcagcat gaatgcctaa taacttaccg acaaaacggg tggaggattc catatgtctt
     3601 tgtcttatgc tatgagccgt tagacttcca gattcaccgg tatgatccca atctccggtg
     3661 taataatagg aactgattcg ttctaaaact ttaattgcaa gtgaattagt ttgtacccct
     3721 ttttttgaat tatcaatttc tttcagcttt tgctggccta taaaaaaaac atcttcatct
     3781 gtgtttcgat cataaacgtg gatatatttg ccacgcattt tctgagaatc tagtcgggta
     3841 ctctgattat ttcgtttaat gcggacggtt agcccaagtg gagtttgcgt aaactttgat
     3901 gctaacaatt gacgagcttc atctccagag ttaaaaatac aatctaatgc gactagcagg
     3961 taacaatcat cgactgggcc ttgttttata gtaaggggag tattcttttc tggaaacaaa
     4021 ggcgtcatat ggatatccaa taatacaaaa agtatttaat cttgccattt tatcaaagaa
     4081 aggttatgtg aatattaagg aaaaccgagg caaaaaatgt ttttaacatg gaaattgggg
     4141 tttatcgtaa gtcctgatgt tctccagcac cctgtttttt tcttgaatga ctatctcatc
     4201 cttaatttca tcattttcat tgatgaattg gattttgatg gcatgctcca gagctgttat
     4261 cagcataaat ccgtggtgtt tagagtaaaa aaatttgctt ccttccaccc attttttata
     4321 ggaataaagc tctgctcctc cggctccatt gacaaaataa acaattccgt tgcgttcgat
     4381 acgctcgtaa tgatgatcat gaccactcat aaccacatca atacccatag aggcaaaatt
     4441 ccattgcata cgggtattgg aaccatgttt accagagctc aacggagcat gatgaaaata
     4501 gactatttta aaaggggctt ttgattgttg tacttgttca gttaaccatt gatattgttt
     4561 tgaaccttcc ttgcttccat ccggttcgtg agagtcactg tccaaggcaa aaaaatgaat
     4621 tgggccacga acaaaatcat aataactttg gtttccagga agggtaaaat aactgaaata
     4681 aggtaatgtt ccctgatata aacaggtctt tcgggcaagc caatcatgat tacccagagt
     4741 agggaaaaag cgattgacat cagaaccttt cccgtagttt ccctgataat tgcctatgta
     4801 ttcgtgataa tatttaccca cattcttatc aatggttttc cagcaaccgt gagtgtaatt
     4861 attatctcca agagtaagga taaactgggg attttttgac ttgagaacgc tggctacttt
     4921 ggcttccgct gcagaattgg caccatagtc tccaatgaca gcaaaaacca ggttttcctt
     4981 ggccatcagc gaattagtca ggataactaa aacaagaaac aacaaatttt tcaaagaaga
     5041 tccttgtgct tgatggagcg aagccgctat ggtaagagat ctctttttaa taaatcaata
     5101 gaaatgatta agttaattta tttttaaact ggtcgaaatt tagtaaatat tatttcattg
     5161 ataaggtgag ataaacgatg ttatggcgtt acaaagtcct tttaaactat tgtaatgtga
     5221 tgcagtaatt aggggtgtat tgaacaattc tgtgtttgct ttgttcaaga tagtagtcat
     5281 ggaatcgcca gggccgagat ctataatcaa attgtactga tattcgccga ttaaagagca
     5341 aactctatgc cattgaagtg ttgtacatag ctcttggcca agcaaaactc tttcttgcct
     5401 ggcatcgtat attttattta gttctagcgg gctgatgaca ggatatttta gaggataaaa
     5461 accataattt tcatccagaa attgctggaa ttgtttgctt ttatcagagt aaaaatccgt
     5521 atgagaaggc agataaactc ctaaaaattt cgcatgactt atagggtatt gggaaagcgc
     5581 tgtgatcaag tgccggagat ctgaaatttt tccgccgatc accacatgtt gtgttgaatt
     5641 gataatagca atatagcagt tatgttttgc aataacaggt tgaatttctt ccaatataaa
     5701 ttttccaacg acagacaaca aatcgtattc tgcaaagata ttgttatcaa ctatcgatgt
     5761 catgagttgg gtacgatacc tgaataattt aaaggaatct cgaggatctg cattaatgct
     5821 ccctaaaaaa gcgctgattt cgcctaaact gtaaccagcc agatcaactt gatggtttgt
     5881 gaagaggggc agcagtcttg aaaaaagggt taactgatag gcgccaataa tagattgaat
     5941 agaatatgga ttggtcgttt ccagatcatg ttgtaaaaaa tcaatttcag ctacagaaga
     6001 cattatcttt agatagttga ttgtcttttt atcagttcga aaaatatcaa ataaactgtc
     6061 agcatgataa ccttgcccgg caaaaagaaa taatattttc atttggcttc tcttgagaaa
     6121 acatgccgta aaaaataaat taagcctaac atatctgcta cgcccccggg acttatgttt
     6181 tttttggaaa ataattgatg cagttcaatg gctttttgta tggatttttc ccgattgttt
     6241 tgacatatgt cttgttggac atgataacgg gcaaattcta atccctcggg acctgtgcga
     6301 taaaggatat tgatgtcgtc aatagccaat aataaacgtt ggtatgctaa taatccaaag
     6361 aatgtttgat cttgaataat gttcgacaac tcattataag tatggaatac agctttatac
     6421 ccttgaatag cagtttgctt ggcatcaggt acagcatatc ttttcgttac taatgtacca
     6481 tgggtatcac tgttttgatg ctccttttcc aggtattgag cccattgatc aataatcgcg
     6541 taatgcagat cttcggcaga aaagacatga tattttgcac ttaatttgca aatcgaagta
     6601 cataatatac caagggcaaa tatagctcct cgatgagtat tgatccctcc agtaatcttt
     6661 agcatggttt tttcagcgtt caaaccaaat gttaccagtt tttttggtga atatcctaag
     6721 gcagtatgta ggccaacctg gaaaaaataa tgacgcaagc caaatagact gcgaaaaaat
     6781 aaagccccat tcatatcctg atgagcccca ctatcaatga aactcactaa tccaggtttt
     6841 ggatataggg ctacttcatc atacagggct tgaactgctt tttttgcaaa atatctggca
     6901 attttgtgtg gattaggcaa ggagtgctgg aaaatttgca tataggctct ctcttttcac
     6961 taaaaatatt ttttttgctg ctttaaataa aatggaatca ctggagtgga tttgtaacaa
     7021 ttctgcccaa gaacagtctc ctaaggctgg aaagcgtatt tcaccatcga tacaaacaat
     7081 tttaagcgtt ttttgtaaat gtttttgcaa ttctgacaag gtgaggagtg attgattttc
     7141 ataagatatc agtacatcca ggtctgaaga agattgaata tattgctccc tggtgaggtg
     7201 ttgccagcag taggacccat aaacctggat tttggtactg gtatcaagtt ctaaataagg
     7261 gaatatctca tgcagagaag ggagtgcttt tgtgtcaatt acccaagagt catccactac
     7321 atagctggcg cgtaattttt ggctttgcgt tggaataaag tagggaatgg caagttggat
     7381 ttgtccacag aggacatctt caggctgcct ggttactata aggggatatt cacattttaa
     7441 ccaatattgg agtaaatcaa gttgttcagg agcgggtaag cttgcagttg tcgtgttttt
     7501 atcaagatag catagagtat gtctgagata gttcatagat tttctatggc ttttataatg
     7561 tcataggctt ctttgcggcc gccgtattgt ttgcctaatt cggcacggtt atcatggcta
     7621 atattgggtt cctggagcgt caatagcaat tgctgagaga cttcatccaa atcaagaacc
     7681 tggtgtaacc ctcctaattt tttgaagttt tctacgcctg gagcaaatac cggggatgtc
     7741 tcactgattt ttcgtaacac atcgatatct atcttggtca ctttagacat accctctagc
     7801 cacatgactg ccaatttagc atttttcaaa gcatatattc tgtctgccat catgccaaat
     7861 gcgataaaac tgccgccaat ggcttgattg tatatgaggg taatgatttt attgccattt
     7921 tgacgtgcca aatgcaaaca ctttaataaa tgagcaaagt atgcatacat tcctagccat
     7981 tcatcacgta ctgctaattt ttgtccggct acatcagtca acaggacaat aggatctgtg
     8041 ttgttatttt tgattatatc cagaatacat cgggccattt ctatggattc gtcaactccg
     8101 aaactggtgt cttcaacggt gccaacaatg ttgaaatttt tgttttctat ttttccggct
     8161 ccaaaaacaa tttgctgttt aattgttgtt tttgtcgtgt caaagcaata atcgattaat
     8221 tgttttaaag taattttttc attggacatc atttatttcc ctttattagt caattccagg
     8281 aattgctggt cagaaacact gaatatgtct tgctgatata attcgggtcg gttatttttt
     8341 aaccactcac tttcctcatg gcaatttttg gctgaggtaa tgcgtttttg cagttgttca
     8401 tgctctgcca gaatagagtt cagactaagg gatggggtag tggagagcgt tttaagggca
     8461 gtttttatcg cctgacgaat atccatggtt ttattggtgg tatatccttg aacatcgtct
     8521 ttaagaaagc gggttctacc gccatagact cgccagacta atggacgatt actgctatca
     8581 aatacttcaa ttccttttac tgcctgaatt acttccgctc ctgatatgcc aatacgtgct
     8641 ccttgattaa cgataagata atcaagagtg ccactgatga tccccatgcc accaaaggcc
     8701 ccattattgc cacaaataac gcctatggtt ttaatgccgg ctgagcgagc atccaggatg
     8761 gagcgaatga tttcagaaat ttcaatctca cctacattcg cttcttgcaa acggactcca
     8821 ccactatcaa tcaaaaaaat gattgcaggc agttgatgtt taattgcata tttgattaaa
     8881 ccattgattt tggcaccatg tatctctcct actgacccgc caatgaaatc tttttgttgc
     8941 gatgcaatgg ctatatcaat tccatccatt ttggcgatac caatgatgac cccatcgtct
     9001 gtttccactt gcatgtttaa ctgaggcagg gttggacttg gtgtctcttt atcaagtaaa
     9061 aactctgtaa aactggcttc atcaactaat gaataaattc tggtgcgggc attcgcttcc
     9121 gtatagctat cctttttagg atatcctgtt tggtaggttt ccattgcttg acgcaggcgt
     9181 aaggaaacga caggcggagt ggcgcccgca tcgttaagag taatggatag cccaccataa
     9241 ggatagtctt caacaaagcg ttcgatcact gcatcccagg tatttcggaa accagagatt
     9301 acagtattta caataatatc ggttttatca aagttagagt ttggtttaat aatcacttcc
     9361 aaattacctg aaccggctac tccaacttta atcattggcg atatttggag tttttggggc
     9421 aaattgaatg tgtattgata tttatccata aataggtcta ccaattcctg aatttgttag
     9481 gaggactata aagtccattc gaaatttcca ctagatcacg gatgtttttg gcagcaagta
     9541 gatctcgatc agcttctttt aatgaaatgc tcaaatcttc tggtcgttga atgataccac
     9601 gatggcgcag ttcatcgact attgctttat cacgtttcaa acctaccgga gtataaccgg
     9661 cgattccgcg gatagcttgt tcacgctcct ctgaattttt acataataac agattggcta
     9721 tcccttcttc ggtgacgaca tgagagatgt catcaccata aatcataatg ggcggcaggt
     9781 cggcctgcat ttctttttgt agttcccatg catccagttt ttctacaaag gttggtttgc
     9841 ctgccccctg aaatgtttca accatttgta tcacaagttt acgtcctcga ggcattgcta
     9901 caggattatg agaatttttt tcttgtccgg ctttaagcca tgcgtaagag gaatgacgtc
     9961 gccctaaagt atcacagccc atattgggtg ctccaccaaa gccggcgatt cttcctttgg
    10021 ttgccgttga actgttggct tgcggatcca tttgaagagt cgcgccaata aatacatcac
    10081 aaccataatg cccggcaagt tgcccataca ggcgatttga acgtaatgag ccgtctcgac
    10141 cagtaaaaaa gatgtctggt ttggagcgaa taaattcatt catccctacc tcacccccta
    10201 cacaaaagat agactcgatg aatcccgctt caattgcagg gattaaagta ggtagaggat
    10261 tgaccatcca atgtttacaa attttacctt ttaaacccag agactgggca taagttggca
    10321 gaataagttc aattgcacaa gtgttaaaac caaccccatg atttaatcga ttgacttgat
    10381 aaggagcata aatgccttta atcaccatca tggccatgag aatttttact tcgtcaatga
    10441 gagcaggatc tcttgtaaac aaaggctcaa tgtaggaagg ggatggggct tgaacgatgg
    10501 catcgaccca ctctcctgga atgtcaattc ttgggagttt cttgactatt tcattagctt
    10561 gcacaatgac caggccattt ttaaaagcag ttgcttcaat aatactcggt gtttcttccg
    10621 tattggcacc ggtgtacaga ttaccgtaat aatctgcttt ttctgcagtg attaaggcga
    10681 cattgggagt aagatccatc acataacgtg cataaagctc attataagta tgtatattgc
    10741 ctatcttaac ctgattacgc tggatcataa ttgccaaacg agttgattgt gggccagcaa
    10801 atgcaaaatc aattttttca gcaattcctc gttcgaacaa gtcgagatgt tccgataagg
    10861 taacagccga ttgcaacata tgtaatccgt atattttttg gggatttaca gaacataggc
    10921 tgcgtgccag aaaatcggcc tgtttctgat tgtctccttc caggcagact ttgtcatgag
    10981 gttttattgc tgcttcaaga agggcgacta tatttttctc agaaacgatt ttgccttttt
    11041 taaaaaaagg tttgatgtta gtgagtctgc tctcataatc cttgaggttt ttattccagt
    11101 ccataatttt ttagtaatcg gatgagaact atagtgatat aaaatgttaa tagtatagta
    11161 gatattgacg caagtatttg attttatccc ctgctttttt cttgtggctt gtgcaaggta
    11221 agttggccta tgatatttct ctgtaactga aaaatacaaa atactttatt tcagcgtatt
    11281 tctgttttct ggttgaacag aagagattaa ttccttgaaa gaaaacaata tattgcatta
    11341 acattagtga actgtttaaa ttcataaatc atgacatagg gaagtggata tgagagaaaa
    11401 atataccggt cgatcagagc ctattatcaa taaaccttct gatattccta agggtttggt
    11461 tgcaaaaaga aaagcagaat tgctcttggc aaaattagaa gaagctataa aagctcaaga
    11521 atctttaaat aaagaatcag agagtcctgt gaaggagagc aattccgagt cactcaaacc
    11581 ccgattgacc catctaacga gcgaacgtcc aaaaatcaat catagacctc cttcttcctt
    11641 tagctatacc aattcagaca gtactttgtt tgcttctgtt aaagaagaaa aaaccgagag
    11701 ggaggtgtca tctgtatcct tttctaatga taaaaaaaaa gttgaagata actcttctgc
    11761 atcaatttgg acggaactga tggctgcctt atcaagagca gttgacaact atacgaagaa
    11821 aaaaatggaa ggaactactc ccggtgaagc ttatggttgg ttcagctggt tgcgtcatgg
    11881 caaggcagga gaagataaag caggagggct tgccagtgaa gcacggacaa ccagtacggt
    11941 tgaatctttg ttgaccctgc ttcataattt cttcgcagat tccgccacca gatacaacaa
    12001 tcattcattg gctccttatg tgatggatga gctcaataaa ttattggttc agtatgctct
    12061 taaacctttc atactcagtt taggttccca atatgatctt aatagctggt tgcatatcaa
    12121 aaatcaatta agtcaactgg caagtaagga agaatatcaa gaaaagcaat ctcttgttgc
    12181 gcctgtgtga ttatcaggag tcctgcacag gactccgtat tttttgatcg cttgagttta
    12241 acagtttaaa tttttttgaa ttgtttgatg attgcttgat tccaatcttt gcgatgcgga
    12301 aattgcagcg aatgcccaaa ttaaatgcgg ttgtgaatga actactaaaa cattgcctta
    12361 atagccttaa taaataaatt gtgaaggcat ccctgtcggc ggatataggt ataccaaaaa
    12421 aagtctcttt gagccggtct ctttcatttt ctgtacataa tttgattcat ttaaaacatc
    12481 accagttatt ttaacaattt tcgtgcttca ttatgggggc atggagtttt tattaagcag
    12541 agcacatgtt gtatttaaat tataaacatt gcgcttttta tatattgtat tttatgttat
    12601 tgttcaattt cttgatcaat aaaatcagga agttagatac aaaaactatt ttggagtagt
    12661 ccttttactt tacttggaat ttaaaagatg aaagaaatta tcgagttgtt agctaccttg
    12721 gatgaattgc aaaagcctta tattcatcgt gtcaaaacca ggttaagcga gcttttaaat
    12781 ctggatgaaa aagaaaacga gctaatgcga attgagcaat cgacaagccg gtcctgggaa
    12841 gaaggtgcta caaccgattt aaggataggt cctataaaat caaaaaaaag aatagaggaa
    12901 ataagagctg tgctcatcgc attgtctggc gccaacgcag tcaaatatga agaggggcac
    12961 aacttagaga ataatactgt ggaatttatt gttacggcat gtgatgttga gcaactggaa
    13021 gggtttatcc cactgaatca ggaagaaatg caaaatacta ttcctgttat gacgtttatt
    13081 cgtatgtcaa ttgcagggaa tagagggtat gtgcgaggga tttcattcga ttctgaaaaa
    13141 ctcgatggag agaattacct tggttcgttt aaagaaggcg aactaacagc gtcgacacaa
    13201 gaatggtttt ccgttcttga attattggat ttattaaata gtgagtcaga acttaatctt
    13261 attcaaatac agaacgactt aaaatctctt ttgggggtga agtttgaagt ttattttcat
    13321 tcatccagcg aagaattccc cattgatttg cgtacaggaa gcttggatct caaagagaca
    13381 ggggctcaac aactgctgag atcaatggat agcctcgttt tgcttgattg tttaaaaaca
    13441 gaaaacaaag aacttgagga tgaccagaaa gtatgcatta tcaagcaggt aaatcctcag
    13501 gaacttcaca ggttttcact aaaaaaagga aagaatacta atgcaaatgc tgagatgctc
    13561 tccaatatta tcgaactttt aaagcgatct tttcccaata atcaggtaat ccaggatctg
    13621 gttgtctttg atggcagaga cgggagaagt atgtcacgac cttattttca tattagaacc
    13681 caaaactatc accaggaagg taccagtgat tataaaagtg cgatgagcat tctcaaaaaa
    13741 cttcaggata aaacatcaaa aaaaatcatt tttgattaca gaaatgagat ggttgagatg
    13801 aaagatgaat caaggcctat atacatgagc agtattgaga atactatcaa gtcatctcaa
    13861 tttattatag atgttgcggt cggcttaaca aaacaacagc tacctgaatt gcaaaatata
    13921 tgtgctgaaa taaatcaatc caggttaaaa catcatcgtc ataatctctt caagccgcaa
    13981 attgaggaac aaaaaatcaa tgagcaatgt cgagttttat tcataggtta aagtcaaatt
    14041 cagcataaga ggcataaaat gcttcttatg tcgctacttt agccatcata ttttgttgtc
    14101 ttatcctgaa atattttggt tcaactggca ttttggcatc ttaacaaacc ttcgagatca
    14161 gctagagcct tttcgtcatg gatagttaat agtgtgaccc aggaggcgat gatctgattg
    14221 taggtaaaaa tccgctttaa ccactgtttt ttcttttctt cttcagcgat gtgctcggtt
    14281 aaggaaagct gctttttgtt tagtttttcc caatttttta ttgcgttcgc cagaggatca
    14341 ttattatggg cttgtgccca ttggtaaata atcgcgttgc gattattttg atagttcact
    14401 aaatcaggat cattctgttt ttctgataga tattgcaaat aatacatttc aaactgataa
    14461 gggtgttggt ataaattaag tttaacaata gtttggtaaa attctttttc aaatgtttgt
    14521 tgcttctcat tgagaggctg gctttttgat tgattcactg ctttttccca ggatggcatg
    14581 atgacgttga gtgtcagatt catcattccc tcccaaacat cttgtaattc gttgccttgt
    14641 tgctctaatt gcttttccag caaactgatt ttttgttttt gttttgttaa agataaagaa
    14701 gcaaattcac gggcttcggg cattaactgg actattttat cttcagaagc acgggcaaca
    14761 tgcataaatt ggtaaagggc atcattattg ctggactgaa gtcttttctt ataattcagg
    14821 taaatggatt gaatcatatt gttgtcgtaa tgtttcgtgc gggacttaaa ctgttttgat
    14881 gcaaataatt tggcccattg gagtaaataa tagtccgaag tacttaaagg aatgtctact
    14941 gtcatgtctt caatgatcgc gttttgatgg gcaaccaaca tacttttagc cggttccctg
    15001 attttttttc catcaatgac gccatgccct gtgtaccatt cggtaaaaga acgttcgtat
    15061 ccttgataag tagcgccgtt ggcatcagca gtgcagccac tggcatagtg atcatctgtt
    15121 gaggaagtga aaccacaaat tttagtgtta gctacaggat agccatcttg tgttgtaaca
    15181 gataattgat gaaacccgcc tgagtaacac tgagacatcg caaaaataac gtgttggctt
    15241 ggtattaagg ttagcagcga cgataattca tttttggata aacaggcttt gctaaagttt
    15301 ttggcgttag tgaagttgtt aataaatggt ttttgataat gccagagatc aatgcaattg
    15361 ttactgtagg gattagtatt tttatcctct aaaaacccat gaggcatgcc atgatcactg
    15421 acaaagagaa gcaaattatc actagggttg atttgttgaa ttttggaaga taaaaaaaag
    15481 gcatccacat tgtatttatt ggcttgttga ttgtttgcga taatacccgt aaacatgacg
    15541 tcttctttgt tttgttgatt atcttttgta ttaatgagtt tgtgcacatc aaatggagaa
    15601 ggttgtgtgg tcggtgtgtt acctcctcca aaatagatgc ttacggtttc gggattaaat
    15661 cgggtgatta aatgctcata aagagttttt gtttgcaggt attgggtgta gtggttaagg
    15721 cctgggttat ctcctcctga cagtaccaaa acttgctggc cagcataagt agaagtggcg
    15781 tgcagagcaa ttaaaatgct gagaattatt ttcatcttca ttccatgaat cgttgtttta
    15841 attcttttca ttgttgtgtc aaggatagca attcttgtat tgaactgcta atgataattg
    15901 aaacgttagg tttaaaggct aaggctaatt agatattggc aaaacatagt cagcacccag
    15961 cactgttatg gcttgtgttt tgattatcct tagagactta atcgccattt cagcttaatt
    16021 ttgattggtg catgtgcatt tgttgtgcgc atggattaaa attatatcaa ggggccccta
    16081 ttagttgata ttcatattat gagcacatcc gaaaaagacg ttcgtgaaca aaaagtaaaa
    16141 acagtcacat taagttttct gggtacagga caacatcgtg aaaaggttca tcatattctc
    16201 acgagttttc ataatacaat ctcggaagta aataaagaca atcctacagt ggcaattaga
    16261 atgtttgatg gtcctggttc agaaccaaaa tcaggcgaca gcaaggatcc aattcctggg
    16321 acttatattt ataacccaaa agataattcc aagatattaa ttagtcctgt aatctcccaa
    16381 acgattacca atgccataca aaaactgaca ggaaatttgg cgggagaagg tatagagcat
    16441 ttattatttg aggcagtact ttaccttaat gacatcattg aaaaaaatgg aggccaactg
    16501 cctgaaacag ttaatttgca tgggtttagc cgaggggctg atacttgtat gcgcatggcg
    16561 aatcttcttt atcaattata tcccgacatc aaggtcaatt tatttcttat tgatcaggtt
    16621 cccggacctg ggaaacggga tgatcctcat tcttatactg ttcctcctaa tgtagcacat
    16681 tttgagtcga cattgatgtt acatgaatac aggcctggat ttgaccctca acattctgga
    16741 aggtatgtga ttgcagatcc cgaaaaaaca aaggtagttg ttaaaccgta ttatggagaa
    16801 cacaataccg gtaaccgtgt taccgaagat cccaacacaa atcacaccgc tattttatta
    16861 catgatgata tgaatcgctt ttgcagggaa accggctcac tcccttctgt tggcatttct
    16921 ccacccatta ttgccagagt aggagataaa aaagaggagg ttagaactca ttcagaatta
    16981 tctccagaaa aacgctttga gttgttatgt ggtatgaagg aaaacgaatg gggctatgcc
    17041 aaactgacca aaaaatatca tgagcgcagt attctgtcga agcgggaaga tgacgtacaa
    17101 gactccagat tgtttgtcaa ccaggaacac agggaattgt ttaaacaatt gtatcccaaa
    17161 tcatttaatt ggttttttga aaaaaatcat ggcggtcaaa caaaaaagga agaagtcatc
    17221 actgaattaa aatcactatc cgaagatcca aggtatgagc attttttttc atcattagcg
    17281 aaacactttc aaattaatga gaataatatt gcaggaactt tgccagaacc atcgggcatt
    17341 gataggatcg aaaaaagtat cttcagacaa cctccagtca gggacaggct ttcttattta
    17401 caacattccc tgacctccat tgcgaattat tatcactatc attgtgatga gaagaattca
    17461 acgaatgagt cagttaaaaa tttgctgtta gaacgtgtca aagagtcaag gtcaaaaccg
    17521 gatagcgaag caatcaagca tttagaacag accatggacg aggtaagaca gacacttgag
    17581 tccaaaaatg aaaaaggttt cctctggcaa cagattaatc atatttcccc caatgctcga
    17641 caatattgtg aacaagtgaa agcagcactc cgggaacacc ttgagcataa tcaggtacta
    17701 tcagatactc aaaaagagga aattcgtaag gctatggata gaatggatag catagtcaat
    17761 gatagtagca aagacagcca gcagaaatat agggaaataa gaagagaggt gattgagtta
    17821 aatgccaaag ccaccacacc ggaagatgat aatcaattaa caagaagtca tttccaaaaa
    17881 gcttattttg aattatcagg ggataagcaa aaaactctga atttagaatc tctgtctcaa
    17941 acattgaatc aactatcgaa agctcattat ggtgaaacca atatgacgga caaaattact
    18001 cagcgattgg atgggtataa aaatcgaaat tggttttgga attcagtcaa ggaagttttg
    18061 aattttttca atattcctct tcccaaactg cattcggagg ttaaagagca aattgctgac
    18121 aagctcaaag aaagacttgt tgatctcaag gaaaagggca tgggtaatga tgtgaatgca
    18181 atcaccagag agcttggtaa agctcgcgaa gatttaattg aacattataa gaaaacttca
    18241 aaactggaaa tgggagaatt ggataagatt atcaataaat caatggaaga actccttgtg
    18301 gcacgcaaag tcaccaaaga cttggtccac gaggaagtta gtcaggttaa attgaactaa
    18361 actctgttct attctgagaa agctatcata agcaaggttt ttagtttgac aaataatatg
    18421 aatttggctg caagctgtag acaaaattgc tgctctgatt aaaactttaa tctcatcact
    18481 tcagtagggt aactatcgtt atatctgctt tcacaaatga ctcgtccgcc ttttcttttg
    18541 tagaatccat tgagggtgga gttcaatgtt tcaagagaca ttacttcagc atccatttct
    18601 ctcgcgaggc tctttgcttt ttcaataatg attccagcca gtccaatgct tcgggcacgt
    18661 tcatcaatgt acacatagtc caaataagca tttttcattt cgatgatttc tggagttgag
    18721 gggtcttcca caattttttc ggaggattcc tctctgtttt cttcaagaga gagagttttt
    18781 tcaatgacag cgagcattcc aaccggtagt ccgtcataca tcgcgatata aacatgttca
    18841 cacaattcct gcagttgttt gtaatgaaat tgttcgtcat ccagatgcat gtatccccag
    18901 gcattctttg cccacattgc tgcagtatcc agatgttcct tgcactcgga taatctgata
    18961 aattttagtt caggtcttgc tgatgtgaat aaagtgatgt cttttttcaa acttgtacct
    19021 cttgtgacta tgaattagca aatggaaaaa atgctatatg atactgttta tctgcacttt
    19081 atcaacccaa gatagtagtt tggcaagctt gctaattttt cttgttcatt taaactaaag
    19141 gccgttttca aggacgcttc ataatagtat gtctgttatg tctattcaaa taaggatatt
    19201 atagtgaata aaaaattgag tttaattgct ctgtgtgctt cttcgctttc tttgcatgct
    19261 ggaacaatgg gagaagttgt ttcacctgcc tatccatggt ttgcttctat aggcactggc
    19321 tattcctgga ctgaaaagcc agggattgtt aatcctaatc ctgttttttg ggatttttca
    19381 ttacaaggtt atgattctga tctgggggat aggggatttt atacctttgc tgttgggaag
    19441 caaattcatc agtatattga tattagctta agttatcttg accatgaggt ttttaactat
    19501 caaaaattcc aaacatcact acctggcact tctggtaccc ctggttttac agggagctca
    19561 agaactcgct attttgatct tgataataaa gcattattag taaatggcta cttacatcca
    19621 gcccaggcgt ggactaggtt ggcgagtatt gatttgaccc ctttcataga tggtggtatc
    19681 ggttatgcgc gtaatgaagt acgtaatttt tataccgtag ggacagcaac ggtagctggt
    19741 gttgctattg gttctacgtc aagtattggt gagcctgtta acaaaaacag ttttgcatgg
    19801 caagcgtctg ccgggctgaa ttttcatcca acaggaagcc atctgagtat cgatgcgggt
    19861 tatcgctatt atgatggcgg tcaatttaat ggaccatcag ttgtctatac caacagtgat
    19921 ggttttcagg taggaacacc ctggtcaggt cgtttgaagg caaaccagat ttttgttgac
    19981 tttaagtata cagtataatt attccgatca ggaaaattgg attactgcat tgtcgagcta
    20041 catggtttat gtagccattc acacatacca ggtataggag tgatgaacgg ttgcattcct
    20101 gtttcctatc tatcgaagca gaatgtatta attaacattc tctaataaat taacgccaat
    20161 ctgacttata cccggtggtt ttgtcgcagg aatcaaactt ccatctgtcg taagagcgcc
    20221 cgtattaaat aaaataatca aaccatagtt tttactttta tttaagccaa gaaagctgcg
    20281 aaaattttta tgtccgccaa tttttccaca gattctgtct ccattagggg gagaaggcat
    20341 gcatacccag gcatagcctt ggccaccacg tatagggctt tggttgccta tattaggctc
    20401 tgtgccacct gggaagtaaa tatttggttc atgagccatc aacatgccct gaattagctg
    20461 ggcttgttcg gggttatgaa ccccagtgtt aaagcccaaa ttagcgcgta tgaatttaat
    20521 catatcagag gcgttacttc gaattccgcc agcaggtgcc cagggtaaaa atgggaaagg
    20581 attgcccaac tgatagctgt tttgaaatag atgatatcct gaggcgtatt cagctgttaa
    20641 ccaatttgca gtgtaagcac agtttgcagc tgctgtacca caagtgccat cgtgagcctc
    20701 attaggaaga caagcgcttg ttcttatcat atccagagga ttggtgacgt tcttacacat
    20761 ccaattattg aaatcgggtt caaggacatt agcaaaacca tcgatacgga taagactttg
    20821 tcctagtaag cccacgctac tgtttgagta gaaattatgt gttggtaaaa atggcggagt
    20881 ggcgtataca ggtcttacat tgggtgcacc attactacaa ttgccgctat tcggatcagg
    20941 atttagcgca ttgatgtctg ttatgaaatt catttggttg acagcttgat ctgtactgct
    21001 ttgattgaca ctgggagcat cagagaaaca aactcctccg gagaaagtag ccaattgctg
    21061 gaaactcaca gtttcttcat tgacattgaa gctaagacct gcaggatgag gataattccg
    21121 aatttcatca aattgggaag caatagtgcc attgataata aatttcccta atattgaccc
    21181 ggtaaaagtt ttggtgacgg aggctaattc aaatagtgtg ttttcattgg gcggtgtacc
    21241 atttaaagac cttaatccac aacttatgta acagatctgt tcagtgcttc ctgtattctg
    21301 atacaggcca aatatccctc ctaaagtttg ttcttcttga acattttgat ttgttgtatt
    21361 gaatacgccg ggagtaaatt gaagtacttt ttggtattgc tgttggcaga aaggagtttg
    21421 ataggatgcc gtattgagta aggtacaggc gatgttatta tctacattga tagagacaat
    21481 atcgcttact ggcgcccgtc cctgataagc tacttggact attaattttt ctgtgatact
    21541 aatagctgga gcttgatatt gtacagtaaa ttggcatgtt ttgtttggac caataaatgt
    21601 tccacaggta tttccagcat ttgataggct ggcgttattg ctggaaaata aatagctaag
    21661 cggaaagcca acacttgtat tattgcgcaa agtataaaat aacaagacag agctttgggt
    21721 ttttacaaat tgagtggggt tgggctttgc aatcaactca accaggggga tggctgccag
    21781 tattttctga ttacaaaagc atgcaactaa tacaaacatt aataaataac ctgtcttttt
    21841 catatttggc ctgagttatt gagttatatc gagtaattaa ttcctaccag tatgttttga
    21901 ttgtttaaaa caagccctat aggcccaagg gagttagggg tagtatggac tttgcccata
    21961 tgaatataag catagcgtaa attaattgac caattaggat tgactggtac tagaaggcct
    22021 gctcccaatt gataaatccc tttgatagaa gagtcaaaat gatgtcgctc acttacagga
    22081 tcaatgccag gaagggcata atcgtcatag tgaacatagg caactccaaa tcccaaacca
    22141 gcagtgacaa aaggaataag ccaagtagat acaggattaa aatacatgtc tccttctatt
    22201 aaaaaattga cattgttact tgtataatca tattggtaat tatagaactc aggtaacatt
    22261 aattcccaca catcgccaga ataatgcagt ttttgaaaat acaatgcagg acccagcatg
    22321 attttatgaa tcttattggc ttcaaaattc aattgttgct tggcagaaat taatacgctt
    22381 gggtgatcat gtttatggga attagtcagt aaatcagtaa catagtgact gattaccaac
    22441 tgttgatcga taggatcttt gaaatccgta taaccgataa ggccagatag agtcaggcta
    22501 tgatcacgaa atatgggtgt gccataagag gaaaaagacc atagtgcata aagaactatc
    22561 gaggctatcc taaatccaat gcatttgtcc acagacgtcc tttttgagta ttcacaatga
    22621 ttaaaattca tacttacaat gaagcgaata aattatcatg aaataaaaaa ataggaagta
    22681 aaatgaggag aaaaaaatgt ttcttgtatt atatatcgag catggaaatt aatgctcaat
    22741 taaatttaga cgataatttt ctgttcgatc aggggtttca taaagtatca catcagatca
    22801 attgaagtga cttgaacgga atcttatgct acggctctta agtcgagtcg ccctcctgtc
    22861 tcatcaatat aataattgtt tgccttagtt aatgccagta cagatggaat agataatagt
    22921 aatgcgcaag cgccttgatt acccaatcgc agagccaggc gcagtaactc attctcattt
    22981 cctggaatgg ttccagcagg agccaaagct tttataccgg ggatgctcag caaaaaacga
    23041 atatcatcaa gtaaagcctc atcattacga cggattaaat tacgtaacat ataaaagcct
    23101 tgcaggcact ccgactttgt gactaaatca aagagatctt ccaggttact ttgtttaaag
    23161 gcatcatgca tttcttttaa cctgttcaca tgcctcgcaa taaacggatt gacgtacttc
    23221 tctccatatt caaattcatg catttctgca tacccgagca tagctgggca atcgagcaaa
    23281 aaattaatga cattatgatg gcctcttcca actgctgccc agcgaaaagc ataataattt
    23341 tctgactgaa tcattgctgt ggcttcagtt ggcgccaatt cacataaacg attcagcaca
    23401 tgcaggtgtc cattttctgc agccagacga aaggcatggt aattttctgc ccgaatcatc
    23461 gccatgactt cagttggcgc caactcacat aaacgattta gcacatgcag atggccattt
    23521 tctgcagcca gacgagaagc atagtaattt tctgcctgaa tcattttcac aatctcatca
    23581 ggcattaaag aaagagatat tgtatctaag gcacttgagc aaccggttat tgccgcagca
    23641 caacaaataa cctcgctttt tattcctttc ttgtgaacat cacaccagaa ttgtttgtca
    23701 ttcctgtcaa aatgggcata gtatagacac aacaattgcc tcagctgttc catatttttt
    23761 gtccttggta aaaagatatg gattgcttct ttataagata aagtcataag agataattca
    23821 tacacactga taaaatgctg aaataaattt tcccatagag cccatggtat taatcgtatc
    23881 ttcggatctt tctcatagtt ttctagatta tggattgtta gattatacat agagctaaat
    23941 aaataaaaaa caaactaaat aattataaat ta
//
LOCUS       DBNCWK010000033        23001 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.033, whole genome shotgun sequence.
ACCESSION   DBNCWK010000033 DBNCWK010000000
VERSION     DBNCWK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23001
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.033"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1195
                     /locus_tag="AC06IO_001212"
     CDS             <1..1195
                     /locus_tag="AC06IO_001212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874413.1"
                     /translation="TYSFILINFRRSSVMIEVNIWLSTAFLFKKRIKHKFFGPLLASE
                     DKGENVGHVNFTIEIDERTKNSFDFIEKHGPELGAKKTLRAVPTQATEPSMTNIESSH
                     LKPTMVKSDVVSHSFWPEGRPSKSETIKGKGVKPKFKTHEDDMIAEDSVTAMDITHRK
                     SALEDIDREKKKNLDLLVEISDLEVNMEQRSLFTDDLKKLHLEKEDLSKQQKQLTNSH
                     QTQLKDLKGQLAKLELHLNKTSGQIKATERTLAYLNKLENPDPKTRAQSIELTEKLIK
                     LKKEFETTISSQHEISELMSKSELTYHEGMQKIEKNLQQVELAINYREENLRQLDIKI
                     NGRDENDLKELQEEARHRKEYISRKEQFVKSRDFTKGRHILITVSHCQLRKVVLLIML
                     MKSKY"
     gene            1201..1449
                     /locus_tag="AC06IO_001213"
     CDS             1201..1449
                     /locus_tag="AC06IO_001213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874414.1"
                     /translation="MQEERSQNYSFLFNNCASSAKRCLLAGIDEKLRAKLKEAGLDSK
                     FFEISKVETCKSLRDWARTLESKLTELNFPPPAPTRGA"
     gene            complement(1514..1906)
                     /gene="erpA"
                     /locus_tag="AC06IO_001214"
     CDS             complement(1514..1906)
                     /gene="erpA"
                     /locus_tag="AC06IO_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIO9874415.1"
                     /translation="MASVDVSQNTSDIHFSVSAADKVAELIKEEDNSDLNLRVSITGG
                     GCSGFQYGFSFDEQINDDDTVIIQQCSDGKSSVKLLIDSMSYQYLHDAEIDYIKGIQG
                     EQFVIRNPNAKTTCGCGSSFSIGDEDDL"
     gene            complement(2041..3126)
                     /gene="mnmA"
                     /locus_tag="AC06IO_001215"
     CDS             complement(2041..3126)
                     /gene="mnmA"
                     /locus_tag="AC06IO_001215"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIO9874416.1"
                     /translation="MKAKVIVGMSGGVDSSVAAWLLKEQGYQVEGLFMKNWEQDDHND
                     YCPAAKDLADAQAVCNQLRIPLHTVNFSKEYWDRVFAYFLSEYEKGRTPNPDVLCNKE
                     IKFNAFLNHALTLGADYIATGHYAKNTIEGSIGYLFKAKDREKDQTYFLHAVEPEALS
                     KTIFPIGDFTKPQIREFAKQLGLVTHAKKDSTGICFIGEKRFKTFLNEFILAKPGEIK
                     STGGKTLGQHDGLMFYTLGQRQGLGIGGLQNSTDEPWYVVDKDIASNTLYVAQGSQHP
                     MLYSQGLICGPIHWLADYENHLPLTCFAKTRYRQTDQACMISPPDNNQHYVMFSSPQR
                     AITPGQFIVFYEKNQCLGGATIEQIIR"
     gene            3399..3827
                     /locus_tag="AC06IO_001216"
     CDS             3399..3827
                     /locus_tag="AC06IO_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIO9874417.1"
                     /translation="MMHLQELAKHGQQEKEVILNERLPSFVSPGCRLQASYHIEAKDD
                     FYLIHLRVQGNLNSICQRCMENFTVCYNNSTVIAVCRNDERAEQLLSQYECLVSSNWQ
                     VDLNELIIDELHLYAPQVHPDINECAKEINEILTGKKQTS"
     gene            3913..4104
                     /gene="rpmF"
                     /locus_tag="AC06IO_001217"
     CDS             3913..4104
                     /gene="rpmF"
                     /locus_tag="AC06IO_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIO9874418.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTKPTLSVDPTTGETHLRHHMTP
                     DGYYRGKKIIDAETAYEQE"
     gene            4110..5138
                     /gene="plsX"
                     /locus_tag="AC06IO_001218"
     CDS             4110..5138
                     /gene="plsX"
                     /locus_tag="AC06IO_001218"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIO9874419.1"
                     /translation="MKNITIAIDAMGGDHGLEIVIPACIRAIKNNPDLKLLLVGVQDK
                     ISASLKKHGMLSCQQFTIVHASEVVTMDELPSHALRNKKDSSMRIAINLVKEGRAQAC
                     VSAGNTGALMATARYVLKTLPGIDRPAIVSELPTMGGKTRVIDLGANVDSCAEHLFQF
                     AVMGSALIQAIENKPKPKIGLLNIGVEEIKGNDQVKRTAHMLAECSVMNYVGYVEGDH
                     FYSGDVDLVVCDGFVGNVALKASEGLAKLLLTVLKESFSRNWLTKIVGLIALPALKHL
                     KNRLDPSRYNGASLLGLNGIVVKSHGGANEVGFQHAIEQAVLEVKNNVVDLVRDQIND
                     FINQGLLL"
     gene            5135..6088
                     /locus_tag="AC06IO_001219"
     CDS             5135..6088
                     /locus_tag="AC06IO_001219"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIO9874420.1"
                     /translation="MKNAVISGTGSYSPERQMTNAELETMLDTSDEWIVTRTGISSRS
                     VAQEHETTSYMASRAAEQALEASGLDAEEIDLILVATCTPDYFFPSVACHVQHALGIK
                     RPIPAFDIGAACSGFVYAMDVAKQYIATGAAKHVLVVGSESMSRAVDWTDRSICVLFG
                     DGAGAVVLSASDRQGIMGSVLHSAYDSDKLLVLRNSTFEQDRATIGMRGNEVFKIAVN
                     IMGNIVDEVLEASHLKKSDIDWLIPHQANIRIIQAIAKKLSLPMSHVIVTIGNQGNTS
                     AASIPLALDYSIKNNQIKRDEILLIESFGGGMTWGAMVIRY"
     gene            6115..7062
                     /gene="fabD"
                     /locus_tag="AC06IO_001220"
     CDS             6115..7062
                     /gene="fabD"
                     /locus_tag="AC06IO_001220"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIO9874421.1"
                     /translation="MVKSAFVFPGQGSQSIGMLSDFMPQYTVVTDAFAEASDALGYDL
                     WDLVQNGPETKLNQTEYTQAAMLTADVAIYRLLMQLGVPQPQVMAGHSLGEYAALVCA
                     NSLSLKDAAQLVARRGQVMQNAIPLGEGAMAAIVGLSDEQVRSLCEQASTANHLVTPA
                     NYNALGQIVVAGHRVAVDKLIQLAEEAGARLAMIIPVSVPCHCPLLKNAAELFAEDLA
                     QIEFQVPSVDVISNVDLSIYHSTQHIRDKLKEQLYSPVRWVETVQLIQQRGIDLVVEC
                     GPGKVLNGLIKRIERNLTTISVYDTISLDQVIERLNIPV"
     gene            7074..7820
                     /gene="fabG"
                     /locus_tag="AC06IO_001221"
     CDS             7074..7820
                     /gene="fabG"
                     /locus_tag="AC06IO_001221"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIO9874422.1"
                     /translation="MINLEGKIALVTGASRGIGRAVAEKLARNGAFVFGTATTEQGAA
                     SINSFFEKEKLAGKGIVLDVTSTEQVENLMSTLSDNDQCPSILVNNAGITADNLLLRM
                     DDEEWYKVIETNLNSIYRMTKACLKPMFRARWGRIISIGSVVGASGNSGQVNYTAAKA
                     GVIGFSKSLAQEIGSRGITVNVVAPGFIDTDMTTALPDMVKEEMLKRIPMRKLGQPED
                     VADAVAFLASDSAKYITGETIHVNGGMYMS"
     gene            7905..8153
                     /gene="acpP"
                     /locus_tag="AC06IO_001222"
     CDS             7905..8153
                     /gene="acpP"
                     /locus_tag="AC06IO_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIO9874423.1"
                     /translation="MSTVEERVRKIVVEQLGVKEEELKNDASFVDDLGADSLDTVELV
                     MALEEEFETEIPDEKAEKITTIQEAIDYIESNLNKEEA"
     gene            8173..9411
                     /gene="fabF"
                     /locus_tag="AC06IO_001223"
     CDS             8173..9411
                     /gene="fabF"
                     /locus_tag="AC06IO_001223"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIO9874424.1"
                     /translation="MNSRRVVITGMGMLTPVGLNVEETWRSILAGVSGVGLVEDFDTT
                     EYPTKIWAKVKNFNVENHMPIKDARKMDVFTQYGMAAADEAMLDSGLKIDEVLSRRIG
                     VAVGAGIGGIQTITNNQDKLVAGGPRKVSPFFIPAGIINMVAGQISIKHNLKGPNISV
                     VTACTTGTHNIGLAGRIIAYGDADVMICGGAEMTTTPLCLAGFSAVRSLSKRNDEPEK
                     ASRPWDKDRDGFVMGEGAGILVLEEYEHAKARGAKIYAELVGFGMSGDAYHITAPDED
                     ADGASRAMEAAIQDAGIDPRQVDYINAHGTSTYLNDLNETKAIKRVFQNHAYDLAVSS
                     TKSMTGHLLGAAGAVEAIISILAIRDQVAPPTINLDNPDEGCDLNYVAHVPQSRTINY
                     VLSNSLGFGGTNGSLLFKRI"
     gene            9412..10410
                     /gene="mltG"
                     /locus_tag="AC06IO_001224"
     CDS             9412..10410
                     /gene="mltG"
                     /locus_tag="AC06IO_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIO9874425.1"
                     /translation="MQFSRHKKLVILVLSLFFMSFLFLFWNIYRLLYTPIIPKTASPM
                     IIALDKATSAYQFAYILQDKKLIHSAKFFLLIIRFEGLSHQLKAGVYQITPGETAMKL
                     LHRVVAGDVITQNFTIIEGSTQQKVDYDLRQANYLKYNPEDWAIVKENYQSAEGLLLA
                     DTYQYQGGSSSRALLEQAHRNLLNYLNTSWTNRAPNLPYKTAYELLIAASIIEKETAI
                     AQEKKLISGVMVNRLKKKMPLQMDPTVIYGLGNQYKGKLTHNDLLIQSPYNSYLNRGL
                     PPTPIAMVGKEAIDAAAHPQLSNYLYFVAKGDGTHQFSETYEQQRQAINQYRRKDY"
     gene            10410..11048
                     /gene="tmk"
                     /locus_tag="AC06IO_001225"
     CDS             10410..11048
                     /gene="tmk"
                     /locus_tag="AC06IO_001225"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIO9874426.1"
                     /translation="MSSLTGKLIVIEGLEGAGKSTAVNLVVELLSQKKISTITTREPG
                     GTKIGEILRSIIKNPEYNNVLDDRSELLLLYAARIQLIEQVIKPALNVGQWVIADRFE
                     LSTLAYQGGGRKMDMRVINELSNFCLKGFKPDLTLYLDINPELGMIRAKSRGKFDRIE
                     QESIEFFHRIHDTYHILVKQNPEIMMIDANRSLEEVQSSIQSVIEEFIEHNL"
     gene            11045..11950
                     /locus_tag="AC06IO_001226"
     CDS             11045..11950
                     /locus_tag="AC06IO_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HIO9874427.1"
                     /translation="MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKL
                     LLCKIKQNEPCLTCMDCQMIERFEHPDVEWVKPEKSGGIIKIDQIRGLQSTAFLTPQR
                     TNHKLIIIESAESMNIAASNALLKILEEPAQHTVFILLAQQIGNIPPTVLSRCQMTQF
                     AVFDELPCNNLLALGEYYTQESNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQH
                     ELSAFLWFLYLLYSQLQYMHLFRVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLR
                     KLSHNMNMNHTLVLEDLLFSLVVDY"
     gene            12077..12418
                     /locus_tag="AC06IO_001227"
     CDS             12077..12418
                     /locus_tag="AC06IO_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIO9874428.1"
                     /translation="MTEKPQLINCSFPNEASLYMAYMPFVKGGGLFIRTQHVYSLGQS
                     LELSVKLLNENEPYLVTGKIVWITPKGAQGNKPPGVGIQFTGENSRYLCNKIETYLAG
                     MLKSCQVTDTI"
     gene            12474..13262
                     /locus_tag="AC06IO_001228"
     CDS             12474..13262
                     /locus_tag="AC06IO_001228"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIO9874429.1"
                     /translation="MLVDSHCHLNFIDLTHFNHDLAQVINQARENGVEHFLSVCVELS
                     DYSQLERLAKDYPDISISVGVHPNSEMNYPVTAQMLCELAQNPACIAIGETGLDYYRN
                     PTEEAQSLQRDRFREHIKAALITSKPLIIHTRQAAEDTLLMMAKEGASQIGGVMHCFA
                     EDLDIAQRAIDLNFYISFSGIVTFKNAVSLQDVARQIPLERILIETDSPYLAPVPHRG
                     KQNHPALVKHVAEAIAQLRGMDFQEIAEITTNNFYNCFNIKRQG"
     gene            13374..14474
                     /locus_tag="AC06IO_001229"
     CDS             13374..14474
                     /locus_tag="AC06IO_001229"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIO9874430.1"
                     /translation="MSLYIGLMSGTSMDGIDAALLELPSNQLIHGITKQYSDDVRRNL
                     DDLIMGNHLTLASICQLNTLIGREFAEAVRQLLIEIKVHPKEIQAIGSHGQTVCHDTS
                     GSIPYTLQLGCGHTISSLTGITVVADFRTRDLVNGGQGAPFAPLYHQQIFSKVNESVA
                     VVNIGGIANVTFIAKNQMTRGWDIGPGNCLMDAWIYKNKGALFDKSGIWASQGEVIHP
                     LLEYLLQDPFFHLDSPKSIGKEYFSLSWLQKHLKPDYTPADIQATLLALTAHTIAETI
                     LNESGEIKQLYLCGGGAHNTHLKENLARLLPGIAVKSIAELGISPDYLEAMMFAWLAA
                     QTINQIPVNLTSITGAKGIAILGAVYPIIKSY"
     gene            14530..15822
                     /locus_tag="AC06IO_001230"
     CDS             14530..15822
                     /locus_tag="AC06IO_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874431.1"
                     /translation="MTAHYNVASNSGARNTLATSYFIFFLAASFYLYEFILQVAPSVM
                     AESMMKTFGVTGQGFGVISAFYFYAYAPTQLPAGVLYDRYGPRKLMTFAIVLCAFGSA
                     FFASTDSVFTACIGRFLIGIGSAFSFIGVLVLISRWFPPYYFAILAGVAQLMSSVGAM
                     FGEMPLAALIDLVGWRSASFILAGVGFLLAILFWVFIRDYPHQQNQTIPDHYLRDEWK
                     RLVAVCKHAYTWIIGGYAFAIWTPIAVFAALWGVPFLQEKFQVSVVAASGLCSMIWLG
                     IGVGSPVLGWLSDKLESRRIALIISAVLGLIATLIILYWSELSYNWAYVVLFVLGLGA
                     GGQTVSFAVVKENNTPELVGTASGFNNLSVLIGGAIFQPLVGYILQQTGSWRIVNGAH
                     VYSISSYQTALLVMPVCFLASLLIATFLLKESHPARRD"
     gene            complement(15898..17178)
                     /locus_tag="AC06IO_001231"
     CDS             complement(15898..17178)
                     /locus_tag="AC06IO_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874432.1"
                     /translation="MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKS
                     FNAHESVVATSLTVYVLGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHS
                     MNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
                     IIIHYASWRLIFIIIALGALLAYWGLWRFMPEPIGQVKRDGQLIAKTPFALNAITRNY
                     KTLLSNQAFCYSAIAEGLVGIPCIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATIL
                     GNWCLHHLTYKYKIERIIFIGGIIMIVGLALTALLPFFYGNNYVYLIPGIIIYFFSLS
                     VINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNA
                     VGLLFLIFIGLTFFIGTPKKSEIHNEANSNVANS"
     gene            17409..18575
                     /locus_tag="AC06IO_001232"
     CDS             17409..18575
                     /locus_tag="AC06IO_001232"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9874433.1"
                     /translation="MPKEKVVIIIPTYNEEDNISNTIDLILKETYSLEDYQVDILVFD
                     SHSTDSTAHIVRELTYTSQRIHFAQEPQKTGLGSAYLQAMRIAIDELNADVVFEFDAD
                     GSHQPCYIAPMLELLKQHDVVVGSRYISGGRIPSNWAFHRKLLSVGGNWVARMLLTGK
                     YKDLTSGLRAIRTNLLKKLLPSNFLSNNYAYKLELYWLLHKAKASIVEFPIQFIDREK
                     GKSKLPKNSMIDTLRVLFTLRFRELRQYLTMCCVGSIGALIQFSLYNWLRSSNYSALV
                     SSQVAISAAIISNFIFHHSITFKQKTDFRITKTTFSRFTQFIIYSTFMVYFQSLWVHI
                     FVHSFGQGVLKENIIVASGLGIGSIINYLFYSNIIWSSKGTHSLSSNSSKETQI"
     gene            complement(18612..19997)
                     /locus_tag="AC06IO_001233"
     CDS             complement(18612..19997)
                     /locus_tag="AC06IO_001233"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIO9874434.1"
                     /translation="MFLHMNRYISLYFLLILLIAPIHLLGLDSYYYWDWSRHLALSYY
                     DGSPMIAYFIRIATLLFGDTLFALNLVTIVSTALTSFIIYKTARLCLNKEASYVTVLI
                     WLFSPLVTLDLINQTTYDTPLTLFWALTLYFTIRYIQFNRNQDLYYIGLSIGLMLLSK
                     YSGIVLVLALLVFLVATPYRRLFKSRHFYLALTLAIIVFSPVILWNAQHDWVSIRYQL
                     STHQSQNTHHAMGGILHAFFNFFLPAINFLFIPPALYLSKGTPRKSPVFYLCLIISVT
                     FVLFYLFVASKAMIRGYWLSQYIITASLLAGYCYQEWAYRNSTYLLIVFYVLTSGIIL
                     MNNTTLFSFGYSKKLAYYQSIQQINQSNDKLPSIAITPGWFEARMLFFLKNKPTIFTF
                     DCGSMQNQYRYWSPNLNTLGEVLYIDTFDRLSCVKKYFARCQSLNLPAAFDEKGKPII
                     YAYWCSNKLDI"
     gene            20096..21238
                     /locus_tag="AC06IO_001234"
     CDS             20096..21238
                     /locus_tag="AC06IO_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIO9874435.1"
                     /translation="MKLYFFGDDNNKPKLSEHLIIYILSLLDPKSRSVASLVNKDWSR
                     LVQLTRQYLSMLPVIHRIPLFANLGLDVLKLKLMTGGMTNSTFRLRIKNAPEKWVMRI
                     PGIGSSAFITRRDEAHNARQAEKLGLNVPIAFFDPEDGLQVTRFIEGVHFLDDKALAR
                     KEILHEVASMMRRLHKSPPFDNSTLFFERNEELLDLLKRKSFNFPGEIEFIEQQMKNL
                     KEIFSSYHIQQFPCHNDTTPLNFILSENPEIKGSEKIHQIDWEYSSNNDFIWDLVYFM
                     VEAKLNREQQLILLKAYFNTEELSDSLLAWIEVYKPIIEWWITIWSWTQLANGANAVD
                     LSSYEQLGLERYHNTLSHLKTEGYVKALELIEADSHRLTFTGQRLF"
     gene            complement(21291..22061)
                     /locus_tag="AC06IO_001235"
     CDS             complement(21291..22061)
                     /locus_tag="AC06IO_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIO9874436.1"
                     /translation="MTILVFDIETIPDIESGRRLFALDGLSDEDTACAMFALRREKAS
                     NDFLPHYLQKICAISVVISHGNQIKVWSLGDENSDEKELITRFFAGIDKHTPTLVSWN
                     GSGFDLPVLHYRSLYHSISAPTYWETGENQQTFKWNNYLSRFHYRHIDLMDVLAGYQN
                     RAFAPLDDISTMLGFPGKMGMSGSKVWEQYQAGQLKEIRDYCETDVLNTYCVYLRFEL
                     MRGTISQEGYDRNITQLKNYLGAEKDKMHLQEFLKKMI"
     gene            22307..22903
                     /locus_tag="AC06IO_001236"
     CDS             22307..22903
                     /locus_tag="AC06IO_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874437.1"
                     /translation="MKIRFTLLCFSIFLTGCVSKPPADINNICNIFKQYPKWYVDSKD
                     VERRWKVPVPVQMAIIHQESKFNARARPPRTKLLWIIPWTRPSTAYGYTQALRSTWDN
                     YKRSNGWFWSSRDDFGDGVDFIGWYANQANIKAGIPRTDPYSLYLAYHEGIGGYQRKT
                     YLKKPWLISVARKVKAKSQLYAMQLNSCQGSLKSRSWW"
BASE COUNT         7179 a         3942 c         4735 g         7145 t
ORIGIN      
        1 cacctattca tttatactca ttaattttag aagaagtagc gttatgattg aagtaaatat
       61 ttggttatca actgcttttt tgtttaagaa acgaattaag cacaaatttt ttggtccgct
      121 tttagcttca gaggacaaag gggaaaatgt agggcatgtc aattttacta tagaaattga
      181 tgagcggaca aaaaatagtt ttgattttat tgaaaaacac gggcctgagc tgggggctaa
      241 aaaaacattg cgtgccgtcc caactcaagc gactgaacct tccatgacaa acatagaatc
      301 gtctcatttg aaaccaacta tggtcaagag tgatgtggtg tctcatagct tttggccaga
      361 agggaggcca agtaaatcag aaactataaa aggtaaagga gtcaaaccca agtttaaaac
      421 tcatgaagac gatatgattg ctgaagactc agtaacggca atggatataa cacatcgcaa
      481 atctgcttta gaagatattg atagggaaaa aaagaaaaat cttgacttgc tggttgaaat
      541 ttcagatttg gaagtcaaca tggagcaaag atcacttttt acggatgatc ttaagaaatt
      601 gcatttggaa aaagaggatc tgagtaagca acagaagcaa ttaaccaatt cccaccaaac
      661 gcagttaaaa gatttaaagg ggcaattagc caagctggaa cttcatttga ataaaacaag
      721 cggacaaatt aaagctacag agagaacttt agcctatttg aacaaattgg aaaatcccga
      781 tcctaaaacc agagcacaat cgattgagct tactgaaaaa ttaattaaat taaaaaaaga
      841 gttcgaaact acaataagtt ctcaacatga gataagcgaa ttgatgtcaa aatctgaact
      901 cacttatcat gaaggtatgc aaaaaattga aaaaaatctt caacaggttg agcttgcaat
      961 taactaccgt gaagaaaatc taaggcaatt ggatattaaa atcaatggca gggatgaaaa
     1021 tgatttaaag gaattacaag aagaggctcg ccatcgcaaa gaatatattt ctcgtaaaga
     1081 acaattcgtc aagagtcgag attttaccaa aggtagacat atcctgatta cagtgtcaca
     1141 ttgccaactg cggaaagtgg ttttacttat tatgttgatg aagtcaaaat actgaaagct
     1201 atgcaagaag aacgcagcca aaattactca tttcttttta ataactgtgc cagtagtgcg
     1261 aaacgttgtt tgctagcagg aatagatgaa aaattgcgag ccaaattaaa agaagctggt
     1321 ttagatagca agttttttga aattagtaaa gtggaaacat gcaagtcatt acgtgactgg
     1381 gcaagaactc ttgagagtaa attaacggag cttaattttc caccacccgc acctacccga
     1441 ggtgcctaag aataataagc agttcatccg taaaaaacta agatcactgc ccaagtttta
     1501 ggcagtgatt tttttataaa tcatcttcat caccaatgct aaacgaagag ccgcaaccac
     1561 aagtcgtttt tgcattagga ttgcgtatga caaattgctc tccttgaatt cctttaatgt
     1621 aatcaatttc cgcatcatgc agatattggt aactcattga atcaattagc aatttgacag
     1681 aagattttcc atcagaacat tgttgtatga taaccgtgtc gtcatcattg atttgttcat
     1741 caaagctaaa tccatattga aatccagaac agccaccacc tgtaattgag acgcgtaaat
     1801 tcaaatcgga attatcttct tctttaatta attctgctac tttatcagct gcgctgacag
     1861 aaaaatggat atctgaagtg ttttgggata cgtcaacact ggccatactt agctccaaaa
     1921 taattactac cattattatt cattatatca ataatgtgag ttgtttcgta gtctcaatcc
     1981 ataaattggt atggggacca gagaaaacaa acagtacccg tacaaaattt gtttccctgt
     2041 ttagcgaata atctgctcta tagtagctcc acccaagcat tgattttttt cataaaagac
     2101 gatgaattgt cccggagtaa tcgcacgttg aggacttgaa aacatcacat agtgttgatt
     2161 attatccgga ggagaaatca tgcaggcttg atcggtttgc ctatatcttg ttttggcaaa
     2221 gcaggttagt ggtaaatgat tctcatagtc tgccagccaa tgaatgggac cacatatcaa
     2281 accttgagaa tagagcattg gatgttgact accctgtgct acgtaaaggg tgttactggc
     2341 tatgtcttta tccacgacat accatggctc atctgtcgag ttttgtaatc ctccaattcc
     2401 caaaccctgg cgttgtccta atgtgtaaaa cattaaacca tcatgttgac caagtgtttt
     2461 tcctccagta ctttttattt ctcccggttt ggcaagtatg aattcattaa ggaaagtttt
     2521 gaatcttttt tcacctataa agcaaattcc tgtagaatct tttttggcat gagtgacaag
     2581 gccaagttgt ttagcgaatt ctctaatttg tggcttggta aaatcaccta ttggaaaaat
     2641 ggttttggac aatgcctctg gttcaacagc atggaggaaa tacgtttgat ccttttccct
     2701 atcttttgcc ttaaataaat agccaataga tccttctatt gtatttttgg catagtggcc
     2761 agtagctata taatctgcgc caagggttaa agcatgattc aaaaaggcat taaatttgat
     2821 ttctttatta cataacacgt cagggtttgg tgttcttccc ttttcatatt cactcagaaa
     2881 ataagcaaat actctatccc agtattcttt tgaaaaattt acagtatgta gtggtatgcg
     2941 caattgatta cagacagcct gggcatctgc caaatctttt gctgcagggc aataatcatt
     3001 gtgatcgtcc tgctcccagt ttttcatgaa tagtccttcc acctggtatc cttgttcttt
     3061 caatagccag gctgctacag aagagtctac tcctcctgac ataccaacaa taactttagc
     3121 tttcataaat ggtttgtatc gctctttttt gaatcataat catattggaa tgtgcgattt
     3181 taacaaatta actacactct aggaaattgt aatttagccc tgtatttatt acccaataag
     3241 ttgatatttt ttacatataa tcggataata ccacgttttt agggattcgt tatcatttta
     3301 acttgattat gttagtaagt cataaaccgt ctataagtgc ttatataaac ttgcaagact
     3361 caaagagcaa actcattcta tatttcggat aactatctat gatgcattta caagagttgg
     3421 caaaacatgg ccagcaagaa aaggaagtga ttttaaacga gcgtctaccc tcgtttgtta
     3481 gtcccggatg ccgtttgcaa gcgtcttatc atatagaggc aaaagatgat ttttatctta
     3541 ttcatcttag agtgcagggc aatttaaaca gcatatgtca gcgatgtatg gaaaacttta
     3601 ctgtatgtta taataactcg actgttattg ctgtttgccg aaatgatgag agagctgagc
     3661 aacttttgag tcaatatgag tgcttggttt cttcaaactg gcaagttgat ttaaatgaat
     3721 taataattga tgagctgcat ttgtatgcac cacaagttca tcctgatatt aatgaatgtg
     3781 ctaaggaaat caatgaaatt ttaactggga aaaaacaaac ttcttgatat tttgataatt
     3841 aatacttgga ttgaagttaa aatatcggta atattcccgc tttatgaatg caatttgttt
     3901 aggagtaata caatggccgt acaacaaaat aaaaaatcac gctcacgtcg tgatatgagg
     3961 cgttctcatg atgcactaac caagcctact ctatcagtag atccaacaac tggtgaaacc
     4021 catcttcgtc atcatatgac cccagatgga tattacagag gcaaaaaaat tattgacgct
     4081 gaaaccgctt acgaacaaga gtaactttct tgaagaatat caccattgca attgacgcga
     4141 tgggtgggga tcacggcttg gaaattgtga ttcctgcctg tattcgtgct attaaaaaca
     4201 accctgattt gaaactattg cttgtaggag ttcaggacaa gatcagtgct tctttgaaaa
     4261 aacatggcat gttgtcctgt cagcaattta ctatcgtcca cgcatcagaa gtggttacaa
     4321 tggatgaatt gccatctcat gcattacgaa ataaaaaaga ttcctcaatg cgaatagcaa
     4381 ttaacctggt taaggaaggc agagcacagg cttgcgtcag tgcgggcaat acaggtgcct
     4441 taatggctac cgcccgatat gtcttgaaga ctttaccggg gattgatcgc ccagcaatag
     4501 tatccgaatt acctaccatg ggtggaaaaa caagagtcat tgatttggga gccaatgttg
     4561 attcttgcgc agagcatttg tttcagtttg ctgtgatggg gtctgcattg atacaggcaa
     4621 tagaaaataa acccaaacct aaaattggtt tacttaatat tggcgttgaa gaaatcaaag
     4681 gtaacgatca ggttaagcgt accgcacata tgctggctga gtgttctgtt atgaattatg
     4741 ttggatacgt tgagggtgat cacttttaca gtggtgatgt tgatttagtg gtctgtgatg
     4801 gatttgttgg aaatgtggca ttaaaagcaa gtgaaggttt ggcaaagctt ttattaactg
     4861 tccttaaaga atcatttagc agaaattggt taacaaaaat tgttggtttg attgctttac
     4921 cagccttaaa acatttaaaa aatcgtttgg atccatcgcg atacaatggt gccagtctac
     4981 tcgggttgaa tggtatcgtt gtcaaaagtc atggtggagc caatgaagtg ggttttcaac
     5041 atgctattga acaggctgtt cttgaagtaa aaaataatgt tgtcgattta gtgcgtgatc
     5101 aaataaatga ttttataaat caaggattgt tgttatgaaa aatgctgtta ttagtggcac
     5161 tggaagttac tctccagaga gacaaatgac taatgctgaa ctggaaacca tgcttgatac
     5221 tagcgatgaa tggatagtta ccaggactgg tattagtagt cgtagtgttg ctcaagaaca
     5281 tgaaacaaca tcttatatgg cctccagagc agcagagcaa gcactagagg catcaggcct
     5341 tgatgctgaa gaaattgatt tgatattagt agcaacatgt accccggatt atttttttcc
     5401 tagcgttgcc tgtcacgtac agcatgcttt aggaatcaaa agacctattc cggcttttga
     5461 cattggagct gcatgcagcg gttttgttta tgcgatggat gtagcgaaac aatacattgc
     5521 tacaggggct gccaaacacg ttcttgtcgt aggcagcgag agcatgtcaa gagcggtaga
     5581 ttggactgat cgttctattt gtgtcttatt cggagatggc gcaggcgctg ttgttttaag
     5641 cgcaagtgat cgccaaggga ttatgggtag tgttttacat tctgcctatg actctgataa
     5701 attactagtc cttcgtaatt caacttttga acaagatcgt gcaacgattg gaatgcgagg
     5761 taatgaggta tttaaaattg ctgttaatat tatgggtaat attgttgatg aagtgttaga
     5821 agcaagtcat ttaaaaaaat ctgatattga ttggctgata cctcatcaag ccaatatacg
     5881 catcatacaa gccatagcta aaaaattatc tcttcctatg tcacatgtta ttgttacaat
     5941 tggtaaccaa ggtaacacat cggctgcttc tattccctta gcacttgatt attctattaa
     6001 aaataatcag attaaaaggg atgaaatatt attaattgaa tcctttggtg gtggaatgac
     6061 ctggggcgct atggttattc gttactaaat taactaataa ggattaattc tattatggta
     6121 aaatcagcat ttgtatttcc tggacaggga tcgcagtcta tcggcatgtt atctgacttt
     6181 atgccacagt atactgttgt caccgatgct tttgcagagg cctcagatgc tttgggctat
     6241 gatttgtggg atttggttca aaatgggcca gagacaaagc taaatcagac tgaatacacg
     6301 caggctgcca tgttaactgc tgatgttgca atttatcgtt tattaatgca attaggcgta
     6361 cctcagcctc aagtaatggc tggtcatagt ttgggggaat acgctgcttt ggtttgcgct
     6421 aattctttat ctttaaagga tgctgcacag ttagttgctc gtagaggaca ggtgatgcaa
     6481 aacgcgatcc ctctgggaga gggggctatg gctgccatag taggcttatc tgatgaacag
     6541 gttcgttcat tgtgtgagca ggccagcaca gccaatcatc ttgttacccc ggcgaattat
     6601 aatgccctgg gccaaattgt tgttgctgga catcgagtag ccgttgataa actaatacaa
     6661 ttggctgaag aggctggcgc cagactggca atgataattc ctgtaagtgt gccttgtcat
     6721 tgccccttat taaaaaatgc tgccgaatta tttgctgaag atcttgctca aattgaattt
     6781 caagttcctt cagtcgatgt aataagcaat gttgatttaa gtatttatca ttccacccaa
     6841 catataagag ataaactcaa agagcaacta tacagccctg tgcgctgggt tgaaacagtt
     6901 cagttaattc aacagcgcgg aattgatctg gttgtggagt gtgggccagg aaaagttctg
     6961 aacggtttga taaaaagaat tgagagaaat ttaactacga ttagcgttta tgacaccatt
     7021 agtcttgatc aagtcataga gcgtttaaat atcccagttt gagaggaaac tagatgatca
     7081 atttagaagg taaaattgca ttagttactg gtgccagccg tggcataggt cgtgctgttg
     7141 ccgagaaatt ggcaagaaat ggtgcctttg tttttggaac agcaaccact gaacagggtg
     7201 ctgcgtctat taattctttt tttgaaaaag aaaaattggc tggtaaagga atcgttttgg
     7261 atgtaaccag cactgagcaa gtagaaaatt tgatgtctac cttgtctgat aatgatcagt
     7321 gtccttctat tcttgttaat aatgctggca ttaccgcaga taacctgtta ttgcgtatgg
     7381 atgatgagga atggtacaaa gtaatagaaa caaatttgaa ttctatttac agaatgacta
     7441 aagcttgctt aaaacctatg ttcagagcgc gttggggaag aataatttct ataggctctg
     7501 tggtaggtgc cagtggcaat tctggacagg taaactatac tgcagccaag gctggagtta
     7561 ttggtttttc caagtctttg gcacaggaaa ttggtagtag aggaataact gtaaacgtgg
     7621 tagcccccgg gtttatcgac acagatatga ctactgcatt acctgatatg gtgaaagagg
     7681 aaatgttaaa aagaattccc atgcgtaagc tgggccagcc agaagatgtt gctgatgctg
     7741 tagcttttct agcatcagac agcgcaaaat atatcacagg tgaaacaatt catgtcaatg
     7801 gcggtatgta tatgagttaa atgcacttgc gttatctgta taattgaata aactagccgt
     7861 cagtatttaa taatttctat caaccgaaag aggaaaatta cgttatgagt acagttgaag
     7921 agcgtgttcg caaaattgtt gttgaacaac tgggtgttaa agaagaagaa ttaaagaatg
     7981 atgcatcctt tgttgatgat ttgggtgctg attccctgga tacagtagaa ttggttatgg
     8041 ctcttgaaga agaatttgaa acagaaattc ctgatgaaaa agctgagaaa attactacaa
     8101 ttcaagaagc aattgattat attgaatcta acttaaataa agaagaagct taattttttt
     8161 aaggagctct cattgaatag tcggcgtgtg gttattaccg gcatgggtat gcttactcct
     8221 gtcggactta atgtagaaga aacttggcga agtattttag ctggagttag tggggtaggt
     8281 ttggtggaag attttgacac tactgaatac ccaactaaga tttgggctaa ggtcaagaat
     8341 tttaatgttg agaatcacat gccaattaaa gacgcccgca aaatggacgt ctttacacaa
     8401 tacggtatgg ctgctgctga tgaagcgatg cttgactcgg gtttaaaaat tgatgaagtc
     8461 ttatctcgtc gaatcggtgt cgcagtgggg gcaggtattg gtggtattca aacaatcaca
     8521 aataaccagg ataaactggt tgccggtgga ccacgtaagg tttcgccatt ttttattccg
     8581 gcaggtatta tcaatatggt tgctggacag atttctatta aacataattt aaaaggacct
     8641 aatatttcgg ttgtgactgc atgtactacc ggaacacata atatcgggct agccgggcgt
     8701 ataattgctt atggcgatgc cgatgtcatg atttgtggtg gggcggaaat gaccacaacg
     8761 ccactttgtc ttgctgggtt ttcagcagta agatctttat ccaaacggaa tgatgagcct
     8821 gagaaagcct caaggccttg ggataaagac agagacggtt ttgttatggg tgagggtgct
     8881 ggtattttgg ttttagaaga atatgaacac gctaaagctc gtggagccaa aatttatgct
     8941 gaattggttg gttttggtat gtcaggagat gcttatcata ttactgcacc cgatgaagat
     9001 gccgatggag cttcacgagc tatggaggca gccatccagg atgctggtat cgatcccaga
     9061 caagttgatt atattaatgc tcacggtacg tcgacttatt taaacgattt aaatgaaaca
     9121 aaagccatta agcgtgtttt ccagaaccat gcttatgatc tggcggttag ctcaaccaaa
     9181 tccatgacag gccatttgct cggagctgca ggtgcagtag aggcaattat aagtatttta
     9241 gccatcagag atcaggtggc tccacctaca ataaatttgg ataatcctga tgaaggttgt
     9301 gacttgaatt atgtagcaca tgtaccacaa agcagaacaa ttaattatgt tctaagtaat
     9361 tcacttggtt ttggtgggac aaacggtagc ttactgttca agcgaatcta aatgcaattc
     9421 tctagacata aaaaactggt tattcttgtt ttatccttgt tttttatgtc tttcttgttt
     9481 ttattttgga atatatatcg cttattgtat acaccaataa ttccgaaaac tgcttctcca
     9541 atgattattg ccttggataa ggcgacgtcc gcttatcaat ttgcttatat tttgcaagat
     9601 aaaaaattaa tacattctgc caaatttttt ctattgatta ttcgttttga aggattatcg
     9661 catcagttaa aggcaggtgt gtatcaaatt actcctggcg aaactgcaat gaaattacta
     9721 catcgagtag ttgctggtga tgtaattacg caaaatttta cgatcatcga aggatctaca
     9781 cagcagaaag tagattatga cctaagacaa gctaattatt tgaaatacaa tccagaagat
     9841 tgggcaatag taaaagaaaa ttaccaaagt gcagaggggt tacttctagc agacacttat
     9901 caataccaag gtgggagtag tagtagggca ttattagagc aagcccatcg taatttatta
     9961 aattatctta atactagttg gacaaacaga gcaccaaatt tgccttataa aactgcttat
    10021 gagctattga ttgccgcgtc aattattgaa aaagaaactg caattgcgca agagaaaaaa
    10081 ttgatttctg gtgtgatggt aaacaggtta aagaaaaaaa tgccattaca aatggatcct
    10141 acagttatct atggactggg aaatcaatat aaaggaaaat taacacataa tgatttgcta
    10201 attcaatcac catataactc ttatttgaac agagggttgc ctcccacccc aatagctatg
    10261 gttggcaaag aggccataga tgccgctgca catccgcaat tgtccaatta tttatacttt
    10321 gttgccaaag gagatggcac tcatcaattc tccgaaactt atgagcaaca aagacaagca
    10381 attaaccaat acagacgcaa ggattattaa tgtcatcgtt aaccgggaaa ttgatagtta
    10441 ttgaaggttt ggagggagca ggaaaatcta cagctgtcaa tttagttgtt gaactgttat
    10501 cccaaaaaaa aatcagtact ataaccacga gagaacccgg aggaacaaaa ataggtgaaa
    10561 ttttaagaag tattattaaa aatccagagt acaataatgt tctggatgac agaagcgaat
    10621 tacttctcct gtatgccgca agaatacaac tgattgagca agtgatcaaa cctgctttaa
    10681 atgtggggca gtgggtcata gctgatcgct ttgaactttc tactttagct tatcagggag
    10741 ggggccgcaa aatggatatg cgggttatta atgagctatc gaatttctgt ttaaaaggat
    10801 tcaaacctga tctcacttta tatttagaca ttaatcccga gcttggcatg ataagagcca
    10861 aatcaagggg aaaatttgac aggattgagc aggaatcaat tgagtttttt catagaatac
    10921 atgatacata tcatattctt gttaaacaaa acccggaaat tatgatgatt gatgctaaca
    10981 ggtctttgga agaggttcaa tcttccattc aatcagtaat cgaggagttt atagagcata
    11041 acctatgatt acttatcaag aacaatggca gaaaatgcaa atggccttgg cgataagacg
    11101 aattcctcaa gccatgctat ttttgggttc tttgcattgt ggtcttatag aatttacaaa
    11161 tcaagtgatc aaattactct tgtgtaaaat aaaacagaat gagccttgtt tgacttgcat
    11221 ggattgccaa atgatagagc gttttgagca tccggatgtg gaatgggtta agcctgaaaa
    11281 aagcggtggt attattaaaa ttgatcaaat taggggattg cagagtacgg cgtttctaac
    11341 accacagagg actaatcata aacttataat tatcgaatca gctgaatcaa tgaatattgc
    11401 ggcatcaaat gccttattga aaattctgga agaaccagcg caacatactg tctttatttt
    11461 gttagcgcaa caaattggta atataccacc caccgtactg agccgatgcc aaatgacgca
    11521 atttgctgtt tttgatgagt tgccttgcaa caatttattg gctttaggag agtactatac
    11581 tcaggaatcg aatagcacaa tggtattcaa gcattcagaa tccattttag aaggcttgat
    11641 tgcaattatt gagcgaagag agcatccatg cactttggct gcacaatgga ttcaacatga
    11701 attaagtgct tttttgtggt ttttatattt gctctattct caactgcagt atatgcattt
    11761 gtttcgagtg gcttattctg gtacgttaac aaaacaattg aataagctat cttcattact
    11821 caatccggtt atgatttttg cccaagtaga taaaatcaat actttgttaa gaaaattaag
    11881 ccataatatg aatatgaatc atacattggt cttggaagat ttattatttt ctttggttgt
    11941 tgattattaa ttgagtttaa aggaattaaa ccattgcgtg tgaaccgagt tttttattaa
    12001 gcaactgaag tgagcaagat gattagttaa aacaggacta ttcaaggagg atgtctattg
    12061 attgaacgga ggagttatga ctgaaaaacc acagcttata aactgctcat ttcccaatga
    12121 agcatctttg tatatggctt acatgccttt tgtgaaaggt ggcggtcttt ttataagaac
    12181 tcaacacgtt tattctctag ggcagtcgct tgaattatct gttaaattgc tcaatgaaaa
    12241 tgaaccatat ttggtcactg gtaaaatagt ctggattact cctaaaggtg cacaaggtaa
    12301 taagccgccg ggagttggta ttcaatttac cggtgaaaac agtcgttatc tatgtaacaa
    12361 gatagaaact tatttggcag gaatgctaaa atcatgtcag gttacagata ctatatagaa
    12421 ttttcattta ttcatgaaca atatattcat tgatattatt ttggggtata aaggtgctgg
    12481 tcgactctca ttgtcattta aattttatcg atttgactca ttttaatcat gatcttgctc
    12541 aagtaatcaa tcaggctcgc gagaatgggg tggagcattt cttgtctgtt tgtgttgagt
    12601 tgagcgatta ttctcaatta gagcgtttag ctaaggatta tcctgatatt agcatttccg
    12661 ttggagtgca tccgaatagt gaaatgaatt atcctgttac ggcacagatg ttatgtgaat
    12721 tggctcaaaa tcctgcctgt attgccatag gagaaacagg gcttgattat tatcgtaatc
    12781 ccactgagga agctcaatct ttgcaacgag atcgtttcag agaacatatc aaggcggcac
    12841 ttataacttc caagccgtta attattcata ccaggcaagc tgctgaagat actttgttga
    12901 tgatggctaa ggaaggggct agtcagatag gtggggtcat gcactgcttt gctgaagact
    12961 tggatattgc gcaaagagca attgatttaa atttttatat atcattttcc gggatagtta
    13021 cttttaaaaa cgctgtttca ttgcaagatg tagccaggca gataccttta gagaggatac
    13081 tgatagagac agattcacct tatttggctc cggttccaca cagaggaaag caaaatcatc
    13141 ccgctttggt gaagcacgta gcagaagcta tcgcacaact cagagggatg gatttccaag
    13201 agatagcaga aatcactaca aacaattttt ataattgttt taatatcaaa cgacaaggat
    13261 aaatcattgg taaatcgtag ttctcaactt caaaacagaa gcggtaattt ttttgcctgt
    13321 gaataaaggt tttagtagta ttgatatatc ctattgcaaa tttagagtaa ttaatgagtt
    13381 tatatatcgg tttgatgtcc ggtaccagca tggatggcat tgatgctgct ttgttggagc
    13441 ttccttccaa tcaattgatt cacgggatta ctaaacaata cagtgatgat gtcaggagaa
    13501 atcttgatga tttaattatg ggtaatcatt taaccctggc ttcgatttgc caactgaaca
    13561 cgctaattgg cagagagttt gcggaagcag ttcgccaact cttgattgaa ataaaagtgc
    13621 atccaaaaga aattcaggcc attggtagtc atggtcaaac tgtctgccat gatacaagtg
    13681 gaagtatccc gtatactttg cagcttggct gtggccatac gatttcttct ttaactggca
    13741 ttaccgttgt agcagatttt agaacaagag atttggtgaa tggtggtcaa ggtgcgcctt
    13801 ttgcccccct ttaccatcaa cagatatttt ctaaagtgaa tgagtctgtt gctgttgtta
    13861 acattggtgg tattgctaat gttaccttta ttgcaaagaa tcaaatgact agaggttggg
    13921 atatagggcc tggcaattgt ctgatggatg cctggattta taaaaataaa ggagcccttt
    13981 ttgataaaag tgggatttgg gcgagccaag gtgaagtgat tcatcctctg cttgaatatt
    14041 tgttacaaga tccatttttc catttggatt cacctaaaag cattggcaaa gaatattttt
    14101 ctttgtcctg gctacaaaaa catttaaaac cggattacac accagctgat attcaggcta
    14161 ctttgttagc gcttacagcc catacgatag ctgaaacaat attaaatgaa tcaggagaaa
    14221 ttaaacaatt atatctctgt ggaggtgggg cccataatac gcatttgaaa gagaatttag
    14281 ctcgtctact gcctgggata gcagtaaaaa gcatagcaga gttagggatt agtcctgatt
    14341 atcttgaggc aatgatgttt gcatggttag cagcacagac gattaatcaa ataccagtta
    14401 atttaacatc aataactgga gctaaaggta ttgcaatttt aggtgcagtg tatcctataa
    14461 taaaatcata ttgacaaact aaagaatcat aaggtttaat ttcataaaaa ttaacagagt
    14521 gattttaaat tgaccgcaca ttacaacgtc gcttcaaaca gtggagcaag aaatacactt
    14581 gcaacgtctt attttatttt ttttctagcc gcttcatttt acttgtatga attcatacta
    14641 caagtagcac caagcgttat ggcagagtcg atgatgaaaa cttttggggt gacgggtcaa
    14701 ggctttggtg tcatttcagc tttttatttt tatgcttatg cccctacaca attgccagca
    14761 ggagtattat acgatagata tggaccacgc aagttaatga cttttgcaat cgttttgtgt
    14821 gcatttggtt ctgccttttt tgcttccact gacagtgtat ttacagcttg cataggccga
    14881 tttttgatcg gaataggctc tgcgttttct tttatcggtg ttttggtcct tatttcacgc
    14941 tggtttcccc cttattattt tgcaattcta gcaggggtag cgcagttgat gagttcagtc
    15001 ggcgctatgt ttggtgaaat gccattggca gctttaattg atctggttgg atggagaagt
    15061 gcgagtttta ttttagcggg tgttggattt ctcctggcta ttttattttg ggtatttatt
    15121 cgagattacc ctcatcagca aaatcagaca atccctgatc attatttaag agatgaatgg
    15181 aagcgcttag ttgctgtctg taaacatgct tatacatgga ttatcggtgg ttatgctttt
    15241 gcaatttgga ctcctattgc agtatttgct gcactttggg gggttccttt tttacaggaa
    15301 aaatttcaag taagcgtagt ggctgcctca ggcttatgca gtatgatttg gctaggcatt
    15361 ggtgttggta gccccgtgtt aggttggttg agtgataaat tggagagtcg ccgtattgca
    15421 ttgattatca gtgcggtttt aggactgatt gcaactctga ttatccttta ttggtctgag
    15481 ttatcttata attgggctta tgttgtcttg ttcgttttag gattaggcgc tggaggacag
    15541 acggtaagtt ttgcagtagt taaagaaaat aataccccgg aattagttgg cacagcatct
    15601 ggttttaata atttgtctgt attaattggt ggggccattt tccagcccct tgttggttat
    15661 atcctgcaac agacaggatc atggcgaatt gtcaatggtg ctcatgtata tagtatatcg
    15721 agttatcaga ctgcgttgct tgttatgcca gtatgcttcc tggccagttt gcttattgcc
    15781 acttttttgc ttaaggaatc gcatcctgcg cgcagagatt aataacagat ttatcaattc
    15841 aattttgcgt tattgaccta aaacaaagga atcagtttat gtcaatgtgg cttgttctca
    15901 tgagttagcc acattactat ttgcttcatt atgaatttca gatttcttgg gagtccctat
    15961 aaaaaaagtt aaaccgataa agattagaaa taataatcca acggcattac aatagagggc
    16021 gaaatgcaga ttattatgat gttgataaaa caggttagct atttctattc caatagcccc
    16081 aatgatcata atacttaggc tgattaatgc cgaggctgtt cctttgctta cagcggttac
    16141 aaataaacaa tacctgttca agggggcatt aattacactc agtgagaaaa agtaaattat
    16201 gattcccggg atcaaataca cataattatt gccataaaaa aaagggagaa gagctgtcag
    16261 agcaagtccc acgatcatta tgataccacc tataaaaata attctctcga ttttatattt
    16321 gtaagttaaa tgatgtaagc accagtttcc taaaattgtt gccccgaaga tgggtagttg
    16381 ccaaaggcca tattgaatta cagttaattt tgcttcagca attaatatga ttggcgctaa
    16441 agcaatccag gcgatgcatg ggatccctac cagaccttca gcaattgcac tataacaaaa
    16501 ggcctgatta gataataacg ttttataatt tcgggttata gcgtttagtg cgaacggtgt
    16561 tttagctata agctggccgt ctctttttac ctggcctatg ggttctggca taaaccgcca
    16621 taatccccaa taagctaaaa gagcacccaa agcaataata ataaaaatca aacgccagga
    16681 cgcataatga atgattatgg ctcctaatag tggccctaat aatggggcta atattgcggc
    16741 attagccatg atagcaatta atcggatagc gtccatttct tcaaatattt cttgaattgt
    16801 ggcataacct atcacaccaa tgaaacacaa tcccattccc tgaaaaaacc gggcgattaa
    16861 aaattgattc atggagtgtg agcttgcaat caaaagagtg aacagaaaaa ataagcatga
    16921 tcctattaac atcattggtc ttctgccata agcatctgat agtgggccaa gtatgagctg
    16981 caaactggcc cctccgagga catagacagt tagagaggtg gcaacaacac tttcatgcgc
    17041 gttgaatgat ttaacaacat tgatcatacc tggcatgatc atgtcattgg ctatataagt
    17101 caaaaattca tataaaacca ggaagcaagc aaagataatg gcctgtctgt atgaaatttt
    17161 aattagtggc tcagacatag cattaaccct gaatcaatcg tttctaaaag gcggtctatc
    17221 agtatatacg tttatttcat aatttggtag aaaatctgat aaatgttttg gtaggcgaca
    17281 taaaatatgc catatactga gaataaatct ttacaatagt aactgccact taaagtttgt
    17341 ttacggcaag tccaccattt gataagattt gcttacttct ataatctgct tcttatgatc
    17401 tgatcagtat gcccaaagaa aaagtcgtta ttattatccc tacctacaat gaagaagata
    17461 atatttctaa tacgattgat ttgattctta aggagactta ctcactcgaa gattatcagg
    17521 ttgacattct tgtatttgat agtcactcca cagattctac agcccatata gttcgagagc
    17581 taacttatac ctctcaacgt attcattttg cacaagaacc acagaaaact ggacttggtt
    17641 ccgcttacct gcaagctatg agaattgcaa tagacgagtt aaatgcagat gttgtatttg
    17701 agtttgatgc agatggttct catcaacctt gttatattgc tccaatgtta gagctactta
    17761 aacagcatga tgtagttgtg gggagccgat atatttcagg gggaaggatt ccctcaaact
    17821 gggcattcca tcgtaaatta ttatctgtgg gaggtaattg ggttgctcgt atgttattaa
    17881 ctggaaaata taaagatctt acttctggct taagagcaat acgtaccaat ctgttaaaga
    17941 aactattgcc ttcaaacttc ctatccaata attacgcgta taaacttgag ttatattggc
    18001 ttttacacaa ggcaaaagcc agcatcgttg aatttcctat tcaatttatt gatagagaaa
    18061 aagggaaaag taaattacct aagaacagta tgattgatac tttgcgtgtt ctttttaccc
    18121 ttcgcttcag agaattacgc cagtacttaa ctatgtgctg tgtgggctcc atcggtgcat
    18181 tgattcaatt cagcttatat aattggttgc gcagttcgaa ctattccgct ctggtatcca
    18241 gccaagttgc tatcagtgct gctattatat ccaattttat ttttcaccat tccattacat
    18301 tcaaacaaaa aactgacttc cggataacta aaacaacgtt tagccgtttt acacaattta
    18361 ttatttattc cacatttatg gtttattttc aaagcctatg ggttcatatt tttgtccatt
    18421 cctttggaca aggtgttttg aaagaaaata tcattgtagc ctcaggcttg ggcattggat
    18481 ctattatcaa ttacctgttt tattcaaata ttatttggtc atcaaaaggt actcattccc
    18541 tcagttcaaa ctcttctaaa gaaactcaaa tttgaaagca aaagagattg ggggtataag
    18601 tcctgtttca tttaaatgtc cagcttatta ctgcaccaat aggcgtaaat gattggctta
    18661 cctttttcat caaaagcagc tggcaaattc aatgattgac accgggcaaa gtatttttta
    18721 acacatgata atctatcaaa tgtatcgata tatagcactt cgccaagggt gttcagatta
    18781 ggtgaccaat acctgtattg attttgcatt gaaccgcagt cgaaggtaaa aattgttggt
    18841 ttatttttaa gaaagaacag catgcgtgcc tcaaaccatc ctggggttat ggctatgctt
    18901 gggagtttat cattagactg attaatttgt tgtatcgatt gataataagc cagttttttt
    18961 gaatacccaa aactaaaaag agtggtattg ttcatcaaaa tgattccact ggtcagaacg
    19021 tagaatacaa ttaataaata agtggaatta cgataagccc actcttgata gcaataacca
    19081 gctaataagc tggcagtaat tatgtattgg ctcaaccaat atcctcttat catggctttg
    19141 ctggcgacaa ataaatagaa aagaacaaat gttacggaaa taatgaggca caaataaaat
    19201 acaggtgatt ttctaggggt gcctttggat agatataacg cagggggaat gaataaaaag
    19261 ttaattgcag ggaggaaaaa attaaaaaag gcatgcaaga taccccccat cgcatgatga
    19321 gtattttgcg attggtgagt acttaactgg tagcgaatgg atacccaatc atgttgagca
    19381 ttccatagaa taaccgggct gaagacaatg attgccaggg ttaatgccaa ataaaaatgc
    19441 ctggatttaa aaagccgcct atagggtgtt gcaaccaaaa aaacaagtaa ggccagcacg
    19501 agaacaattc ctgagtattt ggataaaagc ataaggccta tactcaaacc tatgtaatac
    19561 aaatcctggt ttcgattaaa ctggatatac cttattgtaa agtaaagagt taatgcccaa
    19621 aaaagggtta aaggcgtgtc ataggtagtt tgattgatta aatctaaagt gactaatggt
    19681 gagaataacc aaatcaaaac cgtaacataa ctggcttcct tattaaggca taatcttgct
    19741 gttttataaa ttataaaact ggtcaaggca gtagaaacta tagtaaccag gtttaaagca
    19801 aagagagtat ctccaaataa aagtgtggct atgcggataa agtaggcaat catgggagaa
    19861 ccatcatagt atgatagagc caaatgcctg ctccaatccc agtaataata actatctaat
    19921 ccaaggaggt gaattggtgc aattaataga atgagtagaa aatagaggct aatgtagcga
    19981 ttcatgtgca aaaacattca atattcccag gaaagtaatg atgaattata tgttatttta
    20041 ttatcagttt ttatataatg cattcagttt tatcattaat taataaatga cgtttatgaa
    20101 attatatttt tttggtgatg ataataataa acccaaatta agcgaacatt taatcattta
    20161 catactcagt ttgctagatc ccaagtctcg gtctgttgct tctttagtga ataaagactg
    20221 gtctcggctg gttcaattga cacggcaata cctgtccatg ttaccggtaa ttcatcgaat
    20281 acctttattt gcgaatttgg gtcttgacgt tttgaaatta aaattaatga caggtggtat
    20341 gacgaattcc acatttaggc taagaatcaa aaacgcacct gagaaatggg ttatgcggat
    20401 tcctggaata ggctcttcag catttataac cagaagagat gaagctcaca acgctagaca
    20461 agccgaaaaa cttggactga atgttccaat agctttcttt gatccagaag atgggctgca
    20521 ggtaacacgc tttatagaag gagtacattt tctggatgat aaggccttgg cccgcaagga
    20581 aattctgcat gaggtagcat ctatgatgag aagacttcat aaatctcctc cctttgataa
    20641 tagcacgctt ttttttgagc gaaatgaaga attattggat ttattaaaac gaaaatcctt
    20701 taattttcca ggggaaatag aatttattga gcaacaaatg aaaaatctaa aggagatttt
    20761 ttcttcttat catattcagc aatttccttg ccacaatgat acaacgccac ttaattttat
    20821 cttgtccgaa aatccagaaa tcaagggttc tgaaaagatt catcagattg attgggaata
    20881 ttcaagtaat aatgatttta tttgggattt ggtttatttt atggttgagg ccaagctaaa
    20941 ccgcgaacaa cagcttattc tgttgaaagc ttactttaat acagaagagc tgagtgactc
    21001 tctgttggca tggatagagg tttataaacc catcattgag tggtggataa ccatttggtc
    21061 atggacacaa ttggcaaatg gagctaatgc agttgatttg agttcttatg agcaactagg
    21121 gcttgaacgg tatcataaca ccttaagtca cttgaaaact gagggctatg tcaaagccct
    21181 ggaactgatt gaagcagata gtcatagact tacttttact gggcagagac ttttttagcg
    21241 gtcgccatta tctagagaag acaactcgtt tacagtgacg attaagatct tcagatcatt
    21301 tttttgagaa attcctgtaa atgcatcttg tctttctcag cacctaaata gtttttgagt
    21361 tgtgttatgt ttctgtcata accttcctgg ctaattgtgc cacgcatcag ctcaaatctt
    21421 aaatacacgc aataggtgtt gagtacgtct gtttcacaat aatctcttat ttctttcaat
    21481 tggcctgctt ggtattgttc ccaaacttta gagccactca ttcccatttt tccagggaaa
    21541 cctagcatgg ttgaaatgtc atcaagaggg gcaaatgctc tgttctgata tcctgcaagg
    21601 acgtccatga gatcaatatg tcggtagtga aaacggctta gataattgtt ccatttgaag
    21661 gtttgttgat tttcccctgt ctcccaataa gtgggcgcgc taatactatg gtatagtgat
    21721 cggtaatgca gtacaggaag atcgaaaccg ctcccattcc agctgaccag ggtgggtgta
    21781 tgtttgtcaa ttccagcaaa aaaacgggtt attaattctt tttcatcaga attttcatcg
    21841 ccaagagacc atacttttat ttgatttcca tgactgatta ccactgatat agcgcagatt
    21901 ttttgtaagt aatgaggcaa aaaatcattg cttgctttct cacgccttaa ggcaaacata
    21961 gcgcatgccg tatcttcatc tgataagcca tcaagtgcaa aaagccttcg ccctgactca
    22021 atatctggaa ttgtttcgat atcaaaaaca agtattgtca taatttgttg gtccagttcg
    22081 gtcttaattc ttatttttgc aaatttgctt gaaactatcg acaagtctcg gatttgtcaa
    22141 aatcacaatt gggatatctg attgattcta tcatgtgaag gaatagaagt aactttaaat
    22201 ggcatagatt gtatgcagta tatttgatac aatatttttt aaattgatac cttatatcgt
    22261 agcattgata ttcaatactg caggatgagt tttttatcat taaatcatga agattcgatt
    22321 tactttactt tgttttagca tttttcttac gggctgcgtc agcaaacctc cagcagacat
    22381 taataatatt tgtaatatat ttaaacaata tccaaaatgg tatgtggatt caaaggatgt
    22441 tgagcgacgc tggaaggttc cggttccggt tcaaatggca attattcatc aagaatcaaa
    22501 atttaatgcc cgagccaggc caccaagaac caaactgtta tggataattc catggacaag
    22561 gccatcgaca gcttacggat atactcaggc gttgcgttcg acttgggaca attataaacg
    22621 cagtaatgga tggttttggt catcacgcga cgattttgga gatggcgttg attttatagg
    22681 atggtatgct aaccaggcga atattaaggc aggtattcct cgaacagatc cttactcact
    22741 ttatttggct tatcatgaag ggataggtgg ttatcaaagg aaaacctatt tgaaaaagcc
    22801 atggttgatt tctgtagcgc gcaaagtgaa ggctaaatca caactttatg caatgcagct
    22861 caattcttgt caaggttcgt tgaagtcacg ttcctggtgg taattaagat ggcctaatta
    22921 tgttcttgcc agtgaaaata aggtcagact tggaacatat atgattttgt gtgtttataa
    22981 taatcaccat aacacgaagt c
//
LOCUS       DBNCWK010000034        22895 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.034, whole genome shotgun sequence.
ACCESSION   DBNCWK010000034 DBNCWK010000000
VERSION     DBNCWK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22895
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.034"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(31..810)
                     /locus_tag="AC06IO_001237"
     CDS             complement(31..810)
                     /locus_tag="AC06IO_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIO9874438.1"
                     /translation="MRILSCLVLIISLIACSSKPAPNTLVIGTIAGPETELIETAKQV
                     AEKEYGLNIKIVEFNDYNLPNEALQDGSLDANVYQHLPYLKAAILSHGYDLQAIGRTF
                     VYPMGIYSKKYKTLSELPENGIIAVPNDPSNEMRAFLLLEKAHLITLKNTTNSGIQDI
                     ESNPKQFKFKEIDAAQLPRVLPDVDAAVINTTFALPAGLSPSKDALFTEGKDSPYANI
                     IVIRRDTEKRPQLELFVKALNSEEVKEKAKKLFGEDAIPAW"
     gene            complement(823..1470)
                     /locus_tag="AC06IO_001238"
     CDS             complement(823..1470)
                     /locus_tag="AC06IO_001238"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIO9874439.1"
                     /translation="MSYQMVYDLLNATGETLYMVFFSTLFAVLLGLPLGILLYSSSRI
                     KPNIRLNKMLSALINIFRSIPFIILLVAIIPFTRLIVGTSIGINAAIVPLTVGATPFF
                     ARLVDNVLQSLPLGLIETGYSMGANTRQIILHIILPEAQSGLIHSITVTAITLINYSA
                     MAGAVGAGGLGTLAINYGYQRFNAGIMIATVIVLIILVQLIQMVGDYLAKRCTHY"
     gene            complement(1448..2473)
                     /locus_tag="AC06IO_001239"
     CDS             complement(1448..2473)
                     /locus_tag="AC06IO_001239"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIO9874440.1"
                     /translation="MIELCGLKKSFSGKLALNDINLFIQEGEIFGVIGKSGAGKSTLL
                     RCINILEKPDEGEVVIDGQNLMSLSRKDLALARHKIAMIFQHFNLLNSKTVFDNIALP
                     MRIQGIDEESIKQKIEELLPVVELTDKKDAFPSQLSGGQKQRVAIARALSCSPKVLLC
                     DEATSALDPATTDAILSLLKKINELYGITIVLITHEMDVVKRICQRLSVMVDGKIVET
                     TALSNIFNKPESLARKMLYAQISPELPTCLTRRLADYATEKPLVRLFFQGEEATVPFI
                     SQTSRELNMDINILLANIDRFDGVTCGVLVVELTANPLLLEAFINRCEQAGITVEVLG
                     YVLPDGL"
     gene            2925..3341
                     /locus_tag="AC06IO_001240"
     CDS             2925..3341
                     /locus_tag="AC06IO_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK family protein"
                     /protein_id="HIO9874441.1"
                     /translation="MKKMFLAVFTLCSFTAQAAVELKTFQNIVDAIAEGKRISFVINL
                     KKCTSEMPLNSAIVSVTPNAVMVVGDSRVTASDRHFTLDDPVARGTPMFDYSKFNLDS
                     EGDASIKTTVLNASSYERLGSYQMNCKLGDGFKVFG"
     gene            complement(3511..5451)
                     /locus_tag="AC06IO_001241"
     CDS             complement(3511..5451)
                     /locus_tag="AC06IO_001241"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIO9874442.1"
                     /translation="MNDKVWTPKNPEQTRMWQFMDFAAKKHCQVFENYQDLHTWSVKH
                     PESFWETLCLFFKLNFDTPPHQILNYYSEMIEARWFSGARFNFAQKLLTRRDNHPALI
                     SLDENDCKSVISYAELYEKVAQCAAGLKAVGVTTGDRVAAVMPNTSYTIIAMLATTSL
                     GAIWSSCSPDFGTQAALDRIGQIEPKVLFICDGHQYQGKKHSSSEKVIQLPAAITSLR
                     HVVICPNINEEINLEQLPYASHWDDFIKPVKECDFISLPFDHPVYIMFSSGTTGKPKC
                     IVHGAGGTLIQHLKELGLHSDIKSTDNLCFYTTCGWMMWNWTVSALALGATITLYEGS
                     PTYPTDNRMFRLIDEENVTVFGTSAKFISAIEKAGVKPKSEFQLSELRCILSTGSPLL
                     PKNYDFVYEHIKKDLQLSSISGGTDIISCFALGNPILPVYRGELQCIGLGMEVAVYDE
                     QGHSIKQKRGELVCTKPFPSMPVCFWNDKDRQAYKHAYFERFPGVWAHGDFAEITAHN
                     GLIIHGRSDAILNPGGVRIGTAEIYRQVEKVDDVVDSIVIGQDWQDDVRVVLFVKLRE
                     GKKLDDELINLIKTTIRKNASPRHVPAKILQVPDIPKTLSGKVVELAVRQVVHGQSVS
                     NLQSLSNPQALEYFKDRNELKS"
     gene            complement(5608..6516)
                     /locus_tag="AC06IO_001242"
     CDS             complement(5608..6516)
                     /locus_tag="AC06IO_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIO9874443.1"
                     /translation="MDYPQHVTIIEVGPRDGLQNEPSFLHSDKKIELINLLSQTGLKE
                     IEVTSFVSAKAIPQLADNEEVFQSINKTPSINYSALVPNERGMLKALEMGVQNIAVFT
                     AASELFNQRNINCSIKESIERFKPVLALAKTNQIRVRGYISCVLGCPYEGYIQPSQVV
                     SVTKMLLDLGVHEISLGDTIGVGTPKQTQLLLDAILPVLPITQLAMHFHDTYGQAVAN
                     IYTSLEYGVNRFDSSVAGLGGCPYARGASGNVATEDVLYLMHGLGIDTGIDIFKIVAA
                     GDMICKALGRKNQSKVANAMLANPCS"
     gene            complement(6527..8491)
                     /locus_tag="AC06IO_001243"
     CDS             complement(6527..8491)
                     /locus_tag="AC06IO_001243"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIO9874444.1"
                     /translation="MFNKILIANRGEIACRIIKTAHSMGIQAIAVYSAADRNSLHVRL
                     ADSACYIGEAPAKESYLNIDHIIQAAKESGAQAIHPGYGFLSENPDFAKACEQAGIVF
                     IGPSIKAMEAMASKQLAKQLLEKTKVPLTPGYHGVEQSEEKLLSEAKKIGFPVLIKAA
                     NGGGGKGMRAVHDEKEFHDALAGAKRESMASFADDTMIIERLVLNPRHVEVQIMADNH
                     GNVVHLFERDCSIQRRHQKIIEEAPAPNLSPVLRQRLAEAACEVARSINYRGAGTVEF
                     LVDGEDKFYFMEMNTRLQVEHPVTEMITGLDLVAWQIKIAANDTLPLLQNQIQAQGHA
                     IECRIYAEDPYQGFIPSIGQLQFLKEPSGDGIRIDTGVALSSEITRYYDPMIAKLIAW
                     GHNREEALYRLERSLAHYDIGGVKTNIPFLRAICQHVKFKEAKLSTDFLEKENINLPK
                     PDNQLGMILAISCDYLSMINRTTDPLLQEAFGWQMHLSSHWIWRYQLNSTIIEAQITP
                     IDNKKFKAKIENKEMVIYARYDIDQLIIEIDQKSVKARVENKDHHLIFYTDKGQLSIE
                     RFYWSKLDAQTSAHKGQLTAPMPATVVAILKNIGEQVKAGESLIVLEAMKMEHTIHAP
                     IDGILSDIFYSVGSQVSEGAELLALSESDT"
     gene            complement(8484..9278)
                     /locus_tag="AC06IO_001244"
     CDS             complement(8484..9278)
                     /locus_tag="AC06IO_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIO9874445.1"
                     /translation="MSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMKIRLDSAIND
                     TNVRVIVLKANGKHFSAGADLTWMQSMSNFTEEENLEDSMVLGNLMYSLSQSPKPTIA
                     MVQGAAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPAVISPYVVRAIGERAAKML
                     FMSAEVFDATRAYSLNLVQHCVSDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVAN
                     KKIDEELVHYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV"
     gene            complement(9298..10905)
                     /locus_tag="AC06IO_001245"
     CDS             complement(9298..10905)
                     /locus_tag="AC06IO_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIO9874446.1"
                     /translation="MAKLTTQINTSSQEFKNNQANMQALVTDLREKIHQISLGGDEKA
                     RTKHQQQGKLLPRERLHQLLDPGSPFLELSQLAAYQVYEDSIPAAGIITGIGRVAGNE
                     CVIVVNDATVKGGTYYPLTVKKHLRAQEIALINHLPCIYLVDSGGAFLPLQDQVFADK
                     EHFGRVFYNQAQMSALNIPQIAVVMGSCTAGGAYVPAMADESIMVKNQATIFLGGPPL
                     VKAATGEIISAEELGGAEVHCRHSGVSDHYAENDAHALHLARVAISNLNRKKPDSIHR
                     VDTVPPLYDSEDLTGIIPTDPRKPFDIREIIARVVDGSEFDEFKALFGTTLVCGFARL
                     YGYPIGIIANNGILFSESAQKGSHFIELCCQRKIPLVFLQNITGFMVGSKYEASGIAK
                     HGAKMVTAVANANVPKFTIIVGGSFGAGNYAMCGRAYAPRFLWAWPNARISVMGGEQA
                     ANVLAQITREKYAKQGKEWSLEEEEQFKTQMRSQYETQGNPYYASARLWDDGVIAPQD
                     TRKILGLGLSAALNAPIEDTRFGVFRM"
     gene            complement(10915..11229)
                     /locus_tag="AC06IO_001246"
     CDS             complement(10915..11229)
                     /locus_tag="AC06IO_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874447.1"
                     /translation="MLRHSLIYLLLSILIVVFAKYAHLIVVYIDMFFTYVNLKLTPIF
                     SQTGWGLIIRKILVLMLLPIIIAGIPALIYRALKGKDMPHFIAIVWIIWTIIVLSDIL
                     IR"
     gene            complement(11367..12551)
                     /locus_tag="AC06IO_001247"
     CDS             complement(11367..12551)
                     /locus_tag="AC06IO_001247"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIO9874448.1"
                     /translation="MEHNDVVIVAAKRTPMGAMLGNLSALSSPELGAVAHMAVLSQSG
                     IAPAEIDEVISGCVLQAGIGQAPARQAAIKAGIPNSAGATTINKMCGSGMKAVMFAHD
                     LIKAGTANVVLASGMESMSNAPYLLSKARAGYRLGHGELKDHMFLDGLEDAYDKGQLM
                     GCFAETTAKHFNFSREQQDEYALRSMNRALKAIENGAFREEIAPVTLTTRKGDVTIDV
                     DEGPDAAKLAKITQLKPAFQADGTVTAANSSSISDGAASLILMSASNAEKRGIKPLAK
                     IIAHASHSQAPQWFTTAPVDAIRKVMNKASWKQDDVDLFEINEAFAVVAMAAITQLEL
                     NPEQVNIHGGACALGHPIGASGARILVTLMHALKHQGKKRGIASLCIGGGEATAMAIE
                     LI"
     gene            complement(12563..13723)
                     /locus_tag="AC06IO_001248"
     CDS             complement(12563..13723)
                     /locus_tag="AC06IO_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HIO9874449.1"
                     /translation="MYTGLDFQLGETYDMLRDSVNQFAQTEIAPLAAQIDEKNTFPNH
                     LWRKLGEMGLLGITVSEEYGGANMGYLAHTIAMEEISRASASVGLSYGAHSNLCVNQI
                     FLNGNNEQKQKYLPKLISGEYIGALAMSESNSGSDVVSMQLQARSAGDKYILDGTKMW
                     ITNGPDADVLVVYAKTDKQAGSKGITAFIIEKGMPGFKTAQKLDKLGMRGSNTCELVF
                     DQCEVPSENVLGTVNQGVKVLMSGLDYERTILAAGPVGIMQACMDVVLPYVHERKQFN
                     QAIGEFQFIQGKLADMYTDLNACRAYLYAVAKACDNGRVSRKDAAGVILYTAEKATQM
                     ALQSIQILGGNGYINEYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFKETA"
     gene            14058..14744
                     /locus_tag="AC06IO_001249"
     CDS             14058..14744
                     /locus_tag="AC06IO_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874450.1"
                     /translation="MRKLVLWGHGIEDYREMFDLPQGVENLRLLEYGCGPSAVNYQQT
                     QVKKKGVVSCDPLFVLDKDTLLAKAKMIFANMAEEVRQHQDQFDFSRNGSLDKLLQER
                     QEGMKQFFADYEQGKADGRYLGLTDYHLPFADFTFDFALSSHYFFADLDEQTVDFHLI
                     AIRELARVAKEVRIFPLIDREGNTSQFLGPVLLGLQQENYGVEVREVAFHLHKSGNAM
                     LRVWAQQCVV"
     gene            14792..15790
                     /locus_tag="AC06IO_001250"
     CDS             14792..15790
                     /locus_tag="AC06IO_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874451.1"
                     /translation="MAVSNKKNSEFTPSLRSHMIAALLSMGAAAIILWLASGMTLAAV
                     IVDSFSLGFYIGFMLLMSAAPAVLASPVILPGLLLVVSGLFLGAVAATFAHRLKNNPS
                     LPQNNNGNGLINELTKDKSELLISHDDLQCRSNSLEGENTLSQQNEDNVPGKKFGFMK
                     FWGSLGLTSFASSGVTALLGWLATDFVVSLSIIPVFLLGMTLAIVLVPLSIGLSGIFA
                     LPATTALMFALGGFATGVFAATLAAYVISDNQAQDHPVEPIIRSSMEKQCEDQVSHQK
                     ILNGLKKDKLTANEEVINSDLNEQKSRGPKISKSDTGVKEEHINTAEEYSFCGLRF"
     gene            16076..17077
                     /locus_tag="AC06IO_001251"
     CDS             16076..17077
                     /locus_tag="AC06IO_001251"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIO9874452.1"
                     /translation="MYSLLRPLLFRLDAEKAHSLTLSLLHYLPGFYFRKMAGQPVHAM
                     GLVFPHQVGLAAGLDKNGEHLDALAKLGFSFIELGTVTPKGQTGNPKPRLFRIAEANA
                     IINRMGFNNSGVDVLVENVKSANYKGILGINIGKNKETNLNQAADDYLYCFRKVYDHA
                     SYVTINISSPNTPDLRQLQQGDYFAELLAQLQKEQIKLADQYGRHVPLVVKVSPDETD
                     ETLKQMADIILQYGIEGIIATNTTCSRKMVKNLPCSEEQGGLSGRPLMELSTRCLRLL
                     KQYVGNDVTLIGVGGIDSLESAKDKINAGASLLQVYSGLVYKGPELIHDIVSGLNAV"
     gene            17098..17880
                     /locus_tag="AC06IO_001252"
     CDS             17098..17880
                     /locus_tag="AC06IO_001252"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9874453.1"
                     /translation="MYITKELAVRMEGCIKQSHIEVTKRYSQGKILEINGGAACFSGF
                     ESFLSQVVGWGFKTEPNQYKAEIEAIENFYKSIGHNRVDIELCPFVGNHLINFLSQRG
                     YYITELNNVSVLDLKSYHLVAHSESDGFIIREVKQSELEEWAKRVAIGFGFLEAQEQF
                     YQYASAKGVLAFAAYDHGNLVAGATIAIHGDACDLGVTSTLPLYRGKGLQKKLLFARL
                     NLAKQHGLEFATVTTQPGTVSDLNVQKAGFRCAYTRIKLTME"
     gene            17967..19733
                     /gene="msbA"
                     /locus_tag="AC06IO_001253"
     CDS             17967..19733
                     /gene="msbA"
                     /locus_tag="AC06IO_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HIO9874454.1"
                     /translation="MKNNLPIKSRLLYKRLLSYVKPFWPVLLLGVLANILYSGIDAGF
                     TYMTKLFLDKSFITIDLDFVKQIPLIVLIGITLRGLVSSLGSYCMTWVARSVVKVLRQ
                     TVFSHIIHLPADYYDEATSGQLLSKILYDVEQVAQVSADALTDFIQNICLVIGLLTVM
                     MVICWQLSLMFLLTIPFVGIIVNYTNKRVRRISHKVQKTMGEVTEIASEAIEGYRVVR
                     IFGGERYEITKFNKATEYSRKNDMKVAISKAINVSGVQLVIAIGIAMIIMAAIHLSTV
                     ITISAGSFLAIIAAMLQLIKPMKTLTTLNATIQRGLAGAESVFNLLDLPLERNNGLIL
                     KDKIRGEIEFKHVYHAYRQGQNILHDVNFVIEAGTSVALVGHSGSGKTTIASLLPRFY
                     ELSQGMITLDGMPIQQLSLESLRKQMSLVSQNVTLFNDTLANNIAYGRFDASREQIIT
                     AAKLAYADEFIKQLPDGYDTRVGENGVLLSGGQRQRIAIARAILKDAPILILDEATSA
                     LDSESEHYIQAALEQVMNGRTTLIIAHRLSTIKHAHKIIVMQHGRIVEQGSHQELLDM
                     DGHYAQLYKVQQFGRINEEVVV"
     gene            19733..20704
                     /gene="lpxK"
                     /locus_tag="AC06IO_001254"
     CDS             19733..20704
                     /gene="lpxK"
                     /locus_tag="AC06IO_001254"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIO9874455.1"
                     /translation="MSFFVNRIWYGNHFLQWILVPFSWLYRIVIRTRRWYLQRFCQQL
                     YPIPIIVVGNVTVGGVGKTPLVIEIAKKIQQKGLKVGIVSRGYKAAIKHFPYEVKLND
                     SAELVGDEPLMMARKINCPVVIAPKRNEAVRYLLDKHSVEIIISDDGLQHYKMGRSIE
                     IVVIDGMRKLGNGFCLPAGPLREPDSRLKQVDFVIVNQGAAEGAYSMELIPKNIVRLS
                     TQEEVSNDSFTSEVAAVAGIGNPQRFYSTLSQLGIKFNPYSYPDHHQFKPHDLNDIDL
                     PVIMTEKDAVKCYSFSSDKLYYLPVEAKLNDSFWEAFWSHQQLQGYY"
     gene            20706..21656
                     /locus_tag="AC06IO_001255"
     CDS             20706..21656
                     /locus_tag="AC06IO_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874456.1"
                     /translation="MMPKRIWLLLLSMFLFLSGSVFAVEDPEEDKVVQTNEPTVGSWV
                     FTGMVTNESGDRYGYFFQMQRQGTDFYAKTALIDGQTNQLILFYEASEKIDSSTQLNW
                     QVGRSFLRYNPINDSWIFGVKVENAKGFNFKVDMLKQDNKDVETQVLRPGVELQAVQT
                     SRLNGHVQTGVDNKEQFVTGNKAWFSKLWFSKDQNSPHDISTIFCRLSNDNGFYSANL
                     KEPDATGAAVAGWRDPSGNNVKMSQFVSIKTLTDNQCLLSVGLPRLKLKLVNTIKQED
                     DFPLTVAGFSEENPKGFCFISKQTFKQIKESAVQAVTNLG"
     gene            complement(21658..>22895)
                     /locus_tag="AC06IO_001256"
     CDS             complement(21658..>22895)
                     /locus_tag="AC06IO_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874457.1"
                     /translation="NIIQTTLNNLSAIKKSKITIWLVGVFFLLFQFFLQLSSGIIVGA
                     IMHEEHISALSAGLLSSAFYYVYTSFQIPVGILFDRYNTRYLLSVNAALCAIGCFVFA
                     SGHNLPTLFLGRLIIGAGSAFAFIGLSHLLRQHFPLKQYAFMIGLSETLGFTVTVLGM
                     IGMGSVISHFGWRYFINIAGIIGFLIASLCWLCIPNSKPIVSFPHKYKQQIIPILKNK
                     LAWFNGLFVCLEFSVITVFAAMWAVPFLQLKLDCTIKTASILTSMVLLGAGLSCPLLG
                     QISVHLNRRKPLIHASCLSTALLLILVLYLPTQNNLLIGILMFSMGLTCGAYMLAYSI
                     SNELAPPEFLSTCTGFTNTLAMLSAPLLQPLVGFFLDVLSNHKSQYSLADYQLALLIV
                     PFAMLLASLFTCLLPEKSN"
BASE COUNT         6823 a         4314 c         4571 g         7187 t
ORIGIN      
        1 taagtaaaaa ttaagctaat ttattaataa ttaccaggct ggtattgcat cttctccaaa
       61 cagttttttt gctttctctt tcacttcttc cgaattcaat gccttcacaa acaattccag
      121 ctgtggcctt ttttcagtgt cacggcgtat cacaatgata ttggcataag gtgaatcttt
      181 gccttcagta aataaagcgt cttttgatgg gcttaatcct gctggcaagg caaacgtcgt
      241 gttaattact gcagcgtcaa catcagggag tactctgggt aattgagcgg cgtctatctc
      301 tttgaatttg aattgtttgg ggttactttc gatatcttga ataccgctat tcgttgtatt
      361 tttaagagta atcaaatgag ctttctccaa taacaagaag gcgcgcatct cattactagg
      421 gtcattaggg actgcaatta ttccattttc aggtaattca ctgagagttt tatatttttt
      481 cgaataaata cccattgggt aaacgaaggt tctgccgatt gcctgcaaat cataaccatg
      541 ggacagtata gcggctttta aataaggtaa atgttggtaa acattagcat ccagactacc
      601 atcttgcagt gcttcattgg gcaaattata atcattaaat tcaacaattt ttatgttgag
      661 cccatactct ttttcagcaa cttgttttgc tgtttcaata agttctgttt cagggccagc
      721 aattgtgccg atgaccaaag tattgggagc tggcttacta ctgcaagcta tcagactaat
      781 gattaaaacc aaacaactta aaattctcat tgaacctccc aattaataat gtgtacaacg
      841 tttggccaag tagtctccaa ccatttgtat cagctggacc aaaataatta atacaatcac
      901 agtagctatc ataatgccag cattaaagcg ttgataacca tagttaatcg ctaatgtacc
      961 taatcctccg gcacccacag ctcctgccat ggctgaataa ttaatcagag tgattgctgt
     1021 tactgtaatg gaatgaatta atcctgattg tgcctcaggt aatatgatat gtaaaataat
     1081 ttggcgtgtg tttgctccca ttgaataccc ggtttctata agaccaaggg gtaacgattg
     1141 gagaacatta tcaactagcc ttgcaaaaaa aggagtcgca ccaactgtta atggaacgat
     1201 tgctgcatta atccctatgc tggtaccaac aattagcctg gtaaatggaa taatagcgac
     1261 aagtaaaata ataaaaggta tggaacggaa aatattaatc agcgcagaca acattttatt
     1321 caatctgatg tttggcttta tccgggatga tgagtacagc aatatgccta aaggcaaccc
     1381 aaggagtact gcaaatagag tactaaaaaa caccatatac agtgtttctc ctgttgcgtt
     1441 aagtaaatca taaaccatct ggtaggacat agcctaaaac ctccacagta ataccagctt
     1501 gttcgcaacg attgataaat gcttcaagca ataatggatt cgctgttaat tcaacaacca
     1561 aaacaccaca agtcactcca tcgaaacgat caatatttgc cagtagaata ttgatgtcca
     1621 tgtttaattc tctgctggtt tggctaataa aaggtaccgt tgcttcctcg ccctgaaaaa
     1681 ataatctcac gagaggtttt tcagtagcat agtccgccag cctgcgagta aggcaggttg
     1741 ggagttcagg gctaatttgt gcgtaaagca ttttgcgagc gagactctct ggcttattga
     1801 aaatattaga taatgcagta gtttcaacaa tcttgccatc gaccatgaca gacagtcttt
     1861 ggcatatacg ttttacgaca tccatttcgt gagtaatcaa gacaatagta attccataca
     1921 attcattgat tttttttagt agtgataata tggcgtcagt agttgcagga tcgagggctg
     1981 aagtggcttc atcacataat agaactttgg gagaacaact tagtgctcgc gcaattgcta
     2041 ctctttgttt ttgcccacca ctaagttgtg aggggaaagc atctttctta tctgttaact
     2101 cgacaacagg aagcaactct tcaattttct gtttgatcga ctcttcgtct attccttgaa
     2161 ttctcatcgg aagggctatg ttatcaaaaa cagtttttga atttagcaaa ttaaaatgtt
     2221 ggaagatcat ggcaatttta tgacgtgcca aggctaaatc ttttctggaa aggctcatca
     2281 gattctgccc atcaataacg acttcacctt cgtcaggttt ttccaatata ttgatgcaac
     2341 gcagtaaggt tgattttcct gccccgcttt tccctataac tccaaaaatt tcaccttcct
     2401 gaataaataa attaatatcg tttaaggcga gtttaccaga gaaagatttt tttagcccac
     2461 ataattcaat cattactata acctgggaat atcgcttgcg gggcataaag tggggattag
     2521 aaagcaaccg ctttagccgt attttcaaaa gatatctgag gcactggaaa acaataattc
     2581 ttaataaaaa gcaataattg cattttacct cgattttaga gttttcatgt tcttaaacag
     2641 aggaataaat cttttgagcg tccgcaagct gtgtttcaaa tcgacgctat gcacaagtat
     2701 acacaaactg gattcataaa caaagaaaaa tcttaattct gttgagtatc tcgatttgtt
     2761 tttcatgatt attggttggc ttttggttta tataaaaaga gataatttgc attaaccaga
     2821 tacgcggtgt cattcggtct gaaaataatt attttggttg aggattgaca agataaattc
     2881 ttattggcaa ccttaagccg gttttaatac aaggaatatt attcatgaaa aagatgtttt
     2941 tagcagtttt taccttgtgc tcattcacag cgcaggcagc agtagaatta aaaacttttc
     3001 aaaatatagt agacgccata gctgaaggaa agcgaatcag ttttgtaatt aacttaaaaa
     3061 aatgtacttc ggaaatgcca ttgaactctg caatagtttc agttactccg aatgcagtta
     3121 tggtagtcgg tgatagccgt gttacagcct cagataggca ttttactctt gatgatccgg
     3181 tggctcgcgg tacacctatg tttgattaca gtaagtttaa tttagattct gagggtgatg
     3241 cttcaattaa aaccactgtg ctgaatgcaa gtagttatga gcgattagga agttatcaaa
     3301 tgaattgtaa attaggggat ggttttaaag tattcgggta aatgagtttt cctcaaatta
     3361 tgaggactat taaatttttt tcattcaaat aatattcaaa taacaagtat ttacatgaaa
     3421 gggcaactca tgcagttgcc ctttttattg gttgatatga gatgaatttt aatgtggatt
     3481 cgtgctgaaa atagctattt tcatgcaatt ttagcttttc aattcgttcc tgtctttaaa
     3541 atattctaat gcttgcgggt ttgacaaaga ctgtaagtta cttactgatt gaccatgaac
     3601 aacttgcctg acggcgagct ccactacttt cccgctaaga gttttaggaa tgtctgggac
     3661 ttgtagtatt ttagctggca catgtcgtgg tgaggcattt tttctaatgg ttgttttaat
     3721 cagattgatt aattcatcgt ccagtttttt cccctcacgc aatttaacga ataaaacaac
     3781 tcgtacatca tcctgccaat cttgcccaat gactatacta tcaacaacat catctacttt
     3841 ttcaacttgt ctgtaaattt cagctgttcc tatacgaact cctcccgggt ttaatatagc
     3901 gtcggaacgt ccatgaataa ttaatccatt gtgggcagtt atttcagcga aatcgccatg
     3961 tgcccacacc cctgggaagc gctcaaaata agcatgttta tatgcttgtc tatccttatc
     4021 attccaaaaa cagactggca tggaagggaa gggtttggtg caaaccaact caccacgttt
     4081 ttgtttgatt gaatggcctt gctcatcata caccgcaact tccataccta atccaatgca
     4141 ttgtaattca cccctataaa ccgggagaat gggatttccc aatgcaaaac aagaaataat
     4201 atccgttcct cctgaaatag agcttaattg cagatctttt ttaatatgtt catagacgaa
     4261 atcataattt ttaggtagaa gaggggagcc ggtagacaag atacagcgca actcggataa
     4321 ttgaaattca ctttttggtt ttaccccggc tttttctata gcagatatga atttggcact
     4381 ggttccaaat actgtgacgt tttcttcatc tatcagtcga aacattctat tatctgtggg
     4441 gtaggttgga gaaccttcat acagagtgat agtagctccc aatgccaaag cagaaacagt
     4501 ccagttccac atcatccaac cacacgttgt ataaaaacat aaattgtctg tggatttaat
     4561 atctgaatga agacctaatt ctttcagatg ctgaattaaa gttcctcctg cgccatggac
     4621 aatacattta ggtttacctg ttgttcctga agaaaacata atataaacag gatgatcgaa
     4681 aggcagagaa ataaaatcac attcttttac cggttttata aaatcatccc aatgcgacgc
     4741 gtacggtagt tgctcaagat taatttcttc attaatattc gggcaaataa ctacatgtct
     4801 tagagaggta atcgcagcag gaagttgtat aaccttttct gagctactgt gttttttacc
     4861 ttgatattga tgaccatcac aaataaagag tactttaggt tcaatttgtc ctatccgatc
     4921 aagcgcagcc tgggtaccaa agtcaggtga gcaggatgac caaattgctc ccaaagaagt
     4981 tgttgccagc atggcaatga ttgtataaga agtattaggc atcactgcgg ctacccggtc
     5041 gccagtggtg acaccaactg ctttaaggcc agcagcacat tgagctactt tttcatataa
     5101 ctcagcatag ctaatgacac ttttgcagtc attttcatca aggcttatta gagcagggtg
     5161 attatccctg cgggttaata atttttgggc gaaattgaag cgtgctcctg aaaaccaacg
     5221 tgcttcaatc atttcagaat agtaattcag aatttggtga ggtggtgtat caaagttcaa
     5281 tttaaagaaa aggcaaagag tttcccagaa tgattcggga tgctttactg accaggtatg
     5341 taagtcctgg taattttcaa aaacttgaca atgttttttt gcggcaaaat ccataaattg
     5401 ccacattctt gtttgttcag ggtttttagg tgtccatact ttatcgttca tgaaaatttc
     5461 tctcatagtt ctgtttttaa tgatgggtat tgcactggaa aacttcatca ataatttatt
     5521 tataagtata ggctagcata aaactgggtg cttctcttta agtttcctat ttttagtagc
     5581 tatgaaagct aatactgatg gaactttcta actacatgga ttggctagca tagcattagc
     5641 tactttggat tgattttttc gtcctaatgc tttacaaatc atatctccgg cagcaacaat
     5701 tttgaatata tctatacctg tatcaatccc caatccatgc atcaaataaa gtacatcttc
     5761 tgttgcaaca ttgccgctgg ctcctcgggc atatggacac cctccaagac ccgcaacaga
     5821 gctgtcaaag cggttaactc catactccag agaggtataa atatttgcta cggcttgtcc
     5881 ataagtatcg tggaaatgca tagctaattg tgtgatggga agtacgggca gtatggcatc
     5941 aagaagcaac tgagtttgct tgggagttcc tacgccaata gtatctccca acgaaatttc
     6001 gtgtacgccg agatcaagaa gcattttagt gacactaaca acctgactcg gttgtatata
     6061 tccttcatag gggcagccta atacgcagga gatatagcct ctcactctaa tttgattagt
     6121 ttttgctaat gctaaaacag gtttaaagcg ttctatactt tccttaatag aacaattaat
     6181 attgcgttga ttgaataatt cacttgcagc agtgaatact gcaatatttt gtactcccat
     6241 ttccagagct ttaagcattc ctcgttcgtt tggaaccagg gcagagtagt tgatggaggg
     6301 agtcttatta atagattgaa atacttcttc attatcagct agctgtggaa ttgcttttgc
     6361 tgaaacaaaa ctggtgactt ctatctcttt cagccctgtc tgactgagta aattgattaa
     6421 ttcaattttt ttatcactat ggagaaaaga tggttcattt tgtaagccat ctctaggacc
     6481 tacttcgatt attgtcacat gctgaggata gtccataaat attctcttaa gtatccgatt
     6541 cgctaagtgc taacagttct gctccctcac tcacttggga acctacagaa taaaaaatat
     6601 ctgataatat accatcgatt ggagcatgaa tggtgtgttc cattttcatt gcctcaagta
     6661 caattaagct ttcaccagct tttacctgtt caccaatatt ttttaaaata gcaacaacag
     6721 tggcaggcat tggcgcggtt aactggcctt tatgggcgga agtttgagca tccagtttac
     6781 tccagtagaa gcgttcgatt gaaagttggc ctttgtcagt atagaagatc aaatggtgat
     6841 ctttgttttc tactctggct ttcactgatt tttgatcaat ttcaataata agctggtcaa
     6901 tgtcatatct ggcatagatg accatttcct tgttttcaat ttttgcttta aattttttat
     6961 tatctatagg tgtaatctga gcttcaatta tcgtggagtt caattgataa cgccatatcc
     7021 aatggctgga aagatgcatt tgccagccaa atgcctcttg caacaaggga tccgtagtgc
     7081 gattaatcat acttaaataa tcacaactta tggccaggat catacctaac tgattatctg
     7141 gtttaggcag atttatgttt tctttctcta aaaaatcagt acttaatttt gcctctttaa
     7201 attttacatg ttggcaaatg gctcgtagaa agggtatatt tgtttttacc ccgccaatgt
     7261 catagtgagc caggctacgc tccaatctat ataatgcttc ttcacgatta tgtccccagg
     7321 caatcagttt ggctatcatg ggatcataat accttgttat ttctgaagac aaagcaacac
     7381 ctgtatcaat gcgtatgcca tctccagagg gttcttttaa gaattgaagt tggccaatag
     7441 agggtataaa accctgataa ggatcttcag cgtaaatacg gcactcaatt gcatggcctt
     7501 gagcttgaat ttgattttga agcaaaggta atgtgtcatt cgctgcaatt tttatttgcc
     7561 aagcgacaag atcaagtcct gtaatcattt ctgtgacagg atgttctact tgaagtcttg
     7621 tattcatttc cataaagtag aatttatctt caccatctac caaaaactct actgttccag
     7681 ctcctctgta gttgatagag cgggctactt cacaggctgc ttctgccagc ctttgccgaa
     7741 gcacgggcga taaattggga gcaggggctt cctcgataat tttttgatgc ctgcgctgaa
     7801 tggaacaatc tctttcaaac aaatgtacaa catttccatg attatctgcc atgatttgta
     7861 cttcaacatg acgaggatta agaactaatc gttcaattat catagtatca tccgcaaagg
     7921 aggccataga ttctcttttg gctcctgcta atgcgtcatg aaattccttt tcatcatgta
     7981 ctgctcgcat tccttttcca ccaccaccat tagcagcttt aatcagtact ggaaatccaa
     8041 tttttttagc ttcgctcaac aacttttctt cagactgttc aacgccatga tagcccggtg
     8101 taagtgggac tttcgttttt tcaagaagtt gtttagctaa ttgtttggat gccattgctt
     8161 ccattgcttt aatggaagga ccgatgaaaa caatgcccgc ttgttcacag gctttggcaa
     8221 aatcaggatt ttcagataaa aatccatagc caggatggat ggcttgtgct ccactttctt
     8281 tagctgcctg aataatatgg tcaatattca gataactttc tttagctggt gcttctccaa
     8341 tataacaggc gctatcggct aatcgcacgt gaaggctatt tctatctgct gctgaataaa
     8401 cggcaatagc ctgaataccc atagaatgtg ctgttttaat gattcgacag gctatttcgc
     8461 ctcggttagc aattaatatt ttattaaaca tgtccagaac ctttattcca gttgggtatt
     8521 tctttattga gaaatgcttt aagcccttct tgcccttcat cagatactct tttatgggca
     8581 atcagagaag cagtgtagtg aaccagttct tcatctattt ttttattagc gacgtattgt
     8641 gccagttgtt ttgagttttt aaccgcttct ggagcattat tactgatttg cgaggcgtat
     8701 ttgagtgtaa actccaacaa cgtatcatca gagacgcagt gctgaactaa attaagagaa
     8761 tatgcacgtg tggcatcaaa aacttcagca ctcatgaata acattttcgc tgctcgctcc
     8821 cctatcgctc taactacata gggactaatt actgctggaa taagacctaa tttcacttca
     8881 gaaaaacaaa aacgtgctga agtagatgca atggctatat cacatgctgc agccaatcca
     8941 gctccaccac caaaagcggc accttgaacc atggcgatag taggttttgg gctttgactg
     9001 agactataca ttaaattacc taataccatg ctgtcttcca ggttttcttc ttcagtaaag
     9061 ttagacatgc tttgcatcca ggtcaaatca gcaccagctg aaaaatgctt tccattcgct
     9121 ttaagtacta tgacgcgaac gttagtatca ttaattgcgg agtcaagtct tattttcatt
     9181 tcagtcagca gttgattatc gaacgcatta tgcttgctaa ttcgattcat agtaagtaaa
     9241 cctactttat cttgaatttc atataataaa tcactcatgc ctattgttcc tttcctttta
     9301 catgcgaaat acaccgaaac gagtatcctc aatcggtgca tttaaggctg cagataatcc
     9361 aagtccgagt attttgcgtg tatcctgtgg ggcgatgact ccatcatccc aaagcctggc
     9421 actggcatag taaggatttc cctgagtttc atactgactt ctcatttgag ttttaaattg
     9481 ttcttcttct tcgagtgacc attcctttcc ttgctttgca tatttctccc tggtaatttg
     9541 cgccagaaca ttcgctgctt gttctccacc cattactgag atgcgtgcat taggccaggc
     9601 ccataggaaa cgaggagcat atgcccggcc acacatggca taattacctg ctccaaaact
     9661 tccaccaaca atgatggtaa atttgggtac attagcattg gcaactgctg tcaccatttt
     9721 ggctccatgt tttgcaatac ctgatgcttc gtatttggag cccaccataa atcctgtaat
     9781 attttgcagg aatacaagtg gtattttacg ttgacagcac agctctatga aatggcttcc
     9841 cttttgcgca ctttcactaa aaagtatgcc attattagca ataatcccta taggatatcc
     9901 atataggcga gcaaaaccgc aaaccagggt tgttccaaaa agtgctttga attcatcaaa
     9961 ctctgaacca tctaccactc gagcaataat ttctcttata tcaaaaggct ttctagggtc
    10021 agtgggaatt attccggtaa gatcttcgct atcataaaga ggaggaactg tatccaccct
    10081 atgaatggaa tctggttttt tacgatttaa attgctaatg gcgactcgcg ctaaatgaag
    10141 cgcgtgagcg tcattttccg cataatgatc agatactcct gagtggcgac agtgaacttc
    10201 ggctcctcct aattcttccg cgctgattat ttctcctgtt gctgctttaa cgagaggagg
    10261 tcctccaagg aatatggttg cctgattctt gaccataata gattcatcag ccatggctgg
    10321 tacataggct cctcccgccg tacaagatcc cataaccacg gctatttgag gaatatttaa
    10381 tgctgacatt tgcgcttgat tataaaaaac acgaccaaaa tgttctttat cggcgaagac
    10441 ctgatcttga agcggtagaa aggcacctcc tgaatccacc agatagatgc atggaagatg
    10501 gtttatcaat gcaatttctt gtgctcttaa gtgttttttt acggtcaaag gataatatgt
    10561 tccgccttta acagtagcat cgttgaccac aatcacacat tcgtttcctg ccactcttcc
    10621 aatgccagtg attattcctg cagcgggtat tgagtcttca tagacttggt aggcagcaag
    10681 ttgagatagt tcaagaaaag gactacctgg atccagcaac tggtgtaagc gttctctcgg
    10741 aagtagtttg ccttgttgtt gatgtttggt acgcgctttt tcatctccac ccagagagat
    10801 ttgatgtatt ttttctctta aatcagtaac caatgcttgc atattcgcct gattattttt
    10861 gaattcctga ctgctggtat tgatttgagt agttaactta gccatttttt atccttaccg
    10921 aatcaaaata tcactcaaga ctatgatggt ccatattatc cagacaatcg caataaaatg
    10981 gggcatgtct tttcctttga gtgcccggta aattagcgct gggattcctg caatgattat
    11041 gggtaataac ataaggacta gaattttcct gataatcagt ccccaacctg tttggctgaa
    11101 tataggcgtt aattttaaat taacgtaagt gaaaaacata tcgatgtaaa ctacgataag
    11161 atgggcatat tttgcaaata cgacaattag aatacttagt agaaggtaaa tgagactatg
    11221 tcttagcatg tacatccttt attattgtta taaccaagtt ggtattgata ggtaaaacat
    11281 tatctggtca caaataaaaa tagcaaccag tttgaatata aaaaactggg taaaaataaa
    11341 gtttttaccc aggtcatctt taaccgttaa attagctcaa ttgccatagc tgtagcttca
    11401 ccaccgccta tacataaaga agctattccg cgttttttac cctggtgttt tagcgcatgc
    11461 attaaagtaa ccagaatgcg agctcccgaa gcaccaatgg gatgccctaa agcgcatgcc
    11521 ccgccatgta tattgacttg ttccgggttc aattcaagct gagtaattgc tgccatcgct
    11581 accactgcaa acgcttcatt aatctcaaaa agatccacat catcttgttt ccatgacgcc
    11641 ttgttcatta ccttacggat tgcatcaaca ggagcggttg tgaaccattg tggagcttgg
    11701 gaatggctgg cgtgtgctat aatttttgcc aagggtttaa tgccacgttt ttctgcgttc
    11761 gaagcactca ttaaaattaa gctggccgca ccatctgaaa tagaacttga attggctgct
    11821 gtgactgtac catctgcttg aaaggctggt tttaactgag ttattttggc aagtttggct
    11881 gcatcaggtc cctcatcgac atcaattgtg acatctcctt tgcgggtagt taaggtgacg
    11941 ggagctattt cttctcgaaa ggcaccattc tctattgctt ttagcgctct gttcattgag
    12001 cgtaaggcat attcgtcttg ttgttctctg ctgaaattaa agtgttttgc agttgtttca
    12061 gcgaaacacc ccattaattg tcctttatca taagcatctt ctaatccatc aaggaacata
    12121 tggtctttca gttcaccatg acccagtcga tatcctgctc ttgctttgga taataaatac
    12181 ggggcattgc tcatactttc cataccgcta gccaaaacta cattagctgt acctgccttg
    12241 attaaatcat gtgcaaacat cacagctttc atgcccgaac cacacatttt attaatggtc
    12301 gtggctcctg ccgaattagg aatgcccgct tttattgctg cctgtctggc aggcgcttga
    12361 ccaataccag cttgtaatac acaaccactg attacttcat cgatttctgc cggagcaata
    12421 cctgattgag acaacaccgc catatgcgct actgccccta actctgggga ggaaagtgca
    12481 gataaattgc ctagcatagc tcccattggt gttctttttg ctgcgactat cacgacatca
    12541 ttatgttcca ttgtctcatt ccttatgctg tttctttaaa aagttcacga ccaattagca
    12601 ttcttcttat ttcagaagtg cctgccccaa tttcatataa cttcgcatcc cttaataagc
    12661 gtcccgtagg atattcatta atataaccat ttcccccaag aatctgtatt gactgtaaag
    12721 ccatttgtgt tgctttttct gcagtatata aaataactcc tgcagcatcc ttacgactaa
    12781 ccctaccgtt gtcacaagct ttggcaacgg catacagata agctctgcaa gcattcaaat
    12841 cagtgtacat atcagcaagt ttgccttgaa taaattgaaa ctcacctatt gcctgattaa
    12901 attgctttcg ttcatggaca tagggtaata caacatccat acaagcttgc ataattccta
    12961 ctggtccggc agcaagaatt gtgcgttcat aatccaagcc gctcattaat accttgaccc
    13021 cttgatttac tgttcccaga acattttcag aaggtacttc acattgatca aataccaatt
    13081 cacatgtgtt tgacccacgc ataccaagct tgtccagttt ttgcgctgtt ttaaacccgg
    13141 gcattccttt ttcgataata aaggcagtaa tccctttgct tccagcctgc ttgtctgttt
    13201 tagcatagac aaccaaaaca tccgcatcag gaccattggt tatccacatt tttgtgccgt
    13261 caagaatata tttgtctcca gcagaacggg cttgtaattg catactgact acatcggacc
    13321 ctgagttgga ctcactcatt gccaaagcac caatatattc accgcttata agtttaggca
    13381 aatatttttg cttttgctca ttgtttccat ttaaaaaaat ttgattgaca cacaaattag
    13441 agtgcgcacc ataacttaaa ccaactgatg cggaggctct ggaaatttct tccattgcta
    13501 tggtatgcgc taggtatccc atattggcac caccatattc ttcacttact gtgatgccaa
    13561 gtaatcccat ttctcccagt tttctccaga gatgattagg aaaagtattt ttctcatcga
    13621 tttgtgcagc taaaggagct atttcagttt gggcaaattg gttgacgctg tctcgtaaca
    13681 tgtcatatgt ttcgcccagt tgaaagtcta agcctgtgta catgagtgac atcctgttat
    13741 gttgataaat acgctagact atacccaaat gaagcgtact tgtcaccttc ctgtttattt
    13801 ccaagacaat ctttttaacc cactgataca tttgataata tacggatgtc ttattgcgaa
    13861 aaattgaaag aatttgaatg gtaaatattc atttgttcaa tcgcaaggtg gcttttctct
    13921 ccaaactttt taaattttta tcttaaaaac acctttaccc agggcagtgg ttaaaatgat
    13981 tctttaccta ggtaaaaaaa tcacctaatc tttagccaaa gtgtaatcca aatggattta
    14041 gaagaggagt atttctaatg agaaaactgg tattatgggg gcatggaatt gaagactatc
    14101 gtgagatgtt tgatttaccc cagggagtag agaatttacg actattagaa tacggttgcg
    14161 gccctagcgc tgttaattac cagcaaactc aagtgaaaaa aaaaggagta gtgagttgtg
    14221 accccttatt cgtattagat aaagatacgc tgcttgccaa agcaaaaatg atttttgcga
    14281 atatggctga agaggttagg cagcatcagg atcaatttga ctttagccgt aatggtagtt
    14341 tggataaatt gcttcaggaa aggcaagaag gaatgaagca gttttttgcg gattatgaac
    14401 aaggtaaggc tgatggaagg tacctggggc ttaccgatta tcatttacca tttgccgatt
    14461 ttacctttga ttttgctctg agttctcatt atttttttgc agatcttgat gagcaaacgg
    14521 ttgattttca cctgattgca attcgtgaat tagcacgggt agccaaagaa gtgagaattt
    14581 ttccattgat tgatagagaa ggtaacactt cacaatttct aggaccggtt ttattaggct
    14641 tgcaacagga gaactatgga gtggaggtta gggaggttgc ttttcatttg cataaatcag
    14701 gtaatgcaat gctaagagtt tgggcgcaac aatgtgttgt ttaattgaga ttctttttta
    14761 aagtaagtga ctggcataca gggaggaaaa aatggcagta tcaaataaga aaaacagtga
    14821 gtttacacct tccttaagat ctcacatgat tgccgcgtta ttaagtatgg gagcagctgc
    14881 aattatctta tggttggcgt cggggatgac attagctgct gttatcgttg actccttcag
    14941 tttgggtttt tatatagggt ttatgctcct tatgagcgcc gctccggctg tgttggcttc
    15001 accagttatt ttacctggtt tactacttgt tgtatcagga ttgtttcttg gagcggttgc
    15061 tgcaacgttt gcgcatcgct taaaaaacaa tcccagtttg ccacaaaaca ataatggaaa
    15121 tggtctgatc aatgaattaa caaaagataa atctgagttg ctgattagtc atgatgattt
    15181 acaatgtcga tccaactctt tggaaggtga aaacacttta tctcaacaga atgaggataa
    15241 tgttcccggg aaaaaatttg gtttcatgaa attttggggg agcttgggtt tgacttcatt
    15301 tgctagttca ggagtgactg ctttattggg ttggcttgct acagattttg tagtttctct
    15361 ctctataatt cctgtgtttt tgctaggaat gactctagcc atcgttttag ttccattgtc
    15421 aattgggtta tccgggattt ttgcgttacc tgcgactact gcgcttatgt ttgcattggg
    15481 aggttttgct acaggagtgt ttgctgctac cttggccgca tatgttatat ctgataatca
    15541 ggcgcaagat catcctgttg agccaataat cagaagctcg atggagaagc aatgcgaaga
    15601 tcaggtttct catcaaaaaa ttctaaatgg attaaaaaaa gataaattga ctgctaatga
    15661 agaagtgatt aattctgatt taaacgaaca aaaatcaaga ggtccaaaaa tatcaaagag
    15721 tgatactggt gttaaggaag aacatattaa tacagcagag gaatacagtt tttgtggttt
    15781 aagattctag ctttattatg attggaccaa cgattaatag agacgcgcct atgattttcc
    15841 cttttagaca gggagaatag cctatcagcg gaataagctc gtttatgcaa cagggctatt
    15901 tgcaatcgta atgagcaaat catttcgcta tttatctggg cagatgcata aaaaattatc
    15961 tgaattaatt ttatttcgct tcctattaat cgtcagtacg aaaatcatca cgaaaaatgt
    16021 tatcataccc caattgcttt ttattaatga ttctccctgc tttaggagta attttatgta
    16081 ttcactgctg cgccctttac ttttcagatt ggatgcagaa aaagcccatt cattaacatt
    16141 atccttatta cattacctcc ctggttttta ttttcggaaa atggccggtc aacctgttca
    16201 tgctatgggt cttgtattcc ctcatcaagt tggactggca gctggtttag ataaaaatgg
    16261 cgaacatttg gatgcattgg ccaaattggg attttcattc attgaattgg ggacagtaac
    16321 acctaaggga caaacaggta atcccaaacc aaggcttttt cgtattgcag aggcaaatgc
    16381 gattatcaac aggatgggtt ttaacaattc cggagttgat gtactggtag aaaatgtaaa
    16441 aagtgcaaat tataaaggaa tattggggat aaatattgga aaaaataaag agactaattt
    16501 aaatcaagct gctgatgact atctttattg cttccgtaaa gtatatgatc atgcttccta
    16561 tgtcactatc aatatttcct ctcccaatac ccctgattta cgccagttac aacaaggaga
    16621 ttattttgct gaattactcg cccagttaca aaaagaacag ataaaattag cagatcaata
    16681 tggacgccat gtgcctttag tggtgaaagt atctccggac gaaacagatg aaacactaaa
    16741 acaaatggca gatatcattt tacagtatgg aatcgagggt attattgcaa cgaataccac
    16801 ctgttctcgg aaaatggtaa aaaatttgcc ttgctctgaa gaacagggcg gattaagtgg
    16861 taggccactg atggaattat ctactcgctg cttgcgcttg ttgaaacaat acgttgggaa
    16921 tgatgtgaca ttgataggag tgggtggtat tgacagtttg gagagtgcca aagataagat
    16981 caatgcagga gcttcgttat tacaagtata cagtggttta gtttataagg gaccggagtt
    17041 gattcacgat attgtttcag gtttaaatgc tgtctgatcc tggtggtgag gaaattgatg
    17101 tatattacta aggaattggc tgttcgcatg gaagggtgta tcaagcagag tcatattgag
    17161 gtgacaaagc ggtactcaca aggaaaaatt ctggaaatca acggcggagc agcttgtttt
    17221 tctggttttg agtcatttct atcccaggta gtaggctggg gatttaaaac agagcctaac
    17281 caatataaag ctgaaataga agctatagaa aatttttata aatcgatagg ccataatcgt
    17341 gtcgatattg agttatgtcc ttttgtggga aatcatttaa ttaatttttt aagccaaaga
    17401 ggttactaca taactgagtt aaataatgtt tcagttcttg atttaaagag ttatcatctg
    17461 gtcgctcact ccgagagtga tgggtttatt ataagagaag tgaagcagtc tgaattggaa
    17521 gagtgggcaa aaagagtagc tattggattt ggttttcttg aagctcagga gcaattttac
    17581 caatatgcca gtgcaaaagg agttttagca tttgcagcat atgatcatgg taatttggtt
    17641 gctggcgcca caatagcaat tcatggtgat gcttgtgatt tgggcgttac cagcacatta
    17701 cctctttatc gagggaaagg ccttcagaaa aaattactgt ttgctcgtct gaatctcgcc
    17761 aagcagcatg gtctggaatt cgccacagta accactcaac caggtacagt ttcagatttg
    17821 aacgttcaaa aggctggatt tcgttgtgcc tataccagaa taaaactaac tatggagtag
    17881 gtggacttca tgatagaaca ttggcgctta gacagaataa gagttacaat tcataacatt
    17941 tgcgtacaag attaacttct aaattaatga aaaataatct ccctattaaa tcccgtctgc
    18001 tatacaaacg cttgttaagc tatgtaaaac ctttttggcc agttttatta ttaggagtat
    18061 tggcgaatat cctttattcg ggcattgacg ctggctttac ctatatgacc aagctttttt
    18121 tggataaaag ctttattact attgatctgg attttgttaa acaaatcccg ctgattgttt
    18181 taatcggtat tactctgaga ggattggtca gttctttagg cagttattgt atgacatggg
    18241 tggctcgctc agtagttaaa gtattaaggc agaccgtatt ttctcatatt attcatttac
    18301 cggcggatta ttatgacgaa gccacatcag gtcagcttct ttcaaaaatt ttgtacgatg
    18361 ttgagcaagt ggcacaagtc agtgcggatg ccctaacaga ttttattcaa aatatatgtc
    18421 tggtcatcgg tttgctgacc gttatgatgg ttatatgttg gcagctgtct ttgatgtttt
    18481 tattaactat cccctttgtg ggaattattg ttaattatac caataaaaga gtaagacgaa
    18541 ttagtcacaa agtccaaaaa accatgggcg aagtcacaga aattgccagt gaggcaattg
    18601 agggttatcg ggtggttcgc atctttggtg gtgaacggta tgaaataaca aaatttaata
    18661 aagcaacaga atattcacgc aaaaacgata tgaaagttgc cataagcaag gcgattaatg
    18721 tttcaggtgt tcaattggtg atcgctatcg ggattgccat gattattatg gctgccattc
    18781 atttatctac tgttattaca atcagtgccg gttccttttt ggcaattatc gcagccatgt
    18841 tgcaattaat aaaacctatg aaaactctga ctactcttaa cgcaaccata caaagaggct
    18901 tggctggtgc agaaagtgta tttaatttgc ttgatttgcc tttggaaaga aacaatggtt
    18961 tgatattaaa agacaagata cgtggtgaaa ttgagtttaa gcatgtatac catgcatacc
    19021 gacagggtca aaatatttta catgatgtca attttgtaat tgaagcagga acatctgttg
    19081 ctctggttgg acattcagga agtggtaaaa cgaccatagc cagtttatta ccccgctttt
    19141 atgaattgag tcaaggtatg attactttag atggtatgcc cattcaacag cttagcctgg
    19201 aaagtttacg caagcagatg tctttggtca gtcaaaatgt gacacttttt aatgatactt
    19261 tggctaacaa catagcctat ggtcgttttg atgcgagccg agagcaaatt attactgcgg
    19321 ctaaactggc ctatgctgat gagtttatta agcaattacc tgatggctat gataccaggg
    19381 tcggagaaaa tggggtgttg ttgtcaggtg gacaaagaca aagaatagcg atagccaggg
    19441 caattttgaa agatgctcca attttaatac ttgatgaggc gacttcagct ctggatagtg
    19501 agtctgaaca ttatattcag gcggctttgg agcaagtgat gaacggtaga actactttaa
    19561 tcattgcgca tcgattgtcg actattaagc atgctcacaa gattatcgta atgcaacatg
    19621 gtcgtatcgt tgagcaaggt agtcatcaag aattacttga tatggatggt cattacgctc
    19681 aactgtataa agtgcagcaa tttggcagga ttaatgagga agtggtagtt taatgtcatt
    19741 ttttgtgaac aggatatggt atggtaacca ttttttacag tggatactgg ttccattttc
    19801 ctggttatac agaattgtta tacgaaccag acgatggtat ttacagcgtt tctgccaaca
    19861 gctgtaccct ataccaatta ttgtagtggg aaatgtgacc gtaggaggag taggcaagac
    19921 gcctctggtc attgaaattg ctaaaaaaat acagcaaaaa ggcttaaaag tagggattgt
    19981 aagtcgtggc tacaaagcag caataaaaca ttttccatac gaagtaaaat taaatgattc
    20041 agctgaatta gtaggcgacg agccgctcat gatggcaaga aaaattaatt gcccagtggt
    20101 tattgcgcct aagcgcaatg aggcagtaag gtacctttta gacaagcatt cggttgaaat
    20161 tattatcagt gatgatggct tgcaacatta taaaatgggg cgatctattg aaattgtagt
    20221 aatagatggg atgcgaaaac tgggtaatgg tttttgtcta cccgctggac cattaaggga
    20281 acctgattca cgattaaaac aagttgattt tgttattgtt aatcaaggtg ctgcagaagg
    20341 tgcttattct atggaattaa ttcctaaaaa catagttcga ttgtcaactc aggaagaagt
    20401 cagtaacgat tcgtttactt ctgaagtagc tgctgtagca ggaattggta acccgcaacg
    20461 attctattct actttaagtc aattaggtat aaaatttaat ccatattctt atcccgatca
    20521 tcatcaattt aaacctcatg atttaaatga tatcgatcta ccagttataa tgacagaaaa
    20581 ggatgctgta aaatgttatt catttagttc agataaatta tattatttac ccgttgaagc
    20641 aaagttgaat gattcctttt gggaagcatt ttggtcacat caacaattac aaggttatta
    20701 ctaatatgat gccaaaacga atttggttat tgctattatc catgttttta tttttaagtg
    20761 gcagtgtttt tgcagtggaa gatccagagg aagacaaagt agtccaaacg aatgaaccaa
    20821 ccgttggaag ttgggtattt acaggtatgg ttaccaatga gagtggtgat cgatacggct
    20881 atttttttca gatgcaaagg caaggtactg atttttatgc taaaaccgca ttaattgacg
    20941 gccaaaccaa ccagttaatt ctgttttatg aggccagcga aaaaatagat agctccactc
    21001 agttgaactg gcaagttggg cgctcatttt tgcgctacaa tccgatcaat gacagttgga
    21061 tttttggagt gaaagtcgaa aatgcaaaag gatttaattt taaagtagat atgttaaaac
    21121 aggataataa agatgttgaa acacaggtat taagacctgg ggtggagttg caagcggtac
    21181 agaccagtcg attaaatggg cacgtgcaaa cgggagtaga taataaagaa cagtttgtaa
    21241 ccggtaataa ggcatggttc agcaaattat ggtttagcaa ggatcaaaat tctcctcatg
    21301 atataagcac tattttttgc agattaagta atgataatgg tttttattct gcaaatctca
    21361 aggaacctga tgcaacgggg gctgcggtag caggttggcg agatccttca ggaaataatg
    21421 tcaaaatgtc tcaatttgtt tctattaaaa ccctaaccga taatcaatgt ttgcttagcg
    21481 tgggtttgcc caggttaaaa ttaaaattag tcaataccat aaaacaagag gatgattttc
    21541 ctctaacagt tgcaggtttt tctgaagaaa atccaaaagg attttgtttt atatccaaac
    21601 aaacttttaa gcaaatcaag gagtcagcag tacaagctgt tactaatttg ggatagtcta
    21661 atttgatttt tctggcagga ggcaagtaaa taagcttgcc aatagcatgg caaaaggaac
    21721 aatcaaaagg gccagttggt agtcagccaa gctgtattgg cttttatggt tactgagcac
    21781 atctaaaaaa aatccaacga gtggttgaag taagggagcc gaaagcattg ccaaggtatt
    21841 ggtgaagcca gtacaagtgg ataagaattc aggtggggcc agttcattag aaatggaata
    21901 ggctaacata taagcaccac aggttaaccc catagaaaac attaaaatac ctatcaatag
    21961 attattttga gttggtaaat ataaaaccag tattaacaat aacgcagttg acagacaaga
    22021 agcatgaatc agcggttttc gtctattgag atgtaccgaa atttgaccca ataaaggaca
    22081 gcttaagcca gcaccgagca agaccattga agttagtata ctggctgttt tgatagtgca
    22141 atccagtttt aattgtaaaa aaggaacagc ccacatagcg gcaaaaacgg taattactga
    22201 gaattccaga caaacaaaaa ggccattaaa ccaggctaat ttatttttta gaatggggat
    22261 aatttgttgt ttatatttgt gtggaaaaga aacgattggc ttgctattgg ggatacacag
    22321 ccagcataaa ctggcgatca agaatccaat gattcctgct atgttaataa aataacgcca
    22381 tccaaaatgg ctgatcacag aacccatgcc aatcatccct aatactgtaa cagtaaatcc
    22441 caacgtttct gataggccta tcataaaagc gtattgtttt aagggaaaat gttggcgtaa
    22501 caaatgactt aaaccaataa aggcaaaggc tgaaccagcg ccaataatta atcgcccaag
    22561 gaaaagagtc gggaggttat gtcccgaggc aaatacaaaa catccgatgg cacaaagagc
    22621 tgcattgacc gataacaaat atcgggtatt gtagcgatca aaaagaatcc cgacaggaat
    22681 ttgaaaagaa gtatagacat agtaaaaggc gctacttaaa agtcctgcgc tgagtgcgga
    22741 aatatgttct tcgtgcatta ttgcgcctac aatgatccca gaggatagtt gcaagaagaa
    22801 ttggaataat aaaaaaaata ctcctaccaa ccaaatggtg atttttgatt tcttaatagc
    22861 tgataaattg ttcaaagtag tttgtattat gttga
//
LOCUS       DBNCWK010000035        22866 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.035, whole genome shotgun sequence.
ACCESSION   DBNCWK010000035 DBNCWK010000000
VERSION     DBNCWK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22866
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.035"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            36..1667
                     /locus_tag="AC06IO_001257"
     CDS             36..1667
                     /locus_tag="AC06IO_001257"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIO9874458.1"
                     /translation="MEHETMEFDVIIVGAGPSGLSAAIKLKQLALASQKEITICILEK
                     GAQVGAHILSGAVLEPRSLKELLPENWQQAPLDTPVNKDLFYFLTQNKSFKLPTPKPM
                     HNEGNFIISLGELCKFLAEQAEALGCEIYPGFAAAEILYNDKNQVIGVATGDVGIDRQ
                     GKKTDNYQPGMHLLAKQTLFAEGCRGQLSQTLMSRYHLRDHSQPQTYGIGIKEIWQVD
                     ASRHEPGKVIHTVGWPLDHATYGGSFLYHLSEQRVAIGFVVGLDYKNPWLNPFGEFQR
                     FKTHPLIKSVLTGGERIGYGARALNEGGWQSLPKLTFPGGALIGDAAGFLNVPKIKGI
                     HTAMQSGMLAAQACFDALSEEKSGQFELSSYSEKINQSWLKQELYAVRNIRPGFKYGL
                     FPGLINAALETYVTRGYSPWTLKNHADHKSLIPAEKAKKINYPKPDGVLTFDKLSSVF
                     LSNTFHEENQPCHLKLKQPKLAIEVNLNQYASPESRYCPAAVYEIIEDVEKGPRLQIN
                     AQNCIHCKTCDIKDPRQNIVWQAPEGGGGPNYSGM"
     gene            1756..3528
                     /locus_tag="AC06IO_001258"
     CDS             1756..3528
                     /locus_tag="AC06IO_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIO9874459.1"
                     /translation="MYQPYLLFAILILSLILFVWNYWRYDFVALFALAISVLTGLVPF
                     NKAFSGFSNPAVITVGCVMILTYAITQSGVLNDFFIKLKKSSKKTSFQVGLYSGISAF
                     LSAFMNNVGALGIMMPITIHAFTEIKKSPSIILMPIAFCSVLGGVITAIGTPPNLLIS
                     NFRNQVLGTPYNMFDFTPVGLPVALMGVLFVSLIGWKLVPVRAKYSKSGDSFQISDYM
                     TEVKVPETSSMCEKTVKEFLEMTKANFELIAVAHEGKKKFAYTDDTIIHCNDILIIEA
                     SPEDLKNILDSNGLILAGNKPLTSKELYDSDIITMEAVVLPDSSIEGQSASMMRFRSR
                     HKINLLAISREELSFRKKLIDIRFAAGDVVLLQGNAATLRETIVDMGFLPLAERDINV
                     HLTQKKFLPIICFTLSIILASLQILPLDFSFMIAVLALILLKAIPTHSLYRTIDWSVL
                     LLLAALIPVGEALQTTGAADLIANGFMKIAGQYSPVFALVAILVITMTLSDLLNNAAT
                     ALIMAPIAIKIAQSAHVNVDSFLMAVAIGASCSFLTPIAHQNNTMVMGPGRYRFSDYF
                     RLGLPLELIVVVVTIPTILWVWPL"
     gene            complement(3631..4086)
                     /locus_tag="AC06IO_001259"
     CDS             complement(3631..4086)
                     /locus_tag="AC06IO_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HIO9874460.1"
                     /translation="MKQIKGILLSIISLSFLPFLVFASDASISLVGVQGYDLVSYHQK
                     NGPVRGSGNHTAYHNGVAYLFATDENKKVFQANPEKYLPAYGGYCAFAVSVGRKVVSD
                     PLAWKIVDGILYLNLNKQVQEFWSKDIPGNIKKGNIQWEKIKDIDPQKI"
     gene            complement(4134..5198)
                     /locus_tag="AC06IO_001260"
     CDS             complement(4134..5198)
                     /locus_tag="AC06IO_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1273 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874461.1"
                     /translation="MKIFKGLKKNTGFRKQSNTQKISNSSIQSSITSLSGDASQPRFD
                     FFPINNRDVLLKSPSFNHIGSEFQPMVSKRDVNAISITLPIKHNVHRNHNSNLPVDST
                     SVVDSSNQQDHISYLHICFTHEEGKWYLFLKCFTTLDVFNYDFAPLLKILHKPDIRTY
                     PMSLGCPGVIDRTELVDMDRTIYVRQFIIHGPDDEAKILQDIHDFNMNCLRRFEELTY
                     GRDNSAYRNSQALGRILHKIITAYQEVTVNEYDIALNRLKIVFQDQVFQNDPQICLSL
                     THIVNALIVLSQKKELNNKGVEIDEHQLHLILKELAKHEGKKFKNNLWFPIGTVDAIR
                     QELEELFISKQTISAAPIMS"
     gene            5539..5615
                     /locus_tag="AC06IO_001261"
     tRNA            5539..5615
                     /locus_tag="AC06IO_001261"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5573..5575,aa:Arg,seq:cct)
     gene            complement(5737..7476)
                     /locus_tag="AC06IO_001262"
     CDS             complement(5737..7476)
                     /locus_tag="AC06IO_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIO9874462.1"
                     /translation="MKTVNSQNGTSAEPSVNLGKLSTAKNLVFRGGGSKGVAYVGALQ
                     SLSEQGVLENIENVAGSSAGAMTAAIVACGGSAELMDYFCSANSLADFVDNRLTKKLL
                     PKSEKSPQVDSSVDYSSISHEQKGSQLGTESSLAEAKLVTKPSKLTFAIASKGAKLVE
                     AMEHVMRMAIFTHFPESLDNELKNVHEKLNAIEDKGSAEYINLAKRQQILEDTQKIIN
                     GLHDPNFDLTFGHLHTLHQYDPQRFKELYVTGTDENGELRIFSHEKDADMPVSIAVRI
                     SASLPGVFDPVIYEGRKYIDGGAANNLPVNIFFNKEEAYTPGVVTTIGVSCENKLDIT
                     EKEFKNPSFMERIMGYLGDFVKKMLCKLDVEGAVKEQKREMLKYIDNGNLFVMVAPPI
                     SITTAQMHIDKEEKEGVIKVGKAFCDMQLKSMIDADYRPQPFDEKTAKILASSGEQQE
                     QSIQKTIDFKSRLQNFKQADRPWSQHVEKTIDRLMLVKGEATQDQIDLCLQWQEIKSV
                     HSNPADRLREFAYAFLASPLAEPVSNQQEINFSKETRKEINLLKNLPGLYETTPDFTE
                     EFNNQRMPSLQRA"
     gene            complement(7671..8906)
                     /gene="flgL"
                     /locus_tag="AC06IO_001263"
     CDS             complement(7671..8906)
                     /gene="flgL"
                     /locus_tag="AC06IO_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HIO9874463.1"
                     /translation="MRISTNQIYQRGLDNLLTQQERLSRLQDQQASGIRVRTPSDDPV
                     ASAQIELIKQRISNIELLQNNRQAADSALRLEEGILSNTVNSLHRLREIQIQSGNPSL
                     SEEDRKTLAVEAQALLNQLLDYANTKDSNGSYMFSGSKSLTQPVSLNLSGQYVYNGDN
                     TQRFQAVTTSLLVAVNDTGDNVFMRIPSGNGRFAIRETVTPNTGTASVSSGSVINEAA
                     FVPDNYTMTFALNSQGNLVVMVSGTLSGNVIPPSGLPDDAPLYQEGSAISFNGMEMVV
                     SGLPKAGDSFSISPAKNESIFSTVQRMINNLNKPYVSSVEKAATQTENNQLLAQIDSA
                     LGCILSVQSDLGARLNQLETAEKANNDYLDISAATLKKLREIDPVAVATELNLQLVNL
                     QAAQLSFTRIQGLSLFNFL"
     gene            complement(8910..10859)
                     /gene="flgK"
                     /locus_tag="AC06IO_001264"
     CDS             complement(8910..10859)
                     /gene="flgK"
                     /locus_tag="AC06IO_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HIO9874464.1"
                     /translation="MSILNIAYSGLNAFQRALDVTGNNIANFKTRGYSRQSIQFTPIA
                     SNRYAGSYIGAGVSVSSIYRNVDQFANAQVRSTLSYRTQYDAFYNQAIQIDKLLSQDG
                     SSISVPLQTFFDSIGQLNSTPDNIATRGVVLKQSQLLAQQFNSLQIKLEEYERNSTLQ
                     VTESVKIINRITKELAEVNGKLLGNNNIPELLDHRDELLKQLSGYTDLSIFDQGDGTI
                     SVGIASGDMLVAGTQQRDLVVGTGKDSIFGTKIFLSSGGNNQVDITDRLTTGMLGGLI
                     DYEKNVLGQASQLIGQMAIGLAQTFNTQHKLGMDMNSQIGKDFFTDFNSPGQMLKRSR
                     ASADNSGTAVLSVNISDISQVKLSDYDLIISDTGANELRLIRKSDGTSTTLTWSSSPP
                     APPAGQVVIDGMTITVNDLSQLANNDHYTLIPTRGAARDFALEIKDAYEIALASPVKT
                     TASLNNTGQGQIILGPVLNTASVNKQFRIDFISDTQYNLVNVTDSTTAGPFAFVPNTN
                     NVIQIPDGITPSYSVVLSGIPKSGDQFTTDYNTGGFGDNRNGLILGNIQQNKIFSNGS
                     ETLFDRYGSLLAEVGGRTNQAKTSFESADILHKQALDFQDSKSGVNLDEEGANLLVFQ
                     QAYQAAGKLMEISNQIMNLLFDIMR"
     gene            complement(10909..11784)
                     /locus_tag="AC06IO_001265"
     CDS             complement(10909..11784)
                     /locus_tag="AC06IO_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HIO9874465.1"
                     /translation="MTIQSIATSDFQGLNELKVQAKNNAKEALPEVAKQFEGIFLQSM
                     LKSMRMGQHFLDESSPFSGKNEATFQEMLDTQYASTIAESKGIGLAALLAKQLENSVG
                     DKANNPVNSSTEVSNTKVTNSEESLSVVDDFVKSVWPTAKQAASLIGLDPKLLVAQAA
                     LETGWGKFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYP
                     SIEHSFHDYISLIKGSERYQMALANAENPEIYVSELNKAGYATDPNYSNKILSIYHGD
                     ELNQAIQRCESSEQS"
     gene            complement(11795..12898)
                     /locus_tag="AC06IO_001266"
     CDS             complement(11795..12898)
                     /locus_tag="AC06IO_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HIO9874466.1"
                     /translation="MRRMLVIRWILAIHLIATQVFAERIKDIATLAGVRVNQLVGYGL
                     VVGLSGTGDKTGTKFTEDSFANMLTQLGINIPPGVRLNSKNIAAVMVTANLSSFMKKG
                     QTMDVNISSIGDSKSLLGGTLLLTPLKGADGRVYAMSQGNVVVSGISASGSDGSSVTV
                     NVPSGGRIPNGATIEADIPNPFYYSNSLTYNLHTPDFTTAKRMSDAINELMGPGTAKA
                     IDAGSVVVTAPKKLSQRVDYVSVLENIEFKPGEAMAKIIINARTGTVVISSNVIVKSA
                     AVSHGNLVVSITETPVISQPNAFASGRTVATQQSQVNIQQKNNRAFILPKGTTLKDIV
                     RGINAVGATPADVISILEALQQAGALSATLIVI"
     gene            complement(12937..13629)
                     /locus_tag="AC06IO_001267"
     CDS             complement(12937..13629)
                     /locus_tag="AC06IO_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HIO9874467.1"
                     /translation="MNRLNIAVSCLATALLFGCEALHPPAPGDNPDYAPTYPVTPDPK
                     ELRKVSGAIYSSETALPLFETPRARHPGDILTVFLIEKTDAQKNATTTQRKNDTTKIT
                     NKLFLGRPISLGSGYSMDFDLDNQRQFNGEGRSIQNNKLAGSISVTVAKVLANGNMVV
                     QGEKWVRINQGNEFVRLSGIVRPQDIKADNTITSDRIANARISYGGTGQINNTNAQGW
                     LSRILWGPLFPT"
     gene            complement(13642..14427)
                     /gene="flgG"
                     /locus_tag="AC06IO_001268"
     CDS             complement(13642..14427)
                     /gene="flgG"
                     /locus_tag="AC06IO_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HIO9874468.1"
                     /translation="MEPALWVSKSGLEAQDKNIATIANNLANVNTTAFKKGRALFEDL
                     IYQNLRQAGAQSSQNSQIPTGINMGTGVHLVATEKIFSQGSLQNTQNALDVAIEGRGF
                     FTVLMPDGTQAYTRDGSFKTDSTGQIVNSNGYPLQPNITIPPQTTKITIGTDGTVSAL
                     VAGNNAPSVIGNIQLTDFINPAGLQPIGQNLYTETVASGTPITDNPGNSGLGTLLQGS
                     LEASNVNVVEELVNMIQAQRSYEITAKSIQTVDSMLQYLTQTL"
     gene            complement(14578..15324)
                     /gene="flgF"
                     /locus_tag="AC06IO_001269"
     CDS             complement(14578..15324)
                     /gene="flgF"
                     /locus_tag="AC06IO_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HIO9874469.1"
                     /translation="MEPILYNSISGSRSDFKRQEIIANNLANINTPGFKADLYQAQTM
                     YMNNANGNNQFSAHSFITQNANGIDTSPGEIITTGRDLDVAIDGDGWMAVQDSQGREA
                     YTRGGSLRLNPNGQLITASGKVVLGDGGPISIPPAQSIEIGSDGTISIVPLEGDVKSL
                     AILDRIKLVTLDKNNIYKNDEGLIQLKSGAATANSNIKLQSGALEGSNVNAIDQMVAM
                     ISAGREFDAQMKLLSTVDDNGQKLAQLLQE"
     gene            complement(15335..16648)
                     /gene="flgE"
                     /locus_tag="AC06IO_001270"
     CDS             complement(15335..16648)
                     /gene="flgE"
                     /locus_tag="AC06IO_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HIO9874470.1"
                     /translation="MVFNTALSGIQASTRDLEVIGNNIANSATIGFKGSRAEFADIYS
                     SSAYGSGGNAVGGGVKLSRVHQSFATGTLSTSNNTLDLAVNGSGFFILSDQGAKIYTR
                     AGQFKLNNENYIVNANNQRLTGLLTDSNGAITGASGDLRINTANITPRASSNVSVGVN
                     MNSQSKAPNVDWVGGASPATDTYNNVTSSTIYDSLGNSHVLSMYFIHADPTALAGSPN
                     AASPAGTENQWYVAFQIDNQNVPPNVGAQNTNNLFRANFNSDGSFAGVFDTSNAALPN
                     NLIPLSFNLNNGSSPLNLNIDLSDSTQFGSPFAVQSAYNNGYTTGSLAGLDIDDSGMI
                     YGRYTNGQSLAMGQIQLANFADPESLQNIGNTSWAETTSSGQPLVSAPGSGGLGLINS
                     GRLEESNVDLTGELVKLISAQRNFQANAQTIRTGDAITQTIINIR"
     gene            complement(16762..17439)
                     /gene="flgD"
                     /locus_tag="AC06IO_001271"
     CDS             complement(16762..17439)
                     /gene="flgD"
                     /locus_tag="AC06IO_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HIO9874471.1"
                     /translation="MTTNSVNGSNSAGGLGLNQIDSDKRQNLGQQDFLRLMVAQVQNQ
                     DPLQPQANGEFLSQLAQFSTNDGITKMQESLQQLASSLQSNQALQASALVGRKVLVNS
                     NTLSLGTEGDVKAAIDMAPGVSNLRAAIYTESGELIKTIPLGQPEPGFFQFSWDGTDQ
                     SNQRLASGRYTIKVTGTYGGQEVALKTMTSANVDSVSLGQNGEDLKLNVAGVGSVYLS
                     QVRQISV"
     gene            complement(17450..17872)
                     /gene="flgC"
                     /locus_tag="AC06IO_001272"
     CDS             complement(17450..17872)
                     /gene="flgC"
                     /locus_tag="AC06IO_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HIO9874472.1"
                     /translation="MSLNAIFDIAASAMTAETVRLTTSAGNMSNANVEAGSPDEVYQA
                     KYPVFKSVQENANQWMGEQIKAGVKLDGIYESDAEPHRRYDPNSPIADKDGFVYTSNI
                     NYVEEMANIISASRSYQMNIELLNTTKQLMQRTLQLGE"
     gene            complement(17875..18267)
                     /gene="flgB"
                     /locus_tag="AC06IO_001273"
     CDS             complement(17875..18267)
                     /gene="flgB"
                     /locus_tag="AC06IO_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HIO9874473.1"
                     /translation="MAINLDSYFGIHAKALIARDQRASVLANNIANVNTPNFKARDVD
                     FNEVLTATMAGNSQQLRVTSDQHINTNADFMTHLKYRMTHHASLDGNTVDKDMETTEF
                     ARNALNYQASLSFLNGKIKSMLTALKGE"
     gene            18483..19418
                     /gene="hemF"
                     /locus_tag="AC06IO_001274"
     CDS             18483..19418
                     /gene="hemF"
                     /locus_tag="AC06IO_001274"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HIO9874474.1"
                     /translation="MPISKTLPYNAIEQIKSYLLQLQNTICVSLESIDGQTRFHEDSW
                     QRAAGGGGKTRIMANGNVFEKAGVNFSHVSGEQLPASASAHREELAGRHFSALGVSLV
                     IHPQNPYVPTTHANVRFFVAEKEDSEPVWWFGGGFDLTPYYGFVEDCEHWHQTALNAC
                     LPFGETIYPKFKRWCDDYFFIKHRNEARGIGGLFFDDYNEISFDHSFELMRSIGDHFI
                     LAYEPIVARRKDIPFGNREKAFQNYRRGRYAEFNLVYDRGTLFGLQSGGRTESILMSL
                     PPIVHWEYNWHPEKGSDEEKLYTDFLPAKDWLKKE"
     gene            complement(19564..20871)
                     /locus_tag="AC06IO_001275"
     CDS             complement(19564..20871)
                     /locus_tag="AC06IO_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874475.1"
                     /translation="MTAGFIGLLKQRKFLPLFLTQFFGAFNDNAYKLAMLTMISYHLS
                     HTQEQSEYYQAIAGALFILPFFIFSATSGQLADKYDKALLTRLVKVLEVLLMIIGGIA
                     LYSGSIFLMMCTLTGMGVHSSFFGPIKYAILPDHLPKKNLLAATGLIEASTFIAILLG
                     TTLGTLTVGGKEAIPYIAIIMTLSAAFSGLVSSLFIPPAPSALHELKVDFNLWRATVK
                     MLKEAKKEYGILISILTISWFWLIGAVMLTKLPDYTHYVLGASAHVFALFLALFSIGI
                     ALGSITINWLLKGQVNLSAVPMAMLAFTFFAFDLYWSSPVGHDLKTPLFNLFNFFTTF
                     THWRIAFDLFMLAFCAGLFVVPLYTYLQIISPVENRARTIAANNIFNALFMVMGTLLV
                     MLLLFFSFSIPQVFLILCFLNLVAAVFLFLGLRTLRRSSEETA"
     gene            complement(21108..21671)
                     /locus_tag="AC06IO_001276"
     CDS             complement(21108..21671)
                     /locus_tag="AC06IO_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIO9874476.1"
                     /translation="MVADLMLCTNFFIFTGGPGSGKTAVLNELNTRGYLTVSEVARSI
                     IQKQQADGGDAIHTGNRKAFRDLMLEQSIADYCKMQSEEKIVFFDRGIPDLYGYSKAF
                     CGEASKEVNEAVRRFRYNKTVFIFPPWPEIYKNDDERQQGYQEALQTYYAVKEGYFDC
                     GYVLLEVPKCPIKDRANFIFQMISKNG"
     gene            complement(21729..22688)
                     /locus_tag="AC06IO_001277"
     CDS             complement(21729..22688)
                     /locus_tag="AC06IO_001277"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HIO9874477.1"
                     /translation="MIDDPIDLLRQYSRKLVRELGMLQLNKLAAKEQPSYWHTLIEIN
                     KEPNITISKLSQLLLVSLPTLSRIVNSLINDELVTVNEGLDKRERFLRVTDKGKEKVN
                     YIDEYSNTKIKRAFHYLTDEEREQVITAIGKYAQALEQSRLIRDQIRILRLSTSRALR
                     KQIVNMIENIQVNEFHLSITPEINASILKAEEEYCYYSSCNFWYAVDEQGGIIGSIGL
                     KKLNHTEGEVKKFFIAPKYRGLGLAQKLMLTLVKNAMKHGFKKLFLGTVAQLNAARRF
                     YEKNGFVLVNKNSLPKEFELCPLDTHFYYCETDKLDKSLRELI"
BASE COUNT         6866 a         4752 c         4005 g         7243 t
ORIGIN      
        1 gatcattttt tcatttgtaa gttagaggag tacgtgtgga acacgaaacg atggaatttg
       61 atgttatcat agtcggcgca gggccttctg gattatccgc tgcaatcaaa ttaaagcaac
      121 tggctttagc ttcacaaaaa gaaattacta tttgtatact ggaaaaagga gctcaggtcg
      181 gcgctcatat tctttctggt gcagttttag aacccagaag cttaaaagaa ttactgccag
      241 agaattggca acaggccccg ctagatactc ccgttaacaa agatctgttt tattttttga
      301 ctcaaaataa atcatttaag ctccctactc caaaacctat gcataatgaa ggaaatttta
      361 ttatcagttt gggagaacta tgtaagtttc ttgcagaaca agcagaagct ctgggctgtg
      421 aaatataccc tggatttgcg gcagcagaaa ttctctataa tgataaaaat caagtcatag
      481 gtgtagctac tggggacgta ggaattgatc ggcaaggaaa aaaaactgat aattatcaac
      541 ctggcatgca tttattagcc aaacaaactt tatttgctga gggatgtcgc gggcaattaa
      601 gtcaaacatt aatgagtcgc tatcatttaa gagatcattc gcaaccacaa acttacggta
      661 tagggattaa agaaatctgg caagttgatg catcccggca tgaacctgga aaagtcatcc
      721 atactgttgg ttggcctctt gatcatgcga cttatggagg ctcctttttg tatcatcttt
      781 cagagcagag agttgccata ggttttgttg taggactaga ctacaagaac ccatggttaa
      841 atccttttgg agagtttcag agatttaaaa ctcaccccct tattaaatct gttctaacag
      901 gcggtgagcg aattggttat ggagccagag cacttaatga ggggggatgg caatctctcc
      961 ctaaacttac ctttcctggt ggcgccttga ttggagatgc tgccggtttc ctaaatgtgc
     1021 ctaaaatcaa aggaattcac accgcaatgc aatctggaat gcttgctgca caagcttgtt
     1081 ttgatgcgct aagtgaggaa aagagtggtc aatttgagct aagctcttac tctgaaaaaa
     1141 tcaatcaatc ctggctaaag caagagctat atgcagtgcg aaatattcgc ccgggattca
     1201 aatacggttt attccccggt ctaattaatg ctgccttgga aacttatgta actcgagggt
     1261 attctccatg gactttaaaa aatcatgcgg atcataaatc gttaattcca gcagaaaaag
     1321 ctaaaaaaat taactatccc aagcccgatg gcgtgctcac atttgataaa ctctcatctg
     1381 tatttttatc aaatacattt catgaagaaa atcaaccctg ccatctcaag ttaaaacaac
     1441 ctaagctggc cattgaagtg aacttaaacc aatacgcttc cccagaaagc cgctattgcc
     1501 cggcagctgt ttatgaaata atcgaagatg ttgaaaaagg gcctcgatta caaattaatg
     1561 cccagaactg tattcattgt aaaacgtgcg atattaaaga tccacgtcaa aatatagttt
     1621 ggcaagcgcc tgaaggtggg ggtggaccga attactcagg aatgtaacca atcaaactca
     1681 aataatgttt ttgggtagag tcctgtaatt attagcaggg atgttgatat aaaacatgaa
     1741 aataaggatt attacatgta ccagccttat cttttatttg ccattttaat tctttctctc
     1801 attttattcg tttggaatta ttggagatac gattttgttg ccctgtttgc actggccata
     1861 tcagtattga ccggccttgt tccctttaac aaggcatttt caggattcag taacccggca
     1921 gtaataacag tcggctgtgt catgatctta acttatgcca tcacccaatc aggcgtatta
     1981 aatgatttct ttataaaatt aaaaaaatct tcaaaaaaaa catcatttca agttggtctt
     2041 tatagtggca tcagtgcatt tctgtctgct tttatgaata atgtaggcgc attagggata
     2101 atgatgccaa tcaccattca tgcttttaca gaaattaaaa aatcgccatc tatcatctta
     2161 atgcctattg ccttttgctc agttttggga ggtgtaatca ctgccatagg tacccctccc
     2221 aacctattaa tttccaactt ccgcaatcaa gtcctgggca ccccatacaa tatgttcgat
     2281 tttactcccg tggggcttcc ggtagcattg atgggtgttc tctttgtttc attgattgga
     2341 tggaaattag ttccagtgcg ggccaagtat agtaaatcgg gagattcatt ccagatttct
     2401 gattacatga ctgaagtaaa agttcctgaa acctcttcta tgtgcgaaaa gactgttaaa
     2461 gagtttctcg agatgactaa agcgaacttt gaactgatag cagttgcgca tgaaggtaaa
     2521 aaaaaatttg catacactga tgatacgatc attcattgca acgatatttt aattattgaa
     2581 gcatctcctg aagatttgaa aaacattttg gattcaaatg gtctaatcct cgctggcaac
     2641 aaaccattaa cctctaaaga gctctacgac agtgatatca tcactatgga agctgttgta
     2701 ttaccggatt ccagtattga aggacaatcc gcttccatga tgagatttcg ttcacgtcat
     2761 aagattaatt tgttagctat ttcaagagaa gaactatcgt ttagaaaaaa attaattgat
     2821 attcgtttcg cagcaggaga tgtggtacta cttcagggaa atgccgcaac acttcgagaa
     2881 acaattgtcg acatggggtt cttgcccctt gcagaaagag atattaatgt ccatttgaca
     2941 caaaaaaaat ttctaccaat catttgtttt acactatcca tcattttggc atctctgcaa
     3001 atactaccac tcgacttttc ttttatgata gcagtgctcg cgctcattct attaaaagca
     3061 ataccaacac acagtttgta tcgtactatt gattggtcgg tactattatt attagctgct
     3121 cttatccctg ttggagaagc tttgcaaacg actggtgccg cagatttgat tgcaaatgga
     3181 tttatgaaaa ttgccgggca atattcccct gtctttgcct tggttgcaat tctggttatc
     3241 actatgactt tatctgattt attaaacaac gcagcaacag cattaattat ggctcccatt
     3301 gcaatcaaga ttgctcagtc agcgcatgtt aatgtggatt ctttcttaat ggcagtggct
     3361 attggtgctt cgtgttcctt cctgactcca attgcccatc agaacaacac catggttatg
     3421 ggtccaggaa gataccggtt ttctgattat ttccgtttgg gattaccatt agaactcatt
     3481 gttgtagtcg ttactattcc aactatttta tgggtatggc cattataaaa atagaaatta
     3541 atatgaatta aacctgtacc ataatcgata aaacacaatg gtacaggtta gagatatttg
     3601 aatagacgca aatggtatat tgcaattaca ttaaattttt tgtggatcaa tatctttaat
     3661 tttttcccat tgaatattac ctttttttat gtttccagga atatcttttg accaaaattc
     3721 ttgtacctgt ttattaagat tgagatataa aatgccatca acaattttcc atgctaatgg
     3781 atcactcact actttccgac ctactgaaac tgcaaaagca caataaccac catatgctgg
     3841 caaatatttt tcaggattag cttgaaacac tttcttgttt tcatcagtag cgaataggta
     3901 agctactcca ttatgataag cagtgtggtt accactacca cggacagggc catttttctg
     3961 atgataagaa accaaatcat agccttgaac accaaccaga ctaatcgacg catcagaagc
     4021 aaacaccaga aacggcaaga atgataaaga tattattgat aatagtattc ctttaatttg
     4081 tttcattttt atcgtcctta attaaattgt aactggtagt atttaaatca ctattaactc
     4141 attataggtg ctgcagatat agtttgcttt gaaataaaca gttcttctaa ctcttgtcta
     4201 attgcatcaa cagtccctat aggaaaccat agattatttt taaatttttt accttcatgt
     4261 tttgcaagtt cttttaaaat gagatgtaat tggtgttcat caatttccac tcctttgtta
     4321 ttaagttcct ttttttgact taaaacaatc aaggcattga ctatgtgggt caaagacaaa
     4381 caaatttgtg gatcattttg aaatacttga tcttgaaaca caatcttaag ccggttcaat
     4441 gctatatcat attcatttac agttacttct tgataagcag ttataatttt atgcaaaatt
     4501 ctccccagag cttgagagtt tctataagca ctattatctc taccataggt taactcttca
     4561 aaccgcctca aacagttcat attgaaatca tgaatatctt gcaagatttt agcctcatca
     4621 tcaggcccat gtataataaa ctgcctgaca taaattgttc tatccatgtc aacgagttct
     4681 gtcctatcaa taaccccagg gcaacccaaa ctcatggggt aagttcttat gtctggttta
     4741 tgtagtattt ttaataatgg tgcaaaatca taattaaaaa catccagcgt tgtgaaacac
     4801 tttaaaaata aataccattt tccttcctcg tgagtaaagc atatatgtaa ataggaaata
     4861 tgatcctgtt gattagatga gtccacgact gatgttgagt caactggcaa attactatta
     4921 tgatttcgat gaacattgtg tttaattggt aatgtaatcg agattgcgtt tacatccctc
     4981 ttggatacca tgggttgaaa ttctgaaccg atatgattga aagaggggct ttttaataaa
     5041 acatctctat tgtttattgg aaaaaaatca aatcggggtt ggcttgcatc accagataaa
     5101 gaagtaatgg atgattgaat cgatgaatta gatatttttt gagtatttga ctgcttccta
     5161 aaaccagtgt ttttctttaa tcctttaaaa attttcatat tttaacatct cctttaatga
     5221 ctcaaatttt gcaatccatt atcaactagc gcaaggtatc ttaattatta atatgcggtc
     5281 aatagatatt gtttcatgtc gtactgttat caggttatct ccatcttgaa agccaatcac
     5341 aaaaccttca ccaagtttaa taaaaattga cttgccgagt tccagaattg agaaatgaca
     5401 gaataattaa gcaggactta tgcccctcaa tccaactagc cctatcaata atctttacat
     5461 tttcatataa gaaatttgct ttcccgttgt cattttccga ctataagggt agacttgcgt
     5521 ttgataaata aagtcaaagt cccggtggca cagatggata gcgcagcccc ctcctaaggg
     5581 gcaggtcgca ggttcgaatc ctgcccggga cgccatttat taatggtcta ttccaaagaa
     5641 cctacaagat tatacaattc aataaatcgg ggctttaaaa atattgtaac ctaaagcatc
     5701 cttgctttaa gtcacactct acaggctatt aactttttac gctctttgta aggatggcat
     5761 tctctgatta ttaaactctt ctgtaaaatc aggagtagtt tcatataatc ctggcagatt
     5821 ttttaataga ttaatttctt ttcttgtttc tttactgaaa ttaatctcct gctgattgct
     5881 gactggctct gccaatggag aagcaaggaa agcataggca aactctctta atctatcagc
     5941 aggattgcta tgtactgatt ttatttcctg ccactgcaga catagatcga tttgatcttg
     6001 agtggcctct cctttgacca acattagtcg atcaatcgtt ttttctacgt gctgagacca
     6061 aggtctgtct gcctgtttaa aattttgcaa tcttgattta aaatcaatgg tcttttgtat
     6121 cgattgctct tgttgctcac cactgcttgc caatattttt gctgtttttt catcaaatgg
     6181 ctgtggccta taatctgcat caatcatgct ttttagttgc atatcacaga acgctttacc
     6241 taccttgata actccctcct tttcctcctt atcaatatgc atttgggcgg ttgttattga
     6301 aattggaggt gccaccataa caaacagatt tccattgtct atatatttca acatttctct
     6361 tttctgttcc ttgacagctc cttcaacatc aagtttacat aacatttttt taacaaaatc
     6421 tcctaaataa cccataatcc tttccataaa ggatggattt ttgaattctt tttctgtgat
     6481 gtccaattta ttctcgcaag aaactccaat ggtagtcact actcccggag tatacgcttc
     6541 ctccttatta aagaagatat tgactggtaa attattagca gcaccaccat caatatattt
     6601 tcttccttca tagattaccg gatcaaaaac tcctggcaat gacgcagaga tacgaactgc
     6661 aatagaaaca ggcatgtcag catctttctc atgagaaaaa atccgcaact caccgttttc
     6721 atctgttcca gtaacataca attctttgaa tcgttgagga tcgtactgat gtaacgtatg
     6781 taaatggcca aaagttaaat caaaattcgg atcatgcagc ccgtttatga ttttttgggt
     6841 gtcttccaga atctgttgtc tttttgcaag attaatatat tcagcgctcc ctttatcctc
     6901 aattgcattg agcttctcgt gaacattttt caattcatta tccagagatt caggaaaatg
     6961 cgtgaaaata gccatcctca tgacatgctc catcgcttca actaatttag cgcctttaga
     7021 tgcaatagcg aaagttaatt tggatggttt cgtaacaagt tttgcttctg ctagactgga
     7081 ttcagtacct agctggctac ccttttgctc atgacttatt gaagaataat ccacagaaga
     7141 gtcaacttgt gggctcttct cgcttttagg aagcaatttt ttagtcaatc ggttatcaac
     7201 aaaatctgct aaactattcg cactacaaaa atagtccatt aactcagccg atccaccaca
     7261 agcgactatc gccgcggtca tagcaccagc agagcttcct gcaacatttt caatgttttc
     7321 caatacccct tgttcagaca aggattgcaa tgctccaaca taagctaccc ctttagagcc
     7381 tcctccgcga aatacaagat tttttgcagt actcaatttt cctagattta cagatggctc
     7441 tgctgatgta ccattttgac tatttaccgt tttcataatt tcacctgcac attgggtatt
     7501 taatacatag taacaagtga atatgaacag catgtgacat gcttatgagt tctttttata
     7561 ggttataact tatagttatg ttgattgatt tacagcaatg gttaagtttg catcagttta
     7621 ttcaacacta ctcagtataa aagatgaaat actctatttt tgatatgctt ttacaaaaag
     7681 ttaaataatg ataatccttg aattcgtgta aagcttaact gagctgcctg gagattgact
     7741 agttgtaaat tcaattccgt tgcaacagca acaggatcaa tttctctgag tttttttaat
     7801 gtcgcagcac tgatatccag ataatcatta ttagcttttt cagcagtttc cagttgattc
     7861 agtcttgcgc ccaaatcaga ctgtacgctc aaaatgcaac ctaaagcact gtcaatctga
     7921 gctaacaatt gattattttc agtctgagta gctgcttttt ctacagacga aacatacggt
     7981 ttatttaagt tatttatcat gcgctgtaca gtagagaaaa tagattcatt ttttgctgga
     8041 ctaattgaaa atgaatcccc tgctttaggt aagccagaca ctaccatttc cataccatta
     8101 aaactaatgg cactaccctc ctgatataat ggtgcatcat ccggtaaacc acttgggggt
     8161 atcacattac cactgagagt accactcacc atgactacca ggttaccctg agaatttaaa
     8221 gcaaaagtca ttgtgtaatt atccggcaca aaagctgctt cattaattac cgaaccggaa
     8281 ctgactgaag ctgttcctgt attaggggtt accgtttctc ttatagcaaa acgcccattt
     8341 ccacttggaa tacgcataaa cacgttgtca ccagtatcat taactgctac cagtaaactc
     8401 gttgtaactg cttgaaaacg ctgtgtgtta tctccgttat aaacatattg ccctgagaga
     8461 ttcaatgaca cgggctgtgt tagtgattta ctcccactga acatataact tccattacta
     8521 tctttagtgt tagcataatc aagtaattga tttaataaag cttgagcttc aacagcgagg
     8581 gtttttctat cctcttcgga aagacttggg tttccagact gaatttgtat ttcacgtaag
     8641 cggtgaagtg aattgactgt attggataat atcccttcct ctaaacgtaa ggcactgtct
     8701 gccgcttgcc gattattctg taatagctca atattgctga tgcgctgttt aatcagctca
     8761 atttgtgccg atgccactgg atcatcagat ggagtccgaa ctcttatgcc gctagcttgt
     8821 tgatcctgga gccttgacaa tctctcttgc tgagtcaaca aattatccaa accccgctga
     8881 tatatctgat tggttgaaat acgcattcat cacctcatga tatcaaacaa caagttcata
     8941 atttgattgg atatttccat caattttccc gctgcctggt aagcttgttg aaagacaagc
     9001 aaattggctc cttcctcatc cagattaact ccacttttgc tatcctggaa atctaaagcc
     9061 tgcttatgga gtatatcggc tgattcaaaa ctggttttag cttgattggt tctgcctccc
     9121 acttcagcaa gcaagctacc atatctgtca aataaggttt cgcttccatt tgaaaaaatt
     9181 ttgttttgtt gtatgttgcc aaggatcaag ccattgcgat tatcaccaaa tccaccagta
     9241 ttgtaatcag tagtaaattg gtctccggat ttgggaattc cggataaaac aactgaataa
     9301 gaaggagtta ttccatcggg aatttgaata acattgtttg tatttggtac aaaggcaaac
     9361 ggaccggctg ttgtactgtc cgttacatta accaggttgt attgagtatc cgaaatgaaa
     9421 tctatcctga attgtttgtt aacagaagcc gtattcaata ctggtcctaa aataatttga
     9481 ccttgcccgg tattatttaa tgacgccgtt gttttcactg gagaagccag cgcaatttca
     9541 taagcatcct taatttccaa ggcaaaatct ctggctgctc ctctggtagg gatgagagta
     9601 taatggtcat tatttgccaa ttgcgataaa tcgtttactg taatcgtcat tccatctata
     9661 actacttgcc ctgctggtgg ggctggtggc gagcttgacc aggttaaggt tgtagaagta
     9721 ccgtctgatt ttcttatcaa tcgcaattca ttagccccag tatcgctaat tatcagatcg
     9781 taatcgctta atttcacttg ggatatatct gagatattga cagataagac agcagttcca
     9841 ctattgtcag ctgaagctct agatcgtttc agcatctgtc ccggggaatt aaaatcagta
     9901 aaaaaatcct tgccaatttg gctattcata tccataccca atttatgttg ggtattaaat
     9961 gtctgagcta aaccaatggc catttgacct attaattggc tggcctgtcc caaaacattt
    10021 ttctcataat ctataagacc gcctaacatg ccggtagtca gcctgtctgt aatatcgact
    10081 tgattatttc ctccgctact taaaaagatc tttgtcccaa aaatagagtc tttacctgta
    10141 cccactacca aatcgcgttg ctgggttcct gccactagca tatctccgct ggcaattcca
    10201 acactaatcg taccatcacc ctgatcaaaa atactgagat cagtatatcc tgacaattgt
    10261 ttgagtaact cgtctctatg gtcaagtaat tccggtatgt tgttattccc taatagtttt
    10321 ccattgacct cagccaactc ttttgttatt cgattaatta tttttactga ctctgttact
    10381 tgcaatgttg aattacgctc atactcttca agttttattt gtagcgaatt aaattgctga
    10441 gctaataact gactctgttt caataccaca ccccttgtcg ctatattatc gggtgtacta
    10501 ttcaattggc ctattgaatc aaaaaaggtt tgtaaaggaa ccgaaatact tgatccatct
    10561 tgtgaaagca atttatctat ctgaattgcc tgattataaa acgcatcata ctgagttcta
    10621 taacttaagg tacttctaac ctgagcattg gcaaattgat caacgtttcg ataaatcgat
    10681 gaaacagata ctcctgctcc tatgtatgaa ccagcatagc gatttgaggc aataggtgta
    10741 aattggatag attgtctgct ataccctctg gttttaaaat tagcaatatt attgccagta
    10801 acatctaggg cacgctgaaa agcatttaat cctgaatagg caatattaag tatagacata
    10861 ttttgttcac tccctgaact aattttttct ttaacttggg cctaactatc aactttgttc
    10921 ggatgattca catcgttgta ttgcctggtt caattcatca ccatgataaa tagacaaaat
    10981 cttattacta taatttgggt cagtagcata ccctgcttta ttaagctcac tgacataaat
    11041 ctcaggattt tctgcattgg ccaaggccat ctggtatcgc tcacttccct taattaatga
    11101 aatatagtca tgaaaactat gctctatgga tggatatttt ctaaaggaag cattgatctt
    11161 aatgggtgta tcggcaatgt attctgttgt tttaacctga attgactcta cttcgctatg
    11221 cgatccggtt tttatattaa ataaattatt actgcttgaa ccatctgcat ctcttgtaac
    11281 aaatttaccc cagccagtct ctaatgccgc ttgcgctacc aacagcttgg gatccaatcc
    11341 aattaatgat gcagcctgtt ttgctgtggg ccacacagat tttacgaaat catcaacaac
    11401 agatagcgat tcttctgaat tagttacttt agtattgcta acctctgtag aagagttaac
    11461 tggattgttt gctttatccc caactgaatt ttccagttgt ttagccagta gagctgccaa
    11521 tcctattcct ttagactcgg caatagtgct agcatattgt gtatccagca tctcttgaaa
    11581 agttgcttca ttcttaccac taaaaggact tgattcatca aggaaatgct gccccatcct
    11641 catgcttttt aacatggatt gcaagaatat tccttcaaac tgtttagcta cttcaggcaa
    11701 cgcttctttt gcattgtttt ttgcctggac ttttaattca tttaatcctt gaaaatcact
    11761 cgtagcaata ctttgtatgg tcatatccat ccctttatat gactattaat gtagcactca
    11821 atgctccggc ctgttgtaaa gcttccagta tggaaatcac atcagcaggt gttgcaccaa
    11881 cagcattgat ccctctgact atatctttca aagtggtacc cttgggcaag ataaaagcac
    11941 gattattttt ttgctgaata ttgacttgtg attgctgcgt agccacggtg cggccagaag
    12001 caaaggcatt aggttgactg ataacgggag tctctgtaat gctaacgacc aaattaccat
    12061 gagatacggc agcagattta acaatcacat tgcttgaaat aaccactgtt ccagtcctgg
    12121 cattgataat aatttttgcc atggcttcgc ctggtttaaa ttcaatattc tcaagtactg
    12181 aaacataatc tactcgttgg gataattttt taggggctgt tactaccaca gaaccagcgt
    12241 ctatagcttt cgctgtgcct ggtcccatta actcattgat ggcatcactc attcgtttcg
    12301 ctgtagtgaa atccggggta tgcaggttgt aagtcagtga gtttgagtaa taaaatggat
    12361 taggaatatc agcttcaatc gtagcgccat tgggaattct tcccccactt gggacgttaa
    12421 cagtgacact tgaaccatca ctcccggaag cactgatacc agatactaca acgtttccct
    12481 gtgacatggc atagacacga ccatccgctc ctttcaaagg agtaagtaat aaagttccac
    12541 ctaataagct tttagagtcg cctatggatg aaatgttgac atccatagtc tgtccttttt
    12601 tcataaagct ggacaaattg gcagtgacca tcactgctgc aatgtttttg gaattgagtc
    12661 taactcctgg aggaatgtta attcctagtt gagtcaacat attagcgaaa ctgtcttcag
    12721 taaatttcgt gccagtttta tcccctgtac cgcttaaacc gactaccaac ccatacccaa
    12781 ccaattggtt aacacgaaca ccagccaaag tagcgatgtc tttgattcgt tcagcaaaaa
    12841 cttgagttgc aatcaaatga attgccaata tccatcgaat aaccagcatt cgccgcatct
    12901 taattccttg tcgtttataa tcttgataat taccaattaa gtcgggaata atggccccca
    12961 taaaatcctt gataaccatc cctgagcatt cgtattatta atttggccag ttccaccgta
    13021 agaaattctc gcattggcaa ttctatcaga ggtaatagtg ttatctgctt ttatatcctg
    13081 gggacgaact atccctgaaa gacgaacgaa ttcattgccc tggttaattc tgacccactt
    13141 ctcgccttga acaaccatat tgccattcgc caacactttg gctaccgtca ctgaaatact
    13201 gccagctaat ttattgtttt gtattgatct gccttctccg ttaaactgcc tttggttatc
    13261 caaatcaaaa tccatgctgt aaccacttcc caaagagatg ggtctaccca aaaagagctt
    13321 atttgtgatt tttgtcgtat cattttttct ctgagtggta gtcgcatttt tctgagcatc
    13381 agttttttcg attaaaaaca ctgttaatat atcacccgga tgccttgccc taggtgtttc
    13441 aaacaaagga agagccgttt cactactata aatagcacca cttacctttc ttaattcctt
    13501 gggatccgga gttacaggat aagttggagc ataatcaggg ttgtcgcctg gtgccggtgg
    13561 gtgtaaggcc tcacacccaa acaacaaggc tgtagctaaa caagagacag ctatatttaa
    13621 tcgattcata acctatctca cttacagagt ttgggttaaa tattgcaaca tgctgtcaac
    13681 ggtctgaatg ctttttgctg ttatttcata acttctttga gcctggatca tattaaccaa
    13741 ttcttcaacc acattcacat tagaagcttc taatgaacct tgcaataaag tacctaatcc
    13801 ggagttgcca gggttatctg taatcggtgt accgcttgct acagtttccg tatataaatt
    13861 ctgcccaatg ggttgcaacc ccgctgggtt tataaaatcg gttaattgga tatttcctat
    13921 aacggatggc gcattatttc ctgcgactaa agcactaacc gtgccatctg ttccaatagt
    13981 aatttttgtt gtttgcggcg gtatcgtaat gttgggctga agaggatacc cattactatt
    14041 aacaatctga cctgtactat cagttttaaa gcttccatca cgtgtatagg cctgtgtgcc
    14101 atctggcatt aagactgtaa aaaagccacg tccttcgata gcgacatcca acgcattttg
    14161 tgtattctga agactgcctt ggctgaatat tttttctgta gcaaccagat gcactcctgt
    14221 ccccatgttt ataccggtag gaatttgcga attttgtgaa gattgagcac cagcctggcg
    14281 aagattttga taaattaaat cctcgaataa agctctaccc tttttaaacg cagttgtgtt
    14341 gacgttagca agattattcg caatagtagc aatattctta tcctgggctt ccaaacctga
    14401 tttactcacc cataatgctg gttccataac ggcctcctgc tattattctt attattatat
    14461 ctgccttaac tatcatttca gattttcaaa tgcatacagc acaccttcca ttcctaaaat
    14521 cacctgtgac acccaatcat tggaaagcgg ctcactctaa ctgaacaata attaaattta
    14581 ttcttgcaat agctgtgcca atttttgtcc attatcatcg actgttgata atagcttcat
    14641 ttgggcatca aattcacgac cagcactgat catagctacc atttgatcaa tcgcatttac
    14701 attactcccc tccaatgcac cgctttgcaa tttaatatta ctattagcgg tagccgcacc
    14761 tgatttcagt tggatcaaac cttcatcatt tttatagata ttatttttat ccagcgtaac
    14821 taatttaatc ctgtctaaga ttgccagaga tttgacatcg ccttctaaag ggacaatcga
    14881 tatagttcca tccgaaccaa tctctatgga ttgagcaggc gggatagaaa ttgggcctcc
    14941 atcacccaaa acaaccttac ccgaggcagt aattaactgt ccattaggat tcaaacgcaa
    15001 actgccccct ctggtataag cttcacgacc ttggctgtcc tgaacagcca tccatccgtc
    15061 cccatcaatg gccacatcca agtctctgcc tgtggtaata atctctccag gagaggtatc
    15121 aattccgttc gcattttgag tgataaatga atgtgcgctg aattggttat ttccattcgc
    15181 attattcatg tacatggttt gtgcctgata taaatcagcc ttaaacccag gggtattaat
    15241 atttgccaaa ttattggcaa ttatctcctg tcttttgaaa tctgaacggc taccagaaat
    15301 ggaattatac aggatcggct ccatgactta cctcctagcg aatattgatg atcgtttgag
    15361 taatcgcatc accggttcga atcgtttgag cattcgcctg aaaattacgc tgagcactaa
    15421 ttaacttgac caattcacca gtcaagtcca cattcgattc ttcaagtctt cctgaattaa
    15481 ttaaccccaa accgccactt ccaggagcgc taaccaatgg ctgtcctgaa gaagtagttt
    15541 cagcccagct ggtattgcct atattttgca aactttctgg atctgcaaaa ttagccagct
    15601 gaatctggcc cattgctaaa gattgaccat tcgtataacg gccatagatc attcctgaat
    15661 catcaatatc cagtccagca aggcttccgg tagtgtagcc attattataa gccgactgaa
    15721 cagcaaaggg actgccaaat tgtgtactat ccgataaatc aatatttaga tttaaaggac
    15781 tggaaccatt gtttaagttg aatgacaaag gaattaaatt attgggcaag gcagcgtttg
    15841 aggtatcaaa tactcccgca aacgagccat cagaattaaa gttagctcgg aataaattat
    15901 ttgtattttg agctcccaca ttaggaggta cgttttgatt atctatctgg aatgctacat
    15961 accattgatt ttctgttcca gcaggtgatg cagcattagg tgaaccagcc aaggcagtag
    16021 gatccgcatg aataaaatac atgctcaata catgtgaatt tcctaaacta tcgtagattg
    16081 ttgacgaggt gacattgtta taagtatcgg ttgccggact agcccctccg acccaatcta
    16141 cattcggcgc tttgctttga gaattcatat taacgcctac tgagacattt gaactggctc
    16201 ttggggtaat attcgcagtg tttattctta aatcgcccga tgctcctgta attgccccat
    16261 tagaatcagt tagtaaacca gtcaaccgtt gattatttgc attgactata tagttctcat
    16321 tattcaactt gaattgcccg gcccgagtat aaatcttagc tccttgatca cttaatataa
    16381 agaagcccga accattgaca gccagatcga gtgtgttgtt tgaagtactt aatgtccctg
    16441 tagcgaatga ctgatgtaca cgcgagagtt taacgccacc acctacagcg ttgccgccag
    16501 agccataagc gctggatgaa taaatatctg caaactcggc tcttgagcct ttaaatccta
    16561 ttgtggcaga gtttgcaata ttattaccta taacttccaa atctctggtt gatgcctgaa
    16621 ttccacttaa tgctgtatta aaaaccataa tagcctcctg ctttataatt ttttagcatt
    16681 tgcccttgac tgctttataa gcaatgcgct tatgacttaa gccatcgttt caataaggcc
    16741 acggtgactg aaatcattga tttatactga tatttgcctt acctgactta aatagactga
    16801 tcctacgccc gctacattaa gctttagatc ctcgccattt tgcccaagac tgacactatc
    16861 gacattggct gacgtcatag ttttcaatgc cacttcttgc ccgccatagg taccagtaac
    16921 ctttattgta tatcttcctg atgctaaacg ttgattactt tggtcagtac catcccaact
    16981 aaattggaaa aaaccgggtt ctggctgccc caagggaata gtttttatca actcgccaga
    17041 ttccgtgtag attgcagcac gaagattaga aacaccaggt gccatatcga tggcagcttt
    17101 tacatcacct tctgtaccca gactcagggt attgctgttt actaacacct tacgaccaac
    17161 caaagcggat gcttgaagcg cttgattaga ttgtagagat gaagctaatt gttgcagtga
    17221 ttcttgcatt tttgtgattc catcattggt gctaaactga gccagttgag ataaaaactc
    17281 gccatttgcc tgtggttgca gaggatcttg attttgaact tgtgccacca tcaaacgcaa
    17341 aaaatcctgt tgtcccaagt tttgtctttt atctgaatca atttgattca aacctaaacc
    17401 acctgctgaa ttggaaccat ttactgaatt ggtagtcatt cccttcccct tattcgccta
    17461 attgcagcgt cctttgcata agttgtttgg ttgtattaag taattcaata ttcatttgat
    17521 aagaacgaga agctgaaatg atatttgcca tttcttcaac gtaattgata tttgacgtat
    17581 agacaaaccc gtctttatca gctattggac tatttggatc gtaccttctg tgcggctctg
    17641 catcactctc ataaatacca tccagtttca cccctgcctt aatttgctct cccatccatt
    17701 gatttgcatt ctcctggact gacttgaaaa caggatattt ggcttgatag acctcatctg
    17761 gactacctgc ttcaacattc gcattactca tattgccagc actggtagtc aatcgcactg
    17821 tttctgcagt cattgcagaa gccgcaatgt caaaaattgc atttaatgac atgattattc
    17881 ccctttcaat gcggtcagca tggacttgat tttgccattt aagaaactca agctggcctg
    17941 ataattcaat gcatttcgag caaactcggt agtttccata tctttatcaa cggtattacc
    18001 atctaaagaa gcatgatgtg tcattcggta tttcaaatga gtcataaaat cggcattggt
    18061 attaatatgc tggtccgaag ttacccttaa ctgctggctg ttaccagcca tagttgctgt
    18121 cagtacttca ttaaaatcaa catcccgggc tttaaaattc ggtgtattaa cattggctat
    18181 gttattagca agaactgatg ccctctgatc ccttgcaatc aacgccttgg catgaatccc
    18241 aaaataagaa tcaagattaa tggccatatt tactcctgtc ttattgttat ttaagtctta
    18301 atcatcattt gacattatgc caattttcca aagcactata tggcttcata cttgtttcca
    18361 aacttcactg aaacataaaa ataataaaaa aatcctattt aaacaaagca aacattgtgc
    18421 caatatctaa aaaatgagaa aatgaagcac ccaagcgcta attccctatg cggagtctca
    18481 tcatgccaat ctcgaagaca cttccttata atgcgataga gcaaattaaa tcttatctgc
    18541 tccaactcca aaatacaatt tgcgtctcac tggagtcaat tgatggccaa accagattcc
    18601 atgaggattc ctggcagcga gctgctggag gcggaggaaa aacaagaata atggcgaatg
    18661 ggaatgtctt tgaaaaagcc ggcgtcaatt tttcacatgt ttcaggggaa caattaccag
    18721 catcagccag tgcacataga gaagaattgg caggaagaca tttcagcgca ttaggggtat
    18781 ctctggtcat tcatcctcaa aacccctatg tgccaacaac acatgccaat gttcgatttt
    18841 ttgtcgctga aaaggaggat tcagagccag tctggtggtt tggtggagga tttgatttaa
    18901 ccccttatta tggatttgtt gaagactgtg agcattggca tcaaacggca ttaaatgcct
    18961 gtttaccttt tggagaaacg atttacccaa aattcaagcg ttggtgcgat gattattttt
    19021 ttattaagca cagaaatgaa gcacgtggga ttggcggctt attttttgat gattacaatg
    19081 aaatcagctt tgatcacagc tttgagctca tgcgtagcat aggcgatcat tttattctgg
    19141 cttatgaacc catagtggct cgccgtaaag acatcccttt tggcaacaga gaaaaagcat
    19201 ttcagaatta ccgcagaggc cgttatgcgg agttcaattt agtctatgat cggggcactt
    19261 tatttggtct tcaatctggc ggaaggactg agtccatatt gatgtccctg cctcccattg
    19321 ttcattggga atacaattgg catcctgaaa aaggcagtga tgaagaaaaa ttatacacgg
    19381 attttttacc tgcaaaagat tggttgaaaa aggagtaaaa caccaaactc aaaaataaat
    19441 gatgtccttc tccccttacc tgaaaatttc gagaagccag tatacatgca gtaaatgaac
    19501 accgcgaaac gtttacaacg cgtaattaaa atggaagagg cagtttatgc cagagatctg
    19561 ttgttaagct gtctcctctg agcttcttct taaggttctt aacccaagaa acaagaacac
    19621 cgcagcaacc aaatttaaaa aacaaagtat caaaaaaacc tgaggaattg aaaagctaaa
    19681 aaacaataat agcatgacta acaaagtacc catcaccata aataaggcat tgaaaatatt
    19741 gttcgccgca atcgttctag ccctgttctc aactggactt ataatttgca agtaagtata
    19801 caagggaact acaaatagtc ctgcacaaaa tgctaacata aataaatcaa aagcaattcg
    19861 ccaatgagta aatgtagtaa aaaaattaaa taagttaaat aagggagtct ttaaatcatg
    19921 acccacagga gatgaccaat acaaatcaaa agcaaaaaaa gtaaaagcga gcatggccat
    19981 tgggacagca gaaagattaa cttgtccttt caataaccaa tttatagtaa ttgatcccag
    20041 agcaatgcct attgaaaata aggcaagaaa taaagcaaaa acatgagcgc tagcccccaa
    20101 aacataatgg gtgtaatccg gtaatttggt cagcatgaca gcaccaatta accaaaacca
    20161 ggaaatagtt aatatagata taagaattcc atattctttt ttcgcctcct ttagcatctt
    20221 cactgtggcc cgccataaat taaaatccac ttttaattca tgaagtgctg atggtgcagg
    20281 aggtataaac aggctggaga ccaaaccaga aaaagcggca cttaaggtca taataatcgc
    20341 gatataaggg atagcctcct tccctccaac agttaacgta cccaatgtag tgcctaataa
    20401 aatagcaata aaagtactgg cttcaattag gcctgtcgct gctaataaat ttttcttagg
    20461 caaatggtct ggtaaaatcg catatttgat aggtccaaag aaagaagaat gcacccccat
    20521 cccagtcaaa gtacacatca tcaagaaaat gctcccactg tataatgcaa tacctccaat
    20581 aatcattaat agcacttcaa gcaccttaac cagacgagtc aacaaagcct tgtcatattt
    20641 atctgccaat tgtccggaag tagcggaaaa gataaagaaa ggtagaatga ataaagcgcc
    20701 tgcaatagcc tgataatatt ccgactgctc ttgtgtatgg cttaaatgat agctaatcat
    20761 agtcaacatc gctagcttat aagcattatc gttaaaagca ccaaaaaatt gagtcaggaa
    20821 aaggggcaaa aactttcttt gtttgagaag accaataaaa cctgctgtca ttaaggctcc
    20881 atttgtgctt taaaaataaa gtaaacaagg atattgatgt attgaacgaa agtaaatgat
    20941 aaataccaga taaaaagctt gtaggatgcc actaccactg tttaatttac aaatgcaaca
    21001 cattgtgatt gttatttcgc gatattaatt ttggacgaat ttatttcagg aattggaatg
    21061 acaaaagaac atacaggaga tgtacattag ccaacacccc aaccggctca tccattttta
    21121 cttatcattt gaaaaataaa attagcccgg tctttaatag ggcatttggg aacctctaat
    21181 aatacatacc cacaatcaaa atacccctct tttacagcat aataagtttg caacgcttct
    21241 tgataaccct gttggcgctc atcgtcattt ttataaattt caggccaggg agggaaaata
    21301 aagactgttt tattataacg aaatcgtcta acagcctcat tcacttcctt actcgcctca
    21361 ccacaaaatg ctttggagta accatacaaa tctgggattc cccgatcgaa aaatacaatt
    21421 ttttcctctg actgcatctt gcaataatct gcaatcgatt gttcaagcat caagtctcga
    21481 aacgcttttc tatttccagt atgtatcgca tcaccaccat cagcctgttg tttttgaata
    21541 atagaacgtg ctacttcaga aactgttaaa tagcctcgag tatttaactc attcaatacg
    21601 gcagttttgc cagagccagg tcctcccgta aatataaaaa aattagtgca tagcattaaa
    21661 tccgctacca taacctgtca tacctttaaa aattgctcat aaattaaatt acaaagcttt
    21721 tagtgtactc atatcaattc tcttaagctt ttatccagct tatctgtttc gcaataataa
    21781 aaatgggtat ccaaagggca cagctcaaat tctttaggta aagaattctt attaacaaga
    21841 acaaatccat tcttttcata aaaacgtctt gctgcgttta actgggctac tgtccccaaa
    21901 aacagctttt taaatccgtg tttcatggcg tttttaacta aagtcagcat taatttttga
    21961 gctaatccta accctcgata cttaggcgca ataaaaaatt tttttacttc cccttcagta
    22021 tgatttaact tctttaaacc gatagaaccg ataatccccc cttgttcgtc aaccgcatac
    22081 caaaaattac aactactata atagcaatac tcctcttcgg cctttaaaat tgatgcattg
    22141 atttcaggag taatcgaaag atgaaactca ttcacttgaa tattttcaat catattaaca
    22201 atttgtttac gcaaagctct ggaagttgac aagcgtaaaa ttctaatctg atcacgaatt
    22261 aaacgacttt gttctaaagc ttgagcatac tttccaattg cagtaattac ttgttccctc
    22321 tcttcgtcag ttaaataatg aaatgctctt tttattttag tattagaata ttcatcaata
    22381 taatttacct tttccttacc cttatcagtg actcttaaaa aacgctctct cttatccagc
    22441 ccttcgttta ccgtaaccaa ttcatcatta atcaaagaat tcactatccg cgacagagta
    22501 ggtaatgaaa ctaacaacaa ttgactcaat ttggaaatag taatattggg ttctttattt
    22561 atttcaatta aagtatgcca ataagaaggc tgctcctttg cagctaactt atttaattgc
    22621 aacataccta attctcgtac caattttctt gaatattgcc tgagtaaatc aattggatca
    22681 tcaatcatct tgccactctc accataaaaa ttatattaat aattgcatat attataaatt
    22741 aatattaaaa taattccata ttttatttat ttttattaaa tatgcttgat ttgaaagtga
    22801 gaaaatccct ttgatgagga taggaaacca ccctttccaa ccctgtgatg ggtacattta
    22861 gagagt
//
LOCUS       DBNCWK010000036        22534 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.036, whole genome shotgun sequence.
ACCESSION   DBNCWK010000036 DBNCWK010000000
VERSION     DBNCWK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22534
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.036"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            128..1738
                     /locus_tag="AC06IO_001278"
     CDS             128..1738
                     /locus_tag="AC06IO_001278"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIO9874478.1"
                     /translation="MNAPLTVLMAQINPTVGAIDANTNKILDVIKNHQASHDVIIFPE
                     LTLSGYPAEDLLFRKEYHDKILINLHDIQDTTKDCYVIVGHPMMHIGDCYNGFSIFYQ
                     GEKIREYHKQKLPNYGVFDEARYFTPGKKDPCVLSIKNHKLGFCVCEDLWQKGPVDDL
                     LDSGISILISLNASPFDYRKYQLREELLRSYAKQGVSIIYVNQIGGQDDLLFDGQSLA
                     MDNQGIIRALAPAFEESLCTVNIDGNKIDGSITPYLDKEALIYKALVCGTRDYVRKNN
                     FPGVLLGLSGGIDSALTLAIAVDALGAEQVHAVLMPSRYTASISNEDALEQLSNLKVA
                     HSIIPIEPMFQSMIESLHPVFKDLPPDTTEENIQARIRGMLLMALSNKTGKMVLTTSN
                     KSETAVGYATLYGDMAGGFAVLKDVLKTQVYELVRYRNKISLVIPERVITRPPSAELK
                     PNQTDQDSLPEYGELDAIIMAYMEQNLSPSEIIAKGFKPQVVNKIIQLIKRNEYKRRQ
                     AAPGIKISNLAFGKDWRYPITNGFNQCI"
     gene            complement(1790..3238)
                     /gene="ravH"
                     /locus_tag="AC06IO_001279"
     CDS             complement(1790..3238)
                     /gene="ravH"
                     /locus_tag="AC06IO_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavH"
                     /protein_id="HIO9874479.1"
                     /translation="MDKEQGAYMPPKDGKELLDIIDNLNEFAEKLLSRIADESDEGCK
                     QLVHVLESARARYNKPIESWLKGWFYQFTRKRGQEIDMAIKGMEAFPDAYTRLQEFKL
                     LVGKGEWEHGSFNYYLFDELIKSVPGYKPLESELVHPIILKLRQKINKRIDEFMSQYL
                     ATQKLIEARKQELLQTHQSAQKFIDNVSITNDLEAAKKSAKENKGVIQFCLIFESDIW
                     KLSWVAATGKVYALEPGEELIQILIDGKIQDIVKDIDNLSALHLKQLKQECVKAREML
                     LGRVQLLINPKDPRTQAELTNDVLKENGTSATFVLRGKPNEYSLWWINTLGFAHEISL
                     ESYASMKTWLNDQSHPLKEEHIPQLKTYLLNVNTVKSIDVKEDMKEFKAQLQECLAAG
                     PKSKTPPASGATKTTNAKKLDLRLFGDVERCLRSQLSERQEEKAAIQEPGKINLNKYE
                     NIATLFGNRAKNMEQHEGQQSKPNDSLSIRSI"
     gene            3404..4042
                     /locus_tag="AC06IO_001280"
     CDS             3404..4042
                     /locus_tag="AC06IO_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIO9874480.1"
                     /translation="MRITLLTTAILATGVASAAVPVDGWYASAFGGYTYIPPNIYNFA
                     YGVFRDEVGYNNGYNAGGRVGYQSNPIRYEVEYTYLRGEARKFNLNFITQLGVTGFTS
                     ANTIMANIYYDCPDMLPAIAPFIGLGIGYASLRTELDSIGPFRPSYFTTSDSAFAYQG
                     TVGITYNFSENYAANLAYRYIATDNSSNFGKTYQAHLASVGAIYRFDQGNYK"
     gene            4052..4789
                     /locus_tag="AC06IO_001281"
     CDS             4052..4789
                     /locus_tag="AC06IO_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIO9874481.1"
                     /translation="MKKLIQLSVLSGLLLSGSTFAFNQPQGFYVGILGEISHGPSGED
                     LIFTEPNRTITANVEYSPVSGGGGAVLGYKISHYRIEGEVLYNRISTGPFTISSCTLQ
                     SPNVLTPTGVCPSAFEVNRLGYDGSSAALYGLLNLYYDFFSSVNESTYVPYIGIGAGQ
                     VRIQNRNNFVKTTPVNLPASAGFSTTMVSGAGQGIVGVGYLMDDFAWLAMDFRFLTTS
                     SLSDFNNSRYTLSTFNVGVNFTFDKST"
     gene            complement(4781..5833)
                     /locus_tag="AC06IO_001282"
     CDS             complement(4781..5833)
                     /locus_tag="AC06IO_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIO9874482.1"
                     /translation="MNENHKELISIGLTVSIVIFSLYIVHKFIPSMIWAAIIVIATYP
                     LYTRWRKLFGNKHNTSAFLFTTLMGLLFLIPLSWLIGILIKESQLFINFLQHINKEGG
                     AAPEFFKNIPLVGDDLIQYWDVNIGKPGSIKEFLSNLHVTLTPTSYYIKQIGVNLAHR
                     SFQVGFTLLTLFFFYRDGDKLLLQIQHIGEYCLGDRWFRYSDRLPSALRGTVNGTIVV
                     GIGVGILMGICYALVGFPAPTLTGFITALAAMIPFVVPIVFIIVALILLSVGSLIGGI
                     IVLVWGTLVMFVADHFVKPVLIGGAIQLPFLAVLFGILGGVETLGLLGLFLGPMVMVL
                     FVTLWQEPQGHKKSSG"
     gene            complement(5833..6315)
                     /locus_tag="AC06IO_001283"
     CDS             complement(5833..6315)
                     /locus_tag="AC06IO_001283"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIO9874483.1"
                     /translation="MNQVNLANRVWQDPVYFIAFGFGSGLMSIAPGTWGTLAAIPLYL
                     LMINAHWSIYLLWTALAFALGVWVSDRVTEDLGVHDYKGIVWDEVVGYLLTMFLAPKG
                     ISWMLIGFILFRIFDIWKPQPIRMIDQRVHGGLGIMLDDVLAAVPAWCIMQILVWSFA
                     "
     gene            complement(6405..7361)
                     /gene="thiL"
                     /locus_tag="AC06IO_001284"
     CDS             complement(6405..7361)
                     /gene="thiL"
                     /locus_tag="AC06IO_001284"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIO9874484.1"
                     /translation="MNEFTLIDHYFKSIPCKRNDVLFGIGDDAACLQVPDGMHLLVST
                     DTLVSGIHFLPQWDPYDIACKAVMVNISDMAAMGAQPCWVTLALTLPELNQSWLDSFA
                     RGIKDSLGLYNVDLIGGDTTKGPLSITLTIMGLTPQGQAIRRCGAQAGDAIIVSGELG
                     AAGLAVKLLDGHKKVNAKDKIELMNKLLHPKPRVDLCHLLRKYATSAIDISDGLGADL
                     NHICVASGVGANLFKEAIPRHPLLNQYCPEQALDLALSGGDDYELCFTVSTQQLDSFN
                     RDLQKENLICFPIGVIEEKPGLRIQSADSPSKELQPRGYSHF"
     gene            complement(7354..7797)
                     /gene="nusB"
                     /locus_tag="AC06IO_001285"
     CDS             complement(7354..7797)
                     /gene="nusB"
                     /locus_tag="AC06IO_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIO9874485.1"
                     /translation="MEKQSIRGKRRARKFALQALYQWLMSGTDLHEIEAQFRTINNMD
                     KVDGEYFCRLLYGIPTHVEALEASLLPYLDREINALNPIELTVLRIGSFELFHCPEIP
                     YKVILDESVSLTKEFGSQEGYRYVNGVLNNLAKQVRSVEVSLDNE"
     gene            complement(7855..8322)
                     /gene="nrdR"
                     /locus_tag="AC06IO_001286"
     CDS             complement(7855..8322)
                     /gene="nrdR"
                     /locus_tag="AC06IO_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIO9874486.1"
                     /translation="MYCPFCHAEETKVVDSRLVADGAQVRRRRECLECHERFTTFETA
                     ELIMPLIIKRDGRREPFHIDNLRSGMLRALEKRPVSVDDLEKAIISITEEIRRRGERE
                     IDSQVVGELVMKELFRLDHVAYVRFASVYKRFKDVSDFRQTIDQMKNEDKEKS"
     gene            complement(8325..9578)
                     /gene="glyA"
                     /locus_tag="AC06IO_001287"
     CDS             complement(8325..9578)
                     /gene="glyA"
                     /locus_tag="AC06IO_001287"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIO9874487.1"
                     /translation="MFDESYSIKNFDDVLFKAISDEKRRQEEHIELIASENYVSPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAEELAISRAKLLFGAHYVNVQPHSGSQAN
                     AAVMMALLSPGDTFMGMALPHGGHLTHGSKVNFSGKLYHSVEYGVDSNTGLIDYDALE
                     KLALQHKPKLIIAGFSAYSRILDWARFREIADKVGAYLMADIAHVAGLVAVGLYPSPV
                     PYADVVTTTTHKTLRGPRGGLILCKENEEIEKKLNSSVFPGMQGGPLMHVIAAKAVAF
                     AEALLPEFKTYQQQVLANARTMCSVLQSRGYDIVSGGTDNHLLLVDLINKGITGKEAD
                     AALGRANITVNKNSVPNDPRSPFVTSGLRLGTPAATTRGFKEREITLLSNWVADVLDN
                     VHDETNISRVKTQVLLLCREFPVYA"
     gene            complement(9606..9917)
                     /locus_tag="AC06IO_001288"
     CDS             complement(9606..9917)
                     /locus_tag="AC06IO_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIO9874488.1"
                     /translation="MRDSLKMLLVGSISMIIIACAASPLSESTGEYLDSSTTTAKVKA
                     SLVDQLGTTGFAVKVKTYKDQVQLSGFVDSQKIKQRAGIIAAGVDGVKSVRNDLIIKT
                     R"
     gene            complement(10042..10479)
                     /locus_tag="AC06IO_001289"
     CDS             complement(10042..10479)
                     /locus_tag="AC06IO_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874489.1"
                     /translation="MKYLIPCVVLFPLLSSCTVIQEDFYQPEYLGPAPRVEVTPSYPQ
                     GHYHRHYNQRYHSNNRYYSAPRGQVHYEHAKTQGHAAVVTPPPSQARVEVQKNVHGHN
                     ANNGQVHGHAHDNGNVHSHPDSSNVHGHDTSNPEEQKKIHGHS"
     gene            complement(10545..10949)
                     /locus_tag="AC06IO_001290"
     CDS             complement(10545..10949)
                     /locus_tag="AC06IO_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874490.1"
                     /translation="MKLKSLLFVVCFSLFSNVFAANLHLHPKADNADKKSIQKSVTYP
                     GYCQIEIINQSFTDVRVFGTFDDGTTIDFNIYRYESPHYISLFYNFYCHSGMYITITS
                     PYYTIYSGWTNVNSTIRVVPYLKEAKAELSTR"
     gene            11190..12452
                     /locus_tag="AC06IO_001291"
     CDS             11190..12452
                     /locus_tag="AC06IO_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9874491.1"
                     /translation="MNKRLTIMGIILLVVFGGIIAFNLIKAYMIKQFFASYAPPAVTV
                     SSAVAQAVDWKPTIDAVGNFVAINGVDVNSEASGNVVQIHFESGQYVEKDEPLITIDD
                     SIDQALLKFNQSELTLKELSYKRQTDLFKRGATPSSSVDEAKANLQQAQAKVEQIQAQ
                     IKQKHIGAPFSGRLGIRQVNLGQYVTPGQTSIVSLQSLDPLYLEFYLPEQYYKRIHLN
                     QPVTFRVEEFPNILFEGVIHAVNSKVDLTTHNVQVQALLPNCPADAIQDPTKSPLIKV
                     RKQVRGDKLIVSCSTELNKQNKIRNFVFIPGMFASIEVDQPAEPNTIIVPSTAISYSL
                     YGNAVYIIEKSKEGKKNKDGTDQLVVNRVFVTTGEQQGNYTVIKKGIKVGQLVVSTGD
                     LKLQNGTPVVINNSVKLDTDSDPNQLGQ"
     gene            12569..15616
                     /locus_tag="AC06IO_001292"
     CDS             12569..15616
                     /locus_tag="AC06IO_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIO9874492.1"
                     /translation="MKFTDLFIKRPVLSIVVSMLIFLFGINSIYKMQIRQYPKMENTV
                     ITIMTGYPGADAELIAGFITTPLESAVASAEGIDYMTSSSQQGLSTITLNIKLNFDPQ
                     IAFTDVMSKVQQTLNQLPKESQQPVILKKSDSSTALMYISLDSKDMTPQQITDYATRV
                     VQPQLQTVDGVAKADILGGQTYSMRIFMDPIKMAALNVTPADVSSVLASNNFLTAAGS
                     TKSEYVAINVTAKTDLNNVGEFERLIVKSDKGSIIRLRDIAKVELGSQNYDSSVTFNS
                     KKAVFISITPTPTANPLTVINDARKLFPSIVREFPPSLTGTIVYDATAFIRASIHEVM
                     ETIIEAAVIVIIVIFLFLGSVRSVLIPVVTIPLSLVGVCTLMLILGYSINLLTLLAFV
                     LAIGLVVDDAIVVVENVHRHLEEGKTPFESAIIGAREIATPVIAMTITLAAVYAPIGF
                     MSGLTGALFKEFAFTLASAVIISGVIALTLSPMMCSKILSEEATSGKFAIFLDEFFNK
                     LKRVYQNALHSILDTREIMLLLAGVVILMLPYLYSNTAAETAPDEDQGFFFVLATAPQ
                     YATLNYIEAFTKPFDAIYKSFPETENYFTVNTSQPISGMVLKPWDQRKKSQFALKQPL
                     QNKLSKIAGLNALAIVPPPLPGGGGGPAVQFVVKTTNDFQSLMDVSNQLTEKARNSGL
                     FIYIDNSLKFNQPQVEFSINRAKASEMGLDMRALGSTLTSALSNNYINYFNLEGRSYQ
                     VIPQLARKFRLTIEQIGQIYVKTLNGTMVPLSTVITPTEKTQPNAATHFQQLNSATIQ
                     AVMMPGKTLGQGLEFLQKAAQEVLPKGFTYDYGGQSRQFMQEGSALIFAFLFAIIVIF
                     LVLAAQYESFRDPLIVLISVPMSICGALIPLNLGAATINIYTQVGLITLIGLISKHGI
                     LIVDFANHLQREKNLDKRAAVEEAAGIRLRPILMTTAAMVFGVIPLLIASGAGAASRF
                     DIGLVISTGLLIGTCFTLFVVPTLYTYLAEDHRHKPNEPAEIQ"
     gene            15872..18637
                     /locus_tag="AC06IO_001293"
     CDS             15872..18637
                     /locus_tag="AC06IO_001293"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIO9874493.1"
                     /translation="MDKTYSPEAIEKALYKKWESHHYFQPRGEGKRFCIMLPPPNVTG
                     SLHMGHGFQHTIMDALTRYHRMLGDKTLWQPGTDHAGISTQLVVERQLEAQGISRKDL
                     TREQFLDKVWQWKEESGNTITQQMRRLGASVDWSRERFTMDEGLSAAVQKVFVQLYEE
                     GLIYRGTRLVNWDPKLGTAVSDLEVLSEEEDGFLWHIRYPVVDSEEFLIVATTRPETL
                     LGDCAVAVHPDDSRFRHLIGKQVHLPLCDRTIPVIADDYVDKEFGSGCVKITPAHDFN
                     DHEVGKRHQLPQINILTKKGTINKNAPLKYQGMDRFVAREQIIKDLEQEGLLAKTEPH
                     KLKVPRGEKSNVIIEPLLTDQWYVKTKPLAEPAIAAVKKGDIRFIPETWDKTYFQWMD
                     NIEDWCISRQLWWGHRIPAWYDNHGNIYVGYSENDVRFKHKIDQSTPLKQDEDVLDTW
                     FSSALWPFSTLGWPERTPELEQFYPTSVLVTGFDIIFFWVARMIMMGLKFTGKIPFKE
                     VFITGLIRDSEGHKMSKSKGNVLDPLDIVDGIDLDSLIAKRTSNLMLNSVRDRITKAT
                     RKEFPEGISAYGTDALRFTYCSLASTGRNVRFDLGRVEGYRNFCNKLWNAARYVLLNT
                     DEEQIDFGDGAFQYSPADQWILSRLQNTVSKVHHYFETYRFDLLANTLYEFVWHEYCD
                     WYLELSKPILQDDQALSAMKRGTRRTLIHVLDQILKLLHPLMPFITEEIWQKTTKFTS
                     ENGISIMLSTYPKVNEEFINPSIEEELDWLKSAIQSLRTIRSEMSISPAKLIPLYIRN
                     ITPELKERIAKYEKILKTLSKIDKINYLAPDEKVPVSATAVLGEIELLIPMADLIDKE
                     AELSRLNKELAKLNKDIELAQGKLNNPKFTDKAPEEIIAKEKDKLAQAQLAKDKLLQH
                     KNRIESL"
     gene            18759..20006
                     /locus_tag="AC06IO_001294"
     CDS             18759..20006
                     /locus_tag="AC06IO_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIO9874494.1"
                     /translation="MVTQFNTLKSYIFPIILLLSILLGGLTGYVFNQNVAWLKPFGDI
                     FLNLIFTTIVPLIFFSVSSAIARTGSIGKLGKIFYSMTVIFLFTGIVAAVYALFVVLL
                     FPPAQNMPVLLESSKQLSPTNLPDRLIEIFTVPEFSKLFSHEHILALVLFSILVGLAV
                     AHSKDKGTIFNSFLKAGEDVFMSVFRLIMYYAPIGFFAYFAVIVSEIGPQLMENYLRI
                     AILYYTASVFYFFFALTAYAYLAGKSHGVRLFWRNIFLPAATSIATCSSAASIPANLM
                     ATKSMHIPAEIYETSIPLGSIIHKDGSVMGGVFKIAFLFGIFHLPFSSLPVLIMALGV
                     SLLVGTVMGAIPSGGMLGELLILSVYGFPPSVLIAVAAISIIIDPIATMVNVTSNSVC
                     SMMIARLVEGKNWFLRSYNENNL"
     gene            20052..20513
                     /locus_tag="AC06IO_001295"
     CDS             20052..20513
                     /locus_tag="AC06IO_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874495.1"
                     /translation="MKKILYVIPGILLITNSFALDIPENITNSNKDLLPSPFPVYVIE
                     GSAVVNHPYPGAKKVLLPTDNSYVDYPGCYIACYSHNTGAYAISPTISVMGQIRVKGQ
                     YDARICQPDGYKNQDISAAYQFKQLCAEKIPACQNNSCWAGGDTGGWFGIQ"
     gene            complement(20614..21627)
                     /locus_tag="AC06IO_001296"
     CDS             complement(20614..21627)
                     /locus_tag="AC06IO_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0716 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874496.1"
                     /translation="MFDDTNMSKKESGTAKPIIKSTLMFFNSKLTDLNDKRLQLNRGK
                     GLAKYLAKSQKNTRDLAPLDAGLGVMSFSLYMLRFSANVGLLAHLILVESQEKTNYKI
                     RRDLYYSLLNDSLWCVVNLTQFFWLSYRNSISAGLQGMQLETLAQLIDVLVMIIRYQQ
                     DKREYDLKYTQATGMERSRLAIEWQNKELNGLRSLLTSLSVVTAFGLFSFSAVAVPLS
                     PVISSIILISSLLRVLIDIEKDRQLIHQLKLNETSREQIKNEQLAMTNARMKDLNKII
                     LNSVFLPIGFFMLITTPIPLTIITCLGMLLVHWLFTNLINMRYSPNVSISPTIQTAEL
                     PLC"
     gene            21760..22431
                     /gene="lciR"
                     /locus_tag="AC06IO_001297"
     CDS             21760..22431
                     /gene="lciR"
                     /locus_tag="AC06IO_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-responsive two-component system response
                     regulator LciR"
                     /protein_id="HIO9874497.1"
                     /translation="MRLLIVEDHKRTADFICKGFNEYQFHADAVYDGAEAIYRISEIN
                     YDAVVLDVMLPIMDGWAVLEKIRELKPDLPVLMLSACDDVDSRVKGLEMGAYDYLVKP
                     FSFNELLARIKSLLRRKPKENPILLKIDDLTLNLQKHTAIRGEKKLPLTAKEFMLLTF
                     MLKRNGEVLSRTVIAEQVWDINFDTNTNIIDVAIKRLREKVDNDHPTKLIHTVRGVGY
                     VLEIR"
     gene            22412..>22534
                     /locus_tag="AC06IO_001298"
     CDS             22412..>22534
                     /locus_tag="AC06IO_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HIO9874498.1"
                     /translation="MYWRFAKSMSITNRLLLIFIGSTVLILSIITGLIYPPMKEL"
BASE COUNT         6986 a         4638 c         4207 g         6703 t
ORIGIN      
        1 taagggagcg gataaatgca aaaataaaaa cttctccaat taacccgact accatcctct
       61 tcatctgtag ccatatcaat gccctctaag ctacaattcc tatatacgaa ttaattgaca
      121 gattttcatg aatgcaccct tgacagtact gatggctcaa attaatccaa cagttggagc
      181 tattgacgcg aatacaaata aaattcttga tgtcatcaaa aaccatcagg caagccatga
      241 tgtgattatt tttccagaat taactctatc cggttatccg gcggaagatt tactctttcg
      301 taaagaatat catgataaaa tactgatcaa cctccatgat attcaggata ctaccaagga
      361 ttgttatgtc attgtcggac atccaatgat gcacattggt gattgctaca atggcttcag
      421 catcttttat caaggagaaa aaatcagaga atatcacaag caaaaattgc caaattatgg
      481 agtttttgat gaagcaagat attttactcc cggtaaaaaa gatccttgtg ttttatcaat
      541 taaaaatcat aaattagggt tttgcgtatg tgaagattta tggcaaaaag gtcctgtaga
      601 cgatttactt gacagtggca tttccatttt aatcagttta aacgcctccc cctttgatta
      661 tcgtaaatac cagctgagag aagagctttt aagatcttat gctaaacagg gggtgtccat
      721 tatttatgtc aatcaaatag gtggtcagga cgatttgctt tttgatgggc aatctttagc
      781 catggataat caaggtatta tccgtgccct ggctcccgcc tttgaagaaa gcttatgtac
      841 tgtcaatata gacggtaaca aaatagacgg atcgatcacc ccttatcttg acaaagaagc
      901 acttatttat aaggcactgg tttgtggtac cagagattac gtcagaaaaa acaatttccc
      961 cggcgtactg ctcgggctct ccggaggcat tgactctgcc ttaacgttag caatagctgt
     1021 cgatgcgctc ggagcagagc aagtccatgc tgttctgatg ccctcacgat ataccgcgtc
     1081 aatcagcaat gaagatgcac tggaacaatt aagcaacctt aaagttgcac attccattat
     1141 accgattgaa cccatgtttc aatctatgat tgaatcgctt catcctgttt ttaaagactt
     1201 gccaccggat accacggaag aaaatattca ggcaagaata cgtgggatgc tgctcatggc
     1261 tttgtcaaat aaaacaggga aaatggttct tactaccagt aacaagagtg aaacagctgt
     1321 aggttatgcc actttatatg gcgacatggc cggcggattt gccgtattaa aagatgtact
     1381 gaaaactcaa gtgtatgaat tagtgaggta tagaaacaag atttcattgg ttatcccaga
     1441 gcgagtcatt accagacccc cttctgctga attaaaacca aaccagacag atcaggatag
     1501 tttacccgaa tacggtgaac tggatgccat tatcatggct tacatggaac agaatttaag
     1561 tccctctgaa atcattgcca aaggatttaa acctcaagta gtcaacaaaa tcattcaatt
     1621 gattaaacgt aatgaataca agagacgtca ggctgcacca ggaataaaga taagtaatct
     1681 ggcctttggt aaagactgga gatatcctat cactaatggt tttaatcaat gcatttgaat
     1741 gcaataactg ccttgaaata aaattcaagg cagttattgc atttattaat taaatagatc
     1801 ttatagagag actatcattt ggttttgatt gttgtccttc gtgttgctcc atgtttttcg
     1861 ctctgttacc gaacaaggtg gcaatattct catatttatt tagattaatt ttaccaggtt
     1921 cttgtattgc agccttttct tcttgccttt cacttaactg gcttctcaaa caccgttcaa
     1981 cgtctccaaa taagcgcaaa tccagttttt tggcattcgt cgtcttggtt gcgccactgg
     2041 caggaggtgt tttagatttt ggtccggcag ccagacattc ctgcaattga gctttaaatt
     2101 ccttcatgtc ttccttgaca tcaatggact tgacagtatt tacattcagt aaataagtct
     2161 taagttgagg aatgtgctct tcctttaacg gatgactttg atcatttaac catgttttca
     2221 tagatgcata ggattctaat gaaatttcat gtgcgaaacc taatgtattt atccaccaca
     2281 atgaatattc attaggttta ccgcgcaaaa caaaagtagc agaagtccca ttttctttta
     2341 atacatcatt agtcaactca gcttgagttc tcggatcttt cggattgatg agcagttgaa
     2401 ctcgtcctaa aagcatttct cgggctttta cacactcctg tttaagctgc ttcaaatgca
     2461 aagcacttag attatcaata tccttaacga tatcctgaat ctttccgtct attagaattt
     2521 ggattaactc ttctccgggt tccaaagcat agactttgcc tgttgcagct acccaggaaa
     2581 gcttccaaat atcactttca aaaattaagc aaaattgtat aacaccctta ttttctttgg
     2641 ctgatttttt agctgcttcc aggtcattgg taattgaaac attatcgata aatttctgag
     2701 cactttgatg tgtctgtaac aattcctgtt ttcgagcttc tattaatttt tgggtagcca
     2761 ggtattgcga cataaactcg tcaattcttt tatttatttt ttgtctgagc ttcaaaataa
     2821 ttggatggac taactcactt tctaatggtt tatagccagg aactgacttg ataagctcat
     2881 caaataaata ataattaaac gatccatgtt cccactcccc tttcccaacc aataacttaa
     2941 actcctgcaa gcgagtgtag gcatcaggga acgcttccat gcctttaata gccatatcaa
     3001 tttcttgtcc gcgttttctg gtaaactggt aaaaccaacc tttaagccat gattcaatgg
     3061 gcttgttata tcttgcccgg gcagattcta agacatgaac taattgctta cacccttcat
     3121 cggattcatc cgcaatccga cttaataact tttcggcaaa ttcgtttaaa ttgtcaatta
     3181 tatccaataa ttcttttcca tcctttggtg gcatatacgc tccttgttcc ttgtccaaat
     3241 ttcatcttac cttaatcaga attttataca agcaacaaaa tcattgagct aactggaatt
     3301 taaaatttaa attgctcaat aaagcgcaat agaaaacttg caaatttaag acaaaccgct
     3361 aacatgaatc agttaaaaaa taataataat aaaaagggat gtcatgagaa ttacattact
     3421 tacaacagct atattagcta ctggtgttgc ctccgccgca gtacctgtag acgggtggta
     3481 tgccagcgca tttggaggtt acacctatat tcctcccaac atatacaatt tcgcttatgg
     3541 ggtattccgt gatgaagtgg gatacaataa cggatataac gccggaggac gagtaggcta
     3601 ccaaagcaac ccaatccgct atgaagtaga atacacctat ctcaggggtg aagccagaaa
     3661 gtttaatctt aactttataa cacagctcgg tgttaccggt tttacctccg ccaatactat
     3721 catggccaat atttactatg attgccctga tatgctgcca gccatagcgc catttattgg
     3781 attggggatt ggttatgcct ctcttaggac agagcttgac agcataggtc cttttagacc
     3841 gtcttacttt accaccagcg acagcgcctt cgcctaccag ggaactgtgg gaataactta
     3901 taatttctcc gaaaattatg cggcaaattt agcttaccgc tatatcgcaa ccgataattc
     3961 gagtaatttt ggaaaaacct accaggctca tttggccagc gtgggagcaa tctaccgttt
     4021 tgatcaagga aattataagt aaggatacgc aatgaagaag ttaattcaac tctcggtatt
     4081 atccggtttg ctattaagcg gttcaacttt tgctttcaac cagcctcaag gcttctacgt
     4141 tgggatattg ggggaaataa gtcatgggcc ttctggtgag gaccttatat ttaccgaacc
     4201 caaccgaacc attactgcca acgtggaata tagtcctgtt tctggaggtg gaggggctgt
     4261 attaggttat aaaataagcc actatcgcat tgaaggtgag gtactatata accggatttc
     4321 aacaggtcct ttcacaataa gttcctgtac tttacaaagt cctaatgtcc taactccaac
     4381 aggcgtttgt ccttccgctt ttgaagttaa tcgactaggt tatgatggca gtagtgcagc
     4441 tctctatggt ttgctcaatc tgtattatga ttttttcagt tctgtgaatg aaagtactta
     4501 tgtaccttat attggaatag gcgcaggaca agtacgtatt caaaatagaa ataatttcgt
     4561 aaaaacaaca cccgtcaatc tgccggcttc agctggattt agcaccacca tggtctcagg
     4621 agctggacag ggcattgtag gcgtagggta tctaatggat gatttcgcct ggcttgctat
     4681 ggacttccgc tttctcacga ccagtagcct gtctgacttt aacaacagtc gctatacact
     4741 cagcacattc aatgtgggtg ttaactttac ctttgacaag tcaacctgat gacttcttgt
     4801 ggccttgggg ttcctgccac aaggtcacaa acaaaaccat caccataggc cctagaaaca
     4861 ggccaagcaa acccaaagtt tccactcctc ccaaaatgcc gaacaaaaca gcaaggaaag
     4921 gtaattgtat cgctccaccg ataagaactg gctttacaaa atgatcagca acaaacataa
     4981 ccaaagttcc ccataccaga acaatgatgc ctccaatcaa actgccaacg gaaagtaaaa
     5041 tcaaagcgac tatgataaaa acaatgggaa caacaaaagg tatcatagct gccaatgcgg
     5101 taataaaccc agtcaatgta ggggctggaa acccaaccaa ggcatagcaa atgcccatta
     5161 atatacctac ccctatgcca acaacaatag ttccattcac agtacctcgc aacgcgctgg
     5221 gaagcctgtc ggaataacgg aaccatctgt cacccaaaca atattctcct atatgctgaa
     5281 tttgtaatag taatttatcc ccatcacgat agaagaaaaa aagtgttaaa agggtaaatc
     5341 ctacttgaaa actacgatga gccaaattga ccccaatctg cttgatgtaa taactggtcg
     5401 gggttaacgt tacatgcaaa ttagataaaa actccttgat acttcccggc ttaccaatat
     5461 tcacatccca atattgaatt aaatcatccc caaccaatgg aatattttta aaaaactcag
     5521 gagctgcccc accttcctta ttaatatgct gcaaaaaatt aataaataat tgagactctt
     5581 tgataaggat acctataagc caactcaagg gaataaggaa aagcaagccc atcagtgttg
     5641 taaataagaa cgctgatgtg ttgtgtttgt taccaaataa tttacgccat ctggtgtata
     5701 agggataggt agctataaca ataatagcgg cccatatcat ggaaggaatg aatttatgaa
     5761 caatgtaaag cgagaagata acgatactga ctgttaaccc tatgctaatt agctctttat
     5821 gattttcatt cattaagcaa agctccaaac caggatttgc ataatacacc aggcaggaac
     5881 tgctgctaaa acgtcgtcca gcataattcc taacccaccg tgaactcttt gatcgatcat
     5941 acgtattggt tggggtttcc agatatcaaa aatacggaac aaaataaagc caattaacat
     6001 ccatgatatt cctttgggag ctaaaaacat agttaataaa taaccaacca cttcatccca
     6061 tacaattcct ttgtaatcat gtaccccaag atcttctgtc actctgtcac tgacccaaac
     6121 tcccagcgca aatgccaggg cagtccataa taaataaatg ctccaatgag cattgatcat
     6181 taataaatag aggggaatag cagctaatgt accccacgtg cctggagcaa ttgacatcaa
     6241 tccactgcca aaaccaaaag cgataaaata aacgggatct tgccatactc gatttgccaa
     6301 attgacttgg ttcatatttg ctcctcaacc taaatatcgc tatcacatca ctttagaatc
     6361 gaattttgtc tttaattttt attatcatac tatagaggcg ggatttaaaa atgtgaatag
     6421 cctctgggtt gcaattcttt acttggacta tcagctgatt gtattcttaa tcctggtttc
     6481 tcctctatca caccgatggg aaaacagatc agattttctt tttgaagatc ccgattaaaa
     6541 gaatccagct gttgtgttga aactgtaaaa cataactcgt aatcatcacc tcctgaaaga
     6601 gccaagtcaa gggcttgttc aggacaatat tgattcaata aaggatgcct gggtattgct
     6661 tctttaaaca agttggcccc aacaccactt gcgacgcaaa tatgatttaa atcagcgcct
     6721 aacccatcag agatatcaat cgctgaagta gcatattttc taagcaaatg gcataaatcc
     6781 acccgtggtt taggatgtaa tagtttattc attaattcta ttttatcttt tgcattcact
     6841 tttttatgcc catccagcaa ttttacagcc agacccgcag cccccaattc accggaaact
     6901 ataatcgcgt cacccgcttg tgcgccacaa cgtctgattg cttgtccctg aggtgtgagc
     6961 cccatgatgg tcaaagtgat tgataacggg cctttggtcg tgtcaccacc gatcagatcc
     7021 acattatata agccaagaga gtcttttatc cctctcgcga aggaatcaag ccaactctga
     7081 ttcaattcgg gcaaggtcaa tgcaagagtc acccaacatg gctgtgcgcc catggcagcc
     7141 atgtcactaa tattgaccat tactgccttg caggcaatat catagggatc ccattgcggt
     7201 agaaaatgaa tgcctgaaac caaagtatcc gtgctgacta gcaaatgcat tccatcggga
     7261 acctgcaagc aagcagcatc atcccctatc ccaaaaagga catcatttct cttgcatggg
     7321 atggatttaa aataatgatc aatcagggta aattcattca ttatcaagac tgacttcaac
     7381 agagcgaacc tgttttgcca aattattcaa taccccatta acgtaacgat atccctcttg
     7441 tgaaccaaac tctttagtca aggatactga ttcgtcaaga atgaccttat aaggtatctc
     7501 agggcaatga aacaattcaa aagatccaat tctcaataca gtaagctcta ttggattcaa
     7561 tgcattgatt tctctatcta aatatggcag taaacttgct tccaaagctt ccacatgagt
     7621 tggaatgccg tataacaggc ggcagaaata ttctccatct actttatcca tattattaat
     7681 tgttctgaat tgagcttcaa tttcatgaag atctgtacca gacattagcc attgataaag
     7741 ggcttgcaat gcaaattttc gagctcgtct tttaccacga attgattgtt tttccacaat
     7801 tacctcatat tattctaata acaacaagaa aagtgagccc ttcacatttt ttatctaaga
     7861 cttttcctta tcttcatttt tcatttgatc aattgtttgt ctaaaatcac tcacatcttt
     7921 aaagcgttta tacactgatg caaaacgcac ataggccaca tgatcaagcc tgaataattc
     7981 cttcattacc aactcgccaa ccacctggga atcgatctcg cgttcacctc ttcttcgaat
     8041 ttcctcagtg atagaaataa tagctttttc caaatcatca acgctgacag gtcttttctc
     8101 caaggctctc aacatgccag aacgtaaatt atcaatatga aacggttctc ttcttccatc
     8161 acgttttata atcaatggca taatcaattc agcggtttca aaagtagtaa accgctcatg
     8221 acattccaga cattcacgcc ttctgcgaac ttgagcacca tccgcgacca gacgtgaatc
     8281 cactaccttt gtttcttccg catgacaaaa tggacaatac atgattaggc ataaaccgga
     8341 aattcacgac agagtagcaa cacctgggtt ttcactcttg aaatattagt ttcatcatga
     8401 acattatcaa ggacatccgc tacccagtta gataacaaag taatctctct ttctttaaag
     8461 cctcttgtgg ttgcagcagg cgtacccaaa cgtaaaccac tcgttacaaa aggcgagcga
     8521 ggatcattgg gaaccgaatt tttattgact gttatgttag ctctgcctaa agcagcgtca
     8581 gcttctttac ctgtaattcc tttatttatc aaatccacta gcaaaagatg attatcagtt
     8641 ccgccagaaa caatatcata gcctctgctt tgtaacacac tgcacatcgt tctggcatta
     8701 gctaaaacct gttgctgata agttttaaat tcgggtaaca gagcttctgc aaaagcaact
     8761 gccttggctg caattacatg cattaagggc ccaccttgca taccaggaaa tacagaagaa
     8821 tttaattttt tctcaatttc ttcgttttcc ttacaaagga ttaaaccacc gcgaggtcct
     8881 cttaaagttt tatgagttgt cgttgtcaca acatctgcat aaggaacagg agaaggatat
     8941 aaccctacag ccaccagacc agctacatga gcgatgtccg ccattaaata agcgccgacc
     9001 ttatcggcaa tttctctaaa tcgagcccaa tccaggatcc tggaataagc tgaaaaaccg
     9061 gcaatgatca attttggttt gtgttgaagc gcaagttttt ccagagcatc ataatcaatc
     9121 aaacccgtat tgctatcaac accatactcg acagaatggt ataatttacc tgaaaaatta
     9181 acctttgacc catgagtcaa gtgcccacca tgaggtaaag ccatgcccat aaaagtatcg
     9241 cctggggaca ataatgccat catgactgct gcgttcgctt gtgatcccga atggggttgc
     9301 acattgacat aatgagcgcc aaaaagtaat tttgcccttg aaatggctaa ctcttcagcg
     9361 acatcaacaa actcacaacc tccgtaatag cgtttgcctg gatatccttc agcgtattta
     9421 ttcgtcaaca cagaaccctg agcctccaat actctcgggc tgacataatt ttcagatgct
     9481 attaattcaa tgtgttcttc ttggcgtcgt ttttcatcag aaattgcttt aaaaagaaca
     9541 tcatcaaaat tttttatact ataactttcg tcaaacatga atgttccttg gttggaaggt
     9601 tatatttacc ttgtttttat aataaggtca ttacgaacac tctttacacc atctacgcca
     9661 gccgcaataa tcccagctct ttgttttatt ttttgagaat cgacaaaacc gctcaactgc
     9721 acttgatctt tatatgtttt tacttttacg gcaaatcccg tagtccccaa ttggtcaacc
     9781 aaactggctt tcaccttggc agtagtcgtt gaactatcaa gatattcccc tgtactttcc
     9841 gacagaggag aagcggcaca cgcaataatt atcatggata tggatcccac taataacatc
     9901 ttaagtgagt cgcgcataga ggctccttaa agataaaaac gttattagag tatcataaaa
     9961 attacccgga tacgaagagg ataatagtac aattttagtt cttttaaaaa aaaagtttcc
    10021 tgtattcagc aagttaaatt attaactatg gccatgaatt tttttctgtt cctcaggatt
    10081 ggaagtatca tgtccatgta cattagaaga atcaggatgg ctgtgcacat taccattatc
    10141 atgagcatgt ccatgcacct gaccgttatt tgcattgtgt ccgtgaacat ttttctgaac
    10201 ttcaactctg gcttgtgatg gtggcggagt aactaccgca gcatggcctt gagttttcgc
    10261 atgctcataa tgaacctgcc cccttggcgc actgtagtat cgattattac tatgatatcg
    10321 ctgattgtaa tgacgatgat aatgcccttg aggataagag ggggtaacct ctacgcgagg
    10381 agcaggcccc aaatactctg gctgataaaa gtcttcttgt ataactgtgc aggaagaaag
    10441 taaaggaaac aatactacgc agggaatgag gtatttcatg tacaactcct caaaggaaag
    10501 gaaaactctc gaatcattga gagctttccc gaattttcaa tgaattatct ggttgataac
    10561 tcagccttag cttctttcaa gtaaggaact acacggatgg tactgttcac atttgtccaa
    10621 ccggaataaa tagtatagta aggtgatgta atagtaatgt acataccact atggcaatag
    10681 aaattataga ataggcttat gtagtgaggt gactcataac gatagatatt aaaatctata
    10741 gttgtgccat catcaaacgt accgaataca cgaacatctg tgaagctttg gttaatgatt
    10801 tcaatttggc agtaaccagg ataagttacg ctcttttgta ttgatttctt gtctgcatta
    10861 tcagccttgg gatgtaagtg aagatttgca gcaaaaacgt tagaaaataa actgaaacat
    10921 acaacaaata acaatgattt taacttcatg agcttcccct tagtaagatg gaagctgcat
    10981 agtatcaaag tattcaattt agtcaatagg atttttttgt acccataaaa tcaaagttca
    11041 ttacaattca attttgttag agaagaagca gaaacaacta tgaaaatatt aaaaataaca
    11101 agtcatctca aacaataaga tgataaagta aatgtttatg ttatatttgc taaattaaaa
    11161 tttttttcaa gcattaacag gggaagataa tgaataaacg attgactatc atgggaataa
    11221 tcttgttggt agttttcggt ggaatcatag cttttaatct aattaaggct tacatgatta
    11281 aacaattctt cgccagttat gcgcctcctg ctgttaccgt ttcttctgct gttgctcaag
    11341 cagtggattg gaagccaacc attgacgctg taggtaattt cgttgcaatc aacggagttg
    11401 atgtcaactc ggaagcatct ggcaatgtag ttcaaattca ttttgaatca gggcaatacg
    11461 tagaaaaaga tgagcccttg atcaccatcg acgacagcat cgatcaagcc ttgctcaaat
    11521 ttaaccagtc ggaattaacg cttaaagaac tcagctataa acggcaaact gatctcttca
    11581 agcgtggcgc cacgccaagc tccagtgttg atgaagccaa agctaacttg caacaggctc
    11641 aggcgaaagt agagcagatt caagcgcaaa ttaaacaaaa acacatagga gccccttttt
    11701 ccggccgcct tggcatccgc caggtaaacc tgggccaata cgtcactcca ggacaaacct
    11761 ccatagtttc tttgcaatca ctggatcccc tttacctgga attttacttg ccagagcaat
    11821 attacaaaag aattcatctt aaccagcctg tgacctttcg cgtagaggaa tttcctaata
    11881 ttctctttga aggagtcatt catgcagtaa actctaaagt agatctaact actcataatg
    11941 ttcaggtaca agccctatta ccgaactgcc ctgctgatgc aattcaggat ccaaccaaat
    12001 ctcctcttat caaagtacgt aaacaagtca gaggagataa actgattgtg tcctgcagta
    12061 cagaattaaa caaacaaaat aaaatacgaa attttgtatt tatcccaggc atgtttgctt
    12121 ccattgaagt ggatcaacct gcagagccta atactattat cgtcccttcc acagccattt
    12181 cttacagcct ctatggaaat gctgtttata tcattgaaaa aagtaaagaa ggtaagaaaa
    12241 ataaagatgg tactgaccag ttggttgtaa accgtgtatt cgtcactacc ggggagcaac
    12301 agggaaatta cacagttatc aagaaaggca ttaaagtagg tcaattggtc gtaagtactg
    12361 gtgacttgaa attacaaaat ggtacccctg ttgttattaa caacagtgtc aaacttgata
    12421 cagacagtga tcccaaccaa ctgggacagt agatcgtgtt ggcaacctgg tatttaaatc
    12481 attttataac attggcatgc tctaataata tggcttgttg ttctttacta ccgactagga
    12541 tcatggtcag ttttttgcag gataacttat gaaattcaca gatctattca tcaagcgccc
    12601 ggtactctcc atagtggtca gtatgttgat ttttctattc gggataaact ccatctataa
    12661 aatgcaaatt cgccaatacc ctaaaatgga gaatacagtc ataactataa tgactggtta
    12721 tcctggcgct gatgctgaac tcatagccgg atttatcact actcctcttg aaagtgctgt
    12781 tgccagtgct gaaggaatag attatatgac ttcatccagt cagcaaggcc ttagcacaat
    12841 taccttaaac atcaagttga atttcgaccc tcaaattgct tttactgatg tcatgagtaa
    12901 agtgcagcaa acgctcaatc agttacctaa agagtcacaa caaccggtga tattgaaaaa
    12961 atcagactca tcaaccgcat tgatgtacat tagtctggac agtaaggata tgactcctca
    13021 gcaaattact gactacgcta cccgagtcgt tcaaccccag ttgcaaacag tggatggagt
    13081 agccaaggct gatattttag gtggtcaaac ttattccatg cgaatattca tggatccaat
    13141 taaaatggct gcgcttaacg tcactcctgc tgatgtgtcc tctgttttag ccagtaacaa
    13201 tttcttgact gcggctggaa gtactaaaag tgaatacgta gccatcaatg tcacggctaa
    13261 aactgatttg aacaatgtcg gcgaatttga aagactgatt gtaaaaagcg ataaggggtc
    13321 aataatcagg ctcagggata tcgctaaagt ggaactgggt tcacaaaatt atgatagctc
    13381 agtgactttc aacagtaaaa aggccgtctt tatatcgatc actccaacac caacggctaa
    13441 ccctctcaca gtaatcaatg atgctagaaa attgtttcct tcgatagtca gagaattccc
    13501 gccgtcatta actggcacaa tagtatacga tgcaacagca ttcattcggg catctatcca
    13561 tgaggtaatg gaaactatca ttgaagccgc agtcatcgtg atcatcgtta tctttttatt
    13621 tttaggctct gttcgttcag ttcttattcc cgttgtcacc attccgctgt cattggttgg
    13681 tgtctgtact ttgatgctca tactcggtta cagcatcaac ttgcttaccc ttcttgcctt
    13741 tgtgttagca ataggattgg tagtggatga tgctattgta gtggtagaaa acgtacatcg
    13801 ccatttggaa gaaggaaaaa ccccctttga atccgcaatt ataggcgcca gagaaattgc
    13861 taccccggtt attgcgatga caatcaccct ggcagctgtt tatgcaccaa tagggtttat
    13921 gagtggactg actggcgctt tgttcaagga atttgctttt acgctggcaa gtgcggttat
    13981 tatttcaggg gttattgcct taacgctgtc ccccatgatg tgttcaaaaa ttctatccga
    14041 agaagccact agtggaaaat ttgctatttt tctggatgag ttttttaaca aactgaaacg
    14101 cgtttaccag aatgcacttc atagcatatt ggatactcga gaaataatgt tgctattagc
    14161 cggggtcgtg attttaatgt tgccctattt gtattcgaac acagccgctg aaaccgcccc
    14221 ggatgaagat caaggcttct tttttgtact ggctactgca ccacaatacg ctaccctaaa
    14281 ctatatcgaa gcctttacaa aaccttttga tgccatttat aaaagttttc ctgaaaccga
    14341 aaactatttt actgtaaaca cgagccaacc tatttcagga atggttttga aaccatggga
    14401 tcaaaggaaa aaaagtcagt ttgctctaaa acaaccttta caaaacaagt tatctaaaat
    14461 tgcaggactt aatgctttag ccattgttcc acctcccctg cctggcggtg gtggtggacc
    14521 tgccgttcag tttgtggtta agacaacgaa tgatttccag agtttaatgg atgtgtcgaa
    14581 ccagctaact gaaaaagccc ggaacagtgg gctatttatt tacatagaca attctcttaa
    14641 atttaaccaa ccacaagtgg aatttagcat caatcgagca aaagcttctg aaatgggctt
    14701 ggatatgaga gcgcttggaa gcaccctgac aagtgctcta tctaacaact acatcaatta
    14761 ttttaatctg gaaggccgaa gctaccaagt catcccacaa ctggctcgca aattccgtct
    14821 tactattgaa cagataggcc aaatttatgt aaaaacactt aacggaacga tggttccatt
    14881 atctactgtg ataaccccaa ccgaaaaaac gcaacctaat gcagcgactc atttccaaca
    14941 actcaattca gcaacaattc aggcggtcat gatgccaggc aagacattgg gtcaaggatt
    15001 ggagttttta caaaaagcag cccaggaagt cttgcccaaa ggatttacat atgattatgg
    15061 aggacagtcc aggcaattta tgcaagaagg aagtgcgctg atatttgcat tcctttttgc
    15121 tattattgtt atctttttag tgctggcagc gcagtatgaa agttttaggg atccattaat
    15181 cgttctcatc agcgtgccaa tgtctatctg tggtgcgctt atcccgctca acttgggagc
    15241 agccacaatt aatatctaca ctcaagtggg tttgattact ttgattggac tcatcagtaa
    15301 acatggaata ttgatagtag atttcgctaa tcatttgcaa cgtgaaaaaa atcttgataa
    15361 acgtgctgcc gttgaggaag ctgctggaat tagattacga cccatattaa tgactacagc
    15421 tgccatggtg tttggagtaa tacctcttct tattgccagt ggtgctggag cagcgagtcg
    15481 attcgacata gggctcgtta tctccacagg gttattaata ggtacctgtt tcacactgtt
    15541 cgtagtacct actttatata cctacttagc tgaagatcat cgacataagc caaacgaacc
    15601 agcagagatc cagtaattcg actttggatt ttttcgtaag ccttattaat ctttaaataa
    15661 ttcgtcaaac agcctgcatg tttcctcttc tccgaagcat gcagccgcac tttgtggata
    15721 ccagcaacaa gcaacggtca ttagagaatc ggccatttaa aactcaaagg actataatga
    15781 ttttttcctg agttcctgtt tttttaaaat ggctaaattc ccatactcta tgataaaatt
    15841 gtgagttttt accagtatag gctatacgat aatggacaaa acttactctc cggaagcaat
    15901 agaaaaagcc ctttataaaa aatgggaaag tcatcattac tttcaaccac gcggtgaggg
    15961 taaacgattt tgtattatgc ttccgcctcc taatgtaaca ggaagcctcc atatgggaca
    16021 tggttttcaa cacaccataa tggatgcatt aaccagatat catcgcatgc ttggtgacaa
    16081 aacattatgg caaccaggta ccgatcatgc cggcatctca actcaacttg ttgtagaacg
    16141 tcaactggaa gcccaaggca tatcgagaaa agatctcacc cgtgagcaat ttctggataa
    16201 agtgtggcaa tggaaagaag agtccggtaa taccatcacg caacaaatga ggcgtttagg
    16261 tgcttccgta gattggagtc gagaacgttt caccatggat gaaggattgt ctgctgctgt
    16321 acaaaaggta tttgtacagc tttatgaaga aggacttatt taccgaggaa cccgcctggt
    16381 gaattgggac cccaaactgg gcactgctgt ttcagatctt gaagttctct ctgaagaaga
    16441 agatggcttt ctttggcaca ttcgctatcc tgttgttgat tccgaagaat ttctaatagt
    16501 tgccactaca cgccctgaaa ccttactcgg tgattgtgcc gttgccgttc accccgatga
    16561 ctcacgtttc aggcacctta ttggaaagca ggttcatctg cccttatgtg atagaacaat
    16621 acccgtgatt gcggacgatt acgttgacaa ggaatttggc agtgggtgtg taaaaattac
    16681 ccccgcacat gattttaacg atcatgaagt aggcaaacgt catcaattac cacaaatcaa
    16741 tattttaaca aagaaaggga ctatcaataa aaacgctccc ttaaaatatc aggggatgga
    16801 tagatttgtt gcccgagagc aaatcatcaa agatttggaa caggaaggct tgctggcgaa
    16861 aacagaacct cacaaattaa aagtgcctcg aggtgaaaaa tcgaatgtca tcatcgaacc
    16921 tttattaact gatcaatggt atgttaaaac caaaccattg gcagaacctg ctatcgctgc
    16981 tgtaaagaaa ggagacatcc gttttattcc cgagacatgg gataaaacct atttccagtg
    17041 gatggataat attgaggact ggtgtatcag ccgccaacta tggtgggggc atagaattcc
    17101 tgcgtggtat gataatcatg gtaatattta tgtaggttat agtgaaaacg atgttcgctt
    17161 taaacataaa attgatcaat ctactcccct gaaacaagat gaagatgttc tggatacctg
    17221 gttttcatca gctctctggc ccttttcaac attaggctgg cctgagcgta ctcccgaatt
    17281 ggaacaattt taccctacat ccgtattggt aaccggcttt gatatcatat ttttctgggt
    17341 tgcccgtatg atcatgatgg gactaaagtt cactgggaaa attcctttta aagaagtctt
    17401 tataacgggt ttaattcgtg acagtgaagg gcacaaaatg tccaaatcca aaggaaatgt
    17461 actggatcca ttggacattg tagacggaat tgatctggat tctttaatag ccaaacgaac
    17521 gtctaacctg atgttaaatt ctgttcgtga cagaatcaca aaagccactc gtaaggaatt
    17581 tcctgaagga atcagcgctt atggaacaga cgcgctgcga ttcacttact gctctcttgc
    17641 ttcaacaggg cgtaatgtgc gttttgattt aggacgagtt gaaggatata gaaatttttg
    17701 caataaacta tggaacgcag ctcgctatgt cttattaaat acagacgaag agcaaattga
    17761 ttttggggat ggcgcatttc aatacagccc agctgatcaa tggatactat cccggctaca
    17821 aaataccgta agtaaggtac atcactattt tgaaacctat cggtttgatc tcctcgctaa
    17881 tacgctatat gaatttgtct ggcatgaata ttgtgactgg tatttagaat tatccaaacc
    17941 catattacag gatgaccagg ctttaagcgc catgaaaaga ggcacgcgaa gaactttaat
    18001 tcatgttctg gatcaaatat tgaagcttct tcatcctctg atgccattta ttacagaaga
    18061 gatttggcaa aaaactacaa aatttaccag tgaaaatggg attagtatca tgctgagcac
    18121 atatcccaaa gtcaatgagg aattcataaa cccctctata gaggaagaat tggattggtt
    18181 aaagtcagcc attcaatcac tacgaaccat acgcagtgaa atgtctatct cacccgccaa
    18241 gttaatacct ttatacatac gcaatattac tcctgaatta aaggagcgta tcgcaaaata
    18301 tgaaaaaatt ctcaagacgc taagcaagat agacaaaatt aattatcttg ctcctgatga
    18361 gaaagttcct gtttcagcta ctgcggtatt gggtgaaatt gagttgttga ttccaatggc
    18421 agatttgatt gataaggagg cagagttatc aagactgaat aaagagcttg ccaaattgaa
    18481 taaagacata gagctggctc agggtaaatt aaataatcct aaattcactg ataaagcgcc
    18541 tgaagaaatc attgctaaag aaaaagataa attagctcag gctcaactgg ctaaagataa
    18601 acttttgcag cataaaaaca ggattgagtc attataactc aatgatcctt agttaaaccc
    18661 tccttacagt catggctcaa cttaactcat tattctatac gtggctgtaa ggaacaaatg
    18721 gcacaaaccg gattatttaa aagaaaggat gtcttctcgt ggtaactcaa ttcaatacgc
    18781 ttaaatccta tattttcccc atcattttgc tattatctat tttacttggt gggttaacag
    18841 gatatgtttt taatcaaaac gttgcctggt taaaaccctt tggcgatatt tttcttaatt
    18901 tgatttttac taccatcgtt cccctaattt ttttcagtgt atcctcagcg attgcaagaa
    18961 ctggttctat tggcaaatta ggcaaaatat tctactcaat gacagtcata ttcttattta
    19021 caggaatagt tgctgcagtt tatgcccttt tcgtagtttt acttttccct ccagcgcaaa
    19081 acatgcctgt tctcttggaa agttcaaagc aactctctcc gacaaatctc ccggatcgct
    19141 tgatagagat ttttactgta cctgagtttt caaaattatt ctctcatgag catattctgg
    19201 cattagtcct tttttcaatc ctggtaggat tggctgtagc ccattccaaa gataaaggta
    19261 caatttttaa ttccttctta aaagcaggag aagacgtatt catgtcagta tttcgcctga
    19321 ttatgtatta tgctcctatc ggtttttttg cctattttgc agtaatagta agcgaaattg
    19381 gcccccaatt aatggaaaat tatttacgca ttgccatact ctactatacc gcttcagtgt
    19441 tctacttttt cttcgcgtta actgcctatg cttatttggc gggaaaatcc cacggtgtca
    19501 ggctattctg gagaaatatt ttccttcctg cagcaacttc gatagcaact tgtagtagtg
    19561 ccgccagtat tcctgccaat ttaatggcaa ccaaatccat gcacatccct gctgaaatct
    19621 atgaaacatc aataccctta ggctccataa ttcataaaga tggatcagtc atgggcggtg
    19681 tttttaaaat tgccttttta ttcgggatct ttcatttacc tttttccagc ttgcccgttc
    19741 ttataatggc attgggggtt tctttgttgg taggaacagt gatgggagcg attcctagtg
    19801 gcgggatgtt aggagaattg ttaattttgt cagtgtatgg ttttccacct tctgttttaa
    19861 ttgcagttgc tgccattagc atcatcattg atcctatagc gacaatggtc aacgtaacaa
    19921 gcaatagtgt ttgcagcatg atgattgcac gtctggtcga aggtaaaaat tggtttctca
    19981 gatcctacaa tgaaaataac ctataatcta aggtatgtgc aatataaaag catttcttgg
    20041 agggaatagt aatgaaaaaa atactgtatg ttattccggg tattttatta attacgaact
    20101 cttttgccct tgatatacct gagaatatca ccaattctaa caaagatctg ctcccttcac
    20161 cttttcctgt ttacgtcatt gaggggtctg ctgttgtcaa tcatccttac ccgggagcaa
    20221 aaaaagtact cctgccaacc gacaacagct atgtagatta tccaggatgc tatattgctt
    20281 gttattccca taatacaggt gcttacgcca tttccccaac aatttcagtc atgggacaaa
    20341 ttcgagtcaa aggacaatat gacgccagaa tttgccaacc tgatggatat aaaaatcaag
    20401 acattagtgc tgcttatcaa tttaaacagc tttgtgctga aaagattcct gcttgtcaaa
    20461 ataacagttg ttgggcgggt ggcgatactg gcgggtggtt tggaattcaa taagcagacc
    20521 aatgaatttc aaagaataaa atgagatagg atacttgatt ttgggtcaaa gccacgcctt
    20581 taaaattttt agcaagcatc atcactctaa acgtcagcag agaggtaatt cagcagtttg
    20641 aatagtgggg gatatggaga catttggtga atacctcata ttaattaaat tagtaaataa
    20701 ccagtgaacc aataacatac ctagacaggt tattatagtt aaagggattg gagttgtgat
    20761 caacataaaa aacccaatag gtaaaaaaac actattaaga attattttat ttaaatcttt
    20821 catacgagcg tttgtcattg ccaattgttc gttcttgatt tgttctcgac tagtctcatt
    20881 aagcttaagc tgatgtatta attgccgatc tttctctata tcgattaaaa cgcgtaaaag
    20941 tgagctaata agaatgatag aggatattac aggcgaaagc ggtactgcca cagctgaaaa
    21001 agaaaataat ccaaaagctg tcactacaga taagcttgtg agcaaggatc gaagaccatt
    21061 taattcttta ttctgccatt caattgctaa acgagatcgc tccatgccag tagcttgggt
    21121 gtattttaag tcgtattctc ttttgtcttg ttgataacga ataatcatca ctaaaacatc
    21181 gatgagttgc gccaatgttt ctaattgcat tccttgtagg cctgcagata ttgagtttct
    21241 ataagataac caaaaaaact gggtcagatt aacaacgcac cacaagctat catttaaaag
    21301 actgtagtat aaatccctac ggattttata attagttttt tcttgggatt cgactaaaat
    21361 gagatgcgct aataaaccaa cgttagctga aaatcgaagc atatacaaag aaaaactcat
    21421 cacacccaaa cccgcatcta agggagcaag atcccttgtg tttttttgtg attttgccaa
    21481 atactttgct aaacctttac cacgatttaa ttgaagtctt ttgtcattta aatcagtcag
    21541 tttgctatta aaaaacatta atgtggattt tataatcggt tttgctgtac ctgactcttt
    21601 tttgctcata ttagtatcat caaacaatta aatgaattaa taataagcaa ttaaatctgt
    21661 cagaagagtg acatcaagat tacaaaattg taatctaatt cattatggtg attagcattt
    21721 tcatgctaca attgagcata atttaaacac aacggcatta tgcgattatt aattgttgaa
    21781 gaccataaaa gaacagctga ttttatctgt aaaggattta atgaatatca atttcacgca
    21841 gatgctgtat acgatggtgc ggaagcaatc tatcgaatct cagaaattaa ctacgatgct
    21901 gttgttttag acgttatgtt acccataatg gatggatggg ctgtacttga aaagattcgt
    21961 gaactaaaac ctgatttacc cgttttaatg ctctctgcct gcgacgatgt cgatagtcgt
    22021 gtgaaagggc ttgaaatggg agcctatgat taccttgtta aacctttttc cttcaacgag
    22081 ctccttgccc gaataaaatc tttactacga agaaaaccca aagagaatcc tatcttatta
    22141 aagattgatg atttaactct aaacttacaa aagcatacag caatacgagg cgagaaaaag
    22201 ttgcctttaa cagcaaaaga attcatgtta ttgacgttta tgttgaaacg aaatggtgaa
    22261 gtactttcaa ggacagtcat tgctgaacaa gtttgggaca ttaattttga cacgaatacc
    22321 aatattattg atgtagcgat caagcgttta agggaaaaag tggataatga tcatcctact
    22381 aaattgatcc atactgtcag aggagtaggc tatgtattgg agattcgcta aatcaatgtc
    22441 tattactaac cgcttattac ttatatttat tggttcaacc gtactgatat tgtctattat
    22501 cacagggtta atctatcctc caatgaaaga atta
//
LOCUS       DBNCWK010000037        22390 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.037, whole genome shotgun sequence.
ACCESSION   DBNCWK010000037 DBNCWK010000000
VERSION     DBNCWK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22390
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.037"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            33..710
                     /locus_tag="AC06IO_001299"
     CDS             33..710
                     /locus_tag="AC06IO_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HIO9874499.1"
                     /translation="MNTLFTLISTVLLTSNITSSTPLFAKPGTDINTQLQHLSHKAPQ
                     LNKKVLKLALTAYKNASKRGAVKKPVLTVIDYSLPSNKQRMWVFDLRNERLLYNTYVA
                     HGKNSGVNVARSFSNRESSKQSSLGTYITKNTYIGHKGYSLNLQGLDRGFNDNAYNRR
                     VVIHGAWYVEPEFIKKAGRAGLSWGCPAIAQTLAKPVINTIKNGSVVFAYYPDKKFLS
                     HSGYLVA"
     gene            1136..1507
                     /locus_tag="AC06IO_001300"
     CDS             1136..1507
                     /locus_tag="AC06IO_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HIO9874500.1"
                     /translation="MIMNQQLNATKKDKLQVIDWLIENFPNAFFKKGNQVKPLKIGIF
                     DDLIDFYERLDTPPFSKKSLREALSYYSASPAYLSCQKPDTARVDIYGNEVDVVTPEQ
                     AKYAYQRYQERYGNKKSQELK"
     gene            complement(1535..2227)
                     /gene="ubiG"
                     /locus_tag="AC06IO_001301"
     CDS             complement(1535..2227)
                     /gene="ubiG"
                     /locus_tag="AC06IO_001301"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIO9874501.1"
                     /translation="MNQIESTIAIEEVHKFAQLANDWWDTNGPLRTLHDINGARFEFI
                     SEHINLKGLRVLDVGCGGGILCESMAKAGAYVSGLDAEPEAIQVAKEHAHKNQLQIDY
                     FCNPIEEYEDQGFDVITCMELLEHVQNPELVLQHCKRLLKPNGLLFLSTISRTLKAYL
                     GAIIAAEYVLNLLPRQTHDYDKFIKPSELVKMARLYDLNLIDMKGLCYNPFLRKTTLV
                     SDVSINYIMVLQ"
     gene            complement(2214..2933)
                     /locus_tag="AC06IO_001302"
     CDS             complement(2214..2933)
                     /locus_tag="AC06IO_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIO9874502.1"
                     /translation="MYENLLVGCHEEWKEILMKALNTMGSDYLKQIQEDGHWLPGLNQ
                     LFSAFSLPLSQTQYILLGESPYPRKISANGYAFWDNSVGLLWSATGLSKEVNRATSLR
                     NWIKTLLIARGDLHGNTSQEAIAKINKSCLVQTAEDFFKGMMKKGILLLNASLVYSKD
                     KVPYHARHWKPFMQNLFEQLAIRKPDVQLILFGKIAEKIPANKLLIGLVSEHPYNVSF
                     ITNQRVIEFFKPMDLLSYEPN"
     gene            3197..4882
                     /gene="legC3"
                     /locus_tag="AC06IO_001303"
                     /gene_synonym="ppeA"
     CDS             3197..4882
                     /gene="legC3"
                     /locus_tag="AC06IO_001303"
                     /gene_synonym="ppeA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector
                     LegC3/PpeA"
                     /protein_id="HIO9874503.1"
                     /translation="MIMFLANCNIEELVTEHIIQFLADEELSFAGLKDLILSKAPIPW
                     IHSSVTAALLKSRDSDKTEVKKNLEQQSYKAQMAEDKIQKEQDEAEAIKDKKLKEILT
                     RELNHIPTQISEQQTELRLLHYKLERLFESQTKVDVIQHPDSSMKKIKPSSSHSASIE
                     RLQRSINEREIKIQSLFEQEINNKIKLNEIEKRASVRSQHHTKRVKHAQARIGYNSTG
                     EDILSTLSGKNQSILLRSIQKQHHALEKKCSDLIQEADQINYPLFLEELQKHLNKKKH
                     NLSSQEVDALKSVIKYIKQHLEFEHEAINTQSSLHIKKQSISSQIVKLRELQNKLKTL
                     KNNNPHLTAANEELVSRNLELTAMKEHHANLRHRLGTPALLLFGLTFLFSIPLILTIS
                     GVIPFFIAPALLYILVSAPPTILLLSTLGVGIAAIVFSFKMHSNESAIKSNLQTIETN
                     SNQMNRNSQNLKSLEALTIPTLDMQIKKDENLRDQLMLSLQKQQRQASQAYQKAKEVE
                     CLSYVNSSFLNSGNTQSSDPSFSTHSEESDNALNDSNDSLEALEEETVNGIAS"
     gene            5018..6658
                     /gene="legC3"
                     /locus_tag="AC06IO_001304"
     CDS             5018..6658
                     /gene="legC3"
                     /locus_tag="AC06IO_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC3/PpeA"
                     /protein_id="HIO9874504.1"
                     /translation="MSLATYDVKQELVKLTQNFFSIDHQFSELKNLLTDKKTIPWIHN
                     QIVDQLSQIKKSDEIAMIARLEKSAYEQQMKEDDDEKNRDVEELRQDLLQRNHLTRQL
                     EILRVRKEQYERELLIRNTVPHVHTHPDTPVVHQHPSQPISAPLLNPNTHVHAHLEAT
                     LSNQDIELSVRKINRRISEITKELEILDSRNNAREIRRGDREKRLQARLNYVQKTAGV
                     INTLSPDNQKKLLSNIEKEKKSLTQQHSSLLLKADQLNYSIFLEQFELSLQIMQGPFQ
                     EVDALQTIVKRMKGHLNYKEKAASIQSRLDNTVLTIGENLRRLTRLNSQLSSLQLANP
                     DLTRRNERLEEQNRELLESYNSHIKTRNKLFFPTLVLSGLSLLFSIPLILTLTGIIPY
                     AIAPAVLLTLVIAPPALLLLAGLGVGIATITYAVKAYFNNSTIESNKETIESNRRQMG
                     TNQKEIYTLENQTIPNLKKELLQNEGIQNRLTDELQYFESLAEQALKQAKEVEPHTYP
                     YRPFFNSEVAVHEHPSVDNGIVTPSAPPLDPLYPTVRH"
     gene            complement(6747..8003)
                     /locus_tag="AC06IO_001305"
     CDS             complement(6747..8003)
                     /locus_tag="AC06IO_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIO9874505.1"
                     /translation="MFALVDCNNFYVSCERLFKPALANKPILVLSNNDGCVIARSNEV
                     KALGVQMGVPYFAVKALCKQHKVHVFSSNYTLYADMSRRVMSVLEDNWPAIEIYSIDE
                     AFLDLNTLPQSEHNEFCRKLQKIVLRYTGIPVSIGIGATKTLAKLANHIAKKELRTPV
                     FNIENQYHWLAKIVVGEVWGIGRQWGKKLVQQGIYTAHDLAGMDLQLIKKLFSVVLQR
                     TVLELRGVSCGSLQDFESRKSIISSKSFGSMQTEYSALAQAISSHCARAWEKLRHQKL
                     MACHLSIFIKTNRFRLDLPQYQQTIGFKLINPTDDLRHLIRCAKLCLRKIFRPGFHYQ
                     KVGVYLGDLIEKNCLQLDLFNQISEKELNQTERLMLVFDEINTKYGRHTLRLAAEGHV
                     KPWAMRAQSRSPCYTTSWSELPVVII"
     gene            complement(8003..8527)
                     /locus_tag="AC06IO_001306"
     CDS             complement(8003..8527)
                     /locus_tag="AC06IO_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIO9874506.1"
                     /translation="MSHGGARAGAGRPRGQGKYGESTKSVRIPESRIQDVMKYLQGEK
                     EFPAIPLYTSSVRAGFPSPGDDYIESMLDLNEYLIKHPASTFFVRASGESMINAGIYS
                     GDILIVDRGIEATHGKIVIVALNGELTVKRLSRQHGQVKLLAENPDFPPIDITEEYDM
                     VIWGVVTHVIHTVL"
     gene            8693..9916
                     /locus_tag="AC06IO_001307"
     CDS             8693..9916
                     /locus_tag="AC06IO_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIO9874507.1"
                     /translation="MVKLYKEIINSIDNHQATMVEQLHQFCEINSGTTNLTGLAVMAE
                     TLCSAFKPIADSTELIKLPPLSVIDMTGSTVTQSCGDALFISKRPHLKRRILLSGHMD
                     TVYSASNPFQKLSYLNANQINGPGVADMKGGLIVILHALAAFENTVFAEEMGWDVLIN
                     SDEEIGSPASGSLFKELAHRYQAALVYEPAMTPKGTLAKNRKGSGKLTLVATGRAAHA
                     GRAFDEGRNAICYLAEAVTAVHALNGQRNGVTINVGKIAGGEALNVVPDKAVAQLDIR
                     ISLPEDEIWVRNELNKIIRQLERPDYSLSVHGAFGRPVKRICAGTERLFHRIQKIGEV
                     LGLTIDWKDSGGCCDGNNLAHYGLPVLDTLGVRGGNIHSSDEYILLDSLSERAALSAL
                     LLIDLAQGGLEDLIK"
     gene            9913..10956
                     /locus_tag="AC06IO_001308"
     CDS             9913..10956
                     /locus_tag="AC06IO_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HIO9874508.1"
                     /translation="MMLFRSARDSDLDAIHQLAEISGVGMTTLPKDKELLKKRLEWSC
                     ESYQRNVTTPGCEYYFFVLEEQKNKTIVGTSAIEARTGYEIPFYSYKLSKRTRVCHTL
                     NIRSDYEVLSLVNDNQGRTEICTLFLHPDYRKNNNGLLLSKGRFLFMAQYPHRFAPIV
                     IAEMRGISDDKGNSPFWDNVGANFFHMPFVEADRLTLATNKQFIADLMPRNPIYVKLL
                     SPEAQAVIGKPHQSTIPAMNILLKEGFRYNNYVDIFDAGPTIESPLLEIKTISLSRIM
                     VIKNISDEASSSNRYLLANTQMDFRATISHAVFNERENTCIISKETAKLLDVKQGDSL
                     RISPIRTEDTPPL"
     gene            10970..12457
                     /gene="astD"
                     /locus_tag="AC06IO_001309"
     CDS             10970..12457
                     /gene="astD"
                     /locus_tag="AC06IO_001309"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HIO9874509.1"
                     /translation="MSKLQIIQSKGQYINGEWIKGNGLILESTNPASGTLLWQGNNAT
                     DEEIANACYVAHRALKSWANTSFEERARYTKAFVEQVEKNRDQLARLISLETGKPLWE
                     SQTEVSSVIGKVNLSIQAYQERTWPKQTETAEANACLRFKPHGVVVVLGAFNFPAHLS
                     NGHIVPALLAGNTVLYKPSEHTPAVAELIIQCWHDSGLPPGVINCLQGNANCGNTLLS
                     QDIQGVYFTGSYATGLRIHQQFCNRPEIILALEMGGNNPLVIDEVKDIDAAVYHTMLS
                     TMITAGQRCTCARRIIIPDSQTGDLFLERFAKACKLMRIGSFDSQPEPFIGPVISHVQ
                     ALKHLHAQKQLVEMGGEIILPMSLLVEYTGLISPGIIDMTRAKNPPDEEIFAPFAQIY
                     RYNHFDEAIQLANQTRYGLSAGLLSDNKDHYLQFYQHIRAGLINWNRPTTGAASSLPF
                     GGVGCSGNHRPSAYFAADYCAYPVASMEQPLLTTPAQRLPGLVLE"
     gene            12463..13809
                     /gene="astB"
                     /locus_tag="AC06IO_001310"
     CDS             12463..13809
                     /gene="astB"
                     /locus_tag="AC06IO_001310"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HIO9874510.1"
                     /translation="MNVYELNMDGLVGQTHHYAGLSSGNIASTNNALSISNPQAAARQ
                     GLEKMRRLYNMGLKQGLLPPHQRPNLNLLYQLGFKGTPSEQINKAYKTAPELLSACYS
                     ASCMWTANAATVSASVDTEDNKVHFTAANLISNLHRHQEADFSKKLLEFIFSNSDYFN
                     HHPLLPKSMGTSDEGAANHNRLCQSHAHSGINLFVYGKKVLGNHQFEQSPIKYPARQT
                     KEASEAIARNHLLNPERVIFACQNPLAIDQGVFHNDVISVANEHVFLVHEEAFYNQTY
                     VLDQLREKADFPLVIIQISKEQISVSEAVDTYLFNSQLITLPDQKNMILIAPVECQAN
                     LKVKTCIDRLVADPQNPINSVYYLDLKQSMRNGGGPACLRLRVPLNDYEFKAMHQGIL
                     IDNDLLDILDKWVLKYYRTELKISDLADPQLLYECLDALDELTQILKLGSIYPFQS"
     gene            13821..14546
                     /locus_tag="AC06IO_001311"
     CDS             13821..14546
                     /locus_tag="AC06IO_001311"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIO9874511.1"
                     /translation="MWKTKFGKCIYISPSGYKVYQNLLYRWLTLGSEAWQTVINRYIP
                     RRPGLHYLQMLTLMARKYPNDCCLLGLGGAGIAHLLGAQKTIRSIIAVESSEEVIQIA
                     QKFFMTDLIPHFTITHQNARDFVRENKKTFQHMLLDLYDATHFPDECHSMDFFIHCKK
                     QLTNDGFLAVNLANSQEQWALVQLIKRQFPSNIIIPIKKCANIVVIASKNDNKDLFIK
                     QVRNTNEIKKITWVKDWGYVAEY"
     gene            complement(14618..14983)
                     /locus_tag="AC06IO_001312"
     CDS             complement(14618..14983)
                     /locus_tag="AC06IO_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIO9874512.1"
                     /translation="MKRNALIIVLVYLTSNPIFAKNLGKYTYEIACKNCHAPNLAKAI
                     KAPPAFDKKAWKLRFKQAKIEAKNNPSQFESAMDYLLYNVKVGKGLMHHGGLCKEADV
                     PNTDCSDEALIAAINYMRK"
     gene            complement(15360..15917)
                     /gene="frr"
                     /locus_tag="AC06IO_001313"
     CDS             complement(15360..15917)
                     /gene="frr"
                     /locus_tag="AC06IO_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIO9874513.1"
                     /translation="MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDY
                     YGSPTPLSQVANITTSDSRTILVTPWEKSMVAAIEKAILNSDLGLNPATAGTAIRVPM
                     PPLTEERRKELIKVVRHEGEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAI
                     QKLTDRYISEVDAVLAEKEKDLMEI"
     gene            complement(15907..16650)
                     /gene="pyrH"
                     /locus_tag="AC06IO_001314"
     CDS             complement(15907..16650)
                     /gene="pyrH"
                     /locus_tag="AC06IO_001314"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIO9874514.1"
                     /translation="MMNESQPKLKYKRILLKFSGEALMGKSQFGIDPSVLDSLARDIA
                     ELIHMGVEVGLVLGGGNLFRGKALSQAGVGRVTGDHMGMLATVMNALALRDALERIDL
                     PARIMSAIPMLGVVDPYHRRKAITHLRNGQVVIFAAGTGNPFFTTDTAACLRAIEIGA
                     DIVLKATKVDGVYSADPLKNPDAKRYDYLTYKEVLTKGLEVMDSTAICLCQDQGMPLQ
                     VFDMAAPKALKRIVTGERVGTIVGANHDQ"
     gene            complement(16647..17525)
                     /gene="tsf"
                     /locus_tag="AC06IO_001315"
     CDS             complement(16647..17525)
                     /gene="tsf"
                     /locus_tag="AC06IO_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIO9874515.1"
                     /translation="MSTISAALVMQLRERTGAGMMECKKFLIATNGDIEQAIIEMRKA
                     GQAKADKKADRVAAEGIIVIARSSDERTAVMLEINSETDFVARDENFTNFANAVADVA
                     LTSLPKNIEDLSNQALSSGATVEQARQELVAKIGENIKLRRLEKMHCDGVIGYYLHGS
                     RIGVMVALKNGSEALAKDIAMHVAASKPMVVSRDQVPAEAIENEREIFTAQAKESGKP
                     QEIIDKMIDGRINKFIDEVSLLGQPYVKDPNIKVGQLLKEKNAEVISFVRYEVGEGIE
                     KKEDNFVEEVMAQVRT"
     gene            complement(17591..18355)
                     /gene="rpsB"
                     /locus_tag="AC06IO_001316"
     CDS             complement(17591..18355)
                     /gene="rpsB"
                     /locus_tag="AC06IO_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIO9874516.1"
                     /translation="MNNVSMRELLEAGAHFGHRTRFWNPKMSEYIFGSRNKIHIINLE
                     KTLPMLSDVTNYVSRLAANKAKILFVGTKRAAQDSIREHARRCGMPYVDHRWLGGMLT
                     NYKTVRQSIFRLKELKEMKEKGLFNDMIKKEALMLTRELEKLERSLGGIENMGGLPDA
                     LFVVDVGFEHIAVEEARRLRIPVIGVVDTNNSPDNIDYVIPGNDDSMRAVDIYVRCVA
                     DAILDGKNSNTVGRVSSDSEFVEVTSNSNEEEKSGE"
     gene            18689..19099
                     /locus_tag="AC06IO_001317"
     CDS             18689..19099
                     /locus_tag="AC06IO_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIO9874517.1"
                     /translation="MSKKSICLATLALIFTFGQPVFANSSEATQTKPCHCHDHKKILS
                     QLNLTQEQKTKIKTIKIQAQKSIKENYRQLKAIKEQINALIANEKLDEAKLDSLINQR
                     NKITGTMLKNRVMMKNQIYNQLTNEQKSQYKKNM"
     gene            19607..20065
                     /locus_tag="AC06IO_001318"
     CDS             19607..20065
                     /locus_tag="AC06IO_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1716 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874518.1"
                     /translation="MRGHMIFLSIPKGMEFKQITEKDNTNDYFVDPNGKLPRINIQAL
                     VKDALQYNKGRKKEISLPDFNIYRHKPPYRDELFLQYNPDHNGKYFTKESVNLVNGKE
                     FIKYKTPATSYGTFWFQKVQLSENRMDEVLAKRSEQRENRRHTGDSPNPT"
     gene            complement(20129..21817)
                     /locus_tag="AC06IO_001319"
     CDS             complement(20129..21817)
                     /locus_tag="AC06IO_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1717 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874519.1"
                     /translation="MPVRYSGFDYDGCVGVTNLINIVAQNPKLIQRIRETSNYYTKEV
                     ALIASNRQSDRDDKRNARSNGNGSCFIQIQKLCIDLGMEFDPFLLADAYNLVPAGTSL
                     HEATNPANGGKIQNGWLHDKSKTSLVYAHMQRASSLEAREQIDYFFYDDREDILEGLY
                     QFFSENPELIPSNVRMHLVKYACSRENVHTMDNYKIPVQGKGFTNPDYVKTIKAFGDK
                     AIENARDVYDIDGKEINAAGVRQAKYNCNFEFEANKLQPMKFVNNFNSPAELIKAHEQ
                     TIEKIKLDAFELYKAKINVITTLSNEQKVFLIRAKAQQWNVLCDEGQWNNPIFTLQAI
                     MAEKSDRFFLPQYAFTAKMLIKNYQEARENRKDGSRFWEQINAFIKSMNALKDPMRNA
                     QSIFEVFKKNSYSVKAVDGFVKKVVEVMNALMDKYPQRSTTEMVACMRILESNLQKMV
                     NFFSLVDRLITIYRGCTTAGPNFNKILTVVREALEEMKMITDSGDNYSTTLIDLDKVI
                     TAANNQLESLTYPKEYSTKNFHSQESYKVVGAYLCMQSQLVPKLDTKASTALAI"
     gene            complement(22208..>22390)
                     /locus_tag="AC06IO_001320"
     CDS             complement(22208..>22390)
                     /locus_tag="AC06IO_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkI/LegAS4"
                     /protein_id="HIO9874520.1"
                     /translation="NEGHNQFNINAFRKAIKDFNSNERSILLMQLRQSGLHLPEDLLE
                     QLGIMDSSITLESKFF"
BASE COUNT         7212 a         4582 c         3897 g         6699 t
ORIGIN      
        1 taatggtgat ttaacaagaa gaaaggagca gtatgaatac attatttact ctaatctcaa
       61 ctgtactact aacaagtaat atcacatcca gcactccatt atttgccaag cctggaacag
      121 acattaacac ccagttacaa catcttagcc acaaggcccc tcaattaaac aagaaagtat
      181 taaagcttgc tttaacggcc tataaaaatg cgagtaagag aggagcagtt aaaaagcctg
      241 tccttacagt aattgattat tccttgcctt caaataagca acgtatgtgg gtattcgatc
      301 ttcgtaacga acgtctatta tacaatacgt acgtagccca tggtaaaaat tcgggagtta
      361 atgttgcacg gagtttttct aacagagagt ccagcaaaca atccagctta ggtacctata
      421 tcacaaaaaa cacctatatc ggacacaaag gctactcctt gaacctccaa ggcctggata
      481 gaggctttaa tgataatgca tacaataggc gtgttgtgat acatggcgcg tggtatgttg
      541 aacctgaatt tattaaaaag gcaggtcgcg ctggtttatc ctggggctgc ccagctatag
      601 cacaaacctt ggcaaaaccg gtgataaata ccattaaaaa tggttcggtt gtttttgctt
      661 attaccctga taaaaagttt ttatctcact caggttatct tgtagcataa aaaagtaagc
      721 cagggactca gccctggctt ttcgcagtca tccgtataag aacttgcgtt cttattgtca
      781 tcctgacaaa tcagatccat ctaattgcaa atccatttgc acgactcata tccatataag
      841 tcttgattaa aatttagcaa ttgaatgagg gaagcgcaag gtgttaaatc acactttcac
      901 tcaatactat tttcaactca gaaatagcct tatatattta ttttattaat aatcaataag
      961 ttacttactt atgtaagaat tattgtcagg taaacagaat aatttcttct atggaattga
     1021 gaaatttctt acatcaatta cagcttaaat aacgtcattt tattttttag agcaacatgc
     1081 tgcttgatta tgtcataaga tatggtagtt tatttacgat gaaatgtagc ggggtatgat
     1141 tatgaaccaa caactaaatg caaccaagaa agataagctt caagtaatcg attggcttat
     1201 tgaaaatttt cccaatgcat tttttaaaaa aggaaaccag gtcaaaccgc tcaaaattgg
     1261 aatatttgat gatctaattg atttttatga gcgactggat actcctcctt ttagtaaaaa
     1321 atcgttaaga gaagcattaa gctattacag tgcttctcca gcttacttaa gctgtcaaaa
     1381 acctgataca gcacgtgtag atatttatgg taatgaagtc gatgtagtta cgccagagca
     1441 ggccaaatac gcttatcagc gttatcagga gcgctatggc aataaaaaaa gccaagagtt
     1501 aaaatagcac ggaaaagcca catttggatt tgttttattg taggaccata atgtaattaa
     1561 tgcttacatc agaaaccaga gtggtcttcc taaggaaggg attatagcat aggcctttca
     1621 tatcaatgag atttaaatcg taaaggcgtg ccattttaac taactcactg ggtttaatga
     1681 atttatcata atcatgtgtt tgtctgggta acaaattaag cacatactcc gcagcaataa
     1741 tagcgccaag ataagctttt aacgttctgc taatagttga taaaaataat agcccatttg
     1801 gttttaataa acgcttgcaa tgttgtaaaa ccaactcagg gttttgtacg tgttccaata
     1861 actccataca agttattaca tcaaaacctt gatcctcata ctcttcaata ggattgcaaa
     1921 aataatcaat ctgcaactgg tttttatgag catgttcttt ggcaacttga atagcttctg
     1981 gttctgcatc taatccactc acataagctc ctgccttggc catagattca cagagtatcc
     2041 cgccaccgca accgacatcc aacaccctca gcccctttag attaatgtgt tctgaaataa
     2101 actcaaaacg agcgccattg atatcatgca aagttctgag aggaccatta gtatcccacc
     2161 aatcatttgc aagttgggca aatttatgta cttcttcaat agcaatagtc gattcaattt
     2221 ggttcataag acaataaatc cattggttta aagaattcta taacacgttg attagtaata
     2281 aagcttacat tatatgggtg ttctgaaacc agtcctatca ataatttatt tgcgggtatt
     2341 ttctcagcaa ttttaccaaa taagattaat tgcacatcag gcttcctgat agccaattgc
     2401 tcaaataaat tttgcataaa tggcttccaa tgacgcgcgt gatacggaac cttatcttta
     2461 ctgtatacca gggaagcatt caataataaa attccctttt tcatcattcc tttaaaaaaa
     2521 tcttccgcag tttgtaccag acatgattta tttatcttgg cgatcgcttc ttgcgaggta
     2581 tttccatgca aatcaccgcg agcgatcaat aacgttttaa tccagttgcg cagtgaggtt
     2641 gctcgattaa cttccttact tagccctgtg gcgctccaca ataaacctac agaattatcc
     2701 cagaaagcat acccattagc agaaattttg cgtggatatg gagactcgcc taaaagaata
     2761 tattgtgtct ggctcaaagg cagactaaaa gcagaaaata actgatttag tccaggtaac
     2821 caatggccat cctcttgtat ttgttttaag taatcactcc ccattgtatt caatgctttc
     2881 atcagaatct ctttccattc ctcatggcat ccaaccaata aattctcata catcttaatt
     2941 tttacccttc cgtcacgata attcaatttt caaattgagg tattttgata attacctatg
     3001 tttttaaatc aggttttcag ccactttaac actattctta gaccatgaga aattgtctta
     3061 ttttaacatc tggaaataaa cccacgttgg ccaatagctt acctgttgat tgcagcgatt
     3121 ttcttgccac atccatcgtg gtgtgttgcc tgaatattca aattacacta acatcattta
     3181 aaaatgataa tctggagtga ttatgttttt ggccaactgc aatatagagg aattagttac
     3241 agaacatata atacaatttc tagcggatga agaattatct ttcgctggct taaaagattt
     3301 aattctgagt aaagccccaa ttccctggat tcattcctct gtaacagccg ctctgcttaa
     3361 atccagagac tccgataaaa cagaagtaaa aaaaaacctt gaacaacaat cttataaagc
     3421 tcaaatggct gaagacaaga ttcaaaaaga gcaggatgag gccgaagcaa taaaagataa
     3481 aaaattaaag gaaattttaa ctcgtgaatt aaaccacatc ccaactcaaa tcagtgaaca
     3541 gcaaacggaa ctcagattac ttcattacaa actagagcga ctctttgaaa gccaaactaa
     3601 agtcgatgtg attcaacatc ctgattcttc tatgaaaaag ataaagccgt cttctagtca
     3661 ctctgcgtcg attgagcgtc tgcaaagatc tattaatgaa cgtgagatta aaattcaatc
     3721 actttttgaa caggaaatta acaacaagat aaaattaaac gaaattgaaa agcgtgccag
     3781 cgtacgctca caacatcaca ctaaaagggt taaacacgct caggcaagaa ttggatacaa
     3841 tagtactgga gaggatattc tcagtacatt atcaggaaaa aatcaatcca ttttgttgcg
     3901 cagtattcaa aaacaacatc atgctcttga aaaaaaatgc agtgatctca ttcaggaagc
     3961 tgatcagatt aattacccat tatttcttga ggaacttcag aagcatttaa ataaaaaaaa
     4021 gcataactta tcttctcaag aggtcgatgc attaaagtct gttataaaat atataaagca
     4081 acacctggaa tttgagcatg aggcaatcaa tacacaatcc agtctccata ttaaaaagca
     4141 atccattagt tcccagattg taaaattacg agaattacaa aataaactaa aaaccttaaa
     4201 aaacaataac ccccatttga cagcggcaaa tgaagaatta gtttcacgta atttggaatt
     4261 aacggccatg aaagagcatc atgctaacct gcgtcatcgc ttgggtaccc ccgctttgct
     4321 tttatttgga ttaacgtttc tgttttcaat tcctcttatt ttaaccataa gcggagtaat
     4381 tccttttttc attgcgcctg cgctgcttta tattttggtc tcagcacctc caaccatcct
     4441 actgctatca actcttggtg ttggcatagc agctattgtt ttcagcttca aaatgcattc
     4501 caatgaatca gctattaaat ccaatttgca aacgattgaa acaaactcta atcaaatgaa
     4561 ccgaaattcc caaaatttaa aatcattgga agcgcttacc atccccactc tggatatgca
     4621 aatcaaaaaa gatgaaaatt taagagatca attaatgctt tctttacaaa aacagcaacg
     4681 tcaagcctcc caggcatacc agaaagcaaa agaggtagag tgcctatcct atgttaactc
     4741 ttcattttta aattccggca atacccaatc cagtgaccca tcattctcca cacattcaga
     4801 agagagcgac aacgcactaa atgactcgaa tgactctctg gaagcgctgg aagaagaaac
     4861 agttaatggg atagcgtcgt aaattgtctt tctatcaata aaatttcaat ggagcaaaac
     4921 tgagtcttaa ccccctgtaa taaaactgtt agtcccaata ttgataaatt agtctttatg
     4981 atctaaaatg caacaacttg attattggga gcaatctatg tcacttgcta cttatgatgt
     5041 aaaacaggaa ctcgtcaagc ttacccagaa ttttttctct attgatcatc aattttctga
     5101 attaaaaaac ttattaaccg ataaaaaaac cattccatgg attcataatc aaatagttga
     5161 tcagttatct caaattaaaa aatcagacga aatagccatg attgcgcggt tagaaaaaag
     5221 cgcttacgaa caacaaatga aagaagatga cgatgaaaaa aatcgtgatg ttgaagagtt
     5281 acgccaagat cttctccaaa ggaatcattt gacaaggcaa ctcgaaatct tgcgagtccg
     5341 caaagagcaa tatgaaagag agttgctcat ccgcaataca gtaccccatg tccatactca
     5401 tccggacact ccggtagttc atcaacatcc ttctcaacca atatctgcac ccttgctaaa
     5461 ccctaatact catgtacacg ctcatcttga agctacttta agtaaccagg atattgagct
     5521 gagtgttcgg aagattaaca gaagaatttc agagataacc aaagaattgg aaattctcga
     5581 ctccagaaat aatgcaagag aaattcgcag aggcgataga gaaaaacgcc ttcaagcacg
     5641 ccttaattat gttcaaaaaa cagcaggagt tatcaacact ctatcgcctg acaatcagaa
     5701 aaaactgtta tccaatattg agaaagaaaa aaaatcactc acacaacaac attcctcctt
     5761 gcttctgaaa gcagatcaat taaattactc catctttctt gagcaatttg aattgtcttt
     5821 gcaaattatg caaggtcctt ttcaagaagt agacgcattg caaacgattg taaaaaggat
     5881 gaaagggcat ttgaattaca aggaaaaagc ggcatcgatt caatcccgat tagataatac
     5941 ggtgcttact attggtgaaa atttaagaag attaacgcga cttaattctc aattgagttc
     6001 cttacagctt gctaatcctg atttaacaag gaggaatgag cgtctggaag agcaaaacag
     6061 agaactcctt gaatcgtata atagccatat aaaaacacga aataaactgt ttttcccgac
     6121 tctcgtttta tctggattgt ccttgctctt ttctattcca ttgatattaa ctctaactgg
     6181 aatcatccct tatgctattg caccagctgt attgttaact ttggttattg ctccccctgc
     6241 attgttattg cttgctggac ttggtgtcgg tattgcaaca atcacttatg ctgtgaaagc
     6301 ttacttcaat aattcaacga ttgaatccaa caaggaaaca atagaaagta ataggcggca
     6361 aatgggaaca aaccaaaagg aaatttacac tctggaaaat caaacaattc ctaatcttaa
     6421 aaaagagctg ttacaaaatg aaggaattca aaatcgccta actgatgaat tacagtattt
     6481 tgaaagcttg gccgagcaag cattaaagca ggcgaaagaa gtagagccac atacctaccc
     6541 atataggcca ttttttaact ccgaagttgc tgttcatgaa catccttctg tagacaatgg
     6601 tatagtaact cctagcgctc ccccgctcga tcccctctat cctaccgttc gacattaatt
     6661 tccagcctgg gttattaagc caggctgtca tccgggtgag tcttaatatg atttcccgga
     6721 tgacatagtg acatataagt ttctctttaa ataatgacca caggcaattc agaccaggaa
     6781 gtggtataac agggagaacg tgactgtgcc cgcatagccc atggcttcac atgcccttct
     6841 gctgccaaac gtaaggtatg acggccatac ttcgtattga tttcatcgaa tactaacatc
     6901 aatcgctccg tctgattcaa ttctttctct gaaatttggt taaacaaatc taactgcaaa
     6961 cagttttttt caattaaatc tcccaaatat acgcctacct tttggtaatg gaatccagga
     7021 cgaaatattt tacgcaggca caatttggca caacgaatta aatgtcgcag atcatcagtt
     7081 ggattaatca acttaaaccc tatagtttgc tgatattgtg gcaaatccaa acggaatcga
     7141 ttggttttga taaatatgga aagatggcat gccatcaatt tttgatgacg taacttttcc
     7201 caagctctgg cacaatggct gctgatggcc tgagcaagag cagaatactc tgtttgcata
     7261 gaaccaaagg atttggaaga tattatgctt tttcttgatt caaaatcctg taaactgcca
     7321 catgaaaccc cacgcaactc aagcactgtt cgttgcaata caacactaaa taatttttta
     7381 attaattgca aatccatacc agccaagtca tgagctgtat aaattccctg ttgtactaac
     7441 tttttacccc attgccgccc tatcccccat acctcgccaa caactatttt agctaaccaa
     7501 tgatattgat tttcaatgtt aaacacagga gttcttaatt cctttttcgc aatatggttt
     7561 gcgagtttgg ccagggtttt ggttgctcct atgcctatgg atacgggaat accggtatag
     7621 cgcaaaacaa ttttctgtaa cttgcggcaa aactcattat gttcagattg gggcaaagta
     7681 ttcaaatcaa gaaaagcttc atcaatggag taaatctcta ttgctggcca attgtcttca
     7741 agaactgaca taacccgacg tgacatatca gcatacaatg tgtaattgga tgaaaacaca
     7801 tgcactttat gctgcttgca taatgctttg actgcaaaat aaggtactcc catttgaaca
     7861 cccaaagctt ttacttcatt agatcgcgca atcacacaac catcattatt agacaagacc
     7921 aaaattggtt tattcgctaa ggctggttta aataaacgct cacacgagac ataaaaatta
     7981 ttacaatcga ccagcgcaaa catcacaata ccgtatgaat aacatgtgtg acgactcccc
     8041 aaatcaccat atcgtattcc tctgtaatat ctataggagg aaaatcaggg ttttcagcca
     8101 gcaacttaac ttgcccatgt tgacgggata aacgttttac tgtcaattcg ccattcaggg
     8161 caacgattac aatttttcca tgagtagcct ctatgccacg atcaacgatt aaaatatcac
     8221 cggagtaaat gccggcattt atcatagatt ctccagaagc tcgtacaaaa aatgtggatg
     8281 caggatgctt gatcaggtac tcattcaaat ccagcatgga ttcaatatag tcatcaccgg
     8341 gagaaggaaa accagctctt acagaactgg tatataaagg aatcgctggg aactcctttt
     8401 ctccttgaag atatttcatc acatcctgta tacgagattc aggtatacgg actgatttgg
     8461 ttgattctcc atatttacct tgcccacgag ggcgccctgc gcctgctcgt gcaccaccat
     8521 gagacatata ctcccccaaa cttgaattta gaatcaaaat tcaagtttaa ttttaaagta
     8581 gcccattgta aagttccgac aataatgaga atttcatatg acgactatac tgaattgacg
     8641 ccaatactca aattacgtta ttgttgggga tttagtgtaa gactaaagag ccatggttaa
     8701 attatataag gaaataatca attcaataga taatcatcag gccactatgg tggagcaact
     8761 ccatcaattt tgtgaaatca attctggaac aactaatttg acaggattag ccgtcatggc
     8821 tgagacattg tgttcagcat tcaaaccgat cgctgacagc acagaattaa taaaattacc
     8881 gcctttgtct gtaattgaca tgaccggaag cacggttacg caatcctgtg gcgatgcact
     8941 gttcattagc aaacgccccc acttaaaacg ccgaattctg ctaagcggtc atatggatac
     9001 ggtttattcc gccagcaacc cttttcaaaa attaagctac ctcaatgcta atcagatcaa
     9061 tggcccggga gttgcggata tgaaaggcgg gctaatcgtc atcttgcacg cattagcggc
     9121 ctttgaaaac accgtgtttg cggaagaaat gggttgggat gtgttaatta attcagatga
     9181 ggaaattggt tcgcctgcat caggttcatt attcaaagaa ttggcacatc gctatcaagc
     9241 cgctttggtt tatgaaccag caatgacacc taaaggtact ttggcaaaaa accgcaaagg
     9301 cagtggaaaa ctaacgctgg tagcaacagg aagagctgcc catgctggcc gggcatttga
     9361 tgaaggaaga aacgccatat gctatcttgc tgaagcagtt acggctgttc atgccttaaa
     9421 tgggcaaaga aatggagtaa caatcaatgt gggtaaaatc gctggtggag aagcactgaa
     9481 tgttgttcct gataaggcag tagcacaact ggatatccga attagtctac cagaagatga
     9541 aatttgggta aggaatgaat tgaataaaat tatcagacag ctagagcgac ctgattattc
     9601 tctaagtgtg catggcgctt ttggacggcc tgtgaagcgt atctgcgcag ggacagaacg
     9661 actttttcat cggattcaaa aaattggaga ggtgcttggc ttaactattg actggaaaga
     9721 cagtggaggt tgttgcgacg gtaataattt ggctcactat ggcttgcctg ttctggatac
     9781 tctaggtgtt cggggaggca atatacacag ttcagatgaa tatatattgc ttgatagctt
     9841 atcagagcgc gccgcactta gcgcgcttct tctgatagat ttggcacagg gtggtttaga
     9901 ggatctaata aaatgatgtt atttcgtagt gcccgcgact ctgacttgga tgcgatccat
     9961 caactggcag aaatcagtgg tgttgggatg acaacgctcc ctaaagataa agaattatta
    10021 aaaaaacgct tggaatggtc ttgcgaatcc tatcaaagaa atgtcactac ccctggctgt
    10081 gaatattatt tttttgtcct ggaagaacaa aaaaataaaa caattgtcgg tacctccgct
    10141 atagaagcaa gaacaggtta tgaaatacct ttttattctt ataaactctc taaaagaacc
    10201 agagtctgtc acacactcaa catacgcagt gattatgaag tattaagttt ggtcaatgac
    10261 aatcaaggtc gcacagaaat atgtactctg tttcttcatc ctgattacag aaaaaataac
    10321 aatggcctat tgctttcaaa aggacgcttt ttatttatgg cacaatatcc tcatcgcttc
    10381 gccccaattg tcattgccga aatgcgaggg atctctgacg acaaaggcaa ctcgccattc
    10441 tgggataatg ttggagctaa ttttttccat atgccttttg tagaagcgga tcgtctgaca
    10501 ctagccacga acaaacaatt tatcgccgat ttaatgcctc gcaaccctat ctatgtcaaa
    10561 ctcctttcac cagaggcaca agcagtcatt ggcaagccac atcaatcgac tatcccggcc
    10621 atgaatattc tcttaaaaga aggttttcgt tataacaact acgttgatat ttttgatgcc
    10681 ggacctacta tcgaatcacc attattagaa attaaaacta tttctttaag ccggatcatg
    10741 gttattaaaa acatcagtga tgaagcgagc agtagcaatc gctatttact cgccaatacc
    10801 caaatggatt ttcgagcaac aattagccac gctgttttta atgaacgaga aaatacctgc
    10861 attattagca aggaaacagc aaaattgctt gatgtaaaac aaggagacag tctacgtata
    10921 tcacccatac gtacagaaga tactccacca ttgtaaccaa ggaataatga tgtctaaatt
    10981 acaaatcata cagagtaaag gccaatatat taatggagaa tggataaagg gaaatggtct
    11041 cattctggag tctacaaacc ccgcctccgg aactttgctt tggcaaggga ataatgctac
    11101 agatgaagaa attgcaaacg cttgttacgt tgcacatcgc gcactgaaat cctgggctaa
    11161 taccagtttt gaggaaaggg ctcgttacac taaggctttt gtggaacaag tagaaaaaaa
    11221 tcgtgaccaa ctggcacgac ttatttcatt agaaacagga aagccattat gggaatctca
    11281 aactgaagtc agctcagtaa ttggcaaagt aaatttgtca attcaggctt atcaagaaag
    11341 aacctggcca aaacaaactg aaactgcaga agccaatgcc tgcctccgtt ttaaaccgca
    11401 tggtgttgtt gtagtattag gcgcgtttaa tttccctgcc catttaagca atggacacat
    11461 tgtccctgcc ctgcttgcag ggaacacggt attgtataaa ccaagtgaac ataccccagc
    11521 ggtagcagaa ttaatcatcc aatgttggca cgatagcggc ttgcctcccg gagttatcaa
    11581 ttgtctacag ggtaacgcaa attgtggaaa taccctattg tctcaagaca ttcaaggtgt
    11641 ctattttacc gggagttatg caacagggtt acgtatacac caacaattct gtaatagacc
    11701 cgaaattatt ttagctttgg aaatgggcgg aaataaccct ttagtcatcg atgaagtgaa
    11761 agacattgat gcggctgtat accatacaat gctttcaact atgatcactg cgggacaacg
    11821 ttgcacatgt gcaagacgaa ttataattcc tgactcccag acaggcgatc tatttttaga
    11881 acgttttgcc aaggcttgta aattaatgcg aattggatcc tttgatagtc agccagagcc
    11941 ttttattggg cctgttatta gccatgttca agcattaaaa catctacacg cgcagaagca
    12001 actagtcgaa atggggggtg aaatcattct gccgatgtcg cttcttgttg agtacacggg
    12061 actcatttct cctggcatta ttgatatgac tcgcgccaag aacccgccag atgaagagat
    12121 ttttgcgcca tttgcacaaa tttatcgtta taatcacttt gatgaggcaa ttcaattagc
    12181 gaatcagaca cgttatggtt tgtcagcagg cttactcagc gataataaag accactatct
    12241 gcagttttat caacatattc gtgccggact aatcaattgg aacagaccaa ccaccggtgc
    12301 tgccagtagc cttccatttg gtggtgtcgg atgcagtgga aatcatagac caagcgccta
    12361 ttttgctgct gattattgtg cttatcctgt agcaagcatg gagcaacctc tgttaacaac
    12421 tccggcgcag cgattaccag gtctggtatt ggagtaatta ttatgaatgt ttatgaattg
    12481 aatatggatg ggcttgttgg tcaaactcat cattacgcag ggctctcctc gggtaatata
    12541 gcttctacaa ataatgcttt atctatttcc aaccctcaag ctgcagcaag acaaggtttg
    12601 gaaaaaatgc ggcgacttta taacatgggg ctaaagcaag gcctattgcc cccacatcaa
    12661 cgacctaatt tgaatttact gtatcaattg ggatttaaag gcactcccag tgagcagata
    12721 aacaaagcct ataaaacagc gcccgaattg cttagtgcct gctactctgc ctcatgtatg
    12781 tggacagcta atgcagcaac tgtttctgca agcgtggata ctgaagacaa caaagtacat
    12841 ttcactgctg ccaatttaat cagcaatttg catcgtcacc aggaagctga tttttcaaaa
    12901 aaattattgg aatttatctt ttccaattct gattatttta atcatcaccc cctgttaccc
    12961 aaatcaatgg gcacaagcga tgagggagca gctaatcata atcgcttatg tcaaagtcat
    13021 gcgcactcag ggattaacct ttttgtatac ggtaaaaaag tgctgggaaa tcatcaattc
    13081 gaacagagcc caataaaata ccctgctcgg caaactaaag aggcgtcaga agccatcgct
    13141 cgcaaccatt tattaaatcc tgaacgagta atttttgcct gtcaaaatcc attagcaata
    13201 gatcaaggtg tatttcataa tgatgtcatc tcagttgcta atgaacatgt atttctggtt
    13261 catgaagaag ctttttataa tcagacttat gttttggatc aattaagaga aaaagccgat
    13321 tttcctttag taataattca gatctcaaaa gaacaaatca gcgttagtga agcagttgat
    13381 acctatcttt tcaattctca attgattacc ctgcctgatc agaagaatat gattttaata
    13441 gcccctgttg aatgccaggc aaaccttaaa gtaaaaactt gcattgatag attagtcgct
    13501 gatcctcaaa atcctattaa ctcagtgtat tacctggatc tgaaacaaag catgcgtaat
    13561 ggcggaggac cagcctgttt acgcttgcga gttcctttga atgactacga gtttaaagca
    13621 atgcatcaag gtattctaat tgataatgat ttgttagata ttttagataa atgggtttta
    13681 aaatattacc gaactgaact gaaaatatcc gatttagctg atccgcaact attatatgaa
    13741 tgtctggatg cattggatga attaactcag atcttgaaat taggctcaat atatcccttt
    13801 caatcttgag tatccgagtc atgtggaaaa caaaattcgg caaatgtatt tatatttcac
    13861 cgtcaggata taaagtctat caaaatttgt tgtaccgttg gcttacctta ggtagcgaag
    13921 catggcaaac tgtaattaac cgttatatac ctcgcaggcc tggtttacat tatctgcaaa
    13981 tgctgacact tatggcgcga aaatatccta atgattgttg tttattagga cttggaggag
    14041 caggaatagc tcacctactg ggtgcacaaa aaaccatacg ctctatcatt gccgtggaaa
    14101 gtagtgaaga agtgattcaa attgcccaaa aattttttat gacagattta attcctcatt
    14161 tcaccattac tcaccaaaac gcccgagatt ttgtacgtga aaacaaaaag acttttcagc
    14221 acatgcttct tgatctatat gacgcaacac attttcccga tgagtgccat tctatggatt
    14281 tcttcattca ttgtaaaaag caactaacta acgatggatt tttagctgtt aacttagcaa
    14341 attcccaaga acaatgggct ctggtccaat taatcaagag acaatttcca tccaatatca
    14401 ttattccaat aaaaaaatgt gccaatattg ttgtcattgc atccaaaaat gacaacaaag
    14461 acttgtttat taaacaagtc aggaatacta atgaaataaa aaaaattaca tgggtaaaag
    14521 attggggtta tgttgctgaa tattaattga aaggaaacaa taggttattt acaaatgaaa
    14581 ctcagccaaa agctggtatg tatttaaata ttttatctta ttttcgcatg taattaatcg
    14641 ctgcgattaa tgcctcatca gaacaatcgg tattagggac atctgcctct ttacataaac
    14701 cgccatgatg catcaaccct ttacccactt tgacattgta cagcaggtaa tccattgcac
    14761 tttcaaattg tgaaggatta ttttttgctt ctatttttgc ttgtttaaat ctcagcttcc
    14821 atgccttttt atcaaaagct ggaggggctt ttatagcctt ggctaaatta ggagcatggc
    14881 aatttttaca ggcaatttcg taagtatatt ttcctaaatt tttagcaaaa attggattac
    14941 ttgttaaata aaccaacact ataatcaatg cattcctttt catcacatcc ccaaattatt
    15001 attgctgaag caatcttagg attatatgag atattttgcc aattgaaata gttttttact
    15061 ttttaattaa agcgtataga ccattatctt gatgcttaat tatagagata acctaaccaa
    15121 tttattggta ctgaagagag ctaactaaaa atgaaaccaa tatgctaaaa gtcaaggcaa
    15181 gatcattcca attttagtcg attatgcccc gttatacttt ataaatttga gcatattttt
    15241 gtcattctcg cacggtcggg aatgacaaaa acttagcatt atataatcag agtatgtttc
    15301 ttcctgtact aaaaacaaga tctatagctc tattatataa aaggagtaac gcctacacct
    15361 taaatttcca ttaaatcttt ttctttctcg gctaatactg catcaacttc agaaatatat
    15421 ctatcagtca gcttttggat cgcttctgca gcacgtcgtt catcgtcttc agaaattgcc
    15481 ttttctttaa caagttcctt tagctgatta ttggcatccc ttctgatatt tcgaattgaa
    15541 actcttcctt gttccccttc atggcgaaca actttaatca actcctttct gcgctcttct
    15601 gttaaaggtg gcatcggaac tcgaatggct gttccagcag ttgcaggatt caaacccaaa
    15661 tcagagttta gtatagcctt ttcaatagct gcgaccatag atttttccca gggtgtaact
    15721 aaaatagttc gggagtcact ggtagtgata ttggcaacct ggcttaaagg tgtaggactt
    15781 ccataataat caaccatgac atgatctaat agactcgcat ttgccctgcc ggtacggatt
    15841 tttgacatat ccgtatgcaa agcctcaatg gtctttttca tgcgtttttc tgaatcttgc
    15901 ttaatctcat tgatcatgat tagctccgac tatagttcct actcgttctc cagttacaat
    15961 tcgttttaat gctttaggag ccgccatatc aaacacctgt aaaggcattc cctgatcttg
    16021 gcacaaacaa attgcagtgg aatccatcac ttccaaacct ttggttaaaa cttctttgta
    16081 agtcaaataa tcataacgct ttgcatcagg gtttttaagt gggtcagcag aataaactcc
    16141 atccaccttt gttgctttta aaacaatatc tgcgcctatc tcaattgccc tcaaacaagc
    16201 agcagtatct gttgtaaaaa agggatttcc tgttccagct gcaaaaatca ccacctgacc
    16261 attgcgcaag tgagtgatag cttttcgtct atggtatggg tcaactaccc caagcattgg
    16321 aatagctgac ataatacgag caggcagatc aattcgctcc aaagcatcac ggagtgccaa
    16381 agcattcata acagtggcca gcatccccat atggtctcct gtcactcgtc ctacacccgc
    16441 ctgagagagc gctttccctc tgaacagatt acctccacct aaaaccaaac caacttccac
    16501 acccatatga attaattctg ctatgtctcg agctaatgag tcaagaactg aaggatcaat
    16561 cccaaattga gatttcccca tcagcgcctc accactaaat ttaagcagaa tgcgtttgta
    16621 ttttaattta ggttgacttt cattcatcat gtacgaacct gagccatcac ttcttcaaca
    16681 aaattgtcct cttttttctc tattccttcc cctacttcat aacgaacaaa ggatattact
    16741 tcagcatttt tctcttttag aagttgaccc actttaatgt taggatcttt tacataaggt
    16801 tgtcctaaca agcttacctc atcaataaat ttattgattc gtccatcaat catcttgtca
    16861 atgatctctt gcggcttacc actctctttt gcttgagcag tgaatatttc tctttcgttt
    16921 tcaatcgctt cagctggtac ctgatcacga ctaaccacca tcggtttgct ggcagcaaca
    16981 tgcatggcaa tatctttagc caatgcttca cttccatttt ttaatgcgac cataactcct
    17041 atacgcgaac catgcaaata atacccgatt acaccgtcac aatgcatttt ttccaatcgg
    17101 cgtaatttaa tattttcacc aattttagca accagctcct gtcttgcttg ctcaacagta
    17161 gcaccagaag ataatgcctg attggacaag tcttctatgt tttttggaag gctggttaat
    17221 gcaacatcgg caacagcatt tgcaaaatta gtgaaattct catctcgtgc aacaaagtct
    17281 gtttcactat taatctcaag cattaccgct gtacgctcat cagaagaacg tgcaataaca
    17341 attatacctt ctgctgcaac tctatctgcc tttttgtctg cttttgcttg gccagcttta
    17401 cgcatttcaa tgattgcttg ctcgatatca ccgttagtag ctattagaaa ttttttacat
    17461 tccatcatac ctgcgcctgt gcgctcacgc aattgcatta ctaatgcagc actaattgtt
    17521 gacattttga tttctccaca tagcaaaaag ggggcttatt aaattaacgc cccctttatt
    17581 caaatagtaa ttattctccg gatttttctt cttcattaga atttgaagta acctcaacaa
    17641 actctgaatc agatgatact cttcctacag tattgctatt tttaccatct aaaatagcat
    17701 cagcgacaca gcgaacataa atgtccacag cacgcataga atcatcattg cctggtataa
    17761 cataatcaat gttgtcaggg ctattattgg tatcaacaac accaattacc ggaattctta
    17821 atcgacgcgc ttcttcaaca gcaatatgtt caaaaccaac atcaaccaca aataacgcat
    17881 caggtaggcc gcccatgttc tcgattccac caaggctacg ctccagtttt tcaagctctc
    17941 ttgtcagcat tagcgcttct ttcttaatca tgtcattgaa cagtcccttt tccttcattt
    18001 cttttaattc ttttaaacgg aatattgatt gacggactgt tttatagttg gtcagcatac
    18061 ctcctaacca tctatggtct acataaggca ttccgcatcg tcttgcatgc tctcgaatgc
    18121 tgtcttgagc ggctctttta gtgcctacaa aaagaatttt tgccttgttt gctgccaatc
    18181 ggctaacgta attcgttacg tcactcagca tgggtaatgt tttttcaagg ttaatgatat
    18241 ggattttatt tcttgagcca aaaatgtatt cactcatttt tggattccag aaacgtgttc
    18301 tgtgcccaaa atgagcacct gcttcgagca attcacgcat actaacatta ttcatatata
    18361 tatctccagg gttaagcctc cacgcttccc atacgaaacc caccaaggga ccctatcgca
    18421 tgtgacgaaa cgtgtgtgta tttaaagcgc ggtatttata acatattcat gccaattcag
    18481 caatcaaatt aacattgctc tctgtacatg acaaaaatat gtcatgtgca aagcattatg
    18541 tatgacgaca gtaattagac aaagcctatt aggaaataag tctatattaa tttgtacatt
    18601 ttgatttata ctgctatcag catggaagtt tgaaaaatgt tgtactcagg agaatcctat
    18661 taacgataac atttaagagg aaacgataat gagtaaaaaa tctatctgtt tagccacgct
    18721 cgctttgata tttacctttg gtcagcctgt ctttgccaac tcaagcgaag ctacacaaac
    18781 aaagccttgt cattgccatg atcacaaaaa aatactcagt cagcttaatt taactcaaga
    18841 gcaaaaaacc aaaatcaaaa caatcaaaat acaagcccaa aaatcaatca aagaaaatta
    18901 tagacaactt aaagcgatca aagaacaaat caatgcgctc attgcaaatg agaagcttga
    18961 tgaagccaag ctggatagtt taatcaacca aagaaataaa ataacaggga ctatgctaaa
    19021 aaatcgtgta atgatgaaaa atcaaattta caatcagcta acgaacgaac aaaaatcaca
    19081 atacaaaaag aatatgtaaa acttgattgg taccgataat aaaaaattcg gtaccaatct
    19141 atcccaactc ttcagatata atcattactg taattatgct ctttctcact gttgcttcct
    19201 gaacaatatt ttctgaacgc aaaaaaatgg gcaaattttc tctctaccat taaaaaacag
    19261 atgatatttc gctcagtatg ctaacccata gcatgctgca gcagctgcac tggcaatgat
    19321 gcaaagtgca aatattatag ttctcgccat attcttcttt gttttgggtt tcataacaaa
    19381 tcctgtaatg acggttatcc tgattgatat ttcatttaaa ttatagttca aattttatgc
    19441 acatccagac ccatatattg attgccctta cacagagtag catggagctt tactacaaat
    19501 tcattgcaaa acacttgcta caattattga cttatgaccc aatttagaac aacctatatc
    19561 ggcgatgagc aatattgttg ttgcagaata aaaatgaaac ccaattatga gaggacatat
    19621 gattttttta agtattccta aagggatgga atttaagcaa attacagaaa aagacaatac
    19681 gaacgactat tttgttgatc cgaatggaaa gttaccacga attaatattc aggctttagt
    19741 taaagacgct ctgcagtata acaaaggtag aaaaaaagaa atctccctgc ctgatttcaa
    19801 tatttatagg cacaaaccac cttatagaga tgaactcttc cttcagtaca atccagatca
    19861 taatggtaaa tactttacaa aagaatctgt aaatttggtg aatggtaaag aatttattaa
    19921 atataaaact cctgctactt catacggtac tttttggttt caaaaagtac aactatccga
    19981 aaacagaatg gatgaagtcc ttgccaagcg aagtgaacaa cgagaaaatc gcaggcatac
    20041 aggggatagc ccaaacccaa cataatcaaa caagaatagt gaaaaaggca tgtactagca
    20101 tgcccttttc aaaaagaaca aaatgaaatt aaatggccaa tgcagtagaa gctttggtgt
    20161 ctaattttgg aaccaactga ctttgcatgc acaaatatgc gcctacaact ttataagact
    20221 cttgtgaatg gaaatttttt gttgagtatt ctttaggata agtgaggctt tccagctgat
    20281 tatttgctgc ggttatcact ttgtctaaat caatcaaggt ggtagagtaa ttatcaccac
    20341 tgtctgttat cattttcatt tcttcaagag cctcccgtac aacagtcaga attttattaa
    20401 aatttggacc agcagtggtg caacctcgat agatagtaat taggcgatcg acaagagaaa
    20461 agaaattaac catcttttgc aaatttgact caagaatgcg catacatgct accatttctg
    20521 ttgtgctgcg ttgaggatat ttgtccatta aagcgttcat cacttcgaca acttttttca
    20581 caaacccatc tacagcttta acggaataac tgtttttctt aaatacttcg aaaatagatt
    20641 gagcatttct cattggatcc tttaaagcat tcatactttt aataaacgca ttaatctgct
    20701 cccaaaagcg gctgccatcc ttcctgtttt ctctagcttc ttgatagttt ttgattaaca
    20761 ttttcgcagt aaacgcatat tggggtaaga aaaagcgatc gcttttctcg gccattatcg
    20821 cctgcaaagt aaaaattgga ttgttccatt gtccttcatc acacaggaca ttccattgct
    20881 gggcttttgc tctgattaaa aagacttttt gttcatttga taacgtagtg ataacgttaa
    20941 tttttgcttt atacaattca aatgcatcaa gctttatttt ctctatagtc tgttcatgag
    21001 ctttaattaa ttcagcggga gaattaaaat tattaacaaa cttcattggt tgtaacttat
    21061 tggcttcaaa ttcaaaatta caattgtatt ttgcctgacg aactcctgca gcattaattt
    21121 cttttccatc tatgtcataa acatctcttg cattctcaat agctttatca ccaaacgctt
    21181 tgattgtttt gacatagtca ggattagtaa agccctttcc ctgaacagga attttgtaat
    21241 tgtccatggt atggacattt tcacggctac aggcatattt aactaaatgc attctaacgt
    21301 tagatggaat aagctctgga ttttctgaga aaaattgata caaaccttca agtatatctt
    21361 ctcgatcatc ataaaaaaaa taatcaatct gctctcgagc ttccaaggaa gaagctcttt
    21421 gcatatgcgc atacacaagc gaagttttcg atttatcatg taaccagccg ttttgaattt
    21481 ttccgccatt tgcaggattc gttgcctcat ggagactagt acctgctggc accaaattgt
    21541 atgcatcagc taataaaaac ggatcaaatt ccatgcctaa gtcgatgcac agtttttgaa
    21601 tctggataaa acaagaacca ttgccattgg agcgagcatt acgcttatca tcgcgatcgc
    21661 tttgccgatt cgaagcaatt agcgcgactt ctttagtata gtagtttgaa gtttctcgaa
    21721 ttctttggat aagctttgga ttttgtgcaa cgatatttat tagattagta acaccgacgc
    21781 aaccatcata atcaaaacct gaataacgaa ctggcatttt attttcctct caaaaagatt
    21841 gcataaccac tgtcacttta attaaggaga aaatttcaaa catggaagtc ggaacatcgc
    21901 cttgagtaaa gtgatacttt cttcatagcg acgcacccgt ttcctcatga aactagaaat
    21961 agccagtcct ctaggcaatc cttatagaga agacacatcg aaattttgct aaacaatatt
    22021 aagcaaagtt cataatgtta acattatgca aagatatgag tcaatttctt atttattgtt
    22081 gtttttctat tcatagttca aacagcccca atcaggttca atttggagtg tataagctct
    22141 catataacac gagttaatag agagctcgga atttctgata taagatatgt aaacagggaa
    22201 tatcaaatca aaaaaacttg ctttcaagtg tgatacttga gtccataatg cctaattgct
    22261 ctaacaagtc ttcaggcaaa tgcaaaccgc tctgccttag ctgcattaat aaaatactac
    22321 gctcatttga attaaaatct tttatcgctt ttctaaaagc attgatatta aattgattat
    22381 gcccttcatt
//
LOCUS       DBNCWK010000038        22215 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.038, whole genome shotgun sequence.
ACCESSION   DBNCWK010000038 DBNCWK010000000
VERSION     DBNCWK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22215
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.038"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            113..1003
                     /locus_tag="AC06IO_001321"
     CDS             113..1003
                     /locus_tag="AC06IO_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HIO9874521.1"
                     /translation="MPFAHLLLALLVVFVWGINFIFVKLSLEEFSPLLLCAVRFLLAS
                     VPAVFFIKPPAVPFKIIAGYGLIMFALQFALLFIGLRVGMTPGVASLLMQVQVFFSMF
                     FAIIFLGEQPQVGQIIGALIAFIGIGVVALHFDHNVSLMGFLCILAAAASWGIGNLIT
                     KKIHSVNLIAVIVWSSFVACLPMFILSLVFEGPESFVTAYEHMTWKGILSVLYIVYIS
                     TWVGYGVWNWLISRYPVGMVVPFTLLVPVVGILSSVLILGEPFYLWKLVAGLLVISGL
                     CINLLTTRLFVAKTQQPETA"
     gene            1171..1983
                     /locus_tag="AC06IO_001322"
     CDS             1171..1983
                     /locus_tag="AC06IO_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIO9874522.1"
                     /translation="MSDLKNIFLCADDFGLNHGITQGILKMARLQRLSAVSCIVNASS
                     FDVDAPDLLALKNGVRTGLHFNLTEGYFLSHPDKLCFGLKELLIRSHLGILNSRLISD
                     ELNTQLDHFIKVMGKLPDFIDGHQHIHQFPGIRRIIIDMYEQRLKGKSIFIRSTYPSV
                     NMSGYQAKTKILGITGGKILSSQLKKTNIPHNPYFAGIYDFSDRTNYRSLFRQWLNNV
                     PTNTLIMCHPGECENSDIIAAARARELNYFLSDEFMQDCEEYQVNLTTGPKS"
     gene            complement(1960..2349)
                     /locus_tag="AC06IO_001323"
     CDS             complement(1960..2349)
                     /locus_tag="AC06IO_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIO9874523.1"
                     /translation="MTTALKLSHRLIYFTGVGICAAIVHLLIVFNLVNFLNLQPLLSN
                     VIAFLIAFNVSYLGHKYFTFNQLQDKKQLSLPHFFLVAASAGLLNETLYYLLLRFTTL
                     HYITALVFVLSIVAVYNYLFSRFWACR"
     gene            complement(2333..3292)
                     /locus_tag="AC06IO_001324"
     CDS             complement(2333..3292)
                     /locus_tag="AC06IO_001324"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIO9874524.1"
                     /translation="MSKTAKHKKLISCVVPVFNEEVLIAEFIAALDKTLKSITYPYEI
                     LLIDDGSQDNTFAIIQTLRKEYSLRCIRFSRNFGKEKALSAGLDHARGDAVILLDSDF
                     QHPLELLSEFIAKWEDGYDMVYAVRKNRSDESWLKRVCAKIFYHLTSKINRINIPANA
                     GDFRLLDRKIVNALQTLPERNRFMKGLYSWVGFKQIAVPFNVHPRKSGSSQWSFYSLL
                     DLAITGITSFTAFPLRMISLGGMGVAALALIYAAWIVLSTLIFGIETPGWATIVTAIS
                     FFGGLQLFAIGVVGEYIGRVFDEVKHRPHYIIDEESSFDDHCS"
     gene            3438..4115
                     /locus_tag="AC06IO_001325"
     CDS             3438..4115
                     /locus_tag="AC06IO_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HIO9874525.1"
                     /translation="MNDSGWFLISTALLLGMRHGFDLDHLATIDAITRTVRYRPSLSR
                     MVGFLFSLGHGLVVIIVSLIIGGGTISASTPEWLDEVGNIVSVTCLLLFGFLTLWNTV
                     HYSSSAIIPTNLRSYLFRKLADNNVHPLMILLIGALFALSFDTISQIVLFSLAASALS
                     GFLFSGLLGFVFMLGMMISDGLNGFFVASLIRRADGFSLLFSRLVGFGIAFFSIGIAI
                     INCCKLL"
     gene            4127..4468
                     /gene="hypA"
                     /locus_tag="AC06IO_001326"
     CDS             4127..4468
                     /gene="hypA"
                     /locus_tag="AC06IO_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIO9874526.1"
                     /translation="MHELWLCKRIVEIIKQQATGNKCRKVKKVVLEIGQLVAVDKHAL
                     NFSFKVITQGTIAQNAKLSIVEIPGEAICNSCQQIVPMKQYYDECLICGNHSLTLTKG
                     EELKVKSMVVE"
     gene            4468..5226
                     /gene="hypB"
                     /locus_tag="AC06IO_001327"
     CDS             4468..5226
                     /gene="hypB"
                     /locus_tag="AC06IO_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIO9874527.1"
                     /translation="MCGICGCSVEKGQTMDNEHHAAHREHSVPHEHSVDIEQNILAQN
                     NEFAAHNRDYFLRHKILALNLMSSPGSGKTTLLSKTISDLGKEMDIAVIVGDQQTNCD
                     AQMLKSSGGYAWQINTGKVCHLDAHMISHAIQDISLKKQSLLFIENVGNLVCPASFHL
                     GEQFRIVVLSVTEGENKPLKYPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNP
                     SIDIITLSAVNGVGLKDWYDWLLHSFRLNVRASS"
     gene            5242..7467
                     /gene="hypF"
                     /locus_tag="AC06IO_001328"
     CDS             5242..7467
                     /gene="hypF"
                     /locus_tag="AC06IO_001328"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIO9874528.1"
                     /translation="MELERVQISIQGLVQGVGFRPCVYKLAKQMELTGFVQNNASGVL
                     IEIQGNYLSQFIPKLREQLPPLAAIHNLEFTSLPIVPNERAFEILDSQQGKINTVITP
                     DVCICSECLGELFDPQSRYYRYPFLNCTHCGPRFTITRNLPYDRCQTSMSQFPLCQSC
                     QSDYLNPDNRRYHAQPTACSQCGPQLALSVDKVAQRIQEGEIIALKGLGGYQLICDAR
                     NEETISKLRLRKNREAKPFALMVANVKSAEEIAEVSCQVRLVLESRERPIVLLRKKKA
                     PLPDGIAPGLNHFGIMLPSTPLHYLLFNSFLGNPDYCQWLDEYHSMVLVVTSANIGGE
                     PLIIDDKKAEQGLNSIADKIISHDREIITRTDDSVVRMINDTSVLIRRARGYVPAPLR
                     LPYEIPPTVALGGHLKNTFCITRGEEAFVSQHIGSLNNKATIEYFHESLNHLLKFLAV
                     KPERIAHDWHPDFYTSRLAQKYDIPVYGVQHHHAHLASVVAEHGIQEPVLGLVLDGYG
                     FGSDGEAWGGELLLLEKAEFKRVGHFFPIPQPGGELAAREPWRMAAGILHTLGRGDEI
                     SQRFKEQPQSPFLLQLLEKKIHCPLTTSCGRLFDAVSALLGVESLSLFEGHAAMRLES
                     MVTHPQVLNKGWTINGNVFDMRPTLQFVANTTEQQLGANLFHGTLIAGLSEWVTGICR
                     EKGIHMLLLSGGCFLNQVLAEGLINSLIKSGIKPLLPKLLPPNDGGIALGQAWIVGIK
                     E"
     gene            7477..7704
                     /locus_tag="AC06IO_001329"
     CDS             7477..7704
                     /locus_tag="AC06IO_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIO9874529.1"
                     /translation="MCLALPVQIDQVLEDNKAIVNLGGITKEISTVLLEQVSEGEYVI
                     IHAGFALTKLDEREANKTLSLFAQMLKDGLK"
     gene            7701..8807
                     /gene="hypD"
                     /locus_tag="AC06IO_001330"
     CDS             7701..8807
                     /gene="hypD"
                     /locus_tag="AC06IO_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIO9874530.1"
                     /translation="MKYIEEFRNGAYAQAIVNEIIATAEPQRHYQFMEFCGGHTHAIH
                     RYGIPSLLPNNVEMIHGPGCPVCVLPVARIDKAIALASMPQVILCSYADMLRVPGTGQ
                     RSLLTAKAGGADVRMIYSANDALKIAQENPDKDVVFFAIGFETTTPPTAFVIKQAKKL
                     NITNFSVFCNHVLTPIAMDSLLQANEVALDGFVGPAHVSIVIGSYPYEHVCKKYQKPI
                     VISGFEPLDVLHSILMLIQQLNEGRCEVEIQYTRAVNRTGNLLSQHIMTEILEIRDRF
                     EWRGLGFIPSSALKIKESFSEFDAEQRFELPDSAAQEHKQCLCGEVLRGVKKPTDCKL
                     FAKACLPENPLGSCMVSSEGACAAVYAYGRVNAL"
     gene            8804..9862
                     /gene="hypE"
                     /locus_tag="AC06IO_001331"
     CDS             8804..9862
                     /gene="hypE"
                     /locus_tag="AC06IO_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIO9874531.1"
                     /translation="MSEILITKNLTGPKLNFKEGTINLTHGSGGRAMAQLIEQLFLTA
                     FDNEWLAQKNDQACFIVPSGRMVIATDAHVISPLFFPGGDIGSLSVHGTVNDVAMSGA
                     KPLYLTASFILEEGFPLNDLRKIVYSMADAARQAAVAIIAGDTKVVERGKGDGIFITT
                     TGVGIVPEGIHISGDAAKPGDKIIVSGFIGDHGVAIMAHRNNLEFQTRVQSDTASLHE
                     LVGEMVKAVPDLHCLRDPTRGGLATTLNELASQSKVGFVIDESKIPIRTEVASACELL
                     GLDPLYVANEGKLIAICPSEKAERLLIAMKAHPLGLHAKIIGEVIEDHRCFVQLKTKL
                     GGLRIVDWLAGEQLPRIC"
     gene            9899..11038
                     /locus_tag="AC06IO_001332"
     CDS             9899..11038
                     /locus_tag="AC06IO_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIO9874532.1"
                     /translation="MNDKTASFLPHDKLQHLIDSLQNAGYSCVGPQVRDGAIVYDVLT
                     RAEQLPWGMRDHQEPGGYRLERIPERKAFAFANGPQGIKPLLFKPQETVWRVERDEQG
                     KLCFKPHQPDETPVAIIGSRACDLAAMSIQDKVFIESNKPDPRYKRRREQLFVVAVNC
                     TYSSANCFCVSAGTGPAVNNSFDILMTEIEDGFVVEAGSDKGKRIINQLDLHDAKSAQ
                     CTDAQNNVKQAAALQTKRIPLDNKRDLRDLLFSNLNHTRWDDVAERCLSCGNCTLVCP
                     TCFCHSEVEKPGLDGCNSEHHREWDSCFTTGHTYLNGKIIRDDTKKQYRQWLTHKVGS
                     WFDQFDTSGCVGCGRCVTWCPVGIDITEELAVISGESNVRKIESE"
     gene            11031..11876
                     /locus_tag="AC06IO_001333"
     CDS             11031..11876
                     /locus_tag="AC06IO_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIO9874533.1"
                     /translation="MNKSDYDPYLPREVEIVQRTQESSSIFTLHLRFTDEEHHKQFQF
                     YPGQFNMLYLYGVGEVAISIVSDPRKKTFLSHTIRAVGRVTKAMQKLQVGDRLGIRGP
                     FGVGWPLQKTIGKDIIVLTGGLGCAPSVSIINYILGRRRHYGKLSILQGVKHSEDFIF
                     RKQYAKWQKSDHTEVYIAADQAGPKWPWGVGYVTDLIDHIIIQPDNSVVMMCGPEMMM
                     NTAVKVFTQKGILENDIYLSMERNMECGIGHCGHCQYGGLFVCKDGPVFAYSQIKELF
                     KEPGF"
     gene            11894..12679
                     /locus_tag="AC06IO_001334"
     CDS             11894..12679
                     /locus_tag="AC06IO_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HIO9874534.1"
                     /translation="MKPRIAVHKFTSCDGCQLAFLNAGESLLTLSDLVEIVHFAEAGM
                     VDVDMKVDIAFVEGSISTPDEEHRIRKIRENSQYLITIGACATAGGIQALRNFADSRE
                     WIASVYASPDYIKTLTTSTAIAHHVKVDWELWGCPVNSGQILEAIRFLLSNATPKIKR
                     DSECMECKRKGNVCVLVTKKQPCMGPVTQTGCGSLCPTLGRACYACYGPKENANPNSL
                     GEWFLSIGLTKEAIARQFLHINNQAPAFNQAGNYFKGIKISNG"
     gene            12672..13964
                     /locus_tag="AC06IO_001335"
     CDS             12672..13964
                     /locus_tag="AC06IO_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIO9874535.1"
                     /translation="MGKDISINVPILARVEGEGALELCIRDSKIDTLKLKIYEPPRLF
                     EKFLEGRSYNEVLDFVARICGICPVAYQMSATQAIEQCFNLSPTPWVRDMRRLFYCGE
                     WLESHSLHVYFLALPDFLGFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHP
                     VGACVGGFYKAPSHSQIKLILDKAKDRVSDCEALIAWLASLSLPDNSHDFTSVSLYHP
                     TEYPMNEGRIVSDRGLNISKEEFDSYFKEYHVDYSNALHCLLQNKPYLVGPLARINNC
                     FGHLPVPIHLLLQSLSIQFPSRNMCHSIIARAVEMYYCVLEAIRILEQYDLPEQSRPE
                     IKPQAGEGAGCTEAPRGMLWQHYVFDETGQVLQARIVPPTSQNQARIEEDLKHSLIQF
                     GLNQEEEAIRYFSEMIIRNYDPCISCSTHFLNLKIDRI"
     gene            13961..14440
                     /locus_tag="AC06IO_001336"
     CDS             13961..14440
                     /locus_tag="AC06IO_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIO9874536.1"
                     /translation="MSLIKILGIGSPFGDDQVGCLAADCLKAELSKEQYISKSVCIES
                     HDRPGLRLIELMNSAKVAFLIDAVKSGCAIGTIHRLKNKEIYAFKNMLSTHEMGVAQA
                     LQLGHSLNALPEHIILYGIEIDSIAFNAMLSKPVEKAMRAVVIKIKKELKDMLNHIV"
     gene            complement(14469..15992)
                     /gene="sidD"
                     /locus_tag="AC06IO_001337"
     CDS             complement(14469..15992)
                     /gene="sidD"
                     /locus_tag="AC06IO_001337"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector deAMPylase SidD"
                     /protein_id="HIO9874537.1"
                     /translation="MVYYAIIKDIVFTYNLQFTHLIHNDRISEVNLGGVTMRSIITQI
                     CNGVLHGQTYQSGSNDLDKGNSEIFASSLFVHLNEQGKEIIKHKDSDDKIVIGYTKDG
                     MAFQIVVDGFYGCERQAVFSFIDNHVLPLIDNFSLDLARYPNSKNVTESFIHTIYSLR
                     SKHAPLAEFTMSLCVTYQKDDQLFCAGFGIGDTGIAIKRNEGTIEQLVCHTEVDGFKD
                     AFDNYSSTNIDSVIERNSVFNTKVMPGDELVGYTYVPPALEITEKEFEVETVDGKKIN
                     KRIVRHLNLDPGNFDDKDPLFSQLLQVVKSKQKQLVEQAKETGQIQRFGDDFTVGRLV
                     IPDQLLINQLRMHALSIRVSDGLLSYIKNENENKGFLGIYGFFTGADKNIEKATLYKN
                     LIAKYQNNPFTSLIILSALVSDSKTPVMTQYLVDYLDFPSKASLANKITALLLKELEN
                     PDMREILGSRLATDVIEELETKIIRYIHNPTGSDIHSTLNLWTADKIKAATNSSLTI"
     gene            16062..18005
                     /gene="sidM"
                     /locus_tag="AC06IO_001338"
                     /gene_synonym="drrA"
     CDS             16062..18005
                     /gene="sidM"
                     /locus_tag="AC06IO_001338"
                     /gene_synonym="drrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector guanine
                     nucleotide-exchange factor DrrA/SidM"
                     /protein_id="HIO9874538.1"
                     /translation="MSIMGRIKMSVNEEQFGSLYSDERDKPLLSPTAQKKFEEYQNKL
                     ANLSKIIRENEGNEVSPWQEWENGLRQIYKEMIYDAFDALGVEMPKDMEVHFAGSLAK
                     AQATEYSDLDAFVIVKNDEDIKKVKPVFDALNNLCQRIFTASNQIYPDPIGINPSRLI
                     GTPDDLFGMLKDGMVADVEATAMSILTSKPVLPRYELGEELRDKIKQEPSFSNMVSAK
                     KFYNKAIKDFTAPKEGAEVVSVKTHIMRPIDFMLMGLREEFNLYSEDGAHLSAPGTIR
                     LLREKNLLPEEQIARIESVYNQAMSKRFELHAEHKKEHDEMPYSDAKEMLDEVAKIRE
                     LGVQRVTRIENLENAKKLWDNANSMLEKGNISGYLKAANELHKFMKEKNLKEDDLRPE
                     LSDKTISPKGYAILQSLWGAASDYSRAAATLTESTVEPGLVSAVNKMSAFFMDCKLSP
                     NERATPDPDFEVGKSKILVGIMQFIKDVADPTSKIWMHNTKALMNHKIAAIQKLERSN
                     NVNDETLESVLSSKGENLSEYLSYKYATKDEGREHRYTASTENFKNVKEKYQQMRGDA
                     LKTEILADFKDKLAEATDEQSLKQIVAELKGKDEYKILAKGQGLTTQLLGLKTSSVSA
                     FEKMVEETRESIKSQERQTIKIK"
     gene            complement(18172..18891)
                     /locus_tag="AC06IO_001339"
     CDS             complement(18172..18891)
                     /locus_tag="AC06IO_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIO9874539.1"
                     /translation="MVDYHYFKSKLNHFYGRYVGYNQRPVFFDIDQTFPELNLITHHF
                     PDIKREFENVQANVKEFPRYHDIDPGETAISNSTEKNWNVFMLYLLGYQLKEARLLCP
                     TLCSLLEGIPHLIQAFFSILEPGKSIPLHEGPYIGYLRYHLGIHVPKNNPPQIFVNNQ
                     PYTWKEGEAVLFDDSWPHEVRNTSDDYRAVLIIDVLRPMPFLPQLVNQFVTHVIARYF
                     YGRKVMKRAMQYGGEVKPLAL"
     gene            complement(19030..20193)
                     /locus_tag="AC06IO_001340"
     CDS             complement(19030..20193)
                     /locus_tag="AC06IO_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874540.1"
                     /translation="MPSGILALGFVSMLTDLSSELIFSLLPVFLVSVLGTNALVIGLI
                     EGFAESIALLIRIFSGMYSDYLGKRKTLAIFGYSFSALSKPLFALATNIGMVFLARFL
                     DRIGKGMRGSPRDALIADIAPDNQYGAAYGLRQSLDTVGAIIGPLLALVLMIIWNNNF
                     QAVFWVAVIPGVLSVLVLILFVQEPVKPAGAKPINPIQPKEIKKLSHAYWYVVIIGAI
                     FTLARFSEAFLLLRAYQIGSPITWVPLILVVMYLMYSVSAYPFGKLADTMNHGKLLII
                     GLFILITADLILAFSHSLMFIYIGSCLWGLHMGATQGLLVAMVAGTAPDDLRGTAFGF
                     FNFICGIATLIASLLAGLLWDSFGSVFTFSIGALFCVVCLLGISLMPRIIAKT"
     gene            complement(20380..21312)
                     /locus_tag="AC06IO_001341"
     CDS             complement(20380..21312)
                     /locus_tag="AC06IO_001341"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIO9874541.1"
                     /translation="MSKQEHIISHNTDSSHLNTVMQQMKERIINEGDKPHVTVEAQLK
                     ILQELSQFRFGQFLIKNQGINGYWTHYMLTHPWFGRKTGLNNSREPLSNMECFLLDKA
                     PTMLATQQRFELFLQENQKALVNGATLASIPCGMLGELLYLDFNGINNIRLIGIDYDQ
                     AALEDANALAKERNPDRFTTLVCRNAWELNIQEEFDLISSNGLNIYEPDDNKVLDLYQ
                     QFHTALKKGGKLVTSFLTPPPHLSSQCEWIFNKINPEDLLLQRIIFSDILNAKFQCYR
                     SSQQTQKQLALAGFNNIHFLYDEAHLFPTAIAEK"
     gene            complement(21519..22157)
                     /locus_tag="AC06IO_001342"
     CDS             complement(21519..22157)
                     /locus_tag="AC06IO_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2461 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874542.1"
                     /translation="MDNRRKGMSQFNFFEKNQAFDENNFEQKLQDAINLIKQNQSFVA
                     RLVLDAINSNQVTVSTFFQLTPDHYQRMKTIMKKDYHTTLPKTFPPTMSAVRKIEKEL
                     DGIIYDNQHIYISSTKSVEEIAKTLIHEVCHFLNSDLYDKEMETEESKLVGYKDEVRA
                     FTAEKMFEKNKGYLLRSDINQIHETVTELYPEFTDPENPISGYIYSTFDSPS"
BASE COUNT         6639 a         3880 c         4748 g         6948 t
ORIGIN      
        1 gccaatatga cgaaattatt tgttgtttat ttgttcaatt attaagattt cttagcatat
       61 tctatgttta tgggttaaaa taaagagact tttataaaag ttgacttcag tcatgccttt
      121 tgcccatctc ttgttagctt tattggttgt ttttgtttgg ggaattaatt ttattttcgt
      181 caagcttagt cttgaagaat tttctccact gcttctttgc gctgtcagat ttcttttagc
      241 cagtgtcccg gctgtgtttt ttatcaagcc tcctgctgta ccttttaaaa ttattgctgg
      301 ttatgggtta atcatgtttg ccttgcaatt tgcattgctt tttattggtt tgcgtgtggg
      361 tatgactcca ggagtggctt ctttactcat gcaagtgcaa gtctttttta gtatgttttt
      421 tgcaataata tttttaggag aacaacccca agtcgggcag ataatagggg ctttgattgc
      481 atttataggc attggtgtag tggccttaca cttcgaccac aatgtgtctt taatggggtt
      541 tttatgtatt cttgctgcag cggcttcctg gggtattggc aacctcatta cgaaaaaaat
      601 tcattctgtt aatctgattg ctgtgattgt ttggagtagt tttgttgcct gtttgcccat
      661 gtttatactt tcactggttt ttgaagggcc tgaaagcttt gtaactgcct atgaacatat
      721 gacctggaaa ggcattcttt ccgtgcttta tattgtttat atttcaactt gggttggtta
      781 tggagtatgg aattggttga taagccgtta cccagttggc atggtagtac ctttcacttt
      841 attggttcca gttgtgggta ttttgagttc tgtccttatt ttgggagaac cattttatct
      901 ctggaagctg gttgccggat tgctggtgat tagtgggctt tgtattaatt tactgacaac
      961 tcgtctgttt gttgcaaaaa cgcaacaacc tgagacagcc tagttatccc cttccgattc
     1021 tatcatatcc tcttaagctc ataagtggag cagggagtta aatttctatc acccctataa
     1081 tatagattat cagataatta ttttggttat tacaaaataa ggataggggc agtaatatat
     1141 tacaattttt tttggaatta gaagttatta atgagtgatc tgaaaaatat attcttgtgc
     1201 gctgatgatt ttggccttaa tcatggtatt acccagggta ttttaaaaat ggctcgttta
     1261 cagcgcttat ccgcagtaag ctgtattgtg aatgccagtt cttttgatgt cgatgcccca
     1321 gacttattgg ctttgaaaaa tggggttcgc actggcttgc attttaatct cacagaaggt
     1381 tattttctat ctcatcctga caagctttgt tttggtttaa aagaattact tatcagatcg
     1441 catttgggta tcttgaattc aagattaatc tcagatgaat tgaatactca attggatcat
     1501 ttcataaaag tcatgggaaa gttgccggat tttattgatg gtcatcaaca tatccatcag
     1561 tttcctggta ttaggcgaat tattattgat atgtatgaac agcgattaaa gggaaagagt
     1621 atttttattc gttccactta cccgtcggtt aacatgtcag ggtatcaagc aaaaacaaaa
     1681 attctgggaa taactggcgg taaaatttta agtagccagt taaagaaaac aaatattcct
     1741 cataatcctt attttgcagg gatttacgat ttttctgaca gaacaaacta cagatcttta
     1801 ttcaggcaat ggttaaacaa tgttccgaca aatacgttaa tcatgtgcca tcctggtgaa
     1861 tgtgagaata gtgatattat tgcagcggca agagccaggg aactaaatta ttttttgagt
     1921 gatgaattta tgcaagattg tgaggaatac caagtcaatt taacgacagg cccaaaatct
     1981 tgaaaaaaga tagttgtaaa ctgcaacgat acttaaaaca aaaaccaggg cagtaatata
     2041 atgtaacgtt gtgaagcgta atagcagata atatagtgtt tcattaagta ggccggcaga
     2101 agctgccacc agaaaaaaat gaggcaaact taattgtttt ttatcttgta gctgattaaa
     2161 agtgaaatat ttatgaccta aatagctgac attgaatgca attaaaaaag caatgacatt
     2221 agacagcaga ggttgaagat ttagaaaatt gactaaattg aacacgatca aaagatgtac
     2281 gatagcagcg cagatcccta ctccagtaaa ataaattaat cgatgggata atttaagagc
     2341 agtggtcatc aaaactggac tcctcatcga taatgtagtg gggtctgtgt ttcacttcat
     2401 caaaaacacg gcctatgtat tcgccaacta caccgatagc aaataattgc aatccgccaa
     2461 aaaaactgat tgcagtgact atggttgccc agccaggggt ttcaatgcca aagattagcg
     2521 tgcttaatac aatccaggcg gcataaataa gcgccagggc tgccaccccc attcctccca
     2581 gtgaaatcat tcttagagga aaagcagtaa acgatgttat gccagtaatt gccaaatcca
     2641 gtaaagaata aaaactccat tgtgaactac ccgatttacg ggggtgtaca ttaaatggca
     2701 cggctatttg tttaaaaccg acccagctgt ataaaccctt cataaagcga ttacgttcgg
     2761 gaagggtttg tagtgcatta acaatttttc tatcaagcaa tctaaagtca cctgcatttg
     2821 ccgggatatt aatacggttt attttggaag tgagatgata aaaaatttta gcgcaaactc
     2881 gttttagcca tgattcgtca gaacgatttt tcctcacggc ataaaccatg tcataaccgt
     2941 cttcccattt ggcgataaat tcgcttaata attcaagcgg gtgttggaaa tcactatcca
     3001 ataagatcac agcatctccg cgcgcatgat ccaatcctgc actgagcgct ttttccttgc
     3061 cgaaatttct actgaaacga atgcaacgaa gagaatactc ttttcttaat gtttgaatga
     3121 ttgcgaaagt attatcctgg cttccatcat ctatcagtag aatttcataa ggataagtga
     3181 tgctcttaag agttttatcc aatgcggcga tgaattcagc aataagtact tcctcattaa
     3241 aaacaggaac aacgcaagag atgagttttt tatgttttgc agttttagac ataatgagta
     3301 ttaatgtaca agacaaaaga tagatagctt catatgttaa cctactcttg gaagttgagt
     3361 ccattgaggg ttataaaaac agtgaaagta ttggtatatt gttcacagtc tttaacatta
     3421 ataatggccc catactaatg aacgattcag gttggttttt gatttcaact gctcttttgc
     3481 ttggcatgcg tcatggtttt gatttggatc atctggcaac gatcgatgcg ataactcgca
     3541 cggttcgtta tagaccttct ttatcgcgca tggttggctt tttgttttca ctaggccatg
     3601 gtttggtagt tattattgtg agtttgatca ttggcggggg tactatttca gcgagtactc
     3661 ctgaatggtt agatgaagtg ggaaatattg tatcagttac ctgtttattg ttatttgggt
     3721 ttttaacttt gtggaatacg gttcattatt catcttctgc catcatacca accaacttaa
     3781 ggagttattt attcaggaag cttgctgata acaatgtaca tcctcttatg attcttctca
     3841 ttggtgcgtt gtttgcctta tcttttgata cgattagtca aattgtattg ttttcacttg
     3901 cagccagtgc tttatcaggt tttctatttt ccgggttatt aggttttgtt tttatgctgg
     3961 gaatgatgat atcggatgga ttaaatggat tttttgtagc aagcttgatt cggcgtgctg
     4021 atgggttctc attacttttc tcaagattag taggatttgg cattgctttt tttagcatag
     4081 gtattgctat aatcaattgc tgcaaattgc tttaaacagg ctaaaaatgc atgaattatg
     4141 gttatgtaag cgtattgtcg agattatcaa gcaacaagcc actggcaata aatgccgaaa
     4201 ggtaaagaaa gtagtactgg aaatagggca gttagttgca gtggataagc acgcattgaa
     4261 ttttagcttt aaagtgataa ctcaagggac gattgcacaa aatgcaaaat tatcgattgt
     4321 agaaattcct ggcgaagcaa tatgcaattc ttgtcaacaa attgtaccga tgaaacagta
     4381 ttatgatgag tgcttgattt gcggcaatca ttccttaaca ctgacaaaag gtgaagaatt
     4441 aaaagtgaaa tcaatggtgg tggaataatg tgcgggatat gtggatgttc agtagaaaaa
     4501 ggtcaaacca tggataatga gcatcatgct gctcaccgtg agcattcagt gcctcatgaa
     4561 cattcagttg atatcgagca gaatatttta gcgcaaaaca atgaatttgc tgctcataac
     4621 cgggattatt ttctcaggca taaaattttg gctttgaatt tgatgtcaag cccgggatcc
     4681 gggaagacaa cattactgtc caaaaccatt tctgatcttg gcaaggaaat ggatattgca
     4741 gtcatagtag gtgatcaaca aacaaattgt gatgctcaaa tgctaaaatc cagtggaggc
     4801 tatgcctggc aaattaatac tggaaaagta tgtcatttgg atgcccatat gatcagtcac
     4861 gccatacaag atatttccct aaaaaagcaa tcgcttttat ttatcgaaaa tgtgggtaat
     4921 ctcgtttgcc cggcttcatt tcatctgggt gagcaattca gaatagtggt tctttctgtg
     4981 acagaagggg agaataaacc gctcaaatat cctgatatgt ttcattcagc agatttgctc
     5041 ctgataacca aagcagattt attaccccat gtgaattttg atttggattt atgtaaggaa
     5101 tatgtacgcc gcacgaatcc atccatagac attattaccc tatcagcggt taatggtgtt
     5161 ggattgaagg attggtacga ttggttgttg cattctttta ggttgaatgt ccgggcttct
     5221 tcatagaaaa agtggtagcc catggaattg gaacgggtac aaattagcat tcaaggacta
     5281 gtgcaagggg ttggttttag accgtgtgtt tataagcttg ccaaacaaat ggaattaact
     5341 ggctttgtac aaaataatgc atcaggtgtt ttgatagaaa ttcaaggcaa ctatttatca
     5401 caatttattc cgaaattaag agagcaatta ccgcctctcg cagcaattca taacctagag
     5461 ttcacttcac tgccaattgt tccgaacgaa agagcttttg aaattcttga tagccagcag
     5521 ggtaaaataa atactgtcat tactccggat gtttgcattt gttctgaatg cctcggcgaa
     5581 ttatttgacc ctcaaagcag atactatcgt tatcctttct taaattgtac tcactgtggt
     5641 ccaaggttca caattaccag aaatctgcca tatgatcgtt gtcaaacatc aatgagccag
     5701 tttcctcttt gtcaatcgtg tcaatcggat tatttaaatc ctgataaccg tcgctatcac
     5761 gcgcaaccta cagcctgtag tcaatgtggc ccgcaactcg cactatcggt cgacaaggtg
     5821 gctcaaagaa ttcaggaggg ggaaattatt gctttaaagg gtttgggagg gtatcaatta
     5881 atttgtgatg ctcgaaatga agagactatt tcaaaattgc ggctaagaaa aaatcgtgag
     5941 gccaaaccat tcgctttgat ggtagccaat gtaaaaagtg ccgaagaaat tgccgaggta
     6001 agctgccagg tgagattggt attggaaagc agagaaaggc cgattgtctt gttaagaaaa
     6061 aagaaagcac ctttaccgga tgggattgct ccgggcttaa atcatttcgg tatcatgcta
     6121 ccttctactc cattacatta tttgcttttt aattcgttcc ttggcaaccc ggattattgc
     6181 caatggctgg atgagtatca ttccatggta ttagtggtga ccagtgcgaa cataggcggt
     6241 gaaccgctca ttattgatga taaaaaggct gagcaaggtt taaattctat agccgataaa
     6301 attatctctc atgatcggga aatcattact cgcacagatg actctgttgt tcgcatgatt
     6361 aatgacactt cagtattgat tcgtcgagca cgcggctatg tgcctgctcc tttaagattg
     6421 ccttatgaaa ttccccctac cgtggccttg ggaggacatc ttaaaaatac cttctgtatt
     6481 actcgtggcg aagaagcttt cgtttcacaa catattggca gtttaaacaa taaagcgacg
     6541 attgagtatt ttcatgagtc attgaatcat ttattaaaat ttcttgcggt caaaccagaa
     6601 cgaatcgctc atgattggca tcctgatttt tataccagta gattagcgca aaagtatgat
     6661 atacctgttt acggggtgca gcatcatcat gcccacttgg cttctgttgt agcagaacat
     6721 ggaattcagg agcctgtatt aggattggtt ctggatggct atgggtttgg ttctgatggc
     6781 gaagcttggg ggggcgaatt attattactg gagaaggcgg aatttaagcg cgttggacat
     6841 tttttcccta ttcctcagcc cggaggcgaa cttgctgcaa gagaaccctg gcgtatggct
     6901 gctggcattt tgcatacgtt gggaaggggg gatgagattt cgcaacggtt taaggagcag
     6961 cctcaatcgc catttttact gcaattattg gaaaaaaaga ttcattgtcc gctgactacc
     7021 agttgtgggc gactgtttga tgcggtcagc gcgcttttag gtgttgaatc gctctccttg
     7081 tttgaaggtc atgcggccat gagattggaa agcatggtga ctcatccaca agtcttaaac
     7141 aaaggctgga cgataaacgg caatgttttt gacatgcggc ctactttaca gtttgtagca
     7201 aacacgacag agcaacagtt aggggccaat ctgtttcatg gtactttaat agcaggtctt
     7261 agcgagtggg ttacaggaat ttgtcgagaa aaagggattc acatgctact attgagtggc
     7321 ggctgttttt taaaccaggt tcttgcggaa ggcttaatta attcattaat taaatcaggt
     7381 attaaacccc tgttgcccaa attgcttcca ccaaatgatg gtggaattgc gctaggtcaa
     7441 gcatggattg ttggaataaa agaataaggg tggcttatgt gcttagcgtt gccagtgcaa
     7501 attgatcagg ttttggaaga taacaaggca atcgttaatt tgggtgggat cactaaggaa
     7561 atttccactg ttcttttgga gcaggtttct gaaggagagt acgtcattat tcatgcagga
     7621 ttcgccttaa caaaactgga tgaaagagag gcaaacaaaa cattgagttt gtttgcccag
     7681 atgctaaagg atggcttgaa gtgaaataca tagaagagtt tcgtaacggt gcttacgcac
     7741 aagccattgt caatgaaatt atcgcgacag cagagccgca aagacattat cagtttatgg
     7801 agttttgtgg aggccatact catgctattc atcgttatgg gattccaagt ttgcttccca
     7861 acaatgttga aatgattcat ggccccggtt gcccggtttg tgttttgcct gttgcgagaa
     7921 ttgataaggc tattgcgctg gcctcgatgc ctcaagtaat actgtgcagc tacgctgaca
     7981 tgctgcgagt tccaggtact ggacaaagaa gtttgttaac cgccaaagca ggcggggctg
     8041 atgtcaggat gatttactct gcgaatgatg cattaaaaat cgctcaggaa aatcccgata
     8101 aagacgttgt attttttgct ataggctttg aaacaacaac ccctccgact gcttttgtca
     8161 tcaagcaagc taaaaaatta aacatcacta atttcagtgt attttgtaac catgtgttaa
     8221 ctcccattgc tatggatagt ttgctacagg ccaatgaagt tgccctggac ggctttgttg
     8281 gcccagccca tgtcagtatc gtgataggaa gctatcctta tgagcatgtg tgtaaaaaat
     8341 accaaaagcc gatagtgata tcagggtttg aacccttgga tgtgttgcat tctattctca
     8401 tgttaattca acaattgaat gaggggcggt gtgaagtgga aattcaatat actcgtgcag
     8461 tgaatcgtac gggtaatctt ttgtcgcaac acatcatgac tgaaatcctg gaaatacggg
     8521 atcgttttga atggcgtggt ttgggtttta ttccatccag cgcgttaaaa atcaaagagt
     8581 ctttttctga atttgatgct gagcagcgtt ttgaattacc tgatagtgca gcacaggaac
     8641 ataagcagtg tctttgtgga gaggtattaa gaggagtaaa aaagccaact gattgcaaat
     8701 tgtttgctaa ggcttgcctg ccagaaaatc ctttgggctc ctgtatggtt tcatcagaag
     8761 gagcttgcgc tgctgtttat gcttatgggc gggtgaatgc tttatgagtg aaatcctgat
     8821 aactaaaaac ttaactgggc caaaactgaa ttttaaagaa ggtactatca atttgaccca
     8881 tggcagtggt ggccgtgcta tggctcagct aatcgaacaa ttatttttaa ctgcgtttga
     8941 taacgagtgg ttggcacaga aaaatgatca ggcttgcttt attgttcctt caggacgtat
     9001 ggtcattgca accgatgccc atgttatttc cccccttttt tttcctggcg gggacattgg
     9061 ttctttgtca gtgcatggaa cggttaatga tgtggctatg tcaggggcaa aacctttgta
     9121 tttgacagcc agctttattc ttgaggaagg gtttcctctt aacgatttga gaaaaatcgt
     9181 ttattcgatg gccgatgcag ccaggcaagc agctgttgct atcattgctg gcgatacaaa
     9241 agttgtggag cggggaaaag gcgatggcat ttttattacg acaaccggag taggtattgt
     9301 ccctgaggga attcatattt caggtgacgc agcaaaacct ggtgacaaaa tcattgtgag
     9361 tggttttata ggagatcatg gggtagctat tatggcgcat cgcaataacc tggaatttca
     9421 gaccagagtg cagtctgata cagcttcctt gcatgagctt gtcggtgaga tggtgaaagc
     9481 ggttccagat cttcattgct tgcgtgatcc tacgcgtggc gggctcgcaa cgacactgaa
     9541 tgaattggcc tcacaatcca aagtcgggtt tgttattgat gaaagtaaaa ttccgattcg
     9601 taccgaggtt gctagcgcgt gcgaattatt aggtcttgat cctctttatg ttgctaatga
     9661 aggaaaatta attgcgattt gcccttctga aaaggcagag aggctattaa ttgctatgaa
     9721 agcgcacccc ttaggtttac atgctaaaat cattggcgaa gtgattgaag atcatcgctg
     9781 ttttgttcag ctgaaaacaa aattgggcgg gttacgaatt gtggattggc tggccggaga
     9841 gcaattaccc agaatctgtt aattaagtac taatggttga caagaaagga tgacgaggat
     9901 gaatgacaag acggccagtt ttttgcctca tgataaattg caacatttaa ttgattcttt
     9961 gcaaaatgcg ggatattcct gtgtgggacc tcaggtgcgt gatggtgcga ttgtttatga
    10021 tgtcctgacc agggctgagc aattaccatg gggtatgcgt gatcatcaag aaccaggtgg
    10081 ctatcgattg gaacgaattc ccgaacgcaa agcttttgca tttgccaatg gccctcaagg
    10141 aataaagcct ctattgttta agccacagga aacggtttgg agggttgagc gtgatgaaca
    10201 aggaaaattg tgctttaaac cacatcagcc agatgaaaca cctgttgcca ttatcggttc
    10261 tcgcgcttgt gatttagcgg cgatgagcat tcaagataaa gtttttatag aaagtaataa
    10321 gccggatcct cgttacaaaa gaaggcgcga gcaattattt gttgttgcag tgaattgtac
    10381 ctattcctct gcgaattgtt tttgtgtttc tgccggaact ggtcctgcag tgaacaattc
    10441 gtttgatatt cttatgacgg aaattgaaga tggatttgtg gtggaagcag gtagtgataa
    10501 aggtaaaagg attattaatc aactggattt acatgatgca aaaagtgctc aatgtactga
    10561 tgcccaaaat aatgtgaaac aagccgctgc cttgcaaacc aagaggattc ctctggacaa
    10621 taaaagggat ttgcgagatt tgctgttttc gaatttaaat catacccgtt gggatgacgt
    10681 ggccgaacga tgtttatcct gtggtaattg tactttggta tgcccaacct gtttttgtca
    10741 tagtgaagtt gaaaagccag gcctggatgg ttgtaacagt gaacaccata gagagtggga
    10801 ctcctgtttt acaacaggtc atacttatct caatgggaaa attattcgtg atgacacaaa
    10861 aaaacaatac aggcaatggc taactcacaa ggttggaagc tggtttgatc aatttgatac
    10921 cagcggttgt gttggatgtg gacgttgtgt gacctggtgt cctgtgggaa ttgatataac
    10981 tgaagagttg gcagtgattt cgggcgagtc taatgtaagg aaaatagaaa gtgaataaat
    11041 cagactatga tccttacttg ccaagagaag ttgaaattgt tcagcgtacg caggagtcgt
    11101 cttcaatttt taccctgcat ttgcgtttta ctgatgaaga acatcataag caatttcaat
    11161 tttatcctgg tcaattcaat atgctgtacc tttatggggt aggtgaagtg gctatttcaa
    11221 ttgtctcgga tcccagaaag aaaactttcc tgagtcacac tattcgagcg gtagggcggg
    11281 taaccaaagc catgcaaaaa ctgcaggttg gtgatcgtct tggaatacga ggtccttttg
    11341 gagtaggctg gccgctgcaa aaaacgatag gtaaagacat tattgtttta accgggggat
    11401 tgggttgtgc gccatctgtt tccattatca attatatttt aggaagacga cgtcattacg
    11461 gtaagctcag tattttgcag ggagtaaaac acagcgagga ttttattttt agaaagcagt
    11521 atgccaaatg gcaaaaatca gatcatactg aagtttatat agccgcagat caagctggtc
    11581 ctaaatggcc ttggggtgtt ggctatgtga ctgacttgat tgaccacatc attatacaac
    11641 cagataactc tgtggtgatg atgtgtggcc ccgaaatgat gatgaataca gctgttaaag
    11701 tgtttaccca aaaggggatt cttgaaaacg acatctatct cagtatggag agaaacatgg
    11761 agtgcggtat tggtcattgc ggtcattgtc aatacggtgg tttattcgta tgcaaagatg
    11821 gccctgtctt tgcttattcc cagattaaag agttattcaa agaacctgga ttttaaagtg
    11881 cctgagggat gctatgaaac cgcgtatcgc tgtacataaa tttacttctt gtgatggatg
    11941 tcaattggca tttttaaatg cgggtgaatc gttgttgact ttatcagact tggttgaaat
    12001 tgtgcatttt gcagaggcag gcatggtgga tgtggatatg aaagtggata tcgcatttgt
    12061 ggaaggcagt atttcaactc ctgatgaaga gcatcgaata cgtaaaattc gtgaaaacag
    12121 tcaataccta atcaccatag gtgcttgcgc tacagcgggc ggtatacaag ccttacgtaa
    12181 ttttgcagat tccagggagt ggatagccag cgtttatgcg tcaccggatt atatcaagac
    12241 tttaactacc tcaacggcta ttgcgcatca cgttaaagtg gattgggagt tatgggggtg
    12301 tcctgtaaat agcgggcaga tattggaggc gattcgtttt ttattatcta acgcgacccc
    12361 caaaataaaa agagattctg aatgcatgga atgcaaacga aaagggaatg tgtgtgtgct
    12421 ggtgaccaaa aaacaacctt gtatggggcc agtaactcaa acaggctgtg ggtcgctttg
    12481 ccccacttta ggcagagcat gttatgcttg ttatgggcca aaagaaaatg ctaatcccaa
    12541 ttctttggga gagtggtttt tatctatcgg attaaccaaa gaagcgattg ccaggcagtt
    12601 tttacatatt aataatcagg cacctgcatt taatcaagcc ggaaattact ttaagggaat
    12661 taaaatcagc aatgggtaaa gacatttcaa ttaatgttcc aattttggcg cgggtggaag
    12721 gagagggggc tcttgaactt tgcattcggg atagcaaaat tgacacatta aaattaaaaa
    12781 tttatgaacc cccaagatta tttgagaaat ttctggaagg tcgaagttac aatgaagtgt
    12841 tggattttgt agcccgtatt tgtggtatat gccctgttgc ttatcaaatg agtgctaccc
    12901 aggctataga acaatgtttt aacctaagtc ccactccttg ggttcgcgat atgcgtcgcc
    12961 tgttttattg tggcgagtgg ctggaaagtc atagcttgca cgtctatttt ttagctttac
    13021 ctgatttttt aggatttcaa tcagcacctg aaatggcaag gaaatatcca gaggaagtga
    13081 aatgcgggtt gcggattcag gcttttggta atgatttgat taaattgtta ggggggcgct
    13141 ccgttcatcc agtgggagct tgcgttggag gtttttataa agcgccttct cattcacaaa
    13201 tcaaattaat attagacaag gcaaaagata gggtttcgga ttgcgaagcg ttaattgctt
    13261 ggcttgccag cctatcttta cccgataatt ctcatgattt taccagtgtt tcgctttatc
    13321 accctactga atatcctatg aatgaaggaa gaattgtttc agatcgtgga ttaaacataa
    13381 gcaaagaaga atttgatagc tatttcaaag agtatcatgt ggattactcc aatgcattgc
    13441 actgtttatt gcaaaataaa ccttatttag tgggtcctct ggcccgtatt aataattgtt
    13501 ttggacattt gccagtaccc attcatcttc tgttacaaag cttgagtatt caatttcctt
    13561 cgcggaatat gtgccatagt attattgctc gagccgtgga gatgtactac tgtgttttgg
    13621 aagctatccg tattctggaa caatatgact tgccagagca atctcgtcct gaaatcaaac
    13681 ctcaggcagg agaaggagca ggttgtaccg aggctcccag aggtatgctg tggcagcatt
    13741 atgtttttga tgaaaccggt caggttttac aagcgcgtat tgttcctccc actagccaaa
    13801 atcaagcgcg tattgaggaa gatttaaagc attctttaat acaatttggc cttaatcagg
    13861 aagaagaagc gatcaggtat tttagtgaga tgatcattcg taattatgat ccgtgtattt
    13921 catgctctac acattttctg aatttaaaaa ttgatagaat atgagtctta taaaaatctt
    13981 aggtataggc tcaccttttg gtgatgatca ggtgggatgt ttggctgctg attgtcttaa
    14041 ggcagaattg tcaaaagagc aatacattag taaatcagtg tgcatagagt ctcacgatcg
    14101 gcctggtctt cgtttgattg aattgatgaa ttccgccaaa gtcgcctttt taattgatgc
    14161 tgtaaaatcg ggatgcgcca taggtacaat ccatcgctta aaaaataaag agatttatgc
    14221 atttaaaaat atgttatcga cccatgaaat gggggtggct caagctcttc aattaggcca
    14281 tagtcttaat gctctgccag agcatattat tttatatggg attgaaattg atagcatcgc
    14341 ttttaatgcg atgctgtcta aaccagttga aaaagcaatg agggcagttg ttatcaaaat
    14401 aaaaaaggaa ctcaaggata tgttaaatca tatcgtatga gtttcttttc ataatcaact
    14461 tataatactt aaatagtaag actcgagtta gttgcagcct taattttatc tgcagtccaa
    14521 aggtttagcg ttgaatgaat atccgagcct gttgggttat gaatataacg aataatttta
    14581 gtttctaatt cttctataac atcagttgct aacctggatc ccagtatttc tctcatatct
    14641 ggattttcta attcttttaa aagcaatgct gtaattttat tagcaagtga tgctttcgac
    14701 gggaaatcca gatagtcaac taagtattga gtcattacgg gtgttttact atcagagact
    14761 agagcagaaa gaataattag cgaggtaaaa ggattatttt ggtattttgc gatgagattt
    14821 ttataaagag ttgctttttc tatattttta tcagcccctg tgaagaaacc atatatccca
    14881 agaaaacctt tgttttcatt ttcattcttg atgtatgaaa gtaatccatc acttactctg
    14941 attgataaag catgcattct taattgattt attagtaact gatcaggaat gactaatctt
    15001 cctactgtaa aatcatcacc aaaacgttgg atttgtcctg tttcttttgc ttgctcaacc
    15061 agctgttttt gtttggattt tacaacctgt agcaattgag aaaataaggg atctttgtca
    15121 tcaaaatttc caggatcgag atttagatgt cttacaattc ttttgtttat tttcttacca
    15181 tcaacagttt ctacttcaaa ctctttttca gtaatttcta atgccggtgg aacataagtg
    15241 tatcccacca gctcatcacc aggcattact ttagtattaa agacagaatt tctttcaatg
    15301 accgagtcga tattggtgga tgaatagttg tcaaatgcgt ctttgaatcc atcaacttct
    15361 gtatgacaca ctaattgttc aattgttcct tcgtttcgtt tgatagctat tccggtgtca
    15421 ccaatgccaa atcctgcaca aaacagttga tcatcctttt gataagttac gcataaagac
    15481 atagtaaact ctgccaatgg agcatgtttg ctgcgcaaag agtaaatagt atgaatgaat
    15541 gattcagtaa cattttttga atttgggtat ctggcgagat ctaaggagaa gttatcaatt
    15601 aaagggagta catggttatc aataaatgaa aaaacagcct gacgttcgca tccataaaag
    15661 ccatccacta caatttgaaa agccattcca tctttggtat atccaatgac tattttatcg
    15721 tcagaatcct tgtgctttat aatttctttg ccctgttcat tcaaatgtac aaacaggctg
    15781 gatgcaaaga tttcactatt acccttatct aaatcattgg aaccagactg ataggtttgt
    15841 ccatgaagaa caccattaca gatttgtgta ataatcgaac gcatcgttac tcctcctaaa
    15901 tttacttcag atatcctgtc attgtgtatt agatgtgtaa attgtaaatt atatgtaaag
    15961 acaatatcct taatgatcgc ataatatacc aaatgatata cttaattaca agtcgagaga
    16021 tattatctat cttatctgaa gacttatgaa tcctcgagta catgagcata atggggagaa
    16081 ttaaaatgag tgttaatgaa gagcaatttg gtagtttata cagtgatgag cgagacaaac
    16141 cattactatc cccaaccgct cagaaaaaat ttgaagaata tcagaacaaa ttggcgaatc
    16201 taagtaaaat aattcgagaa aatgagggca atgaagtatc tccatggcag gagtgggaga
    16261 atggattacg tcaaatttac aaagaaatga tttatgatgc ttttgacgcg cttggtgtcg
    16321 agatgccgaa agatatggaa gtccattttg caggttctct tgccaaggca caagccactg
    16381 agtatagtga tttggatgcc tttgttattg ttaaaaatga cgaagatata aaaaaagtaa
    16441 aaccggtctt tgacgcatta aataatttat gtcagcgtat ttttactgca agcaatcaga
    16501 tatatcctga tcctattgga atcaatccaa gccgtcttat tggtacgcct gatgatttgt
    16561 ttggtatgtt aaaagacggg atggtagctg atgttgaagc aactgcaatg tcaattttaa
    16621 catcaaaacc tgttctacct cgctatgaat taggagaaga gttacgagat aaaatcaaac
    16681 aagaaccttc tttcagcaat atggtttcag ccaagaagtt ttataataaa gccattaaag
    16741 actttactgc acctaaagag ggtgcagaag tagtgagtgt caaaactcat attatgcgtc
    16801 ccatcgattt tatgctgatg ggattgcgtg aggaatttaa tttatacagc gaggatggag
    16861 cccatctttc agcaccagga actatacggt tattacgtga aaaaaattta ttgcccgaag
    16921 agcaaatcgc tcgaatcgag tcagtataca atcaagcgat gagcaagaga tttgagttgc
    16981 atgctgagca taaaaaagaa cacgatgaga tgccttattc tgatgctaaa gaaatgcttg
    17041 atgaggtagc caaaataaga gaactgggtg ttcaacgtgt cacaaggatt gaaaatctgg
    17101 aaaatgccaa gaagttgtgg gacaacgcga atagcatgct tgagaagggt aacatcagcg
    17161 gatatcttaa agcagcgaat gagcttcata aatttatgaa ggaaaaaaat cttaaggaag
    17221 atgatttaag gccagagttg agtgataaaa caatatctcc aaaaggatat gctattttac
    17281 aatctttatg gggagcagct tcggattata gtagagcggc tgcaactctc acagaatcaa
    17341 cagtagagcc tggtttagta tcagctgtta acaaaatgtc agcattcttt atggattgta
    17401 aattaagtcc aaatgagcga gcaacccctg atccagattt tgaggtaggg aaatctaaaa
    17461 ttttagtagg tataatgcaa ttcataaaag atgtagcaga cccaacttcg aaaatatgga
    17521 tgcataatac aaaagctttg atgaatcata aaattgctgc tattcaaaaa cttgagagaa
    17581 gcaataatgt taatgatgaa acattagaga gtgtgctgag ctctaaaggt gaaaatttat
    17641 ccgaatatct ttcttataaa tacgcaacaa aagatgaagg aagagagcac cgctatacgg
    17701 caagcacgga aaattttaaa aatgttaaag aaaaatatca gcaaatgcga ggtgatgctt
    17761 taaaaacaga aattctggct gatttcaagg ataaactggc tgaagctacc gatgaacaga
    17821 gccttaagca aattgttgct gaattgaaag gtaaagatga atataaaatt ttggctaagg
    17881 ggcaagggtt aacaacccaa cttttagggc taaaaacaag ttccgtgtcc gcatttgaga
    17941 aaatggttga ggaaacaaga gaaagtatta agtctcaaga aagacaaacc attaagataa
    18001 aataactagc gcatttcaat tatgaagata tgcttatatc gctcttttta gtatagagaa
    18061 agactgataa gagctaattc aataagggtt gaacctaaag ttcaactttt atttttttat
    18121 tgattgataa atattttaaa cgattttcga aagataataa tcgttgttta cttaaagtgc
    18181 caaaggcttt acttctccac catactgcat cgcgcgtttc attactttgc gaccataaaa
    18241 atatcgagca atgacgtggg ttacaaattg attgaccagt tgcggcaaga aaggcatagg
    18301 gcgcaagaca tcaatgatta atacggcacg ataatcatca cttgtatttc ttacttcatg
    18361 tggccaggag tcatcaaaaa gtactgcttc tccctctttc caagtgtaag gctggttatt
    18421 aacaaagatt tgtggtggat tgtttttagg gacatgaatt cctaaatgat atcttagata
    18481 gccaatatag gggccctcgt gtagaggaat tgattttcca ggttctaaaa tcgagaaaaa
    18541 agcttgtata agatgtggta ttccttccaa taaactacaa agtgtcgggc ataataaacg
    18601 ggcttccttg agttgataac caagaagata cagcatgaat acattccagt ttttttctgt
    18661 ggagtttgat atggcggttt ctccaggatc tatgtcgtgg taacgtggaa attctttcac
    18721 attcgcttgt acattttcga attccctttt gatatcagga aaatgatgtg ttatgagatt
    18781 taattctggg aaagtttgat caatatcaaa aaaaaccggg cgttgattat atcctacata
    18841 gcgcccataa aagtgattca atttactctt gaaataatga tagtctacca ttgcttattc
    18901 ctttatttta tttgtgataa taatcaggtg ccgaatgttc gactatcctt gggaaaatca
    18961 gctattttaa aatgaagttt gaagaaatgg aagtttaaaa aatggtcgtt tttataccaa
    19021 ggccgttttt caggtttttg caatgattct tggcattaaa cttatcccca ataagcatac
    19081 aacacaaaat aatgctccga ttgagaacgt aaagactgat ccaaaactgt cccagagcaa
    19141 tcctgcaagc aggcttgcaa taagtgtagc aatcccacat atgaaattaa aaaagccaaa
    19201 agctgtacca cgcaaatcat caggggcagt tcctgcaacc atggctacta aaagaccctg
    19261 ggttgcaccc atatgcagac cccatagaca ggatccaata taaataaaca ttagtgaatg
    19321 actaaatgcc aatatcaaat cagctgtaat taaaataaat aaccctataa tcaagagctt
    19381 gccatggttc atagtatcag ccagttttcc aaaagggtat gcgctcaccg aatacatcag
    19441 gtacatgaca accaggatta agggtaccca tgtaataggt gaaccaatct gatatgctcg
    19501 taaaagaaga aaagcctcgc taaagcgtgc cagagtgaaa atggcgccaa ttataaccac
    19561 ataccaataa gcatgactta atttctttat ctctttcggt tgaattggat taattggttt
    19621 tgcaccggca ggttttactg gctcttgcac aaacaaaata agaaccagca cagacaatac
    19681 tcctggtata actgccaccc aaaacacagc ctgaaagtta ttgttccaga ttatcattag
    19741 caccagagcc aaaagaggcc ctataatagc acccacagtg tcaagagatt gccgtaaacc
    19801 ataggctgcg ccatattggt tgtctggcgc tatatcagca ataagtgcat ctctgggaga
    19861 acctctcatt cctttcccta ttctatccag aaaacgggca aggaaaacca tgccaatatt
    19921 agtagccaaa gcaaatagcg gtttgctcaa cgcgctgaaa ctataaccaa atattgccaa
    19981 tgtttttctt ttacccagat aatcactgta catcccagag aagattctga ttaagagcgc
    20041 aatggattct gcaaaaccct cgattaaccc gatgactaat gcatttgtgc ccaatactga
    20101 aaccaaaaaa acaggcaata agctaaaaat aagttctgaa gaaagatccg ttagcatact
    20161 gacaaatcca agggcaagga tgcctgatgg cattgaagtc attccatttt ttttcttcct
    20221 gttgatgctc atttttccct gtaaaagacg atgactaatt tatcgggggt tggttaagtc
    20281 cgatataggc tttttatcat gttgccacat tctttattta aggttaaata ctactgtcgc
    20341 tgtggttaaa aaatattgat aacaatttcc tttttcttgc tatttttcag ctattgcggt
    20401 tgggaagaga tgggcttcat catatagaaa atgaatatta ttgaagcctg ccaatgcgag
    20461 ttgcttctgg gtttgttgtg atgagcgata acactggaat tttgcattta aaatatcgga
    20521 aaaaataatt ctctgcagta ataaatcttc tgggttaatt ttattgaata tccattcgca
    20581 ttgtgaactc aggtgagggg gaggagttaa aaaactggtt actaatttcc cgcctttttt
    20641 taaggccgta tgaaactgtt ggtataaatc cagaactttg ttatcgtctg gttcataaat
    20701 atttaaccca ttgctggaaa tgagatcaaa ttcctcttga atattaagct cccaggcatt
    20761 acgacaaaca agcgttgtga atctgtccgg gtttcgttct tttgccaagg cattggcatc
    20821 ctctaatgct gcctggtcat aatctatacc tatcagtcgg atgttgttga tgccattgaa
    20881 atcaagataa agtaattccc ctaacatccc acaggggata gaagcaaggg ttgcgccatt
    20941 aaccagagct ttttgatttt cctgcaaaaa taattcgaaa cgttgttggg ttgctaacat
    21001 tgttggtgct ttgtccaaca agaaacattc catgtttgat aatggctcgc gactattgtt
    21061 taaccccgtc tttcttccaa accaaggatg agtcaacata taatgagtcc aataaccatt
    21121 gataccctga ttttttatta gaaattgacc aaacctaaat tggcttaatt cttgaagaat
    21181 cttaagttga gcttctaccg tgacatgtgg cttatcaccc tcgtttatta ttctttcctt
    21241 catttgctgc attacagtat tcagatgaga tgaatcggtg ttatgagaaa tgatatgttc
    21301 ttgttttgac atacaggctc tctggataat ctttataatt attaactgct gtgagtttaa
    21361 gataagaaaa tggatttatt ttataattca aactcagagc aattatactt aatattcttg
    21421 aagatacgta gattttttaa ctcatttcat tgtacgttcc actacggcga tcgaaaatca
    21481 aggcttttcg actcaagctg gcaaaatgta aacagccctt aagaagggct atcaaaagta
    21541 ctgtaaatat accctgatat tgggttttcc ggatcggtga attcaggata tagttcggta
    21601 accgtttcgt gtatttggtt tatatcgctt ctgagcaggt agcctttatt tttttcaaac
    21661 attttttctg ctgtaaatgc ccttacttca tctttatagc cgactagttt tgactcttct
    21721 gtttccattt ctttatcgta aagatctgaa tttaaaaaat gacagacttc atggattaat
    21781 gttttggcaa tttcttcaac gctttttgtc gaactgatat aaatatgttg attatcataa
    21841 ataatgccat ccagttcttt ctcaatttta cgcaccgccg acattgtagg cgggaaagtt
    21901 tttggaagag tagtatgata atcttttttc ataatggttt tcattctctg gtaatgatct
    21961 ggggtaagtt gaaagaaagt acttactgtg acttgattgg aatttatcgc atcgagcact
    22021 aatcttgcaa caaaagattg attttgtttt atcagattga ttgcatcctg caatttttgt
    22081 tcgaaattgt tttcatcaaa agcctggttt ttttcaaaaa aattgaattg agacattcct
    22141 tttctcctat tatccagtat taatgattac ttccatgcaa tttcatagta acctttcttc
    22201 aattaatgaa caata
//
LOCUS       DBNCWK010000039        22170 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.039, whole genome shotgun sequence.
ACCESSION   DBNCWK010000039 DBNCWK010000000
VERSION     DBNCWK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22170
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.039"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..995)
                     /locus_tag="AC06IO_001343"
     CDS             complement(<1..995)
                     /locus_tag="AC06IO_001343"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIO9874543.1"
                     /translation="MAKDTKMINLALQGGGAHGALAWGIIDRLLEDGRINFDAISATS
                     AGAMNAAVLAYGFATGGKEGAREALYQFWKMVSDAGQIYNPLKKTPLEELLGIGIENS
                     MSFFIFDLMTKILSPYQFNPLNFNPLKEILEKIVDFEKIKSCKSIKIFISATNVRTGK
                     INVFDNKKISADAVMASACLPFMFQAVNIGEDYFWDGGYMGNPAIFPLIYNSECRDIL
                     ILHINPIYREHVPDTAAEILNRVNEISFNSSLMREMRAIAFVTKLIDSGWIKDEYKNN
                     LRKLYLHAIRADVSMESASVASKLNPEWSFISHLYEEGRKKGEEWLKSNFKHLGKV"
     gene            1332..1586
                     /locus_tag="AC06IO_001344"
     CDS             1332..1586
                     /locus_tag="AC06IO_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HIO9874544.1"
                     /translation="MNNISKITMAAVMAAGLSMATIPANADTPNDMEKCQGVAKAGKN
                     DCGTAQHACAGQSKTDGSTTDWIYLPKGTCEKLVNGTVKS"
     gene            1592..2461
                     /locus_tag="AC06IO_001345"
     CDS             1592..2461
                     /locus_tag="AC06IO_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HIO9874545.1"
                     /translation="MYSNHSINQAAPYSGCSGIGLRLEHIDDILKEQPTVDYFEVLAN
                     NYMKQSGVQFKQLLKIAEFYPMSLHSVGLSIATSAEPDYQYLRQIKDLAHCLNSKLIS
                     DHLCWTHANQFFTHELIPFPYTEETLSFIIEKTNRVQEYLNQPIMYENVSRYVTYRQN
                     TLSEAEFLNELSTATGCGILLDINNLYVNWYNHGDDPDKYLHSMNTKNVWQMHLGGFS
                     KQEGYLLDSHSDKVYQDVWQLYEKAQNLFINTPTVIEWDNDLPDFSLLYDEMCKAKTI
                     IKRVAERSINHYA"
     gene            2454..3233
                     /locus_tag="AC06IO_001346"
     CDS             2454..3233
                     /locus_tag="AC06IO_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HIO9874546.1"
                     /translation="MRNTLLETIQKEWIAQFKEEALSSPLKNRLARSNFDGISVYMES
                     HHATLFNHLKSTFPLCMRLVGENFWYALLHRYINNCESTQYDINQYGDSLPQFIANFE
                     PAKELPYLQQLCAIEWHWHTLKLLSPVKPFDFESFSGIDEISFQSLCFHLTPNLKILC
                     SEYPIYQIWAMNYFQQDKQIQISGCPEKVVIYQHNDLPCIMLISEEEYIFLELLSSGN
                     QTPWQKVATELSKWISEEKIGDTLTKALRYQWINHFSLEAI"
     gene            3230..3694
                     /locus_tag="AC06IO_001347"
     CDS             3230..3694
                     /locus_tag="AC06IO_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIO9874547.1"
                     /translation="MKALIAKTYLLADKIFDFLAPIFILLTRLYLAQVFFLSGLTKIS
                     NWQATLSLFQNEYMVPVMSPTFAAYLGTACELGFPVLITLGLGSRLVLFGLFLFNLFA
                     VFSYKFLWTPDGAVGLTDHMQWGIMILMLLCTGPGKLSIDYLIRRFYLKLDN"
     gene            3909..4562
                     /gene="rpe"
                     /locus_tag="AC06IO_001348"
     CDS             3909..4562
                     /gene="rpe"
                     /locus_tag="AC06IO_001348"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIO9874548.1"
                     /translation="MTFQILPSLLSADMTRLGEEVDAVMKAGADFIHFDVMDNHYVPN
                     LTFGPAFCDALIKKFPNLPIDVHLMTTPVDTLIEAFAKAGAKRISIHPDATIHLDRSL
                     QLINNLGCEAGLVLNPATPIDTLTWCAHNLKFVLVMTVNPGFGGQKLIPEVIPKLSKI
                     RQQHPNLDLCVDGGVTPQNIASLAHAGANQFVAGSAIFNSSNYSETIKRMREQLASI"
     gene            4604..6385
                     /locus_tag="AC06IO_001349"
     CDS             4604..6385
                     /locus_tag="AC06IO_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIO9874549.1"
                     /translation="MKRLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEHLPINPS
                     PEFLEFVKESTPLANKLRDKWLYELARIKDWANYNKYYQPTNDINLRCFAQIAKFNLG
                     LYKEAIAGSIPIWLTGESRPQACNTLFALLLKQDNFDQKFITQRITLALDKRNVLLAR
                     YLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKL
                     WQEKKTNIMLNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFA
                     LKNKNWKQVEKLINNSPNKEMPCWQYWLARSLEEQGKKAESIKIYEPLAKNRHYYGFL
                     ASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQVKNFYNSKQLLQASRLLNDFISELPK
                     EEASALVYWIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKVYASKYAIPPEL
                     VYAIIRQESGFREDVTSSAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINI
                     GIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQETRNYL
                     KNVMAFYIVYQYRLNQQPDMNRFLTPL"
     gene            complement(6686..8155)
                     /locus_tag="AC06IO_001350"
     CDS             complement(6686..8155)
                     /locus_tag="AC06IO_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIO9874550.1"
                     /translation="MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSTVEKKIS
                     SSAPVHLTWWKAFHDPLLDRYISEAATNNLDIKAAEARIRQARTMKQISLANFYPQIN
                     GSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
                     KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYK
                     AGVSSEVDVSLAEGQLKNTESVLPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSL
                     PKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFFPQFNLSVSNLAFQS
                     TSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVAQQAAIQYKKIILQALQE
                     AQSALVRYSQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAET
                     QEVNSEVSSLTALISLYKALGGGWENFEASSSPKHPRIL"
     gene            complement(8164..9288)
                     /locus_tag="AC06IO_001351"
     CDS             complement(8164..9288)
                     /locus_tag="AC06IO_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9874551.1"
                     /translation="MAKLSPTTRIFALVIKEILTLFKDPKGRIVLIAPPLLQLLIFSF
                     AATMEIKNISIGILNQDSGKHGYEVIQRFIGSPTFSKVFFIQKQSDIKPIIDNQEAMV
                     VMTIPQDFSRKIESNSQANIQLILDGRRSNATQIVNGYISSIIENYNQEIQKTHGAIM
                     TPPVMVTRSWFNENLLYLWFTVPSLVCILSMLISLVVTALSVARERELGTFDQLLVSP
                     LTPYEILIGKTIPAIIIGLAEGLLMWVAAVWLFDVPFYGSPLLMVYILFIFIMSTVGV
                     GLFISSMCKTQQQAILGVFIYMVPVVTLSGYASPVENMPEWLQDLTWFNPLKFTLIAV
                     IGIFLKGMSALEVFITTWPLLLVGAVTLFIAGWFFKQRME"
     gene            complement(9288..10409)
                     /locus_tag="AC06IO_001352"
     CDS             complement(9288..10409)
                     /locus_tag="AC06IO_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9874552.1"
                     /translation="MNIRYLKALVIKETLQIIRDPSTILIAFVLPLILLFIFGYGVNL
                     DTDQVKFGLALESYDSSTVSLSQSFSSSRFLDVSISTNRQAFRYGLVNGTIRGFIDIP
                     QRFTADSLSGNPQPALQTIVDGSYPQVAGFAENYALGALQTWISSQAYEHGQMTLKSG
                     INLEPRYWYNQELKSRNFLIPGSIVIVMTLIGTLLTSLVIAREWERGTMEAMMATPIS
                     IVDIILGKLIPYFVLGMGSMFLCTIIATWYYGVPFQGTLLALTLVSAVFLVAALGQGL
                     LISTLARDQFVASQLALMSAFLPSFILSGFIFEINSMPKWIQLLTHLFAARYFVTSLQ
                     TLFLTGNIWSLLIRCLFAIALIGFAFFFITAHKTRKRLD"
     gene            complement(10427..12169)
                     /locus_tag="AC06IO_001353"
     CDS             complement(10427..12169)
                     /locus_tag="AC06IO_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIO9874553.1"
                     /translation="MNSSKPLVSINGVSKCFPGNTDPALDNVSATIFEGQITGLVGPD
                     GAGKSTLMRLICSLMMPTQGTITVDSLNTRTDSEKIHAITGYMPQKFGLYEDLTIIEN
                     LRLYAELRNLHGEKREEQFRTLLKFTALEPFQKRLAGNLSGGMKQKLGLACALMGEPK
                     LLLLDEPSVGVDPISRRELWTMVQGLLGRGMGVVWSTAYLDEAEKCDQILLLNAGKPI
                     YHGKPGDFLQKTKDRTFQICGVSLLNRRQALMNALNTPEVIDGVIQGKNIRIVISDKK
                     IKPSLDGITREPGVHFQEVESRFEDAFIDSLKTKISGRSLLAEHIAEKPHSDKPIIEA
                     RKLTKRFGNFTAVSNNEFTIKRGEIFGLLGPNGAGKSTTFKMMCGLLQPTQGQAFVMG
                     LDLKTSSSEARSRIGYMAQKFSLYSHLDALQNMRFFSGLYGLSGKRQEAQINSMIEIF
                     DLKKHLKQNSGDLPLGFKQRLALACAVMHEPDVLFLDEPTSGVDPLTRREFWTHINGM
                     VNKGVTVMVTTHFMDEAEYCDRIALIYRGKNIATGTPDDLKDKVRNSTLPNPTLEDAF
                     IELIRLDELREPVH"
     gene            complement(12172..13017)
                     /locus_tag="AC06IO_001354"
     CDS             complement(12172..13017)
                     /locus_tag="AC06IO_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9874554.1"
                     /translation="MSKKAIAISIVIALFFITAIWFAYSFVKQEENQNWLTLYGNIDI
                     RDVSLSFRVSGRILSMDVDEGAHVKKGQVLARLDKDTFIANLETAKAQLEQAIANQKN
                     ASRTYKRREQLVDDGAVSKALFDDAVAMKDSSSAEVSVAKANLEKAQISLNDTEIHAP
                     THGTILTRIQEPGAIVNVAEPVYTLAIDNPVWVRTYIDEPFLGRIYPGQDALVYTDSN
                     PEKPYKGHIGFISPQAEFTPKIVETTQLRTDLVYRIRVVIDQPDNGLRQGMPVTVKIH
                     LKANS"
     gene            complement(13408..18615)
                     /locus_tag="AC06IO_001355"
     CDS             complement(13408..18615)
                     /locus_tag="AC06IO_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HIO9874555.1"
                     /translation="MKIPVQLKNLGGNIPENDIALQVSYFVKSQNEELLPQSDAYIAP
                     ASGKIDVSFGNNLARNKDVFFAVALQSNNILAQTSTSATQLRDEVPITLEFDYSPLVL
                     SEPDDQIVSLRPLFVYGRLLDKKGKKKLEDVQIIFEATRANDGGLQPIASVRTEADGY
                     FFIEYPQGSFIDAYARVGLPLRDNPIPVRLDEIRIDGDISNKVFPRNVILVAELMEDE
                     EATVEKECGCEVLDVHESKRILEEYSFYSVVRTTEPEILGYVLKEEDEITLDEVLTQH
                     PIRIWEIIDPVFQLPAFSHLANRARVNLPEVTERSINTEITTRAHGRTNTAPNPPQDN
                     ELAETLKQIKINRGVLNNFLQRETSITKDNIVNLIEMNESYRFKQKMTLGETKPLGRV
                     VLNSENSVDWDLEPTLYQAVSVAHGHLLHFKSEWIADGYSLGDLLYSLPLAPGQKKQI
                     VVFDWERRESATNIQSLEYEESLYNSLSRDRDIFEITKGVIEENIKGKSSATTASASA
                     GIGGVVGGLLFGVSGGVGHSGSTASQNSLRQTSASDLQKIRDRIVQSANAVRSQRSSV
                     IQTVSQGERFEVSSETVANYNHCHAITIQYYEVLRHFKVRQRFAEARECLFVPLLMSQ
                     FELNKLLRWRESLQFALLNPNLSKGFDAAERIKNKWVGSNFPGGTFASEKILNASGNF
                     QIKFIIRRPDDKMVEVDDYSKPIYGGQNMIIGYQKKTVEDINEANWQSLIPFLGSDTP
                     KGFYDHHLRDAKNKDEVFHRLLGEKIATAFVGALTFHVADETGNEISSISFDTTLTSK
                     YRKEGVLNVSVRFMGPSQFSRDKVHYIKIRCGTTNVLPDYSSIIVTSGFIRYKTPHYE
                     GFLCRFQSIYDDLSPSDGVTLYAGPSLDELRDPRKEDIALVNSLIDHLNDNLEWYHKA
                     IWQLMPAERRFLLLDGIVLPGNKGLGRSLASLVENELIGIVGNSLVFPVAKGLNLDPN
                     FGASDSLTDYYMVAAGDPVNVSIPTKGVYAEAMMGRCNSCEEKDESRFWRWEESPIPD
                     TPTTINPISTESRRAEPGNLQPTAFPNPMVNIQNAPNAPDPTGLAGTLSLLGKGDSFR
                     DITGLTQNQTNALEALKASFDATKTFGQEAAKLEIQKMMDKRLDNAIKAINNNPNLDP
                     KQKMELTEKALNAYLGAGANTTQPPKDANADKLQEGINKNLDKVNSSKSGEMSVTKPD
                     GTKVNTKFDGGTTSPAIETKIGKGGITPMKQENSNACWATVATMMLRWKDSKNYTIEE
                     VLTKAGSQYLNYYKQKIGLPYTEKQNFITSLGMKGEPLANYTAQNYRDWLVEYGPLWV
                     TTDTDQSEGFSAHALIITGISSDLKSLEVIDPLQGRKLVQSFDEFANAFKELITDSDL
                     LPTTQVVHFLEKIEATEGAPTPADIVKSLNEYNPSSSETLVINMVEFNTGISGITNYK
                     DWKTDSSVIHNKRKNAYRDPFGIKHLVLHETAAESGDGFDDSNNETSHMSVKRDETIL
                     QFNDLVEFENHGSGMNTTSIGIEFVNRGWLSSSTADGGEGIPAKESSLTAAQKETYKE
                     ANGYLWAFWGYGFNIYRVPPSIDQLEKEVELVKWLTRDFPALLQSISGISIYNLFPSI
                     DDTWLQLISYEEVKNIWTFKAADIPPEAERTEKNLFVMTTGYEYLEPSYLTDKSGIIS
                     HNAFYENHSDGSFLTLYTWLRLEKGKSKTDALDIAKKLMKDHFIRVSLTSDTDKKIIL
                     LNVKDGNLV"
     gene            18848..19540
                     /gene="cmpA"
                     /locus_tag="AC06IO_001356"
     CDS             18848..19540
                     /gene="cmpA"
                     /locus_tag="AC06IO_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-OmpA-like family protein CmpA"
                     /protein_id="HIO9874556.1"
                     /translation="MSRKLANTRILGYGLMICFLAGCFHPPYNNFQPDRRAVKRVGVD
                     TGIGAVAGVIASGTASGTLIGAAAGGTVGLVASIYRDSKRKIIRDLQKQDIQYVEYGD
                     TRTLIIPTDKYFMFSSPRLNEICYPGLNNVIRLLNFYPQSTIYVAGFTDNVGSRSHKR
                     KLSQAQAETMMTFLWANGIAAKRLKAEGYGDKNAISDNAIIHGSAQNRRIEIQWFTSP
                     AQPPQPQMAYVK"
     gene            19551..20741
                     /locus_tag="AC06IO_001357"
     CDS             19551..20741
                     /locus_tag="AC06IO_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9874557.1"
                     /translation="MRSRFIGGILLIVGTSIGGGMLALPVANAATGFWQSSLFLLICW
                     AFMTFGAFFILEANLYLSRGKHMVSMAKATLGDYGLLTAWISYLFLLYTLLSAYISGG
                     ADVLNSLLYKVNWHLSDWQASSLFTLIFGLVVYGGIHSVDVANRALMFGKLAVYCILV
                     ILIAPHIEFHYFQHGDYRQIGGAIMILITSFGFAIIVPNLRDYFDDDIKTLKKVIFIG
                     SLIPLICYIAWDAVIIGSLPSAGTSGLESLMQNPRTTSALASLLTDKVENTTISALFN
                     FFTSICMLTAFLGVSLCLFSFLADGLKLRERGRHGIGLFVLTFLPPLLIVIYYPGAYI
                     YALRYAGIFCIILLLLLPALMCYFGRKRYKSPFWVPGGKISQFAVIIISVVLLAHEFI
                     ALFK"
     gene            20942..22138
                     /locus_tag="AC06IO_001358"
     CDS             20942..22138
                     /locus_tag="AC06IO_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9874558.1"
                     /translation="MINSKLIGGILLIVGTSIGGGMLALPVSTAEVGFANSLFFLFFC
                     WLVMTAGALLILEVNLRLPAGSNMISMAKSTLGLPGQVIAWITYLLLLYTLLAAYISG
                     GSDVFSSLLHKAHIDLPNWITSVIFTAMFSLIVYSGIRSVDYVNRGLMFGKLGVYILL
                     VIIISPHVSTAGLTGGSAKAFTGTLMILVTSFGFASIVPSLRDYFNDDIRSLRRVILF
                     GSLIPLFCYIVWDAVIMGVISREGENGLLSLMNSDHATSGLTEALSQSVSSQWISGFF
                     GFFTSICMVTAFLGVSLGLFDFLADGLKLKKSGNQGKYTLALTFIPPLAVVLFNPGIY
                     LHALSYAGVCCVILLLLLPAIMAWKGRQTCSQSSSETHLIPGGNPALSLIGFIAIGLL
                     IIAIMY"
BASE COUNT         6643 a         4597 c         4124 g         6806 t
ORIGIN      
        1 acttttccaa gatgtttaaa attactcttc aaccattctt ctcccttttt gcgtccctct
       61 tcataaagat gactaataaa agaccactcg gggttaagct tactggctac tgaggcggat
      121 tccattgata catctgcgcg aatggcatgt aaataaagtt tcctgaggtt gtttttgtat
      181 tcatccttaa tccaaccact gtctatcaat ttagtaacaa aagcaatcgc ccgcatttcc
      241 cgcattaatg aagaattaaa actgatctca ttcactcgat ttagaatttc ggctgcagta
      301 tccgggacat gctctctata aataggattg atatgaagaa ttaaaatatc cctgcactcg
      361 gaattataaa tcagagggaa aatagctgga ttccccatat aaccaccatc ccaaaaataa
      421 tcctcgccaa tattcactgc ctgaaacatg aaaggcaggc aagcagaggc cataaccgca
      481 tcagcagaaa ttttcttatt atcaaataca ttgattttac ctgtgcgaac attagtagct
      541 gagataaata ttttaatgct tttacaggat ttaatttttt caaaatctac aattttctct
      601 aatatttcct taagtgggtt aaagtttaag ggattaaatt gatagggaga aagaattttg
      661 gtcatcaaat caaatataaa aaaggacatt gaattttcaa tccctatgcc caatagttct
      721 tccaaaggag tttttttaag tgggttatat atctgcccgg catcgctcac cattttccaa
      781 aattgataca gagcttctcg agccccctcc tttccaccgg ttgcaaatcc ataggcaagt
      841 acagcagcat tcatagcacc agcactcgtt gcagaaatgg catcaaaatt aatacgacca
      901 tcttcaagaa gtctgtcaat aataccccag gcaagagcac catgagcacc acctccttgt
      961 aaagcaagat taatcatttt ggtatccttt gccatgatac aatactcctc ttccttttgc
     1021 tgtgatttat taagtataag gcaaagatgc aagaaatgtt ttctatccca tttggaataa
     1081 aaacaaacca tgttaaatat tttccatctt tgagaaataa gttgatgtcc tgtcacaaat
     1141 cgaaaaatgg tactatacta actacgttat tttattagac ctcttgccta acctgattaa
     1201 ttttttagag caagaaacaa gcattttgag gagctgtgtt tgcatacagg aatataatga
     1261 aagagcccgg ttaggcaagg ggtttattaa ataacatatg gattaatcac atataacaaa
     1321 ggataggtat catgaataat atttctaaaa taacaatggc tgccgtgatg gctgctggac
     1381 tttcaatggc taccatacct gctaatgcag atactccaaa tgatatggaa aaatgccaag
     1441 gcgtggccaa agctggaaaa aatgattgtg gtacagctca acatgcctgt gctggccaat
     1501 ctaaaacaga tggttcaaca actgactgga tttatctgcc caaaggtact tgtgaaaagc
     1561 tggttaacgg cacagttaaa agctaattag aatgtattca aatcattcta tcaatcaagc
     1621 cgcgccttat tctggatgtt caggcattgg attgcggctt gaacacatcg acgatatttt
     1681 aaaggaacag cccacagttg attattttga agtcctggct aacaactaca tgaaacaatc
     1741 aggagttcaa tttaaacaat tgctcaaaat agcagaattt tatcccatga gtttacatag
     1801 tgttggcttg tctattgcca catctgctga acctgattat caatatttgc gacaaataaa
     1861 agatttggcc cattgtttaa actccaagct aatatcagat catttatgct ggacccatgc
     1921 caatcaattt tttacccatg aattgatacc atttccctat actgaagaaa cgctctcatt
     1981 cattattgaa aaaacaaacc gcgttcagga atacctaaac cagccaataa tgtacgagaa
     2041 cgtatcaagg tacgtcactt atcgacaaaa tacattatct gaagccgagt ttctcaatga
     2101 gttaagcact gctacaggtt gtggcatctt attggatatc aataatttat acgtgaattg
     2161 gtataatcat ggtgatgacc cggataagta tcttcattcg atgaatacca aaaacgtatg
     2221 gcaaatgcat cttgggggat tttcaaaaca agagggatat ttgctggata gtcatagtga
     2281 taaagtatac caagacgttt ggcaacttta tgaaaaggct caaaatctct ttataaacac
     2341 gccaacagtt atcgagtggg ataatgactt gccagacttt tcactcctgt atgacgaaat
     2401 gtgtaaagca aaaacgataa taaagcgtgt ggcagaaagg agtataaatc attatgcgta
     2461 atactctgct tgaaacaatt caaaaggaat ggatagcaca attcaaagag gaggcactat
     2521 caagcccctt aaaaaacagg ctcgctcgat ccaattttga tggtattagt gtctacatgg
     2581 aaagccatca tgctacttta tttaatcatt tgaaatcaac ttttcctcta tgtatgcgtt
     2641 tggttgggga aaacttctgg tacgctttat tgcatcgcta tatcaataat tgcgaatcca
     2701 ctcaatatga cattaatcag tacggagata gccttcctca atttattgcg aattttgaac
     2761 ctgctaaaga gctgccttac ttgcagcagt tatgcgctat tgaatggcac tggcacacct
     2821 taaagcttct ttcccctgtt aaaccttttg attttgagtc tttttcgggc atagatgaga
     2881 ttagctttca atctttatgt tttcatctga cgcccaattt aaaaattctc tgttctgaat
     2941 atcctattta ccaaatttgg gctatgaact attttcagca ggataaacaa atacaaatat
     3001 caggttgtcc tgaaaaagtt gttatttatc agcataatga tttaccttgt attatgctaa
     3061 ttagtgagga ggaatatatt tttctggaac tcttatcctc tggaaaccag acaccatggc
     3121 aaaaagtggc aacagaatta agtaaatgga ttagcgaaga aaaaattggc gacactctaa
     3181 ccaaggcgct tcgttatcaa tggattaatc atttttcact ggaggctata tgaaagcact
     3241 tatagcaaaa acctatctgc tggctgataa aatttttgat tttcttgccc caatttttat
     3301 tcttttaacc cgcctttatc ttgcccaggt tttttttcta tctggcttaa ccaaaatcag
     3361 taattggcag gctactcttt ctttattcca aaacgaatac atggttcccg ttatgtcgcc
     3421 tacttttgcc gcgtatctgg gaacagcttg tgaactgggc tttcctgtat tgattacatt
     3481 aggacttgga tccaggctcg ttttatttgg actttttcta tttaacttat ttgctgtttt
     3541 ctcttataaa tttctgtgga cgccagacgg tgccgttggc cttaccgatc acatgcaatg
     3601 gggaatcatg attttaatgc tcttatgtac agggccaggg aaattatcaa ttgattattt
     3661 aatcagacga ttttatttga aattagataa ttaattgaat ctttaaactc catagatcga
     3721 aacatcaagc attctatacc atcctctctc taacgaatca gagaggatgg aattttgtta
     3781 tcttgatttg cttcagttaa attcaggatt aaatcaataa aactcgatat gaatccaaat
     3841 cgtaatgctg gaatgtttac caaagtcagc ttattatatg taaaagcaaa ccgttgaatt
     3901 actaaatcat gacattccaa attttaccct ctttactgtc agcggacatg acccgtttag
     3961 gagaggaagt agatgccgtt atgaaagccg gcgctgattt tattcatttt gatgtcatgg
     4021 ataaccatta tgtacccaac ctgacttttg gccccgcttt ctgtgacgct ttaatcaaaa
     4081 aatttccaaa tttgccgatt gatgtccatt tgatgactac cccggttgat actctaatcg
     4141 aagcatttgc caaagcaggt gcgaaacgta tcagtatcca tccagatgct actattcatt
     4201 tagacaggag tttgcagtta atcaataacc ttggatgtga agcgggttta gtgctcaacc
     4261 cagctacgcc aatcgacaca ttaacctggt gtgcccataa tctaaaattt gttttggtca
     4321 tgacagttaa cccaggattt ggcggtcaaa aacttatccc ggaggttata cccaagctct
     4381 ctaaaattcg gcaacagcac ccaaatctcg atctatgtgt tgatggtgga gtcacccccc
     4441 agaatattgc ttcattagca catgccggag ctaaccaatt tgttgcaggt tctgctatat
     4501 tcaatagcag taattatagt gaaaccatta aacgtatgcg tgaacaatta gcaagcattt
     4561 aaaagtgcca aataattgcc ctcaagcctt attaagagga attatgaaaa gattattaat
     4621 ttttttatgt gggcttctct gttctttcaa cttgtttgct ttatctggac aagcctacat
     4681 ggacagattt aatcgctatc tcgcatggta tgaacacctg cccattaacc cttcaccaga
     4741 atttctggaa tttgtaaaag aaagcacgcc gcttgctaat aaattacgag ataaatggct
     4801 atatgaactg gccaggataa aggattgggc aaattataat aagtattatc aacccacaaa
     4861 cgacataaac ctcagatgct ttgcgcaaat tgccaaattt aatttaggat tatacaagga
     4921 ggctattgca ggctctattc caatctggtt aacaggagag tccagaccac aagcatgcaa
     4981 taccctattt gctttattgt taaaacagga caattttgat cagaaattca ttacgcaacg
     5041 cataacactt gcactagaca aacgtaatgt tctcctcgct cgttacctgt taaaacaata
     5101 caaaacacct cacgatacag aaataaaaaa cttaaccagt gtttatgaga atccttccaa
     5161 tatttccaag ttgaaccctg gcaaattgaa cgattatttc tatctttatg gattaaaaag
     5221 attagtatcc attaacatgg ataaagcagt gaaattatgg caagagaaaa aaaccaacat
     5281 catgttaaat gagcggcaaa aacaagattt tcttgcacat ttggcattat acaaggccat
     5341 gagaaatcat gccgatgctc aggaatggtt tgccaaaata aaacctgaat actacaccga
     5401 tgtgttagtc gactggcaaa tccgctttgc tctgaaaaat aaaaactgga aacaagttga
     5461 gaaattaatt aacaactcac caaataaaga gatgccttgt tggcaatatt ggttggctcg
     5521 ctccctggaa gaacaaggga aaaaagcaga gtcgattaaa atttatgagc cactggctaa
     5581 aaacagacat tattatggtt ttctagccag caaacgcctg aaaaaaaggc ccagctttga
     5641 caatgaaaaa ccaacaacca acacagagat tctcaaacct tatcaaccgg tcatagagca
     5701 agttaaaaat ttttacaact ccaaacagct actgcaggca tcaaggttat taaatgactt
     5761 cattagtgaa ttacccaaag aagaagcaag tgctctggtc tattggatag caaccgaact
     5821 aaagtggcac ggaaaatcag tttacttaag caataatgac acactcaata atcaattagc
     5881 attaagattt ccattggcct acaaagacac aataaaagta tatgcgtcaa aatacgccat
     5941 accgcctgag cttgtatacg ctattattcg tcaggaaagt ggttttagag aagatgtaac
     6001 atcctctgct ggggctaaag ggctaatgca agtcatgcca caaacagcca gcgctgtttc
     6061 caaaaccagc aaaataccgt attcggatca taagcaattg tttttatctc aaaagaatat
     6121 caatataggg attgcttatt tacagcactt aaccaagcgc ttcagcaacc acccggttct
     6181 tgttgctgcc gcttacaacg ccggtcccag acaagtagtc tattggctta aaaatcaccc
     6241 gcccaaagaa atagatatct ggattgaaac acttccctgg caagaaacac gaaattatct
     6301 aaaaaatgta atggcctttt atattgttta tcagtaccgt ttaaaccaac agcctgatat
     6361 gaataggttt ctaacacctt tgtaagagat attttcatgg ttgtaaacgt aaatcgctct
     6421 atagccagat ctacgtttgc gagctacctg tctgactttt gctaatttaa cctaatgggc
     6481 gagagttata taagaagttg ttgatatccg gggccgtttg ggcttaggag atgagtaaac
     6541 atcgtctcga agccttggca caaaacatta aattctatac aaggcttcga gactgcataa
     6601 atgcctcctc agcccgaacg attcaagata atgacctacc ttatccatag cttattacat
     6661 taatttattg ataaaactcc tgattttaga gtatgcgcgg atgtttaggg ctacttgaag
     6721 cctcaaagtt ttcccaacct ccaccaagcg ctttatatag actaatcaac gcagtcaatg
     6781 aagaaacctc cgaattaact tcttgagtct cggcgttcag aagagcagcc tctgactgca
     6841 gtagtgataa ataatcatcc tcaccgtatt gaaatcgttt cttggcaaat tgcacagctc
     6901 tcttgttagc aataatggca gcgcgcaagc tatggtttgt atcgagcgat tggctatagc
     6961 ggacgagagc actttgagcc tcttgcaatg cctggaggat tatttttttg tattgtattg
     7021 ctgcttgctg agcaaccgcc ttcttactct caacattagc tctaatctgg ccaccatgaa
     7081 atatgggtaa actgacttga ggacccatga gccaaaatcc actgctggat tgaaataacg
     7141 tatccacaga agtgctctga aaagcaagat tggaaacaga aagattaaat tgcgggaaaa
     7201 agtcagcaac tgccacacca atttcagcta cggcagacgc caattgcctc tctgttgccc
     7261 ttatgtctgg acgctggcgc agaatatctg aacgtaatcc cataggtact ttttgcggtg
     7321 gtttgggtaa agattgaatt tttgcaatat ctttgagaat tgtttgcgga tctttaccca
     7381 gcataacggc aatttgataa gcatttacct gtatttgggt aatataattg ggtaaaacag
     7441 attctgtatt ttttaactgg ccttcggcca aagaaacatc gacttcgctg ctaacacctg
     7501 ctttatagcg ttggcggaca agagccaacg tttttctttg caattccaca tttttttgcg
     7561 caataccata caactgttgt gcgcctctca attctatata acttctagcg atctcggcta
     7621 tcgtagatag taaaatggta cggcgtgatt cataagcctc ttccaggctt gctttgcttg
     7681 cttcaacttt tcttcttgtt ttaccaaata aatccaactc ccattgtgca tcaaaaccca
     7741 aattatatat ccattgattt ctatcaatta gagtatatgt tggagaattt gcaaaaatac
     7801 cctccaacgt acccttactg atttgcaagg aattagcact tgctgatcca ttgatctgcg
     7861 gataaaaatt ggcaagggaa atttgtttca ttgtgcgggc ttggcggata cgagcttctg
     7921 ctgctttgat atcaaggtta ttagtagctg cctcgctaat gtatcgatca agcaatggat
     7981 catgaaatgc cttccaccag gttaaatgga caggggcact actggaaatt tttttctcta
     8041 ctgtgcttgt tggccatttc tcctgaattt taatgtctgg gcgttgataa tcaggaccga
     8101 cagtacagcc aaataaacaa gaaatactga agagtagcgc taattttctg tccatactgt
     8161 ttcttactcc atacgctgct taaaaaacca gccagcaata aataatgtaa cagcccctac
     8221 caatagaagt ggccatgttg tgataaaaac ttctaaagcc gacatacctt ttaaaaaaat
     8281 gccaattact gctattaaag taaattttaa tgggttaaac cacgttaaat cctggagcca
     8341 ttccggcatg ttttcaaccg gagaagcgta accggaaagc gtcacaacag gcaccatgta
     8401 gataaatacc cccaaaatag cttgctgctg ggttttacac atagaagaaa taaataagcc
     8461 aacacccacc gttgacataa taaaaataaa caaaatataa accatcagta acggagaacc
     8521 ataaaaagga acatcaaata gccaaaccgc agcaacccac atcaagagac cttccgccaa
     8581 accaatgata atggcaggaa tagtcttgcc aataagaatt tcatagggag taagaggtga
     8641 tactaataat tgatcaaagg ttcccagctc tctttctctg gctaccgata aagcagtaac
     8701 cacaagagaa atcagcatgg ataagatgca taccaatgaa ggaacggtaa accaaagata
     8761 aagcaaattt tcgttaaacc aacttcgagt gaccatcacg ggaggcgtca tgattgcacc
     8821 atgcgttttt tgaatttcct gattatagtt ttcaataata ctactgatat aaccatttac
     8881 aatttgggtt gcattagaac gtcgaccatc caaaattaac tgaatattcg cttgtgaatt
     8941 tgattcgatt ttacgagaaa aatcttgtgg gatggtcatt acaaccattg cttcctggtt
     9001 atcaatgata ggtttgatat cagattgctt ttgaataaaa aaaacttttg aaaaagttgg
     9061 tgagcctata aagcgttgaa tcacttcata accatgcttt ccggaatcct ggttcaaaat
     9121 gcctatagaa atatttttaa tttccatagt agctgcaaag gaaaaaatca ataactggag
     9181 gagtggagga gctatcaata caattcgtcc cttgggatct ttgaataaag tcaatatttc
     9241 tttaatgaca agagcaaaaa ttcttgttgt tggagataat tttgccatta atcaagcctt
     9301 tttctggttt tatgcgcagt aataaaaaaa aatgcaaatc caatcagtgc aatcgcaaaa
     9361 aggcaacgaa tcaaaagaga ccaaatgtta cccgttaaaa acaaggtttg cagacttgtc
     9421 acaaaataac gtgcagcaaa caaatgagtg agtagttgta tccactttgg catcgaattg
     9481 atttcaaaaa taaacccgga aagaataaat gaaggcaaaa acgcagacat taaagccaat
     9541 tgcgaagcca caaactggtc tcttgcaaga gtagatatta ataatccctg ccctaaggca
     9601 gcaaccagaa agacagctga aactaatgtc agtgccaaaa gagtaccttg gaaaggtact
     9661 ccataatacc atgttgcaat aatagtgcat aaaaacatgg aacccatccc taacacaaaa
     9721 tagggtatta attttcccaa gatgatgtca acaatagaaa ttggcgttgc catcatcgcc
     9781 tccatcgtac ctctttccca ctcacgagcg atcacgagcg aggtgagtaa tgttccaatc
     9841 aaagtcatga caataactat tgagccagga ataagaaaat ttctgctttt tagctcctgg
     9901 ttgtaccagt atctgggttc aaggttaatt ccggacttaa gtgtcatttg cccatgctca
     9961 taagcttggg aactaatcca ggtttgcaaa gccccaagcg cataattttc tgcaaaaccg
    10021 gcaacttgcg gataagaacc atcaactatg gtttgcagag ccggctgggg atttccactc
    10081 aggctatcag cagtgaaacg ttgaggaata tcaataaaac cacgaattgt tccattaacc
    10141 aagccataac gaaacgcttg tctgtttgtt gaaatagata catccaaaaa tcgggaactg
    10201 ctaaaagatt gagaaagact gacagtcgac gaatcataac tctctaaagc cagaccaaac
    10261 ttgacttgat cagtatccag attaacacca taaccgaaaa taaaaagcaa aattaaaggc
    10321 aatacaaagg caattaatat agtactggga tcacggataa tttgcagagt ttccttaatg
    10381 accaaagcct ttagatagcg aatattcaac cactattctc cctgtgttaa tgtaccggct
    10441 cacgcaactc atccaatcgg attaactcga taaacgcgtc ttctaaagtg ggattgggta
    10501 atgtggagtt acgtacttta tcctttaagt catcaggtgt tcctgtggca atgtttttcc
    10561 ccctgtaaat aagagcgata cgatcgcagt attccgcttc atccataaaa tgagtggtta
    10621 ccatcacggt cacaccttta ttcaccatac cattaatatg tgtccaaaac tcacgtcggg
    10681 tcaatggatc aacccccgaa gtcggctcat caagaaataa cacatcaggc tcatgcatta
    10741 cggcacaagc cagagcgaga cgttgtttaa atcctaatgg aagatcaccc gaattttgct
    10801 ttaagtgctt tttaagatca aaaatttcaa tcatgctgtt gatctgtgct tcctgccttt
    10861 ttccagataa cccatagaga ccagaaaaaa agcgcatgtt ctgcaaggca tccaaatgac
    10921 tgtatagtga aaatttctga gccatatacc caatgcggct tcttgcttca gaggatgaag
    10981 tctttagatc gagtcccatt acaaatgctt gtccttgtgt gggttgcaat aaaccacaca
    11041 tcattttaaa tgtggttgat ttacctgctc cattagggcc taataaacca aagatttcac
    11101 cgcgtttgat tgtaaattca ttattcgaaa cggcagtaaa attaccaaaa cgtttcgtta
    11161 actttcgtgc ctcgatgata ggcttgtcac tatgaggctt ttcagcaata tgctcggcta
    11221 acaaagagcg accacttatt ttagttttca ggctgtcaat aaaagcgtct tcaaaacgcg
    11281 attcaacttc ttgaaaatga actccgggtt cccgagttat accatccaaa cttggcttta
    11341 tttttttatc actaatgaca atacgaatat tcttaccctg gatgacacca tcaatgactt
    11401 caggggtatt aagggcattc atcagagcct ggcgacgatt tagcagggaa acaccgcaaa
    11461 tttgaaaagt ccggtctttg gttttttgca aaaaatctcc cggttttcca tggtatattg
    11521 gtttgccggc attgagtaaa agaatttggt cacacttttc agcttcatca agataagcag
    11581 ttgaccaaac aacgcccata ccccttccaa gcagcccttg gaccatagtc cacaactccc
    11641 ttctggatat tgggtcaacg ccaacactcg gctcatcgag caataacagc ttaggctccc
    11701 ccattaaggc acaagccaac cccaattttt gcttcattcc ccctgagaga ttaccagcca
    11761 gacgtttctg aaaaggctcc agtgctgtaa atttaagtaa tgttctaaat tgctcttcac
    11821 gtttctcgcc atgcagattt ctaagttcag catagagcct caaattttct ataatggtta
    11881 aatcttcata caaaccaaat ttttgtggca tatagccagt aatcgcatga attttttccg
    11941 agtcagtccg ggtattgaga ctatctacag taattgttcc ttgcgtaggc atcatcaggc
    12001 tgcatataag acgcataaga gttgatttcc cagccccatc cggtccaacc aaacccgtga
    12061 tttgcccttc aaaaattgtt gcagaaacat tatctaatgc cggatcagtg tttccaggaa
    12121 aacacttgct gactccatta atactgacta gtggttttga actattcatt attatgaatt
    12181 cgcttttaaa tggattttca cagtaacagg cattccttgc cttaatccat tatccggttg
    12241 atcaataacg actcttatac gatagacaag atccgttctc aattgagtgg tttcaacaat
    12301 ttttggtgtg aattctgctt gtggggaaat aaagccaata tgacctttgt agggcttttc
    12361 cggattggaa tccgtgtaaa ccaaagcgtc ctgtccagga taaattctac ctaaaaaagg
    12421 ttcatcaata taagtcctta cccataccgg attatcaata gccagggtat aaactggttc
    12481 cgcgacatta acaatagccc ctggctcctg aattctggtc agtatcgtcc cgtgagtcgg
    12541 ggcatgaatt tccgtatcgt tcagtgatat ttgagctttc tcaagattcg ctttagccac
    12601 agaaacttcg gcactactgg agtctttcat cgcaacggca tcatcaaaaa gcgcttttga
    12661 aacagccccg tcatcaacta attgctcacg tcttttataa gttctgcttg cgtttttttg
    12721 attggcaatg gcttgttcca attgggcttt cgccgtttcc agattagcta taaaagtatc
    12781 tttatcaagc ctggcgagta cttgcccctt cttaacatga gcaccttcat caacgtccat
    12841 agacaggata cgccccgaga cacgaaagga taatgaaacg tcacgtatat caatatttcc
    12901 ataaagagta agccaatttt gattttcttc ctgtttaaca aagctgtacg caaaccaaat
    12961 tgctgtgatg aaaaaaagtg cgatgacgat acttattgct atcgcttttt tactcatata
    13021 gttctccatg aattccttgg cgtactaacc ctttgttcgc attatatttt cttcaggact
    13081 tataaaactc caatctcttt gttaaaaatg ttttcaatgc ggtcattgag tctatctaaa
    13141 cgccctcatt acaaccattt tttgcctggg ccttggtttt tttcgtaagt tctattctta
    13201 tcaataaagc ataaatagaa agtcataatg ccataattta ttattttaaa atagttttaa
    13261 atcgggaaca tagagcaagt tgatactgat taataatcag agccacggcc gttagggaat
    13321 ggagccaaat tcatacagac actctaccca taaaaggtgt atctcaaata tatatagagt
    13381 tgcattacct gattctggat gcccatatca aaccaaatta ccgtccttga cattcagcaa
    13441 aattattttc ttgtctgtat cactcgttaa agaaacacga atgaaatgat ccttcatcag
    13501 tttcttggca atatccaacg catctgtttt tgattttcct ttctccaaac gtagccaggt
    13561 atataatgtc aagaaagaac cgtcactgtg attctcgtaa aaagcattat gtgaaataat
    13621 tccactctta tctgttaagt aactgggttc cagatactca tatcctgttg tcatgacaaa
    13681 gagattcttc tcagttcttt ctgcctcagg gggtatgtct gctgctttga aagtccatat
    13741 attctttact tcctcgtagg agataagttg tagccaagta tcgtcaattg agggaaagag
    13801 attataaata gaaataccgc tgatggattg caataaggca ggaaaatctc tggtgagcca
    13861 cttcacaagt tccacctctt tttctaactg atcaatagac ggaggaacac gataaatgtt
    13921 aaagccatat ccccaaaatg cccataaata cccattagcc tctttgtagg tttctttttg
    13981 tgctgcagtc agactggatt ctttggcagg tatcccttct ccaccatccg cagtggaaga
    14041 agatagccat cctcgattca caaactctat gccaatactg gttgtgttca tccccgagcc
    14101 atgattttca aactcgacca agtcattaaa ttgtagaatg gtttcatctc ttttaactga
    14161 catatgcgag gtttcattat tggaatcatc aaaaccatca ccgctttcag ccgctgtttc
    14221 gtggagcaca aggtgcttaa taccaaaagg atctctgtaa gcatttttac gcttgttgtg
    14281 tatcacggaa ctgtctgttt tccaatcttt gtaattggtg atccctgaaa tacctgtatt
    14341 gaactcaacc atattaatta ccaaggtttc tgagctggag ggattgtatt catttaaact
    14401 cttaacaata tcagcaggag ttggcgctcc ttctgttgcc tcgattttct ccaggaaatg
    14461 aaccacttgg gttgttggta aaagatcact gtccgtaata agctccttaa atgcattggc
    14521 aaattcatcg aacgattgaa caagctttct gccttgtaag ggatcgataa cttccaggga
    14581 ttttaagtca ctactaatac cagtaataat caacgcatgt gctgaaaaac cttcgctttg
    14641 atcagtatct gttgtgaccc ataacggccc atactccact aaccaatcac ggtaattttg
    14701 tgcagtataa ttggctaagg gctccccttt catgcctaaa gaagtaataa aattctgttt
    14761 ttctgtatag ggcaagccaa tcttttgttt gtaatagttt agatattgag atccagcttt
    14821 cgttaaaacc tcctcaattg tataattctt gctgtctttc catcgcaaca tcatggtagc
    14881 cactgttgcc caacaggcat tgctgttttc ttgcttcata ggagtaatgc cgcctttacc
    14941 tattttggtt tcaatagctg gagacgtggt gcccccgtca aattttgtat tgactttagt
    15001 cccatctggt tttgttacag acatctcgcc actcttgctg ctgttcactt tgtccaggtt
    15061 tttattaatg ccttcctgca acttgtcggc attggcatct ttcggcggtt gcgttgtatt
    15121 agcccctgcg ccaagataag catttaatgc cttttctgtg agctccattt tttgttttgg
    15181 atccagattt ggattgttat taatcgcttt aatcgcattg tctaatcgtt tatccatcat
    15241 tttttgaatt tccaatttcg ccgcctcttg cccaaatgtt ttggtcgcat caaaggatgc
    15301 tttcaaggcc tccaacgcat tggtttgatt ttgagtaagt cccgtaatat ctctgaatga
    15361 atctcctttt cccaataaag aaagtgttcc cgccaaacca gtgggatcag gggcattcgg
    15421 tgcattttga atattaacca tgggattggg aaaagcagtc ggttgcaagt taccgggttc
    15481 cgctcttcga ctctcggtac tgattggatt gattgtagtt ggagtatcag gtataggaga
    15541 ttcttcccaa cgccagaagc ggctttcatc tttttcttca caagaattgc aacgtcccat
    15601 catcgcctcg gcatacacac ctttcgttgg gattgaaaca ttgacaggat caccagcagc
    15661 caccatatag taatcagtca gagaatccga agcgccaaaa ttaggatcca gatttaatcc
    15721 tttcgccaca ggaaaaacca ggctattacc tacaatacca attagctcat tttccaccag
    15781 agatgccagg cttctgccta aacctttatt accgggtaat acgataccat ccaggagtaa
    15841 aaatcttctt tcggcaggca tcagttgcca gatcgctttg tgataccact ccaaattatc
    15901 gttcaaatga tcaatcagcg aattcaccag ggcaatatct tcttttcttg gatcacgtaa
    15961 ctcatcaagt gagggaccag catatagcgt tacaccatcg ctaggagata aatcgtcata
    16021 aatactttgg aaacgacata aaaagccttc atagtgaggc gtcttgtatc ttataaatcc
    16081 tgaagtgacg ataattgacg aataatcggg taacacattg gtcgtaccac aacgaatttt
    16141 aatataatgc actttgtctc tgctaaattg tgaaggtccc ataaaccgca cagaaacatt
    16201 taaaacccct tcttttctat attttgaagt cagagtcgta tcaaaggaga ttgaggaaat
    16261 ttcattacct gtctcatccg caacatgaaa cgtgagagca ccaacaaatg ccgttgcaat
    16321 tttctctcca agcaatcgat ggaatacttc atccttgttt ttcgcatctc ttaaatgatg
    16381 atcataaaaa cctttcggag tatctgatcc caaaaaagga attaaacttt gccaattcgc
    16441 ttcgttgata tcctcgactg tttttttctg atatccgata atcatattct ggccaccata
    16501 gataggcttt gaataatcat ccacttcaac catcttgtcg tcgggtctgc ggataataaa
    16561 tttgatttgg aagttacctg atgcattaag aattttctcg gaagcgaaag ttcctcctgg
    16621 gaaattgcta ccgacccatt tattcttaat tctttctgca gcatcaaacc cttttgataa
    16681 attgggattc aataaggcaa attgaaggct ttctcgccaa cgtaataatt tatttaactc
    16741 aaattgactc ataagcagtg gaacaaacaa acattcgcga gcttccgcaa aacgctgacg
    16801 cactttaaaa tgacgtaata cctcatagta ctgaatcgta atggcatggc aatgattata
    16861 gttagccact gtttcagatg acacttcaaa acgctctccc tgagaaaccg tttgaattac
    16921 tgaagaacgc tggctacgaa cagcattagc cgattgaaca atgcgatctc taattttttg
    16981 caagtcactt gccgaagttt gccttagact attttgagag gcggtcgagc cagaatgacc
    17041 aaccccgcct gagaccccga aaagcaaacc acccactact cctccaatac cggcgctggc
    17101 acttgcggta gttgcgctcg atttgccttt tatgttttct tcaatcactc ctttcgtgat
    17161 ttcaaagata tctctatcac ggctaagtga gttgtataag ctttcttcat actctaaaga
    17221 ttgtatattg gtagctgact ctcttcgttc ccaatcaaat actacaatct gtttcttctg
    17281 gcctggtgcc aatggtaagc tataaagcag atctccaaga gaataaccat ctgcaatcca
    17341 ctctgactta aaatgcagta aatgtccatg agcaaccgat acggcctgat atagagtagg
    17401 ttctaaatcc caatccacgg aattctcgga attcaatacg actcttccca gaggttttgt
    17461 ttctcccaaa gtcatttttt gcttaaagcg ataactttca ttcatttcaa tgagattaac
    17521 aatgttatct ttggtgatac tggtttctct ttgtaaaaaa ttatttaaaa caccgcgatt
    17581 aattttaatc tgttttaaag tttcagccag ttcattgtct tggggcggat taggtgctgt
    17641 atttgtgcgg ccatgtgctc ttgttgttat ttccgtattg atgcttcttt ctgtgacttc
    17701 tggcaggttt actcttgcac gatttgcaag atgactaaat gcaggtaatt gaaaaacagg
    17761 atcgataatc tcccaaatac ggataggatg ctgggttaat acttcatcca aagtaatctc
    17821 atcttcttct tttaagacat aacctaatat ttcaggttca gtggtgcgca ccacactata
    17881 aaaggaatat tcttccaaaa tccttttact ctcatgaaca tccagtacct cgcagccaca
    17941 ttccttctcg acagttgctt cttcatcttc cattaattct gcaactaaaa taacattgcg
    18001 aggaaatacc ttattcgaaa tgtcgccatc aatcctgatt tcatcaagcc ggacagggat
    18061 tggattgtca cgaagaggca aacccactcg ggcataggca tcaataaaac taccttgcgg
    18121 atactcgata aaaaaataac catctgcttc tgttcttacg cttgcgatgg gctgtaaccc
    18181 tccatcatta gcacgagtag cctcaaaaat gatttgaaca tcttcgagtt ttttcttgcc
    18241 ctttttatca agcaagcgcc cataaacaaa taatggtctg agagaaacaa tttgatcatc
    18301 aggctctgat aacaccagcg ggctataatc aaattccaaa gtaatgggta cttcgtctct
    18361 taattgagta gcggatgttg aggtttgagc cagaatattg ttgctttgta atgcaacagc
    18421 aaagaaaaca tctttatttc gcgcaagatt attcccaaag ctgacatcaa tcttgcctga
    18481 agcaggagca atataagcgt cactttgggg aagcaattcc tcattctgag atttcacaaa
    18541 ataagaaact tgcaaagcaa tatcgttttc tgggatatta ccacctagat ttttgagttg
    18601 gactggaatt ttcatagctt tttcggcaaa ccgggtaatg agccaggcat accgctcctt
    18661 gcattttggt gagttagcac attgaatttc ctttcacagt gcatcactct gtttcttcct
    18721 tgataatttc aattatagac gaagaaaatt atttttttaa atgcgtcctg taaaaatata
    18781 aagtgttaga ttgcgaaatt taatgagatg cttaataatg attaaatcat tgattaaagg
    18841 agtggatatg tcacgcaaat tagccaatac acgaatcctt ggttatggat taatgatttg
    18901 ctttcttgcg ggctgctttc atccccctta taacaatttt caacccgata gacgagctgt
    18961 taaaagagtg ggagtagaca ccgggattgg cgcagtagct ggagtaatag ccagtggtac
    19021 tgcttcagga actcttattg gggcagcggc tggtggtacc gtaggtctgg ttgcgagtat
    19081 ctaccgtgac agcaaacgca aaatcatcag agatctgcaa aagcaggata ttcagtatgt
    19141 tgaatatgga gataccagaa cattaattat tcctacagac aaatatttca tgtttagcag
    19201 ccctcgcctt aatgaaatat gctatcctgg cctaaacaat gtcatcagac tgttaaattt
    19261 ttatcctcaa agtacgattt atgttgccgg atttactgat aatgtgggat cacgcagcca
    19321 taagagaaaa ctttctcaag cgcaagctga aacaatgatg acttttctct gggctaatgg
    19381 tattgcagcc aaaagactaa aagcagaagg ctatggagat aagaatgcga tttctgacaa
    19441 tgctattatt catggaagcg ctcagaacag aagaatcgag atccagtggt ttacaagccc
    19501 tgctcaaccc ccacaaccac aaatggctta tgtaaaataa aggtttatta atgcgttcaa
    19561 gatttatagg cggcatctta ttgatagttg gtacctccat tggtggaggg atgttggctc
    19621 taccagtagc taatgcagca accggttttt ggcaatcttc tctgtttctt cttatctgct
    19681 gggcctttat gacctttggt gcctttttca tcctggaagc aaatctctat ttgtccagag
    19741 gaaaacacat ggtttccatg gccaaggcaa ctctgggtga ttatgggtta cttactgcct
    19801 ggattagtta ccttttctta ttgtatacct tgctttccgc ttacatttcc ggcggcgctg
    19861 atgtattgaa cagtctattg tacaaagtca attggcatct cagtgattgg caggcaagtt
    19921 cgttatttac tttaattttt ggcctggtgg tatatggtgg catccattcc gtcgatgttg
    19981 ctaaccgcgc tctcatgttc ggtaaattag ccgtttactg catacttgtt atcctgattg
    20041 ccccacacat cgaatttcac tattttcaac atggtgatta tcgacagatt ggtggcgcca
    20101 ttatgatttt aattacttcc tttggttttg ctattatcgt gccaaatctg agagattatt
    20161 ttgatgatga cattaagaca ttaaaaaaag tcatttttat tggatcatta atacctttga
    20221 tatgttacat agcctgggat gccgtaatta ttggttcact cccttcagca ggcactagcg
    20281 gacttgaaag tctaatgcaa aatccgcgta caaccagcgc gttagccagt ttgttaactg
    20341 ataaagttga aaataccaca atcagcgccc tctttaattt ttttacctcc atttgcatgt
    20401 taactgcctt cttaggcgta tcactttgtt tatttagttt tcttgctgat ggcttaaaat
    20461 tgcgagagcg aggccgccat ggaattggct tatttgtttt gacttttttg ccgcctttac
    20521 taatcgttat ttattatccc ggggcttata tttatgctct gcgctacgcc ggtatatttt
    20581 gcatcatatt actactgtta ttgccagccc taatgtgtta ttttggcaga aagcgttaca
    20641 aatcaccctt ttgggttcca ggaggtaaaa tatcacagtt cgcagtgatt attatttctg
    20701 tggtcttatt ggcacatgaa tttattgcgc tttttaaata aaaatgatta atgttattac
    20761 tggcgttatt tttactttga tagcagtaaa acattaaaac ctgataaaca ggatgtgtaa
    20821 attgaacgcc agatcatttg cgaaaagaaa atgattgagg tctacaataa ggaagttgac
    20881 tttttttgag gaacaaagta tcattttgtt ctgtatttgt actgtttttg ggttatactt
    20941 cgtgatcaat tcaaaattaa ttggtggtat attactcata gtaggcactt cgattggtgg
    21001 tgggatgcta gcgctacctg tttctacagc cgaggtggga tttgctaact cattattttt
    21061 cctgtttttt tgctggttgg tgatgaccgc tggagcttta ttaattcttg aggtaaatct
    21121 acgactccct gcaggaagca atatgatttc aatggcgaaa tcaaccttgg gtctgccagg
    21181 tcaggtaatt gcctggataa cctaccttct tcttctttat accctcctgg cagcttatat
    21241 ttcaggtgga agtgatgtat ttagcagtct gctgcataag gcccatattg atttaccgaa
    21301 ttggataacg tctgtaatat ttactgccat gttcagcctt attgtatata gtggtatacg
    21361 ctctgtagac tacgttaatc gtggacttat gtttggcaaa ctgggcgtct atattttatt
    21421 ggttattatt atcagtcccc atgtatccac agcaggatta acaggcggct cagcgaaagc
    21481 ctttactggc actctcatga tcttggtcac ctcttttgga tttgcatcca tcgtgcccag
    21541 cttacgtgat tattttaacg atgatatacg tagcttacgt cgagttattt tgtttggttc
    21601 tcttatccca ttattctgct acatagtctg ggatgcagta attatgggcg ttatctcacg
    21661 cgagggagaa aatggtttat tgtccctaat gaactcggat catgcaacca gcggcctcac
    21721 tgaggcactt agtcaatcgg taagcagcca gtggatcagt ggattttttg gttttttcac
    21781 ttctatctgt atggttactg catttctggg agtttcatta ggattatttg attttcttgc
    21841 tgatgggtta aagcttaaaa aatctggtaa tcaaggcaag tatacgctgg ctctcacttt
    21901 tatccctccg ttggcagtgg tgttgtttaa cccgggtatc tacctgcatg cattaagtta
    21961 tgccggggtt tgttgtgtca ttttactgct gttattgcct gctatcatgg catggaaagg
    22021 aagacaaacc tgtagccaat cttcctcaga aactcatttg attcctggag gaaatccagc
    22081 tttaagcctt ataggattta tcgccattgg tttgcttatc attgcgatca tgtattaatt
    22141 aaagcctgaa tatcagcgtg aaaaagtaca
//
LOCUS       DBNCWK010000040        21715 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.040, whole genome shotgun sequence.
ACCESSION   DBNCWK010000040 DBNCWK010000000
VERSION     DBNCWK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21715
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.040"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            77..760
                     /gene="lem2"
                     /locus_tag="AC06IO_001359"
     CDS             77..760
                     /gene="lem2"
                     /locus_tag="AC06IO_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem2"
                     /protein_id="HIO9874559.1"
                     /translation="MVIIGILMSKERIKKTALEIVKYIHGNIKACNIPNQKKENKFFA
                     PLVYLCQTECDKILSNTKISLLERLLKCAKIIDDLQSGNCMQQAFLAFQRLLMQLVED
                     KLSDFSTCIPISIMTISNHAFLIIDNDIVCDPWLNFVGDLKDYCFAKMKRKEYFGIRS
                     DWTCFTNSEVYDEDSTEFTIQFVNALGVGISGFSEVKLANQLIQSSSMASAHGEDPVI
                     SIPKEFHRI"
     gene            868..1641
                     /locus_tag="AC06IO_001360"
     CDS             868..1641
                     /locus_tag="AC06IO_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9874560.1"
                     /translation="MNFLNNKIVMITGASSGIGQACARLFAAQGARLILAARRVERLH
                     ELSSELKHHHNQEHYILPLDVCKKDLVRQQIESLPSQWNSIDLLINNAGLALDTLPLH
                     QGILEHWDTMIDTNIKGLLYISRLILPGMLERNSGHVINIGSIAGHECYPNGNVYSAT
                     KHAVHAISKSMRLDMLGSAVRVTEIAPGAVETEFSEVRWKDKQRAIDFYKDFQPLMAE
                     DVADAALYCATRPLHVDIEQMIIMPTVQASANHLSRKGR"
     gene            2050..2280
                     /locus_tag="AC06IO_001361"
     CDS             2050..2280
                     /locus_tag="AC06IO_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874561.1"
                     /translation="MNKLIVAVTAGASLLLLTGCAVDGVAIDDVSYESGYSNDYVYSV
                     GYYSNRPYWGNNYYSSPSFVGPYGYWGGDYDF"
     gene            complement(2333..2902)
                     /gene="efp"
                     /locus_tag="AC06IO_001362"
     CDS             complement(2333..2902)
                     /gene="efp"
                     /locus_tag="AC06IO_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIO9874562.1"
                     /translation="MAVYSTNEFKNGLKVMVDDAPCSILDCEFVKPGKGQAFTRIKIR
                     NLKTGRVVERTFKSGDTLPSADVADVEMQYLYNDGEHWHFMVPDTFEQYAVTENILAD
                     AAQWLKEQDVCVVTLWNNEPIQVTPPNFVILAITETDPGLKGDTSGGGGKPATLETGA
                     VVRVPLFVQTGELIKVDTRKGEYVSRAKE"
     gene            2974..3954
                     /gene="epmB"
                     /locus_tag="AC06IO_001363"
     CDS             2974..3954
                     /gene="epmB"
                     /locus_tag="AC06IO_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HIO9874563.1"
                     /translation="MRDTSLSWQKILAQGFTSTTDLLDFLELPRSEGNLFAEKQFPSR
                     IPLGFAKRMQKGNPKDPLLLQVLAKEDELTEADDYVIDPLSESNTLIKGLLHKYHGRV
                     LLTLTGVCAVNCRYCFRRHFPYQANNPGRRGWKEVCAYIANDPSITEVILSGGDPLLA
                     ANLVLEELLQSLEEISHIHTLRIHTRIPIVLPERIDKGLLDLLTNTRFKKVIVIHCNH
                     PQELDESVLQACSDLKKAACYLLNQSVLLAGINDDAGILSKLSHALFDYGIMPYYLHL
                     LDKVKGSAHFDMPLPRAQSIYHQLQSLVPGYLLPRLAREEPGRSSKTLLI"
     gene            complement(4023..6095)
                     /gene="ppk1"
                     /locus_tag="AC06IO_001364"
     CDS             complement(4023..6095)
                     /gene="ppk1"
                     /locus_tag="AC06IO_001364"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIO9874564.1"
                     /translation="MDTTLDNPDYYINREFSAVAFNQRVLMLANDERVPLLERMRFLS
                     ICSSNLDEFFEIRVAGLKEKIAMSSGKLTIDGLRADEAFSQISHKAHALIDQLYNIFN
                     KQLLPALRKENIHFLETDQWTDDIHLWAKHYFKHEILPVISPIALDLAHPLPRLINKS
                     LNFIISLSGKDAFDRNINYAVVHAPRSIPRMIHLPSELCGDAHYFVYLSSIIKTHINS
                     LFPGMEISGCYSFRLTRNSDLFLREEEIDDLAKAVQREIFSRHYGHVVRLEIENKCPE
                     KIVDFLLQKYHLRHEDTYYCDGPVNLQRYMSAINSIDRPDLNYPAFTPQYPKFSKSQR
                     NLFNILDEQDILLHHPYQAFDLVIDFVRQAASDPNVLAINQTLYRTHSESVMVDALVN
                     AAHSGKEVTAVIELRARFDEESNLKLANKLHAAGILVLYGVVGYKTHAKMTLVVRRTH
                     GKLKRYVHLSTGNYHEQTAKRYTDLGLLTSEPTITSDAQLIFQQLTGLGKVVKLKALS
                     HSPFTLQKTLLQFIEQCSTAATGGIDTEIILKVNGLTDKVMIQALYKASQAGVKINLL
                     VRGVCCLKPGLPGVSENIRVLSFIGRFLEHHRVFYFRINEEEHYFCSSADLMERNLYN
                     RIEIMFPIYDEGCKKRIKNEIIKNYLKDNNNVWEMQSDGTYKHLMQNGGCAQEKLIAL
                     YEEEDKSI"
     gene            complement(6136..7341)
                     /locus_tag="AC06IO_001365"
     CDS             complement(6136..7341)
                     /locus_tag="AC06IO_001365"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIO9874565.1"
                     /translation="MKRIFFVVFLLCIETACNTAKNNPLPAQLADKALVPGFKEVRTE
                     FTRSDDPIIKKQSLERVKQLAKAYPDTMFHQKGSFSLLIISGGGDYGAFGAGLLNGWT
                     KSGSRPTFTTVTGISTGALIAPLAFAGPKYDATLKKVYTTITTKDVLSNKPVLWTLLT
                     GGSALKGTKPLQDLIARHVDEKLLDDIAKGYYQGRRLYVGTSNLYSRKLVVWDMTRIA
                     ASKNPERVQLFRKILLASASIPVVMPPVYFDVMANGKPYTEMHVDGSTTFAVFLSRLG
                     SDIKTAKKHFKLKQKPNVKIYVIRNNQNQFHYKVIKPKLLVIGKRAIESVTASQGAAD
                     VIFIYLCALIEGVDFNLANVPNEFDSSSEELFDTQRMNQLYQIGYSKAVKGYPWQKIP
                     PDVRALHGD"
     gene            complement(7417..7950)
                     /locus_tag="AC06IO_001366"
     CDS             complement(7417..7950)
                     /locus_tag="AC06IO_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIO9874566.1"
                     /translation="MIKTFIEIIYSFGKIGLISLGGGNSMLKLLEYEAVTYRHWIGKE
                     EFVSMLGSSFLFPGLTGIKLAALIGYKAAGITGLILAIICLNLPGLIMALVGYQWLNS
                     HNGPITRKIMISVQYGALALLAAATFSVVQGVIDVYYSIPMAICCILFFLALAFWNLS
                     PFYGFLAFITICFFVVR"
     gene            complement(7947..8567)
                     /locus_tag="AC06IO_001367"
     CDS             complement(7947..8567)
                     /locus_tag="AC06IO_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874567.1"
                     /translation="MQRLVWNFEFSSEQTTPLTTLGHDAQDNLKWEKRYFWPTDQIIR
                     LNTIDSSLLDLANYQKKHKEDYYYLLPDSDHNIKQRRNELLYKPVIKQSGSALGFGSK
                     IHLETPQTLPCHGDAGQLNRQELLKKIKEKGIVVFVKKEAFVFKFPTTPTVKLELARL
                     EVKDKVYFSACIEGRSLSLVETISVLLFGTQPSFEYVTFLKKTLKI"
     gene            8743..11181
                     /locus_tag="AC06IO_001368"
     CDS             8743..11181
                     /locus_tag="AC06IO_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIO9874568.1"
                     /translation="MAIVFFLIYLAFTLVVLSRRINPLVWEIGSIIYLIPATFYFGFP
                     WIIGLFIWFFIIAAVLVVRVEPLRLFIGDYLYKTAGKSVPKLSKTEEEALNAGDTWLE
                     QDIFTGKPDWQRLSAVSTELTAEEQAFLDNETHTLCSMLDEWEISQEYDLSPKVWNYI
                     KEKGFLGLVIPKEFGGKGFSARAHSDIVMKIATRSRVAAVTVMVPNSLGPGELINYYG
                     TDEQKAYYLPRLAKGVEIPCFALTEPGAGSDATSIQSEAIVLKKKIDGKSVLGLNITL
                     DKRWITLAPVATLIGLAVNLKDPDGLLNGEGEEGITCLLIPRDTENLEIGNRHLPADQ
                     PFMNGTLRGKDIFVPIDTIIGGQKKAGSGWQMLVECLSIGRSISLPALGAGSSSISYL
                     TTGAFARIRRQFNVEIAQFEGVEEKLAEIAGLNYLINSTRLLTVAAVNEHKKPSVASA
                     ITKYFNTELARITVNASMDVHAGRAIVVGPRNYLNSLYQSVPISITVEGANIMSRNLL
                     IFGQGSMACHPFIRDEFYAISSENKEAFREIIWKHIHYFMQNFAKTICSAWTSGLFIA
                     VPENSMKREYKKLARLSYAYSWLADLSLIYLGGDLKRKERLSARLADGMSYLYMAMAA
                     LRNVQLNQDHPDEQLHARWAVSYCFYHAQKSMIALCHNFPSKFLGFIARVVAFPFGQT
                     MRYPSDKLDHQLARLMTGNNHYRDRLKKSVFLSGDSKQPVDRMEHALQLIIEADGLSK
                     KVSDLKRYKWSKLKEKLKEKVESGQLSQQEMEELLAVEDARWDAILVDEFTFDSMKKK
                     TLHSVIEKMKSPLA"
     gene            11295..11987
                     /locus_tag="AC06IO_001369"
     CDS             11295..11987
                     /locus_tag="AC06IO_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0294 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874569.1"
                     /translation="MPKYTLPTRDALLKAMQVGETSIEAAEYMATRFEQILTKAKLLP
                     ECNDMLEKIKEYAQFVKFKLLSSAQVWSGQERPTSDYQNTQENKAEFLASHLEGLPSG
                     LKLEVAIGDDAKILRGFSSNGKMVEGDQLKTMDGLLEGWLAKNGLAISGGAVVKIDNT
                     GNQTKVDPQEIRQLINDSEKGVAKYFADKGVGMEVAQRTYQEPKALETKREEIRQEIE
                     SGAEAPTTQSIR"
     gene            complement(12024..12686)
                     /gene="murU"
                     /locus_tag="AC06IO_001370"
     CDS             complement(12024..12686)
                     /gene="murU"
                     /locus_tag="AC06IO_001370"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HIO9874570.1"
                     /translation="MKTAMILAAGRGERLRPLTDKIPKALCTVRNKPLIEHHIINLAN
                     AGFERLIINHAYLGGQIRQYIGNGKQWGLNVIYSPEPPGGLETGGGIVNALPLLGEEP
                     FLTVNADIYTDFDFAKLQLKNIDTFHLLLIPKNPSLLHHGDFGLINGSHLTNTNQAYT
                     FSGIACYNPKVFANCRQGRYSVTPLLRKYVEQNSATGSVHSGIWYDIGSFDRMQAANQ
                     FT"
     gene            complement(12683..13660)
                     /locus_tag="AC06IO_001371"
     CDS             complement(12683..13660)
                     /locus_tag="AC06IO_001371"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIO9874571.1"
                     /translation="MHERENALKEWLAQTIQQKDFVLLPLAGDASFRRYFRIQYNGLT
                     QVVMDAPPEKENIEPFLHIANVLDKIKIPVPDILAINKREGFLLLSDLGDQLLLNKLN
                     HETVDNYYNQAMNLLLQIQKCPTDDPELPLFDKSFMLKEMNLCLEWFLKAYLSLDLKQ
                     DELQLFQHTIEWIASEVATQPGVFIHRDYHSRNLMLVKNKDSCLATIDFQDAMLGPVT
                     YDLVSLLKDCYISWPREKVLEWVTYFHEQSPLASIYSLTDFIRAFDLCGLQRHLKVLG
                     VFCRLYLRDNKAGYLGDLPLTLKYALECAETYEELHPFFNFLQKRVYLP"
     gene            13903..16422
                     /locus_tag="AC06IO_001372"
     CDS             13903..16422
                     /locus_tag="AC06IO_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIO9874572.1"
                     /translation="MPIGITACVLSLINYQGLAYSAALINEPIQACVIAREVDLTDDI
                     RTKFAQCLGWQADQSSPVCLGFYKPITVTPLASPDEVRILADTASFYRTQRSTLSGHV
                     EMQQGQRVVNAQTAYVYRDPKTNEVTKIEFLNHVRYLEPDRMMIARKAVVYPQDKSGE
                     VEDVLYRFNTNRSNALLPAWGRASLIKRFANQDYFLKEATYTTCAPQDKAWAIEAESI
                     SIDNEKGKGIARNAKLRIHEWPVLYTPYLSFPTNRDRKSGFLMPIVGYSNVGGADLGI
                     PYYWNMAPNYDMTLVPHLYTKRGLMLGGQFRYLTSKSTGTFNGNFLPKDKAFGRFLQD
                     NEVEFPQIRGLSTNRWEVNFVDSTQFLSDLQLNVNFQQVSDDYYLQDFSTNLASVTQR
                     QLLRQADLTYTTENWTFRGMGQSYQTLHPINEIPVSPVYERLPQLMARGYYDDLPFNA
                     QLNILGQYDQFHWPNDSWNIALNNMPQGPRFHLNPILSVPMMKPWGYVTPSVQFVENY
                     YDISRNYTWGTSRANYNLTIPRYSLDGGLYFERDLHLKGTYYIQTLEPRLFYLRVPYY
                     NQTLIPVYDSGFMIFNVDQLFRTNRFSGFDRIGDANQLSYALTTRWLEDESGAEKANF
                     SIGQIKYFSERRVQLCQSPTGFCTDNPDTFGNLSSTFGTSPVASRAVYKFNPAWGITG
                     DYIWDPATRATNNADLNLHYQPARNAIINGGYSYLVNGDVTQVRNNDTENNALHQAIL
                     SAAWPLSEKWSGIGAYSYNISKNYSMMSFLGVQYDSCCWAMRILGGRTFRSLNEEFEP
                     QYNNNIYLQILLKGLGSVASSDPSGILNTYIPGYYDPFRRR"
     gene            16598..17887
                     /locus_tag="AC06IO_001373"
     CDS             16598..17887
                     /locus_tag="AC06IO_001373"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIO9874573.1"
                     /translation="MFKRIALVCALFSGVCFAEGKQLLDKVVAIVNDNVITSSELNAQ
                     VELSKKQIIAQNMQMPDESVLRKQVLQHLIDVDLEMQMAKQNGITIENAEIDEAIEKI
                     AASNHLNLSQMRDEITKQGISWQEYRQNIRKEMLISRVQQKAVGKDIIVTNEQVEQYL
                     KTAGRIENSNLTYHLKNIVIPLSEEPTTKQLQRAKIEAENLLNKIKKGEDFSRLAIEE
                     SSGEFALEGGDLGERHLAELPEVFAKEVVHMKVGQVAGPIRAGNGFHLIKLVAVGGEN
                     QRHVITQTHVRHILLKPDASMVPSEAIKQVNNIYRQIQSGKDFALMAKQYSLDAASAV
                     KGGDLGWVNPGELVPEFEKTMNSLPLHKVSKPVKTQYGWHLIEVIARRQKDDSEAFKK
                     QQVRQFLQQRKFVEAVQNWQQHLRSQAYINIVDKDLA"
     gene            17884..18858
                     /gene="pdxA"
                     /locus_tag="AC06IO_001374"
     CDS             17884..18858
                     /gene="pdxA"
                     /locus_tag="AC06IO_001374"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIO9874574.1"
                     /translation="MNPLLISSGEPAGIGPDLCLALAETDLPVVILGDLSLLEARASE
                     LNLSIKFLEYSPHQSFEKKAGYLTVWPVPCAAPVISGELNPRNAAYVMELLTLGASLC
                     SKGEFSALVTAPVHKANINAAGITFTGHTEFFADFFEVETVVMMLACSQMKVALVTTH
                     LPLRMVPDAISSLLIVKVIQQLHHSLKHDFGIQSPKINVAGLNPHAGESGYLGREEIE
                     IITPALNTLKNQGIDVQGPLPADTMFITNHINHCDAYVAMYHDQGLPVLKYAGFNEAV
                     NITLGLPIIRTSVDHGTALELAGKNKANPGSMLAAVKMAKDMALTRIK"
     gene            18860..19351
                     /locus_tag="AC06IO_001375"
     CDS             18860..19351
                     /locus_tag="AC06IO_001375"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIO9874575.1"
                     /translation="MSLISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIM
                     GRKTYESIGKPLPGRLNVVISQSIQSIPGVVVVDSLGQAIKETADASEVMIIGGAKIY
                     SQAISMANRLYITRIHHQFTADVFFPKINELAWCCRSKEYRQHDDKNQYDMTFCVYER
                     ISI"
     gene            complement(19358..19903)
                     /locus_tag="AC06IO_001376"
     CDS             complement(19358..19903)
                     /locus_tag="AC06IO_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HIO9874576.1"
                     /translation="MSKLVYLLLMIFFLNPAWANTHYHQHPVSPLANALRTGTDTNGN
                     ALYLCKAKLFNSIQPGKTWAGYGRCNVPYGGKEYVLSQFTIPNQNEFGRYSWEPNVEH
                     ALLMGKDTNGNPLFVCQSNFNGSIQPGKTWPGYSHCNISYGGREIITDNYRILSRPTE
                     IIVRTQPPVHRHPSQNRHYHY"
     gene            20189..>21715
                     /locus_tag="AC06IO_001377"
     rRNA            20189..>21715
                     /locus_tag="AC06IO_001377"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6538 a         3982 c         4686 g         6509 t
ORIGIN      
        1 gggatgtatt tttgtcaaat tgtaagcgat ttttgattta aaaaaggtat aatagatgac
       61 aaaataaaaa ccatctgtgg ttatcattgg aatattgatg agcaaagaac gaattaagaa
      121 aacagcactg gagattgtca aatacattca tggcaacata aaagcgtgta atatccccaa
      181 tcaaaaaaaa gaaaataagt tttttgcgcc tttggtttat ctctgtcaaa cagaatgcga
      241 taagattttg agtaacacca aaatcagttt gttggagcgt cttttaaaat gcgcaaaaat
      301 tattgatgat ctgcaatcag ggaattgtat gcagcaagcc tttttagcat ttcaacgttt
      361 attgatgcaa ttggttgaag ataagctatc tgatttttcc acttgcattc caatttctat
      421 tatgaccatt tccaaccatg cctttttaat cattgataac gatatagtgt gtgatccctg
      481 gttaaatttt gtgggagatt tgaaagatta ttgctttgca aaaatgaaaa ggaaggagta
      541 ttttggaata cgttccgact ggacttgttt taccaattca gaggtctatg atgaagactc
      601 tactgaattt acaatccaat ttgtaaatgc tttaggggta gggatatcgg gtttcagtga
      661 agttaagttg gcaaatcaat tgatacaatc atcgagtatg gcttctgctc atggagagga
      721 tcctgtgatt tctatcccca aagagtttca tcgaatttga tgaagcgctc atcttgattg
      781 taatgaactc tattgctgtt gtttttgttc aagttctggt tcgatttaac ggtaaatata
      841 ttgtacttga taacgatggg ggagaggatg aattttttaa acaataaaat tgtaatgata
      901 accggcgcgt ccagcggcat tggacaggct tgcgcccgac tttttgctgc acaaggagca
      961 cgactcatct tggcggcacg acgggttgaa cgtttacatg aattatccag cgagttaaaa
     1021 catcatcata atcaagaaca ttatattctt cctctggatg tctgtaagaa agatttggta
     1081 agacaacaaa tcgaatcttt gcctagtcag tggaactcta ttgatttgtt aatcaataat
     1141 gcgggtttgg ctctggatac cttacctttg catcaaggaa tattggagca ttgggatacc
     1201 atgatagata ccaatattaa aggattgctt tatattagtc ggttaattct cccagggatg
     1261 ttagaaagga atagtgggca cgttattaat atcggttcca tagcaggtca tgagtgttat
     1321 cctaatggaa atgtttattc ggctaccaag catgctgttc atgcgatatc aaaatccatg
     1381 cgactggata tgttaggaag tgcagttcga gtgactgaaa ttgcaccagg tgctgttgag
     1441 acagagttta gcgaggttcg ctggaaagac aaacagcgag ctatcgattt ctataaggat
     1501 tttcaaccgc ttatggccga agatgttgct gatgccgctt tgtattgcgc cacaagacct
     1561 ctgcatgtcg atattgagca aatgattatt atgccaacag ttcaggcttc tgcaaatcat
     1621 ttgtctagga aaggcagata atcatcaggc gaataattgc gcaaaaattt gccaataatg
     1681 gggctgttag tctattattt aaaatatagg tgatgactga tcctggctga accagtaagt
     1741 agtgccagat taggaggtcg ttttggaggg tggtgtgtaa gcctacaaag ggtaggaaac
     1801 ctgaagctct acataaatct ccacagtgtc tttcagttgc ggctcagaaa ccgggaagtc
     1861 gttgcctgta cttaaactct tttctattct ctgtataaaa taattttttt cttatctaaa
     1921 gaatgactta agttaaacag atcatgcgca accccataac ctcgacattt attcttaaaa
     1981 aagaacaatt tgagcaatta ataatattat cttataatta aagaacaact attttgccag
     2041 gaagcaatta tgaacaagtt aattgttgcg gtcactgctg gtgcatcatt attgctgcta
     2101 actggatgtg cggttgatgg tgtggctatt gatgatgttt cttacgaatc aggttatagc
     2161 aatgactacg tttattcggt tggatattat agcaatcgcc cctattgggg taacaactac
     2221 tattcgtcac cttcttttgt aggcccttat ggatattggg gtggagatta cgatttctaa
     2281 tttccagaga agacaagcct tcaaaccaaa tgaaggctat tcctgtggat aactactctt
     2341 tagcgcgaga cacatattcg cctttgcgcg tgtctacctt aatcagttcg ccagtttgaa
     2401 caaacaatgg tacacgaacc acggcacctg tttccaatgt ggctggcttt ccacctccgc
     2461 cagaagtgtc tccttttaat ccaggatctg tttctgtgat tgccaaaatg acaaaattgg
     2521 gtggagtcac ctgtataggt tcattgttcc atagagtaac tacacaaacg tcctgttcct
     2581 ttaaccattg tgctgcatcg gctaatatgt tttcagtaac ggcatattgt tcaaacgtat
     2641 ctggaaccat aaaatgccaa tgttccccat cattatatag atactgcatt tctacgtctg
     2701 ccacatcagc agatggcaag gtgtccccag atttaaaggt acgttccaca acgcgcccgg
     2761 tttttagatt gcgaatttta atacgggtaa atgcttgacc ttttccgggt tttacaaatt
     2821 cacagtcaag aatgctgcac ggagcatcat caaccattac ttttaagccg tttttaaatt
     2881 catttgtgct atagactgcc atttctgctc catagttgag attttgctgt attttcgcta
     2941 aagttcgcac agtttatcaa gtctgtaact ttaatgcgag atacctcttt gagttggcaa
     3001 aaaattttgg cgcaaggatt tacttcaacg actgatttat tagatttcct ggaattgccg
     3061 agatctgaag gaaacctatt cgcggaaaaa caattcccaa gccgtattcc tttaggtttt
     3121 gctaagcgaa tgcaaaaagg gaatcccaaa gatcccttgc ttttacaggt tcttgcaaaa
     3181 gaagatgagc ttactgaagc agatgattat gttatcgatc ctctgagtga gagtaatacc
     3241 ctgattaaag gtttattgca taaatatcac gggcgtgttt tgttaacttt aaccggtgtt
     3301 tgcgctgtga attgtcgtta ttgctttaga agacattttc cttatcaagc gaataatccg
     3361 ggccgccggg gatggaaaga ggtgtgtgcc tacatcgcaa atgatcccag tattactgaa
     3421 gttattttaa gtggtggcga tccattgctc gcagccaatt tggttttgga agaattactc
     3481 caatctttgg aagaaatttc tcatatacat actttaagaa ttcatacaag aatacctatt
     3541 gttttaccgg agcgtattga taaaggatta cttgatttat taacaaatac gcgatttaag
     3601 aaagtgattg ttattcattg taatcatccg caagagttgg atgagagtgt attgcaggca
     3661 tgctctgatt tgaagaaagc agcatgttat ttactgaatc aatctgtttt attagccggg
     3721 attaatgatg atgcagggat tttatccaag ttaagtcatg ctctttttga ttacgggatt
     3781 atgccttact acttgcattt gctcgataaa gtaaagggtt cggcacattt cgatatgccg
     3841 cttcccaggg ctcagtcaat ttaccatcaa ttacaatccc ttgtgccagg atatttattg
     3901 cctcgcctgg cacgagaaga gccgggaaga tccagtaaaa ccttgctgat ttaaagagct
     3961 tggtatgtct tggctattta tggttttatt caatagccat gttttcactg ttatttttct
     4021 gattaaatcg atttgtcctc ttcctcataa agtgcaatca gcttttcctg agcacagccg
     4081 ccgttttgca tgaggtgctt gtaagtcccg tcactttgca tttcccaaac attattatta
     4141 tctttaagat agtttttaat gatttcgttt tttattcttt ttttacatcc ctcatcataa
     4201 atggggaaca tgatttcaat acgattgtat aaatttcttt ccatcagatc ggcactggag
     4261 caaaaatagt gttcttcttc gttgatgcga aaataaaaca ctctgtgatg ttcgaggaat
     4321 ctgccaataa aagacaagac tctgatgttc tcagacacac caggcaatcc tggttttaaa
     4381 caacataccc cacgtacaag aagattaatt ttaacgcctg cctgtgatgc cttatataat
     4441 gcttgaatca tcactttatc agttaatcca ttcactttca ggatgatttc agtatcaatg
     4501 cctcccgtag cagcagtact gcattgttcg atgaattgta aaagagtttt ttgtaaggta
     4561 aatggcgaat ggctcaaggc ttttaactta acaacctttc caagaccggt taattgttga
     4621 aaaatgagct gggcgtcaga agtaatcgtg ggttcactgg ttaataatcc caaatcagtg
     4681 tatctcttgg ctgtttgttc atgatagttt cctgtgctta agtgcacata tcgttttaat
     4741 ttaccatgcg ttcttcgaac gaccagcgtc attttcgcgt gagttttata gcctactact
     4801 ccgtagagta ccaggatccc cgcggcatgg agtttgttag ccaattttaa gttggattcc
     4861 tcgtcaaaac gggctcgtag ctcaatgaca gcggttactt ctttccctga atgggcggcg
     4921 ttaaccagcg catcgaccat cactgattca gagtgggttc tgtaaagcgt ttgattaatt
     4981 gccagtacat tagggtctga agcagcttgc ctgacaaaat caatgactaa atcaaatgct
     5041 tgataaggat gatgtaataa aatgtcttgt tcgtcgagaa tattaaataa attacgttgt
     5101 gatttggaga acttgggata ctgtggggta aaagcgggat aattcagatc aggtctatcg
     5161 atgctgttaa tagcactcat atatcgttgt aaattaacag ggccatcaca ataataggtg
     5221 tcttcatgtc gcaaatgata tttctgcagg aggaagtcaa caattttttc agggcatttg
     5281 ttttcaattt ccaatctcac gacatggcca taatgacggg aaaatatctc tctttgcacc
     5341 gctttagcca aatcgtcaat ttcttcttct cttaaaaata aatcactatt tcgcgtcaac
     5401 cgaaatgaat aacatccgct tatttccata cctggaaata agctattaat atgcgtttta
     5461 ataatggaag ataaataaac aaaataatgg gcatctccac aaagctcgga aggcaaatgt
     5521 atcatgcgtg gaatcgaacg gggagcatgt acgacggcgt aattgatatt tcgatcaaaa
     5581 gcatctttcc cactcaatga aatgataaaa tttaaacttt tattgataag ccttggaaga
     5641 ggatgggcca aatccagagc aatagggctt ataacaggta gaatttcatg tttaaagtaa
     5701 tgctttgccc ataaatgaat gtcatcagtc cattgatctg tctcaagaaa atggatattt
     5761 tctttgcgca atgccggaag tagttgtttg ttaaaaatat tgtaaagctg atcgattaaa
     5821 gcatgagcct tatggcttat ttggctgaat gcttcatcag cccgtaatcc atctatggtt
     5881 agcttgcctg acgacatggc aattttttct ttcaacccgg ccacccgaat ttcaaaaaat
     5941 tcatcaagat tactgctgca gatactgaga aatctcattc tttccaataa gggtacacgt
     6001 tcatcattag caagcatgag tactctctga ttaaacgcaa cagcagaaaa ttctctatta
     6061 atataataat ctgggttatc cagagtagta tccatgagga actccaggta ttttcatttc
     6121 atgttcacag gcaatttagt caccatgcaa agcccttaca tctggaggga ttttttgcca
     6181 ggggtaacct tttaccgctt tggaataacc aatttgatat aactgattca ttctttgcgt
     6241 atcaaacaat tcctccgagc tcgaatcaaa ctcatttgga acattggcca aattaaaatc
     6301 aactccctct ataagcgcac aaagatagat aaaaataaca tcggcagccc cctgggatgc
     6361 cgtgacactt tctatcgctc gcttaccaat taccaataac ttgggtttga ttactttgta
     6421 atggaattga ttctggttat tgcgtattac ataaatttta acgtttggtt tttgttttaa
     6481 tttgaaatgt tttttcgcgg ttttgatatc agaaccaagg cgacttaaaa agaccgcaaa
     6541 cgttgttgag ccatcgacgt gcatttctgt gtagggtttc ccgttggcca tgacatcaaa
     6601 ataaacaggc ggcatgacca cgggaatgct ggcagaagca agtagaattt ttcggaataa
     6661 ctgaactctc tcggggtttt tgcttgccgc aatgcgggtc atatcccata ccaccagctt
     6721 gcgagagtaa agattggatg tccctacata aagccgtcgc ccttgataat agcctttggc
     6781 aatgtcatca agcaattttt catcgacatg acgagcaatc aggtcttgta atggtttggt
     6841 tcctttaagg gcgctgcctc ctgttaacag ggtccataat acaggtttat tactcagcac
     6901 atccttcgtg gtaatggttg tatacacttt ttttaatgta gcatcatatt tggggccagc
     6961 aaaagctaaa ggtgctatca atgccccagt acttatacct gtcacggttg taaatgttgg
     7021 tcgagaaccg gattttgtcc acccattcaa taatccagcg ccaaaagcac cataatcgcc
     7081 accacctgaa ataatcagca agctgaaaga gcctttttga tggaacatgg tatcagggta
     7141 agctttagcc agttgtttta cacgttcaag agattgcttt ttaattattg ggtcatcact
     7201 gcgggtgaat tcagtgcgta cctctttaaa accaggcact aaggccttat ctgccaattg
     7261 tgccgggaga ggattatttt ttgctgtatt gcaagcggtt tctatacaaa gcaaaaatac
     7321 aacaaaaaaa atccttttca taattttcct ttaaattaat gttttcccga attttattca
     7381 aaaaatggat taaaagcgat agtgtttgtt ttcatattaa cgcaccacaa aaaaacatat
     7441 ggttataaat gctaaaaaac cataaaaggg tgatagattc caaaaggcca aggcaagaaa
     7501 aaacaaaata cagcaaatag ccataggaat agaataatag acgtcaataa caccttgaac
     7561 tactgaaaat gtagcagccg ctaacaaggc taaagctccg tattggactg agatcataat
     7621 tttgcgtgtt attggaccgt tatgagaatt aagccactga tatcccacaa gagccataat
     7681 taatcctggt aaattcaggc agatgatcgc aagaattaaa ccggttattc cggccgcttt
     7741 atagcctatt agtgcggcca gttttattcc cgttaatccc ggaaataaaa aacttgaacc
     7801 taacatactt acaaattctt cttttccaat ccagtgcctg tacgtgactg cctcatattc
     7861 caataatttt aacatggaat tgccgccgcc taaggatatc aggccaattt tcccgaaact
     7921 gtaaatgatt tcaatgaaag tttttatcat atttttaaag tcttttttag gaaggtaacg
     7981 tattcaaaag aaggttgggt tccgaataaa aggacgctga tggtttctac caggcttaat
     8041 gatctcccct caatacaggc actgaaatac actttatcct tgacttcaag acgagcaagc
     8101 tcaagcttga cagttggtgt tgtagggaat ttaaaaacaa aagcttcttt tttaacaaaa
     8161 acaacgattc ctttttcttt tatttttttc aatagttctt gtcgattcaa ttgcccagcg
     8221 tcgccatggc aagggagagt ttgtggggtt tcaagatgaa ttttagatcc aaagcctagt
     8281 gcagagccag attgcttaat aacaggttta tacaataatt cgttacgtct ttgttttata
     8341 ttatgatcag agtcaggcag taaataataa tagtcttctt tatgtttttt ctgatagttg
     8401 gctagatcaa gcaacgaact gtcaatagta tttaaacgga tgatttgatc tgtaggccaa
     8461 aaatagcgct tttcccattt tagattgtct tgagcatcat gacctaaagt ggtaagtggt
     8521 gttgtttgtt cagaggaaaa ttcaaagttc caaaccagtc tttgcattat tagtatttca
     8581 ttttctgttt tgcttacgct agatgatttg atttgttttg gcaaggttaa cttaaatgtt
     8641 tcttataatt tttaaaggat tattgctcgc taaataaagc gtaaaaagta tatactggtt
     8701 attctataaa cctcttaata aaatagcaaa ggaaggtatt ttatggctat tgtgttcttt
     8761 ttaatttatt tggcttttac tctggtagta ctgtccagaa gaattaatcc attggtttgg
     8821 gaaattggaa gcataatcta cctcattccc gcaacatttt attttgggtt cccctggatt
     8881 atcggtttat tcatttggtt ctttattata gctgccgtgc tggtagttcg agtggagcca
     8941 ttacgccttt tcatcgggga ttatttgtat aaaaccgcag gaaaatcggt gcccaagctc
     9001 tctaaaacgg aagaagaagc tttaaacgcg ggggatacct ggctggaaca agatattttt
     9061 acaggaaaac ctgattggca aagattatca gcagtttcta cagagctcac agcagaagaa
     9121 caagcttttt tggataatga aactcatact ttgtgcagta tgttggatga atgggaaatt
     9181 agtcaggaat atgatttatc tcccaaggtt tggaattaca ttaaagagaa aggattttta
     9241 ggattagtga taccaaagga attcggtggc aaagggttct cagctcgagc ccattccgat
     9301 attgtaatga aaattgcaac tcgctcaaga gtagcggcag tgacggtaat ggttcctaat
     9361 tccctgggcc cgggtgagct aataaattat tatggaaccg atgagcaaaa agcatattat
     9421 cttccacgtc ttgccaaagg tgttgagatc ccctgttttg cattgacaga gccaggggct
     9481 ggaagtgatg cgacctccat ccagtcagaa gccattgtac ttaaaaagaa gatagatggg
     9541 aaatcagtac ttggcttaaa tatcaccctg gataaacggt ggattactct agctcctgtt
     9601 gcgactttaa ttggattagc cgtgaacttg aaagatccgg atgggctttt gaatggtgaa
     9661 ggcgaggaag gcataacttg cttgctgatt ccaagagata ctgaaaatct tgaaattggc
     9721 aataggcatt taccagctga tcagcccttt atgaatggaa ctttacgtgg caaagatatt
     9781 tttgtgccta ttgataccat cattggtggt caaaagaaag cggggtccgg ttggcaaatg
     9841 ttggttgagt gcttatctat agggcgctca atatctctgc cagcacttgg agcaggttct
     9901 tcttctattt cttatttaac aacaggtgct tttgccagaa ttcgtcgcca atttaatgta
     9961 gaaatcgctc agtttgaagg agtagaggaa aaacttgcag aaatagcggg tttgaattat
    10021 ttaatcaatt ctacccggtt gttgacggtt gctgcagtga atgaacataa aaaaccttct
    10081 gtagcttcag cgattaccaa atattttaat acggaattgg cacgaatcac tgtgaatgct
    10141 tctatggatg ttcatgctgg gcgagccatt gttgttgggc cacgcaatta cttaaatagt
    10201 ttatatcaaa gtgttcctat ttctattacg gtagaagggg ctaatattat gtctcgcaat
    10261 ttattgattt ttgggcaggg atctatggcg tgtcatccgt ttattcgtga tgaattctac
    10321 gccatctcca gtgagaataa agaggcattt agagagataa tttggaagca tattcattat
    10381 tttatgcaga attttgctaa aacaatctgt tcagcatgga caagtggttt gtttattgcg
    10441 gttcccgaaa attcaatgaa aagagagtat aagaaactgg cgcgtttgag ttatgcctat
    10501 tcctggcttg ctgatctgtc tttaatttat ttgggaggtg atttaaagcg caaggagcga
    10561 ttgtctgcaa gattggccga tggcatgtct tatctctata tggcaatggc tgctttacga
    10621 aatgttcaat taaatcaaga ccatcccgat gagcaattgc atgccagatg ggcagtaagc
    10681 tattgttttt accatgcgca aaaatccatg attgcacttt gccacaattt cccttccaaa
    10741 tttttaggtt ttattgcccg agtagttgcg tttccatttg ggcaaaccat gcgttatcct
    10801 tcagacaaat tagatcatca gttggctcgt ttgatgacgg gcaacaatca ttatcgtgac
    10861 cgtttgaaaa aatcggtctt tctcagtggt gattccaagc agcctgtgga tcgtatggaa
    10921 catgctttac agcttattat tgaagcggat ggattaagca aaaaagtgag tgatttgaag
    10981 cgctataagt ggagtaagtt gaaagagaag ttaaaagaaa aagtcgagtc tggccagttg
    11041 tcacaacagg aaatggaaga gttgcttgct gttgaagacg cgcgttggga tgccatttta
    11101 gtggatgaat ttaccttcga ctccatgaag aagaagacat tgcattccgt gatagaaaaa
    11161 atgaaatctc ccttggcgtg atttttacag acttttaaca tcgatttacc aaccagctat
    11221 caatccaaat gaggctggtt tgctataatt aaactatatt gcctggacca aatatacatt
    11281 gttgagagta agttatgcca aaatacacac tgcccacaag agatgcctta ttaaaagcga
    11341 tgcaagtagg tgaaacatcc attgaagccg ctgaatacat ggcaacaagg tttgagcaga
    11401 ttttaacgaa agcgaaatta ttaccagaat gtaatgatat gctggaaaaa ataaaagaat
    11461 atgctcaatt cgttaaattc aaattattat cctctgctca agtttggtct ggccaagaaa
    11521 ggccaacttc agattaccaa aacacgcagg aaaacaaagc cgaatttttg gcaagccatt
    11581 tggaagggtt accaagtgga ttaaaactcg aagttgctat tggggacgat gccaaaatat
    11641 tgcgagggtt ttcttctaat ggaaaaatgg tagaaggaga tcagttgaaa accatggatg
    11701 gattattaga aggatggctt gccaagaatg gtcttgctat ttcaggaggt gcggttgtta
    11761 aaatagacaa tacgggcaac caaaccaaag tagatccaca ggaaatcaga caattaatca
    11821 acgatagcga aaagggagtc gccaaatatt ttgcagataa aggcgtgggc atggaagtgg
    11881 cgcaacgaac ctaccaagaa cccaaagcat tggaaaccaa gcgagaagaa atcagacaag
    11941 aaattgaatc cggtgctgaa gctccaacca ctcaatccat cagataggaa tatactggtt
    12001 tacctaaaaa ccaatcttgg tttttaggta aactgatttg ctgcctgcat tctatcgaaa
    12061 gagcctatgt cataccaaat tcctgagtga acacttccag tggcactatt ttgttccaca
    12121 tattttctga gtaaaggggt aacagaatac cttccttgtc tgcagttggc aaaaaccttt
    12181 gggttatagc aggcaatacc tgaaaaagtg taagcctgat ttgtattggt taagtgggat
    12241 ccattgatta aaccaaaatc gccatgatga agcaaagaag gatttttggg aattaaaagc
    12301 agatgaaatg tatcaatatt tttgagttgg agcttggcaa aatcaaaatc agtataaatg
    12361 tcggcattta ctgttaaaaa tggctcttcg cccagcaaag gcaaggcgtt cacaatccct
    12421 ccccctgttt caaggcctcc tggcggttct ggagaataga taacatttaa gccccattgt
    12481 tttccatttc ctatatattg gcgaatttgt ccgcccaggt aggcatgatt gattatcaat
    12541 cgttcaaagc cagcattggc gaggttaatg atatggtgtt caattaacgg tttgtttctt
    12601 accgtacaaa gtgctttagg aattttgtcg gttaaagggc gtaatcgttc tccacgtcct
    12661 gctgcaagaa tcatagcggt tttcatggta aataaactcg tttttgcaga aaattaaaaa
    12721 atgggtgtaa ttcttcatat gtttctgcgc attctaatgc atattttaac gtgagaggca
    12781 agtccccaag atatcctgct ttattatcgc gcaaatataa acggcaaaaa acacctaaca
    12841 cttttaaatg cctttgtaac ccacatagat caaaggcccg gataaaatcg gttagagagt
    12901 aaatactggc gaggggggat tgttcatgaa aataggttac ccattctaaa actttttctc
    12961 ttggccatga aatataacaa tctttgagta aagaaaccaa atcataggta acaggaccta
    13021 gcatagcatc ctgaaaatca atagttgcta aacagctgtc cttatttttg actagcatca
    13081 gatttcttga atggtagtcc ctgtgaatga aaactcctgg ttgagtcgct acttcacttg
    13141 cgatccactc gatggtatgc tgaaaaagtt gtaattcgtc ttgttttaaa tcaagagaaa
    13201 ggtaagcttt caaaaaccat tccaaacata aattcatttc tttaagcatg aaacttttgt
    13261 caaataaggg aagttcagga tcgtcagtgg gacatttttg aatttgtaat agcaaattca
    13321 ttgcctggtt ataataatta tccactgttt catgattcag cttatttaat aataattgat
    13381 cccctaaatc gcttagtaac aggaaacctt ctcttttatt tattgccaga atatcgggga
    13441 ctggtatttt aattttatca agaacgttag cgatatggag gaaaggttca atattttcct
    13501 tttcaggtgg agcgtccata actacttgag ttaatccatt atattgaatg cgaaaatacc
    13561 ttctaaaact tgcatctcct gctaaaggaa ggaggacaaa gtctttttgt tgaatcgttt
    13621 gagctaacca ctctttcagt gcgttttctc tttcatgcat ggtatacttc ccgtttttat
    13681 ttacagagtg cggttgatat actcccttct taataaagag taatttcatt ctcgcacaaa
    13741 taataaggta ttttgttagc tgtgaacatg aggttttata aaaagctaat tatatccggt
    13801 tttatttcat ttatcgcctt attgcgaggt ttactctatt gccatttaaa aagaccattt
    13861 aaaaaatggc ctgaatcttt attttcagtg aaatgggttt taatgcccat agggataact
    13921 gcttgtgttt tatctttgat aaattatcaa ggcttagcct attctgctgc cttgataaat
    13981 gaacctattc aagcttgcgt gattgcgcga gaagtcgatt taacagacga tataagaact
    14041 aaatttgccc aatgtttggg ttggcaagct gatcaaagtt cccctgtgtg cctcggtttt
    14101 tataaaccca ttacggtgac accgttagct agtccagatg aagtacgtat tctggcagat
    14161 acggcttcct tctatcgcac tcaaagatcc actttatccg ggcatgtaga aatgcagcag
    14221 ggccaaagag tagttaacgc tcaaacagcc tatgtgtaca gggatcctaa aacgaatgaa
    14281 gtaacaaaaa ttgaattttt gaatcatgta cgttatttag aacctgatag aatgatgatc
    14341 gctagaaaag cagttgtgta tcctcaagat aaatcgggtg aagtggaaga tgttctttat
    14401 cgatttaata ccaatagaag caatgcattg ctgcctgcct ggggtagggc aagtttaatc
    14461 aagcgttttg caaatcagga ctactttttg aaagaggcaa cttacacgac ttgcgcgcct
    14521 caagacaagg cttgggctat cgaggccgaa tccatcagta tagacaacga aaaaggaaag
    14581 ggcattgctc gcaatgccaa attacgtatc cacgaatggc cggtgcttta taccccttat
    14641 ttaagttttc ctaccaatag agatagaaaa tcggggtttc tgatgcccat agtgggttat
    14701 tccaatgtag ggggagctga tttgggcatc ccttattatt ggaatatggc gccaaattat
    14761 gacatgactc tcgttcctca cctttataca aaacgcggct tgatgttggg aggacaattt
    14821 agatatttga cttcaaagag cactggaaca tttaatggaa attttttacc aaaagataaa
    14881 gcctttggca ggtttttaca ggacaatgag gtagagttcc ctcagattcg aggactttcc
    14941 acgaatcgtt gggaagtaaa ttttgtcgat tcaacccaat ttctctctga tcttcaatta
    15001 aatgttaatt ttcaacaggt ttctgatgat tattacttgc aagatttcag tacaaatttg
    15061 gcatcggtaa ctcaaagaca attattgcgc caggctgatt taacctacac taccgaaaac
    15121 tggactttta gaggtatggg acaaagttat caaacgttac atccaattaa cgaaatcccg
    15181 gtttcccctg tttatgaacg gttaccgcaa cttatggcac gaggttatta tgatgattta
    15241 ccttttaatg cccaattgaa catccttggt caatacgacc agtttcactg gcctaatgac
    15301 tcctggaata tagcacttaa taatatgcct cagggacccc ggtttcatct gaatcccatt
    15361 ttatcagtgc caatgatgaa gccgtgggga tatgttactc cctcagtgca atttgtggaa
    15421 aattattacg atatttcaag aaattatact tggggcacat ctcgagctaa ctataacctg
    15481 acaatacctc gctatagcct tgatggcgga ttgtatttcg aacgagattt gcatcttaag
    15541 ggtacgtatt acatacaaac tttagagccc aggttattct atttaagagt accttattat
    15601 aaccaaacac ttattccagt ttatgattca gggtttatga tttttaatgt cgatcaattg
    15661 tttagaacca atcgcttttc agggtttgat cgaatcgggg atgccaatca gctaagttat
    15721 gcgcttacca caaggtggtt agaagatgaa tcaggtgctg aaaaagcgaa tttttctatt
    15781 ggccaaatca aatatttcag tgagagaaga gtacaacttt gtcaaagccc taccgggttt
    15841 tgtaccgata atccagatac ctttggcaac ttatcttcta cttttgggac atcgcctgtt
    15901 gcgtcaaggg cggtatataa atttaatcca gcttggggaa taacagggga ttacatttgg
    15961 gatccggcaa caagggctac aaataatgca gatttgaatt tgcactatca gccagctcgt
    16021 aatgctatca tcaatggcgg gtatagttat ttggttaatg gtgacgttac tcaagtaaga
    16081 aataatgaca ctgaaaataa tgccttacat caagccattt tatccgctgc ctggccgctt
    16141 agtgaaaaat ggagtggtat tggagcctat agttataata tcagtaaaaa ttacagtatg
    16201 atgtcatttc ttggcgttca atatgatagt tgttgctggg ctatgcgtat tttgggaggg
    16261 cgaactttta gaagtttaaa tgaagaattt gagcctcagt acaataacaa catttatttg
    16321 caaatattac taaaaggctt aggatctgta gctagcagtg atccaagtgg tatattgaat
    16381 acctatatcc caggatacta tgaccctttc cgacgccgtt aaaatccaaa gataaatgac
    16441 gcaataaaag cgagtcaggt ttaggtgttt aggttagtac aggttgaaaa tattttaatc
    16501 tgttttttat aaattatggt gtaaattaag caaacttgct tgtattttga caaagaataa
    16561 cataaattgc ccgaaaaatg tgaatgagga ttatggcatg tttaaaagaa tagcattggt
    16621 atgcgctttg ttttcaggag tatgtttcgc tgaaggaaag cagctgttag ataaagtggt
    16681 tgctatagta aatgataatg tcattacttc cagcgaattg aatgctcaag tcgagttatc
    16741 taaaaagcag attatagctc aaaatatgca aatgccagat gagtctgtat tacgtaagca
    16801 agtattgcaa catttaatag acgtagatct ggaaatgcaa atggcaaaac aaaatggtat
    16861 tactattgaa aatgctgaaa ttgatgaagc gatagaaaaa atagctgctt caaatcatct
    16921 aaatttatcg caaatgcgcg atgaaattac aaagcaagga attagctggc aagaatacag
    16981 gcaaaatatc cgtaaagaaa tgctgatttc cagggttcag caaaaagcag taggtaaaga
    17041 tatcattgtt actaatgagc aggtggaaca atacttaaaa actgcaggcc gcatagaaaa
    17101 ttctaacttg acgtatcatt taaaaaatat tgttatccct ttaagcgaag aaccaacgac
    17161 caaacagctt caaagagcta aaatcgaagc agaaaacttg ttaaataaaa ttaaaaaagg
    17221 tgaagacttt agccgtttgg caatagagga atcaagtgga gagtttgcac tggaaggtgg
    17281 tgatttgggt gagcgtcatt tggctgagct gcctgaggta tttgccaagg aagtagtaca
    17341 tatgaaagta ggtcaggtgg ccggcccgat aagagcagga aatggatttc atttgatcaa
    17401 attagtagct gttggcggcg agaatcagcg tcatgtgatc actcaaaccc atgtcagaca
    17461 tatactgctt aaacccgatg ccagcatggt gccttctgaa gcaattaagc aagtcaataa
    17521 catttaccgg caaatacagt caggcaaaga ttttgctctt atggctaaac aatattcact
    17581 cgatgctgcc agtgcagtga aaggcggtga tttaggatgg gttaatcctg gtgaattagt
    17641 tccagagttt gaaaaaacca tgaacagttt gccattacat aaagtcagta aaccggtaaa
    17701 aactcagtac gggtggcatc tcattgaagt gattgcacgc agacagaagg atgattcaga
    17761 agcttttaag aagcaacagg tcaggcaatt tctacaacag cgcaagtttg tagaggcagt
    17821 gcaaaactgg caacaacatt tgcgctctca agcttatatt aatatagttg ataaggattt
    17881 agcatgaacc cactgcttat tagtagtgga gagcctgctg gaattggccc ggacttatgc
    17941 ctggctttgg cagaaaccga tttgcccgta gtgatattgg gtgatctatc tttattagag
    18001 gcaagagcca gtgagttaaa tctcagtatt aaatttttag agtacagccc tcatcaaagc
    18061 ttcgaaaaaa aggcaggata tttaactgta tggcctgttc catgtgctgc accggtcatt
    18121 agtggtgaat taaatccacg aaatgcggct tatgttatgg agcttttgac tttaggggct
    18181 tcgctatgct ccaaagggga gttttctgca ttagtgacag cgcctgttca taaggcaaac
    18241 attaatgcag ccggtatcac gtttactggc cacacggagt tttttgctga tttctttgag
    18301 gtggaaacag tggtgatgat gctggcctgt tctcaaatga aagtggcatt agtcacaacg
    18361 catcttcctc tgcgtatggt gcctgatgcc attagctctt tgcttatcgt aaaggtaatt
    18421 caacaattac atcattcatt aaaacatgat tttggaattc aaagtccaaa aatcaacgtg
    18481 gctggtctga atccgcatgc tggagaatca ggatatttag gtcgagaaga aattgaaatt
    18541 attactccag ccttaaatac tctaaaaaac caggggatcg atgtgcaagg gccgttacca
    18601 gcagatacga tgtttataac aaaccatatc aatcactgtg atgcttatgt ggcaatgtac
    18661 catgatcaag gactgccagt tttaaagtat gctggattta atgaagcagt aaatataact
    18721 ctcggattgc cgataattcg tacttctgta gatcatggaa cagccttaga attagctggt
    18781 aagaacaaag ctaaccctgg gagtatgctt gcggcggtta agatggcgaa agatatggct
    18841 ttaacaagga taaaatgaaa tgtctctgat tagcctgata gcagccatag atgaaacagg
    18901 aggattgggt attaacaacc aattactctg ctatttgcca gctgacttac agcactttaa
    18961 atcgattacc atgggtaagc ctattatcat gggcagaaaa acttatgaat cgataggcaa
    19021 acctcttcca ggcagactaa atgtcgtgat tagtcaatcg attcagtcaa taccgggtgt
    19081 tgttgttgtc gattctttgg gccaggccat aaaagaaacc gctgacgcat cagaggtaat
    19141 gatcattgga ggagccaaga tctattctca agccatatca atggctaatc gcttatatat
    19201 tactcgaata caccatcaat ttacggccga tgtatttttt ccaaaaatca atgaattggc
    19261 atggtgctgt cgaagcaagg agtatcgaca gcatgatgac aaaaatcaat acgatatgac
    19321 gttttgcgta tatgaaagaa tatcaatttg atagctatca ataatgataa tgtctgtttt
    19381 gtgagggatg tctgtgcact ggtggctgag tccttacaat tatttcagtt ggtctgctca
    19441 gtattctata attgtccgtt ataatttccc gaccaccata agaaatatta caatgtgagt
    19501 aaccaggcca tgttttccct ggctgaatac ttccattgaa gttactttga cagacaaata
    19561 aaggattacc attggtatct ttacccatta acaatgcatg ctctacattg ggttcccagc
    19621 tgtatcgccc aaattcattt tgattcggta tggtaaactg gctaagaacg tactctttac
    19681 caccataagg tacgttgcat cgaccatatc ctgcccatgt ttttccgggc tgtatactgt
    19741 taaacagttt tgccttacac agatataatg cattgccatt cgtgtcagtt ccagttcgta
    19801 acgcattagc taacggtgaa acaggatgtt gatggtaatg agtattggcc caggcaggat
    19861 taagaaagaa tatcatcaat aaaaggtata caagtttgga catatcaact ctctaaagat
    19921 ttaaagagcc gcaagcataa cgcaagaaaa aaatatgcgc aagatgaata cttaaagatc
    19981 gatatggttt atctattgta acctaaagaa aaacaattaa aaaaaattaa aaaagtattt
    20041 gacagggttg ttgaaatgag tagaatacgc cctacgccac gagggcgggt tcattaagaa
    20101 gagagaagac aaactgtgtg ggcactttga gatactttga gtgcttaaga gataaagaag
    20161 atagctagcg agagctagtg ccggaattga actgaagagt ttgatcctgg ctcagattga
    20221 acgctggcgg catgcttaac acatgcaagt cgaacggcag cattgtctag cttgctagac
    20281 agatggcgag tggcgaacgg gtgagtaacg cgtaggaata tgccttgaag agggggacaa
    20341 cttggggaaa ctcaagctaa taccgcataa tgtctgagga cgaaagctgg ggaccttcgg
    20401 gcctggcgct ttaagattag cctgcgtccg attagctagt tggtggggta agggcctacc
    20461 aaggcgacga tcggtagctg gtctgagagg atgaccagcc acactggaac tgagacacgg
    20521 tccagactcc tacgggaggc agcagtgggg aatattggac aatgggggca accctgatcc
    20581 agcaatgccg cgtgtgtgaa gaaggcctga gggttgtaaa gcactttcag tggggaggag
    20641 ggttgatagg ttaagagctg attaactgga cgttacccac agaagaagca ccggctaact
    20701 ccgtgccagc agccgcggta atacggaggg tgcaagcgtt aatcggaatt actgggcgta
    20761 aagggtgcgt aggtggttga ttaagttatc tgtgaaattc ctgggcttaa cctgggacgg
    20821 tcagataata ctggttgact cgagtatggg agagggtagt ggaatttccg gtgtagcggt
    20881 gaaatgcgta gagatcggaa ggaacaccag tggcgaaggc ggctacctgg cctaatactg
    20941 acactgaggc acgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgctg
    21001 taaacgatgt caactagctg ttggttatat gaaaataatt agtggcgcag caaacgcgat
    21061 aagttgaccg cctggggagt acggtcgcaa gattaaaact caaaggaatt gacgggggcc
    21121 cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg cgaagaacct tacctaccct
    21181 tgacatacag tgaattttgc agagatgcat tagtgccttc gggaacactg atacaggtgc
    21241 tgcatggctg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgta acgagcgcaa
    21301 cccttatcct tagttgccag catgtgatgg tggggactct aaggagactg ccggtgacaa
    21361 accggaggaa ggcggggatg acgtcaagtc atcatggccc ttacgggtag ggctacacac
    21421 gtgctacaat ggccgataca gagggcggcg aaggggcgac ctggagcaaa tccttaaaag
    21481 tcggtcgtag tccggattgg agtctgcaac tcgactccat gaagtcggaa tcgctagtaa
    21541 tcgcgaatca gcatgtcgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca
    21601 ccatgggagt gggttgcacc agaagtagat agtctaacct tcggggggac gtttaccacg
    21661 gtgtggttca tgactggggt gaagtcgtaa caaggtagcc gtaggggaac ctgcg
//
LOCUS       DBNCWK010000041        21591 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.041, whole genome shotgun sequence.
ACCESSION   DBNCWK010000041 DBNCWK010000000
VERSION     DBNCWK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21591
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.041"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            133..1086
                     /gene="ceg5"
                     /locus_tag="AC06IO_001378"
     CDS             133..1086
                     /gene="ceg5"
                     /locus_tag="AC06IO_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg5"
                     /protein_id="HIO9874577.1"
                     /translation="MISQKEALALLNKYEKHLNELQNIITTSYQNLLRCDAQIIPNAL
                     AALKAIANNTPFVSVVATSGMSSIKALSSDPQIALKELLVNDFFKDHFQKMCIHYAKD
                     KPELLTNLQNFHACVSQEKIIIYKAKNGELALKFPTQEWRDRFIQKMGGVSHFASSDK
                     FNANKKCPQTYPENPTTLYLSTYTAKNGELAVVFPNKEIRQNFLTLLRHASSATWSIY
                     EGQDAVYFNDKNLHTPGYNFGINAPACVSQSSNAAHADVRYAARILGQALRNPNNTCF
                     FNKLPAELVNKIISLSTDNEVLAEEEAINKVQSYLGKPQLK"
     gene            complement(1293..1862)
                     /locus_tag="AC06IO_001379"
     CDS             complement(1293..1862)
                     /locus_tag="AC06IO_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIO9874578.1"
                     /translation="MKNTLLPNLIVIALAATISANFMTVQAKQNDKIQNSNEQLIQDP
                     FGTDPFFQSHDDVLQQMHKMQQAMDQFIKSQFSQMQNSLVNQPGQNPFGSAKNIQITE
                     NKDELIYKIKLPQGADSKVDVSVKNGQLVISANVTQKITREQDNNKSVSFSQSNYMQS
                     FPLPKGYDPNSMVTKMKDSNLIVTFKKHS"
     gene            complement(2364..4613)
                     /gene="katG"
                     /locus_tag="AC06IO_001380"
     CDS             complement(2364..4613)
                     /gene="katG"
                     /locus_tag="AC06IO_001380"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HIO9874579.1"
                     /translation="MFKRTIPLFAAFTLAISPSIFPNYAHAQEDKPKTNQYWWPKMLD
                     LSPLRQPNATSNPMGEKFNYAEEFNSLDLNAVIEDLKKLMTTSQDWWPADYGNYGPLF
                     IRMSWHAAGTYRIYDGRGGANGGFQRFAPQNSWPDNANLDKARRLLWPIKQKYGRKIS
                     WADLLVLAGNVAMESMGFKTIGFAGGREDAWEAININWGPEGKWLESKRQDKDGKLEK
                     PLAATVMGLIYVNPEGPNGVPDPLAAAEKIRETFGRMAMNDEETVALIAGGHAFGKTH
                     GAASSKYLGPAPEAAGIEEQGFGWKNSYGSGKGKDTITSGLEGAWTVTPTHWSHNYLQ
                     NLFNFNWVKTKSPGGAIQWVPENSNASSMVPDAFDPSKRHAPVMLTTDLALKFDPVYS
                     KIAKRFLDNPKEFDDAFARAWFKLIHRDMGPRSRYLGSLVPKEIMIWQDPVPPVDYKL
                     VDANDIANLKDKILNSGLTTPELVKTAWASASTFRGTDMRGGANGARIRLAPQKDWPA
                     NDPQELAKVLKTLESIQNNFNNAQTDGKKISLADLIVLGGNAAIEHAAKQAGYDIIVP
                     FTPGRTDATQEMTDVKSFEVLEPKADGFRNYFDKSNNMSPPEMLVEKSSLLKLNVPEM
                     TVLVGGMRVLNANTGQKQYGVFTDKPGTLNNDFFINLLSMSTEWKKSSETEGIYEGYD
                     RKTGKLKWKATSVDLIFGANSELRAVAEAYATDDAKDKFIQDFVNAWVKVMTADRFDI
                     KAAKANINS"
     gene            complement(4745..5749)
                     /gene="ravE"
                     /locus_tag="AC06IO_001381"
     CDS             complement(4745..5749)
                     /gene="ravE"
                     /locus_tag="AC06IO_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavE"
                     /protein_id="HIO9874580.1"
                     /translation="MENDMPKTKYALPPVVLFESHADRSTSDFLIKQLPDLKKAGYTT
                     ICVDGMEPGASLEENISMMKILIQIQVKKLSELPLEHPEYEQGVEKLRSVVAKLDLFE
                     AMKEQGFKLGGIDLPVSEQLKEKSLNSIRREQTITDNTLKHVKENDGGVVVVLGFGHC
                     IFQQMIKEQDENANQYLWYHVHNPDNETQAYKELIESYTKKGLSTYFPLGVNIFKSSD
                     KELDTDFWNKVSANCYNYDPKALETSTASILKSLVGPEVTAHLRTDGQHHVDALISLE
                     TVEKTHQIKSSDFLRSLSKTLGNIHFEVAKIKTKDQVIIRGINEPEVAEQISKLSKKM
                     "
     gene            complement(5951..7237)
                     /gene="ravF"
                     /locus_tag="AC06IO_001382"
     CDS             complement(5951..7237)
                     /gene="ravF"
                     /locus_tag="AC06IO_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavF"
                     /protein_id="HIO9874581.1"
                     /translation="MKSFWLCGDFIMSLEIYTWKNGSKGLGFTKPVKSMLFGGNVGHA
                     AVELTFPADAKGDELAKKYQDVPGLSISKRTEIVPEKQEDGSYKPKEQVVYFVYFSWW
                     PGHTNGHHINSHREDLESEWRHEPAPKIKPEIQEHLYGENTPAANKTNVTGILISDKT
                     ITKVKEISHQSIKETTTIENDPAYQQLKAEEAALQTELQQLIDKRKTYEQELARSGLE
                     QRQPNKELCLTDIEIARGYEISKELKRVAEQIKLCQMDFEERYLSVGEPPSSVIRIPT
                     TLDHNAPTFALDAAGILDKMASLAQSTTAYSFYKFNCSTSATQVVKAGISDELKNIMK
                     TDGFNVEKASQTTIATPTSFRHFSQQVQTELILLNTNQALVEQQRAKTENTSEAPTTS
                     TQSFKQKLQDAMKGKSEEAVREQDESEESRIGLTIS"
     gene            7596..7913
                     /locus_tag="AC06IO_001383"
     CDS             7596..7913
                     /locus_tag="AC06IO_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HIO9874582.1"
                     /translation="MTMMITTGNSFEGKVIKQYLGIVRGIVVRSPTISQGLMGGLKSI
                     VGGKIGAYSQMCEHAREEAFQLMIEHAQALNANGIIAMRYDTGEIGQAGTEVLCYGTA
                     VII"
     gene            8111..9481
                     /locus_tag="AC06IO_001384"
     CDS             8111..9481
                     /locus_tag="AC06IO_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIO9874583.1"
                     /translation="MLVELLSRIQFGFSIGFHILFPTLNLGLAVFLVIMEALWLKTKN
                     PVYLEICKFWTKIFALTFGMGVVSGIVLAYQIGTNFGPFITQFGNVLGALFAYETLTA
                     FFLEAGFLGVMLFGWKRVPPALHFIATLLVTIGTTVSAFWIMSANSWMQAPSGYQLIE
                     GKYVVDSWWTVVFNPTFIPRFIHMLLASYVTTCFVIMAVSGFYFLRKQSTAIAKKSLS
                     FSLWASLILVPLQIGVGDVVGINVHHYQPLKTAAMEGIWETQKGAPLLLFAIPSQTQQ
                     KNFYEVGIPKLASLINTHEWNGEMIGLSSVGNEEQPRMAPVFFSFRIMVGIGLIMLFT
                     AIAGFILRLRGTLFENSWFHRWCIVMAPLGFIASIAGWLTAELGRQPWVVHGLLKTRD
                     SVSAIGVEEVIISFVLLIVAYGVVFGFYLYYLLRLISHGPRILDEDEAEHHAFQYMTD
                     FPKEKK"
     gene            9481..10470
                     /gene="cydB"
                     /locus_tag="AC06IO_001385"
     CDS             9481..10470
                     /gene="cydB"
                     /locus_tag="AC06IO_001385"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIO9874584.1"
                     /translation="MLPFIFATLLGFIVLMYVILDGFDLGIGILFPFTSSESERDKMM
                     NSIAPVWDGNETWLVFGGAMLYGAFPQVYGLLLPILYMPIMLMLIALIFRGVSFEFRF
                     KAKRSKTLWNWSFSISSVAATFLQGVILGAFVQGFSINESSMTINDPDWLTPFSLCTG
                     IALVCGYALLGSTWMIIKSTGRLQRKMVHYARGLLVLVSFFLLFVSIWTPLHSEEVFH
                     RWYSFPNVLLLSPLPLIALMAICLVWQNLSSGSEYKPFIYSIIIFLCSYIGIAISVYP
                     YLIPHQVTLWEAAAPDSTLVFILIGVAIMLPILLAYTLYAYYLFRGKSSENYH"
     gene            complement(10471..11256)
                     /locus_tag="AC06IO_001386"
     CDS             complement(10471..11256)
                     /locus_tag="AC06IO_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIO9874585.1"
                     /translation="MNKMIILLVIAIIFLHMCLIWLWYRYTNNPSVVDVGWASGLTLA
                     GLIYLNNQEFSLRSLLLVLALLAWGIRLGGYLWWTRIRLKKIDKRYLALSNDWKLAKP
                     LGFFLNFQLQGVLICLVSIPWYFSSSETQTTLNLLDGLALMIFLTALILETVADNQLQ
                     DFKKNYPGKVCNQKLWRYCRHPNYFYEWLVWCSFTLFALSAPYGWIAIISPLTLYLIM
                     TKITAPMTEQGSIESRGNLYIEYQKVTPMFFPKWLKNNSTPRK"
     gene            complement(11253..11789)
                     /locus_tag="AC06IO_001387"
     CDS             complement(11253..11789)
                     /locus_tag="AC06IO_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HIO9874586.1"
                     /translation="MIINPLHIVHYLSYYLCWIFCIYLASYGYPLLGLIITVLIMGLQ
                     IIVQILNQLPWRDALYFSIGLALLGAITDAIWLYSGMIYFKANFFGSHFPPLWMVALW
                     LSFGFNLVVLYGSWLTFYFLWALVMFPSIIFAYWLGVQFGAATLIGSEVVFYLTLGLT
                     WSLFLPLSLYLFNYRHRK"
     gene            complement(11779..13566)
                     /locus_tag="AC06IO_001388"
     CDS             complement(11779..13566)
                     /locus_tag="AC06IO_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIO9874587.1"
                     /translation="MPLDKLTGITFHAAIIAGCHRLIRHKQILNAINVFPVADGDTGD
                     NLSSTCMAIIYFSSVKADLRGTLESIADASIIGARGNSGIIFSQFFNLLAKHLPLKEE
                     LYFNDFSKIMVQVGKEIAAVVTNPVQGTMITLIQKWATVCEIKQPDSLLAQAMLELLP
                     LLAMEVEQTKNSLNILQKANVVDAGALGFYYFVEGFTQFLQQSHTTDTANTHSPLSIT
                     EHPQLTCTTTEYRYCTEAVLKSASIDKNKLLHFLKEQGECASVTGNDRITRFHVHTNN
                     PREIFTSLYKDYTIQYPKVDDMQRQHELQFGKKYKIGLVTDSSADLPQDLIDEYQIHQ
                     IPLNIHVDEHHLLDKHCFEANYFYTQLNSFRTYPKTSCMNPNLIEEKLAKIASHYNHV
                     LVLSISSKMSGTYDAFLTATKKYANITVLDTKTNSGAHGLLLHYAGQLIASGNDIDTI
                     TKLLGKAIKNTYIFVMVNQFKSMIRSGRVSKISGRIAQWVNIKPIVSIDSAGQGIMVG
                     KCFSASRARNKLINFLQEKKDEAQLILEDYCIIHAGDKNQALHLAEMTTQAFNKPPTY
                     IESVSLTIGLHAGQGCIAIAGRLRECNDY"
     gene            13649..14347
                     /locus_tag="AC06IO_001389"
     CDS             13649..14347
                     /locus_tag="AC06IO_001389"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9874588.1"
                     /translation="MARYLVIAASSAIGQSVTTLLKNRGDTVFTTARDNHKISPDFQL
                     DATHFDAVLDVFRQAGPLDGVVNCAGSLLLKNAHSTSFDEFQVAINASLTTSFATIRA
                     AGLTMNQGGSVVLISSAAALVGLANHEAIAAAKAGIIGLAQSAAATYASNNLRVNVVA
                     PGMVNSPLTSSLLSNQLVLNASKTMHALGRIGVPEDIAHAIVFLLNPDNSWITGQVIA
                     VDGGLSRVRPKMKI"
     gene            14364..15902
                     /locus_tag="AC06IO_001390"
     CDS             14364..15902
                     /locus_tag="AC06IO_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIO9874589.1"
                     /translation="MTTLCFILGDQLNETIASLAAINKQKDIVFLCEVAEETSYVPHH
                     PKKIAFLFSAMRHFAQQLKKKGYRVRYTSFDDPRNQGSFAKELLRAIEEEQVSAISLT
                     EPGEWRVLKIFHDLKSQLTIPVTLHEDNRFLCKINEFRNWAKEKKQLRMEFFYRMMRQ
                     KYHLLVDNDGKPVGGSWNYDAQNRKNANHISSFPKRLEHTRDVITDEVLGLVSARFAK
                     HFGQCHPFNLAVTREQALEEADYFMEHFLCNFGAYQDAMLKDEISLYHSKLSFYLNAG
                     LLLPLELCQMAEQAYFSKQMPLNSVEGFIRQILGWREYVRGIYWLFMPDYGEMNYFNA
                     CRPLPSFFWGEETGMFCIKEVVRQTSTEAYSHHIQRLMITGNFALLAGLDPKQVCEWY
                     LAVYADAYEWVELPNTLGMALYADGGLMASKPYAASGKYIHRMSNFCQSCYYNPNDLL
                     GEKACPFNALYWNFLHQNQEKLKDNPRLRYAYMNWQKMDCDKQKKTIHKAQRILADLD
                     SANL"
     gene            complement(15907..16320)
                     /locus_tag="AC06IO_001391"
     CDS             complement(15907..16320)
                     /locus_tag="AC06IO_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HIO9874590.1"
                     /translation="MNGWTLKVFAIAFLIFIVTDMIWLGFIARNLYFDQYQQWLRLSE
                     GQLKPIWWSALLVYLLFALSIVVFVIPLAKTSLPYAALYGALLGAIIYGIYDFTCLAI
                     FKDFPVGMAFLDWLWGIVLCSWSSIATIYVASYIK"
     gene            16688..19573
                     /gene="legK4"
                     /locus_tag="AC06IO_001392"
     CDS             16688..19573
                     /gene="legK4"
                     /locus_tag="AC06IO_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector kinase LegK4"
                     /protein_id="HIO9874591.1"
                     /translation="MKLLRFHELKSLPGMDEKALELLIKVLGNKGIRKLIKSADGKPI
                     SREIMIHEFGIDCQILFITTEASLKPIIVPTENKISGGGKSYCEQFKVYALDDGKTYF
                     LKSVKIDAESLTEFTNEKDTLSKLGRLVGTFFNEQTQVHYILTTFIKGIDLSRYKNAL
                     PLNVNLKHFWEVLGIMISVCHQVKQFHELGLIHRDLKPGNIMLDADMQCHLVDFGSSS
                     SDKEPKPASWGTACYLAPELKANEDFIAFSQASDLFALAYSLNELFNPFRQVKFAKVD
                     IGIKNKHLALLHAEIEACITGLMSNETLVRTLYFSRILQLQRVPESFKSKPEAYTYMI
                     MLLTQWKSCYEAPEMNKELDEIIAEIKVAYENHEQDAVKIITLLEQLSKADGLLNSHK
                     ALLSVLIKSLANVQQQELGHDDILPRRFESDVVSHLIKTPTAKMMAAIKQVSDAIVKI
                     LEQYEHSPTESFEHEVLQDFLEQMVQSDILFGALKEKIEISDVIAILKANRPEDFVQI
                     QHISFKFAQVALRDLALHDLPKHEPKGAFLEVLKKYQAKYAPEETSLFELYHHMAGVR
                     DHNPSKVKKGEGYRFRAFICDELFYGDEIFTTGDNRGREGKPNQRLQTNQVGLMKFEH
                     SAHTKGLLTLNGQSWYADCKTQLPNYDSIHYLSALKTDCPYITGPSGMTSLFMNMMFL
                     LLNPKDQDVILSYSLGVMTYVVGAGYHSIKEILIPMVKCVGIVPDYPQHKGTECLTAP
                     PLYNHYFKAIEEFDKEFAEAHEKIWQEYLAYFRMTYMPVCMRSHCLPHQVPSIEDISS
                     EVKEMLEIVSKSVKNCLEEHKVKRTGDMGLFLSTPCGQTKALEVLSHVCQQQVGLTAI
                     FRQIQAYFKGTFETEEGIIINREMQLKFIAHFFDVLKESPTLLSQFNLTLRIENTIGV
                     HQCELGSSKQKELFLEIKKTSIAANEEQMVANHEPRKENRNVIILPY"
     gene            19592..21562
                     /locus_tag="AC06IO_001393"
     CDS             19592..21562
                     /locus_tag="AC06IO_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874592.1"
                     /translation="MLSKEKINPSLKNTKRQGSDNLAAMADPASLKNTGDYRCFNSKP
                     EAYTYFVFLLNQWQTNYMDDKREAIAKAIDSIKMAYENHEQDTLVVHKMLHGLSHLLE
                     QEHADELNALCLVPIKDSQSNANNILPRRFESELACRLIESPTKSMMQAIDLVSQAII
                     NLIEEYSKLDSKSPERATLNFFLQELEGSDIEFGAFKKRPSLEEVIAILKENNPKDFI
                     QIQFISFMFARSVIGPFPLRNLPPFKPTGLFAQVLDKYLQVHPDLKEEEEGFSYQLMA
                     AKNEAEEFRTFITKKLFYQDELYTQGENRGRQGPRKTYLKTNQLGLMKLDHSAHSVGL
                     TVIPEQIWCADAQAQEANQNSIYYKTALTNDCPYVTGPSGMTSIYMNMMLLLLNPQNI
                     ELIQAYSLGIIAYIVGAGFHSVNEILIPLVKCINLLPGYPNYEGLEYLTHPPLYHEYF
                     KQIAQFDSDFIQLRDQVWAYYLNYFKSCYMPLCMRDFCEAEQLANCPDSAPEVKILCG
                     IVLKSIEDCINFHNLNRHLDSRFLGTTTADIDALNQLKEACSSQSSISGIMRLLQKYF
                     SGENIARPTAMHKFETRSYIRFFLDALKDNPQIISHINEKMKLDPPLVISTKAEIFSD
                     DFRIECFNVHRILKREHQVSENCNNTSQFLKL"
BASE COUNT         6304 a         4096 c         4382 g         6809 t
ORIGIN      
        1 ggcaatacca agaagcaagt gaatgtaatg gtttataaaa agaacacaat gtcttgggtc
       61 aaaattaatt taatattgat ttaataattt actgttagaa tttcgagcca tgtgaaaacc
      121 tgaggaaaaa atatgatatc ccaaaaagaa gcacttgctt tactcaacaa gtatgaaaaa
      181 cacttaaatg agctgcaaaa tattattaca acaagttatc aaaatctttt acgttgtgat
      241 gcgcaaatta tacccaatgc tctggctgct ttaaaagcga tcgcaaacaa tactccattt
      301 gtatcagttg tagctacttc aggcatgagc agtataaaag cgttgagttc tgatccacaa
      361 atagctctta aggaattgtt agtgaatgat ttttttaaag atcatttcca aaaaatgtgt
      421 attcactatg ccaaggataa accggaatta ttaacaaatt tacaaaattt tcatgcatgc
      481 gtaagccaag aaaaaattat tatttataaa gcaaaaaatg gagaacttgc actaaagttc
      541 cctacccaag aatggagaga ccgatttatt caaaaaatgg ggggcgtatc tcattttgca
      601 tcttctgata aattcaatgc caataaaaaa tgtccgcaaa cttatcctga aaatcccact
      661 acgctgtatc tcagtaccta tactgcaaaa aatggagagt tagccgtagt ttttcctaat
      721 aaggaaatac gtcaaaactt tttaacttta cttagacacg cctcttcagc aacatggtca
      781 atttatgaag ggcaggatgc ggtatatttt aatgataaaa atttgcatac ccctggatat
      841 aactttggca ttaatgcacc tgcgtgtgtt tcacaatcat ctaatgctgc ccatgcagat
      901 gttcgttatg ctgcaaggat tttaggccaa gctcttagaa atccaaataa tacttgtttc
      961 ttcaacaagc ttcctgctga attagttaat aaaattataa gcttaagcac agacaatgaa
     1021 gtgctcgccg aagaagaggc cataaataag gttcaatctt acttaggtaa gccgcagtta
     1081 aaataatcag ctatttctaa aaatggtgcc ggggcttgaa ttttatgtgc aaagacaggg
     1141 aatctttgca atagaaaatt caagtcctga ctcttgattt ctttttttaa cggatgcttg
     1201 aagaaatgaa agtttcaagc ttgtatcaaa ctatgggagc aatcttgccc atagttgttt
     1261 tagtaattta tttaatcaag cagacaatgc ggttaagaat gttttttaaa agtaacaatt
     1321 aaatttgaat ctttcatttt tgtaaccatg gaatttgggt cataaccctt gggcaatgga
     1381 aatgattgca tgtaattgct ttgagagaag ctgacactct tattattatc ttgttcacgg
     1441 gtgattttct gtgtgacgtt ggcactaata actaactgtc catttttaac agaaacatcc
     1501 actttgctat cagcaccttg cggtagtttt atcttataaa taagctcatc tttattctct
     1561 gtgatttgaa tgttttttgc actgccaaat ggattttgac caggttggtt tacaaggcta
     1621 ttttgcattt gtgaaaattg actcttgata aattgatcca tagcttgttg cattttgtgc
     1681 atttgctgca gaacatcatc atgtgattga aaaaaaggat cagtaccaaa tggatcctgg
     1741 attaattgtt cgttgctatt ttgtatttta tcattttgtt tcgcctgaac ggtcatgaag
     1801 ttcgcagata tcgtagcggc aagagcgatg actattaaat tgggaagtag tgtatttttc
     1861 attgaaatct cctcacaaga tttgtttata aaaaattcta tctccattta atatggatat
     1921 tcttacttca atccacttat tagacctctt gtataaactg actctttcgt tttattgctg
     1981 cgttacaaac tttctgcgtg ctcatttact atatacaaac tcttctcctc aaaacgttag
     2041 cgccttggcc taaaacaaaa taatcagatg atgtaagaga tctattttaa agcggttatc
     2101 tcccattggt gaatatgaaa attacaagca agatattgcg gttaaaccag attttttcaa
     2161 gtacaaattg atgaatattt ggagcgggac aaagccatat aaagccatat aacgtatagt
     2221 tcaagagagg gcatagatca gtatggaaaa ccatagaaat ttagctaaat tagccgggtt
     2281 ttgttaaacc attgatttga gatttggggt aacaaacgat gatttagtca cagtttgtca
     2341 ccctaaatct gaaggaatga agattaactg ttaatgttag cttttgccgc tttgatgtcg
     2401 aagcgatcgg ctgtcattac cttaacccag gcattaacaa aatcttgtat aaatttatct
     2461 tttgcgtcat cggttgcata ggcttctgca actgctctta actcagaatt tgcgccgaag
     2521 atgagatcaa ctgaggtcgc tttccatttc agctttccag ttttgcgatc ataaccttca
     2581 taaatccctt ctgtctcact tgatttcttc cactctgttg acatacttag cagattgatg
     2641 aaaaaatcat tatttagagt tccaggtttg tcagtgaata cgccatattg tttttgtcca
     2701 gtattcgcgt taagtacacg catcccacca actaaaaccg tcatttccgg aacatttaat
     2761 ttcaacagac tcgatttttc caccaacatt tctggtggcg acatgttgtt gcttttatca
     2821 aagtaatttc taaacccgtc tgctttaggt tcaagaacct caaaagactt cacatcagtc
     2881 atctcttgtg tagcatccgt acgtcctggt gtgaagggaa caataatgtc gtagccagct
     2941 tgcttggccg catgttcaat tgcggcatta ccacctaaaa ctatcaaatc cgcaagagat
     3001 atttttttcc catcggtttg agcattattg aagttattct gaatgctctc caatgttttt
     3061 aatacttttg ccaactcctg aggatcattt gccggccagt ctttctgggg agccaggcga
     3121 attcgcgcac catttgcccc accacgcata tcagtgccac ggaaggtgga tgccgaggcc
     3181 caagcagttt tgaccagctc gggtgtggtt aatcccgagt ttaggatttt atcctttaga
     3241 ttggcaatat cattggcatc gactagtttg tagtcaaccg gcggaacagg atcttgccaa
     3301 atcatgattt ctttaggcac taaagatccg aggtaacgtg aacgtggccc catgtctcgg
     3361 tgaatcagct taaaccaggc acgagcaaat gcgtcatcga actccttagg attatcaaga
     3421 aaacgcttgg caattttact gtaaacggga tcaaatttta gtgccaagtc tgtggtcaac
     3481 atgaccggcg catgcctttt actagggtca aatgcatcgg gaaccattga cgaggcattc
     3541 gaattctcag gcacccactg gattgctcca ccggggctct tggttttaac ccaattgaaa
     3601 ttgaacaggt tttgtaagta gttatggctc cagtgggtag gtgttacggt ccaggcacct
     3661 tctaaaccac tggtaatcgt atctttccct ttgccgctgc cgtaactgtt tttccaacca
     3721 aagccttgtt cttctatgcc cgctgcttct ggcgctggcc ccagatattt gctggacgcg
     3781 gctccatgtg tttttccgaa tgcgtgcccg ccagcaatta aagcaactgt ttcttcgtca
     3841 ttcatggcca tacgcccgaa agtctcgcga attttttcgg ccgcagcaag aggatctgga
     3901 acgccgtttg gtccttccgg attcacatag attaagccca ttacggtcgc agcaagcggt
     3961 ttttcgagtt ttccatcttt atcctggcgc ttactttcca gccatttgcc ttcagggccc
     4021 caattgatat tgatggcctc ccatgcgtct tctcgccctc cagcaaatcc aatggtcttg
     4081 aagcccatag attccatcgc aacatttcca gcaagtacca acaaatcagc ccatgaaatt
     4141 ttgcgcccat atttctgctt aattggccac agtaaacgtc tggctttgtc cagattggca
     4201 ttatccggcc agctgttttg cggggcgaaa cgctggaagc caccatttgc tccaccacgg
     4261 ccatcataga tgcggtaagt tcctgccgca tgccatgaca tgcgtatgaa taatggacca
     4321 tagttaccat aatcagcagg ccaccagtct tgcgaggtag tcattaattt tttgagatct
     4381 tctatcacgg cattcaaatc caagctattg aattcttcgg catagttgaa tttttcaccc
     4441 ataggattcg aagtggcatt gggttgccgc agcgggctaa gatcgagcat tttaggccac
     4501 cagtattgat tggttttcgg cttgtcttct tgcgcatggg cataatttgg aaaaatggaa
     4561 ggcgagatgg ccagagtgaa tgctgcaaac agaggtattg ttcgtttgaa catagaaatg
     4621 actccctttt ctatacagaa aaggcaaagt atactccatg ttacttttaa gcagcaacaa
     4681 tactttaagc agttaattta attaaggtaa tctgaaaata tattttaatt tcagaatatt
     4741 aggtttacat tttttttgat agcttgctta tctgctctgc tacctccggt tcattaatgc
     4801 cacggataat tacctgatcc tttgtcttga ttttggctac ttcaaagtga atgttaccta
     4861 aggttttgct taaacttctc aagaaatccg aagattttat ttgatgtgtt ttttcaactg
     4921 tttcaagaga aattagtgcg tccacatgat gctgcccatc ggtgcgcaag tgcgctgtca
     4981 cttccggacc gactaaggat ttcaaaatgg aggcagtcga tgtttcaaga gcttttggat
     5041 cataattata gcaattggcg cttaccttat tccaaaaatc ggtatccaat tctttatctg
     5101 agcttttaaa aatattaact cccagtggga aataagtaga gagtcctttt tttgtgtagg
     5161 attcgatcaa ttctttgtag gcctgtgttt cattatccgg gttatggaca tggtaccaga
     5221 ggtattgatt ggcattttca tcttgctctt taatcatttg ctggaatatg caatggccaa
     5281 atcccaggac aacgactact cctccatcat tttctttcac atgctttaag gtattatcgg
     5341 taattgtttg ttctcttctt atggaattga gactcttctc tttaagctgt tctgaaaccg
     5401 gcaagtcaat tcctccaagt ttgaatcctt gctccttcat tgcttcaaaa aggtctaatt
     5461 tagcaacaac gcttctcaat ttttcaacac cctgctcata ttcaggatgt tcaaggggta
     5521 gctcagataa tttcttgact tgtatctgga tgagtatttt catcatagat atattttcct
     5581 caagggatgc gcctggttcc attccatcaa cacaaatggt tgtatatccg gctttcttca
     5641 aatctggaag ttgtttgata agaaaatcgc tcgttgacct atccgcatgg ctctcaaata
     5701 acacaactgg aggaagggcg tatttggttt taggcatatc attctccatt tgagatatat
     5761 ctttaaatta tagttgatta aatttaatcg ctcatttaat gagcaacatg ttggagtgtg
     5821 gctatctaag gaataatttt tcagattatt ttacttgcca gtatgtgttg tagttatcgg
     5881 ttaagtttat cttattcatt attgttatgc tggaactcaa ccgattaatt cttttggaaa
     5941 gatggaaata ttaacttatg gttaaaccta tcctgctttc ttcagactca tcttgttctc
     6001 taactgcttc ttccgatttt cctttcattg catcttgaag tttttgtttg aagctctgtg
     6061 tgcttgtggt tggtgcttca gatgtattct ctgtttttgc tctttgctgt tccacaagtg
     6121 cttgattcgt attaagaaga atcaattccg tttgaacttg ttgactgaaa tgacgaaacg
     6181 atgtgggtgt agcaatggtc gtttgagatg ctttttctac attaaaccca tcggttttca
     6241 tgatattttt tagctcgtca ctgataccag ctttcaccac ttgcgttgct gatgtggaac
     6301 aattaaattt atagaagcta taggcggttg tgctttgtgc caaactagcc attttgtcaa
     6361 gaattcctgc tgcatcaaga gcaaaagttg gagcattgtg atcgagtgtt gtgggtattc
     6421 taatcacgga tgaaggaggc tctccaacac tgagataacg ctcttcaaag tccatttggc
     6481 ataacttgat ttgctccgcc actcttttta gttctttgct aatctcataa ccacgagcga
     6541 tttcaatatc agttaaacag agttctttat taggttgtct ctgttcaagg ccagatctgg
     6601 ccaattcttg ttcataagtt ttgcgtttat caattaattg ttgtaattca gtttgtaaag
     6661 ctgcttcctc tgcttttaat tgctgatagg ctgggtcatt ttcaattgta gtagtttctt
     6721 taatactttg atgggaaatc tcttttactt tggtaattgt tttatcacta attaatattc
     6781 ccgtgacatt ggttttatta gcggcaggag tgttttctcc atacaaatgc tcctgtattt
     6841 ccggtttaat tttaggagct ggttcatggc gccattctga ttccaaatct tctctgtggc
     6901 tgttaatgtg gtgaccattg gtatgtcctg gccaccagct aaaataaaca aaataaacaa
     6961 cttgttcttt aggtttataa cttccatctt cttgtttttc tggaacgatt tctgtgcgtt
     7021 tactgattga aagaccgggt acatcctgat attttttcgc taactcatct ccttttgcat
     7081 cagccgggaa agttaattca acggcagcat ggccaacatt gccaccaaat aacatggact
     7141 tgactggttt ggtaaacccc aaccctttgg aaccattttt ccaggtatat atttcaaggc
     7201 tcataataaa atctccacac aaccagaatg atttcattat gacccaattg attaaaaatt
     7261 aaattaaagg gggcttatat gtgcagggaa tctcagcatc atacattcct aacaggaata
     7321 acggtgaatc tttaaagatt catcaagact tatgaaaaac gtcaatagga gcgaaaacct
     7381 gatctgataa gtgggaatat ttctcagtat aaacaaaatg gattaactat tcttgccaat
     7441 gctaatctgt aatccggcaa atgaattgta tttataaata aggggggctt tatttagaat
     7501 tctctcgtag ttttaataat ttttttcaat aagcttctat tttcaagatt ttaaactaat
     7561 atggaaagat aaaagggata tttagtgagc agattatgac aatgatgatt acaacaggaa
     7621 attcgtttga agggaaagta attaaacaat atttgggtat tgtgagaggt atagtagtgc
     7681 gctctcctac catcagccag ggattgatgg gaggcttgaa atcgatcgtc ggaggaaaaa
     7741 ttggtgcgta ctcgcaaatg tgtgagcatg caagagaaga agcatttcag ctcatgatag
     7801 aacatgccca ggcactcaat gccaatggca ttattgccat gcgttacgat acgggcgaaa
     7861 ttggtcaggc cggcacagaa gttttatgtt acggaacggc tgtgattatt tgatgatgca
     7921 tctatgttaa ggcgtaagcc ttagcaaaaa aatccgataa aaatgcagtt aatcaaaaat
     7981 gaaagcagaa cgaaggatgc atgctatgca gacttgtaat catttttaat ttaaattcct
     8041 ggtttgctaa gatatactta agcaggtgtg ctaaaataga cctactaaat ccttgtcagt
     8101 gaggtggcca atgctcgtag agttattatc ccgcattcaa ttcggtttta gcattggttt
     8161 tcatattcta tttccaactt taaaccttgg ccttgcggtt tttcttgtta tcatggaagc
     8221 cctatggctg aaaacaaaaa atcccgttta tctcgaaata tgcaagttct ggactaagat
     8281 ttttgctttg acttttggca tgggggttgt ctccggcatt gtgttggctt atcaaattgg
     8341 taccaatttt ggacccttca tcactcagtt tggaaacgtc ctgggggctt tgtttgctta
     8401 tgaaacgttg accgcttttt tcctggaagc tggtttttta ggagtcatgc tttttggttg
     8461 gaaaagagtt cctccagccc ttcattttat cgccaccctt ttggttacca taggaacaac
     8521 cgtatccgct ttttggatca tgtcagccaa ttcatggatg caggccccca gcgggtatca
     8581 actcattgaa gggaaatatg ttgtcgatag ttggtggacc gttgttttta atcctacctt
     8641 tattcccaga ttcattcata tgctgttagc ttcttatgta acgacttgtt ttgtcatcat
     8701 ggcggtatcc ggattttact ttttaaggaa acaatctact gcaattgcta aaaaaagttt
     8761 atctttcagt ttatgggcat cgttgatttt agttcctttg caaataggag tgggtgatgt
     8821 cgtgggtatt aatgtgcatc attaccaacc actaaaaaca gctgctatgg aaggcatatg
     8881 ggagactcaa aaaggagcgc cgcttttgtt gtttgctatc ccgtcacaga cacagcaaaa
     8941 aaatttttat gaagtgggaa taccaaaact ggcaagcttg atcaatacgc atgagtggaa
     9001 cggtgaaatg atcggtttga gttctgttgg caatgaagag cagccgcgca tggcccctgt
     9061 gtttttttcc tttcgtatta tggtgggtat tgggctcatc atgttattta cggctatcgc
     9121 tggttttatc ttgcgcttaa gagggactct ttttgaaaat tcatggtttc atcggtggtg
     9181 tattgtaatg gctccactcg gttttattgc cagtatcgct ggatggttaa ccgctgaact
     9241 tgggcgacaa ccctgggttg tccatggtct tttaaagacg agagactctg tttctgcaat
     9301 aggggttgaa gaagttatta tttcttttgt cttgctcatt gttgcctatg gagtggtttt
     9361 tggattttat ctctattatc tgctgaggct catctctcat gggcctcgaa ttcttgacga
     9421 ggatgaagcg gaacatcatg cttttcaata tatgaccgat ttccccaagg agaaaaaata
     9481 atgttaccgt ttattttcgc tacacttctt gggtttatcg ttttgatgta tgtcatttta
     9541 gatggttttg atttgggaat tggtatttta tttcctttca cctcaagtga gagcgaacgc
     9601 gataaaatga tgaattcgat tgcacctgtt tgggatggca atgaaacctg gctggttttt
     9661 ggaggagcca tgctttatgg tgcatttcca caagtttacg gtttgctttt gcccatcctt
     9721 tacatgccta tcatgcttat gctgattgca ttgatatttc gtggtgtgag ttttgagttt
     9781 cgctttaagg ccaagaggtc aaaaactttg tggaactggt cattttcaat cagttcggtt
     9841 gcggccactt ttttacaagg agttattctt ggcgcctttg tacaggggtt ttctattaat
     9901 gaatcttcca tgactatcaa tgatccggac tggcttactc cttttagcct ttgtaccggt
     9961 atcgctttgg tgtgtgggta tgcgcttttg gggtctacct ggatgattat caaaagcacg
    10021 ggaagactgc agcgtaaaat ggttcattat gccagaggct tgttagtctt ggtcagcttt
    10081 tttctgcttt tcgttagtat ttggacgcct ttgcatagtg aggaagtgtt tcatcgttgg
    10141 tatagctttc caaatgtgct actactgagt cccttgccct tgatagcctt aatggctatt
    10201 tgcctcgtat ggcagaactt aagttccggc agtgaataca aaccgtttat ttacagtatc
    10261 attatatttt tatgctccta tataggaatt gcaatcagtg tctatcctta cctgattcct
    10321 catcaagtga ctctctggga ggcggcagca cctgattcca cacttgtttt tatcttgata
    10381 ggcgtcgcta tcatgctgcc aatccttctt gcctataccc tttatgctta ttaccttttt
    10441 cggggtaaat catcagaaaa ttatcactag ttattttctg ggagtcgaat tatttttaag
    10501 ccatttaggg aaaaacatgg gggtcacttt ttgatactcg atgtaaagat tgcctctgga
    10561 ttcaatcgaa ccttgttcag tcatgggagc tgtgattttt gtcatgatta aataaagggt
    10621 caatggagaa ataatggcta tccatccata aggagcggaa agggcaaata aggtaaagga
    10681 gcaccatacc agccattcat aaaaataatt tgggtgcctg caataacgcc aaagtttttg
    10741 attacagact tttccaggat agtttttctt aaaatcttgc aattggttgt cggcaactgt
    10801 ttccaaaatt aaggcagtta gaaatatcat caatgcgagc ccatccagta gatttaatgt
    10861 tgtttgcgtt tctgaggaag aaaaatacca gggaatggaa acaaggcaaa tcaatacgcc
    10921 ttgcaactga aaatttaaaa aaaatcccaa aggtttggca agtttccaat cgttgcttaa
    10981 agcaagatag cgcttgtcta tttttttaag gcgtattcgt gtccaccata aataccctcc
    11041 aagcctgatt ccccaggcaa gcaatgccaa gacgagcagt aaagagcgca atgaaaattc
    11101 ttgattattt aaataaataa gtccggcgag tgttaaacca gaagcccatc ccacatcaac
    11161 cacggaagga ttattggtgt aacggtacca tagccaaatc aagcacatat gtaaaaagat
    11221 gatggcgatc acaagtaaaa ttatcatctt attcatttcc tatgccggta attaaatagg
    11281 tataaactca agggcaagaa tagagaccag gttaacccta aagtaaggta aaatacaact
    11341 tcactgccga taagggttgc cgctccaaat tgtaccccta gccaataggc aaagataatg
    11401 gagggaaaca tgactaacgc ccaaaggaaa tagaaagtta accaggaacc atacaaaaca
    11461 acgagattaa atccgaaact tagccagaga gcgaccatcc aaagcggagg aaaatgagat
    11521 ccaaagaaat tggccttaaa atagatcata ccgctataaa gccagatggc atcagtgatt
    11581 gcacctaaca aggctagtcc aatggaaaaa tagagggcat cacgccaggg gagttgatta
    11641 agtatctgta caataatctg tagtcccatg attaatacgg taatgattaa tcccagcaaa
    11701 ggatagccat aagaggctaa gtagatacaa aatatccagc acagatagta cgacagataa
    11761 tgaactatat gcaaaggatt aataatcatt acactctctc aggcggcctg cgatagcaat
    11821 gcatccttga ccggcatgta aaccaatggt aagggataca gactctatgt acgtgggtgg
    11881 tttattgaat gcttgggttg tcatctcagc gagatgcaaa gcctggtttt tgtcacccgc
    11941 atgaataatg cagtaatcct ctaaaatgag ctgagcctcg tctttctttt cctgaaggaa
    12001 gttaattaat ttattacgtg ctcttgaggc tgaaaaacac ttgcctacca taataccttg
    12061 gcctgctgaa tcaatggaga ctatgggttt tatattaacc cactgggcga ttcgcccgga
    12121 tattttacta acccgaccag aacgaatcat cgatttgaat tggttcacca taacaaaaat
    12181 ataggtgttt tttatcgctt tgccgagtaa tttggttatg gtatcgatat cattccctga
    12241 ggcaattagt tgacccgcat aatgcaaaag caatccatgc gccccagaat tcgttttggt
    12301 atccagtacg gtgatattgg cgtatttttt ggttgcagta aggaaggcgt cataagtgcc
    12361 actcattttg ctggatatcg atagaacaag aacatgatta tagtgtgagg caatttttgc
    12421 cagtttttcc tctataagat ttggattcat acaagaggtt ttaggataag ttctgaacga
    12481 gttaagttgt gtataaaaat aattggcttc gaagcaatgt ttatccagta aatggtgctc
    12541 gtcgacatga atatttaaag gaatttgatg gatttgatat tcatcgatta aatcctgagg
    12601 caaatcggcg cttgaatcgg tgaccaaccc tatcttatat tttttcccaa actgcagttc
    12661 atgttgcctt tgcatgtcat ctactttagg gtattggata gtgtaatcct tatataaact
    12721 cgtgaaaatt tcacgaggat tattggtatg aacatgaaat ctggtgatgc gatcattgcc
    12781 cgtaacagag gcgcactctc cttgttcttt taaaaaatgt aataatttat ttttatcaat
    12841 ggaggcggat ttgagtactg cttctgtaca gtatcgatat tctgtcgtgg tgcatgtcaa
    12901 ctgggggtgc tctgtgatag ataacggtga gtgggtattt gctgtatctg tggtgtgtga
    12961 ttgctgtaaa aactgtgtaa acccttcaac gaaataataa aaccctaggg caccagcatc
    13021 cacaacattg gctttttgca aaatatttaa cgaattttta gtttgttcta cttccatggc
    13081 gagtaaaggt agcaattcca acatggcttg agctagcaag gaatcgggtt gttttatctc
    13141 acacactgta gcccactttt gaatgagtgt tatcatagtg ccctgtacgg gattagttac
    13201 gactgcggca atttctttac caacttgaac catgattttg gaaaagtcat taaaatagag
    13261 ctcttctttg aggggtaaat gcttggctaa taagttaaag aattgagaaa aaataatccc
    13321 ggaatttcca cgtgcaccga taatgctcgc gtcagcaata gactctagag tgcctcttag
    13381 gtcagctttt actgatgaaa aatagatgat tgccatgcat gtggaggata aattatcccc
    13441 cgtatctcca tcagctaccg gaaaaacatt aatggcgttg agtatttgtt tatggcgaat
    13501 aagcctatga cagccggcta ttattgcggc atgaaatgtt ataccagtta acttatccaa
    13561 tggcatggga tgtccgtata atgaatattg aagtttccaa gctaatccat catagtaggt
    13621 atttttatat taatgcaaga agaatgatat ggctcgctac ctggtaattg ctgcaagcag
    13681 tgctatcgga caatccgtga caaccctttt aaaaaatcgt ggtgatactg tttttaccac
    13741 agcacgagat aatcataaga taagccctga ttttcaactg gatgccactc attttgatgc
    13801 ggttttagat gtttttcgcc aagcaggtcc ccttgatggt gtagtgaatt gtgcgggttc
    13861 actacttctg aaaaatgctc acagcaccag ttttgatgaa tttcaagtcg cgataaatgc
    13921 gtcactcact acttcttttg ctaccattcg agctgccggt ttaactatga atcaaggagg
    13981 ttctgttgta ttgatatcat cagccgccgc tttggtaggt ttggccaatc atgaagccat
    14041 tgcggcggcc aaagcgggta tcattggttt ggctcaatct gctgcagcca cttatgcttc
    14101 aaacaattta agagtgaatg tcgtggctcc tggaatggtt aatagcccgc tgacctcatc
    14161 gttattaagc aatcagttag ttttgaatgc atcaaagaca atgcatgctt tagggcgaat
    14221 tggtgttccg gaagatattg cccacgcaat tgtgtttttg cttaatccgg acaacagctg
    14281 gatcacaggg caagtgattg ctgttgatgg cggattaagc cgagttcggc ccaagatgaa
    14341 aatttaatga aactataatt cttatgacga cactgtgttt tatattaggc gatcaactca
    14401 atgaaacaat cgcctcactt gcggcaatta ataaacagaa ggacattgta tttctatgtg
    14461 aagtggcaga agaaacttct tatgtccctc atcatccgaa aaaaatcgcg ttcttatttt
    14521 cggcaatgcg tcattttgcg caacaattga aaaagaaagg ttatcgagtc cgctatacgt
    14581 cattcgatga tcctcgcaat caaggcagtt ttgccaaaga attgcttcgt gcgattgaag
    14641 aggagcaggt gtcagcaata agccttactg aacctggaga gtggcgtgtt ctgaaaatat
    14701 tccatgattt aaaaagccaa ttgactattc ctgttactct acatgaagac aatcggtttt
    14761 tatgcaaaat taatgagttc agaaattggg ctaaggaaaa aaaacagcta cggatggagt
    14821 ttttttaccg gatgatgcgc caaaaatatc atcttctggt cgataatgat gggaaaccgg
    14881 ttggcggctc atggaattat gatgctcaaa atcgtaaaaa tgctaatcac atcagttcat
    14941 tccccaagcg tttagagcat accagggatg tgataactga tgaggttttg ggcttggttt
    15001 cagcgcgttt tgccaagcac tttggtcagt gccatccctt taatttggca gtgacccgtg
    15061 agcaagcatt agaagaagcc gattatttta tggaacattt tctttgcaat ttcggtgcgt
    15121 atcaggatgc catgctcaaa gatgaaatca gcttatatca ttcaaaactg tcgttttatc
    15181 taaatgccgg gcttttgttg cctttggaat tgtgtcaaat ggcagagcaa gcctatttct
    15241 caaagcaaat gcctcttaac tcggtagaag ggttcatacg ccaaatttta ggatggcgag
    15301 aatatgtgag gggtatttat tggcttttta tgccggatta cggcgagatg aactatttta
    15361 atgcatgcag gccgttgcct tccttttttt ggggagagga aacgggaatg ttttgtatca
    15421 aggaagtggt acgtcagacc tcgactgaag cctattccca ccatattcag cggttgatga
    15481 taacaggtaa ttttgccttg cttgcaggtt tagatcccaa acaagtttgt gagtggtatc
    15541 ttgctgttta tgctgatgcg tatgaatggg tggagttacc caatacattg ggtatggctc
    15601 tttatgcgga tggcgggctg atggccagta aaccgtatgc cgccagtggg aaatacattc
    15661 accgcatgag taatttttgc caatcctgct attataatcc caatgatctt ttgggtgaaa
    15721 aggcatgtcc ttttaatgcg ctttattgga attttcttca ccaaaatcaa gaaaaattaa
    15781 aagataaccc ccgccttcgt tatgcctaca tgaattggca aaaaatggat tgtgataaac
    15841 aaaagaagac tatccataaa gcacagcgaa tattggcaga cttggactct gccaatctct
    15901 gatgtcttat ttgatataac ttgccacata gattgtcgct atcgaactcc aggagcatag
    15961 tactataccc catagccaat caaggaatgc catgccgaca ggaaaatcct taaaaatagc
    16021 gaggcaggta aaatcataaa ttccataaat aatagcacca agaagcgcgc catataatgc
    16081 ggcataaggt agcgatgttt tagccagcgg tataacaaag acaacaatac tcagggcaaa
    16141 aagcagataa actaataaag cagaccacca tattggcttt aattgcccct ctgaaagacg
    16201 cagccactgt tggtattggt caaaataaag atttctggcg atgaacccca gccatatcat
    16261 atctgtcaca ataaagatta aaaaggcaat tgcaaatacc ttaagcgtcc atccattcat
    16321 tttattccct taatgatgat ttttatcctt ctggttttgt attttacatt caatcggatt
    16381 caggtaatag aacttcattt ccaaaatcta tcatttaaca aaaagttaat tttatgacta
    16441 atcgagataa atgcttctta ctatctataa aaggatatcg gtaattacca tatttgaata
    16501 tggtatttga tatttgtgca atcattctct taatgagccc tatcttggga gcagctattt
    16561 tgcatgcgag agcatgtttg ggagttatta ttttgctttg tatgttgctc ttaatttttt
    16621 cttaatgatt gttttttata atgcaaaggt tgtttttatt caagtttgta aatatgtgag
    16681 gttccttatg aaattgcttc ggtttcatga attaaaatca ttgccaggca tggacgagaa
    16741 agcattagaa ttgttgataa aggttttagg caacaaaggc attagaaaac taataaaatc
    16801 cgccgacggc aagcctatct cacgcgaaat aatgattcat gagtttggaa tagattgtca
    16861 aattctgttt attaccaccg aggcatctct taagccaatc atcgttccca ccgaaaacaa
    16921 gatttcagga ggtggtaaat cttattgtga gcaatttaaa gtttatgctt tggatgatgg
    16981 aaaaacttat ttcctaaaat ccgttaagat cgatgctgaa agcttgacag agtttactaa
    17041 tgagaaagat accttaagca aattgggtcg cctggtaggc acttttttta atgaacaaac
    17101 tcaggtgcat tatattctca caacttttat aaaagggatt gatttatccc ggtataaaaa
    17161 cgcacttcct cttaatgtta atctaaaaca tttttgggaa gttctcggca ttatgatttc
    17221 tgtatgccat caggtgaaac aattccatga attaggctta attcaccgcg atcttaaacc
    17281 tggaaatatt atgctggatg cggatatgca gtgtcattta gttgatttcg gcagcagttc
    17341 ttctgataaa gaacctaaac cggcatcatg gggaaccgca tgctatttag ccccagaact
    17401 taaagcaaat gaggatttca ttgcgttctc tcaggctagc gatctttttg ctttggctta
    17461 ttccctcaat gaacttttta atccattcag acaggttaaa tttgcgaaag tagatatagg
    17521 aattaaaaac aagcatcttg cgcttttgca cgctgaaatc gaggcatgta ttactgggct
    17581 catgtcaaat gaaacattag tacgaacact ttatttttct cgtatcctgc aattacaaag
    17641 agtcccggag agtttcaaat cgaaaccaga agcatatacc tacatgatta tgctgctcac
    17701 tcaatggaaa agctgttatg aagcaccgga aatgaacaaa gagttagatg aaatcattgc
    17761 agaaataaaa gtggcttatg aaaaccatga acaggatgcc gtaaagatta taacattgct
    17821 tgagcagtta tctaaagcgg acgggctgct taattctcat aaagccttgc tctctgtttt
    17881 gattaaatcg cttgctaatg tccagcaaca agaactcggg cacgacgaca tcttgccgcg
    17941 tcgctttgaa tcagatgttg tgagccacct gataaaaact ccaacagcta aaatgatggc
    18001 agcgattaaa caagtttcag atgctatcgt aaaaatcctg gaacaatatg aacattcccc
    18061 taccgaatct tttgagcatg aggttttaca agattttctc gaacaaatgg ttcaatcgga
    18121 cattcttttt ggtgccctca aagagaaaat tgagatttcc gatgttatag caatacttaa
    18181 ggcaaacagg ccggaagatt tcgtacaaat tcagcatatt tcttttaaat ttgcccaagt
    18241 tgctttacgc gatcttgcct tgcatgacct gccaaaacat gagccaaaag gcgcgttttt
    18301 agaagtattg aaaaaatatc aagcaaaata tgcgcctgaa gagacatccc tttttgagct
    18361 ctatcatcac atggcaggtg ttagagatca caacccatcg aaagtaaaga aaggggaggg
    18421 ataccgcttt agagcattca tttgtgatga gcttttttat ggagatgaaa tttttactac
    18481 cggggataat cgtggccgcg aaggcaagcc taaccaaaga ttacaaacta atcaagttgg
    18541 tttaatgaag ttcgaacatt cagcccacac caaaggacta ctgacattaa atggtcaatc
    18601 ctggtatgct gattgtaaga cccagctacc aaactacgat tcaattcatt atctctcagc
    18661 cctgaaaacg gactgccctt atattacggg gccttccggg atgacttcat tatttatgaa
    18721 tatgatgttt cttctgctca atcctaagga tcaagatgtc attctctctt acagccttgg
    18781 ggtaatgact tatgtagttg gtgctggcta ccacagcatt aaagaaattc ttattccgat
    18841 ggtcaagtgc gtaggtattg tgcctgatta tccacagcat aaaggaacag agtgtcttac
    18901 agccccgcct ttatataacc attactttaa ggcaatagag gagtttgata aagagtttgc
    18961 tgaggctcat gaaaaaattt ggcaggaata ccttgcatat ttccgtatga cttatatgcc
    19021 cgtttgtatg cgtagtcatt gcctgccaca tcaagtcccc tcaatagagg atataagctc
    19081 agaagtaaaa gaaatgcttg aaattgtgtc taagtctgtg aaaaattgct tagaagaaca
    19141 taaagttaag cgtactggcg atatggggtt gtttctttca acaccatgtg ggcaaacaaa
    19201 ggcgcttgag gtcttaagtc atgtctgtca acaacaggta gggctaaccg caattttcag
    19261 gcagatccag gcgtatttta aaggaacttt tgagacagaa gaaggaataa taataaatag
    19321 agaaatgcaa cttaaattta ttgctcattt ctttgatgta ttgaaggaaa gcccaacact
    19381 gctttcacaa ttcaatttga ccttaaggat agaaaatacg attggtgtgc atcagtgcga
    19441 attgggaagc tctaagcaaa aagagctctt cctggagatt aagaagacgt ccatcgcagc
    19501 aaatgaagag cagatggttg caaatcatga acccaggaaa gaaaaccgca acgtcatcat
    19561 tttgccatat taacctcaag gaaggcgttt tatgttaagt aaagaaaaaa ttaacccttc
    19621 tctaaaaaat acaaaacgcc aaggcagcga taatcttgct gccatggctg atccggcatc
    19681 attaaaaaac acgggtgact accgatgttt taattcaaaa ccagaggctt acacttattt
    19741 tgttttttta ttaaatcaat ggcagaccaa ttacatggat gacaaaagag aagcgatagc
    19801 taaagccatt gattcaataa aaatggctta tgaaaatcat gagcaagata ctttggtcgt
    19861 tcacaaaatg ttacatggct tgagtcattt gcttgagcag gagcatgccg atgagttaaa
    19921 tgcgttatgc ttggtgccta tcaaagacag tcaatctaat gcaaacaata ttctgccccg
    19981 gagatttgaa tcagagctgg cttgccgcct gatagaatcg ccaacgaaaa gcatgatgca
    20041 agcaattgat ctggtatctc aagctataat caatttaatc gaagagtata gtaaattaga
    20101 ttcaaaatct cccgagcgag ctacgctgaa tttttttctg caagagttag aggggtctga
    20161 tatagagttt ggggctttta aaaaaaggcc gtctctggaa gaggttattg ctattttaaa
    20221 ggaaaataat ccgaaggatt ttattcaaat tcaattcata tcctttatgt ttgctcgcag
    20281 tgtaatcgga ccatttccat tacgaaattt gccaccattt aagccaactg gcctttttgc
    20341 ccaagttctg gataagtatt tacaggttca ccccgatttg aaagaagaag aggaaggctt
    20401 ttcctatcag cttatggcag ctaaaaatga ggcagaagaa tttagaactt tcatcactaa
    20461 aaaattattt taccaggatg aactttatac tcaaggagaa aacagagggc gtcaaggacc
    20521 tcgtaaaacc tacttaaaaa ccaaccagtt aggattgatg aagctggatc attcagcgca
    20581 tagcgtgggg ttaaccgtta ttcctgaaca aatttggtgt gcggatgctc aagcacaaga
    20641 agccaatcag aattcaatat attacaagac tgcacttaca aatgattgtc cttatgtcac
    20701 tggcccttct ggcatgactt ctatctatat gaacatgatg ctgctgcttt taaatcctca
    20761 aaatatcgaa ttaatacaag cctacagcct tggaattata gcgtatatcg tcggtgccgg
    20821 ttttcacagt gtcaacgaaa ttcttattcc cctggtgaaa tgcattaacc ttctaccagg
    20881 ttatccaaac tacgaggggc ttgagtattt aacccatcct ccgctttatc atgaatactt
    20941 taagcaaatt gcacaatttg attctgattt tattcagtta agagatcaag tatgggcata
    21001 ctatctaaat tattttaaat cgtgctatat gcccttatgt atgagagatt tctgcgaggc
    21061 agaacagctt gcaaattgtc ctgactccgc ccctgaagta aaaatattat gcggtattgt
    21121 gcttaaatca atcgaggact gcattaattt tcataatctt aataggcatc tggatagcag
    21181 gtttttaggt acgactactg cggacattga tgctcttaat caattgaagg aggcttgctc
    21241 gagtcaatct tcaattagcg gtattatgcg attattgcaa aaatattttt caggagaaaa
    21301 tattgctcgg ccaacagcga tgcataaatt tgaaactcgt tcttatatcc gttttttcct
    21361 ggatgcttta aaagataacc cgcaaatcat atctcatatc aatgagaaaa tgaaattgga
    21421 ccctccatta gtaatcagta caaaggctga aattttttca gatgattttc gaatagagtg
    21481 ctttaatgtg catcgaattc taaaaagaga acaccaggtg agtgaaaatt gtaataacac
    21541 tagccagttt ttaaagttgt aaaggaaaaa gggaaatagt ttaaattttc c
//
LOCUS       DBNCWK010000042        21246 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.042, whole genome shotgun sequence.
ACCESSION   DBNCWK010000042 DBNCWK010000000
VERSION     DBNCWK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21246
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.042"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..358)
                     /locus_tag="AC06IO_001394"
     CDS             complement(<1..358)
                     /locus_tag="AC06IO_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HIO9874593.1"
                     /translation="MKRLIICNGNKLTVCTQAISSGDIVEKYTPIFSLTKESDNELTL
                     ELSGIARGYYIIPSELSSSQEKAAHLITLLTRAEESQVTDMHKILNSFVSGKITSGSM
                     FNFENDGSFKREPEEAY"
     gene            1217..1459
                     /locus_tag="AC06IO_001395"
     CDS             1217..1459
                     /locus_tag="AC06IO_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874594.1"
                     /translation="MTNKILTERLNNELDELGVPCLMTERVQVCSKLFNLPKFKMEAL
                     LNGVIAIDANSIQKIADELEVNKDWLLGGIKKKTKH"
     gene            1930..2823
                     /locus_tag="AC06IO_001396"
     CDS             1930..2823
                     /locus_tag="AC06IO_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HIO9874595.1"
                     /translation="MFSLKKTTAAILALGSSALFAGTMGPVCTPGNVTVPCERTAWDV
                     GITALYLQPVYDADWGYNGFTDVGGWRNWHDVDLEWDWGFKLEGSYHFNTGNDINVNW
                     YHLDADTDHWAFVDQLHAYDITWDAVNAELGQFVDFSANKKMRFHGGVQYAYIKTDVN
                     RHLNGFFLDNFNSKFNGFGPRTGLDMNYVFGNGFGIYAKSAVAILVGTSKFLDNCTVC
                     GYSYGSKNAIVPEVEMKLGADYTYAMAQGDLTLDVGYMWFNYFNALHNTAAVNVGLGA
                     SLETDFSASGPYIGLKYVGNV"
     gene            3053..4771
                     /locus_tag="AC06IO_001397"
     CDS             3053..4771
                     /locus_tag="AC06IO_001397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874596.1"
                     /translation="MPSCKNNPLSDKTLTADLRNHLICKHLNLETIGKMSIASKHDLG
                     FFGVKPSWATLIQDYFGITHEELCFLSKNDANFHYQELFKHRLLLKENELKLRAGVQQ
                     CYLAREFRLQLLACCSNNAEFALNVLPQEEDDLWLDVAVCAQADRVYQSLFERNKERY
                     QENIPDIFLQTGLAPLRFICKTGFRPNKEQALTIIRSGNMNSVLFILEPENDFDLRPD
                     NNWLVVAVVSGNTLILEHLVSAYQLKPSLQLLMTAFVLGSQPMIDSITRLLNLPASTD
                     IIEVGHILGKNIISDYLTQIARTGNLTVLRIFIEKLGIKPNELLLVDAAYSGNLAMIE
                     YLIKQCTLELNERVFEQMLRAAAGAGKFELVIHIINHFKVRPDPMVLDKAAQSGNLIL
                     VRYLIDAFDLIRQHNLNPDDLIYFDEESDHTNSLLYHYLLFQFVFFRNPLKTLPSCPG
                     NSELHFLNVMHKFSPRFLIHFLVTTLNETDKNQMGMSNLDELFNQLSQLIHTHKQKYS
                     LVERNSMIETLKKLNNRWMSRDIMKIHSKILNCLKTEKYNWTADSDEIQENRKSHHSW
                     SYCSIL"
     gene            4944..5575
                     /locus_tag="AC06IO_001398"
                     /pseudo
     CDS             4944..5575
                     /locus_tag="AC06IO_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1975 family Dot/Icm T4SS effector"
     gene            5572..6435
                     /locus_tag="AC06IO_001399"
     CDS             5572..6435
                     /locus_tag="AC06IO_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIO9874597.1"
                     /translation="MSLASYKINDRTPLGTTIGERNHPLPIIPLPYTMKTVKQIHSGI
                     WHTVIRGYDQYNRPQIATFGKNYDGQLGTGDWENRDTPTLIKLPPEMFTLHVIETSFF
                     HTIAIGLDKNGFPIVASCGYNSDGQLGCGDKDNKNTLIPIKLPENMKSVEMVATGVKH
                     TVIVGKDINNKPVVATCGHNGYGQLGTGDEQNRVCLTPIPIPKDLITIDYVEAGAYHT
                     VICGRNIKNQPIIALCGCNSNGELGFAPQKIAKKSSLMTNPALFFPPRPDKGTINNLM
                     KISKFSPCNLL"
     gene            6628..7212
                     /locus_tag="AC06IO_001400"
     CDS             6628..7212
                     /locus_tag="AC06IO_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIO9874598.1"
                     /translation="MPTPKKILMIVGDFNEDLEVYFPYQAMLMNNYLIDTVSPGKNKG
                     DFIQTAVHDLDKPHLQSYTEKLGHLFPITADFSKITLKDYFALIIPGGRAAEYQRLNK
                     DILAIIRHFHDTNKPIACICHGIQILAEAGILEDKKCTTVGFCEPDVRKAGGHFIDTG
                     MDGVVVDGKLVTGATWLGNAPWMRAFLHILQNEG"
     gene            complement(7283..9217)
                     /gene="setA"
                     /locus_tag="AC06IO_001401"
     CDS             complement(7283..9217)
                     /gene="setA"
                     /locus_tag="AC06IO_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SetA"
                     /protein_id="HIO9874599.1"
                     /translation="MYKMYSYPGWRIDMKTENLPQAGQEAQIDKKIHFIWVGHIMPQK
                     NIQVVSEWAEKNPGYETIVWVDKKIAPAKELGLFIQDMKSKGITVKDINEEGVCRDSI
                     RHELDQESPNYGMASDMLRLNILAAEGGVYLDSDILCSTPFPDEIHAPFGFLLSPWSQ
                     GTNNTLCNDIILCRKGNQIIRQLADAIEQSYIRRDSFEFTHEYASMKETRGERIAKTL
                     GVTGPGFLFHQLKKMEILNDKSEIEAIHWELQDQRYLIDGSVEAPDYFYVPQNNKNDA
                     SWVPSIKRPGIENMSFQERLENAVQLIAFDIQKTGLFNLDHYANELKVKQNSWCIAAE
                     TPSELKPDSYLLIRPRDKTGEWALYYVDEEKKLNPVTLPVIKGAIKLSEFSDPLRKFH
                     KVLSQVSDPVNPTAQELKQIGRALMDLEPRQNEWRCKNKWPGVEEIAQELWQRVTSND
                     TLRAQIKQCSTQLESLKPRVAELSLTRASGAETEVEAHESNVKEQEVILQNTVVEEGT
                     QEKNSAQLVSENSSDEKIKTAHDLIDEIIQDVIQLDGKLGLLGGNTRQLENGKVINIP
                     NGAAMIFDDYKKYKQGELTAESALESMIKIAKQSNKLNRHTFFNQRQPETGQFYKKVA
                     AIDLQTTIAAGYDNNHGLRI"
     gene            9453..10820
                     /locus_tag="AC06IO_001402"
     CDS             9453..10820
                     /locus_tag="AC06IO_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5624 domain-containing protein"
                     /protein_id="HIO9874600.1"
                     /translation="MKTLMTCIFSLIILFSQPLKPIVFAHTNNTNNQGLSASANNSVG
                     SNLPNNIYVTPQAFWNLYFDFTGDETPGYPKGKINISQTLFQSEMKKNRSLAQQNEGQ
                     LILFINSTLYIYNSERQLELKQLMRTAPNSGFTEMTAISHIGPALMYLAKIKENGDAS
                     WKSQMENLLKDIQAVKVINAQTPSNWLEQVNAPAWKPHLTTIHNMIDYACSMAGNYMS
                     DVLNEKLSFDMASLQNDFLNGNKTYPIAYNNVMIGTFMLTALQSMDQLHSKISQLKID
                     WPHAKVIIRFVAGSNVSAGVTKGSNWLVPFVQALSNNTLATDRIYITPYAAVKPSLGA
                     QELTQADYNYYNNTVWDARHNRRIIANEVFTNITSIFLPDRPAIPGDYTYSKPPKIED
                     FLMRLKFSLAEPTEMLSNTVGFWMAGELAEKNWNYNKISIPGITTGFPQGISTYPNNN
                     PVIQR"
     gene            10907..12571
                     /locus_tag="AC06IO_001403"
     CDS             10907..12571
                     /locus_tag="AC06IO_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874601.1"
                     /translation="MKDFVIVGAGPIGLYWAARLAQIKESSGLPINIVVIDPRAGNYN
                     RERIVSNEAIKSISSRFSISMPSGKGIPSEAMYIRDFEKSLYEYCESKGVTFKKGTFS
                     GLRDNLVSYKDNKQNIRTVKCDMVVDCSGSNRMVLKETNRLLGKEVFEIKTLGKNPHT
                     NHFSCYMSLSTEEAKKLLIPDEIDPLLQAKQLCKLRQKYDWPNFSFPVVDIRSAKNEE
                     GGVNYFIYYEVPDNLKEKDKSVQIEFLKDLLKLKYGFEDIDFTIHSFGHFPVCPKYVD
                     KPFYLGEDLPVVLAGGDCQIEPDYRKGIGIESGIERANFLFDTVHGTSKGLGFLFDNY
                     YQQVARYVGYHGNLIEQFYLQRVDNIKGSSLEQAKKILCSACGSVKEIEDVAAIASEL
                     KLLGNELFKKPNYESALECYLNAIHLYQSFEKALPLTMDFVTLHSNACQTCLKLKKYE
                     QCINLANEGIKAYAEIKAEDKEMLFKLLFRKASALVELGNAFDVKTQRKEFDEALKDL
                     KETYELMQEHSGVNNTAFVKQIQTKIVTIEKKLPPPQEEINKIEFI"
     gene            12737..13936
                     /locus_tag="AC06IO_001404"
     CDS             12737..13936
                     /locus_tag="AC06IO_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HIO9874602.1"
                     /translation="MLNKGYIKTTPYGLGFSELFFEDNLKKYFSDPSLEVLSAEYSPS
                     ASDKMAWSAKHPKQGDHPIGVQRYKISFKKNGKIQTRHVIVKSKISDTHYLQVISNAF
                     TKCGIQTEMPILDYLSQLEFSNLNRKEITIYNMQHSFEAFQYAMPLCYGTYLDEKEEA
                     CVLILECLDEEWLSLDPFNTSKWDQTAIEALIDAIAPMHAVWYKKEKTIAEIKGFKNP
                     LNTDQMQRFIPYWNALEKAVKTSNLPFLTKEDYLFHTHLIETMPQWHIKIDAMAKTLV
                     QNDCVPKNIALCNQNGAKKVLVYDWEIATIHIPQRDIVEFLSYVLPENFDSTLLGNYI
                     ERHRVKLGETIKQPIDATEWRLGFQYALYDYLIQRVFPQIPFEKLEARNIAKVYNNAR
                     RMMALLN"
     gene            13956..14693
                     /locus_tag="AC06IO_001405"
     CDS             13956..14693
                     /locus_tag="AC06IO_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIO9874603.1"
                     /translation="MTHIRVILPGYNTKVLNKEMLKPFESPGLELSVDYADIAVDAVR
                     HEFDIALMAPYLIKKVIKAEQEKVHAIVIDMMADVAMRALREAVTIPVVSLAETATHV
                     AAMMGNNFAIINTTDELTPIQESLVRSYGLYDRLACIQGIQLDPHDDYRSEKIIHGLV
                     EQCADAVIKKRADTLIFGSGRLIGYREVIQKGLEEKGLHITIIEPLPTGIYFAKFLVD
                     SKISQSKYVYRSPDASTLKTYSKILGD"
     gene            complement(14733..16046)
                     /locus_tag="AC06IO_001406"
     CDS             complement(14733..16046)
                     /locus_tag="AC06IO_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9874604.1"
                     /translation="MNSIPHLILGDIITPTQAGQSLLIKKGYVLIDGDSIIEVGEQAH
                     LNQEISYTVSNYPDHLILPGLIDTHSHLSQYAICGAGDLPLMDWLNTLVFPAEAAFSS
                     NLQRCQRYAELYMHACLGFGTTTINTMVTSSRQATEIVCDVAARIGIRAFIGLVLMDR
                     NAPDHLLVDCDQAFKDLTVLKDRYHGKNNLHITVSPRFAVTCSATMLKQAGEFARANK
                     LLLHTHLDKDEGFDELIQSLFSTAHDYFDVFESTQCIADKTVFAHGTLLSLHEMKRMG
                     DYAGQVGISHCPSSNFSFAMGMAPVSFFKGLGIEVGLGSDSGGGDSLSLFDEMRSASF
                     TNKALWRLDKKTALIDAKSWLYHATLGGAKLLGLEKQIGSIEAGKKADLIILDDRNNY
                     PLAELSSLSQELALDELQYRLARVIARSCENKLVAVYIDGKKVYQ"
     gene            complement(16216..19077)
                     /locus_tag="AC06IO_001407"
     CDS             complement(16216..19077)
                     /locus_tag="AC06IO_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1986 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874605.1"
                     /translation="MSKFSLHLSEVISEVIGMGKTTTPEINQLNKYVIDKKSIKLAND
                     LWARSVPLKGTPAERYIVGTRKIPAGVAEQLQIRSLKGPIGIPEFDKNKPYKDYVVAP
                     VYNLDDELVGLQIIQIDPDGNKATNPESSHFFCKRYLGQANPLREGKAAVINKTDNVD
                     VVFIAEGVETAASVASLDEVRNNYSVLASMGVSQLPSTLAYVKTHYPPGATVVLLKDH
                     DDPVANPLANLEFEKACKALVFAGYKVIVKEPPNLNDDWNDVIVRGGVELLTRQFNLD
                     NPVLIASDNPARNSKVTPHFKTLYADLLKNEKLAEEQVVCGLLDKVIGQLIDKLKKTQ
                     SSDDLHKTIKEIDEVARTTEGVRTLLPETKNIPYQLRGLRRALKELKFLEDRRRDSLR
                     EDEDIDFSTDPQTNSNGDLFKAYSYVMSHCLKHLQTLNPSDVFDLKKHSEILGFYERR
                     LNNIDNEIKRIPQLISEFGSDDEIVIKQLKNKLKSLQLERKLYAQEFHEMKQQLHLLR
                     SAHPKGNTPYANYYKQFVEEANSIMMKGKTQTKAIREELTGVWANRKQELQEDYKKRI
                     EAARQHFVSERKKAIPPMIEYLEALRVIVKERSKEIDGKSQEKENTASASESRNYQER
                     YLRAMAELGQFEGMDKILQDWLNNLENFKTVSPLIYYPPQEGATVEGVELIDYDSDGE
                     ETLDDLRKKIFDDEESLLESPINDREKGKEKLEGKAGDSDPQVMSGPSHLAEGLMQSI
                     QSSSLKTSDPQLYLTIKDFSERLAVGLYKSFEVISPESGERQEFDGLALRKKGLTIIE
                     RKTNDGTGPGLFQRNFCQQKIVSKHDFVLKNMIEELKAQSHPEKFIDIDVPKPLDWYS
                     ENFTPEMQEHLANAAKSMVVKALQNLSFEFNVNRPKAYSETDYRGLFFNRSLIPGGVS
                     LRFSRVGKGNEEVAHQHMDKISKERTAERESRILPMRGQ"
     gene            complement(19160..19321)
                     /locus_tag="AC06IO_001408"
     CDS             complement(19160..19321)
                     /locus_tag="AC06IO_001408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874606.1"
                     /translation="MKIYLVQHGDSLDKDIDPERPLSDQSQKDIQLLSIYALFIMCII
                     GYSNLLSDK"
     gene            19396..19941
                     /locus_tag="AC06IO_001409"
     CDS             19396..19941
                     /locus_tag="AC06IO_001409"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIO9874607.1"
                     /translation="MKPKISVFIATSLDGYIARNDGSIDWLIEANSLASPGEDCGYRS
                     FIATIDTIIMGRYSYEKVITFDEWPYGSIPVIVLSSKPIQIPEHLRHIITVSNQSPPD
                     LVSELSLQGIQHVYLDGGITIQGFLQNNLVNEMTITVIPVLIGSGRSLFGSLKNDIKL
                     THQLTTSYPGGFVQIKYQISA"
     gene            20040..20456
                     /locus_tag="AC06IO_001410"
     CDS             20040..20456
                     /locus_tag="AC06IO_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIO9874608.1"
                     /translation="MDIRYLIYAWGSSILFFIYLYLSYLVIIQRRKFHVPYLTNDDKA
                     FIARFRAHANFAEYVPFALILMGISIMFQIPPLLLAILMFALIIARLGHAYGLIKKEA
                     KKQFKFRIYSVATTFIIMGILAIFNVSKATILYIGI"
     gene            complement(20615..>21246)
                     /locus_tag="AC06IO_001411"
     CDS             complement(20615..>21246)
                     /locus_tag="AC06IO_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874609.1"
                     /translation="VLVAYTKFFAINPYDKKSMSIYQSVKKEFEKDLNHIESSYSELL
                     KDKDFNSALQVEIKELELRLKYVLFNWFFLFKKDKQDLSKQICQLKVDVLRLKTKFMI
                     SHILLNHDKYNLEHYKFVVKHLHNHLEDFNDKIGLIKDIEVDIYNDLFSEMYEWFEQV
                     YSLLNNRYDEFKEEFSIGDKWDNWSYGNSSSEDDWDDNEPLVFLNNTIP"
BASE COUNT         6745 a         4175 c         3709 g         6617 t
ORIGIN      
        1 tataagcttc ttcaggttct ctcttaaatg aaccatcgtt ttcaaaatta aacatgctgc
       61 ctgacgtgat ttttcccgaa acaaatgagt taagtatttt atgcatatcc gtaacttgag
      121 actcttctgc gcgagtaaga agtgtaatga gatgtgctgc tttttcctga gaagaactta
      181 actccgaagg aatgatataa tatcctctgg caattccaga caactctaat gtgagctcat
      241 tgtcagattc ttttgtaaga ctgaaaatgg gtgtatattt ctcaactata tcaccagaag
      301 aaatcgcttg tgtacatact gttaatttat tgccattaca gataatcagt cttttcatta
      361 gttagctcac attttggaaa aacaaagggc tttgattata taaaaataaa accaaacagt
      421 aaatatttga tcttttatga ttacattaaa actattagat cttataaaaa tcaataaggc
      481 caaaagatac cataataaat tgaatcaaca ttgatagaat agaccttttg cataaactaa
      541 ttattttatt ttaggacaaa gcgcatatat tttaaggagt ggagtttata tgcagtaaaa
      601 caaaaccaac atgagcacgt tgcttacgtc ctggccttca aaaaatttct tagtaaacca
      661 agggctgtct atgaatatac aaagagggaa cccagtaaag ccaagctaat ttgcagtgga
      721 actatgatat ttttattcaa agttaatcaa tcctgtcctt ttaaatcata caatttcaac
      781 acatctcatc agtaaataat gtcacttaaa ataatgtcac atagaaaaat aatctaaaaa
      841 tactgaaaat actgacaatc cagattggag ctttgctttc tgaattcaaa gaaataaaaa
      901 tgccctaaaa taatttcagt tatttctcaa aaatggcgct gcagatatac ttcatgaaga
      961 cagcattcgc tgattctcag cttgtttgaa aatagataac aatcagacgc agtgtttgac
     1021 ctaaaagata atagaatctg ttttatctac tcaagcgaac atccctttca aatcaccaaa
     1081 gcattcaccc ttggattttt tctattttca gagacaggga tcaggctttc aattaattat
     1141 ctgacaaaaa ttaatttgtt aatcaatcct taagctttag tgttttacac tgatataaaa
     1201 cgagaaggag aataccatga ctaataaaat attaactgaa cgcttgaata atgagctgga
     1261 tgaacttggt gttccatgtc taatgaccga gcgtgtgcaa gtgtgttcca aactatttaa
     1321 tttgcctaaa ttcaaaatgg aagctttatt gaatggtgtt attgctattg atgccaactc
     1381 gatacaaaaa attgccgatg aacttgaggt gaacaaggat tggctgctgg gtggcattaa
     1441 gaaaaaaaca aaacactaga ggctgttgac attttctcct acagctgacc tactgcggct
     1501 ttgtccgcag tatcctatag acttgactca ggatctctgt atttataaag gcactaatcc
     1561 atatttatgt tgccaatttt agaatagcat tttcctgaaa tggcaggagc taatgatttt
     1621 ttcgaatgat atcttaaatc tccccactat cctggctttt gtctaccatt gtaaattccc
     1681 tggcatcatg tgcttctaca cccgttactt caaaatccaa gggaaaagca ggctaattgt
     1741 tgaagtataa atcaacaacg gcgcatactg atcaaatctt atatttaatg cttaaatcaa
     1801 tgagttagat aatcttaatt taataaaatt acctctatta tttgatgaag aatcaaattg
     1861 attctgttag aattggttta tcctagatgt tcaaaggaat aacaataata aatcagtgga
     1921 gaacaggata tgtttagttt gaaaaaaaca actgcggcta tacttgctct aggaagcagt
     1981 gctttgtttg ccggtacgat gggaccagta tgtactccag gcaacgtaac tgttccttgc
     2041 gagagaacag catgggatgt tggtattacc gcgctctatc tgcaaccagt ttatgatgct
     2101 gattggggct ataatggttt tactgatgtt ggtggctgga gaaattggca tgatgttgat
     2161 ttagagtggg attggggctt taaactagaa ggttcttatc acttcaacac aggcaatgac
     2221 atcaatgtca attggtacca tttggatgcc gacaccgatc attgggcgtt tgttgatcaa
     2281 ttgcacgcct acgatattac ttgggatgct gtgaatgctg aactgggcca atttgtcgat
     2341 tttagcgcga acaaaaaaat gcgcttccat ggtggcgttc agtatgccta catcaaaaca
     2401 gacgttaatc gtcatttaaa cggcttcttt ttagataact tcaattccaa attcaatgga
     2461 tttggtcccc gtaccggcct tgatatgaat tatgtatttg gtaacgggtt tggtatttat
     2521 gcgaaatcag ctgttgctat cttggttggt acaagtaagt tcctggataa ctgcactgtc
     2581 tgtggatatt cttatggttc caaaaatgca attgtgccag aagttgaaat gaagctggga
     2641 gcagattata cctatgcaat ggcccaaggc gatttgactc tggacgttgg ttacatgtgg
     2701 tttaattact tcaacgcatt acataatact gctgccgtga atgttggatt aggggcttca
     2761 ttggaaaccg acttctctgc atctggcccc tacataggct taaaatacgt tggcaatgtc
     2821 taaataaccc aaaattatgc tgctaatcta gtcatgatca agcagcagta tgcaaaaaac
     2881 ccgtattgac ataggctaat acgggttttt ttatttcttt ctgtttaatt aaattagtta
     2941 tcctctccaa acttacttgt aatagtagcc atccaaaata aaatttggta aaagcatttc
     3001 aatttatatc aagcaaacac tattgcaaaa aaatcgtgag aaggatcatg tgatgcccag
     3061 ttgcaaaaat aaccccctgt ctgacaagac tcttacagct gacctgcgaa atcatttgat
     3121 atgcaagcat ctcaacctcg aaaccattgg aaaaatgtca atcgcatcaa aacatgatct
     3181 tggatttttt ggtgttaaac catcctgggc aaccctaatt caggattact ttggaatcac
     3241 tcacgaagaa ctctgtttct taagtaaaaa tgatgcaaac ttccattacc aggaactgtt
     3301 taagcatcga ttactgctta aagaaaatga gcttaaattg agagcaggag tacagcaatg
     3361 ttatttggct agagaattcc gactgcaatt attggcatgc tgttctaaca atgccgaatt
     3421 tgccttaaat gttttgccac aagaggaaga tgatttatgg ttagatgtcg ctgtatgcgc
     3481 tcaagccgac agagtttatc aatcactatt cgagagaaat aaagagcgtt accaggagaa
     3541 cattccggat attttccttc aaactggact agctcccctg cgttttattt gcaaaacagg
     3601 atttcgccct aataaagaac aagcgctaac catcattcga agtggtaata tgaatagtgt
     3661 tctgtttatt ctggaaccag aaaacgattt tgacctgcgt cctgataaca attggttagt
     3721 tgttgcagta gtttccggaa atacattgat tcttgagcac ttagtaagcg cataccaatt
     3781 aaagccttca ctacagctgc tgatgaccgc cttcgtttta ggcagtcaac ccatgataga
     3841 ttcaatcact cgacttttaa atttacctgc ttcaacagat atcattgaag ttggacatat
     3901 tcttggtaaa aacataataa gtgattatct gacccaaatt gctcgtactg gcaatctcac
     3961 tgtactgcga atttttattg agaaacttgg aataaagcct aatgagctct tattagttga
     4021 tgcagcttat tccggaaatt tagcgatgat tgagtatctg ataaagcagt gtacccttga
     4081 attgaacgaa cgtgtttttg aacaaatgtt aagagcagca gctggagctg gaaaatttga
     4141 attagttata catattatca atcattttaa agtgcggcca gacccaatgg tattagacaa
     4201 ggcggcccaa tcaggaaatc tgatactagt tcgttattta atcgatgcat ttgatctaat
     4261 tagacaacat aacttaaatc ctgatgattt aatctacttt gatgaggaat cagatcatac
     4321 aaatagtttg ttatatcact acctattgtt tcaatttgtt tttttcagaa acccgcttaa
     4381 aacgttacca tcctgtcctg gcaatagtga attacacttt ttgaatgtta tgcacaaatt
     4441 ttcaccgaga tttcttatcc attttttggt taccacatta aatgaaacag ataaaaacca
     4501 aatgggaatg agtaatcttg atgaactatt taaccagcta agccaactga ttcatactca
     4561 caagcaaaaa tattcactgg ttgaacgaaa ctccatgatt gagactttaa aaaagttaaa
     4621 caaccgttgg atgtcgagag acattatgaa aattcatagt aaaatcctga attgtctaaa
     4681 aacagaaaaa tacaattgga cagcagattc tgatgaaatc caagagaata gaaaaagtca
     4741 ccactcctgg tcctactgct ctattttatg atagcctgta attggattga gataatttga
     4801 tgcacaaaca atgattagtc gccctgttgc gcaatacccc taatgtaaca aaattatatc
     4861 cattatctga tagaaaaact gcagaaaacg taataagctc cttatgctgc ttgaagaaga
     4921 attcattttc cattctgaag atactggatt ataataaaac aatataccac ccaccgttta
     4981 taaaagtaaa acatgcctga tgttcgcggc agccctgatg caaaccaaat tccagatgat
     5041 atctggcttg acattcttat caattatctt caaccacaag atattgtgag cctgtctgaa
     5101 gtcaacaatc gcttgagcag acttttcaaa actcaacaca ctcaatctga gcaattgaac
     5161 cgaaaaaaaa cattgggaat tctgaaagta tttgcaggat acagttccac tttcctgttt
     5221 gcacgcctca atggtaagca aagattattt aaaaccagaa attcgagtgt ggtggatagc
     5281 gataacataa acccaatatt aatgcccatc tctctgccag agaacatctc ttttattgaa
     5341 tccattcaat caaaatccaa ccacaccgtg cttgttggtc gagacagtca tgatcatatt
     5401 atagtggcaa caaacaaaat caatcaggat tatgcagggc aagaaaccag tcacaaccaa
     5461 gtaatttccc ttccattaca cgacaataac atttctctgg tatctgccgg gaatgaagag
     5521 aactatattt taaaccctat ttttctcttt tcccgcaata caagagggca attgagtctt
     5581 gcatcctaca aaataaatga cagaacacca ttaggaacca caattgggga aagaaaccac
     5641 ccgttaccca tcataccatt gccttacact atgaaaacag taaaacaaat acattcagga
     5701 atatggcata ctgtgattag aggctatgat caatataatc gaccgcaaat tgctactttt
     5761 ggaaaaaatt acgatgggca attaggcaca ggcgattggg aaaacagaga tacacctacc
     5821 ctgataaagc ttcctcctga aatgtttaca cttcatgtaa ttgaaaccag tttttttcac
     5881 accattgcca ttggacttga taaaaatgga ttccctatcg ttgcaagctg cggttataac
     5941 tccgatggcc aattgggatg cggggataaa gataataaaa acacattgat tcccatcaaa
     6001 cttccagaaa atatgaaatc agtagaaatg gtcgccactg gagtaaaaca caccgttatt
     6061 gtgggaaaag acataaataa caagcctgtg gttgcaacct gtgggcataa cggctatgga
     6121 caactgggta caggcgatga acaaaaccga gtctgcttga ctcccattcc aattcccaag
     6181 gacctaatta caattgatta tgttgaagcc ggcgcctatc atacagtcat ttgtgggcgg
     6241 aatataaaaa atcagccaat tatagcctta tgtgggtgta attcaaacgg tgaattgggg
     6301 tttgcaccgc aaaaaatcgc aaagaaatca tccctgatga ccaacccggc cctgtttttt
     6361 ccgccacgac cagacaaggg cacaataaat aatttaatga aaattagtaa attctcgcca
     6421 tgtaatttgc tatgaggttt aatttatttc tacagagaaa aatgaatgtt caaaagcccg
     6481 ctatttctgc tttgtttttt gttatttctc tgtgagaata aaaccaatct tccacttact
     6541 tacaattatt tacacgattg tttctggaca aatttaatta atcgccacca tacttaatgc
     6601 attcaatttg taaacaagga atgtattatg ccgactccaa aaaaaattct tatgattgta
     6661 ggagacttta acgaagattt ggaagtttat tttccctatc aagcaatgct catgaacaat
     6721 tacctcattg atactgtttc acccggcaaa aataaagggg attttattca gaccgctgta
     6781 catgatttgg ataaaccgca tcttcaatct tacaccgaaa aattaggaca cctattcccc
     6841 attacagcgg atttttccaa aatcacttta aaagattatt ttgctttaat cattccaggt
     6901 ggaagagctg ctgaatatca acgcttaaac aaagatattt tagctatcat cagacatttt
     6961 catgacacaa acaaacccat tgcctgcatt tgtcatggta tacaaattct tgcagaagct
     7021 ggaatacttg aagataaaaa atgtactacc gtcggttttt gtgaaccgga tgttcgaaaa
     7081 gcaggaggac attttatcga cacaggaatg gatggtgttg tggtagatgg taaattagtt
     7141 accggagcaa catggctagg aaatgcaccc tggatgagag catttcttca tatcttgcaa
     7201 aatgagggtt aatcatcttt acagagtaat taaccagtgg taatcagatt aagtcaaaac
     7261 caattcacta tttatataaa aattatattc ttaaaccatg attgttatcg tatcccgctg
     7321 caatggttgt ttgtaaatca attgcagcca cttttttata gaattgcccg gtttctggtt
     7381 gcctttgatt aaaaaaagta tgtcgattaa gtttattgga ctgttttgcg atttttatca
     7441 tagattctaa cgcgctttca gccgttaact ctccttgctt atatttttta taatcatcaa
     7501 atatcatagc agcgccatta ggtatattaa taaccttgcc attttccagc tggcgtgtat
     7561 ttccacccaa taggcctaat tttccatcca gctgaatcac atcctgaata atctcatcaa
     7621 ttaaatcatg agcagttttt atcttctcgt cagatgagtt ttctgacact aattgggcag
     7681 agttcttttc ctgagtgcct tcctctacaa cagtgttctg caagataact tcttgttctt
     7741 taacattaga ctcatgcgct tcaacttctg tttccgctcc agaggctctt gttaaactta
     7801 actcagctac ccttggtttt aatgattcaa gttgagtgga acattgttta atttgtgctc
     7861 ttaacgtgtc atttgaagta actctttgcc aaagctcctg ggcaatctcc tcaacaccag
     7921 gccatttatt tttgcatcgc cattcatttt gtctgggttc aagatccatc aaggcccttc
     7981 caatttgctt taattcttga gccgttggat ttacagggtc agaaacctgg cttaatactt
     8041 tatgaaattt cctgagggga tctgagaatt ctgaaagttt aatagcccct ttaataactg
     8101 gcaatgtcac aggatttaat tttttttcct catcaacata atatagagcc cattccccag
     8161 ttttgtccct gggtctaatc aacaaatagg aatccggttt taactcagag ggtgtttctg
     8221 cagcaataca ccagctgttt tgttttactt ttaattcatt ggcataatga tccaaattaa
     8281 ataaaccagt cttttgaata tcaaaggcta tcagttgaac tgcattttca agtctttctt
     8341 gaaaactcat gttttctatc cccggccttt tgatactggg aacccaactg gcgtcatttt
     8401 tattgttctg tggtacataa aaatagtctg gcgcctccac actcccatcg attaaatacc
     8461 tttgatcttg taattcccaa tgaatcgctt caatttcact tttgtcattc aggatttcca
     8521 ttttttttaa ttgatgaaac aaaaatcctg gcccagtaac acctaatgtt ttagcaattc
     8581 tctcacctct tgtttccttc attgatgcat attcatgagt aaactcaaag gaatctctcc
     8641 tgatataaga ttgctcaatc gcatcagcta attgcctgat tatttgattt cctttgcgac
     8701 ataaaatgat gtcattgcaa agagtattat ttgttccttg agaccaggga gacaatagaa
     8761 acccaaaagg tgcatgaatt tcatcaggga aaggagtaga gcataagata tctgaatcca
     8821 aataaacccc tccctctgca gccaagatgt ttaatcgcaa catatcactc gccatcccat
     8881 aattaggaga ttcctgatcg agttcatgac gtatgctatc ccgacacaca ccttcttcat
     8941 taatatcttt taccgtgatg cctttggatt tcatgtcttg aataaaaaga ccgagttctt
     9001 tggcaggtgc tattttttta tctacccaaa caatcgtttc atagcctggg ttcttttcag
     9061 cccattcaga gacaacctgg atgttttttt gaggcataat atgaccaacc catataaaat
     9121 ggattttttt gtcaatttga gcttcttgac cagcttgtgg taaattttct gtcttcatat
     9181 caattctcca acctggatat gaatacattt tatacagatt gatattaagc ccagatgagc
     9241 atcatgagaa taaaactcat actacatgcc tgttaagaat agccagaacc taacgagagc
     9301 gattaagaaa taaagaataa ctacaagccg atttcccaga tcctaaccca tgagtgtcga
     9361 tggatttccg taacgattat caatataatt ttccgataaa tttgtggctt taatttgcta
     9421 ggaacacagc ccaaaccacc aaggattgaa tcatgaagac cttaatgacc tgcattttct
     9481 ccctcataat attattttca cagcctttga agcctattgt ttttgctcat actaataata
     9541 ccaataatca aggtttatcg gcctcggcta acaacagtgt gggcagcaac ttacctaata
     9601 acatttatgt cacacctcag gctttttgga atttatattt tgatttcacc ggagatgaaa
     9661 ctccaggata tcccaaaggt aaaattaata tttcccaaac cttattccag tcagaaatga
     9721 aaaaaaacag aagcttggcg caacaaaatg aaggtcaatt aatcctgttc atcaactcca
     9781 cactgtatat ttataatagt gaacgtcagc ttgagctaaa acaattgatg cgcactgcgc
     9841 caaattcagg attcactgag atgactgcga tatcacacat tggtccagct ctgatgtatc
     9901 tcgcaaaaat taaagaaaat ggcgatgcaa gctggaaatc acaaatggaa aatttactta
     9961 aggatatcca ggcagtaaaa gtaatcaatg cacaaacacc cagtaattgg ctggagcaag
    10021 tcaatgcacc ggcctggaag cctcatctca ctacaattca taatatgatt gattatgcct
    10081 gttctatggc aggaaattac atgagtgatg tattgaacga aaaactatcg tttgatatgg
    10141 cctctttaca aaacgacttt cttaacggca ataaaacgta tcctattgct tataataacg
    10201 tcatgatcgg cacttttatg ttaaccgcct tgcaaagcat ggatcaactt catagtaaaa
    10261 taagccaatt aaaaatagat tggcctcatg cgaaggtaat tatccgtttt gtcgcaggat
    10321 ctaatgtttc agctggcgtg actaaaggca gcaattggtt ggtcccattt gttcaagcac
    10381 tatccaataa tacattagct acagatagaa tttatatcac gccttatgcc gcagtgaaac
    10441 catcgttagg cgcacaagag ttaacacaag cagattacaa ctattataat aatacggtat
    10501 gggacgcaag acataaccgt agaatcatcg ccaacgaagt ctttactaac atcacgagca
    10561 tcttcttacc agatcgacct gctattcctg gtgattatac ttattccaaa ccacctaaaa
    10621 tcgaagattt tctaatgcgc ttaaaatttt cattggcaga accgactgaa atgttatcca
    10681 atacagttgg gttttggatg gctggtgaac ttgccgaaaa aaactggaat tacaataaaa
    10741 tcagcatccc gggtattact actggtttcc cacaaggcat atccacctat ccgaacaata
    10801 atcctgtaat tcagcgatga acaaagcaat caaattatcc tcttaaggtt tgattaatat
    10861 tcttacatta taattactca atataaccct tgagtgagga tatcaaatga aagattttgt
    10921 cattgttggg gctggaccaa taggcttata ttgggctgca agacttgcac aaataaaaga
    10981 aagttcggga ttacctatca atattgtagt tatcgaccca agagcgggca attacaatcg
    11041 agaaaggata gtatccaatg aagcaatcaa atcgataagc tcccgattct caatcagtat
    11101 gccgtccggc aaagggatcc cgagtgaggc catgtatatc agggactttg aaaaatcact
    11161 atacgaatat tgcgaaagta agggtgtaac atttaaaaaa gggacttttt ccggcctacg
    11221 cgacaatttg gtttcgtata aagataataa gcaaaacatc cgtacagtca aatgtgacat
    11281 ggtagttgat tgttctggtt ctaatcgtat ggttctcaaa gaaactaatc gcttgctggg
    11341 aaaagaagta tttgaaatca agacattggg taaaaaccca cataccaatc atttttcttg
    11401 ctatatgtca ttaagcactg aagaagcgaa gaaactactc atcccagacg aaatagatcc
    11461 tttgcttcag gcaaaacaat tatgcaaatt acgacaaaaa tatgattggc caaatttttc
    11521 atttccagtg gtcgacatac gctctgcaaa aaatgaagaa ggtggcgtta attattttat
    11581 ttattatgaa gtacccgata atttgaaaga gaaagacaag agtgtacaaa ttgaatttct
    11641 taaagattta ctcaaattga aatacggttt tgaagacatt gattttacaa ttcattcgtt
    11701 tggacatttc cctgtttgtc ccaagtatgt cgataagcca ttttatcttg gtgaagactt
    11761 acctgttgtt ctggcaggcg gtgattgtca aattgaaccc gattatcgta aaggaattgg
    11821 catagaaagt ggaattgaac gtgccaattt tctatttgat actgtacatg gtacaagtaa
    11881 aggactgggg ttcttatttg ataattacta tcaacaagta gctagatatg taggctatca
    11941 tggcaacctg attgaacagt tttatctgca aagagtggat aacattaaag gctcttcgct
    12001 tgagcaagca aagaaaattc tttgctctgc ttgcgggtca gttaaagaaa tcgaagatgt
    12061 tgccgccatt gctagcgaat taaagctttt aggcaatgaa cttttcaaga aacctaatta
    12121 tgaaagtgct ttggaatgct atcttaatgc tatccatttg tatcaatcct ttgaaaaagc
    12181 cttaccactg acaatggatt ttgtgacatt gcattccaat gcctgccaaa cctgcctgaa
    12241 gttaaaaaaa tatgaacaat gcataaatct ggcaaatgaa ggcatcaagg cttatgcgga
    12301 aataaaagca gaggataaag agatgctttt caaattatta ttccgaaaag cctcagccct
    12361 cgttgaacta ggtaatgctt ttgatgtcaa gacacaaaga aaagagttcg atgaggcatt
    12421 gaaagatctg aaagaaacat atgagctaat gcaggaacac tcaggtgtga ataataccgc
    12481 ttttgttaag cagattcaga ccaaaattgt aactatcgag aaaaaacttc ctcctccaca
    12541 agaggaaata aataaaatcg aatttattta gctttaaatt ggtaacatta taaaaatgcc
    12601 tggcttgctc attaatggat agaaatctat ctgacaatga acaagcctaa tcattatgac
    12661 tctcttcccc aataaatgtt caataaagct caatgctgct attcttaatt gaaatagggt
    12721 tgataaggga gtgaaaatgc taaacaaggg atatattaaa acaacaccct atggattggg
    12781 attttcagaa cttttttttg aagacaactt aaaaaaatat ttttctgatc cttcactaga
    12841 agtattgtca gcagaatact ctccttctgc cagtgataaa atggcatggt ctgccaaaca
    12901 ccccaaacag ggggaccacc ccataggagt acaacgttat aaaataagct ttaaaaaaaa
    12961 cggtaaaata caaacccgcc atgtcatcgt caaatctaaa attagcgata cgcattatct
    13021 ccaggttata tccaatgcct ttacaaaatg cggtattcaa acggaaatgc ctatccttga
    13081 ctatttatca caactggaat tttcaaattt aaacaggaaa gaaatcacta tttacaatat
    13141 gcaacattca tttgaagcct ttcaatatgc tatgccatta tgctatggaa cttatttaga
    13201 cgaaaaagaa gaagcttgtg tattaatttt agagtgcctt gatgaagaat ggctatccct
    13261 cgatcctttt aatacctcga aatgggatca aacagccatt gaagcgttga ttgatgcgat
    13321 tgctccgatg cacgctgtct ggtataaaaa agaaaaaacg attgctgaga tcaaaggctt
    13381 caaaaatccg ttaaataccg accaaatgca acgcttcata ccttattgga atgccctgga
    13441 aaaagccgtg aaaacatcaa acttgccttt tcttacaaag gaagattatt tattccatac
    13501 tcatcttatt gaaactatgc cgcaatggca tatcaaaatt gatgctatgg ctaaaacatt
    13561 ggtacaaaat gactgtgtac cgaaaaatat tgcattatgt aatcaaaacg gggcaaaaaa
    13621 agttttggtt tatgattggg aaattgcaac tattcatata ccacaacgcg atattgttga
    13681 gtttttgtct tatgtcttac ctgaaaattt cgatagtacg ttattaggca attatataga
    13741 gagacatcgg gtaaaacttg gcgaaacaat aaaacagccg attgatgcaa cagagtggcg
    13801 actcggattt caatatgctt tatacgatta tttaatacag cgtgtttttc cacaaatacc
    13861 ttttgaaaag cttgaagctc gtaacatagc aaaagtgtat aacaatgccc gtcgcatgat
    13921 ggccctactg aactaatttt ctggagtgat taaggatgac tcatatacgt gtaatactac
    13981 ccgggtacaa tactaaagtt ttaaacaagg aaatgttgaa accatttgaa agccctggcc
    14041 tggagttaag cgttgattat gctgacattg cggttgatgc ggtaaggcac gaatttgata
    14101 ttgccttgat ggcgccttat ctcataaaaa aagtaataaa agccgagcaa gaaaaggttc
    14161 atgccatagt gattgatatg atggcagatg tggcaatgcg cgcacttcgc gaggcagtga
    14221 caattcctgt cgtcagtctt gctgaaacag caacacatgt tgcagccatg atgggcaata
    14281 attttgcgat tatcaataca acagatgaac tgacgcccat tcaggaaagt ttggtccgta
    14341 gttatgggtt atatgatcgg ttggcatgta tccaaggcat acaattggat ccgcatgacg
    14401 attatcgctc agaaaaaata atccatggct tagttgagca atgtgcggat gccgtgataa
    14461 agaagcgtgc tgatacactt attttcgggt cagggcgttt gattggttat cgtgaagtta
    14521 ttcaaaaagg tttagaggaa aaagggttac acatcaccat tattgagccc ctgcctaccg
    14581 gaatttattt tgccaaattt ctggtagact ccaaaatttc ccaaagcaaa tatgtttatc
    14641 ggtcacccga tgcttccacg ttaaaaacgt attcaaaaat tttgggggat taatcatctt
    14701 gattttatct cgaatatcat taggcctggt gattactgat aaactttctt tccatcaata
    14761 taaacagcaa ctaacttgtt ttcgcaggat ctggcaatca ctcttgccag acgatattga
    14821 agttcatcaa gggctaactc ttgtgacaaa gaagaaagct ctgccagagg atagttattc
    14881 ctgtcatcca ggataatcaa atccgccttt ttacctgctt cgatgctgcc aatttgcttc
    14941 tctaacccca gcaatttagc tcctccaaga gtggcatgat atagccagct ttttgcatca
    15001 atcagagccg tctttttatc aagcctccat aatgctttat tagtaaaact cgctgagcgc
    15061 atttcatcaa aaagagaaag actgtcccct ccaccactgt ctgagcccaa tccaacctca
    15121 atccccaaac ctttaaaaaa agaaaccggt gccattccca tagcaaaaga aaaattacta
    15181 gagggacaat ggctaattcc aacttgccca gcatagtccc ccatgcgttt catttcgtga
    15241 agtgataaca aagtgccatg tgcaaacaca gtcttatcag caatacattg tgtggattca
    15301 aatacatcaa aataatcatg agctgtgcta aacaagcttt gaatcaattc atcaaaccct
    15361 tcatccttat caagatgagt atgcaacaac agcttgttag cacgggcgaa ctcacccgct
    15421 tgctttaaca tagtcgcaga acaagtcacc gcaaaccggg gagaaactgt gatatgtaga
    15481 ttatttttac cgtggtaacg atcttttaaa acagtcagat ctttaaatgc ctgatcacaa
    15541 tcgacaagca aatgatcagg tgcgtttcta tccatcaaca ctaatcctat aaacgctctg
    15601 ataccaatgc gcgcagccac atcacaaact atttctgtcg cttggcgact gctggttacc
    15661 atcgtattga tagtcgttgt gccaaaacct aaacaagcat gcatataaag ctcagcgtag
    15721 cgttgacatc tttgcaggtt acttgagaaa gctgcttctg ctggaaaaac caaagtgttt
    15781 aaccaatcca tcaatggcaa atcaccagct ccacaaatag cgtattggga aagatggctg
    15841 tgtgtatcta ttaagccagg gaggattaaa tgatcaggat aatttgacac cgtataggat
    15901 atttcttgat ttaaatgagc ctgctcaccc acctcaataa tgctatcacc atcaatcaag
    15961 acataacctt tcttgataag aagactctgc cccgcttgtg taggagtgat aatgtcccct
    16021 aaaatcagat gaggaatgct attcaattca tatcctgaga tttatttctt actatcagaa
    16081 agtatagaca accccctgga ttaactgaaa tttggagtag aaaatccaat agtaaaagag
    16141 ctccttcttt tcaatttgga tatgaaggag ctcttttgta agatcaataa cctaaattat
    16201 tcatctaatg agcttttatt ggcctctcat aggtaaaatc ctgctctctc gctcagctgt
    16261 tctttctttt gaaattttgt ccatatgctg atgagctacc tcttcatttc ctttgccaac
    16321 ccgtgaaaaa cgcagcgaaa caccacccgg gattaaactg cgattaaaga ataatccacg
    16381 gtaatctgtt tctgagtagg ctttaggccg attgacattg aactcaaaag acaagttctg
    16441 caatgcttta acaaccattg atttagcagc attagccaag tgctcttgca tttccggagt
    16501 aaaattctca ctgtaccaat ccaaaggttt tggcacatca atatcaataa atttttctgg
    16561 gtgagattgg gcttttaatt cttcaatcat atttttcaag acaaaatcat gtttggagac
    16621 aattttttgc tgacaaaaat tgcgttgaaa caaccccggg cctgtaccat catttgtttt
    16681 acgctcaata atggttagtc cttttttccg taaagcaaga ccatcaaact cctgtctttc
    16741 accactttct ggagaaataa cctcaaagga tttgtaaagg cccactgcca accgctcgga
    16801 aaaatccttg atagtcaaat acaactgcgg atcactggtt ttcaggctag acgattgaat
    16861 agattgcatc aaaccctcag caagatgact tggaccactc ataacctgcg gatcagaatc
    16921 acctgctttt ccttccagtt tttcctttcc tttctcacga tcattgattg gagactctag
    16981 taaactctct tcatcatcaa agattttctt gcgcaaatca tcgagggttt cttctccatc
    17041 agaatcataa tcaatcaact ctaccccttc cacggttgca ccctcttgag gaggataata
    17101 aattaatgga gacacagttt tgaaattttc aagattattc aaccagtctt gcaatatttt
    17161 atccattcct tcaaattgac ccaactctgc catagcgcgt aaatatcgct cttgataatt
    17221 acgcgattca gaggctgaag ctgtattctc tttctcttga cttttgccgt ctatttcttt
    17281 acttctctct ttcacaatca ctcttaatgc ttccagatac tcaatcatag gaggaattgc
    17341 cttttttctt tcgctgacaa aatgttgcct ggctgcttca attcgttttt tatagtcctc
    17401 ttgtaattcc tgctttctgt tagcccatac acctgttagt tcctcccgga tagcctttgt
    17461 ttgggtcttg cctttcatca tgatagagtt cgcctcttcg acaaactgtt tatagtagtt
    17521 tgcataagga gtattgcctt tcggatgtgc gcttctcaga agatgcagtt gttgtttcat
    17581 ttcatggaat tcctgggcat agagttttct ttccagttgc agacttttta gtttattttt
    17641 cagttgctta attacgatct catcatcact gccaaattca gaaattagct gcggaattct
    17701 tttgatttca ttatcaatat tgttcaacct tctctcataa aaccctagaa tttcgctgtg
    17761 ttttttcaaa tcaaaaacat cactgggatt taaagtttga aggtgcttaa ggcaatgact
    17821 cataacataa gagtaggctt taaataaatc accattacta ttggtttgcg gatctgtcga
    17881 aaaatcaata tcctcatctt cccgtaaact atcacgacgt ctatcttcaa ggaatttgag
    17941 ttcttttagt gctcgacgaa gccctcttaa ttgatacggg atgttcttag tttcaggtaa
    18001 aagtgtacga accccttctg tagttctagc tacttcatca atctctttaa tggtcttgtg
    18061 caaatcatca ctcgattgag ttttcttcaa tttatcaatc aattgcccaa ttactttatc
    18121 caaaagacca caaacaactt gctcttctgc caatttttca ttctttaaaa gatcagcata
    18181 cagtgttttg aaatggggtg ttaccttgct atttctagca gggttatctg atgcaattag
    18241 aacaggattg tcaagattaa actgacgagt taaaagttct actccacccc ttactataac
    18301 gtcgttccaa tcatcattaa gatttggagg ctctttgact atcactttat aaccagcaaa
    18361 cactaatgct ttgcaagcct tttcaaattc taaattagcc agtggattcg ctacaggatc
    18421 atcatgatct ttcagtaaaa caaccgttgc cccgggagga taatgagttt tgacataagc
    18481 caaggtggag ggtagttgag atacacccat tgacgccaat accgaatagt tatttctgac
    18541 ctcatccaga ctcgccacac tggccgctgt ttcaactcct tcggcaataa aaacaacatc
    18601 gacgttatct gttttattaa taacagctgc tttgccttct cgcagtggat tcgcctgtcc
    18661 taaatatctc ttgcaaaaaa aatgtgaact ttcagggttt gttgctttgt tgccatcggg
    18721 gtcaatttgt attatctgta gaccaacgag ttcatcatcc agattataaa caggcgcaac
    18781 cacataatct ttataaggtt tgtttttatc aaactccgga ataccaatag gaccttttaa
    18841 gcttcttatc tgtaattgtt cagccacacc tgctgggatt tttctagttc ctacgatata
    18901 acgttcagca ggggtacctt ttaaaggtac gcttcttgcc cataaatcat tggctaattt
    18961 aatgcttttt ttatcgatga catatttatt caactgatta atttctggag ttgttgtctt
    19021 gcccatacct attacctcac ttattacctc acttaaatga agtgaaaatt ttgacatatt
    19081 tagcttaagg aaatattaaa aacaacacaa aaagtatatt aattagtcaa atattaccca
    19141 ttgatagcaa aaggagtagt tacttgtctg ataacaaatt agaatagccg ataatgcaca
    19201 ttatgaacaa agcatagata gagagtaatt gaatatcttt ctgactctga tcactcaaag
    19261 gtctttcagg atcaatatct ttatccaaac tgtcaccatg ttgaacaaga taaattttca
    19321 tctcgccctt ttaattcatt agctaattta ttaaagtgta gactcatttg aaataacaga
    19381 taagaaaagc ttgtaatgaa acctaaaatt tcagtattta ttgcaacaag tctcgacggt
    19441 tatattgcaa ggaatgatgg ctcaatcgat tggcttatag aagcaaattc tcttgcctct
    19501 cctggagagg attgtggata tcgatcattt attgccacca tagatacgat aattatgggt
    19561 cgatattctt atgaaaaagt tattacattt gatgaatggc catatggttc cataccggta
    19621 atagtactga gctcaaaacc catacagata ccagaacact tgcggcatat cattacagtt
    19681 tctaaccaaa gtccccctga tttagttagc gaattatccc tccaaggcat tcagcatgta
    19741 tacctggatg gaggtattac tatccaggga tttcttcaaa ataatcttgt taatgagatg
    19801 accattacag taatacctgt tttgattggt tcaggccgct ctttatttgg ctctcttaag
    19861 aatgatatca aactcacaca ccagttaact actagctatc caggtggctt tgtccaaatt
    19921 aaatatcaga ttagtgcata accagatact ttaataatgt ttacttcgta attgtaatgc
    19981 aacacgcatt tgctatgtta tgatggcgta atgccaattt actactaaca ggataagaaa
    20041 tggatattcg ctatttgatc tatgcttggg gtagcagcat tttattcttt atttatcttt
    20101 atttaagtta tctggttatt atccaacgtc gtaaatttca tgtaccgtat ttaaccaatg
    20161 atgataaggc atttattgct cgctttcgcg cgcatgccaa ctttgctgaa tacgttccat
    20221 ttgccttaat ccttatgggg atatccatta tgttccaaat ccctccattg ctattggcta
    20281 ttttaatgtt tgctctgata atagcacgct tgggacatgc ttatggtttg attaaaaaag
    20341 aagcgaaaaa acaatttaaa tttcgcattt attcggttgc aacaacattc attatcatgg
    20401 gaattttagc tatttttaat gtaagtaaag cgacgatact atatataggt atctaattat
    20461 tgagatgatt ttaggttttt tggcgatttg gcctctataa tttgcgaacg aaacgatgga
    20521 gatactcatc aaatacatat tgacttactc ttgctacaga agccttaccc tactgaataa
    20581 ttctcattga gccgcatcac aacatattaa ttcattaagg aatcgtattg tttaagaaaa
    20641 ccaggggctc attatcgtcc caatcatcct cgctactact attgccatag ctccaattat
    20701 cccatttatc gcctatagaa aactcctctt taaactcatc atatcgatta tttaatagag
    20761 aatatacttg ctcaaaccat tcatacatct cgctgaacag atcgttgtaa atatcaacct
    20821 caatatcttt aatcaggcct atcttgtcat tgaaatcttc caggtgatta tgtagatgct
    20881 taaccacaaa tttgtagtgc tcaaggttgt atttatcatg attcaacaga atatggctta
    20941 tcataaattt tgtttttaat ctcaatacat ctacttttag ttgacaaatt tgcttgctca
    21001 aatcttgctt atcctttttg aataagaaga accaattaaa aaggacatac tttaacctga
    21061 gctccagttc ttttatttct acttgcaacg ccgaattgaa atctttatct ttgagtagtt
    21121 cactataaga ggactcaata tggtttaaat ccttctcgaa ttctttctta accgattggt
    21181 aaatactcat acttttctta tcatacggat tgatagcaaa aaattttgtg taagccacta
    21241 aaactc
//
LOCUS       DBNCWK010000043        21217 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.043, whole genome shotgun sequence.
ACCESSION   DBNCWK010000043 DBNCWK010000000
VERSION     DBNCWK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21217
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.043"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..576
                     /locus_tag="AC06IO_001412"
     CDS             <1..576
                     /locus_tag="AC06IO_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2271 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874610.1"
                     /translation="FNGQELSIKFLQQLNQIKYFYNSAFNKTENEDDGLEVVEEYENF
                     ISLVSQVKSGQVNADKAFETIKDTTESRQADVIIANFFKVCELMFWATAAFFSYVACV
                     SVGIPLTFCDPLLGIAVSLSTFLMAISTASKFLDCFDEFQSLDKINEHDQNQKDTVRF
                     FSKTSNSTSSVRNKEAEEESSQRLYPNLQTV"
     gene            complement(750..1238)
                     /locus_tag="AC06IO_001413"
     CDS             complement(750..1238)
                     /locus_tag="AC06IO_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 family protein"
                     /protein_id="HIO9874611.1"
                     /translation="MIIRFSKVLLVVAVSFFCLLTAFGNITDYSANFPAVVKVLTMSD
                     IFPDSTITYRAITSPVLHYVAFIIIVILELLTAIFCGIGAWKLFKARNESASVFNHSK
                     NWAIGGLTLGFVTWQVIFMSIGGEWFGMWMSPTLNSALTTAFHIFITILAVMIYLVIK
                     DE"
     gene            complement(1383..2696)
                     /locus_tag="AC06IO_001414"
     CDS             complement(1383..2696)
                     /locus_tag="AC06IO_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIO9874612.1"
                     /translation="MKILFLLLLVFLSVIPAQAKPVELVFWHAMAGHLGDEVRLLADD
                     FNKSQNQYRVKPIYKGNYTETLTNFAAAFRARQAPSIVQIFEVGTSIMLAPPGVIKPV
                     DLLMSEQGISLPKDDFIQSVREFYSRDGQLMAMPFNLSAPVLYYNADILAKVGYGKHN
                     FPQTWSAMEIMAEKIKKAGYDCTYTTAYPGWVLFESFLAIHGLPITQGNPARAAFHTP
                     QLMAHFQRLKRWHDLHYFRYGGRVDDATILFTSSVCPLFSQSSGAYNSLSALVPFHLG
                     VATMPLDTGASPIRYANVAGGAALWAVGGQTEEQYRGIARFFAFIAKPEVQKRWHEHT
                     GYLPLGLKGIYASIVQSSQHPALLLARTDLEDNLPEKPYKHMGPQNQIRGINDEVLEA
                     MFADLMSPEEALNEATIRANHLLLRFARNTQREGVIDSKVGKGLG"
     gene            complement(2693..3412)
                     /locus_tag="AC06IO_001415"
     CDS             complement(2693..3412)
                     /locus_tag="AC06IO_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HIO9874613.1"
                     /translation="MILEKIIGHRGASGYSPENTMASFNKALSLGCRFIEFDVMCSLD
                     GEPFIIHDDNLKRTTNGKGEVGLVEADYLRGLDAGSWYSKQYKGEKIPHFTDVLKWLS
                     FSDVQANIEIKPYPGTVEQTTVSVLSHIHRHWPQNKELPLVSCFEWDALVLCRSIAPE
                     MPLGLLLHEWDKDWLQKAKQLECYSIHFNRKILTADRVKAVKDQGYVVCAYTVNRKRL
                     AKKLFDWGVDALFSDYPDLLK"
     gene            complement(3417..4112)
                     /locus_tag="AC06IO_001416"
     CDS             complement(3417..4112)
                     /locus_tag="AC06IO_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874614.1"
                     /translation="MRKIAWMLGLMVLTPFVWADNETPDLVLDKIFFQVSAKKWVTTQ
                     TALLNVSINATLSNADLVKARADIMDSLNKIAKGEWHLVQFDRSQDSSGLEKLYVQAQ
                     ARVDQSALTNIYQNAKSVSKPGAKYEISGVEFKPSLEETQVVRAKIREQLYQQVNDEI
                     DRMNKAYPKQNYSVSNLVFFEGDNIPQQPRAYQAKEMNALAMAAAPAPLTVSNELILT
                     AMVEAASNRKQGD"
     gene            4675..5748
                     /locus_tag="AC06IO_001417"
     CDS             4675..5748
                     /locus_tag="AC06IO_001417"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Leu/Phe/Val dehydrogenase"
                     /protein_id="HIO9874615.1"
                     /translation="MMSVDNIKNDNQVLAEEDFLEYALSHGFGDLHFKVDPQTGMKAM
                     IAIHSTKLGPALGGCRFIEYPNTAAALKDAMRLARGMSFKAASVNLPLGGGKSVIIKP
                     KGFFNRAEYMHRFGEFVNELNGRYITALDSGTELSDMDIIAEHTDYVASLSRYNGDPS
                     PSTSKGVLRGIQAAVAFKLGKDSLKGLHIAIQGLGHVGYALAKQLHELGATLTVADIS
                     PAAVNMAVQELGAKAVGTDIIHKVPCDVFAPCALGAIINDLTIPQLQTSIIAGAANNQ
                     LAHTYHGKILHEKGILFAVDYVINAGGLIFAASKYLQTPEDKVSEQIDSIYTSLTEIF
                     TRSAKDNLPTSEIADTLAKEKLA"
     gene            5763..6419
                     /locus_tag="AC06IO_001418"
     CDS             5763..6419
                     /locus_tag="AC06IO_001418"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9874616.1"
                     /translation="MKHLSLTPELYKYLLDISLREHPALAALRKETSTMELANMQVAP
                     EQAQFMQMLIRLIRAKKVLELGTFTGYSALAMSLALPDDGQVITCDINEGWTKHAHPY
                     WREAKQEHKIKLRLGPALDTLHSLLNEGGEHQFDFIFIDADKTNYLNYYELALKLVTP
                     KGLIAIDNIFWDGKVIDPSDTSGQTREIKKLNQVIKNDSRVFVSLLAIADGMFLVQPI
                     "
     gene            6486..7532
                     /gene="hppD"
                     /locus_tag="AC06IO_001419"
     CDS             6486..7532
                     /gene="hppD"
                     /locus_tag="AC06IO_001419"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIO9874617.1"
                     /translation="MQNNNPCGLDGFAFLEFSGPDRNKLHQQFSEMGFQAVAHHKNQD
                     ITLFKQGEIQFIVNAASHCQAEAHASTHGPGACAMGFKVKDAKAAFQHAIAHGGIAFQ
                     DAPHANHGLPAIQAIGGSVIYFVDEEHQPFSHEWNITSPEPVVGNGLTAIDHLTHNVY
                     RGNMDKWASFYASIFNFQEIRFFNIKGKMTGLVSRALGSPCGKIKIPLNESKDDLSQI
                     EEFLHEYHGEGIQHIALNTNDIYKTVNGLRKQGVKFLDVPDTYYEMINDRLPWHQEPL
                     NQLHAEKILIDGEADPKDGLLLQIFTENIFGPVFFEIIQRKGNQGFGEGNFQALFEAI
                     ERDQVRRGTLKELT"
     gene            7588..8586
                     /locus_tag="AC06IO_001420"
     CDS             7588..8586
                     /locus_tag="AC06IO_001420"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIO9874618.1"
                     /translation="MKLASLKSTRSRDGELCIVNRLLTAAIRVPHIASTLQYALDHWD
                     VVEPKLQLVYQQLNDNQIEDAFAFAPELCASPLPRAYQWADGSAYVNHVELVRKARGA
                     EMPADFWTNPLMYQGGSDAFLGPRDPILVSSEAYGIDFESEVAVITDDVPMGIDHTNA
                     AHHIKLLMLVNDVSLRNLIPEELAKGFGFFQSKPASSFSPVAITPDELGSYWDGQRVN
                     LPLISHFNGQLFGQPNAGIDMTFSFPELIQHAAKTRFLTAGTIIGSGTVSNLDRSKGS
                     SCIAEKRMLEMIEFGQAKTPFMRFGDRIHIEMLDGQGYSLFGSIDQIVTKYEKDSL"
     gene            8583..9221
                     /gene="maiA"
                     /locus_tag="AC06IO_001421"
     CDS             8583..9221
                     /gene="maiA"
                     /locus_tag="AC06IO_001421"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HIO9874619.1"
                     /translation="MILYDYFRSTACYRVRIALNLKKIVYEKIEVHLVNNGGEQHSLQ
                     YHQINPQELVPSLDINGQILSQSMAIIDYLEEIYPEVPLLPKDPFMKANLKSLAFIVA
                     CDMHPLNNLRVLNRLKEQFKASQEQVMEWYHHWLKTGFDAFEEKLRAMKRDKPVCFGN
                     EVSLADVCLIPQVYNAQRFHFDMASYPIINQINEYCLTLPAFHDAAPEAISS"
     gene            complement(9218..9778)
                     /locus_tag="AC06IO_001422"
     CDS             complement(9218..9778)
                     /locus_tag="AC06IO_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874620.1"
                     /translation="MLQRVLLVLLCFFSFLASASDTVLKLYRPFGDVFEQAPPVVKKI
                     MSGQCYEQSHIIIREDAWRCQAGDQLFDPCFVKAGSKKMEAVCPQSPWVGDSVQINVG
                     IPLNNEHHKTLDMSRAFPWAIELVNGEYCLAVNSNEQYDSMPVRYRCSNQNVLIGYLQ
                     RCKTAWSMLEKTPKGVITVELRRAWF"
     gene            9926..11329
                     /gene="asnS"
                     /locus_tag="AC06IO_001423"
     CDS             9926..11329
                     /gene="asnS"
                     /locus_tag="AC06IO_001423"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIO9874621.1"
                     /translation="MTEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHD
                     GSCFSPIQIVATDQLSNYHKEVTKLTAGCSMIATGKLVASQGKGQFFEIQAESIEVVG
                     WVENPDTYPIQAKRHTLEFLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFW
                     VHTPIITASDCEGAGEMFRVSTLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVE
                     AYCMAMSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFANLEDICKLSQNMLRYLCK
                     TVLEERSDDMDFFNQFVAPGCIERMEHIADSEFEIMTYTDAIKALEASEQKFEFPVSW
                     GLDLQSEHERYLAEVLCKKPVIVTNYPQEIKGFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEILDRRMDECNLNKEHYQWYRDLRRYGTVPHAGFGLGFERLISYVTGVS
                     NVRDVIPFPRTPGHADY"
     gene            complement(11326..11724)
                     /locus_tag="AC06IO_001424"
     CDS             complement(11326..11724)
                     /locus_tag="AC06IO_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874622.1"
                     /translation="MDEKKDKTIQINEITSTVEDEKKAQPLRKSERNLPVKSHRRSFS
                     IFYPYSTENSKISSSTQTSNKGFLPGPDFNREHYDTVDAGGPLASNADDEILEKIFSP
                     RNRRRLLSEGNQPQQQTAEVEEDSLSALSI"
     gene            complement(11748..12431)
                     /gene="lolD"
                     /locus_tag="AC06IO_001425"
     CDS             complement(11748..12431)
                     /gene="lolD"
                     /locus_tag="AC06IO_001425"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HIO9874623.1"
                     /translation="MNDTILTSQKLYKSYHDGTSTVEVLKGVDLAITKGDRIAIIGPS
                     GSGKSTLLHLLGGLDKPTSGLITLGNVNWQKINEKQRCQLRNQQLGFVYQFHHLLPEF
                     TALENVMMPLLLAGMAVKDAEEKAINMLEQVGLKPRLTHKPAQLSGGERQRVAIARAL
                     VHQPHCVLADEPTGNLDEATASKVFDLMLELNKKMNTALVIVTHDQRIAERMDRVLVL
                     HEGSLYARE"
     gene            complement(12424..13671)
                     /locus_tag="AC06IO_001426"
     CDS             complement(12424..13671)
                     /locus_tag="AC06IO_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIO9874624.1"
                     /translation="MYKPLALFIGLRYTRSRKKNHFVSFISLSSMLGIGMGVMVLITV
                     LSVMNGFDQEIHRRFFGMAPEITITGPDERLSDWPEVVKKVETIPGIKAIAPYVGSQG
                     LLTHEGQVLPIVLTGILPEKEQSVTHLNKKLLAGNMDNLKHFGIILGKGLADSLGVMI
                     GDKVTIMIPQATVTPAGMIPRFKRFTVVGVFSAGTGFNFDTKLAFINIEDAQKLMQMD
                     KNDVSGIKMKINNVYKAPELSYELSDLLGEGYQVGNWTQQFGAFFEAVKMEKTMMFMI
                     LLLIIAVAAFNLVSSLVMVVNDKQAEIAILRTIGATPSTILWVFIVQGMMVGLVGTIL
                     GLLGGLVLANNATEIVNALQSFFQVKVLSSSIYFVDYLPSKIMFRDLWQVCAMALSMS
                     FAATIYPAWRASKTVIAEALHYE"
     gene            complement(14112..14198)
                     /locus_tag="AC06IO_001427"
     tRNA            complement(14112..14198)
                     /locus_tag="AC06IO_001427"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14162..14164),aa:Leu,seq:taa)
     gene            complement(14217..14290)
                     /locus_tag="AC06IO_001428"
     tRNA            complement(14217..14290)
                     /locus_tag="AC06IO_001428"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14256..14258),aa:Cys,seq:gca)
     gene            complement(14351..14426)
                     /locus_tag="AC06IO_001429"
     tRNA            complement(14351..14426)
                     /locus_tag="AC06IO_001429"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14391..14393),aa:Gly,seq:gcc)
     gene            complement(14479..15042)
                     /gene="pgsA"
                     /locus_tag="AC06IO_001430"
     CDS             complement(14479..15042)
                     /gene="pgsA"
                     /locus_tag="AC06IO_001430"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIO9874625.1"
                     /translation="MSTLTSLPNILTLMRISIIPVFIMVFYFPFKWAHPTAAVLFAIA
                     SITDWLDGYLARKLEMMSHFGAFLDPVADKLLVSTSLLLLVGAKDIDYITIPAIVIVG
                     REIVISALREWMAEVGSRASVAVGYIGKVKTSLQMIALVLLLAFNPLVSWWGSLGFIL
                     LYVSAILTIWSMVIYLAIAWPELMKKS"
     gene            complement(15063..15722)
                     /locus_tag="AC06IO_001431"
     CDS             complement(15063..15722)
                     /locus_tag="AC06IO_001431"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIO9874626.1"
                     /translation="MSSAYQLVIFDWEGTIADTLGVILHTVATEAKMLGFGDIDPYQA
                     RKYVDLGLVQAVKKTLPNLTAGQQEDLLQAVQSAMISRPTEVRLIPGVLEFIQQLHQA
                     KVDLAIATNKGHNSLIRALQATELDQLFKVTRSAGQVPAKPCPQMLEEIMEEFGRDPS
                     STLMIGDSTTDMEMAKRINVNAIGMDFYHQQEDALKAAGALAVFDDYKLLSAYLMLPE
                     A"
     gene            complement(15715..16662)
                     /locus_tag="AC06IO_001432"
     CDS             complement(15715..16662)
                     /locus_tag="AC06IO_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIO9874627.1"
                     /translation="MSEVSYKEIGSEEDGQRLDNYLIRILKGVPKSHIYRIIRGGEVR
                     INKKRAQANSRLQIGDSIRIPPIRMTDSKEIFVSDNLAKRLKDSIIFEDSSLLVINKP
                     AGIAVHGGSGLSLGVIEALRKTRHDLSYLELVHRLDRETSGCLLLAKKRSTLRAIQAL
                     LEAREVQKIYWALLTHRWDGKKKITITAPLEKNTLKSGERVVSVQKDGKASETDFKLL
                     ENYHQACWVEASPKTGRTHQIRVHSAYFGHVIVGDEKYGSKVGEVEGIDYQQNRLYLH
                     ARSIQFNLNGVMHVFQADVDDGFGDTLKQLRARSLLSYE"
     gene            17562..19553
                     /locus_tag="AC06IO_001433"
     CDS             17562..19553
                     /locus_tag="AC06IO_001433"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIO9874628.1"
                     /translation="MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKA
                     IVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQEL
                     LIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNAL
                     TLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIR
                     SIRDNLRKSISEIIIDDQISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQI
                     ETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIEATALNTNLEAADEIARQ
                     LRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQR
                     LRLSLGETAQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMAT
                     FIMNEKRNFIRDIEKRHGVSVVVIANPYMHPPQYTITRLKEDNVGKSKKPSYSLIQQP
                     EVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSVAHTVTQEPG
                     KKHHRAQNQGKHPSSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRK
                     KSNANQQSHGSQQSNRSIPAKNEGNKKREAILAKEQEEK"
     gene            complement(19917..21194)
                     /gene="legC7"
                     /locus_tag="AC06IO_001434"
     CDS             complement(19917..21194)
                     /gene="legC7"
                     /locus_tag="AC06IO_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC7/YlfA"
                     /protein_id="HIO9874629.1"
                     /translation="MATNETELQVLIQHDSKSTITTSSLDSTDKDPKTSTGTPEIESA
                     SLAQIVDTQKQLSQVKESLESIVDSIAENPSLITRAASAWGELPMWQKVTGGLVLTAP
                     TLAVGLFAHIGVLLVIGGVTGLTYTAGAIVLDDHHTCNVNIAKRLKEGLFGLADLLQI
                     TIEALDAIRKKFAEEIEKFKNENLRLTDNIDRLGNEVESLSAQVELYMEIEKMLRKDT
                     NEMEQTVKKLQESTTKQTDLLEKNQKELSKIRKEYEKSQLQLAEKIAELHEVRLSLGL
                     EVQKAKTVAKTLEGTVQTLTGTVIADEEQRVSFQKKLDGFLNDKQLSFDQVAERICKA
                     EEELKKVKEELRQSNDRYSELLKRQEQQVERLEKLGLHKLERIVDKENVKPSNDPVHS
                     GLLSHGIYSTPKGKVTQPKVEVVEDNKTIALVN"
BASE COUNT         6545 a         4508 c         3985 g         6179 t
ORIGIN      
        1 tttaatggcc aggaattatc aataaaattt ctccaacaat taaatcaaat caagtatttt
       61 tataattcag ctttcaacaa gaccgaaaat gaagacgatg gcctcgaagt cgttgaggag
      121 tacgaaaatt tcatttcgtt ggtctcacag gtaaaatctg ggcaagttaa tgctgacaaa
      181 gcgtttgaaa caataaaaga tacgacagaa tccaggcaag cagacgttat tattgctaat
      241 ttctttaaag tttgtgaact catgttctgg gctactgcag cattttttag ttatgtagcc
      301 tgtgtatcag taggtatccc cttaactttt tgtgatcctc tcttgggtat cgcagtctcc
      361 ttatctacct ttttgatggc aattagcaca gcaagcaaat ttttagactg tttcgatgag
      421 ttccaatcat tagataagat taacgagcat gatcaaaatc aaaaggatac cgtacggttt
      481 ttcagtaaaa cgtcaaactc cacttcatca gtacgcaata aagaagctga agaagaaagc
      541 tctcagagat tatatccgaa tttacaaacc gtctaaatta ataaacaata ccgcatcaga
      601 ccaaagatta ttttggtctg atgttttggt ttgctttaaa gagcataatc aaaattaagg
      661 caatactgag tcaactcata tttaaatgtt ctctaaacag ccgttccgat aggaggctac
      721 tctgttacaa attaatctct gaaatctctc tattcatcct taatcaccaa ataaatcata
      781 acggctaaaa tagtaatgaa gatatgaaaa gctgtggtaa gggctgagtt gagcgtggga
      841 gacatccaca taccaaacca ctctcctcct atggacataa aaatcacttg ccaggttaca
      901 aaacccaacg taagacctcc aatagcccag ttcttggaat gattaaaaac agaagcactt
      961 tcatttcttg ccttaaacaa tttccacgca ccaattccac aaaatatggc ggttaataat
     1021 tccaggataa caatgataat aaatgcaaca taatgcagaa ctggactagt aattgctcga
     1081 taggtgatgg tggaatcagg aaatatatca ctcatggtta atacttttac cacagcagga
     1141 aaattggcgg aataatcagt gatgtttcca aatgcagtaa gtaagcaaaa aaaggaaaca
     1201 gcaacaacaa gcaacacttt tgagaatctg ataatcatca tttcatcctt tgaaacaagt
     1261 taaaaaatat gtatcaccta attgataaga acgttcgtgg agaagatgct tatgttcgtt
     1321 tctcaccaag ccaggttcgc tttcttcaat cttcgctaac caaaccgatg aatcaaaaaa
     1381 tcttagccca aacccttgcc cactttacta tcaattaccc cttctctttg agtatttctg
     1441 gcaaaacgta acaataaatg attcgctctg atcgtcgcct cgttcagagc ttcctccgga
     1501 ctcatcaaat ctgcaaacat tgcctctaaa acttcatcat taattccacg aatttgattt
     1561 tgcgggccca tgtgtttata aggtttctca ggtaaattgt cctcgagatc tgttctggct
     1621 aataataaag cgggatgttg acttgattgc acaatgctag cgtaaattcc ttttaacccc
     1681 aacggcaaat aacccgtatg ctcatgccag cgcttttgca cttcaggttt tgcaataaat
     1741 gcaaaaaatc tggcaattcc tcgatattgc tcttctgttt gtcctcctac agcccataga
     1801 gcagcacccc ctgccacatt cgcatatctt atcggactgg cacccgtatc caaaggcatt
     1861 gttgcaacac ccaaatgaaa aggaacaagg gcagacaaac tattataagc ccccgatgat
     1921 tgactaaata aaggacaaac actactggta aataaaatgg tagcatcatc aacacgacca
     1981 ccataacgaa aataatgcag atcatgccag cgctttagtc gttgaaaatg agccattaat
     2041 tggggagtat ggaaagcagc acgagcagga ttaccttgag ttatgggcaa cccatggata
     2101 gcaagaaaag attcaaataa cacccatcca ggataagccg tagtataagt acagtcataa
     2161 ccggcttttt taattttttc tgccattatt tccattgctg accaggtttg agggaagtta
     2221 tgcttgccat atcccacctt ggctaaaatg tcagcattat agtacagaac tggtgccgag
     2281 agattaaaag gcatcgccat taattgccca tccctgctat aaaactcacg cactgattga
     2341 ataaaatcat ctttaggcaa actgatcccc tgctcactca tcaacaaatc aacaggtttg
     2401 atcacaccag ggggagctaa cataattgaa gtgcctactt caaaaatttg aactattgat
     2461 ggtgcttgac gagctctgaa tgcagcagca aaattggtaa gagtttccgt ataatttcct
     2521 ttgtaaatcg gtttgactcg atattgattc tgacttttat taaaatcatc agcaagcaaa
     2581 cggacttcat cacccaaatg ccctgccatc gcatgccaaa aaaccagctc aactggtttg
     2641 gcctgcgcag gaatgacact cagaaaaact aacaaaagca aaaagagtat tttcatttta
     2701 acaagtctgg ataatcacta aagagagcat caacacccca atcaaataac tttttggcca
     2761 aacgtttacg gtttacagta taggcgcata ctacatagcc ttgatcctta actgctttca
     2821 ctcgatcagc ggttaatatc tttctattga aatgtattga ataacattcc aattgtttcg
     2881 ccttctgtaa ccaatcttta tcccactcat gcagcaagag ccctaaaggc atttctggag
     2941 caatactgcg gcataatacc aaagcatccc attcaaaaca ggaaacgaga ggtaattctt
     3001 tattttgggg ccaatgccga tggatatgac ttagcactga tacagtagtt tgctcaaccg
     3061 ttccaggata aggttttatt tctatgttcg cctggacatc ggaaaaagat aaccacttta
     3121 atacgtcagt aaagtgtgga atcttttctc ccttatactg cttcgaatac caagagccag
     3181 catcaagacc acggagataa tctgcctcca ccagtcccac ctctcctttt ccattcgtgg
     3241 ttcgtttcaa attgtcatcg tgaatgataa aaggttcacc atcaaggctg cacatcacat
     3301 caaactcaat gaaacgacaa cctaaagaca atgctttatt aaaagacgcc atcgtatttt
     3361 ctggagaata acccgatgct ccccggtgac cgataatttt ttcaaggatc aactaattaa
     3421 tctccctgtt tgcgattgga tgctgcttct accatagcgg tcaatattaa ttcattactc
     3481 actgtcaatg gtgccggagc agcagccatc gctaaagcat tcatttcttt cgcttgatag
     3541 gctcttggtt gctggggaat gttgtcaccc tcgaaaaaaa ccaggttact cacagagtaa
     3601 ttttgcttgg gataggcttt attcatacga tcaatttcat cattcacttg ctgataaagc
     3661 tgttcacgaa tcttggcacg tacgacttga gtctcttcca aactgggttt gaactccaca
     3721 ccactgatct cgtacttggc tccaggctta ctgaccgatt tggcattctg ataaatatta
     3781 gtcagagcag attgatcgac tcttgcctga gcttgtacat aaagtttctc aaggccagaa
     3841 ctatcctgag aacggtcaaa ctgcaccaaa tgccactccc ctttagcaat tttgtttaag
     3901 ctatccatta tgtcagcacg tgctttcacc aaatcagcat tacttagagt ggcattgata
     3961 ctgacattga gtaacgctgt ttgggtagta acccattttt tagcagaaac ttgaaaaaaa
     4021 atcttatcaa gcaccaaatc tggtgtctca ttatcagccc aaacaaaagg agttaagacc
     4081 attaaaccca gcatccaggc aatttttctc ataatatgct ccattcataa tgtttctatt
     4141 ggaattagtg tataccagca agctagaaga caccacagca gtttgaacat aatcactcta
     4201 aaacaaatcc tatacgacat tgcccgcaaa cagtcggatt tgagtacagg ttgagaaaat
     4261 aaacagagta aaaaagcgca gtgacattaa agtacatgag cattttttag aatacttttt
     4321 ctcaaactac accaaatacg gcattaccag caaacggtcg gatttgagtg caggttgaga
     4381 aaataaacag agtaaaaaag cgcagtgtac attaaagtac atgagcattt tttagaatac
     4441 tttttctcaa actacaccaa atacggcatt accagcaaac ggtcagattt gagtgcaggt
     4501 tgagaaaata aacattctaa aaaaagcgca gtgtacatta aagtacatga gcatttttta
     4561 gaatgttttt ttctcaagat gcgccaaata cggccgtttg ctaagaatta cattgattta
     4621 attgacgaat agatctataa cttctcccaa aattattgca agaggagctt aaaaatgatg
     4681 tctgttgaca atattaaaaa tgataaccag gttttggctg aagaagattt cctcgaatac
     4741 gcattgtctc atggatttgg tgatttacat tttaaggtgg atccgcaaac ggggatgaag
     4801 gctatgattg caattcatag caccaagttg ggacctgctt tgggtggttg tcgatttatt
     4861 gaatatccta atactgctgc tgctttgaag gatgctatgc gccttgccag aggcatgagc
     4921 tttaaggctg cttcagtcaa tttacctttg ggcggtggaa aatcagttat tattaagcct
     4981 aagggttttt ttaatcgagc agaatacatg catcggtttg gtgaattcgt taatgaattg
     5041 aatggcaggt acatcactgc tttggatagc ggtactgaat taagtgacat ggatattatt
     5101 gctgagcata ctgactatgt tgccagttta tccagataca atggagatcc ttcaccttct
     5161 acttccaagg gtgtgttaag agggattcag gctgctgttg cctttaaatt aggtaaagac
     5221 tctctcaaag gtttgcatat tgctattcag ggtcttggcc acgttggtta tgctctggca
     5281 aaacaattac acgaattggg cgcgacatta actgttgctg atatttcacc tgctgctgtg
     5341 aacatggcgg ttcaagagtt gggagctaaa gcggtcggca ctgacattat ccataaagta
     5401 ccgtgtgatg tatttgctcc ttgtgcttta ggtgcgatta tcaatgatct tactattccc
     5461 cagttacaaa ccagtattat tgctggcgct gccaataatc agctcgcaca cacataccat
     5521 ggcaagattt tacacgaaaa aggcattctg tttgctgtgg attatgtgat taatgcaggt
     5581 ggtttgatat tcgctgcctc taaatactta caaacgcctg aagacaaggt aagtgagcaa
     5641 attgatagta tctatacctc tctgactgaa atttttactc gctctgctaa agataattta
     5701 cctactagcg agattgctga tactttggct aaggaaaaat tggcttaatt tggataccgt
     5761 ttatgaaaca tttatccctt actcctgagt tgtacaaata tctgcttgat atttccttgc
     5821 gtgaacatcc tgctcttgcg gcattacgca aagaaacatc cactatggaa ctcgccaata
     5881 tgcaggtcgc tcctgagcaa gcccaattta tgcagatgtt gatacgtttg attcgtgcaa
     5941 agaaggtatt ggaacttggg acattcacag gttatagtgc cttggccatg tcccttgctt
     6001 tgcctgatga tgggcaagtt atcacctgtg atattaatga gggatggaca aaacatgctc
     6061 atccttattg gcgtgaagcc aaacaggaac ataaaattaa attacgcctt ggacctgctc
     6121 ttgacacttt acactcatta cttaatgaag gtggagagca tcaatttgat ttcattttta
     6181 ttgatgctga caaaaccaat tatctgaatt actatgaact ggctttaaaa ttggttacac
     6241 ctaaaggatt aattgccata gataatattt tttgggatgg aaaagtaatt gatccgagtg
     6301 atacgagtgg acaaaccaga gaaattaaaa aattaaatca agtgattaaa aacgactcaa
     6361 gggtctttgt cagtttgtta gctatagctg atgggatgtt tctggtgcaa cccatttaat
     6421 ctttaaaatc cccggcaagc aatacactgc attgctatgt aaataagtac aaggagaatc
     6481 tgataatgca aaataataac ccctgcggat tagatggctt tgccttttta gagttttcag
     6541 gccctgatag gaataaatta catcagcaat tttctgagat ggggtttcag gccgttgccc
     6601 accataaaaa tcaagacatt actcttttca aacaagggga aatacaattt atagtgaatg
     6661 cggcctccca ttgtcaggca gaagcgcatg cttcaactca tggtccaggc gcttgtgcaa
     6721 tgggctttaa agtaaaagat gccaaagccg cttttcaaca cgctatcgcg catggcggta
     6781 tagcatttca ggatgcgcct catgccaatc acggcttgcc agccatccag gctattggtg
     6841 gtagtgttat ttattttgtc gatgaagaac accaaccctt ctctcatgaa tggaatatta
     6901 cctcgccaga acccgtagtt ggaaatggtc tgaccgcaat cgaccatctc acccataacg
     6961 tttatcgcgg taatatggat aaatgggcca gtttctatgc ttccattttt aacttccagg
     7021 aaattcgttt tttcaatatc aaaggaaaaa tgactggttt ggtcagtcga gcattaggta
     7081 gcccttgtgg caaaatcaaa attcctttaa acgaatccaa agatgattta tcacaaattg
     7141 aagagtttct tcatgaatat catggcgagg gcattcaaca catcgctctc aataccaatg
     7201 atatttataa aacagtcaac ggcttaagaa aacaaggggt caaattcctg gatgtgccgg
     7261 atacttacta tgagatgatt aatgaccgtc tcccatggca ccaggagcca ctgaatcaac
     7321 tccatgccga gaaaatttta attgacggag aagcagatcc caaagacggc ttattactgc
     7381 aaatattcac tgaaaacatc tttggaccag tcttttttga aattattcaa cgcaaaggca
     7441 atcaaggatt tggtgaaggg aatttccagg ctttattcga agccattgaa cgagatcaag
     7501 ttcgacgtgg tactttaaaa gaattgacct agacaaaccg ttaaaatgta atgatgctgt
     7561 ttttcaggca taagccagga gatctatatg aaactcgcca gtttgaaatc aactcgttcc
     7621 cgggatggag agctttgcat tgtcaacaga ttgctgaccg ctgcaatacg cgttcctcat
     7681 atcgcatcaa cacttcaata tgccctggat cattgggatg ttgttgaacc caaattacaa
     7741 ctggtttatc aacagttaaa tgataatcaa atcgaagacg cctttgcgtt tgctcccgag
     7801 ctatgtgcct cgccattacc ccgtgcttat caatgggccg atggcagtgc ctatgtaaac
     7861 catgtcgaat tggttcgtaa agccagagga gcagaaatgc ctgctgattt ttggaccaat
     7921 ccactcatgt atcaaggcgg atctgatgct tttctggggc ctcgtgatcc tattttggta
     7981 agctcagaag cctatggaat tgactttgaa tccgaggtgg ctgttatcac tgatgatgtt
     8041 cccatgggga ttgatcatac aaacgctgcc catcatatca aactgttgat gttggttaat
     8101 gatgtctctc tacgcaatct tatacccgaa gaactggcaa aagggtttgg gttcttccaa
     8161 tcaaaacctg ccagcagctt ctccccggta gcgataaccc cggatgagct aggttcttac
     8221 tgggatggac agcgtgtgaa cttgccatta attagtcatt ttaatggaca attatttggc
     8281 caacccaatg ctggaattga tatgaccttt tcttttccag aactgataca gcatgctgca
     8341 aaaacaaggt ttttaactgc gggcactatt ataggttctg gaacagtatc caaccttgac
     8401 cgcagcaaag gttcttcttg tattgctgaa aagcgaatgc tggaaatgat agaattcgga
     8461 caagcaaaaa caccgtttat gcgatttggg gaccgaattc acatagaaat gctcgatggt
     8521 caagggtatt ctctatttgg ctccattgac caaatagtga cgaaatacga gaaggattcc
     8581 ttatgatatt gtatgactat ttccgctcta ccgcatgcta tagagtaaga atcgctctta
     8641 atttaaaaaa aattgtttac gaaaaaattg aagtgcatct ggttaataat ggaggcgaac
     8701 agcatagcct tcagtatcat caaattaacc ctcaagaact cgttcccagc cttgacatca
     8761 atggacaaat cctgagccaa tcaatggcaa tcattgatta tttggaagaa atctacccgg
     8821 aagtaccatt gcttcctaaa gatcccttta tgaaagccaa tctaaaatct ctggctttca
     8881 ttgttgcttg tgatatgcac cccctcaata atttaagagt gctgaatcgc ttaaaagagc
     8941 aatttaaagc cagtcaagaa caggtaatgg aatggtacca tcattggcta aaaacaggat
     9001 ttgatgcttt tgaggaaaaa ctcagggcta tgaagcgcga caagccagtc tgctttggaa
     9061 atgaggttag tctggcagat gtttgtttga tcccccaggt ttacaatgcc caacgatttc
     9121 attttgatat ggcgagttac ccaataatca atcaaatcaa tgagtattgt ctaaccctgc
     9181 cggcattcca tgatgcggca ccagaagcca tttcatctta aaaccatgct cttctcaact
     9241 ctaccgtgat tacgccttta ggagttttct ccaacatact ccaggctgtt ttacatcgtt
     9301 gcaggtaacc aattaaaaca ttttgattgc tgcaacggta ccttacaggc attgaatcat
     9361 attgctcgtt agaatttaca gccaaacagt actcaccatt aaccaattca atagcccagg
     9421 gaaaagcgcg agacatatct aaggtcttat ggtgttcatt attcaaaggt atccctacgt
     9481 taatttgtac gctatcacca acccatgggg attgggggca tacagcttcc atttttttac
     9541 tacctgcttt cacaaaacag ggatcaaata attggtctcc agcctgacaa cgccatgcgt
     9601 cttcacgtat aatgatgtgg gattgttcat aacattggcc agacattatt tttttaacga
     9661 caggtggtgc ttgttcaaaa acatcaccaa aaggcctgta taattttaga acggtatcgc
     9721 tggcgctggc taaaaaagaa aaaaaacata gcaatacgag taaaactctt tgcagcatgg
     9781 tgttccccca cggattttat ttagtgtatc atcacatttt aattcagggt ttaggaaata
     9841 ctacaaacgg gagaaaatca atccctcgaa ggattggaat atttcctaag ccctgaatca
     9901 atcgttttcg attttggagt atataatgac agaagtcttt actataaaac aatgtctcga
     9961 cggcgaaatc agtattgatg aaaccgttac ggtaagagga tgggtgaaaa cccgaagaga
    10021 ttcaaaagct ggcttgtctt tcatcagcct tcatgatggc tcctgctttt ctcccataca
    10081 aatcgtcgct accgatcagt tatccaacta ccacaaagag gttacaaaat taactgctgg
    10141 ctgttcaatg atagcgactg gaaaattggt ggcatcgcaa ggaaaagggc aattttttga
    10201 aatccaggcc gaatccattg aagtggtggg atgggttgaa aatccagata cgtaccctat
    10261 ccaagccaag cgccatactc ttgaattttt aagagaagtg gctcacttgc gccccagaac
    10321 aaataccata agcgctgtca ccagagtgcg ccattcattg gcacaagcca ttcaccgttt
    10381 ttatcatgaa caaggctttt tttgggttca tacacctatc attactgcca gcgactgtga
    10441 aggggcagga gaaatgttca gggtatctac tctggattta ttaaatatac ctaaaaatga
    10501 taaaggacaa attgatttca gtaaggattt ttttggcaga gaaacattct taactgtttc
    10561 tggccaatta aatgtggaag cctattgtat ggccatgtcc aaagtataca catttggacc
    10621 aacatttcga gctgaaaact ccaatacaag ccgacatttg gcagaattct ggatgataga
    10681 accagaaatc gcttttgcaa atctcgaaga catttgtaaa ctcagccaaa atatgctgcg
    10741 ttatctctgt aaaactgttc ttgaagagcg ttcagatgat atggactttt tcaaccagtt
    10801 tgttgctcca ggatgcatag agcgcatgga acatattgcg gattctgaat ttgaaattat
    10861 gacctatacc gatgcaatca aagcacttga ggcgagtgag caaaagtttg aatttccagt
    10921 gagctggggg ctggatctgc aatcagagca tgaacgttac ctggctgaag tactttgcaa
    10981 aaagcctgtc attgtgacaa attacccgca agaaattaaa ggcttttaca tgcgccttaa
    11041 tgacgatgga aaaacggtag ccgctatgga tgttttagct ccaggcattg gagagattat
    11101 cggcggcagc caacgcgaag agcgattgga aatactggat agacgcatgg acgagtgcaa
    11161 cctgaataaa gaacattacc aatggtatag agacttgcgt cgttatggca ctgtgcctca
    11221 tgccggattt ggcttgggct ttgagcgctt gatcagctat gttacagggg tatcgaatgt
    11281 gcgagatgta ataccattcc ctcgcacacc aggccatgcg gattattaaa tcgataaggc
    11341 gcttaaagaa tcttcctcaa cttcagctgt ttgttgttga ggttgattcc cctcagacag
    11401 caatcgccgt ctatttcttg gcgaaaatat tttttctaat atctcatcat ctgcattact
    11461 cgcaagcggt cctcctgcat ccactgtatc ataatgttcc ctattgaaat ctggtccagg
    11521 tagaaatcct ttgttacttg tctgagttga agaagatatt tttgaatttt cggttgagta
    11581 aggataaaaa atagaaaaac ttcttctatg tgactttaca ggtagattac gctcggattt
    11641 tctcaatggt tgggcttttt tctcatcctc aacagtgctt gtaatttcat tgatctgaat
    11701 tgttttatct tttttctcat ccattgttaa cactccttat cttattttta ttctctcgca
    11761 tacaaactgc cttcatgcaa aacaagtact ctatccatac gttctgcaat ccgttgatca
    11821 tgagttacta taaccaaggc cgtattcatt tttttattaa gttccaacat caaatcaaaa
    11881 actttactgg ccgttgcctc atccaaattt ccagtcggct catcagccag aacacaatgt
    11941 ggctgatgca ccaaggctct cgctatggca acccgttgcc tttctcctcc agacaactga
    12001 gcaggcttat gcgttaatcg tggtttaagc cctacctgtt ccagcatatt aatggctttt
    12061 tcttccgcat ctttcacagc catacccgcc agcaataacg gcatcatcac attttcaagt
    12121 gccgtaaact caggtaacaa atggtggaat tgataaacaa atcctaattg ttgattgcgc
    12181 aattgacaac gttgcttttc atttattttt tgccaattga cattgccaag agtaatcagg
    12241 ccgctggttg gtttatccag cccccccaac aaatgcaaaa gagtactttt tccagaacct
    12301 gatggcccga taatagcgat cctgtcccct tttgttatgg ccaagtccac tccttttaac
    12361 acctcgactg ttgaagtgcc atcatggtag gacttataca gtttttgact ggttaaaatg
    12421 gtgtcattca taatgcaaag cctccgcgat gactgtcttt gatgctcgcc aggcaggata
    12481 aatggttgca gcaaaactca ttgataatgc catagcacaa acttgccaca gatcacgaaa
    12541 cataatttta gaaggcaagt aatcaacaaa atagatgctt gaagacaaca ccttcacctg
    12601 gaaaaaggac tgcaatgcat taactatctc cgttgcatta ttggccaaca ctaagcctcc
    12661 taacagcccg agaattgtac caaccagacc aaccatcatt ccctgtacaa taaaaaccca
    12721 taaaatggta gaaggagtag cgccaatagt tcttaaaatg gcaatttcag cttgtttgtc
    12781 attgactacc attaccagag aggacaccaa attaaaagct gctacagcaa taataagcaa
    12841 taaaatcata aacatcatcg ttttttccat tttaactgct tcaaaaaaag ctccaaattg
    12901 ctgggtccaa ttgcctactt gatatccttc tcccaaaaga tcggataatt cataagacaa
    12961 ttcaggtgct ttgtatacat tattaatttt catttttata cctgaaacgt cattcttatc
    13021 catttgcatt aatttttgag catcttcgat attaataaat gccaatttag tatcgaaatt
    13081 aaatcctgta ccagcagaaa aaacacctac aacggtaaag cgcttgaatc gagggatcat
    13141 accggcagga gttactgttg cttgcggaat cattatcgta accttatccc caatcatcac
    13201 cccaaggctg tccgcaagac cttttccaag aataatccca aaatgtttca gattatccat
    13261 attacctgcc agtagtttct tattgagatg agttacagat tgttcttttt caggcagaat
    13321 accggtcagg acgataggca aaacctggcc ttcatgagtt aataatcctt gacttccaac
    13381 ataaggcgca attgccttta ttccaggaat tgtttcaacc tttttaacca cttcaggcca
    13441 atcacttaat cgctcatcag gacctgttat ggtgatttca ggcgccatcc caaaaaaacg
    13501 acgatgaatt tcctgatcaa aaccattcat cactgataaa acagtgatta aaaccatcac
    13561 tcccatgcct atacccaaca tggaacttag ggaaataaag gaaacaaagt gatttttctt
    13621 acgtgaacgc gtataacgta atccgataaa aagagccaat ggtttataca taatcgtatt
    13681 cagctggatt aaagttagta ttgtacagta cttatagaaa aggccaagat tgaagcctaa
    13741 agattttaaa gaacttatca ataattcatc atcagacaac ccacatagtt atccacagaa
    13801 tctgtggata tctaataagt cccattcatc gattagagta tcaatcaagc aaataccaac
    13861 ttacataaaa tgaatatccg ttacgatata gcagatgcta cacataatca gaaaattatt
    13921 aaatggaacg ggtatttgtc agtgaaattt taaaattaat tagtaaatgt attatttaaa
    13981 tggagaaagt aagttgttag ggtgcttcgt tttacgaaga acaatgaaat aactaaagaa
    14041 gtttcattgg aagatgaaat taattcaacg tagggttatc attgcccttt aaaatgggga
    14101 ttagtcaaat ttggtgccca gggccggaat cgaaccggca tggtgttacc accgagggat
    14161 tttaagtccc ttgcgtctac ctgtttcgcc acctgggcat tcactgtttt tttaaatgga
    14221 ggctgaggcc ggaatcgaac cggcgtacac ggctttgcag gccgctgcat gaccactctg
    14281 ccacacagcc tcaaatctat atactttgct attgctctaa aaaaaaagcg acttcgatgt
    14341 cgcttaaatt tggagcggga aacgagactc gaactcgcga ccctaacctt ggcaaggtta
    14401 tgctctacca actgagctat tcccgcgtct ctacgggatg agattctacc gaaaataaat
    14461 accctgtcaa cagcaaaatt aacttttttt cattaactct ggccaggcaa tagccaaata
    14521 tatcaccatg gaccaaatcg tcaaaatagc agacacataa agaagaataa accctaagct
    14581 cccccaccag gaaacaaggg gattaaatgc cagcaataat accaaagcga tcatttgcaa
    14641 agaagtttta actttcccta tatatccaac ggcaacacta gcgcgacttc caacttcagc
    14701 catccactcg cgcaaggctg aaatcacaat ttctcgtcca acgattacga ttgcaggtat
    14761 agtgatataa tctatatcct tcgctccaac taacaataac aagcttgtcg ataccaataa
    14821 tttatcggcg acgggatcaa gaaaagctcc aaaatgagac atcatttcaa gtttgcgcgc
    14881 caaataaccg tctagccaat cagtaatgct tgcaatagca aaaagaacag cagcggtagg
    14941 atgagcccat ttgaaaggaa aataaaatac cataataaat actggaatga tcgaaatccg
    15001 cattaaggtt aatatattag gcaaactggt taacgtactc actgctgaac atcccccttt
    15061 ttttatgctt ccggcaacat cagataagct gacaatagct tataatcatc aaatacagcc
    15121 aaagcacctg ctgccttaag cgcatcctct tgctgatggt aaaaatccat gcctatagca
    15181 ttaacattga ttctttttgc catttccata tccgttgtag aatcacctat catcaaagtt
    15241 gatgaaggat ctctcccgaa ttcctccatg atttcctcaa gcatttgcgg acaaggtttt
    15301 gccggtactt gaccagcaga gcgtgtcact ttaaacaatt gatcaagctc agttgcctga
    15361 agagcacgga ttagagaatt gtggccttta tttgtagcga tggccaaatc gacctttgct
    15421 tgatgaagtt gttggataaa ttctaatact ccaggtatta aacgcacttc tgttggccgt
    15481 gaaatcattg cggattgcac agcctgtaac agatcttcct gctgcccggc agttagattg
    15541 ggtaatgtct tttttactgc ttgaaccaac cccaagtcta catattttct tgcttgatat
    15601 gggtcaatat caccaaagcc caacattttg gcttcagtag caacagtatg taaaataact
    15661 cccaatgtat cagcaatcgt gccttcccaa tcaaaaatta ctaattgata tgcactactc
    15721 atagctcaat aggctccttg cacgcagttg ctttaaagta tctccaaatc catcgtctac
    15781 atcagcttga aatacatgca ttaccccatt cagattgaat tgaatagacc tggcatgcag
    15841 ataaagacga ttctgttgat aatctatacc ttccacctca cccaccttag agccgtattt
    15901 ctcatcaccc acaatgacat gaccaaagta agcgctatga acgcgaattt gatgtgtccg
    15961 tcctgtttta ggagaggctt ctacccaaca ggcctgatga taattttcca gcaatttaaa
    16021 atctgtttct gatgccttac catctttctg aacggaaaca accctttctc cggatttcaa
    16081 tgtgttcttc tcgagaggag ctgtaattgt gattttcttt ttaccgtccc aacgatgcgt
    16141 taataaagcc caataaattt tctgcacttc gcgggcttcc aatagcgctt gaatggctcg
    16201 taacgtgctt ctttttttgg ctaataaaag acaaccggaa gtctctctat ccaatcgatg
    16261 taccaattcc aaataggata aatcgtgcct tgtctttctt aatgcttcaa taaccccaag
    16321 gcttaaacca ctaccaccat gtactgcaat acctgctggt ttattaatta ccaacaagga
    16381 agaatcttca aaaatgatac tatccttcaa gcgttttgcc aaattatcac ttacaaaaat
    16441 ttctttagaa tcagtcattc gaattggcgg tattcttata ctgtccccaa tttgtaaacg
    16501 actgttagct tgtgctcttt ttttattaat ccgaacctcg ccgccacgaa taatcctgta
    16561 gatatggctt ttaggaactc cttttaaaat gcgaatcaga taattatcca aacgctggcc
    16621 atcctcttcg gagccaattt ctttataact tacttcactc attgtcaata aacctgtttt
    16681 ctgagcgtga tttttttgat atgattagat atcgtacggg aaaatccctt acgaccgaat
    16741 tataacgtat aaatagaaaa atagtttaat aatcgattca taatcgataa aagatttgta
    16801 attaattagt taaaatactt gcgttcatct taattttcca tcattaatga agaatgaaaa
    16861 attaacagaa tagcataaat ttagttaatt tgatagcaaa tcgaactaaa gaagcagctg
    16921 ctcaagcgaa tataaaacag gttgaattca ataagaactt gaaccacaag ttaaagaatc
    16981 acctctgttt ttttcatcgt aatacgcctg agtagatgct tggaagaaaa aacaaaatgc
    17041 gcttccattt aagtcgaaga tataaggaag cgacccaata ccagagtaaa attgataatt
    17101 atttcgacag atacctacgc atgccagaca tgctttatac caattacgaa ttattgccgg
    17161 gcttttgcac gcctgatgaa aggccttttt tttccaaccc acttaatctg taaacggtac
    17221 atcatacagt gtgcaccttt aactactgag gtaacatgcg tacggtctgt ccacaaggtc
    17281 atgggtcagc ttaaagttcc tcaccctgcc agcttaataa cattgttttt caaaaacaaa
    17341 attgagcaat tagggaatag gctgttatca tttcaccaga attatagaga ccaggatatt
    17401 aattttctga ccccctctct cggcagattc aaaacgaatg caagatatgg gacccggaaa
    17461 attaacttca tggcaagaaa actgtagaaa tgatcataca gctatactga gccttacact
    17521 atctcgatgt aaatgcatgc gccctaaata tggggttata catggaaaaa atgctaatca
    17581 atgcaatgca aacagaagaa attcgtgttg cactaattaa aaacaatcaa ttatttgatc
    17641 tggacataga atgcccggga gaaataaaga aaaaaggtaa tatctataaa gccattgtaa
    17701 caagaagaga gcccagtctt gatgctgttt tcgttgaata tggttccaaa cgccaaggtt
    17761 tcttgcccct aaaagaaata gcgcctgaat atttaagcaa aaatccggaa gagtttggag
    17821 atgagaaacc tccaattacc agcctcatcc gtgaaggtca agaattactt attcaagtgg
    17881 ataaagaaga aagagggaat aaaggagcag cactgacaac ttttattacc ctggcaggtt
    17941 gctacctggt tttaatgccc aataaccctc attcgggcgg aatatcaaga cgtattgaag
    18001 gtgatgagcg tgatgaatta cgcgaaacat taaatgcttt aacactccct gaagacatgg
    18061 gtcttattat tcgcactgca ggtgttggaa aaagtctgga agaactgcaa gatgacttaa
    18121 acatgctttg caatcagtgg caatccatca aggaagctta taattcagag ctggcacctt
    18181 gcctgattca tcaagagggc gatgtcatta ttcgttccat tcgtgacaat ttgcgaaaat
    18241 ccatcagtga aattattatt gatgatcaaa tatcctatat caaagcaaaa caatacattg
    18301 aacgcgtaaa accagaattt ttacctaatc taaaactcta taacagcagc atccctcttt
    18361 ttaatttcta tcaaattgaa agccaaattg agacagctta tcagcgccag gtcatgcttc
    18421 cttctggagg tgccttggtc atagatagaa ctgaagctct ggtatcaatt gatattaact
    18481 cagccaaggc tacaggcggt gccgacattg aagcgactgc attaaatacc aaccttgaag
    18541 cagctgatga aattgctcgc cagcttcgcc tgcgtgattt aggtggtttg gtagttattg
    18601 attttattga catgagttct agtaaaaatc aaagagaggt agaaaatcga ctcaaagaag
    18661 cactgcaggc tgatagggcc agaattcaag tagggcgaat ttcacgcttt ggccttcttg
    18721 aaatgtcaag gcaacgcctc cgattatcat taggagaaac tgcccaggaa atttgtccac
    18781 gttgtgaagg tagaggaact gtacgcagta tacagtctca tggcttatca attacccgcc
    18841 ttatcgaaga agaagcgctc aaggaaaaaa cagctgagat tcaagtacaa ttgccaccag
    18901 aaatggctac attcattatg aatgaaaaac gtaattttat cagggacatt gaaaaaagac
    18961 atggtgtcag tgttgttgtt attgcgaatc cttatatgca ccctccacaa tatacgatta
    19021 ctcgcctgaa agaagacaac gtaggtaaat ctaaaaaacc aagttactca ttgattcagc
    19081 aacctgaagt acatgttccc agcagtgata aagaacccgt gtcacatgat gaacctgctg
    19141 tgaaatcatt tgccgaacac agggtcatca aacaccctca aaccagtttt attaaacgat
    19201 tatggaccag tttatttggt ggtcattcag ttgcccacac cgtaactcaa gaacctggta
    19261 aaaaacatca tcgtgctcaa aaccaaggta aacacccatc gtccaaaagc tcaggaggag
    19321 acaaacgtca acaaggcagc aacgcaaatc gtagacgtcg ttcaggagga ggccagcagc
    19381 gttcttcatc aggaaataca aatgctgcgg ccagcacaca tcaacaacga aaaaaatcta
    19441 acgcgaatca gcaaagtcat ggcagccaac aaagcaatcg ttcaattcct gcaaaaaatg
    19501 aaggcaacaa aaaaagggaa gccatactgg ctaaagagca agaggagaaa taggccgcca
    19561 tattaattat ttgcctattc accttttcaa taagcattta cccgcacaat ccagttacct
    19621 ctggattgtg cgggtaaaac acagcactct ttcttgtcag gataccgatt ttaaaattgt
    19681 aactatttgc gggatatgca aattcttctg tttataagcc tgatcatttg agtcgtcttt
    19741 cccgcgaagg cgggaatcta tccataaagc tagtatatag gttaattttt agatggattc
    19801 ccgccttcgc cggaaagaca gaggacttaa tattcttttg attgcatatc ccttaaccgg
    19861 ttacttaaaa ttataacctt taaaacagac tgattcttct tccttaaatc cttctcttaa
    19921 ttgactaaag cgatagtctt attatcctct actacctcta ctttaggctg ggtgacttta
    19981 cccttgggcg tgctatagat tccatgagaa agcaaacctg agtggacagg gtcattactg
    20041 ggtttcacat tctctttgtc tactattctt tccaatttat gcaagcccaa tttttccaaa
    20101 cgttcaacct gttgttcctg acgctttaac agttcgctat aacgatcatt gctttgcctt
    20161 aattcttctt tgactttttt aagttcttct tctgccttgc aaattcgttc agctacttga
    20221 tcaaagctaa gctgtttgtc attcaggaat ccatccagtt tcttttgaaa agaaacacgt
    20281 tgctcttcat cagcaatgac cgttccagtt aaagtctgga ctgtaccttc caacgttttc
    20341 gcaactgttt tagctttttg aacttcgaga cccaacgaca gtctgacttc atgtaattca
    20401 gctatttttt cggctaactg caattggctt ttttcgtatt cctttctaat ttttgacaat
    20461 tccttttgat ttttttccaa taaatcagtt tgttttgttg tagattcttg aagttttttg
    20521 acggtttgct ccatttcatt tgtatcttta cggagcattt tctcaatttc catatataac
    20581 tcaacctgag cactcagtga ttccacttca ttgcccagcc tgtcaatgtt atcagttaat
    20641 ctgaggtttt cattcttaaa tttctcaatt tcttcagcaa attttttgcg aattgcatcc
    20701 aatgcctcaa tcgtaatttg taacagatct gccaaaccaa aaagcccttc ttttaatcgt
    20761 ttggcaatat tcacattgca tgtatggtgg tcgtccaaaa caattgcacc tgcggtatag
    20821 gttagtcctg taactccccc tattactaac aagacgccga tgtgcgcaaa taatcctact
    20881 gccaaggtag gcgctgtcag gacaaggcca cccgtgactt tctgccacat aggcagttcg
    20941 ccccaggctg acgcagcgcg agtaatcagg ctgggatttt ccgctatact gtctactatt
    21001 gattccaggc tttctttaac ttggctcagt tgtttttggg tatcgacgat ttgggcaaga
    21061 ctagcgcttt caatttcagg agtgccagta cttgtcttag gatccttatc agtactgtcc
    21121 aagctagaag tagtgattgt tgatttgcta tcatgttgga tcaatacttg aagctctgtt
    21181 tcattagtag ccatatttac tccttacgat taaaaat
//
LOCUS       DBNCWK010000044        20988 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.044, whole genome shotgun sequence.
ACCESSION   DBNCWK010000044 DBNCWK010000000
VERSION     DBNCWK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20988
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.044"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            27..1373
                     /gene="sdbB"
                     /locus_tag="AC06IO_001435"
     CDS             27..1373
                     /gene="sdbB"
                     /locus_tag="AC06IO_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbB"
                     /protein_id="HIO9874630.1"
                     /translation="MAKTITKDSFFHSRLQQFVANSLFPAGAGDWYKNKGYGKKGEVD
                     DEAPFRDFVEKQKTDKKSHYYKEFQGLDLSLKKVKTKLVSGESCQLEVMKCQPESHEP
                     KKPGTGKHIVYFPGANTYYQACFRDISTACKETGATVHAFNFPGTGLSSGKVREANDL
                     INAGISVVSSLIKQGVHPDDIILQGDCYGASIALEVKKQLEDQADIKVRAIMNNVFKS
                     FKAAVCDMITQSPWLPNILKSIVKRLLEFTGWHVTPGKKYKHADPYQCHIQHLGDQTL
                     ESSTLSGKVSKYHHEIQTGQTKSQKRAPITDTCPEEYREDRDELDRKHYVRVKESAKE
                     RLASKFGVDKFGRVNAHFADLCELEMLDGQSVYQGFANDYIARSNAYIEKHPQKGIKE
                     IQDDLRKLHYLQPADSIEITEDEAQDFNTVVDLITDEQQLRHDRVNDNTIGKSISM"
     gene            complement(1400..1957)
                     /locus_tag="AC06IO_001436"
     CDS             complement(1400..1957)
                     /locus_tag="AC06IO_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIO9874631.1"
                     /translation="MRILEKNIKKTPWYLKPFFWNQKRKYGQILEPALIWARIPRLFL
                     AVAAMYGVIDRKKSPLSPILRSLITVRVSQINWCAFCVDINSATLIKRAGSEAQYKQL
                     AKWRSSSLFSPEEKAALDYAEKVTYSDQRPDDECFNQLKLYYSEQEIIELTALIAFQN
                     MSSKFNSALGVEAQGFCSRQLTGKS"
     gene            2006..2590
                     /gene="rdgB"
                     /locus_tag="AC06IO_001437"
     CDS             2006..2590
                     /gene="rdgB"
                     /locus_tag="AC06IO_001437"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIO9874632.1"
                     /translation="MKEIILATSNPGKIKELEQLLAPTLCIPQADLGISDAEETGLSF
                     IENAILKARHASSLANKPALADDSGLVVPSLNGEPGIYSARYAGIKANDEDNIQQLLS
                     KMADLSQEQRQAYFFCAIALMQHAKDPTPLIATGVFHGVISVKPSGTNGFGYDPVFYL
                     NEYQCTAAELPAKIKNRISHRAKALNQLRALLPD"
     gene            2592..4325
                     /locus_tag="AC06IO_001438"
     CDS             2592..4325
                     /locus_tag="AC06IO_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIO9874633.1"
                     /translation="MNSEVDTLFAQAYKLQYEGHLPQAISLYEQILAQSPKHTETLHF
                     LGLTYAQLGDMENAILYFLQARKINPNDVGILNNLANAYKKAGQLDEAIKYYQEAIEI
                     KPEYVQAHNNLAATYALLNNYQKALHHYVIAVNTEPDFSAAHFNLGLLLLKNQQLSAA
                     KTQFNNVIALNPQHREAQFYLGILHLEDNLLAEAEQAFHKVLEQDHEHVQSLINLGVI
                     ALKREQNQLAVDYFTKALALDNEDIDARNNLAATFMHHDRFENALMHYDVLLKKEPNN
                     LEYLYNSGVAQMALGHLNEATLLFDQILTLESDHTPSLNNLAAIYLKMDMRETAREYL
                     ERALAINPDDVVSRHMLNAITGATSVNTTEKYAQNLFNNYALYYDQHMQGELHYKIPH
                     HIGRLIHQLQLLQTSHSLDLGCGTGLTGIVLREISKHLTGVDIAEKMIARAKEKNIYD
                     LLVCSELIDFLRKDKNDYDLAVAADVLPYFGNLDDIFNSINQHLKQEGYFIFTTEIST
                     TTPWKLESSARFSHQPEYIESLINKYQFHLIKQEKIPGRTQNKQVLEVMLYCVKKSAS
                     RFVANRTYENP"
     gene            complement(4568..5956)
                     /locus_tag="AC06IO_001439"
     CDS             complement(4568..5956)
                     /locus_tag="AC06IO_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIO9874634.1"
                     /translation="MTYQQKQIPRSVFRAYDIRGIIGKELDENSFYSIGLAIARYLQK
                     LNRRQIFVARDGRLTSYALATALKEGLLDSGIDILDLGAVATPVMYYATHTQGIDSGL
                     MVTGSHNPADYNGIKLVLAGKTLIQDEIDTLYDLLGEVKQSAVRGTETAFDVIEGYIQ
                     RIVSDIQLKRPLKVVVDCGNGVAGPIIPRAIAKLGCEVIPLYCEVDGRFPNHHPDPSI
                     EANLVDLKAAVANHQADIGLGFDGDADRLGLVTNKGEVIWPDRLMMFYSREILSRNPG
                     ATVVFDVKCSSHLEKEIQAAGGVARMCPTGHSIVKAVMKKEQAILAGEMSGHLFFKDR
                     WYGFDDALYSACRLLEIISSSHLTVSEQFELIPNSVNTPEIKIAITDEEKFDFIQRFS
                     EQADFPEGRILNIDGLRVEFPNGWGLLRASNTTPCLVARFEATDKDHLEQIQYLFKKQ
                     IQRLDSELDLPF"
     gene            complement(5957..6415)
                     /gene="dut"
                     /locus_tag="AC06IO_001440"
     CDS             complement(5957..6415)
                     /gene="dut"
                     /locus_tag="AC06IO_001440"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIO9874635.1"
                     /translation="MHQVIQLKILDSRIGDTIPLPAYATDGSAGLDLRVCISEPMQVA
                     PQQTVLLPTGIAIYIADPKLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKISCW
                     NRSQEHFTVNPGDRIAQLVFIPVVQASFEVVNEFTESSRGEGGFGSSGRY"
     gene            complement(6442..7638)
                     /gene="coaBC"
                     /locus_tag="AC06IO_001441"
     CDS             complement(6442..7638)
                     /gene="coaBC"
                     /locus_tag="AC06IO_001441"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIO9874636.1"
                     /translation="MQDFINKKILLGICGGIAAYKSAHLVRELTRLGADVRVVMTHSA
                     QQFINPLLMQALSGNEVRIDLFDTQAERAMGHIELARWADYLLIAPASANCLAKMAQG
                     LADDLLSTLYLVTETPVIVCPAMNRSMWAHPATKANCELLKERGVFFVGPEEGSQACG
                     EHGLGRVSEADQIVNTLRLLDVNQLLHGKRIVITAGPTRESIDPVRYLSNYSSGKMGF
                     AMAEAAAMAGAQVTLISGPCHLQTFSDIEVIRVESAQSMLEEVNRHVQQGDVFIGTAA
                     VSDYKVLNPASEKMKKNENSELTLQLVRNTDILSEVAKSGKASFVVGFAAETTDVISY
                     AKEKMKNKKLDMIIANQVGRGMGFESDVNQVTVITKNKQIELPLTHKTRLAGQIIAIL
                     AASLQN"
     gene            7715..8398
                     /gene="radC"
                     /locus_tag="AC06IO_001442"
     CDS             7715..8398
                     /gene="radC"
                     /locus_tag="AC06IO_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIO9874637.1"
                     /translation="MMVAHTAQQLDLREKLLTNGVHSLSDIELLAVFISSGNNKKSCL
                     QLAYELTKHLGNLRNILNADLQSFKSIHGLGEVRYAQLQAAKEICHRSDFIDLQKEIR
                     LSNTQQTYAFLKKRLRDYKNETFAALFLDNQHRIIAYEELFSGTINTATVYPRPIVER
                     VLQLNAAALILAHNHPSGLSDASQQDLAITKRIRDALDLVDARLLDHIVIGDNEVYSI
                     FAENKWVCN"
     gene            8750..12640
                     /gene="lepB"
                     /locus_tag="AC06IO_001443"
     CDS             8750..12640
                     /gene="lepB"
                     /locus_tag="AC06IO_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS GTPase-activating effector LepB"
                     /protein_id="HIO9874638.1"
                     /translation="MLTYQGKEIVRFKEKTGGKNKSDVDGFYKDSDGRKFFIKKPGDP
                     RELFTELFAGLLLKEFMKRGLIDESYFPSLICADVIQFEDKSYGLIQPLVSFDELHKI
                     IGTSYGDGSDRNTIKETFFGPGYYAGITKQNKYFGLSMALMFSLLLGAHSVHSGNIVV
                     LKGEGEGEEKSKQFGRIDWGDAFRYFAHRNNNDNLLYAYENRGWFNYKSLTKDYFLNY
                     KKINGLFPAMAEKARQLQSKLNPELLIEIVTSALKNIPADLIEEKTKIQLAAYMCMDS
                     FKEVTFGPKGNCKDFAIAMATLLENRIGKIAVLKDMSPLSNPEELYQSILELKPLTLL
                     MTSSTSFSETINQWADILKTTDMEKFSFDSNPINLPELVKQFNLYVDELAITCEANNV
                     WAKERIDSTPNLFALYDNSGGEAIHGHAFVPYYKESIVLRRLFTVDPNTFNLSRFAAF
                     EGPCQLYCKEHKDSAWVKIQTLLTLGNGIINTLKIIKQAQAFGIDEAVTENLKALKEQ
                     FIAFQLAEADVKESLKAPSFAEPLPDKESEFFYPIDEKALAKMNGYQLATICLEELNS
                     PKPSPLIERILSNKKFWKRINSAFESGVFKGRTDNPAGKIAKIHEWHQLLQISGKKTA
                     GQIDELQKIVISLQSKIKKQTIEFEELEATLIQIKEKYQLLEKMAEQSEHEKSSAQSI
                     IRSLNLELGQLKLQLQEQEKLQFQLKELKEKIHEQTTLSKRLGEELQTQKKTNTHQEE
                     TIQRITKEKSLADSSLESLRKELHELAKKERSLHKTLEEKQLQVQELEEQLAEKEKEN
                     LALKKADKQSQHEKSLDKSAIESLTSELNQLKLELQKQETLELQLKSLRKQIQEQTLL
                     VEGLKEELQKQKKSNTHQEETIERITKEKSLADSALESLRKEMHELTRKNEENQLKLT
                     KQVHSLSEQLEEKQLQIREFEKQLQEKEKRVEQSEKEKASEKRTVASLREQVSNLKLQ
                     LQQLGEVIQEKEKGSSLISQQSKQIIALQEIVEEQKRQLEELKIKIQELVSANQELGK
                     QNQSLSKENLHNKNTVEDLKKKLTELNVQLEQLHQSSSEKEQTIRKLREELIKKDSSL
                     KQNEEMQLAQKHLQEEIDRLQKEIKQQQLNTNQLESIIAQSKEAEKRYQQALQQKKGI
                     YLARMERVSPIYLQIQQIEQKAKELEERRETEASTAAKTLATKLRLEIKSYLDNNESD
                     EKSALNSFKINAKRHIENSKETLNQHREEWKYLLANVTLGVFLLGIGYLAAILINKAT
                     TGNYTFFSQTNSGKKLDALEKAISSTHSETLVYG"
     gene            complement(12765..13328)
                     /gene="thpR"
                     /locus_tag="AC06IO_001444"
     CDS             complement(12765..13328)
                     /gene="thpR"
                     /locus_tag="AC06IO_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIO9874639.1"
                     /translation="MNAIRAFFAISLPPAIQENIRSNLEPLRQMAPVHSIRWTKPMHL
                     HITLQFLQRLETKHIQILIERVSNELNHIKQFDLEFGVLEWFPSPKNPRLISLSVGPQ
                     DRLRDLSRMIGQAIGGLNYPVEKRPFRGHLTVGRVVNFNPKLQQLLQIKFPIIPPVPI
                     NKFYLIESRPDKEGSNYFPLAQFDLTR"
     gene            13590..14576
                     /locus_tag="AC06IO_001445"
     CDS             13590..14576
                     /locus_tag="AC06IO_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIO9874640.1"
                     /translation="MHAMIMEKPGCALQYQEVNKPNPNKNELLIKVSACGICRTDLHV
                     VDGELKNPKTPLIPGHQIVGTVEQVGAAVKKFAIGQRVGVPWLGGSCGICNFCTSGRE
                     NLCDQACFTGYQIDGGFAEFCVANYHFCFPIPDGYPDHQAAPLFCAGLIGYRALLKAG
                     DATHLGLYGFGAAAHILIQVACQKGIKIYAFTSPGDQETQKFAYRLGAVWAGDSTQSP
                     PHSLDAAIIFAPVGALVPQALSHTVKGGTVVCAGIHMSDIPSFPYEILWGERTVCSIA
                     NLTRKDGEEFLSLAPKIPVKTKVQTYPLTEVNRALDDLRHGRYRGAGVIVMK"
     gene            14839..15333
                     /locus_tag="AC06IO_001446"
     CDS             14839..15333
                     /locus_tag="AC06IO_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIO9874641.1"
                     /translation="MGSLRKHQPGKTTDLLMSPFLRLQQEVNHAMSDFYNLFDPQRSA
                     NLEQFEHISLTPSLDIVEDKDNFKIEVEMPGMGEEDIKVSFCENRLTIEGEKTTSKKD
                     ENKNYISREISYGRYERTISLPLSADVDKATASFKKGMLWITIPKKTEARENVKTIKI
                     EKAK"
     gene            15699..16394
                     /locus_tag="AC06IO_001447"
     CDS             15699..16394
                     /locus_tag="AC06IO_001447"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIO9874642.1"
                     /translation="MTMKFPFQIIDLTHPISPDSPSWDLDCGFTHSNFIDYEDCTSEI
                     KFRVQQIHSPLGIGTHMDAPAHCISGAASITDLDLHRNYLLTQSVLINVSHQTDENYQ
                     ITKQDILDFEARYGRLKKNVFVLFYTGWEKHWPNPEKYRNNLVFPSISAHVAQLLLER
                     NIAGIGIDTLSPDTADSGYPVHQLLLQAGKYIIENVANAHSLPPIGAWTLALPWKIIN
                     ATESPVQLIGLVR"
     gene            complement(16433..17851)
                     /locus_tag="AC06IO_001448"
     CDS             complement(16433..17851)
                     /locus_tag="AC06IO_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HIO9874643.1"
                     /translation="MDKNHNTKKILFKNRFVILGFGCVGQALMPLIFEKFDIKPSQVT
                     IIAAEGTKIDVAQQYGVSFKLQQITPQNYLEVIGSTLEENDFLIDVSIGISSLALIIL
                     CNQKGALYINAATEPWKEEFVMEKMALNRRTNYSLREEVLRLKDKTQKTALITHGANP
                     GLVSHFIKEALLNIAKDNGLTINRPKNAAEWANLAMTLGIKVIHVAEQDSQVTYPPKS
                     PGEFVNTWSANGLILEGLQPAEIGWGTHEAHWPHDAYSHSNGPQCAIYLSRPSAGVMV
                     RSWTPTLGAFHGFLITHAETISLTNFLTLKNGSELLYRPTVHYAYNPCPDARLSIFEL
                     KSNEWKPQNKNRLILNEIIDGCDELGVLLMGNQRGAYWYGSTLSIQEARQIAPYNNAT
                     SLQVVASMISGIIWAIEHPDEGIVEPEEVDHQYIIDIAKPYLGKVGGYYTDWTPLKSR
                     GELYPEEVDLSDPWQFFNIRVN"
     gene            18128..18577
                     /gene="trmL"
                     /locus_tag="AC06IO_001449"
     CDS             18128..18577
                     /gene="trmL"
                     /locus_tag="AC06IO_001449"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIO9874644.1"
                     /translation="MIHIALYQPEIPPNTGNIIRLCANTGAQLHLIHPLGFTLEDKQM
                     RRAGLDYHQWASILHYENWQTFLQSHSQRRLYCCSAKGKVKYSDVDYKADDILLFGPE
                     SCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNI"
     gene            complement(18736..19386)
                     /locus_tag="AC06IO_001450"
     CDS             complement(18736..19386)
                     /locus_tag="AC06IO_001450"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIO9874645.1"
                     /translation="MLDVINFKSYRSILCLNGDLPDPSFFHKRKLPVIAADGAANVLC
                     NLGVFPDLITGDLDSIQPALLENHSFLHLPDQGSTDYQKAMHYLQTHDLLPAIVVGIN
                     GGYLDHILNNINIFMDTNCLLYSPPLKGFVVNEKLRVNFILPVQTKISLIGIPEVVLS
                     SEGLKWELKHSHLSFPGKNSCFNRTQTAEISLEVHQGAALVLIYEDAIEDAGVSSL"
     gene            complement(19370..19636)
                     /locus_tag="AC06IO_001451"
     CDS             complement(19370..19636)
                     /locus_tag="AC06IO_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HIO9874646.1"
                     /translation="MSIVIFSGIVAFITSFIGLLPQIVKSLKTKSTEDLSMMMLVNYL
                     VCSLAWIIYGSSTNSFFVTSSNVVGLIVSSLLILLKRRYDARCN"
     regulatory      complement(19623..19726)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            19868..20812
                     /locus_tag="AC06IO_001452"
     CDS             19868..20812
                     /locus_tag="AC06IO_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LirA/MavJ family T4SS effector"
                     /protein_id="HIO9874647.1"
                     /translation="MTITHTTAGGITMTVELIRNGETDTNDYEEHLDSFGYETLEKSL
                     KEDAKSICQFFCNEELVLQGLKKLVTQFYQFNQPEKKTLRQFFDDWGSKNHFNHEAQP
                     NDKIPYETSPNFHFPNHTPILHGELTADLFNNVLLKQGYLSADLGAGPKHGKWAHTIQ
                     FFILEEARKTGQLKLHSNTVCEFIKKISQIHGPYESFSLWSILFDSFDETFTCPNIIT
                     QTLTSPWDNSKEALFLADKLNHFQKKFEKAASAENNYNAYANKKYMSRIDKANYVKYK
                     DKCAILWFAPQDKKTIPKPSCQEELKQNQLSFLKLSLL"
BASE COUNT         7097 a         4180 c         3777 g         5934 t
ORIGIN      
        1 ttattttgct cacactgggg aattaaatgg ccaaaactat tacaaaagac agtttttttc
       61 atagtcgttt acaacaattt gtagctaatt ccctttttcc agcaggggcc ggtgattggt
      121 ataaaaacaa aggctacgga aaaaagggcg aagttgatga cgaagctcct tttagagatt
      181 tcgttgaaaa acaaaaaacc gataaaaaat cacactatta taaagagttt caaggcctgg
      241 atttaagtct gaagaaagtg aaaacaaagc tggtttctgg tgaatcctgt cagttagaag
      301 tcatgaaatg ccaaccagag tctcatgaac ctaaaaagcc tggcaccgga aaacacattg
      361 tctattttcc cggagcaaat acttattacc aagcctgttt tcgtgatatc agcacagctt
      421 gcaaagaaac tggagcaaca gtacatgctt ttaattttcc aggaacagga cttagttcag
      481 gaaaggtgag agaggcaaat gacttaatca atgccggtat ttctgtggta tcttctttga
      541 ttaaacaagg cgtgcacccc gatgatatta ttttacaggg cgattgctac ggagcaagca
      601 ttgcattaga ggtaaaaaag caattagaag accaggcaga catcaaagtt cgtgcaatca
      661 tgaacaatgt tttcaagtca tttaaagcag ccgtttgcga tatgattacc caatctccat
      721 ggctacctaa tatattaaaa agcattgtta aaagattgtt agaatttact ggctggcatg
      781 ttactccggg aaaaaaatat aaacatgccg atccttacca atgtcatatt caacacttgg
      841 gagatcaaac gctagaatcg tcaacattat caggtaaagt cagcaaatat catcatgaaa
      901 tacaaactgg gcaaaccaaa tctcaaaaaa gggcccccat taccgatact tgtccagaag
      961 agtatcgaga ggatcgcgat gagttggata ggaaacatta tgttcgagtc aaagaaagcg
     1021 ctaaagaacg ccttgcttcc aaatttggag tagataaatt tggaagggtc aatgctcact
     1081 ttgccgattt atgcgaactg gaaatgttgg atgggcaatc ggtgtatcaa ggctttgcca
     1141 atgactatat tgccagatcg aatgcttata tcgaaaaaca ccctcaaaaa ggtattaaag
     1201 aaattcagga tgatttacgt aaattacatt acctgcagcc agctgattca atagaaatta
     1261 ctgaagatga ggcccaggat tttaacacgg tagttgatct tattacagat gaacaacaac
     1321 taaggcatga tcgagtcaac gacaatacaa taggtaaaag catctctatg taattgaaag
     1381 tactgactgg caggacgatt cagcttttgc cagtcagttg cctcgaacaa aatccttgtg
     1441 cttcaacgcc aagggcacta ttaaatttac tggacatatt ctgaaatgca atgagtgctg
     1501 tcaattcaat gatttcctgt tcagaataat acaatttcaa ttgattgaag cactcatcat
     1561 caggacgctg atcagaataa gttacttttt cagcataatc caatgccgct ttttcttctg
     1621 gtgaaaataa tgaggaactc ctccatttcg ccagttgttt atactgtgct tcacttccag
     1681 cccttttaat taaagttgct gaattaatat caacacaaaa cgcacaccag ttaatttgag
     1741 aaaccctgac agttatgaga gagcgtaata ttggggaaag tggtgatttt tttctgtcta
     1801 tcacgccata catagcagca actgccaaaa ataacctggg aattcttgcc caaatcaaag
     1861 caggttcgag tatttggccg tattttcttt tttgattcca gaagaatggt tttaaatacc
     1921 agggagtctt cttgatattt ttttccaaaa ttctcatgca taattatcct ttttatgaat
     1981 actgaattaa tagcatggaa taagaatgaa agaaattatt ttggcaacca gtaaccctgg
     2041 aaaaataaaa gaattagaac aattactagc ccccacccta tgcattcccc aagccgactt
     2101 gggtatatcc gatgcggaag aaacaggatt aagttttata gagaatgcca ttctcaaagc
     2161 ccgtcatgcc agctcattag ccaataaacc agctttagcc gacgactcgg gacttgtagt
     2221 gccctcttta aatggagagc cgggtatcta ttcagcacgc tatgctggaa taaaggcaaa
     2281 tgacgaagac aatattcaac aattgttaag taaaatggct gatttatccc aagaacaaag
     2341 acaagcctat tttttttgcg caatagcgct aatgcaacat gccaaagatc caacaccgct
     2401 cattgccaca ggcgtatttc atggcgttat tagcgtgaaa ccttcaggaa caaatggctt
     2461 tgggtatgat ccggtatttt atctcaacga atatcaatgc acagccgctg aattacctgc
     2521 taaaattaaa aataggatta gccatcgcgc aaaagcttta aaccaattac gcgcccttct
     2581 tccagattaa tatgaacagt gaagtagata ctctttttgc acaagcctat aagcttcaat
     2641 atgaaggcca tttacctcaa gccattagct tatatgagca gatacttgcc caatcaccca
     2701 aacatacgga aactctgcat ttcctgggat taacctatgc ccaattgggt gatatggaaa
     2761 atgccatttt atattttttg caagccagaa aaattaaccc caatgacgtt ggtatactca
     2821 ataatctcgc taacgcttat aaaaaggcag gtcaattaga tgaggcaatt aaatactatc
     2881 aggaagccat tgagattaaa ccagagtatg tccaggctca taataatctg gcagcaacct
     2941 acgcactgct taacaattac cagaaggcct tgcatcatta cgtgattgca gtgaatacag
     3001 aacctgactt tagtgcggct cactttaatt taggtttgtt acttctcaaa aatcaacaat
     3061 tgtctgctgc taaaacacaa tttaataatg tgatagctct aaatccccag cacagagaag
     3121 ctcaatttta tttaggtatc ttgcatttgg aagacaatct acttgcggaa gcagagcagg
     3181 catttcataa agtgttagag caagatcatg agcatgtcca atccctaatt aacttgggcg
     3241 tgatcgcttt gaaaagagaa caaaatcaat tagctgttga ttattttacc aaagccttgg
     3301 ctttggataa tgaagacatt gatgcacgta ataatttggc agctaccttc atgcatcacg
     3361 atcgatttga aaatgcgctc atgcattacg atgttttact gaaaaaagaa cccaataatc
     3421 tggaatattt atacaattcc ggagtcgctc aaatggcatt gggtcatctc aatgaagcca
     3481 cccttttatt cgatcagatt ttaacattag aaagtgatca cactccatcc ttgaataatc
     3541 tggccgctat ttatttaaaa atggacatgc gagaaaccgc gcgcgaatat cttgagcgcg
     3601 ctctggcaat caaccccgat gatgtggtca gcaggcatat gctgaatgcc attacaggag
     3661 caacgagcgt taatactaca gaaaaatatg cccaaaattt atttaataat tatgccttgt
     3721 attacgacca acacatgcaa ggtgaattac actataagat tccgcatcac attggccgcc
     3781 tcatccacca acttcaactt ttgcaaacca gtcattcttt ggacttaggc tgtggtactg
     3841 gattaacagg tattgtgctt cgtgaaataa gcaaacactt aaccggagta gatatagcag
     3901 aaaaaatgat agctcgagca aaggaaaaaa acatctatga tcttctggta tgctcggaat
     3961 taatcgattt tttaagaaaa gataaaaacg attacgattt agcggtcgct gcagacgtcc
     4021 taccctattt tggtaatctg gatgatattt ttaattccat caatcaacat ctgaagcaag
     4081 aaggatattt tattttcact accgaaatca gcacaacaac tccgtggaaa ctggaaagca
     4141 gcgctcgatt tagccatcaa cccgaataca tagagagctt aatcaataaa taccaatttc
     4201 atcttatcaa acaagagaaa ataccaggcc gaacacaaaa taaacaagtg ctggaagtca
     4261 tgttgtattg tgttaagaag tcagcatctc gttttgttgc caacagaact tacgaaaacc
     4321 cctaatcact ttgttaaaaa acaaccgttc acaggatatg taattaggga atgccaagtg
     4381 ttctgtcttt cgccttcgcg ggaaggacaa ctcaaataaa taggattata aacagaaaaa
     4441 actgcattcc caatgaacgg ttgtattaaa aaataaactt ttatctaggc tttttcgtta
     4501 gtcctgtcca tatctgacaa attctgagca gaataccact atattgaatt tttcatattc
     4561 ctaaacacta aaatggcaaa tccaactcag aatccaggcg ctgaatttgt ttcttaaaca
     4621 aatattgaat ttgctccaga tgatctttat ctgttgcctc aaaacgtgcg accagacaag
     4681 gagttgtgtt tgatgctcgt aataatcccc atccatttgg aaattcaaca cgcaaaccat
     4741 caatattcaa aatcctgccc tcagggaaat cagcctgctc actaaaccgc tgaataaaat
     4801 caaatttttc ttcatcagta atcgctattt ttatttcagg agtattgaca ctattaggga
     4861 tcaactcaaa ttgttcgctg acagtcaaat gggaagagct gataatctct aacaatcggc
     4921 acgcgctata taaagcatcg tcaaatccat accagcgatc tttaaaaaac agatgcccgc
     4981 tcatttcgcc agccaggatg gcttgttctt ttttcatcac cgctttcaca attgaatggc
     5041 cagtggggca cattctggca acaccgcctg ctgcctggat ttctttctcc aaatgactgg
     5101 aacacttcac atcaaacaca acagtagcac ctggattacg acttaaaatc tctcgtgagt
     5161 aaaacatcat taaacgatca ggccaaatca cttcgccttt atttgtcact agaccaagtc
     5221 tgtcagcatc tccatcaaag cccaaaccaa tatcagcttg atgatttgcc acagccgctt
     5281 ttaaatcaac taaattagct tctattgaag gatcaggatg atgattggga aatcgaccat
     5341 ccacttcaca atacagggga atgacttcac agcctaattt cgcaatagcc ctgggaataa
     5401 ttggacccgc cacaccattt ccacaatcaa caactacttt taaagggcgc ttcaattgaa
     5461 tatcgctcac gatacgctga atgtaacctt ctatcacatc aaaagccgtt tctgtgccac
     5521 gaacagccga ctgtttgact tcacccagca aatcgtaaag tgtatctatt tcatcttgaa
     5581 ttaatgtttt tccagccaat actaacttga taccattata atcagcagga ttatgacttc
     5641 cggtaaccat caatccagaa tcaatccctt gcgtatgggt agcataatac atgacaggtg
     5701 tcgccacagc accaagatca aggatatcta tcccgctgtc tagcaaaccc tctttcaagg
     5761 ccgtagccaa tgcataactg gtcagacgtc catcacgagc tacaaatatt tgtcgacgat
     5821 ttaatttttg taaataacgt gcaatagcta agccaatgct ataaaaagaa ttctcatcca
     5881 gttctttacc gataatcccc ctaatgtcat aggcacgaaa gacagagcgg gggatttgtt
     5941 tttgttgata ggtcatttaa tatctcccgg agctgccaaa accaccttct cctctggagc
     6001 tttctgtaaa ttcattgacc acctcaaaag aagcctgcac aaccgggata aaaactaatt
     6061 gagcaattct atcaccagga ttaacagtaa aatgttcctg gcttctgttc caacaagaga
     6121 tttttaattc tccttgataa tccgagtcaa tcagccctac aaggtttcct aaaacaatac
     6181 catttttatg gcctaaacct gatcttggca aaatcactgc cgccagcttt ggatcggcaa
     6241 tgtaaatagc aatgccggtg ggcagcaata ctgtttgctg aggtgctact tgcataggct
     6301 ccgagataca gacccttaaa tccagaccag cggacccatc agtggcatag gcaggcaatg
     6361 gaatggtatc tcctatcctt gaatccagaa tttttaactg aataacttga tgcataataa
     6421 ttctcaatcg ttttattgcg cctaattttg cagacttgca gcaaggattg caataatttg
     6481 gcctgccagg cgtgttttat gggttaatgg cagctctatt tgtttgtttt ttgtaatcac
     6541 tgttacttga tttacatcac tttcgaatcc cattccccta cctacctgat tggcgataat
     6601 catatccagt tttttatttt tcattttttc ttttgcatag gaaatcacat cagttgtttc
     6661 agctgcaaac ccaacaacaa aggaagcttt accactttta gctacttcag aaagaatatc
     6721 ggtatttcta acaagctgga gagtgagttc agaattctca ttttttttca ttttttccga
     6781 agcaggattc agcactttat aatctgatac tgctgctgtt ccaataaata catctccctg
     6841 ttgtacatgc ctgttcactt cttccagcat cgattgagca gactccactc ttatgacctc
     6901 gatgtccgaa aacgtttgta aatgacaagg accactaatt aatgtcactt gtgcgcctgc
     6961 catagcggcg gcttcagcca ttgcaaaacc catcttccct gaactgtaat tgcttaaata
     7021 acgaactgga tcaatcgact cgcgagtagg gcctgcagta atcacaattc gtttgccatg
     7081 caatagttga ttcacatcca gtaagcgcaa ggtattcaca atttgatccg cctcgctgac
     7141 tcttcccaat ccatgttccc cacaagcctg ggagccttct tcaggcccga caaaaaacac
     7201 acctcgttct tttaacaatt cacaatttgc cttggttgct ggatgtgccc acatgcttct
     7261 gttcatggca ggacaaacaa ttactggcgt ttcagtaacg agatataatg ttgaaagcaa
     7321 atcatcagca agaccttgag ccattttagc aagacaattt gcggacgcag gagcaatgag
     7381 caaataatct gcccatctgg ctaattcaat atgtcccata gcccgttcag cttgagtatc
     7441 gaataaatca atcctcactt cattgcctga taatgcctgc atcaataaag ggttaatgaa
     7501 ctgttgtgct gaatgagtca tgacaaccct cacatcagcc cccaaacgag tcaattctct
     7561 aaccagatga gcagatttat aagcagcaat gcctccacaa ataccaagta gaattttttt
     7621 attaataaaa tcttgcatgg ctcgccattt ttgataagaa taactccgat caaagcacaa
     7681 acttcgcaat aaaggaatat aaaaggaata caaaatgatg gttgcccata cagcgcaaca
     7741 acttgacttg cgcgaaaaat tacttactaa tggagtacat agcctctctg atatcgaatt
     7801 gttagccgtt ttcatcagtt cgggtaacaa taaaaaatct tgtttgcaac tagcctatga
     7861 gctcaccaaa cacttgggaa atttacggaa tatcctcaat gctgatctgc aaagctttaa
     7921 atcaatccat ggattaggag aagttcgtta tgcacaactt caggctgcga aagaaatatg
     7981 tcatcgtagt gatttcattg atctgcaaaa agaaatccgg ttatctaaca cgcaacaaac
     8041 ttatgctttt ttaaaaaagc gactgcgaga ctacaaaaat gaaacttttg ccgcactctt
     8101 tctggataac caacaccgaa tcattgctta tgaagaatta ttctcaggaa ccatcaacac
     8161 agcaacagtc tatcccagac cgattgtaga acgtgtattg caattaaatg ctgctgcttt
     8221 aattctggca cataatcatc cctcgggctt gtctgatgca agccagcaag atctagccat
     8281 tacaaaacgc attcgagatg ctctggactt ggtagatgcc cggttactgg atcacattgt
     8341 cattggggat aatgaagtgt attccatttt tgctgagaac aaatgggtat gtaactagtt
     8401 cccgccccta tcatttgagt taatggatta aaaatgttcc aaccatttat tacagatttt
     8461 ataaaaaatc gttcgtttca atttattttt gtaatgggat tagtttctga tatcgccctg
     8521 gctgggacga taaagcacat aatatttctc tttgggttcc cctatttaag ctctgttggt
     8581 ctttattgtc taatgaaccg taaaaaaaca actgttaaag caagaatcag tgagcaatac
     8641 cggccaaagc agaaagaaat ttgcagatga attcttaagg ttttcttaag gtttagttaa
     8701 tacttggtgg ctataatggc acaaagtatt gttttaggtc agagtcatta tgttaactta
     8761 tcaaggtaaa gaaatagttc gatttaaaga aaaaacaggt ggtaaaaata aaagtgatgt
     8821 cgatggtttt tacaaagaca gcgatggccg gaaatttttt ataaaaaaac cgggcgaccc
     8881 tcgtgagcta ttcacagaat tatttgcagg cttgctatta aaagagttca tgaagcgagg
     8941 attgattgat gaaagctatt ttccatcatt gatttgcgca gatgtcattc aatttgaaga
     9001 caaatcctat ggcctgatac agccactggt ttcatttgat gaattacaca aaatcattgg
     9061 aacaagctac ggtgatggca gtgatcgtaa tacgataaag gaaaccttct ttgggccagg
     9121 ttattatgcc gggatcacaa aacaaaataa atattttggc ctttctatgg cccttatgtt
     9181 ttctctattg ctgggagccc atagtgtaca tagtggcaat atcgttgtac tcaaaggaga
     9241 aggagaagga gaagaaaaaa gcaaacaatt tggccgaatc gattggggag atgccttccg
     9301 ttatttcgct catcgaaaca ataatgacaa tctcctttat gcttatgaaa acagaggttg
     9361 gtttaattat aaatcactaa ctaaagatta tttcctgaac tataagaaga tcaatggcct
     9421 cttccctgcg atggctgaaa aagcccgaca attgcaatcc aaactaaatc ctgaattgct
     9481 tattgaaata gtaactagcg cactcaaaaa tattccagct gatctgatag aagaaaaaac
     9541 caaaatccaa cttgctgcct acatgtgtat ggattcattt aaagaggtaa catttggccc
     9601 aaagggtaat tgcaaagatt ttgccattgc catggccaca ttattagaaa atcgtatagg
     9661 aaaaattgct gttcttaaag atatgtcccc attgagcaat cctgaagagc tgtatcaaag
     9721 tattttggaa ttaaaaccct tgacgcttct tatgacctct tcaacctctt tttcagaaac
     9781 cataaatcaa tgggcagata tcttaaaaac tacagacatg gaaaaatttt cgtttgatag
     9841 taatccaatc aacctaccgg aattagtcaa acaatttaac ctttacgttg atgaactagc
     9901 catcacgtgt gaagccaata atgtctgggc aaaagaaaga atagacagca cccctaatct
     9961 gtttgctctc tatgataata gcggtggtga agcgattcac ggacatgcct ttgttcctta
    10021 ctacaaagaa agcattgtat tgcgccgcct atttacagta gaccctaata ctttcaactt
    10081 atctcgtttt gctgcttttg aaggaccatg ccaactctat tgcaaggaac ataaagactc
    10141 tgcctgggtt aaaatccaaa ccttactgac tttaggtaat ggaattatta atacgctaaa
    10201 aataatcaaa caggctcaag cattcggaat agatgaggca gttacagaga atttaaaagc
    10261 attgaaagaa caattcattg cttttcaact tgcggaagct gatgtaaaag aatcattaaa
    10321 agcaccttct tttgcagagc ccttgcctga taaggaaagt gagttttttt atcccattga
    10381 tgaaaaagca ttagctaaaa tgaatggata tcaacttgca acgatttgtc tggaagaact
    10441 aaatagccct aaaccgtcgc ctttaattga aagaattctc agtaataaaa aattctggaa
    10501 acgtattaac tctgcttttg aaagtggtgt atttaaaggc cgcacagata atcccgccgg
    10561 taaaatagct aaaatccatg aatggcatca attattacag atttctggta aaaaaactgc
    10621 tggacaaatt gatgagctgc aaaagatagt aatttctctt caatcaaaaa ttaaaaagca
    10681 aacgattgaa tttgaggagc tagaagcgac tttaatccaa ataaaagaaa aatatcagtt
    10741 actggagaaa atggctgagc aaagcgaaca tgagaaatct tccgcccaat ccattattcg
    10801 ctctctgaat ttagaactgg gtcagcttaa attacaactg caagagcaag aaaaattaca
    10861 gttccaatta aaagaactca aagagaaaat acatgaacaa acgactctat ccaagcgatt
    10921 aggagaggag ctgcaaacgc aaaagaaaac caacacgcac caggaagaaa ccattcaaag
    10981 aatcaccaaa gaaaaatcat tggctgattc ttcccttgag tcgcttcgaa aagaactgca
    11041 tgaattggca aaaaaagaac gctctcttca taaaacgctc gaagaaaaac aattacaagt
    11101 acaagagtta gaagagcaat tagcagagaa agaaaaggaa aatctagccc tgaaaaaagc
    11161 tgacaaacaa agtcaacatg aaaaatccct ggataaatct gctattgaat ctctaacttc
    11221 agagcttaat cagcttaaat tagaactgca aaaacaagaa acattagaat tgcagttaaa
    11281 atctttaaga aaacaaattc aagagcaaac attacttgta gaaggcttga aagaggagtt
    11341 acaaaaacaa aagaaaagca acacacacca ggaagaaact attgaaagaa tcactaaaga
    11401 aaaatcattg gctgattctg ctcttgaatc gcttcgaaaa gagatgcatg aattaactcg
    11461 aaaaaacgaa gaaaatcaac ttaaattaac aaaacaagtc cactctctta gcgaacaact
    11521 tgaagagaaa caattacaaa ttagagaatt tgagaagcaa ttacaagaaa aagaaaaaag
    11581 agtcgagcaa agtgagaaag agaaagcttc cgaaaaacga actgtggctt cacttaggga
    11641 gcaagtaagc aatttaaaat tgcaattaca gcaacttgga gaggtaattc aggaaaaaga
    11701 aaaaggctcc tctttgataa gccagcaatc caaacaaatt attgctcttc aagagattgt
    11761 agaagagcaa aaaagacaat tggaagaatt gaaaataaaa attcaggaac ttgtatctgc
    11821 aaatcaagag cttggaaaac aaaatcaaag tttgagcaag gaaaatttac ataataagaa
    11881 tacagttgaa gatcttaaga aaaaattgac cgaattaaat gttcaattgg aacaattgca
    11941 tcaatcctca agtgaaaaag aacaaaccat tagaaaattg agagaagaac tcattaagaa
    12001 agattcttcg ctcaaacaaa atgaggaaat gcaattagcc caaaaacatc tgcaagagga
    12061 aattgatcgt ctacaaaaag aaattaaaca acaacaatta aatacaaatc aattagaatc
    12121 tataattgct caaagtaaag aggctgaaaa acgctatcag caagctttac aacagaaaaa
    12181 gggaatttac cttgcacgaa tggaaagagt gtctcctatt tatttgcaaa tacagcaaat
    12241 tgaacaaaag gccaaagaat tggaggaaag aagggaaaca gaagcatcaa cagcggcaaa
    12301 gacactcgca acaaaacttc gtttagagat aaaaagttat ctcgacaata atgagtccga
    12361 tgaaaaaagt gccttaaact cattcaaaat aaatgccaaa cggcatattg aaaactcgaa
    12421 ggaaacgcta aatcaacatc gcgaagaatg gaagtacctg ttagccaatg ttactttagg
    12481 ggtattcttg cttggaatcg gttatctcgc cgctattttg attaacaaag caactactgg
    12541 caattatacc tttttcagcc aaactaacag cgggaaaaaa ttggacgcgt tggaaaaagc
    12601 gatttcttcc actcactccg aaacattagt ttatggataa tcttctcttt ttggcatagg
    12661 ttggcttaaa ttcatcctat gccaaataat taaagacact tgagcataat aagttggaca
    12721 cagaggcaaa gaatcgttcc aattggcaaa tcagccattc tcttttaccg ggttaaatca
    12781 aattgtgcca agggaaaata attacttcct tctttatcag gcctactttc aattaaataa
    12841 aatttattaa tcggaactgg tggaatgata ggaaatttta tttgcaacaa ctgctgaagt
    12901 tttgggttga aattcactac tctacctaca gtcaaatgcc ctctgaaagg acgtttttct
    12961 acaggataat ttaaaccacc tatcgcctga ccgatcattc gagacaaatc ccttagtcta
    13021 tcttgtggac ctacagataa tgaaataagt cttggatttt tgggagaagg gaaccactct
    13081 aaaacaccga attccaaatc aaattgcttt atatggttaa gttcattact tactctttca
    13141 atcaagatct gaatatgttt tgtttccagt ctctgtaaaa attgcaaggt gatatgcaaa
    13201 tgcattggct ttgtccacct tatggagtga actggtgcca tttgcctcaa tggctccaaa
    13261 ttactccgga tattttcctg gatcgctggt ggtagtgaaa tagcaaaaaa agcacgtata
    13321 gcgttcataa tatgagttgc ctattggaaa aatattgcaa tccacaaaat aaattccgaa
    13381 aaacaaacgc tttttccaaa ttaattatca agatcgctgc tgtttttcaa ttttttgctg
    13441 cctgtcaacg accccgatta atggcctcca ggcaagctat tcattcatca gcattgatgt
    13501 caaatattgt tactttttga atgcattaag taagtataac ccaccctact ataattgtaa
    13561 gatagaggat aaaaacagga ccaataccca tgcatgccat gatcatggaa aagcctggat
    13621 gtgcattgca atatcaggaa gtcaataaac ccaatccaaa taaaaatgaa cttttaatta
    13681 aagtatcagc ttgtgggatt tgtagaactg acttgcatgt tgtcgatggt gagctgaaaa
    13741 atcccaaaac tccccttatt ccaggccacc aaattgtagg aactgttgag caggttggag
    13801 ccgctgttaa gaaatttgca attggacagc gagttggagt cccctggtta ggtggcagtt
    13861 gcgggatctg taatttttgc acctctggcc gagaaaattt gtgtgatcaa gcctgcttta
    13921 ccgggtatca aatagatggt ggtttcgctg agttctgtgt agcaaattac catttttgct
    13981 ttcctattcc cgatggatac ccggatcatc aggctgcacc attattttgt gctggtttaa
    14041 ttggatatcg tgccttgcta aaggctggtg acgcaactca tttaggtcta tatggatttg
    14101 gagcagccgc acatatactg attcaagtgg cttgccaaaa aggaataaaa atttatgcgt
    14161 ttacaagccc cggtgatcag gaaactcaaa aatttgcata ccgattaggt gctgtctggg
    14221 ccggggattc tacgcaatca ccacctcatt ctcttgatgc agcaatcatt tttgcacccg
    14281 ttggtgcatt agtgcctcag gctcttagcc atactgtaaa aggaggtact gtggtttgtg
    14341 ctggtataca tatgtcggat attcccagtt tcccttatga aatactatgg ggtgagcgca
    14401 ctgtatgttc aattgccaat ctaactcgca aagatggaga agagttcctg tctctggccc
    14461 ctaaaatccc agttaagaca aaagttcaaa cctatcccct taccgaagtt aatcgagcac
    14521 tggatgattt aagacatggc agatatagag gagccggggt tattgttatg aaataaaact
    14581 ataattatca aaagctatta caacaaattc aaatcatcaa ggaagtaact cgcctcgctc
    14641 aaaattggtt taagcaacac ctaaaaagaa taaaataaat acaaaaacaa caacataaaa
    14701 tcattgtgta ataaatttac ggcaattgaa aatattttat ctagcaacta tatttacatt
    14761 gataggctgg tgaatattac tttcggtgtt tttaacaact aactgatacg tcaatttatg
    14821 gaataaggag gtgacatcat gggcagctta agaaaacatc aaccaggtaa aacaactgat
    14881 cttctaatga gtccgttttt aagacttcag caagaggtca atcatgctat gagtgatttt
    14941 tataatttat ttgatcctca aagaagtgcc aatcttgagc aattcgagca tatttctctt
    15001 actccgtctt tggatatcgt tgaagataaa gataatttca aaattgaagt agaaatgcct
    15061 ggtatgggcg aagaagatat taaagtatct ttttgtgaaa acagattaac cattgaagga
    15121 gagaaaacca cttctaaaaa agatgaaaat aaaaattata tcagtcgtga aattagttat
    15181 gggcgatatg aacgcaccat ttccttacct ctatctgcgg atgtggataa agcgactgcc
    15241 tcttttaaaa agggtatgtt atggatcaca atacctaaaa aaactgaagc cagggaaaat
    15301 gtaaaaacaa ttaaaattga aaaagcaaaa taactaattc aaccagggtc aggctccaaa
    15361 gcgatggagt cagccccgtt cttatttttt ggctctactt tctcaaatgg tagttaataa
    15421 cataagaatt atgcctatga gagcccctgt tactgaccca atacccggtg aatttaaact
    15481 tcctatgaca gcgcccaaaa taattccaat ggatatttca atactaatcc ataattatgt
    15541 aaaatttgaa tacaattgtt aattattagc ttgaatgatc attattataa taaaaagaca
    15601 aactgacact cttttgcaaa acctaaatta aaaattagtt tcattgatcc tgattctttt
    15661 tatgataatt taattgaaat aatccatcgc tctctctaat gactatgaaa tttccttttc
    15721 aaataatcga tttaacacac cctatttcac ctgactcccc tagttgggat ctggattgtg
    15781 gatttaccca tagtaatttt attgattatg aagactgcac atcggaaatc aaattcagag
    15841 ttcagcaaat acactcaccc ttggggattg gaacccatat ggatgctccg gcccattgta
    15901 tttcaggggc cgcctctatt actgatctcg atctgcaccg taattacctg ctcacacaaa
    15961 gtgtgctgat taatgttagt catcaaacgg atgaaaatta tcaaattaca aaacaggata
    16021 ttctggattt cgaagccaga tatgggaggt taaaaaaaaa cgtgtttgtg cttttttata
    16081 ctggttggga gaaacattgg cctaatcccg aaaaataccg caacaatttg gtttttccaa
    16141 gcattagtgc ccacgtagcc caattattgc ttgaaagaaa tattgctggc attgggatag
    16201 acacactttc tccagatact gcagactcag gttatcctgt gcatcaactc ttattacaag
    16261 caggtaaata cattattgaa aacgttgcca atgcacattc cctgccaccg attggcgcct
    16321 ggacccttgc tttaccatgg aaaataatca atgctactga atcaccagtc caattgattg
    16381 gactggtgcg ataatttata gttcatcgat tattcagtag gtttgcttcc ttttaattaa
    16441 cccttatatt gaaaaattgc cagggatcag ataagtccac ttcttcagga taaagttccc
    16501 ctctactttt taaaggggtc caatccgtgt aatagccgcc gacttttcca agatacggtt
    16561 tggcaatatc aataatatat tgatgatcaa cttcctctgg ttctacgata ccctcatcag
    16621 gatgctctat tgcccagatt attcctgaaa tcatactggc gacaacttgt aaactggttg
    16681 cattattata gggggcaatt tgccttgctt cctggataga aagggttgaa ccataccaat
    16741 acgcccctct ttgattaccc atcaacaaca cacccaattc atcacatcca tcaatgattt
    16801 cgtttaaaat caggcgattc ttgttttgtg gtttccattc attacttttt aattcgaaaa
    16861 tggagagcct ggcatctgga caaggattat aggcgtagtg aactgttggc ctatacaata
    16921 attcagatcc attttttaat gttaaaaaat tggtaagtga aatagtttct gcatgagtaa
    16981 ttaaaaaccc gtgaaaagca cccagtgttg gcgtccatga acgcaccatt acaccggcac
    17041 tagggcgaga cagatagatc gcgcattgtg gtccattact atgagaataa gcgtcatggg
    17101 gccaatgagc ttcatgagtc ccccacccga tttcagcagg ttgcagtcct tccaaaatca
    17161 gaccattagc agaccaggta ttaacgaatt cgcctggtga ttttggagga taggtaactt
    17221 gagagtcttg ctctgcaaca tggattactt taattcccaa agtcatggcg agattagccc
    17281 attctgctgc atttttaggc ctgttaatag taaggccatt atcttttgca atattcaaca
    17341 aggcctcttt aataaaatgt gaaacgagac ccggattagc cccgtgagta attaaggctg
    17401 ttttttgagt tttatccttt aagcgaagga cttcttcacg caaagaataa ttggttcggc
    17461 gattaagcgc catcttctcc ataacaaatt cttctttcca cggctcagtt gcagcattaa
    17521 tatacaatgc tcctttttga ttacatagta taatcaaagc aagacttgat atgccgattg
    17581 acacatcaat taaaaaatcg ttttcttcca aagtactacc aatcacttca agataatttt
    17641 ggggagtaat ttgttgtaac ttgaatgaaa ctccgtattg ttgagcaaca tcgatctttg
    17701 tcccttctgc ggcaatgatt gtcacttgcg atggtttgat atcaaacttc tcaaagatta
    17761 aaggcattaa tgcctgacca acgcaaccaa atcctaatat gacaaaacga tttttaaaca
    17821 agattttttt tgtattatga ttcttatcca tgatgaatcc ttaacttccc ggcccagttt
    17881 aatttgaatt tttaatactc aacaactatt caacaatgat catacccaac agacatcaaa
    17941 aactttcata tggcatacta atattgtagt agctgtgttg aattcagaca gcctgttgag
    18001 taaaaaatta gacacatcaa cccctgcttg atttttaaag tatatagtaa aattgcatcc
    18061 tgctactcta ttcctggctt tgaataaatc aattaaaatg gcagccagaa tgctactgga
    18121 aaatcacatg atacatattg cgctttatca accagaaatt ccaccgaaca cgggcaatat
    18181 tattcgactc tgcgccaata ctggagctca actgcatttg attcatccat taggctttac
    18241 tttggaagac aaacaaatgc gacgagccgg tcttgattat catcaatggg cctcaatatt
    18301 gcattatgag aattggcaaa cattcctgca gtctcattca caacgacgat tatattgctg
    18361 tagcgcaaaa ggaaaagtga aatatagtga cgtcgattat aaggcagatg acatcttatt
    18421 atttggaccc gaatcgtgcg gcttacctca acaaatactg aaccaatact caacaatacg
    18481 gattcccatg agagaaaata atcgtagtct caacttatca aatgctgtag caatcatatt
    18541 gtacgaagca tggaggcaaa tggatttcaa catataagca ttaacttggt aatccacctg
    18601 atagcgatta cagcaatgga agagacaggt tatgcaagca gtactctact gtaaacagaa
    18661 cttacgaaaa accacaaggc cgaggcaaaa atatttttta acaaagagat tggggagaga
    18721 gtaagtcctt attccttata aagaggaaac tccagcatcc tcaatagcat cttcataaat
    18781 taaaacaagg gcggcgccct ggtgaacctc caaacttatt tctgctgttt gtgttcgatt
    18841 gaaacaggaa ttttttccag gaaaagaaag gtgtgagtgc tttagctccc acttcaaacc
    18901 ctcagaagac aggacaacct cagggatgcc tattaacgag attttagtct ggacaggtaa
    18961 aataaaatta accctcaact tttcattgac aacaaaacct ttgaggggag gcgaatataa
    19021 aaggcagttt gtatccataa aaatgttgat gttattcaga atatgatcca aatacccacc
    19081 atttatccct acaacgatag caggcaatag atcatgagtt tgtaaataat gcatggcctt
    19141 ttgataatcc gtactccctt gatctggtaa atgaagaaaa gaatggtttt ccaacaaagc
    19201 aggctgaata gaatccagat ctccagtaat taagtcagga aagacaccaa ggttacataa
    19261 cacattagcg gcaccatcag cagcaatgac cggcagcttt cttttatgaa aaaaggaagg
    19321 atcgggcaaa tcaccattaa gacataaaat agaacgatag cttttaaagt taattacatc
    19381 gagcatcata acgtcttttt aataaaatca gcagagaact cactatcaaa cccactacat
    19441 tggagctcgt tacaaaaaat gagtttgtgc tgcttccata aatgatccag gccagcgaac
    19501 aaactagata attcactaac atcatcatgg aaagatcttc agttgatttg gtttttaagg
    19561 atttaacgat ttgcggtaaa agtccaataa acgaggtaat aaaagcaact attccagaaa
    19621 aaataacaat tgacacattt tcctccgctc agactaatga gatcaggttc aaagggttgg
    19681 gtgatcctct cagccaactt atcaattggc acccctaatg ctggacttat attttatcac
    19741 gaaatgagca gaccgcaatt taatagcaac aaaatactcc tttctttttt tatcagttta
    19801 tgattaatta ataatccctt agttcaaaaa aggaatcaag ctcctcaaat aattctcaaa
    19861 tcggtatttg acaataactc atacaacagc tggaggcatc actatgacag tagaacttat
    19921 tcgaaatgga gagacagata ccaatgatta tgaggaacac ctggattctt ttggatacga
    19981 aacattagaa aaatccttga aagaagatgc caagtctata tgccaattct tttgtaatga
    20041 agagctggtt ttacaaggtt taaaaaaatt ggtgacacaa ttttatcagt ttaaccaacc
    20101 tgagaaaaaa acattaagac aattttttga tgactggggc agtaaaaatc attttaacca
    20161 cgaagctcaa cctaatgata agatacctta tgaaaccagt cccaatttcc atttccctaa
    20221 tcacactccg attttacatg gtgaattaac tgcagactta ttcaataacg ttcttctaaa
    20281 acagggttac ttatctgctg atcttggcgc aggccccaag catgggaaat gggcgcatac
    20341 cattcaattt tttattctgg aagaagccag aaaaaccgga caattaaagc ttcacagcaa
    20401 tacagtctgc gaattcatta aaaaaatctc tcaaatccat ggcccatacg aatcattcag
    20461 tttatggagc attctctttg attcatttga cgaaaccttt acctgcccta atataattac
    20521 tcaaactttg acatcaccat gggataattc gaaagaggct ctatttttag ctgataaatt
    20581 aaaccatttc caaaaaaaat ttgaaaaagc cgcctctgct gaaaataatt acaatgcgta
    20641 tgccaataaa aaatacatga gcagaattga taaagcgaac tatgtcaaat acaaagacaa
    20701 atgcgcaata ctgtggtttg cgccacagga taaaaaaacc atccccaaac cttcctgcca
    20761 agaagaattg aaacaaaatc aattaagctt tttaaaatta agcttattat aagaggatat
    20821 gactttatat cgataaactt aaataaaaca tccctggctt ggatagcaac tctttatcaa
    20881 agcagaaaaa ttgttttaaa tcgagatatt ttgtcatggc agatttgaat cactggaaat
    20941 attttcagaa aaaactatag atgctcacac aaaatctcaa tctatttt
//
LOCUS       DBNCWK010000045        20340 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.045, whole genome shotgun sequence.
ACCESSION   DBNCWK010000045 DBNCWK010000000
VERSION     DBNCWK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20340
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.045"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(122..1489)
                     /gene="mpl"
                     /locus_tag="AC06IO_001453"
     CDS             complement(122..1489)
                     /gene="mpl"
                     /locus_tag="AC06IO_001453"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIO9874648.1"
                     /translation="MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLL
                     AAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGPQWLAENIL
                     SKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIE
                     ADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGV
                     AIKPRDDKALNEVLSRGQFSRIETLALSGEAEWVAELIDDSGSQFKILHNGGVVAEVS
                     WPLIGQFNVENGLAAIAATWNAGVNPMVAAKALEQFSPVKRRLEVRSNRHGITVYDDF
                     AHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAAEMAHALAPVDGAYVLEP
                     QDFNLKDFVSSWTCPYKICKNNEEIVNEIIKAVREGDAVLVMSNRGFNGIHQQLIDSI
                     DKLFM"
     gene            complement(1491..2039)
                     /locus_tag="AC06IO_001454"
     CDS             complement(1491..2039)
                     /locus_tag="AC06IO_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874649.1"
                     /translation="MNTLKLLFSYYWRLSLLKETPENSPYSVFLLIVMSVLFSIIMLI
                     QWNIAELDFSRDWLLSLMMATSLILSFIFYTYVILKFQNLASRLVQTSTCLLSAYIII
                     HVLATPLFLIDTYLSAENLKTPIFLFIGMIYLFITLGLSIWQFVITAHIYKFALGTTP
                     IKSVLAAFGLVAVNILTISFWR"
     gene            complement(2163..5984)
                     /locus_tag="AC06IO_001455"
     CDS             complement(2163..5984)
                     /locus_tag="AC06IO_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HIO9874650.1"
                     /translation="MTDNNLLANKFFKKIWMVLAVLIIVAAVFSSVFRALTPWAKQYK
                     GEVEQHLTSLLGQPVTVQTMETGWYWFEPVVKLKHIRIHDGHNKSIYLDRLIVGVNLF
                     KSLWNWRIQPGVLYIDDMHLVFREKDGHWCIEGISRNAMKDTDITPEKTREILDWLAQ
                     QEKLIVRHVSAHLHFNDGSLIPVNELNLSIVNKGGNYKIKGEARLEQTNATVFQLLGN
                     VQFDPSQIKNTNGRFYFSAINVLPAQWQNLLPKSSMQLEGGKGNVSLWVDLEKGNISS
                     IQAELRLKRLAWKLLNKKNSKLIQSFYANMGWKLDKQGWQFQADHMKLRVAGVTWPEN
                     QLSIRFNKNQNTYQIFTKSLIVESLLSEAINWPPAIQNVLQLKPHGILSDVQVLIKEN
                     QINYILTRFDQLGWRAKDNIPGVSNLSGVLNWQPTEGRLELDSENSTFDIKGYPAQKL
                     TIFNGAFDWKELNDGLRLSIERLVASQPELTISLEGAIDQLTQNSVGNIRLVGNFSGK
                     NLQKWMPYLPKKHMKAKLFAWLNNDIKRLGQATGKITINGMAKDFPFDNGNGEFSIVS
                     HVVGGDILITPEWRLIKDLEGYIKLKNRNLDIDIIDGDAQGTLLKQMNLRIDDIGKDR
                     ETLLIHGIVHGKAQQMLNFVMNSPLKKKLSALKILLIKGMLSLNLRLEIPLYPENDDN
                     LARGELTFENNIINIKHQMGDFSVRDVTGNLLFDEKGIQDSALTARAFGYPLNIKIQS
                     EKSPQPLTAISIEGECTVESLKKKWHLPIFSVLKGNILAKALLKITDDPNDLDNMKLS
                     SDLIGLAVDLPVPLGKTYKEKTPLQVNLDFNPQKTLRLRVNYNGRLSTDLLMQKQKGV
                     FKLKSGQIRLGSAQAVNQDHPGLGIAGTLDGFDLQAWKEVINRFSKENVNPSLMSLVN
                     IIDVKMSKLNFLNQQFDNMSLYAKKRPDNDWSVNLKQKKVDADLTYHSATNSLSGFVK
                     YLHLSEIGSSKNNKDTPSHATPGQIPNLNLRVDNFSIGKIQIGNITLKSHSTSEQWKI
                     DYCRIDAPYYQANFEGEWLQKGKFNQTKLQSKMYLKDLAKSLDRWAINPAVDAGKGYI
                     EFHGGWQGSLYDFSLSKLNGSMYLQLKNGRITHLSPETEEKLGLGKLLSILSLQTIPR
                     RLKLDFSDLSNDGYSFDIFKGNFNVNNGIMSTNNSYIDGPVAYASMKGDLDLVRRLYD
                     LDLNISPHITASLPVVATIAGGPIAGLAAWIANKIINQSMQKIAGYSYKISGPWDQPI
                     VQQLNIVKKLP"
     gene            complement(5974..6429)
                     /gene="fliJ"
                     /locus_tag="AC06IO_001456"
     CDS             complement(5974..6429)
                     /gene="fliJ"
                     /locus_tag="AC06IO_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HIO9874651.1"
                     /translation="MSDRLDRLIQLLKIKQEATQQAYIELVKAREQFNQNKARHEQLV
                     GYRQDYLQQLEVLGQQGSYVGPLRNRINFINHLDTALVQLNSYLSQLAKNRMKADLNY
                     KQAKTSEEGISKLIERVKRAELKQLQRIEQKETDEYAQKQWYSSLKHDR"
     gene            complement(6426..7775)
                     /gene="fliI"
                     /locus_tag="AC06IO_001457"
     CDS             complement(6426..7775)
                     /gene="fliI"
                     /locus_tag="AC06IO_001457"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HIO9874652.1"
                     /translation="MAIYQTQLHKILSGTRNKHHSGLLHCGRISRAIGLTIEAKGFNQ
                     PVGARCLVKINDEHSVQAEVVGFSNESVYLMSIGDIQGIAPGMKIIPTGRIAQVSVGH
                     QLLGRILNGSGMFIDDGPPGEMVETYPLIGQVINPLKRAPIEVPLDVGIRAINGLLTV
                     GRGQRIGLFAGSGVGKSVLLGMMTRFTEADVVVVGLIGERGREVKEFIECNLGLEGLR
                     RAVVVAAPADESPLMRLHGAKVATSIAEYFRDQGKHVLLLIDSLTRFAQAQREIALSI
                     GEAPATKGYPPSVFAKLPKLVERAGNGTSGGGSITAFYTVLTEGDDLQDPIADAARAI
                     LDGHVVLSRALAEQGQYPAIDLEASISRVMQGVVSEQHMKDMLLFKKYLSVYEKNKDL
                     ILLGAYAKGSDPLVDKAILAVNKLKDYLAQGMDEQVSYDESLSSLTKLISTLVGNDQ"
     gene            complement(7778..8416)
                     /locus_tag="AC06IO_001458"
     CDS             complement(7778..8416)
                     /locus_tag="AC06IO_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HIO9874653.1"
                     /translation="MSNEFEPYNKEIKKIEFTTWKYQASKDAEHSPSDEEELINELEV
                     VKQEAFQKGYADGFQQAQEEINAIKAELSKWLDLIQKPVQILDEQLTQEIIQTIIWLS
                     QHCVAVELSVNPDKLNDLLNKIKGELPSLTGKKSISMHPDDVRWIKEVFSESNIPGLH
                     ELLVADPALNRGDFYLQGEHSELDGRIQTRFTTLFNEYINKENIIPMGVSQE"
     gene            complement(8558..9547)
                     /gene="fliG"
                     /locus_tag="AC06IO_001459"
     CDS             complement(8558..9547)
                     /gene="fliG"
                     /locus_tag="AC06IO_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HIO9874654.1"
                     /translation="MDGIERAAILLLSMGEKNASEVLKHLEPRQVQKVGMAMSTMNSV
                     SKAKMQNVLIDFISAIEDQTSLTVDTEHYLRTVLIEALGEEKAIPFIDRILVSNKDSG
                     LNKLKWLDGRLIAEVIRNEHPQIIATILIHLDSEQSAEVVSYFSAEKRAEVLLRMCNI
                     DTVKPEAISELGHVIEKQLSGQTTGKSSSVGGIKTVADVINFLDGAMEEEVLERIKDW
                     DEELSEKIRDKMFVFENIKDMDDRSVQTLLREVTSDQLKLALKGTTETTKEKIYSNMS
                     KRAADLLRDDIEVQGPVRVSDVERAQRDILAIARNLAEDGKISLGAKGGDELI"
     gene            complement(9550..11184)
                     /gene="fliF"
                     /locus_tag="AC06IO_001460"
     CDS             complement(9550..11184)
                     /gene="fliF"
                     /locus_tag="AC06IO_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HIO9874655.1"
                     /translation="MALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSR
                     DPSYVPLYSQLNAQDSSEILTILERNSIDFKIDKNNGLLMVPADELQSARLKLAAQGL
                     PRDTAIGHDLLGSSGGVFNTSQFMENARYRQALEAELARTISKFNNIKSARVHLAIPR
                     ESAFVRDSQEPSASVFIDVYSGFEIKKHTIAAIVNLVASSIPNLSAGRVTVVDQDGQL
                     LNEGSGYNLFSETERFLDYRQNLEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYE
                     QTQELFNPELSALRSEQTMQESRNASNEASGVPGTLSNTPQPNSSLGQKNNPQQNATP
                     NNLPQQTSSDQMQAKDVRMQTTKNFELDKTISHTKNQPGTIKRLSVAVLVDNRPVMND
                     KTKKLEKKPLTKEEIEQIKLLVSDAIGLNVKRGDSLNVINSSFVKPDPIEAIPEEHFW
                     QKDSFWSVIKQIGGALFILAILFGVLRPMLKTLASNKDMDQATDNQDTGDFTLDGKIS
                     FRDVQDYETQLSLLRQVVDKEPKRVAQVVKSWVDRG"
     gene            complement(11356..11670)
                     /gene="fliE"
                     /locus_tag="AC06IO_001461"
     CDS             complement(11356..11670)
                     /gene="fliE"
                     /locus_tag="AC06IO_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HIO9874656.1"
                     /translation="MSDINTVNLINQMKTLAAQAEGIKPDSGLNHASFSSVFQQALNQ
                     VNELSQTADSLKTRFEIGDPNVSLGEVMIASQKSNLGFEATLRVRNKLVQAYQDIMNM
                     PV"
     gene            complement(11685..12998)
                     /gene="fleR"
                     /locus_tag="AC06IO_001462"
     CDS             complement(11685..12998)
                     /gene="fleR"
                     /locus_tag="AC06IO_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor FleR"
                     /protein_id="HIO9874657.1"
                     /translation="MSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELH
                     NPSVVLTDIRMDKLDGNQLLNEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQK
                     PFSAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALRVAQSDVGVMISGESGT
                     GKEVLAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQ
                     AQGGTLLLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEV
                     QAGRFREDLYYRLNVFPLQWHPLRERTSDIIPLANYLIRKHCQHKQPIVPVISPDAQK
                     AMLSYSWPGNARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEEKTKGTSKNLQI
                     HEYELIEKTLLENEGNRQKVAALLGVSERTLRYKLAKMREEGYVV"
     gene            complement(13022..14053)
                     /locus_tag="AC06IO_001463"
     CDS             complement(13022..14053)
                     /locus_tag="AC06IO_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIO9874658.1"
                     /translation="MNNMHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVW
                     LNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEVSLADGRRVHVALSSLESLPGIL
                     ITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDH
                     RIKNWIHRLQECHASIEQQIQDLLLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYA
                     AVLHVNNHLIARESSIHGESLIGAILNLIINALQSEATQIDLTLASMDDSGIQISVED
                     NGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVTLESTEGIGTQVTIY
                     LPCLNRNVG"
     gene            complement(14352..15656)
                     /locus_tag="AC06IO_001464"
     CDS             complement(14352..15656)
                     /locus_tag="AC06IO_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIO9874659.1"
                     /translation="MSLFNKEPDPPLAESLRPQHIDEVIGQSHLLGEGKPLRLCFMGS
                     KLHSMILWGPPGVGKTTIARLTAQAFDCEWIALSAVFSGVKDIRAAVEKAQEYLIHDK
                     QTILFIDEIHRFNKAQQDALLPYTESGLITFIGATTENPSFEVNSALLSRAQVYVLKP
                     LSEQELKLLFLRAHQRALSSLQFTEEAVDFLISCADGDARRLLNLLEQTKTACLTIKT
                     AVVDVELLHNVIVQNSRRFDKGGEAFYDQISALHKSVRGSNPDAALYWLCRMLDGGVD
                     PYYLARRIIRMSWEDIGLADPKAIQIANDAAATYERLGSPEGELALAQAVIYLSVAAK
                     SNAGYVAFNQAMEFIKRDKSREVPIHLRNAPTQLMKKLGYGHEYRYAHDEPHGYAAGE
                     CYLPEGMPEVNWYNPVQRGLETKIAEKLAFLRQLDKDVKKKI"
     gene            complement(15672..16280)
                     /gene="lolA"
                     /locus_tag="AC06IO_001465"
     CDS             complement(15672..16280)
                     /gene="lolA"
                     /locus_tag="AC06IO_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HIO9874660.1"
                     /translation="MNKLFLILLLIFSHEVFSQTSAEVLQSKLNAIQTMTANFSQIVK
                     AKNREVSRSSGSMALQRPGRFRWQTKDPLEQLIVADGQKMWIYDVDLEQVTVKNQEKG
                     LGGTAALFLSGYDETLTHDFDVSEKQKGKLTVFDLKSKSAKENFQRIKLIFSQSTLIG
                     LELYDQLGQITDVKLVQIKSNPKLPAKLFQFKPPKGVDVVKQ"
     gene            complement(16283..18667)
                     /locus_tag="AC06IO_001466"
     CDS             complement(16283..18667)
                     /locus_tag="AC06IO_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIO9874661.1"
                     /translation="MAKQHSGKQAGIPKKSMPSFIIKRLCEGSFILILTGALFVLLSL
                     FTYSTNDPGWSHASRSGMSVSNSGGQVGAYIADALYFAFGYFAFLLPVAFVYIAWAIL
                     KDFRSFKALDRMVLLLRTVGFILMVSGGCGLLSINHRFEAVDTIHSSGGIIGQTVGNG
                     WYQMLNMEGATLLLLAMFLVGTTWLTGLSWIKAIELIGCYTLNLFTFLDKFIRKGMQI
                     ISENFNKEKLKTPLIKTEQLPKPDNEKKKSVPKLFQDKKDKEQEKATPVLIASEEKPE
                     IVKPTNEFKEIRPPKTITPGALPSLSLLDKGQPGKPMGGYTHEELESLSRDVEQHLLD
                     FGIQADVVAVHPGPVVTRFELQLAAGVKVSKLTALAKDLARSLSVISVRVVEVIPGKT
                     VVGLELPNHSRQVVRLSDVLSADVYQQAHSPLSLALGVDIGGHPMVVDLAKMPHLLVA
                     GTTGSGKSVGINAMILSILFKASPEQVRLIMVDPKMLELSVYDGIPHLLTPVVTDMKE
                     AASALRWCVEEMERRYRLMAALGVRNLAGYNTKITEAAANGQPLLNPLWKPVDSMDET
                     APELQALPYVVVVIDELADMMMVVGKKVEQLIARIAQKARAAGIHMILATQRPSVDVL
                     TGLIKSNIPTRISFQVSSKIDSRTILDQQGAEQLLGHGDMLYLAPGSGAPLRVHGAFV
                     DDKEVHRIADDWRSRGEPDYVDDILKMGNENGDGAFDDDSGGQSVEDDDPLYDQAVEF
                     VIQTRKASISAVQRRLKIGYNRAARMIEEMERTGIVGPLDGGYRDVLVTSVTED"
     gene            18740..19690
                     /gene="trxB"
                     /locus_tag="AC06IO_001467"
     CDS             18740..19690
                     /gene="trxB"
                     /locus_tag="AC06IO_001467"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIO9874662.1"
                     /translation="MSISNHHRLIILGSGPAGYTAAVYAARANLNPVLITGMQPGGQL
                     TTTTDVDNWPGDIEGLQGPALMERMQKHAERFDTQVIFDHIVKADLAQAPFTLQGDST
                     TYTCDALIIATGASARYLGLPSETAYQGRGVSACATCDGFFYRNKSVCVIGGGNTAVE
                     EALYLSNLASSVTLIHRRDSLRAEKILQDKLFEKAHSGNIKIIWNSTLDEVLGDGKKV
                     TGAMIRNLKTGETDQLAMDGIFIAIGHTPNTAIFQDQLAMRDGYILIKSGLDGMATST
                     SVPGVFACGDVADHVYRQAITSAGFGCMAALDAEKYLDSI"
     gene            19693..>20340
                     /gene="aat"
                     /locus_tag="AC06IO_001468"
     CDS             19693..>20340
                     /gene="aat"
                     /locus_tag="AC06IO_001468"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIO9874663.1"
                     /translation="MAYDSNYTFPDPETSDKQGLLAIGGVLTPKRVLQAYSQGIFPWY
                     EPGNPVLWWSPNPRLILIPNEFKISRSLKKTLKKPFKLTVDTAFQRVISYCATCSDRS
                     NKTWITSEMIETYTQLHEMGYAHSFEIWDGSELVGGLYGISLGHAFFGESMFHTITDA
                     SKVALHFLCRIMQSWNFDFIDCQLPTLHLMSLGAKIISRKEFLHMLQETLKYPDKK"
BASE COUNT         5991 a         4453 c         3413 g         6483 t
ORIGIN      
        1 ccttttactg tgatgatctt tttatgttgg ggctttgcaa acaataggac ttgactctta
       61 ttttacctgt ttctcaatat catgatatgt tctcaagaca tttcaaaata aaaatgagtc
      121 gttacataaa aagtttatct atagaatcga tcaattgttg atgaattcca ttaaatcctc
      181 tattactcat taccaataca gcatcgccct ctcttactgc tttgataatt tcgttaacta
      241 tctcttcatt gtttttgcaa attttatacg gacaagtcca ggagctaaca aaatctttta
      301 aattaaaatc ctgaggctcc aggacatagg caccatcaac aggagctaaa gcatgagcca
      361 tttctgcggc atgtacaccc gttctcatag tatatgaagc aaactccata acagcaaaaa
      421 tacgttgatg tcgtccactt ctttttaaag catcaatagt tttagtaatt gcggttggat
      481 ggtgggcaaa atcatcataa acggttattc catgtcgatt ggaacgaact tcaagccttc
      541 gtttcactgg agaaaactgt tctaaagcct tggctgcaac cataggattt actccagcat
      601 tccaggtagc agcaattgca gccaaaccat tttctacatt gaattgccct atcaacggcc
      661 aagatacttc agcaaccact cccccattat ggagtatttt aaattgtgaa ccactatcgt
      721 caatcagttc ggcaacccat tcggcttctc cagatagtgc cagtgtttca atacgcgaaa
      781 attgacctcg tgatagaact tcattcagag ccttatcatc tcgaggtttg atagccacac
      841 cacttttggg aattgttctt aataaataat gaaattgttg ttgaatagct gccaagtccg
      901 gataaatatc ggcatgatca aattctaaat tgtttaatac tgcaatattt ggtcgataat
      961 gcataaattt aggtctttta tcaaaaaaag cactatcgta ctcatctgcc tctatgacaa
     1021 accatctccc tttgccaagg caggcactgg tattgaaatc actagagacg cccccaatga
     1081 gaaatcccgg attcattcca gcttgatcca aaatccaggc aatcattgaa gtggtagtcg
     1141 ttttgccatg agttccagct accgcaatca cctgatattt tgataaaata ttttcggcaa
     1201 gccattgagg gcctgacata tatggttttc ccgactccat aactgcttca agaaccggca
     1261 ttccccgctt aatagcatta ccgacaataa ccatgtcaga ttgcaatgct aaagtggaat
     1321 catcgtagcc ttcagtccat gaaatacctt tggcagcaag caaatcactg ataggcggat
     1381 agcaattggc atcgctgcct gtcaccctat atcctgcgtc gcgggcgagc aaagccaggg
     1441 cactcataaa ggttccactt actcctagta tatgcaaatg tgtcatcatg tcatcgccaa
     1501 aaagaaatag ttaaaatgtt aacagcgact aaaccaaaag cagctaaaac tgattttatc
     1561 ggtgtagtgc ctaaagcaaa tttataaata tgggctgtga ttacaaattg ccaaatagaa
     1621 agccccaaag taatgaacaa atatatcata cctataaata aaaaaatggg ggttttaaga
     1681 ttctccgcag acaaataagt gtcaattaag aacaatggtg tcgccaaaac atgtataata
     1741 atataggcac tcaataaaca tgttgatgtt tgtactagcc tggacgcaag attttgaaac
     1801 tttaaaataa cataggtata gaatataaaa gacaaaatca ggctcgttgc catcattaaa
     1861 cttaataacc aatcgcgaga aaaatcaagt tcggcaatat tccattgaat cagcattata
     1921 atagagaaca gaacactcat tactataaga agaaaaacgg aataaggaga attctccggg
     1981 gtttccttca ataggctaag acgccagtaa taagaaaata gtaattttaa tgtattcata
     2041 attatttttg attattttct tgaggcttaa gtactctctg aacaaggcag aatattttat
     2101 attcctccat aagccctaag tgacttcata actgtttact aaatagtctt gtcttccttt
     2161 agttaaggca atttcttcac tatattaagc tgttgcacaa taggctggtc ccaagggcca
     2221 gaaattttat aactataccc cgcaattttt tgcatgcttt gattaataat tttatttgca
     2281 atccatgcag caagaccagc aatcggacct cctgctatcg tagctacaac gggtaaactg
     2341 gcagtgatat gcggtgaaat gtttaaatcc aaatcatata atcgtcttac caaatccaga
     2401 tctcctttca tactagcata agctactgga ccatcaatgt aactgttatt ggtgctcata
     2461 atgccattat tgacattaaa gttgccttta aatatatcaa aactatagcc atcatttgag
     2521 agatcactaa aatcaagctt taaccgtctt ggaatagttt gcaaactcaa tatgcttaat
     2581 aacttcccta aaccaagttt ctcttcagtc tccgggctca aatgagtaat gcggccattt
     2641 ttcagctgta aatacatgga gccattcaac ttggacagag aaaaatcata taaggaacct
     2701 tgccaacccc catgaaattc tatatatccc ttccctgcat ccaccgctgg attaatcgcc
     2761 caacgatcaa gactcttggc caaatctttc aaatacattt tagactgtag ctttgtttga
     2821 ttaaacttcc ctttctgaag ccattcccct tcaaaattag cttgataata aggtgcgtct
     2881 atcctgcaat aatcaatctt ccactgttct gatgtagaat gacttttaag ggtgatattc
     2941 ccaatttgaa tttttccaat tgaaaaatta tcaactctta aattcaaatt ggggatttgt
     3001 ccaggagtag catgtgaagg agtatcttta ttattttttg aggatccgat ttcagataaa
     3061 tgtaagtatt tgacaaagcc actcagagaa ttggttgccg aatggtaggt taaatccgca
     3121 tcgacttttt tctgttttaa attaacagac caatcattat cagggcgttt tttcgcatac
     3181 aaagacatgt tatcaaattg ctgattcaaa aagtttagtt tactcatttt tacatcaata
     3241 atattaacca aactcatgag tgaggggttc acattctctt tagagaatct gtttattacc
     3301 tctttccatg cctgcaaatc aaagccgtct agagtaccag ctataccgag tcccggatgg
     3361 tcctggttta cagcttgcgc actacctaaa cgtatttgac ctgattttaa cttaaacacg
     3421 cctttttgct tttgcatcaa tagatcagtg cttaatcgtc cattgtaatt aactcttaaa
     3481 cgaagagtct tttgtggatt aaaatccagg ttaacctgta agggggtttt ttctttatat
     3541 gtttttccta aaggtactgg aagatcaact gccaatccaa tcaaatcgga actcaacttc
     3601 atattatcca aatcattagg atcgtcagta atttttaata atgccttagc taaaatattc
     3661 cctttgagta cagaaaaaat aggcaagtgc cattttttct ttagggattc tacagtgcat
     3721 tcaccctcga ttgatatcgc ggttaatggt tgtggagatt tttccgattg aattttaata
     3781 ttcaggggat agccgaatgc cctggcagtt aaggcactat cctggatgcc tttttcatca
     3841 aacagtaaat ttccagtaac atcccttaca gaaaaatctc ccatctgatg cttgatgttg
     3901 attatattat tttcaaaggt taattcacct cgggccagat tatcatcatt ctctggataa
     3961 agaggtattt ccaaacgcaa attgagtgat agcatcccct ttattaataa tatttttaaa
     4021 gcagataatt ttttctttaa tggggaattc ataacaaaat tgagcatttg ttgtgctttc
     4081 ccatgaacaa ttccatgaat taataaggtt tccctatctt tgccaatatc atctatacgc
     4141 aaattcattt gtttaagcag agtcccttga gcatcaccat caatgatatc aatgtcaaga
     4201 tttctatttt ttagtttaat ataaccttcc aaatctttaa tcaatcgcca ttctggagta
     4261 attaaaatat ctccaccgac aacatgactt actatagaaa actccccatt gccattatca
     4321 aatgggaaat ccttcgccat tccattaata gttatcttgc ctgttgcttg ccctaaacgt
     4381 ttgatgtcat tatttaacca agcaaataac ttggctttca tgtgtttttt aggcaaataa
     4441 ggcatccact tctgtaaatt tttcccagaa aaattaccca ctaaacgaat attgcctaca
     4501 gaattttggg tcagctgatc aatagcgcct tccagactaa tagtcaattc gggttgactt
     4561 gcaaccaagc gttcaatact caaccgcaac ccatcattta actctttcca atcaaaagca
     4621 ccattaaaaa tggtcagttt ttgagcggga taacctttga tatcgaaagt tgaattctct
     4681 gaatctaact caaggcgtcc ttccgtaggc tgccaattta aaacgccgga taaattactc
     4741 actccaggaa tattatcctt tgctctccat cctaattgat caaatcgagt taaaatataa
     4801 ttaatttgat tttctttgat taatacctga acatcactca atattccatg cggttttaac
     4861 tgcagtacat tttgaatagc cggtggccaa ttaattgctt cggataatag tgattctaca
     4921 ataagggatt ttgtaaagat ttgataggta ttctgatttt tattaaaacg aattgacaat
     4981 tggttctctg gccaagtgac ccctgctact cttagtttca tatggtctgc ctggaactgc
     5041 cacccttgtt tatccaattt ccagcccatg ttggcataaa atgattggat caacttacta
     5101 ttttttttat ttaataattt ccatgccaga cgcttaagtc tcaattctgc ctgaattgat
     5161 gaaatattcc ctttctccaa atcaacccat aatgaaacgt ttcctttacc tccctccagt
     5221 tgcattgagg attttggcaa taaattttgc cattgtgcag gtaaaacatt aatagctgaa
     5281 aaatagaagc gtccattggt gttcttaatt tgagaaggat cgaattgtac attccctagc
     5341 agttggaaaa cggttgcatt tgtttgttcg agtcttgctt ccccctttat cttataattt
     5401 ccccccttat taactatcga caggtttaat tcgttaacag ggattaatga cccgtcgtta
     5461 aaatgtaagt gagcggaaac atgccgtaca attaattttt cctgctgagc gagccaatct
     5521 aatatttctc tagttttttc aggtgtaata tcagtgtctt tcattgcatt acgtgaaatt
     5581 ccctcaatac accaatgccc gtctttttca cgaaaaacca aatgcatatc atcaatataa
     5641 agtacgccag gttgaatgcg ccaattccat agggatttaa ataaattaac accgacaatt
     5701 aacctatcca gataaattga cttattatga ccatcatgaa ttcttatatg ttttaatttg
     5761 actactggtt caaaccagta ccaccctgtt tccatagttt gaacagtaac aggttgaccc
     5821 aaaagtgaag tcaaatgctg ttccacctcc cctttatatt gtttagccca gggagtaaga
     5881 gcgcgaaata cactggaaaa gacagctgca acaattataa ggacggctaa taccatccaa
     5941 atttttttaa aaaatttatt tgccaaaagg ttattatcgg tcatgtttta aactactata
     6001 ccattgcttt tgtgcatatt catcagtttc cttttgctca atacgctgta attgcttcag
     6061 ttcagctctt tttacgcgtt caattaactt acttattcct tcttctgaag tttttgcttg
     6121 cttataattt aaatccgcct tcatcctgtt ttttgcaagt tgactcaagt aggagtttaa
     6181 ttgtaccaaa gcagtatcca aatgattaat aaaattaatt cggttgcgta aaggccctac
     6241 gtaacttcct tgttgcccca atacttccag ttgctgtaaa taatcttgtc tgtaaccaac
     6301 taattgttca tgccttgctt tattttgatt aaattgctct cttgctttca ccaactctat
     6361 ataggcctgt tgagtcgctt cctgtttaat ttttaataac tgaatcaaac gatctaatct
     6421 gtcactcatt ggtcattccc taccaaagtt gaaatcagtt tggttagcga tgataaactc
     6481 tcatcgtagc ttacttgttc atccattcct tgcgccaggt aatctttgag tttattcact
     6541 gctaatatcg ctttatccac caggggatcg cttccttttg cataggctcc taataaaatt
     6601 aaatccttgt ttttttcata aacagacagg tattttttaa acaataacat atctttcata
     6661 tgttgttcag agacaacacc ctgcataact cggctaatag aagcttcaag atctattgca
     6721 ggatactgcc cttgctctgc aagcgctctg cttaatacaa catgaccatc caaaatagct
     6781 cgtgccgcat ctgctatggg atcttgcaaa tcatctccct cagtcaagac agtataaaat
     6841 gccgtaattg aaccaccccc cgaagtccca tttccagctc tttcaaccaa tttaggcaat
     6901 ttggcaaata ccgatggcgg atatcctttt gtagcaggag cctcaccaat cgataacgcg
     6961 atttctcttt gtgcctgggc aaaacgggtt aaagaatcaa tcagcaagag aacatgttta
     7021 ccttgatccc gaaaatactc agcaatgctt gttgcaacct tcgcgccgtg caaacgcatc
     7081 aaagggcttt catccgctgg agcggcaact acaaccgctc gcctcaaacc ttccagaccc
     7141 aaattgcact caataaattc ttttacttcg cgcccacgtt ctccaattaa tccaaccaca
     7201 acaacatcag cttcagtaaa acgcgtcatc atcccaagca aaactgattt gccaactcca
     7261 cttccagcga acaatcctat ccgttgtccg cggccaacag ttaataagcc attaatagca
     7321 cgaatcccaa catccaatgg aacttcaata ggggctcttt ttaaaggatt gatcacttgc
     7381 cctatcaatg gataagtctc aaccatttca ccaggaggtc catcgtctat aaacattccc
     7441 gaaccattta atattcttcc taacaattga tggccaacac ttacttgagc aatgcgtccg
     7501 gtaggaataa ttttcattcc aggggctata ccttgtatat caccaataga cattaaataa
     7561 acactttcat tactaaaacc aacaacttct gcctgaacag aatgttcatc attgatttta
     7621 accaggcaac gcgctcctac aggctgatta aatccctttg cttctatggt taaacctatt
     7681 gctctgctga ttctaccaca atgtaacagt ccggaatgat gtttattcct tgttcctgac
     7741 aaaattttat ggagttgtgt ttggtaaatg gccatcacta ttcctgagaa acgcccattg
     7801 ggattatatt ctctttatta atatattcat tgaataaggt agtaaaacga gtttgaattc
     7861 gaccatctaa ctcgctgtgt tcaccttgta aataaaaatc tcctcgattt aatgctggat
     7921 cagccaccaa taactcatga agaccgggga tattactttc agaaaatact tctttaatcc
     7981 atcgcacatc atcaggatgc atgcttatag acttcttgcc agttaaagaa ggtaactcac
     8041 cttttatttt atttaataaa tcatttagct tatctggatt aacagaaagc tcaacagcaa
     8101 cacagtgttg acttaaccaa atgatggttt gaatgatttc ctgcgtcaat tgctcatcta
     8161 aaatttgaac gggcttttga attaaatcca gccatttgga gagctccgct ttaattgcat
     8221 taatttcttc ttgtgcttgt tgaaatccat ctgcataacc tttttgaaaa gcttcctgct
     8281 tcactacctc caattcatta atcaactctt cttcatcaga cggcgaatgt tcagcatctt
     8341 tggaagcctg atacttccaa gtggtaaatt caattttttt tatctcttta ttatacggct
     8401 caaattcatt tgacatagtt atctaacctt atcccgggcc caaatcatac caaaaattac
     8461 cacttaggac aaataaaata caattttgat acggactctt gcttaatgat ttttactatt
     8521 ttcataaaat gtctaaaact catcctggga aaagtaacta gattaattcg tctccgccct
     8581 ttgctcccag gctaatcttg ccatcttcag ccagatttct tgctatggct aaaatatctc
     8641 gctgggcacg ctccacatca ctgactctta caggtccttg cacctcgata tcatctctga
     8701 ggagatcagc tgctcgctta gacatattcg aatagatttt ttctttcgtt gtttcagtag
     8761 tacccttcaa tgctaatttt aattgatcag atgtcacctc tcgcaataaa gtttgtacac
     8821 ttctatcatc catgtctttg atgttttcaa aaacaaacat cttatctctt atcttttcgc
     8881 ttaactcctc atcccaatcc ttaattctct ccagtacctc ttcttccata gcgccatcca
     8941 aaaaattaat cacatctgct acggttttaa ttccgccgac agacgatgat ttacctgtgg
     9001 tttggccact aagctgcttt tcaatcacat gtccaagttc agaaattgct tcaggtttta
     9061 ctgtatcaat attacacatt cgtaataaca cttctgctct tttttctgcg ctaaagtaac
     9121 taaccacttc agcggactgc tcactgtcca gatgaattaa tatagtggca atgatttggg
     9181 gatgttcatt acgaatcact tcagctatca gacgtccatc caaccatttc agcttattca
     9241 aaccactgtc tttatttgat accaatattc tatcaataaa tggaattgcc ttttcttcac
     9301 ccaatgcctc aatgagaacg gttctgaggt aatgctctgt atcaacggtt aagctggttt
     9361 gatcttcgat tgcgctgata aagtcaatca gaacattctg cattttcgcc ttgctgacgc
     9421 tattcatcgt tgacatagcc attccaactt tttgtacttg cctgggctct aaatgcttta
     9481 atacttctga agcatttttt tctcccatgc ttaacaataa aatagctgct cgctctattc
     9541 cgtccataat tacccccgat caacccatga tttcactact tgtgcgacgc gtttgggctc
     9601 tttatcaacc acttgacgaa gcaaactcag ctgagtttca taatcctgaa catctctgaa
     9661 tgatattttt ccatctaatg taaagtctcc tgtatcctga ttatcagtgg cttgatccat
     9721 gtctttatta cttgccaatg tttttaacat aggtcttaat acaccaaata ggattgccaa
     9781 tataaaaaga gccccgccaa tctgcttaat cacagaccaa aaactgtctt tttgccaaaa
     9841 atgttcttct ggaattgctt ctataggatc tggctttaca aaactgctgt tgatcacatt
     9901 caagctatct cccctcttaa cattcaatcc tatggcatca gaaaccaata actttatttg
     9961 ttcaatttct tctttagtta gcggtttctt ctccagcttc ttggttttat cattcattac
    10021 aggtcgatta tctaccaata ccgcaaccga caagcgtttt attgttcctg gctgattttt
    10081 agtatgactt attgttttgt ccaattcgaa atttttagtg gtttgcatgc gaacatcctt
    10141 tgcctgcatt tgatctgacg atgtttgttg aggtaagtta tttggcgttg cattttgttg
    10201 aggattattt ttttgcccaa gagaactatt tggttgagga gtatttgata atgtcccagg
    10261 aaccccgctt gcttcatttg acgcattacg actctcttgc atagtttgtt cgctgcgtaa
    10321 agcggagagc tccggattga acaattcttg agtttgttca tagctggtaa agtcaatatc
    10381 tgcagatact ttggccctga cacggccgta tcccaatatt ggggttaaga tatcctgaat
    10441 tttttgagcg tattgatgtt ccaggttttg tctgtaatca agaaaacgct cagtctccga
    10501 aaagaggtta tatccgctac cctcatttag caattgtccg tcttgatcaa ctactgttac
    10561 tcttcctgcg cttaaattcg gaatacttga tgctactaaa ttgacaattg ctgcaatggt
    10621 atgtttcttt atttcaaatc ctgaatacac atcaataaat acagaagcac taggttcttg
    10681 tgagtctctt acaaaggctg actctcttgg aattgccaag tgtactctgg ccgatttaat
    10741 attattaaat ttactgatcg tgcgtgctaa ttccgcctca agagcttgcc tatatcgggc
    10801 gttttccata aattggctgg tattaaacac cccaccactg ctgcctaaaa ggtcatgacc
    10861 tatggcagta tcgcgaggca atccttgtgc ggccaatttc aaacgcgcgc tttgcagctc
    10921 atcagcaggc accatcagca agccattatt cttgtcaatt ttgaaatcaa tactattgcg
    10981 ctctaaaata gtcaaaatct ctgatgagtc ctgtgcgttg agctgactat ataaaggaac
    11041 atagcttgga tcacgagacc atagaaccac tgctacgcct attgcaatac tggccgccaa
    11101 tccaaacaac actccaattt ggcgtggtat agataagctt gcaaattttg tagctgctac
    11161 tgaagcatta tcagccaatg ccatgattta ctcctgtaat ttttatcctt ttttcagaat
    11221 acttcactct gagctttaaa actttattga gaacatttgc aaactcatta tggcattcaa
    11281 aaaccccata aaaagctgtt attgagtttg atcttgttta tctgttgaaa aagttctact
    11341 acccaaatca tcgcactaaa ccggcatgtt cataatatcc tggtatgctt gtaccaattt
    11401 gtttctaact cttaatgtcg cctcaaaccc taaattggat ttttgacttg ctatcattac
    11461 ttcacccaag gacacattag gatcgcctat ttcaaagcgg gtctttaaac tatctgctgt
    11521 ttgtgataat tcattgactt gatttaatgc ctgttgaaaa accgaactga aagaagcgtg
    11581 atttaaacca gaatccggtt ttatcccctc agcctgggcg gccagagttt tcatttgatt
    11641 aattaaattt actgtattaa tatcgctcat tctctcagcc cctgttaaac cacataccct
    11701 tcttcacgca tttttgcgag tttatagcgc aaagtccttt cgctaacccc caataacgca
    11761 gcaacttttt gtctgtttcc ttcattttct aataatgttt tttctattaa ttcatactca
    11821 tgtatctgta aatttttact cgtacccttt gtcttctctt ccgaaggcat ggtagttgaa
    11881 ttcgtaatct gcaaatgctc ttcttcgatg ataccttgag tctgtaacac cagagctctt
    11941 tgtataacat tatccagctc tctcgcattc cctggccatg aataggacaa cattgccttt
    12001 tgcgcatccg ggctaatgac tggaactatt ggttgtttat gctgacaatg tttacggatt
    12061 aaataattcg ctaatggaat aatatcactt gttctctctc ttagtgggtg ccattgcaga
    12121 ggaaaaacat tcaatcgata gtaaaggtcc tctctaaaac gccctgcctg aacctcttcc
    12181 tttaattgcc tgtttgtcgt cgcaataatg cgcacatcaa ggtttattat tttgtttgaa
    12241 ccaatccttt ctacttcttt ttcctgtaaa actctaagta attttgcctg caaacctaag
    12301 gacatttcac ttacttcatc taaaagtaaa gttccgccct gggcttgctc aaacttgcca
    12361 ggggttgatt tgtatgctcc ggtaaaagca cctttttcat aaccaaacag ggttgcttcc
    12421 agcatttgtt caggaattgc agcacaatta attgcaataa aaggttgttt gcttcgataa
    12481 gaatgatcat ggataaaatg tgcgagcact tcctttccag taccgctttc tccactgatc
    12541 attactccca catcggattg tgcgactctc aaagccattg tcagtaaggc ttggctttta
    12601 ggatcccgtg caataggatc attatcctct tcactcagat cagtttttat atactgcttt
    12661 attttttcaa ttaagacttg cgcgctgaag ggcttttgca aataatcaac agcgccattg
    12721 cgaatcgctg taaccgcatc ctctacagaa ccataagctg tcattagaat aaccggcaaa
    12781 ccaggcctct ttttttgtat ttcatttaat aactgatttc catctaattt atccattctt
    12841 atgtcagtga gtacgacact tggattatgt aattcaagaa gtgctaatgc ctctttaccg
    12901 tctttagcag tgatataggt atggcctgag atcgtcatag tctcagccaa cgcttctctt
    12961 aatactgaat catcttcaac tattaaaaca tgactcatag aaaatccttt gtttgataaa
    13021 gttaccctac gtttctgttc aggcaaggta aataaatagt tacctgtgtc cctatccctt
    13081 cagtcgactc aagagtcact cgaccaccat gcgcttttac tacagcaaaa accactgcta
    13141 atccaaggcc ggtaccttgt gcttttgttg tatagaacgg cgaaaatgct tgtgccctga
    13201 cttcttcaga cattccgttt ccattatcct ctactgatat ttgtatacct gaatcatcca
    13261 tggatgcaag tgttaaatca atttgggttg cctcagattg cagcgcattg attatcagat
    13321 ttaaaattgc tccaatcaaa gactctccat gtatggagga ttcacgagca ataagatggt
    13381 tattaacatg taatacagct gcataagact cgatgtatgg ctgagctctt tgcattaatt
    13441 gagagcacca aaactccata tcgatatact ttggctctat ggaggttcct ctggcaaata
    13501 ataataaatc ctgaatttgt tgctctatac tggcatgaca ttcttgcaat ctgtgaatcc
    13561 agtttttaat gcgatgatcc aaacttggga gattatttaa atgctcggta tacaatatgg
    13621 cagaagacaa aggagttcta atctgatgag ctaattgtgc ggtcattgta ccaatagaaa
    13681 ccaatcgttt ttcattttct ttggcttctt cataatccct gcttgcagta atgtcagaca
    13741 aagtaatcaa tataccgggt aaactctcta aagacgaaag ggctacgtgc acacgccttc
    13801 catcagccag agacacttca tgcccatcat cagctcttgg aacaaatgct ttttgaatca
    13861 catctaccca tagtgaacca atcagtccat aaccaagcat cgcttcagct gaaggattta
    13921 accacaccac tttgccttgg gcattcaaaa ttaccaaggc ggtaggcaaa ctggacaaaa
    13981 tgtcccattc agataattct atctgtggtt tgactgcaca agtttgataa tttgaataat
    14041 gcatgttatt cattttaatt attattatga aatgtctcta gcaaagttca ggccaaaatt
    14101 aaaatgtatt tgaaatcaat taattaacca attgaaaacc agattttttt gaatgtattt
    14161 aaaaatataa acaatttgta atttttaaat cattgtaact tgggttaagg agatttcaaa
    14221 gaatcatcct tgcttcctac agtcagaatt ttgacatact ctttatactt caccggatac
    14281 caatgtcgat tggatttctt ctttgggata aattgggtga caaacctaac ctggcttaaa
    14341 cagagaacaa atcaaatttt cttttttaca tctttatcta attggcgcag aaaagcgagc
    14401 ttttctgcaa ttttagtttc taaacctctt tgtaccggat tataccaatt tacttctggc
    14461 attccttcag gtaaataaca ttcaccagcc gcatagccgt gtggttcatc atgagcatat
    14521 cgatattcat gtccataacc tagttttttc attaattgag taggagcgtt tctcaagtga
    14581 atgggtactt ctctggactt gtctcttttt ataaattcca tagcttgatt aaatgcgaca
    14641 tatcccgcat tgctcttggc agcaacagac aaataaatta cagcttgcgc caaagcaagc
    14701 tcgccctcgg gtgagccaag cctctcataa gttgcagcag catcattagc gatttgaatc
    14761 gctttcggat ctgctaaccc aatgtcttcc catgacattc taataatacg tctggctaaa
    14821 taatatggat ccacaccacc atccagcatt cgacatagcc aatataatgc cgcatcagga
    14881 ttagagcctc gtaccgattt atgtaaagca gaaatctgat cgtaaaacgc ttctccacct
    14941 ttatcaaaac gtcgactatt ttgtactatc acattatgca aaagttctac atcaacaact
    15001 gctgttttaa ttgttaggca ggctgttttt gtttgctcaa gtaaattgag taatctcctg
    15061 gcatccccat cagcacaaga aattaaaaaa tcaacggctt cctcggtaaa ttgcagagaa
    15121 gataaagctc tttggtgagc cctaagaaat aagagtttta actcctgctc cgataacggt
    15181 tttaaaacat aaacttgtgc ccgtgataat aacgctgaat taacctcaaa agacggattt
    15241 tctgtagttg ccccaatgaa agtaattaag cctgattctg tataaggcaa taaggcatct
    15301 tgctgagctt tattgaatct atgaatttca tcaataaata agatagtctg cttatcatga
    15361 attaaatatt cctgcgcttt ctcaaccgca gctcgaatat ccttaactcc tgaaaataca
    15421 gctgataatg ctatccattc acaatcaaaa gcctgtgcag ttaatcttgc tatagtagtc
    15481 ttaccgaccc caggtggccc ccataagatc atagaatgca atttggagcc cataaaacaa
    15541 agtcttagag gtttaccctc tccaagcaaa tgactttgac ctatgacctc atcgatatgc
    15601 tgaggcctta aactttcagc caatggtgga tcaggttctt tattgaatag gctcataaat
    15661 caacattaaa attattgttt taccacatcc acacctttag ggggcttgaa ttggaacaat
    15721 ttggcaggta acttaggatt tgacttaatc tgcactaatt taacatcagt aatttggcct
    15781 agctgatcat ataactccag accaatcaaa gtactttgac taaaaattaa cttaattctt
    15841 tgaaaatttt cttttgcaga tttggatttc aaatcaaaaa cggtcaattt ccctttttgt
    15901 ttttcactga catcgaaatc atgagtaaga gtttcatcat agccgctcaa aaacaatgca
    15961 gcagtaccac ctagtccctt ttcctgattt ttgactgtga cttgttcaag gtccacatca
    16021 taaatccaca ttttttggcc gtcagcaaca attaattgtt ccaatggatc ttttgtttgc
    16081 cagcgaaatc gtccaggacg ttgcaatgcc atggaacctg aagatcgtga cacttcacga
    16141 ttctttgcct ttacaatttg actaaaattt gcagtcatgg tttgtatagc atttaattta
    16201 ctctgcagta cttcggcaga agtttgacta aacacttcat gcgaaaaaat gagcaacaaa
    16261 atgagaaaca atttattcat attcagtcct cagtcactga tgttactaat acgtcacgat
    16321 aaccaccatc caatggaccg actattcctg tcctctccat ttcctctatc attctggcag
    16381 cgcgattata accgattttt aatcttctct gtaccgcaga aatactagcc ttgcgtgtct
    16441 gaattacaaa ttctaccgcc tgatcataaa gaggatcatc gtcttctacg gattgccccc
    16501 cactatcatc atcgaaagca ccatccccat tttcatttcc cattttaagg atgtcatcaa
    16561 cataatcagg ttcacctctt gaccgccagt catcagcgat acgatgaact tccttatcat
    16621 cgacaaaagc accatgcaca cgcaaaggag ctccacttcc tggtgccaga tataacatat
    16681 caccatggcc gagcaattgt tcggctcctt gttggtcaag tatggtgcgc gaatcaattt
    16741 ttgaagacac ctgaaatgat attcttgttg gaatattgga tttaattaaa cctgttaata
    16801 catccactga aggtctttgt gtcgcaagaa tcatatggat tcctgctgct cttgcttttt
    16861 gcgcaatacg agcaatcaat tgctccactt tcttgccaac aaccatcatc atgtcagcaa
    16921 gttcatcaat tacaacaact acataaggca gtgcttgcaa ttctggtgca gtttcatcca
    16981 tagaatcgac gggtttccat aatggattta acaatggctg accattagct gccgcttccg
    17041 taattttggt gttataccct gccaaattcc taacacccaa cgcagccatt aacctataac
    17101 gcctctccat ttcctctaca caccagcgca gtgcgctcgc tgcctccttc atgtcggtaa
    17161 caacaggagt cagcaaatgt ggaatcccat cataaactga taattccaac attttgggat
    17221 cgaccataat taaacgcact tgctctggac ttgctttaaa aagaatactt aaaatcatgg
    17281 cattaatacc aactgattta ccagaaccag ttgttcctgc aaccagtaga tgaggcattt
    17341 ttgccaaatc aaccaccata gggtgcccgc caatatcaac tcccaaggct aaagataagg
    17401 gagaatgagc ttgctgatat acgtcagcag ataaaacatc tgataacctg accacttggc
    17461 gtgaatgatt gggtaattca aggcccacaa cagttttacc aggaataacc tctacaactc
    17521 ggacagaaat aactgataac gaacgagcca aatcctttgc caaagcggtc agtttgctca
    17581 ctttgacccc tgctgccaat tgcaattcaa atcgggttac cactggcccc gggtgaacgg
    17641 ctacaacatc tgcctgaata ccaaaatcaa gcaagtgttg ttctacatct cttgaaagac
    17701 tttccaattc ctcatgtgta taacctccca tcggcttccc tggttggcct ttatctaaca
    17761 acgacagact gggaagagct ccaggggtta ttgttttagg aggacgaatt tccttgaatt
    17821 cattggtagg tttgacaatt tcaggttttt cttcacttgc aattaaaaca ggtgttgctt
    17881 tttcttgctc tttatctttc ttgtcctgga ataatttagg aacagatttc tttttttcat
    17941 tatcaggttt agggagttgt tcggtcttga tcaatggtgt ttttaatttc tctttgttaa
    18001 aattttcaga aataatttgc attccttttc tgatgaattt atcaagaaat gtgaaaagat
    18061 ttaatgtgta acatccgatt aactcaattg cctttatcca ggataatcca gtcagccagg
    18121 ttgttcccac aagaaacatt gctaataaaa gtaaagtggc gccttccatg ttaagcattt
    18181 ggtaccaacc atttccaact gtttgtccga taatacctcc tgaactgtga atggtatcta
    18241 ctgcttcaaa gcgatgattt atactcaata aaccacaacc accagaaacc atgaggataa
    18301 aaccaaccgt tctcagtaac agaaccattc tgtccaacgc tttaaaagag cgaaaatcct
    18361 tcaaaatggc ccaggcaatg taaacaaatg ctactggtaa taagaaagca aaatatccaa
    18421 atgcaaaata aagcgcatcg gcaatatacg caccaacttg tccccctgag tttgaaacgg
    18481 acatacctga acgggaggca tgagaccaac ctggatcatt ggttgaatag gtaaataaag
    18541 acaagagaac aaaaagagct cctgtcaaaa tcaatataaa actcccctca cacaagcgtt
    18601 ttattataaa gctcggcatg gattttttag ggattccagc ctgttttccc gaatgttgtt
    18661 ttgccatagg tattttaatg tttttatttt tgtcataata tttgccatct taacacaaaa
    18721 aataaggaag gcagagacaa tgagcataag caatcaccat cgcttaatca tactcggttc
    18781 aggtcctgca ggttacactg ctgcagtcta tgccgcaaga gccaatctga atcccgtttt
    18841 gatcaccgga atgcagcctg gcggacaatt aacaaccacg actgatgtag acaactggcc
    18901 cggagatatt gaaggattac aaggccccgc cctaatggag cgtatgcaaa aacacgcgga
    18961 acgctttgat actcaagtca tattcgatca tattgtcaaa gctgatttag cgcaagctcc
    19021 ctttacacta caaggggata gtactactta cacctgcgat gccttgatta ttgcaaccgg
    19081 agcctctgct cgttatttgg gattaccctc agaaacggcc taccaggggc gaggtgtctc
    19141 agcgtgtgct acttgcgatg gattttttta tcgcaataaa agtgtttgcg ttattggagg
    19201 aggcaatact gccgttgaag aagcacttta tttatcaaat ttggcatctt ccgttactct
    19261 gattcacaga cgagacagct tgcgtgcaga aaagatactg caggataaac tctttgagaa
    19321 agcgcattcc ggtaacatta aaataatatg gaactcaacc cttgatgaag tactgggcga
    19381 tgggaaaaaa gtcacaggag ccatgattcg taaccttaaa actggtgaaa ctgatcaact
    19441 ggcaatggat ggtattttta tagccattgg acacacacca aatactgcaa tatttcaaga
    19501 tcagctggct atgagagacg gttatatctt gattaagtca ggacttgatg gaatggcaac
    19561 aagcacttct gttcctggag tatttgcttg tggcgacgtt gctgatcatg tttatcgtca
    19621 agccattaca tcagcaggat ttgggtgcat ggcagccctg gatgctgaaa agtacctgga
    19681 ttcaatataa aattggctta cgatagcaac tatacttttc ctgacccgga aaccagtgat
    19741 aaacagggtt tattagcaat tgggggtgtt ttgaccccca agcgagtttt gcaagcctac
    19801 tcccaaggaa ttttcccttg gtatgagccc ggtaaccctg ttctttggtg gtcacctaat
    19861 ccgcgcctga tcttaatacc taacgaattt aaaatatcca gaagtttaaa aaaaactctg
    19921 aaaaaacctt ttaaactgac tgtcgatact gcttttcaac gagtcatttc atactgtgca
    19981 acttgttcag acaggtctaa taagacctgg atcactagtg aaatgataga aacatacacc
    20041 caattacatg agatgggata tgcgcattcc tttgaaatct gggatggttc cgaacttgta
    20101 ggcggactct atggaatcag ccttggacac gctttttttg gtgaatcgat gttccataca
    20161 ataacagatg cctccaaagt cgccttgcat tttttatgtc gcattatgca atcatggaat
    20221 tttgatttta ttgattgcca gttgccaacc ttgcatttaa tgagccttgg cgcaaaaata
    20281 ataagtcgaa aagaatttct tcatatgtta caagaaactt taaaataccc tgacaaaaag
//
LOCUS       DBNCWK010000046        19949 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.046, whole genome shotgun sequence.
ACCESSION   DBNCWK010000046 DBNCWK010000000
VERSION     DBNCWK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19949
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.046"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1113
                     /locus_tag="AC06IO_001469"
     CDS             <1..1113
                     /locus_tag="AC06IO_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874664.1"
                     /translation="YYLLELFVLINMAQSYDVDEQLKAIMSWLYQFNPILKASNKGLL
                     SFYAELEPKFHSEGHLEKRVETSTDDLLYRIKTFLVSLFVTPFEVFPFSGKTISFWDI
                     KNVIFAEGQEIYNQFTPFIMTNKLDALIAVYKKIMDEHIIPCQKNKHIYKWLTHYQST
                     LNWYQLVEAGDLSKMDVYWFDPELLFHVLVHFRLINKSLGEKTVNFLDELIHTYAQNN
                     NEFQIQLRVNILFSRFLKSLDEQQRRKLILTLSLFDPVEAKSKFLTNCIHYVTNRLCQ
                     ISMHQLVSSPNFFGTYQCIDSKKLLISKTDVKQVSAILEAFKEMLHSLEERCNPEQLE
                     NMLIFLRNISRPILTVAEIEEAQQSARVIDYIGAPT"
     gene            1274..2443
                     /locus_tag="AC06IO_001470"
     CDS             1274..2443
                     /locus_tag="AC06IO_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2744 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874665.1"
                     /translation="MSNLIELALRNAALDRTKLLSQVQKIEFLNGYDPRFVEGLISLE
                     MIDDIDEYVASLMYVKMTDKYTQEEKEQLTDTILTINNDRLISCQYRFPQLYQKLMQD
                     QFLSTFFSSSHLLPNIAPGTNNQSYETVSTNNKMKMEPAKLYGHYEYYQCVVALITQI
                     DGSRYFYHLNPNRIFGDYMGSAPLKLSGSELKDVKDITFILHPRSMVDYREFQRLQER
                     NIVFKTVMLPVGAKSVSLSYDTDKDVLTVNNLEAGQTITYNNLLQTPQYNRYQYTPEQ
                     YFMTQLYRGQTWAEALGEALDKYIVTRQEGATWWDLKPLFFNRETTKNNVIAAARIKK
                     EIEAGATSFDFTGVSRNGLLNQLLTTAVDYQLEYQRGLQNGVLSAEDLTTSAALR"
     gene            complement(2479..3456)
                     /gene="rsgA"
                     /locus_tag="AC06IO_001471"
     CDS             complement(2479..3456)
                     /gene="rsgA"
                     /locus_tag="AC06IO_001471"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HIO9874666.1"
                     /translation="MSKRRINKQQSARIAKIQKNYHENMGNNSDNLADGLVITRFSRH
                     AEIEDNQGKRILCSIRPNLDTLVAGDRVIWQIEGEKQGVVVSIYPRGSLLARPSQKGL
                     KKPVAANITQLVVVIAPKPEISWPLLDSYLIMAEILQLHVVIVLNKIDLPCQTLQQRL
                     MMDYQLLNYPVIMASNQTPDTIVPLKQALNNQISVFVGQSGVGKSSLISSLLPHEENI
                     AIDKISMVSELGKHTTRNSRYYHLPSGGALIDSPGVREFSLWDIDCAMIAQGYKEFKP
                     YLSQCKFRNCTHIDTPQCAIIDALNKGKISTQRYENFTKLCAQFNSLKE"
     gene            3519..4751
                     /locus_tag="AC06IO_001472"
     CDS             3519..4751
                     /locus_tag="AC06IO_001472"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HIO9874667.1"
                     /translation="MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGR
                     DFPVFLHPETKEEYALARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDE
                     QGNLIDPYQGQRDLEEKLLRHVSSAFAEDPVRVLRVARFASRFHHLGFRIANETRLLM
                     YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPN
                     PHQYHQEIDTGIHSLMTLRASSELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEE
                     GTKLIRALCARLRIPNDYRDLAVTVARAHLNIHRVCELRPNTIVKLLEQVDAFRRPQL
                     FHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVTPQTFIVQGYEGKAIKEAMHQ
                     SRVACVERIMTSWKSNEK"
     gene            4741..5304
                     /gene="orn"
                     /locus_tag="AC06IO_001473"
     CDS             4741..5304
                     /gene="orn"
                     /locus_tag="AC06IO_001473"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIO9874668.1"
                     /translation="MKNNQNLIWIDLEMTGLEPEQDRIIEMATIVTDPQLNILAEGPV
                     IAVSQPKILLDSMDAWNTKQHNQSGLVKRVLESNVSESQAEQLTIEFLKQYVDKGKSP
                     MCGNSICQDRRFLYKYMPELAAYFHYRNLDVSSLKELVLRWRPELMNGVVKESKHLAL
                     DDIKDSINELIYYRQHFINLPEVKNDK"
     gene            5294..5956
                     /locus_tag="AC06IO_001474"
     CDS             5294..5956
                     /locus_tag="AC06IO_001474"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIO9874669.1"
                     /translation="MTNRERLELPNGAKKLLLHSCCAPCSGEVMEALIFSEIDFTIFF
                     YNPNIHPLQEYEIRKEENIKFAQKHNIPFIDADYDKENWFARAKGLEWEPERGKRCTM
                     CFDMRFERTALYAYEHDFPVISSSLGISRWKDMNQINDCGVRAASHYPDLIYWTYNWR
                     KNGGSERMYEIAKREAFYKQEYCGCVYSLRDTNAWRKQNTRDRIKIGLNYYSESKNEG
                     EL"
     gene            5953..6876
                     /locus_tag="AC06IO_001475"
     CDS             5953..6876
                     /locus_tag="AC06IO_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIO9874670.1"
                     /translation="MSKHSHDGAHHHEHGEVTYDRAFIIAITANGLFVVMQIIFAYLA
                     NSTSLLADAFHNLGDVLGLILAWVATGLMKRKPTHKATYGFKKTSILAALANGALLVF
                     TCGIIATDAMYKLFSPTEIQAVSVMIVAAIGIVVNFSTALLFLRGSDDLNIRGAYLHL
                     FYDALISVGVVLSAGVLYLTGWLWIDPVVGLLIALVILKGTWSLFADSFRLIIDGVPR
                     DVSWIKVSDFLLAIPGVKSIHDLHIWAISTKENALSVHLFMPEESLSDDLRRELVEKL
                     KHDFSIQHVTIQVEKTEAECNDACHNPRIII"
     gene            complement(7012..8199)
                     /locus_tag="AC06IO_001476"
     CDS             complement(7012..8199)
                     /locus_tag="AC06IO_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIO9874671.1"
                     /translation="MIRVLFAFIILLLSVFLGIQLNKDPGYVLIAINNWTIETTLWVS
                     VFGLFFLFFLIYGLVQLCNKITRMPGMVNKWHSRRLAQKAQATTRKGLIEYSEGYWQK
                     AKNHLIQALPNTDTPLLNYLTAARAAQKMGDSKLRDHYLREAQQSMPEAKIAVELTQA
                     QLQLANHQWEQALATLKHLQDLAPRHPYVLKLLMNLYEEIKDWQQLITLLPDLKKNHI
                     ISDQAFEQLQKNTYLQAMIDLAKHNQSDGITKLYQTLPKTLVNDTAITAEYTRFLIRN
                     RDYDQAEYHLRRCLRKELDNRLIELYSLLPCEENQLLFAESLLKKNPHSAALHLCLGR
                     MCLSRHLWGKAKHYLEQSIELNPTPVAFIELGKLHEKLNDPLSSGSCYKKGLELITKD
                     ELS"
     gene            complement(8202..9326)
                     /locus_tag="AC06IO_001477"
     CDS             complement(8202..9326)
                     /locus_tag="AC06IO_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIO9874672.1"
                     /translation="MANSNEEQKKTIQPQQPSKVEPDINQQMKASFFGSKYLSLIISL
                     TSLLAFLAIIVSIYSIQLEKQFQNHQLVENKNLTDELERIKAEQNTVQKLLDNNANHL
                     QQIQSDLTSKIDSLNKELQTAMKQKLYQNNDWLLLKARYYLELAQINVHWSDNFNTSV
                     ALLQQADALLKGMNIPKIFTIRQTIAKEIAHLKSISVVDITGILSQLDAAQAAINNLS
                     IQSVVDQQEVLNHITKSEESGKTGWRNRLQDSVSFLEKLVVIRRHNENIQPLISPLYE
                     SAIKENIRLNLQEAQWAILNNNPAVYQFALNQAIANLKRVFNDSSQNTDALIKQLSTL
                     QNVKLTQEKPVVGQAIPLINQMIDNKELLVDQANSKKGEN"
     gene            complement(9330..10082)
                     /locus_tag="AC06IO_001478"
     CDS             complement(9330..10082)
                     /locus_tag="AC06IO_001478"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIO9874673.1"
                     /translation="MTKSLNGLRILNTRPREQAEVLNKKIIESGGIPIDCPTMEIVAS
                     ETNWINKLPDLTSVNQAIFVSGNAVRYFMMELASKRINWPNSIRVAAIGKGTSRVLNE
                     FNIQVRDVPELPDSEHLLTLNSLQQIRNQTILLVKGEGGRQLIEEYLMLKGAKLCLLS
                     VYKRVMPAIDQEFLNSLWRDDLADIILLTSEQSIHNLFKMFSIEGQMWLQNKPCLVIS
                     DRLAKVASLFGIKKILISHPERMIGTLFDYKD"
     gene            complement(10079..11008)
                     /gene="hemC"
                     /locus_tag="AC06IO_001479"
     CDS             complement(10079..11008)
                     /gene="hemC"
                     /locus_tag="AC06IO_001479"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIO9874674.1"
                     /translation="MSAKIIRIATRQSPLALWQANHVREMLVKQWPNLSIELLPMITS
                     GDRFLKDKLLSAGGKGLFVKELEEALLDKRADLAVHSTKDMPAQLPDGLSLAAICKRD
                     NPFDALISPQFKSLDALPKNAIIGTSSLRRQSQLLAYNPNLQIKTLRGNIHTRLSKLE
                     SGEYQAIILAAAGLERMGLTHHITQLIPDDIMLPTCAQGALCIECRTDDLEIQELIHS
                     LNDPISALCVHTERRVNAKLGGNCHIPFAVYCTITAENLLLLRAKVLNLDGSQMIDDE
                     QQGKIEEAEIIADRCTESLMTKGAMTLLSTIPS"
     gene            11399..12688
                     /locus_tag="AC06IO_001480"
     CDS             11399..12688
                     /locus_tag="AC06IO_001480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIO9874675.1"
                     /translation="MSQLKKIVKHLDESMQRSLSNSAHQLVAVGAIAFVGFPLFYVIW
                     AFWLPQPYENLPLRLIGSLLGLGLMLTPYWPLKWKQYLSWYWFLTLLFTLPYFFTFLF
                     LMNQASVISAMSLLCGVFLLVLLVDLLSLSIVLILGFSLALVSYYLVSPQMYFGEEHI
                     QMTLVIIFTIIAGSTLNYKTAMLQQQKLAGMAAAAGMIAHELRSPLLGIKSGAQALVQ
                     YLPRLIDGYQLAKKQELISSPLRDRLLLQLGGVSNRIVSEIDYANTIIDMLLVKASRD
                     NILQNCILETCSIAECLSEAITRYPFQSPRERTLITWTGDFQFKGSKLLMQHILFNLI
                     KNALYVIAMAQRGEINIWTESGDKYHYLYFKDTAKGMSKQQLNRLFNHFYTTTFMGTG
                     IGLSFCKLVMNAFGGDIVCESKEGEYALFILSFPGSA"
     gene            complement(12776..13339)
                     /locus_tag="AC06IO_001481"
     CDS             complement(12776..13339)
                     /locus_tag="AC06IO_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIO9874676.1"
                     /translation="MANSQEFYQPPVDELKRNWGWILGFGIFFLILGCVGLGMVVGLT
                     LVSMFFFGALLIVGGISHIIDVFKYKEWKGMIWQALIAVLYIAAGCIVLYDPFLASTL
                     ITAFLAAVLIVIGLTRMIMAFALKDSKGWGWLLLAGITAMILGILILMQWPVSGLWII
                     GLFIAIELIVTGWTYIFIAISVKTAKL"
     gene            13621..14646
                     /locus_tag="AC06IO_001482"
     CDS             13621..14646
                     /locus_tag="AC06IO_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIO9874677.1"
                     /translation="MQHFSIPTCYFPSTAVFVDDSRDFLLNFVLQLDEGLAYRVFDSP
                     FEALDCIKQKRCELELLSERCLSEYTEAKNCPLTNHTINLDLAAIHAEVYNSRRFSEI
                     SVVVVDYAMPGMDGLEFCRRIEDTNIKKILLTGQADEKLAIAAFNEGLIHRYIKKSDP
                     DVASLITQSIHDLQLQYFQSMSDMIVRMLSVTSPNCLHDKKYAELFWRLCREKGIVEF
                     YLADNSGSFLMLDDDANISFLIVKNEADMQLHYDLALDNGASGDVLDQLHNGEKIPCF
                     WQSNLVTPQWNEWSNCLVPANRFVSDETYFYAYVQGAVLFDVRLDKILSYHQYLEELD
                     AEEMFLN"
     gene            complement(14728..15978)
                     /gene="lqsA"
                     /locus_tag="AC06IO_001483"
     CDS             complement(14728..15978)
                     /gene="lqsA"
                     /locus_tag="AC06IO_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing autoinducer LAI-1 synthase LqsA"
                     /protein_id="HIO9874678.1"
                     /translation="MSNTEYQTIDNKTQYINGKEKHQNHAYPEYINHSMEKYYEGRVN
                     KSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLIKAQIAAMQKYGNGQMQSAVFLN
                     NSSFLLSQCEKKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
                     GVKAARAKPVPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFK
                     CLLIVDESHSLGTHGSQGKGMVAKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEY
                     IRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNADFLREALSCEGINIG
                     SSGSQIIPLVTGSEANTLWLRDELEKEDLFGAVFCSPATPKNKCLIRLSISAHHEKSD
                     LLRVIECLVRLARKRPDIPLFKGN"
     gene            16167..16595
                     /locus_tag="AC06IO_001484"
     CDS             16167..16595
                     /locus_tag="AC06IO_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIO9874679.1"
                     /translation="MRLNIALILSFFSIMLFAMDEVDEQQILQRIQPVGKVRVQEEQG
                     NNIEAEATKTTVVDSKSKKEPGQDIYDKYCSVCHRDGLAGAPKFQSEADWKPRLASKK
                     IDDLLASAIKGLNAMPAKGTCMDCSDAELKAAIQYMLPKS"
     gene            16592..17083
                     /locus_tag="AC06IO_001485"
     CDS             16592..17083
                     /locus_tag="AC06IO_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIO9874680.1"
                     /translation="MKKLTYRKIQSFQAIITVLVIFASFYLEYAVGLQPCPLCLMQRV
                     CVFILLGLMGVSLGTVKKAHIVSLMQFLVACAGLYFSLRQLWLQSLPSDQAPACMPGL
                     DVLIQYFPWQTVAKALFWGAGDCAEVTWTMFGISMPGWAAMYFLFMAIMGLFLFFRTR
                     TIN"
     gene            17145..18296
                     /gene="tgt"
                     /locus_tag="AC06IO_001486"
     CDS             17145..18296
                     /gene="tgt"
                     /locus_tag="AC06IO_001486"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIO9874681.1"
                     /translation="MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVN
                     NMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVF
                     SLSKNKEICSIDESGAHFKLPDSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
                     KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSA
                     MNTDGIAIGGETVGFDMDTTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGID
                     MFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENGRIQIKKSCFADDLAPVMESCS
                     CYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS"
     gene            18323..18817
                     /locus_tag="AC06IO_001487"
     CDS             18323..18817
                     /locus_tag="AC06IO_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIO9874682.1"
                     /translation="MVLISTSMGDIHIKLDTENTPLTAENFLNYVRKGFYNDTIFHRV
                     IDGFMIQGGGLNTNMEQKTTASPIQNEAKGAKPNKRGTIAMARTMDPHSATAQFFINV
                     ADNGFLNYSGDHPQGYGYCVFGEVVEGMDVVDAIAKVKTGQRNGHADVPVEEISIIEV
                     RELA"
     gene            complement(19026..19655)
                     /locus_tag="AC06IO_001488"
     CDS             complement(19026..19655)
                     /locus_tag="AC06IO_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIO9874683.1"
                     /translation="MLLHYLFIVGICVEAITGALAAGRKKMDFFGVLLIASITALGGG
                     TVRDTLFNTYPLTWVAHPEYLLYTSVCAFLTIFIAHSLVRIMKVFLILDALGLSTFVI
                     IGTQKVLAHGMPPSVAVISGMITGICGGMLRDILCNDIPLVLRKELYAVIALAGALLF
                     ITLDYFHIPHNLNIIITLLCIFTARLLAIFFHIEIPIFDYSESIKRRKK"
BASE COUNT         5966 a         3447 c         4061 g         6475 t
ORIGIN      
        1 tactatctat tggaactttt tgttttaatc aacatggcgc aatcttatga tgtagatgaa
       61 cagttaaaag ctataatgag ttggctgtat caatttaatc ctatattaaa ggcatcaaat
      121 aaagggttac tatcttttta tgcagaattg gaaccaaaat tccactcgga aggtcactta
      181 gagaagagag tagaaacttc gacagatgat ttattgtatc gtattaaaac ttttttagtg
      241 tcattatttg ttactccatt cgaagttttt cctttttcag gtaaaacaat cagtttttgg
      301 gatatcaaaa atgtaatctt tgcagaagga caagagatct ataatcagtt tactcctttc
      361 ataatgacga acaaattaga cgctttaatt gcagtctata aaaagataat ggatgagcac
      421 attatccctt gccaaaaaaa taaacacatt tacaaatggc tgacacacta tcaatcaacg
      481 ctgaattggt atcagctggt agaggcaggt gatttatcta aaatggatgt ttattggttt
      541 gacccggaat tattatttca tgttttggta cattttagat tgattaataa atcattgggc
      601 gaaaaaactg ttaatttttt ggatgaatta atacatactt atgctcaaaa taataatgag
      661 tttcaaattc aactgagagt caatatttta ttttccagat tcttgaagag tttggatgag
      721 caacagagac gaaaattaat tttaactttg tcattgtttg atcctgtaga ggctaaaagt
      781 aaatttttaa cgaactgcat tcattatgtg acaaaccgac tatgtcaaat tagcatgcat
      841 cagttagtca gttcacctaa tttttttggc acttaccaat gcatagatag taagaaattg
      901 ttaattagta aaacagatgt aaagcaagtg agtgctattt tggaagcctt taaagaaatg
      961 cttcattcgt tagaagagcg ttgtaaccct gagcaattgg aaaatatgct cattttcctg
     1021 cgaaatataa gccgccctat tttgactgtt gcggaaatag aagaggcgca acaatcagcc
     1081 cgagttatcg attatatagg cgctccgacc tgacttaatt aactggaatt atcctaattt
     1141 attattgtgc attttgattt catcgcgttg ataaggattg attgtcttgg cctggatgag
     1201 caaagcttgc tccagtgtac gaattgaatt ataattagcc aatttgtctc atttatgctt
     1261 ttaaaattat aatatgtcaa atctaataga acttgcttta agaaacgcgg cacttgatag
     1321 aacaaaactt ttaagtcagg tacaaaaaat tgaattttta aatggttatg atcctaggtt
     1381 tgtcgaggga ttgatatcat tagaaatgat agacgatatt gatgaatacg ttgcttcttt
     1441 gatgtatgtt aaaatgactg ataaatatac tcaggaggaa aaagagcaat taacagatac
     1501 tatattaacc atcaataatg atcgattgat ttcatgccaa tatcgctttc ctcaattata
     1561 tcaaaaatta atgcaagacc aatttttatc tactttcttt tcaagttcgc atttgctccc
     1621 caatattgct ccaggcacaa ataatcaatc ttatgaaaca gtaagtacta ataataaaat
     1681 gaagatggag ccagcaaagt tatatgggca ttatgaatat tatcaatgtg ttgtcgcttt
     1741 aatcactcaa attgatggtt ctcgatattt ttatcatttg aatccaaacc gtatctttgg
     1801 tgattacatg ggtagtgcgc ctttgaaatt atcaggtagt gaacttaaag atgtaaagga
     1861 tataaccttt atccttcacc cgcgaagtat ggttgattat cgcgaatttc aaagattaca
     1921 agagagaaat attgttttta aaaccgttat gctaccagtg ggggcaaagt cggtatcact
     1981 tagttatgat acagataaag atgtattgac ggttaacaat cttgaggcag gacaaactat
     2041 aacatataac aatctgctac aaacacccca atataataga tatcaatata cacctgagca
     2101 atatttcatg acccaactat atagaggtca aacatgggca gaagcacttg gcgaggcatt
     2161 ggataaatat attgttacaa gacaagaagg agcaacctgg tgggatctta aaccattatt
     2221 ttttaatcgt gagactacaa aaaataatgt gattgctgct gcgcgaatca aaaaggaaat
     2281 tgaggctgga gctacctcat ttgattttac aggggttagc aggaatggct tgctgaatca
     2341 attattaaca actgctgttg actatcaatt agaataccag cgtgggttgc aaaacggggt
     2401 gctttcggct gaagacctaa caacaagtgc tgcgttacgt tgataactga ttatccattt
     2461 ttcggaagag ctttttgttt actcttttaa actattaaat tgggcacata attttgtaaa
     2521 attttcatat cgttgggttg aaatctttcc cttatttagc gcgtcaatga tcgcgcattg
     2581 aggcgtatca atatgtgtgc agttgcggaa tttacattgg gacaaataag gtttgaattc
     2641 tttatatcct tgagcaatca tagcgcaatc aatatcccac aagctgaatt cccgaacgcc
     2701 tggcgagtct atcaaggcac caccactagg tagatgataa tagcgagagt ttcttgtagt
     2761 atgtttgcct aactctgaaa ccatggatat tttatcgatc gcaatatttt cctcatgtgg
     2821 caataaactt gaaatcaatg atgatttgcc tacccctgat tggccaacaa aaacactgat
     2881 ttgattattt aatgcttgtt ttaatggtac tattgtgtct ggggtttggt tacttgccat
     2941 gatgacggga tagttcagca attggtaatc catcatgagt cgctgctgaa gtgtttgaca
     3001 gggaagatca attttattga gaacgatcac cacgtgtaat tgaagtatct ctgccataat
     3061 caaatagctg tcaagtaatg gccaactaat ctctggtttt ggagcaatca ctacaaccag
     3121 ttgagtgata tttgccgcaa cgggtttctt taaccccttc tgactgggcc ttgccagtaa
     3181 gcttcccctg ggatagatgc tcacaactac cccttgtttc tcaccttcta tctgccagat
     3241 tactctatca ccagccacca gtgtgtcaag attaggtcgg atagagcaaa gaattcgttt
     3301 cccctgattg tcttcaattt ctgcatgcct gctaaatcga gtaatgacta aaccatcagc
     3361 gaggttatcg gagttatttc ccatgttttc atggtagttt ttctggattt tagctatacg
     3421 ggcagattgc tgcttattga ttcgtctttt actcatagtg tcaatctata ctataattga
     3481 tgcgattaat aatacagtat gaaggtaaat catatttaat gaaagtctac ttggtaggcg
     3541 gtgcagttcg tgatcgatta ttaggaattc ctgtccagga acaggattgg gtagtcgtcg
     3601 gagcaacgcc ggaagaatta ttaaaaagaa aatataggca agttgggcgg gatttccccg
     3661 tttttttgca tcctgaaacg aaagaggaat atgcattagc aagaacagag cgtaaatcag
     3721 ctccaggata ttatggattc atttgtgatt ttagtgaatc agtgacgttg gaagaggatt
     3781 tggcacgacg agatttaacg attaatgcca tggctatgga tgaacaggga aatttaattg
     3841 atccatatca gggacagcga gatctcgaag agaaattatt acgtcatgtg tcttccgctt
     3901 ttgcagaaga tcctgttcgg gttttacgtg ttgcccgttt tgccagtcgg tttcatcatc
     3961 ttggatttag aatagccaat gagactcgtt tattaatgta ttcgatggtt aaacaaggtg
     4021 aattagctca tttgatcccc gagcgtgttt ggcaggaatg gcaaaaaagt cttgaagaaa
     4081 aaaatcctga acaattcatt ctttctttac gttcctgtga tgcattaaga gtgattttgc
     4141 cagaaattaa ctcattattt ggtgtgccta atccacatca ataccatcaa gaaatagata
     4201 cgggcattca ttcattaatg acattacggg catcatctga gcttagcgaa gaaccattag
     4261 tacgatttgc tgctttggtt catgatttag gcaaagcctc gacaccaatt caggcatggc
     4321 ctaaacacca tgggcatgag gaggagggta ccaagctgat tcgagcatta tgtgctcgct
     4381 tgcgcattcc taatgattat cgcgacttgg ccgttacagt ggcgcgagct catttgaata
     4441 ttcatagggt atgcgaattg cgtcctaaca caatcgtcaa gcttttggag caggtagatg
     4501 cctttagacg acctcaatta tttcataaaa tattgattgc ttgtcaagca gatgccgaga
     4561 gttgtggcaa aacagtggtc tatcgccaaa ctcaattatg gaatgaaata ttatctgaat
     4621 gtgttaaagt gacgccacaa acttttatcg tacaaggtta tgaaggtaaa gcgattaagg
     4681 aggccatgca tcaaagtcgt gtggcttgtg tagagcgaat tatgacttct tggaaatcaa
     4741 atgaaaaata atcaaaattt aatttggatt gatttggaaa tgactggcct tgagccagaa
     4801 caagatagaa ttatagaaat ggccacgatt gtaacggatc cacaacttaa cattctcgca
     4861 gagggacctg tcatagcggt gtctcagcct aaaattcttc tagacagtat ggatgcatgg
     4921 aatacaaagc aacataatca gtcgggattg gtgaagcgag ttttagagag caatgtcagt
     4981 gaatctcaag ctgaacaatt aacgattgaa tttttaaaac agtatgtgga taagggaaaa
     5041 tcacccatgt gtggcaatag tatctgccag gatagacgtt ttttatataa atacatgcct
     5101 gagttggctg cttatttcca ctatcggaat ttggatgtca gttcgttgaa agagctggta
     5161 ttgcgctggc gtccagaatt aatgaatgga gtagttaagg aatcgaaaca tcttgctttg
     5221 gatgatataa aagattcaat caatgagctg atttattatc gacaacattt tattaatttg
     5281 ccggaagtaa aaaatgacaa atagagaacg tttagaatta cctaatggcg ctaaaaagtt
     5341 attacttcat tcctgttgtg ctccttgttc aggtgaagtg atggaagcgc tcattttttc
     5401 tgagattgac tttactatat ttttttataa ccccaatatt catcctctcc aggaatatga
     5461 aataagaaaa gaagagaata ttaagtttgc ccaaaagcat aatattccct ttatcgatgc
     5521 cgattacgat aaagaaaact ggtttgctcg agccaaagga ttggaatggg agccagaaag
     5581 aggcaagcgt tgcactatgt gttttgatat gcgatttgaa agaacagcac tctatgctta
     5641 tgagcatgac tttcctgtta tcagtagctc tttgggtatt tcacgttgga aagatatgaa
     5701 tcagatcaat gattgcggag tgcgggcagc aagtcattat cctgatttga tatattggac
     5761 ttataattgg cgaaagaatg gcggctctga aagaatgtac gaaattgcca aaagagaagc
     5821 attttacaaa caagagtatt gtggttgtgt ttattcttta cgagatacga atgcctggcg
     5881 caagcaaaat acaagagatc gtattaaaat tggacttaat tattactctg aaagcaagaa
     5941 tgaaggagaa ttatgagtaa gcattcgcat gatggagcac accatcatga gcatggggaa
     6001 gtgacttatg acagggcttt tataattgca attactgcga atggcctttt tgttgtgatg
     6061 caaattattt ttgcctattt agcgaattct accagtttgc tagcagatgc ttttcataat
     6121 ctaggtgatg tattagggtt gattttagca tgggtagcaa ccggattaat gaagagaaaa
     6181 cctacccata aagcaactta tggatttaaa aaaacctcta ttttagcggc attggccaat
     6241 ggagcgttac tggtatttac ttgcgggatc atagcgacag atgctatgta taagttattt
     6301 tcaccgactg aaattcaagc cgtctctgtg atgatagtcg ccgcgattgg tatagtcgtg
     6361 aacttctcta cagcgctctt gtttttacgg ggttccgatg atctcaatat tcgaggagct
     6421 tatttacatt tattttatga tgccttgatc tcggtaggtg ttgttttatc agcgggtgta
     6481 ttatacctga cgggttggtt atggatagat ccggttgtcg gactattaat tgcattagtg
     6541 attttgaagg gcacttggtc actatttgcc gatagtttca gattaattat tgatggtgtt
     6601 ccccgtgatg tatcctggat taaagtcagt gattttctct tggctatacc tggcgtaaaa
     6661 tctattcatg atttacatat ttgggcaatc agtacgaaag aaaatgcact ttctgttcat
     6721 ttatttatgc cggaggagtc attatctgat gatttgcgta gagagctggt tgaaaaatta
     6781 aagcatgatt tcagcattca gcatgtcact atccaagttg aaaaaacaga agcagaatgc
     6841 aatgacgcat gtcataatcc caggataatc atttaattga ggctggattt acgaccccta
     6901 atttatttgt ttaaaaaagg gtttaattga ttcattaatt gtatctaaac gcccttatta
     6961 aaaacatttt ttgccttgca tttcgagctg tgtctttcag tttttaatca attaggataa
     7021 ttcatctttt gttatcaact ccagaccttt tttataacaa gagccactgg ataaggggtc
     7081 atttagcttt tcatggagtt tgccaagttc gatgaatgct acgggcgtgg ggtttagctc
     7141 aatagactgt tcaagataat gtttggcttt tccccataaa tgccgcgaga ggcacattct
     7201 ccctaaacat aagtgcaatg cggctgagtg aggatttttt ttgagtaatg attcagcaaa
     7261 caacaattga ttttcttcac aagggagcag gctataaagc tcaattaacc gattatccaa
     7321 ctctttgcga aggcatcgcc ttaaatgata ttctgcctga tcgtaatccc gattcctaat
     7381 cagaaagcgg gtatattctg ctgtaattgc tgtatcgtta accagtgttt ttggcagcgt
     7441 ctgatacaat ttggtaatgc catctgattg attgtgtttt gccaaatcaa tcatggcttg
     7501 caaataagta tttttttgta attgttcaaa agcttgatct gaaataatgt ggttcttttt
     7561 taaatcaggt aaaagagtga tcaactgctg ccaatctttt atttcttcat aaagattcat
     7621 taataatttc aagacataag gatggcgtgg tgctaaatct tgaagatgct ttaatgtggc
     7681 caatgcctgt tcccattggt gattggccaa ttgtaattgc gcctgggtta attcaacagc
     7741 aattttcgct tcaggcatag attgctgtgc ttccctaaga taatgatcgc gaagcttgct
     7801 atcccccatt ttttgagcgg ctctcgctgc tgttaaataa ttaagtaaag gagtgtcggt
     7861 gtttggcaga gcttgtatta aatgattttt tgctttttgc caatagccct cgctatattc
     7921 tatcagtccc tttcgagtcg tggcttgagc tttttgtgcc aagcgtcgtg agtgccattt
     7981 attgaccatg cctggcattc ttgtaatttt attgcaaagc tgaaccaggc catagattaa
     8041 aaaaaacaga aaaaacaagc caaaaacaga gacccataag gtggtttcta tagtccagtt
     8101 attaatagct attaagacat aacctggatc cttattgagt tgaataccaa ggaacacaga
     8161 gagcagtaaa ataataaaag caaacagaac tcgtatcatt cttagttttc tcctttcttg
     8221 ctgtttgctt gatcgaccag caattcttta ttgtcaatca tttgattgat tagcgggatg
     8281 gcttgtccta cgacaggttt ttcctgagtt aattttacat tttgcagagt ggacaattgc
     8341 ttaatcaaag cgtcagtgtt ttgggaagag tcattaaata ctcttttaag atttgcaatg
     8401 gcctggttta aagcaaactg gtagactgcg gggttattat ttaaaatagc ccactgcgct
     8461 tcctgaaggt taaggcgtat attctctttt attgcagatt catatagagg ggagataagt
     8521 ggttgaatat tctcattgtg gcgacgaata acaaccaact tctccagaaa actcacgctg
     8581 tcttgcaacc gatttcgcca tccagttttg ccagactctt cagacttggt gatatggttt
     8641 agcacttcct gttgatctac tacggattga atagaaagat tattgatagc cgcttgagcc
     8701 gcatccaatt ggcttaatat ccctgtgata tcaacaacag aaattgactt taaatgggca
     8761 atttctttgg cgattgtctg gcgaatggta aatattttgg ggatattcat gcctttcagt
     8821 agggcatcag cttgctgtaa aagggcgaca gaggtattaa aattatcact ccaatgaaca
     8881 ttgatttgtg ctaattcaag ataataacgt gcttttaaaa gaagccaatc attattttga
     8941 tacaattttt gcttcatagc tgtttgtaat tctttattta agctatctat tttgcttgtt
     9001 aaatcactct gaatttgctg aagatgattg gcattgttat ccaggagctt ttggactgta
     9061 ttttgctctg cttttattct ttccaattca tcagttaaat ttttgttttc aacaagctgg
     9121 tggttttgaa attgtttctc caattgaatg gaataaatag agacgatgat agcaaggaac
     9181 gccaaaagcg atgtcaagct gattattaag gataggtatt tactaccgaa gaaagaggct
     9241 ttcatttgtt ggttgatgtc gggctcaact ttagatggtt gttgcggttg aattgttttt
     9301 ttctgttctt cattgctatt ggccatggat taatccttat agtcaaataa cgtacctatc
     9361 atcctttctg gatggctaat aagaatcttt ttaattccga ataaagaagc tacctttgca
     9421 agtcggtcac taatgaccag gcaaggtttg ttttgtaacc acatttgccc ctctatgcta
     9481 aacattttaa aaagattatg tatggactgc tcacttgtta acagtataat gtccgccagg
     9541 tcatcgcgcc acagagaatt aagaaattct tggtcgatgg ctggcatgac tcgtttatag
     9601 actgatagaa gacatagctt tgctcccttc agcattaaat attcttcaat cagttgcctg
     9661 cctccttctc ctttcacaag cagaatcgtc tgatttctta tttgttgcaa cgaattgaga
     9721 gtcaataaat gctcgctgtc aggtaattcg gggacatctc taacctggat attgaattca
     9781 ttgagtactc tggaggtgcc tttacctata gcagctaccc ggatgctatt aggccaattg
     9841 attcttttgg aagccagttc catcatgaaa taacgaactg catttccact gacaaaaatt
     9901 gcctgattta ctgaggtcaa atcaggcaat ttatttatcc aattggtctc agacgccact
     9961 atttccatcg tagggcagtc gatgggtatc ccgccagact cgataatttt tttatttagt
    10021 acttctgctt gctcccttgg cctggtattt aatatgcgaa gaccattaag tgatttggtc
    10081 atgatggtat cgtgctcagt aaggtcatcg caccttttgt cattaatgac tcggtacacc
    10141 tgtctgcaat aatttcagct tcttctattt tcccttgttg ctcatcatca atcatttgag
    10201 aaccatccag attaagtact tttgctctca aaaggaggag gttctccgca gtaatggtac
    10261 aataaacagc aaagggtata tggcaattgc ctcctaattt tgcgttgacg cgtcgctcgg
    10321 tatgaacaca aagagcagaa attggatcat ttaagctatg aatcagctct tggatttcca
    10381 aatcgtcagt tcggcactca atacataaag cgccctgagc acaagtaggc agcataatgt
    10441 catcgggtat gagttgggtg atgtgatgtg tcaagcccat tctttccaag cctgcggcag
    10501 caagaataat ggcttgatac tctcctgact ccagtttgct gagtcttgta tggatgtttc
    10561 ctcttaatgt tttgatttgc aaattgggat tgtaagccaa tagttgagat tgacgtctaa
    10621 gactcgatgt accaataatt gcgtttttgg ggagagcatc cagactctta aattggggac
    10681 ttattaaagc gtcaaaaggg ttatctcttt tgcaaatggc ggccaacgag agaccatccg
    10741 gaagttgtgc tggcatgtct ttggtcgaat gaacagccaa gtccgctctt ttatccaata
    10801 atgcttcttc taactcttta acaaataacc ctttccctcc tgcggataat aatttatctt
    10861 taagaaatcg atcgccggag gttatcatgg gtaataattc aatgcttaga tttggccatt
    10921 gctttacaag catttcccgt acatgattag cctgccatag tgcgagcggg ctctgcctcg
    10981 ttgcgattcg tataattttt gcgctcatgg aatagacggt atatgttctt tgggtaaaga
    11041 tcataacatt gattctaatt taaaaagaac cccattgaga taataggtac gcaaatcttg
    11101 tcgggcaatt gatttataaa tgagcattcc tactggtttg agaatggact tatagtgcca
    11161 acccccgatt tcttcgctaa aaaatttttt aatcctgtca tttatagtca tttaacaatc
    11221 aaataactat agcttatcta agctccttat taaaaatttt ttttgcctcg accttagagc
    11281 tttgtgtaag tcctatagag catatctcat gcacaatccc tatattttat gataggcttt
    11341 tccagtagat aaagcgctga aaatcacggg cgtttttagc ttgatttaag gataaggaat
    11401 gtcacaacta aaaaaaatag tgaaacattt agatgaatca atgcagcgta gtttgtctaa
    11461 ttctgctcat caattggttg cagtaggtgc gatagctttt gtgggatttc ctttatttta
    11521 tgttatttgg gctttctggt tgcctcagcc ttatgaaaac ctgccactcc gtcttattgg
    11581 aagcctttta ggtttgggat tgatgcttac tccttattgg ccattaaaat ggaagcaata
    11641 tttatcttgg tattggtttt taactctttt gtttactttg ccatattttt ttactttttt
    11701 atttttaatg aatcaagcaa gcgttatttc tgcgatgtct ttgttgtgtg gtgtattttt
    11761 acttgtattg ctcgttgatt tattgagttt atccattgtg ctgatactgg ggtttagttt
    11821 agcccttgta agttattatc ttgtttctcc gcaaatgtat tttggagaag agcatataca
    11881 aatgacattg gtgatcatat ttaccattat tgcaggttct actttaaatt ataaaacagc
    11941 catgttgcaa caacaaaaat tggctggtat ggctgctgct gcaggcatga ttgcccatga
    12001 attgcgttca ccattgttag gaataaagag cggcgctcaa gctttagtcc aatatttgcc
    12061 aagattgatt gatggttatc aattggctaa aaaacaagag ttaatatcct cacctttacg
    12121 tgacagattg ttattacagc ttggaggagt cagcaatcga attgttagtg aaattgatta
    12181 tgctaatacg atcattgata tgttattagt caaggcaagt cgcgataata ttttgcaaaa
    12241 ttgcatatta gagacttgtt ctatagcaga atgtttgtct gaggctatta ccagataccc
    12301 atttcagtca ccaagagaaa gaacattaat cacatggact ggtgattttc aatttaaagg
    12361 ttctaagttg ttaatgcagc atattttatt taatttaata aaaaatgcac tttatgttat
    12421 cgcaatggca cagcgtgggg agattaatat ttggactgaa tctggcgata agtatcatta
    12481 tttgtatttt aaagacacag caaaagggat gagcaaacaa caattaaatc gattatttaa
    12541 tcatttctat actactacct ttatgggaac aggcattggt ttatcatttt gtaaattagt
    12601 tatgaatgcg tttggtggtg atattgtttg tgaatctaaa gagggcgaat acgctttgtt
    12661 tatactgagt tttccaggct cggcttaaaa ttggttttct gttgaacata ccatcattcg
    12721 tcttaaaaga cgtaaggatg gtattgagtt agagagctcc tgttattctc ttctgctaga
    12781 gtttggccgt ttttactgag atagcaataa aaatatatgt ccaaccagtg acgatcaatt
    12841 caatcgcaat aaaaagccca atgatccaca gcccgctaac tggccattgc attagaatta
    12901 atatcccaag aatcatggcg gtgattccgg ccaataacaa ccatccccat cctttggaat
    12961 cttttaaggc aaaagccata atcattcttg ttaaaccaat tacaataagc accgctgcca
    13021 gaaaagctgt gattagagtg gatgctaaaa acgggtcata caaaacaatg catcccgccg
    13081 caatatacaa cactgcaata agcgcctgcc atatcattcc tttccactct ttgtatttaa
    13141 atacgtctat aatgtgactt attccgccaa caattaataa ggcaccgaaa aagaacatac
    13201 ttaccaaggt tagaccaacc accattccaa gaccaacaca gccaaggata aggaaaaata
    13261 ttccaaatcc taaaatccat ccccagtttc gttttaactc atccactggg ggttggtaaa
    13321 attcttgtga attagccata tcaatatcct tttttgattt cttagctaag ataaggtact
    13381 tatttaataa tatagactta tattcaataa cttgtctaat tttgagcttt gattttaaga
    13441 gcacttattt tgaatactta ctccaaaaag cctggtgaat ataaaacgta aataatttgg
    13501 ttataataaa caatcatgtt actttattca caagttagcc aggttctgat gatggagcca
    13561 caggttattt gaagttcata gaaaggatat aactttggaa cgttttgctc aggaggagct
    13621 atgcaacatt tctcaatacc tacctgttac tttccaagca ctgcggtttt tgtcgacgac
    13681 agtcgggatt tcttgctcaa tttcgtattg cagctggatg agggtttggc gtaccgtgta
    13741 tttgattccc cttttgaagc tttggattgt attaaacaaa aacgctgtga actggaacta
    13801 ttaagtgaac gttgtctcag cgaatacaca gaagcaaaaa attgcccatt aactaatcat
    13861 actattaatc ttgatttggc tgctattcat gccgaggttt ataactctcg acgattttct
    13921 gaaatttcag tagtcgttgt tgattatgca atgccaggca tggatggatt agagttttgc
    13981 aggcgaatcg aagatactaa tattaaaaaa atattattaa ctgggcaagc agatgagaag
    14041 ctggcaatcg ctgcctttaa tgaaggatta attcatagat acatcaaaaa gagtgatccg
    14101 gatgtcgcca gtctcattac tcaaagtatc catgatttgc agttacaata ttttcaaagc
    14161 atgtctgata tgattgtacg tatgctttcg gtgacatccc caaattgttt acatgacaaa
    14221 aaatatgctg agttattctg gcgtttatgc agggaaaagg gaatcgttga gttttatctg
    14281 gccgataact caggtagctt tttgatgctg gatgatgatg ccaacatcag ttttttaatc
    14341 gtgaaaaacg aagcggatat gcagttacac tatgatttgg ctttggataa tggagctagt
    14401 ggagatgtat tggaccaatt gcataatgga gaaaaaattc cttgtttttg gcaatctaat
    14461 ttagtgacac ctcaatggaa tgagtggtcg aattgtttgg ttcctgccaa ccgatttgtt
    14521 tctgatgaaa cttattttta tgcttatgtg caaggggctg ttttatttga tgtgcgcctg
    14581 gataaaattc tttcttatca ccaatacctg gaagagctgg atgcagaaga aatgtttctg
    14641 aattaataga aattctatat aaagagcaca taattcttat ttgaaatgtt aactgttcaa
    14701 atgagctgcc aataattaga agtcttgtta attaccctta aacaaaggaa tatcgggtct
    14761 ttttcttgca agtctgacca ggcactcaat cactctgagc aagtccgatt tttcgtgatg
    14821 cgcgctgatg gataaacgaa tcaggcattt atttttggga gtggcgggtg aacagaacac
    14881 ggctccaaag aggtcttctt tttctaactc atcccttaac caaagcgtgt tagcctcgct
    14941 gcctgtaact aaagggatta tttgagagcc actacttcct atattaattc cttcgcatga
    15001 taaggcttct cgtaaaaaat cagcattggt ttgtaatttt gctcttctcc attcttcttg
    15061 agtaataatt tctaatgatg cgctaaatcc tgctaaatca tgaggcatta aagcagaact
    15121 aaaaatagag ggtagagatg aatatctgat atattctata agttgcctat ctcctgtaat
    15181 gatgcctccc cgtcctgata aggctttagc caggctggct gttatgatgt ctatttggct
    15241 tgataatccc agtttagcta ccatcccctt gccttgcgaa ccatgcgtac ctaatgaatg
    15301 agattcatcg acgatgagca aacatttgaa ttttcttgca atgtcagcat agtcagccaa
    15361 tggactaatc gtacccagcg tactataaat agaatctact gcaattattc ctggcccatg
    15421 gtgctcaatt cgttttttta gtgaatcgac tgaattatgt agaaacggta caggtttggc
    15481 tcttgctgct tttacaccag accaaaaaga catatgggca taaaaatcca gatacactgg
    15541 tgtattgtac ttggccaggg tttgtatgag accaacgtta gcgctccagc ctgattgagt
    15601 gagcagtgcc gcagaataat ttaaaaagga actgaatttc ttttcacatt ggctgagcaa
    15661 aaaagagctg ttatttaaaa atacagcaga ttgcatttga ccattaccat atttttgcat
    15721 cgcagcaatt tgcgctttaa ttaattgcgg atgttgactg atgtttaaat aatcattgct
    15781 cgaaaaaatc agtgaatttg cagtcggggt tttgccttta agtatgtgtt tcccttgcca
    15841 ggatttattg actcgtccct cgtaatattt ttccatggaa tgattgatgt actcaggata
    15901 agcatggttt tgatgttttt cttttccatt gatatattga gtcttgttat caattgtttg
    15961 gtattctgta ttgctcatcc ataactcaga ttcttttccc ttgtgtgcag tttagcacaa
    16021 tggttgatag tggaaggatc ttttcagtaa aagtctaagt attaagttaa tgattttata
    16081 aatcgttggg cctattgaaa tctttctgga aaaaaaagtc acttgtcggt atgattttta
    16141 aattaactat tagaggacgg ataaatatga ggttgaatat tgcgctgatc ttgtcttttt
    16201 tttcaatcat gctttttgca atggacgagg tggatgagca acaaatttta caaagaattc
    16261 aaccggttgg gaaagttcga gttcaagagg aacaggggaa taatatagaa gctgaagcga
    16321 ctaaaacgac tgttgttgat tccaagagca aaaaagaacc aggccaagat atatatgata
    16381 aatattgctc cgtatgccat agggatggat tggcaggggc gcctaaattt caatcggagg
    16441 ctgattggaa acctcgattg gcaagtaaaa aaatagatga tttactcgct tctgcaatca
    16501 aggggttaaa tgccatgcca gcaaaaggta cttgtatgga ctgttctgat gctgaattaa
    16561 aagccgcaat acaatatatg ttgccgaaat catgaaaaag cttacttaca gaaaaataca
    16621 atcttttcag gcaattatta cagtattggt aatattcgct tcgttttacc ttgaatatgc
    16681 tgttgggcta caaccatgtc ctttgtgctt gatgcaacga gtttgtgtat ttattttgtt
    16741 agggttaatg ggggtgtctc tgggaaccgt gaaaaaagcg catatagtaa gcttgatgca
    16801 gtttctggtt gcgtgtgccg gtttgtattt ctctttaagg caattgtggt tgcaatcttt
    16861 accatcagat caagctcctg cttgcatgcc tgggcttgat gtattaattc agtattttcc
    16921 atggcaaact gttgctaaag ccctattttg gggagcaggg gattgtgctg aagtgacctg
    16981 gactatgttt ggaatttcta tgcctgggtg ggctgccatg tattttctgt ttatggcgat
    17041 aatggggctt tttctgtttt ttcgaacccg aacgatcaat taattcctgg cattaaacgc
    17101 gtgctttcgt tatcattctt tgttttatct tgtcgtttct gcgaatgaac actttttcat
    17161 gtgaagtact gtaccagcat tcgggtacca aagctcgtgt tacacgtgtg gttacgcctc
    17221 atggtgaatt tataactccg gtatttatgc cagttggaac ccgtgcaggg gtcaataaca
    17281 tgatgccgtc agaactgtct gctgcgggta ctcaaattat tctgggcggt aatacctatc
    17341 atatgttgtg tgcgcctggc atgaaggtga tagaaaatgc aggggggatg catcgcttta
    17401 tggggtggca tggacctatg ctaacagaca gcggtggttt tcaagttttt agtttatcga
    17461 agaacaagga aatttgttct atagatgaat ctggtgctca ttttaaattg cctgacagta
    17521 aggggattat ccatatgacc ccggaaatgt ctctggagac ccagaaaata attggtgccg
    17581 atattataat ggcatttgat cagtgtactc ctgatagctg ctccaaggat gaagtaagtc
    17641 aaatcatgtt gcggacccac agatggctaa aacaatcgat tgactaccat caacaatatc
    17701 ccaactctcg atacggatat aaacaagctt tatttggcat tgtgcaagga ggagtttttc
    17761 aagatttacg tcgagaaagt gctgattttg tttctgcaat gaatacggat ggtattgcaa
    17821 taggtggtga aactgtaggc tttgatatgg atactacagt ggaagtcatt cgttgggtgt
    17881 atgagtatct tccgggcaat aagccacgtt atactatggg agttggaatg tcacctcaag
    17941 atctgttgga tgtggtggca gaaggtatcg atatgtttga ttgtgttgca ccaacacgta
    18001 atgcacgaca tggtgcctta tattgtggta aattggtgag agaagggaat tggctaaaat
    18061 ttcaaagcga acatgaaaat ggacgaattc aaataaagaa atcatgtttt gctgatgatt
    18121 tggcacctgt tatggagagt tgttcttgtt atacttgtct taattattca agatcctatt
    18181 tgcatcattt atcaaaacaa aaagctaatt tatttactgc tttggcaagc atccataatg
    18241 ttcatgtttt gcatgatgtt tgtaatcaca tgcgtcattt gataataaat tcctaaatta
    18301 tttaatatta agaggtgaaa taatggtttt aatttctaca tccatgggtg atattcatat
    18361 caaattagat acagaaaata ccccattaac tgcagaaaac ttcctaaatt atgtgcgtaa
    18421 aggtttttat aatgacacga tttttcatcg tgttatcgat ggttttatga ttcaaggcgg
    18481 cggtttgaat acaaatatgg agcaaaaaac aactgcctct ccaattcaaa atgaagcaaa
    18541 aggagcgaaa cctaacaaac gtggaaccat tgcaatggcg aggacaatgg atccccattc
    18601 tgcaacagcc caatttttta ttaatgttgc ggataatggt tttctgaatt acagtggcga
    18661 ccatcctcaa ggttacggtt actgcgtatt tggggaagta gttgaaggga tggatgtggt
    18721 tgatgctatt gctaaagtga aaacaggcca acgaaatggt catgcagatg ttcctgtaga
    18781 agaaataagc atcattgaag ttagagaatt ggcttagcga gaaaatacat ggctgaaata
    18841 gcataggttt tccatggcaa aagtatattt tctttaattt catccccagt atggaagagg
    18901 gatcttcctt atacatgcag gaagccgcat ttgcaaaaca gcgtgccttt tttcaggggt
    18961 gaaatatcaa ggggtatctc taaaagatta acatcattat ttaagtgttt tagtgaaaaa
    19021 aaacgttatt tttttctcct ctttatactc tctgagtaat caaaaattgg aatttcaata
    19081 tgaaaaaaaa tagccaataa gcgtgcggta aaaatgcata acaaagtgat aatgatattt
    19141 aaattatgag ggatatgaaa atagtctaat gttataaaca gaagcgcacc agccagggca
    19201 ataacagcat ataattcttt tcttaaaacc agaggaatgt cattacataa aatatctcgc
    19261 aacattcccc cgcaaatccc ggtaatcatt ccactgatta ctgcaacact tggtggcata
    19321 ccatgggcga gaactttttg tgtgccaata ataacaaaag ttgagagacc aagggcatca
    19381 agaattaaga atactttcat tattctaacc agtgaatgag caataaaaat agttagaaaa
    19441 gcgcatactg aggtatataa aagatattcc gggtgagcca cccaggtcag tggataggtg
    19501 ttaaataatg tatcccttac ggtccctcct cccagtgcag tgattgacgc aataagtagc
    19561 accccaaaaa agtccatttt tttacgtcct gctgccaggg ctcctgtaat cgcttctaca
    19621 caaatcccca caataaaaag ataatgaagt aacatatgat ttgctgctat ttaaaaagac
    19681 aatgatagca ctaatagtac aaatgaagaa atgctgtact tcgactagaa tttaatagta
    19741 aggttttaat tattttggca ggagcaaatt gatgaataat acgatcgata atcaatcgca
    19801 agaagaatta actcattttg atgcaaatac gccccagagc atcattcaat tggatagaaa
    19861 gctcagagaa atgaaaagcg aagtcgatat gaaactaaat gatccttcat cttataatgg
    19921 ggatgataaa gaagtttatt ttcaaaaat
//
LOCUS       DBNCWK010000047        19669 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.047, whole genome shotgun sequence.
ACCESSION   DBNCWK010000047 DBNCWK010000000
VERSION     DBNCWK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19669
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.047"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..440)
                     /locus_tag="AC06IO_001489"
     CDS             complement(<1..440)
                     /locus_tag="AC06IO_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874684.1"
                     /translation="MKITRNQLNFKGVKEFGGCLTRNDEQIEFINKEIKLLKELIEFN
                     ILKGIDENKSQSLFDAVIEAVEKAGLDVEHELLVQFIQSTSEIIDSKSTDLPELVSLL
                     TVLIKSSKKYDKSELTRYCRLLDKLEKTDEKTLEQWIKILTILG"
     gene            complement(529..2337)
                     /locus_tag="AC06IO_001490"
     CDS             complement(529..2337)
                     /locus_tag="AC06IO_001490"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HIO9874685.1"
                     /translation="MQNTAKINQLSVAFKSPDKTVLALDKLSFSLNPGETLVLLGESG
                     CGKSLTSLAMMRLLPRAGVYGIDSQINIHDEDILNLPEKMMRELRGRRLAMIFQEPMT
                     ALNPVLTIGEQIAEVLKRHRFLTKEQIKERVILLLNEVEMPQPQMRIYQYPHQLSGGQ
                     KQRVVIAMALACEPDILIADEPTTALDVTIQAQILTLLKKIQKKHQMSLLLITHDLGV
                     AKTMADRICVMYAGQVVEQASVKDFFSKVRHPYVQQLLTSLPSYAKRNEKLSIIAGTV
                     PSLESMPSGCRFHTRCIYAFERCHHEEPQLQEVEQRMLRCHLYPDIKELPLLDKNKIS
                     WVPSVEKEEIVFTVDDLSVHFIHKKGFLSRKKTIFKAVDGLSFTLSRGKTLALVGESG
                     CGKTTASRALLRLLPAAGGKIRYKDSDVLSMTGSALRRYRKKVQIIFQDPFSSMNPRM
                     TVGEIIAEGMHAQGMKPSYIRKQQKFLIEQVNLPSTSLHRYPHQFSGGQRQRICIARA
                     LATEPDILICDEPTSALDVSVQAQILNLLKELQQQRGISYLFITHNMGVVSYIADDVL
                     VMKDGLVVEYGACEKVLRQPEHPYTRQLLNAVLDIE"
     gene            2471..4351
                     /gene="parE"
                     /locus_tag="AC06IO_001491"
     CDS             2471..4351
                     /gene="parE"
                     /locus_tag="AC06IO_001491"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIO9874686.1"
                     /translation="MSENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSV
                     DEVLAGFASRIIVTLHEDGSIEVEDDGRGMPVDLHPQLGLSGVEVIMTRLHAGGKFSD
                     KNYSFSGGLHGVGVSVVNALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRE
                     TGTVIRFWPNGKYFDTTKISVKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQG
                     LTDYLRQTLPEDYLPEEPFAGAFKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGG
                     THVNGLRSGLFEAMSEFCELRNLLPRGVKLTADDLWESCQYVLSIKMKEPQFAGQTKE
                     RLSSRQTSGLVSNVIKDAFAIWLNQHRNQGEAIAALAIERAQKRLKQAKQVARKRVSQ
                     GPALPGKLADCLQTDLSQAELFLVEGDSAGGSAKQARNKDYQAILPLRGKILNSWEVD
                     SSQVLASQEIHDISVAIGVDPGSSDLEGLRYGKICILADADSDGAHIATLICALFLRH
                     FKPLVKAGHVFVAMPPLYRIDAGKLIHYALDDEEKNRIVQHLSQTTKSKVNVQRFKGL
                     GEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAIMDMMLNKKRAADRKLWLETKGNLA
                     EI"
     gene            complement(4418..6535)
                     /locus_tag="AC06IO_001492"
     CDS             complement(4418..6535)
                     /locus_tag="AC06IO_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIO9874687.1"
                     /translation="MFRFLFPVSLFISSILLFSIQPMVAKAILPIYGGTPGVWTVCVL
                     FFQFILLIAYGYVWLISQIKKPAVWRLIHMVLVVLSFTALPLLFHPAARDGQPEWVIL
                     HNLLIQLGLPLLVIGASAPLLQFAYSQTTSKGASDPYYLYISSNLGSLSALLFYPWVI
                     ERFIGLTRQFYLWNFGFAIYLLLLVTVLFFTKYQPLILTEKKEVDFLPWREIAYWIFL
                     SFVPCSLMLGVTLYITTDVAATPLFWVLPLALYLLSFVFTFTATPLISQKWISRNCLF
                     FLVFTILGFIFGVSQIRVWQLVLFNLLSFFILALLCHGQLFARRPKPHKLTLFYFCLS
                     IGGVLAGIFNGILAPNWFNYVYEYPLAILLSLFALQLPKTNRGWWVPLVVLGLLSLHY
                     FIPTVPWFKSTTTFHILAILALGIIVIWHRNKINLVLSMLILFVFLYFPPLQDKQILL
                     KERNFYGVKQVFKKDDVHALISQSTLHGMQVMNGEKASYGRSSYYGAIAPIVDILKKE
                     FHPLSATIMGLGAGTLLCQFRATDSIKVIEIDQQVIDLAKNNKLFTYIRDCSPSAEII
                     KQDGRLALVNMPDASQDLLILDAFNSDAIPVHLMTLEAFTLYKKKIVEDGVILVNLSN
                     RHLQMLPVINAIGRSLDMITLFLAHKGDKKLGQFNSEWAMLTSNQSLAFQVMKGTNWK
                     FVADDNQFLWTDDYSNIIPLLKW"
     gene            complement(6792..7232)
                     /locus_tag="AC06IO_001493"
     CDS             complement(6792..7232)
                     /locus_tag="AC06IO_001493"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIO9874688.1"
                     /translation="MSKVIKKLEVALADTYALYLKTQNYHWHVTGPQFKSLHELFEMQ
                     YKELAEAVDQIAERIRIMGHKAPATFTEFNQLKTIKDGDSGLSANEMVSELAKDNMMI
                     VKDLNQAIKIAQENEDEGTVTILSDRVAAHEKAHWMLSASKEKK"
     gene            complement(7355..9004)
                     /gene="groL"
                     /locus_tag="AC06IO_001494"
     CDS             complement(7355..9004)
                     /gene="groL"
                     /locus_tag="AC06IO_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820699.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIO9874689.1"
                     /translation="MMAKELRFGDDARLQMLAGVNALADAVQVTMGPRGRNVVLEKSY
                     GAPTVTKDGVSVAKEIEFEHRFMNMGAQMVKEVASKTSDTAGDGTTTATVLARSILVE
                     GHKAVAAGMNPMDLKRGIDKAVLAVTKKLQAMSKPCKDSKAIAQVGTISANSDEAIGA
                     IIAEAMEKVGKEGVITVEDGNGLENELSVVEGMQFDRGYISPYFINNQQNMSCELEHP
                     FILLVDKKVSSIREMLSVLEGVAKSGRPLLIIAEDVEGEALATLVVNNMRGIVKVCAV
                     KAPGFGDRRKAMLQDIAILTKGQVISEEIGKSLEGATLEDLGSAKRIVVTKENTTIID
                     GEGKATEINARITQIRAQMEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALIRAQKALDSLKGDNDDQNMGINILRRAIESP
                     MRQIVTNAGYEASVVVNKVAEHKDNYGFNAATGEYGDMVEMGILDPTKVTRMALQNAA
                     SVASLMLTTECMVADLPKKEEGVGAGDMGGMGGMGGMGGMM"
     gene            complement(9029..9319)
                     /gene="groES"
                     /locus_tag="AC06IO_001495"
     CDS             complement(9029..9319)
                     /gene="groES"
                     /locus_tag="AC06IO_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820700.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIO9874690.1"
                     /translation="MKIRPLHDRVVVRRMEEERTTAGGIVIPDSATEKPMRGEIIAVG
                     AGKVLENGDVRALAVKVGDVVLFGKYSGTEVKVDGKELVVMREDDIMGVIEK"
     gene            9505..11265
                     /gene="dsbD"
                     /locus_tag="AC06IO_001496"
     CDS             9505..11265
                     /gene="dsbD"
                     /locus_tag="AC06IO_001496"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIO9874691.1"
                     /translation="MKKWLLFSLLFLTSLCVHAEPLPASEVFKFSVKKIDPNTFAIQW
                     NILPKYFLYSDRIQLNSDNDDIAQLGTLRFPTPLTKTDKQGRTFKIYRNQLNLPVGVL
                     GITPGETIVNLRFQGCADDGFCYPPEVKQIKLAIDDKLALSQVDLETFHAPEATPLEK
                     PQQDIADIFANHNWIMILLIFYGFGLLLSFTPCILPMVPVLSGIIVGHGKTATTKKAF
                     FLSLSYVLSMSVTYAVVGAVVALLGANLQISMQSPWAISLFSLIFVLLALSMFGFYEF
                     KLPDAWQSKIVGSSREQRGGHYLGAAIMGCLSTLILSPCVTAPLIGVLTYIAQTGNVL
                     LGSVTLFTLSLGMGTPLLLIGTSAGKWLPETGSWMNAVKAFFGILLLAVAIYLMARIL
                     PAGLVMGLWACLLIFSGIYSGALTKSNTNQEKLCQGIGIILLTYGLLILIGASMGSSN
                     PLQPLANLQAAPTVSNAFQSAKAQSVKSVELAIKQAFGKPVMLDFYADWCASCKVMEN
                     TTFKDPRVQKALSHFIVIKVDVTANNKNDRALMQHFRVVAPPTFIFFNAQGIQLNNLK
                     RVGEFNADEFMQTIKNIDAN"
     gene            11355..12662
                     /gene="rimO"
                     /locus_tag="AC06IO_001497"
     CDS             11355..12662
                     /gene="rimO"
                     /locus_tag="AC06IO_001497"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIO9874692.1"
                     /translation="MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVV
                     INTCGFIDSAVQESLDTIKEAMAENGRVIVTGCLGAKADVIKNACPDVLHISGAHAYE
                     EVVNAVHQHLPPPADPFTQLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKL
                     QSYPMAQILTEAKKLKQAGVKELLVISQDTSAYGVDTRYQPVEWQGKTVNTRFYDLCE
                     QLGELGIWVRLHYVYPYPHVDDIVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSE
                     NTLLRIASWREICPDITLRSTFIVGFPGETEEEFSELLAFLKEAQLDRVGCFKYSPVE
                     GAKANDLDNPVSEDIKEERYHRFMQVQAEISRNKLKNKIGSTQTVLIDEITEDQIIAR
                     SKSDAPEIDGLVYLPKISGITVGSFAEVVITDSDDYDLYASLV"
     gene            complement(12763..13899)
                     /locus_tag="AC06IO_001498"
     CDS             complement(12763..13899)
                     /locus_tag="AC06IO_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9874693.1"
                     /translation="MKSHFSMQRLWAIIRKEFLLMKRDPATIVIMAILPLILVCIAGY
                     AINTYPQKVPTVLINLDNTDVTRELVREIQNTGYFSFVASTNDSESAHHLLKTGKAIL
                     VLTIPVNFTKAFRRNQNPALLLEDGSIDAIASGRAIIALAGLKSSFIEKMGQKGINIP
                     LQRPDFQIINHRLYDPDRVTQFYVVPGMIGLVLMLTMLLITVVVAFRDVQGGTIEYLL
                     ASPTRPSEILLGEIFSYIVIGYIQLSFGLILSYYLFHVPFIGAPLLLYLCTLPYIVAE
                     LSLGLTIATFCTSQFEAVQAINVFIAFSILLTGFTFPIFGMPEWAQYLGLFLPLTHFF
                     KIMFGIMLKGNNFGEIWANLWPLLAYCLTMISLATWRFKRHFVR"
     gene            complement(13896..14822)
                     /locus_tag="AC06IO_001499"
     CDS             complement(13896..14822)
                     /locus_tag="AC06IO_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9874694.1"
                     /translation="MSKWSSKQIIIDVSGLAKSFNGVNAVVNLNLQIQEGTIFGFLGP
                     NGSGKTTSLRLLSGLIIPDKGQGRCLNLDLMTQTKLIQAQIGYMPQNFCLYQNLTVYE
                     NLDFIARIYQLKNRKERLKEIIELLALSDNQKRITATLSGGWKQRVSLAAALLHKPRI
                     LLLDEPTSGIDPQSRLLIWDHIQSIVSKGVTVLLSTQHMDEAERCHQLVYMSTGEIIA
                     RGSVIEIIQSTGLHSWVVKGESFSRLKDILVNNPAIQMIEKGNEMRISSIVDDIHQVL
                     DPFVLNNYEIKKADTTLEDAFIYKIMKEGNPK"
     gene            complement(14815..15387)
                     /locus_tag="AC06IO_001500"
     CDS             complement(14815..15387)
                     /locus_tag="AC06IO_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIO9874695.1"
                     /translation="MKIGIAILLVLVCCTLQCCKKEKSPYQTTSYVESQLFIVSSPDG
                     GYIKEWYADEGQLLHKDDKIVTLDGVNSIKAPADSVMTERYYLKDEYVPPNFPIASLS
                     LPSQMKILFYVPESHLEKIKLGKKIRILLNEKKYSGKISFISNQAEYTPDVIFSEKNR
                     YKLVYKVKADLSQELRDLLKIGQPVEVNYE"
     gene            complement(15455..16666)
                     /locus_tag="AC06IO_001501"
     CDS             complement(15455..16666)
                     /locus_tag="AC06IO_001501"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIO9874696.1"
                     /translation="MHANYYDMYQKTGIFSDEFFGQFLRTLGKCYVRCADVGECYTTL
                     SKITDKDGESWYKAWYEMADILKTKANISWEANHFITAAYTYLRAAEYYRASEFFLRA
                     NPDDPRVIPCFDQLQFCFERAMHRLHPGSQKIWIPYDDQTLSGYLFFSDQSPKATLIV
                     PGGYDSTFEENYALVPAALLHGYHILIFDGPGQGHVLRRQKLYMRADFEHVVSQVLDW
                     LDEQNGLGTSNYVLIGRSFGGYLAPRAACSEKRLAALICDPGQMDIAGSLNKMLPPNL
                     MMLLKEGKADELNACFYQLFSENPMKEFYFKSRMYTHGLTTPFDYLQALSAYTFADRV
                     QEITCPTLVCDNPSDRISNRGNTLYEALRCEKTYVMFDAKLGAGMHCEADANGQFQQV
                     IFDWLDERFTQ"
     gene            complement(16935..>19669)
                     /gene="glnE"
                     /locus_tag="AC06IO_001502"
     CDS             complement(16935..>19669)
                     /gene="glnE"
                     /locus_tag="AC06IO_001502"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIO9874697.1"
                     /translation="QSDIETLISTKSWLIEKYVAPLHHPLRESAEKLILVSDYASRQI
                     KLLVSLLESDSCCSLWPRESYFYAIQQISLELSQAQYMQELRRFRHTHFLRLLLLEIT
                     GLASTQEVMQSWSDLADSIILHTLKYIGFNLLSRYGVPRNSEGKEIQLYALAMGKLGG
                     RELNYSSDIDLIFAFNEVGSTDGEEQITNQHYFSKMVQTFVHLLQSVTPDGFVFRVDL
                     RLRPNGDSGPLVSSFAAMETYYQEQGRDWERYAMVKARVIAESLNEPFPWFERLIIPF
                     VYRRYVDFGVIESLRGMKAMIEREVQLNPLLNDIKRGRGGIREIEFIIQNIQLIRGGR
                     LPQLQVQNAMLALAVIKKEKLLPRCDALKQAYLFLRKLENILQSLNDQQTHSLPEDQL
                     KKVQIVYAMGFTDWEQFFNKLQQYQRIVSNFFHSILGKVDDYEDEKRLLNNQLFSLWQ
                     GHVESNMAVNLLISLGYENAQHCYQMLHAFRHGPRCRRLSQGARIRLDRFMVLLLTEL
                     TYCPKTDEVLLQVIHLLENIVGRSAYLALLTENPHALRELLFWFVNSPFISSLFVSQP
                     FLLEILLEQEKDWHPDSLSQMEQTIAEKLEHTNDVEVHEEILRQFKLTNWMVAARAEL
                     YGLCDAVRIGKFLSDVAQVIVSQVLIIASKQLCGRHPEMAQIQSRFAIIAYGKLGSRE
                     MSYASDLDLVFLHSAKPSEEALVTRLTQKILHMLTTRTQMGVLYHVDTRLRPSGAAGL
                     LVSHVDAFVEYQKTQAWTWEHQALLKARILSGNLKIKNQFSQLKKSVLLLERDSKLLQ
                     RDVLTMRSKIDQFQESDEVKLARGGLLDLEFLVQFLILNLRDPKFSSYTNTLSQLKHL
                     FLSNVLTKSQFTTLKKAYQYFHYLMHQKIVQSDFINDDEMQNAVSSVCKDLYRY"
BASE COUNT         6188 a         4157 c         3528 g         5796 t
ORIGIN      
        1 cttccaagta tagtcagtat ttttatccat tgttccaagg ttttctcatc tgttttttca
       61 agcttatcga gaagacggca atatctggtc aattctgatt tgtcatactt cttacttgat
      121 ttaatgagta ccgttaataa ggaaaccaac tcaggaaggt ctgtcgattt ggagtcaata
      181 atctctgaag tggattgaat aaattgtacg agcagttcat gctcgacatc caagccagcc
      241 ttttccactg cctcaatcac agcatcaaat aaggattgtg atttgttttc atcgattccc
      301 ttcaggatat tgaattcgat taattccttc aacagcttga tctctttatt aatgaattca
      361 atttgttcat catttcttgt taaacaacca ccaaattcct ttacaccttt gaaatttaat
      421 tgatttcgag tgattttcat gccctgaatt ccctgaaaga tagaatgccc tcattatata
      481 taagctgcat aaaatgtagc agataaatgt gaaagaaaat ttgaaatatt actcgatgtc
      541 caatacagca tttaatagct gcctggtata agggtgttct ggttgccgaa gtaccttctc
      601 gcacgctcca tattcaacaa ccaagccgtc tttcatgact aatacatcat cagcgatata
      661 agagacgacc cccatgttat gggttataaa caaataggaa atacctcttt gctgttgcaa
      721 ttcctttaat aaattaagaa tttgtgcctg aacagagaca tcaagagcac tggtcggttc
      781 atcgcagatt aaaatatcgg gttctgtcgc caaagctctc gcgatgcaga tccgttgtct
      841 ttgtccaccg gaaaactgat gggggtatcg atgcagactg gtacttggta agttcacttg
      901 ctcaattagg aatttttgtt gtttcctgat gtaagagggc ttcattccct gtgcatgcat
      961 cccttctgcg atgatctctc caactgtcat gcgtggattc atagaagaaa aaggatcttg
     1021 aaaaataatc tgtacttttt ttctgtatct tcttaaagcg cttcctgtca tagacagtac
     1081 atcactatct ttatatctga ttttcccccc ggccgctggc aataacctca acagagcacg
     1141 gcttgcagtt gttttcccac agcccgactc tccaaccaaa gctaatgttt tacctctgga
     1201 gagagtgaac gacaaaccat caacggcttt gaaaatggtt tttttacggc tcaaaaatcc
     1261 tttcttatgt ataaaatgaa ctgataaatc atctactgtg aacacgatct cttctttttc
     1321 aaccgaagga acccaactga ttttattttt atccagtaat ggcaattctt tgatatcagg
     1381 atacaaatga catctcaaca tacgctgttc aacttcttgt aattgaggct cctcatggtg
     1441 acaacgttca aaagcataaa tacagcgtgt atggaaacga caccctgaag gcatagattc
     1501 caaagaaggt accgtacctg caatgataga aagtttttca tttctttttg catatgaagg
     1561 tagggatgtc aaaagttgct ggacataggg atgtctcact tttgaaaaaa aatcttttac
     1621 tgaggcctgt tccactacct gccctgcata catcacacaa attctatcag ccatggtttt
     1681 agccacaccc agatcatgag taattaataa cagactcatc tgatgtttct tttgtatttt
     1741 ctttagtaag gtcaatattt gagcctggat agtcacatcc aaagccgtag taggttcatc
     1801 ggcaataagg atatctggct cgcaagccaa cgccatagca ataaccaccc tctgcttttg
     1861 accaccagac aattgatgtg ggtattgata aattctcatt tgtggttgag gcatttctac
     1921 ctcattcaat aacaatatta ctctttcttt tatttgctct tttgttaaaa accgatgcct
     1981 ttttaaaacc tcggctatct gctctccaat cgttaataca ggatttaaag cagtcatcgg
     2041 ctcttgaaaa atcatcgcca gcctgcgccc tcttaactcc ctcatcattt tctcaggtaa
     2101 atttaagatg tcttcatcgt gaatattgat ttggctgtca atcccataaa caccagccct
     2161 gggcagcaat ctcatcatag ccaatgaagt taatgattta ccacatcctg actctcccag
     2221 taaaaccaaa gtctcccctg gatttaggct aaaacttaat ttatccaaag ccagaactgt
     2281 tttatcaggg cttttaaaag caactgacaa ttgattaatt ttagcggtat tttgcatgga
     2341 atttgccaca aataatgata actacactct aacaagagag aggcgatagt agcaataaaa
     2401 ataccatacc actctgagca aaagaatgtt tatgatagaa taagcacaat taatatttga
     2461 gtgaataccc atgtcagaaa actacactgc ggaagcgatt gaagtcttaa gtggtcttga
     2521 gcctgttcaa cgacgtcccg gtatgtatac cgacacgaca cgccccaacc atttggctca
     2581 ggaagtgatt gataatagcg tcgatgaagt acttgctgga tttgccagcc gtattattgt
     2641 tactttacat gaagacggtt ctattgaagt cgaggatgat ggccgaggta tgccagttga
     2701 tttgcatcct caactcggct taagtggtgt ggaggtcatt atgactcgac ttcatgctgg
     2761 aggtaaattt tcagataaaa attacagctt ttctggcggc ttgcatggcg ttggggtgtc
     2821 cgtggtaaat gccttgtcag aacgacttga cgtcaccata aagcgcaatg ggatcattta
     2881 tcaaatgacc tttgctaatg gcgacaaatt atgtgaatta aatgaaaccg gggttacaaa
     2941 aaaaagagag accgggactg ttatacgttt ttggcctaat ggcaaatatt ttgatactac
     3001 aaaaatttct gtaaaacacc tgactcatgt attaagagca aaagcggtat tgtgtagcgg
     3061 cttgtccatg acctttatca ataaaatcac taatgaagaa atgaactggt gttatgaaca
     3121 aggtttaact gattatttac ggcaaacctt gcccgaagac tatttgcctg aagaaccttt
     3181 tgccggagca tttaaaagtg atgaagcaac cgttgattgg gctttggcct ggtcagactc
     3241 tgccaatggc aatttaaatg aaagttatgt gaatttaatc ccgacaatac aaggaggtac
     3301 gcacgtcaat ggattgcgtt cgggattatt tgaagccatg tctgaatttt gtgagctgag
     3361 aaaccttctt cctcgtggag taaaattgac agcggatgat ctatgggaat catgtcagta
     3421 tgttttatca atcaaaatga aagagccaca atttgctggg caaaccaaag agcgcttaag
     3481 ctcacgccaa acctcaggac tagtcagtaa tgtcataaaa gacgcttttg caatttggtt
     3541 gaatcagcat cgcaaccagg gtgaagccat agcagctctt gcaatagaac gcgcacagaa
     3601 acggctaaag caagccaagc aagtggcacg caaacgtgtt agccaagggc ctgctttacc
     3661 tggaaaatta gccgactgct tgcaaacgga cttaagtcaa gcggaactat tcctggttga
     3721 aggagattcg gcaggagggt cggccaagca agcccgcaat aaagattatc aagccatttt
     3781 acccttacgt gggaaaatac ttaattcatg ggaagtagat tcttcacaag ttctagcatc
     3841 ccaggaaatt catgatatat ctgttgctat aggcgtagat cctggctcta gcgatcttga
     3901 gggtcttcgc tatggaaaaa tctgtattct tgccgatgca gactctgatg gagctcatat
     3961 tgccacacta atatgtgcct tgtttttaag acacttcaaa cctctggtta aagcaggcca
     4021 tgtttttgta gccatgcccc cgctctatcg aatcgatgca ggtaagctga ttcactatgc
     4081 cctggatgat gaagaaaaaa atagaatcgt tcaacatctc agtcaaacca caaaatcaaa
     4141 agtgaatgta cagcgattca aaggattagg tgaaatgaat cccattcagc ttagagaaac
     4201 aacaatggat cccaatacaa ggcgtctggt acagcttact cttgatgatg aagagcagac
     4261 ggaagccatt atggatatga tgctcaacaa aaaaagggcc gccgacagaa aactctggct
     4321 agaaacaaaa ggaaaccttg ctgaaatttg agctatggat agcccgcatc acgataaagc
     4381 aaggatacaa ttgaatttga tagcttatct ttttacccta ccatttcagc aagggaataa
     4441 tattagaata atcatcggtc cataaaaact gattatcatc tgctacaaat ttccagtttg
     4501 ttcctttcat gacttgaaac gccaaggatt gattcgaggt cagcatagcc cattctgagt
     4561 taaattgccc caatttctta tctcccttat gcgctaaaaa taaagttatc atatccaaag
     4621 aacgtccaat tgcattaatt accgggagca tttgcagatg tctgttactt agattaacca
     4681 aaataactcc atcttcgaca attttcttct tatataaggt gaaagcctca agtgtcatca
     4741 gatggacagg aatagcatct gaattaaacg catccaaaat taacaaatcc tgtgaagcat
     4801 ctggcatatt aaccaaggcc aatctaccat cctgtttaat aatctcagct gaaggcgaac
     4861 agtctcgtat gtatgtgaat aatttattat ttttagctaa atcaattact tgttgatcaa
     4921 tctcaataac ctttatggaa tcagttgccc gaaattggca cagtagtgtt cctgcaccca
     4981 accccataat cgtcgcagaa agcggatgaa attccttctt caaaatatca actattggag
     5041 ctatggcccc ataataggag cttcttccat aacttgcttt ctccccattc attacctgca
     5101 tcccatgaag ggttgattgg cttattaatg catgcacatc atcctttttg aagacttgtt
     5161 tgacgccgta aaaatttctt tccttgagta aaatctgttt gtcttgcaac ggaggaaagt
     5221 aaaggaaaac aaataaaatg agcatggata gtacaagatt gattttatta cgatgccaaa
     5281 tcacaataat tccaagtgcc aaaatagcaa ggatgtgaaa agtggtagta cttttaaacc
     5341 aaggaacggt aggaatgaaa taatgaagcg acagcaaacc caaaacaact aatggcaccc
     5401 accatcctcg gttagttttc ggcagctgaa gagcaaataa acttaataaa atggctaatg
     5461 ggtattcata aacataatta aaccaatttg gcgctaaaat tccattaaaa atccctgcta
     5521 aaactcctcc gatagacaaa caaaaataaa acaaagtgag tttatgtggc ttgggtcttc
     5581 gcgcaaataa ctggccatga cacagtaaag ccaaaataaa aaaacttaac agattaaata
     5641 acaccaattg ccacactctg atttggctaa ccccaaaaat gaacccaaga attgtaaaaa
     5701 ccaggaaaaa caggcaatta cgagaaatcc atttttgtga aataaggggg gtcgctgtga
     5761 aggtaaatac aaatgacaaa aggtaaagcg ctaaaggcaa aacccaaaat aagggagttg
     5821 cagcaacatc agttgtgata tataaagtaa ctcccagcat caaactgcaa gggacaaaac
     5881 tcaaaaaaat ccaataggct atctctcgcc aaggcaaaaa atccacctct tttttttcag
     5941 ttaatattaa aggttggtat tttgtaaaaa acaagactgt taccaacagt aacaaataaa
     6001 tagcaaatcc aaagttccat aaataaaatt gacgagtcaa accaataaat cgctcgataa
     6061 cccatggata aaaaagcaag gcactcaagc ttccaagatt actggatatg tacaaataat
     6121 aaggatctga ggcccctttg cttgttgtct ggctataggc aaattgcaat aaaggagcag
     6181 aagcaccaat tactaacaaa ggcaaaccca gttgaattaa caaattatgc agaatcaccc
     6241 attcaggctg tccatccctg gctgcaggat gaaacagcaa tggaagtgct gtaaaactta
     6301 aaacaaccaa aaccatatgg atcaaacgcc aaacagcagg tttcttaatc tgactaataa
     6361 gccatacgta gccatatgca atgagcagaa taaattgaaa aaaaagcacg caaacagtcc
     6421 acacacccgg tgtccctcca taaattggaa gtattgcttt tgcaaccatg ggttgaatac
     6481 tgaataggag aatggaactt ataaaaagac tgacaggaaa taaaaatcga aacacaaagc
     6541 aatccttagc caatggtgct gccaaatgaa tgaatcaatc attttacgac tacactctcg
     6601 ttcatacaag gatgaattat tactctgcaa aacaatttgt aaacataatt gatagggatg
     6661 tttcacaagc acatattatt ccatattgac agatggaata atccatttat ggtttttgaa
     6721 gcttttgtaa acatcccatt acttgaagat aggcactgat tccatatcaa ctgacacata
     6781 aaatggatac tttatttctt ttcctttgaa gctgataaca tccaatgagc tttttcatga
     6841 gccgcaactc gatcgcttag aatagtcaca gtaccctcgt cttcattttc ctgggcaatt
     6901 ttaatggcct gattcaaatc ttttactatc atcatattat cttttgccaa ctcactgacc
     6961 atttcattag cactcaatcc ggaatcacca tctttaatcg ttttcaattg attaaattcg
     7021 gtaaaggtag caggagcctt atgtcccatg attcgaattc gctctgcaat ttgatccact
     7081 gcttccgcca gttctttata ttgcatctca aataattcat gcaaactttt aaattgaggt
     7141 ccggtaacat gccaatggta attttgtgtt ttcaggtaca atgcataagt atcagcaaga
     7201 gcgacttcca gctttttaat tactttactc attattcttt ccttgttatt tattcacgtt
     7261 attaaggata gttcaaaaca gtcaaagaac aaaatagaca ttaataaaaa acccgccttt
     7321 acagcgggtt ttttattgga taaccgggag aaaattacat cattccgccc atgccaccca
     7381 ttccgcccat gccgcccata tcgccggcac caacaccttc ttctttctta ggcagatcag
     7441 caaccataca ttcagtagtc aacatcaaac tggctacaga agctgcattt tgcagagcca
     7501 tacgggttac tttggttgga tcaagaatac ccatctcaac catatcaccg tattcaccag
     7561 ttgcagcgtt gaaaccgtag ttgtctttgt gctcagctac cttgtttact acaacagaag
     7621 cttcatatcc tgcgttagta acaatctgac gcattggaga ttcaatagcg cgacgtaaaa
     7681 tattgatacc catattttga tcgtcattat cgcctttcaa tgagtcaaga gctttctgag
     7741 cacgaatcaa ggcaacacca ccaccggcaa cgataccttc ttctactgca gcgcgagtag
     7801 catgaagagc atcttcaaca cgagctttct tctctttcat ttcaacttct gtggcagcgc
     7861 caactttgat aacagcaaca ccaccagcta acttagcaac gcgctcttgt aatttttctc
     7921 tatcgtaatc agaagtggtt tcttccattt gtgcacgaat ttgagtaata cgagcattaa
     7981 tttcagttgc ctttccttca ccatcaatga tagtggtgtt ttctttggtc acaacgattc
     8041 gcttagcact accaagatct tccagagtag caccttccag gctcttgcca atttcttcag
     8101 aaataacttg gcctttagtc aaaatagcaa tgtcttgcag catcgctttg cgacgatcac
     8161 caaaaccagg cgctttgaca gcacatactt ttacaatacc gcgcatgttg ttaactacca
     8221 gagtagctaa agcttcgcct tcaacatctt ctgcgataat caataaagga cgtccagatt
     8281 tggcaacacc ttccaatacg gacaacattt cacgaatact ggaaactttt ttgtcaacca
     8341 ataaaatgaa tggatgctca agttcacagc tcatgttttg ctggttgttg atgaagtatg
     8401 gagaaatgta accgcgatca aattgcatac cttcaacaac agaaagctca ttttccaatc
     8461 cattaccatc ttcaacagta ataacaccct ctttaccaac tttttccatt gcttcagcaa
     8521 tgatagcacc aatcgcttca tcggaattag cagaaatagt tccaacttga gcaatagctt
     8581 tgctgtcttt gcatggctta gacatagctt gtaatttttt ggtaactgcc aatactgctt
     8641 tatcaatacc gcgtttgaga tccattggat tcataccggc agcaactgct ttgtgacctt
     8701 caacaagaat agaacgagcc aatactgttg cagtagtagt accatcacca gcagtatcag
     8761 aagttttaga agccacttct ttaaccattt gagcgcccat gttcatgaaa cgatgctcaa
     8821 attcaatttc tttggcaaca gacacaccgt ctttagttac agtaggagcg ccataagatt
     8881 tttctaatac aacattacgg ccacgtggac ccatagtaac ttgaaccgca tctgctaatg
     8941 cattaacacc agcaagcatt tgtaggcgag cgtcatcacc aaaacgtaat tctttagcca
     9001 ttatcattgt ctcctttaaa caattagatt acttctcgat tacacccatg atgtcgtctt
     9061 cgcgcatcac aactaattct tttccatcaa ctttaacttc agtaccagag tacttgccaa
     9121 acaatacaac atcaccaact ttaactgcta aagcacgaac atcaccgttt tccaatactt
     9181 tgccagcgcc aacagcaatg atctcaccac gcataggttt ctcggtagca ctatctggaa
     9241 taacaatacc accagcagtg gtacgctctt cttccatacg acgaacaaca acgcgatcgt
     9301 gtaaaggacg aattttcata cttttctctc ctgattaaat taaactttaa atcatttaag
     9361 tattgctttc cttagggcaa acaataccga tgacagcgat atggggacaa taaacaaact
     9421 ttcaagggta aaatcaaaaa aaattttgga tttgggatta cagagtctac aataatgttt
     9481 attagtaaca gtaaaagata tcgaatgaaa aaatggctct tattttccct attattttta
     9541 acttccctct gtgttcatgc tgagcccttg ccagcttctg aagtatttaa gtttagtgta
     9601 aaaaaaatag accccaatac ctttgccata caatggaata tcttgcccaa gtatttttta
     9661 tatagcgacc gtattcaatt aaattccgat aatgatgaca ttgcacagct tggaactctt
     9721 cgcttcccca cccccttaac caaaacggat aaacaagggc gaacctttaa aatttatcgc
     9781 aatcagctta accttccagt gggagtcctg ggcattaccc caggagaaac aatagtcaat
     9841 ttgcgttttc aaggatgtgc cgacgatggt ttttgttatc cccccgaagt aaaacaaatc
     9901 aaactggcaa ttgacgataa actggctctt tcccaagttg atttggaaac atttcatgca
     9961 cctgaagcaa caccccttga aaaaccgcag caagacatcg ctgatatttt tgcaaatcat
    10021 aattggataa tgattctgct tatattttac gggtttggcc tgctcctgtc cttcactcct
    10081 tgtatacttc ccatggttcc tgtcctttca ggcattattg ttggtcatgg gaaaactgca
    10141 accacgaaaa aagcattttt cctgtcatta agctacgtgc tcagcatgtc tgtcacttat
    10201 gccgttgtgg gtgctgtcgt tgccctgctt ggagccaatc tacaaatcag tatgcagtca
    10261 ccatgggcta tcagtctatt cagtctgata ttcgttttgc tagccttgtc catgtttggc
    10321 ttttatgaat tcaaactccc tgatgcgtgg caaagcaaaa tagtgggttc aagtcgtgaa
    10381 caacgaggcg ggcactatct tggtgcagcc atcatgggat gtttgtccac tctcattcta
    10441 tcaccatgcg tcacagcacc attaatcggg gttttaactt acatagcaca aactggaaat
    10501 gtattactcg gcagtgtcac tctatttacc ttaagtcttg gcatgggcac tccattgctg
    10561 ctgataggaa cgtctgccgg taaatggcta cctgaaacag gaagctggat gaatgctgtt
    10621 aaagcttttt ttggcatatt acttcttgct gttgctattt atctgatggc acgcatttta
    10681 ccagcaggtt tggtcatggg gctatgggct tgtctcctga tcttttccgg catttactct
    10741 ggtgccttaa ccaaatcgaa taccaaccag gaaaagctct gccaaggcat tggtatcatt
    10801 ttattaacct atggattgct gattctcatt ggagccagca tgggctcttc caaccctttg
    10861 cagcctttag ccaacttaca agcagcccct actgtaagca atgcttttca atcggctaaa
    10921 gcccaatctg ttaaaagtgt tgaactggct ataaaacaag cgttcggaaa accagtgatg
    10981 cttgattttt atgctgactg gtgtgcatcg tgtaaagtaa tggaaaacac cacgttcaaa
    11041 gatcctcgtg tacaaaaagc tttgagccat tttatcgtta tcaaagtgga tgtgactgcc
    11101 aataataaaa atgacagagc attaatgcag catttccgtg tagtagcccc cccgactttt
    11161 atctttttta atgctcaagg catacaactc aataatttga aaagagttgg tgagtttaac
    11221 gccgatgaat tcatgcaaac aataaaaaac attgatgcga actaaagtaa ctatgggcac
    11281 ttttgtatga aatcagtcga cactggaacg attaagcagt ataattagct catttttttt
    11341 acagatagaa gtttatgaat cataaagtag gatttgtaag tttaggatgc cccaaagcac
    11401 tggtcgattc agagcgcatc attactcagc ttaaagcaca aggctatgaa ttggttccca
    11461 cttatgagga tgctggagtc gtcgtaatta atacgtgcgg cttcattgac agcgctgtcc
    11521 aggaatcact ggatacgatt aaagaagcta tggctgaaaa cgggcgagtg atcgttactg
    11581 gatgtttggg agctaaagcg gatgtgatta aaaatgcctg tcctgatgtc ctgcatatca
    11641 gtggcgcgca tgcctacgaa gaagtagtca atgcggtaca ccaacacttg cctcctcctg
    11701 cggacccctt tacacaactg attcccccac agggaataaa actcacacct aggcattatg
    11761 cttatttaaa aatatcagaa ggatgcaacc aaaaatgcac tttttgcatt atccctacga
    11821 tgagaggaaa attgcaaagc tatccaatgg cccaaattct tactgaagct aaaaaattaa
    11881 aacaagccgg agttaaagaa ttactggtta tctcacaaga caccagtgct tatggcgtgg
    11941 acacacgtta tcagcctgta gaatggcaag gaaaaacagt caatactcgt ttttacgatc
    12001 tatgcgaaca attaggtgaa ttgggaattt gggtgagact ccattatgtc tatccctacc
    12061 cccatgttga tgatattgtt cctctcatgc gcgatggcct gattttgcct tacctggata
    12121 taccattgca acatgccaat tcacgtatat taaaagcgat gaaaagacct gccagtagtg
    12181 aaaatacctt attgcgcatt gcttcatgga gagaaatttg ccctgacatt acgttacgct
    12241 ctacattcat tgtaggtttc cctggtgaaa cagaagagga gttttccgaa ttactagcct
    12301 tccttaaaga ggcacaacta gacagagtcg gttgtttcaa atactcccct gttgagggag
    12361 ccaaagcaaa tgatttggat aatcctgttt cggaagatat taaagaagag cgttatcatc
    12421 gttttatgca agtacaagca gaaattagcc gtaataaatt aaaaaataaa attggcagta
    12481 ctcaaaccgt tttaattgat gagataaccg aagatcaaat catagctcgt agtaaaagcg
    12541 atgctcctga aatcgatggt cttgtttatt tgcctaaaat atccgggatt actgtaggat
    12601 ctttcgctga ggttgttatc actgacagcg acgattatga tttatatgct tctttagtct
    12661 gaggaaattg cattaaatta aaatttttat gtctgaaaag tccgcttcct cacagtcgga
    12721 ttatgaatca accatttgtc aatatgattc ttgatgtatc ttctatcgca caaaatgcct
    12781 tttaaatcgc catgtcgcta aagaaatcat ggtaaggcaa tacgcgagta gtggccataa
    12841 attagcccaa atttcaccaa aattatttcc ttttaacata atgccgaaca taatcttaaa
    12901 aaaatgcgtc aatggcaaaa ataatcctaa atattgcgcc cattcaggca ttccaaatat
    12961 cggaaaagta aatccagtta acaaaataga aaaagcaata aaaacattga tcgcttgcac
    13021 tgcttcaaac tgggatgtac aaaaagtagc tatagttaaa cccaacgata actccgccac
    13081 aatataaggt aaggtgcata aatataaaag aagaggggcg ccaataaacg gaacgtggaa
    13141 taagtagtaa gacaagataa gaccaaaact taattgtata tagccaatga caatataaga
    13201 aaaaatttca cctaataaaa tttctgatgg acgggtcggt gaagctaata aatattcaat
    13261 cgtaccgccc tgaacgtctc taaatgccac aaccacggtt atcagcagca tcgttaacat
    13321 gagcaccaag cctatcatcc cgggaaccac ataaaattga gttacgcgat ccggatcata
    13381 taatcggtgg tttatgattt gaaaatcagg cctctgcaag ggaatattaa tacccttttg
    13441 gcccattttc tctataaatg atgattttaa tcccgccaaa gcaatgatag ctctacctga
    13501 agcaatagca tcgatactgc catcttccaa taaaagagct ggattttggt ttcgacgaaa
    13561 tgctttggta aagttcacag gaatagtcaa taccaggata gccttgcctg tcttcaataa
    13621 atgatgggct gattctgaat cgttcgtcga agccacaaat gaaaaatacc ctgtattttg
    13681 tatctctcga accagttcac gtgttacatc agtattatcc aaattgatta acaccgtagg
    13741 aactttttga ggataggtgt ttatggcata cccggctata caaactaaaa tcaaaggtaa
    13801 gattgccata atcacgatag tagcaggatc acgtttcatc aataggaatt ctttgcggat
    13861 gatagcccac aaacgttgca ttgaaaaatg actcttcact ttggattccc ttctttcata
    13921 attttataaa tgaaagcgtc ctctaaagta gtatctgctt ttttgatttc ataattgttc
    13981 aacacgaaag gatccaatac ttgatgtata tcatcaacta ttgagctgat gcgcatttca
    14041 ttaccctttt caatcatttg aatagcgggg ttatttacta atatgtcctt taatctggag
    14101 aaactctcac ctttaacaac ccaggagtgc aagcctgtcg attgaataat ttcaattact
    14161 gaaccacgtg ctatgatttc tccggtggac atataaacca attgatggca tctttctgcc
    14221 tcatccatat gctgtgtgct gagtaaaaca gtaactcctt tggatactat actttgtata
    14281 tgatcccaaa ttaacaaacg cgattgagga tcaatgcctg aagttggctc atctaacaaa
    14341 agtatacgag gcttatgcaa caaagcagcc gctaaagaca cccgctgctt ccaacctcct
    14401 gataaagtag cagtaatccg tttttggttg tcacttaagg ccagtaattc aatgatttcc
    14461 ttcagtctct ctttcctgtt ttttaattga tatattcttg cgataaaatc cagattttca
    14521 taaacagtca gattttgata gaggcaaaaa ttttgtggca tatagccaat ttgagcctgt
    14581 atcaacttgg tttgggtcat taaatcgaga ttcagacatc taccctggcc tttatcggga
    14641 attattaatc cacttaataa tcgtaaactg gtcgttttac cactaccgtt tggccctaaa
    14701 aaaccaaaaa ttgttccttc ctggatttgt aaattcaaat tcactactgc gttgacacca
    14761 ttaaatgatt ttgctaatcc ggacacatcg ataataattt gctttgagct ccatttactc
    14821 ataattgact tccacaggct gacctatttt taataaatcc cttaattcct gagatagatc
    14881 cgcctttact ttgtatacta atttataacg atttttttca ctaaaaataa catcaggagt
    14941 atattcagcc tgattggata taaaacttat tttgcctgaa tatttttttt cattgagcaa
    15001 aattcgaatt tttttaccta atttaatttt ttccaaatgg ctttcgggaa cataaaacag
    15061 gattttcatt tgagaaggca gagagagact cgcaatagga aaattaggag gtacgtactc
    15121 atcctttaaa taataacgtt cagtcataac agagtctgca ggcgctttga ttgaatttac
    15181 accatcaaga gttacaattt tatcgtcttt atgcagtaat tgaccttcat cagcatacca
    15241 ttcttttatg tatccaccgt ccgggcttga cacaataaat aactgagatt caacatagct
    15301 tgtcgtttga tacggtgatt tttctttttt acaacattgc aatgtgcaac aaactaatac
    15361 aagcaggatt gcaatcccta ttttcatacc tcttatcctt tagtcatcag tattagttat
    15421 cctatcggca aagcaataac gattgggata tccactattg agtaaatcgc tcatccagcc
    15481 aatcaaaaat aacttgctga aactgaccat tagcatctgc ttcacaatgc atacctgccc
    15541 ccagtttggc atcaaacatc acataagttt tttcacaacg caaagcttca tatagcgtat
    15601 tcccacggtt ggatatcctg tcgctgggat tatcgcagac cagtgtgggg caggtgatct
    15661 cttgcaccct atcagcaaaa gtgtaagcgg ataatgcctg caaatagtca aaaggagtag
    15721 tcaaaccgtg tgtatacatt cttgatttga aataaaattc tttcattgga ttttctgaga
    15781 acaattgata aaaacaggca ttaagttcat cagcttttcc ttcttttaat aacatcataa
    15841 ggtttggagg tagcatttta ttgagtgagc ctgcaatatc catctgtcct ggatcacaaa
    15901 ttaatgcagc taatcgtttt tcactgcagg ccgcccttgg cgcaagataa ccgccaaaac
    15961 ttctgccaat gagcacataa ttggatgtcc ctagcccatt ttgctcatct agccaatcca
    16021 aaacctgaga gacaacatgt tcgaaatcgg ctctcatata caacttttgt cgacgtaaaa
    16081 catgtccttg tcccggacca tcaaaaatca aaatatgata tccatgtaaa agcgcagccg
    16141 ggacgagggc ataattttct tcaaaagtac tgtcatatcc tccaggtacg atcaaagttg
    16201 ctttggggga ttggtcgctg aaaaataaat agccgctcaa cgtttgatca tcataaggga
    16261 tccatatttt ttgagatcca ggatgcaatc gatgcattgc tcgttcaaaa caaaattgca
    16321 attgatcaaa acaaggaatc acacgcggat catcaggatt ggcacgtaaa aaaaactcgc
    16381 ttgcccggta atattctgcc gcacgcagat aagtgtatgc ggctgttata aaatgattgg
    16441 cttcccagct gatattggct ttggttttaa gtatatctgc catctcatac catgccttat
    16501 accaactttc cccgtcttta tcagtgattt tggacaaggt tgtataacat tcaccaacat
    16561 cagcacaccg cacgtaacat ttaccaagag ttcttaaaaa ctgtccgaaa aattcatcac
    16621 tgaaaattcc tgttttttga tacatatcat aataattagc atgcataata gtaaaaaatt
    16681 ccctaaaaat tacagagtaa ctgaaagtaa aatggattgc catgtgccaa agtatattac
    16741 gcccaggcct atgaattatc ctaaatgata ttaggacaga cttatgaaaa ccccaaggtc
    16801 aaggcaaaaa atgtttttaa tgagggagtt tagataaact aaatgactga attaaaaaca
    16861 ttttttaacg acgagattgg gggttttgca agtctgtagg attcattttc aggggagtgc
    16921 tgtctaaaca caatctaata ccgataaaga tctttgcata ctgaagacac tgcgttttgc
    16981 atttcgtcat cattaataaa atctgactgt acaattttct gatgcatcaa ataatgaaaa
    17041 tactgataag ctttctttaa agttgtgaat tgagatttcg ttaatacgtt cgataaaaac
    17101 aaatgcttaa gttggcttag tgtattggta taacttgaaa atttgggatc cctaagattc
    17161 aatataagaa attgaaccaa aaattccaga tccaataatc ccccccgagc tagctttact
    17221 tcatctgact cttgaaattg atcgatttta gatcgcatgg ttaatacgtc cctttgcaat
    17281 aatttactat ctctctctag caacaggact gactttttta gttgggaaaa ctgatttttg
    17341 atctttaaat ttcccgataa tatcctcgct tttagcaaag cttgatgttc ccatgtccag
    17401 gcttgtgttt tttgatactc aacaaaagca tccacatgac tgactaacaa gcctgcagca
    17461 ccggagggtc ttaaacgagt atcaacgtga tacaaaaccc ccatttgagt acgagtagtc
    17521 agcatatgca atattttttg tgttaatcgg gtcactaaag cttcttcaga tggtttggca
    17581 gaatgtaaaa agaccaaatc gagatcagac gcatagctca tctctcgact gcccagtttt
    17641 ccataagcaa taatggcaaa tcgtgattga atttgagcca tttcagggtg acggccacat
    17701 aactgtttgc ttgcaataat taaaacttga ctgacaatga cttgcgccac atcagataga
    17761 aatttcccta tgcgcacagc gtcacataat ccatacaatt ccgctctggc agccaccatc
    17821 caattcgtta atttaaactg acgcaggatt tcctcatgca cttccacatc attagtatgc
    17881 tctaattttt cagctattgt ctgttccatt tgggataatg aatcaggatg ccaatccttt
    17941 tcctgctcca acaagatttc caacaaaaaa ggttggctaa caaacaggga agaaataaaa
    18001 ggactattca caaaccaaaa taataattct cttagcgcat gcgggttttc agtgagtaaa
    18061 gccaggtaag cacttcgccc cacaatattt tcaagcaaat gaattacttg taataaaacc
    18121 tcatccgttt tagggcagta agtcaattct gtaagtaata aaaccatgaa acgatcaagg
    18181 cgaatgcgag cgccctgtga taatcttctg caccgtggcc catgtcgaaa agcatggagc
    18241 atttggtaac aatgttgtgc gttttcatac cctaaactaa ttaataaatt cactgccata
    18301 ttactttcaa catgaccttg ccaaaggcta aataattgat tgtttaacaa tctcttttcg
    18361 tcttcataat catcaacctt gccaagaatg gaatgaaaga aattactaac aattcgttga
    18421 tattgttgta atttattgaa aaattgttcc cagtcagtga atcccatggc ataaactatc
    18481 tgcacttttt ttaactggtc ttcaggtaga gaatgggttt gttgatcgtt caaactctgt
    18541 aaaatatttt ccaattttct caaaaaaaga tatgcttgtt ttaaagcatc acacctgggt
    18601 agtaattttt ccttttttat cacagccagc gctaacatgg cattttgaac ttgcaattga
    18661 ggtaatctgc ctccgcggat taattgaata ttctgaataa taaattctat ttccctaata
    18721 ccacctcggc ctcgtttaat atcattcaat aaggggttaa gttgcacctc acgctcgatc
    18781 attgccttca tccctcgtag ggattctatc actccaaagt ctacatacct cctgtaaaca
    18841 aaagggatga ttaaacgctc aaaccatgga aatggttcat tcaaagactc agcaataact
    18901 cgcgctttaa ccatggcata acgttcccaa tcacgtccct gttcctggta ataagtttcc
    18961 atagctgcaa aacttgaaac caaagggcca ctatctccat taggtcgtaa tcttaaatcc
    19021 actctaaata caaaaccatc aggcgttaca ctctgtaata aatgaacaaa tgtttgcacc
    19081 atcttgctaa aataatgctg attggtaatc tgctcctcac catcagtgct gccaacttca
    19141 ttgaaggcaa aaatcaaatc aatatcggac gaataattaa gctctcttcc cccaagtttc
    19201 cccatggcaa gagcatataa ttgaatttct ttaccctcac tgtttctggg aaccccgtag
    19261 cgactcaaca agttaaatcc aatatatttt aaagtatgca agatgataga atctgccaga
    19321 tcagaccagg attgcataac ctcttgagta gaagccaaac cggttatttc aagtaataac
    19381 aaccgcaaaa aatgggtatg acgaaaccga cgcaattcct gcatatactg ggcttgagat
    19441 aattccaggc tgatttgctg aatggcataa aaatagcttt cgcgaggcca taaggaacag
    19501 caggaatcac tttctaaaag agagaccaat aatttaattt gtctgctggc atagtcactt
    19561 accaatatca acttttcggc actttctctc aatggatggt gcaaaggagc aacatatttt
    19621 tcaatcaacc aggattttgt agaaatcaat gtttcaatat cgctttgtg
//
LOCUS       DBNCWK010000048        19264 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.048, whole genome shotgun sequence.
ACCESSION   DBNCWK010000048 DBNCWK010000000
VERSION     DBNCWK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19264
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.048"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(220..1593)
                     /locus_tag="AC06IO_001503"
     CDS             complement(220..1593)
                     /locus_tag="AC06IO_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HIO9874698.1"
                     /translation="MFQSILIIALMTFSFITQAALPADPIQRCLDIRRLVDITSRQKM
                     AAKEPGVLRFKFHKISASELPLDNPVGNMDEVIQALNNQIENCNNSYGEFQTVTIAGH
                     QFKRQEWCLNTNKKMLTLAKAAHGDFKKFLSSIKTEFDWYKSDGWPENHAGFKKGEFQ
                     FTAYYAPAAVEARSKCGGAFLYPLYSNPGVVSVALESKKYNLKTPLCGVDPLSKVMRG
                     FCLKNADGTYSVAPDREEIEHGALNPKYIIGYLKDPNDSAFLMLQGSGSLLLDGKLFH
                     INYDGANGRPRTMLGRIVQCAQDPTCGGNLDTMERCAKDPKCHDEAKLRCNVSKKIRQ
                     SAASEKLIRQYLNNLTPDKADNLRNRDQSYVFFVKEDGGPYGSENISLTPHVSCATDH
                     RVIPVGMSFIYHCKKATSWCVAQDAGGAIIGAHVDVYKGEGNQAGVEANQLNHTGTLF
                     VALPKRK"
     gene            2111..2776
                     /locus_tag="AC06IO_001504"
     CDS             2111..2776
                     /locus_tag="AC06IO_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874699.1"
                     /translation="MTLILKMKKFKLMAKEQCFAFIKNAVNVGMDEVIYSNGTNKTFI
                     TDGLATCAAVAIYGSDSGCSAAFTHMSSEASDKDDKRKETILNEMLEYVLKSNSLSDV
                     KLIISPSRIQEKHLISFILEWVKQKKIAYSLLETGGDSAVFNIDMDSRVLMLSTSLQL
                     KQDVSDKTWHGHGIIIGQSEGIPVIEKHKKHFVFFAEDISSQITKFSDPPKTVGLAAA
                     VSA"
     gene            2909..3334
                     /locus_tag="AC06IO_001505"
     CDS             2909..3334
                     /locus_tag="AC06IO_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874700.1"
                     /translation="MRRHFIIIVLGIFQFGCIPLAEHHNKISPATAHEEYHEVNFSNL
                     YEKNRSFPALETGIYAILDGILISSSQNTHLGKKQSKLLEEYVRQNQLFCQKGFDQDW
                     MNQLKQNLDRLEKTENNRIASAVNKKMQTLFAERQCNAL"
     gene            3601..4434
                     /locus_tag="AC06IO_001506"
     CDS             3601..4434
                     /locus_tag="AC06IO_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2541 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874701.1"
                     /translation="MLMEFEDWFLEQISNGKKCILIGENHYSYASEDCLDCILKLQRQ
                     GKINKKIVFFDENLAALDNLEPYTIESLSKLNTVEPSLIALIKNNVEVYGLEDNFSNP
                     FKNLKSIKKVTARLKDLGLQIGNRIFNDFEHCLYWACHLYGKSLVRVQRGNEGFCNQI
                     LHKMDEDTIVIARVGAKHIPTVRKDGVVIEEGMLQRLGKDMASACFITHFPKNRTYSE
                     QKTYQPESDAFGSYDSIECCMVQKKMIHQPDLTLWAEQDKQRKDTNDYKDTKKHRCTI
                     M"
     gene            4550..4933
                     /gene="mscL"
                     /locus_tag="AC06IO_001507"
     CDS             4550..4933
                     /gene="mscL"
                     /locus_tag="AC06IO_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIO9874702.1"
                     /translation="MSLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIITALVDGIIMPC
                     VGLLLGGVNIAGLSFTVGDAQIKWGNFLQNVIDFIIVAFAIFVLIKLINLLQRKKANE
                     PEPVTLEIQLLTEIRDLLARNSSKI"
     gene            5145..5552
                     /locus_tag="AC06IO_001508"
     CDS             5145..5552
                     /locus_tag="AC06IO_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2539 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874703.1"
                     /translation="MKLIINKKNRAESMALDVEATTTVNELLLILYTHRSKRYEPLSF
                     SEYQKFISVYQCLLYGDQFINKLLPGTNLADYQLEDPCHLTWEEFTEKQCLLADAETS
                     RILLWQANKKASKPEIEKDLMSVASSDAMNPDM"
     gene            6133..7549
                     /locus_tag="AC06IO_001509"
                     /pseudo
     CDS             6133..7549
                     /locus_tag="AC06IO_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948240.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2538 family Dot/Icm T4SS effector"
     gene            complement(7673..8692)
                     /locus_tag="AC06IO_001510"
     CDS             complement(7673..8692)
                     /locus_tag="AC06IO_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9874704.1"
                     /translation="MIVDFETHFITEACIDYLTQRQEVPKLVPELNGAYTMCFTPDVS
                     LFHTSALMEELLSLNEQRLAIMDQAGVTIQVLSLTTINGIDSCPGDENKSTALAREVN
                     DQLYSAIQRHPERFKGFASISPYDVKEGVKELERAISQLGFVGWLTHSNFGEDNYLDD
                     KTYWPLLEAAEGLNIPIYLHPNVPIMREFGKYGFALGGSALGFEFDTALCLMRMILGG
                     VFDAFPKLKIMLGHLGETMPFLMERLDHLYRIPDLKAYRPSIQRIPSEVLRQNVYITT
                     SGRFFVPALRYVLEVMGEDRVLFASDYPMESLLDATRFIQDSDLSNQTKQKIFSINAK
                     NLNLI"
     gene            complement(8917..9873)
                     /locus_tag="AC06IO_001511"
     CDS             complement(8917..9873)
                     /locus_tag="AC06IO_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIO9874705.1"
                     /translation="MSTVRFNNQSFALTPDESLLQCFLRHGVEYPNSCQAGICQSCLI
                     KAKDGEINPDWQEGLPETLKSQGYFLACLAKPSTSLYVASPDNAECETQAQIMSLQRL
                     NHNVIQVKLKVEHPAPWLPGQYLSLINPEGTMRNYSIANIPADDGYIELHIKIYPQGK
                     MGQWLLQRAAINTLIAIRGPFGQCYYHNPHNLAFDILLAGTGTGLAPLIGIIRSALTQ
                     KHEGTITLVHGGVTDRDIYYKEELEMLSLLFSNFRYDPCVLQSQGLYPEGSIEKRVLT
                     HLHSPNTTKVYVCGPKETTNKLKTRIFLAGVPSTNIYSDVFL"
     gene            complement(9870..10244)
                     /locus_tag="AC06IO_001512"
     CDS             complement(9870..10244)
                     /locus_tag="AC06IO_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group I truncated hemoglobin"
                     /protein_id="HIO9874706.1"
                     /translation="MTESLFERLGGQNAVNMAVDIFYRKMLMDDRVNYFFDDVDMEQQ
                     ILKQKGFLTMVFGGPNQYTGKSMREGHQHLLARGLNDSHVDIVIEHLGETLKELGANE
                     EDIQKVAAIANSVRGDVLGRSS"
     gene            10325..10756
                     /locus_tag="AC06IO_001513"
     CDS             10325..10756
                     /locus_tag="AC06IO_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIO9874707.1"
                     /translation="MQLTQFTDYSLRALIYIALKKGSCTIKDITDAYTISNNHLIKII
                     HNLSKMGLIKTIRGKHGGILMAASPETINLGQLIMELEPNFDLVPCFNKEKANCCIAP
                     VCKLKSVLYEAQRAFLQILEQYTLADVLHNPSELSALLRIS"
     gene            complement(10789..11685)
                     /gene="tehB"
                     /locus_tag="AC06IO_001514"
     CDS             complement(10789..11685)
                     /gene="tehB"
                     /locus_tag="AC06IO_001514"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HIO9874708.1"
                     /translation="MPREYDNLICYKQLSIDDHGKLKFFLEKHSTKEGTWGKLSLNEG
                     EIDFIFQNGLGQELSRIRLDKNNRQLSIPPASWHKIIPISASFDAQLSFYCKPHRYFN
                     KKYGLTEVHRDLLYVYHTYLSHLGSLTTLDVGCGSGRNLLYLAQLGHRLTGIDINQTA
                     LENIQIIAQKENLSPLNILSQDLNQPQSLAPDHYDFVCSTVTLQFLNASRIPSLLIEM
                     QEATKKNGYHFLVFPVQSELYSLPSSFTFLPKQQELYHFYQDSGWSILEYKESVGHLH
                     KQDELGRPVQGLFALLLAQKVI"
     gene            complement(11863..13029)
                     /locus_tag="AC06IO_001515"
     CDS             complement(11863..13029)
                     /locus_tag="AC06IO_001515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIO9874709.1"
                     /translation="MNHCEKKNLPKDSATVAINSLAQQKINAGIKIHNLSAGEPKLPP
                     HPSIINAVTQAMEQGHTLYPPIMGIPELRHMGCEWMYRSYDCSFQNEQCLVVNGGKLG
                     IYLLMQLLLQPNDEVIIPSPYWVSYPAMVSLFGGTPVPVETTEAEEWKLTPQALKKAC
                     SAKSKILILNNASNPTGALYTQSELIHLLQVAEEHNLLVISDEVYSELTYDGHHYVSC
                     GSLPQFRERVIIIQSCSKNFSMTGWRVGFIFAPEIFIKPLSALVSQSTSGVTTLSQWA
                     AVAALQEVNQVNIWVQQNMEKRRNCLLRTLHDYLGLTITPPISSLYVFLSLKDIGCKN
                     QNSEEFCKQALDKANVAMVPGIAFGKEGYVRLSFGGNEDDLKSGIYALAQWLHR"
     gene            complement(13029..13289)
                     /locus_tag="AC06IO_001516"
     CDS             complement(13029..13289)
                     /locus_tag="AC06IO_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIO9874710.1"
                     /translation="MSTIEELRKQIEQTDAYIIEKLAQRQELSKQIGALKAKEGKKII
                     DRLREKKLFEYYDYLSRQYHLQQDFVNQLFKIIISNSKKVQK"
     gene            complement(13324..13908)
                     /locus_tag="AC06IO_001517"
     CDS             complement(13324..13908)
                     /locus_tag="AC06IO_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HIO9874711.1"
                     /translation="MKNLVEEKEAIRFGVSGDIGSFSEEAALLYAKEKDINVHLIHLL
                     DMEGVLNAIENETIDLGILPVVNLLGGLVKPAFQAMGKYSFTPIDEIWHDINQCLLVK
                     PNTQHHKITHIVSHPQGLAQCKKFLRHKFKDAKKIEWIDTAKAARDLAEGILPPHTAV
                     IASARCAPLYGLQMRARNIQDSYQNKTAFILVKK"
     gene            complement(13905..14948)
                     /locus_tag="AC06IO_001518"
     CDS             complement(13905..14948)
                     /locus_tag="AC06IO_001518"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIO9874712.1"
                     /translation="MSYSILKKLPPIEKIIRELPLSHPAYLQIAQDRQEVKAILEGRD
                     KRLLMIVGPCSAWPKKAVLEYAERLVQLNSKVKHALKLIMRVYIQKPRTTKGWTGPVN
                     QPDLFSTPDIEAGIKYTRDMMIKVIQMGLPIADEALFTHNAKGFLELLSWVAIGARSS
                     EDQEHRIFASALDCAVGLKNPTHGSLAVGVNSLVASQHPHVAVFDGYEVQTHGNHHAH
                     LVLRGSNQAPNYSVSHLEEVMYYMGMHQIVNPSVIIDVSHDNCLVNGKKNHQLQPEIA
                     FNILESIKNRPDLKQLVKGFMVESFIKEGNQKLDSINPDNIDLSGLSVTDPCLSWEKT
                     ESFLLELAEREAL"
     gene            15470..15721
                     /locus_tag="AC06IO_001519"
     CDS             15470..15721
                     /locus_tag="AC06IO_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874713.1"
                     /translation="MSICWDLIINHNTLLQTNFTGHRVQMNDSESTPKGILFVSNKLF
                     IDLLTYITEYQSITRKELMNLSNNEIKPRRKSNKRLLMW"
     gene            15715..17433
                     /gene="lotC"
                     /locus_tag="AC06IO_001520"
                     /gene_synonym="lem27"
     CDS             15715..17433
                     /gene="lotC"
                     /locus_tag="AC06IO_001520"
                     /gene_synonym="lem27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase LotC"
                     /protein_id="HIO9874714.1"
                     /translation="MVRVLNLQKGVNMFNVDNSGKGNCLYYAYSISLMYYLRAKNNVR
                     ITEDIFNKLGLKEEDRARLRKLLSKDPDRAFTRDEIKTIIEPILGRATRDLAAEHTKV
                     EFKSSPHDTPLFSSLHYAVEFGFKRSLQINESELTLLIDNDFSNPDYTEAEIYKVSGL
                     LDALQEYILTRTPSVIEEFNRQWENKKQELKEQSLTEKEIQIHQATILDNILRKETID
                     FLLADNEKHLDEYREHLRREFVWGSEETLMVLHRAIQGERMVRNHEGRIEPVYDHEII
                     LHVHRNGASPSYQAGSPEMILNNEGNVHWTSIIPDAIFTSKLTDKEEKLLEMLERMQS
                     EYGSKELGVEKYSSISDWLSDLMKQIELIKTSPTAVTKQKEIELFFQLLAKATPKLAS
                     EPALRMSLGTLFSNFLECIPALMVENKLSTGLAKFGLYSPVISQKKEDLSLTKSSEEK
                     VNSEQPQKGVLSQFSKHTLLKVSEDVSPKPYPTELNPYVRKNKQSGPRVARALTQMYQ
                     KGLEGDERYTPEKCKDIAQRYIGYSKSHKRNFILDDVILFVQEMDKIIKQQESISENS
                     SKRAAM"
     gene            complement(17591..19141)
                     /locus_tag="AC06IO_001521"
     CDS             complement(17591..19141)
                     /locus_tag="AC06IO_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIO9874715.1"
                     /translation="MSPRICCYNQYPGLYQDFDAMREDLQRIQKMGFKQVWVNPFYTP
                     CQYNPVPNMANRIHSPYAMQDESIYPRYAKNDKSVQQYTAKANELGLMPIFDLVARHL
                     AVDHRFVNGDKFFLEKYNIDTKKWFKRHPNGNLVMHNMDENYNPLANNPWSDVATFNY
                     DDPVICEQIIEYYWKPFIERNIEHLGFQGIRIDAPAMVNNKVLSRLTEIAKEACQHKF
                     QRDPLIIAETIGRGYMEENLALKGIVTHTMNSVFWMPGPEGGYGYSYNLWQQDDNWFT
                     QNKGLLQQVGPTIGFPGSHDEPRYIQQLVEKGIHDDKLLAKRMREKLAVSAFCSDGGW
                     ILQYGDEYGATKPVNVFDPTPVEYHQQNHLRRFDLSDYISEINTTLAQLPNPHFPEWA
                     QRVFLPNHPDLVTFIVHQGWGYTGTSFVIATNTDPSQQIQLTEKELGEIMLANGRNNT
                     TEKQIKPQVLYLCGAVRASPELKKEIQIVTSRPATSEKSIHFFQSHPEEMQMSEKLPL
                     EKTPPKFK"
BASE COUNT         5926 a         3507 c         3867 g         5964 t
ORIGIN      
        1 ggttttaatg gcgagcttgg gcgatgttaa tcagtacccc aatggaatca ttttgttctc
       61 agatcatagg aaaaagctta gccgcatttt ccttgctcat tgcgcaaagt aatccaaatc
      121 aaaaagcatt cttatttatt cagttgtaga ttaacagcac cctaaatgac catagccaat
      181 aaaagtaatt actcgaaata agtattggtt gccttctttc tatttccgtt taggtagtgc
      241 cacaaataac gttccagtat gatttaactg atttgcttcc acacctgcct gatttccctc
      301 acctttatag acgtcaacat gagcccctat aatggcgccg cctgcatcct gagccacaca
      361 ccatgaagta gcttttttgc aatgatagat aaaactcatt ccaacaggaa taaccctgtg
      421 atcggttgca catgacacat ggggggttag agaaatattt tctgaaccat aagggccacc
      481 atcttctttg acaaaaaata catagctttg atctcgattc cgtaaattgt ctgctttatc
      541 aggggtaaga ttgttcagat attgccgaat gagcttttct gatgcagcgc tttgcctgat
      601 ttttttagat acgttgcagc gtaacttggc ttcatcatgg catttcggat ctttagcgca
      661 acgttccatt gtgtcaagat tgccaccaca ggttggatct tgagcgcatt ggactatgcg
      721 accaagcatc gtgcgtggcc tgccgttggc accgtcgtaa ttgatgtgaa acaatttgcc
      781 atcaaggagt aatgaaccag agccttgtag cattaaaaaa gctgagtcat ttgggtcttt
      841 aagatagcca attatatatt ttggatttaa agccccatgc tcaatttcct ctcgatctgg
      901 tgctacagag taggtgccat cggcattttt cagacaaaat ccacgcatca ctttgctaag
      961 cggatccact ccgcaaagag gagttttcaa attatatttt ttactttcta acgcaacgct
     1021 aacaacaccg gggttgctat agagaggata cagaaatgct ccaccacatt tggagcgggc
     1081 ctcaacagcg gctggagcgt aataggcagt aaattgaaac tcaccttttt taaatcctgc
     1141 gtggttttcc ggccagccat cgcttttata ccagtcaaac tcggttttga tactcgataa
     1201 aaatttttta aaatcgccat gggcggcttt tgccagagtc agcatttttt tgttggtatt
     1261 taagcaccat tcctggcgtt tgaattgatg gccagcgatt gttactgtct ggaattcacc
     1321 atagctatta ttgcagtttt caatctgatt atttaatgcc tggataactt catccatatt
     1381 acccacaggg ttatctaacg ggagttctga tgctgaaatt ttatgaaatt taaaacgtaa
     1441 caccccgggt tctttggctg ccattttctg tcttgaggtg atatccacga gccttctaat
     1501 atccaggcag cgctggattg gatcagcagg tagggctgct tgagtgatga agctaaaagt
     1561 cattagtgca attattagaa tggattgaaa cattggtatc ctttagctta gctatggttg
     1621 aggcattgcc cgtccattga tttcaattag taacatgaat tatataggct attggccaac
     1681 tcatcaaaga gatgaattta cataaattct gccaccttat aactacctta tcattataca
     1741 gaaatagtgt cgaatcccaa ttgtcccttt ttaaaaagcg tttcaaaaca aaagcttaca
     1801 tacatcagtg gatgctctta acttatctgc gattgaagca cttcttctta agcttgaaac
     1861 tttattgtcc agaaaagact ttttaagtta ttgtcaaata tggcgtatag caccggttag
     1921 cctcagtgtt tggttgaacc tagcgattga ttaatcatag tgttcaacat aaaaataaac
     1981 ccataatttt ttattgggtt tatttaacgg gataacacgg ataaattttt cataaaaatt
     2041 atatgatttt cttttcataa gattcatttt ccatttaaaa tggaaaaaat aatgacaatt
     2101 tggtttataa atgactttga tattgaaaat gaaaaaattt aaattgatgg cgaaagagca
     2161 atgttttgcc tttataaaaa acgctgtaaa tgttggtatg gatgaggtta tctatagtaa
     2221 cggtacgaat aagactttta ttaccgatgg attggctacc tgcgctgctg ttgctatcta
     2281 cggcagtgat agcggttgtt ctgccgcctt tactcatatg tccagtgaag cttcggacaa
     2341 ggatgataaa cgaaaagaaa cgatattaaa tgagatgttg gaatatgttt taaaaagcaa
     2401 ttcattatcc gatgttaaat taattatttc gccttccagg attcaagaaa aacatttgat
     2461 ttcctttatt ctggaatggg taaagcaaaa aaaaattgct tactccctgc tagaaacagg
     2521 gggtgattca gcagtattca atatagatat ggacagtcgt gtattaatgc tttcaacttc
     2581 actgcaacta aagcaggatg tatccgataa aacatggcat ggtcatggta ttatcattgg
     2641 ccagagtgag ggtattccag tgattgagaa gcataaaaaa cactttgtat tctttgcaga
     2701 ggatatttcc tctcaaatta ccaaattttc agaccctcct aaaacagttg gtttagcagc
     2761 cgcagtaagt gcttagaaca agcacgagct ggataaaatg aaatgaattt cgtatcttag
     2821 tcaaaattga aatatttcct caatgctgag tagatatgtt aatttcataa tagattgttc
     2881 tttatgctca atttgcaggt ggtgtatgat gagaagacat tttattatta tcgtgcttgg
     2941 tatatttcag ttcggttgta tccctttggc agaacatcat aataagattt cacctgctac
     3001 ggcgcatgaa gagtaccatg aggttaattt ttccaattta tatgaaaaaa atcgctcttt
     3061 tccagcatta gaaaccggca tttatgctat tttggatgga atcttgattt cctcaagtca
     3121 gaatacgcat cttggtaaaa aacaatcgaa gcttttggag gaatatgtac gtcaaaatca
     3181 attattctgt caaaaaggtt ttgatcagga ctggatgaat caattgaagc agaatttgga
     3241 caggttagaa aaaaccgaaa ataatcggat tgcatctgct gtaaataaaa aaatgcaaac
     3301 gctgtttgct gaacgacagt gcaacgcgtt gtaaccaagg ctcgtcattt tatcaggcag
     3361 cctggttcaa atcacatgtg aactcaatct ttcgaactat tgatcacagt attttttatg
     3421 cagcatatca tgtcatgaaa ggggcatgcc attttaattc catgctatct agtccaggat
     3481 aaatcaggaa agacatacaa gcagatgtgg aaatatatta ttcatattga cttatttttg
     3541 attaaaatat acaataatca acttcattaa tttgtagttc tattttattt ttagtgagat
     3601 gtgctaatgg aattcgaaga ttggtttctg gaacaaataa gcaatggtaa aaaatgcatc
     3661 ttgatagggg aaaaccatta ttcctatgca agcgaggatt gtctcgattg tattttaaaa
     3721 ttgcaaaggc aaggtaagat aaacaagaaa atagtatttt tcgatgaaaa tttagctgca
     3781 ttagacaatt tggaacctta cactattgaa tctttgagca aattaaatac agttgaacct
     3841 tctttaattg cattaattaa aaataatgtt gaggtttatg ggttggaaga taatttcagc
     3901 aatccattta aaaaccttaa atctattaaa aaagtgacgg cacgtttaaa agatttgggt
     3961 ttgcaaatag ggaatcgaat atttaatgac tttgaacact gtttgtactg ggcttgccat
     4021 ctctatggaa aatcattggt cagagtacaa cgcggtaacg aaggattttg taatcaaatt
     4081 ttgcataaga tggatgagga tactatagtc attgccagag taggggccaa acatatccct
     4141 acggtacgaa aagatggagt agttattgaa gaggggatgc tgcaacgatt aggcaaagac
     4201 atggcttctg cctgttttat tacacacttc cctaaaaata ggacttattc agaacaaaaa
     4261 acgtatcagc cagagtccga tgcttttgga agttatgatt ctatagaatg ttgcatggtt
     4321 caaaagaaga tgattcatca gccggatttg actctatggg ctgaacaaga taaacaaaga
     4381 aaagatacca atgactacaa agatactaaa aaacaccgtt gcacaataat gtaagtttaa
     4441 attcccttct acaagctaac tggggtcagg acagcctgta attgaaaaat tggaaaaatt
     4501 ttgcgaatat acatttcatc gcataactta atcatgatag ggatgtctga tgagtttact
     4561 aaaagaattt aaagaatttg ccatgcgagg caacgtgatg gatttggctg ttgctgttgt
     4621 aatgggtgtt gcttttaata aaattatcac tgctttggtc gatggaatta tcatgccctg
     4681 cgttggtttg ttattgggag gcgtcaatat tgcaggattg agttttactg taggagacgc
     4741 tcaaattaag tggggaaatt ttttacaaaa cgtcattgat tttattatcg ttgcttttgc
     4801 tatttttgta ctcatcaaat taatcaatct gctgcagaga aaaaaagcaa atgaaccaga
     4861 accagtcact ttggaaatcc aattacttac tgaaatacgt gatctgcttg caagaaatag
     4921 cagtaaaatc tgaaattggc tggcaaaaat aaacctctat ttatttgcag tcagatatcc
     4981 ccattcaaaa tgatttgtta aacagcttat gttttatttc ggtgagatgt atacagaatt
     5041 ctggtgtttc aatcggccac ttaaatctca caaagccata tattgattta ttgttattta
     5101 aactatacta gcagacaagg ctaattttta cttaatgatc gcaaatgaaa cttataatta
     5161 ataaaaaaaa tagggcggag tcgatggcgt tggatgttga agcaacaacg acagtgaatg
     5221 agttgctttt aattctttat actcatcgca gtaaaaggta cgagcctttg tcttttagtg
     5281 agtatcaaaa atttatttcc gtttaccaat gtttattgta tggggatcaa tttataaata
     5341 aattacttcc gggaaccaat ttagctgatt atcaacttga agatccatgt catttgacct
     5401 gggaggagtt taccgagaaa cagtgtcttt tagcggatgc agagaccagt agaatccttc
     5461 tttggcaagc taataagaaa gcaagtaaac ctgaaattga aaaagatctt atgtctgttg
     5521 caagcagtga tgctatgaat cctgatatgt aacaagaata aaacgtcctg tataacatag
     5581 tgagtgttcg ttttgtaatt cattacttga gtcatgcaat gaaaaaatta aatacatcca
     5641 atcagttaac cagtgaaagg tcttgagtcc cctatggcct caatataagg tgttgcccat
     5701 ttaataaatt cttaagcttt tgttgttaaa attttgctca ctaaaggatc ataaaaaatg
     5761 atctttttgc tgcggcctat cttctccaca aggatatacg gaggactagt ggtaacactt
     5821 agctttaatt aagtaaacgg gttcgtcgct gaggcaaaga aaatgagcga tttactctcg
     5881 gatttggtga ttgagagtag tttattaact ttaattcagc gaggcgttac ttaacaaatc
     5941 gtcgtttcct ttctttttgt ctctaaattg aaatatctat ggcctctgtc agtctttatt
     6001 aggcagaggg tttgttaatg tataaggaat tcataatgaa aaataatgat tttaataatc
     6061 agttgtttca ttgagataga gcaaggaatt gtcattatga aatcaatgaa aatcatctcg
     6121 gtggacaatt aattgattcc agtccaggtg atgagaaaaa ttcatgaatt agtaatgata
     6181 caagctgcag ccagggtttt ttatgaaaac aaaatatgaa ataagtcaag ataaaactga
     6241 gtttttggct aaagaacaaa gtagttctta tccagggtat caagtcagcg ttctggatct
     6301 cgaaaaaatg gttaagcatt atcaggaaaa atacggtatt cgcttgatta ttaatggcac
     6361 gacacccaaa tatcaggctc tgattaaaga gcgccaggtt aattttgaac aacaaaaaca
     6421 gcaattcctg gaacttaaat acgccaagtt tttacaaata ttttttcagc cacctaatct
     6481 gaatggcgca aactctcctt tttctatcaa taaatatatg ggtgcattta taggttttta
     6541 cgaagaaata tacaataaag ttcttccatt tctggatgcg aaaggaaaag ttatttctgg
     6601 attatccctg gaagaattaa gacaactcaa tgaggcctgc caagagctgt catgcaaagg
     6661 aatacttgat gcaacaattg atgaatttat agaacgaaat tctgattata tggggttaac
     6721 tgccagggaa agcgcaagtg aaatgaaaga tatctgtgat gaactgcaag aaggagaggt
     6781 tttgggttat ttctttactg gtcaacgtac cagtgggaga tgtcatttcg atctctatat
     6841 ctgtttacct ggtaaggcca taagaccgat attttataac acagcgctta tcagatatca
     6901 tgatcttgga gggatgtttc atttgaattt tccttttgtc gaaggcaatt tttttacacc
     6961 tgatttgtta aaattatatt ctgcaatgga cttgcaacag ctaatccctc aagctgacag
     7021 gacttcatgt ggtactttga caatgatgta tgccaaggag ttattaaaag atgatgcgag
     7081 agggctgaaa gagtttacgt tgagttttac ttattataat gagaaaggag aaaaggaata
     7141 tttttttctc ccatcaccac aagtattacg atattctcaa ataagtttat ataatgaagc
     7201 gctgaaagcc attttatctc atgaaaatga tggacaggct ggtttggtca ggaaaggcgc
     7261 taaaaaatat atgtttcaca ctatcgaaaa aattctcatc caatctttta agattgcact
     7321 tgagaaagaa gatgccgatg ttctggagga aaatcaaaaa atttgggata tattacccag
     7381 cttccaggaa aaatggcagg aggcttataa ggaaatggtt gccatacgcg atgtgatgca
     7441 ccaaggggta aataaatatt tgctttattc cacccatcgg atgagtcata ttgcaagtga
     7501 tgagtccata aacaatgaag cggatgctga tagattaatt cttcgttaaa aaatattgca
     7561 gaatgaaact attcgtattt tatttctata aagcatgaca ttaattgagt ctggctcaat
     7621 taatgtctgc aagaaaatca gctctttact gatatgatgc aagagagatg attcatatta
     7681 agttcaaatt cttggcattt atcgagaaaa ttttttgctt cgtttggttt gataaatcgg
     7741 aatcttgaat aaatcgagtc gcgtcaagta aggattccat tgggtaatcg gaggcaaaca
     7801 ataccctgtc ttcccccatg acctctagaa cataacgtaa ggcgggaacg aagaaacgtc
     7861 cgctagtggt tatgtaaaca ttttgacgta atacctctga gggaatacgc tgaatagaag
     7921 gtcgataagc ttttaagtct ggtatacggt ataaatgatc gagacgctcc attaaaaacg
     7981 gcatggtttc acccaagtgc cccagcataa tttttaattt gggaaacgca tcgaatacac
     8041 ctccaagaat cattcgcatc agacataaag ccgtgtcaaa ttcaaaccct aaagcacttc
     8101 cccccaaggc aaaaccatat ttaccgaatt cgcgcatgat gggtacattc ggatgcaggt
     8161 agataggaat gtttaaacct tctgcggctt ccagcaatgg ccaataggtt ttatcgtcca
     8221 gataattatc ctcgccaaaa ttggagtgtg taagccaacc tacaaaacct aattgagata
     8281 tcgctctttc taattctttt acaccttctt tgacgtcata aggcgatata gaagcaaaac
     8341 ctttaaatcg ttcaggatgt ctttgaatgg cagagtaaag ctggtcattg acttctcttg
     8401 ctaaagcggt gcttttgttt tcatctccag ggcaggagtc aatcccattg atagtagtca
     8461 aacttaatac ctggattgtt acccctgctt ggtccataat tgccagtcgt tgctcgttta
     8521 aacttaataa ttcttccatt aatgcacttg tgtgaaataa ggaaacatca ggtgtaaaac
     8581 acattgtata agctccattg agctctggaa ccaatttggg aacttcctgg cgttgagtca
     8641 gataatcaat acaagcttcg gtgatgaagt gcgtttcaaa atcgacgatc atgaacgtaa
     8701 tccttttaga cgtgagaaaa acgattgaaa attacatgct taagtatagc cggaagatta
     8761 gataccgcca catggctata aaagttctga tggcaaattg ccggaaaacg ttttaaaaaa
     8821 aagaaatgct accagcggaa agaagagatc attttgatga gcgagaaaca taatctggtt
     8881 tgcaccagat tataaatcaa cagggctatt taaatattat aaaaaaacat cactataaat
     8941 atttgttgaa ggaacacccg caagaaaaat tcgtgttttc aatttattgg ttgtttcctt
     9001 tggaccacag acatacactt ttgtagtgtt gggagagtgc aggtgagtta gcacccgttt
     9061 ttcaattgag ccttctggat aaagcccttg actttgcagc acacagggat catagcggaa
     9121 gttgctgaac aataaagaca acatttccag ttcttccttg taatagatat ccctatcagt
     9181 aactccgcca tgcaccaggg taattgttcc ttcatgcttt tgtgttaatg cgcttctgat
     9241 gatacctatc agcggagcga ggccagtccc tgtccctgcc agcaatatat caaaagccag
     9301 attatgtgga ttatgataat aacattgacc aaaagggcct cggatggcga ttaatgtatt
     9361 aatggcggcg cgttgcaaaa gccattgtcc catcttgcct tgcgggtaaa tttttatgtg
     9421 cagttcaatg tatccatcat cagcagggat attggctatg gaataattgc gcattgttcc
     9481 ttcaggatta atgagcgaca ggtattgtcc tggtagccat ggggcaggat gttccacctt
     9541 cagtttgact tgaataacat tgtgatttaa gcgttgcagg ctcattattt gagcttgtgt
     9601 ctcgcattcg gcattgtcag gagacgcgac ataaagcgaa gtgcttggtt tggcaagaca
     9661 agccaggaaa tacccttgag atttgagtgt ttcgggtaat ccttcttgcc agtctggatt
     9721 aatctccccg tctttggctt taatcaggca tgactggcaa atgcctgcct ggcaggagtt
     9781 agggtattct accccgtggc gcaaaaaaca ttgcaagagg ctttcatccg gagttaatgc
     9841 aaaagattga ttgttaaatc gtacggtact catgatgagc gccccaatac atcgccacga
     9901 acactattgg ctattgcagc tactttctga atgtcctctt cattagcacc caattcctta
     9961 agggtttccc ctaaatgctc gattacaata tccacatgtg agtcatttaa gcccctggca
    10021 agtagatgct gatgtccttc ccgcatactt tttccagtgt attgatttgg tccgccaaac
    10081 accatggtta aaaatccttt ttgcttaagg atttgttgct ccatatccac gtcatcaaaa
    10141 aaataattca ctctgtcatc catgagcatt ttgcggtaga aaatatccac agccatgttg
    10201 acggcatttt ggccgcctaa tcgctcaaac aatgattcag tcatgatata ctccttaaag
    10261 atgtatttga aatacatctt ataaaaagaa ataatagacg tcaacaaacc agaaggattt
    10321 ttttatgcaa ttgactcaat ttactgatta ttccttacgc gccttgatct atattgccct
    10381 aaagaaaggt tcatgtacca ttaaggacat tacagacgcc tataccatat caaacaatca
    10441 tctgatcaaa atcattcata atttatcgaa gatgggttta attaaaacaa tacgagggaa
    10501 acacggcggt attttgatgg cagcctcacc tgaaaccatt aatttaggtc aattgattat
    10561 ggagcttgag cccaattttg atttggttcc ctgttttaat aaagaaaagg caaactgctg
    10621 cattgccccg gtatgtaaac tcaagagcgt tttgtatgaa gcgcaacgtg ctttcctgca
    10681 gattttagag cagtatactc tggctgatgt tttacataat ccaagtgaat tgtctgcttt
    10741 attgaggatc agttagcaat ccagggtaat attttatgaa agtgatcttt atataacttt
    10801 ctgggcaagc aacagagcaa ataatccttg cactggtctt cccaattcat cctgtttatg
    10861 aagatgtcct actgattctt tgtattcgag gatagaccag ccgctgtcct ggtaaaaatg
    10921 atacagctct tgttgttttg gtaaaaacgt gaatgagctt gggagtgaat aaagttcaga
    10981 ttggacggga aaaaccagaa aatgatagcc atttttcttc gttgcctcct gcatctcaat
    11041 caataaagag gggatacgtg aggcatttaa aaattggaga gtcacggtag agcagacaaa
    11101 atcgtaatga tctggtgcaa gggattgagg ttgatttaaa tcttgcgaca aaatattaag
    11161 tggactcagg ttttcttttt gagcgattat ttgaatattt tccagtgccg tttgattgat
    11221 atcaattcca gtcaatctat ggccaagttg ggccaaatac aacagatttc taccggaacc
    11281 acaaccgaca tccagagtgg ttagtgagcc taaatgcgac aagtaggtat gatacacata
    11341 caataagtca cggtgcacct cagttaagcc gtattttttg ttaaagtagc gatgaggttt
    11401 gcaataaaaa ctaagctgag catcgaacga tgcgctgatc ggaatgattt tatgccagga
    11461 ggccggagga atagatagtt gcctattatt tttatcaaga cgaatacgcg acagttcctg
    11521 gcccaagcca ttctggaaaa taaaatcaat ttcaccttcg tttagggaaa gtttgcccca
    11581 tgttccttct ttagtgctgt gcttttcaag aaaaaattta agttttccat ggtcatcaat
    11641 ggatagttgt ttgtagcaga ttaaattatc gtattctctt ggcatggttt tttatcctaa
    11701 ggtatcgata aataacataa tagtttttta gcctattgtc gaatgtatca attttataga
    11761 gccgttaaat ggagcaggtt attaacagca aaactgcagt aaaataatca gattaggcaa
    11821 gaggtctagt aatgcttctt gtaaagtaat ggattacttt aattagcgat gcagccattg
    11881 cgccagagca taaatacctg attttaaatc gtcttcattg cctccaaaag acaagcggac
    11941 gtaaccctct ttaccaaagg cgattcccgg taccatcgca acatttgcct tatccaaagc
    12001 ttgtttacaa aactcctcgg aattttgatt tttgcagcca atatccttta gggaaagaaa
    12061 aacataaagt gaggaaattg gaggtgttat ggtcaatccc agatagtcat gcaaggtgcg
    12121 gaggaggcaa tttcttcttt tttccatgtt ttgctgaacc cagatattga cttgatttac
    12181 ttcttgcagc gcagccacag cggcccattg gctaagggtg gttactccgc tggtgctttg
    12241 actgaccagg gcgcttaaag gcttgatgaa tatttcaggg gcaaaaataa aacccactcg
    12301 ccacccggtc atggaaaagt tttttgaaca gctttgaatg atgatgacac gctcccgaaa
    12361 ctggggtaaa gaaccacagg atacgtaatg atgcccatca taggttagtt cgctgtaaac
    12421 ttcatcagag atcactaaca gattatgttc ctcggctact tgtagcaagt gaatcagctc
    12481 ggactgggta tacagagctc cggtgggatt gctggcatta ttcagaatga gtattttact
    12541 ttttgcagaa caagcctttt tcagagcctg tggcgttagt ttccattctt cggcttctgt
    12601 cgtttcaact ggcacggggg tgccgccaaa caggctaacc atggctggat aggaaaccca
    12661 gtagggagaa ggaataatga cttcgtcgtt aggctgcagt aataattgca tgagtaaata
    12721 aatgccaagt tttccaccat ttacaaccag gcattgctca ttttggaatg agcaatcata
    12781 ggatctatac atccattcac agcccatatg acgtaattca ggaattccca ttattggagg
    12841 ataaagggta tgtccttgtt ccatagcttg tgtgacggca tttattatgg agggatgggg
    12901 gggtaatttg ggttctccag cactgagatt atgaattttg attcctgcgt taattttttg
    12961 ttgagccaga gaattgatgg caactgttgc ggaatctttg ggcaagttct ttttttcaca
    13021 atggttcatt atttttggac cttttttgag tttgagataa ttatcttgaa taattgattc
    13081 acgaaatcct gctgcaagtg atattgcctg gataaatagt cataatattc aaacaatttt
    13141 ttttcgcgca ggcggtctat tatttttttt ccttctttag cttttaaggc gccgatttgc
    13201 ttagacaatt cttggcgttg agccaatttt tcaataatgt atgcatcagt ttgttcaatt
    13261 tgttttctta gttcttcaat cgtactcatg atgaactcct taccagatag ggaccttatc
    13321 actctatttt ttaactaata taaaagccgt tttattctga tagctgtcct gaatatttct
    13381 cgctctcatt tgcaaaccat aaagtggtgc acaacgtgcc gacgcaatga cggcggtatg
    13441 gggtggtaat attccttctg ccagatctct tgctgctttg gctgtatcga tccattctat
    13501 ttttttcgca tctttaaatt tgtgtcggag gaattttttg cactgggcaa gaccttgagg
    13561 atgcgaaaca atatgggtga ttttatgatg ttgagtattc ggtttaacca gcaggcattg
    13621 gttaatgtca tgccaaatct catcgatggg agtaaatgaa tatttgccca tagcctgaaa
    13681 tgctggtttt accaaccctc ctaaaaggtt aacaacgggt aagatgccca aatctatagt
    13741 ttcattctca atagcattta aaacgccttc catatccagc aaatgaatga gatggacatt
    13801 aatatctttc tctttggcat aaagtagtgc tgcttcctca gaaaatgatc cgatatctcc
    13861 agaaacaccg aagcgaatcg cttctttttc ttcaacgaga ttcttcataa cgcttccctc
    13921 tctgccagtt ccaacaggaa cgattctgtc ttctcccaac tgaggcaagg atcagtaacg
    13981 gataacccgc ttaaatcaat gttatcggga ttgatagaat ctaatttttg attaccttct
    14041 ttgatgaaac tttctaccat gaatcccttg accagttgtt ttaaatcagg tctgtttttg
    14101 atgctttcca gaatattaaa tgcaatctct ggttgtagct gatgattttt tttcccattc
    14161 accagacagt tgtcatggct aacatcaata atgacagaag ggttcactat ctgatgcatg
    14221 cccatgtagt acatgacttc ctccagatga gaaacagagt aattgggagc ttggtttgaa
    14281 ccacgcagaa cgagatgagc atgatgatta ccatgggttt gcacttcata gccatcgaag
    14341 acagcgacat gaggatgctg tgatgcaacc aggctgttta ctccgacagc gagtgaacca
    14401 tgtgtagggt tttttaatcc tacggcacag tcaagggcgg aggcaaaaat tcgatgctct
    14461 tgatcctctg aactacgggc gcctattgca acccaggaca gaagttcaag aaatcctttg
    14521 gcattatggg tgaaaagagc ctcatcagcg atagggagcc ccatttgaat gacttttatc
    14581 atcatgtccc gggtatattt tatacctgcc tcaatatcag gagttgaaaa taaatcgggt
    14641 tgattcaccg gtccagtcca gcctttggtg gttcttggct tttgaatgta aacgcgcatg
    14701 attaatttta aagcgtgttt tactttgctg ttgagttgta ccaaacgctc agcgtattcc
    14761 aataccgctt ttttaggcca tgctgaacaa ggaccaacta tcatcaaaag acgtttatct
    14821 cgcccttcca aaatagcttt gacctcttgt cggtcttgag cgatttgtag ataggcgggg
    14881 tgggatagtg gcaattcccg gataattttt tctattggtg gcagtttttt taagatggaa
    14941 taactcatcg tatcctcctc aagccagaaa gcgctttaga tggatgatga atttgacatt
    15001 gatggttcat cgttcacact cctcttttgt acccaacccc cttattagac tggggatctt
    15061 gggtatcaaa aacccccaga ctcagctggg ggttggagtg ttagtattgc ctacctacca
    15121 cccagctcag ggctgaaaaa gctaaaataa aaaaagcaag caaaagcctg taaaaggctt
    15181 gcttttttat ttaactgatt tggtgtagta gtgtagaaca tgaataatca caaaaaattt
    15241 aatttgtata ttatgtatac aggactatgg taaacctgtc aacacctgtt agcgatagtt
    15301 aattttatag ccaattttcc cctgggaatt aacccaatga tttacatata aaaatcactt
    15361 tgacattaat agaactcact gatctgaaaa tgagatcaaa gtattgcgcg aatcattttg
    15421 atcgtataca ttgcatttta tatcaaataa aaactaaggg attaatgttg tgtcaatatg
    15481 ttgggattta atcataaatc ataataccct gctgcaaacc aatttcactg gtcaccgggt
    15541 tcaaatgaat gactccgaat ccacccctaa aggaattctt ttcgtatcaa ataaactatt
    15601 tatcgattta ctcacttata tcactgagta tcagtccatt accagaaaag aactcatgaa
    15661 tctttcaaat aatgaaatca aaccaaggag gaaaagcaat aagagactat taatgtggtg
    15721 agggttttga atttacaaaa aggagtcaat atgtttaatg tggataatag tggtaaaggc
    15781 aattgcttgt attatgctta cagtatttca ttaatgtatt atttgcgggc aaaaaataat
    15841 gtcaggatta ctgaagatat ttttaacaaa ttaggattaa aggaagaaga tagggcccgt
    15901 ttacgaaaac tattatccaa agaccctgac agggcgttta cccgcgatga aatcaaaaca
    15961 atcatagaac ccattcttgg cagggcaacg agagatttgg cagcggaaca taccaaagtc
    16021 gaattcaaat cttcacctca tgatacgcct ttattttcat ctctccatta tgctgtggag
    16081 tttggtttta aacgctcact acaaatcaat gaatccgagt tgactctgtt aatagacaat
    16141 gatttctcga atcctgatta tactgaggct gaaatataca aggtaagtgg tttgttggat
    16201 gctcttcagg aatatatttt aacaagaaca ccttccgtca tagaagaatt caatcgccaa
    16261 tgggaaaata aaaaacagga actcaaagag caaagcttaa ctgaaaaaga gatccagatt
    16321 catcaggcga ctattttaga taatatccta agaaaagaaa cgatagattt tttattagct
    16381 gataatgaaa aacatcttga tgaatacaga gagcatcttc gaagagagtt cgtatggggt
    16441 agtgaagaaa cgctgatggt tttacatcgc gccatacaag gtgaacgcat ggtacgtaac
    16501 catgagggaa gaattgagcc tgtctatgat catgaaatta ttttgcatgt tcatagaaat
    16561 ggagcaagtc cgtcctatca ggcaggcagt ccggaaatga tattaaataa tgaaggcaat
    16621 gttcattgga cttctatcat tcctgatgca atatttacct caaaactaac agacaaagaa
    16681 gaaaaactac tcgaaatgct ggagcggatg cagtcagagt atggcagcaa agagcttggg
    16741 gtagaaaaat actcttcgat aagtgattgg cttagtgatt tgatgaagca gatagagctt
    16801 attaaaacca gcccaactgc tgtgacaaaa caaaaagaaa ttgaattgtt ttttcagctt
    16861 ctggctaaag caacacctaa attagcatcg gaacctgcct tgaggatgtc gcttggtact
    16921 cttttttcca attttctcga atgtattcca gcccttatgg ttgaaaataa actcagcaca
    16981 ggcttagcta aatttggatt gtatagtcct gtaatatctc agaaaaaaga agatttgtcc
    17041 ttgaccaaat catctgagga gaaagttaat tctgaacagc ctcaaaaggg cgtattaagt
    17101 caattctcga aacatactct gcttaaggta tctgaggatg taagcccaaa accataccct
    17161 acagaactaa acccgtatgt tcgaaaaaac aagcagtctg gtcctcgagt agcgagagca
    17221 ctcacccaaa tgtaccaaaa aggacttgaa ggtgatgaaa gatatacgcc agaaaagtgc
    17281 aaagacattg cgcagaggta tatagggtat tcgaaatctc ataaacgcaa tttcattctt
    17341 gatgatgtca tcctctttgt gcaggaaatg gataaaatca ttaagcaaca agaatcaatt
    17401 agtgaaaact cctctaagcg tgctgcaatg taagggtgct gtttttgccc gaagtgtggg
    17461 aaaaacaaca tcgtcagatt gattttgctt tcttatcaag tggtgactta attggtgagg
    17521 aagcaaaata gcaaccgcaa tgattttcta ggctgtaatt aatataaatg catatagccg
    17581 gtaaatatta ttatttaaat ttaggtggtg ttttctctaa aggcagtttt tcgctcattt
    17641 gcatttcttc aggatgactc tggaaaaaat ggatggactt ttcacttgta gctggcctgg
    17701 atgtaacaat ttggatctct tttttcaatt caggtgatgc gcgaactgcg ccacataaat
    17761 aaagcacttg tggtttaatc tgtttttcag tcgtattatt tcggccattt gccagcatta
    17821 tttctccaag ttctttttct gttaattgga tttgctgaga gggatcggtg ttggtcgcaa
    17881 taacgaatga ggttccagta taaccccaac cctggtgaac aataaaggtt accaaatctg
    17941 gatgattcgg aagaaagacc cgctgcgccc attcggggaa gtgaggattg ggtagctggg
    18001 ccagtgttgt attaatttca gagatatagt ctgacagatc aaatcgtctc agatggtttt
    18061 gttgatgata ttcaacaggg gttggatcaa aaacgtttac tggcttggta gcaccatatt
    18121 catcaccgta ttgtaaaatc caaccgccat cagaacaaaa tgcgctgaca gccagttttt
    18181 cgcgcatgcg ttttgcaagc aatttatcat catggatacc tttttccacc agttgctgaa
    18241 tatagcgtgg ctcatcatga gatccaggaa aaccaattgt aggaccaacc tgctgtaata
    18301 atcctttatt ttgagtaaac caattgtcat cctgttgcca taagttatat gaataaccat
    18361 agccaccttc ggggcccggc atccagaaca cactattcat cgtgtgggtt acaatacctt
    18421 tcaaagcaag attttcttcc atataacctc tgcctatggt ttctgcaata ataagtgggt
    18481 cacgttgaaa tttgtgttgg caagcctcct ttgcgatttc tgttagtcga gacaatacct
    18541 tgttatttac catggctggg gcatcaatac gtattccctg aaaaccaaga tgctcaatat
    18601 tacgctcgat aaatggtttc caataatatt cgatgatttg ttcgcaaatg acagggtcat
    18661 catagttaaa cgttgctaca tcactccagg gattattggc caacggattg tagttttcgt
    18721 ccatgttatg catcaccaaa tttccattgg ggtgacgttt aaaccatttt ttcgtatcaa
    18781 tattgtattt ttcaagaaaa aatttatcgc cattcacaaa acgatgatct accgccaggt
    18841 gtcttgcgac caaatcaaat ataggcatta aaccaagctc atttgctttt gcagtgtatt
    18901 gctgaacgct tttatcattc ttggcatagc gtggataaat tgattcatcc tgcatggcat
    18961 aagggctatg aatacgatta gccatgttag ggacaggatt atattgacaa ggtgtataaa
    19021 atgggttgac ccaaacttgc ttaaaaccca tcttctgaat tcgctgcaaa tcttcccgca
    19081 tcgcatcaaa atcttgatat aagccagggt attggttata gcaacaaatc cgtggagaca
    19141 tgctgacttc tcaatttctg ttcctatgta tatgaagtat agtagaagta ttaaattcga
    19201 ccttgttgta ttattttcaa ttatttaatt cctatgcatg cttagaaaat taaccaaagt
    19261 gtgc
//
LOCUS       DBNCWK010000049        19186 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.049, whole genome shotgun sequence.
ACCESSION   DBNCWK010000049 DBNCWK010000000
VERSION     DBNCWK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19186
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.049"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            694..2628
                     /locus_tag="AC06IO_001522"
     CDS             694..2628
                     /locus_tag="AC06IO_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HIO9874716.1"
                     /translation="MHVLDPLTLEEIQESCDILKREKELSDSYRFAWVMTYEPPQQEV
                     LHSSDTDFDRCAFLSIFNKKTNETFEAIVNLNTKKVVEWKQIVLEVPPYGKPPILIED
                     FQKCEQIVKADPTWRAAMEKRGLNDEQIDKIQVDAFSAGYFDEEEEREKRIVKGISFY
                     RDDLKDNGYARPIEGVVAIVDLGNEKVLRVIDDGRNVPIPKEKINYDSDSYPEKRQDL
                     KPLDIIQAEGPSFKVEGWEVNWQNWSFHVGFTPREGLVLHQIKYLDEGKERPIIYRAS
                     VTDMIVPYADPSVSHYWKSAFDAGEYGLGKLANELKLGCDCLGNIYYFDVPTVDDSGN
                     ARLIKNAICMHEEDAGTLWKHYEVRNSTFEVRRARELVVSFFTTIGNYDYGFYWRFGQ
                     DGSLKLEIKLTGIVQTAAIFPDTPYEWGGKLTPELAAPTHQHFFNARLHMMVDGEKNS
                     FSEHEFYPMTMGGKNPYGNAFGSSQKTFEQEGEAARSACAQTQRTWKIFNPNCVNGIG
                     TAPAYKLELPETPLLLADEESYIYKRGGFASKQVWVTQYDPQEKYAAGDYPNQSSGGC
                     GLPAYIKQNRKIDNENLVLWVTLGSTHYPRPEDFPVMPTTTISAKLQPFGFFKRNPAM
                     DLPQGKVQEKGDTCCSSLTY"
     gene            complement(2703..2891)
                     /locus_tag="AC06IO_001523"
     CDS             complement(2703..2891)
                     /locus_tag="AC06IO_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HIO9874717.1"
                     /translation="MADIINLNKKRKAKVRLEKEKKISENRIKFGRTKKEKQQEKQDN
                     ERSERHLDGHKLDKKEEK"
     gene            3552..4421
                     /locus_tag="AC06IO_001524"
     CDS             3552..4421
                     /locus_tag="AC06IO_001524"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HIO9874718.1"
                     /translation="MSDYLDKTASLTPATKRIVCDKATEYPHTGSYNQVATHGTYLCR
                     RCGLALFRGVSQFSSGCGWPSFDDEIANAVARKPDADGQRTEILCARCDAHLGHVFTG
                     EYMTYKNLRHCVNSASLDFIADNEVLDTEEAILAGGCFWGVEYYLKRIPGVLKVESGY
                     TGGHVSEPSYEQVCSGTSGHYEAVRVVYDSDKTSYHDIVKRFFEIHDPTQANGQGPDL
                     GQQYLSAVFYFNQEQRQEAELLIQLLKKRGYQVVTQLLQAQPFWPAEDYHQDYYFKHH
                     KIPYCHKSVDRFG"
     gene            complement(4445..5410)
                     /locus_tag="AC06IO_001525"
     CDS             complement(4445..5410)
                     /locus_tag="AC06IO_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874719.1"
                     /translation="MAFSKSETTVDKFSLDDFPPFSAQDLEMAFSLKKKYRELKKSAY
                     PFTGFVCDYERREKLYDFIYGSCHLSKLFENNFKVQIAYLDKEKSGVRHWSLIELQYS
                     IEKGNNALKILVLDSLGAQLSYNTAQVLANEVGFEDLLEEIEGLSIKTYIPDVKLQHS
                     RAGCATFTMDCASMLSTQDKYENIYDFMDKQPDASSTAKVAKLEGDFPVHCTSLPPRI
                     LRVAQSRAIITDDKVLNSTPLINSKEETFKHSLLKHCSFFEDQPQTAENIKRNERIMK
                     KAIKWRNTIDLEMQRSSSEMLETTCAIDDLEEYASSSVSYKSVLL"
     gene            5606..5866
                     /locus_tag="AC06IO_001526"
     CDS             5606..5866
                     /locus_tag="AC06IO_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874720.1"
                     /translation="MKILVNSSVLAIVLSLSTPLFADDTQWMTEHNLVKGVITFQDDS
                     QIQVYAAQADLDKLQQNLANIQSVEVQPNLTDTTTSPNQQGE"
     gene            complement(5909..6178)
                     /locus_tag="AC06IO_001527"
     CDS             complement(5909..6178)
                     /locus_tag="AC06IO_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIO9874721.1"
                     /translation="MSLGELLLIFIVAIIVFGPSKLPMLATHLGMLVRKMNQLKEHTS
                     SFWQQQLNEIQLLENQRKAEKADRQYQQTDSENKNSDDMENTRPS"
     gene            complement(6175..6360)
                     /gene="tatA"
                     /locus_tag="AC06IO_001528"
     CDS             complement(6175..6360)
                     /gene="tatA"
                     /locus_tag="AC06IO_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIO9874722.1"
                     /translation="MGLSGISPLSLLLILAIIVALFGTSKLKTIGSDLGEAIKNFRKA
                     MNSEETNDTQKDDHKPS"
     gene            complement(6362..8011)
                     /gene="ubiB"
                     /locus_tag="AC06IO_001529"
     CDS             complement(6362..8011)
                     /gene="ubiB"
                     /locus_tag="AC06IO_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIO9874723.1"
                     /translation="MKSIKQLIRLIHINYILAKNGLDNVVVSIKLFAPLRFIVYLNPW
                     NWLRKEKLTRGEALRKSLEELGPIFIKFGQALSTRPDILPEDIAKELSKLQDKVPPFP
                     SHIAMNIIEQAYKQSAYDVFAQFDPIALASASMAQVHAATLKTGENVVVKILRPNMRR
                     LIEQDLSIMYTIAALADRYWPEGKRFKPKEIVKEFEHTLLDELDLMREAANAAQLRRN
                     FNQSPMLYIPEIYWDYCHHNILVIERIHGIPVTDIASLRDHGIDIKKLAERGVEIFFT
                     QVFRDCFFHADMHPGNIFVSYQNPKDPQYICVDFGIIGTLTDNDKRYLAENLVAFFNR
                     DYKRVAELHVESGWVSRDTPVAEFESSIRTVCEPIFEKPLKDISFGQVVFRLFQVARR
                     FHMEVQPQLILLQKTLLAIEGLGRQLYPELDLWATAKPFLEKWVREQMGPKAFIKRLK
                     QNLPFFTEQLPHMPKLIFDILEMKKDQLLTVHELTRMQSENNNPTIQWKSVGLGVFFS
                     FLSLGVISYFDLLDYNHLTTITIAGSVLAGIFVLINRKIRN"
     gene            complement(8101..8718)
                     /locus_tag="AC06IO_001530"
     CDS             complement(8101..8718)
                     /locus_tag="AC06IO_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiJ"
                     /protein_id="HIO9874724.1"
                     /translation="MLKEYSLKALQTAINQAMKLDEQMPQKLQKLDGKTLEMVITPLN
                     VNFYIRFKGSEMQLLHRIDGRPDTIIHSNPIGLIRLSLLPTSKARSLFNDKIRISGDI
                     ELGQSIKKLFDEIDIDWEGHLAHFTGDVVAHQIGSFVRKGLEFKNQFSTSMQQNITEF
                     LQEELRICPSRNELEDFFAEVDELVLSVDRLQAHINNLMSDDEGN"
     gene            complement(8726..9478)
                     /gene="ubiE"
                     /locus_tag="AC06IO_001531"
     CDS             complement(8726..9478)
                     /gene="ubiE"
                     /locus_tag="AC06IO_001531"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIO9874725.1"
                     /translation="MTNQKQTTHFGFKSVDWNEKEKKVAEVFHSVAKNYDRMNDLMSL
                     GIHHLWKRYTIELSHVRPGQSVLDLAGGSGDLTRLLSQKVGDSGQVILADINAAMLHV
                     GRDRLLDEGLFKNIRYVQGNAQCLPFADNSFHCITMGFGLRNVTDKDEALQSMYRVCK
                     PGGKLMVLEFSTPVFPGLKPVYDWYSFNILPKIGKFVANDEASYQYLAESIRMHPDQE
                     TLKAMIERVGFEDCHYHNLSGGIVALHIAYKY"
     gene            complement(9603..10028)
                     /locus_tag="AC06IO_001532"
     CDS             complement(9603..10028)
                     /locus_tag="AC06IO_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HIO9874726.1"
                     /translation="MFYGDTVQETRQLFFISWNKYQNKKELSPLEKEIAQVILDHPEY
                     HKVIEQIPSFPEHTYYPELGETNPFLHMGLHLAVREQVATDRPQGIHTVYTKLLEKYQ
                     DPMAVEHLIMDQLAECLWLSQKNNRPPDENHYLITLQNL"
     gene            complement(10254..10859)
                     /locus_tag="AC06IO_001533"
     CDS             complement(10254..10859)
                     /locus_tag="AC06IO_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HIO9874727.1"
                     /translation="MLIYFNGLLLGLSLIMALGPQNVFLIKQGARKNHAALSAVICFF
                     CDVILVCGSVAGLHHLLLAHPNLQVWMIWLGSAFLIYYAIKNLRSALSKNKQTVGETH
                     QPHTRMQIILFALGFSLLNPHAIIDTLVIVGSGSSQYPDHKLAFLAGVISASMIWFSS
                     LTMTTRYFSNLLTQNKVWQKIELLSGILMAYIAMKLITSSL"
     gene            complement(10920..11945)
                     /locus_tag="AC06IO_001534"
     CDS             complement(10920..11945)
                     /locus_tag="AC06IO_001534"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIO9874728.1"
                     /translation="MKIFNAMFSKVNGGLEQVFLNYIPALSSQGNQVIPIIHPKAEIR
                     NSCPQDHLITVHNFNQHDFFAIHRLKKLIQIHQPDCIITHSYRAAYLFKKTRTKVPKI
                     AVCHVKGHYDFGTDAIIALTEQMRQDIINSGIEEHRVFTIPNLIHIPDQIQYREPRNN
                     QIPTIGVCARFAAIKGVDVFINALAELKKRGVAFQAKIAGDGKEKEQYIELIRHHGLE
                     HEITLLGWIEDKESFYKNIDIFCLPSREESFGLVVLESMVHSLPMVLTRLSGPLEIAG
                     DSGCALFVPPEDPVSMADGLERLLRDKDLSKQLALKAFQRVQHYSSTKVAPMLHKVLE
                     NICDINQ"
     gene            complement(12011..12364)
                     /locus_tag="AC06IO_001535"
     CDS             complement(12011..12364)
                     /locus_tag="AC06IO_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIO9874729.1"
                     /translation="MSFKPAVELVKLQQNNREVAVIDGQKILIIWHDNQVHAIQSQCP
                     HLKLPLAKGKITESCSIICPFHKSEFDLKTGEAKCWSPWPPVVGNLLGKVSSRKNLKI
                     YPTQIENGQVLVDVD"
     gene            12746..14359
                     /locus_tag="AC06IO_001536"
     CDS             12746..14359
                     /locus_tag="AC06IO_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIO9874730.1"
                     /translation="MGSLVIKKSIIAALVVALGIAGQSVQAESVPDETLQSEKAQTEK
                     VQAEKTETDLFSPVEQIMNPFYPKYPPTKPATGEQAELIKRGEYLAKMGDCIACHTDV
                     EGGTPAYAGGLPINTPFGLFYSPNITPDKETGIGNWTEKDFIRALKDGRDPKGRNYFP
                     VFPYVYFSKITDDDARALYAYFMSIPPVKLKNKSLPFPFGVPGARFALWGWNLLFFFP
                     QENAIEYDPDKSPEWNRGRYIVDTLGHCSMCHTPMNIFGSAKNRYYLTGSFIDGYWAP
                     NITKYGLESATHQEVADVFKAGELINKAGPVAGPMAEVNHNSLSHLTEADRLAIATYL
                     KTVESEEYLGVSPSDLPPTIARGRQVYLKACLICHQDNKMGAPVIGDSASWYTRLKSS
                     GLTGLYRHAIYGYNQMPVKGACVTCSDNDIISAVDYLLNASLTRSQWQDLASGGVKKY
                     PASGKAVYNENCAVCHNEGKLGAPKIGDKVTWKPLIAQNMDVLIENTIRGKNHLPNGG
                     CNHCSTDEVIEAIKYMVSQSKTEGNYSLW"
     gene            14441..15646
                     /gene="coxB"
                     /locus_tag="AC06IO_001537"
     CDS             14441..15646
                     /gene="coxB"
                     /locus_tag="AC06IO_001537"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIO9874731.1"
                     /translation="MLNRLNICKLIALFSALTSAQSLWAAADEWQLNMYKGVTPLSND
                     MYYLHMVAMAVCAVIGVVVFGVMIYSLIHHRKSKGYQPASFHDNPKLEIIWTIIPFLI
                     LVGLAVPATRVLIRMDDTQLSDVTVKVVGYQWKWQYQYLDQGISYFSSLSTPYEQIEN
                     KQKKNQWYLLEVDNPLVVPVNKKIRFLVTSNDVVHSWWVPELGVKRDAIPGFMHEAWA
                     RIEKPGVYRGQCAELCGINHGFMPIVVKAVSEEEFDRWVSEQTKVEDIYAEKSDKPRE
                     QKTMTRDQLMTLGKQKYDLICSACHQPNGTGLPPMYPPLKGSSVAVGKPISRHIELLL
                     NGVPGSAMQSYKDQLTDEEIAAIVTYERNAWGNNTNDLIQPADVAALRHGTDIKPTMV
                     NKAKAGGLQ"
     gene            15643..17259
                     /gene="ctaD"
                     /locus_tag="AC06IO_001538"
     CDS             15643..17259
                     /gene="ctaD"
                     /locus_tag="AC06IO_001538"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIO9874732.1"
                     /translation="MKYAIAHSEDSHEDHGPEQGKGFMGFIKRWLFTTNHKDIGTLYL
                     WLALISFFVAGAMALVIRLELFQPGHRFVDPNFFNQMTTLHGLVMLFGVVMPAFTGMA
                     NWQIPMMIGAPDMALPRLNNWSFWILPFAFCLLFSTMFHAGGGPNFGWTMYAPLSTTY
                     APPSTDFMIFSIHMMGLSSIMGSINIIATILNLRAPGMTLMKMPLFVWTWLITAFLLI
                     AIMPVLAGAVTMMLADRHFGTSFFNAAGGGDPILFQHVFWFFGHPEVYVLVLPAFGVI
                     SEIIPTFSRKPLFGYHFMVYATVSIAVLSFIVWAHHMFTTGIPLGAELFFMYTTMLIA
                     VPTGIKVFNWVSTMFKGAMTFETPMLFAIAFVFLFTIGGFTGLMLALVPADFQYQDSY
                     FVVAHFHYVLVPGVIFALVAATYYWLPKWTGHMYNERLGKWHFWLSVISVNLTFFPMH
                     FLGLAGMPRRIPDYALQFANFNMIVTVAAFAFGLSQLLFLYNVVATVRKRGTNKDVAT
                     SRVWEGSHGLEWTLPSPPPYHTFTTPPEIK"
     gene            17272..17814
                     /locus_tag="AC06IO_001539"
     CDS             17272..17814
                     /locus_tag="AC06IO_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIO9874733.1"
                     /translation="MIEEKKQKKLLTILTLLVLGMFAFGFALVPIYNSLCKNLGINGK
                     TKMEAVAYDEDKAKIAADREVLVEFVATNNSGVPWAFYPKTKKIKVHPGEIVKLAFYA
                     ENKTDHTMTVQAIPSVTPGIAAKYLKKTECFCFTQQTLNGHEAMNMPLLFHLDTDLPK
                     NVNTVTLSYTLFDVTGRSSN"
     gene            17973..18842
                     /locus_tag="AC06IO_001540"
     CDS             17973..18842
                     /locus_tag="AC06IO_001540"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIO9874734.1"
                     /translation="MGAHGTYYIPKPSHWPLVGSIGLTTTLVGAACWLHHDWYGPYVF
                     IAGLCILVAMMFGWFGQVIYENSKGLYDLQVDRSFRWGMCWFIFSEVCFFGAFFGALF
                     FARYWSVPLLGGEMHPITHYTLWPEFKAVWPLLSNPNNQTFAGAHEAMGAWGLAAINT
                     IILLTSGVTITWAHWALKLRKRTQLLTGMSLTIALGVLFLMLQSYEYHEAYTEMNLTL
                     DAGIYGTTFFMLTGFHGLHVTIGTIMLIVILVRCKKGHFTPERHFAFEGVAWYWHFVD
                     VVWLFLFVFVYWL"
     gene            complement(18865..19137)
                     /locus_tag="AC06IO_001541"
     CDS             complement(18865..19137)
                     /locus_tag="AC06IO_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874735.1"
                     /translation="MLIQPIEPVAVTIKMGKNSYECQMVSLSESELALKSYDYFEKGS
                     EVLFFAKYFRGHAVVCTIEFSDYCFTYKMEIVRIQFQPGLLINTRL"
BASE COUNT         5512 a         3653 c         4115 g         5906 t
ORIGIN      
        1 ctttagaacg ctttgcaaaa agagcaagca tcctttcaag tgccggattt tagtgaataa
       61 ccagagtgta ttcacagagt tatccacaga aaatggggat aagtgatcaa tgcaatccct
      121 gcatcaatac ttgataagtc cttgcgggac aagggatttt ataaaaagag tctggtgggg
      181 tctggttatg agattgaaat acggaaatag ttattcacag aaagggagat gcaatttaga
      241 tgttaagcga tttacatcgt aacaaaaaaa aaaataaaaa aacagtcttg acgaagtcga
      301 ttttttgcaa aaactcctgg ttaaaagtta attaaaaaag tggtcggtta ttttatcgat
      361 attgctgacc gactcaatat gactagtgac tggacaatta tttgattcaa attgattaga
      421 atgatataaa gtgcaccttt aatggttgca tcgcctaatg tgagcgccag gagattaaat
      481 aatataccac ctttaaaagg cctgactttt aatttgacaa gcttatgttg tattcactaa
      541 ttaaaaaata ccataccgca caccgcctat ccgactattc ttagcgacat accgcttttg
      601 tatttctgtc taattacttc aacgcgctgt ctctttagct aacgcaaaga gcaatatcgg
      661 tttttttaat tttaatatgt aaaggatgtc aatatgcatg tgttagatcc cttaactctt
      721 gaagaaattc aggaaagttg tgatatttta aaacgagaaa aagaactgtc tgacagttat
      781 cggtttgcct gggtgatgac ttacgaaccg cctcaacaag aagttttgca ttcaagtgat
      841 actgatttcg accgctgcgc ttttctctct atttttaata aaaaaacgaa tgaaaccttt
      901 gaagcaatcg taaacctcaa cactaaaaaa gttgtggaat ggaaacagat tgtcctcgaa
      961 gtgccaccct atggtaagcc gcctatttta attgaggatt ttcaaaaatg tgagcaaatc
     1021 gttaaagccg atccgacctg gagagctgct atggaaaagc gagggcttaa tgatgagcaa
     1081 attgacaaga ttcaagtcga tgcgttttct gcaggctatt tcgatgaaga ggaagagagg
     1141 gaaaaacgta ttgttaaagg gataagtttt taccgtgatg atttaaagga taatggttat
     1201 gcccgtccca ttgagggggt tgttgcgata gttgatctcg gtaatgagaa ggtattgcgt
     1261 gtcatcgatg acggccgcaa cgttcctatc cccaaagaaa aaatcaatta cgattccgac
     1321 tcttatcctg agaaacgcca agacttaaaa cctctggata ttattcaagc ggaagggccc
     1381 agctttaaag tggagggttg ggaagtcaat tggcaaaact ggtcgtttca tgttggattt
     1441 acaccacgcg agggtttggt tttgcaccag attaagtacc ttgatgaagg gaaggaacgc
     1501 ccaattattt accgggccag tgtgacggat atgatagtcc cctatgcgga tccttctgtg
     1561 agtcattact ggaaaagcgc ttttgatgcg ggtgaatatg gtcttggcaa attggcaaat
     1621 gaattaaagc tcggatgtga ttgtttggga aatatttatt attttgatgt tcccacggtg
     1681 gatgactccg gtaatgcgag attgattaaa aatgcgattt gtatgcacga agaagacgcg
     1741 ggcacgctgt ggaagcatta tgaagttcgt aacagtacct tcgaggttcg ccgtgctcgc
     1801 gagctggttg tcagcttttt tactacgata ggtaactatg attatggttt ttattggcgt
     1861 tttggccagg acggcagcct gaaactggag attaaattga caggtattgt gcaaacggca
     1921 gcaatatttc ctgatactcc ttacgaatgg ggcgggaaat tgactcctga gttggcggcc
     1981 cctacgcatc aacatttttt caatgcccgt cttcatatga tggttgatgg tgaaaagaac
     2041 tcatttagcg aacacgagtt ttatcctatg accatgggtg gaaaaaatcc ttatggcaat
     2101 gcctttggca gcagccagaa aacatttgaa caggaaggtg aggcagcccg atcggcttgt
     2161 gcgcaaaccc agagaacctg gaagatattc aatcccaatt gcgtcaatgg cattggcact
     2221 gcgccagctt ataaactgga attgccggaa acccccttac tgctggcaga tgaagagagt
     2281 tatatataca agcgcggggg atttgccagt aaacaggtgt gggtaacaca atatgaccct
     2341 caagaaaaat acgcggcagg agattacccc aatcagagtt ccggggggtg tggtttgcct
     2401 gcgtacatca aacagaaccg taaaattgac aatgaaaatt tggtcttgtg ggtaacgctg
     2461 ggctctacgc attatccaag accggaagat tttcctgtca tgcctaccac gaccatttct
     2521 gccaagctgc agccttttgg ttttttcaag agaaatccag caatggatct tcctcaaggc
     2581 aaagtacagg aaaaagggga cacctgttgt tcttcattaa cttattaata ggttggaacc
     2641 aagcaccttc ttgaggagag ggtgctttcc tgttggttct taaacttcaa agtctcttgt
     2701 ctttattttt cctctttttt atccagcttg tgaccatcca gatgtcgttc actgcgttca
     2761 ttgtcttgtt tttcttgttg cttttctttt tttgtccttc caaattttat gcgattttca
     2821 gatatttttt tttctttttc caagcgaact ttagcctttc tttttttatt aagattaatg
     2881 atgtctgcca tggcactctc tgttgtaaga ccttgtttat actatagcgc ataaaaccag
     2941 ggtaaatcat ctcaatacac aaatcgatcg aaatggaatg atctgaaagt atacactttg
     3001 agaatgggaa ggactgtcgc aagatagtat tttcagcatt gttttttatg acactttgca
     3061 aaaagagcaa gcatcctttt aagtgctgga ttttagtcta taaccagagt gtattcacag
     3121 agttatccac agaaaatggg gataagtaat caatacaatt cacgtattaa tatttaataa
     3181 atctttacag ggcaaggggt tttataaaaa gggcatggtg tcaaatgcta atgagattaa
     3241 aaataaagac aagttatcca cagaaagtgg gatgctagtg aaggtatggg acgctcatta
     3301 tgataacaaa aaaaaaaata aaaaacagtc ttgactggca caatttttta cctgaaaaag
     3361 atttggatag actgtcatca ggaataatgg caatacaaaa acagctaatc ctgctataat
     3421 ttttgaacaa aaggtgagcc cctgttagca ggattttcac tttaaatcgt tcactgacgg
     3481 cttctgagac tgatggcata gagggtgttc aggagatttt ctcatgagtc gagtgttcaa
     3541 aggagcatga aatgagtgac tacctcgata aaaccgcaag cttaacccca gccactaaaa
     3601 gaatagtgtg cgacaaagct actgaatatc ctcatactgg ctcttataat caagtagcga
     3661 ctcatggcac gtatttatgc aggcgctgtg gcctggcgtt gtttcgtggt gtcagtcaat
     3721 ttagctcggg ctgtggctgg cccagttttg acgatgaaat agctaacgct gtggcaagga
     3781 aaccggatgc ggatggtcag cgcaccgaga tattatgcgc tcgttgtgat gctcacctgg
     3841 ggcatgtgtt cacaggggaa tacatgacgt ataagaattt acgtcattgc gttaattccg
     3901 cttcattgga ttttatcgcg gataatgaag tactggatac agaagaagcc atccttgcag
     3961 gggggtgttt ttggggagtg gaatattatt taaaacgaat tcccggagtg ttaaaagtcg
     4021 agtcgggcta tacaggtggc catgtttcag agccaagtta tgagcaagta tgcagtggta
     4081 cttcagggca ttatgaggct gtgcgcgtcg tctatgattc tgataaaacc agttatcatg
     4141 acattgtaaa gcgttttttt gaaattcacg accctactca ggcgaatggc caaggtcctg
     4201 atttggggca gcaatattta agtgccgtgt tttattttaa tcaggagcaa cgacaggaag
     4261 cagagttatt gatccagcta ttaaagaaaa ggggatatca ggttgtaacc caattattgc
     4321 aagcacaacc cttttggcct gctgaagact atcatcagga ttactatttt aaacatcaca
     4381 aaatccctta ttgccataag tcagtcgacc gttttggcta gttgtttcgg aatatttaat
     4441 ttcttcatag cagtactgat ttgtaagata cgctactact ggcatattct tcgagatcat
     4501 cgattgcgca agttgtttca agcatttctg aactgcttct ctgcatttcc agatctatgg
     4561 tatttctcca tttgatggct tttttcatga ttctttcgtt gcgcttgatg ttctcagctg
     4621 tttggggttg gtcttcaaaa aaactacagt gctttagaag agagtgcttg aaggtttcct
     4681 ctttagaatt aattaatggg gtagaattca gaactttatc atccgtgatg atggcacgac
     4741 tttgtgcaac gcgtaatata cgaggtggta agctggtaca atgcactgga aaatcaccct
     4801 caagttttgc aacttttgct gtgctgctgg cgtctggttg tttatccata aagtcataaa
     4861 tattttcata tttatcctgg gtagataaca tactggcgca atccatagta aaggtagcgc
     4921 accctgcacg tgagtgctgt aatttaacat cagggatgta ggtctttatt gaaagtcctt
     4981 caatttcttc aagcaaatcc tcgaatccaa cttcattcgc taatacctgt gctgtgttat
     5041 aggacaattg tgctcctaaa ctgtcaagta ccaggatttt taaagcatta ttgccttttt
     5101 caatggaata ttgtaactca atgagtgacc aatggcgtac accggatttt tccttatcca
     5161 gataggctat ttgtaccttg aaattgttct caaataattt ggataaatga caactgccat
     5221 aaataaaatc ataaagcttt tcacggcgtt cataatcaca gacaaaaccg gtaaatggat
     5281 aagccgattt ctttaattcc cgatatttct tctttaaact aaaggccatt tccaaatcct
     5341 gagcagaaaa cggagggaag tcatccaaag aaaatttatc gacagttgtt tctgatttgc
     5401 taaaagccat acaataaacc ccattcattt tgtattttta ttatatgaaa tgttccccta
     5461 ttattcaatt gccttctttg tctctcggac agattcatga agttacgact ctatgaaacg
     5521 cattttaata acaaactagt ctataattta tagtgtacct ttttttaagt gcataacgga
     5581 tgtgggtgta tgcaaggagt gcattatgaa aatattagtc aactcatcag tgttggcaat
     5641 cgttttgtca ttgtcaacgc cactattcgc tgatgatacg caatggatga cagagcataa
     5701 tttggtgaaa ggggtcatta cttttcaaga tgattcacaa attcaagttt atgctgctca
     5761 agccgatctt gataaattgc aacagaatct ggccaatatc caatcggttg aagtgcaacc
     5821 caatctaaca gacactacaa cttcaccaaa ccaacagggt gaataagtat tcttttgtcc
     5881 gagttaaatc aatcaagcgc ttgttgattt agctcggacg tgtattttcc atgtcatcag
     5941 aatttttgtt ttcagagtct gtctgttgat attgccgatc agccttttct gccttgcgtt
     6001 ggttttcaag cagttgaatt tcattaagtt gttgctgcca aaaggaggaa gtatgttctt
     6061 ttaattgatt catttttcgt acgagcatgc caaggtgggt tgccagcatg ggaagctttg
     6121 atggcccaaa cacgatgata gcaacaatga aaataagtaa taactcacca agactcatga
     6181 tggtttatga tcgtcctttt gagtgtcatt tgtttcctca ctattcatgg ctttgcggaa
     6241 attcttaatc gcttcgccaa ggtcagagcc tattgttttt aatttggaag tgccaaacaa
     6301 ggctacaata atggctagaa ttaataagag tgataacgga cttattccac ttaatcccat
     6361 gttagttcct tatcttgcgg ttgatgagta caaaaattcc agctaaaaca gaaccagcta
     6421 ttgtgatggt tgtgagatga ttataatcca gcaaatcaaa atagctaatg acgcctaatg
     6481 ataaaaatga gaaaaaaaca cccagtccca cactcttcca ttggatggtc ggattgttat
     6541 tttcagattg catgcgtgtg agttcatgga ctgttaacaa ctgatccttt ttcatctcca
     6601 gtatatcaaa aatcagtttg ggcatatggg ggagttgctc agtaaaaaag ggcaaatttt
     6661 gctttaagcg tttaatgaat gctttaggcc ccatttgttc gcgcacccat ttttcaagga
     6721 atggttttgc tgtagcccat aaatccaatt caggatagag ttgcctgccc aaaccttcta
     6781 ttgccaacag ggttttttgc agcaaaatca gttggggctg cacttccata tgaaaccttc
     6841 tggcaacctg gaataaacgg aaaactactt gcccaaagga aatgtctttt aatggctttt
     6901 caaaaatagg ttcgcaaacg gttcttatgc tgctttcaaa ttctgcaaca ggggtgtcac
     6961 gcgataccca tccggactct acatgcaatt cggctacgcg cttgtagtca cggttaaaaa
     7021 aagcaaccag gttttcagca agataacgct tgtcattatc tgtcagagtc ccaatgatgc
     7081 caaaatcaac acaaatatac tgagggtctt tggggttttg gtaggagaca aagatatttc
     7141 ccgggtgcat gtctgcatga aaaaaacaat cccggaagac ttgagtaaag aaaatttcta
     7201 ccccacgctc ggccagtttt ttaatgtcaa tgccgtgatc tctaagactg gctatatcgg
     7261 tcacgggtat gccatgaatg cgttcaatga ccaggatgtt atgatggcaa taatcccaat
     7321 agatttcagg tatatataac atcggagatt gattgaaatt tcttcgcaat tgagcggcat
     7381 ttgccgcttc tctcattaaa tccagttcat ccagtaaggt atgttcaaat tctttgacta
     7441 tctctttggg tttgaagcgc tttccctcag gccaatagcg gtcagccaat gcggcgatgg
     7501 tgtacatgat acttaaatct tgttctataa gccggcgcat attgggcctt agtattttta
     7561 caacgacatt ttcccctgtc ttcagggttg ccgcatgtac ttgtgccatg gaagcggaag
     7621 ccagggcgat cggatcaaat tgggcaaaaa catcataggc agattgttta taggcctgtt
     7681 caatgatgtt catggcgata tggctgggga atggaggaac tttgtcttgc agtttactta
     7741 attcttttgc gatgtcttca ggaagtatat cggggcgtgt ggaaagggct tgtccgaatt
     7801 taatgaagat ggggccgagt tcctccagcg atttgcgcag cgcttcaccg cgcgttaatt
     7861 tctctttgcg aagccagttc catgggttta aataaactat aaaacgtaaa ggagcaaaca
     7921 gtttgataga cacaacaaca ttatctagac catttttggc taaaatataa ttgatatgta
     7981 taagacgaat aagttgctta attgatttca taacagctca tcggttgttg atttcgagct
     8041 tccagccgct caatatgcaa tgataatcca tccagatcct gaataaaatc atccaatcgt
     8101 ttaattgcct tcatcatcgc tcattaaatt attgatatgg gcttgaaggc gatcaacact
     8161 taacaccaat tcatcaacct cggcaaaaaa atcttccagt tcatttcttg aaggacaaat
     8221 gcgtaattct tcctgaagaa attcggtgat gttttgttgc attgaggtgc tgaattgatt
     8281 tttaaattcc agtcctttac gtacaaaaga gccaatttga tgagccacca catcacctgt
     8341 gaagtgagcc aggtgccctt cccagtcaat atctatttcg tcaaatagtt tctttatact
     8401 ttgccccaat tcaatgtcgc ctgagatgcg tatcttgtca ttaaataaag agcgggcttt
     8461 cgatgtgggt aataagctca agcgaatcaa tcctatagga ttgctgtgaa tgattgtatc
     8521 agggcgccca tcaatgcgat ggagcaattg catttcactc cctttaaagc gaatgtaaaa
     8581 attaacattt aagggggtaa tgaccatttc aagcgttttg ccatccagtt tttgaagttt
     8641 ttgcggcatc tgctcatcga gcttcattgc ctgattgata gctgtttgta gtgctttcag
     8701 cgagtattct tttaacataa tcaactcaat atttgtaggc gatatgcaga gcgactatac
     8761 cgccacttaa attatgataa tgacaatcct caaatccaac ccgttcaatc attgccttta
     8821 atgtttcctg atcaggatgc attctgattg attctgcaag gtattgataa ctggcttcat
     8881 cgttagcaac aaattttccg atttttggca aaatgttaaa tgaataccaa tcataaacag
     8941 gcttaagtcc aggaaaaact ggtgttgaaa attcaagcac cattaatttc ccacctggct
     9001 tgcatactct gtacatggat tgcagcgctt cgtccttatc agtaacattt ctaagtccaa
     9061 accccatagt gatgcaatga aaactgttat ccgcaaaagg gaggcattgc gcattaccct
     9121 gaacataacg aatgttttta aataatccct catcaagcaa gcgatcgcgg ccaacatgga
     9181 gcatggctgc gttgatgtcg gcaagaataa cttgtccgga gtcgcccact ttttgcgata
     9241 ataaccgggt taaatcaccg ctgcccccgg ccaaatccaa tacgctctgc ccaggtcgaa
     9301 catgacttaa ttcaatggta tagcgtttcc agagatgatg aattcccaag gacatcagat
     9361 cattcatgcg atcataattt ttagcaacag aatgaaatac ttctgctact ttcttttctt
     9421 tctcattcca gtctactgat ttaaaaccaa aatgggtggt ttgtttctga ttcgtcatat
     9481 ttgccggata gtatcattaa agtgttcata ttaaccgata atcattaaga tacccagtta
     9541 aaaattgtat actttttttt atccatgtac agatgaaaac aggggtattt ccaggataca
     9601 cgctacaaat tttgcaaggt aattaaatag tggttttcat ctggcggcct attgtttttt
     9661 tgggaaagcc ataagcattc tgccaattga tccataatta aatgttcaac agccatggga
     9721 tcctgatatt tttctaataa cttggtgtaa acagtatgga tgccttgtgg cctatcggtc
     9781 gctacttgtt cgcgaacggc aagatgaagt cccatatgaa ggaagggatt ggtttcgcct
     9841 aattcaggat aataagtatg ttcaggaaaa gatggaattt gttcaatgac tttatggtat
     9901 tccggatgat caagaatcac ttgggcaatt tctttttcca agggagaaag ttctttttta
     9961 ttctggtact tattccagct gataaaaaat agctgtcgag tttcttgtac tgtatcgccg
    10021 taaaacataa tggcccgatt gatataaaat gatccatttt aactgaataa aaaagtaaca
    10081 acaatgttga tgtgcaaata tggtaaaaat gttgacatta atcagtggag tatgagatat
    10141 ttattttgcg aggttggact tgctatgtgt cattccactg ggtcaattgg cgacacactg
    10201 attggccctt tctattatcc tgaaatcctg acaagagcgc tctatgactt aatctataag
    10261 ctgcttgtaa ttaatttcat cgcaatataa gccattaaaa taccgctaag taactctatt
    10321 ttttgccata ctttattttg ggttaatagg tttgaaaaat aacgagtagt catcgttaat
    10381 gaactaaacc aaatcatgct ggcagaaatt acccctgcta aaaaagccag tttatggtca
    10441 ggatattgac tgctgccgct acccacaatc accagggtat ctataatggc gtgaggatta
    10501 agcaggctaa atcccaaggc aaataaaatg atttgcattc ttgtatgagg ctgatgcgtt
    10561 tccccaacgg tttgtttgtt tttagacaat gcgcttctta agttttttat tgcataataa
    10621 atcaaaaagg cagagcctaa ccatatcatc cagacttgca agttcggatg agccaaaagc
    10681 aaatgatgta atccagcgac actaccgcac actaaaatga cgtcacagaa aaagcaaatg
    10741 acggccgaga gagcggcatg attttttcgt gcaccttgtt tgatcagaaa gacattttgc
    10801 gggcctaaag ccattatcaa agataacccc aagagtaatc cattaaaata aatcaacatg
    10861 attgcattca ggacagtaaa aaaaggcgat tatatcataa tattgtgcaa gatgttttat
    10921 tattgattga tgtcacatat attctccaaa actttatgca gcatgggagc gacttttgtg
    10981 ctggagtaat gttgtactct ctgaaacgct ttgagcgcaa gttgttttga caggtctttg
    11041 tctcgcaaga gacgttccag cccatccgcc atgctgacag ggtcttctgg gggaacaaaa
    11101 agagcacagc ctgagtcacc tgcaatttct agtggtcctg ataagcgagt gagaaccatg
    11161 ggcaaggagt gcaccatgct ttccagtaca accaggccaa aagattcttc ccgggaaggc
    11221 aaacagaaaa tatcaatgtt tttataaaat gactctttat cctcaatcca tccaagtaaa
    11281 gtgatttcat gctccaaacc atgatgacga attaattcga tgtattgttc tttctccttg
    11341 ccgtcaccag caatttttgc ttgaaaagca acacctctct tttttagttc cgccaaagcg
    11401 tttataaaga catctactcc tttgattgca gcaaatcggg cacagacgcc aattgtcggg
    11461 atctggttat ttcttggctc cctgtattga atctggtctg gaatatgaat cagatttgga
    11521 atagtaaata cccgatgttc ctctatccct gaattgataa tgtcctgacg catttgttct
    11581 gtgagtgcaa tgatcgcatc agtaccaaaa tcataatggc ctttgacatg gcacacagct
    11641 atcttgggga ctttggtcct ggtttttttg aacaaatagg ctgctctgta actatgggta
    11701 atgatgcaat ccggttgatg gatttgaatt aattttttaa ggcgatgaat tgcaaaaaaa
    11761 tcatgctgat taaaattatg cacagtgatt aaatggtctt gggggcaact gttccttatt
    11821 tctgctttgg gatgaataat tggaatgacc tgattgcctt gagaacttaa agcagggata
    11881 taatttaaaa atacttgttc caaacctcca ttgaccttgg agaacatggc attaaatatt
    11941 ttcactttaa attctcctta tcagccccaa ttctatatta cttaaaggtg ctgccgagtt
    12001 gcaaaatcga ttaatccaca tccaccaata cttgtccatt ttctatctgg gttgggtata
    12061 tttttaaatt ttttctggag gatactttac ccaaaagatt gccaacaaca ggtggccatg
    12121 gggaccagca tttcgcttcg cctgttttta aatcaaattc gcttttatga aaagggcaaa
    12181 taatagaaca ggattccgta atttttcctt tggcgagagg caattttaaa tgagggcact
    12241 gtgactggat ggcatgtact tgattatcat gccagataat cagtattttt tggccatcaa
    12301 taaccgcgac ttctctgtta ttttgctgca atttgactaa ttctacggca ggtttgaaag
    12361 acacgttaat tacctctata ttgaaaatca gaggattata tcagtgatga atttaatctg
    12421 ttagtagaag gctgataaaa taaatcagtt gtgttatcaa aattccttat ttttgctgag
    12481 ttagcccagg gtatcgtatc aatgatcata tctgaatgat gtctttgagc ctgattatga
    12541 agtttcatga aaagatgaaa tttcgagaaa agcttgtggg atataaggca ggtgacagct
    12601 gggttggcat aattataatt tttaaacagg gctgaattta aacggtcgaa taacgttgca
    12661 acttgataag cgaggagtat acaatgcgac aattattctg tctcctcatg agagtcgtga
    12721 aaaataagaa caaataggag ggagcgtggg ctctttggtt ataaaaaaat ctattattgc
    12781 ggcgctagtt gttgcactgg gtattgcagg gcaatctgta caagctgagt ctgtaccaga
    12841 tgaaacgtta caatctgaaa aagcacagac ggaaaaagtt caggccgaaa aaacagaaac
    12901 agatttgttt tctccagtag agcaaatcat gaatcctttt tatcccaaat accccccgac
    12961 caagccagct actggcgagc aagcagagtt gattaaacgt ggtgagtatc tggcaaaaat
    13021 gggtgattgc attgcctgcc ataccgacgt tgaagggggt acccccgctt atgctggtgg
    13081 tttacctatt aatacgcctt ttggcttatt ctatagccct aatattaccc ccgataaaga
    13141 aacaggcata ggaaattgga cagaaaaaga ttttattcgc gctcttaaag atggtcgtga
    13201 tcctaaaggc agaaattatt ttccagtatt cccatatgtt tatttttcca agataactga
    13261 cgatgatgct cgcgctctgt atgcttattt tatgagcatt cctcccgtta agttaaagaa
    13321 caaatcattg ccatttcctt ttggtgtccc tggggctcgc tttgctttat ggggatggaa
    13381 tttgttgttt ttcttccctc aggaaaatgc cattgaatat gatccggata aatcccctga
    13441 atggaatcgt ggaaggtata tagtcgatac cttagggcat tgcagtatgt gccatacacc
    13501 catgaatata tttggatcag caaaaaacag atattatttg actggtagtt ttatcgacgg
    13561 atattgggcc cctaatatca ccaaatatgg attggaatca gcgacgcatc aagaggtggc
    13621 tgatgtattt aaggctggtg aattaattaa taaagcgggt cctgtagctg gacctatggc
    13681 cgaagtgaat cataatagct tgagtcattt gacggaagca gacaggttgg ccattgcaac
    13741 ttatctcaag acagtagaga gtgaagaata cctgggtgtt tctccttctg atttgcctcc
    13801 gaccattgca cgtggaagac aagtgtattt gaaggcctgt ctgatttgtc atcaggacaa
    13861 taaaatgggt gctccagtga ttggtgattc agccagctgg tatacacgtc tgaaaagcag
    13921 cggtttaact ggtttatacc gacatgccat ttatggttat aaccaaatgc cggttaaggg
    13981 tgcttgcgtg acgtgctctg ataatgacat tatttcggca gtcgattatt tgctcaatgc
    14041 atcattgacc cgatcccagt ggcaagattt ggcgtctggc ggtgttaaaa aataccctgc
    14101 cagtggaaag gctgtatata atgaaaactg tgctgtttgc cataatgagg gtaaattagg
    14161 cgctcctaaa ataggcgata aagtgacttg gaagccgctg atagcacaaa atatggatgt
    14221 tttgatcgaa aacacaatca gagggaaaaa tcatttgcca aatggtggtt gcaatcattg
    14281 ttcgacagat gaagtaattg aagcgatcaa atacatggtc agtcaatcta aaaccgaagg
    14341 taattattct ttgtggtagg gttaccagca acatttgaat aaatgaacta gcttaaacat
    14401 tagtcggttt cccggctact tgaagagatg agggattggg atgttaaaca ggttaaatat
    14461 ctgtaaatta attgcgttat ttagtgcctt gacttctgcg cagtcgctgt gggctgctgc
    14521 agatgagtgg cagttaaaca tgtacaaagg ggtgactcct ttaagcaacg atatgtatta
    14581 ccttcatatg gtcgctatgg ctgtttgtgc ggtaatagga gtggttgttt ttggcgtgat
    14641 gatctactca ttaatccatc acagaaagtc aaaaggatat cagccagcaa gttttcatga
    14701 taatccaaaa ttggaaatca tctggactat catcccgttt ttaatccttg tcggtcttgc
    14761 agttccagct acccgagtat taatccgtat ggatgatacg cagctgtccg atgtgacggt
    14821 caaggtggtg ggctatcagt ggaagtggca ataccagtat cttgatcagg gtattagtta
    14881 cttcagtagc ttatcaacac cctatgagca aattgaaaac aagcaaaaga aaaatcagtg
    14941 gtacttgttg gaagtagata atcctttagt cgtaccagtc aacaaaaaaa ttcgattttt
    15001 agtgacttcg aacgatgtag tccactcatg gtgggtgcct gagctgggcg tcaagcgaga
    15061 tgctatcccg ggatttatgc atgaggcatg ggccagaatt gaaaagccgg gtgtctacag
    15121 agggcagtgt gccgagcttt gcggaattaa tcatggtttt atgcctatcg tagtgaaagc
    15181 agttagtgaa gaagaattcg atcgatgggt tagtgagcaa accaaagtag aagacattta
    15241 tgctgaaaag tcggataaac cgcgtgaaca aaaaacaatg acccgtgatc agttaatgac
    15301 gcttggcaag caaaaatacg atctgatttg ctcagcttgt catcagccta atggcactgg
    15361 cttgccaccg atgtaccctc ccctcaaggg tagttctgtt gccgttggaa agccaatttc
    15421 acgacacatt gaattactcc tcaatggtgt tcctggctca gcaatgcagt cttataaaga
    15481 tcaactgact gatgaggaaa tagcagcaat tgttacgtat gagcgtaatg cttggggaaa
    15541 taatactaat gatttaattc aacccgcaga tgttgcggca ctgcgacatg gtaccgatat
    15601 taaacctacc atggttaata aagctaaagc tggaggtttg caatgaaata cgcaatagcg
    15661 cattcagaag attctcatga agatcatggt cccgaacaag gcaaaggctt tatgggattt
    15721 atcaaaaggt ggctatttac aaccaaccac aaagacatag gtactctgta tttatggtta
    15781 gcattgataa gcttctttgt tgcaggagca atggctttgg ttatcaggct tgagttattt
    15841 cagcctggtc atcgattcgt cgatcctaat ttctttaacc aaatgacaac attgcatgga
    15901 ttagtcatgc tgtttggtgt cgtcatgcca gcgtttaccg gtatggcaaa ctggcaaatc
    15961 cccatgatga taggtgctcc cgatatggct ttgccaagac taaacaactg gagcttctgg
    16021 attttaccgt ttgctttttg tctgctgttt tctaccatgt ttcatgccgg tggcggtcca
    16081 aattttggct ggaccatgta cgcgcctctc tctacaactt acgcgccacc cagtactgat
    16141 ttcatgattt tttccattca tatgatggga ctctcatcca tcatgggatc aattaatatc
    16201 atagccacga ttttaaactt gagggctcca ggcatgacct tgatgaagat gcccctattt
    16261 gtctggacct ggcttattac agcgttcctt ctgatcgcca tcatgcctgt tctggcgggt
    16321 gcagttacta tgatgctagc cgatagacac tttggtactt ctttctttaa tgcggcaggt
    16381 ggtggagatc ctatcctgtt tcaacacgtg ttttggttct ttggtcaccc tgaagtgtat
    16441 gttctggtct tgccagcttt tggggttatt tcagaaatca ttcccacttt cagccgtaaa
    16501 ccattatttg gttatcattt tatggtttat gccactgtga gcatcgctgt attatccttt
    16561 atagtttggg ctcaccatat gttcaccacg ggaattcccc tgggggctga attattcttt
    16621 atgtacacca ccatgctgat tgcggtacct accggtatca aggtgttcaa ctgggtgagt
    16681 acaatgttca aaggggccat gacctttgaa acgccaatgc tgtttgccat agcatttgta
    16741 tttttattca ctataggtgg atttacaggc ttaatgttgg cattggtacc ggctgatttt
    16801 caatatcagg acagctattt tgttgtggct cacttccatt atgttcttgt gcccggcgtt
    16861 atctttgctc tggtagcagc gacttattat tggttaccca aatggacagg gcatatgtac
    16921 aatgagcgtt tgggtaaatg gcatttttgg ctttctgtca tttcagttaa tttgaccttt
    16981 ttccccatgc attttctggg gctagctggt atgccaaggc gaatccctga ctatgctttg
    17041 cagtttgcca attttaatat gattgtaaca gtggctgctt ttgctttcgg tttgtcgcaa
    17101 ttgttgttcc tgtacaatgt ggtagccaca gtaagaaaaa gaggcactaa taaggatgta
    17161 gcaacgagca gggtttggga agggtcacat ggattggaat ggactttgcc atctccacca
    17221 ccttaccata cgtttaccac tccacctgag attaaataaa ggtggagttt gatgattgaa
    17281 gaaaaaaaac agaaaaaact gctcaccata ttaacattat tagtcttagg gatgtttgct
    17341 tttggttttg ctctggtccc tatttataat agcctttgca agaatctggg catcaacggc
    17401 aaaaccaaaa tggaggccgt tgcttatgat gaagacaagg caaaaattgc agcagataga
    17461 gaagtcttgg ttgaattcgt agcaaccaac aacagtggtg tgccttgggc cttctatccc
    17521 aagacaaaaa aaattaaagt gcacccagga gaaattgtaa aactggcatt ctatgcggaa
    17581 aataaaacag accatacaat gacagttcag gcaatcccaa gtgtgacacc aggaattgca
    17641 gcaaaatatt taaaaaaaac ggaatgtttt tgttttactc aacaaacgct aaatggccat
    17701 gaggcaatga atatgccttt actattccat ttagatacgg atttaccgaa aaatgtgaat
    17761 acggttactc tgtcttatac attgtttgat gtgacaggta gatcaagtaa ttaggcgtcg
    17821 ttgatatgtc tacctttgcg gctggcttgc ctcgattttc tgaacgttgt ttcttgaaat
    17881 cgtgacaatg caattcacgc tagccaaata tcaacggtct ttgagtatca gctttggctg
    17941 aatgacttat tgttacagga gattaaagca taatgggagc acacggtaca tattatattc
    18001 ccaaacccag tcactggcct ttggttggct caataggctt gaccactaca ctggttggtg
    18061 cagcatgctg gctacatcat gactggtatg gcccttacgt atttatagcg ggcttatgta
    18121 ttttggttgc aatgatgttt ggctggtttg ggcaagtcat ctatgaaaac agcaaaggcc
    18181 tatatgattt gcaagttgat cgttcatttc gctggggtat gtgctggttc atattttctg
    18241 aagtatgctt tttcggagcc tttttcggag ccctgttttt tgcaagatat tggtcagtcc
    18301 ctcttttggg tggtgagatg catcctatta ctcattacac cttatggcct gagttcaaag
    18361 cagtttggcc cttgctgagt aatccaaata atcagacttt tgccggggct catgaagcca
    18421 tgggggcttg gggtttggct gcaataaata ccattatttt attaacttcc ggggtaacca
    18481 tcacctgggc acattgggct ttaaaattaa gaaagcgtac tcaattactg acaggtatgt
    18541 cattgaccat cgctttgggc gttttgttct tgatgttaca aagctatgag tatcatgaag
    18601 catatacgga aatgaattta acgctggatg ctggtattta tggtactacc tttttcatgc
    18661 tgacaggatt ccatggtttg cacgtaacaa tcggaacaat catgcttatc gttattctgg
    18721 ttcgttgcaa gaagggtcat tttactcctg aaaggcattt tgctttcgaa ggggttgcct
    18781 ggtattggca ctttgtggat gtggtgtggt tgttcctatt tgtctttgtc tattggctat
    18841 agagttaacc taattcatcc tttattacaa ccgggtgttg ataagcaagc ccggttgaaa
    18901 ttgaatacga acaatttcca ttttgtaagt aaagcagtaa tctgaaaatt ctattgtgca
    18961 tacaacggca tgacctcgaa aatattttgc aaaaaaaaga acctcactcc ctttttcaaa
    19021 ataatcataa ctttttaatg ctaattcgga ttcagacaag cttaccattt ggcattcata
    19081 gctatttttt cccatcttaa tggttacagc cactggttct ataggttgaa ttagcacagt
    19141 tcccccttaa ctgagagact tgatttgctt agcttaatag tagaca
//
LOCUS       DBNCWK010000050        19054 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.050, whole genome shotgun sequence.
ACCESSION   DBNCWK010000050 DBNCWK010000000
VERSION     DBNCWK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19054
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.050"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            295..1491
                     /gene="lapA"
                     /locus_tag="AC06IO_001542"
     CDS             295..1491
                     /gene="lapA"
                     /locus_tag="AC06IO_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase LapA"
                     /protein_id="HIO9874736.1"
                     /translation="MLEYLTWITANIVLVSNSIFGTPHPVHAQLQVPQCLAAKMTTAH
                     DVLAENEQFKIIDLPGSDIENVTLLAEQVNCGDFIDVTDKFSDTLLTAKRKSAKNILQ
                     SSALTALKKSQEDKNIYEIKHQKEVNAAIKEVVADNIWQTLNHLTSYSNRSATKDTGA
                     AAANWLKSEFETMAMEYNRRDTAAFLVKAGWYKQPSLVTVIGKELKTPAIVIGAPMDT
                     LDGNMPGADEGSGAAAIMESTRILLASKLKFKHPIYIIWYAASDRNLAGSQYVVQYFQ
                     EKSIPVKAVLQLNKTGFRANSDDSTMWVFTDNTNNDLNQYIAKLIKNYIHTPVNFSQC
                     NYECGDHISWSQENIPVTYAVESDFKNLNPYIGSSSDTMDFLNLEHIANFSKLAIAFS
                     IELGSK"
     gene            complement(1549..3228)
                     /locus_tag="AC06IO_001543"
     CDS             complement(1549..3228)
                     /locus_tag="AC06IO_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIO9874737.1"
                     /translation="MCSIGEYLAKRLEELNISEYFAIPGDYNLILLDEVLKNEKLKMI
                     NCCNELNAGYAADGYARVKGVSALFVTYSVGGLSVVNAVAGAYAENLPILVISGGPNT
                     NSIQDAEILHHTLATEDNLYVRDIFSRITAHVVCIRKPEQAAMQIDEAIAIAIAKKKP
                     VYIEIACNIANYPISKPTQRAFNTYKISDSSSLAAAVEHAAEKLNSALKPSLIVGSKV
                     RHCNAINSVSELAKCTGYAMAAMPDAKGFISEQHPNFIGIYWGPVSSSGCAEIIDSSD
                     AYLLIGPNENDYTTVGYVWGINPHKSIKTTKGGVIIGETVYTNIFMNDFLRELSKKLK
                     FNDASYKAYKRIAEETVVYPELDQPDAPLSSRYLFGQIQKMLSDKKALLAETGDSWFN
                     CMRLSLPEGCPFEIQMQYGSIGWSVGALLGMQAALHNQKRVIACIGDGSFQMTAQEIS
                     TMIRYGYKPIIFLMNNACYTIEVQIHDGPYNVINNWRYAELVNVFRGEQSNAKSFIVK
                     TNKELLTAIKQAEKTEALCFIEVFLDKDDCNKNLLEWGSRVANYNSRPPRR"
     gene            complement(3464..4546)
                     /gene="ravQ"
                     /locus_tag="AC06IO_001544"
     CDS             complement(3464..4546)
                     /gene="ravQ"
                     /locus_tag="AC06IO_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavQ"
                     /protein_id="HIO9874738.1"
                     /translation="MMRMFSVRRNPSAVMRKNLSLTSRPLLQAPLLKSTVNPQNTLKT
                     KELKERKFTETLPDSRFFSKLKLAEQDQFSHRKRKRFNYQKNFSAALSLPYSRKELDV
                     NQAEENKLLSMVKQYVIEHANRRVDLFFYTLIAYYNTEIVPTNGYTDLQHGRGRNTNR
                     GRTITEACHSSLTPSLLDSTIYQNKGRLKHKSLLSGTHFMDSLNATVELPPFVNDFDD
                     ILEKVCRKKCLKILHEVSLGRINPMEGLTLFLKMMQDILIKFKEEAASENQSVLLQNS
                     LIKKKFISPNLVDLVINGTLLTTFSDSTQSASDDYIQLLLRMTSEEKALCEKDEKGKI
                     KLYLAKIMDIQNEILTSKNGEFVPDL"
     gene            5033..6520
                     /locus_tag="AC06IO_001545"
     CDS             5033..6520
                     /locus_tag="AC06IO_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9874739.1"
                     /translation="MQNKLLQFICIKKLCKKKKSYKSIIEFASLLLAKKKIICAFTLV
                     SIIKSSWAAHHYDTIIIGAGVSGLTAAYHLHNAQQKVLIIEAKNRLGGRVYTSYDWGF
                     ATDLGASWIHAIENNPLIPLIGKQSIIINTYSNSDPVAMLNNYALYDNEGKPVSKQTQ
                     TLFSSLTKEFLRYCQTRSQMISFAQNLTSFAKQKKLTSEQLALLSYALENIYTYEFAD
                     NLTKLSRNVHSASEASIASGKNALLPEGYFQLFRPLTQHVPIHLNQIVSQINYGPDGV
                     NIITQHEKYHANQVIITVPLGVLKANAIKFHPALPKDKRTAISQLGMGSYEKLYLLFD
                     KVFWDKDKEWIGMLPQNEQEAFNIFNYYKYTKKPVLIVFTSGKLARDMEKEHLTEWVM
                     QHLRRIYGNNIPKPIKNKKTHWGSDPFTRGSYSYLPVNVDKSVIGTLAQPVANRLYFA
                     GEATSTTDPSTVHGAYLSGIRAAEEVLASIKHSVKNRERDKAVNS"
     gene            6546..7370
                     /locus_tag="AC06IO_001546"
     CDS             6546..7370
                     /locus_tag="AC06IO_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874740.1"
                     /translation="MYTKRDNHLLINKEEEQEENQKTVFVPQTNSLALPETQSYFCTA
                     EQCSLNNPYAPAQGYTRPDSLHFKTTPINSEIYEQAFKVSEETVKTETQFANRAPGHL
                     EKEENRSGDWSGFIPDELYQMMMEAAKQGIIVSFLLTLTDEIINDYLKNHHYSQQQIN
                     WISQGIRALTLIAIGTSPGIALGLPISTLLLTEQLGFDKTNVNYLTTSSVVAWGLLSS
                     PFNLLNTSLVMASSIGASVLTSKITQSSCQFVRNRFFSSKSEKEELEHEHILQKTI"
     gene            7683..8993
                     /locus_tag="AC06IO_001547"
     CDS             7683..8993
                     /locus_tag="AC06IO_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874741.1"
                     /translation="MKINIRGVDKADILAALYNGAHGGLAGGFSYLQREMTPQQAKLL
                     IEKQTNLYFDYVDNRPLKIDLSGDVIDATLYDHYNGGCAAQRALSHLTLPSDQPVEEI
                     KKQDPREVFDHLFRSYIRTMDGYPADAYGDNLRALQYAHKACGVNRYFFRDFLNREYT
                     EEGGGLFGLGLISHYTQRKIILKSDPNAGLEILYACFSFIRDKEDSLLNELLSVENPD
                     GPNSQKIARALVGDLEYLRDSRLRWLSTGRQPALIDDFLKDRRKASIVLTAILRGESA
                     PEALKLIMEHITSGNTTLNDLVVYNNLKILQEKISVIPNECIRLELFNILDESIHKHS
                     YSNVLFAMEQSIKKINSSWNYNNTKEKSEALARLRLNFLNDPSQENYQAFIFEAAKPR
                     KSWIFQAQEGKTSSLIAFNNALSEKTKQIYEGNGDYTFSTGMST"
     gene            9207..10679
                     /locus_tag="AC06IO_001548"
     CDS             9207..10679
                     /locus_tag="AC06IO_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874742.1"
                     /translation="MAHDKTFFSQQAHSSLTLVNYVHHDGIGDFRHLLDFVSFFNNTP
                     VLKEIELICIVINSHKPGNPQSVYIKEQLESMNVAHKILIHDSINGHEDLRQEIQKNR
                     PLRKQLQHTIGIIDIALNTSYKLVIQEFCKNKPPTVTIAEIDLYATYDQPSLASDPEK
                     RIDKSRYQADHVYIMGLGTGKVGLKLTEGLFNSPQKAREALQQISDKRIKNLLLDQID
                     EEPISQETINVFLQNNYFIPGYLQDIDTIFGFMSIFATNHKLNAERKNLIFYLNNYQN
                     LFKKNYYDEDTGRRYSQFTDLLFDKHFPWHNVFANSGIRSITIYNYSGQKPTEEEITL
                     NLEGQQITIITGIMLNDTDYQLLYDIPQHFVAASGDNTLENAINHGLLFLMQYKYQYE
                     TVREIAKIAERIKDTLGFNEEEIREFHAYFGEAHHLISYSNEKSTKARALLQSIDLIR
                     LSSNMRKVCAYLIENENFLHQLPEILQTDLLNISKPGMKQ"
     gene            complement(10756..11820)
                     /locus_tag="AC06IO_001549"
     CDS             complement(10756..11820)
                     /locus_tag="AC06IO_001549"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HIO9874743.1"
                     /translation="MFEINDQRKKEYYTALLSKNTCYEGIFYVGVKTTGVFCRPTCPA
                     RKPKLENCEFFHTAQEALLASYRPCRRCQPLSHPNQVSDLVKHLVDAVEANPEKRWKD
                     SDFAALSVDVATARRQFKKRFGMTFVAYARARRMGMAMKQIREGDLVINAQIDAGYHS
                     GSGFRDAFAKIMGAAPSHFNEQHKVLKASWLDTPLGPMLAVSDESALYLLEFVDRRGL
                     EREVERLRNKTKAVIIPGDSPVLKAIQKELELYFSGKLRQFKTPIHLIGSPFQKKAWL
                     ALIDIPYGETRSYLQQAQVIGNPSAYRAVANANGANQLAIVVPCHRIINANGELGGYG
                     GGVNRKHWLLEHERRHDRGC"
     gene            complement(11923..12705)
                     /locus_tag="AC06IO_001550"
     CDS             complement(11923..12705)
                     /locus_tag="AC06IO_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874744.1"
                     /translation="MTLQKYFPQFNEAWQSYSLCRSSLWWPLSYLLLTPLAVFIVFGL
                     IIPLGLNQLGWVSFDHALINYPYLLLVVGLLYLAFFPFLDGLYQMIKAHLDGKPINMN
                     TEDIDFINPGTSKVMGMSMGPILLTIIIMENVGSVFKPFQVLVLLAYVLAIFTPILTV
                     NNPQSPLKKGFECIQFALANKKLVSKVLGLRLLILLSLFFPWIIIGITGEHVILKVIT
                     IMAGVPLFFYSLVKLLPFYFFYPAYVYHQAKHQNRHENISHG"
     gene            complement(12898..14412)
                     /locus_tag="AC06IO_001551"
     CDS             complement(12898..14412)
                     /locus_tag="AC06IO_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1148 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874745.1"
                     /translation="MYMDFGSDNMALSKEIQNKSLRSLIQKYARQLNETDKKLIELIN
                     NLAAADDIPLYAQNQNVINLLIKTIKDSQSEHSPDLEGTSYFIYGLITAEMEAQSEIN
                     RQRHLMQGRFDETALHLRTERDQMQDHDMTLLMPKSQGRIVVMAVLNRYDSHSANAII
                     ETLASDVFNPEVHYIMIPVGPGHWRGVYLSKPQGGTSDTAYDLELFDPYGPEGAAVLD
                     DYVLDLLNQCGVPKELVNIRHTGPKHPQGDAYSCGDFTCAYSHKKMKEFGAPEGSYNP
                     ILIDTLDNLGNVDNVLRMATREETRVLVEKDRGRQHIHPAVQSMRAADKKEAISSTGK
                     LKTGAKGISFEIENANKSKKATIHQTVKESQLSPAMRTGLIIGGGTFTSGGVGAIIGG
                     LMGFFLLPGLGVFMGAIAGAFIGAGAGLLISGIIANAFWNSTPLNIIKTHESNTEIQG
                     VDLKSDSAGKPDKECSEKDEKPIHSPPLFSTPTSRERVTKDMADELSSNGPKTG"
     gene            complement(14705..15208)
                     /locus_tag="AC06IO_001552"
     CDS             complement(14705..15208)
                     /locus_tag="AC06IO_001552"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9874746.1"
                     /translation="MLLFSSAMSKHKLTFTKLSNCMKYLDLAATWAEEEWGYIRNKGV
                     EFRKTILSDLKEHTYIGTFNGQPIAMFVLFDKDMSPEFNYGRLKPPVVCELMYVYIDE
                     PYRGLGFGKQVIDEAKQLASHSKAEYIMLDTLKPGLNRFYEKSGGKVIAENQLFMHPT
                     DVLTIKT"
     gene            complement(15269..16750)
                     /locus_tag="AC06IO_001553"
     CDS             complement(15269..16750)
                     /locus_tag="AC06IO_001553"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIO9874747.1"
                     /translation="MDPYQQLEQHFKKYYDFENLAAIVSWDEATMMPAGGGQARAEAL
                     ATLSAIQHEWLTNKKVGDWIKQAKDLPGLSLWQRRNIYWIDKQYQQATCIPAQLIEEF
                     TNESLICVQAWRELRAKNDWRSFKPYFKKLFEKVKMIADIKSQRFNKPCLDVLIDEYS
                     PDLDCKAITPVFDILKQEIPAIIPNVIEKQKRRDVKVISGDFSAEKQKQFGIEIMSRL
                     GFDFNRGRLDVSHHPFCGGISDDVRMTTRYSEQEFISSLMAITHETGHALYEQGLLNE
                     WSRQPVGHSLGMSVHESQSLLIEMQICRSLEFISFLVPIAKKYFKEAQNITEENLYFH
                     CTKVQPDLIRVEADEVTYPLHVILRYEIEQLLFSSEITIDDLPEVWDNFMRKYLGLST
                     KGNDKNGVMQDVHWPSGNFGYFPAYTFGALIAAQLFSAAKKAIPDINDQIKSGDFQSL
                     LIWLRENVHQKAKLFSYQELLRQATGEELNPQYYLTHIRQRYL"
     gene            complement(16861..>19054)
                     /locus_tag="AC06IO_001554"
     CDS             complement(16861..>19054)
                     /locus_tag="AC06IO_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874748.1"
                     /translation="IANGLAIFKEKFESGSLFIQRKDNHDSFTHEQILQYYLELALTC
                     GRLDIIKYLIDELHVSLSEEKIFHFLFLHSPYEGNKYSHNIVEIYNYLKNEKEIFSEK
                     ALIEEIFLAEAKKGHIQAVKAIIGLKLLPNINITDEAGHTAFFYCILESKKKRFSFNP
                     EELGAVDVILSVRDKEILELIELVDATILAMEDKNEEKLVKLRKVFTDSIDKIPEALI
                     EDCLSRAIELNDVKMIQILLPVCNPDLKFLMLGKVNSVEAGMLLIEHFISTGFVIPEA
                     RSLEGYAHRLGLNEYSAGAELANRFLLHAVAGFVNRTDNGKDFNTILRALQKTVLLNG
                     IAKVEVARKILYQTYLEGAPLLLTVGWTQHGVGLSILWDKESNKTYLVFSNRGQGGIA
                     SCLNAKGLIKKEEPKAGSVIYEIDGKLDEGFFTRFEIGENKLGLFRYGKKAFEKDIRT
                     CLKDARVLPRFIFAAAQNYATCGYVNTKQSIRGLLCVFAMTSGDSLSEELIDRMSRQY
                     KEFTAHDRNVACSELIEDFQRKIKFDSRHIQKEYQDFIVKLILSHCTSDKPEDIERTQ
                     RLFVVLPEEIQEKLAHLIPYHIVTQKADKSSTLQNLSLFRHHEESSTQNDSFLYSPVT
                     VIADSVDNLLDKYHEMNTLLKFLESQGYTEKDIPKIVLENNSVRIHTKPDRDFKYEVK
                     DLFPKDIQVINVQGRYEFILKDLKEVNYVLNNLKSKTSDNQTDLDLGM"
BASE COUNT         6114 a         3811 c         3227 g         5902 t
ORIGIN      
        1 attcagcatg aaaataaaac cagcaaatga gcttatgatt gccttatcag ataaaataaa
       61 ctacatattt cccaactaaa ggctgagaaa tttttcaaga ataaaggtgt taattttgct
      121 aattttgtag tatatttgtc cacgctattt tggagataaa acacatgaca agtaaattgg
      181 tcatttacaa tatacagaaa aaataacgaa ctctccaaaa ttatttttta atacttggaa
      241 taatgtgctt tgcctatgaa tcgatacgga ttctacagtt gtttaggagt ataaatgctt
      301 gaatatttga catggattac tgcaaatatt gtattagtga gtaattcaat tttcggaaca
      361 ccacatccag ttcatgctca attacaagta cctcaatgcc ttgctgcaaa aatgacgact
      421 gcgcatgatg tcctggctga aaatgagcaa ttcaaaatca ttgatctgcc tggtagcgat
      481 attgagaatg taactctttt agcagaacaa gtgaattgtg gcgattttat cgacgtaacg
      541 gataagttct cggataccct gttgaccgcc aaaagaaagt cagcaaaaaa tattctgcaa
      601 agcagcgcat taacagccct taaaaaatcc caagaagaca agaacatcta tgagattaaa
      661 caccaaaaag aagtcaatgc tgcaataaaa gaggtagttg cagacaacat ttggcaaaca
      721 ttaaatcact tgacctccta tagtaatcgt tcagcaacca aagacacagg agctgctgca
      781 gcgaattggt taaagtctga attcgaaaca atggcaatgg agtacaatcg cagagatact
      841 gccgcattcc ttgtgaaagc aggttggtat aaacaaccct ccctggtgac agtcattggt
      901 aaagaactga aaacacccgc catagtcatt ggtgcgccaa tggatacttt agatgggaac
      961 atgcctggcg ctgatgaagg tagcggtgct gctgccatta tggaaagcac tcgtattttg
     1021 ttagcctcga agctgaaatt caagcatccg atttatatta tctggtacgc tgccagcgat
     1081 agaaatttgg cagggtctca atatgtagtc caatattttc aagaaaaatc tattcctgtt
     1141 aaagccgtac tgcaactcaa caagacaggg tttcgcgcta actcagatga ttcaaccatg
     1201 tgggtgttta ccgacaatac aaacaatgat ctgaaccaat atattgcaaa attaataaaa
     1261 aattacatcc atacaccagt taatttttcg cagtgtaatt acgaatgcgg tgatcatatt
     1321 tcctggagtc aagaaaatat acccgtaacc tatgcagttg aatctgactt taaaaacctt
     1381 aacccctata tcggcagctc ttcagataca atggactttt tgaatctgga acatatagct
     1441 aacttttcaa agctggccat tgccttctca atagagctgg gatccaaata aaaactcgtc
     1501 aaatcattaa tatcagggta ggcaattctt ataattccta cccaagatct atctacgagg
     1561 aggacggcta ttataatttg ccactcttga accccattcc aacaagtttt tattgcaatc
     1621 atccttgtcc aggaacactt caataaaaca aagcgcttct gttttttctg cctgtttaat
     1681 tgcagtgagt agttccttgt tggttttaac tataaaagat tttgcattac tctgttcccc
     1741 gcggaataca ttaaccagtt cagcgtaacg ccaattatta atgacattat aaggaccatc
     1801 atgaatttgt acttcaatag tgtaacaagc attattcatt aaaaaaataa ttggtttata
     1861 cccgtaacgt atcatggttg atatttcctg ggcagtcatt tgaaatgagc catctcctat
     1921 gcaggcaatc actctttttt gattatgcaa agcagcctgc atgcctagca aagcacctac
     1981 tgaccaacca atagaaccat actgcatttg aatttcgaaa ggacaacctt caggcaaaga
     2041 caggcgcata caattaaacc aggagtcccc tgtttcagcc aacaaagctt ttttgtcaga
     2101 aagcattttc tgaatttgac caaacaaata acggctgctt aatggagcgt ctggttgatc
     2161 taactctgga taaacgacag tttcttcagc aattcgttta taagctttgt atgaagcatc
     2221 attaaatttt aattttttac ttaattctct gagaaaatca ttcatgaaga tattggtata
     2281 aactgtttca ccaataataa cccctccctt ggtggtcttt attgatttgt gggggttgat
     2341 tccccataca tacccaactg tagtatagtc attctcatta ggcccaataa gcagataggc
     2401 atcactggaa tcgataattt cggcgcatcc agaagagctc actggtcccc aatagatacc
     2461 tataaagtta ggatgctgtt ctgagataaa tcctttcgca tcgggcatag ccgccatagc
     2521 atatccagta cactttgcca actcgctgac agaattgatt gcattacaat gcctaacctt
     2581 actacctaca atcaagctgg gctttagagc ggagtttaat ttttcagcag catgctctac
     2641 tgctgcagcc agagacgaac tgtcgctaat tttataggtg ttgaatgcgc gttgagtagg
     2701 cttggagata ggatagttag caatattaca cgctatttca atataaaccg gctttttttt
     2761 agcgattgcg attgctatag cctcgtctat ctgcatggca gcctgctctg gctttcgaat
     2821 acaaacgaca tgggcagtaa ttcgagaaaa aatgtctctt acatacaaat tatcttcagt
     2881 cgccagcgta tgatggagaa tttcagcatc ctgtatggaa ttagtatttg ggccacccga
     2941 tataaccaag atggggagat tttcggcata tgctcctgca actgcattaa caacactaag
     3001 tccaccaaca ctataagtaa cgaataaagc ggagacgcct tttacccggg catagccatc
     3061 agcagcgtat cctgcattca actcattaca gcaattaatc atttttaatt tttcgttttt
     3121 caggacttca tcgagtaaaa taagattgta atcacccggt atagcaaaat actcagaaat
     3181 atttaattcc tccagccgct ttgccaaata ctctccaatt gagcacatgt taatccctta
     3241 ttaaataaga actttttaaa ttatagcaca gccaaaaatc agtgggtggc aaatgaatac
     3301 ctgctaaaaa atagagttca caaagcatca aattgcaaat gataatttaa atattctatc
     3361 ttggagtatg ttgacaattc gatccagaaa ctatatcccg cgtcttgggc gcgggattaa
     3421 tggatgctgt ggacaaacag gtcggcattt ttgttaacac atcttaaagg tcaggtacaa
     3481 attcaccatt tttcgatgtc aaaatctcat tttgaatgtc cattattttt gccaaataca
     3541 atttgatttt gcccttttca tccttttcac aaagcgcctt ttcttctgat gtcattctaa
     3601 gtaaaagttg gatgtaatca tcagaagcgc tttgtgtgct atccgaaaat gtggttaata
     3661 acgtgccatt gataaccaaa tcaaccaagt ttgggctgat gaattttttc tttatcaatg
     3721 aattctgcaa tagtacagat tgattttcgc ttgctgcttc ttctttaaat tttattaaaa
     3781 tatcctgcat catttttagg aaaagggtta atccttccat tggattgatt ctgccaagtg
     3841 atacttcatg caggattttc aagcattttt tcctgcaaac cttttccagg atatcatcga
     3901 aatcattcac aaagggaggt aattctaccg tggcatttag actgtccatg aagtgagttc
     3961 cggaaagaag acttttatgt tttaatcttc ccttgttttg atagatagta ctatcaagca
     4021 atgatggagt aagactgcta tggcaggcct cagtgatagt tctgcccctg ttggtattac
     4081 gccccctgcc atgctgaaga tcagtataac catttgttgg aacgatttct gtattataat
     4141 aagctatcaa tgtataaaaa aataagtcaa cacgacgatt agcatgttca atgacatact
     4201 gtttcaccat tgacaatagt ttattttctt cagcctgatt tacatctaac tcttttcttg
     4261 agtatggcag tgagagtgcc gcactaaaat tcttttgata attaaatcgc ttacgttttc
     4321 tgtgactaaa ttgatcttgt tctgccaatt ttaattttga aaaaaacctt gaatccggta
     4381 atgtttcagt aaatttacgc tcttttaact ctttagtttt cagagtgttt tgagggttaa
     4441 cagtactctt aagcagcggc gcctggagta aagggcggga agttaaagaa agatttttgc
     4501 gcattactgc ggaagggttt ctacgaacac taaacattct catcatggtc ttctccctta
     4561 atagccatta tgtcaccgta gtacctgctc aaagcatcaa gcgtctgttt ttccataaag
     4621 acacctaaaa cttacttagt gagagttcac agtctggatg atcttcaaag ccagctccct
     4681 gtctaagcca gaacatatac tgtcttttta cagtacataa taatctttaa aaaagcaaac
     4741 attaaaataa cttaatattg aatgcgtcat acagacaagt taattgctat gctgattatt
     4801 atttacaaat attgatactt tttttaatat gcatcaattc aataacaagg atttactgtg
     4861 tcataccaga cttttttctc gacttgatcg ccataattca atcttgtgca ctcgcatgca
     4921 gccaagcaaa tacaaaattc tagccagtcg aggttagatg cgaagcttgg ttgggcaaat
     4981 caatttgtcc ggctaaacct ataaattaaa atagcgatgt aggaagaaaa acatgcaaaa
     5041 caaattatta cagtttattt gcatcaaaaa attgtgcaaa aagaaaaagt cttataaatc
     5101 aataatagaa tttgccagtt tgcttttagc aaagaaaaaa attatctgcg cgttcacatt
     5161 agtctctatt ataaaatcgt cttgggccgc tcatcattat gacacaataa ttattggtgc
     5221 tggagtatcc gggttaactg cagcgtatca tttacataac gcacagcaaa aagtacttat
     5281 tattgaagca aaaaaccgcc ttggaggcag ggtatatacc agctatgatt ggggtttcgc
     5341 cactgatttg ggagcatcct ggattcatgc cattgaaaac aacccattaa taccgttgat
     5401 tggtaaacag tcgattatta tcaatactta cagtaactct gatccagtag ccatgctgaa
     5461 taattatgct ttatatgaca acgaaggaaa gccagtatca aaacaaacac agactttatt
     5521 ttcaagttta accaaggaat tcttaagata ttgccaaact cgcagtcaaa tgatctcttt
     5581 cgcgcaaaat ctcacttcct ttgcaaaaca aaaaaaactt acttctgaac aacttgcctt
     5641 actgagttat gccttggaaa atatctatac ttacgaattt gctgataatt tgactaaatt
     5701 atcacgcaat gtccattcag catccgaggc atccatagct tctggaaaaa atgcccttct
     5761 acctgaaggc tatttccaac ttttccgccc acttactcag catgttccta ttcatttaaa
     5821 ccaaatagtc agccaaatta attacggacc tgatggtgtc aatatcataa ctcaacatga
     5881 aaaatatcat gccaaccaag tcattattac tgtcccatta ggcgtgctca aagccaatgc
     5941 aatcaaattt catcctgctt tacccaaaga taagcgcaca gcaatctctc agttagggat
     6001 gggaagttat gaaaaattgt acttattatt tgacaaagtt ttttgggata aagacaagga
     6061 atggattggc atgctccccc aaaatgagca agaagcattc aatatcttta attattacaa
     6121 gtacacaaaa aagcctgtac tcatcgtatt taccagtgga aaacttgcgc gcgatatgga
     6181 aaaagaacat ttaaccgaat gggtgatgca acatttaaga cgaatttatg gaaacaacat
     6241 cccgaaacca attaaaaata aaaaaactca ttggggcagt gatccattca cacgtggcag
     6301 ttattcctac ttgccggtaa acgttgataa gagtgttatt ggcactctag cccaacctgt
     6361 agctaaccgt ctctattttg ctggagaagc tacctcaacc acagatccca gtactgtgca
     6421 tggcgcttat ctatcaggga tacgcgcagc agaagaagta ctggccagca tcaaacactc
     6481 tgtaaaaaat agagaaagag ataaagctgt caattcataa ccccatcttg attttggaga
     6541 aagtgatgta cactaaaaga gataatcatt tgctgattaa taaagaggaa gaacaagaag
     6601 aaaaccaaaa aacggtattt gtccctcaaa caaatagttt ggctcttcct gaaacccaaa
     6661 gctacttttg tacagcagag caatgctctt taaataaccc ctatgcccca gctcaaggtt
     6721 atacaaggcc tgattcattg catttcaaaa caacccccat aaactcagag atctacgaac
     6781 aagcatttaa agtttcagaa gaaactgtta aaaccgaaac acaatttgca aatagagctc
     6841 ctggacactt ggaaaaagag gaaaatcgtt ccggtgattg gagtgggttt attccggatg
     6901 agttatatca aatgatgatg gaggctgcaa aacaaggcat tattgtctcg ttccttctca
     6961 ctctcactga tgaaataata aatgattatc taaaaaatca tcattattca cagcaacaga
     7021 taaattggat ttctcaaggc attcgagctt taactttaat tgctattggt acttcaccag
     7081 gaatcgcttt gggattaccg ataagcacac ttttgctgac agagcagttg ggctttgata
     7141 aaacgaatgt taattatctc acaacaagtt cagtcgtagc ttgggggcta ttaagctcac
     7201 cttttaattt gcttaatacc tcccttgtga tggcaagttc tattggagcc agtgtattaa
     7261 ccagcaaaat aacacaatca tcctgtcaat ttgtgcgtaa caggtttttt tcttcaaaat
     7321 ctgagaaaga agaactggaa catgagcaca tattgcaaaa aacaatctag cagtaattca
     7381 ctgcaaaatt tttgatgaat tgcttaatac ttttactaat aaactaaatt cggaaggcag
     7441 gcaaatgatt ttcctccaac gagccaaaga tattttaggc tttacgaaga ggaaaatctc
     7501 ctccgccaac ctcatctaaa accatctatc gtgggctatt tcaatgcctt tccttgccca
     7561 ctgagcacta aatacttaag tttaattcat tgacagaata tctttttcac ttgatttact
     7621 cattatctat agattataat ttaaccattt tgtatattta ttaagtttat ataggttact
     7681 ccatgaaaat aaatatccga ggcgtggata aagcagacat actagccgcg ttatataatg
     7741 gcgctcatgg tgggcttgca ggtggtttta gctatttaca gagagagatg acaccgcaac
     7801 aagcaaaatt attaatagag aaacaaacaa atctttattt tgattacgta gataaccgcc
     7861 cattaaaaat tgatctttct ggagatgtca tagatgccac actctatgat cattacaatg
     7921 gaggttgtgc agctcaacgt gccttatctc atcttacatt gccatctgat caacctgttg
     7981 aagaaataaa aaaacaagat cccagggaag tttttgatca tctttttaga tcttatattc
     8041 gaacaatgga tggatatcct gctgatgcct atggagataa cctacgcgct ttgcaatacg
     8101 cacataaagc atgtggagtt aatcgatatt tctttaggga tttcttaaac cgtgaataca
     8161 ctgaggaagg cggtggctta tttggattag gtttgattag ccattatacc cagcgtaaaa
     8221 ttatattgaa atccgatccc aacgctggat tagaaatact gtatgcctgt ttttctttta
     8281 taagagacaa agaagacagt ttattgaatg agcttttatc tgttgaaaac cctgatggac
     8341 ccaattctca aaaaatagca cgtgctttag tcggagattt ggagtatctg cgtgatagtc
     8401 gattgcgctg gttatcaacg ggtagacagc ctgcgcttat cgatgatttt cttaaagata
     8461 gaagaaaagc ttctatcgta ctcactgcca tacttcgggg agaatcagcc ccagaagcat
     8521 taaagcttat tatggagcat attacctcag gcaataccac acttaatgat cttgttgtat
     8581 ataacaacct gaaaatattg caagaaaaga tatccgttat cccaaatgaa tgtattcgat
     8641 tagaactatt taacatactt gatgaatcga ttcacaaaca ctcttacagt aatgtgcttt
     8701 ttgctatgga acaatccatc aaaaaaatca attcaagctg gaattacaat aatacaaaag
     8761 aaaagtccga ggccttagca cgcttacgat taaatttttt aaatgatcct tcacaggaaa
     8821 attatcaggc atttatcttt gaagcagcca aaccacgtaa atcgtggata ttccaggctc
     8881 aagaaggtaa aacttcatcg cttattgcat tcaataacgc tttatccgaa aaaaccaaac
     8941 aaatttatga aggaaatggg gattatactt ttagtaccgg aatgagtact tagaaataat
     9001 cgctattaat taggcagact tttttactac acttaaattt aaacagcaga ttgatactgc
     9061 aacattaccc ccatgcttgg ggcaaaataa tcaaagaaca cttcagattt cataactaaa
     9121 actctcaagc acaggattaa aaggttttga attagctgac tagtattgtc tatatttatt
     9181 attatgatta ctatttattc agaataatgg cacacgataa aaccttcttt tctcagcaag
     9241 cacactctag ccttactttg gttaattatg tgcatcacga cggaattggt gatttcaggc
     9301 atttactcga ttttgtgtct ttttttaata acactcctgt tttaaaagag atagaattga
     9361 tttgtattgt tatcaacagt cacaagccgg gcaatcctca atctgtgtat ataaaagagc
     9421 agttggaaag catgaatgtc gcacacaaaa ttttgatcca cgactccatt aatggtcatg
     9481 aggatttacg acaagaaatt caaaaaaata gaccattacg aaaacaatta cagcatacta
     9541 ttggaatcat tgatattgca ttgaatacct cttataaact ggttattcag gagttttgca
     9601 aaaacaaacc accaacggtc actatagccg aaattgattt atacgctact tatgaccaac
     9661 catctctagc ttcagatcct gaaaaacgta tcgataaatc aagatatcaa gctgatcacg
     9721 tctacataat gggcctggga acaggaaagg taggactaaa attgactgaa ggattattta
     9781 actctcctca aaaagcccga gaagctttgc agcaaatcag tgacaagaga attaaaaatt
     9841 tattgctaga tcaaattgat gaagagccca tttcacaaga aacaataaac gtttttttac
     9901 aaaataatta ttttattcct gggtaccttc aggatatcga tacaatattt ggattcatga
     9961 gtatttttgc cactaatcat aaattaaatg cggaaagaaa aaatctcatt ttttatttaa
    10021 acaattatca aaatctattt aagaaaaatt attatgatga agacacaggg agacgttact
    10081 cacagttcac cgatttattg tttgacaaac atttcccttg gcataacgtt tttgcaaatt
    10141 caggtatccg ctcaattacc atctataact attcaggtca aaagcctact gaagaagaaa
    10201 taaccttaaa tctggaaggt cagcaaataa ctatcattac tggtattatg cttaatgata
    10261 ctgactatca attgctctat gacatcccac aacatttcgt tgcggcttca ggtgacaata
    10321 cgctggaaaa tgctattaat catggtctgc tatttttaat gcaatataag tatcaatatg
    10381 aaaccgttag agagattgct aaaattgcag aacgaattaa agatacccta ggttttaatg
    10441 aagaagaaat tcgagagttt catgcctatt ttggtgaggc tcatcacctt attagttata
    10501 gtaatgaaaa gtccacaaaa gccagagctt tgttacaatc aatagattta atacgtctgt
    10561 cttccaatat gagaaaagtt tgcgcctatt taatagagaa cgagaatttt ttacatcaat
    10621 tacctgagat tttacaaacc gatttattga atataagcaa accaggaatg aaacaataaa
    10681 ttgtatcaat cactacagct tctggtaggt taacatgttt attctatact cctttttgag
    10741 cgtagaaaaa ttagtttagc aaccgcgatc atgtcttctt tcatgttcaa gtaaccaatg
    10801 tttacgattt acaccacctc catatcctcc cagttcgcca ttagcgttga taatccgatg
    10861 acaaggaacc actatagcta actgattagc gccattggca ttagccaccg ctctgtaagc
    10921 cgagggatta cctattactt gtgcctgttg caggtaactt cttgtttcac catagggaat
    10981 atcgattaac gccagccatg cctttttttg aaaagggctg ccaattaaat ggatcggcgt
    11041 tttaaactgt cttagttttc ctgaaaaata aagctccaac tctttttgta tagcctttaa
    11101 gactggggaa tctcccggga taattaccgc tttggtttta tttctcagcc tttcaacctc
    11161 tctttccaat cctcttctat cgacgaattc aagtaaatat aaagcgcttt catctgaaac
    11221 agcaagcata ggacctaaag gtgtatctaa ccaggaagcc ttaagaactt tgtgctgttc
    11281 attaaaatga gatggagcgg ctcccataat cttcgcaaaa gcatctctaa acccgctgcc
    11341 tgaatgatat ccagcatcaa tttgcgcatt aatcactaaa tcaccttctc tgatttgttt
    11401 cattgccata cccattcttc tggcacgagc gtatgctaca aatgtcattc caaatctttt
    11461 cttaaattgg cgccttgctg ttgcaacatc aactgataag gctgcaaaat cactgtcttt
    11521 ccatcttttt tcaggattag cctcaactgc atcaaccaaa tgtttgacca aatcagagac
    11581 ttgatttgga tgtgacagag gttggcaacg tctacagggt ctgtaagagg ctaatagagc
    11641 ctcttgcgcg gtatgaaaaa attcacaatt ttccaatttt ggttttcttg cagggcaagt
    11701 tggtcggcaa aaaacacctg ttgttttcac tccaacataa aaaattcctt catagcaggt
    11761 atttttactc agcagagcag tataatattc cttttttctt tgatcgttaa tttcaaacat
    11821 caccattcct gacaagcttt cttactaatt attataaatc ttctttaaaa tggtgaagcc
    11881 gaaaatcaga cagtgaattt ttaaaattag aaaaggcgcc agttatccat gagaaatatt
    11941 ctcatgcctg ttttgatgtt ttgcctgatg ataaacatag gctggataaa agaagtaaaa
    12001 aggcaataat ttaactaaag aataaaaaaa taaaggcact cccgccatga tggtaataac
    12061 ctttaaaatg acatgctcac cagtaatccc tataataatc cagggaaaga acaaggatag
    12121 aagaatcaaa agacgtaatc ccaaaacctt acttaccaat tttttattgg caagagcaaa
    12181 ttgtatgcat tcgaatccct ttttcaaagg tgattgcggg ttgttgacag ttaaaatagg
    12241 cgtaaaaata gctagcacat aagctaacag caccaatact tgaaaaggtt taaagacaga
    12301 accaacgttc tccatgataa taatagttaa taaaatgggg cccatagaca tgcccattac
    12361 tttggaagta ccagggttaa taaaatcgat atcctcagta ttcatgttga taggcttgcc
    12421 atccagatgg gctttaatca tctggtatag tccatcaaga aaggggaaaa aagccaaata
    12481 aagtaaccct actactagca aaagataagg ataattaatc aaggcatggt caaaactaac
    12541 ccaacccaac tgatttaacc ctaatggaat aattaatcca aaaacaataa atacagcaag
    12601 tggtgttaat agaagatagc ttaaaggcca ccacagcgaa gagcgacaaa gtgagtaaga
    12661 ttgccatgcc tcattgaact gagggaagta tttttgcaga gtcatgaaat gtttcggaca
    12721 gtatttacat taaatgactc atattagcac attaatccca taattaaaag aatacctaca
    12781 aatgctgaat tgaaattttt acagcatgct tcctaatacg tttgtaaaat agaatgagct
    12841 cgactgctat tgtttatttg caggatgcca aagatgaatg aaatatttca ctgattttta
    12901 cccggtttta gggccatttg aactcaactc atcggccatg tcctttgtga ctcgctctcg
    12961 cgaagtggga gtggaaaata aaggaggaga atggataggc ttctcgtcct tttccgaaca
    13021 ttctttatca ggtttacccg cagagtctga ttttaaatcg actccttgaa tctcggtgtt
    13081 actctcatgg gtttttataa tatttaatgg agtactattc caaaatgcat tggctataat
    13141 acctgatatc aacaaacctg ctcctgcacc aataaaagct cccgctatcg cccccatgaa
    13201 tacacccagt cctggcaaaa ggaaaaaacc catgagtccg ccaataatag cccctactcc
    13261 tcctgaggtg aatgtacctc cacctataat aagacctgtt cgcatagccg gtgataattg
    13321 actttcttta acagtttggt gaatagttgc tttttttgat ttattagcat tttcaatttc
    13381 aaaggatatc ccttttgctc ctgttttaag tttgccagtg gacgatattg cctctttttt
    13441 atcggcagcc cgcatagact gaacggcagg atgaatgtgc tgtcttcccc tgtctttttc
    13501 aacaagaact cgcgtttctt cacgtgttgc catgcgaagc acattatcta cattgcctaa
    13561 attgtccagt gtatcgataa gaatcggatt ataactacct tctggagctc caaattcttt
    13621 cattttctta tggctatagg cacaagtaaa atccccacat gagtaagcat caccttgcgg
    13681 atgtttcgga cccgtatgcc gtatattcac aagctctttg ggaacgccac actggtttaa
    13741 taaatcaagt acatagtcat caagtactgc agcgccttca ggtccataag gatcaaataa
    13801 ttcaagatca taagctgtat cgctagtgcc gccttggggt ttgcttaaat aaactcctcg
    13861 ccaatgtccg ggtccaacag gtatcataat gtaatgaact tcaggattga atacatcact
    13921 tgccaaagtt tctataatcg cattagcaga atgagagtcg tagcgattga gaactgccat
    13981 cacaacgata cgcccctgag attttggcat gagcagagtc atgtcatgat cttgcatttg
    14041 atcacgctct gttcttaaat gcaatgctgt ttcatcaaaa cgtccctgca taagatgtct
    14101 ttggcggttt atttcagatt gagcctccat ttctgcagtg atcaatccat aaatgaaata
    14161 agaagttccc tccaaatccg gactgtgttc agactggctg tctttaatgg ttttaattaa
    14221 caaattgata acattttggt tttgagcata caaaggaata tcatctgctg cagcgagatt
    14281 attaataagt tctatgagct tcttgtctgt ttcattaagt tgtctggcgt atttttggat
    14341 taatgatctt aaacttttat tctgaatttc tttacttaat gccatattat cgctcccaaa
    14401 atccatatac agatgctata taaaacaatt aagaaaaaat gaagcctcgt ccctcctctt
    14461 taataatgta tgctccatct gaagcggatc gacaatgctt ttgactcgat aatagacctt
    14521 ttacccaacc tgtctctttt gttttagggc aagaggtcta atgaaataaa aaataattaa
    14581 ccgaaaagaa atttactctc atgattaatt attgaaccca ttgaatatat gattacttaa
    14641 acacgataac atgctcatgt ccatacaaaa aagagcatta cttacccgta atagagatca
    14701 cagcctatgt ctttattgtc aatacatcag ttggatgcat aaacaattga ttttctgcaa
    14761 tcactttccc cccactcttt tcataaaatc gattcaagcc aggttttaag gtatctaaca
    14821 taatgtattc cgcttttgaa tggcttgcta attgtttcgc ctcatcaata acttgctttc
    14881 caaaacccag tcctctgtaa ggttcatcaa tatagacata catcaactca catactaccg
    14941 ggggtttcaa tcttccatag ttaaattccg gactcatgtc tttgtcgaat aaaacgaaca
    15001 tggcaatggg ttgtccatta aaagttccta tataagtatg ttctttcaaa tcagacagga
    15061 ttgttttacg aaactccacc cctttattac gaatataccc ccattcttcc tctgcccatg
    15121 ttgcggcaag atccaaatat ttcatgcaat tagaaagttt agtaaaagtg agtttgtgct
    15181 tggacattgc tgaactaaaa agtaacatct gataaccctc ttaattaaaa tgatacaacc
    15241 tgcaaatttt atccaaggcc accccgtttt ataaatacct ctgtctgata tgagtcaaat
    15301 aatattgcgg gtttaactct tcgcctgttg cttgacgcaa taactcttga taagaaaata
    15361 actttgcttt ttgatgaaca ttttccctaa gccaaattaa caacgattga aagtcacctg
    15421 atttgatttg atcattgata tcagggattg cttttttggc cgccgaaaac aattgagcgg
    15481 caattaatgc accaaatgta taagcaggaa aatagccaaa atttccagaa ggccaatgca
    15541 catcttgcat cacaccattt ttgtcattcc cttttgttga tagtcctaaa tattttctca
    15601 tgaagttgtc ccagacctct ggtaaatcat caatggtaat ctccgaagaa aacaaaagtt
    15661 gttcgatttc gtaacgcagt attacatgta gtggataagt gacttcgtct gcctcaacac
    15721 gaattaaatc aggttgtact ttcgtacaat gaaaataaag attttcttca gttatatttt
    15781 gagcttcttt aaaatatttt ttcgcgatag gaaccaaaaa acttataaac tccaaacttc
    15841 gacaaatttg catctcaata agcaacgatt ggctctcatg cacagacatt cctaatgaat
    15901 ggccaacagg ttgccgactc cattcgttta acaagccttg ctcatacaag gcatgacctg
    15961 tttcatgagt aattgccatt aaggaagata tgaattcctg ctcactgtat cgggttgtca
    16021 ttcgaacatc atctgaaata ccaccacaaa acggatggtg gctaacatct aatcggccac
    16081 gattaaagtc aaaccccaat cgagacatga tttcaatgcc aaattgcttt tgtttttccg
    16141 cagaaaaatc acctgaaatc actttaacat ctcgcctttt ttgtttttcg ataacgttgg
    16201 gaataatagc tggtatttct tgtttcaaga tatcaaaaac cggggtgatg gccttgcaat
    16261 ccaaatccgg ggagtactcg tcgattaaga catccaaaca aggcttatta aatctttgtg
    16321 atttaatatc ggcaatcatt tttacttttt caaataactt tttaaagtac ggtttaaatg
    16381 agcgccaatc atttttagct cgtaattccc gccatgcctg aacacatatc aaagattcat
    16441 tcgtaaactc ttctatcagt tgcgctggaa tacaggttgc ctgctgatat tgcttatcaa
    16501 tccaatagat atttcttcgc tgccataatg ataaaccggg taaatctttt gcctgcttta
    16561 tccagtcacc tactttctta ttggttaacc attcatgttg tatagcactt aatgtggcca
    16621 acgcttctgc acgcgcctgc ccacccccgg ctggcatcat cgttgcttca tcccaggaaa
    16681 caatagctgc taaattttca aaatcgtaat attttttaaa atgttgttct aattgttggt
    16741 aggggtccat gagaaatcct taggtaattc tttaaaatat tgattcctgg tcgcacttaa
    16801 gggctgtatc aaagattcac ataaagcata aaacagttaa accaaatatt ctctcaagtc
    16861 ctacattcca agatctaaat cggtttggtt atctgaagtc ttgcttttta ggttgtttaa
    16921 cacataattt acttctttca aatccttgag aataaattca tatcttccct ggacattaat
    16981 aacctggata tctttgggaa aaagatcttt tacttcatat ttaaaatctc tatccggttt
    17041 tgtatgaata cggactgaat tattttccaa aacaattttt ggaatatctt tttctgtata
    17101 gccctgagac tctaaaaatt taagtagtgt attcatctca tgatatttat ccagtaagtt
    17161 atccacggaa tcagcaatta ctgtaaccgg gctatataaa aaactgtcat tttgggtgga
    17221 agattcttca tgatgtctaa acaggctgag attttgcagc gttgatgact tatcggcttt
    17281 ctgtgttacg atatggtaag gtatcagatg agccagtttt tcctgtatct cttctggtaa
    17341 cactacaaat aatcgttggg ttcgctcaat atcttctggt ttatccgatg tacaatgcga
    17401 taaaatcaat ttgactatga aatcttgata ctccttctga atatgcctgg aatcaaactt
    17461 aattttcctc tgaaaatctt caattaactc ggaacaagca acatttctat catgagctgt
    17521 aaattctttg tattgacgac tcattctatc gattaactct tcacttaaag aatcaccact
    17581 tgtcatagca aaaacacata ataaaccgcg tatggattgt tttgtgttga cgtagccaca
    17641 agttgcataa ttctgtgctg cagcaaaaat aaagcggggt aatactcggg catctttaag
    17701 acatgtcctg atgtcttttt caaatgcttt cttcccatat ctaaaaagac ccaatttatt
    17761 ttcccctatc tcaaaacgag tgaaaaagcc ttcgtccagc ttaccatcta tctcatagat
    17821 aactgaacct gctttcggtt cctctttctt aattaaaccc ttagcattaa gacatgaagc
    17881 aattcctcct tgccctcggt tagaaaatac aagataagtt ttattggact ctttatccca
    17941 taatatggat aaaccaacac catgttgagt ccatcccacc gtaagcaata gaggagcacc
    18001 ttccaaatac gtttgatata aaattttccg cgccacctcc accttcgcta tgccgtttaa
    18061 tagcacagtc ttttgtagcg cccttaaaat agtattgaag tctttaccat tgtctgtccg
    18121 attgacaaat ccagcgactg catgtaacaa aaatctattc gccagttcgg caccagcact
    18181 gtattcgttc agccctaatc tgtgcgcata tccctccaaa ctccttgctt cgggtataac
    18241 aaatccagta cttataaaat gctctattag tagcataccg gcttcaacgg aatttacctt
    18301 acctaacatc aagaacttta gatcaggatt acaaacaggt aacagaattt gaatcatctt
    18361 gacatcattc aactcaattg ctcgcgacaa acagtcttct atcaacgcct caggaatttt
    18421 atctatagag tcagtaaaca ctttccttaa tttaaccagt ttttcttcat ttttatcttc
    18481 cattgccaaa atggttgcat caaccagctc tatcaattca agaatttctt tatcccgaac
    18541 tgataaaata acatccactg ctcccaattc ttcaggatta aaggagaatc tctttttctt
    18601 gctttccaaa atacagtaaa aaaatgctgt atgcccggct tcatcggtaa tattgatatt
    18661 gggtaaaagc ttaagtccta tgatggcttt tacagcctga atatgacctt ttttagcttc
    18721 agccaaaaat atctcttcaa tcagcgcttt ttcgctaaaa atttccttct catttttcaa
    18781 ataattataa atttccacga tgttatgaga atatttattt ccttcataag gactgtgcaa
    18841 aaatagaaaa tgaaatattt tttcctcgga taaggaaaca tgaagttcat caataaggta
    18901 tttaatgata tcaagacgcc cacaagtaag agccaattcc aaataatatt gaaggatttg
    18961 ttcatgagtg aaagagtcat gattgtcttt tctctgtatg aataaacttc cactctcaaa
    19021 cttttcttta aatattgcta atccattggc tatg
//
LOCUS       DBNCWK010000051        19048 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.051, whole genome shotgun sequence.
ACCESSION   DBNCWK010000051 DBNCWK010000000
VERSION     DBNCWK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19048
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.051"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            81..521
                     /locus_tag="AC06IO_001555"
     CDS             81..521
                     /locus_tag="AC06IO_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIO9874749.1"
                     /translation="MKYLHTMVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFL
                     AAPADIEQVQTSYGPMLELTYNWDPEDYTEGRNFGHLAYHVDDIYKICQHLQECGVVI
                     NRPPRDGHMAFIRSPDNISIELLQKGEPLPKQEPWLSAPNIGHW"
     gene            612..1676
                     /locus_tag="AC06IO_001556"
     CDS             612..1676
                     /locus_tag="AC06IO_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874750.1"
                     /translation="MQILKSLSEGMGTAGGVAWTTFGLLFGSLGLTVGNSALTISLGA
                     ISASLFLLVAIPVTYWSYCNSHKENDELRHAIEEQENQIVHYLTEYLLATRQECLLKN
                     PQKYRNHYDKSVLAEDLKNRLLKTRLKRPVSFTTAQFLDCLLDDNHKNSILGMLIDAC
                     TMDKLVLKKKLLTIYRDSVFLQFEELSLFAKLKTGFIAFCAGFGSLAGCSSGSLGLFA
                     ALGIFPGFAAVPLVGLAIMGIACVFGLLMSGMSIDTAIQRSKEKQLFLQSEAIHHDLK
                     NLATLKTASVKAELKAQTKYQIGAKEGLTEKLTMSKLLKRSSSFDSYFYRNHPLWNIS
                     HNLPEASKCGFENLGSPFSL"
     gene            2015..2090
                     /locus_tag="AC06IO_001557"
     tRNA            2015..2090
                     /locus_tag="AC06IO_001557"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2048..2050,aa:Ala,seq:ggc)
     gene            2129..2204
                     /locus_tag="AC06IO_001558"
     tRNA            2129..2204
                     /locus_tag="AC06IO_001558"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2163..2165,aa:Glu,seq:ttc)
     gene            2261..2336
                     /locus_tag="AC06IO_001559"
     tRNA            2261..2336
                     /locus_tag="AC06IO_001559"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2295..2297,aa:Glu,seq:ttc)
     gene            complement(2377..3702)
                     /locus_tag="AC06IO_001560"
     CDS             complement(2377..3702)
                     /locus_tag="AC06IO_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage deoxyguanosine triphosphatase"
                     /protein_id="HIO9874751.1"
                     /translation="MWTHRRSGQTNQRGSQDHRDPYERDRTRVIHCPAFRRLQRKTQI
                     LGTDEGDFHRTRLTHSLEVDSIGRSIVRNLMTNQEYQLLLSSLLPNDDLISVICLLHD
                     IGHPPFGHGGEVALNYMMRHYGGFEGNGQTLRLLTKVENSYGGFGLDLTRRALLGILK
                     YPVKRANVAAPKQPPIHESIHKTIKINDWLPPKAYFDCEQPEVDWLLSPLSDNDRELF
                     QSLSVKPNGTQAGKAAYHNFDCSIMDTADDIAYGVHDLEDAIHLRLINRSHLDTPEFR
                     QLIGETTLSLHQERLLNSLFSPEICLRKQAIGEMVNYFITSTQIIVTNEEFENSLLKH
                     NIALIPEAHALLRYLMQCIYNNVIDSQEARTFEYGGQTVVLRLFDAISSNPASLLDNK
                     NRVLFSQAPDEVAAYRVVCDYLANMTDEYAYRMHERLFGFNTRTIFERL"
     gene            3975..4508
                     /locus_tag="AC06IO_001561"
     CDS             3975..4508
                     /locus_tag="AC06IO_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIO9874752.1"
                     /translation="MMGISIRYIILGIGVMNISSCMVYEDTYTTRYQIYTYDYGQLYP
                     QTDYRARDYNYGNQSGQSVVVPDSYHVGEYHSPVSFKDRDRNWVNSQNPQGYTIQIAD
                     DEKAAYVAQKLYKAPKNDRMAQVKYYQNGKTHYKGLYGTYDSQEAAQKALESLPPEIK
                     QGAGIKDWGSVQGNLNE"
     gene            complement(4614..5084)
                     /gene="lspM"
                     /locus_tag="AC06IO_001562"
                     /gene_synonym="gspM"
     CDS             complement(4614..5084)
                     /gene="lspM"
                     /locus_tag="AC06IO_001562"
                     /gene_synonym="gspM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     LspM"
                     /protein_id="HIO9874753.1"
                     /translation="MKTYLNTLNDREKWMVIVAGVSLFIYGYYLLLYAPLSNQVNQKS
                     TQLIEKTETLEWMKQVRMQKRSAKRKESVDNSQLLTILASQLKNNKTLKFPYQLQQTG
                     SGDVQLTFDAVPFQNFIQWLAKINEVYSINIKQFDVEKTSTPGVTRLMIILSAS"
     gene            complement(5081..6220)
                     /gene="lspL"
                     /locus_tag="AC06IO_001563"
                     /gene_synonym="gspL"
     CDS             complement(5081..6220)
                     /gene="lspL"
                     /locus_tag="AC06IO_001563"
                     /gene_synonym="gspL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspL family type II secretion system protein
                     LspL"
                     /protein_id="HIO9874754.1"
                     /translation="MDKCFIFSKHLDDNGCVCLKISDLGELIAPPEFRNFSQIKDLQK
                     ESSTIIVETCTRAILLSLAIPWLPERKARIAIPYALEDKVTQPVEELHFAFDKFYYQN
                     NQYLVVVIDKQRMKKLIQVFDEHGIEFDAVTLDWFALASQELIISESELLINNEDFKG
                     VLSGDLAQTYLKNHPHNPAHLFQDSKIPVASPVSKNEEHSYTWISKRLIKSKPLNLCQ
                     GEIQHGKTTDWVKKGYLLVGGLCGFWLISILFVNWLSLHWLNRQIDEIDRQTAVIYRE
                     FFPDAKQVISPKFRISQLLGGSTAENQTRFWFILNQLSKAIKDSGITVEQLRYQNKTI
                     SITLVSSDFESLQKIENKLKQSQLKVKQTQASTKDQQVVATLELT"
     gene            complement(6295..7311)
                     /gene="mltB"
                     /locus_tag="AC06IO_001564"
     CDS             complement(6295..7311)
                     /gene="mltB"
                     /locus_tag="AC06IO_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HIO9874755.1"
                     /translation="MRQIIWLGFISLFFLSFTTYSDTTFTKRKDVQSFINMMVTNYHF
                     NRNQLTTVMNQVKLQPQVIESMEKPYEKKNWDVYRELFLTQKRLKSGLQFWAENQKTL
                     EKIQRRYGVSPEIIIAILGVETMYGERQGEYRVLDTLATLAFNYPKRSAFFTKELKEF
                     FLLCREQHVSPTQYKGSYAGAIGKPQFMPSSYRYYAVDFNNNGRRDLVNDNGDSIASI
                     ANYLHKHGWNLNQGIAQPAKISGWRYKQLRMNPKTANYRYSQLISFGVKPITASQNHP
                     PRAGVIELVTRKGKEYWIAYPNFFVITRYNSSPQYALAVYLLSQQLKQHWYTSATKRH
                     RAYV"
     gene            7506..9338
                     /gene="lepA"
                     /locus_tag="AC06IO_001565"
     CDS             7506..9338
                     /gene="lepA"
                     /locus_tag="AC06IO_001565"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIO9874756.1"
                     /translation="MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLT
                     EREMSSQVLDSMDIERERGITIKAQCVSLNYTAKDGKTYLLNFIDTPGHVDFSYEVSR
                     SLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
                     DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGV
                     VSLVRIVNGAIRKGDKMRVMSTGRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKE
                     IQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDA
                     SLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGE
                     TLLIDNPSHLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRH
                     VSVVYDIPMSEVVSDFFDRLKSVSRGYASLDYNFQRFQIADLVKMDILINSERVDALA
                     VIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKNVTAKCY
                     GGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK"
     gene            9446..10201
                     /gene="lepB"
                     /locus_tag="AC06IO_001566"
     CDS             9446..10201
                     /gene="lepB"
                     /locus_tag="AC06IO_001566"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIO9874757.1"
                     /translation="MNFALILVILSAVTGLIALLDWLIWSKKRLPEQKSGRIIEYSRS
                     FFPVFFIVLLLRSFLVEPFRIPSGSLEPTLLVGDFVAVNKFSYGLRLPVWEKKIVPIA
                     NPKTGEIAVFRWPPEPSYDYIKRVIGVPGDKISYHDKKLTINGKEVKQTFVEYTTDES
                     SGKAVTKYKEDLNGVVHDIFVRPDAPAVDFDIVVPEGNYFMMGDNRDDSADSRFWGFV
                     PDSYLRGKAFLVWMSWNGKTDNVRWSRIGRLIH"
     gene            10213..10605
                     /locus_tag="AC06IO_001567"
     CDS             10213..10605
                     /locus_tag="AC06IO_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIO9874758.1"
                     /translation="MRKQQGMTLIGMLFTAVVVIMAGILVMRAAPVYIQHYSILHSIK
                     ALNSTPASSLSGDPLTDVTTLRESVTKRLDINGVEDLKPGELVITPSGPNKFKIKLKY
                     QVIKPLIYNMSLLFNFEDNFEVITGSEN"
     gene            10595..11269
                     /gene="rnc"
                     /locus_tag="AC06IO_001568"
     CDS             10595..11269
                     /gene="rnc"
                     /locus_tag="AC06IO_001568"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIO9874759.1"
                     /translation="MKINLERLCRRLNYHFNNMAYLKQALTHCSAGSDNYERFEFLGD
                     SILSFVIANELFNRFPLHSEGQLSRLRSFLVKGEMLAEIAREIGLGDYLFLGQGELRS
                     GGFRRTSILADALEAILAAIYLDGGMTAAKQIILMLYSSRLDDPDLNHCLKDAKTQLQ
                     EFLQASKFALPEYVLTKVEGDEHAQIFHVTCTIEGVSQVAYGTGPNRRKAEQLAAKAM
                     LEQLQG"
     gene            complement(11297..13147)
                     /gene="abc-f"
                     /locus_tag="AC06IO_001569"
     CDS             complement(11297..13147)
                     /gene="abc-f"
                     /locus_tag="AC06IO_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIO9874760.1"
                     /translation="MLTLRQITLSRGDKILLDKVNVSLYEKQKIGLIGHNGCGKSTLF
                     DFLLGQLSPDTGEYLINPQLSISHLSQHIPDSNEKALDFVLAGDQQYIKLLKRLSEAE
                     KNGDDAEVLQCHEELSHTGGYSKPAQAAVIMSGLGFSGNQQQEPVNSFSGGWRMRLNL
                     ARCLMKPADLLLLDEPTNHLDMEAIFWLERYLKQSPSTIILISHDREFLDAFVTHILH
                     IENRNLTLYSGNYSSFEKIHAQQLALQQAMHEKQQTKIKHMMTFVTRFKAKATKAKQA
                     QSRMKAIEKMEIIAAAQADSPFSFDFFPCPRAGNPLIQCNQVDAGYINHPPILKKIKL
                     SLNPGDRIALLGPNGEGKSTLIKTLTADLSVLSGDIHRSPHLQIGYYAQHQLEQLDCK
                     LSPVETLQTLSPEVREQTIRDFLGGFNFRGDMAVQSIQHFSGGEKARLALARLVWLKP
                     NLLLLDEPTNHLDLGMRSAIELALQTYEGALILISHDRHLLKSSVDNFYLIYQGKVQP
                     FEGDLDDYYLWLQTRNSEKESKLATSPNEYKERKSYQNKLKKLEQLIEHYQNQLRLLD
                     KQLADVSLYEDTKQKNKLDNLLMERDVAHSSLMKTEEEWLEICHALENLE"
     gene            complement(13414..14640)
                     /locus_tag="AC06IO_001570"
     CDS             complement(13414..14640)
                     /locus_tag="AC06IO_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIO9874761.1"
                     /translation="MIMKTKLLPLFDNLNHIHKNHENVILPDTRFHNDFTYCLSFLKS
                     YIGSEGTFNSYRRETERLLQWSWLVKNSTINSLRRDDIEQYIHFCQNPPKTWISLKKV
                     PRFIEKNGKRTPNEEWRPFVVTLSKVSRKNGHLASIDQFKLSQGAVQEIFAILSTLFN
                     FLIAEEYLISNPVALIRQKSKFIRKRQHNAPVRRLSLVQWEAVLRAAESLAEDSPEKH
                     ERTRFILSLLFGMYLRISELAASERWVPSMNDFAKDSNGHWWFTTVGKGNKERQIAVS
                     DAMLESLTRWRLFLGLTPLPSPADNSPLLPKLRGHGPMSNTAPIRRIVQHCFDLAEEQ
                     LRLNGLSEEADNLSAATVHWLRHTGISEDVKIRPREHVRDDAGHSSSATTDRYIDIEK
                     QARYQSARKKIIQPSE"
     gene            14902..14978
                     /locus_tag="AC06IO_001571"
     tRNA            14902..14978
                     /locus_tag="AC06IO_001571"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14936..14938,aa:Pro,seq:tgg)
     gene            14984..15060
                     /locus_tag="AC06IO_001572"
     tRNA            14984..15060
                     /locus_tag="AC06IO_001572"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15018..15020,aa:Arg,seq:tct)
     gene            15222..15297
                     /locus_tag="AC06IO_001573"
     tRNA            15222..15297
                     /locus_tag="AC06IO_001573"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15255..15257,aa:His,seq:gtg)
     gene            15388..15472
                     /locus_tag="AC06IO_001574"
     tRNA            15388..15472
                     /locus_tag="AC06IO_001574"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15422..15424,aa:Leu,seq:tag)
     gene            15502..16833
                     /gene="tig"
                     /locus_tag="AC06IO_001575"
     CDS             15502..16833
                     /gene="tig"
                     /locus_tag="AC06IO_001575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIO9874762.1"
                     /translation="MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGF
                     RPGKVPFNVVKSRFSDSVREEVAREMVQSTLYEALQKNELVPAGYPHVEPLEIEPGKD
                     FKYTAVFEVMPVFEIVELNQAPVELIRSEVTDKDVDNMIEKLREQNKEWHEVTHAVKK
                     GDKVVIDFQGFLDDKPFQGGSAEGYELVIGSGSMIPGFEDGIIGGKIDKPFDIKVSFP
                     EDYGHKDLAGKEATFKITIKKIMEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMAR
                     ELERRVNMMNREKLFDSLMSVNHVELPIALIDKEIEHLKHDMYHRLFGHEHKDDEKIP
                     DFPRELFEEQAKRRVHLGLLFAEYVKKHEIVADNDKVNAMIDKFASAYESPDELRAWY
                     QSSKEHMAEVEALVMEDMVADKIAEDAKLKYKNMDYDSVMNPKKGTEKKGE"
     gene            16836..17480
                     /gene="clpP"
                     /locus_tag="AC06IO_001576"
     CDS             16836..17480
                     /gene="clpP"
                     /locus_tag="AC06IO_001576"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIO9874763.1"
                     /translation="MPGYSDNIIRNASGLIPMVIEQTSRGERSYDIYSRLLKERIIFL
                     LGEVEDHMANLVVAQLLFLESENPEKDISLYINSPGGVVTAGLAIYDTMQFIKPDVST
                     LCIGQAASAAALLLCAGADGKRFCLPNSRVMIHQPLGGYRGQATDIEIHARETLAVRE
                     RLNNIMAKHTKKTPDQIMRDTERDNFMSATQAMEYGLIDKVLYDRQIAGHSTDL"
     gene            17621..18895
                     /gene="clpX"
                     /locus_tag="AC06IO_001577"
     CDS             17621..18895
                     /gene="clpX"
                     /locus_tag="AC06IO_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIO9874764.1"
                     /translation="MSKSGNGNGDKVLYCSFCGKSQHEVKKLIAGPSVFVCDECVELC
                     NDIIREETHETHEETETRLPTPKEISNFLDEYVIGQQHAKKVLSVAVYNHYKRLQHKS
                     EDGVELGKSNILLIGPTGSGKTLLAQTLARILNVPFAMADATTLTEAGYVGEDVENII
                     QKLLQKCDYDVDKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVA
                     SVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVIRERSDKSSIGFSAQLKSKKS
                     SNDEVSKVLGQLESDDLIKYGLIPEFVGRLPVVTTLQELDEAALIDILTRPKNALTKQ
                     FQSLFKMEGSELEFRDEALIAIAKKALERKMGARGLRSILENILLDTMYDLPSLEGVN
                     KVVIDESVVNGLSKPILIYEQDEKKSASGSKD"
BASE COUNT         5646 a         3278 c         4021 g         6103 t
ORIGIN      
        1 tatttgctac cggtatgatg gttatttaaa ctaaatttct gattaataaa aaaataattt
       61 tcaatagtta aggataattg atgaagtatt tacataccat ggttagagtt tcgaatttag
      121 aacaatcgct cgatttttat tgtaataaat tgggtttgat agaagtaaaa cgaactgaaa
      181 atgccaaagg ccgttttacc ttggtatttt tagctgctcc tgctgatata gagcaagttc
      241 aaacttctta tggaccaatg ctagagttga cttataactg ggatccggag gattacactg
      301 agggaagaaa ttttggccat ttggcatatc atgtggatga tatctataaa atctgccaac
      361 atttacagga atgtggtgtt gtaattaata ggccacccag agatggccac atggctttta
      421 ttcgttctcc tgataatatt tctatcgagt tattgcagaa gggcgagcct ttgccaaagc
      481 aggagccttg gttatctgca cccaatattg gacattggta acctgataat tgcattctgg
      541 tagtgaatag tggaaataat ttaatcatac taaatagtat tttgggccaa atggcaatgt
      601 tgaatcaagc gatgcaaatt ttaaaatcac tatcagaggg tatgggaaca gcaggggggg
      661 tggcatggac tacctttgga ctgctatttg gttctttggg actaacggtt ggcaattcgg
      721 cactgactat ttcattgggc gccatttctg ccagtttatt tctattagtc gcaatacctg
      781 ttacttattg gtcttattgt aattcgcata aagaaaatga tgaattacga catgctattg
      841 aagaacagga aaatcaaatc gttcattatt tgacggaata tttattggca accaggcaag
      901 aatgtttact taaaaatcct caaaaatatc gtaatcatta tgataagtct gtgctggccg
      961 aagatcttaa aaatcgatta ttaaaaacca ggttaaaacg gcctgtttcg tttacgacag
     1021 cacaatttct tgactgtcta ttagatgata atcataaaaa ctctattttg ggtatgttaa
     1081 tagatgcctg cacgatggat aagttggtat tgaaaaagaa actattaact atttatcgtg
     1141 atagtgtttt tcttcaattt gaagaacttt ccttatttgc caagttaaaa acgggattta
     1201 ttgcgttttg tgccggattt gggtcgctgg ctggttgttc cagtggttca ttgggtttgt
     1261 ttgcagccct gggtattttc cccggttttg ctgcagttcc tttggttggg ttggcaatta
     1321 tgggtatcgc ttgtgttttt ggtttgttga tgtctggaat gagtattgat accgcaatcc
     1381 aaaggagtaa agaaaaacag ttatttctcc aaagcgaagc aatccatcat gatttaaaaa
     1441 atttggccac gttgaaaact gcatctgtta aggctgagtt aaaagcacaa accaaatatc
     1501 aaattggagc taaagaggga ttaacagaga aattaacaat gtcgaagcta ctgaagcgat
     1561 cttcttcatt tgatagttat ttttatagaa accatccctt atggaacatt tcacacaacc
     1621 ttccagaagc ttcgaaatgc ggctttgaaa atcttggttc tcctttttca ctttgaataa
     1681 cctgtctctt ttattatatt gctgcgttgc aaactttttc tggtactcag ccttgaaaca
     1741 aaaaaccagg ttatgtaaag atctattaca acctttgttt tcgtgctggc caaatatttt
     1801 ttggattaga ctcttacggt cataaatttt tgctgcaatg atgcttttac ccatgttcat
     1861 ttctattgat aatctattga taaaattagc gctgtgattt ggatttattt atgattttga
     1921 tgtaaaaaaa ggttgtctta taaaagactt gcattaaaat agaaaaacca cttgacagta
     1981 ttaatgcgtc taattaaact gcacacctta ttttggggtt atagctcagc tgggagagcg
     2041 cttgcatggc atgcaagagg tcggcggttc gatcccgcct agctccacca tcaacatcgt
     2101 tgtatgttga tggtaaaaaa agtactaggt ccccatcgtc tagaggccta ggacatcgcc
     2161 ctttcacggc ggtaacgggg gttcgaatcc ccctggggac gccattattt ggcatttaaa
     2221 tagtgtagta gtgttttgat gtaatcttaa taagatccag gtccccatcg tctagaggcc
     2281 taggacatcg ccctttcacg gcggtaacgg gggttcgaat ccccctgggg acgccattat
     2341 tttgaatggc tattgtttta aggtttttct tgctatctaa agcctttcaa aaattgttct
     2401 tgtattaaat ccgaataatc tctcatgcat tcgataagca tactcgtcag tcatgtttgc
     2461 taaataatca caaacgactc tataagcagc gacctcatct ggagcttgcg aaaataacac
     2521 tcgattttta ttatcaagca agctggctgg attggagctg atggcatcaa atagccggag
     2581 taccaccgtt tgacctccat actcaaaggt acgtgcttct tgggagtcaa tgacattatt
     2641 atagatacat tgcattaaat agcggaggag ggcatgggct tctgggataa gtgcaatatt
     2701 atgttttaat aaactgtttt caaattcttc attggttaca atgatttgag tagaggtaat
     2761 aaaataattg accatttctc cgatagcttg ttttcttaaa cagatttctg gagaaaatag
     2821 agaattaagc aaacgctcct gatgtagcga gagggttgtt tcaccgatta attgcctgaa
     2881 ctcaggagta tcaagatgtg aacggttaat taatcttaag tgaattgcat cttccaggtc
     2941 atggactcca taggcaatat cgtccgctgt gtccataata gagcaatcaa aattatgata
     3001 ggctgcttta cctgcttggg tgccattggg ctttacggag agagactgaa ataattctct
     3061 gtcattatct gataaaggcg ataataacca atcgacttct ggctgctcgc aatcaaaata
     3121 agctttagga ggcagccagt cattgatttt gattgtttta tgaatcgatt catgaatagg
     3181 gggttgtttg ggagctgcca cattagctcg ttttaccggg tattttaaaa tacctagcag
     3241 cgcacgacga gttagatcca gaccaaaacc accgtaacta ttttcaactt ttgtcagtaa
     3301 gcgaagagtt tgtccattgc cttcaaatcc tccataatgt cgcatcatat aattcaatgc
     3361 gacttctcct ccatgtccga agggcggatg gccaatgtca tgaagcaaac aaatgacgct
     3421 gatgagatca tcatttggca aaaggctgga caataacagc tggtattcct gattggtcat
     3481 caagttgcga acgatacttc tacctataga atctacttcc agggagtggg ttagacgggt
     3541 tctatgaaaa tcgccctcat ctgttccaag aatttgtgtt tttctttgta gtcttctaaa
     3601 tgctggacag tgaattactc ttgtcctatc tcgctcatag ggatctctat gatcttgaga
     3661 gcctctttga ttggtttgac cagaacgtct atgagtccac atatctttgc ccttattcca
     3721 caaactaatt actttattct ataccatgtt gggaaacaat agagtctttg catagtctct
     3781 ggaaaatcac gaatgtttat aatggcattt atttttttga tgcgcgcaga ataacggaaa
     3841 ttttttcgga cgatggtttg aatagaaatc caataactct gaatcaattc ataaaacatt
     3901 ttcctaaagt tttccttgta tttgtagata acaacaaata agagtaaaag taaaagttaa
     3961 aatcaggggg tgttatgatg ggtattagca tcaggtacat cattctaggt atcggcgtaa
     4021 tgaatatttc ttcttgtatg gtttatgagg atacatacac tacccgctat caaatttata
     4081 cctatgatta tggtcagttg tatcctcaaa cagattatcg tgctcgtgat tacaattatg
     4141 gtaatcaatc tggtcaaagt gttgtggttc ctgattcata tcatgttggt gagtatcatt
     4201 ctcctgtatc ctttaaggac agagatagaa attgggtaaa cagccaaaat cctcaaggtt
     4261 atacgattca gattgccgat gacgaaaagg ctgcttatgt ggcccaaaaa ctctataaag
     4321 cacctaaaaa tgatagaatg gctcaagtga aatattatca aaatggcaaa acccattata
     4381 agggtttata tggtacttat gatagtcaag aagcagctca aaaagcattg gaatcattac
     4441 ctcctgaaat taaacagggt gctggaatta aagactgggg gagtgttcaa ggaaatttga
     4501 atgaataggt tatcttgaga aactatatgt atttaaaaaa aatactttga tattctgtac
     4561 tttattatcc taaggtcagg gcttatagtc ttgactaata cataatagca attctaactt
     4621 gcagagagta tgatcatcag tcgggttact cccggtgttg aggttttctc cacatcaaat
     4681 tgttttatat tgatagaata gacttcgttg atttttgcaa gccattgtat aaagttttga
     4741 aaagggactg cgtcaaaggt taattgaaca tcccccgagc ctgtttgttg taattgatag
     4801 gggaatttga gtgttttatt atttttaagt tgggaagcaa gaatagttaa caattggctg
     4861 ttatctacag attcctttct tttggcagag cgtttttgca ttcttacttg cttcatccat
     4921 tccaatgttt cagttttctc aattaattga gtggattttt gatttacctg gttgctcaga
     4981 ggggcgtaaa gtaataaata gtagccataa ataaaaagtg atacaccagc cactataacc
     5041 atccactttt ctcgatcatt cagcgtgttt aaataggttt tcacgttaac tccagagttg
     5101 caactacttg ttgatctttc gttgaggctt gagtttgttt cactttgagt tgtgattgtt
     5161 ttagtttatt ttcaattttt tgtaagcttt caaaatcaga acttaccaat gtgattgata
     5221 ttgttttgtt ttgatacctt aattgctcta ctgtgatacc actatctttt atggctttgg
     5281 ataattgatt taatataaac caaaatcgag tttgattttc tgcagtactc cccccaagaa
     5341 gctgacttat tcgaaactta ggactgatga cctgtttggc atcaggaaaa aactcgcgat
     5401 aaataaccgc ggtttgccgg tcaatttcat ctatttgtct attgagccaa tgtaaagaaa
     5461 gccaattaac aaaaagaata gaaattaacc agaagccgca taatccgcca accaataggt
     5521 atcctttttt aacccaatca gttgtttttc catgttgtat ttcaccttga caaagattga
     5581 gtggttttga ttttatcaat cttttactaa tccaggtgta tgagtgttct tcatttttgc
     5641 ttactggaga ggcgacaggt attttgctgt cttgaaacag atgagcggga ttgtgtggat
     5701 gatttttcag gtaagtttga gctaaatcac ctgagaggac acctttaaaa tcttcgttat
     5761 tgatcaatag ctcggattcg ctaatgatca attcttgaga tgctagcgca aaccagtcaa
     5821 gagttactgc atcaaactct atcccatgct catcaaaaac ttgaattaac tttttcattc
     5881 gttgtttatc aatcacaaca acgagatatt ggttattctg ataataaaat ttatcaaagg
     5941 caaaatgaag ttcttcgact ggttgagtta ctttatcctc tagtgcatag ggaattgcaa
     6001 ttctggcttt tctttcaggt agccatggaa ttgcaagact aagaagtatt gctctggtac
     6061 aggtttctac aataattgta ctggattctt tttgtaaatc ttttatttga gagaaattac
     6121 gaaattcagg gggagcaatg agctcaccca agtcagatat tttcaggcat acacaaccat
     6181 tatcatcgag atgtttgcta aaaataaaac acttatccat gagtcaaact ctgtattgtt
     6241 atggctgtca atttttcagt atataccagt aattattatt atttccagct ggcattaaac
     6301 ataagccctg tgccttttag tggcactagt ataccaatgt tgcttcaatt gctgggataa
     6361 taaataaacc gccagagcat attgagggct cgaattatag cgggtaatta caaaaaaatt
     6421 ggggtaggca atccagtatt ctttaccctt ccttgtaact aactcaatga ctcctgcacg
     6481 aggaggatga ttttgtgacg ctgtaattgg ttttactcca aagcttataa gttgtgagta
     6541 tcggtaattt gctgttttag ggttcatgcg aagttgtttg tatcgccatc cggagatttt
     6601 ggccggttga gcgatgcctt gatttaagtt ccaaccatgc ttatgcaaat aattggcgat
     6661 gctggcaata gagtcaccat tgtcattgac taaatctctt cgaccattgt tattaaagtc
     6721 cactgcataa tatcgataac tgcttggcat aaattgaggt tttcctatgg cacctgcgta
     6781 agatcctttg tattgagttg gggatacgtg ctgttctcgg cacaggagga aaaactcctt
     6841 taattcttta gtaaagaatg cagagcgttt ggggtagttg aaagccagtg tagccagagt
     6901 atccaagact ctgtattcac cctgacgctc accatacatt gtctctactc ctaaaatagc
     6961 tataatgatc tccggagaca ccccatacct tctctgaatt ttttccaatg ttttttgatt
     7021 ttcagcccaa aattgcaatc cgctttttaa tcttttctga gtaagaaaca gttctcggta
     7081 gacatcccag tttttctttt catagggctt ttccatagat tcgatgactt gaggttgaag
     7141 tttaacttgg ttcattactg tagttaactg attacgatta aagtgataat tcgttaccat
     7201 catgttaata aatgattgta cgtctttacg ttttgtaaat gttgtgtcgg aataggtagt
     7261 gaaagataaa aagaagagtg aaatgaaacc tagccagatg atttgtcgca tcatgtaccc
     7321 tgtatcgttg caaagcacga attttagtaa aaataaagcc tcatagcaaa tttttgacct
     7381 ttgtttatct taaatacagt gcatgaaatt ggtaaatgaa taagcaattt tcattctctt
     7441 aaaaatataa aaacgactga atttgctttg cctactttct gcatgcgaac atttaagata
     7501 gacttatgct atttgcaaga aggttagaac agttgaaaga tttaaaccga attcgaaatt
     7561 tttcaataat tgcccatatt gatcatggca agtctacgtt ggctgatcga tttatccaaa
     7621 tttgtggcgg gttgactgag cgcgaaatga gctcgcaagt tcttgattct atggatattg
     7681 aacgtgagcg aggtatcacc atcaaagcgc aatgtgtatc cttgaattac acagctaaag
     7741 atggaaagac ctatttattg aattttatcg atacgccagg gcatgttgat tttagttacg
     7801 aggtatctcg ttctcttgcc gcttgtgaag gtgctattct ggttgttgat gcagcacagg
     7861 gagtagaagc acaaacttta gcagtttgtt atacagcaat tgatcagtct cttaccgtat
     7921 tgccagtact gaataaaatt gatttaccgc aggcagaacc tgagcgggtt atttccgaga
     7981 tcgaagatat cattggtcta gatgctcaag atgcgattag agtgagtgct aaaagtggct
     8041 tgggtgttaa tgatgtttta gaggcattgg ttgcgaatat tccccctccg aaaggcgatg
     8101 ttcatgcccc attacaagca cttattattg actcttggtt tgatagctat ttaggagttg
     8161 tctcactggt tcgtattgtt aatggcgcca tacgaaaagg tgataaaatg cgagtgatgt
     8221 ccacaggcag ggcttatgaa gtggatcaag ttggtatttt tacccccaaa cggactaaac
     8281 tggatgcttt atatgctggt gaagtgggtt atgttgttgc tggtataaag gaaattcagg
     8341 gcgctccagt tggtgacact ttaactctgg atagaaatcc agctgataaa gttcttccag
     8401 ggtttcaacg cgttaagccc caagtttatg ctggtttatt tccggttagc tcggatgatt
     8461 ttgaggcatt tagagaggct ttggctaaat tgagtttaaa tgatgcttct ttattttatg
     8521 agccggaatc ttcagaggct ttgggatttg gttttcgttg cggattttta ggcatgctgc
     8581 atatggaaat cattcaggag agattggaaa gagaatataa ccttgatttg atttctacgg
     8641 cacctactgt tgtttatcag attgtaactc agaaggggga aactttatta attgataatc
     8701 cttcgcattt gcccccaacg ccgcaaataa aagagatgta tgagccaatt gttagagcaa
     8761 atattctagt gccacaagat tatttggggc ctatcattac cttatgtgtt gaacgacgtg
     8821 gtgttcaagt cagtatgacc tatagtggtc gtcatgtttc ggttgtttat gatattccta
     8881 tgagcgaagt agtctctgat ttttttgata gattaaaatc agttagtcga ggttatgcct
     8941 ctttggatta taacttccaa agattccaga ttgctgattt ggtcaagatg gatatattaa
     9001 taaatagcga acgtgttgat gctcttgctg tgattgttca tagagattcc gctcatagcc
     9061 gtggaaaatt aattgcggag aagatgcaac agcttattcc aagacagatg tttgatgtgg
     9121 ctattcaggc tgctatagga agccatatta ttgccaggca aacagtgaag gcattgcgca
     9181 aaaacgttac agcaaagtgt tatggtggtg atgtgacgcg taagcggaag ttgctggaaa
     9241 aacaaaaagc tggcaagaaa agaatgaaac aggtggggca tgtagaaata cctcaagaag
     9301 catttatggc cgtgtttcaa acagataaaa aaaagtagag ccgctcattt actaataagg
     9361 atgtgctgat cttttcgtca gcatctagac ataagtcaaa tcatgattaa attgtctttt
     9421 gtttaaaatt gaggatattg aatttatgaa ttttgcttta atattggtca tattatctgc
     9481 tgtaacagga ttgattgctt tattggattg gttgatttgg tctaaaaagc gtttgcctga
     9541 acaaaagtca ggtcgaatca ttgagtattc acgttctttt tttcctgtat tttttattgt
     9601 tctgctattg cgctcttttt tagttgaacc ttttcgtatt ccatcgggct cactcgagcc
     9661 tactttgttg gttggtgatt ttgtagcggt taataaattt tcttatggac tgagacttcc
     9721 agtatgggag aaaaaaatag tgccaattgc taatcccaaa acgggagaaa tcgcagtatt
     9781 tcgctggcca cctgaaccat cttatgacta tattaaaaga gttattggag tgcctgggga
     9841 taaaatttca tatcatgata aaaaactaac tattaatggc aaagaggtga aacagacctt
     9901 tgttgagtat actaccgatg aaagttctgg caaggctgtg acaaagtata aagaagattt
     9961 aaacggtgtg gtgcacgaca tatttgtcag acctgatgcg ccagccgtag attttgatat
    10021 agttgttcct gaggggaatt actttatgat gggagataat cgggatgata gcgccgatag
    10081 tcggttctgg gggtttgtac ctgatagcta tttaagagga aaagcatttc tggtctggat
    10141 gagttggaat ggcaaaacag ataatgtacg ctggtcaagg ataggcagat taattcattg
    10201 agggaataga gcatgagaaa acaacaggga atgacattga ttggcatgct gtttactgct
    10261 gttgtggtga taatggctgg tatacttgtt atgcgggcag cgccagttta tattcaacat
    10321 tattcaattt tgcattcaat caaagcatta aattcaacac ctgcttcttc tttgtctggg
    10381 gatcccttga ccgacgttac aactttaagg gaaagtgtga ctaaacgatt agatatcaat
    10441 ggggtagaag acttaaaacc aggagagctg gttattactc cttcaggccc caataaattt
    10501 aaaattaagt taaaatatca agtcatcaaa cctctaattt ataatatgag cttattattt
    10561 aattttgaag ataattttga ggtaataaca ggtagtgaaa attaatttag aaagattgtg
    10621 cagacgttta aactatcatt ttaataacat ggcctatttg aagcaggctt tgacgcattg
    10681 cagtgctggt agcgataatt atgaacgctt tgagtttctt ggagactcga tattaagttt
    10741 tgttattgcc aatgagctat tcaatcgttt tccacttcac agtgaagggc aattaagtcg
    10801 attgcgctct tttcttgtta aaggtgaaat gttagcagaa atagccagag aaataggttt
    10861 gggtgattat ctatttcttg ggcaaggtga gttaaggagt ggtggatttc gccgcacatc
    10921 tatattagca gatgcccttg aggctatatt ggcagccatt tatttggatg gaggaatgac
    10981 tgctgcaaaa cagattattt taatgcttta tagttcacga ttggatgatc cggatttaaa
    11041 tcattgttta aaggatgcaa agacacaatt acaggagttt ttgcaagcat caaaattcgc
    11101 tttacctgaa tacgtcctaa ccaaggttga gggggatgag catgctcaaa tcttccatgt
    11161 aacatgcaca attgagggag taagtcaggt agcttatggc acagggccta atagaagaaa
    11221 agctgagcag ttggcagcaa aagccatgtt ggaacagtta caaggttaaa tggtattgat
    11281 cagcagaacc tatttcttac tccaggtttt ccagggcatg acatatttct aaccactctt
    11341 cttctgtctt cattaaagaa gaatgagcga catctcgttc catcaaaaga ttatccaatt
    11401 tgtttttctg ctttgtgtct tcatacagag aaacgtcagc tagctgctta tcaagaaggc
    11461 gcaattgatt ctgatagtgc tcaatgagtt gctccagctt ttttaatttg ttttgatagc
    11521 tttttctttc tttatattca tttggagagg tagctaattt ggattccttt tctgaatttc
    11581 tggtttgcag ccatagatag taatcatcaa gatccccttc aaatggttgt acttttcctt
    11641 gataaataag ataaaaatta tccacacttg attttaacaa atggcggtca tgcgaaatca
    11701 aaattaaggc accctcataa gtttgcagcg ctaattcaat tgctgagcgc attccaagat
    11761 ccaaatggtt tgttggctca tccagcaaaa gaaggttggg ttttagccaa accagtcttg
    11821 ctaatgccag gcgggctttt tcaccacctg aaaagtgctg aatggattgt accgccatgt
    11881 cccctctgaa attaaaccca cctaaaaaat cccgtatggt ttgttctctt acctctggcg
    11941 agagagtttg cagtgtttct accggactta atttacagtc taattgctca agttgatgct
    12001 gagcgtagta gcctatttgt aagtgtggag aacgatgaat atctccactt aatacggaca
    12061 aatctgctgt aagcgtttta attaaggttg attttccttc accgttaggt cccagtagcg
    12121 caattctatc gccgggatta agagagagtt tgattttttt caaaatcgga ggatgattaa
    12181 tatatcctgc atcaacttga ttgcattgaa ttaaaggatt tcctgcccga ggacaaggga
    12241 aaaaatcaaa agaaaaaggg gagtcagctt gggctgctgc gattatttcc atcttttcta
    12301 tagctttcat tcgactttgc gcttgtttgg ccttggttgc tttcgcttta aaacgagtta
    12361 caaaagtcat catatgtttg attttggttt gttgcttttc atgcattgct tgttgtagag
    12421 ccagttgttg cgcatgaatt ttttcaaagc tactatagtt tcctgaataa agtgtcagat
    12481 tacgattttc tatatgtaat atatgagtaa caaaggcatc aagaaattct ctatcgtgtg
    12541 aaataagaat aatagtactt gggctttgtt ttaaatagcg ttccagccag aagatggcct
    12601 ccatgtctaa atggtttgta ggttcatcaa gcaagagtaa atcggcgggt ttcattaagc
    12661 accgagcaag atttaagcgc attctccagc caccagaaaa gctgttaacg ggctcttgct
    12721 gttgatttcc actaaatcct aagccagaca taataactgc tgcttgcgcg ggcttactgt
    12781 aaccaccagt atgagagagt tcctcgtgac attgcaaaac ttctgcatca tcgccgtttt
    12841 tttcagcttc tgaaaggcgc tttagcaatt ttatgtattg ttgatctccg gcaagaacaa
    12901 aatccaaagc tttttcattg ctatcaggta tgtgttgaga caaatgactg atactgagtt
    12961 gtggattaat tagatattct ccagtatcag gagataattg acctaaaaga aaatcaaata
    13021 atgttgattt tccacatcca ttatgaccga taaggccaat tttttgcttt tcataaagac
    13081 tgacattgac cttatcgagt aaaattttat ctcctctgga taaagtaatt tgacgcaatg
    13141 taagcatgtt ataccgtaag cttgaactaa ttagtcattt tatcataaat tagcttaagc
    13201 ttcctattga tgatgtaagc gagttgaata tttgcataag ttcaaaaccg gattttcttt
    13261 aaaaataaac tagggtctgt tgacatttct actatcttgg ccgaaaagcc ttgattttct
    13321 gtgctccgtt actcacatac ttctatgtat gctccgctac ggtgctcgaa aatcaaggct
    13381 tttcgactca agcgagcgaa atgtcaacag accctattcg ctgggctgaa ttattttctt
    13441 tcgcgctgat tgataacgtg cttgcttttc tatatcaata tacctgtcgg ttgtagcact
    13501 tgaactgtgc ccggcatcat ctcgcacatg ctctctgggc ctgattttta catcttctga
    13561 tataccagta tgccgcagcc aatgcaccgt ggcagcagat agattatccg cttcctctga
    13621 taaaccatta agtcgcagtt gttcttctgc caagtcaaaa caatgctgaa caattcgcct
    13681 tataggagct gtgttactca ttggaccatg accacgcagc ttaggtagta aaggtgaatt
    13741 atcagcaggt gatggaagag gggtaagtcc aagaaataat cgccaacggg taagactttc
    13801 cagcatagcg tcgcttaccg caatttgtcg ttccttattt cctttcccca cggtggtaaa
    13861 ccaccagtgg ccattactgt ccttggcaaa atcattcata ctgggtaccc atctctcact
    13921 ggcagcaagt tctgatatac gaagatacat gccaaataac agacttaaaa taaaacgagt
    13981 gcgttcatgt ttttcagggg aatcttcagc caaactttca gctgctctca ggacagcctc
    14041 ccattgaacc agacttaatc gtctaacagg cgcgttatgc tgccttttgc gaataaattt
    14101 gcttttttgc ctaataaggg caactggatt agatatcaga tattcttcag caattagaaa
    14161 gttgaacaga gtacttaaga tggcgaatat ttcttgcaca gccccctgag ataacttgaa
    14221 ttgatctatt gatgcaagat gcccattttt cctggaaacc ttacttagtg tcaccacaaa
    14281 aggtcgccac tcttcattag gtgtccgttt accatttttt tctataaatc gtggaacttt
    14341 ttttagacta atccatgttt taggagggtt ttgacagaaa tggatgtatt gttcaatatc
    14401 atcacgcctt aaagaattaa tagtagagtt tttgacaagc catgaccact gtaacaaacg
    14461 ttctgtttct cttctgtaac tgttgaaagt accttcgctc cctatgtagc tttttaaaaa
    14521 gcttaggcaa taagtgaaat cattgtgaaa tcgagtatca ggcaatatta cattttcatg
    14581 gtttttatgg atgtggttga gattatcaaa caagggtaat agttttgttt tcatgatcat
    14641 cacttatctg ctgataattc attatcctaa cctaattgct gatttaagtc atgagggtga
    14701 aagtgcatag ttatagagta cgcgagttat tgttgataat agtgctgagg caactacgta
    14761 agcacttgta aagggcaata aattgacgaa aagtactgaa gaaattcaga ctatatcaat
    14821 aaataaaaat tatatgctaa aggatattga ccttcgtgat agcacctagt attatctgcg
    14881 ctctgtcctt gggacgggta tcggggtgta gcgcagtctg gtagcgcgcc tgctttggga
    14941 gcaggatgtc gggggttcaa atccctcctc cccgaccatt taagcgcccg tagctcatct
    15001 ggatagagca tcggccttct aagccgaggg tagcaggttc gaatcctgcc gggcgcgcca
    15061 gccgccttgc ttttgcagta ttatagctgg aacagggctt acgaaaccat ctatagttac
    15121 gtaattcttt aatgaaaatc tattttgtgt aaataactat atagaaaatt atgaatttgt
    15181 aatttgggaa gtttcgtaag ccctgtttaa gagtgttttt ggtgagcgta gctcagttgg
    15241 tagagccccg ggttgtgatc ccggttgtcg tgggttcgag tcccatcgtt caccccattt
    15301 gcaacaaata tttttttgag tctaccgcta aagattccat ctgagtcttt acaagactga
    15361 attactgatt gataatccaa cctgattgcg aaagtggcgg aattggtaga cgcactggat
    15421 ttaggttcca gcggggcgac ccgtgagagt tcgagtctct cctttcgcac catttcatga
    15481 taacttccga gaggtgaatt aatgcaagtt tctgttgaga ccctagaagg tttggagcgt
    15541 aaggttacag tttctgtacc tacagagaaa gttgaggaag aggtgagctc gcgtctcaga
    15601 aatcttgctc gcaaagtgaa aatagatggt tttcgtccag gcaaagtccc ttttaatgtt
    15661 gtaaaaagcc gtttctctga tagtgtgcgt gaagaagttg cccgagaaat ggtccaatct
    15721 accttatatg aagccttaca aaaaaatgaa ttggttccag caggttatcc tcatgttgag
    15781 cctttagaaa tagaaccagg taaggatttt aaatacactg ctgtatttga agttatgcct
    15841 gtttttgaaa ttgtcgagct caatcaagct ccagtggaat taattcgatc tgaagtcact
    15901 gataaagatg ttgacaacat gattgaaaaa ttacgagagc aaaacaaaga gtggcatgaa
    15961 gtaactcatg cggtcaaaaa aggtgataaa gttgttattg attttcaggg atttttggat
    16021 gataagccat tccagggcgg cagtgctgaa ggatatgagc ttgtaattgg ttctggttcg
    16081 atgattcccg gatttgaaga tgggattatt ggtgggaaaa ttgacaaacc attcgatatc
    16141 aaagtgagtt tcccggaaga ttatggccat aaagatttgg ctgggaagga agcaacgttc
    16201 aagatcacta tcaaaaagat tatggaaggc aagttgcctg ctttggatga ggcatttgcg
    16261 gagaaattta atataaaaga aggtggtata gagtctctta aaaaggatat tagggaaaat
    16321 atggcgcgtg aattggaacg tcgcgttaac atgatgaaca gagagaaact gtttgattca
    16381 ttaatgtctg tgaatcatgt tgagttgcca atagctttga tagataaaga aattgagcat
    16441 ttaaagcatg atatgtatca ccgattgttt ggtcacgaac ataaagacga tgaaaaaatt
    16501 cctgatttcc caagggagct atttgaagaa caagccaagc gacgtgtaca cttgggctta
    16561 ctttttgccg agtatgtaaa aaaacacgaa attgttgcag acaacgacaa agttaatgct
    16621 atgattgata aatttgccag tgcttacgaa agtcctgatg agcttcgcgc gtggtatcaa
    16681 agcagcaaag aacatatggc tgaagttgaa gcattagtta tggaagatat ggttgctgat
    16741 aaaatagcag aagatgcgaa acttaaatac aaaaatatgg attatgattc agtgatgaat
    16801 cccaaaaagg gcactgagaa aaaaggagag taagcatgcc aggctattca gataatatta
    16861 tccgtaatgc aagcggatta ataccaatgg taattgagca aacctcgcga ggtgagcgtt
    16921 cctatgatat ttactcaaga ttattaaaag aaagaattat ttttctcttg ggtgaagtag
    16981 aagatcatat ggctaacctg gttgtggctc agttgttgtt tctggagtct gaaaatccag
    17041 agaaagatat atctctatat attaattctc ccggcggagt cgttactgca ggtcttgcta
    17101 tctacgacac catgcaattt attaagccag atgtaagtac attatgcata ggccaggcag
    17161 caagtgcagc tgccttgcta ctttgtgccg gtgctgatgg caaacgattc tgcttaccta
    17221 actcaagagt tatgatacat cagcccttgg gtggctatag aggtcaagca acagacatag
    17281 aaattcatgc tcgagagacc ttggcagtta gggaacgttt gaataatatt atggctaaac
    17341 acactaaaaa aacacctgat caaatcatgc gtgatacaga aagagataac tttatgagcg
    17401 ctactcaggc tatggagtat ggtctaattg ataaagttct ctatgacaga caaattgctg
    17461 gacattctac agacttataa ctttgctaga tactcctagt ttatctgagt ataatataag
    17521 ttggtttgat agtaatgtgg tggcgcaata ttatctgatt gtctaaaatt ttacatgtaa
    17581 ttactattaa ttagtaatag ctgtaagaag gggtttggtt atgagtaaat ccggtaacgg
    17641 aaatggagat aaggtcttat attgctcttt ttgtggcaag agtcaacatg aggtaaaaaa
    17701 attaattgct ggaccttctg tgtttgtttg tgatgaatgt gtagagctat gtaatgatat
    17761 tattcgagaa gaaacacatg aaactcatga agaaactgag acacgtctac cgacaccaaa
    17821 agaaatatct aactttctcg atgaatacgt aatagggcaa caacatgcca aaaaggtttt
    17881 atcagtagca gtatataatc actataagag attacagcat aaaagcgagg atggtgttga
    17941 actcggtaaa agtaatatac tacttatagg acctactggc agtggtaaga ctcttttagc
    18001 tcaaactctg gcgcggattc ttaatgttcc ttttgccatg gctgatgcca cgacccttac
    18061 tgaagctgga tatgttggtg aagacgttga aaatatcatc cagaagttat tacagaagtg
    18121 cgactatgat gtagataagg cacagcaagg tattgtctat atcgacgaaa tcgataagat
    18181 ttcacgcaaa tcagataatc catctatcac cagagatgtt tcaggagaag gtgttcaaca
    18241 agctttactg aaattaattg aaggtactgt tgcatctgtt ccaccgcaag gtgggcgaaa
    18301 gcatccacag caagaatttt tgcaagtaga tacatcaaat attttattta tctgtggtgg
    18361 cgcttttgca ggtttggata aagtaatacg tgagcggagc gataaatcga gcataggttt
    18421 ttctgctcaa ttaaaaagca aaaaaagcag taatgatgaa gtttcaaaag ttttagggca
    18481 attggaatca gacgatttaa ttaaatatgg gttaattccc gagtttgttg gacgtttgcc
    18541 tgttgtaacc actttacagg aacttgatga agcggcattg attgatatat taacgagacc
    18601 gaagaatgct ttaacaaagc aatttcaatc acttttcaaa atggaaggtt ctgaactcga
    18661 gtttagagac gaagctttga ttgctattgc taaaaaggcc ctcgaaagaa aaatgggcgc
    18721 aagaggtttg cgatcaatac ttgaaaatat tcttttagac accatgtatg acttaccttc
    18781 cttggaaggc gtgaataagg ttgttattga tgagagtgta gtgaatggac tatctaaacc
    18841 gattctcatt tatgaacagg atgagaaaaa aagtgcgtct ggtagtaaag attaagtaag
    18901 atgttgattt ctgcaataat ttaaattgtt gcagaagtca ttttatcttt cattcacaaa
    18961 atatatttaa agctgatttt gtagcttaaa ataacaaatt tagtcatctt aatagcaata
    19021 gactaactct cacattacga attgttca
//
LOCUS       DBNCWK010000052        18657 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.052, whole genome shotgun sequence.
ACCESSION   DBNCWK010000052 DBNCWK010000000
VERSION     DBNCWK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18657
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.052"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(58..1542)
                     /gene="pgi"
                     /locus_tag="AC06IO_001578"
     CDS             complement(58..1542)
                     /gene="pgi"
                     /locus_tag="AC06IO_001578"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIO9874765.1"
                     /translation="MKSHTELLSWNLLQKEADRVRLNSDSLTCVVPDSNNYESSKQIN
                     CIEYDYSRQRVNRTIIDLLIDLANEVKLQEKIDNLINGKKINISENRPALHTALRDLG
                     NKSIMIDGLDIMSAVINTREKIKVISNQIREKKWLGHSGLPITDIVNIGIGGSDLGPR
                     VCINALSNYISKEFNYHFISDVDPASFNDVIAKINPQTTLFIVSSKSFTTKETLLNAR
                     KAFALYEDTASIDQHFIAVTAHPERAYQMGIKTVLPIWDWVGGRFSFCSAVNLITAIA
                     IGYEQFVELLAGAHDIDTHVQFTDFKNNIPVLMALIGIWNNNFLNIHNLLILTYSKKL
                     EYFVPYVQQLDMESNGKSIDVNGRMVDYATGPIVWGGLGNQAQHSYFQLLCQGTHRCV
                     GDFITLKTNDEHEINSMCHYKMKVLSEGIQTIENPYGYIPGNMPMNHLILSDCSPYTL
                     GALVALYEHKIFVQSVIWNINPFDQPGIESAKSAHREITLSSES"
     gene            complement(1596..2675)
                     /gene="rfbB"
                     /locus_tag="AC06IO_001579"
     CDS             complement(1596..2675)
                     /gene="rfbB"
                     /locus_tag="AC06IO_001579"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIO9874766.1"
                     /translation="MICQMLSTILQKSKNLTMKNQMNNLLVTGAAGFIGSNFVKFMND
                     KYPEIKIISLDKLTYAGNKANLSEMAECKNHLFVQGDILDKSLVLSLLREYEIDTLVH
                     FAAESHVDNSIDNPQIFLETNVIGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVY
                     GSLEREEPAFTEKNSYQPNSPYSASKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQH
                     KEKLIPKVVFACVNQLPITVYGNGSNIRDWLYVMDHCEAIDTIIQKGALGEVYNIGGN
                     NELDNLSLIKMICQMMDDLKPMEKPYHSLITFVEDRKGHDKRYAIDNSKIQKELGWVP
                     QGDFVHKLSNTVHHYLTRYEREINI"
     gene            complement(2635..3519)
                     /gene="rfbD"
                     /locus_tag="AC06IO_001580"
     CDS             complement(2635..3519)
                     /gene="rfbD"
                     /locus_tag="AC06IO_001580"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIO9874767.1"
                     /translation="MKILVTGANGQVGTEIIKRFSSSEHEVYACTRDILDCSKLERVH
                     DVLSEIKPDLIINAAAYTAVDKAEDEPDLAHIVNAEFVCRLVNYCTLKNVPLIHLSTD
                     YVFDGEKEGAYHETDIPHPVSTYGRTKWEGEQAILSQLKKYIILRVSWVFGEQGKNFV
                     KTILNLASSRKELNIVSDQSGRPTSARDIARVLFEIVQKIRHSSFDYWGLYHYAGQGV
                     TNWHEFANVFLNMAKEKQLLLTLTDLNPIKSQEYPTRATRPKNSVLNTTKIETILGIK
                     SCSWKNDLPDVIDHFTKK"
     gene            complement(3520..4083)
                     /gene="rfbC"
                     /locus_tag="AC06IO_001581"
     CDS             complement(3520..4083)
                     /gene="rfbC"
                     /locus_tag="AC06IO_001581"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIO9874768.1"
                     /translation="MNIINTQIDELKIIEPKIYGDERGFFYESFQAKRYEELLGITDR
                     FVQDNFSRSQKGVLRGLHYQSQQTQGKLVSVLAGEVFDVAVDIRLGSPTFGQWVGVIL
                     SGENKRQFWIPKGFAHGFYVLSAMADFAYKCTDYYHPESEFSIHYLDPQLAIDWPLGE
                     QVQLSPKDAAAKLLNLIDAELLPRYQA"
     gene            complement(4173..5675)
                     /locus_tag="AC06IO_001582"
     CDS             complement(4173..5675)
                     /locus_tag="AC06IO_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIO9874769.1"
                     /translation="MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVT
                     DGDIRRLLLKGFTLDSTLEELSEHSSKALPVSATIQDAYHLMQEYELDHIPVIDESNR
                     PIRLIHRRELSSNILLSSPHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINI
                     KSAVALSSGTAAIHLALVLLDVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDT
                     WNMSPVALERALRESKAKNQMPKAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAES
                     LGATYNNKYSGTFGKLGVFSFNGNKIITTSGGGMLVSEDESLIQRARFLSTQAREPMP
                     HYEHTVVGYNYRMSNVLAGIGRGQLKVLEKRVKARRAIFERYKQALESYPFIDMMPEI
                     TQGYSTRWLSTLVIKPTPNKIKPEEVIEQLKPFNIEARRTWKPMHRQPLFAGSKYYPH
                     DEQFSVSDYLFDHGICLPSGSNLTPRDIDRVIHCLNDIFIKYKQNNFEVA"
     gene            complement(5686..6294)
                     /locus_tag="AC06IO_001583"
     CDS             complement(5686..6294)
                     /locus_tag="AC06IO_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIO9874770.1"
                     /translation="MHTNLKIIGCGGHCKVVIDALSLTQHSLQVSLCDSNKSLLGQEV
                     CGVLVDSTMDSLADYIGYVHVSIGNNQARKNVIDLMNSNANLFTIIHPAAIIAKSARL
                     GAGSFIAAQAILGPECQVGEGCIINHSAVVDHEVIVGSCSHIAPNSTLGGRVKIGERV
                     LIGAGAVVLPGVTIDDGAIIGAGSVVVKDVKENAVVKGVPAV"
     gene            complement(6284..7417)
                     /gene="neuC"
                     /locus_tag="AC06IO_001584"
     CDS             complement(6284..7417)
                     /gene="neuC"
                     /locus_tag="AC06IO_001584"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HIO9874771.1"
                     /translation="MTRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDA
                     LYGNTVNEIKADQFSICGIIPVDLDNAQHSSMAKAIGHELLGFTEVFESETPDVVLLL
                     GDRGEMLAAAIAAIHLNIPVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRL
                     IRMGEKEETIFQVGAPGLDEIMQYKTSTRDVFNQRYGFHPDKKICLLIYHPVVQEVDS
                     IKIQFQSVIQAALATNLQIICLEPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFI
                     DCLANSDVMLGNSSSGIIEAASFNLNVVNVGSRQNLRERSDNVIDVDVTYDAILTGLR
                     EALNKPKIKYSNCYGDGKTSERCYQLLKTIPLHSQILNKCNAY"
     gene            complement(7417..8439)
                     /gene="legI"
                     /locus_tag="AC06IO_001585"
     CDS             complement(7417..8439)
                     /gene="legI"
                     /locus_tag="AC06IO_001585"
                     /EC_number="2.5.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetyllegionaminate synthase"
                     /protein_id="HIO9874772.1"
                     /translation="MTCFIIAEAGVNHNGDLQLAKELVYAAKESGADAVKFQTFKADT
                     LVNKTVEKAEYQKNNAPESSTQYEMLKALELSEEDHYLLSELANSLGIEFMSTGFDEQ
                     SIDFLISLGVKRLKIPSGEITNVPYLQHCASKKLPLIISTGMCDLQEVRVAIDTVKPY
                     YGNSLSDYLVLLHCTSNYPAAYQDVNLKAMQTLADEFQLPVGYSDHTLGILVPTLAVG
                     MGACVIEKHFTMDKSLPGPDHLASMNPEEMKNLVQSIRDAETVLGSGEKKPSDNELPI
                     RALVRRSITLRRDLVKGAQISKEDLILLRPGTGIAPSEISNIVGARLSMNLSAGTTLL
                     WEHIEA"
     gene            complement(8436..9134)
                     /gene="neuA"
                     /locus_tag="AC06IO_001586"
     CDS             complement(8436..9134)
                     /gene="neuA"
                     /locus_tag="AC06IO_001586"
                     /EC_number="2.7.7.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N,N'-diacetyllegionaminic acid synthase"
                     /protein_id="HIO9874773.1"
                     /translation="MRILAVIPARAGSKRLPGKNTRLLAGKPLIAHTIVAALQSSCCE
                     EIVVSTDSKQIADVAVQYGASVPWLRSEDLATDTSDVIHTVIDLLFKFQQMEVFFDSV
                     LLLQPTSPFRKPETIRHAVEIHQATGKSVVSVSPISLKPSWCRSIDSQGNLVKPELFQ
                     DLEIYCNENPIYKLNGSIYIATAKQIIENKSFYSEPTKPLLLNSISESIDIDTPIDWA
                     LTEKLMELNQEALV"
     gene            complement(9147..9788)
                     /gene="hisH"
                     /locus_tag="AC06IO_001587"
     CDS             complement(9147..9788)
                     /gene="hisH"
                     /locus_tag="AC06IO_001587"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIO9874774.1"
                     /translation="MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLV
                     LPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGICLGMQMMLSRSTEFGQHEGLDL
                     IAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
                     AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLNIIQQFLQI"
     gene            complement(9785..10546)
                     /gene="wbuZ"
                     /locus_tag="AC06IO_001588"
     CDS             complement(9785..10546)
                     /gene="wbuZ"
                     /locus_tag="AC06IO_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl amidation-associated protein WbuZ"
                     /protein_id="HIO9874775.1"
                     /translation="MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGA
                     DELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAI
                     NTAATKNPTLISDVARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARN
                     GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCES
                     KADAVAMADVLHYKRIGLPEIRKYALDNGIHVRVL"
     gene            complement(10546..11901)
                     /locus_tag="AC06IO_001589"
     CDS             complement(10546..11901)
                     /locus_tag="AC06IO_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HIO9874776.1"
                     /translation="MSKIYAPKSIDLSSFQLPDEKLQTKYGLPHKVEFCKSCVISNQR
                     PNSAVEYEHKKESKKHTIHFDDEGICDACRVAERKKSTINWEERDRQLRELCDRFRSK
                     DGSYDCVVPGSGGKDSFYAAHILKYKYGMNPLTVTWAPHMYTPWGWRNFQSWIHAGFD
                     NHLFTPNGRVHRLLTRLAVENLFHPFQPFMIGQKAYAPKMALLHKIKLVVYGENEAEY
                     GNPIGDTESAKRDWKYFTADDKSKIFLGGTSVQELKSDFGLNDNDLDAYLPADPQQIE
                     EQQVEVHYLGYYLKWHPQSCYYYSVEHGGFEASPERTPGTYSKYNSIDDKIDDFHYYT
                     TLTKFGIGRATYDASQEIRSGDITREEGVALVKRFDQEFPERFAEEIFKYLSINPKEF
                     PIASQMFEQPIMDRAYFMALADTFRSPHLWKKEGEQWKLRHQVTNLEKTKAEYLDLET
                     V"
     gene            12088..12906
                     /locus_tag="AC06IO_001590"
     CDS             12088..12906
                     /locus_tag="AC06IO_001590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9874777.1"
                     /translation="MSLKAMYNQIAENYATANRFGSISESHHVAIEQMRKFHLGLKPH
                     YKILDLGVGNGSFLQKLHHLMPMANFTGIDVSSEMLKRASEALPLTTIEGSAAEANKF
                     LPAHSQDLVLAHFINAYIPINVLFDEAKYLTRANGHFSLITTTYDSFPVAQQQLANFI
                     AQDTILSRVVGHYYKSIVKNTTVAANLDELLLAFKQHQFNILDHQRIEIPITLNNIDE
                     LALFGIEGTWFLNTLSIRMLPKYFLIQRLKRLFSKIFTFPYHDTHIIDVVLARK"
     gene            12945..14453
                     /locus_tag="AC06IO_001591"
     CDS             12945..14453
                     /locus_tag="AC06IO_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIO9874778.1"
                     /translation="MKLISQAVKNYLPELSLRQKQTNNIIFITFWSQFSVYALNTILV
                     LFLTRPLIAQGLGYSQAKAYAFIGVTQATGYLMPILGGYMADNIIGVRRSILLGSIML
                     ACAYLLVMLSGYTISSLGDQLFIIAFAFIPATNSLLMGTASSMVSHIYSDDAIKAKSA
                     MTYYYMAINVGALLATMIAPVLLESRYGPLSVLTLTFIGKSIAALNFAKRYSIYDSVI
                     WGMDKSKFSNQGLLRLVAYIAAIYSLTLYAYTHVYIASTLIGAGCAIGILWFLIKTIM
                     LKGETRSKQMIAVLLIIEAIVFFIIYNQMNSTLVLFAENNSDLSFLWFKISPAQYQML
                     NPLLILLIGSQLPRFYRFFSRFTIPYQFAAGTILSGIALLVMAFAAQNAINGLVNGNY
                     IALTYILISIAELWVSAIGLSMIGLYCDARAIAFAMGVWYLASSMSNAISGRIAGWVA
                     IPENINSALESLPYYKNYYLIMGISALGLGILMYFVAYYLHKIMKRRGIELA"
     gene            14854..15135
                     /locus_tag="AC06IO_001592"
     CDS             14854..15135
                     /locus_tag="AC06IO_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIO9874779.1"
                     /translation="MFWVYILQCSDKSYYTGQTDNLEKRLTQHQDKMIPGCYTSTRLP
                     IQLKFSQEFMSREEALSAERQIKGWSRRKKEALINGDWQALSDYSKRKN"
     gene            complement(15447..16436)
                     /gene="lipA"
                     /locus_tag="AC06IO_001593"
     CDS             complement(15447..16436)
                     /gene="lipA"
                     /locus_tag="AC06IO_001593"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIO9874780.1"
                     /translation="MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPK
                     WLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRAC
                     RFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
                     IKKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQ
                     TLNVLAFAKKYRPDVLTKTSLMLGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKN
                     HLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVFKRDNLGLDV"
     gene            complement(16588..18138)
                     /locus_tag="AC06IO_001594"
     CDS             complement(16588..18138)
                     /locus_tag="AC06IO_001594"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIO9874781.1"
                     /translation="MIDQELKKKIVNTISQYEHKCDKMQNQINTLRNVINQLIINPIG
                     INYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGV
                     ESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKELEELPQIT
                     KVDNPSKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTII
                     DNLTELVVESFNVEQNRFKGFLQQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQ
                     DNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIREEEYAKQVSELQAR
                     LQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYEEHILDSFQRWKRSSSALSLAV
                     CDIDHFKRINDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKE
                     AFGILEKLRKLVEDCLFYYREKKVDVTVSFGLTTVTEKDDIESLFMRADNAMYKAKNA
                     GRNRVEIL"
     gene            complement(18158..18430)
                     /locus_tag="AC06IO_001595"
     CDS             complement(18158..18430)
                     /locus_tag="AC06IO_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIO9874782.1"
                     /translation="MDDNNSEFKHLFPIAVEVTEGKTYVWCGCGKSKTQPFCDKENCG
                     DKAVSFIADLTEDIYFCNCKQTKSPPFCDGSHAKILLEVVKKRQNK"
BASE COUNT         5576 a         3928 c         3138 g         6015 t
ORIGIN      
        1 aataaaatat aaagtttagt ttaacaatta aactaattta tagcattgag cgccccttta
       61 tgattcgctt gacaatgtga tttctctgtg agcactcttg gcgctttcaa taccgggctg
      121 atcgaatggg ttaatattcc atatcacact ttgtacaaaa atcttatgtt catagagtgc
      181 caccagagcc cctaatgtat aaggagaaca atctgacaaa atgagatgat tcattggcat
      241 attgccagga atatacccat aaggattttc aattgtttga attccctcac ttaaaacttt
      301 cattttataa tgacacatgg aattaatctc atgctcatca ttagttttca aagtaataaa
      361 atcgccaaca catcgatgcg taccctgaca taataattga aaatagctat gctgtgcctg
      421 atttcctaat ccaccccaaa cgataggtcc agtggcgtaa tctaccattc ttccattcac
      481 atcaatggat ttcccattac tctccatatc cagctgttgt acataaggaa caaagtactc
      541 cagttttttt gaataggtaa gaataagtaa attatgaata ttcaaaaaat tgttattcca
      601 tataccgatt aaagccatta gcacaggaat attattttta aaatcagtaa attggacatg
      661 agtatcgata tcatgggcac cagccagcaa ttcaacaaat tgttcataac caattgcaat
      721 cgcagtaatt aaattaactg cggaacaaaa agaaaatcga ccaccaaccc aatcccaaat
      781 cggtaaaaca gttttaatac ccatttgata agctctttcc ggatgtgcgg ttacagctat
      841 aaaatgttga tcgatagagg ccgtatcctc atacaatgca aaagcttttc tggcatttaa
      901 tagagtttct tttgtagtaa atgatttgga agaaacaata aataaggtgg tttgcggatt
      961 aatttttgca ataacatcgt taaaactggc cggatcaaca tctgagataa aatgataatt
     1021 aaattctttg gaaatataat tagacagggc attgatgcat acacgaggac ctaaatccga
     1081 gccgccaata cctatgttta caatatcagt tattggtaga cctgaatggc ccagccactt
     1141 cttttctctg atctgattgg atataacttt aattttttca cgagtattta taactgcgga
     1201 cataatatcc agtccatcaa tcattatcga tttattacct aagtccctaa gtgcagtatg
     1261 tagtgcaggc ctgttttcgc tgatattgat ttttttacca ttgattaagt tatcaatttt
     1321 ttcttgtaat ttgacttcat tagccaaatc gataagcaaa tcaatgatgg ttctgtttac
     1381 tctctgcctg gaatagtcgt actcaataca attgatctgt ttactggatt cataattgtt
     1441 ggaatccgga acaacgcatg ttagagaatc tgaatttaga cgcactctat ccgcttcttt
     1501 ttgaagcaaa ttccatgaca ataattctgt gtgagacttc atgctatttc taatcatcaa
     1561 aataaagtct atgttacctg attattttca atttattaaa tattaatttc gcgctcatac
     1621 cgtgttaagt aatgatgaac tgtatttgat aatttatgaa caaaatcacc ttgtggaacc
     1681 caacccaatt ccttttgaat tttactattg tctatggcgt atcgtttgtc atgacctttt
     1741 ctatcttcaa caaaagtaat caatgaatga tatggttttt ccataggttt taaatcatcc
     1801 atcatctgac aaatcatttt tatcaaactt aaattatcca gttcgttatt accacctata
     1861 ttataaactt cccctaatgc tcctttttga ataatggtat caattgcttc gcaatgatcc
     1921 atgacataca accaatcgcg aatatttgat ccgttgccat aaacggtgat gggcagctga
     1981 ttgacacaag cgaatactac cttaggaatc aacttctctt tatgttgatt aggcccgtaa
     2041 ttattcgaac aattcgaagt ggtaacaggt aatccatagg tatgataata agctctgact
     2101 atatgatcag aacttgcctt ggacgctgaa taaggggaat tgggttgata tgaatttttt
     2161 tcagtgaacg ccggctcttc tctttctaat gaaccataca cttcatcagt ggatacatga
     2221 tgaaaccgac actttgattt atcccattgc ctttcattca accaataaat ccttgccgcc
     2281 tctaacagag taaaagttcc aattacattg gtttcaagaa atatttgcgg attatcaatg
     2341 gaattatcca catgggattc ggctgcaaaa tggacaagcg tatctatttc atattccctt
     2401 agtaaggata acaccagact tttgtccaat atgtctcctt gcacaaaaag gtgattttta
     2461 cattctgcca tttcactcaa attggcttta tttcctgcat aggttaattt atccaaactg
     2521 attattttga tctccgggta tttatcgttc ataaatttaa caaaattact tcctataaag
     2581 cccgcagctc cagttaccaa aaggttgttc atctgatttt tcatagtgag attcttactt
     2641 ttttgtaaaa tggtcgataa catctggcaa atcatttttc caactacagc tttttatacc
     2701 caatattgtt tctatctttg ttgtattgag taccgaattt ttaggccttg ttgccctagt
     2761 aggatattct tgtgacttaa ttggatttaa atcggttaaa gtcaagagaa gctgtttctc
     2821 ttttgccata ttaagaaata cgtttgcaaa ttcatgccaa ttggtaacac cctgccctgc
     2881 ataatggtaa agcccccaat agtcaaagga agaatgtctg attttctgta ctatttcaaa
     2941 tagtactctt gctatatcgc gagctgaagt aggccttccc gattgatctg aaacaatatt
     3001 caattctttt ctgctggaag ccaaatttaa aatcgttttt acaaaatttt tcccttgttc
     3061 accaaatacc cagctaactc gcaaaataat atacttttta agttgggata aaatagcctg
     3121 ttctccttcc catttcgtgc gcccataggt acttactgga tgcggaatat cggtttcatg
     3181 ataggctccc tccttttctc catcaaatac ataatcagtt gaaagatgga tcaaggggac
     3241 gtttttcaag gtacaataat tcaccaggcg acagacaaat tcggcgttca caatatgagc
     3301 caaatcaggt tcatcctctg ccttatctac agccgtatag gctgctgcat taatgataag
     3361 atccggcttg atttccgata acacatcatg aacccgttcg agtttggaac aatctagaat
     3421 atctctggta caagcatata cttcatgctc agaggaagaa aatcgcttga taatctcggt
     3481 accaacttgc ccattagcac ctgtaaccaa tattttcatt taggcttgat atctcggtaa
     3541 aagttcagca tctattaaat ttaaaagctt ggctgcagca tcttttggag acaattgaac
     3601 ctgctctcct aatggccaat caattgctaa ttgcggatca agataatgaa tggaaaactc
     3661 actttcagga tgataatagt cagtacattt ataagcaaaa tcagccatag cactcaaaac
     3721 ataaaaccca tgggcaaatc ctttaggaat ccaaaattgc cttttgtttt ctccggaaag
     3781 aatcactcca acccattgcc caaaagtagg ggagcctaac ctaatatcca cggcaacatc
     3841 aaacacttca ccagctaaaa ctgaaaccag ttttccctga gtttgttgac tttgataatg
     3901 caaacctctt agtacacctt tttgtgatct tgaaaaatta tcctgtacaa aacgatcagt
     3961 tattcctaat aactcttcat atcgtttagc ttgaaaagat tcataaaaaa accccctttc
     4021 atcaccataa atctttggtt cgataatttt aagttcatcg atctgagtat ttattatatt
     4081 catatcgtta ctaaagctct taaaagagaa ttataattcc ttaatattta aatcttttac
     4141 tacctgtatc cagttttcac tagaacaaat aattaagcta cctcaaaatt gttttgttta
     4201 tatttaataa aaatatcatt caaacaatga atgactctat caatgtctct gggggttaaa
     4261 tttgacccgg aaggaagaca aataccatga tcaaacaagt aatctgaaac gctgaattgt
     4321 tcatcatgag gatagtattt agagcctgca aataaaggct gtctatgcat aggcttccat
     4381 gttcttcttg cttcaatgtt aaatggcttt aattgctcaa tgacttcttc cggttttatc
     4441 ttgtttggcg ttggtttaat aaccaatgtc gataaccaac gagtactgta tccttgggtt
     4501 atctctggca tcatgtcaat aaatgggtaa gattccaagg cttgcttata tcgttcaaaa
     4561 atagctcgtc ttgccttaac ccgtttttcc aatactttca gctgacctct gcctatacca
     4621 gcaagcacat tactcattct gtaattatac ccaaccacag tatgttcata atggggcatt
     4681 ggttcgcgag cctgagttga taaaaatctt gctctttgta tgagactctc atcttcagac
     4741 accaacatgc cgccaccaga cgtggtaatg attttgttgc cattaaatga aaacacgcct
     4801 aacttgccga aagtcccgct gtacttgtta ttataggtgg ctcccaaaga ctccgccgca
     4861 tcttctataa ttggcacatt gtattcattg cataattgac ataaagcgtc ataattagca
     4921 ctttgtccat ataaattaac aataatcaca gcttttggca tttgattttt tgctttactt
     4981 tctcttaatg cccgctcaag agcaactgga gacatattcc aggtgtccaa atcagaatca
     5041 ataaataccg gagtcgcacc ttggtataag ataggattca ctgtggctac aaaagtaaag
     5101 ctggatgcga acacaatatc tcctggtttc acatcgagta atacgagtgc cagatgaatt
     5161 gcagctgttc ctgagcttaa agccacagct gattttatat taatgtattc cgccacttct
     5221 ttttcaaaag aatcaacatt ggggcctaaa ggggctaccc agttggtggc aaaagcttcc
     5281 tgtacgtatt gctgctcatg ctcaccgata tgaggcgagg aaagcagaat attggaagag
     5341 agttctctcc ggtggatcaa gcgtatgggg cgattactct catcgataac gggaatatga
     5401 tctaattcat attcctgcat caaatgataa gcatcctgaa tcgtagcaga gacaggtaaa
     5461 gcttttgatg aatgctctga taattcctct aaagtagaat ctaatgtaaa tccttttaat
     5521 agcaatcttc ggatgtcacc atctgtaact gtccttatta aatgttcatc actatctacc
     5581 agaaataaca caccttgagc tgtagcatcc agcttgctca aagcagtttt cagattttct
     5641 tgtttttgta tatataactc tttgatagta atcattttat tccttttaaa ctgcgggtac
     5701 gcctttcaca acagcatttt cttttacatc cttcacaacg actgagccag ctccaattat
     5761 tgctccatca tctatagtca caccaggcaa aacaactgct ccagctccaa tcagcactct
     5821 ctcaccaatc ttgactcgcc cacctaaggt gctattaggc gcaatatgtg aacacgagcc
     5881 aactatcact tcatgatcca caacggcact gtgattgatg atacatccct cacccacctg
     5941 acattcaggg ccgagtattg cctgtgctgc aataaaggag cctgcaccga gcctcgctga
     6001 tttcgcaata atggcagccg gatgaataat tgtaaacaag tttgcattgg aattcatcaa
     6061 atctataaca ttctttctgg cctgattatt tccaatgctg acgtgcacat aaccaatata
     6121 gtcagctaaa ctgtccatag ttgaatcaac caatactcca cacacctcct gacccagcaa
     6181 actcttattg ctatcacaaa gtgaaacttg taaagagtgc tgagtcagtg ataaagcatc
     6241 aatcaccacc ttacagtgtc caccacatcc tattattttc aagttagtat gcattgcatt
     6301 tattcaatat ttgtgagtgc aaagggatag tttttaacaa ttgataacac ctttcactcg
     6361 tttttccatc cccgtaacaa ttagagtatt ttatcttggg tttatttagc gcttctctta
     6421 gaccagtcaa aatagcatca taagtaacat caacatcaat gacattgtcg cttcgttctc
     6481 ttaaattttg cctgcttcca acattaacta cgttcaggtt aaatgaggct gcctctatga
     6541 tgccactact ggaatttccc agcatcacat cagaatttgc aagacaatca ataaattccg
     6601 gacgatgtag gtgcttgata attctaacat caggatgatc aatatattcc tgaatcactt
     6661 ctcgaattaa atgaccaccc gtatctgaat tcggctcaag gcaaataatc tgcaaatttg
     6721 tagcgagtgc tgcctgaatc acgctttgaa attgaatttt aatcgagtca acttcttgaa
     6781 caaccgggtg atagattaat aaacaaattt ttttgtcagg atgaaatcca taacgttgat
     6841 taaacacatc acgtgtagac gttttatact gcatgatttc atccaagcct ggagcaccaa
     6901 cctgaaaaat ggtttcttcc ttctcaccca ttctaatcaa tcgttgtttg gatgcctctg
     6961 ttgcgacaaa atgataatga gataatttgg aaatcgcatg ccttaccatt tcatcaacgg
     7021 ttccagagcg ctctcctccg tgcagatgta caaccgggat atttaaatgt atcgctgcta
     7081 tagctgcggc caacatttca cctcgatctc ccagcaataa aacgacatca ggagtttcac
     7141 tttcgaatac ctcggtgaat cccaaaagct catggccgat agcttttgcc atagaactat
     7201 gttgggcatt gtcaagatca acaggaataa tgccgcatat tgagaactga tctgctttaa
     7261 tttcgttaac tgtatttcca tacaaagcat caagatgcat accagtgaca caaatcgata
     7321 agtcaatgtc ttctgactgg tgtaatcttt ttagtacttc tctcatcagt ccataatcgg
     7381 cacgagtacc tgtaacataa attatttttc tggtcattac gcttcaatat gttcccataa
     7441 caaagtagta cctgcagaca aattcatact taatcgagcc cctacaatat ttgatatttc
     7501 tgaaggcgct atcccggtac cgggtcttag caagatcaaa tcttccttac tgatttgtgc
     7561 tcctttcacc aaatcacgtc ttaaagtaat acttcttcta accagagctc ttatcggcag
     7621 ctcattatcc gacggttttt tttcaccact tcccaaaaca gtttcagcgt ctctgatgga
     7681 ctgcactaaa ttcttcattt cctctggatt catagaagca agatgatctg gaccaggaag
     7741 agatttatcc attgtaaagt gcttttcgat aacgcacgcc cccattccta ctgctaatgt
     7801 tggcaccaaa atacctaaag tatgatcaga ataacctact ggcaattgaa attcgtcagc
     7861 taatgtctgc atggctttca aattcacgtc ctgataagct gccggataat tagaggtaca
     7921 atgcaacaaa accagataat cggataaaga gtttccatag tagggtttta ctgtatctat
     7981 ggctactctt acttcttgta gatcgcacat ccctgttgag atgattaatg gtaacttctt
     8041 actggcacaa tgctgcaagt aaggtacatt agtaatttcg ccagaaggga tttttaatcg
     8101 ctttacccct aaagaaataa gaaaatcaat gctttgttca tcaaaaccag ttgacataaa
     8161 ttcaatgccg agtgaattgg ctaattcact tagtaaataa tggtcctctt ctgaaagctc
     8221 cagtgccttg agcatctcgt actgggtaga ggattccggg gcattatttt tttggtattc
     8281 cgctttttct actgttttat tgaccaaggt gtccgctttg aaagtctgaa actttactgc
     8341 atcagctcct gactctttgg ctgcataaac taattcttta gccagttgaa gatcaccatt
     8401 atggtttact cctgcttcag caataataaa acaagtcata ctagagcctc ttggtttaat
     8461 tccattaatt tttctgttag agcccaatcg attggcgtat cgatgtcgat ggattcacta
     8521 atactattta acaataaagg tttggttggt tcgctataaa aactcttatt ttcgataatt
     8581 tgtttagctg ttgcaatata aattgaccca tttaatttat aaattggatt ctcgttgcaa
     8641 taaatttcca gatcctgaaa aagctctggc ttaaccaaat tgccttggct atcaatgctt
     8701 ctacaccaag aaggctttaa agagatagga ctaactgaaa cgacactttt tcccgttgct
     8761 tgatgtattt caacagcatg tcttatggtt tctggcttcc tgaatggaga agttggttgt
     8821 aaaagcaata cactgtcaaa aaaaacctcc atttgctgaa acttaaacag gaggtcaata
     8881 acagtatgaa tcacatccga agtatccgtg gctaaatctt ccgatcttag ccaaggtact
     8941 gaagccccat attgaacggc gacgtctgct atttgtttac tatcggtcga aacaacgatt
     9001 tcttcacaac aagacgactg caaggcagca acaatagtat gtgcaattaa tggctttccg
     9061 gcaagtaatc tggtattttt accgggcagc ctctttgagc cagcccttgc cgggattact
     9121 gccaatattc tcatttttaa tgcaccctaa atctgcaaaa actgttgaat gatgtttaaa
     9181 cctacttccc cgcttttttc aggatgaaat tggcatccgt acatattttc atcttttatt
     9241 actgcgctaa tagcctgacc atcatataaa gtatcagcca gacgcttttt aggattacta
     9301 ggcatggcca taaaagaatg aacaaaatat actgaagaat ttaatggtat atttttcagg
     9361 atagtatgac accaatcctc gccttccgag gtggaaacca gttcattcca gcctatatga
     9421 ggtattttat gcaattgacc atcaactcca tgtgaaggaa cgctaacaac ctcgccagct
     9481 attagatcca ggccttcatg ttgtccgaat tcagtacttc ttgagagcat catctgcatg
     9541 ccaagacata ttcccagaaa aggttttcca ctccgcgcaa attgctttat gggttctata
     9601 aaatttaatg tagttaatcc tttcatccca tcttcaaaag cgcctactcc tggcaaaacc
     9661 aaccgatcgg cactcataat ttcctcgggt gtgctcacta aattgacaga agcatcaaaa
     9721 tattgaaaag ctcgagccac acttaataaa ttgcctaccc cataatctac aattgaaact
     9781 gaactcataa gactctcaca tgaatgccat tatctaaagc atactttctg atttcaggta
     9841 aacctattct tttataatgc aaaacatccg ccatagcgac tgcatcagcc ttagactcac
     9901 aaaccacttc agttaaatct tccagcttac ccataccccc acttgcaatc accgggatag
     9961 aaaccatgga agacacctgt ttcactaatt ccagatcaaa tcccttacgc gtaccttcct
    10021 gatcaataga ggtcagaaga atttcacctg caccaaattc ttcgccattc ctagcccaat
    10081 caacaacatc cattccggta tgttcacgtc cattgtctgt catgacctcc catcttccat
    10141 tcacagtgcg ttttgcttcg atagataaaa caacacattg agacccaaat cttctggcaa
    10201 catcagatat tagagtaggg tttttagttg ccgctgtatt aatagctacc ttatcggcac
    10261 cgcaacgcaa taattgcttg gcatcatcaa cactgcgaat tccgccacct acagtaattg
    10321 ggataaatac attttcagca gtagtcttta caatctctga caaattatta cgcccatata
    10381 aagatgcaac tgtatccata taaatcaatt catctgcacc ttgtgcataa tatgccatag
    10441 catattcatt gggatttcct actactctta atccctccag atgcactcct ttgattaggt
    10501 taggtccttt aatatctagt cgtgcaatta gacggacatt actcatcaaa cagtctccaa
    10561 atccagatat tcagctttag ttttttcaag atttgttact tgatgtctaa gtttccactg
    10621 ttctccctct tttttccaca agtgaggaga tctgaaggta tccgccaaag ccataaaata
    10681 ggctctgtcc ataatcggtt gctcgaacat ttgcgaagca atgggaaact ctttggggtt
    10741 aatactcaag tatttaaaaa tttcttcagc aaaacgctct ggaaactctt gatcaaaacg
    10801 cttcaccaga gcaacacctt cttcgcgagt aatatccccc gaacgtattt cctgggaggc
    10861 atcgtatgtt gctcttccaa taccgaattt ggttagcgtg gtgtagtaat ggaaatcatc
    10921 aattttgtca tcaatactat tgtatttact gtaggttcct ggagttcgtt caggagaagc
    10981 ttcaaaaccg ccatgttcca cagaataata ataacaactt tgaggatgcc acttcagata
    11041 ataacccaag taatgaacct ctacctgctg ctcttcgatt tgctgtggat cagcaggtag
    11101 gtaagcatcc aaatcattgt catttaaacc gaaatcgctt tttaactctt gaacagatgt
    11161 accacctaaa aagattttgc ttttatcgtc cgcggtaaag tatttccagt ctcttttcgc
    11221 agattccgta tcacctattg gattaccata ttccgcttca ttttctccat aaacaaccaa
    11281 tttaatttta tgcaacaaag ccattttagg agcataagct ttttggccaa tcataaaggg
    11341 ttgaaatgga tggaatagat tctcaactgc aagtcgtgtc aatagcctgt gaactctgcc
    11401 atttggagta aacagatgat tatcaaatcc ggcatgtatc caagattgaa aattcctcca
    11461 accccatggc gtatacatat ggggagccca agtcacggtc aagggattca ttccgtattt
    11521 atattttaaa atgtgggcag cataaaaact atcctttcct ccggaaccag gaacaacgca
    11581 atcataagaa ccatccttgc ttctaaatcg atcacaaagc tctctcaatt gcctgtctcg
    11641 ctcttcccaa ttaatagtag attttttgcg ttcggcaact cgacaggcat cacaaatgcc
    11701 ttcgtcatca aaatggatgg tatgtttttt tgattctttc ttgtgctcat actctacagc
    11761 agaattaggc ctttgatttg aaataacaca gcttttgcaa aattccacct tatgtggtag
    11821 accatacttt gtttgtagct tttcatctgg gagctgaaaa cttgataaat caatggactt
    11881 tggtgcgtaa attttgctca tatcaattcc tatcctggca ttagacattt ttaaaaaacc
    11941 gcagtataca ataaaaattt caggtattaa acaaatcctg attgaagaac caaaatatat
    12001 aatttaataa atttgtgttt tcaatagtcg ccaattaccc ccttttaggg atatactgga
    12061 tgaatcgagt taaagtagga attttgcatg tccttgaagg ccatgtataa ccaaatcgca
    12121 gaaaattacg cgacagctaa tcgttttggt tcaattagtg aaagccatca cgttgccatt
    12181 gagcagatga ggaaatttca tttggggttg aaaccccatt ataaaatcct cgatctgggc
    12241 gttggcaatg gctctttttt gcaaaaattg catcacttaa tgccaatggc caatttcaca
    12301 ggaattgatg tttcatcaga aatgttgaaa cgagcgagcg aagcccttcc tttaacaacc
    12361 attgaaggca gtgctgcaga agccaacaag tttttgcccg cacatagcca ggacttggtt
    12421 ctggctcatt ttattaatgc atatatccct attaatgttc tctttgatga agccaaatac
    12481 ttgacgcgtg ccaacggtca cttctcttta atcacaacaa cctatgattc ttttcctgta
    12541 gcgcaacaac aactggccaa cttcattgct caggatacta ttttaagccg agttgttggt
    12601 cactactata aatccatagt taaaaatacg actgttgctg caaatctcga cgaactcttg
    12661 ctggcattta aacagcatca atttaatatt ttggatcacc aacgtattga aatacccatt
    12721 acccttaata acattgatga attggcactg tttggaatag agggcacctg gtttcttaac
    12781 actctatcga tacgcatgct gccaaaatat tttttaattc agcgtcttaa acgacttttc
    12841 agcaaaatct ttacttttcc ctaccatgac actcatatta ttgatgtagt tcttgccagg
    12901 aagtgataat ctttggcggt gggttagaaa aaacggaatg aggtgtgaaa ttgatttctc
    12961 aagcagttaa aaattatctt ccagaactaa gtcttcgcca aaaacaaacc aataatatta
    13021 tttttattac tttttggagc cagttttctg tctatgccct gaatacaatt ctggttttat
    13081 ttttaactcg acccttgatt gctcagggat taggctacag ccaagccaaa gcgtacgctt
    13141 tcattggtgt aactcaagcc acaggctacc tgatgcctat tttaggtggg tatatggctg
    13201 ataatattat tggtgtcaga cgatccattc ttttgggtag catcatgctg gcttgtgctt
    13261 atttactggt catgttaagt gggtacacca tcagttctct tggggatcaa ttatttatca
    13321 tagcttttgc ctttatccct gcaaccaact cgctattaat gggaaccgca tccagtatgg
    13381 tttctcatat ttattccgat gatgccatta aagccaaatc cgctatgact tattattata
    13441 tggctattaa tgttggagct ctgctggcaa ccatgatcgc gccagttcta ctagaaagtc
    13501 gttatggtcc actttcagta ctcaccttaa cctttattgg caaatcgatt gctgccttga
    13561 attttgcgaa acgttactcc atatatgata gtgtcatctg gggtatggat aaatccaaat
    13621 tttctaacca aggcctattg cgccttgttg cttatatagc ggctatctat tctcttactt
    13681 tatatgctta tactcatgtt tatatcgcca gcacactgat aggcgcagga tgtgctatag
    13741 gaattctctg gtttttaatt aaaaccatta tgcttaaagg cgaaactcgc agtaaacaaa
    13801 tgatcgctgt attactgata atagaagcta tagtattttt tatcatatac aatcaaatga
    13861 acagtacctt ggtattattt gcagaaaaca attctgatct aagttttctt tggtttaaaa
    13921 tttcacccgc tcaataccaa atgctaaatc ctttgctcat tttactgatt ggaagtcaac
    13981 ttcctcgttt ctacagattt ttttcgcgct ttacgatccc ctatcaattt gccgcaggca
    14041 caattctctc tggtatcgcc ctgctggtta tggcttttgc agcccaaaat gcaatcaatg
    14101 gattagtgaa tggtaactac attgctttga cctacatttt aatttcaatt gccgaattat
    14161 gggttagtgc cattggtctt agcatgattg gactgtattg tgatgcacgt gcgattgcct
    14221 ttgcgatggg cgtatggtac ctggccagtt ccatgtccaa tgccatttca ggacgaattg
    14281 caggttgggt cgccatacca gagaatatta attctgctct tgaaagttta ccctattata
    14341 aaaattatta tttgattatg ggcattagtg ctttggggct tggtattctg atgtattttg
    14401 tagcttatta tttacacaag attatgaaac gacgaggtat tgagttagca taacgtaact
    14461 gaacattgta atattcgaat atgcttcgag acggtgcttc gcacctcctc agcatgaacg
    14521 gccagatggg aaaatccaat tttgtaatca gccttggagt tttatgcaaa ccatcttccg
    14581 attatccata ggtttttctt gttaattgga taaatccgtt attatattaa accgttcatg
    14641 ctgaggaagt gcgaagcacc atcttgaagc aaaaacggcc cgatgggaaa atccaatttt
    14701 gtaatcagcc ttcggatttt aagcatacca ttttccaatt atccatagat cttaattgga
    14761 taaattagtt attatcttaa tccgttcatg ctgagggggt gcaaagcacc gtctcgaagc
    14821 atgcttaaaa acatagtata agggattgac taaatgtttt gggtttatat tcttcaatgc
    14881 agtgataaga gttattatac agggcaaacg gataatttgg aaaaacgcct tactcaacat
    14941 caagataaaa tgattccggg ttgttataca agcacaagat taccaattca attaaaattc
    15001 agccaggaat ttatgtctcg tgaagaagcc ttgagcgctg agagacaaat caaaggatgg
    15061 agtcggagaa agaaagaggc tttaataaat ggagactggc aagcgttatc agactattct
    15121 aaacgtaaaa attaagagtg ggctgtgttt cattcgaaca tgcttcgaga cggtgctctg
    15181 cacctcctca gcatgaacgg tttaatataa taactgattt atccaattaa caagaaaaat
    15241 ttatggataa tcggaagatg gtttgcataa agcccgaagg ctgattacaa aaagtggatt
    15301 ttcccatcgg gccattcttg cttcaagatg gtgcttcgca cttcctcagc atgaacggtt
    15361 taatggttta ataaaaaatc taatttcttt aactaataat aaaattattt atagccaatt
    15421 cgaaaaacac aagctgttca ctaacttcaa acatcaagcc ctaaattatc ccgtttgaac
    15481 actcggtcag ccctatagct tgatcgaact aatgggcctg aagccacttc aaaaaagcct
    15541 ttttttagac caatttgcct tagttcagaa aaagtttcag gagttacgta acgtgctata
    15601 ggtaaatgat ttttagttgg ttgcaaatat tgtcccaaag tcaaaatatc cacgtgatga
    15661 gttcggagat catccatagt ctgtataatt tcctcatcgg tttcacctaa tcccaacatc
    15721 aaactggttt tggtcagaac atcaggtcga tatttcttgg caaaagccag tacattcaag
    15781 gtttgctgat aacccgctcg attatctctt accgggtgtg tcaggcgctc aaccgtttct
    15841 acgttttgcg caaacacatc aacgccacta tctagcaata cagcaacatc tctttcactt
    15901 ccttgaaaat caggagttag cgcttcgact ttggttcggg gagatcgttt tttgattgct
    15961 ctaatggttt tggcataatg attcgccccc ccatcaggta aatcatcacg gttaactgaa
    16021 gttaaaacca cataatccag attcattaag gccacagttt ctgcggtatt ctcaggttct
    16081 tctgcatcaa gccaaccatg cggatttcct gtatccacag aacaaaatcg gcaagcccgg
    16141 gtacatacgg ctcccatcaa cataatagtt gcagttccat gagaccaaca ttccgagata
    16201 tttggacatt tcgcttcttc acaaactgtt gccaagcgat gtttttgcac ttgttcttta
    16261 acttggctat aggctggggt tgtatgattg actatacgga gccacttcgg ttttggcaat
    16321 cgctcatgat cttgacctga tgattttatt ccatctttaa tagctgtcac accttgagat
    16381 gtcttatatt tttgtccact ttccacaacg attggaatat cgatcaattt gcccatgacg
    16441 ccatccgaaa tacaaagagt tatgatgatc ccaaatcata aagataagat caagttcatt
    16501 taaaaatcgt tatcagagga aaatcatctg ttaccatgga tttactccga aaatagtata
    16561 attaaaaatt gttggaaata tattccacta taatatttct accctgttcc ttcctgcatt
    16621 tttagcttta tacatggcat tgtctgcccg cataaataaa gattcaatat catctttttc
    16681 ggttaccgta gttagcccaa aagaaaccgt aacatcgact tttttctctc gataataaaa
    16741 caaacaatct tcaactaatt tacgtaattt ctccaaaatt ccaaaagctt ccttgctgct
    16801 agtttgttca aagataaaca caaattcctc accacctatt cgagcgataa aatcaaccgt
    16861 cctgatcgat gatttaaaaa tcatagctac ctttttaaga accttatctc ctgcaagatg
    16921 gccaaaatta tcattaatac gtttaaaatg atcgatgtca cacacagcaa gagacaatgc
    16981 gcttgaactt cgtttccagc gctgaaatga gtccagaata tgctcctcat aagattctct
    17041 attaggtaaa cctgttaaac tatcgtggtt aattctgtat ttctgaaaag caagcaaatt
    17101 ttttatttct tccgcatttt gctcggattc ctgtaatcta gcttgcaact cggatacttg
    17161 ctttgcatat tcttcctctc ggattttttc attgtgtcga tattctttta acctgtcacc
    17221 aatcattttt atattttggc tcacctttga agataattcc tcaatcgttt tagaggtatc
    17281 caaatgattc ttgatttgat caagattatc ctgaattcca cactctaata aacgactgtc
    17341 ttcacttgcc tttttgcgag catctcctga tactcttaaa tagatttcaa aatcattgag
    17401 ctgatcagtt aactgttgaa gaaacccttt gaatcgattc tgctcaacgt taaatgactc
    17461 aacgactaat tccgtcaaat tgtcaataat agtgctaagc tcttcatcgg tgagtgcatg
    17521 ttccaaattt gtttttatga ttcccctctt ggaatccaag tcctgaggga tagataagtg
    17581 gttaagtaat tgttgcaagc tgtcatttac ttttaaacta atctcagatt gataactttt
    17641 ggaggggtta tcgacttttg taatttgtgg taactcctcc agctccttaa tcacagaaga
    17701 aatacattga gttaagactt gattaaaacg aatcaaaatc gcatggcttt ccatctcggt
    17761 atccagcatt ttttgtaatt ttgtaacggc tctcttatcc tgagatttat tggcaatacg
    17821 accaatggat tccacgccct gtttgattag tccattgact aaacgagaat tttctgcttt
    17881 tttcctttgc attttggaaa gtatttctgc caaacgtttc actttttttt ctatgacagc
    17941 aggttcaaat tcagtatcca aataatttct tagttgcgaa aggcgatcat ccaactcata
    18001 attaattcca atgggattaa taatcaattg attaatcaca tttctgagcg tattaatttg
    18061 attttgcatc ttgtcacatt tgtgctcata ttggctaatt gtgttaacaa tttttttctt
    18121 taattcttga tcaatcattt aagacttcct ttcccgttca tttattttgc ctttttttaa
    18181 ccacttcaag caaaatcttt gcatgcgatc catcacaaaa aggagggctt ttggtttgtt
    18241 tgcaattgca aaaataaatg tcttcagtca aatctgctat aaaagagact gctttatccc
    18301 cgcaattctc tttatcacag aaaggctggg tcttgctttt gccacaacca caccatacgt
    18361 aagtctttcc ttctgttacc tccacagcta ttggaaataa atgcttaaac tctgaattat
    18421 tatcatccat gatgagagtt tctccataag cgatcagctt ctaagcctgg ctcattttta
    18481 attgtatgcc ataaaatgaa agatattcag aatttataat tattttatgc cgattgaacc
    18541 ctcatgtcat tttagaagca ttaaaccaat tcagtaacag gccggagata cccaatgaaa
    18601 caattaaaaa taatgataaa gccagattag acaaggcata gggataaatg attaata
//
LOCUS       DBNCWK010000053        18433 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.053, whole genome shotgun sequence.
ACCESSION   DBNCWK010000053 DBNCWK010000000
VERSION     DBNCWK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18433
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.053"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            24..731
                     /locus_tag="AC06IO_001596"
     CDS             24..731
                     /locus_tag="AC06IO_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIO9874783.1"
                     /translation="MNKVITESQKFTLRNVANMLFCVESSVLPAQSNIAQKIQTKGIM
                     YNDLCKDGFDTEAPLNSNSLSIAGLTLVELLVTLSVFAIILTLIVPSLRTMILNNRLT
                     SNIDSLVSSLNYARGVALDRAVNVAVCPLGSPGSTACGANWSSGWIVVTQPVAGAPTL
                     LKSHQTSVNDPVITSNVSSVIFDPHGLSTTQSNFTMCDNRGNAFARSAMVLATGFVQS
                     GTTPGQAVWNNGALNCP"
     gene            744..1283
                     /gene="pilV"
                     /locus_tag="AC06IO_001597"
     CDS             744..1283
                     /gene="pilV"
                     /locus_tag="AC06IO_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HIO9874784.1"
                     /translation="MINRMDTLAKDKGMTLLEVLISVVILAVGMLGIASMLIVSNKAN
                     NSSYAKLQAVQAVYDIFDRIRANSDAASNGSYNVSNIGSNGYPTSVTAPSVLCNATVC
                     NPNQLAQYDTWYWLTRVVSQLPNGSGSITSALSGVAGNTVITVTVQWDDSKAQNELGA
                     SSASSGTANFVQFRVQSQI"
     gene            1280..2347
                     /locus_tag="AC06IO_001598"
     CDS             1280..2347
                     /locus_tag="AC06IO_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIO9874785.1"
                     /translation="MNIKYRLQQGFSLVELMIATLVGLILTYSILQIYLAQTQLYKAS
                     NSQDLIQSTENAISNLLIPMIRSAGFAGCGSIITAVSSLNAGGPRPLGILNTTPTILM
                     GYNGSGASFTITQSNPANSTNASHWSPALDSTLVGNAQQGSDVLVVLGSTSDSYPTGI
                     TTIEPGSNTFVVQGTSGMSLTSGMFGAVSDCGKTVIFQITGVAGTTLTHDAGAGVLQN
                     ASSAFPVNFQTGSQFIPIQQTAFFVGQGQGGQSALMRASLVGNSWTIQPLVPGVEIMK
                     VQYGIGNNGVITRYVSANAVTDWAQVYAIRLGFLIAGQLGSGSLTTRQYNVLDTQITV
                     PADNRLRHVFELTINLRNSIS"
     gene            2344..2856
                     /locus_tag="AC06IO_001599"
     CDS             2344..2856
                     /locus_tag="AC06IO_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIO9874786.1"
                     /translation="MNVSSTSQYSKQRGYVLITSLVLMSMLTVLALTQISQNTSQTRI
                     ATNATDSEISFEKTEGAINEAINLIINGTYNPQSFLASNNGMYLFDQSATTPLWKTIN
                     WSSGSAVINSFQGNTGAQASYFLEKLPSVIMPGQSMKKPSNVYRITARSSGASGDTSV
                     LIQSTIQIQQ"
     gene            2869..6378
                     /locus_tag="AC06IO_001600"
     CDS             2869..6378
                     /locus_tag="AC06IO_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HIO9874787.1"
                     /translation="MDALTFWNLTRKGSQKGIKILPRTLMAILLSASVSLYAVPLAIP
                     QIPLIIASPVHPQVLILLSNSQSMDGNLSGAIMTGSGSLSSGLSSLYNSSSPVNYTVP
                     AGFTPPVQGPDANGLAPYTVVQGGNKVDNSASRLNVAKAGIQAIIENYMPTTDFALGT
                     YSTSNISSYNTWVYYMSPPGSDFVFTNTPVAGNRYVTNPCYNYGSASSTVSSNCSSIG
                     NLYGTTLVSSSQYLQIGDSSDDPDINDVLYAGSGFPGIFVSYNGPTPSSPFPPNYTIS
                     NYNQGNIRISYANTRPSIGNFSSSPTNAGFVPFSQQVMYVQRGFGYYSNQSYATGNML
                     VNMQTAGTNPTTTSVNNAINAFLPHLKPETNSTATTEIKAAAVQSPLAGLLTRSRSFM
                     KTVGTTSGNCPQKQYIILISDGLPTQDLQSRYWPPLGSAAATGYGVTATFNADGSLNS
                     TNSQALSDAINEIKALKNDGVLIFIIGMGAGVDPAVNPEAAATLRAMAVAGGTENYYP
                     ATSPETLVSSLNSILSNIQNGSFSTSAAAISSTRIDSSTVEYQANFITRDTPYQDWTG
                     NLTAIELDKDTGEPTNSIQWSAQSTLDSLVSGTGWSANRKIATWDPIAADGIPFRWAN
                     INATQQAQLQPSDLLGEKRLEYLRGNATLEKRNGGTFRDRSHVLGDIIDSQVIYVGPP
                     SAPYLTASYISFAKANANRQPMLYVGANDGMLHAFNATTGEEFFAFIPNALFNNLHQL
                     TSPLYNQNHLFFVNGSPESADVQFSDGSWHTILVGGENAGGKSIFALDITNPTNLSSE
                     AGVANAVLWEFTDSDLGLTYSKPQIGQIKSGSPSSLNFAVFFGNGYNSTNNTSVFYAV
                     DPQTGAVLRKIDLCAAVPSACNVNLPQGLSTVALGQKDGLQADPVTVVYAGDLQGNLW
                     AIDVSSADPASWSARVLFQARDSAGTPQPITTPPLVTLNPNYPRNPGLFVVFGTGQLL
                     TTSDLVTTQRQTIYGVWDKPLSSVPYVRANLQQQTLTLVNSATSGLSTNIITATAATI
                     NWSNKVGWFADLPIAGQRLITTPELINGAFIATLNTPPLNSCGFGFTSMLLELNFLNG
                     GAFQYARFDISGDGGFDIADQYNGAYPVGIGLSNSYANSPTVLGPNKDNNLVILITQS
                     DGTQTTVIAPNITPRKIGWWQIQ"
     gene            6390..6839
                     /locus_tag="AC06IO_001601"
     CDS             6390..6839
                     /locus_tag="AC06IO_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIO9874788.1"
                     /translation="MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQY
                     IQKSRRADAHATLTQDQIILERCYSQNFSYAAACGALPAFPQTTPNGYYTINISNLTA
                     TTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG"
     gene            6917..9124
                     /locus_tag="AC06IO_001602"
     CDS             6917..9124
                     /locus_tag="AC06IO_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIO9874789.1"
                     /translation="MEIFCFLIGVLYAYTYNIYLTIAIPLLFYITPRYPIVLFFIAGC
                     GFALIHQWIVSPKGMPDTQVITQAQLEGTIASIPMQRPNKTQFHFAIEKINNNPAQGL
                     IQLNWYNKPPVLRAGQRWQLSVKLKKPRNFHNPGGFNYVSYLGARHIHWTGYIRYGNN
                     KLISNQPPSFSWLTLREKIGDALTKMAPNTETAGIIEALTLNVTHHISSDHWNLFRRT
                     GTIHLFGISGEHIALVSGIFFIVMRWIWSINYRCCLFLPANTIASIGGLLAALPYSFL
                     AGFEPPVQRALIGCVLYTIYAIGKQRFTPWQIWRYALFLVLCIEPHAVFLQGFYFSFL
                     AVACLLLTHQRWPLKNYKKTLALQLSCLIGLMPLTLHWYSYGSINGFLANLFAIPLVS
                     FLIVPLALMTMITSPFHWSWILMKPLAWLVALLLQGLTYVEQIAGINITWSITSVTMM
                     LAMTAGLFLRVLLPVKPFKHLAILWIILPLFPPRTAIYPGEALINILDVGQGLAVAIQ
                     TQHHVLLYDTGDSYFQGSDLGQMAIIPFFRSIGIKTIDKIVISHPDKDHKGGLNSLEK
                     EMQVNQLIVNDPYFYKRGSNCHSYPQWQWDGVSFRFLPIRESFNKKNNNSCILQVSNS
                     AGRVLLTGDIEKAAEEYLVRNYGSQLASEILVVPHHGSKTSSSYRFLLEVSPLYAIAS
                     LGFDNRFKFPHSKTLQSMKTLGITFFRTDECGMAEIKLPASGKINKPTCMQAE"
     gene            complement(9150..10457)
                     /locus_tag="AC06IO_001603"
     CDS             complement(9150..10457)
                     /locus_tag="AC06IO_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIO9874790.1"
                     /translation="MHTQRGLEVTTDSIEAVDSINYFHEQVLSSGQNAIQILDSAKKH
                     TDNLLIQTYAAAFYLYAQEDIANEQASDYLQSASRLLTSANEREKLIFEAVTNWSKRD
                     YANAISLLVTLLERYPRDTLALKFMEWLFYCTGQAFNAEYFLKVCDKCAPENQDESHF
                     LAIHSFALELCGQYSKAREMAEEAITMNLLTPWAHHTLAHVHLLTSDITGGINRLRDL
                     QKTWEDILPLLKGHNTWHLALFYLANRNEEEVKKLYPHISGALPDTVLEQLDTISLLW
                     RMDMAGLPQDRLLNQVVDHLSTHPLEYYTGFTNAHFIYCLVKSGYKNEADDSLKRMKF
                     YACSPSSDALWGDVVLPLCQGIYAFADADYKTALMLMEPVIGECAQLGGSDAQIELFF
                     QTYLLVLIHTKQKDKALQFFTEHLKYYNNTPLSDWWFQSANKA"
     gene            complement(10659..11036)
                     /locus_tag="AC06IO_001604"
     CDS             complement(10659..11036)
                     /locus_tag="AC06IO_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874791.1"
                     /translation="MMKKINLFKLLMIYVLGASALQAAGLSTQRIEQLSNARVTVWKT
                     IIYPSSKQVLPMHRHDNDRVLVALTDGTLKITNNKGKVHYLKLKKGKSYYLTKDVPNE
                     LHNDENVTNHPIQVMVIQLNDKA"
     gene            11173..11565
                     /locus_tag="AC06IO_001605"
     CDS             11173..11565
                     /locus_tag="AC06IO_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874792.1"
                     /translation="MLTTFLATVIGWYLVIVSLFLLFHTEHAKSAMAEIIAHRGSQLI
                     MAIVTLITGLLLVVSHNIWVMGWPVVITLFAWLVLLGGLIRFLCPDTAVRVGQSFVDN
                     PMRLKIAAVIFFIIGLFLLFNVYYVYLK"
     gene            11691..13619
                     /locus_tag="AC06IO_001606"
     CDS             11691..13619
                     /locus_tag="AC06IO_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874793.1"
                     /translation="MTRAQTCPHQTTVISSLKIIPLILGWLLKSALLIIIIAVFIPLS
                     PKMPAPGLDASWAVGLNQAVAQGLAFGKDIFFTLGPYSSIYTKFYHPATDFMMVGGSL
                     YLALCYWIAIILLMQGIKWRWSVAFAILLFSMIYARDSLFFSYSLIIGLLIHKEISGK
                     ETPLKSNFCTLFAVLFTPFGLFPLIKGSLLILSIITMLLCSFFLSLYKRRNLAVLCLA
                     SPVVSILFFWIASGQSIFNLPAYVSNTILLASGFTEAMATEGDTKELIFFGITSSCIL
                     LSILMQKQLSSLSKAFLFCIYLVFLFLSYKTGFTRHFGHAFIAGTSVLLASFLLPYFN
                     SNSKVIVPLLALSLYTASYINGHYTKISVRDNFISTYTAAYYGLKNRIQNKNWLKENF
                     ALTMNYLKARDDFPRLPGTTDIYSYNQTSLIASEMTWSPRPIFQSYSVFTAKMAEINS
                     NYLLSENKPDNILFKIEPIDNRIPSMEDGMSWPILINHYQPVRLENNFLFLRKKLVNK
                     PVTSLVPLKTEIHTFGEQVKIPDTDKPLFVEIDIQPTLWGMLATTFFKPHQLEANLEL
                     KNGTQRQYRLISNMAKSGFLLSPFIENSAEFGLLFSQNNYLAGKKVLSFSINSKQVPS
                     SHWQTKYTIHYKTFASNH"
     gene            13780..15204
                     /gene="sidA"
                     /locus_tag="AC06IO_001607"
     CDS             13780..15204
                     /gene="sidA"
                     /locus_tag="AC06IO_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector SidA"
                     /protein_id="HIO9874794.1"
                     /translation="MSKKYRTPIRLSDKMKSLKKNLQDQLAQSKSLGEQIHAGTTAAS
                     TITTGVSHVFNVIDSFLSAYRQIPLVGGILQLVATLAKSISIVSDPEKPIAEKIISSI
                     LIAAIIALSIAAITLGGLTAAIIGTVIASTVTIMEGLGFFGKIAEKFQLANSYKMKKE
                     FIDLVAKRIEPNNDKYNNLFEIRAIELQHELEKSYPNQIEQQQLKEELNFINAVLRKK
                     NITPGNNKENDAYKLNQLYAKRAEQLKTLVDKIALINPTGKRAEQNKLLSEIKSLQKE
                     ITKTDDSIEEIIAPLAKIERDNLKSTINLPLSYSTFAIAAASTLISIAGLLVVAGVIA
                     APPIMVPIMFAVGSVMAIIGLIKWTAEKMAEIEDAQLKTEETAKHEDAVLDEALNVYE
                     QQLNLELVSPSNPARQVKNILTVHDKGPALSPTESTVSSLDCRFTLFGSSSREHTKIA
                     AQKTTDTTNEQAPTTFDPISKPTR"
     gene            15315..16268
                     /locus_tag="AC06IO_001608"
     CDS             15315..16268
                     /locus_tag="AC06IO_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HIO9874795.1"
                     /translation="MARNKKLDRFYNAQNSNTCGYDQAFSEIQDGRKRSHWIWYIFPQ
                     LENLGKSPSAKLYGIKDFNEACDYLKDPVLFNRYFELIKLIEEKLKNIPLPHLMGGFP
                     DDRKFISSLTLFQATSNYLSSKDTDSQNKYHELYETCNRVFKQIAPQGYSPCQQTELL
                     VKHQSNSPVKKTTEKSSQPPQVTIFSPKRPKPNTAHPILKDLDQYIHKRENEWSYHYN
                     FLGLVAFVYFIQDLILGTDYLNDKARHVKLTAASKLQKILDPQCHERCNLNPSERKAL
                     SDGRLGEIINKHGGLNKILTEAPGKPPKTGHDQEINISPFS"
     gene            16524..17096
                     /locus_tag="AC06IO_001609"
     CDS             16524..17096
                     /locus_tag="AC06IO_001609"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIO9874796.1"
                     /translation="MSQNITLNRCEWVGQNKPHYELYHDTEWGIPVHDDEKHFEMLIL
                     ESAQAGLNWETILKKRDGYRRAFKQFNPQAVAQMTDDELNALLTNPEIIRNRLKIFST
                     RKNAMVFLSIQQEYGSFDSYVWQFVNGAPIINRPESIRAIPATSKESDALSKDLKKRG
                     MSFVGSTIIYAYMQAIGMVNDHIVTCFRAK"
     gene            complement(17255..18346)
                     /locus_tag="AC06IO_001610"
     CDS             complement(17255..18346)
                     /locus_tag="AC06IO_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HIO9874797.1"
                     /translation="MKKYHQLLVGITLLLFGVCNYAGDTGPIENSDLFRVFIPNLKPG
                     YEFSATAFYLQPGANNLGWGVITTVLPIPSPNWQVVTFNPDYQVGFNLGGRYVFANSG
                     SDLQLNWSHLRTNDTDNVFVNPSSQWISPFSQTGTPPTPSGQITGVALLKLAHASLDF
                     SYDAVSLDVGKFINFGSKLQTRLFTGLSSAWIKEELISTFRGFSLPILSLNNTSTYKG
                     VGPRLGFNNSYNISHGFNLVGQLAGSVLYGRMQPAQYQFTGTSQQLIIAGIFVNREGL
                     ANPSVNQLVPALDAKIGLSYLYSLKQNWDLNFEAGYMGALYFNPLSSYETNTNVIALD
                     TGSLSTSSAKHTQSNFSVGGPYITVSLKT"
BASE COUNT         5666 a         3920 c         3463 g         5384 t
ORIGIN      
        1 gatcaaacaa tagggataat gctttgaaca aagtgataac ggaatcacaa aagttcactc
       61 tgcgcaacgt agctaatatg ttattttgcg ttgagagtag tgtattgccc gcacaatcta
      121 atatagcaca aaagatacaa acaaaaggca taatgtacaa tgatctttgt aaagacggct
      181 tcgatactga ggcccctctt aacagcaact ctctttccat agccggactc acactggttg
      241 aattattagt aaccctatct gtttttgcca tcatcttaac tctaatcgtt ccttcattaa
      301 gaactatgat tttgaataac cgtttgactt caaacattga ctcactggta agctcattaa
      361 actatgcgcg tggagtagca cttgatcggg cagtcaatgt ggcagtatgt ccattaggtt
      421 caccaggttc aacggcctgt ggagccaact ggagctcagg atggatagta gtgacccaac
      481 cagtagcagg agcaccaacg cttttaaaat cgcaccagac ttcagtgaat gatcctgtta
      541 tcacttcgaa cgtatccagt gttatttttg acccacatgg gttatcaaca acccaaagca
      601 acttcactat gtgtgataac agaggcaatg cgtttgctcg ctcggcaatg gttttggcca
      661 ctggatttgt ccaatccgga acaacacccg gccaggcagt ctggaataat ggagctctga
      721 actgccctta acggagatag aatatgatta accgaatgga tacattagca aaagacaagg
      781 gaatgacttt gctggaagtg ttgatatctg tagtcatatt agccgttggc atgctaggga
      841 tcgccagtat gttgatagtc agtaataaag cgaacaattc cagttatgca aaactgcaag
      901 cagtacaagc agtttatgat atttttgaca gaattcgagc aaattcggat gcagcatcta
      961 atgggagtta taatgtcagt aatattggca gtaatggata ccctacttcg gtcactgctc
     1021 cttcggtatt atgcaatgca acggtatgca atccaaatca actggcacaa tatgatacct
     1081 ggtattggct taccagagtg gtgtcgcaac tgcctaatgg aagtggttca ataacttctg
     1141 ctctctcggg tgtcgcaggg aataccgtaa ttaccgtcac tgtacaatgg gatgatagta
     1201 aagcacaaaa tgagttaggg gcaagcagcg cttcttcagg aactgctaat ttcgttcaat
     1261 tcagagtaca gagtcagata tgaatattaa gtacagacta caacaaggat tttcattagt
     1321 cgagttaatg atagccacct tggttggact gatactcact tattcaattt tacaaatata
     1381 cctggcacaa acccaacttt acaaagcatc gaactctcag gatcttattc aaagtactga
     1441 aaatgccata tccaatttac tcataccaat gatcagatcg gctggatttg ccggttgtgg
     1501 atccattatc accgcagttt ccagcctaaa tgctgggggc ccaaggcctt tgggaatatt
     1561 gaatacaacg ccaacaatcc ttatgggtta caatggcagc ggagctagct tcacaataac
     1621 ccaaagcaat cctgcaaata gcaccaatgc cagtcattgg tcacccgcat tagattccac
     1681 ccttgttggc aatgctcaac aaggtagtga tgtgttggta gtattaggct ctacctctga
     1741 cagctatcca acaggaataa ccacgattga gcccggcagc aatacctttg tagtgcaagg
     1801 cactagtggg atgtccctta cctctggaat gtttggagcg gtctctgatt gtggtaaaac
     1861 cgtaatattt caaattactg gagtagcagg aaccacgctt actcatgatg caggcgctgg
     1921 agtgctacaa aatgcaagca gtgctttccc tgtcaatttt caaaccggct cccaattcat
     1981 tcccatccaa caaactgcat tttttgtggg tcaaggtcaa ggaggacaaa gtgcattaat
     2041 gcgagcttct cttgttggca acagctggac aatacagcca ttagttcctg gggtggaaat
     2101 aatgaaagta cagtatggta ttggcaataa tggagtcatt acacgatatg tctctgctaa
     2161 tgcagtaact gattgggcac aggtatatgc cattcgtttg ggatttttga tagcaggtca
     2221 actcggatca ggaagcttga caaccagaca atacaacgtt ttggatacgc aaatcaccgt
     2281 acctgctgat aaccgcttaa gacatgtatt tgaattgacc atcaacttga ggaattcgat
     2341 atcatgaacg tatcctccac aagtcaatat tcaaaacaac ggggttatgt cctgataacc
     2401 tctcttgtgc ttatgtcaat gcttaccgtt ttagccctga cccagatatc acaaaacaca
     2461 tctcaaacca ggattgcaac taacgcaacc gattcggaaa tcagttttga aaaaactgaa
     2521 ggcgccatta atgaagccat taatctgatc ataaatggca cttataaccc tcaaagtttt
     2581 cttgcttcta ataatggaat gtatttattt gaccaaagtg ctactacccc actttggaaa
     2641 acgattaact ggtcaagtgg ttcggcagta attaacagct ttcaaggcaa tacaggggca
     2701 caagccagct atttccttga aaaactgcca tccgtgatta tgccaggcca gagtatgaaa
     2761 aagccaagta atgtctacag aataactgct cggtcatcag gagcaagcgg ggatacttcc
     2821 gtactcatac aaagtacaat tcaaattcaa cagtaggttt tgattatcat ggacgcttta
     2881 acattttgga atttaaccag aaagggaagt cagaaaggaa taaaaattct acctcgaaca
     2941 ctcatggcta ttctcttgtc tgcttccgtt tctctttatg ccgttccctt ggcaattccg
     3001 caaatcccac tcatcattgc cagccccgtt catcctcaag tgctgattct cctcagtaac
     3061 tcccaatcga tggatggtaa tttgagtgga gcaatcatga cgggctctgg ttctctgtcc
     3121 tccggtttaa gctcccttta caattccagt tcaccagtga attataccgt accggctgga
     3181 tttactccgc cagtgcaagg ccccgatgcc aacggcttgg ctccttatac tgtagttcag
     3241 ggcgggaata aagtagataa cagcgcaagc cgcctcaatg tagccaaagc tggaatccaa
     3301 gcaattattg aaaattacat gcctaccacc gactttgcat tggggactta cagtacaagt
     3361 aacatcagtt cttataacac ttgggtttat tacatgtcac cacccggcag tgactttgtg
     3421 ttcaccaaca ccccggtagc tggaaatcga tacgtcacca acccttgtta taattatggt
     3481 tccgcatcgt ctacggtttc cagcaactgc tcatccattg gaaacttgta tggtactact
     3541 ttggtttctt caagtcagta tctgcaaatt ggtgattcca gcgatgatcc ggatattaat
     3601 gacgtgctct atgctggaag cgggtttccc gggatttttg tcagttataa tgggccaaca
     3661 ccatccagtc cttttccccc taattataca atctccaatt ataaccaggg caacatcaga
     3721 atttcctatg cgaacacaag accctctatt ggcaattttt cctcttctcc cacgaacgcc
     3781 ggttttgtac ctttttcaca gcaggtaatg tatgtgcaaa ggggatttgg atattacagc
     3841 aaccaatcct atgccactgg taatatgctg gtgaacatgc aaacagcggg aaccaatcca
     3901 acgactacca gtgttaacaa tgccatcaat gcttttttac cccatttgaa gcctgaaacc
     3961 aactccactg cgaccactga aataaaagca gctgcagtac aatcccccct cgctggacta
     4021 ctgacccgct ctcgaagttt catgaaaact gtagggacaa ccagcggcaa ttgtccgcaa
     4081 aaacaataca tcatattaat ttctgatgga ttgccaaccc aggacctcca gtcaaggtat
     4141 tggcccccac taggcagtgc ggccgcaacc ggttatggag tcaccgcaac atttaacgcc
     4201 gatggctcac tgaacagcac aaacagtcag gccctgtccg atgcaataaa cgaaataaaa
     4261 gctttaaaaa atgacggtgt tttaattttt ataataggga tgggagcagg tgtggatcct
     4321 gctgtaaatc ctgaggcagc agcaactctc agggccatgg ctgttgcagg aggcactgaa
     4381 aactattatc cagcaacgag tcctgaaact ctggtcagca gtttaaattc gattctatcg
     4441 aatatccaaa atggctcttt ttcaacctct gcagcagcca tcagttcaac tcgtatcgac
     4501 agcagtaccg tggaatatca agccaacttt ataaccagag acacccctta tcaggattgg
     4561 acaggtaatt tgacagctat tgaactcgat aaggataccg gagaaccaac caattctatt
     4621 caatggtcag cccaatcgac gcttgactcc ttagtatcag gaactggatg gtcagctaac
     4681 cgcaagatag ccacttggga tcccatagcc gctgatggaa taccatttcg ctgggcaaac
     4741 atcaatgcaa cccaacaagc acaactacaa ccaagtgatt tattaggaga aaagcgatta
     4801 gaatacctac gtggcaatgc taccctggaa aaaagaaacg ggggaacatt ccgtgatcgt
     4861 tcgcatgtat tgggagacat tatcgatagc caggtaattt atgttggccc accaagcgct
     4921 ccctatttaa cggccagtta catcagcttt gcaaaagcca atgccaatag acagcccatg
     4981 ttatatgtcg gggcaaatga cggaatgctt cacgccttta atgcaaccac tggagaagag
     5041 ttttttgcat ttattcccaa tgccttgttt aataatctcc accaactcac ctctccattg
     5101 tacaaccaaa atcatttatt ctttgtcaat ggctctccgg aaagcgctga tgtacaattt
     5161 tccgatggtt cctggcatac cattctggtg ggaggtgaga atgcaggtgg taaaagcatc
     5221 tttgccctgg atattaccaa tcccacaaac ctgagttctg aagcaggagt ggccaatgcc
     5281 gtgctctggg aatttacaga ttctgatcta gggttaactt atagcaaacc tcaaataggt
     5341 caaattaaat ccggaagtcc atcttcatta aattttgccg tcttttttgg caatggatac
     5401 aacagcacaa acaacacatc cgtattttat gctgtcgacc ctcaaacagg ggcagtccta
     5461 agaaaaatag atctttgtgc tgccgtccct tcagcctgca atgtcaattt accccaggga
     5521 ttatctacag tcgctttggg acaaaaagac ggattgcaag ccgatcctgt tactgtagtg
     5581 tatgccggtg atttacaagg caatctatgg gcaatcgatg tttcctcggc tgatcctgcc
     5641 agttggagtg cccgcgtatt attccaggca agagactctg ctggtacacc gcagcctatc
     5701 actactcctc ctttagtgac attgaaccct aattatcctc gcaatccagg actttttgtt
     5761 gtgtttggca ccggacaact tctgactaca tccgatttgg tcactaccca aagacaaacc
     5821 atttatggag tatgggacaa gccgctttca tccgtgcctt atgtacgtgc caatctccag
     5881 caacaaacgt tgaccttggt taactcagct acatcaggtt tatccacaaa cattatcact
     5941 gccactgctg ctaccatcaa ttggagtaac aaagtgggat ggtttgccga tctgcctata
     6001 gcgggacaaa gactcattac tacacctgaa ctaattaacg gagcatttat tgcaacgcta
     6061 aacactcctc cactcaattc gtgcggcttt ggtttcacat ccatgcttct ggagcttaat
     6121 ttcttaaatg gcggtgcttt tcaatatgcg cgatttgata tttccggcga tggaggcttt
     6181 gatatagccg atcagtataa tggggcctac cccgttggaa ttggcttgtc caacagttat
     6241 gccaactcgc ccactgttct ggggccgaac aaggataata atctggtcat tctgatcaca
     6301 caatcggatg gaacacaaac tactgttatc gctcctaaca ttacgccaag aaaaataggc
     6361 tggtggcaaa ttcaatagag gatacccaaa tgctcagtca tgtacatttt atgaagaata
     6421 gccgtatgaa acaatcggca tttaccctgg ttgaagttct gatcagcatg gtcattatgg
     6481 gaattctggt ttcaattgcc tatccatcct atttacaata tatccaaaaa tcccgtcgtg
     6541 ccgatgctca tgccacattg acacaagatc aaattatttt agaacgctgc tactcacaga
     6601 atttttctta tgctgcggcg tgtggcgcct taccagcatt tccgcaaaca actccgaacg
     6661 gatactatac tatcaatatt tcaaacctga cagccacaac gtatacctta actgcaaccc
     6721 ctgttggaac tcaagccaaa gataccgagt gcgccagcat gtcaattaac caggccaatg
     6781 taaaaacagc agtagattcc tctgccaatg cgcaaccaga atgctggaat cccggttaat
     6841 actgtaaaaa attctttaca caaaggattc aattcgagta gatttgccag taagggaaag
     6901 tccaacttaa tttgaaatgg aaattttttg ctttttaata ggcgtacttt atgcctatac
     6961 ctataatatt tatctaacta ttgcgattcc tttactcttt tatatcaccc caaggtatcc
     7021 gattgtcctg ttcttcatcg ccgggtgcgg ctttgccctg attcatcagt ggattgtatc
     7081 tcccaagggc atgcctgata cgcaagtaat cactcaagcc caattggaag gaactattgc
     7141 gtccatcccc atgcaacgtc ctaacaaaac ccaattccat tttgcaattg aaaaaattaa
     7201 taataaccct gctcaaggct tgatccaatt aaactggtat aacaaaccac ctgtcttacg
     7261 agccggtcag cgatggcaac tttcagtgaa actaaaaaaa cctcgtaact tccacaaccc
     7321 gggaggtttt aattatgtaa gttacctggg ggctcgtcat atccattgga ctggctatat
     7381 tcgttatggg aataataagc taattagcaa tcaaccccct tctttcagtt ggttaacact
     7441 acgtgaaaaa attggggatg ctttgacaaa aatggcaccg aatacagaaa cggcaggcat
     7501 catcgaagca ttgacgttaa atgttactca ccatatcagt tcagatcatt ggaatttgtt
     7561 ccgaagaaca ggaacgatac atttattcgg catttcagga gaacacatcg ctctggtctc
     7621 cggcattttc tttatcgtga tgagatggat ttggtcaata aattatcgat gctgtttgtt
     7681 cttaccggcc aataccatag cgagtatcgg tgggctactg gccgccttgc cctattcttt
     7741 tttagccggg tttgagcctc ctgtgcaacg tgctttaatt ggttgtgtac tttacacaat
     7801 atacgccata ggaaaacaac gttttacacc atggcaaata tggagatatg ccttattttt
     7861 agtcttgtgt attgagcccc atgccgtatt cctgcaaggt ttttactttt catttcttgc
     7921 cgtggcctgt cttctattga ctcatcaacg ctggccactg aaaaattata aaaaaacact
     7981 ggcattacaa ttaagctgcc tcattggatt aatgcccttg actctccatt ggtactccta
     8041 tggttctatc aatggatttc ttgccaattt attcgccata cctttagtca gttttctgat
     8101 agtgcccttg gctttaatga ctatgataac gagtccattt cattggtcct ggatattgat
     8161 gaaaccattg gcgtggcttg ttgccctgtt actgcaagga ctaacgtatg ttgagcaaat
     8221 agctgggatc aatatcacct ggtccatcac ctcggtaaca atgatgctcg ctatgacagc
     8281 aggtctgttt ttaagggtat tattacccgt caagccattc aaacacttgg ctatcctttg
     8341 gattatcctt cccttatttc cgcctcgtac tgcaatttat ccaggggaag cattaattaa
     8401 tattttggat gttgggcaag gacttgccgt tgcgatacaa acgcaacatc atgttttact
     8461 ctacgatacg ggagattcct attttcaggg aagtgattta gggcaaatgg cgataatccc
     8521 ctttttcagg tcaattggaa taaaaacaat tgacaaaata gtaataagcc atcccgataa
     8581 agatcataaa ggcggattaa attcccttga aaaagagatg caagttaatc aactaatagt
     8641 gaatgatccc tatttttata aacgtggaag taattgtcat tcatacccgc aatggcaatg
     8701 ggatggggtg agttttcgtt ttttacccat cagggaatct ttcaataaaa aaaataataa
     8761 ttcttgtatc ttgcaggtaa gtaattcggc tggcagggtt ttattaacgg gagatattga
     8821 aaaagcagcg gaggagtacc tggtaagaaa ctacggcagt cagctggcat cagaaatttt
     8881 agttgtgcct catcatggga gcaaaacgtc ttcatcctat cgatttttgc tggaagtaag
     8941 tccgctttat gccattgcat ctttaggatt tgataatcgc ttcaaatttc cacattctaa
     9001 aactttgcaa agcatgaaaa cattaggtat cacttttttt agaacagacg aatgcggaat
     9061 ggcagaaatc aaactacctg cgtcaggtaa aattaacaaa ccaacctgta tgcaggctga
     9121 ataaatggat tggctgaccc atgttatacc taagccttat tagccgattg gaaccaccag
     9181 tcactcaatg gcgtattatt gtaatacttt aaatgttccg taaaaaattg taacgcttta
     9241 tccttttgtt tagtgtgtat caacaccaac aagtaagtct gaaaaaaaag ctctatctgc
     9301 gcatcgcttc cacctaattg tgcacactca ccaatcaccg gctccatcaa catcaaggca
     9361 gtcttgtaat ctgcatcggc aaaggcatag ataccctgac acaaaggcaa aacgacatcg
     9421 ccccatagag catcagaaga aggtgagcag gcgtaaaact tcattctttt caacgaatca
     9481 tccgcttcgt ttttataacc tgatttcacc aggcaataaa taaaatgagc attcgtaaac
     9541 ccggtataat attccaatgg atgggtactt aaatggtcca caacctgatt taacaacctg
     9601 tcttgaggta gacctgccat atccatgcgc catagcaggg aaatagtatc caactgctca
     9661 agcacagtat caggcaacgc ccctgaaata tgggggtata acttttttac ttcctcttca
     9721 ttacgatttg ccaaataaaa tagcgctaaa tgccaggtat tatggccttt caacaaaggc
     9781 aaaatgtcct cccaggtttt ttgtaaatct cgaagtctat tgataccacc agtaatatca
     9841 cttgttaata aatgcacatg agccagggta tggtgtgccc aaggggtaag caaattcatt
     9901 gtgatagctt cctcagccat ttccctggct tttgaatatt gcccacacaa ttccaaagca
     9961 aaggaatgta tggctaaaaa atgggactca tcctgattct cgggagcaca cttatcacat
    10021 actttaagaa aatactcagc attaaaagcc tgtcctgtac agtaaaaaag ccattccatg
    10081 aatttcagag ccagagtatc cctgggatac ctttcaagca gggtaactaa caagctaatg
    10141 gcattggcat aatctcgctt agaccaattc gttactgctt caaaaattaa tttttcacgt
    10201 tcattcgctg atgttaataa cctggatgcc gattgcaaat aatcgctggc ttgttcattc
    10261 gctatatctt cttgtgcata caaataaaaa gcagccgcat aagtttgaat taataaattg
    10321 tcagtatgtt tttttgctga atccagaatt tggattgcgt tttgaccgga gcttaatacc
    10381 tgttcgtgaa aataattgat actatctact gcctctatgg agtctgtcgt tacttccaat
    10441 ccacgttgag tatgcattct cgaaatcctg tcaatgaatg aatcaatgat taaatgatag
    10501 cacaaataat aaacagaaag gacggaagca acgcgccaat tagatgatag tacggcctga
    10561 tatcacgatg acagcggtag agctccaagt actctcgtta taattgtttt ctctgaccag
    10621 aaattaatac caacaccaga ttccccactt cccgctaatc atgctttatc attgagttga
    10681 attaccatca cctgaattgg atggttagtt acgttttcat cgttatgtaa ttcgttaggg
    10741 acgtctttgg tgagataata tgacttacct ttctttaatt ttaaatagtg tactttgcct
    10801 ttattgtttg taattttcag tgtcccatcc gtcaatgcga ccaaaactcg atcgttatca
    10861 tgcctgtgca tgggcaaaac ttgttttgaa gagggataga taattgtttt ccacacagtc
    10921 actctggcat tagataattg ttcaatacgt tgggttgaca atcctgcagc ctggagcgca
    10981 cttgcaccca gaacatagat cataagcaat ttgaataaat taattttttt catcatgtta
    11041 gattactctc cattttcaaa tcaaataaca gtgcatcatc tcgcttattc ctcaattttc
    11101 ctttaacttt ccgacacata gctcacaaaa ttgctataat tatagtagga ggtcattgta
    11161 aggagtttga agatgttaac tacatttcta gccacagtga ttggctggta tttagtcata
    11221 gtaagcttat tcttattatt ccatactgag catgcaaaat cagctatggc agagattata
    11281 gctcatcgcg gatcgcaatt gattatggct atagtaacct tgattacagg tttgctttta
    11341 gtcgtcagtc ataatatctg ggtaatgggc tggcctgttg ttattactct ttttgcatgg
    11401 ttagtcttac ttggtggttt aattagattt ttatgcccag ataccgcagt tagagtaggc
    11461 caatcatttg tcgataatcc aatgagacta aaaatagcag ctgtaatatt ctttattatt
    11521 ggtttgttcc tgttatttaa tgtctattat gtttatttga aataaaggaa gagttattgg
    11581 ggtactatga ctcattctgt ttatgggaaa aggaagcgat aaagtccttg tcaattcagc
    11641 agcaaggaat gctatgatat gatgcttgat atgatatgcc tacaaaaaaa atgactagag
    11701 cccaaacctg ccctcatcaa acgaccgtta tttcaagtct taaaataatt ccactcattt
    11761 tggggtggtt attaaaatcc gctttgctca ttattataat cgccgttttt attccactat
    11821 cccccaaaat gccagctccc ggtctcgatg cctcttgggc tgtaggactt aatcaagcag
    11881 ttgcccaagg tttggctttt ggtaaagaca ttttttttac cttaggacca tattcttcca
    11941 tctatacaaa attctaccat ccagctaccg atttcatgat ggttggggga agtttgtatt
    12001 tggctttatg ttactggata gctattatcc ttttaatgca aggcattaaa tggcgttgga
    12061 gtgttgcttt tgcaatactt ctctttagta tgatctatgc tcgggattct ttgttttttt
    12121 cctattcatt aatcattggc ttactcattc ataaagaaat ttctggaaaa gaaacaccct
    12181 tgaaaagcaa tttctgcact ttgtttgctg tgctttttac gccattcggt ttatttcctc
    12241 tgataaaggg ctctctttta atattaagta ttattaccat gctcttatgc tctttctttt
    12301 tgagcctata taaacggcgt aatcttgctg tgctttgtct ggcatctcca gtagtttcca
    12361 tattgttttt ttggatagca tccgggcaat ctattttcaa tctgccagct tatgtcagta
    12421 acactatctt gctcgcatca ggttttactg aagccatggc aactgaagga gacactaaag
    12481 aactgatatt ttttgggatt acctcttctt gcattctctt atcaatactc atgcaaaaac
    12541 agctctcatc attatccaaa gcatttctat tttgtatcta tctcgttttt cttttcctct
    12601 catataaaac ggggtttaca agacattttg gccatgcctt cattgctggt acctctgttt
    12661 tgctagcctc ttttttgtta ccgtatttta attccaacag caaagtaata gtcccattat
    12721 tagctctatc cctttataca gcgagttata tcaacgggca ctataccaaa atatctgttc
    12781 gagataattt catttccacc tataccgccg cttactatgg ccttaagaac agaatccaga
    12841 ataagaattg gttaaaagaa aattttgcat taacgatgaa ttatttgaag gcccgtgatg
    12901 attttcccag attaccaggt actaccgata tttattctta caatcaaact tcattaatcg
    12961 cctcggaaat gacatggtcg ccaaggccaa tctttcagag ttattccgtt tttactgcaa
    13021 aaatggcaga aatcaattcg aattaccttt taagcgagaa caaaccggat aatatccttt
    13081 ttaaaataga gccaattgac aatagaattc cttcaatgga agacggaatg agttggccga
    13141 tactgattaa tcattatcaa ccagtacggt tagaaaataa ttttttattt ttaagaaaaa
    13201 aattagtcaa caaacctgta acatctttag tgccattaaa aacagaaatt catacttttg
    13261 gagaacaagt taaaatacct gataccgata aacctctttt tgtcgaaata gatattcaac
    13321 caacgctctg ggggatgtta gcgactacct ttttcaagcc tcaccagctt gaagccaatt
    13381 tggaattaaa aaatggcacc cagagacaat atagactcat ttccaatatg gcaaaatccg
    13441 gttttctgtt atccccattc atagaaaaca gcgctgagtt tggactatta tttagccaga
    13501 ataattacct ggcaggtaaa aaggttcttt cattttccat taactcaaag caagtacctt
    13561 catcgcactg gcaaacgaaa tatactattc attataaaac ttttgccagc aatcattaaa
    13621 acaacaagga tgcgtcaatc aaaatagtat accaagctct tctttgttga ttttttgttt
    13681 aaattccaaa caaatatctt cccaaaagga aaattaacaa gtaaaattat gtactaaaat
    13741 aattcatata gttttatata tgactttagg gttcttttta tgtccaaaaa atatcgaact
    13801 cccatccgat tgtctgacaa gatgaaatct cttaaaaaga atcttcagga tcaacttgca
    13861 caatccaaat cgttaggtga gcaaattcat gctggaacta cggcagcatc aacaataaca
    13921 acgggagtta gtcatgtgtt taatgtcatt gactcctttt taagcgccta tcgacaaatt
    13981 ccactggtag gcggaatact gcaactagtg gccacacttg ccaaatcaat ttctattgtg
    14041 agtgatcccg aaaaaccgat agctgaaaaa ataatatcat cgattctaat tgctgctatt
    14101 atcgcccttt ctattgcagc gatcactctc ggtggattga ctgccgcaat catagggaca
    14161 gtaattgcct ctactgtaac catcatggaa ggattgggat tttttgggaa aattgctgaa
    14221 aaatttcaat tggcaaattc ctataaaatg aaaaaagaat ttatagatct tgtagcgaaa
    14281 cgtatagagc caaacaatga taaatataat aacttatttg aaattcgtgc catagaactc
    14341 caacatgaac ttgagaaatc ttatcccaat caaattgaac agcaacaatt aaaagaagag
    14401 ttgaatttca ttaatgcggt acttaggaaa aaaaatatca ccccgggaaa caacaaagag
    14461 aatgacgctt acaaactaaa ccaactctat gctaaacgcg ctgagcaatt aaaaaccctt
    14521 gtagataaaa ttgctttaat caatccaact ggaaagagag ctgagcaaaa caaactttta
    14581 agcgaaatca aatcattaca aaaagaaata accaaaaccg acgatagcat agaagaaatc
    14641 atagccccgc ttgctaaaat agaacgagac aatttgaaat ctaccattaa tttgccatta
    14701 agttacagca cctttgctat agcggcggcc agtactttaa tatcgatagc cggcttattg
    14761 gtggtggctg gcgtgattgc cgcacctccg attatggtcc ctatcatgtt tgcagtaggt
    14821 agtgttatgg caatcattgg tttaatcaag tggaccgctg aaaaaatggc tgaaatagag
    14881 gatgctcaat taaaaacaga agaaactgcg aaacacgagg atgcagtttt ggatgaagca
    14941 ttaaacgttt atgagcaaca attaaatctt gagttagtta gtccttcaaa ccctgcgaga
    15001 caggtgaaaa atatattaac agtgcacgat aaagggcctg ctttgtcacc aactgaaagt
    15061 actgtaagct cattagattg cagatttact ttatttggct catcttctcg agagcacaca
    15121 aaaatagcag cacagaaaac cactgataca actaatgaac aagcacctac aacttttgat
    15181 cccatatcca aaccaactcg ttaacaaata taacaacttt ttataacttt gttataattg
    15241 atttataaat aggcagttta tgcataatac ctgattcgcg aatataaaaa ttaatgaaat
    15301 caagagaaaa attcatggcc agaaataaaa aactagatcg tttttataat gctcaaaata
    15361 gtaatacttg tggctacgat caagcattca gcgaaataca agacggaaga aaaagaagcc
    15421 attggatatg gtatattttt cctcaattgg aaaatttagg taaaagccca tctgctaagc
    15481 tgtatggaat taaagatttt aatgaagcat gtgattattt aaaagatcct gtgctgttta
    15541 atcgttattt tgagttaata aaactcatag aggaaaaatt aaagaacatt cctctccctc
    15601 atctaatggg tggttttcct gatgatcgta aattcatctc aagtttgacc ttgttccaag
    15661 ccacttccaa ttacctgagt tcaaaagata cggattctca aaacaagtat catgagcttt
    15721 atgagacttg taatagagtt tttaaacaaa ttgctccaca gggttattct ccatgtcaac
    15781 aaactgagtt attggtcaaa catcaatcaa attcccctgt gaaaaagaca acagaaaaat
    15841 caagtcaacc tccccaagtg actatctttt cacccaaacg gcccaaaccg aatactgccc
    15901 accctatcct taaggatctt gatcaatata tacacaaaag agaaaatgaa tggagttatc
    15961 attacaattt tctaggcctg gtggctttcg tttatttcat acaggattta attcttggaa
    16021 ccgattattt gaatgataaa gctcgtcatg tcaaattaac tgcagcaagc aaattacaga
    16081 aaattcttga tccccaatgc catgaacgat gtaatctaaa tccatcagaa agaaaggcgc
    16141 tttccgatgg tcgcttgggt gagattatca ataaacatgg gggattaaat aaaatcctga
    16201 cagaggctcc tgggaaacct ccaaaaactg gccacgatca ggaaattaat attagtccct
    16261 tctcataaaa ccaacagtca aggcaaaaaa tatttttgat gaggggatcc agataaacta
    16321 aatgaccgaa ttaaaaactt ttttaacaaa gagattgggg gtttcgtaag tccttatact
    16381 gttaacaagc gatttcctga ttaccttcat tacttgacaa cgaatacaac ttaatgaata
    16441 gttcacacag aatagtagac caatgatata aatactgcca ttaccaggat taaatatttt
    16501 taacccaatc taataattac attatgagtc aaaacatcac tctaaaccga tgcgaatggg
    16561 tagggcaaaa caaacctcat tatgaactat accatgatac tgaatgggga attcctgttc
    16621 atgatgatga aaaacatttc gagatgctca ttctggaaag tgctcaggca gggttaaatt
    16681 gggaaactat attaaaaaag cgcgacgggt accggagagc tttcaaacaa tttaacccac
    16741 aagctgtagc ccaaatgact gatgatgaat tgaatgcctt gttaactaat cctgaaatta
    16801 tccgcaaccg gcttaaaatc ttttctacca gaaaaaatgc gatggttttt ctatctattc
    16861 aacaagaata tggatcgttt gattcttacg tttggcaatt tgttaatgga gcacctatca
    16921 taaatcgtcc tgaaagcata cgagcgatac cagcaaccag caaagaatca gatgccttat
    16981 ccaaagatct gaaaaaaaga ggaatgagct ttgttggctc aacaatcatc tacgcttaca
    17041 tgcaagccat tggtatggtg aatgatcaca tcgtaacctg ttttcgagcc aaataaagaa
    17101 atcactcgaa ctcctgaagg tgactcatat tctccataag ctttttagcg aaatcattta
    17161 aaacggatta tctgtcgtgt cctggcctaa ccataaattg gcaatgcagt ttcatgtttt
    17221 gttaggccaa ggaattatag tttgaccacc ctaatcaggt tttcaagcta actgtaatgt
    17281 aagggccgcc tacagaaaaa ttactttgtg tgtgtttggc acttgatgtt gacaaagagc
    17341 cagtgtccaa ggcaatgaca ttggtatttg tttcataact ggataaagga ttaaaataca
    17401 aagctcccat ataccctgct tcaaaattca aatcccaatt ttgtttcaat gagtaaagat
    17461 aacttaaccc gattttggca tccagagctg gaaccagttg attgacactg ggattagcaa
    17521 gtccttcacg atttacaaag attccagcga taatcaattg ctgagaggtt cctgtgaatt
    17581 ggtattgagc tggttgcatt ctgccgtaca agactgagcc ggcgagttgt ccgaccaaat
    17641 tgaacccatg agaaatatta taactattat tgaatcctag tctcggcccg acacctttgt
    17701 aagtggatgt attatttaat gacagtatag ggagtgaaaa gccccgaaaa gttgatatca
    17761 actcttcctt tatccaggca ctgctcaacc ctgtaaaaag ccttgtttgt aatttggaac
    17821 caaagttaat aaattttcca acatcgagac tgactgcatc ataactaaaa tcaagactgg
    17881 catgtgcaag cttcaataaa gccactcctg taatttggcc agaaggcgta ggaggagtac
    17941 cggtctgaga aaaaggagag atccattgcg atgagggatt cacaaagaca ttatcagtat
    18001 cattggtacg cagatgagac cagtttaatt gcaaatcgct tccagaattg gcaaatacat
    18061 aacgtcctcc caaattaaac ccaacttgat aatctggatt aaaagtaaca acctgccaat
    18121 taggtgatgg tattggcagt actgtcgtaa tcacccccca acctaaatta ttcgctcccg
    18181 gttgcagata aaaagctgtc gcagaaaatt catagcccgg tttcaaattg ggaatgaaca
    18241 ctcggaaaag gtcgctattt tcaattggtc ctgtatcgcc ggcataattg catacaccaa
    18301 acaacagtaa agtaattccc acaagtaatt gatgatattt tttcatattt aatgaatcct
    18361 ccctaatgca tgatttctat caatcaggac ttacgaaaaa tcccaatctc tttattaaaa
    18421 aaagttttta att
//
LOCUS       DBNCWK010000054        18364 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.054, whole genome shotgun sequence.
ACCESSION   DBNCWK010000054 DBNCWK010000000
VERSION     DBNCWK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18364
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.054"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            104..505
                     /locus_tag="AC06IO_001611"
     CDS             104..505
                     /locus_tag="AC06IO_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIO9874798.1"
                     /translation="MINQQIEIFLKSPAFAVVGASSNREKYGNKVLRCYLRNGKKVYP
                     VNPHETLIESLPVIHSVNDLPDDVHSISIITPPSITEEIVKEAVKKGIQHIWMQPGAE
                     SDSAVQYGLDHKLNVISGGPCILVVMGYEES"
     gene            complement(565..1686)
                     /locus_tag="AC06IO_001612"
     CDS             complement(565..1686)
                     /locus_tag="AC06IO_001612"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HIO9874799.1"
                     /translation="MPLQPFKTSNLLTMGVELELQLISLSNFDLTAASPDILELLGRS
                     SFPGSFTPEITESMLEIATDVHEEYQQLLKQLFHIRDALVTVGDRLNIGICGGGTHPF
                     QMWSDQRIFNKTRFIEVSELYGYLTKQFTIFGQHIHIGCEDGNQALFLLHSLNRYIPH
                     FIALSASSPFVQSKDTLYNSARLNSVFAFPLSGRAPFVLNWDEFSLGYFEKMEHTGIV
                     KSMKDFYWDLRPKPEFGTIEMRVCDSPLTVERAAALACYMQALCSYLLENKEPLPHED
                     DYLVYNYNRFQACRFGLDGTLVHPKTYEQILLREDILTTLRRLKPYANQLNSTMALEH
                     IYEITHKGSDASFLREKYAEHRTLESVVNESLKQFRSSK"
     gene            complement(1688..2875)
                     /locus_tag="AC06IO_001613"
     CDS             complement(1688..2875)
                     /locus_tag="AC06IO_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIO9874800.1"
                     /translation="MIDVGKIITDLAWPISLLIAWFAGELGYRWAKLPHISIYAIVGF
                     SLASTQTGLLPSIQPPSILLLANIGFGLILFESGYRINLRWLSYNPWVAASSITEATL
                     TFFLVYILVKSFGLQTSTCLLLASLSMATSPATVVRVINEQRSSGQVTERVLHLAVIN
                     CVLAVFLFKVIIGLVVFRTSGNLWEATYASLTVTAISIIWGIIFGVVMPILLKTVKST
                     CQDNTLAFACAVILLVILTHNLKLSPVLATLTFGLVSRHRRMVFSPSQPGFGTLGDLL
                     SVLLFVFIAATIEWQRVVTGVWIGLSIITVRFVAKIIGISLFAHISGISFKKGVLTGL
                     AMTPISVFVILILEQSRYIGINLVDTLAPLAMVALTLEILGPIMVQRALIKANEISAI
                     KEF"
     gene            3205..3807
                     /gene="def"
                     /locus_tag="AC06IO_001614"
     CDS             3205..3807
                     /gene="def"
                     /locus_tag="AC06IO_001614"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIO9874801.1"
                     /translation="MALEIITIEKTENHHMLKTRAENIQFPLNREDKDLIEAMKKKLY
                     ALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIFPMHILINPSYEPLPHTRIISD
                     FEGCYSVSSKAGKVPRYDEIRLKYYDEEGNAHQQIENGFYARVLQHEIDHLNGVLITD
                     RLTPDCVQGTIEEMMVIRRAELPVEKRKLFDQIMAKKQKK"
     gene            complement(3843..4247)
                     /locus_tag="AC06IO_001615"
     CDS             complement(3843..4247)
                     /locus_tag="AC06IO_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HIO9874802.1"
                     /translation="MHKLIELQGVGPKYAEMLKRVGIESQDDLLRVCSGRRGRFRVAE
                     QTGISHKLILKWTNQADLARVNGINEEYAELLEQAGIDSIPELAHCNPEQLYSSLKEK
                     NEQCHLAQHVPGLSQVVTWVEQAKKLPRAVYH"
     gene            4434..4778
                     /locus_tag="AC06IO_001616"
     CDS             4434..4778
                     /locus_tag="AC06IO_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874803.1"
                     /translation="MCKHKNKMVRIFLLALGLFSSTTINAWYGSGITIGVARANVHYY
                     GDDIRVYDPWGYGYYDRNYYYNGVRNIIVPNVIVPNVVINVPVQRYYVPSCEEMEVCD
                     PYGQCWLEQFCR"
     gene            4980..6026
                     /locus_tag="AC06IO_001617"
     CDS             4980..6026
                     /locus_tag="AC06IO_001617"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIO9874804.1"
                     /translation="MSETIGYAAHKAGAPLKPFTFERRPVGAHDVAIDILFCGICHSD
                     LHQVNNDWAGSFYPMVPGHEIIGRVKEVGHQVHRFKAGDLVGVGCMVDSCRQCTYCHQ
                     NEEQYCKSGATYTYNSQDNHLGGVTYGGYSKHVVVTEDFVLKIDESLDPAAAAPLLCA
                     GITTYSPLKRAKVGKMSKVGIVGLGGLGHMAVKLARAMGAYVTVFTTSINKIKAGKDL
                     GAHDVVLSTDSNVMQTLANQYDFILDTVSAKHDLGQYLNLLKVDGQLTQVGLPSDPLE
                     VTMFPVILKRLSINGSLIGGIKETQEVLDFCARHGITADIELIKMHEVNSAYERLLKG
                     DVKYRFVIDMSTLD"
     gene            complement(6080..7399)
                     /locus_tag="AC06IO_001618"
     CDS             complement(6080..7399)
                     /locus_tag="AC06IO_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIO9874805.1"
                     /translation="MYNILLIFIAFLLVLLNAFFVSAEFGMVKLRATRIQAIKVNYGL
                     RGRILEQVHQNLDAYLSACQLGITLASLGLGWIGEPAFAELLGPLFVALGISSVHIVT
                     ISSFFIAFFLISFLHIVIGELMPKSIAIRQSELISIWTAVPLYWFYWIMYPAIWLLNT
                     CSNFFLKLSGMDIKNEYEGFYSSEELKLILSGQHLQSELKEDEREILEHTLDLAELNV
                     TEVMRPEDEMIIIDINEPFETIKDKILKYRYSRYPVYDSGTREMIGVIHVKDLFADLL
                     LQKNINTLKPYVRPVLKVSHKLPAMDLLRKFRAGMPHFALVYKHKSSLLGFVTLDNLL
                     HILIGRIKDEFHKTHEDWVKNADGTITTKGDCSLYSLERALDCDITLSPDEEELNTLY
                     GLIITRHGKLPNEGEIIQFVEFDALIEKVQDSFITKIKVFPKIKNQE"
     gene            8052..9347
                     /locus_tag="AC06IO_001619"
     CDS             8052..9347
                     /locus_tag="AC06IO_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874806.1"
                     /translation="MNQNCFKPKNNFVSLVMWALPLSFFTFQFLLRLWPGLTMQDIMA
                     QFSIDAASFGLIAAFYYYGYAGMQIPVALLLDKFGARFVVFAFAMLCGIGTLVFISTN
                     NFYLALSGRFLIGVGSAAGFLGVSKVVSEWFSHSQYSRMIGFSFSVGLLGAIYGGKPL
                     AHLTQNFSGQQVGLALAVFSILLGCCALLILRSPPNNTRSVTKTMNLSHCSFLFSSWI
                     IWALALANLLMVGALEGFADVWGVPYLIAKYALNKTDAAFLISFIFMGMIFGGPLLAF
                     LSQKLGNYFVIAFCGAGLAFLFLILLYTSTLHWWQLGTLFFAIGLLCCYQVIVFAAGS
                     TLVPYEGLGVTIAFLNCINMLGGSFFHTLIGKLMNGCWGGQMSANGLKLYEIESYQWA
                     LSLIPFCALCGSLLIVLIHIKTRAYAAPEFMKKTSLTGV"
     gene            9350..10015
                     /locus_tag="AC06IO_001620"
     CDS             9350..10015
                     /locus_tag="AC06IO_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIO9874807.1"
                     /translation="MEKIFWSNPYQHTLDTKIISINGNEILLEKTIAFSFSGGQESDR
                     AFINGLEVLSSRIDGNLIYYTLPDDHNLAPNDVITMTIDWPRRYRLMRLHFAAELILE
                     LVTRTSMLEKVGAHITESKARIDFKANFNISTIFSQLLDEYNAIIRQDLVIHKGYHDV
                     TNQRRFWKIDGFGEVPCGGTHVHSTSEVGFIRLKRSHPGKAIERIEITLMDDSRYCTS
                     QIS"
     gene            10241..11155
                     /locus_tag="AC06IO_001621"
     CDS             10241..11155
                     /locus_tag="AC06IO_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIO9874808.1"
                     /translation="MELPNHFSTVYVSKPEFIETLSDELGEVSNKIGSLVFSPHQKLD
                     VCFALDVWLEPEIVTFQSISEAAKILRQAGKFWYLHPVTEIRRSRLIEEQLRKCPSLT
                     SHFPVTNDIPPIGVFSLLDRNTLLFSAKRLKKWPDGQCYFIEDKANPPNRAYLKLWEA
                     LFLLNKYPRPGETVLDLGASPGGWTYVMQSVGTQVTSVDKAELDTKIMALPRVSFLKQ
                     SAFALDPEQLTESYDWVLSDVACYPDRAYTLIKKWIASKKARQLIFTIKLQGQTDLNT
                     IKRFQSIPNSKVINLFYNKHEVTFFYPV"
     gene            complement(11228..12181)
                     /gene="epmA"
                     /locus_tag="AC06IO_001622"
     CDS             complement(11228..12181)
                     /gene="epmA"
                     /locus_tag="AC06IO_001622"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HIO9874809.1"
                     /translation="MSDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMAR
                     HGVTDVYLSNIKAIFRGETCYLQTSPEYHMKRLLAAGSGPIFQLARVFRDDELGRWHN
                     PEFTLLEWYQLDINHHVLMDEVDLLLQIILKSQPMIRKTYQQAFEEACGIDPFSTSIE
                     QFTRILAQHGLEGVLSPDEQDRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALA
                     QVQEGLAERFEVYYQGVELANGFHELTDVEAQKKRFMLDQQHRKQKGLTAMEADSYLL
                     EALEHGLPPCSGVALGVDRLLALALKQPSIEKILSFDFSRS"
     gene            complement(12184..12519)
                     /locus_tag="AC06IO_001623"
     CDS             complement(12184..12519)
                     /locus_tag="AC06IO_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HIO9874810.1"
                     /translation="MFGFKKKQSNESIKEGVITALKGVFDPEIPVNIYDLGLIYDIAV
                     NDEGHVHIQMTLTTPGCPVAQTFPGTVEQAVNQVEGVSDCTVELVWEPPWSQDRMTEA
                     ARLELGIFY"
     gene            complement(12591..13040)
                     /gene="sufU"
                     /locus_tag="AC06IO_001624"
     CDS             complement(12591..13040)
                     /gene="sufU"
                     /locus_tag="AC06IO_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIO9874811.1"
                     /translation="MSMELRELYQEIIIDHNRNPRNHHAMEDATTEAKGFNPLCGDKL
                     TVYLKLQGDLIRDVSFVGCGCAISQASASLMTDALKGKSIKEAHELFHRVHRMLTQEE
                     EDSLVSMDKLTVLAGVKAFPARVKCATLAWHTLEAALNKEKEVVKTE"
     gene            complement(13037..14281)
                     /locus_tag="AC06IO_001625"
     CDS             complement(13037..14281)
                     /locus_tag="AC06IO_001625"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIO9874812.1"
                     /translation="MSTNPSLMTSFDVNKIRKDFPVLHQKINEYDLVYFDNAATTQKP
                     KAVIDAIAQFYERDNSNVHRGVHALSVRATEMYEAARAKVKRFINARSPRECIFVRGT
                     TEAINLVAQSLVAPRILPDEEILITHMEHHSNIVPWQMVCKKMGCKLQVAPISLNGEV
                     ILEEFERKLNENTKMVAINYASNSLGTINPVKTMIKMAHEVGAKVLLDGAQATAHLIV
                     DVQDLDCDFYAFSGHKMYGPTGIGVLWGKEELLNSMTPYQGGGEMINSVSFEATEYAA
                     IPHKFEAGTPNIAGAIGLAAAIDYIWSLDLDAIAEYETQLLNYATKAIEAVKGYNIIG
                     TAANKVPIISFVHGKIHAHDIGTILDSEGIAIRSGHHCTMPLMDFYDVAATSRISMSF
                     YNTFKEIDYCMEALQRVKEVFA"
     gene            complement(14271..15557)
                     /gene="sufD"
                     /locus_tag="AC06IO_001626"
     CDS             complement(14271..15557)
                     /gene="sufD"
                     /locus_tag="AC06IO_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIO9874813.1"
                     /translation="MSDILDYYQQQAQSGVSQIPWLAQLQSKALTDLKRRGFPTRYDE
                     EWKYTLVDSLLQQHFVLPDPTVKPALKTVFDDSPVKNLFLIQNGFISQEQEWIKNLPE
                     GVLILPLSLALINYADLVKPYLGKIVEQEHGFHYLNTAMLQCGVFIYIPAGICLEEPI
                     VLKHIQDQTNQAMYLRHLILMEKESQATLIEEYQGEENICYFTNSITEVFLGAGAKLN
                     HYKIQKESKSAYHLGHLSVRQSAASQFSSHSLSLGGKLVRSDISVFMQEEQAQCLMNG
                     IYAPGDGQHIDHHTVVHHLVPNCRSDQDYKGILTGHSRAVFNGKVIVAKNAQHTDAKQ
                     QNKNLLLSANAEIDTKPQLEIFADDVLCSHGATVGQLDEEAIFYLATRGISRDEASHY
                     LIQAFANDNLRLIPNRDLADWMGNLLTQQLGYANEY"
     gene            complement(15554..16306)
                     /gene="sufC"
                     /locus_tag="AC06IO_001627"
     CDS             complement(15554..16306)
                     /gene="sufC"
                     /locus_tag="AC06IO_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIO9874814.1"
                     /translation="MLNIKELNVAVNGQSILKGINLEVKPGEVHAIMGPNGSGKSTLS
                     KVLAGHPSYEITRGDISYLGQDLLPMSPEERARAGIFMSFQYPVEIPGVTNINFLKAS
                     VNAVRKGQGRNTLDAIEFLSFIREKCQLLDMDESFLYRSINEGFSGGEKKRNEILQMA
                     ALEPRLAILDETDSGLDIDALRIISQGVNAMRSPERSIILVTHYQRLLDYIEPDFIHV
                     LANGRIITSGDKSLALELEKKGYSWLEEVEQL"
     gene            complement(16308..17756)
                     /gene="sufB"
                     /locus_tag="AC06IO_001628"
     CDS             complement(16308..17756)
                     /gene="sufB"
                     /locus_tag="AC06IO_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIO9874815.1"
                     /translation="MAKSSEQLNSLLDREYQHGFITDIEVETFPPGLDEDVIRRLSAI
                     KGEPEFLLEWRLKAFRHWLTMQHPEWSSVHYPPIDYQSISYYSAPKSKKDAPKSLDEV
                     DPELLKTYEKLGIPLREQEMLAGVAVDAVFDSVSVATTFKAKLAEKGVIFCPLSEAVH
                     QYPDLVKKYLGSVVPYRDNFYAALNSAVFSDGSFVYVPKGVRCPMELSTYFRINAAST
                     GQFERTLIVADVDSYVSYLEGCTAPMRDENQLHAAVVELIALDGAQIKYSTVQNWYPG
                     DKEGKGGIYNFVTKRGACRGKKSKISWTQIETGSAITWKYPSVILQGDDSVGEFYSVA
                     LTNNFQQADTGTKMIHLGKNTRSTIISKGISAGRAHNAYRGLVRIAPTATNARNYTQC
                     DSMLMGSQCSAHTFPYIEVKNPTAQVEHEATTSKISEEQLFYCQQRGIDTEDAVSMIV
                     NGFCKQVLKELPMEFAVEATKLLGISLEGAVG"
     gene            complement(17749..18210)
                     /locus_tag="AC06IO_001629"
     CDS             complement(17749..18210)
                     /locus_tag="AC06IO_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HIO9874816.1"
                     /translation="MLRVSKLADYGTVVMVYLAKHPQELCNARDIALHTHLTVPTVSK
                     ILKRLTSAGLLTSVRGVSGGYRLQRPAAEISVSQIIFALEEHRGFTECSMQPNDCFLQ
                     GVCTIQGNWRLISHAIETALDSVSLEALAKPTLPTRDVERVRQLASGVNRG"
BASE COUNT         5634 a         3896 c         3398 g         5436 t
ORIGIN      
        1 atgaccgaat taaaaacatt tttaacaaag agattgggat gtttcgtaag tcccggttta
       61 atgagcaata ctggaaatac catttaaagg agtccattta cctatgatta atcaacagat
      121 agaaatattt ttgaaatctc ctgcttttgc cgtagttggc gcctcttcca atagggaaaa
      181 atacggcaac aaagtattgc ggtgctatct tcggaatggc aaaaaagtct atcctgtaaa
      241 tccacatgaa acactgattg aaagcttgcc tgttattcat tcagtaaacg atttacctga
      301 tgatgtacac agtatttcta ttatcacccc tccatccatt actgaagaaa tcgttaagga
      361 agcagtcaaa aaaggaatcc aacatatatg gatgcagcct ggagcagaga gtgactcggc
      421 cgttcaatat ggcttggatc acaagcttaa tgtcatttct gggggacctt gtatattggt
      481 tgtcatgggg tatgaagaga gttaaagcaa aaagctattt tgtagtccaa aatagcttat
      541 ttgattttgt ataatcccag ccgattattt agaactcctg aattgcttta atgattcatt
      601 gaccacactt tcaagagttc tatgttctgc atatttttct cttaaaaagc tggcatcact
      661 ccctttgtga gttatttcat aaatatgctc caaagccata gtactattta gctgattagc
      721 ataaggcttt aatcggcgaa gtgttgttaa aatatcttct cgaagcaaaa tttgctcata
      781 tgttttggga tgcaccagag tgccatccag accaaatcgg catgcttgaa aacgattgta
      841 attgtacaca agataatcat cttcgtgagg cagaggttct ttattttcca ataaataact
      901 gcaaagcgct tgcatataac aagctaaagc agctgctcgc tcaaccgtaa gaggactatc
      961 acacacccgc atttcaattg tcccaaattc aggtttaggc cttaaatccc aataaaaatc
     1021 cttcatactt ttcactatgc cggtatgctc cattttttcg aaataaccga gcgagaattc
     1081 atcccagttt aatacaaagg gagcccttcc acttaatggg aaggcaaaca ccgaattcaa
     1141 tcgagcagag ttatacaagg tatccttact ctgaacgaag ggggatgaag cagacaatgc
     1201 aataaaatga gggatataac gatttaaaga atgaagtaaa aaaagcgctt gattaccgtc
     1261 ctcacaacca atatgtatat gttgcccaaa aatagtaaat tgcttggtta gatagccata
     1321 taattcagac acctctataa acctggtttt attaaaaatt cgctgatcag accacatttg
     1381 aaatggatgg gttcctcctc cacaaatacc aatatttaat cgatctccga ctgtcaccag
     1441 ggcatcgcga atatgaaaca gttgtttcag tagttgctga tattcttcat gcacatctgt
     1501 cgctatttct aacatacttt ctgtgatttc tggcgtaaaa ctgccgggaa aagacgatcg
     1561 tcccaagagc tccaatatat cagggctagc ggcagtcaag tcaaaattac tcaagctgat
     1621 taattgtaat tccagctcaa cccccatagt cagtaaatta gatgttttga atggttgtaa
     1681 aggcatttca aaattccttg attgcagaga tctcgttcgc tttaattaaa gcacgttgaa
     1741 ccattattgg acccaatatt tccaaagtca gagcaaccat agccagcggg gccagtgtat
     1801 ctaccaaatt aataccaata tatcgactct gttcgagaat taaaatgacg aaaacagaga
     1861 taggtgtcat agccaaacca gtcaatacac ctttcttaaa cgaaatccca ctaatatgag
     1921 caaataaact aatcccaata attttagcta cgaaccttac agttataatt gataatccta
     1981 tccatacacc agttactacc ctttgccatt ctatggttgc tgcaataaat acaaacaaaa
     2041 gtactgataa caaatcacct aatgtgccaa atcctggctg tgaaggagaa aagaccattc
     2101 gcctatggcg ggaaaccaag ccaaacgtta atgtagctaa aaccggggac aacttgagat
     2161 tatgggttaa gataaccagc agtatcacag cgcaagcaaa tgccaaggta ttgtcttgac
     2221 aggtgctttt aacggtttta agcaaaatgg gcattaccac tccaaaaata atcccccaaa
     2281 taattgaaat ggctgtaacc gttaagctgg cataagttgc ttcccaaaga tttcctgaag
     2341 ttctgaaaac aaccaagcca atgatgactt tgaataaaaa tactgccagc acacaattga
     2401 tcacagcgag atgtaaaaca cgttctgtca cttggcctga actgcgttgc tcattaatca
     2461 ctctaactac agtggctggt gaagtcgcca tggacaacga agccaataaa aggcaggtcg
     2521 aagtttgtaa cccaaaggat ttcactaaaa tataaaccag aaaaaaagtt aatgtcgctt
     2581 cagtaatact ggatgccgca acccagggat tataacttaa ccagcgtaaa ttaattctat
     2641 agcctgattc aaacaagatt agcccaaaac caatattggc taaaagtaaa atagatggtg
     2701 gttgaataga cggtaataat cctgtttgag ttgatgctag tgaaaatcct acaatcgcat
     2761 agatactgat atgaggcaat ttcgcccatc gatatccaag ttcccctgca aaccaggcta
     2821 tcaataaact gataggccaa gccaagtcgg ttataatttt tccaacatca atcatccata
     2881 cctcctatga aaatccgacc ttactttata acacacatcg atttgttttg cagaattgaa
     2941 gtatttttaa gacaatataa aacaatcagg acttacgaaa aatcccaagg ccgaggcaaa
     3001 aaatgttttt aatgaatgag tttagataaa gtaaatgact gaattaaaaa cttttttaac
     3061 aaagagattg aggtttttcg tgagtactga tagtttcttg tccaattagg aaaatcattg
     3121 ctactaacca cattaacgag aaccaaaaag aaatctcaac aacttcttat tttaataggc
     3181 ttcttattag ggatatcacc agtcatggct ttagaaatta ttactataga aaaaacagaa
     3241 aaccaccata tgctaaaaac tcgggctgaa aatattcaat ttcccttgaa tcgagaggat
     3301 aaagatttaa tagaagcaat gaaaaaaaaa ttatacgcat tgggaggggt tggtttggca
     3361 gcaccacaag tcaattccgc caaacaaatt atcgcagttt acatccctga agaagctcag
     3421 cttttacgtg ataatgcgaa aatctttcca atgcatatcc taatcaatcc aagttacgag
     3481 ccgctgcccc atactcgtat tattagcgac tttgaaggtt gttattctgt atccagtaaa
     3541 gcagggaagg tgccaaggta tgatgaaatt cggctaaaat actatgatga agaagggaat
     3601 gctcaccaac aaatcgaaaa tggcttttac gcccgtgttt tgcaacatga aattgatcat
     3661 ctgaatggtg tgttaatcac cgatcgttta actccagatt gtgtacaagg gactattgaa
     3721 gaaatgatgg ttattcgtcg tgcggaattg cctgtagaaa aacgaaaatt atttgatcaa
     3781 atcatggcaa aaaaacaaaa aaaataacac tgcgtttcca tgttaccttt tatgaattga
     3841 tttcaatgat aaactgccct gggtaatttt tttgcttgtt caacccaggt aacaacttga
     3901 gataatccag gtacatgttg cgccaaatga cattgctcat ttttctcttt taatgagcta
     3961 taaagttgtt caggattgca atgtgctaac tctgggattg agtcaatacc agcctgttcc
     4021 agaagctcag catattcttc attaatgcca tttactcgtg ccagatcagc ttgattggtc
     4081 catttcaaaa tcaatttatg actgattccc gtttgctcgg ctactctaaa acgcccccgg
     4141 cgaccgctac aaactcttaa taaatcgtcc tggctttcaa tccctactcg ctttaacatt
     4201 tcagcatatt ttgggcctac gccttgcaac tcaattaatt tgtgcatttt atttccagtc
     4261 ttctactacg atattacata tataaaatta tagaatataa tgattaatta ttaaccaaaa
     4321 caaatgttga aaaaatttag gcctcacgta ttaaaaaaaa tcaattcatt tggcaaaatg
     4381 aaacaatttc attatgataa ttactttcct ttcagtccct gatgaggtag aaaatgtgca
     4441 agcacaagaa caaaatggtc aggatatttt tattggcttt aggactattt tccagcacca
     4501 ccattaatgc ttggtatggt tcagggataa ccattggtgt tgccagagcg aatgttcact
     4561 attacggaga tgatatccga gtatatgacc cgtggggcta tggatactac gacagaaatt
     4621 attactataa tggagtaaga aacattatcg ttcctaatgt aattgtgccg aatgtagtga
     4681 tcaatgtacc agttcaaagg tattatgtac cctcatgtga ggaaatggaa gtttgtgatc
     4741 cttatggaca atgttggtta gagcaatttt gcagataaat taaaaactca atcaactaat
     4801 gttttgggac tcagcccatt atgggatgag atattagcag gacttacaaa aatcccaaga
     4861 tcaatacaaa aaatgctttt aatgagggag tttagataaa ctgaacaacc gaattaaaaa
     4921 cattttctaa caaagagatt ggggggtatg tcctgattat aattttttaa ggacaaaaaa
     4981 tgtcagaaac tattggttat gccgcccaca aagcgggagc ccctttaaaa cctttcactt
     5041 ttgaacgccg ccctgttggt gcacacgacg tagcaattga tattctattt tgtggtatct
     5101 gtcattccga tttgcatcaa gtcaataatg attgggcagg atcgttctat cctatggttc
     5161 ctggacatga aatcattgga cgagtcaagg aggtaggaca tcaagtccac cggttcaaag
     5221 caggagattt agtgggcgtt ggctgtatgg ttgactcctg ccgtcaatgc acctattgcc
     5281 atcagaatga agaacaatat tgtaaatccg gcgcaaccta cacctataat agtcaagaca
     5341 atcatctggg aggggtgact tacgggggat attccaagca tgttgtcgtc acggaagatt
     5401 ttgtattaaa aattgatgaa tcacttgatc ctgccgccgc cgcaccattg ctctgtgccg
     5461 gaataaccac ttactccccc ttgaaaaggg ctaaagtagg taaaatgagt aaagtaggaa
     5521 tcgttggctt gggtggctta ggacatatgg cagtcaaatt agcccgtgcc atgggggctt
     5581 atgtgactgt atttaccact tctataaaca agataaaagc gggcaaagat ttgggagctc
     5641 atgatgttgt cttgtctacc gacagcaatg tcatgcaaac actggccaat caatatgatt
     5701 ttattcttga tactgtttct gccaaacatg atcttggcca atatttaaac cttcttaaag
     5761 tggatggaca actaacccag gttgggcttc catcagatcc acttgaagtt accatgtttc
     5821 ctgttatttt aaaaagactg agcattaacg gctcattgat tggcggcatt aaagagacac
     5881 aagaagtttt ggatttttgc gccaggcatg ggatcaccgc agacattgaa ttaataaaaa
     5941 tgcatgaagt aaattctgcc tatgaaagac tgctaaaagg agatgtgaaa tatcgatttg
     6001 ttatcgatat gtcaaccttg gattaaatga accaggtata aaaggcttcc agttgacctt
     6061 ttataccacg gagcaaatat tattcctggt ttttgatctt ggggaaaact ttaattttgg
     6121 ttataaaaga atcctgtacc ttttcaatca aggcatcaaa ttcaacaaat tgaattattt
     6181 caccttcatt gggcaatttt ccgtggcgcg tgataataag tccatatagt gtattcagtt
     6241 cttcttcatc tggcgataaa gtaatatcgc aatcaagagc cctttctaga gaataaaggg
     6301 agcaatctcc ttttgtcgtt atagttccgt ccgcattttt tacccaatct tcatgtgttt
     6361 tatgaaactc gtctttaatc cgcccaatca ggatatggag caaattatcc agagttacaa
     6421 atccaagtaa actcgatttg tgtttgtata ccaaagcaaa atgaggcata cctgctcgaa
     6481 atttgcgtag caaatccata gcaggtaatt tatgactgac tttaagtact ggccgtacat
     6541 aaggctttaa tgtattgata tttttctgga gcaacaaatc ggcaaataaa tccttaacat
     6601 ggataacacc aatcatttcc ctagtcccgg aatcatagac aggataacga ctataacgat
     6661 attttaaaat tttatcttta atcgtttcga atggctcatt gatatctatt atgatcattt
     6721 catcttcagg gcgcattacc tcagtgacat ttaactcagc caaatcgaga gtatgctcca
     6781 ggatttcacg ttcatcctct tttaattccg attgcagatg ctgtccgctt aaaattaatt
     6841 tcagttcctc acttgaataa aaaccttcgt attcattttt tatatccatg ccggacagtt
     6901 tgagaaaaaa attggaacat gtatttaaca accaaatggc aggatacatt atccagtaaa
     6961 accaatacaa cgggactgca gtccatattg aaatcaattc agattgccga atggcaatgg
     7021 acttgggcat gagctcaccg attacaatat gtagaaaaga aataagaaaa aatgcaataa
     7081 aaaaagaaga tatagtaaca atatgaaccg atgaaatacc caaagcaaca aataatggcc
     7141 ccaataactc cgcaaaagca ggctctccaa tccaacccaa tcctaacgaa gcaagagtaa
     7201 tgcctaactg acatgctgat aaatacgcat cgagattttg atggacttgc tcaagaatgc
     7261 gacctcttag cccgtagttt actttgatgg cttgaattct cgtagccctg agtttaacca
     7321 tcccaaattc ggccgataca aaaaatgcat tgagaagcac cagcaggaat gctataaaaa
     7381 ttaacagaat gttatacatt aaaaatagtc cggtttatga tttaatttca cagaacttac
     7441 gaaaaatccc aatctcttcg ttaaaaatat ttgtaattcg atcatttggt ttgtataaaa
     7501 tccctcatta aaaatatttt ttaccttggc cttgtggttt ttcgtaagtc ctgttttcta
     7561 ttcagtcgta ccttcactaa aaacattcac tctctacaaa tgtgatcgaa tcttttatgt
     7621 taatagaata tcgcagtcct ggcgagttta aaatgactaa gccagaaccc aaagatacaa
     7681 taattatcat ccggtttaat gacaaattat ttaatcaaag caaccgtata gaaatgacac
     7741 ccaataccag taaatagcgg cccctattga caaatagacg cacaatttgt taatttgact
     7801 cattaaaaga caaaattaac tcaacctgaa aagattatga atcgtccaaa cagcaaaaca
     7861 tgccatgact tttgtgcaaa ttcttatgcc aatgttttgc tttgcgacgc atcccaatcc
     7921 atagataacc tttcaatcat tattattatc ttgccctctt tcccctagag cggtcagctg
     7981 ttgctaattc tctgcctctc tagggaggct tccaaagagt tagcaccaac tgatttcaac
     8041 tggagggcgt catgaatcaa aattgtttca aaccaaagaa taatttcgtc tcattggtga
     8101 tgtgggcatt acctttatct ttttttactt ttcaattctt attacgtctt tggcctggat
     8161 taaccatgca agacattatg gcgcaatttt caattgatgc cgcaagcttt ggccttatcg
     8221 ctgcattcta ttattatgga tacgcaggaa tgcaaatccc cgtagcctta ctgctggata
     8281 aatttggagc ccgcttcgtt gtatttgcct ttgcaatgtt gtgtggtatt ggaactttag
     8341 ttttcataag taccaataat ttctacttgg ctctgtcagg caggtttttg ataggtgtag
     8401 gctccgcagc tggttttctt ggcgtatcca aagtcgtatc agagtggttc agccattcac
     8461 agtactccag aatgatcggt ttctccttta gcgtaggctt gctgggggcg atctatggag
     8521 gaaagccgct tgcccacctg acacagaatt ttagtgggca acaagttggt ttggctttgg
     8581 cggttttctc tatcctctta ggatgttgcg cgcttttgat cttgcgctct cccccaaata
     8641 atactcgctc agtaacaaaa acaatgaatt tgtctcactg ttccttttta ttttcatcgt
     8701 ggattatctg ggctcttgct cttgctaatt tattaatggt cggtgccctg gagggattcg
     8761 cggacgtatg gggagtacct tatcttatcg ccaaatacgc gctgaacaaa actgatgctg
     8821 cctttctcat ttcgtttatc tttatgggaa tgatatttgg cggtccttta ctcgctttcc
     8881 tcagtcaaaa attaggaaac tactttgtaa tagccttctg tggtgctggt ttggccttct
     8941 tgttcctaat ccttctttac acctctaccc ttcactggtg gcagttgggc actctcttct
     9001 ttgcaatcgg cctgctgtgt tgctatcaag ttattgtatt cgccgcaggt tctactttgg
     9061 ttccttatga aggtttaggc gtcaccatcg ccttcttgaa ttgcatcaat atgttaggcg
     9121 ggtctttttt tcatactttg attggtaaat taatgaatgg ctgttggggc gggcaaatga
     9181 gcgctaatgg ccttaaatta tatgaaatcg aatcgtatca atgggctttg tcactcattc
     9241 cattctgtgc cttatgcggc agtttgctta ttgtattgat tcatatcaaa acaagagctt
     9301 atgcagctcc agagtttatg aaaaagactt cattgacagg agtgtagcta tggaaaaaat
     9361 tttttggtct aatccctacc agcatacact cgataccaag ataatctcaa ttaatggcaa
     9421 cgagattcta ttggaaaaaa ccatcgcgtt ttcattttca ggaggacagg aaagcgatag
     9481 agcattcatc aatggccttg aagtactcag ttcacgaata gatggaaatc ttatttatta
     9541 taccctgcct gatgaccata atcttgcgcc caatgatgta atcaccatga ctatagattg
     9601 gcctcgaaga taccggctga tgcgtcttca ttttgcagca gaactgattc tggaacttgt
     9661 aaccagaacc agtatgcttg aaaaagtagg agctcacatc acggaatcca aagctcgaat
     9721 agattttaag gcgaatttta atatctctac tatttttagc cagctgcttg atgaatataa
     9781 tgccatcatt agacaagatc ttgttatcca taagggatac catgatgtga caaatcaacg
     9841 tcggttttgg aaaattgacg gttttggtga agtaccttgt gggggaacac atgttcattc
     9901 cacttcagaa gtgggtttta ttcgtctcaa acgctcgcat cctggcaaag ctattgaacg
     9961 cattgaaata accctgatgg atgatagtag atactgcact tcacagataa gctgattgtt
    10021 ttgctttggg gcaaggtgtg aattttatgg ggcacggagt ttatatacat tgaatgagcg
    10081 ttgctgaaac attcggatcg tgacagtaaa ataaataaag cgggttattc aggaagccag
    10141 gtcaaacgct aatcgagaca aaaatataga aaaagaatac aaaatcgcct aaattacagc
    10201 atttgacttt gtcgccatta acctgcgagc gttatttatc atggaactgc ccaatcattt
    10261 ctctactgtc tatgtgtcaa aaccagaatt tatagaaaca ctttctgatg agttaggcga
    10321 ggtatccaat aaaataggct ctctcgtttt ttctcctcat cagaagcttg atgtttgttt
    10381 tgcactggat gtatggcttg aacctgagat agtaaccttt caatcgattt cagaagcagc
    10441 aaaaatattg aggcaagccg gaaaattctg gtatttgcac cctgttactg aaattcgccg
    10501 atcgcgcttg attgaggaac aacttcgcaa gtgtccatca ctaacgtccc attttccagt
    10561 tacaaatgac attccaccca taggtgtatt tagtcttctt gatagaaaca ctctactctt
    10621 tagcgccaaa cgactaaaaa aatggccgga tggacaatgt tattttattg aagacaaagc
    10681 caatccccca aaccgggctt atttaaaatt atgggaagcc ttgttcttgt taaataaata
    10741 ccctcgcccc ggtgaaacag ttttagattt gggcgcatcc ccgggaggtt ggacatatgt
    10801 gatgcaatca gtcggaaccc aagtcacttc ggtcgacaaa gcagaactgg atacaaaaat
    10861 catggcgctt cccagagtca gttttttaaa acaaagcgcc tttgccctag atcccgaaca
    10921 acttacagag tcttatgact gggtactttc cgatgttgcc tgctatcctg acagagccta
    10981 caccctcatc aaaaaatgga tagcatcaaa aaaagcaaga cagttgattt ttaccatcaa
    11041 actacaggga caaactgact taaacacaat caaacgattt caatccatac ctaattctaa
    11101 agtaatcaat ctgttttata acaaacatga agtcacgttt ttttatccag tataaacata
    11161 accaggattt agtgattatg tctgcaaaaa tatcataatg acaagctaat acttaatttt
    11221 ctatgactta agaccttgaa aaatcaaatg agagaatttt ttctatgctt ggttgtttta
    11281 aggcgagcgc caacaatcga tcaaccccaa gagccacccc gctgcaaggt ggtaacccat
    11341 gttccagggc ttccaaaaga tatgaatcgg cttccattgc cgtcaatccc ttttgctttc
    11401 tatgttgctg atccagcata aaacgctttt tttgcgcttc gacatcagtc aactcatgaa
    11461 aaccattagc caattcaact ccctgatagt acacctcaaa acgctcagcc aagccctctt
    11521 gtacttgtgc cagggcagcc tgggaggcag ggaagtgata cacagcaacg ggggttgatt
    11581 cttctgccaa aaaaggctcc acgacatggc tcattaataa aaacagatat tgatccctgt
    11641 cttgttcgtc aggagataag acaccttcta aaccatgctg tgctaatatc ctggtgaatt
    11701 gttctatgga tgtagaaaaa gggtcaatac cgcaagcctc ttcaaaggcc tgttgatagg
    11761 tttttctgat cataggctgg gatttaagaa taatttgtaa taataaatcc acttcatcca
    11821 tcaaaacatg gtgattgata tctaattgat accattcaag taaagtaaat tccggattat
    11881 gccaacgccc taactcgtca tcgcgaaaaa ctcgcgctaa ttgaaaaata ggcccactgc
    11941 cagcagctaa cagccgtttc atatggtatt caggggaagt ttgtaaatag catgtttcac
    12001 ctctgaaaat cgctttgata ttgctcaggt aaacatctgt aaccccatgc cgcgccatca
    12061 caggggtttc cacttccaga tattgacgtt cagtaaaaaa ttgacggatt ttacctatta
    12121 gctcggctcg cgcacgtaat aattgaattg aagcagaggg caaccaggag ttatcagaca
    12181 tgatcaataa aatattccca actccaagcg ggcagcctct gtcatgcggt cctgagacca
    12241 gggcggctcc cacaccaact caacagtgca atcgctgacc ccttcaacct gattgactgc
    12301 ctgctccacc gtaccgggaa aggtttgcgc cacagggcac cccggggtcg ttagcgtcat
    12361 ctgaatatga acatggcctt cgtcattaac ggcaatgtca taaatcaagc ccagatcata
    12421 aatattgacc ggaatttcgg gatcaaaaac tcccttcaac gcggtaataa cgccttcttt
    12481 tattgattcg ttactctgtt ttttcttaaa accaaacatg agctgcctct ggtctatttt
    12541 gcaatataca gacaaactct ttcacgagct ctgccactat tgccataaca ttattctgtt
    12601 ttcaccactt ctttttcttt gtttaaagcc gcttccaaag tatgccacgc caaagtggcg
    12661 cattttacac gcgcagggaa tgctttaact ccggctagaa ctgttaattt atccatggaa
    12721 accaaagaat cttcttcctc ttgtgtcagc attcgatgaa cacgatgaaa tagctcatga
    12781 gcttctttaa ttgatttacc ctttaacgca tcagtcatta acgatgcaga agcttgtgag
    12841 atagcacagc cacatccaac aaaactgaca tcacgaatca aatccccctg caacttgagg
    12901 taaacagtca gtttatctcc gcataaaggg ttaaaccctt tcgcctcggt agtcgcatcc
    12961 tccatcgcat ggtggttacg aggattacga ttgtgatcga taatgatttc ctggtaaagt
    13021 tcccgcaact ccatactcat gcgaatacct ctttaactcg ttgtaaggct tccatacaat
    13081 aatcaatttc tttaaacgta ttataaaagg acatggaaat acgagaggtc gcagcgacat
    13141 cgtagaaatc catcaaaggc atagtgcagt gatgtccgct gcgtatcgct ataccttcac
    13201 tatccagaat agttccaatg tcatgggcat gaatttttcc gtggacaaaa gagataatgg
    13261 gtactttgtt ggcagccgtt ccaataatat tgtacccttt tactgcttca atagccttgg
    13321 tagcatagtt taacaattgt gtttcgtatt cagctatcgc atccaaatcc aatgaccaaa
    13381 tataatcaat tgcagcagcc aaaccaatag cccctgcaat attcggggtg cctgcttcaa
    13441 atttatgggg gatagcagca tattctgttg cctcaaaact cacggagttg atcatttcac
    13501 ccccgccctg ataaggagtc atgctattta ataattcttc ttttccccat aaaacaccta
    13561 ttccagtggg accatacatt ttatgtccgg aaaaagcata gaaatcgcaa tccagatctt
    13621 gaacgtccac aatcaggtga gccgttgcct gtgccccatc cagtaacact ttagccccta
    13681 cttcatgtgc catcttaatc attgttttaa caggattaat agttcctaaa gaattggagg
    13741 cgtaattaat cgcaaccatt ttagtgtttt catttaactt tctttcaaac tcttcaagta
    13801 ttacctcgcc atttaaagaa ataggggcaa cttgcagctt gcaacccatt ttcttacaga
    13861 ccatttgcca aggaactata ttggaatgat gttccatatg ggttatcaaa atttcttcat
    13921 caggtaatat gcgaggggct acgagactct gggctactaa gttaatggct tctgtcgtac
    13981 cgcgcacaaa aatacattct ctcggtgatc tggcattaat aaatcgtttt actttagctc
    14041 ttgctgcctc atacatttcc gtagccctga cactcaaggc atgaactcca cgatgtacgt
    14101 tggaattatc cctttcataa aattgagcaa tagcatcaat cacagccttg ggtttttgag
    14161 tagtagccgc attatcaaaa tacaccaaat catactcatt gattttctga tgcaatacag
    14221 ggaaatcttt cctgattttg ttgacatcaa aggatgtcat taatgaagga ttagtactca
    14281 tttgcgtacc ccaactgttg agttaataaa ttacccatcc aatcagccaa atcacgattt
    14341 ggaataaggc gcaaattatc attggcaaat gcctggatca aataatgaga agcttcatcc
    14401 cggctaatac cacgtgttgc caggtaaaat attgcctctt catccagttg cccaaccgta
    14461 gcgccatgtg aacataacac gtcatcggcg aaaatctcta attgcggttt ggtatcaatt
    14521 tcagcatttg cggagagaag taaattttta ttttgttgct tggcatcagt atgttgcgca
    14581 ttcttggcta caataacctt gccattaaat accgctctgg aatgtcctgt taaaattcct
    14641 ttgtaatctt gatcgcttcg gcagttaggc actaaatgat gaactacagt atgatgatct
    14701 atgtgttgcc catcccccgg cgcataaatt ccattcataa ggcattgagc ctgttcttcc
    14761 tgcatgaaca cgctaatatc actgcgtacc aacttcccac ccaaacttaa agagtggctg
    14821 gaaaattgac tggcggcaga ttgcctaact gataaatgac ctaaatggta agctgattta
    14881 ctttcttttt gaattttata atgattaagt tttgctcctg ctccaagaaa cacttcagta
    14941 atactgttag tgaaatagca aatgttttct tcgccttgat attcttcaat cagagtagcc
    15001 tgagattctt tttccataag gatcagatgc cttaaataca tagcctggtt cgtttgatcc
    15061 tggatatgct ttaaaacaat gggttcttcc aaacaaattc ccgcaggaat ataaataaag
    15121 actccgcatt ggagcatagc tgtatttaga taatgaaacc catgctcctg ctctacaatt
    15181 tttccaaggt aaggcttaac caaatcagca taatttatta aagcaagaga taaaggcaga
    15241 attaacacgc cttcaggcag attcttaatc cattcttgct cttgggaaat aaatccattc
    15301 tgtattaaaa acaggttttt gactggtgaa tcgtcaaaga cggttttaag agcaggcttt
    15361 actgtaggat caggtagtac aaaatgttgc tgtaatagtg agtcaacgag agtgtacttc
    15421 cattcctcat catatcgggt gggaaaacca cggcgcttca aatcagttaa tgctttagac
    15481 tgcaactgcg caagccaggg tatttgcgat acgcctgatt gggcttgctg ttgataataa
    15541 tccaaaatat cactcataac tgctccactt cttcaagcca gctataacct tttttctcca
    15601 gttctagcgc caaagattta tcccctgacg taataatacg accatttgcc aagacatgaa
    15661 taaaatcagg ctcaatataa tccagtaagc gctgataatg ggtcactaaa ataatagaac
    15721 gctcgggaga gcgcatcgca ttaactcctt gagaaataat tcgtaatgcg tcgatatcca
    15781 ggccagagtc ggtttcatca agaatagcta gcctgggctc caaagcagcc atttgcaata
    15841 tttcattacg tttcttttcc ccacctgaaa atccctcatt aatacttcgg tataaaaagc
    15901 tttcatccat atccagaagc tgacattttt cccgaataaa acttaaaaac tctatggcat
    15961 cgagggtatt tcttccttgt cctttgcgaa ccgcattcac tgaggcttta agaaaattga
    16021 tattggttac accaggaatt tctaccggat attgaaaaga cataaaaata ccggcccgtg
    16081 ctctctcttc cggggacata ggtaacaaat cttgtcccaa ataggatata tcaccacgag
    16141 ttatttcata agaaggatgc ccagccaaca ccttggagag agtacttttt cctgaaccat
    16201 taggccccat aatcgcatga acctctcctg gtttaacctc aagattaata ccttttaaaa
    16261 tagactgtcc attgacagca acattgagtt ctttaatgtt taacatatta gccgactgcc
    16321 ccttctaagc ttatacctaa taatttagtg gcttccaccg caaattccat aggtaattct
    16381 tttaaaactt gcttacaaaa tccattcacg atcatagaaa cggcatcttc tgtatcgata
    16441 ccgcgttgct ggcaataaaa caattgctct tcactaattt ttgaagtagt ggcctcatgc
    16501 tctacttgtg ccgttggatt tttgacttct atataaggaa aagtatgtgc tgaacattga
    16561 ctacccatta acatggagtc gcattgggta taattacgcg cgttagtggc tgttggtgcg
    16621 atacgtacca atcctcgata agcattatgc gcacgcccgg cactgatccc tttcgaaata
    16681 attgttgatc gagtattttt ccctaaatga atcattttcg tacctgtgtc ggcttgctga
    16741 aaattattgg ttaaggcaac agaataaaac tcgccaacag aatcatcacc ttgcaaaatc
    16801 acactggggt atttccacgt aatggctgaa cctgtttcaa tttgtgtcca ggaaattttc
    16861 gatttcttac cccgacaagc tcctcgtttg gtcacaaaat tgtatatccc ccctttacct
    16921 tctttatcac caggatacca gttttgtacc gttgaatatt ttatttgagc cccatctaac
    16981 gcaatcaatt caacgactgc ggcatgcaac tgattttcat ctcgcattgg ggcagtacac
    17041 ccttccaggt aggacacata gctgtcaaca tcagcaacaa taagtgttct ctcaaattgc
    17101 ccggtagaag cagcattgat cctgaaatag gtcgatagct ccatcggaca tcggacacct
    17161 ttagggacat aaacaaatga tccatcacta aatacagcag agttcaaagc agcataaaaa
    17221 ttatccctgt aaggaacaac tgaccccaga tatttcttaa ccaagtccgg atattgatgt
    17281 accgcttccg ataatggaca aaaaattact cctttttcag ctaatttcgc tttaaaagta
    17341 gtcgcaactg aaacactgtc aaacaccgca tcaacggcaa cacctgccag catttcctgt
    17401 tcgcgtaaag ggatacctaa tttctcataa gtttttaaca actccggatc aacttcatca
    17461 agacttttgg gagcgtcttt tttactttta ggcgcggaat aataggaaat ggactgataa
    17521 tcaattggag gatagtgcac actcgaccat tcggggtgct gcatagtgag ccagtgccga
    17581 aatgctttca accgccactc aagcaaaaat tcaggttcgc cttttattgc ggacaaacga
    17641 cgaatgacat cctcatccaa tccgggagga aaagtttcaa cttctatgtc agttataaaa
    17701 ccatgttgat attctctatc gagcagagaa tttagttgct cactgctttt agccacgatt
    17761 tactccactt gctaattgcc gaactcgctc aacatccctt gttggaagag ttggttttgc
    17821 caatgcctcc aaactgacac tatcaagagc agtttcgatc gcatgactta ttaatcgcca
    17881 atttccttga atggtacata ctccctgtaa aaaacaatca ttaggttgca tactgcattc
    17941 tgtgaaaccc ctgtgctctt ccaaagcaaa tatgatttga gacaccgaaa tttcagccgc
    18001 tggtctttgc agtctatatc ctccggatac acctcgtacc gaagttaaca aacccgcact
    18061 agtcaggcgt tttagtattt tactaacagt aggtaccgtt aaatgcgtat gtaaagcgat
    18121 atctcgcgcg ttgcacaatt cctgcggatg cttggctaga taaaccatta caactgttcc
    18181 ataatcggcc aacttactga cgcgcagcat aacacccccc actcgaataa tctagtacca
    18241 aataagtctt attttcagat agaggaaaag aatatcttat taagaacaag gattagacaa
    18301 ttgcttactg atccaatatc acctgatcct ctaagtgagc ttttctggca caatcatcca
    18361 aagc
//
LOCUS       DBNCWK010000055        18176 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.055, whole genome shotgun sequence.
ACCESSION   DBNCWK010000055 DBNCWK010000000
VERSION     DBNCWK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18176
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.055"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..908
                     /locus_tag="AC06IO_001630"
     CDS             <1..908
                     /locus_tag="AC06IO_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9874817.1"
                     /translation="TRICIKNFFVGLWFSTLIIILASYLLAKKSMRPIKIFTKELASI
                     NASSLEKRLPYTGNPKELEELASTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLR
                     TATEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNLRTLSAN
                     HLISSIIEYYYSIAQEKGIEIQVFGEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRIL
                     IIIEKRTDDSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENAGLGLGLAIAKSI
                     MEHHKGQLAVDSKIGLGTTVILTLP"
     gene            complement(1074..3068)
                     /locus_tag="AC06IO_001631"
     CDS             complement(1074..3068)
                     /locus_tag="AC06IO_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIO9874818.1"
                     /translation="MSDTPFIAANKKVAELTFRVIILAIILTVLLAMSNAYLALKLGI
                     LTSASIPAAIISMGILRFFKNSTILENNAVQTAASAGEAVAGGIVYTIPALIIIGFWN
                     HFDYVTNFFIAVCGGILGVLFSIPLRRILVNDQTLKFPEGRAIAEVLKSSADRVGIKD
                     IFLGGLVGGLLELLQIGFKVIASSWNYWFVVKRSLFGLGAGFSATMIGAGYLVGHDMA
                     ISIFLGAVISWLIALPIASQFYPDFLNQYPAEQAATLLWNSEMRYLGIGAMLFAGIWT
                     FIKLVKPLTRSVKTSFRTYISKSKFREQYPRTEKDIPMPFILMGIGLMAGILFLFFQI
                     IFPLGQVGLDNDFSPTIVFIGVIYVLVVGFLFSVITAYFSGMVGVTASPGSSVVISGM
                     LFAAWLLVSAINHLLPLPLTSHQIQAAEAITIIIGAVVTGIAAIANDNTQDLKVGQLV
                     GATPWKQEVMLLLGVVISALVIPPVMQILFNVYGIAGVMPHAGMDVTQSLPAPTAALM
                     AAITEAVFRNSLPWNMMLAGSCIILLAIFLNRILNIKRFLNLSILGIAIGMYLPLSSS
                     VPLFIGGMIALFVQKQLGRVKMAPEESMIRKQKGTLIACGLVAGAAIMDVLLAIPFSI
                     FHSPDALQLVRPSWKHYGVYLGVVSTILLAGWINRRVCRS"
     gene            complement(3068..3763)
                     /locus_tag="AC06IO_001632"
     CDS             complement(3068..3763)
                     /locus_tag="AC06IO_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HIO9874819.1"
                     /translation="MPIQMNLINKIGLMFGLLFYSFLASAITTEVIKKETAEYLLDIK
                     YPQGFQSNGVNQAVKEFITNQQKSFMNELSEDADTPADAPGKTGLNITYAIPYQSTNA
                     LSVRFNISIYHRGAAHPSNAVKVLNFIKNQPVQLSDLFVPGSDYLQPIADLAKKEITA
                     KKISDENWIKEGTKPTQENYSIWHFTKKGIAIVFNTYQVAAYVYGEQTVDIPLSLISA
                     MVKPEISKAVWGN"
     gene            complement(3790..5001)
                     /gene="hutI"
                     /locus_tag="AC06IO_001633"
     CDS             complement(3790..5001)
                     /gene="hutI"
                     /locus_tag="AC06IO_001633"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIO9874820.1"
                     /translation="MFACDELLLNASTIDATGLQLSNQAIVIRKGRIEWCGSEDQLPV
                     HFQESAKSRKDCHGQLITPGLIDCHTHLVYAGHRAAEFRLKLQGVSYADIAKSGGGIL
                     STVQMTRDASEEELIDQSLPRLLALKNEGVTTVEIKSGYGLDLQNELKMLRVARQLGD
                     TAGVRVKTTFLGAHAVGPEFKGNSQAYVDFLCNEMLPAAKNMGLVDAVDVFCESIAFS
                     IRQAEQIFQAAKNLNLPIKCHAEQLSNMGASSLAARYGALSCDHLEFLDENGALNMVK
                     ANTVAVLLPGAFYFLKEKQKPPVDLLRQVGVGMAIATDSNPGSSPTTSLLLMMSMACQ
                     FFSMSIPEVLSAVTYQASRALGMEKDIGSIEAGKIADLVLWSIKDSAALCYYFAYPLP
                     HQTMVAGEWVS"
     gene            complement(5001..5810)
                     /locus_tag="AC06IO_001634"
     CDS             complement(5001..5810)
                     /locus_tag="AC06IO_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9874821.1"
                     /translation="MSGIISPIVFYIDEEIVYLITGGGSGIGRALAFALAKRNKQVFI
                     VGRREKLLQETASLYANIHYLCADVSTPQGRELIKASLSATPQIDGLINNAGTLEPTL
                     LLKDMSFAEWHQALNTNIDAALFLPQLLYDKLSGGRVLNVGSAAAYFPIKGWGAYCVS
                     KAALAMLTRCWQIECDSLAFASVMPGIIDTHMQAIARNGINLDPEQVNFYRRLKDHNR
                     LISPETVAEFFIWLLLDIDNETYVSKEWDIYDTTHHSAWLKPPHQVLHWEF"
     gene            6097..7056
                     /gene="hutG"
                     /locus_tag="AC06IO_001635"
     CDS             6097..7056
                     /gene="hutG"
                     /locus_tag="AC06IO_001635"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HIO9874822.1"
                     /translation="MFDDLSNYRPANPALWQGRKDTANQERFFQKITFIDNQNELMTK
                     DKKTIFLGFASDAGIKRNLGRTGAKLGPDQIKTQLAKLPCHNNKHYVDLGNVICENDE
                     LELSQSQFAQIVHFCHENGHQICAFGGGHEIAWAHYQGLSSLYPKLGVINFDAHFDLR
                     PYKKGEFGNSGTPFSQIAAYCEEKKMPFHYCCIGVQKFGNTPSLFEKAKKLNVSYLSA
                     EDLYEQSQAWQIAFLDDFILNLDHIYLTICLDVLAECYAPGVSAPQALGLSPWQIMPL
                     LKYLIQSGKVVSLDIAELSPPLDSELKTARLAALIIAELLDTN"
     gene            7069..8043
                     /locus_tag="AC06IO_001636"
     CDS             7069..8043
                     /locus_tag="AC06IO_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HIO9874823.1"
                     /translation="MKKTVLWGALFTLITTQANSEATQQAISTQNSASTGTNVVSEPT
                     NQVPATNQNTQQNQLNQPVQSNGQQPTQAIQNNQQNQPSQTGEQQSNQNTQNNQQNQP
                     AQQQTTQQPAQQQVPSQQTAPVINCDYKISAETKAIEQSLVLTWSEKAIIQSFDFDPA
                     TVDAQLQKLQACFTDQGWLSFNSALQKSGNLDAIKSQKLHVSSQIDGQPQVTEAIENQ
                     WKIIIPLQVVYQNDKEKVTQLLNVNVTVGRKITGDLGINQMIAVPRTATNTSQTNSPS
                     NTNPADSNTSTSQTPGDTTNNATQTNTQTQTGTPTNSPPSGSTTNTQN"
     gene            8100..9272
                     /locus_tag="AC06IO_001637"
     CDS             8100..9272
                     /locus_tag="AC06IO_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIO9874824.1"
                     /translation="MRLIFTGVLCLLTLNAQANVIQYFAGISYNNPADLFKVKNGILL
                     IGGTGSYADLQFKGSALNFNTFQYDSGVNHSRTYIVWPYGRVAKRLNDKTVVAVDLTE
                     PFNSNLDWGNDAFTRYAATQNYLTDVDLSPKISYAISKKLQIGGGINFNVLLKNEVNW
                     AFPTGQSTYANLINRSSSFGVGYNLGINYAVNDTNFLGITYYSRIRQNTSGTSYLGLA
                     ANPDFQFGFYMPATTVASYVHIFNPKWLINLQVFQSEWNANQKVRLYNTAAPPPFTNF
                     IFDMHFDTSYAYLAAIRKQVSDKLGIALAGMIDDGPEEDGLRTIVFPSDTQYFLGLIG
                     DYRFTEHASMELILGHVYSNPSIQNKAKVNNVPVPFTTGRVTINANVLDLKVKIEG"
     gene            complement(9312..9737)
                     /locus_tag="AC06IO_001638"
     CDS             complement(9312..9737)
                     /locus_tag="AC06IO_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIO9874825.1"
                     /translation="MYNIMSFLCGLLFGAGLTISNMINPNKVLNFLDINGFWDPSLLI
                     VMAVAVTVTFLGYKLINKFDKPILCNQFYLPEKKSIEKKLILGSIIFGIGWGVAGYCP
                     GPSISALTTFNSDPVYFVIGLIAGSWTYYWLFKKKTIQQ"
     gene            complement(9737..10174)
                     /locus_tag="AC06IO_001639"
     CDS             complement(9737..10174)
                     /locus_tag="AC06IO_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIO9874826.1"
                     /translation="MDYITSFTPYLSAIGGLLIGLSAVLFLWLNGRIAGISGMIHGLC
                     PPEKPFPVWRIAFLLGLAAAGLIYFISPVIQFPLRTHYPVFLLLIGGFLVGFGTRMGQ
                     GCTSGHGVCGLARFSKRSFIATLLFILSAMITVYLIRHVGGLY"
     gene            complement(10284..10895)
                     /locus_tag="AC06IO_001640"
     CDS             complement(10284..10895)
                     /locus_tag="AC06IO_001640"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIO9874827.1"
                     /translation="MKKIYWAVILMCLGLTSCVEFDESTLITDDAGYVHRTVHYLRDK
                     RGRNYFPMQIKAPGERLFVFDPKASAWAAYDEEGHRVMTGAGSGGIDVCEENPNQSCR
                     TITGTFRIYNKRGIDCRSGEYPVDTKGGAKMPYCMYFYRGFTIHAAYEVPEHPSSHGC
                     VRVFPSAAKWLNEEFLRVGTKVIVLAYPDQNGVKTMSSLEKVN"
     gene            complement(10937..12610)
                     /gene="ettA"
                     /locus_tag="AC06IO_001641"
     CDS             complement(10937..12610)
                     /gene="ettA"
                     /locus_tag="AC06IO_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIO9874828.1"
                     /translation="MAQYIFTMNRVSKIVENQRFILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGVDTQYEGEARPQPGIKIGYLAQEPELDLNKTVREIVEEGVAEIKEKLS
                     RFDAISMRFAEPMSDDEMNALLTEQGELQNEIEACGGWDLERKLDVAADALRLPDWDA
                     IIEKLSGGERRRVALCRLLLSSPDMLLLDEPTNHLDAESVAWLEHYLEEFTGTVVAIT
                     HDRYFLDNAAEWILELDRGEGIPYKGNYSSWLEQKEARLSMEKKQEDALQRAIKAELE
                     WVRTSPKGRHAKNKARLARFEEMNSKEFQKRNETNEIYIPPGERLGDLVLEGEKICKS
                     YGDRILIDNFDFKLPKGGILGIIGPNGAGKSTFLKMLTGQEEPDKGVIRIGETVKLAY
                     VDQMRDNLNPNNSVWQEISDGHDIMQIGNFQMPSRAYVGRFNFKGTDQQKKISQLSGG
                     ERNRVHLAKLLKSGGNVLLLDEPSNDLDVETLRALEEAILNFPGCAIVISHDRWFLDR
                     ICTHLMAFEGDSQITFFEGNYSEYEADRKRRLGDEANRPSRIKYRKLSD"
     gene            complement(12653..13675)
                     /gene="tdh"
                     /locus_tag="AC06IO_001642"
     CDS             complement(12653..13675)
                     /gene="tdh"
                     /locus_tag="AC06IO_001642"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HIO9874829.1"
                     /translation="MKSLVKAKKEPGIWMQDIPVPEYGVNDVLIKIKRTAICGTDIHI
                     YSWDEWAQATIPVPMTVGHEFYGEIVEVGKEVQGLKVGQRVSGEGHITCGFCRNCRAG
                     KRHLCRNTLGVGVNRPGCFAEYLALPATNVIALPDNITEEQAAILDPFGNAAHCALAF
                     DVVGEDVLITGAGPIGIMAAAIVRHIGARHVVITDVNDHRLELARQMGVSRAVNVKYQ
                     KLSDVANELGMLEGFDVGLEMSGNPMALNDMMKAMNHGGHVALLGIPPQETPIDWNQV
                     IFKGLVIKGIYGREMFETWYKMIAMLQSGLNISPVITHNFPVDEYQHAFQIMASGQSG
                     KVILNW"
     gene            complement(13761..14945)
                     /locus_tag="AC06IO_001643"
     CDS             complement(13761..14945)
                     /locus_tag="AC06IO_001643"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIO9874830.1"
                     /translation="MLERFINYLQNELETLKADGLYKSERVISSQQQADVKVNEREVI
                     NLCANNYLGLANDPELIAEGQAALAKYGYGMASVRFICGTQTPHKQLEQKISHFLSKE
                     DTILYSSCFDANTGLFETLLTEEDAIISDALNHASIIDGVRLCKAARYRYANNDMKAL
                     EEQLIAAKNARFRLIATDGVFSMDGILANLPAICELADKYDAMVMVDDSHAVGFMGKT
                     GRGTPEHFGVSDRIDIVTGTLGKALGGASGGYTASNQVVIDWLRQRSRPYLFSNTLAP
                     VIAHTSCVVLDKLSTNNSLAEKLKRNSHYFREGMTQLGFELIPGEHPIIPVMLGDASL
                     AGRIANRLLELGVYVVGFSYPVVPKGLARIRTQMSAALELHHLDKALKAFETVGKEFS
                     VI"
     gene            15126..15776
                     /locus_tag="AC06IO_001644"
     CDS             15126..15776
                     /locus_tag="AC06IO_001644"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIO9874831.1"
                     /translation="MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPELFSNF
                     AYSDMQIPLAHGQRMLTPLEEGTIIQALDLKGHETVLEVGTGTGFMTALLSKLCKKVI
                     SIDYYSDFTANAKRKLEENNCNNVELITGDACRGWLESAPYDVMVFTGAMEKLTDTHK
                     LQILPGGKLFAILGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF"
     gene            15788..17155
                     /locus_tag="AC06IO_001645"
     CDS             15788..17155
                     /locus_tag="AC06IO_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIO9874832.1"
                     /translation="MRKSLFCWILTLGVSTHVFATDLMDIYQQALENDTIFKEAYDTY
                     MSSTEAIPQARAALYPQVGLGSQAGRNYQDAVAGAFSANQYYGSYLWQVNASQALFNY
                     QAWAKVAQAKASVKAAQATFNDAAQNLILRTAKAYFDVLFAKDTLDFAEAKKRANKRQ
                     YDQATQRFQVGLDAITSVYEAKAAYDQSIATVIAARNNQINQSENLRKLTNHVYETLA
                     PLKDSKIPLVKPEPNDVNQWIDTGLKQNYKLYAAKYNLEVAKDNVKAISAGNWPVFSL
                     QSNASQVHNNASGNTVFIPSKQTQANIAIAMNFPVFQGGLVQAQTRQAQYGFQSTSEK
                     LEQTYRDVVVNSRIAFNTITDGISKVKADRQTVISVQNSLQSTEAQFEVGTRTMVDVV
                     NAQQRLFEAQEQLARDQYDLINSILTLKYLAGTLNVNDLEQINSWLATTRVNGFSPVD
                     NKTSK"
     gene            17173..18024
                     /gene="ttcA"
                     /locus_tag="AC06IO_001646"
     CDS             17173..18024
                     /gene="ttcA"
                     /locus_tag="AC06IO_001646"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIO9874833.1"
                     /translation="MSSNPSSVEKKLLHYTGKAIADFNMIQRGDRVMVCLSGGKDSFT
                     LLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVK
                     EKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRS
                     MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLI
                     DQLAIENPKVPSNMLHALQSLKPSQLMDQNFWNFKNLEDGLETTHSIQCEEVFNAQEF
                     ELEDEKI"
BASE COUNT         5835 a         3975 c         3194 g         5172 t
ORIGIN      
        1 taacacgaat ttgtattaaa aatttctttg ttggactctg gttttctact ttgataatca
       61 ttcttgccag ttatttgctc gcaaaaaaaa gcatgagacc aattaaaata ttcactaaag
      121 aacttgcatc gattaatgct tcatcactag agaaacgatt accttacaca gggaatccca
      181 aagaactgga agaattagcc tcgacttgca atgacatgct ctttcgaata gaaagtgcat
      241 ttagccatat aaagcagttt tcagccagta tagctcatga attacgaaat cccattcatt
      301 acctcagaac tgccaccgaa attaccctga ctaaaccaca aacgattgaa acctatcaac
      361 atgtattaca aacgcatctc gaagaatatc aaagtttaac ccaacttata gataatctgc
      421 tctttttaac tcgctgtgag cacggccaac ttcaattaaa tttaagaaca ttatctgcaa
      481 accacttaat cagttcaatt attgagtatt attattcaat agctcaagaa aagggcatag
      541 aaattcaagt ttttggtgag gctcagattg aagtagatga acatttattt aagcgcgtta
      601 tttcaaattt aatagacaac agtctgacct atactaacaa aggcggaaga atactaatca
      661 tcatagaaaa aagaacagac gacagcattc aaatccgtat aaaagacaat ggaattggta
      721 tatcaaaaga acacttacct cttttatatc acggatttta tcgagtagat tatagtacta
      781 acaaagaaaa cgcaggcctt ggtttaggtt tggccattgc taaatcaatc atggagcacc
      841 ataaaggcca gcttgcggta gatagcaaga taggtttagg caccactgtt atattaaccc
      901 taccatgaaa aagcttttta aaattgaata atatatgtat ttcatctatc cgaaaccata
      961 agggatcgga tattgaacta agttattcta agaacataga gttttcacct cacgcagcac
     1021 cactaatcta caattaagga gagttgttag aataattatc aattaactca gatttaactt
     1081 ctacataccc tacgatttat ccaccctgct aaaagaatag tagaaaccac gcctaaatat
     1141 acgccataat gcttccaaga aggtctaact aattgcaatg catctgggct atgaaaaata
     1201 gaaaatggta tagcaagtaa cacatccatg atcgctgccc ctgccactaa accacaggca
     1261 ataagagtac ccttttgttt cctaatcata gattcttcag gtgccatttt tacacgccct
     1321 aattgctttt gaacaaataa ggctatcatt cctccaataa ataaaggaac cgaagaggaa
     1381 agaggtaaat acataccaat ggcaatacct aaaatagata aattaagaaa gcgtttgatg
     1441 tttaatatgc gattgagaaa aatggccaat aaaataatac atgaaccagc caacatcata
     1501 ttccagggca atgaatttct aaatactgct tccgtgatgg ctgccattaa tgcggcagtc
     1561 ggggcgggta acgattgtgt tacatccatt ccagcatgtg gcattactcc tgctatgcca
     1621 tagacattaa ataaaatctg catgacggga ggaataacca aagcagaaat aacaactcca
     1681 agcaacaaca tcacctcctg tttccatgga gtagccccaa ctaattgacc tacttttaag
     1741 tcttgggtgt tgtcgtttgc gatagccgct atgcctgtga ctacagctcc tataataata
     1801 gtaatagctt ctgcagcttg aatttgatgt gaagttaatg gaagtggcaa tagatgatta
     1861 attgctgaaa ctaataacca tgcggcaaaa agcatacccg agataaccac tgaactccca
     1921 ggacttgcag tgactcctac cattcctgaa aaataagctg taatcactga gaataaaaaa
     1981 ccaacgacta aaacataaat aacaccaata aatacaatag ttggtgaaaa atcattatcc
     2041 agtccaacct gccctaatgg aaaaatgatt tgaaagaaca aaaacagtat tcctgccatc
     2101 agtccaattc ccatcaatat aaatggcatg ggaatatctt tttcagtgcg aggatattgt
     2161 tctctgaact tgctcttgga aatataagtt ctgaatgatg tcttgacact tctggtgagt
     2221 ggtttcacta attttataaa agtccaaata ccggcaaaca gcatggctcc tatccccaaa
     2281 tagcgcattt cactattcca taacaaagta gctgcttgtt cagcgggata ttgatttaaa
     2341 aaatcaggat aaaactggct ggctattggc aaagcaatca accatgaaat gactgccccg
     2401 agaaaaatgc ttattgccat atcgtgacca acgaggtagc ccgcacctat catagttgca
     2461 gagaagccag cacccaaacc aaatagagaa cgttttacaa caaaccaata attccagctg
     2521 ctggcgatta ctttgaaacc aatttgcagt aactcgagaa gtccgcctac aagcccaccg
     2581 agaaaaatgt ctttaatacc caccctgtct gccgaagatt tcaagacctc tgcaatcgct
     2641 cttccttcag gaaatttgag ggtttggtca tttacaagaa ttcgacgcaa aggaatagaa
     2701 aacaaaactc ccaggattcc tccacaaaca gcaataaaaa aattagtgac gtaatcaaag
     2761 tggttccaaa acccgatgat aatgagcgca ggaatagtgt aaacgatacc gcctgcaaca
     2821 gcctcaccag cagatgctgc tgtctgtacg gcattgtttt caagaatggt tgaattttta
     2881 aaaaaccgca aaatccccat ggaaataata gcggcgggaa ttgaagcgga agttaatata
     2941 ccgagtttaa gcgccaaata agcattagac atcgccagta gaacggtcaa aattatcgcc
     3001 agaataatca ctctaaaagt gagttcagct acttttttat tcgctgcaat aaatggagta
     3061 tcagacatca attaccccac actgctttgg aaatttctgg ttttaccata gcagaaatta
     3121 gtgataatgg gatatccact gtttgctctc cataaacata agctgccacc tggtatgtat
     3181 taaaaacaat agcaatccct tttttggtaa aatgccaaat actataattt tcctgtgtcg
     3241 gtttcgttcc ttctttaatc cagttttcat ctgaaatctt cttggctgtg atttcttttt
     3301 tagccagatc agcgattggc tgcaaataat cggaacctgg aacaaataaa tcagacaatt
     3361 gtacaggctg attcttgata aagttgagta cctttactgc atttgaggga tgagcggctc
     3421 ctcgatgata gatggaaata ttaaatcgaa cacttagggc attagttgat tggtaaggaa
     3481 tagcatatgt aatatttaaa ccggttttac ctggagcatc tgcaggagta tcagcatctt
     3541 cagataattc attcataaaa ctcttttgct gattagtgat aaattccttt accgcctgat
     3601 tgactccgtt actttgaaat ccctgagggt attttatatc cagcaaatat tccgctgttt
     3661 cttttttaat aacttctgtt gtaatcgcag aagcaagaaa cgaataaaat aatagcccaa
     3721 acatcaggcc aattttattg attaaattca tttgtatcgg cattgtttat ctccaatcaa
     3781 gtaatcctct caggataccc attcaccagc caccatcgtt tgatgaggta acggataagc
     3841 gaaataataa catagcgcag cgctatcttt tatagaccat aaaaccaaat cagcaatctt
     3901 gcctgcttca atgctcccaa tatccttttc cattcctaaa gctctggaag cctgatacgt
     3961 tactgcagat aaaacttcag gtatagacat agagaaaaat tggcaagcca tactcatcat
     4021 caataacaaa gaagtcgttg gagaggaacc cgggttagaa tccgtggcaa tggccatacc
     4081 aacaccaacc tgacgcaata aatcaacagg tggtttttgt ttttctttaa ggaaataaaa
     4141 agcgccagga agtaagacag caactgtatt agcttttacc atatttaaag cgccgttttc
     4201 atccaaaaac tccagatgat cacaggataa cgcaccataa cgagcagcca aagaactggc
     4261 ccccatatta gataattgtt cagcatggca ttttattggt agatttaaat tcttagcagc
     4321 ttgaaaaatt tgctcagcct gccttataga aaaagctatg gattcacaaa aaacatccac
     4381 ggcatctacc aaacccatat ttttagccgc aggcaacatt tcattacaaa gaaaatcgac
     4441 ataagcctga ctatttcctt taaactcggg acctacagca tgcgccccca gaaaagttgt
     4501 ttttaccctg actccagccg tgtcccctaa ctgcctggca accctgagca ttttcaattc
     4561 attttgcaaa tcaaggccgt agcctgactt aatttcaacg gtagtaaccc cttcattttt
     4621 tagcgcaaga agtcttggca atgattgatc aattaactct tcttcagagg catctcgtgt
     4681 catttgtact gtggataata tgcccccgcc tgatttggcg atatcagcgt aactgacgcc
     4741 ttgcaatttt aatcgaaact ctgcggctct gtggcctgca taaaccagat gagtatgaca
     4801 atcaattaat cctggtgtaa ttaattggcc gtgacaatcc tttcttgact tggcactttc
     4861 ctgaaaatgg acaggtagct ggtcctcaga accacaccac tctattctgc cttttcttat
     4921 aacaatggct tgattagaaa gttgcaaccc tgtggcatcg attgtgcttg cattgagcaa
     4981 taattcatca caggcaaaca ttaaaattcc caatgaagta cctggtgagg cggcttcaac
     5041 caggcagagt ggtgagtcgt gtcatatata tcccactctt tggatacata cgtttcatta
     5101 tcaatatcta acagcagcca aataaaaaat tctgctactg tttcgggtga tatcaaacga
     5161 ttatgatctt ttaaacgtct ataaaaattg acctgttccg gatcaagatt aatcccgttt
     5221 cgggctatcg cctgcatatg agtgtcgata attccaggca ttacgctggc aaaggccagt
     5281 gaatcgcact caatctgcca gcatcgtgtt aacatagcca aagccgcttt cgatacacaa
     5341 taagcccccc atcctttgat aggaaaataa gcggcagctg aacctacatt caatactctg
     5401 ccacctgaca atttatcata caaaagttgt ggaagaaaaa gtgccgcgtc tatattggtg
     5461 tttaatgcct ggtgccactc ggcaaaagac atatctttta aaagcagtgt aggctccaga
     5521 gttcctgcat tgtttatcaa accatcaatt tgaggtgtgg cacttaaact ggctttgatt
     5581 aactctctgc cttgcggggt ggagacatcg gcacataaat aatgaatgtt tgcatataag
     5641 gaagcggttt cttgcagtaa tttttccctg cgccccacta tgaacacctg tttattgcgt
     5701 tttgccaaag caaaggccaa agctcttcct atgccgctac caccaccagt aatgagatac
     5761 acaatttcct catcaatata gaaaacgata ggtgaaataa taccagacat tagacttctt
     5821 gcataacctg cacgttttat tttagaacaa gaaaaaagcg actgaacgac ctaattttat
     5881 taaaaataaa tgaaaatctt gagtatttag catttctctt ggaaactagc cctatcatta
     5941 gagctcttgc ataacctgat tattttgttt taggacaagg ggcaagcgtc ttgaggagcg
     6001 gagtttaaat atagctgagc acccagaaac gttcgcaaca cggcattaaa actaaagaaa
     6061 caggttatgc aagaggtcta ttaaaaggag aacttgatgt ttgacgattt aagtaactat
     6121 cggccagcca accctgcgct ttggcaaggc aggaaggaca ccgccaacca ggaacgtttt
     6181 tttcaaaaaa ttacttttat tgataatcaa aatgaattaa tgactaaaga caaaaaaacc
     6241 atatttcttg gatttgccag tgatgccgga attaaacgca atttaggcag aacaggagca
     6301 aaattaggcc ctgatcaaat taaaacccaa cttgccaaat taccatgtca taataataaa
     6361 cattatgttg atcttggaaa cgtgatatgt gaaaacgatg aattggaatt atcccaatct
     6421 caatttgctc aaatagttca tttttgccat gaaaatggcc atcagatctg tgctttcggc
     6481 ggtgggcatg aaatagcctg ggcgcactat caaggattgt cctcactcta tccgaaatta
     6541 ggggttatca actttgatgc tcattttgat ttacgccctt ataaaaaagg tgagtttggc
     6601 aactcgggaa ctcctttctc tcaaattgcc gcctattgcg aagaaaaaaa aatgcctttt
     6661 cattattgtt gtattggggt gcaaaaattt ggcaataccc catctctttt tgaaaaagca
     6721 aaaaaattaa atgtgagcta cctctcagca gaagaccttt atgagcaaag ccaggcttgg
     6781 caaattgcat ttcttgatga ttttatcctt aatttggacc atatttactt aacgatatgc
     6841 ctggatgttc tcgccgaatg ctatgcccct ggtgtaagcg ccccgcaagc attgggttta
     6901 tctccctggc aaataatgcc actgttgaaa taccttatac aaagtggcaa agtagtaagt
     6961 ttagatattg cagaactttc gccgccactg gattccgaat taaaaactgc tagactagcg
     7021 gcacttatta tcgcagaatt gttagatact aattgaggag tatatttcat gaagaaaact
     7081 gtgctgtggg gtgctttatt tactctaatc actactcaag cgaactctga agcaactcag
     7141 caagcgatat caactcaaaa ttcggcgtcc accggcacta atgtagtttc tgagccaacg
     7201 aatcaggtac ctgccacgaa tcaaaacacg cagcaaaatc agctgaacca acctgtacag
     7261 tcaaatggac aacaaccaac tcaagccatt caaaacaatc aacaaaatca accgagccag
     7321 acaggggaac aacagtctaa ccaaaacacc cagaataatc agcagaatca accagcacag
     7381 caacaaacca cacagcaacc tgctcaacaa caagtacctt cacaacaaac agcgccagtt
     7441 attaattgtg attacaagat ttccgctgaa acaaaagcaa tagagcaatc cctggtttta
     7501 acctggtcag agaaggcaat aattcagtca tttgattttg atccggctac tgtagatgcc
     7561 caattgcaaa aattacaagc ctgctttaca gatcaaggat ggctaagctt taattcagca
     7621 ttgcaaaaat cagggaatct cgatgccatt aaatcacaaa agttgcatgt aagcagccaa
     7681 attgatggtc agccacaagt gactgaagcg atagaaaatc aatggaaaat tatcatacca
     7741 ttacaagtcg tctatcaaaa tgacaaggag aaggtaaccc aattacttaa tgtcaacgta
     7801 actgttggac gaaaaattac cggtgattta ggcatcaacc aaatgattgc tgtaccccgc
     7861 acagcaacca atacttcaca aaccaattca ccgtctaata caaacccagc agactcaaat
     7921 acctcgacct ctcaaacacc tggtgacaca acaaataatg ctacccaaac caatacacaa
     7981 acacagactg ggacaccaac caactcacct ccgagtggct ctacaacaaa tacccagaat
     8041 taaattcaac aattagtgca gcattccatt aagaatgctg ccaaaatcac ggaaatatca
     8101 tgcgattaat atttacagga gtattatgcc tgttgactct caatgcccag gcgaatgtga
     8161 tacaatattt tgcaggaatt agttataaca accctgctga cttattcaaa gtaaaaaatg
     8221 gtatcttgct tattggagga accggttctt atgctgactt gcaatttaag ggaagcgcct
     8281 taaattttaa tacgtttcaa tacgactcag gagttaatca ctcgcgtact tatattgttt
     8341 ggccatatgg tcgagttgcc aagcgcctca atgataagac tgtagttgca gtagatctta
     8401 ccgaaccttt taattccaat cttgattggg gaaacgacgc ttttacaaga tatgctgcga
     8461 cccaaaatta cctgaccgat gtggatctaa gccccaaaat atcctatgcg attagcaaaa
     8521 aattacaaat tggtggtggc ataaacttta atgtactgct aaagaatgaa gtcaattggg
     8581 cttttcctac tggccaatcc acttatgcga atttaattaa ccgatcctct agttttggtg
     8641 tgggttataa tttggggatt aattatgcag tgaatgatac caatttcctt ggtatcactt
     8701 attattcaag aattcgccaa aatacttccg gaaccagtta tctgggatta gctgccaacc
     8761 ctgatttcca attcggtttt tatatgccag caacaacggt tgccagttat gtccatattt
     8821 ttaatcccaa atggctcatc aacctacagg tttttcaaag cgaatggaac gccaatcaaa
     8881 aagtgagact ttataacaca gcagcaccac ctccatttac caattttatt tttgatatgc
     8941 atttcgatac ctcttatgct tatcttgccg cgatacgtaa acaagtctct gataaattag
     9001 gcattgcgct ggcaggcatg atcgatgatg gtcctgaaga agatggcctt agaaccatcg
     9061 ttttcccttc tgatactcaa tattttcttg gattgattgg tgactaccgt tttacggaac
     9121 atgcttccat ggaacttatc ttgggacatg tgtattctaa tcccagcatc caaaacaaag
     9181 ccaaggtaaa caatgtaccc gttcccttta ctacaggccg ggtgacaatt aatgccaatg
     9241 ttttggatct taaagttaaa atcgaggggt gatcttccag gtgtaaccac ctggcacttc
     9301 ctccaagagt tttattgttg aatagtcttc tttttaaata gccaataata ggtccatgat
     9361 cctgctatca gcccaatcac aaaatatacc ggatcggaat taaaggtagt taaggcacta
     9421 atagatgggc ctgggcagta tccagccaca ccccaaccaa ttccaaaaat gatacttccc
     9481 aagattaatt ttttctcaat gcttttcttc tcgggtaaat aaaattgatt acacaatatt
     9541 ggtttatcaa atttgtttat cagtttgtac cccaaaaaag ttacggtgac agcaacagcc
     9601 attacaatca gtaaactcgg atcccaaaat ccattgatat caagaaaatt taataccttg
     9661 ttaggattga tcatatttga aatagtcaac ccggctccaa ataacaaacc acatagaaat
     9721 gacatgatgt tatacattaa tataatcctc ccacatgtct tatcagatac acagtaatca
     9781 ttgcagataa gataaaaagc agtgtcgcta taaaggagcg cttggaaaat ctggccagtc
     9841 cacatacccc gtgtcccgaa gtacaacctt ggcccattcg ggttccaaaa ccaaccaaaa
     9901 atccaccaat tagtaacagg aaaaccggat aatgtgttct taatggaaac tggataacag
     9961 ggcttatgaa atagatcaat cctgcagctg ccaaaccaag taaaaaggca atacgccaga
    10021 ctgggaatgg tttttcaggt ggacagagtc cgtgtatcat tccactgatg ccagctatac
    10081 ggccgtttaa ccataaaaaa aggacagcac ttaagccaat cagtaatcca ccaatggcac
    10141 ttagataagg agtaaaagag gttatataat ccattattta tatttccgtt tcatttacaa
    10201 tgaataacac ctgaattgag acactttaat tttttaaagt gtctctcaaa ttatattgat
    10261 gttttaaaac aatagtccaa attttaattc actttttcta atgaggacat ggttttcacc
    10321 ccgttttgat cagggtaggc taatacgatg actttcgttc caactcgcaa gaactcttcg
    10381 ttcaaccatt tggctgcact ggggaaaacc ctgacacaac catgacttga tgggtgctct
    10441 ggaacttcat aagccgcgtg aatagtgaat cctctgtaaa aatacatgca gtaaggcatt
    10501 ttagccccac ctttcgtatc gacagggtat tcacccgaac gacagtcaat acctcgtttg
    10561 ttataaatcc tgaatgttcc ggttatcgtc ctgcaagatt gatttggatt ttcttcgcaa
    10621 acatctattc caccagaacc agctcctgtc attacacgat gcccctcttc atcgtaggct
    10681 gcccaggcag acgctttggg atcaaaaaca aataatcttt cgcctggcgc tttaatttgc
    10741 attgggaaat aattacgtcc tcttttatcc cttaaataat gaacggttct gtgtacataa
    10801 ccagcatcgt cagtaattaa ggttgactca tcaaactcaa cacacgaagt taatccgagg
    10861 cacattaaaa taactgccca ataaattttt ttcatttaga ttttatctcc acaatagtaa
    10921 aacctggtgg ccctaattaa tcacttaatt ttcgatattt aattcgtgac ggtctgttag
    10981 cctcatcacc caatcgacgc tttctatccg cttcgtattc actgtaatta ccttcgaaaa
    11041 aggttatttg tgaatcgcct tcaaaagcca ttaaatgggt acaaattcta tctaaaaacc
    11101 atctatcgtg agaaatcact atagcacaac ctggaaaatt aagaatggcc tcttccaatg
    11161 ctctcaatgt ctcaacatcc aaatcattac tgggctcatc gaggagcaaa acattcccgc
    11221 cacttttcaa caattttgcc aaatgaaccc ggttacgctc tccaccagac aattgagaaa
    11281 ttttcttttg ttgatccgtc cctttaaaat taaaacgccc aacataggct cgcgaaggca
    11341 tttggaaatt acctatctgc ataatgtcat ggccatctga aatttcctgc cacaccgaat
    11401 tattgggatt tagattgtcc cgcatctgat caacataagc caacttcact gtttctccaa
    11461 tacgaatgac acctttgtct ggctcttcct gcccggttaa catttttaaa aaagtagact
    11521 tacctgctcc attaggacca ataattccta aaataccgcc tttgggaagt ttgaaatcaa
    11581 aattatctat taatatcctg tcgccgtagg atttgcatat tttttcaccc tccagaacca
    11641 aatcacctaa ccgttcaccg ggcgggatat atatttcatt ggtttcatta cgcttttgga
    11701 attccttgga attcatttct tcaaatcggg ccaagcgggc tttattttta gcatgacgac
    11761 ctttgggtga ggttctaacc cattccaatt ctgctttaat ggcacgttgc aaagcatcct
    11821 cctgcttttt ctccatactc aaccgtgctt ctttttgttc gagccaggaa ctgtagttcc
    11881 ccttataagg gataccttca cctcgatcca attccaaaat ccattccgct gcattatcca
    11941 ggaaatacct gtcatgggta attgcaacta cagtgccagt aaactcttca aggtaatgct
    12001 ctaaccaggc aacactttct gcatccaaat ggttggtagg ttcatcaaga agcaacatat
    12061 caggacttga tagcaataat cggcaaagag caacacgcct gcgttcacca ccagacagtt
    12121 tttctattat ggcatcccaa tcaggtaatc ttaatgcatc agcagccaca tcaagctttc
    12181 tctccaggtc ccaaccgcca caagcttcta tctcattttg taactcacct tgctctgtta
    12241 acaaggcatt catctcatca tcgctcattg gttctgcaaa acgcatacta atggcatcga
    12301 aacgagacag tttttcttta atttcagcga cgccttcctc gacgatttca cgcacagttt
    12361 tattcaagtc aagctcaggt tcttgagcaa gatacccgat tttaattcct ggttgaggtc
    12421 ttgcttcgcc ttcatattgt gtatcaacac ctgccataat ccgcaataga gtagattttc
    12481 cagaaccatt tagtcctaat acaccaattt ttgctccagg aaaaaaactt aatgaaatat
    12541 cctttaaaat aaatctttgg ttctcaacaa tcttgctcac tctattcata gtgaatatat
    12601 actgcgccat aatcgctcca actctctata atcagccttt tatcaaacaa tactaccagt
    12661 tcagtatgac ttttcctgac tgacccgatg ccataatttg aaatgcgtgc tgatattcat
    12721 ctactgggaa attatgagta ataacagggg aaatatttaa accactttgt agcatagcaa
    12781 tcatcttata ccaggtttca aacatttctc gcccataaat acccttaata accaatccct
    12841 tgaaaataac ctggttccaa tctatcgggg tttcttgtgg tggaatacct aataaagcca
    12901 catggccacc atgattcatc gctttcatca tgtcgtttaa tgccatagga tttcctgaca
    12961 tttccagacc aacatcaaac ccttcgagca ttcccaactc attagccaca tcacttaatt
    13021 tttggtattt gacattaacc gctcggctga cacccatttg cctggccagt tccaatcgat
    13081 gatcgttaac atcggtgata acaacatgcc gtgcgccaat atgtcttacg atggctgcgg
    13141 ccattattcc tatggggcct gcaccagtaa tcaaaacgtc ttcgcctacg acatcaaatg
    13201 ccaatgcaca atgagcagca tttccaaaag gatcgagaat agccgcttgc tcttctgtaa
    13261 tgttgtcagg gagagcaatg acattggtag caggaagcgc cagatactca gcaaagcatc
    13321 ctggtcgatt cacccccact cccagggtat tacgacataa atgacgcttg ccggcacgac
    13381 aatttctgca aaaaccacag gtaatatgac cttcaccaga aactctctgt ccaactttca
    13441 aaccttgtac ttcttttcca acctctacta tttcaccata aaattcatgc cctacggtca
    13501 taggaacagg aattgtggct tgcgcccatt catcccagga ataaatatgg atatctgtac
    13561 cacaaattgc agtcctttta atttttatta aaacatcgtt tactccatat tcaggaacag
    13621 gaatatcctg catccaaatt ccaggctctt tttttgcttt aaccaatgat ttcattgcca
    13681 tacctcatta attaggtaat accaccattt cgcaattcac cctaaaaata tcaggttgtg
    13741 aaatgtagag taatttagtt tcagattact gagaattcct taccaaccgt ttcaaacgcc
    13801 ttcaaggctt tatccaaatg atgtaattca agagccgcag acatttgggt acgaatgcga
    13861 gccaaacctt tgggtaccac tggataggaa aaccctacaa catagacccc caactccagc
    13921 aaccgattag cgattcgccc ggccaaactg gcatctccta acatgacagg aataataggg
    13981 tgttcccccg ggattaactc aaaacctaac tgagtcattc cttcacggaa ataatgacta
    14041 ttgcgcttta atttttcagc aagggagtta tttgtggaca atttatcgag caccacacaa
    14101 gaagtatgcg caataacagg cgccaatgta ttggaaaaca gataaggcct ggatcgttgg
    14161 cgcaaccaat caatgacaac ttgatttgac gcagtatatc ctcctgatgc gccccctaag
    14221 gctttaccaa gagtgccagt tacgatatcg attctgtcac taactccaaa atgctctggt
    14281 gttcctctac ctgtcttacc catgaaacca acagcatgag aatcatcaac catcaccatg
    14341 gcatcatact tgtcggccag ttcacaaatt gctggcaagt tagctaatat accatccatt
    14401 gaaaaaacac catctgttgc aatcaaacga aatctcgcat ttttcgctgc aattaactgt
    14461 tcttccagag ccttcatatc attattggca tatcgataac gtgcggcctt acacagcctg
    14521 actccatcaa taatactcgc atgattcagg gcatcgctga taatggcatc ttcttctgtc
    14581 aataatgtct caaataatcc cgtattggca tcaaaacagg aagaatacaa tatagtgtct
    14641 tctttactta aaaaatggct aattttttgt tccagttgct tatgcggggt ttgtgttcca
    14701 caaataaaac gaacagaagc catgccatat ccgtacttgg ctaacgcagc ctgtccctcc
    14761 gctattagtt caggatcatt ggccaaaccc aagtagttat ttgcacaaag attaataact
    14821 tctctttcgt taaccttcac gtcagcttgc tgttggctgg aaataactcg ctctgattta
    14881 tacaatccgt ctgccttgag tgtttcaagt tcattttgta aataattaat gaatcgctca
    14941 agcactataa tctcctgtat ttctggataa atttgtaaat aataccaaat tttaaacatt
    15001 ctcaccatga gatattatca agaaaatttc actttcaaac attaatgctt agacataagc
    15061 tgaattcttg ctaaaattgc tgcaaagttt taacctatcc aatgattatt aagacggtag
    15121 agcaaatgaa tcacagtgca cgcataaata tgatcaaaca acagttacga actggtgatg
    15181 tattaaatga atccatcctc gacttatacg atgagatttt acgacatgag tttgttccag
    15241 agcttttctc taattttgct tattcagaca tgcaaattcc tttggcacat ggccaaagga
    15301 tgttaactcc attggaagaa ggaacaatta tacaagccct tgatcttaaa ggccatgaaa
    15361 ccgtgctgga agtaggaaca ggaaccggtt ttatgactgc tttactcagt aaattatgta
    15421 aaaaagtaat cagtattgac tattattctg actttactgc caatgcaaaa cgtaaacttg
    15481 aagagaataa ttgtaataat gttgaattaa tcactggaga tgcttgtcgt ggatggttgg
    15541 aaagtgcgcc atacgatgtt atggtattta ctggtgccat ggaaaaactg acagataccc
    15601 ataaactaca aattttaccc ggtggcaagt tgtttgcgat ccttggtaaa tcccctgtaa
    15661 tgcaagctta tctttttcag ttggatcata atgcaatctg gacagaatcc atgctttttg
    15721 aaacggatat acctcctctg gtagatcaat tgaaaccgaa agaatttgtt ttctagggaa
    15781 aactcgaatg agaaaatctc tgttttgttg gattttaact ttaggtgttt caacccatgt
    15841 gtttgcaaca gacctaatgg atatctatca acaagctctg gagaacgata ccatttttaa
    15901 agaggcatat gatacgtata tgtccagcac agaagccata cctcaagctc gtgccgctct
    15961 ttaccctcaa gtaggacttg gttcacaagc tggtcgcaat tatcaggatg ctgttgctgg
    16021 agctttcagt gccaatcaat actatggaag ttatttgtgg caagtcaatg cttctcaggc
    16081 actgtttaac tatcaggcct gggccaaggt agcccaggct aaagcctctg ttaaagcagc
    16141 acaagccact ttcaatgatg cagcacagaa tttaattcta agaacggcta aagcctattt
    16201 tgatgtcttg tttgctaaag atactttaga ctttgccgaa gcaaaaaaac gagcgaacaa
    16261 aagacaatat gatcaagcaa ctcaacgttt tcaagttggc ctggatgcca tcacttctgt
    16321 ctatgaagca aaagcagcgt atgatcaatc catagcgaca gttatcgccg cccgcaataa
    16381 tcagattaat cagagtgaaa atttacgaaa acttaccaat cacgtatacg aaacacttgc
    16441 gccactcaaa gacagcaaaa ttcctcttgt taagccagag ccaaatgacg tgaatcaatg
    16501 gatagatacc ggacttaagc aaaactataa attatatgct gcaaaatata atcttgaggt
    16561 agcaaaagac aatgtaaaag cgatctctgc gggcaactgg ccagtttttt cactacagag
    16621 caacgcaagc caggtacata ataatgcatc cggcaatacc gtttttattc cgtcaaaaca
    16681 aacccaggcc aatattgcaa tcgcgatgaa ttttcctgtt tttcaaggcg gcctggtcca
    16741 ggctcaaacc cgacaagctc aatatggctt tcagtctacg agtgaaaaac tggaacaaac
    16801 gtatcgcgat gtcgttgtaa acagccgcat tgctttcaat accatcactg atgggatcag
    16861 caaggtaaag gcggacagac aaacggtcat ttccgtgcaa aattctttgc aaagtactga
    16921 agctcaattt gaagttggta ctcgaaccat ggtagatgtg gtcaatgctc aacaaagatt
    16981 gtttgaagca caggaacaat tagcccggga tcaatacgac ttgattaatt ccatacttac
    17041 gctgaaatac cttgcaggaa cattaaacgt taatgatctc gaacagatta actcatggtt
    17101 agcaactaca cgtgtcaatg gattttcacc cgtagataac aaaacctcca aataacaaat
    17161 ttaggttttt ttatgtcttc caatccttct tcagttgaaa aaaaattact tcattataca
    17221 ggtaaagcca tagctgattt taatatgatc cagcgtggtg acagagtcat ggtatgtctg
    17281 tctggcggaa aagattcatt cacactatta acaattctta accagctcag gatcaaatca
    17341 ggaaataaat ttgaaatttt tgcgtttact ctggatcaag cccagccagg gtggaatgat
    17401 gcctgtttgc gtcaatggtt ggctgaaaaa tccattcctc atgaaatact aacccgagac
    17461 acatacagta tagtgaagga aaaaattcct gaaggcaaaa cctactgttc tttgtgttct
    17521 cgcttgagac gtggaatcat ctatagatat gctgaagaaa acggatttaa taaaatagcc
    17581 ttaggtcacc atcgtgatga tcttattcgt acattgatga tgtcaattct atataatggc
    17641 gatatacgtt ccatgccacc caaattatta agtgataata aaaagcacat agtcatccgg
    17701 ccactgtgct atgtacagga aaaagatatt attacgtttg catcagaaca agcctttcca
    17761 attatcccat gcaatctttg tggttctcaa gaaaatctaa tgcgcaaaaa agttgcctca
    17821 ctcattgatc agcttgcgat tgaaaacccc aaagttccca gtaacatgct tcatgcgctg
    17881 caaagtttga agccaagtca attgatggat caaaattttt ggaattttaa aaatctggaa
    17941 gatgggcttg aaaccacgca ctcaatccaa tgtgaagagg tttttaacgc acaggaattt
    18001 gagcttgagg atgaaaaaat ttgacttatg aaaattatta tctaatattt cttttcattt
    18061 ttgcaatata atgaaataat tggcacgtaa aataagctta ttattttgac aaatggaatt
    18121 aaatatataa aaataggctt tcaaattatc acattacctg tgactttgtt tcatta
//
LOCUS       DBNCWK010000056        18112 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.056, whole genome shotgun sequence.
ACCESSION   DBNCWK010000056 DBNCWK010000000
VERSION     DBNCWK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18112
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.056"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..997
                     /locus_tag="AC06IO_001647"
     rRNA            <1..997
                     /locus_tag="AC06IO_001647"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1121..1236
                     /gene="rrf"
                     /locus_tag="AC06IO_001648"
     rRNA            1121..1236
                     /gene="rrf"
                     /locus_tag="AC06IO_001648"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1343..1418
                     /locus_tag="AC06IO_001649"
     tRNA            1343..1418
                     /locus_tag="AC06IO_001649"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1376..1378,aa:Thr,seq:tgt)
     gene            1491..1575
                     /locus_tag="AC06IO_001650"
     tRNA            1491..1575
                     /locus_tag="AC06IO_001650"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1525..1527,aa:Tyr,seq:gta)
     gene            1596..1669
                     /locus_tag="AC06IO_001651"
     tRNA            1596..1669
                     /locus_tag="AC06IO_001651"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1628..1630,aa:Gly,seq:tcc)
     gene            1722..1797
                     /locus_tag="AC06IO_001652"
     tRNA            1722..1797
                     /locus_tag="AC06IO_001652"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1755..1757,aa:Thr,seq:ggt)
     gene            1832..3022
                     /gene="tuf"
                     /locus_tag="AC06IO_001653"
     CDS             1832..3022
                     /gene="tuf"
                     /locus_tag="AC06IO_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIO9874834.1"
                     /translation="MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTA
                     KAYDQIDAAPEERERGITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSS
                     YDFPGDDIPIVVGSALKALEGEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIE
                     DVFSISGRGTVVTGRVESGIVKVGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDN
                     VGVLLRGTKRDEVERGQVLAKPGTIKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFY
                     FRTTDVTGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVA
                     KIIE"
     gene            3082..3157
                     /locus_tag="AC06IO_001654"
     tRNA            3082..3157
                     /locus_tag="AC06IO_001654"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Trp,seq:cca)
     gene            3171..3542
                     /gene="secE"
                     /locus_tag="AC06IO_001655"
     CDS             3171..3542
                     /gene="secE"
                     /locus_tag="AC06IO_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIO9874835.1"
                     /translation="MKSSNENQGKMKDIMSWLAIILATAGAFVCTYYYTFTGPIQSII
                     WLAWLILILFLGYLTTTGKRVFVFAQEAKVELLKVVWPTRQETIQTTTIVMVMVGLTG
                     FILWGVDSIMMWAIAKLTHLG"
     gene            3545..4093
                     /gene="nusG"
                     /locus_tag="AC06IO_001656"
     CDS             3545..4093
                     /gene="nusG"
                     /locus_tag="AC06IO_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIO9874836.1"
                     /translation="MVEENKAKQWYVVHAYSGYENFVMREITSRAKHHNLQDKIGEVV
                     VPSEEVVEMRSGQKRKSTRKFFPGYVLVHMVMDDQTWHMVRGIPRVLGFIGGTSQTPT
                     PISDKEAQAIMQRVEDGVTKPRPKILFEPGEVIRVKEGPFVDFNGVVEEVNYEKNRLR
                     VAVLIFGRSTPVELEFSQVEKT"
     gene            4204..4638
                     /gene="rplK"
                     /locus_tag="AC06IO_001657"
     CDS             4204..4638
                     /gene="rplK"
                     /locus_tag="AC06IO_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIO9874837.1"
                     /translation="MAKKVEAYIKLQIPAGKANPSPPVGPALGQRGVNIMEFCKAFNA
                     ATQQMEQGLPIPVVITVYSDRSFTFITKTPPASVLLKKAAGIQSGSGTPNTKKVAKLN
                     VSQLEEIAKVKKPDLTAADLAAAVRSIAGTARSMGIEVEGLE"
     gene            4648..5343
                     /gene="rplA"
                     /locus_tag="AC06IO_001658"
     CDS             4648..5343
                     /gene="rplA"
                     /locus_tag="AC06IO_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIO9874838.1"
                     /translation="MSKLTKKQKKIAEVIKPNQLYTVADAVAILKQFASKKFRESLDI
                     SINLGVDPRKSDQVVRSSTNLPKGTGKTVRVAVFAQGDNAAKATAAGADIVGFEDLAD
                     KIKAGEMNFDVVIATPDAMRIVGQLGQILGPRGLMPNPKVGTVTTNVEAAVNDAKSGQ
                     VRYRTDKNGIIHCTVGKADFAPEDVLENVVALINDLKKAKPASSKGQYLKKISLSTTM
                     GPGLPIDISSIPV"
     gene            5516..6049
                     /gene="rplJ"
                     /locus_tag="AC06IO_001659"
     CDS             5516..6049
                     /gene="rplJ"
                     /locus_tag="AC06IO_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIO9874839.1"
                     /translation="MTLNLAAKKAVVEEVTAVASKAISAVVADYRGLTVNQMTQLRSE
                     ARKSGVYLRVVRNTLTRRAFKNTEFECLNDLLVGPVFIALSLEAPSDAARLLKDYAKT
                     FEKLEIRALSVGGKVYNANQIDAVASLPTRDEAISKLMYVMKAPIEKFVRTLAEPHAK
                     LARTLAAVKDKKAGNPA"
     gene            6080..6460
                     /gene="rplL"
                     /locus_tag="AC06IO_001660"
     CDS             6080..6460
                     /gene="rplL"
                     /locus_tag="AC06IO_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIO9874840.1"
                     /translation="MAVSKNEILETISNMTVMEVVELIEAMEEKFNVSAAAAAVAVAA
                     PAAGAGAAAAEEQTEFTVVMTSFGSNKVNVIKAIRGITGLGLKEAKDLVEGAPSTVKE
                     GVSKDEAASIKKELEEAGATVEVK"
     gene            6552..10658
                     /gene="rpoB"
                     /locus_tag="AC06IO_001661"
     CDS             6552..10658
                     /gene="rpoB"
                     /locus_tag="AC06IO_001661"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIO9874841.1"
                     /translation="MAVAEAKPQYSHAEKKRFRKSFGKQTDIMPIPNLLEIQLKSYRD
                     FLQTDTKLSEQLNTGLHAAFSSVFPIESFSGNARLEYVGYKLGEPAFDVRECKLRGLT
                     YSAPLRVKIRLVVLDKDASDDPKPIKDIREQDVFMGEIPLMTDVGTFVVNGTERVVVS
                     QLHRSPGVIFEHDKGKTHSSGKLLYSARIIPYRGSWLDFEFDPKDCVYVRIDRRRKLP
                     VTILLRALGYEAEDILNEFFETTCCHLKNGEYHIDLIPQRLRGEIASFDIHVPETGEL
                     IVEQGRRITARHIKQMEKSQMQDLVVPRDYLIGKTLAKNIIDTSTGEFLAQANDEITE
                     ELLDAMASHGILQIDMIYTNDLDHGSYISDTLKIDPTGSQLEALVEIYRMMRPGEPPT
                     KEAAEALFKNLFFVEERYDLSAVGRMKFNRRVGIKSDEGPGTLTKEDILSVIKTLIDI
                     RNGIGMVDDIDHLGNRRVRSVGEMTENQFRVGLVRVERAVKERLSLVESENLMPQDLI
                     NAKPVSAAIKEFFGSSQLSQFMDQVNPLSGVTHKRRVSALGPGGLTRERAGFEVRDVH
                     TTHYGRVCPIETPEGPNIGLINSLSVYARTNEYGFIETPCRKVVNGRVTDEVEYLSAI
                     EEVDQYIAQSNVELDAQGNILADLVPCRHQNEFSLTTPDKINYMDVSPKQIVSVAASL
                     IPFLEHDDANRALMGSNMQRQAVPTLRSEKPLVGTGMERIVASDSGVSVVAKRGGVID
                     LVDASRIVVRVNDDETTAGETGVDIYNLTKYFRSNQDTCINQRPIVSTGDRIQRGDVL
                     ADGPCTDMGELALGQNLLVAFMPWNGYNFEDSILISERIVHDDRFTTIHIEELTCIAR
                     DTKLGTEEITADIPNVGESALSNLDESGVVYIGAEVKAGDILVGKVTPKGETQLTPEE
                     KLLRAIFGEKASDVKDSSLRVPSGMNGTVIDVQVFTRDGLEKDARAKSIEEEHLARVR
                     KDLIDERRIREEDIYHRVSHLLLDKVATGGPGSLKPGSKITQDYLDKVEREKWFDIRI
                     EDDAISQQLEQLSKQLELLTKEMEKRFNDSRKKIIQGDDLAPGVLKIVKVYLAVKRRI
                     QPGDKMAGRHGNKGVISIVVPVEDMPHMEDGTAVDIVLNPLGVPSRMNIGQVLETHLG
                     LAAKGLGRKIAQMLDEKQTPEAIKAYLEKIYNHDGVQRVNLKCLNDDELMTLADNLRA
                     GVPMATPVFDGATEQEIKSMLQLADLPADGKTVLIDGRTGNKFDNPVTVGYMYMLKLN
                     HLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVK
                     SDDVGGRTKIYKNIVDGDHRMDPGMPESFNVLLKEIRALGIDIELEHD"
     gene            10747..14952
                     /gene="rpoC"
                     /locus_tag="AC06IO_001662"
     CDS             10747..14952
                     /gene="rpoC"
                     /locus_tag="AC06IO_001662"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032829172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIO9874842.1"
                     /translation="MSDLLGILKQQGQSEEFDAIKIALASPELIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKTFGPVKDYECLCGKYKRLKHRGVICEKCGVELALAKVRRERM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFEAFVVVDPGMTELERGQLL
                     NDEAYLDAMEQYGDEFDARMGAEAIRDLLRQIDLEDEIRNLREELPTTNSETKIKKIT
                     KRLKLLEAFYESGNKPEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLNAPDIIVRNEKRMLQESVDALLDNGRRGRAITGTNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKKMALELFKPFIFSKLEFRGLAT
                     TIKAAKKMVEREESVVWDILDDVIREHPILLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCTAYNADFDGDQMAVHVPLTLEAQLEARSLMMSTNNILSPASGEPIIVPSQDVVL
                     GLYYLTREKVNALGEGKIYSSAQEAQNFYEAGHLDIHAKIKIRMPKEDGETGYHLVET
                     TVGRAILAEILPKGMPFDYINRTMTKKVISKVIDSCYRKFGLKETVIFADQLMYTGFK
                     YATRSGASIGIEDMEIPDDKASIIEHADNEVREIESQFRSGLVTNGERYNKVIDIWSR
                     TNELVAKSMMSKIATEEVTDAKGNKVRQESFNPIFMMADSGARGSAAQIRQLAGMRGL
                     MAAPDGSIIETPITANFREGLNVFQYFISTHGARKGLADTALKTANSGYLTRRLVDVA
                     QDVVITEDDCGTDTGILMQPLIEGGDIVEPLHERVLGRVVASDVYIPTQTEPVVKAGT
                     LLDEEWVEKLEKHGVDQVMVRSPITCQTRFGLCAKCYGRDLARGHLVNTGEAVGIIAA
                     QSIGEPGTQLTMRTFHIGGAASRATAANNIQIKTKGVIRLHNIKTVTHENKNLVAVSR
                     SGEVTIVDEFGRERERYKVPYGAVISAQDNSPVEAGQVIATWDPHTHPVISEVSGRLK
                     FVDLIDGITMNRQTDELTGLSNIVIIDAKQRSAAGRDLRPMVKLVTDEGDDIYLAGTN
                     VPAQYYLPVDAIVNFEDGSLVGIGDVIARIPQERSKTRDITGGLPRVADLFEARKPKD
                     SAVMAEVSGLVNFGKETKGKRRLIINVSEDQCHEELIPKWRHISVFEGEHVERGEIIA
                     EGALNPHDILRLLGVGALANYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKRVITFA
                     GDSKFLVGEQVEESAMLQENDKLLAEGKQIARGTPILLGITKASLATESFISAASFQE
                     TTRVLTEAAVSGKVDELRGLKENVMVGRLIPAGTGYTYHQSRKAKRARVAAGGDSSAT
                     HTVTASDVEHALSEALNADNHEH"
     gene            15066..15446
                     /gene="rpsL"
                     /locus_tag="AC06IO_001663"
     CDS             15066..15446
                     /gene="rpsL"
                     /locus_tag="AC06IO_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIO9874843.1"
                     /translation="MATINQLVRKPRVDVKKKSNVPALESCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTSGVNDRKQGRSKYGTKKPKDKK"
     gene            15467..15994
                     /gene="rpsG"
                     /locus_tag="AC06IO_001664"
     CDS             15467..15994
                     /gene="rpsG"
                     /locus_tag="AC06IO_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIO9874844.1"
                     /translation="MPRRREVPKREILPDPKHHSELLAKFINVLMVSGKKSIAEKITY
                     GALSVMEERVKKIKKNEEDGSETGSSGSAGAVLRYFEEALDNVRPSVEVRSRRVGGAT
                     YQVPVEVRHDRSIALGMRWIVQAARTRGEKGMMLRLAGELMDAYENKGSAVKKREDTH
                     KMAKANQAFAHFRWN"
     gene            16009..18093
                     /gene="fusA"
                     /locus_tag="AC06IO_001665"
     CDS             16009..18093
                     /gene="fusA"
                     /locus_tag="AC06IO_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIO9874845.1"
                     /translation="MATPLKLYRNIGIAAHVDAGKTTTTERVLYYTGMSHKIGEVHDG
                     AATMDWMVQEQERGITITSAATTCYWSGMDKQFESHRINIIDTPGHVDFMIEVERSLR
                     VLDGAVVVFDSVAGVEPQSETVWRQANKYGVPRIVFVNKMDRMGANFLRVVSQIKQRL
                     GSTPVVLQLPIGAEEEFKGVIDLIKMKAIHWDEENKGMTFKYVDIPADLKATCEEYRA
                     HIIEAAAEYSEELMEKYLEGEEFTEAEIKKALRHLTITNKVVPVFCGSAFKNKGVQAV
                     LDGVIEYLPSPTDIPDIQGVDEHGDEIHRKTSYDEPFSALAFKIATDPFVGTLTYFRA
                     YSGILKSGDTVYNSVKGKKERIGRLLQMHANSREEIKEVRAGDIAAAVGLKTVTTGDT
                     LCDQDKVVILERMDFPDPVIAVAVEPKTKADQEKMGIALGKLAQEDPSFRVHTDEESG
                     QTIIQGMGELHLEIIVDRMKREFNVEANVGKPQVAYRETLKQAIEQEGKFVRQSGGRG
                     QYGHVWLKIEPQEPGKGYEFINAIVGGVIPKEYIPAVDKGIQEQMQNGVIAGYPVVDV
                     KVTLFDGSFHEVDSSEMAFKIAGSQCFKQGALKAKPVLLEPIMSVEVVTPEDYMGDVM
                     GDLNRRRGLVQGMEDSPAGKIVRAEVPLAEMFGYSTDLRSATQGRATYTMEFCKYAEA
                     PTNIAEAIIKKQ"
BASE COUNT         5710 a         3210 c         4354 g         4838 t
ORIGIN      
        1 gccccggtaa acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg
       61 taagttccga cctgcacgaa tggcgtaacg atggccacac tgtctccacc cgagactcag
      121 tgaaattgaa atcgctgtga agatgcagtg tacccgcggc tagacggaaa gaccccgtga
      181 acctttacta tagttttgca ctggactttg atgatgactg tgtaggatag gtgggaggct
      241 gtgaagtgag gacgctagtt ctcatggagc cgcccttgaa ataccaccct gttgttattg
      301 aggttctaac ttggtccagt aatcctggat gaggacagtg tatgatgggt agtttgactg
      361 gggcggtctc ctcccaaaga gtaacggagg agcacaaagg taccctcggt acggtcggac
      421 atcgtaccaa gagtgtaaag gcaaaagggt gcttgactgc gagagtgacg gctcgagcag
      481 gaacgaaagt tggtcttagt gatccggtgg ttctgaatgg aagggccatc gctcaacgga
      541 taaaaggtac tccggggata acaggctgat accgcccaag agttcatatc gacggcggtg
      601 tttggcacct cgatgtcggc tcatcacatc ctggggctga agcaggtccc aagggtatgg
      661 ctgttcgcca tttaaagtgg tacgcgagct gggtttagaa cgtcgtgaga cagttcggtc
      721 cctatctgcc gtgggcgtag gaaaattgag aggagctgct cctagtacga gaggaccgga
      781 gtggacgaac ctctggtgca ccggttgtca cgccagtggc atggccgggt agctaagttc
      841 ggacgggata accgctgaaa gcatctaagc gggaagcctc cctcaagatg agttttccca
      901 tgaagcccgt tgaagactac gacgttgata ggcaaggtgt ggaagcgcag taatgcgtga
      961 agctaacttg tactaattgg ctgattgtct tgaccatata atctgagtga cttcagaatg
     1021 tgatattgat ttgtatacgt gaaacgtatc gtgtaaactc tgactcttta ccaaacctgt
     1081 ggcttaataa tgcaatcaaa gcctcaggta aaccagtttt cctggcgact atagcgattt
     1141 ggaaccacct gataccatct cgaactcaga agtgaaacat ttccgcgcca atgatagtgt
     1201 gaggcttcct catgcgaaag taggtcatcg ccagggtttt attttttagt gcggtatgta
     1261 aagccgacta aattaaaata ggttagttgt atacgcataa tataagttaa ataagtgcta
     1321 acctatcata tgtaaagaat atgctggcgt agctcagttg gtagagcaac tgacttgtaa
     1381 tcagtaggtc ccgggttcga ttcctggtgc cagcaccatc taaatagggc taaacgttga
     1441 ctaaaaatgt tgacaatcca ttcatcttaa aagacaatag cgttcttttt ggaggggttc
     1501 ccgagcggtc aaagggatca gactgtaaat ctgacggctc agccttcgaa ggttcgaatc
     1561 cttccccctc caccagttaa gagagaagtt agaaagcggg tgtagttcaa tggtagaact
     1621 tcagccttcc aagctgatag cgtgggttcg attcccatca cccgctccaa agtagtgaat
     1681 agtatttgct tgttggtgtg agttagcaag ctgatatgaa agcccacata gctcaggcgg
     1741 tagagcactt ccttggtaag gaagaggtcg ttggttcgag tccagttgtg ggcaccatgt
     1801 aatataaatt agcaaacggg gagactttcc gatggcgaag gaaaaatttg aacgtaagaa
     1861 gccgcacgta aacgtgggca cgattggtca cgtagaccat ggtaagacga cattgacagc
     1921 agctattaca acgattatgg cgaagaaata tggtggtaca gcgaaggcgt acgatcaaat
     1981 tgatgctgcg ccagaagaaa gagagcgtgg tattactata tctacagccc atgttgaata
     2041 cgaatcagcg agcagacatt atgcacacgt agactgcccg ggacatgctg actatgttaa
     2101 gaacatgatt actggtgctg cgcaaatgga cggagcgata ctggttgtat cagcagctga
     2161 tggtcctatg ccacaaacaa gggaacacat tctattgtct cgccaggtag gtgttccata
     2221 tattgttgtg ttcatgaaca aagcggatat ggttgatgac cctgagttat tagagttagt
     2281 ggaaatggaa gtgcgagatt tattaagcag ttacgatttc ccaggggatg atatacctat
     2341 tgtagttggt tcagctttga aagcattgga aggtgaagac agtgatatag gtgttaaggc
     2401 tattgagaaa ttagttgaaa caatggattc atacattcct gagccagtta gaaacataga
     2461 caagccattc ttgttgccga ttgaagacgt gttttcaatt tctggacgcg gaacagtggt
     2521 aactggtcgt gttgagagtg gaattgttaa agttggtgag gaagttgaaa ttgttggaat
     2581 aagagacacc caaaagacga cttgtacggg tgttgagatg ttccgtaaat tacttgatga
     2641 aggtcgagct ggtgataacg ttggtgtgtt attacgaggt acgaagcgag atgaagtgga
     2701 gcgtggacag gtattggcta agccaggaac catcaagcca cacaccaagt ttgaagcaga
     2761 agtgtatgta ttatccaagg aagaaggcgg acgtcacact ccattcttta atggataccg
     2821 tccacaattc tatttcagaa ccactgacgt gacaggtact tgtgacttgc catcaggagt
     2881 tgaaatggta atgcctggag ataatgtgca attagttgtt agcttgcatg ctccgattgc
     2941 gatggatgaa ggtttaagat tcgcaattag agagggtggc cgaactgttg gcgccggtgt
     3001 agtcgctaaa ataatcgagt aatagaaaaa agtacaaaag tggcatgagc cttatataca
     3061 tggctcatgt caataaaatt taggccagta gctcaattgg cagagtggcg gtctccaaaa
     3121 ccgcaggttg ggggttcgat tccctcctgg cctgccaact acaagtaaat atgaaaagtt
     3181 caaacgaaaa tcaaggtaag atgaaagata taatgtcctg gctagctatc atattagcta
     3241 cagcaggagc ttttgtatgt acttactatt atacgttcac aggaccaatt caatcgataa
     3301 tatggcttgc ttggcttatt ttgatccttt tcttgggtta cttaacaaca acaggtaaaa
     3361 gagtctttgt gtttgcgcaa gaagcaaaag ttgaattatt aaaagtagtt tggcctacac
     3421 ggcaagaaac aatacagaca actactatag taatggtgat ggttggattg acaggattta
     3481 tactctgggg agtagattca atcatgatgt gggcaattgc taagttaaca catttagggt
     3541 gaatatggtt gaggaaaaca aagcaaaaca gtggtacgtt gtacatgcct attcaggata
     3601 tgaaaatttt gtcatgcgcg aaataacatc tcgtgcaaaa caccataatt tacaagataa
     3661 aataggcgag gtagttgtac cttcagaaga agttgttgaa atgcgttcag gccaaaaacg
     3721 taaaagtacc cgcaaatttt tcccaggcta tgtactcgtt catatggtaa tggatgatca
     3781 aacatggcac atggtcagag gcatacccag agttctaggc tttattggag gaaccagtca
     3841 aaccccgact ccgatttctg ataaagaagc tcaggccatt atgcaacgag ttgaagatgg
     3901 cgtaactaag ccaaggccaa agattctatt tgagcctgga gaagtaatca gagtgaaaga
     3961 aggtccattt gttgacttta atggagttgt tgaagaggtc aattatgaga aaaacaggct
     4021 aagagtcgca gttcttattt ttggacgttc aactccagta gaacttgagt tcagtcaagt
     4081 agaaaaaaca taaatgattt agataagaat ttaaatttaa aggttttaca aaaccgggga
     4141 gctaggactt aattgaaaat gctaattcaa taaaagctag cgcatgaccc ataaggagta
     4201 ataatggcaa aaaaagtaga agcatatatt aaattacaga ttcctgcagg aaaagcaaat
     4261 cctagccctc cagttggacc tgctttaggt caacgtgggg taaatattat ggaattctgt
     4321 aaagcattca atgctgcgac acaacaaatg gaacaaggtc tgcctattcc agttgttatc
     4381 actgtataca gtgatcgtag ttttaccttt ataaccaaga cccctccagc ttcagttctg
     4441 ctgaaaaagg ctgctggtat tcaaagcgga agtggcacac caaatactaa aaaagtagcc
     4501 aaactgaatg ttagtcaact tgaagaaatt gctaaggtca agaaacctga tctgacagcg
     4561 gctgatctag cggcggcagt aagaagtatt gcaggtacag cacgcagcat gggtattgaa
     4621 gttgaaggtt tggaataagg ggttaccatg tcaaaattaa ctaagaaaca aaagaaaata
     4681 gcggaagtta ttaagccaaa tcaactctat acagttgctg atgctgtagc tattctaaag
     4741 caatttgcat caaaaaaatt tcgtgagagc cttgatatca gtattaattt aggagttgat
     4801 cctcgtaagt cggatcaagt agtacgatct tccactaatc tgccaaaagg tacaggaaaa
     4861 acagtacgag ttgccgtttt tgcccaaggt gacaatgctg ctaaagcaac tgctgcaggt
     4921 gccgatatag tagggtttga agatctggct gataaaatca aagcaggcga aatgaatttt
     4981 gatgttgtta ttgcaactcc agatgctatg agaatcgtag gtcagctcgg acagattttg
     5041 ggaccgaggg gactcatgcc caatcctaag gttggaacag taacaactaa cgtggaagca
     5101 gcggttaatg atgctaaatc aggacaagta agatatagaa ctgataaaaa tggaatcatt
     5161 cattgtactg ttggaaaggc agattttgct cctgaagatg ttcttgaaaa cgtagttgcg
     5221 ttaatcaatg atctgaagaa ggcaaagcca gcatcctcta aagggcaata tctgaagaaa
     5281 atatctttat ctacaacaat gggacctggg ttacctattg atatttcatc aatacctgtg
     5341 taacatatca tcccattttt aagaattcac ggcatagatt aggttccatg ccgtcgaaga
     5401 ccgcaggtgc ctaggcttaa tttcctgcgc agacggtgaa ccggctcctt aataaagaga
     5461 cggccaccat aactgtcaag tccatgagat ttgtacatat taggaggtca gtaacgtgac
     5521 attaaattta gctgcaaaaa aagccgttgt tgaagaagtg actgcagtcg cgtcaaaggc
     5581 tatttcagca gtggttgctg attatcgtgg gttaactgta aatcaaatga cgcaattgcg
     5641 tagtgaagca cggaaatccg gtgtttatct tcgtgtggtg cgaaatacac taacaagaag
     5701 agcgtttaaa aacactgaat ttgaatgctt gaatgactta cttgtaggtc cagtattcat
     5761 tgcattgtca ctggaagcac caagtgatgc tgcaaggcta ctgaaagatt acgcaaaaac
     5821 atttgaaaaa cttgagatta gagcattatc agttggcggg aaggtataca acgcaaatca
     5881 aattgatgct gttgcgagct tgccaacacg cgatgaagca atatccaagt taatgtatgt
     5941 gatgaaagcg ccaattgaga aatttgttcg cacacttgca gagccacatg ccaagttggc
     6001 tagaactctt gctgctgtaa aggacaaaaa agcaggcaat cccgcttaat cattatttaa
     6061 ttttaattag gagctagtaa tggctgtatc aaaaaacgaa attttagaaa caatttctaa
     6121 catgacagta atggaagttg ttgaattaat cgaggcaatg gaagaaaaat ttaatgtttc
     6181 tgctgccgct gctgccgtag ctgttgcagc gcctgctgca ggtgctggtg ccgctgctgc
     6241 tgaagaacaa actgagttta ctgttgtaat gaccagcttt ggttctaaca aggtaaacgt
     6301 aatcaaggca attcgtggca taactggtct gggattgaaa gaagctaaag acttagtaga
     6361 aggtgcacca tcaactgtta aagaaggtgt atcaaaagat gaagctgcca gtatcaagaa
     6421 agagcttgaa gaagctggtg ctacagtaga agttaaataa ttaaaatata gcagtattta
     6481 agaacccagc catgttcaca tggctgggtt tactcgtttg aaaatcatca ataatttaca
     6541 gaggaaccac catggcagtt gcagaagcta aacctcaata ttcgcatgct gagaaaaaac
     6601 gatttcgcaa aagctttggt aagcagacag atataatgcc aataccgaat ctgcttgaaa
     6661 ttcaacttaa atcctacagg gattttcttc aaactgatac taaattaagc gaacaactga
     6721 atacgggatt gcatgctgca ttttcttctg tatttcctat cgaaagcttt tctggtaatg
     6781 caagactgga atacgtcgga tacaagcttg gagagcctgc atttgatgtt cgcgaatgca
     6841 aactacgagg gctaacttac tcagctcctc taagagtcaa gatcagactc gttgtgcttg
     6901 ataaagatgc aagcgatgat cctaagccta taaaggatat tagagagcaa gatgttttta
     6961 tgggggaaat tcccctaatg actgacgttg gtacgtttgt ggtcaatgga actgaacgtg
     7021 ttgttgtttc acaattacat cgttcgcctg gagttatatt tgaacatgac aagggaaaaa
     7081 ctcactcttc aggtaaattg ctctattctg cacgaataat accttatcgt ggttcatggt
     7141 tagactttga attcgatcct aaagattgcg tatatgtacg tattgacaga cgtcgcaaac
     7201 tgccagtgac cattttgctc agagcattgg gctacgaagc agaagatata ttaaacgaat
     7261 tttttgaaac aacttgttgc catttaaaaa atggcgaata ccacatcgat ttaattccac
     7321 agcgtttgcg tggtgaaatc gcttcattcg atattcatgt ccctgagact ggagagctta
     7381 tcgtagagca aggtcgtcga attaccgcaa gacatataaa gcaaatggaa aaaagccaaa
     7441 tgcaggatct agtggtacca agagactatt taattggaaa aacattagca aaaaatatta
     7501 ttgatacctc aaccggtgaa tttctggctc aagcgaatga tgagataacg gaagagcttt
     7561 tagatgctat ggctagccat ggtattttac aaattgatat gatctatact aacgatttgg
     7621 atcatggttc ttacatatcg gatacattaa aaattgatcc cacaggatca caactagaag
     7681 cgctcgttga aatttaccgc atgatgcgcc ctggagagcc tcctacaaag gaagctgctg
     7741 aggcactatt taaaaatcta ttctttgttg aagaaagata tgatctatct gctgttggca
     7801 gaatgaaatt taatcgtcgg gtaggaatta agagtgacga aggcccaggc actttaacga
     7861 aggaagacat tttatccgtc attaaaacac tgatagatat tcgaaatgga atagggatgg
     7921 ttgatgatat cgatcatcta ggaaatcgta gggtaagaag tgttggtgaa atgaccgaaa
     7981 atcagtttag agtaggtctt gttcgtgttg aacgtgctgt taaagagcgt ttaagtttgg
     8041 ttgaatctga aaatctgatg ccacaggact taattaacgc aaaaccggtg tccgccgcaa
     8101 ttaaagagtt ttttggatcc agtcagttat cacagtttat ggatcaagtg aatccattat
     8161 ctggtgttac gcataaaaga cgtgtatcgg ctcttgggcc aggtggttta acacgtgagc
     8221 gtgctggatt tgaagtccgt gacgtacata caacacacta tggtcgtgtg tgcccaattg
     8281 aaacacctga aggaccaaat attggtctaa tcaattcact gtcagtttat gcaagaacta
     8341 acgaatatgg atttattgaa actccttgtc gaaaggtcgt taatgggcgt gtgacagatg
     8401 aagttgaata cctatcggct attgaggaag ttgatcaata cattgcacaa tctaatgttg
     8461 aattagacgc acaaggaaat attcttgctg acttagttcc ttgcagacat caaaatgaat
     8521 tttcactgac tactccggat aaaattaact acatggatgt atcgcccaag caaattgtgt
     8581 cagttgctgc ttcattgatt ccgtttttag agcacgatga tgcaaacaga gcattaatgg
     8641 gttcaaacat gcaacgacag gctgtgccaa ccttgcgttc agaaaagccc ttggttggga
     8701 ccggtatgga aagaatcgtt gcatctgatt caggagtttc agttgttgcg aagagaggtg
     8761 gagttattga tctggttgat gcttctcgta ttgttgttcg agtgaatgac gacgaaacaa
     8821 cagcagggga aacaggggta gatatttata acttaaccaa gtatttccgt tctaaccagg
     8881 atacttgtat caatcaaaga cccatagtat caacaggtga tcgaatacaa cgaggtgatg
     8941 tcctggctga tggtccatgt acagatatgg gtgaattggc tcttggacag aatctactgg
     9001 tagcgtttat gccttggaat ggttataact ttgaggattc cattcttata tctgaacgaa
     9061 ttgttcatga cgacagattc actactattc acatcgaaga gctaacatgt atagctcgag
     9121 ataccaaatt aggtacggaa gaaattacag cagatattcc caatgtaggc gagtctgcac
     9181 tctctaattt agatgaatct ggagttgttt acataggtgc agaagttaaa gcaggtgata
     9241 ttcttgtggg gaaagtaact cctaaaggag aaacccaact aactccagaa gaaaaactgt
     9301 taagagccat ctttggtgaa aaagcttcag atgttaaaga ttcatctttg agagttccat
     9361 caggtatgaa tggaacagtg atcgatgtac aagtctttac ccgtgatgga cttgaaaagg
     9421 atgctcgtgc taaaagcatt gaagaagagc atctggctcg agttcgcaag gatttaatag
     9481 acgaacggcg tattcgtgaa gaagacatct atcatagagt ttctcatttg ctgttggata
     9541 aggttgcaac aggcggtcct ggaagcctaa agcctggatc taaaatcacc caggattacc
     9601 tagataaagt tgaaagagaa aagtggtttg atattcgtat agaagatgac gcgattagtc
     9661 agcaacttga acaactatcc aagcaactgg aacttttaac aaaagaaatg gaaaagcgct
     9721 ttaacgacag tagaaagaaa attatccagg gagacgattt agctcctggt gtattaaaaa
     9781 ttgttaaagt gtatttggct gttaaaagaa gaattcagcc tggagacaaa atggcaggtc
     9841 gtcatggaaa caagggtgtg atctccattg ttgttccagt tgaagatatg ccacatatgg
     9901 aagatggaac ggctgtagat atcgttctta atccattggg cgtaccatcg cgaatgaata
     9961 tcggccaggt tcttgaaact catctgggac tagctgccaa aggattggga agaaagattg
    10021 cacagatgct tgatgaaaag caaacacctg aagcaataaa agcatacctt gagaaaattt
    10081 ataatcatga cggagtgcag cgtgttaatt tgaaatgcct caatgatgat gaattgatga
    10141 cactggcgga taacctgaga gctggtgtgc cgatggcaac tcccgtcttt gatggtgcta
    10201 ctgagcaaga aatcaaaagt atgctgcagt tagcagattt gccagcagat ggtaaaaccg
    10261 tgttgataga tggccgaaca ggtaataaat ttgataatcc ggttactgta ggctacatgt
    10321 atatgttgaa gttaaaccat ttggttgatg acaagatgca tgctcgttca actggatcat
    10381 acagtttggt cactcaacaa ccacttggcg gtaaagcaca gtttggtggt cagcgttttg
    10441 gtgagatgga agtatgggca ttagaagcat atggtgcagc atacacttta caagaaatgt
    10501 taaccgtaaa atcagatgac gtcggaggtc gaacaaagat ctataaaaat atagtcgatg
    10561 gagatcatcg tatggaccca ggaatgcctg aatccttcaa tgtattattg aaggaaatta
    10621 gagcactggg aattgatatc gagttggagc atgactaact catcagcgat acatactgac
    10681 taaccatttt ttggaggcat agacttggct accctaagca acgacccaat gaaaaaagca
    10741 ccagtaatga gtgacttact aggtattctt aagcaacaag gtcaaagtga agaatttgat
    10801 gcaataaaaa ttgcactggc gtcacctgaa ttaattcgat catggtccta cggcgaagtt
    10861 aaaaaacctg aaacaatcaa ctacaggaca tttaagcctg aaagagatgg tttgttttgt
    10921 gctaaaactt ttggtccagt taaagattat gagtgtttgt gtggaaagta caagcgacta
    10981 aagcatcgtg gtgttatttg cgagaagtgt ggggttgaat tggctcttgc caaagtgaga
    11041 cgagaaagga tggggcatat cgaactcgct agcccagtcg cacacatatg gttcttaaaa
    11101 tccttaccat caagaattgg tttgctcctc gatatgacac taagagatat cgagagagta
    11161 ctctattttg aggcttttgt tgttgttgat cctggaatga cagaacttga aagagggcaa
    11221 ctacttaacg acgaagccta tcttgatgcg atggagcagt atggagatga gtttgacgcg
    11281 cgtatgggcg cagaagcaat tcgtgattta ttgcgccaaa ttgatctgga agatgaaatt
    11341 agaaatttac gtgaagagct tccaacaact aactctgaga cgaagattaa aaaaatcacc
    11401 aaaagactca agttattaga agctttttac gagtcaggaa ataaaccaga atggatgatc
    11461 atggatgtac ttccagtgct tccacccgat ttgagaccat tggttccgct agatggtgga
    11521 cgttttgcta cttcagactt gaatgattta tacaggcgcg tcattaatag gaataaccga
    11581 ctaaaacgcc tgctggatct gaatgcgcct gacattatcg ttcgtaacga aaaacgaatg
    11641 ctgcaagaat ccgtagatgc tttgcttgat aacggcagaa ggggtcgtgc catcactggt
    11701 accaataaga gacctcttaa atcactggct gatatgatta aaggtaaaca gggtcgtttc
    11761 aggcaaaacc tcttaggtaa gcgtgtcgac tactctggac gttcagttat tgtagttgga
    11821 ccaactttaa aattgcacca atgcggattg cctaaaaaaa tggcgttgga attattcaag
    11881 ccatttatat tttccaaatt ggaatttaga ggtttggcaa ctactatcaa agcagccaag
    11941 aaaatggtcg aaagagaaga atcggttgtt tgggatatat tagatgatgt tattagggaa
    12001 catcctatcc ttttaaaccg tgctcctact ttgcatagat taggtattca agcatttgag
    12061 cctgttttaa ttgaaggtaa agcgatccag ctccatcctt tggtatgtac agcatacaac
    12121 gctgacttcg atggagacca aatggctgtg cacgtgccct tgacactgga agcgcagctg
    12181 gaagctcgtt cattgatgat gtctacaaac aatatattat ctccagccag tggtgagccg
    12241 attattgttc ccagtcagga cgtcgtcctt gggttatact atttaacacg agaaaaggtt
    12301 aatgcattag gcgaaggaaa aatatattca agtgctcagg aagcgcaaaa tttctatgaa
    12361 gcaggtcatt tggatatcca tgcaaaaatt aaaattcgca tgcctaaaga agatggtgaa
    12421 actggatatc atttagttga gacaacagtc ggacgcgcta ttttagctga gattttgcct
    12481 aaaggaatgc catttgacta tataaaccgt acaatgacaa aaaaggttat ctcaaaagta
    12541 atagacagtt gctaccgcaa attcggcttg aaagaaacag tcatttttgc tgatcagtta
    12601 atgtataccg gatttaaata cgccacacga tctggagcat ccataggtat tgaagacatg
    12661 gaaataccag atgataaggc cagtattatt gaacacgccg ataatgaagt aagagaaatc
    12721 gaatctcaat tcagatcggg tttggtaacg aatggtgaac gatacaacaa ggtaattgat
    12781 atttggtcaa gaactaatga attagttgcc aaatcaatga tgtctaaaat tgcaacagaa
    12841 gaagttactg atgcaaaagg caacaaagta agacaagaat catttaaccc aattttcatg
    12901 atggctgatt ctggagctag aggttctgct gctcagatac gacagcttgc cgggatgcga
    12961 ggtttgatgg cagctccaga tggctcaatt attgaaacgc ctatcactgc aaacttccgc
    13021 gaaggtttga atgtattcca gtactttatt tctacacacg gagctcgtaa gggtttggca
    13081 gatacggcgt taaagacagc aaactcaggg tatctgacac gacgcttggt agatgttgcg
    13141 caggatgttg ttattaccga agatgattgt ggtactgata ctggtattct aatgcaacca
    13201 ctcattgaag gtggtgatat tgttgagcct ttgcatgaac gcgtccttgg gcgggtggtt
    13261 gcttcagatg tttatattcc tactcaaact gaaccagtag tcaaggctgg aactttactg
    13321 gatgaagaat gggttgaaaa attagagaag catggggtag accaggttat ggtcaggtca
    13381 ccaatcacct gtcaaacaag atttggtctt tgcgcaaaat gctatggtag agatcttgct
    13441 cgtgggcatc ttgtcaacac aggtgaggca gttggtatta tcgcagcaca atcaatcggt
    13501 gagccaggaa cacagttaac catgcgtacc ttccacattg gaggtgctgc atcaagggca
    13561 acagctgcga ataatattca aattaaaacg aaaggggtta ttcggttaca taatattaag
    13621 accgtaactc atgagaataa aaacctggtg gcagtttctc gctcaggtga ggtaactatt
    13681 gttgatgagt tcggacgaga aagggagcga tataaagttc cttatggtgc agtaatttca
    13741 gcacaggata attctcctgt tgaagctggt caggttattg caacgtggga tcctcatacg
    13801 catcctgtta tttccgaagt gtcagggcgt ttgaagtttg ttgatctcat tgatggtatt
    13861 acaatgaata ggcaaactga tgaattaacc ggattaagta atattgtaat aattgatgcc
    13921 aaacaacgga gtgctgcagg tcgagatttg cgcccaatgg taaaattagt tacagatgaa
    13981 ggggatgaca tatatcttgc tggaaccaat gttccggcac aatattatct acctgtcgat
    14041 gccattgtga actttgaaga tggaagtctg gtgggaatag gagatgttat tgctcgtatt
    14101 ccgcaagaac gttctaagac tcgtgatatt accggaggtt tgccaagagt agcggactta
    14161 tttgaagcca gaaaaccaaa ggattctgca gtaatggcag aagtatcagg attggttaat
    14221 tttggtaaag aaaccaaagg taagcgacgc ttgattatca acgtatcaga agatcaatgt
    14281 catgaagagc ttattcctaa atggcgtcat atttcagtgt ttgaaggcga gcacgtagag
    14341 cgtggtgaaa tcatcgcaga gggtgcactt aaccctcatg atatattgcg cttgttaggt
    14401 gttggagcgc tggctaatta catcgtaaat gaggtccagg acgtctatcg tttacaaggt
    14461 gtgaaaatta atgataaaca cattgaagta attgtcagac aaatgctgcg taagagagtt
    14521 attactttcg ctggtgactc gaaattcctc gtaggagagc aagtagaaga gagcgctatg
    14581 cttcaggaaa atgataaact tctggctgaa ggaaagcaga ttgcgcgagg tacacctatt
    14641 ttactaggta taacaaaagc ttcattagct acagaatcgt ttatatcagc cgcttcattc
    14701 caagagacaa ccagagtctt gactgaagca gcagtaagcg gaaaagttga tgaattgcgt
    14761 ggtttaaaag aaaacgtgat ggtaggtcgc ctaattccag caggaacagg ttatacttat
    14821 catcaaagcc gaaaggcaaa aagagcaagg gttgcagcag gcggagacag ttcagcaaca
    14881 cacacagtta ctgcaagtga tgttgaacac gcgttgagtg aagcattaaa cgcggataat
    14941 catgaacatt aatcaggatt caaaaatggc agatttaagc ttgacaaatc tgccgtacct
    15001 atatagaatc cggcctcctt atgcattcaa aatattcaca agtgatagat cggagttaag
    15061 tacaaatggc tactattaat cagttagtaa gaaagcctcg tgtggacgta aagaagaaaa
    15121 gcaacgtgcc agcacttgag tcatgtccac aaagacgcgg tgtatgtact cgtgtataca
    15181 ctactacacc aaaaaaacct aactcagcta tgcgtaaggt tgcacgtgtg cgcttgacca
    15241 atggatttga agtaacatcc tatattggtg gtgagggtca taatttacaa gagcactctg
    15301 ttgttttaat tagaggtggt cgtgttaagg atttacctgg tgtgcgctat catacagttc
    15361 gaggtagctt agatacttca ggagtgaacg accgaaaaca agggcgttcc aagtatggta
    15421 caaaaaagcc aaaagataaa aaataaccga ttgtaggaaa attgagatgc caagaagacg
    15481 agaagtaccc aaacgagaaa ttttacctga tccaaaacat catagtgaac tgcttgctaa
    15541 gttcatcaac gttttaatgg ttagtggtaa aaaatcaatt gctgaaaaaa tcacttatgg
    15601 tgcgctgtct gttatggaag agcgtgtcaa aaaaattaag aaaaatgagg aagatggaag
    15661 tgaaactggc agcagcggaa gtgccggtgc cgtacttcgt tattttgaag aggctttaga
    15721 taacgttcgc ccaagtgtgg aagtacgttc acgccgagtt ggtggcgcaa cataccaagt
    15781 tccagtagaa gttcgacatg atagaagtat agcgcttgga atgcggtgga tagttcaagc
    15841 tgctcgcaca cgtggtgaaa aaggtatgat gctgcgtttg gcaggggaac tgatggacgc
    15901 ttatgaaaat aaagggtctg cagtaaagaa acgtgaagat acacataaaa tggcaaaagc
    15961 taaccaagcc tttgctcatt ttagatggaa ttaagagaga gataagccgt ggcaactcca
    16021 ttaaaactat atagaaacat aggcattgca gcccacgttg atgctggaaa aacaacaact
    16081 acagaacgag tattgtacta taccggaatg tctcacaaga ttggtgaggt acatgatggt
    16141 gctgcaacta tggactggat ggtccaggag caggaacgtg gtattaccat tacttctgct
    16201 gcaacaactt gttattggtc tggtatggac aaacagtttg aatcccatcg gatcaatata
    16261 attgatacac caggacacgt agactttatg attgaagtcg aacgctcttt acgtgtacta
    16321 gatggagcag ttgttgtatt tgattcagtt gccggcgttg aacctcaatc tgaaacagta
    16381 tggcgtcaag ccaataaata tggtgttcct cgtatcgttt ttgtaaataa aatggatagg
    16441 atgggtgcca atttcctaag agttgttagc cagattaagc aacgattagg ctctactcct
    16501 gttgtgttac aattaccaat tggtgcagaa gaagagttca aaggtgtgat tgatctcatt
    16561 aaaatgaagg caattcactg ggatgaagaa aataaaggta tgacatttaa atatgttgat
    16621 atccctgctg atttaaaggc aacctgtgaa gaataccgtg cacacatcat tgaagcagca
    16681 gcagaatatt ctgaagagtt aatggaaaaa tatttggaag gtgaagaatt cacagaggcg
    16741 gaaattaaaa aggctcttcg ccatctgaca atcacaaata aagttgttcc tgtattctgt
    16801 gggtcagcct ttaagaataa gggcgttcaa gcagtgctcg atggggtaat cgaatatttg
    16861 ccatcaccca ctgacattcc tgatattcag ggagttgatg agcatggtga tgaaatccat
    16921 agaaaaacca gctatgatga gccattctca gcgttagcgt tcaaaattgc gactgaccca
    16981 tttgtgggca cactaacata ttttagagct tattctggta ttcttaagag tggggataca
    17041 gtatataact cagttaaggg caaaaaggaa cgtatcggcc gcttgttgca gatgcatgct
    17101 aattctcgtg aagaaattaa agaagtgcgt gcaggggata tagctgctgc tgttggtctt
    17161 aaaacagtaa ccactggtga taccctgtgt gatcaagata aagttgtaat attagagaga
    17221 atggatttcc ctgatccagt tattgcagtt gctgttgaac caaaaactaa agcggatcag
    17281 gaaaaaatgg gaattgcttt aggcaagtta gcccaggaag acccttcttt cagagtacat
    17341 actgatgaag agtcaggtca gacaatcatt caaggtatgg gtgaactgca ccttgaaatt
    17401 attgttgacc gcatgaagag agagttcaat gtagaagcta atgttggtaa acctcaggtt
    17461 gcataccgag aaacactcaa gcaagctatt gaacaagagg gtaagttcgt aagacaatca
    17521 ggtggacgtg gtcaatatgg acatgtatgg ttgaaaattg aaccacaaga accaggaaaa
    17581 ggttatgaat ttatcaatgc tatcgttggt ggcgtcattc caaaagaata tatccctgca
    17641 gtagataaag ggattcaaga acaaatgcaa aatggtgtaa ttgctggata tccagtggtt
    17701 gatgttaaag ttacactgtt tgatggttca ttccatgaag ttgattcaag cgaaatggca
    17761 tttaaaattg ctgggtctca atgcttcaag caaggtgcac taaaggcgaa accggttctt
    17821 cttgagccaa ttatgagtgt cgaagttgtt acacccgaag attacatggg cgatgttatg
    17881 ggtgatctga acagacgccg tggtttagtt caaggtatgg aagactcgcc agcaggcaaa
    17941 atagttagag cagaggtgcc acttgcagag atgtttggtt attcaactga cttacgttct
    18001 gctacacaag gaagagcaac ctacactatg gagttttgta agtacgcaga agcaccaaca
    18061 aatattgctg aagcaattat caagaaacaa taaaagttaa cgaggttatt ga
//
LOCUS       DBNCWK010000057        18080 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.057, whole genome shotgun sequence.
ACCESSION   DBNCWK010000057 DBNCWK010000000
VERSION     DBNCWK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18080
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.057"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            26..3379
                     /gene="sdbA"
                     /locus_tag="AC06IO_001666"
     CDS             26..3379
                     /gene="sdbA"
                     /locus_tag="AC06IO_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbA"
                     /protein_id="HIO9874846.1"
                     /translation="MHKKYNYYSLEKEKKTFWQHILDILKAPFRLPGWVVSFFLARNI
                     THVALNPNNIPQQRLIHLTKTSNNPEDDIVVINFKKRPPHKWFNDTLIKIANTIAALP
                     FVTPRLRTRLHYDNENDINHVNKLLAEIDALVQGKSKQKYCKGRAFDWSKIHLKGLEF
                     LDPKMRGYVYEQLHEKYGYVSYTTKRKPNIEFFTLKTPDGSELDSVQVTGEDEEKKPM
                     GERKFIITCIARDQNFINWIKDLNYTAKNLGATAISFNYRGVDYSRGLVWTENNLVDD
                     ILAQVQRLISLGADPKNICLDGMCIGGAVATIAAAKLHEKGMKVKLNNERSFTSLSSL
                     VFGFIVPELQTANWWSPLTYGRFLLAGVVYALLTPLIWLAGWPVDVTKAWNRIPAQDK
                     MYSVVRDKDNGLYDGVIHDHFCSIASLVDSQINSILYKLSTDQPLTEEEKQILCDDQF
                     SHHFKPSQSVLKNPKYKGPHFISRQDLVAELGHREEYTNHDYFLDRLREKFQLDRATR
                     PVALAEDREKDIDGILSQLSNHKERPLIIASSGGTGHISATHGIINDLQSKTDNVVIT
                     QHHAELYKNKPFSITSVLIRIGVWFTSLPILEDILKGVMRFIGYPVLPSSSIFWDQMS
                     KIQQSETKKENGIETGRTRPYVDMLLDIYPEGYEYTAFNNATHLTSSIEDIQTMISFK
                     GHVEEDNRNIVYQNILQRLMHAAKQNTPYTRLISTQALSLGAICDAVKYYNTVFLPVY
                     NAERGTSYQPIAIAQYMTDLPSLGCIHFMNNLEELTSEQRQLMEIHAVNMSEPFKEAH
                     FGKEQGFKAVHNIDPRNNPMIRNAFKDPSLTKYLDKTQAFDLHFNVYKKEKQSALPVL
                     NGKEKITIKPHAKIASIMIGSLAANASADYAKYLLNQGYEHIFLFGGLNDSIAARIDQ
                     IINSYPASRRDEIRKKIILLGNQSDVEMAPIMTRSNCVVIRGGGLSVMEQMAMPIMDD
                     KIVLLHHEDNEEGPLTSGLSWEDGNADKLIEYLSEKGAYAKKTSPGLCSGHLHEAEKD
                     FEKKYITQLKSTGTKGKVDLTIPQQKKRPLKKEWDRKTGYTESGHILSHQHRFFNTIP
                     EGREPFGSKEEIHHHEQLSSQNLVSVTAG"
     gene            3622..5061
                     /gene="legG2"
                     /locus_tag="AC06IO_001667"
     CDS             3622..5061
                     /gene="legG2"
                     /locus_tag="AC06IO_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegG2"
                     /protein_id="HIO9874847.1"
                     /translation="MPKILDSNAYREMENELNNLFEQIYKHGKNDRTAITLQAQQFLD
                     NLSESIRDHSRLLLTKIAREELIYFKEKKPGDSREKFQNIDKMSDYFNNLSALINFSV
                     MQQEDPTLRVFYYDMYIQLMNFCYLKGDLFGAGAIYTGLISNNLPNSIDWKQLSSESR
                     LIFKDCEEKFKRLFNLSTTYNEHTNLKRQFKTALIPALPALLTVQIYTKDAYDNSVSD
                     FERIQEKLRQLDIEHNRFLNQLQNDHSTWSKAYYDYMSNVYLPQSIFEYTTLLREYEE
                     LVTTHGGPIAKQFHKDKNSEPLVNFVESTLSENQSVLKVSPLQNERIVEMLSEIDVVK
                     SECSSPAVFDDLSWKLRAMNCTLVKAKSIGDYTSKIFANLDTPYMEENTDEDPKPVLV
                     EELQTDDVYQLHHSGMKLMRELEQAIEDHKPKITYHCSGEANYGFFSEARRRGRHLSP
                     EPMDIEVMDVDVMDYDENDLRDQTVMITV"
     gene            complement(5127..7349)
                     /gene="narR"
                     /locus_tag="AC06IO_001668"
     CDS             complement(5127..7349)
                     /gene="narR"
                     /locus_tag="AC06IO_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosP-associated response regulator NarR"
                     /protein_id="HIO9874848.1"
                     /translation="MSTESFRILVIDDNPAIHQDFMKILNVSKNSVLLNKFDAELFDD
                     ETEQLGISLPEFEIDVATQGIEGIEKVKRSLEEGRPYPLAFVDIRMPPGIDGIETIKR
                     IWAIDPQIQIVICSAYSDYSWESTVNTLGLSDNLLVLKKPFDVIAVRQLASALTQKWV
                     LARETQKHTEFLNQLVEERTESLQQSFSLLRATIESSSDGILVVDLQGKIVDYNSQFV
                     KLWNIPESIMKKTSESQLIQYMSTQLLNSEQHAQHVKEFSIHIDDSSIQIYHFRNGKV
                     VECCSKPHRVGPQTVGRVWSFRDITEQTRLKEKLEYQATHDALTNLPNRLLLIDRIEH
                     AISSYARHKMKFAILFFDLDRFKMINDSLSHEAGDQLLCAVAKRLRSLVRKEDTLARL
                     GGDEFVMLFQSFNSEEQIAGVAQKILKSFQKPFHIAERDINIHVSIGISVYPTDGKTV
                     NTLLSNADMAMYQAKFRGGNQFSFYTEKLNKKTNQQFQLELDLQKAIIHHEFFLLYQP
                     QFTVNTHEIRSMEALIRWNHPQKGIILPLDFIPTAESSGLIVPIGEWVLRETCKQIGT
                     WRTMGLPEIRVACNVASKQLRQRDFPAMVEDLLKEYSVEPHCLELEITENVIIDKEIQ
                     RTIKKLKSLGVLIILDDFGTGNSSLNLLKQVAVDSLKIDQSFIQNISKSPGDEAIIDA
                     IIAIAQSMNFNIIAEGVETQNQLKFLKKRRCNDIQGFLMSKPIPPEEIEKLLQGNRTK
                     "
     gene            complement(7352..8767)
                     /gene="nahK"
                     /locus_tag="AC06IO_001669"
     CDS             complement(7352..8767)
                     /gene="nahK"
                     /locus_tag="AC06IO_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosP-associated histidine kinase NahK"
                     /protein_id="HIO9874849.1"
                     /translation="MTEMHRLLQRQLKKLGFTADGLPQNLESWTQFIAHINKTYYEND
                     QDRYLLERSIDISSKEMRDINSKLEYAQDIAQMGYWHYDADTDTATWSKGLFKILGLP
                     PSNVALNYSSFLDLVHPNDKAGLKNVVEKALKEKEDYDYELRVRNGDNDYKWYRTIAR
                     ANPHKNEISGVLMDISKRKIIEEQVHELNNKLVSTARLAGMSEVATSILHNVGNILNS
                     VNVSAIILKKNISNIHYKKLSAIKTMLDENINQLSEYLLQNPKGKLILPYLDALSKTL
                     HEEYEENMKEANSLETNINHIKEIIAMQQSLSGLSNLTEKISIHDVILNAVKSVILDK
                     HQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANDSRKIVISIGRTEK
                     NHIMIKINDNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNG
                     NGKGAQFVLDLPINPKTLSTR"
     gene            complement(8790..9953)
                     /gene="nosP"
                     /locus_tag="AC06IO_001670"
     CDS             complement(8790..9953)
                     /gene="nosP"
                     /locus_tag="AC06IO_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing protein NosP"
                     /protein_id="HIO9874850.1"
                     /translation="MKIESFQYIQNQGWSIPTFPELDSENTLILVFASPIFRANSKPI
                     EELAQAYKKSKIIGCSTAGEIFGPNIFDNSISVAVVQFESTKLQLAKTEVKSTEESYE
                     AGIHLSKSLHQKQNPQSIFVLSDGLNVNGSELIKGLNQHAGKGVIITGGLAGDGADFK
                     NTWIIYDGKIIDHCIGAVGFYGDQIHVGHGSRGGWDIFGPERVITRSKANILYELDNQ
                     PALKLYKTYLGDRASELPAAGLLYPLAIRNPSAPDSQHIVRTILGVNEEEQSLIFAGD
                     LPKGYYAQLMRANFDRLIVSANEAGENANDRLFKNGNLNTQPVLAIDISCVGRRLVLG
                     ELTEEETETTLQSLPPKSIQIGFYSYGELSPLATGTCELHNQTMTITTLLESP"
     gene            complement(10069..10959)
                     /locus_tag="AC06IO_001671"
     CDS             complement(10069..10959)
                     /locus_tag="AC06IO_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIO9874851.1"
                     /translation="MDIRDIKSFLAVIEYHSLTLAAKKLHVTQSAMSKRIRKIEDELN
                     VRLIISEGSKLIFTEAAKQLIPYARQMFSAHQNMVHTLKNSQQFTQSIIIGASVYVSH
                     YVLPSFFKYLKEINPAIQAHLKTISEKEVEYILNHGTVDLVICPAREVSEKLLGDSLL
                     WEEKLNLVVAANHELAHRISALSLSELSLFPAILTEKGAALRDKIDNLFEKNQLVLNL
                     GFEMSTIDAIKSIVEYGLGWSYLPDSLLSEKLKILTIKDLELTIPFHAYYLKKRINDR
                     LINDLLSNFKKWINRDDKQE"
     gene            11274..13313
                     /locus_tag="AC06IO_001672"
     CDS             11274..13313
                     /locus_tag="AC06IO_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874852.1"
                     /translation="MTKKHLNPDSQTDLGDTEKVELEDTDTVDIPTEGDLSPSKMRFI
                     WEGDFSSYQLKAADLRPITRHYKQKNHIQIVINGEHPFGQELISKKLAAMDEQIKGLN
                     DDSLKYALYIQKHWLQPGYLGDQFCISNMFGSDFSKVYKELKQLLAPYLTNDGEIKKG
                     LSTQELLDLNEIVKAKFAALFDAKITRYIAFLDESKSDVCLDKQEVIDEIKKIQSGLK
                     PGQLAGYVYTNNRRIGESHFEVVIIGPETIIKPVHWGNQGRESGLYSEDLTGMFDTKI
                     DSFVQTTSAIPLPQAGHEECGTLGIMYLNELLKDNAKQLQEDSLRFSFYDKGNSLAHF
                     FFPSPQVLRFSQSSAYNSVIKAMLEDAPDVEVIHGNKKYRVKTLRGILEESIEAAKKC
                     GNSELAARNQKILDDLPSFRKHWLQSYDLCEQKRSLMEDRHNLYLAYTTQRMRRKADE
                     HAGLQVDFELENPPLLPETHAETETETETETEIEIEIEIEATNPTSDEVMQSSSNREH
                     SFTPQQSTIIIEAMQKALTSMGTSRFVSGRDDKEKLLVQFKSAFENATNGQERHEALR
                     NFVTTASTPRQGVLNFFPAAYGKTRSATAFYEHVKNQDPSLVEVLKTMDGEKGRKVVA
                     QSNSSDETKATMPADQARGIMQHFKRAIQVERGSELKTGNDSVDSQNLCDTGLKI"
     gene            complement(13324..14508)
                     /locus_tag="AC06IO_001673"
     CDS             complement(13324..14508)
                     /locus_tag="AC06IO_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIO9874853.1"
                     /translation="MHLKRTLSLTLLAFYGLGTILGAGIYALVGEVAKEAAQFTPLSF
                     LIASIIAFFTAISYAELSSRLPLSAGSALYVRRAFQQRWLSGLIGWIVVLTGVISAAT
                     LSHGFVKYFQLFLPLPPSLIITILVVILAGVAVWGIRESATLILFMTLMEVGGLLMII
                     YYGRYSLASIDIQSFSFPHSFDGVLIGAFIAFYAFIGFEDMVNTAEETINPEKTLPKA
                     IFLALISATILYVAVAWVIVSSIPSNALVKTDMPLIEIIKLQGQSPLLFSIIALISIT
                     NGILVQIIMASRLIYGMANQQNAPQIFSFVYSKTQTPVNSTLLVSLIILLFAYALPIT
                     TLAKLTSSIILCIFIIIHASLIKIKLTEKKPPEAISFPIVFPMISILLTLTFLGIQFF
                     LK"
     gene            complement(14576..15352)
                     /locus_tag="AC06IO_001674"
     CDS             complement(14576..15352)
                     /locus_tag="AC06IO_001674"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9874854.1"
                     /translation="MSRREWPANDYAIGSYIQATVAEEYLSYLKVNPTDKILDIGCGN
                     GAFTKNILMKVPQGSVLGIDASENMLHLAQDVSKEYPNFSVQKADVLTMDFHLQFDYV
                     VSFWCLQWACANIQKAFLNIVNALKPGGKFLTLFPAGDDPFIMSYYALKKSGQFASLH
                     DFIPPVDYSHLNNLEKKLESLSCQELKVKLCRQSITLPSLDVFRKFVNGIAFYQGQLP
                     NEEIQKINEAMVQYYDNECQAKYHGEYQFNFTIYLVTGEK"
     gene            15560..16771
                     /locus_tag="AC06IO_001675"
     CDS             15560..16771
                     /locus_tag="AC06IO_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HIO9874855.1"
                     /translation="MFNQSQQKIVCVLYDDPKGGFPPNYARESIPELKQYPDGQSLPT
                     PDSIDFIPGEMLGSVSGELGLRQFLENNGHQFIVTSDKDGPDSVFARELKDATVVISQ
                     PFWPAYLTRDRIERAPKLKLAITAGIGSDHVDLQAAMEHNITVCEVTYCNSISVAEHT
                     VMMILALVRDFIPQYNMVIDGGWNIADCVSRSYDLEGMQVGCVAAGRIGLAVLRRLKP
                     FAVKLHYTDRHRLPVQLEQELNLTYHPSVESMVKVCDVVSIHCPLHPETEYLFDERLI
                     KQMRRGSYLINTARGKICDQHAVAKALESGHLAGYAGDVWFPQPPAKNHPWRSMPHHA
                     MTPHTSGTTLSAQARYAAGVREILECWLGNKPIRDEYLIVSQGRLAGVGSHSYSVGNS
                     TTGAEQEVELV"
     gene            complement(16876..18000)
                     /gene="ceg10"
                     /locus_tag="AC06IO_001676"
     CDS             complement(16876..18000)
                     /gene="ceg10"
                     /locus_tag="AC06IO_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg10"
                     /protein_id="HIO9874856.1"
                     /translation="MFTKSCAKAADKNRQELFMDSLLGDFQKMKEKALNQQQEKRSPK
                     RALNSKSSEKTKKPNQHAALTHAQVQEVKAKVRTVNDKFHLNAEEKKLWELILLGNQL
                     AQNISSCDLPTDNVDDASLVKITQIFADETLERTDLTWLNKILKIALYSRGSGFGNCQ
                     EKAFFVFALLLHQAQKPESLIHSLRLATFNNHFILIVNEQFLMDPWLNLAFPLSKGNQ
                     QLEIAYVFEGFGRLVNYFSINQEGQCFTHAVREGGTTERDPSSEKDMANCIHSLLDHR
                     DYFDLSIVSEREIHTNTKRRKYENQEQEVPQPRQETANQTTETVNIDSNHNTWDNEGA
                     ADDFLEHFEQTHPIYNPGFFSFGQDDVGLKPDLPKNLFKF"
BASE COUNT         5514 a         3306 c         3664 g         5596 t
ORIGIN      
        1 atttaattaa tttttagaag ttaatatgca taaaaaatac aactactaca gtcttgaaaa
       61 agaaaaaaaa acattctggc aacatatact ggatattctg aaggccccat ttaggctacc
      121 tgggtgggtc gtttcttttt tcttggcacg aaatattact catgtggcat taaatcctaa
      181 taatattccg caacaacgtt taatacatct tacaaaaaca agcaataatc cagaggatga
      241 tattgttgtc attaatttca aaaaacggcc tcctcacaaa tggtttaatg acacgctgat
      301 taaaatagcc aataccattg cggctcttcc ttttgtaaca ccaagattac gtacaagatt
      361 acattacgat aatgagaatg atatcaatca tgtgaacaag ttgcttgccg agatcgatgc
      421 gcttgttcag ggcaaatcaa aacaaaagta ttgcaaaggc agggcattcg attggtcaaa
      481 aattcatctg aagggattgg agttcctgga ccctaaaatg agaggctatg tttatgaaca
      541 actgcatgaa aaatatggtt atgtctcata tacaacaaaa cgcaaaccta atattgaatt
      601 tttcacctta aaaactcctg atggatctga attggattca gtgcaagtta caggtgaaga
      661 cgaagaaaaa aaaccaatgg gggagcgcaa gtttattatt acctgtattg cgcgggatca
      721 aaatttcatc aattggataa aggatttaaa ctatacggca aaaaatctgg gcgccacagc
      781 catcagcttc aactacagag gcgtggatta cagtcgcggt cttgtctgga cagaaaataa
      841 tcttgtcgac gatattctgg ctcaggtgca aaggctaatt agccttggtg ccgaccctaa
      901 gaacatctgc cttgatggta tgtgtatagg tggggcggta gcaacaattg ctgcagcaaa
      961 acttcatgag aagggcatga aagtcaagtt gaataatgaa cgctctttta cttctctttc
     1021 ttctctggta tttggtttta ttgtacctga attacaaaca gcaaattggt ggagcccttt
     1081 gacttatggc cgcttcctac tggctggtgt agtttatgct ttactcacgc ctttaatttg
     1141 gctggcagga tggccagttg atgtgaccaa agcctggaat agaatacctg ctcaggataa
     1201 aatgtattct gttgtgcggg ataaagacaa tggtttgtac gatggagtaa ttcacgatca
     1261 cttctgttcc attgcgtcgt tggttgattc tcaaattaat tctattttat ataaattatc
     1321 cactgatcag ccgcttactg aggaagaaaa acaaattttg tgtgatgatc agttttctca
     1381 ccattttaaa ccaagtcaaa gtgttttaaa aaatccaaaa tacaaaggcc cgcattttat
     1441 ttcccgacaa gatttggtag ctgagcttgg tcatcgagaa gaatatacca atcatgatta
     1501 tttccttgac cgcttgcggg aaaaatttca attggatcgc gcaacacggc cagtggcttt
     1561 ggctgaagat agggaaaagg atatagatgg gattctatcg caactatcta atcataaaga
     1621 gcgtccttta attattgcca gcagtggagg aacagggcat atctctgcca cgcatggcat
     1681 cattaatgat ttacagagta aaacagataa tgttgtcatt acgcagcatc atgcggaact
     1741 ctacaaaaac aagcccttct caattacatc tgttctgatt cgtattggtg tttggtttac
     1801 ctctcttcct atcctggaag acatactgaa aggagttatg aggtttatcg gatatcccgt
     1861 tttaccgagc tcctccatct tttgggatca aatgtctaaa atacagcaga gtgaaactaa
     1921 aaaagaaaat ggaattgaaa caggcagaac ccgtccttat gtggacatgc tgttggatat
     1981 atacccggag ggatatgaat acacggcatt caataacgcg actcacttga cttccagtat
     2041 tgaagacatt caaacgatga taagcttcaa ggggcatgtt gaagaggata acaggaatat
     2101 agtctatcaa aatatattac aacgccttat gcatgcggcg aaacagaata ctccttacac
     2161 aagattaatt tccacacagg cactttcttt gggagctatt tgcgatgccg ttaaatacta
     2221 caatacggta tttctgcctg tatacaatgc tgaaagagga acttcctatc agcctattgc
     2281 tattgctcag tatatgaccg atttgccttc actcggttgt attcatttta tgaataattt
     2341 ggaggaactc acatcagagc aaagacaatt gatggagata catgcagtaa atatgagtga
     2401 accatttaaa gaggcgcatt ttggcaaaga acaaggcttt aaagcggttc ataatattga
     2461 cccacgcaat aacccaatga ttcgcaatgc ctttaaagac ccctcattga caaaatacct
     2521 ggataagacc caggcatttg atttgcactt taatgtttat aaaaaggaaa agcaaagtgc
     2581 actaccagtg ttaaatggta aagaaaaaat tacaatcaaa ccgcatgcta aaattgcttc
     2641 catcatgata ggctctctgg cggcaaatgc ttcagctgat tatgcgaagt atttattgaa
     2701 tcaggggtat gagcatattt ttcttttcgg cgggcttaat gattccattg cagcacgtat
     2761 cgatcaaatc atcaattctt atcctgcctc caggcgtgat gagatacgta aaaaaattat
     2821 tcttctgggg aatcaatctg acgttgaaat ggcaccgata atgacgcgta gtaactgtgt
     2881 ggttatccgt gggggtggtt taagtgtcat ggaacaaatg gccatgccca ttatggatga
     2941 taaaattgta ttattacatc atgaagataa tgaagagggc cctttaacat ctggtctcag
     3001 ttgggaagat gggaatgctg ataaattaat tgagtacctg tccgaaaaag gggcatatgc
     3061 taaaaaaact tcacctggct tgtgttccgg tcatctacat gaggcagaaa aggattttga
     3121 aaagaaatat attacccagc tgaagtcgac aggaaccaag ggaaaagtag atttaaccat
     3181 tcctcagcaa aagaagcgtc ctctgaaaaa agaatgggat aggaaaacgg gatatactga
     3241 atccggacat atattatccc atcagcatag attttttaac acgatacctg aaggaagaga
     3301 gccatttggc tccaaggaag agatacatca ccatgagcag ctttctagtc aaaatttggt
     3361 tagtgtaacc gctggttgat aaaatatgcc tctaatcatc gaggctcgtt ttagagcggc
     3421 cttctctgaa acttgtctgc tcgggatagt atcaatttgt gattgcaata cctactgatt
     3481 gatgcaattc caaaagcaga attgtatttt attggcccac attgcatttt atattttatt
     3541 ttagtacaat tacatgcctt tttgatgatt aaatgaattt ttttgtcatt aattatattt
     3601 tttaataata gagaaatcaa tatgcctaaa attttagata gcaatgcata tcgcgagatg
     3661 gaaaatgagc tcaataatct tttcgagcaa atttataagc atggtaaaaa tgacagaacc
     3721 gcgattacat tacaagctca gcaattttta gataatttga gtgaatccat aagggatcat
     3781 tcccgcttat tgcttaccaa aattgccaga gaagaactca tttattttaa ggaaaaaaaa
     3841 cctggggatt cgcgagagaa atttcaaaat atcgataaga tgagtgatta ctttaacaat
     3901 ttatctgcat tgattaattt ctcggtgatg cagcaagaag atcctactct gcgggttttt
     3961 tattatgata tgtatattca actaatgaat ttttgctatc ttaaaggaga tttgtttgga
     4021 gcaggtgcaa tatatacggg attaatatcc aataacctcc ctaactctat tgattggaag
     4081 caattgagtt ccgagagtcg cttgattttt aaagattgtg aagaaaaatt taagcgatta
     4141 tttaatctct caacgacata caatgaacac actaatttaa aaagacaatt caaaactgct
     4201 ttgatacccg cattaccagc acttctcacg gttcagatat atactaaaga cgcttacgat
     4261 aactctgtca gcgattttga aagaattcag gagaagttac gtcagttgga tatagaacat
     4321 aatcggtttc ttaatcaatt acaaaatgac cattcaacct ggtctaaagc ttattatgat
     4381 tatatgagta atgtttatct cccgcaaagc atattcgaat acacgactct cttaagagag
     4441 tatgaagaac ttgtcacaac gcatggtggc cctattgcga agcagtttca taaggataag
     4501 aattctgaac ctctggtaaa ttttgttgaa tcgacgctat cagaaaatca atcagttctc
     4561 aaagtatctc ctttgcaaaa tgagagaatt gttgaaatgc tttccgagat tgacgtggta
     4621 aaaagtgagt gctcaagccc agctgtgttt gatgacctct cctggaagtt gagagcaatg
     4681 aattgtacct tggtaaaagc taaatctatt ggtgattata cttccaaaat ttttgcaaat
     4741 cttgatactc catacatgga agaaaatacg gatgaagatc cgaaaccagt gcttgtagaa
     4801 gaactgcaaa cggatgatgt gtaccaactt caccatagtg gtatgaaatt gatgcgtgag
     4861 ctcgaacagg ctatagaaga tcataaaccg aaaattactt atcattgttc aggtgaggca
     4921 aactatggtt tctttagtga agccaggagg agagggagac atttgagtcc agaaccaatg
     4981 gatatcgaag tcatggatgt ggatgttatg gattatgatg agaatgattt acgtgatcaa
     5041 accgttatga ttacggtgta atttgtcttg catctataat tgcaatccat cggatagctc
     5101 ttgtagatgc caatccgatt tttatcttat tttgtcctat ttccttgcag aagtttttct
     5161 atctcttctg gtggtatagg cttgctcatt aaaaagccct gaatgtcatt acatcgcctt
     5221 ttcttgagga atttaagctg gttttgcgtt tcgactcctt cggcgatgat attaaaattc
     5281 atactttgtg cgatagcaat aatcgcatca atgatcgctt catctcctgg cgattttgaa
     5341 atattctgaa tgaatgattg gtcgatttta agactatcga cagcgacctg ttttaataaa
     5401 ttcaggctgg aatttcctgt gccaaaatca tccaaaataa tcaacacacc cagtgatttc
     5461 aactttttga tcgttctttg tatttctttg tcaataatca cattttcagt aatttccagc
     5521 tccagacaat gaggttcgac tgaatattct tttaaaagat cctccaccat tgcagggaaa
     5581 tctcgttgtc ttaactgttt acttgctaca ttacaagcga cacgaatttc aggcagcccc
     5641 attgtgcgcc acgtgccaat ttgtttacag gtttcccgca aaacccactc ccctatcggt
     5701 acgataagcc ctgaactttc ggcagtggga ataaaatcca agggtaaaat aatacctttt
     5761 tgcgggtgat tccaccggat taatgcttcc atggaacgaa tctcatgagt attaactgta
     5821 aattgaggct ggtaaaggag gaaaaactca tgatggataa tcgctttttg caagtccaat
     5881 tccagctgaa attgctggtt tgttttttta ttgagtttct ctgtataaaa tgagaattgg
     5941 ttaccgccgc gaaattttgc ctgatacatc gccatatccg cattactgag taaagtgttt
     6001 actgttttgc catccgttgg ataaacgcta atacctatgc tgacatgaat attgatgtct
     6061 ctttcagcaa tatgaaatgg tttttgaaag gatttgagga ttttttgagc tactccggca
     6121 atttgctctt cagaattaaa cgattgaaaa agcattacaa attcatcccc accaagtctg
     6181 gcgagagtat cttcttttcg caccaaagat cgtaatcgtt tagcaacagc acacagtaat
     6241 tgatcgcctg cttcatggct caagctgtca ttgatcattt taaagcggtc caagtcaaaa
     6301 aataatatgg caaatttcat tttatggcgg gcatagctgc tgatagcatg ttcaatacga
     6361 tcaattaata acaagcgatt gggtaaattg gttaatgcgt catgagtagc ctgatactcc
     6421 agtttttcct ttaatcgcgt ttgttcagtg atgtctctga agctccatac acggcctacc
     6481 gtttgcggac caacgcgatg cggtttggaa cagcattcaa ccactttacc atttctaaaa
     6541 tgataaattt gtatactgga atcatcaatg tgaatagaaa attctttaac atgttgggca
     6601 tgttgttctg aattgagtaa ttgcgttgac atatactgaa tgagttggga ttcgctggtt
     6661 tttttcatga tggattcggg tatattccac aatttgacga attgagagtt gtaatcaacg
     6721 attttccctt gtaaatcgac aaccagaatg ccatccgaag aggactcaat cgttgctctc
     6781 aataaggaga aggattgttg caaagattcg gttctttcct ctaccaattg atttaaaaat
     6841 tcagtgtgtt tttgagtttc tctggcaagt acccattttt gtgtcaaagc acttgccagc
     6901 tgtcttaccg caatcacatc aaagggcttt tttagcacca ataaattatc acttaaaccc
     6961 aaggtattca ctgtgctttc ccatgagtag tcggaatagg cactgcatat aacaatttgt
     7021 atttgcgggt caatggccca aatgcgtttg atggtttcta tcccatctat ccccgggggc
     7081 attcttatgt caacaaaggc gagtgggtat ggccggcctt cttcaagtga gcgtttcact
     7141 ttttctatgc cttcaatgcc ttgagtcgct acatcgattt caaattcagg caaagaaatt
     7201 ccaagttgtt ctgtctcatc atcaaacagc tcggcatcaa atttatttaa taatacagag
     7261 ttcttgctga catttaagat tttcataaaa tcctgatgga ttgccggatt gtcatcaatg
     7321 accagaatgc gaaaagattc agtactcatt tctacctcgt actcaaggtt ttgggattga
     7381 tgggcaaatc taatacaaat tgagctcctt tgccattgcc attactttca gcataaagtg
     7441 taccacccag atctctcgca taaattgcag aacagtgcag tccaaagccg tgaccatttt
     7501 tcttggttgt aaatccaaaa gaaaaaattc gatccaggtt atctttttga attccaattc
     7561 cattatcatt tattttaatc ataatatgat ttttttcagt tctcccaatg gaaataacaa
     7621 tttttctgga atcattcgct tcgtgtaata aaacagaatc tttcgcatta gcgagtaaat
     7681 tagtaagaat ttgaaataac ttggatttat cggtgataaa ctcattgatg tccggttcaa
     7741 tctcaatacg gacatcaatt tgatgtttgt ccaatatgac tgattttacc gcattaagaa
     7801 tcacatcatg gattgaaatt ttctctgtta aattcgataa gccgctcaat gattgctgca
     7861 tggctatgat ttctttaata tgattaatgt tagtttccag actgttggct tccttcatgt
     7921 tttcttcgta ttcttcatgc agggttttcg ataacgcgtc caaataggga agaatcaatt
     7981 taccttttgg attctgtaac aagtattcgc ttaattgatt gatgttttcg tcaagcatgg
     8041 tttttatagc ggacagtttt ttataatgaa tgttggatat attctttttc agaataatgg
     8101 cagaaacatt gacgctgttt aaaatattgc ccacattatg aagtatcgag gtggctactt
     8161 ccgacatccc ggcaagtcga gctgttgaaa ccagtttatt attcagttca tgaacttgct
     8221 cttcaataat tttccttttg gagatatcca tcaaaacccc ggaaatttcg tttttatgag
     8281 gattggctct tgctatcgtt cggtaccatt tgtaatcatt gtctccgttt cttactctaa
     8341 gctcataatc ataatcttct ttttctttga gggccttttc gaccacattt tttaaccctg
     8401 ctttatcatt aggatgcaca agatctaaaa atgaagaata gtttagtgcc acattactag
     8461 gcggcaaacc cagaatttta aatagtcctt tggaccatgt tgcggtatct gtgtcagcgt
     8521 cataatgcca ataccccatt tgcgctatat cctgggcgta ttcgagtttt gaattgatat
     8581 ccctcatttc tttagaagaa atatcaatag agcgctctaa taaatatcgg tcttgatcgt
     8641 tttcgtagta tgttttattg atatgtgcaa taaattgagt ccaactctcc agattttgtg
     8701 gtaatccatc agcagtaaaa ccaagttttt ttaactgtcg ctgcaacaac cgatgcattt
     8761 ctgtcattat tactgaattc ctttttggtt tatggactct ctaacagggt cgtaatggtc
     8821 atggtttgat tatgcaattc gcaggtacct gttgccagag gtgataattc accgtaagag
     8881 tagaagccaa tttgtatcga tttaggaggc agtgattgta atgttgtttc tgtctcttcc
     8941 tccgtaagtt cacctaatac caatcttctg ccgacgcaac tgatatcgat tgccaaaaca
     9001 ggttgtgtat tcaagtttcc atttttaaaa agtctatcat tagcgttttc tcccgcctca
     9061 ttggcactga caatcaggcg gtcaaaattg gcacgcatta attgagcata gtatccctta
     9121 ggcaaatcgc cagcaaaaat gagggattgc tcttcttcat tgactcccag aatagtgcgc
     9181 acaatgtgtt gagagtctgg tgcagatggg ttacggatag caagcgggta aagcaagcct
     9241 gccgctggta attcagaagc cctgtcacca agataggttt tatagagttt tagcgcgggc
     9301 tggttgtcca gttcatagag aatatttgct ttagatcgtg taatgactct ttctggtcca
     9361 aaaatatccc aacctcctct tgaaccatgc cctacatgga tttgatcacc gtaaaaccct
     9421 actgccccaa tgcaatggtc tataattttt ccatcgtaaa taatccaggt atttttaaaa
     9481 tccgcaccat ctcctgccag accgcctgta ataataaccc ctttgccagc atgctggttt
     9541 aatcctttaa ttaattcgga accgttgaca tttaagccat cggacaaaac aaatatgctt
     9601 tggggatttt gcttttgatg caatgatttt gataaatgaa ttcctgcctc ataagactct
     9661 tcagtgcttt taacctctgt ttttgccaat tgaagtttcg ttgactcaaa ttgaactact
     9721 gcaaccgaaa tgctgttatc aaaaatatta ggcccaaaaa tttctcctgc agtggagcat
     9781 cctatgattt ttgatttttt ataggcttga gccaattctt caattggttt gctgtttgcc
     9841 ctaaagatgg ggctggcaaa aactaaaatt aaggtatttt cggagtcgag ttcagggaat
     9901 gttgggatac tccacccctg gttttgaatg tattgaaaag attctatttt catcatacgg
     9961 tgtgattcgg gtttatctta tgattaatta tagtctatgt tcaaaaggat caatgaagag
    10021 gagggttatc tattgcaatg ttcatataga aatagaaaaa gcgcaaattc attcctgttt
    10081 atcatcgcga tttatccatt ttttgaagtt gcttagtaag tcattaatca agcggtcatt
    10141 gattcttttt ttgaggtaat aagcatgaaa tgggattgtg agttcaaggt cttttattgt
    10201 taagattttt aacttttctg atagtaaact atcaggcaga taactccagc ctagaccgta
    10261 ctctactata gattttattg catcaatagt actcatttca aaacccaggt ttaacaccag
    10321 ttgatttttt tcaaataggt tatcaatttt atctcgcagc gcagccccct tctcagttaa
    10381 gatggcgggg aacagtgaaa gttccgagag cgacagtgcc gaaattctat gggctagctc
    10441 atgatttgct gccacaacca aattcagttt ttcctcccag agcaaagaat cgcctaataa
    10501 tttttcggac acttctcgtg ccgggcagat aaccaaatca accgtgccat ggttcagaat
    10561 atactccact tctttttcac taatagtttt cagatgtgct tgaatggcag gattgatttc
    10621 tttcaagtat ttaaaaaaac tcggcaatac gtagtgagaa acatacaccg aagcccctat
    10681 gatgatgctt tgtgtgaatt gttgtgagtt tttcagggta tggaccatat tttgatgtgc
    10741 ggaaaacatt tgcctggcat aagggataag ctgtttggct gcttcagtaa aaattaattt
    10801 ggaaccctcg ctaattatca aacgtacatt taactcgtcc tctatttttc gaatgcgttt
    10861 gctcatggcc gattgagtga catgcaattt tttagccgcc agagttaggg aatggtattc
    10921 aatgacagcg aggaaagatt tgatgtctct gatatccata agattttctt tatcttttta
    10981 taaaaagtat agcacaacgg ctatgggtgt ttttcaaacc ggagatggta atagcatgga
    11041 aaggttaaac cattgggtga aactatataa gtagtgctta ctcaacattc ttggcctaca
    11101 catagcagga agtagttggc aattacattc tgtttttaga tttctctatt ccattttgga
    11161 attagtcctt cgtaagactc atgtgctttt aataccccag taatgctcga actgtatcct
    11221 gtaagaacgt attttaatca ataagttttt agcgaattat tcaggagaat atcatgacca
    11281 agaaacattt aaatccagac tctcaaacag atttgggtga cacagagaaa gtggagttag
    11341 aggacacaga tacagttgat attcccactg aaggcgattt gagtccaagt aagatgagat
    11401 ttatttggga gggagatttt agctcatatc agttaaaagc agctgattta aggccaatta
    11461 cgaggcatta taaacaaaaa aatcatattc aaattgtgat aaatggagag catcccttcg
    11521 gtcaggagtt gatctcaaaa aaattagcag ccatggatga gcaaatcaag ggattaaacg
    11581 atgattcact caaatatgcg ttgtatattc aaaaacattg gctacaaccg ggatatctgg
    11641 gagatcaatt ttgtatttct aatatgttcg gcagtgattt ctccaaggtt tataaagagt
    11701 tgaaacaatt gttggcacct tatttaacaa atgatggcga aattaaaaag ggattatcaa
    11761 cgcaggaatt gttggattta aacgaaatag ttaaggccaa atttgctgct ctttttgatg
    11821 ccaagattac ccgatatatt gcatttcttg atgaatccaa gagtgatgtt tgtctggata
    11881 aacaagaagt aatagatgaa attaaaaaaa ttcagtcagg attaaaaccg ggacaattag
    11941 caggatatgt ttatacaaat aatcgaagaa ttggagaaag ccattttgaa gtagtcatta
    12001 tcggtcctga aaccattatc aaacctgtgc attgggggaa tcaaggtaga gaaagtggtt
    12061 tatacagtga agatctgaca gggatgtttg atacaaaaat cgattccttt gtacagacta
    12121 cttcagccat tcctctgcct caagcaggac atgaagagtg cggaacattg ggtattatgt
    12181 atttaaatga attgcttaaa gataatgcca aacaattgca ggaggattca ttgcggtttt
    12241 ctttttatga caagggtaac tcattagcac attttttctt tccttcgccg caagtgttac
    12301 gtttttctca atccagtgct tataactcgg tcattaaagc catgctggaa gatgcgcctg
    12361 atgtagaagt gatacatggc aataaaaaat atagggtaaa aacgttaaga ggtatattgg
    12421 aggagtctat tgaagccgca aaaaaatgtg gtaatagtga attggcagca cgtaatcaga
    12481 agatccttga tgatttacct tctttccgca aacattggct tcaatcttat gatttatgtg
    12541 agcaaaaacg ttcattaatg gaagataggc ataatctgta tcttgcttat acaacgcaga
    12601 gaatgaggcg taaggcagat gagcatgctg gtttgcaagt tgatttcgaa ttagagaatc
    12661 cgcccctcct acctgaaact catgcagaga cagagacaga gacagagaca gagacagaga
    12721 tagagataga gatagagata gaagctacaa atccgactag cgatgaggtg atgcaatcca
    12781 gtagcaatcg cgaacacagt tttacgcctc aacaatcaac tattattata gaagcaatgc
    12841 aaaaggcgct aaccagtatg ggaacaagtc gttttgtaag tggcagagat gataaagaga
    12901 aattgcttgt tcaattcaaa tcagcgtttg aaaacgcaac aaatgggcaa gaacgccatg
    12961 aggctttacg gaattttgta acaacggctt caacgccaag acaaggtgta ctcaattttt
    13021 ttcctgcggc ttacggtaaa actcgctctg ctacagcatt ttatgaacat gttaaaaatc
    13081 aggatccctc tttagttgag gtgttgaaga ctatggatgg agaaaaggga cgcaaagtgg
    13141 tagcgcaatc aaattcatca gacgaaacga aggcaacgat gccagcagac caggcacgag
    13201 gcatcatgca gcattttaaa cgagcaatac aggtagagag aggcagtgaa ttgaaaacag
    13261 gcaatgactc tgtcgactcc caaaatttat gcgacactgg tttaaagatt tagaagagat
    13321 aatctacttt aaaaaaaact gtatgcctaa aaatgtcagg gttaataaaa tggaaatcat
    13381 cgggaaaaca atagggaatg aaatagcctc tggtggtttt ttttctgtca gtttgatttt
    13441 aattaatgag gcatgaatga tgataaaaat acacaatatg atgctgctgg ttaatttagc
    13501 cagggttgta atagggaggg cataggcaaa tagtaaaata ataagagaca ccagtaaggt
    13561 cgagttgact ggtgtttgcg ttttggaata aacaaaggag aatatttgag gggcattttg
    13621 ttgattagcc ataccataaa taagtcgcga agccataatg atttgcacca gaattccatt
    13681 ggtaatagaa attagtgcaa tgattgaaaa caacaagggg gattgtccct gaagcttaat
    13741 aatttcaata aggggcatgt ccgttttgac taaagcattg gatggtatgg atgagacgat
    13801 aacccaggcg actgcaacat ataaaatggt tgcggatatt aaagcaagaa aaatagcttt
    13861 tggaagagtt ttttcaggat ttattgtttc ttcggcggta ttcaccatat cttcaaaacc
    13921 aataaaagca taaaacgcaa taaaagctcc gataagcacc ccatcaaaag aatgaggaaa
    13981 agagaaggat tggatgtcga tagaagctaa tgaataacga ccgtaataaa taatcatcaa
    14041 taatccaccc acttccatga gtgtcatgaa caggatgaga gtcgctgatt cacgaatacc
    14101 ccaaacagca actcccgcta aaatgacaac taaaatagtg atgataaggg aaggcggtaa
    14161 gggtaaaaac aattgaaaat atttgacaaa gccatgggat aaggtggctg ccgagatcac
    14221 acctgttaaa accactatcc aaccaattaa gccagatagc cacctttgct ggaatgctct
    14281 ccgtacgtaa agagcggagc ctgcactaag cggtaacctg gagcttagtt ccgcataaga
    14341 aatggcagta aaaaaagcga ttatggaggc gattaagaag gatagcgggg taaattgtgc
    14401 agcctccttt gcaacttcac caacaagggc ataaattccc gcgcccaata tggttccaag
    14461 tccataaaag gccagcagtg ttaatgataa agtccgtttt aagtgcatta ctcccccatt
    14521 ttatctggag atctatagtc gtagagcaag tcttcttttt tgtttgctta cttttttatt
    14581 tttcgccggt tactaaatag atagtgaaat taaattgata ttcaccatga tatttggctt
    14641 ggcattcatt atcgtaatat tgaaccatag cttcgttgat tttttgtatc tcttcattgg
    14701 gcaattgtcc ttgataaaaa gcaattccat tgacgaattt cctgaaaaca tccagggaag
    14761 ggagggtaat ggattgtcgg catagcttta cttttagctc ctgacagctt aacgactcca
    14821 gttttttttc cagattgttt aagtgagaat aatctacagg agggataaaa tcatgcaagg
    14881 atgcaaattg ccctgatttt tttaaagcgt aataactcat gataaacgga tcgtctcctg
    14941 ctggaaaaag agtaagaaat tttcccccgg gtttcaatgc gttaacgata tttaaaaatg
    15001 ctttttgaat attggcacaa gcccattgca aacaccaaaa agagacgaca tagtcaaatt
    15061 gtaagtggaa gtccattgtg agtacatcgg ctttctggac tgaaaagtta ggatattcct
    15121 tactcacgtc ttgggctaaa tgtaacatat tctcggaagc gtctatacct aatacagagc
    15181 cttgaggcac tttcataaga atattttttg taaaagcgcc attaccgcag ccaatatcca
    15241 atattttatc agtgggattt actttcaggt acgataaata ttcttcagcc acagtcgctt
    15301 gaatataaga tccaatggca tagtcattgg cgggccactc acgtcttgac atgcttattc
    15361 ctttcttttg agatggttat ttattccata attgttcagg tcatcattgt tcatgataca
    15421 tcatggacag tccctaaaat agatcatgaa ttcttttatg aatagtattt ttattgagat
    15481 gaatgactta aatcaagtat agtgagcgct gtaaaaaaat caggatgttt ttacttcaaa
    15541 ctatgaaaag gagtcaggta tgttcaacca gagtcagcaa aaaattgttt gtgtgcttta
    15601 tgatgatcct aaggggggat ttcctccaaa ctatgcaaga gaatccattc cagaattgaa
    15661 acaatatcct gatggtcaaa gtttacctac tcctgattcg attgatttta ttccaggtga
    15721 aatgctaggt tctgtgagtg gtgaattggg gctgagacaa tttcttgaaa acaatggaca
    15781 tcaatttata gtaacctctg ataaggatgg tcctgattca gtatttgcca gggagctgaa
    15841 agacgctact gtcgtcattt cccaaccttt ttggccagcc tatttaactc gtgatcgtat
    15901 tgagagagcg cctaaactta agttggcaat tacagcaggt attggttccg atcatgtcga
    15961 tttgcaagcg gccatggaac ataatatcac tgtttgtgag gttacttatt gtaacagcat
    16021 cagcgttgct gaacatacag tgatgatgat tttggccttg gttcgggatt ttattcccca
    16081 atacaatatg gtaattgatg gcgggtggaa tattgcggat tgcgtcagcc gctcttatga
    16141 tttggaaggc atgcaagtgg gatgtgttgc tgcaggccga attggattag ctgttttaag
    16201 acgcttaaaa ccgtttgctg taaaactgca ttataccgac aggcatagac taccagtaca
    16261 gttggagcag gaactgaatt tgacttatca cccctctgtt gaatccatgg tgaaagtttg
    16321 tgatgtggta tcgattcatt gcccattgca tccggaaaca gaataccttt ttgatgagcg
    16381 attaataaaa cagatgagac gaggctctta tttaatcaat accgccaggg ggaaaatatg
    16441 cgatcagcac gctgtggcca aagctctgga atccggacat ttggctggtt atgccggaga
    16501 cgtttggttt ccacaacccc ctgccaaaaa ccatccatgg cgatcgatgc cacatcacgc
    16561 catgacacca catacctcag gaactacctt gtccgcgcaa gcgcggtatg ccgcaggagt
    16621 cagagaaata ttagagtgtt ggctagggaa caaaccaatt agggatgaat atttgatcgt
    16681 gtctcaaggc cgtttagccg gggttggttc tcactcctac agtgtgggca atagtacaac
    16741 aggtgctgag caagaagtag aattggtttg attcaccgtc gcttaagccg tcttatgtct
    16801 ttctgctttc attcaaagta gccgctgcct ttaaagacag cggcagttca gggaaagggg
    16861 caaatagtgg atgatttaaa atttaaataa attttttgga aggtctggtt ttaaaccaac
    16921 atcgtcttgg ccaaatgaaa agaaacctgg attgtagata gggtgtgttt gctcaaaatg
    16981 ctctagaaaa tcgtctgcgg ctccttcgtt atcccaagtg ttatggtttg agtcgatatt
    17041 tactgtttct gtagtttgat tggctgtttc ttgtctaggt tgaggtactt cttgttcctg
    17101 attctcatat tttcttcttt ttgtattggt atgaatttct ctctccgaaa cgattgatag
    17161 atcaaaataa tccctgtgat ctagtagcga atgaatacag tttgccatgt ctttttcaga
    17221 tgatgggtca cgctctgtag tgcctccttc ccgcactgca tgggtaaaac attgaccttc
    17281 ctgattgatt gaaaaataat tgacaagtcg gccgaatccc tcaaaaacgt aagctatttc
    17341 cagttgttga ttaccctttg ataaggggaa ggccaaattc aaccagggat ccattaaaaa
    17401 ctgttcattg actattagta taaaatgatt attaaaggtc gctaatcgca gggagtgaat
    17461 taagctttct ggtttttgtg cctggtgtaa cagaagtgca aaaacaaaaa aagctttttc
    17521 ctggcaatta ccaaaaccgg agcctctgct atacagagca atcttcagaa ttttatttag
    17581 ccaggtcaaa tcagttcttt ccagagtttc atcagcgaaa atttgagtta tcttaaccag
    17641 tgaagcatcg tctacattat cggtaggtaa atcacaggaa ctgatgtttt gtgccaattg
    17701 attgccaagc agaatcagct cccagagttt tttttcttca gcattgagat ggaatttatc
    17761 attaactgtc cggacttttg ctttaacttc ctgaacttga gcgtgagtaa gggcggcatg
    17821 ctgattcggt ttttttgttt tttcagaaga tttgctgtta agcgctcttt ttggggagcg
    17881 tttttcttgt tgctgattca gtgctttttc tttcattttt tgaaaatcac ctaaaaggct
    17941 atccataaat aattcctgtc tatttttatc agctgctttg gcacaggatt ttgtgaacat
    18001 gttacactct cttttaatag ttaattacga tatgacaagt tgaacctctg atgataatga
    18061 gctggactgc tgtaataaca
//
LOCUS       DBNCWK010000058        18033 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.058, whole genome shotgun sequence.
ACCESSION   DBNCWK010000058 DBNCWK010000000
VERSION     DBNCWK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18033
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.058"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(91..243)
                     /locus_tag="AC06IO_001677"
                     /pseudo
     CDS             complement(91..243)
                     /locus_tag="AC06IO_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            366..3254
                     /locus_tag="AC06IO_001678"
     CDS             366..3254
                     /locus_tag="AC06IO_001678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874857.1"
                     /translation="MAFVLQFCPFLSLLVFIMQLKYDALMPAINLSPSASGKAYTLLN
                     EIREGNHFLKINDFLNMLDLNLLNEVVTSDGERVVDILARFPKGQTLLSYKHELLKKT
                     NLNYTPPKGPYAGTTTLCSLASTKDGRKLLIEKGFLMLGGIDFNACPAEGNYAGRSLL
                     WWLVATSEGREWLLENRVVLKQKLLNFNVAANSGLDRGKSVLWLLAFTSEGRKLLRDK
                     TLNLLAREDLNFHATAEDGKYKGQSVFSLLINNTKTKEFLERKPDLLERISTKEPSTN
                     LNEALDIQLWQLASTTEGIGILKEKYASLLEKGDLNLNVSPKVGKHAGKTLLWLLANN
                     QAGLKLLKDYPQLLEKSDINLNAAPMNGDKAETTVLWLLASTSEGRAILREHPFLLKK
                     TGLNFNATPKWGEDAGVTVLWFLTWSPYSLQSLLEHTNILSTQADLNFNAMKENKTVI
                     RILLVNCIFNGIVTADLPNQIATLLKKHYLSLDLNTLPTDKAGVLFYLTNSEAGLSIL
                     EQVPELLLKPGLNLNTAPTGEQSPLENLISSPKGLQILAKVPDVLLKSDVDLNSATAG
                     RASLLWQLVDKPEGLDILIQNPLIWQMEGLDFNAAPTVGKVDTSVLWILASVPRGQEL
                     LEQMVHLGFAQNGGLNLNLAPQTFPGNTVAYSLVKAGKYDLLTELCRNNFVDLTTLYQ
                     GKSLLEALLERRQYNLLNIILSQYLEPANSIQLEMLLQNSRPEIKQQIKWITDFLKAE
                     RSLQALTVVSDFESAVKHKQYILRKICNLENAHTIYSRDYQSFFNFYMREVGQIRFML
                     NSHFYHKNDTDTPLSTEENVDIRKDYGISNHLIEQWQDFLTIDDLNTISALSGLVEAE
                     QLTLVQNTLAERLVKKNWANPTLAERFTSQGFANPLIAEANTEKAKETPQEGSIQDSA
                     SSPYSNKNHSPTLFRQASSFNSSRSEEKGKQKECGTTKEGNVNASSFL"
     gene            3590..3724
                     /locus_tag="AC06IO_001679"
     CDS             3590..3724
                     /locus_tag="AC06IO_001679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874858.1"
                     /translation="MKNITLLGIDLAQDVFQLHGINEHGKKILGKKVKRSELPALIAN
                     "
     gene            3910..4266
                     /locus_tag="AC06IO_001680"
     CDS             3910..4266
                     /locus_tag="AC06IO_001680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIO9874859.1"
                     /translation="MPTVPIKTIEQQEVQMLHRYQENLIRDRTALATRIRGYLREVGL
                     FLVQGLSQVRKQVPLILEDAENELTSGMREIISSCHKKLLNLDKDIEEYTQKIEHFCK
                     QNPVCERLIKLSELAR"
     gene            4278..4484
                     /locus_tag="AC06IO_001681"
     CDS             4278..4484
                     /locus_tag="AC06IO_001681"
                     /inference="COORDINATES: protein motif:HMM:NF014430.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIO9874860.1"
                     /translation="MYATMGSPTNFKKGRHFAAFWGLAPKEHSSGRKQCLMSISKRGD
                     RYIRSLLIHGGQAVVKNSGKKLII"
     gene            complement(5884..7776)
                     /locus_tag="AC06IO_001682"
     CDS             complement(5884..7776)
                     /locus_tag="AC06IO_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIO9874861.1"
                     /translation="MPDDISRKQFSIGDLYFTNELEVSGLIYEIFYQKSYLSDFLTLS
                     PGAVVFDVGANIGVFSLFVLKQCHYDTEIYSFEPVPATFKCLKKNLARFKHNVHVYNA
                     GIGNVPKDCSIDFTLFGESSVTATYKPTDKIISNYQPLLNYETLLKLSSFQNKPLYYQ
                     LKYLPFLRNYLIKKNYKHQTLETKVRCHLTALGRFIENNQITRIDLLKIDVEGAELDV
                     INSIKPEQFSLIKQLSIEVHDIDNRVEKLVSYLQKQGYITYVDRNPIFAELGFNHHMI
                     YAKLPEPAIITLSEEANNPESYIEARQYFYGLLAGGVRIKLLESMFDLDLFRLFTGKP
                     YLLENDIIKRLELKPVRAKKWLHLLCCEDFLKKIMVGSQVGYQLAKSQLMLGEGYWGF
                     KQYYDFYWQRMANEKLSNILRFTDPKFNVSWPPKTAEEANFLETWMTKTATPLIQTLL
                     ACLDLNQYRSILDVGGGDGAIACALAKAYPHLKITVYNLPESAKIAQKNIDAMGLQKR
                     ISVFAGDFINEEQFPTGFDLILFVRVLWDWDNSRKRKLLNMAYHALKRKGHVAICEGF
                     KELCYDLCLTWEYSYIFADGFDAEVFKTSDEYKIMLQQVGFTPIPTKSISPSEPVPGT
                     VVLAEK"
     gene            complement(7791..8834)
                     /locus_tag="AC06IO_001683"
     CDS             complement(7791..8834)
                     /locus_tag="AC06IO_001683"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIO9874862.1"
                     /translation="MAYHQLSYTFLNHQKLPLIIQPQAKAEGNIDVLNQFLKEENALF
                     KEQLLNYGAIILRGFHVNTAEQFLEVIQSSDLGPNYSYDFCPIPRTQIRENIYTSINI
                     HPSFNLALHNEKSYDQDFPSHIFFNCIHAPQTGGDTALADGNKIWFSLSTVLQKKLQS
                     KGILYRRHYYGTGIQYKIIRSIGKNSGCMTWMERFQTDDQNKVEAMLQQMGVQFRWIQ
                     GNGLIIEQLLPACRNHPISGKLVWFNQSNHANHYYNGTSDYIKSKINNTFSRFILLHK
                     YFHPYMAFYGDGEPLSKQEADCINSAIQKNTVSTAWQPGDVMIVDNFSCLHGKTPHTG
                     NRLILVGLTKYFD"
     gene            9484..10965
                     /locus_tag="AC06IO_001684"
     CDS             9484..10965
                     /locus_tag="AC06IO_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIO9874863.1"
                     /translation="MSFFDLLINKANHLVNQIYKKNYNSITSDQLIWQHIQDCEKQFQ
                     KNQEAIYYSKDILQDLINKRLQTILLHAKTKSPWYKKTLAKINIENFTRERLEELPTI
                     NKTILMENWDAIVTDPKLSLNLVEKHLSKKNDSIDTLYLFSRYQVVSTGGSSGKRGVF
                     IYDWDEWITFHTAFVRYPLYNYERTQIVTTKLNTNPRIVSLFVTNTAIAAYSLAKTFG
                     RVNQNMYFLPMAVTPLNIVITRLNQIQPDILSAGPSYIHKVCQLVQKGQIKIEPKILF
                     VGGEPLFEQTLALIKETWPKVDIFNVFGCTEGMVGLNCRANVDEMHLNDDQCIIEPLD
                     EQNRPVDKGIMVSKMYITNLYNYTLPLIRYENSDEILFLNKTCDCGIHHQLIQTPKGR
                     PGCDFNYPGNIFVHHSLFLGALLPEKNIQEYQVEQTIDGVNIKVVTSGYINKSELQKN
                     IRLRLSKVGLADAQVNITEVPEIKYLYSGKLNRFIPLNSTDKS"
     gene            complement(11263..12579)
                     /locus_tag="AC06IO_001685"
     CDS             complement(11263..12579)
                     /locus_tag="AC06IO_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIO9874864.1"
                     /translation="MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSS
                     EQLQRSGISEQLIANPYYKPYRGILDDLESFDTAPFENTTKNFELLGVQGKVMSHLTY
                     RALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQAIDTQKAIERYYSKVIA
                     PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYL
                     YEENGLFSSDGHCHSFDVSACGTLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNN
                     DGNSSKEGFMAPGIHGQYTCLQSAWKNAGVKPGDLDYIAAHGASTKLGDATEIYALKK
                     AFKDVKKTHCCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAVVPSINFETPNPDFM
                     LEDSPIYIATQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDEC"
     gene            complement(12750..14273)
                     /locus_tag="AC06IO_001686"
     CDS             complement(12750..14273)
                     /locus_tag="AC06IO_001686"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043038 - amino acid activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIO9874865.1"
                     /translation="MRSPDQYTTICSLFEEKVKQCPENTAVYLEDRQISYDAFNKQAN
                     QYARYLQRKGLPLNGIVGIQLARSFEMLVAIFAVLKAGGAYLPIDPMAPVLRNQAILE
                     DSQIKFLIVSDHSLLAATPGLQVLSIHANVSDEDCHNLNLPGKKQDLAYVMYTSGTTG
                     KPKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWEACWWSIAGAGVVL
                     LPPRKEHDIKLFIQMIEQYQINVIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGE
                     ALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKRFDTVPIGKPIQNTQ
                     FFVLDEQLNSSGFEPGELYISGVGLAQGYLNNPVLTQASFINNPYLPGEKMYRTGDIV
                     RWNQEGELLFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMV
                     VFLIANNKQTKIGSAELKSFLKNRLPDYMIPEHYIWLSAFPIKENGKIDKEKLLSFI"
     gene            complement(14290..15417)
                     /locus_tag="AC06IO_001687"
     CDS             complement(14290..15417)
                     /locus_tag="AC06IO_001687"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIO9874866.1"
                     /translation="MPNYQPQYVKQQRLFNQFIEDCLHPHAKIFDKEQNIPRDFFYTL
                     AQNGFLGAGIPKQFGGRQWDELTIGIMHESFAKEFCSLANILTVLGMVSKSLLQFGSK
                     AQKQIWLPRIASGETLAALALTESNIGSDLEHVETQLLDAGDEFILKGSKKYITLGQI
                     ADLFLVLANCQGQLTAVLVEKGTSGLIITPMSNFLGLRSNMLAEIFFDNCRIPKTNLL
                     GSIGIGFNCVIQTALDEGRYTTAYGCVGLGQACLDAALGYTQKRKQFDRRLDEHQLIQ
                     KMITEMVVQVKAARELCFYAGELRQQKNPAYIAETLIAKYAASKMVVFVSNHALQIFG
                     AAGFEETYPVERYYRDAKVMEIIEGTSQMHEILISKLCNDK"
     gene            complement(15487..16341)
                     /locus_tag="AC06IO_001688"
     CDS             complement(15487..16341)
                     /locus_tag="AC06IO_001688"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIO9874867.1"
                     /translation="MKQTKLTLLGAGTMGSGIAQLFAQCGFYVTLIDNLQSQLDKAKA
                     TIAKNLHYLALTQNLESTHSIETILASITFTTKLDELKQSEYIIENITENWDRKKALY
                     QVLKKECSATCILGVNTSSIPITKIASLVDHPQRVIGVHFMNPAPMMPMVEVIKGYHT
                     DELTIEKTRTLLEQVHKKMIVVKDSVGFVSNRAMMIFINEAIFMVQENIASIEDIDVL
                     FKQCFGHKMGPLHTADLIGLDTVLYSLETIYSELNDPKYRPCWLLRNMVDAGLLGQKT
                     KKGFYQYE"
     gene            complement(16450..16689)
                     /locus_tag="AC06IO_001689"
     CDS             complement(16450..16689)
                     /locus_tag="AC06IO_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIO9874868.1"
                     /translation="MEVDHIKNEVKLFLLNSFRIKDIGYSDNIFDTGAMHSLFFIQLL
                     VFIEKKYKIDLEVGEFDLNQLTTIDAIAHFISSKL"
     gene            16889..17878
                     /locus_tag="AC06IO_001690"
     CDS             16889..17878
                     /locus_tag="AC06IO_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIO9874869.1"
                     /translation="MKQTAIVLQGGGALGAYELGVLKYLYASDSFSPNIISGVSIGAI
                     NAATLIGAKDDPIQTLEAMWEELTVFSTQIFPENAESYLSLFGNAAFYHLRTDYWSMP
                     YWTNFYTTMPLQQLLLKYIDFDKLNSSPIHLILTATNVTTGKSAIFENQGTNRTEITP
                     LHVLASGSLPPGFPMTLIGDTYYWDGGLFSNTPLSPVMEKLNPDPNVEKQIIVINLFP
                     SQGKIPTNMPEVFDRVFEIIFSNKIRFNTELTEKINEYIETIQEIEKVIPENSLIRQL
                     PGYKRLLNYKYIQDIIYIESSDEENATASFDFSRKAIQRRINAGYKDAQRIFN"
BASE COUNT         5849 a         3417 c         3208 g         5559 t
ORIGIN      
        1 ttaaaatcct aatatagtag gattatatta aagataatag gcacttcttt aatcaacttc
       61 ctccaagatc tcttcaactc ctatcatttg tcacatcaaa taatccgaaa tcctttgcat
      121 aggttttcta agtcgctcca catcgcgaat tccatatcct gcagttacat cgttgatcag
      181 cttatgatta agtaggcgtt tcaatgcata agccggcaaa tcaaggcttt ctgcaattga
      241 tttcatcttt cgatgaaacc attccttaat cccattatta ggaagcaaag tgggcaccac
      301 ccctacttaa gctggtatac attgttctct tcgcttgcca cttaatggat catatagatt
      361 attttatggc ttttgtgtta caattttgcc cttttttgtc tttattggta tttattatgc
      421 agcttaaata tgacgcgctt atgcccgcta ttaacttatc tccttcagct agtgggaaag
      481 cctatacatt attaaatgag ataagagaag gcaatcactt tttaaaaatc aatgacttcc
      541 ttaatatgct agacttaaat ttgctcaatg aagttgttac ttctgatggt gaaagagtag
      601 tagatatttt agctagattt ccgaaaggcc aaacattgct tagctataag catgagctat
      661 taaaaaaaac taatctaaat tatacccctc ctaaaggccc ttatgctggt acaaccactt
      721 tatgtagctt ggcttctact aaagatggtc gaaagctttt aatagaaaaa gggtttttga
      781 tgctaggagg cattgatttt aatgcatgtc cagcagaagg caactatgcg ggtagatcac
      841 ttttatggtg gttagtagct acaagcgaag gcagagagtg gctacttgag aatcgcgttg
      901 tacttaaaca aaaattatta aattttaatg tggcagctaa cagcggactt gataggggaa
      961 aatcagtgtt atggctccta gctttcacaa gcgaaggccg aaagctttta agagataaaa
     1021 cacttaattt actagcgcga gaagatttaa attttcatgc aacagcagaa gatgggaaat
     1081 acaaaggcca atcggtattc tcacttctta ttaataatac taagaccaaa gagtttctgg
     1141 aaagaaagcc tgatttatta gaacgaataa gcacaaaaga accatctacc aatctaaatg
     1201 aagcgttaga tatacagctg tggcagcttg cttccacaac agaagggata gggattttaa
     1261 aggaaaaata tgcttcatta ttggaaaaag gagacttgaa tcttaatgtc tctccaaaag
     1321 taggtaaaca tgctggaaaa acgctgttat ggctattagc taacaatcaa gcaggactaa
     1381 aacttttaaa ggactatcct caattattag aaaagtcaga tataaatttg aatgctgcac
     1441 caatgaacgg cgataaagct gaaactactg ttttatggct attagcgagt acctcagaag
     1501 gacgggcaat tctaagagag cacccatttt tactaaaaaa gactggtcta aattttaatg
     1561 ctacgccaaa atggggtgaa gacgctggcg taactgtttt atggttttta acctggtcac
     1621 cttattctct gcaatctttg ctagaacata ctaatatttt atcgacacaa gcagatttaa
     1681 attttaatgc catgaaagag aataaaacgg tcattagaat tctacttgtt aactgcatat
     1741 tcaatggcat cgtaaccgca gatttaccaa atcaaattgc tacattatta aaaaaacatt
     1801 acttatcttt agatttaaac actttaccaa cagataaggc aggggtttta ttttatttaa
     1861 ctaacagtga agcgggttta agcatattag agcaagtacc tgaattatta ttaaagccag
     1921 gcttaaattt aaatacagca ccaacaggtg agcaaagccc actagaaaat ttaattagca
     1981 gcccaaaagg cttacaaatt ttagcaaagg ttccggatgt attattaaaa tcagatgtag
     2041 atttaaattc agcaacagcc ggtcgtgcaa gcctgctatg gcaattagta gataaacctg
     2101 aaggcctaga tattttaata caaaatccat tgatatggca gatggaaggt ttagatttca
     2161 atgctgcacc aactgttgga aaagttgata ccagtgtttt atggatttta gcttctgtac
     2221 ctaggggaca agaattactc gaacagatgg ttcatttagg ctttgcacaa aatggcggtt
     2281 taaatcttaa tcttgcacca caaacgtttc ctggcaacac agttgcgtac agcttagtta
     2341 aagctggtaa atatgattta ttgactgagc tttgtagaaa taattttgtt gatttaacta
     2401 ccctttatca aggtaaaagc ttattagaag cattattaga acgtaggcaa tacaatctat
     2461 taaatattat tttaagccaa taccttgagc ctgccaatag tattcagtta gaaatgcttc
     2521 ttcagaatag taggcctgaa ataaaacagc aaattaaatg gataaccgat tttctaaaag
     2581 ctgagcgtag tttacaagct cttactgttg taagtgattt tgaaagtgct gtaaaacata
     2641 aacagtatat tttgagaaaa atctgtaatc ttgaaaatgc acatactatc tatagtagag
     2701 actatcaatc tttcttcaac ttctatatga gagaggtagg gcagataaga tttatgttaa
     2761 actctcattt ttatcacaaa aatgatacgg acacaccttt atctacagaa gaaaacgtag
     2821 atattagaaa agactatggt atttctaatc atcttataga acagtggcag gattttctta
     2881 caatagatga cttaaatacg ataagcgcct tatctggact tgttgaagca gagcaattaa
     2941 ccttagttca gaatacctta gctgaacggt tagtgaaaaa aaactgggca aaccctacgt
     3001 tagctgagcg ttttacaagc caaggatttg ctaatccgtt aatagcggaa gcaaacactg
     3061 aaaaagcaaa agaaacacct caagaaggct caatacaaga ttcagcaagc tctccatatt
     3121 caaataaaaa ccactcacca accttgtttc ggcaggcctc ttcttttaat tcaagtcgaa
     3181 gcgaagaaaa aggaaaacaa aaggaatgcg ggacaacaaa agaaggtaat gtcaatgcat
     3241 catcatttct ctgagaaact ggagcccatt ttatttaatt aatcatttca tggcagagtt
     3301 tatttcccgt cgtgaaatga tttgaatatg cccaaagaat gaaggctaat tgccttatct
     3361 gctaagatga tcatctttta tcattcctag ttcgccttgg agtcttattg attaaaaatg
     3421 accttatagt gtttgtatgg cctaaaatat cttgtgacag tgacagctta tccataaaaa
     3481 cactgaggtg gtcaagcaag caatgatatg gactcatcct cccttttacc cggctattag
     3541 atgagccaca atagtaaggt ctaccaaacc ttatcggaag gagagtccaa tgaagaatat
     3601 tacgttatta ggtatcgatt tggcacaaga tgtatttcaa ctgcatggaa taaatgaaca
     3661 tggaaaaaag atcctgggga aaaaagtaaa gcgctcagaa ttgcctgcgt taattgcaaa
     3721 ttaaccttct tgcaaaatag taatggaagc ttgtggtagt tcaaattact ggtctcgaaa
     3781 attcatgtcc tatggtcatg aggtattcca aattagtccc caacatgtga agccattcgt
     3841 ccagggaaat aaaaatgata agataagaat gatgcacgag caattgtcat agcggcacaa
     3901 caagataata tgcctacagt tccaattaaa acgatagaac aacaagaagt acaaatgtta
     3961 catcgttatc aagagaactt aatacgagat agaacggcat tagcaactcg catacgaggc
     4021 tatttgagag aggtaggctt atttttagta caaggattat cacaagttcg aaaacaggtt
     4081 cctctgatac tagaagatgc ggaaaatgaa ttgacgagtg gcatgcgaga aataatatct
     4141 agttgtcata agaagttact gaatttagat aaagacatag aagaatacac acaaaagata
     4201 gagcattttt gtaagcaaaa ccctgtgtgc gaacgcttaa taaagctgtc agagttggct
     4261 cgataatagc gagtattgtg tatgcaacga tgggtagccc aactaatttt aaaaagggac
     4321 gtcactttgc tgccttttgg ggattagctc ctaaagagca ttccagtgga agaaaacagt
     4381 gcttaatgtc tatcagtaaa cgtggagatc gctacatacg cagtttactc atacatggtg
     4441 gccaagcagt tgtaaaaaac tcgggaaaaa aactgattat ttaaattgtt ggatacaaag
     4501 ccttcataaa gagcgagggt acaataaagc tgctgttgaa gtagttaata aaaatgctcg
     4561 tcatatgtgg gcgattatgc cttatgacga taagtatgtc gatcatttga tgctttgcgc
     4621 ttaaacaaga gtttataaaa tgataaatca gcatcgcttc ttgctgcgcc tatctttttt
     4681 gtaatcagta aaaacattgc ttacaaaaaa gccagggcta taaacatagc gaacacatgt
     4741 ggagataaag tactaacctg agttttgtaa ataacagctg atgtggaaat ggtaaaatcc
     4801 actctgtcaa tacctgtagg ctacggtggg cattattgcc cgtgcaagcg ataaaggaag
     4861 cagagtgcga atatcatcaa ggctcgagtg aataaataag tctctctttg gatggtcgga
     4921 tatacgtatg caaatacttc acttttcacc agctaattac ttgcaaaaac gtatgagtcc
     4981 atatacgtag cctgtttaca agtcaggcta cgctagatgg cacaaattta ttaaatttat
     5041 tctatcataa aggatgagat catttacaga tattgacctc ttgtacttga aatcttgctt
     5101 aaatggagcg aggattataa aaatatcctc acttaagcta aaaaaatgaa tttatatgct
     5161 gccaaaattg tcccggtaga agagcacatt gccaaaacca cctccccaca atttatgtct
     5221 gtatttttat tagttcaaat tttaagcata tcaattggtg tggcctttct ttttgttgca
     5281 ttttatatgc ttcgtccaag aaaaaaagac caaatgctca tccaaaatca atttttactt
     5341 tatttaatct tagggctttt tttcattttt tcccctttcc tactcgaata tctaaggtaa
     5401 ttagcaagta tagatcgagc caagacctat aaccgtcagg ctaaggatta aaagcttgtc
     5461 atgttcaagc gcagccacac caggaggtat tgagtctgcg atatttaata attcgctata
     5521 acgttttgag cccagaaaat tattgctcaa atcaagagta gttacatctt cagggaagag
     5581 ataaaagctt gtgccaaatt gttgtcagtt aatgttatct tataaatcat agttaaacct
     5641 caccaataat gttaatcaat taaaaattgt gattgttatt aacataaatt gaatatatat
     5701 tatgcatata aatttcttat atgctttgcg ttaaagtgag agttgcaacg cttactaaga
     5761 aaattctgct aacaggatgt gtccctttta tcccaagtga acattttgta tggggatacc
     5821 taaccctgtg tttatggagg ttgtgttcaa tgggtctaag tcaactcata taattgatga
     5881 aaattatttt tctgctaata caaccgtccc aggcactggc tcagaaggag aaatggattt
     5941 ggttggaata ggagtaaaac ctacttgctg cagcatgatt ttgtactcat ctgacgtttt
     6001 aaaaacttcc gcatcaaatc catctgcaaa aatatagctg tattcccatg tcagacaaag
     6061 atcatagcaa agttctttaa agccttcaca gatagcaaca tggcctttcc tttttaaagc
     6121 atgatacgcc atatttaata atttcctttt tctggaatta tcccaatccc acaagacacg
     6181 cacgaataaa atcaaatcaa atcctgtagg aaactgttct tcgttaataa aatcacccgc
     6241 aaatacacta atgcgcttct gtaatcccat tgcatctatg tttttttggg ctatttttgc
     6301 tgattcgggt aaattgtaaa cagtaatttt taaatgcgga taggccttag ccagtgcaca
     6361 agcaatagcg ccatcaccgc ccccgacatc caatatggat cgatattgat ttaaatcaag
     6421 acaagccaat aaagtttgta taagtggtgt tgctgttttt gtcatccatg tttcaagaaa
     6481 attagcttcc tcggcagttt tcggtggcca ggaaacattg aactttggat cagtaaaacg
     6541 caatatatta cttaattttt cgttagccat cctctgccaa taaaaatcat aatattgttt
     6601 aaatccccaa tacccctctc ctagcattaa ttgacttttt gccaattgat acccgacttg
     6661 cgaaccgacc atgatttttt ttaaaaaatc ttcacaacaa agcaaatgta accatttctt
     6721 tgctcgaaca ggtttcagtt caagtctctt gataatgtca ttttccaata aataaggttt
     6781 gcccgtgaac aaacgaaaca aatcaagatc aaacatagat tccagcagtt taatcctcac
     6841 cccaccagca aggagtccat aaaaatactg gcgtgcttca atataactct caggattatt
     6901 tgcttcttca cttagtgtga taattgccgg ttcaggcaat ttggcataga tcatatgatg
     6961 attaaaaccc agctctgcaa aaattgggtt tctatcaaca taagtaatat aaccttgctt
     7021 ttgtagatag gaaacgagct tttcaacccg attatcaata tcatgaactt caatgcttag
     7081 ttgtttaata agcgaaaact gctctggctt tatgctgttt attacatcaa gctcggcacc
     7141 ttccacatca atttttagta aatcgatacg agtgatctga ttattttcaa taaaacgccc
     7201 taatgcagtc aggtggcatc tcactttggt ttctaaagtc tgatgtttat agtttttttt
     7261 aatgagataa ttacgcaaaa aaggcaaata ttttaattga taataaagcg gcttattttg
     7321 gaaggaagag agctttaaca atgtttcata atttaacaat ggttgataat tggagatgat
     7381 tttatcagtt ggtttgtaag tggcggtaac ggagctttcc ccaaagaggg taaaatcgat
     7441 actacaatct tttggaacat ttcctatccc agcattgtaa acatgcacat tatgcttaaa
     7501 ccgcgccaaa ttctttttta aacacttaaa agtagcaggg acaggctcaa aactataaat
     7561 ttcagtatca taatgacatt gctttaaaac aaataaggaa aaaacaccaa tattcgcccc
     7621 gacatcaaaa acaacagccc ctggacttaa agtaagaaaa tccgacaaat agcttttctg
     7681 atagaaaatc tcataaatta aaccactgac ttccaattca ttagtaaaat acaaatctcc
     7741 aatactaaat tgttttcgtg aaatatcatc aggcattgat atgggtccct ttaatcaaaa
     7801 tattttgtca gtccaaccag gattaatcga tttcctgtat ggggtgtttt tccatgcaag
     7861 caggaaaaat tatcaactat cataacatcc cctggttgcc aggctgtgga taccgtattt
     7921 ttttgtattg cagagttaat gcaatctgct tcttgcttcg ataaaggttc accgtcgcca
     7981 taaaaagcca tataaggatg aaaatattta tgtaacaaga taaaacgaga aaaagtgtta
     8041 ttaatcttgc ttttgatgta atcactggtt ccattataat aatgattagc atggttactt
     8101 tgattaaacc agactagctt tccgctgatc ggatgattcc ggcaggctgg aagcaattgt
     8161 tctatgatta gcccattgcc ttgaatccat cgaaattgca cacccatttg ctgaagcatc
     8221 gcttccactt tattttggtc atccgtttgg aatcgctcca tccatgtcat gcacccagaa
     8281 tttttgccta ttgatcgaat tattttgtac tgtattccgg taccgtaata atgccttcga
     8341 tataatatcc ctttagactg caattttttt tgcaaaaccg ttgataaaga aaaccatatt
     8401 ttgtttccat cagccaatgc agtatctccg ccggtttgcg gggcatgaat gcaattgaaa
     8461 aaaatgtgac tgggaaaatc ctggtcatat gatttttcat tatgtaaagc aagattaaaa
     8521 ctcggatgga tatttatcga agtataaata ttttctctga tttgtgttcg cggaatcggg
     8581 caaaaatcat aagaataatt tggtcccaaa tcgcttgact ggatcacttc taaaaattgt
     8641 tcagccgtat taacatgaaa accacgtaag ataatggcgc cataattgag aagttgctcc
     8701 ttaaacaagg cattttcttc tttaaggaat tgatttaaaa catcaatatt accttccgct
     8761 ttagcttgag gctgaataat taaaggtaat ttttggtggt tcaaaaaagt atagctgagt
     8821 tgatgatatg ccataaaatt cctcactgac aatccggagg tgtactatca gactttcaga
     8881 gtgtaggtcc ttcctgattc tataagtggc tccctctttt ttacctatat tacaaaaatt
     8941 accgcatcat ctttgacgaa tacctatatt aaatagtata gacactgacc ttgctatgca
     9001 atgaaaatcc accagatttt gccaaagaag ctcctgccga atcattcaaa caagacttac
     9061 gaaaaactct aaggtcaaag caaaaaatgt ttttaatgag ggagtttaga taaactaaat
     9121 gaccgaatta gaaacatttt ttaacataga gattggggtt tttcgtaagt cctgtcaaat
     9181 tatctgtgga tagtatcgac tttaacatca tcatcgatga gaactctttg caattgcata
     9241 aatcattaaa ctgcactgaa aatgactgat aacggcacta ccttgtgtaa tagaatatcc
     9301 tgcttgattt taagctataa tcttcttagg tattttaatc ttatggtgac tgccaatcaa
     9361 aaattaatcc aggctaccta ttgattaaaa tcccgtttag gaatatcacc actaattatg
     9421 ggttctctca aagaccatgc cttatagtgt aaggatagcg ccctggggaa aaaggggagt
     9481 aatatgtctt tttttgacct cttaataaac aaagccaatc acttggttaa ccaaatctat
     9541 aagaagaatt ataactccat aacttcagat caactgatat ggcagcatat tcaagactgc
     9601 gagaaacagt ttcaaaaaaa tcaggaagcc atctattatt ctaaagacat actgcaagac
     9661 ctaatcaata aaagactgca aacaatatta cttcacgcaa aaacaaaatc tccctggtat
     9721 aaaaaaacac tggcaaaaat caacatagaa aactttacca gagagcgatt agaggaactc
     9781 cctactataa ataaaaccat tttaatggaa aattgggatg caattgtcac tgatcccaaa
     9841 ctttcattaa atctggttga aaagcatctg agtaaaaaaa atgacagcat tgatacactg
     9901 tatttattct cgcgctatca ggtcgtgtca actggaggaa gcagtggaaa acgcggcgta
     9961 tttatttatg attgggatga atggattaca tttcacaccg catttgtccg ctatccactt
    10021 tacaattatg aacgcacgca aattgtaact accaagctaa acacaaaccc aagaattgtc
    10081 agcctgtttg taacaaatac agcaattgca gcctattctt tagcaaaaac gtttgggcga
    10141 gttaatcaaa atatgtattt tctgcctatg gctgtaaccc cattgaacat agtgattaca
    10201 cggctcaatc aaatccaacc cgatatctta agtgccggcc cttcatatat tcacaaagta
    10261 tgtcaattgg ttcaaaaagg acaaataaaa attgaaccca aaattctttt tgttggagga
    10321 gaaccgcttt ttgagcaaac cttggcttta atcaaagaaa cctggcctaa agtggacatt
    10381 tttaatgtat ttggttgtac tgaagggatg gttgggctaa attgccgggc gaatgtggat
    10441 gagatgcact tgaatgatga tcaatgcatt attgaacctc tggacgaaca aaatcgcccc
    10501 gttgacaaag ggattatggt ctccaaaatg tatataacta atttatataa ttacacttta
    10561 ccattaatcc gctatgaaaa ttcagatgaa attttatttt taaataaaac ctgtgattgt
    10621 ggcatccacc atcagcttat acaaacccct aaaggtcggc ctggatgtga ttttaattat
    10681 cctggtaata tttttgtaca tcattcgctt ttcttaggag cactcttgcc agaaaaaaat
    10741 attcaggaat atcaggtcga acaaacaatt gatggggtta atatcaaggt tgtgaccagt
    10801 ggttatataa acaagagcga attacaaaag aatatacgct tacgattaag caaagtgggt
    10861 ttagcggatg cccaagtcaa tatcactgag gtacctgaaa ttaaatattt gtattcgggt
    10921 aagctgaatc gttttatacc attgaattcg actgacaaat cttagtgaca ggaattattt
    10981 cctgtgatga aaacacagct agccgccatt tcttgattta ctcttatttg tcgatgagga
    11041 aatctcaaac aatttaaata ccaagatctg tatagcccaa tccgcatctg cacgtctcaa
    11101 gcctgtcaga gatggccttc gagacgtgtc tttagccact cctcaagccg aacgtaacaa
    11161 aatgacatgt tcctcgctgc ttaaattcta catagctctc taacgagcaa tcggcatcag
    11221 gtaaactttt aacaggaata aaaacaaaat atcaagagaa ccctagcatt catcatagct
    11281 acgcaaaaca agatgtgtgt tacttccccc tgcgccataa ctggaaacac cggccaaata
    11341 gtgtttttta atttgaggga aatcctgaaa ttgagtggca atatatatag gcgagtcttc
    11401 caacataaaa tctgggttag gggtttcaaa attaattgaa gggaccacag ccttattttt
    11461 gagcataagc acagttttaa tgattgacgc cataccggac acaatgccag tatgccccag
    11521 attactaatt actgagctta ttggacaaca atgtgtcttt ttaacgtctt tgaacgcttt
    11581 ttttaaggca tatatttctg ttgcatcacc cagtttggtt gaagcaccat gagctgcaat
    11641 ataatccaga tcgccgggct taacccccgc gtttttccaa gctgactgta agcaagtata
    11701 ttggccatgg atccccggtg ccataaatcc ttcttttgaa gaattaccat cgttattcat
    11761 ggcggatccg ataatcacag catggattag atcattatcc gcaatggcat cttcaagccg
    11821 ctttagaaca accaatccag caccgtttga atataaggtg ccacatgctg aaacatcaaa
    11881 agaatggcaa tgtccatcgg aggaaaacaa gccattctct tcataaagat atccaacttc
    11941 ctggggtaaa tcgattaagc acgcacctgc aattgccata tcacattgat gactgtttaa
    12001 ctctctacag ctttggatta cggctgtcag agaagacgag caagctgtat ataaatcgat
    12061 agaactaccc tgaaatccaa attgataaga aatatatggt gcaatgactt ttgaataata
    12121 cctttctatc gctttttgag tatcaatcgc ctggtagtag gcggctccca ggaaatggga
    12181 ggcgggttga ttatttgcgc caatataaac agccgtattt tttatgtagt tatttgaatg
    12241 atgctcctga cttctcatgg tgctcagagc ccgataagtt aaatgcgaca tcactttgcc
    12301 ttgcacaccc aacaattcaa aatttttggt ggtattttca aacggagcgg tatcgaatga
    12361 ctccaggtca tctaatatcc ctctgtatgg tttataatag ggattagcga ttaattgctc
    12421 tgaaatacct gatcgctgta attgttctga ggaaaattta gtgactgctt ctttaccaaa
    12481 cttcaataaa tcccaatatt catcaagatc attagcccca ggaaatttta cagccatacc
    12541 aacaatagca attttgtttg agtagttatt gtcactcata atttatggct ccatttcttt
    12601 ggccgcacca agaatttaat aaatgctaac tagacgacgt acgacgggtt cataacataa
    12661 agcatagttt atattatttc tgcaaccggc ttttaattaa gtataagtca ttttccctga
    12721 ttatttttat ttctcggaca ttgctcatgc taaatgaaac tcaatagttt ctctttgtct
    12781 atttttccat tttcttttat tggaaatgca cttaaccata tataatgctc tggtatcata
    12841 taatccggga gcctgttttt taaaaaggat ttcagttctg cgctgccaat ctttgtttgt
    12901 ttgttattag caatcaggaa tacaaccatt ctatgatcaa gacaatctac tttctcacag
    12961 accacgacgg catcatggat attgggatgc gctaataaat gatgctgaat ttctcctaat
    13021 tcaatacgta tgccacgtaa tttgacctga tcgtccttgc ggcctaaaaa taataactca
    13081 ccctcttgat tccaacggac tatatctcca gtacgataca ttttttctcc aggcaagtaa
    13141 ggattgttta taaacgatgc ctgagtcagc actggattat ttaaataacc ctgagctaat
    13201 ccaactccgg aaatataaag ctctccaggt tcgaagccgc ttgaatttaa ctgctcgtct
    13261 aacacaaaaa attgagtatt ttggataggc tttccaatgg gtacagtgtc aaaacgtttg
    13321 tgcttttcac aaataaaaca ggaaacatct attgtggcct ctgtgggccc gtataaattg
    13381 actaatattg ttttttgatc ttgaaataac tggttgaata gattaacact tttggcatcc
    13441 agcgcctctc cacttgaaaa tacgtatttt aaagagctta agcgatttat cgaaaaatcc
    13501 tgttcgatgt aacttaaaaa tattcgtaat acggagggta caaaatgaat gacattgatt
    13561 tgatattgct caatcatttg aataaacagc ttgatgtcat gttcttttct tggcggtaaa
    13621 agtactactc cggcgcccgc gatagaccac caacaggcct cccagacaga gatgtcaaaa
    13681 caaatatgtg tcttatgaaa tactacatca tgactttgca agggaaatgt gttttgcatc
    13741 cataaaatac gattaagcaa agcttgatgt gaaatcagga ctcctttcgg cttgccagta
    13801 gtgcctgaag tatacatcac atacgctaaa tcctgttttt tcccgggcag gtttaaatta
    13861 tggcaatctt catcgctcac attggcatga atgcttaaca cttgcaaacc cggggttgcc
    13921 gccaataatg aatgatcact gacaattaga aatttgattt ggctgtcttc aaggatggct
    13981 tgatttctca gaacaggagc catgggatct atcggcaaat aggcaccacc tgcctttaat
    14041 actgcaaaaa tagccaccag catttcaaaa gagcgcgcga gttgaatacc gacaattcca
    14101 tttaatggca acccttttct ttgcagatac cgcgcgtatt gattggcttg tttattaaag
    14161 gcatcatacg aaatttggcg atcttccaga taaaccgctg tattttccgg gcattgcttt
    14221 accttctcct caaataagga gcaaattgtc gtgtactgat ctggggatcg cattgtttaa
    14281 taatatcctc tatttatcgt tgcaaagctt tgaaatgagt atctcgtgca tttgtgaggt
    14341 accttcaatg atctccatta ccttggcatc tctgtaatat cgctcaacag ggtaagtttc
    14401 ttcaaagcca gctgcaccaa atatttgcaa agcatggtta gatacaaaaa caaccatttt
    14461 tgaagccgcg tattttgcaa ttaatgtttc agctatgtat gctggatttt tttgctgtct
    14521 gagttctccg gcataaaaac acaattcccg ggcggcttta acctgtacca ccatttcagt
    14581 tatcattttt tgaatgagct gatgttcatc taatctgcga tcgaattgtt ttcttttttg
    14641 agtatagcca agagccgcat ccaggcaagc ttgccctaaa ccaacacatc catacgctgt
    14701 tgtataacgt ccttcatcta aagcagtttg aataacacag ttaaagccga taccgatact
    14761 acccaacaga tttgttttag gaattcggca attatcaaaa aaaatttctg ccaacatatt
    14821 agaacgtaat cctaaaaaat tagacatagg tgtaataatt aagcctgacg tacccttttc
    14881 aactaaaacc gcagtcagtt ggccttggca atttgctaaa actaaaaaca aatcagctat
    14941 ttgccctaag gtaatgtatt ttttacttcc ttttaaaatg aattcatcac ctgcatccag
    15001 caactgagtt tcaacatgct ccaaatcact accgatatta gattcagtta aagccaatgc
    15061 agctaaagtt tctcctgagg caatccgtgg caaccagatt tgtttctgag cttttgaacc
    15121 aaattgcaaa agtgacttac tcaccatgcc caatactgtt aggatatttg ccaaagaaca
    15181 aaattcttta gcgaaggatt catgcatgat accaatggtc aattcatccc attgccgccc
    15241 gccaaattgt ttgggaatac cagcgcccaa aaaaccattt tgggctaatg tataaaaaaa
    15301 gtcgcgcgga atgttttgtt ccttatcaaa aatttttgca tgggggtgaa ggcaatcttc
    15361 tataaattgg ttaaatagcc gctgttgctt cacgtattga ggctggtaat ttggcaaaat
    15421 tgctctccct agtgtttgtt tacaatttaa ccatccgggt tgaaatagcg aatggtaaac
    15481 taccccctat tcatattgat aaaatccctt ttttgttttt tgcccaagaa gcccagcatc
    15541 aaccatgttt cttaataacc agcatggtcg atattttgga tcgtttaatt ctgaatagat
    15601 ggtttccaaa gaatagagaa ccgtatccaa cccaattaaa tcagccgtat gcagcggccc
    15661 catcttatgc ccaaagcatt gtttaaatag aacatcaatg tcttcgattg atgcaatgtt
    15721 ttcctgtacc ataaagatgg cttcgttaat aaaaatcatc atggctcggt tactcacaaa
    15781 accaacagag tccttaacaa cgatcatttt tttatgtact tgttctagta atgttcttgt
    15841 tttttctatt gtcaattcgt cagtgtgata tcccttaatg acctctacca taggcatcat
    15901 cggtgccggg ttcataaaat gaacacctat aacgcgttga ggatgatcaa caagtgacgc
    15961 gattttagta atagggatgg atgacgtgtt tacccccaaa atacaggttg cgctacattc
    16021 tttttttaat acctgataca acgctttttt ccgatcccaa ttctcagtga tattttctat
    16081 aatatattcc gattgcttaa gttcatctaa ctttgttgta aaggtaattg atgcaaggat
    16141 agtttcaata gaatgtgtcg attctaagtt ttgagttaat gccagataat gtaaattttt
    16201 agcgatagtg gcttttgctt tatctaattg agattggaga ttatcgatta aagtgacata
    16261 aaatccgcat tgtgcaaata gttgggcgat acctgaaccc attgttcccg cgccaagaag
    16321 agtaagtttt gtttgtttca tgccgtttgt ccaccaacgc ttatcacagc tattcgtatt
    16381 gaattataag taaaagctca actctggctg tttgccctaa atccaacata ggggactgac
    16441 ctattacaat tatagtttac tggaaataaa gtgagcgata gcatcaatag tagttaactg
    16501 gttcagatca aattcaccga cctcaagatc aattttatat ttcttctcta taaaaactaa
    16561 taattgaatg aaaaataaag agtgcattgc gcctgtatca aaaatattat ccgaataacc
    16621 aatatcctta attcgaaaag aattgagtaa aaataatttt acttcatttt taatatggtc
    16681 aacttccatc aaatcttcct gttttataag aatagtctta ttcagatatt ggtaatggaa
    16741 ttattcataa taatattttt cccaggaaag tcaaagtgct aaaccaatac ttagaaagga
    16801 agggcatcag aggacaatga ctcagcatca ttttcattat agttacctgc tatacttgtt
    16861 gatacagcgt gaaatgataa ggacatccat gaagcaaaca gctattgtac ttcagggagg
    16921 cggagcactg ggagcttatg agttaggcgt attaaaatac ctctatgcat cagacagttt
    16981 ttcgccaaac attatttcag gcgtttctat tggggccatc aacgcagcga cactgattgg
    17041 ggcaaaagac gatcctattc agactctgga agcaatgtgg gaggagttga ctgttttttc
    17101 tacccagata ttcccagaaa atgccgaatc ttatctgtca ctctttggca atgctgcttt
    17161 ttaccatttg cgtaccgatt actggtctat gccttattgg accaattttt ataccacaat
    17221 gcctcttcag caattattat taaaatacat tgattttgac aagctgaata gcagcccaat
    17281 ccatctaatt cttactgcaa ctaatgttac aacaggtaaa agcgccattt ttgagaatca
    17341 gggtactaac cgtacagaaa tcacaccttt gcatgtattg gccagcggca gtttgccccc
    17401 tggctttccc atgacactca ttggagatac ttattactgg gatgggggtt tattttccaa
    17461 tactccgcta tctccggtta tggaaaaact taaccccgat ccaaacgttg aaaagcaaat
    17521 tattgtgatt aatcttttcc ccagccaggg aaaaataccg actaatatgc ctgaagtttt
    17581 tgacagagta tttgaaatca ttttttccaa taaaatccgt tttaataccg aattaactga
    17641 aaaaattaat gagtacattg aaactataca ggaaattgaa aaggtgattc ctgaaaatag
    17701 cttgataaga cagctcccgg gctataaacg actgcttaat tacaaatata ttcaggatat
    17761 aatttatata gaaagctcag atgaagaaaa tgcaaccgct tcttttgatt tttcccgtaa
    17821 agcgatacaa aggcgcatta acgcaggata caaagatgct cagagaattt tcaattgaaa
    17881 gcaccctgct ttgtttgcta ataaaatttt tgactctttg caatattaat ttcattttcg
    17941 ataaaaacct tccgtaaaac cgttagtttt caagcttctt cttttgatgg atgattttaa
    18001 agcactttat tctattttga acctggctca tca
//
LOCUS       DBNCWK010000059        17410 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.059, whole genome shotgun sequence.
ACCESSION   DBNCWK010000059 DBNCWK010000000
VERSION     DBNCWK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17410
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.059"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(183..1517)
                     /gene="bioA"
                     /locus_tag="AC06IO_001691"
     CDS             complement(183..1517)
                     /gene="bioA"
                     /locus_tag="AC06IO_001691"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HIO9874870.1"
                     /translation="MVNTDQLIAKDLKHIWHPCSQMKDFETFPPIVVNKAQGSYLYTN
                     KGPVIDAISSWWCKSLGHGHPAVIAAIKNQLDCFEHVIGANTTHTAAVELAEKLSEIT
                     GKQHVFFASDGSSAVEIAMKLAIHASQIKGYKNKNQFIALKNGYHGETIGTMSVSDLG
                     LYKTPYASFGVQCHYIDDIPYVSGVKDPLWTNCDAYWNNVVQQLNKICDKVCAILLEP
                     IIQGAGGMLCYSADFLSKISSWAKEKDIYLIADEIMTGIGRTGEWLACDHARIQPDLI
                     CLSKGLTSGSIPFSCVMIDSTLFDLFYDDYHTGKSFLHSHTYSGNPLAIVAALATIKT
                     IEQDNLLMHSQELGKIMFSYLQDIARDSRKLVNVRGVGAVVAADLIESSQARIGFKIY
                     QEAIKQGALIRPIGNTLYWLPPLNTDKEIIGKLAEITLNSIEKAYNKAQGYE"
     gene            1590..2537
                     /gene="bioB"
                     /locus_tag="AC06IO_001692"
     CDS             1590..2537
                     /gene="bioB"
                     /locus_tag="AC06IO_001692"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIO9874871.1"
                     /translation="MSEEKKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFA
                     TLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAA
                     WRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
                     YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPE
                     SVPINRLIPVEGTPLAQAERVEGIELVRTIATARILMPKSAIRLTAGRTEMSDELQAL
                     CYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV"
     gene            2538..3683
                     /locus_tag="AC06IO_001693"
     CDS             2538..3683
                     /locus_tag="AC06IO_001693"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIO9874872.1"
                     /translation="MTLNIKINEYIAQLQEQGLLRTRQILETDKIDYIRFDSNDYLSL
                     TEDRRIAEAYQKGYELYPCGSGASMVLSGYHSSHQTLERAFAEMLRVDDCLLFSSGYC
                     ANLAITALLGRLGTHCFIDKSVHASIYDGLALSRVTYSRYIHNDMNALSALLKSHLGD
                     SVLITEGIFSMSGQIAPLSTISTLCKENGSELFVDEAHAFGVIGPQGMGSVLGHGLTQ
                     NEVPLRVIPFGKAFASQGAVVVGRKDWINALLQTARSFVYSTAISPALCYGLLKTLEI
                     IAAADSRREKLNQLVAQFKELIKSSPLNWLDSDTPIQQLRLGCPHKALHYASELKKDG
                     IYCSAIRAPTVNSASSGLRIIINYKHTIEQIIELFNKLHLIHEYSSR"
     gene            3664..4383
                     /locus_tag="AC06IO_001694"
     CDS             3664..4383
                     /locus_tag="AC06IO_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIO9874873.1"
                     /translation="MNIHLDKHGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVD
                     LPGFGLTPMMDWESFKKNLLGQLPDKFALAGWSMGGLYATRLAIEEPARVQYLINITS
                     SPRFISDIDWPGVAEEVFVNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEG
                     LAFGLEILGTWDFREQLKQISIPTVYLFGRLDPITPAKTMAIMEKNYPNFKYVLFNRA
                     AHMPFLSHTDLFITMMDEFIK"
     gene            4380..5018
                     /gene="bioD"
                     /locus_tag="AC06IO_001695"
     CDS             4380..5018
                     /gene="bioD"
                     /locus_tag="AC06IO_001695"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIO9874874.1"
                     /translation="MKRYFVTGTDTDCGKTFVTNQLVNYFSSSAAIKPIASGCEYSEN
                     QLVNSDALLHQQQNHLPLEVVNPWRFRLPVSPHLSARADGASIDVHKVADYCLNLQLN
                     DIKKLFIEGAGGLMVPLNEQDTWLDFLKLTRIPVILVVGMKLGCINHTLLTQEVLEIN
                     KIKCQGWIANCLDQDMLMLDENISTLEAKLEYPLLARTNYGGKISDICLSSL"
     gene            5153..5467
                     /locus_tag="AC06IO_001696"
     CDS             5153..5467
                     /locus_tag="AC06IO_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIO9874875.1"
                     /translation="MPIYEYQCTNCHHQFDLMQKVTDAPVKQCPKCYENTVTKLISPA
                     GFQLKGTGWYVTDFKNKGNGPKESTENKSGDPGINKENSSKETPAASSADSAKSTKGD
                     SE"
     gene            5464..6093
                     /locus_tag="AC06IO_001697"
     CDS             5464..6093
                     /locus_tag="AC06IO_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIO9874876.1"
                     /translation="MKLISLRAYLITGLIVWLPIFVTIVVLRFIIDMLDSTLALFPKA
                     YQPEQLFGFYIPGFGVLFSLALLLVTGIIATNFFGQRLVSRGESLLAKIPLVRSIYNA
                     VKQVIHAVLSTNSQAFRKVVLVEYPRKGLWTIAFQTGSVNPEIKEKSKEDMMSVFVPT
                     TPNPTSGFMLMIPRQDAIELNMSIDEALKLVISLGVMQSDPALAQVKTQ"
     gene            6112..7893
                     /gene="aspS"
                     /locus_tag="AC06IO_001698"
     CDS             6112..7893
                     /gene="aspS"
                     /locus_tag="AC06IO_001698"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIO9874877.1"
                     /translation="MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGL
                     LQVVYEPENKEIFAIAEKLRSEFVVRVTGIVRKRPEGMINDKMETGRVEVIGTQLEIL
                     NQSPTPPFLPDDHQIINEDLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFL
                     DIETPMLTKATPEGARDYLVPSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCF
                     RDEDLRADRQPEFTQLDIEMAFINEEDILQLIEGLLKIVFKEILNITLPDKLPRMSYK
                     EAMTRYGSDKPDLRNPLELIDIADLVKDCDFNVFASAANDNSGRVVALKLPNGCDLSR
                     KDLDNYGQFVTIYGAKGLAYIKVNDLSAGMAGLQSPILKFLSETAVQSILNRVEAQTG
                     DVIFFGADKAHVVNESMGALRNKLGHDRNLINSGWQLLWVVDWPMFELDPQSNKLQPM
                     HHPFTSPQELSAETLRSKPTQTLAKAYDIVINGYEIGGGSIRIHQPELQKTVFDLIGI
                     DEQEAHEKFGFLLDALQYGAPPHGGIALGIDRLAMLLTDSTSIRDVIAFPKTQTASCP
                     LTSAPSPAGNAQLTELGIRLAPTITTK"
     gene            7868..10819
                     /locus_tag="AC06IO_001699"
     CDS             7868..10819
                     /locus_tag="AC06IO_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HIO9874878.1"
                     /translation="MRQLLRQSNLNLSTTSLKYLWCFFLPLFLLFFSFSLFSASVKEN
                     NATLVEYLAHEKTHLTIAINEIKQKSAPKNEQEYNEKLRQIRTILSMNKAKIESLNSF
                     SENQKKEEVNLNDQLKRLQQMPLANAEVTIQEKVGKIETLLTVNKKTTELIAETLDLA
                     KQYQLLLNDVLNELKLWKANFDLKEKLKSIKAIRAKLNKELNILYENAVSSQDKKQPE
                     LTISEQIQYEANLYINNQRIAVIHQKLNALELQKKIIKTNILLLNNLDTKALQSAIDN
                     YREVINQYSAIESSLQTILNSLTNQSNLFVEPGLQKSVSLLQNDVKTELKETSNQKLI
                     LSKNLEDYQNQLKKQISTRQSLSEYNIDSWAVILKKLIDIPNLFYKYIKTLTIKVYDS
                     YTWLDTFPAIFLWTVLIFILSLFYILNHVLKTLARDKERSRFTGYLYGGILVLLQRNI
                     PHLCFFAMLSFILYFTSISYANSQLLIKLIIVWLTFRVLILIARLILLESISDSSGKD
                     VKLYYRLKWLLLFGGWTTALMTFSHLLPLSILLQDIFNRLFMLFMLTVSLVAWKSKDV
                     IPYLIRPLLKSKKRYFKNAISLLVILVPITLFTTAIIGLIGFVNLAWTMSRYQAYILL
                     VLVGYILVRGLIFDALELFSEWMISSLKNGWLWIEVFLKPIDKILRIVLFLTSVLILF
                     QLFGWYSDSLVVTSLEKVVQYSILNITGIHITVASIIEFLILLAVLVWAAKWTREFCF
                     RWLYKNTKDVGIRHSLSVFTQYAIILLGTFITLHVLGLDFSGMSMILGGLAVGMGFGL
                     RDFASNIVGGIMLLIERPVREGDLITIGEYEGQVAHIGIRSMRVSSWDNTQVLIPNAE
                     TFNKPFTNWTHQDGIIRTVIPIKVSRADDPVMIQQLILDVLAIIPEIVPDPPAQVFLK
                     KIDTALIEFEARYFINIQNHNRVEVRSKVLFAITAQFKAAGIQPPIEPITVELKESSS
                     DFSLKKQTTEE"
     gene            10782..11456
                     /gene="mutH"
                     /locus_tag="AC06IO_001700"
     CDS             10782..11456
                     /gene="mutH"
                     /locus_tag="AC06IO_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HIO9874879.1"
                     /translation="MILALKNKPPKNEKELLEQCQRIEGLSFAQLALELGLFTPVNPV
                     HRKGWVGQALELALGADAKNESLPDFKQLGIELKTIPINKSGKPAESTFIVSIPLLTI
                     HQQSWESSQCYAKLKKVLWIPIEGDTDIPFPHRRIGCGFLWSPNKDQESVLAEDWNYL
                     TNQIVLGQLETLNSSSGEYLQVRPKAANGKSLCYGYDPEGNRIRTLPRGFYLRSCFTS
                     VILSQS"
     gene            complement(11529..12569)
                     /locus_tag="AC06IO_001701"
     CDS             complement(11529..12569)
                     /locus_tag="AC06IO_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874880.1"
                     /translation="MKTIFANKELLYNQYKKMFLEQISDISKLKECIKARYDIKELPD
                     EILFKLLTNNHKKIYDFIGSHNQNRIRYFFGNFDNFDPRDNQISVLDRFILDSVHFYI
                     TSSLLMQLHKEYQENAMEIDKAWQKEIQAHAHFGPLPTIAIIGNLIKSNKNHVFKDLQ
                     FPANKNIQLFQLNKLSIKLNDQVFALWLPKTNETQVSLSNFTRNTIFVCIATAAILAI
                     GVSLFVFSTSSAASLAVIFNPASLGIMIALLACATLMLGISSLYSSTTIHNSTIEHQE
                     NRTPEEPYHSHNIRKDKIHSSKAKPEEQLPYHDLKWLLFNRKPYTLKESIGTPATASE
                     QISSMYTNRQKL"
     gene            complement(12768..13604)
                     /locus_tag="AC06IO_001702"
     CDS             complement(12768..13604)
                     /locus_tag="AC06IO_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874881.1"
                     /translation="MGKVNAVGMAEVGVERAKRKEALRVLTSSPFNLDNKEHTQALEK
                     YIKQFELSVNQMYLYQTMSTALTAWGGSWIVGRFLPIPEFANHLLAACLYLGVSGYIL
                     ESFSMTDFYEQLKEMKVLYNWCLKNNKENYSTDIDNTNNLLYPDIQRMIKLIAPLCST
                     DFMIAWPKEVKTAETQSGILSNTLSIGYSIVSAPFSLFSTASKPSQDQVQRIKDLKVQ
                     VETRGLDLGVFAGFEQAVKYFATNSDFRDLLKAKLTQPVNSFKNLLQTTIPGMLTGSN
                     SK"
     gene            13750..15339
                     /gene="legK1"
                     /locus_tag="AC06IO_001703"
     CDS             13750..15339
                     /gene="legK1"
                     /locus_tag="AC06IO_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector kinase
                     LegK1"
                     /protein_id="HIO9874882.1"
                     /translation="MPRTMFFSVDAANLQNLQITNQKAFAALESYLNDNDSPGAWNNN
                     QGYKYTHKDVTYCFSFNQSLVRRPRKSDQKKHTFEIFDPNKNPLGKGAYGIVYPILGT
                     IQFEFGNAIKRPPKNKLVKIQNHSERDQSYSVLREYQGLLQSGHIAVKPPIFVENNES
                     KFSYLIMEKAEGIVLERFLNPVKRLDLSEEVPELNLINRIEITFAILKAIKEQVTDKH
                     LIHRDIKPGNIIIDFSKSPPIAKVIDFGFVLRASEQDYRRCGTRAYRAPESFNTQALY
                     TAKADVWSTGRILSYLWGDKYTNYYISRDKGLDYVIEKSRNELLFSDPELELYLTKED
                     KSKIRSCLSTMLIVDPEERGSIDKAIKQFSQIDFEKYKRLKRSTNFEIDLTDNDIKLR
                     KQLNSIHLHLIALQSKEKDLRNRACFDAANAMSQLVAKLTTYTNYLEKNPDPFLIKRY
                     KDCCIVEMDLANLTLQNHRDALWLVAELSTAILLLGVGYLFAFGINYYYTGRLGLFSQ
                     TRSEKLVEEMKSSVLGIAVGN"
     gene            complement(15393..16202)
                     /locus_tag="AC06IO_001704"
     CDS             complement(15393..16202)
                     /locus_tag="AC06IO_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1484 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874883.1"
                     /translation="MPSMEMNCCIGKSLNYMRFSMSSFKHSCEKIDINLAHNVLTICP
                     KNREALQRITALFNNLYILYFIGGIPSITYDDMDQIQINIKYSQQITDAKLKNTLLSF
                     VNSELISQDEKETFLNELELTKTSFTTQFNTELENIRKKAEELSKKAEVDPYHYESAS
                     KAANRLHQELLIASQLYFYHKTPEAYENFKQTCQREIERARPVLEQHRGWKPLLVNVG
                     AAIIGLGVLYLLAASINYYKTEGRHFFFHFETDSLQKLENLNMAQSQVLRI"
     gene            complement(16401..16802)
                     /locus_tag="AC06IO_001705"
     CDS             complement(16401..16802)
                     /locus_tag="AC06IO_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874884.1"
                     /translation="MQESIHSLFLAQAIGLYLLIVGIIMLSRASYYRELLTHLKEGSA
                     TIVTAGSLGLIIGICLVLVHNIWVPESEVLVTLVAWLILIKSILWLGFPEFMVKCSQK
                     AYTGTGYYVISIVVLIIGILLITHGFYLFSH"
     gene            complement(16888..17409)
                     /locus_tag="AC06IO_001706"
     CDS             complement(16888..17409)
                     /locus_tag="AC06IO_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIO9874885.1"
                     /translation="MKNKTTKNYPNEKNTEYPIEILDKIDRKILNILQKNNQITNQAL
                     ADIVGISAPPCFRRVKRLREEKIIVNDVALVDPFKVGRPLIVFVNITLEKQREDLLTH
                     FERKIQEEPEVMQCYFVSGDTDYLLIIHVKDMNHYNEFARRVFANEPNIRKFRSSFCL
                     NRTKYNTQVVLDE"
BASE COUNT         5449 a         2967 c         3373 g         5621 t
ORIGIN      
        1 tatttgtcta atttattctc cactcaataa tttaagtaac ttcgataaac tatgttaaat
       61 acacattgta tctcgctgtg tttaattttg ttgtcaacat agtgcccagt ttagattcaa
      121 acgaaaaaag agttaggtta gattggcgaa ccgatttgtc tttaaactat ttgtgaataa
      181 ccttactcat atccttgagc tttgttatag gctttttcta tagagtttaa tgtgatttct
      241 gctaatttcc caatgatttc tttatctgtg ttaagaggtg gtaaccaata gagagtattt
      301 ccaattgggc gaatcagtgc gccttgtttt attgcttcct gataaatttt aaatccaatt
      361 cttgcctgac tgctctctat gagatcagcc gcaacaactg ctccaacacc acgaacattt
      421 acaagttttc tactgtccct tgctatatcc tgcagatagg aaaacatgat tttaccaagt
      481 tcctgactat gcatgagtaa attatcttgt tctattgttt taatagttgc taaagcagcg
      541 actatagcaa gtggattacc gctataggta tgtgagtgca aaaaagattt ccctgtatgg
      601 taatcatcat aaaaaagatc aaataaggta ctgtcaatca tgacacagct aaatggtatt
      661 gagccggaag ttaatccttt tgatagacaa atcagatcag gttgtatcct tgcatgatca
      721 caggctagcc attctccagt tcttcctata ccagtcataa tctcatcagc aattaaataa
      781 atgtcttttt ccttagccca tgacgagatt tttgagagaa aatctgcact gtaacatagc
      841 atacctccag caccctgaat gataggctcc agaagtatcg cgcaaacttt atcgcaaatt
      901 ttattcaatt gttgtacgac gttattccaa taagcatcgc aatttgtcca taagggatcc
      961 ttaacgcctg agacataggg aatatcatcg atgtaatggc attgcacgcc aaacgatgca
     1021 tagggagtct tatagagtcc caaatcactg acactcatgg tgccaatagt ttctccgtgg
     1081 tagccatttt ttaaggcaat gaattggttt ttatttttgt acccttttat ttgtgaagca
     1141 tgaatagcta atttcatggc aatttcaaca gcactggaac catcactggc aaaaaacaca
     1201 tgctgttttc ctgtaatttc gcttaatttt tcagctaatt ccactgcagc agtatgagtt
     1261 gtattggcac caatcacatg ctcaaaacag tccagttggt tcttgatagc agctatcact
     1321 gctggatgac catgccctaa tgatttgcac caccaactgg aaattgcatc aatcacaggg
     1381 cccttattgg tatagagata gctaccttgt gctttgttga caacgatagg ggggaatgtt
     1441 tcgaaatctt tcatttgtga acaaggatgc caaatgtgct ttaaatcttt tgcaatcagt
     1501 tgatctgtgt ttaccatttt aaaaattttg gttgacaagt ataggcgcga ctttatcatg
     1561 ttcactattc tgaattaaag ggggtagccg tgtctgaaga aaaaaaacag tggtcaattt
     1621 ctgatattga agcaatttat caacaaccat ttaatgattt actttatcaa gcacatacta
     1681 ttcataggac atatcacgat cctaattctt tacaatttgc aaccttgtta agcattaaaa
     1741 cgggcgcatg cccagaagat tgtggctatt gctctcaaag tggtcactat aaaacgcatg
     1801 tagaaaaaga aaaattgatg tcagttgaag aggtattgca atgtgccaaa gaagcaaagg
     1861 agggtggggc caaacgtttt tgtatgggag ctgcatggcg ttgtccacct gataaagcaa
     1921 ttccgcagct aaaagaaatg atagagggag taaagtcttt agggcttgaa acctgtatga
     1981 ctttaggcat gcttaccaaa gaacaggcct ctcatttaaa ggaggctgga ttggattatt
     2041 ataaccataa tattgatacc tcaccatcct attacgataa ggtggtaacc actcgaaaat
     2101 tcagtgatcg attagatact ttaaataacg ttcgttcagc gggaattaat gtatgttgtg
     2161 gtggtatttt aggattgggg gaaaccagag aagatcgaat cgaattttta ttaacattag
     2221 ctaacatgga aactcctccg gaaagtgtgc caattaatcg tttaattcca gtggaaggaa
     2281 cacctttagc ccaagctgag agagtagaag ggattgaatt ggtaagaacc attgctacag
     2341 caagaatttt aatgcctaaa agtgctatta gattgacagc cggtcgaacc gaaatgagcg
     2401 acgagttaca agcactttgc tattttgccg gagccaattc tgtgtttatt ggcgataaat
     2461 tattgacaga agataatccc cagcgcttca aagacaaaac tctttttaac aaactgggtt
     2521 tgactgagat ggtataaatg actcttaata taaaaattaa tgagtacata gcacagctgc
     2581 aagagcaagg attattgcgt acaagacaaa ttttagaaac ggataagata gattatattc
     2641 gatttgactc taatgattat ttgtcgctca cagaagatag gcgaattgct gaagcctatc
     2701 aaaagggata tgagttgtat ccttgtggaa gtggtgcttc gatggtttta agtggctacc
     2761 atagtagcca ccaaaccctt gagagggcct ttgccgaaat gctacgagta gatgattgtc
     2821 ttttattttc ctctgggtac tgtgcaaatt tggctattac ggccttgctt gggcgtttgg
     2881 gaacacattg ttttatagat aaaagcgttc atgcatcaat ttatgatgga ctggctttgt
     2941 cacgagtaac ctactctcgt tacatacata atgacatgaa tgctttgtct gccttgttaa
     3001 aatctcattt gggcgattct gttttgatta cagaaggtat ttttagtatg agtgggcaga
     3061 tagcgccgtt atcaacgatt agtacgttat gcaaagagaa tggctctgag ctctttgtag
     3121 atgaagcaca tgcttttggt gtgattggac cgcaaggaat gggctctgtg ctaggccatg
     3181 ggctgacaca gaatgaagtt cccttgcgag ttattccttt tggtaaggca tttgcttccc
     3241 agggcgcagt ggttgttggg cggaaagatt ggataaatgc tttgttgcaa acggcacgtt
     3301 cttttgttta ttcaactgct attagtcctg ctttatgcta tggtttgctc aaaacattgg
     3361 agataatagc agctgccgac tccagaagag aaaaattaaa ccaattagtt gctcaattca
     3421 aagagttaat aaagtcttcg cccttaaatt ggctggattc agatactcca attcagcaat
     3481 taaggttagg atgtcctcat aaggcattgc attatgccag tgaactgaaa aaagatggga
     3541 tttattgctc tgctatcaga gctccaacgg taaatagtgc ttcttccggt ttacgaatta
     3601 ttattaatta taagcatact attgaacaaa tcattgagtt atttaataaa ttgcacttaa
     3661 tacatgaata ttcatctcga taaacatggg caaggaatgc ctttggtcct atttcatggc
     3721 tggggatttg atagccaaat ctggcaaccg ataattcctt accttaagcc taaatatcag
     3781 attattttgg tcgatttacc tggttttgga ttgactccaa tgatggactg ggaaagtttt
     3841 aaaaagaatt tgcttggcca attacctgat aaatttgcat tggcagggtg gtcaatgggt
     3901 gggctgtacg cgacacgttt agcaattgaa gagcctgcca gagtgcagta tttgataaat
     3961 ataacgtctt cacctcgctt tatctctgat attgactggc cgggagtagc agaggaggta
     4021 tttgtcaatt tttataacaa tttatcgaaa gatattaata agacattaaa agagttcata
     4081 tccttgcaac tcaataagat gaaatttgat tttaaaatag gtaatcctcc atctccagaa
     4141 ggcttggcgt ttggtctcga aattttgggt acctgggatt ttagagagca gttgaaacaa
     4201 atatctatac ctactgttta cttatttggt cgacttgatc cgattactcc tgccaagacc
     4261 atggcaatta tggagaaaaa ttacccgaat tttaagtatg tcctcttcaa cagagcagct
     4321 cacatgcctt ttctgtccca taccgattta tttattacaa tgatggatga atttataaaa
     4381 tgaaacgata ttttgttacg ggtactgaca ctgattgtgg taagacattt gtaaccaatc
     4441 aattagttaa ttatttttca agttcggcag cgataaaacc tatagcaagc ggatgtgagt
     4501 acagtgaaaa tcaattagta aattctgatg cacttttaca ccagcaacag aatcatttgc
     4561 ctttggaagt tgttaatccc tggcgattta gattgccagt ttcgccgcac ttatcagcaa
     4621 gggcggatgg tgcaagtatt gatgtccata aagtagctga ttattgcctt aatttgcaac
     4681 tgaatgatat taagaaatta tttatcgaag gagccggggg gttaatggtt cctctgaatg
     4741 agcaggacac ttggcttgat tttttaaaat taaccaggat ccctgttatt ttagttgtgg
     4801 gcatgaagtt gggttgcatc aaccacactt tgcttaccca ggaagtgctc gaaataaata
     4861 aaatcaaatg tcaaggatgg attgctaatt gcctggatca ggacatgcta atgcttgatg
     4921 aaaatattag tacccttgaa gccaaattag aatatccttt attagccagg acaaactatg
     4981 gcggcaagat ttctgatata tgtttaagct ccttgtaatc gcagtgaaat tttatatcaa
     5041 aacaacacta tatacctgat gttattgctt gttctgcttg cgctataccc atggcagcaa
     5101 ttataatgaa aagttaaatt acagatactt cgagtttatg gagtgatgct agatgccaat
     5161 ttatgaatat cagtgcacaa actgtcatca tcaatttgat ttgatgcaaa aagttacgga
     5221 tgcgcctgta aaacaatgcc caaagtgtta tgaaaatact gtaacaaaac ttatttctcc
     5281 ggctggattt cagttaaagg ggactggctg gtatgtcaca gattttaaaa ataaaggtaa
     5341 cggaccaaag gagtcaactg agaataaatc aggagatccc ggcatcaata aagagaattc
     5401 ttctaaagaa actcccgcag caagctctgc agatagcgca aaatcgacaa aaggtgatag
     5461 cgagtgaaat taatctccct cagagcctat ttaattaccg ggttaatagt gtggttacca
     5521 atatttgtaa ccattgtagt cctgcgattt attattgata tgcttgatag caccttggct
     5581 ttatttccaa aagcttatca acctgagcag ttattcggtt tctacatacc aggctttggc
     5641 gttttatttt cattggcttt attactcgtg acaggaatta ttgcaaccaa tttttttggg
     5701 cagagacttg taagtcgggg tgaatcctta ttggctaaaa tacctctggt gcggtcaatt
     5761 tataatgcag taaagcaagt tattcatgct gttctatcaa ctaatagcca agctttccgc
     5821 aaagttgttt tagttgagta tcctcgtaaa ggactttgga ccatcgcatt tcagactggc
     5881 tccgttaatc ctgaaattaa agaaaaatcc aaggaagaca tgatgtctgt atttgttcca
     5941 actacaccta accctacttc gggttttatg ttgatgatac ccaggcaaga tgccatcgag
     6001 ttgaatatga gcattgatga agcgttaaaa ttagtaatat cgttaggtgt aatgcagtct
     6061 gatcctgcat tggcacaagt aaaaactcaa taaactttaa taggtggttt tatgcgtaca
     6121 cactactgtt cggccgtgaa tgaattaagc ttggataagc aaatcacagt ttgtgggtgg
     6181 gtgcataata gacgagatca tggcggagtt atttttttag acattcgtga tcgttccgga
     6241 ttattgcaag tggtgtatga gcctgaaaat aaggaaattt ttgcaattgc tgaaaaacta
     6301 agatcagagt ttgttgttcg tgttactggc atagtccgta aaagaccaga aggaatgatc
     6361 aatgataaaa tggagactgg cagggttgaa gtaatcggta cacaacttga aattcttaat
     6421 caatctccaa ctccaccgtt tttgccagat gatcatcaaa ttatcaatga agatttgcgc
     6481 tataaatacc gctatattga tttgcggcgt gctgttatgc agaagaagct gactttaaga
     6541 cacaaattaa acagttgcat acgtaattac ttgaatgagc agaatttttt agacatagaa
     6601 acgcctatgt taactaaggc aactcccgag ggcgcgcgag attatcttgt gccttcaagg
     6661 gtgcatccag ggcagttcta tgctttacct caatcgccac aattatttaa gcaattgttg
     6721 atgatgtcag ggtttgataa atattaccag attgtacgct gttttagaga cgaagattta
     6781 agggctgaca gacagccgga atttacgcag cttgatatag aaatggcatt tattaatgaa
     6841 gaagatatat tgcaactaat tgaaggattg ctcaagattg tatttaagga aatccttaac
     6901 attaccttac ctgataaatt gccaagaatg tcgtataaag aggctatgac tcgctatgga
     6961 agtgataaac ctgatctgcg taatccactg gagttgattg atattgcaga tttggtaaaa
     7021 gattgcgact ttaatgtatt tgcttctgct gcgaatgata attcaggaag agtggttgct
     7081 ttaaaattac ctaatggatg tgatcttagt cgaaaggatc tggataacta tggacaattc
     7141 gtaactatat atggggctaa aggacttgca tatattaaag tcaacgattt aagtgctggt
     7201 atggcgggtt tgcagtcacc aattctaaag tttttatctg aaacggctgt gcaatctatt
     7261 cttaatcggg ttgaggcgca gactggcgac gtcatttttt tcggtgctga taaagctcat
     7321 gtagtaaacg aatctatggg agccttaagg aataaactgg gtcatgatag aaatctgatt
     7381 aattctggat ggcaattact gtgggttgta gattggccta tgtttgaatt ggatcctcag
     7441 agcaataagt tacagcctat gcatcatccc tttacatccc ctcaggaact atctgccgag
     7501 acacttcgtt ccaagccaac gcaaacattg gccaaggcat atgatatagt tattaatggt
     7561 tatgaaattg gtggtggatc tattcgtatc catcaacctg aactacaaaa aactgtattt
     7621 gatttgatcg gaattgatga gcaagaggca catgagaaat ttggattttt actcgatgcc
     7681 ttacaatatg gtgctccacc tcatggtgga attgcgttgg gaattgatag attagctatg
     7741 ctgctgacag actcaacttc cataagggat gtgattgcgt ttcccaaaac tcaaacagca
     7801 tcctgtcctt taacaagtgc gccatcgcct gcgggcaatg cacaactaac tgaactgggt
     7861 ataagacttg cgccaactat tacgacaaag taatttaaat ttaagcacca caagtttaaa
     7921 atacttgtgg tgctttttcc tgcctctgtt tttattattt tttagttttt cactcttttc
     7981 agcatcggtt aaagaaaaca atgccacact agtagaatat ttggcacatg aaaaaactca
     8041 tttgaccatt gctatcaatg aaattaaaca aaagagcgct cctaaaaatg agcaagagta
     8101 caatgagaag ttaagacaaa ttagaactat tctttcaatg aataaagcaa aaattgagag
     8161 tttgaatagt tttagtgaaa atcaaaagaa agaagaagtc aatttgaatg accaactaaa
     8221 gcgtttgcaa caaatgccgc ttgccaatgc tgaagtaact attcaggaaa aagtaggtaa
     8281 aatcgagacc ttgcttactg tcaataaaaa aaccactgag ttaattgcag aaactttgga
     8341 tttagccaaa caatatcaat tacttttaaa tgatgttttg aatgaattaa aactttggaa
     8401 agccaacttc gatttgaaag aaaagcttaa gtcaattaaa gccattagag ctaaattgaa
     8461 taaagaactg aatatccttt acgagaatgc agtatccagc caagataaaa agcaaccgga
     8521 actaacgatt agcgaacaga ttcaatatga agctaatttg tacatcaata atcaaagaat
     8581 tgcagttatt catcaaaagt tgaatgcatt agagttacag aaaaagataa ttaaaacgaa
     8641 tatattgtta ttaaataact tggatactaa agccttgcaa tcagcgattg ataattacag
     8701 agaggttatt aatcaatatt cggctataga gagttctttg caaacaattc tgaattctct
     8761 tacaaatcag tccaatcttt ttgttgagcc agggttacaa aaatccgtta gcttattgca
     8821 gaatgacgtt aaaacagaat taaaggagac ctctaatcaa aaacttatct tatccaaaaa
     8881 tttagaggat tatcaaaatc aattaaagaa acagatttca acaagacaaa gcttaagtga
     8941 atacaatata gacagttggg cagtcattct caagaagcta attgatatcc ccaatttgtt
     9001 ttataaatac atcaaaacac taaccataaa ggtttatgat agttatacct ggctagatac
     9061 tttccctgct atttttttat ggacagttct tatttttatc ctgagcctgt tttatatttt
     9121 aaatcatgtt ttgaagactt tagcaagaga taaagaacgc tcaagattta ctggttatct
     9181 ttatggtgga atactcgttt tactgcaaag aaacatacct catctttgct tcttcgctat
     9241 gttatctttc attttatact tcaccagtat ttcttatgct aactctcaat tattaattaa
     9301 actcattatc gtgtggctta cctttagagt tttaatttta atagcccgtt tgattttgtt
     9361 agaaagtata tcggattcat caggtaagga cgttaaatta tattatcgac taaaatggtt
     9421 gttgcttttt ggtggatgga caaccgcgtt gatgacattt agtcacctgt tacctttatc
     9481 aattctactg caagatatat ttaatcgatt attcatgctt tttatgttaa cggtatcctt
     9541 ggtcgcatgg aaaagtaaag atgtaattcc ttatctcatt agaccattac tgaaatcaaa
     9601 gaaaagatat tttaaaaatg caatttcttt attggtcatt ttagttccta taaccttatt
     9661 tacaacagca attattggtt taattggctt tgtgaatctg gcatggacta tgagtcgtta
     9721 tcaagcttat attctgttgg ttttagtagg ttatattctc gtaagaggtt tgatatttga
     9781 cgcattagag cttttctctg agtggatgat ctcttcattg aaaaatggtt ggttatggat
     9841 agaggttttc ctcaaaccaa tcgataaaat cttgcgtatt gtcttgtttt tgacaagtgt
     9901 attaatttta tttcagttgt ttggttggta ctctgattca ctggttgtaa ctagcctcga
     9961 aaaagtagtt caatacagta ttcttaatat tacgggcata catattacag tcgccagtat
    10021 cattgaattt cttattctgc ttgctgtttt agtatgggca gcaaagtgga caagagaatt
    10081 ttgctttcga tggctatata agaataccaa agatgtgggt atacgccata gtttatctgt
    10141 atttacacag tatgcgatca tattactggg taccttcatc actcttcatg ttctgggact
    10201 ggattttagc ggtatgtcga tgatacttgg aggacttgct gtcggtatgg gtttcggttt
    10261 aagagatttt gcttcaaata ttgttggagg cattatgctg ttaattgaaa gacccgtaag
    10321 ggaaggcgat ttgatcacca taggtgaata cgaagggcaa gtggctcaca taggcattcg
    10381 ctcgatgcgc gtatcttcat gggataatac tcaggttttg attccgaatg ctgaaacatt
    10441 caataaacct tttacaaact ggactcatca agatggtatt attcgtacgg tcataccaat
    10501 aaaggtaagc agagccgatg atccagtgat gattcaacag ttaatacttg atgtattggc
    10561 aattattccg gaaattgttc cagatcctcc cgcacaagtt tttttaaaga aaatcgatac
    10621 agctttaata gaatttgaag cacggtattt tattaacatc cagaatcata accgggttga
    10681 agtaaggtca aaagtcttgt ttgccataac tgcacagttt aaagcggcag gaatacagcc
    10741 gccaattgag ccgattactg ttgaactaaa agagagctcc agtgatttta gccttaaaaa
    10801 acaaaccacc gaagaatgaa aaagaacttt tggagcaatg ccaacgaatt gaggggttga
    10861 gtttcgctca gttagctctt gaattaggtc tatttacccc tgtaaaccct gtgcatcgaa
    10921 aaggatgggt tggacaagct ctcgagttag ctttaggtgc tgatgcaaaa aatgagtcgc
    10981 tacctgattt caagcagttg ggcattgaat taaaaacgat acctattaat aaatcaggga
    11041 aaccggctga gtccacattt attgtttcta ttccactact gaccattcat caacaaagtt
    11101 gggagtcttc tcaatgttac gctaaattaa aaaaggtatt atggattcct atagagggag
    11161 atacagatat tccctttcct cacagaagaa ttggatgtgg gtttttatgg tctccgaata
    11221 aagatcagga atctgttctt gcagaagatt ggaattattt aacaaatcaa atagttctgg
    11281 gacaattaga aacattaaat tcttcttcgg gagagtatct tcaagtgagg cctaaggctg
    11341 ccaatggaaa atctttatgt tatggttatg acccagaggg taatcgtata agaacgttac
    11401 cacgaggatt ttatttacgc tcctgtttta catccgtaat tttaagtcaa tcctaatttt
    11461 aaggataaat taagcaccat ctgtttacca gtattatgat aaattttaca gatattaggg
    11521 acagctgatt atagcttttg tctattagta tacatggacg atatttgttc agatgctgtt
    11581 gctggagtcc ctattgattc ttttaatgta tatggttttc tgttaaataa taaccatttg
    11641 agatcatggt agggtaactg ctcttctggt ttggccttac tagaatgtat tttatccttg
    11701 cgaatattat gagagtgata aggttcttct ggtgttctgt tctcctgatg ttctattgtg
    11761 ctgttatgta tggtagtgga agaataaaga gagcttatac ctagcattag cgtggcacat
    11821 gctaacaatg caatcattat tcctagtgat gcaggattaa aaataacagc taaactagct
    11881 gctgagcttg tagaaaatac aaagagactt acgcctattg ctaaaatagc ggcggtggca
    11941 atacaaacaa aaatggtatt gcgagtgaag ttggatagtg atacttgtgt ttcatttgtt
    12001 ttgggtaacc agagcgcaaa gacttgatca ttaagcttaa ttgataattt atttaattga
    12061 aagagctgta tgtttttgtt cgcagggaat tgtaaatctt taaagacgtg gtttttattt
    12121 gatttaatca agtttccaat gattgctatt gttggtaatg gaccgaaatg ggcatgggct
    12181 tgaatttcct tttgccatgc tttatcaatt tccattgcat tctcttgata ttctttgtgt
    12241 aactgcatta ataaactgct agtgatataa aaatgaacgc tatcaagaat aaatcgatct
    12301 aaaacgctaa tttggttatc acgtggatca aaattatcaa aattaccgaa aaaatatcgg
    12361 attctatttt gattatgtga ccctatgaaa tcgtaaattt ttttatgatt attagttaat
    12421 aatttaaaaa gtatttcgtc aggaagctct tttatatcat accttgcttt aatacattct
    12481 tttaatttgc tgatgtcaga tatctgctct agaaacattt ttttgtattg attgtaaagc
    12541 aattccttgt ttgcaaaaat agttttcatc ttgagaaacc ttcgtggagt aaatcaatga
    12601 cattatttgc tgttaatcat cctcatattg gccttaaaga tgattatgcc tcctttcttt
    12661 aatgctaatg ccaaccttta ataaggctta aggtcggcaa gaaaaacatc agctctttac
    12721 ctaatacgat cgttaggtaa tatttaagca tgagggatct attaccatta ttttgaatta
    12781 gatcctgtca acattcctgg tatagttgtt tgcaacaaat ttttaaatga gttgacaggc
    12841 tgagttagct tggctttcaa tagatctcta aaatcagagt tcgtagcgaa atacttgact
    12901 gcctgctcaa aaccagcaaa aacccctaag tcaaggcctc ttgtttctac ctgcactttt
    12961 aggtctttaa ttcgttgcac ttgatcctga ctcggtttac tggctgttga gaacagtgaa
    13021 aatggcgctg atactataga atagcctata gacaatgtgt tggataggat tccactttga
    13081 gtttctgcgg tttttacttc tttaggccag gcaatcataa aatcagtaga gcataaaggg
    13141 gcgattaatt taatcatgcg ctggatatca ggatacaaaa ggttattggt attatcgata
    13201 tcggtactat agttttcttt gttatttttt agacaccaat tatagagtac tttcatctct
    13261 ttcaattgtt cataaaaatc ggtcatgcta aatgattcaa gaatgtaacc agaaacgcct
    13321 aaatagagac aggctgcaag aaggtgattc gcaaattcag gaataggcaa aaaccttcca
    13381 actatccacg agccccccca agctgttaat gcagtactca ttgtttgata gagatacatt
    13441 tgattgacac ttaactcaaa ttgtttgatg tacttttcca atgcctgagt gtgttcctta
    13501 ttatccaggt tgaagggaga tgacgtcaga actcttaggg cttctttcct tttggctctt
    13561 tcaactccga cttcagccat tccaactgcg tttaccttac ccacaattct ctccttgaaa
    13621 tcaaaatttc attttatact ataaatttaa aaaataatga attagatttt agctaagagt
    13681 tcaattaatt taacttaata aaaaagacga ggtataataa aaccgatcaa tcaaattgaa
    13741 gtcaatatca tgcctcgtac aatgtttttt tccgttgatg ccgctaatct ccaaaattta
    13801 caaattacaa atcaaaaagc ttttgcagcg ttggagagtt atctgaatga taatgattca
    13861 ccaggtgcct ggaataataa tcaaggatac aaatatactc ataaggatgt tacttattgc
    13921 ttctcattta atcaatcctt agtgagacgc ccaagaaaat cggatcaaaa aaaacataca
    13981 tttgaaattt ttgaccctaa taagaaccct ttaggcaagg gagcgtacgg tattgtgtat
    14041 cccattttag gtacaattca gtttgaattt ggaaatgcaa ttaaaagacc accaaagaat
    14101 aaattagtta aaatacaaaa ccatagtgaa cgcgatcaaa gttattctgt actacgcgaa
    14161 tatcaagggc ttcttcaatc agggcatata gcggttaaac cgccaatatt tgtagaaaac
    14221 aatgagagta aattcagtta cttgattatg gaaaaagcag agggtatcgt tttagagagg
    14281 tttctaaatc ccgttaagcg gcttgatttg tctgaagagg tacctgagct taatcttatt
    14341 aatcggattg agattacttt tgccatttta aaagcaatta aagagcaggt gacggataag
    14401 catttgattc atcgagatat aaaaccagga aatattataa tcgattttag caagtcgcct
    14461 ccgatagcta aagtaatcga ttttggtttt gtgcttagag cgtcagaaca ggattatcgt
    14521 cgttgcggaa cccgagcata cagagcgcct gaatccttca atacccaggc attgtacaca
    14581 gcaaaagctg atgtttggtc aacaggcagg atattaagtt atttgtgggg agataaatat
    14641 actaattatt atatctctcg tgataagggt ttggactacg tgattgagaa gtcaagaaac
    14701 gaactactct tctctgaccc agaattggaa ctctatttaa ccaaggaaga taaaagtaaa
    14761 atcagatcgt gcctaagcac aatgcttata gttgaccctg aggaaagagg atctattgat
    14821 aaggctatca agcagttttc ccaaattgat tttgaaaaat ataaaagatt aaagcgctct
    14881 acaaattttg aaattgattt aacagataat gatattaaat taagaaaaca gctcaattca
    14941 attcacctgc atttgattgc cctacagagc aaagaaaagg atttgcgtaa tagagcatgc
    15001 tttgatgcag caaatgcaat gagtcaattg gtagccaagc tcactaccta caccaattat
    15061 cttgaaaaaa atccagatcc atttttaata aaacgttata aagactgctg catcgtagaa
    15121 atggatttgg ctaatttaac tttgcaaaat caccgagatg ccttatggtt agtggctgag
    15181 ttatcaactg ctatcctttt gcttggtgta gggtatttat ttgcttttgg tataaattat
    15241 tactacacag gtcgcctggg attattttct caaaccaggt ctgagaaatt ggtggaggaa
    15301 atgaaaagct cggtattggg tattgcggtt ggaaattaaa aatctgtatt tcccggatta
    15361 taacagaatc gtctaatccg ggttgttcgt gtttagattc ttaacacttg agattgagcc
    15421 atattcaggt tttctaattt ttgcaaggag tccgtttcaa aatgaaagaa aaagtgacga
    15481 ccttctgttt tataatagtt aatggaagct gcgagcaaat agagaactcc aaggcctatg
    15541 atggccgctc ctacattaac taacaaaggt ttccatcctc tatgctgttc cagtacaggt
    15601 cgagctctct caatttctct ttgacatgtt tgcttgaaat tttcgtatgc ctcgggtgtt
    15661 ttatgataaa aatacagttg ggaggctatt aataattcct gatgcagtct gttagctgct
    15721 tttgacgcag actcataatg ataaggatca acttctgctt ttttgcttaa ttcctcagcc
    15781 ttttttctga tattttccaa ttcagtatta aattgggtag tgaatgaagt tttggtgagc
    15841 tccaactcat ttaaaaatgt ttctttttca tcttgagaga ttaattcaga attaacaaaa
    15901 gaaagtaatg tatttttgag tttggcatcg gttatttgtt gagaatactt aatattaatt
    15961 tgaatctgat ccatgtcatc ataggtgatg ctagggatac ctcctataaa ataaagaatg
    16021 tacagattat tgaaaagagc tgtaattctt tgcagcgcct ctctattttt aggacaaatg
    16081 gttagaacat tatgagctag attgatatca attttttcac aagaatgttt aaacgatgac
    16141 atactaaacc tcatataatt cagagatttc cctatgcaac aattcatttc cattgaaggc
    16201 aggtcaagta tatgtagaca agcttaagat tttattaaga agcgccttaa atttaacaat
    16261 ttttcaaaaa aactttacga ttttttgaaa tgcaaaaaat atcgttaaac cttgcagatt
    16321 tcctttataa aaaactacac acctcatcca cttgatggtt accttgctga aagcagagct
    16381 gcctatgaaa gcaggtaaaa tcaatgggaa aacaggtaaa atccgtgggt tattagcaga
    16441 atcccaataa ttaataccac tatggagatc acataataac cagtccctgt ataggctttt
    16501 tggctgcatt tcaccataaa ctcgggaaag ccgagccata atattgattt aatcaaaata
    16561 agccaggcta ccagtgtaac taatacctct gattcaggaa cccaaatgtt atgaaccagt
    16621 acaaggcaaa taccgataat taaccccaag cttccagccg ttacaatagt ggcactgcct
    16681 tctttgagat gagtcaacaa ttcacgataa tatgaagctc gagaaagcat tataattccc
    16741 acaataagaa gatagaggcc gattgcttgt gccaaaaata aagagtgaat tgattcttgc
    16801 atggttggct ccttctgatt ccgctcctaa attaattata gtacatgcgg gattgggttt
    16861 gttaactagg caggtacttg ctagttacta ctcatccaaa acaacttgtg tattatattt
    16921 ggttctatta agacaaaagc tgcttctgaa ttttctaata ttgggctcat tagcgaatac
    16981 tctcctggca aattcattgt aatgattcat atctttgaca tgaataataa gcaggtaatc
    17041 tgtgtctcca gagacaaagt agcattgcat aacctcaggc tcttcctgta tttttctttc
    17101 aaaatgagtt agcagatctt ctctttgttt ttcaagagta atattgacga acacaattaa
    17161 tggtctgcct actttaaatg gatcaaccaa agcaacatca ttaacgataa ttttttcctc
    17221 tctcaggcgt ttgactcttc gaaaacaggg tggggcagag attccaacta tatctgccaa
    17281 tgcctggtta gtaatttgat tatttttctg aagaatattt agaatttttc tatcaatttt
    17341 atcaagtatt tcaattggat attctgtatt tttttcattt ggataatttt ttgttgtctt
    17401 gtttttcata
//
LOCUS       DBNCWK010000060        17106 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.060, whole genome shotgun sequence.
ACCESSION   DBNCWK010000060 DBNCWK010000000
VERSION     DBNCWK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17106
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.060"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..1409)
                     /gene="ankI"
                     /locus_tag="AC06IO_001707"
     CDS             complement(<1..1409)
                     /gene="ankI"
                     /locus_tag="AC06IO_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkI/LegAS4"
                     /protein_id="HIO9874886.1"
                     /translation="MIILYDFLQESSVMPRSKNDSKLKKKSALQSKFKEQQLNHGSKE
                     HKSKFKFSQRKAKKKGPGMTHLPGNIYTLFTPVNGPESNHELIATSKIMVDLHIDNMS
                     GNDYIPSAIDRTDLVIVQPVHLLRKTGGRGLFAREDIPKGTCIGIYTGEVYSEQEFEQ
                     YLMEHVGSDKSYAMYVGGRVVDAARKGNLTRYINFSDSQDNAEFVEGTLNRKKVVKVI
                     TTKNIKAGQQLLINYNTYEEQASRYYYFLNPGDGWLSAQEFYQTHQSQYRLEQMPYNL
                     EGFDLKAGDRVLMTQIGRIIFANYSLAKEQELNVSDIDLPFLKVGSDEKILDFDEADT
                     FTPLMVACYLGQVENVKWLIEHGANIDQQQSHSGHCPLSLTLKGYSLAKDTQKYIDII
                     QLLIKNQVNLLVHDRSDKTFLHNAALVLNNLDFQSVVKFLIGQNPIDINEYFTYIDEN
                     DFDIVMHCYNNKLFDKALVL"
     gene            1553..2317
                     /gene="map"
                     /locus_tag="AC06IO_001708"
     CDS             1553..2317
                     /gene="map"
                     /locus_tag="AC06IO_001708"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIO9874887.1"
                     /translation="MGVTIKTPDEIEKMRVAGRLAAEVLEMIGPYVQVGITTDELNTI
                     CHDYIVNVQKAIPAPLNYNGFPKSICTSINHVVCHGIPGKKTLKDGDIINIDVTVIKN
                     EYHGDTSKMFIVGTPSVKAKHVVQIAHECLYIGIDMVKPGVQLGDIGHAIQQHAEKNR
                     CSVVRDYCGHGIGRVFHEDPQVLHYGVPGTGMRLEPGMTFTIEPMVNIGKHHTRLLPD
                     HWTVVTKDHSLSAQWEHTLLVTDTGVEILTLRNEER"
     gene            2304..4889
                     /gene="glnD"
                     /locus_tag="AC06IO_001709"
     CDS             2304..4889
                     /gene="glnD"
                     /locus_tag="AC06IO_001709"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIO9874888.1"
                     /translation="MKNDNRIIKNTIKQFKEKLCRDISQKANITSITRKLAVFIDTIL
                     IQLFIKNKLHFGDNFCLLALGSYGRRELQLHSDIDLLILHTEKVSNIQLQRAQKFIQD
                     CWDVGLEVSHQITTVSSCANLASQDLSVISTIMDMFLLCGHGALMEELIYQTHTLHMW
                     PSHQYFFAKLQEQQSRYAKYGETAYNLEPNIKNGPGGLRDLQILLSISKRHFKIKKLA
                     EGIGYGFITDKEYEELKYCQNFLWRVRFALHMLAGKPEERLSFDYQVKLAQFFGYQDQ
                     SHILAIEQFMKDYFKVIKRNRELNEMLLQWFNETIVYHQKQKIIRLDDEFQLSNRFIE
                     VRNNRVFKQNPQSILKLFYWLVKRPDIEGVRASTIRLIRESLFLMGKRFRESKETANI
                     FVNIFRTGNDPYDALQRMNRYGVLAHYLDCFATVTGQMQYDLFHAYTVDQHTLFVIRN
                     ISRFKKNEYAKQFPLCAKIITALEKPEILYLGALFHDIAKGRGGDHSELGAIEAQQFT
                     QRHYMETEDSKLIVWLVRYHLLMSQTAQRKDIYDPKTIEQFCQLLPHAKYLDYLYLLT
                     VADICGTNPTLWNAWKDSLLKELYHAAKTRLHKQQELLDEAALISIRKQYAMDILISD
                     GISSRVIQDLWNQFKGKYFLHESPEVIARHTKAILNSKQFPVVIIMPHHSQGGTEVFI
                     YMPHKDERFTITTSVLSNHHVTIQEAAIITCDNQFDLDTYIILDENNQAFLNEQRARD
                     IQKSLCDHLANTGRLPAVSRRRLSRALTHFNVKTQINFIDDNTNHQTQLFLVTNDRPG
                     LLATISRVFLTLNIHLHNAKIATAGERVEDMFYISNQTGYSLNHEEKTILKEKLILEI
                     SKSKY"
     gene            complement(4911..5354)
                     /gene="tadA"
                     /locus_tag="AC06IO_001710"
     CDS             complement(4911..5354)
                     /gene="tadA"
                     /locus_tag="AC06IO_001710"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIO9874889.1"
                     /translation="MIDKFWMQLAYEQAVIARNEGEVPVGAVLVSKDNQLLGVGRNVI
                     EKSHDPSDHAEIRAIRQASRKLNNHRLLDTTLYVTLEPCVMCAGLMVHARIKRLVFAT
                     RDFKAGAAGSMFNVLHALNHSVLIDEGIMQAECSQLLSDFFRKLR"
     gene            complement(5351..6928)
                     /gene="guaA"
                     /locus_tag="AC06IO_001711"
     CDS             complement(5351..6928)
                     /gene="guaA"
                     /locus_tag="AC06IO_001711"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIO9874890.1"
                     /translation="MNDLKKSPLLILDFGSQYTQLIARRVREMGVYCEIYPYNINHEQ
                     FKKLNPCGVILSGGPSTVTHDANPRAPQWLFESDLPLLGICYGMQTMAVQLGGQVHSS
                     TLREFGYAELRLHGHSQLLSNIEDRTAIDGSALLDVWMSHGDKVTELPPGFKVICETR
                     NAPIAGMADESRQMYGLQFHPEVTHTLQGLRILQRFVVDICKASTDWTPEHIIDEAIN
                     KIREQVGTEKVLLGLSGGVDSSVVAALLHRAIGEQLVCVFVDTGLLRLNEAEQVLSMF
                     GRHMGIRIIAVNAEDKFLTALKGVTCPEEKRKIIGRTFIEVFDEEAQKLADIKWLAQG
                     TIYPDVIESAATSTNDAAVVIKSHHNVGGLPDTLNLKLLEPIRELFKDEVRQVGLELG
                     LPHDMVYRHPFPGPGLGVRILAEVKKEYADILRKADAIFIEELHNAQLYHKISQAFAV
                     FLPVKSVGVMGDGRRYDYVICLRAVETVDFMTAHWSQLPWNFLGKVSNRIINEVEGVS
                     RVTYDISGKPPATIEWE"
     gene            complement(6931..8403)
                     /gene="guaB"
                     /locus_tag="AC06IO_001712"
     CDS             complement(6931..8403)
                     /gene="guaB"
                     /locus_tag="AC06IO_001712"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIO9874891.1"
                     /translation="MPLSIVQQSLTFDDVLLVPAHSTILPKDVSLKTKLTRAIHLNMP
                     LISAAMDTVTEARLAIALAQEGGLGIIHKNMSIADQAEEVRRVKKFESGMVKDPISVT
                     PDLTVKELLAVMTKYNFSGVPVVDGKHLVGIVTSRDIRFETNMNLTVAQVMTPKGRLV
                     TVKEGASREEVRSLLHKHRIEKLLVVNESFELRGLITVKDIQKAKDNPYACKDSFEQL
                     RVGAAVGVGEGTDERVAALVESGVDVIVVDTAHGHSQGVLNRVKWIKKNYPDVQVIGG
                     NIATAAAARDLYEAGADAVKVGIGPGSICTTRIVTGVGIPQISAIANVAQELKGIIPV
                     IADGGIRFSGDVCKALAAGADTVMLGSMFAGTEESPGEIELYQGRTYKNYRGMGSIGA
                     MSLAQGSSDRYFQDASLGTEKLVPEGIEGRVPYKGPVQTIIHQLLGGLRSCMGYTGCA
                     TIEELHSKTEFVQVTNAGMRESHVHDVSITKQAPNYQVDN"
     gene            8612..9442
                     /gene="minD"
                     /locus_tag="AC06IO_001713"
     CDS             8612..9442
                     /gene="minD"
                     /locus_tag="AC06IO_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIO9874892.1"
                     /translation="MAKIIVITSGKGGVGKTTSSAAISSGLALLGHKTVVIDFDIGLR
                     NLDIIMGCERRVVYDFINVINGEANLNQALIKDKRIPNLCILPASQTRDKDALTLEGV
                     EKTLNELAKDFDFIICDSPAGIETGALMAMYFADHAIVVTNPEVSSVRDSDRILGILA
                     SKTKRAIENKAPVQEHLLLTRYDPERVERGEMLSVTDVKEILAIPLIGVIPESKSVLK
                     ASNTGTPVILDESSDAGIAYQDAIARFLGEERPMRFISIDKKGLFRRLFSKNKEEVTI
                     "
     gene            9439..9708
                     /gene="minE"
                     /locus_tag="AC06IO_001714"
     CDS             9439..9708
                     /gene="minE"
                     /locus_tag="AC06IO_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIO9874893.1"
                     /translation="MSIFNYLRRRASTASVAKERLQIIISHERSQRNTPDYLPKLQEE
                     ILAVIAKYVNISRDQVSVNLERMEDSAVLELNITMPDKALEDSNS"
     gene            complement(10023..12509)
                     /locus_tag="AC06IO_001715"
     CDS             complement(10023..12509)
                     /locus_tag="AC06IO_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIO9874894.1"
                     /translation="MSELILILAAIGVAGTLLTRHASISVWAISYTIFLGLVLQFSSP
                     GLLSQIVLWAIEAVLVLGSIKPLRREVLSKHLFQIIKKTMPSMSITEREALEAGTVSW
                     EGDLFSGNPNFSLLRNAPTVALTEEEQAFIDGPVSALCEMLDDWDITHNRTDLPPEVW
                     QFIKENRFLGMIIPKQYGGLGFSATAQMSILVKIYGRSITAATTISVPNSLGPGELLL
                     KYGTEEQKNYYLPRLADGREIPCFALTGPNAGSDAASIPDKGVVCRQEFNGELILGVR
                     LNWDKRYITLCPVATVIGLAFRMFDPDNLLGKGEDIGITCALIPATTPGITKGRRHFP
                     LNTGFMNGPTQGKDVFIPLDYLIGGASMAGQGWRMLMECLSAGRAISLPSSASGGAQA
                     VALASGAYARIRKQFNQPIGKFEGIEEPLARIASAAYIIDSSLTMAAAAIDNGAKPSV
                     AGAILKYHTTERARQLALDGMDIHGGKGICLGPNNYIGRGYQGSPIGITVEGANILTR
                     SLIIFGQGAIRCHPYVFKELESIRNNDLVAFDDSFFAHVGFVITNFTKSLIFGWTDAR
                     FSKSPASSVKRYYQLIHKYSSNLAFLADFSMIVLGGELKRKEKLSARLGDMLSCLYMA
                     SAVLKRFYEDGEPRADLPVVHWSCQQLLHECETAMHGVIINFPARWARIALSLILKPH
                     GNRRHKPDDKLGHKVARILIEPNETRNRITRLVYKKPGDNCPLGRMEEAFHKICAVEE
                     LERKVMKAVKDNILHSLTFQEQILEALEHQILTPVEAKQLEEAEKARQDVIRVDDFAD
                     EELRRPSSLGEKVKSEKKIPARDDMETETA"
     gene            complement(12602..13387)
                     /locus_tag="AC06IO_001716"
     CDS             complement(12602..13387)
                     /locus_tag="AC06IO_001716"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9874895.1"
                     /translation="MRELIHFAHGNGFPSLCYKQLLEQLEARFDCCFIDKIGHDPDYP
                     VGENWHNLVTEIIASIKRQAEQPVIAVGHSLGGVLSLLAAIEQPKLFKAVIMLDSPLI
                     GAFKSSMVRLAKALGIIDRVTPAFRTKKRREHWQNYEQLISYLKTRDLFKTFTEECLN
                     DYIKYGLQYKEDGYYLRFDRHIEYQIYRTIPHVIPRYKGKLIVPAALIYGDKSTVVDR
                     MDIRYMKNHFNVKCFKTMGTHLFPMEHPKAVGKQVIEVIDAII"
     gene            complement(13384..14301)
                     /locus_tag="AC06IO_001717"
     CDS             complement(13384..14301)
                     /locus_tag="AC06IO_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HIO9874896.1"
                     /translation="MYLMREQENVWESGVAKEKNKLKPIDAPIYSYWQALYMSFYSKR
                     LFVDVGKHWRGIGLIYLLLVIAICSIPFSIRIASDFNKTFNQQIIEPLLQLPTVYVQK
                     GNVIFDKPMPYLIRNDKNQVVIIIDTTGKVVDFTEEYPYLNILIKKNSINFLLPTPEL
                     LGSSPVNEQKGVPIVQPLDKDMNMVFDGKKIIQDNAINGLKYASQLMIYPIVVAILYS
                     IMVTVLLVIAFLGQVFSKIFFSFTISFKKSSRLLMVASTPMLVVLVTMLSLHFIFPGF
                     GVILLILLAAYYSYGVYGLRDESRKMVLK"
     gene            complement(14252..15343)
                     /locus_tag="AC06IO_001718"
     CDS             complement(14252..15343)
                     /locus_tag="AC06IO_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9874897.1"
                     /translation="MATLNLIEVSKQVGETTILNRINVDIKKGEFMVVVGPSGCGKST
                     LLRLIAGLDVLSSGKILINNQCVNDTEAAKRDMAMVFQNYALYPHMTVFDNMAYGLKL
                     RRFSKADINQRVTEVANLLHLTPYLDRKPQALSGGQKQRVAMGRAIVRSPAVYLFDEP
                     LSNLDAKLRTEMRHEIKRLHQQFNTTSLYVTHDQTEAMTMADRILVLNQGEVEQIGTP
                     QELYQSPATQFVAGFTGHYPINFISGKYDKKTDSIYTEIGIVYSAPNWLHSVDDNADL
                     IVAIRPEHIQLCSSQSQDAVELHVEFVDDMGADKLIQAKCVKNNEQINLRISAEQMVS
                     NRQLHVELPKLKLHVFDERTGKRVGEWRG"
     gene            complement(15343..16170)
                     /gene="ugpE"
                     /locus_tag="AC06IO_001719"
     CDS             complement(15343..16170)
                     /gene="ugpE"
                     /locus_tag="AC06IO_001719"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HIO9874898.1"
                     /translation="MRQFKRFGSHGLILFFIGLMMAPLYLAVVAASHEGTVMMHSQLP
                     LIPGTSFFKNIKSVLTEGLAVTGGEPITSMLVNSLFMAFIIAVGKIILALGSAFALVY
                     FDFPFKKSCFALIFATMMLPVEVRIVPTFQVVASFGLLNSFSGLTLPLLASATGTFLF
                     RQFFKTISKELVDAAKLDGAGPLRFFIDIVLPLSRIQISALFVILFVYGWNQYLWPLV
                     ITTETKMTTIVMGIRYLAGVADQVPEWHYIMAVALIALVPPCMVVMVMQRWFEKGLK"
     gene            complement(16167..17045)
                     /locus_tag="AC06IO_001720"
     CDS             complement(16167..17045)
                     /locus_tag="AC06IO_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIO9874899.1"
                     /translation="MSKFNHQRLFAWLFIVPQLIVTIIFFIWPACSALLQSFYYTDAF
                     GLHKNFAGLTNFFDLFLDPGYAKAIWVTFVIAISVTLITMSLGLLMATLINYRGKSQG
                     VYKTLLIWPYAIAPAVAAILWRFLCHPTLGWLTQVLEFLGIHFNYVNDANQALLVVIL
                     TASWQQFSYNFLFYFAALKAVPQTLVDAAIMDGANAWQRFWQIIFPILSPTTFFLLIM
                     NLIYGFFDTFGIIQVMTHGGPGNSTTNLIYKVYEDGFEGMDLGSSSAQSVLLMIIVIA
                     VSLLQFKYLEKKVHYE"
BASE COUNT         5469 a         3587 c         2913 g         5137 t
ORIGIN      
        1 agaacaagag ctttatcaaa taatttattg ttataacaat gcatgacgat atcgaaatca
       61 ttctcatcga tgtatgtaaa gtattcattg atatcaatag gattttgtcc tattaaaaat
      121 tttacaacag attgaaaatc aaggttattc agtactaatg cggcattatg taaaaaagtc
      181 ttgtctgatc ggtcatgcac taaaagatta acctgatttt taatcaatag ctgaatgata
      241 tcaatgtatt tttgggtatc cttcgctaaa gaatatcctt tcaatgtcaa actcaaagga
      301 caatgaccag aatgactttg ctgttgatcg atattagcac catgttcaat aagccatttt
      361 acattttcaa cttgtccgag ataacaggcg accatcaaag gggtgaatgt atccgcttca
      421 tcaaaatcta atattttttc gtcagagccc acttttaaaa atggcaaatc gatatctgag
      481 acatttaatt cttgttcttt tgcaagagaa tagttggcaa aaattatccg tcctatttga
      541 gtcattaaaa ctctatcccc tgctttaaga tcaaatcctt ccaaattata gggcatttgt
      601 tctaatcggt attgagattg gtgagtttga taaaattcct gtgcagatag ccaaccatct
      661 ccaggattaa gaaagtaata atacctggag gcttgctctt cgtaagtatt atagttgatt
      721 aatagttgtt gccctgcctt gatgtttttg gtggttatta cttttacaac tttcttgcga
      781 ttcaatgtac cttcaacaaa ttctgcgtta tcctgactat cagaaaaatt gatataacga
      841 gtcaaattac cttttcttgc cgcatctact acccggcccc cgacatacat cgcataactt
      901 ttatcagaac caacatgttc cattagatat tgttcaaatt cttgttcaga atacacttct
      961 cctgtgtaaa taccaatgca cgttcctttt ggaatgtctt ctctggcaaa taaacctcgt
     1021 ccaccggttt ttctgagtaa atggacaggt tgaacaataa cgagatcggt tctgtctata
     1081 gcagacggaa tgtaatcatt gccagacatg ttatcaatat gtaaatcaac cataatcttg
     1141 ctcgtggcaa tcaattcatg attactttcc ggtccattga caggagtaaa cagcgtgtaa
     1201 atattacctg ggaggtgtgt catcccaggt ccttttttct ttgcctttct ttgggaaaat
     1261 ttaaatttag atttgtgctc tttactacca tgattcaatt gctgttcttt aaactttgac
     1321 tgaagggcac ttttcttttt aagtttgcta tcattcttgc ttcttggcat aacactgctc
     1381 tcttgtaaaa aatcatataa aataatcatt aaggagtcat tatactctat tagagtattt
     1441 tttgctctat tttgcatgtg attttcattg aaaatctatt attttgctga ttgaagagta
     1501 ttgttgctcc tcttacccat gtgctaacat ttatgatttt ggataaacca taatgggcgt
     1561 tacaattaaa actcctgatg aaattgaaaa aatgcgtgtc gctggcagac tggcagccga
     1621 agtacttgag atgattggtc cttatgttca agttggcatt acaactgatg aattaaacac
     1681 tatttgccac gattatattg ttaatgtaca aaaggctatt cccgcccctt taaattataa
     1741 cggtttcccc aaatcaattt gcacctcaat taatcatgtg gtatgtcatg gaataccagg
     1801 gaaaaaaact ttaaaagatg gagacattat caatattgat gtcactgtta taaaaaatga
     1861 ataccatggt gataccagta aaatgtttat tgtaggcact ccttctgtca aggccaagca
     1921 tgtagtgcag atagcacatg agtgcttgta tattggaatc gatatggtta aacctggtgt
     1981 ccagctaggc gatatagggc atgccattca gcaacatgca gaaaaaaatc gttgctctgt
     2041 ggtgcgcgat tactgcggac atggaattgg ccgtgtattt catgaggatc cacaagtgct
     2101 tcactatgga gttcccggaa cagggatgag gttagaacca ggtatgactt ttaccataga
     2161 acctatggtc aacataggaa aacaccatac tcgattattg ccggatcatt ggactgtcgt
     2221 taccaaagat catagccttt cagctcaatg ggagcacacg cttttggtaa ctgatacagg
     2281 tgttgaaatt ctgactttgc gtaatgaaga acgataatcg catcataaaa aatacgatta
     2341 aacagttcaa agagaagctg tgcagggaca ttagccaaaa agctaatatt acctccatca
     2401 cacgcaagct tgctgtattt attgatacta ttttaattca attatttatt aaaaataagc
     2461 tacactttgg agataatttt tgtctcctgg ctcttggaag ctacgggcgt agagagcttc
     2521 aacttcactc agatattgac ttgttaatat tgcacactga aaaagtaagc aatatccaat
     2581 tacaacgcgc acaaaaattt atccaggatt gttgggatgt ggggctggag gtaagccatc
     2641 aaattaccac tgtttcttca tgtgccaatt tggccagtca ggatttgagc gtaatttcta
     2701 ccattatgga tatgttctta ctgtgtgggc atggtgcctt aatggaggaa ttaatatacc
     2761 aaacacacac cttgcacatg tggcctagcc atcaatactt ctttgccaaa cttcaggaac
     2821 agcaaagtcg ttatgcaaag tatggagaaa cagcctacaa ccttgaacct aatattaaaa
     2881 atggtccagg tggattaaga gacctgcaaa ttttgctaag cattagcaaa cgccatttta
     2941 aaattaaaaa attagccgag ggaatcggct acggctttat tactgataaa gagtatgaag
     3001 aattaaaata ctgccagaac tttctttggc gcgttcgatt tgccttgcat atgttagcag
     3061 gaaagcctga ggagcgcttg tcatttgatt atcaggttaa attagctcaa ttttttggat
     3121 atcaagatca gtctcatatc ttggccatag agcaatttat gaaagactac tttaaagtca
     3181 tcaaacgcaa tagagagctg aatgaaatgc tcttgcaatg gttcaatgaa accatagtct
     3241 atcatcaaaa gcaaaaaatt attcgtctgg atgatgaatt tcaactgtca aaccgattca
     3301 ttgaagtacg caataaccgt gtatttaaac aaaatccgca atctatattg aaactattct
     3361 attggcttgt taaacgccct gacatagaag gagtaagggc aagcacaata agattaatta
     3421 gagaatcatt atttttaatg ggcaaacgct ttagagaatc aaaagaaacc gctaatatat
     3481 ttgtcaacat attcagaact ggtaatgacc cctacgatgc attacaaaga atgaatagat
     3541 atggtgttct agcgcactat cttgattgtt ttgctacagt cactggacaa atgcagtatg
     3601 atttattcca tgcctatact gttgatcagc atacactatt tgtgatccgt aatatatctc
     3661 gctttaaaaa gaacgaatat gctaaacaat ttcctttatg cgccaaaatc attactgctt
     3721 tagagaagcc cgaaattttg tatctcggcg ctttattcca tgacattgcc aaaggtcggg
     3781 gaggagatca ttctgaatta ggagcaattg aggcacaaca atttacacaa cgccactata
     3841 tggagacaga agatagcaaa ttgattgttt ggcttgttcg ctaccattta ctcatgtcac
     3901 aaactgctca acgtaaagat atttatgatc ctaaaaccat tgagcaattc tgccaacttc
     3961 tacctcatgc aaagtatttg gattatcttt atttactcac agtagcagat atttgcggta
     4021 caaatcctac tctttggaat gcctggaaag attcattact taaagaatta tatcatgcag
     4081 caaaaacaag acttcataaa caacaagagt tgctcgatga agcagctctg atatctattc
     4141 gtaaacaata cgctatggat atattaattt cagatgggat atcctccaga gtcatacagg
     4201 atttatggaa ccaatttaag ggaaaatatt ttcttcatga atctcctgag gtgatagcca
     4261 ggcacaccaa ggccatactg aacagcaaac aatttccagt ggtaataatt atgccgcatc
     4321 acagtcaggg tggaaccgaa gtatttatct acatgccaca taaagatgag agatttacta
     4381 tcaccacttc agtattaagc aatcatcacg ttactataca ggaagcagca attattactt
     4441 gtgataatca atttgatctg gatacttata ttattttaga tgaaaacaat caggcttttt
     4501 tgaatgaaca acgtgccaga gacatccaaa aaagtctatg tgatcatctg gccaatacag
     4561 gacgcctccc ggctgtttct cgcagaagat tatccagagc attgactcat tttaatgtca
     4621 aaacacaaat taactttatt gatgacaata cgaatcatca aacccaacta tttctcgtta
     4681 ccaatgacag gcctggactt ttggcgacta taagtcgtgt gtttcttaca ctaaacattc
     4741 atttacataa tgcaaaaatt gctacggctg gtgagcgggt agaagatatg ttttacatct
     4801 ccaaccagac aggttattcc ctgaatcatg aggaaaaaac aattttaaaa gaaaaattaa
     4861 ttttggaaat ttctaaaagc aagtattaag aaatatgcca agataaatta ctagcgaagc
     4921 tttctgaaaa aatctgacag caattgagaa cactcagcct gcataatgcc ttcatctatt
     4981 aataccgaat ggtttagagc atgtaaaaca ttaaacattg agccagctgc ccctgcttta
     5041 aaatctcggg tagcaaatac cagccgtttg atgcgtgcgt gaaccataag tcccgcacac
     5101 atcacacagg gttcgagagt aacataaagc gttgtatcca ataatctatg attatttagc
     5161 tttctcgagg cttgtcgtat tgccctaatt tcagcatgat cagaaggatc atgacttttt
     5221 tcaatgacat ttctgccaac gcctaataat tgattatctt tgctaaccaa cacagcccca
     5281 acaggaacct ctccttcatt acgagctata accgcttgct cgtaagccag ttgcatccaa
     5341 aatttatcaa tcattcccat tcaatggtag caggtggctt gccagaaata tcgtaagtga
     5401 cacgtgatac tccctctact tcattaataa ttcgattgga tacttttcct aaaaaattcc
     5461 agggcaattg agaccaatgt gctgtcataa aatcaactgt ctcaacagct cttaaacaaa
     5521 ttacgtaatc atatcggcgc ccatccccca tcacacccac gctctttact ggtaaaaaaa
     5581 cagcaaatgc ctggctgatt ttatgataaa gctgcgcgtt atgcagttct tcaataaaaa
     5641 tagcatcggc tttacgtaat atgtccgcat attctttttt tacttccgct aaaattctta
     5701 cacccaatcc aggtccagga aagggatgtc gataaaccat atcatgcggg agtcccaatt
     5761 ccagacctac ttgacgtact tcgtctttaa agagctcacg aataggctcc aacagtttaa
     5821 gatttaatgt atctggtaag ccaccgacat tatgatgaga ctttatgacc acagccgcat
     5881 cattagtact tgttgctgcg gactcgatga catcggggta gattgtgccc tgagccagcc
     5941 actttatatc agccagcttc tgtgcttcct catcaaagac ttcgataaat gtacgcccaa
     6001 taatttttct cttttcctct gggcaagtta ctcccttcaa agcagttaaa aatttatctt
     6061 cggcattgac tgcaataatg cgtattccca tatgccgacc gaacatgctc agcacttgct
     6121 ccgcctcatt aagtctgagt aaacccgtat caacgaaaac acaaaccagt tgctctccaa
     6181 tggctcgatg aagtaatgcg gctacaacag aagagtcaac acctccggat aggcccagta
     6241 aaactttttc agttcctacc tgttctctga ttttattaat ggcttcatca ataatatgct
     6301 ctggagtcca gtctgttgat gctttacaaa tatctacgac aaaacgttgt aaaattctta
     6361 aaccttgcaa ggtatgagta acttctggat gaaattgcaa accatacatt tgacgacttt
     6421 catcagccat acctgcaatt ggtgcattac gtgtttcaca tatcacttta aaacctggtg
     6481 gtaactcagt gaccttatca ccatggctca tccaaacatc cagcagagcg ctgccatcta
     6541 tcgcggtacg atcttcgata ttacttaata attgactatg gccatgcaaa cgcaactcag
     6601 catatccaaa ttctcttagc gtcgatgaat gcacttgacc accgagttgt acagccatgg
     6661 tttgcatccc atagcatatg cccagcaagg gcaaatcgct ctcaaacagc cattgaggag
     6721 ccctgggatt ggcatcatgc gttactgtcg aaggacctcc tgataaaatc acaccacaag
     6781 ggtttaattt tttaaattgt tcatgattaa tattataagg ataaatttca caataaaccc
     6841 ccatttctcg aacgcgtctt gcaattaatt gggtatattg agaaccgaaa tctaatatta
     6901 ataatgggct ttttttcaaa tcattcatta ttaattatcc acctgataat tgggggcttg
     6961 cttagtaata ctcacatcat gcacatgtga ctcacgcata ccagcattgg tcacttgcac
     7021 aaattcagtt ttagaatgca attcctcaat agtagcacaa ccggtatagc ccatacagga
     7081 gcgtaaacca cctaataatt gatgaataat tgtttgaacc ggtcctttat aaggtactct
     7141 tccctcaata ccttcaggaa ccagcttttc agtaccaaga gaagcatcct gaaaatagcg
     7201 gtcacttgac ccctgagcta atgacatggc tccaatggat cccatacctc tgtaattttt
     7261 ataagtacgc ccctgatata attcaatttc accaggagat tcttctgtac ctgcaaacat
     7321 actaccaagc attacagtat cagcacctgc ggccaacgct ttacaaacat cacctgaaaa
     7381 acgtatccca ccatcagcta tcacaggaat tatcccttta agttcttgtg ccacatttgc
     7441 tatagcacta atttgcggga taccaacacc ggtcactatg cgagtggtac aaattgatcc
     7501 tggcccaatt ccaactttta ctgcatcagc acccgcctca tataaatctc ttgcggctgc
     7561 agcggttgca atattaccac ctatgacttg tacatcagga taattctttt taatccattt
     7621 cacgcgattt aatacgccct gagaatgacc atgcgctgta tccactacta tgacatcaac
     7681 tccggattca accagagctg caactcgctc atcggtccct tcaccaacac caacagcagc
     7741 cccaactctt agctgctcaa aactgtcttt acaagcatag gggttgtctt ttgctttttg
     7801 tatgtctttt actgtaatga gtccacgtaa ctcaaaactc tcattcacta ccaataattt
     7861 ttctattcgg tgcttatgga gcaaacttct tacttcttct ctactcgctc cttcttttac
     7921 agtaaccaaa cgccctttgg gagtcattac ttgcgccaca gtcaaattca tattggtttc
     7981 aaaacgaata tctcgactgg ttactattcc tactaaatgc ttaccatcaa ccaccggcac
     8041 ccctgaaaaa ttatattttg tcatgactgc aagcaattct ttaactgtca agtcgggagt
     8101 tactgaaata ggatctttca ccataccgct ttcaaatttt tttactcttc tgacttcctc
     8161 tgcttgatca gcgatactca tattcttatg aataatcccc aaaccacctt cctgtgctaa
     8221 agcgatagct aacctcgctt cggtcactgt atccattgcc gccgaaatta aaggcatatt
     8281 aaggtggatt gcacgggtta atttggtttt caatgaaacg tctttgggta aaatagttga
     8341 gtgggcaggg acaagcaaaa catcgtcaaa ggttaatgac tgttgcacaa tggatagagg
     8401 catttttatc tcactccagt aattaaataa ccaaatagtt tactacaaac tttaaaaaat
     8461 ttaaacattt attgtataaa tatatcacta caacctttta attagttttt ataacaatta
     8521 agctttggat aacttgaatt aatgaatgaa taacgtgtaa actgttggtt aaatattgtg
     8581 ttgaatcgaa aaaaatagga agggttcagc gttggctaaa ataatagtaa ttacatctgg
     8641 gaaaggcgga gtaggtaaaa ctacttcatc agcagcgatt tcttcgggac ttgcactctt
     8701 agggcataaa actgttgtta ttgattttga tatcggttta agaaacctgg atatcattat
     8761 gggctgtgaa agacgtgtag tttatgactt catcaacgtc atcaatggtg aagccaactt
     8821 aaaccaagct ctcatcaaag acaaacggat ccctaacctt tgcatactgc ccgcgtcaca
     8881 aactcgtgat aaagacgcac taactctgga aggagtagaa aaaactctca atgagctagc
     8941 aaaagatttt gatttcatta tttgtgactc ccctgctgga attgaaactg gagcacttat
     9001 ggcaatgtat tttgctgatc atgccattgt tgttactaat cctgaagtct cttctgttag
     9061 agattcagat agaattctgg gaatactggc cagcaaaaca aaaagagcta tagaaaataa
     9121 agcacccgtt caagaacact tgttattgac acgatatgat cctgagcgag tagaacgagg
     9181 tgaaatgtta tctgtaactg atgtaaagga aatattagct attcctttaa ttggtgttat
     9241 acctgagtcc aaatcggttc ttaaagcatc caatactggt acgccagtta ttctggatga
     9301 atcaagcgat gcgggcattg cttatcaaga cgccatagca cgctttttgg gcgaagaaag
     9361 accaatgcga tttataagta ttgataaaaa aggactattt cgtcgcttat tcagtaaaaa
     9421 caaggaggaa gtgacaatat gagtatcttc aattacttac gcagaagagc ctccaccgct
     9481 tctgttgcta aagaacgctt acaaatcatc atttctcatg agcgttctca gcgcaataca
     9541 ccagactatt tgcctaagct gcaagaagaa attcttgcgg ttatagcaaa atacgtgaat
     9601 ataagccgag atcaagtgag tgttaatctg gaacgcatgg aagatagtgc cgttcttgag
     9661 ctaaacatta caatgccaga taaagcattg gaagacagca actcataatt ctattaagtt
     9721 ttttatactt ataggtatta ttacactatc aaataatgta gtttacctgc ctgtttaaca
     9781 tacgcattaa aacattatct ccttcaagaa tatttcaaaa aactttaaat aaaataccta
     9841 gatgagttac gcattacatg catgggagtt aaactcattc ttaatggctc tgtctgtaaa
     9901 gatcaattga ttaaaaccaa aattgataaa aacatatggg aatgattagc ctgactctaa
     9961 ttgctgaata ggattacatc atgtaactct attcagcata atagaaaaga atctagtata
    10021 aattaagctg tttcagtttc catgtcatcc ctggctggaa tctttttttc tgactttacc
    10081 ttctcaccaa gtgacgaggg gcgacgtagt tcttcatcag caaaatcgtc tacccttata
    10141 acatcctgtc tggccttctc agcctcttct agttgtttcg cttcaactgg agtcaatatt
    10201 tgatgctcaa gcgcttccaa tatttgctcc tggaaagtca atgaatgaag gatattgtct
    10261 ttaacagcct tcatcacttt gcgctctagc tcttcaactg cacaaatctt atgaaatgct
    10321 tcctccatcc ttcctaaggg acaattatcc cctggcttct tataaaccaa tctcgtgatt
    10381 cgatttcttg tttcattagg ctctatcagt attcttgcta ccttatgacc caatttatca
    10441 tccggtttat gccttctgtt gccatgaggt tttagaatta acgataaagc aattcttgcc
    10501 cagcgtgccg gaaaattaat gattacccca tgcatagctg tttcacactc atgcaataac
    10561 tgctgacaac tccaatgaac aacaggtaaa tcagcacgag gttccccgtc ttcgtaaaat
    10621 cgttttaaaa cagcggatgc catgtataaa caactcagca tgtcacctaa gcgtgccgat
    10681 aatttttctt ttcgctttaa ctcaccacct aatacaatca ttgaaaaatc tgctaaaaaa
    10741 gccagattgg aactgtactt atgaattaat tgataatatc tttttacaga actggctgga
    10801 gacttgctaa aacgagcatc ggtccatcca aaaataagtg actttgtaaa attagtaata
    10861 acaaaaccca catgggcaaa aaaggaatca tcaaacgcaa ctaaatcatt atttctaata
    10921 ctttctaact ccttgaaaac ataaggatga caacgtatcg ctccttgccc gaatataatt
    10981 aagcttctgg ttaaaatatt ggccccttcc accgtaatgc caataggaga accttgatat
    11041 cctcttccaa tataattatt tggaccaagg catatccctt taccaccatg tatatccatc
    11101 ccatccagtg caagttgtct cgctctttca gtagtatgat actttaaaat agctcctgcg
    11161 acagaaggtt ttgctccatt atctatagcc gctgctgcca tagttaaaga agaatcaatg
    11221 atataagcag ctgaggctat tctggcaaga ggctcttcta tcccctcgaa cttgccgatt
    11281 ggttgattga attgtttacg gattctggca taggcgccag atgctaatgc aacagcttgt
    11341 gcacctccac tggcgcttga tggcaaagag attgcacgcc ctgcgcttaa acactccatt
    11401 aacattcgcc acccttgacc agccatagat gcaccaccga tcaaataatc aagcggaata
    11461 aagacatctt tgccttgagt tggaccattc ataaaccctg tatttaaagg gaagtgcctg
    11521 cgcccttttg tgatccccgg agtagttgca ggaataagcg cacaagtaat accaatgtct
    11581 tcacctttgc ctagcaagtt gtccggatca aacattctaa aagccaaacc aataactgtc
    11641 gccaccgggc acaaggtaat atatcgtttg tcccaattca aacgaactcc caatatgagc
    11701 tcaccattga actcctgccg gcaaacaaca cctttatcag gtatagaggc cgcatccgaa
    11761 ccagcattag gtcctgtcaa ggcaaagcat gggatctccc tcccatcggc taatcttggt
    11821 aaataataat ttttttgttc ttctgtccca tactttaaaa gtaattcacc aggccccaat
    11881 gaattaggta ctgaaatagt tgtagcagca gtaatagaac ggccatagat ttttaccaaa
    11941 attgacatct gagcagtagc ggaaaaacct agtccaccat actgctttgg aattatcatt
    12001 cccagaaaac gattctcttt tataaattgc caaacttcgg ggggaagatc tgttctatta
    12061 tgagtgatat cccaatcatc taacatttca cagagggcac tgacagggcc gtctataaaa
    12121 gcctgctctt cttcagttaa tgcaacagtt ggagcattcc tcaaaaggga gaaattagga
    12181 ttaccactaa ataaatctcc ttcccaactc actgtacctg cttccaatgc ctctctctca
    12241 gtaatagaca tggagggcat cgttttttta attatttgaa acaaatgttt tgatagtact
    12301 tctcttctta aaggcttaat ggagcctaaa accagtacag cttcgattgc ccataagacg
    12361 atttgcgaca acaggccagg agagctgaat tgcaaaacca agccaagaaa aatggtataa
    12421 ctaatagccc atacagatat cgatgcatgt cgggttaata acgttccagc cacaccaatt
    12481 gctgctagta ttaatataag ttctgacaca acattctcct tattataggc taatccaaat
    12541 gaaagaattt aatagacttc tttctcgata acaaaatctg caaaccagtt aagacgtcta
    12601 atcaaattat agcatcaata acttctataa cttgctttcc aacagcttta gggtgctcca
    12661 ttggaaataa atgtgtaccc atagttttaa aacattttac attaaaatga tttttcatat
    12721 atcgaatgtc cattctatca actacagtac tcttgtcacc atagatcaat gcggcgggaa
    12781 ctataagttt acctttatag cgaggaataa catgcggaat tgttctatat atttgatatt
    12841 caatatgcct gtcaaaacgc agatagtatc cgtcctcttt atattgcaaa ccatatttaa
    12901 tatagtcatt caagcattct tctgtaaatg ttttaaataa atccctggtt tttaaatagg
    12961 aaattaattg ttcatagttt tgccaatgct cacgcctttt ttttgttcta aaagcagggg
    13021 ttactctgtc tatgatacct aaagccttag ccaatctcac catgcttgat ttaaaagcac
    13081 caatgagagg tgagtctagc ataatgaccg ctttaaataa tttcggttgc tctatcgcag
    13141 cgagcaaact caaaacccct cccaaagaat gtccaactgc aatcactggc tgctctgctt
    13201 gtcgtttaat gcttgcaatg atctcagtaa ccaggttatg ccagttttct ccgacaggat
    13261 aatcaggatc atgtccaatt ttatctataa aacaacagtc aaaacgagcc tccagctgct
    13321 ctagtaactg tttgtaacat aatgatggaa atccattgcc atgagcaaaa tggattaact
    13381 ctctcatttg agcaccattt tcctgctttc atctcggaga ccatagacac cgtaactgta
    13441 ataggctgcc aataatatca acaaaatcac tccaaatcct ggaaaaataa aatgaagtga
    13501 taacatggtt accaaaacta ctaacattgg cgttgatgcg accattaaca gacggctgga
    13561 ttttttaaag gatatagtga acgaaaaaaa gattttggaa aatacttgtc ctaaaaacgc
    13621 aatgaccaat aaaacagtca ccataatcga atataaaata gcaaccacta ttggatatat
    13681 catcaattga gaggcatatt tcaaaccatt aatcgcatta tcctgaataa tttttttgcc
    13741 atcaaatacc atattcatgt ctttatctaa tggctgaaca ataggaactc ctttctgttc
    13801 atttactggg ctgctaccca ataattctgg tgttggcaat aagaaattaa tactattttt
    13861 cttaatcaga atattaaggt aaggatactc ctctgtgaaa tctaccactt tgcctgttgt
    13921 gtctataatt attaccactt gatttttatc atttctgatt aaatagggca ttggtttatc
    13981 aaaaataaca ttaccttttt gtacataaac agttggcagc tgcagcaaag gttcaatgat
    14041 ttgctgatta aacgttttat taaaatcaga agcaattctt atagaaaacg gtattgaaca
    14101 aatagcaata accaataata gataaatcaa accaattcca cgccaatgtt ttcctacatc
    14161 aacaaaaagc ctctttgaat aaaaagacat atacagcgct tgccaataac tatagatagg
    14221 agcatcaatc ggttttaatt tatttttttc tttagccacg ccactctccc acacgttttc
    14281 ctgttctctc atcaaataca tgcaatttca atttgggtag ctctacatgt aattgcctgt
    14341 ttgagaccat ctgctctgct gaaattctca aattaatctg ttcattattt tttacacatt
    14401 tcgcctgaat caatttatca gcccccatat catcaacaaa ttccacatga agttcaactg
    14461 catcttgact ctgactggag catagttgaa tgtgttcggg tcgaattgcc acgataagat
    14521 ctgcattgtc atcgacagaa tgaagccaat tgggtgctga ataaactata cctatttcgg
    14581 tataaatact atccgttttc ttatcgtatt tgccagaaat aaaattaata ggatagtgcc
    14641 cggtaaaacc agctacaaac tgcgtagccg gactttgata cagttcttgt ggcgttccaa
    14701 tttgctcaac ttccccctga ttaagcacca gaattctatc agccatagtc atggcttcgg
    14761 tttgatcatg agtaacgtat aaactggtcg tattaaattg ttgatgcaat cgtttaattt
    14821 catgtcgcat ttcagttctt aattttgcat ccagatttga taatggttca tcaaataaat
    14881 aaactgctgg agaccgcaca atagccctac ccattgctac tctctgcttt tgtccaccag
    14941 acagtgcttg cggctttctg tccagatatg gagtcaaatg caataaatta gctacttcag
    15001 tcactctttg attaatgtct gctttactga agcgcctcaa ctttaaacca taagccatat
    15061 tatcaaagac agtcatatga ggataaaggg cataattttg aaacaccatc gccatatccc
    15121 tttttgcggc ttcggtatca ttaacacatt gattattaat aagtatttta cctgagctta
    15181 aaacatcaag tcctgctatc aatcgtaaca aagtagattt gccacaacct gatgggccaa
    15241 caacaaccat gaattcgccc ttctttatat caacgttaat tcgattcaaa atggttgttt
    15301 ctccaacctg ttttgacacc tcgatcaaat tcagtgttgc cattatttca atcccttttc
    15361 gaaccaacgt tgcatcacca taaccaccat acaaggcggc acaagagcta tcaatgcgac
    15421 ggccataata tagtgccact caggtacttg atctgctaca cctgccaaat accgaattcc
    15481 catcacaata gttgtcattt tagtttctgt tgtgatgacc aaaggccata agtattgatt
    15541 ccacccatag acaaacaaga ttacaaacaa ggcggaaatt tgaattcttg acaagggtaa
    15601 cacgatatca ataaaaaatc ttaatggacc tgcgccatct aatttagcag catcaaccag
    15661 ttctttagat atggttttaa aaaattggcg aaataaaaaa gttccagtag ctgatgccaa
    15721 taatggcaag gttagaccag aaaatgagtt caataggcca aatgaagcaa ccacttgaaa
    15781 agtggggact attcttactt ccactggtaa catcatcgtt gcaaatatca gggcaaaaca
    15841 agacttttta aaaggaaaat caaaatatac caatgcaaaa gcagatccca gagccaaaat
    15901 gatcttaccc actgcaatga taaatgccat gaataagcta tttacaagca tggaagtgat
    15961 aggctcacca ccagtcacag ctaaaccttc tgtcagcaca cttttaatat ttttgaaaaa
    16021 tgaggttcct ggaatcaatg gcaactgaga atgcatcatg accgttcctt catgacttgc
    16081 agcaactaca gccaaataca aaggagccat cattaatcct ataaaaaaca atattaaccc
    16141 atgcgaccca aaacgtttaa attgtctcat tcgtaatgaa cctttttctc aaggtattta
    16201 aattgtaaca aggaaactgc aattacaata atcatcaata atacagattg ggctgatgaa
    16261 cttcccaaat ccattccctc aaaaccgtct tcatacacct tatagattaa attagttgtg
    16321 ctattaccag gacctccgtg tgtcatgact tgaataattc caaatgtatc aaaaaaacca
    16381 taaatcaggt tcattattaa cagaaaaaag gtagtgggcg acaggattgg gaaaattatt
    16441 tgccagaatc gttgccatgc attggcccca tccatgattg ctgcatcaac taatgtctga
    16501 ggaactgctt taagcgcagc aaaataaaat aaaaaattgt aactaaactg ctgccaactg
    16561 gcggttaaaa taacaaccag caaagcctga tttgcatcgt tgacataatt aaaatgaatt
    16621 cccaaaaact caagtacctg agtcaaccat cctaaagttg gatggcataa aaaccgccaa
    16681 agtattgcag caacagcagg ggctattgca taaggccata tcaataaagt cttataaact
    16741 ccttggcttt ttcctctgta atttattaaa gtagccatta aaagacctaa actcatagtg
    16801 ataagcgtca cactaatcgc aattacaaaa gtaacccata tggcttttgc ataaccggga
    16861 tcaaggaata agtcaaaaaa attggtcaat ccggcaaaat tcttatgcaa accaaatgcg
    16921 tcagtataat aaaaggattg caataatgcg ctgcaagcag gccaaataaa aaaaataata
    16981 gtgacaatta gctgagggac tataaatagc caggcaaaca aacgctgatg attaaattta
    17041 gacattaact aaccatgtag ttgttaaaat gataattatc atcgcaatag tttaaaataa
    17101 aattat
//
LOCUS       DBNCWK010000061        16352 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.061, whole genome shotgun sequence.
ACCESSION   DBNCWK010000061 DBNCWK010000000
VERSION     DBNCWK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16352
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.061"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(425..1129)
                     /locus_tag="AC06IO_001721"
     CDS             complement(425..1129)
                     /locus_tag="AC06IO_001721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874900.1"
                     /translation="MGVSLLPLSVVDVFVGDVSVVGGVLSVVDVFVGDVSVVGGVLSV
                     VDVFVGDVSVVGGALSVVDVFVGDVSVVGGVLSVVDVFVGDVSVVGGVLSVVDVFVGD
                     VSVVGGVLSVVDVFVGDVSVVGGVLSVVDVFVGDVSVVGGVLSVVDVFVGDVPVVGGV
                     LPVVDVFVGDVPVVGGVLPVVGDVLVDCGFVELDCCVEELGFGLGSSDSASLFLIPST
                     ALSTRKLAPADILPPT"
     gene            1128..1295
                     /locus_tag="AC06IO_001722"
     CDS             1128..1295
                     /locus_tag="AC06IO_001722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874901.1"
                     /translation="MQMSPPPDSNTNTSPPDLGNTTKYDETSGSSKSGSGSIPDSGSS
                     PDVQPTQFTPT"
     gene            complement(1319..2332)
                     /locus_tag="AC06IO_001723"
     CDS             complement(1319..2332)
                     /locus_tag="AC06IO_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HIO9874902.1"
                     /translation="MTDQAITFDDVLLVPSYNHHESRRVVETTSTDRLGKLTLNLPVI
                     SANMDTITESNMANFMHSKGAMGALHRFMTIEENIQEFKKCKGPVFVSVGCTENELQR
                     AEALRDAGADFFCVDVAHAHAKYVGKTLKSLRQLLGSRCIMAGNVATYAGADYLASCG
                     ADIIKAGIGGGSVCSTRIKTGFGVPMLTCIQDCSRADRSIVADGGIKTSGDIVKALAF
                     GADFVMIGGMLAGSAPTPGEVIQKDDGSKVKRYRGMASREAQEAFLGQMHEWKTAEGV
                     ATEVPFKENPDGIIADIIGGLRSGLTYAGADSISELQRKLNYVIVTQAGRIESLPHKL
                     LEG"
     gene            complement(2355..3761)
                     /locus_tag="AC06IO_001724"
     CDS             complement(2355..3761)
                     /locus_tag="AC06IO_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIO9874903.1"
                     /translation="MDNTNTGLKLFVLDTNILMHDPTAIYHFDEHDIYLPMVVLEELD
                     SHKTGTSELARNVRQTNRMLVELMSNATHDQIVSGLPIPSYINSSNKKCSGRLFFQTD
                     EFDQVVPSTLPGHKVDNTILATALGLQKKYYGKRQVIIISKDINLRIKAGILGIHAED
                     YYNDQVLDDVNLLHRGLHILDNNFWDSHAKDMGSWQESGKTFYKVSGPLVSQWNPNDC
                     LSTEDDQFQALVKKIDGPNAVVQLIRDYTQPKHSVWGITARNREQNFSLNLLLDPDID
                     FVSLQGSAGTGKTLLTIAAGLTQVMDQNRYSEILMTRVTIPVGEDIGFLPGTEEEKMT
                     PWMGALMDNLEVLHSSQVGGSFGRGATQDLLQNKIKIRSLNFMRGRTFLNRYIIIDEA
                     QNLTSKQIKTLVTRAGPGSKIICLGDIKQIDTPYLTETTSGLTFAVDRFKSWEHSAHM
                     TLTRGERSRLAFYAAEHL"
     gene            complement(3916..5115)
                     /locus_tag="AC06IO_001725"
     CDS             complement(3916..5115)
                     /locus_tag="AC06IO_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9874904.1"
                     /translation="MFIRNLQLFIVVASCILVLIGTDILLPSLPNIAHALSVSSNEAK
                     MLISIFMIGQFSTVLIWGVIADRLGRRKTLFLGMLIFFIGSIFSLKADSIQLLLACRF
                     LQGTGAVVVPVAGWALVQDLFPKDEGARIMTWIGTLTAVIPLFAPALGGQLDVLYGWR
                     SNLYCIAFYSFILCVIMLFLPRTIPVQKTTTADLKETLGIYIRIIKNKTFISYIALFG
                     LLNCGEWCFLTVAPFYYSHAHIAADTMGILLMITSMGFVCGSLLASRLFNRLGIDKII
                     ALGIQLAVMSSLMLLIGEYFHWSKYQLYNAITIGVYILSSALLWGATTSRALQCFDDC
                     RGSASAIRSLILLCFAAFGTYSGRLVNHNSLFPVGLFLLFMAFIAFIVFNNKELRTER
                     HMEGIPI"
     gene            complement(5240..5704)
                     /locus_tag="AC06IO_001726"
     CDS             complement(5240..5704)
                     /locus_tag="AC06IO_001726"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIO9874905.1"
                     /translation="MKIGEMVPDFAFKATNNVQGHFNDYKGQIIVLYFYPKDATPGCT
                     TEGQDFRDAYSQFKDLNTVIFGISRDSLKSHEQFKSKQNFPFELISDENETLCQTFDV
                     IKMKSMYGKQVRGIERSTFIIDTQGVLTHEWRKVNVKGHVEEVLNAVRALVG"
     gene            complement(5716..6186)
                     /gene="smpB"
                     /locus_tag="AC06IO_001727"
     CDS             complement(5716..6186)
                     /gene="smpB"
                     /locus_tag="AC06IO_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIO9874906.1"
                     /translation="MTTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAG
                     KINLSDSHVIIKYGEAFLLGAQIQPLPTASTHFIPDPVRTRKLLMNKKELNHLIGSVE
                     RQGYTIVPLSLYWKKNKIKIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT"
     gene            complement(6300..7064)
                     /locus_tag="AC06IO_001728"
     CDS             complement(6300..7064)
                     /locus_tag="AC06IO_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HIO9874907.1"
                     /translation="MKDIIIASFYKFIPLNDFESLREPILTKMHEIGIKGTIILAHEG
                     VNGGFAGNREQMNVFYDYLRSDSRFADLHFKETYDNKNPFDKAKVKLRKEIVTMGVQK
                     VDPSYNAGTYLSPEEWHQFIQDPNVILLDTRNDYEYELGTFKNAINPDIENFREFPDY
                     VQRNLIDKKDKKIAMFCTGGIRCEKTTAYMKELGFQHVYQLHDGILNYLESIPEGESL
                     WEGKCFVFDDRVAVDQKLDRVYPQLPQDYKYEREQK"
     gene            7317..8618
                     /gene="plaC"
                     /locus_tag="AC06IO_001729"
     CDS             7317..8618
                     /gene="plaC"
                     /locus_tag="AC06IO_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase/glycerophospholipid:cholestero
                     l acyltransferase PlaC"
                     /protein_id="HIO9874908.1"
                     /translation="MIQNNRICRWLFIVLLSLPLVGFTSIPVKSIVVFGDSLSDNGNT
                     THLLKSLRQEEDPAFLVAPFKIFVINKMIEFANDYYVPQMVLDAGISAVTDFFDHDLA
                     PYLANLVGKVKQVPVLPGKPYWKNRFSNGRVWIEYLAEMLSIQKSDEEVYLNKAFGGS
                     WSATYDYQLTVWNLIRHPLGTIKNLIVGKLIPPSLGLIVQAYLLEHEQLSDETLYFIY
                     SGSNDYINVLFFEDNYNTEVMSTYIDNVLDGMSSAVLKLANAGARRFVIMGIPHVGDT
                     PKFVKTTDRDVLNAAVDLHNERLAKRIDAWKERYPTADFLFVNTEQYLKKAFEDPEKY
                     GFYNVKDACIDVKFPMFNAFIHSPFARNYVLQYTQVLQYRDKQFAPGENNYHMCSEPE
                     DYLFWDEIHPTTRAHNYLAYEVCMALEEHGYEVTCKMPESA"
     gene            8982..10796
                     /gene="glmS"
                     /locus_tag="AC06IO_001730"
     CDS             8982..10796
                     /gene="glmS"
                     /locus_tag="AC06IO_001730"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIO9874909.1"
                     /translation="MCGIMGAVSERDISKILLEGLRRLEYRGYDSAGIAVIDSQDRLK
                     RVRIQGKVQNLADAMQETAIAGNTGIAHTRWATHGKPSEQNAHPHLSHGEIALVHNGI
                     IENHEHLRQQLITYGYQFTSETDTEVAAHLIHYHYQQHENLLLAVQKSAAEMQGAFAL
                     GVIHQKRPEELVAIRKGSPLVLGFGIGENFIASDALALRSFAQSVIYMEEGDSACVTT
                     QDIKVYDSNRILVQRAAHPLNSDSEIVNKGPYRHFMLKEIFEQSKVITDTLESRINSI
                     DVLRASFGEKASHIFPIVKNIHIVACGTSYHAGMIAKYWLESLAGLPTQVEIASEYRY
                     RDVVVPDNTLFITVSQSGETADTLAALFKAKQSNYLASLAICNVATSTLVREADCVFL
                     TRAGIEIGVASTKAFTTQLAAFLMLAAALCKDNRAQEVLRQLQELPACCERVLQMNEE
                     VESLASLFVNKVHALFLGRGVQYPVALEGALKLKEISYIHAEAYPAGELKHGPLALVD
                     KDMPVIAVAPNDELLDKLKSNLHEVSARGGQLFVFVDDSQNWKANGARLIKVPSCGAW
                     LAPIIYTIPLQLLAYHVAVAKGTDVDQPRNLAKSVTVE"
     gene            10793..11458
                     /locus_tag="AC06IO_001731"
     CDS             10793..11458
                     /locus_tag="AC06IO_001731"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HIO9874910.1"
                     /translation="MNKGQYFWNELWCEGRISFHKKEVNPDLIAYVSSLNIPAKGRVL
                     VPLCGKSVDMLWLVRQGYHVVGIELVEKAILQFVQEHQITVRENTIGQAKQYFTDNLN
                     LWVTDIFALNSALIEPVDAIYDRAALVALPKKLRPAYVDICLKWLKPGGSILLKTLQY
                     NQEKVQGPPYSVSPEEIALSYQQCAKIELLKSQKRIQEPNDHLFNFGISEVNDSVWCI
                     RKG"
     gene            11582..13951
                     /locus_tag="AC06IO_001732"
     CDS             11582..13951
                     /locus_tag="AC06IO_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIO9874911.1"
                     /translation="MAEVMLASAAIIADELKVKVSQVETAIRLLDEGATVPFIARYRK
                     EATGGLDDVQLRFLAERLIYLRELDERRTVILQSIREQEKLTPELEQSILAAETKTRL
                     EDLYLPYRPKRRTKAQIAIEAGLEPLAHALWKDPALDPEEQASQFINSEAGIENTQAA
                     LDGARHILMEHFAEDAELINELREYLWQHGIVKSVGNSTKKDQANKFSDYFDYSEAIK
                     KIPSHRALALFRGRREGILQVSLVLPDQDANYGENKVAAYFNVTDRQRKADGWLIDTV
                     RMTWKIKLFTKLELELLTRLREIADEEAIKVFSKNLRDLLLAAPAGPQVTIGLDPGIR
                     TGVKVVVVDATGKLLDYSVIFPFAPQNEWHQSIADLAKFAAKYQVNLISIGNGTGSRE
                     TERLVADLIKMYPDLKLTKIIVSEAGASVYSASEIAANEFPDLDVSLRGAVSIARRLQ
                     DPLAELVKIEAKSIGVGQYQHDVNQAKLARCLDGVVEDCVNAVGVDINTASAALLSHI
                     SGLNDTLAKNIVQFRDEHGAFTNRNQLKNINRMGEKAFQQAAGFLRIMNGDNALDASC
                     VHPEAYPLVEKIISDKKVSIKEIIGNRELLQSINAEQYVDENFGLPTIRDVLRELEKP
                     GRDPRPEFRTAQFKEGVEDIKDLEQGMVLEGVVSNVTNFGAFVDIGVHQDGLVHISAM
                     TNRFITDPRSVVKAGDIVKVKVVEVDKERRRIGLSMKLNDEQAPAVLQKANKPQPARK
                     QAEQKQPEFKKKQEHKKKPDKIKKSEPVKKTVFNTAMADALAKLKRGGE"
     gene            13954..14334
                     /locus_tag="AC06IO_001733"
     CDS             13954..14334
                     /locus_tag="AC06IO_001733"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIO9874912.1"
                     /translation="MQTPKGELTIQTLAMPLNTNANGDIFGGWIVSQMDLAAGVLAKR
                     ISHGRVATVAINSMTFLKPVHVGDVVSCHVELVKVGNTSMTIRVEVWAYSSIKMEKYQ
                     VTEGIFVFVAIDENGKPRQVPKTQ"
     gene            14331..15971
                     /locus_tag="AC06IO_001734"
     CDS             14331..15971
                     /locus_tag="AC06IO_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2832 family Dot/Icm T4SS effector"
                     /protein_id="HIO9874913.1"
                     /translation="MTNKTQELQRVIADMAVLIEQDAEPDPQLYAFFFQHPEISLELI
                     HLINNLSEELVHDGPPIYSACVFALDICIAQLQAAAEANNKNATKLLSQLMNHLAEVI
                     DTHKHSLSFWLPVLNAFYEVHVELTDDLKEAYYGLASQDDDAVEVDEQFNHLDSIKDL
                     IHELSDLTLFDIAESFFAQSYAMPPDFFADLIVDLYSIEEGKDIALLVLLHPNIEVRE
                     IALSTIDQLMNTITLTPASLSRLQIIKHWYPTHYHGMFDRWIKTQRKKGVTFETEASQ
                     AKITIKATEVDGSGSQGVFIHARKNKKNRLCGLLLKYDLGIKDAWITPEISAAEVTDY
                     YHKAFEDSVTLREVDFSYFQTIAEHFLAITVEKSDIPNLHFLEILEFLGIRLKPDKLD
                     LDCVFEQLSVQIIPFTQEKIAESLKRSKMWLKNKQFTESWYIENPTIDKIVNHNSSFV
                     EGVKVCRLADAMDAVFSEEMELHRDKWQFHFLWIALWVKAKHRKNEKVWQDSFLIAYA
                     IHQGYPLKDIPVMKEICYQTVVNSIETMQERRTHLSQE"
     gene            16159..>16352
                     /locus_tag="AC06IO_001735"
     CDS             16159..>16352
                     /locus_tag="AC06IO_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIO9874914.1"
                     /translation="MDKSTLRQRARNLRQNSTDAERHLWYHLRANRLGFKFRRQVLIG
                     SYIVDFVCIEKRLIIKLDGG"
BASE COUNT         4962 a         3117 c         3470 g         4803 t
ORIGIN      
        1 atatccctga acagtaaact tggaaaagta acctcggaca aaatcctgat gaggtactat
       61 aatttagata tcaacagata tggtaaatta acatggcaga aactccaaaa cccaaatcac
      121 agacaccaac accgaagccg aaacctaaac ctaaggatga gcagactgtt gacccagctc
      181 ctcaacaaag tacgcttggg aaagtaggta gtgtaatcgg aggacttgct aaaaaagggt
      241 taggcaaatt agccgataat atcggcaagc aatttggctc aaatgctcct caacccggtg
      301 ctgaaaataa gcctgcggaa ggacaaaaga aggatgatga caaaaaagat ccgcaaatgg
      361 aaatgattga aaggtatatg aagctggtcg agcaatggca aggcggtatt aacaagcctt
      421 tttattatgt gggtggcaaa atatcagctg gcgccagttt tctggttgat aaggcagtgg
      481 aagggattaa aaataaactg gctgaatcag aggaacccaa accaaatccc aactcctcaa
      541 cgcaacaatc cagctcgaca aatccgcaat cgacaagcac atccccaacg acgggcagta
      601 cgccacctac gacgggtaca tcacctacga atacatcgac gacgggcagt acgccaccta
      661 cgacgggtac atcacctacg aatacatcga cgacggacag tacgccacct acgacggata
      721 catcacctac aaatacatcg acgacggaca gcacgccacc tacgacggat acatcaccta
      781 caaatacatc gacgacggac agcacgccac ctacgacgga tacatcacct acaaatacat
      841 cgacgacgga cagtacgcca cctacgacgg atacatcacc tacaaataca tcgacgacgg
      901 acagtacgcc acctacgacg gatacatcac ctacaaatac atcgacgacg gacagtgcgc
      961 cgccaacgac ggatacatca cctacgaata catcgacgac ggacagtacg ccacctacga
     1021 cggatacatc acctacgaat acatcgacga cggacagtac gccacctacg acggatacat
     1081 cacctacaaa tacatcgacg acggacagcg gtagtagcga tactcccatg caaatgagtc
     1141 cgcctccaga ctccaataca aatacaagtc cgccagatct tggtaatact acaaagtatg
     1201 atgaaacgtc tggcagttct aaaagcggaa gtggttcaat acctgactca gggtcgtccc
     1261 ccgatgttca acccactcag tttaccccta cttaataagg cttatatcgc tttttaagtt
     1321 atccctccaa caacttgtga ggcaagcttt ctatgcgtcc ggcctgggtg acaatcacat
     1381 aattcaattt tctttgcaat tcgctgatgg agtcggcgcc ggcataggtt agacctgagc
     1441 gcaatccgcc tatgatatca gcaataatcc catcagggtt ttctttaaat ggcacttcag
     1501 ttgcaacacc ttctgctgtt ttccattcat gcatttgtcc aaggaatgct tcctgcgctt
     1561 ctcgggatgc catgcctcga tagcgtttga ctttgcttcc gtcatctttt tgaatgactt
     1621 ctcctggcgt gggggccgag ccagctaaca tacctcctat cattacaaaa tcggcgccaa
     1681 aggccaatgc tttgacgata tcacccgatg ttttgatgcc gccatcagca acgatggaac
     1741 gatcagcacg tgaacaatcc tgaatacagg ttaacatggg tacgccaaag ccggttttaa
     1801 ttcgggtact gcataccgag cctcctccaa tacccgcttt aataatatca gcgccacaag
     1861 acgcaagata atctgctccg gcataagtgg cgacattccc agccataatg cagcggcttc
     1921 ccaataactg acgcaagctt tttaatgttt tgccgacgta tttggcgtgg gcatgtgcca
     1981 catcaacaca aaaaaaatca gctcctgcat cccgcagtgc ttctgctctt tgtaattcat
     2041 tttcagtgca cccaactgat acaaagacag gtcctttgca tttcttaaat tcctgaatat
     2101 tttcttctat agtcataaat cggtgtaaag cgcccatggc tcctttgctg tgcataaaat
     2161 tagccatatt actttcggta atcgtatcca tattggcgct gattaccgga agatttaaag
     2221 tcagtttacc taacctgtcg gtactggttg tttcaacaac tcgtctggat tcatgatggt
     2281 tgtacgatgg aacgagcaga acatcatcaa aagtgatcgc ttgatcagtc attattaatc
     2341 ctcataaaca aatactataa atgctcagcc gcataaaaag ccagccttga gcgctctccc
     2401 cgagtcaaag tcatatgagc gctgtgttcc caacttttaa accggtctac ggcaaaagtc
     2461 agtccggaag tggtttcagt taaatagggg gtatcaattt gcttaatgtc ccccagacaa
     2521 ataattttac tacctgggcc tgctcttgta accaaggttt taatttgttt tgatgtcaga
     2581 ttttgcgctt catcaattat aatataacgg tttaaaaaag ttcttccccg cataaaattt
     2641 aatgagcgta ttttaatttt attttgcagt aaatcctgag ttgccccacg tccaaaactg
     2701 ccgccgactt gcgagctatg aagcacttca agattatcca ttaaagcgcc catccacgga
     2761 gtcatttttt cctcctcggt acccggcaag aatcctatat cctcgccaac aggaatcgtt
     2821 actcttgtca ttaatatctc gctgtagcga ttttgatcca tgacctgggt taatcctgcg
     2881 gctatggtta atagtgtttt ccctgtacct gctgagcctt gcaagctgac aaaatcaata
     2941 tcgggatcca gtaacaagtt tagggaaaaa ttttgttcac ggtttcttgc cgtgattccc
     3001 cagactgaat gtttgggttg agtgtagtca cgaatgagtt gaactacggc attaggtcca
     3061 tcaattttct tgactaaagc ttgaaactgg tcatcttccg tactaaggca atcgtttgga
     3121 ttccattggc ttaccaaagg accgcttacc ttataaaagg ttttaccact ttcttgccag
     3181 gatcccatat ctttggcatg actatcccaa aaattgttat cgagaatatg taatcctctg
     3241 tgaagtaaat tgacgtcatc cagcacttgg tcattgtagt aatcttcagc atgaatacct
     3301 aatattcctg ctttaatacg caagttgatg tcttttgaaa taatgataac ttgtcttttg
     3361 ccataatatt ttttttgcag acctaaagcg gttgcaagta ttgtattgtc tactttatgg
     3421 cccggcagag ttgaaggaac cacttgatca aattcatctg tttgaaaaaa caatcgtcca
     3481 ctgcatttct tgttgctgct attgatgtaa gatgggatag gcagtccaga gacaatttga
     3541 tcgtgagtgg cgttactcat tagctctacc aacatgcggt tcgtttgcct cacgtttctc
     3601 gccaattcgg atgtccctgt tttatgacta tccaattctt caagcaccac cattggcaaa
     3661 taaatatcat gctcatcaaa atggtagata gcagtaggat catgcatcaa aatattagta
     3721 tcaagcacga acagcttaag tccggtattg gtattatcca taggttcacc ctgtcagcta
     3781 aagtattctt caattcaagt ggaatcagtt tcctgcctgg gcagtgagcg cattgaaatc
     3841 cctgaattct atgttcattg tgctgagcta tggtggtccc gtcaagaatt atattgttaa
     3901 gtgtttattt ttaagttaaa tgggtatgcc ttccatatgc cgttctgttc tcaattcctt
     3961 attattgaaa acaatgaacg caatgaaggc cataaaaagt aaaaacaatc caacagggaa
     4021 caggctatta tgattaacca ggcgaccgga ataagtacca aaagcagcga agcacagtaa
     4081 gattaaactg cgaatcgcag aagccgagcc tctgcaatca tcgaagcatt gcagggctct
     4141 ggatgtggtt gcgccccata gcaaggctga acttagaata tagacaccga tagttatggc
     4201 attatagagt tggtacttgc tccagtgaaa gtattctcct attaacaaca tcaggctgct
     4261 cattacagcc aactgaattc ccaatgctat aattttatca atccccaggc gattaaataa
     4321 gcgggaggcc agtaacgatc cgcagacaaa tcccatggaa gtaatcatta ataatatccc
     4381 catggtgtca gcagcgatat gcgcatgcga ataataaaat ggggccactg tcaggaagca
     4441 ccattcacca caatttaata gaccaaatag tgcgatatag gaaataaatg ttttgttttt
     4501 tataattcga atgtaaatgc ctagtgtttc cttcaggtca gcagtcgtcg ttttttgtac
     4561 tgggatggtt ctgggtaaaa acagcataat gacgcataaa atgaaagagt aaaatgcgat
     4621 acaataaaga tttgaacgcc aaccatataa cacatccaat tgtcctccta atgcgggggc
     4681 aaagagtgga atcactgcag tcagtgtccc aatccaggtc atgatgcgag ctccttcgtc
     4741 tttggggaag agatcctgga ctaaagccca acctgcaaca gggacaacga ctgcgccagt
     4801 accttgcaag aatcgacaag ccagtaacaa ctgaattgaa tcggctttta agctgaagat
     4861 tgagcctata aagaaaataa gcatccccag aaataatgtt tttcttctgc ctagccggtc
     4921 ggcaataacg ccccaaataa gaactgtaga aaattgccct atcatgaaaa tggaaatgag
     4981 cattttggct tcatttgatg agacagacag tgcgtgagca atgttaggca ggcttggtaa
     5041 caaaatatct gtaccaatta aaaccagtat gcaagaggcg acaacaataa aaagttgcag
     5101 gttgcggata aacatattga actcttaaga aaagggtgta tttgatgtat ttatccgcaa
     5161 caataatagc aaaaattaat taaatactct ataaaaagta ctgtttcaaa acaggaatcc
     5221 ttatcaggga ttattttttt tatcccacca aagctcgcac tgcgtttaga acttcctcta
     5281 cgtgcccttt aacattcact ttacgccatt cgtgtgtcaa gacaccttgt gtatctataa
     5341 tgaaagtact tcgttctatc cctctgactt gtttgccata catggatttc attttgatca
     5401 catcaaacgt ttggcataat gtttcatttt catcactgat taattcaaaa gggaaattct
     5461 gtttgctttt gaattgttca tgggatttta aactatctct cgaaataccg aaaatcaccg
     5521 tatttaaatc tttaaattgt gagtaggcat ctctgaagtc ttggccttca gtcgtacagc
     5581 ccggggtggc gtctttgggg taaaaataaa gtacgatgat ctgtcctttg taatcattaa
     5641 aatgtccttg cacgttgttc gtggctttaa aagcaaaatc ggggaccatc tcacctattt
     5701 tcataaacca tctcctcaag tgttcttttt cattattctt gaacgatctc tttgccattc
     5761 tctgtctttg atcgtgtctc ttttgtcatg ctcttttttt cctttggcca gagcaatttt
     5821 tattttaatt ttattttttt tccaatacaa agaaagaggg actatggtat agccttgcct
     5881 ttcaacactt ccaatgagat ggtttaattc ttttttattc atcaatagct tgcgcgtcct
     5941 gaccggatca ggaataaaat gagtggatgc ggtgggaaga ggctgtattt gggcgcccaa
     6001 taggaatgcc tcaccgtatt tgattatcac gtgtgaatcc gacaaattga tttttccagc
     6061 acgcagactt tttacttccc agccttccaa aaccaggcct gcttcgtatt gatcttcaat
     6121 aaagtaatca aaaccagctt ttctattcaa agctatggtt gaatctggtt gttttttggt
     6181 agtcataatt catctgattt attgaaaaaa aaacagtata actgatgtac tatcccattg
     6241 aactgctttt cttaattttg ttgccttgaa cggcttatgg caaaaaagta tattctatgt
     6301 tatttctgct ctctttcgta tttataatcc tgaggtaact gtggataaac acgatctaat
     6361 ttttgatcca ctgcaacgcg gtcatcaaaa acaaaacatt tgccttccca aagtgattcg
     6421 ccttcaggaa tagattccag gtaatttaga atgccatcat gaagttggta aacatgctga
     6481 aagcccagct ctttcatata ggctgtcgtt ttttcgcaac gaattccgcc agtacaaaac
     6541 atggctattt tcttgtcctt tttatcaatt aaattgcgct gaacgtagtc tggaaattcc
     6601 ctgaaatttt ctatgtcagg attaattgca ttcttgaaag tacccagttc atattcataa
     6661 tcattgcggg tgtctaataa aataacatta ggatcttgaa tgaactgatg ccattcctca
     6721 gggcttagat aggtccctgc attataagag gggtcaactt tttggacgcc catggtcact
     6781 atctctttac ggagtttcac tttcgcttta tcaaaagggt tcttgttgtc ataggtttct
     6841 ttaaaatgca ggtcggcaaa gcgcgaatca gaacgcaggt aatcataaaa aacattcatt
     6901 tgttctctat tcccggcaaa tccaccgttg acgccctcat gggccaggat gatagttcct
     6961 tttatgccta tttcatgcat tttagtgagt atgggctctc tcagtgattc aaaatcattg
     7021 agaggaatga acttataaaa tgatgcaata ataatatctt tcacgctgta acctaaggat
     7081 taacaaaaaa tcaatttgca aaaattatca tttactcaca atttaatcaa taggaatgaa
     7141 ttaaatgcat ttattggata tcgggatttt aacggcatat tgggtgaaaa atagttgcgt
     7201 tattgcatgt tagtacttaa gtataaagaa tcgttgacaa taattaaatt ctgatcatac
     7261 tctaagggtt aagctgctat cttttgtttc gctagctttg atataaggag atttttatga
     7321 tccaaaacaa caggatttgt cgctggctct tcattgtatt gttgtctctc cctttggttg
     7381 gttttacgtc tattccagtg aaatcaatag tggtgtttgg agatagcttg tctgataacg
     7441 gtaatactac ccatttactt aaaagcctgc gtcaggaaga ggatcctgcg ttcttagtgg
     7501 ctcctttcaa aatttttgtt atcaacaaaa tgatagagtt tgctaacgat tattatgttc
     7561 ctcagatggt acttgacgca ggaatatcag cagtgactga tttttttgat catgatttgg
     7621 ctccctatct tgccaatttg gtgggtaaag taaaacaggt cccggtgctg cctggcaaac
     7681 cttattggaa aaatcgcttt tctaatggtc gtgtctggat tgagtatttg gcagaaatgt
     7741 tatcgataca aaaaagtgat gaagaagtct acctaaataa ggcatttgga ggtagctggt
     7801 ctgccactta tgattatcaa ttaacagttt ggaacttgat acgacatcca ttgggaacaa
     7861 tcaagaacct gatagtagga aaattaattc ctccgagtct tggcctgata gtgcaagctt
     7921 atctgctgga acatgagcaa ttaagtgatg aaacgctcta ttttatttat tcaggaagca
     7981 atgactacat taatgtcctg ttttttgagg acaattacaa tactgaagta atgagcacct
     8041 atattgacaa tgtattggat ggtatgagtt ctgccgttct gaagctggct aatgcgggag
     8101 cgagacgttt tgttattatg ggaataccac atgtggggga tactcccaag tttgtgaaaa
     8161 ctaccgaccg tgacgtgtta aatgcagcgg tagaccttca taatgagcgc cttgcaaaac
     8221 ggattgacgc atggaaagag cggtatccaa cagccgactt cttgtttgtg aatacagagc
     8281 aatatctgaa aaaagcgttt gaagatccgg agaaatatgg tttttataat gtgaaagatg
     8341 cctgtatcga tgtgaagttc cccatgttca atgcattcat tcactctcca tttgctcgta
     8401 actacgtatt gcaatacaca caggttcttc aatatcgaga taagcaattt gctccaggtg
     8461 aaaataacta tcatatgtgt agtgagccag aagactattt attctgggat gaaattcatc
     8521 caaccacgag agcacataac tatcttgctt atgaggtatg tatggctttg gaggaacatg
     8581 gctacgaggt aacttgcaaa atgccagagt ccgcctaata taattaggta tcatcacctg
     8641 aaccacatta acagtggttc aggtgatttc atgatgaagt tgtcaaaatt gatcctcatt
     8701 taagtaaatc cagagtggtt tttaagcaat tcttgagtct ctaaatgatg acgaagatta
     8761 atttgcctta gtgatttgta aaactttatg tcataattcc ccttttttat tgatatactt
     8821 cattcatatg agggaatatt atttataaaa tttaaggcac cggttattta ggaaattagc
     8881 tgcattctta tgcgtttcac tctttatcta tatatagtag atgaaccgag aatgaaaaag
     8941 cttatttcta caaaaacagt tgctgaaaag aggagcaggt tatgtgcggg attatggggg
     9001 ctgtttcaga acgggatatt agcaagattt tattggaagg cttgcgccga ttggaatata
     9061 gagggtacga ttctgcaggt attgccgtca tagatagtca agatcgttta aaacgagtaa
     9121 gaattcaggg aaaagtacag aatttggcgg atgctatgca agagaccgct atcgctggaa
     9181 atacaggtat cgctcatact cgttgggcga cgcatggtaa accctccgaa caaaatgctc
     9241 atcctcattt atctcatgga gaaatcgcat tagttcacaa tggcattatt gaaaatcatg
     9301 aacatttgcg tcaacaactt attacttatg gttatcaatt cacatctgaa actgatacag
     9361 aggttgctgc gcatttaatt cattatcatt accagcagca tgagaattta ttacttgctg
     9421 tacaaaaatc tgctgcagaa atgcaagggg cttttgcatt aggagtgatt catcagaaaa
     9481 gaccagaaga attggttgca attcgtaaag ggagtccttt ggttttggga tttggaattg
     9541 gtgagaattt cattgcctct gatgccttgg cactcagatc tttcgcacaa tcagttattt
     9601 acatggaaga aggtgacagt gcttgtgtca caacacagga tattaaggtc tatgattcca
     9661 atcgaatact tgttcaaaga gcggctcatc cgttgaacag tgattctgag atagtgaata
     9721 aagggccata caggcatttt atgctgaaag aaatatttga acaatcgaag gtaattacag
     9781 acacgttaga aagtcgaatt aatagcattg atgtgctaag agcgagtttt ggtgagaaag
     9841 catcccatat ttttccaata gtaaaaaata ttcacattgt agcatgtgga accagttatc
     9901 atgctggaat gattgccaaa tattggcttg aatcacttgc cggcctacct acacaggttg
     9961 aaattgcaag tgaatatcga taccgagatg ttgttgtccc cgacaatacc ttatttatca
    10021 ctgtatctca gtcaggagaa acagctgata ctcttgctgc cctatttaag gcaaagcaat
    10081 cgaattattt ggctagttta gctatatgca atgttgcaac cagtacttta gtgagagagg
    10141 cggattgtgt ttttttaact cgggcaggta ttgagattgg tgtagcatct accaaagcgt
    10201 ttactacaca gttggcagcc ttcctgatgt tggcagcagc actttgtaag gacaatcgtg
    10261 cgcaggaagt tttaagacaa ttacaagagc tgcctgcctg ttgtgagcgt gttttgcaga
    10321 tgaatgaaga agtcgaatca ttggcctctt tgtttgtaaa taaagttcat gccttgtttt
    10381 taggtcgagg ggttcaatat cccgtagccc tggaaggtgc attaaaactt aaggaaatat
    10441 cttatataca tgcggaagct tatcctgccg gtgaattaaa gcatggtcca ttggctttgg
    10501 ttgataagga tatgcctgtg attgcagtag cccctaatga tgagcttttg gataaattaa
    10561 aatccaattt gcatgaagtg agcgccagag gcgggcagtt atttgtattt gttgatgatt
    10621 cacaaaattg gaaagcgaat ggtgctcgtt taatcaaagt tccatcctgt ggtgcttggc
    10681 ttgcgcctat tatttacact attcctttgc aattgctggc ctatcatgtt gctgtagcta
    10741 aagggactga tgtggatcaa cctagaaacc ttgcaaaatc ggtgactgtg gagtgaacaa
    10801 aggccaatat ttttggaatg agttatggtg cgagggacgt atttcttttc ataaaaaaga
    10861 agtaaatccg gatttgatag cctatgtctc ttccttaaat atacctgcca agggaagggt
    10921 tctggttcct ttatgtggaa aaagtgtcga tatgctttgg ttagtccgcc aagggtatca
    10981 tgtggttggt atcgaactgg ttgaaaaggc tattcttcag tttgtccagg agcaccaaat
    11041 cactgttcgg gagaatacga taggtcaagc aaaacaatat tttacagata atttgaattt
    11101 atgggttacc gatatttttg ctttaaactc ggctttaata gaaccagttg atgctatcta
    11161 tgacagagca gctttggttg ctttgccaaa aaaattgcga ccagcgtatg tagatatttg
    11221 cctcaagtgg cttaagcctg ggggaagtat tctgttaaag actttgcaat ataatcaaga
    11281 gaaagtgcag ggtcctccgt atagtgtttc cccagaagaa atcgctttat cgtatcagca
    11341 atgtgccaaa atagagttgt tgaaatcgca aaaacgaatt caggagccta atgatcattt
    11401 atttaatttt ggtattagtg aagtgaatga ttctgtctgg tgtatcagaa aggggtaaaa
    11461 cgcagtacag tgttacgcat aaggacaatt tgtttatata ttagcttaat ttctatatcg
    11521 cattatagta tctgatgagt atataatgag acgattttta tcatgtttta cggggatatg
    11581 aatggcagaa gtgatgttgg cgtcagccgc aattattgca gatgaactta aggtcaaagt
    11641 atcgcaggta gaaactgcta ttcggctgtt agatgaagga gcaacggtac cttttattgc
    11701 gcgttatcgc aaagaagcaa ctggagggct tgacgatgtg caattgcggt ttcttgcgga
    11761 aaggctaatt tatttgcgtg aattggatga gagacgaaca gtcattttgc aatccattcg
    11821 tgaacaagaa aaattaacac cggaattgga acaaagcata ttagctgctg aaactaaaac
    11881 acgcctggaa gacttgtatt taccctatag gcctaaacgt cgtacaaaag cacaaattgc
    11941 tattgaagca ggattagagc cattagctca tgctttatgg aaagaccctg ctttagatcc
    12001 tgaagaacag gcttcccaat ttataaatag cgaagcaggg attgaaaata ctcaggccgc
    12061 cctggatggt gcgcgacata ttttaatgga gcattttgct gaagacgccg agttaattaa
    12121 cgaattgcga gagtatttgt ggcaacacgg aatagtcaaa tcagtaggaa acagtactaa
    12181 aaaggatcag gcgaataaat tttctgatta ctttgattat tcggaagcga ttaaaaaaat
    12241 tccatctcat cgggcactgg ccttgttccg ggggcgacgt gaaggaattc tgcaagtaag
    12301 cctggtttta cctgatcagg atgccaatta tggagagaat aaggttgctg cttactttaa
    12361 tgtcactgac aggcaaagaa aagcggatgg ctggcttata gatacggtca ggatgacttg
    12421 gaaaattaag ttatttacca agctggagct tgagttatta actcgtctgc gcgaaatagc
    12481 agatgaggaa gccattaaag tgttttccaa aaatttacgt gatctgttgc tggctgctcc
    12541 cgcaggtcct caagtgacga taggtcttga tcctggaatt agaacgggtg ttaaagtggt
    12601 tgtcgtggat gctacaggta agctgttgga ttactctgtt atttttccat ttgctcctca
    12661 aaatgaatgg catcaatcca ttgcggattt ggccaagttt gccgcgaaat atcaggttaa
    12721 tttaatcagc ataggaaatg gcaccggttc tcgggaaaca gagcgattgg tagctgattt
    12781 aattaaaatg taccctgatt tgaaattaac aaaaataatt gtcagtgaag ccggggcatc
    12841 tgtttattcc gcatctgaaa ttgcagctaa tgaatttcct gatttggatg tttccctaag
    12901 aggcgctgta tctattgcac gaagattgca agatcccttg gcagagctgg taaaaattga
    12961 agccaaatca ataggtgttg gccagtatca acacgatgtg aaccaggcaa agttggcgcg
    13021 ttgcctggat ggtgttgtcg aggattgtgt gaatgctgtt ggcgtagaca ttaatacagc
    13081 ctctgcagct ttgctatccc atatttctgg attgaatgat actttagcta aaaatattgt
    13141 gcaatttcgc gatgaacatg gtgctttcac aaacagaaat caattaaaaa atatcaatcg
    13201 catgggagaa aaggcttttc agcaagcagc cggctttttg cgcattatga atggagataa
    13261 tgctttagat gcctcttgtg ttcatcctga agcctatcct ttagtggaaa aaattatttc
    13321 agataaaaaa gttagtatta aggaaataat cggcaataga gaattattgc agagtattaa
    13381 cgcggaacaa tatgtggatg agaattttgg tttgccaaca ataagagatg ttttacgtga
    13441 attggaaaaa ccgggccgag atccacgacc agaattcaga accgctcaat ttaaggaagg
    13501 tgttgaggac attaaagatt tggaacaagg aatggttctg gaaggtgttg taagtaatgt
    13561 gactaatttt ggtgcctttg ttgatattgg cgttcaccag gatgggttgg tacatatttc
    13621 agcaatgacc aatcgcttta ttacagatcc tcgttctgtt gttaaagccg gtgatattgt
    13681 aaaagttaaa gtggttgaag tggataaaga acgccgaagg attggtttaa gtatgaagct
    13741 gaatgatgag caggctcctg ctgtattgca gaaagctaat aaaccacagc cggcaagaaa
    13801 acaagcggaa caaaagcagc ctgaatttaa gaagaagcaa gaacataaga aaaaaccaga
    13861 taagataaaa aaatcggagc cagtcaaaaa aaccgtattt aatacagcga tggccgatgc
    13921 tttagccaaa ctaaaacgtg gtggagaata gggatgcaaa cacctaaagg agagttgact
    13981 atccaaactt tggccatgcc tttaaatact aatgccaatg gcgatatatt tggcggatgg
    14041 atagtttccc aaatggattt ggcagcaggt gttttggcaa agagaatttc ccatggacgt
    14101 gtcgctaccg ttgcaattaa ttcaatgact tttttaaaac ctgtacatgt aggcgatgta
    14161 gtgagctgcc acgttgagtt agtgaaagtg ggtaatacct ctatgactat tcgggttgag
    14221 gtatgggctt actcatcgat caaaatggaa aaataccagg ttactgaagg gatatttgtt
    14281 tttgttgcga ttgatgaaaa tggaaagccg aggcaggtac caaaaacaca atgactaata
    14341 aaactcaaga gttgcagcga gtaattgctg atatggctgt attgattgag caagatgcag
    14401 agcctgaccc tcaattgtat gccttttttt ttcaacatcc ggaaattagt cttgaattaa
    14461 tacaccttat taataatttg agtgaagaat tagtgcatga tgggcctcca atatactcag
    14521 catgcgtttt cgctttagac atttgtattg ctcaattgca agcagctgca gaggcaaata
    14581 ataaaaatgc cacaaaatta ttgtctcaac taatgaatca tttagctgag gtcattgata
    14641 cacataagca tagcttaagc ttttggttac ccgtactgaa tgcattttat gaagtgcatg
    14701 ttgagctaac tgacgattta aaggaagcat attatggctt ggcaagccag gatgatgatg
    14761 cggtagaagt ggatgagcaa tttaatcatt tggactccat aaaagatttg atccatgaat
    14821 tatctgattt aaccctgttt gacatagcag agagtttttt tgcacaaagc tatgccatgc
    14881 cgcctgattt ttttgcagat ttgattgttg atttgtatag tattgaagaa ggtaaagata
    14941 ttgcactact ggttttactc catcctaata tcgaggtacg agagattgcg ctgtctacta
    15001 tcgatcaatt aatgaataca attactttga cgccagcatc actgtcaaga ttacaaataa
    15061 tcaaacattg gtatccgact cattaccatg gaatgtttga tcgttggatt aaaacgcaaa
    15121 gaaaaaaggg agtaacattt gaaacagagg ctagtcaagc aaaaataacg attaaagcaa
    15181 ctgaagtgga tggcagtgga tcacaaggag tttttatcca cgcaaggaaa aataaaaaaa
    15241 acaggttatg cggcttatta ttaaagtatg atttgggcat taaagatgcc tggataactc
    15301 cagagatttc tgcagcggaa gttacagatt attatcataa agcgtttgaa gacagcgtga
    15361 ccttgcgtga agttgatttc tcttattttc aaacaatagc ggagcatttt ttagccatta
    15421 ctgttgaaaa aagtgatata cctaatttgc attttttaga aatactcgaa tttctgggca
    15481 ttcgtctgaa acctgataag cttgatttgg attgcgtatt tgaacaatta agtgttcaga
    15541 ttattccttt tactcaagaa aaaatagcag aatctttaaa acgctcgaaa atgtggctta
    15601 aaaataagca atttacagaa tcatggtata ttgaaaatcc caccattgat aaaattgtca
    15661 atcataacag cagctttgtg gaaggagtta aagtttgtcg tttggctgat gctatggacg
    15721 cggtattttc cgaggaaatg gaattacacc gggataaatg gcagtttcat tttttatgga
    15781 tagcgttatg ggttaaggca aaacacagaa aaaatgaaaa ggtttggcag gacagttttt
    15841 tgatagctta tgctattcat caaggatatc cattgaaaga tattccagta atgaaagaga
    15901 tatgttatca aacagttgta aacagcatag aaaccatgca ggaaagaaga acacacttga
    15961 gccaggaatg aataggaaaa tgttccggaa ttattcttgt taataaacta ggcaacgtcc
    16021 ctgaagtttt tttagacaag atttaatcat gaattgagct cggattttca acttcaccag
    16081 gatcttcccc cctctcccgc gctaggagct ttgatagtag gatggtgcct ttcgcgggag
    16141 agggttgggg agagggaaat ggacaaatct acgctaagac aacgagctcg caatttgcgg
    16201 caaaatagca cggatgccga gagacatctt tggtatcatc ttagggcaaa tagactggga
    16261 tttaaattca ggagacaagt gctcataggt tcatacatcg tcgattttgt ctgtattgaa
    16321 aagcggttga ttatcaagct tgatggcggt ca
//
LOCUS       DBNCWK010000062        16251 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.062, whole genome shotgun sequence.
ACCESSION   DBNCWK010000062 DBNCWK010000000
VERSION     DBNCWK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16251
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.062"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(9..1193)
                     /locus_tag="AC06IO_001736"
     CDS             complement(9..1193)
                     /locus_tag="AC06IO_001736"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIO9874915.1"
                     /translation="MTDVYVVDVLRTPVGKAPRGVFKNTLPDDLLAHTIKCLIHRHQN
                     IDWQEIADVVIGCAMPEAEQGMNVARIASLLADLPQSVPAMTINRFCSSGIQSIVTAA
                     DTIRNQEAHLALGGGVESMSMVPMGGNKYTANPSIFNDEHVAIAYGMGITAENVAQRW
                     EITREQQDEFAIESHKKAIAAQERLDFNAEISPIEISIRYPDLKTSKVEIKKKLINKD
                     EGPRADTSYEAISKLKPVFALNGTVTAGNSSQTSDGAGVALLASEKALKQYDLKPLGR
                     LMSYAVAGVPPEIMGIGPVKAIPMALKRAGITLDQLDWIELNEAFAAQALAVIKDLDL
                     PREKVNPLGGAIALGHPLGATGTIRTATLLHGLRRTKGRYGMVTMCIGTGMGAAAIFE
                     AL"
     gene            complement(1209..3578)
                     /locus_tag="AC06IO_001737"
     CDS             complement(1209..3578)
                     /locus_tag="AC06IO_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIO9874916.1"
                     /translation="MQEPFFIKKVAVLGAGVMGAQIAAHCVNAGIETLLFDLAGKEGP
                     KNSLVDKAIANLGKLKPTPLATPQTNELLQAKNYAENLEELSSCDLVIEAIAERIDWK
                     EDLYKRISPYLSDKTILVSNTSGLSINKLCEVLPAQHRSNFCGVHFFNPPRYMHLAEL
                     IPANTTSSDLLDYLETWLTEYLGKGVVRAKDTPNFIANRIGVFSLLTTLHHTLAMNMG
                     IDEVDALTGPLLGRPKSATFRTMDVVGLDTMQHVINTMQSQLTGDPWHKMFILPDWLN
                     GLIKEGHLGQKSGQGIYRKAGKVIEVYDIQTGQYRPSRAEVSDELKAIMDIKDPEARM
                     KNLMTSNNKQAQFLAACFRDLFHYCAYHLEEIADNARDVDLAIRWGFGWSQGPFETWQ
                     LSDLHSVTRQIDESIQNNKALSSAKLPEWLYEIKEFYTREGAYSPGKNNYHARSQLPV
                     YHKQFFHDRVLKESHHPVHVIYENEGVCLWYLKDDVAVISFKSKANTIGQSVLDGIET
                     ALDIAERDYQGLIIYQQDASNFSSGADLRGVSKLLTDGTIQALEHMVEQFQRVAMRLK
                     YSSIPTVAALRGRALGGGCELMMHCDSIVAAFESYPGLVEAGVGVIPAGGGCKELAMR
                     SAHHAQQADLLTFILPVFQQIATAKVAASAVEARNMGYLRTADSILMHADEVLYASLE
                     KIKAMRAANYLPPMKKQRFKVAGIEGHARLQSGLVNWLEGGFISQHDYFLANELAKVL
                     CGGDVNHGTLVDEQWMLKLEREAFIKLANTSLTQARISHLLETGKPLRN"
     gene            complement(3698..5218)
                     /gene="amaB"
                     /locus_tag="AC06IO_001738"
                     /gene_synonym="amaA"
     CDS             complement(3698..5218)
                     /gene="amaB"
                     /locus_tag="AC06IO_001738"
                     /gene_synonym="amaA"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-piperidine-6-carboxylate dehydrogenase"
                     /protein_id="HIO9874917.1"
                     /translation="MDLLQRLNIKSVNPGAFSGHGWHSDNHAHTLESFNPSTGNKLAE
                     IATCTMDDYEQVMQRAEQAAQTWRKVPAPKRGEIIRQIGQALRENKDSLGSLVSLEMG
                     KSKQEGDGEVQEMIDIADFAVGQSRMLYGNSMHSERPNHRMYEQWHPYGIVGVISAFN
                     FPVAVWSWNAFLSAICGNVTIWKPSAKTPLCAVAVQHICNQVLKENNCPEIFGLIIPN
                     SHDVVEAMVDDKRIQLISFTGSTAVGKQVAAKVAARLGKSILELGGNNGIILDESADL
                     NLAIPAIVFGAVGTAGQRCTTTRRLFVHESKYQDVIKRLRHAYEQITIGDPLDTRNLM
                     GPLIDQQAVEQFKKAINRIKAAGGQIVYGGEILKQAGSFVQPTLVCDVKNDWDIVQEE
                     TFAPILYVMSYRTLDEAIALHNGVPQGLSSALFTQNLKNAELFLSACGSDCGIANINI
                     GTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINWGDELPLAQGIRFNLS"
     gene            complement(5228..6316)
                     /locus_tag="AC06IO_001739"
     CDS             complement(5228..6316)
                     /locus_tag="AC06IO_001739"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIO9874918.1"
                     /translation="MYNVMITGAGKIGSLIACLLADSGSYQVHLADLEFNGSDVTRLL
                     TALPEIKTVALDVKDEQSTQAYLQKHNIIAVISSLPYFLNTHVAKAAKAAKAHYFDLT
                     EDTSVTEAVKAIAQNAETAFVPQCGLAPGFISIAANSLMQEFEKCHHARLRVGALPQR
                     ANNALHYSLTWSTDGVINEYGNPCYGIEGGKDVVMAPLEGLESIQIDGCEYEAFNTSG
                     GLGSLAELYAGKIQSMNYKTMRYPGHCKKMRFLMNDLRLNEDRGTLKRILENAIPKTY
                     QDIVIVYVTVEGIKQGELTEKSYVKKIYPEVIRGLEWSAIQVSTASGVCAVVDLVLGQ
                     GNEYKGLILQEKFKLSHVLENRFGKYYA"
     gene            6470..8005
                     /gene="lnt"
                     /locus_tag="AC06IO_001740"
     CDS             6470..8005
                     /gene="lnt"
                     /locus_tag="AC06IO_001740"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIO9874919.1"
                     /translation="MKQAALIHSTIINTSTLNQYSKFVVVFLAGLLAPLGFAPFHLPG
                     LTILSLAILFSEFLQCTVKQGFTLGFLYGLGYFGFGVSWVIISIHDYGQLDYFLAGVI
                     TLLFIMYLSLFPGIVGYSFKLLSTKCNLLTCMLLFSTLWCIGELIRSKLFTGFPWLLT
                     GITQIDTPLRYIAPVVGVYGLGLICSFLGTLLVLIIKDNTIKRYYYLSVFTLILIAPS
                     LGKNIHWTEIKREPISVGAIQPNLSMRDKWDETLFWDLLKYYENKTDKLLGKNLIVLP
                     ESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNSIISLGEATGAY
                     VKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLMNIANHPIASLICYEIAYP
                     NILRSQMPAAQWIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNNDGLSSVI
                     NHQGIIVEGLPAFNSGILQTDIFPANGTTPWIIWSDYPAFVFCFIFVLFVLFFNIKRF
                     TEK"
     gene            8071..10542
                     /gene="leuS"
                     /locus_tag="AC06IO_001741"
     CDS             8071..10542
                     /gene="leuS"
                     /locus_tag="AC06IO_001741"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIO9874920.1"
                     /translation="MDNTYNPQEVEEQAQQYWHKKQSFNVTEDLNKEKFYCLSMFPYP
                     SGTLHMGHVRNYTLGDVIARYQRALGKNVLQPIGWDAFGLPAENAAIKNKIPPAEWTR
                     KNIAAMKEQFLRLGNAYDWKREITTCDPEYYRWEQWFFIRLFEKGLVYKKNAVVNWDP
                     VDQTVLANEQVVDGRGWRSGALVERKEISQWFIKITSYADELLSSLDSLDEWPAQVKQ
                     MQRNWIGKSIGTEIYFNVNNYPKRLKIYTTRPDTLMGATYLAVATDHPLAKEAASNNK
                     KVQEFLDSCQGIKIAEAELATMEKRGIDTGMTAIHPITGKELPIWVANFVLMQYGSGA
                     VMAVPAHDQRDWEFAQKYQLPVKQVIKPIGIEHDFNQSAYTEEGILINSNQFDNLLSS
                     KAIQVITNFLEENDAGKATINYRLRDWGVSRQRYWGTPIPMIICEQCGIVPVPDEELP
                     VVLPENVDFTGTGSPLTQCKEFVNVTCPKCGQDATRETDTFDTFVESSWYYARFACKG
                     QENAMLDDRAKYWTPVDQYIGGIEHAVMHLLYARFFHKLMRDEGLVNSDEPFKALLTQ
                     GMVLKDGHKMSKSLGNVVDPNHLINTYGADTARLFVMFASPPEQSLEWSDSGVEGAHR
                     FLKRVWAFSHQHRDMLIDINDSILSGNGHVDWKEAESRLKKSRHIVHQILAQATHDYD
                     RNQFNTVVSGCMKLFNEISDYSIETENDKFFIHSSISILLRLLAPITPHICHCLWQQL
                     GFDKAIIDAPWPKVDKSALKTDEVDYVVQVNGKLRAQFTASTDATEEELIAAAKEHAH
                     NFVVNHTIKKAIIVPHRQLINLVIG"
     gene            10643..11134
                     /gene="lptE"
                     /locus_tag="AC06IO_001742"
     CDS             10643..11134
                     /gene="lptE"
                     /locus_tag="AC06IO_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HIO9874921.1"
                     /translation="MTQIHKYLASLILIWLLTGCGFHLRGAIDIPEWLDNVSIISRDG
                     NKELVSILKSQLEGYKIDVNSDPSLAKYWLIINSSSLQQQIVSIGASTNPRQYQLILT
                     IEFMLQTPKGQIIKTPRKVNVSRQLTVNNDRILGSNEEQALLVSEMRRDAAIQIINRL
                     NRP"
     gene            11136..12161
                     /gene="holA"
                     /locus_tag="AC06IO_001743"
     CDS             11136..12161
                     /gene="holA"
                     /locus_tag="AC06IO_001743"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIO9874922.1"
                     /translation="MQINQQALTQLLQKKIHHLNVLIGQDHYLIDEALNKIKSMLKKS
                     SECDEKILSIQSPEEWHVVLEETNNYSLFYQTVILTIFFDKKTIDTMGKKILGEYLKS
                     ANSHSYIIIRAPNLTAKQLQWLTHDEQALIIIAQPLSSEAMKRWINEQLKLNTISFDP
                     YVPELIYQYTKGNMLATAQVIEKINLSNTPSTLITPYLALEHLSDQCDFSLFELIDSC
                     LLGHSDRAIHILRQAANNKTEVTLVLWMLTQEIRTLLQLLFLMQQKMDIKMASNQLKI
                     WTQRVNLYQSAVKRLNTLLLQQLHHYCQSIDDKIKSNSNNQVWNALENISLSLCLGQL
                     LGDACIA"
     gene            12149..12784
                     /gene="nadD"
                     /locus_tag="AC06IO_001744"
     CDS             12149..12784
                     /gene="nadD"
                     /locus_tag="AC06IO_001744"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIO9874923.1"
                     /translation="MHSIAIFGGTFDPVHNGHIKTSLAIQANFGFDSYYFLPCKSPAI
                     KPPSFASSEQRVEMLKLALKPYPDFKIDTRELDRDTPSYMVYTLQSFRQEYTDSSLTL
                     IIGYDGLLNLPQWYQWEKIISLANLLVINREEFFQQPVPKSVQTLLNQYRNDDKNILL
                     NHHAGSICLYNAGHYDISSTKIREQLKQHKDVKNNLPDLVYDYIKKQELYQ"
     gene            complement(12759..13292)
                     /locus_tag="AC06IO_001745"
     CDS             complement(12759..13292)
                     /locus_tag="AC06IO_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIO9874924.1"
                     /translation="MQLQWVDIAIVAIIALSVLTGLFRGFVKELVALCVWILAIWLGF
                     NYSQSLDPWLSSYIEEQSVRTAIGFIIILFATLFVGGVVNAILSFILKRAGLSGTDRT
                     LGMGFGFLRGVFIVALIMVAVKMTSLPYQEYSQKSKLYARFDPVVALLYSHLPEFIKQ
                     VKTVDKTENIIDTVPAS"
     gene            complement(13295..14065)
                     /locus_tag="AC06IO_001746"
     CDS             complement(13295..14065)
                     /locus_tag="AC06IO_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIO9874925.1"
                     /translation="MKLIMDEKVKHRLIGLAVIISLGAIFAPALIKKSSQSPESNFSV
                     NVKLPPKPLPPDVVITDEKEVFKTIKVAKTEIPPIPVEKKSINLAKAEPISTEEDNLI
                     RTSKVATLEAERENSIKSEPIKLAVNKAANATAKKVMTLAAAKPPVKTSPIVTAKVNT
                     NKKVSQIVAARKPVVKQDIYAVQLASFTQIANAQALVNRLRSKGYKANYLKSNSRNGI
                     VYKVVVGHSPVKKDVIKLKTQLASAMQLNGFVVNTGVS"
     gene            complement(14062..15348)
                     /gene="folC"
                     /locus_tag="AC06IO_001747"
     CDS             complement(14062..15348)
                     /gene="folC"
                     /locus_tag="AC06IO_001747"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIO9874926.1"
                     /translation="MHTPWSEWDLSHWLTHLETRNTREIQLGLTRVLTVAQKLNLQRP
                     NCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTSPHLLAFNERIRINLAPIQDKDL
                     CQAFSVIEEARYPTNLTYFEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDL
                     AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEFAVQLGASSY
                     LYGKDYFYQEEADHWGVTCQGKPFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNL
                     KKAMELIYIPGRLQLEKGKTDILYDVSHNPQSVRLLADTVKKINVKGQIHAVFSALKD
                     KDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMA
                     LKQASDDDLIIVYGSFFTVSHVMAAQHNLLEQKENQ"
     gene            complement(15329..16213)
                     /gene="accD"
                     /locus_tag="AC06IO_001748"
     CDS             complement(15329..16213)
                     /gene="accD"
                     /locus_tag="AC06IO_001748"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIO9874927.1"
                     /translation="MSWLKKLLPSRIRTETSQKKGVPEGLWVKCSGCNEVLYSTELAK
                     NFMVCPSCNFHHRISARLRIAQFLDESGQEEVAAGLEPQDRLKFRDSKKYKDRISQAQ
                     KATGEKEALIVVKGFLMQQPVVVSAFEFNFMGGSMGATVGEKFVRAVNVAATEKRPYI
                     CFTASGGARMQEGLFSLMQMAKTSAALAKFAETGLPFIVVLTDPTMGGVSASFGSLGD
                     IIIAEPNALIGFAGPRVIEQTVRQTLPEGFQRSEFLLEHGHIDMIVERKHLRATISEL
                     VSKLTHKGAESFFAHAME"
BASE COUNT         4937 a         3468 c         2846 g         5000 t
ORIGIN      
        1 tatatcttct ataacgcttc aaatattgca gcagctccca ttccggttcc aatacacatg
       61 gttaccatac cgtagcgccc ttttgttctt ctcaatccat gtagtaatgt agctgttctt
      121 atggtgcccg ttgctcctaa aggatgtccc aaagcaatag ccccacctaa agggttaact
      181 ttctctctgg gtaggtcaag gtctttaata accgccaaag cttgggcagc aaaagcttca
      241 ttcaattcaa tccaatccaa ttgatctaaa gtaattccag cgcgctttag cgccatagga
      301 attgccttta ctggtccgat tcccatgatt tcaggcggta ctcccgcaac ggcatagctc
      361 attaaacggc ctaaaggttt taaatcatac tgttttaaag ccttctcact ggctagtaat
      421 gccactccgg ctccgtcact cgtctgagaa ctatttcctg cagtcactgt cccgtttaaa
      481 gcaaataccg gttttaattt agaaatcgct tcgtaagatg tgtctgctct agggccttcg
      541 tccttattta tcaatttttt ctttatttca acctttgaag ttttcagatc agggtatctt
      601 atagatattt cgattggact tatttcagca ttgaaatcga gtctttcctg cgcagcaatt
      661 gcttttttat ggctttctat ggcgaattca tcctgttgtt ctcgagtaat ttcccaacgc
      721 tgggcaacgt tttctgctgt aatccccatg ccataagcta tagcaacgtg ctcatcatta
      781 aaaatggatg gattcgccgt gtatttattt ccacccatag gtaccatgga catgctttcg
      841 acaccgccac ctagtgctaa atgagcctcc tgattacgga ttgtatctgc tgcagtaact
      901 atactttgta tgccagatga gcaaaaacga ttaatagtca ttgctggaac agattgtgga
      961 agatcagcca ataaagaagc gattcttgct acattcatac cttgttccgc ttctggcatt
     1021 gcacaaccta ttactacgtc agctatctct tgccaatcaa tgttttgatg tctatgtatt
     1081 aaacatttaa tagtatgagc caataaatca tcaggtaatg tgtttttaaa aacacctctt
     1141 ggtgctttac ctactggtgt acgtaatacg tcaactacat atacgtcagt catcctaatt
     1201 ctccttgctt agttgcgcag tggtttacca gtctctagca gatgactgat tctggcttgg
     1261 gttaatgatg tattggctaa ttttatgaat gcttctcttt ccaactttag catccattgt
     1321 tcatcaacca aggttccatg attgacatcc ccaccacaca acactttcgc caattcattc
     1381 gccagaaaat aatcatgctg tgatataaat ccaccttcca accaattcac tagaccggat
     1441 tgtaatcggg catgcccctc tatacctgcc actttaaatc gttgtttttt cataggagga
     1501 agataattag cagctcgcat ggctttgatt ttttctaaag aggcatataa aacctcatca
     1561 gcatgcatta gaatagaatc agccgtgcgc agatatccca tatttctggc ttccaccgca
     1621 cttgcagcta cttttgcagt tgctatttgc tgaaaaactg gcaatataaa agttaataaa
     1681 tcagcttgtt gggcatgatg tgctgaacgc atagcaagct ctttgcaacc accaccagca
     1741 ggtataactc cgactccagc ctcaactaaa ccgggataag attcaaaagc agcaacaata
     1801 gaatcacaat gcatcattaa ttcacaacca cctcctaatg ctcgacctct taaagcagct
     1861 acagttggaa ttgaactata ttttaagcgc attgctaccc gttgaaattg ctctaccata
     1921 tgctcaagag cttgtattgt tccatctgtt aataatttgg agacgccacg tagatcagct
     1981 cctgaactaa aattggatgc atcttgttga taaataatta atccctggta atccctttct
     2041 gcgatatcca aagcagtctc tatgccatcg agaactgatt gaccaattgt attggctttg
     2101 cttttaaaac taatcacagc cacatcatct tttaagtacc acaaacaaac accctcattt
     2161 tcataaataa catgaacagg atgatgtgac tcctttaata ccctgtcatg gaagaattgt
     2221 ttatggtaaa caggtaactg acttctggcg tgataattgt ttttaccagg cgaataagcg
     2281 ccttctcgtg tgtaaaattc ctttatttca tataaccact caggtagttt tgcgcttgat
     2341 aatgccttat tattctgtat tgattcatcg atttgtcgag tcacactgtg taaatcagaa
     2401 agctgccaag tttcaaatgg tccttgcgac catccaaatc cccagcgaat tgccagatca
     2461 acatctcttg cattatcagc aatttcttcc aaatgatacg cacagtaatg gaataaatct
     2521 ctaaagcaag ccgccaaaaa ttgagcttgc ttattatttg atgtcattaa attcttcatt
     2581 cgagcttcag gatctttaat atccattatc gctttcagtt catcactcac ttctgctcga
     2641 gagggtctat attgaccagt ttgaatatca taaacctcta ttactttacc tgcttttcgg
     2701 taaattcctt ggccagattt ttgccctaaa tgaccttcct taattaaacc attcaaccag
     2761 tccggcagaa taaacatttt atgccatgga tcaccagtta attgagactg catggtatta
     2821 ataacgtgtt gcatagtatc caaaccaacc acatccatgg tacgaaaagt tgcagatttc
     2881 ggtcttccta ataatggacc agtcaaggca tccacttcat caattcccat attcatagct
     2941 aaagtatgat gcaaggtagt taatagtgaa aagactccta ttcgatttgc gataaaattg
     3001 ggagtgtctt tcgctctcac aactcctttg cccaaatact cggttaacca tgtttccaaa
     3061 taatcgagca aatcagatga tgtggtattg gcaggaatta gttctgccaa atgcatgtaa
     3121 cgcggtggat taaaaaaatg gactccacaa aaattggacc tatgctgcgc aggtaatacc
     3181 tcacatagct tattaatact tagccctgat gtattactaa ctaaaattgt tttatcagat
     3241 aaataaggcg aaattcgctt gtataagtcc tctttccaat ctatacgttc cgctatcgcc
     3301 tctatgacta aatcacaaga agataactct tccaaatttt ccgcgtaatt ttttgcttgt
     3361 aataactcat tggtttgggg agttgcaaga ggagttggtt ttagtttacc taaattggca
     3421 atagctttat cgaccaagct gtttttaggc ccttctttgc cagctaaatc aaacagtaaa
     3481 gtctctattc cggcattaac gcagtgtgct gcaatttgag cacccatgac gccagcccct
     3541 aaaaccgcaa cttttttaat gaaaaaaggc tcctgcattg ttattatcct cttgttaata
     3601 atcgattgat gcctctataa gtgagattta caggtaacag gcacttacca ttggctaaaa
     3661 ggagcaataa tctgttgacc ttgctccttt ctacatctta acttaaatta aaacgaatac
     3721 cctgagccaa gggcaattca tctccccaat taattgtatt ggtttgcctc ctcatatacg
     3781 ctttccagct atcagagcct gattcacgtc cgccaccagt ttctttttcg cctccgaaag
     3841 cacctccgat ttcagcccct gaagtgccaa tattaatatt ggcaatacca caatcacttc
     3901 cacaagcact gagaaagagc tctgcatttt ttaagttttg ggtgaataag gctgaagaaa
     3961 gcccttgagg aacgccatta tgtaaagcaa tagcctcatc cagtgtccga taggacataa
     4021 catatagaat aggagcaaaa gtctcttcct gcacaatatc ccaatcgttt tttacatcac
     4081 aaactaaagt aggctgcaca aaggagccag cttgttttaa tatttctcca ccataaacaa
     4141 tttgacctcc tgccgcttta attctattta ttgctttttt aaactgttca acagcttgct
     4201 ggtcaatcaa aggacccatt aaatttcgtg tatcgagtgg gtcaccaata gttatctgct
     4261 cataagcatg tcttagcctt ttgataacat cctggtattt tgattcatga acaaacaggc
     4321 gtctcgtagt agtacaacgt tggccagcag tacccacagc accgaatact atggcaggaa
     4381 tagccaggtt caaatcagca gattcatcga ggatgatgcc gttattacct cccaactcca
     4441 gaatgctttt acccagtctg gcagcgactt ttgctgctac ttgctttcca actgcagtag
     4501 agcctgtaaa ggatattaac tgtattcgtt tatcatcaac catggcttca accacatcgt
     4561 gagaattggg aatgatcaag ccaaatattt caggacaatt attttctttt aatacctgat
     4621 tgcaaatatg ttgtactgcc actgcacaga gaggtgtttt tgccgatggc ttccatatag
     4681 taacgttgcc acaaatagca gataaaaatg cattccatga ccatacagca acaggaaagt
     4741 taaatgctga aatcacccca acaattccgt acggatgcca ttgctcatac attcggtgat
     4801 ttgggcgttc tgagtgcatg gaattaccat acaacatacg cgattgccct acagcaaaat
     4861 cagcgatatc aatcatttcc tgaacttcgc catcaccttc ctgtttggat ttacccattt
     4921 ccagcgatac cagacttccc aagctatctt tattttctct tagtgcttgc cctatttgcc
     4981 tgatgatttc accccgcttt ggagctggca cttttcgcca ggtttgcgct gcttgctccg
     5041 ctcgctgcat aacctgctca taatcatcca tagtgcaagt tgctatttca gcaagtttat
     5101 tcccagtact gggattgaag gattccagag tatgggcatg gttgtcacta tgccaaccat
     5161 gtccactaaa ggctccgggg tttactgatt taatatttaa acgttgcaat aaatccataa
     5221 gattctccta ggcataatat ttgccgaagc gattttccag cacgtgggac aatttaaatt
     5281 tttcttgcaa aataagccct ttatattcat ttccctgtcc caataccaaa tcaacaacag
     5341 cgcatacgcc cgaagctgta ctgacttgaa tagctgacca ctccaaacct cggattactt
     5401 cagggtaaat tttcttgaca taactttttt cggttagttc gccctgctta ataccctcaa
     5461 cagtaacata aactattacg atatcctgat atgtttttgg aattgcattt tccagtattc
     5521 tttttaacgt ccctcgatct tcatttaatc gtaaatcatt cattaaaaag cgcatttttt
     5581 tacaatgacc tggataacgc atggttttgt aattcatcga ttgtattttt ccagcataaa
     5641 gttcagccaa acttcccaaa cccccggacg tgttgaacgc ttcatattca caaccatcaa
     5701 tctgaattga ctccaatcct tccagtggag ccataaccac atctttacca ccttcaatac
     5761 catagcaagg attaccatat tcattaataa ccccatcagt agaccaggtt aatgaataat
     5821 gaagcgcatt attagcacgc tgtggcaatg ctccaactct tagtctggca tgatgacatt
     5881 tctcaaattc ctgcatcaaa ctattagccg caatactgat aaaaccagga gctaatccac
     5941 attgaggaac aaaagctgtc tcggcatttt gagcaatagc ttttactgcc tccgttacag
     6001 aagtatcttc cgttaaatca aaataatgag ctttggcagc tttggcggct ttggcaacat
     6061 gagtatttaa aaaataggga agactcgaaa tcactgcgat aatattgtgc ttttgcaaat
     6121 aggcctgagt ggattgttca tctttaacat ccaatgcaac cgttttaatc tcaggcagcg
     6181 cagttaataa tcttgtcaca tcagagccat taaattccag atcggccaaa tgaacctgat
     6241 aagacccact atcggcgagt agacaagcaa ttagactgcc tattttccca gcaccagtaa
     6301 tcattacgtt gtacatacaa tccttctcct ttgtaaaact tgataatcct acacaaatta
     6361 tcagagatta gatagatcca gaacacgatc aatacagcaa ttcttgatat cacttgacca
     6421 attagtctta ctcatttatc ttgaccactt taatcagtct agttgtgaca tgaagcaggc
     6481 cgctttgata cattcgacaa taataaacac atcaacacta aaccaatatt cgaagttcgt
     6541 tgttgtattt ttagcaggtt tattagcccc cttgggattt gcacctttcc atctgccagg
     6601 attaactatt ttgagtctgg ccattttatt ttctgaattt cttcaatgca cggttaagca
     6661 gggatttacc cttggtttcc tatacgggtt aggatacttt ggcttcggag tatcatgggt
     6721 gattatcagc atccacgatt atggacaact tgattacttt cttgctggcg ttatcacctt
     6781 attgtttatc atgtatcttt ctctgtttcc tggaatagtc ggttattcct ttaaactcct
     6841 ttcaaccaag tgtaatttat taacatgcat gcttttgttt agcactcttt ggtgtatagg
     6901 tgagctcatc cgttccaagc tttttactgg attcccctgg ctattaacag gtataaccca
     6961 aatcgataca ccattgagat atattgcacc agtagttggt gtttatgggc ttggattaat
     7021 atgttctttt ttgggtaccc tacttgttct gataattaaa gacaatacga ttaaacgata
     7081 ttactacctt agcgttttta ccttgattct tattgcccca tccctgggga aaaatatcca
     7141 ttggacagaa atcaagagag aacctatttc tgttggtgca atccagccta atctttcaat
     7201 gagagacaag tgggacgaaa ctctcttttg ggatttatta aaatactatg aaaacaaaac
     7261 agataaactg ttaggaaaaa atttaattgt tttacccgaa tcggccatac ccctaccagc
     7321 aagttatgtt gaagattacc tacagaaact gcatgaaaaa gcattaaaag ccaaaagcag
     7381 tttaattttg ggaatcttgc aacctacaga taatgatgaa accaattttt acaactctat
     7441 cattagcttg ggggaagcta caggagccta tgtgaaacgc caattggtac catttggaga
     7501 atatatacca aaacccttta tccccatcaa tcgttggtta aatcttccag agcctaatgt
     7561 tgttcccggc agatcgaatc agccattaat gaacatcgct aatcatccca ttgccagtct
     7621 aatttgctat gaaattgctt acccaaatat cttgagatca caaatgcctg cagctcaatg
     7681 gatagtctct atcagtgata atggctggtt tggccattcc ctggcaagct accagcaatt
     7741 acaaatggct caaatgctct ctttgcttac aggacgatac caaatcctgg tgaataatga
     7801 tggactctcc tcagtaatta accaccaagg aattattgtc gaagggctac cagcttttaa
     7861 ttcaggaata ctccaaacag atatttttcc tgcaaatggc accactccct ggataatttg
     7921 gagtgactat cctgcttttg ttttttgttt catatttgtt ctttttgttt tgttttttaa
     7981 tattaaacga tttacagaaa aataatctat tgctggcaaa ggcaagaggg gttatcctta
     8041 gcagttccgt gttattaaat ttattggcct atggataata cctataaccc ccaagaagtt
     8101 gaagaacaag cacaacaata ttggcataag aagcaatcat ttaatgttac tgaagattta
     8161 aacaaagaaa agttttattg cttatccatg tttccctatc ccagtggcac attgcatatg
     8221 gggcatgtga gaaactatac tctcggtgat gtcatagccc gatatcagcg ggcattagga
     8281 aaaaatgtcc tgcaacctat aggatgggat gcttttggcc taccagccga aaatgcagcc
     8341 atcaagaata aaattcctcc tgcagaatgg accaggaaaa acattgctgc catgaaagag
     8401 cagtttttac gcttgggaaa tgcctatgac tggaaaagag aaattactac ctgtgatcct
     8461 gaatattatc gctgggagca atggtttttt attcgtttat ttgaaaaggg cttagtttac
     8521 aagaaaaatg ctgttgttaa ctgggatcct gttgaccaaa cagtattagc taatgaacag
     8581 gtagttgacg gtcgtggctg gcgttcaggt gctttggttg agcgcaagga aatttctcag
     8641 tggtttatta aaattacctc ctatgctgat gagcttttaa gctctttaga tagcctggat
     8701 gaatggcctg cacaagtaaa acaaatgcag cgcaattgga ttggcaaatc cataggcaca
     8761 gaaatttact tcaatgtaaa caattatccc aaacgattga aaatttacac tactcgccca
     8821 gatacattga tgggagcaac ctatcttgct gtggcgacag atcatccatt agcaaaagaa
     8881 gcggcttcca acaataaaaa ggttcaagaa ttcctcgata gttgtcaagg aattaagata
     8941 gctgaagcag agctggcaac tatggaaaaa cgagggattg acactggaat gactgcaatc
     9001 caccctataa cgggtaagga gttacccata tgggttgcta attttgtttt aatgcaatat
     9061 ggttctggtg cagtgatggc agttcctgca catgatcagc gcgattggga gtttgcgcaa
     9121 aaataccaac tacctgtcaa acaggtaatc aagcctatag gcattgagca tgactttaat
     9181 caatcagctt atactgaaga aggaattctg attaattcaa atcagtttga taatctactc
     9241 tctagtaaag ccattcaagt catcacaaac tttctggaag aaaatgatgc tggcaaagct
     9301 accattaatt accgtttaag agattggggc gtttccaggc aaagatactg gggaacccct
     9361 atccccatga tcatctgtga acaatgcggt attgttccag tccctgacga agaattacct
     9421 gttgttctgc cagagaatgt tgattttacc ggtaccggtt ctccacttac acaatgcaaa
     9481 gagtttgtta atgtaacatg tccaaaatgc ggtcaagatg caactcgaga aacagataca
     9541 tttgatacct ttgtcgaatc ttcatggtac tatgctcgct ttgcttgcaa aggtcaggaa
     9601 aatgccatgc ttgatgaccg agctaaatat tggacaccag tagaccaata catcggtgga
     9661 attgaacatg cagtgatgca tctgctatat gctcgatttt tccataaatt gatgcgcgat
     9721 gaaggattag ttaattcgga tgaacccttt aaggctttat taactcaagg tatggtttta
     9781 aaagacgggc ataaaatgtc aaaatcatta ggtaatgtgg ttgatccaaa ccatcttatc
     9841 aatacctacg gagctgatac cgctcgctta tttgtcatgt ttgcatcccc tcccgagcaa
     9901 tcactggaat ggtcagattc tggagtcgaa ggagcacacc gctttttgaa acgagtttgg
     9961 gcattttcac atcagcatag agacatgctc attgacatta acgatagtat tttgagtgga
    10021 aatggtcatg tcgactggaa ggaagcagag tctcgattaa aaaaatcccg tcatattgtg
    10081 catcaaatac ttgctcaggc aacccatgac tatgaccgaa atcaatttaa cacggttgtt
    10141 tctggctgta tgaaattatt taatgaaata tctgattact ccattgaaac tgaaaatgat
    10201 aaatttttta tacactcaag catcagtata ttgctgagac tactcgcgcc aattaccccg
    10261 catatttgtc attgcctttg gcagcaatta ggttttgata aagcaataat tgatgcgccg
    10321 tggccaaagg tggataaaag tgctctaaaa actgatgaag tagattatgt tgtacaggtt
    10381 aatggaaagc tcagagctca atttactgca agcactgatg cgactgaaga ggaattaatt
    10441 gctgcagcta aggagcatgc ccacaacttt gtggtaaacc acaccattaa aaaagcaatt
    10501 attgtcccac ataggcaact aatcaacttg gtcattggat aaacatgtgt gattcgtccc
    10561 aacaatctct aaatatttta cacaatttct caagttatgg gtggcattac ctgtaagtgc
    10621 aaataagaga gggaaaagaa gaatgactca gatccacaag tacttagcat ctcttatact
    10681 gatttggctg ctaactggtt gtggttttca tttgcgtgga gcaattgata tccctgagtg
    10741 gcttgataat gtttctatta tttccaggga tggaaataaa gaactggttt ctatactcaa
    10801 atcccagctg gaaggctata aaattgatgt caattctgac ccatctctcg cgaaatactg
    10861 gttaattatt aatagttcca gtttacagca gcaaattgtt agcattggtg caagtaccaa
    10921 tccaagacaa tatcaattaa tcttaaccat tgaatttatg ttgcagactc ccaaagggca
    10981 aattattaaa acccccagaa aagttaatgt gagtcgtcaa ctcaccgtga ataatgacag
    11041 gatactaggg tcaaatgaag aacaagctct tttagtcagc gaaatgaggc gtgatgccgc
    11101 tattcaaatc attaatcgtt taaaccgacc atgatatgca aataaatcaa caagctctta
    11161 ctcagctttt acaaaaaaaa atacatcatc ttaatgtact aattggacaa gatcattatc
    11221 ttattgacga agcactcaat aaaataaaat caatgcttaa aaaatcatct gaatgtgatg
    11281 agaaaatcct atctattcag tcccctgaag aatggcatgt cgttctggaa gaaacaaata
    11341 attactctct tttttatcaa acagttattt taaccatttt ctttgataaa aaaacgatag
    11401 atacaatggg gaaaaaaatt cttggtgaat atctcaaatc agccaactct catagttata
    11461 ttattatcag agcccctaac cttaccgcca agcaacttca atggttaaca catgatgaac
    11521 aagcgctgat tatcattgca caaccattaa gctcagaagc aatgaagaga tggattaatg
    11581 agcaattaaa attaaatact atctctttcg atccatatgt tcctgaatta atttatcaat
    11641 atactaaagg aaatatgcta gctaccgctc aggtaattga aaaaattaat ttgagcaata
    11701 cacccagcac tctcattact ccttacctgg ccttggaaca tttatcggat caatgtgatt
    11761 tttccctttt tgaactgatt gattcatgcc tacttggtca tagcgacaga gcaattcata
    11821 ttttaagaca agctgccaat aataaaacag aagtcacatt ggtattatgg atgcttaccc
    11881 aagaaataag aacgttatta caattattat ttctaatgca acaaaagatg gatatcaaaa
    11941 tggcatctaa tcaattaaaa atttggactc aaagagttaa tctttatcaa tccgctgtta
    12001 aaagattaaa cactttactt ttacaacaac ttcatcacta ttgccaatct attgatgata
    12061 aaatcaaatc caactcaaac aatcaagtat ggaatgctct agagaacatt tctctttcct
    12121 tatgtttagg gcaattacta ggtgatgcat gcatagcata gcaatatttg gtggaacatt
    12181 tgacccagtg cataatggtc atatcaagac cagcttagca attcaagcta actttggctt
    12241 tgattcttat tattttttac cttgtaaaag ccccgcaatc aaacctccgt cctttgccag
    12301 cagtgagcaa agagtagaaa tgctaaagtt agcactcaaa ccttaccctg attttaaaat
    12361 agacactcga gaactggatc gagatacccc ttcttatatg gtatacactt tgcaaagttt
    12421 caggcaggag tataccgact catcattaac tctaattatt ggatacgatg gattattaaa
    12481 tctaccacaa tggtaccaat gggaaaaaat aatatcctta gccaatttac ttgttatcaa
    12541 tagagaagag ttcttccagc agcctgtgcc aaaatcagtg caaactctac ttaaccaata
    12601 tagaaatgat gataaaaata tactgctaaa tcatcatgct ggtagtatct gcctatacaa
    12661 tgctggacac tatgatattt cctcaacaaa aattagagaa caattaaaac aacataaaga
    12721 tgtaaagaat aatttgcctg atttggtata cgattacatt aagaagcagg aactgtatca
    12781 ataatatttt ctgttttatc aacagtcttg acctgcttta taaattcagg caaatggcta
    12841 tatagcaaag ctacaacagg atcaaatcgt gcataaagtt tggacttttg tgaatattcc
    12901 tgataaggca aagaggtcat tttaacagca accataatca gggcaacgat aaaaacgcca
    12961 cgtaaaaagc caaaacccat tcctaaagtt cgatcagttc cgcttaaacc agcccgtttt
    13021 aagataaagc tgagtattgc attcactacc cccccaacaa aaagggtggc aaaaagaata
    13081 ataataaatc caatagccgt tcgtactgac tgttcctcga tataagaact taaccaggga
    13141 tccaggcttt gtgaataatt aaaacctagc caaatagcta atatccacac acataatgca
    13201 acaagttcct ttacaaaacc acgaaacaat ccggtcaata cggacaaagc gataattgca
    13261 acaattgcta tatcaaccca ttgtagttgc ataactaact aactccagta ttcacaacaa
    13321 aaccatttaa ttgcatggca ctagctaatt gagtttttaa ttttattaca tcttttttaa
    13381 cgggtgaatg gccaacaact actttataaa ctattccatt tctgctatta gacttaagat
    13441 aattcgcctt gtagcctttg ctgcgtaatc gattaactaa agcttgagca ttagctattt
    13501 gtgtaaatga agccagctga acagcataaa tatcttgttt aactactggt tttctcgcag
    13561 caacaatttg gctgaccttt ttgttagtat taacctttgc cgtaacaatt ggagatgttt
    13621 tcaccggtgg ttttgctgca gccagggtca ttactttctt tgctgtcgca ttcgctgctt
    13681 tatttacagc caatttaatt ggctctgatt taattgagtt ctctctttcc gcttcaagtg
    13741 tggcgacttt agacgttctg atgagattgt cttcttctgt gctgatgggt tctgcttttg
    13801 ccaggttaat tgattttttt tcaactggta ttgggggtat ttctgtttta gccactttta
    13861 tagttttaaa tacctctttt tcatcagtaa tcactacgtc aggaggtaat ggttttggtg
    13921 gtaatttcac attgacacta aagttacttt cagggctttg gctggatttt tttattagcg
    13981 caggagcaaa aattgccccc aatgaaataa taacggccaa acctatcaag cgatgtttaa
    14041 ctttctcatc cattatcagt ttcattgatt ctccttctgc tccaaaagat tatgttgggc
    14101 agccattaca tgactaaccg taaaaaatga accataaaca ataattaaat cgtcatctga
    14161 tgcttgcttt aatgccattt caaaagctgt aaatgggtca ttataacaaa catctagaaa
    14221 aatttctgct tccctgaata cagaaagcaa ttcctcttga cttgcagcgc gcttattatg
    14281 taattgtgca ggataccatc gattaacaca atcccttaaa ggcataatca atccataaag
    14341 atctttatcc tttaaagcgg agaaaacagc atgaatttgc ccttttacat tgattttttt
    14401 tactgtatca gcaagtaatc gaactgactg cggattatga gatacatcat acaaaatatc
    14461 agtctttcct ttttctaatt gcaaccgccc cgggatataa atgagctcca tcgctttctt
    14521 tagattctta taggatacag gcaaatcatt ttgcatcaaa tgacaagcca ttacagctgc
    14581 tgccgcagaa cgaagttgaa tttttggcct ggtcaattgc tcaaatggct taccttgaca
    14641 agtaaccccc caatggtctg cttcttcctg ataaaaatag tctttaccgt ataaataaga
    14701 tgatgctcca agttgtaccg caaactcaac gacacttttt ggtggtgaga gatccgcata
    14761 gataaaaggt ttattttttc gtagtattcc ggctttctca taccctatca actcaagtgt
    14821 gtcaccaaga aaattctgat gatcaaagtc gatagtagta atgatcgcta aatcagtatc
    14881 aacaatgttt gtagcatcca accgtcctcc caagcctact tcaagaatta tcagatccag
    14941 gtcatgctgt ttgaaatacc ataaggcagc aagtgttgcc atttcaaaat aggttaaatt
    15001 ggtagggtaa cgcgcttctt caatcacaga aaaagcctga cataaatctt tatcttgaat
    15061 cggtgctaaa ttaattcgaa tacgttcatt aaacgcaaga agatggggcg acgtataggt
    15121 accaactcta aaacctgact ggtgatagat ggtttcaagt gctgtgactg tagaaccttt
    15181 accgtttgtt cctgcaacgg ttattacctt gcaatttggt cgctgcaaat ttaatttctg
    15241 cgctacagtc aaaactctgg tcaaaccaag ctgtatttcc cgggtatttc tggtttccaa
    15301 atgagtcaac cagtgactta aatcccattc actccatggc gtgtgcaaaa aaactctccg
    15361 cgcctttatg agtcaatttg gaaaccaatt cggatatagt tgctcgaaga tgtttacgtt
    15421 caactatcat atcaatatgc ccatgttcca gtaaaaactc acttcgttga aagccttctg
    15481 gcaaagtttg tctaaccgtt tgctcaatca ctcttggccc agcaaaacca attaaagcat
    15541 tgggttcagc aatgattata tcaccaagac tgccaaaact agctgatact ccacccatcg
    15601 ttggatcggt taacacaaca ataaaaggca acccggtttc agcaaatttt gccaaagctg
    15661 ctgatgtttt agccatttgc attaaagaaa ataatccctc ctgcatacga gcaccaccgc
    15721 tggcagtaaa acaaatgtat ggccgctttt ctgttgcagc aacattcact gcccttacaa
    15781 atttttctcc aaccgtcgcg cccatggagc cgcccataaa attaaactca aaggcactga
    15841 caaccactgg ttgttgcatt aaaaaccctt taacaacaat taatgcctct ttctctcccg
    15901 tcgctttttg cgcttgagaa attctatcct tgtatttttt tgagtccctg aattttaatc
    15961 tgtcctgtgg ctccagccca gcggctactt cttcttgccc tgactcatct agaaattgag
    16021 cgattctcaa gcgtgcggaa atgcgatgat gaaaattaca agatgggcaa accataaaat
    16081 tttttgccag ttcggtactg taaagcactt cattacaacc agaacattta acccataaac
    16141 cttccggaac ccccttcttt tgagaggttt ctgtccttat tcttgatggt aatagttttt
    16201 taagccagct cataaattag aacccagata ttatcaaatc ggattattat a
//
LOCUS       DBNCWK010000063        16114 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.063, whole genome shotgun sequence.
ACCESSION   DBNCWK010000063 DBNCWK010000000
VERSION     DBNCWK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16114
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.063"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            28..909
                     /locus_tag="AC06IO_001749"
     CDS             28..909
                     /locus_tag="AC06IO_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIO9874928.1"
                     /translation="MITPAELPINHRGAVYHLDLRTEEIADLIITVGDPGRVSLVSQH
                     FDKIEVQRSHREFVTHTGYLGTNRITVLSTGIGMPNIDIVMNELDALANIDLSTRTVN
                     TNKKSLTIIRLGTTGGVTANCLPGDIAISRYAIGFDSLIDYYQYQPMGVLETIREELS
                     QYLKGESGPFYISESCPQLAEQFSALGHMGITATCGGFYGPQGRQLRLPLHYPNLISQ
                     LGKFRTQSYSVINLEMETAAIYALGSMLGHKCLSLSMVINNRVHGSFVTDVKSCLDSL
                     ITKSLANIKNILEPYEA"
     gene            993..3584
                     /gene="gyrA"
                     /locus_tag="AC06IO_001750"
     CDS             993..3584
                     /gene="gyrA"
                     /locus_tag="AC06IO_001750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIO9874929.1"
                     /translation="MVYLAKEVLPVNIEDELKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMSELGNDWNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYML
                     IDGQGNFGSVDGDAPAAMRYTEVRMSKVAHALLADLDKETVDFSPNYDETEFAPVVLP
                     SRIPNLLVNGSSGIAVGMATNIPPHNLTEVINACIALVDEPDTSLEDLMEIIPGPDFP
                     TAAIINGRAGIIEGYRTGKGRVVIRARTEIETDESSGRQSIIIQELPYQVNKARLIER
                     IAELVRDKKIEGISGLRDESDKQGMRVVIELKRNEVADVVLNNLFAHTQMQNVFGINM
                     VALVDGQPRTLNLKQILEYFIKHRREVVTRRTIFELKKARSRAHLLEGLGIALANIDE
                     MIALIKQSPTPQDAKSALLSKIWQPGLVKAMLEKAGSNASRPDDLTEEYGLHENGYKL
                     SEAQAQAILELRLHRLTALEQDKIINEFEELLNLIKELLDILASPERLMQVIRDELIE
                     IKSQFGDERRTEITASQEDLTIEDLITEEDVVVTLSHQGYVKYQPITAYQAQRRGGKG
                     KSATHVKDEDFVERLVIASTHDTLLCFSNHGKLYWLKAYQLPQASRASRGRPIINILP
                     LAEGEEINAMLPVREYKDGSYVFMATKKGTVKKVPLNAFSRPRSNGIIAVDLEEDDSL
                     VGVDITDGARDIMLFTDAGKVIRFDESKVRPMGRTARGVRGIRVEKDQAVKSLVVVDP
                     SGGTILTATENGYGKRTHIDEYRVSGRGGQGVISIQVTERNGKVVRSLQVTDNDEAML
                     ITDKGTLVRFKVNELSVIGRNTQGVRLINVSSGEAVVGMQKIVDLGEELEEAEDSSLN
                     AEDSSDE"
     gene            3577..4665
                     /gene="serC"
                     /locus_tag="AC06IO_001751"
     CDS             3577..4665
                     /gene="serC"
                     /locus_tag="AC06IO_001751"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIO9874930.1"
                     /translation="MNSRIFNFGAGPAMLPEEILKEAQEEFLNWRNTGMSILEIGHRT
                     PEIISLLSTAEQSLRELLNIPKNYHVLFLGGAARTQFAMIPMNLLRPGDDAAYFITGI
                     WSKMAYHEANLLKKAYYLSSEEKEGFVSIPDYQKWELKSNTAYVYYTPNETINGVRFP
                     YVPKTEGVPLVADMTSCLLSEPINIRQYGLIFAGAQKNIANAGLTVVIIHEELLQNQP
                     EPVIPTMLNYKNHAEHRSLYATPPVFNCYLASKMFEWIKTQGGIEGLFQRNCLKAAKL
                     YQYLDSTDFYLTPVSKEARSIMNVCFSLYYPDLEQKFLDMANERGLKALKGHRFTGGL
                     RASLYNAMPMAGVDALIEFMSEFAKENG"
     gene            4671..5972
                     /gene="aroA"
                     /locus_tag="AC06IO_001752"
     CDS             4671..5972
                     /gene="aroA"
                     /locus_tag="AC06IO_001752"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIO9874931.1"
                     /translation="MLNFISKPVGCLKGEITVPGDKSISHRSIIFGAIAIGTSVIDGF
                     LDGEDCIATLKAFQSMGVRIEGPDKQRVIIHGVGKYGLKQPQNIIDCGNSGTSMRLLA
                     GLLAAQQFDSQLTGDESLLKRPMLRISRPLSQMGADVTTQDGKPPIVIKGGKKLNGIH
                     YVMPEASAQVKSCLLLAGMYAEGQTKITENAVSRDHTERMLRTFSYPVQIQDGTIVID
                     RNGECYGTRLNIPGDISSAAFFIVAASITPDSDVLIRNVGINPTRTGIIHILTEMGAD
                     IRILNQRAYGEEPVADLHIRYSQLKGIDIPVSMVPLAIDEFPVIFIAAACAQGKTTLH
                     GAKELRLKESDRIGAMVDGLNQLGVHAEGFDDGILIEGGSIQGGEVNSRGDHRIAMSF
                     AIAGAVASAPVTIKNCANVATSFPSFVTTANMLHFQIEEYS"
     gene            5972..6664
                     /gene="cmk"
                     /locus_tag="AC06IO_001753"
     CDS             5972..6664
                     /gene="cmk"
                     /locus_tag="AC06IO_001753"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIO9874932.1"
                     /translation="MVFNNEVPVITLDGPSGTGKGTICHLVAKRLHWNMLDSGAIYRV
                     LAYAARKNSIEPNDIKKLTALARSLNLRFESSADNESKVILDDENVSQQIRSEQCGQD
                     ASQIAVIPEVRAALLERQRNFAQFPGLVTDGRDMGTVVFPNAVLKVYLYASAEERANR
                     RYLQLQDNGINVSLAQVVEELAKRDARDTARTHAPLKPAEDAVLIDTTGLTIVQVFNI
                     VLKLIDERLNNL"
     gene            6730..8406
                     /gene="rpsA"
                     /locus_tag="AC06IO_001754"
     CDS             6730..8406
                     /gene="rpsA"
                     /locus_tag="AC06IO_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIO9874933.1"
                     /translation="MSESFKELFEQSIAGAQFYPGAIITAKVIDIDDDFVTLNAGLKS
                     EGIVAVEEFYDKNGELEVKVGDTVEVALDSVEDGHGETLLSREKAKRQEAWRKLSKCH
                     ENNETVTGLISGKVKGGFTVEIGSIRAFLPGSLVDVRPVRDPSYLEGKELEFKVIKMD
                     LKRNNIVVSRRAVVEEESSADRQALLESLHDGQELHGIVKNLTDYGAFIDLGGIDGLL
                     HITDISWKRVKHPSEVLSVGQDVKVKVLSFDSERNRVSLGMKQLGNDPWVDLVERYPI
                     GKRLQGKVTNITDYGCFVEIEEGVEGLVHMSEMDWTNKNVHPSKVVSLGDVVDVMVLE
                     IDEERRRISLGMKQCVGNPWQQFASTHNKGEKVKGKIRSITDFGIFIGLDGDIDGLVH
                     LSDISWTVPGEEAVKQFKKGQELEAVILAIDPERERISLGLKQLEGDSFASFAETYTK
                     GAIVKGTVTAVEPKTVTVALAEDVSGTIRVSELSDERVDDATTIVKVGDEVEAKITNI
                     DRKNRTISLSVKAKDAQDEADAIKKYSRTEAASTTLGDLLKEKMASKEGE"
     gene            8520..8810
                     /locus_tag="AC06IO_001755"
     CDS             8520..8810
                     /locus_tag="AC06IO_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIO9874934.1"
                     /translation="MRILMLVTYLLLIIIGVSFAALNASSVQVNFYFKTVSMPISVLM
                     TIMLGVGVFIGFVLFITRYWRLKIECRKMKNQLKLTEKEIKNLRSIPLQDQH"
     gene            8857..10026
                     /gene="lapB"
                     /locus_tag="AC06IO_001756"
     CDS             8857..10026
                     /gene="lapB"
                     /locus_tag="AC06IO_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIO9874935.1"
                     /translation="MINLWPLLLPAAAWSGWWLANRSNSNKDAHVHNRLSREYVVGLN
                     YLLNEQSDKAVDVFIKLLEVDSDTVETHLALGSLFRRRGEVDRAIRIHQNLIARPQLS
                     IQQRKEALMALGQDYMSAGVFDRAERIFLEVVELGGSRETSSLQGLLAIYQQEKAWEK
                     ALDIIKKLEISTGASFHNQAAHYYCEMASQALKINAMDRAIYCIKQAMNVDPESVRAS
                     LMNATIEIKEGRYKQAIRSLKRVPQQDAEFLTEIIEPLVLCHKELDSMQDCIHYLEQT
                     LEKHPRASVIFVIADYLRQNKGMDIAIDFVADNLSKHPSIRGLNRLIYWHLESAHGKV
                     REKLQMLYDITSKFLDNKPIYRCGHCGFGGKHLHWHCPSCKQWGRMKPIHGLEGD"
     gene            10280..11395
                     /locus_tag="AC06IO_001757"
     CDS             10280..11395
                     /locus_tag="AC06IO_001757"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIO9874936.1"
                     /translation="MQFIDLKTQYSLIENDILNSIKRVLNHGQYIMGPEIAELEKELA
                     TFVGVKHAIVNSSGTDALLMALMALDIQPGDEVITSPFSFFATAEVISLCHATPVFVD
                     IDPLTYNLDPEQLERAITKKTKAIMPVDLYGQCADYDAINAIAAQYGIPVIEDAAQSF
                     GATYKGKHSCSLATIGCTSFFPSKPLGGYGDSGACFTNDDVLAQKLIEIRIHGQNARY
                     CHHRVGINGRMDTIQAAILLQKLKIFSNEILMRQKVAKRYDQMLSELVKTPYVHECNA
                     SVYAQYTIEVENRDVFAKSMQASGIPTAVHYPIAMHQQQALGYLNYKLGDFPNSEKAS
                     QHVISLPMHPYLQEDEQLKIVAAVRNALLVMNEEAMV"
     gene            11396..12085
                     /gene="pyrF"
                     /locus_tag="AC06IO_001758"
     CDS             11396..12085
                     /gene="pyrF"
                     /locus_tag="AC06IO_001758"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIO9874937.1"
                     /translation="MTPKLIVALDFDNQDNALQLVEKLDPNHCALKVGSELFTLLGPQ
                     FVKELARREFKVFLDLKFHDIPNTVAKACHSAAELGVWMINVHAIGGLKMLQAARESL
                     KTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
                     VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNP
                     YEVVSALLRDL"
     gene            12272..14926
                     /gene="vpdC"
                     /locus_tag="AC06IO_001759"
     CDS             12272..14926
                     /gene="vpdC"
                     /locus_tag="AC06IO_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VpdC"
                     /protein_id="HIO9874938.1"
                     /translation="MMTPQHVISQLLESDTKYPQNLDLLKKIIISAYLGRLRINGLPP
                     DNKIALGNYLFDDERMIFDFTRLSDSKRALFIEWLLEPHQKDKEHGFLSGVYVNEYRG
                     FTAEVALSWWGILKNWLFNNKSEHWKITDLGLSVNYQLTGIEMCHGKQGILIGFNQFL
                     VPPSGTKYRDPNDSQSEPLGNVKRVFITDKLVDELVGLNYKNLNYESVCKSPHPQSID
                     VTDPEVRHKDMYDYRKMQRFNGVRPWYIRLWRWISKLFFGDTYDAKKTSTSDDNLELL
                     YKTKTVKIYQRSKSKEILVTEKRPDITNLVFCGGGAKIFAHVGVWKALNEAGMKPTKF
                     AGSSAGAIMSLMCYLGYTADEISELFKHFKQEHLVQFDIDRNGLSDTHSLKTALDYAI
                     ANKVRQIVNKYKIPYPKGKITFSTLASLREQFPDCGIGKELIVTATNKRSGKTIYLSA
                     NRYPLLEVSEAVKISASFPVLYRDTLLDGEEHNDGGILSNFPTEVFFDDHSTLLESEF
                     GNNLKVLAVQFDNGIERNVIDRIMDKVYRENFVLNWIYSLLTGVSDPASGWERDRLKL
                     RQYALQTIIPETGKVSTTGFNVERQSQEELIQNGYQSTLDYLNVRYTRKNNSYVNKEI
                     MYSTFNSLGDLLAYCCYRGDEQWFNVVNNLIVQSTLPNRTALMKQSLELRKLYFNTSL
                     PSNPEKNKTACTESNTLTFFGNAVCQHHNHSQELENGNHDVLLALYPIFLKLSTDLLI
                     NKIDKNLLDRARHSLNIHSPFDCLEHFNKISGNAHVMIHIIVKLIKELKERSCQQIYE
                     VLKEVRHLLYSDINLMKANYFGKWDLSLPQCIRILNLLKLEKHDDVSRLLDCLRNQME
                     PVQTVKGGVHHDDLNNGGHERYRLGLGV"
     gene            14871..15650
                     /locus_tag="AC06IO_001760"
     CDS             14871..15650
                     /locus_tag="AC06IO_001760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9874939.1"
                     /translation="MMTLITGATRGIGLALAYEFASHGHDLILIGRDSEQLEHISKQL
                     NTQYQVNVEYKVHDLSKPGSAFKLYTDLKEQGVEVNCLVNNAGIGYMGSFVEMGMNKL
                     DDLMNINMISLSELTLCFLHDFVARNEGSILQVSSTAAFQPGPFMAAYYASKAYVATL
                     SHAIAYELKDTKVSMSILCPGATKTNFFHASGMESSMLERGYIGMMTPEKVAKIAYKG
                     LKKGKLYIIPGLRNKILAYAAAISPKAIALRIASYLHHKIL"
     gene            15656..>16114
                     /locus_tag="AC06IO_001761"
     CDS             15656..>16114
                     /locus_tag="AC06IO_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HIO9874940.1"
                     /translation="MRLQLMKITGFTLLETMLALALLLGLLLLCSWSFFSLIQNNERE
                     TLVNSIKTAIQYSKIQAIHLGHPIYLLPFGSNENWSRGMVLAELNRKSNKTELIYQWQ
                     WSSNSWNINWKGVDSNHRIIISNIPNRAMSNGKFILNNRRTNEKVVVTLNR"
BASE COUNT         5085 a         2623 c         3523 g         4883 t
ORIGIN      
        1 caaatttgag gaataataga aaaaaaaatg ataactccag ctgaattacc tattaatcat
       61 cgtggtgcgg tatatcatct tgatttaaga acagaagaaa tagctgatct catcattaca
      121 gttggagacc ctgggcgagt aagcctggtc agtcaacatt ttgataaaat tgaagtacaa
      181 cggtcccatc gagagtttgt tacccatact ggttatctcg gaaccaatag aattacagta
      241 ctctccactg gcattggcat gccaaatata gatatcgtga tgaatgaact ggatgctttg
      301 gcaaacatcg atttatctac acgaacggtg aatactaaca aaaagagttt aacaataatt
      361 cgattgggca ccacaggagg ggtgactgcg aattgtctac cgggagatat agcgattagt
      421 cgatatgcca ttggttttga tagtttgatt gattattatc agtatcagcc gatgggagtg
      481 cttgaaacaa ttagggagga gttatctcag tatctaaagg gagagagtgg cccattttat
      541 atttctgaat catgcccgca attagccgaa cagttcagtg ccttaggcca tatgggaatt
      601 acagccactt gtggtggttt ttatgggccg caaggtcgcc agttaagatt gccgcttcat
      661 taccccaatc ttattagtca gttaggaaaa tttcgtactc agagttattc agtaattaat
      721 ctagagatgg aaaccgctgc tatctatgct cttggcagta tgcttggaca taaatgctta
      781 tccttaagta tggtgattaa taacagggta catggtagtt ttgtaactga tgtcaaatct
      841 tgtctggatt ccctaattac aaaatcactt gcgaatatta aaaatatcct tgagccatat
      901 gaagcttaag gtcatttaat taagacgccc agtgtgatat ccttttgcat taaattaatc
      961 ttaatttatt gtctaaagga tacatccaaa tcatggtata cctagccaaa gaagtcttac
     1021 cagtcaacat agaagacgaa ttgaagcaat cctatttgga ttatgcgatg agtgtcattg
     1081 taggccgagc attgcctgat gtacgtgatg gtttaaaacc ggtgcatagg cgagttcttt
     1141 ttgcgatgag cgagttgggt aatgattgga ataaacctta taaaaaatct gctcgtgtag
     1201 taggggatgt catcggtaaa taccatcctc atggggatac agctgtttat gacactattg
     1261 ttcgtatggc tcagcccttt tccatgcgtt atatgctgat tgatgggcag ggtaattttg
     1321 gttctgtaga cggagatgct ccagctgcca tgcgttacac tgaagtacga atgtccaagg
     1381 tggcgcatgc tttactggct gatttggata aggaaaccgt tgattttagt cctaactatg
     1441 atgaaacaga atttgctcca gtggtattgc catcgagaat tcccaattta ctagttaatg
     1501 gctcttccgg tattgcggta gggatggcta ctaatattcc accacataat cttaccgaag
     1561 taatcaatgc atgtattgcc ttagtggatg aacctgacac gagtcttgaa gatttaatgg
     1621 aaattattcc tggccctgat tttcctacag cagcaattat taatggtcgt gctggaatta
     1681 ttgaaggtta tcgtactgga aaagggcggg ttgttatcag ggcacgcaca gaaattgaaa
     1741 cggatgaaag ttcaggccgt cagtcaatta ttattcagga attaccctat caggtgaata
     1801 aagcgcgttt gatcgagcgt attgctgaat tggtaagaga caagaaaatc gaaggaattt
     1861 ccggcttgag ggatgagtca gataagcagg gaatgagggt agtcattgag ttaaaacgca
     1921 atgaagtagc agatgtggta ttgaataatc tgttcgctca tactcaaatg caaaatgtat
     1981 tcgggattaa tatggttgcc ctggtggatg gccaaccgcg tactttgaat ttgaagcaaa
     2041 tactggaata ttttataaaa catcgaagag aggttgttac cagacgcaca atatttgaat
     2101 tgaaaaaagc cagaagtcga gcccatttat tggagggctt gggaatcgcc ttggctaata
     2161 tcgatgaaat gatcgcatta attaagcaat ctcctactcc tcaagacgca aaaagtgctt
     2221 tattaagcaa aatatggcaa cctggcttgg taaaggccat gttggaaaag gctggctcaa
     2281 acgcgtcaag gcctgatgat ttaactgaag agtatggttt gcatgagaac ggatataaat
     2341 tatctgaggc gcaggcacaa gcaatacttg aattaagatt acataggcta acagctcttg
     2401 aacaagataa aataattaat gaatttgaag agctattgaa tttaattaaa gaattgcttg
     2461 acatattggc ttcacctgaa agacttatgc aagttattcg tgatgaattg atagagatta
     2521 aatcccaatt tggtgatgag aggcgaacgg aaataacagc ctcacaagaa gatttgacta
     2581 ttgaagattt gattaccgaa gaagacgttg tagtcacttt atctcatcaa ggttatgtaa
     2641 aatatcagcc cattacagcc tatcaggctc agcgccgagg tggcaaaggc aagtcggcaa
     2701 ctcatgtcaa ggatgaggat tttgttgagc gcttggttat tgcgagtact catgacacct
     2761 tattatgttt ctccaatcat ggcaaattat attggctaaa agcatatcaa ttgcctcaag
     2821 caagccgcgc ttcgagagga aggcctataa tcaatattct tcctttggct gaaggcgaag
     2881 agattaatgc tatgctccct gtccgtgagt ataaagacgg cagctatgtg tttatggcaa
     2941 ccaagaaggg aacagttaaa aaagtgcctt taaacgcatt tagcaggccg cgttccaatg
     3001 gtattattgc tgtggacctg gaagaggatg acagtctcgt tggagttgat attactgatg
     3061 gtgctcgcga tattatgttg tttactgatg caggcaaagt gatccgcttt gatgaaagta
     3121 aagtgcgacc tatggggcgc acggctcgag gagttcgtgg aattagggta gaaaaggatc
     3181 aagccgttaa atctcttgtg gtagtcgatc caagtggagg caccattttg acggcaactg
     3241 aaaacggcta cggcaagaga actcatattg atgaatatcg agtgtcagga cggggcgggc
     3301 aaggtgttat ttccattcag gttactgaac gaaatggtaa agtggtccgc tccttgcagg
     3361 ttaccgataa tgacgaagcc atgttaatta cagataaagg gactttggtt cgttttaaag
     3421 ttaatgaatt atctgtgata ggccgaaata cacaaggagt tcgtctgatt aacgtcagtt
     3481 ctggcgaggc agttgttgga atgcaaaaaa ttgtagatct tggagaagaa ttagaagagg
     3541 cagaagattc atcccttaat gctgaagaca gcagtgatga atagcaggat tttcaatttt
     3601 ggagctggcc ctgccatgct gcctgaggag atattaaaag aggctcaaga ggaatttctt
     3661 aattggcgta ataccggcat gtctatactt gaaatagggc atagaacacc ggaaatcatt
     3721 agtcttttaa gtaccgcaga gcaatcgtta agagagctgt taaatatccc caaaaattat
     3781 catgtattat ttttgggggg ggcagctcgg acacaatttg ccatgattcc tatgaattta
     3841 ttacggccag gagatgatgc ggcttatttt attacgggaa tctggtctaa gatggcttat
     3901 catgaagcga atcttctaaa aaaagcatat taccttagca gtgaagagaa agaaggattt
     3961 gtatcaattc ctgactatca aaagtgggag ttaaaaagta atacagccta tgtctattat
     4021 accccaaatg aaacaattaa tggagtcaga tttccttatg ttcctaaaac agaaggtgtt
     4081 cctctagttg cggatatgac ttcttgcttg ctgagcgagc cgataaatat taggcaatat
     4141 ggtttgattt ttgctggtgc gcagaagaac attgccaatg caggtttaac tgtagttatc
     4201 attcatgagg aattactaca aaatcaacct gagccagtga ttcctaccat gttgaattat
     4261 aaaaatcatg ccgaacatcg ctccttatat gctacaccac cagtatttaa ttgttatttg
     4321 gcaagtaaaa tgttcgagtg gataaaaact cagggaggta tagaaggctt atttcaaagg
     4381 aattgtttga aagctgcgaa gctgtatcaa tatctcgatt caactgattt ttatttaact
     4441 ccagtatcta aagaagctcg ctctataatg aatgtttgct tttccctgta ctatccggat
     4501 ttggaacaaa aatttctcga tatggctaat gagcgtgggt taaaggcttt aaaaggccat
     4561 cgtttcactg gaggattgcg cgccagtttg tacaatgcca tgcccatggc tggagttgat
     4621 gctctcattg agtttatgtc tgaatttgca aaagaaaatg gttaataagc atgttgaatt
     4681 ttataagtaa accagttggc tgccttaagg gcgaaatcac agtacctgga gataaatcca
     4741 tttctcatcg ttctattatt tttggtgcga ttgctattgg aacttcagta atcgatggct
     4801 ttttagatgg tgaagattgc atagccaccc ttaaagcatt ccaatcaatg ggtgttagga
     4861 tagagggtcc tgataagcaa cgtgtcatta ttcatggggt tggtaaatat ggtttaaaac
     4921 aacctcaaaa tattattgat tgtggcaact caggcaccag tatgcgcttg ctggctggat
     4981 tgcttgcagc acaacaattt gatagccagt taacgggaga cgaaagttta cttaaacgcc
     5041 caatgttaag aattagcaga cccttaagtc aaatgggcgc agacgttact actcaggatg
     5101 ggaagccgcc tatagtaatc aaaggcggaa aaaaattaaa tggaatacat tatgttatgc
     5161 cggaagccag cgctcaggta aagtcgtgtt tgttgttagc tggtatgtat gctgaaggac
     5221 aaacaaaaat aactgaaaat gcagtcagtc gggatcatac tgaaagaatg ttaagaacat
     5281 tttcttatcc agttcagata caagatggta ctatcgtaat tgatcgtaat ggtgagtgct
     5341 atggaactcg tctgaatatc ccgggagaca tttcatctgc tgcatttttt attgttgcag
     5401 cttctattac tccagactcc gatgttttaa ttcgtaatgt aggcattaat cctacacgaa
     5461 ctggcattat tcatatttta actgaaatgg gagcggacat aagaattttg aatcaacgtg
     5521 cctatggaga agaaccagtt gctgatttgc atatcagata ttcccaatta aaaggaatcg
     5581 atattcctgt ctctatggtg cctttggcga ttgatgaatt cccggttatt tttattgcag
     5641 ctgcttgcgc acaagggaaa accactcttc atggagctaa agagctgcgc ttgaaggaaa
     5701 gtgatcgtat tggcgcgatg gtggatggct taaatcaatt gggagttcat gctgagggtt
     5761 ttgatgatgg aatattgata gagggtggaa gtatacaggg tggtgaggtt aatagtcgag
     5821 gagatcaccg tattgccatg tcatttgcca ttgctggcgc tgtcgccagt gctcctgtaa
     5881 cgattaaaaa ctgcgccaat gttgcaactt catttccatc ttttgttact accgctaaca
     5941 tgcttcattt tcagatagag gaatacagct gatggttttt aataatgagg ttcctgtcat
     6001 tacgttggat ggtccaagtg gaacaggaaa ggggacaatt tgtcatttgg tagccaagag
     6061 gctacattgg aatatgcttg acagtggtgc tatttatcgt gttctagctt atgctgctcg
     6121 gaaaaatagc atcgaaccga atgatattaa aaaattgact gctttggccc gttccctgaa
     6181 tttgcgtttt gaatcatctg cggataatga atcaaaagta atacttgatg atgaaaatgt
     6241 cagccagcaa atccgcagtg agcaatgtgg tcaggatgcc tcacaaattg cagtgattcc
     6301 tgaagtaaga gcagcactgc ttgaacgcca gagaaacttt gcccaatttc ctggattggt
     6361 gaccgatgga cgcgatatgg ggacggtggt ttttccaaat gctgttttga aggtttattt
     6421 atatgcaagt gcagaagaaa gagcaaatag acgatattta cagttgcaag ataatggaat
     6481 taatgttagc ctcgcccagg ttgttgaaga actggctaaa cgggatgcaa gggatacagc
     6541 tcgtacgcat gcgccattga aaccagctga agatgctgtc ttgatcgata caaccggact
     6601 aacaattgta caagtgttca atattgtatt aaaattaatt gatgaacgtt tgaataattt
     6661 gtagttttga attgggggga aggtgagtgg aattcattct tccatttttt tactaaagag
     6721 tttattaaca tgtctgaaag tttcaaagaa ttatttgagc aaagtattgc cggagctcaa
     6781 ttttatcctg gtgccattat tactgctaaa gtcatcgata ttgatgatga ttttgttact
     6841 ctcaatgctg gactaaagtc agaaggtatt gttgccgtag aagaattcta cgataaaaat
     6901 ggcgaattgg aagttaaagt aggcgatact gtcgaagtgg ccctggattc agtagaagat
     6961 ggtcacggcg aaacgctcct ttcaagagaa aaagcaaaac gccaggaagc atggcgcaag
     7021 ttatcgaaat gccacgaaaa taatgaaaca gtcactggtt tgatttctgg aaaagtcaaa
     7081 ggtggtttta ctgtggaaat tggctcaatc agagcatttt taccgggttc ccttgttgat
     7141 gttaggcctg taagagatcc atcttacctc gaaggtaaag aacttgaatt taaagtcatc
     7201 aagatggatt tgaaacgcaa taatattgtt gtttctcgac gagctgtagt tgaagaagaa
     7261 agtagcgctg acagacaagc actactggag tctttacatg atggtcaaga gcttcacggt
     7321 attgttaaaa accttaccga ctatggtgct ttcattgatc ttggcggcat agatggtttg
     7381 ttgcatatca ctgacatttc ctggaagcga gtcaagcatc caagtgaagt attatcagta
     7441 ggtcaggatg ttaaagtcaa agtactaagc tttgatagtg aaagaaaccg cgtttccttg
     7501 ggtatgaagc agttaggaaa tgatccttgg gttgatttgg ttgaacgtta cccaataggc
     7561 aagagattgc aaggtaaggt aaccaacatc accgattatg gttgctttgt cgaaattgag
     7621 gaaggtgtag aaggtttagt ccacatgtca gaaatggatt ggactaataa aaatgttcac
     7681 ccaagcaaag tagtgtctct gggagatgta gtagacgtta tggttcttga aattgacgaa
     7741 gaacgacgac gtatttcttt aggcatgaaa cagtgtgtgg gtaatccatg gcagcaattc
     7801 gcatctactc ataataaagg cgaaaaagta aaaggcaaga tccgttctat tactgacttt
     7861 ggaatcttta taggtcttga tggtgatatt gatggactag ttcatttatc tgatatttcc
     7921 tggactgttc ctggtgaaga agcggtaaaa caatttaaaa agggtcaaga gttagaagct
     7981 gttattttag caatcgatcc agagagagag cgtatttctt taggattgaa acaattggaa
     8041 ggcgacagtt ttgccagctt tgctgaaact tacacgaaag gagctattgt taaaggtaca
     8101 gttacagctg tagaacctaa aactgtaaca gttgcattag cagaagatgt ttctggcaca
     8161 attcgtgtga gtgaattatc cgatgaacgt gttgatgatg cgaccactat cgtaaaagtt
     8221 ggtgatgaag ttgaagcaaa aatcactaat attgatagaa aaaatcgcac catttcactt
     8281 tccgtcaagg caaaagatgc ccaggatgaa gcggatgcca ttaagaagta ctccagaaca
     8341 gaggcagcat caactactct gggtgattta ttgaaagaaa aaatggctag caaagaaggt
     8401 gaataacctt tttaagtcaa aactaaaaaa gcgtagagct atctacgctt tttttttatt
     8461 ttgagaaaaa gattatggta tgatggacgt tttagcttaa ggattaaagg aaatattcaa
     8521 tgcgcatatt aatgctggta acttatttgc tgcttattat tattggtgtt agttttgcgg
     8581 ctctaaatgc ctcgtcggtt caagttaatt tctattttaa aacggtgtcc atgcctatct
     8641 ctgttcttat gactatcatg ctcggagttg gtgtttttat aggttttgtt ctctttatta
     8701 cacgatattg gcgtttaaaa attgaatgtc gtaaaatgaa aaaccaatta aaactaactg
     8761 aaaaagaaat taagaattta cgttcaatac ccttacaaga tcaacattaa ttttattatt
     8821 agtggaaact cgatatgcag agaggaaaat taatagatga ttaatttatg gccactgttg
     8881 ttacctgctg ccgcatggtc tggctggtgg ctggctaacc gcagtaattc caataaagat
     8941 gctcatgtcc acaatcgctt atcgcgagaa tatgtagttg gacttaatta tttattaaat
     9001 gaacaatctg ataaagcggt tgacgtattt atcaagcttc tagaagttga tagcgataca
     9061 gtagaaaccc atcttgcact gggtagcttg ttcaggcgta gaggggaagt agacagggct
     9121 attcgtattc atcaaaattt aatcgccagg cctcaattaa gtattcaaca acgaaaggaa
     9181 gccctaatgg ctttagggca agattatatg agtgctggag tgtttgaccg agccgaacgt
     9241 atattccttg aagttgttga gttgggcggg agtagagaaa caagcagtct tcaaggatta
     9301 ttagcaattt accaacaaga aaaagcttgg gaaaaagctt tagacattat taaaaaactg
     9361 gaaatttcta ccggagccag tttccacaat caagcagctc attattactg tgaaatggct
     9421 agtcaggcgc ttaaaatcaa tgcaatggat agagcaattt attgtataaa gcaagcaatg
     9481 aatgttgatc cggaatccgt aagagccagt ttaatgaatg ctacaattga aataaaagaa
     9541 ggccgctata aacaggctat tcgatctttg aaaagagtgc cacagcagga tgcagaattt
     9601 ttaaccgaaa tcattgaacc attagttctt tgtcataaag agctggactc catgcaggat
     9661 tgtattcact atcttgagca aactttggaa aaacatccga gagcctctgt aattttcgtt
     9721 atagctgatt atttgcgaca aaataagggt atggatattg caattgactt tgtggctgac
     9781 aatttaagta aacatccatc gataagaggt ttgaatcgtt tgatttattg gcacttggag
     9841 tctgctcacg ggaaagtacg tgaaaaactg caaatgcttt atgatattac tagcaagttt
     9901 ttagataata agcccatata tcgatgtggg cattgtggtt ttggtggtaa acatttacat
     9961 tggcattgcc caagttgtaa acagtggggt agaatgaagc ctatccatgg tttggaaggg
    10021 gattaacctg agacatactc tcagaaagtt aatgaacagt tgcatattag cttagtatcg
    10081 taattcaaat ttgttattgc aaattaagta aaacaattcg aacaggaaat ttgatttgtt
    10141 ttgtcttctc gtttgccaag gcagtttacg ttcaaatcag tttattgcaa ctttagattt
    10201 gtattaagat tattccttaa cttcatggta ttgatcttag gtatagctaa acaatttata
    10261 tactaattat gagattataa tgcaatttat cgacttaaaa acacaatatt ctttaattga
    10321 gaatgatatt ttaaacagca ttaaaagggt attaaatcat ggccaatata ttatggggcc
    10381 tgaaattgca gaacttgaaa aagagttagc cacttttgtt ggagtgaagc atgcgattgt
    10441 gaattcaagt ggtacagatg ctttattaat ggcattgatg gctctggata ttcagcctgg
    10501 ggatgaagta attacaagcc catttagttt cttcgcaaca gcagaagtga ttagcctttg
    10561 tcatgcaaca cccgtctttg tagatataga tcctttaact tataatttgg atcctgagca
    10621 gttagagaga gcaatcacta agaaaaccaa ggccattatg ccagttgatt tatatgggca
    10681 atgcgcagat tacgatgcga ttaatgccat tgcagctcaa tacggtattc ctgttattga
    10741 agatgctgcg cagagttttg gagctactta taaaggtaaa cactcttgtt ccctggctac
    10801 tattggctgt accagttttt tcccgtcaaa acctttaggt ggttatggtg attctggagc
    10861 ttgttttact aatgatgatg ttttagccca aaaattaatt gaaattagaa ttcatgggca
    10921 aaatgcccgc tattgtcatc atcgggtagg gattaatggt cgaatggaca ctatccaagc
    10981 agcaatacta cttcagaagc tgaaaatatt ttctaatgaa attctaatgc gacaaaaagt
    11041 agcaaaacgt tatgatcaaa tgctttctga gttagtgaaa actccttatg tgcatgaatg
    11101 caatgcgagt gtgtatgctc agtatactat tgaggttgag aatagagatg tatttgcgaa
    11161 gtcaatgcaa gcctcaggta tacctacagc tgttcattac ccaattgcta tgcatcaaca
    11221 gcaggcactt ggttacttaa attacaaact tggagatttt ccaaactctg aaaaagcaag
    11281 tcaacatgta attagcctgc ctatgcaccc ttatttacaa gaagatgagc aattgaaaat
    11341 agttgcagca gttagaaatg ctttacttgt gatgaatgaa gaggccatgg tataaatgac
    11401 acctaaatta atcgttgcgt tggattttga taatcaagat aacgctttac agttggttga
    11461 aaaacttgat cctaatcatt gcgcacttaa ggttgggagt gagcttttta ctctattagg
    11521 gcctcagttt gtaaaagaat tggcaagaag agagtttaaa gtatttttag atttaaaatt
    11581 tcacgatatc cctaatacag ttgctaaagc atgccactcc gctgcagaat taggggtatg
    11641 gatgataaat gttcatgcca tcggcggctt aaaaatgttg caagctgcca gggaatcttt
    11701 aaaaacgtat ggtaaagaca ggcctttgtt gattgcagtg actgtgttga ccagttttga
    11761 agaaggggag ctggccagtg ttggtattag taatacattg cctgagcaag caactcatct
    11821 ggctatgctt gccagagagg ctgggcttga tggtgtggta agctctgcac atgaagttaa
    11881 aattataaaa cagaagtgtg gtgagaattt tattaccgtt actcctggca taagactgcc
    11941 aaataatcta aaagatgatc aatcgcgagt aatgactcca caacaagcca tcagagaagg
    12001 aagtgatttt ctggttattg gaagaccaat tacgcaagcg agcaatcctt atgaggttgt
    12061 ctcagcacta ttgcgtgatc tttgatggtg catactttta aaaaattata catatggtat
    12121 aattttgttc atttttgaat tagttgtacc attcctattt tattccatag aaatttatga
    12181 taattgttat aaagatgatt aataaatatt taaaaataac ccatattatt tcagatattt
    12241 attctatgga ctacccagac gtaaggattt actgatgacc ccacagcacg tcataagtca
    12301 actgctggaa tctgatacca aataccctca gaatttagat ttattaaaaa aaataatcat
    12361 atcagcttat ctgggacgcc tcagaattaa tggattacct ccagataaca agattgcact
    12421 tggaaattat ttatttgatg atgaacgtat gatttttgat ttcactcgat tgagcgattc
    12481 aaaaagagct ttatttattg aatggttgct tgagcctcat caaaaagata aagagcatgg
    12541 ttttttaagt ggcgtttacg ttaatgaata tcgaggtttc actgcggagg ttgctttaag
    12601 ttggtggggt atcttaaaaa actggttatt taataataaa tctgagcatt ggaaaataac
    12661 ggatttggga ctttctgtca attatcagtt gactggtatt gaaatgtgtc atggaaagca
    12721 gggtatctta attggattta accagttttt agtaccacct tcaggtacaa aatacagaga
    12781 tccaaatgat agccaaagtg agccattagg taatgttaaa agagtattca ttactgataa
    12841 actggttgat gaacttgttg gactcaatta taaaaatctt aattatgaat ctgtctgtaa
    12901 aagccctcat cctcaatcta ttgatgtaac tgatccagaa gttcgtcata aagacatgta
    12961 tgactacaga aaaatgcaac gtttcaatgg tgtgaggcct tggtatatca gactttggag
    13021 atggatatcc aaactatttt ttggcgatac atacgacgct aaaaaaacaa gcacttcaga
    13081 cgacaatttg gaattacttt ataaaactaa aactgtcaaa atttatcagc gctccaaatc
    13141 aaaagagatc ttggtcacag agaagcgacc tgatattact aatttggttt tttgtggagg
    13201 tggggcaaaa atttttgctc acgtgggggt ttggaaggct ctcaatgaag caggaatgaa
    13261 gccaaccaaa tttgcaggaa gctctgccgg tgcaatcatg tctttgatgt gctacctagg
    13321 ttatactgct gatgaaatta gtgaactttt caagcatttc aaacaagagc atttagttca
    13381 atttgatata gacagaaatg gattatctga tactcattcc ttaaagaccg cgctggatta
    13441 tgctatcgca aataaagtaa ggcaaatagt aaataaatat aaaattccat atcctaaggg
    13501 aaaaataact ttcagtactt tagcgtctct aagagagcaa tttccagatt gtggaatagg
    13561 caaagaatta attgttacag caacaaataa acgctcagga aaaactattt atttatcagc
    13621 aaatcgttac ccattattgg aggtcagtga agcagttaaa atttctgcga gctttccagt
    13681 tttgtataga gatactctgc tggatggtga agagcataat gacggtggaa tattaagtaa
    13741 ttttccaacc gaggtttttt ttgatgacca ctcaacgttg ttggagtctg aatttggtaa
    13801 taatttaaaa gtattggcag ttcaatttga taatgggatt gaaagaaatg ttattgatag
    13861 aataatggac aaagtttatc gagagaattt cgttcttaac tggatttata gcttattaac
    13921 tggagtgagt gatcctgcaa gtggatggga aagagaccga cttaaattaa gacaatatgc
    13981 tcttcaaact ataattcctg aaacagggaa agtatcaaca acaggattta atgtagagag
    14041 gcagagccag gaggagttga tccaaaatgg ttatcaatca actctggatt atcttaatgt
    14101 aagatatact agaaaaaaca acagctatgt taacaaggaa ataatgtact ctactttcaa
    14161 ttctttggga gatttgcttg cttactgttg ttatagagga gatgaacaat ggtttaatgt
    14221 tgttaataat ctaattgtgc aatctacctt acctaatcgt actgctttaa tgaagcaatc
    14281 ccttgaactt agaaaacttt attttaatac gtctctacca tctaatcctg aaaagaataa
    14341 aacagcttgt actgaaagca atactttaac tttttttggt aatgcggttt gccagcatca
    14401 taatcacagc caggaacttg aaaatggaaa ccatgatgtc ttactggcgc tctatccaat
    14461 ttttttaaaa ctatctactg atttattaat aaataaaatt gataagaacc tattagaccg
    14521 tgcacgtcat tcactaaata tacattctcc ctttgattgt cttgagcatt ttaacaaaat
    14581 ttcaggaaat gctcatgtta tgattcatat catcgttaaa ttaattaaag agctcaaaga
    14641 aagatcatgc caacaaattt atgaggtatt aaaagaggtt aggcatttat tgtatagcga
    14701 cattaatctt atgaaagcaa attattttgg caaatgggat ttaagtctcc ctcaatgtat
    14761 acggatcttg aatttgttaa aactggaaaa gcatgacgat gtatctcggc tattggattg
    14821 cctacgtaac caaatggagc ctgtgcaaac agtaaaagga ggagtgcatc atgatgacct
    14881 taataacggg ggccacgaga ggtataggct tggccttggc gtatgagttt gcatcacatg
    14941 gccatgattt aatacttatt ggtagagatt ctgaacagct ggagcacatt tcaaagcaat
    15001 taaatacaca atatcaagtt aatgttgaat ataaagtaca tgacttaagt aaacctggaa
    15061 gcgcatttaa gttgtatact gatttaaagg agcagggtgt agaagtcaac tgcctggtta
    15121 ataatgcggg cataggctat atgggttctt ttgttgagat gggcatgaac aagctcgatg
    15181 atttaatgaa cataaacatg atttctttaa gtgaactaac actatgtttt cttcatgatt
    15241 ttgtggctcg aaacgaaggc agtattttac aggtttcttc aacagcagca tttcaaccgg
    15301 gtccttttat ggcagcctac tatgcgagta aggcatatgt ggcaacgctt tcccatgcta
    15361 ttgcttatga gcttaaagat actaaggtat ctatgtctat tctttgtcct ggagcaacaa
    15421 aaactaattt tttccatgct tctggtatgg aaagttctat gttggagagg ggatatattg
    15481 gaatgatgac ccctgaaaag gtcgctaaaa ttgcctataa aggattaaaa aaaggaaagt
    15541 tgtatattat tccggggcta agaaataaaa ttttagccta tgcagcagcc atatcaccca
    15601 aagcgattgc tcttaggatc gcatcttatc ttcatcataa aattttgtag atgatatgcg
    15661 gcttcaattg atgaaaataa caggatttac tctacttgaa acgatgcttg cattagcttt
    15721 gcttttagga ttattgcttt tatgttcatg gtcatttttt tcactaatac aaaacaatga
    15781 gagagaaaca ttagttaata gtataaaaac agccattcaa tactccaaaa tacaggctat
    15841 tcatttgggg catccgattt atcttcttcc ttttggttct aatgaaaatt ggtccagggg
    15901 gatggtgctc gctgaactta atcgaaaatc caataaaacc gagcttattt accaatggca
    15961 atggagttct aattcgtgga atattaattg gaaaggcgta gattcaaacc atagaattat
    16021 tatatccaat attccgaatc gtgcgatgag taatggtaaa tttattttga ataataggcg
    16081 aaccaatgaa aaagttgtgg ttactttaaa tagg
//
LOCUS       DBNCWK010000064        15724 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.064, whole genome shotgun sequence.
ACCESSION   DBNCWK010000064 DBNCWK010000000
VERSION     DBNCWK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15724
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.064"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(265..1623)
                     /locus_tag="AC06IO_001762"
     CDS             complement(265..1623)
                     /locus_tag="AC06IO_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIO9874941.1"
                     /translation="MKLSFLGATGTVTGSKYLVESNNKKILVDCGLFQGLKELRLRNW
                     SPLPVSPESIDAVVLTHAHIDHSGYLPLLVKQGFKGRIYATAATTDLCSILLPDSGFL
                     HEEDARRANKYGYSKHHPALPLYTQEDANNTLKYFESVEYDTPYPLIEDCEISWHRAG
                     HILGSAFIQFQTKQIKLLFSGDIGRLNDPVMKPSELMKSTDYLVIESTYGNRLHDKTD
                     PLAFIANIVNTTCKRGGTVLVPAFAVGRAQTMLYYLYILKQKKLIPDVPVYLDSPMAI
                     DATELLLKHHNEHRLSAEECRAVCQIATYVHTPNESMAVDWERESKIIISASGMMTGG
                     RVLHHLKVYAPDPASTILLTGFQAAGTRGEKMLNHEPSVKIHGDDYPVRAQVAMLTNA
                     SAHADYEEILVWLKHFEKAPKEVFITHGEPQAALGLKEHIEAELGWNCKIPNYLEKVN
                     LD"
     gene            complement(1746..2396)
                     /locus_tag="AC06IO_001763"
     CDS             complement(1746..2396)
                     /locus_tag="AC06IO_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874942.1"
                     /translation="MNYFIGLCFGLLNAVICYAEPNGSSLPKELIKVNKENNPKCVEF
                     VTYKGELYCSVVPLADSSSDSQVIHYEKQIVRFDDRPWKIAWGKKTDKVVTIEYIPVG
                     DDINNWKELITTQFMPGLINMTPAQFGSQFLYNLNKSGVKYIVHVIEEQPNLLIFEFK
                     IQEPANLQQDEILKITKGKDGIYVLHYAIKKSDMTKENRDKWVKNLKSSFIKAFTP"
     gene            2513..2803
                     /locus_tag="AC06IO_001764"
     CDS             2513..2803
                     /locus_tag="AC06IO_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage holin"
                     /protein_id="HIO9874943.1"
                     /translation="MNGCKISPVGLGLSLGILWGLSLLVMGLIAYFYAYGRPFVEAVA
                     TLYLGYEPSVLGSFIGGVIGFIDGFVTGFLIAWLYNCFSCCSCCRGKENKKE"
     gene            complement(2894..3205)
                     /locus_tag="AC06IO_001765"
     CDS             complement(2894..3205)
                     /locus_tag="AC06IO_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIO9874944.1"
                     /translation="MLKCLSSVIIVFLILFTAGCQTNTVSNMFIPSNPSGMTLAQSVK
                     DSLMESNDPVINQIHVESNQNVVILSGYVKKIRQSDIAEQIARQVQGVQSVENRIIVR
                     P"
     gene            3422..5209
                     /locus_tag="AC06IO_001766"
     CDS             3422..5209
                     /locus_tag="AC06IO_001766"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIO9874945.1"
                     /translation="MKKTTKKPINKTKEMPASSKKEILNPLEQTPSPEQSDPIFRFID
                     KLYQANLGKLTAGISPAALGTAYYSWFAQLLQSPGSMLRLAYYPLLHANDYLSNLFKY
                     DKPRDGKDVRFHTENWSYYPWRLWAEQFLQFEDWCLQASSKVPGIPLHVKRTVTFSTR
                     QILDALSPSNFVLTNPDLLQETIRSNGQNLIRGTELAFQDFVEKITGSPPAGVENFIP
                     GKQVAVTKGKVVYSNHLIELIQYTPQTEKVYKEPILILPAWIMKYYILDLLPENSLVN
                     WLVRQGHTVFIVSWRNPTKEDRNLGLDDYYKLGAMDAINAVSNSIPHTKIHLMGYCLG
                     GTMALLTAAAMAHDHDNRLKTLSLLAAQGDFIDAGELLLFITKSEVSFLKSMMWEQGY
                     LDTKQMSGTFQMLRSYDLIWSKMVQDYMHGTQRGMIPLLAWNADATRMPYKMHSEYLE
                     KLFLNNDFAEGRFILDGKPVVGENIRIPAFVVSTEKDHVAPWKSVYKTHLLINSDITF
                     VLTNGGHNAGIVSEPGHEGRYYRIRERKMDSTYLDPTNWLKKAELREGSWWIAWHDWL
                     VNHSSQKQVSAPKLDKNLPNAPGKYVLQK"
     gene            5221..6627
                     /locus_tag="AC06IO_001767"
     CDS             5221..6627
                     /locus_tag="AC06IO_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HIO9874946.1"
                     /translation="MNNEFLENVTYEELTIGRKASLNRTLTQDDINLFAAMSGDINPS
                     HVDPQFAKSDVFHGVVGHGMWSGALISTLLGTALPGPGTIYLEQDIQFITPVRIGDNL
                     TITIIVRDKHPGKSIVHFDCMGVNQKGETVIKGLAKVIAPTKKMKVPRTVLPLVQIHH
                     NDHFNKIIEACSNLPSIKTAVVHPVTANVLEAVYDSVKAGLITPVLIGPMAKIKCAAS
                     EAKIDLSDWEMIDTEHSDAAAFKAVELAASGKVDSIMKGALHTDELMSAVVPVTSGLR
                     TKYRISHAYVMDVPTYHKPLIITDAAINIAPNAADKADICQNAINLWRILYGEEIKPK
                     VAILAAVEMVNPKMQATVDAAVLCKMADRGQIIDGILDGPLAFDNAINKQASKEKNII
                     SPVAGDADILLVPDIESGNMLAKQLTFLGHADAAGIVLGARVPIILASRADSLRTRLL
                     SCAVATKIAAARKAGKIK"
     gene            6624..7742
                     /locus_tag="AC06IO_001768"
     CDS             6624..7742
                     /locus_tag="AC06IO_001768"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIO9874947.1"
                     /translation="MRHSALLIINSGSSSVKFQVFSNTEKLDLLAKGKIINIGGEPSF
                     TAINETSEHQRTLITLEKDMTYEQAIHLILDWISQQQQNWEIHAVAHRVVHGGEYFTQ
                     SVLVTPQIISQLETLNSLAPLHQPHNLAAIKIIGRIKPELPQIACFDTAFHAQHTPLF
                     TTCALPEYLRQQGIRRYGFHGLSYEWISLVLKKDYPDLANNRIIIAHLGNGASLCAIN
                     RGISIDTTMSLTALDGLPMGTRCGSLDPGAVIYMMRELNLPPGKVEEILYNESGLLGL
                     SGKTNNVLELQSSPDKNAQFALEYFCLKTAQFIGMMAISLGGVDGIVFTGGIGENSEP
                     IRANILKRLECLHPFSVIVIPANEEKMMAMHTLTALRT"
     gene            7782..8672
                     /locus_tag="AC06IO_001769"
     CDS             7782..8672
                     /locus_tag="AC06IO_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIO9874948.1"
                     /translation="MDKDYYKIMGVSQDASEKDIKMAYRKLARKYHPDISKEPDAEEQ
                     FKEMAEAYEVLKDPKKRSEYDQYLKYKEFNPQSDGFTGRRTQEQPFQEFHFDSDFFET
                     LFGHSRYQQQPMTGQNYHGKITISLEEAYHGAVKNLQVPVEQGTETKIQTLKVKIPAG
                     VKSGQQIRLAGQGGSGSGGGARGDLYLTVEVIKHPIFDVMENDIYLTLPITPWEAALG
                     ATVVIPTLAGKIDLKIPPGSQGGQKLRIKNRGLPGSPPGNQYVLLKIITPPAQTEEAK
                     ALYKKMAEVMPYNPRKTMGV"
     gene            8675..8989
                     /locus_tag="AC06IO_001770"
     CDS             8675..8989
                     /locus_tag="AC06IO_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HIO9874949.1"
                     /translation="MSKDNLLIGMLIEETTTISFIEVCQKYHIPEELFIEMIEQGLFP
                     DQPSDPEKIALGQKELRRLESAFRLHRDLGINLPGVALALDLLEKIDQMHHELEILRK
                     HF"
     gene            complement(8986..9549)
                     /locus_tag="AC06IO_001771"
     CDS             complement(8986..9549)
                     /locus_tag="AC06IO_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIO9874950.1"
                     /translation="MQKICRFIFKLIGWKIVGELPNEKKYIVIVAPHTSNWDFVICLC
                     ARFVVGVKINFLAKHQLFFFPLSYFLRAVGGTPVDRSKKGNRVEKVVELFRQRDELKL
                     GLAPEGTRSPVTRWKEGFYHIARQAGIPIVMVGPDYSTKEIRIHEPFWTTGDIKKDFQ
                     YIIEFFRTIKGYRPKEIPDYNPKDSDA"
     gene            9667..10218
                     /locus_tag="AC06IO_001772"
     CDS             9667..10218
                     /locus_tag="AC06IO_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HIO9874951.1"
                     /translation="MLYHDPLISEHTMKWVRTFIINYTICPFAKGPVNKGALRIITSD
                     TLKKHQALKDLITEIQFLEANPAVETTLLIFARAFKDFFSYLDFISLAEQLMQNLNYE
                     GIYQLATFHPDYYFADTSPDDVANYTNRSPYPMIHLLREDSLDKAIAAYGDTHTIPEK
                     NIATMNELGLEKMKRLLASISIP"
     gene            complement(10251..11489)
                     /locus_tag="AC06IO_001773"
     CDS             complement(10251..11489)
                     /locus_tag="AC06IO_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIO9874952.1"
                     /translation="MNSILFKNANVILGESTEIQKNFDVMVQNDLISLVSQTPLQPLE
                     GMRVIDVKGKTLMPGLIDAHAHVTGLTLSPKNIFYSEAEIFLAAATYLKNSLFYGFTT
                     LREAGGADFRIAQLLDNKSIPGPRLFYSGRALTQTGGGADFRKPNEQIDPCGHVGSFS
                     TMSVIADGVDEVRKAAREELRKGATQLKVFASGGVVFPSLSNPTLYEYSEEELSTIVE
                     EARARNTYVMAHAYSDESVRKCIKSGVRSIEHANFVSEPTVELMSESGVFYDPTFISL
                     VQRIESAEQNRLSEAIVANLKNTIEKGKKVYEYALKYKIPIAFGTDLWGPEAQRDQLR
                     EFEMRKELDSAANIIRSATVVNAELLMQKGKLGVISEGAYADLLVVDGNPLVNLNVLI
                     RPDENLKLIMKDGVIYKNEL"
     gene            11691..12251
                     /locus_tag="AC06IO_001774"
     CDS             11691..12251
                     /locus_tag="AC06IO_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874953.1"
                     /translation="MNTQDPALSISNPRLLSAIYFGLLSVVGTILINAFLTSIGIEEI
                     IPVYQSVILGMVVASCTGAIFGEAIVHCKKPYKRKTFWLGFSMVIASLPVFDLGLVFL
                     MSEANAKLFEVTDLGSMVLFYLVVLAYSYVLFGILLAVASGLAAMYLRGQLVYDILHT
                     QTRRQQKQKHALAKNKSEGPAHKVHR"
     gene            12199..14775
                     /locus_tag="AC06IO_001775"
     CDS             12199..14775
                     /locus_tag="AC06IO_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIO9874954.1"
                     /translation="MHWQKINQKDLPIKFTDELKSDELYNLLSQGAVVITPNNRLSGT
                     ILQNYFTYCSRKTITKPICLPFNTFLVNSFEQLKFQKPDLEHPILLNSAQCQHLWRKL
                     IKSHPSIIYTEGLLKTIMQAWEYCEQWQLTPEAPAFHYTPQTQQFQQWWQSFNKILKE
                     ISAIHQHQLIPYLLDSNYQGLVKTVVWMCFDDFNPQQLSLQNYLNAQGVIQYGYDLEE
                     NQETPEVFSAKDKKTEYQELMTWLEKKIQQGKKQIGVVVPNLEQESSVLQRILRDCFE
                     PNLFNISLGKPLSAFHLISHALIWLSLDHNLLNNHQAALLLQSPYLGKAKDEFLQRSD
                     YLQESKLLQDQSFPTKLFIEDIAYHTPKLAELLKNMNFYPEKTTLDEWIELFQERLNG
                     IGFPGDDVLNSEDYQCFNRFTMIFDEFRQLSLISSYLTKKEAFEALKQLIDNTIFQPQ
                     KSNSPIQILGLLEASGCEFDSLWVMGLSDRCLPQQTRLSPFIPFELQRELGMPHSIPA
                     RELQYAKQILQRLQRGSVETVFSYPQFEDDTPNLPCSLITHYPAYLSRPADNKTNVQS
                     CLVPLEENYEVPVKPDESISGGTSLLSNQAKCPFKALAQHRLKANPVLKTTDGMDDKE
                     KGQLIHKILELLWQRLENQEQLIKLKPDELERFIGQAIQGALNPIRQRHSQAFPDLVA
                     NVEYMRLKRIILSYLEWEKQRPPFKITGLEENHSINLAGLDFKVRVDRLDEVGNKQWV
                     IDYKSTLPGGKPWNEERPMEPQLLLYALLNEQINTLLLIQLKTGKIKLSGFSEEKWET
                     KEINTLKEDENWEDYRQRWLEQLTHLAEEIKSGYCPPRPQNLTICQRCDFQNLCRFQA
                     KQ"
     gene            14803..15645
                     /locus_tag="AC06IO_001776"
     CDS             14803..15645
                     /locus_tag="AC06IO_001776"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIO9874955.1"
                     /translation="MHHLILGYGYCGYYLAQELLGHHQQVTVVSRQLKKELELSKLQH
                     ISHDLEYPFHWSEPDSIIYYLIPPPSQGTCDTFLQQFLNQSSLNAKKIIYFGSSGVYG
                     NHQGAWVDELSACIIDSPRQQRRLDAEQQWLAYCQQHNKELSLLRIAGIYGPNRIPVD
                     AAKSKIPIIKPTEAPFTNHIFVKDLALIAYLLGQKQTTSAVYNVADGNPTHMGALQQT
                     VANALGIESASYESFEQAWEKASPMKREFMRASKRLKIDMLKAALGEDIQFTSLHDAV
                     KQSL"
BASE COUNT         4961 a         3292 c         2891 g         4580 t
ORIGIN      
        1 tttgaaagta cttttatatc accaactccc gccgtttaat cgaacaggtt cgggagacaa
       61 ttgaatcaat tggcgatttt ctaacttccg gatcattaag cggaattgac ctctaatcct
      121 ttcaataaaa gccattattt ctgcaccgga aaaatgacac gggaatattg cgaaaaaaca
      181 ggaatatgaa tgattgttcc ctgttttccg agtctctgta gctcttgctc agaatattaa
      241 atcgaatgtt attttgggga ttcgttaatc caaatttact ttttcaagat agttaggtat
      301 cttgcaattc catcctagct ccgcttcaat atgttccttt aaacctaatg ccgcttgagg
      361 ctcgccatgg gtaataaaaa cttcctttgg ggctttctca aaatgcttta gccaaactag
      421 tatttcttca taatctgcgt gagcagaggc attggttaac atagctacct gagccctgac
      481 tggataatca tcgccatgga ttttaacaga cggctcatga tttaacattt tttcacctct
      541 cgttccagca gcctggaatc cggtcaaaag gatagtactt gcaggatcag gagcatatac
      601 cttcaaatga tgcaaaaccc tgccccctgt catcatgcca cttgcagaaa taataatttt
      661 agattcgcgc tcccaatcca ctgccatgga ttcatttggg gtatggacat aagtcgctat
      721 ttggcagacg gctcgacact cctctgcact taagcggtgt tcgttatgat gttttaataa
      781 taattcagtg gcatcgatag ccatagggct atcgagatag acagggacat caggaattaa
      841 ttttttttgt tttaaaatat acaaataata cagcatagtt tgtgccctac cgactgcaaa
      901 agcaggaact agcactgtac cgcctctttt acaagtcgta ttgactatgt tagctataaa
      961 cgctaatgga tcggttttat catgtaatcg attcccataa gttgattcaa tcaccaaata
     1021 atcggtagat ttcattaact cggaaggctt cataacagga tcattgagtc ttccaatatc
     1081 accactaaat aataacttga tttgctttgt ttgaaattga ataaatgcag accctaatat
     1141 atgccctgcc ctatgccaac tgatttcgca atcctcaatt aaaggataag gggtgtcata
     1201 ttctactgat tcaaaatact ttaaggtatt atttgcatct tcctgagtat acaaaggtaa
     1261 agccggatgg tgcttgctgt acccatattt atttgccctt ctggcatctt cctcgtgcaa
     1321 aaaaccgcta tcaggcagca agatagaaca taaatcggtt gttgcagctg ttgcatagat
     1381 gcgcccctta aatccctgtt tcaccaacaa tggcaaataa ccactatgat caatatgtgc
     1441 atgggttaaa acaacggcat ctatcgattc tggagacaca ggcaggggtg accagtttct
     1501 tagtctcaat tcttttaacc cttggaaaag cccacaatct accaaaatct ttttattatt
     1561 tgattctact aggtacttag aaccggtaac cgtaccggta gcgcctaaaa aagacaattt
     1621 cataataata ccttgttgta tgtcattctc agtactgtaa tgaataatac gacctattac
     1681 caatttattt taaagtcatt tgaagtttca aattgtaaat ctttatcccg cccattcact
     1741 tgcgcttacg gtgtgaaggc ttttataaaa ctgcttttta agttttttac ccacttgtct
     1801 ctattctctt tagtcatatc cgatttttta attgcataat gaagcacata aattccatcc
     1861 ttgcccttgg taattttcag aatttcgtct tgttgcagat tggcaggctc ttgaatttta
     1921 aactcaaaaa tgagtagatt gggttgctcc tcaatgacat ggacaatata ttttacccct
     1981 gatttattca agttgtataa aaattgacta ccaaactgag ctggagtcat gttgattaaa
     2041 cccggcataa attgtgtagt aatcaactct ttccagttat taatgtcatc tccaacagga
     2101 atatactcaa tggtaaccac cttatctgtt ttctttcccc aagcgatctt ccatggtctg
     2161 tcatcaaaac gtactatttg cttctcataa tggataactt gtgaatcact tgaggaatca
     2221 gccaaaggaa caacactgca ataaagttct cccttatagg ttacaaactc cacacattta
     2281 ggattgtttt ctttattgac tttgattaat tcttttggaa gagaagagcc attaggctct
     2341 gcgtagcaaa taacggcatt aagtaatcca aaacacaacc caatgaaata attcatgctc
     2401 ttaatcccaa aagtggatac tatttaattg tagacattga aacccatgac ccacaatttt
     2461 gattagacaa actggtatat aattatatta gagagctaat aaggagattg ccatgaatgg
     2521 ttgcaaaatc agtcctgttg gactgggtct ttctttagga attctatggg gattatcatt
     2581 gttagtcatg ggattaatcg cctattttta tgcctatggc agaccgtttg ttgaagcagt
     2641 tgccacctta tacctcggct acgaaccttc tgtcctggga agcttcattg gtggcgtgat
     2701 tggctttatt gatggattcg tcacaggatt ccttatcgct tggctttaca attgttttag
     2761 ttgttgttct tgctgccgag gcaaagaaaa taaaaaagaa taagtttgct aataatgaga
     2821 aatcatgacg tcaatgacgt taaaaccaac gtcattgaca agagcaatta atttattcaa
     2881 tagtcaaaat catttatggg cgaacaataa tacggttctc tactgactga accccttgaa
     2941 cttgtctggc aatttgttct gcgatatcgc tctggcgaat ttttttcaca taaccactta
     3001 atatcaccac attttgattg gattccacat gaatttgatt gatcacagga tcattacttt
     3061 ccattagtga atcctttacc gactgagcca aagtcatgcc tgatggatta gacggaataa
     3121 acatgttact cacagtattg gtctgacaac ctgctgtaaa caaaatcaag aacacaatga
     3181 tgacactgga taaacatttt agcatcacaa cctccaagat agagtggatt tatttacact
     3241 gcattaaaag aaccaaataa tagcacaact ccccgtattt ctatagtcaa gccactgcct
     3301 ataaatcata gtttgccaag agagacttaa gtcttttcaa acgataactc agattaaatt
     3361 gtcatttatg gaatgctgct atactatttt ttataaatca aacacctaca ggacgcaagg
     3421 tatgaaaaaa acaaccaaaa aacctatcaa taaaacaaaa gaaatgcctg catcctcaaa
     3481 aaaagaaatc ctaaatccac tggaacaaac accttctcct gaacaatccg atcccatttt
     3541 cagattcatt gataagttat atcaagctaa cctgggaaag ttaaccgctg gtataagtcc
     3601 tgctgcgcta ggtacagcct attactcctg gtttgctcaa ttgctacagt cacctggttc
     3661 aatgctacga ctggcttatt atccgctgtt gcatgccaac gattatcttt ccaacctttt
     3721 caagtatgac aagcccagag atggcaagga cgtacgtttt catacagaaa actggagtta
     3781 ttatccctgg cggctttggg ctgagcaatt tctccaattt gaagattggt gtttacaagc
     3841 ctcaagcaaa gttccaggta taccacttca tgtcaaaaga acagtaacct tttccaccag
     3901 acagattctt gacgccttgt caccttccaa ttttgttttg actaatcctg atttgcttca
     3961 ggaaacaatt cgctcgaacg ggcaaaattt aattcgcgga acagagcttg cctttcagga
     4021 ttttgtagaa aagataacag gctcacctcc tgcgggagtc gagaatttta tcccaggaaa
     4081 gcaagttgct gtaacaaagg ggaaagtcgt ttatagcaat catttaattg aattaatcca
     4141 gtacacaccc caaacagaaa aggtatacaa agaaccgatc ttgattttac ccgcctggat
     4201 catgaaatat tatatcctgg atttgctgcc ggaaaactct ttagtaaact ggctggttag
     4261 acagggacat actgtattta ttgtatcttg gcgcaatcca acgaaagaag atagaaatct
     4321 tggactggat gattattata agttgggtgc tatggatgcc ataaatgcag tgagcaattc
     4381 cattccacat accaaaatcc atttaatggg ttattgtctt gggggcacca tggctctgtt
     4441 aaccgcagca gcgatggctc atgatcacga taaccgtctt aaaacattat ccttgctcgc
     4501 tgctcaaggc gattttattg atgccggaga gctactgctc tttataacca aaagtgaagt
     4561 atccttttta aaaagtatga tgtgggagca aggatatctg gacacaaaac aaatgtctgg
     4621 aacttttcaa atgctccgtt cgtacgattt gatttggtct aaaatggttc aggattacat
     4681 gcacgggact caaagaggga tgatcccttt actggcatgg aacgccgatg caactcgtat
     4741 gccatacaaa atgcatagtg aatacctgga aaaactgttc cttaataatg attttgccga
     4801 aggccgattt attctggacg gaaaacctgt cgtaggagaa aatattagga tacctgcttt
     4861 tgttgtaagt acagaaaaag atcacgtagc cccctggaaa tcagtgtata aaacccattt
     4921 gttaatcaat tccgatatta cttttgtctt gactaatggg ggtcataacg ctggaattgt
     4981 cagcgaaccg gggcatgagg gacgttatta tcgtattcgg gaaagaaaaa tggattcaac
     5041 ctatcttgac ccaacaaact ggctaaaaaa ggctgaatta agagaaggat cctggtggat
     5101 tgcctggcac gattggttag ttaatcactc atcccaaaaa caggtgtctg cgcccaaact
     5161 ggacaaaaat ttgcccaatg ctcctggaaa atatgtatta caaaaataaa ggaattagta
     5221 atgaataatg aatttcttga aaatgtcact tatgaagaac tgactatagg gcgcaaggcc
     5281 agcttaaacc gtacattaac tcaggatgat attaatttat ttgctgcaat gtctggagac
     5341 atcaatccct cccatgtcga tcctcaattt gctaaaagcg atgtattcca tggtgtagtc
     5401 ggacatggta tgtggtcagg agctctaatt tctaccttgt tgggcactgc ccttcccggg
     5461 cctggaacta tctatcttga acaagatatt caatttataa ctcctgtccg cattggagat
     5521 aatcttacaa ttaccatcat tgttcgtgac aaacatccag gcaaatcgat tgttcatttc
     5581 gactgtatgg gggtgaatca aaaaggagaa acagtaatta aaggccttgc caaagtcatt
     5641 gccccgacta aaaaaatgaa ggtaccccga actgttttgc ctctggtgca aatccatcac
     5701 aatgatcact ttaataaaat cattgaagcc tgcagtaact tgccctcaat caaaacagca
     5761 gttgttcatc ccgtcactgc caatgtcctg gaagcagttt atgactcagt caaggccggt
     5821 ttaattactc ctgtattaat aggtcctatg gcaaaaatca aatgtgccgc aagtgaggct
     5881 aaaatcgatt tatccgattg ggaaatgatt gatacagaac acagtgatgc tgctgctttt
     5941 aaagcagttg aattagcggc atccgggaaa gtggattcta tcatgaaagg cgccctgcat
     6001 actgatgagt taatgtctgc agtggtacct gtcacttctg gactaagaac caaataccgc
     6061 atcagtcacg cctatgttat ggatgttcca acttaccata aacctttaat cattaccgac
     6121 gcagccatta atatagcccc aaacgcagcg gataaggcag atatatgtca aaatgcaatc
     6181 aatctctggc gtattttata tggagaagaa ataaaaccca aggtagctat ccttgcggcc
     6241 gttgagatgg taaatccaaa gatgcaggca acagttgatg ccgctgtcct gtgtaaaatg
     6301 gctgacagag gtcagataat cgatggcata ctggatggcc ccctcgcctt tgataatgcc
     6361 atcaataaac aagccagtaa ggaaaaaaac attatctctc ctgtagccgg tgatgccgac
     6421 attttgctgg taccggacat tgaatcagga aatatgctag ccaaacaact cacttttctc
     6481 ggacatgctg acgcagcagg aatagttctc ggagcaagag tccctatcat tctggccagc
     6541 agggctgatt ccttaagaac acgtttgttg tcgtgtgcag tagcaaccaa gattgcagcg
     6601 gcccgtaaag caggcaaaat aaaatgaggc attctgcact gttaattatc aattcaggct
     6661 cctcaagtgt taaatttcaa gtattctcca atacagagaa acttgatctt ctcgccaaag
     6721 gcaaaattat taatattgga ggagaaccat cttttaccgc aattaacgaa acatcggagc
     6781 accagagaac tctaatcact ttggaaaagg acatgacgta tgagcaagcc attcatctta
     6841 tactggactg gattagccaa cagcagcaaa actgggaaat tcatgctgtc gcccatcggg
     6901 tcgttcatgg cggagaatat ttcactcaaa gtgtcctggt tactccgcaa atcatcagcc
     6961 aattggaaac actgaattct ctcgcgcccc tacaccagcc tcataatttg gctgccataa
     7021 aaatcattgg tcggattaag ccagaattac ctcagattgc atgctttgat accgcctttc
     7081 atgctcaaca tactccatta tttactactt gtgctttgcc cgaatattta agacaacaag
     7141 gaattaggcg ttatggattt catggccttt cctatgaatg gatttctctc gttctcaaaa
     7201 aagactaccc tgatttggct aacaatcgaa ttattattgc ccatttaggc aatggcgcca
     7261 gcttgtgcgc tataaatagg ggaatcagta tagacaccac catgagttta acagcgctgg
     7321 atggtttacc aatgggaact cgttgtggca gtttggatcc tggggctgtt atttacatga
     7381 tgagagaatt gaatcttccg ccaggtaagg tagaggaaat actttataac gaatccggct
     7441 tgcttggttt atcaggaaaa accaataatg tgctggaatt acaatccagc cctgataaaa
     7501 atgctcaatt tgctttggag tatttttgcc tgaaaacagc tcaatttatt ggaatgatgg
     7561 ccatttcgct aggtggagta gatggtattg tattcacagg aggaataggg gaaaattcag
     7621 aaccgattag agcaaatatc ctcaagaggc tagagtgctt acaccctttt tctgttatcg
     7681 ttattcctgc gaatgaagaa aaaatgatgg ctatgcatac cttgacagcc ctcaggacgt
     7741 aaaggcgtat tttttactgg cataggtaat gagtataaaa aatggataaa gactactata
     7801 aaatcatggg agtcagtcag gatgcttctg aaaaagacat caaaatggct taccgaaaat
     7861 tagcccgcaa atatcacccc gatattagta aagaaccaga tgctgaagaa caatttaaag
     7921 aaatggcaga ggcctatgaa gtactgaaag accctaaaaa aagatccgaa tatgatcaat
     7981 atctgaaata caaagaattc aatccccaaa gcgatggatt taccgggcga agaactcaag
     8041 aacaaccctt tcaggaattt cattttgaca gcgatttttt tgaaaccttg tttggacatt
     8101 cgcgctatca acagcaaccg atgactggtc aaaactatca tggcaaaatc actatcagtc
     8161 tggaggaagc ttaccatgga gctgttaaaa acttacaagt tcctgttgaa caaggcacag
     8221 agactaaaat acagaccctg aaagttaaaa tccctgcagg agttaaatca ggacaacaaa
     8281 ttcgcttggc aggacaagga ggcagtggaa gcggaggagg ggctcgcggc gacctgtatc
     8341 taacagtcga ggtgataaaa caccccatat ttgatgtcat ggagaatgat atttatctca
     8401 cattacctat cacaccgtgg gaagctgctt taggagctac ggttgtcata ccaaccctgg
     8461 caggaaaaat cgatttaaaa atccctccgg gctcacaagg cgggcaaaaa ctcagaatta
     8521 aaaatcgcgg attaccaggt tcaccccctg gtaatcaata tgttctatta aaaattatta
     8581 ccccccctgc tcaaactgaa gaagcaaaag ctttatataa aaaaatggcg gaggtaatgc
     8641 cttacaaccc tcgtaagact atgggagtat aagcatgagc aaagacaatc ttttaatagg
     8701 catgcttatc gaagaaacca caacgatttc ttttattgaa gtatgccaaa agtatcatat
     8761 tcctgaagaa ttattcattg aaatgatcga acaaggtttg tttccagatc aaccgtctga
     8821 tcctgaaaaa attgcattag gccaaaagga attgcgtcgc cttgaatccg cctttcgatt
     8881 acatagagat ttaggcatta acctgcctgg agtcgcttta gctcttgatt tactggaaaa
     8941 aattgatcaa atgcatcatg agcttgaaat tttacgcaag catttttagg catcagaatc
     9001 tttaggatta taatcaggta tctctttagg acgataacct ttaatcgttc taaaaaactc
     9061 gataatgtac tgaaaatcct ttttaatatc tcctgtggtc caaaaaggtt catggatacg
     9121 gatttcttta gtggaataat caggccccac catcactata ggaatacctg cttgccgagc
     9181 aatatgataa aatccctctt tccatctggt caccggactt cgggtacctt caggagccag
     9241 gcccagcttt aattcatcgc gttggcgaaa caactctaca actttctcaa ccctgttccc
     9301 ctttttagaa cgatcaaccg gagtgcctcc aactgcacgc aaaaaatagc tcaaagggaa
     9361 aaaaaataac tgatgtttcg ctaaaaaatt gattttaacc ccgacaacaa aacgggcaca
     9421 caggcaaatg acaaagtccc aattgctggt atgcggagca acaatcacaa tatatttttt
     9481 ttcattcggt aattcaccaa ctatcttcca accgattaat ttaaaaataa aacgacatat
     9541 tttttgcatg acaaaaatcc atttttgttt taaccaaagt atacttgatt ttgatatcta
     9601 tttagaagaa attgttattt cacatgatca aggtacaata tccctttttt cgaaaccact
     9661 taccctatgc tctatcatga tccattgatt tcagaacaca ccatgaaatg ggtgcggaca
     9721 ttcattatca attacacgat ttgccctttt gcaaaaggcc ccgtaaacaa aggcgcttta
     9781 agaataatta cttcagatac cctcaaaaaa caccaggcac ttaaagattt aatcactgaa
     9841 attcaatttc tggaagcaaa ccctgctgtt gaaacgactc tgcttatttt tgctcgagca
     9901 tttaaagact ttttttctta tctggatttc ataagccttg ctgaacaatt aatgcaaaac
     9961 ttaaattatg aagggatata ccaactcgct actttccacc cggattatta ttttgctgat
    10021 acttctccag atgacgttgc caactacact aatcgttccc cttatcctat gatccattta
    10081 ttaagggaag acagcctgga caaagcaatc gcggcctatg gcgataccca cactatacca
    10141 gaaaaaaata tagcaactat gaatgagctt ggtttagaaa aaatgaaacg gttattggct
    10201 tccatttcta taccctagta tgcaacagga cttctgaaaa agaccttgct ttataattca
    10261 ttcttataaa tgaccccatc tttcataatc aatttaaggt tttcatccgg gcggatcaga
    10321 acatttaaat tgactaaagg attaccatct actactaaca gatcagcata agctccttcg
    10381 cttatgacgc caagttttcc cttttgcatt aataattccg cattgacaac ggtagctgaa
    10441 cgaatgatat tggctgctga atcaagctcc ttacgcattt caaattctct taactgatct
    10501 ctctgtgcct caggacccca caaatctgta ccaaaagcaa taggaatctt atattttaag
    10561 gcatattcat aaaccttttt gcctttttca atagtatttt tcaaattggc tacaatagct
    10621 tcagacaacc ggttttgttc agcactttca attctttgca ccaatgaaat aaatgtcgga
    10681 tcgtaaaaca caccactttc cgacattaat tctacagttg gttcgcttac aaaattagcg
    10741 tgttcaatgg aacgcacacc cgatttaata cactttctta ctgattcatc gctataagca
    10801 tgagccataa cataagtatt tcttgctcgt gcctcttcaa caatagtcga taactcctcc
    10861 tcggaatatt catacagtgt tggattgcta agggaaggga aaacaacccc gcctgaagca
    10921 aatactttta attgtgttgc tcctttcctt aactcttctc gagcagcctt tctcacttca
    10981 tcaaccccat ctgcaataac cgacatcgtc gaaaaagaac caacatgccc acaaggatca
    11041 atctgttcat tcggttttct aaagtcagct cctccacccg tttgagttaa tgccctcccg
    11101 gaataaaata atcgaggccc tggaatactc ttattatcca gtaattgtgc tattctaaaa
    11161 tcagcgcctc cagcttctcg taaagtcgta aaaccataaa acaagctgtt tttaagataa
    11221 gtagcggcag ctaaaaatat ctctgcttcg gaatagaaga tattttttgg gcttagggtc
    11281 aatccggtaa catgagcgtg agcatcgatt aaaccaggca ttaatgtctt acctttgaca
    11341 tcaataactc gcattccttc gagaggttga agcggtgttt gcgacaccag ggaaatcaaa
    11401 tcattctgta ccataacatc aaaatttttt tgaatctctg tagactctcc caaaataacg
    11461 ttggcatttt taaaaagaat tgagttcatt ttaatctcga attcattaat ctaacttact
    11521 ctatttatag tctattttgg caactccgac tcgctctcat tttcctttac cctactattt
    11581 caaaagtatt taatttaaca ggaccccata gaaaattaat aaaacccgag tccacattat
    11641 tgaaataatg tactaatatt tatcatatag aacttattat aaggtgcatc atgaacacac
    11701 aagatccggc gctttcgatc tcaaatccac ggttattatc agcaatctat tttggcttgt
    11761 tatcggtcgt tggcacgata ctgatcaatg cattcctcac ttcgattgga attgaagaaa
    11821 ttatcccggt ctatcaatcg gtcattcttg gcatggttgt agcttcctgc actggggcca
    11881 tttttgggga agccatcgtt cactgtaaaa aaccatataa aagaaaaacc ttttggctag
    11941 gattcagcat ggtcatagcc tcactgcctg tctttgatct cggccttgtc ttcttgatgt
    12001 cagaagcgaa tgccaagctt tttgaagtaa ccgatctagg cagcatggtt ttgttctatc
    12061 tagttgtttt ggcctatagt tatgtgcttt ttggtatttt gctggctgtt gcatccggac
    12121 tagctgctat gtatttaaga gggcaattag tatacgatat cctgcatacc caaacccgac
    12181 gtcaacaaaa acagaagcat gcattggcaa aaaataaatc agaaggacct gcccataaag
    12241 ttcaccgatg aacttaagtc agatgagctt tataatctat tgtctcaagg agcagtcgtt
    12301 attactccta acaatagatt aagcggaacc atccttcaga attacttcac ttattgttcc
    12361 agaaaaacaa ttaccaagcc aatatgcttg ccttttaata catttctagt taactctttt
    12421 gaacaactca aattccaaaa accagacctg gaacatccca tacttttaaa ctcggcacaa
    12481 tgccaacatt tatggcgaaa actaattaaa tctcatcctt ctatcattta cacggaaggc
    12541 ttgcttaaaa caataatgca agcatgggaa tactgtgagc aatggcaact tacacctgaa
    12601 gcgccagctt ttcactacac gccgcaaaca caacaatttc aacaatggtg gcaatctttt
    12661 aataaaatac ttaaagaaat ttctgccatc catcagcatc aattaattcc ctacttgctt
    12721 gattccaatt atcaagggct tgtaaaaacc gttgtctgga tgtgctttga tgactttaat
    12781 ccacaacaat tatccctgca aaattatttg aacgcgcagg gggtaattca atatggttat
    12841 gatttagaag aaaaccagga gactccggag gttttctcag ctaaagacaa aaaaacagaa
    12901 taccaggaat taatgacttg gttggagaag aaaattcaac aaggaaaaaa gcaaatcggg
    12961 gttgttgtgc ctaatcttga acaagaatca tcggttttgc aacgaatttt acgcgactgc
    13021 tttgaaccca atttatttaa tatttcttta ggcaaaccac taagcgcatt tcacttgatc
    13081 tctcatgccc tcatatggct gagtcttgat cacaatctct taaataatca tcaagcagct
    13141 ttgctgctcc aatcacccta ccttggcaag gcaaaagatg agtttcttca aagatcagat
    13201 tacttacaag aaagcaagtt gttgcaagac caatcttttc ccacaaaatt atttattgag
    13261 gacattgcct accacacgcc taaactggct gaattgttaa aaaacatgaa tttttatccg
    13321 gaaaaaacta ccctggatga gtggattgag ttatttcagg agaggttaaa tggcattggt
    13381 tttcctggag atgatgtttt gaactcagag gattatcaat gctttaatcg ctttacaatg
    13441 atttttgacg aattcagaca attaagtctc ataagcagct atttgaccaa aaaagaggca
    13501 tttgaagcgt taaaacaact catagacaac actatttttc aaccacaaaa aagcaattca
    13561 ccaattcaaa ttttgggatt gttagaggca tcaggttgcg agtttgacag cctttgggta
    13621 atgggattga gtgatcgatg tttacctcaa caaacacgtt tatcgccctt tatcccattt
    13681 gaattacagc gtgaactggg catgcctcac agtatcccgg caagagagct ccaatatgcc
    13741 aagcaaattt tgcaaagact gcaacgaggc agtgtggaaa ccgtttttag ttatccacaa
    13801 tttgaagatg acactcctaa tttaccctgc tcattgatta ctcattatcc tgcctacctc
    13861 agccggcctg ctgacaataa aacaaatgtt caaagttgtt tggttcctct tgaagaaaac
    13921 tatgaagtgc ctgtaaaacc tgatgaatcc atttctggcg gaaccagctt actaagcaat
    13981 caggccaaat gcccttttaa agcattagca caacaccgac ttaaggcaaa ccctgttcta
    14041 aaaactacag atggaatgga tgacaaggaa aaaggtcaat taatccataa gatactggag
    14101 ttactctggc aaagattaga aaatcaggaa caattaatta aattaaaacc cgatgaactt
    14161 gaacgattca ttggtcaagc aattcaaggc gctttaaatc cgattaggca acgacactcc
    14221 caggcttttc ctgacttggt tgccaatgtt gaatatatga gattaaaacg aattatcttg
    14281 tcttatctgg aatgggaaaa gcaacgccct ccttttaaaa tcacaggcct ggaagaaaat
    14341 cattcgatca atttggctgg gctcgatttt aaagtaagag tggacagact ggatgaagta
    14401 ggtaacaaac aatgggtcat tgattataaa agcactctcc ctggcggcaa accatggaat
    14461 gaagaaagac caatggagcc tcaactgcta ctttatgcgt tgcttaacga acaaatcaat
    14521 actttgttat tgattcaatt aaaaaccgga aaaatcaagc tcagcggttt tagcgaagaa
    14581 aaatgggaaa ccaaagagat taatactttg aaagaggatg aaaattggga agactatcga
    14641 caacgctggc tagaacaatt aactcattta gctgaagaaa ttaagtctgg ctattgtccg
    14701 ccacgccctc aaaatctgac catttgccaa cgctgtgatt ttcagaactt atgccgcttc
    14761 caggctaaac aataaaccag ttttaattta tcgggactac ttatgcatca tttaattctt
    14821 ggttatggct attgcggtta ttacctggcc caagagcttc ttgggcatca tcaacaggta
    14881 accgtagtat cacgacaatt aaaaaaggaa ttggaacttt caaaactaca gcatataagc
    14941 catgatctcg aatacccatt tcattggagc gagcccgact ccattatcta ctatctaatt
    15001 cccccgcctt cgcaaggtac ctgcgatact ttcttacaac agtttttaaa tcaaagttca
    15061 ttaaatgcaa agaaaattat ttactttggt tcaagtggag tctatggaaa ccatcaagga
    15121 gcctgggttg acgaattatc agcttgtatt attgactccc ctcgtcaaca aagacgattg
    15181 gatgcagaac aacaatggct ggcctattgt cagcaacaca ataaagagct ttccttattg
    15241 cgtattgcag gcatctacgg accgaacaga atcccagtcg atgcagccaa atcgaaaatt
    15301 ccaataataa aaccgactga agcccctttt accaatcata tttttgtcaa ggatttggct
    15361 ttaatagctt atttgcttgg ccaaaagcaa acaacatcgg ctgtctataa tgttgccgat
    15421 ggcaacccaa cacatatggg cgcactacaa caaacggtag caaacgcatt aggaatcgaa
    15481 tcagcatcct atgagtcctt tgagcaagct tgggaaaaag caagccctat gaaaagagaa
    15541 ttcatgcggg cgtccaaacg actgaagatt gacatgttaa aagcagcatt aggtgaagat
    15601 attcaattta ccagtttgca tgatgcagta aaacagagct tatgattttc tgttttgtaa
    15661 aaaactattc ttgataagtt aaactttaat tttctttaaa atatggtatt atgctgccac
    15721 attg
//
LOCUS       DBNCWK010000065        15396 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.065, whole genome shotgun sequence.
ACCESSION   DBNCWK010000065 DBNCWK010000000
VERSION     DBNCWK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.065"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            16..723
                     /locus_tag="AC06IO_001777"
     CDS             16..723
                     /locus_tag="AC06IO_001777"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIO9874956.1"
                     /translation="MHIQIDGIPIEILRKPIKNLNLRIYPPDGLVKVSVPLKYSEQLI
                     KQQLQEKSEWIRAQCEQIRARSFYREPSLQTGATIPFKGKSYLLIVEEHHGPSQILIK
                     NELIYCYTKPDLTPQQKQNILDRWYRKEMNLVIPRLIQQWEAIMKVKINQWGIKKMKT
                     RWGSCNPRAQRIWLNLNLIKKPLECLEYVLVHEMVHILEASHNKRFYALMTQFMPKWQ
                     EYQRILEGTPNTSTFAR"
     gene            809..1447
                     /locus_tag="AC06IO_001778"
     CDS             809..1447
                     /locus_tag="AC06IO_001778"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIO9874957.1"
                     /translation="MFNPDLIAHNICTQGFHIIDEFLEEAHYCSLRDMAYELKQQNLF
                     RNAKIGQNLQTQQNDKIRSDQIFWIDADSPYPPIQAYLKKLISLGNILNQTMYLGLAE
                     FETHFAIYLPGTFYRKHIDQFESTKTRKISFVYYLNDAWQKEFGGELLLYNIEDELIQ
                     NISPLGNRFVCFDSQLPHEVHPTQRMRFSMTGWMKTRSTSVLADNKLTQITQ"
     gene            1534..2592
                     /gene="vipA"
                     /locus_tag="AC06IO_001779"
     CDS             1534..2592
                     /gene="vipA"
                     /locus_tag="AC06IO_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipA"
                     /protein_id="HIO9874958.1"
                     /translation="MKHYDSMDQELITMPISNAFLKLFSEFVDNYARNGTPSFYHWAK
                     EQNHEVINKFFHELLEIIKSNTETFHDVSIIEHLNATVLLSAKTNPYLLDCWISFITK
                     YNAETNSFTPIGASMHIKVGPEGTPSERIIRELKESLVMTQQAFILAQKANATEVDSL
                     KRQLEQTKQENERLVTQNQFLKDQVRAEKTFADLNPQINNARQQLSALTELIQTLSEI
                     TNAKPKIETVSSLSIPVVTENKSELLKENSCSVASVSKEVTPAICQEPKKRPVPPPPN
                     DMDGQALSPNIPPKEDVSIKVTIPSLPKPNSTQPTAKNSGFFSGDGFLKELNAKIKER
                     KERPKDEPHTTPVEKKNL"
     gene            2823..3737
                     /locus_tag="AC06IO_001780"
     CDS             2823..3737
                     /locus_tag="AC06IO_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9874959.1"
                     /translation="MHALEIKQLSKTYSNGVEALKGIDLTVNTGDFFALLGANGAGKS
                     TTIGLITSLVNKTSGNIKIYGFDLDAYPEKAKYCLGLVPQEVNLNIFENCEQILLNQA
                     GYYGISRKKALPRAESLLEQLGLWDKRRSIVRHLSGGMKRRLMIARALIHQPKLLILD
                     EPTAGVDIEIRHSMWDFLTRTNAEGTTIILTTHYLEEAEQLCKNIAIIDKGQIIKNTS
                     MKALLQTLRHQTFIFNTENPIDYLPDIHPFNVTPIDPTTFELRVDNNFSLNEVFAVLT
                     KQGIKIHSMRNKTNRLEELFMDLIKNGV"
     gene            3727..4500
                     /locus_tag="AC06IO_001781"
     CDS             3727..4500
                     /locus_tag="AC06IO_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9874960.1"
                     /translation="MAFKYQLIALYTLVRRELVRMFRISSQVFLPPVITTTLYFLIFG
                     SLIGPRIGDIDGVDYPQFIAPGLIMMSVIINSYGNVSNSLFSVRFQKSIEEMLISPMH
                     HGLLLLGYVIGGVLRGLIVAILVFVISTFFLPVELDHLPMTLVVVVLVAAIFSLAGFT
                     NAMVARNFDDIMLIPTFILTPLTYLGGVFYSINMLPPFWQKISLFNPILYMVNALRHT
                     MIDQEEVSITLAMIIIFLMLIALTVGNMILLKKGVGFRE"
     gene            complement(4529..5548)
                     /gene="htpX"
                     /locus_tag="AC06IO_001782"
     CDS             complement(4529..5548)
                     /gene="htpX"
                     /locus_tag="AC06IO_001782"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIO9874961.1"
                     /translation="MGIADYHATAGDWRAQLRRNEKKTRRVIAVFFLVYIFVGLIVDA
                     FVLQSMYPHASLENIIHALITFKVVPFATLLMIAFAGISLLITYALYDRIMLLGTEYY
                     QITPETARSLQEKQLYNVVEEMKVASGLQYMPKVYIIEASYMNAFASGYSEKSAMVAI
                     TRGLMEKLDRAEMQAVMAHELSHIRHHDIKLTLMVAVLSNILLIVIDILFYSVIYKRD
                     RRGDNRLLMVIIILRYVLPIITVLLALFLSRTREYMADAGCVELMRDNEPMARALLKI
                     NQDHVQNAEQYAKAYGDTPHEQVRQASYLFDPSHIDPVKSLNNAFSTHPSIEQRLEAL
                     GFKRK"
     gene            complement(5559..6140)
                     /locus_tag="AC06IO_001783"
     CDS             complement(5559..6140)
                     /locus_tag="AC06IO_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIO9874962.1"
                     /translation="MKTFFIAILIIIALAFVWAISIYNTLIGLIEAINNNKKQIDIQL
                     DRRFKVFESLIEAVKKYMDYEKTTLKDVVALRNQAQAAKDAGDEQGRIAAENGISKIA
                     SNLNLVFEQYPDLKASQNVMQLQEEIVNTENKLAYAKQAYNDGIERYNAKKKSFFESM
                     VVAMFSSALDKEFIYWGLPEEQIQAKENYTVKL"
     gene            complement(6324..9179)
                     /gene="uvrA"
                     /locus_tag="AC06IO_001784"
     CDS             complement(6324..9179)
                     /gene="uvrA"
                     /locus_tag="AC06IO_001784"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIO9874963.1"
                     /translation="MHTITIRGARTHNLKNLDLELPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDSIEGLSPAISIEQKATSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPTHHTSLHAQTISQMVDQVLALPEDSKVMILAPVIRERKG
                     EHVQFLQQLQAQGYVRARIDGELYELDSPPKLGLRTKHTIEVVIDRFKVRPDLAQRLS
                     ESFENALNLADGLAIVSSMDGHFKELLFSSRFACSECGYSLSELEPRLFSFNNPVGAC
                     SSCDGLGVDQFFDPSRVVHDATASLAEGAIRGWDRKTTYYFSMLESLARHYGFDTSTP
                     FCDLSEAMQQIVLYGSGREIIEFQYHRPHGGYMVKRHSFEGIIPNMQRRYRESDSGMI
                     REELAKYLSSRPCQSCQGARLREEARHVFIDNKNLPEITSYSIEQAYQFFKELKLSGY
                     RGEIAAKINKEIVERLGFLVNVGLDYLSLARSAETLSGGEAQRIRLASQIGSGLVGVM
                     YILDEPSIGLHQRDNDRLLSTLMHLRNQGNTVIVVEHDEDAIRSADFVLDIGPGAGVH
                     GGEVVASGSPEEVMNNPASLTGQYLSGKKEIEIPKDRIPVNHDRMIHLKGVSCNNLQN
                     VDISIPLGLLTCITGVSGSGKSSLINDTLYPIAANALNRASLMTIGEVKDISGLHLCD
                     KVIDIDQSPIGRTPRSNPATYTGLFTHIRDLFSGTPESRARGYQPGRFSFNVKGGRCE
                     ACQGDGLIKVEMHFLPDIYVSCDICQGKRYNRETLEIHYKGKNIHEVLDMTVEDACAF
                     FAAIPVIARKCQTLMDVGLSYIKLGQSATTLSGGEAQRIKLARELSKRDTGNTLYILD
                     EPTTGLHFHDTKQLLTVLTRLREQGNTIVIIEHNLDVIKTADWIVDLGPEGGSKGGKI
                     IATGTPEMVAENPQSFTGQFLRQILSKSNELLLQKCK"
     gene            9274..9732
                     /locus_tag="AC06IO_001785"
     CDS             9274..9732
                     /locus_tag="AC06IO_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874964.1"
                     /translation="MKSLIKNLLFIVVFSINSSALYAVNSNTRVIQWAQQTLMETLSV
                     SYTSKPQDFVTVRKNYSLNAWSGITGFLGGNMQSIREKQLTLHPVLLPDTQILSTGLY
                     SGMSYWIIRQSVAIDELNTKLTFTLTILGRSGAAGSPYIVQSLSILLNAL"
     gene            9991..10542
                     /locus_tag="AC06IO_001786"
     CDS             9991..10542
                     /locus_tag="AC06IO_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIO9874965.1"
                     /translation="MPKCMHFILATALIFVTSTFYASNLREIEQEVSDSVITAKITAK
                     FAESRHLNPLKISIDTEKGIVTLSGYAPDKQAFIDALQIAKNTKGVKAVHAKDLHIKK
                     VNTAFTDAFITAEVETAVLKAKILDDESIPLVGINASTTNGEVTLSGHVKSARSIAAI
                     IKRVNEIPGVKKIVTNLEIQENT"
     gene            10539..11012
                     /locus_tag="AC06IO_001787"
     CDS             10539..11012
                     /locus_tag="AC06IO_001787"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIO9874966.1"
                     /translation="MSPLTQFKFFLWFFSHFKVALIGYLKPKLIQLTDNEIVVRLPLT
                     RRSRNHLHSMYFGALAVGADIAGGLHGFYHAKQAKCKVSLAFKSFQAQFLRRPESDVY
                     FICTEGDVVKKMIEESKKSGERINKPIHIKAYINYLTHPEEIADFILELSLKVIK"
     gene            11207..11920
                     /locus_tag="AC06IO_001788"
     CDS             11207..11920
                     /locus_tag="AC06IO_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIO9874967.1"
                     /translation="MIDLIIQTQPDDETCGPTSLHAIYLYYGLDLPLNEIIQGVDKSL
                     SGGTLTPMLGKHALLHGFRTTVYIYNLNIFDLTWFHNGEVNNSFLMDKLEAQMKYKND
                     PYITKASMAYLDYLKLGGTIRSKSLNVDLLKNYFNKKIPILTGLSATYLYRSAREYFT
                     PEGKSVYDDIRGEPCGHFVVLCGYDVKKRLVIVADPHAENPLSHNNYYKVSINRLINS
                     IMLGVLTYDANLLIIEPKE"
     gene            11924..13378
                     /locus_tag="AC06IO_001789"
     CDS             11924..13378
                     /locus_tag="AC06IO_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HIO9874968.1"
                     /translation="MQTVIVTDDLDSWSFLSELAPIVHALEYLSGDEYHQNKSFRVIN
                     LCRSYNYQSIGYYVSLLAQARDHKALPSVHSIQDVLNVSLSKLISQDTHEEIQHSLHD
                     IKGNEFVLSLYFGQNLAKRYATLAKQLHGLFPLPLIRFYLEKKKQWRIKTVVPLSLSD
                     VPEHHLEFMRQSAENYLSKKRVHQWRKKQRFHDLAILVDPSEPNAPSNKKALELFVSC
                     GEELGLNVDIIDKNEAKAIAEYDALFIRATTSVNHYTYRVSRRAAQENLVVIDDPQSI
                     IKCSNKVYLAELLSSHQIMTPDTLFISKYDKELPKIEFPCVLKKPDSAFSHGVVKIDD
                     EKSLQKSLNQFFKNSDLVVVQPFIPTEFDWRIGILDNKALFACRYFMAKGHWQIYDWH
                     CQEENKEGDSETLPLHEVPEQIIKIALKSTRLIGDGLYGVDIKSHGNKYYVIEVNDNP
                     NIDHGLEDKILGQNLYEQIMSVFLQRIRRKHGYV"
     gene            13371..14591
                     /locus_tag="AC06IO_001790"
     CDS             13371..14591
                     /locus_tag="AC06IO_001790"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIO9874969.1"
                     /translation="MSDFPIFSVLGIEIEYMLVDKESLNVQPKSDLILRALAGHQVNE
                     IELGDIAISNELVLHVLELKNNGPKPLHVPVAEHFQKTIRELQPLLAQHNLLLLPTGA
                     HPWMDPNTETIRWPHGNHDIYNQFNSIFNCQGHGWANLQSMHINLPYSNEEEFCQLHN
                     ATRLILPLLPAIAASTPFLDGQATGLLDSRLYFYSRNQQRIPSISGDIIPEFIRTERE
                     YQEQILEPMYRDISPYDPEGILQYQWLNSRAAIPKFDMKAIEIRILDSQECVEADIAI
                     AKAINAILKYWQSTSNFYLDNPCDTKSLKYVFDETIKNGLSCHIDNKKLLEQWQLPLQ
                     GMSVNAIWSLLIERISHELDRKQQLALELILKQGNLSERILKACSSCVNHDSLKQVYT
                     QLAHCLLRNEQFTP"
     gene            14572..15315
                     /locus_tag="AC06IO_001791"
     CDS             14572..15315
                     /locus_tag="AC06IO_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIO9874970.1"
                     /translation="MNNLPPDIMKNIALLISCEHAVNTVPYEYQTLFEPHKAILDSHR
                     GIDFGALEIAQSIHKNNHCSLFQATCTRLLVDCNRSINHPHCFSEITKNLSSDKKKKI
                     MDEFYFPFRNRVMDHIHQLISQGLQVWHLSTHSFTPVMNNIIRATDIGLLYDPRSNSE
                     KILARQWQKEIQILHPQLKVRMNYPYKGISDGFTTSLRKKYSYHEYIGIELEVNQKLV
                     FHNHSLDTLKNTLPLSVSNTIKAYQKSER"
BASE COUNT         4987 a         3169 c         2727 g         4513 t
ORIGIN      
        1 taagcatatt ttgctatgca cattcaaatt gatggcattc ctatagaaat actgagaaaa
       61 cccattaaaa atttaaattt gagaatttat ccacccgatg ggttagtgaa agtgagcgtc
      121 cctttaaaat acagcgaaca gctaataaaa caacagctgc aggaaaaaag tgaatggatt
      181 cgtgcccaat gcgagcaaat tcgcgcacga tctttttatc gtgaaccatc gcttcaaaca
      241 ggtgcaacca tcccattcaa aggtaaaagt tatttattaa ttgttgaaga acatcatggc
      301 ccttctcaaa tcctgatcaa aaatgaatta atttattgct atacaaaacc cgatttaact
      361 ccacagcaaa agcagaacat tctggatcgc tggtacagaa aagaaatgaa tcttgtcatt
      421 cccaggctta ttcaacaatg ggaagccata atgaaagtca aaattaatca atggggtata
      481 aaaaaaatga aaacccgctg gggttcgtgt aatcctcgcg cacaaagaat ctggctcaat
      541 cttaatttaa ttaaaaaacc tttggagtgc cttgaatacg ttcttgttca tgaaatggtg
      601 catattttgg aagccagcca taacaaacga ttttatgcac tcatgacaca attcatgccc
      661 aaatggcaag aatatcaaag gatactggaa ggcacaccta ataccagtac atttgcaagg
      721 taatcattga attcattacc tcattatttc caaaaaataa ttcttggcgt acaatgtatt
      781 atcactttgc ctgattggtg tagacaggtt gtttaatccg gacttaatcg cccataatat
      841 ttgtactcaa ggctttcata tcattgatga atttctggaa gaggcacact actgctcttt
      901 acgagacatg gcttatgaat taaaacagca aaatttattc agaaatgcca aaattggcca
      961 gaacttacaa acgcagcaaa atgacaagat tagaagcgat caaattttct ggatagatgc
     1021 agactcccct tatccaccca ttcaagctta cttaaaaaaa cttatctctt tgggcaatat
     1081 tctgaatcaa accatgtatc ttggtttagc agaatttgaa actcattttg caatttatct
     1141 ccctggaacc ttttatagaa agcacattga tcaatttgaa agtacaaaaa ccaggaaaat
     1201 atcatttgtc tattatttga atgacgcttg gcaaaaagaa tttggtggcg aattgttact
     1261 ctacaatatt gaagatgagc tcattcaaaa catttctcca ttgggaaatc ggttcgtctg
     1321 ttttgatagt caattaccgc acgaagtcca cccaactcag cgaatgcgct tcagtatgac
     1381 aggctggatg aaaacacgct caacctccgt actggccgac aataaactga cccaaataac
     1441 ccagtagcac tttatacgct gttctataaa aaaatgaata attatttttt aacttcacaa
     1501 cagctattga actttaattt aagttgatat taaatgaaac actatgattc catggaccag
     1561 gagttaatta ccatgcctat cagtaatgcc tttcttaagt tattcagtga atttgttgat
     1621 aattatgcgc gcaatggcac cccttcattt tatcattggg caaaggaaca aaaccatgaa
     1681 gtcattaaca aatttttcca tgagcttttg gaaattatta aatcaaacac agaaacattt
     1741 catgatgtca gtatcataga gcaccttaat gctacagtac tattatcagc aaagaccaat
     1801 ccctacttac tcgattgttg gatttcattt attactaaat acaatgctga aaccaacagt
     1861 tttaccccaa taggggcttc gatgcacatt aaagtcggac cagaaggcac gccatccgaa
     1921 cggataataa gggagctcaa agaaagtctg gtcatgacgc aacaagcgtt tattttagct
     1981 caaaaggcca atgctacgga agttgattct ttaaaaagac aacttgagca aacaaagcaa
     2041 gaaaacgagc gcttagtcac acaaaatcag tttctaaagg atcaagtcag ggcagagaaa
     2101 acatttgcag accttaaccc acagattaac aatgctcggc agcaactcag tgctttaaca
     2161 gaattaatac aaactctaag cgaaatcaca aatgccaaac ccaaaataga aactgtatct
     2221 tccttatcca ttccagttgt tacagaaaat aaatctgaac ttctaaagga aaattcatgc
     2281 tctgtcgctt ctgtatccaa ggaagttact cctgcaatct gtcaagagcc gaaaaaaaga
     2341 ccagttccac cgccccccaa tgatatggat ggtcaagcat tatcccccaa tataccccct
     2401 aaggaagatg tgtctataaa agttacaata ccgtccttgc ccaaaccaaa ttcaacccag
     2461 cctactgcaa aaaattcagg tttcttttca ggcgatggtt ttttaaaaga gttaaatgca
     2521 aaaattaaag aaagaaagga acgcccaaaa gatgagccac ataccactcc cgtcgaaaaa
     2581 aaaaatctct agttaagcca atttctgttc ctcctgaata tagggcactc ttagctaaaa
     2641 tggaggaaat tagaaatgca gtaacaggaa aatatcctca taccaatgat tcatcgccag
     2701 agtcaactcc cccttcatcc ccagaattaa cccgttggaa gtgagggatg aattaaaatc
     2761 cataggctac tgtagaataa aaattatggt gtataatagc gtttttactt aagtaataaa
     2821 tcatgcatgc cttagaaatt aaacaacttt caaaaaccta ctcgaatgga gttgaagcgt
     2881 taaaaggcat tgatttaaca gtgaataccg gtgatttttt tgccctgtta ggagcaaatg
     2941 gcgctggcaa atcgactacc attggtttaa tcacatcttt agtcaataaa acctcaggaa
     3001 acatcaaaat ttacggcttc gacctggatg cttatcctga aaaagccaaa tattgtttgg
     3061 gactggtacc tcaagaagtc aatcttaata tttttgaaaa ctgcgaacaa attttactga
     3121 atcaagctgg ttattatggc atttcacgaa aaaaggcttt gcccagagca gagtccttgt
     3181 tagaacaatt aggactctgg gacaagagac gttctatagt tcgccattta tccggtggaa
     3241 tgaagagaag gcttatgatt gccagagcgc tgattcatca acccaaattg ttgattctgg
     3301 atgaacccac cgccggtgtt gatatagaga taagacacag tatgtgggat tttttaacaa
     3361 ggactaacgc agaaggaaca acgattatcc tgacgactca ttatttggaa gaagcagaac
     3421 aattatgtaa aaacatcgca ataatcgata agggtcaaat tattaaaaat acctcgatga
     3481 aggctttatt acaaacattg cgccatcaaa cctttatctt taacacagaa aaccctattg
     3541 attatttgcc ggatatacac ccgttcaatg tgactccgat tgatccaact acttttgaat
     3601 tgcgtgttga caataacttc agtttaaatg aagtattcgc tgtattaaca aaacagggaa
     3661 taaagattca cagcatgcgc aataaaacca accggttaga agaacttttt atggatctta
     3721 ttaaaaatgg cgtttaaata tcaactcatt gccctttata ctctggtaag acgtgaactg
     3781 gtccgtatgt ttcgcatttc gagccaagtg tttttaccac cagtcatcac tacaacgtta
     3841 tattttctaa tttttggcag tttgattggg cctcgtattg gtgatattga tggcgtggat
     3901 tacccacagt tcatagcccc cggattaatt atgatgtctg ttattattaa ttcctatgga
     3961 aacgtctcta attcactatt cagtgtaaga tttcaaaaaa gcatagagga aatgctgatt
     4021 agtcccatgc atcacgggct tttattatta ggttatgtga taggaggtgt gcttagaggc
     4081 ttaatcgtcg ccattctggt ttttgttatc tctacctttt ttctgcctgt tgaactagac
     4141 catttaccga tgaccctggt agtcgtcgtg cttgttgcag ccatattctc gctggctgga
     4201 tttaccaacg ccatggtcgc tcgaaatttt gatgacatca tgctgatccc tacctttatc
     4261 ctgacccccc ttacttatct gggaggagta ttttatagta ttaatatgct gccacccttt
     4321 tggcaaaaaa tctcactttt caatccaatt ttatacatgg taaacgcttt aagacacacc
     4381 atgatcgatc aggaagaagt tagtattact ctggctatga ttattatttt cctgatgtta
     4441 attgcattga cagtaggaaa tatgattctt ttaaaaaaag gggttggatt tcgcgaataa
     4501 aaatatttgg atttcttaca aaaccgatct atttgcgttt aaaacctaat gcttccaggc
     4561 gctgttctat actgggatga gttgaaaagg cattatttag cgattttaca gggtcaatgt
     4621 gagaaggatc aaaaagatag gaagcttgcc ttacctgttc atgaggggta tctccataag
     4681 cttttgcata ttgctccgca ttctgaacat ggtcttgatt aattttaagc aaagccctgg
     4741 ccataggctc attatctctc attaattcga cacaaccggc gtcagccatg tattctctgg
     4801 ttcggcttaa aaagagagct agtaaaacag tgataatagg taagacatat cttaagatga
     4861 taataaccat taacaagcgg ttatctcctc ttctgtctcg cttgtaaatt acggaataaa
     4921 acagaatatc aatgacaatt aataaaatat tactgagcac ggcaaccatg agagtcaatt
     4981 taatatcatg atgtctgata tggcttaact catgagccat tacagcctgc atttcagccc
     5041 tgtccagttt ttccatcaat ccacgagtaa tcgcgaccat cgccgatttc tcactgtaac
     5101 cactggcaaa agcattcatg taactggctt cgataatgta aactttaggc atgtattgca
     5161 agcctgatgc aactttcatc tcttcaacaa cattgtacaa ttgtttttct tgtaagctac
     5221 gagctgtttc tggcgttatt tgataatact ccgtccccaa taacataatg cggtcataaa
     5281 gagcataagt aattaacaag gaaatgcctg caaatgcgat cattaataac gtagcaaaag
     5341 gcaccacttt aaaagtgatt aaagcatgga taatattttc caacgaggca tgaggataca
     5401 tgctttgtaa aacaaacgca tcgactatca atcccacaaa gatataaact aaaaaaaaga
     5461 ctgcaatcac ccttcgggtc tttttctcat ttcgcctcaa ttgagcacgc caatctcctg
     5521 ctgtggcatg ataatctgca atccccatgg tatattgctc aaagtttcac agtgtaattt
     5581 tcttttgctt gaatttgctc ttctggaagc ccccaataga taaattcttt atccaaagca
     5641 gaagaaaaca ttgccacaac catggattca aaaaatgatt ttttcttggc gttataacgt
     5701 tcaatcccat cattataagc ttgtttcgca taagctaact tgttttctgt gttaacaatt
     5761 tcctcttgta actgcatgac attttggcta gctttcaaat caggatactg ttcaaaaacc
     5821 aaattcaaat tggatgcaat ttttgaaatt ccattttctg cagcaattcg accttgctca
     5881 tctcccgcat ctttagcagc ctgggcctga ttacgtaaag caacgacatc ctttaatgtt
     5941 gttttttcgt aatccatgta ttttttaact gcttcgatca gagattcaaa aactttaaat
     6001 cgcctatcca attgaatatc tatttgtttt ttgttgttat taattgcctc tatcaaaccg
     6061 attagagtgt tatagatgct aattgcccaa acgaatgcga gtgcgataat aattaaaatt
     6121 gcgataaaaa atgttttcat cttcctatcc tgtgaaaggt tccttcccta atttatagct
     6181 cgaaatttca aaaaataaca gctaagataa gaaaaaagaa acaactggat aacgtatttt
     6241 ttgtcacaaa aagagattca ggttgcctga aaaacaacct ggatagcaca tcaagtatta
     6301 aagacaaatt gataattaat gaactatttg catttctgta atagaagctc atttgatttt
     6361 gataaaattt gtcttaaaaa ctgaccggta aaagattgag ggttttcagc aaccatttcg
     6421 ggtgttcctg ttgcaataat ctttccccct ttactgcctc cttcaggacc aagatcaacg
     6481 atccaatcag ccgtttttat cacatccaaa ttatgttcaa taatgacaat ggtatttcct
     6541 tgctctcgca agcgagtcaa cacagttaat agctgtttcg tgtcatgaaa gtgtaatcct
     6601 gttgtgggct catcgagaat atacagtgta tttccggtat ccctttttga taattctcta
     6661 gccaacttga tcctttgagc ttctcctcca gagagagtag ttgcactttg tcccagcttt
     6721 atataagaca aaccgacatc cattaatgtt tggcattttc tggcaataac aggaatggca
     6781 gcaaaaaaag cgcacgcatc ttcgacagtc atgtctaaca cttcgtgaat atttttgcct
     6841 ttgtagtgaa tttccaaagt ttccctattg tatcttttgc cttggcatat atcgcaagaa
     6901 acgtatatat caggtaaaaa atgcatttca accttgataa gaccatcacc ttgacaagct
     6961 tcgcaccggc caccttttac attgaaacta aagcgcccag gttgataccc tcgtgctctt
     7021 gattccggag tccctgagaa taaatctctg atatgagtaa ataaaccagt ataagttgcc
     7081 ggattggaac gcggggttct gccaattgga ctctggtcga tgtcaataac cttgtcacaa
     7141 agatgcagtc cagaaatgtc cttaacctca cctatagtca ttaagctggc tctgtttagt
     7201 gcattggcag caataggata taaagtatca ttaatcaaac tggatttacc tgaaccagaa
     7261 acacctgtaa tacaagtcaa caaacctaaa ggaatactga tatcaacatt ttgtaaatta
     7321 ttgcaagaca cgccttttaa atgaatcatc cgatcatggt taaccggaat tcgatccttg
     7381 ggaatttcta tttccttttt cccagacaaa tattggccgg tgagagatgc gggattattc
     7441 atgacctctt caggagaacc gctggcaact acttccccac catgcactcc agccccaggt
     7501 cctatatcaa gaacaaaatc agcgcttcgg atagcatctt catcatgttc cacaacgatt
     7561 acagtgttcc cctggttgcg taaatgcatt aaagtactca ataatcggtc gttgtcacgt
     7621 tggtgcagcc ctattgaagg ctcatccaaa atatacatca cccctacaag ccctgaacct
     7681 atttgactag ccaggcgaat acgttgagct tctccgccag atagagtctc cgcgcttcga
     7741 gccaaagaca aataatctag cccgacattc accagaaacc ccagccgctc aacaatttcc
     7801 ttattgattt ttgctgcaat ttcaccccta tatcccgata acttcagctc tttaaaaaac
     7861 tgataggctt gctcaataga atagctggtg atttctggta gatttttatt atcaataaat
     7921 acatgtcgag cttcttcacg taatcttgct ccctgacaag attggcaagg gcgtgaggat
     7981 aggtatttgg caagttcctc ccttatcata ccagaatctg attcacgata gcggcgttgc
     8041 atgttaggaa ttattccctc aaaactatgc cgcttcacca tgtatcctcc atgcggccta
     8101 tgatactgaa actcgattat ttcacgacca ctaccataca aaacgatttg ttgcattgct
     8161 tcacttaaat cacaaaaagg cgtactggta tcaaaaccat agtgcctcgc aagcgactca
     8221 agcatgctga aataatacgt ggttttccta tcccatcccc tgattgcccc ttctgccaaa
     8281 ctggcggttg catcgtggac tactcttgac ggatcaaaaa actgatccac tccaagccca
     8341 tcacatgagg aacacgcacc gacaggattg ttaaatgaaa aaagccttgg ctcaagttca
     8401 cttaaactgt aaccgcattc agagcatgca aacctggaag aaaataaaag ttccttgaaa
     8461 tgaccatcca ttgaagaaac gatggcaagg ccatctgcca gattgagcgc attttcaaaa
     8521 gattcactca aacgttgagc caaatcaggg cgcactttaa aacgatcaat aactacttca
     8581 atagtatgct tggtgcgcaa tcctaattta gggggagaat ccagttcata aagctctcca
     8641 tcaatacgtg cacgtacgta cccctgcgct tgcaattgtt gcaaaaactg gacatgctct
     8701 cccttccgtt cacgaataac aggcgccaga atcatcactt tactgtcttc tggtaaagcc
     8761 aatacctggt caaccatttg gctaatggtc tgagcatgca agctggtgtg gtgggtagga
     8821 catcttggct ctccgactcg ggcataaagc aagcgcaaat agtcataaat ttcggtaatg
     8881 gtgcctaccg tggatcgggg attatgagag gtagcctttt gttctataga aatcgcaggc
     8941 gatagcccct ctatcgaatc aacatcaggt ttttccatca tggacaaaaa ttgtcgagca
     9001 taagcagaca aagattccac ataacggcgc tggccttcgg catagagagt atcaaaggct
     9061 aaagacgact tcccagaacc agacaatccg gttattacga ttaatttatc tcgaggaagc
     9121 tccaggtcca agttcttgag attatgggtt cttgcgccac gaatggtgat ggtatgcata
     9181 aaattcgcta atttgactta aaaatacatg agtatatctc tgctataata ataaaacaaa
     9241 ttgcacacta tgatgcgaaa tagggatttt aatatgaaat cactaataaa aaacttactt
     9301 ttcattgtgg tattttcaat caactcatct gctctctatg ctgtcaatag taatacccga
     9361 gtgattcaat gggctcaaca aacccttatg gagactttat ctgtcagtta tacttcaaag
     9421 ccacaagatt ttgtcactgt tcgcaaaaat tactcactta atgcctggag tggaataacc
     9481 ggatttttag ggggtaatat gcaaagcata cgcgaaaaac aactaacact tcatccggtt
     9541 ttactgcccg acacccaaat tctctcaaca ggtctttatt cgggtatgag ttattggatt
     9601 atcagacaat ccgtagccat tgatgaactc aataccaagc tgacttttac cttaaccatc
     9661 ctgggacgat ctggggctgc aggctctcca tacatagtgc aaagcttaag tatattgctc
     9721 aatgcattat aattacaaat taaacaggaa tcacctgaaa tatggattgt ctccatgacc
     9781 acatggaatc atcaacatga aaggtatact ctcgctttaa attctgcttc aatataaaca
     9841 ggacttacga aaaaccccaa ggtcgaggca aaaagttttt ttaatgagag agtttagata
     9901 cactaaatga ccgaattaaa aaaaattttt aacaaagaga ttggggtttt tcgtaagtcc
     9961 tgataaaagc aacaactgga ggacacaacc atgcctaagt gtatgcattt tattttggct
    10021 actgcattaa tttttgttac aagtacattt tatgccagca atctacggga aatagaacaa
    10081 gaagtaagtg attcagtgat tactgctaaa attaccgcaa agtttgccga aagcaggcac
    10141 ttgaatccat taaaaatttc aattgacaca gaaaagggaa tcgtgaccct aagtggctat
    10201 gctcctgata aacaggcttt catagatgcc ttgcagatcg ccaaaaacac aaaaggtgtc
    10261 aaggcggttc atgccaaaga tctccacata aagaaagtca ataccgcttt cacagatgcc
    10321 ttcatcacgg cagaagttga aactgctgtt ctaaaagcca aaattctgga tgatgaatca
    10381 ataccattgg ttggaatcaa tgccagcaca acgaatggcg aggtcacctt gtctgggcat
    10441 gtaaaaagtg cgcgctccat tgccgccatc attaaacgcg taaatgagat acctggcgtc
    10501 aaaaaaatcg ttaccaattt ggaaattcag gaaaatacat gagtccatta acccaattta
    10561 aattcttttt atggtttttt agccacttta aagtagcctt gattggttat ttaaagccta
    10621 agttaattca gcttaccgat aatgaaattg ttgtgcggct gccattgaca agacgcagcc
    10681 gcaatcatct gcattccatg tattttggtg ccttggcagt tggtgcggat atagctggag
    10741 gattacatgg tttttatcat gctaaacaag cgaaatgtaa agtatccctg gcatttaaat
    10801 cttttcaagc ccagtttctg cgtaggcctg aatccgatgt ttattttata tgcacagaag
    10861 gggatgtagt taaaaaaatg atagaagaat caaaaaaatc cggtgaacga attaataaac
    10921 caattcatat caaagcttat atcaattatt tgacccatcc agaagaaatt gctgatttta
    10981 tccttgaact ctctctaaaa gttataaaat aacagcgttc gattattata caaatattaa
    11041 ccctacaaat gaaaattgtt ttttcaatct cattttctta aatatcacat gattatcagt
    11101 cttctaaaat cacttattat tgaattgatt acaccatttg acaaagaagc cactcatgtc
    11161 taattttaaa tcatgatggc gctattttta ccgcaaggat ttgcctatga ttgacctgat
    11221 aatacaaact caacctgatg acgaaacttg cggaccaacc agcttacacg ccatttattt
    11281 atactacggt ttggatttgc cattaaacga aatcattcaa ggagtggaca agtctctttc
    11341 aggaggcacc ttaacaccta tgttgggaaa acatgccctt cttcatggat ttagaacaac
    11401 tgtttacatc tataacctga atatatttga cctcacctgg tttcataatg gtgaagtcaa
    11461 taactccttt ttgatggata aacttgaagc tcaaatgaaa tacaaaaatg acccttatat
    11521 caccaaagct tctatggcct atttagacta cttaaaactg ggaggaacaa ttcgctccaa
    11581 atcactgaac gtggacttgt taaaaaatta ttttaataag aaaatcccca ttttaactgg
    11641 cttaagtgct acttacctct atcgcagtgc aagagaatac tttactccgg aaggcaaatc
    11701 agtttacgat gatattcgtg gtgagccttg tgggcatttt gttgtactgt gcggttatga
    11761 cgtaaaaaaa cgccttgtaa ttgttgctga ccctcatgca gaaaacccac tgtctcataa
    11821 caattattat aaagtaagca tcaatcgctt gattaactct ataatgcttg gtgtactaac
    11881 ttatgatgcg aatctactca taattgaacc taaagagtga taaatgcaaa cagttattgt
    11941 tactgatgat ctggatagct ggtctttttt aagtgagctt gccccgatag tacatgcctt
    12001 ggaatatctt agtggggatg agtatcatca aaataaatca tttcgggtca ttaacttatg
    12061 ccgatcttat aattaccaat cgattggcta ttatgtatct ttacttgcac aagcccgtga
    12121 tcacaaagca ttaccctctg tacacagtat tcaggatgtt ttaaacgtca gtttatccaa
    12181 actgatctct caggatactc atgaagaaat acaacacagt ctccacgata tcaaaggcaa
    12241 tgaatttgtt ttaagcctct attttggcca aaatctggca aagcgatatg caacattggc
    12301 aaaacaactc catggtcttt ttcctttacc actcattcgc ttttatctgg aaaagaaaaa
    12361 gcaatggcga attaaaacag tagttccttt gtctttgtcg gatgtaccag agcatcatct
    12421 ggagtttatg cgccaatcag ctgaaaatta tttatcaaaa aagagagttc atcaatggcg
    12481 taaaaaacaa cgatttcatg atttggccat tcttgtcgac ccctccgaac ccaatgcccc
    12541 atccaataaa aaagcccttg aactttttgt ttcttgtggt gaagaactgg gattaaacgt
    12601 tgacatcata gataaaaatg aagcaaaagc aatcgctgaa tacgacgctc tttttatcag
    12661 agcaactact tccgttaatc attatactta tcgcgtgtca cgacgcgcag cccaggagaa
    12721 tttggtcgtt attgatgacc cacaatctat aattaaatgc tccaacaaag tttatcttgc
    12781 agagctctta agcagtcatc aaatcatgac gccagacacc ttattcatca gcaaatatga
    12841 taaggaatta cccaagattg aattcccctg tgttttaaaa aaacctgaca gcgccttttc
    12901 tcatggcgtg gttaaaatag atgatgaaaa atctttacaa aaatctctta accagttttt
    12961 taaaaattct gatttggtag tggttcaacc ttttataccg actgaatttg attggcgcat
    13021 cggtatcctt gataataaag cactttttgc ctgccgctat tttatggcaa aaggtcactg
    13081 gcaaatctat gattggcatt gccaggaaga aaataaagag ggggatagtg aaacactacc
    13141 ccttcatgaa gtgccagagc agattattaa aatagctctg aaaagtacac gtttgattgg
    13201 tgacggatta tatggtgtcg atataaaaag tcatggcaat aaatactacg ttatagaagt
    13261 gaacgataat cccaatattg atcatggcct tgaagataaa atactaggcc aaaatctgta
    13321 tgagcagatc atgagtgtct tcttacaacg aattcgcagg aaacatggtt atgtctgatt
    13381 ttcctatttt ttctgtcttg gggattgaaa tagaatatat gctggtggat aaagagagtt
    13441 taaatgtcca acctaaaagc gatcttattc taagggcttt ggcaggtcat caggttaatg
    13501 aaatagaatt aggagacatt gcgataagca atgaattagt attgcatgtc cttgagttaa
    13561 aaaataatgg tcccaaacca ctacatgttc cagtagctga acatttccag aaaaccatta
    13621 gagagctcca gccccttctt gctcaacaca atttattgct attacctact ggcgctcatc
    13681 cctggatgga cccaaatact gaaacaattc gctggcctca tggtaaccat gatatctata
    13741 atcaatttaa ttcaattttc aattgccagg gacatggctg ggcaaacttg caaagtatgc
    13801 atatcaacct gccttattcc aatgaagaag aattttgtca attgcataac gccacacgct
    13861 tgattttacc cttattaccc gccattgcgg caagtactcc attccttgat ggccaagcta
    13921 caggattact ggattcccgc ttgtactttt atagtcgcaa tcagcaacgc attccctcca
    13981 tcagcggcga tattattcct gagtttatta gaacagaaag ggaataccag gaacaaattc
    14041 tcgaacccat gtatcgagac attagccctt atgatcccga agggatatta caatatcaat
    14101 ggctaaattc tcgtgctgca attcccaagt ttgacatgaa ggctattgaa attcgtattc
    14161 ttgacagcca ggaatgtgta gaagcagaca ttgccatagc aaaagccatc aacgccattt
    14221 taaaatactg gcaaagcacc tcgaattttt atcttgataa tccatgcgac accaagagtt
    14281 taaaatacgt ttttgatgaa accataaaaa acggattaag ttgtcatatc gataacaaaa
    14341 aattactgga gcaatggcaa ttaccccttc aagggatgtc agtcaatgcc atctggtcac
    14401 tattgatcga aagaattagt catgaactgg ataggaaaca gcaactggct ctggaactta
    14461 ttttaaaaca gggaaatttg agtgaaagaa tcctcaaggc ttgtagctcc tgtgtcaatc
    14521 atgattcgtt aaaacaagtg tatacgcaat tagcacattg tttgcttcgt aatgaacaat
    14581 ttaccccctg atataatgaa aaatatagcc cttctaatca gttgtgaaca tgctgtcaat
    14641 acagttccct atgaatatca aaccctgttt gaacctcata aagccatatt agactctcat
    14701 agaggaattg attttggtgc gctggaaatt gcacaatcga ttcataaaaa caaccattgc
    14761 agtttgtttc aagccacctg tacaaggctc ttggttgatt gcaataggag tatcaatcat
    14821 ccccattgtt tttccgagat tacaaaaaat ttatcatcag acaaaaagaa aaaaataatg
    14881 gatgaattct attttccgtt tcgcaatcgc gtcatggacc acattcacca actgattagc
    14941 caaggattgc aagtttggca tctgtccaca cacagtttta ctcctgtaat gaacaacatc
    15001 attcgcgcga ctgacattgg ccttctatat gaccctcgga gtaattcaga aaaaatcttg
    15061 gccagacaat ggcaaaaaga aatccaaata ctccaccccc agttaaaagt acgtatgaat
    15121 tatccttata aaggaatcag tgatggattt actacatcct tgcgtaaaaa atacagttac
    15181 catgaataca taggcattga actggaggtc aatcaaaaac ttgtctttca caaccacagc
    15241 ctggacacac tcaaaaatac actgccattg agcgtatcaa acacgattaa ggcttatcaa
    15301 aaatcagaga ggtaatcgac aacatttttt tcaagttgca atttaaaaaa accaagtggc
    15361 tgaattaaaa aattttttta caaaaaagat tgagtt
//
LOCUS       DBNCWK010000066        14809 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.066, whole genome shotgun sequence.
ACCESSION   DBNCWK010000066 DBNCWK010000000
VERSION     DBNCWK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14809
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.066"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..368
                     /locus_tag="AC06IO_001792"
     CDS             <1..368
                     /locus_tag="AC06IO_001792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874971.1"
                     /translation="MAKIYIAKERITKKGENKLNGRNEKAYIIVTKGNSAPELHHYDP
                     AAKIISKLDLKENHSHELLSIVMQTIGLENIKQGINLFNLDDRNQKEKLDDIILSIED
                     GTNKQELTSAKSIRYHITK"
     gene            complement(462..863)
                     /gene="panD"
                     /locus_tag="AC06IO_001793"
     CDS             complement(462..863)
                     /gene="panD"
                     /locus_tag="AC06IO_001793"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIO9874972.1"
                     /translation="MAYRKMLKSKIHRACVTQADLDYEGSITISPELLKAANILPYEA
                     VNVWNITAGTRFETYAITGEKGSTDICVNGAAAHLVTPGDLVIIASFTQILEEDCAAH
                     EPTVVFVDQFNRLKEIRPERIGVKNKIPCSA"
     gene            1199..1969
                     /gene="rsmA"
                     /locus_tag="AC06IO_001794"
     CDS             1199..1969
                     /gene="rsmA"
                     /locus_tag="AC06IO_001794"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIO9874973.1"
                     /translation="MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGA
                     LTQPLLQKLNRLTAIEIDTDLQSYLTCLPVSQGKLNLIPADALTVDFCQFGPHLRVVG
                     NLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAAAHGTKAYGRLSVMLQYHCEV
                     EYLFDVPPEAFEPRPKVDSAIVRLTPHRVSPFESVNTEKLENIVAKAFAMRRKTLTNN
                     LKGIISLSQLNDLGIDGGKRPEQISVAEYVQLAKFISN"
     gene            2006..3343
                     /locus_tag="AC06IO_001795"
     CDS             2006..3343
                     /locus_tag="AC06IO_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer nickase/helicase
                     domain-containing protein"
                     /protein_id="HIO9874974.1"
                     /translation="MFHRQRKSSLSAQSKSLKDLIRMVTAEQFLSEEKRQVLLQKMRA
                     LSGLEASRYESLCFVLVENLVNYCQNLPETANSYYSQPGGLVDHALNRTEAALSLFKE
                     FTVQEQPDLLSEEQKLWQYALYSAAILQGLGKLFDEFRINLFDSNGQLLKQWNPLLES
                     LIQTGSYYDYQFEKEADVEFRCRLNLLLARTLMPASGFAWIASNPEVLAVWLALLNED
                     YRSAGTLGAILIRADAIAIQRYFLAFMARSGARRSGRYGRAVTFTGGVPESITEKEQA
                     IGVEFIQWIIKSLDEGRIMVNKAPLFMVPGGMLMCPEMFQLFVREHPEYKNWQAIQNG
                     FLSLGLHGRDAGGNVTSRFEQAHTQQMYTGVVFAGYGVALPNSVQVHHLNTGKIESMS
                     ALELIHKSQYTSQFTQQSSAIAIAPLQRLAANGQWQNIEQDMSQLTLGAKRGA"
     gene            3336..4166
                     /locus_tag="AC06IO_001796"
     CDS             3336..4166
                     /locus_tag="AC06IO_001796"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIO9874975.1"
                     /translation="MPDYAIGDVQGCYDPLQRLLELIDFNEKEDCLWFVGDLVNRGPD
                     SLAVLRFIYSLPVKPKITLGNHDLHLLGLLFGGQPWKGHDDTLEEVMLADDGEELGHW
                     LRKQSLLCRSSELNIVMCHAGIAPLWDLSKAVDLANELEAVLSGDSYHEFFAQMYGNK
                     PDIWSDDLVGLDRLRVITNYFTRMRYCDAHGRLDLGYKGTLSKAPNHLYPWFEVPCRK
                     EIEMDIVFGHWAALMGRSSHPRIHAIDTGCLWGGQLTALRLQDRQRFSVPGYGVSRFE
                     "
     gene            4210..5838
                     /locus_tag="AC06IO_001797"
     CDS             4210..5838
                     /locus_tag="AC06IO_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIO9874976.1"
                     /translation="MKFLNKSTALLPVIILSGLISFHASSAKTSSNADNSLYSWTENP
                     SAEAQRVEQAEERLKAAIANPEYMLKNWIELPTSPESKKKTRHQLTIREAILLALRYN
                     PNIQNAELDRIIQRYQLRLANNEFELQYALGASGVVQKSTFEGIGSSTNHSFLASPEL
                     DLKTKIGTKASVSIDNNVSTNNSYNPVLNLSITQPLLRGFGKSANEAKLLDAIDKEWL
                     NKLSLQQSVMDQITQVITAYRALILSGNNLENQRLQLEEARKSYRINEKKIAAGQLEP
                     TGNIQQSYQIESLSLMVEQGENDFKLASQELLQMIGLDPDLHISVPSDVTIKEIIVPD
                     LQESIAIALSHNTQYLAQKMALRADERAYTVAKNQQLWQLDLAGTVQGGAVNDVTGTS
                     SGLSSIYNGNNITESARVTLTIPLNDIGRRSQLINAKVQLEKDKINLIAAKRALITSV
                     TNTINNIQSLAKRYELAQKQVKLAEQSYALEKKKQQAGISSALDVNNTQNQLIQAQSG
                     LIGAKIAYLNQLSALQRILGTTLDYWQIKLRYGG"
     gene            5835..6980
                     /locus_tag="AC06IO_001798"
     CDS             5835..6980
                     /locus_tag="AC06IO_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9874977.1"
                     /translation="MNNYLKIFSMVSLILMLVSCGSSDKPQTSSLKTYEVKPQPLHKT
                     LHFTGTVQPIRESSLTSPMEAVVETMHFHYGQMVKKGEIVLTLNSNELQKQYNDTLTD
                     YLKAKDSYSIAKAKFVGTEELWNAGLISKNNYLSEKSGLDTARVTLMQATRKLSEMLE
                     KMDDKNTQNISNLSLADFDKVRKILTSNHNLIHLKAPSDGIMLYPPKSGEDKNNRVTV
                     GSTIKSGQVIALIGDLKGISVEIDVPEIDIDKIRPGMEATISGVAFGRHKLKGKLVAV
                     NAQASNTSTGGLPSFTAVVEVNSLTPEQQSWIKVGMSASIELNVESNNQLLIPIAAVK
                     REKGSSVVHVQLAKGKVQKRMITTGAAQADSVVVESGLKSGDVVVYD"
     gene            6973..7662
                     /locus_tag="AC06IO_001799"
     CDS             6973..7662
                     /locus_tag="AC06IO_001799"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9874978.1"
                     /translation="MIDRPLIQLSDLVKTYHLEGISTTVLKAVSLTVNEGDLLAIVGA
                     SGSGKSTLMNIIGLLDKPDAGTYTLNNRNVASLSDDEAADLRNQNIGFVFQQFNLLPR
                     FTAMQNVALPLTYRGVNPTLIKEKVEQALDKVGMLQYIRHRPTQLSGGQQQRVAIARA
                     LVTDPQVILADEPTGALDSRTGSEVMNLFLALHQEGRTIIMVTHDEHVAAQCKRRITL
                     ADGAIIAESGQ"
     gene            7659..8852
                     /locus_tag="AC06IO_001800"
     CDS             7659..8852
                     /locus_tag="AC06IO_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9874979.1"
                     /translation="MNFLNHCQQALVNLTASKLRSLLAVLGILVGTAAVVALISCGQL
                     ATEKALEQFKALGTDLLAVSVYQKVPSKSHSESDSLTIDQWQQIAERIPYVLRIAPYS
                     TAYQTLSFNGKVMQGAVIGADESLADIIHVTLAQGHFVSFLDSFEHFCVIGDSLAQQI
                     KEITLDDPIGKQLRIGQSLYTIIGVAERWKENGFFNEDINQAVIIPLAGISLVSKDAK
                     INNAVLLLKPDSPIDEVINEIKEIINSQAPKLSVFPRSAKQIIASMENQGRIFTLLLA
                     VIGGISLLVGGIGVMNVMLVSVSERKKEIGIRKAVGAKNREIQALFLVESVMLSLLGG
                     VLGVVLGLIFTRIVAYFSDWTFTIYLLPPIAGFLVSAATGIFFGFYPARRASKLEPMV
                     SLRSE"
     gene            complement(9009..9335)
                     /locus_tag="AC06IO_001801"
     CDS             complement(9009..9335)
                     /locus_tag="AC06IO_001801"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIO9874980.1"
                     /translation="MNEQLNHLYGLPSHAIEALKCVFKEYPQIDNAILYGSRAKGTYH
                     QGSDIDLCLTGNLLGITELLAIENKIDDLLLPWKVDISLKHTIDNPDLLEHIERAGIL
                     FYTKES"
     gene            complement(9328..9747)
                     /locus_tag="AC06IO_001802"
     CDS             complement(9328..9747)
                     /locus_tag="AC06IO_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HIO9874981.1"
                     /translation="MTNIDVRWQQRLNNYASALQQLSLAVNLAQTRPLSDLEKQGLIQ
                     TFEFTHELAWNVMKDYFFFQGNSAITGSRDATRESFNKGLIKEGEIWMEMIKSRNQTS
                     HTYNQSVADEIVKNIINSYHTSFQAFLEKMQGLKEHE"
     gene            complement(9981..11159)
                     /locus_tag="AC06IO_001803"
     CDS             complement(9981..11159)
                     /locus_tag="AC06IO_001803"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIO9874982.1"
                     /translation="MHIKNIFTWSLMGAVILSTPLFAGEQVKLKSIDSLIDKYIEKYK
                     NEELVTGVGATFLSGTEIISTAHGFENNKRKKRLTNNAYFQIGSITKSFTAALILKLE
                     AEGKLNINQTIGDWLPEYDDWKGITIKQLLNMTSTIPGYATIPTLADSITDNPKKQWT
                     SKELVDLAYGYKHPTSGYNYSDTNYLILGMIIEKVTHDSFHNQLQEKILKPLQLKETY
                     YITDSYTPHFLKKMPESYYYGRTVENLKHGQKATNDNLSMAGPAGAIVATTSDVAKWI
                     KALFSSDFLLPKQQMELKSLISMKTGTPISHVDSEDPNGFGLGVVQGYKPQLGKYWYY
                     EGDTVGFRVLYIYHPCNEKIVVIALNSSPFVDEAAGDHSGDLLLELYKNAFGISQKCE
                     "
     gene            11712..12674
                     /locus_tag="AC06IO_001804"
     CDS             11712..12674
                     /locus_tag="AC06IO_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIO9874983.1"
                     /translation="MKKWGLAIVALMLFNQITYASQSWNQWVAGVREEALQQGIPPSL
                     FDEAFAGIHEPSRKIKGFLRSQPEHRLTFMKYRNTRADNYRIVIGRKEYKRNKEILEE
                     IAQQYGVNPCFIVSFWGMETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHI
                     LKGGHVSLADFKGEWAGASGQPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANY
                     MKQNGWQAGEPWAIQVKLPAKFDMKLEGKSIVKPVSEWNALGVRTESGEPLPYQNLQA
                     SIVQPYGGPTFLAFPNYKMILRYNNSIYYAGAIGYMADKICQGQ"
     gene            12979..14025
                     /locus_tag="AC06IO_001805"
     CDS             12979..14025
                     /locus_tag="AC06IO_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9874984.1"
                     /translation="MKHKSFQWSVVGAGPAGIAAVGKLLDYGIAPSEILWIDPHFKVG
                     DFGQLWRHVSSNTTVKLFSSFLHAANSFSYKKAPSTFRLNNLDPDNTCLLSDVAEPLQ
                     WVTSVLVNQVIAEETVIHSLSLSGGCWTLCSDTQQYRAKNVILATGAVPSSLNYPGVN
                     VVPFEIAIDKQRLAHVVDTNETYGVFGSSHSAIMILRSLIELGVKKVINFYRSPCRYA
                     INMGNWILFDNTGLKGDTAAWARKHIDGVLPANLVRYYPSELNIARHLPECDKVIYAV
                     GFERRKNMTIDNYEHVTPNPHVGIIGPGLFGLGIAYPELSVDPFGTIEHQVGLWKFMV
                     YLCKVLPVWFKYPA"
     gene            14135..14638
                     /locus_tag="AC06IO_001806"
     CDS             14135..14638
                     /locus_tag="AC06IO_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9874985.1"
                     /translation="MIKILIYLGLFLSSNLLMAKSLSQLDSANLVPCFNIEQAERIGK
                     QINKLLQHEFCEENINPKKFASISHNILPKIMTETFLGVTPPENWQQLSDDIIKNCIE
                     NKNLCKKAARKELEECIKPRIPLILIQFGPWLAQNCPQLNKSLIEQWPNKQAILKKII
                     NENKSVE"
BASE COUNT         4346 a         2854 c         3244 g         4365 t
ORIGIN      
        1 ttatggcaaa aatttatatt gctaaagaaa gaattaccaa aaagggtgag aacaagctca
       61 atggaagaaa tgagaaggct tatattattg taactaaagg aaacagtgcg ccggaacttc
      121 atcattacga tcctgctgca aaaataatta gcaagctgga tttaaaagaa aatcattcac
      181 atgaattatt atcaatagtg atgcaaacga tcggattgga aaatattaaa caaggcatta
      241 atcttttcaa cttggacgat cgtaatcaaa aagaaaaatt agacgatatt attctttcga
      301 tcgaagatgg tacaaataaa caagagctaa caagtgctaa atcaatacgt tatcacatta
      361 cgaagtaact tctgcatcat gtgagagttt tgcattagcc tttccctgga ttaagcttta
      421 cctaatccag gctatgatga acttggtgtt ggtaatcaat cttatgccga gcaaggtatt
      481 ttatttttaa ctccgattct ttcaggtcgg atttctttca gacgattgaa ttgatcaaca
      541 aaaaccacag tgggttcatg tgccgcacaa tcctcttcca gaatttgcgt aaaagaggcg
      601 ataatgacca aatcaccggg ggttaccaaa tgggctgctg ctccattcac gcaaatatca
      661 gttgaacctt tttcaccggt aatggcataa gtttcaaatc gggtcccggc cgtgatattc
      721 caaacattaa cggcttcata aggcagtata ttcgcagctt ttaaaagctc tggtgaaata
      781 gtgatactcc cttcataatc caggtccgcc tgcgtgacgc aagcacgatg aatttttgat
      841 ttaagcattt ttctataagc cattatttac ttcctaaatt tgagcctgga aaaagcaatt
      901 gatcaaatac gactaagtct atttgataca tggctttttt cagattgaaa taattaaaaa
      961 aaatagataa gaatatcaac cttggtcatt gtggttgaat tttaccccat attttggagt
     1021 ttacgctata taaatttgta aagttgagag agatgaattt gagttggtga gtattgccgg
     1081 ggtttgctgg cacttgacac taacctacgg taaaattaac aggcttctcc aatggggtat
     1141 accttgtgat gtcaacaaga ttactgaatt aattttatcc taacataggt gatagaaagt
     1201 gaggcatagc ccacgtaaac gttttggtca aaacttctta caagacaaat atattattaa
     1261 tgaaattcta cgcgctatca atcctttggc ggatgataat atgctggaga ttggccctgg
     1321 tttaggcgct ttaactcagc cattattgca aaaactgaat cgattaacag ccattgaaat
     1381 cgatacggat ttacaaagct atttaacctg cttgcccgta tctcaaggca aactgaattt
     1441 aattcctgct gatgcgttaa ccgttgactt ttgtcaattc ggcccccact taagagtagt
     1501 gggtaattta ccttataata tttctactcc attgctgatc tatttattga aatttataac
     1561 ttgtattgat gacatgcatt ttatgttaca aaaagaagtt gtagaacgaa tagcagcagc
     1621 ccacggcact aaagcctatg ggcgactaag cgtgatgttg cagtatcatt gtgaggtaga
     1681 gtatttattt gatgttcctc cagaagcgtt tgagcctagg cccaaggtag actcagcaat
     1741 agttcgatta acacctcatc gtgtttctcc atttgagtct gtaaacactg agaaactgga
     1801 aaatattgtt gctaaagcat ttgctatgag acgcaaaact cttactaata atttgaaagg
     1861 gattatctca ttatctcaac tcaatgattt aggcattgat ggaggtaagc ggccagaaca
     1921 aatttcggtg gcggaatatg tccaactagc gaaatttatt tccaattagt gtaaaattat
     1981 atgataagtt attgtggagg ttgtcttgtt tcatcgtcag cgtaaaagct ctttatctgc
     2041 tcagtccaag tcattaaaag atttaatccg catggtgacc gcagagcagt ttctgagtga
     2101 agagaaacgc caggtattac tccagaaaat gagggcatta agcgggttgg aagcatctcg
     2161 ctatgagagt ttatgttttg ttcttgtcga gaatctggtg aactattgtc aaaatttgcc
     2221 ggaaaccgcg aacagttatt attcccagcc aggtggtttg gtcgatcatg ctttaaacag
     2281 aacagaagcc gcattgagtc tattcaagga gtttaccgtt caagaacagc cagatttgct
     2341 ttccgaggaa cagaagcttt ggcaatatgc tctttattca gcggcaattt tacaaggcct
     2401 tggcaaatta tttgatgagt ttcgcattaa tttgtttgat agcaacggtc aattgttaaa
     2461 acaatggaac cctctattag aaagcttaat ccaaactgga agttattatg attatcagtt
     2521 tgagaaagaa gcggacgtgg agttccgctg ccggctgaac ttgttgctgg ccagaacttt
     2581 gatgccggcc agtggttttg cctggattgc ctccaatccg gaagtattgg ctgtttggtt
     2641 agctttactg aatgaagatt atcgttctgc tggtactttg ggcgctattt taattcgggc
     2701 tgatgccatt gcgatacagc gctattttct ggcgtttatg gcacgaagtg gggcaagacg
     2761 aagcggccgt tatggcagag ctgtaacttt taccggcggt gtaccggaat ctataacaga
     2821 aaaggaacag gcaatagggg tcgaattcat ccaatggatt atcaagtcgt tggatgaagg
     2881 tcgaattatg gtgaacaaag ccccgttgtt tatggttcct ggcggcatgc tgatgtgccc
     2941 tgaaatgttt caattgtttg ttcgtgagca tcctgaatat aaaaattggc aagccataca
     3001 gaatgggttt ttatcattag ggcttcatgg ccgggatgcg gggggcaatg taaccagtcg
     3061 ttttgaacag gctcatactc agcaaatgta taccggggtc gtttttgcag gatatggggt
     3121 tgctttgccc aactctgtcc aggtgcacca tttgaatacg ggcaaaatag agtccatgtc
     3181 agcactggag ttaattcata aatcacaata caccagccaa ttcacacagc aatcaagtgc
     3241 gattgcaata gcgcctttgc agcgattggc agccaatggc caatggcaga atattgagca
     3301 ggatatgtcc caattgacac ttggagcaaa acgcggtgcc tgattatgca ataggggatg
     3361 tacaaggttg ttatgatcct ttacaacggt tgcttgagct aattgatttt aacgagaagg
     3421 aagattgctt gtggtttgtt ggggacttgg taaaccgcgg tcctgattca ttggctgtat
     3481 tacggtttat ttattccttg cctgtgaaac ccaaaataac cttgggtaat catgatttgc
     3541 atttattagg cttattattc ggcggacaac cctggaaagg gcatgatgat acacttgaag
     3601 aggtgatgct ggctgatgat ggagaggaac tggggcattg gttgcgcaag caatcccttc
     3661 tttgcaggtc atctgagtta aacatagtca tgtgtcatgc aggtatcgcg cctttatggg
     3721 atttatcgaa ggccgtggac ctggctaatg aattggaagc ggtgctttca ggcgattcat
     3781 atcacgaatt tttcgcccaa atgtacggca ataagccgga tatttggtca gatgatttgg
     3841 ttgggctgga tagattacga gtcattacca attattttac ccggatgcgc tattgtgatg
     3901 cccatggacg attagattta ggttataagg ggactttatc caaagccccg aatcatctgt
     3961 atccctggtt tgaagtgcca tgccgaaaag aaattgaaat ggatatcgtt tttggtcatt
     4021 gggcggcgct catggggcga agttctcatc cgcggattca tgcgatagat accggatgtc
     4081 tttggggtgg gcaattaacg gcattacgcc tgcaggacag gcagcgcttt tcggtacccg
     4141 ggtatggggt tagtcgtttt gaataacctt gatggatagg catttttata aatacaggcg
     4201 ataacaacgg tgaaattttt aaataaaagc acagcattgt taccggtaat tattttatcg
     4261 ggcctgatta gttttcatgc ctcaagtgcc aagacttctt caaatgctga taattcactt
     4321 tattcctgga cagaaaatcc ttcagctgaa gctcagagag tggagcaagc ggaggaaaga
     4381 ctaaaggcag ccattgccaa cccggaatac atgctgaaaa attggataga gttgcctacc
     4441 tcgcctgaat caaaaaagaa aacaagacat cagttgacga taagagaagc tatactgttg
     4501 gcattgcgtt ataatcccaa tatccagaat gcggaattag accgtattat tcagcgctat
     4561 caattgcgtt tggctaataa tgagtttgaa ttgcaatatg ctcttggtgc ttcaggcgtt
     4621 gtgcaaaaaa gtacttttga ggggataggc agttctacga accattcttt tctggccagt
     4681 cctgaattgg atttgaaaac caaaataggc accaaggcgt ccgtatccat tgataacaat
     4741 gtttccacca acaacagcta taatccggta ttgaatttat ccattactca gcctttgctc
     4801 cgtggatttg gcaagtcagc gaatgaagca aaactgctgg atgcgattga taaggaatgg
     4861 ctaaataaat taagtttaca gcaatcggtt atggatcaga ttacccaggt gattacggcg
     4921 tacagggctt tgattttaag tgggaataat ttagagaatc agcgcttgca attggaagaa
     4981 gccagaaaat cctatcgtat taatgaaaaa aagattgcag cgggacaatt agaacctacc
     5041 ggaaatattc agcaatccta tcagatagaa tctttgagtt tgatggtaga acaaggtgaa
     5101 aatgatttta aattagcctc tcaggaatta ttgcaaatga ttgggcttga tccggatttg
     5161 catatttcag ttcccagtga tgtgacgata aaggaaatta tagtgcctga cttgcaagaa
     5221 tccattgcca tcgcattaag tcacaatact cagtatctgg cccagaaaat ggctttgcgt
     5281 gcggatgaga gggcttacac tgttgcaaaa aatcagcaac tttggcagct tgatttggca
     5341 ggtactgtgc aaggtggcgc agtcaacgat gtcaccggaa ccagcagtgg tttaagcagc
     5401 atttacaatg ggaataatat cacggaatca gccagggtca ccctgaccat tccattgaat
     5461 gacattggac ggcgtagtca attaattaat gctaaagtcc agttggaaaa agacaaaatc
     5521 aatcttatcg cagccaaaag agctttaatt accagtgtaa ccaataccat caataatatt
     5581 caaagcttgg ccaagcgcta tgagctggca caaaagcaag tcaaattggc agagcagtct
     5641 tacgctttgg aaaagaaaaa acagcaggca ggaatatcga gcgccctgga tgtgaacaat
     5701 actcagaatc aattgattca ggcacagtca ggattgatag gggcaaaaat tgcgtatctc
     5761 aatcagttat ccgcgttgca aaggatttta gggaccacgc tggattactg gcagattaaa
     5821 ctaaggtatg gcggatgaac aattatctga aaatattctc tatggtgtca ttgattttaa
     5881 tgctggtttc ttgtggcagt tctgataagc cgcaaacctc ttcattaaaa acgtatgaag
     5941 tgaaacctca acctttacac aagaccttgc attttaccgg gaccgtgcaa cccataagag
     6001 aaagcagcct gaccagtcca atggaagccg ttgttgaaac catgcatttc cattatggcc
     6061 agatggttaa aaagggagag attgttttaa ctttaaattc caatgaattg caaaaacagt
     6121 ataacgacac attaacggat tacttaaagg cgaaagacag ttattccatt gccaaggcaa
     6181 aatttgtagg aaccgaagag ctgtggaatg ccgggttaat ctccaaaaac aattacttaa
     6241 gtgaaaaatc aggcttggat acagcgcggg ttactttaat gcaggccacc cgcaagcttt
     6301 ctgaaatgtt ggaaaaaatg gatgataaaa acactcagaa tatatccaat ctcagtctgg
     6361 ctgattttga caaagtgagg aagattctta cgtcaaatca taatctgatt catttaaagg
     6421 ctcccagtga tgggataatg ctgtaccctc ccaaatcagg agaggataaa aacaatcgag
     6481 tgaccgtagg atcaaccata aagtcaggac aagtcattgc cttgataggc gatttgaaag
     6541 gaatcagtgt ggaaattgat gtcccagaga tagacattga taaaattcgc ccgggaatgg
     6601 aggcaacaat tagtggcgtt gcatttggca ggcataagct gaaaggtaaa ttggtggcgg
     6661 tgaatgcgca ggcatcgaat accagcacgg gaggattacc ctcctttacc gctgtagtgg
     6721 aggtcaattc cctaactcct gaacaacagt cgtggatcaa agtggggatg agtgcctcca
     6781 tagagctgaa tgtggaatcg aataatcaat tgttaatacc tatcgcggcg gttaaaaggg
     6841 aaaaaggcag cagcgttgtt catgtccaat tagccaaggg gaaagttcag aaacgaatga
     6901 ttactaccgg tgccgctcaa gcggattcag tagtggttga aagtggcctt aaatcaggag
     6961 atgtcgtggt ttatgattga cagaccttta atccaacttt ctgatttggt gaagacttat
     7021 catcttgagg gtatcagtac cacggtatta aaagcagtgt ctttgaccgt aaacgaaggt
     7081 gatttgctcg ctattgtcgg tgcgtccggc tctggtaaat ccacgttaat gaacatcatt
     7141 ggcttattag ataaaccgga tgcaggaact tataccttaa ataatagaaa tgtggccagt
     7201 ttaagcgacg atgaggcggc tgatttgcgc aatcaaaata taggttttgt ttttcagcag
     7261 tttaatttgc ttcctcgttt tacagcgatg caaaatgtgg ctttgccttt gacgtaccga
     7321 ggagtcaatc ccactttgat aaaagaaaaa gtggagcaag cattggataa agtaggaatg
     7381 ctccaataca tccgccacag gccaacccaa ttatctggcg gtcagcagca gcgagtcgct
     7441 atcgccagag cgttagtgac cgacccacag gttattctcg ctgatgagcc gaccggtgcg
     7501 ctggattcaa gaacaggtag cgaggtaatg aatttgtttt tggctttaca ccaggaaggc
     7561 cggactatta tcatggtgac ccatgatgag cacgtggcag cgcaatgcaa acgccgaata
     7621 accttagccg atggagcaat tatagcggag tccgggcaat gaattttctt aatcattgtc
     7681 aacaagcctt ggttaattta acagcctcca agctccgatc gcttttggct gtgctgggaa
     7741 tactggtagg gaccgccgct gtcgttgcct tgattagctg tggacagctg gctaccgaga
     7801 aggcgctgga gcaattcaag gcattgggta ctgatttgct ggctgtttct gtttatcaaa
     7861 aggtgcccag caaatcccat agtgaatccg actcgttaac aatagatcaa tggcaacaga
     7921 tagccgagcg tataccttat gtcttaagaa ttgcgcctta cagcacggct taccaaactt
     7981 taagctttaa tggcaaagtg atgcaggggg cggtgattgg cgccgatgaa agccttgctg
     8041 atatcattca tgtgaccttg gcgcaggggc attttgtttc ttttttggat tcttttgagc
     8101 atttttgtgt tattggcgat agcctggcac aacaaatcaa agaaattacc ctggatgatc
     8161 ccattggcaa gcaattacgc ataggccaat ccttgtatac cattatcggc gttgccgagc
     8221 gttggaaaga aaatggtttt tttaatgagg acattaacca ggctgtcatt attcccctgg
     8281 ctggaattag cctggtcagc aaggacgcca aaatcaataa tgcagtattg cttctcaagc
     8341 cggacagccc cattgatgag gtcattaatg aaataaagga aatcattaat tctcaggcgc
     8401 cgaaactcag tgtttttccg cgcagcgcca aacaaattat agccagcatg gaaaatcagg
     8461 gccgtatttt taccttgctg ttggcagtca ttggtggaat atccttgctg gttggcggta
     8521 tcggtgtcat gaatgtgatg ctggtttcgg tcagcgagcg caaaaaagaa attgggattc
     8581 gcaaagcagt gggtgcgaaa aacagagaaa tccaggcctt gtttttggtg gagtctgtca
     8641 tgctgtcttt gctagggggt gtactcggag ttgttttagg attgattttt accagaatag
     8701 tcgcttattt cagtgactgg acttttacta tctacttact ccccccaatt gctggctttc
     8761 tggtttctgc tgccactgga atctttttcg gattttatcc ggcccgacgg gcctcgaaac
     8821 tggagccgat ggtttcttta cgcagtgaat agtttattag ggtctgttga catttctact
     8881 atcttggccg aaaagccttg attttctgtg ctccgttact cacatacttc tatgtatgct
     8941 ccgctacggt gctcgaaaat caaggctttt cgactcaagc gagcgaaatg tcaacagacc
     9001 ctaaagtcct acgattcttt tgtataaaac aggatacccg ctcgctcaat gtgttcaagc
     9061 aaatcgggat tgtctatggt gtgtttcagg gatatatcga ccttccaggg taacagtaaa
     9121 tcatcaattt tattttcaat agcaagtaat tcagtgatcc ctaataagtt tccggtaagg
     9181 caaagatcaa tatctgagcc ctgatgatat gtgcctttgg cacgagatcc gtagagaatc
     9241 gcattatcaa tttgaggata ctctttaaag acacatttca atgcttcaat agcatggctg
     9301 ggcaagccat acagatggtt taattgctca ttcatgctct ttcagacctt gcattttttc
     9361 caggaatgct tgaaaagaag tgtggtagga attaataata tttttgacaa tttcatcggc
     9421 aacggattgg ttgtaggtat gagaggtttg gttacgactt ttaatcattt ccatccagat
     9481 ttcaccttct ttaattaatc ccttgttaaa tgattcgcgt gttgcatcac gagaaccagt
     9541 aattgcagaa tttccctgga aaaagaaata atctttcatc acattccaag ccagctcatg
     9601 agtaaattca aaggtttgga ttaagccttg cttttccaag tcggataagg gtcttgtttg
     9661 cgccaggttg acggcaagtg ataattgttg aagcgcactt gcataattat ttaaccgttg
     9721 ttgccagcga acatcaatat tggtcataac ttacttactc cagattttga ggttgtaccg
     9781 gttaatttaa tgaagcgact ttatcaaatt ataaaaactc attttataat gaaaaacacg
     9841 aggtattcca gaagcgtttt ttatctaaac aggttttttg caccggattt acaagtactt
     9901 taagaccata acctatatcc ctcttgcatg actcacctct ttttgaaagg ttgccaaaag
     9961 aagttggatt ttaccctatt ttactcacat ttctgtgata tgccaaaagc gtttttgtac
    10021 aattccaata acaaatcacc actatgatct cccgctgctt catcaacaaa aggcgaactg
    10081 tttaatgcaa taaccactat tttttcatta caaggatgat aaatatataa gactctaaat
    10141 cccactgtat caccttcata ataccaatat tttcccaatt gcggcttata gccttgaaca
    10201 acacctaaac caaagccatt aggatcttct gaatcaacat gggagattgg agtacccgtt
    10261 ttcatcgaaa tgagggattt taactccatt tgctgtttcg gcagtaaaaa atcggatgaa
    10321 aataaagctt ttatccattt ggcgacatcc gatgtagttg cgacaatagc accagcaggt
    10381 cccgccattg ataaattgtc attggttgcc ttttgaccat gttttaagtt ttccactgtt
    10441 ctaccataat agtaactttc cggcattttt tttaaaaaat gtggggtata agaatcagta
    10501 atataatagg tctcttttaa ttgtaagggc tttagaattt tttcctgtaa ttgattatga
    10561 aaggaatcgt gagtgacttt ttctataatc atacccagaa ttaagtagtt ggtatcagag
    10621 taattataac ctgaggtggg gtgtttatat ccatatgcta aatctaccag ctctttacta
    10681 gtccactgtt ttttaggatt atcagtaatc gaatctgcaa gcgttggaat cgttgcataa
    10741 cctggaattg tacttgtcat attaagtaat tgctttatag taatgccttt ccagtcatcg
    10801 tattctggca accaatctcc aattgtttgg ttgatgttta atttcccttc cgcttcaagc
    10861 tttaagatta atgcagctgt aaacgatttg gttatactgc caatttgaaa atatgcatta
    10921 tttgtaaggc gtttttttct tttattgttc tcaaaaccat gggctgttga tattatttca
    10981 gttccgctta aaaaagtcgc acccacacct gtaaccagct cttcattttt atatttttca
    11041 atgtatttat caattaatga gtcaattgat ttcaatttaa cttgctcgcc cgcaaataag
    11101 ggcgtagata gaattactgc gcccattaaa ctccatgtga atatattttt gatatgcata
    11161 tttaactcct ttggatcaaa gcctcagtta tactatgaac actcatttct ttttattcca
    11221 gtttacttac atacaagcat atcagtacct gggttatggt atttaatctc tgcatgttaa
    11281 agaagctgtg ctccctagag actctttgtt ttttgcaata ctgcatccta aaatctattt
    11341 tgtcatcaaa ggagttatca tattgacaaa aaatagaatg gaaaaagatg cgaaaaaatc
    11401 ccataacaat cttcgcaggc caagactagt ctatagctgc actttcttct aatgagcggg
    11461 ttctaattat agatttccat gaaagattac tttctgtaca atacttattg ttgggtctga
    11521 ctttctggtt caagctttgt cggtatattg ccagagaatg acgaataaac aggccaattt
    11581 ttaataaatg agctatcttt taagaatacc agtatgtttt gagcggattg tttattgtaa
    11641 caattaaaga gaaagaaacg ctgggcgaaa tacatattat taaacaataa atataactga
    11701 ggggaagagc tatgaaaaag tgggggctcg ctattgttgc actgatgtta tttaaccaaa
    11761 taacctatgc aagtcaaagc tggaatcaat gggttgccgg ggtgcgtgag gaagccttac
    11821 aacaagggat tccacccagt ttatttgatg aagcctttgc cggcattcat gaaccaagcc
    11881 ggaaaataaa aggtttttta cgctctcaac ctgaacacag gttaacgttc atgaaatatc
    11941 gtaataccag agcagacaat taccgaattg tgattggaag aaaagagtac aagagaaaca
    12001 aggaaatact ggaagagatt gcccaacaat acggagttaa tccttgcttt attgtgtcgt
    12061 tttggggtat ggaaaccagt tacggctcct acatgggaaa tttcccggta atcaatgcgc
    12121 tggctacttt ggcctatgat tcaaaccgta aagacttttt ccgtaaggaa ttgttcctcg
    12181 ctttacacat tctgaagggc gggcatgtca gcctggctga tttcaagggc gagtgggctg
    12241 gtgcctctgg ccaacctcaa tttttaccat ccagctgggt caaatacgct gtagactacg
    12301 atggggatgg acgaaaagac atatggaaga gcaagcccga cgtttttgcc tccatagcga
    12361 attacatgaa acaaaacggc tggcaagcag gtgagccttg ggctattcag gtgaagcttc
    12421 ctgccaagtt tgatatgaaa ctggaaggta aaagcattgt caaaccagtc agcgaatgga
    12481 atgctttagg tgtgcgcacg gaaagcggtg aacccctgcc ctatcaaaat ttgcaggcca
    12541 gtatcgtaca gccttatggt ggcccgacct tcctggcgtt tcccaactac aagatgatcc
    12601 tgcgttataa taactccatt tattatgccg gggctatcgg gtatatggct gataaaattt
    12661 gccagggtca atgatttgct ggcgacataa atatctactg atgaattcca ctgggacagg
    12721 cgttcagtgg agttctcata ggccaagctt tttaagcttt ataaatcata ggtaacgtga
    12781 gttatggata aaatcagttg ttatctcgag ccgctcgggc tgaggaggca tttatgccgt
    12841 ctcgaagcct agtgtcggat tttatgtttc atgccaaggc ttcgagacga tgcttaggca
    12901 tctcctcagc ccgaacgtac cctgggtatc gacgggctgt tatatcctct ttatccataa
    12961 ctcaccttag gtattaaaat gaagcataaa tcctttcagt ggtcagtggt tggtgcgggg
    13021 cctgccggaa ttgctgcagt aggtaaacta ttggattatg ggattgcacc ttctgaaata
    13081 ttatggattg atcctcattt taaagtgggt gattttggtc agctctggcg gcacgtttcc
    13141 agtaatacta cggtcaagct cttctcaagt tttctgcatg ctgcaaactc attttcctat
    13201 aaaaaagcac cctccacatt tcgtttaaac aacctggatc ctgataatac ctgcctgttg
    13261 agtgacgttg cggagccctt gcaatgggtt accagcgttc tggtgaatca ggttattgca
    13321 gaagagacag tcatccacag cttgtctctt tctggaggtt gctggacatt atgctctgat
    13381 acacaacaat accgagcgaa aaatgttatt ttagcaacag gggctgtccc atccagcctc
    13441 aactaccctg gtgttaatgt ggtaccgttt gaaattgcaa tcgataaaca gcgtcttgcc
    13501 catgttgttg atacgaatga gacttacgga gtttttggct cctcccactc ggccattatg
    13561 attttacgct cgcttatcga attaggcgta aagaaagtga ttaattttta tcgctcccct
    13621 tgccgttatg ccatcaatat gggcaattgg attttattcg acaacacagg cttgaaaggt
    13681 gatacggctg cttgggccag gaaacacatc gacggtgtct tgcctgcgaa tcttgtccgt
    13741 tactacccaa gcgaattaaa tattgcacgc catcttccag aatgcgataa agtgatttac
    13801 gccgttggtt ttgaaagacg taaaaatatg accattgata attatgaaca cgttactcct
    13861 aatcctcatg tgggcattat agggccaggc ttatttggct tgggcatagc ctatcctgaa
    13921 ctcagtgtcg atccgtttgg gactattgag catcaggtgg gattatggaa gttcatggtg
    13981 tatttgtgta aagtgctgcc tgtctggttt aaatatcctg cctagagttt tgagatgata
    14041 actcatgaca tgcccactct tcatcggctg gttttctaca gactcgcaag actctgttat
    14101 attgacatag ttaggaaacg ccaaggataa aaaaatgata aagatattaa tttatttagg
    14161 actgttttta agcagcaatt tactcatggc gaaatcattg tcgcaattgg actctgctaa
    14221 cttggtcccc tgttttaaca tagagcaagc agaacgcatt ggaaaacaaa tcaataagct
    14281 cttacagcat gagttttgcg aggaaaacat caacccaaaa aaatttgcct ctatcagtca
    14341 caatattctg cccaaaatta tgacagaaac atttttagga gtaaccccgc cagaaaactg
    14401 gcagcaatta agcgacgata ttataaaaaa ctgcatcgaa aacaagaatc tatgcaaaaa
    14461 agcagctcgc aaagagttgg aagaatgcat caaaccgaga attcctttga ttctgattca
    14521 attcggcccc tggcttgccc aaaattgtcc acaattgaat aagtccttga ttgaacaatg
    14581 gccaaataaa caggccattc tcaaaaagat aattaatgaa aataaaagtg tcgagtaatc
    14641 gaggcaggct taattacgag agttatgccg gataaaacca catttattta cctaattcac
    14701 ctaacttcat caaatataac ttaccggtat gatctacaac caggttctta aaacgatcgt
    14761 tttatataca tgccaaatac gtcattgact tacttttatt taggatata
//
LOCUS       DBNCWK010000067        14792 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.067, whole genome shotgun sequence.
ACCESSION   DBNCWK010000067 DBNCWK010000000
VERSION     DBNCWK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14792
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.067"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..682
                     /locus_tag="AC06IO_001807"
     CDS             <1..682
                     /locus_tag="AC06IO_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIO9874986.1"
                     /translation="SSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRR
                     LHATCTFEAYIFDDLFPALKKREVDLVIASMIITDERKKHFIFSLPYMESNSQYITTV
                     DSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDMLLGLSNNEVDASL
                     MDYEAAKYWMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTY
                     LKLYSEYF"
     gene            complement(730..2439)
                     /locus_tag="AC06IO_001808"
     CDS             complement(730..2439)
                     /locus_tag="AC06IO_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIO9874987.1"
                     /translation="MDKRWFEQYQNGVPHEIDTSQYLSLISLFNESCNSYASRAAYSN
                     LGTEITYAELDSLSRNFAAYLQQLNLEKGARVAIMLPNVLQYPVAIFGVLRAGYVVVN
                     TNPLYTADEVIHQMNDSGAEVIIVLANFAKTIEKALSSMPSVKHVIVTQVGDLFPSFK
                     RFIVNFVVNHIKKLVPAFTIPHAVAFNYALSEGKQATLHRVELNHDDIAFLQYTGGTT
                     GVAKGAILTHGNLIANVMQAYTWISPLGVSNQDVIVTALPLYHIFSLTANCLTFMKIG
                     AKNILITNPRDMGHFIDQIKNSGFTAITGVNTLFNALLNHPKFKEIDFSKLKLALSGG
                     MALQKSVSLKWSEMTKTRVLEAYGLTETSPAVTINPMYLDGYNGSIGLPLPSTDVSIR
                     DESGKEVPIGTSGELCIKGPQVMPGYWKRPDETALVFTKDGFLKTGDIARMDEEGFIY
                     LVDRKKDMIVVSGFNVYPNEVEQIIGMHPGVLEVGVVGIVDEESGERVKACIVRKDST
                     LTAEQIINHCKEHLTAYKIPKVVEFFDELPKTNVGKILRRALKNQEKEQKSAVNKKTM
                     EPV"
     gene            2511..3206
                     /gene="minC"
                     /locus_tag="AC06IO_001809"
     CDS             2511..3206
                     /gene="minC"
                     /locus_tag="AC06IO_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HIO9874988.1"
                     /translation="MGENITNTQAFKLKGRLYTLTVLQVLNTDKDAFSQQFAEIVVRA
                     PKLFERTPVVFDLSSVNHLEFDLQELCQIARNHGMIPLAIQGGSPLHDTLAQCQGLGI
                     LNASTAHDKPIIEESSECQSIENFKTKLLTTPVRSGQQVVAKGADLIVTSSVSHGAEL
                     LADGNIHVYGTLRGRALAGISGDIDARIFCQSLDAELVSIAGFYRISDAIEPVNGPCQ
                     IYLANEHIVVEPL"
     gene            3203..3931
                     /locus_tag="AC06IO_001810"
     CDS             3203..3931
                     /locus_tag="AC06IO_001810"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIO9874989.1"
                     /translation="MIDSVFISDLHLHPEDNEIQSRFDHFVNWARLSVKRIYILGDFF
                     HAWSGDDSINAWSTAIAKQIESLTQSGIEIYFMTGNRDFLLGSLFAKRTGWKILTEPT
                     IITLGNHKILLVHGDRYCTNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVNGVRKI
                     SLKNHHKSIEQMDIVTEDAIRHMSENNTEILIHGHTHKPGITNYTYEEKILTRYVLSD
                     WDDIPKLLCYDNTKGFYFTQYDIN"
     gene            3928..4674
                     /gene="ravY"
                     /locus_tag="AC06IO_001811"
     CDS             3928..4674
                     /gene="ravY"
                     /locus_tag="AC06IO_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavY"
                     /protein_id="HIO9874990.1"
                     /translation="MRSYPMSKPSNKMKEELEQIRKTEIENAIAKEREDLLAQRNRER
                     AEADDNLKKGRYIKDPRTGQSITLWESAVQKAEEVLNADLNSYMDARAAIMSLLKMYY
                     EMVKANAQTMKELRAGIGNTIMDWGVFPIKDYIGKKLTGNPEIDLPILQHEVSYTDDN
                     KLKIEPLTRSDKREEKGQLDKLFEGMIHMWLKNNDYTPDRDNPGQFVNSHGETLDKAT
                     FDKLKKDDQHGLNHFLTEYDPDLQFRPSRP"
     gene            complement(4763..5386)
                     /locus_tag="AC06IO_001812"
     CDS             complement(4763..5386)
                     /locus_tag="AC06IO_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HIO9874991.1"
                     /translation="MLKRLDSDLQTLNDFLKTKTPEAWLNHAAANIPLLLLDHAHCER
                     KAAGTAINFISKYPEKAELVATMAPLAREELLHFEKVIDIMKQKGIVYSPLQPSDYAS
                     NLHKHVTNKDGIERLCDQLIIGAIIEARSCERFSSIIPYIEDRDLARFYKTLVKSECR
                     HFEEYLHLAKFYGGSVDDRISEFLTIENSFILSDDVVFRFHSGLPPA"
     gene            5460..6344
                     /gene="dotZ"
                     /locus_tag="AC06IO_001813"
     CDS             5460..6344
                     /gene="dotZ"
                     /locus_tag="AC06IO_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 secretion system coupling complex
                     assembly protein DotZ"
                     /protein_id="HIO9874992.1"
                     /translation="MDEIKKDDELSQWLSTYGTITAERILGRYNISLPQDEILEAINI
                     PSSFYRHLLQIPLKNVLNGIVIQQASDYHVYAQKLLIDYLLSGESSKEPDSQGAGTRE
                     SLEDERQRLVQLGDEFHKLELEQDNLIASSQASLMKISIDWNTKLETTLSKLNSLYKN
                     TNSKIKKNAIRKALIKAFIHCDLVKDQSQKNKYQLIDKLNQTLAVSVGAELKESILTN
                     LSELFQILDALNTKLDEFTVRTNHLSQQAKSFRTQFYEVILRIIELIKLLPEYKIDPE
                     QDAINREPLYFDRTIGER"
     gene            6479..6904
                     /gene="ndk"
                     /locus_tag="AC06IO_001814"
     CDS             6479..6904
                     /gene="ndk"
                     /locus_tag="AC06IO_001814"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIO9874993.1"
                     /translation="MAKELTLSIIKPDAVAKSVIGEIYTRFEKAGLDIVAAKMTQLSR
                     EQAESFYDIHRARPFFKDLVDFMISGPVMIQVLKGENAVAKNREIMGATNPKEAAPGT
                     IRADFADSIDANAVHGSDSLENAAREIAFFFEPHELCNR"
     gene            6925..8073
                     /locus_tag="AC06IO_001815"
     CDS             6925..8073
                     /locus_tag="AC06IO_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HIO9874994.1"
                     /translation="MDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGIRD
                     FAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEAN
                     RGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHDKKIT
                     NVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVA
                     LAVSLHAPTDELRNELVPINKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQP
                     EHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDKLMKHGINTITRKTR
                     GDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTDKSTELTISSEEIA"
     gene            8253..9035
                     /gene="pilW"
                     /locus_tag="AC06IO_001816"
     CDS             8253..9035
                     /gene="pilW"
                     /locus_tag="AC06IO_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HIO9874995.1"
                     /translation="MLKSIRYLLTIIICLFVGACQHTQDNEEPNTIRKVNLSKAASFN
                     VQLGLGYLKQGDRPRAKKKLLTALEQQPDSADVNAALAYYFEQTKELEQAKKFYHKAI
                     TLSQNGGAQLNNYGAFLCRQGDYKNAETYFLKAVKDQNYVHTSGAYENAGLCAMAIPD
                     HDKAILYFTKALNQDPSRKESLYELVKLQSKIGHDKEALELLQKHADLVLNDKVMLAL
                     AKDIANKTGQYTLAAEYENSFNKLEQTNNISGVNDEYNSSNG"
     gene            9010..9579
                     /locus_tag="AC06IO_001817"
     CDS             9010..9579
                     /locus_tag="AC06IO_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9874996.1"
                     /translation="MNTIAAMDETRNNEFTNPGYELAALRQQKGYSIEYVASKLHLRA
                     RIIELIEAGDFDLLPQPVFVKGYLRAYSKLLGVSPEPFLTVFNAQYCFEKKPERAALW
                     QSKKESHKAEHFIKWFTILFAIGVLVAVGIWWQKNKDSQSIDTPKENSSGLSISETSS
                     EIKLTDLSKMESLLTPNNTQMTPLEKNGG"
     gene            9569..10849
                     /gene="hisS"
                     /locus_tag="AC06IO_001818"
     CDS             9569..10849
                     /gene="hisS"
                     /locus_tag="AC06IO_001818"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIO9874997.1"
                     /translation="MVVDKIQAIRGMNDILPDSTSVWRFIEQTFINCLVRYGYKEIRF
                     PIVENTQLFKRTIGEITDIVEKEMYTFNDLNGDSITLRPEGTAGCVRACIEHGLLHNQ
                     QQKLWYLGPMFRHERPQKGRYRQFNQFGVEALGITGTAIELELISICRRLWTDLGFSQ
                     SVQLQVNSLGEINERQKYRSILVEYLRDHFQILDEDSKRRLDKNPLRVLDSKNPDLQQ
                     LIQNAPKLIDVLGDDSREHFQSFCNGLETLGIPYSINPVLVRGLDYYGQTVFEWVTDQ
                     LGSQATICAGGRYDMLVEFLGGAPTPAVGFALGLERIFLLMETLNLLNESNNKQSIFI
                     IATNEEAILKALVMAESIRNAHPSLDVITNTAGGGFKSQFKKADKSGARLALILGEDE
                     IAREYVSIKDLRTEIEQISIPMTKINEFLQDYLA"
     gene            10867..11541
                     /locus_tag="AC06IO_001819"
     CDS             10867..11541
                     /locus_tag="AC06IO_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIO9874998.1"
                     /translation="MSVYMTEEEQLEIIKKWWKRYGNMITIFLSIVLLIIAGYRYMNW
                     HNDKIKQQASIAYENMMIAFSNQNIKSVRSYANELIKNYNHTVYADIAHMTLAKIYVS
                     KNKLEQARNELKAVAAESQMTPLRQIAKIRIARLLAADKAYTNALNELSAVEDEAYLP
                     VINELKGDIYSAKGQYQDAMNAYRLAIDEVRTNGMGNLFLEMKTNELAIKTQSMITDD
                     KKVKAA"
     gene            11567..12721
                     /gene="bamB"
                     /locus_tag="AC06IO_001820"
     CDS             11567..12721
                     /gene="bamB"
                     /locus_tag="AC06IO_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIO9874999.1"
                     /translation="MKIRILVLILCALTQGCTYVDDYMLGKDNTPQPKELKEIQPKVK
                     MAQSWTTPVGKAHKTNEYLNIKPAIRGDIIYTADASGLVQAVNRKDGQIKWSTALKNN
                     IVSGPTVAAGYVAVGTNASTLVLLNQSDGKEIWQNKVSAEVLAPPAISHQKIIAKTID
                     GKVYAIDAVNGKQLWVADHGAPSLVLKASSSPIIVDDLVLVGFSDGKLDALELQTGRL
                     IWQRSIAYGTGASDVERLVDIDSDPIISNNVAYLATYQGYVGALSLSNGQFIWRKPAS
                     VYKNMLLSHNNLYFTDSNDVLWSLNSSTGQVNWKQTSLKARGLTAPALVGGNLAVGDK
                     TGYLHILSTQTGELLGRSQLSGGVTVSPSVSGKNMYVLTNNGMLNQLSVS"
     gene            12726..14114
                     /gene="der"
                     /locus_tag="AC06IO_001821"
     CDS             12726..14114
                     /gene="der"
                     /locus_tag="AC06IO_001821"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIO9875000.1"
                     /translation="MIPVIALVGRPNVGKSTLFNRITKTQDALVADFPGLTRDRQYGH
                     AQHENKSFIIVDTGGIGVDDIEVDTLMSRQSQVALNEANVILFLVDGRSGLTGIDQQI
                     AQALRKLNKKVHLVVNKTDGINEDIACADFQSLGITDIHAISASHGGGISSLLEEILE
                     PFITEMHEATDDKAIKIAFAGRPNVGKSTLINRILGEERVVVYDMPGTTRDSISIPFT
                     REDKQYVLIDTAGVRRKSRIDEKIEKFSVIKTLQAIKEAHVCLLLLDANEGITDQDMN
                     LLGFIIESGKALVIAVNKWDGLEEEHKEKIKSELSRRLHFANFAKIRFISALHGSGVG
                     GLFKDINEAYHSAIQSFSTPKLTRLLQDISAKHTPPCINGRRIKLRYAHLGGHNPPVI
                     VIHGNQLDALPESYKRYLNNEFIKHLGLVGTPLKIEFKGGQNPFANKKNKLSQRQVNK
                     KKRLMRWAKSKK"
     gene            complement(14383..>14792)
                     /locus_tag="AC06IO_001822"
     CDS             complement(14383..>14792)
                     /locus_tag="AC06IO_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIO9875001.1"
                     /translation="LFATDLLNEHSHLTEKAANIAKQFNAKLYLLHVIELPTSILIAQ
                     GLGFTELANPSKEDAQTVLSLIGESLQIPQEQQFVEIGSVKEHILNKAKDLNCQLIII
                     GSHSATGLPSILGSTAHAVVNHSVCDVLTLKAD"
BASE COUNT         4992 a         2585 c         2848 g         4367 t
ORIGIN      
        1 ttcctctaat tcttattcat taaatttaac aattggtact tcaaaattta atccgccttt
       61 tgaagtttgg tctggtaaca acagttcatt gtatggcttt gatattgacc ttatgcaaga
      121 aatatgtaga agattacatg caacctgtac ttttgaagcc tatatattcg atgatttatt
      181 tcccgcgtta aaaaaacgcg aagtcgatct ggttattgcc tcaatgatta ttacagatga
      241 aagaaagaaa cactttattt ttagtcttcc ctatatggaa agtaactcac aatatataac
      301 taccgttgat tctaaaatca gcacatttga tgatttgcac ggaaaaaaaa ttggagtaag
      361 aaaaggaact ccttacgcaa ggcaagtttt gtcagaaaac cgcaacaatc aggtaatttt
      421 ttatgagctc atacaagata tgcttcttgg cttaagcaat aatgaagttg atgccagttt
      481 aatggattat gaagcagcaa aatattggat ggcatctgag ccctatgctt ataaattaat
      541 tggtaagaaa atttctattg gagaagggta ttctattatg gcaaaccctg atcaatttgt
      601 attaattaaa aaaataaata aaattcttct tgaaatggaa gctgatggaa cttatctgaa
      661 actatattca gagtactttt agaagaggag tattaaggac aaatagcacc caaaatactc
      721 caattatacc taaacaggct ccattgtttt tttattgacc gcgctttttt gttccttttc
      781 ttggtttttt aaagcacgac gtaaaatctt gcccacattt gttttaggaa gttcgtcaaa
      841 gaattcgaca actttgggga ttttataagc ggtcaaatgc tccttgcaat gattgataat
      901 ttgttcagcc gtcaatgtac tatcttttct tacaatacag gctttaaccc gttcaccaga
      961 ttcttcatca acaataccta caacgcctac ttctaataca ccgggatgca ttcctattat
     1021 ttgctctacc tcatttggat aaacgttgaa tcctgaaaca acaatcatgt ctttttttct
     1081 atcgaccagg tagataaagc cttcttcatc catacgtgca atatcgccag tttttaagaa
     1141 cccgtcttta gtaaatacca aggctgtctc atcaggcctt ttccaataac caggcatcac
     1201 ttgcggtcct ttaatgcaca actccccact tgtaccaata ggcacttctt tcccggattc
     1261 atcacgaata gatacatcag ttgagggaag aggtaagcca atactgccat tatagccgtc
     1321 aaggtacatc gggtttatag ttacagcagg gctagtttcg gttaatccat aagcttccaa
     1381 aacacgtgtt ttagtcattt cagaccattt taaggacaca ctcttttgca atgccatacc
     1441 gcctgataac gccagtttta atttgctgaa atcaatttct ttaaatttag gatggttcag
     1501 caatgcattg aataaagtat taactccagt tattgctgta aaacccgaat ttttaatttg
     1561 atctataaag tgtcccatat cacgtggatt ggtgatcaga atatttttcg cgccaatttt
     1621 cataaaagtg aggcaattgg ccgtcaagga aaaaatatgg tacaaaggaa gtgcagttac
     1681 tataacatct tgattactga ctcccagagg agatatccag gtgtatgcct gcatcacatt
     1741 ggctataaga ttgccatgag tcaaaatagc gccttttgca acaccagtag taccgccagt
     1801 atattgtaga aaagcaatat catcatgatt cagttcaact cgatggagcg ttgcctgttt
     1861 cccttcagat aaagcatagt taaaagccac ggcgtgagga attgtgaagg caggaactaa
     1921 tttttttata tgattcacta caaaattaac aatgaatctc ttgaatgaag gaaataaatc
     1981 gcccacttgc gtaacaatca catgcttaac ggaaggcatt gacgacaatg ctttttcaat
     2041 cgtttttgca aaattagcca gcacgataat gacctctgcc cccgagtcat tcatttggtg
     2101 tattacttca tccgctgtgt ataaagggtt ggtgtttacc acgacataac ctgcacgtaa
     2161 cacaccaaag atggctacag gatattgtaa aacgttaggc agcattattg ctactcttgc
     2221 ccctttttcc aaattcaatt gctgcagata agcagcgaaa tttctgctta aactgtccag
     2281 ttcagcataa gtaatttccg tacctaaatt ggaataggca gctctgctag cataagagtt
     2341 acatgattca ttaaataaag atatgaggga taaatattga cttgtgtcaa tttcatgtgg
     2401 tactccattt tggtattgtt caaaccatcg tttatccact ttgatatcct tctgttattg
     2461 ttttcctcat gatagtataa acaaaaaact ctctaatgga tatcatgcta atgggtgaaa
     2521 atataactaa tacacaagca tttaagctta agggaaggtt gtatacttta accgttttgc
     2581 aagttttgaa cactgataaa gatgcttttt ctcaacaatt tgctgaaatt gttgttagag
     2641 cgcctaaatt atttgaaaga acacccgttg tgtttgactt gtcttctgtc aatcatctcg
     2701 aatttgattt acaggaatta tgtcaaattg cacgtaatca tggtatgatc cctttggcaa
     2761 ttcaaggtgg tagtccttta catgatacgc tggctcaatg ccagggtttg ggcatactca
     2821 atgcttcaac agcacatgat aagccaataa tcgaagaatc ctcagaatgt caaagtatag
     2881 agaatttcaa aacaaaatta ctaacgacac cagtgcgttc aggccaacaa gtcgttgcca
     2941 agggagctga tttgatagtg acttcttcag ttagccatgg ggcagaatta ttagctgatg
     3001 gaaatattca tgtgtatgga acattacgcg gcagagcatt agcaggaata tccggggata
     3061 tcgatgcaag aattttttgc cagtctttgg atgctgaatt agtatctata gcgggtttct
     3121 atcgaataag tgatgctatt gaaccagtta atgggccttg tcagatctac ttggcaaatg
     3181 aacatatcgt tgttgaacca ttatgattga ctccgttttt atatccgatt tacatctgca
     3241 tccagaagat aatgaaattc aaagcagatt tgatcatttt gtaaattggg ctcggctatc
     3301 tgtaaaaagg atatacatac taggtgattt ttttcatgcc tggtcaggtg atgattcaat
     3361 taacgcatgg agtactgcta ttgcaaaaca aatagaatca ttgactcaaa gcggcattga
     3421 aatttatttt atgactggaa atcgagattt tttacttggc tcattatttg ccaagcgaac
     3481 aggttggaaa atattaacag agccaaccat cattacacta ggaaatcaca agatcttgct
     3541 ggttcatggc gatcgttatt gtactaatga cagatcacac caacgattcc gattattaac
     3601 gagaaacagg tggttctcat ttcttttttt aagacttcct ttatcatttc gcaaacgatt
     3661 ggttaacgga gttcgcaaga ttagcctaaa aaatcaccat aaatcaatcg aacagatgga
     3721 tattgttaca gaggatgcaa ttcgacatat gtctgaaaat aatacagaaa tactgattca
     3781 tggacacacg cataaacctg gcataacaaa ttacacttat gaagaaaaaa tactgacaag
     3841 atatgttcta agtgattggg atgacatacc caaactattg tgttatgata atacaaaagg
     3901 attctatttc acccaatatg atataaattg aggagttacc caatgtctaa accatcaaat
     3961 aaaatgaaag aggaactcga acaaattcga aaaactgaaa tagaaaatgc tatagcaaaa
     4021 gaacgagaag atttacttgc ccaaagaaac cgagaacgcg ctgaggccga tgacaaccta
     4081 aaaaaaggcc gatatatcaa agatcctcgt acaggccaat caataactct ctgggaaagt
     4141 gcagtgcaaa aagcagagga agttttgaat gctgatttaa attcttatat ggatgctcgt
     4201 gctgcgatta tgagtttatt aaaaatgtat tatgaaatgg tcaaggcaaa tgctcagact
     4261 atgaaggaat tgcgcgcagg aattggtaac acgattatgg attggggtgt ctttcctatt
     4321 aaagattata ttgggaaaaa actcacggga aatcctgaaa ttgatttacc aattttgcaa
     4381 catgaagtga gttacactga tgacaataag ttgaaaattg aacctctgac tcgatcagac
     4441 aaacgtgagg aaaaggggca acttgataaa ttatttgagg gtatgatcca catgtggcta
     4501 aagaataatg attatacccc tgaccgtgat aatcctggtc aatttgttaa ttcacacggt
     4561 gaaaccttag acaaagcaac gtttgataaa ttaaaaaaag acgatcagca tggacttaat
     4621 cactttttaa cggaatatga tcctgatttg caattcagac caagcagacc ttgattaagc
     4681 aagagaaata ttgggttaca ctagcctgta tataatactt atgggctagt gctgaaaaat
     4741 ctgttgactc tgttttaagg catcaagccg gaggcaagcc actatgaaag cgaaatacca
     4801 catcgtctga taagataaac gaattttcta tagttaaaaa ctccgaaata cgatcatcaa
     4861 cagaaccgcc atagaacttg gccaaatgca aatactcttc aaaatgcctg cattctgatt
     4921 taaccaatgt tttataaaac ctggctaagt cacgatcttc aatatatgga attattgagc
     4981 taaaacgctc gcacgatcga gcctcaatga tagcacctat aatcaattga tcgcatagtc
     5041 tctcaattcc atctttattt gtgacatgct tatgcaaatt ggatgcatag tctgatggct
     5101 gcaagggaga ataaacaatt cccttttgct tcatgatatc aatgactttt tcaaagtgca
     5161 gtaattcttc tcttgccaat ggcgccatag ttgctactaa ttctgctttt tctggatact
     5221 tgcttatgaa attaattgct gttccagctg ctttacgttc acaatgagca tgatcgagca
     5281 ataaaagagg tatattggca gctgcatggt ttaaccaggc ttccggagtt ttagttttta
     5341 aaaaatcatt taacgtttgt aaatcactat ctaatcgttt taacatgata aaatacacta
     5401 taatttcaaa taaatagccc ttatatcaca tactttaaag aatttaaatt ggataaaaaa
     5461 tggacgagat caaaaaagat gatgaattga gtcaatggct ttctacttat ggaacaatca
     5521 ccgctgaacg tatattaggt agatacaaca tatcactgcc acaagatgaa atactagaag
     5581 cgattaacat tccatctagt ttctataggc atttgttgca gattccctta aaaaacgtac
     5641 ttaatggaat tgtaattcaa caggcaagtg attaccatgt ctatgcacag aaactgttaa
     5701 ttgattattt gttatctggt gaaagcagca aagagcctga ctcacagggg gccggaactc
     5761 gcgaatcact tgaagatgaa agacagagat tagttcagtt aggggatgag tttcataaat
     5821 tggagttaga gcaggataat ctcattgcat caagccaagc cagcttaatg aaaataagca
     5881 ttgactggaa tacaaaactg gaaacaactc tttctaaatt aaatagctta tacaaaaata
     5941 caaattctaa aattaaaaaa aatgcaatta gaaaagcgtt aatcaaggcc tttattcatt
     6001 gtgatctagt caaggatcaa tcacaaaaaa acaagtacca gctaattgat aaattgaatc
     6061 aaacattagc tgtttctgta ggtgctgaat taaaagagtc tatcctgacc aatctgagtg
     6121 aactcttcca aattctagac gctttgaata caaagcttga tgaatttact gttcggacta
     6181 atcatctaag ccaacaggct aaatcattca gaacccaatt ctatgaggta atacttcgca
     6241 tcattgagtt aattaaacta ttacccgaat ataaaattga ccctgaacaa gatgcgatta
     6301 accgagaacc attatacttt gatagaacga ttggagagcg ctaacgcaaa cccctatttt
     6361 ctctaaaatc atactataac tattgactga atggttgaat gcatgaattt ttattagcca
     6421 gaccactatg aattcatgta taataatgca ctttaaatta atctcattgg agtgcatgat
     6481 ggcaaaagaa ttaacattat ctattattaa acctgatgct gtagcaaagt ctgtgatagg
     6541 tgaaatatat acccgctttg aaaaagcagg cttggatatt gttgccgcta aaatgacaca
     6601 actttcacgc gaacaagccg agagctttta tgatattcat cgcgcgcgac cattctttaa
     6661 ggatctggtg gattttatga tttctggtcc tgtaatgatc caagtattaa aaggtgaaaa
     6721 tgctgtcgct aaaaacagag aaattatggg cgcgacaaat cccaaagaag cagcacctgg
     6781 aactatacgt gctgattttg ctgatagtat tgatgcaaat gctgtgcatg gttctgacag
     6841 ccttgaaaat gctgctcgtg aaatagcatt tttctttgaa ccgcatgagt tgtgcaatag
     6901 ataaaggcag gcttgggagc ggtaatggac caacaaaaag tgaatctatt aaattataat
     6961 tactcacagc tacgagaatt attgatagcg tgggatgaaa aacctttcag agcccaacag
     7021 ctatttcaat ggattcatca agttggcata cgtgactttg ctcagatgac taatttaggg
     7081 aaagttttaa gaaataaact ttcccaatta gcttgtatag atttacctga aatcgttgca
     7141 tgccaaaaat cagctgatgg cacacataag tggctattaa agctggaatg tgggaattgt
     7201 attgaaacag tgttcattcc agaagcaaat cgagggacac tttgtgtttc atcacaagta
     7261 ggttgtgctt taaattgctc tttttgttcc acagcaaagc aaggatttaa cagaaattta
     7321 tcaacagctg aaataattgg tcaagtatgg ctggcagcac gcgaactatc agataataat
     7381 ggcactcacg ataaaaaaat aactaacgtc gtgatgatgg gaatgggtga accgttactg
     7441 aatttcgata atgttgtttc tgctatgaac atcatgatgg atgatttagc ctatggacta
     7501 tccaaaagac gggttacatt aagtacttcg ggtgttttac cggaaatgga acgactcaga
     7561 gaagtcagtc ctgtcgcgtt ggcagtatcc ttacatgcac caacagatga actacgtaac
     7621 gagctagtac caattaataa aaaatatcct ctctctcaat taatatcact atgtaaacga
     7681 tattttaaag acgaaccaag aagaaaagtt acttttgaat acgtcatgct aaaaggggtt
     7741 aatgatcaac ctgaacatgc tagtcaatta attaaacttt tgcataatgt gcctgcaaaa
     7801 gttaatttaa ttccttttaa cccatttcct ttaactcaat atcagagatc atcccgggaa
     7861 acaattgatg cttttcgaga taaattaatg aaacatggta ttaatactat tactcgcaaa
     7921 actcgtggag atgatattga tgcagcatgc ggccaacttg ctggcgaggt aaaagataag
     7981 acgagcagat cgcaacgatg gcaaaaactt catttcatga gcaaaacaga caaatcaact
     8041 gaattgacca tttcttcgga agaaattgca taacacagtc ttgtataata accgagagcc
     8101 aattaagaac tttctccagt attcctggtt ggggattatg tataattaag gcgttttcac
     8161 ctgcagactc acattaaata gtgacaaatc atttttaatt tggacttaag tggttataat
     8221 gcgaaatcat ttctaacgta aagtgattaa tcgtgctaaa aagcataagg tacttattaa
     8281 ctataataat ttgtttattt gttggagctt gtcagcatac acaagataac gaagaaccta
     8341 ataccattag aaaagtaaac cttagcaaag cggcttcttt taatgtgcaa ttggggttgg
     8401 gatatttgaa gcaaggcgat agacctagag caaaaaagaa gctgctaacc gccttggaac
     8461 aacaaccaga ttcagcagat gttaatgcag cattagccta ttattttgaa caaaccaaag
     8521 aattggaaca agcgaaaaaa ttctatcata aagcaataac cctatcgcaa aatggtggtg
     8581 cacaattgaa taattacggc gcttttctgt gtcgacaggg ggattataaa aatgctgaaa
     8641 cttacttcct gaaggccgtt aaagatcaaa actatgtgca tacctcaggc gcttatgaaa
     8701 atgctgggtt atgtgctatg gctatacctg atcatgataa agccatttta tattttacga
     8761 aggccttaaa tcaggatcca tcgagaaaag aatcactata tgaattagta aaattacaaa
     8821 gtaaaatagg acatgataaa gaagccctcg aactgcttca aaaacatgct gatttagtat
     8881 taaatgataa ggtaatgctt gcattagcga aagacatagc aaataaaaca ggccaatata
     8941 ctcttgcagc agaatatgaa aatagcttca ataaactgga gcaaactaat aatattagtg
     9001 gagtaaacga tgaatacaat agcagcaatg gatgagacaa gaaataatga atttaccaat
     9061 cccggctacg agctggcagc tcttcgccag caaaaagggt attcaatcga gtatgttgcg
     9121 agcaaacttc atctacgagc tcgtattata gagctcattg aagcaggaga ttttgattta
     9181 ttgcctcagc cagtttttgt aaaagggtac ttgcgcgcct attcaaaatt attaggagtt
     9241 tcacctgaac cttttttaac tgtttttaat gcacaatatt gttttgaaaa aaaaccggaa
     9301 agagcagccc tatggcaaag taaaaaggag tctcataaag cagaacattt tataaaatgg
     9361 ttcaccattt tatttgcgat tggagtttta gtcgcagttg ggatatggtg gcaaaaaaac
     9421 aaggatagcc agtctataga taccccaaaa gagaattcat ctggattatc aatcagtgaa
     9481 accagttcgg aaattaaatt aactgattta tcaaaaatgg aatcgttatt aacgcccaat
     9541 aatacacaaa tgacgccttt ggagaaaaat ggtggttgat aaaattcaag ctatccgagg
     9601 aatgaatgat atactgcctg atagtacctc ggtatggcgc tttatagaac agacttttat
     9661 aaattgcctt gtacgatatg gatacaagga aattcgtttt ccaattgttg agaatacaca
     9721 gctatttaaa cgtacaattg gtgaaataac agatattgtt gaaaaggaaa tgtacacttt
     9781 caacgatttg aatggtgata gcataaccct gaggccagaa ggaacagccg gctgtgttcg
     9841 tgcctgtata gaacatggtt tattgcataa tcaacaacaa aaactatggt acttaggtcc
     9901 tatgtttcgc catgaacgtc ctcagaaggg tagataccgt caatttaacc aatttggggt
     9961 tgaggcgcta ggaattactg gcactgctat tgagcttgag ctgatctcaa tttgccgcag
    10021 attatggacc gacctgggtt tttctcagtc agttcaatta caagttaatt cattaggtga
    10081 aataaatgaa cgtcagaaat atcgctctat cctggtagag tatctacgcg atcattttca
    10141 gatacttgat gaagacagta agaggcgatt ggataaaaac cctttgagag ttcttgatag
    10201 taaaaaccct gatcttcaac agctcattca aaatgcgcct aagttaattg atgttcttgg
    10261 tgatgatagc agagaacact ttcaatcgtt ctgcaacgga ttggaaacac ttggtattcc
    10321 ttattccatc aaccctgtat tggtaagagg attagattat tatggccaaa cggtgtttga
    10381 atgggtaaca gaccaattag gaagtcaagc tacaatatgt gcaggaggta gatatgatat
    10441 gttggtggag ttccttggcg gagcacccac acctgcagtt gggtttgctt taggattgga
    10501 aagaatattt cttttaatgg aaactttaaa cttgttgaat gaaagtaata ataaacaatc
    10561 aatatttatc attgctacta atgaagaggc catattgaaa gcgcttgtca tggcagagtc
    10621 aattcgaaat gctcatccat ctcttgatgt cattaccaat acggcaggcg gtggttttaa
    10681 aagccaattt aaaaaggccg ataaaagcgg ggctcgcttg gctttgatac ttggggaaga
    10741 cgaaatagcc agggaatatg taagcattaa agatttgaga actgaaatag agcaaatatc
    10801 aataccgatg accaaaataa atgagttttt gcaagattat ctagcttaag ggcaggagaa
    10861 tattatatgt ctgtttacat gacggaagaa gaacaattag agattataaa aaaatggtgg
    10921 aagcgatatg gcaatatgat aactattttt ttatcaatag tgctcctgat tatcgcaggg
    10981 taccgatata tgaattggca caatgacaag ataaaacaac aagcatctat tgcctacgaa
    11041 aatatgatga ttgcattttc caatcaaaac ataaagtctg ttcgttccta tgccaatgaa
    11101 ttaattaaga attataacca tactgtttat gctgatatag ctcatatgac acttgcaaaa
    11161 atctacgtaa gtaaaaataa acttgagcaa gcaagaaatg aattaaaggc tgttgcagcg
    11221 gaaagccaaa tgacaccgct aaggcaaatt gccaaaatcc gcattgcacg tttgctggcg
    11281 gcagataaag cttataccaa tgcattaaat gaattaagtg ctgttgagga cgaagcctac
    11341 ttgccagtaa ttaacgaact gaaaggggac atttatagcg ccaaaggcca atatcaggac
    11401 gcaatgaatg cctaccgact tgcaatagat gaagtaagaa caaatggaat gggtaattta
    11461 tttttagaaa tgaaaacaaa tgagttagca attaagactc aatctatgat tactgatgat
    11521 aaaaaggtaa aagcagctta attttattat aaaggtttgt ttttttatga agattagaat
    11581 attagtttta atattatgcg ccttaactca aggatgcaca tatgtggatg attatatgct
    11641 cggtaaggat aatacgcccc aacccaaaga gctaaaagaa attcagccca aggtcaaaat
    11701 ggcacaaagc tggacaactc cagttggaaa ggctcataaa acaaatgagt atttaaatat
    11761 caaaccagct attcgtggcg atataattta tactgcagat gcaagtggat tggttcaggc
    11821 agttaacagg aaagatggtc agataaagtg gtctaccgca ttaaaaaata atatagttag
    11881 tggaccaact gtagctgctg gttatgttgc tgtaggaact aatgcctcca cattggtact
    11941 acttaatcaa tcggacggaa aagagatatg gcagaataaa gtatctgctg aagtattagc
    12001 tcctcctgct atctcacatc agaagatcat tgcaaaaacc attgatggta aagtgtatgc
    12061 gattgatgcg gtaaacggca aacaactgtg ggttgcagat catggcgctc ccagcctggt
    12121 gttaaaagcc agttcgtctc ctatcattgt cgacgatctg gttcttgtcg gattttccga
    12181 tggcaaatta gatgctttgg aattacagac cggacgattg atctggcaaa gaagcattgc
    12241 ttatggaaca ggagctagtg atgtcgaacg tttagttgat attgattctg accctataat
    12301 aagcaataac gtcgcctatt tggcaactta tcagggatat gttggagcac tatcactgtc
    12361 taatggccaa ttcatatgga gaaagccagc atctgtttat aaaaatatgc ttttaagtca
    12421 taataatctg tattttacgg acagcaatga tgtcttgtgg tctttgaaca gtagcacagg
    12481 acaggtcaac tggaagcaaa cctccctaaa agcaagaggg cttaccgcac ctgcattagt
    12541 tggaggaaat ttagctgttg gtgataaaac aggatattta catatcctat caactcaaac
    12601 aggtgagtta cttggacgtt ctcagctttc tggtggagta acagtatcgc caagtgtttc
    12661 tggtaaaaat atgtatgtat taaccaataa tggaatgctc aatcaacttt ctgtgagcta
    12721 ataaaatgat ccctgtcatc gctttagttg ggcgtcccaa tgttggaaag tctactttgt
    12781 ttaatagaat tactaaaacg caggatgctc ttgtcgcaga ttttcctggc ttgacgaggg
    12841 atagacaata tggacacgct caacatgaaa ataagtcctt tattattgtt gatactggag
    12901 gcataggagt tgatgacatt gaagtagata ctttgatgtc aagacagtct caagttgctt
    12961 taaatgaagc aaatgtgatt cttttcctgg tcgatggccg ttcgggacta acaggtatag
    13021 atcagcaaat tgcacaagca ttaagaaaac ttaataaaaa agtacatctt gttgtcaata
    13081 aaacagatgg aataaatgaa gatattgctt gtgccgattt tcaatcatta ggcattactg
    13141 atatccatgc tatatctgca tctcatggtg gaggtatcag ttcactactc gaagaaattc
    13201 tcgagccatt tataactgaa atgcatgaag caactgatga taaagcgatc aaaattgctt
    13261 ttgcggggcg ccctaatgtt ggaaaatcaa ccttgataaa caggatatta ggggaagaaa
    13321 gagtagtagt ttatgacatg cccggaacaa ctcgagatag tatttcaata ccctttacaa
    13381 gagaagataa gcaatatgtg cttattgata ctgctggtgt gcggcgtaag tctcgaattg
    13441 atgaaaaaat agaaaagttt tctgtcatca aaactctgca agctataaaa gaagctcatg
    13501 tctgtctgtt acttcttgat gcaaatgaag gcattaccga tcaagatatg aatttgcttg
    13561 gttttattat tgaatctggt aaagcactag ttattgctgt gaataaatgg gacggcctgg
    13621 aagaagaaca taaagaaaaa ataaagtcag aactgtcaag aagattgcat tttgcaaatt
    13681 ttgcaaaaat cagatttatt tcagcattgc atgggagcgg ggtaggtgga ttatttaaag
    13741 atattaatga agcctatcat tcagcaatac aatccttttc tactccaaaa ctaaccagat
    13801 tattacaaga tatcagcgca aagcatacac caccatgcat caatggccga cgaataaaat
    13861 tacgctatgc tcaccttggc ggacacaatc ctccagtcat tgttattcat ggaaatcaac
    13921 tcgatgcctt gccagaaagt tataaacgtt atttaaacaa tgagtttatt aagcatttag
    13981 gattagtagg tactccttta aaaattgagt ttaaaggagg tcaaaatcca tttgccaata
    14041 aaaaaaataa attatcacaa aggcaggtga ataagaagaa acgattaatg cgatgggcaa
    14101 aaagcaagaa atagcacccc ggttcacctc agccaggttt aacttgttct aatgaaaaaa
    14161 ggtatcaatt ggataataat ctcttcttat taagtaactg ttcatggggt atttaattaa
    14221 ggaatattag gtcttctgtc tttcccgcga aggcgggaat ctatccataa agctagcaca
    14281 aggattgatt tttagttagc ttcccgcctt ctcgggaaag acaactcaaa taaataggct
    14341 tataaacaga aacatttgca taccccgtgt acggctatct tattaatctg ctttaagtgt
    14401 taatacatca caaaccgaat gatttactac agcatgcgct gtacttccta gaatagacgg
    14461 taatcctgta gcagagtgtg agcctattat aattaattgg caattcagat cttttgcttt
    14521 gtttaatata tgttccttta cggacccgat ttctacaaat tgttgttcct gaggtatttg
    14581 taaactttct ccaattaatg ataatacagt ttgggcatcc tcttttgatg ggttagctag
    14641 ctccgtaaaa cccaatcctt gagctattaa tatacttgtt ggcaactcaa tcacatgtaa
    14701 tagatacaat tttgcattaa actgtttagc aatattagca gccttttctg taagatggct
    14761 atgctcattt aacaaatctg tggcaaataa aa
//
LOCUS       DBNCWK010000068        14717 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.068, whole genome shotgun sequence.
ACCESSION   DBNCWK010000068 DBNCWK010000000
VERSION     DBNCWK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14717
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.068"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1062
                     /gene="ceg23"
                     /locus_tag="AC06IO_001823"
     CDS             <1..1062
                     /gene="ceg23"
                     /locus_tag="AC06IO_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg23"
                     /protein_id="HIO9875002.1"
                     /translation="VSEPSGYLFEKSLILGFLLREWFPTQLVNNSAVKAEMLEGEKGV
                     FSAFKNYKEYRSFMSKEELKSTEFGALYEANEAFLEYFYNRSESTLINKDSPFEKYFV
                     GSSSDEEAIKNYWDAEGYTLYCQHLAKPQVKLSYIEIMTMMKVINQPLTIYDRSTSSV
                     VAEYVNPKVNLPDFEVAIDALQGHYFLLKTENTEKELEEYERSYAQYKRDRSEILAHS
                     DKPVSSLLVRATCPKGHLDEDPFIALIESLSEINSLSQIDTNLKNENTDISNCNFLLK
                     VGASVVGTSSALITLMGIAILSGQYDPGNSLAEEALATTVVSSIVGLGYGLYSFFSSS
                     PKVSERDSTNGLSQKLKMN"
     gene            1254..1673
                     /locus_tag="AC06IO_001824"
     CDS             1254..1673
                     /locus_tag="AC06IO_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIO9875003.1"
                     /translation="MSAKKNSIAQARLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFTPIVTKSDYESTCIDHMVANLFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQPEVM"
     gene            1673..3583
                     /locus_tag="AC06IO_001825"
     CDS             1673..3583
                     /locus_tag="AC06IO_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIO9875004.1"
                     /translation="MLLIEIILTIHCLLLLSSLFIGRAWLANKPSFKLKLLRFLLASC
                     IISPIIVHCVDSDQKLERKNYVSFDALQQYAKQPIFKTESSKFNHGSPSAFTSMNVNY
                     CQLLSMMFFLIVLFRSYHLLSGLSRLKLMLTEAIPYRTSGKLVIKVSHHCHVPFSILL
                     FNKAYIVLPVSIFSSSMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVVEAVSQCSLTSNREIACTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSTAGSLTSSRAKE
                     VDISHLDPRMQQIAEKQITTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSSETKNYDCHSPYYIAGKTFTNYNSNVESASLAEA
                     IAKSINVCLIRVSQEAGVSVIRKKLTEFGFDTSTWWQADRSDGLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSFDRGNSAIISETTSYSINHMLENAVTNGTGKLAVIPGVSVA
                     GKTGTVIENNDKYLALFAGYVPVDNPRYVVLVVIEDGYFSKNGKTLVSGGELAAPVFR
                     NVAMDALSSANR"
     gene            3633..4433
                     /gene="blaOXA"
                     /locus_tag="AC06IO_001826"
     CDS             3633..4433
                     /gene="blaOXA"
                     /locus_tag="AC06IO_001826"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HIO9875005.1"
                     /translation="MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDRGQNNGLTNAWLSSSLSISPAEQIQFL
                     QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHITDSKENATFASFRAKNDTLIQLFNLINELEK"
     gene            complement(4492..5367)
                     /locus_tag="AC06IO_001827"
     CDS             complement(4492..5367)
                     /locus_tag="AC06IO_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIO9875006.1"
                     /translation="MDKSPKLMHLLVYAVHLVLVSTCCFGEPNSPEKLLKITIANKTY
                     TLSELKKQFKTIRVIISHNPAYNFQKKTYDAFDINQILEQLMGADLQKNNADQVLLAQ
                     TLDKYVSQTPLLHFTTKGHAYLAYQETPGTISDNNITKDGRWSYINQHGKKENPGPFY
                     IVWDNTSTYPVSWPYQVISMQIVNKKELTFSHFLNPLNESASVQNGHHIFNNICSTCH
                     SLFYKGAQGRAPDLGMVTSYLTPSDISKLVKNGRGYMPPIGKNLSTEEINDLIKFLIW
                     VNKQASKLKCEINNQ"
     gene            5746..7206
                     /locus_tag="AC06IO_001828"
     CDS             5746..7206
                     /locus_tag="AC06IO_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIO9875007.1"
                     /translation="MNHKPIQFKDLSLIYPHKTCFQDFSCEIHFADRIALIGRNGSGK
                     STLLKMLCGLFLAPAGEIKIPHDVRFGHLPQVLEDCPDLSGGQRLNRVLSKILSDEPN
                     VLLLDEPTNHLDSRNRRSLMRMLEHYPGTLIIASHDTELINTVTDTLWHIDLGQVTVF
                     RGIYFDYQQILEDKKASIEQELSRIARQKKESHLALMKEQERNKRARVQGEKKIAQRK
                     WPTIRSHTKLGSAITTGNKRLSHINYKKQQLLEELSSLNFPEVIKPKFRLKGLGYHKS
                     LIRIQDASVAYEAVVILEDIHFHLSGCERVALHGDNASGKSTFVKAILGESQIKRTGE
                     WAVPDCNTIGYIDQHYQHLNFNETVLDLMRSNMPHASHTEIRAHLNDFLFRKNEEVEI
                     KVKNLSGGEKARLSMALIAANPPKLLILDEITNNVDLETRTHIIQVLREFQGAMLVIS
                     HDYDFLESIHIETRYQIHQGKIHHFNEKQLEVVHHD"
     gene            7199..8980
                     /locus_tag="AC06IO_001829"
     CDS             7199..8980
                     /locus_tag="AC06IO_001829"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9875008.1"
                     /translation="MTNYLPSHLGSFMWHFLKPYRGIVILFILFALLAGFWGPFNSLL
                     IKSFINTLTEKTSQDLSSLYWIAGLLVLNFIVFDNITWRALGYLNYKYQAIIKSQIIS
                     QTFEYVLGGSTQFFQDNLSGRIADQITTLADNLEIILHRVSVDFLRGASLLVVSFITA
                     YFVNVLFFYILFLWFVAFASFSIWMSARLVQLSDDHASSESQLSGQLVDSLANQSNIR
                     IFSRKIYEVERMNTFFRMVQRAFQRKELFIVLLCCAQGGMIAVMMGFASITLIHLYGK
                     GLVSIGDFALILGLSMELGHMMWYTMYQVDQFNQALGKAKQSLNALVIPHEIKDKSNA
                     SQLVVTQGQIEFSRVKFHYQGGYSLFQNKSVTIKAGQKVGLVGYSGSGKSTFVNLILR
                     LYEVVEGQILIDGQNLSEVTQESLRQAIAMIPQDPTLFHRSLIENIRYGRTDATDEEV
                     ILASRKAHAHEFISLLPEGYKTLVGERGVKLSGGQRQRIAIARAILKNAPILMLDEAT
                     SQLDSITESNIQESLWDLMQGKTTLVVAHRLSTLLHMDRILVFDKGHIIEDGTHTELL
                     NRGGLYKTLWNAQVGGFLPDQQNEKGE"
     gene            8984..9940
                     /locus_tag="AC06IO_001830"
     CDS             8984..9940
                     /locus_tag="AC06IO_001830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9875009.1"
                     /translation="MSVKQEQHLIKVVPYDSSWSMQFEQEAEQIKKALGNNCIDIHHI
                     GSTSVPGLAAKPVIDMIPVVLDISKVDGANAAMRALGYEAKGEYGIPFRRYFQKGDNR
                     RTHHAHIFELGNSEIDRHLKFRDWMRMHPKDREAYARLKQELARRYLDDITTYCLGKE
                     DFIATIDKKAGFNGLRMVKALTTREWDKVRHFRQFYFFDKAGLSDPYTWTFEHNAHVH
                     FVLFQGSEITGYAHLQLWSHNRAALRIIVIDELKRNHQYGTRFLALCEKWLKSQGYQS
                     LHVESSPDALKFYRNNGYIDMPFDDPDGYEGDPRDTAVGKIL"
     gene            9937..10464
                     /locus_tag="AC06IO_001831"
     CDS             9937..10464
                     /locus_tag="AC06IO_001831"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9875010.1"
                     /translation="MTQIEIVIKTQELSDIPVLVDAFQKANWQKPASLFEAYYQEQRK
                     SLRVVWVAYFREQIAGYVTLKWISQYQSFAHQKIPEIMDLNVLPSFRKQGVGTALLQA
                     AEEKAASESDVVGIGVGLYGGHDGGYGQAQRLYVNRGYIPNGLGVTYDYKPTVPGQVY
                     PLDDDFILWFTKKLR"
     gene            10530..11282
                     /locus_tag="AC06IO_001832"
     CDS             10530..11282
                     /locus_tag="AC06IO_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIO9875011.1"
                     /translation="MAYTVKQLAKISNVSVRTLHWYDEIGLLKPAYQGANGYRYYEEK
                     QCLKLQQILFFRELGFPLNDIQKLLMQNDFDHIKALRAHKQVLESDINRKNQLIVTID
                     KTIQHLKGKHKMSNEELYYGFDSLRQKQYEKELVKEGVVSQDWMDKYKKKINQWTQAD
                     KDRFIQEGKDINQALISAINNQLKPTSPEVQAIIHRHHAWVGWNPNKEGYIGLSQRYL
                     TPEFRKFYEQQHPDLLEFIIEGMLFYANKNLD"
     gene            11533..12480
                     /locus_tag="AC06IO_001833"
     CDS             11533..12480
                     /locus_tag="AC06IO_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIO9875012.1"
                     /translation="MTQWFVKDLSQLTGVSVQTLHHYDRIGLLKPSLRLANGYRVYSE
                     KDLLKLQQIIALKFFGFELSQIKTLLAEEGNALNHFNSQARVLEQKASTLLEGAKTLR
                     RIIDSVDKNQSIPWETIIQLIEVYKMTEHLEHGWVKEIFTPDELKQYAEFEKEMKANA
                     TPEQKAAMENNWSQLVDEVKNNLKQDPDSKIGIYLGKKIMDWVNGVYGKKYAHLRTKK
                     FEQGFGEGKGLDEVGLTPEIVSWMDKAMDAYWRDRIYGILDQVGKAPSASVLALWNEM
                     LIDMYGEETSRKNAIYEIALNDEEVSPEAKAWLRNVLNS"
     gene            12831..13811
                     /locus_tag="AC06IO_001834"
     CDS             12831..13811
                     /locus_tag="AC06IO_001834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875013.1"
                     /translation="MQLKSETKSTFHGKYSKSSPVVGARVKELELIYTHKEIFANYQK
                     VVYDQQKLNVIPLAFKEKADEISKEIYAAYIKKYSDHGLTIENSVDWNNVGFTRTYLS
                     DLENEWNTWFVDPLVATPNDKSHIEGQMYRPAYICALHELMHVEEMTPGISKGQYARF
                     ESVVEVLTVTKTIILMDEVYKKTFNLPMDIEVDYENSFKLFGKTIKQGEFANFYRTLE
                     AKYPSLAEALVSKQSLTFLNQTNVEPMNINMQVLGGFLAVVGYAAVALALLLNNASFG
                     TLGLVVTGLGVASIFGGVGLFANGIYKNERTTDNEILDASACLAGNTNSP"
     gene            14100..14525
                     /locus_tag="AC06IO_001835"
     CDS             14100..14525
                     /locus_tag="AC06IO_001835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9875014.1"
                     /translation="MNYQLELVVDTKLLEQQDKMIRDGIVNFNAPFTGSKPERYSIYV
                     KDNENNIVGGAIVYAHKSSIYVDALWVSEKYRGRGIGAELLRGVETEAIKRGIPESTL
                     DTFSFQAEGFYLKQGYKHLGIIKNYLEGHDRIYLRKKLK"
BASE COUNT         4683 a         2492 c         2970 g         4572 t
ORIGIN      
        1 gtttctgaac cctctggtta tctctttgaa aagagcttga ttttaggatt tctgcttagg
       61 gaatggttcc caactcaact tgttaataat tcagcagtta aagcggaaat gctggaaggt
      121 gagaaagggg tattcagtgc ttttaaaaat tataaagagt atcgctcatt tatgtcgaag
      181 gaagaattaa aatcaacgga atttggtgcg ttatatgaag cgaacgaggc ttttttagag
      241 tatttttata atcgctctga aagtacttta ataaataagg attcaccttt tgaaaaatat
      301 tttgttggct cgtcctcaga tgaagaggca attaaaaatt attgggacgc agaagggtat
      361 acattatatt gtcaacattt ggcgaaacct caggttaaat tatcttatat tgaaattatg
      421 accatgatga aggtcatcaa tcagccactt acaatatatg atcgttctac ttcctcagtt
      481 gtagccgagt atgtcaaccc aaaggtcaat ttacctgatt ttgaagtagc tattgatgct
      541 cttcaagggc attattttct tttaaaaact gagaatacgg aaaaagagtt agaagaatat
      601 gaaagaagtt atgctcaata taaaagagat agaagtgaaa tccttgcaca ctccgataaa
      661 cctgtttctt ccttattggt tcgagctact tgccccaaag gtcatttgga tgaagatcct
      721 tttattgcat taattgaaag cctttccgaa ataaacagcc ttagccaaat tgatactaat
      781 ttgaaaaacg agaatacaga tatctccaat tgcaacttcc ttttaaaggt aggtgcttct
      841 gtagttggta cctcttctgc actaataact ttaatgggaa tagccatatt atctggccaa
      901 tatgatcccg gaaacagtct ggccgaagag gcattagcta ctactgttgt atccagtatt
      961 gtgggtttag gttatgggct ttatagcttc tttagttcta gccccaaggt atcagagcgt
     1021 gattctacca atggattatc gcaaaaactt aaaatgaact aacctcctga attagcaaat
     1081 attgtctgta agctgatcgt gttgatatta gatatcaaca cgattgcacc tttgagcaaa
     1141 ctgttctcac ctgcatagaa gtatttttcc catgactatt ctaactctac tatttttata
     1201 gtagaatcct actataaaaa tagtagtagt gtatgtggtt attggagaga aacatgtcgg
     1261 caaaaaaaaa ttcgatagct caagctcgac ttcttacaga agtcgagctt gaattaatga
     1321 atattatctg gagccttgat aaggtaacta ttaaggaggt tgtttcacat ctgcctaagg
     1381 aaaggccttt ggcttataca accgtggcaa cagtgctaaa agtactggaa caaaagggat
     1441 ttcttgagtg tcaaaaaaat tcctatgctc atgtttttac acccatagtt acaaagtcag
     1501 attatgaaag tacttgtatt gatcatatgg ttgccaatct ttttgatggg gagccccgag
     1561 cacttgtcca aagacttttg ttagccagaa agttacagca tgatgacatt caagccattg
     1621 aagaagcact taaacaatta aacgctggtg aaaaacagcc agaggtgatg taatgttatt
     1681 aatagaaatt atattgacta ttcattgcct gcttcttctc agttcactat ttatagggcg
     1741 tgcctggcta gcaaataaac catcttttaa attgaaatta ctccgttttt tgttggccag
     1801 ttgcattatt tcaccaataa tagttcattg tgtagactct gatcaaaagc ttgaacgaaa
     1861 aaactatgta tcatttgatg ctttgcaaca gtatgcaaag caacccatat ttaagacaga
     1921 atcttcaaaa tttaatcatg gatcgccttc tgcctttaca tcaatgaatg tgaactactg
     1981 tcaactctta agcatgatgt tttttttaat cgtcttgttt cgtagttatc acttgttatc
     2041 aggtttaagt cgcttgaaat tgatgttgac tgaagcaatc ccatatcgaa cttccggaaa
     2101 attagttatt aaggtttctc accactgcca tgttcctttc tcaatattgc tatttaataa
     2161 agcctacatc gtattgccag tatccatatt ttcttcttcc atgaatgtga ggattgctat
     2221 tgctcatgaa gggcaacacc atcgtaatgg agattgttca tgggcctatt ttatcgaaat
     2281 catacgcatt atcttctttg gaaacccggg tgtaactcaa tggtttcgtg ttttagcaga
     2341 tttacaggaa ttgtcatgtg atgaagtatt ggttgggcat caaaaaattt cggcacatga
     2401 ctatggtcac tgtctattgc aagtggtaga ggcagtatcc cagtgctctc tgacatctaa
     2461 ccgggaaatt gcatgcacag tgggtatggc tttgaacaaa gaaaacgaag actgtaaatt
     2521 tataataagg agaatttcaa tgctaactgc ttattcaccc aatgcatcca gacctttgct
     2581 attagggata gtcttcgcag gattttctat cctgtctcca atctgcgttg cttattcaac
     2641 agcaggatct ttgacgagtt ccagggcaaa agaagttgat atttctcatt tagatcccag
     2701 aatgcagcag attgcagaga aacaaattac gacagccgtc aaacaatatc atgcaaaatc
     2761 tggagttatt gcaatagctg atccccaaac aggcaatatc attgcatttg ctgaatcaag
     2821 taaaaataag gggctagaga gctggaaatc acgtattttt tcacccggat ctactataaa
     2881 accttttatt gcagcagctg caatcaactc tgggaatagc tctgaaacaa aaaattatga
     2941 ttgtcattca ccttattaca tagcaggcaa aacatttaca aattataatt ccaatgttga
     3001 atcagcttcc ttggcagaag ccatcgcaaa atctatcaat gtatgcctta tcagagtttc
     3061 tcaagaggct ggggtgtctg taatccgtaa aaagctcact gaatttggtt ttgacacaag
     3121 cacttggtgg caagcagatc gaagtgatgg tttacaactg gcaatggctg cacttggaga
     3181 gaatatccct gtcacaatcg aatcgctaat aaaatcttat gccattcttg ctaacaaagg
     3241 acattcattt gacagaggaa atagtgccat tatttcagaa acaacctctt actcaataaa
     3301 tcatatgctt gaaaatgctg ttacaaatgg cactggaaaa cttgcagtta ttcctggtgt
     3361 ttctgtagca ggtaagacag gaactgtaat tgaaaataat gataaatatc ttgctttgtt
     3421 tgccgggtat gtgccagttg ataatccgcg ttatgttgta ttagtcgtga ttgaagatgg
     3481 ttatttcagt aaaaatggaa agacattagt ttcgggagga gaattggctg caccggtatt
     3541 ccgaaatgtc gcaatggatg ctctgagcag tgcaaatcga taactagctt atgggtttgc
     3601 atatccatat attcaaaaat gttgaggttg ttatgaagaa aatcattata tttttatgca
     3661 caggattatt attttgcgtt tctacttggg ctcaaggcac ctgtttttta gcaaaagaaa
     3721 atcaaactgt tttaaaacgt gagggcaatg attgcgatca acgctattcg ccagcatcaa
     3781 cttttaaaat tgcccttagc ctcatgggct ttgattctgg aatattaaag gatgagttgc
     3841 atcctgaatg gccatacaaa aaagaatatg aactttatct gaatgtatgg aagtaccctc
     3901 aaaacccgca tacctggatc agagactcct gtgtttggta ttcacaggca ttaactcggc
     3961 aattgggcat gaaacggttt aaagggtatg tcgacgcatt tcactacggt aatcaggatg
     4021 tttcgggaga cagaggtcaa aataacggct taaccaatgc ctggctttcc agctcacttt
     4081 ctatctcacc cgcagagcaa attcaatttc tacaaaaaat aatctataag aaactgccgg
     4141 tgagtcaaaa agcttatacc atgactaaaa atatcatgta catccaggag ttgcctggtg
     4201 ggtggaagct ttatggaaaa acaggaactg gtcgacagtt aacaaaagat aaaagtcaaa
     4261 aattgccttt gcaacatgga tggttcgtag gttggattga gaaggatgag cgtgtaatta
     4321 cctttgcaaa gcatattaca gacagtaaag agaatgccac ttttgccagt tttagagcga
     4381 aaaatgatac attgattcaa ttgtttaacc tgattaatga gctggagaaa taatccaaaa
     4441 gatggctggt tcagtgtttt ctgatccggc tatctaatct ctgaattata atcactggtt
     4501 gttaatttcg cattttagtt tggaagcttg tttattaacc cagattaaaa atttaatgag
     4561 atcattaatt tcttccgtag agaggttttt gcctatcggt ggcatgtatc ctcgtccatt
     4621 ctttacaagt ttactgatat ctgaaggcgt taaataactc gtcaccatac ctaagtctgg
     4681 tgcgcgtcct tgagccccct tgtaaaaaag agagtggcat gtggagcata tattattaaa
     4741 aatatgatga ccattttgca cagaagcaga ttcattcaat ggatttaaaa aatgagaaaa
     4801 agtcagctct ttcttattta ctatttgcat cgaaataacc tggtatggcc aactcacagg
     4861 gtaggtactt gtattatccc atacaatata gaaaggacca ggattttctt ttttaccgtg
     4921 ttgattaatg taactccaac gaccgtcttt ggtgatatta ttgtcagaga ttgttccggg
     4981 cgtctcttga taagcaagat aggcatgccc ctttgtcgta aaatgcaaca agggagtttg
     5041 agacacatat ttatctagtg tttgagctaa taatacctgg tcagcattat ttttttgtaa
     5101 atctgctccc atcaattgtt ctaaaatctg attgatgtca aacgcatcgt atgttttttt
     5161 ctgaaaatta tatgcaggat tatgtgagat aatcacacga atggttttaa attgtttttt
     5221 tagctccgac agggtatatg ttttattggc aatagtaatt ttaagtagtt tttctggtga
     5281 attgggttct ccaaaacaac atgttgagac taataccagg tgaaccgcat aaaccagaag
     5341 atgcattaat ttggggctct tgtccataag tccttcttgt ttgttgaaaa caaatcagat
     5401 ttttcccata gtcttactag ctattatggt ttataacagg agcgcttcca agtgatattt
     5461 cccagcgagc agaattattt aacttaaatc tgagacactt ataagacgcc aatggaacta
     5521 ttatgaaaag gaagagataa gccaggaaaa ataattttga atataatatt gacatcttca
     5581 actattttaa tttatatttc atctaatttt tttaccagtt aaaaggtagg tttaggccgt
     5641 catgaaaatt acatcttaag tacgcccaac tatagggcaa agtgaacaaa gtcctttgtg
     5701 acttttagtt tttactgtcc tggcagacat gcttaagagt aaatcatgaa ccataaaccg
     5761 attcaattta aagacctcag tcttatctat ccgcataaaa cctgttttca agactttagt
     5821 tgtgaaatac attttgctga tcgcattgca ctgattggtc gtaatggctc aggaaaatca
     5881 acgctactca agatgctttg tggtcttttt ttagctcctg cgggcgaaat taaaataccc
     5941 catgatgttc gctttggtca tttacctcaa gtgcttgagg attgtcctga tttaagtggc
     6001 ggtcagcgat taaaccgagt attaagcaaa atactgtcgg atgagcctaa tgttttgttg
     6061 ttagatgagc ccacaaatca tctggacagc cgtaatcgtc gttctttaat gcgcatgctt
     6121 gagcattatc ctgggacatt gatcatagcc tctcatgata cagaactcat caatacagtc
     6181 acagatactc tatggcatat tgatttaggt caagtgaccg tattcagagg aatttatttt
     6241 gattatcaac aaattcttga agacaagaaa gcatcgattg aacaggagtt atcaaggatt
     6301 gcacgacaga aaaaagaatc acatcttgct ttgatgaaag agcaggagcg taataaacgt
     6361 gcgcgtgtcc agggtgaaaa gaagatagcc caacgcaaat ggccaacgat tcgatctcat
     6421 acaaagctcg gttcagcaat tacaacaggc aataaacgat taagtcacat taactataaa
     6481 aaacaacaat tactcgagga gctttcctct ctaaactttc cggaggtaat taagcccaaa
     6541 tttaggttaa agggactcgg gtaccataaa tccttaatca ggattcaaga cgcttctgtt
     6601 gcctatgagg ctgttgtgat tcttgaagat attcattttc accttagcgg ttgtgaacga
     6661 gtggcattac atggggacaa tgcttcaggt aagtcgacct ttgttaaagc gatattgggg
     6721 gaaagtcaaa ttaaaagaac aggggaatgg gctgtgcctg attgcaatac aataggctat
     6781 atcgatcaac attatcaaca tttgaacttc aatgaaacag tcctggattt aatgcgctcc
     6841 aatatgcccc atgcatctca tacagaaatt cgtgctcatt taaatgattt tttatttcgc
     6901 aaaaatgaag aagtagaaat taaagtcaaa aatctttctg gtggtgaaaa ggcaaggtta
     6961 tcgatggcat tgattgctgc aaatccacca aaactactta ttttagatga gatcacgaac
     7021 aatgtagatt tggaaacacg cactcatatc attcaggtgc tgcgtgaatt ccaaggtgca
     7081 atgctggtta tttctcatga ctatgatttt ttagaatcca ttcacattga aaccaggtat
     7141 cagattcatc aagggaagat acatcatttc aatgagaaac aattagaggt agtgcatcat
     7201 gactaattat ttgcccagtc acttgggctc ttttatgtgg cattttttaa aaccatatcg
     7261 aggcatagtg atattattta tcttgtttgc tctattggcc gggttttggg gaccattcaa
     7321 tagcttattg attaagtctt tcattaacac tttgactgaa aaaacaagtc aggacctatc
     7381 ttctttgtat tggattgctg gattattggt attgaacttt attgtttttg acaatataac
     7441 ctggcgagca cttgggtact tgaactataa atatcaagcg atcattaaaa gccaaatcat
     7501 tagccaaaca tttgagtatg tgttaggagg aagcacacaa ttttttcagg ataatctatc
     7561 cgggcgaatt gccgatcaaa tcacgaccct tgctgataat cttgagatta ttttgcatcg
     7621 tgtgtctgta gattttcttc gtggcgcttc attattagtg gtttcattta ttacagccta
     7681 ttttgttaat gtcttgttct tttatatttt gtttctctgg ttcgtcgcgt ttgcctcatt
     7741 tagtatttgg atgtcagcgc gattagtcca gttgtctgat gatcacgcaa gttctgaatc
     7801 acagctttcc gggcagttgg tagatagcct ggcgaatcaa tccaacattc gtattttttc
     7861 aagaaaaatc tacgaagtgg aacgtatgaa cactttcttt cgtatggtac aacgggcttt
     7921 tcaaaggaaa gagctgttta ttgttttgtt atgttgcgca caaggtggaa tgattgcagt
     7981 gatgatgggt tttgcatcga ttactttaat ccatttatat ggcaagggtc ttgtaagcat
     8041 aggtgatttt gccttaattc ttggactttc catggagttg ggccatatga tgtggtacac
     8101 catgtaccaa gtggatcaat ttaaccaagc cttaggcaaa gcaaaacaaa gtttaaatgc
     8161 attggtgata ccccacgaga ttaaggataa aagcaatgca tctcaattag tcgttactca
     8221 aggacaaatt gaattttcca gggtgaaatt tcattaccaa ggcggttatt ctctgttcca
     8281 gaataaatct gtaacaatta aagcaggtca aaaagtgggt ttagtaggct attcaggcag
     8341 tggtaaatcg acttttgtga atctgatttt acgactttat gaggttgtgg aggggcagat
     8401 tcttattgat ggtcaaaacc tatctgaagt cacgcaagaa agtttaaggc aagctattgc
     8461 tatgattcct caagacccaa ctctttttca tcgaagctta atagagaaca tacgctatgg
     8521 cagaacggat gcaacggatg aggaagtgat attagcctcc aggaaggctc atgcacatga
     8581 attcataagt cttttaccag aaggctataa aaccctggta ggggagcgtg gggttaaact
     8641 atcaggtggt cagcgccagc gcattgcgat tgcccgagcc atcttaaaaa atgctccgat
     8701 tttaatgctg gatgaagcca cgtctcagct tgattccatt actgaatcca atattcaaga
     8761 gagtctctgg gatttaatgc agggaaaaac aaccctggta gttgctcatc gcttgtctac
     8821 cctcctgcat atggaccgaa ttttagtatt cgataaaggc catattatcg aagacggtac
     8881 gcacaccgaa ctattgaatc gtggcggttt gtacaaaacc ttgtggaatg cgcaggttgg
     8941 tggttttttg ccagatcaac agaatgagaa aggagagtaa gttatgtcag tcaagcaaga
     9001 gcagcatctc atcaaagtag taccttatga ttcgagttgg tctatgcaat ttgaacagga
     9061 agcagaacag ataaaaaaag cgttaggtaa taattgcatt gatattcatc atattggttc
     9121 cacctcagta cctggtttgg ctgcaaaacc agtcatcgat atgattccag ttgttttaga
     9181 tattagcaaa gtagatggag ctaatgctgc catgcgagca cttggctatg aagcaaaagg
     9241 agaatacggt attccctttc gccgttattt ccaaaaggga gataatcgac gaactcatca
     9301 tgctcatatt tttgaacttg gaaactctga aatagatcgg catttaaagt ttagggattg
     9361 gatgagaatg cacccgaaag atagagaagc ttatgcacgt ttaaaacaag agttggcgcg
     9421 ccgatatttg gatgacatta ccacctattg tctgggtaaa gaagatttta ttgcaacaat
     9481 tgataaaaaa gccgggttta atggattaag aatggttaag gcactaacaa cacgtgaatg
     9541 ggataaggtg cgtcatttca gacaatttta tttttttgac aaagcagggc tttctgatcc
     9601 ttatacctgg acttttgagc ataatgcgca tgttcatttt gttttatttc aaggaagtga
     9661 aatcacaggt tatgctcatc tgcaattatg gtctcataac agagcagcat tacggattat
     9721 cgttatcgat gagcttaaaa gaaaccatca atatggtacc cgttttttgg cattatgtga
     9781 aaaatggctc aaaagtcagg gctatcaaag tttgcatgtc gagtcgtcac cagatgctct
     9841 gaaattttat cgaaataatg gttatatcga tatgcctttt gatgatcccg atggctatga
     9901 aggtgatcca cgggatactg cagtaggaaa aatattatga ctcaaataga aattgtcatt
     9961 aaaacacagg agttatccga catccctgtt ctggttgatg catttcagaa agcaaattgg
    10021 caaaaacctg cttcattgtt tgaagcatat taccaagaac agcgaaagtc tcttcgggtg
    10081 gtatgggtgg catattttcg agaacaaatt gctggctatg ttactctaaa atggatatca
    10141 caataccagt cgttcgctca tcaaaaaatt ccggaaatta tggacttaaa tgtgctgccc
    10201 tcttttcgta aacagggagt gggaactgct ctactgcagg ctgctgaaga aaaagcggca
    10261 agtgaaagtg atgtcgtagg tattggtgtt ggtttatatg gcggccatga tggaggatat
    10321 ggacaagcac aaaggcttta tgtcaatcgg ggttatatcc ctaatggttt aggtgtcact
    10381 tatgattata agccaactgt tccggggcag gtctatcctt tagatgatga ttttatttta
    10441 tggtttacaa aaaaattaag atgaaatgct tgacccttac gttacgtgag cacttatctt
    10501 taaatggaat catttgagag gacagtttaa tggcttatac ggtaaaacaa ttagcaaaaa
    10561 tatcgaatgt aagtgtgcgc actttacatt ggtatgatga aataggctta ttaaaaccag
    10621 cctatcaagg tgctaatggc tatcgttatt atgaagaaaa gcagtgctta aaattacagc
    10681 aaattttatt cttcagagag ctgggattcc ccttgaatga tattcaaaaa ctgcttatgc
    10741 aaaatgattt tgaccacatc aaagcattga gagcgcataa acaggttttg gaaagcgata
    10801 tcaatcgaaa aaatcaattg attgtaacga ttgataaaac cattcaacat ttaaaaggta
    10861 agcataagat gtctaatgaa gaattatatt atggctttga ttcactgcgg caaaaacaat
    10921 acgaaaagga attagttaaa gaaggcgtgg ttagccagga ttggatggat aagtacaaga
    10981 aaaaaataaa tcaatggact caagctgata aagaccgatt tattcaggaa ggcaaagata
    11041 tcaatcaagc attgatttcg gctattaata atcaactcaa gcctacttct ccggaggtac
    11101 aagccattat ccataggcac catgcttggg taggttggaa cccaaacaag gaagggtata
    11161 ttgggttaag ccaaagatat ttgactcctg agtttagaaa gttttatgag cagcagcatc
    11221 ctgatctgct cgaattcatt attgaaggga tgctgtttta tgcaaacaag aaccttgatt
    11281 aagttgagag aagggctgtg tcttatcagc cctatatata ttggattgag aacattgtgc
    11341 gtattagcag aaataatact gatttatgca tgccagctta tcatgttgtt ccaaaccagg
    11401 agcttttgtg agttttttgc ttaataattg atctcttggt aggttagaaa aataaagatt
    11461 caagaaattt taaaaactgc ttgaccctat tgttgctata ggcattacct ttgtcttgaa
    11521 gggggaaaca caatgacaca atggttcgta aaagatttaa gccaattaac tggtgtatca
    11581 gtgcaaacgt tacatcacta tgaccgtata ggattgctaa agccttcatt acgacttgcc
    11641 aatgggtacc gtgtttactc tgagaaggat ttattgaaat tacaacagat tattgctttg
    11701 aagtttttcg gttttgagtt gtcacaaatc aaaaccttac tcgctgaaga aggtaatgcg
    11761 ctcaatcatt ttaacagcca agctcgggtg ttagaacaaa aagcatccac tttacttgag
    11821 ggagctaaaa ctttgagacg catcattgac tctgttgaca aaaatcaatc tatcccatgg
    11881 gaaaccatta ttcaacttat agaggtatat aaaatgacag aacatcttga gcatggctgg
    11941 gtaaaggaaa tattcacgcc ggatgaatta aaacagtacg ctgagtttga aaaagaaatg
    12001 aaagccaatg ctacgcctga gcaaaaagcg gctatggaaa acaattggtc tcagctagtt
    12061 gatgaggtaa aaaataatct gaagcaagat ccagattcaa aaataggaat ttatttagga
    12121 aaaaagatta tggattgggt taatggggtc tacggtaaaa aatatgccca tttaaggacc
    12181 aaaaaatttg aacagggctt tggggaaggg aaaggtcttg atgaggttgg attaacacca
    12241 gaaatcgtat catggatgga taaagccatg gatgcttact ggagagaccg aatctatggg
    12301 attcttgatc aagtaggaaa agctccttca gcaagtgtat tagctctttg gaatgaaatg
    12361 ctgattgata tgtatggtga ggaaacgtca cgtaaaaatg ccatatatga aatcgcctta
    12421 aatgacgagg aggttagccc tgaggcaaaa gcttggctta gaaatgtttt aaattcctaa
    12481 aaacatcaac aacgctgttg gatcggtatt gctttaccga tccaaaaaat cactaattat
    12541 ttatggtttt gcaaaattat ttatcatttt aagcagactg accatcccca atgttttaaa
    12601 aatgaatttt tatcctcttc aaactgcatt gccattgtat ttttccctaa tagcatctgt
    12661 tcaagagaat aacgacaatc ttcaaaaaat cgatgtaatc gatagaattc acagagacct
    12721 ttattgattg tgaatttagg ggcctttgca caggacaagc tgccttaata aaaccttaat
    12781 attcaaaatt tataattgcg taatctattg ttttatgtaa tgcacgtaga atgcaattaa
    12841 agtcagaaac aaagagtact tttcatggaa aatactctaa atcgtcaccc gtagtaggtg
    12901 ctagagtaaa agaacttgag ctgatttata cccataagga aatatttgct aattaccaaa
    12961 aagttgtata cgaccagcag aaattaaacg ttatacctct tgcgtttaaa gaaaaagcgg
    13021 atgaaatttc aaaagaaata tatgcagcat atattaaaaa gtattctgat catgggttaa
    13081 ccatcgaaaa ttcagtagat tggaataatg ttggctttac aagaacttat ctatctgact
    13141 tggagaacga atggaataca tggttcgtag atcctcttgt agcaacacca aatgacaaat
    13201 ctcatataga aggacaaatg tatcggccgg catatatctg tgctttacac gagttaatgc
    13261 atgtagaaga aatgaccccg ggaatttcaa aaggtcaata tgctcgattt gaatcagtag
    13321 ttgaggttct aaccgtaaca aaaactataa ttttaatgga tgaagtctac aagaaaacct
    13381 ttaatttacc catggatatt gaagttgatt atgaaaattc atttaaatta tttggaaaaa
    13441 caattaaaca gggagagttt gcaaattttt atcgtacctt ggaagctaaa tatccctctt
    13501 tagcagaagc gttagtatca aagcagtctt taactttttt aaatcaaact aatgttgagc
    13561 caatgaatat taacatgcag gtattaggtg gttttttggc agtagttggt tacgctgcgg
    13621 tagcccttgc gcttcttcta aataatgcat cttttggtac tttaggacta gttgtaacag
    13681 gtttgggagt agcttctatt tttggaggcg ttgggttatt tgctaatggt atatacaaaa
    13741 atgagcgaac aacagacaat gaaattttag acgcttcagc gtgtcttgca gggaatacta
    13801 attcaccata aaaatcgtct ttgtttctta accctctgca tggttacctt ttctaaaatt
    13861 taggtggatc tatagcttca ggcatcccca atgaagtcat tttattatga ggtttctgca
    13921 aatttgcaga aacctcacgg attactctac tgcatcagga atagctatct cacattttca
    13981 gactatataa agcatctctc ctttgagcat aattgaataa atatttttat tgtttttgac
    14041 acaataacat gaattttcaa atttttattt actgttaata tcttaaggaa taattataaa
    14101 tgaattatca attagagctt gttgtagata caaagctttt agaacaacaa gataaaatga
    14161 ttcgtgatgg aatagtgaat tttaatgctc catttacagg atccaagcca gagcgttatt
    14221 caatctatgt gaaagataac gagaataata ttgttggtgg ggcaattgtg tatgctcata
    14281 aaagttcaat ctacgttgat gccctctggg ttagcgaaaa atatcgtggg cggggtatcg
    14341 gtgctgaact tttacgaggt gttgaaaccg aggcaattaa aagaggaatt cctgagtcaa
    14401 cattagatac tttttctttc caggctgaag gcttttattt aaaacaaggc tataagcatt
    14461 tgggaattat taaaaactat ttagaagggc acgatcgcat ttatttacgc aaaaaattga
    14521 agtaattgta ctgcttggtt gtagtttaat ctggcgcagt catgtttgaa ggtgagtcaa
    14581 aatggtaaat atccatagag atggtttttg atcagaataa gtattatgaa aactttgtga
    14641 tgacttgaaa gacaaatttc aagcatactg atattcttga tattaagaaa aatttgttta
    14701 tcaatatgaa atgaaaa
//
LOCUS       DBNCWK010000069        14491 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.069, whole genome shotgun sequence.
ACCESSION   DBNCWK010000069 DBNCWK010000000
VERSION     DBNCWK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.069"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            97..1377
                     /locus_tag="AC06IO_001836"
     CDS             97..1377
                     /locus_tag="AC06IO_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIO9875015.1"
                     /translation="MCAAHHQKNKYIFSTPLIYAAMIGLGIISGLSDLPFLKETGLLI
                     SDLFIKLFKCISLPIISLSIIVTLANYQTDSLMKSVWQRTIKYTFATTLVAALISCFL
                     YILIHPSTVQVNLDAANPKSTSSLGYLGYLSNMIPTNLLSPFLEQQVMGVLLLSIVIG
                     FAVRQIPETEPRETIIRFFRGAHGMFLVMTKWIIKLIPLGLFGFITSTVIQLRSGMDI
                     KGIGQYLMIIVLANLIQGLVILPLWLKSNGIKPFSSMRAMLPALSVAFFSKSSVGTLP
                     VTMNTVEANLKVNPQVSRFVLPLCTSINMNGCAAFIFATVIYLMQSHGIQVSYATMGI
                     WVLVSTIAAIGNAGVPMGCFFLSVSLLSSMNVPITLMGVILPFYGLIDMLETALNVWS
                     DACVTKVVNDKAEGGDGVVGIRPKERSLLNAELG"
     gene            complement(1700..2833)
                     /gene="dapE"
                     /locus_tag="AC06IO_001837"
     CDS             complement(1700..2833)
                     /gene="dapE"
                     /locus_tag="AC06IO_001837"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIO9875016.1"
                     /translation="MTDITQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNN
                     GPVSNFFACYGKIGPLLVFAGHTDVVPVGEVSKWDTDPFSLEEKNGMLYGRGVADMKG
                     SLACMLHMARRFIKTYPSFPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCI
                     VGEPSSSLKTGDVIKIGRRGSLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSM
                     QWDNGNAYFPPTSMQITYIHCGGHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAF
                     THHKLNPTIEWRFNGEPFLTNKGILLESCKQTVLEHIGTLPELSTSGGTSDGRFIAPY
                     GVEVIELGLVNATIHQVNECTSLQDLNTLETMYFSICEKLFID"
     gene            complement(2826..3656)
                     /gene="dapD"
                     /locus_tag="AC06IO_001838"
     CDS             complement(2826..3656)
                     /gene="dapD"
                     /locus_tag="AC06IO_001838"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIO9875017.1"
                     /translation="MNSLQALIEQAFENRQNLSLNTVSSDLINAINEVLSGLDNGQFR
                     VAEKINGEWTVHQWLKKAVLLSFKLFPNQIIDAGFCKFYDKIPLKYTDSSNEQFQQSG
                     VRVVPHAMVRRGAYIAKNTVLMPSYVNIGAYIDEGVMVDTWATVGSCAQIGKNVHISG
                     GAGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEKNSVISMGVFLGQSTKIYNRI
                     TGEVSYGRIPAGSVVVAGNLPSHDGSHSLYCAVIVKQVDEKTRAKVSINDLLRANQDD
                     "
     gene            complement(3660..4553)
                     /locus_tag="AC06IO_001839"
     CDS             complement(3660..4553)
                     /locus_tag="AC06IO_001839"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIO9875018.1"
                     /translation="MQKNKQNGGQLHAIAAIFALIVSTTLCFIPILFVGLLKLFPNKN
                     WQSLCTRCVDRIATFWCGINNFYVAKAQKIHWEISGLNNLTPKDWYLVVANHQSWLDI
                     VILQRLFNRKIPVLKFFIKDQLKWIPLLGFSWWAMGCPFMKRYSKEYLAKNPHKQGKD
                     IISTRKAIETFKRTPASIMNFVEGTRFTKVKKEQQQSPYNYLLKPKAGGISFIINSMG
                     QQIKSLIDVTIVYAEKNHSLWDFLCKRVKKVKIIIRQLPIPAQFTSANLINDFQAQNA
                     FKEWLNEQWAIKDNLIASLQE"
     gene            complement(4620..5771)
                     /locus_tag="AC06IO_001840"
     CDS             complement(4620..5771)
                     /locus_tag="AC06IO_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIO9875019.1"
                     /translation="MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLG
                     YEYSRTQNPTRKAYEDCIASLESGQKGFAFASGMAAINTVIDLLDSGDHVLAMDDLYG
                     GTFRLFDKVKTRTSNLSFSFIDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKI
                     AAIAKKHNLITVADNTFATPWIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNP
                     MLSDKIAFLQNSCGAVAGPFDSFLVLRSLKTLSLRMQRHCENANHLANWLNGHPKIEK
                     VIYPGLKSHPQYSLAKEQMNDFGGMISLVLKGGLEDAKRFLARCELFTLAESLGGVES
                     LIEHPAIMTHASIPVEQRKALGIEDGFIRLSVGIEHIDDLRADLEHALG"
     gene            5920..8235
                     /locus_tag="AC06IO_001841"
     CDS             5920..8235
                     /locus_tag="AC06IO_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIO9875020.1"
                     /translation="MRRQEINYEKVAKAAEKIKKRGIEPSVNEIRDELGLVGNHPQLS
                     ILLEEWYHNQPEFKRKSHTPLTENINLNTDEIREKNVELEKSISLLRATLESTADGIM
                     MVNGHGAVVDWNQKFVEMWRIPSYMMESGKESISFEYILEQLIDPQSLIADVQYLYQN
                     LEWQGELPELHFKDGRIYERFTQPQRVGSQIVGRVYSFRDVTQKRMALDELRIRERAI
                     EASTHGVVIIDVTKNENKVIYVNRAFERITGYGEQHALGKGLLTLLGSNLEEVNHKRI
                     ELAIRESKEETIEMESIKRNGEFYWCEISVAPVKDSFGYVKHYICILNDVTQRRDMED
                     QLLQQATYDSLTNLPNRVLLMDRVEQAILQARKNKAILAFLFLDLDRFKLTNDTLGHS
                     MGDKLLQAIANRLLIVTEDFDTVARLGGDEFVILLTDIDNMLEAETIAQNILKIIEKP
                     IQIDQHSLKITGSLGISFYPRDGDDYESLMKSADLSMYHAKDTGRNNYRVYEPEMNRR
                     VINHMQLDNALRDALKNDELFLVYQPLIDLKQSRVVGFEALMRWHSKILGLVSPADFI
                     PMAEENGMILEMGEWAMKQACMQVKEWHKAGFKNLSIAVNLSGRQFRQKNLPEVVSRV
                     LKSSGLQSRFLELELTESLLIEDIDHVVDTMYALKDMGTKLVIDDFGTGYSSLSYLKQ
                     FPVDKLKIDRSFITEMVSNQNDAAIAKAIINLGHSLNLQVLAEGVENEFQRDFITSHG
                     CDYAQGYFFKAPDTPENILEFLKSLSESKIK"
     gene            8537..9886
                     /locus_tag="AC06IO_001842"
     CDS             8537..9886
                     /locus_tag="AC06IO_001842"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9875021.1"
                     /translation="MKHITIIGAGLAGTLCGLYLARRGYEVELFESRPDIRNSPTDYG
                     RSINLALSCRGITALKAMNLLSEVNKIMVPMRARAIHEANGEVHYQPFGRHIDEYINA
                     ISRSDLNALLLNKAELCPNIKLHFNMKLHSLDIHNKKIKFENKNGDFVEVSYHRLIGA
                     DGAPSHVRDMLKNEGIVSASRDFLSHGYKELSISKKHTKGMAREHLHLWPRDSFMLLG
                     NPNPDDSITGSLFLANEGKDSFAELNNEEKLHLFFKTQFPDAYAAMPNLVQEFFGNPT
                     GHLSTIQCSPWYYKDECLLIGDAAHGIIPFFGQGMNSAFEDCRILDELLDEYQDDWSR
                     VNPVFYEHRKVNTDAIAKMSMDNYHEIHSDIRNPKFILQKQIERELMLRYPEHYVSMH
                     VLVMFTNTPYAKAMAIGELQSGLLEQICFPITDIKELNWQEVEKLLSVYDKKLAKII"
     gene            10103..10855
                     /locus_tag="AC06IO_001843"
     CDS             10103..10855
                     /locus_tag="AC06IO_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875022.1"
                     /translation="MKTVQAYIDSKQHEFMNHPFFNILEELNSIEEISYFVPELTFWA
                     MTFQDILRINEERVTDPYLKKVARHHRLEDAGHEKWFLSDKKYMGEICDNKACDRDDV
                     AWLYSKDSQLVRDAAYSIMSEIYKIEDENLNIVLLLTLESSGHVFFEKVVKQVKKTGE
                     DKNLKYFSSSHLEVELAHALFEEEMERKVYSRRLPISTRREALRMVDRCYDAFSKMFD
                     GLILACNKRLELAKERNQHVAKSVELRENQAV"
     gene            10812..12182
                     /locus_tag="AC06IO_001844"
     CDS             10812..12182
                     /locus_tag="AC06IO_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIO9875023.1"
                     /translation="MSQNQWSLEKIKQYKEDTGQALLSDEQSLVLFGQDFGKLIQSQP
                     VAVSAPQSIESLQSLILFANQYHLPVTIRGNGLSQGGQSLPVPGGLTISMQSFNKPLD
                     LSEDLIWVEANTSWKNLLEKSLLNNKAPYVLPYNCNLSVGGVLSAGGIGASSFKYGVI
                     NAYVSALEVVDGLGRKQIVEKNSPLFQACLSGQGRFGVITKACIQLRSVQPRVKTFFL
                     VYADQNQWFEDIYKIQGKVDYMEIFCSPSIQGARLKEDKRIPMAYWLYGLHLSVEYDR
                     HAENILGQLKPWNVLNIQEESILSYFLRHNSRFDMMKLTGQWDLLHPWYECFVPTSFL
                     TGVLSQLLEELPLHYASLVHVVPIAKQKAGFMMLPDCDSICEFMILNPGVPYSLEESC
                     LKAIENLDKHLLQNNGKRYLSGFLGTELPDNYWLKHFGEKYDSWVGLKKQYDPAGIFS
                     SMLHHI"
     gene            12532..13041
                     /gene="lspC"
                     /locus_tag="AC06IO_001845"
     CDS             12532..13041
                     /gene="lspC"
                     /locus_tag="AC06IO_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretory system protein LspC"
                     /protein_id="HIO9875024.1"
                     /translation="MKFDIQFLFSANYAKWIIISLISLFGILIIFEWISLFYPLTYVR
                     AIPVSNTKSIAGMREENLNYIFNTSLFGVYVPADLNEDNVKQSMLNVTLVGILFADKI
                     EESQVIIRSASGEEKTYNVGDKIPGGATIKRIMPGGVLVERDGTLESLSLPKNDLTFE
                     PVAKPLKEE"
     gene            13047..13424
                     /locus_tag="AC06IO_001846"
     CDS             13047..13424
                     /locus_tag="AC06IO_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIO9875025.1"
                     /translation="MQRIFKLFILIIFIPMMAACIHSTLNLREGIQCFKAQDYRKAFI
                     RLKPEAEKGNPDAQYAVGYMYYYGKGVVEDRKKARFWITCAARAGQPDAIVALGILDR
                     QARRDRELFQDNNLQHFPVDNGL"
     gene            complement(13503..14462)
                     /locus_tag="AC06IO_001847"
     CDS             complement(13503..14462)
                     /locus_tag="AC06IO_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIO9875026.1"
                     /translation="MNPILNLIISFIALLWAANHLVAGASGLAIRFQLSPLMIGLTIV
                     ALGTSAPELFISIISSLKDKNDLAIGNAIGSNIANIGLILGIIILIKPTSFHFNKLKK
                     VYPILIIVMLFAYSLILDGYLGTIDGCLFLIGCIAVLCYFIYLASHSPKRDLSVNEFR
                     PAILSYRSTPSNLLSIAIGLLILPICSKYLVYNASEIAKWAGISEFTIGLTIIAIGSS
                     LPELTTSLVAAIKGEDSIAIGTIIGSNIYNLLLILALPGLLNPTKISSVVLWRDMPVM
                     LSITLLLLFLNYYYQKKTSSWPGGILLLVYFCYMASLIINAHS"
BASE COUNT         4539 a         2433 c         2926 g         4593 t
ORIGIN      
        1 aattcctcac tatttcactc catatatcaa gacatctgtt gttgctgcta atcctacttt
       61 gcttaaataa ttaattaatg ttttaaggat tccgttatgt gtgcagcaca ccaccaaaag
      121 aataaatata tttttagtac cccactaatt tatgcagcga tgattggact tgggataata
      181 agtggattat ccgatttgcc ttttttaaag gaaacagggc ttttaatttc tgatttgttt
      241 attaaattgt ttaagtgcat tagtttacca attatttcct tgtcgattat tgtaactctg
      301 gcaaactatc aaacggatag tttgatgaaa agtgtatggc aaagaacaat taaatatact
      361 tttgctacaa ctttagttgc agcattaatc agctgttttt tatatatttt gattcatcca
      421 agcactgttc aagtcaattt ggatgcggcg aacccaaaat ctaccagcag tttaggatat
      481 cttggctatt tgagtaacat gataccgact aatttattat ctccattctt ggagcaacag
      541 gttatggggg tattgctgct tagcattgtt attggttttg ctgttcgtca aataccggag
      601 acagagccac gggaaactat tattcgattt ttccgcggtg ctcatgggat gtttttagtg
      661 atgaccaaat ggataattaa attaatacct ttggggttat tcgggttcat tacttctact
      721 gtaattcagt tgcgatcagg tatggatatt aaagggatag gccaatactt gatgattatt
      781 gttttagcta atttgataca aggattagtc attctgcctt tatggctgaa atcaaatggt
      841 attaaaccat tctcttcgat gcgggcaatg ttacctgcgt tatctgttgc ttttttttcc
      901 aagtcatctg tcggtacttt gcctgttaca atgaatactg tagaggctaa tttaaaggtt
      961 aaccctcaag tcagtcgttt tgttttacct ttatgcacaa gcattaatat gaatggatgt
     1021 gctgcattta tttttgccac agtcatttat cttatgcaaa gtcatggcat ccaggtttct
     1081 tatgctacta tggggatatg ggttttagta tcaacaattg cggcaatagg gaatgccggc
     1141 gttcctatgg gctgcttttt cttaagcgtt agtttgctct ccagtatgaa tgtgcctatc
     1201 acgcttatgg gagttattct tcctttctat ggtttgattg atatgttgga aactgcctta
     1261 aacgtatggt ctgatgcttg tgttaccaag gttgttaatg ataaagcaga aggtggtgat
     1321 ggagtggtgg gcattagacc aaaagagcgt tcattattaa atgccgagtt aggttaaagc
     1381 cagcaaatct tcaagcgcaa atgagtgtag cttacatata agtctccaga aagttttttt
     1441 gagacttata tctatgaaat cagtttgtaa tttataagag atcagctcac tacctggaac
     1501 ggccgtattt ggttcatcgc gaacgaaaat aatccttcaa aaatgctcac agaacaatct
     1561 atactgtgct ttttcaagga ttatttttat ccaaactaaa tgcaaatctg accgccagag
     1621 gatatttaaa gatgaggtta ctaacctatt actgtaattt ggactgttgg tgcgaaccct
     1681 cattacagaa ttggtttgat taatcaatga ataatttctc acagattgaa aaatacatgg
     1741 tttcaagtgt attcaaatcc tgtagagaag tgcattcatt aacctgatga atggttgcat
     1801 ttaccagtcc cagctctatg acctcaaccc cataaggtgc tataaaacgc ccatctgaag
     1861 ttccaccact tgttgaaagt tcggggagag tcccaatatg ttcaagcact gtttgtttgc
     1921 agctttctag caatatgcct ttattcgtta agaatggttc gccattgaat cgccattcta
     1981 tagtggggtt aagtttatgg tgtgtaaagg cattaattac tctcgttttt aatgactcgt
     2041 ctgtttgctc tgtcgaatat ctaaagttca aatgcagatt taattcgcct gggataatat
     2101 tacccgcatg ccctccacaa tgaatataag ttatttgcat ggaggtagga ggaaaataag
     2161 cattgccatt atcccattgc attgaagtga gttcggctaa tactgggcta attctatgaa
     2221 ttggattgtc agccaaatgc ggataggcaa catgaccttg ctttccactc agatgtattt
     2281 ttgcacttaa tgatccacgt cttccaattt tgatgacatc accagttttc aagctgcttg
     2341 aaggttcacc tactatgcaa taatcaataa caatcccttg ctgctccagt ttttgcatca
     2401 cataaggggt gcccaaatta aattcatcgc cttcttcgcc gctggtgatc agaaatccta
     2461 gtctgcctgg aaaggaagga taagttttaa taaatcttct ggccatatgc aacatacagg
     2521 ccaggctgcc tttcatatca gcaaccccac gaccatagag catgccattt ttttcttcga
     2581 gagagaaagg gtctgtatcc cattttgata cttcaccaac aggaactaca tcggtatggc
     2641 ctgcgaacac caataaaggc ccaattttgc catagcaggc aaaaaaattg gatacgggcc
     2701 cattattcag ttgttgacaa gtaaatccca attgctccag aaattgaatc atgtattttt
     2761 ggcagccagc atcttctggt gtaatagaag gaaagccaat gaggtcagtc agaatttgcg
     2821 taatatcagt catcttgatt ggctcttagt aaatcattaa tgcttacctt cgcgcgagtt
     2881 ttttcatcca cttgtttaac aatgacagca caatataaac tgtggcttcc atcgtgactt
     2941 ggcaaattac ctgcaacaac tactgagcca gccggaattc tgccgtagct tacttcgcca
     3001 gtgattcgat tgtagatttt agtgctttgg cctaaaaata ctcccattga tatgacggag
     3061 tttttttcta cgataacccc ttccactatt tcagagcgag caccaataaa gcaattgtct
     3121 tcaatgatgg tcgggttcgc ttgtaatggt tctaaaacac ctccaatacc cgcgccgcca
     3181 gatatatgta cattttttcc tatttgggcg caagaaccaa cagttgccca ggtatcaacc
     3241 attactccct catcaatata agcacctata ttgacataag aaggcattaa aacagtattt
     3301 ttggcaatat atgctccacg tcgcaccatg gcatgaggaa caactcttac ccctgattgt
     3361 tgaaattgct cattactaga atccgtatat tttagcggta ttttatcgta aaacttgcaa
     3421 aaacccgcgt ctatgatttg atttgggaaa agtttaaagg ataagagaac tgccttttta
     3481 agccattgat gaactgtcca ttcaccattt attttttcag cgactctgaa ttggccatta
     3541 tcaagaccac tgagaacttc attaatggca ttgatcaaat ctgaagaaac tgtgttgagc
     3601 gataaatttt gacggttttc aaaggcttgt tcaattagag cttgaagtga gttcatgttt
     3661 tattcctgta aagaggctat taaattatct tttattgccc attgttcatt taaccattct
     3721 ttgaatgcat tttgtgcctg aaaatcgttg ataagatttg ccgatgtaaa ttgtgcaggg
     3781 atagggagtt gacgaataat gattttaact ttcttgactc gtttacaaag aaaatcccag
     3841 agagaatggt ttttttctgc ataaacgata gtgacgtcaa tcaggctttt tatctgttgt
     3901 cccatcgagt taataataaa actaataccg cctgctttgg gtttcaataa ataattataa
     3961 ggggattgtt gctgttcttt ttttacttta gtaaaacggg ttccttctac aaaattcata
     4021 atagaagccg gggttctttt aaaagtttct atggctttgc gagttgaaat tatatctttt
     4081 ccttgtttat gagggttttt agccaaatac tccttactat accgcttcat aaaggggcaa
     4141 cccatagccc accaggaaaa gccaagtaga ggaatccact ttaactgatc tttgataaaa
     4201 aattttaaaa cgggtatttt tctgttaaat aaacgttgaa gaatgacaat atccagccaa
     4261 ctttgatgat tggcaacgac taaataccaa tctttaggag ttaaattatt taacccggag
     4321 atttcccaat gtattttttg tgctttagcc acataaaagt tatttattcc acaccaaaaa
     4381 gtagcaatcc tatcaacaca acgagtgcaa agggattgcc agtttttatt aggaaataat
     4441 tttaataatc caacaaataa tatggggata aaacaaagag tagtggacac tatcaatgcg
     4501 aaaatagccg caatcgcatg gagctgcccg ccattttgtt tatttttttg catgaaatac
     4561 ttctcctata cacatctgtc ctggttcttt ttaacagtca gaaccaggat ttacatcttt
     4621 taacctaaag catgctccag atcggcacgc agatcatcaa tgtgttcaat accaactgat
     4681 aatcttatga aaccatcttc tatacccagg gcttttcgtt gttcgactgg aatagaggca
     4741 tgggtcataa ttgcagggtg ttcaattaaa ctttctacac cccccaggct ttcagccaaa
     4801 gtaaataatt cgcagcgagc taaaaatcgt ttggcatctt caagaccacc tttaagtacc
     4861 aaagagatca taccaccgaa atcgttcatt tgttccttgg caagagaata ttgcggatga
     4921 ctttttaatc cgggataaat gactttttca atcttggggt ggccgttaag ccagttagcc
     4981 aggtggttag cattttcaca gtgcctttgc atacgaagag ataatgtttt taagcttctc
     5041 agaaccagga aactgtcgaa agggccagct acagcgccgc atgaattttg caaaaaagca
     5101 attttatcag acaacatggg attgtcaccg accacaacga caccactgat tacatcagaa
     5161 tgtccgttca gatatttggt tgctgaatga agtacaatgt caaatccgag ctcaagaggg
     5221 cgttgaatcc agggggttgc aaaggtgtta tcggcaactg taatcaaatt atgttttttt
     5281 gcaatagcag cgatttttcg taaattagct agctttagca tgggattgga aggggtttcc
     5341 aaccatagta attttgtctt cggagttatt gcggcttcaa tattctcggg tactgacata
     5401 tcaataaaag aaaaggataa attagaagtt cgggttttta ctttatcaaa aagccggaaa
     5461 gttcctccat aaagatcatc catggcaaga acgtgatctc cggaatctaa taaatcaata
     5521 actgtattga ttgcagccat gcccgaagca aaagcaaatc ctttttggcc tgattcaagg
     5581 cttgctatgc aatcctcata ggcttttctg gtggggttct gggtgcgtga gtattcatag
     5641 ccaagatgtt cgccaggggc aatttgctta taggtggagg tagcataaat aggggtcatc
     5701 acggcccctg tactttgaca tggttcttgt cctgcatgaa ttgcacgtgt atcgaaatga
     5761 gtcttattca ttttttattc ccataaaaat atttaaattc aaagaaatac caataggcga
     5821 tcaaaataat gattaaaaat tatacttttg gcgccttttt tgctaaagtt catagtgtaa
     5881 atgccgatat ttttatagag tcattctaga gagggatgca tgcgtaggca agaaatcaat
     5941 tatgaaaaag ttgccaaggc cgcagaaaaa ataaaaaaaa gaggcataga gccgtccgtt
     6001 aatgaaattc gtgacgaact tggcttagtt gggaaccatc cgcaattatc aattttgctg
     6061 gaggagtggt atcacaatca accagaattt aaacgaaaaa gtcacacacc tttgactgaa
     6121 aatattaatt taaataccga tgaaattcgc gaaaaaaatg tagagcttga aaaatcaatt
     6181 tcgctgttac gggcaacact cgaatccact gcagatggta ttatgatggt taatggccat
     6241 ggagccgttg tcgattggaa tcaaaagttt gtagaaatgt ggcgaattcc ctcttatatg
     6301 atggagtcgg gaaaagaaag tatcagtttt gaatacatac ttgagcaact tattgatccg
     6361 caatctttga ttgctgatgt gcaatatctt tatcaaaatc tagagtggca aggtgaatta
     6421 cctgagcttc atttcaaaga cggtcggatt tatgagcgtt ttactcagcc tcagcgcgtg
     6481 ggctcacaaa tagtggggcg agtatatagt ttccgtgatg ttactcaaaa gaggatggct
     6541 ctcgatgagt tgagaatacg tgaacgtgct attgaagcca gcacacatgg tgtggtgatt
     6601 attgacgtaa caaaaaatga aaacaaagtc atttatgtca atcgagcttt cgaaagaata
     6661 acgggatatg gggagcaaca tgctttaggg aagggtttgc ttactttgct aggttcaaat
     6721 ttggaggagg taaaccataa acgtattgaa ctggcaatta gggaaagcaa agaagaaaca
     6781 attgaaatgg aaagtataaa acgaaatgga gagttctatt ggtgtgaaat cagcgttgct
     6841 ccggttaaag attcttttgg ctatgtaaaa cattatattt gcattttaaa tgatgtgact
     6901 caacgacgtg atatggaaga tcaactgttg cagcaagcaa cttatgattc cctgactaat
     6961 ttacctaacc gtgtcttact gatggataga gtggagcagg ccattctaca agcaagaaag
     7021 aacaaggcaa ttttggcttt tttatttttg gatttagatc ggtttaaact gactaatgat
     7081 actttgggac acagtatggg tgataaatta ttacaagcca ttgccaatcg attactcatt
     7141 gttacagagg attttgatac tgttgccaga ttggggggag atgagtttgt gatattactt
     7201 acagacatag ataatatgct ggaagctgaa acaattgcac aaaatatttt aaaaattata
     7261 gaaaaaccta ttcaaataga tcaacatagt ttgaagatta caggtagttt gggaatcagc
     7321 ttttatccca gagatggtga tgattacgaa tcattgatga aaagtgctga tttgtctatg
     7381 tatcatgcga aagatactgg aagaaacaac tatcgtgtct acgaacctga aatgaatagg
     7441 cgagttatta atcatatgca attagacaat gcgttaagag atgctttaaa aaatgatgaa
     7501 ttatttctgg tttatcaacc attgattgac ttaaagcaat ccagagtagt tggattcgag
     7561 gctttaatga gatggcatag caaaatattg ggacttgtta gcccggcgga ttttataccc
     7621 atggcagaag aaaatggcat gattttggaa atgggtgaat gggcaatgaa gcaagcttgc
     7681 atgcaagtaa aagaatggca taaagctggt tttaaaaact taagtattgc ggtgaatctt
     7741 tctggacggc agtttcgaca aaaaaatttg ccggaggttg tatcgcgagt attaaaaagc
     7801 tctggtttgc aatcgaggtt tttggaactc gaattaactg aaagcctgtt gatagaagat
     7861 attgatcacg ttgtagatac tatgtatgca ttgaaagaca tgggaaccaa gttagtgatt
     7921 gatgattttg gtacaggcta ctcaagcttg tcttatttaa aacaatttcc cgtagataaa
     7981 ctgaaaattg atcgttcgtt tatcactgaa atggtaagca atcagaatga cgcggctatt
     8041 gccaaagcga ttattaacct gggccacagt ttaaatcttc aggttcttgc ggagggtgtt
     8101 gaaaatgaat ttcaaagaga ctttataaca tctcatggat gtgactatgc tcaaggatat
     8161 ttttttaagg ctcctgatac tccagaaaat atattagaat ttttaaaaag tttatctgaa
     8221 tcgaaaataa agtaatgact aatttattaa gtgaagaatt cctgttttct ctcaaaactc
     8281 aaatcccatc ataaatctct ccagatcggt atgttttttg caagcaaagg ttaaattaat
     8341 caagtagttt ctgttggctt tgtttatttt atcttttatg ttgtttatat gaagggcaag
     8401 tatatagaac aactcaaaat gacaggttga gcctgatttt tacttttatt caagcaatat
     8461 attgattgaa tttgtgtatt ttttaaggag attcggaata gatgtcgtat tgatatagaa
     8521 tgggttggga ggataaatga agcacattac cataattgga gccgggttag cgggaacttt
     8581 atgtggttta tatcttgcta gaagaggcta tgaagtagag ttgtttgaat caagaccaga
     8641 cattcgtaac agtccgactg attatggccg ctccattaat cttgctttgt cgtgtagagg
     8701 aattaccgct ttaaaagcaa tgaacctgtt aagtgaagta aataaaatta tggttccaat
     8761 gcgggcaaga gctattcacg aagccaatgg cgaggttcac tatcaaccat ttggacgtca
     8821 cattgatgaa tatattaatg ctatttctcg ttctgatctc aatgccttgc ttttaaataa
     8881 agcggaatta tgccctaaca ttaaattaca ctttaatatg aaattgcact ctcttgatat
     8941 tcataataaa aaaattaaat ttgaaaataa aaatggtgat tttgttgagg tctcatacca
     9001 tagactaatt ggagcagatg gtgcgccgtc tcatgtaagg gatatgctga aaaatgaggg
     9061 aatagttagt gcaagcagag attttttatc tcatggatac aaggagcttt caattagcaa
     9121 aaaacataca aaagggatgg ctagagagca tttgcattta tggccacgtg attcgtttat
     9181 gctattaggt aaccctaatc ctgacgattc aattacaggt tctttgtttt tagctaatga
     9241 agggaaagac agctttgctg aactaaataa tgaagaaaaa ttacatttat tttttaaaac
     9301 acagttcccg gatgcctatg cagctatgcc aaatttggtt caagaatttt ttggtaatcc
     9361 tactggacat ttgagtacta tccaatgctc accctggtac tataaagacg aatgcttatt
     9421 aataggtgat gcggcacatg ggataattcc attctttggg caaggaatga atagcgcatt
     9481 tgaagattgc cgtattcttg atgaattact ggatgaatac caagacgact ggtcgcgagt
     9541 gaaccctgtt ttttatgaac acagaaaagt gaataccgat gcgatagcga aaatgtctat
     9601 ggataattat catgaaattc attcggacat aagaaatcca aaatttattt tacaaaagca
     9661 gattgaacgg gagctcatgt tgcgataccc ggaacattat gtttctatgc atgttttggt
     9721 tatgtttacc aatacccctt atgccaaggc gatggcaata ggcgagttac aatctggcct
     9781 tttagagcaa atttgtttcc caataactga tattaaggag ttaaattggc aggaagtaga
     9841 gaaattactt agcgtatatg acaaaaaatt ggcgaaaatt atttgattta atttatgtca
     9901 ttcattttaa atgaaattta tagtcaaagt tttgtcagaa aagatagatt cagggacatt
     9961 tttttgtcgc tacctgagga ttattagcgc aaagaataaa aaaacaacct aattttgtta
    10021 tattattgag cattttgatt aaaaagatgg cacgccttct gcagtataaa cttgtctaaa
    10081 taaagtttat atggagtctg ttatgaaaac tgttcaagcc tatattgatt caaaacaaca
    10141 tgagttcatg aatcatcctt tcttcaacat tttagaagaa ttaaatagta tagaagaaat
    10201 cagttatttt gtcccggagt taacattttg ggcaatgact tttcaagata tacttcgaat
    10261 taatgaagaa agagtaactg atccttattt aaagaaagta gctcgacatc acagattaga
    10321 agatgcagga cacgaaaaat ggtttttaag tgataaaaaa tacatgggtg aaatatgtga
    10381 taacaaggcc tgtgatcgcg atgatgttgc ctggctatac agtaaggatt cccaattggt
    10441 acgtgatgct gcctattcta ttatgtctga aatttataaa atagaggatg aaaatctgaa
    10501 tattgtcttg ctgttgaccc tggaatcatc aggacatgta ttttttgaga aagttgtcaa
    10561 acaagttaaa aagacgggtg aagataaaaa tctgaaatac ttctcaagtt ctcatcttga
    10621 agtagaatta gcacatgctt tatttgaaga ggaaatggaa agaaaagtat attccagaag
    10681 attaccaatt agcactcgta gagaagcact aaggatggta gatcgttgct atgatgcatt
    10741 cagcaagatg tttgatggat tgattcttgc ttgcaataaa cgattggaat tggcaaaaga
    10801 aaggaatcaa catgtcgcaa aatcagtgga gcttagagaa aatcaagcag tataaggaag
    10861 ataccggaca agcattgctc agtgatgagc aatcgcttgt tttatttggt caggattttg
    10921 gcaagctcat tcaatcacag ccggtagctg tgtccgcccc tcaaagcata gagtcattgc
    10981 aatcattaat tctattcgcc aatcaatatc acttaccagt aactattcgt ggtaatggtt
    11041 taagtcaagg cggccaatca ttgcctgttc ctggaggact aacaatttct atgcagtctt
    11101 tcaacaagcc acttgatttg agtgaagatc tcatctgggt tgaggccaat accagttgga
    11161 agaatctgct tgaaaaatca ctactgaaca ataaagcccc ctatgtgctg ccttataact
    11221 gtaatttgtc ggttgggggt gttttatctg caggaggaat aggggcttcc tcttttaaat
    11281 acggtgttat taatgcttat gtttcagcat tggaagtggt tgatggtttg gggagaaagc
    11341 aaatcgttga aaaaaactca cctctatttc aagcctgcct ttcagggcaa ggtcgatttg
    11401 gtgtcattac caaggcatgt attcagctac gatcagttca gccacgcgtc aaaactttct
    11461 ttctggttta tgctgatcag aatcaatggt ttgaagatat ttataaaata cagggtaaag
    11521 tcgattatat ggagatattt tgttctcctt caattcaggg cgctcgattg aaagaagata
    11581 aacgtattcc catggcttat tggttgtatg gtttgcacct ttctgttgaa tatgatcggc
    11641 atgcggaaaa tattttaggg cagttaaagc catggaatgt cctgaatata caggaagaga
    11701 gtattttatc ttatttttta aggcataact ctcgctttga tatgatgaag ttgacagggc
    11761 aatgggattt gttacatcct tggtatgaat gttttgttcc tacctcattt ttaactggag
    11821 tactttccca actgttagag gaattaccgc ttcactatgc cagtttggtt catgttgttc
    11881 caattgccaa gcaaaaagcc gggtttatga tgttaccaga ctgcgattca atttgtgaat
    11941 ttatgatttt aaatccaggg gtaccttatt ccctagagga aagttgcctg aaggctattg
    12001 agaatctcga caagcacttg ctgcaaaata atggtaaacg ctatttatca ggattcttag
    12061 gtacagaatt accagacaac tattggctaa agcattttgg agaaaaatac gactcatggg
    12121 taggtttgaa aaaacagtat gatcccgcag ggattttttc atcaatgtta catcatatat
    12181 aaaaaatagt attgtgcaaa ctcatgcttc gtaacagata tcaaggcatg atggtcgatc
    12241 tgatgcataa aggtatgaat tcagcctgga taaagatata ttatctacag ggcttacgaa
    12301 accccctatc tctttgttaa aaaaagtttt taattcggtc atttagttta ttctaagctc
    12361 cttcattaaa aacatttttt gccttgacct tgggatattt tgtaagctct gatctagtga
    12421 tcttgaagtg agggtgataa agatctattt tgtggtagga tgctatatat caatattatt
    12481 taaagagaag gatgaatttg ttttgatagg tttaactggg attggaaacg tatgaaattt
    12541 gatattcaat ttttgttttc agccaattac gctaaatgga taatcattag tttaatatcc
    12601 ttgtttggta ttttgattat ttttgaatgg atttccttat tttatccatt gacttatgtt
    12661 agggctatcc ctgtttcaaa tacaaaatct attgctggaa tgagagaaga gaatctcaat
    12721 tatattttca atacgtcctt atttggagtt tatgttcctg cagacttaaa tgaggataat
    12781 gtaaaacaat caatgctgaa tgttactttg gtcggtattc tttttgcgga caagatagaa
    12841 gagtctcaag tgattatacg atctgccagt ggggaagaaa aaacctataa tgtaggagat
    12901 aagatacctg gtggtgcaac aattaaacgt attatgccag gaggagtgct tgtggaaaga
    12961 gatggcactt tagaaagttt aagtcttccg aaaaatgatt taacctttga acctgtcgct
    13021 aagcctttaa aagaggaata aatggcatgc aacgcatctt taagttattc attctaataa
    13081 tttttattcc tatgatggct gcctgtattc atagtacctt aaatttacga gaaggaatcc
    13141 aatgctttaa agcacaggat taccggaagg cattcattcg tttaaaaccg gaagctgaaa
    13201 agggaaatcc agatgctcag tacgctgtag gttatatgta ttattatggt aaaggtgtgg
    13261 tagaagatag aaaaaaagcc cgtttctgga taacctgtgc tgcaagagca ggtcagccag
    13321 atgccatagt tgctcttgga attcttgatc gccaagcaag gcgggataga gaactatttc
    13381 aagataataa tttgcaacat tttcccgttg ataatggttt ataagttttt tactttaagc
    13441 taatttcttt gattcaaatg ctttcttaaa aacccgggta acccaggcca ggttagtttt
    13501 agctaactgt gtgcgttaat aatcaaagaa gccatgtaac aaaaatacac aagcaaaagt
    13561 atccccccag gccaggagga tgtctttttt tgataatagt aatttaaaaa caataaaagt
    13621 aaggtaatgg aaagcatcac tggcatatca cgccataata cgacgctgct aattttagtt
    13681 gggtttagta atcccggtag agctaagatt aacaacaggt tataaatatt tgagccaatt
    13741 atcgtaccta tcgctataga gtcttctcct ttgatagcgg caacaaggga cgttgtaagt
    13801 tctggcaagg aggaacctat agcaataatg gtcaagccaa tggtgaattc acttatacca
    13861 gcccattttg caatttcaga tgcattataa accaggtatt ttgaacaaat aggcagaatc
    13921 aataaaccta ttgcgatact caataaatta gagggtgttg aacgatagga tagtatcgcg
    13981 ggtctaaatt cattaactga taaatctctt ttaggcgagt gactagctaa ataaatgaaa
    14041 taacagagta ctgctataca tccgatcaaa aaaaggcaac catcaattgt acccaaataa
    14101 ccgtcaagta ttaagctgta cgcaaacagc atgacaatta tcaatattgg gtatactttt
    14161 tttagtttat tgaaatgaaa agaggttggt ttgataagga ttataatccc taaaattaag
    14221 ccaatatttg ctatatttga gccaatcgca tttccaattg ccaggtcatt tttatctttt
    14281 aaggaagaaa tgattgagat aaataattcg ggggcactgg tacccaatgc aacaatagtt
    14341 aaaccaatca taagggggga taattgaaaa cgaattgcca gccctgaggc accagcaacc
    14401 aaatgattgg cagcccaaag aagggcaata aaactgataa tcaaattcag aatagggttc
    14461 atttttgtac tcgatatttt ttagtgataa t
//
LOCUS       DBNCWK010000070        14389 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.070, whole genome shotgun sequence.
ACCESSION   DBNCWK010000070 DBNCWK010000000
VERSION     DBNCWK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14389
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.070"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            191..1216
                     /locus_tag="AC06IO_001848"
     CDS             191..1216
                     /locus_tag="AC06IO_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875027.1"
                     /translation="MGWTDRFWNAYRVVTNTVIEGAANSLNAAGSLACLLGGAGVSLS
                     YSMSETIKAGYFGSVGVAGTVNLDVTAVEFNYGFNQTIPFRRDLLEKSGGGSYALNDF
                     ANPGTIFMISSLAILSGTTLRTFGANLKKWQQNREDKYHFIAKYSTDLEPPSYKEYLY
                     VNAESFFNSLAIASFSSLLAKNVLDFSIKDLSKYHFTYPFNGTNITGSVKTPDYTGPV
                     TSEFFPLDFFVSQNTTIHTDYFNLIVNEKVKANAMTNVTYGGGVHLEPSHQSSAPLLA
                     ASATIGIGTYLAGTFFGKKILRERDERLQRSRSSSYSIVSGTFWDNKHDIEAKGDTKF
                     EHTHSLQ"
     gene            1279..2472
                     /gene="lapB"
                     /locus_tag="AC06IO_001849"
     CDS             1279..2472
                     /gene="lapB"
                     /locus_tag="AC06IO_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase LapB"
                     /protein_id="HIO9875028.1"
                     /translation="MVFKSRLVGIILLCFSSITGWCLSQSPEEDRIIVSNCLYEQLKD
                     KARLIAQSDHFSFIAIPIDENITNTLQKMRPVCGNYLNAEPYIQQTSNRFLDSQKLLD
                     KLTRYHPISTANPYPINHEAQVHGLFEQIDPAKIWQTNQHLTSYINRSAKSRTGVEAA
                     QWFKQQFDTLAQDYGRKDVESYFVKTGNKFIQPSVVTVIGKDKSGEALVIGAHIDTLD
                     GNMPGADDDSSGISVELEMARVLFSSNFELNRPVYFIAYAAEERGLIGSGYVVQDFLQ
                     KKIPVKAIMQLDQAGYRANAKDQTIWLLKDYVDKGLTEFTAELLTRYVKIPVGYTKCG
                     YACSDHVNWTNEGFKTTYPSATTLDDDNPYVHTSNDTLDILNLEHMVNFTKLGLAFIV
                     ELGLN"
     gene            2531..3421
                     /locus_tag="AC06IO_001850"
     CDS             2531..3421
                     /locus_tag="AC06IO_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875029.1"
                     /translation="MKTEALLSWLNVESNESYDSIRIKYQLRLEALEKEERIKKMGGI
                     NPFAPKTPLNNTRIITSKEIQEAWAAIDSLSKYQKFKGLVAQEENLSTIPNKKMDSKR
                     EETKLTHFVRERAQGPRGRRLPAHLQAKIKRVPLDKPHSKLITEGGEQQINAILDKTT
                     LSVSDLQSAVYTAVQNYTNYHMKSENKINPKYNRGQGDGFFSFFRHGSFGITTANNLY
                     ISISGKNATLDETIEKLKDFLGHSSRAYHHHSFTSYLADALAEKGLLTSHPASRYKQE
                     DVVNQLDQWIAQNSTVNLLK"
     gene            complement(3534..6464)
                     /locus_tag="AC06IO_001851"
     CDS             complement(3534..6464)
                     /locus_tag="AC06IO_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIO9875030.1"
                     /translation="MIDKMRKSKKPHPLNHIALGDNFNHFNLLERSLISQLIELSYDE
                     NTLHTLSLSGLKLLGKHPLLKDTITAVWHHDGSHINILASNGLKFDAPKELEKFLAVV
                     SDWHFLKIYKTKLKKHSEEKLKQRIDICLDKYQGNLFCISFWFDSIDCNFEILMPFFK
                     DILGLLNLSLAKLMNKKREKLLTTILEKAGDSVEITNEEAVIQYVNPAFENITLYNAR
                     EAMNKTVASLLRSPVEDAQLFEEINQTLKSGNTWKGQIRSRKKDGSYWVAQTIIVPVM
                     DDELGRITQHIAIKQDITEQVHRFNQLKISEERYRNLMNAVTDGIFIHNLQGAFLEVN
                     EAACLSLNYSREELINSYVWDIEVGFSQESLNQLWENVQKGPINTEGMHRRKDGTTFP
                     VDVRLAVFNATGEKLVLAIVRDITLQKRSEATIRKLTRALEQSPVLVIITDKTGTIEY
                     VNAKVIEQTGYRKEELVGGNTRILRSGQTPHEIYKTMWEHLANGEEWHGELLNKNKSG
                     EHFWVSAIISPIRNENEEITHYLAVMEDVTQKKSYEEMLKHQATFDNLTNLPNRLYAV
                     NKLERAISKSHINNQKLAVLFLDLDEFKQINDSLGHAIGDLLLKALSERFLSMIRQTD
                     TIARLGGDEFMMILENIMQDKDAEIIAKKCREICSQPFVIESQELLVSTSIGIAIYPE
                     HGKDAKTLMRNADTAMYQSKIRGKNNWTVFQNTMIEGANNHIRIKTELHQAFTRNELY
                     ICYQPIIELKENKVVAAEALLRWQNTTLGQINPDQIIALAEETGMIIPLGYWILDKVC
                     AQAKEWEFITGSKIKVAVNISTLQLKQKDFIHKVQLILKRTKLSADSLIFEVTESAFI
                     DDSKFILSQLNKLSDMGIHFSLDDFGVGYSFLQYIKSYPFKNLKIDRVFIQGVNTNHN
                     QLSLINSIITMARNLKLSVIAEGIETAEQLELMRSMNCDMVQGWYFSKPLSGDKFLFY
                     LMNK"
     gene            6687..7328
                     /gene="coq7"
                     /locus_tag="AC06IO_001852"
     CDS             6687..7328
                     /gene="coq7"
                     /locus_tag="AC06IO_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIO9875031.1"
                     /translation="MRTSSFLDRLIGEVDSALRTLVLPQKRITMRQSPAENLADTVLS
                     AQEKKHISGLMRVNHAGEVCAQALYQGQALTARLTHIKEQMASAAAEEVDHLAWCEER
                     LYELGSKPSLLNPIWYCGSVLLGALAGLAGDKVSLGFVAETERQVTAHLQRHLHYLPE
                     KDKKTIAILKRMQEDEEHHAHTAMEAGAVELPYIIKQLMNAVSKLMTQSSYYI"
     gene            7345..8340
                     /locus_tag="AC06IO_001853"
     CDS             7345..8340
                     /locus_tag="AC06IO_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIO9875032.1"
                     /translation="MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQ
                     AVLWAAFFNFIAFLIFNLTVAQTIGYGLIDTDLVDTYFILAALMGAIFWNLVTWYYGL
                     PSSSSHALIGGLAGAAIAKGGLSSLKITGFAKVAAGIFISPVLGLLIGLLFTHIFSLF
                     LRHKNEEELNTLFKSFQLISSALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPF
                     WVVISCHGVISLGTLAGGWRIVHTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPV
                     STTHTVTGSIAGVGLISGIGGAHWKVLRRIFYSWLLTIPAAALVAAAIMTISPTA"
     gene            complement(8350..9741)
                     /locus_tag="AC06IO_001854"
     CDS             complement(8350..9741)
                     /locus_tag="AC06IO_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9875033.1"
                     /translation="MDLFRKKDIKGSPDNESHLAKCLSVFDLTFLGVGAIIGAGIFVL
                     TGIVAATISGPAVIFSYVVAGFACAFAALSYAELAASIGGCGSAYGYAYAGFGELIAW
                     IVGWDLLLEYSIAVSAVSIGWSGYANDFLMALKIFIPTHLLHGPADGGDLNILAILII
                     AVLTTLLIVGVKSSSRFNNIMVLVKLLVIFIFIAIAFGEVKVENWSSFMPYGWAGVMK
                     GASIIFFAYVGFDAVSTAAEEAINPQRDLPIGIIASLAICTVIYILVSGLLTGIAHYT
                     TLNVSSPISHVLLVLGYKTAAGLVGVGAIAGLTTVMLVLFYGLTRVFLAMSRDGLLPR
                     FLSKTNEHTKTPIRIILLCGILMASLASVTPIGTLAELVNIGTLFAFIVVCGGVLYLR
                     RTHPDMERPFKTPFMPYVPILGIISCFYLILNLPWVTILRFIIWMLVGIAVYFAFSRT
                     NSALSVEGDCAAE"
     gene            9901..10461
                     /gene="pagP"
                     /locus_tag="AC06IO_001855"
     CDS             9901..10461
                     /gene="pagP"
                     /locus_tag="AC06IO_001855"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HIO9875034.1"
                     /translation="MKRLISCLTIICALNASAAAETTSSPCSRWISLLKPVCQRIHQT
                     WTEGHDDMYFSGYAWHNRYTYRPEKIKSYNEAAWGGGLGKSLFDEKGNWHGLYAIAFL
                     DSHRHIEPAVGYAYLKTASVNKDIKAGLGYSVLVTSRVDYDNVPFPGALPWVALFYKR
                     TTVAATYIPGSAGAGNVLYILGKISL"
     gene            10601..11026
                     /locus_tag="AC06IO_001856"
     CDS             10601..11026
                     /locus_tag="AC06IO_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HIO9875035.1"
                     /translation="MMLKTQLTAFIGAVILAGSSLANPIKPEVCPSVPSIQSEGMSMS
                     SEILEGMYITYNLSHYNTSSSWVFIVGPIAAENDDMALAESNKLLSTMSGSPHPEDDG
                     EGNWICQYTTKSKDIIAFAIEADDMLSPLKMMRYLRTIR"
     gene            11173..11715
                     /locus_tag="AC06IO_001857"
     CDS             11173..11715
                     /locus_tag="AC06IO_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HIO9875036.1"
                     /translation="MKPFIEQAQFYATYHQNQATRYTHMAGVPLIMLSIMIFLGFVKI
                     VIPGVYATDLACLATLAALIYYFLLNWQLALALTPILIFLLWLASWFNYDGPTKFGLW
                     VFIITFVVGWGLQFYGHFIEGKKPAFMVNFTQALIAPLYLTAELFFMAGLMNSLREQI
                     YGNEEVNTEEISKTKTKSKS"
     gene            complement(11785..13908)
                     /gene="rlmKL"
                     /locus_tag="AC06IO_001858"
     CDS             complement(11785..13908)
                     /gene="rlmKL"
                     /locus_tag="AC06IO_001858"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HIO9875037.1"
                     /translation="MNYSLFISCAKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPV
                     IYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQ
                     IRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
                     HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAH
                     IAPGLLRQDQSLQYWARHQSSLWEKLRTQALQQVKPLAVKLVGTDTDSKVVALARSNA
                     ERAGVLPLVEFNTLSLNACRPGTKRGLVVCNPPYGERLGEVTQLVPLYQQLGTTLHTC
                     YQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLS
                     DNAQMLFNRLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPP
                     ASIPVHKAEKRSLEMLQVVPRALGIHPEKLIVKQRKQQKGSEQYQKIGKTSQRQIVTE
                     GKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEPGTRFLNCFCYTASASVHAALAGALT
                     TNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDCKEWMKTTRDKFDVIFLDPPSFSNS
                     KRMSDILDIQRDHVSLINMAMRLLNPDGVLYFSTNLRQFKLEPMLKEKYAVQDITTQT
                     IDQDFKRNSKIHHCFKIVMPHFADN"
     gene            complement(13905..14384)
                     /gene="yjgA"
                     /locus_tag="AC06IO_001859"
     CDS             complement(13905..14384)
                     /gene="yjgA"
                     /locus_tag="AC06IO_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIO9875038.1"
                     /translation="MDEQISKSQKKRDAHYLQDIGIKFIDLSASKLDSLPLPDNLRQA
                     IIDARSIKSHGAKRRQAQLIGKLMRAADFEAILAAYEKILEEESSITASFHEIELWRD
                     RLLQDGKDALTEFIDAYQPEDVQQLRQLIKKALDDQMKEKNTGAAKALFRYLRSLIK"
BASE COUNT         4454 a         2984 c         2613 g         4338 t
ORIGIN      
        1 gtcaacgtga aaaccattcc acaatgacca aaacaaaacc acaagtgggc tatttttcca
       61 ctaaatgcta ttttttgcca caaataaatc tggcttttaa aaaaaatatg ttattctttc
      121 caacatcact gattcgtact taatcaaagt ggcgaattta ttttttatca tttaacaaaa
      181 gactttaaat atgggatgga ctgatcgatt ttggaatgcg tatcgcgtgg ttactaatac
      241 cgtaatcgaa ggcgcagcaa actctttaaa tgcagctgga tcactagctt gcctgctcgg
      301 aggcgctggg gtctctctct cttactccat gtctgaaaca ataaaagcag ggtacttcgg
      361 atcagttggc gtagcaggca cagttaattt ggatgtgact gcagtagaat ttaattatgg
      421 atttaatcaa accattcctt ttcgcagaga tttgctggaa aaatccggcg gtggcagcta
      481 tgcattgaat gattttgcca atccaggtac tatttttatg attagttctc tggctattct
      541 ttccggaacc actttaagaa cttttggggc taatcttaaa aaatggcaac aaaacagaga
      601 agacaaatac cactttattg ccaagtacag cactgacctg gaaccccctt cttacaaaga
      661 atatctgtat gtcaacgcag aatccttctt caattctttg gcaatagcct cattcagcag
      721 tcttttggca aaaaatgttc tggatttttc aattaaggat ttatcgaaat atcatttcac
      781 ttatcctttt aatggaacaa atataactgg ctcagtgaaa acgcctgatt atactgggcc
      841 agttacatca gaatttttcc cccttgactt ttttgtgtct caaaatacaa ctatccacac
      901 agattatttc aacctgatag tcaatgaaaa agtgaaggct aatgcaatga ccaatgtcac
      961 ttatggtgga ggagtacatt tagagcccag ccaccaatca agcgcccccc ttctggctgc
     1021 ttctgcaacc attggtatcg gtacctattt ggcaggcact ttttttggca agaaaatcct
     1081 gcgagaacgt gatgaaagac ttcaacgttc aagatcatcg agctattcca tagtttcagg
     1141 cactttttgg gataataaac atgacataga ggcaaaaggc gatacaaaat ttgaacatac
     1201 ccattcatta cagtaaagaa tagcctaagt ctcctccatg taatatattg caatgaaaaa
     1261 aataaaacgg agtttttcat ggtatttaaa tcaaggttag ttggcattat tttattgtgc
     1321 ttttcaagta ttaccggatg gtgtctttct cagtcccctg aagaggatag aatcattgtt
     1381 tcaaactgcc tgtatgagca attaaaagac aaagccaggt taattgcaca atcggatcac
     1441 tttagcttca ttgccattcc aattgatgaa aatattacca atactctaca aaaaatgaga
     1501 ccagtttgtg gaaattatct taatgctgag ccatatatcc aacaaacctc caatcgtttt
     1561 ctcgattcgc aaaaattatt ggataaactg acccgctacc accccatctc gaccgcaaac
     1621 ccatacccga ttaatcatga agcacaagtc catggtttgt tcgaacaaat tgacccggcc
     1681 aaaatatggc aaaccaatca gcatttaacc agttacatca accgctctgc caaatccaga
     1741 acgggagtag aggctgcaca atggtttaaa cagcaatttg ataccttagc acaggattat
     1801 ggaagaaaag atgtagagag ctacttcgtc aaaaccggaa ataaatttat acaaccctcc
     1861 gtcgtcactg taattggaaa agataaatcg ggagaggccc ttgttatagg ggcacatatt
     1921 gacacactgg acggcaatat gcctggtgcc gatgatgact ctagcggaat atccgttgaa
     1981 cttgaaatgg caagagtgct cttttcatcg aattttgaac tcaatagacc agtttatttt
     2041 atagcttatg ctgctgaaga aagaggactt atcggctctg gatatgtggt acaagatttt
     2101 ttacagaaaa agattccggt gaaagcaata atgcagttag accaggcagg atacagagct
     2161 aacgccaaag accaaacgat ttggctgtta aaagattatg tagataaggg tttaactgaa
     2221 tttacagcag aattattaac tcgctatgtc aaaattcctg ttggctatac caagtgcggt
     2281 tatgcatgca gcgatcatgt gaattggacc aatgaaggct ttaaaaccac ctatccgtct
     2341 gcaactactt tagacgatga taatccttat gttcatacct ccaatgatac tttggatatt
     2401 ctgaatctgg aacatatggt taatttcact aaattaggcc tggcctttat agtagagctt
     2461 ggtttaaatt agcgatgaaa gcaatagagc tcgtgccaat ttaacggtgc tcagcaattg
     2521 gagtttatta atgaaaacag aagccttact tagctggcta aatgtagaaa gcaatgaatc
     2581 ctatgattct atcagaataa agtatcaact aagacttgaa gccttagaga aagaagagcg
     2641 tattaaaaaa atgggaggta tcaatccttt tgcgcctaaa acacccctta acaacaccag
     2701 gataatcacc tccaaagaaa tacaagaggc atgggctgct attgattctc tcagcaagta
     2761 ccaaaaattc aaaggactag tggctcaaga agaaaattta tccactatcc ctaacaaaaa
     2821 aatggatagc aagagagagg aaacaaaatt aacccacttt gtaagagaac gtgcacaagg
     2881 ccctcgggga agacgcttgc ccgcccattt acaagctaaa attaaaagag tccctttgga
     2941 caaaccacac agtaagctaa ttactgaagg gggcgaacaa caaattaatg caattttaga
     3001 caaaaccacc ttgtcagtga gtgacttgca atctgctgtt tacaccgcgg tccagaatta
     3061 tacgaattat cacatgaaat ccgaaaataa aattaatccc aaatacaaca gaggacaagg
     3121 tgatggtttt ttcagttttt tccgccatgg aagctttgga atcactacag cgaataacct
     3181 atatatttcc atcagcggaa aaaatgctac tttagatgaa accatagaaa aattaaaaga
     3241 ttttttaggc cattcctcca gagcatatca tcatcattct ttcacatctt acctggctga
     3301 tgcacttgct gagaaggggc tattaacatc ccatcctgcc agccggtata aacaagaaga
     3361 cgtagtgaac cagcttgacc aatggatagc acaaaactca actgttaatc ttttaaaatg
     3421 ataatggcaa ggatagtctg tttttttact cctgaagtaa aaacaaataa gttagagtcc
     3481 gccaccgaca tccgtgccaa atctttaaat taggcacggc agctggtaca tttttactta
     3541 ttcattaaat aaaataaaaa tttatccccg ctcaaaggct tggaaaaata ccatccttgt
     3601 accatatcgc agttcattga gcgcattaac tccagctgtt ctgccgtttc tattccttca
     3661 gcaataacag ataatttcag attgcgcgcc atagttataa tactattaat caaacttaac
     3721 tgattatgat ttgtatttac accctggata aaaacgcgat caatttttaa atttttgaat
     3781 ggataggatt taatatactg caagaaagaa taacccaccc caaaatcatc caaagaaaaa
     3841 tgaatgccca tgtcacttaa tttgtttaat tgagataaaa tgaattttga atcatcaata
     3901 aatgccgatt ctgtaacttc aaaaattaga gaatctgcag ataattttgt tcttttcaaa
     3961 atgagttgca ccttatgaat aaaatccttt tgctttaatt gtaaggtaga gatattcacc
     4021 gctactttta tcttgctacc ggtaataaat tcccactctt tcgcttgcgc acacacctta
     4081 tccaaaatcc aatatcccaa tggaataatc ataccggtct cctcggccaa ggcaataatc
     4141 tgatcaggat taatttgacc taaggttgta ttctgccagc gtaacaaggc ctccgcagcc
     4201 acgaccttat tttccttcag ctcaatgatc ggttggtagc aaatatataa ctcattacgg
     4261 gtaaacgcct gatgtaattc cgtcttaata cgaatatggt tatttgcccc ctcaatcata
     4321 gtattttgaa ataccgtcca attatttttg ccccgtatct tgctctgata catagctgta
     4381 tctgcattac gcattaacgt tttggcgtcc ttgccatgct caggatatat tgcaattcct
     4441 atacttgtag acaccaatag ctcttgtgat tcaataacaa aaggctgaga acaaatttcc
     4501 cggcactttt ttgcgataat ttcagcatct ttatcctgca tgatgttttc taaaatcatc
     4561 ataaattcat caccgcctaa gcgggcaata gtatcggttt gccgtatcat agatagaaag
     4621 cgctccgata acgcttttaa taataaatct ccaatcgcat gccctaagga atcattaatc
     4681 tgtttaaatt catccaaatc taaaaacagc accgccaatt tttgattatt gatgtgggat
     4741 tttgaaatag ctcgttccaa tttattaact gcatagagac gattgggaag gttggttaaa
     4801 ttatcaaatg ttgcctggtg ttttaacatt tcctcgtagc ttttcttctg agttacatcc
     4861 tccataacgg ccagataatg agttatctct tcattctcat tacgaattgg agatataatt
     4921 gctgataccc aaaaatgctc tccggatttg tttttattca gcaactcacc atgccactct
     4981 tccccattgg ctaagtgttc ccacatggtt ttataaattt catgaggagt ttggcctgat
     5041 cgtaaaattc ttgtatttcc accaaccaat tcttctttgc gataacctgt ttgctctatc
     5101 acttttgcat tgacatactc aatggttcca gttttatcag ttattatgac taaaacaggg
     5161 ctttgctcta acgctcgtgt taattttctt atagttgctt cagagcgttt ttgaagtgta
     5221 atatcccgta ctatagccaa aaccaatttc tccccagtcg cattaaaaac tgccaaacga
     5281 acgtcaacag gaaaagttgt gccatctttt ctgcggtgca tgccttctgt attaatgggg
     5341 cccttttgaa cattttccca caactgattt aaactttcct gagaaaatcc cacttcaata
     5401 tcccaaacat aggaattaat taattcttcc cgactataat tcaaagacaa acaagctgct
     5461 tcattaactt ctaaaaatgc tccctgcaaa ttatggataa aaataccatc cgttacagca
     5521 ttcatcagat ttcgatatcg ttcttcactg atttttagtt gattgaatcg gtgtacttgt
     5581 tccgtaatgt cttgtttaat tgctatatgt tgggtaattc gccccaattc atcatccatc
     5641 acagggacga tgatggtttg agcgacccaa tagctgccat ccttttttct tgagcgtatt
     5701 tgccccttcc atgtattccc cgatttaagg gtttggttta tttcttcaaa caactgagcg
     5761 tcttcgaccg gtgagcgcaa taaggacgcc actgttttat tcattgcttc acgggcatta
     5821 tacaaagtga tgttctcaaa agcaggatta acatattgaa tgacagcctc ttcattcgtt
     5881 atttcgaccg agtcgccagc tttttccaaa atagtggtca gcagcttttc ccttttttta
     5941 ttcatcaatt tggctaaaga caggtttaat aaacccagaa tatctttaaa aaaaggcatt
     6001 agtatttcaa agttacaatc aatagaatca aaccaaaaac taatacaaaa taaattacct
     6061 tgatatttat caaggcaaat atcgatacgc tgctttaatt tttcttcaga atgcttcttg
     6121 agctttgtct tatatatttt taaaaaatgc caatccgaga ctacagctaa aaatttctcc
     6181 aattcctttg gcgcatcaaa ttttaatcca ttagatgcca gaatattaat atgactgcca
     6241 tcatgatgcc aaactgcggt gattgtgtct tttaataagg gatgcttgcc aagtaatttc
     6301 aacccactta agcttaaagt atgtagggta ttttcatcat aactcaattc aatcaattgg
     6361 cttattaagc ttcgttctag caggttaaaa tggttgaaat tatctcctaa agcaatatga
     6421 tttaatggat gcggcttttt actcttcctc attttatcta tcatgtgcca atttagacct
     6481 acacctatca tttctgtata ataaagctta ggatcttatt gtgtaataat caaaatagat
     6541 gtataaaatt taccctgcca acaactccat cttaactcgc agccaccgat attaaatagc
     6601 cttataaaat ttaattgacc aatgacttca aactacacac taatcaagtt aacatagaga
     6661 ccttacactt tatttttaaa gtatccatgc gaacatcaag cttcctcgat agattaatag
     6721 gtgaagtcga tagtgcgctt cgaaccctcg ttttgccaca aaaacgtatt acaatgcgcc
     6781 aatctcccgc tgaaaatctg gctgatactg tgctgtccgc tcaggaaaaa aaacatattt
     6841 caggattaat gagagtaaat catgccggag aagtatgtgc tcaagcgctt tatcaaggac
     6901 aagctttaac agcacgactt actcacatca aagagcaaat ggctagtgcc gcagctgaag
     6961 aagttgatca tttggcctgg tgcgaagaac gtctgtatga attaggcagc aaacccagcc
     7021 ttttaaatcc catctggtat tgtggttcgg ttctattagg cgccctggct ggattggctg
     7081 gcgataaagt cagcttggga tttgttgctg aaacagagcg ccaggtaacc gctcatttac
     7141 aaaggcatct gcattatctc ccggaaaagg ataaaaaaac tatagccata ttaaaaagaa
     7201 tgcaagaaga cgaggaacat catgcgcata cagcgatgga agctggagca gttgaacttc
     7261 cctatattat taagcaattg atgaatgctg tatcaaaatt aatgactcag agcagttatt
     7321 atatttaaca ggatatgatt acatatggac tacaatacga tattcattct attcgtcata
     7381 tttattgcgt tcgtatttga ttttatcaac ggatttcatg atgccgccaa ttcgattgca
     7441 accatagtca ctacaggtgt tttgacgcca agacaagctg tactatgggc tgcttttttt
     7501 aacttcattg cttttctgat tttcaattta accgtagccc aaacaatagg ctacggacta
     7561 atcgatacgg atctggtgga tacttatttt attttagcag ccctgatggg agcgatattc
     7621 tggaatttgg tgacttggta ttatggactg ccatcaagct cctctcatgc cttaattggc
     7681 ggattagctg gtgcggcaat agccaaggga ggtctgtctt cactcaagat tacaggtttt
     7741 gccaaagtag cggcagggat atttatatct cccgttctgg gattattgat tggcttatta
     7801 tttactcata ttttctcttt gtttctcaga cataagaacg aagaggaact gaatacatta
     7861 tttaaaagtt ttcaactgat atcttcagca ttattgagta tcacacatgg aggaaatgac
     7921 gcacagaaaa cgatggggat cattgcggta ttactttttt ccgcatcttg gcttgatggt
     7981 gtattttatg tgccattttg ggtcgtcatt tcatgtcatg gggtaattag tttggggact
     8041 ttggctggtg gctggaggat tgtgcatacc atgggaacta aaattaccaa acttaacacc
     8101 ctgaagggtt gtgctgctga aacaggagca gccattacca ttttcgctgc aacagaattt
     8161 ggagtacctg tctcaaccac ccatacggtc accggctcca ttgcgggagt tgggctaatc
     8221 agtggcatag gtggcgccca ctggaaagtt ttacgcagga tattctactc ttggttgctt
     8281 accattccag cagcagcatt agttgctgcc gctattatga ccatctcacc aactgcctag
     8341 attatggcgt cactcggcag cacaatctcc ctcaacactt aaggcactat ttgtcctgct
     8401 aaatgcaaaa tagaccgcta taccaactag catccaaatt ataaaacgca atatcgttac
     8461 ccatggcaaa ttcaaaatta aataaaagca gcttattata cctaatatag gaacataagg
     8521 cataaaaggt gttttgaatg gcctttccat atcaggatga gttcgcctta aatataaaac
     8581 acccccacaa acaacaataa aggcaaacaa ggttccaata ttaaccagct cggccaaggt
     8641 accaattgga gttactgaag cgagagatgc cattaatatg ccacatagca atataattcg
     8701 aattggtgtt ttagtgtgtt cattcgtttt ggataagaac cttggcaata agccatccct
     8761 tgacattgct aaaaatactc tggttaatcc atagaacagg actaacataa ctgtggttaa
     8821 accagcaatt gcgcctacac cgaccagtcc ggctgctgtt ttataaccca agacaagcag
     8881 gacatgactg attggtgatg aaacgttaag ggtagtgtaa tgagcaatcc cggttaataa
     8941 acctgaaacc agaatataga tcactgtaca aatagccaga gaagcgatta tccctatggg
     9001 taagtcccgt tgaggattaa ttgcttcttc agcggcagta gatacagcat caaaccccac
     9061 gtaggcaaaa aagatgattg aagcaccttt cataacacca gcccaaccat agggcatgaa
     9121 agaagaccaa ttttcaacct tcacttcacc aaatgcgata gctatgaaaa taaaaataac
     9181 caaaagctta acaagcacca tgatattatt aaatcgagaa ctggatttca caccaacaat
     9241 taataatgta gttaaaacag ctatgatcaa aatagctaaa atattgagat ccccaccgtc
     9301 agcgggacca tgtaataaat gagttggaat aaatattttc agagccatta aaaaatcatt
     9361 ggcataacca ctccagccga tagaaacagc cgatacagca atagaatatt ccaataacaa
     9421 atcccatccg acgatccacg cgattaactc accaaaacct gcgtaagcat atccataagc
     9481 actgccacaa ccccctatgc tggcagccaa ctcagcatag gataaggcgg caaaggcgca
     9541 ggcaaatccg gcaacaacat aggagaatat cacggctggt ccgcttatag tggcagcaac
     9601 tatgcctgta agaacgaaaa taccagcacc tataatcgcg cccactccca aaaaggtaag
     9661 gtcaaaaaca ctaaggcatt tagctaaatg tgattcattg tctggcgacc cttttatatc
     9721 ttttttacga aacaagtcca tgatagtatg ctctcaggtt tattttttat tgtttatttc
     9781 caaaatttaa gttatcaata aacataaact ccggttatct tccatctaac aaaaccttac
     9841 acaattaaac tctaccatta aattatagct gggagaaacc ctgcaaaggt taaaaattga
     9901 atgaaaagat taatttcctg cttaaccatt atttgcgctt taaatgcgtc cgctgcagcg
     9961 gaaacgacat ccagtccttg ttctcgctgg ataagtttgc ttaagcccgt ctgtcaacgc
    10021 atccaccaaa catggacaga aggccatgat gatatgtatt tttcaggcta tgcctggcat
    10081 aaccgctata cttacaggcc tgagaaaatt aaatcctaca atgaggccgc atggggtggt
    10141 ggattgggta aaagtctttt tgacgaaaag ggaaactggc atggccttta tgccattgcc
    10201 ttcctggact ctcatcgcca tatagagcca gccgttggtt atgcttatct taaaacagct
    10261 tccgtaaaca aagacataaa ggctggcctt ggatactctg tattggttac ctctcgcgtt
    10321 gattatgata acgtaccttt tcccggagca ttgccatggg tcgccctttt ttataaaagg
    10381 acaacagttg cagcaaccta catccctggg tcggcaggag caggaaatgt attgtatatc
    10441 cttggaaaaa tatccttata aatatgaatt agccattgca tatggcaaaa gataatctga
    10501 aacaattccc gacaacatcc ttttaaaacg gttgcaactg aaaattcaac ttgttagtct
    10561 tttgaagatt tttaactgct aagtaacaag gagaagtaac atgatgttga aaacccagtt
    10621 gactgctttt atcggtgctg taatcttggc tggctcttct ttagcaaatc caataaaacc
    10681 tgaggtatgc cccagtgtac cctctattca atcggaagga atgtccatgt cttctgaaat
    10741 tttggagggc atgtacatca cctataattt aagtcattac aataccagtt caagctgggt
    10801 gtttattgta gggccaatcg cagctgaaaa tgatgatatg gcattggcag aaagcaataa
    10861 attactttca accatgtcag ggtctcccca tccggaagat gatggagaag gcaattggat
    10921 atgtcagtat acgaccaaat ccaaagatat tattgcattt gccatagaag cagatgatat
    10981 gctttctcca ttgaaaatga tgagatatct cagaacaatc cgctgataag tggataacac
    11041 ctgcacggac acggaagcat gatttgcttc cgtgatttaa cgaatagtta agagctcttc
    11101 ctctcttttt ggtacttctt tattttatgt gctatgtttt cttcatgacc ctaacttcag
    11161 gacgaaaaga aaatgaaacc gtttattgag caagctcaat tctatgcaac ctaccaccaa
    11221 aaccaggcga cacgttatac acatatggca ggagtcccgt taatcatgct ttctatcatg
    11281 atttttctgg gatttgttaa aatagttatt ccaggtgttt atgcaaccga tcttgcttgt
    11341 ttggctacgc ttgccgccct catttattac ttcctgttaa actggcaact tgccttggca
    11401 ttaactccca ttttgatctt tttgttatgg ctggccagtt ggtttaacta tgatggccca
    11461 acgaaattcg gactttgggt atttattatt acttttgttg taggctgggg attgcaattt
    11521 tacggtcatt ttattgaagg aaaaaaacct gcctttatgg ttaactttac ccaagccctt
    11581 attgcgcctt tgtatttaac agcggaattg ttctttatgg caggactcat gaattccctg
    11641 agagaacaaa tttacgggaa cgaagaagta aatactgaag aaataagcaa aaccaaaaca
    11701 aaatccaaat cttaaagaga gtcgagcttc acgcttatga aagggaagct cggcaagaga
    11761 tctaataata aataactgaa ttattcagtt atccgcaaaa tgcggcatta caattttaaa
    11821 acaatggtga attttgctat ttcgtttaaa atcctgatcg atggtctgcg ttgtaatgtc
    11881 ttgtactgca tatttctctt ttagcattgg ctctaactta aattggcgca gattagttga
    11941 gaaatataaa acaccatcag gatttaacaa ccgcattgcc atgttaatca aagaaacatg
    12001 atctctttga atgtctaaaa tatcgctcat acgctttgag tttgaaaagc tggggggatc
    12061 caaaaaaata acatcaaatt tatccctggt agttttcatc cattccttac aatcgtattg
    12121 caaaaattga tgcttggata aattaatatc atttaatcta aaattatctt ccgcccaaag
    12181 caaatacgtt ttggataaat caacattcgt ggttaatgca ccggccaagg cagcatggac
    12241 gcttgcactg gcggtataac aaaaacagtt caaaaatcga gttcccggct ctaattgagc
    12301 aaatttcaaa cgcataagtc tatgatccag aaatagtcct gtgtcaagat aatcatacaa
    12361 attcactatc aattttgcct taccttctgt cactatctgt ctttggctgg ttttacctat
    12421 tttttgatat tgttctgagc ccttttgctg ctttctttgt ttaactatta atttttcagg
    12481 gtgaatacct aaggcccgtg gaacgacttg caacatctca aggctgcgtt tttctgcctt
    12541 gtgtactgga atgctggctg gtggcgcata ttcttgcagt acagcatagt cattatatat
    12601 gtctatggca taggcatatt cgggtaaatc cgcatcgtaa attcgataac aggtgatttg
    12661 attttttctt gcccatttct gtaaatgatt tctgtttttt tccaatcggt taaatagcat
    12721 ttgtgcatta tcagaaagcg gggcatttgg ggtatttttt agtttatttg cagcagaaag
    12781 agtcaaacaa tagagtttgc actctaatgg gccattatat aaagtatatt gcttatcagc
    12841 acgtaatcct aatgctttag ccaaaactgg actggaagtt aaaatagccg cttgccagcc
    12901 ttgataacaa gtatgtaatg tagtacccaa ttgttgatat aaaggaacaa gttgagttac
    12961 ctcccccaat cgctcaccgt aaggtggatt acaaacaact aatcctcttt ttgtccctgg
    13021 cctacaagca tttagcgata atgtattaaa ttcaaccaaa ggtaatacgc ctgctctctc
    13081 agcattggaa cgcgcaagag caactacttt gctatccgtg tccgtaccaa ccagcttaac
    13141 tgccagtggt ttaacttgct gtaatgcctg agttcgtaat ttttcccata aagaactctg
    13201 atgtcgggcc caatattgta aggattgatc ttgccgcaat aagcctggag caatatgagc
    13261 cgccatcatg gcagcttcga taactaatgt gcctgaaccg caaaacggat catgtaaacc
    13321 atatccttga gcggccaact ctggccattt cgctcttaat agcatggctg ctgcaacgtt
    13381 ttcctttaaa ggagccttcc ctgctttttt acgatatcct ctttgatgca ggctatatcc
    13441 aaccaaatca aagctcaccg ttaaaatatc atttttcaaa tgagcatgaa ttaaaatttg
    13501 cggtttttct ttgtcgacag aaggccttga accattcaag cgtcgaaaat gatccactat
    13561 tccatctttg actatctgag cgccaaacat ggtattacga atttgctcag aggcaccatg
    13621 aaattcaatg gctatggttt tatcatgggt gaaaaccgtt tgccaatgaa agtctgtgca
    13681 taactgatgt acggcttgtt cttttgcagc atggccacta aataaaataa gctgtattct
    13741 gttagcaagc cttgaccata aacataaatt ataaattaca ggcaggctag cttcaccata
    13801 gacaccttga ggactcactt gagtcacatg aagccccaat cccttgagtt cgtcttccag
    13861 caaatactcc aaaccttttg cacaactaat gaatagagag taattcattt tattaaagac
    13921 cttaaatagc gaaacagtgc tttagcagca cccgtatttt tctctttcat ctgatcatct
    13981 aatgcctttt taatcagttg ccttaattgt tgcacgtcct caggttgata agcatcgata
    14041 aactctgtca aagcgtcttt tccatcttgt aataacctgt cgcgccatag ctctatctca
    14101 tgaaaagagg ccgtaattga actctcttct tcaaggattt tctcataggc agcaagaatg
    14161 gcttcaaaat cggctgcacg cattaacttg cctattaatt gcgcctgtcg ccttttagcc
    14221 ccatggcttt tgattgacct ggcgtcaata attgcttgac gtaaattatc gggaaggggc
    14281 aaagaatcca gtttggatgc gcttaaatca ataaatttaa tgcctatatc ttgtaaataa
    14341 tgagcatctc tttttttctg tgatttactg atttgttcat ccatagggc
//
LOCUS       DBNCWK010000071        14322 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.071, whole genome shotgun sequence.
ACCESSION   DBNCWK010000071 DBNCWK010000000
VERSION     DBNCWK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14322
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.071"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(77..153)
                     /locus_tag="AC06IO_001860"
     tRNA            complement(77..153)
                     /locus_tag="AC06IO_001860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Ile,seq:cat)
     gene            complement(242..2107)
                     /gene="rpoD"
                     /locus_tag="AC06IO_001861"
     CDS             complement(242..2107)
                     /gene="rpoD"
                     /locus_tag="AC06IO_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIO9875039.1"
                     /translation="MTMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHF
                     DVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVR
                     MYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIR
                     LNEIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPE
                     QAKIYFDELREAFEKAMKALKEHGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHF
                     RQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLGWLETIISKHAKKLDLQR
                     IEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLV
                     ISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMIETINKLNRISRQILQETGREATPEELAEKMELSEDKIRKVLKIAKE
                     PISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREAKVLRM
                     RFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG"
     gene            complement(2194..3927)
                     /gene="dnaG"
                     /locus_tag="AC06IO_001862"
     CDS             complement(2194..3927)
                     /gene="dnaG"
                     /locus_tag="AC06IO_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIO9875040.1"
                     /translation="MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHN
                     EKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGT
                     GEKHKSSLNLYNLMSEVSQYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGW
                     HNLEKAFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKD
                     QKPKYLNSPETVLFQKSRELYGLHQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVA
                     TLGTATSTFHIQLLAKHTKHLIFCFDGDSAGKQAAWRALESSLPHLNAGLDAGFIFLP
                     NEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYL
                     QKMVEGSYKQLLIDDLSRLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLL
                     QNPEIYSIAIQHINPALLHEQEHHILLKLLQQLKDKPNANTATLIEFWRNSSYFELIV
                     KLAAWDHQVPEQELTKEFIDVLLFLQKQNREILIQQYIEKSRKTGLTEAERLSLQNLL
                     KERHGQAEIEK"
     gene            complement(3936..4379)
                     /locus_tag="AC06IO_001863"
     CDS             complement(3936..4379)
                     /locus_tag="AC06IO_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2359 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875041.1"
                     /translation="MTIKEQINNDIKDAMRAKDKNLLNALRLISAAVKQIEVDERIEV
                     DDERMLVILDKMSKQRKESIAQFEKASRDDLVAQEQFELNVISKYLPEPLTDNEIEEL
                     ISDAIKSTGAEKMSDMGKVMAILKPKLQGRADMAQVSTRIKAKLS"
     gene            complement(4582..4821)
                     /gene="rpsU"
                     /locus_tag="AC06IO_001864"
     CDS             complement(4582..4821)
                     /gene="rpsU"
                     /locus_tag="AC06IO_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIO9875042.1"
                     /translation="MPTVRVKEGENPEYALRRFKRSCEKAGILTELRRREFYEKPTAE
                     RKRKQAAAVKRHLKKISRDVSSRRGVGHRRKKSTT"
     gene            5026..6027
                     /gene="tsaD"
                     /locus_tag="AC06IO_001865"
     CDS             5026..6027
                     /gene="tsaD"
                     /locus_tag="AC06IO_001865"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIO9875043.1"
                     /translation="MLVLGIESSCDETGVAVYDSESGLLSHALHSQIATHRVHGGVVP
                     ELASRDHVNYLVPLVDEVLTKAQIRKNQLDGIAYTAGPGLIGALLVGSCFAKSLAYAL
                     NIPALAIHHLEAHLLAAKMETPSLDFPFIALLVSGGHCQLIEVNNIGEYRLLGDTLDD
                     AVGEAFDKTAKLMGIPYPGGAVLANLADQCLSTPYQFPRPMTDRPGLDFSFSGLKTHA
                     LNTWNQSAKKEGDRSEIAKAFQQAVVETLIIKCKRAIKESQSKRLVVAGGVGANKALR
                     SALQKWIKDIKGEVYFPALEYCTDNGAMVAYAGCLRMMRGESDGGLGVMVKPRWPLA"
     gene            complement(6202..7035)
                     /gene="plsY"
                     /locus_tag="AC06IO_001866"
     CDS             complement(6202..7035)
                     /gene="plsY"
                     /locus_tag="AC06IO_001866"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIO9875044.1"
                     /translation="MALFIFLILVGYLMGSINSAIIVCRTFGLPDPREEGSKNPGATN
                     VLRLGGKQYGIMVMVFDALKGILPVILAKLLSAEPVTVAFTALAAVVGHMYPVFFHFR
                     GGKGVATTIGALLAFHFVIGVMVAATWLLVANFWRYSSLASIASISLAPFYSLILVGN
                     LNIFPPLFMITILVLYKHRDNFNRLIDGKEPKIKFKHSVIEEIMEASPATSAEQEFPG
                     KEVIDTNIDEAEKTEQAEAVKKPKAKKATTKAKKTTSKEETAKKPKSTKPKTKTVKEK
                     E"
     gene            7389..8798
                     /locus_tag="AC06IO_001867"
     CDS             7389..8798
                     /locus_tag="AC06IO_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIO9875045.1"
                     /translation="MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINAL
                     TERIPPEECLRRAKEIDKSIASKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGE
                     KAVRDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
                     SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGRLPHTGSVGGDSYGLIGNL
                     ISFGPMARSVSDLRLGLSVLAGSDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVD
                     AEIQNVVKSVALALQDDVAIVREVRPDCVSKAFDLHWELFLGGDRGAGFKTMLSELGV
                     NNLSWELREFLRQAEQTQFSVTQLHQRMREIDLFRLEFALFMQDYDVLISPVFPTVAK
                     PHGIGIKEISDFSYAMTHNLSGFPTISLRCGTSAEGLPINVLIAANRWKDTTSLAVAE
                     RVEQLMGGYKPPSLIHSLQ"
     gene            complement(8830..9957)
                     /gene="hemW"
                     /locus_tag="AC06IO_001868"
     CDS             complement(8830..9957)
                     /gene="hemW"
                     /locus_tag="AC06IO_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIO9875046.1"
                     /translation="MLPLSLYIHIPWCIRKCPYCDFNSHKSPDVLPEHQYIQALIEDL
                     KTDISSYNTREINSIFIGGGTPSLFSAEAYNNLFNELKRILPFAKNIEITMEANPGTV
                     EQHRFTDYRQSGINRLSLGIQSFNPNHLKILGRIHDEKQAHSAIDTARKAGFDNLNLD
                     IMHSLPNQSITQGLEDLKTALSYQPEHLSWYQLTIEPNTVFYKHTPPLPSEEEDYLLE
                     EQGFALLHNSGYQRYEISAFSKPEKQARHNINYWLFGDYLGIGAGAHGKMTTPNAIIR
                     TRKHRQPKDYLDNEKPYLAARETITGKELLFEFMLNTTRLEQSIPLELFTTATGLELN
                     QLLPLLKLANKKQLINLTDTHWQVTPLGRQYTNDLQALFLP"
     gene            10039..10599
                     /gene="nudE"
                     /locus_tag="AC06IO_001869"
     CDS             10039..10599
                     /gene="nudE"
                     /locus_tag="AC06IO_001869"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HIO9875047.1"
                     /translation="MRNKPKCKQRTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGH
                     GAVLIVALTPGESLLLVREYAAGTDSYELSFPKGVIDRNETPIEAANRELREETGFAA
                     RELHWIRSMSLAPGYFGARIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKP
                     DFTEARSIAALFLVKEWLNKRKSYDK"
     gene            10589..11581
                     /locus_tag="AC06IO_001870"
     CDS             10589..11581
                     /locus_tag="AC06IO_001870"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIO9875048.1"
                     /translation="MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLEL
                     EPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDL
                     LQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
                     ELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKE
                     TFVSTVQQRGAAVIKARGSSSAASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGV
                     DEGLIFSFPCRREHGELKVVENMEFNDFGRERFNITLNELRSERDTVKSLGLLD"
     gene            complement(11680..12600)
                     /gene="mavF"
                     /locus_tag="AC06IO_001871"
     CDS             complement(11680..12600)
                     /gene="mavF"
                     /locus_tag="AC06IO_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavF"
                     /protein_id="HIO9875049.1"
                     /translation="MQNIWLLCFYKLLLKKGLWRNIGMEDPFSVLLKSLQSVFHLEAM
                     RNGKYTFPAVQHLKEATENYNPKARNTFIELLRAIREALPYIEKWRINFNVIRKSMDA
                     LAKLHHMPTIDWNQVLSHPKVSPRFQFSALNHSHHEYDLMTWLEKKTGKPFTQLDHTE
                     LTQTLVENRDRLGFSQKLSNHLKKDPDFLYTIILRSERNFIKISQTRLILYLTDEQLA
                     RAIIKHIPVLMHKRKEPFEQIEQLIHTLNEILSNGRSVSTLLRNTDAKTILENSPLFQ
                     MYLSEEYKNRHQHPNKDPDLKTNELLKPGL"
     gene            12731..13270
                     /locus_tag="AC06IO_001872"
     CDS             12731..13270
                     /locus_tag="AC06IO_001872"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIO9875050.1"
                     /translation="MITVGNKFPEFQLKATVANDVSNAFQMISNETYQGKWLVVFFWP
                     KDFTFVCPTEIAEFGRLNSEFADRDAQILGGSTDSEFVHLAWRNQHPDLNNLPFPMLA
                     DVKRELTANLGILDDEEGVAQRATFIVDPQGITRFVMVTDLNVGRNPAEVLRVLDALQ
                     TDELCPCNWKKGEETIHIK"
     gene            13293..13808
                     /locus_tag="AC06IO_001873"
     CDS             13293..13808
                     /locus_tag="AC06IO_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIO9875051.1"
                     /translation="MLENVKESIPEVAKDILLNLTKVLDLEQSDGLSEHQILGSALAV
                     SYSLGNEVLIKGLKELVHNEQIVRAAQLAASLMAMTNIYYRFVHLTEIKEIEHIPAGL
                     RMQGMMTPGVDRVTFEILSLAVSALNGCGACVSSHARQLKEHGLSDTSLARVGRVTAV
                     IHAVHVSGRIK"
     gene            complement(13852..>14322)
                     /gene="sodC"
                     /locus_tag="AC06IO_001874"
     CDS             complement(13852..>14322)
                     /gene="sodC"
                     /locus_tag="AC06IO_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodC"
                     /protein_id="HIO9875052.1"
                     /translation="ILIGTILFSSMAIADDLTAPIYTTGPKPVAIGKVTFTQTPYGVL
                     ITPDLTNLPEGPHGFHLHKNADCGNHGMHAEGHYDPQNTNSHQGPYGNGHLGDLPVLY
                     VTSNGKAMIPTLAPRLKLSDMHNLAVMIHANGDTYSDNPPQGGGGDRIACGVIK"
BASE COUNT         3970 a         2721 c         3081 g         4550 t
ORIGIN      
        1 caataaatta tgtctacatt gcgtctacat gactttttgc gattttctga aaactactgt
       61 aactacttga ttttattggt gggcccacta ggacttgaac ctaggaccaa cggattatga
      121 gtccgctgct ctgaccaact gagctatggg cccggagagg gggtcatttt aaacttaatt
      181 tctttaatgt tctactattt ttttattttt ttaatgactg tttgtaaaaa cagtcattaa
      241 attaaccttc gtctccttca aggaagcttc ttagcttttc tgaacgtgaa gggtggcgca
      301 atttgcgtag tgcttttgct tcgatttggc gaatacgctc gcgggttacg tcaaattgtt
      361 taccaacttc ttccagggta tggtctgtat tcatttcgat accaaaacgc atacgcagaa
      421 ccttggcttc tcgtggggtc agggtttcca gtatttctaa agtggcttct cttaaacctt
      481 ccgctgtagc catatcgatt ggcgattcaa tgttgttgtc ttcaataaaa tcacccagat
      541 gagagtcgtc atcatcaccg acaggagttt ccatggaaat aggctctttg gctatcttta
      601 gaactttgcg aattttatct tcacttagct ccattttttc ggccaattct tccggtgtcg
      661 cttccctgcc agtttcttgc aaaatttggc gagaaatacg attgagctta ttgattgttt
      721 caatcatatg aaccggaata cgaatagtcc gcgcctgatc ggctatggag cgtgtaatcg
      781 cttgtctaat ccaccaagtc gcataggtag aaaacttata gccacgacgg tattcgaatt
      841 tatcgactgc tttcatcaag ccaatgttgc cttcctggat caaatcaaga aactgcaaac
      901 cgcggttggt gtattttttg gcgatagaaa tcacgagacg caagttagcc tcgaccattt
      961 cttttttagc gcgtcttgct ttggcttcgc caatggacat cttgcgatta atgtctttga
     1021 tttcactaat ggttaaacca tattcgattt caaaagaggc caacttggat tgtaagcgca
     1081 gtatttcttc ccgattttct tctatacgct gaagatcaag ttttttggca tgtttgctga
     1141 taatggtttc taaccagcct agatctgttt cctgacctgg gaaggtatct ataaacaatt
     1201 ttctggggat gcgtgctttt tcgatgcaaa gacgcataat gcttctctca aattcacgga
     1261 tatgatttcg caattggcga aaatgacgag ttaatttatc aacttgcctg gaagtcaatt
     1321 tgagtttcag aaaagattcc gacatggaat caagcaactc gaccgttttt ttattcgtac
     1381 ggccatgttc tttgagggct ttcattgcct tttcaaaggc ttcacgcagt tcatcaaagt
     1441 aaatcttcgc ttgctcagga ttggggccat catccacatc agatatgcca ccttcgccgt
     1501 cttcatcttc ctcatccaca tcaagaagta tttcttcctg ttgctcttca tccagcatgg
     1561 aaccaatgtt agtggcaggt gctatttctt catcaacatc cgcaaaacca ctaatgattt
     1621 cattaaggcg aatttcttcg gcaaggaaac gatcatagtc ttcaagaacc agttgcactg
     1681 tttccggata atgcgccagg gattttagaa cctggtaaat tccttcttca atgcgtttgg
     1741 caatgcggat ttcaccttca cgagtcagca gttctactgt gcccatttcg cgcatataca
     1801 tacgaaccgg gtcagttgtt ctacctgttt ctttatccac agaggcgagt accatggctg
     1861 cttcttcaat atctgggact tcctcagtat ttaataagag cgcaagttca tcttcactgg
     1921 gtggaagctc aaaaactttg atgttcatgc cttccagcat gctaatgatt acatcaaaat
     1981 gctccgtatc cacaatatta ggcaataagt cattaatttg actgtaagtc aaataacctt
     2041 gctctttacc caggctaatg actttcgtaa tttgtgagct ttgctgatct tggtcattca
     2101 tggtcatagg cacttctgtt ggaacaatgg gaaattacaa aaagttttaa attataaact
     2161 gggtagctgc aacataccag tatttcagta tttttatttt tcaatttcag cttgcccatg
     2221 tctttccttt aataaatttt gtaaactgag ccgttcagct tctgttaagc cagtttttct
     2281 ggatttttct atgtattgtt gtatcaatat ctcacgattt tgtttttgta aaaataataa
     2341 gacatcaata aattctttag tcagctcctg ctcaggcact tgatgatccc aggctgccaa
     2401 tttaactatt aattcaaaat aactgctatt gcgccagaat tcaatgaggg tggcagtatt
     2461 ggcattgggt ttatctttta attgttgcaa taatttcaat aaaatatgat gctcctgttc
     2521 atggagcaaa gcaggattga tatgttgtat tgctatagaa taaatttcgg ggttttgtaa
     2581 gaggagcgcg atagcaatac gtagaggcga gcgagaaata gcagcttgta cttccggctt
     2641 gctttccgat ttatcagtaa tcaaattagt tagccgatgg ctttcaatat gggttaagcg
     2701 tgacaggtca tcaataagca actgtttgta tgagccctca accatttttt gcaaataggg
     2761 tttcactgcg ttgataagct gtgtttttcc tgcagggttt gacaaattaa tgtctttact
     2821 taaagtatca aagaaaaagc gatggagagg tgttgcttgt tgcaagagct cgaggaaacc
     2881 gttttttcct tcatttctta ccaggctatc cgggtcatgt tcattcggca agaaaataaa
     2941 tccggcatct aatccggcat tcaaatgcgg taaactgctc tccaatgctc gccaggctgc
     3001 ttgtttacct gccgaatcac catcaaagca aaaaatgaga tgtttggtat gttttgccaa
     3061 tagttgtata tggaatgtgc tggtggcagt tcccaatgtt gccaccacat taaaaatgcc
     3121 atgttgtgcc aaggcaatga catccatgta accttcaaca acaataatac tgtcgggatt
     3181 tttctgctga gacaggactt gatgtaagcc atataactca cgactttttt gaaaaagtac
     3241 tgtttcaggc gagttaaggt atttgggttt ttgatcttta tcgagtacac gtccgccaaa
     3301 accaatcaca cgaccgtttc tgtcatggat ggggaacata attcgattac gatatctgtc
     3361 ataaatttta ccttcatcgc ttttaatgag cataccggta ctcaatagtt cacgctgatt
     3421 gtttgggaaa gctttttcca ggttatgcca accttcaggt gcatagccta aatgatatcg
     3481 tttggcaatt tccccactaa gacctcggtt gcgaaggtag tcaattgcca cttgtccatt
     3541 atattttaat tttttttggt aatattggct gacttctgac atgagattat agagattgag
     3601 agatgattta tgtttctctc ctgtaccatc ccttgggaca cttaaaccca agcgtgtcgc
     3661 aagggtttcc actgcatccg taaaaccttg attcagataa ttcattacaa aactgatggc
     3721 gttacctgaa gcgccgcaac caaaacaatg ataaaattgt ttttttgcaa cgacattgaa
     3781 tgatggtgtt ttctcattat ggaaagggca acatgcaata tggctgttgc ctcttttttt
     3841 caaaggaaca tatccgtcta ttaattccac caaatcagtg cggtttaata gatcatcaat
     3901 gaatggctgt gggattaagc cagacatatt ggaactcagc ttaatttcgc ctttatccgg
     3961 gtgctgactt gggccatgtc cgcgcgtcct tgtaattttg gcttaaggat agccatgact
     4021 ttacccatat cagacatttt ttcagcgcca gtagacttga tagcatcgct gattaactct
     4081 tcaatttcat tgtcagttaa aggttcaggc aggtattttg aaatgacatt caactcaaac
     4141 tgctcctggg caacgagatc gtcacggctt gctttttcaa attgcgcaat agattctttg
     4201 cgttgtttgg acattttatc caggataact aacatgcgct catcatccac ttcaatgcgc
     4261 tcatcgactt caatttgttt taccgcagca ctgattaagc gtaaagcatt gagcaagttt
     4321 ttgtcctttg cgcgcatagc gtctttaatg tcattgttga tttgttcttt tatagtcatt
     4381 ttaattcctt aaaaaacaaa aggtcacata caatgtgacc ttggtgtaat ataggacgag
     4441 ctgcgattta ctaccgaaac tgccacgaag gccaatttca aataaaaaca attaaaaata
     4501 acagagcttc caaaacaggg cactgtttaa gtgccttacg caaacttgct ttttagtgca
     4561 gtcaatcttg aaaaaatcag atcaggtagt gcttttttta cgtcggtgac caacacctcg
     4621 tcttgaacta acatcacgag atattttttt aagatgacgt tttactgcag cagcttgctt
     4681 gcgcttacgc tctgcggttg gtttttcata aaactcacgg cgacgtaatt cggttaaaat
     4741 accggctttt tcacaagagc gtttgaaacg tcgtagtgca tattctgggt tttcgccttc
     4801 ttttacacga acggtaggca ttaactagtc ctctggttat ttaatcttaa taggtgggca
     4861 attctagtgc tagattttcc acacgtcaag attaatctat acgtttcttg ctattttagc
     4921 aaattatcaa ggcattgttc tttaatttaa tggtatcttt tgtctgtgat ataattgaaa
     4981 aatgattaaa atctggaaaa ttgattccga ttattgaggt agttcatgct tgtattgggt
     5041 atagagtctt cctgtgatga aacaggtgtt gctgtctatg attcagaatc aggattattg
     5101 tcccacgcct tgcattcaca aattgccact caccgtgtac atggcggtgt tgttccagaa
     5161 ctggcatcaa gagatcatgt gaattacttg gttcctttgg tcgatgaggt attaactaaa
     5221 gcgcaaattc gtaaaaacca attggatgga atagcctaca ccgcaggacc tggattaatt
     5281 ggcgctttgt tagttggctc ctgctttgcc aaaagtctcg cctacgcctt aaatatcccg
     5341 gcattggcaa ttcatcattt ggaagcccat ctgttagccg caaaaatgga aaccccatcc
     5401 cttgattttc cctttattgc cctgttggtt tccggtggcc attgtcaatt gatagaagta
     5461 aataatatcg gtgagtatcg attattagga gacactctcg acgatgcagt aggagaagct
     5521 tttgacaaga cagccaaact gatggggatt ccctatcctg gaggagctgt gttggcaaat
     5581 ttggctgatc aatgtttatc aactccctat caatttcccc gtcccatgac tgacaggcca
     5641 ggcttggact ttagttttag cggactaaaa actcatgcct taaatacctg gaatcaaagt
     5701 gcaaaaaagg aaggcgaccg ctccgaaatt gcaaaagcat ttcaacaagc agtagttgaa
     5761 acactaatta taaaatgcaa aagagcaatt aaagaatctc aatcaaagcg cctagtggta
     5821 gccggtggag taggagccaa taaagcgttg cgtagcgctt tgcaaaaatg gattaaggat
     5881 atcaaagggg aagtatactt ccccgctctg gaatattgca cagataatgg tgcaatggtc
     5941 gcttatgcag gatgtttgcg catgatgcgg ggggagagtg atggggggtt gggagttatg
     6001 gtcaaacctc gttggcccct cgcttgaacg cccgtcttcg gcgcatcttg ccagatcatt
     6061 gctctaaaaa atgctcgtgt actcatctgt acactccgct tttttagtcg caacgatctg
     6121 tcaacctgcg ctcgaatatg ggcgtccaag ggtaggtgtc ttcggcgcat ctttgcttgt
     6181 acattaagta ggtgctgcgc cttattcctt ttctttgact gttttcgttt taggttttgt
     6241 tgatttcggt tttttagctg tttcttcctt gctggttgtc ttttttgctt ttgttgttgc
     6301 tttttttgct tttggttttt ttacagcctc agcttgttct gtcttttctg cttcatcaat
     6361 attcgtatca atgacttctt tgccaggaaa ctcttgctct gcagaagtag ctggagaagc
     6421 ctccattatt tcctctatca cactatgttt gaacttgatt ttaggctctt tcccatctat
     6481 caaacgatta aaattatctc tgtgtttgta aagaaccaga atggtgatca taaataatgg
     6541 ggggaaaata ttaagattac ccaccaatat caaggagtaa aaaggggcta aagagataga
     6601 agctattgat gctaaagagg aatagcgcca gaaatttgct accagtaacc aggttgcggc
     6661 aaccatgaca ccaattacaa aatgaaatgc taacaaggct cctattgttg tagcaacacc
     6721 ttttccccct ctgaaatgaa agaaaacagg gtacatatga ccaacaactg cagctaaagc
     6781 agtaaaggca acggttacag gttcggcact taacaatttg gccaggatga caggaagtat
     6841 accttttaaa gcatcgaata ccatgaccat aataccgtac tgcttccctc caagacggag
     6901 tacattagta gctcctgggt tttttgaacc ctcttctctt gggtcgggga gcccgaacgt
     6961 acggcacact atgatagctg aattaatgga gcccattaaa taaccaacta gtattagaaa
     7021 tataaaaagc gccaccgatt tctccttact cgggaataat ttttattatc aaccaatctc
     7081 aattaacagg caattttaag atagaaaata tgccaagact agataataga attcatttca
     7141 tgttatttca atttaattcc atgtttagtg ctggaatgag tgtaggaatt gacttgttta
     7201 tgcaaacagc aggcatggtt ggtataattt gatccaacaa gagcgttcat attgctaaaa
     7261 aagaggatgt tccatagaat ttgaattatt tgcaatagtt tgagacggat actgaatgtt
     7321 gcgttttagt cctgttcggt gtgtcactat tattctactc cattatttaa ggaaatttta
     7381 ctaaagctat ggatgccatt catctccttt ctgcaacaga aatcattcgc cgcattaaaa
     7441 tgaaagaact ctcggctgca gaggtcatgg tggcgcactt aaaccatatt gataagatta
     7501 atcctgtaat caatgcgctc accgaacgta ttccccctga ggaatgctta aggcgggcaa
     7561 aggaaattga caagagtatc gcttcgaaaa aaaatctgaa caagttaatg gggttgcctg
     7621 ttgccataaa agacgctttg tatgtgaaag gactaatttg ttcttcggca tgttcaggat
     7681 tttacaaggg agaaaaagca gtacgtgatg cgaccctggt ttctcgactg aaaaaggaag
     7741 gagcaattat tcttggctta actaatgttc ctgaattgtg tcgcggtggt gattcggata
     7801 atttaatcta tggacggacc aataatcctt atgatctggc aagaaccagt ggaggcagta
     7861 gtggtggttc cgcggctttg atcgctgcag gaggcgttcc ttttgctttg ggttccgatg
     7921 gtggaggtag tttaatgcag cctgcccatt gttgcggtat tgttgcattg aagccaacac
     7981 atggccgtct tcctcatacg ggatctgtag gtggggattc ctatggtctt attggcaacc
     8041 tgatttcttt tggaccaatg gctcgttctg tcagtgacct tcgtttagga ctctccgtgc
     8101 ttgcgggttc tgaccaatat gatccttata caaatccggt tcctgttatg cctgctgcac
     8161 cactaaaaaa attacgtgta gcctatttta cagagaatgg ttttacgcca gttgatgcag
     8221 agatacagaa cgttgttaaa tcagtagcct tggctttgca ggatgatgtg gctatagtga
     8281 gggaggtacg acctgattgt gtcagcaagg cttttgattt gcactgggag ctttttctcg
     8341 ggggcgatcg cggagcggga tttaaaacta tgttatcgga gttaggggtt aacaatctgt
     8401 catgggagtt acgagagttc ttgcgtcaag cagaacaaac tcaattctcg gtgactcaat
     8461 tgcatcaacg tatgcgtgaa atcgatttgt ttcgtctgga attcgcttta ttcatgcaag
     8521 actatgatgt attgattagt ccagtgtttc caacagtagc aaaaccgcat gggattggga
     8581 ttaaagaaat cagcgatttt tcctatgcga tgacacacaa tttaagtgga tttccaacga
     8641 tcagcctaag atgcgggact tcagccgagg ggttgcccat taatgtatta atagccgcta
     8701 atcgctggaa agatactacc tctctggctg ttgcagaacg cgttgagcaa ttaatggggg
     8761 gatataaacc cccttctcta atccactctc tgcaataaag caagaatcaa taatttttga
     8821 aacagtgaac tagggcagaa acaaggcttg caaatcatta gtatattgcc tgcctaatgg
     8881 tgttacttgc caatgagtat ctgttaaatt aatcaattgt ttcttattag ccaatttcag
     8941 taagggtaat agctgattaa gttccaatcc tgtcgctgtc gtaaataact ccaaaggtat
     9001 ggattgttcc agccgggttg tattcagcat aaattcaaac aacaattctt tacctgtaat
     9061 ggtttcgcga gcagccagat aaggtttctc attatccaga taatctttgg gttgacggtg
     9121 cttacgggtt cttattattg cgtttggggt tgtcattttt ccatgggcac cagcgcctat
     9181 acccaaataa tcaccgaata gccaataatt gatattgtgt ctggcttgct tttccggttt
     9241 ggaaaaggca gaaatttcat agcgctgata tccggaatta tgtaacaggg caaatccttg
     9301 ctcctccagc aagtaatctt cttcttcaga aggcagggga ggagtatgct tgtaaaatac
     9361 agtattcggt tctatggtta attgatacca ggataaatgc tcaggctgat aggataatgc
     9421 agtcttaaga tcctctaatc cctgcgttat actttgatta ggtaaactgt gcatgatatc
     9481 aagattgaga ttatcaaagc cggctttacg ggcagtatct atagcagaat gggcttgttt
     9541 ctcgtcatga atacggccaa gaatttttaa atgattggga ttaaagcttt gaattcccaa
     9601 agaaagacga ttgattcctg attgtctata atcagtaaat cgatgttgtt ctacagtacc
     9661 gggattggct tccattgtga tttcaatatt ttttgcaaag gggagtatgc gctttaattc
     9721 attaaatagg ttgttataag cttcggctga aaataaactg ggtgtgccgc caccaataaa
     9781 aatggagtta atttcacggg tattgtatga ggaaatatcc gttttaaggt cttcaatcaa
     9841 agcctgaata tactggtgtt cgggtaaaac atcagggctt ttgtgtgagt taaaatcaca
     9901 gtatgggcat tttctgatgc accagggaat atgaatgtac aaggataacg gaagcattac
     9961 aatttttaaa ctggcttgtt agggaatgca atagtatcat ggtagcaatt ttgctacaaa
    10021 attaagtgat gaccattgat gcgaaataaa ccaaaatgta agcaaaggac cgtgatagcc
    10081 aaatcccggt tgtttacagt tgaagaaatg tatctggaat tttccaatgg ggctcagcga
    10141 gtctatgagc gaattaaaag tcatggtcat ggcgcggtgt taatagttgc attgactccc
    10201 ggagaatcat tattattagt tcgggaatac gccgcaggta ctgattctta tgaattatca
    10261 tttcccaagg gagtgataga cagaaatgaa acccctatag aagcagctaa cagagagtta
    10321 cgtgaagaga ctgggtttgc ggcaagggag ctgcactgga ttcgttccat gtcattggct
    10381 cctggttatt ttggtgctcg cattgagctt attgttgcag agggtttgta ccattcgccg
    10441 ttgccaggcg atgagcctga gcctcctgaa acagtagagt ggcctttaca cgctgcttat
    10501 gagttattgg aaaaaccgga ttttaccgag gcgcgcagta ttgctgcgct atttttagtt
    10561 aaagagtggt tgaataagag gaaatcatat gacaaataat cgagtaagag ttgctgtcac
    10621 aggcgcagca gggcaaatag gttatgccct ggtgtttcgt attgcttccg gtcaaatgtt
    10681 tgggccaaat actgaagtgg aattgaattt acttgaactt gagcctgcac ttccctcact
    10741 ggaaggagtc gctatggagc ttgatgattg cgcatttccc ttattaaaac gcattgtttg
    10801 tactgcagat ttgaataagg caatggatgg tgttaactgg gcattattag tcggttcagt
    10861 tccgcgaaag caaggcatgg aacgttctga tttattacaa atcaatggtg gtatattcac
    10921 caaacaaggt caggcgatta atgattatgc tagcgatgat gtacgggtat ttgttgtggg
    10981 aaacccttgc aataccaatt gcctgattgc tatgaatcac gcaaaagacg tgccttctga
    11041 tcgtttttat gcaatgacca cattagatga gttaagagct cgtactcaat tggctaaaaa
    11101 agcaggcgtt gacattactg cagtgactca aatgacgatt tggggtaatc attccgccac
    11161 ccaatatccc gatttttata acgcaaaaat caatggaact tctgctgcgc aagtcataaa
    11221 tgatgaaacc tggttaaaag agacttttgt atcaaccgtc cagcaacgag gagccgcagt
    11281 gattaaggca cgaggttcgt cttctgctgc ttctgctgcc aatgcgataa ttacaggagt
    11341 taatcatttg gttactgata ctcctgctgg cgaatcgttt tcaatgtgcc gtcgctcaaa
    11401 gggagagtat ggagtcgatg aaggtttgat tttctctttc ccatgccgaa gagaacatgg
    11461 cgaattaaaa gtggttgaga acatggagtt caacgatttt ggacgcgaga gattcaatat
    11521 cactttaaac gaattacgaa gtgagcgtga cacggtcaag tctttaggct tattggatta
    11581 accagaccgg gtcaattctt gatagatttg atcctgttgt ttttgctgat taccctgctt
    11641 tgtgaatttt tcagggcagg gtgctgtcgc accttggttt tataaaccag gtttcaataa
    11701 ctcgtttgtt tttaaatcag ggtctttatt gggatgttga tgtctgtttt tatattcctc
    11761 actcaaatac atttgaaata atggcgaatt ctccaggatg gttttagcgt ctgtgtttcg
    11821 taaaagagtt gatacggaac gaccattgga taatatttcg ttgagagtat gaatcaattg
    11881 ctctatttgc tcgaagggtt cttttctttt atgcatcaaa acgggaatat gcttaattat
    11941 agctctggca agttgttcgt ctgttaagta aagaatcaag cgggtttgag aaattttgat
    12001 gaaatttctt tctgacctaa gaatgatagt gtataaaaaa tcaggatctt tttttaagtg
    12061 attgctcaat ttttggctaa atccaagcct gtcccggttc tcaaccagtg tttgggttaa
    12121 ttcagtatga tccaattgag taaagggttt tcctgtcttt ttttcaagcc aggtcattaa
    12181 atcatactca tgatgagaat gattcagtgc gctgaattga aatcgtggcg atacttttgg
    12241 atgagataag acctgattcc agtcaatagt tggcatgtgg tgcaatttag ccagagcatc
    12301 catggatttt ctgattacat taaaattaat ccgccatttt tcaatataag gcaaggcttc
    12361 tctaattgct ctaagcaatt caataaaagt atttctggct tttggattgt agttctcggt
    12421 ggcttcttta agatgttgaa ctgctggaaa agtgtacttg ccatttctca ttgcctctaa
    12481 atgaaatact gattgtaatg attttagcaa tacagaaaaa gggtcttcca taccaatatt
    12541 cctccacagg cccttcttta aaagtagttt ataaaaacat aaaagccaaa tattttgcaa
    12601 aatggattaa taatttttat tttgatttgt tttatttatt gttttgataa aaacaattga
    12661 ttttatttat ttagtgattt cacctaaatt taacaatgaa aatggtgtta atttaatgag
    12721 gagaaaactt atgattacgg taggcaacaa atttccagaa tttcaactta aagcaacagt
    12781 ggccaatgat gtaagtaatg catttcaaat gatctctaat gaaacgtatc agggtaaatg
    12841 gttagtcgta tttttttggc ctaaagattt tacctttgtt tgccctactg aaattgcaga
    12901 gtttggaagg ctaaattccg agtttgccga tcgagatgct caaattctgg gtggaagcac
    12961 agacagtgaa tttgtacact tggcctggag aaatcagcat ccggacttaa acaatttgcc
    13021 atttcccatg ctggctgatg ttaaacgaga attgacagca aatttgggca tattggatga
    13081 cgaggaggga gttgcccaac gtgcgacatt tattgtagac cctcagggaa ttactcgttt
    13141 tgtgatggtt acggatttaa atgtagggcg caatccagct gaagtattac gtgttctgga
    13201 tgctttacag accgatgaac tttgtccatg taattggaaa aaaggtgaag aaacaattca
    13261 tatcaaataa attgaaaaaa gaggcatcaa tcatgttaga gaatgttaaa gaaagtatac
    13321 cggaagtggc taaggacata ctcttgaatt tgactaaggt acttgatctg gaacaaagtg
    13381 atggattaag tgagcatcag attttaggtt ctgcattggc tgtgagttat tctttaggca
    13441 atgaggttct aattaaagga cttaaagaat tggtgcataa cgaacagatt gtgcgcgcag
    13501 cacaacttgc ggcaagcctt atggcgatga ctaatattta ctatcgcttt gttcatttaa
    13561 ctgaaattaa ggaaattgag cacattcctg caggattacg aatgcaagga atgatgactc
    13621 caggggtaga cagggtaacc tttgaaatat tgtctcttgc tgtttctgct ctcaatggtt
    13681 gtggggcttg tgtcagctct cacgcacgtc aattgaaaga gcacggttta tccgatacaa
    13741 gccttgcacg agttggacgt gttactgctg tgattcatgc ggttcatgtg agtggtcgaa
    13801 tcaaataaat cattgcattt gaagcagatt atctgcttca aatgcaaaaa actatttaat
    13861 gacgccgcaa gctatacggt cccccccgcc accttgtgga ggattgtcac tgtaagtgtc
    13921 tccattggca tggatcatga cagctaaatt gtgcatatca cttaatttca aacgaggggc
    13981 taaagtagga atcattgctt tcccattact tgtgacatat aaaacaggta aatctcccaa
    14041 atgcccgtta ccataagggc cttggtggct attggtattt tgaggatcat aatgtccttc
    14101 tgcatgcatt ccgtggttac cacaatctgc atttttatgt aaatggaaac catgcggacc
    14161 ttcaggaagg ttagttaaat ctggtgtaat caaaacacca taaggggttt gtgtgaaggt
    14221 taccttgcct attgcaactg gtttgggacc agtcgtgtaa attggtgcag taagatcatc
    14281 ggcaatagcc atgcttgaga ataaaatagt accaattaat at
//
LOCUS       DBNCWK010000072        13932 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.072, whole genome shotgun sequence.
ACCESSION   DBNCWK010000072 DBNCWK010000000
VERSION     DBNCWK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.072"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            88..447
                     /locus_tag="AC06IO_001875"
     CDS             88..447
                     /locus_tag="AC06IO_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875053.1"
                     /translation="MSNKAKNTHLKDGDNQTKQTYSNSGSPLFFSQKQITPSSGTLET
                     KKTQEGKQPTQEISLETTTDILLAAILQELKTQNMIELMKLKAQQEQEQEELETIEKM
                     KEKDDARFNEIRQTMYA"
     gene            complement(574..1644)
                     /locus_tag="AC06IO_001876"
     CDS             complement(574..1644)
                     /locus_tag="AC06IO_001876"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9875054.1"
                     /translation="MKTGRFDQARADAFAERLLSVLNDAALTLMISIGHRTGLFDVMD
                     ELDFASSEVIAEAAGCNERYVREWLNALVVGGIVDYDAHAKAYQLPAEHASFLTRKSS
                     PQNMAVVAQFIPVLAYVENGVVDAFKHGGGVPYEAYHRFHEVMAEESGQTILSSLTDK
                     ILPMVHGLVDKLNQGIQVLDIGCGYGRALILMAKTYPQSQFTGYDLCDETIAHAVHSA
                     KENKIQNIHFEKMDLSVWDEREKYDLITSFDVIHDQAKPAQVLSNVFKALKPQGIFLM
                     QDIKTSSDVARNVHHPLGALIYTISCMHCMTVSLSQNGLGLGAAWGEELAEKMLVEAG
                     FGKIEKHYLEHDIMNTYYVMRK"
     gene            complement(1708..1884)
                     /locus_tag="AC06IO_001877"
     CDS             complement(1708..1884)
                     /locus_tag="AC06IO_001877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875055.1"
                     /translation="MDKNYIRSSQYPRPVWAEVMRKHRFEALARNIKFHTRLRDGINA
                     SSARTIQDSDRFYP"
     gene            complement(2018..3217)
                     /locus_tag="AC06IO_001878"
     CDS             complement(2018..3217)
                     /locus_tag="AC06IO_001878"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIO9875056.1"
                     /translation="MIKSFRFTIFLFLASCVIFARDLQAFPAPIESNQDKLAYFQLEA
                     ETFYSPKPVTIQGNPYLTYEVFLTNVGDYPFSLENVEVLNGSKPHKVLFSYNQEELQK
                     MIYSFSGHSKHLTSDLTLRPGERGLLFFMVKFNNPGEIPKKLTHRFHLYSSKTQLSEE
                     EVRYSFLAAPAHVKTTQPLVIANPLDGKHWMAINGPSNHSIHRRARVAAHGIIYYPER
                     YAIDFMQFGSDGHIYKESPYKNENYYSYGANVYSVADGEVVAVYDSVPDNTPAGRDYP
                     VTLSNMAGNHVIIKIGKHQYALYAHLIPHSIKVTKGELVRKNQLIAKLGNSGNSDAPH
                     LHFHVIDKPSPLIGQGIPYAFEHFSTEEYRFAGDEWESPIVYEGRLKEHHHELVHENA
                     LMNFEEK"
     gene            complement(3272..3844)
                     /locus_tag="AC06IO_001879"
     CDS             complement(3272..3844)
                     /locus_tag="AC06IO_001879"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9875057.1"
                     /translation="MVLINISKSQVMSDFKFQPLGINDLNQLHQWFQEPIINHWYARD
                     KNWAIKEIKEKYEPRIVGTEHVPSFIVYKDDLPFGFVQFYILTDSLPDGIEGYESLLF
                     QQYNPRDLAGIDLFIAESQGRGKGLGVELINCFINKFLTRFKSIVIDPDVNNQQAIRC
                     YEKAGFQRTDFSQDPNHLIMIKPLSLTTGN"
     gene            complement(4035..5393)
                     /locus_tag="AC06IO_001880"
     CDS             complement(4035..5393)
                     /locus_tag="AC06IO_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HIO9875058.1"
                     /translation="MKKWIISLLLLIIIVLCSYPIIGMMALSTVKKNVDNIPKNPFLT
                     WQLKDNHRGWFCSTAVLNLLVDLPAQENKDAQGNVKVQPALHLILDIPMVIHHGPFIM
                     TNNGLRFGLAWVTTRPETHYGALINYLNQTIVSYSLPSINMQGTNNGDQGNFVFVWQG
                     LSANLKVNPKVDKASGHVTMYGMSTKVQETDVRIGKVKVSFALNRYMDDLWLGDSSIL
                     LSSVSTITNSQPVFDLQDLSMDAWAGINNNLLDFNLQLSLKKFLMNNNSYGPGQLKIE
                     LKNLDPKAMAKLNQLNYDYPSNQIAILAEIFNLVSRGASLGLNQELDTPQGHLSSSLN
                     LALTTNSFSSPEDLWQNLKGNGQFKAPIKLVKQLLLESIKSKTATPPADVSQSQATQP
                     DSTQPVDADADALKQTEELLQKLVSKGVLKVEGDYYQASFTLENTKFTVNGQPFDPAL
                     LN"
     gene            5687..6751
                     /locus_tag="AC06IO_001881"
     CDS             5687..6751
                     /locus_tag="AC06IO_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875059.1"
                     /translation="MNYKLILHPGSNLVQEFTSIPHGVTSLDLSLNNLYNISTVELIQ
                     AFANIPASVTSLNLSGNSLGFKNSDELVQILAAIPANVTSLNLSGNSLSYKSSDELVK
                     TLAAIPFTITALDLGWNDFSSKSSSEFKQALSNLPANITSLNLRGNDLGTKSSDELVQ
                     ILAAIPANVNSLNLRGNNLASKNCAELAKFLASIPASVTSLDLSANLLGLKSYTELAY
                     IFSSIPNHVVSLNLCLNCLHGLSLENLELLKDSLKPLQTVYLDYDIVKNMSKEQRQAL
                     GAVFPNIQKIILVDYYGKELHPSQSITIANLIRELSGKTDVPSLLNQSILFAKRHQTN
                     IKALNIPDELKESIQTCKPL"
     gene            6979..8493
                     /locus_tag="AC06IO_001882"
     CDS             6979..8493
                     /locus_tag="AC06IO_001882"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIO9875060.1"
                     /translation="MSYMKIEELLLKDTMRRAQIPGVSIAYVNHQGVISTQEIGLTDG
                     CGLVKMSNDPTQCPFQELVLGSKNELGVGTKNTVIAFNNHLYYVDQAAKMVQKIVLTE
                     ANKIAYKMLHAKCTETYQLADSNEHEFIATLTGRMPPTEVKPETVFGAASLSKPVFAY
                     LVQELIQTNAKNQSEAGFDQFILPEKLKHFDLDTPLFHIIPLEEFNIDGMKFDLSDES
                     IVAHSKILTARMVLSHTTGLAHGEMKFQFLPNSEEKEHGYSNVGIIYLQQVIEKLTGS
                     DLETLAQKHVFEPCGMIHSTYGLKACAANSLLTTACDYANFVKHLIHDSTIENPFVPH
                     AYMTKDKGQAGAIGIAKGNIPDAILQHVAWGLGWGLQTNNEGRVITAYHSGDMNDYRA
                     WVVIDLQDKDKKNAVVFFANSHNGHILAEQIIPKTMQMEQAANYFFSKWGFARNLAEL
                     GGKTTRLGIKSSSDSSDSSPDTSIQDEPVEIKKETEKSDISSIETYTSGRTFNT"
     gene            complement(8470..9390)
                     /gene="lelA"
                     /locus_tag="AC06IO_001883"
     CDS             complement(8470..9390)
                     /gene="lelA"
                     /locus_tag="AC06IO_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator LelA"
                     /protein_id="HIO9875061.1"
                     /translation="MAIPFKRINLNLILHLDALLSERSVSAAAEKVFVSQPAMSSSLK
                     QLRELFKDPLLIPGKGEYYLSPKAKELQPQLEQVMFFIRNILQPNERFDPFQTKRAFK
                     IALPDYVELILLPSLMAALEKLPHIVIETTIQSMADDPLLFIDEDVDLAIGAINENSL
                     SAQLKVEKLYEEEIICFASKNNPLINRSLSLEDYISNKHIAFSFNQERYSHSAEYLYK
                     RKLVRNDMLFVKNILPAIFAVANSDAVISTAPSFLINMFARQFNLGMQPLPFDLEPTP
                     IYLVTHSKSQYDEGVAWLAKLVSSSIKSPS"
     gene            complement(9574..11088)
                     /locus_tag="AC06IO_001884"
     CDS             complement(9574..11088)
                     /locus_tag="AC06IO_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIO9875062.1"
                     /translation="MFGELFASSIVLGVRYYSNQAYQAVTGLLGQIERFGIGYTREFI
                     RDPKSMIQSLTSKARSSEAGYSVVSLGYLGGNYAVITPKSQSQLTELLNQFDQEKSGR
                     RGHASFKFFSGNDFFIISDTGHAALSSRKNLSRFLVPSRLVNPTITEVEKRVDCDADI
                     QLRHTICGIVRAVMVENMLGIKQLPDNTYDVMASYRNDVKRWGAFPFPELLNFMPSLR
                     QKRDVYRAFSKGILEQEFEKLVEVLHADPNPGNTNLIAASVVSLFKDENPTLSNEELL
                     SALRSLSIEDIRKYFENPIVQSLPMILKAADNLTDAIVLCLEQIVSDPDKFAMLRDEM
                     DRSHLAIEEGIDVRALKTLPVLDAFYKESVRFDAPVAVPRYTQNGYSSDSMTIPPNTM
                     MIFDLHALAKGEQYWTNPEEFDPTRFLQQEKEQVTEEGGMNSNNKNHTVGQFPFIPFS
                     VGLRNCPAFAVTESLFKVTIAKFVSDYQLQLVKKDEEDSTLHITSRDKSFGLMQ"
     gene            11385..12299
                     /locus_tag="AC06IO_001885"
     CDS             11385..12299
                     /locus_tag="AC06IO_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875063.1"
                     /translation="MATTLIQKPSYTKNLTLNLDDYPGGVAIWGALPALFDTSNQGFD
                     RGVHVHARLADSSKKAIDATYDHVTVISGYRIFTITEEAAVHFSMSAIFDIKITSLTC
                     QHCSQLITSVGYAAVRPSRQHQCNHCGEITTTTSDCISNPIMLLKELIGDKQVKRPAV
                     IPNRTIVIDPDKYSGGIQIWGSNPSIIWTAKRLEESAIHIHAYNENGKRIIDNTYGSV
                     SLDGHKLDIEMIRVLQIQLALPNLALLLTTVYCPHCGAEQFDRGIWAVSAHNHRVCLL
                     CKQTFISQDVISNPAFDVLTHVSGAISQ"
     gene            12296..12697
                     /locus_tag="AC06IO_001886"
     CDS             12296..12697
                     /locus_tag="AC06IO_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIO9875064.1"
                     /translation="MSTLKTIFKIGALIFNEKNQLLAVHKKGKPPMELIVPGGVMEDN
                     ESDEETLRREIKEELDSDIISFQFYKQFEDKAIYEEKWLVMRTYIVTLGNAPKPANEI
                     DQLVWLGHDYHQSGYRFGSILGKQIIPDFFK"
     gene            complement(12781..>13932)
                     /locus_tag="AC06IO_001887"
     CDS             complement(12781..>13932)
                     /locus_tag="AC06IO_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875065.1"
                     /translation="KVLFDKTAKNNSGSGITYSSKNNEICTSSICMKSKLPSEVVTEH
                     LHGLQSTMVDRRNPDLPDSKPLMKAAKAHAILKTGTEIDMLTATGISEDDLPSAKGAI
                     TLTSPEELTLKATWKNKNQERGVSGRDASVFGGLGVLIISPDMKVMNIPKMNALAVPA
                     SFLAKYGAELIPLEQPFTLDAVDKSSLENLYLVEYQWDADYIQDYVMNVRGGGGLFVE
                     THPFPHVFTPLSPECRGALILGFERKDGAFDFVAFEIPFGYTMKIASNVIHGDSFFVG
                     PYAIALTETELADSVLLKQDTIERDIQRVAQIPTKAFAVYLFEEVRLANQINRKMMAD
                     KAHHEGFSGKRLGFFKVSSPKAPTEVQHSSDADQDIHDQQFGLVPCLTK"
BASE COUNT         4327 a         2879 c         2607 g         4119 t
ORIGIN      
        1 aaatttataa atattgaaaa aaacatttgc atttaactca agatttgcat taatctgaat
       61 gggactcaca aggaaaagga gatcaccatg agtaacaaag cgaaaaacac tcacctcaaa
      121 gatggagata atcaaaccaa gcaaacttat tccaattcgg gatcaccact tttttttagt
      181 caaaagcaaa taaccccctc atcgggaaca ctggaaacaa aaaaaacaca ggaaggaaaa
      241 caacccacgc aagaaatcag cctggaaaca actacagaca ttttattagc cgctattttg
      301 caagaattaa aaacgcaaaa tatgattgaa ctcatgaaac tcaaagctca acaggaacag
      361 gagcaagaag agttagaaac cattgaaaaa atgaaagaga aagatgatgc ccggtttaat
      421 gaaatccggc aaacgatgta tgcctgatta acaggacatc cttacttaac caattaacgg
      481 ataaccgtta attggtttgt cctctgataa gagtcaatgt tacctaaaag aaaagcacta
      541 acctcattag atttattaca taatctattt attttattta cgcatcacat agtaagtatt
      601 catgatgtca tgttccaggt aatgcttttc aatttttccg aaaccagctt caaccagcat
      661 tttttctgca agctcctctc cccaggccgc gccaagacct aagccatttt gtgagaggga
      721 tacagtcata caatgcatac aggagatagt gtaaataagc gcccccagcg gatgatgaac
      781 attccttgcc acatcactgg aggttttgat atcctgcatc aggaaaatac cttgaggctt
      841 caatgccttg aatacgttgc tcagtacctg cgctggctta gcctggtcat gaatcacgtc
      901 aaaactggta ataagatcat atttctcccg ctcatcccag accgacaaat ccattttttc
      961 aaaatggata ttttgtattt tattttcctt ggctgaatgt acagcatgag caatcgtttc
     1021 atcacacaga tcataaccgg tgaactggct ttgaggatag gttttagcca ttaatatcaa
     1081 ggcacgccca taaccacaac caatatcgag tacctgaatt ccctggttca gtttgtcaac
     1141 caaaccgtga accataggta aaattttgtc agtcaaggaa gataaaatcg tttgtccact
     1201 ctcttccgcc atgacttcat gaaaacgatg ataagcttca taaggaacac cgccgccgtg
     1261 tttaaaagca tccactacgc cattttccac ataggccaaa actggaataa actgtgctac
     1321 cactgccata ttctgtggcg aagatttcct cgtaagaaaa gatgcatgtt cagcgggtaa
     1381 ttgataggct tttgcatgcg catcataatc tacaatacct cccaccacaa gagcattgag
     1441 ccattccctg acataacgtt cattacaacc tgccgcctcg gctatcactt cactgcttgc
     1501 aaaatcaagc tcgtccatga catcaaacaa gccagtacga tgcccaatgg atatcatcaa
     1561 agttaatgca gcatcattga gaacagacaa aagacgctca gcaaacgcat cagctcgggc
     1621 ttgatcaaaa cgtcctgttt tcatcttgca ctcccccaaa aggtaatctt cagaatccca
     1681 tagagaaaat aatgtatcta acgttagtta cggataaaat cggtcgctat cttgtatcgt
     1741 tcgggctgag gaggcattta tgccgtctcg aagccttgta tggaatttaa tgtttcttgc
     1801 caaggcttcg aaacgatgct tacgcatcac ctcagcccaa acgggccttg gatattgact
     1861 gcttcttata taattcttat ccacaactca cgttacctca tattatagac tatgtgacat
     1921 tccatgttat gagcatcaat tttccagggc cccatttaat acttcatgta taaaagcgtc
     1981 ttcctaaaat actctatttt tatcgtctaa tattcgatta tttttcttca aaattcatca
     2041 aagcattttc atgaaccagt tcatgatgat gttcttttaa tctcccctca tagacaatag
     2101 gggattccca ctcatcgccg gcaaatcgat attcttctgt agaaaaatgt tcaaatgcat
     2161 agggtatccc ttggccaatt aaaggagatg gtttatcaat aacatgaaaa tgcaaatgcg
     2221 gtgcgtcaga attgcccgaa ttacctaatt ttgcgattaa ttgattctta cgaaccaact
     2281 cccctttagt gaccttaata ctatggggaa ttaagtgggc atagagagca tattggtgtt
     2341 ttcctatttt aataatcacg tgattgccag ccatattact cagcgtaaca ggataatcac
     2401 gtccagccgg agtattatca ggaacactgt cgtaaactgc gactacctca ccatccgcaa
     2461 ccgaataaac attggcccca taactgtaat aattttcatt tttataaggg ctttctttat
     2521 aaatatgacc atcagatccg aactgcataa aatcaatagc atatcgctct gggtaataaa
     2581 taatgccatg ggctgcaact cttgcgcgtc ggtgtatgga atgattggat ggaccgttaa
     2641 ttgccatcca atgcttgcca tccaaaggat tagcaatcac caggggctgg gtagttttta
     2701 catgagcagg ggcggccagg aaagaatacc tgacttcttc ttcggatagt tgggttttcg
     2761 atgagtaaag atgaaaacga tgcgttaatt ttttaggtat ttcaccagga ttattgaatt
     2821 tcaccataaa aaataacaaa ccacgctcgc caggtcttaa agtcaggtct gatgttaaat
     2881 gcttggaatg accactaaaa gagtaaatca tcttttgcag ttcttcttga ttataggaaa
     2941 aaagaacttt atgaggttta ctgccattta atacttcaac gttttccaaa gaaaagggat
     3001 agtcacccac attggtaaga aaaacctcat acgtgagata aggatttccc tgaatagtta
     3061 cgggttttgg agaataaaat gtttcagctt ccagctgaaa ataagccagt ttatcctgat
     3121 tggactctat tggagcggga aaggcttgca agtcccgtgc gaaaatcaca caacttgcaa
     3181 gaaataaaaa aattgtgaaa cgaaatgatt ttatcattta aaatccttgg caaatttcta
     3241 taacaacgtc ttattttcaa taaactcata attaattccc tgtggtcaat gacaagggtt
     3301 ttatcatgat gagatgattt ggatcctggc tgaaatctgt tctctgaaag cctgcttttt
     3361 cataacaacg aatggcttgt tgattattaa catctgggtc aataacaatg gatttgaaac
     3421 gagtcagaaa tttattaata aaacagttaa tgagttctac tcctaaccct ttgcctcgcc
     3481 cctggctttc ggcgataaac aaatcgattc cagctaaatc cctgggatta tattgttgaa
     3541 aaagtagact ctcataaccc tcaattccat caggtaaaga atctgtgagt atgtaaaact
     3601 ggacgaaccc aaaaggcagg tcgtctttat atacaatgaa acttggaaca tgctccgtac
     3661 ctacaattct aggttcatat ttttctttga tttccttgat tgcccagttt ttatctctcg
     3721 cataccagtg attaataatt ggctcctgga accattgatg gagctgattt aaatcattga
     3781 tgcccagagg ttgaaactta aaatcactca tgacctggct ctttgaaatg tttattaata
     3841 ccatgaggaa ttacatccaa tgtatgcccg attagcttcc tgttttcagg tcttccaatt
     3901 atcatgattc actatgcggt ggataaaaaa ttgagtgctt attctaccac agctttcatc
     3961 gccaggattt tcttgtgatt gagatagatg atcatcgcat cagcgcaggt tgagcaaatt
     4021 gcccaaccgc taatctaatt caataacgct ggatcaaatg gctgcccatt cactgtaaat
     4081 ttggtgtttt ccagagtaaa tgaggcctga tagtaatcgc cttcaacctt taaaacgcct
     4141 ttactcacca atttttgcaa gagctcttcc gtttgcttaa gagcgtcggc atcagcatca
     4201 actggctggg tagaatcagg ctgtgtagcc tgagattgag acacatcagc tggcggtgta
     4261 gctgtttttg atttgattga ttccaataat agctgcttga ccagtttgat aggcgcttta
     4321 aactgaccat tacctttcag attttgccag agatcttcag ggctgctgaa tgaattcgtg
     4381 gttaaagcta aattcagcga actggacaaa tgcccctgtg gtgtatccag ctcctgattc
     4441 agaccgagtg acgcacctct ggaaaccaga ttaaaaattt cagccaggat cgcgatttga
     4501 ttggaaggat agtcatagtt caattgattg agcttggcca tcgctttagg atccagattt
     4561 ttaagctcga tttttaattg gccaggacca tagctgttat tgttcattag aaattttttc
     4621 agggataatt gcaaattaaa atccagtaaa ttgttgttga ttccagccca tgcatccata
     4681 ctcagatctt gcaaatcgaa cactggctgt gaattagtga tggtagaaac agaagacagc
     4741 aagatagatg aatcaccaag ccataaatcg tccatgtagc gattcaatgc aaaactgact
     4801 ttgactttac caatcctgac atcggtttct tgcactttcg tactcatacc gtacatagtc
     4861 acatgaccag aggccttgtc aaccttggga ttgactttga ggtttgcact taagccttgc
     4921 cagacaaaaa caaagttgcc ttgatcacca ttgttcgtgc cttgcatatt aattgagggc
     4981 aaactatagc tgacaatggt ttgatttaaa tagttgatca aagccccata atgggtttcc
     5041 ggccttgttg tgacccaggc cagaccaaaa cgcaagccat tattggtcat gatgaaaggg
     5101 ccatggtgta tgaccattgg gatatccaga atcaaatgaa gcgctggctg cactttaaca
     5161 ttaccctgtg cgtctttatt ttcctgagca ggcaaatcaa caagcaaatt caaaacagca
     5221 gtggagcaga accatccacg gtgattatct tttaattgcc aagtcaggaa gggattcttg
     5281 ggaatattat cgacgttttt tttcaccgtg cttaaggcca tcatcccaat gatggggtag
     5341 gagcataaga cgatgattat taacagcaac aaactgataa tccatttttt catggttgtc
     5401 caaaattaat tcttggtaat taatatttag accagaaaaa gctgcttgta aaatagacct
     5461 atctatcaga aataaccttt aatacttgag cgggccagca gctcaagtct tccttcgtac
     5521 tctttcaatc tttaactgga ttttcaatag aacagatcta ctttaactcc tttcctataa
     5581 gaaatttata tacgcaatat acaaacaatt gattttaact taactctctt ggttgatatt
     5641 atgatcattt tgatcaaaca aagaactcta tttcgagatt ttcataatga attacaagct
     5701 aatattacac ccaggcagca acctggtaca agagtttacc tctatcccgc atggggtcac
     5761 ttcacttgat ttaagtttga acaacctata caatataagc actgttgaat taatacaagc
     5821 ctttgctaat atacccgcca gcgtcacttc actgaatttg agtggaaatt ccttaggctt
     5881 taaaaacagt gatgaattag ttcagattct cgcagccatc cctgccaacg tcacttcatt
     5941 gaatctgagt ggaaattcct taagctataa aagcagtgat gaactagtaa aaaccctggc
     6001 agccatccct ttcaccatca ccgcactgga tttaggctgg aatgatttca gcagtaaaag
     6061 cagctcagaa tttaagcaag ccctttccaa tttacctgcc aacatcactt ctctgaattt
     6121 gagaggaaat gacttaggca ctaaaagcag cgatgaattg gtacaaatcc tggcagctat
     6181 ccctgccaac gtcaattcct tgaatttgag aggtaataac ttagccagca aaaactgtgc
     6241 tgaattggca aaattcttgg cttccattcc tgctagtgtg acctcactgg atttaagcgc
     6301 gaatctctta ggcctgaaaa gctatactga attagcatat atcttttctt ccatcccaaa
     6361 tcacgtggtt tcacttaatt tgtgccttaa ttgtttgcat ggactatccc ttgaaaattt
     6421 ggaactcctt aaggattcgc tcaagcctct acagactgtt tatttagact atgatatcgt
     6481 caagaacatg tccaaagaac agcgccaagc tttaggtgct gtattcccta atatacaaaa
     6541 gatcatcttg gtcgattatt atggcaaaga acttcacccg tcacagtcca taacaatcgc
     6601 taacctcatt agagaacttt ctggaaagac tgatgtgcca tccttgttaa atcaaagcat
     6661 tctttttgcc aaaaggcatc aaacaaacat taaggccttg aatattcctg atgagttaaa
     6721 ggagagcatt caaacctgca aacctctctg attaatgatc ttgaagcttt cttctattgc
     6781 ttttgtgaat aagttaataa aacgatgaag caagagttaa ttaatcgtct gcattatgct
     6841 ttatctagta gcaatgcagc aaaaaccagg gcattaacgg gtttttacga tatttttgca
     6901 aaaaaaaatt ttgagtaaat cgaacacgtt aataccaaaa gtataattta tggccaaatt
     6961 atttttttga gtgaatttat gtcgtacatg aaaatagaag agcttcttct aaaagatacc
     7021 atgagaagag cccagatccc tggggtatcc attgcttatg taaatcatca aggagttatt
     7081 tccacacaag aaataggact tacagatgga tgcggccttg tcaaaatgtc aaatgatccc
     7141 actcagtgcc catttcagga gttggttctt ggatctaaaa atgaactagg cgtgggaact
     7201 aaaaacacag tcattgcatt taacaatcac ctttattatg ttgaccaggc agcaaaaatg
     7261 gttcagaaaa ttgtgttaac agaggcaaat aaaatagcct ataaaatgtt gcatgcaaaa
     7321 tgcaccgaaa cctatcaatt agctgatagc aatgaacatg aattcattgc aactctaaca
     7381 ggtcgcatgc caccaaccga agtgaagcct gaaaccgttt ttggcgcagc gtccctaagc
     7441 aaaccggtat ttgcttattt agtacaagaa ttgatacaaa ccaatgcaaa aaatcaatct
     7501 gaagcaggtt ttgatcaatt tattttacct gaaaaattaa agcattttga tttggacacc
     7561 ccacttttcc atattatccc cctggaagaa ttcaatattg acggaatgaa gtttgacctg
     7621 tctgacgaat ctatcgttgc tcattccaaa atactaaccg ccaggatggt gttatcacat
     7681 acgactggac tggctcatgg ggaaatgaaa tttcaattcc tgcccaattc tgaagaaaag
     7741 gaacatggtt attcgaacgt tggaattatt tatttacaac aagtaattga aaaactgact
     7801 ggctcagatc ttgaaacact ggcccaaaaa catgtttttg aaccatgcgg tatgattcat
     7861 agcacttatg ggctcaaggc ctgcgcagcc aattctctgc tcactacagc atgtgattat
     7921 gccaatttcg tcaagcattt aatccatgac agcacgattg agaatccctt tgttccacac
     7981 gcttacatga caaaagacaa gggacaggca ggagcaatcg gaatcgccaa gggcaatata
     8041 cccgatgcta ttctgcaaca tgttgcatgg ggcttaggct ggggactaca aactaataat
     8101 gaaggaagag ttatcacagc ctatcattca ggcgatatga atgattacag agcgtgggta
     8161 gtgatcgatt tacaggataa ggataagaaa aatgccgtgg tatttttcgc taactcgcat
     8221 aatggacata ttctcgctga acaaattatt ccgaagacca tgcaaatgga acaggctgcc
     8281 aattattttt tttcaaaatg gggttttgcc agaaaccttg ctgaattagg cggaaaaact
     8341 actcgactgg gtattaaatc atcctcagat agctctgata gctctcctga tacttccatt
     8401 caggatgaac ctgttgagat aaagaaggag acagaaaaat cagatatctc atcaatcgag
     8461 acctatactt caggaaggac ttttaatact tgaactgacc agtttggcta accaggcgac
     8521 gccctcatca tactggctct tactatgggt cacaaggtaa atgggtgttg gctccagatc
     8581 gaagggtaaa ggttgcattc ccagattaaa ctgtctggca aacatattta tcaaaaaact
     8641 tggagctgtg gaaatcaccg catcagaatt ggctactgca aaaattgcgg gcaagatatt
     8701 ttttacaaaa agcatgtcat tacgtaccag ctttctttta tataaatatt cagcggagtg
     8761 gctataacgc tcctgattaa aactaaatgc gatatgttta ttgcttatgt aatcttccaa
     8821 agataatgac ctgtttatga gtggattgtt tttgcttgca aaacaaataa tttcctcctc
     8881 atacagtttt tcaactttga gttgcgcgga tagagaattt tcattaatag ctcctatggc
     8941 caggtcgaca tcctcatcga taaacagaag tggatcatca gccattgact gaatcgttgt
     9001 ctcaataaca atatgaggca gtttttcaag cgccgccatc agggatggca acaaaattaa
     9061 ttcaacgtaa tcaggtaaag cgattttaaa tgctcttttg gtctgaaagg gatcaaatcg
     9121 ctcattcggt tgcagaatat ttcttatgaa aaacatcact tgttctaatt gtggctgtaa
     9181 ctctttggct tttggagaga gataatattc ccctttacca ggaattaata gcgggtcttt
     9241 aaatagctct ctcagttgtt ttaaagaaga actcattgcc ggttggctga caaacacttt
     9301 ctctgcagca gcactgacgc ttctctcgct tagtaaagca tccagatgta gtatgagatt
     9361 taaattaata cgtttaaagg gaatcgccat tcgatcctct cggcaattgt tctcttctta
     9421 tattagtata agaaatttaa aaatatccaa tatggataat tcattgtcaa gacagggtgt
     9481 attttgagag gggttacaat gccacttacc gaaccaagta acgctgtcaa atagacctca
     9541 ttacctggta tcaatctatt gttctttcca gtgttattgc attagtccaa aggacttatc
     9601 tctggaagta atgtgtaatg ttgaatcctc ctcatctttt ttaaccaatt gaagttggta
     9661 gtctgaaaca aacttggcaa tcgttacttt aaataaggat tcggtcactg caaatgccgg
     9721 gcaattgcgc agtcctactg aaaagggaat aaaggggaac tgacctacgg tatgattttt
     9781 attgttactg ttcatacccc cttcttcagt aacttgttct ttctcctgct gtaagaaacg
     9841 ggtagggtca aactcttcag gattagtcca gtattgttca cctttagcta aagcatgcaa
     9901 atcaaatatc atcatggtat tgggtggtat ggtcattgaa tcggatgaat agccattttg
     9961 agtatatctt ggcactgcta cgggtgcatc aaatcgcact gattccttat aaaaggcatc
    10021 taatacaggt aaagttttaa gagcccttac atctatccct tcttctatgg ccaaatgcga
    10081 tctgtccatt tcatcacgaa gcatcgcaaa tttatccgga tctgatacga tttgctccag
    10141 acaaagaaca attgcgtcag tgagattgtc tgctgctttt aatatcattg gaagtgattg
    10201 cactatagga ttttcaaaat attttctaat gtcttcaatg gataaagatc ttaaggcaga
    10261 gagcaattct tcattggaaa gagtcgggtt ttcatcttta aataaactaa ccacagaagc
    10321 tgcaattaaa ttagtgtttc ctggattagg atcagcgtga agaacttcaa ccaatttttc
    10381 aaactcttgc tctagaatac cttttgaaaa agctcgataa acatcccgtt tttgtctcaa
    10441 actcggcata aaattgagta actctggaaa aggaaatgcc ccccatcgct tgacatcatt
    10501 tctatacgat gccatcacat cgtaagtatt gtctggtaat tgcttgatgc ccagcatatt
    10561 ttccaccatc actgcgcgaa caattccgca gattgtatgt cgaagttgga tatctgcatc
    10621 acaatcgaca cgtttttcta cttctgtaat agtgggatta accagccttg agggcacaag
    10681 aaaacgggac aagttcttcc gcgatgacag agcagcatgc ccagtatctg aaataataaa
    10741 aaaatcatta ccagaaaaaa atttaaacga agcatgccct cttcgaccag atttctcctg
    10801 gtcaaactga ttcaataatt cagtcagttg agattgagac tttggtgtaa tgacggcgta
    10861 gtttcccccc aaatatccca aactgacaac actatatcct gcttcactgc ttcgtgcttt
    10921 agaagttaat gactggatca tcgatttagg atcacgaata aattctctgg tatagccaat
    10981 accaaatcgt tctatttgtc ccaaaagacc tgtcaccgcc tgataagcct gattggaata
    11041 ataacgaaca cccaacacga ttgaacttgc aaataattca ccaaacataa atcatcccaa
    11101 acaataggcc aaaaaattgg actatagtaa tacatttaga aaatattgaa cacatccagg
    11161 cgttttttta tttcatgtgg gttgattata taatgctcat tattcacccc tttcaaatac
    11221 caggaaatga ttccgacact gatacttgtc aagaattagc aagagcgata caatgacttt
    11281 gcctaaaaag ttatggtctt cagcaagtct cgattcctcc ggtgctgggt ataaagacat
    11341 aattttggtc gagggatact ttcgttttta atacgaggta tcctatggcg acgacattaa
    11401 ttcaaaaacc ctcttatact aaaaatctaa ctctcaacct tgatgactac ccgggagggg
    11461 ttgcaatttg gggtgcatta cccgctcttt ttgatacttc aaaccaggga tttgatcgag
    11521 gcgtccatgt tcacgcgcgt cttgccgatt catctaaaaa agcgatagac gcaacctatg
    11581 accatgtcac agtgatttcc ggatatcgta tttttacaat tacagaggaa gctgctgtgc
    11641 atttcagcat gtccgccatt tttgatatca aaattacatc attaacatgt cagcactgct
    11701 ctcagttgat tacttccgtt ggctacgctg cagtcaggcc atcacgccag catcaatgta
    11761 atcattgtgg tgaaatcacg actacaactt cagactgcat cagcaatcca attatgttac
    11821 ttaaagaatt gataggagat aagcaagtta aaaggcctgc agttatcccc aatcgtacca
    11881 ttgtaattga tcctgataaa tacagtggtg gcattcaaat ttggggctct aatccatcaa
    11941 ttatttggac tgctaaaaga ttggaagaaa gcgctatcca tattcatgct tacaatgaaa
    12001 atggcaaaag gattatagat aatacttatg gaagtgtttc tttagatggt cacaaactgg
    12061 atattgaaat gattagagtt ctgcaaatcc aattggcatt gccaaatttg gcattactcc
    12121 tgacaaccgt gtattgtcct cattgtggtg cggaacagtt tgatcggggg atttgggctg
    12181 tttctgcgca taatcacagg gtttgcttac tatgtaaaca aacttttata tcccaagacg
    12241 tgatcagtaa ccctgctttt gacgtactaa cccatgtttc tggagcgatc tctcaatgag
    12301 cacattaaag actatattta aaataggcgc gcttattttt aatgaaaaaa atcagttact
    12361 tgctgtacat aaaaaaggaa aaccacctat ggaactcatt gttccaggtg gcgttatgga
    12421 ggacaatgaa agcgatgaag agaccctgcg tcgagaaatt aaagaagaac ttgactccga
    12481 tatcatatca tttcagtttt ataagcaatt tgaagacaag gcaatctatg aggaaaaatg
    12541 gctggtaatg cgtacttaca ttgtcactct cggcaatgcc cctaaacctg ctaatgagat
    12601 tgatcaactg gtctggcttg ggcatgatta tcatcaaagt ggctatcgtt ttggatccat
    12661 attaggcaaa cagattattc ctgatttctt taaataaacc attcccagac attcacagca
    12721 acttttggtt cgaaccagga tataaagtcc gaacctttat gggatgagga taaagtgatg
    12781 ctatttggtt aaacaaggca ctaatccgaa ttgttgatca tgaatgtcct gatcagcatc
    12841 tgaagaatgc tgaacctcag taggtgcctt tggagaagat actttaaaga accccaacct
    12901 ttttccagaa aaaccttcat gatgggcttt atcagccatc attttacgat ttatctgatt
    12961 ggctaatcta acttcctcaa ataaataaac cgcaaaagct ttggtaggaa tttgagccac
    13021 tctttgtatg tcacgctcaa tggtatcttg cttcaaaaga acagagtcag ccaattccgt
    13081 ttcagttaat gcaatcgcat aaggccccac aaagaaagag tcgccatgga ttacattact
    13141 ggcaattttc atggtatagc caaatggtat ttcaaaagcg acgaaatcaa atgccccatc
    13201 tttgcgctca aatcccagaa tcaatgcgcc gcggcactca ggagataagg gtgtaaaaac
    13261 atgaggaaat gggtgtgttt ctacaaacaa tccaccaccg cctcttacat tcattacgta
    13321 gtcttgaata taatcagcat cccattgata ctcaaccagg tacaggtttt ccagcgacga
    13381 tttatcaaca gcatccaggg taaatggttg ctccagaggg atcagttcgg caccatattt
    13441 agctaaaaat gatgcaggta cagccaatgc attcatttta gggatattca ttactttcat
    13501 atcaggcgag ataatcagta cccccaagcc accaaaaaca ctggcatccc tcccactaac
    13561 ccctctttcc tggtttttat ttttccaggt tgcctttaga gttaattctt ccggagaagt
    13621 taaggtaata gcgccttttg cagaaggtaa atcatcctca cttattccag tggcagttaa
    13681 catatctatt tctgtgcccg ttttaagaat ggcatgagcc tttgctgctt tcattagtgg
    13741 tttactatca ggcagatcag gatttcgtcg gtcaaccatc gtggattgca agccatgtaa
    13801 atgctctgtt accacctcag atggtaattt gctcttcatg catatggaag aggtgcatat
    13861 ttcattattt ttgctggaat atgttattcc agatcccgaa ttgtttttgg cagttttatc
    13921 gaaaagtact tt
//
LOCUS       DBNCWK010000073        13887 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.073, whole genome shotgun sequence.
ACCESSION   DBNCWK010000073 DBNCWK010000000
VERSION     DBNCWK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13887
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.073"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..2324
                     /gene="lepA"
                     /locus_tag="AC06IO_001888"
     CDS             <1..2324
                     /gene="lepA"
                     /locus_tag="AC06IO_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LepA"
                     /protein_id="HIO9875066.1"
                     /translation="NEIPIESIPNFVLPSGLSYDSSPADFYLVLLILMPKVSEKKEQF
                     SQALEKLQEMPALGKQIQQLRSGYDLPITMDVQLPSLQEAEESKDSLNTEDPLKEELK
                     SQIKLCQSYLETAKQIDSLMKDHGQTTNEAKALIEQLRLISNQPLKNEDINSIKEDLS
                     TLANQIQSLVSELNQLPLPNLSGEKIDPPLEKQEIAVDKAIPHTVESMHPAQVIQTGN
                     DSPIEKQQLDNEEETPVVLKSLRPLPVIHKESESITQKLQVDNGQETPVVLDSTCTPQ
                     AIHKELESTTEKLQVDNGHEIPVVLESTRSPQIIHTESESVGKKQHVEVEQEIPVALE
                     LIRPSSMVEKDSVATVEKQQVTERQETPIVLESTRLSPMLQKDSGLSGEKQHKEIEQE
                     ISVVPELTLRTQVVQQSSESSLEQQVPNGQETLAVVKSSPSEPLRPSTPEVPAISRKP
                     NGLSLFNGHDELSEDNILAFFDEAGNQISISSEEDSETFTIDRGKVISSGIDKAFKEK
                     PVSTGHESDKLSNPQHKESAPSISPLPPSSILLKQKLDSFHVQNMEYIKQHSEEIQLW
                     YKGLYDAAQSSCVNEALGLKALHLLKDILFELKNQNDLSVLLAYKRMCPNPLQDIQNI
                     LRLKPALPILDESIDEEQQLKNWPEELQKFHQQYVKLKKEHPLEAELFIQAIHSLISI
                     KHLMELPDAKTSNREAMPLITQDPRYEPLKRHRGFIRAWEYIEDFFRMLIGKLTGQDE
                     YEYSKRPCFFKTRSHRLLEEVDTMLHPMAPTSS"
     gene            complement(2393..3760)
                     /gene="glmM"
                     /locus_tag="AC06IO_001889"
     CDS             complement(2393..3760)
                     /gene="glmM"
                     /locus_tag="AC06IO_001889"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIO9875067.1"
                     /translation="MSQRKYFGTDGIRGHVGLSNINPEFVLKLGWAVGCVLANGARKK
                     VVIGKDTRVSGYMLESALEAGLSAAGVDVALLGPMPTPGIAYLTQTLRANAGIVISAS
                     HNLFEDNGIKFFSADGGKLPDSVELAIEAQLEKQLQTVPSAKLGKATRINDAAGRYIE
                     FCKSTIPSLSRLSNLKIVVDCANGATYHIAPNVFSELGADVVPIGIKPDGFNINQECG
                     STAPELLREKVIAVGADIGIGLDGDGDRVILVDSLGNLVDGDQIIYIIAKDRHQRGVL
                     HGGVVGTLMSNYGLELAITSLGVPFQRSKVGDRYVLETLRVKDWKIGGETSGHIVCLD
                     KTTTGDGIVAALQVLSIMVKQNKALHELTAGIQLLPQTLVNLKTNNAALLASNPDVIQ
                     AVKNLEKHLNGEGRVLLRPSGTEPLLRVMVEGANASIVKQQAQMLCDDISQIDKKMTE
                     NLPST"
     gene            complement(3824..4699)
                     /gene="folP"
                     /locus_tag="AC06IO_001890"
     CDS             complement(3824..4699)
                     /gene="folP"
                     /locus_tag="AC06IO_001890"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIO9875068.1"
                     /translation="MNSEHFLGWLERKSQPPSSLVFEKPLIMGVLNVTSDSFYDGGKY
                     LSVDRACDQALKLIACGADLIDIGGESTKPGAPPVPLDVELSRVLPVIKQLRALADVC
                     ISIDTNKPEVMEAAIDAGANVINDVYALRRDGALNVAAQLDVPVCLMHMKGEPRTMQE
                     NPSYPEGIMKELQHFFEERIDKCQKAGLNKNKLILDPGFGFGKCVKDNLKLIYHLNEL
                     RSFGLPILLGVSRKSTIGAVLNNEVEQRLVGSITLGIYAALKGTAIIRTHDVDETNQA
                     LTMINAIYQAAKEIS"
     gene            complement(4732..6642)
                     /gene="ftsH"
                     /locus_tag="AC06IO_001891"
     CDS             complement(4732..6642)
                     /gene="ftsH"
                     /locus_tag="AC06IO_001891"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIO9875069.1"
                     /translation="MVKNLFLWLIIAIVLVSVFSNFGPRNSVAEKISYSQFLQEVDQG
                     MINSVTIEDNKIIKGVTKNNKRFVTYMPMQDNALLGELLKSKVDVSGQEKQQESFLLH
                     LFINWFPMLLLIGVWIFFMRQMQGGGGRGAMSFGRSRARLLGEDQVKVTFADVAGVDE
                     AKEEVKELVDFLRDPTKFQNLGGRIPRGVLLVGSPGTGKTLLARAVAGEAKVPFFTIS
                     GSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFEGNEGVIVVAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILKV
                     HLQKVPVDSHVEVKAIARGTPGFSGADLANLVNEAALFAARANKRKVGMIELDKAKDK
                     IMMGAERRSMVMDDNEKKLTAYHEAGHAIVGLSVPEHDPVYKVSIIPRGRALGVTMFL
                     PEQDRYSHSKRRLESQLCSLFGGRIAEELIFGPESVTTGASNDIMRSTEIARKMVTTW
                     GLSALGPLTFGEEEEEIFLGRSVNKHKEMSDRTAQQIDDEVRAIIDRNYQRAKEILET
                     NIDKLHLMAQSLIKYETIDTNQIQEIMSGKEPTPPEDWGSTKPMDKAESVNDAPAKPI
                     NSETIENPAEGH"
     gene            complement(6765..7385)
                     /gene="rlmE"
                     /locus_tag="AC06IO_001892"
     CDS             complement(6765..7385)
                     /gene="rlmE"
                     /locus_tag="AC06IO_001892"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HIO9875070.1"
                     /translation="MNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKES
                     LIKPGMTVVDLGASPGGWTQYASEKMKGSGRLVALDILPMDALPNVEFILGDFREDNV
                     LQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLV
                     KIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYLLAKGYNL"
     gene            7488..7754
                     /gene="yhbY"
                     /locus_tag="AC06IO_001893"
     CDS             7488..7754
                     /gene="yhbY"
                     /locus_tag="AC06IO_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIO9875071.1"
                     /translation="MDTSFKQSLKAKAHHLKPVVLLGAKGLTEAILAETNVALLAHEL
                     IKVKINGAEKEDRMQMAEELCEQLHAELVQMIGNTLVLYRKNEE"
     gene            complement(7805..9781)
                     /locus_tag="AC06IO_001894"
     CDS             complement(7805..9781)
                     /locus_tag="AC06IO_001894"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIO9875072.1"
                     /translation="MNYRPDIDGLRAIAILLVLIYHGGLSLFPSGFIGVDVFFVISGF
                     LITTIIHESLNKNDFSFFDFYNRRLWRLQPVFVALIVVTTLLSVLFYLPDDLIEFSRS
                     ARKASLFISNLFFNKTTTGYFSPDTHQLPLLHTWSLSIEWQCYLVLPLVMYGLYRFLN
                     KGQVIYVVHGLAILFFLLALHYSQTAPAQTYYQFSSRIFEFLIGSCIALSPRIEFSIN
                     KSILVILGSSALFFIIYTATLDNILLGYPNWRTLFVCLATGLLIALGTNFPSLAIVNT
                     LSFRPLVFIGVLSYSLYIWHWVVFSMLRYQNIEERPEVLFIVYGIVFILAYLSWRYIE
                     KPTKKFRHIPFRYTVVILLLIPVLFSHLSSLVIKSHSGYPQRFNQELVRVYKQLKSYE
                     SPYRALCIDNTRADNAWKCKIGAKEQGSRTGLMIGDSFSNHYWGFMDVLGKAAGVSIL
                     AQGTSSCISLPGIYLYDWWHFKDKIYQDCYEQTEKYYRMIQENHYDYVIIGQVWENYV
                     SAHIINQLGDDYSAELTKKRLADALDRALGVIISSGAKPVLIESTAVTHGNLHQCFFK
                     HIKLRQSYNSEECRFTLTSSDNETWLNQLFDNMRRKYPQLIIIDPKQVQCRDNVCHSD
                     LNGIPVYRDEGHITDYASYQLGYLYVREFENPLI"
     gene            9967..11694
                     /gene="frgA"
                     /locus_tag="AC06IO_001895"
     CDS             9967..11694
                     /gene="frgA"
                     /locus_tag="AC06IO_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore biosynthetic protein FrgA"
                     /protein_id="HIO9875073.1"
                     /translation="MALAYGNFHELSHQLRFLLFEIGIGLPQNSVDYFITLAHKNTLK
                     RLQHASIKEGLIQSAIASHHIHDFIDQLQIKLKNSMPESKFFQWRKIREALDESIANE
                     ALAYAYRQNWNTQLRNEAMHYKSLWTWINNELSPYQTLLFLEQWGSLRHPYHPAFSAK
                     TGFTRREVLQNSPEFQAKVSVHWCALNKTKIQSISPKIDYANQISQEFPKEYFYWREK
                     LLFSHINPDDYYPIPVHPWQWRNQLQMAFASLIDNKSLILLPHHQTLIPSLSPDIMMP
                     TQSTQCTLKLATTLSTSMAGKLDNSNDMVLLTRWIDSLLAKTNYYQNTLFICKNLESM
                     SAYDQTLSECNRVKLLFGLYQNPLHKIRQDQRAVPLPALLTDSPCSNTPLLIEIIKAS
                     GLHPTTYFTEYCYKMLFGQLHLLLKYGLALEVEQHNILVIFDDNKPQGIIIKEPNNLK
                     LCNHELFKNVQKPNAPDSLSIYTKDLNQVRTLFIQGTLKNHLHHLIGCLRNEYQIPSR
                     TLWGLARQVMQTVFKDLSKDIDPRILSWQQHLLLHDNWEHQPELLLSLHSKINRNITI
                     KEYNPLSEI"
     gene            11954..12514
                     /locus_tag="AC06IO_001896"
     CDS             11954..12514
                     /locus_tag="AC06IO_001896"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIO9875074.1"
                     /translation="MILKHIPNTLTLLRLVLIAPFLMYLFHHEYVKAFYIFIAAGLTD
                     GLDGWLARHFQWQSFFGSFVDPLADKLLVASSFISLALLNSLPWWLVLLVFLRDFTIS
                     MGVLAWYYFIQRKLDFEPSRLSKLNTGFQLTLVTLCLFELAYFRFPYDLVDILIYLTA
                     FTTVTSYLDYVWTWSRKAWSPKESLK"
     gene            12511..13203
                     /gene="hda"
                     /locus_tag="AC06IO_001897"
     CDS             12511..13203
                     /gene="hda"
                     /locus_tag="AC06IO_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HIO9875075.1"
                     /translation="MNKQLALAIKLNDEATLDDFNWGNNTLLQQQLIEMLSFKADRLL
                     YLWGPKGSGKSHLLQACCQAINLTQSAIYLPLAFLKEWGPQSIEGLEDQTLICIDDIN
                     TIANDSTWEEALFHLYNKIKDSERSLLIISGNQPPIKCDIKLADLRSRLSWGLVIQLN
                     ELNDDEKINTLKMRAAKRGFELPESVGHFLLNRCSRNMHDLYELLNRLDDASWEAHRK
                     ITIPFVKNILKI"
     gene            complement(13318..13707)
                     /locus_tag="AC06IO_001898"
     CDS             complement(13318..13707)
                     /locus_tag="AC06IO_001898"
                     /inference="COORDINATES: protein motif:HMM:NF024776.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9875076.1"
                     /translation="MGVLMMNRQERQRKAVLEMVRQRRISLVQASQQCELSYRQTLRI
                     YQRYEEKGDAGLIHGNRGRVSSRKNPHQASIINRYIERYEDFGPTLASEYLERDGFHV
                     DHETLRKWLIKEGLWKKQRKRSPVVTP"
BASE COUNT         4487 a         2962 c         2339 g         4099 t
ORIGIN      
        1 taaatgaaat accaatagaa agtataccaa attttgtttt accaagtggc ctttcttatg
       61 actcttcgcc ggccgatttt tatttggtac ttctcattct catgccaaaa gtttcggaaa
      121 agaaggaaca attttcacaa gcgttggaaa aacttcaaga aatgcctgct ctgggcaaac
      181 aaatacaaca actaagaagt ggatacgatt tacctataac tatggatgta caacttccaa
      241 gtttgcaaga agctgaagaa agtaaagatt cattgaatac agaggatcct cttaaagaag
      301 agttaaaaag ccagattaaa ctatgccaat cttatcttga aacagccaag caaattgatt
      361 cgcttatgaa agatcatgga caaacaacca atgaagcaaa agcacttatt gagcaattgc
      421 gtttaatttc aaatcaacca ctaaagaacg aagacataaa ctctattaaa gaagatttat
      481 ctactcttgc caatcaaatc caatccctag tgtctgaatt aaatcaatta cctttgccta
      541 atctgtccgg tgaaaaaatt gatcctccat tggaaaaaca ggaaatagca gtagataaag
      601 caatccctca tactgtggaa tcgatgcacc ctgcccaagt aattcagaca ggcaatgatt
      661 ctcccataga aaaacagcag ctagacaatg aggaagaaac gccggttgtt ctgaaatcat
      721 tacgccccct tccagtcatt cataaggaat ctgaatctat cacccaaaaa ctgcaagtag
      781 acaatggaca agaaacgccg gttgttctgg attcaacatg cactcctcaa gccattcata
      841 aggaacttga atctaccact gaaaaactgc aagtagacaa tggacacgaa ataccggttg
      901 ttctcgagtc aacacgttct cctcaaatca ttcatactga gtctgaatct gttggtaaaa
      961 agcagcatgt agaagtagag caagagatac cggtcgcact ggaattaata cgaccctcat
     1021 ctatggttga aaaggattcc gtagctactg tagaaaaaca gcaagtaact gagagacaag
     1081 aaacaccaat cgtccttgaa tcaacacgac tctctcctat gcttcagaaa gacagtgggc
     1141 tttctggcga aaagcaacat aaagagatag agcaagaaat atctgtcgtt ccggaattaa
     1201 cactccgcac ccaagtggtt cagcaaagct ctgagtccag tttagaacaa caagtaccca
     1261 acggacaaga aacactggct gttgtcaaat caagcccatc ggaaccattg agaccatcaa
     1321 cccctgaggt tccagctata tccaggaaac caaatggctt gtcattattt aatggtcacg
     1381 atgaattatc agaagataat attttagcct tttttgatga ggctggtaat caaattagca
     1441 tctcatctga agaagattct gagacgttta ccatagacag gggaaaagta atttcctcag
     1501 gaattgataa agcatttaaa gaaaagcctg tttctacagg ccatgagtca gataaattat
     1561 caaatcctca acacaaggaa tctgccccta gcatctcccc tttaccacca tcttctatcc
     1621 tgcttaagca aaaattagat tcatttcatg tacaaaacat ggaatatata aagcaacatt
     1681 cagaagaaat acagctatgg tataaaggtt tatatgatgc agcgcaaagt tcttgtgtaa
     1741 atgaggcatt ggggcttaaa gcacttcatc ttttaaagga catcttgttt gaattaaaaa
     1801 atcaaaatga tttatctgta ttactggctt acaagcgcat gtgcccaaac ccattgcaag
     1861 atatacaaaa tattctccgc ttaaaacctg ctctgcctat acttgatgaa tcgattgatg
     1921 aagaacagca gttaaagaat tggcctgaag aattgcaaaa atttcatcag caatatgtaa
     1981 aattgaaaaa ggaacatcca ttggaagctg aattatttat tcaggcaatc cattcactta
     2041 tttcaattaa acatttgatg gaacttcctg atgcaaaaac ttcaaaccga gaggccatgc
     2101 ctctcattac tcaagatccc cgatatgagc ctttaaaacg gcatagggga tttataagag
     2161 catgggaata tattgaagat tttttccgaa tgttaatagg taagttaaca gggcaagatg
     2221 aatatgagta ttcaaaaagg ccttgttttt tcaaaacaag atctcaccga ttacttgaag
     2281 aggttgacac aatgctccac cccatggctc caacaagtag ttaatacctt atactgtgaa
     2341 acagaggggc ttttggaaaa gcccctgcat tagaacttaa aaagacagat gctcaagtgg
     2401 acggtaggtt ctcagtcatt tttttatcga tctgactgat gtcatcacat aacatttgtg
     2461 cttgttgctt gactatcgat gcattagcac cttccaccat aactctcagt aaaggctcag
     2521 tgccagaagg tctgagtaac actctaccct ctccattaag atgtttttcc aaatttttaa
     2581 ccgcctgaat tacatcagga ttagaagcta acaaggcagc attattcgtt tttaaattca
     2641 ctaaagtttg aggtaacaat tgaatacctg cagttaactc atgcagtgct ttattttgct
     2701 tcaccatgat tgaaagaact tgcagtgccg ctacaattcc atcacctgtt gttgttttat
     2761 ccaaacaaac aatatgtcca gaagtttcac cgccaatctt ccaatctttc actcttagcg
     2821 tttcaagaac atacctgtca cctacttttg agcgttgaaa aggaaccccc aatgaggtaa
     2881 ttgctaattc aagaccataa ttactcatca acgtgcctac taccccccca tgcagtactc
     2941 ctcgttggtg tctgtccttg gcaatgatat aaataatttg atcgccatct acgagatttc
     3001 ccagtgaatc cactaaaata actcgatccc cgtctccatc caaaccaata ccaatatcag
     3061 ccccaacagc aatcaccttt tctcttaata attcaggcgc tgtcgaacca cattcttgat
     3121 tgatattaaa accatctggt ttaattccta taggaactac atcagcaccc aattcagaga
     3181 atacatttgg ggcgatatga taggttgccc cgttggcgca atccacaact attttcaaat
     3241 tggatagtcg agatagggaa ggaatcgtag atttacaaaa ctctatgtaa cgacccgctg
     3301 catcattaat acgtgttgct ttacccagct tggctgaagg tacagtttgc aactgttttt
     3361 ctaattgggc ttcaattgca agctctacac tatctggaag ttttcctcca tcagcagaaa
     3421 aaaatttaat gccattgtct tcaaataaat tatgagaagc gctaatcacg atgcccgcat
     3481 tagccctcaa tgtttgtgtt agataggcta ttccaggagt aggcattggt cctaataatg
     3541 ccacatcaac tccagctgcg gacaaacctg cttccaaagc cgactccaac atatagccag
     3601 acactctggt gtccttacca atcactacct ttttcctcgc tccgttcgca agaacgcagc
     3661 ctactgccca gcctagtttt aaaacaaatt cagggttaat atttgataat ccaacgtgac
     3721 cccgtattcc atccgtacca aaatatttac gttgactcat ccttacctac cttattattt
     3781 aatttttcta atctcagttg tcttctgatc atttcatgag ctattaactg atttctttag
     3841 cagcttgata aattgcatta atcatagtta atgcttgatt cgtttcatcc acatcatgtg
     3901 tcctgataat tgcagtgcct ttaagagcag catatattcc tagcgtaata cttccaacca
     3961 aacgttgctc aacttcatta ttcaatacag caccaattgt gcttttgcgt gaaacgccca
     4021 aaagaattgg caatccaaaa gaacgcaatt cgttcagatg ataaattaat tttaggttat
     4081 ctttaacgca ctttccaaaa ccaaagccag ggtcaagaat taatttattc ttattcagcc
     4141 cggctttctg gcatttatcg atgcgctctt caaaaaaatg ctgtaattcc ttcattattc
     4201 cttctgggta agaaggattt tcttgcatag ttcgaggctc tcctttcatg tgcattaaac
     4261 aaacaggaac atctaactgc gcagcaacat tcaaagcacc atctcttctt aaagcataaa
     4321 catcatttat aacattcgca ccagcatcaa ttgccgcctc catcacttca ggcttattag
     4381 tatcaataga aatacacaca tctgcgaggg cacgaagttg tttgattacg ggaagaactc
     4441 ttgatagttc aacatctaaa gggactggcg gagcaccagg tttggttgat tccccaccaa
     4501 tatcaatgag atcagctcca catgcaatta attttaaggc ctgatcacag gctctatcaa
     4561 cagacagata cttgccgcca tcataaaatg agtccgaggt aacatttaac acacccataa
     4621 ttaatggctt ttcaaaaact aaggaggaag gaggttggct ctttcgctca agccaaccga
     4681 ggaagtgttc agaattcaca gggtactctc gataaataac tactgataaa attaatgtcc
     4741 ttctgccgga ttttctattg tttcgctatt aataggcttt gcaggagcat cattaaccga
     4801 ctcagcctta tccatcggtt ttgttgaccc ccaatcctcc ggaggggtag gctctttacc
     4861 agacataatt tcttgaattt gattggtgtc tatcgtttcg tatttgatga ggctttgagc
     4921 cattaaatgt aatttatcga tattagtttc caatatttct tttgctcttt gatagttcct
     4981 atcaataata gcacgaacct cgtcatcaat ttgctgtgct gttctatcgg acatctcctt
     5041 atgtttattc actgagcgtc ctaaaaatat ttcttcctct tcctcaccaa aagttagagg
     5101 gcctaaagca gataagcccc aagttgtaac cattttgcga gcaatctcag tagaccgcat
     5161 aatgtcattg gaagcacccg ttgtcacact ttcaggacca aaaattaatt cctctgcaat
     5221 tcgaccacca aacagactgc ataattgact ttccaaacgg cgtttgctgt gactatatct
     5281 gtcttgttct ggtaaaaaca tagttacacc caaagctcgg cctcgaggaa ttattgatac
     5341 tttataaact ggatcatgct caggaactga cagaccaacg atagcatgac ctgcttcatg
     5401 atatgcagtg agttttttct cgttatcatc cattaccatg gaacgtcttt cagctcccat
     5461 cataatttta tcttttgctt tatccagttc aatcatacct actttgcgtt tattagccct
     5521 tgcagcaaat aatgccgctt cattaacaag attggctaaa tccgctcctg aaaaacctgg
     5581 ggtgccgcgt gcaatagctt tcacttctac atggctatct acagggacct tttgtaaatg
     5641 aacttttaat atttgttcac gacctctgat atcaggcaaa ggaacaacga cttgacgatc
     5701 aaagcggcct ggacgtaaaa gcgcggggtc caaaacatcg ggacggttag ttgccgctac
     5761 gacaataact ccttcattac cttcaaatcc atccatttca acaagcaatt ggttaagtgt
     5821 ttgctcgcgt tcatcatgac cgccacccaa gccggcgcct ctgtgtctac ctactgcatc
     5881 gatttcatct ataaagatga tacatggcgc atgctttttg gcttgctcaa acatatcacg
     5941 cactcgggac gcaccgaccc caacaaacat ctcaacaaaa tcagaacctg aaatggtaaa
     6001 gaaagggact ttcgcttccc cggcaactgc cctggctaat aatgttttac ctgtaccagg
     6061 ggaaccaacc agtaaaacac cgcggggaat gcgtccaccc aaattttgaa actttgttgg
     6121 atcacgtaaa aaatcgacaa gctctttcac ttcttctttg gcttcatcaa cacctgcaac
     6181 atctgcaaag gtgactttaa cctgatcttc acctaatagc cgagctcttg atctgccgaa
     6241 agacattgcg ccgcggccac cgccgccttg catttggcgc atgaaaaata tccatacacc
     6301 tatcaataac aacattggga accagttaat aaataaatgc aaaaggaaac tttcctgttg
     6361 tttttcctga ccactgacat cgactttgct tttcaacaat tcgcctaaca aagcattgtc
     6421 ttgcatcggc atgtacgtga caaagcgttt attattcttg gttacgccct tgatgatttt
     6481 gttatcctca atcgtaacag agttaatcat cccttgatcc acttcctgta aaaactggct
     6541 ataagaaatt ttctcagcaa ctgagttacg aggaccaaaa ttactgaaaa cagagacaag
     6601 aactatcgct attatcagcc ataaaaataa atttttaacc atgtcgttca atgtattaac
     6661 ctcgttagtt acttacaatt ttgttgcgag acccaaaata gctcttgcac agctcatatc
     6721 atccaaaaca acgccaagca tatttataaa tatatataaa actactataa attatagccc
     6781 tttgccagca aataggtttc ttttgaacga gatcttgaag cggatggctt acggattacc
     6841 actttttcaa aagacgctcg tgcctgtttg actaattcat caaaacccga gccatgaaaa
     6901 attttaacta gcatattacc accgggtttt aacatttttt ccgcgaaatc aaaagccagc
     6961 tctaccagat acatggctct tggaatatct attgctgagc ttccactcat atttggggcc
     7021 atatccgata ataacaagtc taaagtcctt tgaggaatca aattaattaa ttcttgcaaa
     7081 acattatctt cacgaaaatc cccaaggata aactccacat taggcaaagc atccatgggt
     7141 aaaatatcta atgcaaccag acgaccactt cctttcatct tttctgaggc gtattgggtc
     7201 cagccaccag gagaggcccc taaatcaacc actgtcatgc ctggtttaat gagtgactct
     7261 ttgtcatcta tttctttgag tttatatacg gctcgacttc gatatccttc agcctgcgct
     7321 ttttttacat aaacgtcatc aaaatgttct tgcagccaac gtttactgct tttagtacga
     7381 ttcataacga ataaagataa aacaattttt cctatgatac agaattttac taccttttcc
     7441 cagcaaaacg tgcaataatt taccatttta ttatctggga tatcacagtg gatacgtctt
     7501 ttaaacaatc acttaaagcc aaggctcatc atttaaagcc cgttgtgctc cttggtgcca
     7561 aaggcttaac tgaagctata ttggcagaaa cgaatgtagc cttgttagca catgaattaa
     7621 ttaaagtcaa aattaatggg gctgaaaagg aagatagaat gcaaatggcc gaggagcttt
     7681 gtgaacaatt gcatgccgaa ttagttcaga tgataggaaa tacactggtt ctgtatcgta
     7741 aaaatgaaga ataaaatttg taaaatcgtt ttgtagtctg gagaatcaaa gcaagccaac
     7801 ctttttaaat caaaggattt tcaaactccc gcacatataa ataacccagt tggtatgaag
     7861 cgtaatcagt aatatgccct tcatcgcgat atactggaat cccgttcaaa tcggaatggc
     7921 aaacattatc gcgacattga acttgtttag ggtcgattat tatcaattga gggtattttc
     7981 ttcgcatgtt atcaaacaat tggtttagcc aagtctcatt atctgaactg gttaaagtaa
     8041 agcggcactc ttctgagtta taactttgtc ttaatttaat atgcttaaaa aagcattgat
     8101 gtaaatttcc atgcgttacc gcggttgatt cgattaatac aggttttgct ccagaagata
     8161 ttatcacgcc aagtgctctg tccaaggcat ctgccaaacg ctttttggtt aattctgcag
     8221 aataatcatc gccaagttga ttaataatat gggctgaaac ataattttcc catacttggc
     8281 ctataatcac ataatcgtaa tgattctctt gaatcatgcg ataatatttt tccgtttgct
     8341 cataacaatc ctgatatatt ttatctttga aatgccacca atcataaaga tatatgcctg
     8401 gtaaggaaat acaagaagag gttccttgag ccaatattga aacaccggca gctttcccta
     8461 acacatccat aaacccccaa taatgattgg aaaaagaatc acctatcata aggccagtcc
     8521 tgctaccttg ctcttttgct cctattttgc atttccacgc attgtctgct ctggtattat
     8581 ctatacataa cgctctgtat gggctttcgt aactctttaa ttgtttataa accctaacca
     8641 gctcctgatt aaatcgttgt ggatagccag aatgggattt aataactaaa gagcttaaat
     8701 gagaaaataa aacagggatc agcaataaaa tcacaaccgt ataacgaaaa ggaatatgtc
     8761 tgaatttttt cgtcggtttt tctatatatc gccaggataa ataagctaaa ataaaaacaa
     8821 tgccataaac tataaataaa acctcaggcc gctcttcgat attttggtac cttaacattg
     8881 aaaagacgac ccagtgccaa atataaagag aataagataa aactcctata aacactaatg
     8941 gtcgaaaaga gagagtattt acaatagcaa gtgaaggaaa atttgtacct aatgctatta
     9001 acaaacccgt tgccagacac acaaaaagtg tacgccaatt aggatatcca agaagaatat
     9061 tatccagtgt agcggtataa attataaaaa ataatgctga tgaacccaaa ataactaaaa
     9121 tagacttatt gattgagaac tcaattcgag ggcttaaagc gatgcaagaa ccgattaaaa
     9181 actcaaaaat acgactggaa aattgataat aggtttgggc aggagctgtc tgtgagtagt
     9241 gcaaagccag caaaaaaaat aaaatggcca acccatgcac aacatagata acctgccctt
     9301 tatttagaaa ccgatataag ccatacatca ccagtggcaa aaccagataa cattgccact
     9361 caattgaaag agaccaggta tgcagcaaag gaagttgatg ggtatcgggt gaaaaatatc
     9421 cggtggttgt tttattaaaa aacaggttgg aaataaacag tgatgcttta cgcgcactac
     9481 gactaaattc aattaaatca tcaggcaaat aaaacaatac gcttagtaga gttgtcacca
     9541 caatcagagc aacgaaaaca ggctgtaacc gccataacct tcgattataa aaatcgaaga
     9601 atgaaaaatc attcttattc agtgattcat ggataatagt agtaatgaga aaacctgata
     9661 taacaaaaaa tacatcaaca ccgataaacc ctgatggaaa aagcgagagg cccccatgat
     9721 aaattaagac caataaaatt gcgatggccc ttaatccatc tatatcgggt ctataattca
     9781 tgccacttac tcatttacat aataaaagta gaaaatatta tcatttaact cagaatacag
     9841 aaacttttta tccaaaatca aactctattg ccaattaata atattttatt tgccccacct
     9901 cttgcaaatg agaatgatta tcatttatat ttaatttata acaaaataat ccttcaggag
     9961 ataataatgg ccctggcgta cggtaatttt catgaactca gccatcaatt acgcttttta
    10021 ctatttgaaa ttggcatagg actaccacaa aatagtgtgg attattttat taccttagct
    10081 cataaaaata ccctgaagcg tttacagcat gcctccatta aggaaggatt aattcaatca
    10141 gccattgcaa gtcaccatat ccatgatttc attgaccaat tgcagataaa actgaaaaat
    10201 tcaatgccgg aaagtaagtt ttttcaatgg cgaaaaatca gggaagcatt agatgaatcg
    10261 attgccaatg aggctttggc ttacgcctac aggcaaaact ggaacaccca attaagaaat
    10321 gaagccatgc actacaagag tctgtggaca tggataaata atgaactatc tccgtatcaa
    10381 acgttattat ttctggaaca atggggcagt ttgaggcatc cctatcaccc agcattcagc
    10441 gcaaaaacag ggtttacgcg aagagaagta ctccaaaact ctcccgaatt ccaggccaaa
    10501 gtcagtgtac attggtgtgc attaaataaa acaaaaattc agtcaataag cccaaaaatt
    10561 gattatgcca accaaatttc tcaagaattt cccaaagaat atttttattg gcgtgaaaaa
    10621 ttgttattta gccacatcaa ccctgatgat tattatccaa ttcctgttca cccttggcag
    10681 tggaggaatc aattacaaat ggcgtttgca tctttaattg ataataaatc cctcatcttg
    10741 ttacctcatc accaaacact aataccttct ttatcacctg atattatgat gccaacacaa
    10801 tccactcaat gtacacttaa actggctacc actttaagca cctcaatggc tggaaaactt
    10861 gataattcta atgatatggt attgcttacc agatggatcg attccctgtt agcaaaaaca
    10921 aactattacc aaaatacctt gtttatatgt aaaaacctgg agagcatgag cgcctatgat
    10981 caaactctct ctgaatgcaa tcgagtaaaa ttattgtttg gcttatatca aaacccactc
    11041 cacaaaataa gacaggatca aagagcagtt ccattacctg ccctattaac tgattcccct
    11101 tgtagcaata caccattact cattgaaatt attaaagcta gcggcctgca cccaacgact
    11161 tatttcactg aatattgtta taagatgtta tttggacaat tgcatctatt gctaaaatat
    11221 ggattagcac tagaagtgga gcaacacaat attttagtca tcttcgatga caataaacct
    11281 caggggataa ttataaaaga gccaaacaac cttaagctat gcaatcatga actgtttaaa
    11341 aacgttcaaa aacccaacgc tccagactct ttatccatct atacaaaaga tcttaatcag
    11401 gttagaaccc ttttcatcca gggaacatta aaaaatcatc tacatcactt gattggctgt
    11461 ttacgtaatg agtatcagat tccttcaaga accttatggg gattagctcg ccaagtcatg
    11521 caaactgtat ttaaagactt atccaaagac attgatccgc gtattctaag ttggcaacaa
    11581 catctattgc ttcatgataa ctgggagcat caacctgaat tgttattaag tctgcattcc
    11641 aaaatcaatc gaaatattac aataaaggaa tacaacccat tatcagagat ctaagctcta
    11701 ctggacttac gaaaaacccc atgcgctctt gttccaatac taaaatatga gaacttctcg
    11761 aggccgggat gtggatacta tgagtcaata aatcccaatt gatttctcaa taaggtgaca
    11821 gagacaatgg tattgtataa actaattcac attcgccatt tgaaacactc aaataataga
    11881 gtattgttgg cacaatatat tccctcctca gcgataaata aaaaaagagg aggaactaat
    11941 tttcgtataa cagatgatac taaaacatat tcccaatacc ttaacattgc taagattagt
    12001 tttaatagct ccttttctaa tgtatttatt tcatcatgag tacgtgaagg ccttttatat
    12061 atttatcgcc gcaggattaa ctgatggttt ggatggttgg ttagccaggc attttcaatg
    12121 gcaaagtttt tttggctcat ttgttgaccc tcttgcagat aaattgttag ttgcctccag
    12181 cttcatttct ttagctctct taaactcctt gccctggtgg ttagtgcttc ttgttttttt
    12241 aagagacttc actatttcaa tgggagtatt agcttggtat tactttatac agcgcaaact
    12301 ggattttgag ccctctcgac tcagtaaact caataccggc ttccagttaa ctttagtcac
    12361 tttatgttta tttgaattag cttactttag attcccctat gatttagttg atatactcat
    12421 ctatttaact gcctttacca ccgttacttc ctaccttgat tatgtttgga cctggagcag
    12481 aaaagcctgg tcccccaaag aatcgttaaa atgaataaac agctggccct ggcaataaaa
    12541 ttgaatgatg aagcaaccct ggacgacttc aattggggta ataatacctt gttgcagcag
    12601 caattaattg aaatgctttc ttttaaggca gacaggttgc tctatctttg gggccctaaa
    12661 ggaagcggca aatcacattt actgcaagct tgttgccaag ccattaattt aacccaatca
    12721 gctatttacc ttcctttagc ttttttaaaa gaatggggac cacaaagcat tgaaggatta
    12781 gaagatcaaa ctttaatttg tatcgatgat attaatacca tcgctaacga ttcgacctgg
    12841 gaagaagctt tattccattt atataataag attaaggact ccgaacgttc gcttttaatt
    12901 atatcaggca atcaaccccc aataaaatgt gacattaaac ttgcggattt acgctccaga
    12961 ctaagttggg gattagttat tcagttgaat gaattaaacg atgatgaaaa aataaacaca
    13021 ctcaaaatgc gcgcagcaaa acgcggattt gaacttcctg agagtgtcgg acatttttta
    13081 ttaaaccgtt gctctcgaaa catgcatgat ttatatgagc tcctcaaccg gctagatgat
    13141 gcatcctggg aggctcatcg taaaatcacc ataccttttg ttaaaaatat tttgaaaata
    13201 taagagttta ttgactctcc tttataaatt tatttaagat ccttgatgag gatccaccgt
    13261 aattctttta tcatttccca tatattgcct atccacagag ttactgagat gaccgtttca
    13321 tggagttacg acaggactgc gttttctttg ctttttccat aaaccttctt ttattaacca
    13381 tttccttaat gtctcatgat ccacatgaaa accgtctcgt tccaaatatt ctgatgctaa
    13441 tgttgggcca aaatcttcat atctttctat atatcggttt ataatcgatg cttgatgcgg
    13501 gttctttcgg cttgagactc gaccacggtt accgtggatt aggcctgcat cgcctttttc
    13561 ctcatagcgt tgatatattc ttagtgtttg ccgataactt aactcacatt gctgggacgc
    13621 ttgcaccaga cttatccgtc tttgccttac catctctaac acagcctttc tttgccgctc
    13681 ttgcctattc atcatcagta ctcccataca accagtctcc tttttagtcc tcagggaact
    13741 tttaaggtaa ctgaatgtga catttttatg gagttaatga tgtgacagtt tcatggagtt
    13801 acgacaattg ttattcttca atttgacaaa aatgaaaacg agtctatatt taaagagttg
    13861 cacaattttt tatctgctat tcgctat
//
LOCUS       DBNCWK010000074        13862 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.074, whole genome shotgun sequence.
ACCESSION   DBNCWK010000074 DBNCWK010000000
VERSION     DBNCWK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.074"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            130..1362
                     /locus_tag="AC06IO_001899"
     CDS             130..1362
                     /locus_tag="AC06IO_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875077.1"
                     /translation="MSVSRVTAWFVWVIVSIFYAYQYVLRVMPNIMLDDITQQFHIDA
                     IIFGQFSGIYYIGYSLMHLPIGIMLDRFGPRKVMTGCIMLTVIGLLPILFAENWVYPV
                     IGRALIGIGSSAAILGTFKIIRMTFKEQHFPRMLSFSVMIGLVGAVYGGGPVSYMCQL
                     MGYKMVVEIFVGIGIVLAGITYLIVPDAQPSRQDSVISNIRMVFGNSKVILLCCFAGL
                     MLGPLEGFSDVWGSGFLKMAYSLNANAANYLPSMIYIGMCFGAPVLSLIAEKTGYYLG
                     TIIASGVVMFLVFAMLVGGVLTVPSITISFILVGICCSYQILAIYKVSTYVPDNLAGL
                     TTAIANMIIMIFGYGFHSVIGYVVNMYGGTHVARAFVYGVGVIPLTLAVGVVGFLFLA
                     YQEKNFTPDASFASPELR"
     gene            1417..1731
                     /locus_tag="AC06IO_001900"
     CDS             1417..1731
                     /locus_tag="AC06IO_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HIO9875078.1"
                     /translation="MYMLYFHVPETHLDVVKNAVFAAGAGHVDNYIHCAWQTLGEGQF
                     MPLPGSQAFIGEINQLEKVPEYKVETICAGEKIKEVVAALKKAHPYESPSYQVVRIET
                     EF"
     gene            1922..3475
                     /locus_tag="AC06IO_001901"
     CDS             1922..3475
                     /locus_tag="AC06IO_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIO9875079.1"
                     /translation="MLKILVLLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSA
                     FQNANWWKVFNDPTLTALINNGYHNNLSLQIAGVRVLQARAQLAQSVGELYPQQQAMV
                     GDYNYNRIGGNQLQGLLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASLAAY
                     DSALVTLTSDIASTFIKIRTSEELIKVTKANIQVQAMSLKIAKDRYYGGQTSLLDVEQ
                     AATELAETQAKLPQYVSDLQHQKDILAVLLGTTPDKVDGLLKKNKGIPKASAVVEVGI
                     PKEVIARRPDVYQARMEAIAQSAAIGAVKANLYPSLSLVGTFVFASNNIGNSTISDIF
                     NWSNRNIVAGPSLAWSLLNYGQITNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITR
                     YVEGQKTVSSLKSANASAIESTRLALVRYKEGEANYTTVLNAEQQQLRVQTSLVNATG
                     ELALSLVGLYRSLGGGWQIRGKNDIVSNQIKSEMAARTNWGSLLKQENHLPPDSNGQR
                     MKQLYLPTW"
     gene            3481..4635
                     /locus_tag="AC06IO_001902"
     CDS             3481..4635
                     /locus_tag="AC06IO_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9875080.1"
                     /translation="MNSQNKQNLVKTVAIVFFGIVFVYLVYDHFTRAQVVTPAPPVVI
                     VQKPKLMKMGQYVTQTGNTVAFQSVDLVARIEGYLDAIKFTDGAFVKKNQELFIIEPE
                     PYLEQLRAAEAKVAAQKANYAYAKSEYARQQRMYKQNATSLNSVEKWKAKVEEAQAEV
                     DKSEADATNAAITYGYTHVSAPFDGRMGRHLVDVGNLVGHGAATDLATIEQIDPIYVY
                     FNLNELDLIRIRNAARKRGYKPMELVKIPVEVALQDEKGFPHKGTLDFVNTGLNASTG
                     TMEFRALLPNKGYPLVPGLFVQVRVPISEPSPKLTIPDTSVQYDQIGPYVLVVDKDNY
                     VLTKRVTLGGLENGVRAITKGLDAQDSVIVSGLQNATPNHQVTPKTEQPE"
     gene            4695..7847
                     /locus_tag="AC06IO_001903"
     CDS             4695..7847
                     /locus_tag="AC06IO_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIO9875081.1"
                     /translation="MISKFFIERPVLANVIALFIVFIGIIAIAVLPVSQYPAIVPPTI
                     QVTTTYPGADAKTLINTVALPIEQQVNGVEDMLYMQSTSTSGGTYTLIVTFAIGTDLN
                     YAQVLVQNRVQAAMAQLPESVQKQGVVVQQKSTAILQFITLTSKNNEYDGVFLDSYAT
                     INMQDELSRLPGVGNVVVFGSGSYAMRVWLDPKKMLAFSLNPSDVLNAISYQNKEVSA
                     GQLGAPPTVGKQAYQFTVNVPGQLSDPKEFENIIIKTIDTPPDQNSSASSSAQVVRIR
                     DVGRVELGSESYNQLANLNGKPTAAIGIFQLPGANALDVAQEVRKAVAKMAKQFPPGL
                     EYSIPFDTTVFVKASIEEVYKTLFEAGILVLIVIVVFLQNFRASLVPATTVPVTIIGT
                     FFAMLLLGYSINLLTLFALVLAIGIVVDDAIVIVEGVTQHIEKGMSPKESAILAMKEL
                     FGPIIGITLVLMAVFVPAGFMPGLTGSMYAQFALVIAATAFISAINAMTLKPTQCALW
                     LKAPDTSKPKNIFFQAFDRIYNPIESAYVKFIDRLVHRSGKVCLIGIILVACAIYGLT
                     RIPTGFIPMEDQGYLMLSIQLPDGASLGRTDEVVSRLAKKASEVGGVDNVIAIDGISL
                     LDNNSLLSNAGVIYVIFKDWSVRGKSENLRALYTKFNAIAKETLDAKVLVVVPPPIQG
                     LGMSGGFQMQVELQDGTFDYRKLQQATDQMINTGRQYPQLQNLMTTFRASVPQVAAPI
                     NRTKAESLGVRVADAFDTLQTYLGSSYVNLFTKFGQVFPVYVQADASSRVSSEDLRNY
                     YVRNQSGSMVPLGTLTDVGPAVGPSIISLYNLYPSSNINGVAARGYSSGQGIQVMEKL
                     AKEQLPPGISYEWTSTAYQEKVAGNLSYFIFALSLVLVYLILSGQYENWLIPSAIILS
                     VPLTLVGTVLALGSLGMDNNMYTQIGLLLLIALATKNAILIVEVAREQREIHNKSVLE
                     AAVIGAKTRFRPILMTSFAFIMGVMPLVFATGAGANSRRSIGIAVSSGMLASTCLAVV
                     FVPVFYVLLQTWQDKRKAKH"
     gene            complement(8214..10967)
                     /gene="sidC"
                     /locus_tag="AC06IO_001904"
     CDS             complement(8214..10967)
                     /gene="sidC"
                     /locus_tag="AC06IO_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidC"
                     /protein_id="HIO9875082.1"
                     /translation="MVINMVDVIKFKEPERCDYLYIDENNKVHILLPIVGGDEIGLDN
                     TCQTAVELITFFYGSAHSGVTKYSAEHQLSEYKRQLEEDIKAINSQKKISPHAYDDLL
                     KEKIERLQQIEKYIELIQVLKKQYDEKNDIRQLRTGGIPQLPSGVKEIIKSSENAFAV
                     RLSPYDNDKFTRFDDPLFNVKRNISKYDTPSRQAPIPIYEGLGYRLRSTLFPEDKTPT
                     PINKKSLRDKVKSTVLSHYKDEDRIDGEKKDEKLNELITNLQNELVKELVKSDPQYSK
                     LSLSKDPRGKEINYDYLVNSLMLVDNDSEIGDWIDTILDATVDSTVWVAQASSPFYDG
                     AKEISSDRDADKISIRVQYLLAEANIYCKTNKLSDANFGEFFDKEPHATEIAKRVKEG
                     FTQGADIEPIIYDYINSNHAELGLKSPLTGKQQQEITDKFTKHYNTIKESPHFDEFFV
                     ADPDKKGNIFSHQGRISCHFLDFFTRQTKGKHPLGDLASHQEALQEGTSNRLHHKNEV
                     VAQGYEKLDQFKKEVVKLLAENKPKELLDYLVATSPTGVPNYSMLSKETQNYIAYNRN
                     WPAIQKELEKATSIPESQKQDLSRLLSRDNLQHDNLSAITWSKYSSKPLLDVELNKIA
                     EGLELTAKIYNAKRGREWWFKGSRNEARKTQCEELQRVSKEINTLLQSESLTKSQVLE
                     KVLNSIETLDKIDRDISAESNWFQSTLQKEVRLFRDQLKDICQLDKYAFKSTKLDEII
                     SLEMEEQFQKIQDPAVQQIVRDLPSHCHNDEAIEFFKTLNPEEAAKVASYLSLEYREI
                     NKSTDKKTLLEQDIPRLFKEVNTQLLSKLKEEKAIDEQFHEKLSQLADKIAPEHFTRN
                     NIIKWSTNPEKLEESNLSEPIKSVQPPTTKQTSIQFREAMGEITGRNEPPTDTLYTGV
                     IKK"
     gene            complement(11118..13844)
                     /gene="sdcA"
                     /locus_tag="AC06IO_001905"
     CDS             complement(11118..13844)
                     /gene="sdcA"
                     /locus_tag="AC06IO_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdcA"
                     /protein_id="HIO9875083.1"
                     /translation="MMNMVDKIKFKEPERCEYLHIDKDNKVHILLPIVGGDEIGLDNT
                     CETTGELLAFFYGKTHGGTKYSAEHHLNEYKKNLEDDIKAIGVQRKISPNAYEDLLKE
                     KKERLEQIEKYIDLIKVLKEKFDEQREIDKLRTEGIPQLPSGVKEVIQSSENAFALRL
                     SPDRPDSFTRFDNPLFSLKRNRSQYEAGGYQRATDGLGARLRSELLPPDKDTPIVFNK
                     KSLKDKIVDSVLAQLDKDFNTKDGDRNQKFEDIKKLVLEEYKKIDSELQVDEDTYHQP
                     LNLDYLENIACTLDDNSTAKDWVYGIIGATTEADYWPKKESESGTEKVSVFYEKQKEI
                     KFESDTNTMSIKVQYLLAEINFYCKTNKLSDANFGEFFDKEPHATEVAKRVKEGLVQG
                     AEIEPIIYNYINSHYAELGLTSQLSSKQQEEITEKFTQRYHIIENSPHFDEFFVADPD
                     KKGNIFSHQGRMSCHFLDFFARQTKGKHPLGDLAGHQEALQAGTSNRLHHKNEVVAQG
                     YEKLDQFKKEVVKLLAESKPKELLDYLVATSPTGAPNYSMLSKETQNYIAYNRNWPAI
                     QTELEKTTDIPENQKQDLVRLLSRNNLQHENLSAITWSKYSSKPLLDVELNKIAEGLD
                     LTAKIYNEKRKSEWFKGSRNEARKTQCEELQRVSQEINALLQSESLTKSQVLEKVLNS
                     IEALDKIDRDISAEYNLFKSTLQKEVQSFREQLKDICQLDNYAFKSTKLDEIISLEME
                     EQFQMIKDPAVQQIVRDLPSHCHNNEVIEFFMTLNPEEAAKVASYLSLEYREINKSTD
                     KAKLLNEDIPNLFKEVNMQLLSKLKEDSVLGEGVYEKLAQLADKIPPEHFTRNNIRKW
                     SANPEKLEESNLGELLKSSDSSLSEMARKYRETINEMTGRNEPPRETVRHTI"
BASE COUNT         3817 a         2560 c         2810 g         4675 t
ORIGIN      
        1 gaatttaaaa tcaaaaatga gggttttatt ttgttttttg caatccaatt tcaagggaag
       61 taagataaaa tactttatga taattatttg atctgcgtgg tctttttatt ttcttttctg
      121 agggtgatta tgtctgtttc acgagttaca gcgtggtttg tgtgggtcat agtatccata
      181 ttttacgcat accaatatgt tctgagagtt atgcccaata ttatgttgga tgatattact
      241 cagcaatttc acattgatgc cataattttt gggcaatttt ccggtattta ttacattggt
      301 tattctttga tgcatttgcc tattgggatt atgctcgatc gttttggccc aagaaaagtg
      361 atgacaggat gcatcatgtt gaccgtgata ggtttacttc ccattttgtt tgctgaaaat
      421 tgggtttatc cagtgatagg aagagccctg attggtatag gttcatcggc tgccatttta
      481 ggaacattta aaataatacg tatgacattt aaggagcaac attttccaag gatgctgagt
      541 ttttcagtca tgataggact tgttggggcg gtatatggtg gcggtcctgt aagttacatg
      601 tgtcaactta tgggttataa aatggtggtg gaaatatttg taggcatagg aatagtgctg
      661 gccggtatta cctatctgat tgttcccgat gcacagcctt ctcgccaaga tagtgtgatt
      721 tctaatatca gaatggtttt tggtaatagt aaagtgatcc tgttgtgttg ttttgcggga
      781 ttgatgttgg gaccactgga aggtttttct gatgtatggg gctctggctt tttaaaaatg
      841 gcatacagtt tgaatgctaa cgcggcaaat tatcttccat caatgattta tattggcatg
      901 tgttttggcg cacctgtctt aagtctgatt gcagaaaaaa cagggtatta tcttggaact
      961 atcatcgcat caggcgtggt tatgtttctg gtatttgcta tgcttgtcgg aggtgttctg
     1021 actgttccca gtataacaat aagttttatc ctggttggca tttgttgctc ttatcaaatt
     1081 ttggctattt ataaagtatc cacttatgtc cccgataatc ttgctgggtt aactactgca
     1141 attgcgaata tgattattat gatttttggg tatggttttc attctgtaat tggttatgtc
     1201 gtcaatatgt atggggggac acatgtagct cgcgcttttg tttatggagt tggggtgatt
     1261 cctttgacat tggctgttgg cgtggtgggt tttttgtttc tggcctatca agagaaaaat
     1321 tttaccccgg atgcttcctt tgcctctcct gaattacgat aattactggc atttctatct
     1381 tgtgtggtaa taaatatcaa aggaggcata gccatcatgt atatgctgta ttttcatgtt
     1441 ccagaaactc atcttgacgt agtaaaaaat gcggtttttg cggcaggcgc agggcatgtt
     1501 gataattata ttcattgtgc ctggcaaact ttaggagaag gtcagtttat gcctttacca
     1561 ggtagtcagg catttattgg agaaattaat caattggaaa aagtgcccga atacaaggta
     1621 gaaacgattt gcgccggaga gaaaatcaaa gaggtggttg cagctttaaa aaaagctcat
     1681 ccctatgaaa gcccttctta tcaagtggta cgtattgaga cagagttcta aatattagag
     1741 cgagaaattc aggcaggtcg aatcaccaga gggttggtca attagtaaga atttgtttct
     1801 tcaggatact tgttcaattg agtattcata gataatatct aatgattgat gaaccaatag
     1861 ttcaaatcca tcttcaatct acataagctg cttttgaaca gatagggaaa tactggtata
     1921 catgttgaaa atccttgttc tcttaagttg tttgattcta agctcctgcc tggtcgggcc
     1981 aaattttaaa gagcctaaaa aagatgctgc caattattgg cttacttcaa gccctgcaat
     2041 aaaacaatcc gcctttcaaa atgcaaattg gtggaaagtc tttaatgatc ccactttaac
     2101 cgcattaatc aataatggtt atcataacaa tctctccttg caaatcgcag gagttcgcgt
     2161 tttgcaagca agggcacagc ttgctcaatc ggttggggag ctttatcccc agcagcaagc
     2221 tatggtagga gattataatt ataatcgaat cgggggcaat caattacaag ggcttctgcc
     2281 ccccgatttt ttaacagcca gtcttggatt ttccgcaagc tgggaacttg atttttgggg
     2341 aaaataccga agagccattc aatctaatga tgccctgttc cttgcctctc tggccgctta
     2401 tgattccgca ttggttactc tcacgtcaga tattgccagt acttttatta aaatccgcac
     2461 cagcgaagaa ttaatcaaag taacaaaagc gaatatccag gtacaggcca tgagtcttaa
     2521 aatcgcaaag gaccgttatt atggtggtca aaccagttta ctggatgtgg aacaagcagc
     2581 aacagaactt gcggaaaccc aagcaaaact gcctcaatac gtcagtgatc tccaacatca
     2641 aaaagatatt ttggctgtat tgcttggcac cactcccgat aaagtggatg gcttgctcaa
     2701 aaaaaataaa gggataccaa aagcttcggc ggtagtggaa gttggaattc ccaaagaggt
     2761 gatagcgcga aggccggatg tctatcaagc cagaatggag gctatagccc aatcagccgc
     2821 aattggcgcc gtgaaggcta atctttatcc ctcactttca ttagtcggga cttttgtttt
     2881 tgcttcaaat aatatcggca attctaccat tagtgatata tttaactggt ctaatcgtaa
     2941 tatagtcgca ggcccgtcat tggcatggtc tttattaaat tatggtcaaa tcacaaatgc
     3001 ggtacgagct caagatgctg ctttccaaca agccttgctc aattatctta atttggtgct
     3061 caaagtgcag caggaagttc aagacaatat tacgcgctat gttgaggggc aaaaaacagt
     3121 ctcttcactt aaatcagcca atgcctctgc tatagaatca acaaggcttg ctttagtgcg
     3181 atataaggaa ggagaggcta attacaccac tgtattgaat gcagaacagc aacaattgcg
     3241 agtacaaacc tcgctggtca atgccacagg tgagttggct ttgtctctgg ttggcttata
     3301 ccgctcgctt ggaggcggtt ggcaaatacg aggcaagaat gatattgttt caaatcaaat
     3361 aaaaagtgaa atggctgccc gtactaattg gggtagttta ttgaagcaag aaaatcattt
     3421 gcctccagac tctaacggac aacgaatgaa acaactatat ttaccaacct ggtgatgaga
     3481 atgaactctc aaaataaaca aaatttagtt aaaaccgtag caattgtatt ttttggtatt
     3541 gtctttgttt atttagtata tgaccatttt acaagagccc aggtagtaac cccagcacca
     3601 ccagttgtga tcgttcaaaa gcccaaatta atgaaaatgg gacagtacgt tactcagaca
     3661 ggcaatacag tagcatttca atcggtagat ttggtggcac ggattgaagg ttatttggat
     3721 gccataaaat ttactgacgg cgcttttgtc aaaaaaaatc aggaattatt tattattgaa
     3781 cccgagccct atcttgagca actgcgtgct gctgaagcca aggtagcagc tcaaaaagca
     3841 aattatgcct atgccaaatc agagtatgcc agacagcaac gcatgtataa acaaaatgcc
     3901 acgtctctta acagtgttga aaaatggaaa gcaaaagttg aagaagcaca ggcggaagtg
     3961 gataaatcgg aagctgatgc tacaaacgcc gcaattacat atggttatac ccacgtttcg
     4021 gctcctttcg atgggcggat gggaagacat ttagtcgatg tgggtaattt ggttgggcac
     4081 ggtgccgcaa cggatttggc taccatagaa caaatcgacc ctatttacgt ttattttaat
     4141 ttaaatgaac tggatttaat caggattaga aatgcagcac gcaagagagg atataaacca
     4201 atggaattgg tcaaaatccc tgttgaagtg gctttgcaag atgaaaaagg atttcctcat
     4261 aaggggacat tggattttgt aaatactggt ttaaacgctt caactggaac catggagttt
     4321 cgtgccttgt tacctaacaa gggatatccc ctggtacctg gtttatttgt gcaggtccgg
     4381 gtaccgatat ctgaaccttc tccaaaatta acaatacccg acacctctgt tcaatacgat
     4441 caaattggcc cctatgtcct tgtggttgat aaagacaatt atgtgcttac caaacgcgtg
     4501 acacttggcg ggctagaaaa tggggtaagg gctattacta aaggcctcga tgctcaagat
     4561 agtgtgattg tttccgggtt acaaaatgcg acgcccaatc atcaggttac gcctaaaact
     4621 gagcaacctg agtaagtatg gccaaaaaat tgtaagaatt taagaccctt tattcaggga
     4681 gcgaaacaac tgaaatgatt tccaaatttt ttattgaaag acccgttctg gccaatgtca
     4741 ttgccttgtt tattgtattt atagggataa tagcgattgc tgttttgcct gtgtctcaat
     4801 accctgccat tgttcctccc actatccagg ttactacgac ctatcctggc gctgatgcaa
     4861 aaactttgat taataccgtc gctttgccaa ttgagcaaca agtcaatggg gttgaggaca
     4921 tgctgtacat gcaatcgaca agtactagtg gaggcaccta tactttaatt gtcacgtttg
     4981 ctataggcac ggatctgaac tatgcgcagg ttctggtgca aaatcgagta caagcggcga
     5041 tggctcaact ccccgaatca gtacaaaagc aaggtgttgt cgttcaacaa aaatcgactg
     5101 ctattcttca atttattact ttgacttcaa aaaataatga atacgacggc gtatttcttg
     5161 acagctatgc gaccatcaat atgcaagatg aattatcccg tttacccgga gtagggaatg
     5221 tggtggtttt tggttcagga agttatgcga tgcgtgtttg gctcgatcct aaaaagatgc
     5281 ttgctttttc tttgaaccca agtgatgttt taaatgccat cagctaccaa aacaaagagg
     5341 tgtcagctgg acaactgggt gctcctccta ctgttggcaa acaagcctat caatttacag
     5401 tgaatgtgcc tggacaactc tctgatccca aagagtttga aaacattatt attaaaacga
     5461 ttgatacccc acccgatcaa aattcctctg ccagtagctc tgctcaggta gtacgtattc
     5521 gtgatgtagg gagagttgag ttgggatccg agagctataa tcagctcgct aatcttaatg
     5581 gtaaacctac agcagcaata ggtatctttc aacttcctgg tgccaatgcg ttggatgtcg
     5641 cgcaagaagt tcggaaagcc gtggcgaaaa tggcaaaaca gtttcctccg ggtcttgaat
     5701 attctattcc atttgatacc accgttttcg tcaaagcttc aattgaagaa gtatataaaa
     5761 cactttttga agcaggaatt ttggttttaa tagtgattgt tgtattttta caaaattttc
     5821 gcgcttcatt agtacccgcg acaacggttc ctgtgactat cattgggaca ttttttgcca
     5881 tgcttttatt gggctatagt attaatttgc tgactttatt tgctttggta ttagcaattg
     5941 gaattgtggt tgatgatgcc attgttattg ttgagggtgt gactcaacat attgaaaaag
     6001 gcatgtcacc taaagaatca gccatacttg caatgaaaga attatttggc ccgattattg
     6061 gtatcacttt ggtattaatg gctgtttttg taccggccgg ttttatgcct gggttaacgg
     6121 ggtcaatgta tgcacagttt gcgttggtta ttgctgcaac tgcctttata agcgcaatta
     6181 atgccatgac gttaaagccg actcaatgtg ctttatggtt aaaggctccc gatacgagta
     6241 aaccaaagaa tatattcttt caggcctttg atcgaattta taatcccatt gaatcggctt
     6301 acgtaaagtt tattgatcgg cttgtacatc gtagtggtaa agtttgtttg attggaatca
     6361 ttttagtggc ttgtgccatt tatggcctga cgcgtattcc aactggcttt attcccatgg
     6421 aagatcaagg ctatttgatg ttaagtattc agctgccgga tggcgccagt ttaggacgta
     6481 ctgatgaagt agtgagtaga ttagctaaaa aagcatctga agttgggggt gtagataatg
     6541 tgattgctat tgatggtatt tcattattgg ataacaattc tctgctttct aatgccggtg
     6601 tcatctatgt tatattcaaa gattggagtg tcagaggaaa atctgaaaat ttacgagctc
     6661 tttataccaa attcaatgca atagccaaag aaacccttga tgcaaaagtg ttggtggttg
     6721 tgccccctcc cattcaaggt ttagggatgt cagggggatt tcaaatgcaa gttgaattac
     6781 aagacggtac ttttgattac aggaaattac agcaagctac tgatcagatg atcaatacag
     6841 ggcgtcaata cccacagttg caaaacctta tgacgacatt tcgtgcgagt gtaccccaag
     6901 ttgcagcacc tattaacaga accaaagccg aatctttagg agtgcgcgtt gctgatgcct
     6961 ttgatacttt acaaacttat ttaggttctt cttatgtcaa tctgttcacg aaatttggtc
     7021 aagtatttcc agtctatgtt caggcggatg catcctcgag ggttagcagc gaagatttaa
     7081 ggaattacta tgtaagaaat caatccggct ccatggtgcc cttaggtact cttacagacg
     7141 tggggcctgc ggtaggaccc tcaattattt ctttatataa cctttatcca tcaagtaaca
     7201 taaatggggt tgctgcaaga ggttatagct cggggcaggg aatccaggtt atggagaaat
     7261 tggcaaagga acaattaccc ccagggatat cttatgaatg gacaagtaca gcttaccagg
     7321 aaaaggtggc tggtaacctc agttatttta tttttgcctt gtctttggtt ttggtttatt
     7381 taattttatc tggccagtat gaaaactggc ttattccatc tgcgattatc ctgagcgttc
     7441 ctttgacttt ggttggaaca gtattagctc tcggcagttt gggtatggat aataatatgt
     7501 acactcaaat tggcttgtta cttctcattg cattggctac aaaaaacgct attttgattg
     7561 ttgaggtagc gcgagaacaa cgtgaaattc ataataaatc agttcttgag gcagcggtca
     7621 ttggcgccaa aacacgtttt cgccctattc tgatgacatc ttttgcattt atcatggggg
     7681 ttatgccctt ggtttttgca acgggagcag gtgccaattc tcgtcgctca attggtatag
     7741 cggtgagcag tggtatgctt gcctcaactt gccttgcagt tgtttttgtg cctgtttttt
     7801 atgtattgtt gcaaacctgg caggacaaga ggaaggcaaa acattaatta atttaacgta
     7861 agttatggat aaagattaca taagaaccag tcgatatcca gaggacattt tggctgagga
     7921 gatgcttaag catcgtcttg aagccttggc atgaaacatt aaatcaaata cgagatttcg
     7981 agacggcata aatacctccg caacctgagt ggttcgagat aataactgat tttatccatg
     8041 actcatgcta atttacagct acagaagaat gctgtgagaa taattctaat cctctcacag
     8101 ccaaatagtg tatttatctt cctgaacatt caaacagttg atgctatcaa gctatattgt
     8161 atgcccaagg gtgtctgtag gaggctcatt tcttccagtg atttcactct tacctatttc
     8221 tttataactc ccgtgtacaa agtgtctgta ggaggctcat ttcttccagt gatttcaccc
     8281 atcgcttccc tgaattgtat tgatgtttgt ttagtagtag ggggttggac tgatttgatt
     8341 ggctcactaa gatttgattc ctcaagcttt tcagggttag tagaccattt tataatgtta
     8401 tttcttgtaa aatgctcggg ggcaattttg tcagccagtt gactgagttt ttcatgaaat
     8461 tgctcatcaa tcgctttttc ttcttttagt ttggagagta actgcgtatt gacctcttta
     8521 aacagtctgg gaatatcttg ttccaggaga gttttcttat cggttgattt attaatttcc
     8581 ctgtattcca ggcttaaata gctagctact tttgctgcct cttcagggtt caatgtctta
     8641 aagaattcaa ttgcttcatc attgtgacaa tgagaaggca agtccctgac aatttgttga
     8701 acagcaggat cttgtatctt ttgaaattgt tcttccattt ccagagagat gatttcatca
     8761 agttttgttg atttaaaggc atacttgtcc aattggcaaa tatctttcaa ttgatctcga
     8821 aataacctga cttccttttg cagagtactt tgaaaccaat tggattcggc agaaatgtct
     8881 ctgtcaattt tatctaatgt ttcaatagag tttaaaacct tttcaagaac ctggcttttc
     8941 gttaaagatt cactttgcag aagagtattg atttctttgg atactctttg caattcttca
     9001 cattgggtct tacgagcttc atttcttgaa cctttaaacc accattcgcg tcctctcttt
     9061 gcgttgtaaa tttttgcagt gagttctaat ccttcagcga ttttatttaa ttcaacatcc
     9121 aataatggct tggaggaata ttttgaccag gtaattgcac ttagattatc gtgttgtaaa
     9181 ttatcacgag aaagcaatct tgaaagatct tgtttttgac tctccgggat gctggtagcc
     9241 ttttccagct ctttttgaat ggctggccag ttacgattgt aagcaatgta attttgagtt
     9301 tccttcgaaa gcatggagta atttggaaca cctgtaggtg aggtagcaac caaataatcc
     9361 aataattctt ttggtttatt ctcagccagc agtttgacaa cctctttctt gaattgatcc
     9421 agtttttcgt acccctgggc tactacctca ttcttgtgat gtaagcgatt ggaggttcct
     9481 tcctggagag cttcctgatg acttgcaaga tcaccaagag gatgtttacc tttagtttgt
     9541 cgagtaaaga aatccagaaa atgacaactg attctgcctt gatgagaaaa gatattgcct
     9601 tttttatcgg gatcagcgac aaaaaactca tcaaaatgtg gagattcttt aatcgtatta
     9661 taatgttttg taaatttatc agtgatttct tgttgttgtt tgccggttaa cggagatttt
     9721 aatcccagct cggcatggtt gctgttaata tagtcgtata taattggttc tatatctgca
     9781 ccttgcgtaa atccttcctt tactcttttc gcaatttcag tagcatgagg ctctttgtcg
     9841 aaaaattctc caaagttagc atccgataat ttgtttgttt tacagtaaat attggcttcg
     9901 gccaacaggt actgaactct gatggatatc ttgtccgcat cgcggtctga tgatatttct
     9961 ttagcaccat catagaaagg gctggatgct tgagctaccc agacagtgga atctactgta
    10021 gcgtcgagaa tagtatcaat ccaatcacca atttcagagt cgttatctac aagcatcaaa
    10081 ctatttacta aataatcgta atttatttct tttcctcttg gatctttaga taaagatagt
    10141 ttcgaatatt gaggatcact ttttactaac tcttttacaa gttcgttttg aagattagta
    10201 attagttcgt ttaatttttc atcttttttt tctccatcaa ttctatcttc atctttataa
    10261 tgactaagaa cagtgctttt aactttatcc ctaagtgatt ttttattaat tggagttggt
    10321 gttttatctt ccgggaacag tgttgaacgc aggcgataac ctaatccctc gtatattgga
    10381 ataggagctt gccttgaggg tgtatcgtat ttagagatgt ttcttttgac attgaataaa
    10441 gggtcatcaa agcgagtaaa tttgtcgttg tcataaggag aaagtctcac agcaaaggca
    10501 ttttcagatg atttaatgat ttcctttact ccagaaggta attgtggaat ccctccagta
    10561 cgtagttgcc tgatatcatt tttttcatca tattgttttt ttagtacttg aattaattca
    10621 atgtattttt caatttgctg taagcgttct attttctctt ttaataaatc gtcataagca
    10681 tgaggtgaaa ttttcttttg gctattaatg gctttgatgt cttcttccaa ttgccttttg
    10741 tattcactaa gttggtgttc agcagaatat ttggtcacac cactgtgcgc gctaccatag
    10801 aaaaatgtaa tcaactcaac tgctgtttgg caggtgttat ctagacctat ctcatctcct
    10861 cctacaatcg gtaaaaggat atgaactttg ttgttttcat caatatatag gtagtcacaa
    10921 cgttccggct ctttgaattt gattacgtca accatgttta tcaccatttt ctagtgtctt
    10981 gtttataagt atattataaa gacaacttgt ttgtaaaaca acaaaaagat ttgtaattag
    11041 actttttgtt tgattgcaca tactccgcaa tctaatcata ttttttattc acaattgctt
    11101 gactccatac tgtcaagcta tatcgtgtgt ctaacggttt ctctgggtgg ctcatttctt
    11161 cctgtcattt catttatggt ttctctatat tttcttgcca tttcagaaag tgagctatca
    11221 gatgatttta ataattcgcc gagattggac tcctcaagct tttcaggatt ggcagaccat
    11281 tttctgatat tattccgtgt aaaatgctca ggaggaattt tatcagctag ttgagcaagt
    11341 ttttcataga ccccttctcc cagaacacta tcttctttta atttggataa taactgcatg
    11401 ttgacttctt taaacaaatt ggggatatcc tcgttcagga gcttagcctt atctgtggat
    11461 ttattaatct cacgatattc caggcttaaa taactggcta ctttagctgc ttcttcagga
    11521 ttcaatgtca taaagaattc aatcacttca ttattatgac aatgagaagg caagtcccgg
    11581 acaatttgtt ggacagcagg atctttaatc atttgaaatt gttcttccat ttccagagag
    11641 ataatttcat caagttttgt tgatttaaag gcatagttat ctaattgaca gatatctttc
    11701 aattgctctc gaaatgattg tacttctttt tgcagagtac tcttaaataa attatattca
    11761 gcagaaatgt ctctatcaat tttatccaat gcttctatgg agtttaaaac tttttcaaga
    11821 acttggcttt ttgttaacga ttcactttgc agaagagcat tgatttcttg agatactctt
    11881 tgcaattctt cacattgggt cttacgagcc tcatttcttg aacctttaaa ccattcactt
    11941 tttctttttt cattgtaaat tttagcagtt agatctaaac cttcggcgat tttatttaac
    12001 tccacatcca ataatggctt ggaagaatat tttgaccagg taattgcact gaggttctcg
    12061 tgttgcagat tattacgaga aagcaatctt acaagatctt gtttttggtt ctctggtatg
    12121 tcagtagttt tttcgagctc tgtttgaatg gctggccagt tacggttgta agcaatataa
    12181 ttttgagttt cctttgagag catggaataa ttaggagcgc ctgtggggga ggtagcaacc
    12241 aaataatcca ataattcttt tggtttactc tctgccagca gtttgacgac ttctttcttg
    12301 aattgatcca atttttcgta tccttgagcg actacctcat tcttgtgatg taaccgattg
    12361 gaggttcctg cctggagtgc ttcctgatga cctgcaagat caccaagggg atgtttgcct
    12421 ttggtttgtc gagcgaagaa atccaggaaa tggcaactca ttctgccttg atgggagaat
    12481 atattccctt ttttatcagg atcagcgaca aaaaattcat caaagtgagg agagttctca
    12541 ataatgtgat aacgttgagt aaatttttca gtaatttcct cttgttgctt gctgcttaac
    12601 tgagatgtta atcccagttc ggcatagtgg ctgttaatgt aattgtaaat aataggctca
    12661 atctctgctc cttgaacaag tccttctttc actcttttag caacttcagt agcatggggc
    12721 tctttatcaa aaaattcacc gaagttagca tctgataact tgttggtttt acaataaaaa
    12781 ttaatctcag ccaatagata ttggacttta attgacattg tatttgtatc agattcaaat
    12841 tttatttcct tttgcttctc atagaataca ctaacttttt cagtaccact ttcactttcc
    12901 ttttttggcc aataatcagc ttctgttgta gcaccaataa ttccataaac ccaatctttc
    12961 gcggtggagt tgtcatctaa cgtacatgct atgttttcca aataatccaa gttaagtggt
    13021 tggtgatagg tatcctcatc cacttgaagt tcggaatcaa ttttcttgta ctcttctaga
    13081 acgagttttt ttatatcctc aaatttttga ttacgatcgc catcttttgt attaaaatct
    13141 ttgtcaagtt gcgctaaaac agaatctacg attttgtcct tcagtgattt tttattaaaa
    13201 acaataggag tgtctttatc tggtggtaga agctcagaac gcaaacgagc ccctaatcca
    13261 tcagttgccc gttggtatcc cccagcttca tattgagatc tgtttctttt gagactaaac
    13321 agaggattat caaagcgggt aaatgagtct ggtctatccg gggaaagcct taaagcaaag
    13381 gcattttcag aagattgaat gacttccttt actccagaag gtaattgcgg aattccttct
    13441 gtgcgtagtt tgtctatttc tctttgctca tcaaatttct ctttgagtac tttgattaaa
    13501 tcaatatatt tttcgatttg ttccaggcgc tcttttttct cttttaatag atcttcatag
    13561 gcatttggtg aaatctttct ttgaacaccg attgccttta tatcatcttc cagattcttt
    13621 ttatattcat ttaggtgatg ttcagcagaa tactttgtcc caccatgcgt ttttccatag
    13681 aaaaaggcta ataactcacc tgttgtttca caggtattat ccagtcctat ttcatcccca
    13741 cctacaatag gtaaaagaat atggacttta ttatccttat cgatatgtaa atattcgcat
    13801 cgctccggct ctttgaattt tattttgtca accatgttca tcaccttatg ctagtgctct
    13861 aa
//
LOCUS       DBNCWK010000075        13852 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.075, whole genome shotgun sequence.
ACCESSION   DBNCWK010000075 DBNCWK010000000
VERSION     DBNCWK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.075"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            116..382
                     /locus_tag="AC06IO_001906"
     CDS             116..382
                     /locus_tag="AC06IO_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875084.1"
                     /translation="MESMIDLVGYVASSLVFATFYVKKILTLRLIAVCSNVAFITYGL
                     GYHLHPIFILHSVLLPLNLYRIFELLKNDPEFTILARINLKATR"
     gene            436..855
                     /locus_tag="AC06IO_001907"
     CDS             436..855
                     /locus_tag="AC06IO_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HIO9875085.1"
                     /translation="MCNWNRIIGLALMGFSAFLQAKTYIPQCPKEINTLERIQTSPDG
                     WETLSGIKNNYLGNVSFYSGHPKGQASLKPNRINKKKAEWKFSPHDTIYIVCHYNQTG
                     IELTQQLPQKTKGCTVIFNPNAKGPYGFLPQKITCNQ"
     gene            complement(865..2145)
                     /locus_tag="AC06IO_001908"
     CDS             complement(865..2145)
                     /locus_tag="AC06IO_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875086.1"
                     /translation="MNTLSFKHPLLAKTYLWIIWTIAACFFFYKYLIQVSPSVMTADL
                     MKAFQVNAAGLGNLSAFYFYSYLFMQIPVGVMLDRYSPRLLTTLAIFVCSISTYIFSQ
                     TNTLWLACLSRALMGAGAAFAAVSCFKLAAVWFSPKRFALVSGMFMTAAMLGAVGGQM
                     PLAFLVQHEGWRKALELVSVMGIILGVVYFLILRDKPAQTQPMPNSKEPVIQLLRRII
                     VNKQAWALSLYSGLAFAPVSVFGGLWGVPFLEKAYLLSRTDAALAISFIFVGFAAGAP
                     FWGWFSDFIRRRKPVLFTGTCSALLCLLVVIYSSNQNLLTLIILLFLFGFGASGFFTS
                     FAMIRELFPLVLVATVLGIMNTFNSVFEALFEPLVGALLDWTWEGTMVDGIHQFTLHG
                     YYFSLLLLPLSLILALLTLLLIDETYCRTKEEVV"
     gene            2622..3902
                     /locus_tag="AC06IO_001909"
     CDS             2622..3902
                     /locus_tag="AC06IO_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIO9875087.1"
                     /translation="MELTTTENQLFKSSKVSLALVTVLVGIGSGFLGMCLAMLLHYLQ
                     HIAYGYSPLHIISNETFLEGVRASSSERRISILFLCGLIAGMGWWALYRFGKPLVSIA
                     AAIKSGKPMPALSTFIHAFLQIITIALGSPLGREVAPREVSSVFAYWLSSKAGLTPEE
                     SKIMLACGAGAGLAAVYNVPLGGAVFVLEVLLCTSNWTVLLPALSSSAIAVLVSWWGL
                     GNESIYQLPEFSLSAPIILWSILASPVLGFFAFWFIQIATIQRSKAMHNRQMILACLI
                     NFLIIGLFAIYFPSLLGNGKSPAEMEFDQSVGIGLSIVLFMLRNVFVWSSLRVGAQGG
                     LLTPSLANGALLGAILGGIWNLYLPPAPIHAFALIGATAFLAAAQKMPLTAIILIFEF
                     TRINFIFLIPIMLAVSGSIGMCQLTKNYYIKYKV"
     gene            complement(4032..4280)
                     /locus_tag="AC06IO_001910"
     CDS             complement(4032..4280)
                     /locus_tag="AC06IO_001910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875088.1"
                     /translation="MPWVIQRKIKQINTLAEQYIEDIRYFLTEGVVAPTKALSFHFNG
                     VACFTKGKLGKKYQFGRCIQLGRIDGNFLLVTNQRLFI"
     gene            complement(4286..5395)
                     /locus_tag="AC06IO_001911"
     CDS             complement(4286..5395)
                     /locus_tag="AC06IO_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875089.1"
                     /translation="MFKKKHMVKDDSDMDYVDDFISIEEHNSRDDVTDELSLAEQVFT
                     RLQIYSEGFKPSFFINKDLANLSLTSKTLNDVVCSELNQRAAQKLLTHVVKGEQDKAQ
                     AMIEKNPGLLLIKSKAVDYSGRTIVGTAFQAAIGSGDKPMWEMILPYFECLEQGEALR
                     QFQGQFPSGIEETPAAELKAYYDAIALAIIHDEDHGLSVIERFRKKITCQKEITQGKH
                     FNLQHLIAVYQAYIDNFHALGNWNNRDLFWQKVIGYVQRQMTAYDAQIHCSGVVRCFL
                     DKESSFSRTFELSNDKELFPLQIDSGLGFDFACYSYYSARPLDSGSHNVLPSPLNEYW
                     IWIEKLCGEKRMHLLDLESRLSKGCIISSNNYSLL"
     gene            5597..6100
                     /locus_tag="AC06IO_001912"
     CDS             5597..6100
                     /locus_tag="AC06IO_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875090.1"
                     /translation="MPKKFTRKIPLISREECFALLPGYSINAMNLLKRGNHTLYYEEI
                     ESLSKIFVALVTHYSEPIESIINCMEKAMYGAYKEELDKIEHEIQRLFKIRKNTPIHS
                     LFGEREIEKLTIEISELEKRKSVPVKEIIQGVIYDTLDKASQTLVDRIPANYKLQEYE
                     SRGNPFQ"
     gene            6367..6846
                     /locus_tag="AC06IO_001913"
     CDS             6367..6846
                     /locus_tag="AC06IO_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HIO9875091.1"
                     /translation="MTLCPCGSQNKYEQCCGIYLESKKTPETPEQLMRSRYTAYSLAK
                     IDYIKQTMQGKPLIAFNESEAKQWAQNVSWLGLKIIQSYMERPDKGFVEFIATFLDGN
                     QLKNIHELSEFQKNNSVWFYVDGEAKQLPNKQDKQKIGRNNPCPCGSGKKFKNCHDK"
     gene            6866..8671
                     /locus_tag="AC06IO_001914"
     CDS             6866..8671
                     /locus_tag="AC06IO_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HIO9875092.1"
                     /translation="MNGHLLLNVFIFLAAASIMVPLASRFRLGSVIGYLTVGMLIGPF
                     GLKLIGNAEQIMHFAEFGVVMMLFLIGLELEPIMLWKLRKLIVGLGGLQVLLTTIALS
                     SAAVMMGYNLRASVAVSMALALSSTALVLQMLQEKNLLKTAEGETSFAVLLFQDIAVI
                     PILVILPLLGNHHGINVAINPHTAASIAQLPKWLHAIVVAGIIGSIVLAGHYLSRYLF
                     LIIAKTNIREVFTAFSLSLVVGITLLMETIGVSPALGAFIAGMVLANSQYKRTVEADI
                     QPFKGLLLGLFFISVGMGINFNLLFSQMAVIIPIVLGLITIKALILFTLGKIFQLTKI
                     QALGFALGLSQGGEFAFVLFQYSSTLKVISPEISSFFTLVVALSMLATPFLMLLYHRF
                     MMPKLISKLPEREYDSIHEKNNIIIAGYGRFGQIIGRFLSGENIKVTVLEKNPEQVEL
                     LRKFGYTGYFGDANRLDLLKSAGAAHAQLLIVTVGNPDTNLEIVKLAKEEFPQLKIFA
                     RARNRRHAYELHKAGVHYFKRELFDSSLTMTKEILKFLGYKPEEIEKKAKAFQKHDEE
                     TLIKSFDFFEEEANLINFSRQAKGELERILQNNNP"
     gene            8725..9627
                     /locus_tag="AC06IO_001915"
     CDS             8725..9627
                     /locus_tag="AC06IO_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIO9875093.1"
                     /translation="MIKQLQQALSDILLGKEEVIRLGITCLLANGHLLLEDNPGMGKT
                     TFSHALAQLTGMQYRRIQFTSDLLPSDLLGVSIYDHQRNVFQFHKGPIFSQLILADEI
                     NRATPKTQSALLEAMEERQVTTDIGTEALPQPFFVIATQNPSCHTGTYPLPESQLDRF
                     LMRLVIGYPPPDAERMLLKSSNLNAQLKELSPIINPQQLQILQRESQKVLVSELVLDY
                     VQSLLDASRNQGWFNHGLSPRAGMGLVHAAQAFARTDDRDFVRPDDIQAVMPAVVNHR
                     LIIKKSQTQLSAAELLMSEIEVPV"
     gene            9624..10574
                     /locus_tag="AC06IO_001916"
     CDS             9624..10574
                     /locus_tag="AC06IO_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875094.1"
                     /translation="MIRQIKTKVMTYWEEWIAKRISKTNPQILDSQNIYIMPSGFGWA
                     YSVVVLSLFSGAINYQVSTVFLMSFILAIVGFISAWEAHANLKGLSIKLVSVEDTYEG
                     TPAQVSLLIQSNNRIHFGLDFHLNNQSVTRLEKIPPQGLRFILPLATTRRGCYTLPKI
                     TISSFFPFGLFQVWGYANFDSNYYVYPQPVNPGFWPTPFYHQNKKKTDSQGDNEYYDL
                     KQVENPWIQPNLIAWKIAAKGQGWYLKTMDRAEEMYWLFRLNDLPIETLEEKLKHMSY
                     WLVTAESQGQFYKLELGNDPDQFSHGEEHLRYCLRQLAVY"
     gene            10574..12562
                     /locus_tag="AC06IO_001917"
     CDS             10574..12562
                     /locus_tag="AC06IO_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HIO9875095.1"
                     /translation="MDFFPKQHIFLITTRYALLVMLICYFPHFLTCPLWLSIFILTTL
                     CYKLISDYFAYPLLDSKIRFIVVIICLFLLKFQYGSIISSGFFIGFLLAFIGLKTIEI
                     HNDRDIRILILCNFYLIFAALIVVQELWIIPYLLIAILANFTLMLKLTAPQASLRQIS
                     SRSMKLLLIATPLTVFLFYFFPRIANPLWQVPSLGKSYTGFSEWMEPGSVANLLNDDR
                     TALRIIFKRKAILNGYWRGLTLSRYNGISWNPAWHDSSTFSPLKELTTSDDGDYEVIM
                     EPHQKKWLFYLGYPNAAYPQLLFSPNFGLVSHDKDMIAQRFAYGLRISSSPHSSLTQK
                     DWKLNTQLPKYSNPRITFWAKKQFAEVNYDPQTFIQFIQNYIKKESFWYTLNPPKLDN
                     ENDQMDYFWFDSRKGFCEHYASAVTVVLRAADIPARVIVGYQGGEWNPLAKYLTIKQY
                     DAHAWVEYWQKDFGWKQLDPTAFISPSRIDPSVLAMRAARTNQQLIIDPSNLNWIQKI
                     KLYMESTRFFIERWLLFYNQDAQYKLLEQIGLKHWNAAMLLQIAILSLVLFIILLGAC
                     YQWRQKRTLDPLLREYHLLQKEFCRFNIATQPPATLNKQCKILINKIPNLEETITTFL
                     SHYEKLRLRHRITKTKENKKQTLLLFKNLRRILKQVKI"
     gene            12644..13831
                     /locus_tag="AC06IO_001918"
     CDS             12644..13831
                     /locus_tag="AC06IO_001918"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIO9875096.1"
                     /translation="MVHFQKMTVQTFLKDYWQKKPLIIRQALPDFTNPLTPDELAGLA
                     LEEEIESRLVYETPDQSPQWNLKRGPFKESDLIGLPKTHWTLLVQGVDRIVPDVYELL
                     DHFNFIPQWRVDDVMISYATLHGSVGPHYDNYDVFLYQAKGRRLWSLTSKKCHTNNFI
                     KGLELRIMKEFEVEEQFILEEGDMLYLPPHIGHYGIAQSEECMTYSFGYRSYQGQELW
                     DSLGDYLSEHDLFKSLYQDPDWSTLKNTSEITPKAWDNARQLLRQVLENDQLMHSWFG
                     CFATSLDQSAEQYLPPPLEEDELLGLDEFIKELANYQKIVRDASCRFAYIMSDQESQC
                     HFYVNGKKWDSRGVSTNLLSFVANNRFLPLKELKPYLNHKTNQLFLYELWKLQWLQIS
                     HDQ"
BASE COUNT         4383 a         2738 c         2528 g         4203 t
ORIGIN      
        1 tcatctatta tacatttcaa cgcttaagaa aaccataaga attgattaaa aaacttgcta
       61 atatcctctg gtcagtgtta tgttgaatga aagggatttg gaagtggagg ttaaaatgga
      121 atcaatgatt gatttggttg gatatgtagc ttcttcttta gtctttgcaa cattttatgt
      181 caaaaaaatt ctgacattaa gactcatagc cgtttgtagt aatgttgcgt tcattactta
      241 cggtttgggt tatcatttac atcctatatt tattttgcat tcggttttat tgcctttaaa
      301 tttatacaga atatttgaat tattaaaaaa tgatccagag ttcacaatac ttgcaaggat
      361 caatttgaag gcaaccagat aatcattttt atcatttaat gatatgatag ctgtttactc
      421 aataaggggt cttccgtgtg caattggaac aggataattg gcttggcatt aatgggattt
      481 tctgcttttt tacaagctaa aacctatatc cctcaatgcc ctaaagaaat taatacgctt
      541 gaacgtatac agacatctcc ggatggatgg gaaaccctga gcgggattaa aaacaattat
      601 ctgggtaatg tttcctttta ttccggccac cccaaaggtc aagccagctt aaaaccaaac
      661 cgtatcaata agaaaaaagc cgaatggaag ttttctcctc atgataccat ttacattgtg
      721 tgtcattaca atcaaacagg aattgaactg acacaacaat tgcctcaaaa aacaaaagga
      781 tgtactgtga tttttaatcc aaacgcaaaa ggcccatatg gtttcctgcc tcaaaaaata
      841 acctgcaatc aataataagg aggtttacac tacctcctcc tttgttcgac aataggtttc
      901 atcaatcaat aacaaggtca ataaggctaa aattaaagat agaggcaaca ataaaagaga
      961 aaaatagtac ccgtgtaaag taaattgatg aataccatcc accatggtcc cctcccatgt
     1021 ccaatccagc aaagcaccaa ccagtggctc aaacaaagcc tcaaacaccg aattaaacgt
     1081 attcataata ccaagtactg tcgctacaag taccaaagga aataattccc tgatcattgc
     1141 aaagctggta aaaaatccac tcgctccaaa accaaagaga aataaaagaa taattaaagt
     1201 aagcaaattt tggtttgagc tataaataac taccaataaa cacaatagag cagaacacgt
     1261 tcctgtaaac aaaacaggct ttctccgcct gataaaatca gaaaaccatc cccaaaatgg
     1321 cgcgcctgcc gcaaaaccga caaaaataaa tgaaatcgca agggctgcat cagtacgaga
     1381 taagagatag gccttttcca aaaaaggaac tccccataat ccaccaaaaa ccgaaacagg
     1441 agcaaatgct aatccactat ataaagacaa agcccaggcc tgcttattaa cgataatacg
     1501 tcttaacaat tgaataacag gttccttgct atttggcata ggctgcgttt gtgcaggctt
     1561 atctcttaaa atgagaaaat acacgacccc caggataatc cccatcacgc taactaattc
     1621 aagcgccttg cgccaacctt catgttgaac caaaaatgcc aaaggcatct gccctcccac
     1681 agcacccagc atagctgctg tcataaacat ccctgatact agagcaaatc gttttggaga
     1741 aaaccagaca gcggctagct taaaacaaga cacagcagca aaagccgctc ccgcccccat
     1801 caatgctcga cttaggcagg ccaaccataa agtattggtt tgggaaaaaa tataagtact
     1861 gatactacag acaaaaatag caagggtcgt cagtaatctt ggactatacc tgtctaacat
     1921 aacacccaca ggaatctgca taaataaata ggaataaaaa taaaaggctg acaaattccc
     1981 aagaccagcc gcattaacct gaaatgcctt catcaagtca gctgtcataa cactgggaga
     2041 aacctgaatc aaatatttat aaaagaaaaa acatgccgcg atggtccata taatccaaag
     2101 ataagtttta gccagcaatg gatgcttgaa tgacaatgtg ttcacaataa ctcctaactt
     2161 atttgcttca ggttagagat aaaatttcaa aaaccgccag gtcttatctg ctgctgttat
     2221 cgccctgagg cggggcagat aagtcaggag agaagccgtc tcagagcaga agaaagggta
     2281 aagctgacca ccaccacgac gacaattgag atgagagagt ttaaaacaac aggcgacaat
     2341 ccaacaggct gtctgttaca gctttgggaa caagactttc tgcttattaa ttgtcgtctt
     2401 atattcataa aaaactggaa attaaaagaa tgtttaatgt taacttttta ggcaatgtgc
     2461 tgtcaagatc gcaaacaaac aatggatatt tttttaagtt ttgctattac ggctacattt
     2521 attaatctgt ttcacttgca aaaaaatctg catacagaaa taataaaatt cttaccccta
     2581 taagcaaatt gatgacaagt taccttaaag gaatccaggg aatggagtta accacaaccg
     2641 agaaccaact atttaaaagt tccaaagtaa gtcttgctct ggttactgtt ctggtaggaa
     2701 tcggttcagg ttttctaggc atgtgcctgg ccatgttatt gcattatctt caacatattg
     2761 cctatggtta cagccccctt cacatcatca gtaatgaaac ttttctggag ggagttagag
     2821 cctcttcatc tgagcggaga ataagcatac tattcctttg tggcttaatt gcaggtatgg
     2881 gatggtgggc actttatcgc tttggtaaac cattagtcag tattgcggcg gcaataaaat
     2941 cgggtaaacc aatgccagcg ctcagtacat tcattcatgc ttttttacaa atcataacaa
     3001 ttgcattggg ctcacctctg ggacgagagg tggctcctcg tgaggtaagc tctgtctttg
     3061 cttactggct atctagcaaa gcaggattaa ctcctgagga aagcaagatc atgttggcat
     3121 gtggagcagg cgcggggctt gctgcagttt ataatgtgcc tttaggcggc gccgtttttg
     3181 ttttggaagt gttgttatgc acttccaact ggactgtctt gcttcctgca ttatcttctt
     3241 ctgctatagc agttctggtg tcttggtggg ggttgggtaa tgaatcaatt tatcaactcc
     3301 ctgaattttc attaagtgca cctataattc tttggtctat tcttgccagc ccggtgttag
     3361 gtttttttgc tttttggttt attcaaattg ctacaataca acgcagcaaa gcaatgcata
     3421 accggcaaat gatattggct tgtttaatta attttttaat cattggctta ttcgcaattt
     3481 attttccttc tttactaggt aacggcaaaa gtccggcaga aatggagttt gatcaatcag
     3541 taggaattgg tttaagtatt gtattattta tgttgaggaa tgtattcgta tggtcaagct
     3601 tgcgtgtagg agcacaaggt ggcttactaa ccccctcact ggctaacggc gccttattag
     3661 gagctattct aggtggaatt tggaatttgt atttaccacc cgctcccatc catgccttcg
     3721 cgcttattgg tgccacggct tttcttgctg cggcacaaaa aatgcctttg actgccatta
     3781 tcctcatttt cgaattcacc cggattaatt ttatctttct tatccctatc atgcttgctg
     3841 tttccggctc tataggtatg tgccagttaa ccaagaacta ttatattaaa tataaagtat
     3901 gattctttgt aacaagtatt tttcattggc tgcactgtaa tatcccttat cagttccaac
     3961 attatttatt gatacaccac caaaaagctc ctggtggcat tcaataacag caggaagaga
     4021 ggttttatca actatatgaa cagacgttga ttggtaacta acaaaaaatt tccatcaatg
     4081 cgtccgagtt gaatgcatcg tccaaattga tacttttttc caagtttccc ttttgtaaaa
     4141 caagcaactc cattgaaatg aaatgaaagt gcttttgttg gggctactac tccttcagtc
     4201 agaaaataac gaatatcttc aatatactgc tctgccaatg tattgatttg ttttatttta
     4261 cgttgaataa cccagggcac tttggtcaca ataaagaata attatttgag ctgataatac
     4321 accccttgct caagcgactc tccaagtcca gcaagtgcat ccgtttttct ccacataatt
     4381 tttcaatcca tatccagtat tcattcaaag gggaagggag cacgttgtgg ctaccggaat
     4441 ccaaaggccg ggccgaataa taactataac aagcaaaatc gaaaccgaga ccagaatcaa
     4501 tctgtaaggg gaacaattcc ttatcattag acaactcaaa cgtcctactg aatgaactct
     4561 ctttatctaa gaagcacctt acaacacccg aacaatgaat ttgggcgtca taagcggtca
     4621 tctgccgctg cacataacca atcacttttt gccaaaataa gtcacggttg ttccaattac
     4681 ccaaagcatg aaagttatca atataggcct gatacactgc aattaagtgc tgcagattaa
     4741 aatgcttgcc ttgcgtaatt tccttttggc aggttatttt ttttctaaat ctttcaatca
     4801 cggagagacc atggtcttca tcatgaataa tggctaatgc aatagcatcg taatatgctt
     4861 ttagctcagc ggcaggagtt tcttcaatcc cactgggaaa ctgtccctga aactgtctta
     4921 aggcttctcc ttgctctaag cactcaaaat aaggaagtat catctcccac atgggcttat
     4981 ctcctgaacc aatcgctgct tgaaatgctg ttcccacaat ggttcttcca gaatagtcta
     5041 ccgcttttga tttaattaag agcaatcctg gatttttttc tatcatcgct tgggctttat
     5101 cttgttctcc cttaactacg tgggttaaga gtttctgagc cgctcgttga ttcaactcgg
     5161 aacatacaac atcattcaat gtcttagagg ttaaactaag attcgctaaa tccttgttta
     5221 taaaaaagct cggcttaaat ccctctgagt agatttgcaa tctggtaaat acttgttctg
     5281 ctaaagaaag ttcatcagta acatcatctc ttgaattgtg ttcttcaata cttataaaat
     5341 cgtcgacata atccatgtct gaatcatctt ttaccatatg cttttttttg aacacatcgt
     5401 ttcactttac tagactatta aagagggcag agtttatcac aaaataatcg atatggtcat
     5461 taatcatact tggtaatggt tgtttcgtgc cagcgactaa ccatctgcaa tctgactaat
     5521 tgaagtacaa tatgatcaaa ttaaataata tagatgctat aattataatg tagacctgat
     5581 gaaaaggatg ttcactatgc ctaagaaatt tactcgaaaa ataccactta tttccagaga
     5641 agagtgtttc gctttattac ctgggtacag catcaatgca atgaatcttc tgaaacgagg
     5701 gaatcacaca ctctattatg aagaaattga gtccttatca aaaatttttg ttgccctggt
     5761 cactcattat tcagaaccaa tagaatcaat catcaattgc atggaaaaag caatgtatgg
     5821 tgcttataaa gaggaattag ataaaattga gcacgaaatt caacgcctgt ttaagataag
     5881 aaaaaatact ccaattcatt ctctatttgg cgaaagagaa attgaaaaat tgactataga
     5941 gatttcagaa ttggaaaaac gcaaatcagt acctgtaaaa gaaattatcc agggggttat
     6001 ctacgatact ttagataagg caagccaaac tctggtggat agaataccag caaactataa
     6061 actgcaagaa tatgagagcc gaggcaatcc ttttcaataa gattcaggta cattgttaaa
     6121 tgaaacacaa tcaatcactt ataacctaat ttactctgat agcgcttggt catttctttt
     6181 tatcacccat tttaaaatcg ctgtgtggat ttatagtgaa tcacactttg tccctgtttt
     6241 tataaaaaaa atccaatgca ataaaactgt ttagaaataa tggaatgcca tatagaatat
     6301 aatccgggat tcgttaatat aacggattgt aaatttaccc ataaaccagt ttttacaggt
     6361 aactaaatga ctctttgccc ctgtggttca caaaataaat atgagcaatg ttgcgggatt
     6421 tatctggaaa gcaagaaaac tccggaaaca ccagaacaat taatgcgttc gagatatact
     6481 gcgtattctc ttgcaaaaat cgattatatt aaacagacca tgcaaggcaa accactaatt
     6541 gctttcaacg aatcagaagc aaagcagtgg gctcaaaatg tttcctggct gggtttgaaa
     6601 atcattcaaa gctacatgga gaggccggat aaaggatttg ttgaatttat agcaaccttt
     6661 cttgatggaa atcaattgaa aaatattcat gaattaagtg agtttcaaaa gaataattcc
     6721 gtttggtttt atgtggatgg ggaagctaaa cagttaccaa acaaacaaga taagcaaaag
     6781 ataggccgca ataacccatg tccctgtgga agcggtaaaa aatttaaaaa ctgtcatgat
     6841 aaatgaatta aactcaaggt agcagatgaa cggtcactta ctcctcaatg ttttcatttt
     6901 ccttgctgct gcaagcatca tggttccact ggcaagccga tttagacttg gttctgtgat
     6961 tggttatctt accgttggga tgctcatcgg accttttgga cttaagctaa taggcaatgc
     7021 cgaacaaatt atgcattttg cagaatttgg agtagttatg atgttattct tgattggttt
     7081 ggaattagaa ccaatcatgc tctggaagct gagaaaatta atagttggct tagggggctt
     7141 gcaagtgttg ttaacaacaa tcgctctgtc ttcagcagct gttatgatgg gatacaattt
     7201 gcgagccagt gtagcggtca gtatggcact tgccctatct tcaactgctt tagtcttgca
     7261 aatgctccag gagaaaaatc ttttgaaaac cgcggagggt gaaacttctt ttgctgttct
     7321 cttattccag gatattgcgg tcataccaat cttggtaatt ttgcctttat tgggcaatca
     7381 tcatggaatt aatgtggcta taaaccctca tacggctgcc tctattgctc aattacccaa
     7441 gtggcttcac gctattgtcg ttgccggcat tatcggtagc attgttttgg cgggacacta
     7501 cttatccaga tacttgttcc taatcattgc aaaaaccaat atccgtgaag tatttacagc
     7561 attttcccta tccttggtcg ttggaattac cttgttaatg gaaacaattg gggtgtctcc
     7621 ggctttggga gcttttatcg caggtatggt tttagctaac tctcaatata aacgaactgt
     7681 agaagctgat atccaacctt tcaaaggact gcttcttgga ttatttttca tctcagtcgg
     7741 aatgggtatt aacttcaatc tactcttcag tcaaatggcc gtaatcatcc ctattgtact
     7801 cggtttgatt actattaaag cattgattct ctttacgctc ggcaagatat ttcaactcac
     7861 taaaatacag gcgctcggat tcgcccttgg tttatcacaa ggtggagaat ttgcctttgt
     7921 tctttttcaa tactccagca cattaaaagt aatcagcccg gaaataagca gttttttcac
     7981 tctggtagtc gccctgtcca tgttggccac acccttttta atgcttttgt accatcgttt
     8041 tatgatgccg aaattaataa gcaaattacc agaacgtgaa tacgactcta ttcatgaaaa
     8101 aaataacatt attatcgctg gttacggtcg atttggtcag ataattggtc gatttcttag
     8161 tggtgaaaac atcaaggtaa ccgttttgga aaaaaatccg gagcaagtcg aattattaag
     8221 aaaatttggc tacaccggat attttggtga tgcgaatcgt ctggacctgc taaaaagcgc
     8281 aggagcagcg catgctcagt tgctcatagt cacagtcggg aatcccgaca ccaatttgga
     8341 aattgtgaaa ttagctaaag aagagtttcc tcaattgaaa atttttgccc gagcaagaaa
     8401 tagacgacac gcctatgagt tacataaagc aggagttcat tatttcaaaa gagaattatt
     8461 tgactcttcg ttaaccatga ccaaggaaat tttaaaattt cttggttata aacctgaaga
     8521 aattgagaaa aaagccaaag ctttccaaaa acacgatgaa gaaacattaa ttaaatcttt
     8581 tgattttttt gaggaagaag ccaatctcat caatttctca cgtcaggcaa aaggagaatt
     8641 agaacgtatt ttgcaaaata ataatccata atcaaatcat aaaggtctat acttccatat
     8701 aatcatcttg aaatggcttt cattatgata aaacaattac aacaggcatt aagtgatatt
     8761 cttttaggaa aagaagaagt catcagattg ggtattacct gtttattagc caatggccac
     8821 ctgcttcttg aagacaaccc aggtatgggg aaaacaacat ttagtcatgc gctggctcaa
     8881 ctgactggta tgcaatatag gcgaatacaa tttaccagcg accttttgcc cagtgatctt
     8941 ttgggagtat ctatttatga tcatcaacgc aatgtctttc aatttcataa gggtcctata
     9001 ttctctcaat taattctcgc tgacgaaata aacagggcca cacctaagac tcaaagcgcc
     9061 ttattagagg caatggaaga gaggcaagtt actactgata taggaacaga agctctccct
     9121 caaccatttt ttgttattgc tactcaaaat ccgtcttgcc atactggaac ttatcctttg
     9181 cctgaatcgc aattggatcg atttttgatg cgacttgtca ttggctaccc accgccagat
     9241 gcggaacgta tgcttttaaa atcatcaaac ttgaatgctc aattaaagga gctaagccca
     9301 atcattaatc cacagcaatt acaaatttta caaagagaaa gtcaaaaagt tctagtctct
     9361 gaattggttc ttgattatgt tcaatctcta ctggatgcca gtagaaacca gggatggttt
     9421 aatcatggat taagcccaag agcaggaatg ggattagtgc acgccgctca agcctttgcc
     9481 agaactgatg atagagattt tgttcgacct gatgatattc aggcagtcat gccagcagtt
     9541 gtgaatcacc gcttaatcat aaaaaaatca caaactcaac tctctgctgc cgagttatta
     9601 atgagtgaaa ttgaggttcc cgtgtgataa ggcaaatcaa aacaaaagtt atgacttatt
     9661 gggaagaatg gattgccaaa cgaatctcta aaaccaatcc ccaaattctt gattcacaaa
     9721 atatttatat tatgccctct ggatttggat gggcctatag tgtggttgtg ctgtccctgt
     9781 tttctggcgc aatcaactat caagttagca ctgttttttt aatgagtttc attttagcaa
     9841 ttgtaggttt tataagtgct tgggaagctc atgctaatct taaaggatta tctattaaat
     9901 tggtatcggt tgaggatacc tatgaaggaa cgccagccca ggttagctta ttgatccaat
     9961 caaataacag aatacatttt ggactagatt ttcaccttaa taatcaatca gtcactcgat
    10021 tagaaaaaat tcctccgcaa ggtttgcgat ttattttacc tctggcaaca actcgaagag
    10081 gatgttatac cctgcccaaa atcactattt ccagtttttt tccttttggc ttgtttcagg
    10141 tatggggata cgccaatttt gatagcaatt attatgtcta tcctcaacct gtcaatcctg
    10201 gcttttggcc cacacccttt tatcatcaga ataaaaagaa aacagactct caaggagata
    10261 atgaatacta tgacttaaag caggtagaaa atccctggat tcaacctaat cttattgcat
    10321 ggaaaattgc agcaaaagga caaggttggt atttgaaaac gatggaccgc gctgaagaaa
    10381 tgtattggct ttttagatta aatgatttac ctattgaaac actcgaagaa aaattaaaac
    10441 atatgagcta ttggttggtt actgccgaat cacaaggcca gttttacaaa ctcgagttgg
    10501 gaaacgatcc ggatcaattt tcccatgggg aagaacatct tcgatattgc ttacgtcaat
    10561 tggcggtgta ttgatggact tttttcccaa acaacatatt tttctgataa caactcgata
    10621 cgctcttctg gtcatgttga tatgctactt tcctcatttt ttaacatgcc ctttgtggct
    10681 tagtatcttc atcttgacaa cgctatgtta caagttaata tccgattact ttgcctatcc
    10741 acttcttgac agcaagatac gctttattgt ggttatcatc tgtctttttc tattaaaatt
    10801 tcaatacggt agcattatct ccagtggttt ttttattggt tttttactgg catttattgg
    10861 attaaaaact attgaaattc ataatgacag agatataaga atactaatac tgtgcaattt
    10921 ttatcttatc tttgccgcat taattgtggt tcaggaacta tggatcatac cttatttatt
    10981 aatagcaatt ctggctaatt tcactctgat gctaaaatta actgctcctc aagcctcact
    11041 aagacaaatt agcagccgta gtatgaaact attactgatt gcaacaccat taactgtctt
    11101 tcttttctac tttttcccac gcatagccaa tcccctatgg caagtaccgt cccttgggaa
    11161 aagttacact ggatttagcg aatggatgga accagggtcg gtcgcaaatc ttctaaatga
    11221 tgacaggact gctttacgaa ttatatttaa aagaaaagcc attctaaatg gctattggcg
    11281 aggattaact ttaagtagat acaacgggat aagctggaat cctgcctggc atgactcatc
    11341 aaccttctct ccattaaaag aattaactac ttctgatgat ggggattatg aggtcattat
    11401 ggagccccac caaaaaaagt ggttgtttta tcttggttac cccaatgcgg cttaccctca
    11461 actcttattt tctccgaatt ttggtttggt tagtcatgat aaagacatga ttgcccagcg
    11521 ttttgcctat ggtttgcgaa ttagctcttc ccctcactcc tcattgacgc agaaagattg
    11581 gaaacttaat actcaattac ctaaatattc aaatcccaga attacgttct gggcaaaaaa
    11641 acaatttgcc gaagtaaatt atgaccccca aacctttatt caattcatcc agaattacat
    11701 aaaaaaggaa tccttctggt ataccctgaa tcctccaaaa ctggataatg aaaatgacca
    11761 aatggattat ttctggtttg attccagaaa aggattttgt gaacattatg caagcgcagt
    11821 cactgttgta ttacgcgctg ctgatatccc tgcccgggtt attgtcggct atcaaggagg
    11881 tgaatggaat ccactggcaa aatatttaac tataaaacaa tatgatgcac atgcctgggt
    11941 agagtattgg caaaaagatt tcggctggaa gcaactggat cccactgcat ttatttcccc
    12001 ttcgcgtatc gatccttctg ttcttgccat gcgagcggca cgcaccaatc aacaattaat
    12061 aattgatccc tcaaacttaa actggattca gaaaatcaaa ttatatatgg agtcaacccg
    12121 tttttttatc gagcgttggc tgttatttta caatcaggat gctcagtata aattattaga
    12181 acaaatcgga ttaaaacatt ggaatgccgc catgctattg caaattgcca tattgtctct
    12241 ggttttattt atcattctct tgggtgcctg ttatcaatgg cggcaaaaaa ggacattaga
    12301 tcccttattg cgtgaatacc atcttttaca aaaagaattt tgccgcttca atattgcaac
    12361 tcaaccgccc gccacgttga ataaacagtg taaaatatta atcaataaaa ttcccaatct
    12421 tgaagaaacc atcactacct tcctttctca ctatgaaaaa ctgcgtttgc gacatcgcat
    12481 taccaaaact aaagaaaata aaaaacaaac gcttttgttg tttaaaaatc tgagacgaat
    12541 attaaaacag gtaaaaatct aaatttgacg ggatttatcg gttgcattct tgagtctcaa
    12601 agtgcttaaa tagataacat ttatcaaact atgattttta ataatggttc actttcaaaa
    12661 aatgactgtc caaacctttt taaaagacta ttggcaaaaa aaaccattga tcattcgtca
    12721 ggctttacct gattttacta atccactcac accggatgag ttggcaggac ttgctcttga
    12781 ggaagaaata gaaagccgcc tggtctatga aacgcccgat cagtccccgc agtggaattt
    12841 aaaaagaggc ccgtttaagg aatctgattt gataggctta cctaaaactc actggaccct
    12901 attagtacaa ggagtagacc gcattgttcc agatgtctat gagttacttg atcatttcaa
    12961 ttttatccct caatggcgag ttgatgacgt gatgattagt tatgccactc tacatggaag
    13021 tgtgggccca cattacgata attatgatgt tttcttgtat caggctaaag gtcggcgact
    13081 atggtcatta acctcaaaaa aatgccacac caacaatttt ataaaaggtc ttgaactacg
    13141 aatcatgaaa gaatttgagg tagaagagca atttatcctg gaagaaggcg acatgctcta
    13201 tttacccccc catataggac attatggaat cgctcaatct gaagaatgta tgacttactc
    13261 ttttggttat agaagttacc agggacaaga gttatgggat agtctgggtg attatttgtc
    13321 tgaacatgac ttgttcaaat ccctctatca ggatccagat tggtccactc ttaaaaacac
    13381 atcagaaata acgcccaaag cttgggacaa tgcaaggcaa ttgctaagac aagtcctgga
    13441 aaatgatcag ctcatgcact cctggttcgg ttgttttgct acaagccttg atcaatcagc
    13501 agaacaatat ttaccccctc ctttagaaga agatgaatta ctcggtttgg acgaatttat
    13561 taaagaattg gctaattatc agaaaatagt tagagatgcc tcatgtcgtt ttgcttatat
    13621 catgtctgac caagaatccc agtgccattt ttatgtcaat gggaaaaaat gggacagccg
    13681 cggggtttca acaaacttac tcagttttgt tgccaataat cgattcctgc ctctaaaaga
    13741 gttaaaacct tatttaaacc ataagactaa tcaactcttc ctctatgaat tatggaaatt
    13801 acaatggtta caaatttctc atgatcaatg atttggttta taaatatctt ga
//
LOCUS       DBNCWK010000076        13714 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.076, whole genome shotgun sequence.
ACCESSION   DBNCWK010000076 DBNCWK010000000
VERSION     DBNCWK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13714
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.076"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            68..1957
                     /locus_tag="AC06IO_001919"
     CDS             68..1957
                     /locus_tag="AC06IO_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIO9875097.1"
                     /translation="MPSTRNIETHNDSNPTLRALSLSALGIVYGDIGTSPLYTFKTVI
                     LLAGGGTPDVTTIMGSVSLIIWTLIIIASVKYISFALRIDNDGEGGILALMSLLSLKL
                     KQKPFIIAVGLMGAALIYGDGTITPAISVLSAVEGLEILSPSLKYYVLPIAITILITL
                     FAIQSKGTATIGKAFGPVMAFWFLTIGILGARGVIQHPFVLAAINPVYGLNFLFSNGA
                     TGFFILCGVFLCVTGAEALYADLGHFGTAPIRCAWFGLAFPSLIFNYLGQAALVLEGA
                     STEHNIFYMLCPSDFLLPLIILSTVATIIASQAIITGAFSMTRQAMQLGWLPRLRVTQ
                     TSSEGYGQIYIGVVNWFLMLATLGLTIGFGSSEKLAAAYGIAVSATMLCTTVLLFIAL
                     HKLWKWNIITSGLVAGLFMIVDASFFAANLTKFINGGYIPITLAIIIYSMMYIWHKGY
                     KTIAIKQKEKNITVDSFLDSIQKEGVVRVPKTAVFLTSKEQDIPPILVWHVKKNHVLQ
                     DKVIILNINNLSIPWCKPGDQLQIVETGAGIWHAVANYGFMEQPHIPKLLKKLEAQGY
                     DINIKDITYYIGHETIFVRNVRHTMSKYIKILFVFMHRNALPMSNYFHLPPESVFEIG
                     RQIEI"
     gene            2132..3898
                     /locus_tag="AC06IO_001920"
     CDS             2132..3898
                     /locus_tag="AC06IO_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875098.1"
                     /translation="MVEFAKPEQEPSADIGRIDLYIHKFGKYENIIKKWVGTLERNGK
                     NHPDEASKFQFGKIIGNILRKSLKKEAISPNNFKNLQKGQIWEEPISKYDREKNGIIQ
                     YTYRVYFNIPGVNHSDQEKINANKEQAQIHHFTTMTNDGLMVFSFEDKVEEIEPKLFL
                     ELAKNIEVESDAWAQLIDFTEKNFHTPEDLNSNPQVPQILGLILYATIFPEHRNEAFN
                     GLCPLLLKSENPNFNSITTKLLKDIIPDYEAKVMAFLHKNNNPMRYNENDISKQGALL
                     GLSREKVEVVKNEMRERKSLEEQNNNRSQAIELRKTLIGAVDEYLRWRNNESKETDYK
                     KSSGAFTWLRHYTDFGKNRANDLKNDLNKAQDLNTMLDILQKHFANNSRLHNHSLDSY
                     LLRAIYKDSKDSNKFNSIFNFKHLTIKNDTDANREWLREEMLEMAKKTNMNVTLENPT
                     VSRQESPTLPNKAKIRISIMKIPTNEREENILAVHTVLKNDELLRNQGGSVPTIIKEI
                     RDIVGKIDPSEEENIATAIIEIKGKIAGNSDKNYNENAHDIIKAFENPNCCDFRKIRA
                     ALTTNPAMDEIMNPVRVGMQLN"
     gene            4100..6838
                     /locus_tag="AC06IO_001921"
     CDS             4100..6838
                     /locus_tag="AC06IO_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HIO9875099.1"
                     /translation="MEEQQHISAPGWHEQSVEKTLITLNTTERGISEQESTKRLEYYG
                     FNRLPEPKRKSIFLRFLLQFHNILIYVLIGAAVVTSFLQHWVDTAVIIAVVIINALIG
                     FIQEGKAEKALDAIRKMLSPAATVLRDGERRHISGERLVPGDIIFLEAGDKVPADVRL
                     VRSHGLRVQEAILTGESIPVEKQIHPVAKDTELGDRTCMAFSGTLVTNGQGKGIVVAT
                     GSSTQIGHISGLLSKVESLTTPLVLQIGRFAKWLTLFILLIAALLLFYGYFIQNHPFA
                     ELFMVVVSLSVAAIPEGLPAVLSITLAIGVQTMAQRHTIVRRLPAIETLGSVSVICTD
                     KTGTLTLNEMIVSSVLTSNHLFILDGIGYEPQGTITLNEQVIDNNEYPLLKKLARAAI
                     LCNDAALHNQEGNWTVEGDPMEGALLAFAGKTGLAMQEENVLWVRTDVIPFDARHRFM
                     ATLNHDHLQHAMIFVKGAPEQILKMCQNQQINQGEVQPLNEPYWKRQMEQVAARGQRL
                     LAFAVKKTKPEHTVLEFADIKDGLTLLGMVGLIDPPRPEAIEAVAQCHTAGIQVKMIT
                     GDHASTALAIGRQIGLKNLDKVLTGIDLENMDDAILKNAVLETDIFARTSPEHKLRLV
                     MALQSHGMTVAMTGDGVNDAPALKRADAGIAMGKKGSEVAKEAAEFVLVDDNFASIVA
                     AVREGRTVYDNLKKVISWTLPTNAGESMTVILAILLGLSLPVSPIQILWINLITAVTL
                     GIALAFEPTEESTMHRPPRPRNEPLLTGSLVWHIILVSILFICGIFGLYFYALDRGYS
                     IELARTIALNTLVVLEIFHLFYIRNIYSTSLTWKAVQGTKVVWIAIIVVTVAQFAITY
                     FPPLQTVFVTESVPFGDGVLIVLTGATFFAVIEIEKQLRLRLRVISPPSAREKCSL"
     gene            7003..7566
                     /locus_tag="AC06IO_001922"
     CDS             7003..7566
                     /locus_tag="AC06IO_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875100.1"
                     /translation="MTIYFLFGKTGSGKSYIGELLRQLSIVHIDGDNHITQRMRDCLI
                     EDEQMTPEMIDEFVDVLIDVIKTQKTKTPNESFVISQALYLDKHRLKLLNAIPELKFV
                     MIDVTPVLRASRITNRFRNQESKVSLRYANKMDTFFEYPSHETIRLENNQGSDETLIE
                     QIREKLPALFNMDSKEELNSGIKLQFA"
     gene            complement(7681..8607)
                     /gene="leo"
                     /locus_tag="AC06IO_001923"
     CDS             complement(7681..8607)
                     /gene="leo"
                     /locus_tag="AC06IO_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease Leo"
                     /protein_id="HIO9875101.1"
                     /translation="MKNKIVLLAPVSLSFLFSFPTYAADYEFNGLSLSASLGILSGNA
                     HEYVYHPETDTKLSQLDWRIKNAPIIKGELNYDVLDWLSMNGRGWTTLVKNKAAMDDY
                     DWFNPYQETWTHWSHHEDTYLNYANELDMSLRAWLMQKQNYKLGLATGYQWNSFGWQA
                     TGGCYQYNGGLYAGCFPSDQPGIGYQQKFRTPYIGLAGKYFINNFEFSGLLKFSDWVS
                     ARDHDEHYARNLTFNERGNSSRYYAATIHSGYFLKPNTKIFVEASYNHYSNGRADTEI
                     IDNDTGERSYFGDSAGLSNKHYTVALGLQYLF"
     gene            complement(8735..10342)
                     /locus_tag="AC06IO_001924"
     CDS             complement(8735..10342)
                     /locus_tag="AC06IO_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIO9875102.1"
                     /translation="MNKNLSALSLLWISITSMVGSGWLFGSLYSAHFAGPAAIIAWPL
                     AGFLLLFVALSYAELGTMFPQSDSLACLPLYTHGRLTGIIMNVMTWFSLAILPVIETQ
                     GVIQYASNYIPDLVSHDALHYRNTPAGYMLSLVVLMSFVLFNYFGIGLFARINAAFTI
                     WKIIIPILTIASLVTMSYHPGNFFQHGGFMPYGWQGVMAAMSSGGVLFSLLGFRQVVI
                     MMGELEKPGQYIPLILGGSLVFVTLLYTGLQWSFIGSVSEQALEKGWVNLSFTGDAGP
                     FAALAALAGMLWLSILLYADAFISPYSTGLVYSTTAAHMLSSMGSVVPAPVILTKQNR
                     YQVPWVSLGVNFLLAATMSLVLHGWQEMSAFLVAILMISYSIGPVALVCLRQQLPHYK
                     RPFRLQGSNLIGFLGFYVCTVGVYWAGFHSVRKLLVITILGVVCSFLYCLMKNTHREI
                     DSKQALWFIFYLFGLGIFSFLGNYGGKHILPQYWDLLYLLVFSLLVFGFALLSRKPSA
                     HTQNMVAQNTHTNITAPAFSIERYDIA"
     gene            10673..12838
                     /gene="katG"
                     /locus_tag="AC06IO_001925"
     CDS             10673..12838
                     /gene="katG"
                     /locus_tag="AC06IO_001925"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HIO9875103.1"
                     /translation="MDGKVGNTATGCPVMHGGMTSAETSNTAWWPNALNLDILHQHDT
                     KTNPMGKDFNYREEVKKLDFEALKKDLHALMTDSQPWWPADWGHYGGLMIRMSWHAAG
                     SYRVADGRGGAGTGNQRFAPLNSWPDNVNLDKARRLLWPIKKKYGNKISWADLIVLAG
                     TIAYESMGLKTFGFGFGREDIWHPEKDVYWGSEQEWLGAKRYDGKSRESLENPLAAVQ
                     MGLIYVNPEGVNGQPDPLRTAQDIRVTFGRMAMNDEETVALTAGGHTVGKCHGNGNAK
                     FLGPDPEAADVEDQGLGWINKTTRGIGCNTVSSGIEGAWTTHPTQWDNGYFYLLLNYD
                     WELKKSPAGAWQWEPIHIKEEDKPVDVEDPAIRHNPIMTDADMAIKMDPVYRKIAERF
                     YQDPDYFAEVFARAWFKLTHRDMGPKTRYIGPDVPKEDLIWQDPVPAGNRAYDIAAAK
                     AKIAASNLTIGEMVSTAWDSARTFRGSDKRGGANGARIRLKPQKDWEGNEPQRLTKVL
                     RILEGIAADTGASVADVIVLAGNVGIEKAAKAAGFDIIVPFAPGRGDATDDMTDAESF
                     DVLEPLHDGYRNWLKKAYDVRPEELMLDRTQLMGLTAHEMTVLVGGLRVLGTNHNNTQ
                     HGVFTDRVGALTNDFFVNLTDMANVWIPSKDNLYEIRDRKTGNIKWTATRVDLVFGSN
                     SILRSYAEVYAQDDNKGKFIQDFVAAWTKVMNADRFDLA"
     gene            complement(13149..>13714)
                     /locus_tag="AC06IO_001926"
     CDS             complement(13149..>13714)
                     /locus_tag="AC06IO_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875104.1"
                     /translation="YTYMYYMEDYKMHILPQVNALLAKFHYPAIDKVNFCQAKQFSQE
                     GCGIAMGLNMQKILEENYPQVICYSNTPRQGIPSRTEIRCLSSNVSIEEDALMRVELA
                     FKTQARASIIAEGALDAKMVEKKSLVCSEILSDSRSLNVKAKEAFGFFQSAQPGISSE
                     MHSVMAQDKGNDEASSSDLNAGACRLW"
BASE COUNT         4329 a         2810 c         2808 g         3767 t
ORIGIN      
        1 agtgccactt attggaggat agcccctcct tttgagtaaa gccaaactta atgagagagc
       61 ccactcaatg ccctcaacaa gaaacattga aacgcataac gactccaatc ccacattgcg
      121 agcactgagt ctatctgcct tgggcatagt ttacggagat attggaacaa gccctcttta
      181 tacttttaaa acggttatat tgctggcggg aggagggact ccagacgtaa ctaccattat
      241 gggctcagta tcattgatta tatggactct gataatcatt gcttcggtta aatacatctc
      301 ctttgcttta cggattgaca acgatgggga gggaggtatt ctcgcgttga tgtctctttt
      361 aagtttaaaa ctaaaacaaa agcctttcat tattgcggtt ggacttatgg gagctgctct
      421 tatctatgga gatggcacaa ttaccccagc catatcggta ctctctgctg tagagggact
      481 tgaaatcctt tccccatcac ttaagtatta tgttttgcct attgcgataa caattctaat
      541 aacacttttt gctattcaat ctaaaggtac cgcaactatt ggaaaggcct ttggcccagt
      601 gatggcattt tggtttttaa ccattggtat tttaggggca agaggggtta tccaacaccc
      661 ttttgtctta gcggcgataa accctgttta tggcttaaat tttttatttt caaatggcgc
      721 aacaggattt tttattttat gtggggtatt tctttgtgtc actggtgcag aagccttata
      781 cgcagacctg ggacattttg gtaccgcccc aattcgctgc gcctggtttg gtctggcttt
      841 tccaagcctt atctttaact atttgggaca agctgctttg gttttggaag gagcatccac
      901 agagcataat attttttaca tgctgtgccc aagtgacttt ttattaccgt taataattct
      961 gtctaccgtc gcaacaatta tcgccagcca agccattatt accggagcat tttccatgac
     1021 aagacaagca atgcaactgg gctggctacc caggttacga gtcacgcaaa cgtcttcaga
     1081 gggttatggg caaatttata taggagttgt taactggttt ttaatgctgg ctacccttgg
     1141 gcttactatt ggatttggca gttctgaaaa attggctgct gcctatggca ttgctgtttc
     1201 agccactatg ctttgcacca ctgtgctttt atttattgct ctgcataagc tatggaaatg
     1261 gaatattatc acatcagggc ttgttgctgg attattcatg attgtcgatg cctctttttt
     1321 tgcggccaat ctcacgaaat ttatcaatgg cggatatata ccaatcacat tggcaatcat
     1381 aatctattcc atgatgtata tttggcataa aggctataaa accattgcga tcaagcaaaa
     1441 agaaaaaaat ataacagtcg attcctttct ggacagtatt caaaaagagg gggtagtccg
     1501 tgtgcctaaa actgctgttt ttcttacatc caaagagcag gatattcctc caatcctggt
     1561 ttggcatgta aagaaaaatc atgtgttgca ggataaggtt attattttaa atattaacaa
     1621 cttgtccatt ccctggtgta aacctggtga tcaattacaa attgtagaaa caggagcagg
     1681 catctggcat gccgtagcca attatggctt tatggaacaa cctcatattc caaaattatt
     1741 aaaaaaatta gaggctcaag gctatgacat caatatcaaa gacataacct attatatcgg
     1801 tcatgaaaca atatttgtcc gcaatgtccg tcatactatg tcaaaataca ttaaaattct
     1861 cttcgttttt atgcatcgca atgcccttcc catgagtaat tactttcatc ttccgccaga
     1921 atcagtattt gaaataggac gacagataga aatttagtga aaatgatttt ataagcaaga
     1981 cttaagctgt ccaccaaatc attagtacag attaacaatc aaaattcaaa cctgatgcgg
     2041 aagttatcac agacatatcg ggaaccttga aaaaaaaagc actcaaagat caactataat
     2101 gcttaataag cgagtaatta agaagatgaa aatggtcgaa tttgccaaac ccgaacagga
     2161 accctcagca gacattggac gaatagatct ttacattcac aaatttggga aatacgaaaa
     2221 tatcattaaa aaatgggttg gtactctcga gcggaacggt aaaaatcacc cagatgaagc
     2281 ttctaaattt caatttggga aaattatagg caatattctg cgtaaaagct taaagaaaga
     2341 agcaatatcc ccaaataatt tcaaaaatct tcagaaaggt cagatttggg aagagcctat
     2401 ttctaaatat gacagagaaa aaaatgggat aattcaatat acctatcgag tttactttaa
     2461 catcccagga gttaatcatt cagatcagga aaaaataaat gccaataagg agcaggcaca
     2521 aatacatcat ttcacaacta tgactaatga tggtcttatg gttttttcct ttgaagataa
     2581 agtagaggaa atagagccta aattatttct ggaactggca aaaaacatag aggtagaatc
     2641 agatgcatgg gcacaattaa ttgacttcac agaaaaaaat ttccacaccc cagaagattt
     2701 gaattccaat ccacaagtcc ctcaaatatt aggtctgatt ttatatgcaa ctatctttcc
     2761 cgaacacagg aacgaggctt ttaatggtct gtgcccattg ctcttaaaat cagagaaccc
     2821 taactttaac tcaattacaa caaaactgct taaagatatt attcctgatt atgaagccaa
     2881 agtgatggca ttcctgcata aaaataacaa tccaatgcgt tataatgaaa acgacatcag
     2941 caaacaagga gcccttttag gtctttcaag ggaaaaagtt gaagtagtaa aaaacgaaat
     3001 gagagaacgt aaatcattgg aagagcaaaa caacaatcga tcacaagcca ttgaacttag
     3061 aaaaactctc attggcgcag tagatgaata ccttagatgg cgcaataatg aaagcaaaga
     3121 aacggattat aaaaaaagct caggtgcttt cacctggtta cgccattata ctgatttcgg
     3181 caaaaatcga gccaatgatt taaagaacga tctgaataaa gcgcaagatt taaacaccat
     3241 gctggatatt ttacaaaaac atttcgcaaa caattcccgg ttacataatc attctcttga
     3301 cagctacctt ttaagagcca tctacaaaga ctctaaagat tctaataaat tcaattcaat
     3361 atttaatttt aaacatctta caattaaaaa tgatacagat gctaaccgtg aatggctaag
     3421 agaagaaatg ttggaaatgg caaaaaaaac aaacatgaat gttacattgg aaaatccgac
     3481 agttagtagg caagaaagcc ctactctacc caataaagct aaaatccgta tcagtataat
     3541 gaaaatacct actaatgaac gagaggaaaa tattttggcg gtccacacgg tactaaagaa
     3601 tgatgaattg ctgagaaatc aaggtggctc agttccaact atcataaagg aaatacggga
     3661 cattgtgggg aaaatcgatc cgtcagaaga agaaaatata gccactgcca ttattgaaat
     3721 caaaggaaaa atagctggca acagtgacaa gaattataat gaaaatgcgc atgacatcat
     3781 caaagcattt gagaatccaa attgttgtga ttttagaaaa atccgcgctg ccttaacgac
     3841 caacccagca atggacgaaa taatgaatcc tgtcagagta ggaatgcagc taaactagtt
     3901 caaattattt tgtcaaagat tatttttcta cttcgcagct atgctgctcc caagacggaa
     3961 ttgttcggat ttaccaaaag actgattaac actgaaacaa ccatgattcg aaaatgcgca
     4021 aaatactttt aaatattata atttcagcaa tatcagttga gcgttggacc gccaacaaga
     4081 agaaaaggac aaaaagataa tggaggaaca acaacatatt tcagctcccg gttggcatga
     4141 gcaatcagta gaaaaaacac ttattacgct aaacactaca gagcgaggga taagtgagca
     4201 ggaatccaca aagcgattag aatattatgg tttcaatcgc ttgccagaac caaaaaggaa
     4261 aagtattttt ttacgcttct tgctccagtt ccacaacata ctcatctacg tattaatagg
     4321 tgcggctgtg gtaacctcct tcctgcaaca ctgggtagat accgctgtta taatagctgt
     4381 tgttatcatc aatgccctga taggttttat ccaggaagga aaagcggaaa aagcgctgga
     4441 tgcgattcga aaaatgctgt ctcccgctgc cacagtattg cgcgacggag agagacggca
     4501 tattagcggt gagcggcttg tgccaggcga tattattttt ctggaggcag gagataaagt
     4561 tcctgctgac gtacgcttag taagatctca tggtttgaga gttcaggaag ctatattaac
     4621 aggcgaatcc attcctgttg aaaaacaaat tcatcctgtc gcaaaagata cagaattagg
     4681 cgacaggact tgtatggcct ttagtggaac attggtaacc aatggccaag gcaaagggat
     4741 tgttgtagca acaggatcat ccacccaaat aggacatatt agtggcttat tatccaaagt
     4801 agagtcatta accacaccat tagtcctaca aattgggcga tttgcaaaat ggctcacttt
     4861 atttattctg ttgattgccg cactgcttct tttttatggt tattttatcc aaaatcatcc
     4921 gtttgccgaa ttgtttatgg tcgtggtcag tttatcagtt gctgctatcc ctgaaggtct
     4981 tcccgccgtt ctttcaatca ctctggccat tggagtacag acgatggcgc aacgacatac
     5041 cattgtgcgc cgattacccg ccatcgaaac cttaggctcc gtatcggtta tctgtactga
     5101 taaaaccggt accctgactc tgaacgagat gattgtctct tctgtcctca ccagtaatca
     5161 tctatttatt ttggatggca tcggttatga gcctcaagga acaattacct taaacgaaca
     5221 agtcattgat aacaatgaat atcctttgct taagaaactc gcgcgtgccg ccatactttg
     5281 taacgatgct gcacttcaca accaggaagg aaactggact gtagaaggcg accccatgga
     5341 gggcgcctta ctggcatttg ccggtaaaac aggactggct atgcaagaag aaaacgtctt
     5401 gtgggtacgt accgatgtga ttcctttcga tgccagacat cggttcatgg ccaccttaaa
     5461 ccacgatcat ttgcagcatg caatgatctt tgttaaaggt gcccctgagc aaattttgaa
     5521 gatgtgccaa aatcaacaaa tcaatcaggg agaagtacag cctttaaatg agccttattg
     5581 gaagcgacaa atggagcagg ttgccgcaag aggacagaga ttactggcct ttgcggttaa
     5641 aaaaacaaag ccagaacaca ccgttttgga atttgccgat attaaggatg gtttgacatt
     5701 acttggaatg gttgggttaa ttgatcctcc aagacctgaa gccattgaag ctgtagcaca
     5761 atgtcataca gcaggcattc aggttaaaat gattacagga gatcatgcca gcacagcatt
     5821 agccattggt cgccaaatag gacttaaaaa cctggataaa gtattaacag gcattgattt
     5881 ggagaatatg gatgatgcga tacttaagaa tgctgtgttg gagacagaca tttttgcccg
     5941 aaccagtcct gagcataaac taagactggt gatggcattg caatcgcacg ggatgactgt
     6001 ggccatgacc ggtgatgggg tgaatgacgc tcctgcgtta aaacgagcag acgcaggaat
     6061 tgccatgggc aaaaaaggca gcgaagtagc caaggaagct gcggaatttg tacttgtgga
     6121 tgataatttt gcttctattg tcgcagccgt tcgtgaaggt cgtactgttt atgacaacct
     6181 gaaaaaagtc atcagttgga ctttacctac caatgcgggt gagtcaatga ccgttatttt
     6241 agctatactt ttgggactca gtttgccagt ttcaccaatt cagattttat ggattaactt
     6301 aattaccgca gtcactttag ggattgcgct ggcttttgaa ccaacggaag aaagcaccat
     6361 gcatcgtcca ccacgtcctc gaaatgaacc tctgctaacc ggcagtttag tatggcatat
     6421 tattctggtt tccattttat tcatttgcgg gatttttgga ctctatttct atgcactcga
     6481 tcgcggttac tcgattgagc tggctcgcac aatcgctttg aatactctag tggtacttga
     6541 aatttttcat ctgttttata ttcgtaatat ttatagtacc tcactcactt ggaaagcagt
     6601 tcaggggaca aaagtagttt ggatagcaat catagtggtt actgtagcac agtttgcaat
     6661 cacctatttc ccgcctttgc aaacagtatt tgttaccgaa agtgttccat ttggggatgg
     6721 agtattaatc gttctaactg gagctacatt ttttgctgtg attgaaattg aaaagcagct
     6781 tcgtttgaga ttaagggtta taagccctcc aagtgcaaga gaaaaatgct cattataatg
     6841 ataaactcct gtgatggatt ggttataaat ccatcgtcta attctgacaa tcaagtcctc
     6901 tctggttagg caatgactaa gaaagtacac atttccatga ttttaaggta tggttattca
     6961 ctcacctaat gtgaaaaaca tacattacaa gggttgtgaa caatgaccat ctattttttg
     7021 tttggcaaaa ctggctcggg taaaagttat attggagagc tgctcagaca gctaagcatc
     7081 gtccatattg atggtgataa ccacatcacc caaagaatgc gtgactgtct aatcgaagat
     7141 gaacaaatga ccccagagat gatcgatgaa tttgtcgatg tcttgattga tgttatcaaa
     7201 acacaaaaaa ctaaaacacc gaacgagagt tttgttattt cacaagcctt gtatctcgac
     7261 aagcaccgtc tcaagttatt aaacgccata cccgagctca aatttgtaat gattgatgtc
     7321 actcccgtac tgagggcaag ccgcatcacc aatcgatttc gcaaccagga aagcaaagta
     7381 agcctacgct atgctaacaa aatggataca ttttttgagt atccttcaca tgaaacaatc
     7441 cggcttgaaa acaatcaagg ctcagatgaa acgcttatag agcaaatccg tgaaaaattg
     7501 ccagcactgt ttaatatgga ttcaaaagaa gaattaaact caggcataaa gcttcagttt
     7561 gcataatatt cagatccata tccagatatg gttgaaataa agaacttatg tagcaactgt
     7621 cttgaagtta ggacttaaga cagttgccac ttgccagttg ggattagaaa atttgacctt
     7681 ttaaaacaga tattgtaaac caagagctac agtatagtgt ttattggata gccctgctga
     7741 atcaccgaaa taactgcgct cacccgtgtc attgtcaatg atttcagtat ccgctctccc
     7801 attagaataa tgattgtaag aagcctcaac aaaaattttc gtattaggtt tcaaaaagta
     7861 gccagaatga atcgttgccg cataatatct ggagctattt ccccgctcat tgaatgttaa
     7921 gttgcgggca taatgctcat catgatctcg agctgaaacc caatcactaa attttaaaag
     7981 accactaaac tcaaaattat taataaaata cttccctgcc agaccaatat aaggagttct
     8041 aaatttttgt tggtatccaa ttccaggttg atcactggga aaacaaccgg catagagccc
     8101 tccgttatat tgataacagc ctccagtagc ttgccagcca aaggaattcc attgataacc
     8161 agtggcaaga cctaacttgt aattttgttt ttgcatcaac caggccctta agctcatatc
     8221 taactcattc gcataattga gatacgtatc ctcatgatga gaccagtgag tccaggtttc
     8281 ttgataagga ttgaaccaat cataatcatc catagcagcc ttatttttta ccaaggttgt
     8341 ccaacctcga ccattcatgc taagccaatc caggacatca taatttaatt ccccttttat
     8401 tatcggagca ttttttatgc gccaatccag ctggcttaac ttggtgtctg tctctggatg
     8461 gtaaacatat tcatgggcgt tacctgacag aatgcctaga gaagcactta atgacaaacc
     8521 attaaattcg taatccgcag cataagtagg aaatgagaaa agaaaggata aagagactgg
     8581 agccaacaaa actattttat ttttcatatt ttttcctagt taaggctggc aatccttgca
     8641 gcaaagtgac tttaaaaaac caatcgcatt attgtgatcc ataatgatct tgtcaagatc
     8701 aacattgaat aatattgatc actaaggact ctaatcaagc aatatcatat cgctcaattg
     8761 agaaagcagg agcagttata ttagtgtgtg tattttgggc aaccatgttt tgagtgtggg
     8821 cactgggctt tctggacaat agggcaaaac caaatacaag caaactgaaa actaataaat
     8881 acaacaaatc ccaatactgg ggaaggatat gcttaccacc ataattacca agaaatgaga
     8941 aaataccaag tccaaaaaga tagaatataa accatagtgc ttgtttgcta tcaatctccc
     9001 gatgtgtatt tttcataaga caatagagga aagaacacac tactcccaga atagtgatca
     9061 ccaataactt tcgtacgcta tggaaacctg cccaataaac accgactgta caaacataaa
     9121 atccgagaaa gccaataagg ttactcccct gcaaacgaaa aggacgctta tagtgcggta
     9181 attgctgacg taagcaaacc agtgctacag gccctatgga atagctaatc attaatattg
     9241 ccacaagaaa agccgacatt tcttgccatc cgtgtaacac gagcgacatg gtggcagcga
     9301 gtaagaaatt tacgcctaaa ctaacccagg gaacttgata cctgttttgt ttggtaagaa
     9361 ttacaggtgc aggaacaact gagcccatac tgcttagcat gtgagctgct gtggttgaat
     9421 aaacaagacc agtggaataa ggtgatataa aagcgtcggc gtacaataaa atgctcaacc
     9481 aaagcatgcc agccagtgca gcgagagcag caaatggtcc tgcatcacca gtaaaagata
     9541 aattcaccca acctttttcc agcgcttgtt cgctaacact cccaataaag gaccattgta
     9601 aaccagtgta aagtaacgtc acgaacacta gtgaaccacc caaaatcaga ggtatgtatt
     9661 gtcctggttt ctccaactca cccatcatga taaccacttg tcgaaaccct aaaagtgaaa
     9721 aaagcacacc tccgcttgac attgctgcca taacgccctg ccagccataa ggcataaaac
     9781 caccgtgctg aaaaaaatta cccgggtgat aactcatggt taccaagctt gcgatagtaa
     9841 gaatagggat tattatcttc cagatagtaa atgcggcatt aatgcgggca aacaaaccaa
     9901 taccaaaata attaaacaaa acaaaactca ttaatacaac cagggacagc atataacctg
     9961 ctggcgtatt tcgatagtgc aaagcgtcat gggataccag atctggaatg taattactgg
    10021 catattgaat gacaccttgc gtttcaatca ctggcaaaat ggccagggaa aaccaggtca
    10081 tgacattcat gataatgcca gtcaaacgtc catgggtata taaaggcaag caagccaggc
    10141 tgtcagactg tggaaacatc gtacctaact cagcgtaaga caaggcaaca aacagaagta
    10201 aaaaacctgc cagtggccaa gcgataatgg cagcaggacc agcaaaatga gcagaatata
    10261 aagaaccaaa taaccagcct gagcctacca tgctggtaat tgaaatccaa agtaaactca
    10321 aagcacttaa atttttattc atctgatttt cacatgtcct tatgattttg atgcaggcaa
    10381 acaattcacc agtcagcaga gcgaaatcat tatatttttg cattgtaaac agaaatatca
    10441 tgactaccaa ccctataaga gtcaagttta accaggtagg ttacgaatac cttccagcat
    10501 caggaaaaat gaaaaacaag aactagcagc agtgattttc tttgctacgg attcggcatt
    10561 tttcactctg taatctctcg cttgacagta tctctgcttc gattgcttag ctgccctccc
    10621 ttaagctata gttaggtctc tagtgaatta tgatctgcga ccaggagtgt ctatggatgg
    10681 aaaagttggg aatacagcaa ccgggtgtcc ggtgatgcat ggcgggatga catctgcaga
    10741 aacatccaac acagcatggt ggcccaatgc cttgaacctg gatatattgc atcagcatga
    10801 caccaagaca aatccaatgg gaaaagattt caactatcgt gaagaagtca agaaactgga
    10861 ttttgaggcg ctcaaaaagg atttgcacgc tttgatgact gacagccagc catggtggcc
    10921 tgctgattgg gggcactacg gtggtttaat gattcgtatg tcctggcatg ctgcaggttc
    10981 ttatcgtgtt gcggatggcc gcggcggtgc tggtacaggc aatcaacgct ttgctccatt
    11041 gaattcctgg cctgacaatg tgaacctgga taaagcacgt cgcttattat ggcccatcaa
    11101 gaaaaaatat ggcaacaaga ttagttgggc agacttgatt gtcctggctg gcaccatcgc
    11161 ttatgagtca atgggactaa aaactttcgg ttttggtttt ggccgcgaag acatttggca
    11221 tcccgaaaaa gatgtttatt ggggttcaga acaagaatgg ttaggcgcca agcgttacga
    11281 tggcaaaagc cgtgaatctc tggaaaaccc gctggcagca gtacaaatgg gattaattta
    11341 tgtaaaccct gaaggagtga atggtcaacc tgacccgctg cgcactgccc aggatattcg
    11401 tgtaaccttc ggacgcatgg ctatgaacga tgaggaaacc gttgctctga ctgccggagg
    11461 acatactgtt ggcaaatgtc atggtaatgg caatgcaaaa tttttgggac ccgatcccga
    11521 agccgctgat gttgaggacc aaggccttgg ctggattaac aaaaccacca gaggcatcgg
    11581 ctgtaatacc gtttcaagcg gtattgaagg cgcatggacg actcacccta cccagtggga
    11641 caatggctac ttctatttat tgctgaatta cgattgggaa ctgaaaaaaa gccccgccgg
    11701 tgcctggcaa tgggagccca tccatattaa ggaagaagat aaaccagtag atgttgaaga
    11761 ccctgccatc cggcacaatc cgatcatgac tgatgctgac atggcaatca aaatggatcc
    11821 tgtttatcgt aaaatcgccg agcgctttta ccaagatccc gactattttg cagaagtttt
    11881 tgcacgagcc tggtttaaac tgactcatcg cgatatgggg ccgaagacgc gctacattgg
    11941 ccctgatgtt cctaaagaag atttgatttg gcaagatccg gtgcctgctg gtaacagggc
    12001 atatgatatt gctgccgcca aagcaaaaat tgcggccagc aatctgacca tcggtgaaat
    12061 ggtttctact gcatgggaca gcgcgcgcac ttttcgcggc tcagataagc gcggtggtgc
    12121 gaacggagca cgtatacgac taaagccaca aaaagactgg gaaggcaatg agccacaacg
    12181 cctgactaaa gtcttgcgaa ttcttgaagg catcgctgct gatacaggcg caagcgtggc
    12241 agatgtgatt gtacttgccg gaaatgtcgg tatcgagaag gctgccaagg cagctggctt
    12301 tgacattatc gttccctttg ctcctggccg tggggatgca accgatgaca tgacggatgc
    12361 agaatccttt gacgttctgg aaccacttca tgatggctat cgcaactggc ttaagaaggc
    12421 atatgatgtc cgtcctgaag agctcatgct agatcgcact cagctcatgg gactgacagc
    12481 ccatgagatg acagtccttg ttggaggtct gcgcgtgttg ggtactaatc acaacaatac
    12541 ccaacatggt gtattcactg atcgcgttgg tgccctgact aatgactttt ttgtcaactt
    12601 gaccgatatg gccaatgtat ggataccaag caaagacaat ttgtacgaaa ttcgagaccg
    12661 taaaacgggt aacatcaagt ggacagcgac tcgtgtagat ctggtttttg gctctaattc
    12721 gattctgcgc tcctatgctg aagtctatgc ccaggatgat aacaagggaa aattcataca
    12781 ggactttgtt gcagcatgga caaaggttat gaatgccgat cgctttgatt tggcataatc
    12841 ctttgcaagt tgtcatacca tagataatca tagtagaaat agtttcgatt atgtttagct
    12901 ttgattgcct tttatcgcat caaaactaaa catagcgtac taccataaaa tgttgtttca
    12961 aaagcgaaca tttacgatgg tttgcattag gcctgatttg cctaacttgt ctcttttgtt
    13021 ttatttctgc gttgctaacg tttctgtaag tttcatctac tgcatgtaaa ctccactctt
    13081 caaaacgttt gcgccttgcc ctaaaacaaa ataatcagct catgcaagag gtctatttat
    13141 attatgtctc accacaaacg acatgctcca gcatttagat cactggaaga tgcttcatca
    13201 ttgcctttgt cttgtgccat aaccgaatgc atttcagacg atattccagg ttgtgctgat
    13261 tgaaagaaac caaatgcttc tttagccttt acatttaaac tgcgtgaatc acttaaaatt
    13321 tcactacaaa ccaagctctt tttttctacc atctttgcat caagagcgcc ctctgcaata
    13381 attgatgcgc gtgcttgggt tttaaatgca agctctactc gcattaaagc atcctcttca
    13441 atactcacat tacttgataa acatcgtatt tctgtacggc ttggaattcc ttgtctcggt
    13501 gtattagaat aacaaataac ttgaggatag ttttcttcca gaattttttg catattaagg
    13561 cccattgcaa taccacaacc ctcttggcta aattgttttg cttgacaaaa attgacttta
    13621 tcaatggctg gatagtggaa tttagccaat aacgcattga cctgtgggag gatatgcatt
    13681 ttgtaatctt ccatgtaata catataagtg tagt
//
LOCUS       DBNCWK010000077        13615 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.077, whole genome shotgun sequence.
ACCESSION   DBNCWK010000077 DBNCWK010000000
VERSION     DBNCWK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13615
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.077"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            69..1160
                     /locus_tag="AC06IO_001927"
     CDS             69..1160
                     /locus_tag="AC06IO_001927"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIO9875105.1"
                     /translation="MEMMRQQRNLLLIPFILCFILARPVFSLNLDALLPDERNTVEVF
                     QKASSKVVYVHRLANATVQRRYSLQKTHIPDGAGSGIIWDNKGHVVTNFHVINGADDI
                     AITLGNMTVPAKVIGSEPRKDIAVLEIKSPKALNYLKSFQPFEIVSLNDLIVGQKAIA
                     IGNPFGLDHSLSKGVISALGRKVPGIGGVTIYDMIQTDTPINPGNSGGPLLNSAGQLI
                     GMNTMIYSRSGSSAGIGFAVPAEDIQKIASQIINHGRVVLSGIGIQRVEPHLAERLGV
                     KKGILIADVVPGTPADKLKLRGTHRDQWGRIVLGDVIVGVNAHPVPNYDALYNLLTEI
                     KVGEQITVSIIRHGKKMDVKMKTIDIAAL"
     gene            1280..1825
                     /locus_tag="AC06IO_001928"
     CDS             1280..1825
                     /locus_tag="AC06IO_001928"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIO9875106.1"
                     /translation="MLLNRADSLLVLIDVQEKLTPAILNLDLFLARCEWLLKLARKLD
                     VPVLASEQYPKGLGRTVSPLSSYINQEECIEKIHFSCMQEPQYINLLKGYNKNQLILI
                     GIEAHVCVLQTAIEMKGYGFEVFVVVDGVSSRSEFDLKYGLKRMKQNGIHLITAEMVL
                     FEWLKQAGTPEFKAISKEFLQ"
     gene            2034..2777
                     /locus_tag="AC06IO_001929"
     CDS             2034..2777
                     /locus_tag="AC06IO_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9875107.1"
                     /translation="MNFDNQIALVTGGVSGMGKACVQYLQQHGMKVVVWDKQEGTSNE
                     AELYVACDVTSDESVEKAMRQTISQVGVPRVCINCAGIAPAKRMVGKEGPMPLESFRQ
                     VIDVNLIGTFNVMRIAAHAMSGLELDNKSQERGVIINTASIAAFEGQIGQSAYSASKG
                     GIVSMTLPAARELAQFAIRVNTIAPGLIATPLLLNMPQEVQDSLVATVTFPKRLGRPE
                     EFASLVGHIIENQMINGEVIRLDAALRMR"
     gene            complement(2840..3337)
                     /locus_tag="AC06IO_001930"
     CDS             complement(2840..3337)
                     /locus_tag="AC06IO_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella synthesis protein FlgN"
                     /protein_id="HIO9875108.1"
                     /translation="MNNNNKAKTLITQLEQEISWVEELNTLLSEEKEILSTSQFNQLE
                     TLAEKKQELSTKLEASAKQRVDLIKESNPGDKPHGLLLSDFLKNCATEDGIQINHLNN
                     TLAEKLNLCRELNTINGQVIANNMYTRQQIVNALSGNKSNAVSVYTSNGNLETPSESS
                     HHEEA"
     gene            complement(3354..3674)
                     /gene="flgM"
                     /locus_tag="AC06IO_001931"
     CDS             complement(3354..3674)
                     /gene="flgM"
                     /locus_tag="AC06IO_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HIO9875109.1"
                     /translation="MINTIEDIHMVNQINDSANLRHIDMDNRINANHKEVKSPVLENN
                     STDSVNLSSTSKQLEALKASLKDLPEINEARVLYFKAEIQSGQYEIDSSKIAHGMLNS
                     VEMV"
     gene            complement(3765..4466)
                     /gene="flgA"
                     /locus_tag="AC06IO_001932"
     CDS             complement(3765..4466)
                     /gene="flgA"
                     /locus_tag="AC06IO_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HIO9875110.1"
                     /translation="MKKCILSFFLFFASTSLFSESSQSLELLKNKIEQYALNELSNYT
                     EGKIQVTADNIDPRLNLKVCDDNKLEVFNPYQTPMLNTSTMGIKCLEENNHWTLYIPV
                     KITIFKSVLVAKRALLKGTKISNSDIYQTELDVQKLKQGYFTDSKELIGLVCKHDITP
                     NSPLNPFNIELAKLVHKGEQVSIIAAHDNLTVSMDGIAMDEGALGDSVKVKNLSSKRI
                     IEAQVTGKKTVKVIF"
     gene            4553..4960
                     /locus_tag="AC06IO_001933"
     CDS             4553..4960
                     /locus_tag="AC06IO_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIO9875111.1"
                     /translation="MRPCYFVCILALFMFAMPGYSENHHPQEFLKSIAGTKDEGEKIF
                     NHFCVNCHGTKPMISLGAPEIGNERAWGARLKQGMEVLFKHTDEGFNAMPPRGGCFEC
                     TDKQLILAIIAMVPKQAQKGLLNNLLDHKEYKQ"
     gene            5037..5594
                     /locus_tag="AC06IO_001934"
     CDS             5037..5594
                     /locus_tag="AC06IO_001934"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIO9875112.1"
                     /translation="MKKQILLVPVCVLAASCASMDRSTAIVDDAGYTHYTMSMESDRK
                     GSNYFPEKRQATGKKVFIFDPKATAWAAYDAQGNRVKTGSASGGKDFCEDVGRPCRTV
                     TGSFKVYSKKGEECTSSIYPIETGGGAKMPYCMHFHGGYSIHAAYEVPNYNASHGCIR
                     VLPSAAKWLNQNFIDVGTSVIVKPY"
     gene            5863..6633
                     /locus_tag="AC06IO_001935"
     CDS             5863..6633
                     /locus_tag="AC06IO_001935"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIO9875113.1"
                     /translation="MILCIDVGNSHIYGGVFDGDEIKLRFRHTSKVSTSDELGIFLKS
                     VLRENNCSPETIRKIAICSVVPQVDYSLRSACVKYFSIDPFLLQAGVKTGLNIKYRNP
                     VEVGADRIANAIAATHSFPNQNIIVIDFGTATTFCAISHKKAYLGGAILPGLRLSADA
                     LSKNTAKLPSVEIIKTESVVGRSTIESIQSGVYYGVLGACKELIQRIHHEAFNGDKIL
                     ILATGGFASLFDKQGLYDHLVPDLVLQGIRLAAMMNTA"
     gene            7246..7704
                     /gene="mraZ"
                     /locus_tag="AC06IO_001936"
     CDS             7246..7704
                     /gene="mraZ"
                     /locus_tag="AC06IO_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIO9875114.1"
                     /translation="MFRGINAITIDTKGRLAIPTRYRSALGAEDKIPLVVTIDTEETC
                     LLLYTAAQWQIIEDNLQKLPSFNAAARRIQRLLIGHATDVEVDANGRVLLPTVLRNYA
                     KLEKDVVMIGQGNKFEVWNKELWESKREQWLAEEASMTDGLPEEMKTFSL"
     gene            7716..8642
                     /gene="rsmH"
                     /locus_tag="AC06IO_001937"
     CDS             7716..8642
                     /gene="rsmH"
                     /locus_tag="AC06IO_001937"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIO9875115.1"
                     /translation="MAKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSD
                     KGRLFAIDKDLDAVQYAKDYFGLDKRFQIFHGSFAQIKEFASQAGVIGAVDGILLDLG
                     VSSPQLDNPERGFSFMLQGPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFA
                     GRIAKAIVDARKLKPITTTLELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDL
                     SNCLEQCLDVLGPRGRLAVISFHSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNF
                     KRVGKAVKPQSSEIKENVRSRSAVLRIGEKLA"
     gene            8639..8977
                     /gene="ftsL"
                     /locus_tag="AC06IO_001938"
     CDS             8639..8977
                     /gene="ftsL"
                     /locus_tag="AC06IO_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIO9875116.1"
                     /translation="MNAAAKVINQGNLFNGQLADMQMSKSLYMLIVLLAAVLVSAFAV
                     IYSTNSYRLTLSQVQQEEQQTHFLQLQWGQLLLEQASLATPARVEELAREKLGMTLPT
                     SANTYLLHPQ"
     gene            9359..11020
                     /locus_tag="AC06IO_001939"
     CDS             9359..11020
                     /locus_tag="AC06IO_001939"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIO9875117.1"
                     /translation="MKKTHHFARLVTVAVFFSLILAVLIWRMVDLTVLHRQFLQGQGD
                     ARSIRVIDIPAYRGMITDRNGTPLAVSTPVESVWVNPKEFSPDEEQFMQLAKYLNLAP
                     KELSRKIVRAENREFVYLQRQLPPPLSKKIEALKIPGVNFQKEFKRYYPDSDSISPLI
                     GFTNVDDQGLEGIELAYQDWLMGVVGKKRVIKDRLGRVVEDLGVLKEPRPGHELTLSI
                     DRRLQYLAYSELGKTVEEFSAKSGSVVVIDAENGEILAVANVPSYNPNARGHYDKETY
                     RNRAFTDTFEPGSVIKSFSIASALETGLFTPDTIIDTNPGWMTVHGRTVRDIHNYGVL
                     DVTGILQHSSNVGVTKMVLSTPAEQLIGFLQKCGFGQRSELAYPGESEGGIVKAKDAN
                     PFVLATLGFGYGLSVTALQLAKANLIFANKGRLIPVTLIHNDTPSNGIQVMQPKTAEN
                     VLLMMEAVMNKGTGKGARVPGYRVAGKTGTARVAGKNGYKDRKYTASFIGIAPVSKPK
                     FVVAVIIHEPSRKGYYAASVAGPLFAKVMSGALRLFNIPTDEPVS"
     gene            11042..12493
                     /locus_tag="AC06IO_001940"
     CDS             11042..12493
                     /locus_tag="AC06IO_001940"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIO9875118.1"
                     /translation="MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAAS
                     DGRLYIEKAVASGAVAVVYEPNNLPQGITLPTAIPCIAIPQLAEKLGEIAKRFFNNPS
                     QFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
                     LQRLMHQYKEEGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYG
                     AAKSRLFERAELQWAIINQDDDFQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDIT
                     GTEIEVKSPWGHQLLKIKALGKFNIYNSLAVYSSLLASGHNPEKVVQVMAELKAAPGR
                     MEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAA
                     SMYADNIVITSDNPRSEDPEIILNEIACGIPASAPVSKLVNREEAIAYALRKAEHNDI
                     ILIAGKGHESYQQIGNTKHVFSDQEVVRRLMNN"
     gene            complement(12538..13590)
                     /locus_tag="AC06IO_001941"
     CDS             complement(12538..13590)
                     /locus_tag="AC06IO_001941"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HIO9875119.1"
                     /translation="MNILADALLPGLDSAFPPPFTVTLYHKADEIPELLHHKDVLLCR
                     STLKINGDLLKNHQIKVVATATSGTDHIDFPFLESQNISIIDAKGCNAISVADYVVAC
                     LAYLDKQQLIQGKTAGIIGLGQVGTKVYERLNAAEFQLCLYDPPKATRDTSFQSCSLE
                     DLFECDLLCVHAELHSDAPYPSLNLINRDFLKELKPGCIIINASRGGIVNEEALLHLG
                     SAILYCTDVYNNEPHIDSRIVSKATLCTPHIAGHSLEAKFAAVAIVSRKLHQMLGLPY
                     PQFATPEKPYRLNENSDWRELALSIYNPIHETLELKHAGNLSSAFLTLRKNHHHRHDF
                     TTYFDSDSIKKYPLLG"
BASE COUNT         4169 a         2336 c         2930 g         4180 t
ORIGIN      
        1 aattgataaa ttattagcgc gacaggcatt tcgcaagaga tttgcaagcc gtgcaggtat
       61 ggagttaaat ggagatgatg agacaacaac gtaacttatt acttatccct tttattttat
      121 gttttattct ggcaagacct gtttttagtt taaacctgga tgctctcctt ccagatgaac
      181 gaaatacagt tgaagtattt caaaaagcct catccaaagt tgtttatgtt catcgattgg
      241 caaatgctac tgttcagcgt cgttactcat tacaaaaaac acatattcct gatggggcag
      301 gctccggtat tatttgggat aacaaaggcc atgttgtaac caattttcat gtcattaacg
      361 gtgctgatga catagcaatt actttgggaa atatgactgt ccctgccaaa gtgattggtt
      421 cagagcctcg caaggacatt gcagtccttg aaattaaatc acctaaggct ctgaattatt
      481 tgaaatcatt tcagcccttt gaaatagtct ctcttaatga tctgatcgtt gggcaaaaag
      541 cgattgcgat tgggaatcct tttgggttgg atcatagttt atcaaaggga gtgatttctg
      601 ctttaggtag aaaagtacca ggtataggtg gggtaacaat ttatgatatg attcagacag
      661 atacccctat caatccgggt aattcaggag gtcctctatt aaatagcgca ggccaattaa
      721 ttggtatgaa caccatgatt tattcacgat ctggttcttc tgcaggtatt ggtttcgcag
      781 ttcccgcgga agatattcag aaaattgcat ctcaaattat taatcatggt cgagtggtat
      841 tgtctggtat cggtattcaa cgagttgagc cacatcttgc ggaaaggctg ggagttaaaa
      901 agggtatttt gattgctgat gtggtgcctg gaactcctgc agacaaattg aagttacgag
      961 gtactcacag agatcaatgg gggcgtattg ttttagggga tgttattgta ggtgtgaatg
     1021 cacatcctgt cccaaattat gatgccctgt ataatttgct cacggaaatt aaggtgggag
     1081 agcaaattac tgtttcaata ataaggcatg gtaagaaaat ggatgtcaaa atgaaaacaa
     1141 ttgatattgc agcgctttaa ctctcttcat tttttctaac tattatttcc ccggaaaaga
     1201 attatgcctc aatccggggt tcactcactt ttcttaatgc tatatgatat agatcaataa
     1261 atagaatgag gctatcagga tgttactaaa tagagcggat tctcttttgg tcttgattga
     1321 tgtacaggaa aaactaaccc cggcaatatt aaatctcgat ctttttttag ctcgatgtga
     1381 atggttacta aaattagcca gaaaactgga cgtgcctgtt ctagctagtg aacaataccc
     1441 taaaggttta ggtcgaaccg tttctccttt aagctcttat atcaatcaag aagaatgcat
     1501 tgaaaaaatt catttctcat gcatgcaaga accacaatat attaacctcc tgaaaggata
     1561 taataaaaac cagcttattc ttatcggaat tgaggctcat gtatgtgtgc ttcaaacggc
     1621 catagaaatg aagggctatg gattcgaggt ctttgttgtt gtagatggtg tgagcagtcg
     1681 ctctgagttt gatttaaaat acggtctaaa aaggatgaaa cagaatggga tccatttgat
     1741 aaccgctgaa atggtactgt tcgagtggtt aaaacaagca ggtacgccag aatttaaagc
     1801 gattagcaaa gaatttttac aatagaccat ttgcatggcc tgattatttc cttttattgc
     1861 tgtgttgcaa acgtttctgc ggtactcatg tactgtatgt aagcttcctt cctcaaagcg
     1921 gttttgtgct ttgccttaaa aaataagcag gtttgccaag aggtctaata gaatactgtt
     1981 tttaagtaga ataagaaagt tggaaattga aaccgtagaa ttttggagag aaaatgaatt
     2041 tcgataatca gattgcacta gtaactggtg gtgtctcagg tatgggtaag gcttgtgtac
     2101 aatacttaca acagcatgga atgaaagttg tggtctggga taaacaggaa ggcacctcaa
     2161 atgaggcgga attgtatgtt gcttgtgatg taaccagtga tgaatctgtt gaaaaggcaa
     2221 tgcgacaaac tatctctcag gtaggagtac caagggtttg tatcaattgt gcgggaattg
     2281 ctccggcaaa acgcatggta ggaaaagaag ggccaatgcc tttggaatca tttagacaag
     2341 taatagatgt caatttaata ggtaccttca acgtcatgcg aatagctgca catgccatgt
     2401 caggattgga gttagacaac aaatctcagg aacggggtgt cataatcaat acagcttcca
     2461 ttgctgcttt tgagggacaa ataggacagt ctgcttatag tgcgtcaaag ggaggaatag
     2521 tatcaatgac cttgccagca gcacgtgaat tagctcaatt tgcaataaga gtaaatacaa
     2581 ttgcgccggg gttaatagca actcctttac ttttaaatat gcctcaagaa gttcaggaca
     2641 gtttggttgc aacagtgact tttcccaaac gcctgggcag accagaggaa tttgcttcac
     2701 tagtaggtca tattattgaa aaccagatga ttaatggaga ggtaattcgt ttggatgcgg
     2761 ctctccgtat gcgttgagcg gctatgcctg gcgcatcata ctggagagct attttaaaaa
     2821 atactagggg ttatatttct tatgcttctt catggtgact gctttcggaa ggggtttcca
     2881 aattcccgtt agaagtgtaa acactcacag cattagattt atttcccgac aaggcgttga
     2941 caatttgttg acgggtatac atgttattcg ctatgacttg tccattaatg gtatttaact
     3001 cacgacatag gtttaatttc tctgcaagag tattatttaa atgattgatt tgaatgccat
     3061 cttctgtagc gcaatttttg agaaaatcag acaacaggag tccatgaggt ttgtcgccgg
     3121 gatttgattc ttttataaga tctacacgtt gttttgcact agcctctaat ttagttgata
     3181 attcctgttt tttctcagct aatgtctcca gctgattgaa ttgactggta gaaagtatct
     3241 ctttttcttc agataataaa gtattgagtt cttctaccca gctaatttcc tgttctaatt
     3301 gggttattaa tgtttttgct ttattgttat tattcatcgg gtcgccctgc agtttaaacc
     3361 atctctacac tatttaacat gccatgtgca atcttgctgc tatcaatttc atactgcccg
     3421 gattgaattt cagctttgaa atacaataca cgagcctcat tgatttcagg taaatcttta
     3481 agagatgctt ttaaagcttc cagttgcttc gatgtactac ttaaattgac gctatcagta
     3541 gagttatttt ccagaacagg gcttttgact tccttgtgat ttgcattgat acgattgtcc
     3601 atatcgatat gccttaaatt ggcagagtca ttaatttggt taaccatatg aatatcctca
     3661 atcgtattta tcatgattat cggctccttt tgcaatttct ttagtaaatt aaggttgatt
     3721 tttaatcctt tttccaaatt ttttcttaaa aattaaaaca ttagttaaaa aataactttc
     3781 acggttttct ttcctgtgac ttgagcttca ataattcgtt ttgaggaaag attttttact
     3841 tttacagagt ctcctagcgc tccctcatcc attgcaattc catccatact cactgttaag
     3901 ttgtcatgag cggctattat agatacctgt tctcctttat ggaccagttt ggctaattca
     3961 atattgaaag ggtttagtgg gctattaggc gtgatatcat gcttgcaaac caatccaata
     4021 agctctttac tgtcagtaaa atatccttgt ttcagttttt gaacatcaag ctcggtttga
     4081 tatatatctg aattactaat ttttgtacct ttcagtaaag ctcgttttgc cacaagtaca
     4141 gatttaaata tagttatttt gacaggtata tataaggtcc agtgattgtt ttcctccaaa
     4201 catttgattc ccatggtact ggtattgagc atgggtgttt gataagggtt aaatacttca
     4261 agtttattat catcacaaac ttttaagttt aggcgaggat caatattatc cgccgtaacc
     4321 tgaattttac cttcagtata gtttgataat tcattgaggg cgtattgttc tattttattt
     4381 tttaataatt cgagagactg acttgattct gaaaataaac tggtgcttgc aaaaaataag
     4441 aaaaagctta gtatgcactt tttcacgaca taaactccct tagtctattg attttttata
     4501 ttgaaacgca gcgtgtccca agcaagtatt gcgccaattt taggagtata tcatgagacc
     4561 ttgttatttt gtttgtatat tggctttatt tatgtttgcg atgccagggt actctgagaa
     4621 tcatcatcct caagagtttc taaagtcaat tgctggcaca aaggatgaag gggaaaaaat
     4681 ttttaatcat ttttgtgtta attgccatgg aacgaaacca atgatttctt taggagcccc
     4741 tgaaattgga aatgaaagag catggggggc aagattgaaa caaggaatgg aagtgctttt
     4801 taaacacacc gatgaagggt ttaatgccat gcctcccaga ggaggatgct ttgaatgtac
     4861 tgataaacag ctgattctag ctatcattgc gatggttccc aaacaggctc aaaaaggact
     4921 attaaacaac ctgttagatc ataaagaata caaacagtaa aaaattttta taaaaaagcc
     4981 taaacttatt caaactgctc ccgatatagt taaaaagaga aagagggaga tagaaagtga
     5041 aaaaacaaat actgttagtt ccggtttgtg tcttggctgc ctcttgtgca tccatggata
     5101 gatcaacggc catagtcgat gatgcgggtt atacccatta cactatgagt atggagtccg
     5161 atagaaaggg ttctaattac tttcctgaga aaagacaggc cacaggaaaa aaagtattta
     5221 tttttgatcc taaagctact gcctgggcag cttacgatgc tcagggtaac cgagttaaaa
     5281 ccggtagtgc ttcaggggga aaagattttt gtgaggacgt tggcagaccc tgtcggacgg
     5341 taacaggcag ctttaaagtt tattccaaga aaggcgagga gtgtacctcc agtatctatc
     5401 caatagaaac tggcggtgga gcaaaaatgc cttattgcat gcattttcat ggtggttatt
     5461 caatacatgc tgcctatgaa gttcccaact ataacgcaag tcacggctgt atccgggttt
     5521 taccaagcgc tgcaaaatgg ttaaatcaaa actttattga cgtaggaact tcggtaatcg
     5581 ttaaacctta ttaataagta aaaattctcc actactcccc gctaattgcg gggttttttt
     5641 attggatgat tttattatct cgtgcagaca agaggataat ggtttcgtaa gaagtccagc
     5701 taaagtttgg gttgggagca aagcggatct aacaataatt tgaatcaata atatatagaa
     5761 tgctctcaat acagggctat ttctttccgg gattgctgaa ttattatctt agacctataa
     5821 tcacaaatca ctagttatgc catttaaaaa gtagatttaa taatgattct gtgtattgat
     5881 gtaggaaact ctcatatata tggtggagta tttgatggag atgagattaa actccggttt
     5941 cgccatactt ccaaggtaag tacttcggat gaattgggca tctttcttaa aagtgtttta
     6001 cgtgaaaaca attgctctcc cgagacaatt agaaaaattg ctatttgttc agtagtacct
     6061 caagtagatt actcattgcg gtcagcctgt gtgaagtatt tttctattga cccgttttta
     6121 ttacaagcag gagttaagac tggattgaat atcaaatata gaaatccggt tgaggttgga
     6181 gctgaccgaa ttgcaaatgc tattgccgcc actcatagtt ttcccaatca aaacattatt
     6241 gttattgatt ttggtacagc aactactttt tgtgccattt ctcataaaaa agcttattta
     6301 ggtggagcta ttttacctgg tcttagatta tctgctgatg ctttgtcaaa aaatactgca
     6361 aagctacctt ctgttgaaat tatcaagacc gagagtgttg ttgggcgttc taccatagaa
     6421 agcattcagt ctggagtcta ctatggtgtc cttggtgcat gtaaagaatt aattcaaaga
     6481 atacatcatg aagcgttcaa tggcgataaa atactaattt tggcaacagg aggatttgcg
     6541 tctttatttg ataagcaagg tctttatgac catcttgtgc cagatttggt tctccagggg
     6601 ataagattag ctgctatgat gaatactgcc tgagcagtaa aaggtttttg ttaatatcag
     6661 atttatttct gtttattcta atggagttta aggggtgttt tgtattctgg taagttaatc
     6721 aaaagagagg atttcgagtc ttataaagca ggaaaccctt ttgctcagga tttccatttt
     6781 tagcgtttta cctcccgctt tttctattag aaaaactctg ggctcttcct tctgtttcat
     6841 taatcaattc aagatcaacc tatgaaccaa acaaattcta gaccgctcac ttgcataatc
     6901 tgtctctttc attttattgc tgcgttggaa atgttcttgt ggggctcatt tactctggca
     6961 aaccctgctc ctcaaaaaat ctgctctttc cttgaagcaa aataatcaga tgattcgaga
     7021 gaacttataa ctgtgagcat acatttggtt acaaatcttt cttaaagatc aaataaataa
     7081 caagtttggg atttgcctct ttaatccata aaaaaaatta ctccaaaagt ggtatttcag
     7141 cttgacgtgt gtagaaatgt gttcctatag tgtagaaaag tgggttaaaa taataaaaaa
     7201 gtggagaatt gtgggagaat aattctcctt cacttgggaa ataatatgtt tcgtggaatt
     7261 aatgccatca ccatagacac taaagggcgc ttagctattc cgacgcgtta cagaagtgcc
     7321 ttgggtgcag aggataaaat ccctctcgtg gtgactattg ataccgaaga aacctgctta
     7381 ctcctttaca cagcagcaca atggcaaatt atcgaagaca atttgcaaaa gctaccaagt
     7441 tttaatgctg cagcaagacg aattcaacgt ctgttaatcg ggcacgccac tgatgtggag
     7501 gttgatgcga atggacgagt acttttaccc acagttttaa gaaattatgc aaagttagaa
     7561 aaagatgtcg tgatgatagg ccagggaaat aaatttgagg tttggaataa agaattatgg
     7621 gaatccaagc gtgagcaatg gctagcagaa gaagcgtcca tgacggatgg gttacccgag
     7681 gaaatgaaaa cgttttcttt ataacggaaa gagaaatggc aaagcatcaa tctgttttat
     7741 tacatgagtc aatcaaaggt ttggctataa aagctgacgg catctatttt gatggaactt
     7801 ttggccgagg aggccacagc cgcgagattt taaatcattt aagcgataaa ggaagactgt
     7861 ttgcaataga taaggacctt gatgcggtgc aatatgccaa ggattatttt ggtttggata
     7921 aacgatttca aatttttcac ggttcattcg cgcaaatcaa ggaatttgcg tcacaagcgg
     7981 gagtaattgg ggccgttgat ggtattttgc ttgatctggg agtgtcttca cctcagttgg
     8041 acaatccaga aagaggtttt agttttatgc tgcaaggccc tcttgatatg cgaatggatt
     8101 taactcaatc aattaatgct gcgaattttg tcaatgaagc agaagtcaac gagctggctc
     8161 atgtattcag ggcttatggt gaggaacgat ttgcaggcag gattgcaaaa gctattgttg
     8221 atgcaagaaa actaaaacca ataacaacta ctttggagtt agcagaaatt gttaaagaag
     8281 caaaccccaa atgggaaaaa cacaaacatc ctgcaacccg ggtatttcaa gcgattcgaa
     8341 tacatgtcaa tcaggaatta accgatttga gtaattgtct agagcaatgt ctggacgtat
     8401 tggggccaag aggacgcttg gccgttatca gtttccactc tttggaagac cgaatagtca
     8461 aacaatttat gagagataaa gagcaaggta acaggccgcc tgttgaagtc ccaatcaaat
     8521 acgaagaact aaagaccaat tttaaaagag taggtaaagc agtgaaacca cagagtagcg
     8581 aaataaagga aaatgttaga tctcgaagtg cagtacttag gataggggag aaattagcat
     8641 gaacgctgca gcgaaagtca ttaatcaagg taatttattt aatggtcaat tggcagatat
     8701 gcaaatgtca aaatcactct atatgttgat agtattgtta gctgccgtac tagtcagtgc
     8761 atttgctgtc atatatagca ctaattccta cagattaact ctcagtcaag tgcaacaaga
     8821 ggaacagcaa acccactttt tacaattgca atggggacaa ttattattgg aacaggccag
     8881 tttggcaact ccagcccggg ttgaggaatt agccagagaa aaattgggaa tgactttacc
     8941 aacttctgca aatacgtact tactgcatcc tcaataagtg agtcgtttta gaggagttaa
     9001 agtgtttcca tattttgagt tataggtaag agctgagtac ctggtgaagg ataatctatg
     9061 tccaagagca tcaaattaaa gattgaataa tgaaatttca aaggatttga tagagcaatt
     9121 aggcgcaatg cggaggaaat cacctcgaaa aaattgaaag cttcatgtac acttacacgt
     9181 cattcatctt ctttccagga cgtcatggaa gactaagtgt aaaatagcct gataaattcc
     9241 aggcaggtat acgtgcctta tgtttaactc taaggtaatt ttgagaaaga ggtttcaagt
     9301 atattttcac taatttattg ttgcgtgcat agctaatact atttatgagt agtcatatat
     9361 gaaaaaaaca caccattttg ccaggctggt tactgtcgct gtttttttct ctttgatttt
     9421 ggctgttttg atatggagaa tggttgatct cactgttctg cataggcaat ttttgcaggg
     9481 ccagggtgat gcgcgcagca tccgagttat agatattcct gcctatcgag gtatgatcac
     9541 cgatagaaat ggtactccgc ttgctgtgag cacacctgtt gaatcggttt gggtcaatcc
     9601 aaaggaattt tctccggatg aagaacaatt tatgcagtta gctaaatacc tgaatttggc
     9661 acctaaagaa ttaagtagaa aaattgtccg ggccgaaaat cgggagtttg tgtatttaca
     9721 aagacagtta cctccacccc tttcgaaaaa gatcgaagct ttaaaaatcc caggtgttaa
     9781 ttttcaaaaa gaattcaaac gttattatcc agattcagat agtatttccc cattaattgg
     9841 atttaccaat gtcgatgatc aaggattgga ggggattgag ctggcctatc aagattggct
     9901 tatgggagtc gttggtaaaa aaagagtaat caaagatcga ttaggacgag ttgtcgaaga
     9961 tctgggagtt ttgaaggaac cacgcccggg acatgagtta actttgagta tagatagacg
    10021 actacaatat ttagcttata gcgaattagg taaaacagtt gaagagtttt ctgctaaatc
    10081 tgggtctgta gttgtaatcg atgctgaaaa tggtgagatc ctggctgtgg ctaatgttcc
    10141 atcttataat cctaacgcac gtgggcatta tgataaggaa acctaccgca acagagcctt
    10201 caccgatact ttcgagcctg ggtctgtcat taaatcattc agtatagcca gtgctttgga
    10261 aacaggctta tttactccgg ataccattat tgatactaat ccaggatgga tgactgtcca
    10321 tggccgaaca gtaagagata tccataatta tggtgtttta gatgttaccg gaatattaca
    10381 acattccagt aatgtgggtg ttacaaaaat ggtattatcc actccagctg aacaattaat
    10441 cgggtttttg caaaaatgtg gttttgggca acgctctgaa ttggcatacc cgggtgaaag
    10501 tgaaggaggc atagtaaaag ccaaggatgc aaacccattc gttttagcca cattaggttt
    10561 cggttatggc ttgtctgtta ctgcactgca attggctaaa gccaatttaa tttttgcaaa
    10621 taaaggacga ttaatacctg tgactttgat tcacaatgat acccccagta atggcattca
    10681 agtgatgcaa cctaaaaccg ctgaaaatgt attattaatg atggaagcgg taatgaataa
    10741 gggtacaggt aagggagcaa gggttcctgg ttatagggta gctggaaaaa ctggaacagc
    10801 acgagttgct ggaaagaatg gatataaaga caggaaatac acagccagtt ttattgggat
    10861 agcaccagtt tccaaaccaa agtttgtagt cgctgttatt attcatgaac cttcacgaaa
    10921 gggttattat gctgcctctg tggcagggcc cttatttgct aaagttatgt caggtgcttt
    10981 acgcttattc aatattccta ctgatgagcc agttagttaa agaagaatag gagccaattt
    11041 tgtgaaatta acccaattat tagaaccgtg gattaaaagg gacattattg atggcactgt
    11101 gagtgacata aaaaatgaca gccgcttggt gaaagaaggg gatttatttg tcgcctatcc
    11161 aggggcagct tccgatggtc gattatatat agaaaaggcc gtggcatcag gtgctgtcgc
    11221 tgtggtttat gagcccaata atttacctca gggaattact ttgccaacag caattccctg
    11281 tatagccatt ccccaattag ctgaaaaatt aggggaaata gcgaaacggt tttttaataa
    11341 cccatcccaa tttcttggtt taactggggt aactggcaca aatggcaaga cgactattgc
    11401 ttatcagttg gcacaggcac atgatttact cgggcagcac tcggcttaca tcgggactat
    11461 aggacaaggc aaggtggatg ctttaaaacc tttggaaaat actacgccag atgctctttg
    11521 tttgcagagg ttgatgcacc agtacaaaga agagggcatt aaacaggttt gtatggaggt
    11581 atcttctcac gcgttgagcc agcatagagt cgactctctc gaatttagtc aggctatctt
    11641 taccaattta acgctcgatc atttagatta tcaccaaact atgcaagctt atggggctgc
    11701 gaagtcgagg ttgtttgaac gagcagagtt gcaatgggct attattaatc aggatgacga
    11761 ctttcaagat ctcatgagca atgtgctaaa gactcatgta aaaaaagtga cttacggtat
    11821 gcatcaaaat tgcgatgtga aagcgctcca atggaatatg gatatcaccg gaacagaaat
    11881 tgaagtcaaa tctccttggg ggcaccagct tctaaagatt aaagcactgg gtaagtttaa
    11941 tatttataat agcctggctg tttatagcag tttgcttgcc agcgggcata atccagaaaa
    12001 agtagtgcaa gttatggccg aattaaaagc ggctcctggc cgaatggaaa ttgtcgctca
    12061 atccccatat gttcttgttg attatgctca tactcccgat gcattggaaa atgttcttat
    12121 caccttaaaa caaataaaaa aagggagatt gtgggttgta tttggttgtg gtggggacag
    12181 agataaaacc aaacggccca ttatgggaaa ggcagccagc atgtacgctg ataatattgt
    12241 cattaccagc gataatccga gaagtgaaga tccggaaatt attctaaatg aaattgcttg
    12301 tggcattcct gcctctgcgc ctgtctctaa actggtgaat agagaagagg caattgctta
    12361 tgctttaaga aaggctgagc ataacgatat catattaata gcaggaaaag gacatgaatc
    12421 gtatcagcag attgggaata caaaacatgt tttttctgat caggaagttg taagacgttt
    12481 gatgaataat taaaatttcg ggacaggggt taattattag aaacagacat caacacttta
    12541 acctaataat ggatactttt ttattgagtc agaatcaaaa taagttgtga aatcatggcg
    12601 gtgatgatga ttcttgcgaa gagtcaagaa tgctgaggat agatttccag cgtgttttaa
    12661 ttctagtgtt tcatgaatcg ggttatatat tgataaagcc aactctctcc agtcgctgtt
    12721 ttcatttaac ctataaggtt tttcaggggt tgcaaattgt gggtatggaa gaccaagcat
    12781 ttgatgtaat ttacgactga cgattgcgac tgcagcaaat ttagcttcga ggctgtgtcc
    12841 tgcaatatgt ggtgtgcaaa gagttgcctt ggagacaatc cggctatcta tatgaggttc
    12901 gttattgtag acgtcggtgc aataaagtat ggcagagcca agatgcaaaa gggcttcttc
    12961 attaacgata cctcctcgag aagcattgat gataatgcag ccaggtttta attccttgag
    13021 aaaatcccga ttaattaagt taaggcttgg gtagggcgca tcgctatgta attcagcatg
    13081 aacacataag aggtcacatt caaaaagatc ttcaagagag cagctttgaa aagaagtatc
    13141 tctggttgct ttgggtgggt cataaaggca taactgaaat tcggcagcat ttagtcgttc
    13201 ataaacttta gtacctactt gccctaaacc aataattcct gctgttttac cttgaataag
    13261 ttgctgttta tctaaatagg caaggcaagc aacaacatag tcagcaaccg atatggcatt
    13321 gcatcctttg gcatcaataa tagatatgtt ttgtgattct aaaaaaggaa aatcaatatg
    13381 atcggttcca ctggttgctg tagcaacaac ctttatttga tggtttttta gtaaatcgcc
    13441 attgattttt agagttgaac gacaaagaag aacgtcctta tgatgaagca attctggaat
    13501 ttcatcggcc ttatgataca aggttacggt aaatggtggt ggaaaagcac tatccaatcc
    13561 tggcaataaa gcatctgcta aaatattcat aaattaaaaa tcgcgcttaa aatat
//
LOCUS       DBNCWK010000078        13583 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.078, whole genome shotgun sequence.
ACCESSION   DBNCWK010000078 DBNCWK010000000
VERSION     DBNCWK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.078"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            20..1471
                     /locus_tag="AC06IO_001942"
     CDS             20..1471
                     /locus_tag="AC06IO_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIO9875120.1"
                     /translation="MKFRTKLFLGLLLIVITTNGIFLFLSYQYGKETVYKEVGSSALS
                     IAAATAVLMNPEDIQKFNLDTSANSPLYKDLEKKLLEVRNANRRKDVYVNFIYSLFPD
                     SNNPKVLRFAVDPEENIIDKTELGTVMKLKTESGQTINVNYESPIILPEFVEDIWGTW
                     LTALYPIKDAQGKYLGNIRVDVDAGSVNQRFHFLLYSGIAVFISTFLFAMFLGWLLFQ
                     WFNKPLLKITTALKNIAQGDLEQHLDIRTKDEFAEVGRVINEMTEGLRQRNMLQVSLT
                     RFISHALAEKIVKSGELPQVFSERRKVTIMVCDIRDFTTISERIKPELVVDFLNHFFE
                     KMIEAITSQHGILDKYLGDGFLAIFGSPDDDAYQEDHAIHAALKMREAMKSLNADWAK
                     ILGTEIQIGIGINTGSAIVGNIGTDIHMEYTAIGDTVNLASRIENATKKLNTDILISE
                     YTYIATDHTAFQFIELGEISIKGRVHKVKVYTL"
     gene            1653..2792
                     /locus_tag="AC06IO_001943"
     CDS             1653..2792
                     /locus_tag="AC06IO_001943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875121.1"
                     /translation="MINENADSEVLSEREKLYLIKFYQGLRQLILNHHAQSINEKKPL
                     LIVLGEEHKNFPVVIIKEMINEIIKEINLKNVYVELDDQALKELYFQPSEIRIFITDT
                     EQGGLTVIPVDDQSRTSHDGSMTSMNKRDQKIVSEVNTKGEGGFLSVGFEHMEGLLAN
                     KNGASLSKDKFHILAINVAKPISLEQSVEMAKGSTAEEEKALISRQQFYSKDSRIIQC
                     VPDGQMSQSIHNKCDIASLRGKLNIKNLVAEISQQNQINYSRQHAGFFSQNKSRHHLP
                     VHSGIFIEQNHGQFQLHFNKDHLEKAKLLILQQFGEGGAGYFDADLAKLDHCRNNGLD
                     FVMEFPTLELLKASIEKIASPYLELKKELETIVSDFEYNKPSFEY"
     gene            complement(2928..4487)
                     /gene="ravO"
                     /locus_tag="AC06IO_001944"
     CDS             complement(2928..4487)
                     /gene="ravO"
                     /locus_tag="AC06IO_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavO"
                     /protein_id="HIO9875122.1"
                     /translation="MPTGIVLSSQSPEATTSFPRRGGTAAGINHVTGFPVIKMHRDAQ
                     ARPDERKYFNQIKDGSLVFITGHSNTALDHLTGDYVPPLATPVQPMSTEWTYKQFRDL
                     ILKNGSLKAGDTITVVLWACNAGEGGLSSGAGLLGRLFREKQINTRVIASAATTMRFD
                     GHYSPTPEGTPAMAFITENGSKDIRIFDFTEEATIEWKNKGRLYVTCNGIEGYNLYSP
                     KQLESLESEKIYKVMEEIFVDRHYKENIRTGEEVKQYFSKSKAEFILRWSSANENDTE
                     NKYVFIAASFQASDGIHSIRYGINKNGELFSINTTSNKVTSIDILPQGVMATLTQHIT
                     QNKELIEKALHEKGAQMTPSPKLQHRVKRKKSDHTELLILKGEKEPLKPVQTDTLSSK
                     VPASPGWPTQENQDFLIFVTPLQVQELLKSHGETKKEKHEKKIRLGHEKTIGDEKTRK
                     PVSKHKEPFGENEEPEMDLSKRQRIPVAQRKRPQGRRHEFFSNSPKNPDEHKTEKDKT
                     ISPSRNPTKGG"
     gene            complement(4915..5379)
                     /locus_tag="AC06IO_001945"
     CDS             complement(4915..5379)
                     /locus_tag="AC06IO_001945"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIO9875123.1"
                     /translation="MDFIKRTIELARENVEHGGRPFACLIVQNGKILAEATNTVAQTH
                     DPTAHAEVNAIRIATAKLGCEHLYDCDFYILAHPCPMCLAAMYYCSPRKVIFITTRDE
                     YKTYYVDDRKYFTLENFYQEINKPWRERKMPMEHRPDKEAIEVYKMHQKKKS"
     gene            5461..6969
                     /locus_tag="AC06IO_001946"
     CDS             5461..6969
                     /locus_tag="AC06IO_001946"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIO9875124.1"
                     /translation="MTKKRIDRLIFSEIECNMKKCSMVIAGQPITYERFFVTVNRIAT
                     HFQNKVPSGSRIAFMLPNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLQDAQPI
                     YLIIEASKLFLLEKINWSATGIKKIFVLGEDRSHPYFSFDDLLTEPEKLPKQSISYET
                     PAVIFYTSGSTGQPKGVIHTLSSIAAMLDSTSLALENITADDKMIICEPQCHVSGFIE
                     TFSTLSREGTALVHDGFDMDWYLATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSF
                     RGIYTGGDALPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVNG
                     VTLRLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDE
                     QGYFYYKGRIKNIIIRNTSNIMPGEVEAAIYKHPAISAAAVIGVPDEAEGEVPIAFVV
                     VKKSNQLTKEELYSFLLEQIAQYKIPEKIYFIDEMPLTNSGKINHKKLYDYL"
     gene            complement(7003..8697)
                     /locus_tag="AC06IO_001947"
     CDS             complement(7003..8697)
                     /locus_tag="AC06IO_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIO9875125.1"
                     /translation="MHDALPLITTIAMGFALALILGFIAVRLKMPALVGYLLAGVTIG
                     PFTPGLMANVEIAQELAEIGVMLLMFGVGLHFSLSDLLRVRKIAIPGAIVQMIVATIL
                     GGGTAIMWGWDYGSALIFGLSLSVASTVVLLRALEERGTLESINGHIAVGWLLVEDLA
                     MVIVLVLIPPLSHWLGDNSSVTIHRPLWIVLGLTLIKVTTFIALMFLVGRRFFPKLLW
                     QIARTGSRELFTLCIITAAISIAYVASKLFGVSFALGAFFAGMIMRESSFSHRAAEES
                     LPLRDAFAVLFFVSVGMLFNPAVLVEQPLQVLTVVSIIIIGKSIAAFILVLAFRYPIQ
                     SALIISASLAQIGEFSFILAELGIRFDMLPEAGRSYILAGALISIAINPLIFKLINPV
                     HAWVQSHPKLVGILERTDDPLAELPRTTKEEYLSGQVVLVGYGRVGMHIAQLLSKKVV
                     PFVVIDENRELIERLRASGIPAVYGDASEPSVLIQGHIARASMLMTVTSDTIHVRKMI
                     QCAYKLNPDIEIVVRTHNEEEAELLQKEITGKVFFAEGEIAKSMGHYALNRYGKSP"
     gene            complement(8958..12389)
                     /locus_tag="AC06IO_001948"
     CDS             complement(8958..12389)
                     /locus_tag="AC06IO_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIO9875126.1"
                     /translation="MKILKTTEKKSRKPTPKENRALPLQLVQNTEPDRTYYLRSTVEG
                     IGSFLSTRVKELYNTLAMTFSLDSMDWSSFKKYIWPTISSGAALLLGDYLTRSMSEYQ
                     TGSPEYDFIFKFLFGTAILGYCALNRITDRKFTSQVELSAAVASEVMAITYMYSSVFK
                     GTTYLLNDSISDEYSLIAGLGTSALLVPSGISYLTQKIQEFLVRIQYNKGAQRSDTAV
                     ISSGLTPISNLYFQVNHFIASELMISSRAFQLGLISHNIRYADRIMQKFRNLPALLAD
                     LAPSTIKKMRIQQQKLGHDYEYNHKLHQVLRFTADGPMFISIPRYQLRTGDLVYCDSS
                     IDLACVPVSGELLALRRDLKGEFTQTLEQKKFSVNLKAQNGEDVWIEHETKPHFTSHY
                     KKVDLHAVRDGKQAGVLVGDKLNLYGNDNIFIQIKPEKELLLSSNYEKKAVINEIIAE
                     RKQRSVLYSILGSVIMAAFLQRDITAMPAETLRLMFTLFQTMIPFSEAFLRETVNSRL
                     MKKLNSELGDQPFGTIDALRVVDLCNALGGYYRDRFPNGVAIISDKTGTLTTTRMDVL
                     GLWTNDMAPEVQKTLKEKEDLLLPEETQWLQTFEVFCNAFTNNKKELEPEEHAILELF
                     QSLLDNRQCLEVITLGNNHFKKEMISKDIKRETETFHLGLYRTFGGRFTLVQDGSDHY
                     LVFCGVPKQEAFKETPLLKAYTTMQSRTGVLSRDWCLARGKLSEREFTILKELFSKDD
                     KKQIEHFILQNKLLLENLMYYGTFIIDNPVKKGAEKFIAQCREISVPVLVATGDTTKA
                     AENIAKVLCPANAGKIVTLRADQIDEKGVEFLDEENYLSDSTVIFSGINEAILNRFQK
                     LMDREGAKRPVVIFAEMSTEGKGVLARYLKDHHYFVVANGDGTNDVMMMKNSNVVIAH
                     HSDDGTFAPGVDGLTNISEEQLRRLFGSQKTFYELFDINLPKSLFVQKFAPLANSQEK
                     PTMALALKSGKMTFDLAKTVGADVTEMNQQHWYSVVFDLLWLWIAFYEINESSDLPMD
                     NRNINASRLISNTMGIALIIAVFQSLANYALFNESTNLTSMLIMLSFLPLILKSIFSG
                     FRMVQDSLYPQVEVTEVEEEGDTSTNGSVLSYFGTFFSRKPVKQLEGLNNAPALK"
     gene            complement(12701..13396)
                     /locus_tag="AC06IO_001949"
     CDS             complement(12701..13396)
                     /locus_tag="AC06IO_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9875127.1"
                     /translation="MILKAGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLV
                     QIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELK
                     PNVSSFEDLKGLVAGIQKGNTSDEVAQNLLRKNILSDIKYYAYNEIELAIQDLSAGKI
                     GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDKVNLILAELIQNG
                     TLSRLQHKWISTT"
BASE COUNT         4367 a         2639 c         2369 g         4208 t
ORIGIN      
        1 tgctaatggt ggataaataa tgaaatttcg tactaagtta tttcttggct tacttctcat
       61 tgtaatcact actaacggca tatttctgtt tttatcttat caatatggta aagaaaccgt
      121 atacaaagaa gtaggctcat cagccctatc catagctgcg gcaacagctg tcctgatgaa
      181 tccggaggat attcagaaat ttaatctgga tacgtctgca aactcacctc tttacaaaga
      241 tcttgaaaaa aaacttcttg aagtacgtaa cgcaaatcgt cgcaaagacg tgtatgtgaa
      301 tttcatatat agtttatttc ccgactctaa taatcccaag gtgcttcgct ttgccgtaga
      361 tcccgaagaa aacattatag ataaaaccga attgggaact gtcatgaaac ttaagactga
      421 atcagggcag acaattaatg taaattatga atcgcctatc attctgcctg aatttgttga
      481 agacatctgg ggtacatggc tgaccgcttt atacccaata aaagacgcac aagggaaata
      541 ccttggaaat atccgggtgg acgtagacgc cggttcggtt aaccaaagat ttcatttctt
      601 gctgtactca ggtattgccg tctttatctc aacattttta tttgccatgt ttttaggttg
      661 gttacttttt cagtggttta ataaaccctt attaaaaatt acaaccgcac tcaaaaatat
      721 tgcccaaggt gatcttgaac agcatctcga cattaggaca aaagatgaat tcgcggaagt
      781 gggtcgagta atcaatgaaa tgactgaagg attgagacag cgtaacatgc tgcaagtaag
      841 cttaacacgt tttatttctc atgctttagc agaaaaaatt gtgaaatccg gcgagttacc
      901 gcaagtattt agcgagagac gtaaggtcac aattatggtc tgtgatatac gcgatttcac
      961 aaccatttcg gagcgtataa agcctgaatt ggtagttgat tttttaaatc atttttttga
     1021 gaaaatgata gaagctatta cctcgcaaca tggaatattg gataaatatc ttggggatgg
     1081 ttttttagcg attttcggtt cacctgatga tgacgcctat caagaagacc atgctatcca
     1141 tgccgcatta aaaatgcgag aagcgatgaa atcactcaat gccgattggg caaaaatatt
     1201 aggcactgaa atacaaattg gtattggtat caatacaggc agtgccattg tcgggaatat
     1261 tggaactgat atacatatgg aatacacagc tattggcgat actgtcaatt tggcgtcacg
     1321 aatagaaaat gccaccaaaa aactcaatac agatattcta atcagtgaat atacttatat
     1381 tgcgacagat catacagcat ttcaatttat agagttggga gaaatttcta tcaaaggaag
     1441 agttcataaa gtaaaagttt atacgcttta agattaatta tttttaatat aaaacaattg
     1501 atatcagaca caaaacgctc tatgtcatcg ctaccagaat aggctatagc attggttcaa
     1561 tgaatatgta taaaccattc tggattcata acctacacta gctctcataa tatactaaac
     1621 tttgatcata agtgatttgc aatagctaaa ttatgattaa cgaaaatgct gattcagaag
     1681 ttctctccga gagagaaaaa ttatacttga taaaatttta tcaaggatta cgtcaattaa
     1741 tcctgaatca tcatgcccag tcaatcaatg aaaaaaaacc tctgttaatc gtacttggtg
     1801 aagagcacaa aaattttcca gttgtcatca ttaaagaaat gattaatgaa attatcaaag
     1861 aaattaacct gaaaaatgtt tatgtcgaat tagacgatca agctttaaaa gaattatatt
     1921 tccagcccag tgaaattaga atttttatta ccgatactga gcaaggtgga ttgactgtta
     1981 ttcctgtcga tgatcaatcc agaacttctc acgatggttc aatgaccagt atgaataaac
     2041 gtgatcaaaa aattgttagt gaagtaaata ccaaaggtga aggaggtttc ttatccgttg
     2101 gttttgaaca tatggaagga ttgcttgcta ataaaaatgg tgcatctctc agtaaagata
     2161 aatttcatat acttgcaatt aacgttgcta aaccaatttc tcttgaacaa tcagttgaga
     2221 tggcaaaagg cagtactgct gaggaagaga aagcactaat tagccgacaa caattttact
     2281 caaaagattc tcggattata caatgtgttc ctgatggaca aatgagtcaa tccattcata
     2341 acaaatgcga tatagcttct ctgcggggca aattaaatat aaaaaacctc gttgcagaaa
     2401 tttcgcaaca aaatcaaata aattactcaa gacagcatgc gggatttttt agtcaaaata
     2461 aatctcgaca tcatttgcct gttcactcag ggatttttat tgaacaaaac catggtcaat
     2521 ttcaattaca ttttaacaaa gatcatttgg agaaagccaa gctgttgatc cttcaacagt
     2581 ttggtgaagg tggtgcgggg tattttgatg cagatctagc caaattagat cattgccgta
     2641 ataacggcct tgattttgtt atggaattcc ctaccttgga attattaaag gcatccattg
     2701 agaaaatcgc ctccccttat cttgaactaa agaaagagct ggagacgatt gtctctgact
     2761 ttgaatacaa taagccttct tttgaatatt aaacaaagct caagcacgga ttagactttc
     2821 aatacaattg cctctggatt tagaaacaat ttgtttattt agaccattat gttttatttt
     2881 taaaacagga aatcctctgt agacgcatat taaaatcagc ataccagcta gcctcctttg
     2941 gttggatttc gcgagggaga tatcgtttta tccttttctg ttttatgctc atctggattt
     3001 tttggggaat tactaaagaa ctcgtgccta cgaccctgag gtcgttttct ctgtgcaaca
     3061 ggtattcgtt gccttttact taaatccatt tctggttctt cattttctcc aaatggttct
     3121 ttatgtttag atactggctt tcttgttttc tcatcaccaa tagttttttc atgacctaat
     3181 ctgatttttt tctcatgttt ttcctttttt gtttctccat gagatttaag caattcctgg
     3241 acttgcaacg gtgtaacaaa tattaagaaa tcctgatttt cttgggttgg ccaccctgga
     3301 gaagcaggca ccttgcttga gagagtatct gtttgaacgg gttttaatgg ttctttctcg
     3361 ccttttaaaa taagcaattc agtatgatct gattttttac gcttcactct atgttgtagt
     3421 tttggactgg gagtcatttg agctcctttt tcatggagtg ctttctctat caattcttta
     3481 ttttgagtaa tatgttgcgt caaagtagcc attacgccct gtggcaaaat atcaattgac
     3541 gtaactttat tgctagtcgt atttattgaa aaaagctctc catttttgtt tattccatat
     3601 cgaatgctat gaattccatc agaggcttga aaagatgctg caatgaagac atatttattt
     3661 tcagtatcat tctcattggc tgaactccaa cgtaatataa actccgcctt agacttggaa
     3721 aagtattgtt tcacttcttc tccagttctg atattttcct tgtagtgcct gtcaacaaat
     3781 atttcctcca tcactttata tattttttca ctttccaggg attctaattg tttaggactg
     3841 tacaagttat agccttcaat cccattacag gtaacataaa gacgcccttt atttttccat
     3901 tcaatcgttg cttcctcagt aaaatcaaaa atacgaatat ctttgctacc gttctctgtt
     3961 ataaacgcca tagctggcgt tccctctgga gttggagaat agtgaccatc aaatcgcata
     4021 gtagttgctg ctgaagcaat aactcttgta ttaatttgtt tttccctgaa taagcgcccc
     4081 aataagcctg ccccactact cagacctccc tcacctgcat tacatgccca aagaacgaca
     4141 gttatcgtat cacctgcttt gaggcttcca ttcttcagaa tcagatctct aaactgttta
     4201 tatgtccatt cggtactcat tggctgaacc ggagtcgcta atgggggaac ataatcgcca
     4261 gtaagatgat ccaaagcagt attgctatgt ccggtaatga aaaccaggct gccatcttta
     4321 atttgattaa agtactttcg ctcgtcagga cgagcctgag cgtctctatg cattttaata
     4381 acaggaaaac cagtcacatg gtttattcca gcagcagttc ctcctcgacg aggaaaagaa
     4441 gttgtggctt caggggattg tgaacttaat acgattccag taggcattat tcatactcaa
     4501 ttagtttatc ttgaaaattt gagcaaaatt atatgcaatc agatttttat tggaacttca
     4561 aagttgtttt gctttgctca attgcgagtg ctttataatg atcaacggga atcatcttga
     4621 ttttgaagga aactttccat taacttcgtg ctgtctaata taaaaccagc atagtttatt
     4681 ataaaaacat taagattata ttaataacaa tcagcattga aataaaaaat acatttgaga
     4741 tgttttaaaa tttttaagac atgctcaaaa ggtagactga aaaaagcagg aagctactac
     4801 tcaagataaa catgatttat tgaaagtgat caagcaagcc tatgaacttt aaaacataaa
     4861 aattttttaa aggttgaaaa tcaaactgtt ttaaatatta aacagttatt tggactaaga
     4921 cttttttttc tggtgcattt tatatacttc tattgcctct ttgtccggtc tatgctccat
     4981 aggcatctta cgttctctcc atggcttatt gatctcctga taaaaattct caagagtgaa
     5041 gtattttcga tcatctacat aataggtttt atattcatct ctcgtcgtga taaagatcac
     5101 tttcctggga ctacaataat acattgcggc caggcacatc gggcaaggat gagcaagtat
     5161 ataaaaatcg caatcgtata aatgctcaca acctaatttc gctgtagcaa tccgtattgc
     5221 attaacttca gcatgggctg taggatcatg ggtttgcgcc accgtgttgg tagcttcagc
     5281 aagaattttt ccattctgaa ctataagaca agcaaatggt ctacccccat gttccacatt
     5341 ctctcgagcc agctcgatag tacgctttat aaaatccatt ttttcccatt tttagcccat
     5401 aattattaat tatttcccat aatataaaaa gtaacaaatg aatctatcca ggaaaacata
     5461 atgactaaaa aaagaataga caggcttatt ttttcagaaa ttgaatgcaa tatgaaaaaa
     5521 tgctctatgg ttattgcagg gcaacccata acctatgaga gattcttcgt tacagtgaat
     5581 cgtattgcaa ctcactttca aaataaagta ccctctggaa gccgtatcgc ttttatgttg
     5641 cccaatagtc ttgaaatcat agccatttac ctggcctgtt ttcaatcagg atgtattgcc
     5701 atgcctgtta atcgccgcta tgcgccacct gagcttgaaa aggtattaca agatgcccaa
     5761 ccaatttatt taattatcga agcaagtaaa ttatttttgc tagagaaaat caattggtca
     5821 gcaacaggaa ttaaaaaaat ttttgtgcta ggtgaagacc gaagtcatcc ctatttttct
     5881 tttgatgact tgttgactga accagaaaaa ttgccaaaac aatccatatc gtatgaaact
     5941 cccgcggtaa tcttctatac gtctggttca accgggcagc ctaaaggagt tatacataca
     6001 ctttcttcca ttgcagcgat gcttgactcg acttcattgg cactggaaaa cattaccgct
     6061 gacgacaaaa tgataatctg tgagccacaa tgccatgtaa gtggatttat cgaaacattc
     6121 tctacattat cacgagaagg taccgcgtta gttcatgatg gttttgatat ggattggtac
     6181 cttgcgacag tgcgacatga aaaaccgaca ctcgccgtac atcatattga tacctatata
     6241 aagttattag actctggacg ctgtaataca aaaacatttc aatcatttcg tggaatttat
     6301 acaggtgggg atgcactacc cgtggcagtc caggacaagt ttctggcttg cgctggtaaa
     6361 ccaattcaag tgggttatgg aatgacggaa gccatttggc taacagtctg cagaacccct
     6421 gatttgactc aaaaatcctg tattggcaaa ccggtgaatg gggtaacatt acgccttgta
     6481 aacgcagctg gagaagacgt acgttgtggt gaaacaggag aaatactggt aaaaggtgat
     6541 atggtgatgc atagttattg gaataatcct gaagaaaccc gcaaggcatt tataaaaggt
     6601 tggtttaaaa caggagattg cggaattcag gatgaacaag gttattttta ttataaaggt
     6661 cggattaaaa acattattat ccgtaatacg tccaatatta tgcctggtga agtggaagca
     6721 gccatttata aacatccagc aattagcgca gcggctgtga ttggtgtacc tgacgaagca
     6781 gaaggagaag taccaattgc ttttgtagta gtaaaaaaga gtaaccaatt gacaaaagaa
     6841 gaattatatt cttttttact cgagcaaatt gctcaatata aaatacctga aaaaatttat
     6901 tttatcgatg agatgccctt gactaatagc ggaaaaatta atcataaaaa attgtatgat
     6961 tatctttaaa aataatcgaa attgcatcaa atgactcaca cttcatggtg attttccata
     7021 ccgattcaaa gcataatgtc ccatactttt tgctatttct ccctcagcaa aaaatacttt
     7081 ccctgtgatt tctttttgta atagctccgc ttcttcctcg ttatgtgtgc ggacaacaat
     7141 ttctatgtct ggattaagct tatacgcaca ttgaatcatt tttcttacat gaatagtatc
     7201 cgatgtaaca gtcatgagca tgcttgcacg agcaatatgg ccttgaataa gcacagaagg
     7261 ctcagatgca tctccataaa ctgcgggaat acctgaagct cgcaaacgtt caattaactc
     7321 acgattttca tcaatgacaa caaaaggaac tactttttta gaaagtaatt gagcaatatg
     7381 catgcctaca cgtccataac ctaccaaaac aacttgtcca gaaagatatt cttctttagt
     7441 tgtcctgggt agctcagcta aaggatcatc tgttcgttca agaattccaa ctaatttagg
     7501 gtgggattgt acccaggcat gaacaggatt gatcagttta aagataagag gattaatagc
     7561 tattgagatt aatgcacccg ccaaaatata acttcttccc gcctcaggaa gcatatcaaa
     7621 tcgtatgccc agctctgcaa gtataaaaga aaattcacca atttgggcta gactcgctga
     7681 tattatcaat gctgattgaa ttggataacg aaatgcaaga accaatataa aagcagcgat
     7741 tgatttacca ataataataa tactgacaac cgttaaaact tgtaatggtt gttcaaccaa
     7801 aactgcagga ttaaatagca tcccaacaga aacaaaaaat aacacagcaa acgcatctct
     7861 aagaggtaaa gactcttcgg cggctctgtg gctaaaagag gattcccgca taatcatgcc
     7921 agcaaaaaaa gcccctaaag caaacgaaac accaaacaat ttagaggcca cataagcaat
     7981 actaatagct gccgtaataa tgcataaagt gaacagttca cgcgatcccg tacgtgcaat
     8041 ctgccagagc agcttaggga aaaatcgacg ccctaccaga aacatcagcg caatgaaggt
     8101 tgttaccttt atgagtgtaa gaccgagaac aatccatagc ggtctgtgta tagtcactga
     8161 tgaattatct ccaagccagt gtgataatgg tggaattaat acaagcacaa tcaccatggc
     8221 caggtcttca accaaaagcc aacctacagc aatatggcca ttaatcgact ccagagtacc
     8281 tctttcttca agagctctca atagtactac ggtgctggct actgaaagag aaagaccaaa
     8341 aattaaagca ctgccatagt cccaacccca catgattgca gtacctcctc ccaggattgt
     8401 cgccacaatc atttgcacaa tcgcacctgg gatcgcaatt ttgcgaactc gcagcaaatc
     8461 tgataaagag aagtgtagcc ctaccccaaa catcaatagc ataactccaa tttctgcaag
     8521 ttcctgggca atttcgacat ttgccattaa tcctggtgta aatggtccaa tggtaacacc
     8581 ggctaataaa taacctacca atgctggcat cttcaagcgt acagcaataa atcccaaaat
     8641 taatgctaaa gcaaatccca tagcaatagt ggtaattagt ggaagggcat catgcatatt
     8701 tttatgaatc cttttttaac accttatctt tgaccgatga taataacggt atctagttat
     8761 ttaatctcta ccataataaa ttgacaatat atttccttta taaatttaat aaattgttta
     8821 tagatgcttc tcatgtaacc tctctactta attgcttact gggatatgtg aataagcata
     8881 ggagtgctcg tagaatgagc actgcgaaat cgcaataata cagcaataaa gtaaaagaga
     8941 tcgcttatgc aagagagtta ttttaatgcg ggagcattat ttaaaccctc caattgcttt
     9001 actggcttgc gtgaaaaaaa agttccaaaa tagcttagga cagatccatt agtactagta
     9061 tctccttctt cctcaacttc agttacttcc acctgagggt acaagctgtc ctgaaccatt
     9121 ctaaaacctg agaaaatact ctttagtatt agaggcaaaa aactcaacat aatcagcata
     9181 ctggtcaagt tcgtagattc attaaacaga gcataattgg caagggattg aaaaacagcg
     9241 attattaagg caatccccat tgtattggaa atcaatcttg aagcattgat gttccgattg
     9301 tccattggca aatcggagct ttcatttatt tcataaaaag caatccacaa ccataataag
     9361 tcaaaaacaa cgctatacca atgctgctga ttcatttctg taacatctgc tccaactgtt
     9421 ttagccaaat caaacgtcat ttttccactt ttgagtgcca aggccatggt tggcttttct
     9481 tgtgagttgg ctagaggagc aaatttttga acgaacaggc ttttgggtag attaatgtca
     9541 aacagttcat aaaaagtttt ctgggaacca aacaaccgtc ttagctgctc ctcactaata
     9601 ttggttaagc catccactcc tggagcaaaa gttccatcgt ctgaatgatg cgctatcact
     9661 acattggaat ttttcatcat cataacatca ttagtaccat ccccattggc aacaacaaaa
     9721 tagtgatggt ccttaagata acgagctaaa acaccttttc cctcagtact catttcagca
     9781 aaaataacga cggggcgttt tgccccttct ctatccatca gtttttggaa tctgttcaat
     9841 atcgcttcat taatacctga aaatataacc gtagagtcag agagataatt ttcttcatct
     9901 aaaaattcaa cacctttttc gtcaatttga tcggcacgca gcgtaacaat ttttcctgca
     9961 ttagccgggc acaatacttt agcaatattt tctgctgcct tggtcgtgtc tcctgttgct
    10021 acaagaactg gaactgatat ttcccgacat tgggcaataa atttttctgc tccttttttt
    10081 acaggattat caataataaa agtaccgtaa tacatcaaat tttcgagcaa taatttattt
    10141 tgcaaaataa aatgctcaat ctgcttttta tcatccttgc taaataattc tttcaaaata
    10201 gtaaactcgc gttcactgag tttacctcga gccaaacacc aatcacgaga taacactcca
    10261 gtacgacttt gcatagttgt ataggctttt agtaaaggag tctccttaaa agcctcttgt
    10321 ttgggcactc cacaaaagac cagataatga tcagaaccat cctgaaccaa agtaaagcgc
    10381 cccccaaacg tacgatataa acccaaatga aacgtttcag tttctctctt aatgtcttta
    10441 gagatcattt cttttttaaa atgattattc cctaaagtaa tcacttccag acactgccga
    10501 ttatcgagta aggattgaaa taactctaaa atagcatgtt cttcaggttc caattctttt
    10561 ttattgttag taaacgcatt gcaaaacact tcaaaagttt gcagccattg tgtttcttct
    10621 ggcaggagca aatcctcttt ttcctttaat gtcttctgaa cctcaggagc catatcatta
    10681 gtccataacc ctaatacatc cattcttgta gttgtaagag ttccggtttt atctgaaata
    10741 atggcaacac cattgggaaa tctgtcacga taataaccgc caagagcatt acataaatca
    10801 accacacgaa gagcatctat ggtcccaaaa ggctgatccc cgagctcgct attcaatttt
    10861 ttcatcaaac ggctattcac agtttctcgt aaaaaagctt cagaaaaagg aatcatagtc
    10921 tgaaagagtg tgaacatgag ccttagggtt tctgcaggca ttgcagtaat atccctttgt
    10981 aaaaaagcag ccatgataac ggagcctaaa atagaatata aaacacttct ttgttttcgt
    11041 tcggcaataa tctcattaat gactgctttt ttttcatagt tactgcttaa caaaagctct
    11101 ttctcgggtt tgatttgaat aaaaatatta tcattaccat ataaatttaa tttatcgcct
    11161 accaatactc ctgcctgttt gccatctcta acagcgtgca aatccacctt tttataatga
    11221 gaggtaaaat gaggcttggt ttcatgttct atccaaacat cttccccatt ttgggctttc
    11281 aggttaacgc taaatttttt ttgttccagg gtttgagtaa actctccttt taaatcacgc
    11341 cttaaagcaa gcagctcccc agaaaccggt acacaagcca aatcgatgct gctatcacaa
    11401 taaaccagat cccctgtgcg taattgatac cttgggatac ttataaacat tgggccatct
    11461 gctgtaaatc ttaaaacctg atgtagctta tgattgtatt cgtaatcatg cccaagcttt
    11521 tgttgctgga tacgcatttt ttttatcgtc gatggtgcca aatctgctaa taatgcaggc
    11581 aagttacgaa atttctgcat tattctatcc gcatagcgaa tattgtgtga aatgagcccc
    11641 agctgaaatg cgcgtgaact gatcatcagt tcgctggcaa taaaatgatt gacttgaaaa
    11701 taaagattgg atatgggtgt gagcccgctt gaaatcactg ctgtatccga gcgttgcgca
    11761 cccttattgt attggattcg cactaaaaac tcttgtattt tttgtgttag ataagaaatt
    11821 ccgctaggaa ccagcaatgc gctggttcct agcccggcaa ttaatgaata ttcatcagaa
    11881 atactatcat tcaataaata agtggtgcct ttgaaaaccg aactatacat gtaggttatt
    11941 gccatgactt cactggctac tgcagcagat aattcaacct gggaagtaaa tttcctgtca
    12001 gttatacgat ttaaagcaca gtaccctaat atcgctgtac caaaaagaaa tttaaaaata
    12061 aaatcatatt ccgggcttcc cgtttgatat tcgctcatgg atcgggttag ataatcacca
    12121 agcagcaatg ctgctccaga ggatatcgtt ggccaaatgt attttttgaa actgctccaa
    12181 tccatagagt ctaaagaaaa agtcatggct aaagtgttat aaagttcctt tacacgggta
    12241 gaaagaaaac tccctatgcc ttctacagta cttctcagat aatacgttcg atccggctca
    12301 gtattttgta ccaattgaag cggtaaagct ctgttctctt taggagtagg ttttcgcgat
    12361 tttttttcgg tcgtctttaa tatcttcatg ttataaatcc ttttgtactc caataacact
    12421 ctattggatt tttagagctc aacctgataa ttaacttatc tgtattaaag ttcaagagga
    12481 tttgaaaaat aattacagaa cgattcaaga aaaataagat tctttcagga cttacgaaaa
    12541 actccaaggt cgaggcaaag aatgttttta atgagggagt ttagataaac taaatgaccg
    12601 agttaaaaat ttttttaaca aagagattgg agtttatcgt aagtcctgtc tttaaataag
    12661 gttatctcct gagttgaaac tgacattggc ctaatttacc ctaggttgta cttatccact
    12721 tatgttgcag acgacttaaa gtaccatttt gtataagttc ggccagaata agattgactt
    12781 tatcccgaag tttaaaatta tctttagcaa ccgcgatacc aagtttttca tgtgtttcag
    12841 cctgcaaagg aacagccagg tttgtatagt ttcgaattaa acaggaaata acaggatata
    12901 atttaatgac caaaccaatt ttaccggcag acagatcctg tatggctaat tctatttcat
    12961 tgtatgcata gtatttaata tcactgagta tgttcttacg taataaattc tgagcaactt
    13021 cgtccgaggt attgcctttc tgtataccag ccaccaaacc tttcaaatcc tcaaaagaag
    13081 aaacatttgg ctttaattca gtattaaccg cgataccctg attaaactct aaatagggat
    13141 tcgaaaaaag tacttgtttt gatcggtttg gtgtaatagt cgttcctgaa atgacaacat
    13201 cgtattcttt attagccaag cctaaaaaaa tatcattaaa attttctccc tcatatttga
    13261 tctggactag ctgcaaattt aaaattcgac aaatttcttt tattaaatca ccatcaaaac
    13321 catcacctgg ctcaatatca aacggtggat ctggataagc tgtagccacc aataattttc
    13381 ccgcctttag aatcatagat ataaactctt gttggaatcg atttatttag tatagagtaa
    13441 acagaaaaaa ttcagccaaa tattttttat gccattgatt taaatgaata atttacccat
    13501 gattacacga gtaatatatc caggattggt tatgtaatta acagctaaaa ttataaaaac
    13561 caaaaagaat ataaaaaaat cac
//
LOCUS       DBNCWK010000079        13557 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.079, whole genome shotgun sequence.
ACCESSION   DBNCWK010000079 DBNCWK010000000
VERSION     DBNCWK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13557
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.079"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..188)
                     /locus_tag="AC06IO_001950"
     CDS             complement(<1..188)
                     /locus_tag="AC06IO_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875128.1"
                     /translation="MNHSAKNKMLISVLYCLRHLIALLVMLVGIYLIKLVTVLLYIPS
                     DYSTLSLLSLCRVLWLSN"
     gene            complement(219..1190)
                     /locus_tag="AC06IO_001951"
     CDS             complement(219..1190)
                     /locus_tag="AC06IO_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIO9875129.1"
                     /translation="MSLRILSLSDVKHSITMHQAISALESAFIQLANNTVKLPLRTGV
                     TIEEENGLMLTMPGYLAQEKSLGLKVVSIFPENLTKNKPTITGFIMLLDATTGEAKVL
                     MDAAYLTSLRTGAVSGLATKYFARDNATQVAIIGSGAQAKTQLEAVASVRDIQQVFVW
                     SRNIKNAEKFANEFEDQFDMTICSSVAMAVKDADIICTATNSTEPLIHFGDISPHAHI
                     NAIGSHHPSMKEISHDVLSRAVVIADQLDAVLAESGEIISAVEHNHLKKESIIEIGQW
                     LLQKNTDCKDKLTVFKSVGLAIQDLSVANAVYQNALKKNLGELFSIS"
     gene            complement(1245..1691)
                     /locus_tag="AC06IO_001952"
     CDS             complement(1245..1691)
                     /locus_tag="AC06IO_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIO9875130.1"
                     /translation="MKSIANKENEVAIIHKLIEEYANAARDKDIDKIMSYYAPDIRSF
                     DAVSQLQFKGADDYRKHWQTCLSFCPGPSVFEVHELETLVDNNLAVSYYLTYCGGTNE
                     KGEMQGGWMRGTMVHCKMNGKWKIMHEHYSVPFDMKTGNTLFDLKP"
     gene            2113..3615
                     /locus_tag="AC06IO_001953"
     CDS             2113..3615
                     /locus_tag="AC06IO_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIO9875131.1"
                     /translation="MIKIIREMPHGVAALCLIQAFSTFSYAVQYSSLALFITKQLGIA
                     NLSANSIVGLFLSFSYIFQLIGGVIGSRFISNRLLFVTTILIQNMGLFFLAQANPSLL
                     NIGLSLFMVGSGLNVTSSNSMLTQCFIPQDDRRESAFFLSYAGMNVGYCIGYIACGFF
                     DYSNQYQYLFYASILTNSIALLIIIKNWAYLGDKNFPLPNLTKRNWYFIKNILAIAFI
                     TLLIPFMRVCFEFANFCNGLVIITCLFMFLIIFILGLKQKSSSDRKKMMAYLILAISS
                     VLFWMIYLTGPMGVTLFVKNNVDKHLFSFELATQWIKNINPVVIILGAPIMAMLINKL
                     KAKGFYVSVPVQFSCSFVFLALSFLCLIFGIICSNSYGYISVYWIIGHHILQGMSELL
                     LGPVGYALIGRIAPPKLQGILMGTWMLVTGVAASFSQYFSNTMVQSESVDPLTTNQDF
                     LTAFKQLGLWSLLCAALLYFVSGKLTRIINGIKDNKSVNESKALPIPYSN"
     gene            complement(3620..4201)
                     /locus_tag="AC06IO_001954"
     CDS             complement(3620..4201)
                     /locus_tag="AC06IO_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC4"
                     /protein_id="HIO9875132.1"
                     /translation="MLTITREKFEKIKNQLKEKQLVCNQIAQWKKNPLWLREITHQPE
                     IPPNFLNELSQIRDAIVSFEIDKNTLGLAVFYNELYKLLSPSYNVLTPLDTDLMNSHV
                     LLLQRISNYSGEVDKHIKSLYSDEVKHLDYTEFWSLLNEICSIQEVDRRKHAKEKFSQ
                     SIFLRLYQYNPKIYMDVSARLEEPDEQSHTLDF"
     gene            complement(4566..5102)
                     /gene="cegC4"
                     /locus_tag="AC06IO_001955"
     CDS             complement(4566..5102)
                     /gene="cegC4"
                     /locus_tag="AC06IO_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC4"
                     /protein_id="HIO9875133.1"
                     /translation="MSAKQKELERLLELKKKQEEQQVLNQKDMLERIKLENKYMEFLQ
                     MTSQQMEEELKKRGPVKEVDVKGKDIDPIIADYKKLYSKESWYKEPETKDGKTHLTFP
                     SQEAAGTFFRDQAEKNRSFIVIDAATNKVLAYSNGDGKLYNGNGSLYQGGDFKASKED
                     FTSFKMPEREDPKMGMQL"
     gene            complement(5234..6664)
                     /locus_tag="AC06IO_001956"
     CDS             complement(5234..6664)
                     /locus_tag="AC06IO_001956"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIO9875134.1"
                     /translation="MDLSLLKKINDFEWQIPQHGKMLVPGVIFASEQLILDMDMKVYE
                     QLSNVATLPGIVSAAYAMPDAHWGYGFPIGGVAAFDPDDGGVISAGGVGFDISCGVRL
                     LSTGLKRDELESVKVSLADALFTHIPAGVGSRSGIHLTIKQLDDMMRGGAQWAVKQGY
                     GEAADLERIEDRGCVEGAIPDFVSEPAKKRQKNEMGTLGSGNHYLEVQEVRQIYCSDT
                     ATALNLHEGDIVVSIHCGSRGLGHQIGTDYLRSMVIESQHYGIKLADRELACAPIHSK
                     LGENYLGAMRAGINCALANREIITHFMRNVFQDILPRTQIDLIYDVSHNTCKEEFHEV
                     DGKKKRLFVHRKGATRALGPGHPQLPKAVSHIGQPVIIGGSMGTGSYVLAGVKGSEHK
                     SFSSACHGAGRAMSRHQAMKKWNGKDIVDKLAQQGILIRSGSYRGVAEEAPGAYKDVH
                     SIVDAAEMSGLAKKVAKLIPIICVKG"
     gene            complement(6698..7144)
                     /locus_tag="AC06IO_001957"
     CDS             complement(6698..7144)
                     /locus_tag="AC06IO_001957"
                     /inference="COORDINATES: protein motif:HMM:NF014058.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIO9875135.1"
                     /translation="MISKRQNPLPLNKYWEHFTHEADIGVRGIGPTLTDAFEMGALAL
                     TNVVADASLINPSLSIKIHCEAPNEEILFTDWLNAVIYNMQIHNMLFREFQVKLNRFA
                     LDAVIKGEKVDQKKHQPVVEVKGATYTELKVYKNNNIWIAQCVVDV"
     gene            complement(7111..7395)
                     /locus_tag="AC06IO_001958"
     CDS             complement(7111..7395)
                     /locus_tag="AC06IO_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HIO9875136.1"
                     /translation="MAKFWSQRVTNTSNVLDLEKGVFTWNDPRKIAASLLNSALNSNR
                     RKGTPYQSAMSMLNFYINRAGSKLAAGQKEILMEVKKELRLLAKDKTRYH"
     gene            complement(7479..9032)
                     /locus_tag="AC06IO_001959"
     CDS             complement(7479..9032)
                     /locus_tag="AC06IO_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase AlgX-related
                     protein"
                     /protein_id="HIO9875137.1"
                     /translation="MRIFSRFSDRLVIFAFLVIIFVPGIGMFFEKQADEVRSLLNREP
                     HQLPPINIKKIGRTDFKGIENWFVDHTLFMTSLSKFWSHVVYRLGASIKPGQAILGKE
                     DWLFLGNDYAASIDQYTGRNKPTEEEILLKLSVLKQMNHLAKQNNIPFLVVIAPDKHE
                     IYPEYLPTNVHKSSNKNRLDLLQEGMLARGIDFINLRQKEMEAKNTLGKQYGDLYLKG
                     DSHWNYVGAYVAYQAISDYMQQKGLQSRQLQFHFIPRETTYSDLTNFLQLTHIKSNNP
                     LPDVSNLKIDLFGRDIDGKEIKLDDFQGNPNGVILIAPYENINKAVQNKQTCLLIGDS
                     FSESLSFYFHNDFYNTVRIHSGNTSWNLSDLIQKYHPDLIVYEKVERDLLYPLVNFQT
                     IANQVSLELPKQALAARGELDKFKIGPDTISVNGWAYIPDLDAGNGEVFLKLSMGTHT
                     YLYSMNKMQKQSVNLAFKQDGKHLDLSGFNGTISRKDLPSGLYKVSLIVLNGEEMGET
                     ELPGTYTLS"
     gene            complement(9035..10285)
                     /locus_tag="AC06IO_001960"
     CDS             complement(9035..10285)
                     /locus_tag="AC06IO_001960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIO9875138.1"
                     /translation="MINYLCGLLIEDSKKPGQRKIYLITSVSASLAMLMYFKYFNFFL
                     DSLNSVFDLNVMGISKIVLPLGISFYTFHALSYVVDVYWKKVPAERNFVTFVCYVSFF
                     PQLVAGPIVRYADIRHSFYRRYVTRAGVRNGILRFCFGLAKKVLIANEVAKLADYVFY
                     LPANELTFGVAWLGAIAYALQIYFDFSGYSDMAIGLGLMFGFKYKENFNFPYNALSIQ
                     EFWRKWHISLSSWFRDYLYIPLGGSYKSRFRTQINLIIVFGLCGLWHGANYTFIVWGL
                     FHGFFLILERTHFGYFMKKLPKIIRHGYVLSVVTVGWVFFREDNIQSALVFLKTMFIP
                     AEFNLDSIAACINIILILAFIVSIILALGFNSRVRDSLILSRNKNWIIAYKQSSKVLG
                     ILLLLVSILLLSTNSYNPFLYFRF"
     gene            10672..11310
                     /locus_tag="AC06IO_001961"
     CDS             10672..11310
                     /locus_tag="AC06IO_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875139.1"
                     /translation="MQDSFFSFLSSPINLLFLFVAFFLLFVTPWLWNIVNSAQNIHKL
                     LDPLGIKKKENSLPVTGLLFLPLLLIIIFIEWFWPLANITLFPILASPYIKFNIIAVI
                     SVVILLILIQFDKKKSMGLWVLAYAAVLYLLYLIIIMATVVVGMNAFPASHYIFAPES
                     SMTINRTIIYGLLIAHLIFIWLTISLKFTWRLLLMILYTLTLSFAYFSLIVS"
     gene            11537..12637
                     /gene="wipC"
                     /locus_tag="AC06IO_001962"
     CDS             11537..12637
                     /gene="wipC"
                     /locus_tag="AC06IO_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipC"
                     /protein_id="HIO9875140.1"
                     /translation="MNRMALFIIFIIHCYFSQSFAEQEKPYNELYVKQANLKQYPKEI
                     NSYPPGVEITIGDLHGNALKLLYFLIRNDVIKIDKEDYKLFVTIYQKNPNELTTKDLS
                     FFQIIVNSAEINTQHKIRFLGDDLCDRGMNDYYTLVIYKKLDQANVPFEVILSNHGNF
                     FLTAYERPEQSFNYNPYGEGENESTVQSMLNMGRLIDRGLIDKQDILEMIQYHYLKHI
                     VLPGYTHNKDKNELTIYTHAPIDLGIISALANDLQVPFKDSNLHELTKSLDSINSKIK
                     QWILSNTFTRHYKELNEAHNQTNTPSPIKQILWNRDYSILDRHANPNNKPYGINYVHG
                     HDSMPNVFDLDNLFGKGEDFYQGPYAVHITHS"
     gene            complement(12686..>13557)
                     /locus_tag="AC06IO_001963"
     CDS             complement(12686..>13557)
                     /locus_tag="AC06IO_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HIO9875141.1"
                     /translation="KSGTLLTDKFSNKFDSKASSIGVYKTEQVYYGREGLSLRLVGLD
                     TKFNDNAFNRYIVMHGGWYMDEQFIKRYGRPGRSWGCPALPLPIKKQIIDTIKDNSLL
                     VVYYPSDEWFNKSKFLNCSKQKSDQVAANRLSETQTPVDDEIREDILFVDLNKNNSRE
                     EHEPIITMSADAYERIFHSQPPLSRMLRRQINNAEYIALSKEEFNKLVLQGNREGLGE
                     IHFVIPVIIMEHGYYETQMQIVNMGKIKEVQPNSDTSRITQEPAKSYRIDFESKPALN
                     LKTTNRFIRWLGL"
BASE COUNT         4182 a         2783 c         2261 g         4331 t
ORIGIN      
        1 tcattggaca accacaagac gcggcacaaa gacaaaagtg aaagcgttga ataatcagaa
       61 ggaatataca atagtactgt tactagctta atcaaataaa taccaactaa cataaccagt
      121 aaagctatca gatgacgtaa gcaatataaa accgaaataa gcattttatt ttttgcagaa
      181 tgattcattt acactcactc taatcggttt tacccttact aacttataga aaataactca
      241 cccagatttt tcttcaatgc attttgataa acagcgttgg caacacttaa atcctgtatt
      301 gccaaaccaa cggatttaaa taccgtgagc ttatctttgc agtcagtatt cttttgtaat
      361 aaccattggc cgatttcaat aatggattcc ttttttaaat gattgtgctc tactgcacta
      421 atgatttcgc ctgattcagc taaaacagcg tctaactgat cagcaatcac cactgctcgg
      481 cttaacacat catggctaat ttctttcatt gaagggtgat gagagcctat ggcattaata
      541 tgggcatgtg gcgaaatgtc tccaaaatga atcagtggtt ctgtgctatt ggttgctgta
      601 caaataatat cggcatcttt cactgccatg gcgacagaag aacaaattgt catatcaaat
      661 tgatcttcga attcgttagc gaatttttcg gcatttttaa tattgcgcga ccaaacaaaa
      721 acctgttgta tatctcgcac agaagcaact gcctcaagtt gtgttttcgc ttgagcacct
      781 gacccaatga ttgcgacctg agtcgcatta tcgcgcgcaa aatattttgt cgctagtcct
      841 gaaacagcac ctgttcttaa ggaggtgagg taagcagcat ccattaatac tttagcttca
      901 cctgttgttg cgtcaagcaa cataataaaa ccagtgatag taggtttatt tttagtcaaa
      961 ttttcaggaa aaatggatac tacttttaag cccaagcttt tttcttgggc aagataaccc
     1021 ggcatagtca acattaatcc gttttcttct tcaatggtaa cacccgttct caagggcaac
     1081 ttcaccgtgt tatttgccaa ttgaataaat gcgctttcca aagcactgat tgcctgatgc
     1141 atggtgatgc tatgctttac atcgcttaaa gacaatatcc tgagactcat atcaataaac
     1201 cctatacttt agttaactac gtagtgactg aatagaatca catcttatgg ttttaaatca
     1261 aataaagtat ttcctgtttt catatcaaag ggaacagaat aatgctcatg catgattttc
     1321 cattttccat tcattttgca gtgtaccatt gtacctcgca tccaccctcc ttgcatttca
     1381 cctttttcgt tcgttccacc acaataagtc aaataatagg aaaccgccag attattatca
     1441 accaaagttt ccaactcatg tacttcaaat actgaaggtc ccggacagaa tgaaagacag
     1501 gtttgccaat gttttctata atcatcagct cccttaaact gaagctgtga aactgcatca
     1561 aaagaacgaa tatctggtgc atagtaggac atgattttat caatgtcttt atcgcgtgct
     1621 gcattggcat actcttcaat gagcttatga attattgcca cctcattttc cttattcgca
     1681 atagatttca tctttatctg ctttaattgg cttcttcctg aattttaatt gtagcaaatt
     1741 ctgcaaaaaa cctaatgaat tattaacctg caacagatgt tgctaaagca acattttttt
     1801 gctttgttaa aaaagaaccc caaatagagt tactctgttc cctgaggttt tttgataccg
     1861 atgcagcgaa tggattacct catcatatag agaaatctga ctctcaacag aaaaaatatt
     1921 ctgcggaatc atttaaataa aataatctat acaattcaaa cgggtttgat aaacgaagct
     1981 taatcaacgt ttctttccta attttactaa agtaaagttt ggaaagaaat tgaatggcaa
     2041 gttgttacat tcttgaaggc aactgattat tgatggtaaa gtctattctc ataaaatcag
     2101 ccaaaatgtt tgatgattaa aataattcgg gaaatgccgc atggagttgc tgcgctctgc
     2161 ttaattcagg cattttcgac tttttcatac gctgttcaat attcttcgct cgcacttttt
     2221 attaccaaac aacttggaat tgccaattta tcagccaata gtatagtggg cctgttttta
     2281 agttttagct atatttttca actaattggc ggtgtcattg gaagccgttt tataagtaac
     2341 agactactat ttgttacaac tattcttatt caaaatatgg gtttattctt tttagcgcaa
     2401 gcaaatcctt ccttgcttaa cataggctta agtttgttta tggtaggttc tggtttgaat
     2461 gttacttcca gcaacagcat gcttactcaa tgtttcatac cacaggatga tcgcagggaa
     2521 agcgcatttt ttctcagtta tgccggcatg aatgtcggtt attgcatagg ttatatagcc
     2581 tgcggttttt ttgattactc caaccagtat caatatttgt tctatgcaag catcctgact
     2641 aactccattg ctttattgat tattatcaaa aactgggcct accttggcga taaaaatttt
     2701 ccattaccca atctcactaa acgaaactgg tatttcataa aaaatattct ggcaatagca
     2761 ttcattacac tccttattcc ttttatgcgg gtatgttttg agtttgcaaa tttttgtaat
     2821 ggattggtaa taattacctg tttattcatg tttcttatca tctttatcct tgggctaaaa
     2881 caaaaatcct cctccgacag gaaaaaaatg atggcctatt taattttggc aatcagttcg
     2941 gttttatttt ggatgattta tctaactggc ccgatgggtg taactctttt tgtcaaaaat
     3001 aatgtggata agcatctttt cagctttgaa ttggctactc aatggataaa aaatattaat
     3061 cccgtggtca ttattttagg agcaccaatc atggccatgc tcattaacaa attaaaagca
     3121 aaaggttttt atgtctctgt tcctgtgcaa ttttcctgtt ccttcgtgtt tttagccctg
     3181 tcctttttat gccttatctt tggaattatt tgttcaaatt cctatgggta tatcagcgta
     3241 tactggatca taggccatca tatcttgcaa ggaatgtctg agttgcttct tggccctgtt
     3301 ggctatgcgt taattgggag aatagctccc ccaaaactac aagggatttt aatgggcacc
     3361 tggatgctgg ttacaggggt cgcagcctct ttttcccagt atttctccaa taccatggtg
     3421 caatcagaat ctgtagaccc cctaactaca aatcaggatt ttctaactgc atttaagcaa
     3481 ttggggcttt ggtctttact ctgtgctgcc ttgttatatt ttgtttcagg taaattaacc
     3541 aggataataa atggtataaa agacaataaa tcagttaatg agagcaaggc tctgcccata
     3601 ccttactcta actaatcatt taaaaatcca aagtatggct ttgctcatcg ggttcttcca
     3661 aacgagcaga aacatccatg taaatttttg gattgtactg atacaaacgt aagaatatac
     3721 tttgggaaaa tttttccttt gcatgtttgc gcctgtccac ctcttgaata gaacatatct
     3781 cattaagcag cgaccaaaac tcagtgtaat ccaaatgctt cacttcatct gaatataaac
     3841 tttttatatg cttgtctacc tcgccggagt agttgctaat tctctggagc aatagcacat
     3901 gactattcat aagatctgta tcaagtggtg ttaatacatt gtacgatgga gataaaagct
     3961 tatacaattc attataaaat acagccaaac ctaaggtatt cttatcaatc tcaaaggata
     4021 caatagcatc ccgaatctgg cttaattcgt ttaaaaaatt ggggggaatc tctggctgat
     4081 gagtgatttc tctcaaccac agcggatttt ttttccattg tgcaatttga ttgcaaacta
     4141 attgtttctc ttttagctgg tttttaattt tctcaaattt ttctctggta atagttaaca
     4201 tgtttttttc tcttataaat aagcaagcga tactcaagaa gttaggtttg cttccaaaat
     4261 tgggaatagg cttcttacat agcctgtctc tttcatttta ttgctgtgtt gcaaacactt
     4321 ctgcagtgct catttactgc atgtaccccc ctgcccctca aaatgtttgc gccttgccct
     4381 taaacaaaat aatcagctta tgcaagaagt ttaatatttg ataacggtta tgttttttca
     4441 gactatatga tacattattt gacacttact gtccaaattg gacagtaatg tttttttgga
     4501 agagaacgag aatgaataat tataaaaaga ttacctgcat cgaagccaat ttaaatcaaa
     4561 gcagattaaa gttgcatccc cattttagga tcttctcttt caggcatttt aaagctggtg
     4621 aaatcttctt ttgacgcttt gaagtcaccc ccttgataaa ggctaccatt cccattgtat
     4681 agttttccat cgccattaga ataggcaagc actttattgg ttgcagcgtc aataacaata
     4741 aaagatctgt ttttttcagc ctgatctcta aagaaggtac ctgctgcttc ctgagacggg
     4801 aaagtcaaat gagtttttcc gtcttttgtt tcaggttctt tataccaact ttccttacta
     4861 taaagctttt tgtagtctgc aataatggga tcaatatctt tacccttcac atcaacttct
     4921 tttacaggac ctcttttctt taattcttct tccatttgct ggctggtcat ctgcaaaaat
     4981 tccatgtatt tattctcgag ctttattcgc tccagcatat ccttctgatt taaaacttgc
     5041 tgttcttctt gttttttctt taattcgagt aatctctcaa gctctttttg ttttgcactc
     5101 attatagata tctcctcgta ctattgagct aattttagtc taaaagctta tcgaaaaccg
     5161 ctcttagttt attttattta cagattcctt acaattataa gctgctccat tcacctcaat
     5221 aaacaatgga ttgttaacct ttgacacaaa tgatgggaat taatttagca actttttttg
     5281 ccaaacccga catttcagcg gcatcaacaa tagaatgaac atctttataa gcccccggag
     5341 cttcctcagc aaccccacgg tatgatccgc tacgaattaa aattccttgt tgagccaatt
     5401 tatctactat gtctttcccg ttccatttct tcattgcctg atgacgactc atagcgcgac
     5461 ctgctccatg acatgccgag ctaaaagatt tatgctcaga acctttgacg cctgctaata
     5521 cataggaacc tgttcccatg ctccctccaa taatcacagg ctggcctata tgagaaacag
     5581 ctttaggtag ttgtggatgg cctgggccca aggcacgagt tgctcctttt cgatgaacaa
     5641 ataagcgctt tttcttgcca tctacctcat gaaattcttc tttacaagta ttgtgagaaa
     5701 cgtcatagat tagatcaatc tgagtgcggg gtaaaatatc ctgaaacaca ttgcgcataa
     5761 aatgagtgat gatttctctg ttggctaaag cacaattaat tcctgctctc atagcgccca
     5821 ggtaattttc ccctagcttt gaatgaatag gagcacaagc caactcccta tccgccagct
     5881 tgataccata atgttgtgat tcaataacca tggaacggag gtaatctgta ccaatttgat
     5941 gccccaatcc tctggaacca caatggatac tcactacaat atctccttca tgcaaattta
     6001 aagctgttgc tgtatcggaa cagtagattt gcctgacttc ctgcacttcc agataatgat
     6061 tacctgaacc caaagtaccc atttcatttt tctggcgctt tttagctggc tcagagacaa
     6121 aatcagggat agctccttcc acacaaccac gatcctcgat acgctctaaa tcagcagctt
     6181 caccatagcc ttgttttact gcccactgtg caccccctcg catcatatca tcgagctgtt
     6241 tgatagttaa atgaatgccg cttctactac ctactccagc tggaatatga gtaaataaag
     6301 cgtctgcaag gctaactttg acagactcca attcatctcg cttcaatcca gtagataaga
     6361 gcctcactcc gcatgaaata tcaaaaccca cgccacctgc tgaaataacc cctccatcat
     6421 ccggatcaaa agcagcgaca ccgccaatcg gaaaaccata cccccaatgc gcatcaggca
     6481 tagcataagc agcagaaaca attcccggca aagtagcaac attagataac tgttcgtaaa
     6541 ccttcatatc catatcaaga atcagctgtt ctgaagcgaa aataactcct ggcaccaaca
     6601 tctttccatg ttgaggtatt tgccattcaa aatcgtttat ttttttcaag aggcttaaat
     6661 ccatcactgt tcaccacatt aattcctgtt aaggagttta tacatcaaca acacactgag
     6721 ctatccaaat attattattt ttgtatactt ttaattcagt ataggtggca cctttgacct
     6781 cgacaactgg ttgatgtttt ttttgatcaa ccttttcacc cttaatgaca gcatccaaag
     6841 cgaatcgatt gagttttact tgaaactcac gaaacagcat attatgaatt tgcatattat
     6901 aaatcacggc atttagccaa tcggtaaata aaatttcttc attgggtgct tcacaatgta
     6961 tttttattga tagagaaggg ttgattaggc ttgcatcagc aactacattc gttaatgcca
     7021 aagcccccat ttcaaaggca tctgtgagtg taggtccgat gccgcgaact ccaatatctg
     7081 cctcgtgagt gaaatgttcc cagtatttat ttaatggtaa cgggttttgt cttttgctaa
     7141 taatcgcaac tcctttttaa cttccattaa aatttctttt tgcccggcgg ctaatttgct
     7201 tccagctcga ttaatgtaaa aattcaacat agacatcgct gattgataag gtgtaccttt
     7261 gcgcctattg ctatttaatg ccgagttaag caaagaagca gcaatttttc gggggtcatt
     7321 ccaggtaaat accccttttt cgagatcaag cacattgctt gtattcgtca ctctttgtga
     7381 ccaaaacttt gccattgatt cgaccaactg aatctcatac actcatatta tagcgattta
     7441 atataagccc tgctgttttg agtcagggca aatgccatct aacttaaagt ataagtgcct
     7501 ggaagttcgg tttcgcccat ctcctcgccg tttaagacaa tgagtgatac tttatataga
     7561 ccagaaggca aatcctttcg cgagatagtt ccgttaaacc ctgacaaatc aagatgctta
     7621 ccatcctgtt tgaaagccag attaacactt tgtttttgca ttttattcat gctgtataaa
     7681 taagtatgag ttcccatcga caactttaaa aacacttctc catttccagc atctaaatcg
     7741 ggaatataag cccagccatt gactgagatt gtatctgggc caatcttgaa tttatcgagc
     7801 tcccccctgg cagctaaagc ctgtttagga agctcaaggc ttacttgatt tgctatagtt
     7861 tgaaaattga cgagaggata gagcaaatca cgttcgactt tttcatacac aatcaaatca
     7921 ggatggtatt tttgaattaa atcagacaaa ttccaggagg tattcccgga gtgaatacgt
     7981 actgtattat aaaaatcatt atgaaaataa aaactgagag actccgaaaa gctgtctcct
     8041 attaataaac aggtttgttt gttttgtaca gctttattaa tattctcgta aggcgcaatg
     8101 aggattacac catttggatt gccctggaaa tcatccaatt taatctcttt tccatcaatg
     8161 tctctgccaa acaaatcgat ttttaaattt gaaacatctg gaagaggatt attggatttg
     8221 atatgcgtca actgcagaaa attggtaaga tcagagtagg tggtttctct gggtatgaaa
     8281 tgaaactgta attgtctgct ctgtaaccct ttttgctgca tgtaatctga tatcgcctgg
     8341 taagcaacat aagcaccaac ataattccag tgactatccc cctttaaata aagatctcca
     8401 tactgtttgc caagagtatt tttggcttcc atttcctttt gtctcaaatt gataaaatca
     8461 atacctcgag caagcattcc ctcttgtaaa aggtcaagcc tgttcttatt ggagctttta
     8521 tgaacgttag tcggtaaata ttccggataa atctcatgtt tatctggggc aataaccacc
     8581 agaaaaggaa tgttattttg tttagccaga tgattcatct gttttaagac agataatttc
     8641 aagagaattt cttcctcggt aggcttattt ctcccggtgt actgatcgat agatgccgcg
     8701 taatcattgc ccaaaaacag ccaatcctct ttccctaaaa tggcttgtcc aggtttgatg
     8761 ctggctccca accgatatac gacatgactc cagaatttac tcaacgaggt catgaacaaa
     8821 gtatgatcaa caaaccaatt ttctatgccc ttgaaatcag tacgacctat tttttttata
     8881 ttaatggggg ggagttgatg aggttccctg tttaatagag atctcacttc atccgcttgt
     8941 ttttcaaaaa acataccaat ccctggtaca aaaatgatta ccagaaaagc aaagatgacc
     9001 agcctgtcag aaaacctgga aaaaatacgc atcatcaaaa cctaaaatag aggaaagggt
     9061 tataagaatt agtgcttaat aataatattg aaaccagtaa aagcagtatt cccaaaacct
     9121 tgctggattg cttgtatgcg ataatccagt ttttatttct acttaagatg agactatccc
     9181 ttaccctgct gttaaaccct aacgcaagta taatgctcac aataaaagcc aaaataagaa
     9241 taatattaat acatgctgct atggaatcaa gattgaactc agcaggaata aacatcgttt
     9301 taaggaatac cagtgccgac tgaatattat cttctctgaa aaatacccat ccgactgtca
     9361 caacagagag aacatagcca tggcgaataa ttttaggtaa ttttttcata aaatagccaa
     9421 agtgggttct ttccagaata aggaaaaacc cgtgaaataa cccccaaaca ataaaggtat
     9481 aattagcgcc atgccataaa ccgcacaaac cgaatacaat gatcaagtta atctgcgttc
     9541 gaaatcttga cttataactc cctccgagcg gtatatataa ataatctcga aaccagcttg
     9601 ataaggagat atgccacttc cgccagaact cctgtatcga taaggcatta tagggaaaat
     9661 taaaattttc cttgtattta aacccaaaca tcaaaccaag gcctattgcc atatcagaat
     9721 agccggaaaa atcaaagtag atttggagag cataggctat agctccgagc caggccaccc
     9781 caaaagtcaa ctcattggca ggcaaataaa atacataatc agcaagcttt gccacttcat
     9841 tggcgataag aacctttttt gccaagccga aacaaaaacg cagaattcca ttgcgcactc
     9901 ccgcacgagt tacatatctc cgatagaatg aatgtcttat atctgcatag cgaacgattg
     9961 gtcctgcaac caattggggg aaaaaagaca cataacatac gaaagtaaca aaattacgtt
    10021 ctgctggtac ttttttccaa tacacatcaa ccacataact taaagcatga aaagtataaa
    10081 aacttattcc aagaggcagc acaattttac tgattcccat gacatttaag tcaaagacac
    10141 tgttcagtga atcaagaaaa aaattaaaat acttaaaata catgagcatg gccagtgatg
    10201 cgctcacaga ggtgattaaa tagatttttc gttgtcctgg ttttttgctg tcctcaatca
    10261 ataggccaca tagataattt atcaaaatgc acgccagcat aataaagata tgatctttct
    10321 caccccaata ataaaaggac aagctaaaga gcattaaaac aaaattttgc cacttgatac
    10381 ttttaaaaca gactagataa gtcaggttaa ggcacggaag aaataaaaaa agaaatatta
    10441 aagatgaaaa aaccattcta tctccgtgaa ggcatttgcg gaattaatta aacttgtatt
    10501 aactgttcaa gacgttagat tcatcgcgtg aagcgcatta tagggaagtg ttatccgttg
    10561 tgcaaataga atacgataaa aagaaccatc tgcaaaatct gaattgaagc tgccattata
    10621 acattcatta gctatactta acctaaggca gcattcgttg atgaattaat aatgcaagat
    10681 tcctttttct catttttatc aagccctatc aatttactct ttctttttgt tgcatttttt
    10741 ttgctttttg taacaccatg gctctggaac atagtgaact cggctcagaa cattcacaag
    10801 cttcttgacc cgcttgggat taagaaaaaa gaaaattcac tgccagtgac aggactgtta
    10861 tttctcccgc tgctcctgat aataattttc attgaatggt tttggccttt ggcaaatatc
    10921 acgttgtttc cgatactggc ctcaccctat attaaattta atattattgc agtcatttca
    10981 gtagttattc ttttgatttt aatccaattt gataaaaaaa aatcaatggg gctttgggtg
    11041 cttgcctatg cagctgtact ttatttattg tatttgatta tcatcatggc gacagtagtg
    11101 gtagggatga atgcttttcc tgcaagccat tatatttttg cccctgaaag cagcatgaca
    11161 atcaatagga caataattta tggtttatta atagcccatt taatttttat ctggctaacc
    11221 atatcgctta aattcacatg gcgtttattg ttgatgatac tctatactct gaccctgtct
    11281 tttgcttatt tctctcttat agtctcttaa ataagctgtt tctttcattt tattgtagtg
    11341 ttgcaaacgt ttgtgtggtg cagaaactcc gcccctcaaa acgttttcac tttaccctgg
    11401 caggacttac gaaaaacccc aaggtcgagg caagtggtct accattgctt tcatgaacaa
    11461 accaaggtat acctagctta ccagattacg gtcatcgaca cagaacaata aaaaattttt
    11521 acaggatgta gcattcatga accgcatggc acttttcatc atttttatta ttcattgtta
    11581 tttttctcaa tcatttgcgg aacaggaaaa accatacaac gaactgtacg tcaagcaagc
    11641 aaacctgaaa cagtatccca aagaaatcaa tagttatcca cctggtgtag aaatcactat
    11701 tggagatttg catggaaatg cattgaaatt gctctatttt ttaattcgca atgatgtgat
    11761 taaaatcgac aaagaggact ataagttatt tgtaactatc taccagaaaa atcctaacga
    11821 actgacaaca aaagatttgt cttttttcca aattatagtc aattccgctg aaatcaatac
    11881 ccaacacaag atacgttttc tgggagatga tctatgtgat cgtggcatga atgactatta
    11941 cactttggtt atttataaaa agctcgacca ggccaatgtc ccttttgagg tgattctatc
    12001 caatcatggc aacttttttt taacagccta tgaaagacct gagcaatctt ttaactataa
    12061 tccttacggt gaaggtgaaa acgaatccac agtgcaatcc atgctaaata tgggaagact
    12121 gattgatcgg ggattgattg ataaacagga cattttggaa atgattcaat atcattactt
    12181 aaagcatata gtacttcccg gttatacgca taataaagat aaaaatgagc ttaccattta
    12241 tactcatgcg ccaatcgatt taggaataat ctcagcatta gccaatgatc tgcaagttcc
    12301 ttttaaagac tctaacttgc atgaattaac caaaagtctt gactcaatta actccaaaat
    12361 aaaacaatgg atactctcta acacgttcac tagacattac aaggagctta acgaagcaca
    12421 caatcagacc aacacgccaa gcccaattaa gcaaatattg tggaatcgtg attattctat
    12481 cctcgatcgc cacgctaatc ccaataacaa accctatggc attaattatg ttcatggcca
    12541 tgactctatg cccaatgtgt ttgatctgga taacttattt ggaaaaggcg aggattttta
    12601 ccaaggacct tacgctgtgc atattacaca cagctagctg tgaataaact caaccctgtc
    12661 attaaaagag gggaacaaat tgaaattata gccccagcca ccgaataaag cggtttgttg
    12721 tttttaaatt gagggctggt tttgattcaa aatctattct atagctttta gcaggttcct
    12781 gtgtaatacg agacgtatca gaattgggtt gaacttcctt aattttaccc atattgacga
    12841 tttgcatttg agtttcataa tacccatgtt ccataataat gacaggtatt acaaaatgaa
    12901 tttctcccaa accctctcta ttcccctgaa gaaccagctt attaaattct tccttactca
    12961 aagcaatgta ttctgcattg ttaatttggc gccttaacat gcgggacaaa ggaggttgag
    13021 aatgaaaaat acgctcataa gcatctgctg acatagtaat gataggttca tgctcttcac
    13081 ggctgttatt tttattgagg tcaacaaaca aaatatcttc ccgtatttca tcgtcaacag
    13141 gagtttgggt ctcagaaagc ctgttagcag cgacttgatc agatttctgt ttactgcaat
    13201 ttaaaaattt cgacttatta aaccactcat cgctaggata ataaacaacc agtaaagaat
    13261 tatcttttat agtatcaata atttgttttt taattggcaa aggtaacgca ggacagcccc
    13321 aactacggcc aggccgacca tatcttttga tgaattgttc atccatatac catccgccat
    13381 gcatgacaat atagcgatta aaggcattat cattaaactt ggtatccaaa cccactaatc
    13441 gtaaagacaa gccttctcga ccataataaa cttgctctgt tttataaaca ccaatactgc
    13501 tggccttact atcaaattta tttgaaaatt tatccgttaa aagagtccct gatttta
//
LOCUS       DBNCWK010000080        13404 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.080, whole genome shotgun sequence.
ACCESSION   DBNCWK010000080 DBNCWK010000000
VERSION     DBNCWK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.080"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..2311)
                     /locus_tag="AC06IO_001964"
     CDS             complement(<1..2311)
                     /locus_tag="AC06IO_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HIO9875142.1"
                     /translation="MKEKMILDKSNIANAIFRNLSADEIRRVKSVSHIFYERATEYLI
                     GNLKNESFHLSRVVPPSDAIQREQKIEFCAKSLQALICSISKGEEGKSENLHKFNNMM
                     EKIRRAVQSNLGLSRVQRETYETFHPIYVKLGSSVFPINDEGQLDPGSIVYRTAPVKG
                     SDERKSSWVILLKDDSKEDGLSICFDTKFRKYLLHIDGSGHVISQLTLRWTAIYDDLL
                     PDKAAQQPLRSFTPWQRHAEANFFNGRSTNKILGLLTKNYPEIFNYDPTSYFREEPVF
                     SIELNRNAPKKLSTDKKNIYLTLDESFFKTPNGFETGLNRQPILAVIKTNSPLRGDCF
                     SVVPAIYEKQQLHILIKGHQQQTQVLDVFHANFKTGELLSWELLDDVLIFGEPSTEYK
                     PALTDLNLLSNQQSELMSHVSTAPMVRGSGPGARQYWLNGFFKSTVYSEMIVIAEIFN
                     SRFMSTPLASLEQTLHHSDATKSFYRLQFSRQELEKAKEFVFQLAIPNEFSKQEDFDF
                     FYGENGNKLEEITKLAILMLKRMVPKEFHHKIRIGINMCCPAASVENIGSGFGLRNKP
                     DFIERIVKAIKDTDSNLKVEFKTLMLSHEDSYGDSNQTSGSTKNISEVQNERLTRIVS
                     KTKADRLVYQGKPRNIEAYSGDAVSSIIREAKTPKEFQFGYSGMVATITNHDIHQLGY
                     KVVGEPHYSVQSILEKIKKQLGEVPIEFEVMLGRVLLGSAWVLQGRYASDAEILLHTL
                     LQAAIEQEWAEGMGRYGVDIYQIQILYNIR"
     gene            complement(2693..3451)
                     /locus_tag="AC06IO_001965"
     CDS             complement(2693..3451)
                     /locus_tag="AC06IO_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIO9875143.1"
                     /translation="MPNSSNVLRHMENHLALTTCEDIKNIMQPLLAKHGMTVFNYYRI
                     YFDSTVIRLSTDQKWTEHYFKMNYLETLTIPESYLKKTLNYYLWLTDDCPLMLQDAAI
                     NFDMANGISIAKINHDSIEYFCFASTRDNTSIVNNFYLNNLDVLEQYSLYFKDQFNSI
                     ISQFEKNKIILPYNATCCQSNKINKEIKRSLLSPRQKDCAKLLLQGMSYKEIGKILQL
                     SARTVETHVNQLKTKLGCDNKAELILQLNGIITR"
     gene            complement(3713..4459)
                     /gene="rluB"
                     /locus_tag="AC06IO_001966"
     CDS             complement(3713..4459)
                     /gene="rluB"
                     /locus_tag="AC06IO_001966"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HIO9875144.1"
                     /translation="MSSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAG
                     PSDKISVKGKLIPNPLKGKQNIRVLLYHKPVGEVSSRHDPKFEKTVFDNLPHLRQGRW
                     VQVGRLDLNTSGLLIFTNNGELANQLMHPKYGLEREYAVRVHGQVSQEALNNLLKGVM
                     LDDGLAKFTKIQFRGGEGTNSWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLA
                     MPRFLSRGQFYELTPKEVTEFLDSLPNELS"
     gene            complement(4449..5030)
                     /gene="scpB"
                     /locus_tag="AC06IO_001967"
     CDS             complement(4449..5030)
                     /gene="scpB"
                     /locus_tag="AC06IO_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIO9875145.1"
                     /translation="MIDNELKNIVEALVLSSNEPVTLEKIQETFDEWQRPTLEDLQAI
                     IESLKADYSSRAFELVQVASGFKVQTKSKYAAWVARLQIEKPAKYSRALLETLAIIAY
                     KQPVTRADIEDIRGVAVNSQIMKTLMEREWIRIAGYKDVVGKPAVYTTTKEFLNYFNL
                     NYLSELPPLPEIMDTLTLHEATQSIEECTQNEQ"
     gene            complement(5032..5823)
                     /locus_tag="AC06IO_001968"
     CDS             complement(5032..5823)
                     /locus_tag="AC06IO_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIO9875146.1"
                     /translation="MNIDLQPAPVVKAVVDGKEITQFPEDLYIPPDALEVLLDSFTGP
                     LDLLLYLIRKQNIDILDIPMTSITNQYLQYIQLMENRRMELAAEYLLMAAMLAEIKSR
                     LLLPVTPDSEEEEEDPRMTLVRQLQAYEQIKQAAELLDGLPRQDRDNFTIQVNPDEVE
                     TIKVYPEVELSELVEAMKVLLKREEHYSKHQVSRELMSVRERMSRILFQLQHQKILEF
                     SQLYTLEEGRMGLVVSFLAILELAKQSLLIITQTQPFSPIHLQAA"
     gene            complement(5823..7037)
                     /locus_tag="AC06IO_001969"
     CDS             complement(5823..7037)
                     /locus_tag="AC06IO_001969"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIO9875147.1"
                     /translation="MSALFTSNKRVVSGMRVSGRLHLGHYHGVLKNWIKLQHQYDCFF
                     FAADWHGLTTQYEDPNFIEKHLWDMIIDWLACGVNPGLCKIFIQSWVPEHAELHLLLS
                     MITPLGWLERVPTYKDQQEKLKEKDLSTYGFLGYPLLQSADVLLYHADYVPVGEDQVA
                     HIELTREIARRFNYLYGREANFEELAAAAIKKMGKKNGKIYTELRRQFQEHGIHESIK
                     TAQALLEDQPNLSIGDKERLLGYLEGSGKIILSEPQPLLTETAKMPGLDGQKMSKSYH
                     NTIMLRDEPALVEKKILTMPTDPARVKRTDPGEPEKCPVWQFHKVYSNEEVKDWVQKG
                     CRTAGIGCVDCKRPVIGAIQQELKPIQEAISDYEADLGSVKRIVAEGSEAAREEGNKT
                     LNTVREVMGLDY"
     gene            complement(7283..7903)
                     /locus_tag="AC06IO_001970"
     CDS             complement(7283..7903)
                     /locus_tag="AC06IO_001970"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIO9875148.1"
                     /translation="MSQFFALHPDNPQARLLRKAVSIIEEGGLIVYPTDSGYALGCSL
                     GNKSALERIRRLRQLDKHHNMTLVCRDLSQLGTYAKVSNPIFRLLKAFTPGSYTFILN
                     ATHEVPRLMLHPKRRTLGLRVPDNTITLSLLECLEAPLMSTTLILPGAEAPLSEPEAI
                     KDLLGNQIDLIIDGGNCGHEPTTVVDLTGDYPIIIREGKGDPEPFK"
     gene            complement(7900..8739)
                     /locus_tag="AC06IO_001971"
     CDS             complement(7900..8739)
                     /locus_tag="AC06IO_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIO9875149.1"
                     /translation="MIDLHCHSSFSDGALTPEELIKRATTLQVQCLSLTDHDTVAGYD
                     ALYDAAIHTNIKIVKGIELSTRWKKYDIHILGYQINHTAELQELIAHQNKSRIERAEK
                     IGESLHSVGIDNAYEKACLLAGHKRVGRPHFAQVLINEGKAKDMKSAFKNYLGRGKCA
                     YIPTLWISMHDAVKGISHSGGQAVIAHPLKYGLTRSKLRELITEFKEAGGVGMEVVSG
                     EMTTVQIQEMAGMCERFHLLASSGSDYHSDAQSCINLGRQRQLPIICTPIWQEWNMKQ
                     GTL"
     gene            9162..9968
                     /locus_tag="AC06IO_001972"
     CDS             9162..9968
                     /locus_tag="AC06IO_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIO9875150.1"
                     /translation="MIICGQSGDHFEEIKKVLKLLYPLTSEPIDTKTLRVLINEDYQT
                     AMTHHIRLSRKSISARNSKQAAYLDSINTHDITFAVGPAGTGKTYLAVSKAIECFEKG
                     EVQRLIFVRPAVEAGEKLGFLPGDLVEKVLPYLRPIYDALYEMIGFKETQKLIQTDII
                     EILPLAFMRGRTLNESFIILDEAQNTTIMQMKMFLTRMGFGSKTVITGDITQVDLPKG
                     IDSGLAHAIQLFRHFPDISIHTFTSREVVRHPLVSKIVDCYDNEMERIKK"
     gene            9965..10441
                     /gene="ybeY"
                     /locus_tag="AC06IO_001973"
     CDS             9965..10441
                     /gene="ybeY"
                     /locus_tag="AC06IO_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIO9875151.1"
                     /translation="MTYHIDIQNATGKLLPLSEDEITKLASLALRDHKQDAELTVRLV
                     DVEEMTYLNHTYRKKNKPTNVLAFPCSLPENIELECPLLGDVVICPEVLLAESAQFNK
                     SLHAHWSLILIHGVLHLLGYDHIKDEEASIMQMLEAKLLAELGYANPYEVEENELE"
     gene            10401..11291
                     /locus_tag="AC06IO_001974"
     CDS             10401..11291
                     /locus_tag="AC06IO_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIO9875152.1"
                     /translation="MLIHMKSRKMNLNKLEDGGSWFSRLKQFLQGEPQNQEELVSLLR
                     DAQIRSLINSETLAMIEGAILFSKMRVRDIMLPKNQMVCINKDDDFKHIISIVTQTGH
                     SRFPVTGENSDEVVGILHAKDLLKYQPENMESFDLLDICRQVTFVPESRRLDSLLSEF
                     RSNRNHMAIVVDEYGEVSGFVTIEDIIEQIIGDIEDEFDIDEDAYIKAHEGHCYIIKA
                     HTPIEEFNEQLNADFSDETYDTIGGIVLNKFGYLPQRGEVIIIDDFEFKVLNADSRRI
                     KLLECLDKRSPEKLNSTVEG"
     gene            11299..11979
                     /gene="cpxR"
                     /locus_tag="AC06IO_001975"
     CDS             11299..11979
                     /gene="cpxR"
                     /locus_tag="AC06IO_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CpxR"
                     /protein_id="HIO9875153.1"
                     /translation="MSSSILIIDDDTELTDLLTQYLEPEGFNVVCVHDGENGVKRALN
                     QVFDAIILDVMLPKLNGFEVLKAIREHLETPVLMLTARGDDIDRIVGLEIGADDYLPK
                     PCNPRELVARLRAILRRTQKIPTPKPIIEQHNIIVDCSKRHVTMGGKFLELTNAEFNI
                     LEMLIKSPGQAFSKEELTEYALGRKYTAYDRSIDVHISNLRNKLGDNPQGEPLVKTVR
                     GFGYMFNA"
     gene            11972..13339
                     /gene="cpxA"
                     /locus_tag="AC06IO_001976"
     CDS             11972..13339
                     /gene="cpxA"
                     /locus_tag="AC06IO_001976"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CpxA"
                     /protein_id="HIO9875154.1"
                     /translation="MRSLYWKIFVSFWLATILIIFTTAWVTSQIAQKSSQPAREEMFM
                     DSYANAAVATYESGQQSALLKWLDKIGLSRHMTLYLLTSTGEIIGAQKPPDAVKKISE
                     NLINDELPSDGIFKTGKLVVSHEILSTSGKYYRLAAVSEKPISYFVQIPWAGLTIRLT
                     LAIFISGLICYLLSRYLTQPLRSLGMAAKSIAKGNLNTRVGRFRGHSKDEIAELSNEF
                     DRMAEQLEALVHSKERLIQDISHELRSPLARLKIAIELGKKKTKHLADNEFARMEIET
                     SRLNDLIGEILDFARLEKSTTELHLSQTNIADLLAQVISDANYEFSHNSIRIQAGIIT
                     PCELNIDQRLIHRAIENIVRNASHYSPPDEKVLISSSYNKNKDQVYIDINDKGPGVPE
                     DQLEKIFNPFYRVDTSRTKKTGGYGLGLAIAARAVALHQGEIIAKNREQGGLLVRIIL
                     PADVK"
BASE COUNT         4151 a         2669 c         2343 g         4241 t
ORIGIN      
        1 gcctaatatt atagagaatt tgaatttgat atatatccac accatatcgt cccattcctt
       61 cagcccattc ttgctcaatc gctgcctgaa gcaaagtatg caaaaggatt tctgcgtcac
      121 tggcatatcg tccttgtaat acccatgctg agcccagcaa aactcgaccc agcatcactt
      181 caaattcaat tggaacttcc cctaattgtt tttttatttt ttcaagaata ctttgtacac
      241 tgtaatgtgg ctcaccgaca accttataac ctaattggtg tatatcatga tttgtaatag
      301 tagccaccat cccgctatac ccaaactgga attcctttgg tgtcttagcc tctctaataa
      361 tgcttgacac agcatcaccg ctataagctt caatattgcg tggctttcct tgatacacta
      421 atctatccgc tttcgttttg gaaactattc gtgtgagacg ttcattttga acttccgaga
      481 tattttttgt gctaccagat gtttgattac tatcaccata gctatcttca tgcgataaca
      541 ttaaagtttt aaattcaact ttcaggtttg aatcagtatc ctttatcgct ttaacaatgc
      601 gctctataaa atcaggctta ttgcgtaaac caaatcctga accaatattt tccactgatg
      661 cagcaggaca acacatatta ataccaatac gaattttatg atgaaactct ttgggcacca
      721 ttctttttaa catgagaata gcaagctttg ttatttcttc taatttattg ccattttcac
      781 cataaaaaaa gtcgaaatct tcttgcttgg aaaattcatt agggatagcc aactgaaaaa
      841 caaactcttt agctttctct aattcctgac gactaaattg caaacgataa aagctttttg
      901 ttgcgtctga atgatgaagt gtttgttcca aagatgctaa cggagtgctc ataaaccggc
      961 tattgaaaat ttctgcgatt acaatcattt cactataaac tgtacttttg aaaaacccat
     1021 tcaaccaata ttgacgggcg ccaggtccgc ttcctcttac cattggtgcc gttgaaacat
     1081 gagacattaa ctcggattgt tgattggata aaagatttaa atcagttaat gcaggtttgt
     1141 actctgtcga gggttccccg aaaattaaaa catcgtcaag caactcccat gacaggagtt
     1201 ctcctgtttt aaaatttgca tgaaaaacat caagtacttg agtttgctgt tgatgcccct
     1261 taattagaat atgaagctgc tgtttttcgt aaatagctgg caccacagaa aaacaatctc
     1321 ctctaagtgg ggagtttgtt tttatcacag ccaatattgg ctgtctgttt aaaccagtct
     1381 caaaaccatt tggcgtttta aaaaaagatt catctaaagt taagtaaata ttctttttat
     1441 ctgttgacag cttttttgga gcatttctat ttaattcaat actaaagaca ggttcctctc
     1501 taaaataaga ggtaggatca tagttgaata tttccgggta attttttgtt aataacccca
     1561 atattttatt ggttgaacgt ccattaaaaa aatttgcctc agcatgccgc tgccagggag
     1621 taaaagatct aagtggttgc tgtgcggcct tatccggcaa taaatcgtca taaatagcag
     1681 tccatctaag tgttaattga gaaataacgt ggcctgaacc atctatgtgc agaagatatt
     1741 tgcgaaactt cgtatcaaaa caaatggaaa gcccatcttc ttttgagtca tcttttaata
     1801 atataaccca tgatgatttt cgttcatcgc tgcctttaac aggcgcagtt cgataaacaa
     1861 tgctgcctgg atcaagttgc ccctcatcat taattgggaa aacagacgat ccgagtttga
     1921 cataaatggg atggaaagtt tcatatgttt ctctttgaac acgacttaga cccaggttag
     1981 attgtacagc acggcgtatt ttttccatca tattattaaa tttatgcaaa ttctcagact
     2041 ttccctcttc tcctttagat atactacaaa taagtgcttg caaggatttg gcacaaaatt
     2101 caattttttg ctctctttgt atcgcatcag aaggcggaac aactcgacta aggtgaaaac
     2161 tttcattctt aagattacct atcaaatact cagtggcgcg ctcataaaaa atatgcgata
     2221 cacttttgac tctcctgatt tcatctgccg aaagatttct aaaaatagca tttgcaatat
     2281 ttgacttatc taatatcatt ttttctttca tttacaacat tctctttttt aatgcattat
     2341 cgtaatttca tttcaccctt gattgttctt gcatattttg ttcaatttga aactaatact
     2401 ctaaaatttt ataaattatg ctcatagcaa aaaaatcagg ccttgttgta ttcggatatc
     2461 cctcaaaccg catttataga caaatgtgat taagctattt ttatttgggg attattccct
     2521 acttgttcgt aatgctttac gttgagacta gaagcgattg tgctatccaa tctgggaaaa
     2581 gaatttggag ttcgggctta ttctgcattt aaatactctc ttgtttaaaa cataaagaat
     2641 acttaaagca acattcagag aatatccgta aaatcacgga taaaatggtt atttatcttg
     2701 taatgatgcc gttcaattga agaatcaact cagctttatt atcacacccc aattttgttt
     2761 ttaattgatt aacgtgggtt tcaacagttc tagctgataa ttggagtatt ttcccaattt
     2821 ctttataact cattccttgc aacaagagtt ttgcacagtc cttttgccga ggagaaagaa
     2881 gggatctttt tatttcctta ttgattttgt tgctctgaca acaagtagca ttatagggga
     2941 gtattatttt atttttttcg aattgactta ttattgaatt aaattgatcc ttaaagtaaa
     3001 gactatattg ttctagaaca tccagattat tgagataaaa gttattaaca atactggtat
     3061 tatcacgtgt gcttgcaaaa caaaaatatt ctatagaatc atggtttatt ttagcaatag
     3121 atataccatt ggccatatcg aaatttattg ctgcatcttg gagcatcaaa ggacagtcat
     3181 ccgttagcca taaataataa ttcagcgttt tttttaaata ggattctgga atagtcagcg
     3241 tctccagata attcatttta aaataatgct cagtccattt ttggtctgtg gagagtctaa
     3301 tgacagtgct atcaaagtaa attcgataat aattaaagac tgtcatacca tgttttgcga
     3361 gcaaaggttg catgatattt tttatatctt cacaggtagt caaggcaagg tgattttcca
     3421 tatgccttaa aacatttgat gaattaggca ttagctctct cttcaaactc atgggcaaat
     3481 aatcttgcgt agattttcaa atctggattc agcatttttg gaaagccatg taaattgctc
     3541 cagtgcttct tcagatgtta aattttcgtt ccctaaaact tcttcaactt tgaatatgtt
     3601 tatacatgtg aggataggat taatcaaaaa taactgtcca gaaagtcata actcttttca
     3661 agacagttga catagtgaac acaacgaaat ccaacgtctg attttaaatc atttaagaca
     3721 attcattggg taatgaatca agaaattcag tcacttcttt tggggtaagt tcataaaact
     3781 gccctctgga taaaaacctg ggcatagcaa gcatgccgta acggattctt attaatcgac
     3841 taacctcaac gccttgagac tcccacaaac gacgtacttc tctatttctt ccttcattaa
     3901 gggtcacatg ataccaggaa ttagttcctt ctcctccacg aaactggatt ttggtaaatt
     3961 ttgccaaacc gtcatccaac ataacccctt tcaataagtt atttaaagct tcctggctga
     4021 cttgaccatg aactcgtacg gcatactccc tttccaaacc atattttgga tgcatgagtt
     4081 gattggccaa ttcgccatta ttagtaaaaa tgagtaaccc ggaagtattt aaatccagac
     4141 gccccacttg cacccaacgc ccctgtctaa gatgcggtaa gttatcaaat actgtttttt
     4201 caaattttgg atcatgtcga ctggatactt caccgacggg tttatggtaa agaagaaccc
     4261 ggatgttttg tttgcctttt aaagggttgg gaatcaactt tcctttgaca ctaattttat
     4321 cactagggcc agcggaatct cccagtttag ctggtttgcc attaacctgc acccaaccat
     4381 tatcaatcca ccgctccatt tcacgacgag atccgagtcc ggcctgactt aatattttct
     4441 gtaatctttc actgctcatt ttgcgtacac tcttctatag attgggttgc ctcgtgcagg
     4501 gttaatgtat ccattatttc aggtaaggga ggaagttcac ttaggtaatt taagttaaag
     4561 taatttaaaa actcttttgt cgtagtataa acagctggtt ttcccactac atccttatag
     4621 cctgctatac gaatccactc tctctccatt aacgttttca tgatctggct gttaacggca
     4681 actcctctaa tgtcttcaat atcagcgcgc gtaacaggtt gcttgtaagc aattattgcc
     4741 aaagtttcaa gcagtgcgcg cgaatacttt gctggcttct cgatttggag acgagcgacc
     4801 caggcagcat atttcgattt cgtttgtacc ttaaaaccac ttgcaacctg gacaagctca
     4861 aaagcacgag aagaataatc cgcttttaag ctctctatta tagcctgcaa atcttctagt
     4921 gttggtcttt gccactcatc aaaggtctct tggatttttt ctaaagtaac aggttcatta
     4981 ctggataata ccaaagcttc aacaatattt tttaattcat tgtcaatcat ctcatgcagc
     5041 ctgcaaatgt ataggtgaaa atggctgagt ttgcgtaatg atcaataaag actgcttagc
     5101 cagttcaaga atagctaaaa aactcacaac caatcccatc cttccctctt caagagtata
     5161 taattggcta aattccaaaa ttttttgatg ctgcaattga aagagaatac gactcattcg
     5221 ttcacgaaca gacattaact ctcgggaaac ttgatgtttg gaataatgct cttcacgttt
     5281 cagcagaact ttcatagcct cgactaattc agataactca acttcaggat aaactttaat
     5341 tgtttcaacc tcatctggat taacctgaat tgtaaaatta tctctgtctt gtcttggcaa
     5401 tccatccaat aattcagccg cttgttttat ttgctcataa gcttgcaact gtcttaccag
     5461 agtcatgcgt ggatcttctt cctcctcctc tgaatctggt gtaactggca ataataaacg
     5521 tgacttgatt tcagctaaca tggcagccat taataaatac tcggcagcca gctccattct
     5581 tcgattttcc atgagctgga tatattgtaa atactggttg gttatacttg tcatgggaat
     5641 atcaagaata tcaatatttt gcttgcggat taaatacagc aataaatcta atggcccggt
     5701 aaaagaatcc agtaatacct ccaatgcatc aggaggaata tataaatcct ctggaaactg
     5761 agtaatttcc ttaccatcca ccaccgcttt gactacaggc gcaggctgca aatcgatatt
     5821 cattaataat caagccccat gacttctctg accgtattta aagtcttgtt tccttcttct
     5881 ctggccgctt cgcttccttc cgctacaata cgttttaccg aacctaaatc agcttcataa
     5941 tctgaaatcg cttcctgtat tggtttaagt tcttgttgga tagcaccaat cactggtctc
     6001 ttacagtcaa cgcaaccaat tcctgctgtt cggcaacctt tttgcaccca atcttttact
     6061 tcctcattag aatacacttt atggaattgc caaacaggac atttttcagg ctctcctgga
     6121 tctgtacgtt taacacgcgc cggatccgtg ggcatagtca gaattttctt ttcaactaaa
     6181 gctggctcat ctcttagcat gatcgtatta tggtaagatt tggacatttt ctgcccatca
     6241 agacctggca tttttgcagt ttccgtaagc aaaggctgtg gttcacttaa tataattttg
     6301 ccactccctt ccaaatatcc taacaaccgc tctttatctc caatcgacaa attaggctga
     6361 tcttctaaaa gcgcttgtgc tgtcttaatt gattcgtgga tgccatgctc ctgaaattgt
     6421 ctgcgtaatt cggtataaat tttcccattc tttttaccca tctttttgat tgctgcagca
     6481 gccagctcct caaaattagc ctctctgcca taaagatagt taaatcttcg ggctatttct
     6541 cgcgttaact caatatgagc cacttggtct tcacccactg gaacataatc cgcgtggtat
     6601 aaaagtacat cagcactttg tagtaatgga tatcccaaaa aaccataggt tgataaatct
     6661 ttttctttta atttctcctg ctgatcttta taagtaggca ctcgctccaa ccaacctaaa
     6721 ggagtgatca tagacaaaag taaatgtaat tcagcatgct ctggaaccca ggattgaata
     6781 aatatcttac ataaacctgg attaactcca caagctagcc aatcaataat catatcccag
     6841 aggtgttttt caataaaatt ggggtcttca tattgtgttg ttaggccatg ccaatctgct
     6901 gcaaaaaaga aacaatcgta ttgatgttgt aattttatcc aattttttaa aacaccatga
     6961 taatgaccta aatgcaatct tccactgacc cgcattccag aaacgactcg cttattggaa
     7021 gtaaacagtg ctgacatcaa tttcctcttc ttgttataaa ttaatcaatt atttcaaata
     7081 caaaaatttc tttattccct gaattctaac ctgatgcttg cttcaatcat attttatcca
     7141 aattttatca agattagaga catgagatta ataatcaaat gctaactgga caaaataaaa
     7201 ttcaatggcg tcagctctag cctggtagga gcacataaaa ttctctttca ctaaatacta
     7261 acaaaaccgc gactgtattc tattacttaa agggttcagg atctcctttt ccctctctta
     7321 taattatcgg ataatctcca gttaaatcca ctactgtagt gggttcatgg ccacaatttc
     7381 caccatcaat tattaaatca atttggttgc ctaataaatc tttaattgct tcgggctcgc
     7441 ttaatggcgc ttcagccccc ggtaaaatta aagtggtgct cattaaaggg gcttccagac
     7501 actccaataa agacaaagta atcgtgttat caggtactct taaccccaat gttcttcgtt
     7561 ttgggtggag catcaaacga ggcacttcat gtgtagcatt aagaataaag gtataagagc
     7621 ctggagtaaa agccttcaat agtctaaaaa ttggattaga aacctttgca taagtaccta
     7681 actgggataa gtcacgacaa accagggtca tattatgatg cttatccaat tgacgtaagc
     7741 ggcgaatacg ctccagggct gatttattgc ccaaactaca gcccaaagca tagccagaat
     7801 ctgtcgggta aacgataagc cccccttctt caataattga aactgctttt ctcaacaagc
     7861 gtgcttgagg attatctgga tgtaaagcaa aaaactgact cataaagtcc cctgtttcat
     7921 gttccattct tgccaaatag gcgtgcatat tattggtaat tgtctctggc gccccagatt
     7981 tatacaagat tgagcgtcac tatggtaatc tgatcctgat gaggcgagca aatgaaatcg
     8041 ctcacacatc ccggccattt cttgaatttg tacagttgtc atttctccag aaacgacttc
     8101 catgccaaca cctccagcct ctttaaactc cgtgattaac tcacgcaatt ttgaacgcgt
     8161 caatccatat ttaagtggat gagcaattac agcctgtcca ccagaatgag atataccttt
     8221 gaccgcatcg tgcatgctaa tccataaggt aggtatatag gcgcacttgc ctctcccgag
     8281 gtaattttta aaagccgact tcatatcttt tgccttccct tcattaatca gtacttgggc
     8341 aaaatggggt cgacccaccc gtttgtggcc tgccaaaaga caggcttttt cataagcatt
     8401 gtcaatacca accgaatgca aggattctcc aattttctca gcgcgctcta ttcgactttt
     8461 attttgatga gcaattaact cttgtaattc tgctgtgtga ttaatttggt aaccaagtat
     8521 atgaatgtca tacttcttcc aacgagtact gagttctatc cctttaacta ttttaatgtt
     8581 tgtatggatt gcagcgtcat ataaagcgtc atatcctgca acagtatcat gatcagtaag
     8641 agataggcat tgaacttgta aagtagtcgc tctttttatt agttcttctg gagtcagtgc
     8701 gccgtctgaa aaagaactat ggcaatgaag atcgatcata aattgcagct aatttaaaaa
     8761 aatcgaagta tagcataatt aacgtgagtt cgacataaaa ggcttttata acttttatgt
     8821 cgctaaacac acgttatccc gtaaaaaagc aaagcttttt atacgggatc cattcaaaat
     8881 acccaattct atataagaaa aaccaggtaa aaatatgatg ttgtaaagac taaactatcc
     8941 ttttctttga acaacttgca caacatagta cttttgcact attctttaag tatggtcatt
     9001 aatctttaat aaaaactttg agtagtacat taaacagtga aataatcaaa ttaccacctc
     9061 ttaataccag ggaactcgca aacctgtgtg gggttttgca cagtaactta aaaattttag
     9121 aacagcactt caaggtttca ataaagatca aaaatcatag catgataata tgtggtcaat
     9181 caggtgacca ttttgaagag ataaaaaaag tattaaaatt attgtatcct ttaactagcg
     9241 aacctataga taccaaaact ctacgagtct taatcaatga ggattatcaa acagcaatga
     9301 ctcaccatat cagattatct cgaaaatcaa tttctgcacg taacagcaaa caagccgcat
     9361 atctggattc tattaatacg catgatatta catttgccgt agggccggct gggactggta
     9421 aaacctacct ggctgtttcc aaagcaatag aatgttttga aaaaggcgaa gtacaaagac
     9481 ttatttttgt aaggcctgct gtagaagcgg gtgaaaagct cggttttctt cccggtgatc
     9541 tggtagaaaa agtcttacct tacttgaggc ctatctatga cgcactgtat gaaatgatag
     9601 gctttaaaga aactcaaaaa ctgattcaaa cagatatcat tgaaattctc ccattagctt
     9661 tcatgagggg cagaacttta aatgaatctt ttattattct ggatgaggct caaaacacca
     9721 ctatcatgca aatgaaaatg tttttgacac gcatgggctt tggctctaaa acagtcatca
     9781 caggtgatat cactcaagtc gacttgccta aaggcattga ctcgggcctt gcgcatgcca
     9841 ttcaattatt cagacatttt cctgatatca gcattcatac atttaccagt cgtgaagtcg
     9901 tcagacatcc tttggtttca aaaatcgtag attgctatga taatgagatg gaaaggatta
     9961 aaaaatgacc tatcacattg acatacaaaa tgctacaggg aaattattgc ccttgagtga
    10021 agatgaaatc accaagctgg catcactagc tctaagagat cacaagcagg atgctgagct
    10081 aacagttcgt ctggttgatg ttgaagaaat gacttatctt aaccatacct atagaaagaa
    10141 gaacaaacca accaatgttc ttgcatttcc ttgctcacta ccggagaaca ttgaactgga
    10201 atgtcctctt ttgggtgacg tcgttatttg ccctgaagtg ttgcttgcag aaagtgctca
    10261 attcaataaa tcgctacatg cacattggtc tttaatcctg attcatggag tgttgcattt
    10321 attagggtat gatcatataa aagatgaaga agcctctatt atgcaaatgc tcgaagctaa
    10381 attactagct gaattaggtt atgctaatcc atatgaagtc gaggaaaatg aacttgaata
    10441 aattggaaga tggtgggtcg tggttttctc gtttaaaaca gttcttacaa ggagagccac
    10501 aaaatcagga agaattggtc agtttattaa gggacgcgca aatacgctcc ctgattaatt
    10561 cagagacctt ggcaatgatt gagggagcta tccttttctc aaaaatgcgt gttcgagaca
    10621 taatgctgcc taaaaatcaa atggtttgca ttaataagga tgatgatttt aagcatatta
    10681 tatctattgt aactcaaacg ggacattcac gttttcctgt aacgggtgaa aattctgatg
    10741 aagtggttgg cattttgcac gcaaaagatt tgttaaaata tcaaccagaa aatatggaat
    10801 catttgattt attggacatc tgccgccagg taacttttgt acctgaaagc agacgtcttg
    10861 atagtctgct tagtgaattt cgaagtaata gaaatcatat ggctattgtt gttgatgaat
    10921 atggagaggt gtcaggtttc gttaccattg aagatatcat tgaacaaata atcggtgaca
    10981 tagaagatga atttgatatc gatgaagacg cctatattaa agcacatgaa ggacactgct
    11041 atatcatcaa agcccatact ccaatagaag aatttaacga acagctcaac gctgatttta
    11101 gtgacgaaac ctatgataca attggtggca tagtattaaa taaattcggc tatttacctc
    11161 aacgaggtga agtaataatc atagatgatt ttgaatttaa agttcttaat gccgactcga
    11221 gaagaataaa gttattagaa tgtctcgata aaagaagtcc cgagaaacta aattctactg
    11281 ttgaaggata agtaattcat gagcagctct attctcatta ttgatgatga cacagaacta
    11341 acagatttat taacccaata tttggaacca gaaggcttta atgtagtatg tgtgcatgat
    11401 ggagaaaatg gcgttaaaag agctttaaat caggtatttg atgccattat actcgacgtc
    11461 atgctgccta agcttaatgg ttttgaagta ttaaaggcaa taagagagca tttggaaaca
    11521 cctgtattga tgttaactgc tcgtggtgat gatatcgatc gcattgttgg attagaaatt
    11581 ggtgcagatg attacttacc taaaccttgt aatccacgag agttagttgc acgtctgcgt
    11641 gctattttaa gacgcactca aaaaatccca actcccaaac ctattattga acaacataat
    11701 atcattgttg attgctccaa acgccacgtg actatgggag gaaaattcct tgaattaacc
    11761 aatgcggaat ttaatatttt ggagatgtta atcaaatcac cagggcaagc attttccaaa
    11821 gaggaactca ctgaatatgc tctgggaaga aaatacactg cttatgaccg cagtatcgat
    11881 gttcacatca gcaatttgcg aaataaatta ggcgataatc ctcagggaga acctttagtc
    11941 aaaacagtcc gcgggtttgg gtacatgttt aatgcgtagt ctgtactgga aaatatttgt
    12001 ttctttttgg ctggccacca ttttaattat ttttactacg gcatgggtta ccagccaaat
    12061 cgcacaaaaa tcatcacaac cagctcgaga agaaatgttc atggacagct atgccaatgc
    12121 agctgtcgca acctatgagt caggccaaca atcagcactt ttgaaatggc ttgataaaat
    12181 aggcctttct cgtcatatga cactgtattt actaaccagc accggcgaaa tcattggtgc
    12241 acagaaacca cctgatgccg taaaaaaaat ttcagaaaat ttaataaatg atgaactccc
    12301 aagtgatggc atatttaaaa cagggaaatt agtcgttagc catgagatcc tatccacttc
    12361 tggaaaatat taccgactag cagctgtcag tgaaaagcca atctcttatt ttgtgcaaat
    12421 tccatgggct ggactgacaa tacgtttaac tctggccata ttcatcagtg ggcttatctg
    12481 ctatttactc tcacgttatt taactcaacc tttacgctca ttaggcatgg cagcaaaatc
    12541 tatcgctaaa ggaaatttaa atacccgggt tggtcgcttc agaggtcata gtaaagatga
    12601 gattgcggag ttaagtaatg aatttgatcg aatggccgag caattagaag cgcttgttca
    12661 ttccaaagaa cgattaatac aagatatttc ccatgaatta cgctctccat tggctcgact
    12721 taaaatcgcc atagagttag ggaaaaagaa aaccaagcat ttggctgata atgaatttgc
    12781 ccgcatggaa attgaaacat ccagacttaa tgatttgata ggcgaaatcc tcgactttgc
    12841 tcgtttagaa aaatcgacaa cagaacttca tttatcccaa acaaatatcg cggatttgct
    12901 tgcacaggtt ataagtgatg ccaattatga gttcagtcac aattctataa gaatacaagc
    12961 tggaataatt acaccttgtg aattaaatat tgaccaacgc ctaatacaca gagctataga
    13021 aaatattgtt cgtaatgcat cacactattc tcctccagat gaaaaggtcc tgatttcatc
    13081 aagctataat aaaaacaaag atcaagttta tattgatatt aatgataaag gacctggagt
    13141 tcccgaagat caactggaaa aaatatttaa tcctttctac cgcgtggata cctccagaac
    13201 aaaaaaaact ggtggctatg ggcttgggct cgcaattgca gccagggctg tagcattaca
    13261 ccaaggagaa attattgcta aaaatagaga gcaaggtggt ttgcttgttc gtatcatttt
    13321 acctgctgat gttaaatagg atttctatat atcgagttcc tccttattga ctaaaattga
    13381 tttttgactt gcatttttat tatc
//
LOCUS       DBNCWK010000081        13386 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.081, whole genome shotgun sequence.
ACCESSION   DBNCWK010000081 DBNCWK010000000
VERSION     DBNCWK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13386
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.081"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            24..407
                     /locus_tag="AC06IO_001977"
     CDS             24..407
                     /locus_tag="AC06IO_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIO9875155.1"
                     /translation="MYIQKLKEMFAQMVIKKNANLIPEYYHGEFLLYTNDIVTGYDEF
                     LSSHQQYYATEIQYQVEYDEETFLEQGEKIAGRVFITTTRPNEPAKRIEVILIAQYKD
                     EKIYRLWELTYPDWSKLPAFSNQLT"
     gene            633..1187
                     /locus_tag="AC06IO_001978"
     CDS             633..1187
                     /locus_tag="AC06IO_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HIO9875156.1"
                     /translation="MKGIIFNEFLNFVEKSESYTLVDQIIMDSHLKSHGAYTSIGTYS
                     PKELFQLVKALAMKNGKPTSVILQEYGEYLFEVFAKKYPQFFREKKSVFQFLEALETH
                     IHFEVKKLYDHTELPHFECQYHSQNQMEMIYTSSRPLADFAEGLIRGCIKYHKENMTI
                     VRENLPAKTGFKVRFVLTKGDPDE"
     gene            1180..2457
                     /locus_tag="AC06IO_001979"
     CDS             1180..2457
                     /locus_tag="AC06IO_001979"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIO9875157.1"
                     /translation="MSDYEVLLRHLDREIRARKEAEEISEKKISELYRFAQRLEESLK
                     AQKEISKKLKEKTKALKQQIAKTEEERHAKSTLQTILESAIEYSIIAINLKGVILVWN
                     EGARRNYGYEAQELVNKESIEILHTVEDIQSGKVRKFFQTVRREGKAEEIFQRVRKDG
                     SQFTASVTMTIRYDSQGKKVGYVIISKDITEIKEFEEKLIKSNQNLEEFSYIISHDLK
                     APLRAIIQLATWIEEDCGNKLDSLSRKNLQLLRERACRMSGMVDGLLQYARAGRVNME
                     VEWVDTKELIQEIIDILNHDKQFTIIVHESMPQLPTAKLLLSQVLSNLIANSMKHHHC
                     KTGTIEIGVLDKGTFYEFFVADDGPGIEPVYFEKIFRIFQTLKSKDEAETTGIGLTIV
                     KKIVESQNGKIQVDSELGKGARFSFTWPKVPHQ"
     gene            2520..2927
                     /locus_tag="AC06IO_001980"
     CDS             2520..2927
                     /locus_tag="AC06IO_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIO9875158.1"
                     /translation="MDLAADKVDILYLEDDEVDIQSVERVFHKISSLIKIEIAKSGNQ
                     ALDMLYGRNKENKIHPKLILLDINVPKMNGIEFLKELRADSSFTDIEVFVLTAAYTSK
                     DKLAFEPLNIRGHLIKPLDYGEAIKLFWILQSM"
     gene            complement(2939..4354)
                     /gene="ankD"
                     /locus_tag="AC06IO_001981"
     CDS             complement(2939..4354)
                     /gene="ankD"
                     /locus_tag="AC06IO_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkD/LegA15"
                     /protein_id="HIO9875159.1"
                     /translation="MLTPPPDSKITTTDRSLDKLSIPMDMLKQMNESTMEQTKLDELR
                     KKMSLQTEILNKAKADNDMFFRLLIELMSLKLQGELFKEQLSKISKESGYDSVQSALI
                     QATNSEGQSPLQYALQKQDFTTAKYFLDNGAKAGPIEKAVFEIALDSKAAKEFGFPPL
                     PPEKEKLHPVKNFGLVLGIKTTSVDGTPSQFGHIAPTYQLMTDSVSDFAKSHPGNKNF
                     QEIANAFHFSNEASAFKFSTPQRNPEAGQDLSRRIQSGELTTIPVSCKGHAMGLSYVP
                     DGPGSKSGYLVYTNRGLGSKSNEHGTHIFRIEDSSKITSEFINNMTNGHSNGASHDEI
                     MSQIKAAAGNKDPIHHIKQKGQKNDNCTIANSKSNIEGILLCQKAREVGGFDKLTESD
                     KESVKKEYKEFTKHMRVEKVNELAKALKENPQDPDLNNLTKEYLKQHPNADPKLKQTL
                     ETALKQASESSMTLSQPGKTI"
     gene            complement(4558..5187)
                     /locus_tag="AC06IO_001982"
     CDS             complement(4558..5187)
                     /locus_tag="AC06IO_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875160.1"
                     /translation="MTKIVYLQNDPDKMLYYKDSVPKDLKVVEPDAKMMLEILEQKLK
                     LLEVLDLDWFYCAICMELPKEPVFYPTSEGISNLYEKSELVKWFSRDPRNKSDPVGLG
                     LNVTISQYKTPTPKELLDFVIKSTGFAEEVFIEHYKKPQVNKVPPEEELLLEKPDYTR
                     RNEIDSLSHPQQRSCLSLFLYSIFGCKGKRYGTQEKPESLTSNYLPSNH"
     gene            complement(5486..5944)
                     /locus_tag="AC06IO_001983"
     CDS             complement(5486..5944)
                     /locus_tag="AC06IO_001983"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9875161.1"
                     /translation="MLHFKWCEFSELTSKQLYDILALRASVFVVEQHCPYLDPDGRDF
                     FSMHLLGLEENSLVAYLRLFLPTEIENNLTFGRVVTAKSARTKGYGRKLIRELLSYCD
                     IHYPGIRIQCSAQNYLKKFYEEFGFKSYGSVYEEDGIPHIAMQRTLIKPQ"
     gene            6249..6698
                     /locus_tag="AC06IO_001984"
     CDS             6249..6698
                     /locus_tag="AC06IO_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875162.1"
                     /translation="MSFNNFLKTFNEFLLEQGGTTYRAVDHYLKGKDKPLKSVFFAPY
                     SSAPNFLSRAGHVITAPISFSIITLELVSSSLYLSLKSLNSLVFSDKKAAKIHIIDSV
                     VHFAVSLITAIGVIVSPIINLIDLIGGAISTMRVKSEPAEQMRPSVL"
     gene            complement(6746..7240)
                     /locus_tag="AC06IO_001985"
     CDS             complement(6746..7240)
                     /locus_tag="AC06IO_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875163.1"
                     /translation="MAFQRSFFSHIEDDWRYYQNIRAKYSDAIPIPQRKYFEPIHSID
                     SFATLAVRPIEKPLWLGVHTAGFLLKAIIHLVGALVLSPFALIFAVCVPRSELREQTV
                     SSFKSTAAGSIVAAGMACVALLSTLMSLIFNPLYALSRSAATGIDHLNSVTESCCGLT
                     IAKI"
     gene            7464..10231
                     /gene="ankF"
                     /locus_tag="AC06IO_001986"
                     /pseudo
     CDS             7464..10231
                     /gene="ankF"
                     /locus_tag="AC06IO_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkF/LegA14/Ceg31"
     gene            10390..11358
                     /locus_tag="AC06IO_001987"
     CDS             10390..11358
                     /locus_tag="AC06IO_001987"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIO9875164.1"
                     /translation="MKPIDLKEYLQKLKLGFQLLSDLPPEEQIKYLNSIYLAHIKVFP
                     YSNFELRRIANQHPVQRQSLSFFNYTKLLSSEHGGYCYQSAGLLFDALVQLGFKTEYC
                     AARVLVGEPVNSPKVLAIPATHLVLVVTLGDKKFLLDPGLGASAPRLPILITEKDESI
                     LQGKEEFKFYKHDGVHVLERKTSQGWFRLMQTDLIPIDLKTVELNLLKLERYPDTISI
                     RDNKTIVGAITEYGRKSLVWDAQSNQLKFSKHDGDEVIQKTLTSFEDGVQTLVKEFDI
                     DHISAQELKIYCTETNLPQPIKPWTVDLPLDQAELKKMEENLIFRL"
     gene            complement(11437..12165)
                     /locus_tag="AC06IO_001988"
     CDS             complement(11437..12165)
                     /locus_tag="AC06IO_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIO9875165.1"
                     /translation="MTPSISLITSYSLLPAVLMLIGGGIASLYRPGGSITSATQHFAA
                     GVVFAAVAKELLPKIGAYHDPLALIIGFSVGILAMLFLKWLTNRLEDLEQEKTGLSWG
                     LLTAVGIDLLIDGILIGVAFLAGERGGILIAIALAIEIFFLGLSTTATLGARQVNVPI
                     RLLTSVILAILIPVGAALGASLLIHLPLSITNGILSFGVAALLYLVTEELLLEAHEVR
                     ETPYITLCFFIGFLLILLLEGLAA"
     gene            complement(12250..12930)
                     /locus_tag="AC06IO_001989"
     CDS             complement(12250..12930)
                     /locus_tag="AC06IO_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIO9875166.1"
                     /translation="MKPIAHIADKEIISIPVKECGEELIDLKNQNIIVYGPPPETPLT
                     ANDYTKIRKTVYQKLCLAQNDLPNGWRFRLYEGLRSLTVQEILFNQQRDNLRQSKQYD
                     SYEDLFNETCRLVSPVVHLDGTANIPPHSTGAAVDLELIDSYGNLVNLGMAAKDWLIV
                     DPDLCETDSKLISEEAMQNRILMLKIMAKHGFVNYPREWWHFSYGDRLWAYMTEHSTA
                     IYGAIINN"
     gene            complement(13112..13384)
                     /locus_tag="AC06IO_001990"
     CDS             complement(13112..13384)
                     /locus_tag="AC06IO_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875167.1"
                     /translation="MRMITEIETLLKRAGFTYVGNGRGLGEFEKYENHLYHKTVYGSI
                     QQIQLAMDRENDTVRPVFSKNVPVELRDSIYELMKNPSEKNSLTFN"
BASE COUNT         4319 a         2316 c         2602 g         4149 t
ORIGIN      
        1 aaaaattaat ttgagagtag aaaatgtata tacaaaaatt aaaagaaatg tttgctcaaa
       61 tggtcatcaa gaaaaacgcc aatcttattc cagaatatta ccatggggag tttctgctct
      121 ataccaatga tatagtgaca ggctatgatg aatttctaag ctcgcatcaa cagtattatg
      181 cgactgaaat tcaatatcaa gttgaatacg atgaagagac cttccttgag caaggtgaaa
      241 aaatagctgg gcgggtattt attaccacca ctcgacctaa tgagccagcc aagagaatcg
      301 aagtcatttt gattgcccaa tataaagatg aaaagattta tcgattatgg gagttaactt
      361 atccagattg gtccaagtta cctgcttttt caaatcagct aacctagacg attcttttct
      421 cctccaaaca ggggaatatc agaaaagtag ttggtcaaga tttctccgct ttacatggtg
      481 tagcgccacg caggagcgag caatgtttca tggtttaatc aatatgatca aaaaaatgca
      541 aaacattcct gcttgcgtca ggattatatt attgataaca aatttacgac tatacttata
      601 gtattaatca attgatataa aacatcttgt ctatgaaagg aatcatattc aacgaatttc
      661 tcaattttgt agaaaaaagt gaatcctaca ccctggtaga tcaaattatt atggatagtc
      721 atttgaagtc ccatggtgcc tacacctcta tcggtacata ctctcccaaa gaattatttc
      781 aattggttaa agcgcttgct atgaaaaatg gcaaaccaac atcagtgatt ttacaagaat
      841 atggtgagta tttgtttgag gtttttgcaa aaaaatatcc tcaatttttc agggaaaaaa
      901 agtcagtatt tcaatttttg gaagcgcttg aaacacatat tcatttcgaa gtgaaaaaat
      961 tgtatgacca tactgaacta ccccattttg aatgccaata tcacagtcaa aatcaaatgg
     1021 aaatgattta cacttcttcg cgtcctttag ccgattttgc ggaaggttta ataagaggtt
     1081 gtattaaata ccataaagaa aacatgacta ttgttcgtga aaatctgcct gcaaaaacag
     1141 gctttaaggt aagatttgta ttaacaaaag gcgatcctga tgagtgatta tgaagtattg
     1201 ttacgccatc ttgatagaga gatacgcgca aggaaagaag ctgaagaaat aagtgaaaaa
     1261 aaaatatccg aattataccg ttttgcccaa cgtctggaag agagtttaaa ggctcaaaaa
     1321 gaaataagta aaaaattaaa agagaagacc aaggcattaa agcagcaaat cgctaaaaca
     1381 gaagaagaac ggcatgccaa aagtacttta caaactatat tggaaagcgc aattgaatat
     1441 tcaatcatcg caatcaattt gaaaggcgtc atcttggttt ggaatgaagg ggccagacga
     1501 aattacggct acgaggctca agaattagtg aacaaagaga gcattgagat tttgcataca
     1561 gtggaagata tccagtcagg taaagtaaga aagttttttc aaacagtacg tcgggaagga
     1621 aaggcagaag aaatatttca aagagtcaga aaagacgggt cccagttcac agcttccgtt
     1681 actatgacta ttcgctatga ttcgcaagga aaaaaagtag ggtacgttat tatttcaaaa
     1741 gatattaccg aaattaaaga atttgaagaa aagttgatta aaagtaacca gaatcttgaa
     1801 gagttttcat acattatttc ccatgaccta aaagcgccgc taagggcaat tattcaattg
     1861 gcaacgtgga ttgaagagga ttgcggaaat aagttggatt ctctttccag aaaaaatctt
     1921 caattgctca gagaacgtgc ctgtcgtatg agcgggatgg tagatggctt attacaatat
     1981 gcgcgggcag gcagggtcaa tatggaggta gaatgggtag atactaagga gttaattcaa
     2041 gaaataatag atattcttaa ccatgataaa caatttacta ttatagtcca tgaatccatg
     2101 ccccaactgc caacggccaa attacttcta agtcaggttt tgtctaatct gatagcaaat
     2161 agcatgaaac accatcattg taaaacggga acaattgaaa ttggtgtttt ggataaaggc
     2221 acattttatg agttttttgt ggcagatgat ggtccaggta ttgagccggt gtattttgag
     2281 aaaatattca gaatatttca aactttaaaa tccaaagatg aggcagaaac taccgggata
     2341 gggttaacta tagtaaagaa aattgtagaa tctcaaaatg ggaaaattca ggtggattct
     2401 gagttaggta aaggcgcaag attttcattt acatggccta aagttcctca tcaataaagg
     2461 tttaggtcta taattaattt agaaataaaa aataaaatat tgtctcacgg agaataatga
     2521 tggacctcgc agctgataaa gtagatatcc tgtatttgga agatgatgag gttgacattc
     2581 agtctgttga gcgcgtattc cataaaatca gttcattaat caagattgaa atagctaaaa
     2641 gtggaaatca ggcattggac atgctttatg gtcgaaataa agaaaataaa attcatccca
     2701 agttaattct tctcgacatc aatgtaccta aaatgaatgg tattgagttc ttgaaagaat
     2761 taagagccga ctcttccttt acggatatag aggtgtttgt gcttacggcc gcctatacca
     2821 gcaaggataa gctggctttt gagcctttga atattcgagg tcatttgatt aaaccgcttg
     2881 attatggcga ggcaattaaa ctattttgga tattacaatc catgtaggta ctgtagtatt
     2941 aaatggtttt tcctggctgg cttaatgtca tactactttc agaagcttgt tttaatgcag
     3001 tttccaaggt ttgttttagt ttagggtcgg cgtttggatg ttgctttaaa tattctttgg
     3061 tcaaattatt taaatcagga tcctgaggat tttctttaag ggctttggct aattcattga
     3121 ctttttcaac tctcatatgt ttggtaaatt ccttatactc ctttttaaca gattctttgt
     3181 cgctttcagt taatttatcg aaccctccca cttctctggc tttttggcat aatagtattc
     3241 cctctatgtt ggatttggag ttggctattg tgcaattgtc atttttttgt cctttttgtt
     3301 tgatatggtg aatgggatct ttgtttccag cagctgcttt aatttgagac ataatttcat
     3361 catgagaggc accattcgaa tgtccattgg tcatgttatt gataaattcc gaggtgattt
     3421 tactggaatc ctcgattcgg aaaatatggg tgccatgttc attgctcttc gatcccagac
     3481 cgcgattggt atataccagg tagccagatt tggagccagg gccatcgggt acataagaca
     3541 aacccatagc atgtccttta cagcttacag gaattgtggt gagttcgccg ctttggatgc
     3601 gtcttgaaag atcctgacct gcttccgggt ttctttgcgg tgtgctgaat ttgaaggcag
     3661 atgcctcgtt tgaaaaatgg aaggcattgg ctatttcttg aaaattctta ttgccaggat
     3721 gagatttggc gaaatcactg actgaatctg tcatcaattg ataggtaggc gctatatggc
     3781 cgaattgaga gggagtgcca tccacactgg ttgtttttat acccaaaacc agaccaaaat
     3841 ttttgacagg atgcagtttt tctttttcag gtgggagtgg aggaaagcca aactctttag
     3901 ctgccttact gtcaagggct atttcgaaga cggctttttc tatcgggcct gcttttgctc
     3961 cgttatccag aaaatatttt gccgtagtga agtcttgttt ttgcagcgcg tattgtaaag
     4021 ggctttgacc ttctgagttt gtagcttgaa ttaatgcgct ttgtacgctg tcataaccac
     4081 tttctttaga aattttgctt aattgttctt taaataattc accttggagt tttaatgaca
     4141 ttaattcgat aagcaaacga aaaaacatat cattgtctgc tttcgcctta ttgagtattt
     4201 ctgtttgtag agacattttt ttgcgtagtt catcgagttt ggtttgctcc attgttgact
     4261 cattcatttg tttaagcata tccattggaa tacttaattt atccaaactt ctatcggtgg
     4321 ttgtaatttt tgagtcaggt ggaggagtta acatggaaca ccctgattga ttagttgaac
     4381 tatatcatta ttatacatca ggacttacaa aacccccaat ctccttatta aaaaatgttt
     4441 ttaattcaat aattgagttt atctaaactc ccctattttt gtcttgatct ttgggggcgt
     4501 aagtcctgta cataacaaaa aaactgtcta aaaagaccga atttgtcttt attaatttta
     4561 atgattagac ggtaggtaat ttgatgttag agattcgggt ttttcctgtg ttccgtatct
     4621 ctttccttta caaccaaaaa tactgtacaa aaagagactc aagcaggatc tttgctgtgg
     4681 atgggatagg ctatctattt catttcgtct agtataatcg ggtttctcaa gcaaaagctc
     4741 ttcttccgga ggaactttat ttacctgggg ttttttataa tgttcaataa acacctcttc
     4801 agcaaatcct gttgatttaa taacaaaatc aagcaattct tttggagtag gagttttata
     4861 ttgagatata gtaacattta aaccaagtcc aacggggtcg gatttatttc tggggtctct
     4921 tgaaaaccat tttactaatt cacttttttc gtaaagatta ctaataccct ctgatgttgg
     4981 ataaaacacg ggctctttag gaagttccat acaaattgca caataaaacc aatccaaatc
     5041 taatacttca agtagtttta atttttgctc taaaatttcc agcatcattt tggcatctgg
     5101 ctcaaccact tttaaatctt tggggacgct gtctttataa tagagcatct tatccgggtc
     5161 gttttgtaaa taaacaattt tcgtcataac atctccaaag aataatactt taaataacgt
     5221 aagttatgga taaagatgac ataacaacca gtcgataccc agggaacgtt cgggctgagg
     5281 agatgcataa gcatcgtctc gaagccctgg catgaaacat gaaatccgat actaggctgc
     5341 gagataatga ctgattttat ccatagctcg cgttaaataa tattattaat gagtaaacat
     5401 gataaaaagt gtgctttatt gtggcactga tgtcaattta aaatttgatc gggctgatag
     5461 attacttcca ggaaacccgg attggttatt gcggttttat taaggttctt tgcatggcaa
     5521 tatggggaat accatcctcc tcataaaccg agccataact tttaaagccg aattcttcat
     5581 agaatttttt caagtaattt tgggcggagc attgaatgcg aataccaggg taatgtatgt
     5641 cgcaataaga taataattca cgtatcagtt ttcttccata tcctttagtc cgcgctgatt
     5701 tagctgtaac aacacggcca aatgtgagat tgttttcgat ctcagttgga agaaacaggc
     5761 gcaaataagc cacgagagaa ttttcttcca ggcccaataa atgcatggaa aaaaaatctc
     5821 taccgtctgg atcaaggtag ggacagtgct gctctactac aaaaacgctc gctcgtaatg
     5881 ccagaatgtc atagagttgt ttgcttgtta attctgaaaa ctcacaccat ttaaaatgaa
     5941 gcatgaaata ctcccaatcc ggctaaaatc ttcgatgcat tatgctgaag ttatgactct
     6001 gaaatcaagt ggagtaatca tcaggatttt gttaaaaaat gtttttaatt aagtcaatta
     6061 gttaaaatta actaccttat taaaaacatt ttttgcttcc aactggatgt atttggcaag
     6121 ttctggttat accagggcaa atttacaggc aatggattga agtaaaaatc atcatcgaaa
     6181 ttatctttag taattaatgc aaattcatat attatggtga ggattttttt aacagggagt
     6241 atctatcgat gtcgtttaat aatttcttaa aaacattcaa tgaattttta ttagaacaag
     6301 gcggtaccac atatcgggct gttgaccatt atttgaaagg gaaagacaaa cccctcaaat
     6361 cagttttttt tgctccttat tcatctgccc ctaatttttt atccagagca ggccatgtga
     6421 taacggcacc gattagtttt tctattataa ccttagaact tgtttcatcg tctctctatt
     6481 tgagcttaaa gtctctcaac agcctggttt tttctgataa aaaagctgca aagattcata
     6541 ttattgatag cgtggtgcat ttcgctgttt ctcttattac cgcaattggt gtgattgtta
     6601 gccccattat taatttgatt gatttgattg gaggagcaat atctactatg agggtaaaaa
     6661 gtgagccagc agaacagatg agaccatctg ttctctagtt ttcgccagat ttgaaataaa
     6721 taagggttgc gattgaacca cctaatcaga tttttgctat agtcagaccg caacaggatt
     6781 cggttactga attaagatga tcgattcctg tggcggcact tcgacttaat gcatacaacg
     6841 gattaaaaat aagcgacatt aaagttgaga gtaatgcaac acaagccata cctgcagcaa
     6901 caatagatcc ggcagcagta gatttaaatg atgagacagt ttgctctctc aattcggatc
     6961 tcggaacaca taccgcaaag atcaaagcaa atggtgaaag aactaatgcc cctactaagt
     7021 ggattatcgc tttcagcaaa aaacctgccg tgtgaacgcc aagccaaaga ggtttttcaa
     7081 taggccgtac tgctaatgta gcgaatgaat ctatggaatg aatgggttcg aaatattttc
     7141 tttgtggtat aggtatagca tcagagtatt ttgcacgaat gttttgataa taacgccaat
     7201 cgtcttcgat atgagaaaaa aatgatcttt gaaaagccac tggaaactcc ttgttattga
     7261 atcagcggat attaaaaaaa ttggcagtgt ttgtaaataa taggcaagtt cttcattttt
     7321 caaatcttgg gattttcttg ttggcagaga caacaagagg ttttttctgg gcttatttat
     7381 gcccacagtg ttattaattt tgtcttaagc aaactatatt acaatggtgc caaaaaagct
     7441 taaaatgaaa gagtataacg aaattgaaag accaattagc caaaatattc gaaaaatacc
     7501 cctttgttca tttagataaa tcaggtgagc tatcaataat tgtgccttta acaggcgcgg
     7561 ccagtatttc cgacaatttt actgccgatg aaaccaagcc aatttttctt tatttatttg
     7621 gcgataatac gtgcaaatca gatctggaaa gaagcacttt tttttatggt aagcagaaat
     7681 ccggaggtcg tcactcagta aaacccttta ccgaattgtt ctcaagtctg attcatgaat
     7741 taaatggtct caaatcgtct actgaaatcc gtgtggatga gcaactattt cttgaaacaa
     7801 tcatagcaaa tctcaatgag ttgaaagagc aagctctcgc tttcagaaaa ggaaatccag
     7861 gctttgaaga catgaaaaat gattttagtc acagcgatga aatcatggta tcaaagagcg
     7921 agtttgttgt aacggtgtat ccagacagag tgcgacaatt tgatttgcca aacgtaatga
     7981 gtcttataaa ccccggtgta tcccgctaca agcaattact tgcccagtcc gacattaatc
     8041 aatgcctgga agcattgtct cttgtgcaaa agcctgctgc agagggtaac ttggacctga
     8101 ttgctgatgc gctgggaaaa ctggatgcag accccatcga ttacttatgc aaaaaagata
     8161 ataaagatcc tcaagcttta tttaaagcgg tttatgaaat tctgcaaaaa caggatacct
     8221 ctttagaaaa tcaacaatca gctgttttcg aagtcatgga aggcaatttg gtcgctgcct
     8281 ttggagacaa cgaagacttt agtacctggc aaaaggaaga gatagtcgat gccattaaat
     8341 caatatttaa acaagcaaat ttgctgagtg aacaatcagg aaccaaacaa aaaacatttg
     8401 cagatgtcta caatcaggct tttgataaat ataacgaaag cactaatgat gattctgaat
     8461 caggatgtaa acgcaggaat acttatcagc tgtttttatt ttacaaacgt ttctctattt
     8521 gtgttctttg cagttgcgtt taaaaaacaa agaaaaggca aaaaaattcc tgggtttgtt
     8581 tgaagacagg ggtacgttga ataatttggt attacaatta tgtaacgatg aaaaagcgtt
     8641 tatgagaatg atggccgatg aatttgagct gacttctgca gaatacgcgt ctattgctga
     8701 atcaacttat ggcatcgtct tggctcatat tgatgctgaa cattttgatg aactaagaat
     8761 agcatgcagt gctcaattgc tccaggattc caattatatc gtgatgggag ggcgtctttg
     8821 ttggagcacc agttctatta atgaagaggg aaatatacga agtttggagc agcaaaagaa
     8881 aatttcatct gaaaattttg aaatttttta taatcaaaga caatcttata ttgataaatc
     8941 aagagagttt caacagataa aagttctgct agataaagaa gtgaatgacg aagggatagc
     9001 tgaagctgtg gcgtcaaaag ttcgctacct gacgaaatat caaaagtcac aaatcttaag
     9061 tgaagtgatt caaaaaaaga aatacgaaca tgcaaaactt ttaattgaaa actatgccaa
     9121 tcttgttttc ccatccgtgt ttatttcagt gataaagagt agaccggttc atattgattt
     9181 gattcgagcg tttctcaacg cagactctcg tcatgcgttg aatattgacg ggaatgattt
     9241 aattcaggca gcaaaaaaag gagattttga gctggttaaa atcattatag aacatagacc
     9301 ggatttattg gaatccaaag actctttcca acaaactccg ttgctttggg cttgtgtcaa
     9361 tggctatgtg gatatcgtgg aatatttaat ggattcaggt gctgatattc atgtcagaac
     9421 aaaagtgtct gggagtcatc gatataaaat tggagaaacc ccaactgggg gacgaaccgc
     9481 acttgattgg gcgagatgct atgataaaac cgatcctgta aaggcatcaa agataattgc
     9541 cttatttgat acttattatc agcgcatgga agaccatttt aaagccaaca gtgcttataa
     9601 agaccaattc aacaaggctc atttaaccca ggcgataaat aatggggatt taaaattaat
     9661 cgcattgttt ttaaagtatt gtccacacct cgaaaaattt tttactcaag aagatcaact
     9721 acatgcaaaa caacgacata atcaacagat gttgcgttct caaattatgc cgtatcaaaa
     9781 ttcattcaat aaattattag atgaattaca aagaaaagca gaattactga ataaaaaata
     9841 tggtactctt cacgaagcaa ccaatgtcat gcaagcacta attaatgact tatccacagt
     9901 aagaaatgat ttttttatgg atgatattac gcctcaatct tttcaaaaat ttgaaaggaa
     9961 gtgtaaacag gttcttgagg aggccagccc agttttatca actcatcgtg gttggcatgg
    10021 ctatcctgat tttgtcagaa gatttatagg agttattgca accttggcag ttattcctgc
    10081 attggttgta cacttttcat cagcaaaggg atatgtagga gttttctttt ctggcaaaga
    10141 aaatataaaa acagactctt ctgaaaaaat gggtgaattt gcgaataacc tggaaaccct
    10201 aaaaacagaa atatccaata aactggctta gctggatagt ttaaccgctg aaaccaagtg
    10261 tttctttcta ttctgtttgg gctgtggaca aagggttcta tggagccctt gatttttgtt
    10321 ttaataaaac actttatttt caaacttaaa ccagatacga tgtaaaaaag tattatgatt
    10381 tagttttata tgaaacccat cgacttaaag gaatatctcc aaaaattaaa attaggtttt
    10441 caattattat ctgatttacc ccccgaagaa cagattaaat acttaaacag tatttatttg
    10501 gcacatatta aagtttttcc ttattccaat tttgaattac gcagaattgc taatcaacat
    10561 cctgtccaaa gacaatcctt gtcttttttt aattatacaa agctgctttc ttcagagcat
    10621 ggggggtatt gttaccaaag tgcgggtctg ttgtttgatg ccttagtgca attgggattt
    10681 aagacggaat attgtgcggc gcgtgttctt gttggcgaac ctgtgaactc ccctaaggta
    10741 cttgcgatac ctgctactca tcttgtttta gtcgtgactc tgggagacaa aaaatttctt
    10801 ctggatccgg gattgggcgc ttctgctcca aggctcccaa tattgataac agaaaaagac
    10861 gagtccattt tacaaggtaa agaagagttt aaattttata aacatgatgg tgtgcatgtt
    10921 ctggagagga aaacaagtca agggtggttt cgcttgatgc agactgattt gatccccata
    10981 gatctcaaaa cggtagagct gaacttactc aaattggaac gttatccgga tacaatttct
    11041 attcgagaca ataaaacgat tgttggagct attacggaat atggacgtaa atcgttggtt
    11101 tgggacgcac aatcgaatca attgaaattt tcaaaacatg atggtgatga agtcattcaa
    11161 aaaactttaa ccagttttga ggatggagtg caaacacttg ttaaggagtt tgatatcgat
    11221 catatttcag cgcaagagtt gaaaatttac tgtactgaaa cgaatcttcc ccagccaata
    11281 aaaccttgga ctgtggacct cccccttgat caagcagagt taaaaaaaat ggaagaaaat
    11341 ttaattttta gactatagaa aagtgatttg tatttgtttt tccaagctgg ttcagctcat
    11401 catagcttat gtttttatta aaatacctgc ttgcatttat gctgccagcc cttctaacaa
    11461 taaaatcaac aaaaatccaa tgaaaaaaca taaagtaatg taaggggttt cacgaacctc
    11521 atgggcttca agcaataact cctcagtgac cagataaagt aatgccgcaa caccaaatga
    11581 taaaattcca ttggtaatcg atagtggtaa atgaattaat aaactggcgc ccagagcagc
    11641 tcccacaggg atcaatatgg ccaaaatgac actggttaac agacgaattg gcacgttcac
    11701 ttgtcttgcc cctaaagtgg cggtcgtcga taatcccaaa aagaaaattt caatggcaag
    11761 agcaatagcg ataagaattc ctcctcgttc gccagccaaa aaagcaaccc caattaagat
    11821 accgtctatc aataaatcaa ttccaactgc ggttaataat ccccaggata gacctgtttt
    11881 ttcttgttca agatcttcga gcctgttagt caaccatttt agaaataaca tggccagaat
    11941 gcctactgaa aaaccaataa taagagcaag aggatcatgg tatgctccga ttttgggtag
    12001 cagttctttt gctacagcag caaaaacaac acctgctgca aaatgttgag tggcgctggt
    12061 tattgaacca cctggacggt aaaggcttgc gatgccccca ccaattaaca tcaacacggc
    12121 gggtaataaa gaataggagg taataagcga aatagatggg gtcataagag atatttttta
    12181 aggtaatatt gacagaataa tcaaataatt agccaagttc aagaatgaca ggcattgtcg
    12241 cattttggct taattattta tgatagcacc gtagatggca gtagaatgtt ctgtcatata
    12301 ggcccatagg cgatctccat aggaaaaatg ccaccattct ctggggtaat taacaaaacc
    12361 atgcttagcc attattttca gcatcagaat acgattttgc atagcttctt cagaaataag
    12421 ttttgaatcg gtttcgcata aatcagggtc gacgatcaac caatcctttg cggccattcc
    12481 aagattaacc agattaccat aactatcaat gagctcaaga tcgactgcag ccccagtgct
    12541 atgtggtggt atattagctg tcccatccaa atggactact ggagaaacca gacgacaggt
    12601 ttcattaaat aaatcctcat aggaatcgta ctgtttgctt tgacgcaggt tatcacgttg
    12661 ttggttaaac agaatttcct gaacagtcag actacgcaat ccttcatata atctaaagcg
    12721 ccagccattg ggtaaatcat tttgtgccaa gcataatttt tgatatacag ttttacgtat
    12781 tttggtatag tcatttgcag ttaaaggggt ttccggaggg ggcccataaa caataatatt
    12841 ttggttttta agatcaatga gctcttcgcc gcattcttta acagggatag atattatttc
    12901 tttatctgcg atatgtgcta tgggtttcat tatttttcgc aaaacaagca aaattttatt
    12961 gcatggattc gacaagttat ccgcacgtta attatatcat gctaacaaaa aagcaggtta
    13021 ggctttatgc tttaattatt taaaattttt ctgataaaac ctaacaattt gcagttagcc
    13081 atttaaggaa attatggctc aaactctaat gtcaattaaa tgttaacgaa tttttttcag
    13141 atgggttttt catgagttca taaatactgt cacgtagctc tactggaaca ttttttgaaa
    13201 acacaggtct gaccgtgtca ttctctctgt ccatagccag ttggatctgc tgtattgagc
    13261 cataaacagt tttatggtag agatggtttt catatttttc aaattccccc aagcctctgc
    13321 catttcctac ataagtaaac ccggcccttt ttaataatgt ttcaatttca gtgatcattc
    13381 taatac
//
LOCUS       DBNCWK010000082        13373 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.082, whole genome shotgun sequence.
ACCESSION   DBNCWK010000082 DBNCWK010000000
VERSION     DBNCWK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13373
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.082"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(7..1023)
                     /gene="pheS"
                     /locus_tag="AC06IO_001991"
     CDS             complement(7..1023)
                     /gene="pheS"
                     /locus_tag="AC06IO_001991"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIO9875168.1"
                     /translation="MLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLK
                     GLANLSAEEKPKVGQLVNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNH
                     STGSLHPVTQVKHRINDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMHDTFY
                     FGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQ
                     ATLAGLKGLLQDFFAYFFGRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFT
                     GWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTF
                     LRQF"
     gene            complement(1160..1519)
                     /gene="rplT"
                     /locus_tag="AC06IO_001992"
     CDS             complement(1160..1519)
                     /gene="rplT"
                     /locus_tag="AC06IO_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIO9875169.1"
                     /translation="MPRVKRGVTAKARHKKILDQAKGYYGARSRTYRVAKQAVIKAGQ
                     YAYRDRRQKKRQFRALWITRINAQARECGLSYSRLIDGLKKASIELDRKILADMAVHD
                     KVAFAAIAEQAKAALAG"
     gene            complement(1535..1735)
                     /gene="rpmI"
                     /locus_tag="AC06IO_001993"
     CDS             complement(1535..1735)
                     /gene="rpmI"
                     /locus_tag="AC06IO_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIO9875170.1"
                     /translation="MPKLKSHRGAAKRFRKTASGAIKRRGAYRNHILTKKSTKQKRHL
                     RVEAGTLKPCDARLAERMLHGS"
     gene            complement(1756..2292)
                     /gene="infC"
                     /locus_tag="AC06IO_001994"
     CDS             complement(1756..2292)
                     /gene="infC"
                     /locus_tag="AC06IO_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIO9875171.1"
                     /translation="MSAPTKRENDRARINEQITVPEVRLIDVDGNQAGIVSTREALRA
                     AEESGLDLVEISPTAKPPVCRIMDYGKFLFELSKKQAEARKKQKQIQVKELKFRPTTE
                     DGDYQVKLRNLIRFLNHGDKVKITLRFRGREMAHQELGMKILERLQADTADVAIVEQH
                     AKREGRQLLMVLSPKKTK"
     gene            complement(2317..4230)
                     /gene="thrS"
                     /locus_tag="AC06IO_001995"
     CDS             complement(2317..4230)
                     /gene="thrS"
                     /locus_tag="AC06IO_001995"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIO9875172.1"
                     /translation="MPNVKLPDGNVKHFEAPLTIYDVAHHISPGLAKAAIAGRVDGVL
                     VDTSYLIKEDCSLIIVTEKHEDSLEIIRHSTAHLLAQAVKALFPSAQVTIGPVIEDGF
                     YYDFAFERSFTPDDLSLIEAKMHELAKANLSITRRELPRNEAIQYFKGLGEEYKAKII
                     ADIPENESLSLYRQGDFEDLCRGPHVPSTGFLKAFKLTKVAGAYWRGDSNNEMLQRIY
                     GTAWADKKSLEEYLFRLEEAEKRDHRKLGKALDLFHFQDIAPGMVFWHPKGWTIYQEL
                     EHYMRNRLVDFGYQEIRTPQLVDRSLWEKSGHWANFRDEMFVTETENRHYAVKPMSCP
                     CHVQIYNHGLKSYRDLPLRLSEFGNCHRCEPSGALHGLMRVRNMVQDDAHIFCTEDQI
                     QSEVAMMLELVQSVYKDFGFTEIKYRLALRPEKRVGSDDVWDKAETALKLAMQGRNIE
                     WVDAPGEGAFYGPKIECSLSDCLGRIWQCGTIQVDFSMPARLEASYVAEDGSKQTPVM
                     LHRAILGSFERFMGILIEHYAGKLPLWLSPVQAVVLTISEKQNEYAEKVRKTLQKRGV
                     RANFDLRNEKIGFKIREHTLQKIPYLLVVGDKEVENCQVAVRTRDGIDLGVMTIDTIC
                     DTLTQEIIRKGSI"
     gene            complement(4359..4433)
                     /locus_tag="AC06IO_001996"
     tRNA            complement(4359..4433)
                     /locus_tag="AC06IO_001996"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4399..4401),aa:Val,seq:gac)
     gene            4743..5030
                     /locus_tag="AC06IO_001997"
     CDS             4743..5030
                     /locus_tag="AC06IO_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIO9875173.1"
                     /translation="MWFKIIHSHVEIAIYAKPNAKKSKLMAISDDSLHIALHAKPQEG
                     EANNELLFFISQFFKIPKTQIELIKGKSSRHKLIRLPLSESVFCFLNNPKI"
     gene            5366..5851
                     /locus_tag="AC06IO_001998"
     CDS             5366..5851
                     /locus_tag="AC06IO_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9875174.1"
                     /translation="MEKVNLTKQFAYRLRDAMIAAGHNSQRSPSGVCIHKLAEMTGYS
                     LQICRKYLRGEVIPDPVKLIEIASKLNVSPGWLLFGETHNDPGTSKEHITISKSLLHH
                     IFTKAACLYNRELVENDVSDFLMDLINDISLINATEEQSKKIIDLAISSVSHFRRSQG
                     T"
     gene            complement(6550..8112)
                     /gene="wipA"
                     /locus_tag="AC06IO_001999"
     CDS             complement(6550..8112)
                     /gene="wipA"
                     /locus_tag="AC06IO_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipA"
                     /protein_id="HIO9875175.1"
                     /translation="MPKRLINKNINIYNYPNEFEDNLGSISLGDLHGNAIKLIHFLFR
                     HKIIKFKPEISNFHEAYRQFVTIYEQYDDIVQEYLEIRTLLQLIQIKITNAQQRILDI
                     EQKLSLATDHEKEFSQSLLQLKKPIETNLQMAEKSKAGLEEKLSGLKTRLPSCIERFN
                     KFMTQIEINDIKTLIRLLGDEVADRGSCDYFTLRILDFLYQNQIAIKIILSNHGYEFI
                     HAYEKLVVGQPFKPKGYIGDIQIKSFWGLQLLLEQSAITQEELRSLVERAYKPTLKII
                     DYSLSEDGITLYSHAPIRFDSIRMAASQLGVTYNDSTKEALAETIDQLNAQLQIYMKN
                     NMLHLLFENNEINDPTNMTDEERNASPLIYLVWNRWNESKEVENARPGKYNGYFVTYV
                     HGHDPFQSPLTYVYNLDTLCGKYSRVGEEEQINKAFQFLTENRHTNVDKTASESLLRN
                     ISRYKVLDSDENTLKHKIPKTSLALATDILDCKIKESLIKLSLLGKPKAVSGSLGVDQ
                     NISIPNQVSLGK"
     gene            8393..9544
                     /locus_tag="AC06IO_002000"
     CDS             8393..9544
                     /locus_tag="AC06IO_002000"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HIO9875176.1"
                     /translation="MRLLSFKKSKIVSIGTELEFQIIDCSSLSLVARSKELMRALKDM
                     RYRDQIKPEITQSMIEINSSIHQSAKEMYDELLELQKILVETAASIDIAFCGGGTHPF
                     QQWTMQKIFPSKRFKKKFNQYRYLSKRATVFGQHIHIGCPTGDDAIYLTHALARYVPH
                     FIAISASSPFYLGINTNYCSSRSTIFNAFPLSGVIPYLRNWQEFSDYYRKMYRWKIIE
                     NMKDFYWDIRPKPELGTIEIRVCDTPLTLRKSILITAYIQALALYLLEEKPVELSHDL
                     YYVYNYNRFQASRHGLEGELTVTDKDRPIPIMDDILETIKKIEQYINGLGNSEYIEEL
                     YSDVINKQNDSVLINKIYKQDGSFSKLVAAQCELWLSDSKDRKWMTQPS"
     gene            9523..10554
                     /gene="legN"
                     /locus_tag="AC06IO_002001"
     CDS             9523..10554
                     /gene="legN"
                     /locus_tag="AC06IO_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegN"
                     /protein_id="HIO9875177.1"
                     /translation="MDDTTFLSTKEKILEIKPLMESAFGNILGYQELENMLAICTLVP
                     FSNNQILFHQGDRIKGIYIIIKGTVDLIARTLGKGITKIETCLAGDFLGETSFLLNEP
                     SATSAVAKDNVTCLFIPSAYFELMPAFSPEAKYKIMKAIGCQACGRLKQLHDKIIDFI
                     SHSDMQSLSFLGRVIQTFTQPKIHTLDESGVDKDQIYKKTLFHTFTQDETNELLEHAI
                     PLTANKNCVLIHENETLPVCYIVIYGAVQSNVIQNSKIAKLSVIGPDSLFASTACFLS
                     DTKFSITFITCESATLLKISETELQYFQKNKPMIWYKLFNLICGSIISLARSLKKLDV
                     RLNIEKYNQ"
     gene            10561..11478
                     /locus_tag="AC06IO_002002"
     CDS             10561..11478
                     /locus_tag="AC06IO_002002"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIO9875178.1"
                     /translation="MCRILSYLGQPTVVEELLYKPDNSFIKQSYHPKYLPYLLNLAGF
                     GMAAWDRNSINPLQAYVYKTSKLPFYDENLHNLAEKIAPHCLLAHLRGVSYHDKQVVT
                     DQNVHPFLYPVTSIALAHNGMLHNFETMRYDLLKYVKTEYRKYIKGTTDSEWIYAVFL
                     SQLENPKGHFDASEIINAVIATLKILKTVRQQNNIVINSPVNLFLTNGDFIAATRFCL
                     DYGWKPKDAPQSTHFTYHSLWYTYGERYGFYENEYKMKGGTINSSIIIASEPLTENTT
                     TWLEVPEYTLIFANQIDGEIKITSQDINI"
     gene            complement(11606..12661)
                     /locus_tag="AC06IO_002003"
     CDS             complement(11606..12661)
                     /locus_tag="AC06IO_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HIO9875179.1"
                     /translation="MVNSDLILSPYQLNEKITLKNRVIMAPMTRNMANDDFSPTRQMA
                     DYYARRADAGLIITEGTIISPDGQGYSNAPGIFTQTHIDGWKMVTDAVHERGGKIFLQ
                     IWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYAL
                     AARRAMIAGFDGIEIHGANGYLIDQFLHYYTNQRHDQYGQYPENMGRFALEVVKACIN
                     EIGGQSVGLRLSPGAYLNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKE
                     LGFKTMTEFMRAHFKGTLIASGSYTLHQARKDIQNKKYDLIALGRPFIANPDLMNRIK
                     WNADFLPYENRMLDTLF"
     gene            complement(12733..13032)
                     /locus_tag="AC06IO_002004"
     CDS             complement(12733..13032)
                     /locus_tag="AC06IO_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIO9875180.1"
                     /translation="MNVINILKALSNEKRLQIIRWLKEPDKHFSSSHCDVSKDGVCVG
                     LIEKKSGLSQSTVSQYLSQLQQAGLIFTERRGQWTYCKANASLINEFINELKVMI"
BASE COUNT         4143 a         2805 c         2238 g         4187 t
ORIGIN      
        1 taatggttaa aactgccgta aaaaagttaa atcattttca aataacatac gcaaatcatc
       61 tatgccataa taaagcattg ccaatctgtc catacccatg ccaaaagccc atccatgata
      121 ctcattcgga tcaatgttca ctgcgattaa aacgttaggg tggaccatac cacaacccaa
      181 tacctcaagc cagcctgtaa acttgcaaga gcggcaaccc ttaccattac attgagtaca
      241 ttctatatct acttctgcgg aaggttcggt aaaagggaaa taggaaggtc tgaatcttaa
      301 tgccaattcc cgaccaaaaa aataagcgaa gaaatcttgc aacaaaccct ttaacccggc
      361 caaagtggct tgcttatcga tgagtaatcc ctccacttga tgaaacatag gggtatgggt
      421 tacatccgaa tcacaacgat aaactcggcc aggagcaatt aaacgaaaag gtggcttacg
      481 ctgctccatt gtccttattt gtacaggaga ggtgtgtgtc ctcaacaagc gcccgtctcc
      541 aaaataaaac gtatcgtgca tcgcacgtgc aggatgatga ccagggatat ttaatgcttc
      601 aaaattataa aattctgttt caatttcagg accttcaaca atatcaaatc ctaaacgact
      661 aaaataatca ttaattctat gctttacctg agtaacagga tgtagggaac ctgtggaatg
      721 attgcgacct ggaagagtaa catcgatttg ctctgctgcc agcttggcta ataattgctt
      781 ttctttcaat tcgatcattt ttgtttcaat caaagcacta attccctgct ttgcttgatt
      841 gaccaattga cctaccttgg gtttttcctc tgcagacaaa ttagcaagtc cttttagcaa
      901 ttcggtgagc ttgcctttct tgcccaaaaa atcaacacga atttgttcaa gagcaactat
      961 atccgtggct tgtttgattg cttctgaagc ttgttcttgt atggtgttta taagtaccaa
     1021 catagttcta cccacaagta aaaaggaggc cttagcctcc tcaataattt ttattttaca
     1081 aaccggcttt tatttaagat cgctattccg aattgagatt tataaacgaa aggcaataac
     1141 aaataaagcg ggctgaaaat taaccagcta aagctgcttt ggcttgttct gcaatagcag
     1201 caaaggcaac tttatcatga accgccatat cagccaggat tttcctgtcc agctcaatgg
     1261 aagctttttt caaaccatca atcaagcggc tgtaggataa cccacattca cgagcttgag
     1321 cattgatacg agtaatccac aaagcacgga attgtcgctt tttctgcctt ctgtctcggt
     1381 atgcgtattg accggcttta attacagcct gtttagcaac tcgataggta cggctacggg
     1441 caccataata acctttggcc tggtctaaaa ttttcttgtg acgcgctttt gctgtcacac
     1501 cacgttttac tcttggcatt gttcaatctc cccgttaact accgtgcaac atacgttcag
     1561 ccaaacgcgc atcgcatggc ttcaaagtac ctgcttctac acgcaaatga cgcttttgct
     1621 tagtagattt tttagtgagg atatgatttc tgtaagcgcc gcgacgttta atcgctccac
     1681 tcgctgtctt tcggaagcgt ttagctgctc cacgatgtga ctttaacttc ggcataacaa
     1741 tatactccgc attcgttact tggttttttt gggtgataat accatcagta attgtcttcc
     1801 ttcacgtttt gcatgctgct ccactatagc tacatccgcc gtgtcggctt gcaaacgttc
     1861 caaaatcttc ataccgagtt cttgatgagc catttcacga ccgcgaaaac gcaatgtgat
     1921 tttgacctta tcaccatgat tgaggaaacg gatcaggttg cgtagcttga cctgataatc
     1981 tccatcttcc gtcgtaggac ggaattttaa ttctttgacc tgaatttgtt tttgcttctt
     2041 tcttgcttca gcttgttttt tactcagttc aaagagaaac ttaccataat ccataatacg
     2101 acaaacaggg ggtttggctg ttggagatat ttccaccaaa tccaatccgc tttcttctgc
     2161 tgcccttaac gcttctcgcg ttgatacaat tcctgcctgg ttaccatcga catcaattaa
     2221 gcgtacctca ggtacagtga tctgttcatt aattcgtgcg cgatcatttt cacgcttagt
     2281 tggtgcacta atggtttaat cctccagttc gtttatttat attgatcctt tacggataat
     2341 ttcttgcgtt aaggtatcac aaattgtatc tattgtcatc acacccaggt caatgccatc
     2401 tcgagtgcga acagctacct gacaattttc aacctcttta tcgccaacaa ccaataaata
     2461 aggaattttt tgtaaagtat gctcgcgaat tttaaatcca atcttttcat ttctcaagtc
     2521 aaaattggcg cgaacccctc ttttttgcaa agttttcctc actttttcgg catactcatt
     2581 ctgcttctca ctaatagtaa gcacaaccgc ttgcactggg gataaccata atggcaattt
     2641 acctgcataa tgttcgatta atattcccat aaatcgttcg aaagaaccca aaatggctct
     2701 atgcaacatc accggtgttt gcttgcttcc atcttcagca acatagcttg cctcaaggcg
     2761 tgcaggcata gagaaatcca cttgaatcgt tccgcactgc caaattctac ctaaacaatc
     2821 agagagagag cattcaattt taggtccata aaatgctcct tctccaggcg catccaccca
     2881 ctcaatattt cttccctgca tagccagttt aagggcggtt tccgccttat cccaaacgtc
     2941 atctgaaccg actcgttttt cagggcgcaa agccaatcgg tattttattt cagtaaaccc
     3001 aaagtcctta tatacggatt gcactaactc cagcatcata gccacttcag attgaatctg
     3061 atcctcagtg caaaaaatat gagcatcatc ctgcaccata ttgcgtaccc gcatcagccc
     3121 atgcagtgcg cctgatggct cacagcgatg acaattccca aactcagata gtctgagagg
     3181 aagatcccgg taacttttca aaccatgatt ataaatttga acatggcagg gacagctcat
     3241 aggttttact gcataatgtc gattctctgt ttcggtaaca aacatttcat ctctaaagtt
     3301 cgcccaatga cctgatttct cccataacga tctgtctact aattgcggtg ttctaatttc
     3361 ctgatagcca aaatcgacca ggcgatttcg catgtaatgc tctagctctt gataaatagt
     3421 ccatcctttg gggtgccaaa aaaccattcc cggagcaata tcctgaaaat gaaacaaatc
     3481 cagtgccttg cctaatttac gatgatcacg tttttctgct tcttccaaac gaaacaaata
     3541 ttcttccagg gattttttat cagcccaggc agtgccgtaa attcgctgca acatttcgtt
     3601 atttgagtca ccacgccaat aagcgccagc tacttttgtc aatttaaatg cttttaaaaa
     3661 cccagtggaa gggacatgtg gacctcgaca caaatcttca aaatcacctt gcctatatag
     3721 agagagagat tcattttcgg gtatatcagc aataattttt gccttatatt cttcgcctaa
     3781 acccttaaaa tattgtattg cttcatttct aggtaattct cgacgagtga ttgaaagatt
     3841 agcctttgcc agctcatgca tcttcgcttc gattaaactc aaatcatcag gagtaaacga
     3901 gcgctcaaaa gcaaaatcat aataaaaccc gtcttctatt acaggaccta tagtcacttg
     3961 agctgatgga aataaggctt ttacagcctg ggctaataaa tgagcggtag aatgcctgat
     4021 tatttcaaga ctgtcttcat gtttctcggt tactataatc aaagagcagt cttcttttat
     4081 aagataggat gtatctacca agacgccatc gacacggcct gctatcgctg ctttagctaa
     4141 tccagggcta atgtgatgtg ccacatcata aatagtgagt ggcgcttcaa aatgttttac
     4201 atttccatct ggtaatttaa catttggcat aatcttatcc gtctttccat ctttcaggtt
     4261 cttgttaaaa aaaaaccctg catggcaggg ttcaaacctt taataattca gtgcagtagt
     4321 ttaccaattt agggattcta ctagaaatcc aggaattatg gtaggcacga gtgggatcga
     4381 accaccgacc accaccatgt caaggtggtg ctctaccact gagctacgtg cctataattc
     4441 aacctgaatg aaaattggca tgaattatac acataagatt catggaaatg caatcatcca
     4501 aatcatatca tttttatcta atgaaaaagc aagataacat gacattaaaa ttttggatga
     4561 gcatggccta tcaatcttgc cttttaactt ttttgcaaat gcaacccttc tcaactaaac
     4621 tgcaaaacac tcttgctttc cccaaaatgc attagtgaaa gaataaagag cacgaaaaga
     4681 taattccaac tctcttggga acaaggtaaa atgataaagc ttccgttagc atttaaaata
     4741 ttatgtggtt taaaatcata cattcccatg tagaaatagc tatttatgcc aaaccgaacg
     4801 ccaagaagtc aaaactcatg gccatttctg atgacagtct gcatattgcg ctccatgcca
     4861 aaccgcaaga aggggaagcg aataatgagc ttttattctt tatatcccaa tttttcaaaa
     4921 ttccaaaaac acaaatcgag ctaattaaag gaaaaagcag ccgtcataag ctaataagat
     4981 tacccttatc agaatctgtg ttctgctttc taaataatcc aaaaatctag aacatatcac
     5041 cctttaaaac ttagaaatgg cccgaatgaa ggggttcatt caagccatgc aatgcgaggt
     5101 taaccgctat ggttaaacca ctttttctat tgattggact acccggtgat acttgttcct
     5161 tcaagtaaca tcagcgtccg atatttggac ttaaagaaac gtgttataac gttccaacac
     5221 ccattataat catttgacac caaatatcaa taaatgattt ctaatggaaa caatttgttt
     5281 cttttaaatg gatatatttg caggtttaac taccctttgc tacacttttg ccactttatt
     5341 aatatcttaa aattattact ccaatatgga aaaagttaat ttgacaaaac agtttgctta
     5401 ccgcttgcgt gatgccatga ttgcagcggg gcataactct caacgctctc catcaggagt
     5461 atgtatccat aaattagctg aaatgacagg ttactctttg caaatatgca gaaaatacct
     5521 tcgtggagag gtcattcccg atccagtcaa gctgatagaa attgcatcga aactaaatgt
     5581 ttcgccagga tggcttttgt ttggtgaaac ccataatgat cctggaacat ctaaggaaca
     5641 catcacgata agcaaaagtt tactccatca tatttttaca aaagcagctt gtctatataa
     5701 tcgagagctt gtggaaaatg atgtgtctga ctttttgatg gatttgatca atgacattag
     5761 tctcatcaat gccaccgaag aacagtccaa aaaaataatt gatttggcta tctcttcagt
     5821 gagccatttt agacgctcgc aaggaacgta gagtaagaaa ctttcatgga aaatagccca
     5881 aaagagatag tgccccactc tcgactttta gagaaccttt tcgcctttaa aagtaaagta
     5941 tccaatgtct ttagagacgt attgggcgta catgacgttc atcatatttc cttaacgtgt
     6001 aattaaagaa tacaatcaat tgctgcccct atcctctcct cctgccatgg aatgtaactt
     6061 atttaacagt tttctttggc aacatgagct aacctataat cccaagtaat ttaaattatg
     6121 tactcaaggc gattggcaca cactttaaat ataaaacact atgatgagct atattatctt
     6181 gatcaaatta aacatggttt cccctccctt gctaccaaga taaatgaaaa ttatgcgatt
     6241 tattcaatag caagccacaa atcttgtgcc tttactcatg agttattttc tactcatcag
     6301 gaagattttt aaaaaacagg gccatattgc tccaatttgc tgcatccatt ttttagttat
     6361 tatgaagatt ttccttctca atatttaatt ttaaactcac actgaacaat aagagtccat
     6421 accgacttta taatgaatta cttgattttt aaagagctcg ataatctacg tacgtacctc
     6481 aattttttcg aagcattttg tgtgcatttt atggctatct ctaaaactca gtgcttcata
     6541 gagagtcggt catttgccca gagacacctg attaggaata gaaatattct ggtctacacc
     6601 aagcgaacct gatactgctt ttggtttccc gagcaagctc agttttatta aactctcctt
     6661 aattttacaa tcgagaatat ctgttgccaa tgcaagtgac gtcttaggga ttttgtgctt
     6721 taaagtgttt tcatccgaat ccagcacttt ataccttgat atattgcgaa gaaggctttc
     6781 acttgctgtt ttatccacat tggtgtgtcg attttcagtc aaaaactgga acgccttgtt
     6841 aatttgctct tcctcaccca ctcttgagta ttttccacac agagtatcca ggttatacac
     6901 ataagttaat gggctttgaa aaggatcatg cccatgaaca tacgtgacaa aatagccatt
     6961 gtattttcct ggtcgagcat tctctacttc tttgctttca ttccagcgat tccatactaa
     7021 ataaattaac ggcgatgcgt ttctctcttc atcagtcata ttggttggat cgttaatttc
     7081 attattctca aataacaaat gcagcatgtt gttcttcata taaatttgca attgggcatt
     7141 aagttggtct atggtttcag ccaatgcttc tttggtggaa tcattataag tgacacccaa
     7201 ctgagaagct gccattcgaa tgctatcaaa gcgtatggga gcatggctat acaaggtaat
     7261 accgtcctcg cttagagagt aatcgataat ttttaaagtt ggtttataag ctctctcgac
     7321 taaacttctc aattcctcct gagtaatggc actttgctcc agtaataatt ggagacccca
     7381 gaatgattta atttgtatat ccccaatata acctttgggt ttaaatggtt gaccgactac
     7441 aagtttctca taagcatgaa taaactcgta cccatgattg gataaaatga tcttgattgc
     7501 aatctgattt tgataaagga agtccagtat tcttaaagtg aaataatcac aactcccccg
     7561 atcggccacc tcatcaccca gcaatcgaat aagcgtttta atatcattaa tttctatctg
     7621 cgtcatgaac ttattaaatc gctcaataca tgatggcagc cttgttttca gcccacttaa
     7681 tttttcttcc aggccagctt tactcttttc agccatttgt aaattggtct ctattggctt
     7741 cttaagttgc aataatgatt gagaaaactc tttctcatgg tctgtcgcaa gtgataattt
     7801 ttgctcaatg tctaaaattc tttgctgtgc gttagttatt tttatttgta tcaattgaag
     7861 aagcgtacga atttccaaat actcttggac tatatcgtca tattgttcat aaattgttac
     7921 aaattgccgg taagcttcgt ggaaattgct aatctctggt ttaaatttaa ttattttatg
     7981 acgaaaaaga aaatggataa gcttgattgc attgccatgc aagtcgccca gtgaaatgga
     8041 acctaaatta tcttcgaatt cattaggata attatatata ttgatattct tgttaatgag
     8101 acgttttggc attccctagt ccatgtaaaa aaatcccctt caaaataagg gcatactcaa
     8161 agtcgacaat aacaaatatt cacttttcat gtaataacat ggaagtatta aattcatgca
     8221 atgaactccc actttaatta ctctggaata gcagcaaaca agctaaatta ttgaacaatt
     8281 tttatgggtt tggggaaaca tggaccttcg tatctattcc cattaaacaa tcatctaacc
     8341 aaaaaaccta tctgctatac ttaaacaagg aaaataatag tcagagaaag gaatgagatt
     8401 attatctttt aaaaaatcca aaattgtttc aattggaact gaattggagt ttcagattat
     8461 tgattgcagt tcattgtcgc tggttgctcg ttcaaaagaa ttgatgagag ccttaaagga
     8521 catgcgctat cgcgaccaaa ttaagccgga aattacgcaa agcatgattg aaatcaattc
     8581 atcaattcat caatccgcta aagaaatgta tgacgaactg ttagagctac aaaaaatttt
     8641 agtagaaact gctgcaagca ttgatattgc tttttgcggc ggagggacac acccatttca
     8701 acaatggacc atgcaaaaaa tttttccatc gaaacgattc aaaaaaaaat ttaaccaata
     8761 ccgttatctc agcaagcgcg ctactgtttt tggacaacat attcatatcg gctgcccaac
     8821 aggagacgat gctatttatt taactcatgc cttggcaaga tatgtccctc actttattgc
     8881 aatcagcgct tcatcgcctt tctatctggg tattaataca aattattgct catcacgctc
     8941 gactattttt aatgcttttc cattaagtgg tgtcattccc tatctgagaa attggcaaga
     9001 attctctgat tattatcgca aaatgtatcg ttggaaaatc attgaaaata tgaaagactt
     9061 ttattgggac attagaccaa aaccagaact tggtacaata gaaataagag tctgtgacac
     9121 ccctttaact cttagaaaat cgattcttat tactgcttat attcaagcat tggcactcta
     9181 tctcctcgaa gaaaaacctg tcgagctgtc tcatgattta tactatgtct ataattacaa
     9241 caggttccaa gccagcagac atggtctgga aggagaatta actgtcactg ataaagacag
     9301 gcccatacct atcatggatg acattcttga aacaataaaa aaaattgaac aatacattaa
     9361 tggacttgga aacagcgaat atattgagga actgtatagt gacgtcatca ataaacaaaa
     9421 tgatagcgtt ctcataaata aaatttataa acaagatggc tcatttagca agctggtagc
     9481 agcacagtgc gaactatggt taagtgattc aaaggatagg aaatggatga cacaaccttc
     9541 ttaagtacga aagaaaaaat cctggaaatc aaaccattaa tggaaagtgc tttcggcaat
     9601 attctaggat atcaagaact ggagaatatg ttggcaatct gtacacttgt accattttcc
     9661 aacaatcaaa tcttgtttca tcaaggggat agaataaaag gtatttacat cattataaaa
     9721 ggaactgttg atcttattgc cagaacctta ggcaaaggaa taaccaagat agaaacttgc
     9781 cttgccggtg attttttggg agaaacttcc tttctcctca atgagcccag tgccacatca
     9841 gcggttgcta aggacaatgt gacatgctta tttatccctt ctgcctattt tgaactgatg
     9901 ccggcatttt cacctgaggc caaatacaaa atcatgaaag caattggttg tcaggcctgt
     9961 ggtcgtttaa aacaattaca cgacaaaatc attgatttta tttctcattc tgatatgcaa
    10021 agcttatcat ttttggggcg agtcattcag actttcacac aacctaaaat acacaccctg
    10081 gatgagagtg gagttgataa agaccaaatt tataaaaaga ctttatttca cacttttact
    10141 caagatgaaa ccaatgagtt gcttgaacac gcgataccac taactgccaa taaaaattgc
    10201 gtattgattc acgagaatga aaccctaccg gtatgttata tagtgatcta tggggcagtt
    10261 cagtccaatg tgatacaaaa cagtaaaatt gccaaattgt ctgttattgg tcctgactcg
    10321 ttgtttgcca gtacggcctg ttttctgagt gacactaaat tttcaattac atttattacc
    10381 tgcgaatcag caactttatt aaaaatatct gaaaccgaac ttcaatattt ccaaaaaaat
    10441 aaacccatga tctggtacaa attatttaat ttgatatgtg gttctatcat ttcccttgct
    10501 cgttccttaa aaaaattgga cgtgcgacta aacatagaaa aatataatca gtgaggtgct
    10561 atgtgcagaa tattgtctta tcttgggcaa cccacagtgg ttgaagagtt actttataaa
    10621 ccggataact cctttattaa acagagttat catccaaaat atttgcctta tttattaaat
    10681 ctggcgggtt ttggtatggc tgcttgggat agaaattcaa ttaacccctt acaagcttat
    10741 gtctataaaa catccaaact tcctttttac gatgaaaatt tacataattt agcagaaaaa
    10801 atagcccctc actgccttct cgcccatcta cgtggagttt catatcatga taaacaagta
    10861 gttactgatc aaaacgtgca tccatttctc tatccagtaa ccagtattgc tttagcacac
    10921 aatggtatgc tccataactt tgaaaccatg cgctatgatc tgctgaaata tgttaaaacg
    10981 gaatacagaa aatatattaa aggaactaca gatagtgagt ggatctatgc tgtttttctt
    11041 tctcaattag aaaatcccaa gggtcatttc gatgcctctg aaatcataaa tgctgtgatt
    11101 gccactttaa aaattttaaa gacggtacgc caacaaaaca acatagtcat taactcacca
    11161 gttaatcttt ttttgaccaa tggtgatttt attgccgcga ctcgattttg tttagattac
    11221 ggctggaaac ccaaagacgc tcctcaatcg acacatttca cctatcattc actatggtac
    11281 acttatggag aacgatatgg attttatgaa aatgaatata aaatgaaagg gggaaccatt
    11341 aattcaagca ttattattgc gtctgagcca ttaactgaaa atacaacaac ctggcttgag
    11401 gtccccgaat atactttgat ttttgcgaat caaattgacg gcgaaatcaa aattacttcc
    11461 caggatatta acatttgaaa tgctccatat ttagcatccc aagacgcttt ctcatcatct
    11521 ccttgtgcct tcttcatcac ttcattccaa aattttccgg taatacagaa agcaagaaag
    11581 gtaattgaat accccgctca agtacctaaa acaaggtgtc taacattctg ttttcataag
    11641 gtagaaaatc ggcattccac ttgatacgat tcattaaatc gggatttgca ataaatggcc
    11701 ttcccaaagc aattaaatcg tattttttat tttgaatatc ttttcttgcc tggtgaagcg
    11761 tatagctacc gctggcaatt aaggttcctt taaaatgagc tctcataaat tctgtcatcg
    11821 ttttgaaccc taattctttg aattgaatac cgtcatcaaa attacctgta tgcacataag
    11881 ctattccata gtgattcaat tcttccaata catatcgaaa taccatggca tcccgctcat
    11941 ctccgacaat ttcattaaga taagctccgg gagaaaggcg caatcctaca gattgcccac
    12001 ctatttcatt gatacaggct tttaccactt ccagcgcaaa tcgccccata ttctctggat
    12061 attgtccata ctgatcatga cgttgattgg tatagtaatg taaaaactga tcaatcaaat
    12121 aaccattggc accatgaatc tcaatgccat caaacccggc tatcatcgct cttcttgcag
    12181 ccaacgcata gttttggatt aactcattaa tttcctctaa tgtagccgcc cttgattttc
    12241 cataataaag atcatcggca cgccggacct tacctgtcat catcgtttct gaagcagaaa
    12301 tcggtaagtt accatgtaaa aaaatcggat gagataccct gccaacatgc caaatttgca
    12361 aaaatatttt ccctccccgt tcatgcacag catcagttac cattttccaa ccgtcaatat
    12421 gggtttgcgt aaatattcct ggagcattac tataaccctg accatcaggg ctaataatag
    12481 tgccttctgt aataatcaac ccggcatcag cccttcgagc ataataatct gccatctgcc
    12541 tggttggact gaaatcatcg ttagccatat tacgagtcat gggagccata atgactctgt
    12601 tttttagtgt aattttctca ttaagctgat aaggacttaa aattaaatcg gagttaacca
    12661 tgataaatct cctgcatgtc ataacaatat attggaatat cgcaaatatt cgatttatta
    12721 aagcaaattt ttttaaatca taactttcaa ttcatttata aattcattaa ttaggcttgc
    12781 attcgcctta caataggtcc actgtcctct acgctcagtg aatatcaaac cagcttgctg
    12841 taattgtgat aaatattgag ataccgtgga ttgagataag cctgattttt tttctattaa
    12901 gccaacacaa acaccatctt tgctcacatc acagtgcgac gaactgaaat gtttatctgg
    12961 ctcttttagc cacctgatta tctgtaatcg cttttcatta gaaagcgctt ttaatatatt
    13021 tattacgttc atattttaat tatatcgttt tattgcgata tgtcaattta gtgattaccc
    13081 atggtcatgt gtgtttaatt tcaaacaacc tgcccgataa caacactaaa aataagacga
    13141 atgagagaga taaatgatca atatgggtag aaatagttcc tgcgacaaat ggtattgcag
    13201 aagagcttgt tgatttaact attttacact ctcaaaattt attttaagag cctttaaaat
    13261 ctattttcat tcaggcaatt tatcaaaact tttcatgacg gattcattaa actctttcat
    13321 tacatcactt tcataaaaat cttttttaaa ttcttcatcc tgaatatcaa cca
//
LOCUS       DBNCWK010000083        13352 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.083, whole genome shotgun sequence.
ACCESSION   DBNCWK010000083 DBNCWK010000000
VERSION     DBNCWK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13352
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.083"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            110..286
                     /locus_tag="AC06IO_002005"
     CDS             110..286
                     /locus_tag="AC06IO_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HIO9875181.1"
                     /translation="MKSIEDFTGFSESGIDDAIQNALKKAGNPTHFEVVETLGSQDSQ
                     SDRRYHVTLKTLSD"
     gene            397..1896
                     /locus_tag="AC06IO_002006"
     CDS             397..1896
                     /locus_tag="AC06IO_002006"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIO9875182.1"
                     /translation="MGYNVPHYIGGQLINETDTEAQAIYNPALGEIIGQVHFASKATC
                     DRAVAIAKAAGNEWSQTPAIKRARVLFRFRELLEKYQMDLARIVTREHGKTLDDAKGS
                     VARGIEVVELHCGLVNELKGDFSAEVASHIDCHTFRQPLGVCAGISPFNFPVMVPIWM
                     MIPAIACGNTFILKPSEQDPSAPVRLLELLSDAGLPPGVANCIQGNKTTVEHLLAHPD
                     IAAFTAVASTPVAEYIYTTAAAYGKRAHTFGGAKNHCVVMPDADLDQAANAIVGAAYG
                     SAGERCMALSVVVTVGQHTADALINKISPLIRAIRVDAGDVEGTDMGPLVSQAHRQRV
                     LAAIEKGVNEGARLIIDGRTFQHPQYPQGFYLGPCLFDEVTEEMSVYQNEIFGPVLVI
                     VRVNHFEEALALVNKHQYGNGTAIFTRDGFTAREYSQRVQAGLVGINIPIPVPIANHP
                     FGGWKRSVFGDTNMHGEESIHFYTRRKTVTSKWPTTELKDSSFAMPTHD"
     gene            1920..2816
                     /gene="mmsB"
                     /locus_tag="AC06IO_002007"
     CDS             1920..2816
                     /gene="mmsB"
                     /locus_tag="AC06IO_002007"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HIO9875183.1"
                     /translation="MANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQG
                     GAIAHNLQELAKGKDVIVTMLQTGQQVLHVCLGVDGIFSQLNAGSLYIDCSTIDVKSS
                     RETHQIAKENHLLVVDAPVSGGVAGAAAATLTFMVGGEAEDYNAAHPILSAMGKKIIH
                     TGGAGSGQAAKICNNMILGTTMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSK
                     YVPVPGIVENVPANNDYKPGFAANMMLKDLLLSQNTAQSVHIETPLGAKTTEIYQQFV
                     DQGLGDIDFSAIIKHIGKTQEV"
     gene            2818..4701
                     /gene="acs"
                     /locus_tag="AC06IO_002008"
     CDS             2818..4701
                     /gene="acs"
                     /locus_tag="AC06IO_002008"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIO9875184.1"
                     /translation="MLPVHLKHPHLTPTEESLSEFWSEIAAQTIDWIQPWSITLQGEL
                     HKGDVKWFQGGLLNVSANCLDRHLPHKANQTAIIWEGDDENQNKTLTFAQLYSEVCKM
                     SNVLKSLNVRKGDTVGIYLPMIPEAAIAMLACARIGAIHTVVFAGFSAHALQQRLIAS
                     SCKCLITADAFQRGGKTIPLKKQADEASVDLNITKLVVKNSNAPTALNKNKEHWWHEL
                     KQTVSDQCTPEPMNAEDPLFILYTSGSTGQPKGVVHTTGGYLVQTAYTHQLIFACQDN
                     EVFWCTADVGWITGHSYVVYGPLCNGITTLMFEGIPTWPDAARNWRIIDKHQVNVFYT
                     APTAIRSLMRAGDQWLNSSSRSSLRLLGSVGEPINPEAWNWYHQKVGQGKCPIVDTWW
                     QTETGAIMISPRASDGVIKPGSACKPIPGIVPLLLNEQGHEINGAGEGLLAIKYPWPS
                     MARTIAGDHQRYCNTYLSNGYYITGDGAKRDDDGDYWITGRIDDVLNVSGHRLGTAEI
                     ESALVSHPKVAEAGVVGIPHDLKGQGIFAYVILKQGNKPDAKLQTELMERVKDEISAI
                     AKPDVIQFVNDLPKTRSGKIMRRILRKIACKEVSHIDELGDLTTLANPQIVEELMKNI
                     LKR"
     gene            complement(4797..8105)
                     /gene="cegC2"
                     /locus_tag="AC06IO_002009"
     CDS             complement(4797..8105)
                     /gene="cegC2"
                     /locus_tag="AC06IO_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC2"
                     /protein_id="HIO9875185.1"
                     /translation="MTPLFPTQGPITIRQGIGGSCYLLSSLDCILNLGADGEQLIKSL
                     FTQTEDGKVIVRIKRHEALKDNLQKNKMTGKYTHYVDELNNEDVFEISPERLKEIDNQ
                     YGGVKSNSLAIKILERLVSYYYAGDWSNTDPLASVVAHDIPDRIAGFTSTAFLGKFFG
                     IQAEDIPYSKLDDIIKLKLMNPDEPVYISMSYGKVDSFGKFHGRHALRIDKIIPKGSG
                     NYDFVLINPHDNSKTETYKLDDLNKRNCRFCLFNTSIHRASLTKKLLTLSNEEGKYVF
                     ANSGLQKRLISLEEMNLLTDNKIISSCISLHKQIPYLEKLFLKLSVEEKKTLTTCIVN
                     ADGSKKEFLKLFLTRIPAMDLLELVLREETSQELLGEVLTELALSSPVEENKLSPKAG
                     INFNGEAFLHLIVKSAIQQKINQLAYMPEKAKQEIESGLINFYFGGSSSSLTRASGLR
                     ALFIANVFSKKSIEALFPPKALFAKAIANYLTLKTLPDLLIKYLKSKDTSSIDEEFFD
                     VVLASATFKDPDEFFESLFRLSRINPEVAKALFVFASQKINVLFSISLEEYAKKIALK
                     DSGEFKSWFESLSKPQPVIKIPEIDNVLRQQRVDDAKRVISDIVQRINSFPFSFEGFK
                     TVEHVNLNAEELRGQLKKIVHSGELQNALQILDFPDGHPEVQRALERKLRMIDAAANQ
                     RSDFLRKYETDIDEHVRQIKNFPIDFNDADTIVAIESRRILLNKKLHTQVKAEDLLGE
                     QFIANPKIKMVYYAQVEKINLRAELLQKRLLDEAQKVIDSVEKRIDNFVIRFNDISST
                     SAVEWQRNNLLQQLDNLVKPNQALLSSEKVLDCNDLQPSIVRALQAKKQEINETADQL
                     IIKINAEEVVKSYEKQIREFPISFSRCQTVEEVIARKQDLIQSVRYLVDNKPDLLKAQ
                     EHLQLSDEYHSDIKIALTDKICEINRQADVMSKRITDQIAAIKETLNILAEIKFSDHL
                     KTIESMVKTLETKAVSDENYKRAAPIARTFYNNLLRAEEHFKNSQLPKNVKCNDFHQA
                     CVRAINAVIPVLEVHRGWKQVFADLASALVTLCTLGGANLYAGRWRLFPVPTESEKIV
                     KDFSLSMQPLAVRA"
     gene            complement(8219..8821)
                     /gene="yihA"
                     /locus_tag="AC06IO_002010"
     CDS             complement(8219..8821)
                     /gene="yihA"
                     /locus_tag="AC06IO_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIO9875186.1"
                     /translation="MPINLYSKAVFLKSAARVNQLPEDSGYEVAFAGRSNAGKSSALN
                     CLTNNKNLARTSKTPGRTQLINLFSLDEQRRLVDLPGYGYAKVAMEVKLEWQKNLAHY
                     LEARQCLRGLILLMDVRHPLKDLDQILVNWALHRELPVHILLTKADKLSRSEVKNAVL
                     KVRQYYELAEHLVSVQAFSSVKKDGVEELISVLDRWYEWN"
     gene            8896..9498
                     /locus_tag="AC06IO_002011"
     CDS             8896..9498
                     /locus_tag="AC06IO_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIO9875187.1"
                     /translation="MKKLALVLILCSSSVIFAQENPQEAGQNKSTVCTACHGPQGIST
                     NPEWPNLAGQHEKYFVKQLKDIKEGKSRSAPTMTAIVANLNEQDMDDLAAYYAKMPVA
                     EGSTPEKYLKRGEQLYRGGDLNKHIAACIACHGPKGSGNAQAGFPLLSGQHAAYTIMQ
                     LQAFKDGKRTNDLNQIMQDISSKMSPEDMEAVAYYIQGLY"
     gene            9509..10123
                     /locus_tag="AC06IO_002012"
     CDS             9509..10123
                     /locus_tag="AC06IO_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIO9875188.1"
                     /translation="MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPL
                     ITEFFSYGCPWCYKIDAPLNDWATRMGKGAHLERIPVVFKPNWDLYAKAYYTAKTLAM
                     SDKMNPILFKAIQEDKNPLATKQSMVDFFVAHGVDREIAKSAFENSPAIDMRVNSGMS
                     LMAHYQINAVPAFVVNNKYKTDLQMAGSEERLFEILNYLVRKSA"
     gene            complement(10200..11498)
                     /locus_tag="AC06IO_002013"
     CDS             complement(10200..11498)
                     /locus_tag="AC06IO_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HIO9875189.1"
                     /translation="MPETIINIENLSKSFKKAPSQHLLVLEDVNFKLQEGEIVALLGK
                     SGSGKSTLLRIIAGLIAPSSGTVTYRGKPVTRPVEGIAMVFQSFALMPWLTVLENVEL
                     GLEAQGISREERRHRAIEAIDIIGLDGFESAFPKELSGGMRQRVGFARALVINPDVLL
                     MDEPFSALDVLTAENLKSDLLELWKEKKTNTNGILLVTHNIEEAATLADRIVIFGNDP
                     GYIRAELPVTLPQPRDPESPEYLALVDKIYTLMTTGPKEKAKRAQRERQIGLGYRLPD
                     VEPSELSGLIETMKSFEERIDLPELADELMMNIDDLFPILETLEILGFAKVSAGDIQL
                     SELGKQFSEADLQERKQLFAQRLLEKVPLARYIRRVLDEKAGHRVSEERFLSKLEDYL
                     SEKEADRVLKTMIDWGRYAEIFAYDFNTGILSLENPGKGA"
     gene            complement(11506..13245)
                     /locus_tag="AC06IO_002014"
     CDS             complement(11506..13245)
                     /locus_tag="AC06IO_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9875190.1"
                     /translation="MGNSRFYFANPDKFSRFINRWDLLLLILTFSVLFFLGWAGSQMA
                     TPYQLGDQIPISLEPSNLPFYALRTVLRMFIALIFSILFTFIVGALAAKNRRAEQIVI
                     PAIDILQSIPVLSFLSITVTGFIHLFPNSLLGPECASIFAIFSAQVWNMTFGFYQSLK
                     TVPHDLIEVSAMFRLSAWQRFWKVEVPFSMSGLLWNMMVSMSASWFFVVLSEAISVAH
                     QNIRLPGVGSYIALAIAQRDLHAVGYAILTMVIVIFLYDQILFRPLIAWSEKFKMEQS
                     PDESEYQSWLIDLIRSSRLMKRVTEWLAVFTNSFVNARWLRIGEVKAVKEIDFKRQKR
                     LDRLWTALVLIAVITGGWFLLKFILAELKVSDIFHVFLLGAATGARVIILILLSSMLW
                     IPVGVWIGLRPRLAQKIQPIIQFVAAFPANLFYPLFVIAIVKFNLSVEIWVTPLMILG
                     TQWYILFNVIAGASSIPRDLYLAADNFGLKGWIWWKRLALPGIFPFYITGAITAAGGA
                     WNASIVAEYVSWGNITLKATGLGEYIQASTTAGDFPQIALGTAMMCVYVLAFNHLIWR
                     PLYRLAEERFNFN"
BASE COUNT         4082 a         2819 c         2613 g         3838 t
ORIGIN      
        1 attaacaatt gaattatata tttaccctaa gatatcagga agttaattgc cttaatagca
       61 ttacttcatg ctaaactagt ttttttggag gaatagtaaa aggatagcta tgaaaagtat
      121 agaggatttt acaggttttt cagaatccgg gattgatgat gccattcaaa acgctttaaa
      181 aaaagcaggt aatccaactc attttgaagt cgttgaaaca ctaggttcac aagatagcca
      241 atcagatcga cgatatcatg taacgcttaa aacactgagt gattaatcat taagtaacgg
      301 caatcatcgg ctagaaataa atgacaaaaa tttaatgtca attctgtttc tataactatt
      361 atctagctgc tgcctttcaa tacaaggagt ttaaaaatgg gatacaatgt gcctcactat
      421 attggtggac aacttattaa tgaaacggat actgaagctc aagccattta taatcctgct
      481 ctgggtgaaa ttattggcca agtccatttt gcgtcaaagg caacctgtga tagagctgtg
      541 gctattgcca aagcagcagg aaatgaatgg tcgcaaaccc ctgctataaa aagagcgcga
      601 gttttattcc gatttagaga gttgctggaa aaataccaaa tggatctggc acgaatagtc
      661 actcgtgagc acggcaaaac gctggatgat gcgaaaggat cggtggccag aggcattgag
      721 gtagtggagc tgcactgcgg gctcgtcaac gaactaaagg gagatttttc tgctgaagtg
      781 gccagccata ttgattgcca tacttttcga caacccttgg gagtatgtgc cggaatatcc
      841 ccatttaact ttcctgtcat ggtacccatt tggatgatga ttcctgccat tgcttgtgga
      901 aataccttta ttcttaaacc ctccgagcaa gatccctctg cgcctgtccg tttattggaa
      961 ctgttaagtg atgctggttt gcctccgggc gttgccaatt gtatccaggg taataaaaca
     1021 actgtcgagc atttactggc tcatccggat atagctgcct ttactgcggt cgcctccact
     1081 cctgttgccg aatacatcta taccactgca gcagcctatg gaaagcgggc acatactttt
     1141 ggcggagcta aaaatcactg tgtggttatg cctgatgccg atttagacca ggcagctaat
     1201 gctatcgtcg gagcagccta tggttcggca ggagaacgtt gtatggcttt atctgtcgtt
     1261 gtaacggttg gtcaacatac cgctgatgcc ttgatcaata aaatttcccc cctcattcga
     1321 gccattcgcg tagacgccgg agatgttgag ggcactgata tgggtcctct tgtcagccag
     1381 gctcatcgac aaagagtttt ggctgctatt gaaaaagggg ttaatgaagg tgcccgcttg
     1441 attatcgatg gcagaacttt tcaacatcct caatacccac agggttttta cctgggaccc
     1501 tgcttgtttg atgaagtaac cgaagaaatg tctgtctatc aaaatgaaat ttttggacct
     1561 gtactggtta tcgtgcgagt caaccatttt gaagaggctc ttgctttagt caataaacat
     1621 caatacggaa atggcactgc tattttcact cgggatggct ttacagcacg tgagtatagc
     1681 cagcgagttc aagcaggcct ggtcggcatt aatattccaa tacctgttcc cattgccaac
     1741 catccattcg gaggctggaa gcgctctgtg tttggagata ccaacatgca cggagaagaa
     1801 agtattcatt tctatactcg acgcaaaacg gttaccagca aatggccaac aaccgaatta
     1861 aaggatagca gctttgccat gcccactcat gattaatcat gcttattacg gagaaatata
     1921 tggctaatat tggatttgtg ggattgggtc atatgggttt tccaatggcc attaatcttt
     1981 tgaaagccgg tcatagtgtc atgggctatg atttgcaaca atcagcactt gagcattttg
     2041 ctgaacaagg aggagctatt gctcataact tgcaggaatt ggcaaaaggc aaagatgtca
     2101 ttgttactat gttacaaacc ggacagcaag ttttacatgt atgccttggt gtagatggta
     2161 tattcagtca gttaaatgct ggttcattgt acatcgattg ctctacaatt gatgtaaaaa
     2221 gctctcgaga aactcatcaa atagcgaaag aaaatcatct tctggtcgtc gatgctcccg
     2281 tctctggcgg agtagcaggg gccgctgcag ccacattaac attcatggtt ggtggtgaag
     2341 cggaggatta taatgcggcg catcccattc tctctgccat gggaaaaaaa atcatccata
     2401 caggcggtgc aggcagtggg caagcagcca aaatttgcaa taacatgata ttgggcacca
     2461 cgatgatcgc catatcagaa gcattcattc tggcaaaaga actagggctg tctgatggaa
     2521 aattatttga ggtggtaaac aattcatcgg gtcaatgttg ggctatgagc aaatacgtcc
     2581 ctgtaccagg aatagtggaa aatgttcctg caaataatga ttataagcca gggtttgcag
     2641 caaacatgat gttgaaggat ttgttattga gtcaaaatac tgcgcaatca gtacacattg
     2701 aaacacccct tggcgccaaa acaactgaaa tttatcaaca atttgtcgat caaggccttg
     2761 gtgatatcga tttttcagcc attattaaac acattggcaa aacacaagag gtgtagcatg
     2821 cttcccgttc atctaaagca ccctcattta acacctacag aagaatctct atctgaattt
     2881 tggtctgaaa ttgccgcaca aaccattgac tggatacaac cttggagtat caccctgcag
     2941 ggagaattac acaaaggcga tgtgaaatgg tttcaaggcg gattgctcaa tgttagcgct
     3001 aattgtctgg ataggcattt accccataaa gcaaatcaaa cagccatcat ttgggaaggt
     3061 gatgatgaaa accaaaataa aacccttact tttgcgcagc tttattctga agtatgcaaa
     3121 atgagcaatg tgttaaaaag cctaaatgtc cgcaaaggag atacggtagg catttaccta
     3181 cccatgattc ctgaagcggc cattgccatg ctggcttgtg ctcgtatagg agctattcat
     3241 accgttgtgt ttgccgggtt ttcggcccat gcattgcagc agcgattaat agcctcttcc
     3301 tgtaaatgtt taatcactgc agatgcgttt caacgcggtg gaaaaactat ccctttaaag
     3361 aaacaggctg acgaagccag cgtggactta aatatcacca agctggttgt taaaaattca
     3421 aatgccccaa cggcactaaa taagaacaaa gaacattggt ggcatgagct aaaacaaacc
     3481 gtatcggatc aatgcacgcc ggaaccaatg aacgccgaag atcccctgtt tattctctat
     3541 acttccggaa gcacgggaca gccgaaagga gtggtacata ccacaggcgg ctatttagtg
     3601 cagaccgctt atacgcatca actgatattt gcttgtcagg ataatgaagt attttggtgt
     3661 actgctgatg tcggttggat tacagggcat agttatgtcg tctatggccc tctctgcaat
     3721 ggcattacca cattgatgtt tgaaggaata cccacgtggc ctgacgcagc aagaaactgg
     3781 cgcatcattg ataagcacca agtgaatgtg ttctacacag ctcccacggc catccgctcc
     3841 ctgatgagag ctggcgatca atggctaaac tcaagctcaa gaagttctct tcgtttactg
     3901 ggttcagtgg gtgagccaat taacccggaa gcatggaatt ggtatcacca aaaagttgga
     3961 caagggaaat gcccgatagt ggatacatgg tggcaaactg aaactggagc cattatgatt
     4021 agtcccagag ccagtgatgg agtgatcaaa ccaggctctg cttgtaaacc tattccgggg
     4081 atcgtaccac tcttgttaaa tgagcaagga catgaaatta atggcgctgg agaaggcctt
     4141 ttggctatca aatacccatg gccttctatg gcaagaacca ttgcaggaga tcaccaaaga
     4201 tactgtaaca cttatttatc caacggctat tacatcactg gtgatggggc caaacgagat
     4261 gatgatggag attattggat tacaggccga attgatgacg tacttaatgt ttctggccat
     4321 cgattgggta cggctgaaat tgaatcagca ttggtcagcc atcctaaggt tgctgaagct
     4381 ggcgttgtag gtattcctca tgatttaaaa gggcaaggaa tatttgccta tgtcattctg
     4441 aagcagggaa ataagccgga tgccaagctc caaactgaat tgatggagcg agtcaaagat
     4501 gaaatcagtg ctatcgccaa accagatgtc attcagtttg taaatgattt gccgaaaacc
     4561 cgttcaggta aaattatgcg ccgaatacta agaaaaattg cttgtaaaga agtgtcgcac
     4621 attgatgagc tgggtgattt aacaactctt gctaaccctc agatcgttga ggaactcatg
     4681 aaaaatattt tgaagcggta aaagaaccgt cgagcttcac aaagtttatt ctgatagcaa
     4741 acaaataaac tatgtgaagc ctgacaacca gtccaaataa tatcaaatca atttcatcag
     4801 gctctgacag cgagcggttg cattgacaat gaaaaatctt tgacaatttt ttcagactca
     4861 gtgggtacag gaaataatcg ccatctcccc gcgtataaat tggctccgcc caaagtgcat
     4921 aaagtgacta aagcactcgc taaatcagca aatacttgtt tccagccacg atgtacttct
     4981 aaaacaggta tgactgcatt aatggcacgg acgcacgctt gatgaaaatc attgcatttc
     5041 acattcttgg gcagttgaga gtttttgaag tgctcttcgg cccttaataa attattgtaa
     5101 aatgttctcg ctatgggtgc cgcgcgttta taattttcat cgctaaccgc tttggtttcg
     5161 agcgttttaa ccatactttc aatagttttc aagtgatcgg aaaatttaat ttcggcaaga
     5221 atattcaagg tttcttttat tgcagcgatt tggtcagtaa tacgcttgct cattacatcg
     5281 gcttgtctgt taatttcaca aattttatct gtcagagcta ttttgatatc actatgatac
     5341 tcatcagata gttgaaggtg ttcctgtgcc ttaagtaaat ctggtttgtt atccaccaaa
     5401 tatcgaaccg attgaattaa atcctgtttt ctggcaatca cttcttcaac tgtttggcac
     5461 ctgctaaaac ttatcggaaa ttccctgatt tgtttttcat agctttttac tacttcttca
     5521 gcatttattt taatgatcaa ttgatcagct gtttcattta tttcttgttt tttcgcttgc
     5581 agtgctctta ctatggatgg ttgcaagtca ttacaatcca gaaccttttc agaacttaac
     5641 aaagcttgat taggtttaac cagattatca agttgttgca ataaattatt acgctgccat
     5701 tctactgcag aagtggagct tatatcatta aagcgaatta caaaattatc aattcttttt
     5761 tcaacagaat cgataacctt ttgcgcctca tcaagaagcc gtttttgcaa taattcagcc
     5821 cgcaaattaa tcttttcaac ctgggcataa tagaccattt ttatttttgg attggcgatg
     5881 aattgttcgc ccaaaagatc ttccgctttg acctgtgtgt gaagcttttt gttcaacaga
     5941 atacgtcggg attcaatagc tactatggta tccgcatcat taaagtcgat tgggaaattt
     6001 tttatttgcc tgacatgctc atctatatcc gtttcatact tcctgagaaa atctgaacgt
     6061 tgattagctg cggcgtctat cattcggagc ttacgttcta aagccctctg gacttccgga
     6121 tgtccatcag ggaaatccag tatttgcaac gcattttgca attcgccaga atgaactatt
     6181 ttcttaagtt ggcctctgag ctcttctgca ttaagattaa cgtgttctac ggttttaaat
     6241 ccctcaaaac taaatggaaa agaattgatt cgctgcacaa tatctgaaat cactctttta
     6301 gcatcgtcga ctcgttgttg ccttaataca ttatcaattt ctggaatttt tatcacaggt
     6361 tgtggtttgg ataaggactc aaaccatgat ttgaattcgc ctgaatcttt taaagcgatt
     6421 ttttttgcgt attcttctaa agatatgcta aatagaacat taattttttg agatgcgaag
     6481 acaaataaag cttttgccac ttctggatta attcggctta atctgaacaa actttcaaag
     6541 aattcatccg gatctttaaa cgttgcgctg gcaagaacga catcaaaaaa ctcctcatca
     6601 attgacgatg tgtctttgct ttttaaatac ttaatcaata aatccggtaa agtctttagt
     6661 gtgagatagt tagcaatcgc ttttgcaaaa agagcttttg gcggaaataa tgcctctatt
     6721 gattttttgg agaaaacatt ggctataaac aatgctcgta accctgatgc tcttgttaaa
     6781 ctagaagatg atccaccaaa gtaaaagtta attaacccgg attcaatttc ttgtttcgct
     6841 ttttcaggca tgtaagctaa ctgattgatt ttttgctgta ttgcagactt cactattaaa
     6901 tggagaaaag cctcgccatt aaaatttatg cctgctttgg gacttaattt gttttcttcg
     6961 actgggcttg acaaggctaa ctctgtcaaa acctcgccta ataattcttg agatgtttct
     7021 tcacgcagaa ccagttcaag caaatccatt gcagggatac gggttaaaaa taattttagg
     7081 aattcttttt tagaaccatc cgcattgact atgcaagtag ttaatgtttt cttttcctcc
     7141 actgacaatt ttaggaaaag tttttcaaga taagggattt gtttatgcaa actaatgcaa
     7201 gaagaaataa ttttgttgtc agttaaaaga ttcatttcct ccaaagatat cagccttttc
     7261 tgcagaccag aattggcaaa aacatacttc ccctcctcgt tggaaagagt caataatttt
     7321 tttgtcaaac tggctctatg gatactggta ttaaaaaggc aaaatcggca gtttcgtttg
     7381 ttgagatcgt ctaatttgta agtttctgtt ttagaattat cgtgcggatt gattaaaaca
     7441 aaatcatagt ttccagaacc tttaggaata attttgtcta ttctcaaagc atgacggcca
     7501 tgaaattttc caaagctatc gacttttcca taagacatac taatataaac aggttcatca
     7561 ggattcatta atttcaattt gatgatatcg tcaagctttg aatatggaat gtcttccgct
     7621 tgaataccaa agaatttgcc taaaaaagca gtggaagtaa aaccggcaat tctgtccggt
     7681 atatcatgag caacaacgct tgccaaagga tcagtattac tccaatctcc cgcataataa
     7741 taggaaacca atcgttccaa aatttttatt gccagtgaat tactttttac ccctccgtat
     7801 tgattatcaa tttcctttag tctttccggg ctaatctcaa acacatcttc attgttgagt
     7861 tcatccacgt aatgggtgta cttcccagtc attttattct tttgcaaatt atcttttagt
     7921 gcctcatggc gtttgattct tacgataact ttcccatctt ccgtttgggt aaacaacgat
     7981 ttaattaatt gctccccatc cgctccaaga tttaaaatgc aatccagaga tgacaaaaga
     8041 tagcaactac ctccaatacc ttgtcggatg gtaatagggc cttgggttgg gaacaaaggt
     8101 gtcattgtgg tcctcttaaa atcataacgt acagaaagag ggcattataa cagcaaaata
     8161 ttggataatc attaaaaaaa atggtctgta atcaatcgga aaaccaaagc cctaaaagct
     8221 aattccactc ataccaacgg tcaagcacgg aaatcaactc ctcaacacca tcctttttca
     8281 cagaagaaaa cgcttgcaca gaaaccaaat gctctgccag ctcataatac tgccgaactt
     8341 taagtacggc atttttgact tcactacgac ttaacttatc tgcttttgtc agtaaaatat
     8401 gcactgggag ctctctgtgc aaagcccaat tcactaatat ttgatccaaa tctttcaatg
     8461 gatggcgtac atccatcaac aaaattaatc ctctcaaaca ttgccttgct tccagatagt
     8521 gggctaaatt cttttgccat tccaatttta cttccattgc taccttggca taaccataac
     8581 ctggtaaatc aacaaggcgc ctttgctcat caagagaaaa gagattgatt agttgagtgc
     8641 ggcctggtgt tttgctggtt cttgccaaat ttttattatt agtcagacaa tttaaggcac
     8701 tggattttcc agcgttggaa cggcctgcaa acgctacctc atacccggaa tcttcaggta
     8761 attgattgac acgggctgca ctttttaaaa atactgcttt agaatataaa ttgattggca
     8821 taagatatca ttttagtttt gggattttga cccaggcagt gtaccattaa ttgactaaat
     8881 tattatgaga aatccatgaa aaaattagca ctcgtattaa tcctgtgctc ttcatcagtg
     8941 atttttgccc aggaaaaccc gcaagaggct ggacaaaata agtcaacagt ctgtaccgcc
     9001 tgtcatggtc cgcaaggtat tagtacgaat cccgaatggc ccaatcttgc cggccagcat
     9061 gaaaaatatt ttgtgaaaca attgaaagat ataaaagaag gtaagtcgcg ctctgcgccc
     9121 actatgactg caatagttgc taatctgaat gagcaggata tggatgactt ggcagcctat
     9181 tatgctaaaa tgcccgttgc tgaaggttca acccctgaaa aatatttaaa aagaggcgaa
     9241 caactttaca gaggtggtga tttaaacaaa cacatcgcgg cctgtatagc ctgccatggc
     9301 cctaaaggaa gtggaaacgc tcaagcaggc tttcctctgc tttctggtca gcatgcagct
     9361 tatactataa tgcaactgca agcatttaaa gacgggaagc gaacgaatga cttaaatcaa
     9421 attatgcaag atatcagtag taaaatgagc ccagaagata tggaggccgt tgcttattat
     9481 attcaagggt tatattgagg aattgactat gtttaaaaaa ttaattgccc tattatttct
     9541 tatgccaatg actgctttgg caactcaatt tattgaaggc aaagattatc aaaccgttgc
     9601 cagcgcgcaa ttgtctacca ataaagataa aacacccctg attaccgaat tttttagcta
     9661 tggctgtcca tggtgttata aaattgacgc cccactaaat gattgggcaa ccagaatggg
     9721 taaaggagca catcttgagc gtattccagt tgtttttaaa ccaaactggg atttgtatgc
     9781 caaagcctat tatacagcaa aaaccctggc tatgtccgat aaaatgaatc ccatattatt
     9841 caaagcgatt caggaagata aaaatccact ggcaacgaaa caatcgatgg ttgatttttt
     9901 tgttgcccat ggcgtagaca gagaaattgc caagagcgct tttgaaaatt cgccagcaat
     9961 cgatatgcgc gttaacagcg gcatgtcttt gatggcgcat tatcaaatta acgccgtacc
    10021 agcctttgtc gtcaataaca agtacaaaac tgacttacaa atggcgggga gcgaagagcg
    10081 tttatttgaa attttgaatt acctggtaag aaaatcagcc taattaactc aagctataaa
    10141 ggatattcct cgccacggta attgaccact ggccaggtga ttttgacctg gctttcttgc
    10201 taagccccct tgcccgggtt ttccagactc aaaataccgg tattgaaatc ataagcaaaa
    10261 atctcggcat atcgtcccca gtcaatcatt gttttcaaaa cacgatctgc ttctttctcg
    10321 cttaaataat cctccagctt actcaaaaag cgctcttccg agacgcgatg ccccgctttc
    10381 tcatccagta cgcgtcttat ataacgagcc agagggactt tttccaataa tctttgcgcg
    10441 aacaattgct tacgctcttg caaatcagct tctgaaaatt gttttcctaa ttcacttaac
    10501 tgaatatctc ccgcagacac cttggcaaaa ccaagtattt ccagagtttc aagaattggg
    10561 aacagatcat caatattcat catgagctcg tctgccagct caggtaaatc aatacgttct
    10621 tcaaaggact tcatagtttc tatcaaaccg gataattcag agggctcgac atcaggtaag
    10681 cgatagccta atcctatttg acgctccctt tgcgctcgct tggctttttc tttgggtcca
    10741 gtagtcatta aagtataaat cttgtcaacc aaggcaagat attccgggct ttccgggtca
    10801 cggggttgcg gaagagtcac tggtaattca gcacgaatat agccgggatc attaccaaaa
    10861 atcactatcc tgtcagccaa agtcgccgct tcttctatat tgtgtgttac taataaaata
    10921 ccattggtat tcgttttctt ttctttccat aattccaata aatctgattt gaggttttct
    10981 gcagtaagta catcaagcgc ggaaaagggt tcatccatta acaacacatc cggattaata
    11041 accaaagcgc gagcaaaacc aacccgttgt cgcatcccgc cagataactc cttgggaaaa
    11101 gccgattcga aaccatccaa acctataata tctatcgctt caatagccct gtgccttctt
    11161 tcctcgcggc tgattccttg tgcttccaaa ccaagctcca cattttccag gactgtaagc
    11221 caaggcatta aagcaaacga ctgaaatacc atagcaatac cttctaccgg cctggttaca
    11281 ggtttaccgc ggtaagtcac tgttccactg gaaggagcga taagaccggc aataatcctg
    11341 agtaaagtgg atttccctga accagattta cctaacaaag caactatttc gccttcctgt
    11401 aatttgaaat taacatcttc aagtacaaga aggtgctgtg aaggtgcttt tttaaaagat
    11461 ttgcttaaat tttctatgtt aataattgtt tccggcatag gagtctcaat taaaattaaa
    11521 tcgttcttcg gctaatcgat aaagcgggcg ccaaataaga tggttgaaag ctaatacata
    11581 gacacacatc attgctgtgc ctaacgcaat ttgcggaaag tctcccgctg tcgtactagc
    11641 ctgaatatac tcccccagac ctgtggcctt aagagtaata tttccccagc tcacgtattc
    11701 agcgacgata ctggcattcc atgcaccgcc tgctgctgta atagctccgg taatgtaaaa
    11761 aggaaaaata cctggcaaag ccaatctttt ccaccatatc caacccttta acccaaaatt
    11821 atcagccgcc agataaagat cgcggggtat actcgaggct cctgcgatca cattaaataa
    11881 aatataccac tgtgtgccca ggatcatgag aggagttacc cagatttcca cgctcaaatt
    11941 aaatttgacg atagcaatta caaataaagg ataaaacagg ttcgctggaa acgctgccac
    12001 aaattgaata atcggttgta ttttttgagc cagccttggc cttaatccta tccacacccc
    12061 aacaggaatc catagcatag agcttagcaa aattaatata atgacccgag ctcctgttgc
    12121 tgcgcccaat aaaaagacat gaaatatatc actcaccttc aattcagcga gaataaattt
    12181 caacaaaaac cacccgccag taataacggc tatcaaaacc aacgctgtcc acaatcggtc
    12241 caaacgcttt tgccttttaa aatcaatttc cttaacagcc ttaacctcac caatcctcag
    12301 ccaacgagca tttacaaaac tattggtaaa gaccgcgagc cattccgtca ctctcttcat
    12361 caaacgacta ctgcgtatca agtcaattaa ccaggattga tattctgact catcgggaga
    12421 ctgttccatt ttaaattttt cagaccaggc aataagtggc ctgaaaagta tttgatcata
    12481 caaaaaaata acgataacca tggtcagaat ggcatatccc acagcatgca aatccctctg
    12541 ggctatcgcc aatgcgatat aagaacctac accgggtaat ctgatatttt ggtgcgccac
    12601 cgaaatagct tctgacaaaa ccacaaaaaa ccagcttgcc gacattgaaa ccatcatatt
    12661 ccataacagt ccagacatgg aaaaaggaac ttctaccttc caaaagcgct gccaggcaga
    12721 caacctgaac atggcagaga cttcaatcaa atcatgagga actgttttta aagattgata
    12781 aaatccaaaa gtcatattcc acacttgggc gctaaaaata gcaaaaattg aggcgcattc
    12841 tgggcctaac aagctattgg gaaataaatg aataaacccg gtgacagtaa tagataaaaa
    12901 actcagaaca ggaatcgatt gtaaaatatc aatcgccggt ataacaattt gctcagccct
    12961 gcgatttttg gcagctaaag cacctacaat aaaagtaaac agaattgaaa aaatcaatgc
    13021 gataaacatg cgtaaaactg ttcgtaacgc ataaaaggga aggttagaag gttccaaaga
    13081 aatgggaatt tgatccccta attgatacgg ggtagccatc tgtgaaccgg cccaacccag
    13141 gaaaaataac acagaaaaag tcaatataag caatagcaaa tcccatcgat taataaatcg
    13201 actgaattta tcaggattag caaaataaaa ccgactattg cccactatac cctctcttaa
    13261 aattgacgtt acaagtttcc cttaatctaa ttccttaaat ctaaaaaata tttatccgtt
    13321 cctgaatcag gatttacgaa aaaccccaag gt
//
LOCUS       DBNCWK010000084        13251 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.084, whole genome shotgun sequence.
ACCESSION   DBNCWK010000084 DBNCWK010000000
VERSION     DBNCWK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13251
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.084"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            13..726
                     /locus_tag="AC06IO_002015"
     CDS             13..726
                     /locus_tag="AC06IO_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6969 family protein"
                     /protein_id="HIO9875191.1"
                     /translation="MNSQSDFNLPILSSWYKKKFLGYASQVLEAQQKMTSSKGKNILH
                     YTLRKKRKHERMSHYPKGDRIDRSTGSQYFYHCHRENFESNEHGHFHCFLRYKHIPKR
                     IKPAPLEDWDKYIDNPMTHLVAIGMNQFGQPIRLFTVNRWVTSEIWYGAEHIPYFLKS
                     YKMTLIDDPYWQVLDQWVEGMLHLFAPQIAWLHQERDKRIQLHQLNSPNDNPYTNHEL
                     EELSEINIDLKKQIEWVIS"
     gene            879..3221
                     /gene="purL"
                     /locus_tag="AC06IO_002016"
     CDS             879..3221
                     /gene="purL"
                     /locus_tag="AC06IO_002016"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIO9875192.1"
                     /translation="MQNLTETLDFSSLNREEIEKLLRDYNLNLSVDEALTIQNEFLKR
                     PPTISECVLWSIQGSEHCSYKSSRIHLKQFNTSGPHVILGAKEDAGIVSVAQDKNGYR
                     YGVIVSHESHNHPSQIVPYEGAATGVGGNVRDVCCMGGEVIAVADSLRFGDIKRARTH
                     WIQEGVVSGIAGYGNPLGIPNIAGDVYYDPAYNENCLVTVVTLGIVREDHIIHSYAPP
                     EADNYVFILVGKPTDNSGFGGASFASTVLEEDSQEKNKGAVQEPNAFLQRHLLKANYS
                     LFQLLREKNLIDRVGFKDLGAGGVACASIELAEAGGSYGAEIDLDKVPTGMPGLMPSV
                     ILCSETQERFMWVVPPDLVDTILKHYNETFALPQVSEGARAAVIGKIRTDGLYVVNYK
                     GRELVRAKVPDVTKGIVYNRPHASSQKQNTEPVFLPPDDYNQILLQLLAHENIASKEP
                     IFEMYDKQVQGRTLIQAGWADAGVLQPFNESKYPEEIRKTGIALSLDQNPRYNKIDAY
                     WGAVNAVAESVRNITAVGATPVAITDCLCFGNPEKPEQMREFVDSVRGISDACAAIHL
                     KDHPQSTLPVIAGNVSLYNESVKGAIPPSPMISCLGTLPDIDFAITFDFKKSDSLLIL
                     IGERKDECGGSVYYQLHNQLGSNVPKPDLSLFNREIHAVSSAIQHGLVNAAHDVSEGG
                     VAVALAEMSFKNSLGVAVQINGELSTDKLLFGETGGFILEIDKQHKAAFDKLVTQYQV
                     PYMVIGHTTEQPVLQMNSVINLPVEEARQAWENGLRERLL"
     gene            3221..4264
                     /gene="purM"
                     /locus_tag="AC06IO_002017"
     CDS             3221..4264
                     /gene="purM"
                     /locus_tag="AC06IO_002017"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIO9875193.1"
                     /translation="MSTINYKSAGVDIEAGNEAVARIKRNVEKTFSPQVLTGIGSFGA
                     MYDLKPFMEKFKHPVMVQSIDGVGTKTIVARMMQKFDTIGIDLVSATTNDIIVLGARP
                     LTLLDYIATDRLKPEYIEEIIIGMTQACLENNISLVGGETAEMPDTYLKGEHDLVGII
                     TGVVEKDEAILGKDITPGDSVLAFPSSGLHTNGYSLARKLLFELGGYTVESRLDELDA
                     AVGEALLVPHINYTNPVLHLLNNKIPIKGMAHITGGGVLENIPRVLPNYCSVEINKNS
                     CPVLPIFNVLKALGKLDDQEMYRTFNMGIGLVMIASPGVVSEIHTALKEFPNFPVYEV
                     GQVVSGKPEVRLL"
     gene            4264..5523
                     /gene="purQ"
                     /locus_tag="AC06IO_002018"
     CDS             4264..5523
                     /gene="purQ"
                     /locus_tag="AC06IO_002018"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HIO9875194.1"
                     /translation="MKIAVVQFPGSNCERETMLAVQRAGMTPVEFLWNDSSQKLREMD
                     GYIIIGGFSYEDRSRAGIIAALDPVMKEIKLQSELGKPVLGICNGAQILVETGLVPGL
                     KNYQIGMALTENKRIQDGKILGTGFYNSWIHMRTGDRVRQNAFTRYLSKDSVLSIPAA
                     HAEGRFVMPFKLLQEIEEEGLNAFQYCDAQGNRDDNFPVNPNGSLRNIAAIINKAGNV
                     MAIMPHPERTFAGDPIFHSMKEYIKEKKTFKQQTLSYQPEPVKIIPYDKKDNAHELIV
                     KLIITDNAALTVQNTLRQLGIPVTVNRMMCWHVDTNFPEAIEKIKQTGVLYNDRKEYL
                     VTPEQIFSSAAKAFLVKSKDDMIGQQKLQMLRDHFSIDGVDAIHHGVLWIFSSENVKI
                     QELAESILATNIIFNPNAHICYEYQCP"
     gene            5504..6484
                     /locus_tag="AC06IO_002019"
     CDS             5504..6484
                     /locus_tag="AC06IO_002019"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIO9875195.1"
                     /translation="MNTNVPSQEDLLAALPFCLTETSLPFGKKYKGKVRDTYDLGDQL
                     ILVTTDRQSAFDRCLAAVPYKGQVLNLTSVWWFKNTQSIVPNHLIAVPDPNVAIAKKC
                     KIFPVEFVVRGYISGSTSTSLWTQYQKGVREYCGITFPDGLRKNQKLESPVITPTTKE
                     TIHDRPISPHEIVAEGWMTQEDWDETSSYALRLFQHGMEVAQQHGLILVDTKYEFGRD
                     AEGRIVLVDEIHTPDSSRYWLFNSYQERFDAGKEPENIDKEFLRLWFVDHCDPYKDEV
                     LPQAPQELIVTLASRYIQLYEMITGESFVYDSNPGPVNDRILHNIQRWLG"
     gene            6507..8006
                     /gene="purF"
                     /locus_tag="AC06IO_002020"
     CDS             6507..8006
                     /gene="purF"
                     /locus_tag="AC06IO_002020"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIO9875196.1"
                     /translation="MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFK
                     HGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGN
                     LVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
                     EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHEDGSKDYIFASETTPFYA
                     LGFEPKGDLLPGEVAYVNQQGQLYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRA
                     RLRMGQNLAKSWKKRFPDTLPDVVIPAPSTANTAALAFANELGIRYSEGLYKNPFIGR
                     TFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEI
                     YFVSTCPPIKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQESLIEAVSR
                     RGQYDMSKPCMACMNGKYICGQITEEKIKLLEKKRLTENDQETEIGEMS"
     gene            8003..9322
                     /gene="purD"
                     /locus_tag="AC06IO_002021"
     CDS             8003..9322
                     /gene="purD"
                     /locus_tag="AC06IO_002021"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIO9875197.1"
                     /translation="MINILIVGSGAREHAIATALSKSENKPKIYCFGTTRNPGIQRLT
                     SDYWVGDITDPEQVNQRAKKWEINLAIIGPEAPLEHAVADALWDCGIPVIGPRKNLAK
                     IETSKAFARELMEKYGIPGLPKYKVFTSLEGIGEFLSELGENNFVVKANGLMAGKGVK
                     VGGEHLLSFEEALNYCKEIFTHNQSLVIEEKLIGQEFSFMCFSDGISLVPMPIVQDHK
                     RAYEHDEGPNTGGMGSYSAADHSLPFLSPEDVRAAFAINQSVINALMTEMSDKYIGIL
                     YGGFMATAHGVYVIEFNARFGDPEALNVLSLLESDFVKLCIAMANGNLQKESVRFLNQ
                     ATVCKYAVPDGYPDNPMRHVEIDISAVEPKDTLYLAAVNFKDGKLYATGSRTAAYVGI
                     ANTISEAEEIAEQQIKNIKGPLFHRKDIGTSPLINTRVEVMRRLRAL"
     gene            9319..9897
                     /gene="purN"
                     /locus_tag="AC06IO_002022"
     CDS             9319..9897
                     /gene="purN"
                     /locus_tag="AC06IO_002022"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIO9875198.1"
                     /translation="MIRLGILGSTRGTNMLALVDAINEGTLKAKIELVISNKPDAIIL
                     ERAKSLGLNAQFVNPEGLNRIDFDKKVSDILINHQIDLIVLIGYMRILSADFVNKWNN
                     QVINVHPSLLPAFAGKMDIDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVL
                     EGDTAQTLKARVQQLEGMALVAAINLIASKGL"
     gene            complement(9900..12011)
                     /locus_tag="AC06IO_002023"
     CDS             complement(9900..12011)
                     /locus_tag="AC06IO_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HIO9875199.1"
                     /translation="MGYHFLMEGNGIIACYDMYPTQFHSINDMIDYLPVLKQMGFNAL
                     WINPMQMPGDISGFFKTDKNNGVKTGNEVTRSLYAMSHPLLFNPQFSLGSPEDPMETT
                     QRLNSEALQLFTQNARNLGIVPMFDLVLNHVAIDSPLCQDKPHWFKGVHEDFKDVRGF
                     NYDDESIRKEIITEFWQPYIKRYMIEYGFDGVRVDAVGYVHPAVRSEIYAYIHSLAAE
                     NGKPKPVILDEALFSKRPLADEVNYLKLPGIGPTHITTEAYNVEFDLASADLPYEITL
                     EEQLKASVVFQQKDGTWRENTKGGCINFCGNHDYRSLAMTILFQMAKKRLKSDAFYND
                     LMSSYQDLYFKFNAEQEKPHELKEPLKTTLLYSYVDQIKKELEDNQDETAQEFKTLVL
                     EKLALSALTASGGWFLLSGDETCDITAKTVFQRKGAVDKSYYPQREHRIFSEKPVIAH
                     KILEKMAEENFFIENSENKWMQELYRSLSSFPEMQKRLLVSHIDNIKYQINAGIDHVQ
                     EKFSRLLASESLNIGFTSQDFSENPRTHENGWLGLQNNFLFIKQLNTLLKTLPASLPG
                     FSSELVRLTGKPHLIIVVRKNGNELSAPIDIVVVNLEQEKRQTLTKEDFQLIAKSYCN
                     QYFSKNNPSVCDSLREKLYSHVLKSAANNRVHTDTSIKLNGIHLFEFKETSLNTFFTI
                     EKEKNYSHRNEDHTALTGNTI"
     gene            complement(12115..13005)
                     /locus_tag="AC06IO_002024"
     CDS             complement(12115..13005)
                     /locus_tag="AC06IO_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1670 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875200.1"
                     /translation="MGSSYNDWLKSSYSELKKINIIENLIKENNFARAKMLLNNLDLT
                     TLIKYTELSKTITDFCEEAEQADIWRTHLQNFNEEHFSFEEYPPLTVSQLVKGIYFYG
                     QAAECREEEGKPFGDNELEFLKKSAHQHCFYAYNSLSTWAYEKYKMGLNDYSLLTLHY
                     AQKASQYHWTPGYLLFYKTCLNLAILSNAPSLSYQEALEALLIARKLSEHQYSISAIN
                     NAYFGKGLIHGNKTQMESWDKAISETIAKGKIPSALINKIYDKASEKAKQILDEFTHE
                     VKDKPEEEQLDNESAPNLSF"
BASE COUNT         4065 a         2272 c         2787 g         4127 t
ORIGIN      
        1 ttttaagtaa ttatgaatag tcaatcagat tttaacttac ccatattgtc cagctggtat
       61 aaaaagaaat ttcttggtta tgcttcacaa gttcttgaag cccaacaaaa aatgacatcc
      121 agtaaaggta aaaatattct gcactacacg cttagaaaaa agcgaaagca tgaacgcatg
      181 agtcactatc ctaagggaga caggattgat cgtagtactg gctcacaata tttttatcat
      241 tgccatagag aaaatttcga aagcaatgaa catggtcatt ttcattgttt tttgcgatat
      301 aaacatatac cgaagcgtat taaaccagct cctttagagg attgggataa atacatagat
      361 aatcccatga ctcaccttgt tgcgataggt atgaaccagt ttgggcaacc gatacgatta
      421 tttacagtca atcgttgggt gacatcagaa atttggtatg gtgctgagca tattccatat
      481 ttcctgaaat cttacaagat gacattaata gacgatcctt attggcaagt attagatcag
      541 tgggtggaag gcatgctcca tttgtttgct ccccaaattg cctggctgca tcaggagcga
      601 gataagcgca ttcagcttca ccaactgaat agcccaaatg ataatccgta cactaaccat
      661 gagctggaag agctttctga gataaatatt gatttgaaaa agcagattga gtgggtaata
      721 agttaaattc caaaattaga accacaactt aatttatctt aattattggt ttttcattgc
      781 ttataccccg tgtaaaaata tacaatatct attgctttgg ggagagatta gtcctttact
      841 gatcaatcag agattaattc tatctggtat agagattcat gcaaaatctt actgaaaccc
      901 ttgatttttc ttctttgaat cgtgaggaaa ttgaaaaatt actgcgcgat tataatttaa
      961 atctttctgt agatgaggca ttgactatac agaatgaatt cctcaaaaga cccccgacta
     1021 tttcagagtg tgtactttgg tctattcaag gatcagaaca ttgttcttat aaaagcagcc
     1081 gtatccattt aaagcaattc aatacctcag gccctcacgt gatactgggc gccaaggagg
     1141 atgcaggtat agtatctgta gcccaagata aaaatggtta tagatatggc gttattgtca
     1201 gtcatgaatc acataatcac ccatctcaaa ttgttcccta tgaaggcgca gctaccggag
     1261 ttggtggtaa tgtccgtgat gtctgttgta tgggtgggga agttatagca gtagcagaca
     1321 gtttgcgttt cggagatatc aaacgagcac gtactcactg gatacaggaa ggggtggttt
     1381 caggtattgc cgggtatggc aatcctttgg gaataccgaa tatagctggc gatgtgtact
     1441 atgatccggc ctacaatgaa aattgtttgg ttactgtagt cacgttgggg atagtaagag
     1501 aggatcatat catacattct tatgctccac ctgaagccga taattatgtt tttattttag
     1561 taggtaaacc aactgataat agtgggtttg gcggcgcaag ctttgcctca acagtattgg
     1621 aagaggattc acaggaaaaa aataagggcg ctgttcaaga gcccaatgct tttttacaga
     1681 gacatctcct taaagccaat tattcattat ttcaattact aagagaaaag aatttaattg
     1741 acagggtagg ctttaaagat ttaggagcag gtggcgtggc gtgcgcaagt atcgagttgg
     1801 ctgaagccgg cggttcttat ggcgccgaaa ttgatctgga caaggtaccc acaggaatgc
     1861 cagggttaat gccttcagtc attctatgct ctgaaactca ggagcgtttt atgtgggttg
     1921 ttccccctga tttagtagat actattctaa aacattataa tgaaaccttc gcgttaccac
     1981 aggtttctga aggggcacgc gctgcagtta tcggcaaaat tcgtaccgac ggattgtacg
     2041 tggtgaatta caaggggcga gaacttgtga gagctaaagt cccggatgtc acaaaaggca
     2101 ttgtttataa caggccacat gcctcttcgc agaagcaaaa cacagaacca gtttttctcc
     2161 caccagatga ttataatcaa attttgttgc agttattggc ccatgagaat atagcgagca
     2221 aggaacctat ttttgaaatg tatgacaagc aagtgcaggg tcgcactctc atacaagcag
     2281 gatgggccga tgctggagta ttacaaccat ttaatgaatc gaagtatcct gaggaaattc
     2341 gcaaaacagg tattgccttg tctttagatc aaaatcctcg ctataacaaa attgatgctt
     2401 attggggagc tgttaacgcg gttgccgaat cggtacggaa tatcactgca gttggagcta
     2461 ctccagtcgc aataacagat tgcttgtgtt ttggtaatcc ggaaaagccg gaacaaatgc
     2521 gcgaatttgt tgattctgta cgaggaatta gtgatgcctg tgctgctatt catttgaaag
     2581 atcatcccca atcaactttg cctgtcattg ccgggaatgt ctcattgtat aatgaatcag
     2641 taaaaggggc tattcctcca agtccaatga ttagttgcct gggaacgctt ccagatattg
     2701 attttgcaat tacctttgat tttaagaaaa gtgatagcct attgatattg attggagagc
     2761 gtaaagacga atgtggtggt agcgtttatt atcagctcca taatcaattg ggttctaatg
     2821 ttccaaaacc ggatttaagc ttgtttaata gagaaatcca tgctgtttca tcggcaattc
     2881 agcatgggtt ggttaacgct gctcatgatg tctcagaggg aggggttgct gttgctcttg
     2941 ctgagatgag ttttaagaac tccctgggag ttgctgtaca gattaatgga gagttatcca
     3001 ctgacaaact tttatttgga gagacaggcg gttttattct tgaaatcgat aaacaacata
     3061 aagccgcttt tgacaaactg gttactcaat atcaagtccc ttacatggtt atcggacata
     3121 ctacagagca gccagtttta cagatgaatt cggttatcaa cttgcctgtc gaagaagcga
     3181 gacaggcttg ggaaaatggc ttaagagaga ggctattata atgtctacta tcaattacaa
     3241 atcagcgggt gtcgatatcg aggcaggaaa tgaagctgtc gcacgcatta aacgtaatgt
     3301 tgaaaaaaca ttttcgcctc aagtcctgac aggaatcggt agttttggtg caatgtatga
     3361 tttgaagcct tttatggaaa aattcaaaca ccccgtcatg gttcaaagca ttgatggcgt
     3421 aggcacgaag acaatagttg ctcggatgat gcaaaaattt gatacaatcg gaattgattt
     3481 ggtcagtgcg accaccaacg atattattgt gttgggtgca aggccattaa cacttttgga
     3541 ttatattgca actgatcgct tgaagcctga atatattgaa gaaattatta ttggcatgac
     3601 gcaagcttgc ctcgaaaata atatttccct ggtcggtggt gagacagctg aaatgcctga
     3661 tacctatctg aagggagagc atgatttggt aggtattatc actggtgtag tggaaaagga
     3721 tgaagccata ctaggaaaag atataactcc aggcgattca gtcttggcat ttccttccag
     3781 tggcttacat accaatggat actcactcgc cagaaaactt ttgtttgaac taggtggata
     3841 tactgtagaa tcccggcttg acgagttgga tgctgcagtt ggtgaggctt tactggtacc
     3901 tcatatcaat tatacaaatc ctgttctaca cctgttgaat aataaaatcc ctatcaaggg
     3961 aatggctcat attactggcg gaggtgtact ggaaaacatt ccgcgagtat tacctaatta
     4021 ttgttccgtt gaaatcaata agaattcatg tccggtgttg ccgatcttta atgtgttaaa
     4081 agcattgggg aaactggatg atcaggaaat gtatcgtact tttaatatgg gaataggatt
     4141 ggtaatgata gcttcacctg gtgttgtttc tgaaattcat acagcgctaa aagaattccc
     4201 taatttccct gtttatgaag taggacaagt cgtatcagga aaaccagaag tgagattatt
     4261 gtgatgaaga ttgcagttgt tcagtttcca ggctccaatt gcgaacgtga gacaatgctt
     4321 gcggtgcaac gagcaggtat gactccagtt gagtttctgt ggaatgattc atcacaaaaa
     4381 ctgcgggaaa tggatggtta tattattatt ggtggttttt catatgagga tagatcaaga
     4441 gcaggaatta ttgcagccct tgatccggta atgaaagaaa taaaattgca gagtgaattg
     4501 ggaaaacctg ttcttggtat ttgcaatgga gcacagatcc ttgttgaaac aggcctcgtt
     4561 cctggtttaa aaaactatca aattggtatg gctctgactg agaataagcg aattcaggat
     4621 ggaaaaattt taggaacagg attttataac agctggattc atatgcgaac tggtgaccga
     4681 gtgaggcaaa atgcattcac ccgctattta tcaaaggatt ctgtattatc aattcctgct
     4741 gcccatgctg aagggcgttt tgtgatgcct tttaaattat tgcaagaaat tgaagaagaa
     4801 ggtttaaatg cgtttcaata ttgtgatgcg cagggaaaca gagatgataa tttccccgtt
     4861 aatcctaatg gttctctacg gaatattgcc gctattatta ataaagcagg taatgtcatg
     4921 gccataatgc ctcaccctga acgtacattt gcaggagatc ctatatttca ttcaatgaag
     4981 gaatatatta aggagaaaaa aacattcaag cagcaaacat tatcttatca accagagcca
     5041 gttaaaataa ttccttatga caaaaaagac aatgcccatg agttgattgt caaattaatt
     5101 attaccgata atgctgcttt aacagtacaa aatacgctaa gacagttagg tataccagtc
     5161 acagtgaatc gtatgatgtg ttggcatgtc gatacaaatt ttcctgaagc aatcgaaaaa
     5221 ataaagcaga caggggtttt atataatgat cggaaagagt atttggtaac gcctgagcaa
     5281 atattttcat cagcagccaa ggcttttttg gttaaatcaa aagacgatat gatcgggcaa
     5341 cagaagttgc aaatgcttcg ggatcatttc tcaattgatg gcgttgatgc catacatcat
     5401 ggtgttttat ggatatttag cagtgaaaat gttaaaatac aagaattggc agaatccatt
     5461 ttagctacta atattatctt taaccctaat gctcatatct gctatgaata ccaatgtccc
     5521 tagtcaagag gatcttcttg ctgcgttgcc tttttgctta accgaaacct ctttaccttt
     5581 cggcaaaaag tataagggga aagtaaggga tacctatgat cttggcgatc agttgatttt
     5641 ggtaactaca gatagacaaa gtgcctttga tcgatgtctt gctgctgtac cttataaagg
     5701 acaagtattg aatttaactt cagtatggtg gtttaaaaac actcaatcga ttgtacctaa
     5761 ccatttaatt gccgtacctg atcccaatgt ggcgatagcc aaaaaatgca aaatattccc
     5821 tgttgaattt gttgttcgtg ggtatatttc ggggagtaca agtacgtctt tatggacgca
     5881 ataccaaaaa ggggttcgtg agtactgtgg tataacattt cctgatgggt taagaaaaaa
     5941 tcaaaaactt gagagcccag tgataacccc tactaccaaa gaaacgatcc atgacaggcc
     6001 gattagccca cacgaaatcg tcgcagaagg ctggatgacc caagaagact gggatgaaac
     6061 cagcagttat gctttaaggt tatttcaaca tggaatggaa gtagctcagc aacatggttt
     6121 gattttggtg gataccaaat atgaatttgg ccgtgatgcg gaaggaagaa ttgtattagt
     6181 ggatgaaatt catactccag attccagtcg ttattggtta tttaacagct atcaagagcg
     6241 atttgatgcg ggtaaggagc cggagaatat agataaagaa tttttgcgct tgtggtttgt
     6301 tgatcactgc gatccttata aggatgaagt attacctcaa gcaccacagg agttaattgt
     6361 tactttagca tcaagatata tccaattata tgaaatgatt acaggtgaat cgtttgtgta
     6421 tgacagtaat cctgggccag taaatgatag gattttgcat aacatacaac gttggttagg
     6481 ttaattttta gccgggggaa aggcttatgt gtggcattgt aggaatttac agtcatgaac
     6541 ccgttgctgc tgagctttat gatagcttga ttcatttaca gcaccggggg caagatgcct
     6601 caggtatttt aaccggagct gatcgttttt acttcaaaca tggacttgga ctggtcagag
     6661 agtctatttt ctctgaagat attcttaaac tgaaggggaa tatcggcatt gctcatgtgc
     6721 gttatcccac ggccggaggt tatagtgaag cggatgttca gcctctttgg atagggagcc
     6781 ctcgcgggat agctctggct cataatggaa atttagtgaa ttatggagag ttggttgatg
     6841 atttatgcgg aaaacagcat aggcatttaa actctactct tgattcagag gcacttttat
     6901 tacttctggc agactattta gccaagggct cttattccga tcatgatgaa gagcaattct
     6961 ttcaattatt atgtatggca gttaatgaaa tacatcaacg agttcaaggt tcctattcgg
     7021 ttgtgagttt agtaattggc aaaggtttgg ttgcatttag agatccgcat ggtattcgtc
     7081 ctttagtttg gggtgagcga gtccatgaag acggtagtaa agattatatt tttgcttcag
     7141 aaaccacgcc tttttatgct ttaggttttg agcccaaggg tgatttgctg ccaggtgaag
     7201 ttgcttacgt aaatcagcaa ggacaattgt acagaaaagt gctttcatca aaaaaattta
     7261 ccccttgtat atttgaatac gtttacttcg ccagaccaga tgccacatta gacgatgtga
     7321 gcgtttatcg agccagatta cgtatggggc aaaatttagc taaaagctgg aaaaagagat
     7381 ttcctgatac actacctgat gtggtgattc cagccccttc aacagcaaat actgccgcat
     7441 tagcttttgc aaatgagttg ggaatacgtt attccgaagg tttatataaa aatcctttta
     7501 ttggacgtac ttttatcatg cctaatcagg agtcgcgatc aagacaggtt cgctataaat
     7561 taactcctca gaaaacggaa attaataaaa aaaaggtatt aatcgttgat gatagtatcg
     7621 taaggggaac tacctcacgc gaaatagtca gaatgatacg agaatatgga gcatcagaaa
     7681 tatattttgt atcaacctgt ccaccaatta aaaacccttg tttctacggg attgatatcc
     7741 catcgcgtaa aaaattgatt gcagcccata aaacggaaga ggaaatccgt caatttctag
     7801 gggtggataa attattatac caaactcagg aaagccttat agaggctgtg tctcgcaggg
     7861 ggcaatatga catgagcaag ccttgtatgg cttgtatgaa tggcaaatac atttgtggac
     7921 agattactga agaaaaaatt aaattgttgg agaaaaaaag attaaccgag aatgaccaag
     7981 aaacagagat aggagagatg tcttgatcaa tatccttatt gttggttcag gtgcgcgtga
     8041 gcatgcaata gctacagcat tgagtaaatc agagaataaa ccaaagattt attgctttgg
     8101 aacaactcgt aatcctggta tccaaagact cacctcagat tattgggtag gtgatattac
     8161 agaccctgag caagttaacc agcgagcaaa aaaatgggaa attaatttgg ccattattgg
     8221 tccagaagcg cctttggaac atgctgttgc tgatgctttg tgggattgtg gaattcctgt
     8281 tataggtccc aggaaaaatt tggctaaaat tgaaaccagt aaagcgttcg ccagagagtt
     8341 gatggaaaaa tacggtattc caggtttgcc taagtataaa gtatttacca gtttagaagg
     8401 aataggagag tttttatcag agttaggtga aaataatttc gttgttaagg ccaatggttt
     8461 aatggcgggc aagggtgtaa aagtaggtgg ggaacatctg ttgtcatttg aagaggcgtt
     8521 gaactattgt aaagaaattt ttactcataa tcagtcttta gtcattgaag aaaaattaat
     8581 aggtcaagaa ttttcattta tgtgtttttc tgatggcatc tcattagttc caatgcctat
     8641 tgtgcaagat cataaacgag cttatgagca tgatgagggc cctaatacag gtggtatggg
     8701 gagttattca gcggcagatc acagcttgcc atttttgtct ccagaggatg ttcgtgctgc
     8761 ttttgcaatt aatcaatcag taatcaatgc gcttatgacg gaaatgtctg ataaatacat
     8821 aggaatactt tatggcggat ttatggccac cgcacatggg gtgtatgtga ttgagtttaa
     8881 tgctcgcttt ggagatcctg aggctcttaa tgtacttagc ttgttagaat ctgattttgt
     8941 taaattatgt attgcgatgg ctaacggcaa tttacaaaaa gaatccgttc gctttttgaa
     9001 tcaagccaca gtttgtaaat acgctgtccc tgatggttat cccgataatc caatgcgcca
     9061 tgttgaaatt gatatttctg ctgttgaacc taaagatact ctatatttgg ctgcagtgaa
     9121 ttttaaggat ggcaaactct atgccacagg ttcgaggaca gctgcttatg tcggtatagc
     9181 gaatactatc agtgaggcag aagaaattgc tgaacaacag ataaaaaata taaaaggccc
     9241 tttatttcac agaaaagata ttggcacatc gcctttaatt aatacacgcg ttgaagtaat
     9301 gcgtaggttg cgtgccttat gattcgttta ggaatattag gatctactcg tggaaccaat
     9361 atgttggctc ttgttgatgc aatcaatgag ggaactctta aggcaaagat agagcttgtt
     9421 attagtaata aacctgatgc cattattctg gaaagagcca aatcgttggg tttgaatgca
     9481 cagtttgtca atcctgaggg attaaacaga attgattttg ataaaaaagt aagtgacata
     9541 ctgatcaacc atcagattga tttgattgtt ttgatcgggt acatgagaat tttgtcggcg
     9601 gatttcgtaa ataaatggaa taatcaggta attaatgtac atccttcttt attgccagca
     9661 tttgcaggga aaatggatat agatgtacat caagccgttc ttgattccgg cctaaaggaa
     9721 acaggttgta ctatccattt tgtcactgaa gaggtagatg ctggtcctgt tattttgcaa
     9781 aaaaaatgcc ctgtcttaga aggtgataca gctcaaacgt taaaagcaag ggtgcagcag
     9841 ttggaaggaa tggctttggt agctgcaatt aacctgattg catcgaaagg tctctaatac
     9901 tagattgtat ttccagtcag agcagtatgg tcttcatttc tatgagagta gtttttttct
     9961 ttttcaattg taaaaaaagt gtttaacgaa gtttctttaa actcaaataa atggattcca
    10021 ttcagtttaa tactggtgtc tgtatgaact cgattatttg cagcggattt taaaacatgt
    10081 gaatataatt tttcacgtaa gctatcgcaa acacttgggt tatttttaga aaaatattga
    10141 ttgcaatagc ttttggcaat taattgaaaa tcttctttag ttaatgtttg tcgtttttcc
    10201 tgttcaaggt taacaacaac aatatcaatt ggagcactta attcattacc attttttctt
    10261 acgacaataa tgagatgcgg ttttccagta agtcgaacca actcactgga gaatccgggt
    10321 agtgatgcag gtagtgtttt taataatgta tttaattgtt tgataaatag aaaattattc
    10381 tgtaatccaa gccatccatt ttcgtgggtt cttggattct ctgaaaaatc ttgagatgtg
    10441 aaacctatat ttaaactttc actggcaagt agcctggaaa atttttcttg tacatgatct
    10501 atgccggcat ttatttggta tttaatatta tcaatatgag atactaaaag acgtttttgc
    10561 atttcaggaa aacttgataa acttcggtaa agttcctgca tccacttatt ttccgagttt
    10621 tctatgaaga aattctcttc tgccattttt tctaaaattt tgtgagcaat aactggtttt
    10681 tcactaaaaa tacgatgttc acgttgtggg taataagatt tatcaacggc gccttttctc
    10741 tgaaatactg tttttgcggt gatatcgcat gtttcatcac cagataataa aaaccatccg
    10801 ccactggctg taagagcgga taaagccagt ttttctaata ccagagtttt aaattcttgt
    10861 gctgtttcat cttgattatc ttccaactcc tttttaattt gatcaacata agagtaaagt
    10921 aaggttgttt tcaaaggctc tttaagttca tgtggttttt cttgctcggc gttaaactta
    10981 aagtacaagt cttgatagga agacattaag tcattataaa aagcgtcaga tttcagtctt
    11041 tttttggcca tttgaaataa aatagtcatc gccaatgaac gatagtcatg attcccacaa
    11101 aaattaatgc agcctccctt cgtattttca cgccatgtcc catctttttg ttggaagact
    11161 acactcgcct ttaattgctc ttccaatgtt atttcataag gcagatcagc gctggcaaga
    11221 tcaaattcaa cattataagc ttctgtagtg atgtgagttg ggcctatacc aggtaatttc
    11281 aaataattta cttcatccgc aagtggtcgt ttactaaata aagcttcatc aaggataacc
    11341 ggcttgggtt taccattttc agcagcaaga gagtgaatat aggcatatat ttcacttctt
    11401 actgcaggat gtacataacc tacagcatcg actcgaacac catcaaaacc gtattcgatc
    11461 atgtaacgtt tgatataagg ttgccaaaat tcggttatta tttcttttct tatgctttca
    11521 tcatcgtaat taaagccgcg aacatctttg aagtcttcat gaactccttt gaaccaatga
    11581 ggcttatcct gacacaaggg agaatcaatg gcgacatgat ttaaaaccag atcaaacatt
    11641 ggaactattc cgaggttcct tgcattttga gtgaataact gtaaagcttc tgagtttaat
    11701 cgttgagttg tctccatggg gtcttcagga gagcccaaac tgaattgagg attaaataat
    11761 aacgggtgac tcatggcata taagcttcta gtgacctcat ttcctgtttt tacaccatta
    11821 tttttatccg ttttaaaaaa accgctaatg tcaccgggca tttgcatggg attaatccat
    11881 aaggcattaa accccatctg ctttaagaca ggcaaataat caatcatatc attaatagaa
    11941 tggaactgag ttgggtacat gtcatagcat gcaataatgc catttccttc cattaaaaaa
    12001 tgatatccca tagaatctgt caccataaaa aattattaca ctacaaaact gagaaaaatc
    12061 gaattgttgg ttacaactcc acttttatca gtcgaatcgc ttgttattat atcgttaaaa
    12121 agacaaatta ggagctgatt cattgtccaa ttgttcttct tctggtttat cttttacttc
    12181 atgtgtgaat tcatccagta tctgtttggc tttttcagat gctttatcat aaattttatt
    12241 aattagagca gatggtattt tgcctttggc aatagtttct gatattgctt tatcccaaga
    12301 ctccatctgc gttttattgc catgaatcag gcccttgcca aagtaggcat tattgatagc
    12361 acttatagaa tattggtgct cagataattt tcgggcaatc aagagcgctt ctaatgcctc
    12421 ctggtaagat aaagaagggg cgttcgataa aatggctaaa tttaaacaag tcttataaaa
    12481 taataaatat cctggagtcc aatgatattg actggccttt tgagcatagt gcaatgttaa
    12541 caaagaataa tcattcaaac ccattttgta tttttcataa gcccaggttg ataaagagtt
    12601 ataagcataa aaacagtgtt gatgagcgct cttcttaaga aactctaatt cattatcacc
    12661 aaatggttta ccttcttctt cccggcattc agctgcttgc ccataaaaat agatcccttt
    12721 tactaattgg gacactgtga gtggtgggta ttcttcaaat gaaaaatgtt cttcgttaaa
    12781 attttgcaga tgagtccgcc aaatatcagc ttgctctgct tcttcacaaa aatctgtaat
    12841 tgttttactt aattcggtat attttatgag ggtagtaaga tcaagattat ttaagagcat
    12901 tttcgctctg gcaaaattgt tttcttttat cagattttcg atgatgttta ttttctttaa
    12961 ttcgctatat gacgatttca gccaatcatt ataggagcta cccataaatc ctccacaaaa
    13021 attttaagtt caagacatgt tagagaataa agtctaagtc ttgacaccaa tatttgttaa
    13081 taattggtga tataagtgcg gtaatatttt aacacaattt gatttattta aaaactgtca
    13141 atatcaattt agccgctcaa taacattcaa agatgagaaa agtacaagat aaatttccct
    13201 attggttata caaaagctat gtttgtttgc tttttaagcg caagaggcat a
//
LOCUS       DBNCWK010000085        13165 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.085, whole genome shotgun sequence.
ACCESSION   DBNCWK010000085 DBNCWK010000000
VERSION     DBNCWK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13165
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.085"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..2127
                     /locus_tag="AC06IO_002025"
     CDS             <1..2127
                     /locus_tag="AC06IO_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HIO9875201.1"
                     /translation="HIDLGELAKNDSLLACLVLLTRYYQNPFSPPSLIARLPLVNSKL
                     SVDMFSRAAARASLDSEVKTIKLEQITDSDIPLVLIKKNGEAVLLILEDDGKKAILDP
                     KQPDSRIDLNQIADELSGEVIFVEQQYKPSTRAHETLSKEQKNWFWKVVIKSWPLYSE
                     VLVASLLVNIFALVVPLFSMNVYDRVVPNHAIETMWVLASGVALVFLFDMLLKSLRSY
                     FIDVANKHSDKELSALIFQQVLGLSMANRPKSVGSLANTVQSFEVFRDFITSTTMTVL
                     VDLPFVFIFLFVIYLIGGVLFWIPFLVLPTIFIIGLLLQWPLIRLTRKSYQYGAEKQA
                     TLFESLATIETVKTTGAESVLQSRWERIINESSKNSMQLRFVANTSISITNLAQQIAT
                     IAIIIAGVYMISEGELTTGGLIACTILTGRALAPMAQVSTLFTRYFQSVNALDSLNKI
                     MQLETDTTEETRYLHRPSLKGAVEFKQVSFHYKEDETKVLQNLNFKINAGERVAIIGR
                     VGSGKSTLGKLILKLYVPNEGTILLDGTDYKQINPDDLRQQIGYVPQDIVLFYGSIRD
                     NICVGAPFINDPALLEAAEIAGVTSFTNNHPKGFDRQVGEKGAELSGGQRQSVALARA
                     LIMSPNILLLDEPTSAMDDNNEKQIKKNLNEYLSDNRTLILITHKISMLELVNRIIVL
                     ENGKIIADGNKEAVLSALRTGVTVRK"
     gene            2131..3267
                     /locus_tag="AC06IO_002026"
     CDS             2131..3267
                     /locus_tag="AC06IO_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HIO9875202.1"
                     /translation="MKKTHSSKTFTHIILWTSLLFFIVAIIWANYAILDEVTTGQGKV
                     IPSSEVQVIQNLEGGIIQNIFVKEGQIVKKDQILMQIDNTRFMASYAEAEKKIDALEI
                     EIIRLNAEISDTKPVFPDKFIKTYPHLVQDQLSLYESRMRELNQLKKSLELAQKELDL
                     TRPLLKGGSVSEVEVIRLERSVSEIKGNIEKFKSEELDKLNKARTELFALVEANKADK
                     DRLTRTTVRSPVYGIVKQIKTTTIGGVVQPGSDLLEIVPLDDTLLIEAKIRPSDIGFI
                     HPGQKAMVKITAYDFSIYGGLEGVVEHISADTIIDEKTDKKEESYYIVKVRTEKNYLG
                     TEKKPLPIIPGMQATVDILTGQKSVLQYLLKPIIKAKQSALRER"
     gene            3358..5880
                     /locus_tag="AC06IO_002027"
     CDS             3358..5880
                     /locus_tag="AC06IO_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIO9875203.1"
                     /translation="MPSMVLPKPSNAYTFAYYLTITFAMLTLILGLSVLIGWHFGIEF
                     LIQFQSGSVAIVYNTALCFIVLSLAILFSLNCYYKSSLILSIIVFVFSGLGFSQHILG
                     VNLGVDEIFFHHYHAFKNAYPGRMAANTAFCFLMISAALIMMNLKCYRSFNNSLAGAL
                     GFLVFCLAILFISGYFSEIQHAYQWGDKTPMSINTAIDFFMLSSATIGLYWRNSIYFQ
                     LNSSIAMPYLCSFCIILTFTLLSFEILEKENRLNLSFSVATFVIGIFLSFLFGLVIRL
                     WQLAKLSAIAAKYAFLEVEATLESTADGILVVDKESKVINYNQRFLDMWRQGINPPRI
                     INYLDIKEEINKQLVNKREVIHRINQTIKHPDHKNSFELKLKGNLYYDCYTQPLKLDE
                     DIIGRVWSFRDITIPKRLEMELSHQSTHDTLTNLPNKVLAIDMLGYAMKGAISDKKIV
                     GVYLLDLDRFTQINDVFGHSKGDEIIKIIAKRLKDCVPVEHILGRISGDQFIVIASLD
                     EIREAVLGVTRLLAAFHEPVKMNSHCIKMSCSIGVSFYPRDGNTVDVLLSKADIALSR
                     TKIEGRNNFQFYTRDMNTYTLEHILLENELRDAIKKREFVLYYQPILELRSLNVNGFE
                     ALIRWNHPRNGLLLPEKFIQLAEEIGIISEIGDWVLTTVCKQIKTWQKQGVNNLKVAV
                     NISPKHLRFRHLPIRINEILSEFQVKSESIELELTESILIDKSSGIIDTLNELKEIGI
                     KISIDDFGTGYSCLSYLKELPIDIIKIDKEFIKDLITNQPNQALVKAIIAMAKNLGLH
                     LIAEGIENKHQLEILQNLGCHYGQGYYFYHPMPPEKCTQFLQ"
     gene            complement(5882..6451)
                     /locus_tag="AC06IO_002028"
     CDS             complement(5882..6451)
                     /locus_tag="AC06IO_002028"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HIO9875204.1"
                     /translation="MTIPDKIKAVYEKSTCLYTSNEVEAALDRMAIKIHETLQDKNPV
                     IICVMVGGLVPLGNLLHRLDFPLEVDYVHATRYRGDLTGGDILWKVRPSSNLAGRTVL
                     VVDDILDGGITLAAIVNEIKAMGAAEVYSAVLVDKYRKRVPNGLQKADFVGLQVEDHY
                     IFGYGMDYHEYLRNAPGIFIVHPDHEASK"
     gene            complement(6494..6820)
                     /locus_tag="AC06IO_002029"
     CDS             complement(6494..6820)
                     /locus_tag="AC06IO_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HIO9875205.1"
                     /translation="MTKKSLFKISFANQDEIYEIYARSIKESDMFGFLEVEELVFGEQ
                     TALVVDPSEEKLKMEFCDVKRIFIPVHSIFRIDEVSKQGTSKVKDNLSHGNKVRPFPG
                     AGKIKD"
     gene            complement(7003..7746)
                     /locus_tag="AC06IO_002030"
     CDS             complement(7003..7746)
                     /locus_tag="AC06IO_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIO9875206.1"
                     /translation="MLIEHQIKHYRTLYEWFQSPLGVFVAKEFTDQLEPVKKFFKGET
                     LLQLGDCATNPWLNLLDFKSKWIASPFSSTNRIHLECSLNQLPLNRDTLDCVIVPLTL
                     EPFGNNFSLIDEIDRILKPMGFLIFLCINPWSLWGGAMKWGVLNCYGNRTVKLRSAFN
                     LNRIFLQRGYKQCSLTNFCYIPPVNSQSLIKKLTFFDEIGKMIWPFPSGFYCYITQKY
                     EYISPSLTIEPSIESVKKEYDSTLQPATN"
     gene            8005..9729
                     /gene="pilB"
                     /locus_tag="AC06IO_002031"
     CDS             8005..9729
                     /gene="pilB"
                     /locus_tag="AC06IO_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HIO9875207.1"
                     /translation="MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKMSLLQ
                     YIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTIPANLVNEKLIKRHAMVPLFSRG
                     TNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
                     DSGDLEGLEISADDEDQDSDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYERE
                     YRIRYRQDGILHEVATPPASLSSRITARIKVMSNLDISERRIPQDGGFKMKISKSRAI
                     DFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGP
                     TGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFL
                     RQDPDIIMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIA
                     SSVTLIIAQRLARKLCNQCKAVRDDFTNQGLIELGFKESDLVNLKLYKAVGCEQCTSG
                     YRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKEGITTIE
                     EVNRVTVD"
     gene            9735..10955
                     /locus_tag="AC06IO_002032"
     CDS             9735..10955
                     /locus_tag="AC06IO_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIO9875208.1"
                     /translation="MDKNSPTLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGI
                     VTNKVIKKRKPLFDRKNKKITQADITVFSRQLATMIESGIPLVQAFDIVAKGQSNKRL
                     KDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
                     IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITM
                     SEFMQAYWYIIFGALGGVVYSFFYAKNHSLEFAQTIDRVMLKFPVIGPILEKAAIARF
                     ARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATGQQMFTAMENTHLFP
                     NMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLV
                     VAMYLPIFKLGSAV"
     gene            11001..11864
                     /locus_tag="AC06IO_002033"
     CDS             11001..11864
                     /locus_tag="AC06IO_002033"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIO9875209.1"
                     /translation="MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWK
                     EQCCELFHFEQRKEKIKLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCDSP
                     ISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
                     GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAA
                     FGAWLGWMYLPLILLISSITGAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGD
                     SIINWYIGYWM"
     gene            complement(11878..13083)
                     /locus_tag="AC06IO_002034"
     CDS             complement(11878..13083)
                     /locus_tag="AC06IO_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIO9875210.1"
                     /translation="MNQNDASKKDNSNSNQLDTELIGSYQSITIGFSKDELASFSKIG
                     QHKHYTAGSIVYNENDLADSFFIIIDGVAEIFKKTIDEFDEHNIHILATLSKDDVVGE
                     MALIENSRRSASVKAKTDLSVIEYPLEVVKAKPKLNLMLIKNMAQILSRRLRFTNEVT
                     VKNMQQALKEASGRNTLGVFIIALLWIICLYTLSLHFLVTVIHQVTVTTVLSICLISI
                     FAVAILIAMHFTGLPYSRFGITAKDWGKTILEALAFTIPIMLFVLIIKVFFIIGIDHL
                     DNTPIFSGTKRFLVNNEVDWEYYFAMIFLYSLFAPIQELMARSALQSTFFHFLPGEKL
                     FRQWNGIFLSNLIFATMHTHLGLTFASLTFIAGLFWGWLFHRQKSLLGVSVSHVILGV
                     WSLFIVGLR"
BASE COUNT         4238 a         2196 c         2502 g         4229 t
ORIGIN      
        1 catattgatt tgggtgaact agccaagaat gactctctgc tagcctgcct ggtattatta
       61 actcgatatt atcaaaatcc gttttcgcct ccatcactta tagctcgttt gccattggtc
      121 aatagtaaat tgagtgttga tatgttttcg cgtgccgctg ccagagcctc tttggattct
      181 gaagtcaaaa ctataaaatt agaacaaatt actgattcag atattccctt agtattgatt
      241 aaaaaaaatg gcgaggccgt gcttctaata ctggaggatg atggtaaaaa agcaatcctt
      301 gatcccaagc aacctgattc ccggattgat ttgaatcaaa ttgctgatga gttaagtggt
      361 gaagttattt ttgttgagca acaatacaaa cctagcacca gagcacatga aactctttcc
      421 aaagagcaaa aaaactggtt ttggaaagtt gttattaaat cttggccatt gtattccgaa
      481 gttcttgtgg cctccttgct cgtgaatata tttgcactag ttgttccatt atttagcatg
      541 aatgtgtatg accgtgtggt tccaaaccat gctattgaaa ccatgtgggt gcttgctagt
      601 ggtgtggctt tggtatttct ctttgatatg ctgctcaaga gtttacgatc ctatttcatt
      661 gatgtggcaa ataaacacag cgataaagag ttgtctgctt taatatttca gcaagttctc
      721 ggattaagta tggctaacag gccaaaatcg gttggttctt tagccaatac agtacagtca
      781 tttgaagtat tcagagattt tattacttcg actactatga cagttcttgt tgatcttcct
      841 tttgtattta tttttctttt tgttatttat ttgattggcg gggtgttgtt ttggatacct
      901 tttttagtgc tccctactat ttttataata ggtttgttgc tgcaatggcc tttaattcga
      961 cttacaagaa aatcctatca atatggtgct gaaaaacaag cgacgttgtt tgagtcttta
     1021 gctactatag aaactgtaaa gacaactgga gcagaatcag tcctgcaatc acggtgggaa
     1081 agaataatta atgaatcttc taaaaactct atgcaattac gttttgtagc caatacaagt
     1141 atcagtatca ctaatctggc ccaacaaatt gcgactatcg ctattatcat tgcaggtgtt
     1201 tatatgatct ctgaaggtga gttaaccaca ggtggattaa ttgcctgtac catattgact
     1261 ggaagagcat tagcccctat ggctcaagta tccactctat ttacacgtta cttccaatca
     1321 gttaatgctc tggactcatt aaacaagatt atgcaacttg agacagatac cacagaagaa
     1381 actcgttatc tccacagacc ttctctgaaa ggtgccgttg aatttaaaca ggtgtctttt
     1441 cattacaagg aagatgagac gaaggtatta caaaatctta attttaaaat taatgctggc
     1501 gagcgcgttg caattatagg acgagttggt tcaggtaaat cgactttggg aaaactaata
     1561 ttaaagcttt acgttcctaa cgaaggaact attttgctgg atggcaccga ttataagcaa
     1621 attaatcctg acgatttgcg tcagcaaata ggttatgttc ctcaggatat tgttttattt
     1681 tatggaagca taagagataa catttgtgta ggtgctccct ttataaacga ccctgctctt
     1741 ttagaagccg ctgaaatagc tggggtaacg agttttacca ataatcatcc caaaggcttt
     1801 gatagacaag taggagaaaa aggagctgaa ttatcaggag gacaaagaca atctgtcgct
     1861 cttgcccgtg ctttgattat gagccctaat atcttgttat tggatgagcc tacttcggca
     1921 atggatgata ataacgaaaa gcaaatcaag aaaaatctta atgaatactt atccgataat
     1981 cgtactttga ttttaataac tcataagatt tccatgctgg agctggtcaa tcgtattatc
     2041 gttttggaaa atggcaaaat cattgctgat ggtaataaag aagctgttct aagcgccctc
     2101 aggactgggg taacggtgag gaaatagtct atgaaaaaaa ctcacagctc aaaaaccttt
     2161 acccatatta tcttatggac ttccttgctt ttttttattg tagcaattat ttgggctaat
     2221 tatgccattc ttgatgaagt aactacagga caaggaaaag taattccctc tagtgaggtt
     2281 caagtcatac agaatcttga aggcgggatt attcagaata tttttgtcaa agaaggacaa
     2341 atagttaaaa aagatcaaat tttaatgcaa atcgataata ctcggtttat ggccagctat
     2401 gccgaagcag agaaaaaaat tgatgcattg gaaatagaaa ttataaggct taatgctgaa
     2461 attagtgata caaaacctgt gtttccagat aaatttatca aaacctatcc tcatcttgtg
     2521 caggatcagt tgtctctgta tgaatcacgg atgagagagt tgaatcaact gaaaaaatct
     2581 ctagagttgg cgcaaaaaga attggattta actcgtccat tattaaaggg tggatccgtt
     2641 tcagaagtag aggttattcg tctggaaaga tcagtaagtg aaattaaagg aaatattgag
     2701 aagttcaaat cagaagaact ggataaattg aataaagcca gaacagagtt gtttgcgctg
     2761 gttgaggcaa ataaagcgga taaagatcgt ttaacaagga ccacagttcg ttctccagtt
     2821 tatggtattg taaaacaaat taagacgaca acgattggtg gtgtagttca accaggtagt
     2881 gatttacttg agatagtccc gcttgatgat actttattaa ttgaagcaaa aatacgccct
     2941 tctgatattg gttttataca cccaggccaa aaagcgatgg ttaaaattac tgcttatgac
     3001 ttttctatct atggtggatt ggagggtgtt gtagagcata ttagtgccga tactattatt
     3061 gatgagaaaa cagataaaaa agaagaaagc tactatatag tgaaagtgcg aacggagaaa
     3121 aactatttgg gaactgaaaa aaaaccattg cctatcattc caggtatgca agctactgtt
     3181 gatatactta caggtcaaaa atcagtcctt caatacctct tgaaacctat aattaaagct
     3241 aagcaatccg cacttagaga acgttgatga agttgttacg gtattatgaa aaaatggtct
     3301 attattataa tattagtctt atttttgcaa ggatatcatt gctagggata taacattatg
     3361 ccatcaatgg tactccctaa accatctaat gcttacactt tcgcatatta tttaaccatc
     3421 acctttgcca tgctaacact aattttgggg ttgtctgtct taattggttg gcattttggt
     3481 attgagtttt taattcaatt ccaatcaggt tcagtagcaa ttgtttataa tactgctcta
     3541 tgctttattg ttttgtccct tgctattcta ttttctctaa attgctatta taagtcgtct
     3601 ttaattttaa gtattattgt ttttgttttt tctggtttgg gtttttccca gcatatttta
     3661 ggtgttaacc ttggggttga tgaaattttt ttccatcact atcatgcctt taaaaatgct
     3721 tatccaggcc ggatggcagc aaacaccgct ttttgctttt taatgatatc ggctgctctt
     3781 attatgatga atttaaaatg ctataggtca tttaataatt cattagctgg tgcacttggg
     3841 tttctagtat tttgtttggc aattttattc ataagtggat atttctcaga aattcaacac
     3901 gcttaccaat ggggagataa aacccccatg tcaattaata cggcaattga tttttttatg
     3961 ttgtcctcgg caacaatcgg actatattgg aggaacagta tctattttca attgaattcc
     4021 agtattgcga tgccttatct atgctcgttt tgtattatat taacttttac attactatca
     4081 ttcgaaattt tagaaaagga aaataggtta aacttaagtt tttcagtcgc aacatttgtt
     4141 attggcattt ttctttcttt cctttttgga ttggttatca gattgtggca actggcaaaa
     4201 ctatcagcta tagcggctaa atatgcattt ttagaagtcg aggctaccct ggaatcaaca
     4261 gctgatggta ttttggtagt ggataaagaa agcaaagtga ttaattacaa tcaacgtttt
     4321 ttagacatgt ggcgccaggg aataaatccg ccacgtataa taaactatct agatatcaaa
     4381 gaagagatta ataaacaatt agtgaataaa agagaagtaa tccacagaat taatcaaact
     4441 attaaacatc ctgatcataa aaattcattc gaattaaaac ttaaaggcaa tctttattat
     4501 gattgctaca cccaaccact aaaattggat gaagatatta ttggtcgcgt ttggagtttt
     4561 agagatatta ctattcctaa aaggcttgaa atggaattat cccatcaatc tacgcatgat
     4621 acactcacca atttgcctaa taaagtgttg gctatcgaca tgctgggata tgcaatgaaa
     4681 ggtgcaatta gcgataaaaa aattgttggg gtgtatttac tggatttaga tcgatttacg
     4741 caaattaatg atgtatttgg tcatagcaag ggagatgaaa taattaaaat tattgccaaa
     4801 cgattaaaag attgtgtgcc agttgagcat atccttgggc gaattagtgg cgatcaattt
     4861 attgtaattg cgagtcttga tgaaattaga gaagctgttt taggggttac acggttatta
     4921 gctgcctttc atgagcctgt aaaaatgaat agccattgta tcaaaatgag ttgcagcata
     4981 ggtgtttcct tctatccaag agatggcaat acagttgacg tgctgctatc caaagctgat
     5041 attgctttgt ctcgaacaaa aatcgaaggc cgtaataact ttcaatttta taccagagac
     5101 atgaatacct atactttaga acatattcta ttagaaaatg aattaaggga tgctattaaa
     5161 aaaagagaat ttgttttgta ttatcaacct attctggagt taagatcttt gaatgtaaat
     5221 ggctttgaag cgttaatccg ctggaatcat cccagaaatg gattgcttct ccctgaaaaa
     5281 tttattcagc tggcggaaga aataggaata attagtgaga ttggtgattg ggtacttaca
     5341 actgtttgta agcagataaa gacatggcaa aaacagggag ttaataattt aaaggttgct
     5401 gtcaatattt cgccaaaaca tttaaggttt cgacatcttc caatacgaat taacgaaata
     5461 ttatctgagt ttcaggtgaa atcagaatct attgaattag agttaacaga aagtattttg
     5521 atagacaagt cgagtggtat tattgatact ttaaatgagt taaaagaaat aggcattaaa
     5581 atatcaattg atgattttgg caccgggtat tcttgtctaa gttatttaaa agaattgcca
     5641 attgatataa taaaaattga taaagaattt atcaaagatc ttatcacgaa ccaaccgaac
     5701 caagccttgg taaaagcaat aattgcaatg gcgaaaaatc ttgggttgca tttgattgct
     5761 gagggaattg aaaacaaaca tcaacttgaa attttacaaa acttgggttg tcattatgga
     5821 caaggatact atttttatca tcccatgcca cctgaaaaat gtacgcaatt tttacaataa
     5881 tttatttact tgcctcatga tccggatgaa cgataaaaat ccctggtgca ttacgtaagt
     5941 actcgtgata atccatacca tagccaaaaa tataatgatc ctctacttgt aaaccgacaa
     6001 aatcagcctt ttgcaaacca ttggggacac gtttacgata tttatcaact aatacagcac
     6061 tataaacttc cgcagcaccc atggctttga tttcattaac tatggcagct agggttatac
     6121 caccatcaag aatgtcatct acgaccagga ctgttcgtcc tgcgagattg cttgatggcc
     6181 tcactttcca aagaatgtcc cctcccgtta aatcacctcg ataacgcgta gcatgaacat
     6241 aatccacctc tagaggaaaa tccaaacgat gcaatagatt acctaatgga actagacctc
     6301 ctaccatcac gcaaataatt acagggtttt tatcctgcag tgtttcatga atttttattg
     6361 ccattctgtc cagtgctgct tcaacttcgt tagatgtgta taaacaggtt gatttttcat
     6421 atacagcttt aattttatca ggaatggtca tttatttatt tccttagaag ttgtttatac
     6481 gggctaaacg aagttaatct tttatcttcc ctgctccagg gaaaggcctt actttattgc
     6541 catggctcaa gttatctttg acttttgatg tcccttgttt tgaaacttca tcaatgcgaa
     6601 atatggaatg tacaggaata aaaatgcgct taacatcaca aaattccatt ttaagttttt
     6661 cttcagacgg atcaactacc agtgccgttt gctctccaaa aaccagctcc tcaacttcca
     6721 gaaaaccaaa catatcgctt tcttttattg aacgagcata gatttcataa atttcgtcct
     6781 gattagcaaa actgattttg aatagcgatt tttttgtcat gtatctaacc ttatctaaaa
     6841 gcaggataaa gtatacaaaa agtaagggtg tatcttcaaa tatttaaagc agtttagttg
     6901 tctagagctt cttattagct ccaaatttat tcatttagac ttaatggtga caataattta
     6961 atctctttcg tatgtggaat attaatgaaa tataaatttc atctaatttg ttgctggctg
     7021 taaggtcgag tcatactctt tttttaccga ttctatggaa ggttcgattg tgagcgatgg
     7081 gctgatgtac tcatattttt gagtgatata acaataaaag ccagaaggaa aaggccatat
     7141 cattttgcca atttcatcaa aaaaagttaa ctttttaatt aaagattggc tatttactgg
     7201 tggtatatag caaaaattag ttaaggaaca ctgtttgtag cctctttgaa ggaaaattct
     7261 gttaaggttg aaagctgaac gcaattttac cgttcgatta ccataacaat taagtacccc
     7321 ccatttcata gcccctcccc ataaactcca agggttaatg cataagaata tgagaaatcc
     7381 cataggcttc aatattcggt caatttcatc aattaagcta aaattgtttc caaacggctc
     7441 tagtgttaaa ggtactataa cacaatccaa agtatctcta ttcagtggta actgatttag
     7501 cgagcactca agatggattc tatttgtaga agagaatggt gatgctatcc atttgctttt
     7561 aaaatcaaga aggtttaacc agggatttgt agcacaatct ccaagttgta gtaatgtctc
     7621 tcctttgaag aactttttta ctggttctaa ttgatccgta aattctttag caacaaatac
     7681 tcctaaaggt gattggaacc attcgtataa agtgcgataa tgttttattt gatgttcaat
     7741 cagcaaaata tgacctttaa tttattaccc tttattaact acaatacaac gagttttaga
     7801 tgcaaacaat ttaaatcttt caaatttttt attatttcca agagagttgc gttatagccc
     7861 gtgtttgcaa aaaattgata tgacaaagta accagaaata ggatttgggg gcaggtccaa
     7921 aacaccgggt tttgttcaaa aaaccatgca attataaaaa aatttaacta tatttaaaac
     7981 taagatacac tttgaataaa tgaaatggct ctagctacag aagaatatag attacaggga
     8041 atcggccagc ttctcgtgct ggaaaagctg ctagataaaa caaaagcaat tgaactacac
     8101 aagctggcag cagcggaaaa gatgtctttg ctgcaataca ttgtaaaaaa taaaatatta
     8161 tctgctgaac aaattgcact gacagctgct caaaattttg gcgtacccat gttggatatt
     8221 aactgcattg atgtaggcac cattcctgcc aatctggtta atgagaaatt aattaaacgt
     8281 catgccatgg ttcctctttt tagccgcggt accaatttat atctcgcaac agatgatcct
     8341 agtaaacaag cttcattaaa ggaaattcaa tttcacaccg gattaaatac tcatgcgata
     8401 gttgtagaaa cagataaact tagtgccctg attgataacc tgttaacagc aaaggaaagt
     8461 cagggcttat cagagtatgt tgaagactct ggagatctgg aaggtttaga aataagtgct
     8521 gatgatgaag atcaagatag tgatactgca acatcagtaa ctgatgatgc acctatcgta
     8581 aaattcgtca ataaaatttt attggatgcg ataaggcaag gagcttctga tatacacttt
     8641 gagccttatg aacgagaata ccgaatcaga tacagacaag atggtatttt acatgaagtc
     8701 gctacccctc ccgcaagctt gtcatctcgt attacagcac gtatcaaggt aatgtctaat
     8761 ttggatatat cagaacggcg tatcccccag gatggtggct tcaaaatgaa aatttccaag
     8821 tcacgagcaa ttgatttcag agtgagtaca tgtccaactt cggctgggga aaaagtggtt
     8881 atgcgggttc ttgattctgg tgctgctaaa ttaggtattg aagcattggg atttaatcct
     8941 gttcagcgaa caaatttcct gaaagcaatc cagcgcccac aagggatgat tttagtcact
     9001 ggacctactg gtagtggtaa aacagtgact ttatatacgg ctttaaatat attaaataca
     9061 attgaggtta atatttcaac agctgaagat ccagttgaaa tcaaagtgcc tggtatcaat
     9121 caggttaata ttaatcctaa agcgggccta accttttccg gtgcattacg ttcgtttcta
     9181 aggcaagacc ctgatattat aatggttggt gagatacgag accttgaaac agcagagatt
     9241 gctgtaaaag ctgctcagac agggcatttg gttctttcta ctctgcacac aaacagtgcc
     9301 gctgaaactt taaatcgttt agtaaacatg ggaataccaa cttttaacat tgcgagttcg
     9361 gttaccttaa ttattgcgca aaggctggca agaaaattat gcaatcaatg taaggcagtg
     9421 cgagatgact ttaccaatca aggcttgatt gaactaggtt tcaaggaatc tgatctggtt
     9481 aatcttaaat tatacaaagc agttggttgc gagcaatgta caagtggtta tcgcggacga
     9541 gtcgggttgt ttgaggtttt accgatgacg aaagagcttg gccaattgat tatgtcaggt
     9601 ggtaattcgc tggatatatt aaagttagct caatcagaag gtatgctgac tatttttcaa
     9661 tcgggtatag aaaaagttaa ggaaggaatc acgaccatag aggaggtcaa tcgggtaacc
     9721 gttgattaat ttggatggat aaaaatagcc caaccctatt aacctttcat tatcaaggca
     9781 ttaataaagc cggtcaaaaa atggagggtg atattcaagc cagaagcttg gcaatagcta
     9841 aagctgattt gcgtaagcag gggattgtta ccaataaagt aataaaaaaa agaaaacctt
     9901 tgtttgatag aaaaaataaa aaaatcacac aagcagatat tactgtattt agccgccaat
     9961 tagcgacgat gatcgagtca ggaataccat tagtgcaagc atttgatatt gttgccaaag
    10021 gacaaagcaa taaaagactt aaagacttaa tagaaacaat taagcacgat atcgaaacgg
    10081 ggctaacgct ggcagaatcc ttaattaaac atccactcta ttttaatgaa ttattttgta
    10141 atcttgtcga tgcaggtgaa aaatcaggct cattagatat catgcttgac aaggttgcaa
    10201 cgtataaaga aaaaattgaa atcataaaaa agaaaataaa aaaagctctc acttacccaa
    10261 ttgctgttat ggttgtcgca cttttggtta ctgcgggatt attaatctat gtggttcctc
    10321 aatttgaatc attatttaaa ggatttggag ctgatttgcc agcgatgact agagctgtga
    10381 tcactatgtc tgaatttatg caggcttatt ggtatattat ttttggagct ttgggcgggg
    10441 ttgtctatag ctttttttat gctaaaaatc attctttgga gtttgcgcaa accattgaca
    10501 gagtgatgct aaaattccct gtaattggtc ctattttaga gaaagcagca attgctagat
    10561 tcgcgaggac attatccatt acttttgctg ctggattacc cctggttgaa gcattgaaat
    10621 ctgtagctgg agcgaccggg aatattattt atgccaaggc gactgataaa atcagagaag
    10681 aagttgcaac aggacagcaa atgtttactg ctatggaaaa tactcattta tttccaaata
    10741 tggtaattca aatggtagca attggtgagg aatcaggtgc ccttgaaaaa atgttaagca
    10801 aagtggctga tttttatgag gaagaagtaa ataatgctgt agatgccctg agtagtttat
    10861 tggagccgat aattatgtca atattaggta tactggttgg gggattggtg gtggctatgt
    10921 atttacctat tttcaaactg ggttctgctg tttaaacaga tcctatttaa tttaatcata
    10981 caatataggc aaacgtgtca atgataaatg cactaataat caattatcca tggtttatgt
    11041 atttagtcgt tgggctcttt tctctagccg ttggaagttt gcttaatgtg ataatctatc
    11101 gcttaccaat tattttgcag gaagagtgga aagagcaatg ctgtgagcta ttccattttg
    11161 aacagcgaaa agaaaaaata aaacttaatt tatttttacc acgttctttt tgtcctcatt
    11221 gtaaagcgat ggttaaagcg tggcagaata ttcctctgtt aagctatatt ttgttaagag
    11281 gacgctgtta ccaatgtgat agtccaattt caatccgcta cccttttgtt gagacgttga
    11341 ctacagtttt atccttatac gccagttggc attttgggtt tacaatacaa ttattatttg
    11401 ctttattagc tatctggata ttaatatcac ttgtttttat tgatcttgat catcaattac
    11461 tccctgatag cctcacttta gggctcttat ggattggcct tattgctaat acccagaatg
    11521 tatttgttag tcttgacgtt gctgtattaa gctgcgctgg cgcctattta gccttatggt
    11581 tattcattaa tctgttttat ctgatgacag gtaaagtagg tatgggacat ggggatttca
    11641 aattatttgc agcatttggt gcctggcttg gatggatgta tttaccatta attctactaa
    11701 tatcttcaat aacgggggct atcattggtt tgatttattt aaagataaat ggtaaatcaa
    11761 gagatactgc aataccattc ggcccttttc tttgcatttc tggattaata gctatgtttt
    11821 ggggagactc aattatcaat tggtacatag gttattggat gtaaaatgtt gattagctta
    11881 gcgcaaccca acaataaata gagaccacac ccctaaaata acatgagaaa cagatacccc
    11941 caaaagagat ttctgtctgt gaaataacca cccccaaaaa agtccggcga taaaagttaa
    12001 tgaggcaaag gttaacccca agtgagtgtg catagttgca aagattagat tggacagaaa
    12061 aattccattc cattgacgaa ataatttttc cccgggtaaa aaatgaaaaa aagtagattg
    12121 taacgcactt ctggccatta gctcttgaat gggggcaaaa aggctatata aaaaaatcat
    12181 agcaaagtaa tattcccaat ccacttcatt atttaccaaa aagcgttttg ttcctgaaaa
    12241 tataggggta ttgtccagat gatcgatacc tattataaaa aaaaccttga taattagtac
    12301 aaaaagcata ataggaattg taaaagctaa tgcctccaag atggtttttc cccaatcttt
    12361 cgcagtgata ccaaatctgg aatatgggag gccagtaaag tgcattgcaa tgagaattgc
    12421 tacagcaaag attgagatta agcagattga tagtacggta gtgacagtta cctgatgtat
    12481 tacagtaacc aggaaatgta gagataaagt atagaggcaa ataatccata gcagagctat
    12541 gataaatacc cccaatgtat tgcgccctga tgcttcttta agagcttgct gcatattttt
    12601 tacagttact tcatttgtaa aacgaagtct cctggaaagt atttgagcca tatttttaat
    12661 taacatcaag ttaagctttg gtttagcttt taccacttct aatggatatt ctataacact
    12721 aagatcagtt ttagccttta ctgacgcaga acgtcgacta ttttctatga gagccatttc
    12781 tcctacaaca tcatcttttg ataaggttgc aagaatatgg atattatgct catcaaactc
    12841 atcgatagtt tttttaaata tttcggcaac accatcgatt ataataaaga atgagtcagc
    12901 gagatcattt tcattataaa caatgcttcc agcagtatag tgcttatgct gaccaatttt
    12961 tgaaaaactg gcaagttcat cttttgagaa accaatcgtt atcgattggt aacttcctat
    13021 taattcagta tccaattgat tggagttgct attatctttt ttagaagcat cattttggtt
    13081 cattgctatt tcaatcctaa atatgcaagt ccacatttac tctatttaat gcagcgacaa
    13141 tatatacctc ataaataatt tttaa
//
LOCUS       DBNCWK010000086        13071 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.086, whole genome shotgun sequence.
ACCESSION   DBNCWK010000086 DBNCWK010000000
VERSION     DBNCWK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.086"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..4932
                     /locus_tag="AC06IO_002035"
     CDS             <1..4932
                     /locus_tag="AC06IO_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HIO9875211.1"
                     /translation="GAISVVADEDNLPRGNNDAASGDAAQSNLTGTLPVNFGADGAGS
                     IDFQGMHGLSTVIGNDNITYNWNAGTNTLTAYRTGGTLGVNDVFKVVVNPTTGQYTFT
                     LLAAINHHAVADNTEGLVDPFVNLNYRVIDGDGDTAIGTLKVTIDDDIPKAFTPEEGF
                     VTNQAGIVRTFDLDFDANIDNNVGADQLGTIAFSGITNGQLVTGTVDGVPNQTLTSGG
                     SAIHYYVSGNNVVEGWINGGPGDVGSTIVFRTTLQPDMNYNASNDTYKFELFQPISTS
                     TNVSITNFNGVNATSREFIYLENASAPGEDILFSGYIRNDNGSFTDATVNTSTQGIGV
                     NNQNMNDRENLRLDFVRNASTNGTTSNMTYEYDDHYTVNNFSFKIVQVTGNPPAGSIE
                     VWVRAYNADDDDPTNNTVSSANNLAHQDALRDDPQVALTQILVNGVPVTPTAVNASGG
                     YLISGLNLNDTITLRSANGYDRVEIENARSGAHGVSNSSLNGETFDIGLFSYNTIKTT
                     PSEININMGLSLTDSDGDKITSSIEINLAPSVFKVGENVDDTSSSNVLHRVGGDTGVV
                     DGSGGADILVGDVGGVEIVGTTARIAFVLDESGSMGQNFGGTTRLEVLKQTMTDILTE
                     LSNTPNASITVHLVKFASVVNGTGTFEITGGELQQALDFISGLQIQQGLLAGTNYEAA
                     LGQTLQWYNSQSGTADVQQTLFFTDGAPTFYMDGNSTEYTNIARVYGNGSQTEEVLWE
                     NLFGEHSGGQATSDRINNLTESDPRDLDGLQSYSVDTNGDGIFETQSVNSRSSGTTQT
                     TNDYVRSIADTFNEVDALQAYGPLRAVSIADNFSVYLQEIDSTGQPYIADSPEVLQDI
                     LDELNPFNVLLAAGSDTIQANEENDLIFGDVLFTDKLAEDEGLDLPKGSGWAVFEELE
                     ANHGWSRQDTLDYIKNHADELGRETVLSSGNKRSGGHDTIGGGQGNDQIYGQEGNDVI
                     DGGSGNDVIVGGTGNDTLTGGSGADQFVLVKGQGGASTGVAPIDTITDFEVNIDKIVI
                     NGNNITGVSVSTPVSNTYTITVNYSDAATEHFKVTLSNGALLNDSGKTHLNGVVISGG
                     TATIDGTIVGAVLYLDLNANNQEDEGERLGITNQYGHVEWVVDLSKFDVNGDGQYVIG
                     EARAVQTGGFDIDTGLSYEINLYGPVGSAVISPLTSLLQAQLEAGMDYETANAALVAR
                     LGLPEGTQIISFNPITGSGEVLAQNAGVMTAAIQFAEIAAIHYSTDESHVSFAVFEAI
                     SKALLELPEGVVADFGDEGFLQSISNYLGLDALVTSDMIDYMAAGQKALEASILTLAP
                     GEDALTAISKIQHVTQGIYAQAIEMALIGYLPLDALKNMSAVLKAYADGDITDEQLNS
                     FEDKLSAVVVNWEDNNVTDTSHQASLQTDRTVDDASVKSEVNHEMAQEVNAVINDFMA
                     EHNISLYYYAQQAQMPQEAQEQQPTEDQAEETVMHDDVVQDLLLDDHSLTMLANNPQL
                     NGGNGNDVLHGTTGNDFIRGGQGNDTMTGGGGVDTFFWLSGDDDGGVDTITDFKANPV
                     DKSSDASVLNLSDLLSDADLETNSLDNYLNVSTTEEGDTAIKVDPNGNGNFDAPAQTI
                     ILEDVDLTAVFATNNSHDIVNQMIANGNLVVEQ"
     gene            5111..5710
                     /gene="wrbA"
                     /locus_tag="AC06IO_002036"
     CDS             5111..5710
                     /gene="wrbA"
                     /locus_tag="AC06IO_002036"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIO9875212.1"
                     /translation="MSNPYILVLYYSRTGSTAQLAQYIARGVESVSGLEARLRTVPPV
                     STTCEAVDKPVPDAGAIYVSLDDLRHCHGLALGSPTRFGNMAAPMKYFWDGTTSLWLG
                     GDLVNKPACVFSSSASMHGGQETTLISMMIPLLHHGMILLGVPYTEPALTSTQSGGTP
                     YGVTHVAGGDNENPLSQDEISLAKALGERLGRVAMGLKF"
     gene            5819..6169
                     /locus_tag="AC06IO_002037"
     CDS             5819..6169
                     /locus_tag="AC06IO_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIO9875213.1"
                     /translation="MVPADAKLLKQAKSLRKRSTDAENCLWYYLRAHRLKGYKFKRQV
                     PIGNYIVDFVCIQRKLIVELDGGQHLLNKEYDKDRSAYFSTLSYKVLRFWNDDVLLRT
                     GDVLESIVRCLEES"
     gene            6225..7007
                     /locus_tag="AC06IO_002038"
     CDS             6225..7007
                     /locus_tag="AC06IO_002038"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIO9875214.1"
                     /translation="MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLI
                     PEELYFPRDYFCDYFDAEKKGYSGVAIYSKYKPVRIVKGLGFDYCDKEGRYIQFDYPK
                     LSVISLYLPSGTSGEDRQEVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLK
                     NWRGNQKNSGFLPEERAWMDELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGW
                     RIDYQVITPGLTEYVADSRIFREARFSDHAPLMIEYKGDWCV"
     gene            7000..7770
                     /gene="xth"
                     /locus_tag="AC06IO_002039"
     CDS             7000..7770
                     /gene="xth"
                     /locus_tag="AC06IO_002039"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIO9875215.1"
                     /translation="MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDENFP
                     VVSFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTDIPELEDPQRRILAATVKGIRLI
                     NLYVPNGGELTSDKYQYKLDWLNKVTRFIQHEISIYSKVAVVGDFNIAPDDRDVHDPV
                     EWEGCVLVSPPERQAFMKILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDH
                     VLLSHELNALCRQSKIDKEPRKAERPSDHAPVWVELEL"
     gene            7907..8134
                     /gene="rpmE"
                     /locus_tag="AC06IO_002040"
     CDS             7907..8134
                     /gene="rpmE"
                     /locus_tag="AC06IO_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIO9875216.1"
                     /translation="MKASVHPDYQTVKVTCSCGEVFETRSTLCKDLNIEVCSMCHPFY
                     TGKQKLVDTGGRVQKFRDRYNMRTGQAKSKE"
     gene            8261..9496
                     /locus_tag="AC06IO_002041"
     CDS             8261..9496
                     /locus_tag="AC06IO_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIO9875217.1"
                     /translation="MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGV
                     AAPVLAIAEAPENAYRFTSKGNLVAVMTNGTAVLGLGNLGPLASKPVMEGKAVLFKRF
                     ADLDVFDIEIDAEDPQSFIATAKRIAPTFGGINLEDIKAPECFEIEQALIEQLNIPVF
                     HDDQHGTAIVVAAGLLNALELQKKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNML
                     LLDTKGVIHSGREDLNPYKFAFARNTSCRTLEDALVDADVFIGVARPDLLNAHLLSLM
                     APNPVIFALSNPDPEIKPELAHSVRKDLVMATGRSDYPNQVNNVLCFPYIFRGALDVR
                     ATCINQAMQIAAVEAIRQLVHEPVPQVVKDNYPGVVNWDFGPDYIIPKPIDPRLKERV
                     PAAVAKAAIASGASQFNNL"
     gene            9707..10999
                     /locus_tag="AC06IO_002042"
     CDS             9707..10999
                     /locus_tag="AC06IO_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875218.1"
                     /translation="MHTENRTNYAMIGWLLCGLGAIFYSYEYLLRIAPSVMENALRTH
                     FNLSASGFGFLSSIYYLAYVPMQLPVGVLLDRYGPKRLLTMACLVCVIGTYMFTGTTI
                     FWVAATGRFLVGFGSAFAFVGVLKIATLWLPENRLAMISGMTSALGPIGAMLGDNFLE
                     LFIVKMGWVKTLNMTAAFGIVLTLVLWVGIRDRKGHHRQSGTVPTLKKGLVDLGIIIT
                     SRQIWVNGMYGCLVYLPTTVFAELWGIPYLHHAHGLTTQAAGLANSVLFLGFIIGAPI
                     MGYISDRLYRRKFPMLIGASGAAIVMMMILYLPGLNESNIQALMFLLGLLYSAQAIVF
                     AVGRELSPGEAAGTAMAVTNMIVMLGAMFLQPLVGHLLDFSLSTHLSDTTGIVVDNMS
                     KLYTVDDYQFALSIIPLGILIAAILTFFLKETHAKAPK"
     gene            10980..12257
                     /locus_tag="AC06IO_002043"
     CDS             10980..12257
                     /locus_tag="AC06IO_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875219.1"
                     /translation="MQKHLNNMTTKKQISYGVLVCFIGAVFYCYEFILRIVPGALQTE
                     LSTALGHISATTFGQISALYYFAYSPMQMPVGMLMDRYGPRRLLTFACLCCALGSWMF
                     TLTWSIVLVGIGRFLVGFGSSFAFVGVLSLALHWLPRRYFSLVAGLITTLGMLGLVYG
                     EIKITEWSEVIGWDHVLMHIAMIGSALCILTFFVVRDGPEGYQPNKYPWPEFFHNVLK
                     VLTSHEVWLIGFVGACLYTSLSVFGELWGKTYLEQAHHLTKVEAAKTVSAVFLGWAVG
                     APLAGYISDVTRRRLLPLVIGAIVSLICISFVLYCPGLSYLTLNILLFLYGVFSATEI
                     IVFIMAKECSGAKLSGTVFAVTNMIISLAGAIFQPLVGKLLDTFGDSGIVAGEHIYTV
                     DDYQVALSILPISLLLVTILAFFIKDKDHSNNS"
     gene            complement(12286..>13071)
                     /locus_tag="AC06IO_002044"
     CDS             complement(12286..>13071)
                     /locus_tag="AC06IO_002044"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HIO9875220.1"
                     /translation="GSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMNTSYSSYLLAES
                     NSDLVNIYRILQLNGESFIDYCQQYFTPEANCKEKYYELRSEFNNLMDSNQKSAVFLY
                     LNRHGYNGLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQFIHGDFRNTFAL
                     AKPGDVIYCDPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETASKGIPVIISNHD
                     TEFTRYHYKKAQIKSFYVPRWISCRSQMRLPVKELVAVFNCPS"
BASE COUNT         3677 a         2216 c         3051 g         4127 t
ORIGIN      
        1 ggtgccataa gcgtagtagc tgatgaggac aatttaccga gaggtaataa tgacgcagct
       61 tccggagatg ctgctcaaag caatttaact ggtacattgc ctgttaattt tggtgctgat
      121 ggtgcaggta gcattgattt tcaaggtatg catggtctga gtactgtcat aggcaatgac
      181 aatattactt ataactggaa tgctggtacg aatacactga cagcctatcg aacaggagga
      241 accttagggg ttaacgatgt gtttaaagta gtagttaatc cgacaacagg tcagtatacg
      301 tttactctgc tggctgctat taatcatcat gctgttgctg ataataccga aggattggtt
      361 gatccatttg taaatcttaa ttatcgagtt attgatggcg atggggatac tgcaattgga
      421 actctaaaag taacgattga tgatgatata cctaaagcat ttacaccgga agaaggtttt
      481 gtaacgaatc aagctggtat tgtaagaaca tttgatctgg attttgatgc caatattgat
      541 aataacgtcg gtgctgatca actcggtaca attgcttttt caggtatcac taacgggcaa
      601 cttgttacag ggacagttga tggtgttcct aatcagactt taacctctgg agggtctgcc
      661 attcactatt acgtgagtgg taataatgtg gttgagggat ggattaatgg tgggcctggt
      721 gacgtgggtt ccacaattgt gtttaggact actttgcagc cggatatgaa ctataacgca
      781 agtaatgaca cctataaatt tgaattgttc cagcctatta gtacatcaac gaatgtgagc
      841 attacaaatt ttaatggggt taatgcaact agtagagagt tcatatatct tgagaatgct
      901 agtgcccccg gagaagatat attattttct ggttacatac gaaacgataa tggcagcttt
      961 actgatgcta ctgttaatac aagtactcaa ggtataggcg tcaataatca aaatatgaat
     1021 gatcgcgaga atttgcgcct tgattttgtc aggaatgcct ctactaatgg cacaacctcc
     1081 aatatgactt atgaatacga tgatcattat accgttaata actttagttt taaaattgtt
     1141 caggttacag gaaatcctcc ggcaggatca atagaagtat gggttcgtgc ttataatgcg
     1201 gatgatgatg atccaactaa caatacagtt agttctgcaa ataatttagc gcatcaagat
     1261 gcattgagag atgatccgca agtagcgctc acacaaattt tagtgaatgg tgtgccggtt
     1321 actccaactg cggttaatgc tagtggtggg tatttaatta gcggcttaaa tttaaatgat
     1381 acgataacac ttagatcagc taatggatat gatcgcgtag agatagaaaa cgcacgttcg
     1441 ggagctcatg gagtttcaaa ctccagttta aatggtgaaa catttgatat tggtttattc
     1501 tcatacaata caattaaaac gactccttct gaaatcaata ttaatatggg tctctcattg
     1561 acagactcag atggtgataa aataacttct tcaatagaaa ttaatctggc tccttcagta
     1621 tttaaggtcg gagaaaatgt cgatgatact tccagctcta atgttcttca ccgcgtagga
     1681 ggtgacactg gtgttgtcga tggttctggt ggcgcggata ttttggttgg tgatgttggc
     1741 ggtgtagaga ttgttggtac tactgctcga attgcttttg tattggatga atcaggtagt
     1801 atgggtcaaa atttcggagg aacaactcgt ttagaagtac taaaacaaac gatgactgat
     1861 atattaactg agttaagtaa tacgcctaat gccagcatta cagttcattt agtcaaattt
     1921 gcatcagtag tgaatggtac aggcactttt gaaattactg gtggagaatt gcaacaggct
     1981 ttagacttta tatctggatt acagatacaa caaggtttat tagctggaac aaactatgag
     2041 gctgctttag ggcaaacctt acaatggtat aatagtcagt ctgggacagc ggatgtgcaa
     2101 caaactctat tttttactga tggcgcccct actttttata tggatgggaa tagtactgaa
     2161 tatacgaata ttgctcgtgt ctatggaaat ggaagccaaa cagaggaagt tctctgggaa
     2221 aatctattcg gtgagcactc tggaggccaa gccacatcag atcgcattaa taatctcaca
     2281 gagtctgacc ccagagattt ggatggtctt caaagctatt ctgttgacac taatggtgat
     2341 ggtatttttg aaactcaatc ggtaaattca cgtagttctg gaacaacaca aactacaaat
     2401 gactacgtaa gaagtattgc agatactttc aatgaagtag acgctttgca agcgtatggt
     2461 cctttgcgtg cggtttccat agcggataat tttagcgttt atttacaaga aatagatagt
     2521 acaggccaac cttatattgc tgactctcct gaagtgttac aagatatact ggatgaatta
     2581 aatccattta atgttttgct tgcagcgggt agtgatacca ttcaagctaa tgaggaaaat
     2641 gatttaatct ttggggatgt gttattcact gataaactgg ctgaagatga aggtcttgat
     2701 ttacctaaag gatcaggttg ggcagtattt gaagagctgg aagccaatca tggatggagt
     2761 agacaagata ccttggatta tataaaaaat catgctgatg aacttgggag agaaacagta
     2821 ctctctagtg gtaataaacg ttctggtgga catgacacca ttggtggtgg ccagggaaat
     2881 gatcaaattt atggccagga aggaaatgat gtcatagatg gtggttctgg aaacgatgtc
     2941 attgtgggtg gaacaggtaa tgatacgctc accggtggct caggtgctga tcaatttgtg
     3001 cttgtgaaag ggcaaggagg tgcgagtaca ggtgtggccc cgatcgacac tattacagac
     3061 tttgaagtga atattgataa gattgttatt aacggtaata acattactgg tgtcagtgta
     3121 tccactccgg tttctaacac ttatactata acggttaatt attctgatgc tgctacggaa
     3181 cactttaaag tgacattatc caatggtgct ttattgaatg actctggaaa aactcatttg
     3241 aacggagtgg ttataagcgg tggcacagct actattgatg gcacaatcgt tggcgccgtt
     3301 ttgtatctgg atctgaatgc caataatcag gaagatgaag gggaacgact gggtattacc
     3361 aatcaatatg gtcatgtgga atgggtcgtt gatctctcta aatttgatgt caacggtgat
     3421 ggtcagtatg ttataggcga ggctcgtgct gttcaaacgg gtggttttga tatagatacg
     3481 ggtttaagtt atgaaattaa tctttacggg ccagtaggtt cagcagtcat atcaccactg
     3541 acaagcctct tgcaagctca gttagaagcc ggtatggatt atgagacagc taatgctgct
     3601 ttggtcgcgc gcttgggact gcctgaggga acacaaatta tcagtttcaa tcccattact
     3661 ggcagcggtg aagtcttggc tcaaaacgca ggtgtaatga ctgcggccat tcagtttgct
     3721 gaaatagccg caatacacta ttcaaccgat gaaagccatg tttcattcgc tgtctttgag
     3781 gcaattagca aggcattgct tgaacttcct gaaggggtag tcgctgattt tggcgatgaa
     3841 ggtttcttgc agtccatttc aaattatctg ggattagatg ctctggttac ctctgatatg
     3901 attgactata tggcagctgg ccagaaagcg ttagaggcca gtattttgac tcttgcgccc
     3961 ggagaagatg ctctgactgc catcagcaag atacagcatg tgacccaagg tatatatgcc
     4021 caagctattg aaatggcatt gatagggtat ttgccattag atgctcttaa aaacatgagt
     4081 gctgttctta aggcttatgc tgatggagat attacggatg aacaactcaa ttcttttgaa
     4141 gacaagcttt ccgctgtggt tgtgaattgg gaagataaca atgttacaga cacatctcat
     4201 caggcttcat tgcaaacaga tcgtactgtt gatgacgctt cagtcaaatc agaggtgaat
     4261 catgaaatgg cgcaagaagt taatgccgtg attaatgatt ttatggctga acataatatt
     4321 tctttgtact attatgctca acaggctcaa atgcctcaag aagcacaaga acaacaacca
     4381 acagaggatc aggctgaaga gacggtcatg catgacgacg ttgttcaaga tttgttgctt
     4441 gatgatcata gccttaccat gttagctaat aatccccaat taaacggtgg taatggcaat
     4501 gatgttctgc atggaactac cggaaacgat tttataagag gcggtcaagg aaatgatacg
     4561 atgacaggtg gtggcggtgt tgataccttc ttctggttaa gtggcgacga tgatggtggt
     4621 gtggatacca ttaccgattt caaagcaaat cctgttgata aatcatcaga tgccagcgtc
     4681 cttaacttaa gtgatttgct atctgatgcg gatctggaaa ccaatagttt ggataattat
     4741 ctgaatgttt ctaccacaga agagggtgat acagccatta aagtagaccc taatggtaat
     4801 ggaaactttg acgctccagc gcaaacaatt atattagaag acgtggattt aacagctgtt
     4861 tttgctacca acaattcgca tgatattgtg aatcaaatga tcgctaatgg aaacttggta
     4921 gtagaacagt aagatgaact cattacattg gtgaatatgg aattcaccaa tgttttatta
     4981 attggaaaat atcctcgcga catttttctc gctgctatat actcatgtgt tcattcgata
     5041 ataagccttg caattcattt cacgttatta aattgatgaa aaaaggcaaa aaacgctatg
     5101 gagcagtatc atgtctaatc cttatatctt ggttttatac tattctcgca ctggctcaac
     5161 ggcacaatta gctcaatata ttgctcgtgg ggtggaaagt gtgtctgggc ttgaagccag
     5221 gttacgcact gtacctcctg tatctaccac ttgcgaagcg gtggataagc cagttcccga
     5281 tgctggcgca atttatgttt ccttggatga tttgcgtcat tgtcatgggt tggctttagg
     5341 tagccctacc cgctttggta atatggctgc tccaatgaag tatttttggg atggaactac
     5401 ttctttgtgg ctgggtggag atctggtgaa caaaccggct tgtgtgtttt cttcttcagc
     5461 gagtatgcat ggtggacagg agactacctt aatctccatg atgattcctt tgttacatca
     5521 tggcatgatt ttattaggtg ttccttatac tgaacctgcc ctaaccagta cgcagtctgg
     5581 tggtacgcct tatggtgtaa cccatgtggc tggtggtgat aatgagaatc cattaagcca
     5641 agatgagatt tcgttggcta aagctctggg ggaaagattg gggcgtgtag caatgggact
     5701 gaagttttga gatggtttgg atgtttattt cccctctccc cacccctctc ccgcgaaaga
     5761 aattgtataa aaaaatttgg gtatgattcg cgggagaggg gtggggagag gggaataagt
     5821 ggttccagct gatgctaaat tattgaaaca agcaaaatct ctccggaaaa ggagcactga
     5881 tgctgaaaat tgtttatggt actatctaag agcacatcga ttaaaaggat acaaatttaa
     5941 aagacaagtt cctattggga attatattgt cgattttgtt tgtattcaga gaaaactcat
     6001 tgttgaactg gatggtggac aacatctttt aaataaagag tatgataaag accggagcgc
     6061 ctattttagc acattaagtt ataaggtttt gcggttttgg aatgatgacg tgttattgag
     6121 aactggggac gtacttgaga gtattgtcag atgcttggaa gaatcataag gttgataaga
     6181 ttaacattta actgttcagt aatgacagcg gagtacccta agtaatgaaa gtaattagtt
     6241 ttaacgccaa tggcatacgc tcttctgccc gtaatggatt ttatgattgg cttgtagggc
     6301 aggatgttga ttttgtatgt attcaggaaa ctaaatcgca agtagagcaa ttaatcccgg
     6361 aagaacttta ttttccaagg gattattttt gtgattattt tgatgctgaa aagaaaggct
     6421 acagtggggt tgccatttat tcaaaatata aacccgtgcg tattgtgaag gggttggggt
     6481 ttgattattg tgataaagaa gggcgttata tccaatttga ttaccctaaa ttaagtgtga
     6541 tttctcttta tttgccctct ggaacaagtg gtgaggatcg gcaagaagtg aaatatgctt
     6601 ttctggagca atttgccaga cacttgatga atttaaaaaa tgagggaaga gagttaatta
     6661 tttgtggtga ttacaacatc gcgcataaaa agatagattt aaaaaattgg agagggaacc
     6721 agaaaaactc aggattttta cccgaagaac gtgcctggat ggatgagtta tttggttcta
     6781 tggggtttgt agacgctttt agggtacata atcaggaaga agagcaatat acctggtggt
     6841 cttatcgagc tagagcctgg gaaaagaatg tcgggtggcg gattgattat caggtgatta
     6901 cgcctggtct gactgaatat gtcgcggaca gccgaatttt tcgcgaagca cgtttttcgg
     6961 atcatgcccc tttaatgatt gaatacaagg gagactggtg tgtttaaact cgctagctgg
     7021 aatgttaatt cattaaaagt ccgtctggaa caggttttgc aatggcttga gtcttcacaa
     7081 gttgatgtgc ttgccatgca ggagactaag ctgactgatg agaactttcc tgttgtttca
     7141 tttacagaca aagggtacca cgtcattttt tcaggtcaga aaacttataa cggggttgcc
     7201 attattagtc gatatcctat gacagatatt caaaccgata ttccagaatt ggaagatccc
     7261 cagcggcgta tcctggctgc tacggtaaaa ggaatcagat tgattaatct ttatgtgcct
     7321 aatggtggag aactgacttc agacaagtac caatataaac tggattggtt aaataaagtg
     7381 actcgtttca ttcaacatga gataagcatt tattctaaag tagcagttgt gggtgatttt
     7441 aatattgcgc ccgatgatcg ggatgtccat gatccagttg agtgggaagg ttgtgttttg
     7501 gtcagccctc ctgaaaggca agcatttatg aagatacttg aattgggttt acatgacagt
     7561 ttccgtaatt ttgctcagga agaaaaagcg tttagctggt gggattatag agccgcagca
     7621 ttccgacgta atcgtggttt gcgcattgat catgtgctat tgagccatga attaaatgct
     7681 ttatgtcgac aatccaaaat cgataaagaa ccaagaaaag cagagaggcc ttccgatcat
     7741 gcgccggttt gggttgagtt agaattataa atgtctgagg tgtttgagaa aaaattgctt
     7801 tatgagatca tttctgctat tgttttgact catttgtgta gagtatggtg cttggcaata
     7861 gcgtccaggt ggcgatactt atttgaaact agaagagagt ggtattatga aagcatcagt
     7921 tcatcctgat tatcaaacag ttaaagtaac atgcagctgt ggtgaagtat ttgaaactcg
     7981 ttctactttg tgtaaagatt tgaacataga agtttgttca atgtgccatc ctttttatac
     8041 aggaaaacaa aagttggttg atactggtgg ccgtgttcag aaattccgtg acaggtataa
     8101 tatgcgtact gggcaagcca aatcaaaaga ataatatttt aatttttgag tttttaacaa
     8161 aggcacaaat tttttgtgcc ttttctctaa gagaggatta aaaattcaaa tttttgatat
     8221 gatgttttca ccgcgtgatc tgaaatagag agtatgcact ttggataatg aagtaataaa
     8281 gcagcgggca ttggattatc atgagttccc tgttccaggg aaactctctg ttcatcttac
     8341 aaaatcgact aattcccaag acgatttgtc tttggcctat acacctggtg tagctgcccc
     8401 agttttagcc atagctgaag cgccagaaaa tgcttatcgt tttaccagca aaggcaatct
     8461 ggttgcggtg atgaccaatg gcactgcagt gttaggtttg ggaaatcttg ggcctttagc
     8521 gagtaagcct gtgatggaag gcaaagcggt attatttaaa cgatttgctg atcttgatgt
     8581 ctttgatata gaaatcgatg cagaggatcc tcagtctttt attgctactg caaaacgcat
     8641 tgccccaact tttggcggca taaatcttga agacatcaaa gctccggagt gttttgaaat
     8701 agaacaagct ttgattgagc agttaaatat tcctgtattc catgatgatc agcatggtac
     8761 tgcgattgta gttgcagctg gtttgttaaa tgctttggaa ctacagaaaa agaaattaag
     8821 tgacattaaa attgtctgca tgggtgctgg agctgctggt attgcttcta tgcgactcct
     8881 tgtggcatta ggggccccaa aaagtaatat gcttcttctg gacaccaagg gagttattca
     8941 ttcaggccgc gaggatttaa atccctataa gtttgctttt gccagaaata cttcctgtcg
     9001 tacacttgaa gatgctttgg tcgatgccga tgtgtttatt ggcgttgcaa ggccagattt
     9061 gctgaatgct catttattaa gtttgatggc gcccaatcca gtgatttttg ctttatctaa
     9121 ccctgatcct gaaatcaaac ctgaattggc tcatagtgta cgtaaagatc tggtgatggc
     9181 aacaggacga agtgattatc ctaatcaagt taataatgta ttatgttttc cttatatttt
     9241 tcgtggtgcg ctagatgttc gtgccacttg cattaaccag gccatgcaaa ttgcagctgt
     9301 tgaagccatt cgtcaattgg tgcatgagcc tgttcctcag gtagtgaagg acaattaccc
     9361 aggagttgtg aattgggatt ttggacctga ctatattatt ccaaagccta tcgatcctcg
     9421 cttaaaagag agggtccctg ctgctgtagc gaaagctgca atagcgagtg gcgcaagtca
     9481 atttaataat ttataaggta ataaattcac cttgtagaag taagcagttt aggtcttaat
     9541 agcgtttaaa cgaaggatat cttggaaaat tctttgtaaa gccaccagct ggcttgcaga
     9601 gagattgcaa gatgagcttt tatgcgctta caagatatgc tgggatgcag aaatgggaac
     9661 taagacagga tttaaaaata cttgactaat tgttaaggag ataattttgc atactgaaaa
     9721 ccgaactaac tatgcaatga taggatggct actttgtggg ctgggagcaa tattttatag
     9781 ttatgagtat ttgttacgca tcgcaccaag cgtaatggaa aacgcgctaa ggactcattt
     9841 taatttatcg gcgtctggtt ttggcttcct ttcttccatt tattatctag cttatgtccc
     9901 tatgcaattg ccagttgggg ttttattgga caggtatggg ccgaaaagac tactaacaat
     9961 ggcttgcttg gtatgcgtta ttggaacgta tatgtttacg gggacaacta ttttttgggt
    10021 agccgcaaca ggtcgttttc tagttggatt tggctctgct tttgccttcg tcggtgtttt
    10081 aaagatcgca actctttggc tgcctgaaaa tcgattggct atgatctctg gtatgacgtc
    10141 ggcactaggc cccatcggtg ctatgctggg cgataacttt ctggaattat tcatcgttaa
    10201 gatgggttgg gttaagacac ttaacatgac cgcagctttt ggtattgtat tgactttagt
    10261 tttgtgggtt gggatcaggg acagaaaggg acaccacagg caaagcggta cagttcccac
    10321 tcttaagaag gggttagttg atctgggaat tattattacc agccgacaga tatgggttaa
    10381 tggaatgtat ggttgtttag tttatctgcc aactacggtt tttgcagagt tgtggggtat
    10441 tccctattta caccatgccc atggcttaac tacccaagcg gcaggattag ctaactctgt
    10501 actatttttg ggatttatta ttggtgctcc aataatggga tatatttcgg atcgtttgta
    10561 tcgacgtaaa ttcccaatgc taattggtgc tagcggcgct gcaattgtca tgatgatgat
    10621 tttatatttg cctggattaa atgaatccaa tattcaagct ttaatgtttt tattaggatt
    10681 gctttatagc gctcaagcta tagtttttgc tgtgggcaga gagttgagcc ctggtgaggc
    10741 agcaggaacg gcaatggcag taactaatat gatagtcatg ctcggcgcaa tgtttttgca
    10801 gccgttggta gggcatttat tagactttag cttatcaact catttaagtg atactactgg
    10861 tattgttgtg gataacatga gtaaactata taccgtagat gattatcagt ttgccttatc
    10921 cattattccg ttaggtatat tgatagcagc cattttaact ttctttctaa aagaaactca
    10981 tgcaaaagca cctaaataat atgactacta aaaaacaaat ttcatatggc gtcctggttt
    11041 gctttatcgg ggctgttttt tactgttatg aatttattct cagaattgtt ccgggagctt
    11101 tacaaacgga gctaagtaca gctttgggac atatttctgc taccaccttt ggacaaattt
    11161 cagccctgta ttattttgca tattcaccca tgcaaatgcc tgtagggatg ttaatggatc
    11221 gctatgggcc aagacgactt ttgacttttg cctgtttgtg ttgtgccttg ggctcatgga
    11281 tgtttacttt gacctggtca attgtgcttg ttggaattgg tcgattcctg gtgggatttg
    11341 gctcatcctt cgcttttgtc ggtgtcttat ctctggcttt gcattggctg ccacgtcgtt
    11401 atttttcttt agtggctggt ttgattacta cgctaggtat gctaggactg gtttatggtg
    11461 aaatcaaaat aactgaatgg tcagaggtca taggctggga tcatgtttta atgcacattg
    11521 caatgattgg ttctgcgctg tgtatcttaa ctttctttgt tgttagagat ggtccagaag
    11581 gatatcaacc caacaaatat ccttggcccg agtttttcca taacgtgctt aaggtactaa
    11641 cgtcccatga agtatggctt attgggtttg ttggagcctg cctttatact tcattatcag
    11701 tatttggaga gttgtggggt aaaacctatt tggagcaagc acatcattta accaaagtag
    11761 aagccgcaaa aactgtttct gctgtttttc tggggtgggc ggtaggagct cccttggctg
    11821 ggtacatatc cgatgttacc cgcaggcgat tgttgccttt ggtgattggt gcgatagttt
    11881 cattaatatg cattagtttt gttttgtatt gccctggcct atcctatctt accttgaaca
    11941 tattgttgtt cctgtatggt gttttcagtg caactgaaat tatagttttt attatggcta
    12001 aggaatgtag tggtgccaaa ttatcaggaa cggtttttgc agtaaccaat atgattattt
    12061 ctttggctgg tgctattttt caaccgctcg tagggaagtt actggatacc tttggcgata
    12121 gcggtatagt ggcaggggaa catatctata ctgttgatga ctatcaagtg gctctttcga
    12181 tattacctat ttcattactt ttggtaacta ttctggcttt ctttattaaa gataaggacc
    12241 attccaataa ctcctaatcc tggtaaagac tcttccttgt ctttgtcagg atggacagtt
    12301 gaatactgcg actagctctt taaccggtag tcgcatctga cttctgcagc ttatccagcg
    12361 aggtacatag aaacttttta tttgtgcttt tttgtaatga tatcgtgtaa attcagtatc
    12421 atgattcgat ataatgacag gaatcccttt ggaagctgtt tcctgggcca attcagccag
    12481 ctcaatttga tcttctaaag aaaataattt ttggttatag ggtaatgttt gattgttttc
    12541 ggtcagtggc acataggggg ggtcacagta aatgacatca ccgggtttag ctaaggcaaa
    12601 agtgtttcga aaatcgccgt gaatgaactg tgccgaaaga cattttttat aaaacagcat
    12661 catttcctta cgtggaaaat aaggtttttc gtaaagtcca aaagggacat tatattttcc
    12721 ttttgaattg tatcggcaca aaccattata tccatgccga ttaagataga gaaaaaccgc
    12781 tgatttttga tttgaatcca ttaaattatt aaattcactt cgcaattcat aatatttttc
    12841 tttgcaattg gcttcggggg taaagtattg ctgacaatag tctataaagg attcgccatt
    12901 taattgtaga attctataaa tattaaccaa atcagaattg ctttcagcca gtaggtaaga
    12961 tgaatagctg gtgttcataa acaccacgcc tgaacctgca aatggctcta ttaaacgctg
    13021 gccagtgggt aaagaggaga taatttcatt taaacaattg tacttacttc c
//
LOCUS       DBNCWK010000087        12995 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.087, whole genome shotgun sequence.
ACCESSION   DBNCWK010000087 DBNCWK010000000
VERSION     DBNCWK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12995
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.087"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            148..1386
                     /locus_tag="AC06IO_002045"
     CDS             148..1386
                     /locus_tag="AC06IO_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIO9875221.1"
                     /translation="MNWKEKVKTKFYSCDRFVRFVIQHFIQDDCTYIASALAFTSLLA
                     VVPLMSVGLAIFSSFPVFQGLAEPVQNFIFDNFVPATGKIVQSYLQQFTSQVSKLSIW
                     GIVFLIFTALLVMFTIERAMNKIWRVSSSRHGVSAFLLYWAIISLAPVLLGLSLAASS
                     YLFSMPILADHRAPYTILHYSPFFLSLIGFTFLYVVVPNCPVKIRHAFWGGLVAAILF
                     ESAKHAFAYYLIRYNTYALLYGAFATVPIFFIWVYWVWIITLLGAEISYAFSVHHQRR
                     GGKSLDGFSHALLWLHQLWLAQQHGKGLSFNDLVDASKQPFAVDVDEMINALIYHELI
                     HATADGHYMLSRDLSHVTLYDLTQLLPYRLPTHLELQYSKASLAEQWRAAFKRHNEEL
                     KKSLDINLEELFKKTGTVIK"
     gene            complement(1433..3007)
                     /gene="wipB"
                     /locus_tag="AC06IO_002046"
     CDS             complement(1433..3007)
                     /gene="wipB"
                     /locus_tag="AC06IO_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipB"
                     /protein_id="HIO9875222.1"
                     /translation="MKTQRIIHPNIDIRKFPEVNADFSMTDISMGDLHANALLFLNIL
                     VREGIIAISPENYAKFSEIYTLPEFQADYWGTEAPVFSAENKQERLEEIKKQYNALIA
                     QIKIINTKKLIRLIGDELVDRGVIDYFILKLLQALHDQGADFEILLSNHGIEFVEACE
                     LFNENGNKLVAKRLGNIQHGNSFHALQEAIAAGAISNEEVLNIYHQVYKKHLKIISYS
                     LDPEANEIKVYSHAGIGLNHIRGLARKFKVPYSEESAVDLAKTIDAINKKFAEKASTG
                     EIHTLYTHDMMYRGYAGEYLNSTDEVVAATVWGREYGDLIRTSKKFKVTFIHGHDSYD
                     PEKVEHVTLNDQLGQFQNNVGDLYLYSTNGMRAVPTQCLNPDKKVQSLREKNRPDKPN
                     DYVVKIHHTKPSFFKTTHPKMIFPDSYKRIWDSTPGHSNITKIKALLKDYTKEDSILG
                     SFWGLIFTLHWGRHHVKSVHQIAQTQYTSVEAILSDLKALKPREGGSLDKRIKFIELQ
                     IIAQRRDSPEPQFNLK"
     gene            complement(3206..4531)
                     /gene="hslU"
                     /locus_tag="AC06IO_002047"
     CDS             complement(3206..4531)
                     /gene="hslU"
                     /locus_tag="AC06IO_002047"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIO9875223.1"
                     /translation="MVMTPREIVQELDKHIIGQDDAKRAVAIALRNRWRRMKIKDPVL
                     RNEIMPKNILMIGPTGVGKTEIARRLANLAKAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LTDMAIKQEREFAMKKVEHLAEDAAEERILDVLLPPARGTLTPGEKNTTARQVFRKQL
                     REGELNDNEIEIEVAATPVGIEIMAPPGMEEMTSQLQSMFQQVGSYRTKTRKMTVAKA
                     MKILREEEAAKLINEEDIKLKAIESVEQNGIVFIDELDKIAKRSDTVSGGDVSREGVQ
                     RDLLPLVEGTTVSTKYGMVKSDHILFIASGAFHVAKPSDLIAELQGRLPIRVELSALS
                     VEDFVRILTEPSASLTLQYSALMETEGLTLTFDETGIRRIAEVAWQVNERTENIGARR
                     LYTVMERLLEVVSFEATDKAGETVHVDKAYVDKNLGQLIADEDLARYIL"
     gene            complement(4533..5081)
                     /gene="hslV"
                     /locus_tag="AC06IO_002048"
     CDS             complement(4533..5081)
                     /gene="hslV"
                     /locus_tag="AC06IO_002048"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIO9875224.1"
                     /translation="MEQFHGTTILSVRRGNQVVIGGDGQVTLGNTVMKGNARKVRRLY
                     KDKVIAGFAGGTADAFTLFERFEAKLEMHQGHLIRAAVELAKDWRTDRILRRLEAVLA
                     VADSKASLIITGNGDVIEPEESLIAIGSGGPFAQAAARALMENTQLSAKEIVQKALTI
                     AGDICIYTNNNLTIEELNDEGK"
     gene            complement(5268..6491)
                     /locus_tag="AC06IO_002049"
     CDS             complement(5268..6491)
                     /locus_tag="AC06IO_002049"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIO9875225.1"
                     /translation="MTGRVCVLVMDSFGIGASLDAARYGDAGANTLVHIYEACKRGEC
                     DIEGVRKGPLMLPNLAGKGLYHAAMASSGLPFIDLATLAIPSGYYGYAVEQSLGKDTP
                     SGHWEMAGVPVTFEWGYFPDKPYCFPEELISEFIKQCNLPGVLGEKHASGTIIMDELG
                     EEHIRTGKPIVYTSADSVFQIAAHEEAFGLQRLYDICKIARNLVDKYQIGRVIARPFT
                     GKPGSFKRTGNRKDYATPPPEKTLLDFLKEDGREVIAIGKIADIYAHQGVTQEIKADG
                     NMALFDATLSAMKTAPQGSLVFTNFVDFDSSYGHRRDIAGYAHALEQFDARLPELEVL
                     LQPNDMVFIAADHGCDPTLPGSDHTREHIPVLVFGPQINSKFIGRRDCFADIGQSIAE
                     HLQLSSPLTHGVSFL"
     gene            complement(6488..7771)
                     /locus_tag="AC06IO_002050"
     CDS             complement(6488..7771)
                     /locus_tag="AC06IO_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875226.1"
                     /translation="MQVVDEFKAFDVKASLMPWLVCFAATMFFFYEFIQGNMFASIAA
                     NIMQDFQIQADKMAYLSSIYYLANVIFLFIAGIVLDRFSIKNTILIAMFLCVISTFIL
                     SYSDSFYVALFCRFVTGIGSAFCFLGPVRLASHWFPPQRMALVTGAIVTVAMTGGMLA
                     QYPLTKLVLYVGWRQAVQDVGILGLAMLVLMFFWIKERPQVAIKKEGKPINVLLAARK
                     AYLNAQNLRAALYTSLMNMAIAVFGAMMGTLYLEQRLGISPDQAAMINGMLFFGAIIG
                     SPLMGWISDKVGLRVLPMKGGVLASLLILLGVLYLPVSVTMMAVLFFLLGLFTSAQVI
                     SYALVAESSPPMMTATAVSVVSILTQGGYLIYQNLFSALLMNYGDVRLINGVPVYLLE
                     AYQHAAIILPIGLLIALLLLFGLKETRCQQVEHTV"
     gene            complement(7784..9052)
                     /locus_tag="AC06IO_002051"
     CDS             complement(7784..9052)
                     /locus_tag="AC06IO_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875227.1"
                     /translation="MSHSVAVESKTFVPRGDFMAWVVCLSAGLFFLYEFFQLNIFDVI
                     NQSLREDFHIDATQLSWMSSTYLWADILFLLPAGLILDRFSTRKVILTAMFVCVVGTI
                     GFAVTESFFLASFFHFLSGIGNAFCFLSCVVLVSHWFPPRRQALVIGSLVTMAFIGGM
                     MAHTPFAYLNDLFGWRRALLIDGVVGAFLILWIYMIVQDRPEKSPAHKLTNEGQILTS
                     FMKALSNKQNWLAGLYTSLLNLPIMVLCALWGASYLQVAHHLPDIAASNVVSLIFMGS
                     VVGCPLVGWLSDTQGRRKPLMIFGAIATLITTIPLFINVVLTQMSLSILFFALGLFTS
                     TQVISYPLVAESNQPENTGAATGIASVIIMGGGGVAQVLFGWLMAHHAGTNVTAYTVS
                     DFQFAMWMFPVAAIAGLVAVLMTRETYCKR"
     gene            complement(9103..9732)
                     /locus_tag="AC06IO_002052"
     CDS             complement(9103..9732)
                     /locus_tag="AC06IO_002052"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIO9875228.1"
                     /translation="MAKLYFYYAAMNAGKSTVLLQSSYNYRERGMQTLLFTPAIDTRY
                     QYGTICSRIGLSEQAYAFNNTDNLYVLTQELQLQATKYSCVLIDEAQFLTREQVYQLT
                     EITDQMSIPVLAYGLRTDFRGELFPGSQFLLAWADELIELKTICHCGRKAIMNMRIDE
                     NGQAVVEGEQVLIGGNESYVATCRLHYKRGEAGKTFPRNKLFNKDTNTF"
     gene            complement(9796..11277)
                     /locus_tag="AC06IO_002053"
     CDS             complement(9796..11277)
                     /locus_tag="AC06IO_002053"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIO9875229.1"
                     /translation="MSFQTDKEIQQKFFSYTLFVLVVLLICRLIANYFFPLFDTTESR
                     YAEIARKMLETGNWVTLQQDYGVPFWAKPPLSIWLSAFFMQLLGVNEFAVRLPGLLLS
                     VGVLWLVWGVAKKQSGSMAALFSLLTLAGSLYFFLDAGTVMTDPALLFCITLASVSFW
                     HAMVYENKIWSYAFFAGLGLGLLAKGPIAVVLVGMVVFFWVLLRNEWLNLWKRLPWFK
                     GTFLVLLIAMPWYVLAELRTPGFLNYFILGEHFHRFLTPGWSGDKYGIVHHVPKGTIW
                     LYAIAGIFPWNIVAGGWLVKHGKMLPSLCQSEDGWLSYLTLWMITPLLFFTFASNIIY
                     PYVFPSLPAFALLFAEILNRLNLELKHLKWVLISSLLCGVLFLAVVFVLGVKPDVVPN
                     THKPIITAWLNQNPTAGSHLVYWGYKTDFSAEFYSGGQVKSVKNPSELCKLFSNHLEN
                     YLVINSKEIQQIPGELLAKLTPINKFRVHRDTLILFYSPVMSC"
     gene            11567..12916
                     /locus_tag="AC06IO_002054"
     CDS             11567..12916
                     /locus_tag="AC06IO_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0634 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875230.1"
                     /translation="METNMTFVLKEFDVLKSHFNDTVKIILQREKKDKIEDLPNPRKE
                     ELQFLTAVLNQLEAKIDELKPRSLASYVHVFYGAMLLVCKDVENNLRVMEKKENSLLF
                     TRLMDGMGITDENMPTSEQNIMFYRGLNKFLNFIYESNDSRKGLKKEHFLQVLSLKKI
                     YSLAKLSYEQEEAAENNALSKLTADGKTKANANSFHVEKPIDSSIVEQFKSWDEMKGA
                     LHQLILDELSDKNVAKISALSQARSAQLKFLQTMAEQLDKIPNQSLEPSEKMAILAGA
                     MYIVRGQIAQEYGKDPLSNDKISATVIHTGLSTILHANADCCEDKEVLIAAANKFIRH
                     MVIERPEQSNKKITKESIRENNMFSDIAGFQLVSVLTLIQNMIKTCRTDAIEACVTKR
                     KEELEALKPKKEGYSIASSVTGYVGSWFKKAPSISEEDEEDDLKDQSTAEETSKPTV"
BASE COUNT         4114 a         2795 c         2334 g         3752 t
ORIGIN      
        1 tcattgattt gtcatgggag tttagacagc ctaaatgacc gaattaaaac ttttttaata
       61 atgaatttgg tatttgcata agtcctgact atacaaaaac gggcataaaa cgatatagtt
      121 atacaataaa aatcttgtat ttaatccatg aattggaaag aaaaagtaaa aactaaattc
      181 tactcctgcg atcgctttgt tcggtttgtt atccaacatt tcatacaaga cgattgtacc
      241 tacattgcct cggcattagc ctttacaagc ttactggcag tcgtgccatt gatgtctgtg
      301 ggtttggcaa tattttcttc atttccagta ttccagggtt tggcggagcc agtacagaat
      361 tttatttttg acaattttgt tccagccaca ggtaaaattg tgcaaagcta tttacagcag
      421 tttacctctc aagtatcaaa actctcaatt tggggtatag tctttctaat ttttactgcg
      481 ttattggtca tgttcactat tgaacgagca atgaataaaa tctggagagt gagttcatcc
      541 cgacatggtg tttctgcctt tttactctat tgggctatca tttctcttgc cccggtttta
      601 ttaggtttga gcctggcagc aagctcctat ttattctcca tgccaatatt ggcagatcat
      661 cgagctcctt acactattct tcactattcc ccattttttc tttcattgat tggctttacc
      721 tttttgtacg tggtggttcc caattgtcct gtcaaaatcc gtcatgcttt ttggggtggc
      781 ttggttgctg ccatcttgtt tgaatcagca aagcatgcat ttgcatatta cctgatacgc
      841 tacaatacct atgcgttgtt gtatggggca tttgccacag tacctatatt tttcatttgg
      901 gtttattggg tatggattat tactttgttg ggcgctgaaa tcagttatgc cttttcagtg
      961 catcatcagc gtcgtggagg gaagtccctc gatggctttt cacatgcgtt gctctggctg
     1021 caccagttat ggttagcgca acaacatgga aagggattaa gctttaatga tctggtggat
     1081 gccagcaaac aaccttttgc tgtggatgtg gatgaaatga tcaatgctct gatttatcat
     1141 gaactgatcc atgctaccgc tgatggacat tacatgttaa gccgtgatct cagtcacgtt
     1201 actctttatg atttgactca attgttaccc tatcgcttgc caactcatct ggagctgcaa
     1261 tattccaaag cctctctggc ggaacaatgg agagccgcct ttaaaagaca taatgaagaa
     1321 ttgaaaaaat ctctggatat taatttggaa gaattattca agaaaacagg aacagtaatc
     1381 aaatagtgtc atatatcgaa tgtggaacaa gaataaaaac aaggtaaaag gactatttca
     1441 agttgaattg tggttctggg gaatcacgcc tctgagctat aatttgtaat tcaataaact
     1501 taatgcgttt atctaatgag ccaccttccc ttggcttaag tgctttcaaa tccgagagaa
     1561 tagcttctac tgatgtatat tgggtttgtg caatctgatg cacggattta acatgatgtc
     1621 ttccccagtg caatgtgaat attaagcccc agaaagaacc caaaatactg tcttccttgg
     1681 tataatcttt aagtaacgcc ttaattttgg tgatattgga atgtcctggt gtgctatccc
     1741 atattcgttt gtagctgtcc gggaatatca tcttgggatg agtcgtttta aaaaatgatg
     1801 gttttgtatg atggattttt acaacataat cattcggttt atctggtcga tttttttccc
     1861 ttaggctctg aactttttta tctggattca aacattgtgt tggaaccgct ctcatgccat
     1921 tggtggaata caaatataaa tcacctacat tattttgaaa ttgccctaat tgatcattta
     1981 acgtgacatg ttctaccttt tcaggatcgt aactatcgtg gccatgaata aaggtgactt
     2041 taaatttttt ggacgttcga atcaaatccc catattcacg tccccaaacc gttgcggcaa
     2101 ctacttcgtc tgttgaattc aaatattcac ctgcataacc tctatacatc atgtcatggg
     2161 tataaagcgt gtgaatttct cccgtagatg ctttttctgc gaattttttg ttaattgcat
     2221 caattgtttt agccaaatca acagccgatt cctctgaata gggtacttta aattttctgg
     2281 cgagccctcg aatatgattt aaaccaatac cagcatgact atagacttta atttcatttg
     2341 cttctggatc caatgagtaa gaaataatct tcaagtgctt tttgtaaact tgatggtaaa
     2401 tatttagcac ttcttcattt gaaatcgcac cagcagctat tgcttcttgc aaagcatgaa
     2461 atgaattacc atgctgaata tttcctaatc tttttgctac taatttgttt ccattttcat
     2521 tgaatagttc acaggcctca acaaactcaa tcccatgatt tgaaagtaat atttcaaaat
     2581 cagcaccttg atcgtgcaaa gcttgtaata attttaaaat gaaataatca ataacaccgc
     2641 gatctactaa ttcatcgcct atcaagcgaa tcaatttttt agtattaata attttgattt
     2701 gcgctattaa tgcattgtat tgtttcttga tttcttccaa acgctcctgt ttattttcag
     2761 cgctaaaaac aggtgcttct gtcccccagt aatctgcctg aaactccggc aaagtataaa
     2821 tttcagaaaa tttggcataa ttttcgggag aaatagcaat tattccctcg cgaacaagga
     2881 tattaagaaa aagcaaggca ttagcatgca aatctcccat gctaatatcg gtcatggaaa
     2941 aatctgcatt tacttcagga aactttctaa tatcaatatt gggatggata attctctgag
     3001 ttttcattta tttctgatat aaacaaccat tatgtacata atgatatcag aatataatta
     3061 gcattgcctt gccctttgca aaagtaaatt gcatggttgg acaatcagag tcacatgagg
     3121 gatatctctt tttcgagaaa tatccccaca aaaagttgat atgttaaaaa gatttcattc
     3181 ttgatttcag tttaactatt ctggattaca aaatatagcg agccaaatcc tcatcagcga
     3241 ttagttggcc taaattctta tcgacataag ctttatcaac atgcacagtt tcgccagctt
     3301 tatcagtcgc ttcaaatgaa accacttcca gtaaacgttc catgacggta tataatcttc
     3361 ttgctccgat attttccgta cgctcattca cttgccaggc tacttcagca atacgtcgaa
     3421 ttcctgtttc atcaaaggta agagtcaacc cttcggtttc catcaaagcg gaatactgta
     3481 aagttaaaga ggcgcttggc tcagtaagga ttcgtacgaa atcttccacc gagagggctg
     3541 acaactcgac acgtatcggc agacgccctt gcaattcagc aatcaagtca gaaggtttag
     3601 ctacatgaaa agcacctgaa gcaatgaata aaatatgatc agactttacc atgccgtatt
     3661 ttgttgaaac ggtagttcct tcaactaatg gcaataaatc tcgctgaact ccctcacgtg
     3721 atacatctcc cccacttaca gtatcagagc gtttggcaat tttatccaac tcatcaatga
     3781 acactattcc attttgctca acactttcaa tcgcttttaa cttgatatct tcttcgttaa
     3841 ttaattttgc cgcttcttcc tctctcaaaa tcttcattgc cttggcaacg gtcatttttc
     3901 gagttttcgt tctgtagctt ccaacttgtt gaaacataga ctgcaattgg ctagtcatct
     3961 cttccatacc tggaggagcc ataatttcaa tgccaacagg cgttgccgct acttcaattt
     4021 caatttcatt atcattaagc tcaccttcgc gcaattgctt acgaaatact tgacgagctg
     4081 tcgtgttttt ctcaccagga gttaatgttc ctcgagcagg agggagtaaa acatccagga
     4141 ttcgttcttc agccgcatct tctgccaggt gctccacttt tttcatggca aattcacgct
     4201 cttgcttgat tgccatatct gttaagtcac gaatgatcga atcaacatca cgccccacat
     4261 atcccacttc cgtgaatttg gttgcttcta ctttaataaa cggagcttta gccaaattag
     4321 ccaaacgtct tgcaatctcc gtcttaccaa caccggtagg gcctatcatt aaaatatttt
     4381 taggcattat ctcgttgcgc aatactggat ctttgatttt cattcgacgc cagcgatttc
     4441 gtaatgcaat ggctacggca cgttttgcat catcttgacc aataatgtgc ttatctaatt
     4501 cttgaactat ctctcgaggg gtcatgacca tattatttgc cttcatcatt taattcttct
     4561 atggttaaat tattattagt ataaatacaa atgtcgccag cgattgttaa agctttctgg
     4621 acaatctcct tggcagataa ctgggtgttt tccataagtg ctctggctgc agcttgagca
     4681 aaggggccgc cagaaccgat tgcaattaaa ctttcttcgg gctcaatgac atcgccatta
     4741 ccggtaataa ttaaagaagc cttgctatcc gctactgcaa gcaccgcctc taatcgacga
     4801 agaattctat cggttcgcca atctttagcc aattcaacag ctgctctaat caaatgcccc
     4861 tgatgcattt ccagttttgc ttcaaaccgt tcgaaaagag taaaagcatc tgctgttcca
     4921 cctgcaaaac cagcaatcac tttatcttta tacaatctcc gaactttacg tgcattgcct
     4981 ttcatcacgg tattgcctag ggtaacttgc ccatcaccgc caatgacaac ctgattaccg
     5041 cgtcgtactg aaagtattgt tgtcccatga aattgttcca aaataaaatc ctcaattaca
     5101 ttgcagaaat gtctaaatgg ggatcattgg taaaaaatca atagggattg agagaaaaat
     5161 taagtgttct taatatctga aaatactaat cgtcatatcc actctttacg gtcgtggctc
     5221 ggataatcgt tcaaaccgag ccacgaccgt aaggagtgga tataaaatta taaaaaagaa
     5281 acaccatgag tgagtggcga ggataattgc aaatgctccg caatactctg accaatatct
     5341 gcgaaacaat ctctccgtcc aataaatttg ctatttatct gagggccaaa cactaatact
     5401 ggaatatgct cgcgggtatg atcagatcca ggtaatgtag gatcacatcc gtgatctgct
     5461 gcgataaaaa ccatgtcatt gggttgtaag agaacctcca actctggcag acgcgcatcg
     5521 aattgctcta gcgcatgcgc ataaccagct atatcacgcc tatgcccata agaagaatca
     5581 aaatcaacaa aattggtaaa taccaagcta ccttgcgggg ctgttttcat tgcggataaa
     5641 gtggcatcaa acaaagccat attgccatca gctttgattt cttgcgtaac cccttgatga
     5701 gcatagatgt ctgcaatctt acctatagca atcacttccc gaccatcttc ctttagaaaa
     5761 tcaagcaatg ttttttccgg aggaggtgta gcgtaatctt ttctattacc tgttctctta
     5821 aatgagcctg gcttacctgt aaaaggacga gcaatcacgc gaccaatttg atatttatct
     5881 actaaatttc gagcaatttt gcagatatca tagagacgtt gtaaaccaaa tgcctcttca
     5941 tgtgcggcaa tttgaaatac gctatcggca gaagtgtata cgatcggctt accggtacga
     6001 atatgctcct cacccaactc gtccataatg atggttcccg aagcatgctt ttctcccaaa
     6061 actcctggca aattacactg cttaatgaat tctgaaatta attcttcagg gaaacagtaa
     6121 ggtttatcag ggaaatatcc ccactcgaaa gtcacgggca ccccagccat ttcccaatga
     6181 ccactgggag tatctttgcc caagctttgt tccactgcat atccataata tccagaagga
     6241 atagctaaag tagccaaatc gataaagggt aacccggaac tggccatagc agcatggtaa
     6301 agccccttgc ccgcaagatt tggaagcatt aaaggacctt tacgaacccc ttctatatcg
     6361 cactcacctc gtttacacgc ttcataaatg tgaaccaagg tgttagcacc cgcatctcca
     6421 taccgagctg catcaaggct ggctccgatg ccaaaggaat ccattactaa tacacaaact
     6481 cgtccggtca tactgtatgc tcaacttgtt ggcaacgagt ttcttttaat ccaaacaaca
     6541 aaagtaatgc gatcaatagt cctatgggta aaatgattgc tgcatgctga tacgcttcga
     6601 gtaaataaac tggcacacca ttaatcaaac gaacatcgcc ataattcatc aataaagcac
     6661 taaacaggtt ttgataaata agatacccgc cttgagttaa tatagacacg acactgacag
     6721 ctgttgcagt catcatggga gggctgcttt cagcaaccaa ggcatagcta atgacttgag
     6781 ctgaagtaaa taatcctaat aaaaagaaca atacggccat cattgtcact gaaacaggta
     6841 aataaagtac tcccagcaaa ataagtaaag aggcaaggac tccccctttc ataggcagca
     6901 cacgcaaccc taccttatca gatatccacc ccatcaaagg tgaacctata atggctccaa
     6961 aaaacaacat accgttgatc atagctgcct gatcagggct aatgcctaat ctctgttcaa
     7021 gatataaagt ccccatcatg gcgccaaata cagcaatggc catgttcata agactagtgt
     7081 ataaggctgc tcgcaaattt tgagcattta agtaggcttt tcttgcagcc aaaagcacat
     7141 tgataggttt tccttctttt ttgattgcga cttgaggtct ttctttaatc caaaaaaaca
     7201 tgagaactaa catggccaag cccaatatcc ctacatcttg aactgcttgg cgccatccaa
     7261 catatagaac cagtttagtt aaggggtatt gcgccagcat tcctcctgtc attgcaacag
     7321 ttacaatcgc gccagtcact aaagccatgc gctggggagg aaaccaatga gacgccaaac
     7381 gcacgggacc caaaaagcaa aatgcactac ctattccggt cacaaacctg caaaataaag
     7441 ccacataaaa cgaatctgag taggagagga taaacgtact aatcacacac agaaacatag
     7501 ctattaaaat ggtgtttttg attgaaaacc tgtccaaaac gattccggca ataaacagaa
     7561 aaatcacatt tgccaagtaa tagatactgg ataagtaagc cattttatct gcttgaatct
     7621 gaaaatcctg catgatattt gctgcaatgg aagcaaacat atttccctga ataaattcat
     7681 aaaagaaaaa catggttgct gcaaaacaaa ccagccatgg cattagcgat gctttaacat
     7741 cgaatgcttt aaattcatcc actacctgca ttatttctcc tctctatcgc ttgcagtatg
     7801 tttcacgagt catgagcaca gcaaccagtc ctgcaattgc ggcaacagga aacatccaca
     7861 tggcaaattg aaaatcacta accgtatatg ccgtaacatt tgttcccgcg tgatgtgcca
     7921 taagccaacc aaacagaact tgagcaacac cgcccccacc cataataatg acagaagcaa
     7981 ttccagttgc agcaccagta ttttctggct gattgctttc agcaaccagg gggtaagaaa
     8041 tcacttgcgt gctggtaaat aatcccaaag caaaaaagag gatactgaga ctcatttgag
     8101 tcagaaccac attgataaat agaggaatag tagtaatcaa agtagctatc gctccgaaaa
     8161 tcattaaggg tttgcgacgt ccctgagtat cggataacca accaaccaat gggcaaccaa
     8221 caacactccc catgaaaata aggcttacaa cattgcttgc cgcaatatca ggcaaatgat
     8281 gagctacctg caggtaactt gcaccccata gggcacacaa aaccataatg ggtaaattaa
     8341 gcagagaggt atacaagcct gccagccaat tttgtttatt agatagggct ttcatgaaac
     8401 tcgttagtat ttgtccttca ttggttaatt tatgtgctgg tgacttttca ggcctgtctt
     8461 gcacaatcat gtaaatccat aatattaaaa aagctccaac aaccccatca attaacaatg
     8521 cacgtcgcca accaaacaga tcattcaaat aagcaaaagg ggtatgcgcc atcatgccac
     8581 caataaatgc catggtaact aatgagccaa tgactaatgc ctgtcttctg ggaggaaacc
     8641 aatgagagac caaaacaaca caagataaaa agcaaaacgc attgcctatt cctgagagaa
     8701 aatggaaaaa tgaggcgaga aaaaatgatt ctgtaactgc aaaccctatt gttccaacaa
     8761 cacaaacaaa cattgcggtg agaataactt tcctggttga aaacctgtcg agaataagcc
     8821 cagctggtaa aagaaaaaga atatcagccc ataaataagt gctcgacatc caacttaatt
     8881 gggtggcatc tatatgaaaa tcttctcgaa gcgactgatt aataacatca aaaatattga
     8941 gttgaaaaaa ctcgtataaa aagaaaagcc ccgcggaaag acacactacc caagccataa
     9001 agtcaccacg gggaacaaaa gttttcgact ccactgcgac agaatgcgac accacctctc
     9061 ctttatttaa ggcctaaaaa ctgcggcaaa ttatatcaaa atttaaaaag tattggtatc
     9121 tttattgaac aatttatttc ttggaaaggt cttccctgct tcacctcgtt tataatgcaa
     9181 ccgacaggta gcaacatagg attcattgcc gcctatcaat acctgctcac cctcaacaac
     9241 agcttgccca ttttcatcaa ttctcatatt cataattgct ttacggccac aatgacaaat
     9301 tgtctttaat tcgattaact catctgccca tgccagtaaa aattgacttc cgggaaataa
     9361 ttcgcctcta aagtcagtgc gcaacccata agctagtact gggatagaca tttgatcggt
     9421 tatttccgtt aattgataca cttgctctcg agttaaaaat tgagcctcgt caatcaggac
     9481 acaagagtat tttgtggctt gtagttgcaa ttcttgagtc aaaacataga gattatccgt
     9541 gttgttaaac gcataagcct gctcagataa accgattcgc gaacagatag ttccatattg
     9601 gtaacgtgta tcgatagctg gggtaaacaa cagagtttgc atacctcgct ctctgtaatt
     9661 atagctagac tgcagtaaca ccgtactttt acctgcattc atagcggcat agtagaaata
     9721 taattttgcc atgtatttat tattttgagc ttcgtttgct ttactttact atgagatgga
     9781 attagaaacc actgctcaac aactcatcac aggtgaataa aacaatatca aggtatcccg
     9841 atgaactctg aatttgttga tgggtgtcaa ttttgcaagt aactctccgg gaatctgctg
     9901 aatttctttg ctattgataa ccaaatagtt ttccaaatgg ttagagaata atttacataa
     9961 ttcacttgga tttttaaccg atttcacttg accacccgag taaaactcag cagagaaatc
    10021 cgtcttgtat ccccaataaa caaggtgact tcctgctgtt gggttctgat tcagccatgc
    10081 agtaataatc ggcttatgcg tattaggcac aacgtctggt tttacaccaa gtacaaaaac
    10141 cactgccaaa aataaaactc cacagagtaa tgaggagata aggacccact tcaaatgttt
    10201 caattccaaa ttcaagcgat tcaaaatttc agcaaaaagc aaagcaaatg cgggtagaga
    10261 aggaaacaca taggggtata tgatattgct ggcaaaagtg aaaaaaagaa gcggagtaat
    10321 catccaaaga gttaaataac ttaaccatcc atcttcactt tggcacagag agggcaacat
    10381 ttttccatgt ttcaccagcc agccgcctgc tacaatattc caaggaaaga tgcctgctat
    10441 agcatataac catattgttc ctttaggcac atggtgcact atcccgtatt tatcaccaga
    10501 ccagccaggg gttaaaaatc gatggaaatg ctctcccagg ataaaataat ttaaaaatcc
    10561 tggggttcgt aattcagcca gaacatacca gggcatagca attaacaaaa caagaaatgt
    10621 gcccttaaac cacggtaatc tcttccatag attaagccat tcattgcgta gcagaaccca
    10681 gaaaaataca accattccga ccagtaccac agcaattggg ccttttgcta ataaacccag
    10741 gcccaatcct gcgaaaaatg cataagacca tatcttgttt tcataaacca tggcatgcca
    10801 aaacgagaca cttgcaagcg tgatacaaaa aagtagggca gggtcagtca tcacggtccc
    10861 tgcatcaagg aaaaaataaa gcgaccctgc cagtgttaat agggaaaaga gcgcagccat
    10921 tgagccactc tgtttttttg ccactcccca aaccaaccat aaaacaccaa cagagagcaa
    10981 aagacctggc aagcggactg cgaattcatt aacacccaat agttgcataa aaaaagcaga
    11041 caaccagatt gataaaggtg gtttagccca aaatggaaca ccataatcct gctgcaaagt
    11101 cacccagttc cctgtttcca gcattttgcg tgcaatttca gcgtagcggg attcggtcgt
    11161 atcaaacaaa ggaaaaaaat aattggcaat caagcggcat atcagcagaa caaccaacac
    11221 aaacaaagta taactaaaga atttttgctg aatctcttta tctgtttgaa aactcaaaat
    11281 atacctcttt tatttaaaca actaaatcgg agtaccattt agtaaagcat tggagtgagc
    11341 ctgtggtatc tcggacatag cgttcccaga aataaattcc ataaagccaa ttattcccta
    11401 acctcccttt tccttttgat agatctttac acccaagcca ggtttcttaa tttttaaatg
    11461 attattatat atttttaaat tgtataatga aaatacatga cgtcagctta agtttagttt
    11521 acactggcca ctgtaagtat ttcttacatt aatcttactt ggatttttgg agactaacat
    11581 gacgtttgtt ttaaaggaat ttgatgtact aaaaagtcat tttaatgaca ccgttaaaat
    11641 catccttcaa cgcgaaaaaa aagacaaaat tgaagacctt cccaacccca gaaaagaaga
    11701 acttcaattt ctgaccgctg ttctcaatca attggaagca aaaatagatg aactgaaacc
    11761 ccgttcattg gcaagctatg tccatgtttt ttatggcgct atgctgctgg tttgcaaaga
    11821 tgttgaaaat aatctgcggg tgatggaaaa gaaagaaaac agcttattgt ttactcgctt
    11881 gatggatgga atgggtatta cagatgaaaa catgccgacc tctgagcaaa acatcatgtt
    11941 ttacagagga ttaaacaaat tcttaaattt catttatgaa agcaatgatt ctcgtaaagg
    12001 cttaaaaaag gagcatttcc tgcaagtcct ttcgttaaaa aagatatact ctttagccaa
    12061 attaagttat gagcaggaag aagcagctga aaataatgct ttgtcaaagt taactgctga
    12121 tggaaaaacc aaagccaatg cgaacagctt ccatgtggaa aaaccaatcg attcatccat
    12181 tgttgagcaa ttcaaatcct gggatgaaat gaaaggcgct cttcatcaat taattctcga
    12241 tgaactttct gataagaacg ttgctaaaat ttcagcttta agtcaagccc gctcagcaca
    12301 actgaaattt ttacaaacga tggcagaaca gctagacaag atccctaacc aatctcttga
    12361 accgtctgag aaaatggcca ttcttgctgg agcaatgtat atcgttcgtg gccaaatagc
    12421 ccaggagtat ggaaaagatc cattaagcaa cgataaaatc agcgctactg tgattcatac
    12481 tggtttaagc acgatacttc atgctaatgc cgattgctgt gaagacaagg aagtactgat
    12541 tgctgctgcc aataagttca ttcgtcatat ggtcattgaa cgtcctgaac agtcaaataa
    12601 aaaaattact aaagaatcca ttcgagaaaa caacatgttt tccgacatag ccggattcca
    12661 attagtttct gtcttgacgc taatacaaaa catgatcaaa acatgtcgta ctgatgccat
    12721 tgaagcttgt gtcaccaagc gtaaggaaga actcgaagca ttaaaaccca aaaaagaggg
    12781 ttattccatt gcgagttcag tcactggata tgtaggtagc tggtttaaaa aagcaccaag
    12841 catatctgaa gaagacgaag aagatgactt aaaagatcaa agcacagcag aagagaccag
    12901 taaaccgact gtataattaa tttgaacaat taacgtcgat tttcattcat gaacagtctt
    12961 attttcaaaa taagactgtt tatttcgtat ttctt
//
LOCUS       DBNCWK010000088        12991 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.088, whole genome shotgun sequence.
ACCESSION   DBNCWK010000088 DBNCWK010000000
VERSION     DBNCWK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12991
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.088"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..2296
                     /locus_tag="AC06IO_002055"
     CDS             <1..2296
                     /locus_tag="AC06IO_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional SulP family inorganic anion
                     transporter/carbonic anhydrase"
                     /protein_id="HIO9875231.1"
                     /translation="NIVNLRLFRIYSKRYLKFDFVAAIVVFLVAIPLCLGIALASGAP
                     LFSGILSGIIGGIVVGIFSGSQVSVSGPAAGMAAVVLAAISQLGDFNTFLLALTIAGL
                     LQMIIGALRAGFVADYVPSNVVQGLLCSIGILLIIKQLPLAFTLSSDFDELKTHLLET
                     TEGFTVSPLLALSQHINEGALIITTLSLAILIYFDITKNKILKEIPAPILVVLAGILL
                     NELFIWTNSSLAQNSPQLVNIPDTNGFFQFFSHLEYPDWSAWTNPKVYLYALVICIVA
                     SLETLLNLKASERLDKKRRHSPTNQELVAQGLGNITSGLVGGIPVTSVIVRTSINIHA
                     GSKTKFSAVLHGFFILFAVMLIPGALNKIPLSSLAAILIYTGYKLNKPAIYINIFSQG
                     SDRFIPFIVTVISIIAFNLLAGILIGLAISLFYILKSNSQARIDIIKEIYPNGSTYRL
                     MLPQQMTFLNKAALVAELDTLPRRSQLIIDARHSQYIDKEILDLFNELKEELAGSKQI
                     SVNFTGFQEHYKIHNYIDFITVTTYDVQTNLTPAQVLNILLEGNQRFLSDNRIHRSNQ
                     IDIKYTAKTQHPIAVVLACIDSRVPVETIFDMSFGDLFCVRIAGNVINDDILASIEYA
                     CNVVGAKLIMVLGHTRCGAIQSACDGIEKGHITQLLSKIKPAVNAEKETTTERNGKNQ
                     TFVNHVTELNVANTLQNIYKKSDILRTMIDSNEIGMVGAIYDVSSGKVSCKTYTEELS
                     TLDGDENKLLAQKFETILLEAKAS"
     gene            2383..4095
                     /gene="lem3"
                     /locus_tag="AC06IO_002056"
     CDS             2383..4095
                     /gene="lem3"
                     /locus_tag="AC06IO_002056"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector phosphocholine hydrolase Lem3"
                     /protein_id="HIO9875232.1"
                     /translation="MKLRYIINENKLVFTSCNMRDKIITGKKIIFSQSVAKDQTKNIS
                     SFLSERFYSVNQSHNHSIIIGSSLSHQENDIEHDTILDTSGVLVTTDTNGIVNGARVA
                     ITDGLGGGNGDQEEDDEIYRVSHSSCANFLNCDQNIDTTLSLITQPKASDKKQTAPKT
                     LQHTEASMAAFIYQNHQGKGYIGEFANIGDGLIIILDKRFKIKHMVSACHIYRGFGTW
                     TPPSLQALATTANKDALLVRQTLKLAEGDIIISMTDGVWGELKTSLIAQTNDRRDIGV
                     DKEYFKTLFDELTDAPYPSSFDIARIITHRAMSRSLERRKTLIKLINEIEQQHFHEKS
                     VKTINEVLEYFIKTGHVETAQTLKAILFEDGLSDGITYFENIEIPLEMVMHDLKSRTV
                     GDCSTINVTRIPYHLDELIRGFINYPEKHQILAPLFKARVKSEADLEEAFHRLSLEMV
                     QPEIECPISEAHFEPAFKKETLDKTQAVLTHYFRISTGLDSKKNYQERLNDLSAYFSK
                     ESSLEKNDIKLLLSMLDSEIKPKTGVFQTLFGENQNKLYKAFHKKIELQLLDGEIENK
                     NELK"
     gene            complement(4102..6951)
                     /gene="ankN"
                     /locus_tag="AC06IO_002057"
     CDS             complement(4102..6951)
                     /gene="ankN"
                     /locus_tag="AC06IO_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkX"
                     /protein_id="HIO9875233.1"
                     /translation="MVKIMPNLPGLYFLQAYPREEIWRLFVDGRFWSKENGWRGYESR
                     EPGCLNAALESLCSIALQVEKSGEEFELSVDLIKKIHKKCGKKVEELQEKNPGELRTD
                     EPVSFGIPAGRASIKGIEEFLSLVFLTEGEAEFGPGKAGPFGPNFNKNYFKNLNPEQI
                     PDLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEKITQSYNQEIKTAETLDEKLKII
                     VKHIRMYEVLHPFRDANGRTFVNNLLNILLMQQGLPPATFYEPNVFDLYSAEELVVVV
                     KEAIFNTVEIIEQSKKKTPITLYGYHSSLEEQTKFRDMLDSPSYEKIKHMDFSDLNPE
                     KLHRKTQKCLSSLNEQYPLHRGAIYLSDPGEIKLLLSNRNESQINQQIEQGAPPIYVG
                     KTPAHLAVISGNMAMLDELIAKKADLSLQDYDGKTALHYAAECGNMQIMGKILKVVLS
                     QEDAIKVLNIKDNHGKTAFHYAAEFGTPELISALTTTEVIQINEPDNSGSSAITLAYK
                     NHKLKIFDELLNSGADISDELLDAIWARKDKETLGKIIAKNEKILLNKEAFRIAISLG
                     SVSLVKKFLRAGVDIDIPLTKDKATPLMLSINSGNPKLVSYLLKKGANTRLTDTSGNS
                     VLHYVFYSKAENREALANIITEKDKKLINQPNANGNPPLYNAVVVNDLKMATILLEMG
                     ARVDFEDRLGNNILHSAMRRCDLPIILDIVKKDSTLLHKRNSERRNPFHQALHEMHTF
                     PSSKETEEIHFMNLSDFLLKEGVDLNKKDIKGKTILDIALSKQYFHLCVKLMKAGAHT
                     NISSSSKFLKNSDANSILERPFKFKNDLKKELDDNPLIAMAQINDLYVQIKNNRIRTP
                     TGYAPKEGVSFFKGKSNDAKAHDEVLSVLKELYDSKLTEMLGNLPGEGLEEIKRSQKF
                     FDGELKLLIKNQDISRKVDKKSIQEAVGTSLKLKW"
     gene            7113..8822
                     /locus_tag="AC06IO_002058"
     CDS             7113..8822
                     /locus_tag="AC06IO_002058"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIO9875234.1"
                     /translation="MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMP
                     LGLRVLRKVENIVREEMNKTHAMELLMPSVQPAELWQETGRWETFGGQLLTMKDSNQR
                     EYCFGPTHEEVITDIMRNELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHLSLESLQETYKDMYQAYCRIFDRMGLKYRAVEADTGAIGGSTSHEFQVLAESGE
                     DLIFYSDASDYAANIEQATSLKPPKANQSCNETITLVDTPNKKTIDEVASFLGIASNQ
                     TIKTLIVKGKEHPMVALVLRGDDELNEVKATKHPLVHSPLSFIDEELILKTLKTPLGS
                     IGPVKLNIPVIVDHQALAMPSFVCGANQADKHFINAAWERDAKYDDAYDLRNVKEGDP
                     SPDGRGTLHCCRGIEVGHVFQLGDKYAKAMNASVINEQGQLQTMIMGCYGLGITRVVA
                     AAIEQHHDEHGIIWPQALAPFQVNIIPLNGARSQAVKEQAESLYQQLKSHGIDVLLDD
                     RNERAGVLFADNDLIGIPHRLVVSERNLEQGCIEYKSRTSSETQLINLDKVVNFIIEL
                     INK"
     gene            complement(9180..>12991)
                     /locus_tag="AC06IO_002059"
     CDS             complement(9180..>12991)
                     /locus_tag="AC06IO_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875235.1"
                     /translation="NVQSLLEIHSVLLKDISSLFDYPPYPALDVFSEKLREYSRELKL
                     YIDSFDRDPAGARAPKVNQEGILVKSRERILNEQFDTSQIQEVIAKIESLADGASLSC
                     KRQYELAQQVVFINAIGRNFPLKIASATYTDLTQLGRNNLQELSDFLIMEMRRLDISK
                     EDKLRTQLKLLAVMREQYFRSTGIFFDTTQMIFLLLSLHHQEQNVLMEVRRNENLSAP
                     PAAILAAMQWVLAEGGTVDVCFANHGLIAQDYLEKGDRDFFTCLGIPSSVVEANSEFG
                     TYQVGGINYSTLSDLTSYRSHAEIEDEELSMDRYGYPVSSYLILNHIDFSNVDDRTLS
                     HFSSLYDYYKGKGRVVRITEIEETVGEVVGQISRFDMDVGYRVFPARKNLQEELNTQS
                     LAERRREEAVKQYFIQSVSNIQQAVLNQLEEWQAFLHLIYPKSEWRQLDNELFVQRNE
                     LIDSLKKQWTKCLEDSDPKKMYPNPYVRRDVFQRLQTRELEKAIQAYEKSVSSIWSLN
                     RDQLKAKTENRFSSDSVNALRCKYLEEVDLSEQIKWSKLSARLKKKEATQEKKRSHRH
                     LESAVEVTGAMLSYHDGDLEPYRLAFVNSQLKLWVRDISKEINNSSLPARVKQGLIER
                     ADNADNFLALELLLIDYESRWLDKEKLSEKFRMQPIISDMLRVYQQAGFEEHKELKIL
                     KETYLDNTATQIIHHLDRALAWAKPENRGPWYWIERSAVKKAANEILVLANGVKQARD
                     SLSKQVAIKKLYRALCKHQAQLEDVWIFSFGHKNTRDLINQTLNTMDSLTVLGRDEGG
                     LDINFIQECKEEAQYYLLQEKFNSILEEFEKNNSQILTAHSEWQEVKRQLQQKQNESS
                     KVYTLNEMYYVLCKKINELSISYSLLREPLVQLRGMIRTLWNSFQEKHQGIMDESKYF
                     DFKARKIKEELEGIEGYSVFNVKVKKGYTGFNEYVELIIEGEGTSALFEGFMQYKSRL
                     NELKEAYRQLEFSLKQNRLELLNLNRIHAKHLPFLKDSIKGNPEVELFPEQCQSKVKD
                     ILTLKEWINGQIPENLSQFPENMQNHFLDRNTLKSMDRNTMTMEQIDKIKDEQLKADL
                     TELYNKMHKIDEEQSKSWFSTVTRGIVGLIPVMFRQEETEDDWRYQFNELQGRSDSIL
                     QSLLTKKIMTKYDALVNEMVLIQSNLAQRMDSGNKKIQFLSDKILEEERKTNVIIKRF
                     DNLNDFYDFEAKLKRFKTSPPIAPVNEVQIESKNRILSYGEGAFSNPEEEFQTDSVMQ
                     PPMVFNI"
BASE COUNT         3946 a         2600 c         2277 g         4168 t
ORIGIN      
        1 aaatatagtt aacctgcgtc tatttcgtat ctactctaaa cgttatttaa aatttgattt
       61 tgttgctgca attgtcgttt tcctcgtggc gattccttta tgcttaggta ttgccctggc
      121 ttctggcgca cctctttttt ctggtatctt aagtggaatc attggcggta ttgttgtggg
      181 tatatttagc ggctcacaag ttagtgtcag cgggcctgct gcgggtatgg cagccgttgt
      241 attggcagct atctctcaac ttggtgattt taatactttc ttattggctc ttacgattgc
      301 aggcctttta caaatgataa taggtgcatt aagggcagga tttgttgcag attatgttcc
      361 ttcgaatgta gttcaaggct tactgtgttc aatcgggatc ttgctaatca ttaagcaatt
      421 accacttgca ttcactctct catcagattt tgatgagctt aaaacacatt tattggaaac
      481 aacagaaggg tttacagtaa gtcctttgtt agctttgtcg cagcacatta atgaaggcgc
      541 actcatcatt actactcttt cattagctat tttaatctat tttgatataa ccaaaaataa
      601 aattctgaag gaaatcccag cccctatcct tgtagttctg gcaggaatac tgttaaatga
      661 gcttttcatc tggacaaact ccagcctggc acaaaactcg cctcaactgg ttaatatccc
      721 agataccaat ggttttttcc aatttttcag ccatcttgaa taccctgact ggtctgcctg
      781 gactaatcct aaagtctatc tctatgcgct agtcatatgt atcgttgctt ctttggaaac
      841 gttgcttaat ctcaaagcgt cagaacgatt ggataaaaaa agaaggcaca gcccaactaa
      901 ccaggaatta gttgctcaag gcttgggtaa tataacatcc ggtttggttg ggggtattcc
      961 tgttacatca gtcattgtca gaacatccat taatatccat gctggttcaa aaaccaaatt
     1021 ttcagccgta ctccatggtt ttttcatctt gtttgctgtc atgttaatac ctggtgcatt
     1081 gaataaaatt cctttgtctt ctctcgctgc aattttaatt tacactggct ataaactaaa
     1141 taaaccagcc atctatatca atatattttc acaaggcagc gatcgattca tcccttttat
     1201 agtgaccgtg atcagtatta ttgccttcaa tcttcttgca ggtatattaa taggtctggc
     1261 tatcagtctc ttttatatat taaagtccaa tagccaagct cgtattgata tcattaagga
     1321 aatttatcca aatggttcaa cataccgttt gatgcttccc caacaaatga cttttcttaa
     1381 caaagctgcg ttggtcgctg aattggatac attgcccaga cgttctcaac tgattatcga
     1441 tgcacgtcat tctcaataca ttgataaaga aattctggac ttatttaatg aattgaaaga
     1501 agagttggca ggcagtaaac aaatttctgt aaatttcacc ggatttcagg aacactataa
     1561 aattcataac tacatcgatt tcattacggt tacaacctat gatgttcaaa ctaacctgac
     1621 gcccgcacaa gtattaaaca tactgcttga gggaaatcag cgctttttaa gtgataaccg
     1681 gattcatcga tcaaaccaaa tcgatataaa atacactgca aaaacacaac atccaattgc
     1741 tgtggtttta gcctgtatcg attcaagagt tcctgtagaa accatctttg atatgagttt
     1801 cggtgatctt ttttgtgtcc gcatagcagg caatgtgatt aatgacgata ttttggccag
     1861 catagaatac gcctgtaacg tagttggtgc aaagcttatc atggttctgg gacatacccg
     1921 gtgcggggcc attcaatctg cctgtgatgg tattgaaaaa ggtcatatca cccaactgct
     1981 ctctaaaatt aagcctgcag ttaacgcaga aaaagaaact acaacagaac gtaacggaaa
     2041 aaatcaaact tttgtaaatc atgtcactga attaaatgtt gcgaatacct tacagaacat
     2101 ttataaaaaa agtgatattt tacggactat gatagacagt aatgagatag ggatggttgg
     2161 tgctatttat gatgtaagca gcggaaaagt gagctgtaaa acatataccg aagagttatc
     2221 cactttggat ggggacgaaa ataagctcct ggctcaaaaa tttgaaacca ttcttttaga
     2281 agccaaagca agttaaactt ttttgaaatt gtaaaaacac cggaaagaaa taaatttctc
     2341 aagccaggca taagattttc tacaagtcat taagcctggc gtatgaagtt acgctatatt
     2401 attaatgaga ataagcttgt ttttacgagt tgcaatatga gagataaaat tatcaccggg
     2461 aaaaaaatta ttttttcaca atccgttgca aaagaccaaa caaaaaatat atcaagtttt
     2521 ttatcagaac ggttttactc tgtaaatcaa agccataatc attccattat tatcggttcc
     2581 agtctttctc accaagagaa tgatatcgaa catgatacca tactcgatac atcaggcgtg
     2641 ttagtgacta ctgatacaaa tggcatcgtt aacggggcaa gagtcgcaat tactgacggt
     2701 ctgggtggtg gaaatggtga tcaggaagaa gacgacgaga tatacagagt atctcattcc
     2761 agttgcgcga attttttgaa ttgcgaccaa aatatagata caactctaag cttgattacc
     2821 caaccaaagg cctctgataa aaaacaaaca gcaccgaaaa cattgcagca tacagaagcg
     2881 tctatggctg cttttattta tcaaaatcat caggggaaag gatacatagg tgaatttgca
     2941 aatattggtg atggactaat tattatcctg gataaacgtt ttaagataaa gcatatggta
     3001 tccgcatgcc atatatatcg cggatttggt acctggactc caccatccct gcaagccctg
     3061 gcaacaactg caaataaaga cgccctgctt gtcagacaaa cacttaaact tgcagaaggc
     3121 gacattataa tatcaatgac tgatggggtt tggggggagc tgaaaaccag tttgattgct
     3181 caaaccaatg accgtcgaga tataggagta gataaagaat atttcaaaac gctatttgac
     3241 gaattgacag atgctcccta cccttcttca tttgatatcg ctcgtatcat tacccatcgc
     3301 gccatgtctc gcagtttgga gcgtagaaaa acgttaataa aattaattaa tgaaatagaa
     3361 caacaacact tccatgaaaa atctgtcaaa accattaatg aagtcttgga atattttatt
     3421 aagacaggcc atgtggaaac agcacagaca ttaaaagcaa ttctctttga agacggtttg
     3481 agtgatggaa taacttattt tgaaaatatt gaaatccctc tggagatggt aatgcacgat
     3541 ttaaaaagtc gtaccgtcgg tgattgttca accatcaatg taacgcgcat tccttatcat
     3601 ttagatgagc tcatacgagg ttttatcaat taccctgaga aacatcaaat cctggcgcca
     3661 ctatttaaag caagggtaaa gtctgaggct gatttagaag aggcatttca tcgtctgtca
     3721 cttgaaatgg tacaacctga aatcgagtgc ccaatttctg aagctcattt tgaacctgct
     3781 tttaaaaaag aaacccttga taaaactcag gctgtcttaa ctcactattt tcgtatttcg
     3841 actggtttag atagtaaaaa aaattatcag gaacgtttaa atgatctgag tgcttacttc
     3901 tccaaggaat cctctctcga aaaaaatgat attaaattgc tattatctat gctagatagt
     3961 gaaataaaac ctaaaaccgg ggttttccaa acattgtttg gggaaaacca aaataaatta
     4021 tacaaggcat ttcacaaaaa aatcgagttg caattattgg atggtgaaat agaaaataaa
     4081 aatgaattga aatagcagtt cttaccattt taatttcaag gatgtaccaa ctgcttcctg
     4141 aatagacttt ttatcgactt tcctcgaaat gtcctggttt ttaattaata attttagttc
     4201 gccgtcaaaa aatttctggc ttcttttgat ttcttctaat ccttcgccag gaagattacc
     4261 gagcatctct gtgagtttac tatcatagag ttcctttaaa acagataaaa cctcatcatg
     4321 tgctttggca tcgtttgatt ttcctttaaa aaaggatact ccttctttcg gggcataacc
     4381 tgtaggagtc ctaatcctgt tatttttgat ttggacgtac aaatcattaa tttgtgccat
     4441 agcaattagc ggattatcat cgagctcttt ttttaaatcg ttcttaaatt taaaaggacg
     4501 ttccaaaata gaattggcat cagagttttt aagaaatttt gatgaagaag atatattggt
     4561 atgggcacct gctttcatca gtttgacaca taaatgaaaa tactgcttgg ataatgcgat
     4621 atctaatatt gtttttcctt taatatcttt tttatttaaa tcgactcctt cttttaaaag
     4681 gaaatcactt aaattcatga agtgtatttc ttcggtttct tttgaggatg gaaacgtatg
     4741 catctcatgc aatgcctgat gaaaaggatt tcttctttca gagtttcttt tatgaagtaa
     4801 ggtgctatct ttcttgacaa tatccaatat aatcggcaaa tcacatcttc tcatggcact
     4861 atgcaaaata ttatttccca gcctgtcttc gaaatcgacc cttgccccca tttctaataa
     4921 aattgttgcc attttcaaat cattaactac cacagcatta taaagaggtg gatttccatt
     4981 agcatttggc tgattaatta atttcttatc tttctcagtt ataatatttg ctaaagcttc
     5041 cctgttttct gcctttgaat aaaaaacata atgcagcaca gaattaccac tggtatcagt
     5101 taaacgtgta ttagctccct ttttcaataa ataactgacc aatttgggat taccggaatt
     5161 tatagataac attagcggtg ttgctttatc tttggttaag ggtatatcaa tatcgacccc
     5221 ggcacgtaaa aattttttaa ccagactgac acttcctaaa gaaatagcta tcctgaaggc
     5281 ttctttattc aaaagaattt tctcattttt ggcaattatt ttacctaatg tttctttatc
     5341 cttccgagcc catatcgcgt ccaaaagctc atcactgata tcagcaccgg aatttaatag
     5401 ttcatcgaaa attttcaatt tatgattttt atatgccaaa gtgattgctg aagaaccgct
     5461 gttatctggt tcattaatct gaataacctc tgtggttgtc aaggctgata ttaattccgg
     5521 ggttccaaac tcagcagcat aatggaatgc tgttttgcca tgattatcct tgatattcag
     5581 cactttgatg gcatcttcct ggcttagcac aacttttagt atttttccca taatttgcat
     5641 atttccacac tctgcagcat agtgaagagc agtttttcca tcataatcct gaagagataa
     5701 atccgctttt tttgcgataa gctcatcaag cattgccata tttcctgata tgacagccaa
     5761 atgagcagga gttttaccaa cataaatagg cggtgctcct tgctctatct gctgattaat
     5821 ctgagattca ttgcgatttg acaataataa ttttatctca ccgggatcac taagatagat
     5881 tgcgcctctg tgtaatggat attgctcatt tagtgatgat aaacattttt gagtcttccg
     5941 atgcaatttc tcagggttta aatctgaaaa atccatatgt ttgatttttt catacgatgg
     6001 actatccagc atatcccgaa atttggtttg ctcttccaag cttgaatgat atccatataa
     6061 agtaatggga gtcttttttt tactttgttc aataatttct accgtattaa aaatggcttc
     6121 cttcacaaca acaactaatt cctcagcgct atataaatca aagacattag gttcgtaaaa
     6181 cgtcgctggc ggtaaacctt gttgcattaa aagaatattt aataaattat tgacaaaagt
     6241 tcggccatta gcatctcgaa aagggtgtaa tacttcgtac attcgaatat gtttaacgat
     6301 aattttaagt ttttcatcaa gagtttcggc agtttttatt tcctggttgt aagattgggt
     6361 aattttttcc aggtaaacat ccacattttt cattactgca agataaaagt gattagtatt
     6421 actatgccca tatttgcaca tatcgaaata gatttgcttg gcaagatcag gtatttgttc
     6481 aggattcaga tttttaaaat agttcttatt aaagtttggg ccaaatgggc cggctttgcc
     6541 tgggccaaat tcagcttcgc cttctgtaag aaataccagg ctcaggaatt cttcaatacc
     6601 cttaatcgag gctcggccag ctggaatgcc aaatgaaaca ggttcatctg ttctgagttc
     6661 acctggattt ttttcctgta attcctccac cttttttcca cacttcttgt gtatcttctt
     6721 tattaaatca acgcttaatt caaattcctc tcctgatttc tcaacttgca atgcaataga
     6781 acataaactc tccagagcgg cgttaaggca tcctggctct cgcgactcat agcctctcca
     6841 cccattttct tttgaccaga agcgcccatc aacaaataat ctccatattt cttcgcgtgg
     6901 ataggcttga agaaaatata atccaggtag attcggcata atttttacca atttaaaata
     6961 ccaatttatt aataattata ggacaatgtg agcaaatata gtcttttgag aagaacagag
     7021 aaacgaagga cctaaactaa acatatcgca aattgaaggg tgataagtat ataatatcga
     7081 ccttaattat ttatagaaat ccaggaatag acatgcgcgc atctcaatgg tttttggtca
     7141 ctcaaaaaga aactcccaac gatgcagaaa tagcatccca tcaattaatg ctaagatcag
     7201 gaatgattcg taagcttggc tcaggacttt atacctggat gccacttggc cttagagtgc
     7261 ttcgtaaagt tgaaaatata gttcgggaag aaatgaacaa aactcatgcc atggaactgc
     7321 ttatgccatc agtacaacct gctgaattat ggcaagagac aggacgttgg gaaacctttg
     7381 gtggtcaatt gttaaccatg aaagacagca atcaaagaga gtactgcttt gggcctaccc
     7441 atgaagaagt cattaccgat atcatgcgta atgaattgca atcgtataaa caattgccag
     7501 tcaattttta tcaaatacaa accaagtttc gcgatgaaat cagaccacgg tttggggtaa
     7561 tgcgagcccg tgagtttatt atgaaagatg cttactcctt ccacctcagt cttgaaagcc
     7621 tgcaagaaac ctataaggat atgtatcaag cctattgcag gatttttgat agaatgggac
     7681 tcaaatatcg agcagttgaa gctgatacgg gagccattgg cggttcaacg tcccatgagt
     7741 ttcaggtttt ggctgaatcg ggtgaagatc tgatttttta tagtgacgcc agcgactatg
     7801 ctgcaaatat tgaacaagcg accagcctaa aaccacccaa agcgaatcaa tcatgcaatg
     7861 aaacaatcac attggtcgat actccaaata aaaaaacaat tgatgaagta gccagcttcc
     7921 ttggtattgc cagtaatcaa accataaaaa ccttgattgt caaaggcaag gaacatccta
     7981 tggtggctct ggtcttaaga ggagacgatg agctcaatga agtcaaagca accaaacatc
     8041 ccttagttca ctctcccctg tcctttattg atgaagagct gattttaaaa acgctaaaaa
     8101 ctcccttagg ctccatagga ccagtcaaat taaacattcc tgtcattgtt gatcatcaag
     8161 cgttggccat gccttcattt gtctgtggtg ccaatcaagc ggataaacat tttatcaatg
     8221 ctgcatggga gcgcgatgca aaatacgatg atgcctatga tttacgcaac gttaaagaag
     8281 gtgatccaag ccctgatgga agaggaacgc ttcactgctg tcgtggaata gaggtaggac
     8341 acgttttcca attgggtgat aaatacgcca aagctatgaa tgcgtctgtt atcaatgagc
     8401 aaggacaatt gcaaaccatg atcatgggct gctatgggtt agggatcact cgtgtagtcg
     8461 ctgccgcgat tgagcagcat catgacgaac acggaatcat ctggccccaa gctttagcac
     8521 ctttccaagt caatattatt cctcttaatg gcgcgagatc acaagccgtt aaggaacagg
     8581 cagagtctct ttatcagcaa ctcaaatctc atggcatcga tgtattactc gatgatcgta
     8641 atgaaagggc aggtgtatta tttgctgata atgacttgat agggattcct caccgattag
     8701 tggtcagtga gagaaatctg gaacaaggat gtattgagta caaatcccga acttcaagcg
     8761 aaactcaact gatcaatctg gataaggtgg ttaattttat tattgagtta attaataaat
     8821 aagattttct accccattga caccgcgtta tctgcattcc ggcaatgctg ttcggattaa
     8881 aaataacccg aacggcatta aagagaagct aataatacag gacttgtatt taaagaatca
     8941 caaataaagt tctttgccca ggttcaccat atctcgtttg gtactcatta atgtgagtta
     9001 tggataagac cagttgtcac ctcgaatcgt tcgggctgag gaggcattta tgccgtctcg
     9061 aagccttgta cgggatttaa tgttgtgcgc caaggcttcg agacgatgct tatgcatctc
     9121 ctcagcccga acgaaccttg gatatcaact ggttttataa tccttatcca tcactgacgt
     9181 taaatattaa aaaccattgg aggttgcatt acagaatccg tctgaaactc ctcttcagga
     9241 ttggaaaacg ccccctcacc ataagaaagt atcctgtttt tagattctat ctgaacctca
     9301 tttactgggg ctattggagg cgaggtttta aaacgtttta gtttagcctc aaaatcataa
     9361 aaatcattga ggttatcaaa acgcttgata atcacattgg ttttcctttc ttcttcaaga
     9421 atcttatcgc ttaaaaattg aattttttta ttaccagagt ccatccgttg tgccagattg
     9481 ctttgtatta aaaccatttc attaaccaac gcatcatatt ttgtcattat ttttttagtt
     9541 aacaacgatt gcaaaatgct atctgagcgt ccttgtagtt cattaaattg atacctccaa
     9601 tcatcctctg tctcttcctg tctgaacatg acgggaatca aaccaacaat cccgcgagtc
     9661 actgtagaaa accatgattt ggactgttct tcatcgatct tatgcatttt attatataat
     9721 tctgtaagat cagcttttaa ctgctcatcc tttatcttat cgatttgctc catggtcatc
     9781 gtatttctat ccatgctttt taacgtattt ctgtcaagaa aatgattttg catgttttca
     9841 ggaaattgac tgaggttttc aggtatttgg ccattaatcc actcctttag tgttaaaata
     9901 tccttcacct tgctttggca ttgctctgga aacaactcta cttcgggatt gcccttgatg
     9961 ctatctttca aaaaaggtaa gtgtttggca tggattctat tcagattcag caactctaat
    10021 ctattttgct taagagaaaa ctctaactga cggtacgcct cttttaattc atttaacctg
    10081 gatttatatt gcataaatcc ttcaaataat gcggatgtcc cttctccttc aataattaac
    10141 tcaacatact cattaaatcc tgtatatcct ttttttactt tcacgttaaa aacagaatat
    10201 ccttctattc cctccagctc ctccttaatt tttcgcgctt taaaatcaaa atatttcgat
    10261 tcatccatga tgccctggtg tttttcctga aaactattcc aaagagtacg aatcatccct
    10321 cgtaattgca ctaaaggctc acgcaatagg gaatatgaaa tggataattc attgattttt
    10381 ttacaaagaa cataatacat ttcattcaat gtataaacct tgctactctc attttgcttt
    10441 tgctgaagtt gccttttaac ctcttgccat tcagaatgag cagttaagat ctgactgtta
    10501 ttcttttcaa attcttctaa aatagaattg aatttctctt gtaacaaata gtattgagct
    10561 tcttctttgc actcctgaat aaaattaata tccagcccac cctcatctct acccagaaca
    10621 gttaaactgt ccatggtatt taatgtttga ttaatcaaat cacgtgtatt tttatgacca
    10681 aatgaaaaaa tccaaacatc ctccaattga gcttgatgtt tgcataaggc tctatataat
    10741 tttttgatag caacctgttt ggataaagaa tctcttgctt gttttactcc gttggccaaa
    10801 accaatattt catttgccgc tttttttact gctgaccgct caatccaata ccaaggtcct
    10861 ctgttttcag gcttagccca agccaatgcc ctgtcaagat gatgaatgat ttgtgtagct
    10921 gtattatcca gataggtttc ttttaaaatc ttaagttctt tatgttcttc aaagcctgct
    10981 tgctgataca ctctcagcat atcactgata attggttgca tgcggaattt ttcgctcaat
    11041 ttctctttgt ctaaccatct gctttcataa tcaattaata acaactccag agcaagaaag
    11101 ttatccgcat tatcagctct ctctattaga ccttgtttaa cgcgggcagg taatgaagag
    11161 ttattgattt ctttgctaat atccctgacc cacaatttca gttggctgtt cacaaaagcc
    11221 aatcgatagg gctctaaatc accatcatga taacttaaca tcgcaccagt tacttctact
    11281 gctgactcaa ggtgacgatg actgcgcttt ttctcctgag ttgcttcttt tttctttaaa
    11341 cgagctgata atttactcca ctttatttgt tcacttaaat cgacctcttc caaatattta
    11401 caacgcaatg cattgactga gtcactggag aatctatttt ctgtttttgc tttcaactga
    11461 tctcgattta ggctccaaat gctggaaaca gatttttcat aggcttgaat tgctttttcc
    11521 aattctctgg tttgtaagcg ctgaaaaaca tccctgcgga catagggatt tggatacatt
    11581 tttttaggat cagaatcttc caaacacttt gtccattgct tctttaacga atcaatcaac
    11641 tcgtttcttt gcacaaatag ttcattatca agctgtctcc attctgactt gggataaatt
    11701 agatgcaaaa atgcttgcca ttcttccaat tgatttaata ccgcttgttg aatattagaa
    11761 acagattgaa tgaaatactg tttaactgct tcttctcttc gtctttcagc caaactttgc
    11821 gtatttaatt cttcctgcaa attcttcctg gcagggaaaa cccgataccc cacatccata
    11881 tcgaaccggc tgatttgccc aaccacttca ccaacggttt cctctatttc ggttatcctg
    11941 acgacacgcc ccttcccttt ataataatca tacagtgaag aaaaatgaga taaagttcta
    12001 tcgtctacat tagaaaaatc aatatgatta agaataaggt agcttgagac aggatagccg
    12061 tatctatcca tagacagttc ctcatcctct atctctgcgt gcgagcgata ggaggtcaaa
    12121 tcagaaagtg ttgagtaatt aatacctcca acctggtaag taccaaactc agaattcgcc
    12181 tctaccacag aggaaggtat acccaaacag gtaaagaaat ctctatcacc cttctcaaga
    12241 taatcctgag ctattaatcc gtgatttgca aaacacacgt cgacggtacc accctcagca
    12301 agcacccatt gcatggcagc aagaattgct gcaggtgggg cgctcaggtt ttcattacga
    12361 cggacttcca ttaacacatt ttgttcttga tgatgtaaag acaataaaag aaaaatcatt
    12421 tgtgtagtat caaagaatat gccggtactg cgaaaatact gctctcgcat cactgcgagt
    12481 aacttcagtt gagttcgcag tttatcctct ttgctaatat ctagtcttct catctccata
    12541 attaaaaaat cagacaactc ctgcaaatta ttcctgccta attgcgttaa atcagtatac
    12601 gttgcactcg ctatttttag aggaaaattc ctgcctattg cattgataaa tacgacttgt
    12661 tgagccaatt catattgccg tttacacgat aaactcgctc catcagccaa actttcaatc
    12721 ttggcaataa cctcttgaat ttgacttgtg tcaaattgct cattaagaat tcgttctctc
    12781 gacttaacca gaataccttc ttgattcact ttgggggccc tggctccggc aggatctcta
    12841 tcaaatgaat caatatataa ttttaactct cttgagtatt cacgcaattt ctcggaaaaa
    12901 acatccaatg cgggataagg aggatagtca aataaagaag aaatatcttt tagtaaaaca
    12961 ctatggatct caaggagaga ctgaacattg a
//
LOCUS       DBNCWK010000089        12926 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.089, whole genome shotgun sequence.
ACCESSION   DBNCWK010000089 DBNCWK010000000
VERSION     DBNCWK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.089"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            146..1735
                     /locus_tag="AC06IO_002060"
     CDS             146..1735
                     /locus_tag="AC06IO_002060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875236.1"
                     /translation="MYTKNSLISGALNKFPGRELDEEEMLLDILWSQPNNWTALKDYL
                     NSFERIKKFAIVLFSHEKSILPELLNHIELILASSPPGAYLELIDRVFPLGTKLNDNF
                     TQWFNFSPNFFRNHVLIDGTSYHFLFERYHAFFISNPNFFESLLEKIGMPHIEQTCTD
                     FRLILCCSYDQKKIYHLFSEEFIRQWAIRVFSDVTKIFSQDTDGGHLFRKNKYPLSDL
                     DDQVAKLICGLLSDEFISLVLAQIPLRDALLQNWMEAFVKAVGPDRFMNAISKKGCNP
                     IHELISAALDMPSYETRHYSVFYSLCISISKEKFDCYIDSNEETNLDRIFKLINSLEP
                     EAYFQYKSSSLDDDISQVLINLLGKERALARHIHNMFLHGLTSKDPGKILLLESRLPD
                     ITLAEDDVDSLLCKHTIKVVQPAYKRMLLDRDSVHNIAAILRKIPESRRCEILALFEQ
                     RYDFYAYFRNHFKDYLTIKSLLPNDDHQQDGLEQKYGFSMRNELALLATSFFSGGRTQ
                     HSDAVRQSPGDEYKNNACVIS"
     gene            complement(1931..2800)
                     /locus_tag="AC06IO_002061"
     CDS             complement(1931..2800)
                     /locus_tag="AC06IO_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIO9875237.1"
                     /translation="MTVKRSSLGRNLSALLSQSSSSLLSNEQPQTEKQLKLAVTCLQP
                     GKYQPRGGMEETPLNELAQSIKKQGVLQPLLVREIDNGRYEIIAGERRWRASQLAGLT
                     EVPVILKQVDDETAMAMALVENLQREDLNAMDQARAMYRLTNEFALTHQQVADLLCKS
                     RTAVSNFLRLLSLSTPVKKLLENGDIDMGHARALLTLDEEQQSQVAQLIIAKNLSVRE
                     TEQLVERVKTGKPESKAHQKDNPQFKEQLNSLAKHLQTKIKLKQGKTGKGTLIIHYEN
                     DHNLQKIIEQLLN"
     gene            complement(2814..3584)
                     /locus_tag="AC06IO_002062"
     CDS             complement(2814..3584)
                     /locus_tag="AC06IO_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIO9875238.1"
                     /translation="MAKVIAIANQKGGVGKTTTAINLSASLAASKQQVLLIDLDPQGN
                     ATMGSGVDKRQLVHTTNDVLLRDCLAEQACLATTCGYDLIPGNEDLTVAEVSLMERNH
                     RETFLFKALQPIQSNYDFILIDCPPALNTLTINAFVAADSVLIPMQCEYYALEGLAAL
                     LSTIEQVKSTVNSRLHIEGVLRTMYDARNRLCAEVSRQLLEHFPAKVYRTVVPRNVRL
                     AEAPSHGMPALQYDKSSPGAAAYMVLAAEVISKQTVAS"
     gene            complement(3587..4213)
                     /gene="rsmG"
                     /locus_tag="AC06IO_002063"
     CDS             complement(3587..4213)
                     /gene="rsmG"
                     /locus_tag="AC06IO_002063"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIO9875239.1"
                     /translation="MIDNTKIRSLLEEGLPVFQLDSIGDLLLDFLLLLNKWNKTYNLT
                     AIRDIETMVSKHLFDSLAILPWIKGNHIIDVGTGPGLPGIPLAIAKPDLQFVLLDSNG
                     KKISFLNEVKRQLNIKNIEPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTQHLVAQ
                     DGLWLAMKGRFPDTELVPIHQTYRVERYAVPGIEGERCCVLINNTNKE"
     gene            complement(4210..6084)
                     /gene="mnmG"
                     /locus_tag="AC06IO_002064"
     CDS             complement(4210..6084)
                     /gene="mnmG"
                     /locus_tag="AC06IO_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIO9875240.1"
                     /translation="MNLEQLYDVIVVGGGHAGTEAALAAARLGVKTLLLTHNIDLLGQ
                     MSCNPAIGGIGKGHLVKEIDALDGAMAKAADQAGIQFRILNASKGPAVRATRAQADRV
                     LYRKAIRTQLQSQANLTIFQQAVDDLKIEGGLVTGVVTQMGLTLKARAVVLTVGTFLG
                     GKIHVGMNQYAGGRAGDPPSIALSKSLRDLDLPVGRLKTGTPPRIDRRTIDFSQMVEQ
                     PGDTPVPVFSYLGTASDHPQQVPCHITHTTEATHDIIRNNLDKSPMYAGVIEGVGPRY
                     CPSIEDKIVRFADKTSHQIFVEPEGLTTEEIYPNGISTSLPFEVQVQFVRTIKGFENA
                     HITRPGYAIEYDYFDPRGLTSFLQTKAIPNLFFAGQINGTTGYEEAAAQGIIAGMNAA
                     LQIKDQELWCPRRDEAYIGVLIDDLITCGTQEPYRMFTSRAEYRLLLREDNADLRLTE
                     KGRQLGLVGDERWDSFSKKREAIESTQALLHNSWVRVHHNDLFKEALLNPMQHDCRAA
                     EFLKRPEINYQHLLMMDDLNLPELPQEITEQIEIQNKYAGYIDRQQQEIEKLRKHENT
                     MLPETLDYNDVVGLSSEVIQKLNRIKPTSLAQAGRISGVTPAALSLLLVHLKKSRLPV
                     "
     gene            complement(6215..8128)
                     /locus_tag="AC06IO_002065"
     CDS             complement(6215..8128)
                     /locus_tag="AC06IO_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2888 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875241.1"
                     /translation="MATSSLILQSYLKTLKTDEKSELRYEDKVEELLEYNSRAKGGDF
                     LAPLSFYLAILENTDEVMLRYMSEENKQKLIRDLQVAYLLLLTQKKYEEEHQKYENIK
                     TYTQQIKRCEELLDYLNYNQVCKEQKRAPSPEHGYASDGYPVKYLSIFLGKELAEMIV
                     DSMDRKTKTIKESMGWFNEKRLYWVWASSLLKVILAALPDDFFNVGQATEVVKAPDPY
                     TGTLSWALYYFRFSLNMFLLLKHTISGPWMSEREKKTPWTERFLTQWDQRKFTLLNDS
                     IWATGNLICFFWLTGKGALGTWGDVLTLALLVFDISMAIWDYEEQKTRHNKEMLAYEE
                     DIKKLKQLISKAEEEPGKEDEKLRIIKEYELQLQGLMRAQKESERNWDLQKVSLYAGI
                     AYAVGLMLAFALLAAPFFPVSGPALLAITITGAVLCLAFTVIYNGVKGGMEVYKAHCS
                     AKEAKEGYNNKLELFKLLLEKNPSLDDNEKKFLFLEIKKLKAETEYQKQMVVFQTMHL
                     LRSLILESFIPAVVFASFVFLPLGIGFAVLGATIGVALATNLMINAAFKPAKNEVKAF
                     NEKEYEAFCKDPDNWDKSSKSKQGFFQPKEKKPLLDTIAAKTQEKENDRIELLDDESS
                     VPLLLSSKNTPNI"
     gene            complement(8355..9791)
                     /locus_tag="AC06IO_002066"
     CDS             complement(8355..9791)
                     /locus_tag="AC06IO_002066"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIO9875242.1"
                     /translation="MTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQQTM
                     KRAMGLPGNQTIIVSNDAHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLAN
                     TIGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNAIVTFGIKPDSPKTGYGYIE
                     AGTTLSSDIKQVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFETNI
                     YQFSQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGC
                     WTAVADANKQDEQGNTLIGNVIAQDSHNCLINSEELLVTTVGIQDQIIVATSDAVLVA
                     DKRYSQQVKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKVKRLMVKPGAKL
                     SLQTHQHRAEHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIE
                     VQSGSYLGEDDIKRFDDIYLRKEVELAE"
     gene            9881..10567
                     /gene="queC"
                     /locus_tag="AC06IO_002067"
     CDS             9881..10567
                     /gene="queC"
                     /locus_tag="AC06IO_002067"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIO9875243.1"
                     /translation="MKKAVVLLSGGLDSTTCLALAKSQGFACYALSFSYGQRHSAELC
                     AATRIAKHMGASDHKIVTLDIALFGGSALTDASIEVPEFKESPEIPVTYVPARNTIFL
                     AMALGYAESIGARDIFIGASSVDYSHYPDCRPEFIESFQSLANLATKAGIEGDRFTIN
                     APLQYLSKVQTIQLGTELGVDYGLTVSCYQANEAGEACGQCDSCTFRKRGFKSAGVDD
                     PTRYQKCVHI"
     gene            10610..11164
                     /locus_tag="AC06IO_002068"
     CDS             10610..11164
                     /locus_tag="AC06IO_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2885 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875244.1"
                     /translation="MEFFMWDKIKEWYKNNKSYLTMLLIGSAVVGLSVTFLALFFPPV
                     LAAFASISIFGMAPLAFLTSLHLPLAVLTLGCIVTGAAFGAILGGLVAWKQSISIAKH
                     VYSYFGHKQEHQVEINEEDTYRYLMGHLEGEASESFEVEVDEEFNDYTSTDSAEDRPN
                     PLNFSTDSNPQALDGGYEMTEFRI"
     gene            11474..12211
                     /locus_tag="AC06IO_002069"
     CDS             11474..12211
                     /locus_tag="AC06IO_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2884 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875245.1"
                     /translation="MTLKQFATGVFYLPAAVFSGLTNLFLGSYAKDKRGNYIQDEDGN
                     YVRNRGLIGLTLDAVKYLGTSVANFISNHQKAISVAFWSSLVVAGAAALTVAFWPAAL
                     AAVVNFSVFGVSIASVVGAGYAAQVAATAGVAAALTSTGVYLGATVVNAFNALRECIS
                     NLRSKKPSSNPEFTPVNDETFEEVRRNQFSGLSKGSEQQQTVQLSSSSSEEEEPVHTT
                     KVFVQPKDVTPEVVENPTSTVSLTGSK"
     gene            complement(12267..12836)
                     /locus_tag="AC06IO_002070"
     CDS             complement(12267..12836)
                     /locus_tag="AC06IO_002070"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIO9875246.1"
                     /translation="MANNPRLIIGISGSSGIIYGIRLLQVLQKLPIETHLVISKAGQL
                     TRAYETNISAAELKALADVYHPCTDLTASIASGSFKTLGMIIAPCSMKTLGEIAHGIS
                     SNLLTRAADVVLKERRKLVLLPRETPLHLVHLNNMVCITQMGGIICPPVPAFYNHPQN
                     IDDLINDTVGRVLDLFDLDCGLVKRWGET"
BASE COUNT         3563 a         2584 c         2645 g         4134 t
ORIGIN      
        1 aatatagtca accaagataa aatatgacca catcgtaatt aaaacaccaa aattgaatta
       61 aagataatgt tgctatatga tgcgagcgta aatggaagcc aacagccaat tcaattggtc
      121 ggcatctcgc caataaggaa atgcaatgta tacaaaaaat tctctaatca gtggtgcatt
      181 aaacaaattt cccggtagag agcttgatga agaggaaatg cttctcgata tcttgtggtc
      241 ccagccaaat aattggactg cgctgaaaga ctatctgaat tcttttgagc ggataaaaaa
      301 atttgctatc gttttatttt cacatgaaaa aagcatattg cctgagttat taaaccatat
      361 tgaacttatt ctggcatcat ccccccctgg agcatattta gaattaatag atagggtttt
      421 tcctttgggt actaagttaa atgataattt tacacaatgg tttaattttt cccccaactt
      481 ttttagaaac catgtattga ttgatggaac aagttatcat tttcttttcg aacgctacca
      541 cgcttttttt ataagcaacc cgaatttttt tgaatcattg ttagagaaaa tcggcatgcc
      601 acatattgag cagacatgta ccgattttcg attaattcta tgttgctcat atgatcaaaa
      661 aaaaatttac catctattta gtgaagagtt cattcgtcaa tgggctataa gggtattcag
      721 tgatgtaaca aaaatatttt ctcaagatac tgatggagga catctcttta gaaagaataa
      781 atatcctttg tccgatctgg acgaccaagt ggcaaaatta atttgtggat tgctgtccga
      841 tgagtttatc tcattagttt tggctcaaat accacttaga gatgctttat tgcaaaactg
      901 gatggaagct ttcgtaaaag ctgttggccc agacagattt atgaatgcta taagcaagaa
      961 aggatgtaac cctatacatg aacttatttc tgcggcactg gatatgcctt cgtatgagac
     1021 taggcattat tccgtttttt actctttatg tatcagtatt agtaaagaaa aatttgattg
     1081 ctatattgac tcaaatgaag agactaactt ggataggatt tttaaattaa tcaactcgct
     1141 ggagccagaa gcctattttc agtataagag cagctcttta gatgatgata tatcccaggt
     1201 gctgatcaat ttacttggta aagaacgagc cttggcaagg catatacata atatgttttt
     1261 acacggatta acttcaaaag atcctggaaa aatattatta cttgaaagta ggttgcctga
     1321 tattacactt gctgaagacg acgttgatag tctcctgtgt aagcatacga ttaaggttgt
     1381 tcaaccagcg tataagcgca tgctattaga tagggatagt gtacataata tagctgcgat
     1441 acttagaaaa attccagagt cgcgcagatg tgaaatattg gcattatttg aacagcgata
     1501 tgacttttat gcatacttca gaaatcactt caaagattat ttaacgatta agtcattatt
     1561 acccaatgat gatcaccagc aagatggatt ggaacaaaag tatggctttt caatgagaaa
     1621 tgaattggca ttattggcga ctagtttttt ttcagggggt aggacacaac atagtgatgc
     1681 tgttcgtcaa tcgcctggtg atgagtataa aaataatgct tgcgtgattt cttgataatt
     1741 tctttattta ggttgacaag taaaaaagga tggttcgcat gctgtcaatg tcattgaggt
     1801 gttttatata agacaagttt cagatgaagc ttcccttgat ccaatttata atagcgttta
     1861 agaatgttca ttaatccggg caggaatttt aggactgggg cctgtcatca tggttgtgga
     1921 tacaattgat ttagtttaat aattgctcaa taattttttg caaattatga tcgttttcat
     1981 aatgaataat caaagttcct ttgccggttt tcccttgttt taatttaatt ttagtctgta
     2041 aatgtttcgc taaagagttt agctgctctt taaactgagg attgtctttt tgatgggctt
     2101 ttgattccgg tttccctgtt tttactcgtt caactagctg ttccgtttct ctgaccgata
     2161 aattcttggc aatgattaat tgagcaactt gactttgctg ctcttcatcc aaagtgagta
     2221 acgctcgagc gtgtcccatg tctatgtcgc cattttccaa gagctttttt actggtgttg
     2281 aaagggaaag caatcgaaga aaattgctta ccgcagttct ggatttgcat aaaagatcag
     2341 ctacctgctg atgggtaagc gcaaattcat tggtaagtct atacatggct cttgcttgat
     2401 ccatggcatt taaatcttca cgctgcaggt tttcaaccag tgccatcgcc atggctgttt
     2461 catcatctac ctgtttgagg ataacaggaa cctcagtaag gcctgccagt tggctggcac
     2521 gccagcgtct ttctcctgcg atgatttcat atcgtccatt gtctatctca cgaaccagca
     2581 agggttgcaa tacgccttgc tttttaatgg actgggcaag ttcattgaga ggggtttctt
     2641 ccattccacc acgaggttga tacttgcccg gctgtaaaca agtcactgcg agtttgagtt
     2701 gtttttctgt ctggggttgc tcatttgata ataaagagct gcttgattga cttaataaag
     2761 cagataaatt acggcctaaa ctactgcgtt ttactgtcat tacttacccc aaattaactg
     2821 gctacagttt gtttacttat cacttcggca gcaagaacca tataggctgc tgcgcccggt
     2881 gaggatttat catattgcaa agccggcatt ccatgacttg gcgcttcagc cagtcttaca
     2941 ttgcgtggta caacggttct atacactttt gcagggaaat gttctaataa ttgcctggat
     3001 acttcggcgc ataatctgtt gcgcgcatca tacatggtac gaagcacgcc ctcgatgtgc
     3061 aaacgcgaat tgactgttga tttcacttgt tcaatggttg ataacaaagc agccaatcct
     3121 tccaaagcat agtattcaca ctgcatgggt atcaaaacgg agtcagcagc aacaaatgcg
     3181 ttaatggtta atgtattcaa agcaggaggg cagtcaatta aaataaaatc ataattagac
     3241 tggatgggtt gcaacgcttt aaataagaaa gtttcccgat gattgcgctc catcaggctg
     3301 acttctgcaa cagttaaatc ctcgttaccg ggtattaaat catatccgca agttgtagca
     3361 aggcatgcct gctcagccag acaatcgcgt aacaagacat cgttggttgt gtgaaccaac
     3421 tgtcttttat ctaccccact acccattgtt gcattaccct gaggatctaa atcgatcaac
     3481 aaaacttgct gtttggatgc ggcaagagat gcagataaat tgatggccgt ggttgtttta
     3541 cctactccac ctttttggtt ggcaatggct atgactttcg ccataattat tccttgttcg
     3601 tgttgttaat aagcacgcag caacgctctc cttcgatacc cgggacagca tatctctcaa
     3661 ctcgatatgt ttgatgaatc gggaccaatt cagtatccgg aaaccgccct ttcatggcta
     3721 accacaaacc atcctgagcg acaagatgtt gagtccattt tatcatttgt tcgagactgc
     3781 taaatgctct gcttattact gtatcaaaac cttggtttgg gtggtagttc tctactcgaa
     3841 tttgtattgg ttcaatgttc tttatattga gttgacgttt tacttcattg agaaagctaa
     3901 tttttttccc attactgtcc aataatacaa attgtaagtc aggtttggca atagccaaag
     3961 ggatgccagg taaacccggg ccagtaccca catcgataat atggtttccc tttatccatg
     4021 gtaaaatagc cagactgtcg aacaggtgct tgctgaccat ggtttcaata tcacgtattg
     4081 cggtgaggtt atatgtttta ttccatttat tgagcaggag taaaaaatcc agcaagaggt
     4141 cgccgatgga atccagttga aacacaggca gaccctcttc aagcaatgat cttattttcg
     4201 tattatctat catactggaa gccgactctt tttcagatga acaagtaaaa gagacagagc
     4261 ggcaggcgtt acgccagaaa tccgtccggc ttgagctaaa gaagttggtt taatccggtt
     4321 taatttttgg atcacttcac tcgataaacc aacgacgtca ttataatcca aagtttcagg
     4381 gagcatggta ttttcatgct tgcgtaattt ctcaatttct tgttgttgcc tgtctatgta
     4441 cccagcatat ttattctgaa tttcaatttg ttctgttatt tcttgtggca attcgggtaa
     4501 attcaaatca tccatcatta aaagatgctg gtagttaatt tctggccgct tcaaaaattc
     4561 agcggcgcgg caatcgtgtt gcattgggtt aagcaaggct tctttaaaca gatcattgtg
     4621 atggactctc acccagctgt tgtgtaacaa agcctgagtt gattcaatcg cttcacgttt
     4681 tttggaaaag ctgtcccacc gttcatcacc taccaggcca agctgccggc ctttttcagt
     4741 cagtcttaaa tcggcattat cttcacgtaa taacaaacga tactcagctc ttgaagtaaa
     4801 catcctatag ggttcttgag ttccacaagt aatcaaatca tcgataagta cgcctatgta
     4861 tgcctcatct ctgcgaggac accacaactc ttgatctttg atttgcaggg cagcattcat
     4921 acccgctata attccctgcg ctgccgcttc ctcatatccg gttgtcccat tgatttgccc
     4981 ggcaaaaaac aaattaggaa ttgctttggt ttgcaaaaat gaggttaatc cacgtggatc
     5041 aaaataatca tactcgatgg cgtaacctgg cctggtgatg tgggcatttt caaaaccttt
     5101 aatggtgcgc acaaattgca cttgtacctc gaaggggagg ctggtagaaa ttccattagg
     5161 gtatatttct tccgttgtca ggccttccgg ttccacaaaa atctggtgag atgttttatc
     5221 agcaaagcga acaattttgt cttctatcga ggggcaataa cgagggccaa ccccttcaat
     5281 cactcccgca tacatgggcg atttatccaa attatttctt attatgtcat gagtggcttc
     5341 tgtagtatgg gtaatatggc atggcacttg ttgaggatgg tcgcttgccg ttcccaaata
     5401 ggaaaatact ggtaccgggg tatcacctgg ttgttctacc atctggctaa aatcaatggt
     5461 gcgtctgtca attcgaggag gagttcctgt tttcaaacgt cctaccggta aatccaaatc
     5521 tcgcaagctt ttagataacg caatggatgg aggatctcct gctctgcctc ctgcgtattg
     5581 gttcataccg acatgaatct tgccacccag aaaagtccct actgttaata caacagcacg
     5641 tgccttgagc gtgagcccca tttgggttac aacaccagtg actaatccgc cctcaatttt
     5701 caaatcatca acagcttgtt gaaagatggt taaattagcc tgtgattgaa gttgtgttcg
     5761 aatggctttt ctatacaaaa cacgatccgc ttgtgcgcgt gtggctcgaa ccgcaggccc
     5821 ttttgaggca ttaagaattc gaaattggat ccccgcctga tcggcggctt tagccatcgc
     5881 tccatcaagg gcatcaattt ctttaaccag gtgaccttta ccaatgcccc ctatggccgg
     5941 attgcaggac atttgtccaa gcaagtctat gttatgggtc aataacaagg tttttacacc
     6001 caggcgtgct gctgcaagag ctgcttctgt accagcatgc cctccgccaa cgactatgac
     6061 atcatataat tgttcgagat tcatgtttaa ccattagtta tgccaaaatt ataaattgga
     6121 tttttggatc atctgcaaaa atgatccaag ctaacatata aaaaaatgag caattataca
     6181 cttttttggc aattgtccca agatgtaata aagattatat attgggcgtg tttttgctac
     6241 ttaacaacag gggtacactg ctctcatcat ccagcagttc gattctgtcg ttttcttttt
     6301 cctgtgtttt tgcagcgatg gtatctaaaa gaggtttttt ctctttaggc tgaaagaacc
     6361 cttgttttga tttggaggac ttatcccaat tatccgggtc tttacaaaaa gcctcgtatt
     6421 ctttttcatt aaaggctttg acttcatttt tcgcgggttt gaaagcagcg ttgatcatta
     6481 agttggtcgc cagggccaca ccaattgtgg cgcccaaaac agcaaatcca attcctaacg
     6541 gtaaaaacac gaagctggca aaaacaacgg ctggtataaa cgattccaaa atcagcgacc
     6601 gcagcaaatg catggtttga aaaacaacca tctgcttctg gtattcagtt tctgccttta
     6661 attttttaat ttccaggaag agaaattttt tttcattatc atccagtgat ggattttttt
     6721 ccaataagag tttaaatagc tcaagcttgt tattatatcc ttctttcgct tccttggcgg
     6781 agcaatgtgc tttgtagact tccataccgc ctttcacgcc attgtaaatc actgtaaaag
     6841 ccaggcaaag aacagcgcct gttatggtga ttgccagtaa agccggtccg gaaacgggga
     6901 agaaaggcgc agccagtaat gcaaaagcga gcatgagtcc gacagcataa gctatgcctg
     6961 catataaact gactttttgc aggtcccaat ttctttcaga ttctttttgt gctcgcatta
     7021 aaccttgcaa ttgcaattcg tattctttga ttattctgag cttttcatcc tcttttcctg
     7081 gttcttcttc tgccttgcta attaactgct ttaatttttt aatgtcttcc tcataagcca
     7141 gcatttcttt gttgtggcgc gttttttgct cttcgtaatc ccaaatggcc atgctgatgt
     7201 caaagactag cagtgccaaa gtcaatacat caccccaagt cccgagagcg cctttgccag
     7261 taagccagaa aaagcaaatc aaatttcctg ttgcccagat ggagtcattg agcaaggtga
     7321 attttctttg atcccattgg gtgaggaatc gttctgtcca tggtgtcttt ttttccctct
     7381 cgctcatcca ggggccacta atcgtgtgct tgagtaacaa aaacatgttg agggaaaagc
     7441 ggaaataata taaagcccag ctcagggtac ctgtataggg gtcaggggct ttgactactt
     7501 ctgttgcctg accgacattg aaaaaatcat caggtaatgc agcgagtatc actttgagca
     7561 ggctggatgc ccaaacccaa tacaaacgct tttcattaaa ccagcccatc gattctttga
     7621 tggttttggt tttacgatcc atagagtcaa cgatcatctc tgccagctct ttacccagaa
     7681 aaatgctcaa gtacttgact ggatagccat cactggcgta accatgctct ggagaggggg
     7741 ctcttttttg ttctttgcaa acttgattat aattgaggta gtctagcagt tcttcacatc
     7801 gtttaatttg ctgcgtataa gtttttatat tttcgtattt ctgatgttct tcctcgtatt
     7861 ttttttgcgt gagtaaaagc aagtaggcaa cttgcaagtc tcgtatcagt ttctgcttgt
     7921 tttcttcgct catgtaacga agcatcactt catcagtgtt ttctaaaata gccagataaa
     7981 aactcagtgg agcaagaaaa tcgcctcctt tggccctgct attgtattca agaagttctt
     8041 ctactttgtc ttcatagcgt agttcagatt tctcatcagt tttgagagtt ttcaggtaag
     8101 attgaagaat taaactactg gttgccataa agtcatctct ttttaatttt tgttaaatac
     8161 ctatataaat gatactataa aaagcaaatt tgttcttttt tataacacag catttatttt
     8221 aggtacaaga tgaatgcgat tggctttaca gtattgatac taaaatttgc aggaataagg
     8281 atgaagtgta ttgcaactta ggcaagagtg ctaagcacag tgagtggaat caagagggtt
     8341 attttcgata gacatcattc tgccagttca acttcctttc ttaaataaat atcatcaaag
     8401 cgtttgatgt cgtcttcgcc cagataggag ccactttgta cttcgataac ataaagtggt
     8461 tcattatgtg ggttacttaa tcgatgcaat gcgtttttcg gaatgtaggt ggattggttt
     8521 tttgataatc ggaacgtatt gtttccatta actacctcgg cctcaccgcc aacgataacc
     8581 caatgctcag cccgatgctg atgcgtttgt aaagagagtt ttgcaccagg cttcaccata
     8641 agccgtttta ctttaaatga ggtgccctct gccagcactt cataataccc ccatggtctg
     8701 ggaacacgtt gatgatcttg agttaaatga ggatgatctt tgcgtaagga gtgaaccaaa
     8761 tctttcactt gttgcgaata gcgtttatcg gcaactaaca cagcgtcact ggttgcaact
     8821 attatttgat cctgaattcc aacggttgtg actaacaact cttcactatt aatgaggcaa
     8881 ttgtgactat cctgggcgat gacatttcca atgagagtat tgccttgttc atcttgtttg
     8941 ttcgcgtcgg ccacagcggt ccagcagccc agatcactcc attgtattga aatgggtatt
     9001 acaacagcct tgtctgtttt ttccattatg gcatagtcta tagactcttc tttacattga
     9061 gaaaaacaat ccagatcaag acgcaaaaaa tcatgatgat gctgggcaag ggtcaatgcc
     9121 tgttgactga attggtagat gttggtttca aattgttcca gctcttcaag ataaactcct
     9181 gcacgacaaa tgaacatgcc gctattccag aagtaattcc ctttactgac aaattgtgca
     9241 gcagtgtgcg cgtctggttt ttctctaaag ctcaaaactt gctttatatc actggataaa
     9301 gtggtgccgg cctcaatgta gccataaccg gttttggggc tatcaggctt tatcccaaat
     9361 gtcactatcg cattgttttc tgaggcaaat tgtcctccct ctaacatggc ggccagccag
     9421 atttcatcat cggctatcca gtgatcagag ggtaaaacca gcatgacagc atcctttcct
     9481 attgtgtttg ccaaataatg ggctgcacat gctatcgcgg gggctgtatt tcttgcacaa
     9541 ggctcaagca agtaagtggt ttgtgcatta aaatcttgca attgatcctg acagataaag
     9601 taatgagcat cattacttac gataatggtt tgattgccag gcaatcccat cgctcttttc
     9661 atggtttgtt gaattaaaga caattcgcca tttaatttta aaaattgttt aggaaaattt
     9721 tgtcttgata aaggccatag ccgagtgcca gagcccccgg ctagaattat tggataaaga
     9781 ggagtcgtca tcacattcca tattgtggca taattggcct gcattttagc aggaattttt
     9841 aagagcaagc aattgttcca attttttgag gttttgcatt atgaaaaaag ctgtcgtttt
     9901 gctttctggt ggtttggatt caacgacttg tctcgcgttg gctaaatctc aggggtttgc
     9961 ttgttatgca ttaagctttt cttacggcca gaggcattcc gcagaactgt gtgccgcaac
    10021 tcgaatagcg aaacatatgg gtgcatctga tcataaaata gtcaccctgg atatcgcttt
    10081 atttggtggc tccgctttga ctgatgcatc tattgaggta cccgaattca aggaaagccc
    10141 ggaaatacca gtcacctatg taccagcccg taatactatt tttttggcta tggctttggg
    10201 ttatgctgaa tcaattggtg ccagagatat ttttattggt gccagttctg ttgattattc
    10261 tcattatcct gattgtcgcc cggagtttat tgaatctttc caatctttgg caaacttggc
    10321 aacgaaagca ggaattgaag gcgatcgctt tactatcaat gctcctctgc aatatctgag
    10381 caaagtgcag actattcaat taggtactga acttggtgtt gattacggat taacggtttc
    10441 ctgctatcaa gcgaatgaag caggtgaagc ttgtggacag tgtgatagct gtacttttag
    10501 aaagcggggt tttaagagtg ctggagtaga tgatcccact cgatatcaaa aatgtgttca
    10561 tatttaaccc acacagttaa attaatgata taatccctga aattttggtt tggagttttt
    10621 tatgtgggat aaaatcaaag aatggtataa aaataacaag agttatttga caatgctttt
    10681 gattggatcg gcggttgtag gtttgagtgt tacattcctg gctctgttct ttcccccggt
    10741 tctggcggca tttgccagca taagtatttt tggaatggca ccattggctt ttttaacgtc
    10801 attgcatctt cctctcgctg tgctaaccct aggctgtatt gttactggag ctgcttttgg
    10861 agcaatttta ggaggcctgg tggcatggaa acaatcaatt agcattgcta aacatgttta
    10921 ttcatacttt ggtcataagc aggaacatca agtcgagatc aatgaagagg atacctaccg
    10981 ttatttaatg ggtcatttgg agggagaggc cagtgagtct tttgaagtcg aggtggatga
    11041 agaatttaat gattatactt ctactgattc tgctgaagac agaccaaacc cattgaattt
    11101 ttcaacagat tcaaaccctc aggctcttga tgggggctat gaaatgacag aatttagaat
    11161 ctaaaattcg agcacaaatc aaatatatct tcagggttta cgaaaagcgg tgatcgcttg
    11221 aatttgatca tttggcttat tcaagcatct tagtttatgg cattttttgc ttctatcttg
    11281 ggttttcgaa aatcctgatc acctgatcat acattgtaaa ttttatcgag ctctcggcaa
    11341 aaaactggtt gagatgaaga caataaaatg aacttacttt acacttttgt aacaacatat
    11401 tgcgcacaat taattcacat ttaggaaatg ggttgctaca atctgctccg atttataacc
    11461 ttggagtaaa aaaatgacat taaaacaatt tgcaactggc gtattttatt tgccagcagc
    11521 cgttttttct ggtttaacta acctattttt aggttcttac gcaaaagaca aacgcggtaa
    11581 ctacattcaa gatgaagatg gtaactatgt aagaaaccgt ggtttgattg gtttgactct
    11641 cgacgctgtt aaatatttgg gaacaagtgt tgccaatttc atttctaatc atcaaaaagc
    11701 aatttcagtt gcgttttggt cttctttagt tgttgctggt gctgctgctt taaccgttgc
    11761 tttctggcct gctgctttgg ctgctgtagt taacttctct gtttttggag tatcaatcgc
    11821 ttcagtagtt ggtgctggtt atgcggctca agttgctgct actgccggtg ttgctgctgc
    11881 tttaaccagt actggtgttt atcttggcgc tacagttgtt aatgctttta acgcattaag
    11941 agaatgcatc tctaatttgc gtagcaaaaa gccttccagc aacccagagt ttacacctgt
    12001 taacgatgag acatttgaag aagtacgtag aaaccaattc agtggcttaa gcaaaggttc
    12061 tgagcaacaa caaactgtac aactttccag ctcaagctct gaagaagaag agcctgtcca
    12121 tactactaag gtatttgtac aacctaagga tgttactcct gaagtggttg agaacccaac
    12181 ttctactgta tctctcactg gctcaaagta atataaaaat attacaagca agtggtcatt
    12241 aaaaacgcgg ctttagccgc gtttttttat gtttcgcccc agcgtttcac caacccacaa
    12301 tccaaatcaa ataaatcgag tactctccca acggtatcat tgattaagtc gtctatgttt
    12361 tgcggatgat tataaaaagc gggcactgga gggcagataa tgccgcccat ttgtgtaata
    12421 cacaccatat tgttcagatg aacaagatgc aaaggtgttt ccctcggcaa aagcaccaat
    12481 tttctgcgtt ctttaagaac gacatcagcg gctcgggtca acaggttgct actgatacca
    12541 tgtgcaattt cacctaaggt tttcattgaa cagggcgcaa tgatcatgcc taaagtttta
    12601 aatgagccgc tggcaatgga tgcggtgagg tcagtgcagg gatgatagac atctgctaaa
    12661 gctttgagtt ctgcggcact aatatttgtt tcataagcac gggtcaattg ccctgctttg
    12721 ctaatgacca aatgcgtttc tatgggtagt ttttgtaaga cttgtaatag tctaatccca
    12781 tagattatgc ctgatgagcc actgatgcct atgataagtc ttggattgtt ggccatgtgt
    12841 attcactcgt tgaaaattta atttgatcag gatttacgac cccccaatct ctttattaaa
    12901 aacatttttt accttgacct tgtggt
//
LOCUS       DBNCWK010000090        12914 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.090, whole genome shotgun sequence.
ACCESSION   DBNCWK010000090 DBNCWK010000000
VERSION     DBNCWK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.090"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            228..1805
                     /locus_tag="AC06IO_002071"
     CDS             228..1805
                     /locus_tag="AC06IO_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 88 protein"
                     /protein_id="HIO9875247.1"
                     /translation="MKARRSNELSKLRMRFFSALNHTSEIDLHILFDNLKSNLTLGSI
                     EHLQEGSVTYAIIQELLKGEDAQKKIESFLKGAIKNVIHPGVIKGLTPDEINWNVAKA
                     YPEYYEHEKLPDVTFGGFKVRDSNEFKFKTNVQTSIWFSIKPELFMPSKQQEALKRRR
                     EQYPGCEIRLIYSSSLLNPEANRQMKAFARKQNITLIDIDTVKTDSPLYPLLKAELAH
                     LGKGGNPAAASDLCRWIPELFNEGFYVDIDLPVDSSKIVEGHQITGGVPIMLNMGSII
                     SEPIAPHHRRQEAVCMNTDIIAYSNDKRTQKMMNTVALHLKNIYDDPYTALKDTPLAQ
                     TAFFNRCKVEGKNIFELRKGLQDAFRSDSLLELYDFLGATKFKEVFKLKETQIKYIDD
                     HISEFNEHDLLLHLISDNPSEINQHTLDFGRAKVMYMDIAKEHYSAFYKPLVEEISGP
                     GAIYNALGGASNFTTTHRRSTGPMLPTTPPRVLQVFCDAHDKGPFVSDNIARWQTNVR
                     ELGVLNREGLSWLPSVG"
     gene            2010..3881
                     /gene="htpG"
                     /locus_tag="AC06IO_002072"
     CDS             2010..3881
                     /gene="htpG"
                     /locus_tag="AC06IO_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIO9875248.1"
                     /translation="MVSKQQTMGFQTEVKQMLHLVVHSLYSNKEIFLRELISNASDAL
                     DKLRFLALSNGSLFENDSDLKISIQINEKLQTITISDNGIGLSWEEAVENLGTIAKSG
                     TKEFISQLTGEQAKDSQLIGQFGVGFYSAFIVADKVTVKSRRAGLQPEDGIVWESKGD
                     GEFTIGYEKKSTRGTEITLHLKPENDEFLSDWRIRGIISKYSDHICWPIVMKKLSEEG
                     KESKEFETVNKATALWTLQKSEIGEEDYKQLYKHISHDYMDPLTWSHNHVEGKHEYIT
                     LLYIPAHAPFDLWQHEAKHGLKLYVKRVFIMDEATQFLPRYLRFIKGIVDASDLPLNI
                     SREILQDNKQVESIRAACTKRVLSMLEKMATNDKETYQKFWNEFGLVLKEGPIEDFAN
                     KEAIAKLLRFSTTASGSEKQEVSLEEYVSRMKEGQDKIYYITASSYNAAKNSPHLEIF
                     RKKGIEVLLLSDKVDEWLVGYMNEFTGKKLQSISKGKVELGDDETSEQIKEQEKTLEP
                     LIKHIKSVLNERVKDVLLTNRLTDSPACVVADEQDMGLEMQRILQAAGQQVPVSKPIF
                     EINPDHALIKRLHDIQDDNQFELWVTMLFEQAVLAEGGQLDNPADFVNRVNRLLVSS"
     gene            complement(3993..4289)
                     /locus_tag="AC06IO_002073"
     CDS             complement(3993..4289)
                     /locus_tag="AC06IO_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9875249.1"
                     /translation="MNVIDTQATTQSTKQEQGLQDLVYSLVTRFLAENKSKSINDLYD
                     MILSEVEPPLLQAVMEKRRGNQLQAAKMLGISRGTIRKKLQRYFGTKYFRLTEE"
     gene            complement(4405..5559)
                     /gene="lpxB"
                     /locus_tag="AC06IO_002074"
     CDS             complement(4405..5559)
                     /gene="lpxB"
                     /locus_tag="AC06IO_002074"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIO9875250.1"
                     /translation="MPNASRVVIVAGEESGDHHAAELVKQLKAVYPDLEISGIGGKHL
                     RAAGVHLISDLTRYAVTGLTEIIPFLKIFRKAFQDIKQHLSTQKPDLLILVDYPAFNL
                     RLAKYAKKKLGLKIIYYISPQIWAWKGKRIHLIKDCIDKMAVIFPFEKTIYENAGVPV
                     SFVGHPLVKKIAAAKDKHSSRTSLGLPLNEPIIALLPGSRHSEIERHIPILVNTAKLL
                     TLDSPKLRFVVPIAGTINPDKVKAYFSNQNLTVTFIQGQAIECMSAADFVIVASGTAS
                     LECALLEKPMCIIYKSSFLTYVAAMYFIKVKFLGLCNLLANKMMVPEFLQYDCNAIEL
                     SRYISNFHNDPNQPESMINQLAKLKESLSSSQADCSLFDLVVAELPEKNA"
     gene            complement(5546..6508)
                     /locus_tag="AC06IO_002075"
     CDS             complement(5546..6508)
                     /locus_tag="AC06IO_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIO9875251.1"
                     /translation="MNKIRCAVIGVGYLGRFHAQKYQLIPNAELVAVCDVNSTVCEAV
                     SSELNVPAYINYHDLFGKVDAVSIAATTNRHFEIAKFCLQHGIHVLIEKPITETVSQA
                     EELIRLAKQHQVKLQVGHLERFNSARLALDEYLELPLFIESQRLAPFNPRGTDVNVIL
                     DLMIHDIDIIQNIVQSPIKSITAQGAPVLTKAIDIANARISFANHCVANVTASRISFK
                     TERKTRIFQENSYISIDYQNKQLAVFKKGTGEMFPGIPDITRDQREFEKGDALLEEIR
                     AFLNCIENDSVPLVTGEEGRDALKTAEQITALINNNLVERYAEC"
     gene            6729..7304
                     /gene="rnhB"
                     /locus_tag="AC06IO_002076"
     CDS             6729..7304
                     /gene="rnhB"
                     /locus_tag="AC06IO_002076"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIO9875252.1"
                     /translation="MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKL
                     SPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVLV
                     DGAFTPQLNIPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
                     ATPVHKEALMRLGPCKIHRRSYSPVADLISK"
     gene            complement(7415..8533)
                     /gene="rodA"
                     /locus_tag="AC06IO_002077"
     CDS             complement(7415..8533)
                     /gene="rodA"
                     /locus_tag="AC06IO_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIO9875253.1"
                     /translation="MSRQTTKPVYRFTAKSLHLDFPLLGLILTLIAFGLLILYSASNA
                     NMGMIMRQSMRLLFAFLIMFVLGFIPPHKYKIWTPWIYGVGLSLLIAVMLMGKIGKGA
                     QRWLELGLFRFQPSEIMKLAVPMMAAWFFDRQSHPSSIRSIGIASLIIFIPALLIAKQ
                     PDLGTAIMVTVAGLCVVFLAGIRFKIILLIALLMCSAIPVVWNLMHDYQKQRVYTLID
                     PEQDPLGAGYHIIQSKIAIGSGGLMGKGWLKGSQSHLNFLPEHATDFIFAVSGEEFGF
                     AGGFAIVALIVLISLRSLNIANNAQTTYTRLLSASLAMTFFLSAFVNIGMVMGIIPVV
                     GIPLPLVSYGGTAMVTFLASFGILMSISSHRILFNSLN"
     gene            complement(8530..10383)
                     /gene="mrdA"
                     /locus_tag="AC06IO_002078"
     CDS             complement(8530..10383)
                     /gene="mrdA"
                     /locus_tag="AC06IO_002078"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIO9875254.1"
                     /translation="MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQI
                     SEFKRYQTLSLKNQMSVIPIAPPRGVILDRNGVLLAENIPVYVLEITPERVKDMKQTL
                     TKLRELIPSITDEDIDSFNKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVSIK
                     ARLMRHYPLGEITAHALGYVGRINIQELKQVDSTNYRATNFIGKAGIEKYYEDILHGK
                     VGYQMVETDVSGRTLRIINKVNPHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSR
                     NGEVLAMVSSPSFDPNIFVSGVSKSDYKVLSNALQRPLFNRAVRGVYPPASTIKPFVG
                     LAGLDKGFITTTTEIYDPGKYKLPTSSHVYRDWKKTGHGVINFKRAITVSCDTFFYQL
                     GNKMGISNIEDMLVKFGLGQLTHVDLHEEANGVIPSARWKRQTKGVSWYPGDTLISAI
                     GQGFMLATPLQMANATASLSQHGQRFRPHLLIKTVNSDTNETEEYKPFEEYPVSLRDE
                     ANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPVAGKTGTAQVYSGRQYEKAKYEDIPEH
                     LRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVARKVLDTYYQLYPIKKTP"
     gene            complement(10550..11020)
                     /gene="rlmH"
                     /locus_tag="AC06IO_002079"
     CDS             complement(10550..11020)
                     /gene="rlmH"
                     /locus_tag="AC06IO_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIO9875255.1"
                     /translation="MLKITIITLGNKMPDWVNSGVNEYAKRFHDGIQIKLIEIPLLRR
                     NKSSDLARILEKESALTKDALPANARLIALDMLGKSFSSVELALRLTQLQQISSHLCF
                     IIGGPEGLSNEILTLCDERWSLSKLTLPHPLVRIILLESLYRAWSIINNHPYHK"
     gene            complement(11029..11367)
                     /gene="rsfS"
                     /locus_tag="AC06IO_002080"
     CDS             complement(11029..11367)
                     /gene="rsfS"
                     /locus_tag="AC06IO_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIO9875256.1"
                     /translation="MLEKNPTLEKLLKSLEDIQAIDIKIIDVHKQTTITDFMVVTSGR
                     SSRHVKSIAQKVLEDMKVAGLPCLSSTGIEHGDWALIDFGDIILHVMQPEFRHFYNLE
                     GLWEEHPGNS"
     gene            complement(11472..>12914)
                     /locus_tag="AC06IO_002081"
     CDS             complement(11472..>12914)
                     /locus_tag="AC06IO_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIO9875257.1"
                     /translation="MEKHPKSLRIYFTTEMWERYGFYAVQSLLALYLALHFKWPDKEI
                     YSLVGSFTALTYLSPLVGGWIADKLIGQKRAILLGAIVLFLSYCMLSLIDNSLALTSS
                     LAGVAVGTGLLKPNISSLLGNEYPVGSTKRESGFTIFYMGITTGIILGTTLPSKFNEY
                     FGWSASFTSASIGLIIAFVVFLFGIHQYKIKDYNPFVFQPGKIISAFVLLVLLWSLSF
                     YILSSPQLANIMFGLVVLFSASYILYSVNRESANQSRQTLVIGLLCIISVIFWAFYFQ
                     MFMSLTLFIARVVEPSFLGIDFPPPYYITVQSIGMLIIGYFLAKKHRSLTMIERGLST
                     GKKFVLAMVFMTLAYSIIAFVSTVVDKSILISPLLIIPAYLMISLAELLLSPVGLSAI
                     TVLADKNKVSTMMGIFFVSLGIGGFLSGKLADLTAIPTGETNIIVLKTLYATAFTQQL
                     SILFIATLGCLVLFAVIKFLLTHIQICEKQ"
BASE COUNT         4127 a         2336 c         2547 g         3904 t
ORIGIN      
        1 gttcctggtt ttgattttta gaaatagtcc tgtttcttgg caaagatgta atttctgaac
       61 gatcactttt tcctgactgt aaaataaagt taatcaagaa ttttcagaat atttagagac
      121 agagtgttta taaatatccc tatatcgatt atctactata cttattcaat acgaaaatat
      181 aaatcattat ttttaagaac ggtcaacttg catgggagac agatattatg aaagcaagaa
      241 ggagtaacga actttccaaa ttgcgaatga gatttttttc agcgcttaat catacatctg
      301 aaattgactt gcatatactc tttgataacc tgaaatcaaa tcttacactt ggaagcatcg
      361 agcatctcca ggaaggctct gttacgtatg caattattca agaactcttg aaaggggagg
      421 atgctcaaaa aaagattgaa tcttttttaa aaggggcaat caagaatgtt attcacccag
      481 gtgttattaa aggacttacc cctgatgaaa tcaattggaa tgttgcaaaa gcatacccgg
      541 aatattacga gcatgagaaa ctgcctgatg tgacttttgg tggctttaaa gtacgtgata
      601 gtaatgaatt taaatttaag acgaacgtac aaacctcaat atggttcagc atcaaacctg
      661 aactttttat gccttctaaa caacaggaag cgttaaaaag aaggagggaa caataccccg
      721 gttgtgaaat tagattaatt tattctagtt cattactaaa tccagaagca aaccgacaaa
      781 tgaaggcatt tgccaggaaa caaaatatta cattaattga tatcgatact gttaaaaccg
      841 attcaccgct ttatccatta ctaaaggctg aactcgcaca ccttggaaag ggaggaaatc
      901 ctgctgccgc ctccgatttg tgtcgctgga tcccggagtt atttaatgaa ggattttatg
      961 tggatattga cctcccggtt gatagttcaa aaatagtaga aggacatcaa attacaggag
     1021 gagttccaat catgctcaat atgggctcca ttatcagtga acccatagcg ccacatcatc
     1081 gaaggcaaga ggcagtttgt atgaatacgg acattattgc ttattccaat gataaaagaa
     1141 ctcaaaaaat gatgaatacg gttgccctcc atttaaaaaa tatatacgat gacccttata
     1201 ctgcattaaa agacacacca ctcgcacaaa cggctttttt taatagatgc aaagtggaag
     1261 ggaaaaatat atttgaatta agaaaaggat tacaagatgc ttttcgatcc gattctttat
     1321 tggagttata tgatttttta ggggctacaa aatttaaaga agtatttaaa ttaaaagaaa
     1381 ctcaaatcaa atatattgac gatcacatta gtgaatttaa tgagcatgat ctcttgttac
     1441 acttaatatc tgataatcct tctgaaatta atcaacatac tcttgatttt ggccgagcta
     1501 aagtgatgta tatggatatt gctaaagagc actattctgc attctacaaa ccattggttg
     1561 aagagatttc aggcccaggt gccatttata atgctttagg cggagcgagc aatttcacta
     1621 ccactcatag gcggtcaacg ggacctatgt tgccaacaac cccaccacgt gttttacagg
     1681 tattttgtga tgcgcatgac aagggtcctt ttgtttcaga taatattgcg cgttggcaaa
     1741 ccaatgtccg agagctgggc gtgttgaatc gagagggttt gagttggttg ccttcagtag
     1801 ggtagcttaa tttcaacaca caaattttga atattgtctc aagcattagc aatataaata
     1861 atcaccaata gataaaagac tttattggtg attggtgttt ttttgctggt tttctccttt
     1921 tttagcgcta tccccttgaa attcaataga tcaaccctat atgagaaggt aattattacg
     1981 aacacaacta ttaacgggga gattgtttaa tggttagcaa gcagcaaact atgggatttc
     2041 aaacagaagt aaagcaaatg cttcatttgg ttgtgcattc tctatatagc aacaaagaga
     2101 tttttcttag agaattaatt tctaatgctt ctgatgcctt ggacaagtta cgatttttag
     2161 ctttatcaaa cggttcattg tttgaaaatg attctgattt gaaaattagc atccagatta
     2221 atgaaaagct tcaaaccatt accatcagcg ataatgggat tggtctgagt tgggaagagg
     2281 cagtagaaaa tttggggaca attgcaaaat ccggaacaaa agaatttatt agccaattaa
     2341 ctggtgaaca agcaaaggat tctcaactaa taggacaatt tggagtgggt ttttattccg
     2401 cgtttatcgt tgctgataaa gtaacagtta aaagtcgccg cgctgggtta caacctgaag
     2461 atggaattgt atgggagtct aagggtgatg gtgaatttac tataggatat gaaaaaaaat
     2521 cgacccgcgg tacagaaatt accttgcact taaaacctga aaacgatgag tttttaagtg
     2581 attggcgtat tcgcggtatt attagtaaat actctgatca tatttgttgg cctattgtga
     2641 tgaaaaagct ttcagaggaa ggcaaggaat caaaagaatt tgaaacagta aacaaggcga
     2701 cggcactttg gacactacag aaatctgaaa ttggcgaaga agactataag caactgtata
     2761 aacatatttc tcatgattac atggatccat taacctggtc tcataaccat gttgaaggaa
     2821 aacacgagta tattactttg ctttacatac cagcccatgc cccatttgat ttatggcagc
     2881 atgaagccaa acatggacta aagctttatg taaagcgtgt atttattatg gatgaagcga
     2941 cacaattttt accccgatat ttacgattta taaaagggat tgtggatgcc agtgatttac
     3001 cgcttaatat ttctcgagaa atattacagg ataacaagca ggtagaatct attcgagctg
     3061 catgcaccaa acgtgttttg tctatgcttg aaaaaatggc taccaatgat aaagaaacct
     3121 atcagaagtt ttggaatgaa tttggacttg tgctaaaaga aggacctatt gaggatttcg
     3181 ctaataagga agcgattgcc aagttgcttc gtttttctac tacagcaagc ggtagtgaga
     3241 aacaagaagt atcgctagag gaatatgtta gtcgcatgaa agaagggcag gataaaattt
     3301 attatattac cgcatcgagc tacaatgctg ctaaaaatag tcctcatttg gaaatattca
     3361 ggaaaaaggg aattgaggta ttattgctca gtgataaagt cgatgaatgg cttgtaggtt
     3421 atatgaatga atttacaggc aaaaaacttc agtcaatttc aaaaggcaag gttgaattgg
     3481 gcgatgatga aacttccgaa caaattaaag aacaggaaaa aactctggag ccattgatta
     3541 agcatattaa atcagttctc aatgagcgag ttaaagatgt attactgact aatcgtctta
     3601 ctgactcccc tgcatgtgtt gttgctgatg agcaagacat gggattggaa atgcaaagaa
     3661 ttttacaagc tgcgggtcaa caggttccag tgagcaagcc tatttttgaa atcaatccgg
     3721 atcatgccct gattaagcgg ttgcatgata ttcaggatga taaccaattc gaattatggg
     3781 ttactatgct gtttgaacaa gctgtgttgg cagaaggagg gcaattggat aatcccgctg
     3841 attttgtgaa tcgggttaac agactcctgg tttcctccta gaattctata gggttggcaa
     3901 tcaatcccgg attagaacaa ctcattatcc gggatgagtt gaacataaaa taaaaaggct
     3961 caaaaatgag cctttttatt tttaaatttc ccttactctt cagttaagcg aaaatatttg
     4021 gtaccgaaat atctttgtaa tttctttctg atcgtaccgc gactaatacc caacatcttg
     4081 gctgcttgta attgatttcc acgacgtttt tccattacag cttgtaatag aggtggctca
     4141 acttctgaca ggatcatatc atagagatca ttaattgatt tggatttatt ttcagcaaga
     4201 aagcgggtaa ccaaactgta aactaaatcc tgtaaacctt gttcttgttt agtggattga
     4261 gttgtagctt gtgtatcaat gacattcatc ttaacttcct tattatttaa ttaaatcata
     4321 cagtccaatt gcttacacat gtaaatgaaa gcaaaattaa ttgtacatga aggctaaatg
     4381 ataatctaga ttttcatatc aaaactatgc atttttttca ggcaattctg ccactacaag
     4441 atcaaagaga gaacagtcag cctggctgga agacagagat tcctttaact ttgccagttg
     4501 attaatcatc gactcgggtt ggttaggatc attatgaaaa ttagaaatat atctagataa
     4561 ttcaattgca ttgcaatcgt attgcaaaaa ttcaggtacc atcattttat tggctaacag
     4621 attacatagg cccaaaaatt taactttaat aaaatacatg gcagcaacat aagtaagaaa
     4681 ggacgatttg taaataatgc acattggttt ttccaataag gcacattcta atgaggcagt
     4741 tccagaagct actatgacaa agtcagcagc agacatgcat tctattgcct gtccctggat
     4801 aaaagtaaca gtaagattct ggttgctgaa ataagctttt actttatcag gattgatagt
     4861 tccagcgata gggactacaa atcgaagttt ggggctgtct agggtcagca attttgctgt
     4921 attcaccaat atcggtatat ggcgttcaat ttcagaatgg cgactcccag gcagtaaggc
     4981 aataattggc tcatttaaag gtaatcccaa ggaagttctg ctcgagtgtt tgtccttggc
     5041 agcagctatc ttttttacta aaggatgtcc tacaaaactg acaggaacac ctgcattttc
     5101 atagatagtc ttttcaaatg ggaagatgac agccatttta tcaatgcaat ctttaataag
     5161 atggatacgt ttgcctttcc atgcccatat ttgagggctg atgtaataaa tgattttgag
     5221 ccccagtttt ttctttgcat acttggccaa tctcaaattg aaagcagggt aatctaccaa
     5281 aatcagcaaa tcaggttttt gtgtgctcag atgctgtttg atgtcctgaa aggctttacg
     5341 gaatattttc aggaaaggaa ttatttcagt caaacccgtt acagcataac gcgttaaatc
     5401 tgaaattaaa tgaaccccag cggctcttaa gtgttttccg ccaataccac taatttccag
     5461 gtccggataa actgctttta attgctttac cagctcggca gcatggtggt caccagattc
     5521 ttcgcctgcc actatgacaa cacgactagc attcggcata gcgttccaca aggttattat
     5581 taattaaagc ggtgatttgt tcggctgttt tcaaagcatc ccttccttct tcacctgtaa
     5641 caagcggcac gctatcattt tctatgcaat ttaagaaagc tctgatttcc tccagtaacg
     5701 catctccttt ttcaaactcc ctttgatctc tggtgatatc aggtatccct gggaacattt
     5761 ctcccgtgcc ttttttaaat acggcaagct gtttgttttg gtaatcaatt gaaatataag
     5821 aattctcctg aaagattctt gtttttcgtt ccgttttgaa actgattctg ctggctgtaa
     5881 catttgctac gcagtggtta gcaaagctta tgcgtgcatt agctatatca attgcttttg
     5941 ttaataccgg tgctccttga gccgtaatgg atttaatagg gctttgaact atattttgaa
     6001 taatatctat gtcgtgaatc atcaaatcaa gtatcacgtt tacatcagtt ccacgggggt
     6061 taaaaggtgc gagcctctgc gattctataa ataatggtaa ttccagatat tcatccaggg
     6121 caaggcgagc agaattgaag cgttctaaat ggcctacttg taacttcacc tgatgttgtt
     6181 ttgcaagtcg aatcagttcc tcagcctggg acactgtttc agtaatcggt ttttcaatca
     6241 gaacatgtat cccatgttgt aaacagaatt ttgcaatttc aaaatgcctg ttggtagtcg
     6301 ctgcgatact gacagcgtct actttgccaa acaggtcatg gtaattgatg tatgccggaa
     6361 catttaactc actggatacc gcttcgcaga ctgtagagtt tacatcacaa actgcaacta
     6421 actctgcatt aggaattagt tgatattttt gggcatgaaa cctgccaaga taaccaacac
     6481 caataactgc acaacgaatt ttattcatct catcatcaat tgataattat ttgtacttat
     6541 gatcgcattt ccttagtgat aaatcaattg tatttcctat gttatcatgc cagttatata
     6601 gtaattgagg catttaaaac tccaatcaaa ctcctgaaat tagttcaaat caaagaagtc
     6661 gattcttgtc ttgcacttgc attcaatagc tattatctta cgcctaattt ttcgcagaga
     6721 gcagttaaat gaatactgag ctaaaaatat tgatggctgg ggtcgatgaa gtggggagag
     6781 ggccacttgc gggtgccgtt gtaactgcag ccgttatttt aaaaaaacca atagatgggc
     6841 ttaccgactc aaaaaaatta agcccaaaac aaagaaattt attagcaatt agaataaagg
     6901 aagaagcgtt agcttttgcc tatgggcgag cagaagttga ggaaatagat caattaaata
     6961 ttcatcatgc tactctatta gctatgcgaa gggcagttga agctttaccc attcagccag
     7021 ataatgtcct tgtcgatgga gcttttactc ctcaattgaa cataccatgc aaggctattg
     7081 tccagggaga tagcttgata cctgaaatta gtgcggcatc catcctcgcc aaggtattgc
     7141 gtgatgaaga aatggtagct cttgataaaa tatatccagg atatggtttt gcagaacata
     7201 aaggttatgc aacccccgtt cataaagaag cgctaatgcg tttgggtcct tgtaaaatac
     7261 atagaagaag ttactctcct gttgcggatt tgatttcaaa gtaaaattct gagtggtatt
     7321 aatttggaaa ataccgttca aataaaaaca cataagaaat agcttttaaa atgtcataat
     7381 atgatttatg caaatcagag tataaaaaaa taaatcaatt caagctatta aataatattc
     7441 ggtgcgagct tatcgacatt aaaattccaa agcttgccag gaaagttacc atagctgttc
     7501 caccataact gacgagcgga agaggtatac ctacaaccgg aataatcccc atgaccatac
     7561 caatattgac aaatgcagaa aggaaaaagg tcatggccag actggctgaa agcaggcgcg
     7621 tgtaagtggt ttgtgcatta ttggcaatat ttaaactgcg tagcgaaatt aagactatta
     7681 gagcgacaat ggcaaatccg ccggcaaagc caaattcttc gccacttacc gcaaaaataa
     7741 aatcagtagc atgttctggc aagaagttga ggtgtgattg actacccttt aaccatcctt
     7801 tgcccattag gccgcctgag ccaatggcaa ttttggattg aataatgtga taaccagcac
     7861 caagtggatc ttgttcagga tcaatcaggg tatacactct ttgtttctgg tagtcatgca
     7921 tcagattcca cacaacagga attgctgaac acatcagtaa tgcaattaat aatatgattt
     7981 taaaacgtat tcctgccaaa aatacgacac ataaaccggc aacagtaacc ataattgcag
     8041 tacctagatc aggttgtttt gcaataagaa gagccggaat aaaaataatc agggaagcaa
     8101 taccaattga gcgtatgctg cttggatgag attgtcggtc gaaaaaccag gcggccatca
     8161 taggtacagc cagtttcata atctcggaag gttgaaaacg aaataatccc agttctaacc
     8221 aacgttgagc tcctttacca attttaccca ttagcatgac tgcaattaac aggcttaacc
     8281 caaccccata aatccatggg gtccagattt tatatttatg gggcggaata aatcccagta
     8341 caaacatgat tagaaaagca aagagcagcc tcatggattg gcgcatgatc atgcccatat
     8401 tggcatttga agcgctgtat agtatcaaga gtccgaaggc aataagagta agtattaatc
     8461 ccagtaatgg aaaatccagg tgaagggact ttgctgtaaa acgatatacg ggcttagtcg
     8521 tttgtctact catggtgttt tcttaatagg ataaagttga taataagtgt caagcacctt
     8581 acgtgctaca gtggaagcga tggtatcatt ttcaactacc acggcaatgg caatttccgg
     8641 tttttctaca ggagtaaatg caataaataa cgaattatcc ctcaagtgct caggtatgtc
     8701 ttcgtatttt gccttttcat attgtctacc actgtatact tgtgctgtac ctgtttttcc
     8761 tgctaccggg tagggaggat ttctgccaaa acgataaccg gttccttcat tgctggtaag
     8821 cacgctatgc atcgcatcaa taacaacatc ccaatttgct tcatctctca gggaaactgg
     8881 atattcttca aatggtttgt attcttcggt ctcattagtg tcgctgttta ctgtttttat
     8941 cagtaggtga ggtctgaatc gttgtccatg ctggcttagg gaagctgtgg cattagccat
     9001 ttgcaaaggg gttgccagca taaatccttg tcctattgca gaaatgagag tatctccagg
     9061 ataccaggat actcctttag tttgtctttt ccagcgagca cttggtatta ctccatttgc
     9121 ttcttcatga aggtcaacgt gtgttagttg tcccaaacca aacttgacaa gcatgtcttc
     9181 aatgttagag attcccattt tattacctag ttggtaaaaa aaagtgtcac aagagacggt
     9241 aatggctcgt ttgaaattaa tgaccccgtg gcctgttttt ttccaatcac ggtaaacatg
     9301 gctggaagta gggagtttgt actttccggg gtcatatatt tcagtggtcg tggtaatgaa
     9361 gcctttatcc aaaccagcca gtccaacaaa cggtttgatt gttgatgcag ggggataaac
     9421 acctcggact gctcgattaa aaagagggcg ctggagggca ttggataaca ctttataatc
     9481 cgatttactg actccactga cgaaaatatt gggatcaaaa cttggagaac tcaccatcgc
     9541 aagtacttcg ccattccgtg agtttatgac tactacagca cctcgtttat ccttcaacgc
     9601 attataagct gcttcctgaa gtctggcgtc tatacttaaa tacagtttcg ctcctgaatg
     9661 aggattgact ttatttatta ttcttaaagt gcggccacta acatcagttt ctaccatttg
     9721 ataacccact ttaccatgga gaatatcttc atagtatttt tcaataccgg ccttgccaat
     9781 aaagttggta gcacgataat tagtggagtc cacttgtttt agttcctgaa tattaatgcg
     9841 gccgacatac cccaaggcgt gggcagtaat ctctccaaga gggtaatgac gcatcaatct
     9901 cgctttaatg cttactcccg ggaaatgata ctgattgctg gcaaaggtag ctacttcttc
     9961 ctggcttaat tttaatttga taggaatggg aacaaaagaa cgattttgta atctggtttt
    10021 attgaagcta tcaatatctt cgtctgtaat cgaaggaatt aactctcgaa gtttagtcaa
    10081 agtctgtttc atgtctttaa cacgttcggg agtgatttcc agcacataca ctggtatgtt
    10141 ttcagcgagt aatactccat ttctatccaa aataacccct cttggaggag caataggtat
    10201 tacgctcatt tgatttttta aagacaaggt ctgataccgt ttaaattcgg atatttgcag
    10261 gaaggccaat cttaaaatca gtattaaaga taaaataatc agaatcgcaa ttaacagatt
    10321 aagtctaaat cgttgatgtt gggattcagc acgatagttt ttaaatgatt gattccgata
    10381 catcgcagaa aatgttgtat gacaaaacaa aagtaggaat agtacctgaa attacagatt
    10441 tttaccaatt ataaaatatt tataaagctt gtggtttttc attttttgag attagagtca
    10501 aatttttcta tgtaatatag aaagagtgtt gcaaaagact atgcacaccc tatttatgat
    10561 aaggatggtt gttaatgata gaccatgctc tatagaggga ctccagcaaa ataatgcgaa
    10621 ccagaggatg aggaagggtt aatttggata atgaccagcg ttcatcgcaa agagtcagta
    10681 tttcattgga taatccttct gggccgccaa ttataaaaca aaggtgactt gaaatttgtt
    10741 gtaattgcgt caacctaagt gctaattcca cgctgctaaa ggattttccc agcatgtcca
    10801 aggcaataag gcgagcattg gcaggaaggg catctttagt caaagcactt tctttttcta
    10861 gtattcttgc taaatccgag gatttattac gacgcagcaa agggatttcg atgagtttta
    10921 tctggatgcc atcatgaaac ctctttgcgt attcgttaac tcctgagtta acccagtcgg
    10981 gcattttatt gccaagagtg ataatggtaa tttttagcat agataatgtc aggagttacc
    11041 agggtgttct tcccataatc cttcaaggtt ataaaaatgt cggaattctg gttgcatgac
    11101 atgcaatatg atgtctccaa aatcaattaa agcccaatca ccatgttcta ttccagtaga
    11161 actcaagcaa ggcagaccag caaccttcat atcttccaga actttttgag ctatcgattt
    11221 gacatgtctg gatgaacggc ctgatgtgac gaccataaaa tcagtgattg ttgtttgttt
    11281 atgcacatca atgatcttaa tatctatcgc ttgaatgtct tcaagagatt ttaacagttt
    11341 ttcgagcgta ggattttttt caagcataac taagatatat tccgtcaaaa gggttgaata
    11401 atatcaacaa ttgatttgta taaaaagggt tttagttttc aagtctggaa aactaaaacg
    11461 taaaaatcta attattgttt ttcgcatatc tgaatatgag tcaataaaaa tttaattaca
    11521 gcaaacaata ccaggcagcc taacgtagca atgaataata tactgagttg ttgggtgaat
    11581 gctgtagcgt agagcgtttt taaaacgatt atatttgtct ctccagtagg aatggcagtc
    11641 agatcggcta attttcctga aaggaatcct cctattccta atgaaacgaa aaagataccc
    11701 atcatggtgc ttaccttgtt tttgtcagca agtacagtta ttgctgacaa ccctactggt
    11761 gaaagcagta attcagccaa agaaatcatc aggtaggcag ggatgataag aagaggtgaa
    11821 atcaaaatgg atttatcaac tacagtactt acaaaggcaa taattgagta ggcgagtgtc
    11881 atgaatacca tagcaagtac aaattttttc ccggtactta agcctctttc aatcattgta
    11941 agactacggt gctttttggc aagaaaataa ccaattatta acataccaat actttgcact
    12001 gtgatgtagt atggtggggg aaaatcgata cctaaaaagc ttggctcgac tactcgcgca
    12061 ataaataatg ttaaggacat gaacatctga aaataaaatg cccagaaaat aacagagatg
    12121 atgcacaata acccgataac taatgtttgc ctggactgat ttgcactttc acgatttact
    12181 gaatataaaa tgtaactggc tgaaaaaaga accacaagtc caaacatgat attcgccagc
    12241 tgaggtgaac ttaaaatata aaatgacaat gaccacagca aaaccaataa cacaaaagct
    12301 gaaatgattt tacctggttg gaaaacaaaa ggattataat ccttaatttt gtactgatgt
    12361 attccaaata aaaacactac aaatgcgatg attagtccga ttgatgcact ggtgaaggaa
    12421 gcagaccagc caaaatactc attaaacttg cttggtagtg ttgttccgag gattattcct
    12481 gtcgtgatgc ccatgtaaaa aatagtaaaa ccactttctc tttttgttga tcctactgga
    12541 tattcattac ctagtaatga agaaatatta ggttttaata aacctgttcc tacagctact
    12601 cctgcaagtg aagaggttaa tgccagcgaa ttatcaatca atgacaacat gcaataactt
    12661 aaaaacagaa caatagcacc cagcaatata gcgcgttttt ggcctattaa tttatcagca
    12721 atccagcctc ctactaatgg tgagaggtag gtgagtgctg tgaacgatcc tactaaagaa
    12781 tagatttctt tgtccggcca tttaaagtgt aaggccaaat aaagtgctaa taaggattga
    12841 actgcgtaaa aaccataacg ttcccacatt tcggttgtaa agtagatgcg gagagatttt
    12901 gggtgttttt ccat
//
LOCUS       DBNCWK010000091        12843 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.091, whole genome shotgun sequence.
ACCESSION   DBNCWK010000091 DBNCWK010000000
VERSION     DBNCWK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12843
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.091"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            118..1311
                     /gene="alaC"
                     /locus_tag="AC06IO_002082"
     CDS             118..1311
                     /gene="alaC"
                     /locus_tag="AC06IO_002082"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIO9875258.1"
                     /translation="MSQFPRIKRLPPYVFNTINQLKMEARARGEDIIDFGMGNPDQPT
                     PPHIVNKLIEVAQRPDTHRYSMSKGIPRLRRAMATWYLRHYDVHLDSEKQILATIGSK
                     EGLAHLALAISGPGDTVLVPDPAYPIHTYGFIIAGANVKQIPLIDEVQFLAAVEDAIN
                     RTWPKPKALVLNFPANPSTHCVEYEFFEKVVDLAKRNNIWIIHDLAYADIVFDGYKAP
                     SILQVPGAIDIAIETYSMSKSYNMPGWRVGFACGNEELVAALTRIKSYLDYGTFTPIQ
                     VAAITALEGPDDCVLEIRNLYEKRRNLLCDGLNDIGWEVSKPKGTMFVWAPIPFNYRA
                     MGSLEFSKYLLKEAQVAVSPGIGFGQQGDGYVRFGLIENKDRIRQALRNLKALFKRDG
                     LLKAI"
     gene            1316..2125
                     /locus_tag="AC06IO_002083"
     CDS             1316..2125
                     /locus_tag="AC06IO_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875259.1"
                     /translation="MDVIINTESGCDSKEGKLLKSQGLAVLNLLACGGYDLVNPPVGN
                     LLKSSHNLEGDWVVLTPMHWQASHNDAVIVALDKDLRVTDEEVKYWFDLYSAYLAEDG
                     MPLYFYDKYTWLLRVDDKPPLNAKPIYQVLNKSLMPELSQLDETMYWQKFFTESQMFF
                     SSNARKSLINGVWAWGSGKLKTKKTISICTDKHFLAVAQAYSSNVTLYDPSVNVSGFD
                     VVLLHGIDSLSELHQVEIKKIPAHWYWNNCVLIKAKSHWFTRLWRSLTHAD"
     gene            2115..3854
                     /gene="recJ"
                     /locus_tag="AC06IO_002084"
     CDS             2115..3854
                     /gene="recJ"
                     /locus_tag="AC06IO_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIO9875260.1"
                     /translation="MLIKQRQQPEFTVNLPNVPDVLKRIYITRGISDESQLDKQLQTL
                     LPFHTLKGINEACLRLENALRSEQRILIIGDFDADGATSTALAITALRAMGAKFVEYL
                     VPNRFEFGYGLTPAIVEVASKWQPHLIITVDNGIASFEGVDAANKMGIDVLITDHHLP
                     AESLPDACAIVNPNQPGCLFPSKSIAGVGVIFYVMLALRRHLSNCNWFNEINIPEPNM
                     ASFLDLVALGTVADVVGLDQNNRIMVNQGMMRIRQGQCREGIKALIEIAGRDYSRLRE
                     SDLGFAIAPRLNAAGRLDDMSLGIECLITTDPKMARNFCQQLDELNLERRQIESEMKE
                     QAMLALEKLSINDEENKHLPVALCLHDKTWHQGVIGILAGRMKEKYHRPVIAFASVSD
                     HELKGSARSVPDLNIRDVLAAVDKDNPGLILKFGGHAMAAGLSISPELLNDFRSAFVS
                     EVLKHLDLSQCEGEILTDGPLQSHELSLETAQLIQQAGPWGQAFAEPVFDNIFEILDQ
                     RIVGKNHLKMTLISSQGGEPLDAIAFNVDLKSWPNHRIKYIHAAYKLDINFYQGRTRL
                     QLLIQAMNPVQQA"
     gene            3933..4925
                     /gene="dusA"
                     /locus_tag="AC06IO_002085"
     CDS             3933..4925
                     /gene="dusA"
                     /locus_tag="AC06IO_002085"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIO9875261.1"
                     /translation="MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGA
                     VQNNPDRALYFNSVEHPIAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQA
                     GRFGACLMKEPGHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGC
                     DKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINSH
                     LKHVDGVMLGRLACDNPYQIAEIHQALYPEFIKIKRSQLLINYLDYWFDQSRANLSLS
                     ILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQYLLNLEAINQVEFV"
     gene            5026..7716
                     /gene="secA"
                     /locus_tag="AC06IO_002086"
     CDS             5026..7716
                     /gene="secA"
                     /locus_tag="AC06IO_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIO9875262.1"
                     /translation="MLSTLIKKMFGSRNERTLRRMEKSVMAINAFEPKMQALSNEELA
                     GKTQEFKERFNNGESLDELLAEAFATVREVSLRTLGLRHFDVQLIGGMVLHEGNIAEM
                     RTGEGKTLVATLPAYLNAISGRGVHIVTVNDYLAKRDSQWMKPIYEFLGLTVGVIYPD
                     MSHKEKQEAYKADIVYGTNNEYGFDYLRDNMAFSLTDKVQRELNFAIVDEVDSILIDE
                     ARTPLIISGAAEDSSELYIKINSLIPQLKKQEEEGDEGDYTIDEKQKQAHLTDAGHLH
                     IEELLTKAKLLDPGESLYHASNIMLMHHVNAALKAHAMFHRDIDYIVKDNQVVIVDEH
                     TGRTMPGRRWSEGLHQAVEAKEGVPIQNENQTLASITFQNFFRMYNKLSGMTGTADTE
                     AYEFQQIYNLEVVVIPTNRSMIRKDEADLVYLTQADKFQAIIEDVRECGVRRQPVLVG
                     TVSIEASEFLSQLLKKENIKHQVLNAKFHEKEAQIIAEAGRPGAVTIATNMAGRGTDI
                     VLGGSLAADLANLPADASEQEKEVVKKEWQKRHDEVIAAGGLRIIGSERHESRRIDNQ
                     LRGRAGRQGDPGSSRFYLSLEDNLMRIFASERVASMMRRLGMQPGEPIEHSLVTRAIE
                     NAQRKLEGHHFDVRKQLLDYDNVANDQRQVIYTQRSSIMAMTDTQEVVEMMREEVMDS
                     LVDTYIPPQSLEDQWDPQALSDVLSDEFKIKAPVPDWIDKDHSIQPEQIKEKILALAI
                     EHYDEKVRKVGRPVISQFEKSIILQTLDNHWREHLAAMDQLRQGIHLRGYAQKDPKQE
                     YKKEAFSLFTMMLDNLKYEVIRILSSVEIQTEEDAHVVEEQRRADQIKKMNLMHENLS
                     ENDEASETQTFRRQEKKIGRNDPCPCGSGKKYKACHGSLV"
     gene            7713..8117
                     /gene="mutT"
                     /locus_tag="AC06IO_002087"
     CDS             7713..8117
                     /gene="mutT"
                     /locus_tag="AC06IO_002087"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HIO9875263.1"
                     /translation="MKVAVAIIIDEKQRILITQRPFHVAHGGFWEFPGGKLEPHESPE
                     DALVREIREELGVIVNEYRFLGYVDYDYPDKHIQLIIFMVTRFTGNPLCQEGQLYMKW
                     VKKEELNINDFPKANHAVFDLIDIPELQYQAL"
     gene            8346..9164
                     /locus_tag="AC06IO_002088"
     CDS             8346..9164
                     /locus_tag="AC06IO_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIO9875264.1"
                     /translation="MITKQNGIYTLTLSLLLFSLQIKAATDYQTYETVCGEPTDMLSF
                     LERGGIATNPCVVPPRSVLISSGYQYQQLIGEGVQHNFPAAAIQLGLPGYFEVDLLLP
                     NYINQTVDPRIGFSQTQLIVNHVLWFNNKWVITASGTFVFPSGSASFGSPNPGGGVIG
                     ILSYNFNSQLNLTGNLGITSQSEPSYDGGQSYTSVNPDLILSWTKNKISLFAEIYGQS
                     KTAPDEGSGFSTDAGVLYQVKKNIAIDFEVDQRITGLLNGVERYYGGGITIQFN"
     gene            complement(9208..9954)
                     /gene="zapD"
                     /locus_tag="AC06IO_002089"
     CDS             complement(9208..9954)
                     /gene="zapD"
                     /locus_tag="AC06IO_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HIO9875265.1"
                     /translation="MHDHMITFQLATHFLSRIALRLEYLFKTINEACNESHEVIHRFA
                     LKNIIEIAEIVEKPELKSRFIKELIRIEHVLKKANLLNHSELFDDLETQIHVLNHVPG
                     RFSNKIHDDEFLKTLRQIHHPNTKECEFNSPHLVLWFDSDPLMRQKTISQWVSCLKDL
                     EDTVKIYLSLLRGVTQYTPIRANNGFYQHSLSPKSLNHLILLRIDKKLKMIPKIQLGH
                     HSLTIRLYELVTGQEIRDKTIEMEIAFCQI"
     gene            complement(10192..10797)
                     /gene="coaE"
                     /locus_tag="AC06IO_002090"
     CDS             complement(10192..10797)
                     /gene="coaE"
                     /locus_tag="AC06IO_002090"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215598.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIO9875266.1"
                     /translation="MVYSVGLTGNIASGKSTVAEFFSELGINVIYADKIAKELTSKDT
                     PCYQDIISHFGSSVVLNNGELDRKRIRDIIFSNSNERLWLESLLHPVIRKKIEEQLIV
                     CTSPYCLIEIPLLFNKHHYPYLQKVLLVIAPLESQLDRIVKRDHCTKKQALAILATQP
                     NLEQRLEAADDVLINESGLSELKAKVNKLHQKYLREAKIKQ"
     gene            complement(10769..11242)
                     /locus_tag="AC06IO_002091"
     CDS             complement(10769..11242)
                     /locus_tag="AC06IO_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875267.1"
                     /translation="MRNYIFYGLLLLIGLSHQGFTAPMMKKGSYWKCVTYDKANKAWT
                     AQSSYRKVAINVAFAACKKESQLPATCKTSISNCEGFINGVSTRPMWRCTAIDITAQP
                     WESNFYSNRDDAALAAQAYCKENSTLPATCYINMVTCANKNEGAHSDGLFSGTNW"
     gene            complement(11239..12837)
                     /locus_tag="AC06IO_002092"
     CDS             complement(11239..12837)
                     /locus_tag="AC06IO_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIO9875268.1"
                     /translation="MSKVIHKLSLFLFKKFTIIWILLTIGILISAFYANWNINQKRAY
                     KDLKEVAINLSNNIDGFIEDLLQEIYALPVYGKKIVDCKSDLYPFLEHITLNNPKISG
                     VIISDNKHNLFCSTLPNNKDLISTPIRSRSIIGPYNLPLFDQPVYLIQQKMGNYLIGI
                     LVIASVLKSELQTDKNQSTSIALYNEYEKKNIFRIRYTEQNKNWVLSNTQETDTQYTP
                     LGLVAIEKLQSINGVSVVVSENNKTIRHNFWYGQTITIFIVLICSFILYALIKNMMTK
                     RYSLQGAIKLAIKNEEFYPVYQPLFDCDEERFTGVEVLLRWENEDSQIIMPDFFIAEA
                     EATGLIVPITLQIIEIAFKEFRSLLEERSYFHLAFNISALHFTDSVFFNQFHKLIEKY
                     NISPHQIIFEITERELLDKNDTTFINKMQELRQAGFSLAVDDYGTGHASISYLQHFPF
                     NYLKIDKLFVQAIGSNDIIESLNDAIIQMAKGLNLVTIAEGVETKDQANYLANNGVRL
                     QQGWYYSKGLSIAELTVLLKGEKI"
BASE COUNT         4042 a         2112 c         2631 g         4058 t
ORIGIN      
        1 tttttatcgc tgtaagcttg cactgcatgt tgctctgcgg tttctagatt gatattcatg
       61 attttaaaaa gttcattagt ttataatggc taacagtata tcacagaggt caatattatg
      121 agtcaatttc cacgcattaa acgcttaccc ccttatgtat ttaatacaat aaaccagctt
      181 aagatggaag cgcgggcgcg aggagaggac attatagact ttggcatggg gaatcctgat
      241 caaccaactc cacctcatat tgttaataag cttatagagg ttgctcaaag gcctgatacg
      301 catcgatatt caatgtcgaa aggaattccg agattacgtc gagcgatggc aacttggtat
      361 cttcgacatt atgatgttca tctggacagt gaaaaacaaa tattagctac aataggatct
      421 aaagagggac ttgcacattt ggctttggca atttcaggtc caggtgatac ggtgcttgtt
      481 cctgaccctg cttatcctat ccatacctac ggatttataa ttgcgggtgc taatgtaaag
      541 caaattcctt taatcgatga agttcaattt ttggctgcag tagaagatgc tattaacaga
      601 acctggccaa aacccaaagc cttggttttg aattttccgg caaatccaag tactcactgt
      661 gtcgaatatg agttttttga aaaggtggtt gatcttgcca agcgtaataa catttggatt
      721 attcatgatt tggcctacgc agatatagtt tttgatgggt ataaagcccc ttcaatttta
      781 caagttcctg gtgccataga cattgcaatt gaaacttact caatgtccaa atcttataat
      841 atgcctggtt ggcgtgttgg gtttgcttgt ggtaatgaag agttagtagc tgccttaaca
      901 cgtataaaat cctacttgga ttacggtact tttactccta ttcaggttgc tgcaattaca
      961 gcattggaag gccctgatga ttgtgtgttg gaaataagaa atctttatga aaaacgacgc
     1021 aatcttttat gtgatggact gaatgacatt ggttgggaag tgagcaagcc taaagggacc
     1081 atgtttgttt gggcgcctat tcctttcaat taccgcgcta tggggtctct tgagttcagt
     1141 aaatatttat tgaaagaagc acaagttgct gtttcacctg gtataggttt tggacagcaa
     1201 ggggatggtt atgtccgatt cggcttaatt gaaaataaag acagaataag acaagccttg
     1261 agaaatttaa aagctctgtt taaaagagat ggtttactga aggctattta gtgcaatgga
     1321 tgtcattata aacacagaga gcggctgtga tagcaaagag ggcaaattat taaaatcgca
     1381 aggtttggcg gtacttaatt tattagcatg tggcggttat gaccttgtaa atccccctgt
     1441 aggtaactta ttgaaatcga gccataatct tgagggagat tgggtggttt taactcctat
     1501 gcattggcaa gcatcacata atgatgctgt cattgtcgct cttgataagg atttacgagt
     1561 aacagatgaa gaggtgaaat attggttcga tttgtattct gcctatttag cggaagacgg
     1621 aatgcccctg tatttttatg acaaatacac ttggttattg cgagttgatg ataagccacc
     1681 tcttaatgcc aaaccaattt atcaggtact aaacaagtct ttgatgccag aattatcgca
     1741 actggatgag actatgtact ggcaaaaatt ttttactgaa agtcaaatgt ttttctcttc
     1801 caatgctcgg aaatcattaa ttaatggtgt ttgggcatgg ggtagtggga agttgaaaac
     1861 taaaaaaaca atatcaattt gcacagacaa gcattttctg gcagtagctc aagcatattc
     1921 ttcaaatgtg actttatatg acccgtcagt gaacgtgagc gggtttgatg ttgttctatt
     1981 acatggcatt gattctttaa gcgaattaca tcaagttgaa ataaaaaaaa taccggcaca
     2041 ttggtactgg aacaattgtg ttttaattaa agcaaaatct cattggttta ctcgattatg
     2101 gagaagtcta acgcatgctg attaaacaac gacaacagcc tgagtttact gtcaatttac
     2161 ctaatgtacc tgatgtatta aaacgtattt atataacacg aggcattagt gacgaatctc
     2221 aacttgataa acaattacaa acattacttc cttttcacac tttaaaaggc attaatgaag
     2281 cttgtctaag attagagaat gctttgcgtt cagagcaacg tattcttatt atcggtgatt
     2341 ttgatgccga tggggcaact tctacggcat tagccattac tgccttgcgt gctatgggtg
     2401 ccaagtttgt tgagtacttg gtacctaatc gatttgaatt tggttatggt cttactcctg
     2461 ctattgttga agtggcaagc aaatggcaac ctcatttaat tatcactgtt gataatggta
     2521 tagccagttt tgaaggagta gatgcggcta ataaaatggg aatagatgta ttaattactg
     2581 atcatcattt accagcagaa tcactacctg atgcttgtgc aattgttaat cccaatcagc
     2641 ccggttgttt atttccaagc aagtcaattg cgggagttgg cgttattttt tacgttatgc
     2701 tggcattgcg caggcatctt tccaattgta actggtttaa tgaaatcaac atacctgagc
     2761 ctaatatggc gagttttctg gatttggttg cattagggac tgtagccgac gttgtggggc
     2821 ttgaccaaaa taacaggatc atggttaatc aaggcatgat gcgtattcgt caaggacaat
     2881 gcagagaggg aataaaagcc ttgattgaaa ttgctggtag agattactcc aggctaagag
     2941 agtctgacct tggttttgct atagcgccaa gattaaacgc tgcaggccgt ttagatgata
     3001 tgtcgcttgg tattgaatgt ttgattacga cggatccaaa aatggcaaga aatttctgtc
     3061 aacagttgga tgagttgaat ctggaaagaa ggcaaataga atctgaaatg aaagagcaag
     3121 ccatgcttgc tttggagaag ttgtcaatta acgatgaaga gaataagcac ttaccggttg
     3181 ccttgtgcct tcatgataaa acctggcatc aaggcgtgat aggaatcttg gcaggaagga
     3241 tgaaggaaaa atatcatcgt cctgtcattg catttgcttc tgtcagtgac catgagttaa
     3301 agggctcagc acgttctgtc cccgatttaa acattagaga tgtattggcg gctgttgata
     3361 aagacaatcc aggactgatt cttaaattcg gaggacatgc tatggctgct ggcttaagta
     3421 ttagccccga attattgaat gattttcgct cagcattcgt gtcagaggtt ctgaaacatc
     3481 ttgacttatc acagtgcgaa ggagaaatac tgacggacgg accattgcaa tcccatgaac
     3541 tgagtttgga aacagcacaa ttaatacaac aagctggtcc ttggggccaa gcgtttgcag
     3601 aacccgtatt tgataatata tttgaaattc tggatcaacg aattgtcggt aaaaatcatt
     3661 taaaaatgac attaatttcc tcacaaggag gggagccact ggatgccatt gcttttaatg
     3721 tggatttaaa atcctggcct aatcatagga ttaagtacat tcatgccgcc tataaactgg
     3781 atatcaattt ttatcagggg agaacaaggc tccaattgct catccaggca atgaatcctg
     3841 ttcagcaagc ataaactctt aaattgatgc tatttttgtg tatttctcaa acaacaatgt
     3901 ataatgttat atttgcttta aattgattaa acgtggttaa tagtattttt tccaaattat
     3961 ctatagcgcc aatgattgat tggactaaca gtccatttcg aactctgatg aggttattgg
     4021 ctcctaacgc ccttttgtat actgaaatgc aaacaacagg ggctgtgcaa aataatcctg
     4081 acagagcttt gtattttaat tctgttgaac atcctatcgc tattcaattg ggtggggcag
     4141 ataaatccgc attagttaag tgtgccatta ttgctgaaca gaaaggtttt aatgaaatta
     4201 atttgaattt gggatgtcct agtgacaaag tacaggcggg acgatttgga gcctgcttga
     4261 tgaaggagcc aggacatgtt gctgattgta ttcgtgcaat aaaacaggta gtaggaatac
     4321 cagttacagc gaaaacgagg atcggtattg ataaccagga cagttatgag tttttcagat
     4381 ctttttctct tcacctggtt gaagcaggct gtgataagct tattattcat gctcgcaagg
     4441 cttggttaaa tgggttaaat ccaaagcaaa atagaacgat tcctcccgta aattatgatt
     4501 atgtatatga actaaaaaaa gaaattcccc atatctttat tacaatcaat gggaatatat
     4561 taactgttga agaaataaac agtcatttaa aacatgtcga tggagtaatg ttaggacggc
     4621 ttgcttgcga taacccctat caaattgctg aaattcatca agccttatac ccggaattca
     4681 tcaaaattaa acgcagccag ttgttaataa attatctgga ttattggttt gatcaatctc
     4741 gagccaatct ctcgttaagc attttggtta agccactttt taatttagct tttggattac
     4801 cgggagccaa acattggaaa aagaaattga tgaatattat tcaggagaaa aatacttccg
     4861 gttttagtga gttaatccag tatttattaa atttagaagc aataaatcaa gtcgagtttg
     4921 tataagaaat aatagagcaa atgtcaacat ttcatggaat tttatcaaga tttctagtac
     4981 attaacgagt ttattaattg gaacatcctg agggttattg aggccatgct gagtacgtta
     5041 attaagaaaa tgtttggaag ccgaaatgaa cggacattac ggcgaatgga aaagtctgta
     5101 atggctatta atgcgtttga gccgaaaatg caagctttat caaacgaaga gctggctgga
     5161 aaaactcaag aatttaaaga gcgtttcaat aatggtgaaa gtcttgatga gttacttgcg
     5221 gaagcttttg ctacggttag agaagtatca ttgcgcactc tcggtcttag gcattttgac
     5281 gtacaattaa taggcgggat ggtattgcat gagggaaata ttgctgaaat gcgtaccggg
     5341 gaaggtaaaa cattggttgc aaccttgcca gcctatctca atgccattag cggacgtggt
     5401 gtacatatag tcactgttaa tgattatctt gcgaaaagag atagccaatg gatgaagccc
     5461 atatatgaat ttttagggct tacagttggc gttatctatc ctgatatgtc gcacaaggaa
     5521 aaacaggaag cttacaaagc ggatattgtt tacggaacta ataacgaata cggttttgat
     5581 tatttacgtg acaatatggc ttttagtctg actgataagg tacaaagaga gcttaatttt
     5641 gctattgtcg acgaagtaga ttccatcttg atcgatgagg cgagaactcc attgattata
     5701 tctggcgcgg cagaagatag ttcagagctg tatatcaaaa ttaattcttt gatcccacag
     5761 ttaaaaaaac aagaagaaga gggtgacgaa ggcgattata ctatagatga aaagcaaaaa
     5821 caggctcatc tgactgatgc aggccatctg catatagaag aactgctgac caaagccaaa
     5881 ttattagatc ctggtgagag tctttatcat gccagcaata tcatgttgat gcatcatgtg
     5941 aatgcggcac tcaaggctca tgcaatgttt cacagagata ttgattatat tgtcaaagac
     6001 aatcaagtgg tgatagtgga tgagcatacc ggccgtacca tgcctggaag gcgttggtca
     6061 gaaggtttgc accaggcagt cgaagccaag gagggagtcc caattcaaaa tgaaaatcag
     6121 actctggctt ctataacttt tcagaatttt ttccgcatgt ataataaatt gtcaggaatg
     6181 acgggtactg ctgacacaga agcctatgag ttccaacaaa tatataatct tgaagtggtt
     6241 gtaataccta ccaataggtc aatgattaga aaagacgagg ctgatttggt ttatctaaca
     6301 caggcagata aattccaagc gataatcgaa gatgtacgag aatgtggtgt tcgtagacaa
     6361 cctgtcttgg ttggaacagt ttcaatcgaa gcttctgagt ttttaagtca actcttaaaa
     6421 aaggagaaca taaagcatca ggtattgaat gcaaaattcc atgaaaaaga agcacaaatt
     6481 attgctgaag ccgggcgccc tggtgctgta accattgcga ctaacatggc tggacgagga
     6541 acggatattg ttttaggtgg tagcttggca gctgatctgg ctaatttgcc agctgatgcg
     6601 agtgagcaag aaaaagaagt agttaaaaaa gaatggcaaa aacgtcatga tgaagtgatt
     6661 gcagcaggcg gacttcgaat tattggctct gagcgtcacg aatcaagacg tatcgataat
     6721 caattaagag ggcgagccgg tcgccagggt gatcccggaa gtagccgttt ttatttgtca
     6781 cttgaagaca atttaatgag aatatttgct tctgagcgcg tggcctctat gatgcgtcgt
     6841 ttaggcatgc agccaggtga gccgattgaa catagtttgg ttaccagagc aattgaaaat
     6901 gctcaacgca aactggaagg acatcatttt gacgtaagaa agcaattgtt ggattatgat
     6961 aatgtagcga atgatcagcg gcaggtcatt tatactcagc gttcctctat tatggcaatg
     7021 acagatacac aggaagttgt tgaaatgatg agagaagagg taatggatag tctggttgat
     7081 acttatatcc cccctcaaag tctggaggat cagtgggatc cacaggcttt gtctgatgtt
     7141 ttgtcagatg aatttaaaat caaagcccct gtgcctgact ggattgataa agatcatagt
     7201 atacaacctg agcaaattaa agaaaaaatt cttgctttag ctatagagca ttatgatgaa
     7261 aaagtaagaa aagttggaag gccagtcatt tcacaatttg aaaaatcaat tattttacaa
     7321 actttagata atcattggcg tgaacacttg gctgcaatgg atcaattacg tcagggaatt
     7381 catttaaggg gttatgctca gaaggatcca aagcaggaat acaaaaaaga agcttttagt
     7441 ttgtttacaa tgatgctgga taatcttaag tatgaggtga tcaggatact atcatccgtt
     7501 gaaattcaaa cggaggagga tgcccatgtc gttgaagagc aacgtagagc ggatcagatt
     7561 aaaaaaatga atctcatgca cgaaaattta tcagagaatg atgaagcaag tgaaacgcaa
     7621 acttttagac gacaagaaaa gaaaattggc cgtaatgatc cttgcccctg tggctcaggt
     7681 aaaaaatata aagcctgtca tggaagcctc gtgtgaaagt agcagttgct atcattatcg
     7741 atgaaaaaca gcgtatacta attacccaac gtccttttca tgtcgcacat gggggatttt
     7801 gggagtttcc tggcggaaaa ttggagcctc atgaatctcc agaggatgct ttggttcgag
     7861 agattaggga ggagttaggt gtaattgtaa atgaatatcg ttttctgggg tatgtcgatt
     7921 atgattatcc agataaacac atacaattaa ttatttttat ggtgactcgt tttacaggca
     7981 accctttatg tcaggaagga cagctctata tgaagtgggt aaaaaaagag gaattaaata
     8041 ttaatgattt ccctaaagca aatcatgcgg ttttcgattt aattgatatc cctgaacttc
     8101 aatatcaagc tttataaatg tgcgaataat ctaaagtttg aagctaggac atcatattta
     8161 attgctatgc acaaaataaa ctccctgttt agatgatatt ataacagatc agttgtcgag
     8221 ataaaatcaa tcagaatggt cactacaagg tatgttcaca tagactagag attgcaaaga
     8281 tagtagtctt ttgtaaatga ttagattttg tatttcagtt aattgttaat caattggaca
     8341 gaaaaatgat tactaagcaa aatggaattt atacacttac cctttcttta ttactattct
     8401 cacttcaaat caaagctgct acagattatc aaacatatga aacagtttgt ggtgaaccaa
     8461 cagatatgct ttcttttctt gaaagaggtg gtattgcaac taatccttgt gtagtccctc
     8521 ctagaagcgt attaattagc tctggatatc aatatcagca attaattggg gaaggtgtac
     8581 agcataattt tcctgctgct gcaatacagt taggtttgcc tggttatttt gaggtagact
     8641 tgttgttacc caattatatt aatcagacgg ttgatccacg cataggtttt agtcaaactc
     8701 agcttattgt caatcatgtt ctatggttta acaataagtg ggttattact gctagcggaa
     8761 cgtttgtatt tccatcaggc agcgcttctt ttggcagtcc aaatccagga ggtggtgtaa
     8821 tagggatttt aagttataac tttaattccc aattaaatct tacaggtaat ttgggcatta
     8881 cttctcaatc ggagcctagt tacgatggtg gacaaagtta tacgagtgtt aatccagatc
     8941 ttattttatc gtggactaaa aataaaatat cattatttgc tgagatttat gggcaaagta
     9001 aaacagcacc tgatgaaggg agcggtttta gtactgatgc aggagttctt tatcaagtta
     9061 aaaagaatat tgcaattgat tttgaggttg atcaacgtat cactggcctt ttgaatggtg
     9121 ttgagcgtta ttatggcgga ggaattacta tacaatttaa ttaatgcgga ctcattttaa
     9181 cgcaaagcca gcatagcgcc agggggtcta tatttggcaa aatgcaattt ccatttctat
     9241 tgttttatct ctgatttctt gaccagtgac aagctcataa agcctgatag ttaaactatg
     9301 atgccctaac tggattttgg gtatcatttt caatttctta tctattctta acaagatcag
     9361 atggttaagt gatttaggtg aaagactatg ctgatagaat ccattatttg ctctaatggg
     9421 agtatactga gtaacacctc gtaacaatga caaataaatt tttacagtat cttccagatc
     9481 cttcaggcat gatacccact gactaattgt tttttgcctc attaggggat ctgaatcaaa
     9541 ccacaatacc aaatgagggg aattaaattc gcattcttta gtattggggt ggtgtatttg
     9601 tcttaaagtt tttaaaaatt catcatcatg gattttgtta ctaaatcgac ctggtacatg
     9661 atttaacaca tgaatttgtg tttccagatc atcaaataac tcactatgat tcaatagatt
     9721 agctttttta agtacatgct caattctaat taactctttg ataaatctgc tttttaattc
     9781 cggcttttca actatttcag caatctcaat tatattcttt aatgcaaatc gatgaataac
     9841 ctcatgagac tcattacaag cttcattaat ggttttaaaa aggtactcca ggcgcaaggc
     9901 aattcttgat aaaaaatgag ttgctagttg aaacgttatc atatggtcat gcatagatta
     9961 aggccatcct tagctttatc ctataataat ggcaatttaa atttttatag atgcacattg
    10021 ggtaaaaatg ggctcctggg tttatttaaa aacaatattg ttttttataa acaaatctaa
    10081 aaaatcaagc tattaaggtg aattttgtgt taagactaac ataaaagttg tcgaaaataa
    10141 aaaaatcttt aaaatccata tattaactga tcttttgcta gaaatttgtt gttactgttt
    10201 tatttttgct tccctaagat atttttgatg aagcttattc accttagctt ttaattcact
    10261 caatccagat tcattgataa gcacatcatc cgctgcttcc aggcgctgct ctaaattagg
    10321 ttgtgtggct aaaatagcta atgcttgttt ttttgtgcaa tgatctcttt taacaattct
    10381 gtctagctga gattccaggg gagcaataac caatagaact ttttgaagat aggggtagtg
    10441 atgtttatta aacaacaaag gaatttcaat aaggcaatag ggcgaagtgc agacgatgag
    10501 ttgctcttca atttttttac ggatgactgg atgtaataga ctttcaagcc ataagcgctc
    10561 atttgaattg gaaaatataa tatctcttat acgcttccga tcaagctcac cattattaag
    10621 aaccactgaa gaaccaaaat gtgaaataat atcctggtag cagggtgtat ctttagaagt
    10681 taattctttg gcaattttat ctgcataaat aacatttata cccagctcgg aaaaaaattc
    10741 tgcgactgta cttttgccac tagcaatatt accagttagt cccactgaat aaaccatctg
    10801 agtgggctcc ttcatttttg tttgcacaag ttaccatatt aatataacaa gtagcaggta
    10861 gtgtactgtt ttctttacaa taggcctggg cagctaaggc ggcatcatcc cgatttgagt
    10921 aaaaattact ttcccaaggt tgggcagtaa tatcgatagc tgtgcagcgc cacattggtc
    10981 tggtgcttac tccatttata aatccttcac aatttgatat ggatgtctta catgttgcgg
    11041 gaagttggct ttcttttttg caagcagcaa atgcaacatt gatagccacc tttcggtatg
    11101 aactttgcgc cgtccatgct ttattggctt tatcataagt tacgcatttc caataacttc
    11161 cttttttcat cattggggca gtaaagcctt gatgggataa accaattaat agtagtaaac
    11221 cataaaaaat ataattcctc atattttttc tcctttaagc aagactgtta attcagcaat
    11281 ggatagccct ttggaataat accagccttg ttgtaaacga acaccattat ttgccaggta
    11341 attagcttgg tcttttgttt ccacgccttc tgctatagta actaagttta atccttttgc
    11401 catctggata atggcgtcat ttaatgattc gataatatca ttagaaccaa ttgcttgaac
    11461 aaataattta tcaattttta aataattgaa tggaaaatgt tgcaaataac taatgctggc
    11521 atgaccagtc ccataatcat caacagcaag tgaaaaacct gcttgcctta gttcttgcat
    11581 tttattgata aaagtggtgt cgtttttgtc gaggagctct ctttcagtaa tttcaaagat
    11641 tatttgatga ggtgaaatat tatatttttc tattagctta tggaattgat tgaaaaatac
    11701 tgaatctgtg aaatgtagtg cagaaatgtt aaatgcaagg tggaagtaag aacgttcttc
    11761 taatagagat ctaaattctt tgaaggcaat ttctatgatt tgaagtgtaa ttggcacaat
    11821 taacccggta gcttctgctt cagcaataaa aaaatcaggc ataatgattt gactgtcttc
    11881 attttcccat cgcaataata cttcaacccc tgtgaatcgt tcttcatcac aatcaaacaa
    11941 aggttgatat accggataaa attcctcatt tttaatggcc agttttattg caccttgcaa
    12001 tgaataacgt ttggtcatca tattttttat aagggcatac aaaataaagg aacagatcaa
    12061 aacaataaaa atggttattg tttgaccata ccaaaagtta tgacggatgg ttttattgtt
    12121 ttcagaaaca acgacagaaa caccatttat gctttgtagt ttttctatgg caacgagacc
    12181 taatggggta tattgtgtgt cagtttcttg cgtattgctt aatacccagt ttttattttg
    12241 ttcagtatat cgaatacgaa agatattttt tttctcatat tcattatata gggctataga
    12301 agtggattga tttttatctg tttgtaattc actttttaag acagaagcaa taactaaaat
    12361 acctataagg taattgccca ttttttgctg gatcagataa acgggctggt caaataatgg
    12421 taaattatag gggcctataa tagaacgtga acgaatcggt gttgaaataa gatctttatt
    12481 attgggtaaa gttgaacaaa atagattgtg cttgttgtca ctaataataa caccagagat
    12541 tttaggatta ttcagagtga tatgttccaa gaatggatat aaatctgatt tacaatcgac
    12601 aattttttta ccatatacag gcaaagcata gatttcctga agtagatctt cgataaaacc
    12661 atctatgttg ttactgagat tgattgcaac ttcttttaaa tctttataag ctcttttttg
    12721 gttaatattc caattagcat aaaaagctga aatcaggata ccaatagtta agagaatcca
    12781 tatgattgta aatttcttaa aaagaaataa agataatttg tgaataacct tactcatatc
    12841 ctt
//
LOCUS       DBNCWK010000092        12769 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.092, whole genome shotgun sequence.
ACCESSION   DBNCWK010000092 DBNCWK010000000
VERSION     DBNCWK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.092"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..301)
                     /locus_tag="AC06IO_002093"
     CDS             complement(<1..301)
                     /locus_tag="AC06IO_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HIO9875269.1"
                     /translation="MTNSFSKPVSWIFLFTAVIYLVSVSFIQYPVTTVLKPIPIACLI
                     FGVFRNSLLLSAKVFLILALVFSFAGDVVLTLPISLQLELGIGCFLLAHCFYITLF"
     gene            complement(371..871)
                     /locus_tag="AC06IO_002094"
     CDS             complement(371..871)
                     /locus_tag="AC06IO_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875270.1"
                     /translation="MAKEKYESGNSPSHSEEHGTLGPDVYENARKLNIQLATRGPAIL
                     YALGKASEQVTNGNVVTAVGGMVVGAIGFFGNRFYRNKGDDSAYTYMGQIVGAVQFTA
                     GFTLLALQAFEAFTDTGETTPLLNVVKLSGVALTLTESALILINSYLYGDGSVKTTPI
                     SSPKID"
     gene            1364..2224
                     /locus_tag="AC06IO_002095"
     CDS             1364..2224
                     /locus_tag="AC06IO_002095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIO9875271.1"
                     /translation="MLKRLLGVASLFFILNHQCFAEEHEIAITIDDLPFVGSGTSTPG
                     NLKRTQERFMAIVNTLVDNQVPATGFAIGGAIAKNEWELLEIFRNQGFSIGNHTYKHR
                     SLNSMTAENYIADIEKADTVLSPVMTEPKYFRYPYLAEGSGEKKQKVHEWLAAHQYTI
                     APVTIDSKDYEFNAQFYRIPYRQRPQRLAQFKKRYLAFIWQQTLRAEKKVKKVEGQPV
                     KHILLIHANLINSLCLADIIEMYRSNGYKFITLQEALKGNTATPINDSSPTEALKSET
                     EKKEINEPQS"
     gene            complement(2342..3538)
                     /gene="mltA"
                     /locus_tag="AC06IO_002096"
     CDS             complement(2342..3538)
                     /gene="mltA"
                     /locus_tag="AC06IO_002096"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIO9875272.1"
                     /translation="MKSKLIYLTVALAAIILSAAVWWWLQSPKPTFRQVSFKHLPGWE
                     NAQLKKSLETFQTSCRAFLKQNPERVVGTEQIDLQAKDWHPACRAALKINPVDEQSSK
                     VFFEKWFAPVEFYDDKPVKGLFTGYYMPLIKGSYTKSEEFSIPIYELPSNLVTVDLGL
                     FLPNLKNKKIIGRIAGNKVVPFYTREEINKGAIKNTARVLVWIHSPVDRLFLEIQGSG
                     IIELEDGKRIFIGYDGQNGAPYTAIAGVLIKKGIMTKHNASMQRIKKYLEAHPKQMDK
                     IINQNKSFVFFRKLSLEAALGSQGVALTPGYSLAIDKQWIPMGAPLWLNTTRPDSKNP
                     DVNKPMQRLMIAQDTGGAIRGKVRGDVFWGGGDKATLIAGHMKNEGHYWLLLPAHALS
                     RLAQLK"
     gene            complement(3648..4205)
                     /locus_tag="AC06IO_002097"
     CDS             complement(3648..4205)
                     /locus_tag="AC06IO_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875273.1"
                     /translation="MKKTDSGFILLMTLCITFLMSLLVLASLQLIFLQYKALNSQETF
                     HQNFYQLEHWMMRLARSPLLYAGMDCYMQKDYGANKIANELVNNKGCLLILNQKKYRY
                     FIEDLNEFSCLVLNKDGQKYASHHFRFTVLQDEEHGESAIMQLRFIKLGSNLSSCPEE
                     EIQVKEGVSSWRYLPDYKNFEILTG"
     gene            complement(4202..5008)
                     /locus_tag="AC06IO_002098"
     CDS             complement(4202..5008)
                     /locus_tag="AC06IO_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIO9875274.1"
                     /translation="MLHGLKKASPLFAPMSWMLIEMGRQTGFSLSEMLISLFLASIIT
                     ITLTQFYIRSKSQYLKAQEQLEINFDLLWIGDLLSDSIRKAGFTPCVSIEKLKTFDSR
                     HAERKIINFQIENMPQVQLRINRMSDVYTEVANFMSPTQIVIEESVISNIKHPLIISD
                     CRQGEVHTVSKIDKLSNGYQITLKKPLQFRYEEIAYLGEWLEETWFTKQNEKGEKTLH
                     YKLFQTEELSPFIHEFYVTRRLIKERSVLDFSIGSDKETLRKISVSVRNL"
     gene            complement(4947..5261)
                     /locus_tag="AC06IO_002099"
     CDS             complement(4947..5261)
                     /locus_tag="AC06IO_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIO9875275.1"
                     /translation="MSKHQGFTLMEVLVSLMLMTTVVLMLVEQQLKTRDIINHLIWRI
                     QGSQYLDQVDEAFYISENRLPLPPSNYHFDIRQSQKKIILNVTWFKKSQSIIRSYELD
                     AN"
     gene            5421..6530
                     /gene="hisC"
                     /locus_tag="AC06IO_002100"
     CDS             5421..6530
                     /gene="hisC"
                     /locus_tag="AC06IO_002100"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIO9875276.1"
                     /translation="MSIDFQQLPHAGIRSLIPYVPGKSIEELAKEKGITDIIKLASNE
                     NPLGCSPLALSAIQTMSSHYIATYPSPWNHPLMSKLASYLKVKPEQLFLSNGSDYLFN
                     ILLNCFALHTDRHILTHDYAFSTYAIQANSLQIPINSVPIGHNWEVNITDIVNACNQQ
                     TGIIFIANPNNPTGVLIQQEEIKYLLEQIPKSTLLVLDEAYYEFAASQLTVNSLDWLE
                     EHPNLVVTRTFSKIYGMAGLRLGYAIANPSIINILKRVQLPFIVNQVALAAAYAAIDD
                     DDFIQSSLKMNNEGMSQLQAGFNELNIKYLPSSCNFLTFDCEEDSMALYNYLLDNGII
                     VRPLHAYRMNNFIRVTIGTKEQNSRFLTALKNFYL"
     gene            6567..6908
                     /locus_tag="AC06IO_002101"
     CDS             6567..6908
                     /locus_tag="AC06IO_002101"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIO9875277.1"
                     /translation="MTSDLSSKHCESCEGIGAALNSEQIKNLLPQLNTKWEVTEDNRI
                     IKRAFSFKNFYETMAFVNAIAWIANIENHHPDLEVGYNYCHVHFMTHALNGLTHNDFI
                     CAAKIDKLLVD"
     gene            complement(7098..8015)
                     /gene="secF"
                     /locus_tag="AC06IO_002102"
     CDS             complement(7098..8015)
                     /gene="secF"
                     /locus_tag="AC06IO_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIO9875278.1"
                     /translation="MEFFNPNSKIDFMGARRWTALFSILIFVVSIGALMINGLKWGLD
                     FTGGTQIEISYSKAADLPLIRENLNKAGFKEAQVVSYGTSKDVLISIAPRADKQQSML
                     VDQVMEVLPGAVKQRVDFVGPQVGQELATKGALAIIVSLLATMIYIAMRFEYKLAVSS
                     AVALIHDPVLILGVFALFGIEFDLKALAGLLAVIGYSLNDTIVVFDRVRENFVKIRRT
                     SPIDIMNISINQTLSRTIMTSVLTLFVVVALFVYGGEAIYGFSLALIIGIVIGTYSSI
                     YVAGALAVAMGLDNKDFLPSHRREVDDRP"
     gene            complement(8028..9884)
                     /gene="secD"
                     /locus_tag="AC06IO_002103"
     CDS             complement(8028..9884)
                     /gene="secD"
                     /locus_tag="AC06IO_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIO9875279.1"
                     /translation="MQNKYPLWKNLMLIVIAVIGFVYAIPNLYTENPVVQISAGTDED
                     TETIKNQVESILKNANMDYKSLVVERDGVEVVFSSTDSQLLARDVIKNSLGSDYTVAL
                     NLAPSTPAWLEAIHADPMKQGLDLRGGVHFLLEVDVESVISRRYEGIMKNIGQELREA
                     GIRYSGIRYLSDKGIELRFRDSDSMENALVELRQKISDVVFVKTKTQNSILGAISPAE
                     LNSIRQNTIEQTMSILRNRVNELGVGEAVVQQQGATRVAVDLPGIQDAARAKQILGGT
                     ATLEFHLVDQENDPFIAKQTGVVPVNDKLYMMDGHPILLKRQVVLSGDSITSAVSSFD
                     QQTASPSVQIQLGGGGESLFTKVTRENIGKRMAIVFVETKTTTQTINGEEKRVTKKEE
                     RVISAPVIQNALGSNFQITGLTDSKEASNLALLLRAGALPAAIYPVEERTVGPTLGKE
                     NIRRGLISLEVGMGLILVLMLVYYHFFGLIADIALMLNLVFLCALLSIIDATLTLPGI
                     AGIVLTVGMAVDANVLIYERIREELRNGLSPQAAIHAGYERAFSTILDANVTTLIVAI
                     ILFAVGTGPVRGFAVVLSLGLLTSMLTGITYTRAIVNWYYGGRNVKKLSIGI"
     gene            complement(9906..10241)
                     /gene="yajC"
                     /locus_tag="AC06IO_002104"
     CDS             complement(9906..10241)
                     /gene="yajC"
                     /locus_tag="AC06IO_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIO9875280.1"
                     /translation="MSFFISDALAAAGNAPAAQTDGTFSLIMIVAIFVLFYFMIIRPQ
                     SKRAKEHRELINRLKKGDEIVTSGGILAKVVSLDDQYIKVSPAEGIEINLQKGAVTAV
                     LPKGTIKSL"
     gene            complement(10387..11220)
                     /locus_tag="AC06IO_002105"
     CDS             complement(10387..11220)
                     /locus_tag="AC06IO_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuine tRNA-ribosyltransferase"
                     /protein_id="HIO9875281.1"
                     /translation="MLSTNQNFVPVLNTEAGLCLTAANWQEIKITIASCYLDLLLLKP
                     GYSLLKNITDFTKYLGWSGHLILNASRLVSDKNGMVVLISPYDGSRIKLTNAELVHLI
                     LHIKPVAVLLPETLVNGFSELWEQWDDTILPFLSMKQLETLQVPGKHGVYFNFTEAKY
                     NDDFLSQLQKWSEFPLYVSGPIGADLIEDLNRKKSILIESDEPAKNAMRGIVYGREGN
                     VDLTDESQAFNFQLIDENCSCPTCSAQLTRAYLHHLLANTPLLCQRFLIQHNAYYVQG
                     N"
     gene            complement(11267..12280)
                     /gene="queA"
                     /locus_tag="AC06IO_002106"
     CDS             complement(11267..12280)
                     /gene="queA"
                     /locus_tag="AC06IO_002106"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIO9875282.1"
                     /translation="MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFR
                     EIADFLQPGDLLVMNDSKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLK
                     SNDLIYLDAAKRLEVLQRQDDLFLCKASENILDLLNDLGHIPLPPYIDREDESLDKER
                     YQTVYAKCAGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQD
                     HKMHSEWFTVSPDLCTAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIF
                     IYPGYQFKVCDGLITNFHLPESTLVMLVSAFIGHQECMALYQEAIAKRYRFFSYGDAS
                     LLL"
     gene            complement(12396..12767)
                     /locus_tag="AC06IO_002107"
     CDS             complement(12396..12767)
                     /locus_tag="AC06IO_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875283.1"
                     /translation="MVFFSQEFIRTFKKILAIRGAKLLIPLGIASWLIYNFDYLFIWV
                     IYYIREVLQAVLAFLTRIMPFKPYSTSIALIILLTTVSVGPVLLLDLIYRKRTYKGYA
                     YPYLTSTLILIFCTLILLVVS"
BASE COUNT         4107 a         2642 c         2131 g         3889 t
ORIGIN      
        1 aaaataaagt aatataaaaa caatgcgcta acaagaagca accgatacct aattccaact
       61 gcaatgaaat aggtaaagtt aataccacat ccccggcaaa ggaaaataca agtgccaaga
      121 tcaggaaaac tttagccgat aagagaagag aatttctaaa taccccaaag attaaacagg
      181 ctatgggaat aggttttaaa acagtagtaa caggatattg tataaacgaa acactgacta
      241 aataaataac agcagtaaac aaaaatatcc aggacactgg tttggaaaaa gaattagtca
      301 taaatagtcc cttattttta aaatcaaagc cattgatttg agctgtctta tatctcattt
      361 tttattatat tcaatcaatt ttagggcttg atatcggtgt tgttttaact gaaccatccc
      421 catacaaata cgaattgatt aaaattaaag cgctttcggt taatgtcaat gcaaccccac
      481 ttagtttaac cacattgagc aagggagttg tttctccagt atctgtaaag gcctcaaatg
      541 cctgtaatgc taataaggtg aatcctgcag taaattgcac cgcaccaaca atttgtccca
      601 tataagtata agcggaatca tcacccttgt ttcgataaaa gcgattacca aaaaaaccta
      661 tcgctccaac caccattcca ccaaccgcgg ttacaacatt accattagtt acttgctcac
      721 tggctttgcc taatgcatac agtatggcag gccctcttgt tgccaattga atattcagct
      781 ttcttgcatt ttcatagaca tctggaccta aagtgccatg ttcttctgag tgtgatggtg
      841 agtttcctga ttcatatttt tcttttgcca ttatcaactc ctgtttagca aaattccggg
      901 taagacatta tcctttcaat ttctcagatt gtatccacgg ccttgcctta ttatttgacg
      961 taatcgaccc taatttcacg tgaattatgg ataagaacag ccgttatatt gaatcgctca
     1021 cgtgaggaga catttatgcc atctcgaagc cttgtatgga atttaatatt ttatatgctt
     1081 aggctttgag aggatgctta cacatctccc tgacccaaac cgaccctgga tatcgagtgc
     1141 tccttatata acccttatgc ataactcacg ttaatttaag taattctcca ttagtcactt
     1201 taataaatta tccaatagcc gaaaaattga agtatttatt gtacctaaac cctctcaaaa
     1261 tacctactta tttccaagca ggtggaaatt taatttatcg tctatagtta aattaaatgc
     1321 ctaaaataat aagagatttt tcaaattgaa aagaggggaa aatatgttaa aacgcttact
     1381 cggtgtagca tcattatttt tcatattgaa ccatcaatgc tttgccgaag aacatgaaat
     1441 cgccattact attgacgatt taccttttgt aggctctggg acaagcactc cagggaatct
     1501 gaagcgcacc caggaaagat ttatggccat agtgaatact ttggttgaca atcaggttcc
     1561 tgccacaggt tttgccatag gaggggctat agcgaaaaat gaatgggagc ttctggaaat
     1621 ttttcgtaat caaggttttt caattggcaa tcatacctat aagcatcgaa gtttaaattc
     1681 aatgactgca gaaaattata ttgctgatat cgaaaaagcg gataccgttc tatctcctgt
     1741 tatgactgag ccaaaatatt tccgttatcc ttatcttgcc gaaggcagtg gagaaaagaa
     1801 gcaaaaagtg catgagtggc tggcagctca tcaatacacg attgctcctg tcaccataga
     1861 cagcaaggat tatgaattca atgcgcaatt ttatagaata ccctataggc aaagaccaca
     1921 acgtttagca caattcaaaa aacgctatct tgcttttatc tggcaacaga ccctgagagc
     1981 agaaaaaaaa gttaagaaag tagaaggaca acctgttaaa catattttac tcatccatgc
     2041 gaatctgatt aatagcttgt gtttggcgga tatcattgaa atgtatagaa gcaacggtta
     2101 taaattcatt actctgcaag aggctctaaa agggaataca gcgactccaa ttaatgattc
     2161 atcaccaact gaagctttga aatcggaaac tgaaaagaaa gaaattaatg agcctcaatc
     2221 ttaaaaagta tagagttatt tcttgaaaca aatgatttac aatgctatca gggattcatt
     2281 gtcacacgac tggtgaatcc caatagccaa ataaaaaatt gcaaagacag gacttacgcc
     2341 cctacttcaa ctgtgccaag cgcgataatg catgtgccgg cagaagaagc caataatgac
     2401 cctcgttctt catatggcca gcaatcaaag tggctttgtc accgccaccc caaaatacat
     2461 cacctcttac tttaccacga atagccccac cagtgtcttg agctatcatc aaacgttgca
     2521 taggtttatt gacgtcagga tttttactat ctggtcttgt agtgtttaac cacagaggcg
     2581 ctcccattgg tatccattgt ttatcaatag caagagaata accgggtgtt aatgccacac
     2641 cctgtgaacc caaagccgct tctaatgaca acttgcgaaa aaaaacaaat gatttatttt
     2701 gattgattat tttatccatt tgtttgggat gagcttccag atacttttta atcctttgca
     2761 tggaagcatt gtgcttggtc ataataccct ttttaattaa tactccagca atggcagtat
     2821 agggggcacc attttgacca tcatagccta taaaaattcg tttgccatct tcaagctcaa
     2881 tgattcctga tccctgaatt tctaaaaata acctgtctac agggctatgg atccacacca
     2941 aaacacgtgc tgtgttttta attgctcctt tgttaatttc ctctcgagta taaaatggta
     3001 caactttatt acccgcaatt cgtccaataa ttttcttatt ttttaaatta ggaaggaata
     3061 aacccaagtc aacagtcact aagttactgg gtagttcata aataggaata ctaaattcct
     3121 ctgatttggt ataactgcct ttaattaaag gcatataata gcccgtaaat aaacctttta
     3181 ctggtttatc atcatagaat tccacagggg caaaccactt ctcaaaaaat acttttgatg
     3241 attgttcatc aacaggattt atttttaaag cagcgcgaca ggctggatgc caatcttttg
     3301 cctgtaaatc aatctgctct gtgccaacaa cacgttcagg attttgtttt agaaaggctc
     3361 gacaagatgt ttgaaaagtt tctaacgatt ttttaagttg tgcattctcc cacccaggta
     3421 aatgcttaaa gcttacctgc ctgaaagttg gcttgggaga ttgcaaccac caccacactg
     3481 ctgcgcttaa tataattgca gccaaggcta ctgtcagata aattaatttc gatttcatag
     3541 gaggtcaatt tttacacgtg atggagaaaa atgcaactaa aaattattgt tattagtcca
     3601 tcttactaca aaaaacatcc ggttataata gttttttttc aataatttca accagttaat
     3661 atttcaaaat ttttataatc tggcaagtat ctccagctgc ttaccccttc cttcacctgt
     3721 atttcctctt ctggacaaga ggacaaattg ctacccaatt tgataaacct taattgcatg
     3781 atagcagatt cgccatgctc ctcgtcttgt aatacggtga atctaaaatg gtgagaagca
     3841 tatttctgcc catctttatt aagaaccaag caagaaaact cgttaaggtc ctcaataaaa
     3901 tatctgtatt ttttctgatt taatatgagc aaacatcctt tattgttaac caactcgttt
     3961 gctattttat tagctccata atctttttgc atataacaat ccatacctgc atacaataac
     4021 ggtgaacgcg ccagtcgcat catccaatgt tccaactgat agaaattttg atgaaatgtc
     4081 tcctgtgaat tgagtgcttt gtattgcaag aatatcagtt ggaggcttgc caaaaccaat
     4141 aagctcatca agaaagttat acagagcgtc attaaaagaa tgaaaccgga atcggttttt
     4201 ttcataaatt tcgtaccgaa acactgattt tacgtagcgt ttccttatcg ctaccaattg
     4261 aaaaatccaa tactgacctc tctttgataa gacgtctggt aacataaaat tcatgaatga
     4321 atggacttaa ttcttctgtt tgaaacagtt tgtaatgtaa tgtcttttca cccttttcat
     4381 tttgtttagt aaaccatgtt tcttcaagcc actctcccaa ataagcaatt tcttcgtatc
     4441 gaaactgcag tggttttttt aatgtaattt gatatccatt gcttagttta tctatttttg
     4501 aaactgtatg tacttctcct tgccggcaat cagaaataat taatgggtgt tttatatttg
     4561 atattactga ctcttcaatc actatttgtg ttgggctcat aaaattcgcc acttcggtat
     4621 aaacatcact cattctgtta atacgaagtt gaacttgtgg catattttca atttgaaaat
     4681 taattatttt cctctctgca tgtcgagaat caaaagtttt taatttttcg atacttacac
     4741 atggagtaaa tccagccttt ctaatactgt cgcttagtaa atcaccaatc cacagcaaat
     4801 caaaattaat ttctaactgt tcttgtgcct ttaggtattg agacttgctt cttatataaa
     4861 actgggtaag ggttatcgtt ataatacttg ccagaaaaag actaattagc atctcagata
     4921 aactaaatcc tgtttgtctt cccatttcaa ttagcatcca gctcatagga gcgaataatg
     4981 gactggcttt ttttaaacca tgtaacattc aaaatgattt tcttttgaga ttgcctgata
     5041 tcaaaatgat aatttgaagg cggcagaggg agtctattct cactaatata gaatgcttca
     5101 tcgacttgat ctagatattg ggaaccttga attcgccaaa taagatgatt gattatatct
     5161 ctggttttca actgttgttc cactaacatc agaactaccg ttgtcattag cattaatgac
     5221 acaagtactt ccattagagt gaaaccctga tgcttgctca taataaatct cttcctgagg
     5281 ttggactgcc catgaaggtt aactgttcgt ttatataaat tcaatataac gatttataaa
     5341 ttctaaaaaa taaattcatt cgtgacagct ggcatatgct taggtaatat aaaaacattt
     5401 ttctgtctag gaatagagtt atgtctattg attttcaaca attacctcat gccggaatac
     5461 gttctttaat tccttatgta ccaggtaaat ccatcgaaga attggcaaaa gaaaagggaa
     5521 taactgatat tatcaagctg gctagtaatg aaaacccatt gggatgcagc ccattagccc
     5581 tttccgccat tcagactatg tcatcgcatt acatagcaac ctacccatcg ccctggaacc
     5641 atcctttaat gtccaaactt gcaagctact taaaagtaaa acctgagcaa ttgtttctaa
     5701 gcaatgggtc tgattacctg tttaatattt tattaaattg tttcgctctt cataccgaca
     5761 gacatatcct tactcatgat tacgcgttta gcacttatgc catccaagct aattccctgc
     5821 aaataccaat aaatagtgtg cctattggtc acaactggga ggttaatatc actgatatcg
     5881 ttaatgcttg caatcaacaa acaggaatta tttttatagc taacccaaat aatccaactg
     5941 gggtgctaat ccaacaagaa gaaattaaat atttattgga acaaattccg aaaagtactt
     6001 tgttagtttt agatgaagcc tattatgaat ttgctgctag ccaattgact gtcaatagtt
     6061 tggattggct tgaagaacat cctaatttgg ttgttactcg tacgttttca aaaatctatg
     6121 gtatggcagg tttgcgttta ggttatgcta ttgccaaccc atccattatt aatattttaa
     6181 aacgagtaca attgccattt atagtcaatc aagttgctct tgcagcagcc tatgctgcca
     6241 tcgatgacga tgacttcatc cagtctagtc tcaaaatgaa taatgaagga atgtcgcagc
     6301 tacaggcggg ctttaatgaa ttaaacataa aatacttacc ctcttcctgt aatttcctta
     6361 cttttgactg cgaagaggat agtatggctc tttataatta tctattggat aatggcataa
     6421 tagtaagacc tctgcatgct tataggatga ataatttcat cagggttact attgggacca
     6481 aagaacaaaa ttccagattt ctaacagcac tgaaaaattt ttatttgtaa ctctttttaa
     6541 attaaaaata aaattaagga atcaaaatga ctagtgattt aagtagtaag cattgtgaat
     6601 cttgcgaggg aattggcgct gcgctcaact ccgagcaaat caaaaattta ctacctcaat
     6661 taaacacaaa atgggaagta acagaggaca accgaattat caagcgtgct ttttcattta
     6721 aaaattttta tgaaacgatg gcttttgtca atgcgattgc ctggatagcg aatattgaaa
     6781 atcaccaccc tgatttagag gtcggctaca attattgcca tgttcacttt atgacgcatg
     6841 cacttaatgg tttaactcat aatgatttta tttgcgcagc aaaaatagat aagttactgg
     6901 ttgattaaaa attattgact ctttttaaga agatacattt taacaaaatt ttgtaattgt
     6961 ttattatccg ctccttgatg gtcccggctc agattatggc tgactttgaa ttgcgtcgaa
     7021 ttgggcaaag ccgagccgcg actgtcagga agcggataat aagagtataa tacacggatg
     7081 ataataagcc ccccacctca aggtctatca tccacctccc tgcgatgact gggtagaaaa
     7141 tctttgttat ccaagcccat ggctactgct aaagcgccag caacataaat tgaagagtaa
     7201 gtacctatta ctattcctat aatcaaagcc aatgaaaacc catatatcgc ctcaccccca
     7261 tagacaaaaa gcgcaaccac aacaaacagt gtcaacacag atgtcatgat cgttcttgag
     7321 agagtttgat tgattgaaat attcataata tctattggac ttgttcggcg aatttttaca
     7381 aaattttctc gaacacgatc aaagacaaca atggtatcat tcaaggaata gccaataacg
     7441 gccaacaacc cagcaagggc cttcagatca aattcaattc cgaacaaagc aaaaacgccc
     7501 agaatcaata ccggatcatg aatcaaagcc actgctgaac ttacagcgag tttatattca
     7561 aaccgcatag cgatatagat catcgtggct aacaaggaca caataatcgc aagagcccct
     7621 ttcgttgcta attcttgtcc aacttgtgga ccaacaaaat caactcgttg ctttactgct
     7681 ccaggcagta cttccatcac ctgatctacc agcatgctct gctgtttatc cgctctcggt
     7741 gcaatgctga taagtacatc ttttgatgta ccataactaa ccacttgagc ttctttaaaa
     7801 cccgctttat taagattttc tctaattaaa ggtaaatcag ccgctttgga gtaactgatt
     7861 tcaatttgcg ttccaccagt aaaatccaat ccccatttca aaccattaat catcaatgcg
     7921 ccaatagaca ctacaaaaat caatatagaa aacaatgcag tccaacggcg cgcgcccata
     7981 aaatctattt ttgaatttgg attaaaaaat tccatctgag tctcgcctta tatgcctata
     8041 gataattttt tgacattacg cccaccataa taccaattca caatagccct ggtgtaagta
     8101 ataccagtta acatagaagt taacaaacct agagaaagga caaccgcaaa cccgcgcacc
     8161 ggtcctgtgc ccaccgcgaa taaaatgata gctacaatta aagtagtcac gttggcatcc
     8221 aatatagttg aaaatgctcg ctcatatcct gcatgaatag cggcttgagg agacagtcca
     8281 ttgcgcaact cctccctaat ccgctcataa atcaggacat tggcatcaac agccatgccc
     8341 acggtcaaaa caatccccgc aattcccggt aaagtcagag tcgcatcaat tatggataac
     8401 aatgcgcaaa gaaaaactaa attcagcatc aatgcaatat cagcaattaa accaaaaaaa
     8461 tgataataca cgagcattag taccaaaatt aaacccatac caacttccag ggaaataagt
     8521 cctcggcgaa tattttcctt tccaagagtt gggccaacgg tacgctcttc aacaggatag
     8581 attgcagcgg gtaaagcgcc agctcttaat aataaagcca gattacttgc ttctttacta
     8641 tcagtcagtc cggtaatctg gaaattgctg cctaaagcat tttgaatcac gggcgcacta
     8701 ataactcgct cttctttctt tgttactcgt ttttcctcgc cattaattgt ttgtgtagtc
     8761 gttttggttt ccacaaaaac gatcgccatg cgcttaccga tattttcacg cgtaacttta
     8821 gtaaacaagc tctcgccccc accacccaat tgaatttgaa cagatggaga agccgtttgt
     8881 tgatcaaaac tcgatactgc tgacgtaata gagtcaccgc ttaaaacgac ctgtctcttt
     8941 aataagatag gatgaccatc catcatatac agcttatcat tgacaggaac tacgccggtt
     9001 tgctttgcaa tgaaagggtc attttcctga tcaaccaaat gaaattccaa agtagcagta
     9061 ccaccaagta tttgttttgc tctggccgcg tcttgaatgc ctggcaaatc aacggcgact
     9121 cgagtcgccc cttgttgttg tacgacagct tcaccaactc cgagctcgtt gacacgattt
     9181 ctcaagatac tcattgtctg ctcaatagta ttttgtctta tggaatttaa ttctgctgga
     9241 gaaatggcac ctaaaattga attttgtgtt ttagttttaa caaaaaccac atcagatatt
     9301 ttttgtctta actcaactaa agcattttcc attgagtcag aatctctgaa acgcaattct
     9361 atccctttat ctgataaata acgtatgcct gaataacgaa tacctgcttc acgtaattct
     9421 tggccaatat ttttcattat tccttcataa cgccgactta taacactctc gacatccact
     9481 tcaagaagaa aatgaacacc gcctcttaag tccaaccctt gcttcatggg gtcagcatga
     9541 attgcttcaa gccaagctgg tgtggaaggc gctaaattca aagccactgt gtaatcagat
     9601 cccaggctat tcttgataac atcacgagct aataattgag aatcagtaga agaaaatact
     9661 acctctaccc catccctctc cacaaccaga gatttataat ccatattggc attttttaat
     9721 atggactcta cctgattttt tattgtctct gtgtcttcat ctgtacccgc agaaatttga
     9781 acaacaggat tttccgtata caaattcggt atagcgtaaa caaatccaat gactgcaatc
     9841 actatcagca ttagattttt ccatagagga tatttatttt gcatttctga ttccctatgc
     9901 ctaacttaaa gtgatttgat tgtgcctttc ggtaatactg cagtaactgc gcctttttgc
     9961 aaattaattt ctataccttc tgctgggctt actttgatgt attgatcatc caggctcaca
    10021 acttttgcca aaatccctcc ggatgttact atttcatccc ccttttttaa gcgatttatc
    10081 aattctctat gctctttggc tcttttgctt tgcggcctta ttatcataaa ataaaacaag
    10141 acaaaaatcg caacgatcat aatcagtgaa aaagttccat cagtttgagc agctggcgca
    10201 tttcctgctg cagctaatgc atcactaata aaaaaactca taaaatctcc tcaaatcggg
    10261 tattaatttc ctggttttct ttcgaagatg gtcgataaac caatttcaaa agatagttct
    10321 aagaactctt gtatggatta ccagcgtgac atcatatagc gaatttactc gaaggtaaat
    10381 gaatagttaa ttaccttgaa cgtaatacgc attatgttga atcaagaatc tctggcatag
    10441 caatggggta ttagctaaca aatgatgcag ataagctctt gtcaactgtg ctgaacaagt
    10501 aggacatgag caattctcat caatcagttg aaaattgaaa gcctggcttt catccgttaa
    10561 atcaacattc ccttcgcggc cgtatacaat gccccgcata gcattttttg caggctcatc
    10621 cgattcgatt aaaatcgatt tcttcctgtt taaatcctcg attaaatcag cgccgatagg
    10681 accagagaca taaagaggaa attcagacca tttttgcaat tgactcaaga aatcatcatt
    10741 atattttgct tcagtgaaat tgaaatacac accatgtttt ccaggcacct gcaaggtttc
    10801 aagttgcttc attgacaaaa aaggtaatat agtatcatcc cattgctccc atagctcgga
    10861 aaaaccattc acaagcgttt caggtaaaag cactgcgaca ggcttaatat gcaaaatcaa
    10921 atgcactaac tctgcgttag ttaatttgat tcgacttccg tcataaggtg agatcaatac
    10981 caccatcccg tttttatcgg atacaaggcg gcttgcattg aggatgagat gacctgacca
    11041 ccctagatat ttggtgaaat cagtaatatt tttcaataat gaatatcctg gctttaataa
    11101 caataaatcc agataacaag atgcaatggt aatcttaatt tcttgccaat tggccgcagt
    11161 aagacatagt cctgcttcag tatttaaaac aggaacaaaa ttttgatttg tactcaacat
    11221 ccactatcct ctaactgtag ccaaaattat tatattccag agagctttat aacaataaac
    11281 tggcatctcc ataactaaaa aaacgatacc ttttagcgat tgcttcttga taaagagcca
    11341 tacactcctg gtgcccaata aaagcagaca ccaacatcac cagagttgat tcaggcaaat
    11401 gaaaattagt tattaatcca tcacaaactt taaactggta gccaggataa atgaaaatat
    11461 cggtatcacg actacatgga attagctctc ctcccatagc agcgctttca aggctgcgca
    11521 atgcagtagt cccaacagca attactctat ttcccataga tttggcagcc ttcactgcag
    11581 tgcacagatc aggactaact gtaaaccact cactatgcat tttatgatcc tgaatgcgtt
    11641 cacagcgaac tggcctgaat gttcctgccc ctacatggag agtgacataa gcaatattaa
    11701 ctcctcgcgc tctaatactg gataaaacag catcatcaaa atgcaatcct gctgtaggcg
    11761 cagcaacaga ccccgcgcac ttggcataga cggtttgata tctctcctta tccagtgatt
    11821 catcttctct gtcgatataa ggtggtaaag gaatatgccc gagatcattt aaaagatcta
    11881 aaatattttc gctcgcctta caaagaaata aatcatcctg cctttgtaat acttcaagcc
    11941 gcttagcggc atcaagataa atgagatcgt ttgattttaa tgatttgctc gctttgatat
    12001 gggcaaggaa agtaaaatcg ccagttatcc tctctactaa cagttcaacc ttgccgccag
    12061 tggctttgtg gccgtataag cgtgcaggta tcactttgct gtcattcatt accaacaagt
    12121 cccctggctg taagaaatct gcaatttccc gaaattgata atggccatat tcttctgtct
    12181 gtcgattata aattaaaagc cgtgaatcac ttctattcgc cagaggatat tgggctatta
    12241 attcggaagg caaatcaaaa taaaaatctt gcttattcat agaacaacct aaaaacaaat
    12301 tggtgcaatt atacaccgaa ctttagtctt aaaatcaatt atcaggaatt tattcatttt
    12361 attagaaaat taatatcatt tgttttttta tctgtttaag agactacgag caagataagc
    12421 gtacagaaaa ttaaaatcag ggtgcttgtt aaatagggat aagcataccc cttgtaagtt
    12481 cgtttgcgat agatcaagtc cagtaataac acaggtccca ctgaaacagt agtaagtagt
    12541 ataataagtg ctatcgatgt ggaatatggc ttaaaaggca taatccgcgt caaaaaagct
    12601 aacacagctt gcaatacttc tcgaatataa taaattaccc aaataaataa ataatcaaaa
    12661 ttataaatta gccaggaagc gatgcctaag ggaatgagta acttagcccc tctgattgcc
    12721 aaaatttttt taaaggttct aatgaattct tgagagaaga aaaccataa
//
LOCUS       DBNCWK010000093        12449 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.093, whole genome shotgun sequence.
ACCESSION   DBNCWK010000093 DBNCWK010000000
VERSION     DBNCWK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.093"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1139
                     /locus_tag="AC06IO_002108"
     CDS             <1..1139
                     /locus_tag="AC06IO_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9875284.1"
                     /translation="NYLDKFNFQVWTNGGKLLLHSPTAPKIPLTAETDGFSDKRISNQ
                     DWRVFTTYNDKAGIRTVLAERYDTRNELGHRIAQDDLYIMLLTFPLSGLLIWIIIGRG
                     LDSLDRVAEEVANRAPSHLEPVDLQEVPEEIKPVIDELNKLFFRLKEGFEREKRFAAD
                     AAHELRTPLAALKAQAQVALNSNDIEEKNQALQKLIASVNRSTHIVQQLLTMSKLVPE
                     ATELHEQDEVNLSKLTREILAMLAPSAVEKQIELEFETDEKMPKIYGNPTALGILIRN
                     LVDNSIRYSMENGRVIVRLLKHNNEIILEVCDNGPGIPPELQKRVFERFFRVLGNKSP
                     GSGLGLAIVQQICELHGGRVVLDSPKIGTGLIVRVYLPIKTKPK"
     gene            complement(1180..1500)
                     /locus_tag="AC06IO_002109"
     CDS             complement(1180..1500)
                     /locus_tag="AC06IO_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875285.1"
                     /translation="MKRMIGFMVSVMLTFAVNAADSQVNTHKHQTDKGKVTPGMSIER
                     NTEQSTAITADAASSNTTEKVTSQQMKEHKKSVKEANYNAQKKEEEAKTATDTQKHEA
                     DSPS"
     gene            complement(1550..2149)
                     /gene="ruvA"
                     /locus_tag="AC06IO_002110"
     CDS             complement(1550..2149)
                     /gene="ruvA"
                     /locus_tag="AC06IO_002110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIO9875286.1"
                     /translation="MIGWLHGQIIDKHQPGKLVLDVNGVGYDVETSLNTFFQIENGNQ
                     PIGLHIHTIVREDALLLYGFLDKEERSLFRSLIKVNGVGPKLAMTVLSSISPKEFIQC
                     IHQENAALLTKLPGIGKKTAERLVVEMRDSIKQFDGSVSDTFQKQAGSTHSQQEAISA
                     LEALGYKPQEAWKVVNKIDNGNKSCEQLIREALQILSSR"
     gene            complement(2146..2670)
                     /gene="ruvC"
                     /locus_tag="AC06IO_002111"
     CDS             complement(2146..2670)
                     /gene="ruvC"
                     /locus_tag="AC06IO_002111"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIO9875287.1"
                     /translation="MTIILGIDPGSRVTGYGLIKESGRKIAYIDSGCIRTSNDVELSH
                     KLLQIYDGICELMDHYSPTEVAIEQIFMHNNPNSALKLGHARGVAMVAAASHRVKICE
                     YSAREIKQSVVGYGAAEKDQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSK
                     LIGSRGTKRRRMRL"
     gene            complement(2670..3413)
                     /locus_tag="AC06IO_002112"
     CDS             complement(2670..3413)
                     /locus_tag="AC06IO_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIO9875288.1"
                     /translation="MAGHSKWANIKFRKGVQDAKRGKIFTKLIREITVAARMGGGDES
                     SNPRLRDAVKKALNANMKRDTIDNAVKRGVGGADGEAMIAMRYEGYGPGGVAILVDCL
                     SDNKNRTVSEVRHAFSKHGGNLGTDGSVSYLFTNQGEILMASNQPEDKVMEIAIDAGA
                     SDVAVEDSQIEIITPVEAYHTVLNALQDAGLEVEQSHLTMRAQTLVPISDETAESLIK
                     LIDMLEDLDDVQEVYSNAEFSEKILESMN"
     gene            3560..4810
                     /gene="hmgA"
                     /locus_tag="AC06IO_002113"
     CDS             3560..4810
                     /gene="hmgA"
                     /locus_tag="AC06IO_002113"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIO9875289.1"
                     /translation="MYLQGFGNYHHSEAVKGALPPNQNSPQHCSLGLYAEQLSGTSFT
                     RPRHNNLRSWLYRILPTVTQGTYYPYEFNIMQPFVDELSPNAMRWSPLYNSSQIKCDF
                     VEGLFHIAGSPLVNAYTYYCNHSMSDKYFANNDGELLFVPYTGEIHLHTEFGKLMLSS
                     GSIAVIPRGVKFKVEVISKEAKGYLCENSGNPLTLPQLGPIGANGLANPRHFQYPVAA
                     FENSGGEHTIICKNQKKLWFTVCNHSPLNVVAWHGNYAPYCYDLSLFNTINTVSFDHP
                     DPSIFTVLTSESEIPGVSNLDFVIFPPRWMVAEHTFRPPYFHRNYMNELMGLVYGEYD
                     AKKEGFIPGGISIHNCMTPHGPDYESYEIAASQDLKPNYINSLAFMFETKDYWQVTEQ
                     AYLHPSRQMDYLNCWQGFKLEFSQ"
     gene            complement(4909..5934)
                     /gene="rpoS"
                     /locus_tag="AC06IO_002114"
     CDS             complement(4909..5934)
                     /gene="rpoS"
                     /locus_tag="AC06IO_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HIO9875290.1"
                     /translation="MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDIEPDIEKMEEELD
                     ELPEFTDDEAFPSFQRGKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDME
                     ARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYAT
                     WWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKP
                     LEDVQKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLL
                     DKLTENQQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQVEALKTLRTLLERVG
                     LTQEDLF"
     gene            complement(6020..6763)
                     /locus_tag="AC06IO_002115"
     CDS             complement(6020..6763)
                     /locus_tag="AC06IO_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIO9875291.1"
                     /translation="MMRLFRKQLICIFFILSVAGCGTRSDLAPVTELKWQPYSKYQKF
                     HTVRSGETLYAIAFRYDTDYRRLAIINHLRPPYSLRVGQVLSLKGIVPKSNHFHSAPR
                     ANLHITHKPKQKIIYSPAYNVRSSSGWLWPVTGRVVTTFIPSQGKKGINIACNRGDKV
                     RASANGVVAYAGSGLPGYGNLIIIKHSNEYLTAYGNNARNLVTEGQRVSAGQVIAEAG
                     LIDRSYWGVHFEIRRAGVPVNPLNYLQKG"
     gene            complement(6782..7540)
                     /gene="surE"
                     /locus_tag="AC06IO_002116"
     CDS             complement(6782..7540)
                     /gene="surE"
                     /locus_tag="AC06IO_002116"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIO9875292.1"
                     /translation="MKILVSNDDGVLAPGIKILANELSTLGEVKVVAPDRNRSGASNS
                     LTLTQPLRVKQLDNGYYSVDGTPTDCVHLALTGFLEPMADIVVSGINEGANLGDDVLY
                     SGTVAAAMEGRYLGLPAIAISMVGDNIQHYETAAIIAKQLVIKLSANKLPSQTILNVN
                     VPDLPLNQIRGLQVTRLGTRHSAEPIIKEYDPRGRPIYWVGPPGIEADAGAGTDFFAI
                     KTGHVSITPLHLDMTHYKLFDHLSNLLNEICIEN"
     gene            complement(7543..7926)
                     /locus_tag="AC06IO_002117"
     CDS             complement(7543..7926)
                     /locus_tag="AC06IO_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875293.1"
                     /translation="MNKYLVIILITLGLVSCQFKKDESYYRSHPSELQNALKLCPGTQ
                     PDGLSCQQLEAIGRRMNSLAYQLQYNPQEFGNKILALQQVIANQQREMQSKKDNAELQ
                     DSLKRNQEDLAYYLAVVKWLESPES"
     gene            8006..9721
                     /locus_tag="AC06IO_002118"
     CDS             8006..9721
                     /locus_tag="AC06IO_002118"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIO9875294.1"
                     /translation="MLDFSLNRDPQTGELYIETSLCGKPLLTTPQLNKGTAFTQEERK
                     DFGLLGKLPHRVETLDEQVKRAYLQYSSYTTRLQQHIYLNNLHDKNQIVFYKLLSRHL
                     GEMLPIIYTPIVGAAAKRFSHEYRQPRGLYIAHSDKNQLEEIINNRSNPDIDLVVVTD
                     GEGVLGIGDQGIGGMDIPVAKLMVYSLCGGIDPTRTLPIFLDVGTNNQELLNDPMYLG
                     CRHPRIKSSEYDDFIKTFVNEIHKQFPNAFLHWEDFGRGNARRILDQFQDELCTFNDD
                     IQGTGAVTLAAILAACDVTGLPLHEHRIVVFGAGSAGTGISDQIVDAMVKNGLSLTDA
                     YDRFWLIDKQGLLLATDQELTDAQKPYARNPIDIQSWEINNKQHPSFTDTIRRVKPTI
                     LIGCSAQTGAFSQDIVETMSTACERPIIFPLSNPDEKCEAQPSDILAWSKGRALIATG
                     TAFAPIEYQNRMVQIAQCNNALVFPGIGLGVLAVSASRLTKDMILAAAQTLSKFAPSK
                     KDSFLPLLPSLDNAQIVAKEIAIAVAQCAIDSGYAQKNQDKELPRLIDELFWEPRYLP
                     FRKRG"
     gene            complement(9778..10149)
                     /locus_tag="AC06IO_002119"
     CDS             complement(9778..10149)
                     /locus_tag="AC06IO_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HIO9875295.1"
                     /translation="MKKLFSGLLMGFSALAYANNEVVINVSSKNPSFVVSLAANPTTG
                     YQWSVVEFDKNLLTLSNSIYEKPKTNLIGAGGKMLFTFNLNKGKTYPAQTKMVFKYAR
                     SWEPESGTIKNVTINFANPSD"
     gene            complement(10186..10503)
                     /locus_tag="AC06IO_002120"
     CDS             complement(10186..10503)
                     /locus_tag="AC06IO_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HIO9875296.1"
                     /translation="MIIRNYLLAIIFLMIVYPNAYAMRCGDKLVYEGDSKYTVLSKCG
                     EPLDKLVYEENVPLYNAAGYQIGYGTNTVETWIYQKSPVDFQYELIFDYGKLKQINVN
                     RNP"
     gene            complement(10586..12397)
                     /locus_tag="AC06IO_002121"
     CDS             complement(10586..12397)
                     /locus_tag="AC06IO_002121"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9875297.1"
                     /translation="MNSYNKKSEELPKTLGRFFWHFIKKQPLAFTVFFVAPIAMVLEN
                     NAIPYSLKMIIDALGVHSREENIFSAVAPALWLGGGAWFALIVILRLQNWWQGYVIPR
                     FQADVRMSVMEYLSRQSYHYFSNQMAGGLANKVNDLPRALDSIFNIITWYAIAAFASI
                     IVALILMCTINYWFAVILLSWIIFQLYISYRLSKKVDQYAKENAEDKSQLSGKIVDSL
                     SNASVVKLFARSRDELAYISASQNMEVQSNKKLTIYMNIFRLYLDIPVTVMLVAMVYF
                     LLTFWNKKLITTGDLVFIFNVSFSIMTQIWYLCHAIAEFFREIGIARQAIALLSAPIE
                     VDDAPDAKPLIVKEGKIEFDSVTFHYNQGKKVFENKSVIIKPKQRVGLVGFSGSGKTT
                     FIHLILRLFNVESGKILIDGQDISQVSQDSLRSAISLIPQDTTLFHRTLMENIRYGNP
                     DASDDEVVMASKQACCDDFISLLPDGYNTLVGERGIKLSGGQRQRVAIARAILKNTKI
                     LILDEATSQLDSLTEEAIQNSLWQLMESKTTIVIAHRLSTLLHMDRILVFEAGKIIED
                     GSHEELLAKDGLYKSMWDAQVGGFLPESDRDRDNWDE"
BASE COUNT         3799 a         2641 c         2180 g         3829 t
ORIGIN      
        1 aaaattattt ggataaattt aattttcagg tatggaccaa tggaggtaaa ttattattac
       61 attcacctac tgcccctaaa ataccattaa cagcagagac agacggcttt agcgataaaa
      121 gaatttctaa ccaggattgg cgagtattca caacttataa tgacaaagca ggaattcgta
      181 ctgtactggc agagcgttat gataccagaa atgagctggg tcacagaatt gcccaagatg
      241 acctctatat tatgttgctt acttttccat tatcaggact attaatatgg atcattattg
      301 gaagaggact agacagtctg gatagagtag cagaagaggt tgctaaccgt gcgccaagtc
      361 acctggaacc tgttgattta caagaagttc ccgaagaaat taaacctgta attgatgaat
      421 taaataaatt attttttcga ctcaaagaag ggtttgagcg tgaaaaacga tttgctgccg
      481 atgcagcaca tgaattgaga acacctcttg cagcacttaa ggctcaagct caagtggctt
      541 tgaactccaa tgatatagaa gaaaaaaatc aggctttaca aaaattaatt gccagcgtta
      601 atcgaagcac acatatagtg cagcagttgc taaccatgag taaacttgta ccagaagcaa
      661 cagaactaca tgagcaagat gaagtcaatc taagtaaatt aactcgtgaa atattagcca
      721 tgttggctcc gtctgcggtc gaaaaacaaa ttgagctgga atttgaaacc gatgaaaaaa
      781 tgcctaaaat ttatggaaat ccaacggctt tgggaatatt aatacgcaat ctggttgaca
      841 attccattcg atattccatg gaaaacggaa gagttattgt tcgattatta aaacataata
      901 acgaaatcat actcgaagta tgtgataatg gcccagggat accacctgaa ttacaaaaac
      961 gtgtttttga aagatttttt agagtattag ggaacaaaag cccgggtagc ggtctgggtt
     1021 tggctatcgt gcaacaaata tgcgaacttc atggtggaag ggtcgtgctt gattcaccta
     1081 aaattggcac aggcctaatt gtgagagttt atttgcccat caaaacaaaa ccaaaataaa
     1141 ctcaataatc aaatcataat cttgctgtaa ttaacctttt tagcttggag aatctgcttc
     1201 atgtttctgt gtatcggtcg cagtcttcgc ttcttcttcc ttcttttgag cattatagtt
     1261 cgcttccttt acactttttt tatgctcttt catctgttgg gaagtaacct tttctgtggt
     1321 atttgaagaa gctgcatcgg ctgtaatagc cgttgattgc tctgtgtttc tctcaattga
     1381 catacctggg gtcactttac ccttatcagt ttggtgttta tgagtattga cttgagaatc
     1441 agcagcattg acagcaaagg ttagcatcac acttaccata aaaccgatca ttcttttcat
     1501 aaatgctcct gtttattatc gagaatttta tcagttcgca gttacaaact catcgcgaag
     1561 aaagaatttg caaagcctca cgaatcaatt gctcacaact cttattgcca ttatcaattt
     1621 tattcacaac tttccacgcc tcttgtggtt tatagcccaa ggcctccaac gcgctgattg
     1681 cctcttgttg actatgagtg ctacctgcct gcttttggaa ggtatcggaa accgaaccat
     1741 caaattgctt tatactgtct ctcatttcaa ctactaagcg ctctgcagtt ttcttgccta
     1801 tgccaggtaa tttggttaat aaagcagcat tctcctggtg aatacactga ataaattctt
     1861 taggagaaat actggataaa acagtcatag ccagtttggg gcctacccca ttcactttaa
     1921 ttaatgatct gaataaagaa cgctcctctt tatccaggaa gccatacaat aacaaagcat
     1981 cttctcgaac tatggtatga atatgtaatc cgataggttg gttaccattt tctatctgaa
     2041 aaaaagtatt caaagatgtt tctacatcat atcctacgcc attgacatca agaaccaatt
     2101 ttcctggctg atgcttatct ataatttgac catggagcca gcctatcata gtctcatcct
     2161 cctgcgtttc gttcctcttg aaccgattaa ttttgacaaa ccattcctca tatggctatg
     2221 gcatattgct attgccaaag catcggcagc gtctttttga ggagctcgat tgagctgaag
     2281 aagctctaca accatatgac ttacttgatc tttttcagcg gccccataac caaccacact
     2341 ttgttttatt tctcttgctg aatactcaca gattttcaca cgatgcgagg ctgctgctac
     2401 catggcaacc ccccgcgcat gtcctaattt caaagctgaa ttaggattgt tatgcataaa
     2461 tatctgctca atagcaacct cagttgggga atagtgatcc attagctcac atattccatc
     2521 atatatttgc aataatttat gacttaattc cacatcgttt gatgtgcgaa tacaaccgct
     2581 atcgatgtac gcgattttac ggccagattc tttaatcaat ccatatcctg taacccgtga
     2641 gcctggatct atccctaata taatcgtcat taattcattg attctaatat tttttcagaa
     2701 aattcagcat tgctgtaaac ttcttggaca tcatccaaat cttccagcat atcaatcagt
     2761 ttaattaaac tttcagccgt ttcatcacta attggaacca aggtttgtgc acgcatagtt
     2821 aaatgagact gctcaacctc caaaccggca tcctgtaaag catttaagac ggtgtgataa
     2881 gcttctacag gggtaatgat ctctatctga ctgtcctcta cagcaacatc agaagcgcca
     2941 gcatcgattg caatctccat caccttatct tccggttgat tggaagccat gagaatttca
     3001 ccttgattag taaaaagata ggatactgaa ccatcagtgc ctaaattacc tccatgtttt
     3061 gaaaaagcat gtcgaacttc cgataccgtt cgatttttat tgtcagacaa acaatctacc
     3121 aatatagcaa caccacctgg cccgtaaccc tcataacgca tagctatcat tgcttcgcca
     3181 tcagctcccc ctacccctcg cttgacagca ttatcgatgg tatcgcgctt catattggca
     3241 ttcagcgctt ttttaacagc atctctcaaa cgtggattac ttgactcatc tcctcctccc
     3301 atacgtgcag ctacagtaat ttcacgaatt aatttagtaa atattttccc tcttttggca
     3361 tcctgtacgc ctttacggaa tttgatatta gcccatttac tatgaccagc cattacatca
     3421 cctctaaatg aatttttatt tggagattat acaatgctct aacaccaaaa agaatgacta
     3481 aagccatcga tgctctcgac aatttgaact tttttggact atgctagcac cataaaaaaa
     3541 tcttgaagga gattgaactg tgtatttgca aggatttggt aattatcacc acagcgaggc
     3601 tgttaaagga gcattacccc caaatcaaaa ctcacctcaa cactgtagct taggacttta
     3661 cgcagagcaa ttgagtggaa cctcgttcac ccgtccccga cataataatc ttcgaagttg
     3721 gctatataga atacttccta ctgttaccca gggcacgtat tacccctatg agtttaatat
     3781 tatgcaacct tttgttgatg agttgtcacc caatgccatg cgttggtcac ctctttataa
     3841 cagctctcaa attaaatgtg attttgttga aggactattt catattgctg gtagcccgtt
     3901 agttaatgcc tatacttatt attgcaacca ctccatgagc gataaatatt tcgccaataa
     3961 tgatggtgag ttattatttg ttccctatac aggcgagatt catctgcata ctgaatttgg
     4021 caaattaatg ctctcttctg gatcgatcgc agtgatacct cgtggcgtta aatttaaagt
     4081 ggaagtaatc agcaaagagg caaaaggtta tctttgtgaa aatagcggaa atcccttaac
     4141 cttacctcag ttaggcccca ttggggccaa tggtttggca aacccaagac attttcaata
     4201 tccagtagcc gcatttgaaa actctggagg cgagcatact ataatctgta aaaaccagaa
     4261 aaaattatgg tttactgtat gcaaccactc tcctttaaat gtcgtcgcct ggcatggcaa
     4321 ttatgcacca tattgttatg atctcagttt gttcaataca attaacacag tcagttttga
     4381 tcatcctgat ccttccatat tcactgtatt aacttcagaa agcgaaatac ccggtgtttc
     4441 taacttggac tttgttattt tcccacctcg ctggatggtt gctgaacata cttttagacc
     4501 gccttatttt catagaaact acatgaatga actgatggga cttgtctatg gtgaatatga
     4561 cgccaaaaag gaaggattca taccgggcgg tatcagcatc cataattgca tgactccaca
     4621 cggacctgat tatgaatctt atgaaattgc agcgtcgcag gatctaaaac caaattatat
     4681 caactccctg gcctttatgt ttgaaaccaa agactactgg caagtaactg agcaagctta
     4741 tctacatccc agcagacaaa tggattatct taattgttgg caaggcttta aattagagtt
     4801 tagtcaataa attgactgct ttttaagatg taaaattgac ggtaaataat taatttggtt
     4861 tcagttgggt caaatttgac ccaacttgta tgtaattaac tagttaaatc aaaacaaatc
     4921 ctcttgtgtt agtccaaccc gctctaacaa agttctaagt gtcttcaaag cttcaacttg
     4981 aatttggcga acgcgttctc gggtcaaatc aatttcctta ccaacgtctt ccagtgtcgc
     5041 tttttcaaaa cctcttaacc caaaacgtct tgcgataacc tgctgttgat tctcagttaa
     5101 tttatccaga agagattcta tatgctgcct taaattctcg ttggttagca gctctgcagg
     5161 atttaaactg ttttcatcag caattgtatc taataaagat ttggtttcat caaatccaat
     5221 tggagtatca accgaagcga ctttatcatt caaacccaat aatttttgga catcctccaa
     5281 aggcttatcc accatttctg ctatttcttc cggtgatggt tcatgatcca gtttttgcgt
     5341 caattgtctt gctgctctga gataaacatt aagttctttc acgacatgaa taggcaggcg
     5401 gatagttctc gtttgattca taattgcgcg ctcaatagtt tgcctaatcc accaggtcgc
     5461 ataagttgaa aaacggaatc ctcgcttggg gtcaaatttc tcaacagact tcattaaacc
     5521 cagattaccc tcttcgatta gatccagaag aggtaatcct ctattaagat aacgtctggc
     5581 tattttaacc accaagcgca aattcgactc aatcattttc ttgcgtgctt ccatatcacc
     5641 ttttaaagca agagtcgcat aatggatttc ttcttcagcc gttagcaaag gagaaaaacc
     5701 aatttcaccc aagtaaagtt gagtcgcatc taaagccttg gcaatatttt tcccgcgctg
     5761 gaatgaagga aaagcttcat catcagtaaa ctctggaagt tcatcaagct cttcttccat
     5821 tttttcgata tcaggttcaa tatcctcttc tgacaacaga gagtcatcat ctggctcaga
     5881 ccattcctct tctttaaatt ccttgtcctt aattggctct tcttcatctt gcattgtatc
     5941 acctatatgt ttagctatgc cttgaaatag ttttttactt cttaacaagc ctattgttgc
     6001 tataactatg agttatatgt caaccttttt gtagataatt caatggatta actggcaccc
     6061 ctgctcttct aatttcaaaa tgaacacccc agtatgatct atctattaaa ccagcttctg
     6121 ctattacctg ccccgcacta acacgctgcc cttctgtcac cagatttctg gcattgttac
     6181 cgtatgcagt taaatattca ttactgtgtt taataatgat taaattacca tacccaggca
     6241 aaccgctgcc agcataagcc acaactccat tcgctgaagc tctaactttg tctcctctgt
     6301 tacatgcaat atttatgcct tttttcccct gagaaggaat gaatgttgta acaacacgac
     6361 cagtaactgg ccacagccaa ccagaggaag aacgtacatt ataagctgga gaataaataa
     6421 ttttttgttt tggcttatgg gtaatatgta agttagctct gggagctgaa tgaaagtgat
     6481 ttgattttgg cacgatgcct tttaaactta aaacttgtcc tactcgcaag gaataaggtg
     6541 gccttagatg attgatgatc gcaagccgcc tataatcagt atcataacga aatgctatag
     6601 cataaagggt ctcacctgat cgcaccgtat ggaatttctg atattttgaa taaggctgcc
     6661 attttagctc ggtaacagga gccaaatcac ttcttgtccc acaaccagca acacttaaga
     6721 taaaaaaaat acaaattaat tgtttgcgaa ataggcgcat cattatctca tttattattt
     6781 attaattttc tatgcaaatt tcgttaagta agttagataa atgatcaaat agcttataat
     6841 gagtcatgtc taaatgaagc ggtgttatag aaacatgacc tgttttaatt gcaaaaaaat
     6901 cagtaccagc gccagcatct gcttctatac ccggtggacc aacccaataa attggccttc
     6961 ctctgggatc atattcttta ataataggct cagcgctatg gcgagttccc aatcgcgtaa
     7021 cctgcaatcc tcttatttga ttcaaaggta aatcaggaac attaacattt aatatagttt
     7081 gagaaggtaa tttgttagca cttaatttta taaccaattg tttggcaata attgcagctg
     7141 tttcataatg ttgtatgttg tcccctacca ttgagattgc aatagcaggc aaccctaaat
     7201 aacgaccttc catagccgcc gccactgtac ctgagtaaag tacatcatcc cctaaattgg
     7261 caccctcatt aatccctgaa acaactatgt ctgccatagg ctctaaaaat ccggttaacg
     7321 ccagatgaac acaatcagta ggtgttccat ccacactata ataaccattg tccaattgtt
     7381 taactcttaa aggttgtgtt aaagtcaaag aattactggc gccacttcta tttctatcag
     7441 gcgcaaccac tttaacttct cctaaagtgg acagctcatt agctaaaatt ttgatcccgg
     7501 gcgccaaaac cccgtcgtca ttgcttacta atattttcat gtttaactct caggggattc
     7561 taaccatttt acaactgcta aatagtaagc aagatcttct tgatttcttt tcaatgaatc
     7621 ttgtaattca gcgttatcct ttttactctg catttcccgt tgctgattgg caataacctg
     7681 ctgcaaagcc aaaattttgt taccaaactc ttgtggattg tattgtagtt gataagcaag
     7741 actgttcatc ctcctgccta ttgcctccag ttgctggcaa cttaatccat cgggttgcgt
     7801 gccagggcac aattttaaag cattttgcag ttcggatggg tgggaacgat aataggattc
     7861 gtctttttta aactgacatg aaaccaatcc caaagttatt aatattatca ctaagtactt
     7921 attcataccc gccctcttga tgtaaactac aaaaagtcga tattatcacc agtcagcaag
     7981 taacgctatc ttatgagaat accatatgct tgattttagt ttgaatcgtg atccccaaac
     8041 gggcgagtta tacatagaaa cctcattatg tgggaagcct ttattgacta cgccgcaatt
     8101 aaacaaagga acagcattta ctcaggaaga aagaaaagat ttcggtcttt taggcaagtt
     8161 accacaccgc gtagaaactc ttgatgaaca agtaaaacgt gcctatcttc aatattcaag
     8221 ttacacgacg cgtttgcagc aacatattta tttaaataat ttacatgata aaaaccaaat
     8281 agtattttac aaactgctaa gtcgtcattt gggggaaatg cttcccatca tctatacacc
     8341 aatcgttggt gctgcagcca agaggtttag tcatgaatac agacagccac gaggattgta
     8401 tattgctcat tcggataaaa accagcttga ggaaataatt aataaccgtt cgaatccaga
     8461 catagacctt gttgtagtga ccgatggtga gggagtccta ggcattggcg atcaagggat
     8521 aggaggaatg gatatacctg ttgccaaact aatggtttac agcttatgtg gtggcattga
     8581 tccaactcgc acattaccca tatttcttga tgtaggaact aacaaccaag aattattaaa
     8641 tgaccctatg tatctgggtt gccgtcaccc cagaataaaa agctcagaat acgacgattt
     8701 tatcaaaact tttgtcaatg aaattcataa gcaatttcca aacgcttttt tacattggga
     8761 agactttggt cggggcaatg ctcgacgaat cctggatcaa tttcaagatg aattgtgtac
     8821 ttttaatgat gatattcagg gaactggtgc agtaactctg gcagcaattt tggcagcatg
     8881 tgatgttact ggattaccct tacatgaaca cagaatcgtt gtgtttggag ctggctcagc
     8941 aggtactggt attagcgacc aaattgttga tgcgatggtg aaaaacggat tgagcctaac
     9001 tgatgcttat gacagattct ggttaataga caaacagggt ttattacttg cgactgacca
     9061 agagcttact gatgctcaaa aaccctatgc acggaatcct atagatatac aatcctggga
     9121 aattaataat aaacaacatc cttcgtttac tgacaccatc aggcgtgtca aacctaccat
     9181 tttaattggc tgttcagcgc aaacgggtgc attctcacaa gatattgtgg aaactatgtc
     9241 gacagcctgc gagcgaccaa tcattttccc tttatcaaat cctgatgaaa aatgtgaagc
     9301 acagccctca gatatattgg catggagtaa aggacgggca ttaattgcta ctggaactgc
     9361 ttttgcccct attgaatatc agaaccgaat ggtgcagata gctcaatgca acaatgcctt
     9421 ggtattccct ggaattggat tgggcgtttt ggcggtaagc gcctctcgtt taactaaaga
     9481 tatgatattg gctgccgccc agactttaag taaatttgcg ccaagtaaaa aggacagctt
     9541 tttgcccctg ttaccctcat tagacaatgc gcaaattgta gccaaagaaa tagctattgc
     9601 agtagctcag tgtgcaatag actcaggtta tgctcaaaaa aatcaagaca aggagctgcc
     9661 tcgattaatt gatgaactct tttgggagcc tagatatctg ccttttagaa aaaggggata
     9721 gagcgaataa aataaagctc tatgactata ttattgaaaa ataaattttc ttaattctta
     9781 gtctgagggg ttggcgaaat taatcgttac attttttata gtcccgctct ctggctccca
     9841 ggaacgagcg tatttaaaga ccattttggt ttgagcggga taggttttac ctttattcaa
     9901 gttaaaagta aatagcattt taccgcccgc acctatcaaa ttagtcttgg gcttttcata
     9961 aatactatta cttaaggtaa gcaaattttt gtcaaattct actacagacc attgataacc
    10021 tgtagttgga ttagctgcaa gactgacaac gaaactggga ttttttgagc tgacattgat
    10081 gacaacctcg ttatttgcat aggccagagc agagaagccc ataagtaatc cgctgaataa
    10141 ttttttcata ttttttccct caaaaagctt cttacacgct caacattatg ggttacgatt
    10201 tacgttaatc tgttttaatt tcccataatc aaaaattaat tcgtattgaa aatcaacagg
    10261 agatttttga taaatccagg tttcgactgt attagtacca taacctatct gataaccagc
    10321 cgcgttgtat aatggcacat tctcttcata gactaattta tcaagtggct caccacattt
    10381 agacaaaaca gtgtatttac tatccccttc atagaccaat ttatctccac accgcattgc
    10441 atacgcatta ggatatacaa tcattaaaaa gataatagcc aacaaataat tccggataat
    10501 cattccctac cccttgagaa ttcaatgctc cttgcaacaa aattttcaaa aagtctattt
    10561 ttatagcata gcacttggta tctgattatt catcccaatt gtctctgtct cgatcggatt
    10621 caggcaagaa tccacctact tgagcatccc acatggattt atataaacca tccttagcta
    10681 ataactcttc atggcttcca tcttcaatta tttttcctgc ttcaaaaact aaaattctat
    10741 ccatatgcaa tagtgttgat aatcggtggg caataacaat tgtagtcttt gattccatga
    10801 gttgccacaa tgaattttgt atggcttctt cggttaggga atccagctgt gaagtagctt
    10861 catctaatat caaaatcttc gtatttttta aaatagcgcg agcaattgca acgcgttgtc
    10921 tttgcccccc tgatagttta atcccccttt cacccaccaa agtattatag ccatcgggta
    10981 aaagtgaaat aaaatcgtca cagcatgcct gttttgatgc catcacaacc tcatcatcac
    11041 tcgcatcggg gttaccataa cggatgtttt ccataagagt cctatgaaac aatgtagtat
    11101 cttgagggat aaggctgata gctgaacgca aggaatcttg agacacttgg ctgatgtcct
    11161 gaccatcaat caaaattttt ccggattcca cattgaataa acgtaaaatt aaatggataa
    11221 acgtcgtttt accacttccc gaaaaaccta ccaagccaac acgttgttta ggtttaatga
    11281 tcactgattt attttcaaat actttcttac cctggttata atgaaaagtt acgctatcaa
    11341 actctatttt tccttcttta acaatcaatg gcttggcatc aggagcatcg tctacctcta
    11401 tcggtgcact aagcaaggca atagcttgtc ttgctatgcc tatctctcga aagaactcgg
    11461 caatagcatg gcacaaatac caaatttgag tcatgattga aaacgataca ttgaatataa
    11521 agaccaaatc tcctgttgta atgagttttt tattccaaaa agtcaacaag aaatacacca
    11581 tagccactaa catcactgtg actggaatat cgaggtacaa acgaaatatg ttcatataaa
    11641 tcgtcagctt tttattgctt tgtacctcca tattctgact tgcggaaata taagccagct
    11701 catcgcgaga acgagcgaaa agcttcacca cactggcatt actcaaactg tcaactattt
    11761 taccgcttaa ctgactttta tcctccgcgt tttcttttgc atattgatca acttttttcg
    11821 acaatctata actgatataa agctgaaaaa taatccagct taacaaaata actgcaaacc
    11881 aatagttaat ggtacacatc aaaatcaatg ccacaatgat agaagcgaat gctgcgatcg
    11941 cataccaggt tatgatattg aatatggaat cgagagcacg gggtaaatca ttgactttgt
    12001 tagccaaacc acccgccatt tgattggaaa aataatgata agactggcga cttaaatact
    12061 ccatgacaga cattctgaca tcagcctgaa atcttggtat cacataacct tgccaccaat
    12121 tttgcagacg taaaataaca attaaggcga accaggcacc tccacctaac cataaagccg
    12181 gagcaactgc tgaaaaaata ttttcttctc tggagtgcac cccaagagca tcaatgatca
    12241 ttttcaaaga atagggaata gcattatttt caagcaccat agcaatcggg gctacaaaaa
    12301 acacagtgaa agccaaaggc tgtttcttta taaaatgcca gaaaaaccga cccagtgttt
    12361 ttggcaactc ttcagatttt ttgttatagc tattcataaa ccataccttc aatgaatttt
    12421 gacaaggttc gattattcta acctaatat
//
LOCUS       DBNCWK010000094        12264 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.094, whole genome shotgun sequence.
ACCESSION   DBNCWK010000094 DBNCWK010000000
VERSION     DBNCWK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.094"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     regulatory      53..146
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            221..1165
                     /locus_tag="AC06IO_002122"
     CDS             221..1165
                     /locus_tag="AC06IO_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9875298.1"
                     /translation="MSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEP
                     ADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGIN
                     SFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPTSYKTVRIGMNVTDAIYRDVIDTGIG
                     FINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQ
                     RGAAYTTEKPEEAYELLCQAKPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYT
                     KHLKIIDEHFDISQCYTNRFLPDTPYSDLKPIACCLEN"
     gene            1168..2238
                     /locus_tag="AC06IO_002123"
     CDS             1168..2238
                     /locus_tag="AC06IO_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9875299.1"
                     /translation="MRAGIVGAGIMGRLLAFALHNAGWQVSLFDRSNENMNCSTSAAG
                     LLTPFSELDKSTLLISTLGQESIKDCWPRIIEDLPEAVYFQQSGSLVLCHPGDQAEWI
                     HFSRRILSQLNLSNSVFQKLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKN
                     YLDSVCITHITNTPVLSLKPGEINTKEQRYHFDVVFDCRGLGAQNVFSDLRGIRGELI
                     WLHAPGVQLHRPIRLLHPRYSLYLVPRPGNLYLIGASEIEAEDFSPVSVRTTLELLTA
                     VYYLHGNFDEARIIKTVTHCRPTLANHLPRIKFADGLVAINGLYRHGYLIAPALAEEI
                     LRGVTSNFRHLNYPELWETYCD"
     gene            2231..2434
                     /gene="thiS"
                     /locus_tag="AC06IO_002124"
     CDS             2231..2434
                     /gene="thiS"
                     /locus_tag="AC06IO_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIO9875300.1"
                     /translation="MINVYLNDKIYNIESSLSLSVFLKQLNYTQQHFAVAINNHHVPH
                     KAYETTILQANDCIDILFPMQGG"
     gene            2437..3225
                     /locus_tag="AC06IO_002125"
     CDS             2437..3225
                     /locus_tag="AC06IO_002125"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIO9875301.1"
                     /translation="MWNLAGKKLSSRLLLGTACYPSLEHMQQAIHNSGTEVITISIKR
                     QTSAGLDGESFWQAVKKLDCHLLPNTAGCRNAEAAINTAEIARELFNTHWIKLEVIGD
                     DYNLQPEPFELIKAARILIDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGK
                     GLINPYALETLRYRFPDITLIIDAGIGKPSHAVQVMELGFDGVLLNTAVALANHPALM
                     ATAFRHAVIAGHQAFTGGMMSERNVAHPSTPLIDTPFWHQVNNL"
     gene            3222..4688
                     /gene="thiD"
                     /locus_tag="AC06IO_002126"
     CDS             3222..4688
                     /gene="thiD"
                     /locus_tag="AC06IO_002126"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIO9875302.1"
                     /translation="MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQ
                     NAHSISAVEAISRDQVAAQCRALELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVV
                     LDPIITSSSGTNLFFPDLQQHKKNLIQLFPYITIITPNRIEAEIILNRSISSYQDIIN
                     AALDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKESFWIANRRFPETNYRGTGCALSS
                     ALTACLALGYSMKDAIVIAKMYVSRGIRQSIEIDKEASQLYHDGWPEDEADLPYLSPT
                     PFIKPVPSFKKYSMGFYPIVDSSHWLEMLLPLGIKCIQLRIKEASQERLEEEIKRSVH
                     LANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRAGLFLGISTHCYYEV
                     ARAHALNPSYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSD
                     VLKTKIDGVSVISAITKASAPLVAAKQFLTQMNESYNE"
     gene            4681..5844
                     /locus_tag="AC06IO_002127"
     CDS             4681..5844
                     /locus_tag="AC06IO_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIO9875303.1"
                     /translation="MNKSNLAMNELTRYSQQIKMEEIGLDGQEKLKNSRVLCIGLGGL
                     GSPLLLYLAAAGVGVIGIVDDDIIELSNLHRQILYNHAHINKKKAVTAKKQLLAINPL
                     IQVESYSSRLTEENAADLITQYDIIADGTDNFYTRYLIHSICFELGKPYVYASASGFQ
                     GYCSIFHGKQAPCLHCIFPVSVSDINMLNCEGAGVIGTLPGMLGIIQATEIIKWILQI
                     GNPLKKRLLAINLLTMTFKNIIISRNPDCEFCIQNQSIRQLINPVNCSTQIELCHHRI
                     TFDEFYQLLTNNKNIHLIDVRSTKEHQIQNIGGIVLPLDELPQRLHELNPNQLIILYC
                     QSGKRSKMALDILLKAGFASVKYIENGLKGVTANYNKGMPQKFTPPKKEAEIK"
     gene            complement(5837..6670)
                     /locus_tag="AC06IO_002128"
     CDS             complement(5837..6670)
                     /locus_tag="AC06IO_002128"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HIO9875304.1"
                     /translation="MIRSILVLFFLFIANAGYAWNAIGHQLVAQIAYDNLTPQSRRMC
                     DLYSHSKSKTSSNVNFVKSASWLDSIRAHDVHWFDALHYIDIPFSMDETELPVLTDIN
                     ALWGINQAIAVLSSKKASIADKKLSLRILVHLVGDIHQPLHTVTKISKKLPKGDLGGN
                     LFQLAKNPIGNNLHQYWDNGGGILIGQDKFFQIKNKARQLEKKWSCQSVSKEKNPQQW
                     INASHQLALTKVYKVSTRQVPGKQYQLNTQNITEKQILLAGCRLAYLLNNIAEGKNKL
                     I"
     gene            complement(6673..7932)
                     /gene="tolB"
                     /locus_tag="AC06IO_002129"
     CDS             complement(6673..7932)
                     /gene="tolB"
                     /locus_tag="AC06IO_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIO9875305.1"
                     /translation="MFNRIISLFLLLFTGQVIALDLELTQGINSALPIAINSFGSDAA
                     AQEIGNVIENDLTISGQFKIISGPQGANSQSSVSTLRQLGADSVVTGRVNQVGNRIEV
                     SFTLADAVANGNILLTKTFQINANQVRALAHHISDEVYQKLTGERGIFSTRIAYISVQ
                     RAGGRSRYSLEVADADGHNPQSLLVSSEPIMSPSWSPNGKSISYVSFEKKKAEIFTVS
                     VETGQRRLITSFPGINGAPAWSPDGQHLAVVLSKSGTPKIYDVDLSSGSMKQLTFGNS
                     IDTEPRYSPDGRSLLFTSGRGGSPQVYRLSLADGQISRVTFEGNYNARASYTPDMKHI
                     VMLHREDRQFNIGVQNTGGGPISNLTFSGLDESPSVSPNSRLVLYATRYQDRGVLGIV
                     SIDGRIRMRLPAREGDVQEPAWSPYLS"
     gene            complement(7938..8936)
                     /gene="tolA"
                     /locus_tag="AC06IO_002130"
     CDS             complement(7938..8936)
                     /gene="tolA"
                     /locus_tag="AC06IO_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIO9875306.1"
                     /translation="MISDLTYRKAFLFAVTLHLFLLVLLFTDNSSKRPVLTMESKNSP
                     GMMQPTASQQEVVKAVSVDNKEIMKTVNRLKQEREQQKKAEINRQKELLRQAEAAKQQ
                     RIKEQQEIARLKEEANKIAIARKKQVEEEKKRLKELAEQKALEAKRIEELKKQNEKLA
                     KQRELEAKKLAELNKKKQEEKEKAEKLKAEQAKAEKLKAEKARAEQEKAEMERKRQAE
                     AAAAQQAAQNAERQARIAGEVDKYKALIVNAIGRNWILPENVDSSLSSQFRIRLAPDG
                     TVLEVSLTRSSGDPLLDRSAQTAIYKASPLPVPKDVETFNLFRDISLTVRPEQVRG"
     gene            complement(8933..9388)
                     /gene="tolR"
                     /locus_tag="AC06IO_002131"
     CDS             complement(8933..9388)
                     /gene="tolR"
                     /locus_tag="AC06IO_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIO9875307.1"
                     /translation="MNRSKTNRERPISEINVVPYIDVMLVLLVIFMITAPMLTQGVTV
                     DLPKAASQTLQAADREPIIVSVNQLGSYFLNISSTPAEPIEAQALLVRVAAELELAKQ
                     SGQKLNVLVKGDQGVAYGKVVQAMALLKQAGAEQVGLLTDSQTGDEGQA"
     gene            complement(9397..10074)
                     /gene="tolQ"
                     /locus_tag="AC06IO_002132"
     CDS             complement(9397..10074)
                     /gene="tolQ"
                     /locus_tag="AC06IO_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIO9875308.1"
                     /translation="MVANQANVLMYFMQAGLVVKSVMLILLIASIISWTLIFQRAWFF
                     NRRKQMTETFTRRFWDCGDLSKLYADVDSNSDERQGVAAIFHAGFKEFVRCRKQGKVS
                     IEPIQRVMQISHAKEAEKLEQHLPFFASVGSISPYVGLFGTVWGIMTSFQALGHAQQA
                     TIAMVAPGISEALVATALGLFTAIPAVIAYNRYTTRANTLLNKYDLFQEELISLIEQQ
                     TSGSPRG"
     gene            complement(10099..10494)
                     /gene="ybgC"
                     /locus_tag="AC06IO_002133"
     CDS             complement(10099..10494)
                     /gene="ybgC"
                     /locus_tag="AC06IO_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIO9875309.1"
                     /translation="MNLISNHQHNIRVYAEDVDYMGIVYHANYLCYLERARTELLRQN
                     NLILSEFEKSDILFAIHELTINYVFPARLDDQLTIKTEIKEFRACSFLFNQEISNQHK
                     QLICRASVQVVCVGSNLKPKRLPNIMRNE"
     gene            complement(10498..11508)
                     /gene="ruvB"
                     /locus_tag="AC06IO_002134"
     CDS             complement(10498..11508)
                     /gene="ruvB"
                     /locus_tag="AC06IO_002134"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIO9875310.1"
                     /translation="MLESDRLISSQSIVSEDAMDRAIRPLSLSEYVGQDSVSSQMQIF
                     INAARKRNDPLDHVLIFGPPGLGKTTLANIIAHEMGVNIRQTSGPVIERAGDIAAILT
                     NLQQNDVLFIDEIHRLSPVIEEILYPAMEDYKLDIMIGEGPAARSIKLELPPFTLIGA
                     TTRAGLLTSPLRDRFGIVQRLEYYSVDSLTQIVARSAHLLGVPTKPEGAREIALRSRG
                     TPRIANRLLRRVRDYSEVKGNGIITVDMAQQALEMLEVDQHGFDLMDRKLLLAVIEHF
                     NGGPVGIDSIAAAIGEEKGTIEDVLEPFLIQQGFLMRTPRGRIATSKAYQHFGFSAIE
                     QE"
     gene            complement(11655..12218)
                     /locus_tag="AC06IO_002135"
     CDS             complement(11655..12218)
                     /locus_tag="AC06IO_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIO9875311.1"
                     /translation="MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRRMIYFYTH
                     DWTNVNDLTQEVLIKVFRHLPYFNERSQFSTWLYKITQNTIKNYFRTNNLYADSEMQY
                     MDEQFSTPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMH
                     CPIGTVRSRIFRARKQLMDIVSENHTL"
BASE COUNT         3579 a         2464 c         2328 g         3893 t
ORIGIN      
        1 attatcgcta gtttgacaaa gcatataata aaatttagta tatttgagtt tgttagatgg
       61 cgcggggtgt cgggaaatcc ggctgagagt tacccgttga acttgatctg aatcatatca
      121 gcgtaaggac gccttcaaaa aaaccatggc ctttttaagg catggttccc ttttgccatc
      181 tccataatat ttaaaaataa taacataagg agttatggcg atgtcatcac taaaatccag
      241 ggttacccta ctgcttaatt ggtacaccaa cccttatcat acccccatac ttgtggcaca
      301 acaattggga ttttattcag aagaagatat taagctcgcc attctggagc ctgcagatcc
      361 cagtgatgtg accgaaattg ttgggctggg aacagtagat tttggtgtta aagcaatgat
      421 tcataccgtt gcagccaaag caaaaggtta tccagttact tccataggaa cgcttcttga
      481 tgagccacct accggactta ttgctttaaa atcaagtggt atcaattctt tccaggatat
      541 tgttgggaag cgagtaggct atatcggcga attcggcaaa aaaattattg atgatttggc
      601 cagcctcgca ggtattgatc cgacgagcta caaaacagtc cgtattggca tgaatgtcac
      661 agatgccatt tatcgggatg ttatcgacac tggtattggt tttattaatt ttcaaaaagt
      721 tgaactggag catctttgcg gagaaactgt atttttacga atcgatcaat tagctggttt
      781 aggctgttgt tgtttctgct caatacaatt tattgttcct gaaacaacat tacagcaacc
      841 tgaattagtc aaaggatttc tcagagcaac acagcgcggc gcagcctaca cgacagaaaa
      901 acctgaagaa gcttatgagt tattatgtca ggctaaacca cagttgcgga ctcctttata
      961 tcaaaaaatt tttacaagaa cattaccatt tttttcacgt acattaatta atgttgacag
     1021 agactgggat aaggttggac gctataccaa gcatctaaaa attattgatg aacattttga
     1081 tatctcgcaa tgctatacga atcgtttttt gccagataca ccttattctg atttgaaacc
     1141 aatcgcctgt tgtcttgaaa attaattatg cgagcaggca ttgtaggtgc agggatcatg
     1201 gggcgtctgc tggcttttgc attacataat gcgggctggc aggtatctct ttttgatcgc
     1261 tctaatgaaa acatgaattg cagtacttct gctgcaggtc tattaacccc gttttctgaa
     1321 ttggataaat caactctatt gatttccacc ttgggtcaag aatctattaa ggactgttgg
     1381 ccacgtatta ttgaggattt gcctgaagct gtatattttc aacaatcggg cagtctggtg
     1441 ctatgccatc caggcgatca ggcagaatgg attcacttta gtcgacgtat tctgagtcaa
     1501 ctcaatctga gtaattctgt ttttcaaaaa ttatcccatg aaaatctcat tgcgcttgag
     1561 cctgagctga caaagtttga aactgcttat tattttccaa atgaagcgca tattgattgc
     1621 caaacactaa tgttgtcttt aaaaaattat cttgactctg tttgcataac tcacattacc
     1681 aatactcctg tcttatcgct taaacctgga gagatcaata caaaagaaca aagatatcat
     1741 tttgatgtcg ttttcgattg tcgtggttta ggtgcgcaaa atgtattttc tgatttacga
     1801 ggtattagag gcgaactcat ttggcttcat gcgccaggag ttcaactaca tcggcctatt
     1861 cgtcttctgc atccaaggta cagtctatat cttgtaccca gacctggaaa tctttacctc
     1921 ataggcgcta gcgaaattga ggcagaagat tttagtccgg tttcagtacg tacgactttg
     1981 gagttattga ccgctgtgta ttatttacat gggaactttg atgaagcacg catcataaaa
     2041 acggtgaccc attgccgtcc aactttggca aatcatttgc cacgtattaa atttgccgac
     2101 gggctagtag caattaacgg tctttatcgt catggttatt taatagcccc agccttggca
     2161 gaagaaattt tacgaggtgt gacatccaat ttccggcatc tgaattatcc agaactttgg
     2221 gagacttatt gtgattaatg tctatttaaa cgataaaatt tataacattg aatcttcatt
     2281 atcattaagt gtatttttaa aacagcttaa ttatactcaa caacattttg ctgtcgctat
     2341 aaataatcat catgtaccac ataaagctta tgaaactaca attttgcaag ccaatgactg
     2401 cattgatatc ctctttccca tgcaaggagg ttaataatgt ggaatttggc aggaaaaaaa
     2461 ttaagtagcc gtttgctctt aggtactgcg tgttatcctt cgttagagca tatgcagcag
     2521 gcaattcata attcgggtac agaagttatt acgatatcaa taaaacgcca aacttctgca
     2581 ggattggatg gagagtcctt ttggcaagcg gtaaaaaaac tagattgtca tcttttgcct
     2641 aatacggctg gttgccgtaa tgcggaggca gcgatcaata cagctgaaat tgcgagggag
     2701 ttatttaata ctcactggat aaaactggaa gtgataggcg atgattataa tttacagccc
     2761 gaaccatttg agttaatcaa ggcagccaga attttgattg acagaggatt tgaagttttt
     2821 ccttattgca cagatgatct cgttttatgt caaaaactgg ttgatgctgg ttgtaaaatt
     2881 ctaatgccat ggggagctcc tataggctca ggtaagggtt taattaaccc ctatgccttg
     2941 gaaactttgc gttatcgatt tcctgacatc acattaatca ttgatgccgg gattggtaaa
     3001 ccatctcatg ctgtacaggt gatggaattg ggttttgatg gcgtgcttct aaatactgct
     3061 gtagctcttg ccaatcatcc tgctctcatg gcaaccgcat tccgccatgc cgttattgca
     3121 ggtcatcagg catttacagg aggaatgatg tctgagagaa atgtagctca cccaagtact
     3181 ccattaatcg atactccatt ttggcatcag gtaaacaatt tatgaaaaaa ccgattgttt
     3241 ggacaattgc tggtgttgac tcatccggat tagctggtgt ccatgctgac atggaaactt
     3301 ttagtcgctt gaatgtcagg gcttgttcag tcatcaccgc agtaactgca caaaatgctc
     3361 actcaatttc agcagtagaa gcgatatcca gagatcaggt tgctgctcag tgccgtgcct
     3421 tggaattaaa tttaaaaccg gatgccatta aaattggtat gttatgttct acaccaatct
     3481 gtgaggaaat agcttatttt ttaaagggtt atgaaggatt tgttgttctt gacccgataa
     3541 ttacctcctc ctcgggaact aacctgtttt ttccagattt acaacagcat aaaaaaaatt
     3601 taattcaatt gtttccgtac attactatta ttactcctaa tcggatagaa gcagaaatta
     3661 ttttaaatcg gagcatttct tcttatcagg atataatcaa tgctgcttta gatctattat
     3721 cactgggagc aaagcaggta ttactgaaag gaggacatgt aaaagataac tcattcagtc
     3781 aagattactg gactgatgga aaagagtcgt tttggattgc aaatcgtcgt tttcctgaaa
     3841 caaattaccg tgggacaggc tgtgctctat cttcagcact caccgcatgt cttgcgcttg
     3901 gctattcaat gaaagatgct atcgttattg ctaaaatgta tgtcagtcgc ggcatacgtc
     3961 aatcaataga aattgataaa gaggcctcgc aactttatca tgatggttgg ccagaagatg
     4021 aggccgatct cccctattta tcaccaacac cgttcattaa accagttcct tcatttaaaa
     4081 aatattctat gggtttttat cccattgtgg atagtagtca ctggttagaa atgcttttgc
     4141 cattgggaat taagtgcatt cagttacgaa taaaggaagc ctctcaagag cggttggaag
     4201 aagagataaa acggagtgtc catttggcaa atcagtataa tgctgcatta tttattaatg
     4261 atcactggga acttgctatt cattatggtg ctgcaggagt tcaccttggc caagaggatc
     4321 tggaaaaagc tgatgtcgat agaattaatc gagcaggttt attcttaggc ataagtacgc
     4381 attgctatta cgaagttgct cgggctcatg ctcttaaccc ttcctacgtc gcttgtggcc
     4441 ccatatatga aacaaccagc aaaataatgc catttcaagc acaaggaatt gcacgacttg
     4501 aacgttggcg aaaaacgctt cgttatccat tggttgccat tggaggtata actttaaaga
     4561 acctttctga tgtcttaaaa acaaaaattg atggtgtgtc agtgatctct gccattacaa
     4621 aagcatcagc acctttagtt gcagccaaac aatttttaac tcaaatgaat gaatcatata
     4681 atgaataaat caaatttagc aatgaacgaa ttaacccgct attctcaaca aataaagatg
     4741 gaagaaattg gattagacgg acaggagaaa cttaagaatt ctcgagtatt atgcattggg
     4801 cttggcggtt tgggttcgcc tctcttactt taccttgctg cagcaggtgt aggagtgata
     4861 gggattgtcg atgatgacat cattgaacta agtaatttac ataggcagat tctttataat
     4921 catgcccata ttaataaaaa aaaggctgtt actgcaaaaa agcaacttct ggctattaat
     4981 cccctgattc aagttgaatc ttactccagt agattaacag aggagaatgc agctgactta
     5041 attacgcagt atgacatcat cgcagatgga accgataatt tttatacacg atatttgatt
     5101 cacagcattt gctttgagct gggaaaacct tatgtgtatg ctagcgcctc aggttttcaa
     5161 gggtattgct ctatattcca cggtaaacaa gctccctgcc tccattgtat cttcccggtt
     5221 tctgtaagcg atatcaatat gctcaattgc gagggagccg gggtaattgg tactttaccc
     5281 gggatgctgg gtattattca ggcaacagaa ataatcaaat ggatactaca aattggcaat
     5341 ccattaaaaa agcgtttgct tgctattaac ttgttaacaa tgacctttaa gaacataatt
     5401 atttcaagaa atccggattg tgaattctgc attcagaatc agtcaattag acaattaatt
     5461 aatcctgtta attgttcaac tcaaattgag ctatgtcacc atagaataac ttttgatgaa
     5521 ttttatcaat tactcacgaa taataaaaac attcatctaa ttgatgttcg tagtacaaag
     5581 gaacatcaaa tacaaaatat aggaggtatt gtgcttcctt tagatgagct gcctcaacgc
     5641 ttacatgaac taaaccctaa tcagcttatt attctgtact gccagtcggg aaaacgaagc
     5701 aagatggcgt tagatatact tctcaaggct ggctttgctt ctgttaaata tatagaaaat
     5761 ggtttaaagg gagtaactgc caattacaat aaaggcatgc cccaaaaatt cacccctcct
     5821 aaaaaggaag ctgagattaa ataagtttat ttttaccttc ggcaatatta tttagcagat
     5881 aagcgagtcg acacccagcc agaagtatct gtttttctgt aatattttga gtatttaact
     5941 gatattgctt gccgggcacc tgacgagttg aaaccttata aactttagtc aatgccaatt
     6001 gatgtgatgc attaatccat tgttgcggat ttttttcctt actgactgat tggcaagacc
     6061 atttcttctc caattgcctc gctttgtttt taatctgaaa aaatttgtct tgaccaatca
     6121 gaataccgcc accattatcc caatattgat gcaaattatt cccaatagga tttttagcta
     6181 actgaaataa attaccacct aaatcacctt taggtaattt tttacttatc tttgtaacgg
     6241 tatgcaaagg ctgatgaata tctccaacca aatgcaccaa tatgcgtaag ctcaattttt
     6301 tatcagcaat actggctttt ttggaagaca gcacagcaat tgcctgatta attccccaga
     6361 gtgcattgat gtctgtcaag actggtagtt ctgtctcatc catagaaaaa ggaatgtcaa
     6421 tgtaatgtaa agcatcaaac caatgaacat catgtgctct tatggagtcc agccatgaag
     6481 ctgattttac aaaattaaca ttagacgacg tcttcgattt tgaatgggaa tataaatcac
     6541 acattcgcct ggattgaggt gtcaaattat catatgcgat ctgggctacc agttggtgcc
     6601 ctatcgcgtt ccatgcataa ccagcattag caataaataa aaaaaacaat accaatatgc
     6661 ttcttatcat tcttaactca ggtaagggga ccaagccggc tcttgaacat ctccttcacg
     6721 agcaggaagt ctcattctaa ttctcccgtc aattgacaca atgcctaaaa cccctctgtc
     6781 ctgataacgg gtagcataaa gcactaaacg actatttgga gagactgatg gtgattcatc
     6841 aaggccagaa aaggttaaat tggaaatcgg gccaccacct gtattttgta caccaatatt
     6901 aaattgtcta tcctccctgt gtagcatgac gatatgcttc atatctggag tgtaggaggc
     6961 acgggcatta taattgcctt cgaaagtgac tcgacttatt tggccatcgg ccaatgataa
     7021 gcgatatact tgcggtgacc cgcccctccc tgaagtgaac agcaaactgc gaccatcagg
     7081 agaataacgt ggttccgtat cgatagaatt accaaaagta agctgtttca tagaaccgct
     7141 gctcaaatca acatcgtaaa ttttgggtgt accgcttttg gataatacaa ctgccaaatg
     7201 ctgaccgtca ggtgaccatg cgggcgctcc attaattcct gggaagctcg taattaggcg
     7261 gcgctgtcct gtttcaacgg agacggtaaa aatttcagct tttttctttt caaaagaaac
     7321 ataggatatc gactttccat taggtgacca tgaaggtgac ataataggct ctgaagagac
     7381 gagtaagctt tgaggattgt gcccgtcagc atcagcaacc tccagggagt agcgagacct
     7441 tcctccggct ctttgtaccg aaatataagc tatcctggtg gagaatatcc ccctttctcc
     7501 agtcagtttt tggtatacct catcactaat atgatgtgcc aaagctctta cctgatttgc
     7561 attgatctga aaggtttttg tcaacaaaat attgccattg gctacggcat cggccaaagt
     7621 aaagctcact tcaatccggt taccaacctg attaactcgg ccagtgacta cactgtcagc
     7681 gccaagttga cgtaatgtac tcacggaaga ttgagaattc gcgccttgtg gtccagagat
     7741 aattttaaat tgtcctgata tagtcaaatc attctctatg acatttccta tttcctgagc
     7801 cgccgcgtct gagccaaaag aattgatggc tataggcagg gctgaattaa ttccttgagt
     7861 tagttccaaa tcaagagcaa tcacttgacc ggtaaataac agcaagaaaa gtgaaataat
     7921 tcgattgaac acgagcttta ccccctaact tgttctggac gaacggttaa actgatatcg
     7981 cgaaacaaat taaatgtttc aacgtcttta ggcacgggaa gtggtgaagc cttgtatatt
     8041 gctgtttgtg ctgacctatc taacaaagga tcaccactgc tgcgagttag agacacttcc
     8101 agtacagttc catcaggagc aaggcgtatc ctgaattggc tcgacagaga actgtcgaca
     8161 ttttcaggta aaatccagtt gcgtccgatc gcattgacaa taagtgcctt gtatttatca
     8221 acttcaccag ctattcgtgc ttgtctttca gcattttggg ctgcctgttg ggctgcagcc
     8281 gcttctgctt gtctttttct ttccatctct gctttttctt gctcagcacg ggctttttcc
     8341 gctttcagct tttcggcctt agcctgttca gctttaagtt tctcggcttt ctctttttct
     8401 tcctgttttt tcttattgag ttcagcaagt tttttagctt ccaattctcg ttgtttggcc
     8461 aatttttcgt tttgtttctt taactcttca atacgtttcg cttctaatgc tttttgctca
     8521 gccaattctt ttaaccgttt tttttcttct tcaacttgtt tttttctggc aattgcaatt
     8581 ttattagctt cttctttcaa tcttgcaatt tcttgttgct ctttgattct ttgctgtttt
     8641 gcagcctcag cttgccttaa taactccttt tgtcgattaa tttcagcttt cttctgttgt
     8701 tcacgttctt gtttcaaacg attaacggtt ttcattattt ctttattgtc gacactgacc
     8761 gcttttacta cttcctgctg agaagcagta ggttgcatca tgccaggaga atttttggat
     8821 tccatggtta gcacaggtct tttgctgcta ttatctgtaa aaagcaatac aagtaaaaaa
     8881 agatgcagtg tgacagcaaa aagaaaagct tttctatagg taagatcact tatcatgctt
     8941 gcccctcatc accagtctga gagtcagtaa gcaatcccac ttgctcagct cctgcttgct
     9001 ttaagagtgc catggcttga acaactttcc cataagcaac cccctgatct cccttaacaa
     9061 gcacattgag tttttgacct gactgtttgg ctaactccag ttctgccgca actcgtacta
     9121 ataatgcctg agcttcaata ggttctgctg gcgtactact aatatttaaa aaatagctgc
     9181 ctaattgatt cactgacaca atgataggtt ctctgtcagc agcctgtaaa gtttgactgg
     9241 ctgctttggg cagatctact gtaacacctt gtgtcagcat tggagcagta atcataaaaa
     9301 taaccagtaa aaccaacata acatcaatgt aaggcactac attaatttct gagattggac
     9361 gttcacgatt ggttttagat cgattcattt taagtcttat cctctaggtg aaccagaagt
     9421 ttgttgttca attaaagaaa tcagttcttc ttgaaataaa tcatatttat tcagcaaagt
     9481 attagcacgt gtcgtatatc ggttgtaggc aataaccgca ggaattgcag taaatagccc
     9541 taaagcggta gcaaccagcg cttctgaaat acctggtgcc accattgcga tagtggcttg
     9601 ttgggcatgt cccaaagcct gaaatgatgt cattataccc caaactgtgc caaataaacc
     9661 cacatagggg gaaatagatc ctacagatgc aaaaaatggt aaatgttgct ccagtttttc
     9721 cgcttctttg gcatgactta tctgcattac tctttgtata ggctcaatag atactttgcc
     9781 ttgttttctg cagcgaacaa attctttaaa accagcgtga aagatagcgg ctaccccttg
     9841 tctttcatca ctattactgt ctacatcagc atagagtttg ctcaaatctc cacaatccca
     9901 gaaccttctg gtaaaggttt cagtcatttg ctttctacga ttaaagaacc atgctctttg
     9961 aaagattaat gtccatgaaa ttatcgaagc aattaataat atcaacataa cggactttac
    10021 taccaaaccc gcttgcataa aatacattag tacatttgct tgattagcca ccattaatct
    10081 ccacactgaa aggtttcgtt attcatttct cataatatta ggtagtcgct tgggctttag
    10141 attactgcca acgcaaacta cttgaacact ggccctgcaa attaattgtt tatgttgatt
    10201 agatatctct tgattaaata gaaaactgca cgctctaaat tctttaattt ctgttttaat
    10261 tgttaattga tcatctaacc tggccggaaa tacataatta attgtgagtt cgtgaatcgc
    10321 aaataaaata tcggattttt cgaattcact tagaattaaa ttgttttgcc ttaataattc
    10381 agttcgagct ctttccaaat aacaaaggta gttagcatga taaacaattc ccatataatc
    10441 aacatcttct gcatagactc taatgttatg ctgatgattt gatataagat tcatgtgtta
    10501 ttcctgttcg atcgcactaa agccaaaatg ctgataagct ttcgaagtgg ctattctacc
    10561 gcgaggagtg cgcattagaa agccttgttg tattaaaaat ggctccaaaa catcttctat
    10621 agttcctttt tcttcgccaa tagcggctgc aatactatct atgcctactg gaccaccatt
    10681 gaagtgttct attaccgcta acaataattt tctatccatt aaatcaaacc cgtgctgatc
    10741 cacttcaagc atttccaatg cttgttgggc catatcaaca gtaatgatgc catttccttt
    10801 cacttcagaa taatccctga ctcttcttaa caggcgattg gctattcgtg gtgttcctct
    10861 ggagcgtaat gcaatttctc tggctccttc gggttttgta gggacaccaa gcaaatgcgc
    10921 ggagcgtgcc acaatctggg taagcgaatc aacagaataa tattccaaac gttgcactat
    10981 accaaatcta tcccttaatg gcgaggtaag caaccctgct ctcgtcgttg ctccaattaa
    11041 tgtaaaggga ggtaactcta atttaataga tcgtgctgct ggcccctcgc ctatcatgat
    11101 atccaactta taatcttcca tggctggata aagaatttct tctataacag gacttaatct
    11161 atgaatttca tcaataaata gtacatcatt ttgttgtaga ttcgtcaata tagcagcaat
    11221 atctccagca cgctcgatca ctggcccaga agtttgtcgg atattcactc ccatttcgtg
    11281 agctatgata tttgcaaggg tagttttacc aaggccaggc gggccaaaaa taagaacatg
    11341 atccaaagga tcgtttctct ttcttgccgc attgataaat atctgcatct gtgagctaac
    11401 agaatcttgc cctacgtact cgctcaagct aagcggcctg atggctctat ccattgcgtc
    11461 ttccgagaca atactttgac tacttattag gcgatcactt tcgagcataa gaggttttat
    11521 cgggttcatt cgaatgaaag cattttaaca taggtttatc taatagcaga tctttgtaag
    11581 cttatttatt taataattaa atgcaattga tttcacgatt aaggttgatg attttatatg
    11641 atatcaaaat gatattataa agtgtgattc tcacttacaa tatccatcag ctgttttcga
    11701 gctctaaata tacgtgagcg tacagttccg atggggcaat gcatttcttt tgcaatatcc
    11761 tgataagttt gtccatcaaa tatgtgcagc ccataacata atcgtaactc ttcagataaa
    11821 catgacattg ctgcttctat ttgtttctca aactctaatc ccattatgtc tgactctgga
    11881 gaattatgtg gtgtagaaaa ttgttcatcc atatactgca tttctgaatc agcatataaa
    11941 ttattggtac gaaaataatt tttaatagta ttttgagtaa ttttatatag ccaggttgaa
    12001 aactgacttc gttcattaaa ataaggtaaa tgcctaaata ctttgataag aacttcctga
    12061 gttaaatcat tgacatttgt ccagtcatgc gtgtaaaaat aaatcattcg tctaattttg
    12121 ccctgataac gctcaaacag tttatcaaaa gctaaaccgt tgcctttttg agctaattca
    12181 attagagatt catcacttaa agaggtctca atatgcatag cttcctcatt attcctttgc
    12241 ttgttattac gttaggataa gata
//
LOCUS       DBNCWK010000095        11903 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.095, whole genome shotgun sequence.
ACCESSION   DBNCWK010000095 DBNCWK010000000
VERSION     DBNCWK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11903
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.095"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            105..1235
                     /locus_tag="AC06IO_002136"
     CDS             105..1235
                     /locus_tag="AC06IO_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIO9875312.1"
                     /translation="MRGFLLALLLAINTVLWADELVGFAAFENGDYTTAYPHLMQAAK
                     EGNEEAMYLLGRMYQYGYGVTTNYEEARNWYQKAADKNNALAQLSLGFMYDTGKGVSQ
                     DFAEAFKWYMKAAEQGNPIAQRNIGLMYATGDGVAASDDKAFIWFKKAAEQGYSKAQV
                     NLGYQYMMGKGTPKDVKKAFEWYQKAAEQGDEKGEYSLGLLYTGQEGGVSADDKAAFY
                     WFSQAANHGHVNAQTYLAYYYLKGYGVDADPVKAAYWYQSAAEKGQPEAQAQLGQLLL
                     TGTGVDKDYQQAAYWFGKSAHQGNPVGQAKLGYMYLAGLGVNKSLVKAYAWLKIAAEN
                     KNEEAAKQLKSLEAKLTEQEKLEAEKMIKDLGPLESKENYED"
     gene            complement(1371..3749)
                     /locus_tag="AC06IO_002137"
     CDS             complement(1371..3749)
                     /locus_tag="AC06IO_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIO9875313.1"
                     /translation="MINIIKNRNKKNHTASTELKSEIEKEQVKLLFDQVGMAISGEAL
                     AVTILPIALWSVTNHELLIIWMIFTYVFSDLYKSMLLFYYNKQPTLFSTEKWLFLFNL
                     GVVSSGLAWGFASSIMIPASSTLHQIFVVFLITGVTAAANSLYSPVRLSYFLFLVTAF
                     VPFTIWLFSKGQVYLLLGFCGIIYMGIMLFISYYSNKILINTLKMRFKISNLNSTKDI
                     LEKKVVDRTKELEKILALTKSTLESTADGILVVDLKGNIEFCNQQFLKMWGISPGFIE
                     SHNDTETIQYVLNQLKNPEEFLKQIKELYNNPKQESFDELHFKDGKIFERYSQPHWLD
                     NKIIGRVWSFRDVTLRTRLAYQANHDSLTGLPNRTALYDRLDHAINYCKRLNTSLSVL
                     FLDIDNFKLINDSLGHDAGDILLYEFSRRLKGCIRHSDTVARFGGDEFVLLLMTTEPK
                     DVILVAQNILKAVAQPIKLPTQEVIVTTSIGISLFPKDGIDASTLLKNSDTAMYLAKK
                     EGRNNFQFYNNSINQQSLKRLEIQTQIHNAIKNNEFFILYQPIYNLRNGELVGAEALI
                     RWGHPAIGLLSPDEFIPIAEETGLINQVGEWVLRNACIQNKTWQLMGLPHISISVNVS
                     WRQLKNSNFIEIAESVLKESRLDPQYLEIEFTESTMIQKSTPIDSTLIKISQLGIKMA
                     IDDFGTGYSNLSYLKTFPFNKLKIDRSFIQNCTTNPNDASIVEAIIAMAHSLELKVLA
                     EGVDTIDKAFFMHQHGCDEVQGFLYGLPVKADKFVKLMQKTKIRHSKFINKT"
     gene            complement(4064..5032)
                     /gene="lspK"
                     /locus_tag="AC06IO_002138"
                     /gene_synonym="gspK"
     CDS             complement(4064..5032)
                     /gene="lspK"
                     /locus_tag="AC06IO_002138"
                     /gene_synonym="gspK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspK family T2SS minor pseudopilin variant LspK"
                     /protein_id="HIO9875314.1"
                     /translation="MFMSSPPSRSIASSKMQGSALLTALFIMTLVAIVATAMSTRLQQ
                     DIYRTRLVIAQDKLYLASQAVTFWALNELLDKNNRFTKTNQLGMVAQYPKNMESIYNQ
                     VQLSGGIFDLQARFNLNNLIEKKSIPTLMHLISHTYSKATGQERANIALGVKHWLLAY
                     DLGRGEDIYTSYYLSQKPPYYPSHQLIKSKSEFRLIKDVSAPAYIALEPFITALPEST
                     SININTAPKQILMSLGDGLSDAQANELITARGEDGITDLKEINELLKKFNIPSDQITI
                     ESQYYLSVAYAKNDEFSLVVYSLLKRSRDRKGKLLTSVIRESINNF"
     gene            complement(5019..5636)
                     /gene="lspJ"
                     /locus_tag="AC06IO_002139"
                     /gene_synonym="gspJ"
     CDS             complement(5019..5636)
                     /gene="lspJ"
                     /locus_tag="AC06IO_002139"
                     /gene_synonym="gspJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspJ family T2SS minor pseudopilin variant LspJ"
                     /protein_id="HIO9875315.1"
                     /translation="MIKNKGFTLIEILIALTVFAILATITSSTLYYAFNTRTRVNAQA
                     ERLNALQLAISIIQQDTSQTVERAIRGNEMRLFPIFVGQPQYLELTRDGVVNPNSAEK
                     RSTLERVALVCQDSKLLRRTWNTLDPIDRNIYKDKVLLDNLSECHFNYLNQNLQLLSE
                     WREQAVNQNQQREPLPKAIQMNLTLKDWGDMNMLFIIPGALYVYE"
     gene            complement(5633..6010)
                     /gene="lspI"
                     /locus_tag="AC06IO_002140"
                     /gene_synonym="gspI"
     CDS             complement(5633..6010)
                     /gene="lspI"
                     /locus_tag="AC06IO_002140"
                     /gene_synonym="gspI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspI family T2SS minor pseudopilin variant LspI"
                     /protein_id="HIO9875316.1"
                     /translation="MMPKTKLVSGFTLIEVLLALTVIAIALTALLKATAQNIDNTHRI
                     KEKTISHWVAMQGIAMIQLNLLRTSQSQESTQATTILGQKWYWRAKISPTPIKRMQQI
                     TISVSSKQAGPFREELIAFRYLP"
     gene            complement(6007..6492)
                     /gene="lspH"
                     /locus_tag="AC06IO_002141"
                     /gene_synonym="gspH"
     CDS             complement(6007..6492)
                     /gene="lspH"
                     /locus_tag="AC06IO_002141"
                     /gene_synonym="gspH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH family T2SS minor pseudopilin variant LspH"
                     /protein_id="HIO9875317.1"
                     /translation="MTTHKGFTLIEILIVIVIIGITLGFALIAFGDFGESRRIQFSAE
                     QLANTLSLAQQQAILESSTLGLKIDNSSYQILKFQDRAGWNPISNKGIFKLYYFPKNV
                     VISLKTNLKHSRGDPAIIIAPSGDMTPFTIEFGTDKEKWIAKLTGSHNGDLDFTMTKS
                     Q"
     gene            complement(6479..6901)
                     /gene="lspG"
                     /locus_tag="AC06IO_002142"
                     /gene_synonym="gspG"
     CDS             complement(6479..6901)
                     /gene="lspG"
                     /locus_tag="AC06IO_002142"
                     /gene_synonym="gspG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspG family T2SS major pseudopilin variant LspG"
                     /protein_id="HIO9875318.1"
                     /translation="MNRQKGFSLIEIMVVVVILGILASIVVPKIMGRPDEARKVKAKQ
                     DVLAIQNALDLYKLDNGSYPTTDQGLMALVEKPTSNPVPTNWKQYLKSLPKDPWGREY
                     LYLNPGQHTDVDVFTYGAAGQPGGTGINAEIGNWDDHS"
     gene            complement(7005..8204)
                     /gene="lspF"
                     /locus_tag="AC06IO_002143"
                     /gene_synonym="gspF"
     CDS             complement(7005..8204)
                     /gene="lspF"
                     /locus_tag="AC06IO_002143"
                     /gene_synonym="gspF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspF family T2SS innner membrane protein variant
                     LspF"
                     /protein_id="HIO9875319.1"
                     /translation="MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQT
                     LTQQRAASSKGKVSAADLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGV
                     RSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
                     VQQALIYPLLMIIVSTAIICFLLTFVVPKIIDVFTESGQTLPPMTQLLINMSQFIKSY
                     GVYSLVTIIVALIGFKKSLSNIKIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILF
                     AAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGGGISEALKETKFISPMAIHLIA
                     SGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIF
                     SMEQLVA"
     gene            8301..9710
                     /gene="glnA"
                     /locus_tag="AC06IO_002144"
     CDS             8301..9710
                     /gene="glnA"
                     /locus_tag="AC06IO_002144"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIO9875320.1"
                     /translation="MTKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFL
                     ENGKMIDGSSFKGWQKIHQSDLALVPDLTSIKPDPFFQDNTLFIRCNVVDPQTMMGYD
                     RCPRSIAQRAENYLQSTGIADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWN
                     SGKEMEGGNIGHRPPLKGGYFPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATAN
                     QCEVATRFNTLTKKADELQILKYVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLY
                     KDGYNLFSGDQYSGLSETALYYIGGIIKHARALNAFTNPSTNSYKRLVPGFEAPVLLA
                     YSARNRSAAIRIPHVSNPKARRIEVRFPDPTANPYLAFSAMMMAGIDGIQKKIYPGQP
                     MDKDLYDLPPEELVDVPTVCSSLEMAMDHLRADHEFLLQGDVFSRDFINNYIQMKEEE
                     ITKIRSLTHPYEFELYYSL"
     gene            9723..10283
                     /locus_tag="AC06IO_002145"
     CDS             9723..10283
                     /locus_tag="AC06IO_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIO9875321.1"
                     /translation="MNKLPLFVFLMMVICLSSAQIYKWTDSQGVVHFSDKPHQGAEKI
                     KIPEAQTYTPPANETGGSSEQEQKSVKQEDQEKYTKLEIIQPQNEATIRNNQGYVVVA
                     VQIEPDLSPGDNLQLIFDGAPLGEPQPNLIFQLNGIYRGSHTIAVQVLNDKGDVLKVS
                     DSITIFMHNPRVGMGKGRKKNINSNK"
     gene            10296..11069
                     /gene="yaaA"
                     /locus_tag="AC06IO_002146"
     CDS             10296..11069
                     /gene="yaaA"
                     /locus_tag="AC06IO_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIO9875322.1"
                     /translation="MLTLLSPAKKLLSISKHYSKKTSNPLLLDKALQLVKIMKLKSVE
                     QIADLMDLSRQLAELNYERYQNFDLKNNPMNHSYPALFLFQGDVYQGLNANSWKDEEI
                     EYAQSHLGILSGLYGFLRPLDRIQPYRLEMGVNLENPAGKNLYAFWSKIVTNILNQIL
                     AGQSNPVLINLASTEYFKVVDEKKLSYPLVTINFYEQKNSELKMIGILAKKARGMMAK
                     YIMQNRIDSIEQIKEFSESGYLFNKEISSPNSLNFIRIH"
     gene            11089..>11903
                     /locus_tag="AC06IO_002147"
     CDS             11089..>11903
                     /locus_tag="AC06IO_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIO9875323.1"
                     /translation="MWNLSSRVHALNHFPKQGIECYVKRDDELGCGISGTKIRKYSSL
                     IPFLKINGIRHLIIIAGAQSNNLLAALQVARECQLKVTAFLVKPKHLKIQGNFKLSLL
                     FLHENEIIWINREEWYRVNEFAEQYLEGLRETAYILSEGASVKESMKGAMSLASDIKE
                     NEKILGFAFDHIFVDAGTGFSAIALIKGFYELQHKGFIHVLLLADSEEVFKKKLMHWA
                     GVNPDNYACFYPTTAKSFGSVNQTIKQEIKRLAYEEGILADPIYSAKLFYEAR"
BASE COUNT         3737 a         2062 c         2362 g         3742 t
ORIGIN      
        1 gattgagatt tttttaaatt tttccatgcg ttaaccgaac aattacttgc tttgttgttt
       61 ctttattcta cgcttaaatt aaaaggaaat aaaatggagc aagtatgcgc ggttttttgt
      121 tagctctatt actggcgatt aatactgttc tttgggctga tgagttagtt ggttttgcag
      181 catttgaaaa tggcgactat accacagcct acccacattt aatgcaggct gccaaagagg
      241 gcaatgagga agctatgtat ttattgggac gcatgtatca atatggatat ggggtgacaa
      301 ctaattacga agaagccaga aattggtatc aaaaagcagc tgataaaaat aatgcattgg
      361 ctcaattaag cttgggattt atgtacgata caggtaaggg tgtatcacag gattttgcag
      421 aagctttcaa atggtacatg aaggcagcag agcaagggaa tcccattgca caaagaaata
      481 taggattaat gtatgctaca ggagatggtg tagctgccag cgatgacaaa gcatttatct
      541 ggtttaaaaa ggcggcagaa cagggctata gcaaggcaca agtgaatttg ggctatcaat
      601 acatgatggg taaaggaact ccaaaagatg taaaaaaagc atttgaatgg tatcaaaaag
      661 ctgcagagca gggagatgaa aagggtgagt acagtctggg tttactgtat acaggacagg
      721 aaggtggagt tagtgcggat gataaagctg ccttttactg gttttctcaa gcagccaatc
      781 atgggcatgt caatgcacaa acttatttgg cttactacta ccttaaaggg tatggcgtag
      841 atgctgatcc tgtgaaagca gcttattggt atcaatctgc agcagaaaaa gggcaaccag
      901 aagcacaagc ccaattagga caattgttat tgacgggtac aggtgttgat aaagattatc
      961 aacaggcagc ttattggttt ggaaagtcag cacatcaggg aaatcctgta ggtcaagcca
     1021 agctggggta tatgtattta gcaggattag gagtcaacaa gagtttggtt aaagcttatg
     1081 cctggttaaa aattgctgct gaaaataaaa atgaggaagc tgcaaagcaa cttaaatctt
     1141 tggaagccaa gttgactgaa caagaaaaat tggaagcaga gaaaatgatc aaggatttgg
     1201 gaccgttaga aagcaaagag aattatgagg actaaagtta aaaactgaat ccagaagatt
     1261 atagttgtag cttgataatt tgttgtgtgt ttaaaatact ggtttaaatg agctagttca
     1321 attgatgagt agtctgtatt ttctttatag taacaaaaaa aacaacaacc ttatgtttta
     1381 ttaataaatt ttgaatggcg gattttagtt ttttgcatta atttcacaaa cttatcagct
     1441 ttaacaggta ggccataaag gaaaccttgt acttcatcac agccatgttg atgcataaaa
     1501 aatgctttat caatcgtgtc aactccttct gccaatactt tgagttccag gctatgggcc
     1561 atggcaataa ttgcttcaac tatggaggca tcatttgggt tagtggtgca gttttgaata
     1621 aatgaacgat caattttaag tttattgaag gggaaagtct ttaaataact taaatttgag
     1681 taaccggtac caaaatcatc aattgccatt ttaatgccca actgggaaat ttttattaac
     1741 gtcgaatcaa tgggcgttga tttttgtatc atagtacttt cggtgaattc aatttccaaa
     1801 tattgaggat ctagtctgga ttcttttaat acagattctg caatctcgat aaaattgcta
     1861 ttttttagct gtctccatga gacattaaca gaaatactaa tatgcggtaa ccccataagc
     1921 tgccaggttt tattttgaat acaggcattt cttaataccc actcgccaac ttggttaatc
     1981 aagccggttt cttcagcaat aggaataaat tcatccggag agaggagtcc tattgcagga
     2041 tgcccccaac gtatcagggc ttcggcacct actaattcac cgttacgtaa attataaatg
     2101 ggctggtaaa gaataaaaaa ttcattattt ttaattgcat tatgtatctg tgtttgtatc
     2161 tcaaggcgtt tgagcgattg ttgattgatc gaattattat aaaattggaa attattgcgc
     2221 ccttcctttt tagccaggta cattgcggta tcagaatttt tgagtaaggt gcttgcatca
     2281 atgccatctt tagggaaaag gctaattcct atacttgttg tcactatgac ttcctgggta
     2341 ggcaatttga taggttgcgc tacagccttt aaaatgtttt gcgctactaa aataacatct
     2401 ttaggttcag tggtcatcaa taacaaaaca aactcatcac cgccaaatcg agcaactgta
     2461 tcgctatgac gtatgcatcc tttaagacgc ctggaaaatt catacaataa tatatcaccg
     2521 gcatcatggc ccaggctgtc attaattaac ttaaaattat caatatccag aaataacact
     2581 gataatgagg tgttcaatcg tttacagtaa ttaatcgcat gatccaggcg atcgtataga
     2641 gctgtacgat tgggtaaacc agttagagaa tcatgattgg cttgataggc taatctggtt
     2701 cttaatgtca catctctaaa actccaaaca cgccctataa ttttattgtc taaccaatgt
     2761 ggttgtgagt atctctcaaa gattttcccg tctttgaaat gcaattcatc aaagctttcc
     2821 tgctttggat tattgtataa ttctttaatt tgttttaaaa attcttccgg atttttaagc
     2881 tgattcaata catattgaat tgtttctgtg tcattatgac tttcaataaa ccctggagaa
     2941 attccccaca tcttgagaaa ttgctgattg cagaattcaa tattaccttt gagatcaaca
     3001 actaaaatac catccgctgt tgactctaaa gttgatttgg tcaaagccag gattttttcc
     3061 aattctttgg ttctgtccac tacttttttt tctaaaatat ctttggttga attcaaatta
     3121 gagattttaa agcgcatttt taaggtatta atcaaaatct tgtttgagta atatgaaata
     3181 aacaacataa tccccatata aataatgccg caaaacccta acaataaata aacttgtcct
     3241 tttgaaaata accatatagt gaaaggaaca aaagcagtga ccagaaataa aaaataggat
     3301 aatcgcacag gtgaatagag tgaattggct gctgctgtaa ctcctgtaat aagaaaaacg
     3361 acaaagattt gatgcaatgt acttgaggct ggaatcataa ttgaactggc aaatccccat
     3421 gccaagccag agcttacaac acccaagtta aaaaggaaca accatttttc ggttgaaaat
     3481 aatgttggtt gtttattgta atagaagagc agcatgcttt tataaagatc tgaaaaaaca
     3541 taagtaaaaa tcatccaaat aataagcagc tcatgatttg taacagacca taatgcaata
     3601 ggtagtatag ttactgctaa agcttcacct gatatagcca taccaacttg atcaaaaagc
     3661 agtttgacct gctccttctc aatttctgat ttcaactcag tggaagcagt atgatttttc
     3721 ttgtttctgt ttttaataat attaatcata acaagtagtt agtaaaatgg tatgttaaaa
     3781 agcgaagcga gcattaatgt ggattgtttt cctcgtaaac agttagaaaa taaaactaag
     3841 tgcatcgtga aatccaaatg aatttgatga tatctattag tatagtaaat gatcagatta
     3901 tattaaaata tatcgattgg tggaggatga aatctcagca agaattatat tttccagaaa
     3961 ctaaaagcac tcattcctgg taaaaataaa aatcggacgg caacccatta actaaaagtt
     4021 ggattctggc gtattcatat tgtttggaac atttgatctt aagtcaaaaa ttattaatac
     4081 tctctctaat gacgcttgtt aataacttac ccttcctgtc acgactacgt tttaataaag
     4141 aatacacaac taaactaaat tcatcattct ttgcataggc aacgctgaga taatattgac
     4201 tttcaatggt tatttggtca ctgggaatat tgaatttttt taataactcg ttgatctctt
     4261 tcagatcagt gataccatct tctccccgag cagttattaa ttcatttgcc tgggcgtcac
     4321 ttaatccatc tcctaatgac atcaatattt gttttggggc agtattaatg ttaatgctcg
     4381 ttgattcagg cagggctgtg ataaaaggtt caagggcaat ataagcgggt gcacttacat
     4441 cttttatcaa tcgaaattca gatttacttt ttataagttg atggctggga tagtaaggag
     4501 gtttctgaga gagataataa gaggtataaa tatcttctcc tcggccaaga tcataagcaa
     4561 gcaaccaatg ctttactccc aaggctatat tagcacgttc ttggccggtt gcctttgagt
     4621 aggtgtggct tatcaggtgc attaaagtgg gaatagattt tttctcaatc aggttattga
     4681 ggttaaatcg tgcttgtaaa tcaaaaattc caccacttaa ttgaacttga ttatatatgg
     4741 attccatatt tttagggtat tgggctacca tccctaactg gttggttttt gtaaagcggt
     4801 tgttcttatc aagtaattca ttgagcgccc agaaagtaac tgcctgagaa gcaagatata
     4861 gtttatcctg agcaattact agtcttgttc tgtaaatgtc ctgttgtaat cgtgtgctca
     4921 tggcagtggc aactatagca accaatgtca taataaatag agccgttaaa agagcgcttc
     4981 cttgcatttt agaactggca atggatcttg aaggtgggct actcataaac atagagtgct
     5041 ccgggtatga taaaaagcat gttcatatct ccccagtcct ttaaagtgag attcatttgg
     5101 attgctttgg gaagaggttc tctttgctga ttttgattga ctgcctgttc tcgccactcg
     5161 gagagaagct gtaaattttg atttaaatag ttgaaatgac attcagacaa attatccaga
     5221 agtactttgt ctttatagat atttctgtct ataggatcta aagtattcca tgtgcggcgc
     5281 agtaatttgc tatcctggca aacaagcgcg actcgttcca aggtactgcg tttttcagca
     5341 ctattggggt ttactacccc atccctggtt agttcaaggt attggggttg accaacaaat
     5401 attggaaaca ggcgcatttc attacctctt atagcacgtt ccactgtttg ggaagtatct
     5461 tgttgaatga tacttattgc cagctgcagt gcatttaagc gttcagcctg cgcgttaact
     5521 cgggttcgag tattgaatgc gtagtataag gttgaggagg taatagttgc taatatagca
     5581 aaaacagtaa gagcaattaa tatctcaatc agtgtaaatc ctttattttt aatcatggca
     5641 agtacctgaa tgcaatgagt tcttctcgaa agggacctgc ttgtttggaa ctgactgaaa
     5701 tggttatctg ttgcattcgt tttatagggg tcgggctaat tttagctctc cagtaccact
     5761 tttgtcccag tatggtggtt gcttgcgttg attcttggct ttgagaagtg cgcagcagat
     5821 ttaattggat catcgctatc ccttgcattg ctacccaatg acttattgtt ttttctttaa
     5881 ttctatgcgt gttgtctata ttttgagcgg ttgcttttaa aagcgctgtt aaagcaattg
     5941 caatgacagt aagagccaat aagacttcta tcaaagtaaa tccggatact agtttagttt
     6001 tcggcatcat tgagattttg tcatggtaaa atccaaatca ccattatggc tgcctgtaag
     6061 tttggctatc catttttcct tatctgtgcc aaattctata gtaaaagggg tcatgtcacc
     6121 agatggcgct attatgattg cagggtcccc ccggctatgt tttagatttg ttttaaggct
     6181 aatcacaacg tttttgggaa aatagtagag tttaaaaatt cctttgttcg atatgggatt
     6241 ccatcccgcc cggtcctgaa acttaagaat ttggtaactg ctattatcta tttttaaccc
     6301 aagggtactg ctttctaaaa tggcttgttg ttgagccagg cttagggtat ttgccaattg
     6361 ttcagctgaa aattgtatac gtctgctttc cccgaaatca ccaaaagcga ttaaggcaaa
     6421 ccccaaggtt atacctataa tcactatgac aataagaatt tctatcaggg taaaaccctt
     6481 atgagtggtc atcccagtta ccaatctccg cattaatccc tgttccgcca ggttgtcctg
     6541 ctgctccata agtaaagaca tcaacgtcag tatgttgtcc tgggtttaaa taaagatatt
     6601 ctctgcccca tggatctttt ggcaatgatt tcagatattg tttccaattc gttggtacag
     6661 gattgcttgt tggtttttct accagagcca ttaaaccttg atcagttgta gggtatgaac
     6721 cattgtcaag tttgtataaa tcgagtgcat tttgaatggc taaaacatct tgtttcgctt
     6781 tgactttacg ggcttcatcc ggacgcccca tgatcttggg gacaactata gatgccaata
     6841 tgcctaaaat cacgacaaca accataattt cgatcaaaga aaagcctttt tgtcgattca
     6901 tttgtgtacc tcttgaaact aaatgttaaa ttataacaca aatattgatg cctatgaata
     6961 gacttaaatt ttaaaagggt gcggataacc cttcctctgc ttaatcaggc aactaattgt
     7021 tccattgaaa aaataggtaa taaggtcgca agtacaataa acaaaacaac agcacccatt
     7081 aatagaataa ccataggttc taataaagtt aaggatgttt caatcagtcg gtttacttca
     7141 ttatctaaat gagacgccga gcgttccatc atgtctgaaa gttggccgct tttctcacca
     7201 ctggctatga gatggatagc catagggcta atgaattttg tttctttgag ggcttcactg
     7261 attccacctc cttcacggac tcgtagagta gctaaatcaa aagcctgtct catcacgata
     7321 ttggttacca aacttgaaga aacacgcatg gtttcaagta cactgactcc cgctgcaaat
     7381 agaattccaa acgtgtgaat atatcgtgct acattaattg ttttaaccaa ataagacacg
     7441 ataggtagtt taagcatgaa ttgatgccat gctgttttaa tttttatatt actgaggctt
     7501 tttttaaatc caatcagagc cacaattatt gtcactagac tatacacacc ataagatttg
     7561 ataaattggc tcatgttaat aagcaattgc gtcattggtg gtagtgtttg gccactttcg
     7621 gtaaacacat caataatttt agggacgaca aaagtaagta aaaaacagat aatggcagta
     7681 gaaacaatga tcatgagcaa tgggtaaatc aaggcttgct gcactttttg tcgagtttgt
     7741 tgttgttttt cagtataatc agctaatttt tctaaaacga catcaagacg ccctgtctgc
     7801 tcgcctgagc caactgttgc ccggtatagt tctggaaagg cattgggata ttgagccatt
     7861 gcctgagcaa gaccataccc ttccaatact ttagagcgaa ctccgataat aagctctctg
     7921 actttgtctt tttctgtttg ttcgctgacc ccgcgcaatg attcttcaac aggaataccg
     7981 gcagccagaa gtgttgccag ctgtctcgtg agtaatgcca aatcagccgc agaaacttta
     8041 cctttgctgc tggcagcacg ttgctgggtt aatgtttgga cttgagtagg gattagtcct
     8101 tgttctcgca ataattgacg tgcatgacgt tcagaatcag cttctattac acctttgcta
     8161 atgctgccat tcacttttag cgcttgatat tggtaggcgc ccatattatt tttgttaaaa
     8221 ttaaaactat agagtgtaat ctattgggat taataagtca cttaaggtaa actatacgcg
     8281 gactatcctg gagacaaaag atgacaaaaa atgtagtgct tgatgcaatc aaagaacatg
     8341 acgccaaatt tgtggattta agatttacag atatccgtgg aaaagagcaa cacattacca
     8401 tacctgtttc agcagttgat gatgatttcc ttgagaatgg aaaaatgatc gatggctcat
     8461 cttttaaagg ttggcagaaa attcatcaat cagatttggc tttagtgcca gatttaactt
     8521 caattaagcc ggatccattt tttcaggaca acactttatt tatacgatgt aatgtagttg
     8581 atccacagac tatgatgggt tatgacagat gcccacgttc aatcgcacaa cgcgcagaaa
     8641 attatttaca atctacaggg attgcagatg gtgtaaattt tggccctgag cctgagtttt
     8701 ttctcttcga caatgtgcaa tgggaaacta ctatcagcgg ttcattttat aaaatagatt
     8761 cagacgaggc acattggaac tccggtaagg aaatggaagg cggaaatatt ggccatagac
     8821 ctccgttgaa aggcggttat ttccctgttc ctccagtcga ttcatctcat gatattcgtt
     8881 cagcaatttg tatcacattg gaatctttag gaatggttgt ggaagcacat catcatgaag
     8941 tagctacagc taaccagtgt gaagtagcta cccgttttaa tacactcacc aaaaaggcag
     9001 acgaactgca aattctgaaa tacgtgatac ataatgttgc ccataactat gggaaaactg
     9061 ctacttttat gccaaaacct ttggtaggag ataatggaag cgggatgcac tgtcatcaat
     9121 cactttataa agatgggtat aatctgtttt ccggtgatca gtattcgggg ctttctgaaa
     9181 cagctttata ttacataggt ggaataatca agcatgccag agctttaaac gcttttacca
     9241 atccgtcaac caatagctat aagagattag ttcccgggtt tgaagcgcct gtattactgg
     9301 cttactcagc aagaaatcgc tcagccgcta tccgtatccc acacgttagc aatccaaagg
     9361 cacgacgtat agaagtacgt ttccctgatc caacagctaa tccttatctt gctttctcag
     9421 caatgatgat ggctggcata gatggcattc agaaaaaaat ctaccctggg cagccaatgg
     9481 ataaagatct ttatgattta ccgcctgaag agcttgttga tgtacccact gtttgctctt
     9541 cattagagat ggctatggac catttgcggg cagatcatga atttttgtta caaggagatg
     9601 tgttcagcag agactttatt aataattata ttcaaatgaa agaagaggaa attactaaaa
     9661 tcaggagctt gactcatcct tatgaatttg agttatatta tagtctctaa gggtctatat
     9721 tcatgaataa actgccacta ttcgtctttt tgatgatggt aatttgcctt tccagcgcgc
     9781 aaatttacaa atggacggat agtcagggtg ttgttcattt tagcgacaaa cctcatcagg
     9841 gtgctgagaa aataaaaatt cctgaagctc aaacctatac accacctgca aatgaaacag
     9901 gtggttcttc tgagcaagaa caaaagagtg tcaaacagga agatcaggaa aaatatacca
     9961 aattagaaat tattcaaccg caaaatgaag caacaattcg taataatcaa ggttatgttg
    10021 ttgttgcagt ccaaatagaa cctgatttgt ctccagggga taatttgcag cttatttttg
    10081 atggggcgcc tttaggagag ccacagccca atttgatttt tcaattgaat ggtatctatc
    10141 gaggatctca tacaattgct gttcaggttc ttaatgataa aggggatgtg cttaaagtca
    10201 gcgacagtat tacaattttc atgcacaatc ctcgcgtcgg tatgggcaag ggcagaaaga
    10261 aaaatatcaa ttctaataaa tagaacaggt tatgtatgtt aactttatta tcgccagcta
    10321 agaaactatt atccatatca aaacattact ctaaaaaaac cagtaatcct ttattgctcg
    10381 ataaagcgct acaattggtt aaaataatga agttaaaatc tgtggaacaa attgctgatt
    10441 taatggattt atcaagacaa ctcgctgaat taaactatga gagatatcaa aattttgatc
    10501 ttaaaaacaa cccaatgaat cactcttatc ccgctttgtt tttatttcag ggagatgtgt
    10561 accaaggatt gaatgctaat tcatggaaag atgaagagat cgaatacgct caatcccatt
    10621 taggaatttt gtcaggtcta tatgggtttt taaggccttt agataggatt caaccttaca
    10681 gattggagat gggagttaat ttagaaaatc cggcaggtaa gaatctctat gcattttgga
    10741 gcaaaattgt tactaatatt ttaaaccaaa tactagcagg gcagagtaat cctgtgctta
    10801 ttaacctggc ttcgactgag tattttaaag tcgtggatga gaaaaaatta agttatcctc
    10861 ttgtgactat caatttttat gaacagaaaa atagtgaatt aaaaatgata ggtatccttg
    10921 ctaaaaaagc aagaggaatg atggcaaagt atataatgca gaatagaata gactccatag
    10981 aacaaattaa agagttttct gagtcgggtt atttatttaa taaagaaatt tccagtccaa
    11041 atagtttgaa ttttattcga atccattaaa ttatttaaaa ggtatttgat gtggaatttg
    11101 tccagtcggg tgcatgccct taatcatttc cccaagcaag gtatagagtg ttatgttaag
    11161 agagatgatg aattaggttg cggcataagt ggtacaaaga taaggaaata cagcagttta
    11221 atcccgtttc taaaaatcaa cggaattcga catctgatta ttattgcggg tgctcaatct
    11281 aataatttat tggctgctct gcaggtggct agagaatgtc aattgaaagt gacagctttc
    11341 ctagtaaaac ccaaacattt aaaaattcag ggtaatttta aattaagcct tctttttctt
    11401 catgagaatg aaattatctg gataaatcga gaagaatggt atcgagtcaa tgaattcgct
    11461 gagcagtatc ttgaggggct cagagaaact gcttatattt tatctgaagg tgccagtgtt
    11521 aaagagtcca tgaagggggc aatgtctcta gccagtgata tcaaggaaaa tgaaaagatt
    11581 ttgggatttg cttttgatca tatctttgtt gatgcaggta caggtttttc agcaatcgca
    11641 ctaattaaag gtttttatga attacagcat aaaggattta ttcatgtgtt attgttagca
    11701 gatagtgagg aggtttttaa gaaaaaactt atgcattggg caggagtaaa ccctgataat
    11761 tacgcttgtt tttacccaac tactgcgaaa tcgtttggat ctgtaaatca aacaattaaa
    11821 caagaaatta aacgattggc ttatgaagaa ggcattttgg ctgatccgat ttactctgca
    11881 aaattattct atgaagccag aaa
//
LOCUS       DBNCWK010000096        11875 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.096, whole genome shotgun sequence.
ACCESSION   DBNCWK010000096 DBNCWK010000000
VERSION     DBNCWK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11875
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.096"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..876
                     /locus_tag="AC06IO_002148"
     CDS             <1..876
                     /locus_tag="AC06IO_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0008 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875324.1"
                     /translation="AIALRLAETTPAQDRSRVSQNINNIFPQSLFAAKVGHAFAVRRD
                     IERLLLGERPDQFFSSREFKIDSCIEFASLFNVINDKESSIAGKLALRTPAENRTDVV
                     MKIKGFCAEDSELAIKVQSAFALRRDIERDLLGDNPEQFFNSRDFSVDLCLEFAILFP
                     ELLKGHEKAIGEKLAKLDAKVRSDISRKLEMINGAAHEQSSPFRIIASAMVPKEEPCL
                     TKPTPVVLPSTEEIIPKQESPTSLRNRNVLFKEQTEEHKKKNSQMEPIVSDSEEEEEE
                     EKQNCWTSFCGLFGK"
     gene            1417..2262
                     /locus_tag="AC06IO_002149"
     CDS             1417..2262
                     /locus_tag="AC06IO_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HIO9875325.1"
                     /translation="MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQ
                     ELTLSPYFCTRSDVDPAPYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVA
                     FNPQGELIAVTRKQHIPSGEKYHENFYFKPGDSNYPVHTIAGHHLGLPTCYDQWFPEL
                     SRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCED
                     DLSFYGSSFISTPMGEILVQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHE
                     LVKSR"
     gene            2282..4174
                     /gene="speA"
                     /locus_tag="AC06IO_002150"
     CDS             2282..4174
                     /gene="speA"
                     /locus_tag="AC06IO_002150"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HIO9875326.1"
                     /translation="MNNNTLSDDNLYNIENWGEGYFRINSKGNIEISKKPGKPGVELQ
                     TIVDAADRAGLHLPLLIRCSDILHDRVHRIYQAFTQAREDTYYAGNYQLVYPIKVNQE
                     QSVVRELLKSPDNQIGLEAGSKPELMAVIGMLGDHQSTIVCNGYKDTSYIRTALIAQQ
                     MGHKVFIVIEKKSELDIILKESARLQIKPTLGVRIRLVSKSAGKWENTGGVKSKFGLN
                     AKQVLELILQLKANDMLDCLQLMHCHLGSQIANIHDIRHCMQEVARYYIELRQLNAPI
                     GTIDVGGGLSVDYEGTRSNKGCSMNYSLVEYATHIMLALKHLCQEAEISEPDIISESG
                     RALTAHHAVLISNITDVEIINKAPVLPEISEDDSHVIRDIYDTYQAITDSSPTEIYNY
                     AAHSLNEAHSLFKHGVISLQEKAKVEAFYTNICMEIKDKLDEENPSEKALLMRINECM
                     AAKIFCNISFFQSIPDAWAIGQIFPVAPISQLNRTLSMHSVLQDLTCDSDGTIKQYPG
                     SSCVTTTLMLPSYDINNPYNIGFFLVGAYQEILGNLHNLFGDTNSLDVKLSDDGQFEL
                     DDLVSGDTVTNVLNLAHFDTKKLIQSYEKQLIKSELPKEKALSYLNELRSIFSQLTYL
                     DGNIAQ"
     gene            4177..5217
                     /locus_tag="AC06IO_002151"
     CDS             4177..5217
                     /locus_tag="AC06IO_002151"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HIO9875327.1"
                     /translation="MNTAPKQYGFYMPAEWYPHERCWMAWPCHHETWSKIGLDKAKMA
                     YARVAKAIAQFEPVTLLVNPGDEDSAKNLCKGHNIEIISLPINDSWTRDTGATFLINN
                     ERQLAGVDWIHNAWGGNYADCSLDNFIASHLIKYTEAQYFHAPLVMEGGSFHVDGEGT
                     ILTSKECLLNSNRNPHLSQQEIEQYLINYLGAERIIWLNMGLIGDETDGHVDEIATFI
                     APGKVLCLITKDKEDPNYDRLQENFEILKSSKDARGRTFEVHTVEQPPATYLNGERLT
                     LSYINFYMANQGIVMPAFGYESFDRLAYQLFVQIFPGYQITQIDALDVFSGGGGIHCI
                     TQQQPKSHKLVE"
     gene            complement(5581..7998)
                     /gene="gyrB"
                     /locus_tag="AC06IO_002152"
     CDS             complement(5581..7998)
                     /gene="gyrB"
                     /locus_tag="AC06IO_002152"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIO9875328.1"
                     /translation="MSVDASYDSNNIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNSIDESLAGYCKEIFVTIHSDESITVKDDGRGIPVDIHKEEGKSAAEVIMTVLHA
                     GGKFDDNSYKVSGGLHGVGVSVVNALSEELHLLVRRHGKVYEQHYRHGVPESPIAETG
                     ESNSTGTQIWFKPSAETFSNIEFHYDILAKRLRELSYLNSGVCIHLFDERTQKKDTFH
                     YEGGIKAFVEHLNKNKTPVHPVVFSMTSEKDGIVIELAMQWNDGYQETIFCFTNNIPQ
                     RDGGTHMAGFRAALTRTLNNYIENEGYAKKAKVSPTGDDAREGLTAVLSVKVPDPKFS
                     SQTKDKLVSSEVKPAVESCVAEKFNDFLLENPTAAKSIVAKIIDAARAREAARRAREM
                     TRRKGALDIAGLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQARDRKFQAILPLKGK
                     ILNVEKARFDKMLSSQEVATLITALGCGIGPEEYDPDKVRYHRIIIMTDADVDGSHIR
                     TLLLTFFYRQTRELLERGYIYIAQPPLYKVKRGKQEQYVKDDEALFDYLTQCALDGAT
                     FYPAKEAPPISAKSLEELVQHFRRVEKIIKRYKRRYPSDILKRVAYMPMLHQEDFNNL
                     SKIESWCRQLHLSLQQVDSKTEQYKVDIQKLTEGNQYTPKITVYQHGLENDIPMQVEF
                     FLSKDYKEMVDLGHKLSNLIEEGAYIQRGDKSLPVENFEQVLNWLMDEAKKGQTIQRY
                     KGLGEMNPEQLWETTMDPEVRRMLQVSIEDAVAADEIFTTLMGDNVEPRRDFIETNAL
                     DAENIDI"
     gene            complement(8295..9356)
                     /gene="recF"
                     /locus_tag="AC06IO_002153"
     CDS             complement(8295..9356)
                     /gene="recF"
                     /locus_tag="AC06IO_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945765.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIO9875329.1"
                     /translation="MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALY
                     MLSCAHSFRSREVTPIISYGQNQLNVFAHAYDESTISVQKSITDGTQIKLNNQFCCTT
                     SQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
                     LLKSRATYEHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTNISCTIEYYK
                     GWDRKNAGQNIEELLQKSFDSDKNKLYTQYGPHQADLIINIEQYRVKHTLSRGQQKII
                     LIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIMHLTQQKGQFVITNLINNNNYN
                     ILPIHSGLFEVNCGAIKVL"
     gene            complement(9353..10456)
                     /gene="dnaN"
                     /locus_tag="AC06IO_002154"
     CDS             complement(9353..10456)
                     /gene="dnaN"
                     /locus_tag="AC06IO_002154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIO9875330.1"
                     /translation="MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLY
                     ITATDLEIEMSAQVACDSKNTSGSITVPAKKFIDIIRSLDENASPSIVFDKALVTIKQ
                     NRSTFKLATLPAENYPSSEDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLL
                     LEFDSNIISAVATDGHRMAISRYSCNNIAQTKLLIPKKGVQELLRLLNCINDEQVLLA
                     AGKSHIKLITNQFVFLSKLIEARFPPYTKAIPREQDKCIVIDCASLKRALSRIVILAH
                     EKSKAVLLHLQQGQLTLIANNNEQEEAIETLSAETQGDELKIGLNATYLLDVLTHFSE
                     GQIRLSMSNTESSILIESLVNDNYQYIIMPMKI"
     gene            complement(10470..11828)
                     /gene="dnaA"
                     /locus_tag="AC06IO_002155"
     CDS             complement(10470..11828)
                     /gene="dnaA"
                     /locus_tag="AC06IO_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIO9875331.1"
                     /translation="MSTTAWQKCLGLLQDEFSAQQFNTWLRPLQAYMDEQRLILLAPN
                     RFVVDWVRKHFFSRIEELIKQFSGDDIKAISIEVGSKPVEAVDTPAETIVTSSSTAPL
                     KSAPKKAVDYKSSHLNKKFVFDSFVEGNSNQLARAASMQVAERPGDAYNPLFIYGGVG
                     LGKTHLMHAIGNSILKNNPEAKVLYLHSERFVADMVKALQTNSINEFKRFYRSLNALL
                     IDDIQFFAGKDRSQEEFFHTFNALLEGQQQIILTSDRYPKEIEGMEERLKSRFGWGLT
                     VAVEPPELETRVAILISKAEQSNIELPYEVAFFIAKRIRSNVRELEGALRRVIANAHF
                     TGKPITIEFVHEALRDLLALQDKLVTIENIQKTVAEYYKVKVADLLSKRRSRSIARPR
                     QMAMALSKELTNHSLPEIGDHFGGKDHTTVIHACRKVKELIQDDSDFAEDYKNLMRIL
                     SS"
BASE COUNT         3662 a         2329 c         2191 g         3693 t
ORIGIN      
        1 gcaattgcat tgagactggc tgaaacaact cctgctcaag atcgttcacg agtatctcaa
       61 aatatcaaca atattttccc acaatctctg tttgccgcta aagtcggcca cgcttttgcc
      121 gttcgcagag acatagagcg actattgcta ggtgaaaggc ccgatcaatt cttctcaagt
      181 cgtgaattca agatcgattc ttgcatcgaa tttgcaagtt tattcaatgt aattaatgat
      241 aaagaatctt ccattgccgg aaaacttgcg ctgagaaccc ctgctgaaaa tcgcactgat
      301 gtagtaatga aaattaaagg tttttgtgcc gaggatagtg aacttgcgat aaaagtccaa
      361 tctgcttttg cattaagaag ggatatagag cgcgatctat taggtgataa tccagaacaa
      421 tttttcaata gccgagattt tagcgttgat ttatgccttg agtttgcaat tttattcccg
      481 gaattattaa aaggccatga aaaagctatc ggtgaaaaat tagctaagct ggatgctaaa
      541 gtacgctcag atatcagtag aaaactggaa atgatcaatg gagcagctca cgagcaatca
      601 agccctttca gaataatagc cagtgccatg gtgcctaaag aagagccttg cctgacaaag
      661 cccactcccg ttgtcttgcc atctactgaa gaaattatac caaagcaaga atcacccacc
      721 agtttacgca acaggaacgt tttatttaaa gaacaaacag aagaacacaa gaagaaaaac
      781 tctcagatgg aacccattgt ctcagattct gaagaagaag aagaagaaga gaaacagaat
      841 tgctggacta gtttttgtgg gttatttggc aagtaaagca gccatatctt ttgcccttac
      901 ttggttaagg gcaactttac acttttttac ccattcttta cacatcatgt agcaataatt
      961 catgttaata tttctcataa attcattgac ttattgcaat ctgctcgagt taaagacata
     1021 aattaacaat tgaacgccgc tttaaactta ataaatgatt cggacagaaa gactattgaa
     1081 caagtttact tttttagctt taaaaaaata gaagcccctt acctcacaaa aaatcaagga
     1141 aaaacgaagt gtgctgtcct aaatgaaagg aaagattcgc ttctaatcga tagttgcgag
     1201 ccttcaccac tttcagaata gctgccattc tgaaaattca ttagtaatta gtaacagcca
     1261 aaatgagcag tgaattgaaa ttaacgctag tacaaagaga gctttttttg ataacatagc
     1321 cagattttta atctgggttg atgatttaac ttaagcaaga gtaagccatt cggattcgag
     1381 ataaaaatta cttaacatca tttattggga actctaatga aagaaaacaa actaacggtc
     1441 gcactggttc aggaacaatg gtatgaaaat cctaaagagc atcaagataa actggcttct
     1501 ggaatatttt cagctaccca gcaaggagca aaactcgttt gtttacaaga gcttacctta
     1561 agcccttatt tctgtacacg tagtgacgtc gatccagccc cttatatgga ggacatcgcc
     1621 actggtccca ctgctcaatt tgttagccaa atggcaaagt cgaatcaaat tcatattacc
     1681 gcatcgcttt ttgaaaaagc tggttacaat accgcagttg cttttaatcc ccaaggagaa
     1741 ttgattgctg ttaccagaaa gcaacatatc cccagtggtg aaaaatatca tgaaaacttt
     1801 tacttcaagc cgggcgattc caattatcct gtccatacca ttgctggtca ccacttggga
     1861 ttgccaacat gctatgacca atggttcccg gagttatcaa ggatttatgg attaaaagga
     1921 gctgaaattt tggtctatcc cacagcgatt ggaggggaac cgactgcgcc aggatttgat
     1981 agccaaccca tgtggcaaaa agttatggta gcacaaggaa ttatgagcaa caccttcatt
     2041 attgcggtca accggattgg gtgtgaagat gatttaagtt tttacggaag cagctttatc
     2101 agtaccccta tgggcgaaat tttagttcag gcaccgcgta atgaacctgc cgtattggtt
     2161 gctgaactgg attttagcca acgtgaattg tggggacgat tattcccatt tccccaacaa
     2221 cgagagcctg aaacttatca tgaattagtc aaatcccgtt aatcctcttt ggaacgttag
     2281 aatgaacaat aatactctat cagatgacaa tctttacaac atagaaaatt ggggcgaggg
     2341 atacttccga attaactcca agggaaatat tgaaatcagt aaaaaacccg ggaaaccagg
     2401 tgttgaacta caaactatag ttgatgcagc tgatcgcgct ggcttgcatt tgcctttatt
     2461 gatccgttgt agtgatattc ttcatgatag agtccataga atatatcagg cgttcaccca
     2521 agctcgagaa gacacttatt atgccggtaa ttatcagctg gtttatccaa ttaaagtaaa
     2581 ccaggaacaa agtgtggtac gggaattgtt aaaatcgcct gataatcaga taggcctgga
     2641 ggcaggcagc aaacctgaat tgatggcggt tataggtatg ctgggtgacc atcagagtac
     2701 tatagtttgc aacggctata aagacacaag ttatattcgt acagcgctta ttgctcaaca
     2761 aatgggtcat aaggttttta ttgttattga aaaaaaatct gagttagaca ttattttaaa
     2821 ggagtctgca cgccttcaaa tcaaaccaac attaggtgtg cgaattcggc tggtcagcaa
     2881 gagtgctggc aagtgggaaa ataccggcgg ggttaaatca aagtttggat taaatgcgaa
     2941 acaggtactt gagctgattt tacaattaaa agccaatgat atgctggatt gtttgcagct
     3001 aatgcactgc catttaggtt cacaaattgc caatatccat gacatacgcc attgcatgca
     3061 ggaagtggcg agatactata ttgagctacg tcaactcaat gctccgatag gtaccatcga
     3121 tgtgggtgga ggtttgagtg tcgattatga ggggactcga tccaataaag gatgctcaat
     3181 gaactacagt cttgttgagt atgctacgca catcatgttg gctttaaaac acttatgtca
     3241 agaagctgag atatcagaac ctgatattat ctccgaatca ggacgagcat taacagccca
     3301 tcatgctgta ttaatttcta atattacgga tgtagagatc atcaataaag cgcccgttct
     3361 tcctgaaata tcggaagatg attcgcatgt cattcgagat atttacgata cttatcaagc
     3421 cattacagat agctcgccca ctgaaatata taattacgca gctcattcgc ttaatgaagc
     3481 gcattccctt ttcaagcatg gggtaattag cctacaggaa aaagccaagg tagaagcttt
     3541 ttatactaat atttgtatgg aaataaaaga taaactggat gaagaaaatc caagtgaaaa
     3601 ggctttgcta atgagaatca atgagtgcat ggctgccaag atattttgta atatctcttt
     3661 tttccaatct attcctgatg cttgggctat tggtcagatt ttcccggtag cccctatatc
     3721 tcaacttaat agaacactta gtatgcatag tgtcctgcaa gatttaacct gcgattcaga
     3781 tggtacaatt aagcaatacc ctggaagttc ctgcgttaca accacattga tgctcccttc
     3841 atacgatatc aataaccctt ataacatcgg atttttcctg gttggtgcct atcaggaaat
     3901 attgggtaat ctgcataacc tgtttgggga taccaattct ctagatgtaa agctttctga
     3961 tgatggacaa tttgagctgg atgatttagt cagcggcgac accgtaacaa acgtgttgaa
     4021 tttagcccat tttgatacca aaaagttaat tcaatcttat gaaaagcaat taattaaatc
     4081 tgaacttcct aaggaaaagg cactctctta cttaaacgaa ttaagaagta ttttttctca
     4141 attaacttac cttgatggaa atatagcaca gtgaatatga atacagcacc caaacaatat
     4201 ggtttttata tgcctgctga atggtatcct catgagcgat gctggatggc atggccctgt
     4261 caccatgaaa cctggtctaa aatagggcta gacaaggcta aaatggccta tgcacgtgtt
     4321 gcaaaagcta tagctcaatt tgagcccgtt actttattag taaacccagg agatgaagat
     4381 tccgcaaaga atttatgcaa aggccataat attgaaatta tctctttgcc aattaacgat
     4441 tcgtggaccc gcgatacggg cgctaccttt ttaattaata atgaaaggca gcttgccgga
     4501 gttgactgga tacataatgc ttggggtgga aattacgctg actgttcttt ggataatttt
     4561 atagcttctc atctaataaa atacactgaa gctcaatact ttcatgcgcc attagtcatg
     4621 gaaggtggtt cctttcatgt ggatggtgag gggactattt tgacaagcaa agagtgtttg
     4681 ttaaattcca atcgaaatcc acatttatct caacaggaaa tcgaacagta ccttattaat
     4741 tatctgggtg ctgaacgtat tatttggctg aacatgggat tgataggtga tgagacagat
     4801 ggtcatgttg acgaaatagc tacatttatt gctcctggaa aggtgttatg ccttattacc
     4861 aaggataaag aagatcctaa ctacgatcgc ttgcaggaaa attttgaaat tctcaaatca
     4921 tctaaggatg ccagaggacg aacatttgag gtccatactg ttgagcaacc acctgcaact
     4981 taccttaacg gagaaaggct cactctctcg tacatcaatt tttacatggc taatcaaggt
     5041 attgtgatgc ctgcttttgg atatgaatca tttgacagat tagcctatca gttattcgta
     5101 caaatttttc caggatatca aattacacaa attgatgcac tcgatgtatt ttctggtggt
     5161 ggtggtatcc attgtattac acaacaacaa cctaaatctc ataaattagt ggagtaatgg
     5221 cgaagacaat gccacttatt aattgagaaa tttcttacat attactgctc attatactcg
     5281 atgacatata ctgccaattg gttatactaa aagcatacag ggatgacatg gatatgtctg
     5341 acaggaagtc aactaaggga ccagtacaaa ggatgtactt cgctgcaagg acgcagcact
     5401 tattctccaa tttaccagta tacctcttta aatttttcta ttaagcaatc aaaaagcttt
     5461 ttttgaacac atcatcgtag taggaaaata agttaagtca ttggataaat cagctataag
     5521 ctagagaggc gtttgccact agcgaggcaa acgtcactcc agtttatcaa tactatctgt
     5581 ttatatatca atgttttcag cgtctaaagc attcgtttca ataaaatctc ttcttggttc
     5641 tacattgtcg cccattaagg tggtaaagat ttcatctgca gctacggcat cttcaatgct
     5701 cacttgcaac attcgtctaa cctcgggatc catggttgtt tcccataact gctcaggatt
     5761 catttcccca agacctttat agcgttgaat tgtttgtcct ttttttgctt catccattag
     5821 ccaattaagt acttgttcaa aattttccac aggcaaggat ttatctccac gctgaatata
     5881 ggcaccttcc tctattaaat tcgacaattt atgacctaaa tcaaccattt ctttgtaatc
     5941 tttagacaag aaaaattcaa cttgcattgg aatatcattt tccaagccat gctgatacac
     6001 tgtaattttt ggagtgtatt gattaccttc tgtaagtttt tggatatcta ctttgtattg
     6061 ttctgtttta ctgtctactt gttgtaagct taagtgtaat tgtctgcacc agctttcaat
     6121 ttttgacaga ttgttaaagt cttcttggtg tagcattggc atataagcca ctctttttag
     6181 tatatcgctg gggtatcttc tcttgtaccg ctttataatc ttctcaactc gtctgaaatg
     6241 ttgaaccaat tcttccagag atttggcaga tatgggcgga gcctctttgg cgggataaaa
     6301 tgtcgcccca tccaacgcgc actgagttag ataatcaaat aacgcttcat catctttaac
     6361 atattgctcc tgttttccac gctttacttt atacaaaggt ggctgtgcaa tataaatata
     6421 tcctcgctcc aatagctctc tggtttgtct atagaaaaag gtaagaagta aagttcttat
     6481 gtgggaccca tccacatcag catctgtcat aatgatgatt ctatgataac gtactttatc
     6541 tggatcatat tcttcaggac caatcccaca gcctaatgca gtaatcaaag tggctacttc
     6601 ttgagaagaa agcatcttgt caaaccgggc tttttcaaca ttaagaatct tgcctttaag
     6661 aggtaaaata gcctggaatt ttctgtctcg cgcttgtttt gctgagcctc ctgcagagtc
     6721 cccctcaact aaatacaact cggaaagcga tgggtctttt tcttggcaat cagctaattt
     6781 acctggtaaa ccggcgatat ccaaagcccc tttacgtcga gtcatttcac gagcacgccg
     6841 cgctgcctct ctagctcttg ctgcatcaat aatttttgct acgatggatt ttgctgcagt
     6901 gggattttct aaaagaaaat cgttaaattt ttcagcaaca caggattcta ctgctggttt
     6961 tacttcagat gaaaccaatt tatctttcgt ttgcgatgaa aacttgggat cagggacttt
     7021 tactgaaaga acagctgtta atccttcgcg agcatcatca cctgttggag atacttttgc
     7081 ctttttggca tacccttcgt tttctatgta attatttaaa gtgcgagtta atgctgctct
     7141 aaagccggcc atatgtgttc caccatccct ttgcgggatg ttgttagtaa agcaaaatat
     7201 ggtttcttga tagccatcat tccattgcat tgccagttca atgacaatac catctttttc
     7261 cgatgtcatt gagaaaacaa cggggtggac tggtgtttta tttttattta agtgctcaac
     7321 aaatgctttg atgccgcctt cgtagtgaaa cgtgtctttt ttctgtgttc tttcatcaaa
     7381 taagtgaata caaacacctg aattcaggta tgacaattca cgtaatcttt tagctagaat
     7441 gtcgtaatgg aactcaatat tagaaaaggt ttcagcgctt ggtttaaacc atatttgtgt
     7501 gccagtagag ttggattctc cagtttcagc aataggagat tcaggaacgc cgtgtctgta
     7561 gtgttgctca tacacctttc catgtctacg cactagtaaa tgcaactctt ctgaaagggc
     7621 attcaccaca gataccccta ctccatgcag accgcctgat accttgtaag agttatcatc
     7681 aaatttacct ccggcatgca ggactgtcat aatgacttca gcagctgatt ttccttcttc
     7741 cttatgaata tcaacaggaa taccacgacc atcatcctta acggtaattg attcatcgct
     7801 gtgaatggta acgaaaattt ccttgcaata acctgccagg gattcatcta ttgaattatc
     7861 tacaacctca aaaaccatat gatgcagacc ggttccatca tctgtatccc caatgtacat
     7921 gcccggtctt tttcttactg cgtctaaacc ttttaggact ttaatattat ttgaatcata
     7981 actggcgtct acgctcatca gctgccctct tgccgtatta tgctattaac aatgagtcta
     8041 ttataccatc ttttgtatct ggtttatatc ttaagtagag aactttacgc ttgtgtattc
     8101 tgaattattg acgttgattt ttttgaatat cattttgttt atttgatttt cataccatga
     8161 catttgagga gccatttgca gaatagcata tgtttacctt tggaatttta tattgcattt
     8221 gcattaaggt gttattgttt aataacgaga acttttcatt ccattaattt gtgttccacg
     8281 tgaaacattc tatattataa aactttaatt gcgccacagt ttacttcaaa caaaccagag
     8341 tgaattggca agatattgta attattgttg ttaattaaat ttgttataac aaactgaccc
     8401 ttttgctgtg tcagatgcat aattaaattt cgctggtgat agtcatctaa ctcagcagcc
     8461 aaatcatcaa taaggtataa gcaatcctta tctaatagct gaccttgagc cagtttaagt
     8521 gcgattagaa tgattttctg ttgtccacga gagagtgtat gctttactct gtattgctca
     8581 atgttaatta tcaaatcagc ttggtggggt ccatattgcg tatatagctt atttttatca
     8641 ctatcaaagg atttctgaag cagttcctct atgttctgcc ctgcattttt tctatcccac
     8701 cctttgtaat actcaattgt gcaggatata ttggttaaat cacttaagac ttggtagaat
     8761 ttaggttgcc attgcaaaaa atagtcattt ctagctttat ccagttgatt tgccaattga
     8821 cttagctgtt ggtcccaagg aataaaatgt tcataagtag ctctggattt taataatgca
     8881 tttcgttggc taagtatgcg cttgtaatcc ttccaaattt tcaaataatc atgtttcacg
     8941 tgaaacaatc cccaatcaag taagcttctc cttaccgagg ggccggcatc aataatttga
     9001 aaaatatctg agtaaataac ctggcaagga agcgcatagg ctagttgact tgtagtacag
     9061 caaaactggt tattcaattt aatttgtgtg ccatctgtta tagatttctg aacactaata
     9121 gtgctttcgt cataagcatg ggcaaaaaca ttaagttggt tttgaccata agaaataatt
     9181 ggagttacct cacgagaccg aaaagaatgg gcgcagctca acatgtaaag agcctctagt
     9241 aacgaggttt taccactgcc attaggtcct gtaatacaat taaaattggg gtttaggatt
     9301 aaactagtcg aagcgatatt ccgaaaatta tgaattctaa cttcagataa aatcatattt
     9361 tcataggcat aataatgtat tgataattat cattgactaa tgactcaatt aaaatactgc
     9421 tttccgtatt agacattgat aacctgattt gaccttcaga aaagtgagtt aaaacatcca
     9481 gcaaataggt tgcattaagt ccgattttga gttcatcacc ttgtgtttca gcactcaatg
     9541 tttcaattgc ctcttcttgc tcattattat tggcaattaa agttaactga ccttgttgta
     9601 aatgcaacaa gacagccttt gacttttcat gagctaaaat gacaatacga gataatgcac
     9661 gcttgagcga agcgcaatca ataacaatac atttatcctg ctctcttggt atagctttag
     9721 tgtaaggtgg aaagcgtgct tcgattaatt ttgacaaaaa tacaaattga ttggtaatca
     9781 acttaatatg agattttccc gctgccaaca aaacttgttc gtcattaata caattcaata
     9841 atcttaataa ttcttgcacc ccttttttgg gtataagtaa ttttgtttga gcaatattat
     9901 tacaggaata tcgcgatata gccattctgt gtccatcagt agcaaccgct gaaataatat
     9961 tactgtcaaa ctccagcaat aatccattta aaaatatacg aacatcctgc tgagacatag
    10021 caaaatgagt agattgcaat aactgcaaca aaaccaaacg cgggatagtc aactcgacat
    10081 cattgcattc atcttcacta ctgggataat tctcagcagg caaagttgcc agtttaaatg
    10141 tacttcggtt ttgttttatc gtcaccaaag ctttatcaaa aacgatagat ggcgaagcgt
    10201 tttcatctaa tgacctgata atatcaatga atttcttggc tggtactgta atagaaccac
    10261 tagtattctt ggaatcacat gccacttgtg cggacatttc aatttccaaa tctgtagccg
    10321 taatatataa aagtccatcc gacagtttta ataaaaaatg ggacaaaata gccaaagact
    10381 gcttcttatc tacggctccg gaaacagtaa gcaatggagt aagcagatct tcttttttaa
    10441 tggttagctc aaacaagatg gactccgtat caagaagata atattcgcat taaattttta
    10501 taatcttcag caaaatcgct atcatcctgg attaattctt taaccttccg acaagcatgt
    10561 atgactgtcg tatgatcctt accaccgaaa tgatcaccga tttcaggtaa actatgattg
    10621 gtcaactctt tactcaaagc cattgccatt tgccttggtc ttgcaataga ccggcttctg
    10681 cgttttgaaa gtaaatcagc gacctttact ttataatatt cagctaccgt tttttggata
    10741 ttttctatag taactagctt atcttgcaaa gcaagaagat cacgcaatgc ttcatgtaca
    10801 aattcaatag ttatcggctt accagtaaag tgagcattgg ctataactcg acgcaaggca
    10861 ccttcgagtt ctctgacatt cgaacgtata cgtttggcta taaaaaaggc tacttcataa
    10921 ggcaattcaa tattagattg ctcagcttta ctaattaata ttgccacgcg cgtttctaac
    10981 tctggaggct ccactgctac agtcaatccc caaccaaaac gagattttaa acgctcttcc
    11041 atcccttcta tttccttagg atatcgatca ctggtcaaaa tgatttgctg ttgcccttcc
    11101 agtaaagcgt taaaagtatg aaaaaattcc tcttgtgacc tgtccttccc cgcaaaaaac
    11161 tgaatatcat caataagtaa agcatttaaa gaacgataaa aacgtttaaa ttcattaata
    11221 gaatttgttt gcaatgcttt aaccatatct gcaacaaaac gttctgaatg caaatacaat
    11281 accttagcct caggattatt ctttaaaata ctattaccaa tggcatgcat gagatgtgtt
    11341 ttacctaagc ctaccccccc ataaataaat aagggattat aagcatcccc agggcgctca
    11401 gctacctgca tggacgcagc tctcgccagt tgatttgaat ttccttcgac aaagctgtca
    11461 aaaacaaatt ttttatttaa gtgactgctt ttatagtcaa ctgctttttt aggagcggat
    11521 tttagaggtg ctgtactaga tgaagtaaca attgtttcag caggcgtatc aacagcttca
    11581 acaggcttac tacctacttc aatagaaata gctttgatgt catcgccgga aaactgtttg
    11641 atcaactctt caatacgaga aaaaaaatgt tttcttaccc aatcaacaac gaaacgatta
    11701 ggcgctaata agattaaacg ctgctcgtcc atgtaagctt gtaaagggcg caaccaagta
    11761 ttaaattgct gagcagaaaa ttcatcttgc aacaaaccta aacatttttg ccaagcagta
    11821 gttgacacaa aataactcct aatttaaaca agggtggtaa aagaatactt aaaac
//
LOCUS       DBNCWK010000097        11711 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.097, whole genome shotgun sequence.
ACCESSION   DBNCWK010000097 DBNCWK010000000
VERSION     DBNCWK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.097"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..2298
                     /gene="legC1"
                     /locus_tag="AC06IO_002156"
     CDS             <1..2298
                     /gene="legC1"
                     /locus_tag="AC06IO_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC1"
                     /protein_id="HIO9875332.1"
                     /translation="DRVLEHKTNVVETLVRHLKDENFKCLKVDSNQLKAKYPCLIVTF
                     KYDEHRNKPENIPEGYSEFVTHMMIAQINLKLKEKNLSPLIDRRQSFGFLTPTLTDVH
                     TGVRLSLGLTPNKQWIECVEEGIKQTDKSLAKFTLKDKDDLLNLFRAGEFCGDGHKYL
                     YDLYKTGEKTQEEVKKFLQESKVKSECKDIYFSTKDITCIDKDKLKKGVLKRFYYFDS
                     KNGTITLYDNQHQHGSSLKVNADVIHYFLLKNFSSKDLIKLTEVQECWLKEAIDDAQR
                     NNPFQYIDRKYLSLYRKITRLYAQILSVEHNRGEEKDLAKLTRDLINLQNEVVTSLLK
                     TMPLITNDTSDDEHANKYAQKNFYSPAGMSALFAPLYAASKVFSYNNEMPISYGSDPY
                     AYFELLLSWNKTLDESQFVRSEVVKEKLENRAFKMLLENFANESNKTGLVKMGRSEVT
                     ICFKNIYTEIYSNQIERKQLDVDSIINSNLTAIKNALEINTENDRGAIQFNEEKLRSI
                     LKQGINIIQQSFPNYMEHVNSLENNPTVYFADNNPCVNKDHLEVRTAIEVLKDLCTKD
                     LCPKVFVLDTTSATEAQIEDFLNVFNQQDKIPILVTAASMVKHSEFGLDLWQGGINKA
                     YLSEKSEKNENSQNEFARFITELKNVTKGTEPGFTRFARRHVREAMNNINEAIKDSQD
                     NSEPQDVTCKDDVQNIPNNPISKESNRTEEGQTINRELDEYAPLLTKKGFFAVQIEKV
                     PEKNVNQNNKQKSLEEGEPPCCTII"
     gene            2728..4710
                     /locus_tag="AC06IO_002157"
     CDS             2728..4710
                     /locus_tag="AC06IO_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875333.1"
                     /translation="MNKTLLFVFGSIGLSLNTEISAAKGIVCKDYDGNPMTVKPKTIT
                     IFNNSEVTIYPVLATSKNAVNEWIQGCFRTTEPYPTNYVYKLYVNEGTGIAPGSSVKI
                     TLPLYSQLANDRYITWWNGGRIVLADKNERLRNENDQALSTPEDISCDGTNTECKLSV
                     YSSDVQFPENIYAQLSEYTFGDSIIPPKQSTRILKPENVGYNISYVDHVYMPVAIGPK
                     NNPYIGYSGSVMSLPTFRSHLNSFLETNLGHGWPVYNLNELKLPGGYNIFAQRSGTLP
                     PGDNVPVKPNDGFPPVLTVLACIQEDCTEEQKASLHFGESVQRMQNLWGSCVNWDEED
                     ISKYITQKVDCPQDLKEKLSAVQQFFKKNHQQYLQLYENGKCNLTPGIDPVPFNYWEA
                     IKHIYGWVPFNEGCGAGANPLAETNIPGWDHARIQSMYIHDLQYNYQNKPIPELLFNP
                     YVQLIHDKNYLSMDAYGFSVDDAVGFMSELGDGLIFTVGGTKGLENQQEFNYADGFSV
                     AIGVPLSLLDQVNTPMIKKYGVCVFNQDANDLNCQEVKQNVIMPTNSQIAGFRVGTVA
                     SYPIKVFFTDLNDNLYTFVVNTKFAPCSAEMDPSQCPTNKAEIIDTKSCIVTKSNGEK
                     HPKSNNWCQNANPNQQREKQLTKNFISFPQPVDYME"
     gene            complement(4843..6327)
                     /gene="lspE"
                     /locus_tag="AC06IO_002158"
                     /gene_synonym="gspE"
     CDS             complement(4843..6327)
                     /gene="lspE"
                     /locus_tag="AC06IO_002158"
                     /gene_synonym="gspE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE family T2SS ATPase variant LspE"
                     /protein_id="HIO9875334.1"
                     /translation="MENEEKSLKIPYSFAKGNGLVVGEIKENLAIVYHLPNTPLQAFA
                     EIKRLLQCELDLKQVDETTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSE
                     DLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
                     IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILD
                     KQAAQLDLNLLGMPEHTLKTVRQMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTR
                     NILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIA
                     VQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKT
                     PHELRQEEMELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIH
                     RNENIQTMENYLRPTTPSIREDGFKRVLCGDTSLAEILRVTSQN"
     gene            complement(6331..8706)
                     /gene="lspD"
                     /locus_tag="AC06IO_002159"
                     /gene_synonym="gspD"
     CDS             complement(6331..8706)
                     /gene="lspD"
                     /locus_tag="AC06IO_002159"
                     /gene_synonym="gspD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspD family T2SS secretin variant LspD"
                     /protein_id="HIO9875335.1"
                     /translation="MRSIVIILLAFVCCSLVRANDVNISTVTKDGNTFFYLQAGAYNL
                     EKDAKIRQNELAKLVDQKVEIKNLADKHLYLVQIGPIDDYQTARALKEKLSQKTERQF
                     NQINKNQTSSLVVSQLPKNTEQAQTAPPGSKLWNLRNADIRAVIAEVSRITGKNFVID
                     PRVQGKVSIVSSTPLSSRELYQVFLSVLQVSGYAAIPNGEIIKIIPNIDAKTQSPDLL
                     SGMKSPPRGDDMMVAVIPVHYVPSEQLVPVLRPLMPQWSSVSAYAPSNMLILSGRANN
                     IKSLAEIIKQVDSSSANGIDMVRLHHALAMDVANTLKDLVKTQPGIGSRTQITIAADD
                     RSNSILVSGSKTDRIRLRMLILKLDKDSSTGVNSNTQVVYLNYLRAEDLVPILAGIAQ
                     ANFSGNVGTTIGTITRPALDSTNPASSLANTSADGQSSSLSSTSTSSAPMNASGATAN
                     TTSASTQNEGSTKPTVQIIGEPNTNSIILNAPASIIRTLKTVISQLDIKPAQLLIEAL
                     VAEVDEKDVNNLGIEWGSNQQTGNPKDFRPGFAIINSKTRIDDFQAQIYALAREQRAN
                     ILSTPSVVVLDNRQAKILIGKQVSIATTSYPNNAGGTTTASPFTTFDRVNVALHLYVR
                     PQITRGQGIQLQIDQGNDTLDPATATTDNTTTPTFNISSIVTSVHVESGDVVVLGGLI
                     QDSIGNDNNKLPILGDIPGIGRLFQRNIRNRDKRVLMVFIKPIILRNERDNLHVTGEK
                     YNYVRQYQLDWIRSQEAFEQTDDETVLPPLTQGNLPVPFSTPPQYSSKMTK"
     gene            8879..9907
                     /locus_tag="AC06IO_002160"
     CDS             8879..9907
                     /locus_tag="AC06IO_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIO9875336.1"
                     /translation="MNNATIKWGILGTSFISEVMANAIQESKTSELVAIGSRTSEIAK
                     RFSEKFAIPKFYDNYQSLILDSDIDVVYIGLPNHLHKEWIIRCARAGKNILCEKPFVI
                     GIEEIHEVISVIERTNVFCMEALMYRYHPFIKKLQEIIQSNTIGKIKLYKATYTANIA
                     EIANPVAGGSIRNLGCYPISLVRLLANAEPIEICGIGRMNSRNNTDSQASVLLKFEDD
                     SIAAVSTADDIEMHWQFEVYGTKGHLKVETNPWLPGCEGNKIYISLNTESTPREISAK
                     AEKSLYTYQIDFINEQVLKGDSRQFSKTSLLDSMGNTIVLETWLKQILPSKPGLELSR
                     LKDRLPHA"
     gene            complement(9928..>11711)
                     /locus_tag="AC06IO_002161"
     CDS             complement(9928..>11711)
                     /locus_tag="AC06IO_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIO9875337.1"
                     /translation="YGSTTGDFIGVDREQKGIIGLTHVINSVSPPVNVRYQLNEQGEI
                     IGKKLLMRHYDPRVRPWYQQAIKAGKPIWTNVYTFYVFDKSDFLIPGITGASPIYNKN
                     KQIKGVIALDLTIDGLQHFIRELELTKNTMIYVINNESNIIAFRTPQNKKDIRGKKLT
                     PEWIKKLNIPLKKLDFNGFKPIIKSYTHNNQKYFLAYQPISSTNEKALWHIIIIVPET
                     DVTEPLKDLSMRYILLTILVLLIGTLLVRIVSQRISNPIIQLTEEAKEITMLNLKPRP
                     LLKTRIKEVSYMDKTLSSLRSSLASFQRYVPGSLVKKLMRSGRIAQVGGQSQTITILF
                     SDIKDFTSISESTSPQKLMTYLSDYFQSMTEIVIQHEGTLDKYIGDAIMALWNAPSKD
                     TNHALHACLAAKIMIERLSLLNQKNKHLGFPEFKIRIGIHTGDAVVGNVGSEDRLSYT
                     ALGDSVNLASRLESINKNYHTQIIVSHATFTQVSEKFPFRLLDEVAVRGKRESIEIYE
                     LITAQNLENLEQHKKEFQKAFSLYQKGSWKESISAFAKLTPAYSGDPLASIYIERCKV
                     LAVNPPVNWDGIWREGKTDYSLLGKFD"
BASE COUNT         3693 a         2010 c         2290 g         3718 t
ORIGIN      
        1 gatagggtat tggaacataa aacaaatgtg gtagaaaccc tggttcggca tttaaaagac
       61 gaaaatttta agtgtctaaa ggttgatagc aaccaactta aagccaaata tccttgttta
      121 attgttacct ttaaatatga tgagcatagg aataaacccg aaaatatccc tgaaggatac
      181 tctgagtttg ttacccatat gatgattgct cagattaatt taaaattaaa agaaaaaaat
      241 ctgtctccat tgatagatag gcgacaaagt tttgggtttc tcactcctac attaactgat
      301 gttcatacgg gtgttagatt atcacttggc cttacaccaa ataaacagtg gatagaatgt
      361 gttgaagaag gaataaaaca gacagataag tccttagcaa aatttacgtt gaaagacaaa
      421 gatgatttac tcaatctatt tagagcggga gagttttgtg gggatggaca taaatacctt
      481 tatgacttat ataaaacagg agaaaaaaca caagaagaag taaagaagtt tctacaagaa
      541 tcaaaagtga aatcagaatg taaggacatt tatttttcaa caaaagacat aacgtgtatt
      601 gataaagata aattaaaaaa aggcgtattg aaaagatttt attactttga tagtaaaaat
      661 ggaacaatta ctctttatga taaccagcac caacatggat cttctttaaa agttaatgca
      721 gatgttattc attattttct tttaaaaaat ttttcctcta aagatttgat aaaattgact
      781 gaagtacagg agtgttggtt aaaagaagca attgatgatg cgcagaggaa taatccgttt
      841 caatatattg accgaaaata tctatctcta tatagaaaga ttactcgtct ttacgcgcaa
      901 atacttagtg ttgaacacaa tcgtggagaa gaaaaggatc tagctaagtt aacgagggac
      961 ttgattaatc ttcaaaatga ggttgtcact tcattattga aaacaatgcc cttgattacg
     1021 aatgatacca gtgatgatga gcatgccaat aaatatgcac aaaaaaattt ttactcacca
     1081 gcaggtatga gtgctctatt tgcgccgctt tatgcagcat caaaagtgtt tagttataat
     1141 aatgaaatgc caatttctta tggcagtgat ccttatgcat actttgagtt attattatct
     1201 tggaataaaa ccttagacga atcacaattt gtgcgttctg aagttgttaa agaaaaactt
     1261 gaaaatcgcg cttttaagat gttattagag aattttgcaa atgagtcaaa taaaactggc
     1321 ttggtcaaaa tgggaagatc tgaagtaacg atctgtttta aaaatattta tactgaaatt
     1381 tatagcaatc aaattgaaag aaaacaactt gatgtagatt caattattaa ttcaaattta
     1441 acagcaatta aaaacgcttt ggaaataaat actgaaaacg ataggggggc aatccaattt
     1501 aatgaggaaa agcttaggtc aatattaaag caaggaataa atattatcca acaatctttt
     1561 ccaaattata tggagcatgt aaatagcctg gaaaacaatc ctacagtgta ttttgctgat
     1621 aacaatcctt gtgttaacaa agatcatctg gaggtaagaa ctgccataga agttttaaaa
     1681 gatctttgca ctaaagatct ttgcccaaaa gtgttcgtcc tggacacaac ttctgcaact
     1741 gaagcccaaa tagaagattt tttaaatgtg tttaatcaac aagataaaat tccaattctt
     1801 gtgactgctg caagtatggt taaacacagt gaatttggat tagatttatg gcagggagga
     1861 atcaacaaag cctatttgtc agaaaaatcg gagaaaaatg aaaattcgca aaatgagttt
     1921 gcacgcttta taacggaact caaaaatgtt accaaaggaa ccgaaccagg ctttacacga
     1981 ttcgcaagac gacatgttcg agaagcaatg aataatataa atgaggcgat aaaagattca
     2041 caagataata gtgaacctca agatgtaaca tgtaaagatg atgttcaaaa tataccaaat
     2101 aaccctattt ctaaagaaag caataggaca gaggaaggac agacgattaa tagggagctt
     2161 gacgaatatg cgcccctact tacaaagaaa ggattctttg cggtacaaat tgaaaaggtg
     2221 ccagaaaaaa atgttaatca aaataataaa caaaaatctc ttgaggaagg agagccgcct
     2281 tgctgcacaa taatttaact tccaagattt tgtttttatt tatagtcgtt caaatttgat
     2341 ctgagggttg caggtttttc aagaagcact tctcagatca aattacacaa tccttttcgg
     2401 tataaaatgc taattatatc tgcaatatgt aactgatttc ttcataatag acactgacac
     2461 cccccattac agcgaagtgc atcgtagttg aggcatgctt tgattgtaga tgatatttta
     2521 tattaagctg tatcaacacg gttcattatt aggaaataaa gctcttcaaa ttaactccgc
     2581 taaattgaga atgattagta tgtaagaata taaattctag ctggtatata gggtaatacc
     2641 ataatttgct cacctgacag atgattgtta atttaaaaat tactttttgt gctattttta
     2701 taaaatatta tacaaagaga aattaatatg aacaaaacac tgctttttgt cttcggaagt
     2761 ataggtttgt cattgaatac agagatttct gctgctaaag gcattgtgtg caaagactac
     2821 gatggcaatc caatgacagt caaaccaaag acaatcacta tatttaacaa ctctgaagtt
     2881 acgatttatc ctgttttggc aaccagcaaa aatgcagtaa atgaatggat tcaaggctgt
     2941 tttcgtacca cagaacctta tccaacaaac tatgtttata aattgtatgt gaacgagggg
     3001 acaggaatag ctccaggttc ctcagtaaaa attaccttgc ccttatatag tcaattagca
     3061 aatgatcgtt acatcacttg gtggaatgga gggcgtatcg ttttagctga taaaaacgaa
     3121 agattgcgaa atgaaaacga tcaggcatta tcgacccctg aagacattag ctgtgatggg
     3181 acaaataccg agtgtaaatt gagcgtatat tctagcgacg ttcagtttcc agaaaatata
     3241 tacgcgcagt tatcagaata tacattcggc gactctatta ttccacccaa gcagtcaaca
     3301 agaatattaa agccagaaaa tgtaggctat aacatttcct acgtagacca tgtttatatg
     3361 cctgttgcaa tagggcctaa aaataatcct tatattggat atagcggttc agtaatgtcc
     3421 ttgcctacat ttagaagcca tcttaattcg tttctagaga cgaatttagg acatggttgg
     3481 cctgtttata atcttaatga attaaaatta ccaggaggtt ataatatttt tgcacaaaga
     3541 tctggcacat tacctccagg ggacaatgta cctgtcaagc caaatgatgg ttttccacca
     3601 gtattaacag tgttagcttg cattcaggag gattgtaccg aggaacaaaa ggcatcactc
     3661 cattttggtg agtcagttca acgtatgcag aatttatggg gttcttgtgt caattgggat
     3721 gaagaagata tcagcaaata cataacccaa aaagtggact gcccacaaga cttaaaagaa
     3781 aagcttagtg ctgtacagca attttttaaa aaaaaccatc aacaatattt gcagctgtat
     3841 gaaaatggga agtgcaattt aacccccggc atagatcctg tgccatttaa ttactgggaa
     3901 gcgattaagc atatatatgg ttgggttcct tttaacgaag ggtgtggtgc tggcgctaat
     3961 ccattggcag agacaaatat cccaggatgg gatcatgcta ggattcaatc catgtacatt
     4021 catgatttac agtataacta ccaaaataag ccaatacctg agttattatt caatccttat
     4081 gtccaattaa tccatgataa aaattatctt tccatggatg cttatgggtt ttctgtcgat
     4141 gatgctgttg gctttatgag tgagctagga gatggattga tttttactgt tggtgggact
     4201 aaaggtcttg aaaatcaaca agagtttaat tatgccgatg gtttttctgt ggccattggt
     4261 gtgccattat ctttattaga ccaagtaaat actcctatga ttaaaaagta tggtgtatgt
     4321 gtgttcaatc aagatgccaa tgacttgaat tgccaggaag taaagcaaaa cgttattatg
     4381 cctactaata gtcaaatagc gggatttaga gtaggtacgg tagcttcata tcctatcaag
     4441 gtatttttca ctgatttgaa tgataacctg tatacctttg tcgtcaatac gaagtttgcc
     4501 ccatgctctg cagaaatgga tccatctcaa tgtccaacga ataaggcaga aattatcgat
     4561 acaaaatctt gtattgttac caagagcaat ggagaaaagc atcccaagtc gaataattgg
     4621 tgtcaaaatg ctaatccaaa ccaacaaaga gaaaagcaat tgactaagaa tttcatcagt
     4681 ttcccacagc cggtagatta tatggagtag gtgctgaagt ttaatgcgat tttagaactt
     4741 cttcttaggc taattacttt gtttaagcaa gctgataaat taaatgatca atatcttgaa
     4801 gacgtggtgt gactgtttaa aaattgctaa agtatgggta atttagtttt gagaggttac
     4861 tcttaaaatt tctgcaagtg acgtatcgcc acataatact cttttaaaac catcttcacg
     4921 tatgctcggt gtggttggtc gtaaataatt ttccattgtt tgaatatttt cattacgatg
     4981 aatcattcct ctcagggttt catctacgat aattaattca taaattcctg ttctcccctt
     5041 ataacccaag tggttgcaat agtcgcatcc cttgggttca aagacctgag atacatcagc
     5101 atcaggttta agacccatta actccatttc ttcctgtctt aattcatgag gtgttttgca
     5161 atgagagcat aatttcctta ccaaacgttg cgcaatgagc ccgactatac tggatgacaa
     5221 taaaaaagac tcaactccca tatcatgcag gcgagttaat gctcccaatg cagtattggt
     5281 atgaagggtt gatagtacca aatgccctgt taagctggct tgaacagcaa tttcagccgt
     5341 ttccaaatca cggatctctc ctatcatgac aacatccgga tcttgacgta atatggccct
     5401 taatcctttg gcaaaagtca tttgaacttt agtattaacc tgcgtttgtc ctacgccagg
     5461 caggtcatat tcaatgggat cttcaatggt taaaatattg cgagtcacct gatttagttc
     5521 ggtgagcata gcatagagag aggttgtctt gcctgaacct gtggggccag tgactaaaat
     5581 aatgccatga ggttcagcaa tcatttgtcg tactgttttt aatgtatgtt caggcatacc
     5641 caaaagattt aaatccagtt gagcagcttg tttatccaat atccttaaaa ccactcgttc
     5701 tccatggttt gagggtaaag ttgaaaccct gacatcgata ttgtgtcccc ctatacgcaa
     5761 tgaaattcga ccatcttgag gaattctctt ttcggcgata tccaatttgg ccatcacctt
     5821 aactctggaa atgaccaaag gagcgatagc tcgttgaatt tcaaggacct cttgcagaac
     5881 accatcaatt cgattgcgca ccaagactct gtcttcatat gtttcgatgt gtatatcaga
     5941 tgctttttgc ttaatggctt gagtgaacag agcgttcagt aagcggataa ttggggcatc
     6001 gtcttggttt tccaataagt cttcactgac tggaagttga ctggccaaca tggataaatc
     6061 catatcctct tccatgcctt ctgcggcatc cagtattgaa gatttagatt ggtaaatatt
     6121 ggctaaatgt tgttgaaaag tggtttcatc aacttgtttt agatcaagtt cgcattgcaa
     6181 taatcgcttt atctcggcga acgcttgcag aggtgtgtta ggtaggtgat atacaatagc
     6241 aagattttct tttatttctc ccacaacaag tccattgcct ttggcaaaac tgtaaggaat
     6301 tttaagtgac ttttcttcat tttccatgat ttatttggtc atttttgatg agtattgggg
     6361 aggagtacta aaaggtactg gtagattacc ttgagttaat gggggcaata ctgtttcatc
     6421 atctgtttgt tcaaaagctt cttgagatcg aatccaatcc agctgatatt ggcggacata
     6481 attgtatttt tcgccagtga catgcaagtt atctctttcg ttgcgtaata tgataggttt
     6541 aataaatacc atgagtactc gcttgtctct gttgcgaatg ttacgttgga acaatctgcc
     6601 tattccaggg atatctccta aaatgggtaa tttattatta tcatttccaa tactgtcctg
     6661 aattaatcct ccaagtacta caacatcacc actttctaca tgaactgaag ttacaatact
     6721 gctgatatta aaagtggggg ttgttgtatt gtctgttgta gcagtggcgg gatctaatgt
     6781 atcattccct tgatcaatct gtagttgaat tccttgtccg cgagtaattt gcggccttac
     6841 ataaaggtgc aaagcgacgt taaccctgtc aaatgttgtg aaaggacttg ctgtggtagt
     6901 gcctcctgca ttattggggt agctggttgt tgctattgag acctgtttac ctatgagtat
     6961 ttttgcttga cgattatcaa ggactactac ggagggagtt gataaaatat ttgctctttg
     7021 ctctcttgcc aaggcataaa tttgagcctg gaaatcgtca atcctggttt tactgtttat
     7081 aatggcaaag cctggtctga aatccttggg attgcctgtt tgttgatttg aaccccactc
     7141 tattcccaga ttgttaacgt ccttttcatc cacttcagca acgagggcct caattaatag
     7201 ttgagctggt tttatatcta gctgagaaat gactgttttt agagtgcgaa taattgaagc
     7261 aggtgcatta agaataatag agttagtgtt cggttctcct attatctgta cagtaggctt
     7321 ggtagagcct tcattttgag tacttgctga tgttgtattt gctgttgctc ctgacgcatt
     7381 cataggggcg gaactggtcg aagttgagct taatgaagag ctttgtccat cggcagatgt
     7441 attggcaaga ctggaagcag ggttggtgct gtctaatgcg ggtcttgtaa tggttccaat
     7501 ggttgttcct acatttccac tgaaattggc ttgggctata cctgccagta tggggactaa
     7561 atcttcggct cttaagtaat tcaggtaaac aacttgtgta tttgaattga ctcctgttga
     7621 actgtcttta tccagtttca atataagcat tcgcaaacga attctatcag tcttgctccc
     7681 actgactaaa attgagttgg aacggtcatc tgcagcaatg gtaatttgtg ttctgctgcc
     7741 gataccaggt tgagttttaa ccaggtcttt taaagtgttt gcaacatcca tagccaaagc
     7801 atgatgtaaa cgaaccatgt ctatgccgtt tgcacttgag ctatctactt gtttgataat
     7861 ttccgcaagg cttttaatat tgtttgctcg accagacaag atcaacatat tagaaggagc
     7921 ataggcagat acgctactcc attggggcat tagtggcctt agaacaggta ccagctgttc
     7981 agaaggaaca taatgaacgg gtattacggc gaccatcata tcgtcacctc gtggaggact
     8041 cttcattcca ctgagtaaat caggtgactg tgtttttgca tcaatattgg gaataatttt
     8101 gataatttct ccgttaggta ttgcggcata acctgatacc tgcagtactg agagaaagac
     8161 ttgatatagc tcgcggctgg ataacggcgt tgatgaaaca atcgatactt ttccttgtac
     8221 tctgggatca ataacaaaat ttttacctgt aattcgtgag acttctgcga ttacggctct
     8281 aatatcagcg ttacgtaaat tccataattt actgcctgga ggagccgttt gcgcttgttc
     8341 cgtattttta ggcaattggc ttaccacaag tgaagaggtt tggtttttgt taatttgatt
     8401 aaactgcctt tctgtttttt gagataattt ttctttgagt gctcttgcag tttggtaatc
     8461 atcaattgga ccaatctgca caaggtaaag atgtttgtca gccaggtttt taatttcaac
     8521 tttttgatcg actaatttgg caagttcatt ttgacgtatt ttagcgtctt tttcgagatt
     8581 ataggcgcca gcttgaagat aaaaaaatgt attgccatct ttggttacgg ttgaaatatt
     8641 gacatcatta gcacgtacta atgaacaaca aacgaaggct aaaagtataa ttactatcga
     8701 gcgcatttaa aaaccgtaaa ataatggata aagacataat aactaaattt gactgcttac
     8761 catagctttt ttaataactt agagcaggaa atttaagtgg tttttactgc tatggtttta
     8821 ctcgtgtcat ttatgcgttt taatatacag tcagcaattt tttagccgga aggaatatat
     8881 gaataatgcg acaataaaat ggggaatttt aggaacaagt tttatctctg aagtgatggc
     8941 taatgcaatt caggaatcaa aaaccagcga gcttgttgct attgggagtc gaacaagcga
     9001 gatagccaaa cgattttctg aaaaattcgc tattccaaaa ttttatgaca attatcaatc
     9061 attaattctc gatagtgata tagatgtggt ttatataggg ttgcctaatc atctgcataa
     9121 agaatggatt attcgttgtg cgcgggcagg aaaaaatatt ttgtgtgaaa aaccttttgt
     9181 tattggtata gaagaaatcc atgaggttat ttcagtaatt gagagaacaa atgttttttg
     9241 tatggaagct ctaatgtacc gatatcatcc ctttataaaa aaattacaag agatcattca
     9301 aagcaacaca ataggtaaga taaagttgta taaagcgaca tacacagcaa acattgcaga
     9361 aatagccaat cctgttgcgg gaggaagtat acgtaatctc ggatgttacc caatttcctt
     9421 agtgcggctg cttgctaacg cagagccaat cgaaatctgt ggtattggaa gaatgaactc
     9481 caggaacaat actgatagtc aagccagcgt tcttttaaaa tttgaagacg attcaattgc
     9541 agcagtttcg actgccgatg atattgaaat gcattggcaa tttgaggttt atggaacaaa
     9601 aggccattta aaagtagaaa cgaatccttg gttgcctggc tgtgagggta acaaaattta
     9661 tatctctctc aatactgagt caacacccag agaaataagc gcgaaagcag aaaagtcact
     9721 ttatacctat caaatagatt ttatcaatga gcaagtattg aaaggggatt ccagacaatt
     9781 tagcaagacg tctttattag atagcatggg aaatacaatc gttctggaaa cctggctaaa
     9841 acaaatactc ccttcaaaac caggccttga attatccaga ttaaaagaca ggctgcctca
     9901 tgcataagca aaatggttct ggtgatacta gtcaaatttt ccgagaagag agtaatctgt
     9961 ttttccttcg cgccagatac catcccaatt gacaggtgga ttaactgcta ataccttgca
    10021 gcgttcgatg taaatggaag ctaatggatc accagagtag gccggagtaa gttttgcaaa
    10081 agcgcttata ctttctttcc aggaaccttt ttgatagaga gaaaatgctt tttgaaattc
    10141 ctttttatgc tgctcaagat tttcaagatt ttgggctgta atcagttcat atatctcaat
    10201 actttcacgc ttacccctta cagctacttc atctaacaaa cggaaaggaa atttttcaga
    10261 tacttgagtg aaagtggcgt gactgacaat tatttgagta tgataatttt tattaattga
    10321 ttccaggcga cttgccagat tgactgagtc gcccaaggcc gtataactta gcctgtcctc
    10381 tgaaccaaca ttacccacaa cagcatctcc ggtgtgaatg ccaattctga ttttaaattc
    10441 gggaaatcca agatgtttat tcttttgatt taacaggcta aggcgctcaa tcatgatttt
    10501 ggcagccagg catgcatgca atgcatgatt ggtatctttt gaaggggcat tccataatgc
    10561 catgatagca tcgccaatgt atttatccaa agtcccttca tgttgtatta ctatctcggt
    10621 catggactgg aaataatcgg ataaataggt cattaatttt tgtggtgagg tggattctga
    10681 aatacttgta aaatctttta tatctgaaaa taaaatagta atcgtttggc tttgccctcc
    10741 aacttgagca atccttccgc tacgcattaa tttttttacc aaagatccgg gaacatagcg
    10801 ttgaaatgaa gctaagcttg atcgcagaga tgatagtgtt ttatccatat aactgacttc
    10861 tttaatcctg gtcttcagaa gtggcctggg tttgaggtta agcatggtaa tttctttagc
    10921 ttcttcagtc agttgaataa taggatttga tattctttgt gaaacgatac gaactaacaa
    10981 tgttccaatc agcaagacta atatagttaa caaaatatag cgcatgctta aatctttcag
    11041 gggttctgtc acatccgttt caggaacaat aataataata tgccacagtg ctttctcatt
    11101 cgtactggat ataggttggt atgctaaaaa atatttttga ttattatgag tataagattt
    11161 gataatgggt ttaaaaccat taaaatccag ttttttaagt ggaatattca gcttttttat
    11221 ccattccggg gttaattttt ttccacggat gtctttttta ttttgagggg ttctgaaagc
    11281 aatgatattg gactcattat taatcacata aatcatagtg tttttagtta attccagttc
    11341 tcggataaaa tgttgtaaac catcaatagt caggtcaaga gcaattactc cttttatttg
    11401 cttattttta ttatatattg gtgaagcccc ggtaattcca gggataagga agtcactttt
    11461 atcaaataca taaaatgtat atacgtttgt ccatatcggt ttacctgctt ttatagcttg
    11521 ttgataccac gggcgaactc tcggatcata atggcgcatt agcaattttt ttccgattat
    11581 ttctccttgt tcgtttaatt gataacgaac gttgactgga ggtgatacgg aattaatcac
    11641 atgggttaat cctattattc ccttttgctc tctatctact cctataaaat caccagttgt
    11701 tgaaccatag t
//
LOCUS       DBNCWK010000098        11708 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.098, whole genome shotgun sequence.
ACCESSION   DBNCWK010000098 DBNCWK010000000
VERSION     DBNCWK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11708
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.098"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(269..1693)
                     /gene="lpdA"
                     /locus_tag="AC06IO_002162"
     CDS             complement(269..1693)
                     /gene="lpdA"
                     /locus_tag="AC06IO_002162"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIO9875338.1"
                     /translation="MANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGV
                     CLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLK
                     ALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKR
                     IFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLV
                     NILQKRMTKKGVKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAVGRKPN
                     GGAINAEKAGIRVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEV
                     IAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGR
                     EEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSE
                     TIAQSAEAFEGTITDLYLPKKKKK"
     gene            complement(1782..3416)
                     /gene="aceF"
                     /locus_tag="AC06IO_002163"
     CDS             complement(1782..3416)
                     /gene="aceF"
                     /locus_tag="AC06IO_002163"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HIO9875339.1"
                     /translation="MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEK
                     ASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSDTTTNISSSQEQKAEPEKQNLQT
                     RAEEQSVDTKATASIPGSKEIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
                     DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTPGKSETPEIEISQIKNISEQS
                     IKEIEKPYEELKSEPISINSLEIAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKS
                     RITKEDLQNYIKVRLSEKTTPGGFSLPSNPAIDFSKFGSIETKPLNKIKKLTGTNVHR
                     SWITIPHVTQFDEADITDLEAFRKSESESAKNQDYKLTLLAFVCSVVCKALHAYPQFN
                     ASLDASGENLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREK
                     GLTPIDMSGGCFTISSLGGIGGTAFTPIVNSPEVAILGLSRSIIKPIYDNKEFKPRLM
                     LPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL"
     gene            complement(3474..6137)
                     /gene="aceE"
                     /locus_tag="AC06IO_002164"
     CDS             complement(3474..6137)
                     /gene="aceE"
                     /locus_tag="AC06IO_002164"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIO9875340.1"
                     /translation="MTNETNLDLDPIETREWLDALQAVLFNDGPERTAFLLQQLLNKA
                     NAEGVKLTSSINTPYRNTIKPHEEKQMPPDEGIGKRINALIRWNAVAMVLRAGKYAPE
                     LGGHIASYASASTLYETGFNYFFKGQKGENGGDLLYIQGHSAPGIYARAFLEGRLTEK
                     QLEKFRQEVEVDGLSSYPHPWLMNDFWQFPTVSMGLGPLQAIYQARFLKYLENRGLIK
                     AEGRKVWAFLGDGEMDEPESVGALSIAAREKLDNLIFVVNCNLQRLDGPVRGNGKIIQ
                     ELEGLFRGAGWNVIKVIWGGRWDPLFARDNQGWLQKRMEECLDGDYQSYKANDGSYVR
                     QHFFNQYPELKKMVENMSDEEIWRLNRGGHDPQKVYAAYARAVEHKGTPTVILAKTIK
                     GYGMGAAGEGQNITHQQKKMTIDQLKAFRDRFNIPISDDKIADIPFYKPDDDSPEIKY
                     IKKQREALGGYLPHRSTEVEQLKIPDLGEFSSITKGLGDREISTTMAFVRILSVLLKN
                     KDINSRIVPIVPDECRTFGMEGLFRQIGIYSPVGQLYTPVDHEQVMYYREAVDGQILE
                     EGINEAGAFCSWIAAATSYSSNKLAMIPFYIYYSMFGFQRIGDLAWAAGDMQARGFLL
                     GGTAGRTTLAGEGLQHQDGHSHVLASTIPNCISYDPTYAYELAVIIQNGLHRMYEKQE
                     NVFYYITIMNENYSHPDMPAGVEEGIIKGMYLLKENKKKSKNHVQLMGCGTILREVIK
                     AAEMLEEDFSITSDIWSVTSFNELRKEGLAVERYNNMHPQNKPQESYVTSQLKGRRGP
                     VIASTDYMRIYADQIRPFVPNRYITLGTDGYGRSDTRTQLRHFFEVDAKFIVLAALNA
                     LVAEGSLDKAKIVDAMKRYNINQDKLDPMTH"
     gene            complement(6165..6554)
                     /locus_tag="AC06IO_002165"
     CDS             complement(6165..6554)
                     /locus_tag="AC06IO_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIO9875341.1"
                     /translation="MNTAFRLLFCLIILELSACATLKSKITHHKTLSQCQQTCFQQLN
                     YCKQNCTDNCRDCSTKVDHFAKENYLEYLHEVKIQGGYITRGLQSYRDPLQCRKVTCN
                     CSADFNACNQGCSGVIQKKLQPVPYCS"
     gene            complement(6593..7345)
                     /locus_tag="AC06IO_002166"
     CDS             complement(6593..7345)
                     /locus_tag="AC06IO_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875342.1"
                     /translation="MKLLVIVLCLLSERFLIHSVSYQRFSWFNNYCQFLKKFIDKNEY
                     FSNPWATLIAIILPIVFLTFLVYFSLQSILFGLFGLILSLFIFYYCLGPQNAFYPILK
                     KQTNQTETDAIGEYFAEVNSQLFAVVFWYIIAGPIAALTYRLIALCKEINFISTQASQ
                     ITSILEWIPARITALLFLLVGNFQRGFHLFVQYVLTSPDSNDKILRGCGLQAVRINDT
                     EEVPMAAAENLVEHATIVLLVFIALFTLVAWL"
     gene            complement(7350..8522)
                     /locus_tag="AC06IO_002167"
     CDS             complement(7350..8522)
                     /locus_tag="AC06IO_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIO9875343.1"
                     /translation="MHDGSVFYTIFLIFAGAAFFSTLVLYTKQSLLVAYITLGAILGP
                     WGFKLVSDVTVVKQIGDIGIVFLLFLLGLHLQPQNLIHMLRKVTWIAIVSSILFAALA
                     YGIGRWFGLTVTESLILGGAMMFSSTIIGLKLLPTTILHHQHTGEVMISVLLMQDVIA
                     IVLLILLNSAHQDGNLSINDFILVIIALPALCLFSFLFERYILIKLLARFDRTQEYVF
                     LLSIGWCLGISVLAQTIGLSEDIGAFIAGVALASSPISLFIAESLKPLRDFFLVMFFF
                     SIGATFNFGFGAQVVIPAVILATLILIIKPLLFYILLARSGEKKQVAKEVGIRLGQGS
                     EFSLLVASIALSANLISEVASNLIQATTILTFIVSSYLVVLKYPTPIALSEKMRKD"
     gene            complement(8843..9664)
                     /locus_tag="AC06IO_002168"
     CDS             complement(8843..9664)
                     /locus_tag="AC06IO_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIO9875344.1"
                     /translation="MRKLLIVFSIFLASCQTTHDKPNLTNKKRQATHTKNSVYDRYKY
                     KSNRYTILQDGAPKQAKQVSFKEPVPVKEPLSRYGNPSEYYVDGRTYEVMKSSTGYKT
                     RGIASWYGTKFHKQRTSSGEPYNMYVMTAAHKTLPLPTYVKVRNLENGKVAIVKVNDR
                     GPFHADRVIDLSYAAALKLGVFPKGTAHVEIETLMGPAGQGHYYLQAGAFSSEALAKR
                     LKEKLVHITPSPVFIEHYKQHYIVRVGPFGNKSMADNLKTKLSNNGIKGSFSVLI"
     gene            9860..11083
                     /locus_tag="AC06IO_002169"
     CDS             9860..11083
                     /locus_tag="AC06IO_002169"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIO9875345.1"
                     /translation="MTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQKPSPTVTT
                     KPFTTPAAPLLNAKAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNALKSGQ
                     IHLNDMVRVSRDAWQTGGSRMFIKEGDQVSVEDLLKGIIVDSGNDACVAMAEHLGGSE
                     NAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWYK
                     QKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEP
                     SDSVRADDSEKLLNYGFRFFETHQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIP
                     AGQYERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKPLYALQTVESGGFYTR
                     TKDSIRLAFKRWFGS"
     gene            11096..>11708
                     /locus_tag="AC06IO_002170"
     CDS             11096..>11708
                     /locus_tag="AC06IO_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIO9875346.1"
                     /translation="MNIAFVNGKYCCQSEAKISIFDRGFLFGDSVYEVLPVYHGQPYF
                     VDQHLDRLFSNMKKIKMTIPNYDWHGLIHRLISENNGGNLQVYIQVTRGNQGMRKHDI
                     PTSITPSVIAFTMHNSFPTLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAV
                     SAGFHTAILARNGLITEGSSTNVFIVAQDGVIKTPPMNNFCLPG"
BASE COUNT         3489 a         2322 c         2123 g         3774 t
ORIGIN      
        1 attactaatc caaaatactg cacttaataa ttccttagtc ttttatttag accctttgca
       61 tgactgtcct ttcatttttt ttgctgtgct gcagatattt ttgtgggatg tatgtaaact
      121 ctaccccgca aaaagtttgt atcttatcca aaataaaata atcagcccat gcaagaggtc
      181 tatatataat tatatacata taataagcat ccctttgctt gtatgagcaa aggaaatgca
      241 atgaggtata attggctacc tgtaattatt attttttctt ctttttgggc aaatacagat
      301 ccgttatagt gccttcaaat gcttctgctg actgagctat ggtttctgat agagtggggt
      361 gaggatgaat agtaagagca atgtcttcaa catcacaaca catttcaatg gctaaggcag
      421 tttctgcaat taaatcgccg gcattaaccc caacaatacc tgctcccagg atacggttag
      481 tttctgggca gaatagtaat ttggtcattc cctcttctcg ccccatgctg agtgcacgac
      541 cgctagcagc ccatggaaag gaggctttct catagtttat tcctttctct tttgcttctt
      601 tttctgttag accagcccaa gctaattctg gatctgtata agcaacacta ggtatacatt
      661 ttggctcaaa ataatgtttt ttccctgcaa tgacttctgc tgcaactttt ccttcaggga
      721 ttgctttatg tgcgagcatt ggttgtccat tgacatcgcc aatagcaaaa atatgaggaa
      781 cgttcgttct taattgattg tcaactggaa taaagccacg ttcatctact ctgatccctg
      841 ctttttcagc gttgattgct ccaccgttag gtttcctgcc tactgcaaca agtacttgct
      901 gaaagcaaag tggcttatca gttgcatgtt ctccttccat tgaaacataa ataccatctt
      961 ttttggcttc aactgcagtt actttcgttt ttaatagaaa tttgacaccc tttttagtca
     1021 ttcgtttttg cagtatattg accaggtcgg catcagcatt cggaattaat tggtccataa
     1081 attcaacaac tgttacatta actcccagcg aggagtagac agttgccatt tccaagccaa
     1141 taataccacc acccaataca agtaagtcac ctttgatatc agcgagttct aatgctcctg
     1201 tagagctaaa tattctttta tcttctggta taaatggtaa tttaatggat tctgagccta
     1261 ctgcaataat ggcattatca aattcaattt ctacagtccc ttctttcgtt tcaacaagaa
     1321 tttgatgggt acctgaaaac ttacctgtcc cagtaattac ttctactttt ctttgtttgg
     1381 caagagcttt tagtcccccg gtcaactttg caacgacaga gtttttccag gcaaccaatt
     1441 ttttattgtc gaatttaggt ttaccaaatg ctacaccttg ctctgacatt tcatgggctt
     1501 catctactac tttagcaata tgcaataatg cttttgaggg aatgcaacca acatttaaac
     1561 aaacaccacc tagtgagtca aaacgctcga ccaaaaccac ctttttatcc aagtcggcag
     1621 ccctgaaagc ggctgtgtat ccgcctggtc cactacctaa taccactaca tctgtcttaa
     1681 ttttattagc cataagaagc ctcattaaaa aatatgtttt ctggggttat tggcatttca
     1741 ttcagaacaa ggaaatacca atagctctat tcctttatat gtcagagtaa aactcttcta
     1801 atatcaccta aacaatcaca aagaaagcga gtaaaccggg ctgcctcagc gccgtcgatt
     1861 actctatgat cgtaagaaag ggaaataggt aacatcaatc tgggcttaaa ctctttatta
     1921 tcgtatatgg gttttattat tgatcttgat aagcccaaaa ttgcaacttc cgggctattt
     1981 acaataggag taaatgccgt tccgcctata ccgccaaggc tggaaattgt aaaacaaccg
     2041 cctgacatat caataggtgt taatcccttt tctctagctt tcgtactgag tctggacatt
     2101 tcctttgcaa tatcaattac actcaattta tcaacattct ttattacagg aaccaccaga
     2161 ccgttaggtg tatctactgc tatacctata ttataatatt ttttataaat taaattttca
     2221 ccactggcat ccagtgacgc attaaattgc gggtaagcat ggagagcctt acaaactacg
     2281 ctgcaaacaa atgctaacag ggttaatttg taatcttgat ttttagcgct ctctgattct
     2341 gattttctaa acgcttccag gtctgtaata tctgcttcgt caaattgagt gacatgtgga
     2401 atggttatcc aggagcgatg tacgtttgtt ccagtaagtt ttttaatttt atttaaaggc
     2461 tttgtttcaa ttgaaccaaa tttactgaaa tcgatggctg gatttgacgg taagctgaag
     2521 ccaccaggcg tagttttttc acttaaacgt actttgatat aattttgcag atcttctttt
     2581 gtaatacgag attttctccc acttccctga accaaagata aatcgactcc aaactctctt
     2641 gccaaccttc ttacggctgg acctgcagat attaagatac tttttgattc agctatctca
     2701 aggctattaa ttgatattgg ttcggatttt agttcttcat atggcttctc tatttccttt
     2761 atagattgtt ctgagatatt tttaatctgg cttatctcga tttcaggtgt ctcagatttt
     2821 cctggggttt ttagagttag aataggagta ccttgagata ctttatcccc taatttaatt
     2881 ttcatttcga tgactttacc tgcatagggc gaaggaatat ccatcgttgc tttatcaccc
     2941 tctaatgtaa ttaatgcctg atccttttct acttccatcc ctggtttgac taaaatatca
     3001 attacatcaa cgtcatttgc accaccaata tccgggatag atatttcaat ttctttggaa
     3061 ccaggtatag atgcagttgc ctttgtatca acagattgct cttctgctct ggtttgcaaa
     3121 ttctgttttt ctggttcagc cttttgttct tgagaactgg aaatatttgt cgttgtatct
     3181 gacttggctt tgacaatgag atcgccttcg gatactttgt caccgacttt aacgagaatt
     3241 tgcgttaccg ttccagatat tggagaaggg atgtccatgc ttgccttctc agactcaagc
     3301 gtaatcaatg gtgtatctac ttcgatctga tcaccctctt tcactaaaat ctcaatgaca
     3361 tcaacttgat tagcaccacc gatatcagga attttaattt cactttcttt tgtcatattt
     3421 tcctcagcac tcagttaaac cggaactatc ttctgttctg attaattgtt taactaatgg
     3481 gtcattggat ccaatttgtc ttgattaata ttgtaacgtt tcatggcatc gacaattttt
     3541 gctttatcaa gactaccttc agctacaagt gcatttaatg ccgccaacac tataaatttt
     3601 gcatcaactt caaagaaatg acgtagttgt gtacgggtat cacttctgcc gtaaccgtct
     3661 gtacctaggg taatatatcg attaggaaca aaaggcctga tctgatctgc ataaatacgc
     3721 atatagtcag ttgaggcaat aacaggtcca cgacgtcctt ttaattgaga tgtcacataa
     3781 ctttcctgag gcttattttg ggggtgcatg ttattgtatc gctctacagc caaaccttct
     3841 ttacgtagct cattgaagct agttacactc cagatatccg atgtaattga aaaatcctct
     3901 tccaacatct ctgcggcttt gataacttca cgcaaaatag taccgcatcc cattagttgg
     3961 acatggtttt ttgatttttt cttattttct tttaagagat acattccttt gataatacct
     4021 tcctctactc ctgcaggcat atcgggatga gagtagtttt cattcataat agtaatgtaa
     4081 taaaacacgt tttcctgttt ttcatacata cggtgcaatc cattttggat gataactgct
     4141 aattcatagg cgtatgtggg atcataagaa atacaattgg gtattgttga tgctaagaca
     4201 tgactatgtc catcttggtg ttgcaatcct tctcctgcca acgttgttcg tccagcggtt
     4261 ccaccgagca gaaatcctct cgcttgcata tctcctgccg cccatgctaa atcaccaatg
     4321 cgttggaaac caaacataga gtaataaatg taaaatggaa tcattgccag cttatttgaa
     4381 ctgtaagagg ttgcggcagc aatccacgaa caaaacgccc cagcttcatt aatgccttct
     4441 tcaagaatct gcccgtcaac tgcttctcgg taatacatca cttgttcatg atctacggga
     4501 gtataaagtt gacctacggg agaataaatt ccaatttgtc tgaataatcc ttccataccg
     4561 aatgttctgc attcatctgg tactatgggg actattctgg aattaatgtc tttatttttg
     4621 agtagaactg aaagaattcg cacaaatgcc atagtagtcg agatttctct atctcctaat
     4681 cccttggtaa tactggaaaa ctctcctaaa tcaggtattt ttaactgctc tacttcagtt
     4741 gaacggtgtg gcaaataacc gcccaaggct tccctttgct tttttatata cttgatttcg
     4801 ggactgtcgt catcaggttt atagaaagga atatcagcaa ttttgtcatc gctgattgga
     4861 atattaaatc gatctctgaa agcttttagt tgatctatgg tcattttttt ctgttgatga
     4921 gtaatgtttt gtccctcacc agcagctccc atcccgtagc ccttgatggt ttttgcaagg
     4981 ataactgttg gagttccctt atgttcaacc gctctggcat aagctgcata tactttttga
     5041 ggatcatggc caccacgatt taatcgccaa atttcctcat cagacatatt ttcaaccatt
     5101 ttctttaatt ctggatattg attaaagaaa tgttgtctta catacgatcc atcgttcgct
     5161 ttgtaggatt gataatctcc atccagacat tcttccatcc ttttctgtag ccagccttga
     5221 ttatcacggg caaataatgg atcccaacgg cctccccaga tgaccttaat aacgttccat
     5281 ccagctcctc gaaataggcc ttcaagttct tgaatgattt taccgttacc acgcacaggc
     5341 ccatcaagtc tttgtaaatt gcaattgact acaaaaatga gattatccag cttttctcgt
     5401 gctgcgatac tgagtgcccc gactgattca ggctcatcca tctcgccatc acccaaaaat
     5461 gcccatactt ttctaccctc agctttgatc agtccccggt tctcaagata ttttagaaag
     5521 cgagcttgat agatagcttg caaagggcct aagcccatgg atacagtggg aaattgccaa
     5581 aaatcgttca ttagccaagg gtgtgggtag gaagataaac catctacctc tacttcttgc
     5641 cggaattttt caagttgttt ctctgttaat cggccttcaa gaaatgctct ggcataaatg
     5701 cctggagccg agtggccttg aatataaagc aagtcccctc cgttttcacc tttctgtccc
     5761 ttaaaaaaat agttaaatcc agtttcatat aacgttgatg cagatgcata agaagcgata
     5821 tggcctccaa gctctggcgc gtatttacct gccctaagta ccattgcaac agcgttccat
     5881 ctgattaatg cattgatgcg ctttccaata ccctcatcgg gaggcatttg tttttcttcg
     5941 tgaggtttta tagtgtttct gtagggagta ttaattgagc tggttagttt aaccccctcg
     6001 gcgttcgctt tattaagaag ctgctgcaaa aggaatgcag tacgttcagg accatcatta
     6061 aagagtacag cttgtagggc atccagccat tcacgcgttt ctattggatc cagatctaaa
     6121 tttgtttcat tggtcatgaa atagttcccc aacaatcaat aattttatga acagtatggt
     6181 acgggttgta attttttctg aattacacca gaacaacctt gattacatgc attaaaatcc
     6241 gcagagcaat tacaagttac tttgcggcat tgtagtggat cacggtaaga ttgcaacccg
     6301 cgagtaatat atccaccttg gattttgact tcgtgaaggt attccagata attttctttg
     6361 gcgaaatggt ctactttcgt agaacaatct cggcagttgt ctgtgcaatt ttgtttgcag
     6421 taattaagct gctgaaaaca agtttgttgg cattggctta aggttttatg atgggtaatt
     6481 ttacttttta gtgtggcaca agctgaaagc tccaatatta taaggcaaaa aagtaaacgg
     6541 aatgcagtgt tcatgattca agtcccacat taatttattg tgaaatagaa cattatagcc
     6601 aagctactaa cgtaaataat gcaataaaaa ccaacaacac tattgtggca tgttctacaa
     6661 gattttcagc agcagccatt ggtacttctt ctgtatcatt gatacgaact gcttgcaaac
     6721 cacatcctct cagtatttta tcatttgagt caggagaagt tagaacatat tgaacgaata
     6781 gatgaaaacc tcgttgaaaa tttccaacta acaaaaacag caaagcggtg attctggctg
     6841 gtatccattc gagaatgcta gtaatttgac ttgcctgagt acttataaaa ttaatttctt
     6901 tacataaggc gattaatctg tatgttaaag cggctattgg cccagcgatg atgtaccaga
     6961 atacaacagc aaataattgg ctgttaacct cagcaaaata ttctccaatt gcatctgttt
     7021 cagtttgatt agtctgtttc ttcaaaattg gataaaaagc attttgcggc ccaagacagt
     7081 agtaaaaaat aaacaggcta agaattaggc caaataaacc aaacagaata ctttgtagtg
     7141 aaaagtaaac aagaaatgtg agaaaaacaa tgggcaatat gatagctatc aaggtagccc
     7201 aaggattaga aaaatattca tttttatcga taaatttttt aagaaattgg cagtagttat
     7261 taaaccagga aaatcgctga taggacactg aatggattaa aaaccgctca ctgagcaagc
     7321 ataatactat taccagtaat ttcatttgat taatccttac gcattttttc agataaagct
     7381 ataggtgttg gatatttcaa tacaacaaga tacgatgaaa cgataaaagt taaaatagta
     7441 gttgcctgga ttaaattgga ggcgacttct gaaatcagat ttgcactcaa ggcgatgctc
     7501 gcaaccaata aagaaaactc actcccctgc cccaagcgaa ttcctacttc ttttgccact
     7561 tgctttttct caccagaacg ggcaagcaat atataaaata gcaacggctt aataatcagt
     7621 atcaatgttg ctaatatcac cgccgggata actacctgag ctccaaatcc aaaattaaaa
     7681 gtagcaccaa tcgaaaaaaa gaacatgaca aggaaaaaat ctcttaatgg ctttaaactt
     7741 tcagcaataa acaatgaaat tgggcttgat gccaaagcta ctcctgctat aaaagcgcca
     7801 atatcttctg ataagcctat tgtttgtgct aaaacggata tccccaaaca ccatcctatg
     7861 gagagtagaa aaacgtattc ctgagttcta tcaaaacgtg ccagcagttt tatcagtatg
     7921 tatctttcaa ataaaaaaga gaacaagcac agggccggta gcgcgataat aaccaggatg
     7981 aaatcattga ttgacaggtt cccatcctga tgggcactat taagtaatat caaaagcact
     8041 atggctataa cgtcctgcat taggagtacg ctaatcatga cttcaccagt atgctggtga
     8101 tgtaaaatgg ttgttggtaa taatttcaag ccaataatag tacttgaaaa catcatggct
     8161 ccgcccaata tcaaggactc agtcaccgtg aggccaaacc atctgccaat tccgtaggca
     8221 agtgcagcaa acagaattga gcttacgatt gcaatccagg ttacttttct tagcatatga
     8281 atcaaattct ggggctgtaa atgcaaacct aataagaaaa gcaagaatac tattccgatg
     8341 tcacctatct gcttgacaac cgtcacatcg gatacaagtt tgaatcccca aggacctaag
     8401 atagcaccaa gtgttatata agcaaccaat aatgactgtt tggtgtacaa taccaaggta
     8461 gaaaaaaaag cagctccagc aaatataaga aatatagtgt aaaatacaga gccatcatgc
     8521 attgattgaa tacctcttaa gaaaaggtag ttaaaattga attataaagc caattttatc
     8581 cattaatcta ttaattagct attaattatt gtttattaat aataaattgt tgcctttagt
     8641 tagaaccagg gtgatatcta ttgatgagaa tgtcatcgat agagtcataa tgtgctggta
     8701 aaattaacac aagctatgca cacattcaga atcagtctga gtataatgtt acacctaaaa
     8761 ctatttcttt cccaaactat tctgagtggt ttttttctta attctaaaga tataaattaa
     8821 ccggtttttc ccacgaaaca aattaaatta atacagaaaa agatcccttt attccattat
     8881 ttgataattt tgttttaagg ttgtcagcca ttgatttgtt accaaaaggt cctactcgga
     8941 caatatagtg ctgtttgtag tgttcaataa acaccggcga cggtgtaatg tgaactagtt
     9001 tctctttcaa acgctttgcc agtgcttctg aagaaaaggc tcctgcctgc aaataataat
     9061 ggccttgccc agcaggcccc attagcgttt caatttcaac atgagcagtt cctttgggaa
     9121 aaacccccag ttttaatgcg gcagcgtacg agagatctat gacacgatct gcgtgaaatg
     9181 gtcctctgtc attaacctta actattgcga ctttgccatt ttccaggttt cttactttga
     9241 cataagtcgg caaaggtaat gttttgtggg cagccgtcat gacatacatg ttgtagggtt
     9301 caccactgga ggtacgctgt ttatgaaatt ttgttccata ccatgaggca ataccacgag
     9361 ttttataccc ggttgagctt ttcatgactt cataagtacg cccgtcaaca taatattcag
     9421 aaggattacc atatctgctt aatggttctt tgacaggcac tggttcttta aagcttacct
     9481 gtttggcttg tttgggagct ccatcctgga gtattgtata acgattggac ttgtatttat
     9541 atcgatcgta gacagagttt ttagtgtgag tggcttggcg ttttttattt gttaggttgg
     9601 gtttgtcatg agtagtttgg cagcttgcca aaaaaattga aaaaacaata agtagtttac
     9661 gcatattctt tgatatttct tgttgttatg agttattact aatattacca aaaaataata
     9721 attttcgaag tgcgattaaa ttaattcaca acttatttag ctttataaaa aaaatattaa
     9781 tatttttaat atgccatttg gccgcctgca aaaggctgtg ataagataca tttattttgc
     9841 aactatatca ggtaatatca tgaccagagc aacgtctttt ttattgacgc tattattttt
     9901 tattacttta tttgtacagt ctaccaattc aatggctgat gaagtgcttc cctcctctga
     9961 tttgcagaag ccttctccaa cagtcacaac gaaacctttt acaactccgg ctgctccatt
    10021 gcttaatgcc aaagcttata ttttaattga tgtgaatagt ggaaaagtaa ttgcagaaaa
    10081 gaatagtgag gaaaaattac cacctgccag tttgaccaaa atgatgacgc tgtatgtcat
    10141 ttctaatgca ttgaagagcg gacaaatcca tcttaatgat atggtaagag taagccgtga
    10201 tgcatggcag acaggtggct ctcgtatgtt tataaaagag ggagatcagg tctctgttga
    10261 agacctttta aaaggcatta tagtagactc tggtaatgac gcttgtgtgg caatggcaga
    10321 gcatcttggt ggttccgaaa atgcttttac cgacattatg aatcagcaag ctcaaaatct
    10381 gggtatgaaa aatagtcatt ttacagacag tactggattg cctgatccta atctatatac
    10441 aactgctaaa gatttggcga ttttaggccg agccttaata ctcgatttcc ctgaatacta
    10501 tgactggtat aaacaaaaat ggtttacata caacggtata cgccaaccta atcgaaatcg
    10561 tttgttgtgg cgagatagcc aagttgatgg tataaaaaca ggtcacacca atgatgctgg
    10621 atattgtcta gtatcttctg caaagcgtaa taatatgcgt ttattagctg tggttttagg
    10681 ggaacccagc gattctgtgc gtgctgatga cagtgaaaaa ttattaaatt atggatttag
    10741 attttttgaa acacatcaac tatataaatc tgggcaatca attgctgaat taccgcttta
    10801 caaaggaact tctgataaag ttgctgtggg tttaacagaa gatcaattta ttacgatacc
    10861 tgccggtcaa tatgaaagat taaatatttc aactaaaatt tctaatgatt tacaagctcc
    10921 tataaagaaa ggcgataaag ttggcgatat tattgtgcaa tttaataaca atatcgtttc
    10981 aagcaaaccg ctttatgctt tgcaaacagt ggagtcaggt ggattctata ccagaacaaa
    11041 agattcgata aggttagcat ttaagcggtg gttcggctcc taggatattt tcataatgaa
    11101 tatcgcattt gttaacggca agtattgttg tcaatctgaa gcaaaaattt caatatttga
    11161 tcgaggtttt ctttttggtg actcggttta tgaagtgctg cctgtttacc atgggcagcc
    11221 ttactttgta gatcaacatc ttgaccgatt attttcaaat atgaaaaaaa ttaagatgac
    11281 tataccaaat tatgattggc atggtttaat tcatagacta atatcagaaa ataatggcgg
    11341 taatttacaa gtatatatcc aagtcacacg aggaaatcaa gggatgcgca agcatgatat
    11401 ccctacttcc atcacacctt ctgttatcgc attcactatg cataattcat ttcccaccct
    11461 tgaagataag gaacagggaa tgtcagcaaa actggttgaa gattttcggt ggatgagatg
    11521 tgatataaaa actacttctt taattgccaa tatattactg aatgatgagg ctgtatctgc
    11581 aggattccac actgcaattc ttgcccggaa cggtctaatt acagagggaa gtagtaccaa
    11641 cgtattcatt gttgcacagg atggtgttat taagacccca cccatgaata atttctgttt
    11701 accaggaa
//
LOCUS       DBNCWK010000099        11621 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.099, whole genome shotgun sequence.
ACCESSION   DBNCWK010000099 DBNCWK010000000
VERSION     DBNCWK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.099"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..5753
                     /locus_tag="AC06IO_002171"
     CDS             <1..5753
                     /locus_tag="AC06IO_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SidE phosphodiesterase domain-containing
                     protein"
                     /protein_id="HIO9875347.1"
                     /translation="VEGVELTQEGMHAIFARMGHPEIKSGTIYNGVPTIDQEALDKQG
                     FMPVLTGVGPKRDSGHWIMLIKGAGNQYHLFDPMGKVSGEGYQDILATQLPKGSTLSV
                     IPNEPGLNMGLCGYWVASVGLKARAELNKDNPPNLETMGQITTDAMKDELTDNGYPKI
                     TGWLRAVSDKFPAGDPQPDAKALREATEKDLHINIPSSVPPVKDTSPKEIPVKPTAPQ
                     QPSVPVWNGFSLFTDDTVRTAAKYAYDNYLGKPYTGPVESTPANIGGQMVYRQVHGLA
                     HTLRTMAYAEIIVEEARKAKLRGETLGKFKDGRTIADVTPEELKKIMIAQAFFVTGRD
                     DEESAKNYAKYHEQSRDAFLKYVKENESTLIPDIFKDQEEVNFYARVIEDKNHDWDST
                     PAHVLINQGHMVDLVRVKQPPESFLQRYFSSMQRWIGTQATEAVFSIQRQFFHATYEA
                     VTGFDSDNKEPHLVVSGLGRYVIGEDGKPIREAPKQGEKEGELKVFSQTYKLKENERF
                     MRVDEFLKLPEIQSTFPGSGKHLEGGMPGMNYMDYWDRLNSRERARCENDVDFCLKQL
                     QTAHDKAKIDPLKEAFQSSKEKGKRQPNMDEIAAARIIQQIMANPDCIHDDHVSINGQ
                     KLEEKFFRDLLAKCDMAVVGSLLNDTDIKNIDTLMRHEKDTEFHSTDPQAVPVKIGDA
                     WENRIRTKDGDVTQMKHDLIFLMQNDAWYFSRVNAIAQNRDKGSSFKEVLFTALMTPL
                     TNKSLMDTSHAPAPKKVYRGLNLPEEFKNKLINQANVMIANTTDRLFTDHSSEAFKQI
                     KLNDFSQMSGRTCASTTTTYELTKAWDSNVVFEMIDPDGLLHPKQVGTHMAGSEDEFS
                     IYLPEDVALVPVKVTFDGKTTNGEDRCVFTLVAVKSPDFIPRHESGYAVEPFIKMQKE
                     KVTHALDAIEKDKGSYNIDEQLKSLRTEMVRQAKLPLREGVFDRLSHRFSLETSDNKI
                     SPERRDFLNQHVIPVLQECHIALRTNNMDMMQNALAKFPTDKQWSAFKSGEAVRAKAQ
                     MDVLKQQIEKKIMLQTQIIPALTECSEALDKQNVIEALEALNKLPAEKEIGKAKGIGQ
                     ELREQITGVKQELTGNLESLQRATTTPIVQDAEKMRVRYETLVTDVTKRVTDFEKIKP
                     ANLENYNKAIADLNNMQQELDLLRNEKIRMHTDKDKAVDFSDIEALEKRLQEAQPNLY
                     PSLVEGTTKGIQEIEKIPKSISFDDVKSMTSKINGYLETLEFIRNERVKKHGESTEPL
                     DMSDLDKLKNQLQGVSQHLAQILLNGAKNSLDKIKDPATLEKEAPYIKRCLDHFAELE
                     NTLDSSVKAMKQKEDFNIYKNSLIEKQEKAYPEMLQLQYKSEALITQLRNLCNIHHDN
                     LVKTREEQQQLLNKTGGILGGFFSQITTAISQAEAKKANEFARFKTELNNDKNDVSQL
                     IQFLVKKKPSELEENLGISKENAEQLHGLLKQLDTKATPIDKLQENTRLIDEISTKMG
                     SKPVSPEHVAATKAREYYQFLLYETTQKIGNFEKVKPLDPESYKKAMSDLNNVQEALQ
                     FLRSEKSRIHDDKEKAVEFSDIEALEKRVQNTQPKLLTALLDKTTRDISALVKIPRKL
                     TFEDIKSMTTKLNSYLETLELIRNERIKQHGESPELLDMSDLDSLKGQLQTFNQNLTG
                     MILNATKDGLDRINDRANFNEGEPYVKACLDLLTELEKTLDSSVKGMKQKEDISACRN
                     SLLDKQEKANSGMLDLQSKSKDLVTQLRDICKIHHGNLAEARRTKLHSLDNQEGGLLG
                     GLWSVTNKLGVTTDTVGIERMQIKMKEQALARFKTELNNDKYDTNQVIAFLANKKPSE
                     LEEGLGISKENAEELHGLLSKLTSKMTSKIEIEENTQLIDEISTKIGTEPVKLELAHT
                     VDEDESDTYRRESGYF"
     gene            6123..10613
                     /gene="sdeA"
                     /locus_tag="AC06IO_002172"
     CDS             6123..10613
                     /gene="sdeA"
                     /locus_tag="AC06IO_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SdeA"
                     /protein_id="HIO9875348.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGYGNITSGSIYNGRPTINTLAL
                     NNQGFMPVLTGVGPHRDSGHWIMLIKGPGNQYYLFDPLGKASGEGYQNILAAQLPMGS
                     TLSVIPNDSGLNMGLCGYWVASAGLRAHQTLNQHNPPTLLNLGQTITDEMRNELDHDG
                     YRKITGWLRAVADEFPEGDPQLDGKALRENTEKDLKIEIPTLVLPGKDTSPKEISVKP
                     TTPQDKSVPVWNGFSLYTDNTVKAAAQYAYDNYLGKQYTGPVESAPANFGGQMVYRQH
                     HGLSHTLRTMAYAELIVEEARKAKLRGETLGKFKDGRTIADVTPQELKKIMIAQAFFV
                     AGRDDEASDAKNYQKYHEQSRDAFLKYVKDNESTLIPDVFKDQEDVNFYARVIEDKSH
                     DWESTPAHVLINQGHMVDLVRVKQPPESFLQRYFSSMQRWIGTQATEAVFGIQRQFFH
                     ATYEVVAGFDSDNKEPHLVVSGLGRYVIGEDGQPIREAPKMGQKVGDLKVFPQTYKLK
                     ENERLMRVDEFLRLPEIQSTFPGSGKHLQGGMPGMNDMDYWNRLNSLNRARCENDVDF
                     CLKQLEKAHDKAKIEPIKEAFQSSKGKERRQPNVDEIAAARIIQQILANPDCIHDDHV
                     LINGQKLEQQFFRDLLAKCEMAVVGSLLNDTDMGNIDTLMRHEKDTEFHSTNPEAVPV
                     KIGEYWINDQNKYNSSGNITQKKHDLIFLMQNDAWYFSRVNAIAQNRDKGSTFKEVLI
                     TTLMTPLTSKALVDTSQAKPPTRLFRGLNLSEEFTKGLIDQANTMIANTTERLFTDHS
                     PEAFKQIKLNDLSKMSGRTNASTTTEIKLVKETWDSNVIFEMLDPDGLLHSKQVGRHG
                     EGTESEFSVYLPEDVALVPVKVTLDGKTQKGENRYVFTFVAVKSPDFTPRHESGYAVE
                     PFLRIQAAKLAEVKNSIEKTQVEPDPEKIINLQSELGVVQFTDLSLEYKRFISNTVGP
                     LLENCLSGIMESDAVTLNKALATFPSDKQWSAFNFEEARQAKRQMDAIKQMVGNKVVL
                     DALTQCQDAMEKQNIAGALDALKSIPPESTISRERREQIQSIRQDLESLQRAVITPVV
                     TDEKKVKERYDALIENTSKKITELETGKLPNLDAVKKGISNLSNLKQEVTVLRNEKIR
                     MHVGTDKVDFSDIEKLEQQIQVMDTKLTDAYLLEVTKQISALENAKPKNQTELKTKIA
                     AFLDRTADIETLRNDRIKKHGSSKDPLDLSDLDKLSGNLQRINQSIVSSLINTIRVSI
                     NQMEAKTFHMQEKEIQQNFELLTKLEKTLDKSKTSEKLREDIPKLNDLLVAKQKAYPQ
                     MVQLQLKSEVFVTQLREVCQANYDDLDKTRNARLRELDRLDREAGITRMVGNLLWGFT
                     NKVGLTTDERLDIRTKQQSLARFRTELFNDKIDTDELISNLARKRPSELQEGLGISPD
                     NAMDLYMVLTNLESKTTSPEKLEERMKAIDDISTKIGREPEHLKFVMVEEDESNKKTI
                     GF"
     gene            11034..>11621
                     /gene="pmtA"
                     /locus_tag="AC06IO_002173"
     CDS             11034..>11621
                     /gene="pmtA"
                     /locus_tag="AC06IO_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid N-methyltransferase PmtA"
                     /protein_id="HIO9875349.1"
                     /translation="MSLSSVKKVYNIYSSFYDVLFGSIFHQGRSLCTNKVNKNAGLNA
                     SVLELGVGTGLSLPFYRPDLNITGIDISEKMLEKAEKRIVKKKLTTHIDLKIMDAANL
                     EFPDNHFDFVVAMYVASVVPDIDAFLKEISRVCKPSGEIIFVNHFASEKPILRFLEKK
                     LSHIDNFVGFNSNFSIHSILNYKEFKLLETQKVNLF"
BASE COUNT         4062 a         2238 c         2290 g         3031 t
ORIGIN      
        1 acgtagaagg ggtagaatta actcaagaag gcatgcatgc tatttttgca cggatggggc
       61 atcccgagat taaaagcggt actatttata acggagtacc tactattgat caagaagctc
      121 tcgacaagca aggttttatg cctgtgttaa caggggtagg acctaaaaga gactccggtc
      181 actggatcat gttaatcaaa ggagcaggca accaatacca tctctttgat ccaatgggta
      241 aagtttcagg tgaaggctat caagacattt tagccactca attgcccaaa ggctccaccc
      301 tttctgttat ccccaatgag cccggcttaa acatggggct atgcgggtat tgggttgcct
      361 ctgttgggct aaaagctcgt gctgaactca acaaggataa tcctcccaat ttagaaacca
      421 tggggcaaat cactacagat gcaatgaagg atgaattaac tgacaatggt tatccgaaaa
      481 ttactggctg gttaagggct gtctctgata aatttccagc aggtgatcct caacctgatg
      541 ccaaagcatt aagagaggct acagaaaaag atttacatat taatatcccc tcctctgttc
      601 ctccagtgaa agatacttct cctaaggaga tccctgttaa acccactgct cctcaacaac
      661 catccgtacc cgtttggaat gggttttcat tattcaccga tgacaccgtt cgcactgccg
      721 caaaatacgc ctatgacaat tatctgggga aaccttatac tggcccagtg gaatcaacac
      781 cagccaatat tggcggacaa atggtatacc gacaagtcca tggccttgct catactttac
      841 ggacaatggc ttatgcagaa atcattgttg aagaagcacg taaagcaaaa ttacgagggg
      901 aaacattagg aaaattcaaa gatggccgta ctattgctga cgtcactcct gaagagttga
      961 aaaaaattat gattgctcag gctttcttcg ttacaggaag agacgatgag gagtctgcaa
     1021 aaaattatgc caagtaccat gagcaaagcc gggatgcttt cctaaaatat gtcaaagaaa
     1081 atgaatccac cttgattcct gatatcttca aagatcagga agaagttaat ttttatgctc
     1141 gagtaattga agataaaaat catgattggg attcaacccc agctcatgta ctgattaacc
     1201 agggtcatat ggtcgatttg gttcgggtaa agcaacctcc tgaatcattc ttacagcgtt
     1261 atttcagttc catgcaacgt tggataggca cccaggccac cgaagccgtt ttttcaatac
     1321 aaagacagtt ttttcatgcc acctatgaag cagtaaccgg ctttgactcc gataacaaag
     1381 aacctcatct ggttgtttcc ggactgggac gttatgtcat cggtgaggat ggtaagccca
     1441 taagagaagc acctaaacaa ggagaaaaag aaggagagtt aaaagttttc tcacaaactt
     1501 ataaattgaa agaaaacgaa cgctttatgc gagtcgatga gtttttaaaa cttcccgaaa
     1561 tccaaagtac tttccctggt tctggtaaac atttagaagg cggaatgccc ggtatgaatt
     1621 atatggatta ttgggataga ttaaactcca gagaaagagc acgatgtgaa aatgatgtcg
     1681 atttttgttt gaagcaatta caaacagctc atgacaaagc aaaaattgat cctctgaaag
     1741 aagcatttca atccagtaaa gagaaaggaa aaagacaacc caatatggat gaaattgcgg
     1801 ccgcccgcat cattcagcaa atcatggcca atcctgattg catccatgac gatcatgtgt
     1861 ccatcaatgg ccagaaactg gaagaaaaat tcttccgtga cttgttagcc aaatgcgata
     1921 tggctgttgt aggctcgctg ctcaatgaca ccgacatcaa aaatatcgat actttaatgc
     1981 gacatgagaa agacaccgaa tttcattcga cagatcccca agcagttcca gtgaaaattg
     2041 gagacgcctg ggaaaataga ataagaacga aagatggcga cgtcactcaa atgaagcatg
     2101 atttaatttt cctcatgcaa aatgacgcat ggtactttag ccgagtcaac gccatcgcac
     2161 aaaaccggga taaaggttcc tctttcaaag aagtgttgtt taccgcctta atgacgcctt
     2221 tgactaataa atcattaatg gatacatctc acgccccagc tccaaaaaaa gtgtatcgcg
     2281 gactaaatct tccagaagag tttaaaaata aattaatcaa tcaagccaat gtgatgattg
     2341 caaacacaac agatcgactc tttacagacc actcatcaga agccttcaaa caaatcaagt
     2401 taaatgattt tagccaaatg tctggcagaa cctgtgccag tacaaccaca acttatgaac
     2461 ttacaaaagc ctgggattcc aatgttgttt ttgaaatgat tgacccggat ggtttactgc
     2521 atccaaaaca agtaggaacc catatggctg ggtcagaaga tgaattttcc atctatttac
     2581 cggaagatgt tgctctggtc cctgtcaaag tcacttttga tgggaaaaca acaaacgggg
     2641 aagatcggtg tgtctttaca ctggtcgccg taaaaagccc tgactttatc cctcgccatg
     2701 aaagcggcta tgcggttgag cccttcataa aaatgcaaaa agagaaagtc acccatgcat
     2761 tggatgccat tgaaaaggat aaaggcagct ataacatcga cgaacaacta aaaagtctaa
     2821 gaacagaaat ggtcaggcaa gccaaattgc ctcttagaga aggggttttt gatagactct
     2881 ctcatcgttt ttctctggaa accagtgata acaaaatatc tccggaacgc agagattttc
     2941 ttaaccagca cgtcatccct gtattacagg aatgccatat tgctcttaga acaaacaata
     3001 tggacatgat gcaaaatgct ttggcaaaat tccctaccga taagcaatgg tctgctttta
     3061 aatcagggga agcagtcaga gccaaagctc aaatggatgt attaaagcaa cagattgaaa
     3121 agaaaatcat gctgcaaact caaattatcc cggctctcac ggaatgtagc gaagcgctgg
     3181 acaaacaaaa tgtcatagaa gcattagaag cgcttaataa actgcctgca gaaaaggaaa
     3241 taggtaaagc gaaggggata ggacaagaat taagagagca aatcacgggt gtcaaacaag
     3301 agctaaccgg aaatcttgaa tccttgcaac gtgcaaccac tactccaatt gtacaagatg
     3361 ccgaaaaaat gagagtacgg tatgaaaccc ttgtcacaga cgtaaccaaa cgagtcaccg
     3421 actttgaaaa aatcaaaccg gccaatcttg aaaactataa caaagccatt gctgacttaa
     3481 ataacatgca acaagaatta gaccttctgc gcaatgaaaa aattcgcatg catactgaca
     3541 aagacaaagc agtggacttt tctgacatag aggccttgga aaagagatta caagaagcac
     3601 agcccaattt atatccctcg cttgtggaag gaacaacaaa aggtattcaa gaaattgaaa
     3661 aaatacccaa gtcaatcagt tttgacgatg ttaaatccat gacatccaag attaatggtt
     3721 acctggaaac cttggaattc attcgtaatg aaagagttaa aaagcatggt gaatccactg
     3781 agccactgga tatgtctgat ctggataaat taaaaaatca actacaaggc gtgagtcagc
     3841 atttagccca aattttatta aatggagcta aaaactcact cgataaaatc aaggatccgg
     3901 ctacactcga aaaagaagcc ccttatataa aacgatgtct tgatcatttt gctgagcttg
     3961 aaaacacact ggatagttca gtaaaagcaa tgaaacaaaa agaagacttt aacatctata
     4021 aaaactctct aatagagaag caggagaaag cctatccaga aatgttgcaa ttgcaataca
     4081 agagtgaagc attgattaca caattacgta atctatgcaa tattcatcat gataatttgg
     4141 tcaaaacgag agaggaacaa caacagctac tgaacaagac cggaggaata ttaggcggtt
     4201 tctttagcca gataacgact gctataagtc aagccgaggc aaagaaagca aatgaatttg
     4261 caagatttaa aactgaatta aataatgaca aaaatgatgt gagtcaactt attcaatttc
     4321 tggtaaagaa aaaaccatct gaattggaag aaaatttggg catatcaaaa gaaaatgctg
     4381 agcaattaca tggactgtta aagcaactgg atacaaaagc tactccaata gataaattgc
     4441 aggaaaacac aagattaatt gatgaaatat caaccaaaat gggtagtaaa cctgtctcgc
     4501 cagaacatgt tgccgcaaca aaagcgagag aatactatca atttcttcta tatgaaacga
     4561 cacaaaaaat cggtaacttt gaaaaggtca aacctttgga tcctgaaagt tataaaaagg
     4621 ctatgtccga cttaaataac gtgcaagaag cattacaatt cctacgtagt gaaaaatccc
     4681 gaatacatga tgacaaagaa aaagcagtag aattctctga catagaagcc ttggaaaaac
     4741 gagtgcaaaa tactcaacct aaattactga cagcactcct agataaaaca accagagaca
     4801 tctcagcatt agtaaaaata cccaggaaat tgacctttga agatataaaa tcaatgacaa
     4861 ccaagcttaa cagttacctg gaaaccctgg aattgattcg taatgaaaga atcaaacaac
     4921 acggtgaatc ccctgaactt ctggatatgt ccgatttgga tagtttaaaa ggtcaattgc
     4981 aaacctttaa tcaaaatttg actggcatga tactgaatgc tactaaagat ggtctggata
     5041 gaatcaatga cagggctaac tttaatgaag gagaacctta cgtaaaagca tgtcttgatc
     5101 ttttaacaga acttgaaaaa acactggata gttcggtaaa aggaatgaaa caaaaagaag
     5161 acattagcgc ctgtagaaat tctctccttg ataaacagga aaaagccaat tccggaatgc
     5221 tggatttgca atccaagagt aaagatctgg ttacccaatt acgtgatatc tgtaaaattc
     5281 atcacggtaa tttagctgaa gcaagaagga ccaagcttca ctcacttgat aatcaggaag
     5341 gaggattatt agggggatta tggtctgtta ctaacaaact aggtgttacc acagacacag
     5401 taggtatcga gaggatgcaa ataaaaatga aggagcaagc actggcaaga tttaaaacag
     5461 aattgaataa tgacaaatac gatacgaatc aagtcattgc ctttttagca aataaaaaac
     5521 catcagagct ggaagaaggt ttaggtatat cgaaagaaaa tgccgaagaa ttacatggac
     5581 tgttaagtaa actcactagt aaaatgactt cgaaaatcga aattgaagaa aatacacagt
     5641 taattgatga aatttcaact aaaataggca ctgaacctgt aaaactcgaa ttggctcaca
     5701 cggtagatga agatgagagt gatacctatc gccgagaaag tggttacttt taagtgcttg
     5761 ttcgatggtg aaaaatcgaa gatgctagca gatgagaaga gaaagacatt gtgtttttat
     5821 tttctcattc tgttaactgc tttaaaacat atcattccta ataaagaaac actataaaaa
     5881 caggcaaact tcttttctgc ttaaactcat cccttcaatc ctttatttta ctgccctgct
     5941 ctttatacaa gagaaacaca tcccaatatt ttttaaatat caaattcaca atatgagcat
     6001 tttttttgca tttaatactt tcttaatact acacttctat aatatacata caatttgaaa
     6061 tatttatgtt catcaatgtg cgcttttgct cacttatgaa catcagtttg ggagaaatca
     6121 taatgcctaa gtatgtcgaa ggggtagaac taactcaaga aggtatgcat gctatttttg
     6181 cacgcatggg gtatggcaat attaccagcg gtagtattta caatggaaga ccgactatta
     6241 acacactagc cctgaacaac cagggattta tgcctgtgtt aactggggta gggccacata
     6301 gagactccgg acactggatt atgttaatca aaggtcccgg taaccaatac tatctctttg
     6361 acccactggg taaagcttcg ggtgaaggct atcaaaatat tttagcagca caattaccca
     6421 tgggatctac cctttcagtt attcctaatg attccggttt gaatatgggg ttatgcggct
     6481 attgggtcgc ctctgccggt ttaagagcac accagacatt aaatcaacat aatcccccta
     6541 ctttgttgaa tctaggacaa accattaccg atgaaatgcg taatgaactc gatcatgacg
     6601 gttatcgaaa aattactgga tggttacgtg ctgttgcgga tgagttccct gaaggtgacc
     6661 cacaattaga tgggaaagca ttaagagaaa acactgaaaa agatttaaaa atagaaattc
     6721 ctaccctggt tcttccggga aaagatacct cccctaaaga aatttctgtc aaacccacta
     6781 caccacaaga taaatctgtg cctgtttgga atgggttttc attatacact gataataccg
     6841 ttaaagctgc agcacaatat gcctatgaca attatttggg aaaacagtat accggaccag
     6901 ttgaatcagc acctgccaat tttggcggtc aaatggtgta cagacaacat catgggcttt
     6961 cccatacttt acggaccatg gcttatgctg aattgattgt tgaagaagca cgtaaagcca
     7021 aattacgagg tgaaacatta gggaaattca aagacggtcg tactattgct gatgttaccc
     7081 ctcaagaact aaaaaaaatc atgattgccc aggcattttt tgtagctgga cgtgatgatg
     7141 aagcatccga tgccaagaac taccaaaaat atcatgaaca aagccgggat gctttcttaa
     7201 aatatgtgaa agacaatgaa tccaccttga ttcctgatgt tttcaaagat caggaagatg
     7261 tcaattttta tgcccgagtg attgaagata aaagtcatga ttgggaatca actccagccc
     7321 atgtgctgat taaccagggc catatggtcg atttggtacg ggtcaagcaa cctcctgaat
     7381 cattcttaca acgctatttc agttccatgc aacgctggat aggtactcag gcaacggaag
     7441 ctgtttttgg aatacaaaga cagtttttcc atgccaccta tgaagtagta gcaggttttg
     7501 actccgataa caaagaacct catctggttg tttctggttt aggtcgttat gtcatcggtg
     7561 aagatggcca acccatcaga gaagcgccta aaatggggca aaaggtagga gatttaaaag
     7621 ttttccctca aacttataaa ttaaaagaaa atgagcgtct tatgcgcgtc gatgagtttt
     7681 taagactgcc tgaaatacaa agtaccttcc ctggttctgg caaacattta caaggtggaa
     7741 tgccaggtat gaacgacatg gattattgga atcgactgaa ttctttaaac agggcacgat
     7801 gcgaaaacga tgtcgacttt tgtttgaaac aattagaaaa agctcatgac aaggcgaaaa
     7861 ttgagcctat taaagaagct ttccaatcca gtaaagggaa agaaagaaga caacctaatg
     7921 tggatgaaat tgccgctgcc cgtatcattc agcaaatctt ggccaatcct gattgcatcc
     7981 atgatgatca tgtgctcatc aatggccaga aactggaaca acaattcttc cgcgatttgt
     8041 tagccaaatg tgaaatggct gttgtaggct cactgctcaa tgacaccgac atgggaaaca
     8101 ttgatacttt aatgcgacat gagaaagaca ccgagtttca ttctaccaat cctgaagcag
     8161 ttccggtgaa aattggagaa tattggatta atgaccaaaa taaatacaat agtagtggca
     8221 atatcaccca aaagaaacac gatttaattt tcctcatgca aaatgatgcg tggtatttta
     8281 gccgagtcaa tgccatcgca caaaataggg ataaaggctc cactttcaag gaagtgctga
     8341 ttactacttt aatgacgcct ttgactagta aagcactagt ggacacctca caagccaagc
     8401 cccctacaag actatttcgt ggattaaacc tgtcagaaga gtttaccaaa ggattaattg
     8461 atcaagcgaa tacaatgatt gccaatacga cagagcgtct attcaccgat cattcaccag
     8521 aagccttcaa acaaattaag ttaaatgatt taagtaaaat gtccggcaga accaatgcca
     8581 gtacaaccac ggagattaag cttgtaaaag aaacctggga ctccaatgtc atctttgaaa
     8641 tgcttgaccc ggatggtttg ctgcattcca agcaagtagg acgtcatggt gaaggcactg
     8701 aaagtgaatt ctccgtttat ttgccggagg atgttgccct ggtcccagtc aaggtcaccc
     8761 ttgatggtaa aacgcaaaaa ggagagaatc gatatgtctt tacctttgtt gccgtaaaaa
     8821 gccctgactt tacaccacgt catgaaagcg gctatgcggt tgaaccgttt ttaagaatac
     8881 aagcagcaaa gttagccgaa gtcaaaaatt ctattgaaaa gacccaggta gagcctgacc
     8941 cggagaaaat aatcaatttg caaagcgaat taggagtagt gcaatttaca gatttatccc
     9001 ttgaatataa aagatttatt agcaatacag tcggccccct gttagaaaat tgtcttagtg
     9061 gaatcatgga gagtgatgcg gtaaccttaa ataaagcatt agcaacattc ccatctgaca
     9121 agcaatggtc agccttcaat tttgaagaag caaggcaagc caaaagacaa atggatgcca
     9181 ttaagcaaat ggttggaaat aaagtggtgc ttgacgcctt gactcaatgc caggacgcca
     9241 tggaaaagca aaacattgct ggcgcattgg atgcgctcaa aagcatccca cctgagagta
     9301 caataagcag agagcgaaga gagcaaatcc agagcatcag gcaagacctg gaatcattgc
     9361 aacgtgctgt cattaccccg gtagtaacag atgaaaagaa agtaaaagaa cgctatgatg
     9421 ccctgataga aaacacatcg aagaaaatta ctgaacttga aacagggaaa ttacccaatc
     9481 ttgatgctgt caagaaaggc atatctaact tgagcaacct gaaacaagaa gtaaccgtat
     9541 tacgtaatga aaaaatacgg atgcacgttg gaactgataa agttgatttt tcagatattg
     9601 aaaaactgga gcaacaaata caggtcatgg ataccaaatt aaccgatgct tatttacttg
     9661 aagtaaccaa acaaatttca gcgcttgaaa atgcaaaacc caaaaatcag actgaattga
     9721 aaacaaaaat agcagcattc cttgacagaa ccgctgatat tgagaccttg cgtaatgatc
     9781 gcatcaaaaa acacggttca tcgaaagatc cgctcgacct ctctgatttg gataaattaa
     9841 gcggcaactt acagcgcatc aatcaatcca ttgtttcgtc tttgataaat actatccggg
     9901 tttctatcaa tcaaatggaa gcgaaaactt ttcacatgca agaaaaggaa atacaacaga
     9961 atttcgagtt actcacaaaa ctcgaaaaaa cattggataa gtcaaagaca tcagagaaac
    10021 tgagagagga cattcccaaa ctcaatgatt tgctggttgc aaaacaaaaa gcctatcctc
    10081 aaatggtaca actgcaactc aagagcgaag tcttcgtcac acaattacgt gaggtatgtc
    10141 aagctaacta tgacgatctg gataaaacaa gaaatgcaag attacgagaa ctggatcgac
    10201 tggacagaga agctggaata acaagaatgg tgggcaatct gttatggggg tttaccaaca
    10261 aagtaggctt aaccacagat gaacgacttg acatcaggac taaacaacaa tcgcttgcac
    10321 gattcaggac tgaattattc aatgataaga ttgatacgga tgaactaatc tctaatttag
    10381 caaggaaaag accttctgaa ctgcaagaag gattgggaat atctcctgac aatgcgatgg
    10441 atttatatat ggtcttaact aatttggagt ctaaaaccac ttctccagaa aaacttgaag
    10501 aaagaatgaa agcaatcgat gacatttcca ccaagatagg tagagagcct gagcatttga
    10561 aatttgtcat ggttgaagaa gatgaatcca ataaaaaaac tataggattt taattgacct
    10621 taacccaaat cgcatgaagt tctgaagtcg ggtcttattg acccgacttt ttgttatgga
    10681 tttaaaataa aagagaatat atccatctac tggcaacgaa atttcgacca gtttgcatac
    10741 catttaattt agaccttgat tcttctccct ggtagctaca tcgcttacaa aagagtcact
    10801 ttgactttgt aaattcaaaa tttttacatg agatcattga cttatttaag gcatatcttc
    10861 atcctttgga tcatttcgca ttgttggcgc agatattact aaagataact ttgagatgaa
    10921 attacctcag tttcaacagg agtgaacttc ttcattacaa tatacgaatt tataatccct
    10981 aaaagcaaat cgtttttgtt tttcgttttg tctttctaaa tggagtattg gttatgtcgc
    11041 tctcttcagt aaaaaaagtt tataatattt attcttcttt ttatgatgtc ctttttggat
    11101 ctatctttca tcaaggccgc tctctatgta ctaataaggt taataaaaat gctggcctga
    11161 atgcttctgt tttagagctt ggggttggca cagggttatc tttacccttt taccgtccag
    11221 atttgaacat aactggaatc gatatttcag aaaaaatgtt agaaaaggct gaaaaacgta
    11281 tcgtcaagaa aaaattaaca acccatattg atttaaaaat tatggatgcc gctaatttgg
    11341 aatttccaga taaccatttt gattttgttg ttgccatgta tgttgcttct gttgtccctg
    11401 atattgatgc ttttctaaaa gaaatctcgc gtgtttgtaa accatcaggt gaaataatat
    11461 ttgtgaatca ttttgcctct gaaaagccta tcctgcgttt ccttgaaaaa aaactgtcgc
    11521 atattgataa ttttgtaggg tttaactcga atttttctat ccattccatt ttaaattata
    11581 aagaatttaa attgctggaa actcaaaaag tcaatctttt t
//
LOCUS       DBNCWK010000100        11533 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.100, whole genome shotgun sequence.
ACCESSION   DBNCWK010000100 DBNCWK010000000
VERSION     DBNCWK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.100"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(95..610)
                     /locus_tag="AC06IO_002174"
     CDS             complement(95..610)
                     /locus_tag="AC06IO_002174"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIO9875350.1"
                     /translation="MESNNTQQSIKTHSSVIVLHDLSKDSLILTRRSQMLKKHPGEVC
                     FPGGFQEINDQDLYSTALRELNEELGVTSDRITLIRKLNIEKTLLGLVIHPWYASIDS
                     IFPYTMNPQEVSELILVPMPLVKNQKNYKEIRIEKEGKHIVTCQFIANEELIWGATAR
                     IMRQLASQYQV"
     gene            complement(597..1919)
                     /gene="pabB"
                     /locus_tag="AC06IO_002175"
     CDS             complement(597..1919)
                     /gene="pabB"
                     /locus_tag="AC06IO_002175"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - 4-aminobenzoate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HIO9875351.1"
                     /translation="MIDSTLITLDYHDFSSESYEKLSRLPGFVLLQSTDKSMGRYDIL
                     SAYPYERMVIKNNIHDKNLVLTKLKNKLKIERSEFDLPFQGGAIGYVSYDFGALLHNH
                     ELYPQKDLAHIPVLNFGFYDWAIIVDHHLKKATLFARNNHSSTKSVVDEIKSILNNQR
                     TKSIQFSVQSEFLPLISRDNYRSSFNAIQQALRAGRSYQVNFTQPFQTKFTGDPWDMY
                     QRVNKNNPVPYAAFIRTEEADILSFSPERFLLYDNGEMLTSPIKGTIHRSKDPVVDKK
                     LKEQLLKCPKNKAENVMIVDLLRNDLGKLAKAGSVKVMNLCHIQSFNAVHHLVSDIRA
                     LCKEDIHPIEAFFSCFPGGSITGAPKLETMKIISEQETYKREVYCGCIAYFSQHGRFD
                     ANIAIRTVTAKDNVLYLAAGGGIVIDSDWEDEYRECFTKITAVINGIK"
     gene            2100..3173
                     /gene="pdhA"
                     /locus_tag="AC06IO_002176"
     CDS             2100..3173
                     /gene="pdhA"
                     /locus_tag="AC06IO_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIO9875352.1"
                     /translation="MTTVAQFEITYTQYLNEQGKLVAELPPFADNQSILKELYKIMVL
                     TRTFDKKAIALQRTGKMGTYAPINGQEAISTAIGHALKPEDVLVPYYRDYAAQFQRGV
                     KMSEILAFWGGDERGSHYSNNAEDLPICVPIASQCLHAAGVAFAFKYRNQPRVAVVCI
                     GEGGTSEGDFYEAMNVAGAWKLPVVFVVNNNKWAISVPIEKQTGSQTIAQKAIAAGFP
                     GVQIDGNDIFAARQVIGDAIEKARQGGGPTLIEALTYRLCDHTTADDATRYQPSHEVE
                     EAKAKEPIVRFKHYLMQQNIWTSQDEEKLVIECSETVEKAVDEYLNTKPQPVSSIFDY
                     HYAELPEYLIEQRAIAMEEYSHA"
     gene            3166..4140
                     /locus_tag="AC06IO_002177"
     CDS             3166..4140
                     /locus_tag="AC06IO_002177"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIO9875353.1"
                     /translation="MPNITLVEAVTQALAYELAHDENVVVFGEDVGKNGGVFRATVGL
                     QDRFGENRVFDTPLAESMIAGLAVGMSIQGLKPVAEFQFMGFIYPAMNQIISHAARMR
                     NRTRSRLHCPLVYRAPFGGGIRAPEHHSESTEALFAHIPGLQVVIPSSPKRAYGLLLA
                     AIRNPDPVIFLEPKRIYRLVKQPVPDDGQALPLGKCFTLQQGDDLTLISWGASMHETL
                     QAAKQLTDEGISCDVIDVATIKPLDIETILSSVEKTGRCVIVHEGAKTCGVGAEISAQ
                     IMEHCMADLLAPVQRVTGYDTVMPYFQLEKQYIPSIARIKNTVMSIME"
     gene            4143..5255
                     /locus_tag="AC06IO_002178"
     CDS             4143..5255
                     /locus_tag="AC06IO_002178"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIO9875354.1"
                     /translation="MNIFNLPDLGEGLPDAEIHEWFVKEGDTVKADQPLVSMETAKAV
                     VDVPCPQSGTIAKLYGKPGDVIKTGEPLVAFVSTTEKPADKGTVVGNLEESTDVSEDN
                     FIIGSQRSSHRVKTTPAVRLLAKKLGVDLSSLKGSGDNGVITREDVQNQANINSQPPA
                     GFEPLRGVRRAMLNSMVQSHAEIVPVSIFDEADIHIWKPNTDITVRLIRAIIHASKKE
                     PALNAWFDTKHAARRCFKEVHLGIAMDNEEGLFVPVIHDAEKYSDSELRKIIDEFKES
                     VRNRAVSADKLKGATITLSNFGKFSGRFASPIIVPPMVAILAVGRLYKAPVINDEDKI
                     EAHKMLPLSLSFDHRAITGGEATRFLGAVIESLQQA"
     gene            5442..7646
                     /locus_tag="AC06IO_002179"
     CDS             5442..7646
                     /locus_tag="AC06IO_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIO9875355.1"
                     /translation="MAMRIFVMILLFFFSISPTLGDSTLPAIFDVKNANKQFDQINLQ
                     LSVQNLNLNNLNKAVETLGELSAQSDECIADIQKKINGVDVLIKQATSTGNDKGADLV
                     YLNNQKHQLAELQSQCRLFSIRAQEAIEAYKSAILQLRQEKTLARTLPLWQLASKIIN
                     SSDNTKWINHTKEKFLLQDLPVYLWFCIVAGSFFISILILSKLRQSNVIRRYVKLKKI
                     RWSHITLLALFMMTGSFYGYISLFTGEEQLINSQFLLIKELFWFLCGTLFIVCLFKVK
                     KIKAFFDWYAMDSEFFEILSIVIVAFYFLALSEQRLFDIWEVNKIIVELTRSIFLFLL
                     LGTTAYFTHYFCRSHLHISFVKRHYNFIRYFIFALLSGCAILNINGFYTLAMQLTLSG
                     IFTFFILYITILIGQSINKFYASLSYSQGKLNVIRIFGYKKDQMLTEFYILKVTLKLV
                     IVLLSVYLIGISWGFATDFIDTVYKKLLYGFTLANITIYPTRILFGIIIFCFLYLVSR
                     AISTSISRHQQFENEEETQVAIASILTYVGFSIAVISGLLVAGFNFTGLAIIAGALSV
                     GIGLGLQSIVNNFVSGIILLIEKPIRPGDRINIDGVEGFVKKIRVRSTQILTPVNEDI
                     IIPNSDLITRRVVNYMLTDNYWRVNCEVSVAFGTNLNLVKDILLEIANKHDDVVKSGR
                     NKPVVLFTSFADSALTFQLWCMIKDVNKKSTVKSELNFSIEEAFRKHDISIT"
     gene            7819..9963
                     /locus_tag="AC06IO_002180"
     CDS             7819..9963
                     /locus_tag="AC06IO_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9875356.1"
                     /translation="MIKKIHRWLPLLVLIGLLVLFFSLHLDKYLSFNALRENHALLIA
                     WTQTHYFIAPLIFIVFYTTAVAISIPGAVLLTLTGGFLFGVFWGVLFVVISATLGATI
                     LFFAVRTALGDWFAQKASGWIEHMRRGFQHNAFSYLVTLRLIPLFPFWAVNIVPALLN
                     IRAKTFVTATFLGIIPGTTVYVMVGNGLSQIFAANQTPNLGIIFELQILGPLLALAVL
                     SLMPVLYQFSTRKHQNHHSFSDISRVINCDLAIIGGGAGGLSLASGCSQLGLKVVLVE
                     SGKMGGDCLNYGCIPSKSLLAAAKTFYYAKHAAHFGVHTEAIKVNFEQVMQHVHQIID
                     NISEHDSVQRFESLGVQVIKQVGKFLTPDTLQAGDSIIKAKRFVIATGSSPFIPPIPG
                     LDAVSYFTNETIFDLKEQPEHLIVIGGGPIGCELAQAFAMLGSKVTLLEGLNLLPKDD
                     PDCVAVLRTQMKSMNILIYEQIEITQINSHPDKGISVCFEFQNTQFIITASHLLIATG
                     RRANVKPLDLEKAGVKLTSKGVEVNKYLQTSNKKIYALGDVTGLYQFTHMASYQAGIV
                     LRNIVFKLPSKVDYRAIPWVTYTDPELAHVGIGVSDALRHPDTQIIEWPFVDNDRAQT
                     EHTLNGKIKIITDKKARILGVTIVGPHAGELILPWVMAIREKKTLRSFTDVIVPYPTL
                     SEISKRVAGSFYAPKLFSNKTRMLVRWLQKIG"
     gene            complement(10019..10717)
                     /locus_tag="AC06IO_002181"
     CDS             complement(10019..10717)
                     /locus_tag="AC06IO_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875357.1"
                     /translation="MIHLLINWLIMRVIILNTILSLFLGVVGSLSHAAKSKNINQIDS
                     LFNIVKMSTVLSKDSLISPYNYLLTQPLMTTTLEKYYQRISKIKVITAFTNPHNNTYS
                     RIIILYVDRNKKRNNVDLAQTKNEIIPVELAAININFEELPEKLITDIIHTNIPFGKL
                     LTQYHLKVFSKDRRYFSVICNNMLASLIHCKPNKKLYGRTNVLARADNKKWVAHVIEI
                     LSDEKEVKHRLRLN"
     gene            complement(10795..11520)
                     /locus_tag="AC06IO_002182"
     CDS             complement(10795..11520)
                     /locus_tag="AC06IO_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIO9875358.1"
                     /translation="MENLEHGHSQKEIRDRLAQAPSQNYLRDWIYGGIDGVVTTFAIV
                     SGVVGGQLSSLVILILGFANLLADGFSMAASNYLGTKSEVDQFQRYKTIEEKHIDFIP
                     EGEKNEIKQIFQNKGLHGQTLDQVVEEITANRALWIKTMLQEEYGLPATLRFPLKSAL
                     YTFSAFLLFGIIPLIPYVLVMNNSFIWSCFFTAITFFVIGSIKSHWSTKSWLYSGFET
                     LIIGTVTASLSYGIGLFLHYFLT"
BASE COUNT         3529 a         2031 c         2205 g         3768 t
ORIGIN      
        1 atttgatttg tacaatttgt agcatacgct ttctctaatt tggtttaata atcaggtaaa
       61 ggcatttaaa gaaatgcctt ttatcatcct ggtattatac ttgatattga gaagctaatt
      121 gcctcataat tcttgctgtg gcaccccata ttaattcttc attagcaata aattggcagg
      181 tgacaatatg tttcccctct ttctcaattc taatttcttt ataatttttc tgatttttta
      241 ctaaaggcat aggcacaaga attaattcac taacctcttg tggattcata gtgtaaggaa
      301 atatagaatc aatactggcg taccaagggt gaatgactaa acccagtaaa gtcttttcaa
      361 tatttaattt tctgatcaga gttattctgt ctgatgtaac acctaactct tcattaagtt
      421 ctctcaatgc agtgctatat aggtcttgat catttatttc ttgaaaacca ccaggaaagc
      481 acacttcccc tggatgcttt ttaagcatct gacttcgcct ggtgagaatt aaggagtctt
      541 tggaaagatc atgtaaaacg ataactgatg aatgtgtttt gattgactgt tgggtattat
      601 ttgattccat tgataactgc agtaattttt gtgaaacatt cacgatattc atcttcccaa
      661 tcggagtcaa taactattcc ccctcctgct gccaaataca aaacattatc tttggctgtc
      721 accgtacgta tcgcgatatt ggcatcgaaa cggccatgtt gggaaaaata ggctatgcaa
      781 ccacaataga cctctctttt ataggtttcc tgttcactga taattttcat cgtctctaat
      841 ttaggcgccc cggtaattga accacctgga aaacaggaga aaaatgcttc aattggatga
      901 atatcttctt tgcatagagc tcgtatgtcg gaaaccaaat gatgtactgc attgaagctc
      961 tggatgtggc aaagattcat tacctttaca ctgccagcct tggcaagttt ccctagatcg
     1021 tttcttagca aatcgacaat catgacattt tcagccttgt ttttaggaca tttcaataac
     1081 tgttctttta attttttatc tacaacagga tctttcgatc ggtggatagt gccttttatg
     1141 ggcgaagtta acatttcacc gttgtcataa agtaaaaatc gctcgggaga aaagctcaat
     1201 atgtcagctt cctcagttct gataaaagca gcatagggga cagggttatt tttgttaacc
     1261 ctttggtaca tatcccaggg atccccggtg aattttgttt gaaacggctg cgtgaaatta
     1321 acctgataag agcgtcctgc tctcagggct tgttgaatgg cattgaatga gcttctgtaa
     1381 ttatctcttg atattaatgg caaaaattca gattgaactg aaaattgtat cgattttgtg
     1441 cgttgattat tcaatatact ctttatttca tctacaacgc ttttggttga agaatgattg
     1501 tttcttgcaa ataaagtggc cttttttaaa tggtgatcaa ctatgatagc ccaatcataa
     1561 aatccgaaat ttaaaacagg tatatgcgcc agatcttttt gtggatatag ttcatgatta
     1621 tgtaatagtg caccaaagtc gtatgaaacg tagcctatgg ctccgccctg aaaaggaaga
     1681 tcaaattctg accgctctat ttttagttta ttttttaatt ttgttaatac taaattttta
     1741 tcatgtatat tgttcttaat aaccatcctc tcatagggat aagcacttaa aatatcataa
     1801 cgccccatcg atttatccgt actctgaagc aggacaaatc caggtaaacg actgagtttt
     1861 tcataggatt cagatgaaaa atcatggtag tcaagtgtaa taagagtact atcaatcaaa
     1921 ttatcacctc aagataagga tgtgctgtga aaaaaatgta gtgtttttca tttggtcatt
     1981 ttaacctaac ttatcaataa aaaacgtata tttttatctc gggttcctat taatttactg
     2041 gtttttaaat taatttgtga gtatcctatg ccaacgcgtt gttgacaagg agccatgtaa
     2101 tgacaactgt tgctcaattt gaaataacat acacacaata cctgaatgaa caaggtaaat
     2161 tggtcgctga attacctcca ttcgcagata atcaatccat acttaaagaa ttgtataaaa
     2221 tcatggtact tacccgtact tttgataaaa aagcaattgc attacaaaga acagggaaaa
     2281 tgggaaccta tgcgccaatt aatggccagg aagctatttc gactgcaatt ggacatgctc
     2341 ttaaacctga agatgtactt gtcccttatt acagagatta tgctgctcaa tttcagagag
     2401 gcgttaaaat gtcagagata ctcgcctttt ggggcgggga tgagcgagga agtcattatt
     2461 ctaataatgc cgaagatttg cctatttgtg tcccaattgc atctcaatgt ttacatgccg
     2521 caggagtcgc atttgccttc aaatacagaa accaaccaag agtagctgtg gtatgtattg
     2581 gagaaggagg cacttcagaa ggtgattttt acgaagccat gaatgtggct ggggcatgga
     2641 aattaccagt ggtcttcgta gtaaataata acaaatgggc gatttctgtg cctatagaga
     2701 aacaaactgg ctcacaaaca atagcccaaa aagcgattgc tgctggtttt cctggcgttc
     2761 aaatagacgg taatgatatt tttgctgcaa gacaggttat aggggatgct attgaaaagg
     2821 ctcgtcaagg tggtggacct actctaatcg aagccttaac ctatcgcttg tgcgaccata
     2881 caacggctga tgatgcaaca cgctatcagc cttctcatga agtagaagag gcaaaagcca
     2941 aagagcctat tgttcgtttt aaacactatt taatgcaaca aaatatttgg acatctcagg
     3001 atgaagaaaa gctggttata gaatgcagtg aaacggtaga aaaagcagta gatgagtatt
     3061 tgaataccaa acctcagcct gttagcagca tttttgatta tcattatgca gaattaccag
     3121 aatatttaat tgaacaacgc gcaatagcca tggaggagta ttctcatgcc taatattact
     3181 ttagttgaag ctgttactca ggctttggcg tatgagttag ctcatgatga aaatgtagta
     3241 gtctttggcg aggatgttgg taaaaatggt ggagtatttc gagctactgt aggtttacaa
     3301 gacagattcg gagaaaatag agtttttgat actccactag cagaatcgat gatagctggc
     3361 cttgctgtag ggatgtctat tcaaggctta aaacctgttg ctgaatttca attcatgggt
     3421 tttatttatc cagccatgaa tcagatcata tctcatgcag cgcgtatgcg taacagaacc
     3481 agaagccgct tacattgccc tttggtttat cgtgctccat ttggaggtgg aatcagagca
     3541 ccggagcatc attctgaaag tacagaagca ttatttgctc atattccggg tttgcaagtg
     3601 gttattccct cttctccaaa acgcgcctat ggcctgttgt tagcagctat acgtaacccc
     3661 gatcccgtta tctttcttga accaaaacga atttatcgat tggtcaaaca acctgttcca
     3721 gatgatggac aagccttacc gttaggtaaa tgttttaccc tgcaacaggg agatgatctg
     3781 acattaatta gctggggagc cagtatgcat gaaaccttgc aggcagccaa acaattaact
     3841 gatgaaggta tttcctgcga tgttattgat gtagcaacaa ttaagcctct ggatatagaa
     3901 actatattaa gttctgtaga aaaaacaggg cgttgcgtca tagttcatga aggagcaaaa
     3961 acctgtggag tgggtgctga aatatcggct caaattatgg aacattgtat ggcagattta
     4021 ctagctcctg tgcaaagagt tacgggatat gatacggtta tgccttattt ccaacttgaa
     4081 aaacagtaca tacctagcat tgcacgtatt aaaaacactg ttatgagtat aatggagtaa
     4141 taatgaatat ttttaatcta cccgatttag gtgaaggttt acctgacgct gaaattcatg
     4201 aatggtttgt taaggaaggc gacacagtta aagcagatca acccttagtt tctatggaaa
     4261 cagcaaaagc agttgttgac gtaccctgcc ctcaatcagg cactatcgca aaactttatg
     4321 gcaaaccagg tgacgttatc aaaacaggtg agcctcttgt agcgtttgtt tcaactaccg
     4381 aaaaacctgc agataaaggc acagttgttg gtaatctgga ggaaagtact gatgtgtcag
     4441 aagataattt tattattgga agtcagcgtt cttctcaccg cgttaagaca acacctgctg
     4501 taagattgtt agcaaaaaaa ttaggagttg atttaagctc tttaaaaggt agtggtgaca
     4561 atggtgttat tactcgagaa gatgttcaaa atcaggccaa tatcaattca caaccacctg
     4621 ctgggtttga gcccctacga ggtgttaggc gagccatgct taatagcatg gtacaatctc
     4681 atgcagaaat agttccagtc agtatttttg atgaagcaga tatccatatt tggaaaccca
     4741 acacagacat tactgttcgt ttaatccgag ctattatcca tgcatcaaaa aaggaacctg
     4801 ctcttaatgc ctggtttgat accaaacatg ccgcacgtag atgctttaaa gaagtgcatc
     4861 ttggcattgc catggacaat gaagaaggtt tgtttgttcc tgttatccat gatgcagaaa
     4921 aatactccga cagtgaatta agaaaaataa ttgatgaatt caaagagtca gttcgtaatc
     4981 gggcggttag tgcagataag ctaaaagggg cgacaattac gttgtctaat tttggaaaat
     5041 tttcaggacg ttttgccagt ccaataattg ttcctccaat ggtagcgatt ttagcggttg
     5101 gaagacttta taaagcccca gtaattaatg atgaggataa aattgaagcc cacaaaatgc
     5161 ttcctttatc cttgagcttt gatcatagag ctattaccgg aggagaagcc actcgatttt
     5221 taggtgctgt tattgaatct ttgcaacaag catgatttta atttatgttg ggcttcgtaa
     5281 atagcgaggc ctaacaacaa atactattac tacaactatc tgtcttttct atataaattt
     5341 aaatgcgaaa aatttaatcc tagtgatatt ttaaatttcc ttcttgagat tcaatccata
     5401 agcaagtaga gttaatagtt tgttaattgg caaggaagag aatggccatg agaatttttg
     5461 tcatgatttt gcttttcttt tttagtattt ccccgacctt gggggatagc actttgcctg
     5521 caatttttga tgtcaaaaat gcaaataaac aatttgatca aatcaatctc cagttgtcag
     5581 tacaaaatct taatttaaat aacctgaata aggcagtaga aactctcggt gaactgtctg
     5641 cgcaatccga tgaatgtata gctgatatac agaaaaaaat aaatggagtg gatgtattaa
     5701 tcaaacaagc tacctctaca gggaatgata aaggagctga tttagtctat ctgaataatc
     5761 aaaagcatca gttagctgag ttacaatctc aatgtcgatt attctcaatt cgagctcagg
     5821 aagcaataga agcttataaa agtgctattt tgcaattaag gcaagaaaag actttggcgc
     5881 gtaccttacc attatggcaa ttagcaagta aaattattaa ttcatctgat aataccaaat
     5941 ggataaatca cactaaagag aaatttttac ttcaggattt gcctgtatat ctatggtttt
     6001 gtattgttgc aggaagcttt tttatctcta tcctgatctt gtcaaaatta cggcaatcca
     6061 atgttatacg ccgttatgtg aaattaaaaa aaattcgctg gtctcatatt acacttttgg
     6121 ctttgtttat gatgacgggt tcattttatg gctacatttc cctatttact ggtgaagagc
     6181 agctaatcaa ttctcaattt cttttaataa aggagctttt ttggtttctt tgcggcacat
     6241 tattcattgt ttgtttattc aaagtcaaaa aaatcaaagc tttttttgat tggtatgcta
     6301 tggatagtga attttttgaa atactaagca ttgtgattgt tgcattttat ttcctcgcat
     6361 tatcagaaca gagattattt gatatttggg aagtcaataa gattattgtg gagttaactc
     6421 gctccatctt tcttttttta ttgcttggta caaccgctta ttttactcac tatttctgcc
     6481 gttctcatct gcatatttct tttgtaaaaa gacattataa ttttattcgg tactttattt
     6541 ttgcactgct ttcagggtgc gcaattctga atattaatgg cttttatact ttagccatgc
     6601 agcttactct atctggtatt ttcacctttt ttatcttgta tatcactatt ttaataggac
     6661 agagtattaa taaattttat gcttcattaa gttactcaca aggcaaattg aatgttatac
     6721 gaatattcgg atataaaaag gatcagatgc ttactgagtt ttatattctt aaagttacct
     6781 taaaacttgt tattgtactc ctaagtgtct atctgatagg gatatcctgg gggtttgcta
     6841 ctgattttat tgatactgtt tataagaaat tattgtatgg ctttactctg gcaaatatta
     6901 caatttatcc aacgcgcatt ctcttcggga tcataatttt ttgtttttta tatttggtgt
     6961 ctcgtgccat ttcaacttca atcagtcgtc accaacagtt cgaaaacgaa gaagaaactc
     7021 aggtagctat tgcttccatt ttgacttatg ttggattcag tattgctgtt atttcaggct
     7081 tactcgtcgc gggctttaat tttactgggc ttgcgattat cgctggagca ctttctgtag
     7141 gaattggtct tggactgcaa agcatagtta ataattttgt atcaggtata attttgctta
     7201 ttgagaaacc aataagacct ggagatcgaa ttaatatcga tggtgttgaa ggatttgtaa
     7261 aaaaaatcag agtcagatca acacaaattc tcactccggt aaatgaagat attattattc
     7321 caaactctga tttaattacc cgccgtgttg tcaattatat gctgacagat aattattggc
     7381 gtgttaattg tgaggttagc gtggctttcg ggacaaacct gaatcttgtt aaagacatcc
     7441 tgttggagat cgctaataag catgatgatg ttgtaaaaag tggtagaaac aaacctgttg
     7501 tgttatttac cagttttgcc gacagtgcat taacttttca actctggtgc atgattaaag
     7561 atgtaaataa aaaatcgaca gtcaaaagtg aattaaattt ctctattgag gaggcttttc
     7621 gcaagcatga catttcaata acatagtctt aatgtgattt tcatatttga attggagatt
     7681 aaatactttc cctactctgg aaaataatgc ttattatcat taaaccctat cgataattaa
     7741 ggtatgacct caaagtaaat tcctgttagg atcttcatat aatttatatc agcttatcgg
     7801 gttaaaggaa taacatgaat gattaaaaaa atacatcgtt ggttaccttt gcttgtatta
     7861 atcgggctat tggtactttt cttcagcctg caccttgata aatacttaag ttttaatgca
     7921 ttaagagaaa atcatgcttt actcattgcg tggacccaaa cacattattt tattgcgcca
     7981 ctgattttta ttgtttttta taccactgct gtagccatct ctattccagg agcagttctg
     8041 cttactttaa ctggaggatt tttattcgga gtattctggg gcgtgttatt tgtagtcatc
     8101 agtgctacgc ttggggctac catactgttt tttgcggttc gcactgcttt gggcgactgg
     8161 tttgcccaaa aggcatctgg atggatagag catatgcgtc ggggatttca gcataatgct
     8221 ttttcttact tggttacact gcgattaatt ccattatttc ccttttgggc agtcaatatt
     8281 gtacctgctc ttttaaatat tcgagcaaaa acattcgtta cagcgacttt tcttggcatt
     8341 attccaggaa caacagtata tgtaatggtt ggaaatggtt taagccaaat atttgcagcc
     8401 aatcagacgc ctaatcttgg gattatattt gaactgcaaa tattaggtcc cctgctcgcg
     8461 ttggctgttc tctctctaat gccagttctt tatcaattct caactcgaaa gcatcagaat
     8521 catcacagtt tctcagatat tagtcgagta ataaattgtg atttagccat tataggtggt
     8581 ggagccggag gtttgagttt ggcttccggt tgttcacaat taggactcaa agttgttttg
     8641 gttgagtcag ggaaaatggg aggagactgt ttaaattacg gttgcatccc atccaaatcc
     8701 ttacttgctg cagccaagac gttttattat gcaaaacatg cagcacattt tggagtacat
     8761 actgaagcaa taaaagttaa ttttgaacaa gtaatgcaac atgtgcatca aatcatagac
     8821 aacatttctg aacatgattc tgtccaacgt tttgagtcgc taggagtaca ggttattaaa
     8881 caagttggga aatttttaac tcccgataca ttacaagctg gcgatagtat tattaaagcg
     8941 aagcgtttcg tgattgcaac aggttcatca ccatttatcc cacccattcc cggattggat
     9001 gcagtctctt acttcaccaa tgaaacgatt tttgatttaa aggaacaacc tgagcacttg
     9061 attgtcatcg gtgggggccc tattggttgt gaacttgctc aagcgttcgc catgcttggt
     9121 tctaaagtta ctctgctcga aggattgaat cttcttccca aggacgatcc tgattgtgtt
     9181 gctgtcctac gcacacagat gaaatcgatg aatatcctca tttatgagca aattgagatt
     9241 acccaaataa actctcaccc tgataagggt atttccgtat gttttgaatt tcaaaacaca
     9301 caattcataa ttacagccag tcatctgctc attgcaactg gacgtcgggc gaatgtaaag
     9361 ccacttgatt tggaaaaggc aggggttaaa ctcaccagca aaggtgtcga agtaaataaa
     9421 tacctacaaa ccagtaataa aaaaatctat gcactgggtg atgtaacagg gctttatcag
     9481 tttactcata tggcgagtta tcaggccggt atagtgcttc gtaatattgt ttttaaacta
     9541 ccgtctaaag tggattatag agcgattcca tgggtcactt atactgatcc cgaattagct
     9601 catgttggaa taggagtcag tgatgccttg aggcaccctg atacccaaat aatcgaatgg
     9661 ccatttgtcg ataatgatcg agcacaaact gagcatacat taaatggaaa aataaaaatc
     9721 atcacggaca aaaaagcaag gatccttgga gtcaccatag ttggcccaca tgccggagaa
     9781 ctcattttac cttgggttat ggcgattcgt gagaaaaaaa ctctccgaag ttttactgat
     9841 gtaatcgttc cttaccctac tctgagtgaa atcagcaaac gagttgctgg tagtttttat
     9901 gcacctaaat tattttccaa taaaacacga atgttagttc gctggttgca aaaaattgga
     9961 taaatagatt tttaactaac tatatgtttt ttagctgaaa gaaaaaatta caagctattt
    10021 aatttaatct taaacgatgt ttgacttctt tttcatcaga taaaatttca ataacatgag
    10081 caacccattt tttattgtct gctctagcaa gcacattggt gcggccatat aattttttat
    10141 ttggtttgca atggattagt gaagccagca tgttgttgca aataaccgag aaatagcgac
    10201 ggtctttact gaaaactttc agatgatatt gagttaataa ttttccaaaa gggatattag
    10261 tgtgaattat atcagtaata agtttctccg gcagttcttc aaaattaatg ttaatagctg
    10321 ctaattcaac tggaatgatt tcatttttcg tttgggcgag atctacatta tttcttttct
    10381 tatttcggtc tacatacagg atgataattc gagaataggt attattatgc ggatttgtaa
    10441 aggcagtaat tactttaatc ttggagatgc gctgatagta tttttccaaa gtggtcgtca
    10501 ttaatggttg tgtgagtaag taattgtaag gagagattag actgtctttg cttaatacag
    10561 tgctcatttt aacaatattg aacagactat ctatctggtt tatatttttg gatttagccg
    10621 catgagataa actccccacc actcctaaaa aaagagataa aatggtattg agaataatta
    10681 ctcgcatgat gagccaattg attaataaat gaatcatctc caatattgcc tcatgtaatt
    10741 aactacaaaa tttatatcta acaagtaccg ctaatagcag agctatgttt tattttaagt
    10801 cagaaaataa tgtaaaaata gtccaatacc ataagagaga gaggctgtta cagtgccaat
    10861 tattagtgtc tcaaaaccgg agtataacca ggatttggtt gaccagtggc ttttgattga
    10921 accgatgaca aaaaatgtaa ttgcagtgaa aaaacaagac cagatgaacg aattgttcat
    10981 gaccagcacg taaggaatta atggaataat tccaaaaagt agaaacgcac taaaagtata
    11041 aagcgcagat tttagcggga atcgaagtgt agcaggcaac ccatattctt cctgtaacat
    11101 agtttttatc cataatgctc tgttcgctgt aatttcctca acaacttgat ctaatgtttg
    11161 gccatgcaaa cctttatttt gaaaaatttg ttttatttcg tttttttcac cttctggaat
    11221 aaaatcaata tgtttttctt caattgtttt ataacgctga aattgatcga cctcagattt
    11281 ggtacccaga taattacttg ctgccattga aaaaccatca gcaagcaagt tggcaaaacc
    11341 taaaattaga ataactaaag aagataattg tccacctaca acaccggaaa caatggcaaa
    11401 agtagtgaca acaccatcaa ttcctccata tatccaatct cgcaagtaat tttgagatgg
    11461 ggcctgtgct aatcgatctc tgatttcttt ttgcgaatgt ccatgctcta aattttccat
    11521 agcagatcct ttc
//
LOCUS       DBNCWK010000101        11277 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.101, whole genome shotgun sequence.
ACCESSION   DBNCWK010000101 DBNCWK010000000
VERSION     DBNCWK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.101"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..377
                     /locus_tag="AC06IO_002183"
     CDS             <1..377
                     /locus_tag="AC06IO_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875359.1"
                     /translation="AQQDMKVKVNVLRIPANEREANIVAVYSILINKDLMGDMDHIPN
                     VIWQIKSIIENINLDDDDDIARSICLIKEKIENSNENYTNKNIMDFLNAFSKKSDLTF
                     RQIRQELAQSNSEMKKILDTYD"
     gene            complement(624..2162)
                     /locus_tag="AC06IO_002184"
     CDS             complement(624..2162)
                     /locus_tag="AC06IO_002184"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIO9875360.1"
                     /translation="MQNANAKIQNINEIMFSDRVMLLNKNLLVSIPANFLCALIIYID
                     FNKTTTDQEMLSVWFMVGVTVFALHGGLFLFNYYRPLPSKYLLKWLISVTAIYGALWG
                     IAGSVLIPQNDLLNQMIVIIIIIGIASGGLHILQPSFLASLLFFTLTLIPLCVWFFQQ
                     NTLNYWFLGTALLIYFCFLSIISWMGYGLLNKNFKLRYENLDLINKLYAINANLEESE
                     LRFRSAFNSAAIGMAIVSLEGMWLKVNQSLCQIVGYSEEELLETNFQSITYPEDLELD
                     LGYVRQLLEGDIRFYHMEKRYIHKNGSIIWILLSASLIRDAENKPLYFISQIQNIDAQ
                     KRAEQELQHIAYHDTLTGLGNRKLLELSFDQALAHAKRHQTQIAIMFMDLDYFKNVND
                     KLGHDIGDLLLVEIGKRLQTILRSTDLIVRHGGDEFIIALTELSNVNQVIEIANKILI
                     AVSKPITIKRYGISITGSMGISIYPYDGDDSDTLIRKADEALYRVKIGGKNNFQLFNT
                     ALHK"
     gene            2386..3642
                     /locus_tag="AC06IO_002185"
     CDS             2386..3642
                     /locus_tag="AC06IO_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875361.1"
                     /translation="MENKKKRVVVATAGTFLEWAEFTYYAYIANIISGLFFPKLGNHL
                     GLIATFSVFALSYFFRPLGALFFGYIGDKMGRRVALQSSIMLMGLSSLLIGCLPSYNS
                     IGILAPILLLIFRCIQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGG
                     LMSTLIYLPHMPEWAWRIPFLLGTLSCFCSMYFRKNLSESPPYLTLSKKKSSNPLKIL
                     FENYKKQLIRAVLLIAALGIYIYIMNVYYASHLAKYTTLSEAQTKFVVTLGQGLVVLF
                     IVLTGMYADRIDERHIIKTGLWGYFIAAPVAFLIPQTNSFALILISQIPYALCNALVG
                     TPIFKLLNDFFPTQIRYSGVSVAWGVSMAIFGGTAPLVANYLQYSFKLTTAPIFYILL
                     SASIALLVLHDKKSYKITSIKLRSDN"
     gene            complement(3757..6957)
                     /locus_tag="AC06IO_002186"
     CDS             complement(3757..6957)
                     /locus_tag="AC06IO_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIO9875362.1"
                     /translation="MFTRLIAWSLHNRLLILALTIILCLLGGYTLKQMPVDVFPEFAP
                     PQVVVQTQAPGMATQDVETLITYPLESAINGTPGVASVRSKTSVGLSTITVVFDDKTD
                     IYLNRQLVNERIQQVINRLPPGVSSPVMLPVTSAVGWLLKYALVSHTASPETLRTISD
                     WTIRPRLLALGGVASVVSLGGEVKQYQVQLDPKRMLAYGITVEEVRQALATGNQNVPG
                     AFVHQGGTEFVVSTIGRIKTLDDIKKTLIVVRKGVPVTVNNVATVAFGGEIKRGDASF
                     NAEHAVIGTVSKIYGADTLTTTLKVEQALADIKKSLPTGIELNTEVFRQANFIESAIH
                     NLTKALIEGAVIVIAVLFLFLMNWRASFITFLSMPVSFIVGILVLHYFGFGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRLNRLAENPLPAIEIVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGIPERIFSPLAIAYIASVLGSLVVSITMVPALCYLLLARKTEKNQDV
                     KVAVHALSEDERYHCTQEETVKQAQTETRFVIWLKKHFLTGLNWSLAHCKTVVAISIA
                     AFVFALSLLPFFGTSFLPEFHEGNFIIVMSTLPGTSLNESMRLGKQVSKTLLKYPQVI
                     SIAQRAGRSELDEDALPPNISEFDVHLNFGKDKTMSPDELLQSIRTDLANIPGALFNV
                     GQFIAHRMDEVLSGVRAQVAVKLFGDNLATLNELGESVESILKTVPGVVDINKEQQIN
                     VPQLVIKIDREKAARYGVNIGQISEDIQILLNGVTVSSILEGQRTFDLYLRMGETERD
                     SVKAIQNMLIDAHGTANNQETKIPLRAVAEIQLEPQPFAINRENVQRVLVIGFNVQGK
                     DLGSVIQEVQHQIKEKISLPGGYFVQYGGQFESQQQASRVIVLFGALAVFIMLVLLHK
                     AFGTFREALLVMFNLPLALIGGVVSLFIASGDMSVAAMIGFITLFGIAARNGIILVSH
                     YNQLRSLGQEREQVVIQGTLDRLVPVLMTAATAALALIPLLWGSPAGKELQRPLAQVL
                     LGGLLTSTVLNMLVVPTVYNAFEIWHEKRRQLKEIY"
     gene            complement(6958..7926)
                     /locus_tag="AC06IO_002187"
     CDS             complement(6958..7926)
                     /locus_tag="AC06IO_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIO9875363.1"
                     /translation="MRLQLKIRMMIMTVFFLGLLSPIQVSFAHGGNIEVSEGGTKGPV
                     HLTPEQARMLDIKVVQATHRPMAQLLGLNGQIQLLPDAQADVSIRISGSVTAIDVNLG
                     DSVKAGQRLATVQSRLVGNPPPSVAVNAPIAGIIDARNINLGQAVEPNTVLFHISNRD
                     KLLVIAQVYEEDLGKVKVGQEVNIHALSYPKQTFPGQVTLIEPNLDPMTRTVNLRITL
                     DNKEGLLKPGMFVRANVILTHNKAALTVPNAALLQADNQQFVFVQNGDTYKRVNVKVG
                     AVEDDYSEITEGLVPGDLVVTQGNRELYTLWLSGGRKLQSGQEEHH"
     gene            complement(7910..9235)
                     /locus_tag="AC06IO_002188"
     CDS             complement(7910..9235)
                     /locus_tag="AC06IO_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIO9875364.1"
                     /translation="MEYHRVRRFSYGLVLLLLYASLATAGNALTLKEVTQIALANNKD
                     LKAARYNVSIAKARLLQAGFWPNPSLNLSNNDDRLFTDEGEYSRSAGFSQAFPISGRI
                     AKQKTVARLDVLKAVAEIREAERQLSAKVASAFYTAVLTERRIQQVNYLLRINRELVD
                     VIHNRYHAAEISKLDDNAARIEYLRIVQEKHLLHSLSISQYATLNQLMGREANSSLSL
                     KMDFAAAKKLPVLDALKTQALKNRPDRQSIALSIERANADRRLAKAERFADWTVGLGI
                     QQDKIVVEEGPPQPADRTLGVTLSVPLPLLNRNQGRILEASHTGTQAMMSLRALDLSI
                     ATEIASNYEQLKALRKSLEETQRVSLQLTIENVKLARDSYENGQISFLNVLQVQKQQN
                     DLQTTYLNTLEKFLQVYVALCTAIGPGKTDGFCSYLEYQSNGDSNEIAA"
     gene            complement(9594..11216)
                     /locus_tag="AC06IO_002189"
     CDS             complement(9594..11216)
                     /locus_tag="AC06IO_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIO9875365.1"
                     /translation="MVYYINLKEQPLPQDKEILTQLIKQYLLSGSSQSALFANHPYTL
                     NDEDGNEIEFLLSHDIYKNQREDGITEFLILNGSQIGRGTKGVVIQSNHAMYLIDDEL
                     VIFPKENVLKVLYQNFINAEELIKQESVNYSILSGNSTAELVNLQGGNQGFFMQKEEG
                     YALEELLEDIDSLQKLTFSDKMELAISFLTELENLHNKGYAHGDLTFKNILYNEKNNQ
                     LKIIDAGEMTVADERLLAQDILIARDMVSLLFTTDEANQFKASSYWSDEPTIRQIVNP
                     NLVEAKLNRILNSKYSCISEFKSAIEWNYNEYLSQLNPLLQRNELSKVEREASNLQAY
                     KDQMDKQQLLGEIQGCKKPYWAMKGSLLYADQYRLKVFFDYLDTIGSAFKNDKINEKS
                     ARAAYDFLTAQINKLFEDHEKNSQLSFKSFSEDVQQFLNHVNTFLRKHPLASSNSIAS
                     IIRLLKQLDNQASFEPEQSLKTFSSYKEVMIQKLLEPFETLVAEMTSAPATVSDFIEK
                     QSDTKRRLQEMKSSANQDKQPIFTPSLMRGPS"
BASE COUNT         3538 a         2281 c         1992 g         3466 t
ORIGIN      
        1 aagcacagca agacatgaag gtaaaagtaa atgttttaag aattccagcc aatgaaagag
       61 aagctaatat agtagctgtt tattcgatat taattaacaa ggacctgatg ggagatatgg
      121 atcatatccc aaatgtcatt tggcaaataa aatcaattat tgaaaatatc aatctggatg
      181 atgatgatga tattgcaaga tcaatttgtc taattaaaga aaagatagaa aattccaatg
      241 aaaattatac caataaaaat atcatggatt ttctaaatgc atttagtaaa aaatcagatt
      301 tgactttcag acaaatccga caggaattag cacaatccaa ttctgaaatg aaaaaaattc
      361 tggataccta tgattgagat gatatgattc atagacatca tttcaattca tattatccat
      421 cgtctaaagt aagcaaaaaa gcataggcta atttttacct tgatactaaa aatacaggct
      481 ctgtttgcca tcgatatggc ttctctcgtc agacagataa ccaagaaaat ggcaatgttg
      541 atatttagag aggaaaccgg gtaataatct gacaaactaa gaacaaaggg gctggaaaga
      601 agcttgttga ttaaaaaatt attttactta tgtagtgcgg tattgaacaa ttgaaaatta
      661 ttttttcccc ctattttcac cctatataat gcttcatcag cttttctaat taatgtatct
      721 gaatcatcgc cgtcatatgg atagatactt atacccatac tgcctgtaat tgaaatacca
      781 taacgtttga ttgttatagg cttgcttaca gcaattagaa ttttatttgc tatttctata
      841 acttgattta cattggataa ttcagtaagc gcgatgatga attcatcacc accatgcctg
      901 acaattagat cagtagacct taaaatagtc tgtaatcttt ttccaatctc aactaataaa
      961 agatccccaa tatcatgacc caatttgtca ttcacatttt taaaataatc caaatccata
     1021 aacataatag caatttgcgt ttggtgacgt ttcgcatgag ctaatgcctg gtcaaatgat
     1081 aactctaata gctttctatt tcccaaacca gttaaagtgt catgatatgc gatgtgttga
     1141 agttcttgct ctgctcgttt ttgagcatca atgttttgaa tttgcgagat aaaatagagt
     1201 ggtttattct cggcatctcg gattaaagaa gcactaagta aaatccaaat gatactacca
     1261 tttttatgaa tatagcgttt ttccatatga tagaatctta tatccccctc taataattgc
     1321 ctcacatagc ccaaatcgag ctctaaatcc tctggatatg tgatcgattg aaaattagtt
     1381 tccaataatt cttcttctga gtatcctaca atctgacaaa gcgattggtt aacctttaac
     1441 cacatcccct ccaaagaaac tatagccata ccaatagcag cagaattaaa tgcggaacgg
     1501 aaacgtaact cactttcctc caaattagcg ttaattgcat acaatttatt aattaaatcc
     1561 aaattttcat aacgtaattt aaaattctta ttaagaagtc catatcccat ccaggatata
     1621 attgataaaa aacaaaagta aatcagcaat gcagttccaa ggaaccaata gttaagggta
     1681 ttttgctgga aaaaccagac gcatagagga ataagagtta atgtaaaaaa taatagactt
     1741 gctaaaaagc tgggctgaag aatatgtaat ccacctgatg cgattccaat aattataatt
     1801 atgacaatca tttgatttaa cagatcattc tggggaataa gaacagagcc tgctattccc
     1861 catagagccc cataaatcgc agtgacgctt attaaccatt ttaaaaggta tttggatggc
     1921 aagggacgat aataattaaa caaaaataaa cctccatgca gagcaaatac tgttactcca
     1981 accataaacc atactgagag catttcttga tctgttgttg ttttattgaa atcaatataa
     2041 ataattaatg cacataaaaa atttgcagga atgctcacta ataaattttt atttagtaac
     2101 attacgcgat ctgaaaacat tatttcattt atattttgaa tctttgcatt ggcattttgc
     2161 attcagcgct cactgagata aagtgacaaa ttcaaaacct caatttgaac cctattgtat
     2221 taatatattg tattaatata gtacaaaaat acttaattgt tagaagcaga atgtgacact
     2281 atagtaactc tatgaatctg ttatatcatt cagttacata gaataatgag agcaatttaa
     2341 ggtaaaatct ctctacatca ctttaatatt ctattgattt attgtatgga aaataagaag
     2401 aaaagggtcg ttgttgcaac cgccggaact tttctggaat gggctgagtt tacttattac
     2461 gcctatatcg caaatattat ctcgggattg ttttttccaa aattgggaaa tcatcttggt
     2521 ttgatagcaa ccttttctgt tttcgctctc agttattttt ttcgaccatt aggagccctc
     2581 ttttttggat atattggaga caagatgggg cgtcgagttg ccttgcaatc ttctattatg
     2641 ctgatgggcc tatcttctct gttgattggt tgtcttcctt cttacaacag tatcggtatc
     2701 ttggctccaa ttctcttgct catctttcgt tgcatacaag gctttgccgt atcaggtgaa
     2761 tttaatggca gcgcgatcta tctgattgaa catgattcac aaaaaccatg ccaggctggt
     2821 agctggactg gatttgcttc cgcattgggt atgatgtttg gcggattgat gtctacttta
     2881 atttatctac ctcacatgcc tgagtgggca tggcgtatcc ctttcctgtt gggaacttta
     2941 tcctgctttt gttcgatgta ttttcgtaaa aatctgagcg aatcaccgcc ctacctcaca
     3001 ctaagtaaaa aaaaatcatc caatccactg aaaattttat ttgaaaatta taaaaaacaa
     3061 ttaattaggg cagtattatt aatagctgca ttaggcattt atatctacat tatgaatgtt
     3121 tattatgctt ctcatttggc aaaatacact acgttgtcag aagcccaaac caaattcgtt
     3181 gttaccttag gacaaggatt ggttgtacta ttcattgttt taacaggaat gtatgctgat
     3241 cgaattgacg aaagacatat tataaaaacc ggtttgtggg ggtattttat tgcggctcct
     3301 gttgctttcc tgatccctca aaccaattct tttgcattaa ttctcatatc gcagattccc
     3361 tatgcattat gcaatgctct ggttggcaca ccaattttta aactattgaa tgattttttt
     3421 cctactcaga ttcgatatag cggtgtttct gttgcatggg gagtaagcat ggctattttt
     3481 ggaggaacag cgccattggt tgccaattat ttacaatatt cattcaaatt aacaactgcg
     3541 ccaatattct atatactttt atctgccagt attgccttgc tcgttttgca tgataaaaag
     3601 agttacaaga taacctccat aaaattaaga tcagataatt gaataattga ataaacgact
     3661 tatcaccatt gcaaaaacat gtctacgcaa gcatctaatc aatttgtaac aattgcttct
     3721 taatagcccc cattgtcaga gtcatcatga ctctgattaa taaatttctt tcaattgtct
     3781 tctcttttcg tgccaaattt caaaagcatt ataaactgta ggtactacaa gcatatttaa
     3841 aaccgtagaa gttaataatc cccctaatag cacctgcgcc aaagggcgct ggagttcttt
     3901 accagctggg gaaccccaca ataggggtat taaagccaga gccgctgtcg ccgctgtcat
     3961 taatacaggg accaggcgat caagggttcc ctggataact acttgttcac gttcctgccc
     4021 cagagagcgc aattgattat agtggctcac taaaataatc ccattacgtg ctgcaatccc
     4081 aaacagagta ataaaaccaa tcatggcagc gacactcatg tcaccgctgg caatgaataa
     4141 agaaacgact ccaccaatca atgccaaagg taagttgaac ataaccaata gagcttccct
     4201 aaatgtgccg aaagccttat gaagaagtac caacataata aaaactgcca gcgcaccaaa
     4261 aagaacaatc acacgagatg cttgttgttg actttcaaac tggccgccat attgaacgaa
     4321 ataaccacca ggtaacgaaa tcttttcttt aatctgatgt tgtacttctt gaatcacact
     4381 acccaaatct ttcccttgca cattaaaacc gataaccaaa acacgttgca cgttttctcg
     4441 attaattgca aacggttgag gctctaactg aatctcagcc accgcacgca aagggatttt
     4501 tgtttcttga ttattggccg taccatgggc atcaatcagc atattttgaa tggctttaac
     4561 actatcccgc tctgtttcac ccatacgcaa atacaaatca aacgtcctct ggccttcaag
     4621 aatactggat accgtcacac cattaagaag gatttgaatg tcctcggaaa tttgaccaat
     4681 attgacacca tagcgagccg ctttctccct atcaatttta atgaccagct gaggtacatt
     4741 gatttgctgc tctttattaa tatctactac tcctgggacg gtttttaaaa tggattctac
     4801 cgattcaccc aattcattaa gggtagcaag attatcacca aagagtttca cggctacttg
     4861 cgctctgact cctgataaca cttcatccat gcgatgggca ataaactgcc ccacattaaa
     4921 taacgctcct ggaatattcg ctaaatcagt tcgaatagat tgtaacaatt catcaggaga
     4981 catggtttta tctttaccaa aattcagatg tacatcgaac tcactaatat tcggtggtaa
     5041 agcatcttca tccaattcac tgcgccctgc tctttgggca atagaaatca cttgcggata
     5101 tttcaacaaa gtcttactga cttgcttccc caagcgcatg gattcattca atgaagttcc
     5161 cggaagagta ctcatcacaa taataaagtt gccttcatga aattcaggca aaaaagaagt
     5221 tccaaaaaaa ggaagtaaac ttaaagcaaa aacaaaggct gcgattgaga tggctacgac
     5281 tgttttacaa tgggctagtg accaatttaa accagttaaa aaatgctttt taagccaaat
     5341 cacaaagcgc gtttccgttt gagcctgctt aaccgtttcc tcttgcgtgc aatgataacg
     5401 ctcgtcttca gataaggcat gtacagccac tttaacatcc tgattctttt cagtttttcg
     5461 cgctaaaagg agataacata aggctggtac catagtaata gaaacgacaa gagaacctaa
     5521 tacggaggca atataagcaa tagctaatgg gctaaaaatc cgctcgggaa ttcctgacaa
     5581 aaagaagatg ggtaaaaata ccaggctaat gataatggtc gcataaacca ctgaactgcg
     5641 aatttccaaa accgcatcaa aaacaatttc aatcgcaggc aaggggttct ctgccaaacg
     5701 attaagtcgt aaacgatgta ccacattttc tacggtaata atcccatcat ccaccacctc
     5761 accaatagca atcgccattc ctcccaaagt catggagtta ataccaaaac cgaaataatg
     5821 aagaaccaag atccctacga taaaagaaac gggcatagag aggaaggtaa taaacgaggc
     5881 tcgccaattc atcaaaaaaa gaaacaatac agcaatcaca ataacagcac cctcaataag
     5941 ggctttggtt aaattatgaa tcgcagattc aatgaaatta gcttggcgaa atacttccgt
     6001 atttaattca atacctgtcg gcaaggattt ttttatatca gctaaagctt gctccacttt
     6061 aagtgttgtg gttaatgtgt cagcgccata aattttggaa acagtaccaa tcacagcatg
     6121 ctccgcattg aaagaagcat caccgcgttt gatttcaccg ccaaaagcga ctgtggctac
     6181 attattaaca gtaacaggaa ctcccttgcg gactacaatc agtgtttttt ttatatcgtc
     6241 caacgtctta atacgaccaa tggtacttac gacaaactcc gtacccccct gatgtacgaa
     6301 agcaccaggt acattttgat ttcctgttgc caacgcttga cgaacttctt cgactgtgat
     6361 gccataagcc agcatacgct tgggatcaag ctgtacttga tattgcttta cttctccgcc
     6421 cagtgaaacc actgaagcaa ctccgcctaa agccaataga cgtggacgaa tagtccaatc
     6481 agaaatagtg cgcaaggttt cagggcttgc tgtgtgactc accaaagcat atttaagcaa
     6541 ccaccctact gccgatgtga ctggcagcat cacaggtgag gaaacacctg gagggagacg
     6601 gttgattacc tgttgaatgc gctcattaac cagttgacga ttgagataga tatctgtttt
     6661 atcatcaaat accacagtaa ttgttgataa cccgactgag gtttttgagc gaacactggc
     6721 tacccctgga gtcccattaa tggcactttc caaaggataa gtgattaatg tttccacatc
     6781 ttgggttgcc atgcctggcg cttgggtttg aactacaact tgaggagggg caaattcagg
     6841 aaaaacatct accggcattt gctttagagt ataaccaccc agcaaacaaa gaataatcgt
     6901 taatgccaaa atcaagagac gattatgcaa agaccaggca ataaggcgag taaacattta
     6961 atgatgctcc tcttgccctg attgcagttt gcggcctcca ctcaaccaaa gtgtataaag
     7021 ctcgcgatta ccctgagtga caacaagatc accaggaact aaaccttcag taatttctga
     7081 ataatcatct tctacagcgc ctactttaac attcactcgt ttgtaggtgt ctccattttg
     7141 cacaaaaaca aattgctggt tatctgcttg taaaagagca gcatttggca cagtgagtgc
     7201 tgccttattg tgagttaaaa taacattggc ccgcacaaac attccgggtt tcagtagccc
     7261 ctccttgttg tccaaagtaa ttcgtagatt gactgtacgg gtcattggat caagattggg
     7321 ttcaattaaa gtgacttggc ctggaaatgt ctgcttggga taacttaaag cgtgaatatt
     7381 cacttcttgc cctactttca ctttacccaa atcttcttca tacacttgag ctatgactaa
     7441 taatttatcg cgattactga tatgaaaaag aacggtattt ggctcgacag cttgccctaa
     7501 attgatgttc ctggcatcga tgatgcctgc aattggggca ttgacagcaa cgctcggtgg
     7561 aggatttccc accaatcgtg attgcactgt agccaagcgc tgccctgcct tgacactgtc
     7621 ccccaaattc acatcaatcg ctgtcacact gccactaatc cggatgctga catcagcttg
     7681 agcatcaggt aataattgaa tttgaccatt taaacccagc aattgtgcca taggacgatg
     7741 ggttgcctgg acaactttga tatcaagcat cctggcttgt tccggagtca aatgaaccgg
     7801 ccctttggtg cccccttcgc ttacttctat gtttcctccg tgtgcaaaac tcacttggat
     7861 gggggaaagt aagccaagaa aaaaaacagt catgatcatc attctaattt taagctgcaa
     7921 tctcattgct atcaccgttg ctttgatact ctaaataaga gcaaaaccca tctgtttttc
     7981 cagggccaat agctgtacac aaggccacat agacttgtaa aaacttttct aatgtattga
     8041 gataagtggt ttgcaaatcg ttttgttgtt tttgcacttg tagcacattt aaaaaagaaa
     8101 tttgcccatt ttcataagaa tcacgagcca attttacatt ttcaatagtt aattgaagag
     8161 aaacacgttg tgtttcctca agactcttac gtaatgcttt gagttgctca taattactgg
     8221 ctatctccgt tgcaatactt aaatcgaggg cacgtaatga catcatcgcc tgagtacctg
     8281 tatgacttgc ttctaaaatt cgcccttgat ttcgatttaa aagagggaga ggaacagaca
     8341 gtgtaacacc caaagtacga tcggctggtt gaggggggcc ttcttccacc acaattttat
     8401 cctgctgaat accaaggcct actgtccaat ctgcaaagcg ctcggctttt gccaaacggc
     8461 gatcagcatt ggcacgctca atcgataacg caatagattg ccgatcaggg cgatttttaa
     8521 gcgcctgtgt ttttagagca tctaataccg ggagtttttt tgcagcggca aaatccattt
     8581 taagtgataa agaagagttg gcttcacgtc ccattaattg atttagtgtc gcatattgac
     8641 taatactaag gctgtgcaaa agatgttttt cttgaacaat acgcaaatac tcaatgcgcg
     8701 ctgcattgtc atccaatttt gaaatctctg cagcatgata acgattatgg ataacatcaa
     8761 ccaattcccg gttaatccgt aataagtaat tgacttgctg tatgcgacgc tctgtaagca
     8821 ctgctgtata aaaggcacta gccactttgg cactgagttg acgctcagct tccctgattt
     8881 ctgcaacagc tttcaacaca tccagacgag ccactgtctt ttgttttgca atacgccctg
     8941 atatgggaaa tgcctggcta aagccagcac tgcgcgaata ctctccttca tcagtgaata
     9001 aacgatcgtc attatttgaa agattcaagc ttggattagg ccaaaaacct gcttgtagca
     9061 accgggcttt tgcgatagat acattgtaac gggcggcttt taaatccttg ttgtttgcta
     9121 aagcaatttg ggttacttct ttaagagtca aagcgttccc cgccgttgcc agagaggcat
     9181 acagcagtag caggactaat ccataagaaa accgcctaac acgatgatat tccataaacg
     9241 atagctcaaa aaatctatct atcttcagtc aacatggaat cgaaaaaata atctgtggca
     9301 ggtgcaaaga tcaagagtaa tggactgtgt tttattcgaa agtaagtcat caattaatac
     9361 cttcttttta ttattgtatg atttattcag gacttacgaa aaaaaccaag gtcgaggcag
     9421 aaaatgtttt taatgaggga gtttagataa actaaatgac cgaattaaaa acatttttta
     9481 acatagagat tgggattttt cgtaagtctt gttagtatct cccagcaaat gtaattacac
     9541 aagagcagta aaataataga cgacaggctg ctctaacact tcagtgacgt tttttagctt
     9601 gggcctcgca tcaatgaagg tgtaaatata ggttgcttat cttgatttgc agaagatttc
     9661 atttcctgaa gtcttctttt ggtgtcagat tgtttttcaa tgaaatcaga tactgtagca
     9721 ggtgcactag tcatttcagc cactagtgtt tcaaatggtt ctaataattt ttgaatcata
     9781 acttctttat acgaagagaa agtcttaagc gattgttccg gctcaaaact cgcttgatta
     9841 tccaactgtt tcaatagccg aatgatggaa gctatcgaat tagaggaggc cagcggatgt
     9901 tttcttaaaa aggtattcac atgatttaag aattgctgca catcttctga gaaacttttg
     9961 aagcttaatt gtgagttttt ctcatgatcc tcaaacaatt tattgatttg tgctgttaga
    10021 aaatcatagg ccgctctggc cgacttttca ttgattttat catttttaaa tgctgaacca
    10081 attgtatcca agtaatcaaa aaatactttt agacgatatt gatccgcata aagcaaactg
    10141 cctttcatag cccaataagg ttttttacac ccctgaattt ctcccaggag ttgttgttta
    10201 tccatttgat ctttgtaagc ctgtaaattc gaagcttctc tttccacttt tgaaagctca
    10261 tttctttgca atagaggatt caattgactt agatattcat tgtaattcca ttcaatggcc
    10321 gacttgaact ctgaaataca actgtactta ctgttcaaaa ttctatttaa tttggcttcg
    10381 actagatttg ggttaactat ttgcctgatt gtaggctcat ctgaccaata gctcgatgct
    10441 ttaaattgat ttgcttcatc cgttgtaaat aagagagata ccatgtccct ggcgattaaa
    10501 atatcctgtg ctaaaaggcg ttcatctgct actgtcatct ctcctgcatc aatgattttt
    10561 aattgattat ttttttcgtt atataaaata tttttgaaag ttaaatcgcc atgggcgtag
    10621 cctttgttat gcagattttc caactcagtt aaaaatgaaa tggctaactc cattttgtct
    10681 gaaaatgtca atttttggag cgagtctata tcctcaagaa gttcttccaa ggcatatcct
    10741 tcttcttttt gcataaaaaa tccttggttt cctccttgca aattgactaa ttctgcagtt
    10801 gaatttcctg ataaaatcga ataattgaca gattcctgtt ttattagctc ctcggcattg
    10861 ataaagttct gatataaaac tttcagtaca ttctctttag gaaaaattac caattcatca
    10921 tcgattaagt acatagcatg attactctgt atcactacgc ctttggttcc tctccctatc
    10981 tgtgagccat ttaaaattaa aaactccgta ataccatcct cacgttgatt tttgtaaata
    11041 tcatgactta agaggaattc tatttcattt ccatcttcat cgtttagcgt ataaggatgg
    11101 ttggcaaata atgcggactg actggaacca gacaataaat attgcttaat taactgcgtt
    11161 aaaatttctt tatcttgagg taaaggttgt tccttcaaat ttatgtaata aaccataatg
    11221 ctctcctaca aaccaattgg ctacatatat ttgagcatat tgataaatat taataca
//
LOCUS       DBNCWK010000102        11255 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.102, whole genome shotgun sequence.
ACCESSION   DBNCWK010000102 DBNCWK010000000
VERSION     DBNCWK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.102"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            66..476
                     /locus_tag="AC06IO_002190"
     CDS             66..476
                     /locus_tag="AC06IO_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor family protein"
                     /protein_id="HIO9875366.1"
                     /translation="MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTAS
                     TVYLKHLPTGMEVKCQESRSREDNRYFARQRLCEKLQAVFSDEKTKTQQRIEKIKRQK
                     RRRSRRSKQKMLDEKSRQSQLKTLRKNPRSHDIE"
     gene            743..1627
                     /locus_tag="AC06IO_002191"
     CDS             743..1627
                     /locus_tag="AC06IO_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HIO9875367.1"
                     /translation="MAGTSLLTLIDDIATALDDIAAMTKVATKKTAGVLGDDLALNAE
                     QIIGIHANRELPVIGAVAKGSAVNKLILIPLAILINFFVPALVGILLILGGLYLCFEG
                     FEKIVEKIFHKKNEPAKKKIDTQTIDMRQFEKEKISGAVRTDFVLSAEIIVITLGTVA
                     ATSLINQIAVLTLISLIMTVGVYGLVACIVKLDDLGFILREKKHPLKQIGILLIGAAP
                     ILMKILSVAGTIAMFLVGGGIITHQITAIHHFTENLSAMMSIGFDFFIGIISGAITAA
                     LYFTFKKMKSKFKLMNTK"
     gene            1782..2237
                     /locus_tag="AC06IO_002192"
     CDS             1782..2237
                     /locus_tag="AC06IO_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIO9875368.1"
                     /translation="MPDIKHYLKPIILFSGLLVAPFAMAEATKAEETSTVKLSTILQQ
                     LSDQGYKNIHDAEIDDGVLTVEGSNEQGVKFEIHIDPATGKILKKQEHPQPKYSIIDI
                     TKKIENEGKFKIIEIEFDEGIYKIKGETANHEKRKLYIDPNTGETKTDH"
     gene            complement(2319..3092)
                     /locus_tag="AC06IO_002193"
     CDS             complement(2319..3092)
                     /locus_tag="AC06IO_002193"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIO9875369.1"
                     /translation="MYKTVHFYSNDLKLEGKLEEPTGKCLGYVLFAHCFTCGKDIAAA
                     SRIASALVSNGFAVLRFDFTGLGSSEGSFAETNFSSNVEDLVAAADYLRTYYRAPVLL
                     IGHSLGGAAVLLAAKKISEVKAIATIGAPASAHHVKHHFSADLSKIESDGEAQVTLGP
                     RSFTIKKQFLEDIDRYQETITSDAGKALLIMHSPIDKVVSIKEAEKIYKAAQHPKSFI
                     SLDKADHLLSNKRDSQYAAEVIAAWASRYLTPSSEDQSG"
     gene            complement(3592..4308)
                     /locus_tag="AC06IO_002194"
     CDS             complement(3592..4308)
                     /locus_tag="AC06IO_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIO9875370.1"
                     /translation="MTGKIKIAVLGLGISLGCFAGEMGAIQKSGFYLQAMLDYNWFHY
                     NHAYTASFFGPQNISALDASIDNQWGYGFGLGYRFNDYVRTAITVQARPDVNFAVTDD
                     APETATGHFDNYTYMLNGYLSSPNFSVMNFNPYIMAGVGVAHNKTTNIYWPAAAQTEF
                     ADKATKFAWQFGIGSLYALSDSLLIDINYNFISIGEVRNSGQYNAIAANNTPASGAPT
                     KFTNVYSNQAEIGIHYQFNT"
     gene            4839..5258
                     /locus_tag="AC06IO_002195"
     CDS             4839..5258
                     /locus_tag="AC06IO_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIO9875371.1"
                     /translation="MKTYRKELWFNIPERMGFINITDKVIECLKESRIQEGLVLVNAM
                     HITASVFINDDESGLHQDYKKWLEQVAPHEPIRQYRHNDTGEDNADAHIKRQIMGREV
                     VVAITEGRLDFGPWEQIFYGEFDGRRDKRVLVKIIGQ"
     gene            complement(5396..6133)
                     /locus_tag="AC06IO_002196"
     CDS             complement(5396..6133)
                     /locus_tag="AC06IO_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIO9875372.1"
                     /translation="MNNSDGQSMTRIDEMVPDIYRISTSISQEVIPGGFSFNQYLIVD
                     KKPLLFHTGPLQLFPAISQAVAKVIPVKSLSFIAFSHFEADECGSLNEWLKVASDAVP
                     LCGQIAAMLSINDIAIRSPKIMSDLEQVSLGKHNVQWLDAPHLPHGWDCGFLFETTSK
                     TLFCGDLFTQPGADHKPVTEEDILESSEIMRQTMDYYAHSKNQRQLFEKLASTHPKIL
                     ACMHGSAWKGNGFQLINALADRVCGNI"
     gene            complement(6227..7204)
                     /gene="ravD"
                     /locus_tag="AC06IO_002197"
     CDS             complement(6227..7204)
                     /gene="ravD"
                     /locus_tag="AC06IO_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase RavD"
                     /protein_id="HIO9875373.1"
                     /translation="MNLKKEVFLNQNCAEMMIKKAARLILGSDLDFAYTRDVQDIQVD
                     LGPAFMFSPDEEKTLWVSGKNQETLEKDLATLNKSSVYFFRTGTQGGAGHWQVLYYEA
                     AKSGWVSYSSQSNHFQVTDSNGKLTASGKGLLVPHANWGKENGNYAFLLVNASAQNII
                     HAANFVYILRTQNEVAAIEYCALNHKFHPEIKRTARAKAEPETRTKGEIHNLPKDLSC
                     ENLKTIYSKHVALLNVINRLEAGSKSYNPYWMGSQAKLNTILGALGACYQKGLDVDSE
                     LSNSNSELSKAINIRRLPSLFSFWGGKNESPVESRKIIDENRIGRLNNL"
     gene            7515..7994
                     /locus_tag="AC06IO_002198"
     CDS             7515..7994
                     /locus_tag="AC06IO_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875374.1"
                     /translation="MKEKTAKQLKLFIIYLISYWLLSIISCLIAFGYDDSLRMLLASP
                     KSDLSGTLLFLSSFIAAALLFVFRYKTFSDKPYPYFIFGFYVGNVSLLMLFILDAFIR
                     ELIVWKFPEFLLVFISPFVELVLSYLFFGFAFLAIIPAVTSAIILYAVQRKLLLPPI"
     gene            8169..8381
                     /locus_tag="AC06IO_002199"
     CDS             8169..8381
                     /locus_tag="AC06IO_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HIO9875375.1"
                     /translation="MGIVKISDELHEATKLMARAMSRSINSQAEYWIRIGKLAEENPS
                     ITYTDILKILVHQASLGEIYDVSEDA"
     gene            8362..9123
                     /gene="map"
                     /locus_tag="AC06IO_002200"
     CDS             8362..9123
                     /gene="map"
                     /locus_tag="AC06IO_002200"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIO9875376.1"
                     /translation="MLVKTPEAIEKMRVAGQLAASVLEMIEPYVIAGTSTKELEQICR
                     QYIVEDLKAIPSTLNHYGFPACICTSINHVVCHGIPSEKKLKNGDIINIDVTVQKDGY
                     IGDTSKMFLVGNIKPFAKKLVEVTQECLYKAISIVRPGAHLGDIGNIIQTHAEQHGYS
                     IVREFGGHGIGKSMWEEPHIMHFGKPNTGLRLQAGMTFTIEPMLNLGRKEVKTLGDGW
                     TVVTKDHKLSAQWEHTILVTDNGHEILTLRADEHL"
     gene            9228..10316
                     /locus_tag="AC06IO_002201"
     CDS             9228..10316
                     /locus_tag="AC06IO_002201"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIO9875377.1"
                     /translation="MPCEKCDSVTTYHLSGTYQLLILPPRGHCYSKLLKHLTENNFDI
                     NKRDSGIIELLFTAERAQDIGHALERCYSQAELEDSKAVLLPIDIADRNVERVLNHTH
                     SLKKIVGLCLSQWLVGLIENQGLSTFCQPILHNTTLKPYGFECLLRGKQDNNILPSSD
                     LFGAAKSSDMLFLLDKKARICHIENMSKIDIAEKKIFINFNPTAIYDPLFCLTTTNQS
                     LKKTSIKPAQIVFEVTESDEVKDKKHLLKIIDYYRNQGYCVALDDLGSGYSSLNLLTE
                     LQPDYVKLDQELISQIHTNEFKQIILEKICEMANKLKIKIICEGIETEDELQIIKNYA
                     IDFYQGFLFEKPMPYEKIPDYIKASTQK"
     gene            10340..11218
                     /locus_tag="AC06IO_002202"
     CDS             10340..11218
                     /locus_tag="AC06IO_002202"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIO9875378.1"
                     /translation="MLTNIERAKLQLIDETSDMVIITKAEPLDAPNGPEVVWVNKAFE
                     KISKYKANEIIGQTPRILQGKETDQNTLKMIKEAIKAQMNINVDLLNYTKDGTPYWIN
                     FSIVYLRDGQGNLCYLGAIEKDISAIKNLNILLSEKAKTDPLTNVYNQENFFIAGNEA
                     VKNFHQNQETVGLLFFDVDDFKGINDTQGHIYGDKILKKVAQESLKLVRKTDNVFRYG
                     GDEFAIIFSGIDVKILERKAQQLQKKLAKSHISVSIGGTISKQSDKSINTLLARADEA
                     LYYVKANKKGLFFIRK"
BASE COUNT         3655 a         2187 c         2095 g         3318 t
ORIGIN      
        1 tttgattgaa ttcagctatt ataatgcttt aaaggcatta ttttatggga taataaagag
       61 acttcatgat tggcaaagat aaatgggaaa tcctgactga gaagatgacc aatcttcata
      121 tctatgaaac agatctcagc gaaaaattta tactcggcag tggtaaaggc ggacaaaaac
      181 tacataaaac ggcatcaaca gtctatttga aacatttgcc taccggtatg gaagtaaaat
      241 gccaggaatc caggagtcgt gaagataacc gctattttgc gaggcaacgg ctttgtgaga
      301 aattacaagc cgtattcagc gatgaaaaaa caaagacaca acaaagaatt gaaaagataa
      361 aacgccagaa gagacgacgc tcgagaagat ccaaacaaaa aatgttggat gaaaaatcaa
      421 ggcagagtca acttaagaca ttaagaaaaa acccacgatc tcatgacatt gaataatagg
      481 cacattagcc acttatcatt ttagttaaat caaaataccc ggtaacgtcc ctaaagtttt
      541 ttgtagactt ctgaacttta accaaggggc attattgtca gtttgctttg atcaattcct
      601 tctcccacgt ctggtgattt tgaaattgaa attttcatat cgtcaggttc gagtctttga
      661 aaaggagtta agacatcttt ttgcaatgct ttgaatttgt tttagtattg aaaggaatca
      721 ttaaatcaag gtcagatcca atatggcagg aacaagttta ctaacattga ttgacgatat
      781 tgcaacggca ctggatgaca tagctgccat gactaaagtg gcaactaaaa aaactgccgg
      841 tgtgttgggc gatgacttgg cactcaacgc cgagcaaata ataggcatac atgcgaacag
      901 agaattacct gtcataggag ctgtagccaa aggctctgct gttaataaat taatattaat
      961 tccattagcg attttaataa attttttcgt ccccgcactg gtgggtatcc tattgatttt
     1021 gggcggattg tatttgtgtt ttgaaggttt tgaaaaaatt gtagaaaaaa ttttccataa
     1081 aaaaaatgag cctgcaaaaa agaaaataga tacacagacc atcgatatgc gccaatttga
     1141 aaaagaaaaa attagcggtg ctgtacggac ggattttgta ttatcagctg aaatcattgt
     1201 aatcacctta ggcacggtcg cggcgacatc tctgattaac caaatagcgg tattaacttt
     1261 gatttcatta attatgactg ttggcgttta tggtttggtt gcgtgcattg tgaaattgga
     1321 tgatctgggc ttcattttac gggaaaaaaa gcacccgctt aaacaaattg gaattctttt
     1381 gattggagca gccccaatac ttatgaaaat tttaagtgtg gcaggaacaa ttgccatgtt
     1441 tttagtagga ggagggatta ttactcatca aataactgcc attcatcatt tcacagaaaa
     1501 cctgtctgca atgatgtcta taggatttga tttttttata ggaattattt caggtgcaat
     1561 tacagctgct ctatatttta cattcaagaa gatgaaatca aaatttaaac taatgaatac
     1621 gaagtaagta ctgtaagagt cgaaatccat tgtaaaatca agtaactttg catttcattg
     1681 ctcaaatgac tatagttaaa caatgatttt ttaacacaaa acctaatcaa gcgatttttt
     1741 ttgcgatttt aataccacac taccaatagg agataagcag gatgccagat atcaaacatt
     1801 accttaaacc gatcatttta ttttcaggac tattggttgc tccttttgcc atggccgaag
     1861 caacaaaagc tgaggaaact tctactgtaa aattaagtac tattcttcaa cagttgtctg
     1921 accaggggta taaaaacatt catgatgctg aaattgacga tggcgtattg actgtcgaag
     1981 gcagcaatga acaaggtgtt aagtttgaaa ttcacatcga tccagctact ggcaagattt
     2041 taaaaaagca agaacatcca caaccaaaat attccatcat agacattacc aaaaaaattg
     2101 aaaatgaggg taaattcaaa atcattgaaa ttgagtttga tgaaggtatt tataaaataa
     2161 aaggtgaaac agccaaccat gaaaaaagaa aactgtatat tgaccctaat accggggaaa
     2221 ccaaaacaga tcattaactc cacatgaaag agcaagtctt tcctttccct tagtacaacc
     2281 aattatttcc ctaaggaaaa gggagaggct tgccctactc aaccactttg atcttctgat
     2341 gaaggagtta aataacggct ggcccaggca gcaataactt ctgcagcata ttgtgagtcc
     2401 cttttgttac ttaacaaatg atcagcctta tcaaggctga taaaactttt ggggtgttgg
     2461 gcggctttat agattttttc ggcctcttta atggatacta ctttatctat gggagagtgc
     2521 ataatgagta aggctttacc agcgtcgctg gtaatcgttt cctggtatct gtcaatatct
     2581 tctagaaatt gttttttgat agtaaacgag cgtggaccaa gcgtaacttg agcttcacca
     2641 tcagactcta ttttacttaa atcagcagaa aaatggtgct taacgtggtg agcactggca
     2701 ggcgcaccaa tagtagcgat tgcctttact tcagaaattt tcttggctgc aagcagtaca
     2761 gcggcacctc caagactatg acctatgagc agaacaggag ctcgataata ggttcttaaa
     2821 tagtcagcag cagcaactaa atcttcaaca tttgaagaaa aattcgtttc cgcaaaggaa
     2881 ccttcactac tgcccaagcc tgtaaaatca aagcgtaaca ccgcaaatcc atttgagacc
     2941 aaagcgctgg caattctgct tgcagcagct atatctttac cgcaggtaaa gcaatgagca
     3001 aataaaacat aaccaagaca tttccctgtc ggttcttcca gtttaccttc cagctttaaa
     3061 tcgttactgt aaaaatgaac ggttttgtac attgcatatt ccttatttat cttgattaga
     3121 tgctcataca gaacattcaa atctgtttat caatcctttt tcttgctctg acattagttt
     3181 gattgattac tgatccagag tttggatagt ttcacagtgt tcacatacta acaaatatag
     3241 tcatatttaa cccaatttcc caatctcttt cgactcttta ttcactttgt gtcatggtgt
     3301 tgtgaagcgt aatcaaagca acgacttatc tgcactacac tccacttaaa gcactgcttg
     3361 tttaacatca tggaagattg actgcccact ttttccatca tgaggtaaag tccgtaaaat
     3421 tacgggaaaa tttgccagga aaactatgag tgaagtacca ggcagctaaa aaccagacaa
     3481 gacctgagta cgtaggtagt tttcatttca gccgagtaga tagcaatagg caggttaaac
     3541 agtggctccc attgatgtca tctttttact tcaacctgcc ttgaattggc gctaagtatt
     3601 aaactggtaa tgaataccaa tttcagcctg gttgctatac acgttggtga attttgttgg
     3661 cgcacctgaa gctggagtat tattggctgc aatagcatta tactgacctg aattcctcac
     3721 ttcgccaatt gaaataaaat tataattgat atcgattaac aacgagtcac tgagggcata
     3781 gaggcttcca ataccaaact gccaggcaaa ttttgtagct ttgtcagcga attcagtctg
     3841 agcagctgct ggccagtaaa tgttcgttgt tttattatgg gcaaccccca caccagccat
     3901 aatataagga ttgaaattca taacggaaaa attagggcta gataaatagc cattgagcat
     3961 ataagtgtag ttatcaaaat gtcccgttgc tgtttcagga gcatcatcgg tgacagcaaa
     4021 gttaacatcc ggtcgtgcct gcacagttat cgccgtccgc acataatcat taaaacgata
     4081 ccccaggcca aaaccatatc cccattgatt atcgatagac gcatcaagtg ccgaaatgtt
     4141 ttgtggtcca aaaaaggaag cggtataagc atggttataa tgaaaccaat tgtagtccag
     4201 catcgcttgc aggtaaaaac cacttttttg aatagcaccc atctcccctg cgaagcaccc
     4261 caggcttatt cccaaaccta aaacagcaat ttttatttta ccagtcattc ctctttcctt
     4321 tcttgaaaca acaaaatcaa tttgcgcgat ttttcacaaa gttatgccat tcccttgctg
     4381 atgggaatcc atactggaat gatacaagat taatccactg gatacctgaa tagcagagat
     4441 gacccgtttc gataaataca cttgaatttg ccaagtgatc cttaaattga atatatttga
     4501 aaagctatca aaggacaatc aatgttttga ttcagtgcag aaagctcgaa ccctgattta
     4561 caagctgtcc tctgtctgta tatggacttt acctgatctt gtactatctg ggtgttacaa
     4621 atcaccagta tctataattg attcggacac attaattttc caggtattcc catctttatc
     4681 aaaagcccaa aatgactcaa tgggtggttc tctctgtatc cctatgattc acgccttaag
     4741 accctgaggt ttccagaaat tttcatccat ttgaaatata accaggaatg ctatatgttt
     4801 gccggatatt gatttattgc ccaatcatta tcaaaatcat gaaaacatac cgcaaagaac
     4861 tatggtttaa tatcccagaa cgtatgggat ttatcaatat cacagataaa gtcattgaat
     4921 gcttaaagga aagtagaatt caagagggtt tagtattagt taatgccatg catatcaccg
     4981 cttccgtatt catcaatgat gatgaaagcg gcttgcatca agattataaa aagtggcttg
     5041 aacaagtagc acctcacgaa cctattaggc aataccgtca taatgatacc ggcgaggata
     5101 atgctgatgc ccatatcaaa cgacagatca tgggacgaga agtggtcgtc gctattacag
     5161 aaggaagact ggattttggc ccatgggagc aaatattcta tggtgaattt gacggtcgcc
     5221 gtgataaaag agtccttgta aaaattatcg gtcaataaat caggcctcga aggcatcatg
     5281 ggtaattgaa ggataaaaaa tcgggatttt ccttagctaa caatgccctt attcttacca
     5341 tagaaaacag aaaccccgga catgatctgc tacttaacgg aagtcgcctg ctccattaga
     5401 tgttaccaca gactctatca gccaaggcat taatcagctg aaacccattt cctttccagg
     5461 cgctcccatg catacaggcc aatattttgg ggtgtgtgct tgctaatttt tcaaataact
     5521 ggcgctgatt cttactgtgc gcgtagtaat ccattgtttg tcgcattatt tcactgcttt
     5581 cgaggatatc ctcttctgtt acaggcttat gatctgcccc aggctgggta aataaatcgc
     5641 cgcaaaatag tgttttgcta gtggtttcaa acaaaaatcc acaatcccat ccatgcggta
     5701 aatgcggcgc atccaaccac tgtacgttat gtttgcccaa agaaacttgt tccagatcac
     5761 tcatgatttt agggctacga atcgctatat cattaattga aagcatcgca gcgatttgtc
     5821 cacacagagg aacagcatca gaagctactt tcaaccattc atttagcgaa ccacattcat
     5881 ctgcctcaaa atgtgaaaaa gctataaatg ataaagactt tactggaatt accttcgcta
     5941 cagcctggct gatggcagga aaaagttgta aagggcctgt atgaaatagc aaaggcttct
     6001 tatcaacgat taaatattga ttaaacgaaa atccaccagg aattacctct tgcgatattg
     6061 aagtactgat tcgatagatg tcaggtacca tttcatcaat tcttgtcatt gattgtccat
     6121 ctgagttatt catccgttca cctttatgat taaatgaccg taaactggtc tgtccttttg
     6181 cactatcaac aacattagtg gccatgtcat gaactatttg cagctttcac aaattattta
     6241 gcctgccaat cctgttttca tcaattatct ttcttgactc tactggggat tcattttttc
     6301 caccccaaaa actaaaaagc gatggtaagc gacgtatatt gattgctttt gataattcgc
     6361 tgttgctatt gcttaattca ctatcgacat caaggccttt ttgataacaa gctccaagcg
     6421 cccccaaaat tgtattgagc ttagcctgac tgcccatcca gtaaggattg taactcttac
     6481 ttcctgcttc cagacggttg atgacattca acaaggctac atgcttactg tagatagttt
     6541 tgagattctc gcaagacaga tctttgggga gattatgaat ctcacctttt gttctggttt
     6601 ctggttctgc tttggctctg gcagttcttt ttatctctgg atgaaactta tgattaagtg
     6661 cgcaatattc gattgcagca acctcatttt gagttctcaa aatatacacg aaattcgctg
     6721 catgaatgat gttttgtgca cttgcattaa caagtaaaaa ggcatagttt ccattttctt
     6781 ttccccaatt ggcatgggga acaagcagtc ccttaccact ggctgttaac ttcccattgc
     6841 tgtcagtcac ttgaaagtga ttggattgag aggaataact gacccaacct gatttcgcag
     6901 cctcatagta caggacttgc caatgaccag cccctccttg agtaccagtc cggaaaaaat
     6961 agacactgct tttattaaga gttgccagat ccttttccaa cgtttcctga tttttcccac
     7021 ttacccataa tgttttttct tcatctggac tgaacataaa agcagggccc aggtcaactt
     7081 gaatatcttg aacatctcga gtgtatgcaa aatcaagatc actgcctaaa atcaaccgag
     7141 cagctttctt tatcatcatt tctgcacaat tttggtttaa gaatacttct ttttttaaat
     7201 tcattggaga atcccagcca tttttggatt attataatac aaaataatca cataaaaagc
     7261 taagtgattg cttggattat agaaccatcc caatatttga gccccctgta agacagcaaa
     7321 aaggaacaaa gcaaggagtg ggaagtcttg ctcctttcct tgccagtcaa tgcctcctta
     7381 tgcttgataa gaaagaagaa aaaattgact ctgtggcccc gttcaggatc atgcccctgc
     7441 attaatacct caagagtcgt gttaaaatcc caaagataga agcttggttt aaaacagctt
     7501 gattggaatt ggttatgaaa gaaaagacag cgaaacaatt gaaattgttt attatctatc
     7561 taattagcta ttggctatta agcattattt catgccttat cgctttcggt tatgatgatt
     7621 ccctgcgcat gttgcttgcc tcgccaaaat cagatctcag cggtacttta ttatttttat
     7681 cttcatttat tgccgccgct ttgttgtttg tttttcgata caaaactttt tcagataaac
     7741 cttatcctta ttttattttt gggttctacg taggaaacgt ctcgttactg atgttattca
     7801 tattggatgc ttttattcgc gaactcattg tatggaaatt ccctgaattc ttgcttgttt
     7861 ttatatcacc attcgttgag ttagtgctct cctatctatt ttttggcttt gcattcctgg
     7921 caatcattcc ggcagttacc agtgccatta ttctctatgc agttcagaga aaattattat
     7981 tgccaccaat ataaatctcc agagtcttct ctttttcatt tgtcatcgct cccttgttct
     8041 cgcctggaaa aaaggaagcc tgcccctaac ctgttgcccg atttattgat tccccacaaa
     8101 aatatatttt ataataaaat gtatcatttt atattataat gctatattat gcgttttttg
     8161 gagaaaatat gggcatagta aaaatttcgg acgaattgca tgaagcaacc aaactgatgg
     8221 caagagccat gagccggtca atcaattcac aagcagaata ttggattaga atagggaaat
     8281 tggcagaaga aaatccctcc attacctata ctgacattct taaaatttta gtccatcaag
     8341 cttcactagg tgaaatatac gatgttagtg aagacgcctg aagcaataga aaaaatgcgc
     8401 gttgcggggc agttggctgc atccgtactt gaaatgatag agccttatgt cattgcaggt
     8461 accagtacca aggagcttga acaaatttgt cgtcagtata ttgtcgagga tttgaaagcg
     8521 attccctcga cgttaaacca ctatgggttt cccgcatgca tttgcacttc cataaatcac
     8581 gtggtatgcc atggcatccc ctcagagaaa aagcttaaaa atggcgacat cattaatatt
     8641 gatgtgactg tgcaaaagga cggttatatt ggggatacca gcaaaatgtt tctcgttggc
     8701 aacataaagc catttgctaa aaagttagta gaagttaccc aggaatgcct atacaaagct
     8761 atttcaatag tccgcccagg ggctcatctc ggagacatcg gaaacataat ccagacacac
     8821 gcagaacaac atggatattc aatagtacga gagtttggtg ggcatggaat tggtaaatcc
     8881 atgtgggaag agcctcacat tatgcacttc ggtaaaccaa ataccggatt acgattacaa
     8941 gcaggaatga ctttcactat cgagccaatg ctcaatttgg gccgtaaaga ggtcaagact
     9001 ttaggcgatg gttggacggt tgtcacaaaa gaccataaat tatctgcaca atgggagcat
     9061 accattttag tgaccgataa tggacatgaa atactgacct taagagcaga cgagcaccta
     9121 tagacgtctc tcctcttgct tggtaattac ggttcctcca agcctcgcga cggaaaacat
     9181 atactataat caaactaaag gaaacccagt caatcaaggg aaatagcatg ccttgcgaaa
     9241 aatgcgattc agtgacaacc taccatttat cgggaaccta ccagttactc attttacccc
     9301 cacgaggtca ttgctattca aaattattaa agcatttaac cgaaaataat tttgatatca
     9361 ataaaagaga cagtggaatt atcgaattat tatttactgc tgagagagca caagacatag
     9421 gtcatgcact ggagcgatgt tattcacaag cggaacttga ggacagcaaa gcagttttgt
     9481 tgccaattga tatcgcggac agaaacgtag agagagtact caatcatacg cattcattaa
     9541 aaaaaattgt gggcttgtgt ttgtctcaat ggttggttgg acttattgaa aatcaggggc
     9601 tatcaacctt ctgccagcct attttgcata ataccacact caaaccttat ggttttgagt
     9661 gccttcttag gggaaagcaa gataataata tactgccttc ctctgattta tttggagcag
     9721 caaaatcgag cgacatgctt tttttattag ataagaaagc acgtatatgc cacattgaaa
     9781 atatgagcaa aattgacatc gctgaaaaaa aaatcttcat taattttaac ccaacagcca
     9841 tttacgatcc ccttttttgc ttgacaacaa cgaaccaatc cttaaaaaaa acatccatta
     9901 aacctgcaca aattgtattt gaagtcactg aaagtgatga agtaaaagat aaaaaacact
     9961 tgttaaaaat aatagattat tacagaaatc aaggttactg tgtcgccctt gacgatttgg
    10021 gctccggtta cagctctttg aatttgctta ctgagttaca acctgattac gttaaacttg
    10081 atcaagaatt gattagccag attcatacaa atgaattcaa acaaatcata ttggaaaaaa
    10141 tatgtgagat ggctaacaaa ctaaagataa aaataatctg cgaaggcata gaaacagaag
    10201 acgagttaca gattataaaa aattatgcca tagattttta tcaaggtttt ttgtttgaga
    10261 aacctatgcc ttacgaaaaa atacccgact atatcaaagc aagcacacaa aaatgaattg
    10321 gctattggat atatgacaaa tgcttacaaa cattgaaaga gcaaaacttc aattaattga
    10381 tgaaaccagc gatatggtca ttataaccaa agcggaacca cttgatgcac ctaatggtcc
    10441 cgaagtggtt tgggtgaata aagcctttga aaaaatttca aaatataaag ccaatgaaat
    10501 tattggccaa acaccacgta tccttcaagg taaggaaacc gatcaaaaca cattaaaaat
    10561 gataaaagaa gcaattaaag ctcaaatgaa tatcaatgtg gatttattaa attatactaa
    10621 ggatggaaca ccttattgga ttaatttttc aatagtatat ttgagagacg ggcagggtaa
    10681 tttatgttat ttaggcgcaa tagaaaaaga catctccgca atcaagaatt taaatattct
    10741 attatctgaa aaagcaaaga ctgatccact taccaatgtt tacaatcagg aaaatttctt
    10801 cattgcaggg aatgaagcgg ttaaaaactt tcatcagaac caggaaacag ttggattatt
    10861 atttttcgat gttgatgatt ttaaaggaat aaacgatacg caagggcata tttatggcga
    10921 taagatatta aaaaaagttg cccaggaatc tttaaaactt gttcgtaaaa cggataacgt
    10981 atttcgatat ggcggcgatg agtttgctat tattttttca ggcattgacg tgaaaatcct
    11041 ggaaagaaaa gcacaacagc tacaaaaaaa gctggcaaaa tctcacatct ccgtgagcat
    11101 tggaggaacc atttcaaagc aaagtgacaa atcgataaac accctcttgg caagagcgga
    11161 tgaagcactc tattacgtca aagccaataa aaaaggatta ttttttatca gaaaataatc
    11221 acattttgac gctgccaaat tgcgaagaaa ttaaa
//
LOCUS       DBNCWK010000103        10810 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.103, whole genome shotgun sequence.
ACCESSION   DBNCWK010000103 DBNCWK010000000
VERSION     DBNCWK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.103"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1726
                     /locus_tag="AC06IO_002203"
     CDS             <1..1726
                     /locus_tag="AC06IO_002203"
                     /inference="COORDINATES: protein motif:HMM:NF012824.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_process: GO:0007264 - small GTPase-mediated signal
                     transduction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RasGEF domain-containing protein"
                     /protein_id="HIO9875379.1"
                     /translation="YCHLDCYYAEINYHSGTESFDQLVEHALAKKASDRKQEIEIIAH
                     ELKMLTIEFYKTVPLGEFHNGSRSSKKEEHLTPAITKFAGYFNRLNNYFLTKIITQPP
                     GNIVNAIQFLIQLAKSVCSLDTNNLPDLNSLMLISSILNGSIMNRLSNYFSMLTEQEK
                     DTIREINDIIAPDKNYKTMRSILSRREALPFLGLILSDLTFAMENETQLFQIETVGET
                     LTKLAQIKWLVNFEVVQYQTNLLQFLDNYQPSDEDMLYYKSLRLQPRKSDILSLDDFL
                     EDPAKVFTTLDEQYFNNNLIPNIVYRGELHPISQCGIQLTAWFYDKLCSFDHKTRDIN
                     ALESLLKSLENALKSFIKIYNQFYYPIYQSEQINQYHILLKLSQLKAEFEKISSIDPR
                     TCQNLTRPKIKRWFMSKEASIPSQIQDDPDRLNGPQEADEGIKIRGGSTILSPKNKSS
                     RVRFYSSPVIDAEKPAMRFFSSLSTDPIPSKNSPIIPSRAQRFSLPVELSDSNQTNDN
                     SVNLKESDELAKSTETVGRSKISPRRKKSQSYGFYSNPVQSKVTDSTKDLNVNNPSNT
                     PKVFLHSS"
     gene            complement(2040..2747)
                     /locus_tag="AC06IO_002204"
     CDS             complement(2040..2747)
                     /locus_tag="AC06IO_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875380.1"
                     /translation="MLGNNVKLANLRVLEFVATKLGDIRNDVVFLGGCTTGLFISDPL
                     VPDVRYTLDVDCIVDVISRNQYYQLEKKLNKQGFKKSITEDVICRWFYDDFILDVMPT
                     DETILGFGNRWYKQAIEFSNIYPLTDNLEIRVVTAPYFLATKFEAFKTRGKMDFYASH
                     DFEDIVSVLDGRIEIVDEIKNADSTLQEYLIKSLRKIMTSPSFKGAIPGHFSQYGSIA
                     NDRIELLEQKLTSIQSQ"
     gene            complement(2737..3264)
                     /locus_tag="AC06IO_002205"
     CDS             complement(2737..3264)
                     /locus_tag="AC06IO_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIO9875381.1"
                     /translation="MLKSQDCIVLIKLLANIGSNLSQRQLSAALGISLSEVNAGIKRL
                     EEAGLLRKDKQGKLFPNVHAAEEFLIHSIKFLFPVKLGEYTRGTPAAIAAPLFRDKIA
                     LGNDPIPVWPDAMGEERGVALLPIHPNLSKTLRANPDAAFHELLVLIDVIRSGRPRER
                     NLAAALLKEKLNHAG"
     gene            3711..4226
                     /locus_tag="AC06IO_002206"
     CDS             3711..4226
                     /locus_tag="AC06IO_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIO9875382.1"
                     /translation="MFNVSIRKATSLDAEAIASVHVHSWKKAYQQFIPEIVLDSLSIP
                     ERTQLWNELLNNGVTVLVLEIDNQIIGFASICTFRDDIEDNSKGEISAIYIHPNFWHQ
                     GFGKKLSLAALKNLKELGYTEALLWVLSDNHQARSFYEGMGFELNDKSKLTEFYDGGA
                     LLKEVLYKKKL"
     gene            complement(4558..4722)
                     /locus_tag="AC06IO_002207"
     CDS             complement(4558..4722)
                     /locus_tag="AC06IO_002207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875383.1"
                     /translation="MVTMLVPIGHKVPGINALANGLGHFHPSHAFPKLPFLFNPLRLK
                     ITGTWDGNPP"
     gene            complement(5115..5366)
                     /locus_tag="AC06IO_002208"
     CDS             complement(5115..5366)
                     /locus_tag="AC06IO_002208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875384.1"
                     /translation="MVKGLPSPLEYRNDLYYWPPNVAISPDTTNLFITMDGIDKVCFL
                     NTGLQGGLEQISEISIIINGAKKDWNCFPYTTTILEARP"
     gene            5814..6608
                     /locus_tag="AC06IO_002209"
     CDS             5814..6608
                     /locus_tag="AC06IO_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIO9875385.1"
                     /translation="MKKIKIYQVDAFASSIFSGNPAAVCVLDEWLSEEQMQFIASENN
                     LSETAFVVPNQGNFDIRWFTPATEVDLCGHATLASAYILFEKLGYKSSEIIFQSKSGM
                     LRVTGQNQQLQLDFPALPYHRITPTRVLLDALKIKPQEVYESTFDLLCVYPHESDVAE
                     AVPDLLALSQLPYRGIIITSLCGDGSVYSRCFYPACNVPEDPVTGSAHCVIAPFWCAR
                     LNQPLIRARQGLKRQGELLCELNGNRVLLTGTCHLYLEGNIYIPEI"
     gene            6610..7698
                     /locus_tag="AC06IO_002210"
     CDS             6610..7698
                     /locus_tag="AC06IO_002210"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIO9875386.1"
                     /translation="MKKIATYTIQDWLFSIANGRFDIDLAESGIQPHRIQDLSFTENY
                     DLNYSVDIGQEQLRQCIADMYEVDLARVMIANGSQEALYLFYRSFLSSADHVITFTPG
                     WQQSWEVPNYIGCAVTKIPLTRETGYKIDMAQIIDSLRPNTKLIVLTSPNNPLGITCH
                     PVDLEKLYELCCEKGIFLLNDEEYLTDYTQSIVQFGRLTGAVSSLSKIYGYPGLRVGW
                     FIGTPSLIQDMVNYKRYTTVTNSSLCEFLALEVLKNRKQYVSDYQAMSAQGESLLREF
                     VARWPECDMISPQGTPFAYITLPKTWNSAQFCQQLLAQHHVLIMPAEVFEDKHALRIS
                     FGRPQDLLRKGLEKLDEFINFYRVQYAN"
     gene            8109..9545
                     /locus_tag="AC06IO_002211"
     CDS             8109..9545
                     /locus_tag="AC06IO_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875387.1"
                     /translation="MTPLFPQKGQPIAIKQGGSGSCFLLACLDCLLNREADGADVVRK
                     KFKQTTSGVTLRIKRTDNTMKVQWEKMAGKYTYSYDPRTDEDVIFIDNEKLKEIDNSK
                     KGSSSNSLAVKILEHISAYYYQIAWDGESLLAHKLHPKFRNNPIAKQSDTTFLAKLLG
                     VHVTTFYDPRKLVKLKTIDPMIPVYVGMNFGKLDRLGRYRRHALRIGNIKPLEDGEYE
                     VTLINPWNNQKQTTCLLTALRKRKCHFAVFDSSPQKKLLTEIFLEYKTEIGCELFRNK
                     ELAFLLCEMKQCGLLDSELAKYPLSSCMVLYKKLPNLVANYRRLPYSERYNMLICVAN
                     SNGHVRLFNKLMQGSSVVDKPSVARLPQRVYPSAKSFFADLFASLEASLERVHYNDLL
                     KALRANYGHLRSVNTALNQVENRQGIYSKMELRNMNQRLERLGFFMKCHTGQRDHRHS
                     DLGQQPNFCDRSTENLNAKSHQDKGVSL"
     gene            9805..10551
                     /locus_tag="AC06IO_002212"
     CDS             9805..10551
                     /locus_tag="AC06IO_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875388.1"
                     /translation="MKRYIWFHPSYVLESSEATLLGQQTNAKDHGYILLNDQELGKVT
                     CEDHLTIMGHSTLAPTPAEDTGLYIQGDTADDLIRRLTTNGLKEAPKILSLESCRAGI
                     SGGLAQQLSRSPFFKYTLIEANLNSVGRKPMEMQWNFPTDSLGRAVLKLDNSPWMFYL
                     GGFLVGRLTHDTYTTEDLFSLLSPPTFHKHFFAHYQPGFFGGRSGRHCLFNKTTITLE
                     KASNFAHEDQDSASAKALEMVLKKLYSQPA"
BASE COUNT         3340 a         2057 c         2107 g         3306 t
ORIGIN      
        1 ttattgtcac ttggattgtt attatgccga gataaattac cactcaggta cggaatcatt
       61 tgaccaatta gtagaacatg ctctagccaa aaaagcatcg gatcgtaagc aagaaattga
      121 gataattgct catgaactta aaatgttaac catagaattt tataaaacgg ttcccttggg
      181 tgaatttcat aatggcagtc ggtcatcaaa aaaagaggag catttaactc ctgctattac
      241 taaatttgct ggttatttta atcgccttaa taattatttt cttacaaaaa ttattaccca
      301 accaccaggc aatattgtaa atgccataca atttcttatt cagttggcca aatcagtttg
      361 ttctttagac acgaataatt tgcctgattt aaacagccta atgctcattt caagcatatt
      421 aaatggaagc atcatgaatc gattaagcaa ttatttttct atgttaacag aacaagaaaa
      481 agatacgatt cgagaaatta atgacatcat cgcgcccgat aaaaattaca aaaccatgcg
      541 cagcatacta tctcgtcgag aggctttgcc ttttttagga ctcattctta gtgatcttac
      601 ctttgctatg gaaaacgaaa ctcaactctt ccaaatagaa actgttggag aaacactgac
      661 taaattagcc caaatcaagt ggttagttaa ttttgaagtt gttcaatatc aaacaaattt
      721 gcttcagttt ttggataatt accagccttc tgatgaagat atgctgtatt ataaatcact
      781 taggcttcag cctagaaaat ctgacattct tagtttggat gatttcttag aagacccagc
      841 caaggtattt acaaccctcg atgagcaata ttttaacaat aaccttattc ctaatattgt
      901 ctaccgtggg gagttacatc caatcagcca atgtgggatc cagttaaccg cttggtttta
      961 tgataaatta tgttcatttg atcataaaac ccgggatatc aatgcgttag agtcccttct
     1021 gaagtcgctg gagaatgcat taaaatcgtt tatcaagatt tacaatcaat tttattatcc
     1081 catctaccaa tccgaacaaa tcaatcaata tcatatactt ttaaaattgt ctcaacttaa
     1141 ggccgagttt gaaaaaatat cctctataga tccaaggact tgccaaaatt taactcgccc
     1201 aaaaataaag agatggttca tgagtaagga agcatctatt ccaagccaaa tacaagacga
     1261 tccggatcgt cttaatggcc ctcaagaagc tgatgaaggc attaagatac gtggcggatc
     1321 aacgattttg tcacccaaaa ataaatcgtc acgtgttaga ttttattcta gtcctgtaat
     1381 agatgcagaa aaacccgcaa tgaggttttt cagttctttg tcaactgatc ccattccttc
     1441 caaaaattca cccataatac cttcaagggc ccaaagattc tcactccccg tagagttatc
     1501 cgactctaac caaacgaatg acaattcagt aaatcttaaa gaatctgatg agcttgccaa
     1561 gagcacagag acggttggtc gatcaaaaat ttcacctcga agaaaaaagt cgcaaagtta
     1621 tggattttat tctaatcctg tacaaagcaa ggtaacagat agtacgaagg atttaaatgt
     1681 taataatcca agcaacaccc ctaaagtttt tttacacagt agttgatctt tggctatgag
     1741 tagaacttta agtttacttc gaatgatcct tctctcctga gggcaatgaa taaattgtag
     1801 tgattattaa aaatggttgg aaacaagttg agactgctca acatggtcgc agctaaaggt
     1861 ctagtggtct gtgggtttgt tcaggatctg acactgacct aaaataaaat ctgacagttc
     1921 tcgcagtata tttttgatgc tgggggcttc tgcaatatca ccgcttagta atacaccgtt
     1981 gctgctggtg ttaataattt cttggtaaag ttttttgcgc tcattagaca agtttgatgt
     2041 tactgactct gtatagaagt tagcttttgc tcgagtaatt caatccgatc attagcaata
     2101 cttccgtatt gagagaaatg gccaggaatg gcgcctttaa acgatgggga ggtcatgatt
     2161 tttcgtagtg atttaatcaa gtattcttgc agggttgaat cagcgttttt aatttcatct
     2221 actatttcaa tgcgtccatc caacacggat acaatatcct caaaatcatg gctggcgtaa
     2281 aaatccattt tgcctcttgt tttaaaggct tcaaatttgg ttgctaaaaa ataaggggct
     2341 gtaacaactc gaatttcaag attgtcagtc aatggataaa tgtttgagaa ctcaattgct
     2401 tgtttatacc agcgatttcc aaatcctaaa attgtttcat cagtaggcat tacatcaaga
     2461 atgaaatcat cataaaacca acgacaaata acatcttccg ttatggattt cttgaagcct
     2521 tgtttgttaa gttttttctc taactggtag tattgattcc tggaaataac atccacgata
     2581 caatcaacgt caagtgtata gcgcacgtcc ggtacgagag ggtcagatat aaataaacct
     2641 gtggtacatc ctcccaagaa aacaacatcg tttcgaatat cacctaattt tgtcgcgaca
     2701 aattctaaca ctcttagatt tgccaatttc acattattac ccagcatgat ttaatttttc
     2761 cttcaaaagc gctgctgcaa ggtttcgctc acgcggacgc cccgagcgta tgacatcaat
     2821 taggacaagc aattcatgaa aagcagcgtc ggggtttgct cgtagcgttt tggataaatt
     2881 agggtgaata ggtaataatg caacacctcg ctcttctccc atcgcatcag gccaaacggg
     2941 aatggggtca ttacctaagg cgattttatc gcgaaaaagt ggggcggcaa ttgctgcggg
     3001 tgtaccgcga gtatattcac ctaattttac agggaagagg aatttgattg agtgtattag
     3061 aaactcttct gctgcatgaa cattagggaa aagtttgcct tgtttatctt tccttaataa
     3121 ccccgcttcc tctagtcgct ttatgcctgc attaacttca gatagactaa tccccaatgc
     3181 agcggataat tgacgttggg ataagttact accgatattt gccaatagct ttattaatac
     3241 aatgcaatct tgtgacttta acatggtatt attctcaaaa tgacacaaat aaattatatc
     3301 acaggcgttc gctgttcgcg aacagaagaa catccagtta ttgggattta gttcaattcc
     3361 aagggtaaca cttaatgaat ggggcctaat tgggggcttt ttcaaaaacc acatttaaat
     3421 aattatttat tatcaatcaa ttagatggcc aatattatgg agtgaccact aaagactatg
     3481 acaaacccta ataaatcaag agattaaagc gtatttttcc caaatacggg gggtatttta
     3541 tcaagcacat aacactctaa ctcctaatcg gtagtttttt ctgtattgtt ttaagattga
     3601 gtcatgttac agagggatta attggttggt atactgtccc ttttatcata aaaatggtaa
     3661 aacaaattaa attattcgag acccaaagct aaataaaggg gcatcaattc atgtttaatg
     3721 tttctattag aaaggctact tctttggatg cggaggcgat tgcatcagta catgttcatt
     3781 cttggaaaaa agcttaccaa caatttattc cagaaatagt acttgatagt ttatcaattc
     3841 ctgagcgtac ccagctatgg aatgaactac taaataatgg cgtaacagtg cttgtattgg
     3901 aaattgacaa tcaaataatt gggtttgcga gtatttgtac atttcgtgat gacattgagg
     3961 ataattctaa aggggaaatt agtgctattt atatacaccc caatttttgg catcaaggat
     4021 ttggtaagaa attgtctctc gctgcgttaa aaaacctgaa agaactaggc tataccgagg
     4081 ctttgctttg ggttttatca gataatcatc aagcgcgtag cttttatgaa ggcatgggct
     4141 ttgaattaaa tgataaatca aaacttacgg agttttacga cggtggagcc ttattaaagg
     4201 aggtgctgta taaaaagaaa ttataatcgc aacatatcat attcttaaaa tgatgggttt
     4261 tttgactgtg taaggagaag aatatagctt ttctatttgg atcttaactg gttgtaaaac
     4321 tttggtcaaa aatcaaagtt ttttgctaca tccagaatat tcttacgcaa atatataaaa
     4381 cagacatgcc tattattgga attataacaa agccaagaaa caggtgaatc tgtgttctgt
     4441 tctctgatga ggaactgtaa aattttttga attttttaaa aattgacatt caatataccc
     4501 ttgcaactta tttaattgta tatctattta aatctctact tttgatgcta attaaaatca
     4561 ggggggatta ccatcccagg taccagttat ttttaatctt aatggattaa acaggaaggg
     4621 taatttgggg aatgcatggc ttgggtggaa atgccccaag ccattagcta gggcattaat
     4681 tccagggacc ttgtgtccaa taggtactaa cattgttacc atgttgatcg taactctccc
     4741 ttgcagagcg ctcttcggat ttaccaaaca aactacctgg atgtttggct gaagttgtgc
     4801 tcgttagtgg acgacggccg tcctcagtgt agctcaatct agggtcttga gcttcgggag
     4861 caatatgttc ttgttcttta ctgtttttta taggtttcat tatctatcct caggttaaaa
     4921 gttatttggg tacaaattaa agtcaaaaag ccatccaaac attctcaatg gcaaaattat
     4981 gaaataattg tactttaaaa aaacaattga gccatggttt aaaaggacaa tttacggtga
     5041 taaagggaca atgtgagctt atccaaacaa gagtagatac gggagccacc tattcgattg
     5101 gtgctttggt atttctatgg gcgtgcctca agaatagtcg tggtatatgg gaaacagttc
     5161 caatcctttt ttgccccatt gatgatgata ctaatctcgg aaatttgctc taacccaccc
     5221 tggagtcctg tattcaagaa acacaccttg tcgataccat ccattgtaat gaataaatta
     5281 gtagtgtcag ggctgatagc aacatttgga ggccaataat aaagatcatt tctgtactct
     5341 aaaggagacg gcagtccttt taccataata gttgtcccaa aagactcagt gcagatccac
     5401 actaatacta aaaatagggc gagtttcata aattcttatc ccttaatatg ataatgagat
     5461 tgagattgcc taagggcatc tctcacatac tcctatacca tataggatag actattatgg
     5521 cttggtgtat ctgatttgaa ctcctgagat ggggataaat aagtgctcaa tacgaggcaa
     5581 atccctggct gagctgtggg ttataaattc aaggtgaatt tttcgtacct ggctatcaga
     5641 attttgtttt cactcactta aaccttccca ttagcagggt tgattccccg gatattgatc
     5701 cgactttcaa tttcacaata aagcatggca gttggctggt caattacata gcttgctata
     5761 tattagcaag ctatgttaac ataaaatatg ttttatgaac aaatgagctg agtatgaaaa
     5821 aaatcaaaat atatcaggtg gatgcgtttg cttcttctat ttttagcggt aaccccgcag
     5881 ctgtatgtgt cctggacgaa tggttaagcg aggaacaaat gcaattcatt gcctctgaaa
     5941 acaacctgtc tgaaaccgca ttcgtagtgc cgaatcaagg aaattttgat attcgctggt
     6001 tcacacccgc tactgaggtg gatttgtgtg ggcatgccac tttggcaagt gcttacattt
     6061 tatttgaaaa attgggctac aaaagttcgg aaataatttt tcaaagtaaa agcgggatgc
     6121 ttcgtgtgac cggacaaaat cagcaattac aattggattt tccagctttg ccttaccacc
     6181 gaattacacc gaccagagtg ttattggacg ctttgaaaat aaaacctcaa gaagtatacg
     6241 aaagcacatt tgatttatta tgtgtttatc cgcatgaatc ggacgtggca gaggcagtcc
     6301 ctgatttact tgcattaagc caattaccct atcgcggcat tattattaca tcactatgcg
     6361 gggatggttc agtatactct cgctgttttt atcctgcctg caatgtacca gaggatccgg
     6421 tcacaggctc tgctcactgc gtcatagcgc ctttttggtg tgctcgtttg aatcagcccc
     6481 ttatcagagc aaggcaaggg ctaaaacgcc agggcgaatt attatgcgaa ttaaatggca
     6541 atcgcgttct tttaacgggc acttgccatt tatatttaga aggtaatatt tacatcccag
     6601 agatttaata tgaaaaaaat agcgacttac actatacaag attggctttt ttccatcgcg
     6661 aatggccgat ttgacattga tttggcagag agtgggatcc aaccacatcg cattcaggac
     6721 ctttcattta ctgaaaatta cgacttaaac tacagtgtgg atattggcca ggaacaattg
     6781 cgtcagtgca ttgcggatat gtatgaagtt gacttggcaa gggtaatgat tgccaatggt
     6841 agtcaggagg ctttgtattt attttaccga tcatttttat catccgctga ccatgtcatc
     6901 acgtttactc ctgggtggca acaatcctgg gaagtaccaa actacattgg ctgtgcggtt
     6961 acaaaaatcc cattgactcg ggaaacaggc tataagatcg atatggcaca aattattgat
     7021 tcgttaagac caaataccaa gctcattgtt ttaacctccc ctaataatcc attaggcatt
     7081 acttgtcatc ctgtcgattt agaaaagtta tatgagctgt gttgtgaaaa gggtattttt
     7141 ttattgaatg acgaagaata tttaactgac tacacgcaaa gcattgtcca atttggaagg
     7201 ttaacaggtg ctgttagtag tttatccaaa atttatggtt atcccggttt gagggtcggc
     7261 tggtttatag gaaccccttc tttaatccag gatatggtta attacaagcg ttatactaca
     7321 gtgacaaatt catctctttg cgaatttttg gcgttagaag tgcttaaaaa cagaaaacag
     7381 tatgtgtctg attatcaagc aatgagcgca caaggtgaga gccttttaag ggagtttgtc
     7441 gcacgatggc ccgaatgcga tatgatttcg cctcaaggta cgccttttgc ctatatcacc
     7501 ttgcctaaga catggaattc cgcccaattt tgtcagcaac tgctcgcaca gcatcacgtg
     7561 ttaatcatgc cagctgaagt ttttgaagac aaacatgcct tgcgaatttc atttgggcgc
     7621 cctcaagacc tattacgtaa aggtctggaa aaactagacg aattcattaa tttttaccgg
     7681 gtgcaatatg ccaattaaat tactttccaa aatattagta tcgcaagctt tgcctatcaa
     7741 aatgccctta aacatgggct gggatcttcc caagatttat tctaaaggac tagatgggca
     7801 ctattcgttt tgcttttgcg agttctccca tcccttagcc ttactcgaat aaagattttc
     7861 tcggacaacc ttatcagaaa tgaggttatt taatgggagt gatgtatttc tacataagct
     7921 aaggcgagca tggcttgaat agcccagatt tcatattcac aatacgtcag ataaaatggt
     7981 tggggttcag ttttttcctg ccttattgtt ttttggcttg caaatgccct aagtgcgcat
     8041 tattttgata aatttgagtc ttcggttatc atatacagca cctgaagcaa ctcgtatatt
     8101 tgtaaccaat gacgccatta tttcctcaaa aaggacagcc catagccata aaacaaggcg
     8161 ggagtgggtc ttgctttctc ctggcttgcc tggattgttt gctcaatagg gaagcagatg
     8221 gggccgatgt tgtacggaaa aaattcaaac aaacaacgtc cggcgttaca cttagaatta
     8281 aacgcactga caacaccatg aaggtgcagt gggaaaaaat ggctgggaag tatacttatt
     8341 cttatgatcc cagaactgat gaagacgtta tttttatcga taatgaaaaa ttaaaagaaa
     8401 ttgataacag taaaaaagga tccagttcca attcgctcgc agtaaaaatt ttagagcaca
     8461 tcagtgctta ctactaccaa attgcctggg atggcgaaag tcttttggct cataaactac
     8521 acccaaaatt tcgcaataat cctattgcca agcaaagcga tactacattt ctagccaagc
     8581 ttttgggtgt tcatgtcact actttctatg acccgcgcaa gctggttaag cttaaaacaa
     8641 tagaccccat gattcctgtt tatgtaggga tgaactttgg gaagctggat agattgggca
     8701 ggtacaggcg tcatgcacta cgtattggaa atattaagcc tttagaagat ggagagtatg
     8761 aggttacttt aatcaatccc tggaataatc agaaacaaac cacctgtctg ctcactgctc
     8821 tgaggaaaag gaaatgtcat tttgccgtat ttgacagctc cccacaaaaa aaattattaa
     8881 cagaaatttt tttggaatat aagaccgaga taggatgtga gttatttcgc aacaaagaat
     8941 tggccttttt gttatgcgaa atgaaacaat gtggtttatt ggatagcgag cttgcgaaat
     9001 atccgctaag ttcatgtatg gttttgtata aaaagctccc caatttggtt gcaaactaca
     9061 gacgccttcc ctatagcgaa cgatacaata tgctaatatg tgttgctaac tccaatggcc
     9121 atgtgcgttt attcaataaa ctaatgcaag ggtcgtcagt agttgacaag ccatctgtgg
     9181 cgaggctgcc acaacgtgta tatcccagtg caaaatcatt ttttgctgac ttgtttgcat
     9241 cgctggaagc gtctttggag cgggtgcatt acaatgattt actaaaagca ctaagagcca
     9301 actacggcca tttgcgttct gtcaatacgg cgttaaatca agttgaaaat agacaaggga
     9361 tctactctaa aatggaatta aggaacatga atcagcgtct tgagcgcctc ggtttcttta
     9421 tgaagtgcca taccggccaa agagaccatc gtcactctga ccttggacaa cagccaaatt
     9481 tttgtgaccg gtcgactgaa aacttgaatg ctaaaagcca tcaagataaa ggtgtttcgc
     9541 tttgatatcc aggaacctgg aaatatctgt aaagagccag aataatgcaa tgggaggagg
     9601 ggctgcaagc ctcggttttg gggtgccacc aatataaaat agggccaagc ctttcttgta
     9661 agcttggcct cacaagttat tttcctgagc tgggcacatt attttaaatt tgaaaagaca
     9721 acaaatatcg aattttacat ttacaaaaaa caaataaatt tgtatcgttt gagttcatct
     9781 tgtttaataa aagagacttt ctctatgaag cgatacatct ggtttcatcc atcctatgtt
     9841 ttagagtctt ctgaggcaac tttgcttggc cagcaaacca atgccaagga tcatggttat
     9901 atcttattaa acgatcagga attaggaaaa gtcacatgcg aagatcatct gacaatcatg
     9961 ggacattcca cacttgcacc aacaccagcc gaagataccg gattgtacat acagggggat
    10021 acagcagatg atctaatcag acgattaact actaatggtt taaaggaagc ccccaagata
    10081 ttgagccttg aatcttgtcg agcaggaatc tctggtggcc ttgcccagca actgtcacgc
    10141 tctccttttt ttaaatacac gttgattgaa gcgaacctaa acagcgtagg tcgaaaaccc
    10201 atggaaatgc agtggaattt tccaacggac tcattaggtc gcgccgtatt aaaactggat
    10261 aacagccctt ggatgtttta tctgggggga ttcctggtcg ggagactcac tcatgatacc
    10321 tataccacgg aggatttgtt tagcctcctg tctcccccaa cttttcataa gcatttcttc
    10381 gcccattatc aaccaggctt ttttggagga cgatccggac gccattgttt gtttaacaag
    10441 accacaataa cattggaaaa agcatcaaat tttgcccacg aagatcagga ttcagccagc
    10501 gccaaggctc ttgaaatggt cttgaaaaag ctttattctc aaccagctta attcaacatc
    10561 aacatgactt acgaaaaaaa tcactctttt tgttaaaaat gtctttaatt tggccatttg
    10621 gtttatctaa gctcccttat tagcaatatt ttttgccttg accttggggt ttttcgtaag
    10681 tcctgagcaa tagactttta gtcagactca cctcaataat attaaggaac aatatgatct
    10741 aacacatgat tatgcaaaat gacttttgag agaaacctgg ttaaaactga aaataattat
    10801 tttttcatga
//
LOCUS       DBNCWK010000104        10785 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.104, whole genome shotgun sequence.
ACCESSION   DBNCWK010000104 DBNCWK010000000
VERSION     DBNCWK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.104"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(221..1510)
                     /locus_tag="AC06IO_002213"
     CDS             complement(221..1510)
                     /locus_tag="AC06IO_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIO9875389.1"
                     /translation="MSFYIQLASLSIIILYVLLLFRNVDPLVATAVCVGLGFLWNIST
                     PIDIGNAMADALGSFMALVGFIIMLGRGLGEVLTHTQVSHTLVHQIVYGVGVNTQRRA
                     KIGIVLSSFIIVGLLGTLAGGLAILAPSLRPIAGSVGLSRPSLAVLMQASAEEALILG
                     PFAPPVVTLLGVTGLSYGTVFLYASLPIAVVTLITTWFMASRLQKLYINEPCEDENST
                     EPFTPNRQQKRATLIFLISFLFCVVYGLFTQAKTSYVIFVMLFLALITGLSCRLGLKQ
                     IFNLLFEGMQKSLHLFFLFILFDPFMALIHQAGGFNALTELLTPLINLGGKPVLSILI
                     GFTGAFGMPGAAEATIKMLHQLFSPSVLQMQLPLVTFALCMLFATRVTNYAYPGANMF
                     AAMGFAGSENVKAMIQNGLVVTGVQVAFLIVYSLFIP"
     gene            complement(1510..2613)
                     /locus_tag="AC06IO_002214"
     CDS             complement(1510..2613)
                     /locus_tag="AC06IO_002214"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HIO9875390.1"
                     /translation="MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVVLIKTDCGKIG
                     YGSAASTPAITGDSKESIIAAIKSALGPPLIGHNIAEFNLLLQMTDEAIGGNSSAKAA
                     IDIALHDLFAQYCGLPLYRLLGGNTNCIDSCITISVKEVDEMVSDAVDLIQRGHKTLK
                     IKLGLNPEDDINRIRAIRQAVGNDITLLADANQGWSYEEALIAITAFKKENLNVPLVE
                     QPVNARNLPDLKTISDQVGCAIIADEACFSPEDALNIAKINACDGVNIKLMKSGGIEK
                     AQSIYHIAQAAKMQIMVGCMLESPIGVAAIASFALSKPDISYADLDPIFLIRENYILG
                     GAQSLSNKIILADKPGLGIEGITQGLNLIGVIR"
     gene            2916..4166
                     /locus_tag="AC06IO_002215"
     CDS             2916..4166
                     /locus_tag="AC06IO_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HIO9875391.1"
                     /translation="MLFKLNKSSSHKPEQISRSYHCLMLQLKTWETNHYTPDLNEEYF
                     RTLDIETRNVQIFYNDFVNNVLIPLEQQPTTDLPEFITQEDIFKLLSFKIFMPLAPIF
                     AEREQWMKRIAGTDGKNAHASESYNKFKKIFLNSHHKFVIEESLSHFEHGTLKYHHEW
                     LTAKRQIAKEVKSLRALQENWKEKKNKIDRKKAAALLDFLDNFESIPNRLRSREEEGF
                     KRTEVYKKALSTDKLKAIELLNQHLFDYSSPQEELAERMKLIKALYENNILFEQHHTP
                     IGWLKNQLNIETVLDKKLASLEQSLKAAENALNSYMEHRIPKTATISTELRKEPEKDS
                     IALPEENKSPEAVSENQNTSTSNPEIQPQTNPVQNRKNTGKVYLKVQLYEENLRRLGV
                     FTPKTPPCSELLKKDLTDGNRISL"
     gene            4358..5938
                     /locus_tag="AC06IO_002216"
     CDS             4358..5938
                     /locus_tag="AC06IO_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIO9875392.1"
                     /translation="MFDLAKLTEFNHALVYGFEPESSPARLAGSETFEDALFPEAGQH
                     QDMLNAYLYAQETILPQIKKTGLSHITPEMLIEWVKAIHGFIGKSLMQAYDRKSGEYT
                     NEIIFRWHLGAELGVHFTLYLSGLHECKSPQQLARYLNKQFDMDYQSALDFIALLEKI
                     AKDKKYTIHESLQPYIDYSSPGIKGILVQNKLASAYNLNQLTESEKITVNKILKICMF
                     PPLIPEAMNRWAQETVGQLRSCDSRDLKNVSEFLATTFYQLTEVHPFANANGRTATCL
                     INTFLRAFGYPSIVLRHPGERENKDSRYQKAFAEIDSSLVPLIDLIYTRVNEAQEKAF
                     ENEKLKKLIVLRVALSDLLQETKSKHPQFNLIAFQKQVFSSPETLFAMQMADETDASI
                     FVLSMSINKLSQIAKELDLSKQKKPTLFSTAVLDSTQINAVIKSLEKISGLDGWKTNA
                     KKGLVTWLELPDMEKAKEIASELEALKVAKITVARRADNKIPVIKCEEIDCEKLVNAA
                     LQKDGKTSSKNAEFVAMF"
     gene            6206..8188
                     /locus_tag="AC06IO_002217"
     CDS             6206..8188
                     /locus_tag="AC06IO_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1959 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875393.1"
                     /translation="MLISTRTLEPALSHFNDRNNLIKQLDLSSDSDNNSFYKWVLAFW
                     LGSNITREKIIKHPLYIKCEAFIDLGYSCILVLDKQMSLLKQNSLAYKILQKNLFEII
                     QYYNVNYPLLTQNPQALFSFFANILAKIDSKVCEDISHRKILELTQNRCLAELSRTQN
                     GPPDGLAATQVSNDVTSLMKVNPFNLGVAINKLITENFSKELFFPHYIKPTTDKHITH
                     KLLIPAWDGIVLEGLSVKGRKTTNNTVVLALIGHFQTEHHYLNTSFHEFQELFGTELV
                     LINHRNYSNRSNKFANSAEEIARDVIAFANHFRQKNKKIVLYGMCGGAAHMILAAHML
                     SHQKIPFKLIVDRFSQKYINFVDFKTLSRARDFSHSNGQDCSRLLPGYKYYPGLMPYL
                     LILLFLLLFILVQLGLFLTKTNIDFSKLVRRIPEEDLLILQAKGEKIAGLKRPLYTDM
                     IVHPENDMRAAVKDRRKYRKTILKNLCEHCLHAAGQAVFSAEIQKIFLQLFNCFDQCL
                     QLINNEKLMENTITNRPVDLHSKKLYTLTTRNKLPISQFIRGFFKQSPKMHAHLLDSI
                     KPYSSHLIVDALKQIYGNHPSMHSNLLQFSNHLALLLNDMKTNQFFISYMADRLSATQ
                     LADLNEPINALLRSELLQLIFKSSSEQNNHNHRVSI"
     gene            8373..9146
                     /gene="lirA"
                     /locus_tag="AC06IO_002218"
     CDS             8373..9146
                     /gene="lirA"
                     /locus_tag="AC06IO_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LirA"
                     /protein_id="HIO9875394.1"
                     /translation="MFFSKDEKNPIKRALQGELLQNEPFIQLCTKIESYLMDTEAVNE
                     QLIELNEQLTMRLKEKELKPGEKGATKQLRTLIQEILTEAGFREGMLQTIGNKPLKKE
                     DFMFLVSSGFMLKDSSLRASSHGELTHAIQWCLIILKQKKDSSFLENIPTSEICDRIY
                     KKLGHQDSSNPNYPFTCWDVLIDKLGEIDSRSPEWLSDHIQNDEDQIFPVLREVIKNR
                     TEKGKTEENKGKLQKKLENPPEHYEKHEEIENILMPKPK"
     gene            complement(9183..10691)
                     /locus_tag="AC06IO_002219"
     CDS             complement(9183..10691)
                     /locus_tag="AC06IO_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HIO9875395.1"
                     /translation="MNDSENESDSEYDSDSEYEYELRTLPSKIHFVWVGGPIRAQYLR
                     TIMDVATVALKSGFEVNLWVDNEMNYIKTSTQEGIDIPNLRIRNIHELEEGMRNDPFY
                     EGDRYKKFWEYVNRERVGFRNLAAAADFFRFEILRQEGGYYFDTDIVFYLDENSQFVA
                     DEVAFGIKAHVDLHTGSLPDPRGGVSAHYVTTLGNVNNDIIAVIPEHEVMEDAILNAM
                     QRYEEYDSSSLPLESFKKRFGPYGNLMDAKRSPYQLEGAGDFRKHQTIAAGPGALQDA
                     LRDYMQDVEVDDYNLLVTLKVGNQHSKVADDKDKKPIMGIEVVSKCDKTWLTKKRKAA
                     QKAFDTDSIAIEPSLSKESGHAVSKGESYSDEEEYSDELTEEAGFHGNLRKEQFIHEL
                     KDYIITKTEEARQALKVKENAEGYDSEENILSKHFKTRHIEITKGFSVEQKIAAVEKL
                     ISLLQEKDGAEPLNAIDYAALTSSRLGKLVERYDDLILKEYVVPDEYGEKPY"
BASE COUNT         3686 a         2162 c         1825 g         3112 t
ORIGIN      
        1 tttcattttg aaaaaaactg tgagcttaaa gcaaactcaa aaactcccat gatagataat
       61 agaataaaac atattaaata caaatttgac ttgctcaatt tagctttggc agaaattttt
      121 aaacactttt ctttaccagc ttctgcatct atataagccc taagtatagc cctgtgtggt
      181 gcaaactcat tgatttaaag gattaaagcg atcacaaaca tcagggtatg aataaactat
      241 aaacaattaa aaatgcaacc tggacaccag tcactacaag gccattttgt atcatggctt
      301 tgacattctc acttccagca aaacccattg ccgcaaacat attggcgcca gggtaagcgt
      361 aatttgtgac acgagttgca aagagcatgc acaaagcaaa agtgacaaga ggcaattgca
      421 tctggaggac agaggggcta aagagctggt gaagcatttt gatggttgct tctgcagcgc
      481 ctggcatacc aaaagcgccg gtaaatccta ttaagatcga taatactggt ttccctccca
      541 ggttaattaa tggtgttaat aactcagtta atgcattaaa tcctccagct tgatggatta
      601 gtgccataaa aggatcaaat aaaataaaca aaaagaagag atgcaagctt ttttgcattc
      661 cttcaaataa caggttaaaa atttgtttca aaccgagtcg acaggataat ccggtaatca
      721 gcgccagaaa taacatgaca aagattacat aagaggtttt cgcttgagta aataagccat
      781 agacaacaca aaacaaaaag gaaatcagaa aaattaatgt agcccttttt tgttgtctat
      841 tgggagtaaa aggctcagtg ctgttctcgt cttcgcaggg ctcattgatg tataattttt
      901 gtaatcgtga agccataaac caggtggtaa ttaaagtcac tacagcaatt gggagcgaag
      961 cgtaaagaaa tactgtccca taacttaatc cagtgacacc aagcaaggtt acaaccgggg
     1021 gagcaaaagg ccccagaatt aaagcctctt cagctgaagc ttgcatgagt accgccaaac
     1081 tgggacgtga caagcctact gaccctgcaa taggacgcaa actgggggcc aggatagcta
     1141 aacctcctgc taatgttccc agcaaaccaa caataataaa ggatgagagg actataccaa
     1201 ttttagcgcg tcgctgagta ttaaccccga ctccataaac aatttggtgc actaaagtat
     1261 gacttacctg ggtatgcgtt aaaacttcgc ccagccctcg acctaacatg atgataaaac
     1321 caaccaaagc cataaatgaa cccaaggcat cagccatggc attccctatg tcaattgggg
     1381 tgcttatatt ccacaaaaaa cctagcccaa cgcaaacagc tgtcgctact aatggatcaa
     1441 cgttacgaaa taacagcaaa acataaagaa taataatgga taaactggct agctgaatgt
     1501 agaaagacat tagcggatca ctccaattag attcagtccc tgtgtgatgc cttcaatccc
     1561 taatcccggt ttgtctgcca ggataatttt attgctgaga ctttgtgccc caccaagaat
     1621 ataattctcg cggatcaaaa aaataggatc gagatctgca taagaaatat cgggcttact
     1681 taatgcaaaa ctggctattg cagcaactcc aatcggcgac tccagcatac atccaaccat
     1741 tatttgcatt ttggccgctt gagcaatatg gtagatagat tgagctttct caatgcctcc
     1801 tgatttcatc aactttatat tgaccccatc acaagcatta atctttgcga tattcaatgc
     1861 atcttctggt gaaaaacacg cttcgtcagc aataattgca caaccaacct gatcactaat
     1921 cgtctttaag tcaggcagat ttcgagcatt aaccggttgc tccaccagag gaacattcaa
     1981 attctctttt ttgaacgcag taatggcgat taatgcctct tcataagacc atccctgatt
     2041 tgcatctgct agcagagtaa tgtcattgcc tactgcctga cgaatagctc gaattcgatt
     2101 gatgtcgtcc tctggattta atcccaattt gattttcagg gttttgtgcc ctcgctgaat
     2161 taaatcaacc gcatcactca ccatctcatc aacctctttg acacttatcg taatacacga
     2221 atcgatacaa ttggtatttc ctcccaacaa tctatagaga ggcaaaccac aatattgggc
     2281 aaataaatca tgcaaagcga tatcaatagc agccttcgct gacgagttac ctcctatagc
     2341 ctcatcagtc atttgcagta ataaattgaa ttcggcaata ttatgtccga taagaggagg
     2401 ccccaaggca cttttaatag cagcaataat ggattccttg ctgtctccag ttatagcagg
     2461 tgttgatgct gctgaaccat aaccaatctt tccacaatcc gtttttatta ataccacaac
     2521 atcatcaacg cattctgtac gtctcaccgc ggtaataaac ggacggctta atggaatagt
     2581 tagttctgcg atatggattt ctttaatatt caaacaagca cctgttcatt tattgtttgc
     2641 ggggtaaccc taatgagata ttaaaatatt aagagttaat ttggcaaaac atgtttttaa
     2701 attatatctc tcaccaaata atattaactt gataccctgc ttcaatacat ctcttttcta
     2761 ttcttgaatt aactcaataa aataaaccaa ctatttctga agaacaccaa aaacagatac
     2821 ggatttcatg tattcaaaaa caactgtctt ttgctatcat ccaaatttct tatcatcccc
     2881 aaaatcaatc catttgtatt aacctggaga tacccatgct ttttaaactc aataaatcca
     2941 gctctcacaa acctgaacaa attagcagga gttaccactg tttgatgctt cagttgaaaa
     3001 catgggaaac aaatcactac acaccagact taaatgaaga atattttagg actttggata
     3061 ttgaaacaag aaatgttcaa atattctaca atgattttgt gaacaatgta ttaatccctc
     3121 ttgaacagca acctacaact gatttgccgg aattcatcac ccaggaagat attttcaaac
     3181 tcttaagctt taaaatattt atgccattgg ctccgatatt cgcggagaga gaacaatgga
     3241 tgaagcggat tgccggtact gatggaaaaa atgctcatgc cagtgaaagc tacaataaat
     3301 tcaaaaaaat ctttttaaac tctcatcata agtttgttat tgaggaaagc ttatcccatt
     3361 ttgaacacgg cacattaaaa tatcaccatg agtggctaac tgcgaagcgg caaattgcca
     3421 aggaagtcaa gtcattacgt gcattacaag aaaactggaa agagaaaaaa aacaaaattg
     3481 acagaaaaaa agctgctgcc ctattagatt ttctggataa ttttgaaagt attcccaaca
     3541 gattacgctc aagggaagag gagggattca aacgaacaga agtctacaaa aaagcacttt
     3601 ccacagacaa gctcaaagca atagaactcc tgaatcagca tctttttgat tacagcagtc
     3661 ctcaagaaga attggcagaa agaatgaagc ttatcaaagc tctgtatgaa aataatattc
     3721 tatttgaaca gcaccatact ccaataggat ggctgaaaaa ccaattgaac attgaaacgg
     3781 tacttgataa aaagctggcg tctctggagc aatcgttaaa agcagcggaa aatgctctaa
     3841 attcttatat ggaacataga atacctaaaa cggcgacgat ttccactgaa ctaagaaaag
     3901 agccggaaaa ggatagcatc gcgctccctg aggaaaataa atcacctgaa gcagtttcag
     3961 aaaatcagaa tacaagtact tctaatccgg aaattcaacc tcaaaccaat cctgtacaaa
     4021 atagaaaaaa caccggcaaa gtttatctga aagtacagct gtatgaagaa aacctccgta
     4081 gacttggtgt atttactcct aaaacccctc cttgctccga gttattgaaa aaagatctta
     4141 ctgatggaaa tagaatctct ctataaaaat aagcgaactg ccttttatag tctttgtgaa
     4201 aggactcaca aatagctatt gagaatgacc taaaaagtac aaaagcttct gtaagcgaat
     4261 acttaaacct cgacaaagcg caattaaagt acatggtgga tatttattgc gttctctgct
     4321 acgatcattc cccttttaat taattacaaa aacaaccatg tttgatttag caaaactgac
     4381 tgaatttaat catgctttag tgtatggatt tgaaccagaa tcaagccccg cacgtttggc
     4441 aggctcagaa acatttgaag acgctctttt tccggaggca ggccagcatc aagatatgtt
     4501 aaatgcctat ctttatgcac aagaaacaat tcttccgcaa attaaaaaaa cagggttatc
     4561 tcatattacc ccagagatgt taattgagtg ggttaaagcc atacatggct ttatcggaaa
     4621 atctctaatg caggcttacg atagaaaatc aggtgaatat actaatgaaa ttattttccg
     4681 atggcacctg ggtgccgaac tgggggttca ttttactttg tatttgtcag gtctccatga
     4741 gtgcaaatcc ccacaacaac ttgcaagata tttaaataaa caatttgata tggattatca
     4801 gtctgctttg gattttattg ctctgttaga gaaaattgcc aaagataaaa aatatacaat
     4861 tcacgaatca ctgcaaccct atatcgatta ttctagtcct gggataaagg gaattcttgt
     4921 tcaaaataaa ttagcgagtg catataacct caaccaattg acagagagtg agaaaattac
     4981 agtcaataag attttaaaaa tttgtatgtt ccctcctctg attcctgaag ccatgaatcg
     5041 atgggcgcaa gaaacagtgg ggcaattacg ctcttgtgat tcaagggatt tgaaaaatgt
     5101 ttctgaattc ctggcaacca ctttttatca gcttacagaa gtgcatcctt ttgccaatgc
     5161 taatggaaga acagcaacct gtcttattaa tactttcctt cgcgcatttg gctatcctag
     5221 cattgtgctt cgccaccctg gggaaagaga aaataaagac agtcgatatc aaaaagcatt
     5281 cgctgaaatt gattcctctc tagttccgtt aattgatctg atttatactc gtgtcaacga
     5341 agctcaagaa aaagcttttg aaaatgaaaa actaaaaaaa ctgatcgtat tgcgagtcgc
     5401 tttaagtgac ttgttacaag aaaccaagtc taaacatcct cagtttaatc taattgcatt
     5461 ccagaaacag gtcttttctt ctcctgagac cttgtttgca atgcaaatgg ctgatgagac
     5521 tgacgcatcc atttttgtcc tgtcaatgag tatcaataag ttatctcaaa tagcaaaaga
     5581 attagacctg tcaaaacaaa agaaaccaac attgttttca acagccgtgt tggatagcac
     5641 acaaataaat gccgtgataa aaagcctgga aaaaatatct ggtctggatg gctggaaaac
     5701 caacgcaaaa aaaggattag tcacttggct ggaacttccc gatatggaaa aagccaaaga
     5761 aatcgcctct gaattagaag cgctcaaagt agcaaaaatc accgtggcac gacgagctga
     5821 caataaaatt ccagtgatta aatgcgaaga aattgactgc gaaaaacttg ttaatgcggc
     5881 tctccagaaa gacggtaaaa catcctcaaa gaacgcagaa tttgttgcga tgttttgata
     5941 aaaaatgagc ctgaattaaa gaggcaggtc aaaaacaaac aataaattac agagagggga
     6001 aaaaacagca ggagaaataa tttgttttaa tttaagcttg atctggccaa tgccagtttt
     6061 ttataaaact gcggccggat cttattgagc caaaacgaca ggaaatactg agcacaattt
     6121 tttcatagca ccccaaaaat gacatctatc cattttgatc gagatagaac ttaaatgaac
     6181 tttcttttat tacgtttttg aacttatgtt aatttctacc agaactctcg agccagcatt
     6241 aagtcatttc aacgacagga ataatttaat caaacaatta gatttgtcat ctgatagcga
     6301 taacaattcc ttttacaaat gggttttggc gttttggttg ggttcaaaca tcactcgcga
     6361 aaaaattatc aaacaccctt tgtacataaa atgtgaagct tttattgatt tgggttactc
     6421 gtgcattttg gttttggata aacaaatgag cttgttaaag caaaattcac ttgcttataa
     6481 gatcctgcaa aaaaacttat ttgaaataat tcaatattat aatgtcaact atccgttatt
     6541 aacccagaat ccccaagcac tgttttcttt ctttgccaat attcttgcaa aaattgacag
     6601 caaggtatgt gaagatattt ctcaccgaaa aatccttgaa ctgactcaga ataggtgcct
     6661 agctgaatta tcgcgtactc aaaatggccc gccagatgga ttggcagcaa cacaagtgtc
     6721 taatgatgtt accagtttaa tgaaagttaa tccgttcaat ttaggtgtcg ctatcaataa
     6781 gctgattacg gaaaatttta gcaaggaact ttttttccca cactacataa agccaacaac
     6841 cgacaagcat attactcata aattattaat accagcctgg gatggtattg tacttgaggg
     6901 gctttccgtt aagggaagaa aaacaacaaa caataccgtt gttttagcgt taattggaca
     6961 tttccaaaca gaacatcatt atcttaatac cagctttcat gagttccaag agctttttgg
     7021 cacagaatta gtgcttataa atcatagaaa ttattctaat cggtccaata aatttgccaa
     7081 cagtgctgaa gagatagcca gggatgtgat cgctttcgca aaccatttca gacaaaaaaa
     7141 caaaaaaatt gtactgtatg gaatgtgtgg cggggctgcg catatgattt tagcggccca
     7201 tatgctatcc catcagaaaa tcccatttaa gttaatcgtt gacagattct ctcaaaaata
     7261 catcaatttc gtagacttta aaacactttc gagagccaga gatttttcac actcaaacgg
     7321 acaagattgc tcgagattgc ttccaggtta caaatattat cctggtttaa tgccatactt
     7381 gctcattctc ctttttttat tgttgtttat tttagttcaa ttaggtctat ttttaacaaa
     7441 aacaaatatt gatttctcaa aacttgtgcg gaggattcct gaagaagacc tcctgatttt
     7501 acaagccaag ggagaaaaaa tagcgggctt aaaaaggcca ttatatacag acatgattgt
     7561 acaccctgaa aatgacatgc gagctgccgt aaaagacaga agaaaatata ggaaaactat
     7621 cttgaaaaat ttatgtgagc actgcttgca tgctgctggt caagctgttt tttcagccga
     7681 gatacaaaag atttttcttc aattatttaa ctgttttgat caatgcctcc agctgataaa
     7741 caacgagaaa ttaatggaaa ataccatcac taatcgccct gtagatttac acagtaaaaa
     7801 actttacacg cttacaacca gaaataagtt acctatcagt cagttcatta gaggtttttt
     7861 taaacaatca ccaaaaatgc atgcccattt gcttgattca atcaaaccat actctagtca
     7921 ccttatagtt gatgctttaa aacaaattta tggaaaccac ccatcaatgc actctaattt
     7981 attgcaattt tctaatcatt tggctttgct tttaaatgat atgaaaacca atcagttttt
     8041 tatttcttat atggccgatc gtttatctgc tactcaatta gctgatttaa atgaacctat
     8101 aaatgcgctg ttaagaagcg aactacttca actaattttc aagagttcgt ctgaacaaaa
     8161 taatcataat catcgggtat ccatttaatg cattcattga ccccactaat tgggttatcg
     8221 ttgatactaa atggcaagat taatgtaaat gcgcttaaac catgtgctca tcaacgccca
     8281 actcaactaa taagcgagca ataaagaata tttataaaca atatttatta aaaataatct
     8341 atacttaaaa aaattagcgc gttggaggta atatgttctt ctctaaagat gaaaaaaatc
     8401 caataaaaag agctcttcaa ggtgaattat tacaaaatga gccttttata caactatgta
     8461 caaaaataga aagctacttg atggataccg aagctgtaaa cgagcaactt attgaattaa
     8521 acgaacaact gacaatgagg ttaaaggaaa aagaactcaa acccggagaa aaaggagcaa
     8581 caaagcagtt aagaacactc atacaggaaa tactaaccga ggctggcttt agagagggaa
     8641 tgttacaaac aatagggaat aagccattaa aaaaagaaga cttcatgttt ttagtatcat
     8701 caggatttat gttaaaagac agcagtctaa gagcatcatc ccatggtgag ctgacccatg
     8761 caattcaatg gtgtttaatc atattgaaac aaaagaaaga cagcagtttt cttgaaaaca
     8821 tacctactag tgagatttgt gaccgaattt ataaaaaact agggcaccaa gattcttcga
     8881 atcctaatta tccttttact tgttgggatg tacttatcga taaattagga gaaatagact
     8941 ccagatcccc ggaatggctt tccgatcata ttcaaaacga cgaggatcaa atttttcctg
     9001 tgttaagaga ggtaataaaa aacagaactg aaaaaggaaa aacagaagaa aacaagggaa
     9061 aattacaaaa aaaactggaa aatccgccag aacattatga aaaacacgag gaaattgaaa
     9121 atattctcat gccaaaaccc aaataaattt gaactactac agaccaacta atccatttga
     9181 attcagtatg gtttttcccc atactcatca ggaacaacat attctttaag tatcaaatca
     9241 tcgtatcttt cgactaactt acccaggcga gaactggtaa gcgctgcata atcaattgca
     9301 ttaagcggtt ccgccccatc cttttcctgc agtaaactaa taagcttctc tacggcagca
     9361 attttctgtt caacagaaaa tccttttgtt atctctatgt ggcgtgtttt aaaatgcttg
     9421 ctgaggatat tttcctcaga atcatagcct tctgcattct ctttcacttt caaagcctgc
     9481 cttgcttctt ctgtttttgt aataatgtaa tctttcaact catgaataaa ttgttcctta
     9541 cgcaaattac catggaatcc agcttcttct gttaattcat cactatactc ttcttcatca
     9601 gagtatgact ctcccttact tactgcgtgc ccggattcct ttgaaagtga gggctctata
     9661 gctattgaat cagtatcaaa agctttttgc gcagcttttc tttttttagt cagccatgtt
     9721 ttatcacact tactgaccac ttcgattccc atgatcggtt ttttatcctt atcatctgct
     9781 acttttgagt gttgattacc aacttttaaa gtaactagta agttgtaatc atccacttca
     9841 acatcttgca tgtaatcccg cagtgcgtct tgcaacgcac caggaccagc agcaatagtt
     9901 tgatgctttc taaaatcccc tgctccctcc aattgataag gactgcgttt agcatccatc
     9961 agattgccat atggaccaaa tcttttttta aaagactcta gaggcaaaga ggatgaatca
    10021 tactcttcat aacgttgcat agcatttaag attgcgtctt ccatcacctc atgctcagga
    10081 attacagcaa taatatcatt gttgacattt cctaaagtag tgacataatg agcagatacc
    10141 cctccgcgtg gatccggcaa tgaacctgta tgcaaatcca catgggcttt tataccaaaa
    10201 gcaacttcat ctgctacaaa ttgtgagttt tcatccaaat aaaaaacgat atcggtatcg
    10261 aagtaataac cgccttcttg tcttaaaatt tcaaaacgaa aaaaatctgc cgctgccgca
    10321 agatttctga aaccgactct ttctctattg acatattccc aaaatttttt gtacctgtcc
    10381 ccttcgtaaa aaggatcatt tctcatccct tcttctaatt catgaatatt ccttattctt
    10441 aaattaggta tgtctattcc ttcttgagtt gatgttttaa tgtaattcat ttcattatca
    10501 acccataaat taacctcaaa accacttttt aatgctactg tagcaacatc cataatggta
    10561 cgtaaatatt gggctcgaat aggtccccca acccaaacaa aatgtatttt ggatggtagc
    10621 gtacggagct catattcata ttcagaatcg ctatcatatt cagaatcact ttcattttca
    10681 gaatcgttca tgaaactctc gcataataac tcaatattgt ttaattatag tacaccccta
    10741 tctaattcag ccgaaactct gagtttcatt taaaaaccat tttaa
//
LOCUS       DBNCWK010000105        10727 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.105, whole genome shotgun sequence.
ACCESSION   DBNCWK010000105 DBNCWK010000000
VERSION     DBNCWK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.105"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            217..1959
                     /locus_tag="AC06IO_002220"
     CDS             217..1959
                     /locus_tag="AC06IO_002220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIO9875396.1"
                     /translation="MTHDTELSELMQAVAKKSLQIMTDFKEKPIPLSSLVSQYIDLTE
                     HFQNLIAVILKNPEKVWQMQLNYLEDALSLAQAQFNYWLEGKPLPINDQRFNGEDWIN
                     NPFFNLLSQHYLLANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANFIHTNPQ
                     LMAETLQSHGKNLLRGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRN
                     SMMELIQYCPRTTKVKSIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWV
                     NPDETYANKSFYDYLNEGPREAISVIQKQLRVKQVNTLGFCIGGTLLASLLAYNKATK
                     DLSIRSATFLAAMIDFSDPGDIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRA
                     NDLIWSFFIKNYLRGKSPVPFDILYWNADSTNMPATMHSQYLRWMYLHNNLIKPGKIR
                     LNHIPVDVTNIDIPTFFLSTQKDHIAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINP
                     PTQQKYGYRTNNSMDLSAEQWFEKSKEHSGSWWPEWLNWLKLHSGRLINSPDINHLPF
                     APIMDAPGSYVLKK"
     gene            2008..2391
                     /locus_tag="AC06IO_002221"
     CDS             2008..2391
                     /locus_tag="AC06IO_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875397.1"
                     /translation="MRKKNRILLASLFSLGSLNAFAAQDLTVVNTSDDLGYVTIGSYS
                     CELTSSCAIPPDNSIRINSDIVEAACANTPADCEIVFGVDSIKARIATGSFSLNEGIQ
                     YIAQSRWLNYSAKRLSKTVAEIYKR"
     gene            complement(2478..2897)
                     /locus_tag="AC06IO_002222"
     CDS             complement(2478..2897)
                     /locus_tag="AC06IO_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIO9875398.1"
                     /translation="MKLIWIVLALILAFVVILYLFPQINFREMPKGLVEGQLPDDKPN
                     WVSSLVNESDSHYIAPLKVESMPQLVACIERKIPELKIQKQEASFLVGYRQSPFFHFV
                     DWLCIHADGNVISSATMGYSDFGKNRELVEKIRSLCL"
     gene            complement(2898..5099)
                     /locus_tag="AC06IO_002223"
     CDS             complement(2898..5099)
                     /locus_tag="AC06IO_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIO9875399.1"
                     /translation="MMTKTNEENLTWKEKQKQRARAWIKVFLEKWFRIEVKGQYQANS
                     HSVIIANRASIIDVLLLSVFLPERLTLALHPDMFKKIWVKTLLFFADVIVVDPGSAQA
                     TRILIRAIRAGKRCLLFPQGLLRQQEDSLKVFEGPGLILQKVGAEVIPVRIEGAEHSI
                     FSNSKNKHRIRIFPKITLHILPAQKLSQAENTGVDRGGIRLRLFQLISDLSFANSFKP
                     YVLFSALIEGVSIGTKNKTKIEDNNCIPLTYRQFLARCFILGRQIKKQTAVGETVGVM
                     MPTTIAGMVTFFALQAYRRIPAMLNFSMGFYNLYSACNTARIKSIYTARQFIETAKLE
                     PLVEELQQAGIRIHYLEDFKSTIHLGNKLSGIFKGFFPTLSYRFLGDKISPDATGIIL
                     FTSGSEGKPKGVALSHANILANCWQMTSRVDFTPRDVLFNSLPIFHCFGLTAGSVLPL
                     VNGLNCFFYPSPLHYKVIPGLVYQTGATILFGTDTFLTGYARAAGKHDFKSVRYIFAG
                     AEKVKPETIRYWSEAFGARIYEGYGATEASPVISLNCPLASVPGSVGMILPFMDFKVE
                     PVEGIVQGGRLKLCGPNIMLGYLDEDKPGVIIEPEDGWHDTGDIVTVNADGFITIAGR
                     AKRFAKIAGEMVSLTAVEGIAASIWPELLHAAVVQKSPRKGEQIFLFTEAEYADKVSF
                     IKKVKERGQSELLVPHMIYPACQIPVLPSGKIDYITLEKQFLEQKETMETV"
     gene            5254..5937
                     /locus_tag="AC06IO_002224"
     CDS             5254..5937
                     /locus_tag="AC06IO_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIO9875400.1"
                     /translation="MRVLNSCNKVRGIFTLTQKTIKTFCFILAILVLIIFAVVFHNHS
                     LEIIDYIDDLGWLAPVLFLITYCLATLLLLPTMVLTLAGGAVFGPVFGTLLNLLGATS
                     GAAFAFLITRHLVYDWFSTKKGEKLNKLIAGVDEKGWVFVAFLRLFPIVPFNLVNYGL
                     GVTGISFRLYLLTTFIFLIPAEIIYTYFGYVGMDALTRPGHLYRNGGIFLTGLAIFFL
                     LVIRIMKKR"
     gene            complement(5964..6779)
                     /locus_tag="AC06IO_002225"
     CDS             complement(5964..6779)
                     /locus_tag="AC06IO_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIO9875401.1"
                     /translation="MPTRVIEDKGDMTPSFGIDDRIFLGEGLFETIRVNSSKPSFAYM
                     HWERLGNSARQLGIPFEISFDDWFEHLIQKIQKDNLYHGGIKAILSGGPASRGLAERG
                     QVSQLIFQTFNYSIQKHPVRLISINWLRDKANPLYQLKSVNYLEAIIAQRQAIAVGAD
                     DALFFNTENHVTETTCANLFLIENNILYTPRVEDGILPGITRARLISHCQQHKMSVQE
                     ISLTKKRIEDADAVFLTNSLQGIRRVLSLDNIIFEVNHPIIDKLIFLLNQDES"
     gene            complement(6793..6978)
                     /locus_tag="AC06IO_002226"
     CDS             complement(6793..6978)
                     /locus_tag="AC06IO_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA3496 family putative envelope integrity
                     protein"
                     /protein_id="HIO9875402.1"
                     /translation="MSDLFEEDEEEVIEVVDDFDDVEVDTEIIEAPGVSLDARRRLEN
                     MLEEKRLRDELDDFVDY"
     gene            7225..7806
                     /locus_tag="AC06IO_002227"
     CDS             7225..7806
                     /locus_tag="AC06IO_002227"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIO9875403.1"
                     /translation="MPDQYKVALRNTIKQVRSKLSVSYRTASSNQICTRIRSLEQYRQ
                     AKRIALYFAVNGEVDLTFLWKSAPLQGKYCYFPVLNNDSTLSFLPATPNTPFKTNKYD
                     IPEPDVDIELALPVDELDLIIMPLVAFDAHCTRLGMGSGYYDRTLSNKSKAHLLGVAY
                     QFQRVDYIEPRPWDVPLDAVITQKAIYWRNPLA"
     gene            complement(7816..8154)
                     /locus_tag="AC06IO_002228"
     CDS             complement(7816..8154)
                     /locus_tag="AC06IO_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIO9875404.1"
                     /translation="MKMITAIIKPFKLDDVHEALMEIGVPGITISETRGFGRQKGHTE
                     LYRGAEYVVDFLPKIKIELALPDNMVEAAIEAICKSAYTGKIGDGKIFVYALEQVVRV
                     RTGEVGTDAL"
     gene            8299..8553
                     /gene="ubiK"
                     /locus_tag="AC06IO_002229"
                     /gene_synonym="yqiC"
     CDS             8299..8553
                     /gene="ubiK"
                     /locus_tag="AC06IO_002229"
                     /gene_synonym="yqiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HIO9875405.1"
                     /translation="MFDPKQFDELANKLFATLPTSLQNFEKDIQQKFKEVLQSTFSRM
                     DLVTREEFDVQCKVLARTREKLEQLQHQLEDFIKRQEIKK"
     gene            8625..10136
                     /locus_tag="AC06IO_002230"
     CDS             8625..10136
                     /locus_tag="AC06IO_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIO9875406.1"
                     /translation="MSLAFTKTRSTIGIVAQPVSVEVHLSNGLPSFTMVGLAETAVKE
                     SKDRVRSAIINSQFEFPCRKITVNLGPANLPKTGSGFDLPIALGILAASEQIPLTNLA
                     NHEFIGELALSGELRGVSAIIPAVLAAHKDNQHLIIANANAAEASLTGHPKVFTANNL
                     REVCDYLCQGTSLQNLPPKPDLLFNNHELDWSDIKGQHHAKNAMVIAACGGHSILLSG
                     APGSGKTMMAKRFSTLLPELSETQALECAAINSIRGKLPDFREWRLPPFRAPHHTASP
                     VALVGGGNPPKPGEISLAHHGVLFLDELPEFNRQVLETLREPLESGHICISRAAAQIE
                     FPAKFQLIAAMNPCPCGQWGNSQANCLCTPDRISRYLAKLSAPLLDRIDMQVTIHALS
                     QEELIKPNTRLEKQSPAIREKVTKMHEIQMARQDSLNANLNSKTCEMVCELGSEEQLF
                     LREVMSKLKLSARGYHRLLKVSRTIADMSECNKVMLNHLQQALSYKQILHLPK"
     gene            10241..10627
                     /locus_tag="AC06IO_002231"
     CDS             10241..10627
                     /locus_tag="AC06IO_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875407.1"
                     /translation="MRSIRLLFPCILLMIPWQFATANRPTDNALAIIKSCYDESVNLS
                     NEFTPCVTKKLKTMPNAEDFQLIMNFKTDEKGTNERIPVFMVDKTGYMYYCIVTTGKT
                     LIINACAGEQGKPLTQGQMLSIDLPE"
BASE COUNT         3362 a         2207 c         1908 g         3250 t
ORIGIN      
        1 ttattgataa ggaaataagc aaataaaatc ttctgttctg ccttggcgat ttcatacttt
       61 ttaatatttt ttacaatagg ttagtcatca agggaatggg cttgccaata ttccatctgt
      121 ctataatgtg ctctggattt aatatagaat actcttgata aacacaacca aacccataag
      181 aataagctat tcgattattt gctttcagga gccaggatga ctcacgatac tgaactaagc
      241 gaactgatgc aggctgtagc taaaaaaagc ctgcagatta tgaccgattt caaagaaaaa
      301 cccatcccgc tttcatcgct ggtcagtcaa tacatcgatc tcacggagca tttccaaaat
      361 ttgattgccg tcattctaaa aaatcctgaa aaagtatggc aaatgcagtt gaattacctc
      421 gaagatgcct taagtctggc tcaagcacaa tttaattatt ggcttgaagg aaaaccactg
      481 cctattaacg atcagcgttt taatggggaa gattggataa acaatccttt ttttaacttg
      541 ctaagccaac attatctttt ggcaaatgag catatgaatt cattgctgga aaatatggaa
      601 tatggtgacg agaatctggc aaaaagagta cgttttttca cccgacaata tcttgatgct
      661 ttatccccag ctaactttat tcacacgaat cctcagctca tggcagaaac attacaaagt
      721 catggtaaaa acctattacg tggattacac aatttgctat ccgatgttga agcaggatct
      781 tccagattaa tcattaaaat gactgatacg gaagcattta aaatcggtga gaatctggct
      841 acaacgcctg gtaaagtcat atttcgcaat tccatgatgg agttaatcca atattgcccc
      901 agaacaacaa aagtaaaatc cataccttta cttatcgttc ctccctggat taataaatac
      961 tacattttag acttaagccc gcataattca ttaatacgct ggttggtcga acaagggatt
     1021 actgttttta ttatctcctg ggtcaaccct gacgaaacgt atgccaataa aagcttctat
     1081 gattatttaa atgaaggccc cagggaagcc atttcagtaa tccaaaaaca attgcgtgtt
     1141 aaacaagtca atacgctggg attctgtata ggcgggacac ttctggcttc tcttttagcc
     1201 tacaataagg caactaagga tctttccatt cgcagtgcaa cctttttagc ggcaatgatt
     1261 gatttcagtg atcctggaga cattgctgtt tttattgatg agcagcagat taataaactt
     1321 gaagaagaaa tgaaatccaa aggttatctg gctggcaaat ttatggcgtc cagctttaat
     1381 tccttaaggg caaatgacct aatatggtca tttttcatca aaaattacct gcgcggtaaa
     1441 agccctgttc cttttgacat actctattgg aatgctgatt caactaatat gcctgcaacc
     1501 atgcattctc aatatttacg ttggatgtat ttgcataata acttgataaa acctggaaaa
     1561 attcgactta atcatatccc tgttgacgta acaaatatcg acatacccac tttctttctg
     1621 tctactcaaa aagatcatat cgccccctgg aaaacaacat ataaaggatt tgaattaatg
     1681 aaggggccaa aacgttttgt tttaggtgga tctggacata ttgcaggaat tattaatccg
     1741 ccaacccagc aaaaatatgg ttatagaacc aataacagca tggatttatc tgctgagcaa
     1801 tggtttgaaa aatccaagga acactccggt tcatggtggc cagaatggtt aaattggctt
     1861 aaattgcatt ccggaagact catcaattcc cctgatatca atcacttgcc attcgcaccg
     1921 ataatggatg ctccagggag ttatgtacta aaaaaataat attttgaact caataaccat
     1981 acattaaaac ataaaaggag ttgtattgtg agaaaaaaaa acaggatact tttggcaagt
     2041 ttattctcgc taggcagcct gaatgccttt gctgctcaag atctgacggt tgttaatacg
     2101 tccgatgatc tgggatatgt gacaatagga tcctattcct gtgaattgac ttccagttgt
     2161 gctattcccc ctgataattc gattcgtatt aactctgata tcgtagaagc agcttgtgcg
     2221 aataccccag ctgattgtga aattgttttt ggggttgaca gtataaaagc aagaatcgcg
     2281 accggaagct ttagtttgaa tgaggggata caatacatcg cccaatctcg ttggctgaat
     2341 tattctgcta agcgtttatc caaaaccgtg gctgaaatct ataaaagata attcgattta
     2401 ttctctgctg atgtcctggt tgaagcacac agcaatagcc aggacaaggt gcaatgacat
     2461 ggattgatta agataaatca taaacacagg gatctgattt tctccaccaa ctctctgttc
     2521 ttgccgaaat cagaataccc catggtggca cttgaaataa cattcccatc cgcatggatg
     2581 catagccaat caacaaaatg gaaaaagggg ctttgtctat accccaccag aaaggacgcc
     2641 tcctgtttct ggattttcaa ttccgggatc ttgcgttcaa tgcatgctac caattgaggc
     2701 atactctcaa ccttcaaagg agcaatatag tgagaatccg attcattaac caggctactt
     2761 acccagtttg gcttatcatc tggcaattgc ccctcaacta accccttcgg catttctcga
     2821 aaattgattt gaggaaataa ataaagaatg acaacaaagg ctaaaataag ggctaatact
     2881 atccatatta atttcattta tactgtttcc atggtttctt tctgttctaa aaattgcttt
     2941 tcaagcgtta tataatcaat ttttcctgaa ggcaggacag gaatttggca tgccggataa
     3001 atcatatgag gaaccagcaa ttctgattgc cctctctcct taaccttttt tataaatgat
     3061 actttatctg catactctgc ttcagtaaat aagaaaattt gctccccctt tcttggagat
     3121 ttttgcacaa cagcggcatg taataattca ggccaaattg aagctgcaat tccttccacc
     3181 gctgtaagag ataccatttc accagcaatc ttggcaaaac gtttggcgcg acccgctata
     3241 gtaatgaatc catccgcatt gacggtgacg atatctcccg tatcatgcca accatcttca
     3301 ggttctataa ttactccagg tttatcctca tcaagataac ctaacatgat attgggtcca
     3361 cataatttta aacgcccgcc ttgtacaatt ccctctactg gttctacttt gaaatccata
     3421 aaaggtaaaa tcattccgac actacccggt actgatgcca acggacaatt aagcgatatg
     3481 actggcgatg cctcagtagc accatagcct tcataaattc ttgctccaaa cgcttcgctc
     3541 cagtatctaa tggtttccgg tttcactttc tcggcgccag cgaagatata gcggacgctt
     3601 ttgaaatcat gtttgcccgc agcccttgca taacccgtta gaaaagtatc tgtgccaaac
     3661 aaaatagtag cccctgtttg ataaaccaat ccaggaatta ccttataatg aagaggggaa
     3721 gggtagaaaa aacaattcaa accattaacc aaaggaagca cacttcctgc tgtaaggcca
     3781 aaacaatgga atataggtaa ggaattaaat aaaacatctc gtggagtaaa gtcgactctg
     3841 gaagtcattt gccaacaatt agccaatata ttggcatgac taagagcaac ccctttgggt
     3901 tttccctcag aaccagaggt aaacaggatt atgcctgttg catcagggga tattttgtca
     3961 ccaagaaaac ggtagcttag agtaggaaag aagcctttga atatcccgga aagcttgtta
     4021 cctaaatgaa tggttgattt aaaatcttct aaataatgaa ttcttatacc cgcctgttgt
     4081 aactcttcta ccaagggttc taatttggcg gtttctatga attgtcttgc ggtataaatt
     4141 gatttaattc ttgctgtatt acaagctgaa tataagttat aaaaacccat gctaaaattt
     4201 aacatggcag gtattcgtct ataagcttgt aaagcaaaaa aagtgaccat gccagctata
     4261 gtagtcggca tcattacccc aacagtttcg cctacagccg tttgcttttt aatttggcgc
     4321 ccaagaataa aacaacgagc caagaactgt ctgtaagtta atggaataca attattgtct
     4381 tctattttag ttttgttttt agttcctata cttactcctt caattaaagc agaaaataaa
     4441 acatatggct taaaactatt ggcgaatgat aaatcgctta ttagttgaaa taaacgtaaa
     4501 cggatacctc ctctgtcgac acctgtattt tcagcttgac tcaatttttg agcaggtaat
     4561 atatgaagag taattttcgg aaaaatccgt attcgatgtt tatttttgct attagaaaaa
     4621 atactgtgct ccgccccttc aatacgaaca gggattacct cggcgccaac cttttggagt
     4681 atcaatccag gtccttcaaa aacctttaag ctgtcttcct gctgtcttaa tagcccttgt
     4741 ggaaatagaa gacaccgctt gccagcccgt attgccctta ttaaaattct tgttgcctga
     4801 gcgctgcctg gatcaactac tatgacatca gcaaaaaata ataacgtttt tacccatatt
     4861 tttttaaaca tatcaggatg taaagctaaa gttaatcgtt cgggtaaaaa tacagaaagg
     4921 agtaacacat caattattga agccctgttg gcaataatga cagaatggct gttggcctga
     4981 tattgtcctt taacctctat gcgaaaccat ttctccagaa aaacttttat ccatgcccta
     5041 gcccgctgtt tttgtttttc cttccaggtc aaattttcct catttgtttt tgtcatcatg
     5101 tcgcatcagt tatctctata ttgactttta ttctgcaaga ttcaaaacga tttgaataga
     5161 taggtttatg aatcatattg atttcattta aaaaacttaa gcgaaagcaa catctgtttt
     5221 tatgataatt tacccggaat attgagagaa ggcttgcgag tccttaattc ctgtaataaa
     5281 gtgaggggca tctttacttt aacacaaaaa accattaaga cattctgttt cattttagcc
     5341 atattagttt taattatttt cgcagtggtc tttcataatc attcgttaga gatcattgat
     5401 tatatagacg atctgggttg gcttgcccct gttcttttct tgatcaccta ctgccttgcg
     5461 actttattgc ttttacccac aatggtatta actctggctg ggggagctgt gtttgggcct
     5521 gtatttggta ccttgcttaa tttattggga gccactagcg gcgctgcgtt tgcatttctg
     5581 ataacaagac acctggttta cgattggttc tcaaccaaaa aaggtgaaaa actaaacaaa
     5641 ttgattgccg gggtagatga gaaaggttgg gtttttgtgg ctttcttgcg attgtttcca
     5701 atagtccctt ttaacctggt caactatggc ttaggagtaa ctggcatatc atttcgtctt
     5761 tatttgctaa ctacttttat ttttctaatt cctgcagaaa ttatttatac ctattttgga
     5821 tacgtcggca tggatgctct gacaaggcca ggccatttat ataggaatgg tggaattttt
     5881 ctaactggat tagccatttt ctttttactt gttatcagaa taatgaaaaa aagataattg
     5941 gtttttcaat taataaaaat ttatcaagac tcatcctgat ttaataaaaa gatcaactta
     6001 tcgataattg gatgattgac ttcaaaaata atgttatcca gggagagcac ccttctgata
     6061 ccctgcagac tattagttaa aaaaacagca tcggcgtcct caatcctctt tttcgttaat
     6121 gatatttcct gcactgacat tttgtgttgc tgacaatgcg aaatgaggcg tgccctggta
     6181 atgccgggta agatcccatc ctctactcgc ggagtataca aaatgttatt ttcaatcaag
     6241 aataaattgg cacaagttgt ttcagtaaca tgattctctg tgttgaaaaa taaggcatca
     6301 tctgccccaa ccgcaatcgc ttgacgttga gcaataatcg cctctaaata attcactgat
     6361 tttaattgat aaagaggatt ggccttatcc cttagccaat taatactgat taaccgaaca
     6421 ggatgctttt gtattgaata attaaaagtc tgaaatatta attggctgac ttgtcctcgt
     6481 tctgccaagc ctctcgaagc aggaccacca ctcaaaatgg ctttaatgcc accatggtac
     6541 aggttatctt tttggatttt ttgaataaga tgctcaaacc aatcatcaaa tgaaatttca
     6601 aaaggaatac ccaactgacg agctgaattg cctagtctct cccaatgcat ataagcaaag
     6661 gaaggcttgg aactattgac tctaattgtt tcaaataatc cctcacctaa aaaaattcga
     6721 tcatcaattc caaatgatgg cgtcatatct cctttgtctt caataacacg agtaggcatt
     6781 atgatctcat aattaataat ctacaaaatc atctaactca tctcgcaatc gcttttcctc
     6841 caacatattt tccagtcgtc tgcgcgcatc caaactgaca ccgggagctt ctataatttc
     6901 agtatccact tcaacatcat caaaatcgtc gacaacttca attacttctt cttcatcttc
     6961 ttcaaacaag tcactcataa cattccctca aagaattaaa agtaacatta aataattgct
     7021 taaatggaac aagattccag gacggctata taccttattt ttaatttttt gcatagtatt
     7081 ttttaattat tttatgttat aaacttatcg catcgcgcat ttataatcac ccaatgttta
     7141 aaacaacata ttgatacctg gaaacacttg aactttaagc ccaatccatc taataataaa
     7201 aaagaaaatt agtgatacca aatcatgcca gaccaatata aagttgctct aagaaatacc
     7261 ataaaacagg taagatccaa gctttctgta tcctacagga cagcatcttc caatcaaatt
     7321 tgtactcgta tccgctcgct ggaacaatac aggcaagcaa agcgaatagc cttgtacttt
     7381 gcggttaatg gagaagttga tttaacattt ctctggaaaa gcgcgcctct ccaggggaag
     7441 tattgttatt ttccggtatt aaataatgat tccactcttt cttttttacc agccaccccc
     7501 aacacccctt ttaaaacgaa taaatacgat atcccggaac cggacgttga tattgagttg
     7561 gctttgcctg ttgatgaatt agacttgatt atcatgcctc tggttgcttt tgatgcacac
     7621 tgtactcgtt tgggtatggg ctcaggctac tatgatcgca ccttgtctaa taaatcaaaa
     7681 gcgcatctct taggagtcgc ctaccaattt caaagggttg attacataga accacgccct
     7741 tgggatgttc ccctggatgc cgtaataact caaaaagcta tttattggcg aaatcccttg
     7801 gcctaaaatg acattctata gagcgtctgt accgacttcc ccggttctaa ccctcactac
     7861 ctgttccaaa gcataaacaa aaatcttgcc atcaccaatt ttaccggtgt aggcggactt
     7921 gcaaatagct tctatggctg cttcaaccat attatctggc aatgccagtt caattttaat
     7981 tttagggagg aaatctacca cgtactccgc acctcgatat aattcggtat ggcctttttg
     8041 acgcccaaat cctcgagttt cagatatggt aattcctggc acccctattt ccattaaagc
     8101 ttcatgtaca tcatccagtt taaatggttt gataatcgcc gtgatcattt tcatattttt
     8161 ttccattacg ttaaatgcag agccacccat gatatttcta acacacaatt tttatgttat
     8221 tgcttttcaa gacatcttgg gtatagttgt aatccaacag ttttgctaga ctgtattcaa
     8281 aaataatccg gagccaccat gtttgacccc aagcaatttg atgaattagc taataagctt
     8341 ttcgctactt tacctacgag tctacaaaat tttgaaaaag acattcaaca aaaatttaaa
     8401 gaagtactgc aatcaacatt cagccgaatg gaccttgtta cccgtgaaga atttgatgtg
     8461 caatgtaaag tacttgccag aactcgagaa aaactggagc aattgcagca ccagcttgaa
     8521 gacttcatta aacggcaaga aattaaaaaa taataaataa taactaatag tcgtccatag
     8581 gaaattggat tgacttaagt ccaacataat aaggaaaaaa acttatgagt ctcgcattta
     8641 ccaaaacgcg aagtactatt ggtattgtcg cacaaccagt atctgtggaa gtccatttat
     8701 caaatggatt gccaagtttt actatggtag gacttgctga aacagcagtg aaagaaagta
     8761 aagaccgcgt gcgcagtgcc attataaata gccaattcga atttccatgc cgtaaaatta
     8821 ccgttaatct tggccctgcc aatttaccta aaacaggaag tggctttgac ttgcctattg
     8881 cactagggat actcgcagca tcggaacaaa ttcctctcac caacctggca aaccacgaat
     8941 ttattggaga attggcttta agcggcgagc ttagaggggt ttctgccatt atacctgctg
     9001 ttcttgctgc tcataaagac aatcaacatc tgattatagc gaatgccaat gctgccgaag
     9061 cttctttaac cggccatcca aaagtcttta ctgccaataa cttacgcgaa gtttgtgatt
     9121 atctatgcca gggtacttct ttacagaact tacctcccaa accagatctt ttatttaata
     9181 accatgaatt ggattggtct gatattaagg ggcaacatca tgccaaaaat gctatggtca
     9241 ttgctgcctg tgggggacac agcattctgc taagtggcgc gccgggcagt ggaaaaacga
     9301 tgatggcaaa aagatttagt accttgctgc ccgagctatc cgaaacacaa gctctcgaat
     9361 gcgcagccat taattcaatt cgaggcaaat tacctgattt cagggaatgg cgtttgcctc
     9421 cttttcgtgc accacatcat actgcctcac ctgtcgcgct agtcggagga ggcaacccac
     9481 cgaaacccgg tgaaatttca ttagcccatc acggcgtatt atttttagat gaattacctg
     9541 aatttaatcg gcaagtatta gagacgctta gagaaccttt agagtcaggg catatttgca
     9601 tttccagagc agctgcacaa atagagtttc ctgccaaatt tcaattaatt gcagcaatga
     9661 atccttgccc ttgtggccaa tggggtaatt cacaagccaa ttgcttatgt actcctgata
     9721 gaatcagccg ctatttggca aaactttctg ctccattatt agatagaatc gatatgcagg
     9781 ttacaattca cgcattatct caagaggagt taattaaacc caatacccgt ttagaaaagc
     9841 aaagtcctgc tattagagaa aaggtaacaa agatgcatga aatacaaatg gcacgacaag
     9901 atagtctcaa tgccaattta aattccaaaa catgcgaaat ggtttgcgaa ttgggatcag
     9961 aagaacaatt gtttctccga gaagtgatga gtaaactgaa actgtctgct cgtggttacc
    10021 atcgcttact aaaagtaagc aggacaattg ctgatatgag tgaatgtaat aaggtcatgc
    10081 taaaccatct gcaacaggcg ttatcctata aacaaatctt gcatttgcct aaatgaaatt
    10141 tcttctcaag tattggtgtg tataaaaaat caattccgta ttaatcatat acaccaaaat
    10201 tgtcctataa ttatacataa atataaatta cggggccatc atgagaagca tacgactctt
    10261 gtttccttgc attttattaa tgataccatg gcaatttgca acagctaata gacctactga
    10321 taatgcgcta gcgattataa aatcatgcta tgatgaatca gtaaacctca gcaatgagtt
    10381 tactccctgc gttaccaaaa aactcaaaac tatgcctaac gcagaggatt ttcaattgat
    10441 tatgaatttt aaaactgacg aaaaaggcac gaatgaacga ataccagtct ttatggtcga
    10501 taaaacaggg tatatgtatt actgtatagt aaccacagga aaaactttaa tcataaatgc
    10561 ttgtgccgga gaacagggaa aaccgctgac gcaaggtcaa atgttatcca tcgatctacc
    10621 tgaataattt tgcggtttcc tatctcttgc gaagaatggc taaccatgag atcttcgaat
    10681 atagataaaa tgcataatca agcagaaaaa attatattac tcgcgag
//
LOCUS       DBNCWK010000106        10721 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.106, whole genome shotgun sequence.
ACCESSION   DBNCWK010000106 DBNCWK010000000
VERSION     DBNCWK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.106"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            69..1367
                     /locus_tag="AC06IO_002232"
     CDS             69..1367
                     /locus_tag="AC06IO_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875408.1"
                     /translation="MAHYNVQSESLNKASVSLIMPWMVWGLGCLFYFYECLLQVSPSV
                     MSNELMRDFSVTSHTLGILSGIYFYSYAAMQLPGGVLMDYFGPQRLLTLATVVCAVST
                     IAFGMTDNFFMACVARLMIGFGSAFAAVGTMKLAANWFPAQKFALLTGLMVTIGMMGA
                     IGGETPLALLIDNFGWRESMIIMGSVGLLLATLLILIARDTPKNFSPAIHQHPVEEER
                     LIPSLLTLIKNKQLWLVATYGGLMYMATPVFCGLWGVPFLMNKMLIAKSTAANFISLV
                     FVGWAIASPLWGLFSNRIGLRKPPMYIGCVGAMICSVLFIFAPITSSITMEILLFAFG
                     IFSAGFLPAFSVAKELCNKKYVATGLSFMNMMNMVGIALAQPLIGYILDKMWQGQMMG
                     TVRVYPLEAYYTALSILPIGMLVALLILPKIKETYCQSVN"
     gene            1404..2747
                     /gene="miaB"
                     /locus_tag="AC06IO_002233"
     CDS             1404..2747
                     /gene="miaB"
                     /locus_tag="AC06IO_002233"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIO9875409.1"
                     /translation="MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVI
                     LLNTCSIREKAQEKVFSQLGQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIV
                     FGPQTLHRLPALLNERLEKNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFDDVLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADL
                     ALLIHYIAAIDGIGRIRFTTSHPLAFSENLINAYAEVPELANHLHLPVQSGSDRILSL
                     MKRGYTALEFKSKIRKLRKIRPDIRLSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSF
                     SFIYSPRPGTPAANLPDDTPMEVKKQRLQILQNRLLMNAARYSESMIGSKQKILVTGF
                     SKKSSQQLSGRTECNRVVNFDGPPHLIGQFIDVQISDALPNSLRGRLLEKEMQPA"
     gene            2747..3712
                     /gene="rluD"
                     /locus_tag="AC06IO_002234"
     CDS             2747..3712
                     /gene="rluD"
                     /locus_tag="AC06IO_002234"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HIO9875410.1"
                     /translation="MIEHIQQQLSVPRELHNQRVDRVIARLLPEYSRSLISNWIKTGA
                     VTLNNLPCKPKDKALGGDLITINVDHTMVNTDFNFCKPENIPIQIIYEDSEVIVVNKP
                     AGLVVHPGAGNKEHTLVNALLHHCPELQLLPRAGIIHRLDKDTTGLLIVAKNLISHTS
                     LIRQMQNREIKRHYLTLVHGYVISGGTIDTGFGRHPKNRLKMAVTDFGRQAVTHYNIN
                     KHYKDFTLLNVSLMTGRTHQIRVHMNYINHPVVGDQLYGGRMKFPPNADEAILSVLKQ
                     FKRQALHACSISFCHPVKNTEITLNALLPDDFELLLNTLDQHDEK"
     gene            3702..4427
                     /gene="pgeF"
                     /locus_tag="AC06IO_002235"
     CDS             3702..4427
                     /gene="pgeF"
                     /locus_tag="AC06IO_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIO9875411.1"
                     /translation="MKNRFANWPAPENITALSTTRLYGFSNGPYASNNLGLHVGDNQQ
                     HVLKNRQQLIELIDLPAEPIWLEQTHSTKCITVETEKTRDADAAITRLPKLPLVIMTA
                     DCLPILLCNRQGTEIAAIHAGWKGLYNGIIENTLKKLNSNRTDIIAWIGPAICKKCYE
                     VGEEVYLSFTNKYSLTRNAFEPVHSKWLADLPQIAEIVLNLEGVKSVFQSNLCTFELK
                     NEFYSYRRESQTGRIGTFIWINE"
     gene            4444..5820
                     /locus_tag="AC06IO_002236"
     CDS             4444..5820
                     /locus_tag="AC06IO_002236"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIO9875412.1"
                     /translation="MITRIKLFIASLLVFFIPVADAAQDLTNMPSLAPVLKNAMPAIV
                     NVAVQGYLPNNMASGNADDDDGENSKQPSRIPEKGRKFESIGSGVIIDPKNGIIITND
                     HVIRNANLITITLQDGRRLKARLIGGDSETDLAVLKIDAKNLKSLVIGDSDKLEVGDY
                     VVAIGNPFGLNSFGNSQSATFGIVSALKRSDLNIEGVENFIQTDAAINPGNSGGALVN
                     AKGELIGINTAIISPYGGNVGIGFAIPINMAKDVAQQIIKFGSIHRGLMGIFVQHLTP
                     ELAQSMGYAEDFQGALVSQVNENSPAQLAGLKSGDVIVQINDTKITQATQVKTTISLL
                     RAGSTAKIKILRDNKPLTLDVEVTDIKKHEQKLQSNNPFLYGLALRNFEQESPPHGNV
                     VGVQVVGASETSAGWRAGLRPGDIIISANKTPIKDIKSLQTVAHEKKKQLLVQVLRGA
                     GALYLLII"
     gene            6128..6204
                     /locus_tag="AC06IO_002237"
     tRNA            6128..6204
                     /locus_tag="AC06IO_002237"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6162..6164,aa:Met,seq:cat)
     gene            6413..7549
                     /locus_tag="AC06IO_002238"
     CDS             6413..7549
                     /locus_tag="AC06IO_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIO9875413.1"
                     /translation="MFRINLIRYVALLFIITLNLDCISVAFSSQVIYGTQNAARVTGK
                     SLKRIYFLQLGAFSQKSNAQQLARKVQAKTQYPVIISHSGNAFSVRVGPVNTQELHQL
                     SIKLSQNSNLALINHHSSPKQALNIKSKPQIPVKETSRPLSTHSVAKEMVDKKGSWYV
                     AAKIGGQETTPDDQFTVNNDSGFASPFDKDIYTNSSDSSLVLGIGGGYRFTNMILPAI
                     SVGIYYSHFFNMDINGQVIQYSLPEFTNYNYQWKVKSDLVLASAKANVISWKNIMPFV
                     QGGVGVSSNKGSQYYENALPDVTPRISPNFANHTTTVIAYQLGAGLDWEIKPQWLLSL
                     GYEYSDLGKVRSGYGTDSWSRETLSSKNLTSNAGTLGFTYLFHG"
     gene            7632..10064
                     /gene="legT"
                     /locus_tag="AC06IO_002239"
     CDS             7632..10064
                     /gene="legT"
                     /locus_tag="AC06IO_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegT"
                     /protein_id="HIO9875414.1"
                     /translation="MTDLIFIKIMGERTNMKYSTRIIITTILMLSALLAYAGKDPISW
                     RVSGVFPAQSSVGQTYTVTYTFTNQLPFRLAKALIIERSASPNTEFNFNDQCSGRLLN
                     SGESCTVVVALNPLVIGLKTLQLTIAGYSKDRVPLPQASTLSTGQSLSPIVSFVMDGF
                     PSQLATGSTASYKFTFTNNGAISATNLSVNVSQSLGAPNFTTTCTNTLTPNGGVCEVD
                     GTYTVTSGSPSVQQVIASLSYSGPSGSPVTAQTFTQVVNPAAPLVGTLVPPNYLPPLM
                     IASPPPYTVQFLFTASGTVDITAPGSIACDQGGNDCSVNITPVINSCTPQTLTNAACQ
                     ITATFTAPPAAPLTYVLTATVPYSVGGNPQAPATAITSGTVVAALPTERTVTLINECD
                     FDVWFSLNGSQLGSSPNCPTTPCPNGTSCNTSTNKCFWNNPTPINGIYLLAAPPGANT
                     NSVTIPVTNADPSIQWSGNISASTLCNGVTCQQAACGNAGGTTSCAPGIGFTQPATQA
                     EITMNLTTSDSYDVEVINGFHIPISMQPIYYQQGPTTIIATPNNYNCGVPGNDTADNG
                     FGACNWNAATVPVIDQVPGNGFYWVTGGGQSCSITSANPGCPLMTLCGLDSNFNQVCG
                     NFLGYWSADQVCGSSNVPAVVQSYFKCNQPLPTATTPFYPSGAVLSNLMLCSVPTGFT
                     GPRYNTCYNAYPSSSPTDIAQCCGCADWWNPVQTNNIVIQANPNTESCTQPGALQPQT
                     NAQWNSFVQPMIQWMKQACPSAYIYPFDDKTSGFTCTNNLSGQPNSTSYTIRFCPGGI
                     TGLPAGVNEGRG"
     gene            complement(10144..10458)
                     /locus_tag="AC06IO_002240"
     CDS             complement(10144..10458)
                     /locus_tag="AC06IO_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HIO9875415.1"
                     /translation="MVIYEVNLTVDPEIFVQFQAWLKKHVAEIIQFPGFMKAYILKQE
                     KETKSGKEKLTVQYQLDNRDNLTRYLSELAPKMREEGIHLFQAKFFADRRIFEVQEII
                     SK"
BASE COUNT         3277 a         2181 c         2095 g         3168 t
ORIGIN      
        1 atcctctttt ttgactaata actttcatag aatctaatga ttggataaat ttggagaact
       61 tttaaataat ggctcattac aatgtacaat ccgaatcgct caataaagca agtgtcagct
      121 taattatgcc ctggatggtg tggggcttgg gttgcttatt ctatttttat gaatgcttat
      181 tacaggtatc cccaagcgta atgagtaatg aactcatgag agatttctcg gtaactagtc
      241 atactttagg tattctgtcg ggcatttatt tctactctta tgctgccatg cagcttcctg
      301 gtggtgttct aatggattat tttggcccac aacgtttatt aactcttgcc acagtagttt
      361 gtgccgtgag tactattgcc tttggcatga cagataattt ttttatggcc tgtgtcgcaa
      421 ggctgatgat tggatttggt tctgcatttg ctgcggttgg caccatgaaa ttggcagcca
      481 actggtttcc agcccaaaaa ttcgcattat tgacaggttt gatggtcacc atagggatga
      541 tgggtgcaat aggcggagaa acacctttgg cacttctaat tgataatttt ggctggcgtg
      601 aaagcatgat tatcatgggc agtgtagggc ttcttttagc gactttgctc atcttgattg
      661 ccagagatac tcccaagaat ttttctcccg ctatccatca acatcctgtc gaagaagaac
      721 gcttaatccc cagtctactc acactgataa agaataaaca actttggctg gttgctacct
      781 atggtgggct aatgtacatg gcaacacccg tattttgcgg gctttgggga gttcctttcc
      841 tgatgaataa aatgctaatt gcgaaatcta ccgccgctaa ttttatctca ttagtatttg
      901 tgggttgggc aatagccagt cctttatggg gactgttttc aaaccgtatt ggattacgta
      961 agccacctat gtatattgga tgtgtagggg caatgatttg ttctgtgctc tttatttttg
     1021 cccccatcac ttcttcaatt actatggaaa tcctgctgtt tgcatttggt atcttttccg
     1081 caggcttttt gccagctttc tcagtagcca aagagttatg taataaaaaa tatgtggcca
     1141 cagggcttag ttttatgaat atgatgaata tggttggaat agcgttagca caacctttaa
     1201 ttggttatat tctggataaa atgtggcaag gccaaatgat ggggactgta agagtttatc
     1261 ctttagaggc ttactacaca gctttgtcaa ttcttccaat aggtatgtta gttgcgctgc
     1321 tgatattgcc aaaaatcaaa gaaacctatt gccaaagtgt caattaatga aataataccg
     1381 caatttcaac ttgtagaacg aaaatggtta agaaattata cattaaaaca aatggctgcc
     1441 aaatgaatga atacgattct tctaaaatgg cagaagtact ttatgcttcc cacggtttgg
     1501 tcaaaactga tcaagtcgaa gatgccgatg ttattttatt aaacacatgc tcaattcgag
     1561 aaaaagcaca ggaaaaagtt ttttctcaac tgggtcaatg gcgggaatat aaagctaaaa
     1621 accctcatgt actcattggc gtgggaggat gtgtagcaag tcaggaaggt tcagatatca
     1681 taaaaagagc accgtttgtt gatatcgtat ttgggccaca aacattgcac aggctccctg
     1741 ccctgctcaa tgaaaggctt gaaaaaaata aatccgttgt tgatatcagc tttccagaaa
     1801 tagagaaatt tgatcattta cccgcaccaa gagcagaagg ccctacagct tttgtatcta
     1861 taatggaagg ctgtagtaaa tattgcagct tctgtgtagt gccctacacg cgtggcgaag
     1921 aaatcagcag accatttgat gatgtattgg ctgagtgtta tcaattagcc agtcaaggcg
     1981 tcagagagat taatttacta ggccaaaatg tgaacgatta cagaggtatt atggataatg
     2041 gggatatagc cgatcttgcc ctgttaattc attatattgc cgctattgat ggaataggaa
     2101 gaatacgttt cacaacatca catcccttgg cattctctga aaatttgatt aatgcctacg
     2161 ctgaggttcc agagcttgct aatcatttac accttccggt acaaagcggg tccgatcgca
     2221 ttttatcgtt aatgaaacga gggtacactg ctttggaatt taaatccaaa attcgtaaat
     2281 taaggaaaat tcgaccagac attcgcttat ctactgacat tattgtaggc tttcccggag
     2341 agacagacaa agatttccag gacactatgg atcttgtgca tgaaatcggt tttgatacct
     2401 ctttcagttt tatatacagc ccaagaccag gaactcctgc agcaaattta cctgatgaca
     2461 cgcctatgga agtaaaaaag cagcgtttac agattttaca aaacagatta ttaatgaatg
     2521 cagctcgtta cagtgaatca atgataggca gcaagcaaaa aattctggtt actggatttt
     2581 ccaagaaaag ttcacagcaa ttatctggtc gtactgaatg taaccgtgta gttaattttg
     2641 atggaccacc tcatttaatt ggacaattta tagatgttca aattagtgat gcactaccta
     2701 attcattgcg aggaagactg cttgaaaagg agatgcagcc agcctaatga ttgagcatat
     2761 ccaacaacaa ttatcagtac ctcgcgaatt acataaccaa cgagtagata gagtgattgc
     2821 caggctactt cctgagtatt cgcgctctct tatcagtaac tggattaaaa caggtgctgt
     2881 tacccttaat aatctgcctt gcaaacccaa agataaagcg ctgggcggtg atttaattac
     2941 gattaatgtt gaccatacca tggttaatac agattttaac ttttgtaaac ctgagaacat
     3001 tccaattcag ataatctatg aagatagtga agtaattgtt gttaacaagc cagccggtct
     3061 tgttgtacat ccgggtgcgg gaaacaagga acatacacta gttaatgcgc tgcttcatca
     3121 ttgtcccgag ttgcaactac ttcctcgtgc tggaattatt cacagattgg ataaagatac
     3181 cactggtctg ctgatagtgg ctaaaaacct gattagccat acttctctca tcaggcaaat
     3241 gcaaaatcgc gaaattaagc gccactatct gacattagtt catggttatg tcatttctgg
     3301 tggaacaata gatacgggtt ttggcaggca tcccaaaaat agactaaaga tggctgtaac
     3361 tgactttggg cgtcaagctg ttacccatta taatatcaat aagcattata aagactttac
     3421 cttactgaat gttagcttaa tgacaggacg gactcatcag ataagagtgc atatgaacta
     3481 cattaaccac ccggttgtag gagatcaact gtatggagga agaatgaagt ttcctcctaa
     3541 tgctgatgag gcaatactct cagttttaaa gcaatttaaa agacaagcac ttcatgcctg
     3601 ttccatttca ttttgtcatc ctgtaaaaaa tacagaaata acactgaatg cattattacc
     3661 cgatgatttc gaactcttgt taaacactct ggatcaacac gatgaaaaat agatttgcca
     3721 attggccggc tcctgaaaat attactgctt tgagtactac ccgtttgtat ggtttcagta
     3781 acggacccta tgcgagcaac aaccttgggt tgcatgttgg tgataaccaa cagcatgtac
     3841 tcaaaaacag acaacagctc attgaactta ttgatctgcc tgcagaacca atctggctgg
     3901 aacaaacaca tagcacaaaa tgcatcacgg tagaaactga aaaaaccaga gatgctgatg
     3961 ctgcaattac tcgtttacct aaactccctt tagtcattat gacagcagat tgcctaccca
     4021 tattgctatg caatcgacag ggaactgaaa ttgccgccat tcatgctgga tggaaaggcc
     4081 tgtataatgg tattattgaa aatacactaa agaagctcaa tagcaatagg acagatataa
     4141 ttgcctggat aggccctgct atctgtaaaa aatgttatga agtaggtgaa gaagtttatc
     4201 tgtcttttac caataaatac tccttaacca ggaatgcgtt tgagcctgtt catagcaaat
     4261 ggttagctga tttacctcaa attgcagaaa tagtacttaa tttggaaggg gtaaaatcag
     4321 tatttcagtc aaatttgtgt acttttgagt taaaaaatga attctattcc tatcgcagag
     4381 aatcacaaac aggtagaata ggtactttca tctggattaa tgagtaaaat caggattaat
     4441 aatatgatta cacgtataaa attattcata gcaagtctgc ttgtattttt catcccggta
     4501 gccgatgcag cacaagatct tacaaacatg cccagcttgg ctcctgttct gaaaaatgct
     4561 atgccggcta ttgttaacgt ggctgttcaa gggtacttgc ctaataatat ggcatccgga
     4621 aatgcagacg atgatgatgg ggaaaacagt aagcagcctt ccaggatacc tgaaaaaggc
     4681 agaaaatttg aaagtatagg gtccggagtt atcattgacc ctaaaaatgg cattattata
     4741 acgaatgacc atgttattcg taacgccaat ttaattacca taactcttca agatggcagg
     4801 agattaaaag ctcgcctgat tggaggcgat agcgaaacgg acttggctgt tttaaaaatt
     4861 gatgctaaaa atttgaaatc cctggtaatt ggtgattccg ataagctgga agtcggagat
     4921 tatgttgttg ctattggaaa cccctttgga ttaaacagct ttggaaatag tcaatctgct
     4981 acgtttggaa tagtgagtgc cttaaaacgc agcgatttaa atattgaagg tgttgaaaac
     5041 tttattcaaa ctgacgcagc tatcaatccc ggtaattcag gaggtgcttt ggtcaatgca
     5101 aaaggcgaat taattggcat taatacagcc attatttcac cctatggagg aaatgtaggt
     5161 attggctttg cgatcccaat taatatggca aaagatgtag cgcagcaaat cattaaattt
     5221 ggctctattc atcgtggttt aatgggtata tttgttcaac atttaacacc agaacttgct
     5281 caatcaatgg gatatgccga agattttcaa ggagctttag tatcacaggt caatgaaaat
     5341 tcacctgctc aattggctgg tctaaaatca ggcgatgtca ttgtgcaaat taatgacacc
     5401 aagataactc aggcaacaca ggtaaaaaca actattagcc tgttgcgagc cggctctact
     5461 gctaaaatta aaattttgcg ggataataag ccgcttacat tagatgtaga agtcacagat
     5521 atcaaaaaac atgaacaaaa attacaatcc aataatccat ttctctacgg attagccttg
     5581 cgtaattttg aacaagaatc accgcctcat ggtaatgttg ttggagttca ggttgtaggt
     5641 gcttcggaaa ccagtgcggg ctggcgagct ggcttaagac cgggggatat tatcatttct
     5701 gctaataaaa caccgattaa agacattaaa tcgttacaaa ccgttgcaca cgagaaaaag
     5761 aaacagttat tagtacaggt attaagagga gctggggcac tctacctgct gatcatttaa
     5821 atttcttatc ctaataacga aataacgcat cttctttatt taagcccatc tctgagagat
     5881 gggcttaaat tttgataaat tagcctgctt ttgataatgc tgactatcaa ctattccccc
     5941 tatcctccaa aacaatccct tgcttggggg aatatcactt tttaatatat caacaaactt
     6001 ctttttaaat acgctattct gctttgaaaa aataatgcaa atccaattta atataaatta
     6061 attataaaaa actcaggaaa cagtcttgac ataaacacat gaaaaagaca aaatcacgac
     6121 ctcttgtggc tatgtagctc agccggttag agcgcggcac tcataatgct gaggtcggtg
     6181 gttcgagtcc acccatagcc accatttcac atatgccctg attgaaaaca cttgaagtga
     6241 gcaatcaaac cctagttctg attttgtctt tcttttatca cttcaagcaa tagaatatcc
     6301 actgtaatca aatcagtccc ttcgccgaaa taatatttga gatcatcttg catcacgaaa
     6361 agaaattata gtagcatcgt tgctcatcac atttcataat acggatttta ttttgttcag
     6421 gattaactta atacgatatg tggcacttct gtttattatt acattgaatt tagattgtat
     6481 tagcgttgca ttttcatctc aggtaattta tggaacacaa aatgctgcac gagttactgg
     6541 aaaaagtctg aaaagaattt attttcttca gttaggcgct tttagccaaa aaagcaatgc
     6601 tcagcaattg gcaagaaaag ttcaagcgaa aacacaatac cctgtaataa ttagtcattc
     6661 aggcaatgct ttctcggttc gcgtggggcc tgtcaatacg caagaactgc atcaactcag
     6721 cataaaactt tcacaaaatt caaatcttgc tctgataaac catcactcat caccaaagca
     6781 ggcacttaat attaagtcaa aaccgcaaat ccctgttaag gaaacttctc gccctctttc
     6841 aactcattca gtagctaaag aaatggtgga taagaaagga agttggtatg tagctgctaa
     6901 aattggagga caagaaacaa cgccggatga tcagtttacc gtaaataatg acagtggttt
     6961 tgccagccct tttgataaag atatttatac caacagttcc gattcctcct tagtcttagg
     7021 tattggcgga ggctatcgat ttacaaatat gattctccct gcgatttcag ttggaatata
     7081 ctattcccat ttctttaata tggacattaa tggacaggtg attcagtaca gcttgcccga
     7141 attcaccaat tataattatc aatggaaagt aaaatctgat ttggttttag cttccgcaaa
     7201 agccaatgtc attagttgga aaaacataat gccgtttgtg caaggaggcg ttggagtgag
     7261 ctccaataaa ggaagtcaat attatgagaa tgccttgcct gatgtcacac ctcgtatcag
     7321 tcctaatttt gccaatcata cgactactgt gattgcttac caactgggag ctggtctgga
     7381 ttgggaaata aagcctcaat ggctgctttc cttgggatac gaatattccg atttaggaaa
     7441 agtacgttct ggttatggta ctgatagctg gagtagagag acattgtcct cgaaaaattt
     7501 gacaagcaat gcaggaacat taggctttac ctatttgttt catggttaat caaatatgag
     7561 gcacaagcta aaactccaaa tattgagaaa ccagaaatta cctttggctt tccagcggta
     7621 atttatccaa gatgactgat ttaatcttca taaaaataat gggtgaaagg accaacatga
     7681 aatactcaac tcgcatcata atcaccacaa ttcttatgtt atctgccttg ttggcttacg
     7741 caggaaaaga tcccataagc tggcgagtca gcggggtatt tcctgctcaa agcagcgttg
     7801 gtcagactta tactgtgact tatactttta ccaatcaatt gccttttcga ttggccaagg
     7861 ctttgattat agaacgatcg gcaagcccca acacggaatt caattttaat gatcaatgtt
     7921 ccggtcggtt attaaattcc ggagaatcct gtacggtagt tgtggcgtta aacccccttg
     7981 taattggtct taaaacactt caattgacta ttgcaggata cagcaaggat agagtacctt
     8041 tacctcaagc aagcacctta agcacaggac agtccctgag tccaattgtt agctttgtca
     8101 tggatggctt tccttctcaa ttagcgacag gctccactgc cagctataaa tttaccttta
     8161 ccaataacgg ggctatttca gctaccaatt tatcagttaa tgtctcgcaa tcattagggg
     8221 caccgaattt tactacgacg tgcactaaca cattgactcc caacggaggg gtttgtgaag
     8281 tcgatggcac atacacggta acctctggaa gtccatcagt tcaacaagtt atagcaagtc
     8341 tatcctattc aggcccatca ggaagcccgg taacagcaca aacattcacg caagtagtca
     8401 atccagcagc tccgcttgta ggcactttag taccgccaaa ttatctaccg ccattaatga
     8461 ttgcttcccc ccccccgtac acagtacagt ttttattcac cgccagtgga actgtagata
     8521 ttacagctcc tggctcaata gcctgtgacc agggaggtaa tgattgctcg gtaaatataa
     8581 ctcctgtcat caatagctgc accccgcaaa cattgaccaa cgcggcctgt caaattactg
     8641 ctacgttcac ggcacccccc gctgccccac ttacttacgt tctaaccgca acagttccct
     8701 attccgttgg aggaaatcct caagcaccag cgactgccat cacttccggg acagtagttg
     8761 ctgcattacc gacagaaaga actgtgactt taatcaatga gtgcgatttt gatgtgtggt
     8821 tttcgctgaa tggaagtcaa ttaggtagct cacccaattg cccaacaaca ccatgcccaa
     8881 atggcacaag ctgtaatacc agtacgaaca aatgcttttg gaacaatcca acaccaatta
     8941 atggtatata tttattagca gcccctcctg gcgctaatac taatagcgta actatccctg
     9001 ttaccaatgc tgatccaagt attcaatgga gtggaaatat ttcggcaagc actctgtgca
     9061 atggtgtaac ctgccaacaa gcggcttgtg gtaatgctgg cggtaccacc tcctgtgcgc
     9121 caggcattgg tttcacacaa cctgccactc aggccgaaat cacaatgaat ctaactacct
     9181 ccgacagtta tgatgtggaa gtcatcaatg gttttcatat cccaatttcc atgcaaccca
     9241 tttattatca acaaggaccc actactatta ttgctacccc gaataattac aattgcggag
     9301 tgcccggtaa cgatacggcc gataatggat ttggagcttg taactggaat gcagccactg
     9361 taccagtcat tgatcaagtg ccggggaatg gattttactg ggtaactggt ggcggtcaaa
     9421 gctgttccat cacctctgca aatccaggtt gccctttgat gacattatgc ggattagaca
     9481 gtaactttaa tcaggtttgt ggcaattttt taggatattg gtcagccgat caagtttgcg
     9541 gttcaagtaa tgtcccagcc gtggtacaaa gttattttaa atgcaatcaa cctcttccta
     9601 ctgctacaac ccctttttat ccttcaggcg ctgtcttgtc caatttgatg ctttgctctg
     9661 ttcccacagg atttactggc ccaagataca atacctgtta taatgcttat ccatcttctt
     9721 cccctacaga cattgcccaa tgttgtggat gcgcagactg gtggaatcct gtgcaaacca
     9781 acaatatagt aattcaagct aacccaaata cagaaagttg tactcagcct ggcgctttac
     9841 aaccacaaac caatgcccag tggaattcat ttgttcaacc aatgatacaa tggatgaaac
     9901 aagcttgccc ttctgcttat atttatcctt ttgacgacaa aaccagtgga tttacttgta
     9961 ctaataacct ttcagggcaa cccaactcta caagctatac tatcaggttc tgtccaggag
    10021 gcatcacagg tcttcctgca ggagtaaatg aggggcgtgg ataaaataca tggctggtat
    10081 taacgcttcg tactttatag ttgtaagaag cgagtcgtta ataccggtaa acatgaacaa
    10141 caattacttt gatataattt cctgcacttc aaagatgcgt ctgtcagcaa aaaatttagc
    10201 ttgaaacaaa tgaatacctt cctcacgcat cttaggtgcc agctcagaca agtaccttgt
    10261 caaattatct ctattgtcca gctggtattg aacagtcaat ttttccttac ccgattttgt
    10321 ttctttttcc tgctttagaa tgtaggcttt cataaatcct ggaaattgga ttatttccgc
    10381 aacatgtttt ttgagccatg cttgaaattg tacaaatatc tccggatcaa ccgttaggtt
    10441 tacttcataa atcaccataa tttatcttcc caaattccca tgatttgaat tcaccaattg
    10501 cacacaataa ctggatttat ttttagaata atgagtcatg ccatgattgg atacgatcta
    10561 ccatagttac ttgaatttta aatgaccgat tatctgtttg ccgctgattt acaatagtat
    10621 ccataaagca tactcagcat accttgaaca aatcaaaaaa tgtagactat ttggcaagac
    10681 attcaaaaat cagataacga tattaataac gcgatattcc c
//
LOCUS       DBNCWK010000107        10358 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.107, whole genome shotgun sequence.
ACCESSION   DBNCWK010000107 DBNCWK010000000
VERSION     DBNCWK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.107"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..436
                     /locus_tag="AC06IO_002241"
     CDS             <1..436
                     /locus_tag="AC06IO_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HIO9875416.1"
                     /translation="RDYEEGLYSQPSSVQDHWHAQLYFLKLPLRLGISFVWIWTALCC
                     LFFYPKSDSLSLFAQIGFSSLWQLFLFYASALWDGLLGIAMLFNYRFKLTSILQLITI
                     LVYTLIITIKLPYLWLDPFAPIAKNIPLLIAILIGLCLESDK"
     gene            436..903
                     /locus_tag="AC06IO_002242"
     CDS             436..903
                     /locus_tag="AC06IO_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIO9875417.1"
                     /translation="MLYFYLKFIHVLSSTILFGTGIGTASVMIYGHRTKNPIVIAAIS
                     KYVVFADWIFTGTSGILQPLTGFAMIYLAGFSWTSLWILGSILGYVVAACCWFPVVCL
                     QIKMRDLAIETVKNNTTLPEKYHRYFLYWFILGWPAFLSLVGVFYLMTNKPNF"
     gene            complement(957..3560)
                     /locus_tag="AC06IO_002243"
     CDS             complement(957..3560)
                     /locus_tag="AC06IO_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HIO9875418.1"
                     /translation="MKKTVLHAAIFSLTMPISLYALGLGEMKVQSSLDQPFSAEIELI
                     DVGADPIASIKVNLADPEQFEQIGLERTAVLSLLRFQVERNPKGTPVVKVSSTERMTE
                     PYMEIVVDLTWPKGQLYKAYTVLLDPPGYQLGITTAQSRPVYYKKKSTSYSNEPGVIE
                     KTVITTVEHGPVSRSDSKKKTTYGPTVTNENVWQIAQRYKTSKLILPQVVLAIVGANP
                     EAFKDGNLNGLKVGVRLVIPSTEEIAKVPSDLAIEEVMAHDKAWNEHTSINHVLAPPY
                     INSSIVDVTSKQTKASSIPVIPKFTIGEKSNIPTLQQLIPDSTSVSIDTSTQQQPVQN
                     NNIVQTTEQNEKIKAEISITTAAVESVWEANALLMEQLRILQEQNKKLQEKLDKREKE
                     IELMRAQVQTMLKERKAIASQVSSLPNNEQGMNIWPYIILLIFAAGGAGFAYWYLRQR
                     QHREDISPYKTDSDSSPKPFMPLVEPAKYEPDTQADDKETSVQEKVSDSEKETGIGVS
                     KADWTEYQPEEMDKIEKADAVESETVEDVTYPGGTEQLRAKEYTELESEVQAKPEVQA
                     KPEVQAKPEVQAKPEVQAKPEVKAKPEVQAKPEVQAKPEDQTKPEDQTKPEDQAEPED
                     QAGENQIMFEPVMEPEQLDVNRDDEVDNSQLSEDETQQTEGHQSSEMIEFESGLHELI
                     KDKPKTKITESTDEGDGNGIDFIPLTINDAEKTLKDEQEIEKSDKKEDKKSEEYSTKE
                     NKLDLDNSDFDMKNLESEPEISQEEKEGHISEITEFDQKSTQEEKTEKGISTSQSVDK
                     ATFTKSSGENNETSNPLKSKKALDTLLDLAKTYISMGDIETARSSLEEVIEHGSANQK
                     EQARQLLDQLK"
     gene            3830..4030
                     /locus_tag="AC06IO_002244"
     CDS             3830..4030
                     /locus_tag="AC06IO_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875419.1"
                     /translation="MNRENEVIEIFLMDISKKEKCKLLQDFLLDCKNEMEAQDQNMHP
                     EVHHNLSQAYQLAQNYLRKLQE"
     gene            4117..4971
                     /gene="folD"
                     /locus_tag="AC06IO_002245"
     CDS             4117..4971
                     /gene="folD"
                     /locus_tag="AC06IO_002245"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIO9875420.1"
                     /translation="MPASLIDGREISALRRAELKQRVQYHVEQGQRAPGLAVVLIGND
                     PASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLIELINELNQSDKIDGILIQLPLP
                     KHINERTIIEHIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
                     HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIAT
                     DWLREHQVVIDVGMHRLPDGSIRGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTM
                     MSAARLRE"
     gene            complement(4974..5183)
                     /locus_tag="AC06IO_002246"
     CDS             complement(4974..5183)
                     /locus_tag="AC06IO_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA3496 family putative envelope integrity
                     protein"
                     /protein_id="HIO9875421.1"
                     /translation="MGLHEEFEDQSFSDYNYDEEIDDHVDDLNQKKKIRRMLEDRLER
                     KRLKEEFKDDFDELSGEFDWDELDK"
     gene            5471..6319
                     /locus_tag="AC06IO_002247"
     CDS             5471..6319
                     /locus_tag="AC06IO_002247"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HIO9875422.1"
                     /translation="MLSYQHGYHAGNFADVIKHITLTRLLAYLTHKDKPLFYLETHSG
                     RGIYDLKDKQSLKTEEYKAGINPVWLDRENLPSLFLEYISVIKQINLNSTLRYYPGSP
                     YFAINQLRSQDRLYLCELHPTEYNFLLKLPHFNKKVYVNHTDGVSKLNALLPPPEKRG
                     LIFIDPSYERKEEYKEIPYAIKNAYSKFSTGLYCVWYPVVNKAWTEQFLRKMREISSK
                     SVRIELHLNPLINEGMTGCGLWIINPPYTFPSEIKAVLETLTTYFNPGSSSYMIESGS
                     KLCHEL"
     gene            6323..7087
                     /gene="gloB"
                     /locus_tag="AC06IO_002248"
     CDS             6323..7087
                     /gene="gloB"
                     /locus_tag="AC06IO_002248"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIO9875423.1"
                     /translation="MTILPISAFSDNYIWTFIDKIAGVLDCVDPGESAPIIRFAQSNQ
                     LTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPIDERISNLTHPIKQGQSVQVGPLH
                     FHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
                     NTKVFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFL
                     RTDKEQVKQYALSHGANSSDSLDVFEVLRNQKNSFK"
     gene            7201..8640
                     /locus_tag="AC06IO_002249"
     CDS             7201..8640
                     /locus_tag="AC06IO_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIO9875424.1"
                     /translation="MNCNLLIIKIFFSSILIFIGISNPAISGTAPDVWDVLRSEFTLS
                     HEVTRPEVQEQIRWLISHPSYINKVSSQSEPYIYHVLGEIKKRKLPGELALLPMIESA
                     YDPFAYSGAGAAGIWQLMPRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFS
                     GNWILAIAAYDSGEGTISRALKSTARNGRKVQFWNLPVPRETKVYIPRLLALAEIINN
                     PKRYKISLPNIPYLPYFEEVNIGSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYK
                     PFKLLIPAEKVERFNLNLSNIPEDKRVNWTKHQVKRGDSLDAIAKRYHTTVNLIKQLN
                     QLTSNKVQLNQSLLIPSSKNSPVAAKIPPQEKKPATKQPAPMKNVRIIHIVQNNDSYQ
                     KIQKAYGVAAKEIKNWNKLANNAPLRKGQRLVIWKRVKQPVVYIVKSGDSLSVIAKKH
                     QTKVNTIISLNPGLKKSMPLRLGQKILIG"
     gene            8884..9429
                     /locus_tag="AC06IO_002250"
     CDS             8884..9429
                     /locus_tag="AC06IO_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HIO9875425.1"
                     /translation="MKLLFDFFPIVLFFIVYKFFGIYTATAVAMVASLTQVAFYRLKF
                     QHYEKMHLFSLAIIMVLGGATLFFQNPWFIKWKPTGIYWLSALVFYGSGYIGSKPLIQ
                     KMMEANINLTTKIWYRLNLAWTLFFIVMGALNLYVAYHYDTDVWVNFKLFGGVGFTLL
                     FVLIQAFYLTKHTDEKSFEKQ"
     gene            9490..10167
                     /locus_tag="AC06IO_002251"
     CDS             9490..10167
                     /locus_tag="AC06IO_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIO9875426.1"
                     /translation="MRLLLVEDDELLGDAVKAGLTQFGYVVDWLKDGEAARAALKSES
                     FELIILDLGLPKLSGLSLLQNIRHDGNSTPVIILTARESIEDRVKGLDSGADDYLIKP
                     FDLNELSARIRALVRRSQGRADSVLQYKNITLDPAAHSVFVDDVLVNVPRREFALLQK
                     LLENSGQVLSREQLMQSIYGWEEDVDSNALEVHIHNLRKKLNANFIRTIRGVGYMAEK
                     NDSVLLS"
     gene            10164..>10358
                     /locus_tag="AC06IO_002252"
     CDS             10164..>10358
                     /locus_tag="AC06IO_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9875427.1"
                     /translation="MKSSIRKFLLINLLLAITITTTLTAIGNYYLDQKDIQDHLDTLM
                     AISALSYQALLGDDLHQRPLA"
BASE COUNT         3166 a         1947 c         1844 g         3401 t
ORIGIN      
        1 aagagactat gaagaaggtc tctatagtca accgagttca gtccaagacc attggcatgc
       61 gcaattatac tttcttaaac ttcccctaag actcggaata tcctttgttt ggatttggac
      121 agccttatgt tgtttgtttt tttaccctaa aagtgactca ttatctttgt ttgctcaaat
      181 aggtttttca tccttatggc agttattctt attttatgct tcagctttat gggatggcct
      241 tttaggtata gccatgttgt ttaattatcg atttaaattg accagtatat tgcaactgat
      301 tacgattttg gtatatacgc tgattattac catcaaactg ccttaccttt ggttagatcc
      361 atttgctccc atagccaaga acattccttt gttaatagct attttaatag gtctgtgttt
      421 ggagtctgat aaataatgct ctatttctat ttaaagttta tccacgtgtt gagttcaacg
      481 atactttttg gtacaggcat aggaacagca agtgtgatga tttatggcca tagaacaaaa
      541 aatccgattg ttattgctgc aatttcaaaa tatgttgttt ttgctgattg gatattcact
      601 ggtacatctg gaatattaca accactcaca gggtttgcca tgatttacct tgctggattt
      661 tcttggactt ctttatggat actgggatca attcttggat atgtcgttgc ggcatgttgt
      721 tggtttcctg tcgtctgcct gcaaatcaaa atgagggatt tagccataga gaccgtaaaa
      781 aataacacga ctttaccaga gaaatatcac cgatattttc tttattggtt tattctgggt
      841 tggcctgcat tcttaagcct tgttggagta ttttatttaa tgactaataa gcctaacttt
      901 taaatagcaa agatgaatat taagagattg ggatggcgta atttttgtgt tatttgttac
      961 tttaattgat ccagtaactg cctggcttgc tctttttgat tggcacttcc atgctcgatc
     1021 acttcctcaa gagaacttct tgctgtttct atatcgccca tactaatata ggtttttgcc
     1081 aaatctaaca aagtatctaa tgccttctta ctcttcaatg gattggaggt ttcattgttt
     1141 tcaccggatg acttggtgaa tgtcgcctta tcaacagatt gagatgtaga aatacctttt
     1201 tctgtttttt cttcctgagt agatttctgg tcaaattcag ttatttctga aatgtggcct
     1261 tctttttcct cctggcttat ttcaggttcg gattcgaggt ttttcatatc aaagtctgaa
     1321 ttatctaaat ctaacttatt ttcctttgta gaatattctt ctgatttttt atcttccttt
     1381 ttgtctgatt tctcgatttc ttgttcatcc tttaatgttt tttcagcatc atttatagtt
     1441 aaaggaatga aatctattcc attaccatct ccttcatctg ttgattctgt tattttagtt
     1501 ttgggcttat ctttgattaa ttcatgtaaa cccgactcaa actctatcat ctcagaactt
     1561 tgatggcctt cagtttgctg agtttcatct tcacttaact gagaattatc aacctcatca
     1621 tccctgttaa catccaattg ctcaggctcc ataacgggct caaacataat ttgattttct
     1681 ccagcctgat cttctggctc agcctgatct tctggtttag tctgatcttc tggtttagtc
     1741 tgatcttctg gtttagcctg aacctctggt ttagcctgaa cttctggttt agccttaact
     1801 tctggtttag cctgaacttc tggtttagcc tgaacttctg gtttagcctg aacttctggt
     1861 ttagcctgaa cttctggttt agcctgaact tctgattcta attctgtata ttcctttgct
     1921 cttaactgtt ctgtacctcc aggatatgtc acatcctcaa ctgtttcaga ttctactgca
     1981 tcagcttttt caattttatc catttcttca ggttgatact cagtccaatc cgctttagaa
     2041 acaccaattc cagtttcttt ttcactgtca gatacttttt cctggacgga agtttcctta
     2101 tcatcagcct gggtatctgg ttcgtatttc gctggctcaa ctaaaggcat aaatggcttc
     2161 ggacttgaat cagaatctgt tttgtaagga gaaatatcct ctctgtgttg gcgttgtctg
     2221 aggtaccaat aagcaaatcc tgctcctcct gcagcaaata tcaataatat gatatacggc
     2281 caaatgttca tgccttgttc attattggga agtgaactga cttgggacgc gatggctttg
     2341 cgctccttca acatcgtttg aacctgcgca cgcattaact ctatttcttt ctcacgctta
     2401 tccagctttt cctgcaattt tttattttgc tcttgcagaa ttcgtaattg ttccatcaat
     2461 aatgcatttg cttcccaaac tgattcaact gctgctgttg taattgaaat ttccgcctta
     2521 attttttcat tttgctccgt tgtctgtact atattgttgt tttgtacagg ttgctgttgg
     2581 gtagatgtat ctatcgaaac tgaagtgcta tcggggatta attgttgaag tgtcggaatg
     2641 ttagattttt ctccaatagt aaactttgga attaccggga ttgaagaagc tttagtctgt
     2701 tttgaagtca cgtccactat tgaactattg atgtatggcg gagctaatac atgattaata
     2761 gatgtatgtt cattccaggc tttatcatga gccatcacct cttcaatagc caaatcagaa
     2821 ggtacttttg caatttcttc agttgatgga ataactagcc gaacgccaac ttttaaaccg
     2881 tttaaatttc catccttaaa tgcttcggga ttcgcgccaa ctattgctaa tacaacctga
     2941 ggcaaaatca attttgaagt tttatatctt tgagcaattt gccaaacgtt ttcattggtt
     3001 acagttgggc cataagtcgt tttctttttg ctgtcacttc ttgatacagg gccatgctcc
     3061 actgtagtaa tcacagtctt ttcaataaca ccaggctcat tactgtaact tgtggatttc
     3121 ttcttgtaat aaactggcct actttgagcg gtggtaattc ccaattgata tcctggcggg
     3181 tctaataata ctgtataagc cttgtataat tgaccttttg gccaggttag atcgacgaca
     3241 atttccatat atggttctgt cattctttct gtagacgata cttttacaac aggagtacct
     3301 ttggggtttc tctcaacctg aaaccgtaac aaagacaaga ccgcagttcg ctcaagtcca
     3361 atttgttcaa attgctccgg atcagccaaa ttaaccttga tactggcaat aggatctgct
     3421 cctacatcta ttaattcgat ttcggctgaa aaaggctgat ccaatgagga ctgtaccttc
     3481 atttccccta agccaagcgc ataaagagat attggcatag tcaatgagaa tatagccgca
     3541 tgcagaacag tttttttcaa tcagagctcc atgcacctgt gatacaagat gttgatgatt
     3601 taattaccta agtaaaaagt atcattatcc tttagtctat tcaagaaaac acgaaattat
     3661 tccctaaaaa tcataagatg atgtataaaa ttacatcata tatttcatct aatttaacaa
     3721 gccaaatcaa ttgtggtctt ttaaaatcca accaggaaaa ttcataactc caatagacct
     3781 tcaactataa ttatatttaa ggattagtaa aattaaattt ggaatagcga tgaatagaga
     3841 aaatgaagtg atcgaaatat ttctaatgga tatcagcaaa aaggaaaaat gcaaattgtt
     3901 acaagatttt cttctggatt gcaaaaatga aatggaagcc caggatcaga acatgcatcc
     3961 tgaagtgcat cacaatttat cgcaagctta tcagctggcg caaaattatt taagaaaact
     4021 gcaagaataa gtgtattttc cttgttgcat atgaatcata ttgcattaga ataacccgta
     4081 attcataatg tactccataa aaattgcgag aagaaaatgc ctgcatcact aatcgatgga
     4141 agagaaatct ctgctcttcg cagagctgaa ctaaagcaaa gagtacaata ccatgtggag
     4201 caagggcaaa gggcgcctgg acttgctgtt gttttaattg gcaatgaccc tgcatcagtg
     4261 atttatgtca gtaacaaaag aaaagcttgt gaagaagtag ggatcacctc tcattcttac
     4321 gatttgcctg ccgaaacaac tcaggaaaaa ttaattgaac tgatcaatga gttaaatcaa
     4381 tcggataaaa ttgatggtat tttaatccaa ttacctttgc ccaaacatat taacgaaaga
     4441 actataatag aacacataaa gcccgagaaa gacgtggatg gatttcatcc ttataattta
     4501 ggccgacttg ctcaacgaaa cccattttta agaccatgca ctcccttggg gattatgaat
     4561 ttattacatc actatgagct caatgtcaaa agaaaacatg ccgtagtgat tggggcatcc
     4621 aatattgttg gaaggccaat gagtctggag ttactattag ctggtgctac cgtaacaata
     4681 tgccacaagt ttacccaaca attacagaag tttgttgaga ttgctgattt tctaatagta
     4741 gctactggaa agatggacgt cattgccact gactggctca gagagcatca agtagtgatt
     4801 gatgtaggaa tgcatcgttt gcctgatggc agtatcaggg gtgatattga ttttaagaag
     4861 gcagtcgaaa aagtagcctg gatcacacct gttccagggg gagttggccc aatgactatt
     4921 gtcactttgc ttgaaaatac tatgatgtca gcagcaagat taagagaatg aagttattta
     4981 tccagttcat cccaatcaaa ttcaccactt agctcatcaa aatcgtcttt gaactcttct
     5041 tttagacgct tacgttcaag cctatcttca agcatacgcc tgattttctt tttttgattt
     5101 aaatcatcta catgatcatc aatttcttcg tcgtagttgt aatctgaaaa tgattgatcc
     5161 tcaaactctt catgtaaacc catcttagtc tcccaattta ctcccctcaa aaaaagtata
     5221 gtgcataaat tatgaaataa agtaactgga agcaaaatag gcctcttgca taacctgatt
     5281 attctgcttc agggtatggc gcatatgttt tgagaggctg agttcacatg cagtacataa
     5341 gtgttgaaga aacgcttatg atgcgggagt aaaacaaaag agaccgctta agcaagaagt
     5401 ctaatatgcg gctacagcat aaaccattat tttgatataa tttggaaaat atcttttgaa
     5461 gccctctttc atgctcagtt atcaacatgg ctatcatgcc ggaaattttg ctgatgtcat
     5521 caagcacatc accctaactc gactattggc ttatctaacc cacaaagata aacccttatt
     5581 ctatcttgaa acccattcag gtcgagggat atatgattta aaagacaagc agtctttaaa
     5641 aacagaagaa tataaggcag gaattaatcc agtatggctc gatagagaga atctcccctc
     5701 tctttttctt gaatatatta gtgtaatcaa gcaaataaat cttaattcaa cattgcgtta
     5761 ttatcctggt tccccctatt tcgcaatcaa tcaactgcgc tctcaagacc gattatactt
     5821 atgtgaatta cacccaacag aatataattt cttattaaaa cttcctcatt ttaataaaaa
     5881 agtttatgtt aaccacacgg atggagtttc aaaattaaat gcccttttgc cgccaccgga
     5941 aaaacgtggg ttaattttta ttgatccctc atatgaaaga aaagaagaat acaaagaaat
     6001 tccatacgct ataaaaaatg cttactcaaa attctctacc ggcttatatt gtgtttggta
     6061 tcctgttgtc aataaagcat ggactgaaca attcttgagg aaaatgagag aaattagttc
     6121 caaatcggta aggattgagc ttcatttgaa tccactcatt aatgaaggaa tgactggttg
     6181 tggtctttgg ataattaatc ctccctatac tttcccttca gaaatcaagg cagttttaga
     6241 gacattaaca acttatttta accctggctc atcttcttat atgattgaat caggttcaaa
     6301 attatgtcac gagctataaa ttatgactat cctgccaatt tcggcttttt cagataatta
     6361 tatctggacc tttattgata aaatagctgg cgtattagat tgcgtcgatc cgggagagtc
     6421 ggcaccaata atacgctttg cccaatccaa ccaactgaca ctaagaacga tattattaac
     6481 ccatcaccac tatgatcata taggaggtgt tgactcgcta atcaaacaat ggccatcttg
     6541 taaggtctat ggccccatag atgaacgaat cagtaattta acccacccaa ttaaacaagg
     6601 tcaatctgtt caagtgggcc ctcttcactt tcatatttta tttaatccag ggcatacttc
     6661 tacccatatc agttattatg aaccacaaca aggatggtta ttttgtggag atactctatt
     6721 ttctgctggt tgcggtagag tgtttgatgg aactatagag gaattgcatg aatccctgct
     6781 cttattcaaa aaactcccgc gaaatactaa agttttttgt gctcatgaat acaccttaca
     6841 gaatttaaaa tttgcacata ccgttgagcc ctgcaattca tcagtcatca attacatgca
     6901 acaaattctg aaacaaccat caccttgtac tctcccgtct aatatagacc tggaattatc
     6961 aattaatcct tttttacgta ctgataaaga gcaagtaaaa caatatgctc tctcgcatgg
     7021 tgcgaattct tcagattctc tggacgtttt tgaggtattg agaaatcaaa aaaactcgtt
     7081 caaataatac aacaaaggat caaataaaaa cccatttgca tccaattaaa ccaaaaaggg
     7141 tttaatcctt cattaatttt tagtaaactg attagccaat tataataaca ggtaagacca
     7201 ttgaattgta atttattgat tataaaaata tttttttcat ccatccttat ttttattggt
     7261 atttctaacc cggcaatttc tggcacagct ccagatgtat gggatgtatt acgtagtgaa
     7321 tttaccttaa gccatgaagt aactcgccca gaagtgcaag aacaaatcag atggctaatt
     7381 tctcatccca gttatatcaa caaagtaagt agccaatctg aaccttacat ttaccacgtg
     7441 ttaggtgaga taaagaaaag aaaactgccc ggagaattgg cgctactccc catgatagaa
     7501 agtgcatatg atccttttgc ctattcagga gcaggcgctg ctggtatatg gcaactcatg
     7561 cctcgaactg gaagcgatct tggattaaag caagactggt ggtttgatgg aagacgtagc
     7621 attggccctt caactgatgc cgcattaaat tacctcgtct atctaaataa atattttagt
     7681 ggtaactgga ttttagctat tgccgcttat gactctggag aaggaaccat ttcacgggcg
     7741 ctcaaatcaa ccgcacgtaa cgggcgcaag gtgcaattct ggaatctgcc agttcccaga
     7801 gaaaccaagg tatatatccc gcgtttatta gctctggcag aaattattaa caatccaaaa
     7861 cggtataaaa tatcacttcc caatattccc tatcttcctt attttgaaga agtcaatatt
     7921 ggtagtcaaa ttgatttaaa tcatgctgct aaactcgccg gcatcagtta taaggagttg
     7981 attaaactaa atcccggtta taatcgatgg acaacggcac cttataagcc atttaaatta
     8041 ctaatacctg ctgaaaaagt cgaaagattt aatttgaatc tatcgaatat acccgaagat
     8101 aagcgcgtaa actggactaa acatcaagta aaaagaggtg acagcttgga tgctattgca
     8161 aagagatatc acacaacagt caatttgata aaacaactca atcaattaac cagcaataaa
     8221 gttcaactta atcaatccct actcattccg agcagcaaaa attccccagt agcagctaaa
     8281 atacctccac aggaaaaaaa acctgctact aaacagccgg cacctatgaa aaatgtccgt
     8341 attattcata tcgtacaaaa taatgatagc tatcagaaaa ttcaaaaagc ttatggggtt
     8401 gcggctaaag agataaaaaa ctggaataaa ttggcaaata acgcgccatt acgtaaaggc
     8461 cagcggctgg tcatatggaa aagagtcaag caacctgttg tatatatagt aaaatcaggg
     8521 gatagtttga gcgttattgc caaaaagcac caaacaaaag tgaatactat tatcagttta
     8581 aatccaggac ttaaaaaatc aatgcccctt cgcttgggac aaaaaatatt aattggctag
     8641 attatgtgca ttatcatgat ataaactagg tagattaata tatctgtttg aaacagaaac
     8701 gtgcttttga gggaatacca agactactgg catgacgcta aaagcttaaa ggttattaaa
     8761 taaaaaatct gtgataagta tgtaaattgt gcaaattttt ttgttaatac ggtatgaagc
     8821 ttgcaggtga gttctaaaac ttgtgttata atgatattaa ttaattgata aattaaacaa
     8881 tatatgaaat tactattcga tttttttcct atcgttttat tttttatagt ctataaattt
     8941 tttggcattt ataccgctac tgctgttgct atggtagcct cattaactca agttgcgttt
     9001 tacagattaa agtttcagca ttatgaaaaa atgcatttgt ttagtctggc tatcattatg
     9061 gtcttgggag gagcgacgtt attctttcaa aacccttggt ttatcaaatg gaagcctaca
     9121 ggaatctact ggttatctgc acttgtcttt tatggttcag gttatatagg gtctaagcca
     9181 ctaatacaaa aaatgatgga agcaaacatc aatttaacaa caaaaatttg gtatcgactt
     9241 aatttggcat ggacattatt ttttatagta atgggggcat taaatttata tgttgcctat
     9301 cattatgaca ctgatgtatg ggttaatttt aaattatttg gtggtgttgg gtttacttta
     9361 ctttttgtgt tgattcaagc tttttattta acaaaacaca cagatgaaaa aagctttgaa
     9421 aaacaatagt gacatctctg tatctgcaga aaaatatagc atgtaataag agaaaaagta
     9481 ggagcaccga tgagattatt acttgttgaa gacgatgaac tgcttggcga tgcagtaaaa
     9541 gccggactga cacaatttgg ttatgttgtt gactggctaa aagatggcga agctgctcgg
     9601 gcggctctca aatcagagtc ttttgaatta attattcttg acttgggatt acctaaatta
     9661 tccggtttat cattattgca aaacatcaga catgatggaa atagcacacc tgtgattatt
     9721 cttacagctc gagaatcaat agaagatcgg gtgaaaggct tagatagcgg tgctgacgat
     9781 tatcttatta aaccttttga tttaaacgaa ttaagtgcaa ggattagagc attggtaaga
     9841 cgctctcagg gcagagctga ttccgtgttg caatataaaa atattaccct cgatcctgct
     9901 gctcattctg tattcgtgga tgatgtgctc gtcaatgttc caagaagaga atttgctcta
     9961 ttgcagaaat tacttgaaaa cagtggacag gttttatctc gagaacaatt aatgcagagt
    10021 atttatggct gggaagaaga cgtggacagt aatgctctcg aagttcatat ccataattta
    10081 cgtaaaaaac ttaacgcaaa ctttatccgc actatacgtg gtgtaggata tatggctgaa
    10141 aaaaatgaca gtgtacttct atcgtgaaat cgtccattcg aaaattttta ttaattaatc
    10201 tgcttcttgc aattactatt actacaacat taactgctat tggtaactat tatcttgatc
    10261 aaaaggacat tcaagaccac cttgatacat tgatggccat ttccgcacta tcctatcaag
    10321 ccttattagg ggatgatctt catcaaaggc ctcttgca
//
LOCUS       DBNCWK010000108        10355 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.108, whole genome shotgun sequence.
ACCESSION   DBNCWK010000108 DBNCWK010000000
VERSION     DBNCWK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.108"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(203..970)
                     /gene="pcsA"
                     /locus_tag="AC06IO_002253"
     CDS             complement(203..970)
                     /gene="pcsA"
                     /locus_tag="AC06IO_002253"
                     /EC_number="2.7.8.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050520 - phosphatidylcholine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine synthase"
                     /protein_id="HIO9875428.1"
                     /translation="MNPIKPPYTLNQYFAAWFVHVFTASAACIGVFSLYKIYQHDYVF
                     ALWLMAITVFIDAVDGSLARLVHVKSVLPKIDGALLDNIVDYLNYVITPCFFLLVKPG
                     MLPADYVVPIIAAITITSAYQFCQDDAKTPDHFFKGFPCYWNITVFYMYIFNTSMIVN
                     TVLLSLFCVLIFIPVKYVYPSRLDYLTESRILKILMHCCSALYGISSFCLLVNYPETN
                     KLWVSLSLGYVGMYLFLSFYRTYYPMFKAKITANNKD"
     gene            1302..2690
                     /locus_tag="AC06IO_002254"
     CDS             1302..2690
                     /locus_tag="AC06IO_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIO9875429.1"
                     /translation="MNIQTVNPTTEEVLNNYTCLDFDSIDKKIACGYEAYLNWKNSKF
                     DIRRSLMLNLAQILKDKKQELAHLMALEMGKPITAGRAEIEKCAWVCEHYAENAEQYL
                     MPRTVQTEMKTAKVCYRPLGIVFAIMPWNFPFWQVFRFSAPTIMAGNGAILKHAPISS
                     GTGNKIEELFRDAGFPSSLFQHLIVDNDGASHIIENQFVTAVTLTGSERAGQSVAASA
                     GKNLKKSVLELGGSDPYLILEDADLDLAAQCIVASRLNNTGQVCIAAKRVIALQSIEK
                     ELISKIKELISKYKMGDPLDPNINLGPMARKDLRENLHSQVEKSVKQGAKLLLGGIIP
                     EGKGYFYPPTLLTDVKPGMTAFDEELFGPVIVIITAKNESEAISLANMSQYGLGAAVF
                     TKDLNKGEHIATYEIEAGTCFVNAFVASDPRLPFGGIKRSGYGRELSREGILEFVNTK
                     TIAISDPKHGKK"
     gene            2677..3165
                     /locus_tag="AC06IO_002255"
     CDS             2677..3165
                     /locus_tag="AC06IO_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIO9875430.1"
                     /translation="MVKNSKFIFISIKKQEMSCFEKDHLIANYAISSGKNGVGEKINS
                     GCTPRGWHKIHSILGIDLPINSVMVAREWTGEIYSESLATQFPERDWILTRILQLDGL
                     EPGRNKGGDVDTLYRYIYIHGTPDTTQLGIPGSHGCIRLNNDAIIELAAWATKNTLVY
                     IQ"
     gene            3217..8094
                     /locus_tag="AC06IO_002256"
     CDS             3217..8094
                     /locus_tag="AC06IO_002256"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIO9875431.1"
                     /translation="MSYKFEEGKDAIVKSVVEKIKQTMTGEQTEFCAEFARQFYGTVA
                     LEDLLEWEVDDLYGAAVNFWSLICERAPHETKIRIYNPDYERHGWQTTHTVVEVICED
                     MPFIVDSLRIVINRMGLTSHLTIHMGGIRVKRDSHNRVTEILPRNGGAARDDVLHEAP
                     IFIEIDRQTDPATLSELHKNFERALEDNRAVFEDWDKMRTKVREIIKELDTVPKTIDI
                     SEIEETKAFLNWMEDHHFTFLGLRDYDLVKKGKETILQPIPETGLGVLRQSLSKSNAR
                     SITAMGPEAQGLISSPRILVMSKTNTLASVHRDAYTDYIGVKRFNKKGEVIGERRIIG
                     LYTSAAYHTNPKHIPFLRHKVALIMKNSNLNPYSHAGKVLLNILETLPRDDLIQGTED
                     ELLEIAMGIFYMQDRKRIRLFARADVYKRFISCLVYVPKDRFNTELRYAMQKILADSF
                     NAEEITFSTQFSESVLARIHFIVRVNPKNLPDFDLKEIEQKLIEVGRSWIDDLQHHLH
                     EVYGEEQANALYSRYKNAFPISYSDTFSPRTAVYDIKHIEMLSPENPLGINFYKPLDE
                     SEKSFRLKVYQHDTTIPLSDVLPILEKLGLRAISERPYVLKFEDGKVAWINDFAMQYN
                     KESEFNIDEIKELFQNAFARVWFGDAENDGFNLLVLAAGLNWREVAVLRTYAKYFRQI
                     GFTFSQDYMETALNNNVSIAKKLVRLFEIRCNPHDSKRREDRYVALVAEILSDLDNVA
                     NLDEDRIIRQYVHAIGATLRTNFYQVDAQGNPKNYISIKLSSKLIPGVPKPYPMFEIF
                     VYSPRFEGVHLRCGKVARGGLRWSDRREDFRTEILGLMKAQQVKNSVIVPSGAKGGFV
                     PKQIPANATREEIMEEGISCYKLFIRGLLDITDNYKEGLIVKPQNVVFYDEDDPYLVV
                     AADKGTATFSDIANSISQEYDFWLGDAFASGGSVGYDHKKMGITAKGAWESVKRHFYE
                     LNRDIQNNDFTVVGIGDMAGDVFGNGMLLSRHIKLVGAFNHIHIFVDPNPEAEASFKE
                     RERLFNLPRSNWTDYDKKLISKGGGIFSRSAKSIPVSPEMQKVFGIKQTSIEPNDLIK
                     AILKADVDLLWSAGIGTYVKSSTESNTSVGDRTNDATRVNANQLRCKVIGEGGNLGLT
                     QLARVEYSLHGGKVYTDFIDNSGGVNCSDKEVNIKILLNNVVSAGDLTPKQRNELLSN
                     MTDEVAKLVLRDNFLQTRAISLTASQALRAIELHARYINELEKTGKIDRTLEFLPDEK
                     VLMEHKLMGKGLTQPGIAVLMCYSKTILKEQVLASEVPEEDYMNQILIGSFPKPLQER
                     FSKQMQDHPLRREIIATRLSNIIVNEMGFTYVYRLQDETGASVAAIVRAYMIARSVLN
                     LEAIWKQIEELGTKISAQAQVDMMMLYVRLSRRVTRWFLRTHRRSMSITQAIELYAKG
                     VDELKKSMPAVFGETGRIQYEEHYQEWVKEGIPPQLAHELTVTRGLFAATDIIEIAYE
                     ENIKISKVAEIYFGIGEFLDIAWLRTQIIVHTTENHWESLSREALRDDLDWQQRQLTA
                     AIMGFEPNNKDLQERLTRWGETHVSLIDRWNYILTALKSSTALNYTMFLVATRELLDL
                     TQTTLQSNLKMEESF"
     gene            8115..8663
                     /locus_tag="AC06IO_002257"
     CDS             8115..8663
                     /locus_tag="AC06IO_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIO9875432.1"
                     /translation="MEAKTASEYSTLSKLFHWIIALAVIIMLIAGFFLDEIPEQFQGV
                     AYMLHKSTGITILFLMILRFIWIHAKGKPALPASVKSWEKILSRFVQYGFYALLIIMP
                     LSGWIMSVAADRIPNYFGLFKAPLPWIEPNKSLAKLMAESHEIIAWILIAFITLHVLG
                     ALKHHFIDKDNVLKRMLPGKSK"
     gene            complement(8694..9683)
                     /locus_tag="AC06IO_002258"
     CDS             complement(8694..9683)
                     /locus_tag="AC06IO_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005739 - mitochondrion [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgfZ/GcvT domain-containing protein"
                     /protein_id="HIO9875433.1"
                     /translation="MKTMLEHLVNNRSLITFRPIESELLLNKQKNYLFDLSYLGVIDL
                     FGEKSIDFLQGQLTCDLRLVSDVSMVQGAQCNLKGRILSLLDIINWQGVKLVLPQDLI
                     EVTQNSLNKVALLSRVKITSNNNYKILGFYLQHDNDQIPQLLPLPSELYALSCTTHGC
                     VYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLFL
                     PHRIGLHQTAYVSFDKGCYKGQEIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKA
                     DENTEVGELIDYSMLGENRYLVAASILKENFSSVCLEGNKFPIQLEGKIELEL"
     gene            9809..10048
                     /locus_tag="AC06IO_002259"
     CDS             9809..10048
                     /locus_tag="AC06IO_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HIO9875434.1"
                     /translation="MDNREKSRLLWKCRRGMLELDLVLQKFIANEIDRLTENQLKAFD
                     NLLTHNDPNLYAWLMGHEEPEKELLEIVSFIRNCD"
     gene            9990..>10355
                     /locus_tag="AC06IO_002260"
     CDS             9990..>10355
                     /locus_tag="AC06IO_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1578 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875435.1"
                     /translation="MRNPKKSCLKLYHSSETVIKPGKSKVYLRLIAILYLCTIGLVLY
                     SSLPVLIEGVLIFLIVIQFKNDVTYQSACQKVKEIKYLKDLTWSLETIDGTTHHYDKL
                     KILIHNMLFQVIQLSSDKKN"
BASE COUNT         3506 a         1669 c         2055 g         3125 t
ORIGIN      
        1 tcaggtattc ataagttatt ggcataattt aacttctata gaggcgtaga ttttatatca
       61 attgcttgca ttacctctct attttttcag taaacagata ctatttaaag caataagaac
      121 agaaaaattc ctaaaagagt aagataaaac agtgccgcca gaaatgtaat atctgcatgg
      181 aaaacataaa ttacatttaa actcaatctt tattatttgc cgtaatctta gctttgaaca
      241 ttggataata agtcctgtag aaacttaaaa ataaatacat gcccacgtag cctaaagata
      301 ggcttaccca taatttattt gtttcagggt aattaactag caggcaaaat gaactgatgc
      361 cataaagagc agaacaacaa tgcattaata ttttcaagat tctcgactca gtcaagtaat
      421 ctaaccttga tgggtaaaca tattttactg gaataaaaat cagtacacaa aaaagggaca
      481 agaggacagt gttcacaatc atagaagtgt taaaaatata catatagaag acagtaatat
      541 tccaatagca tgggaaccct ttaaaaaagt gatcaggagt tttggcgtca tcctggcaga
      601 attgataggc tgatgtaatt gtaattgcgg ctataattgg cacgacataa tcagctggca
      661 gcatacccgg cttaactagc aagaaaaaac atggagtaat gacataattc aaatagtcaa
      721 ctatattgtc taataaagcg ccatcaattt taggcaatac gctttttaca tgaaccaatc
      781 gagcaaggct accatctact gcgtcaataa atacagtaat tgccattagc cataaagcaa
      841 atacataatc atgctggtaa attttatata agctaaagac accaatacag gcagcgctgg
      901 cagtaaatac atggacgaac caggcggcaa aatattgatt aagggtatag ggaggcttta
      961 ttggattcat tacttatcct agagttaatc aatgtaatta gggcttcagt ctgttttgta
     1021 aaagcaaaaa acaagttacc tttaaaaata tccagcgcaa tgattaaatc tggattgttt
     1081 tgttcatcaa gacgatgtgt ttttgctttt tactaaaaca gtacaagtcc tttttactta
     1141 acaggttgat ttttattact gttctttaag acaatgtcgt ttaaaataaa aatagtatca
     1201 atattcaaaa gcaaagtgca agttaggtta attatttaaa ataaaaaaaa gccttgattt
     1261 ggtctaaact ataggcaacg ttaatcatat cgagtaatta aatgaatatt caaactgtca
     1321 acccgacaac tgaagaagtt ctaaataact acacatgctt agattttgat tcaatagaca
     1381 aaaaaatagc gtgcggatat gaggcctatt taaattggaa aaattctaaa ttcgacataa
     1441 ggcgctcttt aatgctcaat cttgctcaga tattaaaaga taaaaagcaa gagttagcgc
     1501 atttaatggc tttagaaatg ggtaaaccca ttacagcagg tcgtgctgaa attgagaagt
     1561 gtgcttgggt atgtgaacat tatgctgaaa atgcagaaca atacctaatg ccaagaactg
     1621 ttcaaactga aatgaaaaca gcaaaagttt gttacagacc actaggaatt gtatttgcca
     1681 taatgccttg gaatttccca ttttggcagg tttttagatt ttcagcacca actatcatgg
     1741 cgggtaatgg ggctatttta aaacatgctc caatatcgag tggaacagga aataaaatcg
     1801 aagaattatt cagagacgct ggatttcctt cttcattatt tcaacactta attgtagata
     1861 atgatggggc atctcatatc attgaaaatc aatttgttac cgcagttact ttaactggaa
     1921 gtgaacgtgc tggtcaaagt gttgcagcga gtgcaggtaa gaacttgaaa aaatcggttc
     1981 tagaattagg cggctcagac ccctatttga ttcttgaaga tgcggattta gatcttgctg
     2041 ctcaatgcat tgtggcttca cgattaaata ataccgggca ggtctgcatt gcggccaagc
     2101 gagttatagc acttcaatcg attgaaaaag aattaatttc taaaatcaaa gaactcatca
     2161 gcaaatacaa aatgggagat ccattagacc ccaatattaa tttgggaccc atggcaagaa
     2221 aagatttacg agaaaatttg cattcacaag tagaaaaatc tgttaaacaa ggtgctaaat
     2281 tattgcttgg tggtataatt ccggagggta aagggtactt ctacccgcca acattattaa
     2341 ctgatgttaa acctggaatg acagcttttg atgaggaact atttgggcct gttattgtta
     2401 ttattactgc taaaaatgaa agtgaggcaa tttcactggc caatatgagc cagtatggtt
     2461 taggtgctgc tgtatttaca aaagatctta ataagggaga gcatattgct acatatgaaa
     2521 ttgaagcagg aacctgtttt gttaatgctt ttgtagcctc tgatccaaga cttccttttg
     2581 ggggaataaa gcgatcggga tatggtagag agttatcaag agaaggtata ttagagtttg
     2641 taaataccaa aacaattgcg attagtgacc caaaacatgg taaaaaatag taaattcatt
     2701 tttatttcaa taaaaaaaca ggagatgagt tgttttgaaa aggatcattt aattgcaaac
     2761 tatgctattt catccgggaa aaatggcgtg ggtgaaaaaa ttaatagtgg atgcaccccg
     2821 agaggttggc ataagatcca ttctatactt ggcattgatc tgccaattaa tagtgtgatg
     2881 gttgccagag aatggactgg tgagatttat tcagaatcat tggcaactca atttccagaa
     2941 agggattgga tattaactcg aattttacaa ttggatggat tagagcctgg tcgaaacaag
     3001 ggcggagatg tagatacttt atatcgttac atttatattc acggcacacc agatacaact
     3061 caactaggta tacctggctc tcatggatgc attcgcctga ataatgatgc aattattgag
     3121 ctggcagctt gggcaacaaa gaatacttta gtatacatac aatgattaat aacaattaat
     3181 gcttcggagc ataagtgaac ctgaaggggt aaatgaatgt cctataaatt tgaagaaggt
     3241 aaagacgcaa tagtaaaaag cgttgttgaa aaaattaagc aaacaatgac tggcgaacaa
     3301 acagagtttt gcgctgaatt cgcgaggcaa ttttatggga ctgtcgccct tgaagatctt
     3361 ctagaatggg aggttgatga tctttatgga gcagcagtca atttctggtc tttgatttgt
     3421 gaacgcgctc cacacgaaac aaaaatcagg atttataatc ctgattatga acgtcatgga
     3481 tggcaaacta cacatactgt tgtagaagtt atctgcgaag atatgccttt tattgtagat
     3541 tctctgagga tagtaattaa tcgaatggga ttaacatctc atcttactat tcatatggga
     3601 ggaattaggg ttaaaagaga cagccataac agggttaccg aaattcttcc aagaaatggt
     3661 ggagccgcta gagatgatgt tttacatgag gcccccattt ttatagaaat tgatcgacaa
     3721 acagacccgg ctactttatc ggagctgcac aaaaattttg agcgcgctct tgaggacaat
     3781 cgagcggtat ttgaagattg ggataaaatg cgtactaaag tacgagaaat tattaaggag
     3841 cttgatactg taccaaagac aattgatatc agtgaaattg aagagacgaa agccttttta
     3901 aattggatgg aagatcacca ttttactttt ttaggattgc gagattatga tttggttaaa
     3961 aaaggaaagg aaaccatttt acagcctatt cctgaaacag gattgggcgt tttgcgccaa
     4021 agtctgagca aatcaaatgc aagaagtatt acggcaatgg gaccagaggc acaaggatta
     4081 atttcatcac cccgtattct tgtaatgtcc aaaacaaata ctttagcctc tgttcataga
     4141 gatgcttata ccgattatat tggtgttaag cgcttcaata aaaaaggaga ggtgattggc
     4201 gaaaggcgca ttataggact gtatacttct gcagcttatc atacaaatcc caagcatatt
     4261 ccgtttttgc gacataaagt cgcattaatt atgaaaaatt ctaatttaaa tccctatagc
     4321 catgcaggta aagtgttatt gaatatactt gaaactcttc caagagatga tttaattcaa
     4381 ggcactgagg atgagttact ggaaatagct atgggtattt tctatatgca agatagaaaa
     4441 cgtatccgcc tgtttgccag agcagatgtt tacaagcgat ttatttcatg cctggtatat
     4501 gttcccaagg atcggtttaa tactgaatta agatatgcca tgcaaaaaat tttggcagac
     4561 agttttaacg ctgaagagat taccttttct actcagtttt cggaatcggt attggcaaga
     4621 atacatttta tagtaagggt taatcccaag aatttacccg attttgattt aaaagaaata
     4681 gagcaaaaat tgattgaagt ggggcggtcg tggattgatg atttgcaaca tcatttacat
     4741 gaagtctatg gcgaagaaca agccaatgca ctttactctc ggtataaaaa tgcgtttcca
     4801 atatcttatt ctgatacctt ttcacctaga acggctgtct atgatattaa gcatattgaa
     4861 atgctaagcc ctgaaaatcc gctcggtatt aatttctata agccattgga tgaatctgag
     4921 aagagcttta ggttaaaggt ttatcagcat gacaccacta ttcctttgtc tgatgtatta
     4981 cctatcctgg aaaaacttgg tttaagagca attagtgaac gtccttatgt tttaaaattt
     5041 gaagatggta aagtagcatg gattaatgat tttgccatgc aatacaacaa agaaagcgag
     5101 tttaatatcg acgaaataaa agagttattt caaaatgcat ttgccagagt atggtttggt
     5161 gatgcagaaa atgatggctt taatttattg gtattagctg ccggattaaa ttggcgggaa
     5221 gttgctgtat taagaaccta tgcaaaatat ttcaggcaaa taggatttac atttagccag
     5281 gattatatgg aaacagcact aaataataat gtaagcattg ccaagaaatt agttaggtta
     5341 tttgaaattc ggtgcaatcc ccatgactcc aaaaggagag aggatagata cgttgcactt
     5401 gttgctgaaa tcttatctga tcttgataat gtagccaatc tggatgaaga cagaattata
     5461 agacagtacg tgcatgccat aggcgccact cttcgtacca atttttatca agtagacgct
     5521 caaggtaatc ctaagaatta tatttctata aaattgtcta gcaaactcat tcctggtgta
     5581 ccaaaaccat accccatgtt tgaaattttt gtttactcgc cacgatttga aggggtgcat
     5641 ttgcgctgtg gtaaagtggc tcgcggaggt ttacgttggt cggacagacg tgaagatttc
     5701 cgaaccgaaa ttcttggctt aatgaaagca caacaggtaa agaactctgt aattgtcccc
     5761 agtggcgcga aaggtggttt tgtacccaaa cagataccgg ctaatgctac ccgtgaagaa
     5821 attatggagg aggggatcag ctgttataaa ctctttataa gaggtttgct ggatataaca
     5881 gacaattaca aagaaggact cattgtaaaa ccccaaaatg ttgtttttta tgatgaagat
     5941 gacccttatc ttgtggtcgc tgcagacaaa ggcaccgcga cattttctga tattgccaat
     6001 tcaatttccc aggaatatga tttttggctt ggagatgctt ttgcctcagg tggctctgtt
     6061 ggttatgatc ataaaaaaat ggggattact gctaaagggg catgggaatc tgttaaacgc
     6121 catttctatg aattgaacag agatatccaa aacaatgatt tcactgtggt tggcatagga
     6181 gatatggccg gggatgtctt tggaaatggc atgttgttgt ccaggcatat caaactcgta
     6241 ggcgctttta atcatattca tatatttgta gaccctaatc ctgaagctga agctagcttt
     6301 aaagagcgtg agcgattatt caatcttccc agatctaact ggacagacta tgataaaaaa
     6361 ctcatttcca aaggaggagg gattttttct cgtagtgcaa aatctatccc ggttagccct
     6421 gaaatgcaga aagtatttgg aataaaacaa acttcgattg aacctaatga tctaattaaa
     6481 gcaatattaa aagccgatgt ggatttactt tggagtgctg gaattggaac ctatgtcaaa
     6541 tcgagtactg aaagtaatac cagcgtagga gatcgaacaa acgatgcaac tcgtgttaat
     6601 gccaatcagt tgagatgcaa agttataggc gaaggtggga atttgggatt aacccagtta
     6661 gcacgagtgg agtatagttt acacggcggc aaagtctata cagatttcat agataattct
     6721 ggtggagtta attgctcaga caaagaagtt aatattaaga ttttattgaa taatgttgta
     6781 tcagctgggg atctgactcc aaaacaaaga aatgagcttt taagtaatat gactgatgaa
     6841 gtagctaaat tagtactcag agataatttc ctacaaaccc gagccattag cttaactgct
     6901 tctcaagcct taagagccat tgagttacat gcaagatata ttaacgaact ggaaaaaaca
     6961 ggaaaaattg acagaacttt agagttttta cctgatgaaa aagtcctaat ggaacacaaa
     7021 ctaatgggga aaggattaac tcaacctgga attgctgttt taatgtgtta cagcaaaacc
     7081 atattaaaag agcaagttct tgcttctgaa gttcctgaag aagattatat gaatcaaata
     7141 cttattggtt catttcccaa acctctgcaa gagcgtttta gtaaacaaat gcaagatcat
     7201 cccttaagaa gggagataat tgctacccgt ttaagtaaca ttattgttaa tgaaatgggc
     7261 ttcacgtatg tttacaggtt acaagatgaa actggagcct ctgttgcggc aattgtcagg
     7321 gcctatatga ttgccagaag tgtcttgaat ctggaagcaa tatggaaaca aattgaggaa
     7381 cttggtacaa aaatcagcgc gcaagctcaa gttgatatga tgatgctgta tgttcgactt
     7441 tccaggagag tgacaaggtg gtttttacgg acacatagaa ggtcgatgag tataacgcag
     7501 gcgatagagt tatatgcaaa aggggttgat gaactgaaaa aatccatgcc tgccgttttt
     7561 ggtgaaacag gccggattca atatgaggag cattaccaag agtgggtaaa agaagggata
     7621 ccacctcaac ttgctcatga attaacagtg actcgaggct tatttgctgc aacagacatt
     7681 atagaaattg cctacgagga aaatataaaa atatcaaaag tagctgaaat atattttggt
     7741 attggagaat ttcttgatat agcttggctt agaacccaaa ttattgttca cacaacagaa
     7801 aatcattggg aatctttatc tcgagaggca ttacgcgatg atttggattg gcaacaacgc
     7861 cagttaacag ctgccataat gggttttgag cctaataata aggatttgca agaacgatta
     7921 acaagatggg gagaaacaca cgtttcttta attgataggt ggaattatat tctgacagct
     7981 ctgaaatcaa gtacagcttt gaactatact atgtttttag ttgccacccg agagttgctg
     8041 gatttaaccc aaacaacatt acaatccaat ttaaaaatgg aagagtcatt ttgacatgaa
     8101 aggagttaaa tgaaatggag gctaaaactg cttcagaata ttctacactg agcaaattat
     8161 ttcattggat cattgcattg gcagttataa tcatgctaat agcaggtttt tttcttgatg
     8221 aaatacctga gcaatttcag ggggttgctt atatgcttca taaatcaaca ggaattacta
     8281 tccttttctt gatgattttg aggtttattt ggatccatgc taaaggaaag cctgctttac
     8341 cagcgtctgt taaatcttgg gagaaaatat tatcaagatt tgtgcaatat ggtttttatg
     8401 cgctcttgat tattatgcca ttgagtggtt ggatcatgtc ggttgcagca gacagaatac
     8461 caaattactt tggcttgttt aaagcgccac ttccatggat tgaacctaat aaatctctag
     8521 caaaattaat ggctgaatcc catgaaatta ttgcatggat tttaatcgcc ttcatcacac
     8581 tgcatgtttt gggtgctctt aagcatcatt ttattgataa agacaatgta ttgaagcgaa
     8641 tgctgcctgg aaaaagcaaa tagccctctg tttttgaaaa ttgagcctga acttcaaagt
     8701 tcaagctcaa tttttccttc taattggatt ggaaatttat tgccttccaa acagacagat
     8761 gaaaagttct cttttaatat acttgcagca accaaatatc gattttcacc aagcatggag
     8821 taatcaatta actctccaac ctctgtgttt tcatccgctt taaataattt ttgaccagaa
     8881 tataattgat tatctgactg aatcacaaat ttctttaatt catgcttaag agtggctcta
     8941 tagtgagttc ttgcgatgat ttcctgacct ttataacacc ctttatcaaa actgacgtac
     9001 gcagtttgat gtaagccaat tctatgtggt aaaaagagcc ccctagaatt agggtaaata
     9061 tcaatttgat tgttaaataa tctaagagta tgccaggtta atgaaccaag taattgatta
     9121 ttttcaataa aaggcttgca caaactatca taatagtctg aatggattaa atagatatag
     9181 aatcctttgc ctaagtgata aacacaacca tgagtcgtac aagataaagc atacaattcg
     9241 ctgggcaaag gtagtagctg tggtatttga tcattatcat gctgtaaata aaagcctaaa
     9301 attttatagt tgttatttga tgtgatttta acacgagaaa gcagtgcaac tttgtttaat
     9361 gaattctgag ttacttcaat taaatcttgg ggcagaacta attttacacc ttgccagttg
     9421 ataatatcca ataaggaaag aattctccct ttaaggttac attgggcccc ttgaaccatt
     9481 gaaacatccg ataccaatcg taaatcacaa gttaactgcc cttgcagaaa atctattgat
     9541 ttctctccaa acaaatcgat gacacctaga taggataagt caaaaagata gtttttctgc
     9601 ttatttaata ataattcaga ctctataggg cggaatgtta ttaaagacct attattcact
     9661 aaatgttcta gcatagtttt catatcaaga tcagtgatgt tgtttataat acagagtagt
     9721 gtatcatttg gcctctcaaa atcagatatc attatcgaat aattagggtt atgttgtatt
     9781 tatcatgaag taatagagga aatccattgt ggacaataga gaaaaatcaa ggttattatg
     9841 gaaatgcagg agaggaatgc tagagcttga tcttgtttta cagaaattca tagccaatga
     9901 gattgatcga cttacagaaa atcaattgaa agcatttgat aatctattaa cccacaatga
     9961 ccctaattta tatgcctggt taatggggca tgaggaaccc gaaaaagagt tgcttgaaat
    10021 tgtatcattc atcagaaact gtgattaaac caggtaaatc caaagtatat cttcgattaa
    10081 ttgcgattct ttatttgtgc accattggat tagttttata ttcttcttta cctgtgttga
    10141 tagaaggcgt cttaattttt cttatcgtta ttcaatttaa aaatgatgta acttaccaaa
    10201 gtgcttgcca aaaagttaaa gaaatcaaat acttgaaaga ccttacgtgg tcactggaaa
    10261 caatagatgg tactacccat cattatgaca aactaaaaat tcttattcat aatatgctat
    10321 ttcaagttat tcaattatct tcagataaaa aaaat
//
LOCUS       DBNCWK010000109        10343 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.109, whole genome shotgun sequence.
ACCESSION   DBNCWK010000109 DBNCWK010000000
VERSION     DBNCWK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.109"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            249..2789
                     /gene="mutS"
                     /locus_tag="AC06IO_002261"
     CDS             249..2789
                     /gene="mutS"
                     /locus_tag="AC06IO_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIO9875436.1"
                     /translation="MASSHTPMMQQYLRIKTDYPDMLLFYRMGDFYELFFDDAKRASQ
                     LLDLTLTHRGQSADKPIPMAGVPYHAVENYLARLLKKGESVAICEQIGDPATSKGPVE
                     RQVTRIITPGTVTDEALLDARKDNILLAIHTQKQKIGIAWVDLGGGRFHLQELTEEHQ
                     LNAELVRLQPAELLCKESTPLPSFCSNFAVKFRPGWEFDASNAHKLLCEQFSVTDLSA
                     FGEQNYPTALIAAGALLAYLKTTQKQSLPHLTTLTLEQSEDYLQLDASTQKHLELFEN
                     IHGGGEHCLLSILDKTACAMGSRLLKRWLGKPLKQHAIIQTRQQAIKEIIFLQQDVSL
                     HQLIKQCADVERIVSRIALKSARPRDLVSLLQTLTLLPAIHDELQENKSLLINEIKKE
                     ISPLPLLQQLLETAIIDNPPMLIRDGGVIAPGFDEELDELRNLSSNAHETLVKLEQEE
                     KNRTGLSTLKLGYNSVQGFYIELSKAQAQNAPPHFHRKQTLKNVERYITPELKLFEDK
                     VLSAQSKALAREKWLYDNLLEEIQQYIPELSDLAKSLAQLDVLVTLAERAQSLNWNCP
                     NLVPESGIMIQAGRHPVIEPLLQERFIANDLELKPNQNMLLITGPNMGGKSTYMRQTA
                     LIVLLAHIGSFVPADKVTLGPLDRIFTRIGASDDLSSGRSTFMVEMTETAQILRQATS
                     QSLVLIDEIGRGTSTYDGMALAYASCAFLASTIKAYTLFSTHYLELTELPKEFSCIRN
                     VHLQASIKTGQIVFLYRVEEGCANRSYGLEVAELAGIPKEVLKLAHEHLNQIQDTQSI
                     LVQTQIIKPPTSPVLTELKKIDPDRLTAKEALDLIYKLKHLECAESIN"
     gene            2802..4490
                     /locus_tag="AC06IO_002262"
     CDS             2802..4490
                     /locus_tag="AC06IO_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0097347 - TAM protein secretion complex
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HIO9875437.1"
                     /translation="MNRFAYLIILIILYFPLFAQDKKAGLLEIDGIKGKVLANVETRL
                     GELSQIKPLSQFTPTELQDQINKAIQPFGYFNAETSINNLNNKIIIKVQPGSQIRIAS
                     IKAMLTGEGAQNPLLRKTLKELPLHIGDPLFSEQYEKAKQNIINTAENMGYLHGVFKK
                     AEILIDEHKKSAQITLIFDTGPQYYFGQVQFDPTYISPQLLHRFVPFDPGQPYATDQV
                     LKLNDYLSNSGYFSSVLVKPQITDAQTVPVIVHLQPVPKYSYSFGLGYGTDTGVRGKA
                     ALHVIPVNRQGHKFNAVAQGSFRQNALQAQYVIPGKNPVTDQYALTGNFSNLNYNAGY
                     SNATLLSLSQLHNVNQFQRALSLNALYESFHYSLQPNTDQFLLYPKANITFSKTKNLL
                     FSPSGYNITFNALGANKAVLSHLNFGQLSLDAKAALTLDSLHLRLYGHTIQGITAIND
                     INELPLSLALLLGGTDNLKAYSFNSIGPGKIITYGGFEIQKEFKKNWYLVGFYDAGDV
                     YNPSVKNIQYDIGGGLMWVSPIGPIKVGLAQSVDNKMERIGHNPRLVISMGPDL"
     gene            4487..7027
                     /locus_tag="AC06IO_002263"
     CDS             4487..7027
                     /locus_tag="AC06IO_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIO9875438.1"
                     /translation="MMILKFIKKGFYYCLITAICLVSLVIFLISTTPGLYAVIKLTHL
                     YLPGTLKIHHLKGQLLDQFSIGEMEYQHQGTKIKIFNLAVNWRFSSLLHKKLPIDSMS
                     AEMIEINQKTPMLILKNIKLSGYLNQQSIKLNTLQFNYWNQLITSQLELSNSFPHVFS
                     GKIRLNPHSTNKNIFSGGIDIGGNRNQIQWGGEFEGPGKITINGSLQQLKQIKQVIKW
                     RDLHWQDNKNNNLKSSQGRIEVSGTIPNLAIDLKSKLDIDQLKNWQMNASIKGKLPWQ
                     WNVDATFSQPLDFSSKLDGLYTKFALKMETQNDHHADFLITIAPGHYQITENNLMPLI
                     QFTGGNIKGSLSQKQFSGQGVLFLDAYKKVNFNFKFPEFNVYQDIAQQTIDAKLSILF
                     NSLDFLQNASPYLINPKGQFLASIHAKGTLNKPIIESKLALNKGSFNIPALGLNITDM
                     VLAVFSKKDGWEATGSIHSSEKKLTLKGQGIFNKELGGTVSIEGSNFPIANNKEFQVN
                     ISPQLQVRFTPSLCQITGIIQIPNAHIKPQTFTNSLVATDDIVFASKNAQPITPLFNT
                     NMSVRVEMGEEVELTFKGLHAYLAGIVTINQLSQGPITANGELTVKKGEYKAYGQDLS
                     IEQGQLIFTGGRIDNPGINLKASKNISNSFGSVSGSSELFNFNNYNNLNVGNNFNVGV
                     EVTGRLQSPKIQLFSNPPTLSQADILSFLVIGRPANQADKAKGQLLLTAISSMNLGTG
                     TNGTQLLEQLKQKLGFDFNVQTTTNFNQQTNKISESTGVVVGKSLSDRIYLSYNVGLS
                     QTDPNVLTLKYILNKFLSIQVSSSDSGNGVDILYTKSTSSPEKAKPDK"
     gene            7061..8056
                     /gene="hemB"
                     /locus_tag="AC06IO_002264"
     CDS             7061..8056
                     /gene="hemB"
                     /locus_tag="AC06IO_002264"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIO9875439.1"
                     /translation="MNEYNLPLRLSRLRKNAMSRALVRETRLHTQQLIAPLFISEYLT
                     EAKEISAMPGQFQLGLNCIPEEIEALSKLGIPAVLLFGIPKQKDACGSESMNNNGIIQ
                     QAIKKIKSVNSDMLVITDLCFCEYTDHGHCGILDGTQINIAATLELLGKQAVSHAEAG
                     ADWVAPSGMTDGMVLAIRQALDTASFHDIPILSYSAKYCSCLYGPFREAAQGAPQFGD
                     RKAYQMDPANSEEAVRETRLDLEEGADMIMVKPAGFYLDIIYKLKQHFADVPLCAYQV
                     SGEYSMIKNAAKNNLINEEQAMTESLIAIKRAGADFIISYFAKGMAELLNSQNFI"
     gene            8193..8585
                     /locus_tag="AC06IO_002265"
     CDS             8193..8585
                     /locus_tag="AC06IO_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875440.1"
                     /translation="MNLMLKGLSALAISLGAATTFAAPAYLTTHNRTGEESNAYIAGS
                     IPSLYPTAAYSTNQVYWNLVRLACYGHTTNGQCPALIKMATNTANPIDIGYVTMDLNT
                     GDITPKTLSAKGYSLRVIGPGEAEITKN"
     gene            complement(8883..10325)
                     /locus_tag="AC06IO_002266"
     CDS             complement(8883..10325)
                     /locus_tag="AC06IO_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIO9875441.1"
                     /translation="MKIRHKPLRTLVSTAVLGVLATGTLNAGGFSLYTEGAGYTTGNF
                     AAGVAAEAADASTGWYNPAGLVLIRDQQAVFAGVGVFPTAKLSGVSTYSTINPPPFPS
                     FVYSEVFNNINGAEDAFVPSFHYALPLGENATFGLSVVSPFGLATDWGPDSPVRYQAT
                     FTELITTNISPEIGGRLTEHVALGAGLDLQYARVKYNRILGVPNILNVLPPFNPMLAD
                     SLTYNKGHSFGVGFHVGVLGMFNDNHTRLGANYQSSMRHEFYGYSRLTGPLASPGNIL
                     APFLFPAPGIFQSDDLFSNSIDFPDVVTISAYHDVNDKLALLGSLVYTAWSSYKTVQL
                     SNAAAGRPADNGNVSQAKAISISPQNYNDTLRVAIGANYHVNDKLMLRVGGGYDPTPT
                     NNIDRDVRLPDTDRWALSAGAHYQATNNIGLDIGYTHLFTSSDPVINRTDAFTATTRY
                     NVTASGTAGANLVGVQATWTIDKEPPAPTK"
BASE COUNT         3435 a         2060 c         1882 g         2966 t
ORIGIN      
        1 atattttatt gcagtatgtt aaattaaatg acataaacca tgccttagta atcattttgg
       61 caaattctat agtaatttgc agattttaaa aatcatactt attttttaga cacatcaagg
      121 ttatattaaa tgctttttga tcacattgtc tttattgttt tcataaaaat caataccacc
      181 aacatgcctt actgacttcg cacaacactt ttgttaaaat tggggcaatt tcaatcggct
      241 caatcactat ggctagctct cacacaccaa tgatgcagca atatttgcgc attaaaacag
      301 actatcccga catgctttta ttttatcgca tgggtgattt ttatgagttg ttttttgatg
      361 atgccaaacg cgcgtcacaa cttttggatt taactctgac acatagagga caatctgctg
      421 acaaacctat tcccatggcc ggcgttcctt atcatgcggt agaaaactat ttggcacgat
      481 tattaaaaaa aggggaatcc gtagccatct gtgaacaaat cggagatcca gctaccagca
      541 aaggaccagt agaaagacaa gtaacgcgaa tcattactcc tggtacagta acagatgaag
      601 cattattaga tgccagaaaa gataatattt tgttggccat acacacccaa aaacaaaaaa
      661 ttggtattgc atgggttgat ttaggtggcg gtcgttttca tttgcaggaa ttaacagaag
      721 aacatcagtt aaatgcagag ttagtgcgcc tgcaaccagc agaactcctc tgcaaagaat
      781 ccacgccgct tccttcattt tgttctaact ttgctgttaa attcagacca ggatgggagt
      841 ttgatgcaag caacgcacat aaattattgt gtgaacaatt ctccgtgacc gatctttcgg
      901 cctttggcga acaaaattac cccactgcct tgattgcagc aggcgcttta ttagcttatt
      961 taaaaaccac tcaaaaacag tcattaccac atcttacaac acttactctt gagcaatccg
     1021 aagattacct gcaactggac gcatcgactc aaaagcactt ggagcttttt gaaaatatac
     1081 atggtggtgg tgagcactgt ttgctttcca tattagataa aacagcttgt gcgatgggaa
     1141 gccgtttact caaacgttgg ctgggaaaac cattaaaaca acatgcaatc attcaaacaa
     1201 gacaacaagc cattaaggaa attatttttc ttcaacaaga cgtttcactt catcagctga
     1261 ttaaacaatg cgccgatgtg gagcgcatag tatcacgtat tgctttaaaa tcagcgcgcc
     1321 cacgtgattt ggtttcctta ttgcaaactc tgacactgct tccagcaatc catgacgaat
     1381 tacaagaaaa caagagcttg ttaatcaatg aaatcaagaa agaaataagc cctctcccac
     1441 tgctgcagca actgcttgaa acagccatta tagacaaccc gccaatgcta attagggatg
     1501 gtggagtcat tgctccagga tttgatgaag aactggatga gttgcgtaat ttaagtagca
     1561 atgctcatga aacattagta aaacttgaac aggaagaaaa aaatcgcaca ggattatcca
     1621 ctctaaaact aggttacaat agcgttcagg gattttatat tgaactgtca aaggcacaag
     1681 ctcaaaatgc gccgccccat tttcacagaa agcaaacttt aaaaaatgtg gaacgttata
     1741 ttactcccga actaaagctg ttcgaagaca aggtgctttc agctcaatcc aaagcattgg
     1801 ctcgcgaaaa atggctatat gacaatttgc ttgaagaaat tcagcaatac atacctgagt
     1861 tatcggatct tgcaaaatct ctggcgcaat tggacgtatt ggtcactctt gctgaaagag
     1921 cacaaagttt aaattggaat tgcccgaatc tcgttcctga atcaggcatt atgatacaag
     1981 ccggacgaca tcctgtcatt gagccattat tacaagaacg gtttatcgct aatgatttgg
     2041 aacttaaacc aaatcaaaat atgttactaa tcactgggcc caacatgggc ggcaaatcga
     2101 cctacatgag gcaaaccgcc ttaatcgttc tgttggcaca tattggaagt tttgtacctg
     2161 ctgataaagt gacattaggt ccattagacc gaatctttac tcgcattggg gccagtgatg
     2221 atttatcctc tggacgttcc acttttatgg tggaaatgac tgaaacagct caaatattaa
     2281 ggcaggcaac cagccaaagc ctcgtattaa tcgatgaaat tggtcgtggt accagtacct
     2341 atgatggaat ggcattagct tatgcaagct gtgcctttct tgcgtcaaca ataaaagcct
     2401 atactttgtt ttctactcat tacctggagt tgactgaatt acccaaagaa ttctcttgta
     2461 ttcgcaacgt gcatttgcaa gcatccatca agacaggaca aattgttttc ttgtaccgtg
     2521 tcgaagaagg ttgcgcaaat cgtagttacg gattagaagt cgcagaatta gctggcatac
     2581 ctaaagaagt gctaaagctg gctcatgaac atctgaatca gatacaagat actcaatcta
     2641 tcctggtcca gactcaaatt ataaagcctc ccacatctcc agttttaact gaactaaaaa
     2701 aaattgatcc agatagatta actgccaaag aagcgttgga tttaatttat aagttaaaac
     2761 acctggagtg tgccgagagc atcaattgat gtagttatta catgaatagg tttgcatatt
     2821 taatcatact aatcattttg tattttccat tgtttgctca ggacaaaaag gccgggctgc
     2881 ttgagattga tggtataaaa ggaaaagttc ttgctaatgt ggaaactcgt ttaggtgaat
     2941 tgagccaaat taaaccttta agccaattta ccccaactga acttcaagac caaattaata
     3001 aagccattca accatttgga tatttcaatg cggaaaccag tattaataac ctcaacaata
     3061 aaataataat aaaagttcaa cctgggtctc aaattcgtat tgcctccatt aaagcgatgc
     3121 taacaggaga gggagcacaa aatccattat tacgaaaaac tctgaaagag ttacctctgc
     3181 atattggaga tcctctgttt tcagaacaat acgaaaaagc aaaacaaaat attataaata
     3241 ccgcagaaaa tatgggatat ttacacggcg tatttaaaaa agctgaaata ttgatagatg
     3301 aacacaaaaa atcggctcaa atcaccctga tttttgatac ggggccccaa tactattttg
     3361 gccaggtgca atttgaccca acctatattt ctcctcagct attacatcgt ttcgtaccat
     3421 ttgatcctgg ccaaccttat gccacagatc aagttttgaa attgaatgat tatttatcga
     3481 acagcggtta ctttagttct gtactggtaa aaccccaaat tactgacgcc caaacagtac
     3541 cggtcattgt tcatttgcaa cctgtaccta aatattccta ttcatttggc cttggctatg
     3601 gaacagacac aggagtgcgt ggaaaagcag ctcttcatgt gattccagtc aatcgccaag
     3661 gacataagtt taatgccgta gcgcaaggct ctttcaggca aaacgcctta caagcccaat
     3721 atgtaatccc cggaaaaaac cctgttaccg atcaatatgc cttgaccggt aatttttcaa
     3781 atttaaacta taatgcaggt tatagtaatg ctactctctt atccctgagc caattgcata
     3841 atgtgaatca atttcaaaga gcgctttcac tgaatgctct gtatgaaagt tttcattact
     3901 ctctacaacc caatacggat cagtttttgc tctaccccaa agccaatatc actttcagta
     3961 aaactaaaaa tctcttgttt tcgccatcag ggtataatat tacttttaac gcgcttgggg
     4021 cgaataaggc tgtcctctcc catctgaact ttggacaact ttcactggat gccaaagccg
     4081 cgctcacatt ggattcactg catttgcgcc tgtatgggca taccatccaa ggtattacag
     4141 caatcaatga tatcaatgaa ttaccgcttt ccctggcatt acttctggga ggaactgaca
     4201 accttaaagc ctatagtttt aactctatcg gaccaggaaa aattatcact tatggtggct
     4261 ttgaaattca aaaagaattc aaaaaaaact ggtatcttgt tggattttac gatgctggag
     4321 atgtttacaa tccatccgtt aaaaacattc aatacgatat tggaggcggt ctgatgtggg
     4381 tttcccctat tgggccaatt aaagttggat tagcccaatc agtcgataat aagatggaga
     4441 gaataggaca caatccaaga ttggttatca gtatgggacc agacttatga tgatattaaa
     4501 atttatcaaa aaaggatttt actactgctt aataacagca atatgtctgg ttagtttggt
     4561 tatctttttg atttcaacta ccccggggct atatgcagta attaaattaa ctcatttata
     4621 tcttcctggc actcttaaaa tacaccatct taaaggacaa ttacttgatc aattttctat
     4681 tggtgaaatg gaatatcaac atcaaggcac taaaataaaa atttttaacc tggcggttaa
     4741 ctggcgtttc agctcattgc ttcacaaaaa attgcctatt gatagcatga gtgctgaaat
     4801 gattgaaata aatcaaaaga cgcccatgct gatactaaaa aatatcaaat taagcggtta
     4861 tttaaatcaa caatcaatta aactgaatac actgcaattt aattattgga atcaattgat
     4921 taccagccaa ttagaactta gcaactcatt tcctcatgta ttttcaggaa aaattcgttt
     4981 aaacccacat tctacaaata agaatatatt ctcaggaggg atagacattg gtggtaatcg
     5041 gaatcaaatt caatggggag gagaatttga aggtcctgga aaaataacaa ttaacggcag
     5101 tcttcaacag ttaaaacaaa taaagcaagt cataaaatgg cgcgacttac attggcaaga
     5161 caataaaaat aacaatttaa aaagctcgca aggaagaatt gaagtatcgg gaacaattcc
     5221 aaatttagct atcgacttaa aatccaaact tgacatagat caattaaaaa attggcaaat
     5281 gaacgcttcg attaagggca agctgccttg gcaatggaat gtcgatgcta cgttctctca
     5341 accattagat ttctcatcaa aacttgatgg tttatataca aaatttgctt tgaaaatgga
     5401 aactcaaaat gaccatcatg ccgactttct gatcaccatt gctccaggtc attatcaaat
     5461 aacagaaaac aatctgatgc cgttaattca gtttactggc ggcaatatta aaggctctct
     5521 ttcccaaaaa caattcagtg gacaaggagt tctctttctg gatgcatata aaaaagtgaa
     5581 ttttaatttt aaattcccgg agtttaatgt ctaccaagat atagcacaac aaacaattga
     5641 tgcgaaacta tccattcttt ttaactctct ggattttttg caaaatgcca gtccttattt
     5701 aataaatccc aagggacaat ttctcgcctc cattcatgca aagggcactt taaataaacc
     5761 aataattgaa agtaaactcg ctttaaacaa aggctcattt aatatccccg ctttaggtct
     5821 caatataact gatatggtat tagcggtttt tagcaaaaaa gatggatggg aagcgactgg
     5881 ctctattcat tcatctgaaa aaaaactgac cttaaaagga caagggattt tcaataaaga
     5941 gcttggtggg actgtttcaa tagaaggttc caattttccc attgcaaata ataaggaatt
     6001 ccaggttaat atatctccac agttacaggt gcgcttcact ccatcgctat gtcaaatcac
     6061 tggaattatc caaattccta atgctcatat aaaaccccaa acatttacca atagtcttgt
     6121 ggctacagat gatattgtat ttgcgagtaa gaatgcccaa cctataaccc ccttatttaa
     6181 taccaatatg tcagtacgag ttgaaatggg agaagaagtt gagctgacct ttaaaggatt
     6241 gcatgcttat ttggcgggta tagttacaat caatcaatta tcacaaggac ccataacggc
     6301 aaatggtgag ttaaccgtca aaaaaggaga atataaagcc tatgggcaag atttatctat
     6361 tgaacaagga caattaattt ttacaggcgg ccgaattgat aatcctggaa ttaatttaaa
     6421 ggcatccaag aacatatcca attcatttgg gagtgtatca ggctcatccg aactgtttaa
     6481 ttttaacaat tataataacc tcaacgttgg taacaatttt aatgttggag tcgaagtaac
     6541 tggacgatta caatcaccta aaattcaatt attctctaat cctccaacac tttctcaagc
     6601 agatatccta tcttttcttg taataggccg cccagccaat caagcagata aggcaaaagg
     6661 ccaattactt ttaacagcta tctcctcgat gaatttaggt acaggaacta atggaaccca
     6721 actgttggag caattaaagc aaaaattagg attcgatttt aacgtacaga caaccactaa
     6781 ttttaatcaa caaaccaata aaattagtga atcaactggt gttgtggttg gaaaatctct
     6841 ttcagatcga atttatttaa gttataacgt cggtttgtct caaactgacc ctaacgtatt
     6901 aacattaaaa tatatactaa ataaattttt aagcattcag gtaagcagca gtgattcggg
     6961 taatggcgtc gatattctat atactaaaag cacatcatca ccagaaaaag caaaacctga
     7021 caaataaaat cttaatccat actgtcgaag gatgtaataa atgaatgaat ataatttgcc
     7081 tttaagatta tctcgtttaa gaaaaaatgc catgtcaaga gcattagtaa gagagacgag
     7141 gctgcataca caacaactaa ttgctccatt gtttattagc gaatatttaa ctgaagcaaa
     7201 agaaatcagc gctatgccag gccaatttca actaggcctc aattgtatac ctgaggaaat
     7261 tgaagcttta tcaaaattag gcatccctgc agtcctctta ttcggcatcc ctaagcaaaa
     7321 agatgcttgt ggcagtgaat caatgaataa taatggaatc atccaacaag ctattaaaaa
     7381 aattaaatca gtgaatagtg acatgttagt gattactgat ctttgttttt gcgaatatac
     7441 cgatcatggt cattgcggga ttctggatgg tactcaaatt aatattgctg ctaccttaga
     7501 attattagga aaacaagcgg taagtcatgc tgaagcaggt gctgactggg ttgctcccag
     7561 tggcatgaca gacggaatgg ttttggctat acgccaggca ttggatactg cctcatttca
     7621 tgatattcct attttaagtt acagtgccaa atattgctcg tgcctttatg ggccttttag
     7681 agaggcagcg cagggtgcac cacagtttgg cgataggaaa gcgtatcaaa tggatccggc
     7741 aaatagtgag gaagctgtaa gggaaacccg attagatctg gaagaaggcg cagatatgat
     7801 tatggttaaa ccagccggtt tttatcttga tattatttat aaacttaaac aacattttgc
     7861 tgatgtaccg ctttgtgcct atcaggtcag cggtgaatat tcgatgataa aaaatgcagc
     7921 aaaaaataat ctaatcaatg aagaacaagc aatgactgaa agtctcattg cgattaaacg
     7981 tgctggcgct gattttatta tttcttattt tgctaaaggc atggctgagt tattaaactc
     8041 tcaaaatttt atttaattat gagtcaaata aatcaaatca agaactataa aacgactata
     8101 taaagagaaa gacttgcctc atctataaca aatctcatat agtaaatggg attggcattg
     8161 cctaatcaac tacaatctag aaggagaaat caatgaattt aatgcttaaa ggtttatcgg
     8221 ccttagcaat tagtctcggt gctgcaacaa cttttgcagc tccagcttat ttaacaaccc
     8281 ataacagaac tggtgaagaa tccaatgcct acatagcagg cagcatcccc tctctttacc
     8341 caacagcagc ttactctacc aatcaagtgt attggaattt agtgagattg gcttgctatg
     8401 gacatacaac taatggtcaa tgccctgcct taattaaaat ggcaaccaat acagctaacc
     8461 caattgatat cggatacgtt accatggatc tgaatactgg cgatattact cccaagacgc
     8521 tatcggctaa aggctattcg ctgagagtaa taggacctgg agaggctgaa attactaaaa
     8581 actagtcaat tgtctccatt tgttttgaat agcttatgta ggttgaacgc taatacaacc
     8641 tacataatca atacatgtaa atacttacgt ttatcttaat taagattaat tatctcagca
     8701 cacgattatc taaacgttat ttatcccgac acacataaaa ctaacagggc ttatacaaaa
     8761 ccccaaggtc agtgcaaaaa atatttccta attttagcga agagattggg atttttcata
     8821 agttctgact aataaaaaag ccacgcatat cacgtggctt ctaacaaggt caagagaaag
     8881 ctttatttag tcggtgctgg aggttcttta tcaatagtcc aagttgcttg cacacctact
     8941 aaattggcac ctgcagttcc agatgctgta acgttatatc tggttgttgc agtaaacgca
     9001 tcagtcctgt taataactgg atcacttgaa gtaaataaat gggtatatcc gatatcgaga
     9061 ccgatattat tcgttgcctg ataatgagcg ccagcagata aagcccaacg atctgtatcc
     9121 ggtaaacgca cgtctctgtc aatattgttc gtaggtgttg gatcataacc accgcccaca
     9181 cgaagcatta atttatcatt aacatgataa ttggcaccaa tcgctacccg caaagtatcg
     9241 ttataattct gcggtgatat gcttatcgcc ttggcttggg aaacatttcc attatccgct
     9301 ggtcttccgg cagctgcatt agagagttgt actgttttat aactactcca tgcagtataa
     9361 actaaagagc ctaacagtgc aagtttgtca ttgacatcat gataagcact aatagtcact
     9421 acatcaggaa aatcaattga gttactaaat aaatcatcgc tttgaaatat gccaggtgcc
     9481 gggaacaaaa atggcgctaa tatattacct ggacttgcca atggccctgt tagacgactg
     9541 tagccataga attcatgccg cattgatgac tgatagtttg caccgagacg agtatgatta
     9601 tcgttaaaca tgcccagaac acccacatga aaacctactc caaaagagtg tcctttattg
     9661 taagtcagtg aatcggctaa catggggtta aaaggtggca aaacattcag tatgttaggt
     9721 actccaagta tacgattgta tttaactcta gcatattgca aatccaaacc agcccccaaa
     9781 gcaacgtgct ctgttaatct accaccaatt tcgggtgata tattggtggt aattaactca
     9841 gtaaaagtgg cttgatagcg cactggagaa tctgggcccc aatctgttgc caatccaaat
     9901 ggtgatacaa cacttaaccc aaacgtagca ttttcaccca atggcaaggc ataatgaaac
     9961 gagggcacaa aagcatcctc agccccattg atattattga aaacctcaga ataaacaaaa
    10021 gaagggaaag ggggcggatt aattgttgag taagtactaa ctccgcttaa cttggcagtt
    10081 gggaatacac cgacacctgc aaatacagct tgctgatcgc ggattaatac caaacctgct
    10141 ggattatacc aacctgtaga agcatccgct gcttcagcag ccacaccggc agcgaagtta
    10201 cctgttgtat atccagctcc ttcggtatac aaagaaaatc cacctgcgtt taatgtacca
    10261 gtagctagaa ctccgagaac agcagtactc actagcgttc ttaagggttt gtgcctaatt
    10321 ttcactccac actcctttcg ttt
//
LOCUS       DBNCWK010000110        10307 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.110, whole genome shotgun sequence.
ACCESSION   DBNCWK010000110 DBNCWK010000000
VERSION     DBNCWK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.110"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            91..342
                     /locus_tag="AC06IO_002267"
     CDS             91..342
                     /locus_tag="AC06IO_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875442.1"
                     /translation="MNKLQLTGAALAVGAASMFALAPAFAVDATTTTIHCKGVNACKG
                     QGSCKTADNACKGQNSCKGKGILEMSKEDCEKQGGTVAE"
     gene            418..1281
                     /locus_tag="AC06IO_002268"
     CDS             418..1281
                     /locus_tag="AC06IO_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HIO9875443.1"
                     /translation="MEVSPNKSAQKLPYLGFGLGLRPNYYEEILTSKPDLDWFEILTE
                     NYLIPGGKPLYYLDKIREHYPIVMHGVSLSLGSTDPLNWDYLNQVQELASRIEPVWIS
                     DHLCWTGVHGLNTHDLLPVPYTTEAIQHIVSRIQEIQDFLKRPFLIENVSSYLTYKQS
                     EMSEWDFILEIVKQSGCYLLLDVNNVYVSSFNHNFDPMAYINSMPPGRVAQIHLAGHT
                     NHGDYIIDTHNAPVIEPVWDLYEATIQRLGPVSTMIERDDNMPDFSELLSEINHAKRL
                     AIQAAKEKVAV"
     gene            1278..2066
                     /locus_tag="AC06IO_002269"
     CDS             1278..2066
                     /locus_tag="AC06IO_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HIO9875444.1"
                     /translation="MKSLLTLQTQFQEFIYSGQGSIEESIVPTKLVSTEMRLNIYKDG
                     YRLRLIECLAANYPALYAYLGTEEFAKLSARYIDSYPSAYRSIRWYGDRLPELIKKYY
                     AKSCYYLYELAELEWKMTLAFDAADDTVLNVEEMAAVPPESWAEMGFVLHSSVHRLDF
                     CWNVFSLWQALVNNLEIPELKDSPAATGWVLWRGQNYIIQYYSLSEEEAWALDALIQG
                     SSFGVICEGLCQWMPAEEVGMKAASYLKNWIQNGLLSQCRIRRN"
     gene            2118..3491
                     /locus_tag="AC06IO_002270"
     CDS             2118..3491
                     /locus_tag="AC06IO_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIO9875445.1"
                     /translation="MLNPREIRTIEDAKRIVEERQLTHVKVGLFDIDGVMLGKYMSRN
                     KFFSALEHGFAFCDVILGWDSKDKLYDNVKYTGWHTGYPDASVRIIPSTCREILFEEN
                     QLLFMAEFSQAAEAICPRGILRRVINKAAGMGYDVYAALEYEFFVFDETPDSVRAKGF
                     RNLKPVTPDNFGYSIIRNTVHAEFYHQILTMAEKMDFPIEGLHTETGPGVLEAAIAVD
                     DAEAAGDKAALFKTFMKILAQRNNKMATFMAKWSGDYPGQSGHIHLSLRDRDGGKSAF
                     YESGREYNMSKIQRHFLAGQQKLMPEFLAMLSPTVNSYSRLIPGYWAPTEATWGVENR
                     TTALRVIPGSDKSQRIEYRLGSADANPYLALAAAIGSGLYGIENELEPEAEVKGNSYE
                     QHHSPALALPRTLWESAQNLKNSSAAYQLFGQEFVEHFAASREWEEREFRKHITDWEL
                     DRYFEII"
     gene            3529..4926
                     /locus_tag="AC06IO_002271"
     CDS             3529..4926
                     /locus_tag="AC06IO_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIO9875446.1"
                     /translation="MIATLKTYSPVDNSLYVEREYANQEEIKTALSKAQVAKKLWSLS
                     SLAERQEYCLAAVNEIVASKVDIAEEICWQMGRPIRYAAGEINGFEERARYMIAQAES
                     SLSSVELPEKEGFTRYIKREPLGLVLVIAPWNYPYLTAVNAIIPALMAGNVVILKHSA
                     QTPLVAERFDQAFKKAGLPMGVFQYLHLTHEDTEQLMRSPAVNSVAFTGSVAGGERVE
                     QTVAGRFIPVNLELGGKDPAYIRADADLDHAVETVIDGAFFNSGQSCCGIERIYVHKE
                     IYEQFVNKAVNLVKQYKLGRPDDPETTLGPLVRASSADFVREQIKEAVAQGAVAHINT
                     RDFVMDRPGTPYLAPQILTDVNHRMRIMTEETFGPVVGIMSVDSDEEAIERMNDSDYG
                     LTAAVFTQDIHRGIAIGERLQTGTFFINRCDYLDPGLAWTGVKKSGRGCTLSAIGYEY
                     LTRPKSFHIRTKIET"
     gene            5056..6216
                     /locus_tag="AC06IO_002272"
     CDS             5056..6216
                     /locus_tag="AC06IO_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIO9875447.1"
                     /translation="MKNQSLIVNWNYPTRILVGAGRINELAKVCRDLGMNAPLLVTDP
                     GLASSLMVSKVIQQAQASGLRTGLFCQIKTNPTGDNVMNGVYAFKAGQHDGVIAFGGG
                     SALDAGKAIALMVGQDRPLWDFEDVGDNWTRVNVSAMAPVLAIPTTAGTGSEVGRASV
                     ITDAEKQVKKIIFHPKMLPAMVILDPELTVGLPKSLTAATGMDALSHCLEAYCSNYYH
                     PMAEGIALEGIRLIKENLIQAYKDGSDLEARTHMLVASAMGATAFQRGLGAMHALAHP
                     LGAIYDVHHGRLNAVLMPYVLLANRSEIENKIERLANYLSIANGFDGFLDWIEQMRLE
                     LGIENTLSQLGIDHSQIDRLAKMATEDAAAASNPVLFTLEQYKELLSKAIGE"
     gene            6346..7044
                     /locus_tag="AC06IO_002273"
     CDS             6346..7044
                     /locus_tag="AC06IO_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIO9875448.1"
                     /translation="MNLGILLCDKVSEVFVADHGQYPEMFANLLRPADSTLEFTVFDA
                     EHGELPTDIHAVDAYLISGSRHGVNDDYPWIRKLEEFVRTLHASQKKLIGICFGHQLI
                     AKALGGKVIKSPKGWGVGMSQNQIYQFKEWMRPSLNCFNLLVSHQDQVIELPTGAEIL
                     AGSDFCPNYMMQVGSFFSVQGHPEFTKSYSRDLMVSREDSVNEIEFAKGMKSLELHEN
                     DTIIAQWIVNFLKA"
     gene            7135..9369
                     /gene="lem21"
                     /locus_tag="AC06IO_002274"
     CDS             7135..9369
                     /gene="lem21"
                     /locus_tag="AC06IO_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem21"
                     /protein_id="HIO9875449.1"
                     /translation="MAKTIKATGDGACLFNAVSIGLSVEILSGRLDSQLDTPGYQALL
                     DEFAKHHPQFNPKSWKTLKEWLAYYNDTRDIELILAPVLFNLNQKYQDHLDEEILNEL
                     TNLVWKNKANIENGQAWFQLQNTGDLGEALFPKLENLDLKKDRAPLLDKLREILKDYK
                     LELTRENVKQFLTEKAKELLSALKKKISSDPHAFQRGYSCDELKGMTDALAISLVENR
                     EEDITDNRIKIRLENQEEHWNVLCNEEDSERFLDSTPSRLKMTSLEAYRGDKQVSAPT
                     SEQLDLIEEPGVFLRERTIDNPGLGNCAFYAFAIGLVNIIQEEAKYNRRTMFDRWVGL
                     DRSISGQYDEILKLNLEDPDKELLDRLQSSLRIVTYQYQIRELRNVCVFRNGNYNRLT
                     GNSNFVNFAALYYGDPLDTDSRFNPFADSVPILIKMANIDRDSVHPGHENDVLVPLFL
                     DLLYGDTTNPADITLETEPKSDSPIITAMNNITQDFFWGTHLDLNYLAEAFEVNLHVL
                     RNNSPIQEFVDIPERHTLTLTNSNNTHWTTQITTARATTGMLLGRNPHLSGAKRTREQ
                     QRVNEEFPGKESANDFMIPPFDEISNPHHKKEVETNKPIGKGFFHDRPVVSEEQRKLE
                     QLKRIVANATLTYTTYSESIWFCFFHYHGQTGRDRANNFCKTFSKITDYGQAKEALVH
                     YLNDSRNGNTHPHSYRTMLLHELQGNHDNKKDLQYLSRHFTSSLNKLASVLEVQLFAN
                     RYVP"
     gene            complement(9432..10088)
                     /locus_tag="AC06IO_002275"
     CDS             complement(9432..10088)
                     /locus_tag="AC06IO_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIO9875450.1"
                     /translation="MQNIQHLIDYILHIDVYLNAFVSTYGFWTYLALFTVIFCETGLI
                     VTPFLPGDSLLFAAGSIAAQPGNPMNIMLLFVLLFMASILGNQVNFLVGRAIGPRVFS
                     AKRSWLLNPKHLHDTHAFYEKHGGKTIILARFIPIIRTFAPFIAGIGRMRSLHFTLFN
                     LISAILWIGSLLSLGYYLGSIPLIKENFSLVIYGIIAISILPPIIALISKKSTSSIKK
                     "
BASE COUNT         3145 a         1837 c         2301 g         3024 t
ORIGIN      
        1 tttgattatt gattcttaaa atgctattgg caaaacaatt cagatttgcc gggtttacat
       61 tcattttcat ttaatcaaaa gggaattgct atgaataaat tacaattaac aggcgctgct
      121 ttagctgttg gcgctgcttc tatgtttgct ttggctcctg catttgctgt tgatgctaca
      181 accacaacaa ttcactgtaa aggtgttaat gcttgtaaag gtcaaggtag ctgtaaaact
      241 gctgataatg cttgcaaagg tcaaaattct tgcaaaggca aaggtattct tgagatgtct
      301 aaagaagatt gcgaaaaaca aggtggtact gttgctgagt aatagcaatt agttcagtca
      361 catcttacca ggcatgtaga aaaacatgtc tggtccccat tcttttctag gtttaatatg
      421 gaagtatccc caaataagtc ggctcaaaaa ttaccttatt taggttttgg tttgggattg
      481 cgaccaaact attatgaaga aatcctgacg agcaaacctg atctggattg gtttgaaata
      541 ttaactgaaa attatttaat ccccggaggt aagcctctct attatctgga caaaattcgc
      601 gaacactatc ctattgtcat gcacggcgtc tctctttccc tgggaagtac tgatcctttg
      661 aattgggatt atttgaatca ggtacaagaa ttagcttctc gcattgagcc tgtttggatt
      721 tcagatcatt tatgctggac tggcgttcat ggattaaata cacatgattt attaccggtt
      781 ccctatacaa ctgaagctat tcagcacatc gtatcccgta ttcaagaaat acaggatttc
      841 ttaaaacgtc cttttttaat tgaaaatgta tcaagctacc ttacatacaa acagtcagaa
      901 atgtcagaat gggattttat tctggaaata gtaaaacaat ccggttgtta tttattgttg
      961 gatgtcaaca atgtttatgt cagttcattc aatcataatt tcgatccaat ggcctatatt
     1021 aattccatgc ctcctggaag agtagctcaa atccatttgg cgggtcacac taatcatgga
     1081 gattacatta tagacactca caatgctcca gtgattgagc cagtttggga tctttatgag
     1141 gcaaccatac agagacttgg tcctgtatct actatgattg aacgtgatga taacatgcct
     1201 gattttagcg agcttttaag tgaaattaat catgcaaaac gccttgccat acaagccgca
     1261 aaagaaaagg tagccgtatg aaatcgctat tgactttaca aacccaattt caagaattca
     1321 tttattccgg gcagggctca attgaagaat ccatagtgcc aaccaagtta gtttcaacag
     1381 aaatgcgtct gaatatttat aaagacggtt atcgattgag attgattgaa tgtttggcag
     1441 caaattaccc cgctttatat gcgtatttag gtactgagga atttgcaaag ttaagtgctc
     1501 gttatattga ttcttaccca tctgcttacc gttcaatccg atggtatggc gatagattgc
     1561 ctgaattgat aaaaaagtac tatgcgaagt cttgctatta tttgtatgaa ctggctgagc
     1621 tggagtggaa aatgacctta gctttcgatg cagcagatga tacagtgctt aatgtagaag
     1681 agatggcagc agtgccgccc gaatcctggg ctgagatggg gtttgtactt cattcgtcag
     1741 tccataggct tgatttttgt tggaatgttt tttcattgtg gcaagctttg gtcaataatc
     1801 ttgagattcc agagttgaaa gatagtcctg ctgctactgg ctgggtgtta tggcgggggc
     1861 aaaattatat cattcaatat tacagcttgt cggaagaaga ggcttgggct ttagacgctc
     1921 tgattcaggg ctcctctttt ggggtgattt gtgagggatt atgccagtgg atgcctgccg
     1981 aagaggttgg aatgaaagca gcgtcttatt taaaaaactg gattcaaaat gggttattgt
     2041 ctcagtgtcg tattcgccgc aattagacat tgggaggtaa agaagcttac attgacagaa
     2101 cgacataagg atatgccatg ctaaacccaa gagagatccg aaccatagaa gatgcaaaac
     2161 gcatcgtgga agaaagacaa ttaactcatg ttaaggttgg tctttttgat atcgatggtg
     2221 taatgctggg caagtacatg agccgtaaca agtttttttc ggctttggag catggttttg
     2281 ctttttgtga tgtcatttta ggatgggatt ctaaagataa gctttatgat aatgtgaaat
     2341 atactggctg gcatacgggt tatcctgacg cctcagtgcg aattattcct tcaacctgtc
     2401 gtgaaatatt atttgaagaa aatcaactgt tgtttatggc tgaattttca caagcagcag
     2461 aagccatctg tcctcgcggg atcttgcgtc gtgtaatcaa taaagctgcg ggcatggggt
     2521 atgatgttta tgccgcattg gaatatgaat tttttgtatt tgatgaaaca cctgacagcg
     2581 tgcgtgcaaa aggatttcgt aatctaaagc ctgttactcc tgataatttt ggctactcaa
     2641 ttattcgtaa tacggtacat gctgaatttt atcatcaaat tctcactatg gcggaaaaaa
     2701 tggatttccc cattgaaggg ttacatacgg aaacaggccc gggagttctg gaagcagcga
     2761 tagctgttga tgatgccgag gcagcaggag ataaagcggc tttgtttaaa acatttatga
     2821 aaattctcgc gcaacgcaat aataaaatgg caacttttat ggctaaatgg tcaggtgatt
     2881 accctgggca aagtgggcat atacatctgt ctttgcgaga tagagatggc ggcaagtccg
     2941 ctttttatga atcaggcaga gaatataata tgagcaaaat tcaacgccat tttttagccg
     3001 ggcagcaaaa gttgatgcct gaatttttag ccatgttatc cccgactgtg aatagctatt
     3061 ccagactaat tcccggctat tgggctccaa cagaggcaac atggggtgtc gagaaccgca
     3121 ccactgcttt acgagtgatt cccggcagtg ataagtcaca acgtattgaa tatcgactgg
     3181 gttctgcaga tgcaaaccca tatctcgcgt tggctgctgc catagggtcc ggtttatatg
     3241 gcattgagaa tgagttggag cctgaggctg aggtaaaagg gaattcctac gagcaacatc
     3301 atagtccggc attggcgttg cctcgcacat tatgggaatc ggcacaaaat ttaaaaaact
     3361 cttctgctgc ttatcaatta tttggtcaag aatttgttga gcattttgct gcttcaagag
     3421 aatgggaaga gagagaattt agaaaacata ttactgattg ggaactcgac aggtattttg
     3481 aaattattta attgaaacga cagattttat aaaacttatg gattaaaaat gattgctacc
     3541 ttaaaaacct attctcctgt cgataactct ctttatgttg agagagagta tgcgaatcaa
     3601 gaagagataa aaactgcttt aagcaaagca caagtagcaa aaaaactgtg gtctttgtct
     3661 tctctggccg aacgccagga atattgtttg gctgctgtca atgaaatagt cgctagcaaa
     3721 gtagatattg cagaagaaat ttgctggcaa atgggaagac caattcgata cgcagctggt
     3781 gaaatcaatg ggtttgaaga gagagcgaga tatatgattg cacaggccga atcatcactg
     3841 tcttctgtgg aattacctga aaaagaagga tttacaagat acatcaaacg agagccgtta
     3901 ggacttgtgt tggttatagc gccatggaat tatccctatc tcacagcagt caatgcgatt
     3961 attcccgcac ttatggcggg taatgttgtg attttgaagc attctgcgca aactccgtta
     4021 gtggctgagc gtttcgatca ggcattcaaa aaagcaggct tacccatggg tgtttttcaa
     4081 tatttgcatt tgacacatga agatacagaa caactcatga gatcgccagc agttaattct
     4141 gtcgctttca caggttcagt tgctggtggt gaaagagttg aacagacagt ggcaggacgt
     4201 tttattcctg tgaatttgga gctaggtgga aaagatccgg cttatatcag agcagacgct
     4261 gatttggacc atgcagtaga aacagtcata gatggtgcgt tttttaattc aggtcaatcc
     4321 tgttgtggta tagaaaggat ttatgttcat aaagagatat acgaacaatt tgttaataaa
     4381 gcggttaatt tggttaaaca gtataaattg ggacggcccg atgatccaga aaccactctg
     4441 ggtccactgg tacgtgcttc ttcagctgac tttgtcagag agcaaatcaa agaagcggta
     4501 gcccaaggag cggtagcaca tataaatacc cgggattttg taatggatag acctggtaca
     4561 ccctatctgg caccgcaaat tttaaccgat gttaatcatc gcatgcgaat tatgacagaa
     4621 gaaacttttg gcccagtcgt agggatcatg tcggttgata gtgatgaaga ggcaatcgaa
     4681 aggatgaatg atagtgacta tggtttgacc gctgctgtat ttactcagga tattcatcga
     4741 ggaatagcga ttggtgagcg attacaaaca ggtacttttt ttatcaatcg ttgcgactac
     4801 cttgatcccg gtttggcttg gacaggtgta aaaaaatcag gcaggggttg cactttatcc
     4861 gccataggct atgagtattt aacgcggccc aaatcattcc atatcagaac taaaattgag
     4921 acttaaagcc tgatacctac gattacttac gtcttaaaac ccaaaataaa attataaaaa
     4981 agacctaatc tttcctgcgg aaaggtactt gctatcagtt acaatggtct ttttcgaatc
     5041 aaaggaagat catagatgaa aaaccaatca ttaattgtca attggaatta cccgacacga
     5101 atcctggttg gggctgggcg aatcaatgaa ctagcgaaag tatgtcgtga cttaggtatg
     5161 aatgctcctc tgttagtcac agacccggga ttggcttcgt cgctaatggt gagtaaagtc
     5221 atacaacagg ctcaggcatc cggtttgcga acggggttat tttgccaaat taaaacaaat
     5281 cctaccggtg acaatgtgat gaatggagtc tatgctttta aagctggcca gcatgatggc
     5341 gtgattgctt tcggaggtgg ctcggctttg gatgccggca aagccatcgc tttaatggtt
     5401 ggccaggatc gacctttatg ggatttcgag gacgtgggtg ataactggac tcgagtgaat
     5461 gtttcagcca tggccccagt tcttgccatt ccaacaacag caggaactgg ttcagaggtc
     5521 gggcgagctt ctgtaattac cgatgctgaa aagcaggtta aaaaaatcat ttttcatcct
     5581 aaaatgttgc ctgccatggt tattcttgat ccagagttaa ctgtcgggct tcccaaatca
     5641 cttactgcag ctacaggtat ggatgctctt tcccattgtc ttgaagctta ttgttccaat
     5701 tattatcatc ctatggcaga aggtattgcg ttggaaggaa taagattaat caaggagaat
     5761 ttaattcaag cttacaagga tggtagtgac ctggaggcaa gaacccatat gctggttgct
     5821 tcagcaatgg gagctaccgc ctttcagcgt ggtcttggag cgatgcacgc cttggctcat
     5881 ccattaggtg caatttatga tgtccatcac gggcggctta atgcggtttt gatgccttat
     5941 gttttattgg ctaatcgtag tgaaattgaa aacaagatag agcgtctggc aaattattta
     6001 tcaatagcaa atggatttga tggttttttg gattggatag agcagatgag attagagtta
     6061 ggaatcgaaa atacattaag ccagttaggt attgatcatt cgcagataga cagactggca
     6121 aaaatggcca ctgaggatgc ggcagcagct tccaatcctg ttctttttac attagagcaa
     6181 tacaaagaac tattgagcaa agccattggt gagtgatcgt tgtaaaggaa aaggtgcaga
     6241 aacgggttgt ttaccaggta taaatcagat ggaaacgact tgtatattga aattaagtct
     6301 aatccagaca ataaaaattt tgcgataatt aattaggtga attaaatgaa tcttggtatt
     6361 ttgctttgtg ataaggtaag tgaagtattt gttgcggatc atggtcaata ccctgagatg
     6421 tttgccaatc tattgcgccc ggcggattct actcttgaat ttactgtatt tgatgcagag
     6481 catggggagc tgccaacaga tatccatgcc gtagacgctt atctgatatc aggaagtcga
     6541 catggggtga atgatgatta tccctggata agaaagcttg aagagtttgt tcgtacttta
     6601 catgcttccc aaaaaaaatt aattggtatt tgctttgggc atcaattaat agcgaaagcc
     6661 ttaggtggaa aagtgattaa atctcccaag ggttgggggg taggtatgtc acaaaatcaa
     6721 atttaccaat tcaaagaatg gatgagacca tctctaaatt gcttcaactt gctagttagc
     6781 catcaagatc aggtgattga gttaccaacc ggtgcggaga tattggcagg gagtgatttt
     6841 tgtccaaatt atatgatgca agttggttct ttctttagtg tgcaggggca ccctgagttt
     6901 actaaatctt attctcgtga tttgatggtg tctcgcgagg attcggtcaa tgaaattgaa
     6961 tttgcaaaag ggatgaaatc tcttgagtta catgagaatg acacaattat tgcccagtgg
     7021 atagttaatt ttttaaaagc ctgatgagct gtttttaatt gattattata cccccttaga
     7081 gtacaatatg tgaacattaa ttttattcac tgaccagaac tatcgggagt atgtatggcg
     7141 aagacaatta aggctactgg agatggcgct tgtctattta atgcagtgtc aatcggactg
     7201 agtgtcgaga tcttgagcgg aagactggat tcgcagctgg atacacccgg ttatcaggcc
     7261 ttgctggatg aatttgccaa acatcacccg caatttaacc ccaaaagctg gaaaacttta
     7321 aaagagtggc tagcctatta caacgatacc agagacattg agctgatttt agcacccgta
     7381 ttatttaatt tgaatcagaa atatcaagat catctggatg aagaaattct aaatgaatta
     7441 accaatcttg tatggaaaaa taaggccaat attgaaaatg gccaagcctg gtttcaattg
     7501 caaaacacag gggatttggg cgaggcactt tttcccaaat tggaaaacct ggatttgaaa
     7561 aaagatagag ctcctttatt agataaatta cgagaaattc ttaaagacta caaacttgaa
     7621 ctgacacgtg aaaatgtaaa acaatttctg accgaaaagg ctaaagaatt attgtccgct
     7681 ttaaagaaaa agatcagttc agaccctcat gcctttcaaa ggggttatag ctgtgatgag
     7741 ttaaagggta tgacagatgc cttagcgatc agtctggttg agaatagaga agaggacatt
     7801 acggacaacc gaatcaaaat cagattagag aaccaggaag agcattggaa tgtattgtgt
     7861 aatgaagaag atagcgagcg ttttctggat tcgacaccct cacgtttgaa aatgacttcc
     7921 ttagaggctt atcgaggtga taaacaagtt agtgcaccaa catctgagca gttggatttg
     7981 attgaagagc ctggcgtttt tttaagagaa agaaccattg ataaccctgg tcttggcaat
     8041 tgcgcttttt atgcctttgc gataggtttg gtgaatataa ttcaggaaga ggcaaagtat
     8101 aatagaagaa ccatgtttga tcgatgggta ggtctcgaca ggtcaatttc aggtcaatac
     8161 gatgagattt tgaaacttaa tcttgaagat cccgataaag aattactgga cagattgcaa
     8221 agctccttaa gaatagtgac ctatcaatat caaatacgag aattaagaaa tgtctgtgta
     8281 ttcagaaatg gaaattataa tcgattaact ggaaacagca attttgttaa ttttgcagcc
     8341 ttatattatg gcgatcctct ggatacagat tctcgattca atccttttgc cgattctgtt
     8401 cctatactga ttaaaatggc aaacattgat cgagacagtg ttcatccagg acatgaaaac
     8461 gatgtattgg ttcctctctt tttagattta ttatatggtg atactacgaa tccagctgat
     8521 atcaccctgg aaacagaacc aaagagtgat tcacctatca ttactgcgat gaataacatc
     8581 actcaggatt ttttctgggg cacccatttg gatttaaatt atttagccga agcgtttgag
     8641 gtcaatcttc atgtgctaag gaataatagt ccaattcaag agtttgtcga tattcccgag
     8701 cgacacactc ttactttaac taatagcaat aatacgcatt ggactaccca aattacaaca
     8761 gcgagagcca ctacaggaat gcttttgggg cgaaaccctc acctgagtgg agctaaaaga
     8821 acccgggagc aacagcgagt taacgaggag tttcctggaa aagaatcagc caacgatttt
     8881 atgatacctc cttttgatga aatatctaac cctcatcata agaaagaggt agagaccaat
     8941 aaaccaatcg gtaaagggtt tttccatgat agaccggtag ttagtgaaga gcagagaaaa
     9001 ttggagcaat taaaacgaat tgttgccaat gctacgctca cttacactac ctacagtgaa
     9061 agcatctggt tttgcttctt tcattatcat ggtcaaacag gccgagatcg tgctaataac
     9121 ttctgcaaaa cattttctaa aataacagat tatggccaag ccaaagaagc tttggttcat
     9181 tatttaaatg acagcaggaa tggaaataca catccacatt cctacagaac aatgttactg
     9241 catgaattac aagggaatca tgacaataaa aaagatttgc aatatttgtc caggcatttt
     9301 acttcttctt taaataaact agcatctgtt ttagaagtgc aattgtttgc caacagatat
     9361 gttccttagt aagagcgtgg tgtaaaaggc atcctagagc cctttgcact cgcataagtc
     9421 cctaaagtat attatttttt aattgatgac gtcgattttt tactaattaa agcgatgatg
     9481 ggcggtaaaa ttgaaatcgc tatgatccca tagataacta atgagaaatt ctcttttatt
     9541 aatgggattg agcctaaata atatcccagg ctaagaagac ttccaatcca aagtatggcg
     9601 ctgatcagat tgaaaagcgt gaaatgcaat gatcgcatcc tgccaatacc tgcaataaat
     9661 ggagcaaagg tgcgtattat gggaataaaa cgcgccagta ttattgtttt gccgccatgc
     9721 ttctcataaa aagcatgggt atcatgcaga tgcttgggat tcagtaacca ggagcgtttg
     9781 gcgctaaaga cacgagggcc tattgcgcgt ccaacaagga aattcacttg attccctaat
     9841 atcgaagcca tgaaaagcaa aacaaacaac agcatgatat tcatagggtt tcctggttga
     9901 gctgctatac ttcctgcagc aaataacagg gagtctcctg gtaagaaagg agtgacgata
     9961 aggcctgtct cacaaaagat aacagtaaat aacgcaagat aagtccaaaa cccatatgtt
    10021 gatacaaagg catttaaata aacatcgata tgaagaatgt agtcaatcag gtgctgtatg
    10081 ttttgcatag tgtctctttg gattaaatgt atgacgtaat tccatgtgtc tatactaact
    10141 tgagttatgg ataaaatcag ttgttatctc aaaccgctcg gctgagaggg catttatatg
    10201 ccgtctcgaa gctttgtacg agatgtaata ttttatacca aggcttcaag acgatgctta
    10261 tgcatctcct cagcctgaac ggctccctgg gtatcgattg gttctta
//
LOCUS       DBNCWK010000111        10305 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.111, whole genome shotgun sequence.
ACCESSION   DBNCWK010000111 DBNCWK010000000
VERSION     DBNCWK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.111"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            198..294
                     /gene="ffs"
                     /locus_tag="AC06IO_002276"
     ncRNA           198..294
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC06IO_002276"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            320..1990
                     /gene="dnaX"
                     /locus_tag="AC06IO_002277"
     CDS             320..1990
                     /gene="dnaX"
                     /locus_tag="AC06IO_002277"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIO9875451.1"
                     /translation="MSYLALARKWRPRTFSQLVGQEHINKALINALNQQRLHHAYLFT
                     GTRGVGKTSVARILAKALNCEKGITSEPCLHCDTCIAIEQGRYIDLIEIDGASKTRVE
                     DTRDLLDNIQYAPTNGRFKIYLIDEVHMLSQHSFNALLKTLEEPPSHVKFILATTDPQ
                     KLPVTVLSRCLQFNLKHLPAELINQQLQLILQEERLDFEIQALDILARAARGSMRDAL
                     SLLDQAITSCETKLLANDVKMLLGHTQQDYALLLLQALAKQEAPIMLALSQQIVAEGG
                     NFQYVLDEILNYLHQITVYQCLGDNNPLISPKTEIKELAKQFSAEDTQLFYQIGIKGR
                     EEMHLAPTLTIGFNMTMLRMYTFKPASHVTTPPLAFEQNNKTCSLIVDDNTPAQNPIG
                     IEPIPVTKATDSTLAYHQTPSLSTDDKKSANAGESMSRDWNDIIPQLKLSGLALNAIE
                     NAEFLGKKEREITLGVQKGHQSLFTPTTINRIETALAGYYKSPVKIILNSQDAVHASP
                     AQQKEKATQERQEQAEAALHADPVFQQLKQEFSAELVKNSIVLLKDDL"
     gene            2024..2362
                     /locus_tag="AC06IO_002278"
     CDS             2024..2362
                     /locus_tag="AC06IO_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIO9875452.1"
                     /translation="MDINQNLGNLMKEAQKMQQRMQEAQQQLSQLVVSGESGGGMVTI
                     KMNGRHDVTEVKIKPTLMDEDIEMLEDLIAAAVNDAVRKIEKASKEKISQLTAGLNIP
                     TDLMGGKEGE"
     gene            2371..2964
                     /gene="recR"
                     /locus_tag="AC06IO_002279"
     CDS             2371..2964
                     /gene="recR"
                     /locus_tag="AC06IO_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIO9875453.1"
                     /translation="MDALSRLVEALRCLPGVGPKSAQRMVFHLLQHQRQRGLHLASCL
                     EQAMKHISHCQQCNNYTEQTLCTLCQNPNRDSTLLCVVESPADVSAIEQSNSFQGKYF
                     VLMGKISPLDGLGPDDIGLPKLKELIIREKIQEVILALSPSVESQTTIHFIHQLLKDE
                     AVNISQLAHGIPSGGELEFLDGNTISSALKNRAVINV"
     gene            2957..3817
                     /locus_tag="AC06IO_002280"
     CDS             2957..3817
                     /locus_tag="AC06IO_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIO9875454.1"
                     /translation="MSKSIFILCRKKILVLLLLSLCFITSTASASFHRSLWPKWLVNA
                     PLSKQSISHQAWQHFLDHHVITNEEDINLVDYTNINEKELASLKEYIKNLSQIDIDNY
                     NRQEQLAYWINLYNALTVLTVANYYPIANIQEINISPGLFSVGPWGANIITIKNTNLS
                     LDDINNRIIRPIWNDPRTHYALNNATIGAPNLSKQAYQGPLLEQQLNDAAFKYINSLR
                     GVHVIEGKLIVSKLYDWYEEDFGGTKKYVIKHLLQFAKEPLRNQLKHINTIDSYIYNW
                     HINCPADNQS"
     gene            3814..5811
                     /locus_tag="AC06IO_002281"
     CDS             3814..5811
                     /locus_tag="AC06IO_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIO9875455.1"
                     /translation="MKKKHLIFAYFSQFLLFNLCFFGLQLLLLYSHAESLISSIKLPL
                     PIYFEIIGTLVIQFSLYIIISTIQALLLIGILNRSWHYFSTEQWQTIIWSLFACAILS
                     ANAYYFPLSVFSKLFSPPIPEFIFLILLYFSLACLGLLLLNCLFYRTTARLLSIALPI
                     GICVFLLNNYWKSSLNPNVDSEPNVIILGIDSLSPESVTSRNMPFLHQMLLDSFQFTN
                     TISPLARTYPAWSSILTGLYPKHHLAEENLVERSRVKSQLSIVWDLNKLGYNTIYATD
                     DRRFNSIDENFGFKKIIGPKTGVNDVMLGSYNDFPLGNLLVNFRISAWLFPYNYSNRA
                     SFISYYPETFTNELKRELSLQQNSPVFLAVHFALPHWPYAWAASLPDQVENEFSLSKR
                     DALYLRALKAVDNQFKVIFCYLKKHGYFKNSLVIILSDHGEVLYYPNSRLTSYQKYQS
                     SLTSKLAEYFKAKTATELDKSAGHGSDILSPMQYHSLLAFNVYKDGKLLTKTNKIKTR
                     VALFDLAPTILDFLNIAKKQKMDGISLLPTVLNPNTPLPHRIFFIESGMYPNQDFTKE
                     KAIELGKKIYKVNPDTGQLELKPDELIQVNNQKLYGVISGNWILALYPDDNTYIPVIL
                     NLSTGEWIDNLQSDFAKSTPAEKLKRQLQEFYGKKLAYPIQ"
     gene            complement(6038..7516)
                     /locus_tag="AC06IO_002282"
     CDS             complement(6038..7516)
                     /locus_tag="AC06IO_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875456.1"
                     /translation="MTQKTLTLPNWFVTDNDRAKARIKYELKQLETQLGKCVKQPDDT
                     AFSKNKHSLYMALADKYSEYLKLIDIDSKFKQFVGVEIPKDISYPEACNRIMHLKQKR
                     SAAVNLLEEAKIIIEISSLYQYLAKAIEPNEVSDVSNHLILNQFDKLKQLFKKENLEL
                     WLSTLVDLWNHSSTLLREPAKLFRNWDLEEQTIALCFFSESEVVNLVNAIFFYKLYPD
                     KLFNELIHPEKLVSVRMRLGFLHEFIELLQKQLHSNALQYGLNPGIDFLFHGEELPQG
                     IMIEVNEVYRDIIQLAVKNLRVQFTPENNEKVTLERLHDLGRAYKFWFNPNRLIDAVM
                     VLQQRLVTESISQEEDLEKFHQEMLVLYRQLTTTECLDLYGYFANNDSRYLLYTLYSI
                     NNGYSLDWLPSLNSSERNAIARVYQALKCVMEALRVELKNRFITTEPYLYDLGKKEIH
                     TGRRNREAVFRVIAIYGCERIVMSDSLERLFNLIEEPNQQFE"
     gene            7739..8464
                     /locus_tag="AC06IO_002283"
     CDS             7739..8464
                     /locus_tag="AC06IO_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIO9875457.1"
                     /translation="MSQRHYLLFIDNEPVSEELKDYFEKFNLDIIQQKKLFPIHVEKG
                     LPTAILINWSILRTEPEAIEQFYNMYPVPLIVINNKPDEESSVAMLEAGADDFMTKPI
                     YPRELHARIGAISRRVIRAQQKVDHGKEVLMFANWRLYPASRQVFGENNEELQLSAGE
                     YDLLLTFVQQPQRVLDREFLLQITKNTDLVPFDRRIDVQISRLRQKIEIDKKKPALIK
                     TIRNGGYMFTARVVTLKESDATL"
     gene            complement(8689..9330)
                     /locus_tag="AC06IO_002284"
     CDS             complement(8689..9330)
                     /locus_tag="AC06IO_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875458.1"
                     /translation="MKEQFFQTKNLNTLLQSVEKPIQDLLDLNFKTLKTVSYVTPIEL
                     FNVLKPEEILEKNMNVFIQNSQKAMSYMLNVFQIMEHHWINIYDQMAENPNKKKANPA
                     IDKANRKVDTNTPKSSASKKAVTAKGKAKPAALQSKSKTSKAKPKISSPTKVKTNTQE
                     RPAAVKQQSQMPLSSLQKDTEKQGINTPVALKNSVAILKNETKDIVPSNNKLK"
     gene            complement(9609..>10305)
                     /locus_tag="AC06IO_002285"
     CDS             complement(9609..>10305)
                     /locus_tag="AC06IO_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIO9875459.1"
                     /translation="FSRHQAGDLKSMVELWKKNDERFSPERIEIIYFNDDINYDQLDE
                     KITIYICAHGSDDENLTKLFNHSNPLVAESIDIKEVAERFERDILPISYWISAVHLYC
                     CGSKNKNQMMAELLGHSLLRPEKPIYHYSGSVSIVDEYGKQWSFANHVKIPVHLTAKR
                     IFVLNFFDEEQPHRAFVKKAFQSKTYKELLAKKEDSFFAKVKENRASVLLSKRLGKKS
                     RDGEENSLLRKGN"
BASE COUNT         3289 a         2029 c         1757 g         3230 t
ORIGIN      
        1 ttcctgatat tttgtagctt aaaaggaaat atttattcat gtggattgga tatgctcttt
       61 attagcccag gttatttaat tgctttttta atttgtattc tttaggaaag taggctcact
      121 caaataataa aatagtcatt agctctagcg caggaatatt ttttttgtta ctcttcgcta
      181 ttcaaatatg gtgactctat tggtcccctc gcgacgatag attgtgaacc ccgtcaggcc
      241 cggaagggag cagcggtagc agttgatgcg ggcgccgggg tgtggctctt agagtcgccg
      301 cccaacttgt gaaacacgta tgagctatct ggcacttgcc cgtaaatggc gaccacgcac
      361 attttcccaa ctggttggcc aagagcatat taataaagca ctgatcaacg cgctgaatca
      421 acaacgtttg catcatgctt atctatttac tggtactcgc ggggtcggga aaaccagcgt
      481 cgcccgtatt ttggctaaag ccctcaattg tgaaaaaggc atcacctcag aaccatgcct
      541 tcattgcgat acctgtattg ccattgagca aggccgttat attgatctca ttgaaataga
      601 cggagcctcc aaaaccagag ttgaagacac gcgagattta ctcgacaaca tacaatatgc
      661 accgacaaat ggccgtttta aaatttatct aattgatgaa gtgcatatgc tatcccaaca
      721 tagctttaat gctttactaa aaacattaga agaaccgcct tcacatgtga aatttattct
      781 ggcaacgact gacccacaaa aattacctgt tactgtctta tcgcgttgtt tgcaatttaa
      841 tctaaaacac ttgcctgctg agttgataaa tcaacaattg caattaattc tccaagagga
      901 gcgcttggat tttgaaatac aagcgctaga catattagct cgggctgccc gcggaagcat
      961 gcgcgatgca ttaagtttat tggatcaggc cattaccagt tgtgaaacga aattactggc
     1021 aaatgatgta aaaatgctct tgggacatac ccaacaagat tacgcgttgc tgctcttgca
     1081 agccctggcc aagcaagaag cgccaatcat gcttgcatta agtcagcaaa tcgttgccga
     1141 aggggggaat tttcaatacg ttcttgatga aatacttaac tatctgcatc aaattaccgt
     1201 atatcaatgc ctgggcgaca acaatccatt aattagccca aaaacagaaa ttaaagaatt
     1261 agcaaaacag ttttcggccg aggacacaca attattctat caaataggta ttaagggacg
     1321 tgaggagatg catctcgccc ctacactgac cattggcttt aatatgacca tgttgcgcat
     1381 gtataccttt aaaccagctt ctcatgtaac aacacctcca ttagcattcg agcaaaataa
     1441 taaaacatgc tcactaattg ttgatgataa tacaccagcg caaaacccta ttggaattga
     1501 accaataccc gttacaaaag cgacggacag cacccttgcc tatcatcaga ccccatcatt
     1561 atcaacagac gataaaaaat ctgctaacgc tggtgagtcc atgagccgtg actggaacga
     1621 cataattcca cagttaaaat tatcgggttt agcacttaat gccatagaaa atgcagaatt
     1681 tctaggtaaa aaagagcgag aaattacatt aggagtacag aaagggcatc agtccttatt
     1741 cactcccaca acgattaacc gaattgaaac cgcattggct ggttattata aaagtcccgt
     1801 aaaaattatt ttaaacagtc aagatgccgt acatgcctct ccagctcaac agaaagagaa
     1861 agcgactcaa gaaaggcaag aacaagcaga ggcagcatta cacgctgacc cggtatttca
     1921 acagttaaaa caagaatttt cagcagaatt ggtcaaaaat tctattgtcc tgttaaaaga
     1981 tgacttataa tacttaaact aaccataaat agacgaggct gtaatggata ttaatcaaaa
     2041 tttaggcaat ttaatgaaag aagcgcaaaa aatgcaacaa cgtatgcaag aagcgcagca
     2101 acaattaagc cagttggtag taagtggaga atcaggtggt gggatggtta ctattaagat
     2161 gaatggacgt catgatgtaa ccgaagttaa aatcaaaccc accttaatgg atgaagatat
     2221 tgaaatgctg gaagatttaa ttgctgctgc agtcaatgat gcagtgcgca aaatcgaaaa
     2281 agcatccaaa gaaaaaatca gccaactgac tgcaggtctt aatattccaa ctgatttaat
     2341 gggtggtaaa gaaggagagt aaccctggct atggatgctc taagccgctt ggtagaggcg
     2401 cttcgctgtc ttcctggtgt gggaccaaaa tcagcacaaa gaatggtgtt ccatttattg
     2461 cagcaccaaa gacagcgagg tttacacctt gcctcctgtt tggaacaggc aatgaagcac
     2521 attagtcatt gccagcagtg taataattac accgaacaaa ctctgtgtac tctttgccaa
     2581 aaccccaatc gagattcaac attactttgc gtggtagaga gtccagcgga tgtttctgcc
     2641 atagaacaaa gcaattcctt tcaaggtaaa tattttgtcc ttatgggaaa aatttctcct
     2701 cttgatggct tgggacctga tgacataggt ctaccgaaac ttaaggagtt aattattagg
     2761 gaaaaaattc aggaagttat tcttgcatta agccccagtg ttgaaagcca gaccacaatt
     2821 cattttattc atcagttgct aaaggatgaa gccgtcaata tcagtcagtt agcacatgga
     2881 ataccatcag gtggtgaatt agaattttta gatgggaata ctataagcag tgcccttaag
     2941 aacagagctg tgattaatgt ctaaatccat atttatttta tgcagaaaaa aaatactggt
     3001 cttactcctg ttatccttat gtttcataac aagtacagct tctgcctcct tccacagaag
     3061 tttatggccc aaatggttgg tcaatgcccc tctttccaaa caatcaattt ctcatcaggc
     3121 atggcaacat tttcttgatc atcatgtaat aactaatgaa gaagacatca atctggtaga
     3181 ctataccaat atcaatgaga aagagctggc ttcactaaaa gaatatataa aaaatttatc
     3241 gcaaatcgat attgataatt ataaccgtca agagcaattg gcttattgga tcaatttgta
     3301 taatgccctt acagtattaa cagtagccaa ctattacccc attgcaaaca ttcaggaaat
     3361 taacatttct cctggattat ttagcgttgg gccttgggga gccaatatta tcacaatcaa
     3421 aaatactaac ttatccctgg atgatatcaa taatagaatt attcgcccaa tttggaatga
     3481 cccaagaact cattatgctt taaataatgc caccatagga gcgcctaatc tgagcaaaca
     3541 agcataccaa gggccattgc tggagcaaca acttaacgac gcggcattca aatacattaa
     3601 ctctctcagg ggagttcatg tgattgaagg taaattaatt gtttctaaat tatatgactg
     3661 gtatgaagaa gattttggtg gtaccaaaaa atatgtcatc aaacatttgc tacaattcgc
     3721 gaaagaacct ctgcgtaacc aattaaagca tattaatact attgatagtt atatttataa
     3781 ctggcatatt aattgccctg cagacaatca atcatgaaaa aaaagcattt gatttttgca
     3841 tattttagcc aattcctttt atttaatctt tgcttttttg gcttgcaatt gcttctgctc
     3901 tattcgcatg ctgagagcct aatcagttct attaagcttc ctttgccaat ctattttgaa
     3961 attatcggca ctctcgtaat tcaattcagc ctgtatatca tcatttccac gattcaagct
     4021 ctgttgctta taggtatttt aaatcgatct tggcattatt tttctactga gcaatggcaa
     4081 accattattt ggtctttatt tgcatgtgct atattaagtg ctaatgccta ttatttccct
     4141 ctgagtgttt ttagcaaact attttcacca ccaattcctg aatttatttt tttaatatta
     4201 ctttattttt ctttggcttg cctggggtta ctcttattaa attgcttgtt ttaccgcacg
     4261 actgctcgcc tattatcaat agcgctaccc attggcattt gtgttttcct attaaataat
     4321 tattggaaat cttccttaaa tcctaatgtt gattccgaac ccaacgtcat tattctgggc
     4381 atagacagct taagtccaga aagtgtcaca tctagaaaca tgccttttct tcatcaaatg
     4441 ctactcgaca gttttcaatt tacaaacacg attagtcctt tagccagaac ctatcccgct
     4501 tggagctcca ttttaactgg gttataccct aaacatcatc ttgccgaaga aaatttagtt
     4561 gagcgaagcc gggttaaaag tcaactcagc attgtgtggg atttgaataa attggggtat
     4621 aacacaatct atgcgactga tgatagacga tttaatagca tagatgagaa ttttggattt
     4681 aaaaaaatca tcggtccaaa aacaggtgtc aatgatgtca tgttaggttc ttataatgat
     4741 tttcctcttg ggaatttact ggttaatttt cgcattagtg cctggttatt cccctataac
     4801 tatagtaaca gagccagctt catatcctac tatccggaaa cctttacaaa tgaattaaaa
     4861 agagaactgt ctttacaaca aaattcccct gttttccttg cggtgcattt cgctcttcca
     4921 cattggcctt atgcctgggc agcatcattg cctgatcaag tggagaatga attcagcctc
     4981 agcaagcgtg atgcgcttta cctaagagcg ctaaaagcag tagacaatca atttaaagtc
     5041 attttctgct atttaaaaaa acatggctat tttaaaaata gcctggtcat tatactaagc
     5101 gaccatggcg aagtacttta ttatcccaac tcacgattga caagttacca aaaatatcaa
     5161 agctcactaa ctagcaaact cgcagagtat tttaaggcca aaactgcaac tgaactggat
     5221 aaaagtgctg gccatggttc tgatatatta agccctatgc aatatcatag ccttctggca
     5281 tttaatgttt ataaagatgg aaagctactt acaaaaacaa acaaaatcaa aacaagagta
     5341 gcattatttg atttggcccc aaccattctg gacttcctga atattgcaaa aaaacagaaa
     5401 atggatggca tttcattact acctactgta ttaaacccaa atactccact accacaccgc
     5461 atattcttta ttgaaagtgg aatgtatcca aatcaagatt ttactaaaga aaaagccatt
     5521 gaattaggaa aaaaaatcta taaggttaat ccggataccg ggcaacttga gttgaagcct
     5581 gatgaattaa tccaggtgaa taatcaaaag ctatacggag tcattagtgg aaactggatt
     5641 ttagccctat atccagatga caacacctac atccccgtta ttctcaatct ttcaactgga
     5701 gaatggattg ataacctgca gagtgatttt gccaaatcta ctccggcaga aaaactaaag
     5761 agacaacttc aagaattcta tggtaaaaaa ctggcttatc ccattcaata aaagcgcttt
     5821 ataaaactca ctgcaaagcc cccatataaa gcagcagacc tcttttattt tttgcttcag
     5881 agcatggcag aagcagagtt tatatgcagt agatgagtgc cacagaaacg tttacaatgc
     5941 agcaataaaa tgaaagaagc aagttataaa agaggttggg tatatcccaa aaaacgaatt
     6001 actcttagtc aattggcacc tgaaaaattc acatttacta ctcaaattgc tgatttggtt
     6061 cttcaatgag attaaacaat ctttctaatg agtcactcat cactattctt tcacaaccat
     6121 aaattgcaat gactctaaat acagcctctc gattacgtct tcctgtatga atttcctttt
     6181 tccccaaatc atatagataa ggttcggttg tgataaatct gttttttaat tcgactctta
     6241 gcgcctccat aacgcacttc aacgcttgat acactctggc tatagcattt ctttcagatg
     6301 aatttaatga tggcaaccaa tctaaagagt aaccattatt tattgaatat aaagtataaa
     6361 gtaaatatcg actgtcattg ttggcaaaat aaccgtataa atccaggcat tctgttgtgg
     6421 tgagctgacg atataaaact agcatttctt gatgaaattt ttccaaatcc tcctcttgag
     6481 atatactttc cgttaccaga cgttgctgca gaaccatcac agcgtcaatt agcctgttag
     6541 gattaaacca aaatttgtat gcccttccca aatcatgcaa acgttccagg gtaactttct
     6601 cattattttc aggtgtaaat tgcactctaa ggtttttaac agctaattgg attatatctc
     6661 tataaacctc attcacttcg atcataatgc cttggggtag ttcttcacca tgaaatagga
     6721 aatcaatacc tggatttagc ccatattgaa gcgcattgct atgtaattgt ttttgtagaa
     6781 gttctataaa ttcatgcaaa aaaccaaggc gcatacgtac tgaaaccaat ttttcaggat
     6841 gaattaattc attaaataac ttatcgggat acaatttata aaaaaatata gcatttacta
     6901 aattaacaac ttcggattca gaaaaaaaac ataaggcgat cgtttgttct tcaagatccc
     6961 aattgcgaaa taacttggct ggctccctta ataaagtaga actgtgattc caaagatcaa
     7021 ccagagttga taaccataat tcaagattct ctttcttaaa taactgcttt agtttatcaa
     7081 actgattcag gattaaatga ttgcttacgt ctgaaacctc atttggctca attgctttag
     7141 caagatattg atacaatgat gatatctcaa tgataatttt agcttcctcc agcaaattca
     7201 ctgctgcaga ccttttctgt ttcagatgca taatccgatt gcaagcttcc ggataactaa
     7261 tatctttagg tatctccacc cccacgaatt gtttaaattt agaatcaata tctattaact
     7321 tcaaatactc cgaatattta tccgccaaag ccatgtaaag tgagtgcttg ttctttgaaa
     7381 aggcagtatc atccggctgc ttaacgcatt tgccaagctg agtctccagc tgtttcaact
     7441 catatttgat tcgcgccttg gctctgtcat tatccgtaac gaaccaattt ggtaaagtaa
     7501 gtgtcttttg agtcatccct ttctcctcaa gcattatcct gacacttgaa ctttaacaat
     7561 agcattgaaa agaaatattt tcctaatttt tataacgaac aaaatgcaaa ttttgcaata
     7621 aaccaggttt tgcgctctaa aaggctctgg caagcataaa tatttaaaaa taattgcaag
     7681 caactgataa acttgccaaa attaaagcaa tatatttttt gacctttgag gcaaattgat
     7741 gtcacaacgc cactatttgc tatttattga taatgagcct gtaagtgaag aactgaaaga
     7801 ttactttgaa aagtttaatc ttgatattat tcaacaaaag aaattgtttc ctattcatgt
     7861 tgaaaaaggg ttacctacag ctattttaat taattggtca attttaagaa ctgaaccaga
     7921 agcaattgaa caattttata atatgtatcc agtgccatta attgttatta ataataaacc
     7981 tgatgaagaa tcaagcgttg ccatgcttga agccggtgct gatgatttta tgaccaaacc
     8041 catttatccc cgtgagctgc atgcgcgaat tggtgctatt tctcgtcgag taatcagagc
     8101 gcaacaaaaa gtagaccatg gtaaagaagt attaatgttt gctaactggc gactttatcc
     8161 agcttccaga caagtgttcg gggagaataa tgaagaattg caattaagcg ccggtgagta
     8221 tgatttgctt cttacttttg tccaacaacc gcaacgagtc ctggatagag aatttttatt
     8281 gcaaattacc aaaaatacag acctggttcc ttttgaccga aggattgatg tccaaattag
     8341 tcgtttgcgt caaaaaatcg aaatagataa aaaaaagcca gctctaatca aaaccattcg
     8401 caatggcggc tacatgttca cagctcgagt tgtaaccctt aaagaaagtg acgccacatt
     8461 ataattgatt attatctggc ttatttagac aaattcgttc ctcttattta tcgagcgcat
     8521 tttaaagact ctgaactgat atttacttta ttggcacaat gcccgcctta tcctttactt
     8581 gctggcattg caccctatca tgtctagctt tggtatcccg gatttatcaa gtctcgtttg
     8641 ccacttctca aaaaagcgct caaaacgaaa acaggataaa gcaccagttt atttaagttt
     8701 gttattagaa ggcactatat cctttgtttc attttttaat atcgcaactg agtttttcaa
     8761 tgcaacaggc gtgttaatac cctgtttttc cgtatctttc tgcaaagaag ataagggcat
     8821 ttgcgattgt tgtttgacag ctgcaggccg ctcttgagta tttgttttaa cttttgttgg
     8881 actgctgatt ttcggtttcg ctttacttgt ttttgatttt gactgtaatg cagcaggctt
     8941 tgctttacct tttgctgtaa cggctttttt actggctgat gattttggag tattggtatc
     9001 tactttgcga tttgccttgt ctattgctgg attagccttc tttttatttg gattttcggc
     9061 catctgatca tagatattaa tccaatgatg ttccattatt tgaaatacgt tcaacatgta
     9121 actcattgct ttttgactat tttgaatgaa tacattcata tttttttcca ggatttcttc
     9181 tggttttaaa acattgaata attcaatagg agtaacataa gacaccgttt ttaatgtttt
     9241 aaaattgaga tctaacaaat cctgaattgg cttttcaaca gattgaagca aagtatttaa
     9301 attttttgtc tgaaaaaatt gctctttcat tacactctcc atatgttgca atgcacaaaa
     9361 acaagattag cttaaaaaaa taatttgtca aatattaaat gctattttct tgtaactcca
     9421 taaaccagta ccatcgctaa ctggcttcct tttaaatcat ttttagcctc ttattataag
     9481 tcttggctac aacataatca tatcaaaata cttactttta ttctgagttc aatgtttatc
     9541 gcacattcac agaatgtctt ttggtataac gaattgtcgc aaactataat gtatcaaagg
     9601 taaaaacttt aattaccctt tctcaacaaa gagttctctt caccatctcg gctctttttg
     9661 cctaatcgtt tggataaaag cactgaagct ctattttctt tcactttcgc aaaaaaagaa
     9721 tcttccttct tggctaataa ctctttataa gtcttgcttt gaaatgcttt tttaacaaaa
     9781 gctctatgag gttgctcttc atcaaaaaag ttcagaacaa atattctttt agctgtcaaa
     9841 tgcacaggta ttttaacatg gtttgcaaaa ctccattgct tcccatattc gtcaacaata
     9901 gaaactgacc cactgtagtg ataaatgggc ttttctggtc tcaacaatga gtgccctaat
     9961 aattcagcca tcatctgatt tttattcttg cttccacagc aataaagatg aaccgctgat
    10021 atccaatacg atataggtaa aatatctctt tcaaagcgtt ctgccacttc ttttatatca
    10081 atgctttctg caactaatgg attggaatga ttaaatagtt ttgttaaatt ctcatcatct
    10141 gaaccatggg cacaaatata tatcgtgatt ttttcatcca attgatcata attaatgtca
    10201 tcattgaaat aaattatttc aatgcgctca ggggaaaatc gctcatcgtt ttttttccaa
    10261 agttcaacca ttgatttcaa atcaccagcc tgatgccttg agaaa
//
LOCUS       DBNCWK010000112        10302 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.112, whole genome shotgun sequence.
ACCESSION   DBNCWK010000112 DBNCWK010000000
VERSION     DBNCWK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10302
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.112"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..841)
                     /locus_tag="AC06IO_002286"
     CDS             complement(<1..841)
                     /locus_tag="AC06IO_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIO9875460.1"
                     /translation="MKILVTGASGFIASQFVTDLLISGHEIICCVRNTKHTQRIFPGA
                     QVIFCDFINDTKPEIWSKRLQGIDVVINCVGILYHPDERIIWNVHYETPKALFDACIN
                     SGVKKIIQISALGIDKVDVNYAASKKAIDDYLLTLSIPSVIVRPSYVYGKGSYGGSSL
                     FRGIAGTPFFTAIPGQGTQKFQPISLNDLSQAIVRLVSTPVTETIILHAVSKKIITLE
                     EIIIKLRSWLGFTKTRIIHIPLTFIRLLTRLGDLLPYSTINTVSYKMLASDNITTQEE
                     TQRF"
     gene            1085..1873
                     /gene="truA"
                     /locus_tag="AC06IO_002287"
     CDS             1085..1873
                     /gene="truA"
                     /locus_tag="AC06IO_002287"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIO9875461.1"
                     /translation="MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISV
                     VCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANTFLPKDICVKWGKEMPENFHARY
                     SAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
                     QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDV
                     LNAKDRKLGAETAPSYGLYLVQVTYPKEFGLLQNNPGPLFLWEK"
     gene            1874..2497
                     /locus_tag="AC06IO_002288"
     CDS             1874..2497
                     /locus_tag="AC06IO_002288"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIO9875462.1"
                     /translation="MNPSRIRVKMCGMTRSEDIQYAIDLGVDAIGLIFYPQSARNVSL
                     EKARIIVNNIPPFVDIVAVLVNPEQSFVQLIINEIPVQLLQFHGEESSEFCRQFNKPF
                     IKAIHPKTAIQIQSAVDEFFDASAILLDTPSDKGRGGTGLTFDWNIIPENLSKPYILA
                     GGLNESNILEAITMCHPYAVDVCSGIEASPGVKDHLKMSRFIKAIWG"
     gene            2499..3698
                     /gene="trpB"
                     /locus_tag="AC06IO_002289"
     CDS             2499..3698
                     /gene="trpB"
                     /locus_tag="AC06IO_002289"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIO9875463.1"
                     /translation="MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQD
                     FLSELHTELKDYVGRPNPLYHAVHLSKKIGGAQIYLKREDLNHTGAHKINNTIGQALL
                     AKRMGKTRVIAETGAGQHGVAAATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAE
                     VVPVTSGSKTLKDALNEALRDWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEAR
                     AQHMEKTGRLPDALVACVGGGSNAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASL
                     IAGKPGVLHGNRTYLLCDEYGQVKDTHSVSAGLDYPGVGPEHAYLKDTGRVIYKAIND
                     SEALDAFRLLTHTEGIIPALESSHAVAYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVA
                     AIDGITI"
     gene            3724..4542
                     /gene="trpA"
                     /locus_tag="AC06IO_002290"
     CDS             3724..4542
                     /gene="trpA"
                     /locus_tag="AC06IO_002290"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIO9875464.1"
                     /translation="MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSG
                     ADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDDVLNMVRQFRKTDTETPVILMGY
                     LNPIEQYGYDLFAQQAVEAGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
                     ERMNFINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPE
                     MAAQVAEFADGVIVGAALINEIIEAYEAKKDPLQASGALLSSMRQAIDNIGSMV"
     gene            4539..6194
                     /locus_tag="AC06IO_002291"
     CDS             4539..6194
                     /locus_tag="AC06IO_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIO9875465.1"
                     /translation="MTEQTEKRAHFIRQLIIDDLANGKHQGIVTRFPPEPNGYLHVGH
                     AKSICLNFGLAEEFKGKCFLRFDDTNPIKEEEEYVNAIIDDVRWLGFEWCAMTHSSDY
                     YHELYELAIYLIKKDMAYVDSLSMEEIRAFRGTLQEPGRESPYRNRTIEENLDLFARM
                     KAGDFPDGTHVLRAKIDMKSGNLNMRDPVLYRIRHARHQRTGDEWCIYPMYDYAHPIS
                     DALEKITHSLCTLEFQDHRPLYDWLVDNLPLPAKPVQTEFARLNLSHTVTSKRKLREL
                     VEKKIVTGWDDPRLPTLRGMRKRGYPPAAIRQFCEIIGISRSDSVIDMSLLEECVRAE
                     FNKTAKRALCVMDPLKVVIVNYPENKVEQLQAAFYPQNPESESRNLPFSREIYIEKSD
                     FMENPPNKYFRLSPGAEVRLRHAYVIKCQEVIRNDQGEITELHCTYDENTLGKNPEDR
                     KVKGVIHWVSSSHAYPVTIYQYDRLFTDPNPAREEDYFQFLNHNSLQTLQGFCEPAMA
                     EQAEGDVFQFERLGYYCVNSVAEGCVQAFHRVVDLKDTWGKVS"
     gene            6204..7574
                     /gene="cysS"
                     /locus_tag="AC06IO_002292"
     CDS             6204..7574
                     /gene="cysS"
                     /locus_tag="AC06IO_002292"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIO9875466.1"
                     /translation="MLHLYNSLTRKKEPFVSLRPGKIGMYVCGITVYDHCHLGHARSM
                     VAFDVMVRYLRSQGFDVTYVRNITDIDDKIIARASERDVSIDELTAQYIDAMNNDTHA
                     LNILPPDHEPRATGHIETIIRLIQRLLEKGNAYVSENGDVCYEVDTFPEYGKLSHKDI
                     EGLVSGSRVEIVKEKRSPLDFVLWKKAKPGEPSWPSPWGEGRPGWHIECSAMAMHELG
                     EQFDIHGGGLDLQFPHHENEIAQSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYT
                     IADVLKEHHPEVIRYFLLSSHYRSPLNYSEENLLNAKKALIRLYQAVKDVPPQTADSK
                     LDEYWQEQFNQAMNDDFNTPVALSVLFQLAHEVNKSNSPALAHTLKNLAGILGFLQKD
                     PESFLQSGLAEEEKLVIEQLIAERLKARAERNWTKADQIRADLLSKGIELEDGATGTT
                     WRRIAE"
     gene            7746..9659
                     /locus_tag="AC06IO_002293"
     CDS             7746..9659
                     /locus_tag="AC06IO_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HIO9875467.1"
                     /translation="MNIKTYLKKNDLTLRFVGWFFLINSFIFWLVGLGYLKSILLNAS
                     LFKNYIADYSSLSGKILILVFSVLNYFTYMMFLAFLPAIPIIIMAFFLPYKRIIWIIS
                     VVAATTSLIFLFVDSQVFSMFKFHLNKTILSFVFNSDWSVIFTFSRYELILSFFMIVF
                     ILFLESVVAWLVWKKIVLKERLKIGKTISLFWLGGALISYFILLLSIAQNNNLFAQQI
                     PNLPLYNQLIAYTIPDKNADDILRRYSEHHFTQPLFPNYPLHYPLHPMQCKMPEKPYN
                     IILIMVDSLRYDSLKAKYMPNTAQFAKRSWQFSQHMSAGNATQPGLFSLFYSIPSNYS
                     TAVLEQKKSPVLVDLLLQHGYQTKIIWSGSMAPLPLDRTIYKKIANLNTNGAPIDDTG
                     DKDRYSTREAIQFLTDPKTNNPFFLHIFYNAPHDYCRYQSFPQLYKPAIEECVRIGMT
                     NHVDPVPYYNRYLNAVTFIDQEISKVLGVIEKKGYLKNSIIIITSDHGQEFNDNRQNY
                     WGHTSNFTPIQLHVPLIIHWPGELARRFNYLTSSYDIVPTILQRLFACKNPVSDYSIG
                     YNLLIEGNRAPFLLVGSYINMGIFEVDRATALETSGRITITGMKAEPLDAAVPRMDVI
                     TQALDLMRKYYGK"
     gene            9789..10103
                     /locus_tag="AC06IO_002294"
     CDS             9789..10103
                     /locus_tag="AC06IO_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6482 family protein"
                     /protein_id="HIO9875468.1"
                     /translation="MANQTRENLINKAKSGSESIALIYSPIELHEYIVEIISKDSDHF
                     IHKGKEICHFNSIDEAVSRATELGAKEFFLCADNTYDECGSANSVQHFDYIPIYSKFK
                     RT"
BASE COUNT         3181 a         1799 c         2103 g         3219 t
ORIGIN      
        1 caaatcgctg tgtttcttcc tgagtagtaa tgttatcact ggcaagcatt ttatacgaaa
       61 cagtatttat agttgaatag gggagcaaat caccaagtcg ggtcaatagt cgaataaatg
      121 tcaatggaat atgtattatt ctggttttgg tgaacccaag ccaggaacgc agttttatta
      181 ttatttcttc tagcgtgatt atttttttgc ttacggcatg caaaataata gtttcggtta
      241 caggcgtact caccaatcgt actattgcct gagataggtc atttagagag ataggttgaa
      301 atttttgtgt cccttgccca ggtatagcgg taaaaaaagg tgttccagca atccctctga
      361 atagagaaga tcctccataa gagccttttc catacacata agagggtctt actataactg
      421 acgggataga gagtgtcagt aagtaatcat ctatcgcttt tttactcgca gcataattca
      481 catcaacttt atcaattccc aaagcagaga tttgaataat tttttttacc ccagagttta
      541 tacacgcatc aaaaagtgct tttggagttt cataatggac attccatatg attctctcat
      601 cagggtggta taaaatccct acacaattaa taaccacatc aatcccctgc aatctttttg
      661 accatatttc aggttttgta tcattgataa aatcacaaaa gataacttgt gccccaggaa
      721 aaatcctttg cgtatgtttg gtattccgta cacaacaaat gatttcatgt ccagaaatca
      781 gcagatcggt aacaaactgt gaagcgataa acccagatgc gcctgttacc agaattttca
      841 taaggatctc ttatttttga ttacaattat aatagaaata ctataacgaa tttaactcct
      901 actattcaaa tttcctcacc aggattaatt ttaatcaaca ccagtttggc atatttaggt
      961 taaatgaaat aatatgagtt catgcaaaat gataaaaccc atacaggcaa agagttaata
     1021 ctctaaaaat tatagacaaa acatagaggg tgttataaat tgaccctttt atcaggaaaa
     1081 ttgaatgcgt attgccttag tggttgaata cgatggcagc caatatcacg gttggcaggc
     1141 tcaaacgggt ttgcatacta ttcaacaagc tgttgaattt gcattatcaa aagttgcaga
     1201 cagttctatt tctgttgtct gcgcaggtag aacagatact ggggtccatg cgaccaatca
     1261 ggtgattcat tttgattgtg agaaagatcg cagcatacga gcctggatac atggtgctaa
     1321 taccttttta cctaaagaca tctgcgtcaa atggggcaag gaaatgccag aaaattttca
     1381 tgctcgttat tctgctgtga gtcgacggta tcgctatgtc atttataatg gagctatacg
     1441 acctgggtta ttacgtggta atgtgacatg gcaatatcgg cagttggatc atcgtttgat
     1501 gcaacagggt ggacaatgtc ttctggggga aaatgatttt acttcattcc gctccgtcga
     1561 atgtcaatct aatacaccaa tgcgtaatat tcatcaattg caagttacaa gacatggtga
     1621 tttggttgtt cttgatatta cagcgaatgc ctttttacat catatggtaa gaaatatagc
     1681 tggtgtattg attgctgttg gctcgggaaa acaccctgtt ggttgggtta aagatgtttt
     1741 aaatgcaaag gatagaaaat taggcgctga aactgcaccg tcatatggcc tatatttggt
     1801 ccaggtgact tatcccaaag aatttggatt gttgcaaaat aatcctggcc ctttgtttct
     1861 ttgggagaaa taattgaatc catctcgtat aagagtcaaa atgtgcggaa tgacacgttc
     1921 agaagatatt caatacgcca ttgatttagg ggtggacgcc attggcttaa tattttatcc
     1981 ccaaagtgcg cgtaatgtct cactggaaaa agccagaata atagtcaata atatacctcc
     2041 atttgttgat attgttgcag ttttggtaaa cccagaacaa tcttttgttc aactgattat
     2101 taatgaaatt cctgtgcagt tattgcaatt tcatggtgag gaatcttcag agttttgcag
     2161 acaatttaat aagccgttta tcaaagcgat tcatcctaag acagcaatcc aaatccaaag
     2221 tgctgttgat gagttttttg atgcgagcgc tatattgttg gatacgccat cagacaaggg
     2281 acgaggagga acgggattaa cttttgactg gaatataatt cctgaaaatt tatccaagcc
     2341 ttacatattg gctggtggtt tgaatgaatc taacatttta gaggcaatta ccatgtgtca
     2401 tccttatgca gtggatgtgt gcagtggtat agaagcttca cccggagtaa aagatcattt
     2461 gaaaatgagc cgatttataa aggcaatatg gggatagtat gattaaaaaa gagcttccag
     2521 atgagtttgg ccattttggc ccatatggcg gtatgtttgt agccgataca ttagttcatg
     2581 ctttaaaaca attagagcat gcctacacca aatatcgtaa cgatcaggat tttctgtctg
     2641 agttacacac agaattaaaa gattatgtgg ggcggcccaa tcctctatac catgccgtac
     2701 acttaagtaa aaagataggc ggtgcacaaa tttatctcaa gcgtgaagat ttaaatcata
     2761 ccggcgctca taaaatcaat aataccattg gacaagcctt actcgccaaa cgcatgggga
     2821 aaactcgagt gatcgcggaa acaggcgcag gtcaacatgg cgttgcagct gctacagttg
     2881 ccgctaaatt tggatttcaa tgcgttgtgt acatggggtc agaagacatt aagcgtcaat
     2941 ccagtaatgt ttatagaatg aaattgttag gtgctgaagt ggtgcctgtc acttcaggat
     3001 caaagacgtt gaaagatgct ttaaatgaag cattgagaga ttgggtgagt catgtcgacg
     3061 acacgtttta tattattggt actgttgcag gtccccatcc ttatcctcaa atggtaagag
     3121 atttccaagc tattattggt gttgaagctc gagctcagca tatggaaaaa acaggccgtt
     3181 tgccagatgc tttggtagct tgtgtcggag gtggatccaa tgcaataggc ttgttttatc
     3241 cctttttgaa tgatcagtct gtcatgattt atggggttga agcaggaggg aaaggcatag
     3301 aaaccggtga gcattcggca tctctgattg ctggaaaacc tggtgtgtta cacggtaaca
     3361 ggacctattt attgtgtgat gagtatggac aggtgaaaga cactcattca gtatcagccg
     3421 gtctggatta tcctggagtt gggcctgaac atgcttattt aaaagatact ggacgggtta
     3481 tttataaagc aataaatgat tccgaggccc tggatgcgtt tcgcctgtta actcatacag
     3541 aaggaattat tccagcatta gaatctagcc acgcggtagc ctatgccata caattggcta
     3601 aaaccatgtc aaaagaacaa agcattatag tcaatctatc tggtcgtggt gataaagata
     3661 tgcatacagt agctgctatt gacggtataa caatttaatt ttgataaatt attcaggtac
     3721 aacatgaacc gtattgataa gactctggaa aaattaaaag ccaatagaaa aaaaatgtta
     3781 agcccttata ttactgcagg cgatccatat cctgagttaa cagtaagttt aatgcatcaa
     3841 ttggttaaat caggggcaga tgtattggag ctgggaatac ccttttctga ccctatggca
     3901 gaaggtcctg ttatccaaag agcaatggaa cgagctttgg cgcattcaat tcattgtgat
     3961 gacgtattga atatggttag gcaatttcgc aaaacggata ccgaaacgcc tgtgattctt
     4021 atgggatatt taaatcctat agaacaatat ggctatgatc tttttgctca acaggcagtg
     4081 gaggctgggg ttgatggaac aatattagtt gatttgcctc ctgaggaagc tgatggagta
     4141 tcgcgagttt ggcaaaaaca tggtttgtac agtatttatt tatgttcgcc aaccacctca
     4201 gctgaaagaa tgaactttat aaatcagcat gccaatggtt atctgtatta tgtttctttg
     4261 aagggagtga caggttctga cgcgcttaag ttgccagaat taaaagctca atatctacaa
     4321 cgaaaggcac aatcaaaatt accgcttatg gttgggtttg gaataaaaac tccggaaatg
     4381 gctgcacaag ttgctgaatt tgctgatggg gtcattgttg gtgcggcact gataaatgaa
     4441 atcattgaag cctatgaggc caagaaagat ccgctgcaag caagcggggc tctattgagt
     4501 tccatgaggc aagctatcga taatattgga agtatggtat gacagaacaa actgaaaaaa
     4561 gagctcactt tattagacaa ctcattatag atgatttggc aaatggaaaa catcagggaa
     4621 tcgttactcg tttccctcct gaaccaaatg gatatctcca tgttggacat gccaaatcta
     4681 tttgtcttaa ttttggtttg gcagaagagt ttaagggaaa atgcttttta cgttttgatg
     4741 acaccaatcc aattaaagaa gaagaagaat atgtaaacgc gatcattgat gatgttcgtt
     4801 ggttgggatt tgaatggtgt gccatgacgc attcttcgga ctactatcat gagctgtatg
     4861 aattagctat ttatcttatt aagaaagata tggcttatgt cgatagcttg agtatggaag
     4921 aaattcgtgc ttttcgcggt actctacaag aaccagggcg cgaaagccct tatagaaaca
     4981 gaacaatcga agagaatctg gatttgttcg ctcggatgaa agctggtgat tttcctgatg
     5041 gaactcatgt tttgagagca aaaatagata tgaaatcagg taatttaaat atgcgtgatc
     5101 cagttttata tcgtatacgt cacgcccggc accagcgtac aggagatgaa tggtgtattt
     5161 atcccatgta tgattatgct catcctattt ctgatgcctt ggaaaaaata acacattctt
     5221 tatgtacgtt ggaattccag gatcatagac ctttgtatga ttggctggtt gataatttac
     5281 ctttgcctgc caaaccagta caaaccgaat ttgccagatt aaatttatct catactgtta
     5341 cgagcaagag aaaattacgt gaattggttg aaaaaaaaat cgttacagga tgggatgacc
     5401 cacgattgcc cacattacgc ggtatgcgca aaaggggata cccacctgct gcaattcggc
     5461 aattctgtga aataattggc atatcaagaa gtgattcagt gattgatatg agtttacttg
     5521 aggaatgtgt acgcgctgaa tttaataaaa ctgcaaaacg ggctctctgt gttatggatc
     5581 ccttaaaagt tgttattgta aattatccgg aaaataaagt agaacagctt caagcagctt
     5641 tctaccctca aaatcctgag tcagagagta ggaatttacc tttttcccgt gaaatttata
     5701 ttgaaaagtc tgattttatg gaaaatccgc ctaataaata tttccggtta tctcctgggg
     5761 ctgaagtacg cttacgtcat gcctatgtta taaagtgcca ggaagttatc cgtaatgacc
     5821 aaggcgaaat aacagaattg cattgcactt atgatgaaaa tactctcggc aaaaatccgg
     5881 aggatcgcaa ggtgaagggt gtcattcatt gggtgtcttc atcacatgcc tatcctgtca
     5941 ctatttatca atacgacagg ttatttacag atcccaatcc tgcacgggaa gaggattatt
     6001 ttcagtttct gaaccataac tctttacaaa ccctccaggg attttgtgag ccggcaatgg
     6061 ccgagcaggc agaaggagat gtgtttcaat ttgagcgttt aggatattat tgcgtcaatt
     6121 cagtggctga gggctgtgtt caagcctttc atcgggtagt ggatttgaag gacacttggg
     6181 gtaaagtgag ttgagaggca gacatgttac atttatataa ttctttgacc agaaaaaaag
     6241 aaccatttgt ttccttaagg cccggaaaga taggtatgta tgtttgcgga attactgttt
     6301 atgaccactg ccacttaggg catgcccgtt caatggttgc ctttgatgta atggttcgtt
     6361 atctgcgctc acaaggattc gatgtaactt atgtacgcaa tataactgat atagatgata
     6421 aaatcattgc aagagcctcg gagagagatg tatctattga tgaattgacc gcacaatata
     6481 ttgatgcaat gaataatgat actcatgcat taaatatttt gccacctgac catgagccaa
     6541 gggcaaccgg gcacattgaa acaatcattc gcttgattca acgtttgctt gaaaaaggta
     6601 acgcgtatgt cagtgagaat ggcgatgtgt gctatgaagt agataccttt cccgaatatg
     6661 ggaaattatc gcataaggat attgaaggct tggtttcggg ttctcgcgta gagattgtaa
     6721 aagagaaacg ttcaccgctt gattttgtgc tatggaaaaa agcaaaaccg ggtgaaccca
     6781 gttggccttc accgtggggg gaagggcggc ctggatggca cattgagtgt tcagctatgg
     6841 ctatgcatga attaggtgaa caatttgata ttcatggagg tggattggat ttgcaattcc
     6901 cacatcatga aaatgaaatt gctcaaagtg aagcggctac gggtaaacct tttgccaatt
     6961 attggctgca tgtcggaatg ttgcaggtta atggcgaaaa aatggcaaaa tcaataggga
     7021 acttttacac tattgctgat gttttaaaag agcatcatcc ggaagtcatc cgctattttc
     7081 tattaagtag tcattatcgc agtcctttga attattcaga agaaaatttg ttaaatgcca
     7141 aaaaagccct tatcaggctt tatcaagcag ttaaagatgt tccgccacaa actgcagata
     7201 gcaaactgga tgagtattgg caagagcaat ttaatcaggc aatgaatgat gatttcaata
     7261 ctcctgttgc attatcggtt cttttccaat tggctcatga ggtaaacaaa agtaattcac
     7321 ctgcattggc tcataccctg aaaaaccttg ctggtattct tggattttta caaaaagatc
     7381 ccgaatcatt cctgcaatcc ggcttggcag aggaagaaaa attggttata gaacaactca
     7441 ttgcagaacg tcttaaggca cgagcagaac gcaattggac aaaggcagat cagattagag
     7501 ctgatttatt gagcaaaggc atagagctgg aagatggtgc aacaggaaca acatggcgga
     7561 ggattgctga ataattgttc cttctatgcg cttctcttga agaagaatgg acttctatct
     7621 catacttaat atagactgca attacctttt cagaaatttt aaaacaggta tattggctac
     7681 attaattgct atttattgac tggattgttc attaagataa attcgaaatt acatttgaat
     7741 tcgtaatgaa tataaaaaca tatctaaaaa aaaatgattt aaccttacga tttgttggat
     7801 ggttcttttt aataaactca ttcatatttt ggcttgttgg cttggggtat ttaaagtcca
     7861 ttttattaaa tgcctccctg tttaaaaatt atatcgccga ttattcaagt ctatcaggaa
     7921 aaattctcat tctggttttc tcagttttaa attattttac ttatatgatg tttttagctt
     7981 ttctccctgc aataccaatc ataataatgg catttttttt accttataaa cgaataattt
     8041 ggattatcag tgttgttgca gctaccacaa gtcttatttt tttatttgtt gatagtcaag
     8101 ttttttcaat gtttaagttt catcttaata aaacaatatt atcgtttgtt tttaattcag
     8161 attggagtgt cattttcact ttttccagat atgaactgat tttgtctttt tttatgattg
     8221 tctttatact ctttctggag tctgttgttg cctggttggt atggaaaaaa atagttttaa
     8281 aagaacgttt gaaaattggt aaaactattt cattattttg gctcggcggg gcattaataa
     8341 gttattttat attgctctta tcgatagctc aaaataataa tttatttgct cagcaaatac
     8401 ctaatctgcc cctttataat caactcattg cttatactat ccctgataaa aacgcggatg
     8461 atatattacg ccgctacagt gagcaccatt ttacacagcc tcttttcccc aattatccat
     8521 tgcattatcc cttacatccc atgcaatgta agatgcctga aaaaccttat aacattatcc
     8581 ttataatggt agattcactt cgctatgatt ccctaaaggc aaaatatatg ccgaatacgg
     8641 cacaatttgc aaaaagaagt tggcaatttt ctcagcatat gagtgctgga aatgccacac
     8701 aaccaggatt gttttcttta ttttattcga ttcccagtaa ttactcgacg gcagtcctgg
     8761 agcaaaaaaa atcaccagtt ttagttgatt tattattaca acatggttat cagacaaaaa
     8821 ttatttggtc tggctctatg gctccactac cattagatcg gactatttat aaaaaaattg
     8881 ccaatctcaa taccaatgga gcgccaatag atgataccgg agacaaggac agatattcca
     8941 ccagagaagc tatccaattt ttaacagatc ctaaaacaaa taacccattc tttcttcata
     9001 ttttttacaa tgcgccacat gactattgta gatatcaaag ttttccgcag ttatacaaac
     9061 ctgcaataga agaatgtgta cgaattggaa tgacaaatca tgttgatcca gtgccttact
     9121 acaatcgtta tttgaatgca gtcacattta ttgaccagga aatttcgaaa gtattagggg
     9181 ttatcgaaaa aaaaggttat ttaaaaaaca gcatcattat aattacctct gatcatggcc
     9241 aagaatttaa tgataatcgt caaaattatt ggggacatac cagtaatttt acccctattc
     9301 aactacacgt tcccttgatc attcattggc caggggagtt agctcgtcgt tttaattatc
     9361 tcaccagcag ctatgatatt gtccctacaa tattacagcg tttatttgct tgcaaaaatc
     9421 ccgtttctga ctacagcatc gggtataact tgctaattga gggcaatcga gccccatttc
     9481 tattggtagg aagttatatt aatatgggaa tttttgaagt agatcgcgct acagcattag
     9541 aaacctccgg aagaattaca attactggta tgaaggcaga gcctcttgac gctgcggtac
     9601 caagaatgga tgttattacc caggcattgg atttgatgcg caagtattat gggaaataaa
     9661 cgattgtctt taaaaataaa ttagcaatta acctttttat tctcaattta ggtcatacag
     9721 cggatgtctt taaatttcca ggcaaaaata tggctataat tatatataat ttgcttggga
     9781 attaagtaat ggcaaaccaa acacgtgaaa atttaattaa taaggcaaag agcgggtctg
     9841 aatctatcgc gttaatttat tctcccatag aacttcatga atatattgtt gaaattattt
     9901 ctaaagattc agatcacttt atccataaag gaaaggaaat atgtcatttt aattcgattg
     9961 atgaagcggt gtctcgtgct accgaattag gggcaaaaga gttttttctt tgtgcagata
    10021 atacatatga tgaatgtggt tctgcaaatt ctgtgcaaca ctttgattat atacctattt
    10081 attctaaatt taagcgtact tgagtttggc gcatcaatct tggttgcgaa ctttttgcat
    10141 ttctttttcg gttcaaattc tataatccga tgaatgacac aggctttgcc tactttaaat
    10201 cttgtagcca ctatcttcga tggttagttt tttttgctct tatttttaat acttgtctca
    10261 aaaatcctct gtacatgaca aaaaaatcta aggaatttat ca
//
LOCUS       DBNCWK010000113         9985 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.113, whole genome shotgun sequence.
ACCESSION   DBNCWK010000113 DBNCWK010000000
VERSION     DBNCWK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.113"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(274..2139)
                     /gene="vipD"
                     /locus_tag="AC06IO_002295"
     CDS             complement(274..2139)
                     /gene="vipD"
                     /locus_tag="AC06IO_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector VipD"
                     /protein_id="HIO9875469.1"
                     /translation="MKLAEIMTKSRKLKRNLLEISKTEAGQYSVSAPEHKGLVLSGGG
                     AKGISYLGMIQALQERGKIKNLTHVSGASAGAMTASILAVGMDIKDIKKLIEGLDITK
                     LLDNSGVGFRARGDRFRNVLDVIYMMQMKKHLESVQQPIPPEQQMNYGLLKQKIALYE
                     DKLSRAGIVIKNVDDIINLTKSVEDLEKFDKALNSIPTALKGAKGEQLENPRITLGDL
                     GRLRELLPEENKHLIKNLSVVVTNQTKHELERYSEDTTPQQSIAQVVQWSGAHPVLFV
                     PGRNAKGEYIADGGILDNMPEIEGLDREEVLCVKAEAGTAFEVRVNKAKQSAMEAISW
                     FKARMDSLVEATIGGKWLHASSSVLNREKVYYNIDNMIYINTGEVTTTNTSPTPEQRA
                     RAVKNGYDHTMQLLDSHKQTFDHPLIAILYIGHDKLKDALINKESEKEIFEASAHAQA
                     ILHLQEQIVKEINDGDYSSVQNYLDQIEGIITVDAKMDDIQAEKAFALCIKQVNFLSE
                     GKLETYLNKVEAEAKAAAEPSWATKILNLLWAPIEWVVSLFKGPAQDFKVEVQPEPVK
                     VSTSENQETVSNQKDINPAVEYRKIIAEGRLEHTDPSPSLQEKESIGLSTTFGGH"
     gene            2391..3131
                     /gene="lubX"
                     /locus_tag="AC06IO_002296"
                     /gene_synonym="legU2"
     CDS             2391..3131
                     /gene="lubX"
                     /locus_tag="AC06IO_002296"
                     /gene_synonym="legU2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector E3 ubiquitin ligase
                     LubX/LegU2"
                     /protein_id="HIO9875470.1"
                     /translation="MGYRIEMATRNPFDIDHKSKYLREAALEANLFHPETTPTMLTCP
                     IDSGFLKDPVITPEGFVYNKSSILKWLETKKEDPQSRKPLTAKDLQPFPELLIIVNRF
                     VETQTNYEKLKNRLVQNARVAARQKESTEIPDIFLCPISKTLIKTPVITAQGKVYDQE
                     ALSNFLIATGNKDETGKKLSIDDVVVFDELYQQIKVYNFYRKREMQKNQIQPSVSSGF
                     GFFSLNFLTSWLWGAEEKKEKTSSDMTY"
     gene            3252..9929
                     /gene="sidH"
                     /locus_tag="AC06IO_002297"
     CDS             3252..9929
                     /gene="sidH"
                     /locus_tag="AC06IO_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidH"
                     /protein_id="HIO9875471.1"
                     /translation="MKRTIETYIIYLKEDLKIADTCKTIKDGLLKSITDKTHFSEELA
                     AYFERDNPNAPFKVNTTDPTQVAVLKKLLNALENAEKSFRAIENIDISRDRYTAMIAK
                     DAVMVSYKAVHEIYAALQLINHSNSDIQDIVGPHIQKLLPQMALASKALGNFAPEHPE
                     ESAGAVLAGVVNMLPTEKPTESESLGKLSNLIFELPHYFEELQKLIATGASGIATKSI
                     TSAEDYQSAMIKKANETKYYFEQLSSKSGLSAIPSYLSIVKRLIAHSTDLVNAGAPLT
                     KQAYLDAVEKLEDIKHNILPQLISELEMVEESMGLKPGLLTDPALEQMNKYYTQLAEQ
                     VDNIAKAAGVLDTVSDYSDSIGGKIVRFLAGDSKKLDVGPKLTPAPDLGVLMDDVFIQ
                     KRRSNQESRLNEARLGSEDKSVLAAANRFFDKIGSYNSIHKAWSKWSLANISQSEKDA
                     LIKEYKQFQPHFAALYPDIDKLVVDALTQPTGSNIVSRLYSSDYKQLWSSDHFKQVLS
                     CKDSVLSSIQQSLAQSEFKAKLIEKTMSHSEETAYSMNNKTTNLTTRVQPFEPLKFTL
                     EDDKPVEYYHKKVIAASNQILELERAQKGVAEFFNYIQKKYPHENPSFDSLDESDKEF
                     LRKAYKTFQLQLLALKHDDINTRLVSSLTSSKPTDPPLRLTDLVSLKSGIDDYLNEKI
                     NDLNQDKTTLLDKEEEAREEQYAKDPLVARGAELEKQTLFGQMSKLKLSKSVDDFFNK
                     KFQTYLKDNLSPEVWKQLSSNGETLDFDKIPYLEFHKDSPEVAMYKQLINSIHYMKNG
                     LENLESLNDYGDPNNIYHRTRFVMTTFNALVMNICFSKYYVMEAGNNPGLKAIVQEGL
                     DLLKPLEGMPLIGDYLKTTEKQEPPKQNIITAWKKQQAVVESGLSKGPKPKTDQQLIS
                     EQLAKIQEAIDNFDGDLEVSDSAREKIKTQIGEFAKGISGLSFGPGSAKKILAALTKL
                     ETQLSNLDKESPEVTLGKLKDIHSELNAQFRAAAEFTEYHSGQKFGSYSNNISTIVNN
                     FCNGLVSNLPLKQEPAPKIKAPEKPVTPVITGATNPHEAVFGTKHEEFNSVYQPYLLL
                     KRITDEFRDQNNPYKPSFDELKEEAVSYYDKIQPLLESVDSKFDKNFIAKHKESSTLL
                     KAIDEVMSMRQRINNPESSFAKLKDLHLEGDFEKEENKEKFRQLYKEIQPYLHKIDST
                     YDQTQFLEGLKTAKDFSGALQRIMNEENALQQSTSLKDTSYLQLIAESLYQIPVKLNK
                     LKVETDTPEPSKEEIDANVKAFVEGLNGLSFGPGSVKKILSTAAKLQMQLSDIGKEGR
                     ELTMGRLKEIQAEFGTILMAAADNAEFHLGLKPGTYSRTVSERFEKFYSSLIVNLPLE
                     KDQTGLELLIDTTSTQKRLAREMERLERVKEDTSAIDTKKSIFGTEHEQFSTLYQPYA
                     SLRHIAKDIEDGVHMNLYERTLEELKEEASNEYKKIQPYLAKINPEFTEDYISKTDGE
                     YSLLHAIDRVFEERHKINKPSSPFDKLRDLYLDGNFEKEENKEQFLQLYAELQPHLIK
                     INYQYDLAYFLRELQTPEDFKAATERIINDESKLQELITGLDDTKRLKVKLCEERISY
                     FIDLLKKQELEVGPEKIQAFKEKIFFNYIHANVNSALEAKIGSHAEQFLQFIEKDFLD
                     KKNEILDKITIDQDMEEEIAKAIDRIAPDIINNKIESFKKLLFDSYIQSDIKNSLHNE
                     LGIYTGLFIDKISPEIHLHQSEILGNVAFDSKMGAEIGSKINAITPGILLNNNPLKEA
                     YVDLNNTLKEINTLLDEENKKARDNPCRNEKIAKLTSLKDRLSDLDSIPKENTVEFLK
                     KMQEETRSSLKSLESNDALINIYDVLNSLKETIENGSDLPEIKKDKLQMISDVQNILS
                     NSDKDTAERLNLAVQILNDSNPEVLSKTKGNFLIGEAFKGQVFTNYINTKISEQLNNE
                     LGPYGAVFLKQIMPDFIAKKSEIIKEIAIDNMETGLETQFKIHAPAIFEKNKELKAAY
                     EQLNAHLKEVQSLIEAEEKKPKGNPCREEKIAALRSHQSQLMNTQRIPDHETLRFLQE
                     QNKSAKSFMGKLEKYDTMIGVYDSLTEIREHVSNHKSLSKEIKDEKIQEISKMEDMLK
                     TTSKEPSIRLAEVKAHGLSDQCKNVLLKNSDNFLVSFFKTLFSKLFNIKNENETLVSS
                     FKQRLQNIKGPEPVATPMETPENEAPLVNANITRF"
BASE COUNT         3382 a         1827 c         1892 g         2884 t
ORIGIN      
        1 catatcgctt ccataaataa gcgatcatct tcaccatgac gacctttagg ctgtggaagt
       61 aatttttcta aacgagacca catctcgtca tcaatgacca tccgtgacat tccctctccc
      121 caaccctctc ccgcgaaagg caccatccta ctatcaaagc tcctagcgcg ggagaggggg
      181 aagatcctgg tgaagttgaa aatacgagct caattcatga ttaaatcttg tctaaaaaaa
      241 cttcagggac gttgcctaat atgtagaaca gagttaatgg ccgccaaatg tggttgaaag
      301 accaatactt tctttttctt gcagggaggg ggatggatcg gtatgctcga gccttccttc
      361 cgcgatgatt tttctatact ctacggcagg attgatatcc ttttgattag agactgtttc
      421 ctgattttca gaggtagaaa ccttaaccgg ttcaggttga acttcaactt taaagtcttg
      481 ggcagggcct ttaaacaggg aaacaaccca ttcaattggt gcccaaagaa gattcaatat
      541 ctttgtggcc cagctaggct cagcggctgc tttagcttct gcctcaactt tattcaagta
      601 agtttccagt tttccttcac ttaaaaaatt aacttgttta atgcataagg caaaggcttt
      661 ctctgcctgg atatcatcca ttttggcatc gactgtgata atgccttcaa tttgatctaa
      721 ataattttgc acgctactgt aatcgccatc attgatttct ttgacgattt gttcctgaag
      781 atgcaaaata gcttgcgcat gcgctgaggc ctcaaaaatc tccttctctg attctttatt
      841 aattaaagca tcttttaatt tgtcatgtcc gatataaagt attgctatta atggatggtc
      901 aaatgtttgc ttgtgagaat ccagtaattg cattgtgtga tcatagccat tttttactgc
      961 gcgcgctctt tgttctgggg ttggagaagt gtttgtggtt gtgacttcgc ctgtattaat
     1021 ataaatcata ttgtcaatgt tataatagac tttttcgcga tttaacacgg acgaactggc
     1081 atgaagccat tttccaccta ttgtagcctc aaccaaagaa tccatgcgtg ccttaaacca
     1141 gcttatcgct tccatagcac tttgtttagc tttattaaca cggacttcaa atgcggtgcc
     1201 tgcttctgct ttgacacaaa gcacctcttc tctgtcaagc ccttcaattt ccggcatatt
     1261 gtccagaata cctccatcag cgatgtattc tcctttggca tttctgccag gtacgaaaag
     1321 tactggatgg gccccagacc actggacaac ttgtgctata gattgttgag gggttgtatc
     1381 ttcactgtag cgttccaact catgttttgt ttgattagtt actactacag acaaattttt
     1441 aataagatgt ttattttcct caggcagcaa ttctcttaag cggcccaagt caccgagagt
     1501 tatgcgagga ttttcaagct gctctccttt ggcacctttc aacgcagtag gaatactatt
     1561 aagtgcttta tcaaattttt ccagatcttc tactgatttt gtcaagttga taatgtcgtc
     1621 tacattcttt atcacaattc ctgctcttga taacttgtct tcatagagag ctattttttg
     1681 tttaagcagg ccataattca tttgttgttc tggaggaatg ggttgctgaa cgctttctaa
     1741 atgctttttc atttgcatca tgtaaataac atccagaaca ttacgaaagc gatcccctcg
     1801 cgctcgaaaa ccaacgccac tattatctaa tagcttggtg atatccaatc cttcaatcag
     1861 ttttttgatg tctttaatat ccatgccaac ggctaatata ctcgcagtca ttgctccagc
     1921 agatgccccg ctgacatggg tcagattttt tatttttccg cgctcttgta acgcttgaat
     1981 catacccaaa taggatatgc cttttgcccc gccgccactg agcaccaaac ctttgtgctc
     2041 aggcgcacta acagaatatt gtcctgcctc agtctttgat atttccagta aattcctttt
     2101 taatttacgg ctttttgtca taatttcagc aagtttcaaa cagaatttaa tttcttatag
     2161 ttttattata gcaattaatt ttgactttgt gatttttaaa taaacaattg tgtgagccat
     2221 tgattggggg atcctcctcg aaccaatata agcatgtagt cttttttacc ggcagcttga
     2281 gatacggtgc tctttattag cgaaagcaca aataatgaag gagtgatacg cttataaaac
     2341 aaataaattg cataaaaata aatctaatgg taaaatattg catcatttaa ttggggtata
     2401 gaatagaaat ggcgacacga aatccttttg atattgatca taaaagtaaa tacttgagag
     2461 aagcagcatt agaggccaat ttatttcatc cagaaacaac accaacaatg ctgacttgcc
     2521 ctattgacag cggatttcta aaagatcccg tgatcacacc agaaggtttt gtttataata
     2581 aatcatctat cttaaaatgg ttagaaacga aaaaagaaga cccacaaagc cgtaaaccct
     2641 taacggctaa agatttgcaa ccattccccg agttattgat tatagtcaat agatttgttg
     2701 agacacaaac gaactatgaa aaattaaaaa acagattagt gcaaaatgct cgggttgctg
     2761 cacgccaaaa agaatccact gaaatcccgg atatatttct ttgcccaata agtaaaacgc
     2821 ttattaaaac acctgtcatt accgctcaag ggaaagtata tgatcaagaa gcattaagta
     2881 actttcttat cgcaacgggt aataaagatg aaacaggcaa aaaattatcc attgatgatg
     2941 tagtggtgtt tgatgaactc tatcaacaga taaaagttta taatttttac cgcaaacgcg
     3001 aaatgcaaaa aaatcaaatt caaccttcag taagtagtgg ttttggcttt tttagcttga
     3061 attttctcac ctcatggtta tggggagctg aggagaaaaa agaaaagaca tcatctgata
     3121 tgacgtacta acgctccgtt tttgcgcaat attgattcaa agtgaagtaa attttgagag
     3181 aaaaaaccac gatactggca tgaatcaact atattaataa tatagattgt taatttgctg
     3241 gtcgatgtgg catgaaaaga accattgaaa cctacatcat ctatttaaaa gaagatttaa
     3301 aaatagctga tacttgtaaa acaataaaag atggtttgtt aaaatcgatt accgataaaa
     3361 cccatttttc tgaggaattg gctgcttatt ttgaacgaga taaccctaat gcccctttta
     3421 aagttaacac gactgatcct acccaagtag ctgttctgaa aaaattgctt aatgctctgg
     3481 aaaatgctga aaaatccttt cgagctattg aaaatattga tatcagtcgt gataggtaca
     3541 ctgccatgat cgctaaagat gcagtcatgg tcagttataa agcggtgcat gaaatttatg
     3601 cggcgcttca actaatcaat cacagtaatt cggacataca agatatagta ggtccacata
     3661 tccaaaaatt attaccacaa atggctttag ccagtaaggc attaggaaat tttgccccgg
     3721 agcatcctga ggagagtgcc ggggcagtgc ttgctggtgt ggtcaatatg cttccaactg
     3781 aaaagccaac cgagtcagaa agtctgggta aactcagtaa cctcattttt gaattacctc
     3841 attacttcga ggagttacaa aaattaatcg ctaccggggc atcagggatt gccaccaaat
     3901 caattaccag tgctgaagac tatcaatccg caatgataaa aaaggcaaat gaaacaaaat
     3961 attattttga acaactcagt tccaagagcg ggctatcagc aatcccaagc tatctttcaa
     4021 tagtcaaaag attaattgcc catagtactg atctggttaa tgccggtgct cctctgacaa
     4081 aacaagctta tttggatgct gtcgaaaagc ttgaagacat taaacataat attttacccc
     4141 aattgatttc agagctggaa atggttgaag aaagcatggg tttaaaacca ggtttactga
     4201 ccgatcccgc tcttgaacaa atgaataaat actatacgca gctggcagag caagttgata
     4261 atattgccaa ggcagctgga gttcttgata ctgtttcaga ttattctgat tcaatagggg
     4321 gaaaaatcgt tcgttttctt gctggagata gcaaaaaact ggatgtgggg ccaaaattaa
     4381 cgcctgcacc tgatttaggt gttttgatgg acgatgtatt tattcagaag agaagatcta
     4441 atcaggaaag ccgacttaat gaagccagac ttggctctga agacaaaagc gtacttgccg
     4501 cggccaatcg tttctttgat aaaatcggct cctacaatag cattcacaaa gcatggtcca
     4561 agtggagttt ggcaaatatt agtcagagtg aaaaggacgc tttaatcaag gaatacaaac
     4621 agtttcagcc acattttgcg gcattatatc cagatattga caaactggtt gtagatgctc
     4681 ttacccagcc taccggcagc aatatcgttt ccagactata cagttccgat tataagcaat
     4741 tatggagttc tgatcatttt aagcaggtat tatcctgcaa ggactccgtg ctatccagta
     4801 ttcagcaaag tcttgctcaa tctgagttta aagcaaagct cattgaaaaa accatgagtc
     4861 actcagaaga gacagcttat tcaatgaata ataaaacaac taatctaact actcgggttc
     4921 aaccgtttga accactaaaa tttactttgg aggacgataa accggttgag tattaccata
     4981 agaaggttat tgcagcatct aaccaaattt tagaacttga aagagcacag aaaggggtag
     5041 cggagttttt taattatatt cagaaaaagt atccccatga aaacccatcg tttgattctc
     5101 tggatgaatc ggataaagaa tttcttcgca aggcttataa aacatttcaa ctacaattat
     5161 tggcgttgaa gcatgatgac attaacactc gccttgtaag cagcctaacc agttcaaaac
     5221 caacggaccc tcctcttcgc ctcaccgatc tggtatctct taaatcgggt attgatgatt
     5281 atttaaatga gaaaattaat gatttaaatc aagataaaac tactttgctg gataaggagg
     5341 aagaagctcg cgaagaacaa tatgcaaaag atccgttggt agcgaggggg gcagaacttg
     5401 agaaacaaac actctttgga cagatgagta aattgaagtt atccaaatct gttgatgatt
     5461 ttttcaataa gaaatttcaa acttatctta aggacaatct ttctcctgaa gtatggaaac
     5521 agctctcatc aaatggagag acgcttgatt ttgataaaat accgtatctg gaatttcata
     5581 aagacagtcc tgaagtcgcc atgtacaagc aattaataaa ctccatacat tacatgaaga
     5641 atggcctgga aaatctggag tcgttaaatg actatggtga ccctaataac atttatcacc
     5701 gcacgcgatt tgtcatgacc acgtttaatg ctctggttat gaatatctgc ttcagtaagt
     5761 actatgttat ggaggcagga aataatcccg gattaaaggc aatcgtccag gaaggcttgg
     5821 atttactaaa accattagag ggtatgccac tgataggtga ttatttaaaa accacagaaa
     5881 aacaggagcc ccccaagcaa aatattatta ctgcatggaa gaaacaacaa gcggttgtag
     5941 aatctggttt atcaaaagga cctaagccta aaacagatca gcaattaatt agtgagcaat
     6001 tggctaaaat tcaggaagcc atagataatt ttgatggaga cctggaagtt tcagattcag
     6061 ccagggaaaa aataaaaact caaattggcg aatttgccaa ggggatcagt ggcttgtctt
     6121 ttggccctgg ttctgcaaaa aaaatactgg ctgccttgac caaactcgaa acccagcttt
     6181 ctaatctgga taaggaaagt cctgaagtaa cacttggtaa acttaaggac attcattccg
     6241 aattgaatgc gcaattcagg gcggctgcag aatttaccga gtatcattca ggtcaaaaat
     6301 ttggctccta ctccaataac ataagcacta ttgtcaataa tttttgtaat ggtcttgtta
     6361 gtaatctgcc tttaaaacaa gaaccggcac caaagattaa ggcaccagaa aaacctgtta
     6421 ctcctgttat tactggtgca actaaccctc atgaggcagt ttttggcaca aaacatgaag
     6481 aatttaatag cgtttatcag ccctatctct tgttaaagcg tattactgat gagtttcgag
     6541 atcaaaataa tccatataaa ccttcttttg acgaattaaa agaggaagcg gttagttatt
     6601 atgacaaaat ccaacctctt ctcgagtctg ttgattcaaa atttgataaa aatttcatag
     6661 caaaacataa agagtcatca acactactga aagctattga tgaagtgatg tcaatgcgcc
     6721 aacgtattaa taatcctgaa agcagttttg ctaaacttaa ggatttgcat ttggaaggag
     6781 actttgaaaa agaagaaaac aaggaaaaat ttcgacagct atacaaggaa attcagccgt
     6841 acctccataa aatcgatagt acttatgatc aaacgcaatt tttagaaggt ttaaagacag
     6901 caaaggattt cagtggtgct ttgcaaagaa ttatgaatga ggaaaatgct cttcaacaaa
     6961 gtacgtcact gaaagatact tcttatttac aattaattgc agaaagcctc tatcaaattc
     7021 cagtcaagct caataagtta aaagtggaaa ctgacactcc agaaccttcc aaagaggaaa
     7081 tagatgcgaa tgtgaaagca tttgttgaag gattaaatgg attatccttt ggtccaggtt
     7141 cagttaaaaa gattctgagt actgccgcta aactgcagat gcagctttct gatattggta
     7201 aagaaggacg agaattaaca atggggcgtt taaaagaaat tcaagcagag ttcggcacca
     7261 ttttaatggc ggctgctgat aatgcggaat ttcatttggg cctaaagccc ggtacgtatt
     7321 ctcgtactgt gagtgagcga tttgaaaagt tctatagttc tctgattgtc aatttacctt
     7381 tggaaaaaga tcaaacgggt ttggagttat taattgatac aacaagcaca caaaaacgtt
     7441 tggcaagaga aatggaacgc cttgaacgtg ttaaggaaga caccagtgca atcgatacga
     7501 aaaaaagtat atttggtaca gagcatgagc aattcagtac tttatatcaa ccatatgcca
     7561 gcttaaggca tattgcgaag gatatagaag atggggtgca catgaatctg tacgagcgga
     7621 cattggagga attaaaagaa gaagcttcga atgaatataa aaaaattcaa ccttatcttg
     7681 ctaaaattaa tcctgaattt actgaggatt acatttccaa aacagatggt gagtactcat
     7741 tactccatgc cattgacagg gtattcgaag agcgtcataa aattaataaa ccatccagcc
     7801 cttttgataa attaagggat ttgtatttgg atggcaattt cgaaaaagaa gagaataaag
     7861 aacaattttt gcagctttat gcggagcttc aaccccatct tatcaaaata aactaccaat
     7921 atgatctcgc ttattttctt agagagttgc aaacaccgga agattttaaa gccgcgacag
     7981 aaagaattat taatgatgaa agtaaacttc aggaattgat taccggtttg gatgatacaa
     8041 aacgattaaa ggtaaaatta tgtgaagagc gtataagtta ttttattgat ctgctaaaga
     8101 aacaggagct ggaagttggg ccagaaaaaa tacaagcctt taaagaaaaa atatttttta
     8161 attacatcca tgctaatgtc aatagtgccc ttgaagctaa aattggttct catgccgaac
     8221 agtttctaca atttattgaa aaagattttc tcgataaaaa aaatgagatt ttagataaaa
     8281 ttactattga ccaggatatg gaagaggaaa ttgcaaaagc aatagacaga atagcccctg
     8341 acattataaa taataaaata gagtctttta aaaaattact atttgacagt tatattcaga
     8401 gcgatattaa aaactctctg cacaatgaat tgggaattta taccggttta tttatcgata
     8461 aaataagccc ggaaattcat ttgcatcaat ctgaaatatt gggaaatgtt gcatttgaca
     8521 gcaaaatggg ggctgaaatt ggctcaaaaa ttaatgcgat tactcccgga attttgctga
     8581 ataacaatcc attgaaagag gcgtacgttg atttaaataa tacattgaag gaaattaata
     8641 cattacttga tgaggaaaat aaaaaggcca gggataatcc ttgtcgcaat gaaaaaatag
     8701 ccaaattgac ttctctaaag gatcgcttga gtgatttgga cagtattcct aaagaaaata
     8761 cagtggaatt ccttaagaag atgcaagaag aaacaaggtc ttcgctaaaa tctctggaaa
     8821 gcaatgacgc cttaatcaat atttatgatg tacttaattc tttaaaagaa accatagaga
     8881 acgggagtga cttacccgaa atcaaaaaag acaagttaca aatgatttcc gatgttcaga
     8941 atatcctctc gaattctgat aaagacacag ccgaacgttt gaatttggca gtgcaaatat
     9001 taaatgactc taaccccgaa gtcttgtcaa aaactaaagg aaactttctt attggagaag
     9061 catttaaagg acaagttttt actaattata ttaatactaa aatcagcgag caattgaaca
     9121 acgaactcgg tccttatgga gcggtattct tgaagcaaat catgcctgat tttattgcca
     9181 aaaaatctga aataatcaaa gaaatagcca ttgataatat ggaaacaggg cttgaaaccc
     9241 agtttaaaat tcatgctcct gctatttttg aaaagaataa ggagttaaaa gcagcttatg
     9301 agcaattaaa cgcccatcta aaagaagtac agagcctgat tgaggcagaa gaaaagaaac
     9361 caaaaggtaa tccctgccgt gaagaaaaaa tagccgcatt gagatcccat cagtctcagt
     9421 taatgaatac ccaacgtatt ccggatcatg agacactcag gtttttgcag gagcagaata
     9481 aaagcgcgaa aagttttatg ggtaaattag aaaaatatga cacgatgatc ggtgtgtatg
     9541 actcactgac agaaataaga gagcatgtca gtaatcataa gagtttgtca aaagaaatca
     9601 aagatgaaaa aattcaggaa atatcaaaga tggaagacat gctaaaaaca actagcaaag
     9661 aaccttctat tcgtttggca gaagtcaagg ctcacggtct aagtgatcaa tgtaaaaatg
     9721 tattacttaa aaattctgac aattttctgg tgagtttctt taaaacgcta ttttccaaat
     9781 tatttaatat taaaaacgaa aatgaaactt tggtttcatc attcaaacaa aggctgcaaa
     9841 atatcaaggg tccagagccc gtcgctacgc cgatggagac tccagaaaat gaagcacctt
     9901 tagtgaatgc caatattacc agattctaat tcgaattagt tttgatttaa tcagacataa
     9961 tctttcattt cagtcaaaac aacaa
//
LOCUS       DBNCWK010000114         9924 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.114, whole genome shotgun sequence.
ACCESSION   DBNCWK010000114 DBNCWK010000000
VERSION     DBNCWK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.114"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            101..475
                     /gene="sdhC"
                     /locus_tag="AC06IO_002298"
     CDS             101..475
                     /gene="sdhC"
                     /locus_tag="AC06IO_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIO9875472.1"
                     /translation="MNKKRPINLDLSSLKFPPMAIASILHRVSGVLIFLLFPFVLFIL
                     SCSLQSEESFAQTKIILANPYYKLLLWAFGASMIYHVIAGIRHLIMDMGFGESLRAGR
                     MSAVSIIALAVILAIFLGIWIW"
     gene            469..816
                     /gene="sdhD"
                     /locus_tag="AC06IO_002299"
     CDS             469..816
                     /gene="sdhD"
                     /locus_tag="AC06IO_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIO9875473.1"
                     /translation="MVNNVTSLTGNGLKDWLIQRVTAVYFAAYLIFLFGFILANPDLN
                     FSQWHLLFANKVFQIATTIGLMALTLHAWIGIWTVTTDYMKCTVIRLSVQLLVVLWLL
                     GQFIWGLMIIWGQ"
     gene            818..2587
                     /gene="sdhA"
                     /locus_tag="AC06IO_002300"
     CDS             818..2587
                     /gene="sdhA"
                     /locus_tag="AC06IO_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIO9875474.1"
                     /translation="MTVARNRFDAVIIGAGGAGMRAALQMTNSGLKVALLSKVFPTRS
                     HTVSAQGGITAALGNADEDDWRWHMYDTVKGADYIGDQDCIEYLCKTGPEAVYELEHM
                     GLPFSRMDNGKIYQRQFGGQSKHFGGEQAARTCAAADRTGHALLHTLYQQNLKAKTHV
                     FSEWYALDLVKNSQGRIAGVTAMCMETGEIVFFQTRVCILATGGAGRIFQSTTNAFIN
                     TGDGFGMALRAGIPLQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLINKDGERFMERY
                     APRVKDLASRDVVARAMALEIRAGKGFDPKGVDHVKLKLDHLGADLIMSRLPGIRELS
                     MKFAGVDPIVEPIPVVPTCHYSMGGIPTNMHGQVITKTDGKEHVVEGLYAVGECACVS
                     VHGANRLGGNSLLDLVVFGRAAGIHVEELWQSNQLPDMSYVSEDDVVASLVRYERWEN
                     SKDGESHAVIRDEMQRVMQEDFGVFRTGEVMASGLKRLQALRDRLAHAKLDDKSQIFN
                     TERVEAMELDNLMATAYATALSAIARTESRGAHSREDYPKRDDANWIKHTLYFEEGEV
                     IDYRPVNTSPKYVEPFEPKERVY"
     gene            2600..3322
                     /locus_tag="AC06IO_002301"
     CDS             2600..3322
                     /locus_tag="AC06IO_002301"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIO9875475.1"
                     /translation="MADSRNVILSIYRYNPEVDAKPYMKDYEIEIPAKSDPMLLTLLE
                     RLKAEQDPSITYRRSCREGVCGSDGMNINGTNGLACITHLSQLKTDKIVIRPLPGFPV
                     IRDLAVDMTQFYQQYERIEPYLQNDEVAPARERLQSPEERAQLDGLYECILCACCTSS
                     CPSYWWNPDKFVGPAGLLQARRFLADSRDKATQHRLDLLQDPFSVFRCRTIMNCTNVC
                     PKGLNPTQAIAKIRTQMLTQET"
     gene            3380..6190
                     /locus_tag="AC06IO_002302"
     CDS             3380..6190
                     /locus_tag="AC06IO_002302"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIO9875476.1"
                     /translation="MSSSDLQKEWASSYLSGGSMAYVDSLYEDYLADPDSVSEDWRAV
                     FSALPKVNGATKEVSHRDIRDYFLQNADKKLNKIIQIADSQQYQVASLINDFRSLGHL
                     AAKLDPLEMTERMPVPRLELAYHNLADVDVNRTFFAGTSFNGPEMTLGEIYNALRETY
                     CRSIGIEYMHISDTEVTEWLQHKMESVRGCPEFSKQEKLNILKDLIAADGLERYLGTR
                     YVGQKRFSLEGGDSLIPMMKELIRRSGVNQVKELVIGMAHRGRLNVLVNVLGKEPGLL
                     FQEFEGKIKYERTGDVKYHLGFSSDIKTESGAIVHLALAFNPSHLEIIGPVVEGSVRS
                     RLGRRNDLAKKDKVVPIVIHGDAAFAGQGVVMETFNFSQARGYCTGGTIHIVINNQIG
                     FTTSNPLDARSTLYCTDVAKMVQAPVIHVNGDDPEAVVFATQVAFDFRMKFKRDIVID
                     LVCYRRHGHNEADEPSVTQPKMYKKIKSMPTLREKYGEQLVSANLLTKTELDKLVDSY
                     REALDKGKAVVDLVHEDYEGKQSLDWTPYINAKWTDKVDTTISKEELEKISSQLNKLP
                     DGMKLHPVVERLLAERNKMTAGEIPMNWGYAEIMAYASLLHEGYGVRISGQDSGRGTF
                     AHRHAALHDIEKGDTFIPLEHISTNPKRYFTVIDSVLSEEAVLAFEYGFASSEPSFLV
                     IWEAQFGDFANGAQVVIDQFISSGEQKWGRLCGLVMLLPHGYEGQGPEHSSARLERYM
                     QLCAQHNIQVCTPTTPAQIFHLLRRQVIRNFRKPLIVMTPKSLLRHKLAVSPLEALFK
                     GKFHTVIPEIDALDAQKVTKVVLCCGKVYYDLLQMRRDKNLNHVAIVRIEQLYPFPKK
                     ALMAELEKYQKANEVVWCQEEPQNQGVWFSSQHNMRDCLRSDQTLQYAGREFAAAPAV
                     GSPGLHAEQQVALVEQALLGKK"
     gene            6226..7455
                     /gene="odhB"
                     /locus_tag="AC06IO_002303"
     CDS             6226..7455
                     /gene="odhB"
                     /locus_tag="AC06IO_002303"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIO9875477.1"
                     /translation="MSIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKV
                     VLEVPSPVDGVLSDILFNTGDTVGSGDLLAKISQSVSVESQKTEKEEKPVKKEEIKIS
                     ESESVSTKEDKSTSPVVRRMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQ
                     TAEVQKESSMRALSFGGREEKRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVM
                     DMRSQYKDSFEKKHGVKLGFMSFFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIA
                     VSTERGLVVPVIRDADQLSMAEIELAINDAASKARTGKLSMEEMQGGTFTITNGGVFG
                     SLLATPIINPPQTGILGMHKIEERPIVEKGQIVIRPMMYVALSYDHRLIDGKDSVQFL
                     VSVKELLEDPSRLLLNV"
     gene            7518..8681
                     /gene="sucC"
                     /locus_tag="AC06IO_002304"
     CDS             7518..8681
                     /gene="sucC"
                     /locus_tag="AC06IO_002304"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIO9875478.1"
                     /translation="MNLHEYQAKQLFASYGLPVPRGEVAYNVEDALLVASQLSTSRWV
                     VKAQVHAGGRGKAGGVKLVSSKDELAAVAKSMLGTRLVTYQTDARGQPVNAILVEETC
                     EIDKELYLGAVVDRSTRRVVIMASTEGGVEIEKVAHETPEKIFKVVVDPLVGVMPFQC
                     RETAFKLGLKDDQIKQFTHLMMGLGKMFVDCDLSLLEINPLVITKSGQLICLDGKINI
                     DGNALFRQPKLKNMRDVSQEDDRENRASDWELNYIPLDGTIGCMVNGAGLAMATMDVI
                     KLHGGEPANFLDVGGGATKERVSEALKIIVSDEKVKGILVNIFGGIVRCDLIADGILA
                     AVKEVDVKIPVVVRLEGNNAQLGAEILNKSNLNVIAATSLTDAAKKIVAAVSE"
     gene            8756..9631
                     /gene="sucD"
                     /locus_tag="AC06IO_002305"
     CDS             8756..9631
                     /gene="sucD"
                     /locus_tag="AC06IO_002305"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIO9875479.1"
                     /translation="MSVLVDKHTKVLCQGFTGKQGTYHTEQALAYGTKMVGGVTPGKG
                     GQMHLGLPVFNTMREAVEETQADASVIYVPAPFCKDSIIEAAEAGVKLIVCITEGVPV
                     LDMLQVKVYIENNTQARLIGPNCPGIITPGECKIGIMPGYIHLPGKVGIVSRSGTLTY
                     EAVKQTTDKGFGQSTCIGIGGDPIPGTSFIDALSLFEKDPKTEAIIMVGEIGGSAEEE
                     AAEFIKSHVKKPVVSYIAGVTAPAGKRMGHAGAIISGGKGTAAEKYAALEAAGVKTVR
                     SPADLGDAIAEVTGW"
BASE COUNT         2971 a         1672 c         2342 g         2939 t
ORIGIN      
        1 tctcaataag tctaaagggt gaatgtgaaa taattgaatg ggattcaata tttagaagaa
       61 aatatattga tgacattttc ttatgaggaa gaaattaata gtgaacaaga aaagaccgat
      121 caatcttgat ttaagcagtt tgaaattccc gcctatggct atcgcatcga tattacatag
      181 ggtttcagga gttctcatat ttttattatt tcccttcgta ttgtttattc ttagttgttc
      241 cttgcaatca gaggaatctt ttgcacagac taaaataatt ttagctaatc cctactataa
      301 acttctgtta tgggcttttg gcgcctctat gatttatcat gtcatcgcag gtataagaca
      361 tctgataatg gatatgggat ttggtgagag tcttcgtgct ggtcgtatgt cggccgtttc
      421 aattatagct cttgcagtta ttttggcaat ttttctagga atctggatat ggtaaacaat
      481 gtcaccagct taacaggaaa tgggttaaag gattggttaa ttcaaagagt tacagctgtc
      541 tattttgcag cctatttaat ttttttattt ggatttatat tggcaaatcc tgacttaaat
      601 ttttctcaat ggcatctttt gtttgccaat aaggtatttc aaattgcaac aacaattggg
      661 ttgatggctt taacattgca tgcctggata ggtatttgga cagtaactac agattatatg
      721 aaatgtactg tgatacgttt atcagtacag ttattggttg ttttatggct ccttggtcaa
      781 tttatctggg gccttatgat catttgggga caatagcatg acagtggcac gtaacagatt
      841 tgatgcggta attataggag ctggcggtgc aggaatgcga gcggcactcc agatgacaaa
      901 ttctggttta aaagtagcgt tgttatcaaa agtatttcca acccgctcac atactgtgtc
      961 ggctcaaggt ggtattacag ctgctttggg aaatgctgat gaagacgact ggcgctggca
     1021 catgtatgat accgtgaaag gtgctgatta cattggtgat caggattgta tagagtatct
     1081 atgtaaaacc gggcctgaag cagtttatga actggagcat atggggctac ctttttcacg
     1141 aatggataat ggaaaaatct accaacgtca gtttggtggc cagtcaaaac atttcggtgg
     1201 agaacaagcg gctcgtacct gtgctgcggc agatagaaca gggcatgctt tgctgcacac
     1261 tctttatcaa cagaatttaa aagctaaaac tcatgtattt agtgaatggt atgctctcga
     1321 tctagtaaaa aactcgcaag gaagaattgc aggtgttact gctatgtgta tggagactgg
     1381 cgagattgta ttttttcaga ctcgagtttg tattttggcg actggaggag ccggtagaat
     1441 ttttcaatct acaacgaatg cttttattaa tactggcgat ggctttggaa tggcactaag
     1501 agctggtata cctcttcaag acatggaaat gtggcaattt catccaactg gtatagcggg
     1561 agctggtgtt ctggttacgg agggttgtcg aggtgaaggt ggttatttaa taaacaaaga
     1621 tggtgaacgt tttatggagc gttatgctcc cagagtaaag gacttggctt cccgggatgt
     1681 ggtcgctcgc gctatggctt tggaaattcg agctgggaaa ggatttgatc caaaaggtgt
     1741 cgaccatgtt aagttaaagc ttgaccatct gggcgctgat ttgattatgt ctcgcttgcc
     1801 tggcattcgc gagttatcaa tgaagtttgc cggtgtggat ccaatcgttg agccgattcc
     1861 agttgttcca acttgccact acagtatggg aggaataccc accaacatgc atggccaggt
     1921 tataactaaa actgatggca aagagcatgt ggtggagggg ttgtatgctg taggggagtg
     1981 tgcctgcgtt tcagtgcatg gtgccaatcg attaggtggt aattccttat tggatttggt
     2041 tgtgtttggc cgtgcagccg ggatacatgt tgaagaatta tggcaatcaa atcaattgcc
     2101 tgatatgtct tatgtaagtg aagacgatgt agtggcttcc ctggttcgtt atgagcgttg
     2161 ggaaaactca aaggatggtg aaagccatgc ggtaatacga gatgaaatgc aaagagtaat
     2221 gcaggaagat tttggagtat tccgtaccgg tgaggtaatg gcttcaggat tgaaacgttt
     2281 acaagccctg cgtgatcgat tagcacatgc taaacttgat gataaaagtc aaatttttaa
     2341 tacagagcgc gttgaagcta tggaattgga taatttaatg gcaactgctt atgcaacggc
     2401 tttatctgca attgcgcgaa ctgaaagcag aggagcgcac agcagggaag attatccaaa
     2461 acgagatgat gcaaactgga ttaagcacac attgtatttt gaggaaggtg aggtgataga
     2521 ttatcgtccg gttaatacct cacctaagta tgtcgagcct tttgagccga aagaacgcgt
     2581 ttactaatga ggatatttta tggctgatag tagaaatgta atcctgtcga tttatcgcta
     2641 taatcctgag gtggatgcca agccttacat gaaagattat gagatagaaa tcccggctaa
     2701 aagtgatccc atgttactta ccttgcttga gcgcttgaaa gctgagcaag acccttctat
     2761 tacttacaga cggtcatgtc gagaaggagt atgtggttct gacggtatga atatcaatgg
     2821 gactaatgga ttagcttgca ttacacatct ttcacaatta aaaacagata aaattgtcat
     2881 tcgtcctttg ccaggttttc cagtaattcg tgacttggca gttgatatga cacaatttta
     2941 tcagcagtat gaacgcattg agccttattt acagaatgat gaagtagcac ctgctcgaga
     3001 gaggttacaa tctcctgaag agcgggcaca attggatggc ttgtatgagt gcattttatg
     3061 cgcttgctgt accagttcat gtccttctta ttggtggaat cctgataaat ttgtaggccc
     3121 tgctggactt ttacaagcta ggcgtttttt agcagacagt cgtgacaagg caacgcaaca
     3181 tcgtttggat ttattacaag atcctttcag tgtatttcgt tgtcgtacta ttatgaattg
     3241 cacgaatgta tgcccgaagg gactcaatcc aacgcaagcg attgcaaaaa ttcgcacgca
     3301 aatgttgacg caggaaacgt aataaatcag tccttgttac tcagaaagtt tctgagtgaa
     3361 ccctttggag agaatgataa tgagcagttc tgatctgcaa aaagaatggg cttcttccta
     3421 tttgtctgga ggaagtatgg cttatgttga tagtctttat gaggattacc tcgcagaccc
     3481 tgattcagtg tcagaggatt ggagagcggt atttagcgct ttacccaaag taaatggtgc
     3541 taccaaagaa gtatctcata gggacattcg cgattatttt ttgcagaatg ctgacaagaa
     3601 gcttaataaa ataattcaaa ttgcagatag tcaacagtac caggttgcca gtttgattaa
     3661 tgattttaga tcattagggc atcttgccgc aaaactggat ccattggaaa tgacggaacg
     3721 catgccagta ccgcggctgg agcttgctta tcataatctt gccgatgttg atgttaatcg
     3781 cacttttttt gctggtacca gctttaatgg gccggagatg actttggggg aaatatacaa
     3841 cgcccttcgc gaaacatact gtcgcagtat aggcattgag tatatgcata tatccgatac
     3901 tgaagtcact gaatggttac aacataagat ggaatcagtt cgtggctgtc ctgaatttag
     3961 taagcaggaa aaattgaata tccttaaaga tttgatagca gcagacgggt tggagcgtta
     4021 tctaggaacc cgatatgttg gtcaaaaacg attttcgctt gaggggggag attctttaat
     4081 tccaatgatg aaagaactca ttagaagatc tggtgtaaat caggttaaag agcttgtcat
     4141 tggaatggca catagagggc gcttaaatgt gttagtgaat gttctcggta aagaaccagg
     4201 actattattt caagaatttg aaggaaaaat taaatacgaa cgaactggcg atgtgaaata
     4261 tcatctgggt ttttcctctg acataaaaac agaatcaggt gccattgtgc acttggcttt
     4321 ggcgtttaac ccttcacact tggaaattat tgggccagta gttgagggat ccgtacgttc
     4381 acgtttggga agacgcaacg atttggctaa aaaagataaa gtagttccca ttgtcattca
     4441 tggtgatgct gcttttgcag gtcagggcgt ggttatggaa acctttaact tctcccaggc
     4501 acgtggttat tgtactggtg gaacgatcca tattgttatc aacaatcaga taggatttac
     4561 aacgagcaat cctttggatg cccgttcaac tctctattgt acagacgttg ccaaaatggt
     4621 tcaggcacca gttattcatg tcaatggaga tgatcctgag gcagtagttt tcgctactca
     4681 agtcgctttt gatttccgaa tgaagtttaa acgagacata gtaattgacc ttgtctgtta
     4741 cagacgtcat ggccacaatg aagctgatga gccttcagtg acccagccaa agatgtataa
     4801 aaaaatcaaa tccatgccta cattacgtga aaaatatgga gaacaattgg tcagtgctaa
     4861 tttattaact aaaacagaat tagacaaatt ggtcgattcc tacagagaag cactagataa
     4921 aggcaaggcg gttgtcgatt tggttcatga agattatgaa ggaaagcaat ctttagattg
     4981 gactccttat ataaatgcca agtggacgga taaagtagac acaaccatta gcaaggaaga
     5041 attggaaaaa atttcatccc aattaaacaa attgcctgat ggaatgaaac tgcatcccgt
     5101 tgtggagcgt ttgttagctg aacgaaataa aatgaccgca ggtgaaatcc ctatgaattg
     5161 gggctatgct gagatcatgg cttatgccag tttattgcat gaaggctatg gcgtaagaat
     5221 ttctggacaa gatagtggcc gaggaacttt tgcccataga catgctgcac tccatgatat
     5281 tgaaaaaggc gatactttta ttcctttgga acatatatca accaatccca agcgctattt
     5341 tactgtgata gattcagttc tttccgaaga agcggtatta gctttcgaat atggctttgc
     5401 ttcgtctgag ccatctttcc tggttatttg ggaagctcaa tttggtgatt ttgcaaatgg
     5461 agcacaagtt gttatcgatc aatttataag ttccggtgag caaaagtggg ggcggctgtg
     5521 cggattggtg atgctattac cccatggcta tgaaggacaa ggccctgagc attcttctgc
     5581 ccgattggag cgttatatgc agctttgcgc acagcacaat attcaagtat gcactccgac
     5641 aactccagca caaatatttc atttgttaag aagacaagtt attcgtaatt tcaggaagcc
     5701 tttaattgtc atgactccca aaagtctttt acgacataaa ttggctgttt cacccttgga
     5761 agctttgttt aaaggaaagt ttcatacagt aattccggaa atcgatgcct tggacgcaca
     5821 aaaagtaaca aaggtggtgt tatgctgtgg taaggtttat tatgatttat tgcaaatgcg
     5881 ccgcgataaa aacttaaacc acgtagcgat tgtccgtata gagcaattgt atccatttcc
     5941 caaaaaagca ttaatggctg aattggagaa atatcaaaag gctaatgaag ttgtctggtg
     6001 tcaggaagag ccacaaaacc aaggagtctg gttctcttct cagcataata tgcgtgattg
     6061 cttgcgttct gaccaaacct tgcaatatgc tggcagagag tttgcagctg cacctgcagt
     6121 tggtagtccc gggttgcatg cggagcaaca agttgctttg gttgagcaag cattacttgg
     6181 taaaaaataa atctaaacaa tataatatta agagaaggta taaccatgtc tattgaagtc
     6241 aaagtacctg ttctacccga gtcagtagct gatgcaacag tggctgcgtg gcataagaaa
     6301 gtgggtgata aagtatctcg cgatgagaac ctagttgatc tggaaacaga taaagttgtg
     6361 cttgaagttc catcacctgt ggatggggta ttgtctgaca ttctgtttaa taccggagat
     6421 acggttggtt caggagactt actggcaaaa atttctcaaa gtgtttctgt agaatctcaa
     6481 aaaactgaga aagaagagaa acctgtcaaa aaagaagaaa taaaaatttc tgaaagtgaa
     6541 tcagttagca caaaagaaga taagtcaacc agtccggtag taagacggat gatggctgaa
     6601 aatgatttga aagccgggca gattcaaggt accggtaaag atggcaggat caccaaagaa
     6661 gatgttttat cttacataga atccaataga gaaaaatcaa accaaactgc tgaagttcaa
     6721 aaagaatctt ctatgagagc actttctttt ggtggcaggg aagaaaaacg tgtccctatg
     6781 acgagactaa gagccaagat tgcggaacgt ttattacaag cacagcacaa tgctgccatg
     6841 ttaaccactt ttaacgaagt gaatttaaag gcagttatgg atatgcgttc tcagtataaa
     6901 gatagctttg aaaaaaaaca tggtgtaaaa cttggattta tgtctttttt tacgaaggcg
     6961 gtagtggagt cacttaaacg atttccggct gtaaatgctt caattgatgg ccaagatatt
     7021 gtttatcatg gcttctatga tataggaatt gctgtttcta ctgaaagagg tttagtcgtt
     7081 ccggtgatta gagacgctga tcagttaagt atggcagaaa ttgaattagc cattaatgac
     7141 gctgcatcaa aagccagaac cggaaaatta tctatggaag agatgcaagg ggggacattt
     7201 accataacta acggtggagt cttcggttca ctattagcta ctcccattat caacccaccg
     7261 caaactggta ttttagggat gcataaaatt gaggaaagac ctattgtaga gaaagggcaa
     7321 atagtaatcc gtcctatgat gtatgtcgct ttatcctatg atcatcgttt aattgatgga
     7381 aaagattctg tacaattttt agtgagtgtt aaagagctat tggaagatcc atctcgactt
     7441 ttactgaatg tttaatgagt catgtttaac tgcaagaccc gtcttgacgg gttttgcttt
     7501 tttaaaaggt aattagaatg aatttacatg aatatcaagc caagcaattg ttcgctagct
     7561 atggtttacc agttcctcgt ggtgaggtcg cttataatgt tgaggatgcc ttgctcgttg
     7621 cttctcaatt atcaacctct cgatgggttg tgaaagcaca agtccatgct ggtggaagag
     7681 gtaaagcagg tggtgtgaaa ctggtttcta gcaaagatga attggctgca gttgcgaaat
     7741 ccatgttagg cacacgtctg gttacttatc aaactgatgc cagaggccaa ccggttaatg
     7801 ctattttggt tgaagaaacc tgcgaaatag acaaggaatt atatcttggt gctgttgttg
     7861 atagatccac cagacgagtt gttattatgg cctctacaga aggtggtgtt gaaattgaaa
     7921 aggttgctca tgaaacacct gaaaaaattt tcaaggtggt tgtcgacccc ttagttggtg
     7981 ttatgccctt tcaatgtcgt gaaacagctt ttaaacttgg attaaaagac gatcaaatca
     8041 aacaattcac tcatttgatg atgggattag ggaaaatgtt tgttgattgt gatttaagtt
     8101 tattagagat aaatccgctt gtcataacaa aatcagggca actaatttgt ctggatggaa
     8161 aaattaatat cgatggaaat gctttattcc gacagcctaa actgaaaaat atgcgagacg
     8221 tttcccagga agatgatcga gaaaatcgtg ctagtgattg ggagcttaat tatattcctt
     8281 tggatgggac aataggttgc atggtaaatg gtgctggttt ggctatggct accatggacg
     8341 tgataaaatt acatggtggt gagccggcta atttcctgga tgttggggga ggcgccacaa
     8401 aagaaagagt cagtgaagct ttgaagatta ttgtgtcaga tgaaaaagtc aaaggaattc
     8461 tagttaatat ttttggtgga atcgttcgtt gtgaccttat tgctgatgga attcttgctg
     8521 ctgtaaaaga agtggatgta aaaattcctg ttgttgtcag actggaaggt aataatgccc
     8581 agttaggtgc tgagatattg aataaaagca atttaaatgt gattgctgca acgagtttga
     8641 cagacgcagc caagaaaatt gtagcagcag tttccgaata aagaataaat taattttggc
     8701 attattggta aggtagtttt caaagcggat atgccattaa attacgaggt taacaatgag
     8761 tgtattagtt gataaacata caaaagtact atgtcagggt tttactggaa aacaaggtac
     8821 ttatcataca gagcaagccc tggcttatgg gactaagatg gtgggggggg ttactccagg
     8881 taagggtggt caaatgcatc taggattgcc tgtatttaat actatgcgtg aagcagttga
     8941 ggaaacccag gcggatgcaa gcgttatcta tgttcctgca ccattttgca aagattccat
     9001 tattgaagca gcagaagcag gtgttaaact gattgtttgt attacagaag gtgtgcctgt
     9061 attggatatg ttgcaagtaa aagtgtatat agaaaacaat acacaggctc gattaattgg
     9121 tccaaattgt ccaggaataa ttacaccagg cgaatgcaag ataggtatta tgcctggcta
     9181 tatccattta cccggaaaag ttggaatcgt ttcacgttca ggaactttaa cctacgaggc
     9241 agtaaaacaa actactgata aagggtttgg tcaaagtact tgcataggaa taggtggcga
     9301 tccaattcca ggaaccagct ttattgatgc tttgtcttta tttgaaaaag atcctaaaac
     9361 agaagctatc atcatggtag gagaaattgg tggttcagca gaggaagagg cagctgaatt
     9421 cattaagtct catgtaaaaa aacctgtagt atcttatatc gctggagtca cagcacctgc
     9481 tggaaagcgt atggggcatg ccggagcaat tatctctggt gggaagggaa ccgcagcaga
     9541 aaaatatgct gctctggaag cggctggtgt gaaaacagtt cgatcccctg ctgacctggg
     9601 agacgcaata gctgaggtga ctggttggta agaaaatact ttttcttact taacagctct
     9661 tgcattatag aaataggaac tgattaagat aaattggtga tttttcctcg aatcatgttg
     9721 gtgctgtgta tcaagcgcag cccaacatac atcatgataa tattgtcact tggttaaata
     9781 agattagatc catttcataa cctgccctaa aacaaaatca ggtcttagga cccccaatct
     9841 ctttgttaaa aattttttta atttagttta ttgctatatt ccctcattaa aagcactttt
     9901 gccttggcca tggggtttct cata
//
LOCUS       DBNCWK010000115         9911 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.115, whole genome shotgun sequence.
ACCESSION   DBNCWK010000115 DBNCWK010000000
VERSION     DBNCWK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.115"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..832
                     /locus_tag="AC06IO_002306"
     CDS             <1..832
                     /locus_tag="AC06IO_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HIO9875480.1"
                     /translation="SIQIDYILALVIEKTKSAISLNFPKKNKITELINEKYRLQTKEK
                     KTIYSECSGYVQGIDYNQIKKIAYDHHVQIFILIRAGDYVYKKSPILELYTDKSTDYE
                     LLVPSIMLACVNINHARLLNQDIEFGFEQVAEIAVRALSPGINDPYTARQSVFLLGEL
                     FLYIDQYDIEQDKLYYGNNIVGQYRGFTYKGIIDSALARLRQAATRDLTVILTIYDLV
                     IQLSSLLNNKSMIKALLSHTHSLKEIINNEKLSKYDEEAFNEREKQLTEIENSLSNSD
                     "
     gene            1161..2423
                     /locus_tag="AC06IO_002307"
     CDS             1161..2423
                     /locus_tag="AC06IO_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1106 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875481.1"
                     /translation="MRNNKMGEKIPSFFHHDNKANSPQILVDDPLIIIDSELLDLLNA
                     PIDKDEKYHLLISCIRSHSPKYLNQRVMVKSHTTIEGDEHTPPRWTSLATKREYGYPT
                     DGNQNNELTTTLANIIATNELNFSSAQMVALFRELMIKEVDFTTIDQSPYLNLIRPKS
                     GNNPLKSLIAASFGDHEIIHLLHDFMSYIENRVPRDEQLLIINNQDNFEFEAMQPAEF
                     LLRLGHEDLALRLYRMGAQPTVQAFRLACSSYGTTPHYEEAMECIQFLMNSVHLDETD
                     LLEGKNSLQKASPAQYNEMLTRVLRSLQEDSKLDTGHSSLFEIIQKDYIENYQGKMFI
                     SFRDFCQKEAKTDHPLIQKYALLRIGQENVGNGLFSDYYRTESRHLAEKKWTLLNKNQ
                     RANQLTHNLIDLIDTKLGSLSTFPSARL"
     gene            complement(2518..3021)
                     /gene="msrB"
                     /locus_tag="AC06IO_002308"
     CDS             complement(2518..3021)
                     /gene="msrB"
                     /locus_tag="AC06IO_002308"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIO9875482.1"
                     /translation="MTKLISVVFYLFIIPNVMAYTPQFNKSEQLKKLTPLQYQVTQNG
                     ATEKSFDNAYWNNKEEGIYVDIVSGEPLFSSNDKYDSGTGWPSFTKPIDTSYLVFHTD
                     RSYFFIARTEIRSKYANSHLGHVFKDGPEPTGLRYCMNSAALKFIPKNEMEKAGYGEY
                     LYLFNKK"
     gene            3221..4213
                     /locus_tag="AC06IO_002309"
     CDS             3221..4213
                     /locus_tag="AC06IO_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIO9875483.1"
                     /translation="MKQKQTKNQLALKWALEHLALNDEFKIIDQQKIIETAYSTVHRI
                     TSSESNVYYLKQTPEMLYLEPQIITYLQEQRCHNIPQIIAENKHLNCFLMPSCGDETL
                     RNLFKGNIDLEMLKAGILNYTKIQRIVENQLPQLIKLGIPDWQLEKFSSLYDQLIQQE
                     ELLLSDGLSKNEITLLNKAHEICFNLCESLSEYQIPETINHCDFHENNMILNQKTGNV
                     SIIDWGETVITHPFFSLNGCLWNITFFYPIKQSDTIYEALQRQCITSWLGLHDETDLL
                     NALKIANQLNGVFAALSYKRIYDATMDQPQTVQQEHPGSIAGCLRSFLNHFHLA"
     gene            complement(4257..5453)
                     /locus_tag="AC06IO_002310"
     CDS             complement(4257..5453)
                     /locus_tag="AC06IO_002310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIO9875484.1"
                     /translation="MRLFKGILFTFFGIIFCFNSHAALEHEKLVNYISLGEFSRETAE
                     IALKKMPPLDTLSVHYDLQLYKINYKTQAPDGNLTIASGLVAMPIHPVGQVGIVSYQH
                     GTRFERNDVPSRNNEKNYIYLAAYGNSAGYMTVMPDYLGLGDNELTLHPYVQAETLAS
                     SSIDMLFAAKELANRLHYPISDKLYLAGYSEGGFSTIVMFEMLAKEYPDLPVSAVAPG
                     SAPYGWEETMHFVMLEPGPRATAYLAYFFYSLQTYKSYWSGFDEIFAPPYNTLIPELM
                     DGYHAVAEILQALPQDPLLVFQPKFSNGIISKTDRNTEILKINFNHYDFKPTAPLLLV
                     GTRGDRDVPYSGAEMAYHSFRKYSDFVWIKSVSDTLDHVQAHPFVLKEQVDFFKQFER
                     QEVMHK"
     gene            5785..6636
                     /locus_tag="AC06IO_002311"
     CDS             5785..6636
                     /locus_tag="AC06IO_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875485.1"
                     /translation="MSREEEQAKKKLEEDKVQEEKLIRSTVDDYIREFSNMLHACEQR
                     ARRKPADRAVLSAEKDAHTLREFLDDLQKAQQEGHGDLPILRTPAEWAAIDQMRQRCV
                     QYQIDPMQNHKMKNEKTEKRTHDSQVCEENLRRLQILDRYKETPRSEEEEEKERKRLI
                     NLATSGNNTFIAYEDLEKIAKSGKPFFIPPNSKEDACLSCGNGRLYKGSLSEIRMQLE
                     FDLKKDPDNEQIRTGLRRIENEIFMKPNLASPKKEKDFTEAGITPQENTYTTPNPFSI
                     KPKKEPI"
     gene            complement(6918..7886)
                     /gene="lem4"
                     /locus_tag="AC06IO_002312"
     CDS             complement(6918..7886)
                     /gene="lem4"
                     /locus_tag="AC06IO_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem4/SmdA"
                     /protein_id="HIO9875486.1"
                     /translation="MTTSVSFDETSKRNKGRVKNIVYLDFDGTITGAHGKEVISSPLC
                     EALSTKATFDERMRYKNEYDASDNKIKITENAKKFLQDVNKLHPQVKIVIISRNHENY
                     IKALLEFENIDHRNIIIYPRGVGNTIGPGEDKYKAVVSHEEKPECLPGFRLICDDDEV
                     DGEEMCNGLIHTGRSQLVKFHNEKPGQFKWGEYFKEILTNCDIAVKEYLNGKIGSRFH
                     LFTAKVDEKTGDQSFKDRYSQVKGDILKRAIINNLKNDIEKIDNLDDLKDFIKKFKQS
                     SEYMTLSKAQGLFTKVTGIKTDSQRAVEEIFSKALKDLQSPKLAMR"
     gene            complement(8386..8988)
                     /locus_tag="AC06IO_002313"
     CDS             complement(8386..8988)
                     /locus_tag="AC06IO_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HIO9875487.1"
                     /translation="MKKSMERSSDTLLEIASHQELKGEVTYQRILQLLGERAFGIVLL
                     FFALPSALPFSFIPGISLIFSVPILLFAFQMVFARKTMWLPKIIAERTIHQETIAKFI
                     HNTVPYLIKVEYFLKPRWLFMTSRFMEIIHGIVIFFLALLLMLPIPFSNFIFATLLII
                     FSLGLIEKDGLFLIIGYLGVVIYANFVYLFVLTAVKHLFG"
     gene            complement(9111..9569)
                     /locus_tag="AC06IO_002314"
     CDS             complement(9111..9569)
                     /locus_tag="AC06IO_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875488.1"
                     /translation="MEHNWYDNNSKLSVMGIICLIIFLVLFFFSLFIMPFLIWELHYN
                     VPDFVSNMIAYLEDTYYYSSPVSKTLVWLTFFIPCLITGYFSYYISCSIDKKNLLKDA
                     NIDEEQSKEISSVTNEQIKESASLGFKILILMAAIILIIFILQEFVKFTS"
     gene            complement(9716..>9911)
                     /locus_tag="AC06IO_002315"
     CDS             complement(9716..>9911)
                     /locus_tag="AC06IO_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875489.1"
                     /translation="GGLKKVIALILSVGPILISASCTISSSADNVLQADGQSASDEHQ
                     KHKGHGGYGGGRGGGGVGGR"
BASE COUNT         3447 a         1874 c         1628 g         2962 t
ORIGIN      
        1 gtcaattcag atcgattata ttcttgcctt ggttattgaa aaaacaaagt ctgctatttc
       61 acttaatttt cccaaaaaaa ataaaataac agagcttata aatgaaaaat atcgtctaca
      121 aacgaaagaa aagaaaacaa tttactccga atgcagcggt tatgttcaag gcattgatta
      181 taatcaaata aaaaaaatcg catacgatca tcatgtgcaa atatttattc ttataagagc
      241 tggagattat gtctataaaa aaagtcccat actagaattg tatactgaca aatcaacaga
      301 ttatgaactc ttggttccat ctattatgct agcgtgcgtc aatataaatc atgccaggct
      361 cttaaatcag gacattgaat ttggattcga gcaagtcgca gaaattgctg tacgtgcttt
      421 atctccagga attaatgacc cttacacagc aagacagtct gtgtttttat tgggcgaatt
      481 attcctttat atcgatcaat atgacattga acaagacaaa ttatattatg gtaataacat
      541 agtgggtcaa tacagaggct tcacttataa gggtataatc gattccgcct tggcacgatt
      601 aagacaagcg gcaacaagag atctgacagt catattgacg atttatgatt tggttattca
      661 actaagcagc ttattaaaca ataagtctat gatcaaagcc ttattaagcc atactcactc
      721 attgaaagaa atcattaaca atgaaaaatt atcaaaatat gatgaagaag cttttaatga
      781 gagagaaaaa caactaaccg aaatagaaaa ttccctatcc aactctgatt agaataaagt
      841 tttaattcga ttgacgctgg atattagatc aactactcac aggtataggt ctgaaaaact
      901 gaaacacggt ttgtccttat attatccagg caactattgt aaaacactct atgaattttt
      961 catcaagttt taactctcca ttttttcatc agatagcatg aaaatattct tgacttatat
     1021 aaacaatcta atcaagatga aatcatcatg gctaaaatag gagtaacaag gctctattga
     1081 ttattacaaa caaaacatgg gttcgaggct tttgttgcat cagctatcgc ttttaaactt
     1141 tttgaaatag gttcttaact atgcgaaata ataaaatggg agaaaagatt ccaagttttt
     1201 ttcatcatga taataaagcc aactcacctc aaatattggt agatgatcca ttaatcatta
     1261 tagatagcga attgttggat ctgttaaacg ccccaattga caaagatgag aaatatcacc
     1321 tcctaatatc ctgcattcgc agtcacagtc ctaaatattt aaatcaaaga gttatggtta
     1381 aaagccatac aacgattgaa ggtgatgagc atactccccc ccgctggaca tctttggcaa
     1441 caaaaagaga atacggttat ccaaccgatg ggaatcaaaa taacgaattg actacaaccc
     1501 tggcaaatat cattgctact aatgaattaa acttttcttc cgctcaaatg gttgccttgt
     1561 ttagagaatt aatgataaag gaagtggatt ttaccaccat agatcaatcc ccttacctca
     1621 atttaatacg ccccaaatct ggcaataacc ctctcaaaag tttgatagca gccagttttg
     1681 gggatcatga gattatccac ttgctgcatg actttatgag ttatattgaa aatagagttc
     1741 ctcgtgatga acaattattg attatcaata atcaagataa ttttgaattt gaagcgatgc
     1801 aaccggctga atttttatta cggctaggtc atgaagattt ggctttacgc ttatacagga
     1861 tgggagctca accgactgta caagcattcc ggctggcttg ctccagttat ggcaccaccc
     1921 ctcactacga agaagcgatg gaatgcattc aattcttgat gaattctgtt catctggatg
     1981 aaacggattt actagaagga aaaaattcct tgcaaaaagc aagcccggca caatacaacg
     2041 agatgttgac aagagtatta cgctccctgc aggaagattc gaaacttgat actggacact
     2101 cctctctctt tgaaataata cagaaagact acatagaaaa ttatcagggt aaaatgttta
     2161 tttcttttcg cgatttttgt cagaaagaag caaaaactga tcatccactg attcaaaaat
     2221 atgccctttt gcgcatcggg caagaaaacg taggtaatgg cctgttctca gattattacc
     2281 gcactgaatc cagacatttg gccgagaaga aatggacttt actcaataaa aatcaaagag
     2341 ctaaccaatt aacacataat ctcattgatt taattgacac taaacttgga tcattaagca
     2401 cgttcccttc agccaggcta taatttgagg aacatacggc aagttagccc atttaagctc
     2461 taacattgat ttgagctcaa tgagttgcat aggctaactt gctgaaaaaa aggcagttta
     2521 ttttttattg aacagataaa gatactcccc gtacccagct ttttccattt cattttttgg
     2581 aataaatttc aaggcagctg aattcatgca atatcgcaaa ccggttggtt ctgggccgtc
     2641 tttaaagaca tggcctaaat gagagttagc gtatttagag cgaatttcag tacgcgcaat
     2701 aaaaaaatag cttctgtcag tatggaaaac caaataggat gtatcgattg gctttgtaaa
     2761 actgggccaa cccgttcctg aatcgtattt atcattagaa ctaaacaaag gttctcctga
     2821 tactatatca acataaatac cttcctcttt attgttccag tatgcattat caaatgattt
     2881 ttccgttgcc ccattttggg ttacctgata ttgcaatgga gtcagtttct taagctgctc
     2941 tgatttgtta aattgagggg tataggccat cacatttgga attataaaca ggtaaaatac
     3001 aacgctgatt aattttgtca tgtcataact ccacatgcat tttcatgccc acattgatga
     3061 cgcttactta tagtgtagca agcgtacccc atattagcag aaatctacgt ttccatcgga
     3121 atcaatattc atacccaaag cagaagacag gcacgatgtt tggacttaaa ttattttata
     3181 cattaaattc atattaaaga ttattagtta tgagcaaact gtgaaacaaa agcaaacaaa
     3241 aaatcagctc gccttaaaat gggctcttga gcatctggca ttaaacgatg aatttaaaat
     3301 cattgatcaa cagaaaatta ttgaaaccgc ttattcaaca gtacatagaa tcacctcttc
     3361 agaaagcaat gtttattatc tgaaacaaac accggaaatg ttgtatctgg aaccccaaat
     3421 aataacctat cttcaagaac agcgatgtca caacattccc caaatcattg cagaaaataa
     3481 acacttaaat tgctttttga tgccgtcttg tggtgatgaa actttacgga atctgtttaa
     3541 aggaaatatt gatttggaaa tgcttaaagc aggtattctt aattacacta aaattcaacg
     3601 gatagtagag aatcaactcc cacaactcat aaaattgggt atccccgatt ggcagcttga
     3661 aaaattttca tcattatatg accaactgat acaacaagaa gagcttttat taagtgatgg
     3721 actatcaaaa aatgagatta ccctcctgaa caaagcacac gaaatttgct ttaatttatg
     3781 tgaatcactc tccgaatacc aaattccaga aacaattaat cattgtgatt ttcatgaaaa
     3841 taacatgatc ctcaatcaga agacgggaaa tgtaagcatc attgactggg gagaaaccgt
     3901 tatcactcat ccattttttt ctctgaatgg ctgcttgtgg aacataacct tcttttaccc
     3961 aataaaacag tccgacacaa tatatgaagc attacaacga caatgtatta cttcttggtt
     4021 aggtctgcat gacgaaactg atttattaaa tgcgctaaaa attgccaacc aactcaatgg
     4081 ggtttttgct gcattaagtt ataagcgaat ttatgacgca accatggatc aacctcaaac
     4141 agttcaacaa gaacaccctg gctccatagc tggctgccta agatcatttt taaaccattt
     4201 tcatttagct tgactgctac atcaaaactg tgattgctga gtttcacaag gtgatattac
     4261 ttatgcatca cttcttgacg ctcaaattgt ttaaaaaaat ccacctgttc tttgagtaca
     4321 aagggatggg cctgtacatg atccaatgtg tcactgacag attttatcca tacaaaatca
     4381 ctatatttcc taaaggaatg atacgccatt tcagctcctg agtaaggtac atcacgatca
     4441 cctctagtgc caaccaataa caaaggagct gtaggtttaa aatcatagtg gttaaaatta
     4501 atttttaaaa tctcggtgtt tctatctgtc ttactaataa ttccattaga gaattttggc
     4561 tgaaaaacaa gcaacggatc ttgtggtaag gcttgaagaa tttcggcaac agcatgatat
     4621 ccatccatca attccgggat aagagtattg taaggaggag caaaaatttc gtcaaaacca
     4681 gaccaataac tcttataagt ttgcagagaa taaaagaaat aggccaaata ggctgtagcg
     4741 cgaggccctg gttccaacat cacaaaatgc atggtttctt cccagccata aggagctgaa
     4801 cctggggcaa ccgcagagac aggtaaatca ggatactctt ttgccagcat ttcaaacatt
     4861 acaatagttg agaatccgcc ttctgaataa cctgccagat ataatttatc gcttattggg
     4921 taatgcagcc tgttggctaa ctccttagca gcaaacaaca tatcaatgct actgctggca
     4981 agagtttctg cctgaacata aggatgaagt gttaattcat tatcgcccag accaagataa
     5041 tctggcatca ccgtcatata acctgcacta ttaccatagg cagcaaggta tatatagttt
     5101 ttttcattat ttctggaagg cacatcattg cgttcaaatc gggtgccatg ctgatagcta
     5161 acaattccca cttgccccac aggatgaatt ggcattgcaa ctaatcctga ggcaatagtc
     5221 aggtttccat ctggtgcctg ggttttataa ttaattttat ataattgcag atcataatgc
     5281 actgaaaggg tgtctaatgg cggcattttt tttaaagcga tttctgctgt ttccctggaa
     5341 aattcaccca aagaaatata attaaccagc ttctcatgct ccagagcagc atgggaatta
     5401 aagcaaaaaa taatcccgaa aaatgtaaaa agaatccctt tgaataaacg catcgctttc
     5461 ataaccatct ataaaaatgt ggataattac atgtttaata aggagaatca aatgaagcag
     5521 ttacttgcat taatgcaaag gacacaatct gttcctttct atttattcca atcatctaac
     5581 aaacaagtat tcaattaaaa cgcctgatga aataaatgaa gtattatcta caaatgctct
     5641 tgttgctgaa aaaatgcatg aattttatag gaaaactcag agagataagt aactccagaa
     5701 aaatatacta tcattaattt tataagataa gattattttc aattctacag tgaattgatg
     5761 ttcaaataat ttatggtaat cacaatgtcc agagaagaag agcaagctaa aaaaaaatta
     5821 gaagaagata aagtacagga agaaaaactc atcagaagta ctgtggatga ttatatccgg
     5881 gaattcagta atatgttaca tgcttgcgaa caacgagcaa gaagaaaacc tgccgacagg
     5941 gcagtactct cagctgaaaa agatgcgcat actcttcgcg aattcctcga tgaccttcag
     6001 aaagctcaac aagaaggaca tggagaccta cctatactac gaaccccggc cgaatgggct
     6061 gctatcgatc aaatgcgaca aaggtgcgta cagtaccaaa tcgatcccat gcagaaccat
     6121 aaaatgaaga atgaaaaaac agagaaacga acccatgact ctcaggtttg tgaagaaaat
     6181 ttacgccgac tacagatatt agacagatac aaagaaacac ctcgttctga agaggaagaa
     6241 gagaaagaaa gaaaaagact tataaatctt gcaaccagcg gtaataatac ttttattgct
     6301 tatgaagatt tggaaaagat agccaagagt ggtaaacctt tttttatccc acccaactca
     6361 aaagaggatg cttgtctatc ttgtggtaat gggcgtttgt ataaaggttc attatcagag
     6421 atccgtatgc aattagagtt tgacctaaaa aaggatccgg ataatgagca gataagaact
     6481 ggtttgagaa gaattgaaaa tgaaattttt atgaaaccta atttagcatc acccaaaaag
     6541 gaaaaagact ttactgaagc gggaataact ccacaagaaa acacttacac tactccaaac
     6601 ccattttcaa taaaaccgaa aaaagaacct atataagcct atgtactgat gagcttaacc
     6661 acttggagcc aaacaaccgg aaatcttgct aaagtgatga agcttgatag ttagtaacat
     6721 aagctttcta tcaaccaata aaatgccgca ccactgtcaa gacaaacaaa acaaagttat
     6781 ttaaacaatg ctggtagaac agagacatgg ctatgagaac aacttgtact gctacagtct
     6841 tcgccaaatt gaaaaattta aattgaattt gatgaggcag tcagctcata aatggcaact
     6901 gcccgatcaa atttgagcta cctcattgca agcttgggtg attgtaaatc tttgagtgcc
     6961 ttgctaaaaa tctcttctac tgcccgttgc gaatcagttt ttatgccggt aactttagta
     7021 aataaccctt gggctttgct aagggtcata tattcggagc tttgtttaaa tttctttata
     7081 aagtctttta aatcatctaa attatctatt ttttctatat cattttttaa attattaatt
     7141 attgcgcgtt ttaatatatc tccttttact tgactgtatc tatctttaaa agattggtca
     7201 cctgtttttt catctacctt tgctgtaaaa agatggaagc gtgaacctat ttttccgttc
     7261 aggtattctt ttacagcaat atcgcaatta gttaatattt ccttaaaata ttcaccccat
     7321 ttaaattggc ctggcttttc gttatggaat tttacaagtt gggagcgtcc agtatgtata
     7381 agcccattac acatctcctc cccatctacc tcgtcatcat cacaaatcaa acgaaatcct
     7441 ggtaaacatt caggtttttc ttcgtgtgat accactgctt tgtacttgtc ttcccctggc
     7501 cctattgtat ttcctacgcc gcgaggataa ataataatat ttcgatgatc aatattttcg
     7561 aattctaaaa gagctttaat ataattttca tgattacggc tgattattac aatttttacc
     7621 tgtggatgta atttattgac atcttgtaga aatttcttgg cattttctgt tattttaatc
     7681 ttattgtcag atgcatcata ctcattttta tagcgcatcc tttcatcaaa agtcgcttta
     7741 gtactcaaag cttcacacag gggggagcta atcacttctt tgccatgtgc accagttatt
     7801 gttccatcga aatccagata aacgatgttc tttaccctac ctttatttct ttttgaagtt
     7861 tcatcaaaag acaccgacgt agtcatgatt gtttcctcgc aaaaccaccc aatgcataca
     7921 tttaatcatt atgatttgtt tatgtacaat taattatttt cataagtcat attattttta
     7981 ataatatcca ttgtttttca atatcttaat gattaatcgt acgcttgcgt tcagctaaat
     8041 atgggttgca ttgaaagaaa aaagaactac cagcgaaaac ataagcttcc ccaactattt
     8101 tatggggttt actatttcga tttgctgcaa aaacaaagac catctaccca tatcatctta
     8161 ttccgtgtgt gtcatgctta atacttgatg ttagccaagc attccattat atgctgagac
     8221 tccaggatag caaggcatct taattcagtt atgtgagaac ctgaaaaatt actgcatcaa
     8281 agttattccg agtgattaaa gtggaatttt ttatttattt acatatggtt aattcaacta
     8341 taatgaaaaa agtgaactct aatggttagt aacacaagct ttcaatcaac caaacaaatg
     8401 cttcaccgcc gtcaagacaa acagatagac aaagtttgca taaatcacaa cccctagata
     8461 cccaataatg agaaataatc catctttttc tattaatccc aagctaaaaa taattaacaa
     8521 cgtagcaaaa ataaaattac taaatggaat tggtaacatc aataataggg caaggaagaa
     8581 aataacaatc ccatggatta tttccataaa gcgggaagtc ataaacaacc atctgggttt
     8641 taaaaaatat tcgactttaa ttaagtaagg aacggtatta tgtataaact tggctattgt
     8701 ttcctggtga atggttcgtt ccgcaataat ttttggtagc cacattgttt ttctggcaaa
     8761 aaccatttga aatgcaaata gtaatatcgg aacactaaaa atcagggaga taccaggaat
     8821 aaatgagaaa ggcagcgcac tgggcaatgc aaaaaataaa agcacaattc caaaggcgcg
     8881 ctctccaagc aattgcaaaa tacgttgata agtgacttcc ccctttaact cctgatgact
     8941 tgcgatttct aaaagggtat cagaagagcg ctccatgctt tttttcataa aaaatccaag
     9001 tcaaaatata taacattaat ttaccaactg tttgaggaaa atttttacct taagaatttt
     9061 taaagctcac attttctcta actctgcaga aaacacagag ttgcttcaag ttatgatgtg
     9121 aatttaacga attcttgtaa tatgaaaatg attaaaataa tagccgccat tagaataaga
     9181 attttaaatc ctaaacttgc tgactcctta atctgttcat tagttaccga agaaatttct
     9241 tttgattgtt cttcatcaat gtttgcgtct ttcagcaaat ttttcttatc aatagagcaa
     9301 gaaatgtaat aagaaaaata cccggtaatc aagcaaggta taaaaaaagt caaccagaca
     9361 agagtcttac ttacaggcga agaataataa taggtatctt caaggtatgc tatcatattg
     9421 ctaacaaagt caggaacatt ataatgtaat tcccagatta aaaaaggcat tataaacaaa
     9481 ctaaagaaaa agaggactaa aaaaataatc aaacaaatta ttcccattac agataatttg
     9541 ctgttgttat cataccaatt atgctccata ggctccttcc ataaaatgac cagctaacta
     9601 aaattattct tttttaaatt ttaagacatc agagcatcca tcgtataacc gaatatattt
     9661 taagctagat attaaaaaat agcttaacga caaataaact tacttccggc cgcgtctaac
     9721 gtcctccaac accaccaccc ccacgaccac ctccatatcc cccatgccct ttatgctttt
     9781 ggtgttcatc tgaagccgat tgcccatcgg cctgcaaaac attatccgca cttgatgata
     9841 tagtacaaga agccgaaatt aatattgggc ccaccgataa tattaaagca ataactttct
     9901 tcaatcctcc a
//
LOCUS       DBNCWK010000116         9889 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.116, whole genome shotgun sequence.
ACCESSION   DBNCWK010000116 DBNCWK010000000
VERSION     DBNCWK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.116"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            92..1381
                     /gene="hemL"
                     /locus_tag="AC06IO_002316"
     CDS             92..1381
                     /gene="hemL"
                     /locus_tag="AC06IO_002316"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIO9875490.1"
                     /translation="MSRSSDLFHKAQTIIPGGVNSPVRAFKGVGGEPVFFKSGKGAYL
                     TDVDDKQYIDYVGSWGPLILGHCHPKVIAAVDNVLHSGMSFGAPTELEIQLAEKIASL
                     MPSIEKIRMVNSGTEATMTAIRLARGFTNKSKFIKFNGCYHGHSDSLLVKAGSGLLTL
                     GIPSTPGIPQSITEHTLTADFNNLEQVAQLFEKYPNDIATVILEPVPGNMGFILPKIE
                     FLKGLRELCDQYNALLIFDEVMTGFRVGLHGAQGLFGIKPDITTLGKIIGGGMPVGAL
                     GGKREIMSFLAPEGPVYQAGTLSGNPLAMAAGLATLKEIEKINFFEDLSSTTNKLTEA
                     LSDAAENANIPLFAASLGGMFGFCFTDKNSVENYLDVASSDEVLFKKFFHAMLAQGVY
                     FAPSMYEAGFVSSMHGDLEIQKTYDAAELVLNQLKSA"
     gene            1793..3319
                     /locus_tag="AC06IO_002317"
     CDS             1793..3319
                     /locus_tag="AC06IO_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIO9875491.1"
                     /translation="MLILSKRRGLWSSMPQGTFALFFIQVVSTLSFSVLYSTLVLYMK
                     GKLGLPIATANSIMGVFIAYNFALHLLGGFWGGRLLSNRSLFCVGMIAQILGCVLLSI
                     GNTTFLYYGLAAFLTGSGLNVTCVNCMLTQQFTPEDSRRETAFLWNYAGMNIGFFIGF
                     SLSGAFQISQNYQRLFLLSSLGNLVALLICLYFWQQLHDKNTTYSRKDKPAQRKATAL
                     GIGLIFVLPFILRQLLQYADWANKLVIVTGIIMLILILILAQQQSTKEARDKIYGFAV
                     LMLVGTVFWMLYQIGPMGLTVFIDHNVQRQYGNWIIPPQWFQNINTVVIVFGGPLLGF
                     ILNRMRQNGSQINIPTQFAFALFFIGVAFAILPIGINYANQHGMVNPGWVVISFILQS
                     IGELLISPIGYAMIGALAPTSLQGVMMGMWMLNTGVGATLSSYSSNLMIIGQESTSPL
                     LTNEGYSHVFLMLGLFAIGSSVILFILVPKLRKLIKEKNLNGFKQSGLEVTERVALCE
                     "
     gene            3312..5312
                     /gene="mnmC"
                     /locus_tag="AC06IO_002318"
     CDS             3312..5312
                     /gene="mnmC"
                     /locus_tag="AC06IO_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HIO9875492.1"
                     /translation="MSSPFVPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFV
                     EGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKH
                     PLKKNDLIKCLQKWPELSVQTEKLIEHYPVLTPGYHHLAFSNNRITLTLMLGDVLECY
                     EQLLFCGDINLEHQLRESYVNAWYLDGFSPSKNQSMWSDNLFTIIAMLSKESTTVATY
                     SASSIVKTALTNAGFVIKKRKGFGPKRHMIYAHYEKAYSSSKKNRHTPWHINYPVTKE
                     ERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQQAVLFPKLSIYKSP
                     FTQFMLYSFLYANDVYKELLKHYNLGELKGSLLLAHNEREKANQQSLIHWLELYPELG
                     QLVDEKQSSELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYN
                     QKNWVVNDIDASVLILANGQQVNYFHETNHLPVKAIRGQMTTIQSTQESTKLKIPLCA
                     EGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIIWSQNVLDHWAG
                     IRAASPDYLPIVGPLPNALEFKEIYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLT
                     TIPLATEWLAGLINREISILPRKLIQAISPARFLRKKIIQGP"
     gene            5398..6183
                     /locus_tag="AC06IO_002319"
     CDS             5398..6183
                     /locus_tag="AC06IO_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIO9875493.1"
                     /translation="MQNIEFYQIDAFTDKVFHGNPAAVCILDDWLNDELLKTIAAQNN
                     LAETAFIIKDDQDFHIRWFTPNGEINLSGHATLAAGFALLESNKGPSDSIIFHHSSGQ
                     ISVEKRKEKFVLNFPRIGYKKTPSPDPIASLLDQPAIEVFETDLDYLVLLEHESQIRN
                     LQIDLLALAQLPKRGIIVTSTTTEADFYSRCFYPKHSIAEDPVTGSAHCILAPFWAEK
                     LNKNSLYAIQGLHRKGELYCEIQRNRVLISGHCRWYLRGHLVI"
     gene            6399..7517
                     /gene="prpC"
                     /locus_tag="AC06IO_002320"
     CDS             6399..7517
                     /gene="prpC"
                     /locus_tag="AC06IO_002320"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate synthase/citrate
                     synthase"
                     /protein_id="HIO9875494.1"
                     /translation="MSSKSGGLAGVVAGQSAIATVGLAGKGLNYRGYSINDLAEYASF
                     EEVAYLLHYGELPTQKELDVYIKKLVNLRHIPDVLKTVLKLIPKNTHPMDVLRTACSF
                     LGTIEPEENFKQQYEIADRLLALFPGIMCYWYTYHFKNKEISGLSEENTTGGHFLALL
                     HGRKPSKIETDMMNVSLILYAEHEFNASTFAARVTAATLADFYSAITSAIGTLRGPLH
                     GGANEAAMELIERFKTPDEAAIGIKQMLASKELIMGFGHRVYTTCDPRSDIIKKWSFR
                     LGEEKGNLLLYHISERIEEIMWQEKKLFPNLDFYSASAYHYCDIPTHLFTPIFVMSRI
                     TGWSAHVFEQRANNKLIRPTSEYIGPDSRPFPAIETRG"
     gene            7519..8967
                     /locus_tag="AC06IO_002321"
     CDS             7519..8967
                     /locus_tag="AC06IO_002321"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HIO9875495.1"
                     /translation="MHNYVEDNVKPDYDQVIADIADFVLNKEIDSVQAYETARLCLMD
                     TLGCGILALNFQECTKLMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDF
                     NDTWLAAEWGHPSDNLGAILAVADYLCRENCNHGRPPILMHDVLTAMIKAHEIQGCLA
                     LENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNA
                     GSRKSWAAGDATSRAVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFNFQRPYGS
                     YVMENVLFKLSYPAEFHAQTAVECAVKLHPIIKNRLEDIARIELITHESAIRIISKQG
                     ALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENEQFSRD
                     YHDPEKRSIANSMRIIFKDGSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFS
                     TDMVEKIIHAMSDTNTLSSMKVSDFMAMWMVN"
     gene            complement(9087..>9889)
                     /gene="ppsR"
                     /locus_tag="AC06IO_002322"
     CDS             complement(9087..>9889)
                     /gene="ppsR"
                     /locus_tag="AC06IO_002322"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HIO9875496.1"
                     /translation="FMLSDGTGITAETLGNSLITQFENIQFEKITIPYIDSTHRAESV
                     VLRINQCFSEQGTKPLVFMTLVDPEIRQAIKKAHACVFDLFSIFIGPLENELEEKSSY
                     TVGRTHGVANVKSYSHRIEAIDFALSHDDGIKTRGYDKADIILIGVSRCGKTPSCLYM
                     ALQYGILAANYPFTEEDLVGFRLPEVLRPYKQKLFGLTIDAQRLQQIRSERRPNSKYA
                     SAEQCRLEVTEVEAMYQRENIPYINSTKYSIEEISTKVLAIAGLQRKI"
BASE COUNT         3112 a         1747 c         1950 g         3080 t
ORIGIN      
        1 acattttttg tataataaat ttctgaaaca tcttgaatat taaaaataat aactgatatt
       61 tgtctaaagt ttatcatttt tgggattttc tatgagccgt tcttcagatt tatttcataa
      121 agcacaaaca atcattcccg gaggtgttaa ctcaccggtt cgagctttca aaggtgttgg
      181 aggtgaacct gtttttttca aatctggcaa aggcgcctac ctgacggatg tagatgataa
      241 acaatatatt gactatgttg gatcatgggg accacttatc ctcggacact gccatcccaa
      301 agtgatagca gctgtggata atgtattaca cagtggtatg agttttggtg ctccaacaga
      361 actggaaata caattagctg aaaaaattgc ttccctaatg ccatcaatag aaaaaattcg
      421 catggtgaac tcaggaactg aagcaactat gactgcaatt cgactggcgc gcggattcac
      481 taataaaagt aaatttatta aatttaacgg ctgttatcat gggcatagcg atagcttatt
      541 agttaaagcg ggatctggtt tgcttactct cggaattcct tcaactccag gcattccaca
      601 aagcattacc gaacatactt taacggctga ttttaataac ctggaacaag ttgctcaact
      661 ctttgaaaaa tatccaaacg acatcgctac tgtaattttg gaacctgttc ctggcaatat
      721 gggatttatc ctacctaaga tagagttttt aaaaggatta cgtgagcttt gcgatcaata
      781 taatgctttg ttaatttttg atgaagtaat gactggattt agagttggtt tacatggtgc
      841 acagggcttg tttgggatta aacctgatat cacaacactt ggaaaaatca taggtggtgg
      901 tatgcctgta ggtgctttag gtggtaaacg tgaaattatg tcctttctgg cgcctgaagg
      961 ccctgtatat caggctggta ctctttcagg aaatccattg gcaatggccg caggtttggc
     1021 tactttaaaa gagattgaaa aaattaattt ttttgaagat ttaagcagta cgactaataa
     1081 attaaccgag gcattatctg atgcggcaga aaacgcaaac attccacttt ttgctgcatc
     1141 attgggcgga atgtttggat tttgttttac agataagaat agtgttgaaa attatttgga
     1201 tgttgcttcc tcggatgaag ttttatttaa gaaatttttt catgctatgc ttgctcaagg
     1261 agtatatttt gcaccatcta tgtatgaagc tggatttgtc tccagtatgc atggtgattt
     1321 ggaaatacaa aaaacatatg atgctgctga attggtactg aatcaattaa aatctgcctg
     1381 atagcagaat tgtaccttat tacctgacgt tacttaaata caatctttaa aatcagtatt
     1441 tgggtaatta agttcattaa agcgtctaaa tgtgattgca gaacagattt ttaaatctgg
     1501 ttaactgcaa ttttaaataa ttaataaaac actagacagc tgtttttccc tatgtcatat
     1561 tccattttct agatcgcaag tatggaatct tggtgaaaaa tcagatttgc gcagaataag
     1621 taaaaagttt ctaattgaac ctaataacac aactgcagtt tgattattaa aatatattta
     1681 atttatttta gatactttac acttgctgac gaaaggtggt ttttctctga tttgtccaca
     1741 agggttagtg ttaatattca agaagagctc aactagcaag ggaaagcata caatgttgat
     1801 tttgagtaaa agaagaggac tatggagttc catgccccaa ggtacatttg cattattttt
     1861 tatacaagtg gtttctactt taagctttag tgttctttat tcaactcttg tattgtacat
     1921 gaaaggtaaa cttggtttac ccatagccac agcaaacagc atcatgggcg tatttattgc
     1981 ctacaatttt gcgctgcatc tgttaggtgg tttttggggc ggcagactgc tctccaatcg
     2041 ctccttattt tgtgttggga tgatcgcgca aattttagga tgtgttttgc tctctatagg
     2101 aaatacaact tttttatatt atggcttggc tgcattctta acaggttcag ggttaaatgt
     2161 aacatgtgta aattgtatgc tgactcagca atttacacct gaagactctc gaagagaaac
     2221 cgcatttctc tggaattatg cgggaatgaa tattggcttt tttattggat ttagcctcag
     2281 tggggcgttc cagatttctc aaaactatca aaggcttttt cttttaagca gtttaggtaa
     2341 tcttgttgct ttgcttatat gcctttattt ctggcaacaa ttacatgata aaaacactac
     2401 ttactcaaga aaggacaaac ctgcacaaag aaaagcaaca gcattgggga ttgggctaat
     2461 ttttgtactt ccatttattt taagacaatt attacagtat gctgattggg ccaataaatt
     2521 ggttattgtc actggaatta ttatgctcat attaatcttg atattagccc aacaacaatc
     2581 tactaaagaa gcaagagaca agatttatgg atttgcagta ttaatgttgg ttggtactgt
     2641 attttggatg ttatatcaaa ttggcccaat ggggttaacg gtattcattg accataatgt
     2701 gcaacgacaa tatggtaatt ggattattcc gccacagtgg tttcaaaata ttaatacagt
     2761 agtcatagta ttcggagggc cactattggg tttcatctta aatagaatgc gtcaaaatgg
     2821 atctcagata aatattccta ctcaatttgc tttcgcatta ttttttatag gggttgcttt
     2881 tgctatctta ccaataggta tcaattacgc taatcagcat ggtatggtta atccgggttg
     2941 ggtagtaata agctttatat tacaaagtat tggcgaactt ctaatatctc caattggcta
     3001 tgcgatgata ggagcgttag ccccaacctc actccagggg gtcatgatgg gaatgtggat
     3061 gctgaatact ggagttggtg ccactctatc cagttatagc tcaaacttaa tgattattgg
     3121 tcaagaaagt acttctcctt tattaaccaa tgagggttac agtcatgtct ttttgatgct
     3181 aggtttgttt gcaataggat catccgttat ccttttcatt ttagtgccta aactaagaaa
     3241 gctgatcaaa gaaaaaaact tgaatggctt taaacagtct ggcttagagg taactgaaag
     3301 agtagcttta tgtgagtagt ccctttgtcc caattataac ggcagatatc gattggcgtg
     3361 atgatctgcc gtattcctta caatttgatg atatttacta ttctgctgaa ggtggaatta
     3421 atcaaagtct ttatgttttt gtcgaaggta ataatttgat taatcgttgg caacaattac
     3481 caaccaatga atcaaatgta tttacaatcg ctgaaactgg tttcggtaca ggtatgaatt
     3541 tcttattaac ctggaaacta tgggaaaaat ttgcacctca aaatgcccgc ttacattata
     3601 tttcctgtga taaacatcca ttaaaaaaaa atgacctaat caaatgcttg caaaagtggc
     3661 cagaactgag tgtgcagact gaaaaattaa tagaacacta tcctgttttg acacctggtt
     3721 atcatcattt agcatttagt aacaatcgga ttactttaac attaatgcta ggagacgtgc
     3781 tggagtgcta tgagcaatta ttattctgtg gggacattaa ccttgaacat caattgagag
     3841 aatcctatgt taatgcttgg tacctggatg gattttcacc atcaaaaaac caatccatgt
     3901 ggtctgacaa tctgttcact atcattgcca tgctatctaa agaaagcacc acagtagcta
     3961 cctattctgc ttccagtatc gtcaaaactg ctttaaccaa tgcaggattt gttattaaaa
     4021 aaagaaaggg atttgggccc aaacgacaca tgatatacgc ccattatgaa aaagcttatt
     4081 caagttcaaa aaaaaatcgc cataccccgt ggcacataaa ctatcctgtg acaaaggaag
     4141 aaagaacagc gctcattgtt ggaggagggc ttgcaggttg ttttattgca aatagcctcg
     4201 ctaagcgtgg atgggaagtg accatattag aagaaaaaga gaaagtggga tgtggtggat
     4261 cggcaaatca acaggctgta ttgttcccta agttatccat ctataaatca ccatttactc
     4321 aatttatgct ttattcgttt ctttatgcta atgacgttta caaggaactt ttgaaacatt
     4381 ataaccttgg tgaattaaaa ggctctttgt tgttagcgca taacgagagg gaaaaagcaa
     4441 accagcaaag tcttattcat tggctagaat tataccctga attaggacaa ttagtggatg
     4501 aaaagcaatc ttctgaatta tcagggatat ccttgccatg tggtggttta tttattccat
     4561 cttccggctg gattaattcc cctgaactat gcgacattct aattgataat aaaagaattt
     4621 ctctaatcac tggcaataga gttcaatcta ttaactataa tcaaaaaaac tgggtagtta
     4681 atgacatcga cgcttcggta ctaattttgg caaatggaca gcaagtcaat tattttcacg
     4741 aaaccaatca tttgcctgta aaagccatta gaggacaaat gactacgatt caatcaacgc
     4801 aagaaagtac caaattgaag attcctttat gtgccgaagg gcatgtttta ccagctttaa
     4861 acaatagtca tagagtgggg gcaagttatg atattggcac atctgaacct gaacttaatg
     4921 ctctggatga tcaattaaac ctcgaccgac taaaacggat agcgcctgat ataatctggt
     4981 cccaaaacgt gttagaccat tgggctggta tacgagccgc atcaccagat tacttaccta
     5041 tagtgggacc tctccccaat gcgcttgaat ttaaagaaat ttattcagaa ttaaaatcta
     5101 actccaaacg atggatagct gaagcagctc cttgttatcc aaatctatat gtgtgtgcgg
     5161 cttttggatc aagaggatta acaacaatac ctctcgcaac tgaatggtta gctggtttaa
     5221 ttaataggga aatttcaata ctgcccagaa aattgataca agctatatcg cccgcacgat
     5281 tcttaagaaa aaaaattata cagggaccat aagagatata ttgaagatat attaataatt
     5341 cgacttaact tcatctttct ttatcagtta taattttaat ttttatgtga gctgattgtg
     5401 cagaatattg agttttatca aatagatgct tttacggaca aagtcttcca tggtaatccg
     5461 gctgcggttt gtattctcga tgattggtta aatgatgagc tattaaaaac cattgctgca
     5521 caaaataatt tggcagaaac tgcttttatt ataaaggatg atcaagattt tcatattcgt
     5581 tggtttactc ctaatgggga aatcaatcta tcaggacatg caactttggc agcagggttt
     5641 gctttacttg agtcaaataa aggacctagt gattctatta tttttcacca ctccagtgga
     5701 cagatttcgg tagaaaagag aaaggaaaag tttgttctga attttccaag aataggttat
     5761 aagaaaactc cttcaccaga cccaattgca tcactattgg atcaacctgc tatagaagta
     5821 tttgaaacag accttgatta tctggttttg cttgaacatg agtcacagat tcgaaatttg
     5881 caaatcgatt tgcttgcttt agcacaactc cccaaaagag gaatcattgt aacctcaaca
     5941 acaacggaag ccgatttcta ttcccgatgt ttttatccaa aacacagcat tgcagaagat
     6001 cctgttactg gctcagcaca ttgcatatta gctccttttt gggcagaaaa actcaataaa
     6061 aacagcctat atgctatcca gggattacac agaaaagggg agttatattg tgagatccag
     6121 aggaataggg tattgatttc cggtcattgt cgatggtatc tacgcgggca tttagtaatt
     6181 taataaaatc gattgagatg cattcatcat tgaagcataa tataaaaccc atttaggaac
     6241 caatggagca tgataacaat atctattcca aaaggaattc tctccaagag aataagataa
     6301 aataatcaac cgcttcaaaa ttcagtaata ataaaaacat acgcagcaat tttgactgca
     6361 ctagaaataa aaactagacc aactgatgga ggaagaaaat gagtagcaaa agtggaggtt
     6421 tagccggtgt agttgctggt caatcagcaa ttgccacagt tggactggca ggcaagggat
     6481 taaattacag aggctattca ataaatgatc tggctgaata tgcaagtttt gaggaagttg
     6541 cttatctgct tcattatggt gaacttccta cccaaaagga acttgatgta tatataaaaa
     6601 agctggttaa cctaagacat atacctgatg tgcttaaaac ggttttgaaa cttataccta
     6661 aaaatactca cccaatggat gtactgcgaa cagcatgttc ttttttaggc actatcgaac
     6721 ctgaagagaa ttttaagcaa caatatgaaa tagctgatcg tttgctagct ctttttccgg
     6781 gaataatgtg ttattggtat acatatcatt tcaaaaataa agaaatctct ggcttaagtg
     6841 aagaaaacac aacaggagga cactttctgg cgttattaca cggacgcaag cccagcaaaa
     6901 tagaaaccga tatgatgaat gtctctttga tcttgtatgc tgaacacgaa tttaacgctt
     6961 ctacctttgc tgcaagagtt acagcggcaa cactggcaga tttttactca gcaatcacaa
     7021 gtgctatagg aacattgcgt ggtcctttgc atggtggcgc caatgaagca gctatggagt
     7081 taattgagcg ctttaaaacc cctgatgaag cagcgatagg cataaagcaa atgcttgcca
     7141 gcaaagaatt gatcatggga tttggacatc gggtttatac aacttgtgat ccacgctcag
     7201 atattattaa gaaatggtca tttagattgg gtgaagaaaa ggggaaccta cttttatacc
     7261 atatttcaga acgtattgaa gaaatcatgt ggcaagagaa aaaactattt cccaacctgg
     7321 atttttatag cgcatctgct tatcattact gcgatatacc aactcatttg tttaccccaa
     7381 tttttgtgat gtcacgcatc acaggatggt ctgcgcacgt atttgagcaa agagcaaata
     7441 ataaactaat aagacctact tccgaatata taggcccaga ttccaggcca tttcctgcta
     7501 ttgaaacacg aggttaatat gcacaattat gtagaggata atgttaagcc agactatgat
     7561 caggttatag cagatatagc tgattttgta ttaaataaag aaattgatag tgtgcaggcc
     7621 tatgagactg cacggttatg cttaatggat acactcggtt gtggcatatt ggcccttaat
     7681 tttcaggaat gtaccaaatt aatgggtcct atagtaccgg gagcaactct tcctggaggc
     7741 gcgagagtac caggtactaa ttatgaattg gatccggttc aagctgcctt taacatcggt
     7801 acgatgatac gctggctaga ctttaatgat acttggctcg ctgcagaatg ggggcacccc
     7861 tctgataatt taggtgctat tttggctgtt gccgattatt tgtgccgtga aaattgtaat
     7921 catggacgcc ctcccatttt gatgcatgat gtacttacag caatgattaa agcacatgaa
     7981 attcagggtt gtttggccct ggaaaatagc ttcaatcgcg tcggccttga tcatgtcata
     8041 ttagttaaag tagcgagtgc cgctgttgct gctcacttgt ttggagcaga tcgtgatatg
     8101 atgctaagaa ctctctcgca ggtttttgtg gatggacaaa gtttgagaac atatagacat
     8161 gctcccaatg caggttcaag gaaatcatgg gcagcaggag atgctacctc ccgtgcagtt
     8221 cgtttggctt taattgccaa ggcaggggaa atgggatatc caagcgcctt gaccgcacaa
     8281 acatggggat tttatgacgt gctatttggt aaaaaacaat tcaattttca acgcccttac
     8341 ggcagctatg tgatggaaaa tgtgttattc aagttatctt atcctgctga atttcatgca
     8401 caaaccgctg tcgaatgtgc agtaaaatta catcctataa taaaaaatag attagaggac
     8461 attgctagaa ttgaattaat cacacatgag tcagcaataa gaataataag taagcaaggt
     8521 gctttatata atcctgccga cagagatcac tgcttgcaat atatggttgc agtaggactt
     8581 ctctttggtg atttaaaggc agagcattat gaagacgacg ttgccgcaga tcccagaata
     8641 gatgctttac gagctaaaat gcatgtaact gaaaatgaac agttttcacg cgattatcat
     8701 gatccggaaa agcgctctat agctaacagc atgagaatca tctttaaaga tggatcagaa
     8761 agtgaattaa taactataga ataccctatt ggtcacaaac ggagacgaga agaggggatt
     8821 cccgttttaa tgtctaaatt caagcgtaat ctgagtacaa gattctctac tgacatggtt
     8881 gaaaagatta ttcatgccat gagcgacacc aatacgctat catctatgaa agtaagtgat
     8941 tttatggcca tgtggatggt aaattaaaaa gactaacacc cacactcaat ctgttatctg
     9001 tactgatttt agcacagacc gggagggggc taagcgtata gcaaagccaa ccctccttac
     9061 taaatcgcgg ccttcaaaaa taggttttat attttccttt gtaaaccggc aatagcaaga
     9121 acctttgtcg atatttcttc tatggaatat tttgttgaat tgatataagg aatattttct
     9181 cgttggtaca tggcttctac ttctgttacc tctaatctgc actgctctgc ggaagcatat
     9241 ttgctatttg gtctacgttc agaacgaatt tgctgcaaac gttgtgcgtc aattgttagt
     9301 ccaaataatt tttgtttata aggtcttaat acctctggta agcgaaaacc caccaaatct
     9361 tcttctgtaa aaggataatt ggcagctaaa atgccatatt gcagtgccat atataagcaa
     9421 ctgggagttt tgccacaacg tgaaactcct ataagaataa tatctgcttt atcgtaacca
     9481 cgcgttttta ttccatcgtc atgtgaaagg gcaaaatcga ttgcttcaat tcgatgggaa
     9541 taggatttca catttgcaac cccatgagtc ctgcctactg tataagatga tttctcttca
     9601 agctcatttt ccaatggtcc aataaaaata ctaaacaaat caaaaacaca ggcatgcgct
     9661 tttttaatgg cttgtctaat ttctggatca actaaagtca tgaatactaa gggcttggtt
     9721 ccttgctctg aaaaacattg attaattctt agtacaacac tttctgctct gtgagttgaa
     9781 tctatgtagg gtattgttat tttttcaaat tgaatattct caaattgagt aatcagacta
     9841 ttacctaatg tctcagccgt aattcctgta ccatcggaaa gcataaaaa
//
LOCUS       DBNCWK010000117         9568 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.117, whole genome shotgun sequence.
ACCESSION   DBNCWK010000117 DBNCWK010000000
VERSION     DBNCWK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.117"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..972
                     /locus_tag="AC06IO_002323"
     CDS             <1..972
                     /locus_tag="AC06IO_002323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875497.1"
                     /translation="GFYMISNDTVNELIERDKRKNVKLSDFIITNAKPGGSRWGYIGH
                     FGNSELFLLKREHLGYETISSSTPNCITILECLCSKIIGKVLGKHYVAETIPVKIDTE
                     TIKIHQGNETESLQLDEYDDYAIASRWLSNQQMNYDLPSIDHTINDDYMLKFALMCNL
                     LGINDVKSEHYIKHTVLENGILHQHMAIIDCAGDLSAFEQEKIDYFFKKNMYIGLLRP
                     IVSAKPEQYPLVLEVFTEFLKIDVDKEFEEYEHIPSPSLEQMKVNLQKTLDLVRDSLL
                     PQLVTLIEVTTEQNSKSIPYSISFYKPGPTVEEKPLPTLTPTTINLN"
     gene            complement(1006..2391)
                     /locus_tag="AC06IO_002324"
     CDS             complement(1006..2391)
                     /locus_tag="AC06IO_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIO9875498.1"
                     /translation="MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNID
                     SKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLD
                     LMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
                     QITASYFTYLAEIERKEFLQTLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQ
                     GELEVTERNIVISRNALRYLLNQNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDM
                     QVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPI
                     IDMPVLGAVAKAKGVNKVYYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHL
                     AKAYNLNDRLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYR
                     AKPQSPISPSS"
     gene            complement(2375..3280)
                     /locus_tag="AC06IO_002325"
     CDS             complement(2375..3280)
                     /locus_tag="AC06IO_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIO9875499.1"
                     /translation="MRRWLGVQAFSYVELRSIQISTVFAFTIFVQEWLRYPRAGWTGF
                     AVMMIYAGFDNGTTIFRTYHRFLGVLLGLFTGYLLWFVGHLDYRTLILIMPLMVFFAF
                     FLVGRAYSVPTVFTVNVSVLGSGYFSTEDTLPVTYFIIDYTLCTLIAFTIILGFEYFW
                     FRHYGLMQRFIKSTQAEVIDDLYRLVRLLNQGKIKKTEWFQACIKLTDSLFEVNKLIT
                     NSQFLISSEHAVGDEFNQFVELANRIFIGLKALYMAYYTKHYHTFDYYQLYQQVQKDL
                     VHLKDFVAGEQTIDLSEGVKHATSG"
     gene            complement(3265..4197)
                     /locus_tag="AC06IO_002326"
     CDS             complement(3265..4197)
                     /locus_tag="AC06IO_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIO9875500.1"
                     /translation="MFISQLKDKLASYDRYKEHRINGLKAVFVLELMIIFYFFSSIDN
                     PYFYYFYAPITCFTVEAAGTTLKEKYLLLLYTLLGSILSIFLFGIISVYNVLFIFFVF
                     FYTLALYFLVLHHFKKMLVIVPLMLSLASYSLIYGVEADSNFYIAINNALYTLAAMAV
                     IFAGLFIFPKKYYFDIWERAFLEVITGLESLSAKICKGEVKTIAIFSGIIVMERYSKM
                     LPRNIKCYSILKITILSFELILTMSYLLSFQKQLRKEYVIVLHHYLERLCQSCKARQP
                     LILSDQELPAFKETHELEILYQLILSWNYLCADG"
     gene            complement(4172..5245)
                     /locus_tag="AC06IO_002327"
     CDS             complement(4172..5245)
                     /locus_tag="AC06IO_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIO9875501.1"
                     /translation="MKEKFATWYKIVTKHIRFFFSYSIPMFFKKARNYINLPIIVVGL
                     ALIIGLFHLFSYLLPFTDNAFVVTNVTPVAADVSGFITEIYVKNGQKVKKNDPIFSVY
                     RVPYELAYQQAMADYKEGLKKIDVFQKQIERTVALIKATDAELEKAQFALGLKKNKNV
                     AEAVSVLELKNLGYDVSTLSNKRNALQQEVAVLQAKIEQQRHTVASLKAKMDNAKVNL
                     DLTIVRAPGDGVIDNMYVSPNTPIEIHKPVFSFIDTANYYIQANFNETDLRNVRQGDK
                     AYIILRMYYFDKIFHGVVVNSLWAAERQSTAPKSQIQVVTNQNEWLLLPQRFPLQIKI
                     LDPDPDYPLNPGASAYVYISTKR"
     gene            complement(5346..6557)
                     /locus_tag="AC06IO_002328"
     CDS             complement(5346..6557)
                     /locus_tag="AC06IO_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIO9875502.1"
                     /translation="MSLEVEPKPRGNNPYANRFDNLPLKAQGRFPRVAYVLQGGGSLG
                     AYQFGVVKGLLEAGYEPDWIAATSIGAIQAAIIVGNPPEKRIEKLERFWDEIAPGNFF
                     DFLGESDITLDFYNKLSAACALLFGQPEFFFPKWCTGTIPLWGDPTTLSYYDTTPLRK
                     TLLDLIDFELLNSCPIRLSLGAVQISNGHLIYFNNINYRIEVEHIMASAALPPGFPAV
                     NIDGELYWDGGVHSNTPLEVILEAIPAENTLCFLIDCFGGRSFIPKSMSEIEERRKDI
                     SYGTHAQRTIYNYLQRQKMRNSMMELSRILTDEQKKESAHLLDIGSPHHCTLVHLAYS
                     SRIVKAASKDYNFGRVVIKKRMECGYSDAKAVLAEESQWGYLPEDGKSRLYESPNNLS
                     RLFRKRKKEVF"
     gene            complement(6994..7833)
                     /locus_tag="AC06IO_002329"
     CDS             complement(6994..7833)
                     /locus_tag="AC06IO_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIO9875503.1"
                     /translation="MKAIEVEQLIQGTLIREGGGVKLRRYIGADKTNDFEPILLFDYF
                     DSSDPLDYLAGFPPHPHRGFETITYLMEGSITHEDNRGNKGVINAGDVQWMTAGKGII
                     HSEMPSSSTGRLHGLQLWLNLPAAEKWREPRYQEKSSDQLPIEVMESGATIKVIAGRI
                     DQGLSSPIVEIATRPLFLDITLPANATIQQTIPDDYQAVLFVIEGAVNTGGQQITAGT
                     LASLTNGERIQIEGKGNENRNHCLLIAAARLHEPIVRYGPFVMNTQEEIMQAIHDFRT
                     GHF"
     gene            7979..9556
                     /gene="cegC1"
                     /locus_tag="AC06IO_002330"
     CDS             7979..9556
                     /gene="cegC1"
                     /locus_tag="AC06IO_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC1"
                     /protein_id="HIO9875504.1"
                     /translation="MNTTEHTELGNGLLMDKIEGNPYLRIDEFGVLHLRLQRFGENNL
                     PEPMELELSAGEIIAMAGDYFTQANWTMDLDLPKCELFNSPAELGKHLIRKPIDPKEE
                     NALITAYNNLAAPDVTRKEIDRIYSINNANYVPFSPTLNFYAQQLMYYFRVKDYGEML
                     VRNQTHFTPWSIRVYILGHAIALRYAHLSYELKQLATDRNYQSDNPDLSTLKISLQNK
                     NETLSSNTLLDLANRYHAQAYSIELFTFHYYSDHFATGHMSMIGDLRVVLKERFGVWG
                     NILANNLHDEVNRVGVYTVRPYDPTPNTTEAPSRARGDGKFDTCLNQFNRLACLNGMT
                     ASLKDINQVLSGSVIPEQKNFGGLVHMPDVDFNSRQHQPLLVLSKGKVYYRNNLSQIH
                     IISPSEYEALRENPEKHGYKELTSKWAAFKLVTKLRLFPYLYDGSVMPVSDEKLAEII
                     ADEKCRNPHRAPIPTPSCVPESEPTVFDWRTKASWRNNKDSLDILDGLKKHSILGAKQ
                     APAAIQEEEEVQLNLGV"
BASE COUNT         3071 a         1908 c         1723 g         2866 t
ORIGIN      
        1 ggattctaca tgattagcaa cgacacagta aacgaattaa ttgaaagaga taaaagaaaa
       61 aatgtaaagt tgagtgattt tatcattacc aatgctaaac caggggggtc tcgttgggga
      121 tatataggcc attttgggaa cagtgaatta tttttactaa aaagagagca tttaggctat
      181 gaaactatct cctcgtctac acccaattgc atcactattc ttgaatgttt atgttcaaaa
      241 ataataggta aagttttagg caaacactac gttgcggaaa ctattccagt aaaaatcgat
      301 actgaaacaa ttaaaataca tcaaggaaat gaaacagaat ccctgcaatt ggatgagtac
      361 gatgactatg ctatagcatc cagatggctg tcgaaccaac aaatgaatta tgacttaccc
      421 tcaatcgatc atacaatcaa cgatgattac atgcttaaat ttgctctgat gtgtaattta
      481 cttgggatta atgatgtaaa aagtgagcat tatattaaac atactgtttt ggaaaatggt
      541 attttgcacc aacatatggc aattattgat tgtgcaggtg atttatcagc ttttgagcaa
      601 gaaaaaattg attatttttt taaaaaaaat atgtatattg gtttattacg gcctatagtg
      661 tcggcaaagc ctgaacagta tccgcttgtt ttagaggtat ttactgagtt tctcaaaatt
      721 gatgttgata aagagtttga ggagtatgaa cacatacctt ctccatcttt agaacagatg
      781 aaagtgaatt tacaaaaaac gttagatttg gtacgtgata gtttgttgcc gcaactcgta
      841 acacttatcg aagtaacaac agagcaaaat agcaaatcaa tcccttattc tatttcattt
      901 tataaaccag gacctacagt agaagaaaaa cccttgccca ctttaactcc aaccactatt
      961 aatctaaatt agtagggtca gagtattgat aagaagttat gaaaatcaag atgaagggct
     1021 tatgggtgac tgaggtttag cacgatatcc acctgctaat tcttgatata aacgtacaat
     1081 gcttagtagc tcttgcaatt tatcctgatt taaactgatg ttgattttat cgagcgaaat
     1141 tttgctattt aaggtttcta tataactttg aatacctgtc tgatataacc tgtcgttcaa
     1201 attatacgct tttgccaaat gtttttgagc cgacgctgtt tgtttgagct tattggttaa
     1261 acgctcattt tctgataagg cattggtcgt atctcttaat gctttttgta aagtatcaat
     1321 ataattataa taataaacct tatttacgcc tttagcctta gctactgcac ctaaaacagg
     1381 catatcaata atgggggctt tcaacaattg atcattaaat ttcaccgttt ttttcggtaa
     1441 tgcgtagcgg ctatctccag ctgccaaacc accaatataa tctaattgta tagtaggtaa
     1501 aagattactt tctgcaacac caattccttc atgtgaagca cgcaaacgat tttcagctac
     1561 ttgcatatca ggccgatttt caagaactgt taacggtaaa gaaccgggta ttaatttacg
     1621 atttttaagt tgtaataatt ttttaactgt ttttatttcc cctggatttt ggtttagtaa
     1681 ataccggaga gcattgcggc ttataacgat attacgctct gttacctcga gttcaccttg
     1741 tatcaaattg acctggctat acaactcttc ctgctcaatt tcagaactta atcctccttg
     1801 gtaaacttta ctggcaacct ttgccaactg agttaaatca ttggctagtg tctgtaaaaa
     1861 ctctttgcgt tctatttctg caagataggt gaaataactt gccgttattt gagaaataat
     1921 tgttagtttg agagcatcgt cctctgcttt tacctgtgca agagtatatt ttgccctttt
     1981 ttgctctctt atttgattga tgatgtttat tgtatagtta ggaataaaaa caatcagaac
     2041 gccaggaaaa cccgttgccg gatttcttga ataaccagtc ataagatcga gggtaggcac
     2101 ccactgataa cgaatttttt taagctcacc ctctgccgct tcaatgctcc ctcttgacat
     2161 gccaaggctt gtattggaaa tcaatcctct ttcaatcaac tggttgagag tggggtcttt
     2221 aaaatcacgc caccaggcta tgtaaggaat attcttgcta tcgatatttt taacattttt
     2281 gtcttttacc gaccatttat taattacttc aacatggggt ctgtgataag gctcactata
     2341 gcatccgact aaaaagactg ataataaaat gattttaacc ggacgtagca tgctttactc
     2401 cctcactaag atcaattgtt tgctcccctg caacaaagtc ttttaagtgc accaaatctt
     2461 tttgaacctg ttgatatagt tgataataat caaacgtatg ataatgtttc gtataataag
     2521 ccatatataa ggctttcagg ccaatgaaaa tacgattagc caattccaca aactgattaa
     2581 attcatcccc gacagcatgt tctgaactga ttaaaaactg ggagtttgtt atgagcttat
     2641 tgacctcgaa caaactgtca gtcaatttga tacaagcctg gaaccattct gttttcttta
     2701 ttttaccctg atttagcaaa cgaaccagcc tgtaaagatc atcaatcact tcagcctgag
     2761 tacttttgat aaatcgctgc atcagtccgt aatgcctaaa ccaaaagtat tcaaagccta
     2821 gaataatagt aaaggcgatc aacgtgcaaa gagtataatc gataataaaa taggtgacag
     2881 ggagcgtatc ttccgtacta aagtaaccag aaccaagtac tgacacattg accgtaaata
     2941 cagtgggaac actgtatgct cttcctacca gaaaaaacgc gaaaaaaacc attaaaggca
     3001 taatcagaat taacgttcga taatccagat gacctacaaa ccataataga tatccggtaa
     3061 ataggcctaa aagcactcct aaaaaccgat gataagtcct gaatatagtt gttccattat
     3121 caaaaccggc ataaatcatc atgacagcaa agcccgtcca cccggcccgc ggataacgca
     3181 accattcctg gacaaaaata gtaaacgcaa agaccgtgct gatctgaata gagcgcagct
     3241 caacataaga aaatgcctgt acccctaacc atcggcgcat aagtaattcc agcttaaaat
     3301 taattgatac aaaatttcca actcatgcgt ttctttaaat gcaggcagct cctggtctga
     3361 gagtatcagt ggttgcctag ctttacagga ttgacaaaga cgttcaagat aatgatgtaa
     3421 aacaataacg tattccttac gcagttgctt ttgaaaagac agcaagtaag acatggttaa
     3481 gattaactca aaagataaaa tggttatctt taatatactg tagcacttaa tatttcttgg
     3541 cagcattttg gaataacgct ccatcacaat aatgcccgaa aaaatagcga tagttttgac
     3601 ttctcccttg caaatttttg cagataaaga ttccaggccg gtaatgactt cgagaaaagc
     3661 acgctcccaa atatcaaaat aatacttttt gggaaaaata aataatccag cgaagattac
     3721 cgccatggcg gccaaggtat ataaagcatt attaatagca atataaaaat tgctatctgc
     3781 ctccacacca taaatcaggg aataggacgc caaacttagc atcaatggaa caataacaag
     3841 cattttttta aaatggtgaa gtacgaggaa ataaagagcc aaggtataga aaaaaacgaa
     3901 aaaaataaaa agcacgttgt atacagaaat tatgccgaac aagaatatag agagtatgct
     3961 tcctaaaagg gtatagagta aaagcaagta cttctctttt agggttgttc ccgccgcctc
     4021 cacagtaaaa catgtgattg gggcataaaa ataatagaaa tacggattgt caatggaaga
     4081 aaaaaaataa aatataatca tcagttccaa aacaaatact gcttttagtc cattaatacg
     4141 atgttcttta tacctatcgt aggatgcgag cttatctttt agttgagata taaacataag
     4201 cactggcgcc aggatttaaa gggtagtcag gatcgggatc caatatcttg atttgtaatg
     4261 gaaatcgctg aggaagcaac agccattcat tttgatttgt aacaacctga atttgactct
     4321 ttggagcagt gctttgtcgt tcagcagccc ataacgagtt gacaacaacc ccatgaaata
     4381 ttttgtcaaa ataatacatg cgtaaaatga tatacgcttt atcaccttga cgtacattgc
     4441 gtaagtctgt ttcattaaaa ttagcctgga tataatagtt tgcagtatct ataaaagaaa
     4501 acaccggttt atggatctcg ataggggtat ttggagagac atacatatta tctatcactc
     4561 catcaccagg ggctctgaca atggttaaat caagattaac cttggcatta tccatttttg
     4621 ccttaaggct ggcgacagta tgtctttgtt gttcaatctt tgcttgcaaa accgccactt
     4681 cctgttgcag agcatttctt ttattgctta aagtagaaac atcataacca agatttttta
     4741 attcaagaac agatactgct tcggccacat tcttattttt tttaaggcca agagcaaatt
     4801 gagctttttc aagctcagcg tccgttgctt taatcaaagc aaccgttctt tcaatctgct
     4861 tttgaaatac gtctattttt tttaatcctt ccttgtaatc agccattgct tgttgataag
     4921 ccaactcata aggaaccctg taaacgctaa atataggatc gttcttcttt actttttggc
     4981 cattctttac atagatttct gtaataaaac cagatacatc agcagctaca ggagtcacat
     5041 tagtaacaac aaacgcattg tcagtgaaag ggagcaaata ggaaaataaa tgaaaaaggc
     5101 caataataag agccaagcca acgacaataa tgggaagatt gatgtaattt ctggcctttt
     5161 taaaaaacat agggatagaa taactaaaga aaaaccggat atgtttagtt actattttat
     5221 accatgtggc aaacttctct ttcacaatgc agcgcctctt tgatgatgac tttataatac
     5281 aacatcagtt tatctctcaa agagagaaac cccatgacaa ggccctccct ttttcggcat
     5341 taaaactaaa atacctcttt tttccgtttt cgaaacaatc tggaaagatt gttaggtgac
     5401 tcatataatc ggctttttcc atcctcaggt aaataacccc attgagattc ttctgccagc
     5461 acagccttgg catctgaata cccacattcc attcgcttct taataaccac tcggccaaag
     5521 ttgtaatcct tcgaagccgc ttttactatt cttgaactgt aagccaaatg aaccaaggta
     5581 caatgatgag ggcttccaat gtctaacaaa tgagcgctct ccttcttttg ttcatccgtt
     5641 aaaatacggc ttaattccat cattgagttt cgcatttttt gtctctgcaa ataattgtaa
     5701 atagtacgtt gggcatgggt tccatatgaa atgtcttttc ttctctcttc aatttcactc
     5761 atgcttttgg gaataaacga tcgcccacca aaacaatcaa ttagaaaaca caaggtattt
     5821 tctgcaggaa tggcctccag aataacttct aatggggtgt tagagtgcac accaccatcc
     5881 caatataact ctccatcgat attaactgca ggaaagcccg ggggtaaagc agcacttgcc
     5941 ataatgtgct ccacttctat acgataattg atattattaa aatagattaa atgaccattg
     6001 ctgatttgaa cagcaccgag acttaaacga atggggcagg aatttagcaa ttcaaaatca
     6061 ataagatcca gcaatgtctt gcgaagagga gtcgtatcat aatagcttag agtcgttgga
     6121 tcaccccata aaggaattgt tccggtgcac cattttggaa aaaagaactc aggctggcca
     6181 aataataaag cacaagcggc acttaattta ttataaaaat ctaaagtgat atcagattca
     6241 cctaaaaaat cgaaaaaatt acccggggca atttcatccc aaaatctttc gagtttttca
     6301 atacgctttt ctggaggatt tcctacaata atcgctgctt gtatggcacc aatagaggtt
     6361 gctgcaatcc agtccggttc atacccggct tcaagaagcc ctttaaccac accaaattga
     6421 taggctccca aagatccacc gccttgcagc acataagcca cacgggggaa acgtccttga
     6481 gccttgaggg gtaagttatc aaagcgattg gcataaggat tattaccgcg cggtttaggc
     6541 tccacttcca aactcataaa aactcctttt tcactttagt tgatcatccc atcctggctg
     6601 aaatgttggg aatacttgct gccgcttaat aacaccgttc aaaatcagat tgatatatac
     6661 attgttatta agtaacgtga gttatggatt acgattatat aagaagctgt cgatatcccg
     6721 ggttcgttcg ggctgaggag atgcgtaagc attgtctcga agccttggca agaaacatta
     6781 aattccatac aaggcttcga gacggcataa atgcctcctc agcccgaacg attcaagata
     6841 aagtatccat aactcacgtt aaataagttt cgcagaaatc ataagtgttt aatcattcac
     6901 attgtactca taatcctttg agcaaccaag ataatatatg aagatcattc cgaatagctt
     6961 taattatccg gctaaaatca acacaataag caactaaaaa tgtcctgtac gaaaatcatg
     7021 tattgcctgc ataatttctt cttgagtatt cattacaaaa ggaccatagc gaacaatagg
     7081 ttcatgaagt ctagccgcag ctatcagtaa acaatgattt ctattttcat tgcctttgcc
     7141 ttctatttgt attctttcac cgtttgtcag gctggctaaa gtccctgcgg taatttgctg
     7201 tcctcctgta ttcacagcac cttcaatgac aaaaaggaca gcttgataat catcaggaat
     7261 cgtttgttga atagttgcat tggcagggag ggtgatgtct agaaacaaag gtcgggtcgc
     7321 aatttcaaca atgggtgaac tcaatccttg atcaattctt cctgctatta ctttgatggt
     7381 tgcacctgat tccatgactt caataggtag ttgatccgat gatttttcct gataacgagg
     7441 ttctcgccat ttttctgcag caggcaaatt taaccacaac tgcaaaccat gcaaccggcc
     7501 cgttgacgac gaaggcattt ccgaatgaat aatccctttt cctgcagtca tccactgcac
     7561 atcacctgcg tttattactc ctttgttacc cctattgtct tcatgggtaa tgcttccctc
     7621 catcagatag gtaattgttt caaatccgcg atgcggatga ggcggaaacc cggcgagata
     7681 atccaatgga tctgaactgt caaaataatc aaaaagcagt ataggttcaa agtcattggt
     7741 tttatcagct cctatatagc gacgtaattt aactccccca ccttcgcgta ttaatgttcc
     7801 ctggattaac tgctctactt ctattgcttt catcttaatt actcattaat tactgtctta
     7861 tattaagata gttcaaaata gtatggtttc ctaattgaat tactattatt taaactattg
     7921 gcacgttcag ttatcaagga tatgatgaaa atagactcta aggataggag cagaatttat
     7981 gaacacaacg gaacatacag agctgggcaa tggtttgctc atggataaaa tcgaaggcaa
     8041 tccatactta cgaatagatg agtttggcgt gctccatctg cggcttcaac gattcggtga
     8101 aaacaacctg cctgaaccaa tggagctgga gctgtccgct ggcgaaatta ttgccatggc
     8161 gggtgactac tttacgcagg ctaattggac tatggatttg gatctaccca aatgcgagct
     8221 ctttaactcc ccggcagaat tgggaaaaca cctcattcgc aaacctatag atcctaaaga
     8281 agagaatgct ttaattacag catacaataa cctggctgct cctgatgtta ctcgaaaaga
     8341 aattgatcgt atttattcca ttaataatgc taactatgtg cccttttcgc ctacgttgaa
     8401 tttctatgct caacaactga tgtattattt tcgcgtcaaa gactatgggg aaatgcttgt
     8461 tcggaaccaa actcatttta cgccatggtc tattcgagtt tatatcttgg gtcacgctat
     8521 cgcattgaga tatgcacatt tatcctacga attaaaacaa ttggctaccg acagaaatta
     8581 tcaatcagat aaccctgact tgagtacttt aaaaatctcc ttacaaaata aaaatgaaac
     8641 tctttcaagc aataccctgc ttgatttggc gaatcgctat cacgcccaag cttattctat
     8701 agagctgttt acttttcact actattctga tcattttgct acaggacata tgtccatgat
     8761 tggcgattta cgtgtcgtat taaaagagcg tttcggagta tggggcaata tcctggctaa
     8821 taatcttcat gatgaagtaa atcgtgttgg agtttataca gtgagaccct atgatccaac
     8881 ccctaataca acagaggctc catctcgagc gcgtggagat ggaaaatttg atacctgcct
     8941 taatcaattt aaccgtttag cttgcctaaa cggtatgacc gcatcactaa aggacatcaa
     9001 tcaagtatta tcaggaagtg taataccgga gcaaaaaaat ttcggtggtt tggtgcatat
     9061 gcccgacgtt gattttaact caagacaaca tcaaccattg ctggttttaa gtaaagggaa
     9121 agtatattac cgcaacaatc tcagtcaaat tcacataatt tctccttctg aatatgaggc
     9181 actcagagaa aaccctgaaa aacatgggta taaagagcta accagtaaat gggctgcctt
     9241 taaattagtt accaaattac gcctcttccc ttatctttat gacggctcgg ttatgcctgt
     9301 ttctgatgaa aagctcgccg aaattatcgc agatgaaaaa tgcagaaatc cacatcgagc
     9361 tcccatcccc acaccatcat gcgttccgga aagcgagcca actgtattcg attggcgaac
     9421 aaaagcgagc tggcgtaata ataaagactc actggatatt ttggatgggt taaagaaaca
     9481 cagtattttg ggagcaaaac aagcccctgc ggccattcaa gaggaagaag aggttcagtt
     9541 gaatttggga gtctgattat ttcctgat
//
LOCUS       DBNCWK010000118         9448 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.118, whole genome shotgun sequence.
ACCESSION   DBNCWK010000118 DBNCWK010000000
VERSION     DBNCWK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.118"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            203..1069
                     /locus_tag="AC06IO_002331"
     CDS             203..1069
                     /locus_tag="AC06IO_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HIO9875505.1"
                     /translation="MKPGYLLGALLLSPILAFANSDDNADGLIFSPLPQNKNTVVRHY
                     SNEQEMPNLSQMAQRTIDFPTQIVRVSGNLTGLELSCDDVENEIDQVFSKKISPNLFT
                     YNTYVSCGYDVNDPEQHATNFSIQSYFDPLTDNAVDYLKSYLKEYNGYNLFNTTTLQI
                     ENAKGIIVSMNLNAGLKSNPDKTPFTLYRQDRNNFYFKSNFDVRKELISDIYQRFYSN
                     DPDMILPFFDKWIFSYAGSVYYSILMASNYLELQPERIFVMENEGDIFVSDLRYYFAN
                     LCMKRNPNKHCL"
     gene            complement(1209..2270)
                     /locus_tag="AC06IO_002332"
     CDS             complement(1209..2270)
                     /locus_tag="AC06IO_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIO9875506.1"
                     /translation="MLKGLKVIDLSSVLAGPSVGTFFAELGASVVKIEHPVHKDVTRT
                     WKLPGEDPHSEISAYFASVNFGKDYLFLDLMKEEDYLCFLNLIAEADILIMNFKKGDD
                     IKLRVQDDHLHSINPRLIIGKINGYGEESDRVAYDLVLQGESGFMSINGTQDSGPVKM
                     PVALIDVLAAHHLKEAILLALYHREKNGKGRVVSVSLYDAAVSSLVNQASNYLMTGQV
                     PQRIGSLHPNIAPYGKLFQTSDGALIVLAIGSDRHFVKLCNILGLKDLPGKDEFRHNQ
                     NRVRNREQLAALIAEQTEKLQSNELLEMLHRENIPAGKIMDLKDVFDNERAKSLIRKE
                     VIDDVPTARVTSVVFKQTL"
     gene            complement(2408..3817)
                     /locus_tag="AC06IO_002333"
     CDS             complement(2408..3817)
                     /locus_tag="AC06IO_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIO9875507.1"
                     /translation="MISKKITQISYSIVLALIVGVAEAKTTRPADEQQLARSIYKQFI
                     EVQSGFTTGSTTPLVKVAVKYLKKAGFSNEDIFVGGASPQKANLVVRYRGTGEKKPLL
                     LLAHTDVVEAKASDWSMNPFQLTEKDGYFYGRGTLDDKAQAAIWIANLIQYKQEGFKP
                     KRDIIVALTADEEGSSPYNGISWLIKNHKDLIEADFALNEGGWGDLANGKKISQNIQV
                     SEKYIVNYNLEVRNKGGHSSLPTKDNAIYRLAGALERLSKFNFPLKTNDVTAAYFKQM
                     AAIETGPLKTEMVEASKGSKQAMQRLADSSPQWNAILRTTCTPTLLEGGHAMNALPQL
                     AAVTINCRVLPEDSPEMVEQSLKTAINDPGVTLKRIGKLSRGPSSPLSPEILKTITHL
                     TQRYWPEVPAIPIMVTGATDGRYLRSAGIPTYGVMGLFLDRDDFRAHGRDERISVESF
                     YEAHAFLYDLVKQLSSNVT"
     gene            complement(4076..5554)
                     /locus_tag="AC06IO_002334"
     CDS             complement(4076..5554)
                     /locus_tag="AC06IO_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9875508.1"
                     /translation="MNIAIIGGGTGITTAWALENQQKFHVTVFEENDRLGGHIHSIKI
                     NNVILEGGAEFIGPPALYPNVHQLFQYLGVALDQFELNMDFDNLKQRDHIVLPPLYHT
                     SSGKSEDTSSILYTLSCGLFGKRRKQNETRVSVDTMLTEMYNLLEMNHLIMDARNKLL
                     HPDNLITLEQFIKEFKEKCVHALSPIDKFAEEFLYPLIASGWGVSIDTIKTFGAHYAM
                     NYLAAGMNWFDAPEGLSSYIDRMVTQCTNTQFNANTAIKKLIPITVDGQIKYQLLKRD
                     NTFVCDAAGSPMIYDDVVISTPAYVTSELLSGIESNTIKVLREKLANVTYYDTTVVFH
                     QDPEYLSPYRTVVHSRFDGTQSANTMCKLWKFNKEETPIMKTWVLPGQAMPKNVLREV
                     HYKHPVMDKNYYEAQQALHAAQGEAGLWHGGILAGFNDSHESGVTAALQVAAQLNHRE
                     HCLNKNERLALFPHILNSVLSPKLSTDIRMEVAGVAAYPSIP"
     gene            5921..6352
                     /locus_tag="AC06IO_002335"
     CDS             5921..6352
                     /locus_tag="AC06IO_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875509.1"
                     /translation="MKKSSFFDYSFFTTSDTKEEFPLFPEEGMDCLFEKLGSRITSKK
                     AGNTYFITPTDPSIAIQKGNDGVHEYYHLLLNKKVIAVSSFVNHKTTIYLANIIKQLE
                     PERIGTPVDFDFLGDTDETDSVFSDTLDNTEGNIDQMPSPR"
     gene            complement(6399..7310)
                     /locus_tag="AC06IO_002336"
     CDS             complement(6399..7310)
                     /locus_tag="AC06IO_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875510.1"
                     /translation="MYSKYPVFFPNKNIKSFSGVRFSNVVGIPSAIESLYRGDNNLAG
                     IIFLLPTLITGVFCQNFPEVVDMEQVRLHKLTNLSNDFHMVSMSEDPQIALDWGKGCF
                     ITIDPVLFSDYIVDVHATFRKNQLNFPDRMEQEREHIALVVPFCCIKKITIHNKELAN
                     PFYLSIPQDNHEARMEFNTLYSELISLLRKKYTQEVDGEEEKIALRAYAIRYLDFYSK
                     FGSCNNPFNKSLAQLVELYPEFMSNFLQSSHLSSKTGLMKDILLNSSDNLFKEHPYTQ
                     SIDASYIFRVKESTTCYEDDWAKPVYD"
     gene            7519..8361
                     /gene="htpX"
                     /locus_tag="AC06IO_002337"
     CDS             7519..8361
                     /gene="htpX"
                     /locus_tag="AC06IO_002337"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIO9875511.1"
                     /translation="MINNLKTFILLASLTALLVVIGGLLGGSTGMLVALLFAGIMNFS
                     AYWYSDTLVLKMYNAEPLSNNHFVYHIVSELAHRAGTSVPKVYLINNSTPNAFATGRN
                     PENASIAVTTGLLDRLTQEEITGVLAHELAHVIHRDTLINVVSATIAGAISGIANMFM
                     WLSMFGHNSNNEEGVHPVVGMIMMIVAPLAAGLIQMAISRSREFEADAGGARISGNPQ
                     WLASALLKLDQANHEQYFDEAETHPATAHLFIINPLNGEKLANLFSTHPSTAERVARL
                     RAMY"
     gene            complement(8536..9354)
                     /locus_tag="AC06IO_002338"
     CDS             complement(8536..9354)
                     /locus_tag="AC06IO_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875512.1"
                     /translation="MFIKSPCIDLTRHSKIWINPDGEIPKKIVERLKWQKETRPRDAI
                     TLFVNRACEDKSNSAVESLRACGVKIKIIELCLEKNEKQDDPFIIACFNKALALAKKE
                     KNLVDRVRASVRATNVLRLMKLVQHEGLYSDNDVLFLKFEATRLLSPYLFGQYEGDVN
                     DVHLFGVAINAPLTTDYFYTRLVEKMKKPWEEEITPDEFEPPCGLYLIPDEIISKIQF
                     GHLKFAEIRDCIITGSDQSHHDITRAKKLLNFEEDSLLDEAKSIVASQEKQYRM"
BASE COUNT         2869 a         1911 c         1679 g         2989 t
ORIGIN      
        1 ttttaaagta aaagaaattt gctattctct gtcgctcaca tgcgatcatc gcatctgttt
       61 tatttatgct ggttatagga tctaatcttc aaaccagata agatatactg tctgtcccat
      121 cggattagaa caattattaa acaatattct aatcggataa tcatttgaat ggagttctga
      181 attaatttaa aaggagtttg ttatgaaacc aggttattta ttaggcgctt tgcttttatc
      241 tcccattctc gcttttgcca attcggatga caatgctgat ggccttattt tttcgccatt
      301 gccgcagaat aaaaataccg tagtccggca ctatagcaat gaacaggaaa tgcctaatct
      361 tagtcaaatg gctcaaagaa ctattgattt tccaactcaa attgttcgag tcagcggtaa
      421 tcttacaggg ttggaactga gctgtgatga tgtagaaaat gaaatagatc aagttttttc
      481 aaaaaaaatc tcccctaatc tgtttactta caacacttac gtcagctgtg gctatgatgt
      541 caatgatccg gaacagcacg cgacaaactt ctctattcaa agttatttcg atccattgac
      601 agataacgct gttgattatt taaaatctta tttgaaagaa tataatggat acaatttatt
      661 caatacgaca acattacaaa ttgaaaatgc caaaggcatc attgtatcga tgaatcttaa
      721 tgccggttta aaaagtaacc ccgataagac accattcacg ctttatcgtc aggataggaa
      781 caatttttat tttaaaagca attttgacgt gagaaaggaa ttaattagcg atatttatca
      841 acgattttat tctaacgatc ctgatatgat tctacccttt ttcgacaaat ggattttctc
      901 ctatgccgga tcagtttatt actccatcct tatggcatcc aattaccttg aattgcaacc
      961 tgaaagaatt ttcgtaatgg aaaatgaagg ggatatcttc gtatccgatt tacggtatta
     1021 ctttgccaat ctttgtatga agagaaatcc taataaacat tgtttgtaac taatgaaatc
     1081 agcagaaacc gttgcgcgta tgctttctaa tgtgcgctgc agaaacgttt gcagcgcagc
     1141 aatgaagaga cagcttatgc atgaggacta atattcttca tctgcataat tcaatggctc
     1201 ccctttgatt ataaagtctg tttaaaaaca acggatgtaa cgcgcgctgt gggaacatca
     1261 tcaatgactt ccttgcgaat gagggatttc gcacgctcat tatcgaaaac gtccttgaga
     1321 tccataattt tgccagcagg aatattttcc ctgtgcaaca tttcgagaag ctcattgctc
     1381 tggagtttct ctgtttgttc agcaattagt gcagcgagtt gctcccgatt cctcaccctg
     1441 ttttgattgt gccggaattc gtcttttcct ggtaaatctt ttagcccaag gatattgcat
     1501 agttttacaa aatgccgatc cgaccctatt gcaagaacga taagcgcccc atcagatgtc
     1561 tggaacaatt tgccataggg ggcaatattg ggatgcagtg atccgatgcg ctgcggcacc
     1621 tgcccagtca tcaggtaatt cgatgcctga ttgacaagac tgctaacagc agcatcataa
     1681 agtgaaacag agacaaccct gcctttccca tttttttccc gatgatagag agccagaagg
     1741 atggcttctt tcaggtgatg ggctgcaagg acatcaatta gggcgacggg catttttacc
     1801 gggccgctgt cctgtgtccc attgattgac ataaaaccgc tttcaccctg aagaacaaga
     1861 tcataggcaa cgcgatcgct ttcttcaccg tagccattga tttttccaat gataaggcgc
     1921 ggattaatac tgtgtagatg atcatcttgt acacgcagct ttatgtcatc tcccttttta
     1981 aaattcataa ttaaaatatc tgcttctgca ataaggttaa ggaaacagag ataatcctct
     2041 tccttcatga gatctaaaaa caaatagtcc tttccaaaat taacactggc aaaataagct
     2101 gaaatttcag aatgaggatc ctctccaggg agtttccatg ttcttgtcac atccttatgt
     2161 acgggatgtt cgattttaac aactgatgca cctaactcag caaaaaaagt acctacagag
     2221 gggcctgcaa gaaccgaact cagatcaatg acctttaagc cctttaacat atacctgttt
     2281 tccaaatctc aataaacatt cgttgttttt acgagtttgc acattgggaa attatgcaga
     2341 actgaccaaa gggacctcaa atcaatatcc tcccgattta gcgaagtccc aactggaaaa
     2401 accattctta agttacattg cttgacagtt gtttcactaa atcataaagg aatgcatgcg
     2461 cttcataaaa tgactcaaca ctaatccgtt catcacggcc atgggctcgg aaatcatcgc
     2521 gatctaaaaa tagccccatc actccatagg taggaattcc agctgatctc agataacgtc
     2581 catccgttgc gccagtaacc ataatcggaa tggctgggac ttcaggccaa tacctttgag
     2641 ttagatgagt aattgttttt agtatttccg ggctaagtgg agaacttggg cctctggaca
     2701 atttgccaat tcgctttagt gtaacccctg gatcgttaat agctgtcttc aaagattgct
     2761 caaccatttc aggtgaatct tctgggagca cgcgacaatt tatggtaaca gccgctaatt
     2821 gtggtaaagc attcattgca tgccctcctt caagtaaggt aggggtacag gttgttcgta
     2881 aaatagcatt ccattgcggt gaactatcag ctaaacgttg catagcttgc ttcgagcctt
     2941 tgcttgcttc aaccatttct gttttcagag gccctgtctc aatagccgcc atttgtttaa
     3001 aataggccgc ggtgacatca ttggttttta aaggaaaatt aaattttgac agtcgctcta
     3061 aagccccggc aagacgataa atggcattat ctttggttgg tagcgaacta tgcccccctt
     3121 tattacggac ctcaagattg taattgacaa tgtatttttc gctcacctgg atattttgtg
     3181 atattttttt cccattggcc aaatcacccc atccaccctc attaagagca aaatctgcct
     3241 caattaaatc cttgtgattc ttaatcagcc aactaatccc gttgtatggg ctgcttcctt
     3301 cttcatcagc cgtcaaagca acaatgatat cgcgtttggg tttaaatcct tcctgcttat
     3361 attggattaa gttggctatc caaatagccg cttgcgcttt gtcatccaaa gtgcctctgc
     3421 cataaaaata cccatctttt tcagtcagtt ggaaaggatt catgctccaa tcagaagctt
     3481 tggcttcaac aacgtccgta tgcgcaagta ataacaaggg tttcttctcc ccagtaccac
     3541 gataacgtac aaccagattg gctttttgtg gactggctcc accaacgaaa atgtcctcgt
     3601 tagaaaaacc ggcttttttt aagtatttta cagccacctt aaccagtggc gttgttgaac
     3661 cggtagtaaa tcctgattgc acttcgataa attgtttgta aatagagcgg gcgagttgtt
     3721 gttcatccgc agggcgagta gtcttcgctt ctgccacccc aacgatcaga gccagaacaa
     3781 tggaatatga aatttgagtt atttttttcg aaatcataaa atgaaatcca ttcaaagcat
     3841 gacgcgttta aagagaaatc cactataaag taatctgagt aaaaatcaat gtgcatttgg
     3901 acccaggact tgaattttgg atgacaactg tacattttct ttgatcggca gagtttaatt
     3961 cttacctcca tttgcacagc aaaatttaaa atcaaattca caattaccgc cctcaagttg
     4021 tatctgagag cgatatcaat ttatagatat tgtcaaaaaa taaccgatgt caggtttatg
     4081 ggattgaagg ataggcggct actcctgcaa cctccatcct tatatctgta gacaattttg
     4141 gagataggac agagttaagt atatgaggga atagcgcgag acgttcattt ttatttaaac
     4201 aatgttcacg gtgattcaac tgcgcagcaa cttgtaatgc tgctgtaacc ccactttcat
     4261 gtgaatcatt aaatccagca agaataccac catgccataa cccagcctca ccttgagcgg
     4321 catgtaaggc ctgctgtgct tcatagtaat ttttatccat gaccgggtgt ttataatgaa
     4381 cttctctcag tacgttttta ggcattgctt gccctggcaa aacccaggtt ttcattatag
     4441 gggtttcttc tttattaaat ttccagagtt tgcacatggt atttgctgat tgagtaccat
     4501 caaatcggct gtgaacaaca gtcctataag gagacaggta ttcgggatcc tgatgaaata
     4561 ctactgtcgt atcataataa gtgacattgg caagtttttc acgcaatact tttatcgtgt
     4621 tactttcaat accagacagc agttcactcg ttacataagc aggtgtggag atgactacat
     4681 catcataaat cattgggctt ccagcggcat cacaaacgaa tgtattatct cttttcaata
     4741 attgatattt tatttgtcca tcaactgtta ttggtattaa tttttttata gccgtattag
     4801 cgttaaattg agtgttggtg cattgcgtca ccattctatc aatatagctg ctcaaacctt
     4861 ctggcgcatc aaaccaattc attcctgcag ctagatagtt catggcataa tgggcaccaa
     4921 atgttttaat tgtatcaatt gatactcccc atcctgatgc aatcaaagga tacaagaact
     4981 cctcagcaaa cttgtctata ggtgaaagtg cgtgtacaca tttctcttta aactctttta
     5041 taaattgctc taatgttatt aaattatctg gatgcagtaa tttgttcctg gcatccataa
     5101 ttaagtggtt catctcaagc aagttgtaca tttctgtgag catggtatcc actgaaacac
     5161 gggtttcatt ctgtttcctt ctcttgccaa ataaaccaca ggataatgta tatagaattg
     5221 atgaagtgtc ctcagatttg ccacttgatg tatgatacaa aggcggtaac acaatatgat
     5281 ccctttgttt cagattgtca aaatccatat ttaactcaaa ttgatctaaa gccaccccca
     5341 gatattgaaa caattgatgc acattgggat acaaggcagg tggtcctatg aattctgctc
     5401 ccccttccag gattacatta ttaatcttga tgctgtgaat atgcccgcct aaacgatcat
     5461 tttcctcaaa tacagtcaca tgaaattttt gttgattttc caaagcccag gcagtagtta
     5521 ttcctgttcc accaccaatt atcgcaatat tcattacttt tttagtcatt ttaaaagtcc
     5581 atttccaaat accaggctcg taaactaaat ggttagaata aaaaagtaaa taagtccttt
     5641 gtgattaatc gaaaaagacg gaggaggaag attattaaaa acaatgcaca tgcacttgtt
     5701 tatgttcaat ttacgactac cgcttcattt caaataactt tataacgcac gctcatcgtg
     5761 aattactaca aaaatttgcc tcaacgttat tactctattt gctactcaag tatactcttc
     5821 catttcttat attagcgtcg gtggttagcg attcaaatac ctttaaatcg tttatcatga
     5881 tataattttg agcatattta tgtttaatgg tagctcctat atgaaaaaat catctttttt
     5941 tgattattcc ttttttacca cttcagatac caaagaggaa tttcctcttt ttcctgaaga
     6001 aggcatggat tgtttatttg aaaaactagg ttcacgcatt acatctaaaa aagccggcaa
     6061 tacttatttt attacgccca ctgatccatc tattgctatt caaaaaggta acgatggggt
     6121 tcatgaatat tatcacctgt tacttaataa aaaagtcatt gctgtatcat cattcgttaa
     6181 tcacaaaacc acaatttatt tagctaatat cattaaacaa ctggaaccag aacgcatagg
     6241 tacgcctgta gattttgatt ttttgggtga tacagatgaa acagattccg tgttttcaga
     6301 tactttggat aatacagaag gtaacataga tcaaatgccc tcacctcgtt agtagtcatc
     6361 ttcaacgaat tcaaggaaaa ccagcatctt tcttattact aatcataaac cggttttgcc
     6421 cagtcatctt cataacaggt agtgctctct ttaaccctaa agatatagga agcatcaatc
     6481 gattgagtat aaggatgttc tttaaataaa ttatctgatg agttcagaag aatatctttc
     6541 atcaaacctg tttttgatga aaggtgactt gattgtaaaa aattgctcat aaattccgga
     6601 tagagttcaa ccaactgagc aaggctttta ttaaatggat tgttacaact gccgaattta
     6661 gaataaaagt ctaaataacg tatggcgtaa gcacgcaatg cgattttctc ttcttcccca
     6721 tctacttcct gagtgtattt ttttcttaaa agtgaaatta attcgctgta gagcgtatta
     6781 aactccattc ttgcttcatg attatcttgg ggtatgctga gataaaaagg gttagctaat
     6841 tctttattgt gaattgtaat tttttttatg cagcaaaaag gtacaactaa agctatatgc
     6901 tctctttctt gctccattcg gtcaggaaaa ttgagctgat ttttacgaaa agtggcatga
     6961 acatctacaa tgtaatcaga aaataaaaca gggtcgattg taataaaaca tcctttcccc
     7021 caatccagag caatttgagg atcctctgac atactcacca tatgaaaatc attagataag
     7081 ttagttaatt tgtgcaatct tacttgctcc atatccacaa cttcaggaaa attttgacaa
     7141 aacactcctg taattagagt aggcaaaaga aaaattatgc cagcaagatt attatcacct
     7201 cgatataatg attcaattgc agagggaatt cctacaacat ttgaaaaccg cacacctgag
     7261 aaagacttta tattcttatt tgggaaaaat actggatatt tactatacat aagatgttta
     7321 atgacatata aatttgcgca acaatatacc acattgatct caaaataaaa ataaccgtgt
     7381 attacgctca atgggttttc ttgatctcca gctctaaagc atggacataa acagaaataa
     7441 cgctctaagt tgaattttta acatctggcc ttatatatga gatataaaat atagaaagag
     7501 taaatgaggt gatgagagat gataaataac ctgaaaactt ttatcttgct agcctctcta
     7561 acagcattgc tagttgttat tgggggattg cttggcggtt ctacaggaat gctggttgcg
     7621 ttactattcg caggaattat gaatttttca gcttactggt attcagatac tcttgttttg
     7681 aaaatgtata atgctgagcc tctttctaat aatcactttg tttaccatat tgtttcagag
     7741 cttgctcata gagcaggaac ctcagtaccc aaagtttatt taattaataa ctctactcca
     7801 aacgcgtttg caacagggag aaatccagag aatgccagta tcgctgttac aacaggattg
     7861 ttagataggc tcactcaaga agaaatcaca ggagttttag ctcatgaact ggcccatgta
     7921 atccaccgcg acactttaat taatgtggta agcgcaacaa tagcaggggc gattagcggg
     7981 attgccaata tgttcatgtg gctttcaatg tttggccaca attcaaataa tgaagaaggt
     8041 gtccacccag tggttggcat gatcatgatg atagtagctc ccctggcggc tggactgatt
     8101 caaatggcta tatcgcgctc cagggagttt gaagcggatg ccggtggagc acgaatttcc
     8161 gggaatccgc agtggctggc cagtgccttg ttaaagcttg atcaagccaa ccatgagcaa
     8221 tactttgatg aagcagaaac tcaccctgcc actgcacacc tgtttatcat taatccatta
     8281 aatggagaaa agctggcgaa cctgttttca actcatccct caacagcaga acgggtagcg
     8341 aggctaagag ccatgtacta attgaggttt ctctcattga ttcggaataa gtatcgcttt
     8401 aatcacaata ctaaacatgt caaggagatt gacttttctg aaacacttca gcaaacaatc
     8461 aaagactaac tacacaaaat aaagcctttg cattatttta tttggctaaa agttagatag
     8521 cgagcgtccg tatcatcaca ttctatactg cttctcttgc gaagctacta tgcttttagc
     8581 ttcatccagc aagctatctt cttcgaaatt taataatttt tttgcacgcg taatatcatg
     8641 gtgagattga tcacttcctg taataataca atctcttatc tctgcaaatt ttaaatgtcc
     8701 aaactggatt tttgatatta tttcatctgg aataagataa agaccacatg gcggttcaaa
     8761 ctcatcaggg gtaatttctt cctcccatgg ctttttcatt ttttcaacta gccgggtata
     8821 aaagtaatcc gtagttaggg gggcatttat agccacacca aagagatgaa catcatttac
     8881 atctccttca tattgaccaa aaaggtacgg agacagtagt ctggtagcct caaattttaa
     8941 aaataaaaca tcattatctg aatacaagcc ttcatgttgc actaacttca taagccttaa
     9001 aacgtttgtt gcccgaacag atgccctgac tcgatccaca agatttttct cttttttagc
     9061 tagagccaac gctttattaa aacaagcaat tataaatgga tcatcttgtt tttcattttt
     9121 ctctaaacat aattcaatta tttttatttt aactccacac gctcttaatg attccactgc
     9181 agaattggat ttatcttcac aagccctatt tacaaataag gtaatcgcat ccctcggccg
     9241 agtttctttt tgccacttta atctctctac aattttctta ggtatctctc catcaggatt
     9301 gatccatatt tttgagtgcc tggttaaatc aatacatgga gatttgataa acatagtact
     9361 atatcctctt tatacccagt gtctctcaat aaaacgatta tttttaggat ttatcaggct
     9421 caaaataaag cagcgcactg aaaataat
//
LOCUS       DBNCWK010000119         9305 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.119, whole genome shotgun sequence.
ACCESSION   DBNCWK010000119 DBNCWK010000000
VERSION     DBNCWK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9305
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.119"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..244)
                     /locus_tag="AC06IO_002339"
     CDS             complement(<1..244)
                     /locus_tag="AC06IO_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIO9875513.1"
                     /translation="MSVEIQQASSTNPNITFSESAIKHLVTYLEKNPGNKGVRLSVKK
                     TGCSGLSYVVDYVKEPSEGDMIASLSKDYLVCVDKTS"
     gene            complement(249..632)
                     /locus_tag="AC06IO_002340"
     CDS             complement(249..632)
                     /locus_tag="AC06IO_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIO9875514.1"
                     /translation="MMYNKIVQDYFFQPQHIGKLDLNDPFVIGVQSQPINQLNTVHFY
                     IKCDGDKLISKARFKTNGNPYVIAAMEWICCQIEGKTLESLPLIDFQILINVLQITNN
                     QYPVAVQVEDIYKEALTLMRKKFER"
     gene            complement(629..1762)
                     /locus_tag="AC06IO_002341"
     CDS             complement(629..1762)
                     /locus_tag="AC06IO_002341"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIO9875515.1"
                     /translation="MATTPVDPRVVEQMIKYLGPEGDFGNPASATHEYGRVASMAVEQ
                     ARSQIAETINASPQEIVFTSGATEADNLAILGAARFYKNKGMHLVTMSTEHKAVLDSF
                     HQLEKEGFQVTYLNPESDGLLDLGKLESALRLDTILVSVMHVNNEIGVIQDIASIGEL
                     LRNRGIIFHVDAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGALYVRHKPRIRLQ
                     PLSYGGGHEGGLRSGTLPTHQIVGMGEAFAIAEADRIPEQTRILNLRKQLWDGIRHLP
                     AIKLNGHEHRRIAGNLNVSFVGLNGDSLLFALSELAISTTSACSSASIQPSYVLRAIG
                     LTDTEAQSTIRLSIGRFTSEVQIKKAIDIICTQVSRLHELSPL"
     gene            complement(1767..2540)
                     /gene="trmJ"
                     /locus_tag="AC06IO_002342"
     CDS             complement(1767..2540)
                     /gene="trmJ"
                     /locus_tag="AC06IO_002342"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HIO9875516.1"
                     /translation="MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPD
                     LKAKEMAAGADDVLDAAVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELIN
                     QQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
                     LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRIN
                     LEKMEVSILRGMLSQVQKSLEWARKDNRSNKIGNKTDLF"
     gene            2656..3441
                     /locus_tag="AC06IO_002343"
     CDS             2656..3441
                     /locus_tag="AC06IO_002343"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIO9875517.1"
                     /translation="MEPLLNIAVNAARQAGEIINRYVEQVDRLKITPKNSHEYFSEVD
                     IKAEQVIINTIHKAYPEHGILAEESGIQQSDSDTVWIIDPLDGTSNYLHGFPFYSVSI
                     ALKIKNRLEHGVIYDPLRHECFAASRGRGARLNDRRIRVSKQTQLNASLLGTGFHFRD
                     ATLAQRYLPTFEALIGKCAGVRRTGSAALDLAYVASGRLDGFWEFGLRPWDIAAGALL
                     VREAGGLISDVQGGEDFLNYGDVVAGTPKVFKSLLQTISPVLK"
     gene            complement(3554..4510)
                     /locus_tag="AC06IO_002344"
     CDS             complement(3554..4510)
                     /locus_tag="AC06IO_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIO9875518.1"
                     /translation="MNNESSSNSNLDSQALLNQLVIDYLQERKRKRRWKWIIRAIILV
                     VLVYSSYKIVSVAKTTEASKDKEHIGLIDINGEIADSSSASADNFTKGIEKAYKSKGL
                     KALIVRINSPGGSPVQAEYIYNVLQYYKSLKPNIKTYAVCVDMCASAAYYVAVGADEI
                     YASPASMVGSIGVLYNGFGFVDALQKLGISRRLQTSGVNKAFLDPFSPTTEFQKEKLQ
                     TMLDIVHKQFITRVKEGRGNRLHVDDETFSGLFWTGEQALANGLIDGYASSGQLAREV
                     IKITDIVDYTYKQNVFDRLTKNLGTAMADELPVILGMKPGLR"
     gene            4599..7175
                     /gene="clpB"
                     /locus_tag="AC06IO_002345"
     CDS             4599..7175
                     /gene="clpB"
                     /locus_tag="AC06IO_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIO9875519.1"
                     /translation="MRMDKLTSKFQMALADAQSLALGRDNGFIEPEHLMKALLDQQGG
                     SCRPLLSKAGVNIPLLRTLIDQALDKLPKVSGTGGDIHISNALNRLLNLTDKLSQQRK
                     DNFISSELFVLAAINEDSNLAKILKQAGGDNKAIEKAIDELRGGETVNDPNAEEQRQA
                     LEKYTLDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKNKRLLALDMGALIAGAKYRGEFEERLKGVLNDLAKQEGQIILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVDEPSVEDTIAILRGLKERYEVHHGVEITDPALVAAAMLSHRYISDRQLPDKAID
                     LIDEAASLIRMEIDSKPESMDKLERRLIQLKIEREALKKENDEASKKRLVDLQKSIDE
                     LEQNYSDLEEIWKAEKATMQGSTQIKEALEQAKLEMETARRAGDLSRMSELQYGRIPE
                     LEKRLSQVSSVDAMETKLVRNKVTEDEIAEVVSKWTGIPVSKMMEGEKEKLLKMEEAL
                     HSRLIGQNEAVDAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALASFLFD
                     TEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HTDVFNILLQVMDDGRLTDGQGRTVDFRNTVIVMTSNLGSQLIQEMSSKFNYDEIKAA
                     VMDLVSQHFRPEFINRIDESVVFHSLTKEQIAKIAAIQINYLHHRLKQQNITLEVTSE
                     ALSHLAEAGFDPVYGARPLKRTIQQKLENPLAQSLLTGKFKSGDTIIVSYKDGVMEFS
                     KQ"
     gene            complement(7267..8580)
                     /locus_tag="AC06IO_002346"
     CDS             complement(7267..8580)
                     /locus_tag="AC06IO_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1751 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875520.1"
                     /translation="MTFTPPEFKILSVNTRNLETIFSTLLGRYKIITDPPVSEAVSSG
                     EIIRNTLETLLARTHKVVICKTDRETARDVFKQLSNELREVLKENNEEKNKQAILFLL
                     GALLHRYFRLIKEYDNFNSYIPVPSFFFKYKAPSDVKDCRLFQAIRLALGLPEVMEKN
                     YRINDLKILDVTTIVTALETFRDNMQLIVGKDEGKMPRYKSYPHFAADKNFETYLQEI
                     IDEHKRRNPVVLNQFKAINFIQSLVKQIEEEQRQIEEALTHLGKLLPKTCSDFKTISL
                     ELMEEQIKAQIESNVLQEKIIDLLYTGHIQENFSTMDCGSFIEAMKNCNNSLARYRAL
                     GGYCLLLQNEGVKEQLRFCIHQALGVEINPNELTDKDMLDAIRLLKTYFEANPKVELN
                     FDFFNGKGSMNTFILQTELALAKKVQTVNKAQEDNSETRTTSLFV"
     gene            complement(8593..9240)
                     /locus_tag="AC06IO_002347"
     CDS             complement(8593..9240)
                     /locus_tag="AC06IO_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1752 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875521.1"
                     /translation="MRFLRVGPCGRETMLEFAEFQQNALKLTDQLKKRSIPSLGCFCF
                     FSVILPNTAAIEDTHTIRLVAYSLDSENDDDLIAYLKGVTRYSAVTPKNINEFKKKVL
                     TGIYLMMWSHYSSTVSNLMNSTLVELFQRDLQVSSPQKMSDTLFDTSLEALSQYCSFA
                     YQNRNERIYDNLIRRLGKTVQADIHALRHSREKQETSFYDIYKGIMRTLGLNSVF"
BASE COUNT         2908 a         1844 c         1744 g         2809 t
ORIGIN      
        1 aacttgtttt atcaacgcat accagataat ctttagataa agaagctatc atatctcctt
       61 ctgaaggttc ttttacataa tccactacat aagataatcc cgagcatccc gtctttttta
      121 cagacaatct tactcccttg tttcctgggt ttttctccag ataggtgacc aggtgcttta
      181 ttgccgattc actgaaagtg atgttggggt tcgtactgct cgcttgttgt atttctacac
      241 tcattgcttt acctctcaaa tttcttacgc atcaaagtca atgcttcttt atatatgtcc
      301 tctacctgga ctgctacagg atactgatta tttgttattt gtaatacatt aattagaatc
      361 tgaaaatcta ttaatggaag tgattccaaa gtcttacctt caatctggca acatatccat
      421 tccattgccg caataacata agggttacca ttcgttttaa atctggcttt ggatatcagt
      481 ttatctccgt cacattttat ataaaaatgc actgtattca gttgattaat cggttgactt
      541 tgtacaccta ttacaaaagg atcatttaaa tccagcttac caatatgttg tggctgaaaa
      601 aaataatcct gtacaatttt attatacatc ataacggcga taattcgtgc agccgactca
      661 cttgtgtgca aattatatca attgccttct ttatttgcac ctcagaagtg aagcgtccta
      721 tggataaacg aatagttgat tgagcctctg tatcagtcaa accaattgcc cttaagacat
      781 aagatggttg aatactggca gaagaacagg cagaagtagt tgatatcgct aactcactta
      841 aagcaaacag caatgaatct ccatttaatc caacaaaact cacattcaga tttccagcga
      901 tacgcctgtg ctcatgtcca ttcagtttaa tagcaggcaa atgtcgtatt ccgtcccata
      961 gttgtttgcg taaatttaga atacgcgtct gttcaggaat tctatcagcc tctgcaatag
     1021 caaaagcctc acccatgccg acaatttgat gggtaggtaa tgttcctgag cgcaatccac
     1081 cctcatgccc gccaccgtaa cttaaaggct gtaaacgaat ccttggctta tgcctgacat
     1141 acagagcccc tacaccttta ggcccataat ttttatgagc agagaatgac atcaaatcaa
     1201 ctgaaagctg gcttaaatcg attggcaatt taccagcact ttgagccgcg tcaacatgaa
     1261 atataattcc cctatttcgt aatagctcac caatagatgc aatatcttgg atgacaccaa
     1321 tttcattatt aacatgcatg acagaaacca gaatagtatc taaccttaga gcagattcca
     1381 gttttccaag atccaacaaa ccatctgatt ccggattaag gtaggtgact tgaaaacctt
     1441 ctttttccag ttggtgaaaa ctgtcaagca cagctttgtg ttcagtgctc atagtaacca
     1501 gatgcattcc cttattttta taaaaccggg cagctcctaa aatcgccaaa ttatccgctt
     1561 ctgtagctcc tgatgtaaac actatttctt ggggtgaggc attaatagtt tctgcaattt
     1621 gagagcgtgc ctgctcaact gccatggagg caactctccc atactcatga gttgctgatg
     1681 caggattacc aaaatcccct tcagggccta aatattttat catttgctca acaacgcgag
     1741 gatcaacggg tgttgttgcc atataatcaa aataaatcgg ttttattgcc aattttattg
     1801 ctcctattgt ctttccttgc ccactccaat gatttctgaa cctggctaag catacctctt
     1861 aaaatactaa cttccatttt ttcaaggttg attctattaa acagtcgcct cactctttgc
     1921 attaatctcc ttggattaga gggctttaaa aaattaattt caataaaaac ttctttcaaa
     1981 tgctcataaa attgctctat ttcatcagct gtagccggtt cttcattgcg cagagctact
     2041 tgagcacttg gtgataataa cttcatacga agttcataag cgataatttg tactgcctga
     2101 gccaaattta atgaactgta ctcaggattg cttgggatat tgatatgata gtgacatttt
     2161 aacagctctt cattagtaag gcctgcgtgt tctctaccaa atacaatggc cacttgagta
     2221 ttatctgatt gttgatttat caattctgca caggaagctg gaattaatcc aggtaatgac
     2281 agtccacggg gtctggcact cgtccctaaa atcaactgac atccaattaa agcttcgtcc
     2341 agggtatttg taacaactgc tgcatccaga acatcatcag ctccagcagc catttctttt
     2401 gctttgagat caggaaatga ctttggatta accaggtaca gtgtacttag ccccatggtt
     2461 ttcatagctc ttgcagtaga gcctatattg ccaggatggg aagttgaaac caaaacgatt
     2521 cgaatagaac ttaacttcat aaaaaattat tccaaataca atagataact atatcatagg
     2581 atttattaat tgaacttctc tttgtttaaa aaaaatgtgt tagaattgct gattttatag
     2641 ctaaagagta tatccatgga accactttta aatattgctg tcaatgctgc gcgacaggct
     2701 ggtgaaatca ttaatcgtta tgtagagcaa gttgatcgtt taaaaataac tcccaaaaac
     2761 agccatgaat attttagtga agtggacatt aaagcggagc aagttatcat taatacgata
     2821 cataaagcct atccagagca tggaattctt gctgaagaaa gcggcattca gcaaagcgat
     2881 agtgatacag tctggatcat tgatccattg gatggcacct caaattatct tcatggattc
     2941 cctttttatt ctgtatccat agccctgaaa attaaaaatc gtctggagca tggtgttatt
     3001 tacgatcccc tgcgacatga gtgttttgct gcaagcagag ggcgcggggc aaggctaaac
     3061 gatagaagaa tccgtgtgtc caagcaaact caactcaatg cctctctgtt aggcacaggt
     3121 tttcattttc gagatgcaac tcttgcccag cgatatttac caacctttga agcattgatt
     3181 ggaaaatgcg ctggagttag aagaacaggt tctgctgctc tcgacttggc ttatgttgca
     3241 agcggtcgtc tggatggctt ctgggagttt gggttacgcc cgtgggatat tgctgcggga
     3301 gctctgctgg ttcgagaggc cggaggatta ataagtgatg tccagggtgg agaagatttc
     3361 ctaaattatg gcgacgtagt agcaggaact cctaaagtat ttaagtcact tttgcaaacc
     3421 atatcacccg ttttaaaata agttgtcaat ttaagctcat ttaagacgtt catttaggct
     3481 tatcgatttt aaattatttt cgaatatccg ctctctcgaa gctgagaaag cggataaata
     3541 ctcctctcca ctcttatctt aatcctggct tcattcctaa aattactggc aactcatctg
     3601 ccatcgccgt gcccaaattt ttagttaggc ggtcaaagac attttgcttg taagtatagt
     3661 caactatatc agtaatttta attacctctc gcgccaactg cccgctactg gcataaccat
     3721 caattaaacc attagccaac gcttgctcgc cagtccaaaa taacccggaa aaagtctcat
     3781 catcaacatg taatctattt cctcttcctt ctttgacacg agtaataaat tgcttatgaa
     3841 ctatatcaag catggtttgt agcttttctt tctgaaattc agtcgttggg gaaaaagggt
     3901 ccaggaaagc tttattgact cctgaagttt gtaatctgcg tgaaatacca agcttctgca
     3961 atgcatcaac aaaaccaaag ccattatata aaacacctat tgagccgacc atgcttgcag
     4021 gacttgcata gatttcatct gctccaactg caacataata ggcagcagaa gcacacatat
     4081 cgacacaaac agcgtaagtt ttaatgtttg gttttagact tttataatat tgtaaaacat
     4141 tataaatata ttccgcctgt acaggactgc ctccagggct atttatccgt acaattaatg
     4201 cttttaatcc tttactttta taagccttct caattccttt agtgaaatta tcagcacttg
     4261 ctgaactgga atcagcaatt tcaccgttaa tatcaattag tccaatatgc tctttatcct
     4321 tagatgcttc cgtggttttg gcgactgaaa caatcttata agatgaataa actaatacga
     4381 ccaggataat agctcttata atccatttcc atcgacgctt tcttttcctt tcttggagat
     4441 agtcaattac caattgatta agcaaagctt gtgaatccaa attagaatta gaagatgact
     4501 cattattcat aaaaaactct tgaaaaaatg taaataatac ccattttatg gcatgtaaac
     4561 tagaattaat accctaaaat aactagaaag gtgttcctat gcgaatggac aaactaacat
     4621 caaagtttca aatggctctt gcagatgccc aatccctggc tttaggtaga gacaatggat
     4681 ttatagaacc agaacattta atgaaagcct tgcttgatca gcaaggcgga agttgcagac
     4741 ctcttctcag taaagcgggt gttaatatac cactattaag aactttaatt gaccaagctc
     4801 tggataaact ccctaaagta tcaggcaccg gaggagatat tcatatatct aatgcattga
     4861 atagactact taacctgact gacaaattat cgcaacaaag aaaagataac tttatttcaa
     4921 gcgagctttt tgtattagca gcgataaatg aggatagtaa ccttgcaaaa atactcaaac
     4981 aagccggggg tgacaacaaa gcaattgaaa aagcgattga tgagttaaga ggtggagaaa
     5041 cggttaatga tccaaatgca gaggaacagc gacaagccct tgaaaaatat acactggatc
     5101 tgactgaacg tgccgagcaa ggaaaattgg acccagtgat tgggcgagat gatgaaatca
     5161 gacgcaccat ccaggtattg caaagaagaa ccaaaaataa ccctgtatta ataggtgaac
     5221 ctggtgttgg taaaacagcc atagtagaag gtttggcaca acgaattatt aatggtgaag
     5281 tacctgaagg attaaagaat aaacgcttac tggcactgga catgggtgcc ttgatcgcgg
     5341 gtgcaaaata tcgtggagag tttgaagaac gtttaaaagg tgtgcttaat gatttggcaa
     5401 aacaagaggg tcaaatcata ttatttattg atgaattaca cactatggtt ggcgctggca
     5461 aggctgaagg agctatggat gccgggaata tgctcaagcc agcattggct agaggtgaat
     5521 tgcattgcat tggggctacg acattagacg aataccgaca atacattgaa aaggatgctg
     5581 cccttgaacg acgctttcaa aaagtactgg tcgatgaacc cagtgtggaa gacactatcg
     5641 ctatattaag gggacttaaa gaacgttatg aagttcacca tggagttgaa attacagatc
     5701 ccgctttggt tgctgcagca atgctatccc atcgttacat cagcgaccgt cagctacctg
     5761 ataaagccat tgatttaatt gatgaagccg ccagcttaat ccggatggaa attgattcaa
     5821 aaccagaaag tatggataaa ctggaacgac gtttgattca gctaaaaatc gagcgtgaag
     5881 cattgaaaaa ggaaaatgat gaagcgtcta aaaaaagact tgttgattta caaaaaagta
     5941 tcgatgaatt agagcaaaat tattcagact tagaagaaat ttggaaagct gaaaaagcga
     6001 ccatgcaagg ttccactcaa atcaaagaag cacttgagca agccaaactg gaaatggaaa
     6061 ctgcaaggag agcaggagat ttaagtcgca tgtccgaatt acaatatggg cgtatacccg
     6121 agttggaaaa aagattaagc caggtctctt ccgtcgatgc tatggaaaca aagttggtac
     6181 gcaataaagt caccgaagat gagatagcag aagttgtttc aaaatggaca gggatccccg
     6241 tatctaaaat gatggaagga gaaaaggaaa aactgctgaa aatggaagaa gctttgcaca
     6301 gcagactcat tggccagaat gaagcggtgg atgctgtttc taatgccata agacggtcac
     6361 gcgcaggcct gtctgatcct aaccgtccaa ttggttcatt tctttttctg gggccaactg
     6421 gtgtgggaaa aactgaacta tgtaaagcac ttgcttcttt tctttttgat acggaagagg
     6481 cgatggtgcg tattgatatg tctgaattta tggaaaaaca ctctgttgcg cgcttaatcg
     6541 gagcgcctcc tggctatgta ggatatgagg agggtggtta tttaactgaa gcagtcagac
     6601 gcaggcccta ttctgttatc ttacttgatg aagtagaaaa agctcataca gatgtattta
     6661 atattctgct tcaagtgatg gatgatggcc gtctaacaga tggtcaggga cgtactgttg
     6721 actttaggaa taccgttatt gttatgacct ctaatctggg atctcaactc attcaggaaa
     6781 tgagcagcaa atttaattac gatgagatta aggctgcggt aatggattta gtcagccaac
     6841 atttcagacc tgaatttatc aacagaattg atgaatcggt ggtattccat tccctgacaa
     6901 aagaacaaat tgcaaaaatt gcagcaatcc agattaacta tttacatcat cggctgaaac
     6961 aacaaaacat cactcttgaa gtcacaagtg aggctttatc tcatttggct gaagcaggat
     7021 ttgatccggt ctatggtgcc cgccccctaa aacgtactat tcagcaaaaa ttagagaatc
     7081 cactagctca atcattattg actggaaaat ttaaatccgg ggataccatt attgtatctt
     7141 ataaagatgg agtaatggaa ttttccaagc aatgagtatg cttatcagtc gaaagtaatg
     7201 caggagtcct tatccaggac tccaaaatta cggggtaatg tttcaagacc aagctgggta
     7261 aacatatcaa acaaataaag acgtagtgcg agtctctgaa ttatcttcct gggctttgtt
     7321 tactgtttga actttttttg ctaaagccaa ttctgtttga agaataaatg tattcattga
     7381 gccttttcca ttaaaaaaat caaaattaag ctcaacttta ggattagctt caaaatatgt
     7441 tttaagaagc ctgattgcat caagcatatc cttatcagtc aactcattgg gatttatttc
     7501 cacacccaga gcctggtgaa tgcaaaaccg taattgctcc ttaacgccct cgttttgtaa
     7561 taacaaacag taaccgccca aagcccgata tcttgcaaga ctgttattac aatttttcat
     7621 tgcttcaata aacgaaccac aatccatcgt gctaaaattt tcctgaatat gtccggtata
     7681 caacagatca ataatttttt cctgtaaaac gttcgattca atttgagctt ttatttgctc
     7741 ttccatcagt tccaaggata tggttttaaa atcggaacag gtttttggca agagtttgcc
     7801 taaatgagtc aaagcctctt caatttgacg ttgctcttct tcaatttgtt ttaccaagga
     7861 ttgaatgaaa tttatggcct tgaattgatt taaaacaaca gggttgcgtc ttttgtgttc
     7921 atcaataatt tcttgtaaat aggtttcaaa gttcttgtct gctgcaaaat gaggatagct
     7981 tttatagcgc ggcattttcc cctcatcctt ccctacaatc aattgcatat tatcacgaaa
     8041 tgtttccaaa gcagttacga tcgttgttac atcaagaatt ttcaagtcat ttattctata
     8101 atttttctcc atcacctcag gtaaacctaa agcgagtctt attgcttgaa acaatcgaca
     8161 atcttttaca tcagatggcg ctttatattt aaaaaaaaat gatgggacag gaatataaga
     8221 attaaagtta tcatactcct taatcagtct aaaatagcga tgaagcaaag cacctagcaa
     8281 aaacaggatt gcttgcttat ttttttcctc attattttct tttaaaactt ctcgcaattc
     8341 attacttaat tgcttaaaaa catctctggc agtttctcta tctgttttgc aaatcacaac
     8401 cttatgggtc cgagctaata atgtttccaa agtatttcta attatttcac cactcgatac
     8461 cgcttcagat acaggaggat cagtaataat tttatacctg cctagcaagg tagagaatat
     8521 tgtctctaag ttacgagtat ttacacttaa tattttgaac tcgggcggag taaaagtcat
     8581 agtgtctcct gtctaaaaca cgctattaag tcctaaagta cgcataattc ctttataaat
     8641 atcatagaat gaagtttcct gtttctccct ggaatgacgt aaggcatgaa tatctgcttg
     8701 caccgtctta cctaaacggc gaattaagtt gtcataaatg cgttcattac ggttttgata
     8761 ggcaaatgaa caatattgac ttaaagcctc taaactggta tcaaacagag tgtcactcat
     8821 cttttgcggg gagctcactt gcaaatctct ttgaaatagc tcaaccaaag tgctattcat
     8881 taaattcgaa acagtagagc tataatgtga ccacatcatt aaataaattc cagttagtac
     8941 cttttttttg aattcgttaa tattttttgg agttacagca gagtatcttg tgacaccctt
     9001 tagataagcg attaagtcat cgtcattctc gctatccagg ctataagcaa ccaatctaat
     9061 cgtatgagta tcttcaatgg ccgctgtatt gggtaatatc actgaaaaaa aacaaaaaca
     9121 gcctaaggat ggtatgctgc gtttctttaa ttgatctgtt aatttcaatg cattttgctg
     9181 aaattcggca aactctagca tggtttccct cccacaaggg cccacccgca agaaccgcag
     9241 cgagtaatac gcctttgatg atcaagactc ttttaatttt tatatcaacc tacacataat
     9301 attgc
//
LOCUS       DBNCWK010000120         9245 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.120, whole genome shotgun sequence.
ACCESSION   DBNCWK010000120 DBNCWK010000000
VERSION     DBNCWK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.120"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(205..696)
                     /locus_tag="AC06IO_002348"
     CDS             complement(205..696)
                     /locus_tag="AC06IO_002348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875522.1"
                     /translation="MIIKDMSIIGGIPGAITALAAIITKNSVPDSITAGVISSLSACS
                     SVEAVRYAFLYRTCPPIADVALFTGGAILSGQILLTMAQLLITTDLSRPALALNQAVV
                     VSSLLLGMMIYRDLKYRVNLINGNSGTSFFGSIKQTFFGSNSTPPKNENENHPNAQRS
                     LAI"
     gene            1138..1635
                     /locus_tag="AC06IO_002349"
     CDS             1138..1635
                     /locus_tag="AC06IO_002349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875523.1"
                     /translation="MRGLDPAMVLTYRYEYSEYLHENYTSKILLIQEWLSQILSSEKV
                     NELPIKANEKLNQYLLLSHCTEQANCSEALIQWQKIFQFFGLTLVAEKTGCCGMAGAY
                     GNESIHQFNSHGLFDLSWKDYFECSTGLSNTLVDGYSSCRAQVKRLTGLNASHPAEIL
                     LKLFE"
     gene            1653..2087
                     /locus_tag="AC06IO_002350"
     CDS             1653..2087
                     /locus_tag="AC06IO_002350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875524.1"
                     /translation="MSPPNIDLIILSYGLILRYGVTVINSQSNGVFTELKNIEGRLGD
                     VCYGKRGQEIVPIFADVIRQHMISSGVSFEDYLICQQTLTQFTIQISPEISNKNQFIE
                     HLNQLFIYKNCELPQWNWELYDKSQPGVKRRRVRSLYCPKTE"
     gene            complement(2181..3041)
                     /locus_tag="AC06IO_002351"
     CDS             complement(2181..3041)
                     /locus_tag="AC06IO_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875525.1"
                     /translation="MKKILITLIGFLFMNTYHHLLYADNSHHHTAKDLSASSSKLENT
                     PTIQLEVQKIIDKKDKKLVFVKLTDIKSNKPITFNDLTEIHTKKFHLLIIDNHLIDYS
                     HVHPAETATPGIYQFEWQPNFKNATYRAWADLLPTNTNKQEYATADLTSPQTVRRKMN
                     PIHHRTVLESKVNGYQFKLTFNKMPLLAGKPAMGEIDVTDSKGHPVRTLEPIMGAYAH
                     IVGFNEDFKTVIHVHPMGKEPENSTERGGPQLQFHIEPNKAGFIKMFAQVQIDGKVLY
                     APFAFTVKNA"
     gene            3249..5453
                     /locus_tag="AC06IO_002352"
     CDS             3249..5453
                     /locus_tag="AC06IO_002352"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-transporting P-type ATPase"
                     /protein_id="HIO9875526.1"
                     /translation="MAHNHHEGHSHVHNEHACCHKKGHAHKPQISIEGATFYTCPMHP
                     EVRQAIPGICPLCGMALEPEAISIEEATNPEYLDMKHRFWLALILTLPVVVLEMTGHG
                     FQHFISTDISNWIQLLFATPVVLWSGWPFFQRGWQSLKTRQLNMFTLIAMGIGVAWIY
                     SLVAILFPGLFPAAFRSQEGVVAVYFEAAAVITTLVLLGQVLELKAREQTGSAIRALL
                     KLAPENAHRIKEDGSEEEISLDQVVVGDLLRVRPGEKIPVDGEIQEGNSFIDESMVTG
                     EPMPVAKEIGDKVIGATMNQTGSFIMKALHVGSDTMLARIVQMVSDAQRSRAPIQRLA
                     DTVSGWFVPAVILIAVLSFIVWALFGPQPAFGYGLIAAVSVLIIACPCALGLATPMSI
                     MVGVGKGAQNGVLIKNAEALERMEKVNTLIVDKTGTLTEGHPKLTHIETSEDLDESQA
                     LLLAASLEYYSEHPLANAIVKAAKEKQMSFSSVHNFEAPTGMGVVGKVNGHQVAIGNA
                     KLMQEYGSDNESLFEKADKFRAKGSSVMFMAVDGKTVALLVVEDPIKSTTAETIHTLQ
                     KKGIEIYMLTGDSKKTAEAVAATLGIKNVIAEIMPEDKGRIVSELKENGFVAMAGDGV
                     NDAPALAKADVGIAMGTGTDVAIESAGVTLLHGDLRGIAKARYLSENTMNNIRQNLFF
                     AFIYNMLGVPLAAGIFYPLTGLLLNPVIAAAAMALSSVSVIVNALRLRRIKL"
     gene            5698..6093
                     /locus_tag="AC06IO_002353"
     CDS             5698..6093
                     /locus_tag="AC06IO_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875527.1"
                     /translation="MQNEKEVKELIQSDKKKRVTWNDNQTKGQIDEKFLRIGTGSTPY
                     KNKLDEEIAQLIKEGYSEIEATSIVGKPLTPSGTLMLNNPIFYKSKESIDTEKANPYS
                     FFSIRNIGLGVVAIAAAAATVGLVVSKNS"
     gene            complement(6118..7101)
                     /locus_tag="AC06IO_002354"
     CDS             complement(6118..7101)
                     /locus_tag="AC06IO_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIO9875528.1"
                     /translation="MIVNSSFRPVWWLSNRHAQTLYPTLVKKRLKPPIDFHERLELPD
                     GDFIDLAWSINDLNKNTPLVILLHGLGGGINSIYVSGLMQAFANAGFRCVLMHFRGAS
                     EEPNRILRTYHSGDTADFAYFLEILAKREPATKKAVVGISLGGNVLLKWLGETASSLW
                     VDAAVAVSVPFQLNSVADRMNQGFSRLYQAYLLSRLRRIFLKKLNHLNNSPFSKNDLL
                     SLKTFWDFDERITAPINGFKNAHEYYQKASSRQYLKNITTPTLIIHAQDDPFMTPDAI
                     PTSKELSSQTTLELSKYGGHVGFIAGTGKSSSQPVYWLEQRIPEFLLDYLS"
     gene            7341..8357
                     /gene="truD"
                     /locus_tag="AC06IO_002355"
     CDS             7341..8357
                     /gene="truD"
                     /locus_tag="AC06IO_002355"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HIO9875529.1"
                     /translation="MYSLNWPRAYGMPNSTATFKLCPEDFQVNELFEGQFSGEGEHIV
                     LKIEKKGLTTEQVVKSLAGLINKPIKLISYAGLKDKQALTTQWLSIHAPGEVIEGIET
                     LEAPGWKILECTRHNKKLRPGFLSGNHFTITLRNVSNETDLIHRIEQIKLKGVPNYFG
                     EQRFGRDGGNLIKAEEILVQGRKVKDRFLKGMYFSAARSWLYNLILSRRVKESSWNLP
                     LLGDVIQLVGSNSVFVNDKSLDEQLLQRIGEKDVSPASPLPGRSKNLVKGTALQIINE
                     VYAEWSAWLDGLEKNGLEEAWRANILYAEQIEYRINQGTVELSFVLPAGAYATVVLRE
                     LVQY"
     gene            8489..>9245
                     /locus_tag="AC06IO_002356"
     CDS             8489..>9245
                     /locus_tag="AC06IO_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875530.1"
                     /translation="MNLVQWMPPFFWLVILAIYSTPSMALSVELIETSPKSPVTLNQD
                     EALYVLIRYKSDQPLRFQAIGENLQQKIMDSARFNPSQAYPAGEGEAIAWVAYDNTTE
                     IDSITVTIYDANWRALQTKSIPVSAVWQNENGRNNQVAAPWVQRLNQQQQSSVFTNSQ
                     TSLSSGDAHFVQFLFLLIPLYWLVQIFILFNWTGRWKKLACFPLLFSVPLLLYTFYAL
                     YAGSNLWPLMMLFVTPFILALLLIIIGYKKVYSR"
BASE COUNT         2856 a         1618 c         1923 g         2848 t
ORIGIN      
        1 gttttatttc tcttatatac aacaaatatt cattttgcat agcttccata ctttcatcta
       61 ttagttaaaa aatgcggcaa tcatagcatg atataggggc tataaaaaat ttttgagctc
      121 gataagattg actcgcaatt tattatttaa gagcgacact catcgctctt aaatatgctc
      181 tacaaatcat gtaaaggatg ttgtttagat ggctagactt ctctgagcat tggggtggtt
      241 ttcattttca tttttgggag gtgtcgagtt agacccaaaa aatgtctgtt taattgagcc
      301 gaaaaaagat gtacctgagt tgccatttat aaggtttacc ctgtatttta aatcacgata
      361 gatcatcatg ccaagaagga gtgaggagac aactactgct tggttgaggg ctaatgcagg
      421 tcttgataag tctgtggtaa taagaagttg ggccattgta agtagaattt gtccagatag
      481 gatagccccc cctgtaaata gagccacgtc agcaataggc gggcaagttc gatagaggaa
      541 tgcatatcta acagcctcaa ctgaactaca tgcactaagt gatgaaatga cccctgcagt
      601 tatgctatct gggacactgt ttttcgttat aatggcagct aaagcagtaa ttgctcctgg
      661 tattccacca attatactca tatcttttat aatcatgttt gtatcctctg ttaaagtaaa
      721 aggcggataa taggaataag gagcacaatg tcaaccacac atccagtagg ccatgcaaat
      781 tctacttgca tagcttccat atcttcatcc attagttaaa aaattggtac cttttctgca
      841 aggcagaata atacctcaat agtcaatctc cgctcaatct cccatcttat tttgaatagc
      901 atattgatta tccgctagcc ctgccatcaa ggccatttct gctgataaat tgcggtttaa
      961 agtaaaattt ttgcaaaccg atcaccgtgc tattctcgct caataaaaga ttatcttatt
     1021 gcttacagcg aacgtgttgg atattttcaa agcctgtttc cagaattagc taatttctgt
     1081 ctctcaaata aattagtgaa atattgttta cataagctat cagatcatgg attaaccatg
     1141 agagggcttg atccagctat ggtattaacc tatcgatatg aatattctga atatttacac
     1201 gaaaattaca ctagtaaaat cctgttgatt caagagtggc tctcacaaat actcagtagt
     1261 gaaaaagtca acgagttacc aataaaagct aatgaaaaac ttaatcagta tttattgcta
     1321 agtcattgca cagaacaggc aaattgcagt gaagctttaa tacagtggca gaaaatcttt
     1381 caattttttg gtttgactct agttgctgaa aaaacagggt gttgtggtat ggctggagct
     1441 tatggaaatg agtcaatcca ccaatttaat tcccatggct tgtttgattt atcatggaag
     1501 gattattttg aatgcagtac tggtttatct aatactttag tcgatggcta ttcatcatgt
     1561 cgagcacaag tcaaacgatt aaccgggtta aatgcttctc atcctgctga aatattactg
     1621 aaattatttg aatagatata agctggaatt gattgtcacc gccaaatatt gacttaatta
     1681 ttttatcata tggactaatc ttgcgatatg gtgtcactgt tatcaattcg caatcgaatg
     1741 gtgtatttac tgagttaaag aatattgaag gccgtttagg tgatgtttgt tatgggaaaa
     1801 gagggcaaga aatagttccc atttttgcag atgttattcg acagcatatg attagcagtg
     1861 gtgtaagttt tgaagattat ttaatttgcc agcagacatt aactcaattt acaattcaga
     1921 tatcacccga aataagcaat aaaaatcaat tcattgaaca tttgaatcaa ctttttatct
     1981 ataaaaattg cgaattaccc caatggaact gggagctcta cgataaaagc caacctggtg
     2041 tgaaacgcag aagggtccgc tcactatact gccctaaaac agaatgagcg ggtacaaaga
     2101 actggtttgc tctgcacaat acaagccatg agcttacaat aatatttgcg tctttaaaag
     2161 acagtctctt aatttagtcg ttaagcattt tttactgtga atgcaaatgg cgcgtagagc
     2221 actttcccat caatttgcac ctgggcaaac attttaatga aacctgcttt attaggctca
     2281 atatggaact gtaattgagg tcctccacgt tcagtgctat tttcaggctc tttgcccatg
     2341 gggtggacat ggataacggt tttaaaatct tcattaaagc cgacaatgtg tgcataggcc
     2401 cccataattg gttctagtgt acggactgga tgccctttag aatcagtcac atcgatttca
     2461 cccatcgcag gtttacctgc tagtagaggc attttattga aagttaattt gaattgatat
     2521 ccattcactt tactctctaa tactgttcga tgatgtatag gattcatttt tcttcttact
     2581 gtctggggag atgttagatc agcagtggca tactcctgtt tattggtatt ggttggtaat
     2641 agatcagccc atgcccgata ggtggcattc ttgaaattgg gttgccattc aaattgataa
     2701 atccccggag tagcagtttc tgcgggatgg acatgactat agtcaatgag atgattatca
     2761 atgatcagta aatgaaattt ttttgtatgt atctcagtaa gatcattgaa agtaattggt
     2821 ttattcgatt ttatatcagt gagtttgaca aataccagtt tcttatcctt tttatcaatt
     2881 atcttctgga cttccaattg tattgtgggt gtattctcca atttagagga agatgctgaa
     2941 agatctttcg ccgtgtgatg gtgggaatta tcggcataga gcaaatgatg ataggtgttc
     3001 atgaataaaa agccaattag cgtgatgaga atttttttca caatacaatg atctccgtaa
     3061 tttttattgc caagctaaat ttttcttctg tcaacgatga attataattg aaagtagctg
     3121 attcttacag ggtaaaaaat gcattattta ttatgatctt gtagatataa accagtacaa
     3181 cagaatatca tcagctaatt taaatggtta taaattccag gcaaaataaa actacggata
     3241 ggtgaataat ggcacacaac caccatgaag gtcattccca tgttcataat gagcacgctt
     3301 gttgccataa gaaaggtcat gcacacaaac ctcaaattag catagaggga gcaaccttct
     3361 atacttgtcc tatgcatccc gaagttcgac aggctattcc ggggatttgt ccactttgtg
     3421 gaatggcgtt agaaccagaa gcaatttcca ttgaagaagc aacaaatccg gaatatctcg
     3481 atatgaaaca ccgattttgg ttggcgttga tattaacttt acctgttgta gtcttggaaa
     3541 tgactggaca tggattccag cattttattt cgacagatat ttcaaattgg attcagttac
     3601 tcttcgcaac acccgtagtg ctatggagcg gttggccttt tttccaacgt ggttggcagt
     3661 cgctcaaaac acgccaatta aatatgttta ctttgatagc gatgggtatt ggtgtagcgt
     3721 ggatatatag tctggtagcg atcctttttc caggattatt ccctgccgcg tttcgaagtc
     3781 aggaaggagt ggttgctgta tattttgaag cagccgctgt gataactact cttgtattgc
     3841 tagggcaagt gcttgaatta aaggcacgag agcaaacggg tagtgccatt cgtgctttgc
     3901 tgaaattggc accagaaaat gcgcatcgta ttaaggaaga cggtagtgag gaggaaatat
     3961 cactcgatca ggtagtagtt ggtgatttgc tgcgtgtacg gccaggtgaa aaaatacctg
     4021 tggatggtga aatacaagaa gggaatagtt ttatagatga gtctatggta acaggtgaac
     4081 ctatgccggt tgctaaagaa attggagata aggtgattgg tgccacaatg aatcaaacag
     4141 gcagcttcat tatgaaagca ttgcatgttg gatctgatac catgctggca cgtattgttc
     4201 aaatggtgag tgacgcccag cgaagcagag ctcctattca gcgacttgcg gatactgtct
     4261 caggatggtt tgtgcccgca gtgatcttaa ttgcagtatt atctttcatc gtatgggcgc
     4321 tatttggtcc acagccagct tttggatatg gattaattgc agctgtatca gttctcatta
     4381 ttgcttgccc atgtgcactg ggtttggcaa caccaatgtc tatcatggta ggtgtgggaa
     4441 aaggtgcaca aaatggagta ttaatcaaaa atgccgaagc acttgagcgc atggaaaagg
     4501 tgaatacgct tattgtagat aaaacgggta ctcttacgga aggacacccg aaacttaccc
     4561 acattgaaac cagcgaagat ttggatgaga gtcaagcatt gctattggct gcctctctgg
     4621 aatattacag tgaacatcct ctggctaatg ccattgtcaa agccgcaaaa gaaaagcaaa
     4681 tgtctttcag ttcagttcat aactttgaag cacctaccgg gatgggggta gtaggtaaag
     4741 ttaatggcca tcaggtagcc atcggtaatg caaaattaat gcaagagtat ggaagcgaca
     4801 atgaatctct ttttgaaaaa gctgataagt ttcgtgcaaa aggttcgtct gtgatgttta
     4861 tggcagtcga cggtaaaaca gtagcgcttt tagtggttga agatccaatt aaatcaacta
     4921 ccgcagaaac cattcataca cttcaaaaaa agggtattga gatttacatg ctgactgggg
     4981 atagcaaaaa aaccgcagaa gccgtggcag ctactctggg tataaaaaat gtcatagcag
     5041 aaataatgcc ggaagataag ggtcgtattg tcagcgaatt aaaagaaaat ggttttgtcg
     5101 ccatggctgg tgatggagta aatgatgctc ctgcactggc aaaagcagat gttggtattg
     5161 ctatgggtac agggactgat gttgctattg agagcgctgg cgttaccttg ctacatggag
     5221 atttgcgtgg tattgccaag gcccgatatt tatcagaaaa cacaatgaat aacatacgtc
     5281 aaaatctttt cttcgctttt atttataata tgttgggtgt tccgctagca gcaggtattt
     5341 tctacccact aacaggtctg cttttaaatc cggtcattgc tgcggcagct atggcacttt
     5401 cttctgtgtc agtcatcgtt aatgccctaa gactgcgacg aatcaaactg taaatcggta
     5461 agtataacac tttggtaaag tgttttaaat tttctgtaat gacttctggt tatgctagtc
     5521 agaaataaat tgataataaa cagaaagtta cactgcgata tagaattcca tcaatcgact
     5581 cgatttcacc tggggtttta attacccgaa atttttaaat tatcttttga gttcatattt
     5641 tcttaagtat ttattgctaa aattgttcaa attttgttta ttaatagaga ggtttgcatg
     5701 caaaatgaga aagaagtaaa agaactcatt caatcggata agaaaaaaag agtcacatgg
     5761 aatgataatc agactaaagg gcagattgac gaaaaatttc ttagaattgg cactggttca
     5821 actccgtata aaaacaaatt ggatgaggag attgcccagc ttattaaaga aggttattca
     5881 gaaatcgaag ctacatcaat tgttggcaaa cctctaactc caagtggtac gcttatgttg
     5941 aataatccaa ttttttataa atcaaaagaa agcatagata cagaaaaagc gaatccttat
     6001 agctttttct caataagaaa tattggatta ggtgtagtgg ctatagcagc agctgcagca
     6061 acagtcggac tagttgtatc aaaaaacagc taaacgtatt gcattcccat taatttttta
     6121 agataaataa tctaacaaaa actcagggat tcgctgttca agccaataca caggttggct
     6181 tgagcttttt cctgtaccag caataaaacc tacatgtcca ccatacttac ttaattccaa
     6241 agtggtttga gacgatagtt ctttcgatgt tgggatggca tctggtgtca tgaacggatc
     6301 atcttgggca tgaataatca aagttggcgt ggtgatattc ttaaggtact gtctggagct
     6361 tgctttttgg tagtattcgt gggcattttt aaatccgttg atcggtgctg taattcgctc
     6421 atcaaaatcc cagaaggttt ttaaggaaag taagtcattt tttgaaaatg gcgaattatt
     6481 taagtgattt aattttttta gaaaaatacg gcgcagtcga cttaataaat aagcttgata
     6541 caggcgagaa aaaccctgat tcattctgtc agccaccgaa tttaattgga atggtacgga
     6601 aacagcaaca gctgcatcta cccataaaga tgatgcagtt tcacccaacc atttcagcag
     6661 aacattgccc cccaacgata tgccgacgac cgcttttttt gtagcaggtt ctctctttgc
     6721 caaaatttca agaaaataag caaaatccgc cgtatcaccg gaatgatagg tacgcaaaat
     6781 ccgattgggt tcttcactgg ctccgcgaaa atgcattaaa acgcaacgaa accccgcatt
     6841 ggcaaatgct tgcatcaatc cactgacata aatggagtta atacctccac ccaaaccatg
     6901 gagtaatatg accaaaggtg tatttttatt taaatcattg atagaccagg ccagatcaat
     6961 aaaatcaccg tcaggcaatt ccaggcgctc atgaaaatca attggtggtt ttagccgctt
     7021 ttttactaaa gtaggatata aagtttgtgc atgcctgtta cttagccacc acacgggtct
     7081 aaatgaacta tttacaatca tactgatagc ctgaaaataa aaaatcgaat tctaaccgga
     7141 taaagtagac caagattaga gctagcaaaa aaatacttac ttaaattata gtcaaccaat
     7201 cattgttggt aatcttgcat cataatgttt tatagtcgcc atatcaaacg atgttaagat
     7261 aaattatgta tcagctactc ttgatatcat agtgaatttg tgtatttagt ttagaaccct
     7321 ttttgttgaa ggagatcttg atgtactctt taaattggcc acgtgcctac ggcatgccaa
     7381 actctacagc tacttttaag ttatgtccgg aagattttca agtgaatgaa ttgtttgaag
     7441 gacagtttag cggtgaagga gaacacatag ttcttaaaat tgaaaaaaaa ggactaacca
     7501 ctgaacaagt tgtcaaatca ttggctggtc taattaacaa accgataaaa cttataagtt
     7561 acgcgggatt aaaagacaaa caggcattaa caacgcaatg gctcagtatt catgcacctg
     7621 gagaggtaat tgagggtata gaaacactag aagcaccagg atggaaaata cttgaatgca
     7681 cgcgtcataa taaaaaactg aggccaggtt ttttatcagg aaatcacttc acaatcacat
     7741 taagaaatgt ttctaatgag acagatttaa tacaccgtat tgaacaaatt aaattaaaag
     7801 gtgttccaaa ttattttggt gagcagcgtt ttggtcgaga tggtggaaat ttaattaaag
     7861 cagaggaaat actagtacag gggcgcaaag taaaggatag atttttaaaa ggaatgtatt
     7921 tttccgcagc ccgatcatgg ctatataatt tgatcctgtc tcgccgtgta aaggaatcat
     7981 catggaattt gcccttattg ggggatgtca tacagcttgt tggatcaaac agtgtatttg
     8041 ttaatgataa aagcctggat gagcaattgt tgcaacgaat cggtgagaaa gatgtttctc
     8101 cagcaagccc tctgcctggc aggagtaaga atcttgtaaa agggacagca ttgcaaataa
     8161 taaatgaggt ttatgcggaa tggtctgcct ggttggatgg attggaaaaa aatggtttgg
     8221 aagaagcctg gcgtgcaaat atcctttatg cagaacagat tgaatatagg ataaaccaag
     8281 gtacagttga gctttcgttt gtcttgccgg ctggtgctta cgcgacagtg gtattacgtg
     8341 aattggttca atattaagct ttgtttgcac ttgaatagca ctttagtcaa tgtggagtaa
     8401 ttattttaat tgaattgatg agttaatcac tatgtttgat gttatgccat aataaagaga
     8461 aatgtttatt taattgaagg acacattcat gaatctggtt caatggatgc cccctttttt
     8521 ttggctagtg atcctggcta tttattcaac tccatccatg gcattgagtg tggagcttat
     8581 cgaaaccagt cctaaatctc ctgttacatt aaaccaggat gaggctttgt atgtgcttat
     8641 tcgatataag agtgatcaac ctttgcgatt ccaggctatt ggggaaaatc tgcagcaaaa
     8701 aataatggat agtgctaggt ttaacccatc acaagcgtac ccagccggag aaggtgaggc
     8761 tattgcctgg gtagcttacg ataataccac agaaattgat tccattactg tcacaatcta
     8821 tgatgccaat tggagggctt tgcaaaccaa atcaattcct gtgtctgcag tctggcaaaa
     8881 tgagaatggc agaaacaatc aagtagcagc tccctgggtt caaagactga atcagcaaca
     8941 gcagtccagt gtatttacta actctcagac ttctctttct tcgggggatg cccattttgt
     9001 tcaattcctt tttttattaa tccctttgta ttggcttgtg caaattttta ttctttttaa
     9061 ttggacgggg agatggaaaa aattggcgtg tttcccttta ttatttagcg tacctttgct
     9121 gttgtatacg ttttatgcgt tatatgcggg ttctaattta tggcctttga tgatgttatt
     9181 tgtgacacca tttatcctgg cacttttatt gatcattatc gggtataaaa aggtctattc
     9241 cagat
//
LOCUS       DBNCWK010000121         9147 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.121, whole genome shotgun sequence.
ACCESSION   DBNCWK010000121 DBNCWK010000000
VERSION     DBNCWK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.121"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(28..117)
                     /locus_tag="AC06IO_002357"
                     /pseudo
     CDS             complement(28..117)
                     /locus_tag="AC06IO_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(179..472)
                     /locus_tag="AC06IO_002358"
     CDS             complement(179..472)
                     /locus_tag="AC06IO_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HIO9875531.1"
                     /translation="MMSKFFNLKTGTLHSVESYAGAVKLPKDISQESIRINERILDND
                     GYPLMAPKLRRQNANQLRMFFSRESNTLLNGSYDLADRSNKDLTEVANIISYR"
     gene            561..941
                     /locus_tag="AC06IO_002359"
     CDS             561..941
                     /locus_tag="AC06IO_002359"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIO9875532.1"
                     /translation="MNKTYTEKLFSYGTLRYEKVQIANFGRKMQGQEDVLQGFKLTTI
                     EIKNSAVVATSGNNFHPIISYTGNPSDKVPGIVFTISKEELAQADKYEVSEYKRVNVK
                     LNSGVNAWVYINAQDLYGNNQTKN"
     gene            complement(1217..1837)
                     /locus_tag="AC06IO_002360"
     CDS             complement(1217..1837)
                     /locus_tag="AC06IO_002360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIO9875533.1"
                     /translation="MVEKNKDKVYQVYDEIVDWFDDNRTKDLTMEKLYLDFIQKCLKP
                     KSKILDVGCGTGEPIAQFLINEGYDVTGIDASRKMISLCKQRFPKNKWILADMRTLDL
                     NEKFHAVIAWHSFFHLPHDDQRMTLKLLASYVDQNGLLIFTSGPEYDEVWSNNGGHDL
                     YHASLSTEEYEQILIDNNFKVLAHKIRDPDCGDATVWVAQKASRTY"
     gene            1951..2943
                     /gene="aph(9)-Ia"
                     /locus_tag="AC06IO_002361"
     CDS             1951..2943
                     /gene="aph(9)-Ia"
                     /locus_tag="AC06IO_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HIO9875534.1"
                     /translation="MLRNNITDQHLIALLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     ESNSYFVKLKYGHHDEINLSIIRLLHDSGIKEIIFPIYTRDTKLFQQIDHFKIIVYPF
                     INAPNGFTQNLTEKQWHQLGKVLRQIHETSVPTAIQQRLRKETYSPKWREMVRSFYNK
                     IGFDDSDDQITTDFKTFFNQKIDSIHRLVDSSEELSKKIQLDLDKYVLCHSDVHAGNV
                     LVVNEGSIFIIDWDEPMLAPKERDLMFIGGGIGNVWNKPHEIDYFYEGYGKTNVDKII
                     LSYYRHERIVEDIAVYGQDLLSRDQNNESRLESFKHFKSMFDPNDVVEIAFSSD"
     gene            complement(2946..3359)
                     /locus_tag="AC06IO_002362"
     CDS             complement(2946..3359)
                     /locus_tag="AC06IO_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecDF P1 head subdomain-containing protein"
                     /protein_id="HIO9875535.1"
                     /translation="MIKVIAALVGVFFLPSAMASSPTTTDIDASKDISSLVFQVIQNQ
                     MVLDSSTVESATIVLPETPTDGYGVRIKLKTIAATKFGQMTENGIGKQGSFILNGIVI
                     SNPIIRSRIGDEFIVTGFTKKQANHFIESMSSKNN"
     gene            complement(3432..3728)
                     /locus_tag="AC06IO_002363"
     CDS             complement(3432..3728)
                     /locus_tag="AC06IO_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIO9875536.1"
                     /translation="MTKNKFKATHPLLSLMNVGEATYKDLQLLGINSIHQLANASADQ
                     LYARLQQITGQLHDPCVWDVFAAAINEARTGEKQPWWQWTKIRKKRQLEGTFCI"
     gene            complement(3829..5124)
                     /locus_tag="AC06IO_002364"
     CDS             complement(3829..5124)
                     /locus_tag="AC06IO_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIO9875537.1"
                     /translation="MCALYYNLNNHYASHFVYSPEKIMKKPLDNTEQNRIAALYELKI
                     LDTPADERFDRIIRLTSGYLSVPIAFISFIDSKHQWFKSSCGIGLNKISRNDSFCHYT
                     IQQNKPMIISDALLDERFCNNPYVTNSPNIRFYAGFPLSTVDGFNVGTLCAIDKKPRN
                     LDETEIQILKDLATLAEDQVNLLDISLLERAVREKNISLTKAKKELEMRNNFIRKVFG
                     SFMSDEIVAALLESKSELKLGGEERKITILFSDLRNFTTLSEHFPADKIVAALNNHYG
                     KMVDVIEKYNGTVDSFIGDAIMVVFGAPHSGGDDAYRAIACALEMQATMQEVNTMNRN
                     SSLPELAMGIGINTGYAIVGNIGSQKRMQYSAIGSPVNLASRIQDLTLGGQILISEAT
                     YQEVNRSLELNGHLRVKVKGVAAPITIYDVIGLNKKSDA"
     gene            complement(5290..6318)
                     /locus_tag="AC06IO_002365"
     CDS             complement(5290..6318)
                     /locus_tag="AC06IO_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875538.1"
                     /translation="MRRSKAMQIPKKRTQILTISESSKEDLDVVPEYSEEMDVSPSKE
                     TSKTNSKKLESIARKEQIKKLEGELYSHIQDAIELEEESFAEFSINTGRPSNYLFRFV
                     SEKEYLAILENLNQGKRIFGNKKKVLEEEDEKISTKAKGETFFAFDLKYAQKYFNKGT
                     RRNGVMLAIELHEDADSKLLSNTEIVRRIGDDPGMAGGRWAKCKKVKPGERGVVVAKC
                     EGVRAGCKYPYTLGFREGEEGVSPFDTLFELIRSVKVLDNNGSCKTIYHCSVPAPKEK
                     ELTNIVKDAESGTDGIHSSEFLSVVSSSPSSRFGFFTNNELSGISGLVNKAPEHIIPP
                     SDNTFSHH"
     gene            complement(6528..9077)
                     /gene="lgt3"
                     /locus_tag="AC06IO_002366"
                     /gene_synonym="legC5"
     CDS             complement(6528..9077)
                     /gene="lgt3"
                     /locus_tag="AC06IO_002366"
                     /gene_synonym="legC5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector
                     Lgt3/LegC5"
                     /protein_id="HIO9875539.1"
                     /translation="MKEQQKAIFIDNIKSKCSVDEHMAKELADSLLEIHQLNTGINEQ
                     TLVSEVFLTKFALYLKIGKEIALDWVRKNKDLDTPFPSNAVESQPVQDAKKSVENKVQ
                     VYGAMTVGRSPVNKALNQLTNDLLKEVVEHGKTQKAQKLRAYIFDQLARRLEAALSQE
                     QINDLYNRIRGTGDYTKSESFSEEQLKILKEKVVPELKRELSDLRNGNVNILGLDVSR
                     EDKYAFDTTNIFSVWFSNNPAVYMPQHVKTQVEKTAKLNQPGKTRIVFSSLCLNETAQ
                     IDFQQWAKENNIELVDIDSIDLKSVSETDAQLLNLAKVELEAMRKGKGGNPAAASDLV
                     RWVDVIIGESSTYIDIDLPMNDKKVTVEVHSGFPVLLNMGSALTKDGQQPAMENPAFN
                     TDMIAYSKDKEARRQIIEGVAKKIIARYENCAKYIEESKNEELVRLKNSPGYKLFVEK
                     TDGKFDLCALRAAVSEAHQDALSFATFFGAEYFAKTFATQELIPVIKEAIQHQNQELL
                     TSVIENHIEKQHLNDYPKTPDGIKKLLKSFQGIVYKPLVMEFSGPSAVSSSWVEAISG
                     RSIPRNFEYLAEPMSQPLRVLQHHACVSGKANFSSDNIPKWCELQSDVEKRQQQREDG
                     LSWLPDAQEKLKREGQQAKIEEEQRIKPEEEQRIKLEEEQRIKLEEEQRIKLEEEQRI
                     KLEEEQRIKLEEEQRIKLEEEHKSKKYFAQSDAIDLILNNFENEISSIGAYYAPAKQR
                     AIELLDTLRKYKEEAFNDPSREKLISFAQSTKRAIQEATPILQKDLGWGDYLTNLAKQ
                     LVNAVTFAVAYAVTFGTTGHQGFFALKSSLAVSQSQNLEDALNRELSQKNC"
BASE COUNT         2780 a         1759 c         1471 g         3137 t
ORIGIN      
        1 cttgttcgcg gcatccatga gctctaatca gttgcagatt tcttcataaa gatcgcgcca
       61 ttctggattt aactcttcaa ttaaatttaa cttccactgt ctgcaccaat ttttgaagtg
      121 aaaaactggt ttgtcaggta tttccctgat tattattctg gcaaatacct gattatattt
      181 agcgataaga gattatattg gcaacttccg tcagatcctt gtttgaacga tcagcaagat
      241 catatgatcc atttaaaaga gtattacttt ctctggaaaa aaacattctt aactggtttg
      301 cattttgacg tcttaactta ggtgccatca aagggtagcc gtcattatcc agaattcgct
      361 catttattct tattgattcc tgacttatgt ctttaggcaa tttgactgcg ccggcatagg
      421 attctacaga atgtaaggta cctgtcttta aattaaaaaa ctttgacatc atttgctcct
      481 attggtttta taaatctcac tttgataata ataagaaaat gattaaaatt cactactttt
      541 attttgtgag ggttgatttt atgaacaaaa catacacaga aaagttattt tcatacggga
      601 ccttacgcta tgaaaaagta caaattgcta attttggtag aaaaatgcaa ggccaagaag
      661 atgtattgca aggattcaaa ttaaccacga ttgaaattaa aaattcagcc gttgttgcga
      721 caagtggcaa taattttcat cccattattt cgtatacggg caatccctca gacaaagttc
      781 caggtatcgt atttactatc agcaaagaag aattagcaca agctgataaa tatgaagtct
      841 ctgaatataa acgtgtgaat gtgaaattga actcaggagt gaatgcatgg gtttatatca
      901 atgctcaaga tctctatggt aataaccaaa caaaaaacta actagcaata gatattagtt
      961 cagaacaaat attatgacaa aacaaaagtg gaaagaactt cataactctc atcatcagaa
     1021 gatagaacca cactttatcc aaaggttatt gaagcataat tatgagggaa ttttaaaaaa
     1081 tgctcagggt gatgaacaag acgagtaggg ttcattaact gcttaccaat actgcaggtg
     1141 atcaccatac acgcctatat taagtctggt tattttgata atcagactta tgccaattat
     1201 agtaccgtga attcaactaa taagtgcgac tagctttttg ggcaacccaa actgtcgcgt
     1261 ctccacaatc tggatctcta attttatgcg ccagtacctt aaaattatta tctataagta
     1321 tctgttcata ttcttcggta gaaagagatg catggtataa atcgtgtcct ccattattac
     1381 tccaaacttc atcgtattca ggtcctgatg taaagattaa taaaccattt tgatcaacat
     1441 aggaagctag taactttaaa gtcattcttt gatcatcgtg aggtaaatga aaaaagctat
     1501 gccacgcaat aaccgcatgg aatttttcat tcaaatctaa agtacgcata tctgctaaaa
     1561 tccatttgtt ttttggaaat cgttgcttac acaagctaat catttttcta ctggcatcta
     1621 ttccggttac atcgtaacct tcattgatta aaaattgggc tataggttct ccagtaccac
     1681 aacctacatc taatattttg cttttaggtt tgagacattt ttgaataaaa tctaaataaa
     1741 gtttctccat agtcaaatct ttagtgcggt tatcatcaaa ccagtcaaca atctcgtcat
     1801 atacctgata aactttatct ttattctttt ccaccatgtt aaactccagt tgcaaacact
     1861 tttttatgcg ttgaatccag gtaataatag tattccaatt atattatttg ctttaccatt
     1921 atggtaattt ccaaatagta attaactctt atgctaagaa ataacattac ggatcaacac
     1981 ctcattgcgc ttttgaaagt ctattatgga attgatattc atactgtaca actcatcgtg
     2041 ggtggtgctg atatgaatgc ctttggatat aaagcggatt cagaatccaa ttcttatttc
     2101 gtaaagctga aatacggcca tcatgatgaa attaatttat caatcattcg cctcttgcat
     2161 gattctggaa taaaagaaat tatttttcct atctacacac gtgatacaaa attatttcag
     2221 caaatagatc attttaaaat aatagtgtat ccatttatta atgcacccaa tggtttcact
     2281 caaaatttaa cagaaaaaca gtggcaccag cttggaaaag tgttaagaca aattcatgaa
     2341 acttctgttc ccaccgctat tcagcaacga ttaaggaaag aaacttattc gcctaaatgg
     2401 cgtgaaatgg ttagatcctt ttataacaaa attggatttg atgattcaga tgatcaaatt
     2461 accactgact tcaaaacttt ttttaatcaa aaaatcgatt caattcatcg attagtcgat
     2521 tcatcagaag aattatctaa aaaaattcaa cttgatttag ataaatatgt gctgtgtcat
     2581 tctgatgtac atgcgggcaa tgtgttggtc gttaatgagg gatctatttt cattattgat
     2641 tgggatgagc ccatgttagc gccaaaagag cgtgatttga tgttcattgg cggtggcatt
     2701 ggtaatgtat ggaataaacc ccatgaaatc gattattttt atgaaggtta tggtaaaaca
     2761 aacgttgata aaataatttt atcttattac agacatgaac gaattgtcga agatatagct
     2821 gtatatggac aagacttact ttcacgtgat caaaacaacg agtccagact tgaaagtttt
     2881 aaacatttta aatcgatgtt tgatccaaat gatgttgttg aaatagcttt ttcttcggat
     2941 taattttaat tattttttga cgacatgctt tcaataaaat ggttggcctg tttcttggta
     3001 aaccctgtta caatgaactc atcccctatc ctgcttcgaa ttattggatt tgatatcact
     3061 ataccattta agatgaagct tccttgtttt cctataccgt tttcagtcat ttgcccaaac
     3121 ttggtagctg caatagtttt taatttaata cgtacaccat agccgtctgt tggagtttca
     3181 ggcaatacta ttgttgccga ttcaactgtt gagctatcca aaaccatctg gttttgaatc
     3241 acttgaaata ccagtgatga aatatcctta ctggcatcaa tatcagttgt tgtgggacta
     3301 gaggccattg ctgatggtaa aaaaaatact ccaacaagcg cggctattac tttaatcata
     3361 atattcatat aagaattatt ggaaaagagt aaataggtat attatatccc gtgtttgcaa
     3421 tattgaatac tttaaataca aaatgttcct tctagttgtc tcttttttcg tatttttgtc
     3481 cattgccacc aaggttgttt ttcacctgtg cgtgcctcgt ttatagcagc agcaaacaca
     3541 tcccaaacac aaggatcatg taattggcca gtaatttgtt gcaggcgtgc gtacagttga
     3601 tctgcacttg cattagcaag ttgatgaatt gaattaatcc caagcaattg caaatcctta
     3661 taagttgcct cccccacatt cataagactt aaaagtggat gggttgcctt gaacttattt
     3721 ttggtcatat ctatccctta ggctgcttat tgcaatcata ataccattga ggttggtaat
     3781 ttaagcaatt tcttgatccc atgattttaa agagtgatat tattacttct atgcatctga
     3841 ttttttattc aaaccgatta catcataaat agtgatagga gcagcaactc ccttaacttt
     3901 tactctgaga tgcccattta actcaagcga acgattaact tcctggtaag ttgcctcaga
     3961 aattaaaatt tgccccccca aagtcagatc ttggatacgt gatgccagat taaccggaga
     4021 accaattgca ctatattgca tacgtttctg agaaccaatg ttacctacaa tagcataacc
     4081 tgtattaatt cctatgccca ttgctaattc aggcaaactg ctgttgcgat tcattgtgtt
     4141 cacctcttgc atggtcgcct gcatctctaa tgcacaggct atcgctctgt atgcgtcatc
     4201 tcctccagag tgtggtgctc caaataccac cataatggcg tcaccaataa aggaatcaac
     4261 agtaccattg tatttttcta tgacatcaac catttttcca taatgattgt taagtgcggc
     4321 gacaatttta tccgcaggaa aatgctccga cagagtagta aaatttctta agtcgctaaa
     4381 caaaattgta attttgcgct cctcacctcc taattttaac tctgattttg attcaagaag
     4441 tgctgcaaca atctcgtcag acataaaact accaaatact ttacgaataa aattattacg
     4501 catttcaagc tctttcttag cttttgtcag cgaaatgttt ttttctcgta cagctctctc
     4561 aagaagcgaa atatcaagca gattgacttg atcttctgcc aaagttgcca aatcttttaa
     4621 aatttgaatt tcagtctcgt ctaaattacg tggtttttta tctattgcgc atagtgttcc
     4681 cacattaaaa ccatcaactg tagataatgg aaatcctgca taaaaacgaa tatttggtga
     4741 attagtcaca taaggattat tacaaaatcg ttcatccaac aaggcgtctg aaataatcat
     4801 cggtttattt tgctgaatag tgtagtggca aaatgaatca ttccgggata ttttgttaag
     4861 ccctatgcca cagctggatt tgaaccattg atgtttagag tcaataaagg aaataaaagc
     4921 aataggaacc gataagtaac ccgaagtgag ccgaataatt cgatcaaatc tttcgtctgc
     4981 tggtgtatcc agaattttta attcgtataa tgcagcaatg cgattctgct cagtattatc
     5041 caaaggtttc ttcataattt tctcaggtga ataaacaaaa tgagaggcat agtggttatt
     5101 aagattatag tacaaggcac acatattaat tggcttaaac caatgaataa gttaattgta
     5161 ttcagcaatg gaaataaagt tgatagctta cttgtatgtg gctaaccctt gctatgaagg
     5221 atatactcag aaacgggcaa ataaataata tttaattgcc cgtctaaagc cttaaaaatt
     5281 taacctgatt taatgatgcg agaaggtatt atcagagggc ggtattatat gttccggtgc
     5341 cttatttacc aatcctgaaa tacctgatag ctcattattt gtgaagaaac caaagcgtga
     5401 tgaaggggaa cttgaaacaa ctgataaaaa ttcactacta tgaattccat ctgtaccact
     5461 ttcagcatct ttcacaatat ttgtaagctc tttttctttt ggcgcaggta ctgagcaatg
     5521 ataaatagtt ttacaactgc cattgttatc aaggaccttt acagaacgta ttagctcaaa
     5581 tagtgtatcg aaaggactaa ccccctcttc tccttctcta aaccccaagg tatatggata
     5641 cttacatcct gcacgaactc cttcacattt agctactact acccctcgtt caccaggctt
     5701 taccttcttg catttagccc atcggccacc agccattccg ggatcatctc caattcttct
     5761 aacaatttca gtattagata atagtttact atcggcatct tcatgcaatt ctatggcaag
     5821 cattactcca ttacgtcgtg tgcctttatt gaaatatttt tgagcatatt taagatcaaa
     5881 agcaaaaaaa gtttctcctt ttgctttagt tgatatcttt tcgtcctctt cttccaatac
     5941 ttttttcttg ttaccaaata ttctctttcc ttgatttaaa ttttcaagaa ttgccaaata
     6001 ttctttttca ctgacaaagc gaaataaata attactaggt cttccagtgt taatgctgaa
     6061 ttctgcaaaa gattcttctt ctaattcgat agcatcttga atatgactgt aaagctctcc
     6121 ttctaatttc ttgatctgct ctttacgagc aatactctcc agttttttac tgttagtttt
     6181 gcttgtttct ttgctgggag aaacatccat ttcctcagaa tactcgggta caacatccaa
     6241 atcttcttta cttgattctg aaatagttaa aatttgtgtt ctttttttag gtatttgcat
     6301 tgctttactt ctacgcatct aactctccta acatcgttac gatatacaat atgtgcttat
     6361 attattctta cacgatctat tttgcacatt atcagcaaaa tgaaatggat tgcaatcttt
     6421 catttctaaa tcaaaattac cgaatcgaaa tacattagat ttaagaagaa tgcttgggca
     6481 gcaaaaataa tgtcttgccc attagcattc tatgtctggt ctattcccta acagtttttt
     6541 tgactcaatt ctctattgag tgcatcttca agattttgag attgactaac tgccagagaa
     6601 gatttcaaag caaaaaagcc ttgatgcccg gtagttccaa aagttacagc ataagccaca
     6661 gcaaaggtca ctgcattaac caattgtttc gctaaattgg ttaaataatc tccccagcct
     6721 aaatcttttt gcagtattgg tgttgcttcc tgaattgctc tttttgtact ttgcgcaaaa
     6781 gagattaatt tttctctgct tggatcatta aatgcttcct ctttatattt tctcaaagta
     6841 tctagcaatt ctatagctct ttgttttgca ggggcataat aagcaccaat agaactaatt
     6901 tcattttcga aattattcaa aatgagatca atagcatcgg actgagcaaa gtattttttg
     6961 cttttgtgct cttcttcaag tttgattcgt tgctcttctt caagtttgat tcgttgctct
     7021 tcttcaagtt tgattcgttg ctcttcctca agtttgattc gttgctcttc ctcaagtttg
     7081 attcgttgct cttcctcaag tttgattcgt tgctcttcct caggcttgat tcgttgttct
     7141 tcttcaatct tggcttgctg cccttccctc ttaagcttct cttgagcatc tggaagccag
     7201 gaaagtccat cttctctttg ttgttgtcgt ttttctacgt cagactgaag ttcacaccat
     7261 ttaggaatgt tatcagaaga aaaattagct tttccagata cacaggcatg atgctgtaaa
     7321 actcgtaatg gctgagacat aggttcagct aaatattcaa aattcctcgg gatggatctt
     7381 ccagatatag cttccaccca agatgatgag acggcacttg ggcctgagaa ttccataacc
     7441 aatggtttat agacaatccc ttgaaacgac ttcaatagtt ttttaatgcc atcgggagtc
     7501 ttaggatagt cattgagatg ttgtttttct atatgatttt caataacaga tgtaagtaac
     7561 tcttgatttt gatgctgaat tgcctcttta attacaggta taagttcttg tgtagcaaaa
     7621 gttttagcaa aatactcagc gccaaaaaaa gttgcaaacg acaaagcatc ctggtgagct
     7681 tctgaaaccg ccgcccttaa agcacataaa tcaaatttgc catctgtttt ttcaacaaat
     7741 aatttataac ctggcgaatt ttttaaacgt actaactctt cgtttttgct ttcctcaata
     7801 tatttcgcgc agttttcata acgcgcaatg atctttttgg caaccccctc gattatttga
     7861 cgacgtgcct ctttatcttt gctataagca atcatatcag tattgaaagc cgggttttcc
     7921 atagcaggtt gttgaccatc ctttgtgagc gctgacccca tattaagtag aactggaaaa
     7981 ccagaatgaa cctcaactgt gactttttta tcattcattg gtaaatcaat atcaatataa
     8041 gtactgcttt ctccaattat tacatcaacc caacgcacca gatcagatgc tgcagcagga
     8101 tttcctccct tgccctttct cattgcttca agctcaactt tggctagatt tagcaattga
     8161 gcatcagttt cactgactga ttttaaatct attgaatcta tatcaaccaa ttcaatgtta
     8221 ttttcctttg cccattgctg aaaatcaatc tgtgctgtct cattcaagca taatgagcta
     8281 aaaacaattc gagttttgcc tggctggtta agttttgcag ttttttctac ttgagtttta
     8341 acatgttgtg gcatgtaaac tgctgggtta ttgctaaacc agacagaaaa tatattggtt
     8401 gtatcaaatg cgtatttatc ttcacgacta acatccaacc ccaggatatt aacattcccg
     8461 ttgcggaggt cacttagctc tctctttaat tctggaacaa ctttttcttt taaaatttta
     8521 agttgttcct cggaaaatga ttcgctttta gtatagtcac cagtacctct tattctgtta
     8581 tataaatcgt taatctgctc ttgactaagc gctgcttcta atcgccttgc caattggtca
     8641 aatatgtaag ctcgtaattt ttgagctttc tgagtctttc catgttcaac aacttctttc
     8701 aaaagatcat ttgttaattg atttaaagcc ttattaacag gagaacggcc aactgtcatt
     8761 gcgccatata cttgaacctt gttctcaaca gactttttcg catcctgaac aggctgactt
     8821 tcaacagcat tagaaggaaa cggagtatcc aggtccttat tttttcgaac ccaatctaaa
     8881 gcaatttctt tcccaatttt aaggtagaga gcaaatttag ttaaaaacac ctcactaacc
     8941 agtgtttgtt cattaatacc agtatttaac tggtgaattt caagtaatga atcagccaat
     9001 tctttggcca tatgctcatc aacagaacat tttgatttga tattatcaat aaaaattgcc
     9061 ttttgttgct ctttcatata tttatcccac ctggttataa tctaaatttt tattgttgtc
     9121 tcaactggag aaaacaccaa aaataaa
//
LOCUS       DBNCWK010000122         9128 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.122, whole genome shotgun sequence.
ACCESSION   DBNCWK010000122 DBNCWK010000000
VERSION     DBNCWK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.122"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(48..482)
                     /locus_tag="AC06IO_002367"
     CDS             complement(48..482)
                     /locus_tag="AC06IO_002367"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIO9875540.1"
                     /translation="MNYYSILLTIKRLIIGLLPILLVACTHQQSHGPDHYVVRGKTYH
                     VMKSAKHYKAKGIASWYGSHARHQKTSTGERYNMYAMTAAHPTLPIATRVRVKNLNNG
                     RSVVVRINDRGPFLSNRLIDLSFAAAKKLGIKGIAPVEIEVV"
     gene            complement(793..2160)
                     /gene="pgtP"
                     /locus_tag="AC06IO_002368"
     CDS             complement(793..2160)
                     /gene="pgtP"
                     /locus_tag="AC06IO_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter protein PgtP"
                     /protein_id="HIO9875541.1"
                     /translation="MALLSFLKPAPYLDEIQDKNVVKRQYRYWRIRTFYAMYIGYALF
                     YFTRKSFTFVMPALQSTLGMTKTELGMIASILSLSYGISKFLSGILGDKSNPRYLMAI
                     GLILTGVFNILFGLSSTWWLFAIFWGLNGWFQGWGWPGCTKLLTHWYSQSERGRWWSL
                     WNTSHNVGGALIPLIVAVCAQYFGWRSAMFVPGIICILGGVFLINRLRDTPQSLGLPA
                     IEQYHEDCSGSANHRQEKTELSVKEILWNYVLCNQYIWILSISYLFIYIVRTAINDWS
                     MLYLTEVKEYTLITAGSCVIWFEVGGFFGNLVAGWSSDKIFQGKRNPINVLFTAGVFA
                     TLLLFTLTRAYTPFLDALFLFFFGFFIFGPQMMIGMACAELAHKKAAATATGFAGFFA
                     YCLGAVLAGAPLGAIIKNYGWDNFFLTLFVCCLIPLFLMLPLWHVKVNPKYRQSEFSG
                     KSEFA"
     gene            2338..4005
                     /locus_tag="AC06IO_002369"
     CDS             2338..4005
                     /locus_tag="AC06IO_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2552 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875542.1"
                     /translation="MKEIVSLLEEDQIDFANHKRPEVLMVLRRKFRTQKDWIITSFNQ
                     YQFTNEESQKVQQAILNGDLRITDLLNRPFSHYFWSFLTFKWGEILSGGKRLSKQEVL
                     QIASCSSNGQEFARTPTQNRNRLIKGVPLVIGKVVTSMALCMVFCLGLLSTLGAPFGF
                     SLGLAIVLSLCNGIVCLLSRGKAMLDSAGFQPQKQKTFKTLEENIKEKTILDRGQKLF
                     FGVVTLLACISAGISGYAGLVGLLTFLGAGMLISNPLVLILTIGLSVVAAVSFYSFQA
                     IGIRENYTKFKNLFIEDYQKPYGLLRCALLGMVSTVFLAVYATLTWFTTVSGVNKLFN
                     ALGATPDTLLAHFIAMICTSTTMVVNTFSQVYKVFNPKTYVDLKEKFESIYQPPEENL
                     TRAQKIQHGVINMLKLFALIGDSLAYSVAGGIRSGIHIGETLGEAIGAKFALTITMAI
                     LSPVILVFVSIAFTWPTVFDRKKSIPCPSEMDKPLIEKMSRSQTSDALQNNTLLPTSE
                     KSSKPNDSNIRQAGLRFFDVSLSEERTHKPNCNPTLESGTVLTSPSP"
     gene            4203..4877
                     /locus_tag="AC06IO_002370"
     CDS             4203..4877
                     /locus_tag="AC06IO_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875543.1"
                     /translation="MKLTASQKLRLITLNQEFKKIIRQQQQDPSSFDNHVHCLQDLEK
                     IVGCWMNFMSPYISWQIDQGNKLLDAKWNIFKQVKIKHNLEEYKKLIDEINSLFFDDE
                     LKQQMKLAFEGKPVTGFNVHIDDKEQTTRSYRGTFFEHNHLVTYKISFLDGTELTWHS
                     LEDEKEMQKARQLFERKVPQPKKEITHELAKASIKEMEAKVSTVEEENLVSNTGNDSN
                     HRCALM"
     gene            complement(5147..5977)
                     /locus_tag="AC06IO_002371"
     CDS             complement(5147..5977)
                     /locus_tag="AC06IO_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HIO9875544.1"
                     /translation="MTITTTSPQETRYIDLSSCYFYYSSRILAFITWLSCGLFGIYIL
                     FFYANSLISDSAFRWNNVLPNLYIKSHTASTLGIAIHFTAGGIILAMGFLQLLSTIRH
                     YAPGIHRWLGRIYVIAAALTGIGGLTFIASTGTIGGLVMDIGFGLYGLLMILCALQAY
                     RYARQLKFDNHQAWAIRLFALAIGSWLYRMEYGFWFLMTNKLWHTQTFHGPFDFVMAF
                     FFYLPNLLVAEAYLRGRSAQVGQFSKLIAGTVINLASCLLAVATYFFATQYWLPAICA
                     "
     gene            6105..6674
                     /locus_tag="AC06IO_002372"
     CDS             6105..6674
                     /locus_tag="AC06IO_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875545.1"
                     /translation="MNLMLRKWIGLIAAFLFLNTGFVQASATPIICDQEYTLCTSARC
                     IPMPGNPAKAVCDCVVEKGKSAGYKTCKERKPIRDRYKVTSLVSTFSFEQFAFKRKMN
                     CSEGLPWTNCLDMPCTVDPQNSKRALCLCTIENTQAFFTFGGDCNTNTCATGFWSGAT
                     QENSIILRKALMQEMRLKPKALLKDCSAK"
     gene            6990..7745
                     /locus_tag="AC06IO_002373"
     CDS             6990..7745
                     /locus_tag="AC06IO_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIO9875546.1"
                     /translation="MLLTNRIDLRSYHTESCSHTHDFAQLVLPVSGSMELEVGHYSGV
                     VNVDIGVYIAPNEQHCFAGSQKNLFLVIDATTQNSLRDEAFESNILNLTASTKKLIQF
                     THHYLAHNERDFFTDSLINQLLFHFVANSFSPEPDPVVIRAKQWIDLYFADSVDVSRV
                     AKHCYLSISQLQRRFKQMLGCGIAEYWRMKKLLHAKKLLSQKNCSIEAIAFEVGYENL
                     PAFSRRFSKVFGESPSQWRAKALTAKKMREIDK"
     gene            7811..8725
                     /locus_tag="AC06IO_002374"
     CDS             7811..8725
                     /locus_tag="AC06IO_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIO9875547.1"
                     /translation="MVIDVKKSNTYFIQGMLFLIFAQIMVGINIVFSKYVLSSIPIIY
                     ILALRFTLAAVILLLLHWLTPAKKLPVYSYFLQLKRRDWFFIFAQALSAGVLFNFLIL
                     WGLQYTDANVAGIITSALPAIIAIMSWLILGEKISGKKAICVGFATLGLVVIACDKLG
                     DINVSHSFLGDALVLTSLLPEAMYYILSKMHVNSLPVFLISSLLNGINAILLLFCLSF
                     STWDSFTIHTLDWLILIILGLSSGLFYVFWYFGCQTVDGVMASLSTAVMPLATVTIAW
                     IFLGEQLTIGQIIGMGMVILSITVYAKR"
     gene            8735..9070
                     /locus_tag="AC06IO_002375"
     CDS             8735..9070
                     /locus_tag="AC06IO_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HIO9875548.1"
                     /translation="MTISYEEFEKVDLRSGTVVKAESFLRARKPAYKVWVDFGADIGV
                     LQTSAQITVHYTPEELIGRMVIGCVNLGEKNIAGFLSQFLLLGFSDEKGAICLASVDP
                     HVPNGKKMH"
BASE COUNT         2935 a         1671 c         1786 g         2736 t
ORIGIN      
        1 aggtaaatca ggttattagc ttgagtaagc tttacttctt agcattttca aacgacttca
       61 atttcaacag gagcaatgcc ttttataccg agttttttag cggcagcaaa ggataaatca
      121 attagcctgt ttgataaaaa aggacctcta tcgttaattc gaaccacaac cgatcggcca
      181 ttattcaggt ttttgacccg tactcgtgta gcaattggga gtgttggatg cgcggctgtc
      241 atcgcataca tgttgtagcg ctctccggtc gatgtttttt ggtgccgagc gtgtgaccca
      301 taccaggaag caattccttt tgctttgtag tgtttggcag acttcatcac atgatatgtt
      361 ttccctctga ccacatagtg atccggacca tgagattgct gatgcgtaca agcaaccagt
      421 agtatgggga gtaaacctat aatgagtcgt ttaatggtca acaaaatcga ataataattc
      481 ataaagttat cttctacttc cgctacagat tttagatata cgtaattcat agatcacgag
      541 aatcaaagat atttcttgca aagaaaatcg tcaataggta acacattttt atcatttgct
      601 tccatcattg atttgattga ttatacattt tttggatgaa tatgtaccaa caaatttttc
      661 tgtataacct cttgtcctat cacaacaagg atatctctat accccagggg ctttcatgtg
      721 gaggtagcaa gagcagatcg ggccaaggca ggcccgacct actttttctg gattacccag
      781 ggaagccaat cactaagcaa attcggattt gcctgaaaat tctgattgac gatactttgg
      841 attaactttc acatgccaga gcggcagcat caaaaataat ggaatcaggc agcaaacaaa
      901 caatgtgagg aagaaattat cccagccgta atttttgata attgcaccca aaggagcacc
      961 ggccagaaca gcacctaaac aataagcaaa aaatccggca aagccagttg cagtcgccgc
     1021 tgctttttta tgtgctaatt ccgcacaggc cataccaatc atcatttggg gcccaaagat
     1081 aaaaaaacca aagaaaaaaa gaaacaaagc atcgaggaac ggtgtatatg ccctggttaa
     1141 cgtaaacagc aataaagtgg caaatacgcc tgcagtaaaa agaacgttga ttggattgcg
     1201 tttgccttga aatattttgt cagaagacca gccggcaacc agatttccaa aaaagccacc
     1261 aacttcaaac catatgacac aacttcctgc agtgattaag gtatactctt tcacttccgt
     1321 taaatacaac atgctccaat cattaatggc cgttcggaca atgtaaataa acaaataaga
     1381 aatggacaat atccaaatat attggttgca caacacataa ttccaaagaa tttctttgac
     1441 tgagagttct gttttctcct ggcggtgatt tgcggaacca gaacagtctt catgatattg
     1501 ctctatcgcg ggtaaaccaa gtgattgtgg ggtatctctt aaacgattga ttaaaaaaac
     1561 acctcctaaa atgcaaatga tcccaggcac aaacatggct gaacgccagc cgaagtattg
     1621 cgcgcacaca gcaacaatca aaggaattaa tgcaccacca acgttgtgtg atgtattcca
     1681 aagactccac caacggccac gctccgattg agaataccaa tgtgttaata atttagtaca
     1741 tcctggccag ccccaccctt gaaaccaacc attcaatccc caaaaaatag cgaataacca
     1801 ccaggtagaa gacaatccaa acaaaatatt aaaaacacct gtcaaaatca gtccgatcgc
     1861 cattaaataa cgaggattag atttatcacc aagaattcca ctcaaaaatt tgctgatacc
     1921 ataacttaaa ctcaaaatgc tggcgatcat tcccagttca gttttagtca tccccaaggt
     1981 gctttgtaac gcaggcatca cgaaggtaaa gctctttcgc gtaaaataaa acaacgcata
     2041 ccctatatac attgcataaa aggtgcgaat acgccaataa cgatattgcc gtttaacaac
     2101 gttcttatcc tgaatttcat caagatatgg agcaggtttt agaaaactca ataatgccat
     2161 gattgtccat gatcatgaaa aaaacatgat tagaatgtaa ctcgcaaagt atgtcaaatc
     2221 tttctagtga aaatttaaca ggatcatacc tattgatgat tgaaataaaa atttaatgga
     2281 attcttgtga atttataaat aagctacgca gtttgtatta aaaaaggatt tattgtaatg
     2341 aaagaaatag ttagtttatt agaagaagat caaatcgact ttgctaatca taaacgtccc
     2401 gaagtgttaa tggttttaag aagaaaattt cgtactcaaa aagattggat tattacctct
     2461 ttcaaccaat atcaatttac taacgaagaa tcgcaaaaag ttcaacaagc cattctaaac
     2521 ggggatcttc gcataacaga tcttcttaat cgtccttttt ctcactattt ttggagtttt
     2581 cttaccttta agtggggaga gatactttcg ggtggaaaaa gattgtctaa gcaggaagtc
     2641 ttacagattg ctagctgctc tagcaatggc caggaatttg cgagaacacc aactcaaaat
     2701 agaaacaggc tgataaaagg agtgccattg gtcattggca aagtcgttac ctccatggca
     2761 ctgtgtatgg tattttgtct gggattatta agtactttag gagcgccttt tggctttagc
     2821 cttggcttgg cgatagtact ttccttatgt aatggaatag tttgcttatt aagccgcggg
     2881 aaagccatgc ttgatagtgc cggctttcag cctcaaaaac aaaaaacgtt taagaccctt
     2941 gaagaaaata taaaagaaaa aacgatactc gatagaggcc agaaattatt ttttggtgtc
     3001 gtgaccttgt tggcgtgcat ttctgcgggc atttctggat atgctggtct tgtaggactt
     3061 ctcacgtttt tgggggcagg catgctaatc tctaaccctc tggttttgat acttaccatt
     3121 gggttgtctg tggttgctgc ggtctctttt tattcgtttc aagctatcgg catacgtgaa
     3181 aattatacaa aatttaaaaa tctttttatt gaagattacc aaaaacccta tggtcttttg
     3241 cgatgtgctt tattaggaat ggtaagtacg gtttttttgg ctgtttatgc gacgttaact
     3301 tggtttacaa cggtctcagg ggttaataaa cttttcaatg ctttaggtgc gactcctgat
     3361 actcttttag cacattttat tgccatgata tgtacttcaa caacgatggt tgtcaatact
     3421 ttttcacaag tatataaagt ctttaacccg aaaacgtatg tcgatctaaa agaaaaattt
     3481 gaatcaatat atcaaccacc tgaagaaaac ctaaccagag cacaaaagat acagcatgga
     3541 gttatcaaca tgctcaaatt atttgcttta ataggtgatt ctctcgctta ttctgttgcc
     3601 ggtggaatcc gaagcgggat tcatattggt gaaacgctgg gagaagcaat aggggcaaaa
     3661 tttgcattaa ccattaccat ggcaatattg tctccggtca ttttagtatt tgtatcgata
     3721 gcttttacct ggcctactgt ttttgataga aaaaaatcga ttccatgccc ttcagagatg
     3781 gacaagccat tgatagagaa aatgagtcgc agccaaactt cagatgcgct gcaaaataat
     3841 actctattac caacatcaga aaaatcatcc aagcctaatg attcaaatat caggcaagca
     3901 gggctaagat tttttgatgt gagcttgagc gaagagcgta ctcataagcc taattgtaac
     3961 cctactttag agagcggaac cgttttgaca tctccatcgc cataagtgat gaggtttttg
     4021 aatcaactag tgaagtatcg attgatttga atctggaaaa gaaagaattc aatcgatact
     4081 tattaaataa ccaatttgca ggctctctcc ttttatattt tagaattgtc ggatatccaa
     4141 attgcaatca gatttcatat ttaatactat catgtgtcaa aagtaatata aggatatatc
     4201 ccatgaagct tacagcatct caaaaactaa gattgataac tttaaaccaa gaatttaaaa
     4261 agattattag acagcagcaa caggacccca gtagttttga taatcatgta cattgcttac
     4321 aagatcttga aaagatagtc gggtgttgga tgaattttat gtctccctat atttcttggc
     4381 aaattgatca gggaaacaaa cttctggatg ctaaatggaa tatatttaag caagtaaaaa
     4441 taaagcataa tttggaagaa tataaaaaat taattgatga aatcaattct ttattttttg
     4501 atgacgaatt aaaacagcaa atgaaattag cgtttgaagg taaaccagtt acagggttca
     4561 atgtacatat agacgataag gagcaaacaa ccaggagtta ccgaggaaca ttttttgaac
     4621 ataatcattt agtgacttat aaaattagtt tcttagatgg tacagagcta acatggcatt
     4681 ccttagaaga tgaaaaagaa atgcaaaaag cccgacaatt atttgagagg aaggtacctc
     4741 agcctaaaaa agaaattacc catgaattag ctaaggcatc aataaaagaa atggaagcta
     4801 aagtgtctac agtggaagaa gaaaatcttg tatcaaacac aggcaatgac tctaatcata
     4861 gatgtgcgct tatgtaaaat tgatagtatg ctcaaaagga agaataacct gatgctcaat
     4921 tagaggtgtg atgggattga atgcagtact tattaatccg gtaaaaaaag attggtaagt
     4981 aaggttattg atagacctat atttaagttt tcatagttct gtgcgcgaaa acattctacc
     5041 gaaaaattag ggcgtggaat cattaaatac aaattttggt aaataagaca gagctggcta
     5101 aatagaaatc ctggttgcaa gcaaccagac taggtttact tttttgttaa gcacaaatcg
     5161 ctggtaacca atattgcgta gcgaaaaaat aggtggctac cgctaataaa cagctagcca
     5221 agtttatcac ggttccagca atgagtttgg aaaactgacc aacttgtgcc gagcggcctc
     5281 gcaaataggc ttcagcaacc aacaaattgg gcagatagaa gaagaatgcc atcacaaaat
     5341 caaaaggacc gtgaaatgtt tgggtatgcc atagtttgtt tgtcatgaga aaccagaaac
     5401 catattccat cctgtagagc catgagccga tcgcaagtgc aaacaaacga atagcccaag
     5461 cctgatggtt atcgaatttt aattgtctag cataacggta agcctgtaag gcacacaaaa
     5521 tcattaataa tccataaaga ccaaaaccaa tatccattac cagcccaccg atagttccgg
     5581 ttgaagcgat gaaagtcaag ccgcctattc ctgtaagagc agcagcaatg acatagatgc
     5641 gccctaacca gcggtgtatc ccgggtgcgt aatgacgaat ggtgctgagt aactgtaaaa
     5701 atcccattgc aagaataata ccgcctgcag taaagtgaat ggcaatacct aaagttgatg
     5761 cggtgtgcga tttgatataa agattaggta atacattgtt ccaacgaaag gcagagtcgg
     5821 atattagaga gtttgcgtaa aaaaaaagta tgtaaattcc gaataaaccg caactaagcc
     5881 atgtaatgaa agcaagaata cgagaggagt agtaaaaata acaagaagat agatcaatgt
     5941 atctcgtttc ttgaggagaa gttgtagtta ttgtcatgtg ctcaataatt aatcgtagtg
     6001 ggatcagagt tagtctacaa tgtctagtac tctatttaaa gctcatacga ttcgatagta
     6061 gtataaatat gctgctataa ataaaaaata aagagatctg aaaaatgaac ttgatgctta
     6121 gaaaatggat tggattaata gctgcttttt tatttctcaa tacggggttt gtacaggcct
     6181 ctgcaacgcc aattatatgt gatcaggagt atacgctttg cacttccgct cgttgtattc
     6241 ccatgcctgg aaatccggcc aaagcagttt gtgattgcgt agtagaaaaa ggtaaaagtg
     6301 ccggttataa aacatgtaaa gagagaaagc ctattagaga cagatacaaa gtgacgtcac
     6361 ttgtttcaac tttttcattt gagcaatttg catttaaaag aaagatgaac tgctcagaag
     6421 gattaccttg gacaaattgc ctagacatgc catgcacggt tgatccacaa aattcaaaac
     6481 gtgctttgtg tctttgtacg atagagaaca cccaagcttt ttttactttt ggcggtgatt
     6541 gtaatacgaa tacctgtgct accggctttt ggtctggtgc aacccaagag aatagcatca
     6601 ttcttcgtaa ggcattaatg caagaaatgc gtttgaaacc caaggcacta ctcaaggatt
     6661 gctctgctaa atgaggataa gcaaactaga gggagttgat taaaatttag attgatttaa
     6721 attagtttcg acttaatctg acacaactac ttgaaaaagt gagatgctca ctacaacaga
     6781 gtctagccct gggcgatccc gttgcctatt ttcaataaac tctacccagg cgtttaaagc
     6841 ctttgttagt cctgacctat ccgtgtgata aaaaactcaa gctgatccaa ataacgatat
     6901 ctaatttcct gactacccct ctcaatggtt tcaaatcaag tatgatataa tctgtttctt
     6961 ttaaaggtaa atgctcgctt gggagcaata tgttgctgac taatcgtatt gatcttcgtt
     7021 cttaccatac tgaaagttgc agtcacactc acgattttgc gcaattggta ctgcccgtct
     7081 cgggttctat ggagctagag gtaggtcact actccggtgt cgttaatgtt gatatcggag
     7141 tatacattgc gcctaatgaa caacattgtt ttgccggaag tcaaaaaaat ctgtttttag
     7201 tgattgatgc aactactcaa aactctcttc gtgacgaagc ttttgagtct aatattctca
     7261 atttaaccgc cagtactaaa aagctcatcc aatttaccca ccattattta gcacataatg
     7321 aaagagattt ttttacagac tctttaatca atcaattatt gtttcacttt gtggcaaatt
     7381 ctttttcacc cgaacctgat ccggttgtta tcagagcaaa acaatggatt gatctttatt
     7441 ttgcagattc tgtggatgtg agcagggtag cgaaacattg ttatttaagc attagtcaat
     7501 tgcagcgccg ttttaaacag atgttaggct gtggtattgc cgaatattgg cggatgaaaa
     7561 aattactcca tgctaaaaaa ttgttatctc agaaaaattg ttccattgaa gcaatcgcat
     7621 ttgaagtagg ttatgaaaac ttgcccgcat ttagtcgccg ttttagtaaa gtattcggtg
     7681 aatccccttc tcaatggaga gccaaagcgt taacagcaaa gaaaatgcgt gaaatagata
     7741 aataagctct ggttttaaat tgatagacta cgaattcatt tgatggtatc gattattaaa
     7801 taagacctag atggttattg atgtgaaaaa atccaatact tattttattc agggcatgct
     7861 ctttcttatc tttgcccaaa ttatggtagg cattaatatt gtattttcaa aatacgtact
     7921 ttcgtcaatt cctattattt acatactggc gcttcgtttt acactggctg ctgtcatttt
     7981 attactgtta cattggctaa ccccagcgaa aaaattaccg gtgtattctt attttttaca
     8041 gttaaaaaga agagattggt tttttatttt tgctcaggca ttatctgcgg gagtactttt
     8101 taatttcctt atattatggg gcttacaata tacggatgct aacgtagctg gcattataac
     8161 gagtgctttg cccgcaatta ttgctatcat gtcgtggcta attcttggcg agaaaatatc
     8221 aggcaaaaaa gccatttgtg tgggttttgc tactcttgga ttagttgtaa ttgcatgcga
     8281 caagctaggc gacatcaacg taagccattc ttttttaggt gacgcacttg ttttaacatc
     8341 gcttctgcct gaagccatgt attatatttt aagtaaaatg cacgttaatt cattacctgt
     8401 atttttaatt tcttccttgc tcaatgggat taatgcgatt ttattgttat tttgcttaag
     8461 ttttagcaca tgggatagtt tcactatcca tactttggat tggctaattt taattattct
     8521 gggattaagt tccggccttt tttatgtttt ttggtatttc ggttgccaga cagttgatgg
     8581 agtcatggct tcgttatcga ccgctgtaat gcctttggca acagtgacta ttgcctggat
     8641 atttcttggc gagcaattaa cgatagggca aataattgga atgggcatgg ttatactttc
     8701 aattacagtc tatgcaaaac gttgaggtgg aaaaatgacg atatcttatg aagaatttga
     8761 aaaagtcgat ttgcgttccg gtaccgttgt gaaagcagaa tcattcctgc gtgccaggaa
     8821 acctgcctat aaagtttggg tggattttgg tgccgatatt ggcgttctcc aaacctctgc
     8881 gcaaataaca gtgcattata cgccggaaga attgataggg cggatggtca ttggatgcgt
     8941 gaatttaggg gagaaaaata ttgccgggtt cctctctcaa tttctattgc ttggtttttc
     9001 cgatgagaag ggtgctatat gtctggcttc cgttgatccc catgttccta atggtaaaaa
     9061 aatgcactga ttcctatctg aaatattgac acatcatcat tatcaactat ggatttgttt
     9121 ttagctcc
//
LOCUS       DBNCWK010000123         9029 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.123, whole genome shotgun sequence.
ACCESSION   DBNCWK010000123 DBNCWK010000000
VERSION     DBNCWK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.123"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            234..4838
                     /locus_tag="AC06IO_002376"
     CDS             234..4838
                     /locus_tag="AC06IO_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidE phosphodiesterase domain-containing
                     protein"
                     /protein_id="HIO9875549.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGHGDITSGSIYNGVPTIDTEAL
                     NRQGFMPVLTGVGPKRDSGHWIMLIKGPGNQYFLFDPLGKTSGEGYKNTLLAQLPIAS
                     TLSVIPNEPGLNKGLCGYWVASVGLKARAELNKDNPPDLETLGRTTTEEMRNELTDNG
                     YLKITGWLKAVADKFPEGDPQPDAKALRQTTEKDLHIELPSLVSPVKDTAPKEISTKP
                     TAPQIALKHSLDSKLLENDDDVLDTIKYVHKEYLGKPYPGPLKNPKAPEEGRLPPNEG
                     PDRGPHGLAHTLRTMACAEVMIEEARKAQLRGETLGKAKNGQTLADVTPEELKKILIA
                     QAFFVVGRDDERSGYDDVHKRNFYAEYHEKSEQAFRKYVEDNKLIGKIFKDQKEVDFY
                     AAIILDKNHEWDASPAHILINQGHMVDLMRTKAPAEVALERTYNTLKYTLGSQGAEVV
                     LKAHRDFFFVTGAVVPLVNPEAIDDPSRGGPYENPYSGEKFVIVDDKVPASKKDLPKA
                     VNRDYKLKDNERFLTIKEYYAFPDVQQTYPGYKTRLEGSSYYFPTPFAGECEQDPAKC
                     LGAIQKARSKLQTDAIKNGFQSSSDKERRQPNMDEIAAARIIQQIMANPDCIHDDHVL
                     INGQKLEEKFFRDLLAKCDMAVVGSLLNDTDVKNIDTLMRHEKDTEFHSTDPQAVPVK
                     IGDAWENRIRKKGGDVTQMKHDLIFLMQNDAWYFSRVNAIAQNRDKGSTFKEVLFTTL
                     MTPLTNKSLMDTSHVPAPKKLYRGLNLPQEFTNKLINQANTIIANTENTLFTDLSTET
                     FKQIKLNDFSQMSGRTCASTTKNMKLLTDIWGSNVIFEMLDPDGLLHPKQVGTHMAGS
                     EDEFSVYLPEDVALVPTKVTLEGKTDTGEDRYIFTLVAVKSPDFIPRHESGYAVEPFM
                     KMQKEKVTQALDAIEKEKGSYNIDEQLKNLRTEMVRQAKLPLREGVFDRLSHRLSLET
                     SDNKISPERRDFLNQHVIPVLQECHIALRTNNMDMMQKALAKFPTDKQWSAFKSGEAV
                     RAKAQIDVLKQQIEKTISLQSQIIPALTECSEALDKQNPTEALQALNKLPTEKEIAKA
                     KGIGQELRGQITGVKQELTGNLEPLQRAVTTPVVKDAEKMRVRYETLVTDVTKRVTDF
                     EKIKPANLESYNKAIADLNNIQQELNLLRNEKIRMHTDKDKAVDFSDIEALEKRLQDT
                     QPKLLTQLFEQTSKDIAKVKIPEKLTFTIIKSMTSKIKEQMDILELVRNERIKQHGTS
                     TEPLDMSDLDKLKGQLQTLSQSLVDDLLEDIKDSLSNIKDIATFEEESKYIEKCLDHL
                     TGLEQTLDGSEKAIKQKEDISTYRKALIDKQEKAYPEIVQLQYKSEGLIMQLRDLCNV
                     HHENLAEARKVRLQQLDNQNSGGGLLGGLWTVTNTLGLTTDTVHVEKMQIRMKEQVLR
                     GFKTGLNNDKQDANQIIAFLAKKKPSELEEGLGISKENATELHGLLQQLDSKTASIDK
                     LQENARLIDEISTKIGKEPVKQDHTITMVEEESDDIRYGL"
     gene            complement(4960..5004)
                     /locus_tag="AC06IO_002377"
                     /pseudo
     CDS             complement(4960..5004)
                     /locus_tag="AC06IO_002377"
                     /inference="COORDINATES: protein motif:HMM:NF016529.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            5053..6174
                     /gene="dupA"
                     /locus_tag="AC06IO_002378"
                     /gene_synonym="laiE"
     CDS             5053..6174
                     /gene="dupA"
                     /locus_tag="AC06IO_002378"
                     /gene_synonym="laiE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase DupA/LaiE"
                     /protein_id="HIO9875550.1"
                     /translation="MPIILDPEVLKVAEYVYQERLSKPYTEVGPEWEYNHKNPYATRA
                     TGTGHNLQRFITIDDQKLHRPIHGLAHTMRTLFYSQLMYEAAKRQPHPHRCADGRTIA
                     DLSVEDLKKLNIAQLFFVAGRESEASYGDAYHRYHLYGAKQFEEYARKHLTHLFSEKE
                     IVLYSRCIEDRIGDRFDETAEGYLIHLSHMIDLMRCKSPVEVFIGHSRGVSGIVPTLI
                     QLFGREDGLAIMHYARSLFAATGEAVPYISSSEWPHLGIESDRVERALKIVGPLEVEG
                     QEADAKKTAQAGFSVDGCYGALVKVDTPDWYHQVKEKEDYDVDEVIALPPQITIREEA
                     PKANQPFLLSLLRHVFWCCPSTQKRDDENTDVLEKKAQL"
     gene            6227..8851
                     /gene="sidJ"
                     /locus_tag="AC06IO_002379"
     CDS             6227..8851
                     /gene="sidJ"
                     /locus_tag="AC06IO_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS meta-effector polyglutamylase SidJ"
                     /protein_id="HIO9875551.1"
                     /translation="MFGFIKKVLELFGVDQSEDNPSETVVETTDVSTKIKTTDTTQEE
                     SSVKTKTVVPTQPGSSVKPETIAPDQQKKHQIKTETTTGTTTQKSPKVTLMDGHVKQY
                     YFARRGETSTHDTSLPPPVKVLSGRSIPLKEIPFEATRNELVQIYLTSIDQLIKSNKL
                     GSVPSQQLASHYLFLRSLANSETDGIKKNQILSLAKPLGAYLASKEPHVWKMINELIE
                     KSEYPIIHYLKNNRAHSNFMLALIHEHHKGSLTKNQSAFIQKFRDSSVFLFPNPIYTA
                     WLAHSYDEDSSFNPMFRERLSTNFYHSTLTDNLLLRTEPKEVTLSSEHHYRKEKGPID
                     SSLRYQMSSDRLLRIQGRTLLFSTPQNDVVAVKVQKKGEPKSTLEEEYQMADYLLKHQ
                     HRLDLQSELPQPLGQYSVKKSEILEISRLSPNSERFRDLIDDAKDLEVYVYKAPLSYF
                     TYLHDEHQNLEQLTTSVKTNVHDLFVLLREGIVFPQLADIFHTHIGEDEREDKGRYQS
                     LVQLLNVLQFQLGRIDKWQKAVEFVNLRGSGLADLGDSLPITSLFTSSDFTKHYFSEL
                     LTGGYHPTFFDKSSGTANSLFTGKRRLFGNYLYLNTIAEYLLVIQLTLGSYGDKVTRD
                     MMDKPKKEAVWRELANVMFSSCAETIHIMTGIPQSRALTLLKQRANIEKHFRQTQFWM
                     TPDYSKLDEDTLQMEQYSIYSGEPEYEFTDKLVSGVGLSVDGVHQDLGGYNRESPLRE
                     LEKLLYATVTLIEGTMQLDKEFFKQLQQVEKILSGEIKTDASSCFEAVAQLLDLARPG
                     CHFQKRLVLSYYEEAKLKYPSAPTDSYDSRFQVVAKTNAAITIQRFWREARKNLSENS
                     DIDSEKPESERTSDKRLQ"
BASE COUNT         3090 a         1853 c         1766 g         2320 t
ORIGIN      
        1 aatatcagcg cattaaactc ttatatcgtc gtatcaattc atcccggctt gtttttaaaa
       61 tagcaagtcc ttactgtctc ttaattctct aattagaaaa aacaagagcc taatttgaaa
      121 cacttaatat ttccttaaaa ctattatcct ataataagta tacaaagtgt gaatattcgt
      181 atccattaga atcagtattt gcatgtacaa acatcagttt gggagaaagt aaaatgccta
      241 aatacgtaga aggggtagaa ttaactcaag aaggtatgca tgctattttt gcacggatgg
      301 gacatggcga tatcaccagc ggtagcattt ataatggagt accgactatt gacacagaag
      361 ccctgaacag gcaaggcttc atgcccgttt taacaggagt aggccctaaa agagactccg
      421 gtcactggat catgttaatc aaagggcctg gtaaccaata ttttctcttt gacccactgg
      481 gtaaaacatc aggcgaaggc tataaaaata ctttattagc ccaattaccc atagcctcta
      541 ctctttctgt tatccccaat gaacccggcc taaacaaggg gctatgtggc tattgggttg
      601 cttctgttgg gctaaaagct cgtgctgaac tgaacaaaga caatcctcct gatttagaaa
      661 ccctggggcg aaccacgaca gaggaaatga gaaatgaatt aaccgataac ggttatctga
      721 aaattaccgg ctggctaaaa gctgtcgctg ataaatttcc agaaggcgat ccccaacccg
      781 atgcgaaagc attaagacaa accactgaaa aagatttgca catagagcta ccctctcttg
      841 tttctcccgt gaaagacaca gctccaaagg aaatctctac aaaacctact gcaccacaaa
      901 tagccctcaa acattcctta gacagtaaat tattagagaa tgacgatgat gttctagaca
      961 ccataaaata tgttcataaa gagtatttag gaaaaccata tcctggccca ttaaaaaatc
     1021 caaaagcacc ggaagaagga cgacttcctc caaatgaagg gcccgatcga ggacctcatg
     1081 gtcttgctca tacgttaaga acaatggctt gtgccgaggt gatgatagaa gaagcacgta
     1141 aagctcaact aagaggagag acgttaggta aagcaaaaaa tggtcagact ttagctgatg
     1201 taactccaga ggaactgaaa aaaattctca tcgcacaagc cttttttgtg gtaggaagag
     1261 atgatgaacg ttccggctat gatgacgtac acaagagaaa tttttacgcg gaatatcatg
     1321 agaaaagtga gcaagcgttc agaaaatacg ttgaagataa caagctcatc ggcaaaatct
     1381 ttaaagatca aaaagaagtc gatttctatg ccgcgattat tcttgataaa aatcatgaat
     1441 gggatgcctc cccggcacat atcctgataa atcaaggcca tatggtagac ttaatgagaa
     1501 ctaaagcacc tgctgaagtc gcattggaac gtacctacaa tacgcttaaa tatactttag
     1561 gttctcaggg tgctgaagtc gtcctcaagg cacatcgaga ttttttcttt gttacaggcg
     1621 ctgttgttcc cttagtaaac cctgaagcaa ttgatgaccc cagtagaggc ggaccttatg
     1681 aaaatcctta tagcggggag aaatttgtta ttgttgatga taaggtacca gcttccaaga
     1741 aagacttgcc aaaagctgta aatcgtgact ataaactgaa agataacgag cgattcctaa
     1801 ccataaaaga atactacgct tttcctgatg tgcagcaaac ctaccctggt tacaaaacgc
     1861 gtttggaagg aagctcttac tatttcccaa caccatttgc aggagaatgc gagcaagacc
     1921 ctgcaaaatg cctgggggct atccaaaaag cccgttcaaa actacaaacg gatgccatta
     1981 aaaatggctt tcaatccagc tctgataaag agagaagaca acccaatatg gatgaaattg
     2041 cggccgcccg catcattcag caaatcatgg ccaatcctga ttgcatccat gacgatcatg
     2101 tgctcatcaa tggccagaaa ctggaagaaa aattcttccg tgacttatta gccaaatgcg
     2161 atatggctgt tgtaggctcg ctgctcaatg acaccgacgt caagaatata gatactttaa
     2221 tgcggcatga gaaagacacc gaatttcatt cgacagatcc tcaagcagtt ccagtgaaaa
     2281 ttggagacgc ttgggaaaat agaataagaa agaaaggtgg cgatgtcact caaatgaagc
     2341 atgatttaat tttcctcatg caaaatgacg catggtactt tagccgagtc aacgccatcg
     2401 cacaaaaccg ggataaaggc tccactttca aagaggtgct gtttaccacc ttaatgacgc
     2461 cattgactaa taaatcatta atggatacat ctcacgtccc agctccgaaa aaactgtatc
     2521 gtggattaaa tctgccacaa gaatttacca ataaattaat caatcaagcc aatacgatca
     2581 ttgccaatac agaaaatacg ctgtttactg atctttctac tgaaaccttc aaacaaatca
     2641 agttaaatga cttcagccaa atgtcgggca gaacctgtgc cagtacaacc aaaaatatga
     2701 aacttttaac agatatatgg ggctccaatg tcatttttga aatgcttgac ccggatggtt
     2761 tactacatcc aaaacaagta ggaactcata tggctgggtc agaagatgaa ttttccgttt
     2821 atttaccgga agatgtcgct ttagtaccaa ccaaggtaac tcttgaagga aaaacagaca
     2881 caggagaaga tcggtatatc tttacactgg ttgctgtaaa aagccctgac tttatcccac
     2941 gacatgaaag cggctatgcg gttgagccct tcatgaaaat gcaaaaagag aaagtcactc
     3001 aggcattaga cgccattgaa aaggagaaag gcagctataa catcgacgaa caactaaaaa
     3061 atctaagaac agaaatggtc aggcaagcca aattgcctct tagagaaggg gtttttgata
     3121 ggctctctca tcgcctttct ctggaaacca gtgataataa aatatcaccc gaacgcagag
     3181 attttcttaa ccagcacgtc atccctgtat tacaagaatg ccatattgct cttagaacaa
     3241 acaatatgga tatgatgcaa aaagccttgg caaaattccc taccgataag caatggtctg
     3301 cttttaaatc aggagaagcc gttagagcca aagctcaaat cgatgtgtta aagcaacaga
     3361 ttgaaaaaac aatcagtctg caaagccaga ttatccccgc cctcaccgaa tgtagtgaag
     3421 cactggataa acaaaatcct acagaagcat tgcaagcgct caataaactg cccacagaaa
     3481 aagaaatagc taaggctaag ggaataggac aagagttaag agggcaaatc acgggtgtca
     3541 agcaagagct aactggaaat cttgagcccc tgcaacgcgc agtcactacc cctgttgtaa
     3601 aagatgctga gaaaatgaga gtacggtatg aaacccttgt cacagacgta accaaacgag
     3661 tcaccgactt tgaaaaaatc aaaccggcca atcttgaaag ttataacaaa gccattgccg
     3721 atttaaataa catacaacaa gaattgaacc ttctgcgtaa tgaaaaaatt cgcatgcata
     3781 ctgacaaaga caaagcagtg gacttttctg acatagaagc attggagaaa cgattacaag
     3841 atactcaacc caaattgtta actcaacttt ttgagcaaac gtccaaagat atcgcaaagg
     3901 taaaaatacc agagaaactg acctttacaa ttatcaagtc aatgacatcc aaaattaaag
     3961 agcaaatgga tatattagag ttggttcgta atgaaagaat aaaacaacat ggaacatcca
     4021 cggaacctct cgatatgtct gatttggata aattaaaagg ccaattacaa acattgagtc
     4081 aatccttggt tgatgatttg ttggaagaca ttaaagactc tttgagtaat atcaaagaca
     4141 tcgctacttt tgaggaagaa tcaaaataca ttgaaaaatg tcttgatcat ttgacagggc
     4201 ttgaacaaac gttggatggc tcagaaaaag caataaagca aaaagaagac atcagcactt
     4261 atagaaaagc cttaatagat aaacaggaaa aagcctatcc agaaatagtg caactgcaat
     4321 ataagagtga agggctaatc atgcaattac gtgatttatg taatgttcat catgaaaatt
     4381 tagccgaagc aagaaaagtg agacttcaac aactggataa tcaaaatagc ggaggagggc
     4441 tcctgggtgg gttatggacc gttaccaata ccctgggtct tactacagat actgtacatg
     4501 ttgaaaaaat gcagataaga atgaaagaac aggttcttag aggatttaag actggtctca
     4561 acaatgacaa acaagatgca aatcaaatca ttgccttctt agcgaagaaa aaaccatcgg
     4621 agttggaaga aggtttaggc atatcaaaag agaatgcaac agagttgcac ggactactac
     4681 agcaactgga ctctaaaact gcctcaatag ataaattgca ggaaaacgca agattaattg
     4741 acgaaatctc aactaaaata ggcaaggaac cagtaaaaca agatcatacc atcacaatgg
     4801 ttgaggaaga aagtgatgat ataaggtatg gtctataagg aggttgcatt taagtgcttg
     4861 ttagattgtt aaataattta atgtatagtt caaagctgtg gcagatgaga aagcaaaaca
     4921 tcatattatt tgtttctcat caatctgctg ctttttattt taaggaatac taaatttcct
     4981 tattgaataa taattattcg tgttgtagca ttaaagaatg ctatgtttta atttagacaa
     5041 aaggtggtta ttgtgcccat aattttagat ccagaagtat taaaagtagc agagtatgtc
     5101 tatcaagaga gattatccaa accttacact gaagtaggcc cggaatggga gtacaatcat
     5161 aaaaaccctt atgccactcg cgctacaggc acagggcata atttgcaacg attcatcacg
     5221 atagatgatc agaaacttca tcgccccatt catggtttgg ctcataccat gcgcactctt
     5281 ttttacagtc aattaatgta tgaagcagcc aaaagacagc cacaccctca tcgatgtgct
     5341 gacggacgca ctatcgctga tctgagtgtg gaagacctta aaaagcttaa tatcgcacaa
     5401 ttattttttg tggctggacg agaaagcgag gcctcatatg gcgatgctta tcaccgatat
     5461 catctgtatg gcgctaaaca attcgaagaa tacgcccgta aacatttgac tcatctcttt
     5521 tcagaaaagg aaattgtcct ttattctcga tgcatcgaag atcgaatagg cgatcggttt
     5581 gatgaaaccg ctgaaggcta tctcattcac ttatcgcaca tgatcgattt aatgcgctgt
     5641 aaaagcccag tagaagtgtt tataggccat tcacgcggtg tttcaggtat cgtaccaacc
     5701 ttgattcaac tatttggtcg tgaagacggt ttagctatta tgcattatgc acgcagcctt
     5761 tttgctgcca ctggcgaggc tgttccttat atcagttcat cggaatggcc ccatttgggc
     5821 attgagtctg atagagtgga aagagcgctt aagattgtag gacctcttga agtagaaggt
     5881 caggaagccg atgctaaaaa aaccgcacaa gctggatttt cggtggatgg ctgctatggc
     5941 gctttggtaa aagtagacac acccgattgg tatcatcagg ttaaagaaaa agaagactat
     6001 gatgtcgatg aagtgatcgc tttgccaccg caaatcacta taagagaaga ggctcccaag
     6061 gcaaatcagc ctttcctgtt atcactcctt agacacgtat tttggtgttg tccttcaact
     6121 caaaaaagag acgatgaaaa tacagatgtc ttggaaaaaa aggcacaact atgaagaggt
     6181 tatgaggcag attattttat aattttcgag gtcatgagga cgcagtatgt ttggtttcat
     6241 aaagaaagta cttgaattgt ttggtgttga tcaatctgaa gataatccat ctgagactgt
     6301 ggtggaaaca accgatgttt caacaaaaat caaaaccact gacaccaccc aggaagagag
     6361 ttcagtcaaa accaaaacgg ttgttcctac ccaacccggg agttctgtca aaccagagac
     6421 aatcgcacct gaccaacaaa aaaaacacca aataaaaacc gaaacgacta caggcacgac
     6481 cacacaaaaa agccctaaag taacactaat ggatggtcac gtgaaacaat actattttgc
     6541 acggcggggt gaaacaagca ctcatgacac ctctctgcca ccacctgtga aggtgttaag
     6601 tgggcgctct atcccattaa aagaaattcc ttttgaagcc accagaaatg aattagtcca
     6661 gatttatctc acttccattg atcaactcat caaaagcaac aaattaggct ctgttccatc
     6721 acaacaactt gcctctcact acctctttct aagatcgctt gccaactcag aaactgatgg
     6781 gattaaaaaa aatcaaatac tgtctttagc caagccattg ggcgcttacc ttgcatctaa
     6841 agagccccat gtatggaaaa tgatcaatga actcattgaa aaaagtgaat atcccatcat
     6901 tcattactta aaaaataacc gtgctcattc caatttcatg ctggcattaa ttcatgagca
     6961 tcataaggga tcattaacaa aaaaccaaag cgcctttata caaaaattca gggattcctc
     7021 tgtctttctc ttccctaatc caatttatac agcatggctt gctcattcct atgatgaaga
     7081 ttccagcttt aatcctatgt tccgtgaaag attatccacc aatttttatc attccactct
     7141 gactgataat ctcttgttac gaacagaacc taaagaagta acgctttcat cagagcatca
     7201 ttatagaaaa gaaaagggac cgattgattc ttctttgcgt tatcaaatgt caagcgatcg
     7261 gttattgcga atccagggca gaactttact gtttagtacc ccccaaaatg atgtggttgc
     7321 agtcaaagta caaaaaaaag gcgagcctaa gtccaccttg gaagaagaat atcaaatggc
     7381 agattattta cttaaacatc aacatcgttt ggatttacaa agcgaattac cacagccctt
     7441 gggacaatac tccgttaaaa aatcagagat tttggaaata agcaggctat caccaaattc
     7501 tgagcgtttt agagatttaa tagacgacgc caaagacctt gaagtgtatg tttataaagc
     7561 tcccctctcc tatttcacct acctgcatga tgaacatcag aacttggaac agctgacaac
     7621 ttctgttaaa acaaatgtgc atgatctctt tgttctatta cgcgaaggca ttgtttttcc
     7681 ccaattggca gatatattcc atactcatat tggtgaagac gaacgagaag ataaaggaag
     7741 atatcaatcc ttggttcaac ttttaaatgt cttacagttc caattaggac gcatagataa
     7801 atggcaaaaa gccgtcgaat tcgtcaattt gcgcggcagt ggcctggctg atttgggaga
     7861 cagtctgccc ataacaagcc tgttcacttc ttctgatttc acaaaacatt acttctcgga
     7921 gctgcttaca ggaggttatc atcctacctt ctttgacaaa tcatctggca cagccaactc
     7981 tttattcacc ggcaaacgcc gattatttgg taattattta tatcttaata ccatagcaga
     8041 atatttgctg gttattcagt taacgcttgg cagctatggg gataaagtca ctcgagacat
     8101 gatggataaa cccaaaaaag aagctgtatg gcgagaattg gcaaacgtca tgttttcaag
     8161 ttgtgctgaa actattcata tcatgacagg aatacctcaa tcccgagcac taacactact
     8221 aaaacagcgc gctaatattg aaaaacattt cagacagaca caattctgga tgacgcctga
     8281 ttattccaaa ttggatgaag atacactgca aatggagcaa tacagcattt attccggtga
     8341 gccagaatat gaattcactg ataaattagt ctctggtgtt ggattgtccg tcgatggtgt
     8401 tcatcaggac ttgggtggat ataacaggga aagtccatta agagaacttg aaaaactact
     8461 ctatgccact gtgacactga ttgaaggaac catgcagctt gataaagaat tttttaaaca
     8521 attacaacaa gtcgaaaaaa tactttcagg tgagatcaaa acagatgcca gttcgtgctt
     8581 tgaagcagtt gcccaacttc tggaccttgc aaggcctggg tgccattttc agaaaagatt
     8641 ggttttgtcc tactacgaag aagccaagct aaaatatccc tccgcaccaa ctgactccta
     8701 tgattcacgt tttcaagtag tcgctaaaac caatgccgca ataaccattc aacgattctg
     8761 gcgagaagcg cgtaaaaact tgtcagaaaa ttcggatatc gattctgaaa aaccggaatc
     8821 cgaacgtacc tctgataaac gcttgcaata aaatggacag cctgcacctg actccttgaa
     8881 accacgccat ccccatggag aggtcgataa gacagcatag acttctccat ttttgacttg
     8941 taatttaaga aaaatatgag aaaaaccgcc cacttatgtt ccagccatca actcgatgaa
     9001 ttgcttgttt tttagccact taatacttt
//
LOCUS       DBNCWK010000124         8933 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.124, whole genome shotgun sequence.
ACCESSION   DBNCWK010000124 DBNCWK010000000
VERSION     DBNCWK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.124"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            183..2744
                     /locus_tag="AC06IO_002380"
     CDS             183..2744
                     /locus_tag="AC06IO_002380"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HIO9875552.1"
                     /translation="MQQVVIKFGGTSVSTRATWNNIAAITQKHLNTGVQPVIVCSALT
                     QISNKLEKAIEAALLDEHHSLFNDIQSSHLNLAEQLEVNHQLIANDLHQLQQWLTGIS
                     LLKQAPAKTHAQILSLGELMMTRLGHAFLEKQGIKVKWYDARELLTSTPTLGGETMNY
                     LSARCESEYDSALVEKFLSSGAQAIITQGFFAANPHGETVLLGRGGSDTSAALLAGKL
                     QAASCEIWTDVPGIYTANPHQLPHARLLKQLNYDEAQEIASMGAKVLHPNCIPPVRKA
                     NIPMVVKYTHLPEHSGTLITKDIDESAPLIKSIQVKHSILLISIDTLNMWQQVGFLAD
                     VFAAFKKHGFSVDLLSSSEFNVTLSLDVNAKIHDRPAINALLDDLNQFGRAKLIEPCS
                     AVSLVGHHIRTVLPHLGPALEVFEAKQVYLMSLASNDLNLTFVVDESHADKLCQRLHH
                     LLIESNPQIFYYSKSWHEEFGKPNVRPTPWWEIERDRLLTTSAIHSPCYVYHSPTQAT
                     RARQLSALESIDSLFYAIKANPFPTILKTLEKEGIGFECVSIQELELVLKLFPNIKKE
                     RILFTPNFAPKSEYEFALQTGCYVTIDSLYPLENWPDLFKDREVIVRIDPGTGAGHHK
                     HVSTGGNESKFGITQNDISKILSLTKVNHIKVIGLHAHSGSGILSTDLWQQTAVMLAS
                     LTDQFPEVRSINLGGGLGIVEKPGQHPIDFAALDAQLMAVKSQYPGLAIWLEPGRFFV
                     AESGVILAKVTQCKEKGKVKFIGIETGMNSLIRPSLYGAYHEIVNLTRLHEEKAGFAH
                     IVGPICESGDTLGYDRLLPVTREGDVILIANTGAYGHCMSSHYNLRPPAQEIVLE"
     gene            2749..5979
                     /locus_tag="AC06IO_002381"
     CDS             2749..5979
                     /locus_tag="AC06IO_002381"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIO9875553.1"
                     /translation="MRLIDSEQRSQATDPAESFIVQAPAGSGKTEILTQRFLRLLSTV
                     QSPEQIIALTFTRKAASEMRERIVQALHMAASNQPAVSAHQQSTLNFAKQALQRNNQY
                     QWDLLEQPNRLKIITIDSLCQSINRAIPLLEKQVAYPDISETPDSHYISAARCCIQYA
                     IATPEYQEAIKTLLLHVDNKQERLIQLFQALLSQRDQWLPTLFLAREQEKSTFEQALR
                     LIEQHELSRLRNSLPSDLAQELVLLSRELAVIENKPDSPRFILREWYEFQKCDQQIAG
                     ALSKLLLTSDNQLRKSFDHHVGLVKKNCAPNEFTRIKTASSDLFAKLQDCPDFLDALL
                     KVNQLPTPEYDKEQWDVLQALFLLLPLLVGHLQVSFGEHNEVDFTAISQQALAALGDL
                     DNPTDLALYLDHSIHHLLIDEFQDTSITQFELLTKLVQGWLPGEGKTLFLVGDPMQSI
                     YRFRQAEVGLFFQAKEHGIGPVRLKSLELRCNFRSTETIVNWVNDHFSKIFSQQVDIE
                     SGAVSFHPSVHVIEKNESSTIQAVQFKNREQEARHLIEIINSELNRNPEQTIAVLVRT
                     RTQLPEIISLLRQNNIPYQGTDIDLLANLVHLRDVWSLAHALLFPGNRLSWLSILHSP
                     YVGLGLSDIHCIAQYNSRKSIYHNLLHLDKIQDISEEGRIRANFFIQIMNQALLCRSH
                     SRLSDWIADTLKNLHVEKILDKAQLNDLEQLWMLLDQYEKEGRIPDMNEFLNEFNKLY
                     AQQTTPSRLQIMTIHKSKGLEFDTVILPGLGSQANRGESPMLRWLKLPTQDHGNLLLV
                     SPIKSANQDNCPLYDYLDQLDEEKSAYEAQRLLYVAVTRAKSRLYLLDHSAKSYKSSF
                     RNLLKYQEFIADPDEAQHEEQEFPLPKLVKLPIEYYQKQIPSLVQSQESSCPEISSGI
                     PRLIGIVSHQLLQWICDNHPQTINHVPWNLVHNELKALGLNQEAQQTALSMIKIQISQ
                     MYENKIGLWILSRHENEQNEYELLVEYENKLVTRIIDRTFTENGTQWIIDFKTGKEDI
                     NTLQKHRQQLNEYGRYLSDCTKFPIRCGLYYLANGHWIDWQYKMSA"
     gene            6186..7259
                     /gene="apbC"
                     /locus_tag="AC06IO_002382"
     CDS             6186..7259
                     /gene="apbC"
                     /locus_tag="AC06IO_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIO9875554.1"
                     /translation="MTIEDTVIKLIGSLKDPLLDLTGKEMNLQYKITTSNQAMHVVLT
                     AGYPTSLLETSYKPIVQKIVQDEFPNYQVTISIQQFIKAHKTQLTGKALRGVKNTIAV
                     ASGKGGVGKSTVTVNLAAALAKLGARVGILDADIYGPSIPLMLGETKPVQVKDNCYIP
                     VEAHGMQAMSIGYLTDTNQALIWRGPMLAKSLIQMLDITLWNELDYLFIDLPPGTGDI
                     QLTLVQKIPLTSAIVVTTPQNVATLDAQKAITMFSRTGIDVLGIIENMSTHICSHCGH
                     QEAIFGRGGAAALCDAYQCTLLGQLPLDSHVRRHCDEGVPTATHSSNQLTDTFIKTAM
                     RTAIELSKKPINYADRFPKIVVE"
     gene            7375..7929
                     /locus_tag="AC06IO_002383"
     CDS             7375..7929
                     /locus_tag="AC06IO_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HIO9875555.1"
                     /translation="MIRLLIITISVLCSGLTIAKSSYQIDLILFAQPQNGVKSIKSDS
                     PVPLIPVSPNAIQLHSDNNKKHMPYTLLPRSQSKLSDQHYLLSRKSQFQILGHYSWRQ
                     PAKSDSLVALPKINHNGWQIQGTLKVRQSNYYLFDADLQCLSPGNQETSFTVSQKQRL
                     KGSTIYYLDHPQLGMLVKIHKIAS"
     gene            complement(7977..8867)
                     /locus_tag="AC06IO_002384"
     CDS             complement(7977..8867)
                     /locus_tag="AC06IO_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIO9875556.1"
                     /translation="MNLRDLHYFVILADVKHFGEAAKRCFVSQPTLSMQIKKLEEELG
                     VVLFERTNKQVLLTDQGSKLLDRTRKILILIDEMKELARQSEDPFTGELRLGVIPTVS
                     PYMLPLVMPELKNEYPRLKVWLIEDQTHRLITKLEQGELDVAIMALPIDKRFSCQILY
                     EEKFYFACANTHPLAQAKSVNINDLKNQPIMLLEEGHCLREQAMAVCQSAKADDIADF
                     TATSLETLRLMVQAGMGVTLLPALSTLTASTNHLKCIPFSEPAPSRILGLFWRAGTPR
                     QVCFNAIAELITKNVQSKLA"
BASE COUNT         2874 a         1838 c         1642 g         2579 t
ORIGIN      
        1 gtaaaatcaa aggcatatga taaattttta ttcgagccta cctttcccaa cccatgcttt
       61 gtagactcaa ctcgtgactt gggttgtctt gagaaaaatc ttttttaaat tggggcttaa
      121 tctacttgta caaatacttt agtccatgct aaggtgtatt aattttgagc ggaatactaa
      181 tcatgcaaca agtagtcatc aaatttggcg gcactagtgt ttcaaccaga gccacttgga
      241 ataatattgc tgcaattacc caaaaacatc tgaacacagg tgttcagccc gttatagtct
      301 gttctgcttt aactcaaatt tccaacaaac ttgaaaaagc catagaggca gcccttcttg
      361 acgagcatca tagccttttc aatgacattc agagcagtca cctgaatctg gcagaacaat
      421 tagaagtaaa ccatcaatta attgctaatg atttgcacca attacagcaa tggctaaccg
      481 ggatctcctt actcaaacaa gcacctgcaa aaacacatgc gcaaatttta agtttgggtg
      541 agctgatgat gacccgcctg ggccatgcct ttcttgaaaa acaagggatt aaagtaaaat
      601 ggtatgatgc cagagaattg ttaacaagca caccaaccct tggtggagaa acaatgaact
      661 atctctctgc ccgttgtgag agtgaatacg actcggcatt ggtagaaaaa tttctctcca
      721 gtggggctca agccattatt acccaaggtt tttttgctgc caatcctcat ggtgaaaccg
      781 ttttgcttgg tcgaggaggc tctgatactt cagcagcact acttgcggga aaactacagg
      841 ccgcttcttg tgaaatatgg actgatgtcc caggcattta taccgccaac ccgcatcaat
      901 taccccacgc ccgattatta aagcaattaa attatgatga agctcaggaa attgcctcaa
      961 tgggggccaa agttttacac cccaattgca taccacctgt tcgaaaagcc aatatcccta
     1021 tggtagtcaa atacactcac ctgcctgaac attcaggaac attgattacc aaagacattg
     1081 atgaatcggc acccttaata aaatccatcc aggttaagca cagcatactg ctaatttcta
     1141 tagacacttt gaatatgtgg caacaagtag gatttctggc tgatgtattt gccgccttta
     1201 aaaagcatgg gttttcagtt gatttgcttt cttcttctga attcaatgtc accctatccc
     1261 tggatgttaa tgccaaaata catgatagac cagcaattaa tgccttgctc gacgatttaa
     1321 accaatttgg acgcgccaaa ctgatagaac cctgtagtgc tgtcagtttg gtaggacatc
     1381 acatccgaac tgtattacct catcttggcc cggctttgga agtatttgag gcaaaacaag
     1441 tatacctgat gtctctggcc tcgaatgacc ttaatttgac ttttgttgtt gatgaatcac
     1501 atgccgataa attatgccaa agattgcatc atctactaat cgaaagtaat cctcaaatct
     1561 tttattactc caaaagctgg catgaagaat ttggcaaacc caatgttcgc ccaacacctt
     1621 ggtgggaaat tgaaagagac cgcttgctta caaccagcgc aatacattct ccttgttatg
     1681 tttaccatag tcccacccag gcaactcgtg caagacaatt atctgctctg gaatcaattg
     1741 atagcttgtt ttacgctatt aaagcgaatc cattcccaac cattttaaaa actctggaaa
     1801 aagaagggat aggttttgaa tgcgtttcta ttcaggaatt ggaattggtg ttaaaactgt
     1861 ttcccaatat taaaaaagaa agaattttat tcacacccaa tttcgctccc aaatccgagt
     1921 atgaatttgc tttgcaaacg ggctgttatg tgactattga tagcttatac cctttggaaa
     1981 attggcctga cttgtttaaa gaccgcgaag taatcgttcg catagatcct ggtacaggag
     2041 ctggccatca caagcacgtt tctactggtg gaaatgaatc caaatttggt ataacacaaa
     2101 atgacataag caagattcta tcacttacca aagtcaacca cattaaggtc ataggcttac
     2161 atgctcattc tggtagtggc atactctcaa cggatttatg gcaacaaact gccgtgatgc
     2221 ttgcctccct aaccgatcaa tttcctgaag tgcgttcaat taatttaggt ggcggtctag
     2281 gtattgtaga aaaaccgggc caacatccca ttgattttgc tgcgcttgac gcgcaattaa
     2341 tggcagtaaa atctcaatat ccaggattag caatctggct tgaaccaggt cgcttctttg
     2401 tagctgaaag tggtgtcatt cttgccaaag taacgcaatg caaagaaaaa ggaaaagtaa
     2461 aatttattgg tattgaaaca ggaatgaact cattgataag gccttcctta tatggtgcgt
     2521 atcatgaaat tgtcaattta actcgactac atgaagaaaa ggccgggttt gctcatatag
     2581 ttggcccaat ctgtgagtcg ggagatacat tgggttatga ccgcttatta ccagttacca
     2641 gagaaggcga tgtgatatta attgcaaaca ctggagccta tggtcactgt atgagttcac
     2701 actacaattt aagaccacct gctcaggaaa tagttctgga ataatggcat gcggttaatt
     2761 gatagtgaac aaaggagcca agcgacagat cctgctgaat catttatagt ccaggctcct
     2821 gccgggtcag gaaaaacaga gattcttacc caacgctttt tacgtttgct aagcactgtg
     2881 cagtctcctg aacaaattat cgccttgact tttacccgca aagcagcaag tgaaatgaga
     2941 gaacgaatcg ttcaggcatt acatatggcc gcgtcaaatc aaccggctgt cagcgctcat
     3001 caacaaagca ctttaaattt tgcaaaacag gctttacagc gaaataatca atatcagtgg
     3061 gatttacttg agcaaccaaa tcgcttaaaa atcataacca ttgattcatt gtgccaaagc
     3121 attaacaggg caattccttt attggaaaaa caggtggctt atccagacat ttccgagaca
     3181 cccgattctc attatattag tgctgcacgt tgttgtatcc agtatgctat tgctacgcca
     3241 gagtaccagg aagcaattaa aaccttgctt cttcatgttg ataataaaca ggaacgatta
     3301 attcaattat tccaggcact tcttagccaa agagatcaat ggttgccgac attatttctg
     3361 gcacgtgaac aagaaaagtc aacttttgag caagctttac gccttattga acagcatgaa
     3421 ctctctcgtc taagaaatag cctgccatcg gacttggctc aagaactagt gcttttgtcg
     3481 cgtgaattag ccgttataga aaacaaacct gattcaccac gatttatatt aagagaatgg
     3541 tatgagtttc aaaaatgcga tcagcaaatt gcaggtgctt taagcaaatt actcttaact
     3601 agtgacaatc aattgcgtaa atcgtttgat catcatgtgg gattagttaa aaaaaattgt
     3661 gcaccaaatg aatttaccag aataaaaaca gccagttcag atttgtttgc taaacttcaa
     3721 gattgtcctg atttcctgga cgcgctcctt aaggtcaatc aattaccaac tcctgaatac
     3781 gacaaagagc aatgggacgt tttgcaagca ctctttttgt tactgccatt attggttgga
     3841 catttacaag tttcatttgg tgaacataac gaagttgatt ttactgccat ctctcaacaa
     3901 gccttggcag ctttgggtga tttggataat ccgactgatt tggcactcta tctggatcat
     3961 tcaattcatc atcttctaat agatgagttt caagatacct ccattacaca atttgaatta
     4021 cttaccaagt tagtccaagg atggttacct ggagagggaa aaaccttatt ccttgtaggc
     4081 gatcctatgc aatccatcta ccgtttcaga caggctgaag ttggattgtt ttttcaggcg
     4141 aaagagcatg gcataggacc agttcgcctc aaatcattgg aactaagatg taatttccgt
     4201 tcgacagaaa caatagttaa ttgggtaaat gatcatttca gtaaaatatt ttctcaacaa
     4261 gttgatatcg aatcaggagc agtatctttc catccttctg ttcatgtcat cgagaagaat
     4321 gaatcaagca ctattcaggc cgtgcaattt aaaaatcgtg aacaggaagc aaggcattta
     4381 attgaaataa ttaatagtga attaaaccgc aaccctgaac aaactatcgc agttttagtg
     4441 aggacaagaa cgcaactccc tgaaatcatt agcctacttc gccaaaataa tatcccttat
     4501 cagggaacag acatcgattt actagccaat ttagtgcatt tacgtgacgt ctggtcactt
     4561 gctcatgccc tgcttttccc aggaaatcga ttgtcctggt tatcgatact ccacagccct
     4621 tatgttggat tgggattatc tgacatccac tgcatagccc aatataattc aagaaaatca
     4681 atttatcaca atttgctcca tttagataaa attcaggata taagtgaaga aggcaggatt
     4741 agagccaatt tttttattca aatcatgaac caggctttgt tatgtcgatc acatagtcgt
     4801 ctatctgatt ggatagcgga taccttaaaa aatcttcatg tagaaaagat ccttgataaa
     4861 gctcagttaa atgatctgga gcaattatgg atgttattag accaatatga aaaagaaggt
     4921 cgaattcctg atatgaacga atttttaaat gaattcaata aattgtatgc ccaacaaacc
     4981 accccttccc ggttacaaat catgactatt cataaatcca aaggactgga attcgatacg
     5041 gttattctac ctggccttgg gtcacaagca aatcgtggtg aatctcccat gttgcgctgg
     5101 cttaagctgc ctacccaaga ccatggcaac ttgttactgg tatcacccat aaaaagtgca
     5161 aaccaggata attgcccttt gtacgattat ctggatcaat tggatgaaga aaaatccgcc
     5221 tatgaggcgc aaagattact ttatgttgct gttacccggg caaaatcaag attatatttg
     5281 cttgatcatt cggcaaaatc ctataaatct tcctttcgaa acttattaaa ataccaggaa
     5341 ttcattgcag atcctgacga agcccaacat gaagaacaag aattccctct gccgaaatta
     5401 gtcaaactac ctatagagta ttatcaaaaa cagattccca gtttagttca atctcaagag
     5461 agttcatgcc cagagatatc ttcgggtatt cctcggctaa taggcattgt gtcccatcaa
     5521 ctattacaat ggatttgtga taaccatccc caaacaatta atcatgtgcc atggaacttg
     5581 gtacataatg aacttaaagc attaggatta aaccaggaag cccaacaaac tgctctatca
     5641 atgataaaaa tccaaatttc tcaaatgtat gaaaataaaa ttggcctctg gattttatcc
     5701 agacatgaaa atgaacaaaa tgaatatgag cttctggttg agtatgaaaa taaattggtt
     5761 acccgtatta tcgatcgtac ttttactgaa aatggcacac aatggatcat tgattttaaa
     5821 accgggaaag aagatataaa caccttacag aagcatcgcc aacaattaaa tgagtacggg
     5881 cgctatctgt cagattgcac aaaattccca attcgatgtg gattgtatta tcttgcaaat
     5941 ggacattgga ttgattggca gtataaaatg agcgcttaaa aacacagcag ctatgcaatt
     6001 gcccaataaa atcaaatgac attatcttaa gctattcgtc ttactcatca gaaaagctat
     6061 acccaaaata catttctctc aagtagatgc attcatcaac ccagcaaaaa cgaatagtaa
     6121 tggtactagt gattagccga gacctaagtt atcattacct atttattttc ataccaggct
     6181 aaaaaatgac tattgaagat acagtaatta agttaattgg ctcattaaag gatcctttgc
     6241 ttgatcttac tggcaaagaa atgaatctac agtacaaaat aacaactagc aaccaggcta
     6301 tgcatgttgt cttgactgcc ggatatccta cctctttgtt agaaacgagc tataaaccaa
     6361 tagtacagaa aatagttcaa gatgaattcc ccaattacca agttactatt tctattcagc
     6421 agttcataaa agcgcataaa actcagttga caggcaaagc cctgcgagga gtaaagaata
     6481 ctatcgcagt ggcctctggc aaaggagggg tcggaaagtc tactgtcaca gtaaatcttg
     6541 cagcagcatt ggcaaaactg ggtgctcgag tcggcatttt ggatgcggat atttatggac
     6601 caagcatacc attgatgctt ggtgaaacaa aacctgtaca agtaaaagat aattgctaca
     6661 taccggttga agctcatggc atgcaggcca tgtctattgg ctacttgacc gatacaaacc
     6721 aggcattaat ttggagaggt cctatgctcg ccaagtcttt aatccaaatg ttagacatca
     6781 ctctttggaa tgaattggat tatttattca tagatctacc acctggcact ggagatattc
     6841 aactcacttt ggtacaaaaa attcctttaa cctctgccat agtagttact actcctcaaa
     6901 atgtcgcaac tttagatgcc caaaaggcaa taaccatgtt ttccagaaca ggcattgacg
     6961 tacttggaat aatagaaaat atgtccaccc acatttgtag ccattgtggt catcaagaag
     7021 caatctttgg tagaggtggt gcagctgcgc tgtgtgacgc atatcaatgt acattattag
     7081 gtcaactgcc gctggacagc catgtacgta gacattgcga tgagggagtt cccacggcaa
     7141 cacattcatc aaatcaatta actgacacct ttattaaaac agcgatgcga actgcaattg
     7201 agctcagcaa gaaacccata aactatgctg acagatttcc aaagatagtt gtggaataga
     7261 agtaatagct tctctactat gtattattcc cttcagatga gtatataata cataaaaatt
     7321 gagtttgcca cctcaaagat aaccaagctc acaaaaaaca aaaataaaaa tgtcatgatt
     7381 agattattaa taataacaat cagtgtatta tgttcgggac ttacgatagc caaatcaagt
     7441 taccaaattg atttaatact ctttgcacaa ccacaaaacg gtgttaaaag cataaaatca
     7501 gattcgcctg ttcccctcat tcctgtcagt ccaaatgcta ttcaactgca ttcggataat
     7561 aataaaaaac atatgcctta cactcttcta ccgcgatcgc aatcaaaact aagtgatcaa
     7621 cattatcttt taagccgtaa atcccaattt caaatcctgg gtcattattc ctggagacaa
     7681 ccagcgaaaa gtgacagttt ggttgcgcta cctaaaatca accataatgg atggcaaatt
     7741 caaggtaccc tcaaagtgcg tcaaagtaat tactatctat ttgatgctga cctgcagtgt
     7801 ttgtctccag gtaatcagga gacttctttt actgtttcgc aaaaacaacg cttaaaagga
     7861 agcacaattt attatcttga tcatcctcag ttaggaatgc tagttaaaat tcataaaata
     7921 gcttcatgaa agctcatcca gctaagcagg aatttacgta taatttataa taatttctat
     7981 gctaatttgg attgaacatt ttttgttatt aattcagcaa ttgcattaaa acaaacttgt
     8041 cgtggcgttc ctgctcgcca aaataacccc aagatacgag agggtgccgg ctcagaaaaa
     8101 ggaatacatt ttaaatgatt ggttgaggca gttagtgtag acaaagcggg cagtaaagtg
     8161 acgcccattc cagcttgtac cattaatctc aaggtttcca aactggtagc agtaaaatct
     8221 gcgatgtcgt cagctttcgc tgattgacat acggccatgg cttgctccct caggcaatgc
     8281 ccttcttcta gcaacataat aggctgattt tttaagtcat tgatattaac tgatttggct
     8341 tgggccaaag gatgagtatt tgcgcaagca aaatagaact tttcttcata taaaatttga
     8401 catgaaaaac gcttatctat tggaagtgcc atgatagcaa catccagttc tccttgctcc
     8461 aatttagtaa ttaatctatg agtttgatcc tctattaacc aaactttcag gcgagggtac
     8521 tcatttttaa gttcgggcat taccaagggc aacatgtaag gtgatacggt aggtataacc
     8581 cccaaacgca attcacctgt aaatggatct tccgactgac gagcaagttc tttcatttca
     8641 tcaatcagga ttaatatttt tctagttcga tctaataatt tggagccttg atcagttagt
     8701 aaaacctgtt tgttggtacg ctcaaataat acaacaccta attcttcttc cagctttttt
     8761 atttgcatac tcaacgtggg ctgacttaca aaacacctct tagcggcttc accaaaatgc
     8821 tttacatcag ccaggatgac aaaataatgt aaatctctta aattcataat ttctcgataa
     8881 taaaaaatct attgtgacac aaattataaa tcggcagtat aaaaatcatt ttt
//
LOCUS       DBNCWK010000125         8867 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.125, whole genome shotgun sequence.
ACCESSION   DBNCWK010000125 DBNCWK010000000
VERSION     DBNCWK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.125"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            434..3586
                     /gene="putA"
                     /locus_tag="AC06IO_002385"
     CDS             434..3586
                     /gene="putA"
                     /locus_tag="AC06IO_002385"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HIO9875557.1"
                     /translation="MLEKQSIHLPEGLRAAINKAYRMDELSLITELSEQAALDPQQMM
                     AIKTSATKLVQSVRSERKKSTGIDSFLTEYALSSDEGIALMCLAEALLRVPDNATIDN
                     LIKDKLAGGDWGAHRGQSESFFVNATTWALMLTGKVLTPEKAENTLTKALLKLVNRSS
                     EAVVRKAVDKAMRIMSKQFVMGRTINEALARAKKKEDRGYRYSYDMLGEAALTSADAA
                     RYFEAYKEAIISIGEKADKHSDVYRRPGISIKLSALHPRYSEFQYERVIEELPPKLLA
                     LSRLAKDYDIALTIDAEESERLDLSLDVIEKVFTDESLQGWNGFGLAVQSYQKRAFYV
                     LDWVAALARSKQRRIMVRLIKGAYWDSEIKKTQMQGFSEYPVFTRKVFTDVSFQACAK
                     KILTMTDAIYPQFATHNAYSVAMILNLVGGYRDFEFQCLHGMGNELYEQIVPANCYGI
                     PCRIYAPVGSHEDLLPYLVRRLLENGANSSFVNRIVDDKAPISELVEDPVAKSKSLLD
                     KINKNIPLPEDIFLPVRKNSKGFDFTNRLERALLQQELAKIESKEWQASPMIAGRKLS
                     RDLLQTVMSPQQPAYAIGSVQQATLDDVEVALNQAKLAFDSWRKKPVEERASCLNRFA
                     DLLQANMAELMVLTCREAGKTWSDGIAEVREAIDFCRYYAKKAQELMSSPQRFSGYTG
                     ELNELSLHPRGTILCISPWNFPLAIFTGQVVAGLVTGNCVIAKPAEQTPLIAAYAVKL
                     MHQAGIPEGVIQLIPGPGETVGAALVADKRIKAVLFTGSTDTANLINRTLATRGGEII
                     PLIAETGGQNAMIVDSSALLEQVVVDAVTSAFGSAGQRCSALRVLYVQEEVYPRTVEL
                     LKGAMAELVVGDPQWLSTDVGPVIDKEALSILKNHVENMRKHHEILYQCTVDDEALSG
                     YFMPPTAIAIDSISALEKEVFGPILHVIQFKRKDLDKVINQINQTGYGLTLGIHSRIN
                     ETVDYIRQRVHAGNCYVNRNMIGAVVGLQPFGGEGLSGTGPKAGGPNYLIRLCHERTY
                     TVDTTAAGGNASLMSIPEEG"
     gene            complement(3780..5174)
                     /locus_tag="AC06IO_002386"
     CDS             complement(3780..5174)
                     /locus_tag="AC06IO_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIO9875558.1"
                     /translation="MNHSKKVLNVFSLVMINVIAVDSLRTLPISAKLGFSLVFYYIFA
                     ALTFFIPVALVAAELATAYPNTGGIYVWVREAFGRRAGFITIWLQWIYNVVWYPTMLA
                     FIAATLSYLIAPHLGNNKFYLLGTALSLFWVFTFLNCFGMKLSSIVSIIGASIGTLLP
                     MIVIIVLGAVWIFQDRPVAVNYPTTWLPDFSSLGNLSLFSAVLFGLIGMEMSAVHAEE
                     VKNPQRDYPKALFYSALLIISTLSLGSLAIVIVVPNDSLSVVSGLVDAYAIFFNSYNM
                     PWMTSVIAVLIILGGLSGVSAWIIGPTKGLLVSARDGSLPALFSRVNKYGSPVAILLT
                     QGIIFTVLSTVFILLDSINAAYWVLSDLSAQMALLVYIMMFAAAIKLRYSKPEQPRGY
                     TIPGGNLVMSLISGIGIICCIAAMIVGFIPPSQIPIKNVFLFECFLIGGLILFVFIPW
                     LFAKKHDEQLCSEE"
     gene            complement(5440..8115)
                     /gene="acnA"
                     /locus_tag="AC06IO_002387"
     CDS             complement(5440..8115)
                     /gene="acnA"
                     /locus_tag="AC06IO_002387"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIO9875559.1"
                     /translation="MKVGQDSLSTKSQLTVDGKTYNYYSLKEAENKHFKGINRLPYSL
                     KVLLENLLRFEDGNTVTTKDIKAIADWLHNKTSQHEIAFRPTRVLMQDFTGVPAVVDL
                     AAMRTAIVKMGGNADKISPLSPVDLVIDHSVMVDKFASADALEVNTKIEIERNQERYE
                     FLRWGQKAFSNFQVVPPGTGICHQVNLEYLGKTVWNSENNGQLYAYPDTLVGTDSHTT
                     MINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQM
                     LRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIKYLELTGR
                     DKHTIALVEAYAKAQGMWYDKDNEEPVFTDSLHLDLSSVEPSLAGPKRPQDKVNLSSL
                     PVEFNNFLIEVGKEKEKEKTFAVKNKDFQMKHGHVVIAAITSCTNTSNPSVLMAAGLV
                     AKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRHAGLQTYLDQLGFNLVGYGCTTCIGNS
                     GPLPDDISHCVAEHDLVVSSVLSGNRNFEGRVHPQVRANWLASPPLVVAYALCGTTCS
                     DLSREPIGQDKEGNDVYLKDIWPSNEEIAAEVAKVSGTMFRKEYAEVFKGDAHWQGIQ
                     TSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPIKQAYVLALFGDSITTDHISPAGS
                     IKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRGTFANIRIRNEMTPGQEGGVTR
                     YVPTGETMSIYDAAMRYQENQQDLVIVAGKEYGTGSSRDWAAKGTNLLGVKAVITESF
                     ERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISDKLTPGAMVPVTIERQDG
                     GVEKIETLCRIDTADELEYYKNGGILQYVLRKISS"
     gene            complement(8200..8826)
                     /locus_tag="AC06IO_002388"
     CDS             complement(8200..8826)
                     /locus_tag="AC06IO_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1689 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875560.1"
                     /translation="MYHYLFSCHKSQESIDGLIEQVKQLLNHVEMEQKAYFLNLLTAR
                     VTEFQNELKSETSNTINKQQILIQYERFAKTLLICIKQPERTSSAIHNYHKGFYYPVA
                     IHDKIKLDPTIENAAIATLGVSLALLLGSIPTFIFNPLFGVIMVSLAVTLLLPSGFYL
                     LIPDSPDTTSKKEEEKRIFMEGAKIINPDVRIEEFDEQPYLSASFIKA"
BASE COUNT         2732 a         1666 c         1812 g         2657 t
ORIGIN      
        1 atcatatcat gaggttaatt acaggctgaa aaagagaatt gagttaaata ttgtcagttg
       61 gttcattaat tgcgctatat gtgtatccaa aaaaacatct tattggttat aattagatct
      121 tgttggttaa atttaattcc gtttttctgg gttaaaaagc atgtttttac tttatcgaac
      181 agcattgtcg tcatataata tgccacaaaa aggggggtga tgcattgatt ttatatagcc
      241 caatttattg ctcgatacta tgatttatat ctgaaatatt gccttgtcgt aaagacgggc
      301 atggcaaata agccagtgta agatatcata accgaatttt tcattgttat aataatgaat
      361 ttaatttttg tcttatatgg taaaaattta ataactattt ttttcaatta ccactttagt
      421 cttggagaac attatgctgg aaaagcagtc cattcattta ccggaaggat taagagctgc
      481 tattaataaa gcttatcgca tggatgagct ttctttaatt acagagctta gtgaacaggc
      541 ggcacttgat cctcagcaaa tgatggcaat taaaaccagt gctacaaaat tggttcaatc
      601 agttcgtagt gagagaaaga aaagcaccgg aatagattct tttttaacgg agtatgcctt
      661 atcaagtgat gagggaattg ccttaatgtg tctggcagaa gctttattac gcgttcctga
      721 taatgccacc atagataact tgattaaaga taaattagct ggcggtgatt ggggggcaca
      781 tcgtggacag agtgaatcat tttttgtaaa tgctactact tgggctttaa tgctaactgg
      841 taaagtttta actcctgaaa aagctgaaaa tacattaacc aaagctttac taaaattggt
      901 taatcgaagt agtgaggctg ttgttagaaa agcggtggat aaagccatgc gaattatgag
      961 caaacaattt gtgatgggac gcaccattaa tgaagcgtta gctcgagcga aaaaaaagga
     1021 agacagggga tacagatatt cttacgatat gcttggtgaa gctgccttaa catctgcaga
     1081 tgctgcaaga tattttgaag cctacaagga agcaatcatt tcaattggag agaaagctga
     1141 taaacattca gatgtatata gacgccctgg tatttccatc aaactttctg ccttacatcc
     1201 acgatatagc gagtttcagt atgagcgtgt cattgaagaa ttacccccca aattattagc
     1261 cttgtctcgt ttagcaaaag attatgacat tgctttgaca attgacgccg aggagtcaga
     1321 gcgtttagat ctttcacttg atgtgataga gaaagtgttt actgatgaga gtttacaggg
     1381 atggaatggg ttcggtttgg ccgttcaatc ctatcaaaaa agagcttttt atgtcttgga
     1441 ttgggttgct gctttagcaa gaagtaagca gcggcgtatt atggtcagat tgattaaagg
     1501 agcctattgg gatagtgaaa tcaaaaaaac gcaaatgcaa ggcttttctg aatacccggt
     1561 ttttacccgt aaagttttta ctgatgtgtc atttcaggct tgcgcaaaaa aaatcctgac
     1621 tatgaccgat gccatttatc cacaatttgc cacacataat gcttattctg tagcaatgat
     1681 tcttaatttg gtaggaggtt atagagattt tgaatttcaa tgccttcatg gtatgggtaa
     1741 tgaactttat gaacagattg ttccagcaaa ttgttatggt attccatgcc gtatttatgc
     1801 accagttggt agccatgagg atttactacc gtatttagtt cgacgattac tggaaaatgg
     1861 cgcaaactca tcttttgtca atcgcatagt tgatgataag gcacctatta gcgagttagt
     1921 ggaggatccg gtagctaaat caaaatcgtt acttgataaa ataaataaaa atataccatt
     1981 gccagaggat atctttttac ccgtacgaaa aaattctaaa ggctttgact ttacaaatcg
     2041 acttgaacgc gcattattgc agcaggagct ggcaaaaatt gaatcaaaag aatggcaggc
     2101 aagtcctatg attgcaggcc gaaaattatc cagggactta ctgcaaacag ttatgtcccc
     2161 acagcagcct gcatatgcta ttggctcagt gcagcaagcg acacttgatg atgtagaggt
     2221 ggcattaaat caagcaaaat tggcttttga ctcatggagg aagaaaccgg tagaagaacg
     2281 cgcttcctgt ctaaaccgat ttgcagattt actacaggcc aatatggcag aactcatggt
     2341 attgacttgt cgggaagctg gtaaaacctg gagtgatgga attgctgaag taagagaggc
     2401 tattgatttt tgtcgatact atgccaaaaa agctcaagag cttatgagta gtcctcaacg
     2461 gtttagtgga tacacggggg aattaaatga attaagttta catcctcgcg ggacaatatt
     2521 gtgtattagt ccctggaatt ttcccttagc tatatttacg ggacaagtcg tagctggact
     2581 agttacaggc aattgtgtta ttgcgaaacc ggcagaacaa acgccactaa ttgcagccta
     2641 cgctgtgaaa ttaatgcatc aggccggcat ccctgaagga gtaattcaat taattccagg
     2701 ccctggcgag actgttggag ctgctttggt cgcagataaa cgaattaaag ccgtattatt
     2761 cacaggctca actgacacgg cgaatttgat taatcggacc ttagctactc gaggtggcga
     2821 aattattcct ttaattgcgg aaacgggcgg tcaaaatgca atgattgtcg attcttcagc
     2881 attattggag caagtggttg ttgatgctgt cacctctgcc tttggcagcg cgggacagcg
     2941 atgctcggca cttagggtat tgtatgtgca agaggaagtg tatccaagaa ctgtagagct
     3001 attaaaaggt gccatggctg agctagtcgt aggggatcct caatggttat caaccgatgt
     3061 tggccctgtt atagacaagg aagcattatc tatattgaaa aatcatgtcg aaaacatgag
     3121 aaaacatcat gaaatattat atcaatgcac tgttgacgat gaagctttat caggatattt
     3181 catgccgcca acagctattg caattgatag tatcagcgct ttggaaaaag aagtatttgg
     3241 gccaattttg catgtgattc agtttaagag aaaagatctg gataaggtaa ttaatcaaat
     3301 caatcaaact ggctatggtt taacactggg aatacacagc cgaattaatg aaacagttga
     3361 ctacattagg caaagagttc acgcagggaa ctgttatgta aatcgtaata tgattggcgc
     3421 agtagtaggc ctgcagcctt ttggtggtga agggttatct ggcacaggac ccaaggccgg
     3481 aggccctaac tatttgattc gactttgcca tgagcgtacc tatacagttg atacaactgc
     3541 tgcaggaggt aatgccagtt taatgtctat cccggaagaa ggttagcagt attagaaatt
     3601 cacttaagag aataatacaa tcttaagtgg atttattaca ggagatatgc tcctgtctgc
     3661 aacggcaata tttggtcgat cttgtatgag ggctgatcta ttacaattga ttaataactt
     3721 ttaagaatca tcttgaaaag cccaacaaaa ttttttgtgg gcttatattt cttgctcaat
     3781 tactcttcac tacataattg ctcgtcgtgt tttttagcaa ataaccaagg aatgaaaaca
     3841 aatagaataa ggccgccaat cagaaaacat tcaaagagaa aaacattctt tatagggatt
     3901 tgtgaagggg ggataaatcc gacaatcata gcagctatgc agcagatgat cccaattcca
     3961 gagataagag acattaccag atttccacca ggaatagtat agccacgcgg ctgttcgggc
     4021 ttgctgtatc gcaatttaat tgctgcagcg aacatcatga tatataccaa caatgccatt
     4081 tgagcactta aatcgcttaa tacccagtat gcagcattaa ttgaatcgag taaaatgaag
     4141 actgtactta atacagtaaa tataatgcct tgagtgagca gtatggctac cggcgagccg
     4201 tatttattga cacgggaaaa cagtgcaggt aaggaaccat cgcgtgcaga cactagtaat
     4261 cctttggttg gacctattat ccaggctgaa acgccactga gacctcctaa aataattaac
     4321 acggcaatca ctgatgtcat ccacggcata ttataggaat taaaaaatat ggcgtaggca
     4381 tcaacaaggc ctgaaacaac gctcaaacta tcgtttggta ctacaataac aattgccaaa
     4441 gaacctaatg ataaggtgga tataattaaa agagccgaat aaaaaagcgc ttttggataa
     4501 tctctttgcg ggttttttac ttcttcagca tgaacagcag acatttccat ccctataagg
     4561 ccaaataaaa ccgctgaaaa taaagacaga tttcctaaag aactaaaatc aggtaaccat
     4621 gttgtggggt aattgactgc tacaggtcta tcttgaaata tccaaactgc gcccaggaca
     4681 ataatcacta tcataggaag tagtgtgcct atggaggcgc cgataatact tacaatgctg
     4741 gatagtttca tgccaaagca attcaaaaaa gtaaacaccc aaaacaagct aagtgctgtg
     4801 cctaataaat aaaatttgtt attccccaaa tgaggagcga ttaaatagga taacgtggcg
     4861 gcgataaaag ctaacatagt tggataccaa accacgttat agatccattg cagccaaata
     4921 gtgataaatc ctgcccgtct cccgaaggct tccctgaccc agacataaat tccgccggta
     4981 ttaggatagg cggtagcgag ctctgcagca accaatgcta ctggaataaa aaaggtaagt
     5041 gctgcaaaaa tataataaaa gaccagagaa aatccaagtt ttgcgctgat aggcaaggtc
     5101 ctcaaactat ctacagcaat gacgttgatc ataactaaag aaaaaacgtt cagaaccttt
     5161 tttgaatgat tcattattta tcctttatga gtgcaataaa cccgggtatt gaatggggta
     5221 taaatggtat tatgataagt taacggagta aagaaaagtt tgtctgtaca gtttaattac
     5281 tgcttaatat ttataagttg ttgggtttct cctgattatg gtatcaattt ttactgatat
     5341 tctaatttca gctgcctagt gatgcagtgt tcatgcgtct ctagattatt ttgtgaaccc
     5401 aacactctaa gggagaaggg cacgcaatgt gcccaattgt taagagctta ttttgcgtaa
     5461 tacatactga agtattccgc catttttata atattcgagc tcgtcagcag tatcaattct
     5521 acaaagagtt tctatcttct caacgccccc atcttgcctt tctatggtta caggtaccat
     5581 cgcgcccggg gttaatttat cagagatttc aatactgatg cgttcgctac catccaattt
     5641 taatgtttta cgagtagtac cctctttaaa ttgcaatggc aatataccca tgccaattaa
     5701 attggaacga tggattcgct caaagctttc agtaatcact gctttaacac caagcaaatt
     5761 agtgcctttt gctgcccaat cgcgagaaga acctgttcca tattctttac cagcaacgat
     5821 aaccaagtct tgttgatttt cctgataacg catggctgca tcataaatcg acatggtttc
     5881 tccagtagga acataacgag tgacgccacc ttcttgtcca ggagtcattt cattacgaat
     5941 gcgtatattg gcaaaagtgc ctcgcatcat gacctcgtgg ttaccgcgac gtgatccata
     6001 ggagttaaaa tctttctcat ctaccccttt ggattttaaa tataagcctg ctggagaact
     6061 ggctttgatt gaacctgcag gtgaaatatg atccgttgta attgaatcac cgaataaggc
     6121 taaaacataa gcctgcttga taggtttgag cggttctggt ttcagactta aattttcaaa
     6181 aaatgggggg tgttgtatgt atgtagaatc aggattccat tcataagttt gtcctgagct
     6241 tgtttgaatg ccttgccaat gagcatctcc tttaaatact tcagcatact ctttacggaa
     6301 catggtacca cttactttag cgacttcagc agcaatctct tcattagatg gccagatatc
     6361 cttgagataa acatcgtttc cttctttgtc ttgcccaata ggttccctgc ttaaatcgct
     6421 gcaagttgta ccgcatagag cataagcgac tactaatggg ggagaagcca gccagttggc
     6481 tctgacttgc gggtgaacac gcccttcgaa atttctatta ccagacagta cagatgaaac
     6541 aacaagatca tgctctgcaa cacaatggga aatatcatca ggcaaaggac ccgaattacc
     6601 tatacaagta gtacaaccgt aaccaaccaa attaaaaccc agttgatcca ggtaggtttg
     6661 taggccggca tgccttaaat agtcagtaac taccttagaa ccaggtgcca atgaagattt
     6721 aacccagggt ttgcgttgta atcctttctc aatcgctttt tttgctacca gtcctgctgc
     6781 catcaataca cttggattag aggtgttagt acagctggtt atcgctgcaa tgactacatg
     6841 accatgtttc atttgaaaat ctttgttttt aacagcaaag gttttttctt tctctttttc
     6901 tttaccaact tcaattaaaa aattgttgaa ttctacaggt aatgaactga gattgacttt
     6961 atcttggggg cgtttggggc cagccagaga gggttcaacg ctgcttaaat ccagatgtaa
     7021 agagtcagta aacacaggtt cttcattgtc tttgtcatac cacatacctt gtgcttttgc
     7081 ataagcctca acaagggcaa tggtatgctt gtcacgacca gtcaattcca agtactttat
     7141 tgtttccttg tctaccggaa agaagccaca tgttgcgcca tactcgggag ccatattgga
     7201 aattgtagct ctgtctgcca aaggtaaatc attaagtcca ggaccataaa attcaacaaa
     7261 tttaccaaca accccttttt ttcgcagcat ttgagtgacg gtaaggacca aatcagttgc
     7321 agtaatgcct tctttaagtt tacctgataa tttaaaaccg attacttcgg ggattagcat
     7381 ggatacaggt tgccccaaca tggctgcttc cgcctctata ccaccgactc cccagcccag
     7441 aacacctagt ccattaatca tggtcgtgtg agaatctgtg ccgaccaaag tgtccggata
     7501 ggcatacaac tggccattat tttcactatt ccacacagtt ttccctaaat actccaggtt
     7561 gacctgatga caaattcctg ttccgggtgg gactacctga aagttggaaa aagctttttg
     7621 tccccagcgt aaaaactcat agcgttcctg atttctttct atctctattt tggtattcac
     7681 ttcaagggca tcagctgaag caaatttatc taccatgact gaatggtcta tgaccaaatc
     7741 gacaggagaa agaggagaaa ttttgtctgc gttacctccc atttttacta tggcagtacg
     7801 catggcagcc aaatctacta ctgcggggac accagtgaaa tcttgcatca ggactctagt
     7861 tggtctaaat gctatttcat gctgggatgt tttattatgg agccagtcgg caattgcctt
     7921 aatgtctttg gtcgttactg tattgccatc ctcaaaacgt aataaattct cgagaagtac
     7981 tttcaaggag taagggaggc gattaattcc tttaaaatgt ttattttcag cttctttcaa
     8041 actatagtaa ttataggttt ttccatcaac tgttaactgt gattttgttg atagactgtc
     8101 ttgacccact ttcataaagt actcccaaaa tcattattat taataatctc taggaataat
     8161 agctcaattt caggaagttt tcattcagta tcaataaaat catgctttga taaatgatgc
     8221 ggagaggtaa ggttgttcat caaactcttc gattctaaca tcaggattaa tgatttttgc
     8281 gccttccata aaaatccttt tttcttcttc ttttttgctt gttgtatctg gagaatctgg
     8341 gattagtaga taaaatccac ttggtaatag caatgttacc gccagtgata ccatgataac
     8401 accaaagagt gggttaaaaa tgaatgttgg tattgaacct aaaagcaaag caaggctaac
     8461 tccaagagta gctatagctg cattttctat agtgggatcg agtttaattt tatcgtgaat
     8521 agcaacagga taataaaatc ctttatggta attgtgtatt gcagaggatg ttcgttcagg
     8581 ttgctttatg caaatgagca aagttttcgc aaatctttca tactggataa gtatctgttg
     8641 tttattaata gtatttgagg tctctgattt aagttcattt tgaaattcgg taacacgggc
     8701 agtcaacaaa tttaaaaagt acgccttttg ttccatttcc acatgattta gtaattgttt
     8761 tacttgttca atcaagccat ctatagattc ctgagattta tggcagctga ataaatagtg
     8821 atacataata aatttctctc cctataaaat atgtcaaaaa actagca
//
LOCUS       DBNCWK010000126         8748 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.126, whole genome shotgun sequence.
ACCESSION   DBNCWK010000126 DBNCWK010000000
VERSION     DBNCWK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.126"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(155..991)
                     /locus_tag="AC06IO_002389"
     CDS             complement(155..991)
                     /locus_tag="AC06IO_002389"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIO9875561.1"
                     /translation="MANPYLSKLRVTFAGLLFYWFLPIRKQIVLNNIDIVFKNTATSA
                     EKIRLAKAFYSHVASTLKEMFLMNRPSSKKLNQQVEIHGVEHLQRAIDQKKGVLLLTG
                     HLGSWELISVLGFSRLISQYGQINVIRRPIRMKWLETWLFKRIAHHGLGVINSIGAPK
                     KINQAIKNKELVLFAMDQHAQLENKSGIAVEFFGVKAGTYRSLAFFAHKHQTPVIPVS
                     GYRQHNGKHVMKFYPEIEWEFHAVKEQAIYQNTLRYNQTLEKLILEHPEQWWWVHRRW
                     KL"
     gene            complement(984..1832)
                     /locus_tag="AC06IO_002390"
     CDS             complement(984..1832)
                     /locus_tag="AC06IO_002390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIO9875562.1"
                     /translation="MLELKRDLNTLPLTWWGRCAYFLSPYKRSNAKKNIDRIFQSFLS
                     PAERKRLCMAYYSHMITNIKEIILYALFSKKYLAKRIEIIGLEYLQDAISKGKGTLVF
                     TGHFGNWEFAPLFFLDKFDSKNHVFYCVRKNLRFAFLDSIFLRRFESCGFKIINAKNA
                     VSQVRTALKNNGIVFFPFDVHPSSKIKNRVTADFLGQETATNISLAYLATLYESCVLS
                     IAFYRTNKKQHVLCIYPEVKGVVNSEYKQALIENTQLYNKRLEEMLLLYPEQWLWSYK
                     RWKNNG"
     gene            1995..3152
                     /gene="lpxB"
                     /locus_tag="AC06IO_002391"
     CDS             1995..3152
                     /gene="lpxB"
                     /locus_tag="AC06IO_002391"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIO9875563.1"
                     /translation="MIKKRQTRIAMIAGEMSGDLLGAGVIRELKKHLKNVEFIGVGGP
                     QMLEEGFQSLANMSELSVMGISDVLRRYPQLYFIRERLLKEWTINPPDVFIGIDYPDF
                     NLSVETRLKRQNVKTVHLVSPKVWAWRQKRVYLIKKAVDLVLTLFPFEESFYQQYDVP
                     AQFVGHPLADLIEINPNNAELRKKYNYKPDDSILAVLPGSRIGEIKYMGPLFLEVMQR
                     IAVEMPHVHFIVPIACQELYPVFFKQFQARYRHLKIQIIQGNAREAMAISDVVLTKSG
                     TATLEAMLLKRPMVVAFKWSKFTHAIIAPQVKIPYVALPNLLANKKLVPEFVQEKATA
                     NSITESVLNLLACPSQSNLNKQFTAIHHTLRQNANEKAALSILKILETSSA"
     gene            complement(3124..4155)
                     /gene="lpxD"
                     /locus_tag="AC06IO_002392"
     CDS             complement(3124..4155)
                     /gene="lpxD"
                     /locus_tag="AC06IO_002392"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIO9875564.1"
                     /translation="MSNYQFTKPAGPFYLAELAEISGATLYEGKGEAFTVSGLAKLSE
                     ATTHNLVMLHQKKYLKELKNTAARACIIGPEYVKFAPDSMYLLVHPNPYKAFALIAQA
                     FYPSEKPAGFIASSAVIETSAVIGSSCYIAHGTYIGNNAKIGSGCQIGVNTYIGDGVT
                     IGDDCLIEDNVSIRHAVIGKHVVIYPGARIGQDGFGFASDASGHYKIPHAGGVIIGNH
                     VEIGANTCIDRGSLDNTVIEDWCRLDNLVQVGHNVKIGKGSIIVAQVGIAGSTELGEY
                     VTLAGQAGVIGHLKIGKGATVLASGKVYKNVKAGDRVGGHPAVSISDWQKQIRFLKTA
                     IKPKKSPKS"
     gene            complement(4171..5001)
                     /gene="lpxA"
                     /locus_tag="AC06IO_002393"
     CDS             complement(4171..5001)
                     /gene="lpxA"
                     /locus_tag="AC06IO_002393"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIO9875565.1"
                     /translation="MQVSPLDHSHVSSKVVSFSQLIHPTALISPYAKIGPNVSIGPYS
                     IIGDNVSIGQGTTIGSHVSIQGWTQIGEDNQIETGAIIGAVPQDLKFAGEKSTVFIGN
                     NNIIREYVTINRGTAGGGGETRVGNHNLIMTSVHVAHDVQMGNNNIIANAVAIGGHVV
                     IDDWVTIGALCGIHQFVQLGRMSMIGAQSKITKDVLPYTLVSGNPPKRFGINVERLRR
                     NGYSSSERIDIQRAYKILFQEGQTLTDTIEMLKKEFQKSMDVNYILKFLENSKRSFYR
                     "
     gene            complement(5335..6444)
                     /locus_tag="AC06IO_002394"
     CDS             complement(5335..6444)
                     /locus_tag="AC06IO_002394"
                     /inference="COORDINATES: protein motif:HMM:NF017003.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HIO9875566.1"
                     /translation="MYLNSRCKLYALGLFLASSSVSYAGVMGAVSEDKGFFFNVTPIY
                     GDVSDSAIDKFPFVETVNASNNIISHTSDIDSRWGYSLSLGYLFGPEKSHDVVLSYTN
                     LTNRGSTNVVNANPNDILINKVSQILKSEDSFVDPNQQVGGLDLYGPAFSSFSGSYEF
                     QTADLITHRYFKSTFINNVQFSRYYGVKATELKKGFYAQYVGMGISRLQQFPFTESLE
                     PVNDQINYEAKYYGIGPRIGMGADWSLTRYISLMGDVSASLLGGSYDTRFLETLVTPM
                     QIPGIRRTGSYSFSQNTDTSLWAAGVLGANLGIAANFDMKNDSRVGIAGGISSEQYWT
                     EATPDYFSSKTARNRVSLNQRFSVRNLFIKLSYVC"
     gene            6725..7804
                     /locus_tag="AC06IO_002395"
     CDS             6725..7804
                     /locus_tag="AC06IO_002395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIO9875567.1"
                     /translation="MKILVIQQKKIGDVLLSSVICNNLKKLIPNAEIHYLMNQEALPV
                     IEGNPNINQAITIRPEHRAGVRAFIKFALQIRRQRYDVVIDAYTKLESWLIVLLCGAK
                     RRISYKKKYRTFLYTDNIPRESVKVTQPGRMIDLKLLLIQPFLNNRLPERSPKIYLSE
                     HEKQNAVFLFKKHGIPSRKNLMLNIVGSCPLKTYPLEYMATLVNQIAGQVDVNILFNF
                     LPHQKDIAQQIYELCNDEAKKNIFFELSNYDLRTFILIMNQCDAIIGNEGGAIHIAKA
                     LNKPSFTIFSPWVCKHDWSTFEDDFYHAAVHLSDFKPDLYQNTPEHTYKDKTLELFKD
                     LKPELIWPKLSTYVAYHFGNMHNAD"
     gene            complement(7858..>8748)
                     /locus_tag="AC06IO_002396"
     CDS             complement(7858..>8748)
                     /locus_tag="AC06IO_002396"
                     /inference="COORDINATES: protein motif:HMM:NF016908.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIO9875568.1"
                     /translation="LFNILCICFSITIIILLIKSNKVLNKKISQLQQQTFQIQNSLLA
                     PIHENLVELLKVYSARDENGPLELVRHGKENDGGYVAAVKAFKQADVLLGYGIHKDNS
                     FEDQFSLIYNKPSYGFDCGIEQVASKSKLFTLVKECIVSDAFLYNPANTNHKVTSFVE
                     QLDNLNIKDKKLFIKMDIEGAEYDAFSGIMKHKDNITGIALEIHFNDARSTQNAITLL
                     EQLEKHFVLIHVHGNNYAAATGFSTTKSLGTIPNVIELSYINKALVKNYNLSENQSHP
                     LKIDQPNNPNRPDAAFTIIN"
BASE COUNT         2871 a         1704 c         1454 g         2719 t
ORIGIN      
        1 agaggatgaa ttaagaaaat atatttattt tctgaaattt tggccactca aaggttatcc
       61 attaaaaaaa gcgaaaacaa agttaaagac tgtattcaga gctttgctaa gaggcaatag
      121 ttcgctaaag taactaatta aatacgctga tggtctataa tttccatcgt ctatgaaccc
      181 accaccactg ctctggatgc tctaaaatta atttttcaag agtttgatta tagcgaagag
      241 tgttttgata gatagcctgt tctttgactg catgaaactc ccattctatc tctgggtaaa
      301 atttcataac gtgcttgccg ttgtgttgcc tgtagccgga taccggaata accggtgttt
      361 gatgtttatg ggcaaaaaag gccaaactcc ggtatgttcc ggccttaact ccaaagaact
      421 cgacagcgat ccctgattta ttttctagtt gggcatgttg atccatagca aaaagcacca
      481 actctttatt ctttatggct tggtttattt ttttcggagc accgatgcta ttgataacac
      541 ctaacccatg atgtgcaatt ctcttaaata gccaggtttc taaccatttc atgcgaatag
      601 ggcgcctgat gacatttatt tgtccgtatt gagatattaa gcgtgaaaaa cctaatactg
      661 atattaattc ccaactccct aaatgacctg ttaataaaag aacgcccttc ttctggtcaa
      721 ttgctctttg caaatgctct actccgtgga tttccacttg ttgattcaat tttttagaac
      781 ttggcctatt catcaaaaac atttctttta aagtagaggc tacatgggaa taaaaagcct
      841 ttgccaatct tattttctca gcagaagttg ctgtattttt aaaaacaata tctatgttat
      901 ttaatacaat ttgtttgcga ataggtaaaa accaataaaa aagaagacct gcaaaagtga
      961 ctcggagttt agataaataa ggattagcca ttatttttcc agcgtttgta tgaccataac
     1021 cattgttcag gataaagcaa taacatctct tccaatcttt tattataaag ttgagtgttt
     1081 tctataagag cttgtttata ttctgaatta actactcctt ttacttccgg ataaatacac
     1141 agtacatgtt gttttttatt tgttcgataa aaagcaatag ataacacgca tgactcataa
     1201 agggtagcca aataagccaa gctaatatta gtagccgttt cctgtcctaa aaaatcagca
     1261 gttactctat ttttaatttt agaagaggga tgaacatcaa aaggaaaaaa aacaatgcca
     1321 ttatttttca aggcagtacg aacctggctt actgcatttt tagcattaat tattttaaag
     1381 ccgcagcttt caaatcgacg caaaaaaata gaatccaaaa aagcaaaacg caaatttttg
     1441 cgcacacaat aaaaaacatg atttttgctg tcaaatttat caagaaaaaa caagggtgca
     1501 aattcccaat tcccgaaatg tccggtaaag actaaagttc cttttccctt gcttatggcg
     1561 tcctgtaagt attcaagacc gattatttca atgcgttttg ccaaatattt tttactgaac
     1621 agcgcatata aaataatttc tttaatattt gttatcatat gggaataata agccatacat
     1681 aagcgttttc tttcagccgg tgataggaag gattgaaata tacgatctat attttttttc
     1741 gcattgcttc ttttgtaagg cgataaaaaa taggcgcatc gcccccacca agtcaaagga
     1801 agagtattta aatcacgttt taattctaac ataaatgtaa actgccattt gttttgtcca
     1861 aataccgctc ccgcttacaa aagcggtttt aggcaatcag tctacaatcc aaccatttta
     1921 aagtaaaata ctacgtggat ttacaaacaa gtttaagcta acatccattc attcaattct
     1981 tttatgagca catattgatt aaaaaaagac agactcgaat tgccatgatt gccggagaaa
     2041 tgtctggtga tttgctaggg gcaggcgtaa ttcgtgaact caaaaaacat ttaaagaatg
     2101 tagagtttat aggagttgga ggccctcaaa tgcttgagga aggattccag tctcttgcga
     2161 atatgtccga gctttcagtt atggggataa gtgatgttct gagacgctat cctcaacttt
     2221 attttataag agaaaggcta ttgaaagagt ggaccattaa tccaccagat gtattcatag
     2281 gaattgatta ccctgatttt aatttatcag ttgaaacacg actaaaacgc caaaatgtta
     2341 aaacagtgca tctggtgagc cctaaagttt gggcatggcg acaaaagcga gtgtatttaa
     2401 taaaaaaagc agtcgatttg gtattaactt tatttccatt tgaagagtca ttttatcaac
     2461 aatatgatgt accagcccag tttgttggtc atcctttggc agatttgatt gaaataaacc
     2521 cgaataatgc cgaactgcgc aaaaaatata attataagcc tgatgacagt attttagccg
     2581 ttctaccagg cagtcgaatt ggggaaatca aatacatggg ccccttgttt ctggaggtga
     2641 tgcaaagaat tgctgttgag atgccacatg tgcattttat tgttcctatc gcatgtcagg
     2701 aattgtaccc tgtttttttt aaacaatttc aagccagata caggcactta aaaattcaga
     2761 tcattcaagg gaacgctcgt gaagctatgg ctatctctga cgttgtttta acaaaatctg
     2821 gaacggcaac actggaagct atgctattaa aacgacctat ggttgttgct tttaaatgga
     2881 gcaaattcac tcatgcgatt atcgcgccac aagtaaagat tccctatgtt gctttaccta
     2941 atttattagc caataaaaaa ttagtgcctg aatttgttca ggaaaaagca actgctaaca
     3001 gtattacaga atcagtgctc aatttgcttg cctgccccag tcaaagcaac ttaaataaac
     3061 aatttacggc cattcatcac acattaaggc aaaatgccaa tgaaaaagct gctttatcca
     3121 tcctcaagat tttggagact tcttcggctt gatcgctgtt tttaaaaatc ggatttgttt
     3181 ttgccaatca ctaattgata cagccgggtg gcctccgact ctgtctccag cttttacatt
     3241 tttatagact ttaccacttg ctaaaacagt ggcacctttg ccaatcttca agtgcccaat
     3301 caccccagct tgtccagcca gagtgacata ctccccaagc tctgtacttc cagcaattcc
     3361 aacttgagca actataatag accctttacc aatcttgaca ttatgcccaa cctgcacaag
     3421 attatccaaa cgacaccagt cttcaatcac agtattatcc agagaacctc gatcaataca
     3481 ggtattagcg ccaatttcaa catgattgcc aataatgacc cctcctgcat gaggaatttt
     3541 ataatgacca ctcgcatcac tggcaaatcc aaaaccatcc tggcctatac gggcaccagg
     3601 ataaataacc acatgcttcc ctataacagc atgcctgatg ctgacattgt cttctataag
     3661 gcaatcgtcg cctatggtta caccatcacc aatataagta ttcactccaa tctggcatcc
     3721 ggaaccaatt ttcgcgttat tgccaatata agttccgtgc gcaatataac agcttgagcc
     3781 aattaccgca gaggtttcga ttactgctga ggaagcaata aagccagctg gtttctctga
     3841 aggataaaaa gcttgagcaa ttaaagcaaa agctttatac gggtttgggt gtaccagtaa
     3901 atacatgcta tcaggcgcaa atttcacata ctctggcccg ataatacaag cacgagcggc
     3961 tgtattcttt aattctttca ggtatttttt ttgatgcaac attaccaaat tatgggttgt
     4021 cgcttccgaa agttttgcca aaccagaaac tgtaaatgct tctcctttac cttcatacag
     4081 ggtagcgcca gaaatctcgg ctaattccgc caaataaaat ggtcctgcag gttttgtaaa
     4141 ttggtaattt gacatacaca ctctaaaaaa ttaacgataa aagctacgtt tagaattttc
     4201 aaggaatttg agaatataat ttacatccat acttttttga aactcttttt taagcatttc
     4261 aatagtatca gttaacgttt gaccttcctg aaagagaatt ttataagccc tctgaatatc
     4321 tattctttcc gaagaactgt atccatttcg acgcaatctc tcaacattga tgccaaatcg
     4381 tttgggagga tttccagaaa ccaaagtata gggcaaaaca tcttttgtaa ttttgctttg
     4441 tgcaccaatc atactcattc tgcctaactg aacaaattga tgaataccgc acaaagcgcc
     4501 aatagtcacc caatcatcaa taactacatg tccgccaatg gcaaccgcat tagcgataat
     4561 gttattgttt cccatttgaa catcgtgggc tacatggact gaagtcatga ttaaattatg
     4621 attaccgaca cgcgtttctc ctcctccgcc agcagttcct cggttaatag tcacatattc
     4681 tcgaataata ttattgttac caataaaaac cgtacttttt tcacctgcaa atttcagatc
     4741 ctggggaaca gcgccaataa ttgccccggt ttcgatttga ttatcctcac ctatttgtgt
     4801 ccatccttga atagaaacat gggagcctat tgttgttccc tgaccaatgc tgacattatc
     4861 cccaataatg gaataaggac ctatgctaac attaggtcca atttttgcgt aaggagatat
     4921 aagcgctgta ggatgaatca attgtgaaaa tgaaacaact ttactagaca catgactatg
     4981 atctaacgga ctcacttgca tgagggtacc tccaaaaaaa caaatacaaa aattagactt
     5041 tattaacaat ctaaacaacc gaagatttct gaaattccat attactaatc aaacttggct
     5101 acagtattca tgacattcga agaatccgta ctgatttcta ctaattctga ggggttttct
     5161 tgcaaactcc tggcatgaca gcctacagaa aaatatcatt cgaatatttc cagggaccaa
     5221 cagaataaca aatcgaaatt atagagtgct aataaatcct aatattattc actcatcaga
     5281 aaaacatttt ctgatgagtg aataataaaa ttttgaaaaa aatgtaacct acaattaaca
     5341 gacataactc aatttaataa atagatttct cactgaaaaa cgttgattta gtgaaactct
     5401 atttcttgct gttttgctgg aaaaataatc cggagttgcc tcagtccaat attgctcgga
     5461 gctaataccg cccgcaatac ctacccggct gtcattcttc atatcaaaat ttgcagcaat
     5521 gcctaaatta gctcccaata cccccgcagc ccagagtgaa gtatcggtat tttgactaaa
     5581 gctgtaactt cctgtccgtc ttatgcccgg gatctgcatg ggagtaacca gagtttcaag
     5641 gaatctggtg tcataagagc ctcccaataa agaagcagac acatccccca ttaaactgat
     5701 ataacgagtt aaggaccagt cagctcccat tccaatgcgc gggcctatgc cataatattt
     5761 ggcctcataa ttaatttgat cattaacagg ctctaggctt tctgtaaacg ggaattgttg
     5821 taaacgagat atccccattc caacatactg ggcataaaat ccctttttaa gttctgtggc
     5881 tttaactcca taatatcgag aaaattgcac attatttata aaagtacttt tgaaatagcg
     5941 atgtgtgatt aaatcagcgg tttgaaattc gtaacttcca ctaaaggaag aaaatgctgg
     6001 gccatagaga tccaaaccac ccacttgctg attcggatca acaaaactat cctcactttt
     6061 caggatttgg cttactttgt taattaggat gtcattaggg ttcgcattaa caacattcgt
     6121 tgaacctctg ttagtcaagt tcgtatagct taataccaca tcgtggctct tttccgggcc
     6181 aaacaggtat cccaaactta aagaatatcc ccagcgggaa tcgatgtctg aggtatggct
     6241 tataatgtta tttgatgcat tgacagtttc aacaaaagga aatttatcaa tagctgaatc
     6301 acttacatca ccataaattg gtgttacatt aaaaaagaaa cctttgtctt cactgacagc
     6361 ccccataact ccagcatagg aaacagaaga acttgccaaa aacaaaccca aagcataaag
     6421 cttacagcgt gaatttaaat acatactgct tttactccct aaaaacttga tagatcctct
     6481 aaaaaaacac tatggtatat taacaggaaa attatatttg tccatttgtg actgtattca
     6541 tttctgaaac tgagtattcc ccaatactat ttttaattca atctttttta gctcgtcttt
     6601 caaaagatac ccattgtttt ggatatgaaa ggccattaat gacaacaaaa aattaatgtt
     6661 gtccaaaata gaatctccag acataataga atcccatcta gagattccag atttaataat
     6721 aaaaatgaaa atattggtca tacagcagaa aaaaattggt gatgttttac tcagtagtgt
     6781 tatttgtaac aatttaaaaa aattaatacc gaatgcagaa attcattatt tgatgaatca
     6841 agaggcttta cccgtaatag aaggaaatcc caatattaat caagcaatta caatccgccc
     6901 tgagcataga gcaggcgtac gggcttttat aaaatttgct ctgcaaataa gacgccaacg
     6961 atatgatgtt gtgattgatg cttataccaa actggaaagc tggttaattg ttttgctttg
     7021 cggagcaaaa cgccgcatct cctataaaaa aaaatatagg acatttcttt atacagataa
     7081 tattccgaga gaatccgtaa aagttactca accagggcga atgattgatt taaaacttct
     7141 tttaattcaa ccttttttaa ataacaggtt gcctgagcgc tcacccaaaa tatatctttc
     7201 tgagcatgaa aaacaaaatg ccgtattttt gtttaaaaaa catggtatcc catctagaaa
     7261 aaatctcatg ctgaatattg ttggaagttg cccattaaaa acttatcctc tggagtacat
     7321 ggcaacgctc gtaaatcaaa tagcaggcca ggtggatgtt aatattttat ttaatttttt
     7381 gcctcatcaa aaagatatag cgcaacaaat ttatgaatta tgtaatgatg aagcgaaaaa
     7441 aaatattttc tttgaattat cgaattatga cttaagaaca tttattttaa ttatgaatca
     7501 atgtgatgcc atcataggta atgaaggagg cgctatacat atagcaaaag ctttaaataa
     7561 gccttcattc acaatttttt ctccctgggt atgtaaacat gattggtcca ctttcgaaga
     7621 tgatttttat catgctgcag ttcatcttag cgactttaag cctgatttat accaaaatac
     7681 ccctgaacat acatacaagg ataaaacctt ggaacttttt aaagatctaa aaccagaact
     7741 aatctggccg aaattaagca cctatgtagc ttatcacttt ggtaatatgc ataatgcaga
     7801 ttagagagaa agaggctaaa ttattacaaa aaaactattc gctggaagag tacttttcta
     7861 gtttattata gtaaaggcag catctggcct attgggatta ttgggttgat caattttaag
     7921 gggatgtgat tggttttctg ataaattata attttttacc agtgctttgt tgatatagct
     7981 taactcaata acattaggta ttgtacctaa cgattttgtc gttgaaaaac ctgtagccgc
     8041 agcataatta ttgccatgaa cgtgaattaa tacaaagtgt ttctctaact gctctaatag
     8101 tgtgatggcg ttttgcgtgc ttcttgcatc attaaaatga atctctagcg caataccagt
     8161 aatattatcc ttatgcttca taatcccgga aaaagcatca tactctgcgc cttctatatc
     8221 catcttaata aatagttttt tatcctttat attcaagtta tctaactgct caacaaaaga
     8281 ggtgacttta tgattcgtat tagcaggatt atataagaag gcatcactca cgatgcactc
     8341 ttttaccaga gtaaataatt tacttttact ggctacctgt tcaataccac aatcaaaacc
     8401 ataagatggc ttgttataaa ttagtgagaa ttgatcttca aaactgttat ctttatgtat
     8461 accgtatcct aaaagcacat cagcttgttt aaaagctttt acagcggcta cataaccccc
     8521 atcattttct tttccatgac gaactaattc caaaggccca ttttcatcac gagcagaata
     8581 gacctttaaa agctcaacta gattttcatg aataggcgct aatagagagt tttgtatctg
     8641 aaaagtttgc tgctgcagtt gtgaaatttt tttatttaat actttatttg attttattaa
     8701 taaaataata atcgtaatac tgaaacagat acaaagtata ttgaaaag
//
LOCUS       DBNCWK010000127         8456 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.127, whole genome shotgun sequence.
ACCESSION   DBNCWK010000127 DBNCWK010000000
VERSION     DBNCWK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.127"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            64..1035
                     /locus_tag="AC06IO_002397"
     CDS             64..1035
                     /locus_tag="AC06IO_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HIO9875569.1"
                     /translation="MLKKTSLALLGLAASGYALAGTMGPVCTPGNVTVPCEAKRWDLG
                     VQALYLKPVYDADMAYRQVHVAPTRYDDDQGWDWGYRLEGSYHFNTGNDATLTWIHYS
                     DFANPSGLVGRIDIPPLPPALAFPRLFSAIYKNRFEQVNLVMGQHADFGLVKDMRFYG
                     GLQYADIEIDNTNYYFGAFPQLGGVNLFDNTDYKGVGPVVGIDYAYALTNALSVTANG
                     AASVLYGKGKYNIGYVALPTGSSGAVVRSIYANKRTIVPSLEAKLGLNYAYNMAQGVL
                     NLEGGYQVMNYFNALHTQFLQNMANPVTNSDYGLYGPYIGAKYIGNA"
     gene            1331..2923
                     /locus_tag="AC06IO_002398"
     CDS             1331..2923
                     /locus_tag="AC06IO_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HIO9875570.1"
                     /translation="MKFKKIILALACLSSPLYADQDQQLKSEIQRLQHQAEDLQAQLN
                     RLQKQLANHKSSQQKHEQQAATKPAEPQSKPTVKSGAAIEEKYHSSKVEVHAPDAHPE
                     SISFYPTALIADNRVVTYIAGTPVVSSPFLGDRPAFDGSDYIVNISSINRDVRLLQQR
                     RRLYRAYQKIGYPIPNMPIISLSGKTEPAATFNNPFRSTNTDGDITLGSSELDVAAAL
                     NENVEAYIAIAYDESPPAIGPRVNNSAFNLNMGFVNIGNLDKSPLYFTAGQVYVPFGR
                     YSSAMVSSPVTMNLARTKTRPVIFGYKSQGDTGPFGAVYGYRSDTTLGRSGVGGVNLG
                     YIFGFDNDINGEVGGGFISSIADAGGMQSTGANVGTTFGGFGSITNGNENVRKTKAAD
                     VHGHVGYDRYNLTLEWVGAVQSFRPQDLSFNGQGARPQAAQAELGMTFMAFNRPASIG
                     VGYQWTKEALALNLPKRRYVGVFNISIWKDTVESIEYRHDIDYGLTQFANGAAPQGFV
                     NLPTLGTGKSADTVSAQIGVYF"
     gene            3129..4634
                     /locus_tag="AC06IO_002399"
     CDS             3129..4634
                     /locus_tag="AC06IO_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIO9875571.1"
                     /translation="MHRDCVRQEGLHKLNNLFCKWAAVTRILQGIIFRSRLSVSVLED
                     SDSVAVKQSPPSVTCKIKLSKFMQTSYIFIFLTLFLLVGLQTSFAAKIVELNIKGPIG
                     PATADYLERGIKSAQDADLIVILIDTPGGLYDSTRNIIQLFLLSDVPIVTYVSPTGAR
                     AASAGTYLMYASTLAAMAPGTQMGAASPVSLGTGFSEGEKDEKKKSTMENKVTHDAVA
                     TIRSLAQLRGRDLDFAEKAVTEGKSITANEALSKGVVNYIAKNRDDLLSQINGIKVSQ
                     NNKTITINTENPDIQVINPDWRTRFLSVITNPTVAYLLLLLGIYGIFFELVNPGYVLP
                     GVVGAVSMLFALYALQLLPINYAGLGLIILGILFVIAEAFTPSFGALGVGGTVSFILG
                     SIMLMNTEHLAFQIAWSAIWAMAVLNILIFVLVLGMLIKSRNQKIRHGLETLVGAKGR
                     ALGDINLEGQAVIKGEIWNVHSSSPIAANKSVKVTRALGLLLEVEEDQSVY"
     gene            4677..5432
                     /locus_tag="AC06IO_002400"
     CDS             4677..5432
                     /locus_tag="AC06IO_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIO9875572.1"
                     /translation="MGPFLVILLVAIGLLLASMFKVFREYERGVVFMLGRFWRVKGPG
                     LIIIIPIIQQVVRVDLRTIVMDVPSQDVISRDNVSVRVNAVVYFRVVVPENAIIQVEN
                     YFEATSQLAQTTLRSVLGQHDLDDMLAEREQLNSDVQKILDAQTESWGIKVSNVEIKK
                     VDLDESMIRAIAKQAEAERDRRAKVIHAEGELQASEKLLQASQVLAQQPQAMQLRYLQ
                     TLATIAVNNNSTIIFPMPMEIGDILTSMAGKKK"
     gene            5671..6237
                     /gene="dcd"
                     /locus_tag="AC06IO_002401"
     CDS             5671..6237
                     /gene="dcd"
                     /locus_tag="AC06IO_002401"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIO9875573.1"
                     /translation="MSIKSDRWIEKMALEHGMISPFQAGQVRENQNGKIISYGVSSYG
                     YDVRCSNEFKIFTNINSAIVDPKAFDENSFVDVQSDVCIIPPNSFALARTVEYFRIPR
                     NILTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYAYEGVAQMLFL
                     EANEVCAVSYRDRNGKYQGQTGVTLPRT"
     gene            complement(6253..6564)
                     /locus_tag="AC06IO_002402"
     CDS             complement(6253..6564)
                     /locus_tag="AC06IO_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIO9875574.1"
                     /translation="MIKSELIEHIAARMTHLTEKQVADGINRILELMSEALIHGQRIE
                     IRGFGSFSLHYRPPRNAHNPKTGEKVVTEAKYSPHFKPGKELRERVNSSRAKFPRLDK
                     D"
     gene            complement(6817..7533)
                     /locus_tag="AC06IO_002403"
     CDS             complement(6817..7533)
                     /locus_tag="AC06IO_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIO9875575.1"
                     /translation="MLDFISSKTLFLCLILFLILFWLCFDKARKELRINRWQKKLNLK
                     HHAQIFHLLYGDVNGFIISQQARKNRDAIEYVYGEIEFLPFTALLSMVNIDHNTIFYD
                     LGSGTGKAVVACAMVYPVHKSIGIELFPNLHQCACERLQKLAAMEGYTESSKKVSFIL
                     GDFLTVDLSEATLIFINSSTLFGATWEALNTRLNSLPQLSTVITTSKTLSSSRFKLVT
                     RAKIQMSWGVVFAFIHKKEN"
     gene            complement(7538..8263)
                     /locus_tag="AC06IO_002404"
     CDS             complement(7538..8263)
                     /locus_tag="AC06IO_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875576.1"
                     /translation="MEHYQEDDLARIKELLRHTGEFIAYFEFVEAKMMEWREGIEQQA
                     ERVLHIAQSLQNELTSMQNLISQSGVTHFKISAEKALAQGETHLHSLERTCNQFTQIF
                     QQQQERLNALTENCIDRIEKHTGQATQTLTLQLSKYDVQHFHRIANESCDHVDRVAND
                     AINKSNKMLNMLQFKVGLFAMLITVLTAFVIVIYLNDEMPWEMHHQAMSERQAGKVLL
                     QAWPKLSQKEKAKILNEDGLKPG"
BASE COUNT         2492 a         1577 c         1822 g         2565 t
ORIGIN      
        1 taattaagtt ctttttgagc ccatcaccaa aatgatgggc tttttttttg aaatggagtc
       61 catatgttga aaaagacaag tttagcccta ttgggattgg ctgcaagtgg ttatgcattg
      121 gcaggaacta tgggacctgt ttgtacgcct ggtaatgtca ctgttccctg tgaagcaaaa
      181 cgttgggatt taggtgtgca ggcactctat ctaaaaccag tttatgatgc cgatatggct
      241 tatagacaag ttcacgtagc gcccacaaga tatgatgatg atcagggttg ggattgggga
      301 taccgattgg aaggttctta tcactttaat actggtaatg atgcgactct tacatggatt
      361 cactacagtg attttgcaaa tcctagtggt cttgtcgggc gaattgatat ccctccattg
      421 ccgcctgcat tagcgttccc acggttattt agtgcaatat acaaaaacag atttgagcaa
      481 gtgaacctgg ttatgggtca gcatgctgat tttggcctag tcaaagatat gcgtttctat
      541 ggtggtttac agtacgctga tattgaaatt gacaatacca actattattt tggtgcgttt
      601 cctcagttag gcggagttaa cctgttcgat aataccgatt acaaaggagt aggtcctgtt
      661 gtaggaatcg attatgctta tgcactaacc aatgcattga gtgtcacagc aaatggtgct
      721 gcttcagtct tatatggcaa gggtaagtat aacatcgggt atgttgcatt gcctacaggc
      781 tcttctggag cagtagtgcg atccatctat gcaaacaaaa gaacgatagt tcccagtctg
      841 gaagctaagc tcgggttaaa ctacgcttac aatatggctc aaggagtatt aaaccttgag
      901 ggtggctatc aggtaatgaa ctattttaat gccctacata cacaattcct gcaaaacatg
      961 gcaaacccag taaccaatag tgattatgga ctctatggac catatattgg tgcaaaatat
     1021 attggtaatg cctaaacgaa gtcctttact taaatgcgtt cgtcattgac gaacgcattg
     1081 ctttaaaata ctgcttaaaa atcttgaaaa aaatttttta acaaaaatat aatcacgtta
     1141 atccattcta agtctaacca actaaacccc taatttattg atgtataggc aataaactat
     1201 aaatgcatac aaataaatca atttatttta aattttagcg gagtgatcct atagccagcc
     1261 agtaattgcc gcgctataat ccggtggcta tccgtttagg atgtaaaaat aataaacagg
     1321 agtaccccgc atgaagttta aaaaaattat tttggctctc gcttgtttat catcgccatt
     1381 atatgcggat caagatcaac aactgaaaag tgaaattcaa cgattgcagc atcaagcaga
     1441 agatttacaa gcccaactca accgattaca aaaacaatta gcaaatcata aaagctctca
     1501 gcaaaagcac gagcaacagg ctgccacaaa gcctgcagaa ccacagagta agccgacggt
     1561 taaatcaggg gctgcaatag aagaaaaata tcactcatcc aaagtcgaag tccatgcgcc
     1621 tgatgcccac ccagagtcaa taagctttta tccaacagca ttgatagccg ataaccgagt
     1681 ggttacttat atagcaggga ctcctgtagt aagctcgcca ttccttggcg ataggcctgc
     1741 gtttgatggc tctgattata tagtaaacat ttccagtatt aatagggatg ttcgtttatt
     1801 gcagcagcga cgacgcttat acagagccta ccaaaaaata ggttacccta tccctaatat
     1861 gccaattatt tcattaagtg gtaaaacaga acctgctgcg acatttaata atccatttcg
     1921 atcaactaat acggatggtg atattacgct aggctcaagt gaactggacg ttgctgctgc
     1981 attgaatgaa aatgtcgaag cttatattgc aattgcctat gatgaaagcc ctcctgcaat
     2041 tggcccgcgg gttaataact cggcatttaa tttgaatatg ggatttgtca acattggtaa
     2101 cttggataaa agcccgcttt attttactgc agggcaagtc tatgttccat tcggccgtta
     2161 ttccagtgca atggtaagtt ctcctgtcac aatgaatttg gcaagaacca agacaaggcc
     2221 tgtcattttc ggttataagt ctcaaggtga taccggtcct ttcggtgctg tttatggcta
     2281 cagaagcgat actacattag gtcgctcagg agtcggaggc gttaatttag ggtatatttt
     2341 cggatttgat aatgacatca atggtgaggt agggggcggt ttcattagct ctattgcaga
     2401 tgccggcggt atgcaaagca caggcgccaa cgtaggaaca acgtttggtg gttttggctc
     2461 cataacaaac ggtaatgaaa acgttcgaaa aacaaaagca gctgatgttc acggtcacgt
     2521 agggtatgat cgttataatc tgactttgga atgggttggt gccgtacaga gttttagacc
     2581 ccaggacttg agttttaatg gccaaggcgc cagacctcag gctgcgcaag cagagctggg
     2641 tatgactttt atggctttta acagaccagc ctccattgga gttggctatc agtggacaaa
     2701 agaagcatta gcactgaatc tacctaaacg acgctatgtc ggagtgttta acatttccat
     2761 ttggaaagac acagtcgaga gtattgaata caggcatgat attgactatg gacttactca
     2821 gttcgctaat ggtgctgcac ctcaaggttt tgttaatctt ccaactttgg gtacaggaaa
     2881 atctgccgat actgtttcag cccaaattgg tgtttatttc taaattattt ccattcaaat
     2941 agcaggcttg taacaattca attggttaca agctgatgtg tttaggggct gcttacaccg
     3001 gatgcagggt aagtatcctg cccagttcaa ctcactttga taatactgaa ttgacgtgac
     3061 cagccccttt gtttttttca acctgtgcta tcttttattt tataactacc tcttaaaggg
     3121 atgtaaaaat gcatcgggat tgcgtcaggc aagaggggct gcacaaactc aataatcttt
     3181 tttgcaaatg ggctgcggtg acaagaattc tgcaaggaat aatattcaga agcagactat
     3241 ctgtgtcagt tcttgaagat tcagattctg tagcagtaaa acaatccccg ccatcggtaa
     3301 cgtgcaagat caagctcagt aaattcatgc aaacaagtta tatttttata ttccttaccc
     3361 tgttcctttt ggttggattg caaacttcct ttgcggcaaa aattgtagag ttgaatataa
     3421 aaggaccaat tggccctgcg acagcagatt atttggaaag aggtattaaa agcgctcaag
     3481 atgctgattt gatagtgatt ctaatcgata caccaggcgg tttatatgat tcaacacgaa
     3541 atatcattca gttattttta ctttctgatg tgcccatagt gacttatgta agtcctactg
     3601 gagcaagggc tgcaagtgca ggaacttatt taatgtacgc gagtacatta gcggcaatgg
     3661 cgcctggtac tcaaatgggg gctgcaagtc ctgtcagcct tggcacagga ttcagtgaag
     3721 gtgaaaaaga tgagaaaaaa aaatcaacca tggaaaataa ggtaactcat gatgctgtag
     3781 caacaataag gtcgcttgct caactgagag gacgcgatct tgattttgct gaaaaggcag
     3841 tgacagaagg caaatccata acagcgaatg aagccctcag caagggggtg gtcaattata
     3901 ttgcaaaaaa tagagatgat ttattgtctc aaatcaatgg aataaaagtc agtcaaaata
     3961 acaagacaat aactattaat accgaaaacc ctgacattca agtaatcaat ccagattgga
     4021 gaacccgctt tctatcagtc attaccaacc ctacagtggc ttatttatta ttgctacttg
     4081 gaatttatgg aattttcttt gaattggtta atccaggtta tgttttacct ggagtagttg
     4141 gcgcagtttc aatgcttttt gcgctgtatg cgctgcagct gttacctata aattatgcag
     4201 gtttgggctt gattatttta ggtattttat ttgtcatagc tgaggcattt accccaagtt
     4261 ttggagcgtt aggtgtagga ggaaccgttt cttttatttt aggttccatt atgctgatga
     4321 acacggaaca cctggctttt cagatagcgt ggtcagcaat ttgggctatg gcagttctga
     4381 atatattgat ttttgttttg gttctgggga tgttaattaa atctcgaaac caaaaaatca
     4441 gacatggcct ggaaacgttg gtgggtgcaa aaggcagggc gttaggagac attaatctgg
     4501 aaggtcaggc tgtaattaag ggagagattt ggaatgtcca ttcaagctcg ccaatcgcgg
     4561 caaataaaag tgttaaagtc acgcgagcat taggattatt gttggaagtg gaggaagatc
     4621 agtcggttta ttagttggtt attccatatt gaatgaaaag gattaaggag agtgagatgg
     4681 gaccatttct ggttatatta ctggtagcga taggcttgct actggcttca atgttcaagg
     4741 tatttcggga atatgaacgt ggggtggttt ttatgctggg tcggttttgg cgtgttaaag
     4801 gtccgggatt gataatcatt atcccaatta ttcaacaagt ggtgcgtgtt gatttgcgaa
     4861 ccatagtcat ggatgtaccc agtcaagatg ttatctcaag agataacgtt tctgtacgcg
     4921 tcaatgccgt ggtgtatttt cgtgttgtcg ttcctgaaaa tgcaatcatc caggtagaaa
     4981 attattttga agcaaccagc caactggcgc aaaccacatt acgttcagta ttaggtcaac
     5041 atgatttgga tgatatgttg gctgaacgtg agcaactgaa tagtgatgtg caaaaaattc
     5101 ttgatgccca aacagaaagc tggggaatca aagtttcaaa tgttgaaatc aagaaggtgg
     5161 atttggatga aagtatgata agggcaatag ccaaacaggc tgaagcggaa agagacaggc
     5221 gagcgaaagt aatccatgcg gagggagagc tgcaagcttc ggaaaagtta ttacaagcct
     5281 ctcaagtact agcccagcaa ccccaggcaa tgcagctaag atatttgcaa actttagcca
     5341 ctatagcggt taataacaat tcaacgataa ttttccctat gccaatggag ataggtgata
     5401 tattaacttc catggccggc aagaaaaaat agaccttgaa cagtggcata agccccaaat
     5461 atacctaagt gtatagagtg ccgccattgg ataggagaac atggtgatga gattttttat
     5521 tccacaacag gttgtggtat cagtattttt ttaaaatgag agcttttatc gaagtttcaa
     5581 tcatcttatt ctgtataatt tcccaaaaca aactttcgtt tcattgactt ttatgataga
     5641 aatctatctt tttaagaaaa gaggaatatc gtgtcaatca aatcagacag atggatagag
     5701 aaaatggccc ttgagcatgg gatgatttct ccatttcagg ccggtcaagt gcgagaaaac
     5761 caaaatggta agattatttc ttatggcgtc tccagttatg ggtatgatgt tcgctgttcg
     5821 aatgaattca agatttttac gaatattaat tcagcgattg ttgatcccaa ggcgtttgat
     5881 gaaaatagct ttgtagatgt tcagtctgat gtctgcatta ttccccccaa ttcgtttgcc
     5941 ttggcaagaa ctgtcgaata tttccgtata ccccgcaata tattaacgat atgcttaggt
     6001 aaatccactt acgcgcgttg cgggattata gtgaatgtta ctcctctgga accggaatgg
     6061 gaggggcatg ttactctcga gttctccaat acgaccactt tgccagccaa gatttatgcg
     6121 tatgagggag ttgctcaaat gctgttttta gaggctaatg aggtttgtgc tgtttcttat
     6181 cgtgatcgaa acggaaagta ccagggacaa accggggtta cactaccacg cacttaggat
     6241 gaaaaataaa atttaatctt tatccaaacg tgggaacttg gctcgcgacg aattgacccg
     6301 ctctcgtaat tcctttccag gtttaaagtg aggactgtat tttgcctctg ttactacttt
     6361 ttcacccgtt ttaggattat gagcatttcg cggtggtcgg taatgaagag aaaaactgcc
     6421 aaagccacga atttctattc tttggccatg gataagtgct tcactcatta attcaagtat
     6481 tctgttgatg ccatcggcta cttgcttttc agtaagatgc gtcattcgag cagcgatgtg
     6541 ttcaatgagt tcggatttaa tcatatactc acctcgtttt gtcgcgtatt taatagaccg
     6601 attgcattct ctattatttt agcttgtctg cctatgcttc tattcgcttt gtatcttgaa
     6661 acacgggtgg ccgatactaa aataaactcc acgagctgag caactggtct aatgtctgct
     6721 accaagacac atcaggtgct gacaatttcc catttgattt tataagtata gttcgggtaa
     6781 aaaattattc aatgttttca agctggttag taaaaattag ttttctttct tatgaatgaa
     6841 tgcgaaaacc acaccccaac tcatttgtat tttagcgcga gtaactaatt taaaacgact
     6901 ggatgatagg gttttgctag tagtaatgac ggttgaaagt tgtggcaggc tattgagacg
     6961 agtgttaagg gcttcccagg ttgcaccgaa taaagtactt gaattaataa atattaaggt
     7021 cgcctcagat aaatcaacgg ttagaaaatc acccaatata aaagagactt ttttgctact
     7081 ctctgtatag ccttccatag ccgccaattt ctggagacgc tcgcaagcac attgatgtaa
     7141 gttagggaac aattcgatgc ctatactttt atggactgga tataccatcg cgcaggcgac
     7201 aacagcctta ccagttccag agccaagatc ataaaaaata gtattatgat caatattgac
     7261 cattgacagt aaagcggtga acggtaaaaa ctcaatttcc ccatacacat attctattgc
     7321 atctcgattc tttcttgctt gttgggaaat aatgaagcca ttaacatcac cgtacaggag
     7381 atgaaaaatt tgagcatgat gctttaaatt tagttttttt tgccatcggt taatgcgcaa
     7441 ctcttttctt gccttatcaa aacaaagcca aaaaagaatg agaaacagaa tgaggcataa
     7501 aaaaagcgtt tttgacgata tgaaatcaag cacagtatta tcctggtttc agaccatctt
     7561 cattcaagat ttttgccttt tccttttgac ttaattttgg ccatgcttga agaagaactt
     7621 taccagcctg gcgttcgctc atggcttggt gatgcatttc ccaaggcatt tcatcattaa
     7681 ggtaaatgac tatcacgaaa gcagtgagta cggtgattaa catggcaaaa agacctactt
     7741 tgaattgaag catattaagc attttattgc ttttgtttat ggcatcgttt gcaactcggt
     7801 cgacatgatc acagctttcg ttagcgatgc gatgaaagtg ttgaacatcg tatttagata
     7861 gttgcaaagt cagtgtttga gttgcttgac cagtatgctt ttcgattcta tcaatgcagt
     7921 tttctgttag ggcattaagc ctttcttgtt gttgttggaa gatttgggta aattgattgc
     7981 aagttctttc taaagaatgc aaatgcgttt cgccttgagc cagggccttt tcagcactta
     8041 ttttaaaatg ggttactcct gattgagaaa taaggttttg cattgaagtt aattcatttt
     8101 gtagtgattg agcgatatgt agaactcgtt cagcctgttg ctcaattccc tctcgccatt
     8161 ccatcatttt cgcttcgaca aactcaaaat aggcaataaa ttctccagta tgtctcagca
     8221 actctttaat gcgagccaag tcatcttcct gataatgctc catgcctctc tccttactaa
     8281 attcatagtc agagaatctt caaatagact tcttgcataa tctgtccttt tcattttatt
     8341 gccgtgttgc aaacgtttct atgtttgcac tttgcctaaa acgaaataat cagtttatac
     8401 aagagggcta ataatatcct ttgatgcatc ctgcctcttt ggccgtgaat ttagaa
//
LOCUS       DBNCWK010000128         8447 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.128, whole genome shotgun sequence.
ACCESSION   DBNCWK010000128 DBNCWK010000000
VERSION     DBNCWK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.128"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            30..254
                     /locus_tag="AC06IO_002405"
     CDS             30..254
                     /locus_tag="AC06IO_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875577.1"
                     /translation="MSKFLVFIGSILFFSSVLAGDIPEVDIQDQQSDQELCTQKFYNQ
                     CINKCEKTNYGDCTQACEENAKNQCLYAGE"
     gene            384..956
                     /locus_tag="AC06IO_002406"
     CDS             384..956
                     /locus_tag="AC06IO_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HIO9875578.1"
                     /translation="MLHNTKINKLAVFCGSNTGYSEVYKQSAENMADVLSSNGITLVY
                     GGSKSGLMGIIANRMLKNGSNVIGVIPKSLVDTEKAHDGLTELHVVNSMYERKVLMCK
                     LSDGFILLPGGSGSLDEFFEMFTLVQLGYHKKPCCVLNASGYYDYLLQFLDYAVNQGF
                     LHSKDRNALIVNQYPDTLIENLFEVINNRK"
     gene            996..1733
                     /locus_tag="AC06IO_002407"
     CDS             996..1733
                     /locus_tag="AC06IO_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIO9875579.1"
                     /translation="MPKKLLVAAFLCNIFCSPSHLYASSTEIPILGKTMTQAKQQQMT
                     PKQALQRLKDGNQRFLSNKPLARDYLKQAKQSAYGQYPFAVILNCMDSRSVPEFFFDQ
                     GLADLFTLRVAGNVLNDDILGSMEFATKVVGARLIVVLAHTSCGAVAGACKDVKLGHL
                     TDVINKIQPVVKPSMESTGIDNCSDPKLIDDMAKANALHVVKNILEQSPILNELVKNK
                     QIGIIAGLHDIKTGKVTFFEEKRSVPE"
     gene            1830..3050
                     /locus_tag="AC06IO_002408"
     CDS             1830..3050
                     /locus_tag="AC06IO_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIO9875580.1"
                     /translation="MPKSHNSIIFLGLLSAFSLLTFDLYQPSLPFITNYFNITPSMGQ
                     LTLSIYLLVYGITHLLWGPLIDHFGRRRLLPGTLLLALIGSLMCAFAPNITMLICGRA
                     LQGFALCCANLIAYSAARDFEDTIVRAKVLSYISMTVSVSPIIAPVIGALIFHHFGWQ
                     ANFIVMAIVACILLIQTKLSLLESPFWTPPQERFSVKKILQEYKSILNIPSLWCASFI
                     LMFGISAVMLTVINSSYLIIEVLNYSPLAYGLIFILNGLNIIFGNYLGIWLRDRLPII
                     TTIYLGNWFIVLGGTAMLVTSKLFGFSLLALSFSLIANLGISVSAAPTISIALTDFKQ
                     NAGIVMAFMNTIRLIGPSLLTILTGYLLTRNLDALPLGLIGSGIGALFFSWHFSRLTT
                     ESRNSDMDSEETLT"
     gene            complement(3047..4312)
                     /locus_tag="AC06IO_002409"
     CDS             complement(3047..4312)
                     /locus_tag="AC06IO_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phthiocerol/phthiodiolone dimycocerosyl
                     transferase family protein"
                     /protein_id="HIO9875581.1"
                     /translation="MKPPSRKLGYWEELCQWGNDKDHGNGLIAAVAKITVSDLDLERL
                     TNALRLLQIQQPLLRAKVIKSNQQCQFNIEEGDAVYLPFQIIKRTSNSVWKEVIELEL
                     NRPPRNQDYQWRTVLIYDQHGNSHEILLIAGHNIGDGISVAYFFDTLFRIYQKRDSNP
                     SFPLYPPVEELVQHKKGQKSSLPPAFSPTPIYYQNYVPPGERITKNHYYPVRVKQTGA
                     LLAFSKNHNFSLNALINALSLMTLTKVLDQPLDTTLHTPVNLRKWCIPVIPEDYFGCY
                     VSVVTTYHKQVSIESDIIELARDYDHQLKQERLPGDAIFPVHHSKQDIIDALSSWEGH
                     RDRFSMGVTVTNIGLLPFNTQYGHLKWEKSYFCTSRQNADIPVLLNVLTLNDQLMFCF
                     TFTEPMCGSVFQENYVKNFISNMGKLTDG"
     gene            4620..7448
                     /gene="ceg32"
                     /locus_tag="AC06IO_002410"
     CDS             4620..7448
                     /gene="ceg32"
                     /locus_tag="AC06IO_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg32/SidI"
                     /protein_id="HIO9875582.1"
                     /translation="MTKIYLLTAPFDEEAKTGDGQYAASLELGFAENYEHIPCKWLKK
                     DKGDYSIPFPTGTTSIPEETIPSAIVLQPVANENTVISGYKLKDAVSSPEKAQEVNNK
                     TVSPRTPKIIVKHDNSLQSLTIMDIYSQKPIQFDESKVDEIIHSLETKKVNLEKAIED
                     NNAELSKIKKQKSKLAYLTRLYKENKENIQDYCTLNEYIEAHLFNPKFLSRHEKALNN
                     FKALKSQFTGPVNLKELEKLTDKLTGIKEYSYDFHSNSLPYDLEHDKSFRNFYDFDGL
                     KESIKSIIKELEVLNSIRQAVSDKYPNSFKALNETEEHDDKLKFINIIFNDGFSTTYD
                     QQTFIKALSALDIEKAIDAYTNVKMKLENTQDIIANKEECRNKLISELQTLIANKQEP
                     YLSANEKLGGFFSKRKLSASEGFHLAYQANRRDPIKPEVIENIITKMKPLDEDTHLDI
                     HIRPPDCGVFITPEDIKKFQEAGIKVNITIHEYKQNYTRRYLQQYTHDLMRQANSVQF
                     FNAEDRENAIIAATYGDCDKRNTTEPTGVAKKIREVGEDFDLDKYPVQKYDLKGKSGL
                     TVASQKLSTEPDHPLDVVAKPPNILSFGTIRPGKGFEEALKLAQLTKDTSLSICEKIK
                     RVPIVKLAGDPQDKELMQQIVVERFGKAAVENYQKTHAYDNQFNNSQRRDYWKNLVRE
                     LNAKVKEEGAVLNNPYIEIYPWCEPHELLDLKQNCKYVCRMDDMGMRNNGSAIISVLD
                     VGVVYTKFGSVTDDIFIKGGKYGNAVDIGEYRYGKYSLLKKEKEFKEQHEEEPLPKWL
                     IKNPDSAYKRQSESRDPKDILDSIVAREENQLICDNIEDSDNYRTVVEAQKLLKERFT
                     LKNAVDHLLENIGLGHLIAQEEIDELFDTVDPVQAQIDNLDVLSDIKTPRLCLSRSCP
                     ELGFFGSRRGDLATKQENNKLKESILVF"
     gene            7450..8337
                     /gene="mesI"
                     /locus_tag="AC06IO_002411"
     CDS             7450..8337
                     /gene="mesI"
                     /locus_tag="AC06IO_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948207.1"
                     /note="regulates activity of cognate effector SidI;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS metaeffector MesI"
                     /protein_id="HIO9875583.1"
                     /translation="MVKGKLMPNFMKDVQKLIRDEGITDNVIATLSRLKPFDLAMLKA
                     TSDNKVKTLLDSDELKPFWVNKFNKLRLEKDHIFQFRNPDPQSRADFYCGYVLYLAAL
                     KEKQKEVSSYYDYLNLSFTTFNCFYAAQEILTFLIGACKNDTKRENIDLLYNFVTSQS
                     TQIQEHKTPGCLLLANAYFYLAGFYLRLDLKAESIECYKECWGQLHLAQLLETDSERE
                     IHNAYFNKGLATSNAFGLNSISEIKARCLELASEALPYPARNVIEANAEKTFENRFKD
                     SMSTAGKDDGDSITRPIIL"
BASE COUNT         2804 a         1541 c         1543 g         2559 t
ORIGIN      
        1 ggattaaata aaccagggaa aacgcgatca tgagcaaatt cctcgtattt attggatcaa
       61 tattattttt ttcttccgtt ttagctggag atatcccaga agtagacata caagatcaac
      121 agtcggatca ggagttatgt acccaaaaat tttataatca atgcatcaac aagtgcgaaa
      181 aaacaaatta tggcgactgt actcaagcct gtgaggaaaa tgctaaaaat caatgtcttt
      241 acgcaggtga ataagaatgg tttgctttga ggcaattaaa ttaaatccta taaattttaa
      301 tagtaaaaac attaactcat taaaaataaa ctgtatttcg tcatttcatg ttataaatag
      361 aacattgcat ctggaatttt aaaatgctcc ataataccaa aataaacaaa ctcgctgtct
      421 tttgtggatc taacactggt tattctgaag tctataaaca atccgctgaa aacatggccg
      481 atgtcctaag cagcaatggt attaccctgg tttatggtgg ctcaaaaagc ggtttgatgg
      541 gaataatagc caatcgcatg ttaaaaaatg gctctaatgt cattggtgtt ataccaaaat
      601 ctctggttga cacagaaaaa gcgcatgatg ggttgacgga attacatgtt gttaattcaa
      661 tgtatgaaag aaaagtattg atgtgtaaat tatcggacgg attcattctg ctgccaggcg
      721 ggtcaggttc tctggatgaa ttttttgaaa tgtttaccct ggtacaattg ggataccata
      781 aaaaaccttg ctgtgtactc aatgcctctg gctactatga ttaccttttg caatttttag
      841 actatgctgt taatcaaggt tttttacact caaaggatcg aaacgcgctc attgtcaatc
      901 aatatccaga tacgctcatt gagaatttat ttgaagtaat caataatcgc aaataacagt
      961 ttaggaaaat taataacata acaaggagtt aaccaatgcc caaaaaactg ctcgtcgctg
     1021 ctttcttatg caatattttt tgtagcccca gccatctcta tgcctcatca acagagatac
     1081 ccatacttgg gaaaaccatg acccaggcta aacaacaaca aatgactccc aagcaggcat
     1141 tgcaacgttt aaaagacggt aatcaacgct tcctcagtaa taaaccttta gcacgagatt
     1201 atctaaaaca agccaagcaa tcagcttatg gccaatatcc ttttgcggtc atcttgaatt
     1261 gtatggactc gcgcagtgtt cctgaattct tttttgatca aggattggca gacctgttta
     1321 ccttacgtgt tgccggcaat gttctcaacg atgacatttt aggaagtatg gaatttgcaa
     1381 caaaagtagt gggagcacgt ttgatagttg tcctggctca cacctcctgt ggtgctgttg
     1441 caggggcatg caaagatgtt aaattagggc atttgactga tgttataaat aaaatccagc
     1501 ctgttgtaaa acccagcatg gaaagcacgg gcattgataa ttgctctgac cccaaactga
     1561 ttgatgacat ggctaaagcc aatgccctgc atgttgtaaa aaatatacta gagcaaagcc
     1621 ctattttaaa tgaactagtt aagaataaac aaattgggat tatagcgggt cttcatgata
     1681 ttaaaacagg taaggtgact ttttttgaag aaaagcgatc tgtccctgag taaagcaagt
     1741 tggcagttgc caacttgctt taatatttcc tttcaaatgc aagccgaatt tggttacaat
     1801 cgcataattt ttggtgaatg attttttata tgcctaaatc acataattcc attatattcc
     1861 ttggtttgct ttctgcgttt tctttgctta catttgatct ttatcaacca tcactgcctt
     1921 tcataacgaa ttattttaat attaccccaa gtatggggca attaacccta agtatttatt
     1981 tgctagttta tggcatcact catcttctat ggggaccact tatagaccat tttgggcgcc
     2041 ggcgcctact tcctggaacc cttttattag ccttaattgg cagtttaatg tgtgcttttg
     2101 caccgaatat caccatgctt atttgcggtc gtgccctaca aggattcgcc ttatgctgcg
     2161 caaatctcat tgcctattcc gcagcacgtg attttgaaga cacgattgtt cgcgccaaag
     2221 tgctgtccta tatttccatg accgtttcag tttcaccgat tatagccccg gtaattggtg
     2281 ctcttatctt tcatcatttt ggctggcaag ccaattttat agtcatggca atcgttgcct
     2341 gtattctatt gatacaaacc aaactctcct tgttagagtc ccctttctgg acgccccccc
     2401 aggagaggtt ctcggtaaaa aaaatactcc aggaatataa gtccatactc aatataccct
     2461 ctctatggtg cgcatcattt attttaatgt ttggcatttc tgctgtcatg ctcactgtaa
     2521 ttaattcctc ttaccttatt atcgaggtac tgaattactc tccacttgcc tatggactta
     2581 tttttattct gaatggttta aatattattt ttggaaacta tctgggcatt tggcttcgtg
     2641 accgattacc cattataacc accatctatt tggggaactg gttcattgtt cttggtggta
     2701 cagcgatgtt ggttacctcc aaattgtttg gatttagtct gttagcattg tcattttcat
     2761 taattgccaa cttgggaatt agtgtcagtg ccgctccaac tatctccatc gccctaacgg
     2821 attttaagca aaatgcagga atagttatgg cttttatgaa taccatacgt ttaatcggcc
     2881 cctctctttt aactattcta accggctatt tgttgacaag gaatcttgac gcgcttccac
     2941 tgggattaat aggctcagga attggagccc tctttttctc atggcatttt agccgattga
     3001 ctactgaatc gagaaactcg gacatggata gcgaagaaac gttgacctaa ccgtctgtta
     3061 acttccccat attggatata aaatttttta cataattctc ctgaaacaca gagccacaca
     3121 ttggttctgt gaaggtaaag caaaacatga gttgatcatt aagagtaaga acattgagca
     3181 aaacaggtat gtctgcattt tgccgggatg tacaaaaata gcttttttcc catttcaaat
     3241 gaccatattg agtattaaaa ggtaacaaac caatattggt tacggtcact cccatcgaaa
     3301 agcgatccct atgaccttcc catgatgaca gagcatcaat aatatcctgc ttactatgat
     3361 gaactggaaa tatagcgtca ccaggcaatc gctcttgttt taactgatga tcataatccc
     3421 tggcaagctc tataatatct gattctatac tgacttgttt atgatacgta gtcaccacag
     3481 acacatagca gccaaaatag tcctccggaa tcacaggtat acaccacttc cttaaattaa
     3541 ccggggtgtg tagtgttgta tctaaaggct gatcgagaac ttttgttaat gtcattaggg
     3601 acagcgcatt aattaatgca ttcagagaaa agttgtgatt tttactaaaa gccagaagcg
     3661 ctccggtttg tttcactcta acaggataat aatgattttt tgttattcgc tcgccaggag
     3721 ggacataatt ctggtaataa atcggagtag gcgaaaatgc aggaggcaga gaagattttt
     3781 gccctttttt gtgttgcacc agttcctcta cagggggata gagagggaag gatggatttg
     3841 aatcacgttt ctggtaaatt ctaaagagag tatcgaaaaa ataggctacc gagatgccat
     3901 ctccaatatt atgtcctgcg attaacagga tttcatgaga attaccgtgt tggtcataaa
     3961 taagaacggt tcgccattga taatcttgat tacggggagg tcgatttaat tccaattcaa
     4021 tcacctcttt ccagacagaa tttgaagtcc tttttatgat ttgaaatggt aaataaacag
     4081 catcaccttc ctctatatta aattgacact gttgattgct tttgatcact tttgctctaa
     4141 gcaatggctg ttggatttgt aataacctga gcgcgttggt taaacgctcc aagtccaaat
     4201 cagaaacagt gattttcgca acagcagcaa ttaaaccatt tccatgatct ttatcgtttc
     4261 cccattgaca tagctcttcc caatagccta gttttctgga tggcggtttc atagattttc
     4321 tcccgaagcc ctggtcatga ttctggaaag ttccttttac agagagcaat ttccatacta
     4381 caggacatat acctgcttaa ttacttttga tggatctaac ccatattact gctctaaatt
     4441 ataaataatt gcttttttca agttctattt atgcaattaa gtcaaaaaaa ctcatcattt
     4501 tatcaacata ttatcatctt gctattcctg attatatttt gcaattatac ctcttaatgt
     4561 tcatttaata atcttaatgt aagatattca ccatgacaaa aattttacta aagtaattta
     4621 tgactaaaat atacttatta accgcacctt ttgatgaaga agcaaaaaca ggtgatgggc
     4681 aatacgcggc ctctttagaa ttgggttttg ctgaaaatta cgaacatatc ccctgtaaat
     4741 ggttaaaaaa agacaaaggt gattacagca tcccattccc aacaggaacc acttctatac
     4801 cggaagaaac gataccaagc gcaatagtat tacagccagt tgctaacgaa aatacagtaa
     4861 taagcggtta taaattaaaa gatgctgttt catctccaga aaaagcacag gaagtaaata
     4921 acaaaacggt cagccccaga acaccaaaaa ttattgtaaa gcatgataac agcctgcaat
     4981 cacttacaat aatggatatt tactctcaaa aacctatcca atttgatgag tcaaaagttg
     5041 atgaaataat tcactcttta gaaaccaaaa aagtaaacct ggaaaaagct atagaagata
     5101 ataacgctga gctgagtaaa attaagaaac aaaaatccaa attagcgtat ttgactagat
     5161 tatataagga aaacaaagag aatattcaag attattgcac cttaaatgaa tatattgagg
     5221 ctcatttatt taatcctaaa tttttaagca gacatgagaa agcactcaat aattttaagg
     5281 cacttaaaag ccaatttact ggccctgtca atttaaaaga actggaaaaa ttaacagata
     5341 aactgactgg aattaaagag tacagctacg atttccatag taacagttta ccctatgatt
     5401 tagagcacga taaaagtttc agaaattttt atgatttcga tggattaaaa gaatctatca
     5461 aaagcattat caaggaatta gaggtgctta acagcattcg tcaggctgta tcagacaagt
     5521 accccaatag ctttaaagcg ctaaatgaaa cagaagagca cgacgataaa ttaaaattta
     5581 tcaatattat tttcaacgat ggatttagca ccacttatga ccaacaaacc tttatcaagg
     5641 ccttatcagc tcttgatatt gaaaaggcaa ttgacgctta tacaaatgtt aaaatgaaac
     5701 tggaaaacac tcaggatatt atcgccaata aagaggagtg tagaaataag ttaatttcag
     5761 aattacagac tttaattgca aataaacaag aaccttacct ctctgcaaat gaaaagcttg
     5821 gcggtttttt cagtaaaaga aagctctcgg caagtgaagg atttcattta gcttatcagg
     5881 caaaccgacg tgacccgata aaacctgaag tgattgaaaa cattattacc aaaatgaagc
     5941 ccttggatga agatacccat cttgatattc atattcgccc accagattgt ggggtcttta
     6001 ttactcctga agacatcaaa aaatttcaag aggctggtat taaagtcaat atcacaattc
     6061 atgaatataa acagaactac actcgacgtt atttacaaca atatactcat gatttaatgc
     6121 gccaggcaaa cagcgtgcaa ttctttaatg cagaagatag agaaaatgct atcattgccg
     6181 ctacttatgg tgattgtgat aagcgcaata caactgaacc gactggtgtt gcaaaaaaaa
     6241 taagagaagt cggggaagat tttgacttag ataaataccc ggttcaaaaa tatgatttaa
     6301 aaggaaaatc aggattaacc gttgcatcac aaaaattaag cactgaaccg gatcatccat
     6361 tggatgtagt agccaagcct cctaatatct tatcttttgg tacgattcgt cctggcaaag
     6421 ggtttgaaga agcgttaaag ctggctcaat tgactaaaga tacctctctt tctatttgtg
     6481 aaaaaataaa acgggtacct attgtaaaac tggcaggtga cccacaggat aaagagttaa
     6541 tgcaacaaat agttgttgaa agatttggta aagcagctgt cgaaaactat caaaaaacgc
     6601 atgcctatga caatcaattt aataatagtc agcgcagaga ttattggaaa aatctggtta
     6661 gggagttaaa tgcaaaagtt aaagaagaag gcgctgttct taataatcct tacatcgaaa
     6721 tatatccttg gtgtgagccg catgaattgc tggatttaaa acaaaactgt aaatacgttt
     6781 gtcgaatgga cgacatgggc atgagaaaca atggctcagc aattatcagt gtattagacg
     6841 ttggagtcgt ttatactaaa tttggttctg ttacagacga tatttttatt aaaggcggca
     6901 aatacggcaa tgccgttgat attggagaat atcgttatgg gaaatacagt ctgttaaaaa
     6961 aggaaaagga atttaaagaa cagcatgagg aagaaccact acctaagtgg ctaatcaaaa
     7021 atccagactc tgcttataag cgacaatcag aatcgagaga tccaaaggat attttagact
     7081 ccattgtcgc aagagaagaa aatcaattaa tatgtgataa tatagaagat tccgataatt
     7141 atcgaacagt agtcgaagca caaaagttat taaaagagcg ctttacactt aaaaatgctg
     7201 tagatcattt attagagaat ataggactcg gccatttaat tgctcaagaa gagatcgatg
     7261 aattatttga tactgttgat ccagtacaag cacaaataga taatttagac gttctttctg
     7321 atattaagac accacgtctg tgtttatcac gctcttgccc tgaattaggt ttttttggct
     7381 cacgtcgtgg tgacttggcg actaaacaag aaaataataa acttaaagaa tcgatactgg
     7441 tattttgata tggtaaaagg aaaacttatg cccaatttta tgaaggatgt gcaaaaatta
     7501 atccgtgatg aaggcatcac agataatgtg attgcgactc tatccaggtt aaaaccattt
     7561 gatttagcca tgctcaaagc cactagtgac aacaaagtta aaacgctact ggatagtgat
     7621 gaattaaaac cattctgggt gaataaattt aataaattgc gattagaaaa agatcatatt
     7681 tttcaattta gaaacccgga tccgcaatca agagctgatt tctattgtgg atatgtttta
     7741 tatttagcgg cattaaagga aaaacaaaaa gaagtcagta gttattatga ttatcttaat
     7801 ctttccttca caacatttaa ttgcttttat gcggctcagg aaattttgac gttcttaatt
     7861 ggtgcttgta agaatgacac caaaagagaa aatatcgacc ttttatacaa ttttgtaact
     7921 agccaaagta ctcaaataca agaacacaaa acgccagggt gcttattgct tgctaatgcg
     7981 tatttttatc ttgctggatt ttatctgcgc ctggatctta aagcagaaag cattgaatgt
     8041 tataaagaat gctggggaca acttcatttg gcacaattat tagagacaga ttctgaacga
     8101 gaaattcaca acgcatactt taataaggga ttagcgacca gcaatgcatt tggtttgaac
     8161 tccatatcag aaattaaagc tcgatgcctt gaattagcct cagaagcatt accttatccg
     8221 gctcgaaatg tcatcgaagc aaacgctgaa aaaacatttg aaaatagatt taaagacagc
     8281 atgagtacgg cgggtaaaga cgatggggac agtataactc gaccaattat tttataatat
     8341 agtctgctga tattctcttt atggtattgt attatgatgc taaaaataac caacattagc
     8401 ttccaggtcg ctgtattttg cataggacaa ttcatattaa attaaag
//
LOCUS       DBNCWK010000129         8144 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.129, whole genome shotgun sequence.
ACCESSION   DBNCWK010000129 DBNCWK010000000
VERSION     DBNCWK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.129"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(71..1504)
                     /gene="gatB"
                     /locus_tag="AC06IO_002412"
     CDS             complement(71..1504)
                     /gene="gatB"
                     /locus_tag="AC06IO_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIO9875584.1"
                     /translation="MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGL
                     PGVLPVLNEQAIIMAIQFGLAIHGTINDLSVFERKNYFYPDLPKGYQISQYQKPIVTN
                     GYLNIQLGNNLEKTVHIARAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCL
                     HSAEEAINYLKTLHQLVRFLGICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLN
                     SFRFIEKAIAFEQARHQDILESGLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDP
                     DLLPIHIDKEQIEAIKNNLPDLPEAISKELKNTPSLNDEDINFILSSPDTYQYYKKIK
                     SLCPAADKTIINWLKGQYAAFLNEHNLTFETPPISAKTMAAFLSKIHEKKISSSIAKN
                     IFSMLCVGEEDIDAIIEREGYQQQNDNSALEEIVEQIIKQYPDQVTEYKAGKEKLLAF
                     FIGQAMKQTKGKANPEQINLLLKKHLG"
     gene            complement(1508..2959)
                     /gene="gatA"
                     /locus_tag="AC06IO_002413"
     CDS             complement(1508..2959)
                     /gene="gatA"
                     /locus_tag="AC06IO_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIO9875585.1"
                     /translation="MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLD
                     EDQALKEAQSADLVLKNGEGKPLTGIPMALKDLFCTKRLNTTCASKMLANFQAPYDAT
                     IVTKFKQNGAIIIGKTNMDEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAG
                     NLVPFAIGSDTGGSIRQPAAFCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAED
                     LAMILHCIAGFDSKDSTSVDRVIPDYSAEIKKPVDKIRIGLPSCFFQPQVEKGIQDAI
                     HDAVKLFENLGAEIIEIDLKLQPLWVPCYYVIACAEASSNLSRYDGIRFGHRSKSAST
                     LIELITNSRSEGFGNEVKRRILTGTHVLSTGFFNAYYLHAQKVRRLIRDELITTLNSV
                     DVIIGPTTPTTAFKLGEKINDPIQNYLADVFTVAANLAGLPAISIPTGFENKLPIGLQ
                     LMGKHFSESRLLAIAHHYQQHTNWHLANPNKQG"
     gene            complement(2962..3258)
                     /gene="gatC"
                     /locus_tag="AC06IO_002414"
     CDS             complement(2962..3258)
                     /gene="gatC"
                     /locus_tag="AC06IO_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIO9875586.1"
                     /translation="MTLSAKDLENISKLAYLNEDSEHSAKLTQEVSAIMDFVDQLKTI
                     DTSQIAPLFHPLALNQRLRPDEVTEAECLAELEAMAPLFEDNLYLVPKVISSDK"
     gene            3532..5679
                     /locus_tag="AC06IO_002415"
     CDS             3532..5679
                     /locus_tag="AC06IO_002415"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIO9875587.1"
                     /translation="MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASS
                     FEYPGRYTCWDMGFYNPPLVMICQNNTICFEALNQRGEILLSFLYPLLKTTDLWEVIS
                     QTTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLI
                     YQFEQLERCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREG
                     KYSPYQPIHKPEKNCDHEPGEYAEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSIL
                     FRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVETCPISGTIKRGADAIED
                     AHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHV
                     EGILRDGFDAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGN
                     LNTGLVLRTVRIEKGTAEIRVGATLLYDSIPEAEEEETRLKASAFLDILQKPWQKAKK
                     KTEDVPLVGKNKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGYLQNNHFDLI
                     VLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLGLQGIVEYFGGVLDVLEYPMHGKSS
                     VIRVLDNKGLFSGLGDCFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHEYLPV
                     HAVQFHPETILSLSDQVGLRIIINLMNMVKDNG"
     gene            5672..6955
                     /gene="clcA"
                     /locus_tag="AC06IO_002416"
     CDS             5672..6955
                     /gene="clcA"
                     /locus_tag="AC06IO_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HIO9875588.1"
                     /translation="MGKKILILYFVSVLLGILTGAIASLFQLTIQQMDHLLELLFHMT
                     ESYGLPVGIVSAMTSMVMLFIAWMMVKYIAPEAAGSGVQEIEGTLLHVRPIFWKRLIP
                     VKFFGGVLSISAKMVMGREGPTIQMGGNLGQMLGELFRLTRRRRDTLIAAGSAAGLAA
                     AFNAPLAGVLFVMEEMRNQFNYSFTNFKMVVICCVMATIVLHLIIGSQPAIQMEVFEL
                     PTLGSLWLFFVFGILMGLAGLLFNVTLMRTVGWLDKQNPKNKKYYILAVGFLVGYLAY
                     SYPATVGGGYVIISEALTMSPAFSVLCSLIVIRFIMTMLSYSSGVPGGIFAPMLALGT
                     LFGLGFFHLFQWLSMDFSIEPGMFAVAGMGALFAAAVRSPITGVVLVVEMTKNYSLIL
                     PLMVSCITATIVVQLARNEPIYTQLLERTLRLNAK"
     gene            6962..7108
                     /locus_tag="AC06IO_002417"
     CDS             6962..7108
                     /locus_tag="AC06IO_002417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875589.1"
                     /translation="MSQYVLIVQLWGIRIKVRRALDLSTKNTNSLDIAKAALEESVRI
                     ILSS"
     gene            complement(7129..8130)
                     /locus_tag="AC06IO_002418"
     CDS             complement(7129..8130)
                     /locus_tag="AC06IO_002418"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIO9875590.1"
                     /translation="MHSYLKKDLILHYIQINNPGPHSELTLAEGPNPGFNESQILVRV
                     KATALNRADLMQKYGKYPPPPGESDIPGLEVAGEVIVTGSKVKQFKPGDKIYGLVGSG
                     GYAEICPVEASLAHHIPSHWNYSLAAALPEALTTVYATLYDLGALSAGQTLLIHGAGS
                     GIASLAIQMAKLTAAKVITTVGDSSKIEKALALGADQVILYKEQDFELLIEDNSVDLI
                     IDFVGGDYFNKHLHLLKPQGKLIQIACLKGSKVECSLALLMQKRLHIIGFVLRSQSIK
                     EKARLWKLAHDHWFNAFENDKIKPVIDSEFDLNQIETALQRMQQGSHFGKIVIRVGK"
BASE COUNT         2355 a         1448 c         1705 g         2636 t
ORIGIN      
        1 aaagatgata atagctggca atcaatcaac aaagccataa atgatcattg tgaattataa
       61 aaatattgca tcatccaaga tgttttttta ataacagatt gatttgctcg ggatttgctt
      121 tccctttagt ttgtttcatc gcttgcccga taaaaaaagc taataatttc tctttaccgg
      181 ctttgtattc tgtaacttgg tcgggatatt gtttgattat ttgctcaact atttcttcca
      241 gtgccgagtt gtcattctgt tgctggtagc cttcgcgttc aataatcgca tcgatatctt
      301 cttcacctac acaaagcata ctgaaaatat ttttagcaat actggaagag atttttttct
      361 catgaatttt gctaaggaaa gcagccatgg ttttagctga aattggtggt gtttcaaagg
      421 ttaggttgtg ttcattaaga aaggcggcat attggccttt taaccagttg ataattgttt
      481 tatcagcagc cggacaaagg gatttaattt ttttgtaata ttgataagtg tccggtgatg
      541 ataatatgaa attaatgtct tcatcattta gggacggagt gttttttaac tctttggaaa
      601 ttgcttcagg caagtccggc aggttatttt taatcgcttc aatttgttct ttgtcaatat
      661 gaattggtaa taaatcagga tcaggaaaat aacggtaatc attttcattt tccttgcctc
      721 tcattgcctg agttgtgtta ttgtcaggat tgtaaaggcg agtctcttgg atgactgaca
      781 gaccgctttc cagtatatct tggtgtcttg cctgttcaaa agcaatggct ttttcaataa
      841 aacgaaatga attcagattc ttaagttctg ttcgtgtgcc taaaactgag ctgcctttag
      901 gttttataga cagattgacg tcacaccgaa aagaaccttc ttgcatattg ccatcgcata
      961 ttccaaggaa tctcactaat tgatgcagcg tttttaaata attaatcgcc tcttcagcgg
     1021 agtgcaggca aggggttgtg actatttcca atagtggggt acctgctctg ttgaggtcga
     1081 tcccggtgta atcagtatgg gcatcgtgta gggatttgcc agcatcttct tctaaatgag
     1141 cgcgtgcaat atggactgtt ttttccaggt tattaccaag ctgtatattt aaataaccat
     1201 tagtaacaat gggtttttga tactgactga tttgatagcc cttgggcaag tcaggataaa
     1261 aataattttt acgctcaaaa acagatagat cattaatggt tccatgaatt gccaaaccaa
     1321 attggatagc cattatgatg gcttgttcgt ttaatacagg gagaacacca ggcaaaccag
     1381 catcgataaa actcgtttgc gagttagggg tggcaccaaa agcagtagat gctcctgaaa
     1441 ataattttga tttggttttt aattgagcat gtacctcaag accaatgacg gtatcccatt
     1501 ccatatatca cccttgcttg ttaggattgg ctaaatgcca gttggtgtgt tgctgataat
     1561 gatgtgcgat tgcaagcaag cggctttcac taaagtgctt acccattaat tgcaaaccaa
     1621 ttggtagttt attttcaaaa ccagttggta tagaaatagc tggtaggcct gcaagattgg
     1681 ctgctacagt aaatacatct gccaaataat tttgtatggg atcatttatt ttttcaccca
     1741 atttaaaagc ggtggtaggg gtcgttggcc caatgataac atctactgaa tttaaagttg
     1801 taattaactc atctcgaatc aaacgcctca ctttttgtgc atgcaaataa taggcattaa
     1861 aaaaaccggt tgagagcaca tgagttccag ttagaattcg acgttttact tcgttaccaa
     1921 aaccttcact tcgagagtta gtgattaatt cgattaaggt tgatgcggat ttgcttcggt
     1981 ggccaaatct tatgccatca taacgcgaaa gatttgatga agcttctgcg caagctataa
     2041 catagtaaca tggcacccaa agtggctgaa gtttcaagtc gatttcaatt atttcagccc
     2101 caagattctc aaataatttc acagcatcat gtatggcatc ctggatacct ttttcgactt
     2161 gaggttgaaa aaagcaagaa ggaagtccta tgcgtatttt gtctactggt tttttaattt
     2221 ctgctgaata atcaggtata actctatcaa ctgatgtgga atctttacta tcaaaccccg
     2281 caatacagtg aagtatcatg gccagatctt cagctgactt cgcaaatggt cccgcttgat
     2341 caaggcttga ggcaaaggcg accattccgt atcgagagac taatccatag gtgggtttta
     2401 tcccgctaat cccgcaaaag gctgctggct gacgaattga tccaccggtg tcagagccaa
     2461 ttgcaaaagg taccagattt ccagcaaccg ctgctgcaga accgcctgat gatccacctg
     2521 gcactctttc tctatcccac gggtttttta ccgaaccgaa gtaactgttt tcattagagg
     2581 atcccatggc aaactcatcc atattggttt ttccaataat tattgcgcca ttttgtttaa
     2641 attttgtgac gatagtggca tcataggggg cttgaaaatt agccagcatt tttgaagcac
     2701 aagttgtatt taatcgcttt gtacaaaaca aatccttgag agccatggga atcccagtca
     2761 gcggctttcc ctctccattt tttagtacca aatcagccga ttgcgcttct ttaagagctt
     2821 ggtcttcatc aaggctgatg aatgcattca agtctttatt gctttgaatt ttgttaatac
     2881 agtgttgtgt taattcaaca cttgaaaatt ctcgattatg aagagccttt gataattgag
     2941 ccagtgagta atgttccatt attatttatc cgatgatatc accttgggaa ccaaatataa
     3001 gttatcctca aataatggtg ccatggcttc taattcagca agacattccg cttcagttac
     3061 ttcatctggt cttaagcgtt gatttagtgc caagggatga aaaagaggtg ctatttggct
     3121 tgtatctatt gttttgagct ggtcaacaaa atccatgatc gcacttactt cttgagtcaa
     3181 ttttgcagaa tgctcagagt cttcatttaa ataagccaat tttgaaatat tttcaagatc
     3241 tttagcagag agagtcatgg tcatatccaa atataagggt tatacattat agcggtattg
     3301 ttctaaaaat gaagtattag tttaattatc ttttagtctg ttgagccaag ttgctttcct
     3361 agagccatgt ttaatcatga ctgcagtcat gtcttttttg aagcaagttt tgcaatctct
     3421 tctgaagaca taaaaaagca atgatattga aatgttacaa gattcttgca gaacagtggt
     3481 tttacagtag aatgaatatt tgtacgaatt aaatgataga gaatggtatt catgttacaa
     3541 caatataaaa ctctcggtgg tgttcatgtt gagttttctc aacaagagtt ggattatcaa
     3601 caaggcatag attttttgct tgagagtctt gattctcaac gtggagcagt atttgcttcg
     3661 agctttgaat atccgggacg ctatacttgt tgggacatgg gtttttataa tcccccatta
     3721 gttatgatct gtcaaaataa taccatatgt ttcgaggcat taaatcaacg gggcgagata
     3781 ttattatctt ttctttatcc gttgttaaaa acaacagatc tctgggaggt aattagccaa
     3841 acgacctcat tttgtcagtt aaaaattaaa tcatcagaca aagtatttag tgaggaggag
     3901 cggagccatc aaccgtcagt attcagtgca attcgcctct tattgaattt ttttaaaaca
     3961 gaagaagatt catacttggg tttatatggt gcttttgggt ttgacctgat ttatcaattt
     4021 gaacagttgg agcggtgtaa accaagaaat gaaacacaga gagatatggt tctctatcta
     4081 cctgatgaaa tttatatagt gaatcatcgt aaggaagaag cctttgttag acgctatgat
     4141 tttcaatatc aggggcgatc aactcaatct ttatccagag aagggaagta ttcaccatat
     4201 caacctatcc ataaaccgga aaaaaactgt gatcacgagc caggtgaata tgccgaagtg
     4261 gtcaaaaaag ccaaagaaaa attttcctgt ggtgatttat ttgaagtggt tcctagccaa
     4321 actttctata ctcactttgc tgaaaagccg tcaatattat ttcgccaaat gcgagaaata
     4381 aatccatcgc catatggatt ttttgttaat ttgggtgacg aagaatatct ggtaggtgct
     4441 tcacctgaaa tgtatgttcg tgtccaaggt aaaagagtag aaacttgccc tatctcaggc
     4501 actatcaaac ggggagcaga tgctatagag gatgctcaca atattcaaac acttctagat
     4561 tcagagaaag aagaatcaga actaaccatg tgcaccgatg tggatagaaa tgataagtca
     4621 agaatatgcg aagcagggag tgtgaaagtc attgggcgtc gccaaatcga aatgtattcc
     4681 cgattgattc atacagttga tcatgttgag ggtattttaa gagatggatt tgatgcagta
     4741 gatgcttttt taactcatat gtgggttgtt actgtaactg gtgcgcctaa aatttgggct
     4801 atgaatttca ttgaacaaca tgagaaatcc ccacgtaagt ggtatgcagg ggctgtagga
     4861 tggtttggtt ttgatggaaa tttaaatact ggattggtct taagaacagt gcgaatagaa
     4921 aagggaactg cagaaattag agtaggcgca acgttacttt atgactctat ccctgaagct
     4981 gaggaagagg agacgagatt aaaagcttct gcttttttag acattttaca aaaaccatgg
     5041 caaaaggcta aaaaaaagac agaagatgtt cccctggtag gcaaaaataa aaaagtttta
     5101 cttatcgatc atcaagattc ctttgttcat actttggcaa actatttaag acaaacggga
     5161 gctgcggtta caacagttcg ctatgacaag gcgattggct atttacaaaa taatcatttt
     5221 gatttgatcg tattatcgcc tggccccgga aaaccggttg attttaagct ttcagaaaca
     5281 atcgatgtga tactctcaaa gaacatacca atattcggtg tttgtctggg gctgcaagga
     5341 attgttgaat attttggagg ggttttggat gttcttgaat accctatgca tggaaaatct
     5401 tcagtaatca gggtattaga taacaagggg ttgttctccg gtttaggtga ttgttttaaa
     5461 gcgggaagat accattcact ctatgccaga gcagaggctt tacccaatga attattagtt
     5521 accgctgtaa gtgaagatga tgtgattatg gcagtttctc atgagtattt acctgtgcat
     5581 gcggttcaat ttcatccaga gacaattttg tctttatcgg atcaagtggg tttaagaata
     5641 atcatcaatt tgatgaacat ggtgaaggac aatgggtaaa aaaatactga ttttatattt
     5701 tgtatcagtt ctattaggga tattaacagg cgcaattgct tcattattcc aacttactat
     5761 tcaacagatg gatcatctgc tggagctatt atttcatatg acagaaagtt atggacttcc
     5821 tgtaggaatc gtttccgcta tgacatcgat ggtgatgtta tttattgcct ggatgatggt
     5881 gaagtacatc gccccagaag cggcaggaag tggtgtgcaa gaaattgaag gtaccttgct
     5941 tcatgtgaga ccaatatttt ggaagcgtct gataccagta aaattttttg ggggtgtgtt
     6001 atccatatcg gcaaaaatgg ttatgggaag agaagggcct actattcaga tgggtggtaa
     6061 ccttggacag atgcttggag agttgtttcg gcttacacgc cgtcgccgtg atactttaat
     6121 agccgctggt tctgccgctg gtttggctgc agcatttaat gcgcctctgg ctggtgtact
     6181 gtttgtcatg gaggaaatgc gtaaccagtt taattattca tttaccaatt ttaaaatggt
     6241 cgttatttgt tgtgttatgg caacgattgt tttgcatctt attattggct cacaaccggc
     6301 tattcaaatg gaggtttttg aactacctac actgggctct ttgtggctgt tctttgtttt
     6361 tggaatatta atggggctgg ctggcttatt attcaatgtc acactcatga gaactgttgg
     6421 atggttagat aaacaaaacc ccaaaaacaa aaaatattat attttagctg ttggattttt
     6481 agtcggttat ctggcgtact cctatcctgc tactgttggg ggcggttatg ttatcatcag
     6541 tgaagcactg actatgtcac ctgcatttag cgtgttatgc agtcttattg taatcaggtt
     6601 tatcatgact atgttatcct attcaagtgg cgtgcctggt ggtatctttg caccaatgct
     6661 ggctttggga acactatttg gattaggttt tttccattta tttcaatggt taagtatgga
     6721 tttttcaata gagcctggta tgtttgcagt cgcaggtatg ggagcgcttt tcgcagctgc
     6781 agtaagatca ccaattactg gcgtggtatt ggttgttgaa atgactaaaa attactctct
     6841 aattctacct cttatggttt cctgcattac tgcaacgatt gtggtgcaat tagcacgtaa
     6901 cgagccgatt tacactcaat tattagagcg aactttacga ctcaatgcaa aatgagtcta
     6961 aatgtctcaa tatgtattga tagttcagct atggggaata aggataaaag taagaagagc
     7021 ccttgattta tctaccaaaa acacgaactc actggacatt gccaaggcag ctttggaaga
     7081 gtctgtgcgt ataatattga gctcatgaga aattgatgag gcttagcatt acttacctac
     7141 gcgaatgact attttcccga aatgagagcc ttgttgcatg cgttgcagtg ctgtttctat
     7201 ttgatttaga tcaaattccg agtcaattac tggtttaatc ttgtcatttt caaatgcatt
     7261 aaaccaatga tcatgagcca atttccaaag ccttgctttt tctttgatgc tttgcgatct
     7321 gagtacaaat ccaatgatgt gcaacctttt ttgcataagc aacgccagac tgcattctac
     7381 tttgcttcct ttgaggcaag caatttggat taatttaccc tgaggcttca ataaatgtaa
     7441 gtgtttgtta aaataatcac caccgacaaa atcgatgatc aaatcaacag aattgtcctc
     7501 aattaataat tcaaaatcct gttctttgta aagaatgact tgatcagcgc ccagggcaag
     7561 agccttttct attttgctgg aatcaccaac tgtcgttatc actttagccg ctgttaattt
     7621 agccatttgt atggctaatg aggctattcc gcttccagcg ccatggatca ataatgtttg
     7681 tccggcactt aaggcaccta aatcgtacaa cgtagcataa actgtcgtta acgcttccgg
     7741 gagcgccgcg gccaatgaat aattccaatg agaaggaata tgatgagcta aagaagcttc
     7801 tactggacag atttcagcat aacctccact cccgacaagg ccgtaaattt tatcacccgg
     7861 tttaaattgt tttaccttgg aaccagtgac gattacttcc ccagctactt ctagtccagg
     7921 tatatcggat tctcctggag gagggggata tttaccatat ttttgcatca aatcagctct
     7981 attcaaggct gtggctttga cacgcacaag aatttgagat tcattaaaac caggatttgg
     8041 accttcggca agggttaatt cactatgagg tcctggatta ttaatctgta tgtaatgcaa
     8101 gataagatcc tttttaaggt aagaatgcat agtatgataa ttta
//
LOCUS       DBNCWK010000130         8118 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.130, whole genome shotgun sequence.
ACCESSION   DBNCWK010000130 DBNCWK010000000
VERSION     DBNCWK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.130"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            120..1127
                     /locus_tag="AC06IO_002419"
     CDS             120..1127
                     /locus_tag="AC06IO_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875591.1"
                     /translation="MTRLEELLYTLTSVIIRYHDSQPKVLNKLIVESNPILLRRKALA
                     LAKEIIQDKDIECKGRLEELIQNCTKGYPDRKPFLYFILNEVLFLKSMLDRKSSFEPS
                     KLEGYKKQIAQLLIDLKQLMITPKSKTYEVKYTSATENSETSISLSGVKNDGYVGGEF
                     CNSGNLIKEEVLEALNIHINSKNTAECSSDEEIKALAESLCLEHQNALLVPELSEKAR
                     ILTESSQEQENELVTKTKQIETDKKTILEQQATIQEKDKEIEALKEKISDLTRTAPRP
                     VFNPVLPMGYSSLFFNTRLGTQAKQQTTFDVTDLPIIKSSESKDAEPNKSFSMGNSHG
                     D"
     gene            1279..3663
                     /locus_tag="AC06IO_002420"
     CDS             1279..3663
                     /locus_tag="AC06IO_002420"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIO9875592.1"
                     /translation="MKKMAYFWRKGLWALMSLFFLLIVAGSLLYLYLESQLPNVDSLK
                     TVQLQVPLQIFSKEGLLIQEYGEKKRIPVTYDEIPPTLIHALIATEDQRFFEHPGVDV
                     MGLGRAAVSMLKTGTKSQGGSTITMQVARNFFLSRKKTFLRKFNEIMLAIKIDRELSK
                     EKILELYLNRVYLGNRAYGVGAAAMVYFGKSLKDLNLAELAMIAGLPQAPSTQNPIAN
                     PLAAKKRRDHVLERLLEEHYINEEQYQNAVNQPITAKYHGTNIKVKAPYVAEMIRQSL
                     YDNFGPEAYTKGYKVYTTIDGKLQNTANEVVEKNLIAYDHRHGYRGPIANIGEKDSQS
                     PKIRQKYLRQYPELNSLIPAVVTEVREKEATAALQNGQIIIIPWEGMSWARPALKRGG
                     WVGKSPTKAQQVVAAGDIIYVHPTEKSWQLAQIPEAESAMVAMNPKNGAIEVLVGGFN
                     FSKSKFNRATQSSRQPGSSFKPFVYAAALNNGYNLATLINDAPIVVDDPSQPNLWRPH
                     NVNLKFNGPTRLKQALVQSKNLVSIRILDDIGIDYTIDFLTRFGFNKKSLPRALSLAL
                     GSLSISPMDLTTAYAVFANGGYKVEPYLIDHITDADGKILLQAKPSVVCDPCDKDKVD
                     ASTLAPRVISEDIAFLMNTALRDVIQHGTGRAARVLNRQDIAGKTGTTNDQVDAWFAG
                     FNSNLVVTTWIGFDNPKSLHEYAANLALPLWIDFMKVALKDKPETELKQPENVIAVRI
                     DPVSGLLAKPNQENGIIEYFRQNEVPSEEDQGPVYNAHNDQQEEELAPVEESLF"
     gene            complement(3735..4856)
                     /gene="ald"
                     /locus_tag="AC06IO_002421"
     CDS             complement(3735..4856)
                     /gene="ald"
                     /locus_tag="AC06IO_002421"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIO9875593.1"
                     /translation="MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIG
                     ISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPH
                     QARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
                     GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTV
                     YSTADSIEKYVSSADLVIGAVLVPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFE
                     TSRATTHQEPTYVVDNVVHYCVANMPGAVPRTSTFALNNATLPFILSIVTKGVKLALL
                     SDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR"
     gene            complement(4868..5806)
                     /locus_tag="AC06IO_002422"
     CDS             complement(4868..5806)
                     /locus_tag="AC06IO_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIO9875594.1"
                     /translation="MSTLVLVEHNNQQLHPATRNTLSAALELGDKITLLVVGYQCRSV
                     AEEAASLEGAHAVLHVDNPCYEHQLAEAVSDLVVSFANSFSAILAPSSTYGKNILPRV
                     AAQLDVTQISDVSKIINSDTFEHPVYAGNAIETVRVLDSLKVMTIRTTAFDPITSSQE
                     VCCIESIDKEFQAQGSQFVRHELSKSERPDLGSAKIVVSGGRGLQNAEKFKLIEELAD
                     TLGAAVGASRAAVDAGFVPNDYQVGQTGKVVAPMLYVAVGISGAVQHLAGMKDSKVIV
                     AINKDADAPIFQIATYGLVGDLFELVPQLIEQLKNR"
     gene            complement(5820..6569)
                     /locus_tag="AC06IO_002423"
     CDS             complement(5820..6569)
                     /locus_tag="AC06IO_002423"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIO9875595.1"
                     /translation="MKILVAVKRVIDPYVKIRVKSDNSGVETQNIKMAMNPFDEIAIE
                     EALRLREKNWATEVIAVSIGADSSQETLRHALALGADKAILVRTEKSFESLNIAKILN
                     NIVANEKPDLVLMGKQSIDGDNNQTPQMLAALLNWPQATNASKIIPDNNNLEVVREID
                     GGLETLKVQLPAVISTDLRLNEPRYASLPNIMKAKRKPLDIIDLDSLGLSLKQHVEIL
                     KVNAPATRSGGVILDSVAALLDKLQHEAKVL"
     gene            complement(6978..>8118)
                     /locus_tag="AC06IO_002424"
     CDS             complement(6978..>8118)
                     /locus_tag="AC06IO_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIO9875596.1"
                     /translation="LERDFARFINQQLLIDGPEKEKIVKNYKRLTLCFHPDHASGFSP
                     EMVWLENNLSQSKNNGACFKILGLCYEKLISPEKFKDSSLGDIKSKDDFKQWLENLRS
                     KATTYSGQSFCDSLINLLEQSSGFFDDTGKIKPRGLRVLLRFIPVVFASYGTFLFAEE
                     LFAIYALYFILLKGGQYMESTESSQLKKIGCTLQEISIITATASTTLLVRLLEMTFWA
                     SRQFLDVSLQIGSAILKPMLPAPETKEKSESDTAANLCRDLILASHNTGEGMQFKTPE
                     LKVISAPLESYLGLNAQQFFGDLRIGREKRLKVEAFLFKMRVLDSLPSPLEEKLVEAQ
                     KELDKIKKDTKVFTSNTAKAVKEAEQVISLLQDKDSMQLVVYNGT"
BASE COUNT         2583 a         1712 c         1463 g         2360 t
ORIGIN      
        1 aaataatgta ccagttaaca agcacttgca cttatgcaaa acattcaata tagtgaaata
       61 ccaagttgta ggggatttcc acatttagat ttttagcttt ataaaaagga gctttacata
      121 tgactagatt agaagagcta ctctatacac tgacttctgt aatcattcgg tatcatgaca
      181 gccagcctaa ggtattaaat aaattaatag ttgaaagcaa tcccattctc ttaagaagaa
      241 aagcacttgc tttggctaaa gaaattattc aggataaaga cattgaatgt aagggacgcc
      301 ttgaggaatt aatacaaaat tgcacaaaag gttaccctga cagaaaacca ttcttatatt
      361 ttattttaaa tgaagttctt tttctaaaat ccatgctgga taggaaaagt tcatttgagc
      421 catctaaact ggaaggatat aaaaaacaga tcgctcagct tttaattgat ctcaaacaat
      481 taatgatcac ccctaaaagt aaaacttatg aagtaaaata tacttctgct actgagaact
      541 ctgaaaccag catttctctt tcaggcgtaa aaaatgatgg atacgttggt ggagaatttt
      601 gcaactccgg aaatttgatc aaagaagaag ttttagaagc attgaacatc catattaatt
      661 ctaaaaatac agcagaatgt tcttctgatg aagaaattaa agcgcttgct gaatctttat
      721 gtttagagca tcaaaatgcg ctcttggtac ctgaattatc ggagaaggcc cgaatattga
      781 ctgaaagcag ccaagaacag gaaaacgaac tggtaacaaa aacaaaacaa attgaaacag
      841 ataaaaaaac aattttagaa caacaagcta caattcagga gaaagacaaa gaaatagaag
      901 cactgaaaga gaaaataagc gatttaacgc gcactgcacc ccgccccgtt ttcaatccag
      961 tcttacctat gggatacagc tctctgtttt tcaataccag attgggtact caagctaaac
     1021 aacaaacaac tttcgatgta actgatttac caataattaa gtcctcagaa tcaaaagacg
     1081 ctgagcctaa caaatcgttt tctatgggta atagccatgg agattaaagt ctattggcgc
     1141 ttcatcgatg attatctcca tagctgtgag gcgcaaaata gcatttaaat caggtttttt
     1201 tcccttgtaa acctcgcaat attatataga ttcgctgtat aatattgcac tactcacatt
     1261 taacgaaaac gaaattctat gaaaaaaatg gcatacttct ggcgtaaagg tctttgggcg
     1321 ctgatgagcc tgttttttct cctaatagtg gctggcagcc ttctatacct ttatctcgaa
     1381 agccaattgc ctaatgttga ttcattaaaa actgtacaat tacaagtgcc attacagatt
     1441 ttcagcaaag aaggtttatt aattcaggag tacggtgaaa aaaaacgtat tcccgtaact
     1501 tatgacgaaa ttccccctac tctgatccat gcattaattg caacagaaga tcaacgattt
     1561 tttgagcacc ctggtgtaga cgttatggga ttgggccgag ccgcagtaag catgctcaaa
     1621 actggaacca aatctcaagg tggcagtacg atcaccatgc aagtagctcg taattttttc
     1681 ttaagccgta aaaaaacatt tttacggaaa ttcaatgaaa ttatgcttgc tatcaaaatt
     1741 gaccgggaat taagcaaaga aaagattttg gaactttact taaacagggt ttatctgggt
     1801 aatcgggctt acggtgttgg cgcggcagcc atggtttatt ttggaaaatc ccttaaagac
     1861 cttaatctgg ctgaactggc tatgatagca ggacttcctc aagctccctc aactcagaat
     1921 cctatcgcta acccgcttgc tgcaaaaaaa cgtcgtgacc atgtactgga gcgattactg
     1981 gaagaacatt atatcaatga agaacaatac caaaatgcag tcaatcaacc aattactgca
     2041 aaataccatg gaactaatat caaggtgaaa gcgccttatg ttgctgaaat gattcgtcag
     2101 tctctgtacg acaattttgg ccccgaagcg tacactaaag gctataaggt atatacaacc
     2161 attgatggca aattacaaaa tactgccaat gaagtggtag aaaaaaatct aattgcttac
     2221 gatcatcgac atggttacag agggccaatt gccaatatag gtgaaaaaga cagtcaatct
     2281 cccaagatta ggcaaaaata cttgcggcaa tatccagagc tgaacagtct gattccagca
     2341 gtggtcactg aagtaagaga aaaagaagcg actgcggcat tacaaaacgg gcaaataatt
     2401 attattccct gggagggtat gtcctgggcc aggccggcac tgaaaagagg aggctgggtt
     2461 gggaaatcac caaccaaagc acaacaagtg gtagcagcag gcgatataat ttatgtacac
     2521 cccacggaaa aatcctggca actcgcccaa attcctgaag cagaatctgc aatggttgct
     2581 atgaatccta aaaatggcgc cattgaagtt ttggtcggag gatttaactt ctcaaaaagt
     2641 aaattcaacc gagctaccca atccagcagg caaccaggct ccagtttcaa accatttgtt
     2701 tacgctgcgg ctttgaacaa tggttataat cttgctacct taattaatga tgcccccatc
     2761 gttgtagacg atcccagcca accgaattta tggcggccac acaatgtgaa tctaaaattc
     2821 aatggcccta cccgcctgaa acaggcatta gttcaatcta aaaatttggt atctataaga
     2881 attcttgatg atataggtat tgattatacg atagatttct taactcgttt tggctttaat
     2941 aaaaaatcat tgcctagagc cttatcactt gctttaggaa gtttgtccat tagccctatg
     3001 gatttaacca ctgcttatgc agtctttgct aatggtggtt ataaagtaga gccctaccta
     3061 attgaccata taacagacgc tgatggtaaa attctgttac aagccaaacc aagtgttgta
     3121 tgtgaccctt gtgataaaga caaagttgat gcctctacct tggcacctcg tgttatttct
     3181 gaagacattg cctttttaat gaatacggca ttacgcgatg taatacagca tggcactgga
     3241 cgtgctgcac gagtattaaa tcgtcaagac atcgctggta aaacagggac tactaatgat
     3301 caagtagatg cgtggtttgc cggatttaac tctaatttgg tagtaacaac ctggattggt
     3361 tttgataatc ccaaatcatt gcatgaatat gctgcaaatc ttgctcttcc gctatggatt
     3421 gattttatga aagtagcatt aaaagacaaa cctgaaactg aattgaagca gcctgaaaat
     3481 gttatagctg ttcgaataga tcccgtaagt ggtttgctcg ccaagcctaa ccaggaaaat
     3541 ggcatcattg agtactttcg acaaaatgag gttccatctg aagaggatca aggtcctgtc
     3601 tataatgcac ataatgatca gcaggaagaa gaactagcgc cagtggaaga aagtctgttt
     3661 taatttgaga tttatattct gatgaagcac aaaacccgta ttcaatttgt gtaatacggg
     3721 ttttaatccg ctaattatcg tcctgataaa gcagctgcag ctggtgtgta ttcatatcct
     3781 aaatcacgag caacagcctc ataagtgatt ttaccctggt gaacatttag gccattaagt
     3841 aaatgagggt cacttaataa agccagctta acccctttag taacaatact taaaatgaaa
     3901 ggtaatgtgg cattatttaa agcaaatgtt gatgttctgg gtacagcacc aggcatattg
     3961 gccacacaat aatgcactac gttatcaacc acgtaagtag gttcttgatg ggttgtcgca
     4021 cgactcgtct caaaacaccc tccttgatca atagcaacat cgactaaaac ggagccagga
     4081 cgcattgagc tcagcataga gcgagtcact aatttggggg ctgcagcacc tggtaccaat
     4141 acagcaccaa tcactaaatc agcgctggat acgtattttt ctatagaatc tgcagttgag
     4201 taaactgtat tgagtttaga accaaactga aaatccaatt catttaaacg ttgcagtgaa
     4261 cgatctaaca caataacacg agcttccatc cccatcgcca tgcgaacagc atttgttcct
     4321 acaactccac cgccaataac tacgacattg gctgcagcaa caccaggtac accacctagc
     4381 aatataccgc tacccccttg agccatctcc aagcaatgtg caccggcctg gattgccata
     4441 cgaccagcca cttgggacat gggagtaagt aaaggtaacc cgccatcatc ctgtgtcaca
     4501 gtttcataag ctatcgctgt aaccccggat tcttttaata aacgcgcttg atgaggatca
     4561 ggagctaaat gtaaataagt gaataatgtt tgcccttcac gtaaccgttt acattcaata
     4621 ggctggggct cttttacttt gactattaat tcggcattgg caaatacgtc atcagcagta
     4681 tcaaccactt cagccccggc atttctataa tcatcatccg atatgccaat accaagccca
     4741 gcccccttct ctaccaatac atcacttcct gccctaataa tttctctaac actcgaggga
     4801 acaagtccta ctctgttttc gtgcgatttg atttcttttg gtacccctac taacatattg
     4861 ttacctctta tcgattcttt aattgttcta ttaattgtgg aactaattca aacagatcac
     4921 ctactaaccc ataagtcgca atttgaaaaa taggtgcatc tgcgtcttta ttaattgcaa
     4981 caataacctt ggaatccttc atacctgcca aatgttgaac tgctccagaa atacccacgg
     5041 caacataaag cataggagcc actaccttcc cggtttgacc tacctgataa tcattaggaa
     5101 caaaaccggc atcaactgca gctctggatg cccctactgc tgcgcccaaa gtatcagcta
     5161 attcttcgat taatttaaat ttttctgcat tttgtagccc tctaccgcca gaaacaacta
     5221 ttttggcact acctaaatcc ggcctctcgg atttacttaa ttcatggcgc acaaattgac
     5281 ttccttgggc ttgaaattcc ttatcaatac tttctatgca acatacttcc tggcttgatg
     5341 tgatgggatc aaaagctgtc gttctaatag tcattacttt taatgaatcc aaaacacgca
     5401 cagtctctat ggcattacca gcataaacag gatgttcaaa tgtgtctgaa ttaattatct
     5461 tgctcacatc agaaatctgg gtcacatcaa gttgtgcagc aactctgggc aatatatttt
     5521 tcccataagt actggaagga gctaaaatag cactgaatga attcgcaaaa gacaccacca
     5581 aatcgctaac tgcttcagcc aattgatgtt cgtaacaagg attatctaca tgcaaaacag
     5641 catgagctcc ttccagactg gctgcctcct cggctactga acggcattga tatccaacca
     5701 ctagtaatgt aattttatca ccgagttcta aagcagcaga cagtgtattg cgggttgctg
     5761 gatgtaactg ctgattatta tgttcaacta aaactaaagt gctcatctca tctccttaat
     5821 taaagcactt tggcttcatg ttgtaattta tccaacaatg cagccacaga atcaagtatg
     5881 acaccaccac ttctcgttgc cggggcatta acctttaata tttcaacgtg ttgtttaaga
     5941 gataagccaa gcgaatccag atcaatgata tcaaggggct ttcgtttggc cttcataata
     6001 ttcggcaagc tggcatatct gggctcattt aaacgaagat cggtactgat tactgcaggc
     6061 agctgtacct ttagtgtttc caaaccacca tcaatttctc tcaccacttc aagattatta
     6121 ttatcgggaa tgatcttcga cgcattggta gcctgtggcc aattcaataa tgcagccaac
     6181 atttgaggag tttgattgtt gtcaccatct atcgattgtt tgcccatcaa aaccaaatcc
     6241 ggtttttcat tagctacgat attatttaat atcttggcta tattaaggct ctcgaatgat
     6301 ttttctgttc gaaccagaat ggccttatcc gcgcccaaag ctaaagcgtg ccttaaagtt
     6361 tcctgagagc tatccgcacc aatgcttaca gcaataactt ccgttgccca atttttttct
     6421 cgcaaacgca atgcctcttc aatggctatt tcatcaaatg ggttcattgc cattttgata
     6481 ttttgagttt caactcctga attatccgat ttcactcgaa ttttgacata agggtcaatc
     6541 acacgtttta cagccacgag tattttcatg gttcctcact ggaatatcaa atcagtctgc
     6601 atcttgccag agataggggt ataaattcaa gaggttgatt ttaattagaa cataaagcta
     6661 atttattcta aatgagctat gttaaagaaa acctttttct attctcacct gccttataat
     6721 ctggtccggg ttggaaaact ctactcatgc aaaaagaaaa aacatagggc aaaataatgc
     6781 ccttaagcac aattaacagt gcaaacaatg gttatatttt gtcattcacg cataggtaag
     6841 gaagctgttt ttatagcaaa ctgacgatcg agatttgcaa tagcttcact catacacacc
     6901 aatcacacac ataatccgtt atttaaccaa tgtgtgattt tgccctgaga ataagcatga
     6961 gatacttgca acagctatta agtcccatta taaaccacca attgcataga atccttgtct
     7021 tgtagcagag agataacttg ttctgcttct tttacggctt ttgctgtatt gcttgtaaaa
     7081 accttagtat ccttctttat cttatccagt tctttttgag cttctaccaa tttctcctcc
     7141 aaaggactag gaagagaatc cagtactctc atcttgaata aaaatgcctc tacttttaaa
     7201 cgtttctctc gacctatcct caagtcacca aaaaattgct gcgcgtttaa accaagataa
     7261 gattcaagag gagcagaaat gacttttaac tccggtgtct tgaattgcat cccttcacca
     7321 gtattatggc tagccaagat taaatctcta cataaattgg cagctgtatc agactctgat
     7381 ttttccttag tctcaggagc gggtaacata ggttttaaaa tcgcagagcc gatttgtaaa
     7441 ctcacatcaa gaaattggcg cgaagcccaa aaagtcattt caagcaggcg aactaataga
     7501 gtagttgatg cagttgcagt aataatactg atttcttgca acgtgcagcc tatttttttt
     7561 aattgactgg attcagtgct ctccatgtac tgccctcctt tcaataaaat aaaataaagc
     7621 gcatagatag caaacaattc ttctgcaaaa agaaaagtgc catagctggc aaatactact
     7681 ggaataaatc gaagtaaaac tctgagtcct ctaggtttta ttttgccagt atcatcaaaa
     7741 aaaccactgg attgttctaa taaattaatt aaactgtcac aaaagctctg gccagaatag
     7801 gtagttgcct tggatcttaa attttccagc cattgtttaa aatcatcctt gcttttgata
     7861 tctcctaaac tactatcttt gaatttttca ggtgaaatta atttctcata gcacaagcca
     7921 agtatcttga agcatgctcc attattcttg ctctgagata aattattctc tagccaaacc
     7981 atttctggcg aaaatcctga agcatgatcg ggatgaaagc agagagttag tcttttatag
     8041 tttttaacaa ttttttcttt ttctggccca tctatcaata attgctggtt aataaatctt
     8101 gcaaaatctc tttctaat
//
LOCUS       DBNCWK010000131         8102 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.131, whole genome shotgun sequence.
ACCESSION   DBNCWK010000131 DBNCWK010000000
VERSION     DBNCWK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.131"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..1278)
                     /locus_tag="AC06IO_002425"
     CDS             complement(<1..1278)
                     /locus_tag="AC06IO_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HIO9875597.1"
                     /translation="MNQSLSPQQSPGFLFIAVSMITCLILLINVSFKIILLGGLVFAI
                     NSLICPLIAALFLFALRNCTFKEQRHLLNICLMTLYMFCIGVYVLVNLPAAEYMHDNP
                     AYQVIFEEIPKKFFATTIAFALSFYLPHLLFCTKNDRVLSTPKQCVLLALLGGVSFFC
                     LDFYLLFSAPHAHSFKQIFIDSFMISSLLLLIIGVVYLTFLLNDKQHKGKALIETTEL
                     TLFPIYQYLICFAVTVMLVCLACEYRIIAINKDLVLSASCIFFPITIIISSIVGELWG
                     YQANLKLALALLAAQFIFDMLLMGSVALPSPPFFNLNPFYHYIMPRRLSAASLSLFVT
                     FLGNAMLLHYLKYSKWKINRSLRILIANISVNSLLCLVDYSLLYGGIYPYEQIINLAV
                     SVWQYKLLTALIALPVILWMCKILERNYSLVVQT"
     gene            1548..2612
                     /gene="corA"
                     /locus_tag="AC06IO_002426"
     CDS             1548..2612
                     /gene="corA"
                     /locus_tag="AC06IO_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIO9875598.1"
                     /translation="MNTHKTKASAKAGLLPGTAVYIGEHPPQPTQITIHIYDKKNYKR
                     LDSFDTRQVKEAYEAGHNIWIDISGLADIEKITKICSEYNIHPLVIEDLLNTRQRPKL
                     DIVDDYIFVVFKLLQSPPNHLSYNTEQFCLIIKQKLLLTFRESGKYNLSSIYQRLNGE
                     HSLTREYGSDYLTYLIMDYIVDDYFNFVETSAQLLETLEDQLIKKPETIKLQTLYTIK
                     RRTITLRKIIPPLRDIVHLLLTDDGGLINKKYNLYFRDLYDHCIRLVESIDLHREMSA
                     SMLDIYLSTINNRMNESMKILTLFASIFIPLSFIAGVYGMNFKFMPELDWRYGYPMIL
                     VTMVLLAILMLYFFKRKKLF"
     gene            complement(2725..4476)
                     /locus_tag="AC06IO_002427"
     CDS             complement(2725..4476)
                     /locus_tag="AC06IO_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2879 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875599.1"
                     /translation="MLQESIMKWFSSVVNGVKTIGSSLWEGSKWVWNNASLSKITTGV
                     FSYTANTTFQVLEQAIALRDAVPTLVNNPQAKKIAYGMGYILVNDVLPLVALNFANNT
                     AQNYFGEGYDEDASWYAPYSLFLSGLALTNYLVKAYTWRQGVQTVAHVAVLDSLGPSS
                     FNSNKKSLPPSICDELDCNYKRKIKGWGREPLVLMGNDALTYGVSLIPYVGEPLSQVL
                     RVYFNGRYIIRLTTPELCERHKYLAMKPESVLGFGLTFEATSMLMDKALESTVGMPPF
                     LYYRALRHLLLLMHINVAAHMELPKSDLKDKASSFDPFNVYERVNRFVADVIFSGLIK
                     RIPIDFKPDKDAEPLIPLSPALKFGTMVLNMDLEKEKKTAPGFFSKAIDKAYVLVLPP
                     VFRGANGLINDPIVFMYWPTIRSGAISAVEFVEGVGKTKTMATLAWAPKTVAAAINLK
                     FGIPKKVTRFVLMLSKEEDFWNLATAFKLWFERHNIKGEPKLITGSNLALNGVTPLQP
                     TPVSVEKEPIVSPHLLITEKDTKEVVPAKSLIPVRSQPQTGVVVTISPDSLFSTRRRR
                     VNPDQEKVAITELSMTK"
     gene            complement(4717..5352)
                     /gene="nth"
                     /locus_tag="AC06IO_002428"
     CDS             complement(4717..5352)
                     /gene="nth"
                     /locus_tag="AC06IO_002428"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIO9875600.1"
                     /translation="MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATD
                     VSVNKATAKLFPAANTPQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCEILIDQHEG
                     QVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVE
                     QELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL"
     gene            complement(5345..5959)
                     /locus_tag="AC06IO_002429"
     CDS             complement(5345..5959)
                     /locus_tag="AC06IO_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIO9875601.1"
                     /translation="MASVKEIDALLPQTQCGECGYAGCLPYAEALTQGAALINKCPPG
                     GVETVNALGKLLHVDASPYLKEAIDNTRPPSVAAIKEDECIGCTKCIKACPVDAIIGS
                     SKLMHAIITHECTGCGLCVDPCPVDCIEMVSLSAAGYDKDLARQRYNAKQMRQLRDEH
                     EKQQIYRERKKLSAHAQDHTQDVQAKQNYILEALARVKAKKTYE"
     gene            complement(5959..7971)
                     /gene="metG"
                     /locus_tag="AC06IO_002430"
     CDS             complement(5959..7971)
                     /gene="metG"
                     /locus_tag="AC06IO_002430"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIO9875602.1"
                     /translation="MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQ
                     CISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPEN
                     QALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
                     CGATYSPTDLINPVSVISGASPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKL
                     SEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFD
                     EFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKS
                     RGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIAS
                     RCAGFINKRFDNRLSSELNDPKLHNDLLSARESVIDAFVSRDYARAIRQIMDCADKVN
                     QYIDANKPWVLAKDESKLNEVHAICTMGINLFRILITYLKPVLPMMAKASEEFLNSEP
                     LHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLAQSKESLMTTPTKENTPVEDSNLI
                     SIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRL
                     TVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPDAGALPGMKVK"
BASE COUNT         2574 a         1686 c         1467 g         2375 t
ORIGIN      
        1 tgtttgcaca actaatgaat aatttctttc taaaatcttg cacatccata aaatgaccgg
       61 gagagcaatt aacgctgtca atagtttgta ttgccaaaca ctcaccgcta aattaataat
      121 ttgttcgtaa ggataaatac cgccataaag caaactgtag tcaaccaggc ataataacga
      181 gttgactgat atattagcga ttaatattcg caaggatcgg ttaattttcc attttgaata
      241 ctttaaatag tgcaataaca ttgcattacc aagaaaagtg acaaacaatg acaacgatgc
      301 agcggataac ctgcgtggca tgatgtaatg ataaaaagga ttcaaattaa aaaaaggagg
      361 agaaggtagt gcaacactcc ccatcaataa catgtcaaaa ataaattgtg ccgcaagaag
      421 agccaatgct aatttcaagt tggcctggta accccacaat tcgccaacta tagaactgat
      481 aatgatagta attggaaaga aaatacagct ggccgataaa accaaatctt tattgatcgc
      541 gatgattcga tactcacagg ccaaacacac caacatcacg gtcacagcga agcaaatcaa
      601 atattgataa attggaaaaa gcgttagttc tgtggtttca attaatgctt tgcccttatg
      661 ttgtttgtcg ttcaataaaa atgttaaata aactacgcca ataattagga gcaaaagaga
      721 ggagatcatg aatgaatcga tgaaaatttg cttgaaactg tgagcatgtg gagcagaaaa
      781 taatagataa aaatccaggc aaaaaaaact tactcccccc agcaaagcta ataacacgca
      841 ttgctttgga gttgataaaa ccctgtcatt tttagtgcaa aacaataaat gaggcaaata
      901 gaaactgagt gcaaaagcaa tagttgtagc aaaaaatttc ttaggtattt cttcaaaaat
      961 aacttgatag gctggattat catgcatgta ttcagcagca ggtaaattga cgagcacata
     1021 aaccccaata caaaacatat ataaagtcat caaacaaata ttaagtaaat ggcgttgctc
     1081 tttgaaggta caatttctca atgcgaataa aaataacgct gcaataaggg ggcaaatcaa
     1141 actgtttatt gcaaatacca gccctccgag tagaataatt ttaaaagaaa cgttaattaa
     1201 taatatcagg caagttatca tactcactgc gatgaataga aacccggggg attgttgggg
     1261 tgataacgat tgattcatac gacaagagct tccatagtat ataacaactc attctgcctt
     1321 attgatagag actgactata gggagttaat tctttatgat ttacatggaa gtattaacta
     1381 catccctata agcttcaaat aataattgcc ctttattaag ctaatcaggt cgcaaatata
     1441 gcattatagc cctttgcata gcaagataaa caagagtcgt caactgtcga cttaaacgat
     1501 gactaaatga taagttaata agaaaataaa taagctgaga taacttcatg aatactcata
     1561 aaacaaaggc ttcagcaaaa gcgggactat taccgggaac tgccgtatat ataggcgaac
     1621 acccaccgca accaactcaa ataacgatac atatttatga caaaaagaat tataagcgtt
     1681 tagattcctt cgatacccga caagtgaaag aggcctatga ggctggacat aatatttgga
     1741 tagatatttc aggtcttgca gacatcgaga aaattactaa aatttgctca gaatacaaca
     1801 tacacccctt agttatcgag gatttgctta ataccagaca aagacccaaa ctggatattg
     1861 tagacgatta tatttttgtt gtttttaaat tattacaatc ccctcccaat catttatctt
     1921 ataatacaga acagttctgt ctaatcatca agcaaaaact tttactcaca ttcagagagt
     1981 ctggaaaata taatttatcc tccatttacc aacgtcttaa tggagagcac tcgttaacca
     2041 gggaatatgg aagcgattat ttgacctatc tgatcatgga ctacattgtt gatgattatt
     2101 tcaactttgt cgaaacttca gcccaactgc ttgaaacctt ggaagatcaa ttaattaaaa
     2161 aaccagaaac gattaagtta caaactttat acaccatcaa aagaagaacc attaccctca
     2221 gaaaaatcat tcctcctttg agagatattg tccatttgtt gcttacagac gatggggggt
     2281 taatcaataa aaagtataat ctttacttcc gggacttata tgatcattgc attcgcctgg
     2341 tagaatcgat cgatttgcat agagaaatgt cggcaagtat gttagacata tacctgtcta
     2401 ctataaataa tcgcatgaat gaatccatga aaattctgac cttgtttgcc agtattttta
     2461 ttcctttaag ttttattgcc ggggtttatg gaatgaattt taaatttatg cctgagctgg
     2521 attggcgata cggttaccct atgattctgg ttaccatggt acttctggca atactcatgc
     2581 tgtatttttt taaacgaaaa aaattatttt aagtggcgtc aagcccccca tttacaacct
     2641 gcataatatc atcgatagaa ctgggtaggg tctttaccgg gcaacacact tctgttgccc
     2701 tcttgtcaga aaagcctcta aaatttattt agtcatgctc agttctgtta tcgcaacttt
     2761 ttcttgatct ggattaaccc gacgtctcct agttgaaaat aaactatcag ggctaatcgt
     2821 cactacaact ccagtctgag gttgggatcg aacaggaatc aagctttttg ctggcactac
     2881 ttccttagta tctttttccg taattagtag atggggagaa actatgggtt ccttttcaac
     2941 agatacggga gtaggctgaa gaggggtcac cccattcaaa gccagattgg aacctgtaat
     3001 taattttggt tcacctttga tattgtgtct ctcaaaccac aacttgaatg ctgtagccag
     3061 attccagaag tcttcttctt tgcttaacat taaaacaaag cgtgttactt ttttagggat
     3121 tccaaatttg agattgatgg cggctgctac agtttttggt gcccatgcca aagtagccat
     3181 cgttttggtt ttccccactc cttctacaaa ttcaacagca gaaatagctc ctgagcgaat
     3241 agtaggccag tacataaaca caattggatc gttgattaag ccattagcac cgcgaaaaac
     3301 aggtggtaaa accaaaacat acgctttatc tatggctttg ctaaaaaatc cgggagctgt
     3361 tttcttttcc ttctccaaat ccatattcaa caccatagtg ccaaatttta gagcgggaga
     3421 taatggaatt aacggctcag catctttatc cggtttgaaa tcaatgggta tccttttaat
     3481 taaacctgaa aatatgacat ccgctacaaa gcgattgacg cgttcataaa cattaaacgg
     3541 atcaaaagac gaggctttgt ctttcaaatc agatttaggc aactccatat gtgcagcaac
     3601 attaatatgc atcagcagca ataaatggcg cagagccctg taatataaaa atggaggcat
     3661 gcccacagtg gattccagag ccttgtccat taacatactt gtcgcttcaa aggttaaacc
     3721 aaatcctaaa acagactcgg gtttcatggc caaatattta tggcgctcac acagctccgg
     3781 tgtagttaaa cgaataatat agcggccatt gaaatacact cttaatactt gcgagagagg
     3841 ctcacctaca taaggaatta aactaacccc ataagtcagt gcatcattac ccattaaaac
     3901 aagaggttct cttccccaac ctttaatttt acgcttatag ttacaatcca attcatcgca
     3961 gattgaagga ggaagtgatt tcttatttga attaaatgag gaagggccta atgagtcaag
     4021 aaccgctaca tgtgctacag tttgaactcc ttgccgccag gtgtatgctt tgaccagata
     4081 attcgttaga gctaatcccg ataaaaaaag agaataaggc gcataccatg aagcatcctc
     4141 atcataaccc tctccgaaat aattttgtgc cgtattgttt gcaaaattta aagcaactaa
     4201 cggtaaaaca tcgttgacaa gaatgtaacc cattccatag gcaattttct tcgcctgagg
     4261 attgttgacc aaggtaggga cagcatcacg taaggctatt gcctgctcta acacttgaaa
     4321 agttgtattt gcggtatagc taaatacacc agtcgtgatt tttgacaatg aggcgttgtt
     4381 ccaaacccat tttgaacctt cccacaggct tgacccaatt gttttgactc catttaccac
     4441 cgatgaaaac cacttcataa tgctctcctg caacagcgga atttcatttt atgagtaaaa
     4501 attgggaaat accagaggga aagagcactt ttcttgaact ttattgaaat tttttataag
     4561 agctgatggt caattgaact tttgtgtttt aactgtacaa aagctaatct caacccggat
     4621 gatttgatag aatgaatttg aaatacaaca gccagtcatt tttatgaggc atagattgtt
     4681 cattatcgtt tgattttcct tcattatggc tttgtcttaa agtaaattct tgtctttgta
     4741 ttcgcaaaga tcttcaatga tgcaagtctt gcaatgtggt ttacgtgctg tacagacata
     4801 acggccatgt aatattaacc agtgatgagc gtcatgtaaa aactcacggg gcactttttt
     4861 taataactcc tgctcaactg ctagcggtgt attacccttc gctatgccaa tccggttagc
     4921 cactctgaaa atatgggtgt cgaccgctat ggttggctga ccaaatgctg tattcaaaac
     4981 cacatttgca gtttttcggc caacaccagg caatgcttcc agcgcctctc tctggccagg
     5041 aacttgtccc tcatgctgat caataagtat ttcacaggtt ttcatgatat tttgtgcttt
     5101 gctattataa agacctatgg acttaatgta atcttttaat ctatctaatc ccaaatccag
     5161 caaggcttgt ggggtattgg ctgccgggaa tagtttggct gtcgctttgt tcacactaac
     5221 gtctgttgcc tgggctgaaa gcatgacggc aattaataac tcaaaggggg tactatattg
     5281 caactccgtt gtaggatgag ggttttgttc ttgaaaacgt ttgaaaattt cttgtgattt
     5341 ttgtttattc atatgtcttt tttgctttca cccgagccaa agcctcaaga atataatttt
     5401 gttttgcctg tacgtcttga gtatgatctt gcgcatgggc tgaaagtttc tttctttctc
     5461 tgtatatttg ttgtttttca tgctcatccc gcaactggcg catttgtttg gcattataac
     5521 gctggcgggc caaatcctta tcatatccag ccgcagacaa actaaccatc tcaatgcagt
     5581 caacaggaca aggatccaca cacaagccgc atccagtgca ctcatgagta ataatggcat
     5641 gcattaattt actactacca ataattgcat caacaggaca ggccttaatg catttagtac
     5701 aaccaatgca ttcatcctct ttgatagcag caactgaagg aggccgagta ttatctattg
     5761 cttctttcaa atatggagat gcatcaacat gcagtaattt tcccaaagca tttacagttt
     5821 caacaccacc aggagggcat ttattgataa gggcagctcc ttgggtaagc gcttcagcat
     5881 aaggcaaaca acctgcatat ccgcattccc cacactgggt ttgcggcaat agggcatcaa
     5941 tttctttaac agaggccatt acttcacctt catgccagga agtgctccag catcaggctg
     6001 taataaataa attccctgtc catcccctgc cgccaatacc atgccttcag aaacaccgaa
     6061 acgcatagtg cggggctcca aattagcaac cattacagtc aaacgcccaa tcaactcttc
     6121 agcatcataa gcctttttta tgccggcaaa aacctgcttt tgagcatcac ccaagtctaa
     6181 aatcaagcgc attagtttat ctgcgccttc cacaggttct gcattgacaa tttttgcaat
     6241 ccgtaaatca actttggcaa aatcctcaat actgattaag ttagagtcct caactggcgt
     6301 attttctttt gttggggtag tcatcaaaga ttccttgctt tgagccaaca tcgcttcaat
     6361 tttttctttt tctactctga ccatcaaagg cttaaaagta ttgatacggt ggtttagcaa
     6421 aggcttatcg atagaacccc agtgtaaagg ctcagaattt aaaaattctt ctgaagcttt
     6481 ggccatcatt ggcaagactg gttttaaata ggttattaaa atgcggaata aattgattcc
     6541 cattgtacat atggcatgaa cttcatttag tttactttca tctttagcca atacccaagg
     6601 tttattggca tcgatgtatt gattgacttt gtcagcacaa tccatgattt gtcttattgc
     6661 tcgcgcatag tctcttgaaa caaatgcgtc aatcacggac tctcttgcgc tcagcaaatc
     6721 gttgtgtaat tttggatcat tcaactctga gctcaaccga ttatcaaaac gcttattaat
     6781 aaatcccgcg catcggcttg caatattcac taccttccca accagatccg cattcaccct
     6841 attgacaaaa tcgtcaaaat tcaaatccaa atcgtctact cgaccattca atttggcagc
     6901 gaaataataa cgcagatatt caggatgcaa gtgagccaga taagcgcgtg cttctataaa
     6961 agtaccgcgc gatttggaca ttttctgccc ctcaaccgtt aaaaacccat gggtatacac
     7021 agcagtaggt gttctgaaac cgctggcagc taacatagca ggccagaaca aagcatgaaa
     7081 atacacaata tctttgccta caaaatgata taattctgtt tttgaagcct tatcccaaaa
     7141 ctcatcaaaa gaaacgccac gctcatcgca atacttttta aagcttgcca tatagccgat
     7201 gggagcatct aaccaaacgt aaaagtattt atccggaaca ccgggaatag ggaaaccaaa
     7261 atagggagca tcgcgagaaa tatcccattg ctttaaaccc gcctcaaacc attcgctgag
     7321 tttattcgtt acttcggttt gtaaatgccc ctttcttgtc caatccttaa gtaactcttc
     7381 gtaacgcggc aaatcaaaaa aataatgctc tgattccttt tctacagggc tggctcccga
     7441 tataacagaa acagggttaa tcaaatccgt cggcgaataa gtggcgccac aaacctcgca
     7501 attatcgcca tactgatcga cagcagcaca tttggggcaa gttcccttca catacctgtc
     7561 cggcaaaaac atttgcttta cgggatcata aaactggcga attgttttct tgactatatc
     7621 accgcctgcc tgcaaacgtt caaatatggt agtcgccaat gcctgatttt caggtgaatg
     7681 agtagtgtgg taataatcat aatcaatggc gaaggcttta aaatcctttt catgacttaa
     7741 cttaatttcg gcggttaaag cttccggagt aattcccagc tgctctgctt tcagcataat
     7801 gggtgttcca tgagcgtcat ccccacaaac gctaatacat tgaataccca acattttgtg
     7861 agtacggacc caaatatccg tttgaatatg ttcaaccaaa tgtcccagat gcaaatgccc
     7921 attagcataa ggcagggcac tggtcactaa catttttcgt tcactagtca tagttataga
     7981 atactcatca cctataataa agcgactaat tataacgcaa agacaatgga aatgctgcaa
     8041 tagaaaacag gtttttattg gtagttattt atgaagtgtt aacttttacc gagatgatcc
     8101 ga
//
LOCUS       DBNCWK010000132         8065 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.132, whole genome shotgun sequence.
ACCESSION   DBNCWK010000132 DBNCWK010000000
VERSION     DBNCWK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.132"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            140..1804
                     /gene="hutU"
                     /locus_tag="AC06IO_002431"
     CDS             140..1804
                     /gene="hutU"
                     /locus_tag="AC06IO_002431"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIO9875603.1"
                     /translation="MNKDKNKRVISAPRGTEIQAKNWLTEAALRMICNNLDPNVAEDP
                     DSLIVYGGLGKAARNWECFDEIVHVLKLLNNDQTLLIQSGKPVGVFTTHEEAPRILIA
                     NSNLVPRWATWEHFNELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAAAKKHYQ
                     GDLSGRWILTAGLGGMGGAQPLAGTMAGASVLAVECDRQRIEKRLQTKYLDRYTDNLS
                     EALDWINESCRRKKPVSVAVLGNAAEIFPQLVKLGVQPSLVTDQTSAHDPLNGYLPLG
                     WTLEQAVEMRKKSPQEVVDAAKKSMAVQVHAMLEFHNRGIPVFDYGNNIRQMAFEAGE
                     KNAFSFEGFVPAYIRPLFCEGIGPFRWVALSGDPEDIYATDERVKQLIPDDPHLHHWL
                     DMAREKISFQGLPARICWVGLKDRARLALAFNEMVKNKEVKAPIVIGRDHLDSGSVAS
                     PNRETEGMLDGSDAVSDWPLLNALLNCASGATWVSIHHGGGVGMGFSQHAGVVIVADG
                     TEKAAKRLARVLHNDPATGVMRHADAGYQIAKQCAKENSLWLPMES"
     gene            1818..3338
                     /gene="hutH"
                     /locus_tag="AC06IO_002432"
     CDS             1818..3338
                     /gene="hutH"
                     /locus_tag="AC06IO_002432"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIO9875604.1"
                     /translation="MSEHFILQPGQLSLLSIKQILDEGQSCVLAESAFELINASHQTV
                     KKVIDEKKTVYGINTGFGSLANQTISADCLKELQRNIVLSHACGTGKLLPDDVVALIL
                     LLKINNLSQGYSGVRLELINALIALFNHKVYPCIPSKGSVGASGDLVPLAHLSLPLLG
                     EGEVRHQGQLISAEEGLKLAGLKPLELEAKEGLALLNGLQVSTALALSALFISETLFE
                     TAIISGSLSVDAASGSDVPFDDRIHQIRGHQAQISAASMYRNLLAGSQIRESHRHCNR
                     VQDPYSLRCQPQIMGAVLHQMQFVGQTLQVEANAISDNPLVFAEQGDILSGGNFHGEI
                     IAMAADNLALALSEIGGSAERRIALLIDKNFSGLPAFLVRESGLNSGFMIAHVTAASC
                     ASDNKALAHPHSVDSLPTSANQEDHVSMATSAARRLHEMIDNTSTILAIELLAACQGL
                     EFHKPLKTSPQLDKIYQSVRSVVKEYDKDRYFAPDIEKIKKKILDKEFSLLTLTNE"
     gene            3352..4740
                     /locus_tag="AC06IO_002433"
     CDS             3352..4740
                     /locus_tag="AC06IO_002433"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006081 - aldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HIO9875605.1"
                     /translation="MELMAEFLTLHQQHKNNPYPSLSERKKLLIAIKKILQTEAYTLA
                     EAINKDFTHRPVEETLFLEVFPTIKAINFCLKNMKKWMRKRRRNVSWLFMPAQAYVIP
                     QPLGVVGILVPWNYPVYLALVPAIYALASGNRVMIKMSELSPHTGDTLQKLVHASGIN
                     HSISVINGDIELSKHFASLPFGHLMFTGSTNVGKLVMKAASNNLTPVTLELGGKSPAI
                     LSPTMNPAYFKRLFMGKLLNAAQTCIAPDYVLIPKGWEERVETEFKKFINTFYPDLMS
                     NEQYSSIISESHKKRLLDLVEDARSKGARVVEFGHLIPNSSKLPVFLLFDITNDMLVM
                     KEEIFGPVLPVLTYNSIIDAVDFINSFPNPLALYYFGEDKNEIKIIQTTTLSGALTIN
                     ETLMHIAIDDLPFGGVGHSGMGHYHGKEGFDTFSKLKPVLVQRFISTVSWLYPPYGAF
                     MRMFLAWVGGIK"
     gene            4752..5561
                     /locus_tag="AC06IO_002434"
     CDS             4752..5561
                     /locus_tag="AC06IO_002434"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIO9875606.1"
                     /translation="MSKVIFITGGSRGIGREIALKFAAKDKANIVIAAKTAEPHPKLE
                     GTIYSVAKEIEELGGQALPLMVDVRDEQQIQNAITKTIETFGRLDVLVNNASAINLTD
                     TLNTPIRRYDLMQSVNVRSTFACSQAAIPYLLKSENPHILTLSPPLNMDKTWFAPHLA
                     YTISKYGMSMCTLGLAEEFKEAGIAINSLWPKTTIATDAIRVHFPSELYMASRKPLIM
                     ADAAYWIINQPSKSITGNFFIDEDVLKNSGVTDFSCYAMNPKVQLCPDLFL"
     gene            5558..5989
                     /gene="rnhA"
                     /locus_tag="AC06IO_002435"
     CDS             5558..5989
                     /gene="rnhA"
                     /locus_tag="AC06IO_002435"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIO9875607.1"
                     /translation="MKVEIYTDGACKGNPGPGGWGVLLRYNGREKTLHGGEAQTTNNR
                     MELMAAIKGLEALKRPCEVDLYTDSQYLQQGMKEWIKTWKRNGWRNSKKELVKNAELW
                     KSLDNLASIHNINWHWVKGHSGHLENDLVDALANLGIEELS"
     gene            5994..6695
                     /gene="dnaQ"
                     /locus_tag="AC06IO_002436"
     CDS             5994..6695
                     /gene="dnaQ"
                     /locus_tag="AC06IO_002436"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIO9875608.1"
                     /translation="MRQVILDTETTGIGPEQGHRVIEIGCIELIDRKLTGKHFHIYLN
                     PQREVDEGAFRVHGISTEFLQDKPLFQDIVTEFIQFVEGSELIIHNAPFDVGFLNSEL
                     KHVKWNKTLEDYCRILDTLILAREKHPGQRNSLDALCKRYEIDHFNRELHGALLDAEI
                     LAYVYLAMTGGQSSLFTEVESSFSQSKIKTQEIKSLSLKKPVIMKANEEELMQHQSFV
                     EFISKKSGINYWEEV"
     gene            complement(6903..7280)
                     /locus_tag="AC06IO_002437"
     CDS             complement(6903..7280)
                     /locus_tag="AC06IO_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875609.1"
                     /translation="MSRKLLIATTLVLSTSLFPLISNAEDTANPNEMTKDAWLNSMTP
                     LLPDLICKGFIQDPDLKKRFDEIKMTYEQCVTLIPESTKKCQDELYASMPDKINSETA
                     GTWGRSLGECIGKDFAEKHLIPK"
     gene            7572..8045
                     /locus_tag="AC06IO_002438"
     CDS             7572..8045
                     /locus_tag="AC06IO_002438"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIO9875610.1"
                     /translation="MEKSNFRLNFLSQILLFTLGVFLFCNGYAAGNTFIFNPNTLTWK
                     AISANGKVIRTGRGSGGKHYCSDVRRSCKTPSGVFRVISKGGPACRSSRYPLGKGGAP
                     MPYCMFFSKLYAIHGSPDVPNYNASHGCVRVKPHDARWLSQNFIKVGTKVIIKSY"
BASE COUNT         2441 a         1353 c         1771 g         2500 t
ORIGIN      
        1 tttatctggt ttttctcaac tcattagatt atctcattcc cggaggtagt tgactggtgt
       61 atgatgctaa aaaatttttt aatcgctatt tgatgggaaa taataactga agttttcata
      121 gcaataagga gtattcttga tgaacaagga caagaataaa cgtgttattt ctgcccctag
      181 aggtaccgaa attcaagcta aaaattggtt aactgaggcg gcattaagga tgatttgcaa
      241 taatttggat cccaatgttg cagaggatcc cgattctttg attgtttatg gtggattggg
      301 caaggcagcc aggaactggg aatgttttga tgaaattgtt catgtattga aattattaaa
      361 taatgatcag actttactca ttcagtccgg aaaacctgtc ggtgtattta ctacccatga
      421 agaggctccc agaatactaa ttgccaattc taatttggta ccgcgctggg ctacttggga
      481 gcattttaac gaactggata agaaaggcct gatgatgtat ggccagatga ccgccggcag
      541 ctggatttat attggttctc aaggcattgt gcaaggaact tatgagacat ttgttgcggc
      601 agctaaaaaa cattatcagg gtgatttgtc cgggcgttgg attttaacag cgggtttagg
      661 gggaatgggt ggagctcaac ccttggctgg aactatggct ggtgccagcg ttctggctgt
      721 agaatgtgac agacaaagga ttgagaaacg tttacagaca aagtatctgg atcggtacac
      781 tgataattta agtgaagcat tagactggat taatgaatcc tgtcgccgta aaaagccagt
      841 ttcagtagct gttttgggaa atgctgccga aatatttcct caactggtta agttgggggt
      901 gcagccatct ctggttaccg atcaaaccag cgctcatgat ccgctcaatg gctatttgcc
      961 attaggatgg acattagaac aagctgttga aatgagaaaa aaatcaccac aagaggttgt
     1021 tgatgcggcc aagaaatcga tggctgttca agtgcatgca atgcttgaat ttcataatag
     1081 aggcattcct gtatttgatt atggcaataa catcagacaa atggcatttg aagcgggaga
     1141 aaaaaacgct ttttcctttg agggatttgt acctgcttat atcaggccat tattttgcga
     1201 aggaattggt ccatttcgat gggttgcatt gtcaggtgat ccagaagata tttatgcaac
     1261 ggatgagaga gtaaagcagt taattcctga cgatcctcat ttgcatcatt ggttagatat
     1321 ggctagggaa aaaatttcat ttcaaggatt accagcaagg atatgttggg tagggttaaa
     1381 ggatagagca cgccttgccc tggctttcaa tgaaatggtt aaaaataaag aagttaaagc
     1441 gcctattgtg attggccgtg accatttgga ttctggttct gtggctagcc ccaatcgtga
     1501 aacagaaggt atgctggatg gcagtgatgc tgtttctgat tggcctttgc ttaatgcgct
     1561 gttaaattgt gccagtgggg ctacttgggt aagcattcac cacggaggag gcgttggtat
     1621 gggcttttcg caacatgctg gcgttgtgat tgttgctgat gggacagaaa aggcagctaa
     1681 acgattagcc cgtgtgctac acaatgatcc tgcgacaggg gtaatgaggc atgctgatgc
     1741 aggttatcaa attgccaagc aatgtgccaa agagaactcg ctttggctac ccatggaatc
     1801 ttaaaaggag cctgtttatg tcagaacatt ttatattgca gcccgggcaa ttatcgctac
     1861 tttctattaa gcaaattttg gatgagggtc aatcttgtgt tctcgcagag agcgcttttg
     1921 aattaataaa tgcttctcat caaactgtta aaaaagtaat tgatgaaaag aaaacagtat
     1981 atggaatcaa tacaggtttt ggttctctgg cgaatcagac catttctgca gattgtttga
     2041 aggagttgca gcgtaatatt gtgctttctc atgcttgtgg aacgggtaaa ttattgccgg
     2101 atgacgtggt agccttgatt ttgcttttga aaattaataa tttgtctcaa gggtattccg
     2161 gagttaggct ggaattaatc aatgcgttga ttgctttgtt taatcataaa gtatatccat
     2221 gcattccttc taagggttcg gtaggtgcgt ccggagattt agtcccgctt gcgcatttgt
     2281 ctttgccttt gttaggggaa ggcgaagtaa gacatcaagg tcagttgatc agtgctgagg
     2341 aaggactaaa actggctgga ttgaaaccgc ttgaattgga agctaaagaa ggattagcac
     2401 tgcttaatgg cttacaggta tctaccgctt tagctttgtc agctctgttt attagtgaga
     2461 cattgtttga aactgcgatt atttccggca gtttatcagt agatgctgcc agtgggagtg
     2521 atgtcccctt tgatgacaga atacaccaaa ttcgaggaca tcaagctcaa atttctgctg
     2581 ccagtatgta cagaaattta ttggcaggta gtcaaattcg agagtcacac aggcattgta
     2641 atcgtgttca ggacccttat tctttaagat gtcagccaca aatcatgggt gcagttcttc
     2701 atcagatgca gtttgttgga caaactctcc aagtagaggc caatgcaatt tccgataatc
     2761 ctctcgtttt tgcagagcag ggggatattt tgtctggtgg aaattttcat ggtgaaatca
     2821 tcgcaatggc tgctgacaat ttagcattgg cattgtccga gataggaggt agtgcagaac
     2881 ggcgaatcgc tttattaatt gataaaaatt ttagtggatt gcctgctttc ctggttagag
     2941 aaagcggctt aaattcaggt tttatgattg cccatgttac tgccgcctcc tgtgccagtg
     3001 ataacaaagc acttgctcat ccgcactccg ttgatagttt gcctacctcg gcaaatcagg
     3061 aagatcatgt ttctatggca actagcgcag cacgtcgact gcatgaaatg atcgataata
     3121 cttcaacaat tcttgctatt gagttattgg cagcttgtca agggctggag tttcataaac
     3181 ctctaaagac atcaccgcaa ttggataaaa tttatcaaag cgtaagaagc gtggttaaag
     3241 agtatgataa agatcgctat tttgcacctg atatagaaaa aattaaaaag aagattttgg
     3301 ataaagaatt ctcattgctc acactcacga acgaatagca ggaaaaatac aatggaattg
     3361 atggcagaat ttctaacttt gcatcaacaa cacaaaaaca atccttatcc ttctttgtct
     3421 gaaagaaaaa aattgctgat tgcaataaaa aaaatcttgc agacagaagc ttacaccttg
     3481 gcagaagcaa taaataagga ctttactcat cgccctgttg aggaaacctt atttttggaa
     3541 gtttttccta cgataaaagc cattaatttt tgtttaaaaa atatgaagaa atggatgaga
     3601 aagagacgaa ggaatgtgtc ttggttgttt atgcctgcac aagcctatgt tattccccaa
     3661 ccccttggag ttgtaggcat cctggttccc tggaattatc ctgtctatct ggctttagtc
     3721 cctgctattt atgccttggc atcgggaaac agggtaatga taaaaatgtc cgagttatct
     3781 ccacatactg gcgatacttt acaaaaattg gtacatgcaa gtggaataaa ccattctatc
     3841 agcgtcatta acggcgatat tgagctatca aaacactttg cttctctgcc ttttggccat
     3901 cttatgttca ctggttcaac taacgtagga aaactggtga tgaaggctgc cagtaataac
     3961 ctgactccag tgacactgga gctaggtggt aaatctccgg caatattatc tcccactatg
     4021 aatccagctt attttaagcg cctatttatg gggaaattat tgaatgccgc tcaaacttgt
     4081 attgcacctg attatgtatt gattcctaaa ggatgggagg agagagttga gacggagttt
     4141 aagaagttta ttaatacctt ttatccagat ttaatgagta atgagcagta ttccagtatc
     4201 atatcagaga gccataaaaa gcgattactt gatctggtcg aagatgcgcg aagcaaaggg
     4261 gcccgtgttg ttgagtttgg tcatttaatt ccgaatagct cgaagctacc tgtgttttta
     4321 ttatttgata taaccaatga tatgcttgtt atgaaagagg aaatttttgg ccctgttctt
     4381 cctgtgttga cttataattc gataattgat gccgtagatt tcattaattc ttttcccaat
     4441 ccgcttgccc tatattattt tggagaagat aagaatgaaa taaagatcat acaaacaaca
     4501 acattatctg gtgctttgac aattaatgaa accttaatgc atattgcgat tgatgatttg
     4561 ccatttggcg gtgttggcca tagtggtatg gggcattatc atggaaaaga agggtttgat
     4621 accttttcaa aattaaaacc ggtattagtc cagcgattta tatctacagt gagctggtta
     4681 tacccaccct atggtgcttt tatgcgtatg tttttggctt gggttggtgg tatcaagtag
     4741 aaggagaaat catgagtaaa gtcattttta ttacgggtgg cagcagagga attggtagag
     4801 aaatcgcttt aaagtttgca gcaaaagata aagctaatat cgttattgca gcgaaaacag
     4861 cagaacctca tcctaaattg gaaggaacta tttattcagt tgcaaaagaa attgaagagt
     4921 taggcggaca agccctgcct ttaatggttg atgtcagaga tgagcaacag atccaaaatg
     4981 cgatcacgaa aaccatagaa acttttggaa gattggatgt attagtcaat aacgcgagcg
     5041 caatcaattt gactgataca ttaaatactc caattaggcg ttatgatctc atgcagagcg
     5101 ttaatgtcag gtcaacgttt gcatgttctc aagcagccat tccatatctt ttaaaaagcg
     5161 agaatccgca tattctgact ttgtctccac ctttaaatat ggataaaacc tggtttgcac
     5221 cgcatctggc ttatactatc agtaaatacg gtatgagcat gtgcacatta ggcctcgctg
     5281 aggaatttaa agaggcggga attgctatta attctttatg gccgaaaact actatagcta
     5341 ctgatgccat aagagttcat tttcccagtg agctgtatat ggcaagcaga aagccactca
     5401 ttatggctga tgctgcatat tggattatca atcaaccatc aaaatcaatc actggaaatt
     5461 tttttattga tgaagacgta ttaaaaaata gtggtgtcac tgatttttct tgctatgcta
     5521 tgaatcctaa agttcaactt tgtcctgatt tatttttatg aaggtagaaa tctatactga
     5581 tggtgcatgt aagggaaatc caggacctgg cggctggggc gtattactcc gatataatgg
     5641 tagagaaaaa actcttcatg gaggagaggc gcaaactacc aataatcgca tggaattaat
     5701 ggcagcaatt aagggcctgg aagcattaaa acgtccttgt gaggtggatt tgtataccga
     5761 ctcccaatat ttgcagcaag gaatgaagga atggatcaaa acatggaaaa gaaatggttg
     5821 gcgtaactcc aagaaagagt tagttaaaaa tgcggaatta tggaaatcat tagataatct
     5881 ggcttctatt cataatatta attggcattg ggttaaaggg cactcaggtc atttggaaaa
     5941 tgatttggtt gatgctttgg caaatttggg tattgaggaa ttatcataga gttatgagac
     6001 aagttatatt agataccgaa acgactggta ttggacctga acagggacat agagtaattg
     6061 aaataggttg tattgagctt atcgatagaa aattaaccgg caagcacttc cacatatacc
     6121 ttaatccaca gcgtgaagtg gatgaagggg catttagagt tcacggtata agtactgaat
     6181 ttttgcaaga taaaccttta ttccaggata ttgtgactga gtttatacaa tttgtggaag
     6241 gttcagaatt aattattcac aatgctccat ttgatgttgg atttctgaat tctgaattaa
     6301 aacatgtgaa atggaataag acattagaag attattgccg gattcttgat actttgattt
     6361 tagccagaga aaaacatcct ggacagcgta atagtttaga tgctttgtgt aaacgttatg
     6421 aaatagatca ttttaatcgt gaactgcacg gagctttgct tgatgccgaa atattagctt
     6481 atgtgtattt ggcaatgaca ggtggtcaaa gcagtttatt tactgaggtt gaatccagtt
     6541 ttagtcaatc aaaaataaaa actcaagaga taaagtcatt aagtttaaaa aaaccggtaa
     6601 taatgaaggc taatgaagag gaattaatgc aacatcaaag tttcgttgaa tttatatcta
     6661 aaaaatccgg tatcaattat tgggaagagg tatagctctc atgattatat aaactataaa
     6721 taattattct gttacgctta ttattcggtt tcgcatctgc caatactaaa ctttatacaa
     6781 taacaaaatt cttcagacat cttatttcct ggtagctatc ctcaaaacaa gcaacccaag
     6841 tgtttggtta taggcagagg cgaagcctaa tgattatctc aatcaagcaa tttttttatt
     6901 atctatttgg gaattagatg tttttcagca aaatcttttc cgatacattc tcctaatgat
     6961 cttccccatg tgccagctgt ttcgctattg attttatctg gcatgctggc atacagttca
     7021 tcttggcatt ttttggtgct ttcaggaatt aaagtcacac attgttcata agtcatttta
     7081 atttcatcaa atcgtttttt gagatccgga tcttgaataa atcctttgca gataagatct
     7141 ggtaataagg gagtcataga gtttaaccaa gcatccttag tcatctcatt aggatttgct
     7201 gtgtcctcag cattggatat taagggaaat aatgaagtgc ttagcacgag cgtggtagca
     7261 ataagtaatt tccttgacat gatttattgt ccttattttt agagtctcat atatatagta
     7321 ggacaaaaaa tacaattttt cctttattta gtaatttata gttacttgaa actaaatgat
     7381 tgattcctct gtacttcttc ttgccaacac acctatgtgc ttgccgcaca tcttaggcaa
     7441 tcatggctct acgggttgtt tggtaagtta tttaataatt gattagcagt ggcataaaag
     7501 tgatgaaata tttgaattgc tcaaaaatta aactaaaata ataaagtgac ggaaaaaaaa
     7561 taggttaaat catggaaaag tccaattttc gcttaaattt tttgtctcaa attttgctct
     7621 tcactttagg ggtattttta ttctgtaatg ggtacgcagc agggaatacc tttattttta
     7681 accccaatac attaacctgg aaagctatca gtgctaatgg aaaggtgata cgtacagggc
     7741 gaggatcagg tggaaaacac tactgttctg atgttcgcag atcatgtaaa acaccgtctg
     7801 gcgtttttag agtgattagc aaaggtggcc cagcatgccg atccagtcgt tatccgctgg
     7861 gtaaaggtgg agcacctatg ccatattgta tgttttttag taaattatat gcaattcatg
     7921 gttctccgga tgtgccaaat tataacgcga gtcatggctg tgtcagagtg aaacctcatg
     7981 atgcaagatg gttaagccaa aattttatta aagttggaac gaaagtaatt attaaatcct
     8041 attaatagca ttaattaatg cgcca
//
LOCUS       DBNCWK010000133         8027 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.133, whole genome shotgun sequence.
ACCESSION   DBNCWK010000133 DBNCWK010000000
VERSION     DBNCWK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.133"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            111..2327
                     /locus_tag="AC06IO_002439"
     CDS             111..2327
                     /locus_tag="AC06IO_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9875611.1"
                     /translation="MQQQDQAQIPQNSRSFSRNTARCFDTFMDTVDFLGRIGWPVIDL
                     AFRIWIAQQLLVSAILLTNHWDTAVYLAKNEYPVPWLSPEWEALLGILAQFVGGISLL
                     FGLFTRCGAAVIVGFAVMTQVYYQPIDLNLLWIALMLGYVLRGPGPLSLDNLFEHGFS
                     RSPLPFAMLIVTFVDRTREFFSSLYLFSLRMWLMISLLLINQHIQLIPSDALPLLMPW
                     LPLNSASIVFNNVSLFMAIFLGLGLATRVVAIFGLIVVLLSFTPGVTGFYLYWMMVMA
                     ILFISGPGILSLDHLIFHALKKHYPQLSGKPAFSLDGLPRVVIIGAGFGGIACAKALR
                     HMPVKITLIDRHNYHLFQPFLYQIATGSLSPADIAISIRSIFLEQFNAEILLGNVTDI
                     NKEERLVIADNFTIPYDYLVIATGATHSYFGKDSWAPYAPGLKTINDGTSVRSRIIKS
                     FELAEIAESEEERKQFLNFVIVGAGPTGVELAGAIAELARFGIVKEFRHFDPASANII
                     LVQAAPRILPTFSEQISQKAQHYLESMGVKVLVNSMVEQIDSDGVIINKERIYSKSVF
                     WAAGVAASPASKWLQLEADPAGRVKVNDDLTVAGYSNIFVIGDTAASNAWNGKPVPGI
                     APAAKQGGAYVAKVISKRIYNNNSRYKPFKYIHYGSLATVGRKAAVAEFDRFKISGEL
                     AWWFWGGVHVFFLVGSRNRLSVILNWLWSYYTFRANNLLITDDSLNKKNKSGTSEV"
     gene            2369..3133
                     /locus_tag="AC06IO_002440"
     CDS             2369..3133
                     /locus_tag="AC06IO_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HIO9875612.1"
                     /translation="MDKYLSANSLEGVIDNEFNMPAPRWLNTYPAGPYRFINREFFII
                     AYETDPDLLQAILPPDMELLEPVVKFEFIRMPDSTGFGDYTESGQVVPVRYKGEEGGF
                     TISMFLDCHAPIAGGREIWGFPKKLAKPKLFVEEDTLIGILKYGSIDIAIATMGYKHR
                     PLDAEKVLESVKKPVFLLKNIPNVDGTPLVNQLTKTYLTDITVKGAWTGPGSLELHPH
                     ALAPISNLYIKKIVSVSHFITDLTLPYGEVVADYLA"
     gene            3171..3443
                     /locus_tag="AC06IO_002441"
     CDS             3171..3443
                     /locus_tag="AC06IO_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HIO9875613.1"
                     /translation="MDKRPIQNTAVIAAALGAALASIYSYTDWLSSDEITVKRERCYN
                     VARAGKNDCATSQHSCAAQSTADRDPEAFIMLPKGLCERIVGGRSG"
     gene            3436..4749
                     /locus_tag="AC06IO_002442"
     CDS             3436..4749
                     /locus_tag="AC06IO_002442"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIO9875614.1"
                     /translation="MDSYSKLPLRIRKEIEAQQYTGELLVAGVQLGVIVLLSIINIFS
                     SAGYSPGAPVQSSSLGLWLFAILILLRLWFVYTNQLNPVFLGFFIVAEMALLLFIIWT
                     YYLQFETTPTINLKNPHINYVYVLIALRALRFEPVWVILSGLTAAIGWGIITWQTLSS
                     SGMNVITWDYVTFASTRSVYLGAVFDVILSVLLVTAIIALVLTRAKHTLFQAVEQTSA
                     AKDLSRFFDTSVVEKITTSEKSLQAGYGELRQAAIMFIDMRGFTKASVKLSPSELIGL
                     LAEYQRLLVPIIQKNNGSIDKFMGDGILASFGAVTPSNTYAADALRTVDEIVASVDVW
                     SENRRKKEQIVVGVGIGLASGEVVVGVIGYENRLEYTVIGEAANLAAKLEKHNKEEKT
                     QALTTRDTFLEAVKQGYEDKITKKELNARNVGGVGEPLDLVVLAK"
     gene            complement(4769..5584)
                     /locus_tag="AC06IO_002443"
     CDS             complement(4769..5584)
                     /locus_tag="AC06IO_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HIO9875615.1"
                     /translation="MPRMKQIQEFFLNTVLNQQDTAIFKYFCPNEITNEFRFDIYRNT
                     ILQNLRHSLEMTFPAIWKLVGKGCADGLALHFIQDKNNLPTTNRLDDWGEKFPLFLKN
                     NSTVSHLLYLKDIAEIEWLKHLSYCSRDCIVFDPQKLQKHLNDRVEKLILLFNPSVFL
                     YSSSYYLKSIFDLIENPMETGSIDFQQIPSYAVISRQKNQTVIHWITHDLFHFLCHIK
                     AGASLGFAYENTLENNPDFDLLSALQFLLRNELLEDSFINPNYHLTHTNNCPE"
     gene            complement(5577..6404)
                     /locus_tag="AC06IO_002444"
     CDS             complement(5577..6404)
                     /locus_tag="AC06IO_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HIO9875616.1"
                     /translation="MHKAINGAMTGIGLRAPHYAQILEEKPDIDWLEVHTENFLAEGG
                     PLLELLDSISQHYPLSFHGVGLSLGSAEGVSTAHLNRIKTLIDRFQPALLSDHLSWST
                     IGNTYLPDLLPIPYNEESLTIISNNIDRVQNYLKRTLLIENPSSYLEYQTSSYSEPDF
                     LAEIIKRTEARLLLDINNIYVSCSNNKRDPYQYIQRIPHEAVQEIHLAGHSIVYQDNT
                     NFLRIDTHNNYVCPEVWDLYRYAIHYTGLVPTLLEWDIEIPSLDTLLNEARKSACYA"
     gene            6689..6955
                     /locus_tag="AC06IO_002445"
     CDS             6689..6955
                     /locus_tag="AC06IO_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875617.1"
                     /translation="MSQNWPTRDKDLQTARVIMEEYANERDSDSLGLFEIVVDQNEKR
                     MNFRLSGWVVILAKHFTSLYGVSQGDYVTRQVISRCITQGQTLH"
     gene            complement(7210..7962)
                     /locus_tag="AC06IO_002446"
     CDS             complement(7210..7962)
                     /locus_tag="AC06IO_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIO9875618.1"
                     /translation="MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTN
                     NELFGYDIATMTELCKIMKRQCNFRVMRFRQLIPALINKQIDAAISAMSITSERSNLV
                     NFSMPYLLSYSRFLTNEVHNTHQPFNLMLLKGKRIGVEQNTVFSQEIRRMGIIDPVIV
                     EFDRTEEMLEALSANKIDFVLMDNPAALYWSANSSGRLLVIGSPMLVGYGLGIAVNRE
                     NSVLLKSLNDALVEYQRSDKYKENYNKYMMNF"
BASE COUNT         2366 a         1412 c         1672 g         2577 t
ORIGIN      
        1 ttatcagaat gatagtccac aacataattt gaaatttgca gctataatta aatccaaagt
       61 tattaggaat acaaagatat tttaaaaact ttcttgaaag ggattttgat atgcagcaac
      121 aagatcaggc acagatacct caaaacagtc gttcgttttc aagaaacacg gcgaggtgtt
      181 ttgatacttt tatggacacg gttgactttc ttggccggat tggatggcct gttattgatt
      241 tggcatttcg aatatggata gcccagcaat tattagtttc cgcaatactt ctaaccaacc
      301 actgggatac tgctgtctat ttagctaaaa atgaataccc ggttccatgg ctatcacctg
      361 aatgggaagc tttgttaggc atattggcgc agtttgtagg ggggatttcc ctgctttttg
      421 gtttgtttac ccgatgcgga gcggcggtta ttgttggatt cgctgtcatg actcaagtct
      481 attaccagcc tatcgatctt aatttgcttt ggattgcatt aatgttgggt tatgtattgc
      541 gaggcccggg tccactttct cttgataatt tgtttgagca tgggtttagt cgcagccctt
      601 tgccgtttgc catgttgatt gtgacatttg tagatagaac aagagaattt ttttcaagtt
      661 tgtatttatt ttctttacgt atgtggttaa tgatttcact tttactcatc aatcaacaca
      721 tacagctcat tccttctgat gcgctgccgc tattgatgcc atggttgcca ttaaattcag
      781 cctctatcgt ttttaacaat gtttcactat tcatggctat ttttttgggg cttggcttag
      841 cgactcgagt tgttgcaatt tttggattaa tagttgttct tttgagtttt acgcccggag
      901 taaccggttt ttatttatac tggatgatgg tgatggctat tttatttatt tcgggcccag
      961 ggatcttgtc attggatcat ttgatctttc atgctttgaa gaaacattat ccccagcttt
     1021 ctggaaaacc ggcatttagc ctggatggtt tgccacgagt agttattata ggtgcaggtt
     1081 ttggggggat tgcttgtgct aaggcactac gtcatatgcc agtcaagatt actttgattg
     1141 atagacacaa ttatcatctt tttcaacctt ttttatacca gattgctact ggaagtttat
     1201 caccagccga tattgctatt tcgattcgct ctatattttt agaacaattt aatgcggaaa
     1261 tattattggg taatgtaaca gatattaata aagaggaacg ccttgtcata gctgataatt
     1321 tcacaattcc atatgattat ctggtaatcg ctacaggagc aacgcacagt tattttggaa
     1381 aggacagttg ggccccctat gctcccgggt taaaaacaat taacgatgga acttctgttc
     1441 gaagccgtat aataaagtcg tttgaactgg ccgaaatcgc tgaaagtgag gaagaacgca
     1501 agcaattttt aaattttgtc attgtaggcg ccgggcctac tggagtcgaa cttgcaggag
     1561 ccatagcaga actggctcga tttgggatag taaaggagtt tcgtcatttt gacccagcat
     1621 cagcaaatat catactggtt caggcagcac cgcggatttt acccaccttt tcagaacaaa
     1681 tatcgcaaaa agcacaacac tatttggaaa gcatgggggt taaagtttta gtaaatagta
     1741 tggttgagca aattgatagc gatggtgtga ttattaataa ggaaagaatt tattcaaaat
     1801 cagttttttg ggcagcaggt gttgctgctt cccctgcatc aaaatggctt cagctcgaag
     1861 ctgatccagc gggtcgcgtg aaagtgaatg atgatctcac cgtagccggc tattctaata
     1921 tttttgttat tggagatacg gctgcttcga atgcctggaa tggcaagccg gttcctggta
     1981 ttgctcctgc tgcaaaacag ggcggtgctt atgtagcaaa agtcatatcc aaaagaattt
     2041 ataataacaa ttcaagatac aaaccattta aatatattca ttatggtagc ttggctacag
     2101 tgggtcgtaa agcggctgtt gcagaatttg atcgtttcaa aattagtggg gaactggcat
     2161 ggtggttttg ggggggagtc catgtctttt tcctggttgg ctctcgaaat cgcttaagtg
     2221 tcattttaaa ttggctttgg tcttattaca cttttcgcgc caataatttg ctgattaccg
     2281 atgattcatt gaataaaaaa aataaatcag ggacgagcga ggtttagggc tcattgaaga
     2341 taataaggaa atacaaaggg agtgattaat ggacaagtat ctttcagcaa attctctaga
     2401 aggggttatc gataatgaat ttaacatgcc agctccgcgt tggttaaata cttacccggc
     2461 tggcccatat cgatttatta atcgtgaatt ttttattatt gcttatgaaa ccgatccgga
     2521 tcttttgcaa gctattttac ctcctgatat ggaattattg gagccggtag tcaaatttga
     2581 atttatacgt atgcctgatt caacaggatt tggtgattac actgagtcag ggcaagtggt
     2641 ccctgtgaga tataaaggag aagagggcgg atttaccatt tcaatgtttc ttgattgcca
     2701 tgcccctatt gctggtggcc gagaaatatg gggttttcca aaaaagctgg ccaaacccaa
     2761 attgtttgtt gaagaagaca cgctcattgg cattcttaag tatgggagta ttgatattgc
     2821 catcgcaact atgggatata aacatcgtcc gctggatgcg gaaaaggtat tggaatccgt
     2881 taaaaagcct gtatttttac ttaaaaacat tcctaatgta gatggaactc ctctagtgaa
     2941 tcagttgacc aaaacttatt tgactgatat tacagtgaaa ggagcatgga ccggaccagg
     3001 tagcttggag cttcatcctc atgcactggc tcctatctct aatctttata ttaaaaaaat
     3061 tgtatccgtt tcacatttta ttactgattt gaccttaccg tatggagagg ttgttgccga
     3121 ttatttggcc taaaataaaa agcgactcct aaaaatggca ggaaaatcat atggataaac
     3181 gtccaataca aaacacggct gtcattgcag ctgctttggg ggctgctctt gcttctatat
     3241 acagctatac agattggctt tcatcagatg aaatcacagt aaaaagagag cgttgctata
     3301 atgtggcaag agcgggcaaa aatgattgcg ctacttccca gcattcttgt gctgcgcaat
     3361 caacggccga tcgtgaccct gaagcattta tcatgttacc taaaggatta tgcgaacgta
     3421 tagtgggagg aaggagtgga tagttactct aagttaccgc ttcgcatcag gaaagagata
     3481 gaagcccaac aatacactgg tgaactattg gttgccggtg tccagttggg tgtcattgta
     3541 ttattaagca ttatcaatat attttcttca gctggctatt cgcctggcgc gccggtgcaa
     3601 tcttcatccc tgggcttgtg gctatttgct attttgatat tactaaggct gtggtttgtc
     3661 tatactaatc aattaaatcc agtattcttg ggatttttta tagttgccga aatggctttg
     3721 ttattattta ttatttggac ttattatttg cagttcgaaa ctacacctac cattaatctt
     3781 aaaaatccgc atattaacta tgtttatgtt ttaattgctt tgcgcgcgct tcgatttgaa
     3841 ccagtatggg taattctctc tggcctcact gcagctatag gctggggaat tattacctgg
     3901 caaactttat caagctcagg catgaatgtc attacatggg attatgtgac attcgcgtca
     3961 actcggagtg tctatctggg agctgtattt gatgtcatat tgtccgttct cctggttaca
     4021 gccatcattg ccttggtatt gacgcgtgcg aaacacactc tttttcaagc agtagaacaa
     4081 actagcgctg caaaagattt atcacgtttt tttgatacaa gtgtggtcga aaagataaca
     4141 acatctgaaa agtcattgca agctggatat ggcgagcttc gtcaggccgc aattatgttc
     4201 attgatatgc gaggatttac caaagcatca gtgaaactgt ccccgagtga actaatcgga
     4261 ctgctagcag aatatcaacg cttactggtt ccgattattc aaaagaataa tgggagcatt
     4321 gataaattca tgggggatgg tattttggca agtttcggtg ctgtgacccc ttcaaatact
     4381 tatgcggcag atgcattaag gactgtggat gaaattgtcg catcggtaga cgtatggagt
     4441 gaaaatcgcc gtaaaaaaga acagatagtg gtcggtgtag gaattggtct tgcttcaggt
     4501 gaagtagtag ttggtgtcat tggttatgaa aatcgtttgg aatacactgt aattggtgaa
     4561 gccgccaatt tagctgcaaa gcttgaaaaa cataataagg aagaaaaaac ccaggctttg
     4621 actacccggg atactttttt agaagcggta aaacaaggtt atgaggacaa aataactaaa
     4681 aaggaattga atgcaagaaa tgtaggtggt gtgggagaac cattggattt agtggttttg
     4741 gcaaaataac ctatgctgct cttcacagtc attcaggaca gttgttagtg tgagtcaaat
     4801 gataattagg attaataaaa gaatcttcca ataattcatt tcttagtaaa aattgtaatg
     4861 cactgagcaa atcaaaatcg ggattatttt ccagagtatt ttcgtatgcg aacccaagtg
     4921 aggcccccgc tttgatgtgg cataaaaagt ggaacaaatc gtgagtaatc caatgaatca
     4981 ctgtttgatt tttttgacga gatatcactg cgtaacttgg tatttgttga aaatcaatag
     5041 aacctgtttc catgggattt tctatcagat caaaaatact ttttaagtaa taagaggatg
     5101 agtataaaaa cacggaagga ttaaatagca aaattaattt ttctactcga tcatttaggt
     5161 gtttttgcag tttctgggga tcaaaaacaa tacaatcccg tgagcaatag cttaaatgct
     5221 ttaaccattc tatttctgcg atatctttca aataaagaag atgactaacg gttgaattat
     5281 tttttaaaaa cagaggaaat ttttcccccc aatcatccaa acgattggtt gttggcaaat
     5341 tatttttatc ctggataaag tgtaatgcca aaccatccgc acatcctttg ccaaccaatt
     5401 tccaaatggc tggaaaagtc atttccaggg aatgccgcag attctgcaaa atggtattac
     5461 gataaatgtc gaaacggaat tcattagtaa tctcattagg gcaaaaatac ttaaaaatgg
     5521 cagtatcttg ttgatttaat actgtgttca gaaaaaattc ctgtatttgt ttcattctag
     5581 gcataacagg cggacttcct agcttcattt aataaagtat ccaacgaggg aatttctata
     5641 tcccattcta acaaagtggg aacaaggcct gtataatgta ttgcatatcg atacaaatcc
     5701 catacctcgg gacacacata attattatgt gtgtctatcc gcaagaaatt cgtattgtcc
     5761 tgatagacaa tggaatggcc cgccaaatga atttcttgta ctgcttcatg cgggattctt
     5821 tggatatatt ggtaaggatc tcgtttgtta ttagaacatg aaacatagat attatttata
     5881 tccaataaga gcctggcttc agtacgcttg attatttctg ctaaaaaatc gggctctgaa
     5941 tacgatgaag tctgatattc caaataagaa gaggggtttt ctattaataa ggttctcttt
     6001 aaataatttt gtactctatc aatattattg ctaataatag tcaaactttc ctcattatag
     6061 ggaataggta ataaatcggg taaataggtg tttccgatgg tactccagga caaatgatca
     6121 gaaagcaaag ctggttgaaa gcggtcaatg agagttttaa tgcgatttaa atgcgctgta
     6181 gagactccct ctgcagaccc taaagataat ccgactccat gaaagctcag tggataatgt
     6241 tgacttatag aatctagaag ctctagtaaa ggcccaccct cggccaagaa attttctgta
     6301 tgtacttcca gccaatcaat atcaggcttt tcctctaata tttgggcata atgtggtgct
     6361 cttaggccaa tacctgtcat agcaccattt atggctttat gcatgatatt tttttgtccg
     6421 acttgaagtt gtattgagca attaaaaact atgccaaaga tttaatgata tattaatgta
     6481 ctcttgagaa gcttctaacg caatggtctt ggagaaagct attttaaatc ataagtgttt
     6541 ttaattcagt gtaaaaatta accgcaatat tgagtattta atcactttat gagataatat
     6601 ttatttattt cacaacttgc aattttgaaa attgcctcca tgttgtagaa gtaacaacag
     6661 tcttgctcat gtttttaggg agtttcttat gagtcaaaat tggcctacca gagataaaga
     6721 tttgcaaacg gcgcgagtca taatggaaga gtatgctaat gaacgtgata gtgattcatt
     6781 aggtcttttc gagattgtcg ttgatcaaaa tgaaaaacgc atgaatttcc gtttatctgg
     6841 ttgggtagtt attttggcaa aacattttac ttctttatat ggagttagtc agggtgatta
     6901 cgttaccagg caggtgatta gtcgctgcat tactcaaggt caaactctgc attaaaagct
     6961 agagatgagc cctcaattgg aatggctgta ttgggttcat attgaaagaa aataatcctt
     7021 cgaaatgctc acatagtcat ctatgctgtg tttttttatg attattttca tccaagctgg
     7081 actcagatcc cgcccttaga gaatagttaa atatcaggac gttgacttat tacgtcttaa
     7141 aacgattatt cagctattga tgaatgatta agagcaattt ttgtagcaat tgccccaacc
     7201 acatcactct taaaaattca tcatgtattt attgtagttt tccttgtatt tgtcagaacg
     7261 ctggtattct accaaggcat catttagaga tttgagtaat acggagtttt cccgattgac
     7321 tgcaattccc aaaccataac caaccaacat gggactgcct attaccagaa gtctccccga
     7381 cgaattagcg gaccagtata aagccgcagg attatccatc agaacaaagt caattttatt
     7441 tgcgctcaac gcctcaagca tttcttcggt tctatcaaac tccactatga cagggtctat
     7501 tatccccatc cttctgattt cttggctaaa gacagtattt tgttccactc caattcgttt
     7561 accttttaag agcattaaat taaacggttg atgagtattg tgtacctcat ttgtaagaaa
     7621 cctggagtaa ctcaataaat agggcatgct gaaattaacc aggtttgaac gttctgaagt
     7681 aatagacatg gcgctgatcg ccgcatcaat ttgtttgtta atcagagcag gaattaattg
     7741 tctgaatctc atgactcgaa aattacattg gcgtttcatt atcttacaga gttctgtcat
     7801 ggtggcgata tcatagccaa aaagctcatt attggttcct tgcatgataa agggaggata
     7861 aaagcctcct acacctacta ttaaaggtat aggctcgttt tgagcgtgga gaggtgaaac
     7921 tagaaaaaag aaagaaagca ataatccctg aataaatttc attggtatct tcactaaaat
     7981 attattagag tcttttataa gagtataacg tccaatagga gttttaa
//
LOCUS       DBNCWK010000134         7860 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.134, whole genome shotgun sequence.
ACCESSION   DBNCWK010000134 DBNCWK010000000
VERSION     DBNCWK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.134"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(8..1390)
                     /locus_tag="AC06IO_002447"
     CDS             complement(8..1390)
                     /locus_tag="AC06IO_002447"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIO9875619.1"
                     /translation="MKLTPSQMRSAIPGVIWPPAPHPMAAQLYYQLDNFNHTEKLPPS
                     EIIKNQFKQLVALISFSRQCVPYYQKKFAYLPSFEKWQDLHKAWQELPVLKRETLQIT
                     KDNIFALQVPPGHEPSELLSTSGSTGQPVTVKGNMATQFFWNAISLRNHLWHNDEFHK
                     SFASIRYTENKEALPPLGAKFLNWSPATYSIVETGPCYQLTLCTPEQEAQWLQRINPD
                     YLNCNPSTLREITLYFAKHGNKPENLQKVHTHSEIVEPELRTLVKEVLGIPLIDNYSS
                     KECGYIALECPISGYYHVQSENVLVEILDESDNPCKPDQPGRVVITTLHNFSSPLIRY
                     DIGDYAIPGESCPCGRNLPVLKYILGRKRNMIQMPDGRKLWPAFASNGLRLMDLFSGS
                     QFQLVQTTPTAIQVNLKHPDSLTSLEEQKIREKLSIIFNYPFHFIFNYVDSIPRSAGG
                     KFEDFISLIS"
     gene            complement(1435..2412)
                     /locus_tag="AC06IO_002448"
     CDS             complement(1435..2412)
                     /locus_tag="AC06IO_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HIO9875620.1"
                     /translation="MTALRKTTLSLFIIGFCNTQSSIAGTMGPVNTGCTGNRLFAGAD
                     GLYLQPRNDDLDYATFLVDNYTKTLNTYLDYDWGFRLYGGIKFANNNDITIAWQRLHT
                     RDNDLLLGDGNIAIEPRWLNLAPWTSVQSENIFDYDEVSAVFAHTHDFNNAWLIRYGA
                     GAEYAEIDSRLTVNGIAQTIQGHTSFSNFQGFGPRIEADIFYNFYRDFNIFASANSAL
                     LIGSRDVSLLAHHPFGVTRDFSDRNTVVPKLGLRVGLDYQRPFGLIGDGAVSTIDFQV
                     GWQAETYIDAIERASTGLVGGEGVVILPQRSNYSNQGLFFGVKLTSGCL"
     gene            2818..4724
                     /gene="pieE"
                     /locus_tag="AC06IO_002449"
                     /pseudo
     CDS             2818..4724
                     /gene="pieE"
                     /locus_tag="AC06IO_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieE"
     gene            complement(5314..6063)
                     /locus_tag="AC06IO_002450"
     CDS             complement(5314..6063)
                     /locus_tag="AC06IO_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875621.1"
                     /translation="MNKVKAKIIAFTTVMLFLTSINIHAKGETPMKQYHQINEATMIT
                     MKSKLPNSSEFEKFLESGGQLVKQTEPDTKVWFALKGNDNSLMIFDAFYDSKGREAHF
                     AGQVAKALKNNSEQLVLEGWENGVLKNIVNSKVLASKLPARSVPVTLANYIEFTANDG
                     KSEQLAALLSNAAEIVDKTEPQTAYWFALQLNDNTFAIFDAFSDESGQKAHFSGKVAL
                     SLKNHSELLIKNGWEKGVLPHIKSLKVITNI"
     gene            complement(6122..6682)
                     /gene="psrA"
                     /locus_tag="AC06IO_002451"
     CDS             complement(6122..6682)
                     /gene="psrA"
                     /locus_tag="AC06IO_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor PsrA"
                     /protein_id="HIO9875622.1"
                     /translation="MNISNTKERILAVAEALIQKDGYNAFSFKDIATAIHIKTASIHY
                     HFPSKEDLGVAVISWHADKIAAVLSDISNNSSLSAKEKIQKFFDVILTITYNAENKMC
                     LGGMFASDFQSLPVSIQNQAKKFFELIIEWLKGVLEASAYDNESALSVAKQIISLIEG
                     GLLLARLYGDETFLEGVRHFIDQTIK"
     gene            6944..7171
                     /locus_tag="AC06IO_002452"
     CDS             6944..7171
                     /locus_tag="AC06IO_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875623.1"
                     /translation="MFFKRSNPHVTPQDLQKVIQNLNAQRELTERQLKEGSISQKTGQ
                     EEMQRLSSLIGAYQNNLMAALDDQQHTNCPK"
     gene            7505..>7860
                     /locus_tag="AC06IO_002453"
     CDS             7505..>7860
                     /locus_tag="AC06IO_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HIO9875624.1"
                     /translation="MIKDILNTIASLIEENTEVFASERGKQLLQKVKADLASFKPINF
                     YALDAEKQITDALKLIESYCIEMNRILDELNHQSSVSLANEKLINKIENILTNLKNKQ
                     NKITQLREKIPEGMLDV"
BASE COUNT         2529 a         1412 c         1499 g         2420 t
ORIGIN      
        1 tatataatta tgaaatcaat gaaataaaat cttcgaattt tccaccagca ctgcgtggaa
       61 tagagtcgac ataattgaag ataaaatgaa aggggtaatt aaagattata ctgagttttt
      121 cccgaatttt ttgttcttcc agggatgtca atgaatcagg gtgttttaaa ttcacttgaa
      181 ttgcggttgg agtagtttgg acaagttgaa attgagagcc agagaagagg tccatcaggc
      241 gtaaaccatt actggcaaat gctggccata gttttctacc atcaggcatt tgtatcatat
      301 tgcgtttcct gcctaaaatg tattttagca cgggtaagtt tcgtccacag ggacaacttt
      361 cgccgggtat tgcataatca cctatatcat aacgaatcag gggagaggaa aaattatgaa
      421 gggtggtaat gacaacacgc ccaggctgat ctggtttgca tggattatcg ctttcatcca
      481 ggatctcaac aagtacgttt tctgactgca cgtgatagta cccgcttatc gggcattcaa
      541 gcgcaatata gccgcattct tttgaagagt aattgtcaat cagaggaata cctagcactt
      601 ctttaaccag ggttcgaagc tctggttcta cgatttcact gtgagtatgc accttttgca
      661 ggttttcagg tttatttccg tgtttggcaa aatacagtgt gatttctctt aaggtagaag
      721 ggttgcaatt caaataatcc ggatttatgc gttgaagcca ttgtgcttct tgttctggag
      781 tacagagagt aagttgataa caaggtcctg tttcgacaat cgagtaggtc gcaggactcc
      841 agtttaaaaa ttttgctcct aatggaggaa gagcctcctt attttccgtg tagcggatac
      901 tggcaaaaga tttgtgaaat tcatcattat gccaaagatg atttcgcaga gagatggcat
      961 tccagaaaaa ctgggttgcc atattacctt ttacggtgac tggttggcca gtggaaccgc
     1021 tggtagataa taattctgat ggttcatggc ccggcggcac ttgcaaggca aaaatattgt
     1081 cttttgtaat ttgaagcgtt tctctcttca acacagggag ctcttgccag gctttatgca
     1141 agtcttgcca cttttcaaat gagggtaaat aggcaaattt tttctgataa tacggaacac
     1201 attgcctgga aaaacttatt aatgccacca gttgtttaaa ttgattcttt ataatttctg
     1261 aaggtgggag tttttctgta tgattaaaat tatccagctg ataatacaat tgagctgcca
     1321 ttggatgtgg cgcaggcggc cagataactc ccggaatcgc gctgcgcatt tgtgagggtg
     1381 ttaacttcat gttactcaac cgtaagctca attacattgg aataatgctt agaattacaa
     1441 acaaccactg gtgagtttca ctccgaagaa taatccttga ttgctgtaat tggatctttg
     1501 aggtagtata accactccct caccaccgac caggcctgtc gaggcacgtt caattgcgtc
     1561 gatataggtt tcagcttgcc aacccacttg aaaatctata gttgaaacag cgccatctcc
     1621 tattaaacca aatggtcgtt gataatccaa tccaacgcga agccccagtt taggcactac
     1681 ggtgtttcgg tcagaaaaat ctcgagtgac gccaaaagga tggtgcgcaa gtaaagaaac
     1741 atctcttgag ccaatcagta aggctgaatt tgcgcttgca aaaatattaa aatctctata
     1801 aaaattataa aaaatatcag cttcaatacg tggaccaaac ccttggaaat tactgaagct
     1861 ggtgtgtcct tgtatcgtct gtgctatacc atttacggtt agtcttgaat caatttctgc
     1921 atattctgct ccagcaccat aacggataag ccaggcatta ttgaaatcat gggtatgagc
     1981 aaaaacggcc gagacctcat cgtaatcaaa aatgttctcg ctttgtacag atgtccatgg
     2041 tgcaagatta agccaacgag gttctatagc aatattacca tcacccaaca aaaggtcatt
     2101 atctcttgta tgtaatcttt gccaagctat agtgatatcg ttattatttg cgaattttat
     2161 cccgccgtat aatctaaaac cccagtcata atccaaatag gtattgagtg tttttgtata
     2221 gttatcaacg agaaaggttg catagtcgag atcatcatta cggggttgca ggtagagtcc
     2281 gtctgctcct gcaaacaggc gatttccggt gcagcctgta ttaactggtc ccatagtgcc
     2341 tgcaatagac gattgtgtat tacaaaatcc aattataaag agacttagag ttgttttcct
     2401 taaagcagtc attaatatca ttctcctttg atagatgctc ttggttaagt cggctgtata
     2461 atataatgga taaaaatgga tattaaaagt aaaaaagcgc tgccttggtc gagaaagact
     2521 ttaattagct catcataaat taacctgttg atttatcatc taaaaatagt gagtggatac
     2581 taggctttga gatttggaat gcacagctga aaagaggatt gggttttact tttcctccct
     2641 tgctgaaatc acatttcaag tttggcttat tctcaaagtc tgtaattttg ttcagtctta
     2701 acaattcctt aacaatccct taacaattcc ttaacatttg tctgccataa tgccctcgat
     2761 ttaaacacga aaacaaagcg cagggggtta tcacactgtt ttgaatagaa ctaggaaatg
     2821 gagtctgtat tttcaggggg agtaaatttc ttctgcatca tgtatcgatt catatgaata
     2881 tttcaacata cgaattcgtt aataatacaa ttactgagga gtcacgagtc atggcgttat
     2941 cagaaatcat tatcaatgat atagttcaat ctcaaactaa tgcagtcatt agcgaatcaa
     3001 attaccatat tcttcgccaa tacctactgg aaagaaaacc tgtatcaaga gtactcaatc
     3061 caatcaattc tctgctttct gagcattgca atcaagataa gcaagcaata ataaatcact
     3121 taactcaact ggcttgtgag tctcaaagaa atcatgactt acaagaagca agaagtgatg
     3181 aacaagaggg aatcaatgaa aattctttga tgattaatta caggagcgag ttgcttactt
     3241 tggaaaatac attgaaggaa ctggatacaa aatgctatca acaacaacgt caatataatc
     3301 aggtgagtag gcaatttaat gaacttaaag tcaatatgac ccacattaat caaagcattg
     3361 atagagttcg ttatgaacgt caattgcttg accagcagct aaaccaccct tactctgatg
     3421 gtaatgttta tactaatccc cctactcaaa cacgtattta tcctgatctt tcaccacctc
     3481 aggatcggca tactcgtgat agactgttgc aagaagaaaa cagattaatt gaggagcgtc
     3541 aaaagctagt acatctcatt aattcaaaag aaaccgaaaa aaacaaagaa gagcaaactt
     3601 tgaataggct tcataaggag aaaaaggaag ctgaagagcg ttatagtgaa gtaaaacatc
     3661 aaatcgatgt tgttttgcca aatagtgaac atcagcgtca aatcagaaat caagaacgtc
     3721 tcgctcgaga acaggccaga gcaatctatg atccacattt attgcaactt tctcataaga
     3781 atcttgaagc actgaaatct cagattgaaa ctcagattcg cgagctggat gagcgacgaa
     3841 atcagttaat gggcgaagcg agagacataa gttataaaat ctatttaact caattggagc
     3901 aggttcttca agaatcagaa aatgggcctc aaattacgtt caatgaaaat aatgccttaa
     3961 agatgatttt agccataatg aaaaacatgg aggaaatggc ggaaaaagag aaagatatag
     4021 ctagctcaat cgataaagag cgacataatc tccattcatt gcaaaaaagc cttgtaggat
     4081 acaccaggca actggaacgg tatctgacgt cagatcccca tttggttaag caaaacaagg
     4141 ctcttaccga agaaaataca cagttacagc aatatagtga atcagcagac aattggagaa
     4201 cgaaagcatt ctatgcgtct cttttcagtg ttgggggtag tttaataagt atcggaatat
     4261 tgaatacatt catcattagt cctgtatttt ttgcgattcc cggtgctctt gctgcgcttg
     4321 gtgtggtttc ccttgttatt gcggtagtct cttattgcca gaaatacttt agtgatatgc
     4381 aaatggaaca aaacaatcag aagattgaga aaaatgaatt aatactaatg aagcaatgga
     4441 aaaaggctaa tgaattaagt ttatctataa ttccaagcct gaacgctaaa atagaactat
     4501 cagagaaaga gctgtatgcg cttgagcaaa aattacagga tcagcagcac gcaatgagtc
     4561 tgctgttgaa taaagcgcaa aatgttacca gcaattatgg aggaagcagt aatttctttg
     4621 gtaacacgac tggtaatgtg ttctatttac cttctgcccc tctgcatgag gactctccac
     4681 tgtatccaga gattgaaggt gaaagagtaa attacggcta ctaataagct aaaaatgact
     4741 ggtctttaat ttggtttcaa tttgaagacc agtatctctg attattcaaa tcctgaattg
     4801 ctctttacaa tctgctttgt aaaaatattt tgttcatact ggatttaaaa agaagtattt
     4861 tttagttaat gctgtgtggt ttatgaggct tggggctgat ttaaaggcag tatttttatt
     4921 gcagcctcat taatgtgcta aaattgtaca catgggtaac ctgatatcat tgcttgaatg
     4981 ctaaattaca ttcccttatt gaaaaggatt tccatatgaa aacagtccta atattaatta
     5041 tttttttatg ttcttttcaa atacaggcgg catgcaaatg caactgtgac ccgacagaca
     5101 ataagatgtg tgcgagtcag aatgatctcg agcatccttg cccttcagtt tgtgcttcgc
     5161 cagcacctgg tatggcgcct tcgtttacag catgccctgt atcaagggtt actgatccag
     5221 tcacaggagt taccagatgg gtcaatatgt gtaactaata tcaaataatc cattattttc
     5281 aggaaaaatt ttatctttaa tttacctgac tgattagatg ttggttatca ctttcaggct
     5341 tttaatgtgg ggtaagacgc ctttttccca tccattttta atcaagagtt cagagtgatt
     5401 ttttaaagat agtgcgactt ttcctgaaaa atgagctttc tgaccagact catcagagaa
     5461 cgcatcaaaa attgcaaagg tattgtcatt cagctgtaaa gcaaaccagt aggctgtttg
     5521 aggctcagtt ttatcaacga tctcggcggc attacttaat aacgctgcaa gttgctctga
     5581 tttaccatcg ttggcagtga attcaatgta attggcaaga gttactggaa ctgatcttgc
     5641 aggtaattta gaagcgagca ctttactgtt tacaatattt tttaatacgc cattttccca
     5701 accttcaaga accaattgtt cagaattgtt ttttagtgcc ttggcaactt gaccggcaaa
     5761 atgggcttct cttcctttag aatcataaaa tgcatcaaag atcatcaagg aattatcatt
     5821 acccttaagg gcaaaccata cttttgtatc aggttcagtt tgctttacca gctgaccgcc
     5881 agattccaaa aatttttcaa attcagagga atttggtaat ttgcttttca ttgttatcat
     5941 ggttgcttca tttatctggt gatattgttt cataggtgtt tctccttttg catgtatatt
     6001 tatcgatgtc aaaaacaaca ttactgttgt aaaggctata atttttgctt tgactttatt
     6061 catataacgt ttccccataa aattctatct actaatagat agtttcgaat gttaaaaaaa
     6121 atcactttat tgtttgatca ataaagtgcc gaactccttc cagaaaggtt tcatctccat
     6181 ataatcttgc taataataac ccgccttcaa tcaacgaaat aatttgtttt gcgacagata
     6241 atgcggattc attatcataa gcacttgctt caagaactcc tttaagccat tcaatgataa
     6301 gctcaaaaaa tttttttgcc tgattttgaa ttgaaacggg taatgattga aaatcagaag
     6361 cgaacatacc tccgaggcac attttgtttt cagcgttgta ggtgattgtt aaaatgacat
     6421 caaagaattt ttgaattttt tccttggctg ataacgacga attattgctt atgtcagata
     6481 acacagcagc aattttatcg gcatgccagg atataactgc cacaccaaga tcttcttttg
     6541 aaggaaaatg ataatggatg ctggcagttt taatatggat agctgttgca atatctttga
     6601 aactaaaggc gttatatccg tctttttgga tcaatgcttc cgcaactgct aatatccgtt
     6661 ctttcgtatt acttatattc ataggcatga attttaccta ctcatagata gattgtcaag
     6721 ctttttatag ttttttatat caaattaaaa taacactcgt ttatataaat agaagtggat
     6781 ggtggactgc tgattaattc attccgatct ttattgggtt gatttatcgg atcaatatct
     6841 tggtacgtcg ccatgtcaat tggccttaaa gactttatgt ctttattgat ctcgaaaaaa
     6901 acgggaggta atatatgtgc atcagttcac aatagaggtc attatgtttt ttaaaagatc
     6961 aaaccctcac gtaacaccac aagatttaca aaaagtgatt caaaatttaa atgcgcaacg
     7021 tgaattaaca gagcgtcaat tgaaagaggg ttctatatca caaaagacag ggcaagaaga
     7081 gatgcagcgt ttatcaagcc tgattggagc atatcaaaat aatctgatgg cagcattaga
     7141 cgatcaacaa cacactaatt gtcctaagta gctaatcttt ttgaggtata agagggcagt
     7201 cattttgatt aatggcataa tccactcata atctgaatgg tcatttacgt gttgatagga
     7261 ttttgagcac tctgtgcaca ttctatgtac caagaaaaca gggatgttat tgccctatca
     7321 atgtattcga agtcttcccc agaccttaat tttaagtgat gttcatgagt ctatttaaga
     7381 gattggaaat aaaatagatt attgaaattt atgaataatt aacccagaaa gtacttgctt
     7441 tattcaatct gtacaggtat ttctattcaa tcaaagccca ttctgtatag caagggagta
     7501 ttaaatgata aaggacattc tcaataccat agccagtctt attgaggaaa acacagaagt
     7561 atttgcaagt gaacgtggaa agcaactttt acagaaagtt aaagccgatc tcgcatcgtt
     7621 taaaccgatt aatttttatg cattggatgc agaaaaacaa ataacagacg cgcttaaact
     7681 aatagagagc tattgcattg aaatgaacag aatattagat gaattaaatc atcaatcttc
     7741 agtttcttta gccaatgaaa aattaattaa taaaatagaa aacattttaa ctaacttaaa
     7801 aaacaaacaa aacaaaatca cccagttacg agaaaaaatt ccagagggca tgcttgatgt
//
LOCUS       DBNCWK010000135         7814 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.135, whole genome shotgun sequence.
ACCESSION   DBNCWK010000135 DBNCWK010000000
VERSION     DBNCWK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.135"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            169..1014
                     /locus_tag="AC06IO_002454"
     CDS             169..1014
                     /locus_tag="AC06IO_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIO9875625.1"
                     /translation="MKYQQKLENMIDFIGKHLDEELSLESLSEIFCISKFHFHRLFTA
                     FTGLSLQQYIKWLRLKRAAHQLVVEKDQSVINIAINAGFESHEAFSRAFKKSCGFSPS
                     QFRQGFGRSYWEQPPYCLPRQGRIDMKVDIKSIDKIRLAVIEHKGDPKLLGESINKLV
                     SWAKSQSINLKPRPGEAFALAYDDPKTTPPSEFRIDLGIKVPENLKLDGVIEKFLPSG
                     RYAVTVHKGSRNNIGDVVYYLYRDWLPNTSEQLGDLPCIFCYYNFDHEVAETELLTEC
                     WLLLK"
     gene            complement(1242..2090)
                     /locus_tag="AC06IO_002455"
     CDS             complement(1242..2090)
                     /locus_tag="AC06IO_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIO9875626.1"
                     /translation="MSKEINIIWVGPQRLPYKYLQHLKKWHSLNPDYQINFITDEHSS
                     SIQLPGINTIHWKQCFDLTDVRQEFLLREAERALREKAYATLSNIVRKVKIFNDGGYY
                     FDTDVVPIEKLPAITENTPFLSQPKASYGAEFAKHRFVVSALYGSKHSPVFNEALTVM
                     HETYQVKSLQDAIRENPEEADYLRSYYSASLMLSRSVKRFYTNEQDYQKMIEDCQNEF
                     GSPTAFQIIQDGSWGEGLNAVLMESKHLAATAIQRFFRAAQALDQVNANQSTTDTKPS
                     EKPRLF"
     gene            complement(2267..4525)
                     /locus_tag="AC06IO_002456"
     CDS             complement(2267..4525)
                     /locus_tag="AC06IO_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HIO9875627.1"
                     /translation="MFAEIPKIQEQFHNVFEYSQSMASLWLQSIDKSLEQTNKVTQSF
                     INLNSQQVYPYPNILNDAIEYTADFMQRSILFWDIMRKRGNQYLKHEQEGQPPVLIFS
                     YNMLMDGRHFERPVNYALVEIIPPEGIQIDPAKRPYVIVDPRAGHGAGISGFKDASQV
                     GLALRAGHPVYVIIFFPVPEPNQTLVDVTAAHEKFLTEVALRHPQSPKPCLIGNCQGG
                     WAILTLMAANPDVAGVAVVNGAPLSYWGGENGKNPMRYIGGIWGGSWIAQMAGDLGNG
                     KFDGANLVMNFEMANPKVTYWSKYYNLFANLDKEEIRFLNFERWWGGFSLLNVNEMRG
                     IIDNLFIGNKLVHGKIPLGQSGNNLDLRNISVPVIIFCSEGDNITPPQQALNWVSDLY
                     SNTLEIKLDGQVIVYLIHKSVGHLGIFVSSEVAKKEHNQIIDLLNYIEHLAPGLYELK
                     LNETKENPKSPPHYLAYIEERSINDIRKGQESNVAIFNLVRMVSEYNAMSYDLFMAPI
                     IRNISNEYTAELIRKLHPLRQNQYLLSDLNPLVHPVSWISPFIRQNRIKISENNPFLT
                     QQLNFSKLINALWDFCSASRDNTVELMFYAVYGYIQLLAPFDPRRDMIVHSPEKDNQE
                     KITQSIIAHISDGGVPEAILRILLLLVKIQGYIIGANLPDVIQKLRECNALKHLDKNG
                     FKQIVHSQTIMIEHDPELAFNTIPHLLKSQEERSMVIQFIEDILKSLKVSPSEEYKKK
                     FQRIKELLQNQA"
     gene            4939..6387
                     /locus_tag="AC06IO_002457"
     CDS             4939..6387
                     /locus_tag="AC06IO_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875628.1"
                     /translation="MFSANQYLDFAWLSMQMFLINLLKDKIMRYAVTYCAMDHEFSGN
                     PFWHSCILLSQWDDNGKIEVVDNWGFYGVPSTVRDTWLSKLKIKLGLDVDLKGNHGML
                     RHEELRYLDVGYGLHGVTFELTREKFDLLQLKCQTMLEEQRQAVKEVVESQGIAGKQK
                     YRIYEHEEYSLLIFALEKIKATQKGHRPRLKPFELNLSFTLWGPALNQSYTCKSLMVD
                     LLKGILTQEQIARITEEGKHPTVPRYSGKLEKIYLHSSGPLREHKKSSGAIVHYRDLQ
                     DEGVKLHWTLPPQEIEALSGDTIKLLEISEEYCDEVKQAISKLQKLEWLFIDAKLPEK
                     YQSYQEDLIARIRQCYEAFAEVEPKKAKSTATGWMGFALSLFSLPRDLDEKILLQKLN
                     NAKYLINSLYMAIVDHWKIYDDWPSETKTDSTDYNPLEALAAYLCEEDKKKFCKIIGR
                     NYLDPSSEEEFDDIEESEWKNSELGSTSARTM"
     gene            6672..7793
                     /locus_tag="AC06IO_002458"
     CDS             6672..7793
                     /locus_tag="AC06IO_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIO9875629.1"
                     /translation="MNALTSWIKAWSQKFFPNQKKNENKEEEADLTQEKKVRPNADFY
                     ENYFSLVAAGTRLKLIEAMFGVNLFSLFENKVWVFEKDIIEKLQLMPIRARKWLHLLS
                     SEHFLIQIKVNGQQAYQLPEEFIELIHSEEFPAMEMFFKTWLVSTEENLTDVLRFGKV
                     KTSVSWPPKTQQEAEWLEDWMTRTSDQPTYCLLQSINFKKVNKLLDVGGGDGTMACTF
                     VKKYPHLKAAVYNLPMSAEMAKKNIESRKLNHRVHVIEGDFIEEDAFPLGYDLILFTR
                     VLFDWDDQVCRKLLRMAYQALPKNGLVGICEFYKEDNNDICLASEYRYIFHDDFGVNV
                     MKRASEYQNMLEKIGFTIVQPHKVKGMHFYYCSLILAQK"
BASE COUNT         2375 a         1328 c         1579 g         2532 t
ORIGIN      
        1 attgtttttt atgggaatcg ccacctcaat ttagcaaaaa gaattgcata catcacaatc
       61 aagtgtagtt tatttttctc aattaaatca gcaaaaatgg tcaaatattt tcagtaagaa
      121 agcaatattc ttattctgta aatcaagtta ataatcggaa acaagaaagt gaaatatcaa
      181 caaaaattgg agaatatgat tgatttcatt ggaaagcatc tcgatgaaga gctttctctc
      241 gaaagcttga gtgaaatttt ttgtatatca aaatttcact ttcatcgctt atttactgca
      301 tttaccgggt tgtctttgca gcagtatatt aaatggctac gcttaaaacg cgctgctcat
      361 cagcttgttg tcgagaaaga ccagtccgtc attaacattg caatcaatgc gggttttgaa
      421 tcccatgaag cgttttccag ggcttttaag aaatcttgtg gatttagccc aagtcagttt
      481 aggcaaggtt ttgggaggtc atattgggag cagccaccgt attgtttgcc tagacaaggt
      541 agaattgata tgaaagtaga tattaaaagt attgataaga tacgattagc tgtaattgag
      601 cacaaaggtg atcctaaatt gttaggtgaa agcattaaca agttagtgtc ctgggcaaaa
      661 tctcaatcga ttaatcttaa accacgaccc ggagaagcgt ttgctctggc ttatgatgac
      721 cctaaaacaa caccaccatc tgaatttcgt atcgatcttg gcatcaaggt ccccgaaaat
      781 ttgaaactgg atggtgtgat tgaaaagttt ctgccatcag gccgttatgc agtaacagta
      841 cataaaggtt ctcgtaataa tattggcgat gtggtttatt atctttatcg agattggtta
      901 cctaatacct cagaacaatt aggagattta ccctgtatct tctgttatta caattttgat
      961 catgaagtag ctgaaacgga acttttaaca gaatgttggc ttttgttaaa ataatccgct
     1021 ttatatgcca gtcggaatag ctggtaacaa taggccgggc ataacctgcg tctttttaag
     1081 ttctagttta aataacttgt caaacaatct atgtgcctca gcttgtctat gaggcattca
     1141 taacacattt tgtagatgct actgacaagc aatggtatgc agaaaatccg tcctttaaaa
     1201 ctcaagtaac tatacattaa atccagttac tcaatttatc ttcaaaagag tcttggtttt
     1261 tcagagggtt tagtgtcggt tgttgactga ttggcattca cttgatccag cgcttgtgcc
     1321 gcccgaaaaa atctctggat ggcagttgct gccaagtgtt tgctttccat caaaacagca
     1381 tttagtccct ctccccatga accatcttgt ataatttgaa atgcagtagg gctcccaaac
     1441 tcattttggc aatcttctat cattttttga taatcctgtt catttgtata aaatctttta
     1501 acagaacggg ataacataag agatgcggaa taataggaac gaaggtagtc tgcttcctcc
     1561 ggattttctc taatggcatc ttgtaatgat tttacttggt aagtttcatg catgacggtc
     1621 aatgcttcat tgaatactgg agagtgcttg ctgccataaa gcgcagaaac aacaaaccga
     1681 tgcttggcaa attctgcacc ataacttgct ttaggttgtg acaagaaagg ggtattttca
     1741 gtaatagcag gcaatttctc tataggaacg acatcagtat caaaataata cccgccatca
     1801 ttaaaaattt taaccttgcg aacaatattc gataaagtag cataggcttt ttccctgaga
     1861 gcacgctccg cttctctcag caaaaactct tgtcgaacat ccgtcaggtc aaaacattgt
     1921 ttccagtgaa tggtatttat tccgggaagc tgaatagaag aagaatgttc atcagtgatg
     1981 aaattgattt gatagtctgg gtttaaacta tgccattttt ttaaatgctg taaatatttg
     2041 tagggaaggc gttgtggtcc tacccaaatg atattaattt ctttagacat attgtgtcta
     2101 ccaccaatta gaaaaaaatt gcttttatag caacgcattt agaatgtcaa taaaataaac
     2161 aattgagatg ggggatttca tttgtctgaa agatgaaggg ttaattttcc aataggatat
     2221 ctataggttt aggcttgatt gagagcagta tggctcaatt cggaaatcaa gcctggtttt
     2281 gtaacagttc ttttattctt tgaaatttct ttttatattc ttcggaaggg gatactttta
     2341 aagattttaa tatatcttca atgaattgaa tgaccattga gcgttcttct tgtgatttca
     2401 gtaaatgagg tattgtatta aatgcaagtt caggatcatg ctctatcatt attgtttgtg
     2461 agtgcacaat ttgtttgaat ccatttttat ctaaatgttt aagggcattg cattcacgta
     2521 gtttttgaat gacatcaggt aagttagcgc caatgatata gccttgaatt ttcactaata
     2581 atagtaatat acgcaaaatg gcctctggta cacctccatc actaatatgg gcaataattg
     2641 attgagttat tttttcctga ttatcttttt caggggaatg aacaatcata tctcttctgg
     2701 gatcaaatgg ggctaataat tgaatgtagc catagacagc ataaaacatt agttccacag
     2761 tattatcacg agacgcgctg cagaaatccc ataatgcgtt tataagcttt gaaaaattta
     2821 attgctgagt taaaaaagga ttattttcag aaattttaat gcgattttga cgaataaaag
     2881 gtgatatcca tgatacaggg tgcactaatg gattcagatc actcaataaa tactgattct
     2941 gccttagtgg gtgtaatttt cgaatcaatt ctgcagtgta ttcgttactt atattacgga
     3001 ttatcggagc cataaacaag tcatagctca tggcattgta ttcagatacc atacgaacaa
     3061 ggttgaagat tgctacatta ctctcctgtc ctttcctgat gtcgttaata gagcgctctt
     3121 caatataagc gaggtagtgt gggggtgact tgggattttc ttttgtttca ttcagtttta
     3181 gttcatataa acccggagcc aaatgttcta tgtaatttaa taaatcaata atctggttat
     3241 gttctttttt agccacctca cttgagacaa aaatacctaa atgaccaaca ctcttatgga
     3301 taagataaac aataacttgt ccatccaatt tgatttccag agtatttgaa taaagatcag
     3361 aaacccagtt taaggcttgt tggggtggtg taatgttgtc gccttccgag cagaaaataa
     3421 taacaggcac actaatgttt cttaaatcca ggttattacc cgattgcccc aagggtattt
     3481 tgccatggac cagtttgtta ccaataaaca aattatcaat tattcctctc atttcgttca
     3541 cattcaaaag agaaaaacct ccccaccatc gctcaaaatt taaaaatcgg atttcttctt
     3601 tatcaagatt ggcaaagaga ttgtaatatt tgctccagta agtgacttta ggattagcca
     3661 tttcgaagtt cattaccaaa ttagccccgt caaatttgcc gttacccaaa tcaccagcca
     3721 tctgtgcgat ccagctccct ccccaaattc ctccgatgta gcgcatggga ttttttccat
     3781 tttccccacc ccaataggaa agaggagcgc cattgactac agcgacccct gctacatctg
     3841 ggttagcggc cataagggtt aaaatagccc atcctccctg acaattacca attaagcagg
     3901 gtttgggact ttgagggtga cgcaaggcta cttcagttaa aaatttttcg tgggctgcgg
     3961 tgacatccac cagcgtttga ttgggttcag gaacagggaa aaagataata acgtaaactg
     4021 ggtgtcctgc ccttaaggca agtcctactt gtgacgcgtc tttaaaaccg ctgattcctg
     4081 ctccatgacc agctcgcgga tcaacaatca cataaggtct ttttgcagga tcaatctgta
     4141 tgccttcagg gggaatgatt tccaccagtg cataatttac aggtctctca aaatgtctgc
     4201 catccatgag catattataa gaaaaaatga gaacaggagg ttgtccttct tgctcatgct
     4261 tgagatattg attgccccgt ttacgcatga tgtcccaaaa cagaatgctt ctctgcatga
     4321 agtcggcggt atattcgata gcatcattta atatattggg ataaggataa acctgttgcg
     4381 aatttaagtt gatgaaagat tgtgttacct tgtttgtttg ttcaaggctt ttatcaattg
     4441 attgtaacca aagagaagcc atggattggg aatattcaaa aacgttatga aattgttctt
     4501 gaattttggg tatttcagca aacatctttt tctcctgtga cttctcaatt cggggttctt
     4561 tacttccatc cgcgcttatt tttagccttt ttaacgcgtt ctggcttgtt ggtcagggtc
     4621 aaggggaggc attgtgcatc gatcatttaa atccatttgt taatcacttt taaatcatta
     4681 tagacagtta ttgttaaaac agtaaatgta ctttgtttgt tgaaggtcga ttgtggcgct
     4741 ttatattttt atcaattttg tccttaggtt gaacggccaa gcatttgttt ctgctaactc
     4801 ttatgttggg tggttggatt tgattttatt ggaaaatttc cagggattat ttcattatcc
     4861 gtttagccgt tgacgccatt gtggggatct gtaatagttt cataggattg attatgtgag
     4921 tcagtttcaa ccactttgct gttctcagct aatcaatacc tggattttgc ctggttatcc
     4981 atgcagatgt ttttaatcaa tcttcttaag gataaaataa tgagatatgc agttacctat
     5041 tgtgcaatgg atcatgagtt tagtggtaat cctttttggc atagttgcat tttattatct
     5101 caatgggatg acaatggaaa gattgaggta gtcgataatt ggggttttta tggggtgcca
     5161 tctactgttc gcgatacgtg gttaagtaaa ctgaaaatca agttgggttt ggatgttgat
     5221 ttgaagggta atcatggcat gttaagacat gaagaactgc gttatctgga tgttggctat
     5281 ggtttacatg gagttacttt tgagttaacc agggaaaaat ttgatttatt gcagttgaaa
     5341 tgccaaacaa tgttggaaga acagaggcaa gccgttaagg aagttgttga atcacagggg
     5401 attgctggca aacaaaaata ccgtatttat gagcatgaag agtattccct gttaattttt
     5461 gctttggaaa aaatcaaggc aacacaaaaa ggccatcggc caaggcttaa accatttgag
     5521 ttaaatttgt catttaccct gtgggggccg gctttaaatc aatcctatac ttgtaaatca
     5581 ctgatggttg atctgttaaa aggcatacta actcaagagc aaattgcccg aattactgaa
     5641 gaggggaaac accctaccgt accaagatac agtggtaaat tggaaaaaat ttatttgcat
     5701 agcagtggac cattacgtga acataaaaaa tcttccggtg cgattgttca ttatcgtgat
     5761 ttacaagacg agggagtcaa attacattgg acattacctc cacaagaaat tgaagcgtta
     5821 tccggagata ccatcaaatt actggaaata agtgaggagt attgtgatga ggtaaaacaa
     5881 gcgataagca agctgcaaaa attggagtgg ttatttattg atgccaaact gcctgagaaa
     5941 tatcagtctt atcaagaaga tttgattgct cgtattcggc aatgttatga ggcttttgct
     6001 gaggttgagc ctaaaaaggc taagtcaaca gccacaggct ggatgggatt cgccttgtca
     6061 ttgttctctt tgccaagaga tcttgatgaa aagattcttt tgcagaaatt aaacaatgct
     6121 aaatatctga ttaacagcct ttacatggct attgttgatc attggaaaat ttatgatgat
     6181 tggccatctg aaactaaaac agactctact gactataatc ctcttgaagc tttggctgct
     6241 tatttatgtg aagaggataa gaagaaattt tgtaagatta ttggacgaaa ttatctggat
     6301 ccttcatcag aagaggaatt tgatgatatt gaagagagcg aatggaagaa ttcagagtta
     6361 gggagcactt ctgccagaac catgtaaaaa agcctgactg acagttatct ttttaaagta
     6421 gcaagtgcgg actagcacgc tggatcatat ctgcatttgc atacttatcg ttcccattgt
     6481 gttatttgag gaaaccagcg gaacgagttt tttctacagg ctcccaatct ccttgttaaa
     6541 aaagagttta acttagtcat ttagtttatc taaaccccct cattaaaaac attttttgcc
     6601 ttgaccctgg ttttttttgt aagtcctgtt ctattctcat attaatagta ctcttaaaag
     6661 aatattgtgg gatgaacgct ttaacatcgt ggataaaggc atggtcacag aaatttttcc
     6721 ccaatcagaa aaaaaatgag aataaagagg aagaggctga tttaacccag gaaaaaaagg
     6781 tgaggcctaa tgccgatttt tatgaaaatt atttttctct tgttgccgct ggtaccagac
     6841 ttaaactgat tgaagccatg tttggtgtca atttattttc tttatttgaa aataaggtat
     6901 gggttttcga aaaggatatt atagaaaaat tgcaattaat gccaataagg gccaggaagt
     6961 ggctgcatct tcttagttcg gagcattttt taatacaaat taaggttaat gggcagcaag
     7021 cttatcaatt acccgaggaa ttcattgagt taatccacag tgaagaattc cctgccatgg
     7081 aaatgttttt taagacctgg cttgttagta cagaagaaaa tctgaccgat gtattgcgtt
     7141 ttggaaaagt caaaacgagt gtttcctggc ctccaaaaac tcagcaagaa gcagagtggc
     7201 ttgaggattg gatgactaga acatctgatc agcctaccta ttgtttatta caatctatca
     7261 attttaaaaa ggtcaataaa cttttggatg tgggtggtgg tgatggaact atggcctgta
     7321 cttttgttaa aaaatatcca catttaaaag cggcagtcta caatttgcca atgtctgcag
     7381 agatggcaaa aaaaaatatt gaatcgagga aactaaacca tagagtgcat gtgattgagg
     7441 gcgattttat tgaggaagat gcctttccac tagggtacga tctgatttta tttacaagag
     7501 ttctgtttga ttgggatgat caggtgtgta gaaaattatt aagaatggct tatcaggcct
     7561 tgccaaagaa tggattagtc ggtatttgtg aattttataa agaggataac aacgacattt
     7621 gccttgcctc ggaatatcgt tacatttttc acgatgattt tggtgtgaat gtgatgaaaa
     7681 gggcctcaga atatcagaac atgttagaaa aaattggttt cactattgtt caaccgcata
     7741 aagtaaaggg gatgcatttt tattattgtt ctttgatatt ggctcaaaag taaaaactct
     7801 tatctgtatt attg
//
LOCUS       DBNCWK010000136         7575 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.136, whole genome shotgun sequence.
ACCESSION   DBNCWK010000136 DBNCWK010000000
VERSION     DBNCWK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.136"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..277
                     /locus_tag="AC06IO_002459"
     CDS             <1..277
                     /locus_tag="AC06IO_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIO9875630.1"
                     /translation="KIYEIKCPDGEEVSLILAAEKLNEKMQNNKNKFKHLDNFHILLL
                     AALDIGHELIICKNEQEQQRLQVTQFISSLENKINKAVVGSEMDSNQ"
     gene            355..1926
                     /locus_tag="AC06IO_002460"
     CDS             355..1926
                     /locus_tag="AC06IO_002460"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIO9875631.1"
                     /translation="MRRQWYIGFGVILLCLFALFAMLQDIVWFFAFLIPIVILWIYDV
                     LQTKHTILRNFPVLGHVRYFLEFLRPEIQQYFIATDESELPFNRETRSMIYQRAKNVR
                     DTIPFGTERDILSVGYTWALHSLSPKHSSEVEPRIDVGGPDCKKPYNASRLNVSAMSF
                     GSLSANAIMALNKGAKLGGFYHNTGEGGLSPYHLQGGDIVFQIGTAYFGCRDDKGDFD
                     ENEFIKEASRDEVKMIEIKLSQGAKPSHGGILPAAKLTEEIAKVRKVPMGKDVLSPIA
                     HTAFDNPVGLLHFVKRLRDLSGGKPIGFKLCLGRRHEFMAICKAMLKTNILPDFITID
                     GAEGGTGAAPVEYTNFIGTPLEAGLVFVHNALVGTGVRDKIRVICSGKVTNGFDLLTN
                     IALGADICNSARAMMMSIGCIQSKQCNANTCPTGVATQKKRLQYGLVVDEKKFLVANF
                     HNNTIKSFLEMVGALGLNNPSDLKPSHIMRRIGVDEVKSFDQIYTYVKPAQFLQGDIP
                     DDYRIHWEHADPDKF"
     gene            complement(2018..3631)
                     /locus_tag="AC06IO_002461"
     CDS             complement(2018..3631)
                     /locus_tag="AC06IO_002461"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIO9875632.1"
                     /translation="MNIKSNLAHSSVILKGHVNRYAFLGLLISVGSILIASCIVSYQL
                     TGSVNLAGFIQAQRSNPAIWILDLTPFMFVYWGQAFCYGLVNKAESLLVDKTKELLNI
                     SGNLELKLKYESNHDSLTNLPNNRLLNEKIRLAIEQLGSQGELAVIVIKINDLNYNFS
                     SFNANNIVKQFAEKLKSILIDPYMLKASLGINMVARLQSDEFAILMPRLKKDLDINEL
                     LIHLLTLLNNNLMVDGISINVTSTVGAAIYPVHGEEDDVLVNHAIIAVYQARKGNKSY
                     AIYSPEMEEDLIQNRMVMNELQRSIENKDLKIYYQPIVELANGQIVGAESSARFEHPE
                     LGLLSAEKFMPLIEGTNLIHNLTTLMLKQVIKQLAAWHDAGHKIFVSVNLSVHHELPE
                     LIEKLLNDYEIAPQFLKLEFTERACLTEQMVTKEVFDRLSAMGIKLCIDDFCSESSSF
                     IQLTNFPIENIKIEKSFVLKIAKDPKKAKIVEAIVKLAQTLGWEPLADGIADQNALEK
                     LRDLGCLYGQGLYFSRAVNVAEFTSLLKK"
     gene            3828..3990
                     /gene="ssrS"
                     /locus_tag="AC06IO_002462"
     ncRNA           3828..3990
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC06IO_002462"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3985..5976)
                     /gene="uvrB"
                     /locus_tag="AC06IO_002463"
     CDS             complement(3985..5976)
                     /gene="uvrB"
                     /locus_tag="AC06IO_002463"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIO9875633.1"
                     /translation="MKDLFKIYSNYQPAGDQPTAIASLIDGLESGLAKQTLLGVTGSG
                     KTFTIAHVIQAMKRPTLIMAPNKTLAAQLYGEFKAFFPDNAVEYFVSYYDYYQPEAYV
                     PASDTFIEKDASINEHIEQMRLSATKALIERKDAIIVATVSAIYGLGDPDSYLRMLLH
                     LSRGEQSDQRKILKRLAEMQYTRTNLSLERGQFRVHGDVIDIFPADSEKEAIRIELFD
                     DEVDNIARFDPLTGEILQRLPRVTIFPKTHYVTPRERILETVEKVKVELQERLAELNA
                     QNKLVEAQRLEQRTCFDIEMMLELGYCSGIENYSRYLSNREAGEAPPTLFDYLPPEAL
                     LIIDESHVTVPQIGGMYRGDRARKETLVNYGFRLPSALDNRPLRFEEFEERSPQTIYI
                     SATPGPYEQEHSDNVAEQVVRPTGLIDPEVEIRPVKTQVDDLMSEIRQVIAQGSRILV
                     TTLTKRMAEDLTEYLSEHGIKVRYLHSDVDTVERIEIIRDLRLGEFDVLVGINLLREG
                     LDMPEVALVAILDADKEGFLRSERSLIQTIGRAARNVKGRAILYADNITGSMQRALTE
                     TERRREKQKAFNLKHGITPKGINKSVEDILEGAYIGKRKTMVAEQAPRYTHWSPQELA
                     KQINALEKQMYAHAQNMEFELAAKIRDEYLLLKEQLMKI"
     gene            6023..7201
                     /locus_tag="AC06IO_002464"
     CDS             6023..7201
                     /locus_tag="AC06IO_002464"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIO9875634.1"
                     /translation="MDIALAKRVQKVKPSPTLAVAAKAAQMKAQGLDIIGLGTGEPDF
                     DTPQHIKLAAISAIEAGDTKYTAVDGIVELKEAVKNKFKRDNELDYQLNQILVSVGGK
                     QSCYNLCQAYLNPGDEVIIPAPYWVSYPDMVLLADGVPVIIETTPAQRYKINAQQLEQ
                     AITPKTRMIFLNSPSNPSGIAYTQQELKELGEVLKKHPQILIATDDMYEHILWSQAFT
                     NILNACPELYDRTIVLNGVSKAYAMTGWRIGYAAGPAPLINAMKTIQSQSTSNPCSIA
                     QRAAVAALNGSNESIEEMVNAFHQRHDYVADRLQSIDGIEVIPADGTFYIFPSVQAII
                     EKRGYANDIEFSEKLLNEVGVALVPGSAFGTEGCIRISFATGIDTLKDALNRLQRFCS
                     "
     gene            7261..7336
                     /locus_tag="AC06IO_002465"
     tRNA            7261..7336
                     /locus_tag="AC06IO_002465"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7294..7296,aa:Asn,seq:gtt)
BASE COUNT         2305 a         1629 c         1458 g         2183 t
ORIGIN      
        1 taaaatctat gaaatcaaat gccctgatgg cgaagaagtg agtttaatac tcgcagcaga
       61 aaaacttaat gaaaaaatgc aaaataacaa aaataaattt aaacacctgg ataattttca
      121 catcctttta cttgctgctc tggacattgg ccatgaatta attatatgta aaaatgaaca
      181 ggagcaacag cgattgcagg tgacccaatt tatatcttct ttggaaaata aaattaataa
      241 agcagtagtt ggctctgaaa tggatagcaa tcaataagca gataaattaa gtattcctcg
      301 gcaaaccatc ctatactatt gatattaata gcaaacaatt aaaaggaatg agctatgcga
      361 cgtcaatggt atatcggatt tggagttatc cttttatgtc tctttgccct gtttgccatg
      421 ctgcaggata ttgtctggtt ttttgctttt ttgataccta ttgtaatcct gtggatttat
      481 gatgtattgc aaacaaaaca caccatttta cgcaattttc cagtactagg tcacgttaga
      541 tattttttgg aatttcttcg ccctgagatt caacaatatt tcatcgctac cgatgaaagc
      601 gagcttcctt ttaacagaga aacccgctcc atgatttatc aacgtgcaaa aaatgtcaga
      661 gacaccattc ctttcggaac ggaacgagac attctaagcg ttggctatac ttgggcactc
      721 cattctttat cgccgaaaca ctctagcgaa gtggaaccaa gaatcgatgt aggtgggcct
      781 gattgtaaaa aaccttataa tgcatcccga ttgaacgtat cggctatgag ctttggctct
      841 ttatcggcta atgccataat ggcattaaac aaaggagcaa aacttggagg attttaccac
      901 aatacagggg aaggagggtt aagcccatac catttgcaag gtggtgacat tgtttttcaa
      961 attggcacag cttattttgg atgccgtgat gacaaaggcg actttgacga aaatgaattt
     1021 atcaaagaag catcccgtga tgaagtaaaa atgatagaaa ttaaattatc acaaggcgcg
     1081 aagccatctc atggtggaat tttgcctgct gccaaattaa ctgaagaaat cgcaaaagta
     1141 agaaaagtac ccatgggcaa agatgtttta tcgccaattg cccataccgc ttttgataat
     1201 cccgttggtt tacttcattt tgtaaaacga ttacgcgatc tttccggtgg aaaacccata
     1261 ggctttaaac tctgccttgg gcgccgacat gaatttatgg ctatttgcaa agccatgctg
     1321 aaaacaaata ttttacctga ttttattaca atagatggtg ccgaaggagg gacaggagca
     1381 gcaccggttg aatacaccaa ttttatagga actcccttgg aagccggcct ggtttttgtt
     1441 cataatgccc ttgttggcac cggtgtacgc gataaaattc gcgtaatttg tagtgggaaa
     1501 gtaaccaacg gatttgattt gctcactaat attgcccttg gagctgatat ctgtaattca
     1561 gcaagagcca tgatgatgtc aataggatgt atacaatcga aacaatgcaa tgccaatacc
     1621 tgcccaacgg gagttgccac tcaaaagaaa cgtcttcagt atggtcttgt tgttgatgaa
     1681 aaaaaatttc ttgttgctaa tttccataat aatacgataa aaagcttttt ggaaatggtt
     1741 ggcgccttgg gattaaacaa tcccagtgat ttaaaaccat cccatattat gagacgaatc
     1801 ggggtagatg aagtcaaatc ttttgatcaa atctatactt acgttaagcc agcccaattt
     1861 ttacaaggcg atattcctga tgattataga atccactggg agcatgccga tccagacaag
     1921 ttttaatatc agtgtggcca tagcgtgtca gcgttgtgat tcgtattcca tgtgaatcaa
     1981 agcgggaaaa cgaatggtaa gtgatttaaa gccagattca tttctttagc aatgatgtaa
     2041 attcagccac attgaccgct cgagaaaaat ataatccctg accatacaag caacctaagt
     2101 ctcttaattt ttccaatgca ttttgatcag cgatgccatc tgccaggggc tcccacccta
     2161 atgtttgggc cagttttact atagcctcta caattttggc tttctttggg tcttttgcaa
     2221 ttttcagtac gaatgatttc tcaattttaa tgttttcgat agggaaatta gtcaactgaa
     2281 tgaaggaaga gctctcactg caaaaatcat caatgcataa tttgatgccc atggctgata
     2341 atcggtcaaa cacctctttg gtaaccatct gttcagttaa acaagccctt tcggtaaatt
     2401 caagtttcaa gaattgcgga gcaatctcat aatcatttaa taatttctct attaattcgg
     2461 gcaactcatg gtgcactgac agatttacag aaacaaaaat tttatgtcca gcatcatgcc
     2521 aggcggctaa ttgctttatg acttgcttaa gcataagagt tgtcaaatta tgaattaaat
     2581 tggttccctc gattaaaggc atgaattttt ctgcacttaa taaacccaat tcaggatgtt
     2641 caaaacgagc tgatgactcg gcacctacaa tttggccatt agccagctca actatcggct
     2701 ggtaataaat tttcaaatct ttgttttcaa tggagcgttg cagctcattc atcaccatcc
     2761 gattctgtat aaggtcctct tccatttcag ggctgtatat tgcataggat ttgtttccct
     2821 ttcgtgcctg gtaaactgca ataatggcat gattaactaa aacatcgtct tcctctccat
     2881 ggacaggata gatagcggca ccaacagtag atgtcacatt gatactaatt ccatccacca
     2941 tcaggttatt attcagcaag gtaagaagat ggattaatag ctcattaata tccaaatctt
     3001 ttttaagtct cggcatcaat atggcaaatt catcactttg caatcttgca accatattaa
     3061 ttccaagaga agcttttagc atgtagggat caatcaaaat cgattttaat ttttcagcaa
     3121 attgctttac gatattattg gcattaaatg agctgaaatt ataattaaga tcattaattt
     3181 ttatcacaat aacagctaat tcaccctgtg agcccaattg ttcgatggcg agccgtattt
     3241 tttcatttaa caagcgatta ttaggcaaat tggtcaagct atcgtgatta ctttcatatt
     3301 tgagctttaa ttccagattt ccactgatat ttaaaagctc tttggtttta tcaaccagca
     3361 aactctccgc tttgttaacc aatccataac aaaacgcttg cccccaatac acaaacataa
     3421 aaggtgttaa atccaggatc caaattgccg ggttagatct ttgagcctga ataaacccgg
     3481 ctaaattcac agacccagtc aattgatagg aaacgataca acttgcaatc aagatactgc
     3541 caacagaaat cagcaatcct aaaaaggcat atcgattaac atgacctttc aggataacag
     3601 agctatgtgc taaattagat ttaatattca tggattaaca gtaattccct tatgcctgtc
     3661 acaggctaat ttttaattcc tcatcccttc tacagcgact tcaatgctct acaaaaaact
     3721 gaataaatgg actacttaat gattagtata gcctaaagaa aaaataacaa atgctctatc
     3781 gtatggagtt tttctggatg taaaaaccag gttaatgtta taatctaatt aacccgatgg
     3841 cgagtgattt tagaaccaac actcacagcc agggaagcat cctgttatcg gcgttgagca
     3901 agcccaccaa tagagtggga agcctgaacc gatgcattcg ctgcccactt gaacctaagg
     3961 gttcaaaatc cggtacccat cgggttatat cttcatgagc tgctctttta atagcaggta
     4021 ttcatcccga attttagctg ccaattcaaa ttccatattt tgagcatgag catacatttg
     4081 tttttccagt gcattaatct gttttgccaa ttcctgagga gaccaatggg tatatcgggg
     4141 agcttgttca gcaaccattg tttttcgttt accgatatag gcaccctcca aaatatcctc
     4201 aactgattta ttaataccct tgggagtaat gccatgtttc aaattgaatg ccttttgttt
     4261 ttcacgtcgt ctctctgttt ccgtcaatgc tctttgcatc gaaccggtta tgttatcggc
     4321 atacaaaata gctcgcccct tcacattacg ggctgcacgg ccaatcgttt gaattaaaga
     4381 acgctccgat cgcaaaaacc cctccttgtc agcatccaaa atcgcaacaa gagcaacctc
     4441 gggcatatca agaccttctc gcaataaatt aatccctacc agaacatcaa actcacccaa
     4501 acgcaaatca cgaataattt cgatgcgctc aaccgtatcg acgtcggaat gcaaatagcg
     4561 caccttgatc ccgtgctcac tcagatactc tgtcaaatcc tctgccatgc gtttggttaa
     4621 tgtcgtgaca agaatacggc tgccctgagc aatcacctgt ctgatttcag acataagatc
     4681 atcgacctga gttttcaccg gcctgatttc cacctcagga tcaatcaatc cggtcggtct
     4741 taccacttgt tcagccacat tatccgaatg ttcctgttca taaggccctg gagttgctga
     4801 aatataaata gtctgcggcg aacgctcttc aaattcttca aaacgcaaag gcctattatc
     4861 caaggcagaa ggcaggcgaa atccataatt aaccaaggtt tccttgcgcg ccctgtctcc
     4921 gcgatacatc ccacctattt gaggaacagt aacatgcgat tcgtcgataa ttagcaaggc
     4981 ctccggcggg aggtaatcaa aaagagtagg cggtgcttcc ccggcttcgc ggttggataa
     5041 ataacgtgag taattttcaa tgccagagca ataacccaac tccaacatca tttcaatatc
     5101 aaaacaagtt ctttgttcca aacgctgagc ttctaccaac ttattttgag cattcaactc
     5161 agccagtctt tcttgtagtt ctacttttac tttttctact gtttctaaaa tacgttctct
     5221 gggagtaaca taatgagttt taggaaaaat agtgacacgg ggcaaccgct gtaaaatttc
     5281 tcctgttaaa ggatcaaagc gggcaatatt atcaacctca tcgtcaaaaa gctcaattct
     5341 tatcgcttct ttctcggaat cagccgggaa aatatcaatg acatcaccat gtacccggaa
     5401 ttgaccgcgc tcaaggctga gattggttcg tgtgtactgc atttctgcaa gacgctttaa
     5461 aattttacgc tgatcggatt gttctccacg ggagagatgt aataacatac gcagatagga
     5521 atccgggtcg cccaatccat aaatggcaga aaccgtcgct acaataatgg catctttgcg
     5581 ttcaatcaac gctttggttg ccgacaagcg catctgctct atatgttcat taatggaggc
     5641 atccttttct ataaaagtat ctgaagcagg cacatacgct tcgggctgat aataatcgta
     5701 gtaggaaacg aaatattcta cggcattatc aggaaagaag gctttaaact ctccataaag
     5761 ttgagccgca agcgttttgt tcggcgccat gatcaaagtg ggtcttttca tggcttgaat
     5821 aacatgagct atagtaaatg ttttaccaga accggtaacg cctaataatg tttgtttggc
     5881 taaacctgat tcaagaccat caatcaaaga ggctatggct gtaggttgat cgccggcagg
     5941 ttggtaattt gagtaaattt taaataagtc tttcatattt tatattatat gctagtaccc
     6001 ctataattca caggagcaac agatggatat cgcattggca aagcgtgtac aaaaagtaaa
     6061 accttcacct actcttgccg tagctgcaaa agcagcgcag atgaaagccc aggggcttga
     6121 tatcattggc cttggaactg gtgaacctga ttttgacacg ccgcagcata taaagctagc
     6181 cgccatctct gctattgaag caggtgatac caaatacacc gccgttgatg ggatagtcga
     6241 actaaaggaa gcggttaaaa acaaattcaa aagagacaat gaactggatt atcaactcaa
     6301 tcagatattg gtctccgtag ggggtaaaca aagctgctat aacctctgcc aagcttacct
     6361 taaccctggc gatgaagtca tcatccctgc tccttattgg gtatcctatc ctgacatggt
     6421 tttgctggcc gatggtgttc ctgtcatcat tgaaacgact cccgcgcagc gttataaaat
     6481 caatgcacaa caattggagc aagccatcac tccaaaaaca cgaatgattt tcctgaacag
     6541 cccttccaac ccctctggaa tagcctacac tcagcaggag ttaaaagaat tgggtgaggt
     6601 cttgaaaaaa catccacaga tactgattgc cacggatgat atgtacgaac atattctctg
     6661 gagtcaagct tttacaaata tactcaatgc ctgccctgaa ttatatgaca gaacgatcgt
     6721 attaaatggc gtgtccaaag cttatgccat gacaggctgg cggataggct atgcggcagg
     6781 ccctgcgcca ttaatcaatg cgatgaaaac cattcaatcc caatctacat ccaatccatg
     6841 ctccatagcc caaagagcgg ccgtagccgc tttgaatggc agcaatgaaa gcattgaaga
     6901 aatggtcaac gccttccatc aaaggcacga ctacgtggcc gatagattgc aaagcatcga
     6961 tggcatagag gtcatccccg ccgacggaac tttctacatt tttcccagtg tgcaggcgat
     7021 tatcgaaaaa cggggttacg ccaatgacat cgaattttcc gaaaaattgc tgaatgaagt
     7081 aggtgtagca ctggttccag gctccgcttt cggcacagaa ggctgcatca gaatatcctt
     7141 cgccaccggt attgacaccc tgaaagatgc cttgaaccga ctgcaacgtt tctgcagtta
     7201 aaaaatatat taaaaacctc ttgattctct cgcggaatcg ttttaagatt ccgcctcaat
     7261 tccccggtag ctcagtcggt agagcggatg actgttaatc attaggtcac aagttcgagt
     7321 cttgtccggg gagccaattc cagtctgggt tttggcgaat tccaagtttt cccttcctga
     7381 agcttggtac cagttttggt accgatttta aaatacaaaa taacgaaagt gtgggtattt
     7441 tccaattatg atcacaaccc taagaagaaa gctgttgaat ccctttttca aaagtaccgc
     7501 ctgataaagc attatttaaa tgaaagtaaa acaaatccct gtgtattttt tcaagttcaa
     7561 tcagaaatag ttttg
//
LOCUS       DBNCWK010000137         7467 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.137, whole genome shotgun sequence.
ACCESSION   DBNCWK010000137 DBNCWK010000000
VERSION     DBNCWK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.137"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            26..2617
                     /gene="pepN"
                     /locus_tag="AC06IO_002466"
     CDS             26..2617
                     /gene="pepN"
                     /locus_tag="AC06IO_002466"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIO9875635.1"
                     /translation="MPDTTIYLKNYQPPSFTVETVELNFDLYDDHALVNSTLKLTRQK
                     DGALHLYGEELELIALHLNGRPLEHEKYVLTKDALIIEQCPDEIVLNIVTRIKPQENT
                     QLSGLYRSNHLFCTQCEAEGFRRITYYLDRPDVLATFTTRITADKKQYPILLSNGNLM
                     DTGETADGRHWVIWEDPFKKPSYLFALVAGNLACVRDSFVTCSGRIVDLRIYVEPGNE
                     DKCSHAMESLKKAMKWDEEVYGREYDLDIYMIVAVSDFNMGAMENKGLNIFNSKYILA
                     RPETATDQDFADVEGVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSRDMNS
                     RDVNRIMDVKVLRSAQFPEDSGSMAHPVRPESYQEINNFYTATVYNKGAEVIRMQHTL
                     LGKERFRRGMDLYFKRHDGHAVTIDDFVAAMEDANGVDLTQFKLWYSQAGTPEVRVLS
                     HFSEGRLEITMQQNCPPTPECHEKKPFHIPVRIALFDLKGEMIPIENELLELKEKEQR
                     FIFSGLNEKPVVSLLREFSAPVKIYDDLDQDDLLFLLRYETDGYAKWDAAQRLVLNCL
                     NENLKLPASEWQVSKTLVAAFQHVLLDDSLDMDLRAELLTPPGFEEVAATMKWVDVSK
                     VESVRDYFRQQLGLGLYTEASDLYKELWEVEDHRMHGQAYGRRKLRNVCLWLMIKAKE
                     SDALELCREQFKDSRTMTDQIASCSLLVNCSRESHRNEAIEGFYKQWSHNELVLDKWF
                     AMQAACELPGTLDHVKKLTQHPAFCIKNPNKVRALVGAFCMANPRNFHALDGSGYAFL
                     SEVLIKLDKLNPQIAARLATPFTRWRSYDEPRQKLIQNQLEQLTKLDLSRDLREVVDK
                     SLIVEEK"
     gene            2969..3766
                     /gene="aroE"
                     /locus_tag="AC06IO_002467"
     CDS             2969..3766
                     /gene="aroE"
                     /locus_tag="AC06IO_002467"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIO9875636.1"
                     /translation="MLRRFAVIGNPIAHSLSPVIHQMFAQQTQIELIYEKILGDDVKF
                     EQQISDFFIQYGNGLNVTLPYKKRAYELAAVHTQRCTLAGAANTLWMKENQVHADNTD
                     GIGLIRDLSRFLELKDKKILILGAGGAARGIIFPLLEAKPLQLIVANRTLAKAEELQH
                     QFPQVNVKSLDELSESFDLIINATSASLSDQVIVLPEEVLSHKPFCYDLAYNQKTSTA
                     FVQYARNGGCEAVDGLGMLVEQAAEAFFIWNNIMPSTKEILEQLRSF"
     gene            complement(3816..4928)
                     /locus_tag="AC06IO_002468"
     CDS             complement(3816..4928)
                     /locus_tag="AC06IO_002468"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIO9875637.1"
                     /translation="MMVKKALYLAGGGARGAYQAGVLKAIGHILQTKTLPFDLVSGVS
                     VGSVNAAVLAENANDFPAGLEKLEAIWGEIHCQQIYKASNYELSKSVMRNLSTLIIKQ
                     RQSGHLLDTTPLRQFLEESIDFTRIEQNIQEGYLDTFEVLSACYETHQTISFYAHNHS
                     EFEDWNYPRHSSQRALINAQHILASSALPLFFPPVSLNGFHYGDGSIGLVAPLRGAIR
                     FQVDRILILGTRELPEFTDPETLRNGEIAFAHILGNMMNGLFLDNLDRDIEMVNRMNE
                     IATLLSMWKKRRSPWRPISTLHLRPSRAMASVAQSHYTSMPVLLRFLLNIMGARSHSG
                     DLLSFLLFEKEFTRELLELGYQDTIAASAEVSAFFS"
     gene            5066..6448
                     /locus_tag="AC06IO_002469"
     CDS             5066..6448
                     /locus_tag="AC06IO_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875638.1"
                     /translation="MGITKDERFRKFEIEKTLQLLQSMQRPIEYPLTPIDKTSLLKKA
                     TKRIPLLSKFLKTVDDTGEVVVKIGDRMTSLSHTPGKESLAHGFAFGGVAMAGFDFLR
                     IPVIYLSAYLLGEEVPVNLSNNAKWFYSALLLGLTITALAVPVTAPYIAFATAGLSLG
                     VGTFLLGKTLYERYQLGRERRKLKAEIIREEDEMLKIQDKAKHLESLLQSADNEEQIA
                     AILYEISVVQEDYNRQKEHIQSLKNDELQLEQQIKKLGTMKVVDRTIAVALSALTIAA
                     LAVTLFFPPVGGGMLAGIAIFGGAYFLARVSTPLIKATAVWLYNKLKSTPSESPESQD
                     IDNKPTLSNEKEEKKEDSLQIDNEFVLPPIDAEASISEDSKEYQPVHDSTEDVLVRLS
                     VNIDRLKEKVRTEKKEEIEEESHAELQHFSSLTPSPRTFGSRKLSAKENDDEDGESES
                     DIEHPDVPKV"
     gene            6516..>7467
                     /locus_tag="AC06IO_002470"
     CDS             6516..>7467
                     /locus_tag="AC06IO_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HIO9875639.1"
                     /translation="MLNLFREQPRAFHMIFMLELWERFGFYTVQGILTLYFIRYLGYS
                     DLESYYTFGAYSALVYGMVVIGGYLGDRILGTKRTIVLGLVVLAMGYFSLAVTDKQHV
                     FLALGLICVGNGLFKANPSNLLSKCYDDNDPRLHGGFTLYYMAINLGSMVALFVGPAV
                     SSAYGYSYAYMISAIGLILGIVNYWYQRQHVSHVNTKADLRKITAFHWGIIITGVLIL
                     TGISAYLLQHVMLAKNLLWGITLLVVSIYIYLMRKQAQASFMRMLVALVLMIEAVIFF
                     TLYQQMPTSLNLFAVNNVIPTFLGITIDPQSFQALNPIWIV"
BASE COUNT         2220 a         1315 c         1646 g         2286 t
ORIGIN      
        1 gtttttttta taggaagcaa tgactatgcc agacacgacc atttatttga agaattatca
       61 accgccttct tttacagttg agactgtgga attgaatttt gatttgtatg atgaccatgc
      121 tttagtgaac agcactctta agttaacacg tcaaaaggat ggcgctttgc atttgtatgg
      181 cgaagagctg gaattaattg ctttgcattt aaatgggcga ccattagaac atgagaagta
      241 tgtgttgacg aaagatgctc ttattataga gcaatgcccg gatgaaatag tacttaacat
      301 tgtgactcgc attaaacctc aagaaaatac gcaattatca ggactttatc gttctaacca
      361 tttattctgt actcaatgcg aagcagaagg tttcagacga attacctatt atcttgatag
      421 acctgatgtt ttggcaactt ttactacacg aattactgca gataaaaagc aatatccgat
      481 tttattgtct aacggtaatt taatggatac cggagaaacc gcggatggac gtcattgggt
      541 gatttgggag gatcctttca agaaaccatc ttatttattc gctttggtag cgggcaattt
      601 agcttgcgtt agagatagtt ttgttacttg ctccggcagg attgttgatt tacggattta
      661 tgtagaaccg gggaatgaag acaagtgctc tcatgccatg gagtcattaa aaaaagccat
      721 gaaatgggat gaagaggttt atggtcgtga gtacgattta gatatttata tgatagttgc
      781 ggtgagcgat ttcaatatgg gggcaatgga aaacaaaggg ttgaatatat ttaattccaa
      841 atacatcctc gctcgccctg aaaccgccac tgatcaagat ttcgcggatg ttgaaggtgt
      901 agtaggccat gagtatttcc ataactggac aggaaatcgt gtgacttgtc gcgattggtt
      961 ccagttaagt ttaaaagagg gattaactgt attccgggat caggagtttt ctcgggatat
     1021 gaattctcgt gatgtcaatc ggattatgga cgttaaggta ttgcgcagcg ctcagtttcc
     1081 cgaggactcc gggagcatgg cgcatccagt aaggccggaa tcctatcagg aaattaataa
     1141 tttttatacg gcgacagtat ataacaaagg cgcggaagtg attcgaatgc aacatacttt
     1201 gttaggcaag gagaggtttc ggcgaggcat ggatctttat ttcaaacgcc atgacggcca
     1261 tgccgtaacc attgatgatt ttgttgctgc catggaagat gccaatggag ttgacttaac
     1321 gcaattcaaa ctctggtata gccaggcagg gacacctgaa gtgcgcgttc tcagccattt
     1381 ttcagaagga cgattggaaa ttaccatgca acaaaattgt cctcctacgc ctgagtgcca
     1441 tgagaaaaag ccatttcata tcccggttcg cattgcatta tttgatttga aaggggaaat
     1501 gattcccata gaaaacgagc ttctggagtt aaaggaaaag gagcaacgtt ttattttttc
     1561 tgggctaaat gaaaagcctg ttgtctcact attaagagaa ttctcagccc ctgtgaaaat
     1621 ttatgatgat ctggatcagg atgacttgtt atttttattg cgttatgaaa cggatggata
     1681 tgccaagtgg gatgcggcac aacgcctggt tcttaactgt ctcaatgaaa acttgaaatt
     1741 gccagccagt gaatggcaag tctctaaaac tcttgttgct gcctttcagc atgttctgct
     1801 tgatgactct cttgatatgg atctcagggc agagttgttg acacctccag gatttgaaga
     1861 agtggcagct accatgaaat gggttgatgt cagcaaggtt gaatcagtgc gagattattt
     1921 ccgtcaacaa ttgggattgg gcttatacac agaggcgtct gatctttata aagaattatg
     1981 ggaagtagaa gatcatcgca tgcatggcca agcttatgga cgcagaaaat taagaaatgt
     2041 ttgtttatgg ttgatgataa aagcgaaaga atcagatgct ttagaattat gccgagaaca
     2101 attcaaggat tctcgtacca tgacggatca aattgcaagt tgcagcttgc tagtcaattg
     2161 ctccagggag tctcaccgaa atgaagccat agaaggattt tataaacaat ggtctcataa
     2221 tgaattggta ctggacaagt ggtttgcgat gcaagcggca tgtgagttac caggcacatt
     2281 ggatcatgtc aaaaaactaa ctcaacatcc cgctttttgt attaagaatc cgaataaagt
     2341 gcgggcgttg gttggcgctt tttgtatggc taaccccaga aacttccatg cgttggatgg
     2401 cagcggatat gcctttttaa gtgaggtatt gattaagctc gacaaactaa atcctcaaat
     2461 cgccgcacgc ttggctacgc cttttacgcg ttggcgaagt tatgatgaac caagacaaaa
     2521 gttaatacag aaccagttgg agcagcttac aaaactggat ttgtcgcggg atttgcgcga
     2581 ggttgtagat aagagtctga ttgttgagga aaaatagaat gaattaaagt caggacttac
     2641 tataaaccca atctctttgt taaaaaaagt ttttaattcg gtcatttagt ttatctaaac
     2701 tccctcatta aaaacatttt ttgcctcgac cttggggttt tttgtaagtc ctgaaagtgt
     2761 gctggatttt gctataggca gattcagcaa gacttaaacg atttttatcc ttttatttgt
     2821 gcaaacaaag cttacataat tcataaatta acttttcagc ttgattacct gggcgcgatt
     2881 taagaacaat ttggattgat gtgcttgttg cgtgagaaca gtttataatt aggaaaacca
     2941 atgaaactta taggataaag gagtttttgt gcttcgtcgt tttgcagtga taggtaatcc
     3001 aattgcacat agtttgtctc ctgtcattca tcaaatgttt gcccaacaaa cccaaattga
     3061 attgatttat gaaaaaatcc tgggtgatga tgtcaagttt gaacaacaaa tctctgattt
     3121 ttttatccag tatggcaacg ggcttaatgt cactttacct tataaaaaac gtgcttatga
     3181 attagctgca gtacacaccc agaggtgtac cttggctggt gcagcaaata ctctttggat
     3241 gaaagaaaac caagtgcatg ctgataatac cgatggtatt gggttgatac gtgatttatc
     3301 ccgatttctt gagttaaagg ataaaaaaat tctgattttg ggggctggcg gagctgcaag
     3361 agggattatt tttcctttat tggaagcgaa accgttacaa ttgattgttg ctaatcggac
     3421 tttggcaaaa gcggaagaat tacagcacca atttcctcaa gttaatgtca aaagtctaga
     3481 tgaattatcg gagtcctttg atttaatcat caatgctact tctgcgagtt tgtcagatca
     3541 ggtcattgta ttacctgaag aggtgttatc ccataaacca ttttgttatg atttggctta
     3601 taatcaaaaa acaagcaccg cctttgttca atatgctcgt aatggagggt gcgaagcagt
     3661 tgatggatta ggtatgttag tagagcaagc tgcggaagca ttctttatat ggaataatat
     3721 catgccttct accaaggaaa tcttagagca attgcgatcc ttttaaattg gacgcttgca
     3781 taacagttag atgtgctttg gcaaccaaac tctggctaac taaaaaaagc gctgacttct
     3841 gcggatgcag caatggtgtc ctgatatcct aattccagta attcccgagt aaattctttt
     3901 tcaaagagta aaaagctaag cagatcaccg gaatgactcc tggcccccat aatattcaat
     3961 aaaaaccgaa gcaatactgg catgctggtg taatggcttt gggctactga ggccattgcc
     4021 cggcttgggc gtaaatgcag ggttgagatt ggtctccagg gagagcgtcg ttttttccac
     4081 atggataaga gtgtcgctat ttcattcata cgattgacca tctcaatgtc tctatccaga
     4141 ttatccagaa aaagcccatt catcatattg cctagaatat gagcgaaagc aatttcacca
     4201 ttgcgcaagg tctctggatc agtaaactcg ggcaattccc tggtgcctaa gatgagaatt
     4261 ctatccactt gaaaacgaat agcaccgcga aggggggcga ctaaacctat acttccatca
     4321 ccataatgga atccattgag gctgacaggg ggaaagaata atggtaaggc gcttgatgcc
     4381 agaatatgct gggcattgat caaggcgcgt tggctgctat ggcggggata attccaatct
     4441 tcaaattctg agtgattatg agcgtagaaa gagatggttt gatgtgtttc ataacaggcg
     4501 cttaagactt cgaaagtatc gagatatcct tcttgtatat tttgttcaat tcgtgtgaaa
     4561 tcaatgcttt cttccagaaa ttgccgtaat ggtgttgtat caagcaaatg ccctgattga
     4621 cgttgcttga taatcaaggt gcttaaatta cgcattactg atttgcttaa ttcgtaatta
     4681 ctggccttgt agatttgttg acaatgaatt tcaccccaaa tggcttctag tttctccagg
     4741 ccagcaggaa aatcattggc attttcagca agtacggctg cattgacgct gcctacactg
     4801 acaccactca ctaagtcaaa aggtagggtt ttggtttgta aaatatgacc gatagctttg
     4861 agtacgcctg cttgataagc gcctcgagca ccaccacctg ccaaatatag agctttttta
     4921 accatcatgt tttattttat ttattataac ttgaaatata gttgaaatct gggctaatta
     4981 gcaagaaaga attatcgatg tcatcttttc ttaattatga attgatatac ttaaattaaa
     5041 tcgattcttt tcttgagtca ttatcatggg aattacaaaa gacgaacgct tcagaaaatt
     5101 tgaaatagaa aagactcttc aactcttaca gtcgatgcaa cgtcctattg aatatccgtt
     5161 gacgcctata gataaaacgt cattattaaa aaaagccaca aaacgcattc ctttgctgtc
     5221 aaaattttta aaaactgttg atgatacggg tgaagtagtt gtcaaaattg gtgataggat
     5281 gaccagttta tcccatacac ctggtaaaga gtctttggct catggttttg cctttggtgg
     5341 tgttgccatg gcaggctttg attttcttcg gattcctgtc atttatttgt cggcttattt
     5401 gctaggcgaa gaagtgcctg ttaatttgag taataatgcc aaatggtttt attccgccct
     5461 attgttagga ttgaccataa ccgcattggc agtcccggta acagctcctt atatagcttt
     5521 tgctacagca ggcctcagtt taggtgtggg tacgttttta ttaggaaaaa ctctttatga
     5581 acgctatcaa ttaggtcggg aaagaagaaa attaaaagca gagatcataa gggaagagga
     5641 tgaaatgctg aaaattcagg ataaagcaaa gcatttggaa tcccttttac aaagcgctga
     5701 caatgaagag caaatagcag ccattttata tgaaatatcg gttgtccagg aggactataa
     5761 caggcagaaa gaacacattc agagtttaaa aaatgacgaa ttgcaattag aacaacaaat
     5821 caaaaagttg ggtacgatga aagttgtgga caggactatc gcggttgctt tgtctgcgct
     5881 tactatcgcc gctttagctg tgactttatt tttcccgcca gttggaggag gtatgttggc
     5941 tgggattgcc atttttggcg gagcgtattt cttggctaga gtaagcacgc ctttaattaa
     6001 agcaacggct gtctggctct acaataaatt aaaatcgacc ccaagtgaga gtcctgaatc
     6061 acaggatata gataataaac cgactctaag taatgagaaa gaagaaaaaa aagaagattc
     6121 tttgcaaatt gataacgagt ttgttttacc accaatagat gctgaggcgt caatttctga
     6181 ggattcaaag gagtatcaac ctgtgcatga ctcaactgag gatgtactgg tgaggttatc
     6241 agtaaatatc gacaggctca aggaaaaggt aagaacggaa aaaaaagaag aaatagagga
     6301 agagtcccat gcggagcttc agcatttttc atctctaact ccctccccaa gaacatttgg
     6361 atccagaaaa ctatcagcaa aagagaatga tgatgaggat ggagaaagcg agtcagatat
     6421 agaacatcct gatgtcccta aagtttaaat ctgcattgca aaaaacaaga atttccttta
     6481 agttaaaaaa aattactatc aaggaatgaa aattcatgct gaatttattt cgtgagcaac
     6541 ctcgtgcatt tcacatgatt tttatgttgg aattatggga gcgatttggg ttctatacag
     6601 ttcagggaat tcttactctt tattttattc gctacctggg ttatagcgat ttagagtctt
     6661 actatacctt tggtgcttat tctgcgctgg tatatggaat ggtggttatt ggcggctatt
     6721 tgggagacag aattctagga accaagcgaa ccatcgttct cggtcttgtt gtgctggcca
     6781 tgggttactt ttctcttgca gttactgata agcagcatgt gtttcttgca ttaggattga
     6841 tttgtgtagg taatgggcta ttcaaagcga atccatcgaa tttgttatcc aaatgctatg
     6901 atgacaatga tccacgatta catggcggtt ttacccttta ttatatggct attaatctgg
     6961 gcagtatggt cgcattattc gtcgggccag cagtatcaag cgcttatggt tactcttatg
     7021 cttacatgat tagtgcaata ggtttgattt tgggtattgt gaattattgg tatcaacgac
     7081 aacatgtgtc tcatgtcaat acaaaagcgg atcttcgtaa aataacagcc tttcactggg
     7141 gaataataat aacgggtgtt ttaatactta ctggaatatc agcttattta ttacaacatg
     7201 tcatgcttgc caaaaatctg ttgtggggta ttactctctt ggtagtgtct atttatatct
     7261 atttaatgcg caaacaggct caggcttctt ttatgagaat gttagtcgct ttggttttaa
     7321 tgattgaagc agtcatattt tttactttat accaacagat gcctacttca ttaaatttgt
     7381 ttgctgtaaa taatgtaata cctactttcc tgggcatcac tattgatccg caaagctttc
     7441 aagctctcaa tccaatatgg attgtat
//
LOCUS       DBNCWK010000138         7356 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.138, whole genome shotgun sequence.
ACCESSION   DBNCWK010000138 DBNCWK010000000
VERSION     DBNCWK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.138"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..600)
                     /locus_tag="AC06IO_002471"
     CDS             complement(<1..600)
                     /locus_tag="AC06IO_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIO9875640.1"
                     /translation="MSKFIFVIGGTGTGKTTVSEEIKTIAEAQNFTVTILSLDHYYLP
                     QSMMDPDKPKNFDVPEALEQILIQQHLQDLEAGKTIFRPTYDMESSDRVLNGEMKIIP
                     ADIIIVEGIFAGEYLSSLRNQTEKLKIYLQSNQINDNYTRKEDRDIVERKRSSAYAHV
                     VKKNQMAGFFRYVAPHMISSNMVIDNPWLPQQNMTTTEKL"
     gene            complement(962..2116)
                     /gene="argE"
                     /locus_tag="AC06IO_002472"
     CDS             complement(962..2116)
                     /gene="argE"
                     /locus_tag="AC06IO_002472"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIO9875641.1"
                     /translation="MTTLDWLKQLISFDTTSRNSNLSLIEYLANGLNDFKVNPILIHD
                     SKEQKANLLATLPGKQGGLEGGIILSGHTDVVPVDGQIWDSDPFQATLKNNKVYGRGA
                     CDMKGFIAVVMALVPQLKEMNLDFPVHFAFSYDEEIGCLGVPSLIDKIVELNYQPRAC
                     IVGEPTLMKPVVGHKGKYSYRCQIHGVAAHSSLTNQGCNAIEHAASFISYLRGMANEF
                     KKYGNRDFSYDVPYTTLTTNLIKGGNAYNTIPNLCEFVFEFRNLSTDSSEDLNQKIMS
                     YVKEELVPNLHQDHPDSNVNLDTIAKAPGLDMPITDPFVRAAQTVCQSDKLMKVAYAT
                     EAGLFQQAEIPTIVCGPGSIEQAHRANEYVEVEQLRLCEEFIINMLRSHF"
     gene            complement(2128..3414)
                     /locus_tag="AC06IO_002473"
     CDS             complement(2128..3414)
                     /locus_tag="AC06IO_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875642.1"
                     /translation="MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHS
                     PVTSLLATMASSGVALVVRPFGGFILAVWADKYGHHKALMFVFYLMAVATMLITFTPS
                     YSTIGLAATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
                     LMGSSACLILTLYCSNETLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDT
                     GKLFILVKQQKRSLLIAFSLVAGCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGI
                     LVSILTIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQYPGVTILILVENILG
                     LCIGAYFGVLTITVSSLFPSSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMV
                     FTYYQMTTVFISLLAVLAYQEERKLSENQPEHLVAI"
     gene            3585..4316
                     /locus_tag="AC06IO_002474"
     CDS             3585..4316
                     /locus_tag="AC06IO_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIO9875643.1"
                     /translation="MKKFILFPLISALSSTFVHAGGMGDSNSCCSTFVSLEGGYTWSS
                     IDGYNFTIVGTNNTLTSTEDKQGYSGRLAVGVLSMIDDQYGFTGEVGWGYYGRTTINP
                     SVAGALGQVPAALTIKHTLSGFDALLGVTYFQNYFSWSAKAGALIQNMQVDTSAFFTP
                     QIFPIVDNFDMKTNRTAVLPAAKLGVAYNFDSNWAITGSYLFALGANPGTTATFNPNT
                     LRSSLSIDDENPMMNAILFGVQYTA"
     gene            complement(4469..5119)
                     /locus_tag="AC06IO_002475"
     CDS             complement(4469..5119)
                     /locus_tag="AC06IO_002475"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIO9875644.1"
                     /translation="MDRFQDRYQAGQLLGEKLKPYANRNDVIVLALPRGGVPVAAMIS
                     EAINAPLDLMIVRKLGVPGHEELAFGALAINKIKVYNEELLQYLQLSQKDINQVLSQE
                     ENELARRNRAYRGDKPFPDLKNKIVILVDDGIATGASMRAAVLALRKESPKKIIVAVP
                     VAPEDMNDIMKSVADEYICLISPMSFNAVGLWYEDFSQTEDEEVLYLLGKVYDRKV"
     gene            5451..5696
                     /locus_tag="AC06IO_002476"
     CDS             5451..5696
                     /locus_tag="AC06IO_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875645.1"
                     /translation="MNVHTMCFTRVLRYAVVTSLLFMAVSFTSVANAAQGCGEGYHRA
                     IHNGTCVLNYPGAFATPAPAHPGCWRNMWGQLRCYRY"
     gene            complement(5798..6835)
                     /locus_tag="AC06IO_002477"
     CDS             complement(5798..6835)
                     /locus_tag="AC06IO_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIO9875646.1"
                     /translation="MLFRHKGILYLFLSSLIISAANPVIAKLITLGNEHLVNGRNPIS
                     FCNVLFAGNFIAVLTLILIHFRELKEFQPEHLTAKNWCLIFLAALFAGFLTPTLFFFG
                     LMYTNVINVVLISNLQIPMTLLAGWIFLGEVPNIRVTTGAFLATLGVFAIVILQYWLG
                     GAGALTPNPEMKTGPLFIFLSSIPRSGEICIFLAVVSSTASTIIGFHALRRLPHYLFS
                     ILKMLLGLVLFFFIVISMFGWHHFADLFSPFLWEWMLFYGSVIIALRYYFSMLGMKHA
                     KVAEVAISSSIIPLMSIIFSYLILGEVPGISQIIGGSLILFGIYIALTGKLQAPEKTK
                     VLEKPSGFSGV"
     gene            complement(7204..>7356)
                     /locus_tag="AC06IO_002478"
     CDS             complement(7204..>7356)
                     /locus_tag="AC06IO_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HIO9875647.1"
                     /translation="PASLRQLAIGFFKEHPGHYTEKVAKKIPGDMMDEFTDIEPSSTK
                     PSSTGS"
BASE COUNT         2446 a         1444 c         1257 g         2209 t
ORIGIN      
        1 aagtttttcg gtagtagtca tgttttgttg aggaagccaa ggattatcaa tgaccatatt
       61 tgaagaaatc atatggggag cgacatatct aaaaaatcct gccatttggt tctttttcac
      121 aacatgggca taagccgatg atctttttct ttcaactata tctctgtctt ccttccttgt
      181 gtaattgtca tttatttgat tggattgtaa ataaattttg agtttttctg tttgattcct
      241 taaagaggat aaatactcac cagcaaaaat tccttcgaca ataatgatat cagcaggaat
      301 aattttcatt tccccattca atactctgtc actgctttcc atatcatagg tcggtctgaa
      361 tatggtttta ccagcttcta aatcctgcaa atgctgttga attaatattt gttccagagc
      421 ttcagggaca tcaaaattct ttggtttgtc cggatccatc atactttggg gcaagtagta
      481 atgatccaga cttaatatcg taaccgtgaa attttgtgct tctgctattg tcttaatttc
      541 ttctgaaaca gtcgtttttc cagttccagt tcctccaatg acaaagataa atttagacac
      601 gtaatattct cttgcggtca aaaaagccat tataataact gaggaacaaa ttacaagaga
      661 accgatcaat ctgtaccttg ataagccctc aagccaaatc tggcactcca aagcttacaa
      721 agcaaaccat tttaaagatt gtagatagta gataaaataa aatgattaca gcaaatgctg
      781 gcataaaaaa catttatttt aataaccaat tgataaaaaa gtattttttt taaaaccaat
      841 caaatttcat ctaatcatgt tatccacaaa aactgtgtat atctttgtga ataccttata
      901 aaagtgctag taaaatcagc atcaaatacc gatgattttt tacttaccaa tctgataaag
      961 ctcaaaaatg agagcgcagc atattgatta taaattcctc acataatcgt aattgctcta
     1021 cttcaacata ttcatttgct ctatgggctt gttcgatact acctggacca cacacaatag
     1081 taggaatttc tgcctgttga aatagcccag cttctgtagc gtaagctact ttcatcagct
     1141 tatcgctctg gcaaactgtt tgagctgctc tgacaaaagg atctgtaatc ggcatatcca
     1201 aaccaggagc ttttgctatc gtatcaagat taacattaga gtcaggatgg tcctgatgca
     1261 ggttagggac aagctcttcc ttaacataag acataatttt ctgatttaaa tcctcagagc
     1321 tgtcagtaga cagatttcta aattcaaaaa caaactcaca caagtttgga atagtattat
     1381 aagcattacc tcctttgatc agattggtgg taagtgttgt atatggaaca tcataagaaa
     1441 aatctcgatt tccatatttt ttaaattcat tagccatccc tcgaagatag cttataaaag
     1501 acgcggcatg ttcgatagca ttacatcctt gattcgttaa tgaagagtgg gcagctacac
     1561 catgtatttg gcagcgataa gaatatttgc ctttatgacc cacaacaggc ttcattaagg
     1621 ttggctcacc tacaatgcaa gcacgcggtt gataattaag ctccactatt ttatcgatca
     1681 gagaaggcac accaagacaa ccaatttcct catcataaga aaatgcaaaa tgaactggaa
     1741 aatccaggtt catttctttc aattgtggga ctaaagccat cacgactgca ataaatcctt
     1801 tcatgtcaca cgcgccacga ccatacactt tgttattttt aagagtggct tgaaaaggat
     1861 cactatccca aatttgaccg tcaacaggaa caacatcagt atgaccagac agaataatac
     1921 ccccttccaa acccccttgt tttcctggca aagtagctaa cagattagct ttctgctctt
     1981 tactatcgtg aattaggata ggattaacct tgaaatcatt taacccattc gccaaatatt
     2041 caatcaatga taaattagag ttccgcgaag tagtatcaaa agaaatgagt tgttttaacc
     2101 aatctaaagt tgtcatattc ctacctttta aattgcaacg agatgttctg gctgattctc
     2161 agatagcttc ctttcctcct gataagcaag gactgctagc aaactgataa agaccgtcgt
     2221 catttgataa taagtaaaaa ccatagggtt tttagtgtat tcaatcaacg ccgttataat
     2281 aaatggagca aaaccaccaa acaacaggac agctgaatta tagctgacgg ctagacaagt
     2341 agaacgtact gatgatggaa ataaggagct aaccgtaatg gtcaacactc caaaataagc
     2401 gccgatacac aaacctaaaa tgttttcaac caaaatgaga atagttacac cagggtattg
     2461 gttgagcaag aaaagtaaag gataaattaa tccaaaataa caagccaaag cacataacat
     2521 aactattttt ttgcctattc tatccgacaa tgctccaaaa aagggaatag taagaatact
     2581 gactaatatt ccagatgcag tgcctaagta cgcctctgtc agcgataaat tcaagtacaa
     2641 tttacaatag gtaggcatat agctaaataa ggtataaatc gagcctgtac acccggctac
     2701 aagactaaaa gctatcaaca gcgaacgttt ttgttgcttt accaagataa acagctttcc
     2761 tgtgtcctgt tgctctttct tccaggtaaa ttcccgttgc aatcgacgtc ttaaaatcca
     2821 tcctaaaggt agaatcaaca gtcctaaagc aaaaggtatt ctccaggcaa attgatatag
     2881 ggtttcattg gaacaataga gagttaaaat aagacacgct gaagatccca ttaatattgc
     2941 caaaagctgg cctaccattt gtaaggaagt gtaaaacccc tttttatcgc ttggcgataa
     3001 gctaactaat agagcgctgg atatgccaaa ctcgccacca gtagcaagcc cttgcaacaa
     3061 tcttgcaaat acaatgacac aagtagcagc cagacctatt gtggagtagg atggtgtaaa
     3121 agtaataagc atggttgcga cagccatcaa ataaaaaacg aacattaatg ctttatgatg
     3181 cccatattta tctgcccaaa ctgctaaaat aaaaccaccg aaaggccgga ctaccagagc
     3241 cactccggag cttgccattg tagccaagag ggaagtcaca ggactatgat tgggaaaaaa
     3301 agcttgggca ataaaaagca gcatgaagtt ataaatcatg aaatcatacc attccatagc
     3361 attaccaata catattgctc ccaatgtttt aaaggaggta tttgatgtat tcatgtttcc
     3421 ttacaagggt aaatttagag caaaacacat actagtagaa tataaaaaaa attaccatgc
     3481 tttataagaa actaatatca atattaattt tactggtaat tccctatcaa ctgatttaaa
     3541 ttattgactt ttacaataat aaacatggaa tgagttacta aatcatgaaa aaatttatcc
     3601 tgttcccttt aatttccgcc ctgtccagta cttttgtcca tgctggtggt atgggtgatt
     3661 ccaactcttg ttgctctacc tttgtgtcac ttgagggtgg atatacctgg agctccattg
     3721 atggatacaa ttttactata gtcggtacca acaatactct cacctcgaca gaagataaac
     3781 aaggttatag tggccgtctg gctgtcggtg tgcttagtat gatagatgat caatatggat
     3841 tcaccggcga agtaggctgg ggttactatg gtagaacgac cattaatcca agtgttgcag
     3901 gagctttagg ccaggttcca gctgccttga cgatcaagca tactttgagc ggatttgatg
     3961 ccctgctcgg cgttacctat tttcaaaact attttagctg gtctgccaag gcgggcgcgc
     4021 tcattcaaaa tatgcaagta gacactagtg cctttttcac tccccaaata tttcctatag
     4081 tggataattt cgatatgaaa acgaatagga ctgctgtttt gccagcagcg aagctgggag
     4141 ttgcctataa ctttgattct aactgggcta tcaccggctc ttatttgttc gcgcttggag
     4201 caaatccagg aacaactgca acttttaatc ctaacacatt aagatccagc ctgagcattg
     4261 acgatgaaaa tcccatgatg aatgctattt tatttggggt tcaatatacc gcttagttta
     4321 atattattta aaaacgtcac cttttatatg gggtcaagta tttggcccca agacttttta
     4381 acttattcct tcaacagatc ttcacagaac tcaaaaaaaa gataaaagct cctgtctttc
     4441 attttgccat gtttgtcatt tgttattttt agacttttct atcgtatact tttccaagta
     4501 aatataatac ttcttcatcc tcagtctgtg agaagtcttc ataccaaaga cccacagcgt
     4561 taaaagacat gggggatata agacaaatgt attcatccgc aacacttttc attatgtcat
     4621 tcatatcttc aggtgctaca ggaaccgcta ctataatttt tttaggtgac tccttacgta
     4681 aagcaagaac agctgcacgc atactagcgc ctgtggcaat accgtcatct accagaatta
     4741 cgattttatt ttttaaatca ggaaaaggtt tgtcaccacg ataagctcta ttccgccttg
     4801 ctaattcgtt ttcttcttga gaaagaacct gattaatgtc tttttgagaa agttgcaaat
     4861 attgcaacag ctcttcatta taaactttta ttttattaat ggccaaagca ccgaaagcta
     4921 attcttcatg cccaggaaca cccagtttgc gaacaatcat tagatctaat ggagcattta
     4981 tagcctcaga aatcattgca gctacaggca ctcctccacg gggtagtgca agcactatga
     5041 catcatttcg gttcgcgtaa ggcttaagct tctctcctaa taattgtcca gcttgataac
     5101 gatcttgaaa gcgatccatt ttcctactca attatctgtc ctgctatttt tattgtagtt
     5161 cagatctgga aattatcctg ctgtcagcaa gaatttataa tgattaatct tctctgaatt
     5221 taagctaata attatcagta cactatttca tcatttaaat ccatttcaaa taaagtgaat
     5281 agctgtgtac atcaaaatag tgcatcaagg aatataaaga atggattcat ttatagagtc
     5341 taaagatcac caaccaaaaa ttatgatcat attttaggca taatttaaaa attgactcta
     5401 tacttatgct gtatgctaaa aaataattga agcaatataa ggagagtaca atgaatgtgc
     5461 ataccatgtg ttttacgagg gtgttacgtt atgctgttgt gacaagcctt ctctttatgg
     5521 ctgtgtcttt tacatcagtg gcaaatgcgg ctcagggatg tggggaaggt tatcacaggg
     5581 ccattcataa tggtacttgt gttctaaact atccaggtgc ctttgcaacc ccggcaccag
     5641 cgcatccagg ttgttggcgt aatatgtggg gacaattacg ttgctatagg tactaattca
     5701 agtttacaaa tataaacata ttaaacctta ttttttgctg ttatatttct gatattacta
     5761 gcaaaaataa ggttttattc acagaaaaat agttgttcta aacgcctgag aaacctgaag
     5821 gcttttccaa aaccttggtt ttctcgggtg cctgcaattt tcctgttaaa gcaatatata
     5881 tcccgaacag aattaaactt ccgcctatga tttgtgaaat accgggaact tcacctaata
     5941 tcaaatagga aaaaataata gacatcagag gaattattga tgaactgatt gcaacctctg
     6001 caacttttgc atgtttcatg cccagcatac tgaaataata tcgcaaagca ataataacac
     6061 tcccataaaa gagcatccac tcccataaaa atggggagaa aagatcagca aaatgatgcc
     6121 aaccaaacat agaaatcact ataaaaaaga ataaaacaag ccccaataac atttttaaaa
     6181 tactaaacag ataatggggt aatcttctaa gtgcatgaaa accaataata gttgaagcag
     6241 tactggaaac tactgccaaa aaaatacaaa tttcacctga acgaggaata gagctgagaa
     6301 aaataaaaag aggacctgtt ttcatttcag ggtttggagt taaagcacct gctcccccca
     6361 accaatattg aagaataact attgcaaata ctcccaaggt agctaaaaaa gcccctgttg
     6421 ttactcgaat attaggcacc tctcccaaga aaatccagcc ggctaataaa gtcataggga
     6481 tctgtagatt ggaaataaga acaacattaa tcacattggt gtacattaat ccaaaaaaaa
     6541 ataaggtggg cgttaaaaaa cctgcgaata aagcagccag aaaaattaaa caccaatttt
     6601 tcgctgttaa atgttctggt tgaaattctt ttagctctct aaaatgaatt aaaatcaaag
     6661 tgagcactgc aatgaagttt ccggcaaata ggacattaca aaacgaaatt ggattacgac
     6721 cattcaccaa atgctcattc ccaagagtga taagttttgc tatgacaggg ttagcggcac
     6781 ttattatgag cgaactcagg aataaatata gtattccctt atgtcgaaac agcatcttaa
     6841 ttcccccctc acatccttat caaggcttta atcttgtaca ctcaatttta cctgtatcta
     6901 catctttttt caggtgagag acagcaataa atagtatatc ttgattagct ctccctgatg
     6961 cacaatcaaa tggcatggat agagtatgca cgatttgaat aatcttatct tgccaatcaa
     7021 tacataaatt tctttagatt tcttaagtgc agctaaaaat ttactttata aatctccctt
     7081 acagcacaat acggtccaat aagagaaaag aaaaggaaat gatacaatta aatccacgtt
     7141 tatttacaag ataaaaaacc acagtttcta tttatccgtt gctcgtctat gctcaataaa
     7201 aagttagctg ccggttgacg atggtttagt ggaagatggt tcaatatcag taaactcatc
     7261 catcatatct cctggtattt ttttggcaac tttttctgta taatgaccag gatgttcttt
     7321 aaaaaatcca attgctaatt gacgcaaact tgcagg
//
LOCUS       DBNCWK010000139         7331 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.139, whole genome shotgun sequence.
ACCESSION   DBNCWK010000139 DBNCWK010000000
VERSION     DBNCWK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.139"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            26..544
                     /gene="ankB"
                     /locus_tag="AC06IO_002479"
                     /gene_synonym="legAU13"
     CDS             26..544
                     /gene="ankB"
                     /locus_tag="AC06IO_002479"
                     /gene_synonym="legAU13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box/ankyrin repeat-containing T4SS effector
                     AnkB/LegAU13/Ceg27"
                     /protein_id="HIO9875648.1"
                     /translation="MKKNFFSDLPEETIVNTLSFLKANTLGRIAQTCQFFNRLANDKH
                     LELHQLRQQRIKRELWGNLMVAARSNNLEEVKKILKKGIDPAQTNSYHLNRTPLLAAI
                     EGKAYQTANYLWRKYTFDPNFKDNYGDSPISLLKKQLANPAFKDKEKKQIRALIREMQ
                     EEKIAQSKCLVC"
     gene            complement(575..994)
                     /locus_tag="AC06IO_002480"
     CDS             complement(575..994)
                     /locus_tag="AC06IO_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIO9875649.1"
                     /translation="MRAKNDECTHVLLVDDDEIDLMSIQRALTKLNIPVCFHIARDGV
                     EALEQLSGNIKATKPKIIVLDIMMPKMNGIEFLRELRSHIHYNPIKVVIFTTSSNIRD
                     KQATTGLDIAGYFVKDSQFNEFVYCCKHLLEKKSHLS"
     gene            complement(984..3434)
                     /locus_tag="AC06IO_002481"
     CDS             complement(984..3434)
                     /locus_tag="AC06IO_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9875650.1"
                     /translation="MRRENTKSNIDFKSIFDSLPDLYLLLDRDFNIVGVSDAYLQATM
                     VSRKQILGKNVFDAFPDNPNDPKATGAKNLYASLLRVLKNKSADTMAIQKYDIQRPAD
                     RGGGFEERYWSPINTPVLGADQEVEYIIHRVEDVTEFVHLKKTGSAQLKLMEELRTKA
                     GEMEIEIYQRAQEIQEANKQLEEANKNLARLDQIKTQFFANVSHELRTPLMLILGPLE
                     MLLAEKKLPSFVQNTLRRIQNNAQILLKHVNDLLNISKLDAGKMILHYANIDLVKLIQ
                     QTIALFEAQIPERKLVFSMDLPDELQAQIDPDKIQHILINILSNAIKFTPQNAKVRIR
                     LVQINPETCRLQIEDNGPGIPSDLCETIFERFFQIEEPTTRTVGGTGLGLSIAKDFVE
                     LHGGSILAGNARNGGAIFTIELPLKAPQNVKVYSSYSQLLLGEKEISVSLAEFRNKVK
                     PIKEGAEEDFEQDYYDRPLILVVEDNQEMNHFICKILNTFYRTESAFDGREGLQKSLA
                     LHPDLIVSDIMMPHMSGVEMVHAIREHPLLLKTPIIILTAKADDELTARMLEEGAQDY
                     VIKPFSAKEFKARVANLILVKKAEDELDQFVYHASHDLKAPLPAMEHLISWIEEDLGE
                     MLSEQSKKYLLLLRQRASRMSKLLDGLLKYAQSGYVFDKVRIIHTDRLITDIIKKLNP
                     PQTVTFQLEDTLPVLNTCELPLREVFTALLDNSIRHHHRKDGHIIIGTKEKDRFYEFF
                     VADDGPGIEKKYQDRIFQLFQTLQPRDKLETSGVGLSIAKKIVEFQGGKISVESEKNK
                     GSVFRFLWPKDKEIGDAC"
     gene            3846..5294
                     /gene="mavC"
                     /locus_tag="AC06IO_002482"
     CDS             3846..5294
                     /gene="mavC"
                     /locus_tag="AC06IO_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavC"
                     /protein_id="HIO9875651.1"
                     /translation="MTTSKLEKTGLHVHEKIKHMVKSYGTMITGIPAEILGQNEAEIS
                     VGYVKKMGNVKENIAEVVRKSEMTQPTNSCGKASNEVCDLLLGTEGASEFEKSSYQVL
                     SGDGSNLKGSLPNKNLLVRVEMDRFNAPQKYQKIKREEFNPETAEKNKIYLLEDQLVY
                     LDIFGKVIDLGQTSDTCHRLFNAITTPFYQNYILYDEYIDPEESAEEAAMFEMGEIVT
                     AKMKNIDCWTATHSFTIFVPEAESEETRTLYPYQAYWTSHTLQQWFSGDKDEKLSRLG
                     IDGYIEKLALLGTTTDSKIRSSIYGELFSPPGKERVFCTGMNEKFSPLRVKFKVTEVN
                     PEIALQNLQEVQEFIDTNYPGENAKAQCELYKTKAQEAMTQQLEMRLLTEPTHRKESV
                     TPYKEKNTQSFFSKNLDTTSRIDKMISSVITMENLKILAKEADTLSGKDREKLVEYFK
                     TLPSEQLAEIKPESETAQKAWDEQYGLVFRDK"
     gene            5370..6653
                     /locus_tag="AC06IO_002483"
     CDS             5370..6653
                     /locus_tag="AC06IO_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2148 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875652.1"
                     /translation="MIVRGINMTKIKLESPGFMVHKKLKSMSQNYGVMMTGVPAEVLG
                     QMQAERSIPSINKTGNLKQQIAKEVSKVCHMMTEPTQSCGQASNDVCELLLGKIEAEK
                     FHFTKYETLSADGDNLKNVLENTAPSSTNLLIRFEIDREDPPIVLVKTKNENFNPETA
                     VRNKIYLLENKLYFLDKIGNLFNLGPGKKKCTQLFNAIGDSAEYSLCDPFVLEEPDKP
                     EDFAISEIVDIFNKQKERFDFWIGSHSFTIYIPQTLGESPRQFYPYQAYFGSHTLQDW
                     FVSDKDEYLSRIGIDKYIEKLAVLGKTTNTKERSDIYAEFFSKRGREAFFCAHLNEKR
                     QPLRVKFKITEINPELALKNLQETQEFIDTHPGDNPSDKVENYRNRAKLAMTEHLESL
                     LDINPESSPSLLFWKNVDKKEQEMTDSSRYKKGPS"
     gene            6741..7100
                     /locus_tag="AC06IO_002484"
     CDS             6741..7100
                     /locus_tag="AC06IO_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875653.1"
                     /translation="MMSREKAAGTFFKDYQKKNVMRLLQESLEKIINEWLKTDDESHT
                     KLRSLQELSEMDINATSFAEHSPLPDFVTRLWLDPHKALDALDQKISKSEIRKLIKET
                     ARETELVFTHQKAPPYS"
BASE COUNT         2478 a         1396 c         1318 g         2139 t
ORIGIN      
        1 cttttaacca tcactatttg ctgctatgaa aaagaatttt ttttctgatc ttcctgagga
       61 aacaattgtc aatacattga gtttcttaaa agcaaacacc ctaggtcgta tagctcagac
      121 atgccaattt tttaatcgct tggctaatga taaacatctg gaactgcatc aactaagaca
      181 acagcgtata aagcgagagc tatggggaaa tcttatggtg gcggcaagaa gcaataacct
      241 ggaagaggtc aaaaagattc taaaaaaagg aatcgatcca gcccagacca atagctatca
      301 cttaaataga acacctttac ttgcagctat cgaagggaaa gcatatcaaa ctgcaaatta
      361 cctctggaga aaatacactt tcgatcccaa ttttaaagat aactatggtg attcacctat
      421 ctctcttctt aaaaagcaac tggcaaatcc agccttcaag gataaggaaa aaaaacaaat
      481 acgcgcctta atcagggaaa tgcaagaaga aaaaatagca cagagcaagt gccttgtttg
      541 ttaaagcttt taaattaaac agatcgggtc tggtctatga tagatgtgat tttttttcca
      601 gcaagtgttt gcagcaataa acaaactcat taaattgaga gtcttttaca aaatatcctg
      661 ctatatccaa acctgttgtt gcctgcttat cccggatatt acttgatgtc gtgaagataa
      721 caaccttaat cggattataa tgaatatgag aacgcaattc tctgagaaat tctataccat
      781 tcatttttgg catcatgata tcaaggacga tgattttagg cttggtcgct ttgatatttc
      841 cagagagttg ttcgagagct tcaacaccat cacgagctat atgaaaacaa accggaatat
      901 tcagttttgt cagcgctctt tgaatagaca ttaaatcgat ttcatcatca tcgaccaata
      961 aaacatgggt acactcatca ttcttagcac gcatcgccaa tctccttatc ttttggccat
     1021 aaaaaacgaa agacgctgcc tttgtttttt tctgattcca cagaaatttt acctccttga
     1081 aactcgacta ttttcttagc aatgcttaga cctacaccac ttgtttcaag tttgtccctg
     1141 ggttgtaagg tctgaaataa ttgaaaaatt ctgtcttgat attttttttc tattccaggg
     1201 ccatcatctg ctacaaaaaa ctcataaaac cgatcctttt cttttgtacc aatgatgata
     1261 tgtccatctt tacggtgatg atgcctgata ctgttatcga gaagcgccgt aaagacctct
     1321 ctcaaaggaa gctcgcaagt attcaatact ggtaatgtat cttcgagttg aaacgtgacg
     1381 gtctgcggag gattcaattt ctttatgata tcagtaatca atctatcagt gtgaattata
     1441 cgaaccttat caaaaacata tcctgattga gcgtacttaa gcaatccatc gagcaacttt
     1501 gacattcgtg aagcacgttg acgcaagagc agtaaatatt ttttagattg ttcagaaagc
     1561 atttcaccca gatcttcttc aatccatgag atcaaatgct ccatagcagg cagaggcgct
     1621 tttaaatcat gcgatgcgtg atagacaaat tgatccagct catcttcagc tttcttcacc
     1681 aaaatcaaat tagcaactct ggctttaaat tccttggccg agaatggttt tatcacataa
     1741 tcctgagccc cttcttccag catacgtgca gttaattcat cgtctgcttt ggcagttaaa
     1801 ataataattg gagttttcaa tagcaggggg tgttctctaa tagcatgcac catttcgaca
     1861 ccgctcatat gaggcatcat aatatcacta acaatgagat ctggatgtaa agcgagcgat
     1921 ttttgcaaac cctcccgacc atcaaaagca ctttcagtac gataaaaagt gttcaatatt
     1981 ttacaaataa aatgattcat ctcctgattg tcttctacta ccaggatcag cggtctatcg
     2041 taataatcct gttcaaaatc ctcttcagct ccctccttaa ttggttttac tttatttctg
     2101 aattcagcaa gactcacgct tatttctttt tcccccaata atagctggct ataggaagaa
     2161 taaaccttta cattttgagg cgcttttagt ggtaactcaa tggtaaaaat ggcaccgcca
     2221 tttcgagcat tccctgccaa aattgaaccc ccatgtaatt caacgaaatc ttttgcaatc
     2281 gataaaccca acccagttcc ccctactgta cgagttgtag gttcctcaat ctgaaaaaaa
     2341 cgctcgaata tagtttcaca aagatcagaa ggaatacccg gcccattatc ttctatctga
     2401 agcctgcatg tttcaggatt gatttgtact aatctaatcc ttactttcgc attttgtggt
     2461 gtgaatttta tggcatttga caaaatattg attaaaatat gctggatttt gtcgggatct
     2521 atttgtgctt gcaattcatc gggcaaatcc attgaaaaaa caagcttgcg ttcgggaatc
     2581 tgagcctcaa acaatgcaat tgtctgttga attaatttta ccaaatcaat attggcataa
     2641 tgaagaatca ttttgccagc atccagcttt gatatattaa gcaaatcatt gacatgtttt
     2701 agtaaaattt gagcattatt ttgtatgcgc cttaatgtat tctgaacaaa tgagggaagc
     2761 tttttttcag caagcaacat ttccagagga cctagtatca gcattaatgg ggtacgaagt
     2821 tcatgactga cattagcgaa aaattgggtt ttgatttgat caagtctagc tagattttta
     2881 ttagcctctt ccaactgctt gtttgcttcc tgaatttcct gggcacgctg atagatttca
     2941 atctccattt ccccagcctt ggtacgcagt tcctccatca gcttcaactg cgctgaaccg
     3001 gtttttttta aatggacaaa ttcagtaaca tcctccacac gatgaataat atattccact
     3061 tcctgatcag cccctaaaac cggagtatta ataggactcc aataacgttc ttcaaatcct
     3121 cctcctcggt ctgcaggtct ctgaatatca tatttttgta tagccattgt atctgctgat
     3181 ttgtttttta gaacccttag cagcgatgca tataggttct tagcacctgt tgcctttgga
     3241 tcgtttggat tatcaggaaa agcatcaaag acattttttc ccaaaatttg tttgcgactc
     3301 accattgttg cttgtaagta ggcatcactg actccaacga tattaaaatc cctgtccaat
     3361 aaaaggtata aatccggcag ggaatcaaat atagatttaa aatctatatt tgatttcgta
     3421 ttttcccttc gcacagagca tcctccttcc tctatgtaag gcctattgac tataaagcat
     3481 gacgcaagtc tttgcgaggc tggccatgaa tccaatgtgc aatttttaac acagggtatg
     3541 aatagccaat taaccctact actaattata atcgaggatt tgagacctta ctaattagga
     3601 aagcctggtt tcgataattg cttgcaacca ccaacaaaaa gatgagcctc tttcagccag
     3661 tacaacgctt gctttagtgg ttcaatcccc tctcagcaat ttttcttcaa tagtcgccct
     3721 atcgatttaa gatcttatcg tcaataaaac ataaaataaa tacaaaataa tcgtacgaaa
     3781 accaaagtgt acaaagttta ttttaatcta caattaaata tagggtatct atcagtgaga
     3841 ttaatatgac aacttccaag cttgaaaaaa cgggtttgca tgttcatgaa aaaatcaaac
     3901 atatggtcaa gagttatgga acgatgataa ccggtattcc tgccgaaata ttaggacaaa
     3961 atgaggcaga aatttcggtt ggatatgtaa aaaaaatggg gaatgtgaaa gaaaacattg
     4021 ctgaagtagt tcgaaaatct gaaatgactc aacccacaaa cagctgtgga aaagcgagca
     4081 atgaagtgtg tgatttactt ttaggaacag aaggagcgag cgagttcgaa aaatcaagct
     4141 atcaggtctt atctggggat ggcagtaatt tgaaaggctc tctacccaat aaaaatttac
     4201 tggtcagggt tgaaatggat cgatttaatg ctcctcaaaa ataccaaaaa ataaaaagag
     4261 aggagtttaa tcccgagacc gcggaaaaaa ataaaattta tctactggaa gatcagcttg
     4321 tatatctcga tatatttgga aaagtcattg atctggggca aacatcagac acatgtcata
     4381 gactatttaa tgctattacc accccttttt atcaaaatta tattctttat gatgagtata
     4441 ttgatccgga agaaagtgct gaggaagcag ccatgtttga aatgggtgag atagttacag
     4501 ctaaaatgaa aaacatagat tgttggacag caacccattc ttttactata tttgttcctg
     4561 aagcagagag tgaggagaca cgcacattgt atccttatca agcttattgg accagccata
     4621 ctttgcaaca gtggttttcc ggtgataaag atgaaaaatt atcacgcttg ggtattgatg
     4681 gctatatcga aaaactggct ttgttaggca caacaaccga tagcaaaata cgctccagca
     4741 tttatggaga actattttct cctccaggaa aagaacgtgt tttttgcaca gggatgaacg
     4801 aaaaattctc gccattacga gtcaaattta aagtcactga agtcaatcca gaaatagcat
     4861 tgcaaaattt acaagaagtt caagagttta ttgatacgaa ttatcctggc gaaaacgcga
     4921 aagctcaatg cgaactttat aaaacaaaag cgcaagaagc gatgactcaa caacttgaga
     4981 tgcgacttct aacagagccg actcatagaa aagaatcagt aaccccctat aaagaaaaaa
     5041 acacacaatc tttctttagc aaaaacttag atacaaccag cagaatagat aaaatgatct
     5101 catcagtcat tacgatggaa aacctcaaga ttcttgcaaa agaagcagat accctctctg
     5161 gaaaggatcg tgaaaagtta gtggagtatt ttaaaacatt accatcagag cagctggcag
     5221 aaatcaagcc tgaatcagaa acagctcaaa aagcctggga tgagcaatac ggattagttt
     5281 ttcgtgataa gtgatagatc gaatcggctt tgtgtatttg tcacttgaac tgtttataat
     5341 caaatgatat tgacctataa tatatcactt tgattgtacg tggcattaat atgacaaaaa
     5401 taaaactgga atcgccgggt ttcatggtac acaaaaagtt gaaaagcatg tcccaaaact
     5461 atggagtcat gatgactggt gtccctgctg aggtgttagg acaaatgcag gcagagcgat
     5521 ctatccccag cattaataaa acagggaacc ttaagcaaca aattgcaaaa gaggtttcaa
     5581 aggtatgtca tatgatgact gaacccactc agagttgtgg ccaagccagt aatgatgtgt
     5641 gtgaattgct tttaggaaaa attgaagcgg aaaaattcca tttcaccaaa tatgaaacat
     5701 tatcagcaga tggagacaat ctaaaaaatg ttttggaaaa tacagctcca tccagcacta
     5761 acctattaat acgatttgaa attgacagag aagaccctcc cattgtattg gtcaaaacaa
     5821 aaaacgagaa tttcaatccg gaaaccgctg tgaggaataa aatttaccta ttagaaaata
     5881 aattatattt tcttgacaag ataggtaatc tttttaacct tggaccaggt aaaaaaaaat
     5941 gtacgcagtt attcaacgca attggagact cagcagaata tagtctttgt gatccttttg
     6001 ttcttgaaga gcctgataaa cctgaagatt ttgcaataag tgagatcgtc gatatcttta
     6061 acaaacaaaa agagagattc gatttttgga tcgggtcgca ttcttttaca atatatatcc
     6121 ctcaaacact gggtgaatca ccacgccaat tctatcctta tcaagcatac tttggttcac
     6181 acactttaca ggattggttt gtcagcgata aagatgaata tctgtcgcga attggcatcg
     6241 acaagtatat tgaaaaacta gctgtactag gaaaaacaac aaacacgaaa gaacgctcag
     6301 atatttatgc agaatttttt tcaaagcgag gccgggaggc atttttttgc gcccatctta
     6361 atgaaaaaag acaaccatta cgagtaaagt tcaaaataac tgaaattaac cctgaattgg
     6421 cattaaagaa tttacaggaa acacaagaat ttattgatac ccatccggga gataacccat
     6481 cagataaagt agaaaattat agaaatagag cgaaattggc aatgactgaa cacctggaga
     6541 gcctattaga catcaatcct gaatcgagcc cctctttatt attttggaaa aatgtcgata
     6601 agaaagaaca agaaatgacg gacagtagca ggtataaaaa gggaccaagc tagtcaatga
     6661 aacaataaaa atcaattaaa ttataaattg ttctaaaatt gtattaatgg gtgtttgaaa
     6721 ataacgaagg agtttgattt atgatgagtc gcgaaaaagc agctggaact ttttttaaag
     6781 attaccagaa aaaaaatgta atgagattgt tacaagaatc tctcgaaaaa attatcaacg
     6841 aatggctgaa aactgatgat gaaagccata ccaaactgag aagtcttcaa gaactttcag
     6901 agatggatat taatgctacc tcatttgctg aacactcacc acttcctgat tttgttacgc
     6961 gtctctggct ggatccacat aaagcgcttg atgccttgga ccaaaagatt tcaaaaagcg
     7021 aaatcagaaa gctgattaaa gaaacggcaa gagaaacaga gttagttttc actcatcaaa
     7081 aggcaccgcc ctactcctaa tttaaattag gatagtgcgg gtatattaat tggccattca
     7141 tgttggccat attattggcc aaacatgtct tattggataa aaacattcat agaatgataa
     7201 tacgaaaatt gattcacatc caaaagtcct tctgattaat ttgtcatgtt aaattaagca
     7261 ccttaaatct ggcctccaga gattcgttct tattccacta caaattattt taaatacaag
     7321 gggtcaaatg a
//
LOCUS       DBNCWK010000140         7157 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.140, whole genome shotgun sequence.
ACCESSION   DBNCWK010000140 DBNCWK010000000
VERSION     DBNCWK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.140"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..532
                     /locus_tag="AC06IO_002485"
     CDS             <1..532
                     /locus_tag="AC06IO_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIO9875654.1"
                     /translation="PDLGSLRVLDLCAGCGVIGIELSWYLQAIRQIDFIEIQDIYTKY
                     FYQNIANVNRPELQFRWHLLNYDELHKKKWEDKFDLIISNPPYFQPGHGMLSPSKFKN
                     RCRFYLDSSFQSYIQALGNSLANRGKAYFLLRPLKHHGLDLFSDIQKILQETSVIATK
                     ISHIRGTDIILLEKLK"
     gene            770..3085
                     /locus_tag="AC06IO_002486"
     CDS             770..3085
                     /locus_tag="AC06IO_002486"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIO9875655.1"
                     /translation="MKENVSHLPRELSRMVFWSLTCLKKAIAEVYGKETYERIEQIRL
                     SMQDTIGSESFSLGKALHSLQIELSKLEKKQLYQIAHGFSLMMELINTCENAYRIFRI
                     NQRKEQVYSDMPQGIHYVLTAHPTEARTNEFLKLFKEIRDYLIEVLKSPYLMNEPHLD
                     QMLKISLQINISYHEKPSVEDEAQYIYQYALHPLNIKLYHSFLAKGVPIQLRTWVGGD
                     KDGHSGVNEKIMLKSLELSRSFFIAYIQECLEEVLEIIKLSTALGKNKSLADQINQLV
                     KKAYGLKKIQSKDHEKISAFKNELTATQINALKFLNFEPEQFSIISSIFHLYPALVMP
                     IELREDSTIVKESLTSIKKTTITKMLEQVHSITHGTDPKNYIRGFILSMVESAEDIKN
                     GINLVKKVFKSYALPVVPLFENQLALTNADTILNNSITEDIRQKHLKQWEGNYEVMLG
                     YSDSSKENGVLPSRLMIAKSLKNIQSTLENKNLQPIFFHGSGGSIERGGGDIKEQTAS
                     WSKEMMSNYKVTVQGEMVARLFGSSSILKSQIDKFLDIYSQKGKSNDNDYPEELSLFS
                     TRVSQKYKTLIQSDWFWQIVEQASPYHFLKELKIGSRPTKRKSGPDQRKLRAIPWILC
                     WTQTRLLFPTWWGIGSTWSELNGIEKNRLKALYKENALFAAFVKQLGVTLSKVYLPVW
                     EHYLLQLTGSIEYLKPFQSELESTIQFFYQITDEKDFCFHQPWLGESIQLRSTLIHPL
                     NLIQIEAMKRKDLPLLRKTVTGISCGMLTTG"
     gene            3646..4797
                     /locus_tag="AC06IO_002487"
     CDS             3646..4797
                     /locus_tag="AC06IO_002487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875656.1"
                     /translation="MITTEILNIKNELIKRINTCSTQSDSRLDLSSLDLDRLSIDSLI
                     ETMQKIPKSVTTLNLSDNGLYQLSTEHLAAALISIPKSVTTLNLSDNGLYQLSADRLA
                     AALTSIPKSITTLNLSDNYLYYLGADDMAKALKGVPENVTTLNLSWNHLNKLSTDGLV
                     IALTGLPKSITMLDLRCNDLCELGADGLTTTLTRLPKNIAMLDLRCNDLYELGADGLG
                     KILKNIPNNVTELDLSNNGLFILNAVDLAKALAQIPSHVKTVRLGDNGLFINKTYKQI
                     DAFLENLGEQRHRFILSGHGESQLARALGPVAQLTQGNYPIDNSALPVLQRDVAAHIL
                     SFLETNQKPKNPIDFFRSQLKTTIERIECIQSNKRTQTEPDVSKIPSLK"
     gene            complement(4909..6162)
                     /locus_tag="AC06IO_002488"
     CDS             complement(4909..6162)
                     /locus_tag="AC06IO_002488"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIO9875657.1"
                     /translation="MNTDIANQLRAAKKATTDLNLIQSDTRTIILKTLAANLEKHIEN
                     IIQENQKDLSLMLEQDPRYDRLLLNKERILSLANDVRKVASLPNPLGVNLLEKSMPNG
                     LNIKKITVPLGVIAVIYESRPNVTIDIFSLCFKSGNVCILKGGKEAHFTNSYLLLLIK
                     NTLKNFNINTDIVCLLPPERALMTQLLNATGFVDLCIPRGSQNLINFVRDNAKIPVIE
                     TGAGIVHTYFDKSGDLEKGKKIINNAKTRRVSVCNALDTLIIHTDRLKDLPELVKALS
                     KNNVIIYADQEAYQALDKSYPEQLLMKAKPQDFGHEFLDYKLAIKAVPNIQAAIDHVQ
                     QFSSHHSEAIIAEEESAIDQFLTEVDAAAVYANASTAFTDGGEFGLGAEIGISTQKVH
                     ARGPMGLEALTSYKWVIRGTGQIRN"
     gene            complement(6171..>7157)
                     /gene="proB"
                     /locus_tag="AC06IO_002489"
     CDS             complement(6171..>7157)
                     /gene="proB"
                     /locus_tag="AC06IO_002489"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIO9875658.1"
                     /translation="NQIIYLKNLGHQVILVSSGAVGSGRKIAKNSLGKTYGDTVGEKQ
                     LLASIGQPELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLLTELLKHQNII
                     PIVNENDSVAIEELMFTDNDELAGLIAAQINADYLILLTNVDGVFTSHPNDPESKLIN
                     TIDPNSGWPNVSAVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVESIVPKILNRE
                     AVGTIILASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRNGNRVMSILPIGIEDFR
                     GECKRGDLIDILNSNNEKIGVGIARYDFLRLKDYLRLQNKPAFIHYDQLHIL"
BASE COUNT         2274 a         1394 c         1262 g         2227 t
ORIGIN      
        1 tccagatctt ggttcattaa gagtattaga tctctgtgca ggctgtggag taattggaat
       61 tgaactgtca tggtatcttc aagcaatccg gcagattgat tttattgaga tccaggatat
      121 ttatactaaa tatttttatc aaaacatagc taacgttaat cgacctgagc tgcaatttcg
      181 ttggcatctc ttaaattatg atgaactaca taaaaaaaaa tgggaagaca aattcgattt
      241 gattatcagt aatccaccct atttccaacc aggtcacgga atgctttccc cttcaaaatt
      301 taaaaatcgg tgcagatttt atttagatag ctcttttcag agttatattc aagcgctggg
      361 aaattcactt gcaaacagag gtaaagctta ttttttatta cgtccattaa aacaccatgg
      421 ccttgattta ttttctgaca tacaaaaaat cctgcaggaa acatcagtaa tagcaacaaa
      481 aatatcgcat atccgtggta cagatattat tttgttggaa aaactgaaat gaatagtagt
      541 attctttata gaacattgat tttggtctgc cttaaaaaat aaaaatattt ttcaacacaa
      601 attaagtata ttaaaattgc atgccaaagt attcttgaat ggttgtgcca aaatcctttg
      661 agaatcaact agagaagtag acaatgaagt atttactgca tcagagttag ataacgaaca
      721 gacggcattc ctctgcagaa taaaccattc ggataaaatg gagttttcga tgaaagaaaa
      781 tgtgtcccac ctgccaagag agctcagccg tatggttttc tggtcactta cctgtttaaa
      841 aaaggccatt gcagaagtct atggaaaaga aacatatgaa agaatcgagc aaattcgact
      901 atccatgcaa gacacgatcg gaagtgagtc gttttcactt gggaaagcac ttcatagtct
      961 tcaaatcgag ctttccaaac ttgagaaaaa acagttatat caaatagccc atggtttttc
     1021 tcttatgatg gagctgatta atacctgtga aaatgcctac cgcatttttc gtatcaacca
     1081 aagaaaagaa caagtttatt ctgatatgcc tcaaggtatt cattatgtat tgacagcaca
     1141 cccaacagag gctcgtacca atgaattttt aaagcttttc aaagaaattc gtgactacct
     1201 tatagaagtt cttaaatccc cctatctgat gaatgaacct catcttgatc aaatgcttaa
     1261 aatctcactt caaattaata tttcctatca tgaaaaacca agcgtggaag acgaggctca
     1321 atacatctat cagtacgcac tccatccact taacatcaaa ctgtatcatt catttttagc
     1381 aaaaggggtc cctattcaat tacgcacctg ggtaggaggt gataaggatg ggcactctgg
     1441 tgttaatgaa aaaatcatgc ttaaaagcct agagctttct cgttcttttt ttattgccta
     1501 tattcaggaa tgcttagaag aagtgctgga aatcattaag ctttcaactg cattaggtaa
     1561 aaacaaatcc ttggcagatc agattaatca gcttgttaaa aaagcgtatg gtcttaaaaa
     1621 gatacaatca aaagatcatg aaaaaatatc ggcgttcaaa aatgaattga ctgctacaca
     1681 gataaatgcc ctaaaatttt taaatttcga acctgaacaa ttctccatca tctccagtat
     1741 ttttcatctg tatcccgctt tggtcatgcc tatagagctt agagaggact caaccattgt
     1801 aaaagagagt ttaactagca taaaaaaaac aacaattact aaaatgcttg aacaggtaca
     1861 ctctataact catgggactg atcctaaaaa ctatatacgc ggttttatcc ttagtatggt
     1921 tgaatcagct gaggatataa aaaatggaat taatctggtt aaaaaagttt ttaaaagcta
     1981 tgccttgcca gttgtgcctt tgtttgaaaa tcaacttgcc ctcactaacg cagatacaat
     2041 attaaataat tcaatcactg aggatatcag acagaagcat ctcaaacagt gggaaggaaa
     2101 ctatgaagtc atgttgggtt actccgactc gtccaaagaa aatggcgtac tgccctctcg
     2161 gcttatgatt gcaaaaagtt taaaaaacat tcagtctacc ctggaaaata aaaaccttca
     2221 gcccatcttt tttcatggtt cgggcggtag tattgagcga ggaggcgggg atatcaaaga
     2281 acaaactgct tcctggtcga aggaaatgat gtctaattat aaagtaactg tccagggaga
     2341 aatggtagca agactctttg gttccagttc catactcaaa agtcagattg acaaatttct
     2401 tgatatctat tcgcagaaag gcaaaagcaa tgacaatgat tatccggaag agttgtcctt
     2461 gttttcgaca agagttagcc agaaatataa aacacttatc caaagtgact ggttttggca
     2521 aatagtagaa caagcttccc cttatcattt tctgaaagaa ttgaaaattg gctctcgtcc
     2581 aaccaaacga aaatcaggcc ctgatcaacg aaagcttcgc gccatcccat ggattctgtg
     2641 ctggactcaa acacggcttc ttttcccaac ctggtggggt ataggttcca cttggtcaga
     2701 gctaaatggc attgaaaaaa acagacttaa ggctctttat aaagaaaatg ctctttttgc
     2761 tgcttttgta aagcaattag gtgtaacact ctccaaggtt tatctacccg tctgggaaca
     2821 ttacctgctt caattgactg gctctattga atacctaaag ccttttcaat cagaactaga
     2881 atcaactatt cagttcttct atcaaataac tgatgagaaa gatttctgtt ttcaccaacc
     2941 ctggcttgga gaaagtattc aattacgatc aacattgatt catcctttaa atcttattca
     3001 gattgaagcc atgaaaagga aagacttacc tcttttaaga aaaacagtca caggcatttc
     3061 ttgtggtatg ttgacaacag gatgatgggg gtaaacaggg cgattaatga atactctagc
     3121 agaaaattct gtatacgcgg cttttgtcgc agctgaattg aagcaagctg gtcttgcgtg
     3181 gtgtcggctt ttttaacaac ggcaattttg tcggatatat gagctttgtt caacgctccc
     3241 aatgcagcac caagtgggtc attgattatg aatctaataa ttaaagccaa cccccatgca
     3301 caaaagacca aatacaactc tatatgattt acaatgaatc atttgttgat ataatacaat
     3361 actattatta acacaacatg tcgttgtatc atgaaaactc tttggcaaac caatgccttt
     3421 ggtgcttttt atactcaggc tcgcttcccg agtcacaagc tcattcctga cgcttatcgt
     3481 ccctaaacgt ttggttgtac atcaacaacc cattattttt taacccccaa atgcaaacgc
     3541 ttttctgcat ggcttgtcgc ataaggcaat ctctttttgt gcaccagccg ttgagctaag
     3601 tcctgcaggt catgagcgtt aaggcgtagc aataaggtaa acatcatgat tacaacagaa
     3661 atcctaaata taaaaaatga gctgattaag cggattaaca cttgttctac tcaaagcgac
     3721 tcaaggcttg atttatcatc cctggatctc gatcgactaa gtattgatag cctaatagag
     3781 acgatgcaga aaatcccaaa aagcgtcacc acgcttaatt tgagcgacaa tggtctttat
     3841 caactaagca ctgaacacct ggcagcagcg ctgatcagca ttccaaaaag cgtcaccacg
     3901 cttaacttga gcgacaatgg cctttatcaa ctaagcgctg atcgcctggc agcagcgctg
     3961 accagcattc caaaaagcat caccacgctt aatttgagcg acaattacct ttattatctg
     4021 ggcgctgacg acatggcaaa ggcactgaaa ggtgttccag agaacgtcac cacacttaat
     4081 ttaagctgga atcaccttaa taaactaagc actgatggcc tggtaatcgc actaactgga
     4141 cttccaaaga gtatcacaat gcttgatttg agatgtaatg acctttgtga gctaggtgct
     4201 gatggtctga caaccacgct aactagactt ccaaagaata tcgccatgct tgatttgaga
     4261 tgcaatgacc tttatgagct aggtgctgat ggcctgggta aaattctaaa aaatattcca
     4321 aacaatgtca ccgagcttga tttaagcaat aatgggcttt ttattctcaa tgccgttgac
     4381 ctagcaaaag ctctggcgca aatccctagt catgtaaaaa cggttcgtct tggcgataat
     4441 ggtcttttta tcaataaaac ctacaaacaa atagatgcct ttcttgagaa tttgggtgaa
     4501 cagagacatc gctttatttt gtcaggccat ggagagtctc agctggcaag agccttgggc
     4561 ccagttgctc aattgacgca aggcaattac ccaatagaca attctgcatt acccgtatta
     4621 caaagggacg tggccgctca tattctttcc ttcttggaaa ccaatcagaa gcctaaaaat
     4681 ccaattgatt ttttcaggtc tcaactgaaa accacaattg aaaggattga atgcatccag
     4741 agcaataaaa ggactcaaac tgagcctgat gtgtctaaaa ttccgtctct aaaataaaag
     4801 gaggccgtgc agcaccttaa gtcatcagca tgcacggctt ttgtcgaaac taatttagat
     4861 tattgtagct gtcattattg ggaacggctt tgagaaaatt ggttgcactt aatttctaat
     4921 ttgtccagtc cctcttataa cccatttgta gcttgttaaa gcctctagtc ccatggggcc
     4981 tcttgcatga actttttgcg tactaattcc aatttcagcc cctaatccga attccccgcc
     5041 atctgtaaat gcagtagaag cgttggcata gacagctgca gcatccactt cagttagaaa
     5101 ttgatcaatt gcagactcct cctctgcaat aatagcctca ctatgatggg aactgaattg
     5161 ttgtacatga tcaatggctg cttgtatatt gggtactgct tttattgcta atttatagtc
     5221 taaaaattca tgaccaaaat cttgtggttt tgccttcatt aaaagttgtt caggataact
     5281 cttatctaaa gcctgatagg cttcctggtc ggcatatata atcacattat ttttagataa
     5341 tgccttcaca agttctggca agtcctttaa acgatcggta tgtataatca aagtatccaa
     5401 tgcattgcaa acacttactc ttctggtttt ggcgttgtta ataatttttt tacctttttc
     5461 caaatctcca cttttgtcaa aataggtatg tacgatgcct gcccctgttt caatgacagg
     5521 tattttggca ttgtctctga caaaattaat taaattctgg ctccctcttg gaatgcataa
     5581 atcaacaaaa cctgtggcat ttaataattg agtcatcaat gctctttcgg gtggtaataa
     5641 acaaacaata tctgtgttta tattaaaatt ctttaatgta tttttaatta ataaaagcag
     5701 ataactgtta gtaaaatgtg cttcttttcc accttttaaa atacaaacgt ttccagattt
     5761 aaagcatagg ctgaatatat caatagttac attaggtctt gactcataaa tgactgcgat
     5821 gacacctaaa ggtactgtga ttttcttaat attcaaacca tttggcattg atttttcaag
     5881 gagattaact cctaatgggt taggtaaact ggctactttt ctcacatcat tcgccaatga
     5941 caaaattctc tctttattta acaataaacg atcatatctg gggtcttgct caagcattaa
     6001 acttaaatct ttttgatttt cctgtattat gttttcaatg tgcttctcta aattagctgc
     6061 taatgttttc aaaattattg ttcgggtatc actttgtatt aagttaagat cagtagttgc
     6121 ttttttagcg gctcttaatt ggttagcaat atcagtattc attttgatac ctatagaata
     6181 tgcaattgat cgtaatgtat aaatgcaggc ttattttgaa gtctaagata gtcctttaat
     6241 cttaagaaat catacctggc gattccaact cctatctttt cattattgga attaaggata
     6301 tcaattaaat cccctctttt gcattctcca cgaaaatctt ctatccctat tggtaaaatg
     6361 ctcattacac gattaccatt tcgtaataag gtaattaaat tatcattaat tactatagat
     6421 ggtaaggaag ttctatgact aaaagcaatc catcttttta tactggattt tcttttacta
     6481 gcaagaatta tagttccaac tgcttcccta tttagaattt ttggaacaat ggattctaca
     6541 tttacatttg cgatcactgt attgatacct aaaccagcca tttttctggc agtatccagt
     6601 ttggatacca taccacctcg gcctaatttt gattttacag cagagacatt gggccaacct
     6661 gaattggggt caattgtatt aatgagtttt gattcaggat cattgggatg ggatgtaaac
     6721 acaccatcga cattggttaa gagtattaga taatcagcat taatttgagc tgcaataagt
     6781 cctgctaatt catcattatc agtaaacatt aattcttcaa tggccaccga atcattttca
     6841 ttaacaatag ggataatatt ttgatgcttt agtaattccg ttaataaacg ggcaatattt
     6901 aaataatgct gtctagtatg aaagtcttgt ttggtaagta ataactgaga tactaaaatg
     6961 ttttcatttt taaacaagct agaatagaga ttcatcaatt cgggttggcc tattgaagcc
     7021 aagagttgct tctctcccac tgtatcgcca tatgttttcc ccagagagtt cttagctatt
     7081 tttcgaccag agcctacagc cccagagctt actaaaatga cctgatgccc caggttcttt
     7141 aaataaataa tttgatt
//
LOCUS       DBNCWK010000141         7150 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.141, whole genome shotgun sequence.
ACCESSION   DBNCWK010000141 DBNCWK010000000
VERSION     DBNCWK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.141"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(63..1259)
                     /gene="ralF"
                     /locus_tag="AC06IO_002490"
     CDS             complement(63..1259)
                     /gene="ralF"
                     /locus_tag="AC06IO_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214205.1"
                     /note="RalF, a virulence factor secreted by type IV
                     secretion systems (T4SS), is a guanine nucleotide exchange
                     factor that acts on host cell ADP-ribosylation factors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS guanine nucleotide exchange effector RalF"
                     /protein_id="HIO9875659.1"
                     /translation="MHPEIEKAQRDITEAFNAKPKNGIEKIKEICEEYKISPNEEIAD
                     FFHQQRKNLDLEAVGDYLSGPEAENQQVLKAFTSQMDFNGQSFVEGLRTFLKTFKLPG
                     EAQKIDRLVQSFSGAYSQQNPDVVSNADAAYLLAFQTIMLNTDLHNPSIPEKNKMTVD
                     GLKRNLRGGNNGGDFDAKFLEELYSEIKAKPFELNFVKTSPGYELNSTTLNKDSTFKK
                     LNSLLHSTDVNINTVFPRIGDNVKTTVDQPKSWLSFFTGYKGTITLTDKQTSAQATIQ
                     VYTPNIFSKWLFGEQPRVIIQPGQTKESIDLAAKAAAGFSSPVKNFKATYDYEVGDLI
                     KAYDNQKKLISPNKNMVIKGSMFRTPNAEQQETSKSATRTVNTDYDINEDTVTRKDES
                     RSSYKK"
     gene            1494..2828
                     /gene="lem17"
                     /locus_tag="AC06IO_002491"
     CDS             1494..2828
                     /gene="lem17"
                     /locus_tag="AC06IO_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem17"
                     /protein_id="HIO9875660.1"
                     /translation="MEDESKVKKGLKEEKEQLNQKWLEQLLNNPRWGPSVVKLAMARK
                     KENRNQRGEAFNCFVLSRPQNAFEEDEYRKFLDKLKNNINDFSGTTRFQIALFTADNH
                     HWTAFDFFIVDGKLHVFCLDAANDSAGDAALDEIMSRFPDCDAYRLEPDRDPKAKDSK
                     HFRLIQTDRENCSRMTIEHILLLSKKKFLFDENFANYKVVNGVKLINPDALFRQSPDL
                     YRMTQIDSILDSLMIASPKEAQRLVSKKGLNPEQYRDLLGHTSGKQSKSSKNTTIDYK
                     RQRVEKNLLAFLAESEQKHLEDIIDNSEKQWSEFLEMSKSERTQFLLGNVREKPFKEI
                     VGNILGKGIKDRLDHKKYIENDVIKKSSFTLFRGIDKHFEIKKIKAEINELEKALKIV
                     NQLSNDTSADEVLLHLQKQKLPLEIKVELAGALDIYTQLDKEKSALQSHKMN"
     gene            complement(2923..3681)
                     /locus_tag="AC06IO_002492"
     CDS             complement(2923..3681)
                     /locus_tag="AC06IO_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875661.1"
                     /translation="MFFKREKYTPIFFIAVLEKYDNNPAHQNITSSYDEELHTPAVLT
                     KYLSRDIKFLVAILEANKGHLAKDDELYKNITDALRFAEYDLERAKNNLNGIHSKRKK
                     AKNKKDINLESEWNAANDALQKCQTHFDRMESFKNWLDETEEEYLDKDTCKLLKSNIK
                     TLQNLADKITHLTKKANLIYQKYKDFWGENQENIEEKLDSKVQKIDKKEIEKFEKITS
                     SLVKLYHSLPVQHQFLDNFLQTVCLAMPTAKRLA"
     gene            3922..4833
                     /locus_tag="AC06IO_002493"
     CDS             3922..4833
                     /locus_tag="AC06IO_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875662.1"
                     /translation="MDPLETNVTYFVIGKRIKTLESESKLRKKISDEELKEAVPIEEA
                     WLFRTREEADNYAIGTQGYNSKVPECPRNCVAIFEVQLRNPVAKPKTDTIQINPQGSK
                     MIEFQFARVDADNLRYISGGVPGIQGKYQFKPTDEANIKTPKEWLDKIGFDRKIEDLD
                     KKASMLGKEDARASEDLAFIIGNLKKYKREFLDGHKSAEQFVEACRDEIKPEKTKNLA
                     NHRGIFASVTSFIESIVQLVSPKYKINTDSINKINEVEKSLGEIKPERVVKETGIAKR
                     TIKSDYEVDSDNTVAKAGDRRPGIKPG"
     gene            5104..5472
                     /locus_tag="AC06IO_002494"
     CDS             5104..5472
                     /locus_tag="AC06IO_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIO9875663.1"
                     /translation="MLAPEISLLDHLPGYIGWKDLNRHYIGANKALLELKGFRHVEEL
                     AGKTDEELSPWAIEENKLFQQQDFRVINGKKVATVHFNAKTSEVFLLEKYPLTDQNNQ
                     VTGLIYHYHIPARLNVIKSL"
     gene            5577..6560
                     /locus_tag="AC06IO_002495"
     CDS             5577..6560
                     /locus_tag="AC06IO_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIO9875664.1"
                     /translation="MRRLLITILIFLLSNAGCCAPVFEIIPLGVYGGLKDGNLSAYLI
                     KAIESKNYTALDGGTLVHGLEVAADKQSVRNKNVDDLLQKHIPAYLISHAHLDHVMGL
                     VMAQPELREKQTIMAREETIQALQKNIFNWSVWGNFGDSGEIPHLNYQHYQTIPLLEW
                     VAIPDTDLHVKVFSLSHGGMASSAFLIRYKTEYVLYFGDTGADHIEKSTNLENIWKEI
                     APLIKNRQLHAIMLECSFLNSQPDDKLFGHLKPNLFMLELRKLASIVDPSDFRNSLQG
                     LPVVVTHIKPRLADFSNSNEDTEKQVIIELSQENDIGVELIKPEQGIKLSL"
     gene            complement(6700..>7150)
                     /locus_tag="AC06IO_002496"
     CDS             complement(6700..>7150)
                     /locus_tag="AC06IO_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIO9875665.1"
                     /translation="EIITLNGGHAPVEDYHKKFTVKDVALKLMGGKPAYPFARKWFSP
                     KFDADLRHQMNIAAKSLLVDAINNPKIHFVFNRSHGTIEDSLRRNFYNANLIQEISLN
                     ESTQDAGNEHALVRKQFEAHTFFHNQCKKQLTKHGVSIPEFPIKALA"
BASE COUNT         2243 a         1193 c         1364 g         2350 t
ORIGIN      
        1 caagtcgcgg cacgtaggcg ttgggagcca attgtcaaca cgccctaatc ttttataaat
       61 tattatttct tataactgga tctactttca tcttttctag tcactgtatc ctcgtttata
      121 tcatagtcag tgttaaccgt tcttgttgca gattttgaag tttcttgttg ttcagcgttt
      181 ggtgttctga acatactccc cttaattacc atatttttat tgggcgaaat gagttttttc
      241 tggttatcat atgcttttat taaatcacct acctcataat catacgtcgc tttaaaattt
      301 tttactggtg atgaaaaacc agcggctgct tttgctgcca aatcgattga ctctttggtt
      361 tgtccaggtt gtattattac tcttggttgt tctccaaata gccattttga aaatatattg
      421 ggagtataaa cctggatagt agcttgtgca gacgtttgct tatccgttaa cgttattgtt
      481 cccttatatc cggtaaaaaa acttaaccat gattttggtt gatctacagt tgttttaaca
      541 ttatcaccta ttcttggaaa aactgtattg atgtttacat ccgttgaatg caagagtgaa
      601 tttagttttt taaaggtaga atccttattg agagtggttg aattaagttc atatcctggg
      661 ctggttttaa cgaaattcaa ctcaaaaggt ttggctttaa tctcactgta taattcctct
      721 aaaaatttgg catcaaaatc tccaccatta ttacctcctc tgagatttct tttgagtcca
      781 tccacggtca ttttattttt ctcagggata cttgggttat gtaaatcagt attaagcata
      841 atggtttgaa atgctaaaag ataggcagca tccgcattgg aaaccacatc aggattttgc
      901 tgggaatagg caccactaaa gctttgtact aacctgtcaa ttttttgagc ttcgcctggt
      961 aatttaaatg ttttcaaaaa ggtccttaaa ccctcaacaa aactctgccc attgaaatcc
     1021 atttgactgg taaaagcttt taatacttgc tggttttctg cttctggccc actcaaataa
     1081 tcgccaaccg cttctaaatc caaatttttt ctttgttgat gaaagaaatc ggcaatctct
     1141 tcattcggag aaattttata ttcttcacat atttctttta ttttttctat cccatttttg
     1201 ggtttggcgt taaaagcctc agtaatatct ctttgtgcct tttcaatttc tggatgcata
     1261 agctgctcct tttgttgaat gaacagatga attctgctta gataaaaaat taatcggcat
     1321 tccgttttaa tagtgagcgt ttgtgattat attataaccc aattgatcta attttgcctt
     1381 ttatagacaa taaacacttg cggatttaag ttcttgatga ctacaaaaat atcgtagtgt
     1441 gctttatacg ttctatacta gttaatatac aattagattg aagataagcg gctatggaag
     1501 atgaatccaa agtcaaaaaa gggttaaaag aagaaaaaga acaacttaat caaaagtggc
     1561 ttgagcaatt attgaataac ccacgctggg gcccttccgt cgttaagttg gctatggcaa
     1621 gaaaaaaaga aaatcggaat caacgtggtg aggcatttaa ctgttttgtg ctgagtagac
     1681 cacaaaatgc gtttgaggaa gatgaatacc gaaaattcct ggataagtta aaaaataaca
     1741 ttaacgattt tagtggaacg acccgatttc aaatcgctct ttttactgca gataaccacc
     1801 actggacagc atttgatttt tttatcgttg atggaaagct tcatgttttt tgtttagatg
     1861 cggctaatga tagtgcagga gatgctgcgc ttgatgaaat catgagccga tttcctgatt
     1921 gcgatgctta ccgattagag cctgatagag atccaaaggc gaaggattca aaacattttc
     1981 gtttgattca aacagaccga gaaaattgtt cacgaatgac cattgagcat attttgctgt
     2041 tgtcaaaaaa gaaattttta tttgatgaaa atttcgccaa ctacaaagtg gttaatgggg
     2101 tgaagctgat taatcctgat gcactattca ggcaatctcc agatctgtat cgtatgactc
     2161 aaatcgattc tattctggat agccttatga ttgcttcacc aaaagaggcg cagcgactag
     2221 tcagtaagaa aggtttgaat ccagagcagt acagggattt gcttggacat acatcaggta
     2281 aacaatccaa atcatcaaaa aatacaacta tcgattacaa gagacagagg gtggagaaaa
     2341 accttttagc ttttttggcc gagagtgagc agaagcatct ggaagatatt attgataact
     2401 ctgaaaaaca atggtctgag tttttagaaa tgtctaagtc agaacgcacc caatttttat
     2461 tgggcaacgt cagagagaag ccttttaaag aaattgtcgg gaatattcta ggcaaaggca
     2521 taaaagacag gctggatcat aaaaaataca ttgaaaacga tgtgatcaaa aaaagttctt
     2581 ttactctttt tagaggaatt gataagcatt ttgaaatcaa gaaaattaaa gcagaaatta
     2641 atgaactgga aaaagccttg aaaatagtaa atcaattatc taatgacaca agtgctgatg
     2701 aagtattgtt acatttacaa aaacagaaat tacccctgga gataaaagta gaattggcgg
     2761 gggcgctgga tatatatact caattagaca aagaaaaatc tgctctacag tctcataaaa
     2821 tgaattagtt tcagattatc tgtctggtca taacccagcc tgtgtttgat ttgtctgaaa
     2881 tacttttgac ttaatctcca acatggacaa ttaaattgga aattaagcca gccttttggc
     2941 tgttggcata gctaaacaga cggtctgtaa aaagttatcc aaaaattgat gctgtacagg
     3001 taggctatga tatagtttaa ctaagcttga tgttattttt tcaaatttct caatttcttt
     3061 tttatcaatt ttctgaactt tggaatctaa tttttcttct atgttttctt ggttctctcc
     3121 ccaaaaatct ttatattttt gatatatcaa gtttgccttt tttgttaaat gagtgatctt
     3181 atcagccaga ttttggagag tttttatatt tgactttaat agtttacatg tgtctttatc
     3241 caaatactct tcctctgttt cgtcaagcca gtttttgaat gattccattc tgtcaaaatg
     3301 ggtctgacat ttctgtaacg catcatttgc cgcattccat tctgattcaa gatttatatc
     3361 tttcttgttt ttggcttttt ttcgttttga atgtatgcca ttcaaattat ttttagctct
     3421 ttccaggtca tattcagcaa atcgcagagc gtcagtgatg tttttataca attcatcgtc
     3481 ttttgccaag tggcctttat tcgcttctaa aattgctaca agaaatttga tatcccgact
     3541 taggtacttt gttaaaacag ctggtgtatg aagttcttca tcatacgaag aagtgatatt
     3601 ttgatgggca gggttattat cgtatttttc taaaactgct ataaaaaata taggtgtata
     3661 tttttcgcgt ttaaaaaaca tttctagatc ctctggttaa taatactttt aagtgttgtt
     3721 attttaatca ataaatctgt gattttaatt aacagacatg tcgaatttgc gacataatag
     3781 ggaatttttc attcagattt ataaagttaa tgtgccctat ccctacctct tgtggagtac
     3841 agaatttagg gtaaagaact ttgaattgtt tattttttaa actattctta tatgagataa
     3901 gttcactgga ggctatttta tatggacccg ctagaaacga atgttaccta ttttgtgata
     3961 ggcaaacgaa ttaaaaccct agaatctgaa tccaaactaa gaaaaaaaat ttctgatgag
     4021 gaactaaaag aggctgtccc gattgaagaa gcttggctgt ttcgtacacg agaggaagct
     4081 gataactatg cgataggtac gcaaggatat aatagcaaag taccggaatg tcctagaaat
     4141 tgtgtggcta tatttgaggt tcaacttaga aatcctgtcg caaaacctaa aacagataca
     4201 atacaaatta atccacaggg tagtaagatg atagaattcc aatttgcaag ggtagatgct
     4261 gataatttgc gttatatttc aggtggagtt cccgggattc agggtaaata tcaatttaag
     4321 ccaacagatg aggcaaacat taaaactcca aaggaatggt tagataaaat cgggtttgac
     4381 agaaagattg aagatctgga taaaaaagcg tcaatgctcg gaaaagagga tgcccgtgca
     4441 agcgaggacc tggcattcat tattggtaat ttaaaaaaat ataaacggga atttcttgat
     4501 ggtcataaat cagctgagca atttgtagag gcttgtagag atgagataaa acctgaaaag
     4561 acgaaaaact tggcaaatca ccgtgggatt tttgcctcag tcacatcatt tattgaatct
     4621 attgttcaat tagtaagccc aaaatacaaa attaatacag attccattaa taaaataaat
     4681 gaagttgaga aatcattagg tgaaatcaaa ccagaacgag ttgttaagga aacaggtatc
     4741 gcaaaaagaa caattaaatc tgactacgaa gtagatagcg acaatacagt ggctaaagct
     4801 ggagatagga gaccaggtat taaaccaggg tagtttacag agtggcttca tatgaattgg
     4861 gctttcataa attcgacgtg ctgatagcta ttaaaaaaag cctgtggtta agagcccatt
     4921 atttcaactc catcccagag tttagaaatg acaataaaat gcttaccttt ctttaaagac
     4981 aatgattgat tagactcggc tctgtgatat aagcagccga ttcggccaac cttccttgaa
     5041 aattgtaccc caaccgttta ataatccatg ctcgaaatga gcattcattg gaggatagga
     5101 ctaatgttgg ctcctgaaat atcattgctt gatcatctcc caggctatat tggttggaag
     5161 gatttaaacc gccattatat aggcgcaaac aaagcgttgc tggaattgaa agggtttcgt
     5221 catgtagaag agcttgcggg taaaacagat gaagagctgt cgccgtgggc tattgaagag
     5281 aataaactgt tccagcagca agattttcgc gtaataaacg gtaaaaaagt agcaacggtt
     5341 cattttaatg caaaaactag cgaagtgttt ctgttggaaa aatatccttt aacagaccag
     5401 aataatcagg taacaggatt gatttatcat tatcatatcc cagcaagact aaatgttata
     5461 aagtcacttt aatcattatg atgttaatct tccaccgtat aaatacggtg attgaagcaa
     5521 tgttgaataa ggtattattt tacaaactta cttgaatcat ttgaaaggaa atcctcatgc
     5581 gccgtttatt gattaccatt cttatttttt tattatctaa tgcaggatgt tgtgcgcctg
     5641 tctttgaaat tattcctctt ggagtctatg gtggtttgaa agatggaaat ttatcagctt
     5701 atttaataaa agccatcgag agcaaaaatt atacggctct tgatgggggt actttggtgc
     5761 acgggcttga agttgcagcc gataaacagt ctgtgcgtaa taaaaatgtt gatgatttgc
     5821 tgcaaaaaca tataccagcc taccttattt ctcatgccca tcttgatcat gtcatggggc
     5881 ttgtgatggc gcaacccgaa ttgcgagaaa agcaaaccat tatggcacgt gaagaaacaa
     5941 tacaggcatt gcagaaaaat atttttaatt ggtctgtttg ggggaatttt ggagatagcg
     6001 gagaaatccc tcatttgaat tatcaacatt atcaaactat cccattacta gagtgggttg
     6061 ctattcctga tacagatttg cacgttaaag tgttttcttt gagtcatggt ggaatggcat
     6121 caagcgcttt cttaatacgt tataaaaccg aatatgtcct ctattttggt gatactggcg
     6181 ctgatcacat tgaaaaatca acaaacctgg agaatatttg gaaagaaatt gcgccgctta
     6241 taaaaaatag gcagcttcat gccatcatgt tggagtgttc atttttaaat tcacaaccgg
     6301 atgacaaatt atttggtcat ttaaaaccaa acctatttat gttggagttg cgaaaattag
     6361 catcgattgt tgatccttca gattttagaa actcactaca aggcttaccc gttgttgtta
     6421 cgcacatcaa acccaggtta gctgactttt caaattctaa tgaagatacg gaaaaacagg
     6481 ttataatcga attatctcag gaaaatgata ttggcgtgga attaattaaa ccagagcaag
     6541 gtatcaagtt aagtttgtaa tttggcgtaa tttctcgata gctgccaaca gggtcaattc
     6601 aatgcagctc tcctgtatcc actcataagg tccagttcaa gagcgtcctt gcgtgcttga
     6661 actggtattg actcagattc atgtaaatgc tttccggctt taagccaatg cttttatcgg
     6721 aaactccgga atactaacac catgttttgt aagttgcttt ttgcattgat tatggaaaaa
     6781 agtgtgggct tcaaattgtt ttcttacaag cgcgtgttca ttgcccgcat cttgtgttga
     6841 ctcgttcaaa ctgatttctt gaattaagtt tgcattgtag aaatttcttc gtagagaatc
     6901 ttcaatggtt ccatgtgaac ggttaaatac aaagtgaatt ttaggattat tgattgcatc
     6961 aacgagcaag gatttggcag caatattcat ttgatgcctc aaatcagcat caaatttagg
     7021 gctaaaccat tttcttgcaa atggataggc gggtttgcct cccattagtt ttaaggccac
     7081 atctttaaca gtgaattttt tatggtaatc ttcgactggg gcgtgacctc cgtttaatgt
     7141 tataatttct
//
LOCUS       DBNCWK010000142         6942 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.142, whole genome shotgun sequence.
ACCESSION   DBNCWK010000142 DBNCWK010000000
VERSION     DBNCWK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.142"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            281..475
                     /gene="csrA"
                     /locus_tag="AC06IO_002497"
     CDS             281..475
                     /gene="csrA"
                     /locus_tag="AC06IO_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIO9875666.1"
                     /translation="MLILTRRIGETLIIGDDVNITVLGVKGNQVRLGINAPKDVSVHR
                     EEIYLRIQQEKESDDSEQAV"
     gene            complement(601..2583)
                     /locus_tag="AC06IO_002498"
     CDS             complement(601..2583)
                     /locus_tag="AC06IO_002498"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIO9875667.1"
                     /translation="MSNTARINYRKDIDALRGVAVLLVIFDHLHINLVPAGFLGVDVF
                     FVISGFLITQIIFRNTKTQTFSLTKFYLNRMRRILPALMLVLLVSSVMSYFLLLVPDL
                     KNYSTSLLATILSSSNIYFWKFINIGYFSTDATIQQLLHTWSLGVEEQFYLIWPIILI
                     VLTKKFTDKKIILGTILLTIISFFAYFLLRKHPLLAYYSPITRAFELLTGALVALNFD
                     NIPAVSNKYLKNLGSILGLFLIIYPACAFTNNDFPSIKILLPCLGTALLIYTGKNNQG
                     FASKIIENRILVFIGLISYSLYLWHWPLISYLNYLKIPINFTIGFVFILILLLTSTFT
                     YKFVELPFRNKLKLSFLNTMLLFFILPLALAVIFYLFSQYKDNFGFNKISPEKLNQVN
                     TYYGLLKQDYGCIDNKDGFSVLPSEKLCTIGDYHSNKKTVLVVGDSHASAYVGMLHVL
                     LSDQHLQAYVVNQSGTPFILGNISNWRENNPMSRNELLRKLIKNKKYDYVVMGGFWDY
                     YPDLPSLDGKKHPPFEVFKKGLREAVKFIVDNKSIPVIMFDNPPLINLSKTCGLTRVS
                     FLDCSNNLREVKKIQNATRNIILNLKNEFPQLILIDPTKIICDKSKCYTSINGTPLYF
                     DEGANSHLNYDGSTLIGKLYLKKFGNPFQTKNSIYS"
     gene            complement(2746..3741)
                     /locus_tag="AC06IO_002499"
     CDS             complement(2746..3741)
                     /locus_tag="AC06IO_002499"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIO9875668.1"
                     /translation="MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQL
                     NDLGIPIIRIPHQGYQLEKPLILLDSNEITKQLHSKGFSQPFNLHLFTSIDSTNRYLK
                     DLPSSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFNCDISKLSGLSLVIS
                     LATVLTINEFIVSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINS
                     DTKNNPLPDKSWCSFFGITNQNYNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKY
                     DYLVGKKVKVSQALNSISGIALGINSFGQLILEDEFGNKHELSSGDTSLALSNFE"
     gene            complement(3745..4482)
                     /locus_tag="AC06IO_002500"
     CDS             complement(3745..4482)
                     /locus_tag="AC06IO_002500"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIO9875669.1"
                     /translation="MIITGFTPLNTQNCILNESRIDLWQFSLDYELHSAYQLLNADER
                     SRADRFYFNRHKRRFTNARATLRIILARYLNTPPERLEFTYNAHGKPNVINSQKLQFN
                     ISHTGEMAILAVGKTYPIGVDIERYSARPYEGIGKNLFSEQEYQELKKAHQSLKPALF
                     FHIWAQKEAFIKASGLGLSYPTKEFHVPTSFPTKQLVDDPLHNMTWQLRSFMPEVCCS
                     GALCYHPSVREIRHGIVQLQQNISLKF"
     gene            4564..5517
                     /locus_tag="AC06IO_002501"
     CDS             4564..5517
                     /locus_tag="AC06IO_002501"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIO9875670.1"
                     /translation="MTRQFLEFEQPIEELNQKIEALRMVGSDNEVNLSEEIARLEAKC
                     SELTENIFSRLEPWQIAQMARHPLRPQTTDYIERIFTDFQELHGDRSYSSAPAIIGGM
                     ARLNGEPVMVLGHQKGKRTKEKVYRNFGMARPEEYRKALRLMRMAEKFKMPVVTFIDT
                     AGAYPGIGAEERNQSEAIARNLFAMSRIKTPIVCIVTGEAGSGGALAIGVGDKIIMLQ
                     FSIYSVISPEGCASILWKDASKASEAARAMGITADRIFENELVDMVVPEPLGGAHRDV
                     DEMASRLKRLLTSELQALKKVPLDQLIELRYQKFMAMGACD"
     gene            5510..6805
                     /gene="tilS"
                     /locus_tag="AC06IO_002502"
     CDS             5510..6805
                     /gene="tilS"
                     /locus_tag="AC06IO_002502"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIO9875671.1"
                     /translation="MTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLS
                     NQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDF
                     FSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTC
                     PRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHC
                     QQAKANLEVLAIKDCPDLLNATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKT
                     FHRIIHEIIFAKLDAMPTVSWDQIQVRRFQQHLYLLKADQINLPKAIKWQQFPASLTY
                     PDADIKLSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVR
                     DRVPLVYINDQLACVVGYAVSDLFFTTNPLEAWSIVNNS"
BASE COUNT         2199 a         1180 c         1367 g         2196 t
ORIGIN      
        1 tgagtccagt ttatttataa ttgggagttt ttgatattag ggaatgttga acagttttat
       61 gatgaattta tgacaaggaa tttattaatt tttcaactca tttctgcaaa aattaaacgc
      121 aaatttatct atgacttaat attccgttaa taatcagagt gtacaatgtg atccaagcaa
      181 taaattttgc ttaaattttg ttcatattgt agttgccaag aacaccatgg aagcgttcta
      241 tggagaagta ggtagctgct atgttaggga gattttagtc atgttgattt tgactcggcg
      301 tataggtgaa acattaatca taggtgatga tgtcaatatt actgttttag gtgtaaaagg
      361 caatcaagtt cgcttaggaa ttaatgctcc aaaagatgtt tctgttcatc gtgaagaaat
      421 ttatttacgc atacaacaag aaaaagaatc tgatgattcg gaacaagcag tataaatcga
      481 tttccacgca gctcatccag ttccgagtac ttaaaaatac tcgggacgca ctatgtaact
      541 aaatgtaatt tcctctttaa taaagcttct tatacactca tcagcaaatc aaaattaata
      601 ctaactatat attgagtttt ttgtttgaaa tggattacca aattttttga gatatagttt
      661 tccaataagt gtcgaaccat catagttcaa atgagaattt gcaccttcat cgaaatataa
      721 tggtgtcccg ttaattgagg tatagcattt tgatttgtcg catattattt ttgtcggatc
      781 aattaatata agctgtggga actcattctt taagttcaaa ataatatttc tggtagcatt
      841 ttgaattttt tttacctcac gtaagttatt ggagcagtcc agaaaggata cccttgtcaa
      901 tccgcaagtt ttactcaggt ttatcaaggg tggattatca aacataatta ctgggatact
      961 tttgttatca acaataaatt ttactgcttc acgtaatcct ttcttgaata cttcaaaagg
     1021 aggatgtttt ttgccatcca aggatggcaa gtcagggtaa taatcccaaa acccgcccat
     1081 tacgacataa tcatattttt tatttttaat taactttctg agaagctcat tccggctcat
     1141 aggattgttt tctctccaat tcgagatgtt tcctaatata aagggggttc ctgattgatt
     1201 gaccacataa gcttgtaagt gttgatcgga cagcaacaca tgcaacatac caacatatgc
     1261 gcttgcgtga ctgtcaccaa ctaccaaaac agttttttta ttggaatggt aatctcctat
     1321 agtacataat ttttctgatg gtaaaactga aaacccatct ttattatcaa tgcaaccata
     1381 atcttgtttt aacaacccat aataggtatt tacctgatta agtttttccg gagaaatttt
     1441 attaaatcca aaattatctt tgtattgaga aaataagtaa aaaataactg caagagcaag
     1501 aggaagaata aaaaaaagta gcattgtgtt gagaaaggaa agctttaatt tatttctaaa
     1561 cggaagctca acaaatttgt aagtaaaggt tgaagttaat agcaaaatca atataaaaac
     1621 aaatccaatt gtgaaattga ttgggatttt taaataattt aaatatgaaa ttaaaggcca
     1681 atgccacaag tacagcgagt atgaaattaa accaataaag actaatatcc tgttttctat
     1741 aattttgctt gcaaatcctt ggttattttt tcccgtgtaa attaaaagag ctgtacctaa
     1801 acaaggcaaa aggattttaa tgctaggaaa gtcattatta gtgaaggcac aagcagggta
     1861 gataatcaaa aaaaggccta aaatggaacc cagatttttt aaatatttat tgcttactgc
     1921 aggaatatta tcgaaattca atgccactaa tgcgcctgtc aataattcga atgctcgagt
     1981 aattggtgaa taatacgcca aaagaggatg tttccttaac aaaaaatagg cgaaaaacga
     2041 gataattgtt aacaaaattg tgcctaatat aattttttta tcagtaaatt ttttagttag
     2101 tacaattagt atgatgggcc atatcaaata gaattgttcc tctacaccca gagaccaagt
     2161 atgaagtaac tgttgaatgg tagcatctgt tgagaaatat ccaatattaa tgaatttcca
     2221 aaaatagata tttgagcttg agagaatggt tgccagaagt gatgtactgt aattttttag
     2281 atctgggact aataaaagaa aataggacat aacagaggaa actagaagca ctagcattaa
     2341 tgcaggaaga attcttcgca ttcggtttag gtaaaatttg gttaatgaga atgtttgggt
     2401 ttttgtgttt ctaaatatta tctgtgtgat taaaaaacca gatattacaa aaaatacgtc
     2461 aacccctaaa aagccagcgg gtaccaaatt gatatgtaga tgatcaaaaa ttactagtaa
     2521 aacagcaact cctcttaatg catcaatatc ttttctatag tttattctgg cagtatttga
     2581 cattaagttt aaaccctatc aacagatgac gctttattag atttaaaaat gaacattaac
     2641 cttgattttg tacatgttgt gtgaaaagcg attatagaat aaagtgaagt tattaaaata
     2701 ttagatttga aattttactc taaaacttcc tttttgttaa aaaccctatt caaaattgct
     2761 tagggcaagt gatgtatcac ccgaagagag ttcatgctta ttcccaaatt catcttccag
     2821 aatcagttgg ccaaacgaat taatgcccaa ggcaatcccg gaaatcgaat tcaaagcttg
     2881 ggacacttta acttttttcc caacaaggta gtcatactta ttccagtcat ccttaaatgc
     2941 atttagatcg ttattaataa attttgatag atatttttcc aggttaatta atagccttgt
     3001 aacaatcgaa ttccgattgt aattttgatt agttattcca aaaaaagaac accaggattt
     3061 atctggtaga ggattgtttt ttgtatcaga gttgatattt aaaccaacac ctataataaa
     3121 ttgtgctgtc gcgttagatt ctgcaataat ctctactaaa atcccacata actttttatt
     3181 attccacagg atatcatttg gccattttat ttttacttta tcagatacaa tgaattcatt
     3241 tatagtgaga actgttgcta aacttatgac taaactgaga cccgatagct tagatatgtc
     3301 acaattaaaa ctccaccggg tagagcagta aatattctct ccaaaaggag agtgccagtg
     3361 tcgtccaaag cgccctctcc cttgagtttg catttcagtg cagcaaattt cgacggctga
     3421 gcttgaaggc agatctttca ggtagcggtt ggttgagtca atcgatgtaa ataaatgcag
     3481 gttaaacggt tggctaaatc ctttgctgtg taattgcttg gtaatttcat tggagtctag
     3541 caggattaaa ggcttttcca attggtatcc ttgatgtggt atgcggataa taggtatgcc
     3601 taaatcatta agctgattga tttgtttcca tactgctgag cgactgattt ttaaagcgtt
     3661 tcctaattca ctcccactat gacatgcccc atcacccagt atttgcatta acgtatgttg
     3721 agaagtatta aattgaaaca tgatttaaaa ttttaaacta atattttgtt gaagctggac
     3781 gatcccatga cgaatttctc taacagaagg gtgatagcat aaagcgccac tgcagcatac
     3841 ttccggcatg aatgagcgta gttgccaggt catgttatgc aagggatcgt cgaccaattg
     3901 ttttgttggg aaactggtag ggacatgaaa ttctttggtt ggataggaga gacctaaacc
     3961 actagctttg atgaacgctt ccttttgagc ccatatatga aaaaataagg ctggcttaag
     4021 ggactgatgt gcttttttca gttcctggta ttcttgctcg gaaaacaaat ttttaccaat
     4081 cccttcataa ggccgggctg aatatctttc aatatccact ccaatcggat aggttttacc
     4141 cactgccaat atggccattt caccagtatg actgatatta aattgcaatt tttgcgaatt
     4201 aatcacattg ggcttgccat gtgcattgta agtaaattcc aatcgttcag ggggcgtatt
     4261 aaggtatcgg gccaggataa ttcttaaagt tgctctggcg ttggtaaagc gtcttttatg
     4321 gcgattaaaa taaaagcgat cagcacggga gcgttcatcg gcattcaata actgatacgc
     4381 actatgcaat tcatagtcta atgagaactg ccaaagatca atacgtgact catttaaaat
     4441 gcagttttgt gtgtttaatg gagtaaatcc tgtaattatc atctgttgta tctgaaatct
     4501 ggcatcgatt aacggttaag agtatcatat accaaagatg tttaattcac caaagagtaa
     4561 tcaatgaccc gacaattttt ggaatttgaa caacctattg aagagttgaa tcaaaaaatt
     4621 gaagcactgc gtatggttgg cagtgataat gaagttaatt taagtgaaga gatagctcga
     4681 cttgaagcga aatgctccga gttaacagag aatatattct ccaggcttga accatggcaa
     4741 atcgctcaga tggccagaca tcctttaagg ccgcagacta ctgactatat tgagcgaatt
     4801 tttactgatt ttcaggagtt gcatggtgat cgcagttatt cttcagcccc tgcaattatc
     4861 ggaggcatgg cgcgattaaa cggggagcct gtcatggtgc ttggtcacca aaaaggtaaa
     4921 cgtaccaagg aaaaagtata cagaaacttt ggtatggcaa gacctgaaga atacaggaaa
     4981 gcactgcgtt tgatgagaat ggctgaaaaa ttcaaaatgc ctgtagtcac atttattgat
     5041 acggctggag cttatcctgg cataggtgct gaagaacgta accaatcaga agccattgca
     5101 cgcaatcttt ttgctatgtc tcgcattaaa acaccaattg tctgtattgt tacaggagaa
     5161 gccggttcag gtggtgcctt ggcgattggg gtcggtgata agattattat gctgcaattc
     5221 agcatttatt cagtcatttc tcctgagggg tgcgcctcaa tcctttggaa agacgcctct
     5281 aaagcgagtg aagccgcgcg tgctatggga attactgcag acagaatatt cgaaaatgaa
     5341 ttggttgata tggtagtccc tgagcctttg gggggcgcac accgagacgt tgatgagatg
     5401 gcaagtcgct tgaagagatt attaacgagt gaactccagg ctttgaaaaa ggtgcctctg
     5461 gatcaattaa ttgagttaag gtatcaaaag tttatggcaa tgggtgcttg tgactaggcc
     5521 tttactaagt cccgaatggg tagctcgcct taaacaattc aagaaattga tagtagggtt
     5581 cagcggtggt ctggactcta cagtgctcct gcatgtcctt gcctcaactc ctcccttgtc
     5641 taatcaactt ttggctgtcc atattaacca tggcattagt ggaaattctc tgaactggca
     5701 aaggcattgt gagcaattgt gcctggattt aggtattgct tttattgcca aagcgattga
     5761 atttgatcgt tctgcaaatg ttgaagaagc agcacgcaat gcgagatatg attttttttc
     5821 ctccttatta gaggataatg attgtctggt tttggggcat catcttgatg atcaggctga
     5881 aacagttctt ttgcaactgt ttcgcggtgc aggagttgat ggcttggcag ccatgcagga
     5941 atgcagcaat ttgggtgctg ggcaattggc aagaccattt ttgacctgcc ccagagtgga
     6001 attagagcat tatgctcgta ttcatgaatt gaaatggata gaagacgaaa gcaatcaaga
     6061 tacaaaatat tctcgcaatt atctgcgcca tcgcgtaatg ccattattgc ttgaaaagtg
     6121 gccgggagta gcaggtaata tttcccgagc tgcgactcat tgtcagcagg caaaagcgaa
     6181 tcttgaggta ttggctataa aggattgtcc tgatttgtta aacgctactg atcatttgct
     6241 gattgaaccc ataaaaggac ttgaatttga gcggattaca aatgttttaa agttttggct
     6301 taaaaaaaat agaattcaat tgccttcgtc caagacgttt catcgtatca ttcatgaaat
     6361 aatttttgca aagctcgatg ccatgccaac agtgagttgg gatcaaatac aagtacgtcg
     6421 tttccaacaa catctttatt tgcttaaggc agaccagatc aatttaccga aggctattaa
     6481 atggcaacaa tttccagctt ctttaactta tccagacgct gatattaagt tgtctgcagt
     6541 aaaggcccaa aagggattaa tgatcccgaa agatgcgcaa atagaaatac gattcagaaa
     6601 gggtggtgaa gagatttatt ggcatggtca aaccaaacat ctaaaaaaac tatttcagga
     6661 atggcaaatt cccccatggg taagggatag agttccattg gtttacatta atgatcaatt
     6721 ggcttgtgta gtaggttatg cagtgagcga tttgtttttt actactaatc cactggaagc
     6781 ttggtctata gttaataatt cttaatataa caatctccgc tcccttacgg tcgcggctcg
     6841 gttttagcca taatttcatt taatccactc ccttatggcc gcggttcggt tttagccata
     6901 attccattta ataatctgat taatcatctg agccgcgacc gt
//
LOCUS       DBNCWK010000143         6730 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.143, whole genome shotgun sequence.
ACCESSION   DBNCWK010000143 DBNCWK010000000
VERSION     DBNCWK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.143"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(115..1347)
                     /locus_tag="AC06IO_002503"
     CDS             complement(115..1347)
                     /locus_tag="AC06IO_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIO9875672.1"
                     /translation="MNRPVVIVDPLSSGIELAPAFKARGIPAIAVTLKPLDWIGFGAN
                     MQTSDFIEIIPDQPNLVEVLAKYDPIAIIPGTEEGVPLAEALAINLTPQFANDPEKSQ
                     NRLHKAMMQKALQEAGVPALKTLNTASENEVEAWIRTNGLIDSPLIIKPPVSAGSDKV
                     FHIPARGEWKKAFNRVLSEPSKITGKVNATVVVQEQAIGTEFAVGTVSANGKHYLAHL
                     IQYNKTSFNDRKTVYDYVEFVPYSKERYGELFDYTQKALDALGIRWGAAHNEIMLTKD
                     GPRLIETGARMCGGPVVGFAREATGSSQADKLVEIYVDGGVSAEEYVFKKTVVPVFLK
                     SPAKGKISNVEAFADLSKLPTFLNEHIWFKNGDLVPQTVDYLTSIGIIGLAGDRDSIL
                     LDYEKIRNMESKLVIQTF"
     gene            complement(1344..2492)
                     /locus_tag="AC06IO_002504"
     CDS             complement(1344..2492)
                     /locus_tag="AC06IO_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875673.1"
                     /translation="MKKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGV
                     SLFSLTYLFSAPLFSLIFVNQPVKRMIQLALTVFMLGNLITLLSENIVLFLIGRSLAG
                     AGTGIFTPLCISIAIHFASPSAKGRILSFIWSANSAGVVFGVPAGLYLSSLFHWQLSI
                     ASLIILSLLALIGFSMQNIDIKLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGL
                     YSYVTLIQSGSPNSLSMTLLSWGLGGFIGSSLIGVFIDRTGKPRVIMALILVGLMFAL
                     IAIPFTRNLPYLGLIPFFMWGACGWAIVTPQQHILYELHENQGTILAAINSSALGLGS
                     ALGTLLGGLLISSGFRGIYLPFSAATLLFFVLIIQLIVINTSHKVNNI"
     gene            complement(2489..3931)
                     /locus_tag="AC06IO_002505"
     CDS             complement(2489..3931)
                     /locus_tag="AC06IO_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIO9875674.1"
                     /translation="MSAQIADVVIVGAGPVGLMCAYLGQLCGIHTVIVDKSDGPLEVG
                     RADALNARTLQLLELVDLFDELYPLGKTCNTSSVWADGQFISRQSSWWEELEGCLHKH
                     FLMLGQSYVEKLLDDKLKETVAAVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGA
                     DGARSFVRNHFAIPFEIIRPQIIWAVIDGMIETDFPKVPEIIVFQAETSDVAWIPREG
                     EIDRFYVRMDTKDFTLQEAMDKINHAVRPHSLNFKDIVWFSQFSVKESVAERFFIQNR
                     IFLAGDSCHIHSVNGGQGLNTGLADAFNLMWKLHMVMHFGAPKELLHSYEAERKPVAH
                     GVIETSGELVRSTKYSLNGTHAQDYVRIVQKRAGYITGMGIRYGETGLCGSRLFDFEI
                     FNGIAKTRLYSLLDYMKFTLFIFGDREVELQPPEFVKIIPIYPNQCQENFWTNNTHYA
                     NQAILVRPDSYIQYAVPLDKIESLFELAQL"
     gene            complement(3928..4764)
                     /locus_tag="AC06IO_002506"
     CDS             complement(3928..4764)
                     /locus_tag="AC06IO_002506"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIO9875675.1"
                     /translation="MNYKVTSLKPFGVLLEPVSEKTKVTDVDIENLRHLFAKNQLVVL
                     RGFCAFQNAEDFSNYCELWGEVSLWPFGKVLELIEQEDPEDHIFDHSYMPLHWDGMYR
                     PQVPEYQIFHCVKAPLPGQGGRTTFSNTILALQFASSEIKELWNKVSGTYQRKMEFYN
                     SKTVSPIITKHPQKDFSVIRYNEPPSVDKGHFVNPPDIEFTGIDQEELDFFHRSLNKA
                     LYSADNFYAHEWQNGDIVIADNFSLLHGREGFVSRSPRHIQRVHVLSNPPFDNPGLES
                     YQ"
     gene            complement(4754..5803)
                     /locus_tag="AC06IO_002507"
     CDS             complement(4754..5803)
                     /locus_tag="AC06IO_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tyrosine/L-tryptophan isonitrile synthase
                     family protein"
                     /protein_id="HIO9875676.1"
                     /translation="MKNTAFLIKENHCPKDYRLKKSPDSDSAMGVAKRILAEVMIFRR
                     VPETISLCGTGCQKCASPHLPKIISAVNKNEPVTFILPAFPGKSPNPEKVLGHLPDHA
                     ERLSLNFLGTLCQRIKNFYTPGIKIILCSDGRVFSDVVGMNESDVTAYQLELDRLIKE
                     MSLADLSTFNLDYFYKDLHFVQMREELMKNYGQSLDFLKQKIRNGAKPSASPDEQEAN
                     RMYSGITRFLFEDAMHAGQTKSRTAIQKESRFKAYEVIRRSNAWSALIAERFPKAVRL
                     SIHPQTCGSKKLGIRLIGNESWMTPWHGVAVESNKGYVLLRRSEAEALGAKLICSSDG
                     RHSHYQLMAEVSYEL"
     gene            5914..>6730
                     /locus_tag="AC06IO_002508"
     CDS             5914..>6730
                     /locus_tag="AC06IO_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIO9875677.1"
                     /translation="MSLLLDDIKYFIAASDTLNMTRASEIIGISQPALSYSIKRLENK
                     LGGLLFIRLKNGIQLTKLGEEFKRRSHRLLYEWEQVQNIANPESGFVQGNYTIALHPS
                     VALYTLQYFMPKLQVNYPGLGINFIHGHSREMTEKVISWEADFGIVVNPIQHPDLVII
                     KLSTDEVTVFYANDAQNKLIYDRKLAQSQYILKKIGKKITFNGVINSTSLEVVAKLTA
                     LGLGYGILPTRVTYPYKHLKKVLNAPVFRDEICLVYRPEKHNNPVSKKIIQIIR"
BASE COUNT         2065 a         1485 c         1299 g         1881 t
ORIGIN      
        1 aatggataaa ctgtttgttt attaggctaa agaattgttc atataaaact caagtgagca
       61 taatacttca aaccattgaa aaaatatttc aatggcagtt caacaataga aaccctaaaa
      121 tgtttggata accaatttcg actccatatt tcgaattttt tcgtaatcta ataaaatgga
      181 gtcgcggtct ccagccaacc caataattcc aatactcgtt aaataatcaa cggtttgtgg
      241 aacaaggtca ccatttttaa accaaatatg ttcattaaga aaggttggta atttcgatag
      301 gtcggcgaaa gcttcaacat tcgaaatttt gcctttagcg ggtgatttca aaaaaacagg
      361 cacaaccgtt tttttaaata catattcttc tgctgaaaca cccccatcga catagatttc
      421 cactaattta tcggcttgac tactgcctgt cgcctctcta gcaaagccaa ccactggacc
      481 accacacatt ctggcgcctg tttctatgag gcgagggcca tctttagtga gcatgatttc
      541 attatgagca gctccccagc gaattcccaa agcgtccaat gctttttgcg tataatcaaa
      601 taattcacca tatctttctt tgctataagg cacaaattcc acatagtcgt aaactgtttt
      661 gcgatcatta aaggatgttt tattgtattg gattaaatgc gctaaatagt gttttccatt
      721 agcactcact gtgcctacag caaattccgt tcctatggcc tgctcttgca caactacggt
      781 tgcattgact ttcccggtaa ttttagatgg ctccgataaa acccggttga atgctttttt
      841 ccactcccct ctggctggaa tatgaaagac cttatcactg ccagcagata ctgggggttt
      901 aataataagt ggagaatcaa ttaatccgtt tgtcctgatc caggcttcaa cctcattttc
      961 agatgccgta ttgagcgtct taagggcagg aactccagct tcctgcagtg ctttttgcat
     1021 catagccttg tgcaatctat tctgtgattt ttcaggatca ttggcaaatt gaggcgtcaa
     1081 gtttatcgcc aacgcttccg cgagagggac tccttcctca gtaccaggaa taatggcaat
     1141 agggtcatat tttgcaagta cctctacaag atttggttgg tctggaataa tctcgataaa
     1201 atcagaggtt tgcatgtttg ctccaaatcc aatccaatcc agaggtttaa gcgtgactgc
     1261 aatagcaggt atccctcgag ccttaaaggc aggggctaac tctattcctg atgataatgg
     1321 atcgacaatg acaacaggcc tgttcatatg ttatttacct tatgtgaagt gttaattact
     1381 atcagctgaa ttatcaatac gaaaaacaat aaagtggcag cagaaaaagg aagatagatt
     1441 cccctgaatc cagaggaaat caataagccg ccaagcaacg ttcccaaagc tgaccccaag
     1501 cccaaggctg atgaattgat agcggcaagg atagttccct gattttcatg taattcatat
     1561 aaaatgtgtt gctggggagt cactatagcc catccgcaag caccccacat aaaaaaaggg
     1621 attaatccca ggtaaggcag attcctggtg aatggtatgg caatcagagc aaacatgagg
     1681 ccaaccaaaa ttaaggccat aataacccgt ggtttacccg ttcgatcgat aaacacccca
     1741 atcagtgagc ttcctataaa tcctcccagt ccccaactca atagcgtcat actgagcgaa
     1801 ttaggggatc ctgattgaat cagggtgaca tacgagtata gtcccaaact tgccaaggca
     1861 gtaaagcaag taattccaat taccgatagc gtttttggct cgaccagaag acgaagcctg
     1921 cctccaaatg gcgaaggttt gggtagtttt atatcaatgt tttgcattga aaaaccaatc
     1981 aatgcaagca aacttaaaat aataagactg gcaatcgata actgccaatg aaacaaggag
     2041 gataagtaaa gtccggcagg aacgccaaat actacacccg cactgttagc actccagata
     2101 aaacttaaaa ttcgtccttt ggcagatggg ctggcaaaat gtatggcaat gctgatacat
     2161 aacggcgtaa aaatcccggt tcctgctccc gccagagatc ttccgattaa aaacagcacg
     2221 atattttcag aaagtagagt tattaaattg ccaagcatga agacagtaag cgcaagctga
     2281 atcatacgct ttaccggctg attaacaaaa atcagagaaa aaagtggcgc tgaaaaaaga
     2341 taagtcaaac tgaatagact cactccttgc ccagttaccg caaccggctg cccaagcgtt
     2401 tcgcctattt gaggaagcag accagcaatt agaaatgtat tgcaacctac tgtaaacatg
     2461 cctattgcta aaaaaatcag gcttttcttc ataactgtgc caactcaaat aaggattcta
     2521 ttttatccag cggcacagcg tattggatgt aggagtcagg ccttactaaa atggcctggt
     2581 ttgcatagtg tgtgttattg gtccaaaaat tttcctggca ttggtttgga tatattggaa
     2641 tgattttgac aaactccgga ggctgtaact cgacttcacg gtcaccaaag ataaataaag
     2701 tgaatttcat ataatccaaa agagagtaaa gtcgtgtttt ggcaatgcca ttaaaaattt
     2761 caaaatcaaa gagtcgagaa ccacacaaac ctgtttctcc ataacgaatc cccatccccg
     2821 tgatataacc cgcgcgcttt tgcacaatcc tgacatagtc ttgagcatgg gttccattaa
     2881 gcgaatactt ggtcgagcgc acaagctcac cggaagtctc tatcacaccg tgagctactg
     2941 gtttacgctc agcttcataa ctgtgcaaaa gttctttggg agcgccgaaa tgcatgacca
     3001 tgtgcaattt ccacatgaga ttaaacgcat ctgcaagacc agtgttaaga ccttgcccac
     3061 cgtttaccga atgaatatgg caggagtcac cggcaagaaa aatgcgattc tgaatgaaga
     3121 aacgctcagc gacagattct ttaactgaaa attgtgaaaa ccagacaatg tccttaaaat
     3181 ttaaggaatg gggccgcaca gcatgattaa ttttgtccat tgcttcctgc aaggtaaagt
     3241 ctttagtatc cattcttacg taaaacctgt cgatttcccc ttcccttgga atccaggcca
     3301 catccgaagt ttctgcctga aacacgatga tttcagggac ttttggaaag tcggtttcaa
     3361 tcataccatc gatgaccgcc catataattt gtggccgtat gatctcaaaa ggtattgcaa
     3421 agtggttgcg aacaaaggaa cgagccccat cggctccgat gacgtagcgt gactgaatac
     3481 gctctccatt ggcaagagtc gtaagacatc ctgcttcatt aatctcaatg ttgacaatcg
     3541 ctgttgaacg ttttactgct gccacagttt ccttcagctt gtcatctaac aatttttcta
     3601 cataggattg accaagcata aggaaatgtt tatgcaaaca accctccaac tcctcccacc
     3661 atgacgattg tcgggaaatg aattgtccat ctgcccaaac agaactggta ttgcaggtct
     3721 tccctaaagg ataaagttca tcaaataaat caactaattc gagaagttgt aaagtgcgcg
     3781 cgttaagggc atccgctcta ccgacctcta aaggaccatc agacttatcg acaatcacgg
     3841 tatggatacc acaaagttgc cccaaataag cacacatgag ccctactgga cccgcaccga
     3901 caattacaac gtctgcaatt tgtgcgctca ttgataagac tccagacccg ggttatcaaa
     3961 aggcggatta ctcaaaacat gaacgcgttg gatatgacgc ggcgacctgg agacaaaccc
     4021 ttctctgcca tgtaataaag aaaaattatc tgcaatgacg atgtccccat tttgccactc
     4081 atgagcatag aaattatccg ctgaataaag cgctttattt aaacttcggt ggaagaaatc
     4141 caactcttcc tgatcaatac cagtaaattc aatatcgggt ggatttacaa aatgaccttt
     4201 atctacagaa ggaggctcat tgtagcgaat gactgaaaaa tccttttgag gatgcttcgt
     4261 gataattggt gataccgtct tgctgttata aaactccatt ttcctttggt aggtaccact
     4321 caccttattc cataattctt tgatttctga cgaggcaaac tgtaacgcta aaattgtgtt
     4381 tgaaaaagta gtccttcctc cttgtccagg caaaggcgct ttcacacaat gaaaaatctg
     4441 atattcaggc acttggggtc gatacatacc atcccaatgc aagggcatgt aactatgatc
     4501 aaagatatga tcttccggat cctcttgctc aattaactcc agtaccttgc caaatggcca
     4561 aaggctgacc tctccccaca actcgcaata attagaaaaa tcctccgcat tttgaaaggc
     4621 gcaaaagcct cttaatacaa cgagttgatt ctttgcaaat aaatggcgca agttctctat
     4681 atcgacatca gtcaccttcg ttttttcgct cacaggctcc aagagcaccc caaatggttt
     4741 tagagaggtc actttataat tcatatgaca cctccgccat taattgataa tgactgtgcc
     4801 gaccatcaga ggaacaaatt aattttgcac ccaaagcctc agcttccgat cgcctaagca
     4861 agacataccc cttattgctt tccacagcaa caccatgcca aggggtcatc cagctttcat
     4921 tccctatcaa ccgaatccct aatttttttg atccgcaagt ttgtggatga atagacagcc
     4981 gaactgcctt gggaaaacgt tcggcaatga gcgcactcca ggcgttgctt cttcttatga
     5041 cctcataggc tttaaaacga gattcttttt ggatagcagt acgacttttg gtttgacctg
     5101 catgcatggc atcttcaaac aaaaaacgcg taattccgct atacatacga ttggcttctt
     5161 gttcatcggg gcttgctgag ggttttgcac cattacggat cttttgttta aggaagtcca
     5221 gggactgtcc atagtttttc atgagttcct cacgcatttg gacaaaatga agatctttat
     5281 aaaaataatc aagattaaaa gtggacagat ctgcaaggga catttctttt ataagtctgt
     5341 ccagctcaag ctggtaggct gtgacatcac tttcattcat tccaaccaca tcgctaaaaa
     5401 cccttccatc ggaacacaga ataattttaa tcccaggggt ataaaaattt tttattcgct
     5461 gacaaagtgt ccccaaaaaa ttaagagaaa ggcgttcagc atgatctggc aggtgaccca
     5521 gaactttttc agggttggga gattttcccg ggaaggccgg caatataaaa gtaaccggtt
     5581 cattcttatt aactgccgag atgatttttg gcaaatgggg tgaggcgcac ttttggcatc
     5641 cggtgccgca taagctaatg gtttccggga cacggcgaaa aatcattact tcagctaata
     5701 ttcttttggc tactcccata gcagagtcac tatcgggtga ttttttcagt cgataatctt
     5761 tagggcaatg attctcttta attaaaaacg ctgtattctt cattttcctt ccctcctgaa
     5821 aaagagagta cagatcttcg acaaataatt aaaatgattt attttaatta ttgtgataaa
     5881 ttttacttat acctaaagtt aagttgatga gttatgtctt tattgttgga tgatataaag
     5941 tatttcatag cggccagcga tacgctcaat atgacaagag cttctgaaat tattggaata
     6001 tctcaacctg cgcttagtta ttctataaag agattggaga acaaacttgg tggattacta
     6061 tttatcaggc ttaaaaatgg aatccagctt acaaaacttg gagaggagtt taagaggcgc
     6121 tctcaccgtc tattatatga atgggagcag gtacaaaaca tagcaaaccc tgaatcaggt
     6181 tttgttcagg gaaactatac tatagccctt cacccttccg ttgcgcttta tactctccag
     6241 tactttatgc caaaactgca agtgaactat cctgggcttg gcattaattt cattcatggt
     6301 cattcaagag agatgactga aaaagtgata agttgggaag ccgattttgg tattgttgtg
     6361 aatcccatac aacaccctga tctcgttata ataaagctgt ctacggatga agtgacagtc
     6421 ttttatgcca atgatgcgca aaataaactg atttatgata gaaaacttgc ccaatcacaa
     6481 tacatcctta aaaaaattgg caaaaaaata actttcaatg gcgtaattaa ttctaccagt
     6541 cttgaagtgg tagccaaact cacagcctta ggtcttgggt atggcattct acccaccaga
     6601 gtcacctatc cctacaaaca cctcaaaaaa gtactcaatg cgcctgtgtt tagagatgag
     6661 atttgtcttg tctaccggcc tgaaaaacat aataatcctg tcagtaaaaa aatcatccaa
     6721 attattagat
//
LOCUS       DBNCWK010000144         6697 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.144, whole genome shotgun sequence.
ACCESSION   DBNCWK010000144 DBNCWK010000000
VERSION     DBNCWK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.144"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(7..888)
                     /locus_tag="AC06IO_002509"
     CDS             complement(7..888)
                     /locus_tag="AC06IO_002509"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9875678.1"
                     /translation="MLKRVLNTAKAKQPISTTGIFAHTYYWLTSPAGDQSYQNSNYKN
                     EEDNNTGTAIYFIHGTADQPAAFKRVAERLIDAGLPDEICSLNLLAFDQRYQGKSIKF
                     FAEQLKNKIKANQHQRVILMAHSRGGLVASYFAEFLAKEASIEVPLVITVGTPFNGSY
                     LAVKPLSWFSDSIREMEIDSEFLAQLKQEIVEHSVSAYHFFIAKEDAIVPGESGYIKD
                     YVDKYPDSLHILDRHGHLSIMSSHRLVSHIAGLLHDYMDHCENDTKEVKISKLGEFTL
                     IEDYYLEKYEPDSLFNL"
     gene            complement(1127..3183)
                     /locus_tag="AC06IO_002510"
                     /pseudo
     CDS             complement(1127..3183)
                     /locus_tag="AC06IO_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3475..3597)
                     /locus_tag="AC06IO_002511"
     CDS             complement(3475..3597)
                     /locus_tag="AC06IO_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HIO9875679.1"
                     /translation="MTKYRDAGTGRYVTKEFAKKHPGTTVGENSKPKSQRKHKK"
     gene            3742..4437
                     /locus_tag="AC06IO_002512"
     CDS             3742..4437
                     /locus_tag="AC06IO_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875680.1"
                     /translation="MARFFKDREIEIKDSAGEYYKKLCNALKTTMSVCIASTSALVIN
                     ELTAKINKDRMIQYEENSSIAIHGIGEIRVFLLRIYKHLEDNKIDFLDGRAAIKNTLS
                     KGMSGNCEHQAFYLAALLRQQNIPALIYDIEDIGHTVVITQNYLLDPWIGEIFALVKT
                     DLSKFFGSSLNMKASWLNRLLSNKKFSYPDELTLQTLNEYFVQQEDQYRIGLNILDNK
                     KGMDHQMSCQLSY"
     gene            complement(4531..5169)
                     /gene="ravN"
                     /locus_tag="AC06IO_002513"
     CDS             complement(4531..5169)
                     /gene="ravN"
                     /locus_tag="AC06IO_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavN"
                     /protein_id="HIO9875681.1"
                     /translation="MPTYFDPIMQEDTVLDENTIVYLVKIGDNKFSIKAISSGLEHLP
                     SDPTTHAEKYWPIPAKSLIDHSSNKLLFEEDKLTNQPISKDQVIELFAVDPDKTEPKQ
                     FSDSVKRELTENWAREVLQDQFAHAGQNPHFFFYQPLFIHGHHGDNPAQQQDNAMPIP
                     LPLLMFSLIPPMNNNNQQQMTLLFFNILLVPQDHGHEPEQENEGQHAPSNGH"
     gene            complement(5450..5833)
                     /locus_tag="AC06IO_002514"
     CDS             complement(5450..5833)
                     /locus_tag="AC06IO_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875682.1"
                     /translation="MILRLILAFYLIIFPHKLLMANQVMNTAIKVLEECYDKATDLRN
                     YVPCVETEAEKIHTLQNLQIRIKFKNPEKNSKEKVPILMVDKTGYMYYCIATAGKNLT
                     IDSCAGTQGKPLSEGQLMSIELLKD"
     gene            complement(6049..>6697)
                     /locus_tag="AC06IO_002515"
     CDS             complement(6049..>6697)
                     /locus_tag="AC06IO_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875683.1"
                     /translation="FFAPLLSYGAETSNMRGKRYCEIILTKTISSYAVYNTWGLNDCP
                     EQLWNKVSISAVKKETGSSFVHLNGPRYWVIDGFKNTSLINPAIKTISGIPMREAGIL
                     HLSLIDLFKNKPYQSHVVDRKTTWVYQADKPVFELIDPNGQVFVMQSYSVQKYPQTMN
                     TLTQLGAKLQLPKGWKFKTGVLNKPETIQAVNNKAVVVQDNFLNTYQKASHDFLK"
BASE COUNT         2009 a         1097 c         1265 g         2326 t
ORIGIN      
        1 gggcatttat agattgaaaa gggagtctgg ttcatatttt tcaagataat aatcttcaat
       61 tagagtgaat tctcctaatt tggaaatttt tacctctttt gtatcatttt cacagtgatc
      121 catataatcg tggagtaaac cggcaatatg ggataccaaa cgatgagagg acattatgga
      181 aaggtggccg tggcgatcta aaatatgcag cgaatctggg tatttatcaa cgtagtcttt
      241 tatataccct gattctccag gtacaatcgc atcttctttt gcaataaaaa aatggtacgc
      301 actaacggag tgttcaacta tctcttgttt aagttgcgca agaaattcac tatcaatctc
      361 catttctcta atagaatcag aaaaccatga caatggtttt acagcaaggt aagaaccatt
      421 aaacggggtg cccactgtaa ttactaacgg tacctcaatg gaggcttctt ttgctaaaaa
      481 ctctgcaaaa taagaagcaa ctagtcctcc tctggagtgc gccattaaaa tgactcgctg
      541 atgctgatta gcttttattt tatttttgag ttgttcagca aaaaatttga tacttttccc
      601 ctggtaacgc tgatcaaaag ctagtaaatt cagagagcaa atttcatcag gaagaccggc
      661 atcgatcaaa cgttcagcaa cacgcttaaa tgccgctggt tgatcagctg taccatggat
      721 aaaatagatt gccgtccctg tgttgttgtc ttcttcgttt ttgtaattag aattttgata
      781 ggattgatcg ccagcaggag aagttaacca ataataagta tgagcaaata tacccgtggt
      841 ggaaatgggt tgtttggctt ttgcagtatt gagaacgcgc ttcaacatag ttaaattcct
      901 tttttattgt ggagcgtcga gttccgaaag tcggatactg tcaataattt ctcaataata
      961 tagcttaata tgagtcaatt ataaactgtg ctttaaagag ttattggggg cagttattgt
     1021 caatgaatta tgaatgaatt gcgatttggt taacatctct attggttttt tgtaattggt
     1081 gccttactgg tcaaaagcag agagctctta aggtagtaga gtatctttaa agactaaagg
     1141 acgctttgta agagttttca agtggttctg tcaggagatg agaaatagca tgtcgattta
     1201 gatctctgtc tcttctgtat tttaaactaa cttcgggtct ttccagcgat ttactgaggt
     1261 atttttttaa atcagtgcta aatttctcga acaaggattt atcactagtc aacaaacctt
     1321 ttatttggcg ttctaaggct aaagcaaacc atggagtagt atggggaatg attgcttcta
     1381 taattttttg cagtctttct ttgtcagcgc tgctgtcttt tttaatttga gaaatgatta
     1441 ctgcaaaatc agaatgcctt tcgagtgtgg cattagaaat gattatttca aattgttttg
     1501 gggataatgc tttggcagcc attttacaat aaggctcttg agtgtttcta aaaatgtccc
     1561 aaaacttgtt gtcttctatg gaatttttta attgagattc cgataaattt tctagaataa
     1621 ttttcaggcg attttttctg ttctctatga aagatctatg atttattgtc ttggattcaa
     1681 tcatccaaaa gtgttttagc atatcctcat aattaggtaa agaagctctt tctgctatca
     1741 ttttaattct atgatctggc aaactattta aatcttcaat gatctttttc tcattgataa
     1801 tgtacgttgt agcattcagt tcactcagga atgcttcaaa ggcattatca gtccactctt
     1861 cttttgctac agatatattg ggatatgttg gtttggggat agcagttaca gaagaccatt
     1921 ttgagagagg aggtttagtg tggattgtat tggatggcag agcgggtagg gactggtaac
     1981 gttttatacg tgcttgggta ttcatactaa tataatgttc tgccaatttc gcgagttcaa
     2041 tcttgaaaga agcggaatga ggtaaatgga caatctgatc gataagggct attttaattt
     2101 ttgtagtggc ataaggcata acagaagcaa gtttgcctaa caaataagcg cttggtttta
     2161 gtttttttat cattaaattc agaatgtcat gcctgttgat atttgtagca aacaatgcca
     2221 attgtttacg ttttaatgtg ttagctgcag cctcaatgta gtagtcttta tttaaaaggc
     2281 ttaaaaaatc aggcgaatca aaagaagcct ttaactgttc ttctgaaagg gcttcaagca
     2341 tcactgcaaa cttatctttt ctattttctc tttttgaagg ctcaaattca aaaccattaa
     2401 aataacgcca taaataatcc aggtcacttg agccaaattt ccctttttct ttagttggac
     2461 taagtttact gacaaataat tgaatgtctt tatgaaccat ctggttcagc ttattccaaa
     2521 actcatgttt tagtaactca gaatttttca ttatctcaat taatttttca atatgctctt
     2581 cccctgccga agacaggttc tcatcctctt ttatcgggat agccattgcc tcgtcttgtg
     2641 ctatgagaat atttattttt tccataaaag ccataagcac ttcagaattg ttgaaatcat
     2701 gaaattcgac cgctttaaat gattttgttt ttgatttctc ttcagatttt gtgaaaaaag
     2761 cattctgcgt atgagtagta ttgggctgga tagttggctt agtgttggtc gaagtatatg
     2821 gttcttctgt ctctttttct aataatgatt tttttggcgt tgtttcttgt atcttctcag
     2881 tttcgtttgt tgctcttctc actgtaatgg tatcagtcga acttttaagg ccaatttttg
     2941 ttctattaaa tacaaaatca aaaagctctt ttttagaaac aaggccagta agcaaaccaa
     3001 aaattgctct aaaacaaaat atcgaaaagg tgaatataag ccttatgctg ccaacaaagt
     3061 caatagtgat agtgctaaat tgagcttgac gaattattaa atggtggtct tcagattgag
     3121 gaggctggtt tctttttttt gattagagga atgctgctga gattgtttta aaaaaaattt
     3181 cattatatta actaatatcc atatggttaa atgtaatgta agagtaatgg tttattttat
     3241 taaccaaatc atcctgagaa ttcgttaata gagttgaatt cgtgcaaaag atttatattg
     3301 ctggatttat gttaaaacat caatgtctaa atgcaatata cacgtcctgt tttatggaaa
     3361 ttcttcacct tgttatgatt taaaatataa agaaactcac tctggcctat ttttaggaca
     3421 acatgattag tctcttgtgt caaacacaac caaaggccaa ttactgcata gcgattattt
     3481 tttatgtttt ctttgtgatt ttggtttgct attttcacca acggttgttc cgggatgttt
     3541 ctttgcaaac tctttggtga cgtatcggcc ggttcctgca tctctatatt ttgtcatttt
     3601 aaactccatt tttatccgtt ccaatagaag tatagcaaat tcagtttgca agaggcgttt
     3661 tggtgaaaaa gaggctgttc tacttgctcc tctcatactt gtatgtaaac taaaaccctt
     3721 attggatata agggattaga catggccaga tttttcaaag acagggaaat tgaaatcaaa
     3781 gattcagcag gtgagtatta caaaaaactg tgtaatgctc tgaaaacaac tatgtcagtg
     3841 tgcatcgcca gcacttctgc attagttatt aatgaactca ctgcaaaaat caataaagat
     3901 agaatgattc aatatgagga aaatagctct atcgctattc atggtatcgg tgagataaga
     3961 gtttttctac tcaggatata caaacatctt gaagacaaca agattgattt tctggatggg
     4021 agggcggcaa taaaaaacac actgtcaaag ggaatgtcag gaaattgtga gcaccaggct
     4081 ttttatcttg ctgccttgct gagacaacaa aatattcccg cgcttattta tgatattgaa
     4141 gacattggcc atactgtagt tatcactcaa aattatctcc ttgatccatg gataggtgag
     4201 atttttgcgc ttgttaaaac cgatctcagc aaatttttcg ggtcgtcact taatatgaaa
     4261 gcctcgtggc taaatcgatt attaagtaat aaaaaattca gctaccctga tgagcttact
     4321 ctccaaacat taaacgaata ttttgtacaa caagaagacc aatatcgaat cggacttaat
     4381 attttggata ataaaaaggg tatggatcat caaatgtctt gccaactatc ttattaaata
     4441 agcattgccg cctgagcgta actgtcaaaa tagaaatgtc gggcattgtg cccgacttaa
     4501 ccttgttgga aatgcaagtt aggattaatg tcagtgccca ttagaaggag catgctggcc
     4561 ttcattttcc tgctcaggtt catggccgtg atcctgtggt accaggagta tgttaaaaaa
     4621 taataatgtc atttgttgtt ggttattatt attcatgggt ggtatcaagg aaaacatcag
     4681 gagtgggaga ggaattggca ttgcattatc ctgttgttga gcagggttat ctccatgatg
     4741 cccatggatg aacaggggtt gataaaaaaa gaaatgagga ttttgccctg catgagcaaa
     4801 ttgatcctgc agtacctctc gtgcccagtt ttcagttaac tctctcttaa ctgagtcact
     4861 aaattgcttg ggttcagtct tatcgggatc aactgcaaaa agttctatta cctgatcttt
     4921 gcttatgggt tgattcgtta acttatcctc ttcaaaaagg agcttgttac ttgaatgatc
     4981 aatcaatgat ttggcaggaa taggccaata tttctccgcg tgagtagttg gatcactggg
     5041 gaggtgctcc aatcctgatg aaattgcttt aatactgaat ttattatcac caatcttaac
     5101 taagtaaacg atcgtgtttt catccagcac agtatcttct tgcataatag gatcaaaata
     5161 agttggcata ataatagtcc tttagctgtt atttaaactc aatagtaact tataggatca
     5221 aattggcttt tgcaatactt ttgattcatt atttgatcat gtgaaaacaa taattgcttg
     5281 aaaccaggag aaaaagttat gtgcaaaatg aatgtttttg aaagactatt aattattctt
     5341 tgagtatcga aaataagtat taatcgagtt ttctgggagc ctgttaatta aggcaagatt
     5401 ttacagttta acaatgtcaa tcgcattaag cgcatttgaa taacagatat taatctttta
     5461 gcaactctat tgacatcaat tgtccttcag acaaaggttt tccttgtgtc ccagcgcaag
     5521 aatctatggt taaatttttt cctgctgttg caatacaata atacatgtat cctgttttat
     5581 ctaccatcaa tatgggtact ttttctttag agtttttttc aggattttta aattttatgc
     5641 gtatttgcaa attttgtaag gtgtgtattt tctctgcttc tgtttcaaca catggaacat
     5701 agtttctaag atcagtggct ttgtcataac attcctccaa taccttaata gcagtattca
     5761 taacttgatt agccattaaa agtttatggg ggaaaataat caaataaaaa gcaaggatta
     5821 atcttaatat cattataatt aacctctaag atactccgta taggttaatt ataatacaac
     5881 atttgtaaaa tgtttgttat tttaccttgg tttgctaatc ataggtttta tagatgcttg
     5941 aaattgccat taacacgttc attaatgcca ttagccccaa agtagaggat ggattgtcaa
     6001 cgcatactaa actcatgacc tatgaaagta accaattatc agctgaattt atttcaggaa
     6061 atcatggctg gctttttgat aggtgttcaa aaaattatcc tggacaacta cggctttatt
     6121 attaacagct tgaattgttt ctggcttgtt aagcacacca gttttaaatt tccaaccttt
     6181 gggaagctga agtttggcgc ctagttgtgt gagggtattc attgtttgtg ggtatttttg
     6241 aacactatag gattgcatta caaaaacttg tccgttggga tcaatcaatt caaagacagg
     6301 tttatctgct tgatagaccc atgttgtttt gcggtccacg acatgtgatt gatagggttt
     6361 atttttaaat aaatcgatta aactaaggtg caaaatgcct gcttcacgca ttggaattcc
     6421 actaattgtt ttgatggctg gatttatcaa agaagtgttt ttgaatccgt caattaccca
     6481 atatcgaggg ccattgagat gaacaaaaga agagcccgtc tcctttttta ctgcggaaat
     6541 agaaacttta ttccataatt gttccgggca atcatttaat ccccaagtat tataaacggc
     6601 ataactggat atggtttttg tcaagatgat ttcgcaatat cgcttgccac gcatgttgga
     6661 tgtttctgca ccataagata gcaagggagc aaagaat
//
LOCUS       DBNCWK010000145         6629 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.145, whole genome shotgun sequence.
ACCESSION   DBNCWK010000145 DBNCWK010000000
VERSION     DBNCWK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.145"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..742
                     /locus_tag="AC06IO_002516"
     CDS             <1..742
                     /locus_tag="AC06IO_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875684.1"
                     /translation="SKIKYLPIAVVSSALNLISLLFYLIGYSLWFIASHFYPSQDKKM
                     QEWYEFAQFKEQYLYAAALGLVATSISMMAIFSPIMLVLSGWLFFGSNIVWTIGEYNK
                     LNNPPSHEENFSKDRQNAYVSYALSISLIGFITAVSTTVAFFIPVMTIPLLIVTTIVC
                     IGVGALAFEYWIESKFGDAQPDITIDESYKQMSNDLGKKITLEPNPTPEPYHGTRPLH
                     FTPHDVKMREPSPDLQMDLSSRTCKPQY"
     gene            781..1176
                     /gene="cdd"
                     /locus_tag="AC06IO_002517"
     CDS             781..1176
                     /gene="cdd"
                     /locus_tag="AC06IO_002517"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIO9875685.1"
                     /translation="MDKLTSNMINEAHKVLVNAYAPYSKFSVAACICTDKNNFYTGVN
                     VENISYGLTTCAEVSAICNMVTAGERKIKSIVVLAANNLLCSPCGACRQRIHEFSSSD
                     TMVHLCNKETIFHSITINELLPLAFDFNP"
     gene            1189..2028
                     /locus_tag="AC06IO_002518"
     CDS             1189..2028
                     /locus_tag="AC06IO_002518"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIO9875686.1"
                     /translation="MTTSISTISYPHLAAEYIQKTCPSFKPKVGVVLGSGLGQFAEEL
                     EDTVAIEYEKLPGFPRTTVQGHGGKLILGYYGSTAVICLQGRAHTYESMENHEAVKTY
                     VRTLKLLGCQYFIATNASGSLKEEVGPGELMLITDHINFQPGNPLVGPNDDEFGPRFY
                     PLDNAYDITMRNALLDIAQKHSIKLHQGVYISVLGPNYETAAEIRAFKLLGADAVGMS
                     TVPEVLVANHCGLRVAVIATITNYATGLAKTSHSHESVVKMASSAAEKLNTLIKQYIL
                     ELS"
     gene            2025..2789
                     /gene="deoC"
                     /locus_tag="AC06IO_002519"
     CDS             2025..2789
                     /gene="deoC"
                     /locus_tag="AC06IO_002519"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIO9875687.1"
                     /translation="MSLDKHFNDVLNKLLSNQDIISISNAQLMHCIDLTLLEADASLE
                     ALHQLQSQACQNNVAAICVLPKHLHVFKPNSKIKLATVINFPLGNDDLLVSLAEIDNA
                     IQLGANEIDYVLPYQYYLRGNKQKALNHCDVIIQTCKKHQLTLKIILETGLFPDMESI
                     YQLSSEIIALGGRFLKTSTGKTPQGATLAAVFAIVSAVMDSTYTSSCGIKVSGGIKTP
                     LQAQQYAQLAELLMGKAIHKDWFRIGASTLLEKLLS"
     gene            2842..4614
                     /locus_tag="AC06IO_002520"
     CDS             2842..4614
                     /locus_tag="AC06IO_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9875688.1"
                     /translation="MKLWIMLFNCFIVTFLITSSHAKAPVNKNKSSYCLPNQSCWPTP
                     KEWEQLNKKINGHLIKARNYLSPCIEDPKSNGCKNTLEEIKNPFYLETQPTATQSTGW
                     FEAWDSEISPYIVAAKTAQEIAHAVKFARKHHIKLVVKGTGHDYLGRSNAPDSLLVWT
                     HHMREIQFQKHFVPNGCSANHKGIPAVTAEAGTRWIEAYREVTENQGRYVQGGGCTSV
                     GVAGGFIQGGGFGSFSKKYGTGAGGILEAEVITADGSILIANECQNQDLYWALKGGGG
                     GTYGIVSRVTLETHELPATFGALTGSITAKTEQDFKNLIDYFIIFYRERLHNEHWGEQ
                     VILGPDNKMELALVFQGMSKTQVDRLWQPFKTWLSQKPEHYQFTINSLVLPARKFWDY
                     DYLSKNLPDHIVIDKRKNIPYPEFWWTGNQSEVSIYLTHYQSGYLPYTLFQEKFSNKL
                     SNALFEASRLTKLSLHFNKGLAGASQQAIERQRKTSMNPSVLDAAALITIIGGQQYVF
                     PKLPEHKPDLDKARFSAKQAKKAMKLISALNPDSGTYGNEADYNLKNWQRALWGSNYE
                     KLLQIKHHYDPENLFNCHHCVGSE"
     gene            4694..5092
                     /locus_tag="AC06IO_002521"
     CDS             4694..5092
                     /locus_tag="AC06IO_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875689.1"
                     /translation="MKTKIIFKSISSLLLGLLLVSCSNELPPGEYDANEIGKIKKVIP
                     GVIISKRPVNLHRQNADANLHVKGGADSNLIDGGYNRTHGVEYVIKLNSGTIISVVQA
                     EDLKLKNKQKILVIYGRHTRVVADNGSDVY"
     gene            complement(5226..6074)
                     /gene="ubiA"
                     /locus_tag="AC06IO_002522"
     CDS             complement(5226..6074)
                     /gene="ubiA"
                     /locus_tag="AC06IO_002522"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIO9875690.1"
                     /translation="MIPWNAYVRLLRLNKPIGILLLWYPTAWALWMANQGFPSIDLLM
                     IFLLGTVFMRSAGCVINDIADRHIDRHVARTQFRPLTSGEVSLSEAFILLFVLLCASL
                     FLLLKLPINCFYFAVISVLITFLYPFCKRFLNAPQLVLGLAFSMGIPMAFIASGKNLN
                     SDFIVLFLINFSWIIAYDTMYAMTDKADDLKIGVKSTAIYFASYDRLIIALLLIFLHS
                     LWLVWAINKNVEWFFYLLWCTAAGILTYQLKLIYARIPKNCFKAFLVSGYYGLVMWFA
                     VGLALI"
     gene            complement(6071..6607)
                     /locus_tag="AC06IO_002523"
     CDS             complement(6071..6607)
                     /locus_tag="AC06IO_002523"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase family protein"
                     /protein_id="HIO9875691.1"
                     /translation="MSINYQSLLIADTKGPGLLKDWLEYQGSLTDKLKAMTGNAELER
                     LSQNWSIPNWWDKYVLCIQDQSILQREIVMKNQGIVYWYARSVIPQSCYALKPEFFNR
                     LENESIRNLIFDESSVRRLPILCYPVDQLNLEFHWVKKYIGSEYSQMWVRFTELVFQE
                     KSSFYLVEILLPELESLL"
BASE COUNT         2214 a         1239 c         1229 g         1947 t
ORIGIN      
        1 ttcaaaaatt aaataccttc ccattgccgt ggtttcttcc gctctgaatc tgatttcact
       61 tttattttac ctgattggct atagcttgtg gtttattgca agtcattttt atccaagcca
      121 agataaaaaa atgcaagagt ggtatgagtt tgctcaattt aaagaacaat atctttacgc
      181 cgcagctcta gggctcgtcg caacatctat cagtatgatg gcaatctttt ccccaataat
      241 gcttgttctt tcaggatggc tgttttttgg cagtaatatt gtttggacaa ttggtgaata
      301 caacaaatta aataatccac ccagccatga agaaaatttt tccaaagacc gtcaaaatgc
      361 ctatgtgtct tatgctttat ccataagttt aataggattc attacggcag tctcaactac
      421 tgtcgctttc tttattccag taatgactat acccttactt atagttacaa ctattgtatg
      481 tattggagta ggagcgttag catttgaata ttggatcgaa tcgaaatttg gcgacgctca
      541 gcctgatatc actatcgatg aaagctacaa acaaatgagc aatgatttgg ggaaaaaaat
      601 tactttggaa cccaatccta ccccagaacc ttatcatgga accaggccac ttcactttac
      661 gccacatgat gtaaaaatga gagaaccgtc tccagacctt caaatggatt tatcctctcg
      721 tacttgtaaa ccacagtatt aatttagtat attctgcacg ccaatgatga gggtgtgaca
      781 atggataaat taacttcgaa catgattaac gaagcgcata aggtactagt caacgcttat
      841 gccccctatt ccaaattttc agtagctgcc tgtatatgca ctgataaaaa taatttttat
      901 acaggggtga atgtagaaaa tatttcttat ggtctaacaa cctgcgctga agtttcagcg
      961 atttgtaata tggttaccgc aggtgagcga aaaattaaaa gcattgtggt attagctgca
     1021 aataatttgc tatgttctcc ttgtggcgca tgccgacaaa gaattcatga gttttcatcc
     1081 tctgatacca tggtacatct ctgtaataaa gaaacaatat ttcatagtat taccattaat
     1141 gaattactac ccttggcttt tgattttaac ccttaatcgg acgattttat gacgacttca
     1201 atatccacta tcagttaccc acatttagca gcagaatata ttcaaaaaac atgtccatca
     1261 tttaagccaa aagtgggtgt agtattaggc tcagggctag gtcaatttgc cgaagagtta
     1321 gaagatacag ttgctattga atacgaaaaa ctcccaggat ttcccagaac tacagtacag
     1381 gggcatggag gaaaattaat tttaggatat tatggatcta ctgcggtcat ttgcttacaa
     1441 ggcagagccc atacttacga aagcatggaa aatcatgaag cagtaaaaac ctatgtaaga
     1501 actttaaagc tattaggttg ccagtatttt atagcaacaa atgcttctgg ttcacttaaa
     1561 gaagaagttg gaccggggga attaatgcta attaccgatc acatcaattt tcaacccgga
     1621 aatcccctgg ttggtccaaa tgatgatgag tttggaccca ggttttatcc tttagataat
     1681 gcttatgaca ttaccatgcg caatgcatta ttggatattg ctcaaaaaca ctcgattaaa
     1741 ctacaccagg gggtttatat ctctgtcttg ggaccaaact atgaaacagc tgctgaaatt
     1801 cgtgccttta aattgctggg agctgatgca gtggggatgt caactgtgcc tgaagtttta
     1861 gtcgccaatc attgtggctt gcgtgtcgct gtcattgcaa ccataacaaa ttatgcaact
     1921 ggccttgcaa aaaccagtca tagccatgaa tccgttgtca aaatggcttc cagtgctgct
     1981 gaaaaactta atactttgat taaacagtat attttggaat tatcgtgagt ttggataagc
     2041 attttaatga tgtcttgaac aagctcctgt caaatcagga tattatatct atttcgaatg
     2101 ctcagttgat gcattgcatt gatttgacac tactagaggc ggatgcttct cttgaagcat
     2161 tgcatcaact acagagccaa gcctgtcaaa ataatgttgc agctatttgc gtactaccca
     2221 aacatcttca tgtatttaag cctaactcca aaattaaatt agcaactgtt atcaattttc
     2281 ctcttgggaa tgatgatttg ctggtttcct tggcagagat agataatgcc attcaactgg
     2341 gtgctaatga aatagattat gtactaccct atcagtatta cttaagagga aataagcaaa
     2401 aagcgttaaa tcattgtgat gttattattc aaacctgcaa gaaacatcaa cttaccttaa
     2461 aaataatttt agaaactggc ctttttccag atatggaaag catttatcag cttagctcag
     2521 aaatcattgc tcttggtggg cggtttctca aaacttcgac tggaaaaacc cctcagggtg
     2581 caactctggc agcagtattt gctatagtca gtgctgttat ggattcaact tatacatcct
     2641 cttgtggaat aaaggtttca ggagggatta aaacgcctct tcaggctcaa caatatgctc
     2701 aactggctga attacttatg ggcaaagcga ttcataagga ttggtttcgt attggagcca
     2761 gtactctgct tgagaaatta ttgtcatgaa cgcacaatct atgtgttaat atgtcagcat
     2821 gcaaacggga gaggtcttgc aatgaaatta tggataatgc tatttaactg ctttattgtt
     2881 acctttttaa taacatcctc tcacgcaaaa gccccagtaa ataagaataa atcatcttac
     2941 tgcctcccta atcaatcatg ctggccaacc cccaaagaat gggagcaact aaacaaaaaa
     3001 ataaatggcc atttaataaa agccaggaac tatctgtcac cttgcattga agatcccaaa
     3061 agtaatggtt gtaaaaatac cctggaggaa ataaaaaatc ccttttattt ggaaacacaa
     3121 cctacagcaa cccagtccac cggctggttt gaggcatggg attcagaaat aagcccatac
     3181 atagttgcag caaaaacagc tcaggaaatt gctcatgcag taaaatttgc cagaaaacat
     3241 catatcaaac tagttgtcaa aggcactggt catgactatc ttggaagatc caatgctcct
     3301 gattctttgc tggtttggac acatcacatg cgagaaatac agttccagaa acatttcgtc
     3361 ccaaatggtt gttctgcaaa tcataagggt attcctgcgg taactgctga agcaggtacc
     3421 agatggatag aggcttatag agaggttact gaaaaccagg ggcgctatgt tcagggcgga
     3481 ggctgtactt ccgttggagt tgctgggggg ttcattcaag gtggaggatt tgggagtttt
     3541 tctaaaaaat atggaaccgg tgcaggggga attttagaag cagaagtaat taccgctgat
     3601 ggttcaattt tgattgccaa tgaatgccaa aaccaggatt tatattgggc attgaaggga
     3661 ggtggtggcg gaacttatgg tattgtaagt agagtcacat tggaaacgca tgaattgcca
     3721 gcaacatttg gagcattaac aggaagtatt acagcaaaaa cagagcagga ttttaagaat
     3781 ctcatagatt attttattat tttttacagg gaaagacttc ataatgaaca ttggggagaa
     3841 caagtcatat taggccctga taacaaaatg gagctggcat tggtttttca agggatgagc
     3901 aaaactcagg ttgatagatt atggcaacca tttaaaactt ggttatccca aaaacctgag
     3961 cattatcaat ttacaataaa cagcctggta ctgcctgcaa gaaaattctg ggattacgat
     4021 tatctaagca aaaatctacc tgatcatatt gtaattgata agcgaaaaaa catcccttat
     4081 ccagagtttt ggtggactgg taatcagtca gaagtttcta tctatttaac tcactaccaa
     4141 tccggttatt tgccctatac cctctttcaa gaaaaatttt cgaataaact atctaacgct
     4201 ttgtttgaag catctcgcct gaccaaactg tctttgcatt ttaacaaagg cttggcaggg
     4261 gcgtctcagc aagcaatcga gagacaaaga aaaacatcaa tgaacccttc cgtattggat
     4321 gcagcagcct tgataacaat aattgggggg caacaatatg tctttcccaa gttgcctgag
     4381 cacaaacctg atttagataa agcacggttt tcagctaaac aggcaaaaaa agcaatgaag
     4441 ctaatatctg ctctgaatcc tgattcgggc acttatggaa atgaggccga ttacaattta
     4501 aaaaattggc aaagggctct atggggatcc aattatgaga aattattaca aattaaacac
     4561 cattatgatc ccgaaaactt gtttaattgc caccattgtg ttggaagtga ataaaaatta
     4621 attaataatt ttaagaatct cagttagaat aactagtcat agtgaattaa tcagcattgg
     4681 agtttaaatt atcatgaaga ctaaaatcat atttaaatcc atttcatcat tactattggg
     4741 tttgctactt gtaagttgta gcaatgaact tcctcccggc gaatatgatg caaatgagat
     4801 aggcaaaata aaaaaggtta ttccgggtgt gattatttcc aaacgaccag ttaatttaca
     4861 tcgtcaaaac gctgatgcca atctgcatgt aaaaggcggt gctgattcta atttgataga
     4921 tggtggttac aatagaacac atggtgtcga atacgtcatc aagttaaact ctggcaccat
     4981 tatttcagtg gtacaagcag aagacttgaa attaaagaac aagcaaaaaa ttctggtaat
     5041 ttatggaaga cacactcgag ttgtagctga taatggaagc gatgtatatt aattagcctt
     5101 ccagtttcaa aatataggtt ggtgttggat tctttctttt aaagcctgtc ttatgaagga
     5161 attttccgac acccataatc tcttgacata acaaattgtc tattagggtt gagaggacat
     5221 attatttaaa tcaatgctaa tccaacggca aaccacatta ctagtccata ataaccactc
     5281 actaaaaatg ctttaaaaca atttttaggt attcttgcat aaattaattt tagttgataa
     5341 gtcagtattc cagcagcggt acaccataac aaataaaaaa accattctac gttcttattt
     5401 attgcccata ccaaccataa agaatgcaga aaaatcagta acaaagcaat aattaatcta
     5461 tcataacttg caaaatatat tgcagtcgat ttgactccta tttttaaatc atcggctttg
     5521 tcagtcatcg catacatcgt gtcataagcg ataatccagg aaaaattaat taaaaacaat
     5581 acaataaaat cgctatttaa atttttccct gaggcaataa atgccatagg aatacccatg
     5641 gaaaaagcca aacctaatac cagttgtgga gcattcaaaa atcgtttaca aaatgggtat
     5701 aaaaaagtaa ttagcacgga aataaccgca aaataaaaac aattgattgg aagttttaat
     5761 aataaaaaca atgaggcaca aagtaataca aacaataaaa taaatgcctc agacaagctg
     5821 acctctccag aagtcaaggg gcgaaattgg gttcgggcta catgcctatc aatatgcctg
     5881 tcagcaatat cgttaataac acaacctgcg gaacgcataa atactgtacc taataaaaaa
     5941 atcataagca aatcaataga aggaaagccc tgatttgcca tccaaagtgc ccatgcggtg
     6001 ggataccaaa gtaataaaat tcctattggt ttatttaagc gtaataatct gacataggca
     6061 ttccagggaa tcataataaa ctttccaact ccggtaacaa gatttcaacc aaataaaaag
     6121 aactcttttc ctgaaatact agctccgtaa acctgaccca catctgagag tattcagagc
     6181 caatgtattt tttaacccaa tgaaactcaa gatttaattg atctacagga taacaaagta
     6241 ttggtaaccg tctcacacta ctttcatcaa aaatgagatt tcgtattgat tcattttcca
     6301 aacggttaaa aaattctggc ttaagggcat aacacgattg tggaataact gatcgcgcat
     6361 accaatagac aatcccttga tttttcataa caatttcacg ttgaagaatg ctttggtcct
     6421 gtatacacag aacatactta tcccaccagt ttgggataga ccagttctga gataaacgtt
     6481 ccagctccgc attaccggtc attgctttca gtttatctgt taaggaacct tggtattcta
     6541 accaatcttt gagtaatcct ggccctttgg tatcggcaat cagcagagat tgataattaa
     6601 tagacataca taaataattt ggaaaaaaa
//
LOCUS       DBNCWK010000146         6475 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.146, whole genome shotgun sequence.
ACCESSION   DBNCWK010000146 DBNCWK010000000
VERSION     DBNCWK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.146"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            69..596
                     /locus_tag="AC06IO_002524"
     CDS             69..596
                     /locus_tag="AC06IO_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HIO9875692.1"
                     /translation="MNRIKLGISNLIFMGRWLQLPLYLGLILILAGYVYRFIIELFEL
                     IFHLKGLDDTHIMLGVLDLIDVVMIANLLIMVVMGGYETFVSHLQLDSHPDQPEWLDH
                     FDAGAMKIKLALSLIGISSIHLLRTFIDPAKLSNYSIMWQVVIHLTLIVSALAIALTN
                     RLMDQPARKNLQHPD"
     gene            833..2050
                     /gene="legC2"
                     /locus_tag="AC06IO_002525"
     CDS             833..2050
                     /gene="legC2"
                     /locus_tag="AC06IO_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC2/YlfB"
                     /protein_id="HIO9875693.1"
                     /translation="MTDTPKANLAVDEIKEDTPKDQPLPEENTEILVKKLKVLETTQE
                     NFDQIKKHIAAVIDSLAKNRSFFGRVAAYWGEMQLWLKITLGIVLVVPTLTLGIAIQV
                     ASLIVVSVLTLVTYVGSALLLENHVQKEEHITDRLKEEMIGLADNLGKVIESFEPLRK
                     QIAEQIEYFHKENDRLNLNVTQLSDHIMRLTKQAEQLQKTEQTLRKVREELEKTSETL
                     DEKVQVQTELLQINQEELNRIREEIAKNEIELSEKISELDQVKKEMSVEIEKAETVTQ
                     VLKNTVEKLSQTAIADHNHRESFQNKLNDFLSNKEQSFNDVAQRICEAEHELCLVKEE
                     LNFSNKRYQELLERQEEVVKRLEQLQFSRKPTPYSNAKVLGKLGIMATEDKESPTPVS
                     SEENTIPTQTLTG"
     gene            2370..2684
                     /locus_tag="AC06IO_002526"
     CDS             2370..2684
                     /locus_tag="AC06IO_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875694.1"
                     /translation="MSTTKWNLSISRAVICVIALFASMICAAAHFVGGIHTNAGYVGW
                     YHPDYYNHDVIGVGNYHPGYGWVAPVYATPTYIIGNTGYDCQTVQQCDSEGNCIQSQN
                     CD"
     gene            complement(2849..3958)
                     /locus_tag="AC06IO_002527"
     CDS             complement(2849..3958)
                     /locus_tag="AC06IO_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875695.1"
                     /translation="MELKRQCINGVAGVSLSLLAVLIHPLFAEDAPQFVKGQSQLPAA
                     QDPAESGWLPDIIGLPNEEQVESLTRHVAEVMTEEKDTNCNAIDYNLRMLVFQNFVIY
                     QHMANAQHHYFKEKMVLKYAHVLAMILKESSGDPTNVTDMDGRSISTSKPGTNLQRWS
                     NLLKLTMQNGIKFNYQTNFGLTQLSADRLFIAFKLANGASHSKDFLEGKNGDETPNRV
                     DMDSAIAIRRLIWFYQDIAQGRLKQKDECINEEDIYKPEFNERYQAGLRMALLYCGTK
                     FLFRVDNEPVWINENSTFEKAMASIAYCKLGNSKTGYGTDEMDEQCFAEWVTLCPALN
                     VNVALLTPLSYFQTRKNKPVCTDTFNRLLNKEPKN"
     gene            complement(4040..4315)
                     /locus_tag="AC06IO_002528"
     CDS             complement(4040..4315)
                     /locus_tag="AC06IO_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIO9875696.1"
                     /translation="MTKELCMRCYISGRVQGVWFRASAKNLAEQLMISGWARNLADGR
                     VEVFACGKEDKLEEFYTWLQKGPLNARVDVCTRENLPWEDYITFDVL"
     gene            4471..4824
                     /locus_tag="AC06IO_002529"
     CDS             4471..4824
                     /locus_tag="AC06IO_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875697.1"
                     /translation="MKKVILYALPSLMLAILFSPAFADETPAQLTGTPAEQIQQLNAQ
                     IQSQLQEMQTKQQQQLDALNTQLQNQIKQAQSQLQEQIQTVNSQTQDQIKQVQATLQE
                     QIKQVQQQALQKANQ"
     gene            complement(5005..6336)
                     /locus_tag="AC06IO_002530"
     CDS             complement(5005..6336)
                     /locus_tag="AC06IO_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1888 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HIO9875698.1"
                     /translation="MLQFFEKGLQYSGEKISTDSYLMRKIVEAAIPSADKGTIAAEKM
                     LGLVNPNQIEIVSGSESAYRLIVHKIKEAKKQILIQAFVWEPGTQVVKDIRNALCDIK
                     HEVEVFLLVDQLDRLAQIFYHGEILPKKPKHDPACLGLDNLPQNIKLHIGTHVHNSIA
                     SNHNKAIWIDGDVILTGANFQQENYGPNGFHDAAMFIPSGAAEAVFYDFKAMWDKRTN
                     KSDAPEISPIYTGLESTESYRNTNSCSVLYVTNTIRQKWALLPFYRAPLPEDPLNNAY
                     MAAIKYAKHTIQIAVPNLNSPEIIHALADFINNRNGHVYLLMGKGFNDFRERFYGGTN
                     QSAIDYFSSLLDEDRQNNLQVRWFHRVNNNENSMKKEVIHMKFMSIDGQVVIYGSSNL
                     DLLSLHNSHETNIVIDNADFAQRAIEQLYLPIFETSIPVLISPKRTCCVCS"
BASE COUNT         2146 a         1238 c         1091 g         2000 t
ORIGIN      
        1 aacaaaacct tcaggaaagc ttatcataag accattcaat aagttatatt ttgtagtgaa
       61 tcgacaaaat gaatcgaatc aaacttggca tcagcaatct tatttttatg ggaagatggc
      121 ttcaacttcc cttgtatctc ggtttgatct tgatactggc cggatatgtg tatcgcttca
      181 tcattgaatt atttgaactc atttttcatc ttaaaggcct ggatgatacc catatcatgc
      241 ttggagtatt ggatctcatt gacgtcgtca tgattgccaa tcttttgatt atggtagtta
      301 tgggcggcta tgaaaccttt gtatcccatt tgcaattgga ttcccaccct gatcaacctg
      361 aatggcttga ccattttgat gcaggagcta tgaaaatcaa attggctctt tcactgatag
      421 gtatctcatc gattcatctg ttaagaactt ttatagaccc tgcaaaactt agcaattatt
      481 ctattatgtg gcaggttgtg attcatttaa ctttaattgt ttcagccttg gccattgcat
      541 taaccaatcg gttgatggat cagcccgcca ggaaaaacct tcaacaccct gattgattta
      601 aaaatctgaa aattaaataa cctctttctc tgatgtcact cactctgcac atgacaaaac
      661 ctgttatgtg cagagtcccg ctctaaaaaa ataatgactg gcttaaaatt attcgataga
      721 atcagggaag tttgacctat tcagggaact catgggattg aaccagaaag aagtagattg
      781 caagctcttt cgggttgaat gtagtcaaaa tggaaataaa ggaatatgaa tcatgacaga
      841 cactccaaaa gctaatctag ccgttgatga aattaaagag gataccccca aagaccagcc
      901 cctccctgaa gaaaacacgg aaatattggt aaaaaaactg aaagtattgg aaactaccca
      961 agaaaatttt gatcaaatca aaaaacatat tgctgcggtt atagattcct tggcaaaaaa
     1021 tcgcagtttt tttggccgtg ttgccgccta ctggggagaa atgcagttat ggttaaaaat
     1081 tactcttgga atagtattgg ttgttcccac cttgacactt ggaattgcaa ttcaagtggc
     1141 ctctcttatt gtagttagcg tcctgactct ggtgacttat gttggtagtg ccctgcttct
     1201 tgaaaatcat gttcaaaaag aagagcatat tactgatcgt ttaaaagaag aaatgatagg
     1261 tttagcagat aacctgggaa aagttatcga gtcttttgag cctctccgca agcaaattgc
     1321 agaacaaatt gagtattttc ataaagaaaa tgatagatta aacctcaacg ttactcaact
     1381 aagtgatcat attatgcgat taacaaaaca agctgaacaa ttacaaaaaa ccgaacaaac
     1441 tttgcgtaaa gtcagagaag aattggaaaa aacatctgaa actctggatg aaaaagtaca
     1501 agttcaaacc gaattactac agataaatca ggaagaatta aacagaatca gggaagaaat
     1561 tgcaaaaaat gaaattgaat tatctgaaaa aatttctgag ctggatcaag ttaagaaaga
     1621 aatgtcagta gaaattgaaa aagccgaaac tgttacccaa gtattaaaaa atactgtaga
     1681 aaaactgtct caaactgcta ttgcagatca caatcatagg gaatcgtttc aaaataaatt
     1741 gaacgatttt ctctctaata aagaacaaag tttcaatgat gttgctcaac gtatatgtga
     1801 agccgagcac gaactatgcc ttgttaaaga agaattaaac tttagtaaca aacgttatca
     1861 ggaactattg gagcgtcaag aagaagtagt taaacgtttg gaacaactcc aattttcccg
     1921 taagcctacg ccatatagta atgcaaaagt acttggtaaa ttaggtatta tggctactga
     1981 ggataaagag tctccaacac ctgtttcatc agaagaaaat acaatcccaa ctcaaactct
     2041 cacaggttag atgtttttaa atcgatgtgc tatagcacat cgattgctta tcacgtgcgg
     2101 tcatccaaaa accaatcagt gcaacgaaat ttattttaaa acagatcgcc aattacatac
     2161 tgagcataag gctcaaacta aagctattcg aagacagtat aaatgttcta ttaatgagct
     2221 aatttagatc tattgcccat aatcaattta tattaaatca gctaattttt ttactttatg
     2281 aacataaacc cacagagaac aataaaagaa ctatactttt agaatattga ctgtgaccag
     2341 tgaaatgatt ctttgaaaag gagagagcta tgagcacaac caaatggaat ttatccattt
     2401 ctcgcgcagt tatctgcgtc attgcattat ttgcttctat gatttgtgct gcagctcatt
     2461 tcgttggtgg aattcataca aatgcgggtt atgtgggatg gtatcatcca gattattaca
     2521 atcacgatgt tataggcgtg ggtaattatc acccaggtta tggctgggtt gctccagtgt
     2581 atgctacgcc aacatatata ataggcaata cggggtatga ctgccagaca gtccagcaat
     2641 gtgattcaga gggaaattgc attcaaagcc aaaactgtga ttagtagtgg aaaatgtagc
     2701 atatcaatgc gcattatctt ttttacaaga ttgaaaacaa attccactcc acttaggttc
     2761 tctaatcatc aaaactgtta attagattaa tgggtaaaca gcggtaacga taggatttta
     2821 aatgttaaaa gtgataagac aaaggctatc aatttttggg ttctttattc agtaaacgat
     2881 taaatgtatc ggtacaaact ggtttgtttt ttctggtttg aaaataacta agcggagtaa
     2941 gcagtgctac attcacattc aaagctgggc ataaggttac ccattctgca aagcactgct
     3001 catccatttc atcggtacca taccctgttt ttgaattgcc cagtttgcaa taggcaatag
     3061 aagccattgc cttttcaaag gtcgaatttt catttatcca aacaggttca ttatcaacgc
     3121 gaaacaaaaa ttttgtacca caatataaaa gagccattct taggccagct tggtatcttt
     3181 cattaaattc aggtttatag atatcctcct cattaataca ttcatctttt tgctttaatc
     3241 gcccttgagc aatatcctga taaaaccaaa tcaatctgcg aatggcaata gccgaatcca
     3301 tgtctactct gttaggagtt tcatctccat tcttcccttc cagaaaatct ttgctatggc
     3361 tcgctccatt agctaattta aaagcaataa acaacctgtc tgcagacagc tgagtcagcc
     3421 caaaattggt ttgataatta aatttaattc cattttgcat tgttagtttt aataaattgc
     3481 tccagcgctg caaattagtg ccaggtttgc ttgtagatat tgagcgacca tccatatccg
     3541 taacattagt tgggtcacca ctactttctt ttagaatcat ggcaagaacg tgagcatatt
     3601 ttagtaccat tttttctttg aaataatggt gttgtgcatt cgccatatgc tgataaatta
     3661 caaaattttg aaaaacaagc atcctgagat tgtagtctat tgcattacaa ttcgtgtctt
     3721 tctcttctgt catcacctca gcaacatgtc tggtaaggct ttcaacttgc tcttcattgg
     3781 gcaagccaat aatatcagga agccaaccac tttctgccgg atcttgtgca gctggtaact
     3841 gggattgacc tttaacaaac tgtggggcat cttcagcaaa gagtggatgg attaacacag
     3901 ccaataaaga taaagaaaca cctgctaccc cgttaataca ctgtcttttt aattccataa
     3961 gctgtacctt ctttattgag tatagcttgt atctattcga aggcatggca agtaaaaatt
     4021 aaattacaat aggctttttt tataagacat caaaagtgat ataatcttcc caaggtaaat
     4081 tctctcgtgt acatacatca actcttgcat taagaggtcc tttttgcagc caggtataaa
     4141 attcttccag cttatcttct tttccacatg caaaaacctc aacacgacca tccgctaaat
     4201 tccgagccca tccacttatc atcaattgtt cagccaaatt tttagcagaa gctctaaacc
     4261 atactccttg aactcttcct gaaatataac aacgcataca taactccttg gtcataattc
     4321 ttttccacct atcgtaaaaa tatcagttac ttcatttcat ttgaccttgc ataacctgtt
     4381 ttcactctga ttctgctata gttaaattag cattcttatt agagcaaaat aaattagata
     4441 atttggactt ttcaataatg gagataaaaa atgaaaaaag tgatattgta tgcacttcca
     4501 tctttaatgt tggcaatctt gttttcccca gcctttgctg atgaaacccc tgctcaactg
     4561 accggcacac cagctgaaca gattcagcaa ttaaatgctc agattcaatc tcaattacag
     4621 gaaatgcaaa caaaacaaca gcaacaactg gatgctttaa atacccaatt acaaaatcaa
     4681 attaaacaag cccaatctca attacaagaa caaattcaaa cagtcaattc tcaaacacaa
     4741 gatcaaatta aacaagtaca agcaacccta caggagcaga ttaaacaagt acagcagcaa
     4801 gcattgcaaa aggctaacca atagttattt gaatttatat aatttctccc atgtgaactt
     4861 gggtattggc aatgcttatg catctaaaac ttaaagacat tgatggtaat ttgcatgagt
     4921 ttggataaaa atggaattat tttttatgtc gaactcatgt atcatgatta taattaatat
     4981 tggcttgatc aaaacagcct aaaatcaaga acaaacacaa caggttcttt taggcgatat
     5041 caatacaggt atactcgttt caaaaatggg tagatataat tgttctattg ctctttgtgc
     5101 aaaatcagca ttatcaatta caatatttgt ttcatggctg ttatgtaagc ttaacaaatc
     5161 aagattcgat gaaccgtaaa taactacctg cccatcaatt gacataaatt tcatatgaat
     5221 aacctctttt ttcatagagt tttcattgtt attcacacga tgaaaccaac gaacttgcaa
     5281 gttattttgc ctgtcctcat caagtagtga actaaaataa tctatcgcac tctgatttgt
     5341 accgccatag aatctctcgc gaaaatcatt gaatcccttt cccatcaata gatagacatg
     5401 gccatttcta ttattaataa aatccgccag ggcatggatg atttccggcg aatttaaatt
     5461 gggaacagcg atttggatag tatgtttggc atatttaata gcagccatgt aggcgttatt
     5521 caatggatct tcaggtaaag gcgccctata aaaagggaga agcgcccatt tctgccggat
     5581 agtatttgtc acatagagca cagaacaaga atttgtattt ctataagatt ctgttgactc
     5641 caggcctgta tatattggag aaatttctgg tgcatcagat ttattggtgc gcttatccca
     5701 catcgcttta aaatcgtaaa aaaccgcctc cgctgcgccg ctaggaataa acatggcggc
     5761 atcatgaaac ccattaggac cataattttc ctgctgaaaa ttagcccctg tcaaaatgac
     5821 atccccatca atccagatgg ctttattatg gttggacgca atcgaattat gaacatgagt
     5881 acctatatgc aatttaatat tctgaggtaa attatctaaa cctaaacagg ctggatcatg
     5941 ttttggtttt ttaggaagta tttcgccatg ataaaaaatt tgtgccaaac gatccaactg
     6001 atcgaccaga agaaatactt cgacttcatg tttgatgtca caaagcgcat ttcggatatc
     6061 tttgacaacc tgagttcctg gttcccatac aaaagcctga ataagtattt gctttttagc
     6121 ctctttgatt ttatgaacta ttaaacgata tgcgctctca ctacccgata ctatttctat
     6181 ctgatttgga ttcaccaggc ccagcatttt ttctgcggca atagttccct tatcagcaga
     6241 gggaattgct gcttcaacta tttttctcat taaataagaa tcggtggaaa ttttttctcc
     6301 tgaatattgc aaacctttct caaaaaattg gagcatgatt cactcataaa ttaattttac
     6361 ctaatttata tcattgaatt gatttaatat ttttgcaaat taatttgagc gattcaaaac
     6421 aaaatttaat tcatcgtaaa cccaatctca gacaccatcc acctgttatt attat
//
LOCUS       DBNCWK010000147         6443 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.147, whole genome shotgun sequence.
ACCESSION   DBNCWK010000147 DBNCWK010000000
VERSION     DBNCWK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.147"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            301..591
                     /locus_tag="AC06IO_002531"
     CDS             301..591
                     /locus_tag="AC06IO_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9875699.1"
                     /translation="MDKKLFERLYESMTQMNEIIDGERVPSREFVVDAVHVKEIRKKT
                     GLTQEKFCHLIDVSIGTLRNWEQGRREPTGPAKALLRAINKDPEHVLAALAG"
     gene            complement(880..1071)
                     /locus_tag="AC06IO_002532"
     CDS             complement(880..1071)
                     /locus_tag="AC06IO_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875700.1"
                     /translation="MRNKDEKAKEVATDSRCAEANRLTQLITGAILADNCFFPSGVYS
                     IKPMVSSPQPPQEKTTPSM"
     gene            1339..2235
                     /locus_tag="AC06IO_002533"
     CDS             1339..2235
                     /locus_tag="AC06IO_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875701.1"
                     /translation="MKDKTIPSPDAKSFNKEEFSENVAKGAIVGATSTTMTFGLFDKP
                     LAIATKASSEVAQHPWRGIKNLFAGALNGSFSTSWNNSLYAQAYRAYLEHPVKGYPVA
                     LLNSCLKNVVFFPAKYGSERALYALSGNEQQAKNYSGFLAGLMTVYLTAPISVVKARL
                     MTNVPLNTLSLKRLGSGVNAIAMRDCIQYGIYFNAKERLNEKIGDGIMAGGIAGIVGY
                     VFSNPLSVIGLNQKTAVESVNIPNMAKRIYQANGVKGFYPLVGLAAWGMFARGVAIHQ
                     GTKLYESLQENEAYGRPTRNRE"
     gene            2468..3910
                     /locus_tag="AC06IO_002534"
     CDS             2468..3910
                     /locus_tag="AC06IO_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIO9875702.1"
                     /translation="MKISTNELIAVVSQFNPWWRGESIQDLPTWHRAAYQELELWLIK
                     PPVPRAIFLSGARQIGKTTLLLQAIDKLLKEGVKPGNILYVTFDHPLIKMSDIDAVLE
                     AWREREPKTGDLEYIFIDEAQFMRSWGTWIKHQVDFSKSRRIFFTGSATPIIEADQES
                     GVGRWHTIRLTTLSFYEYLQIKKIDLPQLPTVKTLRELFEWPQQEFYKVKELAVHYVG
                     HFHEYLLRGGFPQTAQIENITQAQRLLREDIIDKVLKRDMTALFGVRRILELEQTFLY
                     LCMHDGGLLDMTDLCSNLEVKRPTIQNYIFLLEATNLIYRLPPFGYGKDILRAKYKIY
                     LADAAIAPAVLLKDKSILDDPESLGIATETAVFKHLFARYYSQNVRFSYWRGTKDHEV
                     DVVAQIGDQIIPFEVKYRSQHTNAKALKGLLELCKKKSIAHGYVVTKSLDDFGIMKAL
                     PEIKTNVMQIPAPLLCYWMGESELRQTFGV"
     gene            complement(4072..5559)
                     /locus_tag="AC06IO_002535"
     CDS             complement(4072..5559)
                     /locus_tag="AC06IO_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2912 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875703.1"
                     /translation="MSYSKIDVLERKWSSLEAIVKSYTDPVEYHLPEIKKLMTAEFVV
                     DFSSHAIYLHTEYVHRFTQQLINLQNASPNDERLLYIHQLWTDLLNCRNQNPLKIKHH
                     KKLDGECTFNFSLKMMEGKPYGFKEVQILDPNNTAELNKAMGEIKRIDSLAHGANFGD
                     LPFIGDIVLSSMLHYKETICFIAKDKNDEVIGYCWGMMLRDIPAGEKDKVNVFWVMNL
                     AKHPDFYDPNIKVGEALRQHLANTLSKNPDCHFLGYEHVLNHNFHLAIVDGGVPKKEE
                     TISIGQNQYQAKSLVKYEPLKMQYDRKHIIKTNNHQYPYPKVDVIKSNFFTALWYAAP
                     TAFDFILGGILIYGRAYYQQWTHSMLGQPVTHRIEKPISQEQQICDMNILKRIILSDV
                     WGQQGTTLFFDACIPNTIQKLQQLVKEDQIEFASLQQCVAKSGWALCRSKLTAYFYFA
                     ITQSQYPSAAINLLIQNDMTPKKWIELISEERQIFAQQSSERASI"
BASE COUNT         2024 a         1111 c         1302 g         2006 t
ORIGIN      
        1 aattccaatt tttacagaag acgtgaagga attattatct gatgaggagt atgctgagtt
       61 tcaagagtat cttgctgata atcctttagc tggagacgta attcagcaaa ccggcggact
      121 gcgaaaagtt cgatgatcat ttcaaggcaa agcgaagcga ggcggtgtta gagttattta
      181 ttattatgca attcctgctt cacaaataag actcttactt atttataaaa aaggtattca
      241 agatgatctg actacagatc aaaagaaaat gttacggcaa ttaaatgaac gatggtaatc
      301 atggataaaa aattatttga acgattatac gaaagtatga ctcagatgaa cgaaattatt
      361 gatggtgagc gtgttccttc acgtgagttc gtagtggatg ctgttcatgt aaaagaaatt
      421 agaaaaaaaa caggcctaac ccaagaaaaa ttttgtcatt taattgatgt aagtattgga
      481 acattaagaa attgggagca ggggagaaga gagccaacag gtcctgcaaa agctcttctt
      541 cgcgcaataa ataaagatcc tgaacatgta ttagcagcat tagctggttg ataattgatg
      601 ttttaacttt ctataaaaag tgcagacagt gtcggcacaa ttcaaagttt ctctaaagga
      661 gcttttaact caaaatatag aaaacgagct tagcaagctt atatgaccga taaaatttgc
      721 cagggtcagt aaatagtatt ctgatggcta gataataaat gatgaaaagt tcttttgatg
      781 aaatgaataa aagaaccttt tttattgagt gactcttaaa aaagccattt gctttatcct
      841 gtgcttttgg caagcagtat ggtcgcattc aggctttgtt tacatgctag gtgtagtttt
      901 ttcctgaggc ggttgtgggc ttgataccat aggttttatt gaatagacgc cacttgggaa
      961 aaaacaattg tcggcaagga ttgcccctgt aatcagctgg gtaagccgat tggcttcggc
     1021 acatctcgaa tcagtggcaa cctctttcgc tttttcatct ttatttcgca taacaatcct
     1081 gtgaagttaa tctttgcaga ggacactatg atggtttcat gtcatgcaaa cgaagcaacc
     1141 gggtaccgag ttgagtccaa agctcgatct tgattctttc gctaacgttt gcaacaagga
     1201 gtatttcatt tgtagtccgt tgcgttggtg caaagcaagt taagttaaaa ataatcgacc
     1261 agttaatgtc tggttaagga tagcattata tcattacaga tcaatcgcgc acgtcgtatg
     1321 atttaagagt aataagtaat gaaagacaaa acaataccca gccctgatgc aaaatcattt
     1381 aataaggaag agttctctga aaacgtggca aaaggagcca ttgttggcgc aaccagcacg
     1441 accatgacat ttggtttatt cgacaagccc ttggccattg ctacgaaagc ctcctccgaa
     1501 gtggctcagc atccatggcg tggtatcaaa aatttgtttg ccggggcact gaacggaagt
     1561 ttttcgacca gctggaataa tagcctgtat gcacaggctt atcgagctta tttagagcat
     1621 cctgtcaaag gttatcctgt tgctctttta aattcctgcc taaagaatgt ggtatttttt
     1681 cccgcaaaat atgggagtga aagggcgctt tatgcgcttt cagggaatga acagcaagca
     1741 aaaaattatt ccggattcct ggccgggctc atgaccgttt acctcactgc acctatttct
     1801 gttgtgaaag caagactgat gactaacgta ccccttaata cgcttagttt aaaacgattg
     1861 ggctcaggcg tgaatgccat cgcgatgcgt gactgcattc aatatggaat ttattttaat
     1921 gccaaagagc ggttaaatga aaaaattggt gacggtatca tggcgggtgg catagctggc
     1981 attgtggggt atgtctttag taatccctta agtgtgattg gactcaatca aaaaacggct
     2041 gttgaatcag taaacattcc caacatggcg aaacgaattt atcaggcgaa tggtgtgaaa
     2101 gggttttatc ctttagtcgg cctggccgct tggggcatgt ttgctcgtgg cgtggctatc
     2161 catcagggca ccaaactata cgaatcctta caggaaaacg aagcttatgg aaggccaaca
     2221 aggaacaggg agtaaattca taacgccccc tgtttccatg aaaatatttc agggggcagg
     2281 acgatgaatc gtattggaca ataaggctat aacgcggtga cggtttaata atagtctccg
     2341 tattgactca attacgggca agcaaggtct ggccttggcc cgaccttgct taaatttcta
     2401 aaaaacacac tattattcct aattagttat agtatacttt aactaaacaa tcgttttagt
     2461 gttttttatg aaaatttcta ctaatgaatt aattgcagta gtatcccaat ttaatccctg
     2521 gtggagaggt gaatccattc aggatttacc tacttggcat agagcagctt accaagagtt
     2581 ggaattatgg ttaattaaac cacctgtacc tagagcaata tttttgtctg gagctcgaca
     2641 gataggtaaa acaacccttt tgttacaagc tattgataag cttttgaaag aaggtgtaaa
     2701 gccaggaaat attttgtatg taacctttga tcatccatta ataaaaatgt ctgatattga
     2761 tgctgtatta gaggcatgga gggaacgaga acctaaaaca ggtgatttag aatatatttt
     2821 tattgatgaa gctcaattca tgcggagttg gggaacatgg attaaacacc aagtcgattt
     2881 ttcaaagagt agaagaattt tttttacggg atctgcaaca ccaattatag aagcagatca
     2941 agagtcaggg gtagggcgtt ggcatactat tcgtctaaca acactctcat tttatgaata
     3001 tttacaaata aagaagattg atttgcccca gttaccaact gttaagacct tgcgagaatt
     3061 attcgagtgg ccacaacaag aattttataa ggttaaagaa ttagcggttc attatgtagg
     3121 acattttcat gagtatttat tacgaggtgg atttccacaa acagctcaaa ttgaaaatat
     3181 tacccaggca cagcgccttt taagagaaga tataattgat aaagtactaa aaagagatat
     3241 gacagcactg tttggtgttc gtcgtatttt agagttagag caaaccttcc tttatctatg
     3301 catgcatgat gggggattgc tagatatgac cgatctttgc agtaatcttg aagtaaagag
     3361 acccacaata caaaattata tttttttgtt ggaggctaca aatctcattt atcgcttacc
     3421 tccctttggt tacggtaaag atattttgag agcaaaatat aaaatttatt tagcagacgc
     3481 agctatagct ccagctgtat tactgaaaga taaaagtatt ctggatgatc cagaatcctt
     3541 aggtattgcc acagaaacag ctgtgtttaa acatctattt gctcgatatt actcgcaaaa
     3601 tgtacggttt agttattggc gcggcaccaa agaccatgaa gtggatgttg tagcacagat
     3661 tggtgatcaa ataattcctt ttgaagtcaa atatcgttca caacatacga atgccaaagc
     3721 attgaaagga ttgttagaac tatgtaagaa aaaaagtatt gctcatggtt atgtagtaac
     3781 taaatcgtta gatgacttcg gtattatgaa agccctacca gaaattaaga caaatgttat
     3841 gcagataccc gcaccattac tttgttactg gatgggagaa tcagaattaa ggcagacatt
     3901 tggggtttaa ttagttgata acttaatgcc gtcaaataat ttattgaaca ctgacgcaaa
     3961 ataacttatg gcaaatattt atccctcaaa atgtttctca ataagtcatt gctaccttga
     4021 accagggtaa actttaattt tcttaaacaa aatggagtat ttagtcctcc cttagatgga
     4081 ggctctttca ctgctttgct gagcaaatat ttgacgctcc tcactgatta attcaatcca
     4141 ttttttaggt gtcatgtcgt tttgtatcaa taagttgatc gctgcactag gatattgact
     4201 ttgtgtaatg gcaaaataaa agtaagcggt caatttgctt ctgcataaag cccaaccact
     4261 ttttgcgaca cattgttgca aactagcgaa ttcaatttga tcttctttga ctaattgttg
     4321 cagcttttga atcgtgttag ggatacatgc atcaaaaaac aatgtggtcc cttgctgtcc
     4381 ccatacgtca cttaatataa ttctttttaa aatgttcatg tcacaaattt gttgctcttg
     4441 tgaaataggt ttctcaattc tatgtgttac cggttgtccc aacatggaat gtgtccattg
     4501 ttgataataa gcccggccgt aaattaatat accacccaaa ataaaatcaa aggcagttgg
     4561 ggctgcatac caaagagcag tgaagaaatt ggatttgatg acgtccactt tggggtaggg
     4621 atactgatgg ttattggttt taatgatatg tttgcgatcg tattgcattt ttaaaggttc
     4681 atatttaacc aaggatttag cctggtattg attctgacca atgcttatcg tttcttcctt
     4741 cttcggtact cctccatcga ctattgctaa atgaaagtta tgatttaaaa catgttcata
     4801 acccagaaaa tgacaatcag gatttttaga taaggtattt gctaaatgtt gtcgcaatgc
     4861 ctccccaact ttaatattag gatcataaaa atctggatgt ttggctaaat tcattaccca
     4921 aaatacattg actttatcct tttcaccagc aggaatatct cgtaacatca ttccccaaca
     4981 atacccaata acttcgtcgt ttttatcctt ggcaataaaa caaattgtct ctttataatg
     5041 taacattgaa gataggacga tatcccctat aaaaggcaaa tcgccaaaat ttgctccatg
     5101 ggctaaagaa tcgatacgtt taatttcccc cattgcttta ttaagttcag cggtattatt
     5161 gggatctaaa atctgaactt ctttgaagcc atagggctta ccttccatca tttttaacga
     5221 aaaattaaag gtacattcgc catctaactt tttgtggtgt ttaattttaa gaggattttg
     5281 atttctgcaa tttaacagat ctgtccataa ttgatgaatg taaagtaatc tttcatcatt
     5341 tggtgaggca ttttgcaaat tgattaattg ctgggtgaaa cgatgcacat attcggtatg
     5401 aagataaatg gcatggctac tgaaatcgac aacaaactct gccgtcatta attttttgat
     5461 ttcgggtaag tgatattcaa caggatctgt atatgatttt actattgctt ctagtgaact
     5521 ccatttgcgt tcaagtacat cgatttttga atacgacatc tatatttcct tgtaataaat
     5581 tttcggagaa atcttaacag ggcaatttac ttggccaaat cctatttggt caataaaaat
     5641 taggcctttt taaatttaaa aggagctatc ttcatatgga aaaactataa ctctcgagac
     5701 aggagttttc attgtgctgt gttgacatta gacagagatc gccagcgatg gggtgagggc
     5761 cttggatcgg attcctattg acacaagctt tctaaaggaa tagttgagaa tttagagtac
     5821 tgatatcttt cattgccagt ttgggctgga tatgcgagaa aacaaagcct tacaatacaa
     5881 taaggttggt cgagggggaa ttgaacgcca caatccatgt ggcttccatc ttgcctgatg
     5941 ctccaattcc gcctcctcgt ttttttatca taacaaattc tagaatggag tcacgggtat
     6001 aaagtctgaa tggtgtgtaa gatatttctc attctctgct acttacatga tcataaatag
     6061 ccaggttgta ttatttattg ttttacaata tctccaggtt aaagaaatct atctatttca
     6121 atctctttat tatgatttcc actttgcaaa aagaacaagc atccttctaa gtgctggaat
     6181 ttagggtata accagagtgt attcacagag ttatccacag aaaatgggga taagtgatta
     6241 atgcaatcca ggtatcaata tctaataagt ccttgtaggg caaggggttt tgtaaaatgt
     6301 gcaggagctc aggcggtcag agattgaaaa atggaaatag ttattcacag gggggcggtg
     6361 caatttagat gttaagcgat ttacatcgta acaaaaaaaa aaataaaaaa acagtcttga
     6421 cgaagtcgat tttttgcaaa aac
//
LOCUS       DBNCWK010000148         6426 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.148, whole genome shotgun sequence.
ACCESSION   DBNCWK010000148 DBNCWK010000000
VERSION     DBNCWK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.148"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            223..1485
                     /locus_tag="AC06IO_002536"
     CDS             223..1485
                     /locus_tag="AC06IO_002536"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIO9875704.1"
                     /translation="MQRLKNIFTAIYQYLLKETEDASHQITLFGIVMMINYPLFGVFW
                     KLEHFQLTEEFILRITAALLCACLAFNQFWPRQLLKFLPVFWYIVLLFCLPYFFAYLT
                     LINNGSTLWLMNCVSAIFFLLLVSSVLGALVLLVSGVGLAFFHFYILSNNQFVYIPGT
                     ISLFSLIVTFIAAIIIGALFARDREITYAGRLSGMRMLAGSIAHDLRTPLASIYLQAE
                     LQELIVERLNNPEVQKDLKENLSKITRGIEMSNQLIRMQLNNIQRDKLDTSTFSIYSI
                     KKLLKASLEEYPFKENQKSLIHLNDKNDFSIWIDEVGFKNMMWNLLKNSLEYIEETHK
                     GEISIWLEQGWEKENFNYLHIKDTAKGLNHKEYEKIFDFFYSGRKEGTGLGLAYCKLL
                     MQAAGGDISCKGKKNEFAHFTLKFPKID"
     gene            complement(1533..2228)
                     /locus_tag="AC06IO_002537"
     CDS             complement(1533..2228)
                     /locus_tag="AC06IO_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIO9875705.1"
                     /translation="MPEETSTNLPKSLSHFLIQSDLDYKNSLSSIPLFDVKRTIFWDD
                     NLRKMFASIFYHLRGHFINFLWYVANFADNEASKQIIIQNIYEEIGIGNRFSHEKLYE
                     RFAKECGVDIHDEIVNETNYIPFAKEFNKAHLSWLAQHDPKDHLCAFAAYERLDNLDY
                     PFLVELAKSMKISQHGMTFFNVHTHVDHFDTTLQLIVPIWEESPQRLIESFHFIQTHQ
                     LGMWEQFSNALFN"
     gene            complement(2625..5048)
                     /gene="sdjA"
                     /locus_tag="AC06IO_002538"
     CDS             complement(2625..5048)
                     /gene="sdjA"
                     /locus_tag="AC06IO_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdjA"
                     /protein_id="HIO9875706.1"
                     /translation="MFSYLDKLLDGIFGYQKSDTSQLSTSTLPTLTTVPLKQEYFVKI
                     GESETQDLGLLPVVSKRNNQSVPLEEIPFEDTRLELIELYIALLKQCVLDEKLKSIPA
                     QYLISHYLFIKTLAANEGNKGRKDLYLNLSQKVADYLEKNESKIWSMAVACTKTSEYP
                     IVDWIKKHHLHFNFIRAFILDYNKKPLTHDQRAFMQQFRDSAAFFFPDQVYLAWLTQS
                     YEPGSILNPMYRESRSTHYYHANITDNLLLRTRPKQVNFGPQHFFQKGKGSVKNTYRF
                     NINDGKLMRIQGRTLLFSTNKDNEVIAVKVQKKGEPQSALSNEFQMADYLLKHQRRLK
                     LQSQLPTPLSQYSINRTEILKKCSKSLDFEKFKNLISDAKSLEIYVYKATPSYFTYLH
                     DKQQSFSQLTSSVQKNVHDLFVLLREGIVFPYLADMFHTHIDESKRSDKGRYQTLVEL
                     LSALQSQMGRLDKWQKAVEFVNLRASGIADLGDNLPLTSFLTVSDWTKHYHAELLTGV
                     YHPSFLFLDKSSGSVRSLFNSRRKVFGNYLYLNIIAEYLLVIQLVIGCYGDKVTRKMD
                     SKSKAKVWEHLAELMFSSCAEAVNLITGMPKSRALAFLKQRANVRKHTQQTSFWMTPD
                     YSNLDRLTVQSQQSTLYPGESDYEINSDLIPGIGLSLDGINQDLGDYNQASPLRELEK
                     LLYATVTLVEGTQQLDKQFFQQLDETEKMILSAAGVDKCYQAVAKLLDLARPGCQMQR
                     RLAFTYYEMIKRIYPCSNPADVRFELVAKEEAIIKIQRFWREHKKENQSLEKGFDFDR
                     NTSSSQSPL"
     gene            complement(5119..6315)
                     /gene="dupB"
                     /locus_tag="AC06IO_002539"
                     /gene_synonym="laiF"
     CDS             complement(5119..6315)
                     /gene="dupB"
                     /locus_tag="AC06IO_002539"
                     /gene_synonym="laiF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase DupB/LaiF"
                     /protein_id="HIO9875707.1"
                     /translation="MLYNKSSFCLWKETIVPIILDSDVLEVAEYVYKTRLSQPYTEVG
                     SEWEYNYKNPTATFAKGDGHNLQRYITIDGKQLHRPIHGLAHTMRTLMYSQLMYCSSK
                     KQLSPHVCQDGRTIADLSELDLKKINIAQLFFVAGRESEASYGDAYHRYHLYGAKQFE
                     EYARKHLTHLFSEEEIQLYSRCIEDRVGDSFDGTPEGYIIHLSHMIDLMRCKSPVEVF
                     LGHSKGVSGIVPTLIHLFGKQDGLDIMHYARGLFAATGEAVPYIDSSEWSHLGVDLSR
                     VQRAHSIVGDINVPGQEADSKKTAQAGFSVDGCYSALTSVPTPSWYEKELKEIDDEKI
                     DVKEVDDREIEKEHEIVVPSQATTPLSTVKTDSFVEKLLKPFMIWKKPEEVQTTQPTT
                     EKTNKP"
BASE COUNT         2030 a         1207 c         1105 g         2084 t
ORIGIN      
        1 gtatccttgg tatttgtttt aattgggagc aatattattt aacttaagtc caagtaaccg
       61 tagaaatcat aactctaatt ggtagcatcc attcagagtt caccattgaa atactcataa
      121 tttacaagta tttctaatcc tacactaact gttcaatgtt caggactaat aagattaata
      181 cccaaacaat ctcaaagttt ttactcagat aattaaggaa tcatgcaaag gttaaaaaat
      241 atatttactg caatttacca atatttatta aaagaaactg aagatgcaag ccaccaaatc
      301 actttatttg gcatcgtcat gatgattaat taccctcttt tcggagtttt ctggaaactg
      361 gagcattttc aactgacaga agaatttatt ttacgcatca ctgcagccct cctctgtgcc
      421 tgtttggcat ttaatcaatt ttggccaaga caattattaa aatttctacc tgttttttgg
      481 tatatagttc ttctcttttg tttaccttac ttttttgctt atttgactct cataaacaat
      541 ggctcgactc tttggctaat gaattgcgta tccgctatct tttttctttt attagtttcc
      601 agtgtgttgg gagctcttgt attgcttgta tcaggtgttg gacttgcttt ttttcatttt
      661 tatattttgt caaataatca gtttgtctat atcccaggaa ccatatcact ttttagttta
      721 attgttacgt ttattgcagc aataatcatt ggtgctttgt ttgcacgaga tagagaaatt
      781 acctatgccg gacgtttatc gggtatgcgc atgctggctg gaagtatcgc tcatgattta
      841 agaacgccgc ttgccagtat ttatttacaa gccgaattac aggaattgat tgtcgaaaga
      901 ttaaataatc cagaagttca aaaggacctg aaggaaaatt taagcaaaat tactcgcggt
      961 atagaaatga gtaatcaatt aattcgtatg caattaaata atattcaacg agacaaatta
     1021 gataccagta cattttcgat ttattccatc aaaaaattac tcaaggcgtc tttagaagaa
     1081 taccctttca aagaaaatca aaaatcattg attcatttaa atgataaaaa tgatttttcc
     1141 atatggattg atgaagtggg ttttaaaaat atgatgtgga atttgctaaa aaatagtctg
     1201 gaatatattg aggaaaccca taaaggcgaa atctctatct ggctagaaca aggatgggag
     1261 aaagagaatt ttaattacct ccatataaaa gatacagcta aaggtttaaa tcataaagaa
     1321 tatgaaaaaa tatttgattt cttctattct ggtagaaaag aaggaactgg tttaggcttg
     1381 gcctattgta aacttctgat gcaagcagca ggtggtgata tttcctgtaa agggaaaaaa
     1441 aatgaattcg ctcatttcac tttaaaattc cccaaaattg attaattcct atttaacata
     1501 agaaatcccg agatttctta tgttaaaaaa tatcaattaa ataaggcatt ggaaaactgt
     1561 tcccacatcc ctaactgatg agtttgaata aaatgaaatg attctatcag acgctgaggt
     1621 gattcttccc atatgggcac aatcaattgt agcgtggtat caaaatgatc aacatgggta
     1681 tgtacattaa agaaggtcat gccatgttga gatattttca tcgatttggc cagttcaaca
     1741 agaaaaggat aatcaagatt atccaagcgc tcataagccg caaaagcaca caaatgatct
     1801 ttaggatcat gttgggccaa ccaggataaa tgagctttat taaattcttt ggcaaaagga
     1861 atgtagttgg tttcattcac tatttcatca tgaatatcaa caccgcattc ttttgcaaaa
     1921 cgttcataaa gcttttcatg ggaaaaacga ttgccaattc cgatttcttc ataaatattt
     1981 tgaataataa tttgttttga agcttcgtta tcagcaaaat tggcaacgta ccacaaaaaa
     2041 tttataaaat gccctcttaa atgataaaaa attgatgcaa acatttttct aagattatca
     2101 tcccagaaaa tagttcgctt aacgtcaaat aaaggaatcg aactcaatga gttcttataa
     2161 tccaaatcag attgaataag aaaatggctg agtgattttg gtaaatttgt gctcgtctct
     2221 tcaggcatga ttcacacttc atatgaaata agttaagtcc agtaagataa ttaaacatta
     2281 taccatttga acattattta atcaataaga tattattgac cgtttaaaaa aatttgcttt
     2341 ctttataaaa gcaaaagtat cgttttttgt cttgtttttg tcaattcaaa gcgtaatcat
     2401 atctgatatt gagtttaaac ttccaccata attccctgga tgaataataa aattttgtct
     2461 taattaaata gcataaaatc gaattgttag cggaaataag aaaaatctcc ttaagcttat
     2521 gagtactttt aattttccaa aattaaaaat aacaggtcag tgccctgagg ttgaaatatc
     2581 ctctaacgat cttggggtga tctcttacaa ttgaaatgac tttttcataa aggcgactgc
     2641 gatgaactag tgttcctgtc aaaatcaaac cctttttcaa gcgattggtt ttctttttta
     2701 tgctctcgcc aaaaacgctg aatttttata atcgcctctt cttttgcaac aagttcaaat
     2761 cttacatctg caggatttga gcagggatat attcgtttga tcatttcata ataagtaaaa
     2821 gccaatcttc gctgcatttg acaaccaggc cgagcaagat ccaggagttt tgcgacagct
     2881 tgatagcatt tatcaactcc agcagcactc aaaatcattt tctcagtttc atccaactgt
     2941 tgaaaaaatt gcttatccag ttgctgggtt ccctctacta aggtcactgt agcatacaat
     3001 aatttctcta gctcccttaa agggcttgcc tggttataat cacctaaatc ctgattgatg
     3061 ccatcaagac tcagacctat ccccggaatt aaatcactgt ttatttcata atctgattcg
     3121 ccggggtaca aagtggattg ttgagactgc acagtcagcc tatccagatt cgaataatca
     3181 ggggtcatcc aaaaagacgt ttgttgagta tgttttctta cattggctct ttgctttaaa
     3241 aaagcaagtg ccctggattt gggcatacca gttatcaaat taaccgcttc agcgcaactg
     3301 gaaaacatca attcggctaa atgctcccaa accttagctt tgcttttact atccatttta
     3361 cgggtcactt tatcgccata acagccaatt accaattgaa taactagtag atactcagcg
     3421 atgatattca gatacaaata attgccgaat actttgcgcc ggctgttaaa caaggagcga
     3481 accgagccac tggatttatc gaggaagaga aaggatggat ggtaaacgcc cgttagcaat
     3541 tccgcatgat aatgtttcgt ccagtcagaa acagttaaga agctggtcaa cggaagattg
     3601 tcacccaaat cagcgatacc gctggctcgt aagttaacaa attcgactgc tttttgccat
     3661 ttatcaagac ggcccatttg tgattgcaaa gcacttaaaa gctcgactaa cgtctgatat
     3721 cgacccttat cgctcctttt actctcatca atatgggtgt gaaacatatc tgccagataa
     3781 ggaaagacta tgccctctct caggagcacg aaaagatcat gtacattttt ctgcactgat
     3841 gaagtgagtt gactaaagct ctgctgctta tcatgcagat aagtaaaata agaaggtgtt
     3901 gctttataga cataaatttc aaggcttttt gcatcactta tcaaattctt gaacttctca
     3961 aaatcaagtg atttactgca ttttttgaga atctctgttc gattaattga atattgactg
     4021 agaggggtag gtaactggct ttgcaatttc aaacggcgtt gatgtttcaa caaataatct
     4081 gccatttgaa attcattact taaagcagat tgcggttccc cttttttttg cacctttaca
     4141 gcaatcactt cattgtcttt attcgtgcta aaaagtaatg ttcttccctg tatgcgcatt
     4201 aattttccgt cattgatatt gaaacgataa gtattcttga cggagccttt tcctttttgg
     4261 aaaaaatgtt gaggaccaaa attgacctgt ttggggcgtg ttcttaacaa gagattatca
     4321 gtaatgttcg cgtgataata atgcgttgat ctgctctccc tgtacatagg attcaagata
     4381 gagcctggtt cataggattg ggtaagccaa gcaaggtaaa cctgatcagg aaagaaaaaa
     4441 gcagcagaat ccctaaactg ctgcataaac gcacgttgat catgagtcaa tggtttttta
     4501 ttataatcca gtataaaagc tcttataaaa ttaaaatgaa gatggtgctt ttttatccag
     4561 tcaactatgg gatactcact ggttttagtg catgcaacag ccatactcca tattttagat
     4621 tcattttttt caagataatc agccactttt tgagataaat taagatacag atcttttcga
     4681 cctttatttc cctcgtttgc ggctaaggtt tttataaaaa gataatgaga tataagatac
     4741 tgcgcaggga tactcttcaa tttttcatct aaaacacatt gttttaacaa tgcaatataa
     4801 agctcaatca actcaagtct ggtatcctca aaaggaattt cctccaaggg aactgattga
     4861 ttatttcgct tgctaaccac tggcagtaag cccagatcct gtgtttcact ttccccgatt
     4921 tttacaaaat attcttgttt caagggaaca gtagttagag taggtagcgt tgaggtgctt
     4981 aactgactag tatctgactt ttggtatcca aatattccat ctaataattt atccagataa
     5041 ctaaacatag ttacaacacc tcattgcgtt ttaattccct aggagaatcc gtttctctat
     5101 tactttaaat aaaacaggct aaggtttatt agtcttctcc gttgttggtt gagtcgtttg
     5161 tacctcctct ggctttttcc aaatcataaa tggcttcaga agtttttcga caaaggagtc
     5221 agtttttact gtacttaaag gtgttgttgc ctgcgaagga acaactatct catgttcttt
     5281 ttctatctcc ctatcatcaa cttccttaac atcgattttt tcatcatcga tttcctttaa
     5341 ctctttctca taccaggaag gtgtaggcac actggttaac gcagaataac aaccatccac
     5401 agaaaaaccg gcttgcgctg tttttttaga atcagcctct tgcccaggga cattgatatc
     5461 accaacgata gagtgtgcgc gttgtacccg actcaagtca actcccaaat gagaccattc
     5521 agatgaatca atatagggca ccgcctcacc tgtagccgca aacaagcctc gggcgtaatg
     5581 cataatatcc aagccatctt gtttgccaaa caagtgaatc aatgtaggta caataccaga
     5641 aacccctttt gagtggccca aaaatacttc aaccgggctt ttgcaacgca tcaaatcgat
     5701 catgtgtgac aagtgaatta tataaccttc aggagtccca tcaaaactgt cacctaccct
     5761 gtcttctata catcgtgagt acaattggat ttcttcttcc gagaaaagat gagtcaaatg
     5821 cttgcgggca tattcttcga actgtttagc cccatacaga tgataacgat gataagcatc
     5881 accataagaa gcctcgcttt cgcgcccggc tacaaaaaat agctgcgcaa tgtttatctt
     5941 tttaagatca agttcactga gatcggctat tgttcgtccg tcttgacata cgtgtgggga
     6001 aagttgtttt tttgaggagc aatacatcaa ttggctatac atcaatgtgc gcatcgtatg
     6061 agctaagcca tggatagggc gatgaagctg cttgccatca attgtaatat acctttgcaa
     6121 gttatggcca tcgccttttg caaaagtggc tgttggattc ttgtaattat actcccattc
     6181 agaacccact tcagtgtaag gctgagataa gcgtgtttta tacacatatt ccgccacttc
     6241 caacacatca gaatctagaa taataggcac aatggtctcc ttccataaac aaaatgagga
     6301 tttattatat aacaagtgta attatttatc aattcattgc aataggtata aaccaaaaaa
     6361 ttgcaatgat ttaatccaaa gtctcatatt cgattgtttt agataactca aattgtgaac
     6421 tcaatt
//
LOCUS       DBNCWK010000149         6400 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.149, whole genome shotgun sequence.
ACCESSION   DBNCWK010000149 DBNCWK010000000
VERSION     DBNCWK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.149"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(109..1299)
                     /locus_tag="AC06IO_002540"
     CDS             complement(109..1299)
                     /locus_tag="AC06IO_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIO9875708.1"
                     /translation="MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFNTDS
                     TTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSTALLFFAGSLISALAPSVPALI
                     FARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDM
                     TQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPELPQMRVHQVMRQIISNSDFRQYT
                     WVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMTVFVANMMAARLTYSHS
                     LESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGTATALALKDF
                     EQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRI
                     LSDF"
     gene            complement(1566..2117)
                     /locus_tag="AC06IO_002541"
     CDS             complement(1566..2117)
                     /locus_tag="AC06IO_002541"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIO9875709.1"
                     /translation="MFNVAVIVGSLRKESFNRKLAFALDKLQHPTFQLSHIKIDDIPL
                     YNQDFDNNLPDSVIRLKKEITEADAVLFVTPEYNRSIPGVLKNIIDWGTRPYGKNVWA
                     GKLMAAIGTSPGAIGTAVAQSHLRSIMVAIDGVCFGQPEIYLVYKDGLIDNNFSITMD
                     STRNFLQGFLDRFAREIEKYKSI"
     gene            2268..3227
                     /locus_tag="AC06IO_002542"
     CDS             2268..3227
                     /locus_tag="AC06IO_002542"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIO9875710.1"
                     /translation="MGILIDGIWHDENNINTDKEGRFVRAESGFRQHITANGENGFKA
                     EPDRYHLYLSYACPWACRTLIMRQLKGLEKIISVSFSDPIMGKNGWSYGESGNETADE
                     INHIHYLKELYLVARKDYTGRVTVPVLWDKKKKTIVNNESSEIMRMLNSEFNAFSSTD
                     HDYYPKQWRQKIDEINQLVYENINNGVYKCGFAKSQKAYEEAFDALFNTLNQIENLLA
                     TQRYLIGEQLTEADWRLFTTLIRFDAVYHGHFKCNLQQIADYPHLSNYLRELYQFPGI
                     KETVNFNHIKQHYYRSHLSINPTGIVPKGPKLNLDEPHNRYEF"
     gene            3266..3739
                     /locus_tag="AC06IO_002543"
     CDS             3266..3739
                     /locus_tag="AC06IO_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIO9875711.1"
                     /translation="MQKITPCLWFDDKAEEAVTYYISIFKNGSIEKITYYGKEGVEFH
                     KKPTGSVMTILFNLNGQQYVALNGGPEFRFNESISLMVTCDTQDELDYFWKELSADGE
                     EGPCGWLKDKFGLSWQIVPNILAELLSDHQKSEKVMRVLLQMKKLDIAKLKAAAE"
     gene            3840..4250
                     /locus_tag="AC06IO_002544"
     CDS             3840..4250
                     /locus_tag="AC06IO_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIO9875712.1"
                     /translation="MKPLIPYLLFNGTCREAMTFYQNCFGGELELMTYGDSPEGACDK
                     KADKNEIMHACLRQGGFTLMASDWPGGEAKQGNNVHLNIDCSTKLEIEALFKKLSQEG
                     TVVQALADTFWGAHFGMLIDRYNIHWMLNYSLKQ"
     gene            complement(4374..4931)
                     /locus_tag="AC06IO_002545"
     CDS             complement(4374..4931)
                     /locus_tag="AC06IO_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2443 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875713.1"
                     /translation="MIRKSEMIRHFFIVLFFFVGFMNQASALPWFENENDLRNNNKLR
                     YRAHDEIKKNQIDFSGRWSGQCDSNPAVDLTIKHTREHISISYGFMEEKYIPGEVRAE
                     SSSRLSLSESGSTTVKWNQDNTALIFINYHLFINESGSLNVFFSKVSMALEQEQLVVK
                     GQHFQTGNTLDDIKQETISCIYDRK"
     gene            complement(5158..5772)
                     /gene="mavI"
                     /locus_tag="AC06IO_002546"
     CDS             complement(5158..5772)
                     /gene="mavI"
                     /locus_tag="AC06IO_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavI"
                     /protein_id="HIO9875714.1"
                     /translation="MYSVKLCFALFRLYCYWRKLSMGLSAQQKTAIKSYFESNYNLFF
                     GIPTERKEGDLTPNEHVMVLQYLRFYLLKLPIECLEEELVNKQINGDDTVGILLEDFL
                     GNTLSPMNPDAYFHSQKNFIQMGNAEDPAKPEDIIQYLIEVIENMSQQMDHGYLKKES
                     DPRIEKSNSSYASRALKWGLFGGAALAITYAVSKLSDKSHTNSL"
     gene            5919..>6400
                     /locus_tag="AC06IO_002547"
     CDS             5919..>6400
                     /locus_tag="AC06IO_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875715.1"
                     /translation="MNRFIRFLLLLLITCSSGSFNLYAANKNNDFVLFEKIFTDWTLA
                     FNQKKLAPTCALFSKSLAADYQGAPQKNYTSICHGFKKIFQQTRKNYQYHFKLKRVYR
                     SGHLAVARITWYLQIFEQGKLISETQDEGLDVFLKDSQNQWKIINFIAYPVPDNLNEK
                     "
BASE COUNT         2189 a         1228 c         1142 g         1841 t
ORIGIN      
        1 gtgattacga ttaaattttg ctgccaattc tgctcggttt tcaccttaat ttcaatttaa
       61 ataaataggc taaagctacc cgaattgtag aatagcattt tgaatcaatt aaaaatcgct
      121 taatattcta aaactgaatt tggtgattct agccaatccc actgcagtga aacaaagtac
      181 ggggatagct acacttaatg gcccgggcat ccattgcgct gtcaaaacac caatcagacc
      241 cgcgagacca aactggcaag cacctaacag agcggtagct acaccagctt gctgttcaaa
      301 atcctttaaa gcaagtgctg ttgctgtgcc catgctaata ccaataccca cagtcaagca
      361 aagcatggga agcataaaac ttaaaatgct gcattgacga agattaagag ccagcataaa
      421 aaagcatgac agtgtcatta aaaccaagcc ccaacgcact atgctctcca atgagtgaga
      481 ataagtaagt cgtgcggcca tcatattggc aacaaacaca gtcattgcat tcaaaccaaa
      541 ccagaaacca tattcagtcg cactcaggtg cagttgtgtg atcaaaatac ctggtgattg
      601 agcacaaaac aagtacaatc ccagcaaact ggctgatgca acccatgtgt attggcgaaa
      661 atccgagtta cttattatct gcctcatcac ttggtgcacc ctcatctggg gcagctcggg
      721 tttgggatag tgataatcag ggatgtaacg gaaggctatg ccactcatta ataacccaag
      781 caaggttaaa aagtagaatc cactatgcca atcttgcgtc atatccaaca ataatccccc
      841 aatgactgga gcagaactgg caaccatcga attcgtccca gacaggatac taaataagcg
      901 ggcacaggtg cccgttgaaa aattatctcg aacgataata aagcatagta aataggttcc
      961 acaggcacca gctgcctgaa tgatacgtgc aaaaatcaat gccggtactg acggagctaa
     1021 cgcagagatc aacgatcctg caaaaaaaag aagtgccgta ctcaaagcca tctgtcttcg
     1081 tccaaaacga tcggccaaag gcccaaccaa tagctgaccc aaagctacag tgagcataaa
     1141 caaataaagg gtagcctgca tcgttgtact gtcagtattg aaataacgac tcatttccgg
     1201 aattgcgggc acaaaaacat ccatagcaaa agaaatgctg ataatgagtg gaaataatcg
     1261 aaaaagactt tgtctttctg tccagccaat aaaattcaat taatggtgtt cctgttaaaa
     1321 tattacttat gaaaaatata aactgcgctt aattgtaaca gatttacact caatgttata
     1381 aaattgctga ttcaacagat gttattcaat taattattgg tagtccaaac gcaaaaattc
     1441 tggactacct taaaacataa ggaataacga agtgagcaag aggctttagc caaaatgaat
     1501 gatcgggaac atagaaaaat gcaccaattg ccagaaacat tgttttgggt aaccagtcga
     1561 catacttata ttgatttata tttttcaatt tccctggcaa aacgatccag aaatccttgc
     1621 agaaaatttc gggtagaatc catggtaata gaaaaattgt tatcaatcag cccatcttta
     1681 taaacaagat aaatttcagg ctgcccaaaa caaacgccat cgattgcaac cattatgctt
     1741 cgcaaatgcg attgagcaac agcggttcca atagctcccg gactcgttcc aatagctgcc
     1801 attagtttgc ctgcccatac attttttccg tatggcctgg tcccccaatc tataatattc
     1861 ttcaatacgc caggtatcga acggttatat tcaggtgtca caaataaaac agcatctgcc
     1921 tcagtaattt ctttttttaa ccgaataact gagtcaggta aattattatc aaaatcctgg
     1981 ttataaagag gaatatcatc tattttaata tgagatagct gaaatgtagg atgctgaagc
     2041 ttgtccagag caaaggctaa tttacgatta aaagactctt tacgaaggct tccgacaata
     2101 acagctacgt tgaacataaa tttttcctta aatttattga tacaaaatga attaatcgat
     2161 ttatcaatga ttatgcatta aaaaacagtt attaaacaag acgtattcat gctgcgccag
     2221 tattaaaaaa aaatattaat aaattattga tatataagga attcataatg gggatactaa
     2281 ttgatggaat ttggcacgat gaaaacaata taaacacaga caaagaagga cgttttgttc
     2341 gtgctgaatc aggtttccgt cagcacatta ctgcgaatgg agagaatggg tttaaagcag
     2401 agcctgatcg atatcattta tacctgtcct atgcttgtcc ctgggcatgc cgaacgttaa
     2461 tcatgagaca actgaaagga ttggaaaaaa ttatttctgt ttctttttca gatccaatca
     2521 tgggtaaaaa tggctggagt tacggcgaat caggtaatga aacagccgat gaaatcaatc
     2581 atatccatta tctcaaagag ttgtatttgg ttgccagaaa agattatacc ggtcgagtaa
     2641 cagttcctgt tttatgggac aaaaaaaaga aaacgatagt caataatgaa tcatcagaaa
     2701 tcatgcgtat gttgaatagc gaatttaatg cctttagttc tacagatcat gactattatc
     2761 caaaacaatg gcgccaaaaa attgatgaga ttaatcagct tgtctatgaa aatattaaca
     2821 acggtgttta caaatgcggc ttcgctaaat cccaaaaagc ttatgaagaa gcatttgatg
     2881 cattatttaa tactttaaat caaatagaaa acctattggc aacacagcga tatttgatag
     2941 gagagcaact caccgaagcc gactggcgtt tatttacaac attaattcgg ttcgatgccg
     3001 tgtatcatgg tcacttcaaa tgcaacttgc agcagattgc agactatccg catttatcca
     3061 attatttacg agagctttat caatttcctg gtatcaaaga aaccgtaaat tttaatcata
     3121 tcaaacaaca ttattatcga agtcatttaa gcattaatcc tacgggaatt gttcccaagg
     3181 gcccaaaatt aaatttggat gaaccacata accgctatga attctaataa atttttcaat
     3241 agctagaaat aaggataaaa aaaccatgca aaaaatcact ccttgtctat ggttcgatga
     3301 taaagccgaa gaagcagtga cgtattatat ctcgattttc aaaaatggga gtattgaaaa
     3361 aattacttac tacggcaaag aaggagttga attccataaa aaaccaacag gcagtgtgat
     3421 gacaatcctc ttcaatttga atggacaaca atacgtggca cttaatggcg gccctgaatt
     3481 tcgatttaac gaatccatct cactgatggt cacttgcgat acacaagacg agttggatta
     3541 cttctggaag gaactctcag cggatggaga agaaggtcct tgtggctggc tcaaagacaa
     3601 atttggacta tcttggcaga tagttcccaa tatcttggct gaattgctga gtgaccatca
     3661 aaagtcagaa aaagtcatga gagtgcttct gcaaatgaaa aaactggata tcgcaaagtt
     3721 gaaagctgca gcagaataag atgttaagga aaaatcacaa aatcgaagca ataaacgacc
     3781 tttagcaaag aaattgagag ttacaagtct tgcaataatc aatctatcaa ggagaataca
     3841 tgaaaccgct aataccttac cttcttttca acggtacctg tcgagaggca atgacatttt
     3901 atcagaattg ttttggagga gaattagaac taatgacgta tggtgattcc cctgaaggag
     3961 cttgtgataa aaaagccgat aaaaacgaga tcatgcatgc ttgcttaaga caaggtggtt
     4021 tcactctgat ggcatctgat tggccaggtg gcgaagcaaa gcaaggcaat aatgtgcatc
     4081 tcaacattga ttgcagcaca aaattagaaa ttgaagccct gtttaaaaaa ctaagtcagg
     4141 aaggaacggt agtgcaagca ctcgccgata cattttgggg tgcacacttt ggtatgctga
     4201 ttgataggta taatatacac tggatgttaa attattcttt gaaacaatag aaatcaaaat
     4261 acaaaaaagt ctgatcgaca atttttaatc aattgagatc gtttcactaa ccagtaaagt
     4321 aagtcccatg tgaccagcaa aaagctcagc atacagggat ttactttatg tcattattta
     4381 cggtcataaa tacatgagat agtttcttgt ttaatgtcat ccaaggtatt tcctgtttga
     4441 aaatgctgtc ctttgactac cagttgctct tgctccaatg ccatggaaac cttactaaaa
     4501 aacacattca aactaccgct ttcattgata aataaatgat aattaataaa aattaaagca
     4561 gtgttatctt gattccattt cactgtagtg ctgccgcttt cagataaaga taagcgagag
     4621 gatgactctg ctcgaacttc acccggaata tatttttctt ccataaatcc ataggaaata
     4681 gaaatgtgtt ctcgagtatg cttgatagtt aggtccacag ccggattact atcacattgc
     4741 ccgctccatc tgccagaaaa atctatttga ttttttttga tttcatcatg agccctatat
     4801 ctcagcttgt tattatttct taaatcattc tcattctcaa accagggaag agctgaagcc
     4861 tgattcataa aaccgacgaa aaagaataaa actataaaaa aatgtcttat catttctgac
     4921 ttccttatca gtaggcaggt ttgtacagaa taatactttt tctgtctcgc agaaaacgaa
     4981 tttataatat cattgcaatc tactttttaa ggagtatccg ggaaaatagc aattcagggt
     5041 ccttgctttt gacacaactc ttacaacata gcatggtttt gctttggtat aagacgtact
     5101 tcgagaaatc cgctggaaat tgatattccg ttaccccgaa accgaatatt gtcgctttca
     5161 cagactattg gtatggcttt tatcagacaa tttgctgaca gcatatgtta tagctaaagc
     5221 agctcctcca aataggcccc actttagtgc tctcgacgca taagaactgt tgctcttttc
     5281 tattctggga tcactttctt tttttaaata accatgatcc atttgttgcg acatattctc
     5341 gatgacttct attaaatact gaataatatc ttctggcttt gcaggatctt cagcattacc
     5401 catctggata aaattttttt gactatgaaa ataagcatcg gggttcattg gagaaagagt
     5461 gttgcccaga aaatcctcta ataaaatacc taccgtatca tcaccattga tttgtttgtt
     5521 caccaactct tcttccaaac actcaatagg caactttaat aaataaaatc gcaggtattg
     5581 caataccatc acatgttcat tgggtgttaa atcgccttct ttacgttcag ttggaatccc
     5641 aaaaaacaga ttataattgg attcgaagta agattttatg gccgtttttt gctgagctga
     5701 taaccccata ctgagcttcc tccaatagca gtaaagtcta aataatgcaa agcaaagctt
     5761 aacagaatac agagtgatct taaatctttc atgatgacct tacaatgaat tttatgtcca
     5821 aattataaat aatcaaataa aaacaacatt aagaatttgc tgtcattgtc tggaaggatt
     5881 tgttaagtta atcttttctt tgggcttcac gaatcaatat gaatcgcttc attcgttttt
     5941 tgttgcttct cttgataacc tgctcttcag gttctttcaa tctttatgca gccaataaaa
     6001 ataatgattt tgttttattt gaaaaaatat ttactgactg gactttggca tttaatcaaa
     6061 aaaaactggc accaacctgt gctttatttt caaaatcatt ggccgccgat tatcagggtg
     6121 ctccgcaaaa gaattacaca tctatttgtc atggctttaa gaaaatattt cagcaaacaa
     6181 ggaaaaatta ccaatatcat tttaaattaa aacgggtcta tcgctcaggt catcttgctg
     6241 ttgcaagaat tacctggtac ttgcaaatat ttgagcaagg aaaactcatt tccgagacgc
     6301 aagatgaagg attagatgtt tttctgaaag attcacaaaa tcaatggaaa attatcaatt
     6361 tcatagcata ccctgtccct gacaatctta atgaaaaaca
//
LOCUS       DBNCWK010000150         6249 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.150, whole genome shotgun sequence.
ACCESSION   DBNCWK010000150 DBNCWK010000000
VERSION     DBNCWK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.150"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..510
                     /locus_tag="AC06IO_002548"
     CDS             <1..510
                     /locus_tag="AC06IO_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIO9875716.1"
                     /translation="VYSLLSPAYAVSHPKSTVNDTAIQNEILVYINNYRQQHGLAPLK
                     MDNRIVKEAKIHSMDMAKHAIPFGHKYFKKRIDKLHTQIKNSNAGAENVAYNYKNAQD
                     VVKNWLRSPGHKRNIVGNYDLTGVGIARDEKGKIYFTQIFLKTGNSHYASRKPFPSIF
                     SGALFSKKA"
     gene            904..2037
                     /locus_tag="AC06IO_002549"
     CDS             904..2037
                     /locus_tag="AC06IO_002549"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIO9875717.1"
                     /translation="MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDF
                     ANEEFEKAGAIIFDSKSTLLKKTQILLCVNEPDPKNLNGLPPDSLIIGHIDNNPLSPL
                     VKWCLDQKMTLFSMNLIPRISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTA
                     AGMIQPAKALILGAGVAGLQAIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQE
                     QDSETAGGYATETSEEYKKLQAELIDQYAKIADIVISTALIPGRQAPVLLYKKTVEQM
                     KPGSVIVDLATSRGGNCELSVADKIIKHGEITIVGLSNMAGLIPATASDLYSNNLVHL
                     INLLAKNPSEFNLNPEDEIIQQAVLCHKGTYLPFQGVKEKQNA"
     gene            2030..2326
                     /locus_tag="AC06IO_002550"
     CDS             2030..2326
                     /locus_tag="AC06IO_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HIO9875718.1"
                     /translation="MPEIHTLDNPYITILTIFVLACFVGYYVVWKVTPALHTPLMSVT
                     NAISSIIILGALIAAGSELIGSITWLGGIAIFITSINIFGGFVVTQRMLRMYKK"
     gene            2350..3747
                     /locus_tag="AC06IO_002551"
     CDS             2350..3747
                     /locus_tag="AC06IO_002551"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIO9875719.1"
                     /translation="MNSIVPLFYLLAAICFILALKGLASPSTSRRGNLLGILGMILAV
                     GSTLVMPTTYHHILLITLIAGGGIIGTIIALKINMTAIPQLVAAFHSLVGMAAVLVAY
                     CAFLSPASFQIGTPGAIATASLIEMSLGLIIGAITFSGSVIAFLKLQGLISGIPIRFY
                     AQNYVNLLIGLTILVLLILFVANQNLFLFNLLAGLAFVIGVLLIIPIGGADMPVVISM
                     LNSYSGWAAAGIGFTLSNHLLVITGALVGASGAILSYIMCVGMNRSIFNVIFGGINSS
                     GNTKQAHSANELRNVRQANGEDAAFLLSNAKDVIIVPGYGMAVAHAQHAVKELVDALE
                     SRSIRVRFAIHPVAGRMPGHMNVLLAEANIPYDRVFEQSEINRDFATCDVAYVIGAND
                     ITNPAAKTDPSSPIYGMPVLEVEKAKTVLFVKRSLSPGYAGVENDLFFHDNTYMLFGD
                     AKVMTESIAKALGEI"
     gene            3882..4265
                     /locus_tag="AC06IO_002552"
     CDS             3882..4265
                     /locus_tag="AC06IO_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875720.1"
                     /translation="MLRKIGFTLLCAVATFSSSTYSSVTHTLQAGVTLEYEFPPNEPL
                     VFVNYLYWEVAADCKITSEDPSNELFAEALAKAGKINGVTLSKGNTMKVTVHSGDILK
                     LSAESGAKVQITNFGPHTLKATCTA"
     gene            complement(4331..5911)
                     /locus_tag="AC06IO_002553"
     CDS             complement(4331..5911)
                     /locus_tag="AC06IO_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIO9875721.1"
                     /translation="MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTV
                     KGRKADRHATSDWMEMEKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
                     GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIG
                     DSADELREEIELVKGASHGFNLEDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGP
                     QPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFVRVCSGSYKKGMKLSHLRIG
                     KEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAP
                     ELFRLVRLRDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRL
                     KHEYKVDCIYESVNVTCARWVYSEDDKAMSEFRTKAYDYLALDGGDMLMYLAPTKVNL
                     TMAEERYPKIKFCATREH"
BASE COUNT         1932 a         1288 c         1182 g         1847 t
ORIGIN      
        1 gtatacagtt tgctctcccc tgcttatgca gtatcacacc ccaaaagcac agtgaatgac
       61 accgccattc aaaatgaaat attagtttat atcaataatt accgacaaca gcatggcctg
      121 gctcctctca aaatggataa ccgcatagta aaagaagcaa aaattcattc catggatatg
      181 gcaaaacacg cgataccatt cggtcataaa tattttaaaa aacgtattga taaattacac
      241 acacaaatta aaaactcaaa tgcaggggct gaaaacgtgg cttacaacta taaaaatgcc
      301 caggatgttg tcaaaaactg gttacgtagc ccaggacaca aaagaaatat cgttgggaac
      361 tatgacctta ccggggtagg tatagctcga gatgaaaagg ggaaaatata ttttactcaa
      421 atattcttaa aaacaggaaa tagccactat gcctccagaa aacccttccc atcaattttt
      481 agtggtgccc tcttcagcaa gaaagcataa aaattaaaat agggtgttta acaaaatttt
      541 tgcaattttt aagtacaatt gagtggaatt aatgacaacg ttagcctaat attgaaacat
      601 agacggcaat cattgttcag ccaacagtac aattttggag gccagattga gagtgtggtg
      661 agcaagctca gcctagactg agaagccaaa agcactccgg aggaatccac agttttaaac
      721 aggactgcac tcaatcttgc tgtctataac atactaacac catggggcgt attcaatacg
      781 ccctttttat tggttcattc ctcgcttgtc cggatccaaa gatccaaaat tggtatttta
      841 tgctgtagaa aattattcta aattacaata ggatagaata ataacggtaa taaggatttt
      901 cccatgatga ttgcaacctt aatggaatca gctcaggaaa cgcgagtagc tattactcct
      961 aactcggcca aacaatacat aaaactaggc tatgatgtag ggattgaaaa aaacgcgggg
     1021 cttagctcag attttgctaa cgaagaattt gaaaaagctg gcgcaataat attcgatagt
     1081 aaaagtacat tactcaaaaa aacacaaatt cttttatgtg ttaacgaacc tgatcccaaa
     1141 aacctgaatg gcttacctcc agattctcta ataattggac atatagacaa taacccactt
     1201 agcccgcttg ttaagtggtg tctggatcaa aaaatgacat tattttcaat gaatctcatc
     1261 cccaggataa gtcgtgctca aagcatggac agcctgtcgt ctcaggctaa cctggctggt
     1321 tacagggcag ttttggaagc tgcggcaaca tttcatagag ctatacctat gatgatgaca
     1381 gcagcaggca tgatacaacc ggccaaagca cttattcttg gtgccggagt tgcaggcttg
     1441 caagccatag ctactgcgaa acgtttaggt gcagtggttt atgcctttga cgtaagaaag
     1501 gcagccaaag aacaggttga aagtttaggc gctgaattca tagaggtaat gcaggagcaa
     1561 gatagtgaaa cagctggggg atatgccaca gaaacaagcg aagaatataa aaaacttcag
     1621 gctgaactca ttgatcaata tgcaaaaatt gcagacattg taatttctac tgccttgata
     1681 ccagggcgtc aagctccggt tttactttat aaaaaaactg tcgagcaaat gaaaccaggt
     1741 tctgtcattg ttgatctcgc aacgtctcga ggtggaaatt gtgaactcag tgttgccgat
     1801 aaaatcatca agcatggtga gattactata gtgggattaa gtaatatggc aggtctcatc
     1861 cctgctacgg caagtgacct ttattctaat aatctggttc atcttataaa ccttttggca
     1921 aaaaatccat ctgaatttaa cttaaatcct gaagatgaga ttatccagca agccgttctt
     1981 tgtcataaag gaacttacct gccctttcaa ggagtaaagg agaagcaaaa tgcctgagat
     2041 tcatacactt gataatcctt atattacaat attaaccatt ttcgtactgg cctgttttgt
     2101 aggttattat gtggtttgga aagtaacacc ggctttacat acacccctaa tgtcagtaac
     2161 caatgccata tccagtatta ttatacttgg tgctttaatt gctgcaggaa gtgaattgat
     2221 tggaagcatc acctggttag gtggcatagc catatttatt acttcaatca atatttttgg
     2281 tggctttgta gtaactcaac gcatgcttcg catgtataaa aaataatgat ttaaaggacg
     2341 gaatcagtaa tgaattcaat agttccactg ttttatttat tggcagccat ttgctttata
     2401 ttggctttaa aaggactggc cagtccctca acttcaagaa gggggaacct tctaggcatt
     2461 ctgggcatga tcctggcagt gggctccacc ctggtgatgc ccactaccta ccatcatatt
     2521 cttttaatta ccctaatagc aggtggcggt ataataggca ctattattgc tttgaagatc
     2581 aacatgacgg ccataccaca actggttgct gcttttcatt ctttagtagg tatggcagca
     2641 gttctggtgg cctattgtgc ctttttatcc cccgcgtcct ttcaaatcgg tacacctggt
     2701 gctattgcta ctgcaagtct aattgaaatg agcttgggat taatcatcgg agccattacc
     2761 ttttctggtt cagttattgc ctttttaaaa ttgcaaggac tcatatcagg tattcccatt
     2821 cgattttatg ctcaaaacta tgtcaactta ctaataggac taacaatcct ggtgttactc
     2881 attttatttg tagccaatca aaacctgttt ctctttaacc tgctcgcggg actagctttt
     2941 gtcattgggg ttttactgat tattcctatt ggcggagctg atatgcctgt cgttatttcc
     3001 atgctgaact cctactcagg ttgggccgca gcaggaatag gtttcactct gagtaatcat
     3061 ctgttagtta tcacaggcgc tctggtaggc gccagcgggg caattttaag ctatatcatg
     3121 tgcgttggta tgaatcgctc tatttttaat gtcatttttg gtggaataaa cagttctgga
     3181 aatacgaaac aagctcattc tgctaatgag ttacgcaatg tcagacaagc caacggcgaa
     3241 gatgctgcat tcctattaag taatgccaag gatgtcatca ttgttcctgg atatggaatg
     3301 gcagtggcac atgctcaaca tgcggtgaaa gagcttgttg atgccttgga atctcgatct
     3361 attcgtgttc gttttgcaat acaccctgtt gccggacgca tgcctggcca tatgaacgta
     3421 ttacttgctg aagcaaatat cccctatgac agagtctttg agcaaagtga aattaacagg
     3481 gattttgcta cctgtgacgt tgcttacgtg attggagcta acgatatcac caaccctgca
     3541 gctaaaactg atccttcctc ccccatttat ggtatgcctg tcctggaggt tgaaaaagct
     3601 aaaacggtct tattcgtcaa acgcagttta tctcctggtt atgccggtgt ggaaaacgat
     3661 ttattttttc acgacaacac ctacatgctt ttcggtgatg ccaaagtaat gactgaatca
     3721 atagctaagg ctttgggaga aatataaccc tttcaaaata aaagcaataa tctctgacgg
     3781 ggtgggtacc cggttcaatc gacgcaaatg ccttttaatt tgccaacgaa gcaaaataaa
     3841 tgtatcttag cccgttaatt tttcataatg gagatatatc tatgctacga aaaatcggct
     3901 ttacgttgtt atgcgcagtg gcaacattta gttcaagcac ttattctagt gtgactcaca
     3961 cactccaagc tggagtaacg ctggaatacg agttcccccc taatgaacct ctagtctttg
     4021 ttaattattt gtattgggaa gtagcggccg attgtaaaat taccagtgaa gatccatcca
     4081 atgaactttt tgcagaggca ctcgcaaaag ctggtaaaat taatggtgtt acgctctcta
     4141 aaggaaatac catgaaagtg actgtccatt ccggcgatat tttaaaacta agcgcggaat
     4201 caggagcaaa agttcaaatc accaattttg gaccacatac tctcaaagca acttgtactg
     4261 cataaagaat tgagctaacc gcaccccaat actgaacagt aatggggtgt gaacattcaa
     4321 atcctgttca tcaatgttct cgagtagcac agaacttaat cttgggataa cgttcttctg
     4381 ccattgtcaa attcactttg gtaggcgcca ggtacataag catatctcct ccatccaaag
     4441 ccaaataatc ataagccttg gtacgaaatt cactcattgc tttatcatct tcagaataaa
     4501 cccatcgagc gcaagtcaca ttgactgatt cgtaaataca atccacttta tattcatgtt
     4561 tcagtctatg cgctaccaca tcaaattgta atatcccaac agctcccaat attaactgat
     4621 tgctgtttaa tggcctaaaa acctgggtag caccctcttc agataattcg attaatcctt
     4681 taagcaaggc tttactcttt aatgggtccc tcaacctgac aagcctaaac aattcaggag
     4741 caaaattagg aattccagta aactttaatt gttcaccttg tgtaaaagta tcaccaatac
     4801 gaatagtacc atgattatgc aaaccaataa tatcaccagc taaagcaagt tctgtgtgtg
     4861 atctatcgcc tgccataaaa gttaaggcat tggaaatttg cacttctttt cctatgcgta
     4921 aatgacttaa tttcattccc ttcttgtaag atcctgaaca aacccgcaca aaggcaattc
     4981 ggtctctgtg tttgggatcc atattggctt gaattttaaa aacaaaaccg gaaaatgttt
     5041 cttcattcgg agaaacaact ctctcctgag ttgctcgagg ttgaggtcct ggcgcatact
     5101 cgacaaaatc gtccaataat tctttaatac caaaattatt aattgctgaa ccaaagtata
     5161 ccggggtcat ttttcctgca agataatctt ccaagttaaa cccgtgggac gctcctttga
     5221 ctaattcaat ttcttctctg agttcgtctg cactgtcacc tatcaactca tctaattgag
     5281 gattatccaa tcctttaatc tgcatagctt ccactttttt tgcattactg ccttgctgat
     5341 ataaataaat aatatcttga tagcggtgat aaattccctt gaagcgttta cccattccaa
     5401 ctggccaggt aattggcgca cattgaatgc ccaaaacaga ctctacttca tctaacaaat
     5461 caataggttc tcgcccttct ctgtccaatt tattgataaa agtcatgatc ggtgtatcac
     5521 gtaaacggca aacttccatc aattttaccg ttcgctcctc tactcccttg gccacgtcaa
     5581 ttaccattaa ggcagaatct acagcagtga gagtacgata ggtatcttca gaaaagtctt
     5641 catggcctgg tgtatctaac aaattgatga cgtgttgatt atgtataaac tgcataacag
     5701 acgtggtaat cgatattcct ctctcttttt ccatttccat ccaatcagaa gtggcatgtc
     5761 tgtctgcttt acgacctttg accgtacctg ccaactgaat agctcctcca aaaagcaata
     5821 acttttcagt tacagtggtc tttcctgcat caggatgaga aataatggca aaagttcgcc
     5881 ttttattaaa gtcttgataa aaatcagaca taggaatctc ggattaagtg gttaaatgcc
     5941 ttgaattgta aaggaaaagt acaaaactaa caattttttc gttaataatt cgcaagccat
     6001 aagtgtttcg tcaatttgga tcgttattaa tccacaaaat acaattaaaa atctacttaa
     6061 ccattaattg atcaatttgg ggtttattga tataatgcgc gcttttttgt ggagacctat
     6121 atgtcaacat cagaatcaga attaagaaaa tcaatgcaac aatatggaaa gcaggcattc
     6181 agtgaaacag aacaaataaa aattttgaat ttgttacttg aagatgcgaa gaaacagctt
     6241 tccagagaa
//
LOCUS       DBNCWK010000151         6196 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.151, whole genome shotgun sequence.
ACCESSION   DBNCWK010000151 DBNCWK010000000
VERSION     DBNCWK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.151"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..810
                     /gene="lem11"
                     /locus_tag="AC06IO_002554"
     CDS             <1..810
                     /gene="lem11"
                     /locus_tag="AC06IO_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem11"
                     /protein_id="HIO9875722.1"
                     /translation="KISDDLSIYCSDFTYILSDKTILQELKIEGELAAIRKDYLKKKR
                     TNPEVTPFVDQKDVANPFEIYKKIGELAKIDLLKDLENRTLAYLNFSVKIQTEKIADM
                     LNDLLNRVQNQQIIVSELYQYLLIYKGKDDLFDALIFSLKRELKKLSHNFAPKTQPSS
                     ISKINLVLVPSLKLEHLPRHNPEIVNICEKLENYVFRGSNKKSSFFSAKNHCGYTKEV
                     NEIVQRILRGDLLTAEAIFHELAKINIESRKDELILLRNDLQSEKVCVRKL"
     gene            975..2294
                     /locus_tag="AC06IO_002555"
     CDS             975..2294
                     /locus_tag="AC06IO_002555"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIO9875723.1"
                     /translation="MKHQSNLSGRNVYVVDGSRTPFLKAKGVGPFCGSDLAVAAGTTL
                     LNRQPFSPDELDEVIIGSAMPGPDEANIARVVALRLGCGDKVPAFTVMRNCASGMQAL
                     DNAAIQIATGRSNLVLAGGTDAMSHAPLLFNEKMASWLANWFAAKSMGQKLALITKFK
                     PSYLAPVIALLRGLTDPIVGLNMGQTAEKVAYRFNITREQMDEFAAQSHLRIAQAYAE
                     NRMSEVAPIIDYKGCIYPQDDGIRADSTVEKLSKLKPFFDKKYGMVTPGNSSQITDGA
                     CLLLLASAEAVKKYALPVLGRIVDSQWAALDPSQMGLGPVHAATPILERHNLKPQDLD
                     CWEINEAFAAQVLGCLAAWNDEEYCKTQLGLKEAIGAPSLERLNRDGGAIALGHPIGA
                     SGARIVLHVLKSLEQKNESRGMAAICIGGGQGGAMYLERVTEVKGHE"
     gene            2287..4305
                     /locus_tag="AC06IO_002556"
     CDS             2287..4305
                     /locus_tag="AC06IO_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIO9875724.1"
                     /translation="MNNYKHWDLQRDKDAILWLGLNRRDAAVNSINEEVLDELNSLLH
                     EISHDKKAMGLIIYSLKEKGFIAGADVNAFSQFESSSQAVDFLQKGQTVFARLQALSI
                     PSVAMIDGFCMGGGYELALACTYRIATDDKDTRIGLPEVMLGIHPGWGGTVRLPQLIG
                     GFKALSQVILTGSAIPAVKAKSLGMIDEVVPIRQLRRAAVYFIKNKPPRHRASFIESL
                     TNSSLLRKAIAYLLRYNVAKRVRKEHYPAPYAVIDLWEKEGGVGERAYLKEVDSIEKL
                     VSQNDTAKNLIRAFLLRERLKGFAKDSHFKTAHVHVIGAGVMGGDIAAWCALRGLQVT
                     LQDKSYEQIAPAIGRAHKLYSKKLRKPRLIQAAMDRLIADPHGHGVKRADVIIEAVFE
                     NLAVKQEIMKTVEKNAKKDAIIATNTSSIPLDEISSVMSKPERLVGIHFFNPVAKMDL
                     VEIVSSSSTTKKVQQSACAFVNQIGKLPLPVKSSPGFLINRVLMPYLMECVQLLDEGY
                     SAETIDEAALQFGMFMGPVELADTVGMDVCLAVAENLTGHFGGTIPQRLRDLVKEGKL
                     GRKTGEGFYKYKNGKVIKKKAPSHVDKTIADRLILRMVNESAACIREKVVEDSDLLDA
                     GMIFATGFAPFRGGPMNYANDFGKDKLETLFKTFESKYGERFKVDTSL"
     gene            4365..5090
                     /locus_tag="AC06IO_002557"
     CDS             4365..5090
                     /locus_tag="AC06IO_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIO9875725.1"
                     /translation="MEQKSKSIIRVWYLILLLSQYSFANVYVLPEAGNTIGEIQVAFS
                     EYGETIDEVGRRFNVGYYEMVRANPGVNARYPLTANTKLIIPSRFILPNVPRKGVVIN
                     LAEYRLYYFPPDDNVVMTYPVGIGKKGWETPLGLSRIISKEINPTWRPTVKLQKAAES
                     IGAPIPSEFPPGPHNPLGQHVLRLGWPAILIHGSNRVDGIGAKVSAGCIRMLPEDIEH
                     LYSLVSVGTPVRIINAPLQRQAD"
     gene            complement(5151..6026)
                     /locus_tag="AC06IO_002558"
     CDS             complement(5151..6026)
                     /locus_tag="AC06IO_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIO9875726.1"
                     /translation="MQVIPFRYFILLAGTIVFLSLVALVINHFIYQFPGNNYFPPNTP
                     VMACIILLNYTGLILFFGKKNRVTCSGLELINFFIVMSIIALATNAVQLTPYPPIDKQ
                     IIKLEAQLGINMISILEWTNKYQHFKYLLAIIYDTLPYQMSILPLCVIVSGRFYILKD
                     YYFLLLCTTLVGFSYYYFFPTTAPASELQSSLFAPEQIATGLKFHQIHNHIHPTTIEG
                     GLIALPSFHTIWAILCVYLIKDWFIPCLLLSIINVLLILSCVLLGWHYPIDILAAIIL
                     VGLSYYLLTVIKVEV"
     gene            complement(6037..6195)
                     /locus_tag="AC06IO_002559"
     CDS             complement(6037..6195)
                     /locus_tag="AC06IO_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HIO9875727.1"
                     /translation="MRISNTVVKILAFKTQENGNIKFGVEAPRSINIHREEVFHAIKQ
                     KETLSTAD"
BASE COUNT         2002 a         1033 c         1363 g         1798 t
ORIGIN      
        1 aaaatatcag acgatctatc catttattgt tctgatttta catatatctt atcagataag
       61 accattttac aagaattaaa aattgaaggg gaattagctg cgattagaaa agattattta
      121 aagaaaaaaa gaacaaaccc ggaggtcact ccatttgttg atcaaaaaga tgtagcaaat
      181 cctttcgaaa tatacaaaaa gataggtgaa ttagccaaga tagatctact gaaggatttg
      241 gaaaaccgta cacttgccta tctgaatttc tcagtaaaaa tccagacaga aaaaatagcc
      301 gacatgctaa atgatttatt aaatcgtgta caaaatcagc aaattatagt ctctgaactg
      361 taccaatatc ttctaattta taaaggcaag gatgatttgt tcgatgcctt gatattttca
      421 ctcaaaagag aattaaaaaa attaagtcat aatttcgctc ccaagacaca accttccagc
      481 atatcaaaga taaatctcgt gcttgttcca agtttaaaac ttgagcacct gcccaggcac
      541 aatccagaaa tagtgaatat ttgtgaaaaa ttggaaaact atgtctttag aggatccaat
      601 aagaaaagtt catttttttc agcaaaaaat cattgtggct acaccaaaga ggtaaatgaa
      661 atagtgcaaa gaatattgag gggagatctt ttaacagcag aagcaatttt tcatgaatta
      721 gctaaaatta atatagagag tcgtaaagat gaattgattt tgcttagaaa tgatttgcaa
      781 agtgaaaagg tatgcgtaag aaagctttaa cagaataagg cttgatgtat aaacacctcc
      841 aggaatgatt tacgtaattt aatgtcatca aaaaaatcat cttaagattt ccaaaatatc
      901 aagaatgctg ctaatctttg aatctgttga ttttagtata gattcttatg cagaggccct
      961 aacaaggagg actaatgaaa caccagtcga atttaagtgg tcgtaacgtt tatgttgtcg
     1021 atggaagtcg aacgcctttt cttaaagcaa aaggagtagg tccattctgt ggttcagatt
     1081 tggcagttgc tgcaggaaca actttgctaa atcgtcaacc attttctccg gacgagcttg
     1141 atgaggtcat tattggtagc gctatgccag gaccagacga ggccaatatc gctcgcgtgg
     1201 ttgctctgcg tttgggatgt ggtgataagg tgcctgcatt tactgtaatg agaaattgtg
     1261 cgtcaggcat gcaggcattg gataatgccg caatacaaat tgctacaggg cgcagtaatt
     1321 tggtcctcgc tggtggaaca gatgccatga gccatgcacc gttgttattt aatgaaaaaa
     1381 tggcttcctg gctggcaaac tggtttgcag ctaaaagtat ggggcaaaag ctggctttaa
     1441 ttacaaagtt taaaccttct tatctggcac cggtaatcgc tttgctcaga ggcctgactg
     1501 atccgatagt aggattgaat atgggacaaa cagctgagaa agtagcttat cgatttaaca
     1561 tcacaagaga gcaaatggat gaattcgcag ctcaaagcca tttaagaata gctcaagctt
     1621 atgctgaaaa tagaatgtct gaagttgccc caattataga ttataaaggt tgtatatatc
     1681 ctcaagatga tgggattagg gcagattcta cggtggaaaa attgtcaaaa ctaaaacctt
     1741 tttttgacaa gaaatatggc atggtcaccc caggtaatag ttcacagata actgatggtg
     1801 cctgtttatt gctattggcc agcgctgagg ctgtaaaaaa atatgcttta ccggtattag
     1861 gtcgaatagt tgattcacaa tgggctgctc tagatccctc tcaaatgggt ctgggtcctg
     1921 ttcacgcggc aacccctata ttagaaagac ataatttaaa gccacaagat ttggattgtt
     1981 gggaaatcaa tgaggccttt gccgcccaag tcttagggtg tttggctgca tggaacgatg
     2041 aagaatactg caaaacacag cttggcctta aggaagcaat tggggcgcca tcgctggagc
     2101 ggctcaatcg ggatggagga gcaattgcgt taggtcatcc tattggggcc agtggggctc
     2161 gtattgtatt gcacgttctt aaatctttag agcaaaaaaa tgagtcgcga ggtatggcag
     2221 caatttgtat cggaggtggt cagggaggag ctatgtatct tgaacgagtt actgaggtga
     2281 aaggacatga ataattataa acattgggat ttacaacgag ataaagacgc cattctgtgg
     2341 cttggattaa atagaaggga tgctgctgtt aatagcatta atgaagaagt cctggatgag
     2401 ttaaacagct tgctgcatga aatatcgcat gataaaaaag caatgggatt aattatttat
     2461 tcgctcaagg aaaaagggtt tattgcgggt gctgatgtta acgctttttc acaatttgag
     2521 agttcttcac aagcggttga ttttttgcag aaaggacaaa ctgtatttgc tcggttgcaa
     2581 gctttatcaa ttccaagtgt cgcaatgatc gatggttttt gcatgggcgg tggttatgag
     2641 ttagctttag cctgcaccta ccgaatcgca acggatgaca aagacacacg tattggtttg
     2701 cctgaagtca tgctggggat tcatccaggg tggggcggta ctgtgcgttt gcctcagtta
     2761 ataggtggtt ttaaagcttt atcacaagtc atattaactg gtagtgcaat acctgctgta
     2821 aaagctaaaa gcctcggtat gattgatgag gtagtaccta ttcgtcagct aagaagggct
     2881 gctgtttatt tcataaagaa taaaccacca cgacacagag catcatttat tgaaagttta
     2941 acgaatagct ctctgcttag aaaagctatc gcttatttac tgcgttataa tgtagccaag
     3001 cgagttcgaa aagaacatta tcctgcacca tatgccgtga ttgacctgtg ggaaaaagaa
     3061 gggggagtgg gagagagggc ttatcttaaa gaagtggact ctatagaaaa acttgtatcc
     3121 caaaatgata ccgccaagaa tcttatcaga gcatttttat tgcgtgagcg attaaaaggg
     3181 tttgccaaag acagccattt caaaacggca catgttcatg tgattggagc aggggtgatg
     3241 ggaggggata tcgctgcctg gtgtgctttg cgaggtcttc aggtcacatt acaggataag
     3301 tcttatgaac aaattgcccc tgcaataggg agggcacata aactttatag caaaaaactg
     3361 cgtaagcctc gattgatcca ggctgcaatg gatagattaa ttgcagatcc ccatgggcat
     3421 ggggtaaaac gggcagatgt cattattgag gctgttttcg agaatttggc cgttaaacag
     3481 gaaataatga aaactgtgga aaaaaatgcc aaaaaagatg cgatcatagc gacaaacacc
     3541 tctagtatcc cacttgatga aatcagcagt gtaatgagta aaccagaacg cttggtaggc
     3601 attcatttct ttaatcctgt agctaaaatg gatttggtag aaatagtcag cagctcatct
     3661 actacaaaaa aagttcagca gagcgcttgc gcgttcgtca atcaaattgg caaattgcct
     3721 ttgcctgtca aatcaagtcc tggattttta ataaatagag tgctcatgcc ttacctgatg
     3781 gagtgcgtac agttattgga tgaaggatat agtgcggaga caatagatga ggccgcatta
     3841 cagtttggta tgtttatggg ccctgttgaa ctagctgata cagtaggaat ggatgtttgt
     3901 cttgctgtgg ccgaaaatct gaccggtcac tttggtggta ctattccaca aaggttacgt
     3961 gatttagtaa aagaaggtaa gctaggcaga aaaactggag aaggttttta taaatataaa
     4021 aatggaaaag taattaagaa aaaggctccg tcacatgtcg ataaaaccat tgcagacaga
     4081 ttaattttgc gcatggtgaa tgaatctgca gcttgtatac gtgagaaagt ggttgaagat
     4141 tctgacttgt tggatgctgg aatgatcttt gccacaggtt ttgctccatt ccgtggaggg
     4201 cctatgaatt acgcaaatga ttttggtaaa gataaactgg aaactttatt taaaacattt
     4261 gaatctaaat acggcgaacg ttttaaggta gataccagct tataatttct ctgcaaggta
     4321 agacgttatg gttttacctt gctttttaga gagaaaagca tcatatggag caaaaatcaa
     4381 aatctataat aagagtttgg tatttaatac ttttactcag tcaatattct tttgcgaatg
     4441 tttatgtatt gcccgaagcg ggaaatacca tcggtgaaat tcaggtcgca ttttcagagt
     4501 atggagaaac tatagatgag gttggccggc gttttaatgt cggttattat gaaatggtac
     4561 gtgctaaccc gggagtgaat gccagatatc cgctaaccgc taacacaaaa ttaattattc
     4621 catctcgttt tatcctcccc aacgttccca gaaaaggtgt agtgattaat ttagcagaat
     4681 atcgattata ttattttcct ccagatgaca atgttgtcat gacttatcct gttgggattg
     4741 gtaaaaaagg atgggagact cctttgggat taagcagaat catctccaag gaaattaatc
     4801 ctacctggag acctacagta aaattacaaa aggcagctga aagcattggt gctcctattc
     4861 ccagtgagtt cccccctggt cctcacaatc ctttaggtca acatgtattg cgtttagggt
     4921 ggccagctat cttaatacat ggttctaatc gagttgatgg gattggggcc aaagtcagtg
     4981 cagggtgtat tcgtatgctt cctgaggata ttgagcatct atatagttta gtgtctgtag
     5041 ggacgccagt aagaattata aatgctcctc tacaaaggca agctgattga aagcaataac
     5101 tattttcagg tatgggtttt cattcagatt aatgcctata aatccaaata tcagacctca
     5161 actttaatta cagttaacag ataataagat aatccaacaa gtattatggc cgccaaaatg
     5221 tcaatcgggt aatgccatcc cagcagtaca catgaaagga ttaatagaac attaatgatt
     5281 gacagcaata agcagggtat aaaccagtct ttgattaggt agacacacaa aattgcccag
     5341 attgtatgaa atgagggaag agctattaaa ccaccctcaa tagttgtggg gtgtatatga
     5401 ttatgaattt gatgaaattt aagtcctgta gctatttgtt caggggcaaa taaactgctt
     5461 tgtagttcac ttgcaggtgc tgtagttggg aaaaagtagt aataagaaaa ccctactaat
     5521 gttgtacaga gtaataggaa ataataatct ttaaggatat aaaatcgccc tgatacaatt
     5581 acgcaaaggg gtaagatact catttgatat ggtagtgtat cgtagataat cgccaataag
     5641 tatttaaaat gttgatattt attcgtccac tccagaatag atatcatatt aatacccagt
     5701 tgcgcctcca gtttaataat ttgtttgtct ataggaggat agggagttaa ttgaacggcg
     5761 tttgtagcta aagcaatgat tgacatcaca ataaaaaaat tgatcaactc taatccagag
     5821 caagtcactc tattcttttt gccaaaaaat aaaatcaacc cagtataatt taacaagata
     5881 atgcatgcca tgactggggt attaggtgga aaataattat tcccggggaa ttgatagata
     5941 aaatgattga taactaaagc aactagcgat agaaatacta tagttccagc caataaaata
     6001 aagtatctga aaggtataac ttgcatttat ttgttattag tcagctgtgc taagagtttc
     6061 tttttgttta attgcatgga aaacctcttc tctgtgaata ttgattgacc tgggtgcctc
     6121 aactccaaat tttatatttc cattttcctg tgttttaaaa gccaaaattt taacaacagt
     6181 attcgagata cgaatg
//
LOCUS       DBNCWK010000152         6121 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.152, whole genome shotgun sequence.
ACCESSION   DBNCWK010000152 DBNCWK010000000
VERSION     DBNCWK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.152"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            33..851
                     /gene="phhA"
                     /locus_tag="AC06IO_002560"
     CDS             33..851
                     /gene="phhA"
                     /locus_tag="AC06IO_002560"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HIO9875728.1"
                     /translation="MEFSSRYVAHVPDAQGLVDYSAQENRIWNILFERQLKLLPGRAC
                     DEFLSGLQTLGLNSSTIPQLPEVSERLKAKTGWQVAPVAALISAREFFELLAEKYFPA
                     ATFIRSEEELDYVQEPDIFHELFGHCPMLTDRVYAEFVHDYACKVLTFPEQDWPLLQR
                     MFWFTVEFGLIKTPKGLRAYGGGILSSISETVYCVESDIPVRILFDPVVAFRMPYRID
                     QLQPVYFVIDSYQNLYDFVLSDMGKFMDRARELGELPPYFDVDPDNPNIHIRAC"
     gene            976..1635
                     /gene="letA"
                     /locus_tag="AC06IO_002561"
     CDS             976..1635
                     /gene="letA"
                     /locus_tag="AC06IO_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator LetA"
                     /protein_id="HIO9875729.1"
                     /translation="MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVK
                     THSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYL
                     TKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMI
                     TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQD
                     D"
     gene            1647..3503
                     /gene="uvrC"
                     /locus_tag="AC06IO_002562"
     CDS             1647..3503
                     /gene="uvrC"
                     /locus_tag="AC06IO_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIO9875730.1"
                     /translation="MNDLQLSAELALFLTKLPSEPGIYRMLDEEGTVLYVGKAANLKK
                     RVNSYFSKQNTGVKTRALVSQIKSIEISVTRSETEALLLESNLIKALRPKYNVLLRDD
                     KSYPYIHLSNHPDFPRVELYRSKKKPPSGNFFGPYPGVAAVRETIVTIQKIFKIRNCR
                     DSYFKARSRPCLQYQIKRCTAPCVHYISPENYKLSVEDAIRFLQGKCQIILDELAERM
                     KQAVSQLNFEEAAVLRDQIKNLRLIQEQQGVVQLRGDADVIAIEVRPGFACIQCVTIR
                     EGQVLNSQSFFPTVPYAVLDEELDANSLWQQTFEAFIGFYYLDTSERIPDLIITNQSI
                     TESRSLEYILSQRRSKSCKIQVNPRGVKSRWMDFAVNNLRISVAEYVSKHSTIRSRYQ
                     ALKQLLALDKNIERMECFDISHTQGEATVASCVVFDTEGPRPSEYRRFNIEGITPGDD
                     YAAMEQAVTRRFKRLIGAQLLPDILIIDGGKGQVSIVKRVLTSLGVEDITLLGVSKGP
                     SRKAGWEKLILVNENREFVLPEDSKALHLLQHIRDEAHRFAITAHRKKRQKTRVESTL
                     ESIEGVGAKRRQALLQRFGGLRELAKAPLEEICKVQGISEQLAKRIYEHFHP"
     gene            complement(3527..4801)
                     /gene="lcl"
                     /locus_tag="AC06IO_002563"
     CDS             complement(3527..4801)
                     /gene="lcl"
                     /locus_tag="AC06IO_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like adhesin Lcl"
                     /protein_id="HIO9875731.1"
                     /translation="MIHRNKVLNSILLILFTSQIAFAKSNPASQAYVDGKVSELKNEL
                     TNKINSIPSGPQGPRGDKGEAGPKGDRGEAGPQGLPGPKGDRGEAGPQGLPGPKGDRG
                     EAGPQGLPGPKGDRGEAGPQGLPGPKGDRGEAGPQGLPGPQGLPGPKGDKGEAGPQGL
                     PGPKGDKGEAGPQGLPGPKGDKGEAGAVGPQGMPGPKGDKGEAGPQGLPGPKGDRGEA
                     GPQGLPGPKGDRGEAGPQGLPGPKGDKGETGAVGPQGMPGPKGEAGDDGQGVPAGGET
                     GQVLAKSNDLDFNTMWVDPANSGIRRQLGDKALGGTVIYVNALGTHGLVVANSDQVNS
                     NTWWDAQDSITNPAHFDNEGKLYSDWRLPTRFELNLIYMMRNELGNFLAGNYWSSIEK
                     SSANSWVFNSKTGEIKDIAKSKTAAVRAVRAF"
     gene            complement(4969..5628)
                     /locus_tag="AC06IO_002564"
     CDS             complement(4969..5628)
                     /locus_tag="AC06IO_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875732.1"
                     /translation="MPFCFSNQPNILNNKLILKIVVLILFFSNSSFAKTINYPWTVSA
                     SLGYVNYQYVHEGDHITATGRFAIGRDLLMKGPFLFGLELGLQSGNQMRLQMTEEEVD
                     ALGGLLVQTTLKPMVDALATIRITPEFTAPLFLQLKAGTAYRQWQMDRDTINDISQLA
                     GEIMAGCGYAITPNASITLSYQGLFGDKPNFTIDLESGIGHVQKIPVQHAILLGIVLM
                     I"
     gene            complement(5769..>6121)
                     /locus_tag="AC06IO_002565"
     CDS             complement(5769..>6121)
                     /locus_tag="AC06IO_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIO9875733.1"
                     /translation="SKKLKQIHHIGINISLDNFCCPQISLPELLSLPLDYLKVDQRLL
                     IGIEGKIKLRKLLSGIINLTKDLNIKIIQKGIETAEQHEVIKSLGCQYAQGYFYCKPV
                     ALNDLLPLIKKTIH"
BASE COUNT         1702 a         1180 c         1303 g         1936 t
ORIGIN      
        1 gatttttgaa tgagatgagt gaaggagaga ttatggagtt tagtagccgg tatgtcgcac
       61 atgtccctga tgctcagggt ttagtcgatt attcggcaca ggaaaataga atttggaata
      121 ttttatttga gagacaactt aagttattgc caggaagagc ttgtgatgaa tttctgtctg
      181 gattacagac tttaggactt aactcctcta ctattccaca acttccagaa gtaagtgagc
      241 gattaaaggc caaaacggga tggcaagtag cgccagttgc tgctttaatt tcagccaggg
      301 agttttttga attattagca gaaaaatatt ttcctgcggc gacttttatt cgaagtgaag
      361 aagaattgga ttatgttcaa gaacctgata tttttcatga gctttttggt cattgtccta
      421 tgttaactga tagggtctat gctgaatttg tccatgatta cgcatgtaag gtattaactt
      481 ttcctgaaca ggattggcct ttattgcaaa gaatgttttg gttcactgta gagtttggat
      541 tgattaaaac gcctaaaggg cttagagcat acggcggggg aattttatct tctattagtg
      601 aaacggtata ttgtgtggaa agtgatattc ctgtgcgaat tttatttgat ccagtagtgg
      661 cttttcgaat gccttatcgg attgaccagc tacaacctgt ttatttcgtt attgacagct
      721 atcaaaattt atatgacttc gtgctttctg acatgggtaa attcatggat cgtgcgcgag
      781 agttgggtga acttccaccg tattttgatg tggatccgga taatccaaat attcatataa
      841 gggcttgtta gtttgttatt ttgtaagaca gctggacttg tggattagaa tgactatgta
      901 ttgtaatgat tatcgagtct tgttaacatc gaccaacaca gtttgttcat tgactgattg
      961 gtcaaggagc attatttgat taaagtatta attgttgatg accatgcatt ggttagaatg
     1021 ggcattcgac gattgcttga agatatgtcg gatgttgatg tggttgcaga tgctgaaagt
     1081 ggtgagcagg ctttggccta tgtgaaaacc cactcccctg atgtcgtatt attagacatg
     1141 aaaatgcctg ggatagatgg gtgggaagtg acacgtcgct taaaaaaaac aaacccaaat
     1201 attaaagtca tagctgttac agctatttgc tcagatcctc tccctactcg agtattgcaa
     1261 ttaggcgcta tgggatacct tactaaagaa tcaggggcag aggaaatggc tgccgcaatt
     1321 cgtaaagtgg ccaaaggtga gaaatatctt agtgccgaga tagcacaaaa aatggcaatt
     1381 aatagtctcc aggagtctca ggattccccc tttgatttat tgtccgaaag agaaatgcaa
     1441 gtcatgttga tgattactag cggcatgaat gtacaagaca ttgctgatag attattttta
     1501 agtagtaaaa ccatcaatgg ctatcgatat aggatgtttg aaaaattggg gattaaaaat
     1561 gatgtggagc ttacttattt ggctatgaag caccgcatta ttgaacaccc caatgatttg
     1621 tcacaagatg attagatgtt aatttcatga atgacttaca gctttccgct gaactcgctt
     1681 tatttttaac gaaactccca agtgaaccgg gtatataccg catgttagac gaagaaggca
     1741 ccgttctata tgtgggaaaa gcagccaatc taaaaaaaag agtcaatagc tattttagta
     1801 aacaaaatac aggagttaag acacgagcat tggtaagcca aatcaaatcc atagaaattt
     1861 ctgtgacaag atcggagaca gaggccttat tattagagag taatttaatt aaagcgttaa
     1921 ggccaaaata taatgttttg ttgcgcgatg acaaatccta tccttatatt catctatcaa
     1981 atcatccaga ctttccaaga gttgagttat accgaagtaa aaagaagcct ccctccggta
     2041 atttttttgg cccctatcct ggtgtagctg ctgtaagaga aacgatagtc accattcaaa
     2101 aaatatttaa aattcgcaat tgcagagaca gctattttaa agcccgttct cgcccttgtt
     2161 tgcaatatca aataaaacgc tgtactgcgc cttgcgttca ttatatttct cctgaaaact
     2221 acaaattgtc tgtagaggat gccataagat ttttacaggg gaagtgccaa ataattcttg
     2281 atgaacttgc cgaacgaatg aaacaagcgg taagtcaatt aaatttcgaa gaagctgctg
     2341 ttttgcgtga tcagataaaa aatttgcgtt taattcaaga acaacaagga gttgtccagt
     2401 tacgtggtga tgctgatgtg atcgcaatag aagtacgtcc tggtttcgca tgtattcagt
     2461 gtgtgactat tcgtgaagga caagtactga atagtcaaag ctttttccct acagtgcctt
     2521 atgctgtatt agatgaagaa ttggatgcca attcactttg gcagcagact tttgaagcct
     2581 ttattgggtt ttattatttg gatacatcag aaagaattcc ggatttgata attactaatc
     2641 aatcaataac agagagtcgc tcattagagt atattttgtc ccagcgacga agcaaatcct
     2701 gtaaaattca agttaatcca agaggtgtca aatcgagatg gatggatttt gcagtgaaca
     2761 atttacgaat ttcagtagca gaatatgtca gcaaacattc tacgataaga tcaagatatc
     2821 aggcattaaa gcaattgctt gcgcttgata aaaatataga gcgaatggaa tgttttgata
     2881 ttagccatac tcaaggtgaa gcaactgttg catcttgtgt tgtatttgat acagaaggcc
     2941 ctcgccccag tgaatacagg cgatttaaca ttgagggtat tactccaggg gatgattacg
     3001 cagcaatgga acaggcagtt actcgccgat ttaaaagact gattggtgct caattattgc
     3061 cagatatttt gattattgat ggaggtaaag gccaggtttc tattgtaaaa cgagttttaa
     3121 cctctctggg tgtggaagat attactttat taggtgtatc caaggggcct tcacgaaagg
     3181 cgggatggga gaaattgata ctggttaatg aaaacagaga atttgttttg ccagaggact
     3241 ccaaagcgct tcatctttta caacatattc gtgatgaagc gcatcgtttt gctattactg
     3301 cccatcggaa gaaaagacaa aagacacgtg ttgaatccac tcttgaaagt atagagggtg
     3361 ttggcgcgaa acgacgtcaa gcgttgctgc agcgttttgg cgggttgcga gagttggcta
     3421 aagcgccact ggaagaaatt tgtaaagttc agggaatcag cgagcagttg gcaaagcgaa
     3481 tttatgaaca ttttcatcct tgagcaaaat tgaagttttg ccatagttaa aaggctctta
     3541 cagcacgtac agcagccgtt ttacttttgg caatgtcttt aatttcccct gttttagaat
     3601 taaataccca gctgtttgcc gatgattttt caatcgaact ccagtaatta cctgccaaaa
     3661 aatttcccag ctcatttcgc atcatataaa ttaaattcaa ttcaaaacgg gtagggagcc
     3721 tccagtcaga atataattta ccttcattgt caaagtgggc aggatttgtt atggaatctt
     3781 gagcatccca ccatgtgttt gaattaactt gatctgaatt tgctactaca agcccatggg
     3841 tacctagcgc attaacataa atcactgtac cgccaagagc tttatcgcct aattgccgcc
     3901 taatgccgga atttgccggg tcaacccaca tggtattgaa atcgaggtca tttgatttgg
     3961 caaggacttg tcccgtttca ccaccagcag gcacaccttg gccgtcatct cctgcttcgc
     4021 ctttaggtcc tggcatacct tgtggaccca ctgctcctgt ttctcctttg tctcctttcg
     4081 gtccgggtaa tccctgaggt cctgcttccc ctcggtctcc tttagggccg ggtaatccct
     4141 gaggtcctgc ctctcctcgg tctcctttag ggccgggtaa tccctgaggt cctgcctccc
     4201 ctttgtctcc tttaggtcct ggcatacctt gtggacccac tgctcctgcc tcccctttgt
     4261 ctcctttagg gccgggtaat ccctgaggtc ctgcttcccc tttatctcct ttagggccgg
     4321 gtaatccctg aggtcctgcc tcccctttat ctcctttagg gccaggtaat ccctgcggcc
     4381 cgggtaatcc ctgcggtcct gcttcccctc ggtctccttt agggcctggt aatccctgcg
     4441 gtcctgcttc ccctcggtct cctttagggc ctggtaatcc ctgcggtcct gcttcccctc
     4501 ggtctccttt agggcctggt aatccctgtg gccctgcctc ccctcggtct cctttagggc
     4561 ctggtaatcc ctgtggccct gcctcccctc ggtctccttt agggccagcc tcgcctttat
     4621 ctcctctagg tccttgaggt ccagagggga tgctgttgat tttgttggtt aattcatttt
     4681 ttaattcaga taccttgccg tccacatagg cttgcgaggc cggattgctt ttggcaaatg
     4741 ctatctgtga tgtgaataag attaataaaa tgctattaag gactttattt cgatgtatca
     4801 ttctttatat cccactgtat gctcacttgt ctcttttatt ttaccgctgc gttgcgagcg
     4861 tttctgcggt gctcatttac tgcttgtaaa tcctactcct caaaacgttt gtaccttgcc
     4921 ctaaaacaaa ataagcaggt tatgcaagag atctattgga aaaattagtt aaatcattaa
     4981 aacaatacct aacaaaatgg cgtgttgaac aggaatcttt tgtacatggc caatgccgct
     5041 ttccagatca atagtgaaat ttggtttgtc tccaaatagg ccttgatacg atagggtaat
     5101 actggcattt ggtgtaattg cataaccaca tccagccatt atttcgcctg caagttgaga
     5161 gatgtcattg atcgtgtctc tatccatttg ccattgcctg taggcagttc ctgctttcag
     5221 ttgtagaaac aaaggggcgg taaattcagg ggtaatacgg attgtagcta atgcatcaac
     5281 cataggtttc aaggtggttt gtactaaaag tcctccgagg gcatcgacct cttcttccgt
     5341 catttgcagt cgcatttgat tcccactttg taaacctaat tcaagtccaa ataaaaaggg
     5401 gcctttcatc aacaaatctc ttcctattgc aaaccgacct gttgcggtaa tatggtcgcc
     5461 ttcgtgtacg tattgataat tgacataccc caagcttgcg gagactgtcc atggataatt
     5521 gattgttttc gcaaagctcg aattactaaa gaagagaata agcacaacta tttttagaat
     5581 aagtttgtta tttaaaatat ttggttggtt ggaaaaacaa aaaggcataa gctaacttcc
     5641 attgcaatgt atgattctta tatcaattaa ttatgacgaa aaactgtcta agtaatggtt
     5701 caaaacattt agaatcttat aagattattg cgtttgatgc cataaaatta ggttgaatta
     5761 ttaattactt agtggatagt tttttttata agtggcagta aatcatttaa ggcaacaggt
     5821 ttgcagtaaa aatatccttg tgcgtattgg catcctaaag atttaatcac ttcatgctgt
     5881 tctgcagttt caataccctt ttgaatgatt ttaatattaa gatctttagt taaattgatt
     5941 attcctgata ataatttacg taatttgatt tttccctcta tgcctattaa taatcgttga
     6001 tctactttga gataatcaag tggtaatgac agcaattcag ggagggaaat ttgtgggcaa
     6061 caaaaattat ccaagcttat attaattcca atatgatgta tttgttttaa tttttttgat
     6121 a
//
LOCUS       DBNCWK010000153         6032 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.153, whole genome shotgun sequence.
ACCESSION   DBNCWK010000153 DBNCWK010000000
VERSION     DBNCWK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.153"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            118..456
                     /gene="rpsF"
                     /locus_tag="AC06IO_002566"
     CDS             118..456
                     /gene="rpsF"
                     /locus_tag="AC06IO_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIO9875734.1"
                     /translation="MRHYEIMFLVHPDQSEQVPGMVERYEGIITKHNGKIHRKEDLGR
                     RQLAYSINKVHKAHYILMNVECNLDALNEIKNAFKFNDAILRHLITVQKQAITTESVL
                     MKKEKETKVA"
     gene            470..697
                     /gene="rpsR"
                     /locus_tag="AC06IO_002567"
     CDS             470..697
                     /gene="rpsR"
                     /locus_tag="AC06IO_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIO9875735.1"
                     /translation="MSAYFRRKKMCRFSAEGGNEIDYKDINLLKNYITETGKIVPSRI
                     TGTQTRFQRQLAKAIKHARFIGLLPYCDSHR"
     gene            651..1610
                     /locus_tag="AC06IO_002568"
     CDS             651..1610
                     /locus_tag="AC06IO_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875736.1"
                     /translation="MPDLLVYCLIATAIDKMNFAGNFILRQSKATLENKQYAIVCAVV
                     LSIVPFASWLSVALMSLITLRKGEKPGFEIMLPAMIVHSVPLMMFLPLEGALINTLVA
                     YLPCYIAALTLRKTASWSNVFGAFLIQAFLVFLSIEILIPDFVSDQFNHFKVLLSQFQ
                     DYKQLVESSTDGLNNLSLAQLFFGIQILSVIVSALISLMFARTIQAKLFMPGGFKQEI
                     LEFRSGRFSFLMLIIVSVASYFEVPVAISTLPLLLAYFFLSGFSLAYYILARKWQVRV
                     VVLLFLLILLKPFFVLFAYIVFGSLDSLFNFRLYLPARVREST"
     gene            1623..2072
                     /gene="rplI"
                     /locus_tag="AC06IO_002569"
     CDS             1623..2072
                     /gene="rplI"
                     /locus_tag="AC06IO_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIO9875737.1"
                     /translation="MEVILLEKVRNLGNLGDKVHVKSGYGRNYLIPQNKAVFATEQNI
                     ELFEKRRAELEKKAQQDLANAEQRAAKLNDTTIVISAMASDEGKLYGSVGVNEIKDAL
                     IEKQIEISKREIVMPEGPLHSIGNYVVEVHVHSDVVANLQVEIIPAK"
     gene            2281..4299
                     /gene="legC6"
                     /locus_tag="AC06IO_002570"
     CDS             2281..4299
                     /gene="legC6"
                     /locus_tag="AC06IO_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC6"
                     /protein_id="HIO9875738.1"
                     /translation="MSHSKREQNPIARNLSDFRQVATLLNNEGLVVTEHNYKKVQALL
                     NRYKELATFNDQNQSKLNGKQIGELNQIRLIISPEERREIRRSVTRIENGIKNREERI
                     KEEYMRIEKQQAKAREAVQVLWPQLQKAAKKFINSGIANMEELLKEYKDLRNELYNHA
                     LNLPTQERKQKNQEMAELSRLVNIHESKLYEEINRARKVIEAAVTQAQKNVDSMSLLQ
                     MVRLMLPKLKDADILKIAQKAGVLSLQDTTTSEHGATLDKLVVREQPLEKQSALISIM
                     FKELALNGAQAGPLAKYLQAVQENAKDLISGKKTHFTLLYQTNEGEIGGFYFNIHELL
                     QNQHQAISKLVHEMYKRGVDQNSRVVMAFASPEQAASLMLANGENIKALTEAGNLQEL
                     TEQLLENAGDSKALARIVGHDVVIGQKTSEIIRDLNRSKEQNGIEPAEVMKLFQIIAV
                     IFETRNILINWINAPVHLKEQTEEALLGAGKTPAIVEGKGTQPKRVRFLPDLVEKLKA
                     TSTDTEQPVTKRTDDELLDKKIQVALEKVDQALEELKGKIGDINQHKYDKAYEAAKIL
                     LRQLQETRDQYAIDLANPQINFKEAGLKFKEASNLVIQKAKPILEKDLGWGEYLKNLF
                     KVIVNAIVFCVTFGASQGFFATTRAKSAEAVEKAESELELNQLGSLPK"
     gene            complement(4397..4954)
                     /locus_tag="AC06IO_002571"
     CDS             complement(4397..4954)
                     /locus_tag="AC06IO_002571"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIO9875739.1"
                     /translation="MARTLSNMLPLGTVAPHFELLDVVSGSKVKLRETPRYVATVIMF
                     ICNHCPFVKHINNELTQLANDYIPQGIRFLAINSNDVESYPDDSPENMIQTAKVHQYP
                     FPYLFDETQEVAKAYNAACTPDFFVFDSDLSLVYRGQLDDSRPGNGIPVTGNSIRQAL
                     DCLLNNKPFDFEQKPSIGCNIKWKI"
     gene            complement(4978..5478)
                     /locus_tag="AC06IO_002572"
     CDS             complement(4978..5478)
                     /locus_tag="AC06IO_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875740.1"
                     /translation="MSKTKYDTLYLVVGIVGALLLIYGLSQNNPQVYYITGSLALMIT
                     AIHYKLIYFIALELILGAGHTSLLLGMGRYIGLALPSLLCLQLLIFYLMFGKGNSIFL
                     LTGIIGIALLSMGLAYHNQIIFFSGSSGVAAYSYYSAYRGQYICYIWGILNTIFASYA
                     LCQLVF"
     gene            complement(5482..5970)
                     /locus_tag="AC06IO_002573"
     CDS             complement(5482..5970)
                     /locus_tag="AC06IO_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry protein EnhB"
                     /protein_id="HIO9875741.1"
                     /translation="MSLINYFTKTLLITAFATNAVQAASIFPRGCEVTGFGYSENYLI
                     LNETGNQTYYLIQNRSDSKIELERFDNSDAFMIPPLQASLDPMSWAAFASDVKNLNFK
                     CYKHVDENTALVDCRDVLEVCQYPRVKFALSNMGNYWISTNKSQNEVIQESVAKGIYL
                     KW"
BASE COUNT         2145 a          965 c         1112 g         1810 t
ORIGIN      
        1 attttaaatc gcaattagat tgtaaggact aaataaaaaa gttataattg gcagcttttt
       61 aaaacccttg ccttaccagt actcaatgct ggaaggcttt atccataagg agaattcatg
      121 agacattatg aaattatgtt tctggtacat ccagatcaaa gcgaacaagt acctggtatg
      181 gttgagcgct atgaagggat tattaccaaa cataacggca aaatacatcg taaagaagat
      241 ttaggtcgac gccagctggc atattcaata aataaggttc ataaagcaca ttatatctta
      301 atgaacgtag aatgtaatct tgacgcactt aatgaaatca agaatgcatt caagtttaat
      361 gatgctattt taagacactt aatcactgtt caaaaacaag cgattactac agaatctgtc
      421 ttaatgaaga aagaaaaaga aactaaagtc gcttaagagg agaacatcta tgtcagctta
      481 ttttcgtaga aaaaaaatgt gccgctttag tgctgaaggt ggtaatgaaa tagattataa
      541 agacatcaat ctattaaaaa attatattac tgaaactggt aaaattgtac caagccgtat
      601 tactggtact caaactcgtt tccaaagaca attagccaag gcaattaaac atgccagatt
      661 tattggttta ttgccttatt gcgacagcca tagataaaat gaattttgca ggtaatttta
      721 tactcagaca aagtaaagca actcttgaaa acaaacaata tgctattgtt tgtgcggttg
      781 ttctttcaat agtcccattc gcctcttggc tatcggtggc tttaatgagt ttaattactt
      841 tgagaaaagg tgaaaaacct ggatttgaaa tcatgttacc tgcgatgatt gttcattctg
      901 tgcctttaat gatgttttta cctctggaag gggcattgat caatacattg gttgcttatt
      961 tgccttgcta tatcgctgct ttgactttac gcaaaacagc cagctggtca aatgtatttg
     1021 gagcttttct aattcaagca ttccttgtat ttctgtccat tgaaatattg atacctgact
     1081 tcgtaagtga tcaatttaat cattttaaag tgctattgtc tcaatttcag gattacaaac
     1141 agttggtaga aagcagtaca gatggattga ataatttaag tttggctcag ttattttttg
     1201 gtattcagat attaagtgtg attgtttcag ccttaatctc actaatgttt gccagaacta
     1261 tacaggctaa acttttcatg cccggtggtt ttaaacagga aatattagaa tttcgtagtg
     1321 gcagattttc atttttgatg ttaattattg tgtctgttgc ctcgtatttt gaggtaccag
     1381 tagctattag cactttacca ctgcttcttg catatttctt tttatcgggt ttttctttgg
     1441 cctattatat tttagccaga aagtggcaag ttagagtggt ggttctgtta tttctattga
     1501 tcttgttaaa accattcttt gtcttatttg cctatattgt atttggctcg ctagatagtt
     1561 tatttaattt tcgattatat ctacctgcaa gggtgagaga atcaacgtaa ggggttatta
     1621 agatggaagt aatcttatta gaaaaagtac gtaatctggg aaatctaggt gataaagtac
     1681 acgttaaatc aggttatgga cgtaattatt tgataccaca aaataaggcc gtctttgcaa
     1741 cagaacaaaa tattgaatta tttgaaaaac gtcgtgctga actggagaaa aaagcacaac
     1801 aggatttggc taatgctgaa caacgtgcag ctaaattgaa cgatacgacg atagttatta
     1861 gtgctatggc tagtgacgaa ggcaaattgt atggttccgt tggggttaat gaaattaaag
     1921 acgcattgat agaaaaacaa attgaaatca gtaagcgtga aattgttatg cctgaaggcc
     1981 ctctacattc tataggtaat tatgttgttg aagtacatgt acatagtgat gtagtagcta
     2041 accttcaagt tgaaattatc cctgctaaat aatttgtttg ataccgacct aaaaatattc
     2101 aaatcaacaa tatttatata aattcttact tcaagttgtg tgtcatgacg cacaacttgt
     2161 attctattat tcaatgtgta ttaagatatt gtaattttgc aaaaaattgc ccaattaaat
     2221 gtgacaaaat agcattcgtt gattataatg cgagcacact aaacaccaag gaatcattat
     2281 atgtctcatt ccaaaagaga acaaaaccct attgcccgga atttaagtga ttttaggcaa
     2341 gttgcaacac tattaaataa cgagggtctt gttgttactg aacataacta taaaaaagtc
     2401 caagccttat taaatagata taaagagcta gcaaccttca atgatcaaaa ccaaagcaaa
     2461 ttaaatggaa aacaaatagg tgaattaaat caaattagat taattatttc tccggaagag
     2521 cggcgagaaa tcagaagaag tgttacacgt atcgaaaatg gaataaaaaa tagagaagaa
     2581 agaataaaag aagaatatat gagaatagaa aaacaacagg ctaaggcaag agaagcagtt
     2641 caagttttat ggccacagtt gcaaaaagca gccaaaaaat ttataaattc tggaatagca
     2701 aacatggaag agctattgaa agaatataaa gacttaagaa acgagttata taatcatgcg
     2761 cttaatcttc ctactcagga aagaaagcaa aaaaatcaag agatggctga attatcgcgt
     2821 cttgttaata tacatgaaag caaattatac gaagaaatta atcgagctag gaaggtaatt
     2881 gaagctgcag tgactcaagc acaaaagaat gtagacagca tgtcattgct gcaaatggtt
     2941 cgactcatgc tgcctaaatt gaaagacgca gatatattaa aaattgctca aaaagctgga
     3001 gtactttcct tacaggatac aacaacctct gaacatgggg caactttaga taaattggtc
     3061 gtaagagaac agccattaga aaaacagtct gctctaattt ctataatgtt caaggaattg
     3121 gctctcaatg gtgctcaggc tggtcctctg gcaaaatacc tccaggcagt tcaagaaaat
     3181 gcaaaagatc taataagtgg taaaaaaact cactttactc tgctttatca aacaaatgag
     3241 ggtgaaattg gagggtttta ttttaacatt catgaattgt tgcagaatca gcatcaagca
     3301 atttctaagc ttgtacatga aatgtataaa agaggagtag atcaaaacag tcgagttgta
     3361 atggcttttg ctagcccaga acaagctgcg tctttgatgt tagctaatgg agaaaatatt
     3421 aaagcattaa ctgaagctgg caatttacaa gagttaactg aacaattatt agaaaatgct
     3481 ggtgattcaa aagcattggc tcgcatagta gggcatgatg ttgtaattgg ccagaaaact
     3541 tcagaaataa ttagagatct taatcgttca aaagagcaaa atggtataga gccagctgaa
     3601 gtaatgaaat tatttcaaat aattgcagta atatttgaaa ctcgaaatat tttaatcaat
     3661 tggataaatg ctcctgtgca tttgaaggag caaacggagg aagctttatt gggcgcaggt
     3721 aaaactcctg ccatcgttga gggcaaagga acccaaccca aacgcgtaag attcctacct
     3781 gatttagtgg aaaagctaaa agctacatcg acagatactg aacaacctgt taccaaaaga
     3841 acagatgatg aactacttga taaaaaaata caagttgcat tagaaaaggt tgatcaagca
     3901 ttagaagaac tgaaaggtaa aataggggat atcaatcaac ataaatatga caaagcctat
     3961 gaagcagcaa aaattcttct aagacaattg caagaaacac gtgatcaata tgctattgat
     4021 ttagctaatc ctcaaatcaa tttcaaagag gcagggctta agtttaaaga agctagtaat
     4081 ttggtgattc aaaaggccaa acctatcctg gaaaaggatt taggttgggg agaatacctc
     4141 aagaacttgt ttaaagtgat agtaaacgct attgtatttt gtgtgacttt tggagcaagt
     4201 caaggcttct ttgctacgac acgtgcgaaa tctgctgaag cagtggagaa agcggagagc
     4261 gaactggaac taaatcaact cggttcatta ccaaaataat acatatggtt aactagtttg
     4321 ttatgagagc aacattcgat ttttattata catataatga gtaatggaaa tcgatgttgc
     4381 ttttttgaga ttcagcctat attttccatt tgatgttgca tccaatactt ggtttttgtt
     4441 caaaatcaaa aggtttatta tttaataagc aatccaaagc ctggcgtatt gagttgccag
     4501 ttacaggaat accatttcct ggtcttgaat catctaattg ccctcgataa actaaagaca
     4561 aatcagaatc aaaaacaaag aaatctggtg tgcaagctgc attatatgct tttgctactt
     4621 cctgggtttc atcaaaaaga tatggaaatg gatactgatg aactttagca gtttgaatca
     4681 tattttcagg tgagtcatca gggtaactct ctacatcatt ggagtttata gctaaaaaac
     4741 gaattccttg gggaatatag tcattagcaa gttgtgttaa ttcattattg atatgtttca
     4801 caaatgggca atggttgcat ataaacataa tcacagtagc cacatagcgt ggagtctctc
     4861 ttaatttaac tttgcttcca ctaactacat ccaataattc aaaatgagga gctactgtac
     4921 ctaatggaag catgtttgat agagttctag ccatttaatt aaatccctca aataatctta
     4981 aaataccaat tgacaaagag catatgacgc aaatattgta ttaagaatac cccatatgta
     5041 acagatgtat tgacctctat acgcactata ataagaatat gcagctacgc ctgaacttcc
     5101 agaaaaaaat atgatttgat tatggtaggc aagccccatc gaaagcagtg ctattcctat
     5161 aatgccggtt aataaaaaaa tactgtttcc cttgccaaac attaaataaa aaatcagtag
     5221 ctgtaaacac agtaaagaag gtaaagctaa tccaatatat ctccccatac caaggagtag
     5281 ggaagtatgg cctgccccca aaattaattc cagagcaata aaataaatca atttataatg
     5341 aattgctgta atcatgagag caagtgagcc agtaatgtaa tagacttggg gattattctg
     5401 gctcagccca taaataagta acaaagcacc aacaatacct actaccagat atagcgtgtc
     5461 atactttgtt ttactcacgg atcaccattt taggtaaatt cctttagcaa ccgattcctg
     5521 tataacttca ttttgtgatt tattagtaga aatccaatag tttcccatat tactaagtgc
     5581 aaatttaaca cggggatatt ggcagacttc gagaacatct cgacaatcaa ctaaagcagt
     5641 attttcatca acatgcttat agcacttgaa gtttaaattt tttacatctg aggcgaatgc
     5701 tgcccaactc attggatcta gcgatgcttg taagggagga atcataaacg catctgagtt
     5761 atcaaaacgt tcaagctcaa ttttcgaatc tgaacgattt tgaatgagat aataggtttg
     5821 attacctgtt tcatttaaaa ttaaataatt ttcactataa ccaaaaccag tgacttcaca
     5881 gccacgggga aaaatcgaag cagcctggac tgcattggtc gcaaaagctg tgattaacag
     5941 cgttttagta aaataattaa ttaatgacat aatttcctca gaaaacaaaa ttaagatgct
     6001 attttaatca ttaaatagac aaagtctatc at
//
LOCUS       DBNCWK010000154         6027 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.154, whole genome shotgun sequence.
ACCESSION   DBNCWK010000154 DBNCWK010000000
VERSION     DBNCWK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.154"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            110..1354
                     /locus_tag="AC06IO_002574"
     CDS             110..1354
                     /locus_tag="AC06IO_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HIO9875742.1"
                     /translation="MSDLKNLILNSSILLDTSSTVQNDLNPEQLINVHEDSDGDSDLL
                     TPESFILLMAEMLSTNNTDTICEYEHQNSADNKVLQNLQFKEVEEDEQIQGLNQQKKS
                     LDNPLWHDLDLPVINEISGNQCEGQFESKLEKNVALTWINSENFQPPIIKPESQTDET
                     YKIWDSEITSIESGKLKPDFPKTDYDGNNINPYQKEAINQPGNDSSKNHQLIKGNDSF
                     AVLEDIKTNAEPGTEAIKSEVEATKVSTETPGSSFQMNQKAGLDIQPPVVSNSHIPKN
                     FSIPIHVNHSQWSEQLSEHIVWLGHQDVKSALIKIHPEELGPLEINVKVVKDNASVNI
                     STHSVYVKEIVDQAIPRLREMMAQQGINLSEVHIGTDTSSRQGSQKNHHAEIDLIQSA
                     EDKNKITSLTRHLPKGLVDYFA"
     gene            1391..2593
                     /locus_tag="AC06IO_002575"
     CDS             1391..2593
                     /locus_tag="AC06IO_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavA"
                     /protein_id="HIO9875743.1"
                     /translation="MSHNYFRTCLTTKDNKFFWQKPLASERFDKLIADRKFQKSIAHS
                     RKNQLMYSFIESKIASDYALLIDTKLREQLKQFTLDDFNDISGFERKEKSNSRQQSYF
                     KLRQELEFFLKNDIQQHKSRPDAQLNAFRRWINISDLLLRHHCYEGFLLVFVNLQLIA
                     DKQLIDGLPVSVRNNYNQLCQLSSPTGNHGALRHFMNAHQSEGDFTPLFFTYHAIGAL
                     DESLESLKDKEVLLKKQLKHLNKKLNHLRREVTPEVIDIIYDFLKNKQQIPKKMMERR
                     GHLIQLLEEVGCVGKQLKQIQINVQEQLEQRANLVGLIIKEQKTTPRTIPDYLEKTHN
                     IIQHRFNKQSIATVKLPNPLEPTTEKILSSSSLYKSKLLPNFWNRHGKSSSSYWEEVF
                     TPSYLNNR"
     gene            complement(2644..2961)
                     /locus_tag="AC06IO_002576"
     CDS             complement(2644..2961)
                     /locus_tag="AC06IO_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875744.1"
                     /translation="MKMVNAYVPRSFAIIKLTFLLFITISFSNVASASNTNTDQYLSG
                     NTQLAYFIGIHSYPGNYYWRGPVYYKNAYWTKWRYIGYGCQKSCLIDRWSGRVIRCAK
                     RCF"
     gene            3135..3986
                     /locus_tag="AC06IO_002577"
     CDS             3135..3986
                     /locus_tag="AC06IO_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9875745.1"
                     /translation="MAMNTKVILITGCSSGIGFDAVFALKKRGHRVIASCRKTEDVQK
                     LLNKGVEALLLDVSNPISIRSAFAQLLIMTSGHLDVLINNAGYGQVGALEDISRDILR
                     QQFETNVFGLQDLTNLAIPVMRGQGGGRIINISSILGVVSMPFRGAYNASKYALEGLS
                     DTLRLELSGSGIKVITIEPGPINSRFRDNAVDLSLQQIPMEKSYFKNQYRNMLSNFKQ
                     KKADSFFTRDTDAVIKKLVHAVESRKPKPKYPVTFPAYFLIGLKRFFTTRLLDRFILF
                     LSRKELG"
     gene            4528..5877
                     /gene="dsbD"
                     /locus_tag="AC06IO_002578"
     CDS             4528..5877
                     /gene="dsbD"
                     /locus_tag="AC06IO_002578"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIO9875746.1"
                     /translation="MRKLIDSFMILLLIFFTQAAWSYTPGFESSNPDTVMHFITGNSA
                     FVYLSAFFGLGILLAFTPCVLPMVPILSGIIVGQNSISTGKAFKLSMSYVTGMAITYA
                     GAGMLAGYMGSTIQTVMQRPVVITLFSVVFVAMALSMFGFFELKLPASLGNRLNKNNQ
                     LGKQRGYLSVGIMGILSTLIVSPCVTAPLIGVLSYIGQNGQVIMGGLILFVMALGMGV
                     PLLIVGAGYGTLLPKAGSWMLKIKQLFGFIMLGIAIWMLSRILNQTITHILWAGLLIV
                     ASISLGALKSQNNWRGYLAQGLGILALISGGIIFYNTAIPSVKIKQASVENNPFIKIN
                     TLNALQLQLEQAKKVRKAVFLDFSADWCSDCQEMDTKVFNQPEIQKAMSDWVSIKVDI
                     SDKNAEVDSIKKAFGIYGTPTMIFFNTSGEPITQMTSVGLISLSKMLQLLEQARHLS"
BASE COUNT         2004 a         1007 c         1134 g         1882 t
ORIGIN      
        1 ggttttacat gcgtgcaata ttttttaact atcccttaat tattttttca gtaagcttgc
       61 agatggtatg agttttgcta tatgttaatc ataacacagg gagtgtgata tgtctgatct
      121 gaaaaacctt atactcaatt cgtccatctt attggatacc tcttcaacag ttcaaaatga
      181 tctaaaccct gagcaattga ttaacgttca tgaagattct gatggcgaca gtgatttatt
      241 aactcctgaa tcttttatac tgttaatggc cgaaatgtta tcaacaaata atacagatac
      301 aatatgcgaa tatgagcatc aaaattcagc agataataaa gttttgcaaa atttgcaatt
      361 taaagaggtg gaagaggatg aacaaattca aggactcaat cagcaaaaaa aatcattaga
      421 taatccattg tggcatgacc ttgatttacc tgttattaat gaaatatcag gtaatcaatg
      481 tgaagggcag tttgaatcaa aactagaaaa aaacgtggct ttgacatgga ttaattctga
      541 aaacttccaa ccaccaataa ttaaacctga aagtcagaca gatgagactt ataaaatttg
      601 ggactcagaa ataacttcta ttgaatctgg caaactgaaa ccggattttc caaaaacaga
      661 ttacgatggg aataacatta acccttatca aaaagaggca atcaatcaac cggggaatga
      721 ttcatcgaaa aatcatcaat taattaaagg gaatgattct ttcgcggtat tagaagacat
      781 taaaacgaat gctgagccag gaactgaagc aataaaatct gaagtagagg caactaaagt
      841 gagcactgaa acacctggta gttcctttca aatgaaccaa aaagccggtt tagatattca
      901 acccccagtt gtttccaact ctcacatacc taaaaatttt tcaattccaa ttcatgtcaa
      961 tcattcacaa tggtcagagc aattatctga acatattgtt tggcttggtc atcaagatgt
     1021 aaaaagtgcc ttaattaaaa ttcatcctga agaattgggg cctttggaaa ttaatgtaaa
     1081 agttgtaaag gataatgcct ctgttaacat atctactcac agtgtttatg taaaagaaat
     1141 tgttgatcaa gcgattccca gattaagaga gatgatggct cagcaaggta taaacttatc
     1201 tgaagtgcat attggaactg atacaagctc ccgccaaggt tctcagaaaa accatcatgc
     1261 tgaaatagat cttatccaat cagcagaaga taaaaataaa atcacttcat taaccaggca
     1321 tctacccaaa ggactggtcg actactttgc ttagtttaac aaatcaattg ttggtaagga
     1381 gtttacaaca gtgtcccata attattttcg tacctgtctg acgacaaagg acaataagtt
     1441 tttttggcaa aaacctttgg catcagaacg gtttgacaag ctgattgctg atagaaaatt
     1501 tcaaaaatca atagctcaca gcaggaaaaa ccaattaatg tatagtttta tagagtctaa
     1561 aattgcctca gattacgcac tattgattga tactaaatta agagagcaat tgaaacagtt
     1621 tactctggat gattttaatg atatttcagg atttgaacga aaagaaaaat cgaattccag
     1681 gcaacaaagc tatttcaagt taaggcaaga gctggaattt tttcttaaaa atgacattca
     1741 acagcataaa tctcgccctg acgctcaatt aaatgcgttc agacgctgga taaatatatc
     1801 agatttactc ttaagacatc attgttatga aggctttctt ttggtatttg ttaatttgca
     1861 attaattgca gacaaacagt taatcgatgg cttacctgta agcgtaagaa ataattataa
     1921 tcaactctgt caattaagtt caccaacagg aaatcatggt gccttgcgcc attttatgaa
     1981 cgcacatcaa agtgaaggcg attttactcc tttatttttt acctatcatg ctattggagc
     2041 tttggatgaa tcgcttgaat ccttaaagga caaggaagtt cttttaaaaa aacaattaaa
     2101 acatctaaat aaaaagctta atcatttaag aagggaggtc actccagaag tgattgatat
     2161 catttatgac tttttgaaaa acaagcaaca aataccgaaa aaaatgatgg agcgtagagg
     2221 acatctgata cagctgttgg aagaggttgg ctgtgttgga aaacaattaa aacaaataca
     2281 aattaatgtg caagaacagc ttgagcaacg ggctaatttg gtaggattga taatcaaaga
     2341 gcaaaaaaca acaccaagaa cgataccaga ctatctcgaa aaaacccata atattatcca
     2401 gcacagattc aataagcaat ctatagccac agttaaacta cccaatcctc tagagccaac
     2461 aacagagaaa attctgagtt cctcaagttt gtacaaaagt aaattgctcc ccaatttttg
     2521 gaatagacat ggaaaatctt cctcaagtta ctgggaagag gtattcacgc caagttattt
     2581 gaacaataga taagagaatt agatgagctc tgcttcatac aagccctgtt tatatgaact
     2641 cggttaaaaa caacgtttgg cacagcgaat gactctacca ctccatcgat ctatcaggca
     2701 gcttttctgg caaccgtaac caatatatct ccattttgtc cagtaagcgt ttttataata
     2761 tacagggccg cgccagtagt agttaccagg gtagctatga atcccaatga agtaagcaag
     2821 ttgagtattt ccggaaagat attgatcagt attagtgtta ctggcacttg ctacattgct
     2881 aaatgatata gtaatgaaca atagaaaagt taatttaata atagcgaatg atctcgggac
     2941 ataagcgttt accattttca tgataaattc cttttatcgc aaatgacatt aaaattatag
     3001 ctcaataatg aatcaattgt tcctatatta ttactataaa cattttttta actaatattg
     3061 ctaccaaatg agttttatag tggtagtaat tggataaaga gcttagtacg atagttaaaa
     3121 attaggcata ggtcatggcg atgaatacaa aagttatttt gatcacagga tgctccagtg
     3181 ggataggttt tgatgctgtt tttgctttga aaaaaagagg acatagagtg attgcatcct
     3241 gcagaaaaac agaggatgtc caaaaattac taaataaagg agtcgaggct ctcttgctgg
     3301 atgtaagtaa ccctatttca atccgaagtg cattcgcgca gttgttaatt atgacatcgg
     3361 ggcacctgga cgtattgatt aataatgcag gatacggtca ggtaggtgct cttgaagata
     3421 tttctcggga tattcttcgc caacaatttg aaacgaatgt atttggtttg caagatttga
     3481 ccaatttggc tattccagtt atgaggggac aaggcggggg acgcataatc aatatcagct
     3541 ctatacttgg cgtagttagc atgccatttc gaggtgctta taatgcttcc aaatatgcat
     3601 tggagggctt aagtgacaca cttcggctgg aattgtctgg ttcaggtatt aaggtcataa
     3661 ccatagagcc tggtcctata aatagccgat ttcgtgataa tgcggtggac ttatctttac
     3721 aacaaatacc aatggaaaaa agttatttca agaatcaata ccgaaatatg ctgagcaatt
     3781 ttaaacagaa gaaggcagac tcatttttca cccgggatac tgatgcagtg ataaaaaaac
     3841 tggttcatgc cgtggagtca aggaaaccaa aacccaagta ccctgtaact ttcccagctt
     3901 attttttaat tggtttaaaa cgattcttta ctacccgttt attagatagg tttattttat
     3961 ttttatccag gaaggagcta ggttaaagca acttattggt aatattataa aatagtcata
     4021 agttggtgtt gatagttagc aactactgta cagacctttc ttaaaaagat actatagcta
     4081 ttatgaaact taaatacaga gtaattcatt acctgatact atgcagtatg gttttgttaa
     4141 tgataaaacc agcagtgttt cataacaata atcagcagac tcagagtaat acacaatata
     4201 tacattctat aacagaatgt cctcagagga cggcattgat gaattattgc tcgtccagtg
     4261 cctattacat tatttctgaa gaggaattat ctctcggtaa tgtcttatcc ttgctgacat
     4321 ttatccttta ttttttagtt attcaatcaa aatacaaatc gcctgtatcc ctaattttta
     4381 aaccaccgat tgcccactag aataaatttg tcaactttct cagtaggcct cttgccagcc
     4441 ctggctatgc aagaggtcca gtgtaggtca cttgtactct gatacaagta tctaccgttg
     4501 ttatttatta cttattggaa cattaaaatg agaaaattaa ttgattcgtt catgatttta
     4561 ttactgatat tttttactca ggcggcgtgg tcatatactc ctggctttga aagttctaac
     4621 ccggatactg tgatgcattt tattacaggg aatagtgctt ttgtttattt gagtgctttt
     4681 ttcggattgg gtattttgtt ggcttttacg ccctgtgttc taccgatggt tcctatttta
     4741 tcaggaatta ttgtaggaca aaattctatt tctacaggaa aagcctttaa attatcaatg
     4801 agttatgtga ctggaatggc tatcacttat gcaggagcag ggatgcttgc cgggtatatg
     4861 ggaagtacaa tccaaactgt aatgcaacgt ccggtagtaa tcactttatt cagtgttgtg
     4921 tttgtagcca tggctctttc aatgtttggc ttttttgaat tgaaactacc tgcaagcctt
     4981 gggaatcggt taaacaaaaa caatcaatta gggaagcaaa gaggttatct ttctgttggg
     5041 ataatgggaa ttttatccac tttaattgtt tctccttgtg ttactgctcc tttaattggt
     5101 gttttaagtt atattggtca aaatggccag gtcataatgg gcggattgat tttatttgtt
     5161 atggcattag ggatgggggt tcctctgctg attgttggtg cgggttatgg caccttactc
     5221 cccaaagcag gttcctggat gcttaaaata aaacaattat ttggttttat catgttggga
     5281 attgccatat ggatgttatc ccggattctg aatcagacca ttacgcacat tttgtgggca
     5341 ggattgctca ttgtcgccag catcagttta ggtgctttaa aatcacaaaa caattggcgt
     5401 ggttatttgg ctcagggatt gggtattctt gctttgattt caggaggtat tattttttac
     5461 aacaccgcaa taccttcagt aaaaataaaa caagcgtctg tagaaaataa cccatttatc
     5521 aagattaata ccttaaacgc tcttcaactg caattggagc aggctaaaaa agttagaaaa
     5581 gcagtatttc ttgatttctc agcggattgg tgcagtgact gtcaggaaat ggataccaaa
     5641 gtattcaatc aaccagaaat tcaaaaggcc atgtctgatt gggttagcat taaagtggat
     5701 atcagtgata agaatgcaga ggttgattct ataaaaaaag cttttggtat ttatggaacg
     5761 ccaacgatga tattttttaa tacatcggga gagcctatta ctcaaatgac ttcggttggt
     5821 ttgatttcac tatcaaaaat gcttcaattg cttgagcaag cgcgccattt gagttgaaat
     5881 aatagaaaat ttgctactga aatttgttct taataatttg ggtatggtta atatttgata
     5941 aataaatcaa attcaaactg tacccaaact attgaaaagt atgaatttga ttcttcgctc
     6001 ttagagttgc ttgtataagc aagaatt
//
LOCUS       DBNCWK010000155         5922 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.155, whole genome shotgun sequence.
ACCESSION   DBNCWK010000155 DBNCWK010000000
VERSION     DBNCWK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.155"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            53..559
                     /gene="cegC3"
                     /locus_tag="AC06IO_002579"
     CDS             53..559
                     /gene="cegC3"
                     /locus_tag="AC06IO_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC3"
                     /protein_id="HIO9875747.1"
                     /translation="MDLIFSLGVMIMPLTPPASPKTERRSEEDNQQTNQQGNEQGNQQ
                     PRSSILTLFGFTPNALVMPCPHGHGHFHIMDIQADDTLVFATMNGSPEIMSLLALQFL
                     MQNVNLNLDDMLAAETDAKEAEEKEEQELSSSESVNEEKEESEFSSSESENEEKEEEN
                     EESSRFTM"
     gene            906..1790
                     /locus_tag="AC06IO_002580"
     CDS             906..1790
                     /locus_tag="AC06IO_002580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIO9875748.1"
                     /translation="MTKTDKKIVVVTGCSKTSGVGYNLVCEMLKRGHQVIATVRNLET
                     SQINKANLPNSSNLDVKILDLCDENSIDRFIKEVLSQYQYIDALVNNAANVAIGPVES
                     ATTQDVLTTFQTKVFGPLSLIRGFIPVMRERKKGLLTTTSTIFTSVPVSIPGVAIYFS
                     ALAAFERLQESLAIELAPWNIKVINFQPGPIATELTRFEGSRANIVEKYYKNFTEQAY
                     QWFHQNTVFQNAAEVAKVFAEVIDMENPDLCYQSNLFGKEFVAKNKKDTTGREGMDKL
                     LEHFKNLPHDNPDDWKIQ"
     gene            2054..3148
                     /gene="potA"
                     /locus_tag="AC06IO_002581"
     CDS             2054..3148
                     /gene="potA"
                     /locus_tag="AC06IO_002581"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HIO9875749.1"
                     /translation="MTTPLIEIRQIYKSYGNTPILNNVSLNVNHGEFLTLLGPSGCGK
                     TTLLRLISGFEQPTQGEIFINGQCVNQLPPQKRDVHTVFQSYALFPHLSVFENVAFAL
                     RCKKTPNQEIRKRVFDALKLVQLESLAERNVKQLSGGQQQRVAIARAIINRPQVLLLD
                     EPLSSLDYRLRKAMQSELKQLQKTLNMTFIFVTHDQEEALSMSDRIVVFNHGHIEQIG
                     TPKAVYETPANLHVAMFIGEANIFNIQVHTVKDQNIITNIEGIQLSCKNTGNYQVNEW
                     LHLIVRPEDIRVWSLSEVEKTEGMLPGRIVDIIYKGSTVDLKVELSSGKIINASEFFD
                     EDDDKLEYTLHETVWVQWLPGWEVLLPYEG"
     gene            3138..3962
                     /locus_tag="AC06IO_002582"
     CDS             3138..3962
                     /locus_tag="AC06IO_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9875750.1"
                     /translation="MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPL
                     TLENYFDLFTPVFAKILLRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIP
                     FWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
                     LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYI
                     PNVLGGARSILLGNLIQNQFLVLSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR"
     gene            3959..4726
                     /locus_tag="AC06IO_002583"
     CDS             3959..4726
                     /locus_tag="AC06IO_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIO9875751.1"
                     /translation="MKTMTQRLFLSLVYAFLYIPIAILVLYSVNDAKFSLQWHGFSLR
                     WYKELFHDRGLWSAVFNSITLGLISSLIATTMGLLASINLFLFRTRHRQILYTLLLLL
                     IIIPDIVLGVALLIFFNITQVPLGFLSLLVAHITFSIPFVILTINSRINTLNPNIYFG
                     ALDLGASRYFALTRVLLPLLWPAVLSAFLLSFTLSFDDVIISYFVAGPDFTILPLTIY
                     SLVRAGVTPELNALCSITFGLSMILVIISHRLSGKLI"
     gene            4723..5745
                     /locus_tag="AC06IO_002584"
     CDS             4723..5745
                     /locus_tag="AC06IO_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIO9875752.1"
                     /translation="MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGI
                     KVNFSTYDSNETMYAKLRASQKTIYDVILPSAYFVERMQKQGMLQLLDHKLLPNLHNL
                     DEQFVKNDYDPGNRYSIPIIWGATGIFFNHNSVSNPPKKWQGLWDKKWRNQLMLLDDS
                     REIFAIALMSLGFDPNDSNPNHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGS
                     AWNGDAFKAHTENKAIEFIYPQDGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSA
                     QIALKEGHAITNAAGRALLPLPIKNNPVVYPPDEILKKGYFQRDIGEETLALYNQYWE
                     QLKLAF"
BASE COUNT         1814 a         1133 c         1078 g         1897 t
ORIGIN      
        1 gacgccgaaa agatagattt ttaattagat tatctttttt gggcagtatt ttttggattt
       61 aatttttagt ttaggagtaa tgattatgcc tttaaccccc cccgcttcac caaaaactga
      121 aagacgttct gaagaagata atcaacaaac taatcaacaa ggaaacgagc aaggaaatca
      181 acaaccaaga agcagtatat tgactctttt tggttttact cctaatgctc tagtcatgcc
      241 atgccctcat ggccacggtc acttccatat tatggatatt caggcagatg atacattagt
      301 atttgctact atgaatggca gtccggaaat aatgagtcta ttggcgcttc aattcctgat
      361 gcaaaatgtc aacttaaatc ttgatgatat gctggcagca gaaacagatg cgaaagaagc
      421 agaagaaaaa gaagaacagg aactgtcatc gtctgaatca gtgaatgaag aaaaagaaga
      481 atcagaattt tcatcttctg aatcagaaaa tgaagaaaaa gaagaagaaa acgaagaatc
      541 aagccgtttt acaatgtaat atgcctgccc tcttttgaag ggacaccccg tatttacctg
      601 aggtaaatac gggatttttt taattcctct gtcttcaata ttgtcaatct ttatcagaat
      661 aaacgtccca ataatcaaac aagagcaatt aatctcggta ataaactata tatcactgaa
      721 tatcctatat tttctcaacc aatatttttc tgctttcaaa aaatcagtta agagtttgga
      781 aaatcaataa taaatgaccg aattaaaaac ctttttaaca aagagattgg ggtttttcgt
      841 aagttctgaa taaataaaag ggctatactc agtttattac tctaaaagat cattaggata
      901 gggacatgac gaagacggat aaaaaaatag tggtggtcac aggttgttca aaaacatccg
      961 gcgtaggata taatcttgta tgtgaaatgc taaagcgcgg gcatcaagtc attgcaacag
     1021 tgagaaatct tgaaacaagc caaattaaca aagcaaacct cccaaactcc agcaatttgg
     1081 atgtaaaaat attggatctt tgcgatgaaa attcaattga ccgctttatt aaagaagtct
     1141 taagtcaata tcaatacatt gacgccttgg ttaacaatgc tgctaatgtg gctattggcc
     1201 ctgttgaaag cgcaaccact caagatgtat taacaacttt tcaaaccaaa gtatttggcc
     1261 cattgtcttt aatcagggga ttcatccctg taatgagaga aaggaaaaaa ggtttattaa
     1321 caacaacatc cacgattttt acctctgttc ccgttagtat tccaggtgtc gcaatctatt
     1381 tttcagcact tgcagcattt gaacgattac aagaatcctt ggcgattgag ttagccccgt
     1441 ggaatatcaa agtaattaat ttccaacctg gaccaattgc gacagaattg acccgatttg
     1501 aaggctctcg cgctaatatt gttgagaaat actataagaa ctttactgaa caagcttatc
     1561 aatggttcca tcagaatact gtttttcaaa atgcagcgga agtagccaag gtatttgctg
     1621 aagttattga tatggagaat cctgatttat gctatcaaag caatcttttt ggaaaagaat
     1681 ttgttgcaaa aaataaaaaa gatacaacag ggcgtgaggg aatggacaaa cttctggagc
     1741 atttcaaaaa tttacctcat gacaatcctg atgattggaa aattcaataa atgatttact
     1801 ggataaagtc atactatccg ccgcgaaatc tctgtgcata agcaaaaaat cctcagtggc
     1861 ttgacctgag cattggatta tgttgataga tggttgcccg cttatgcggg catgactgat
     1921 ttttttgctc atgtacaaaa cacaaaaata gacaatagtt gactaatttt ttaatattgg
     1981 gaacaaagtt tgtgattgac tggacaagat aagtcacagc acacataatt gcagttttta
     2041 tttaataaag cagatgacaa caccgctcat tgaaatccgt cagatttata aatcatacgg
     2101 caatacacca attttgaata atgtttcgct aaacgtcaat cacggtgaat ttttaacctt
     2161 actgggccct tcaggttgtg gaaaaacaac ccttcttcgg cttatatctg gttttgaaca
     2221 accaacgcaa ggtgagattt tcattaatgg tcaatgtgtt aatcaattac cacctcaaaa
     2281 acgggatgtg catactgttt ttcaaagtta tgcccttttc cctcatttat ccgtttttga
     2341 aaatgtcgca tttgccttgc gctgcaaaaa aacgcccaac caggaaatca ggaagcgagt
     2401 ctttgatgcg ttaaaacttg tacaacttga atcgttagct gaaagaaatg tgaagcagtt
     2461 aagtggaggc caacaacaac gtgttgcgat tgcacgagca atcatcaaca gacctcaagt
     2521 actgttgtta gatgagccat tgagttccct tgactatagg cttaggaagg caatgcaatc
     2581 cgaattgaaa caattacaaa aaaccttgaa tatgacgttt atttttgtaa cgcatgatca
     2641 ggaagaagcc ttatcaatgt ctgaccgtat tgtcgtcttt aatcatggcc acattgaaca
     2701 aattggtact cccaaggctg tctatgaaac ccctgccaac ctgcatgttg ccatgtttat
     2761 tggcgaagcg aatatattta atattcaggt tcatacagta aaggatcaaa acatcataac
     2821 aaacattgaa ggaatccaac tatcctgtaa aaacactggc aattatcaag tcaacgagtg
     2881 gcttcatttg atcgttcgtc cagaggatat tcgagtttgg agtttatccg aagtagaaaa
     2941 aacagaagga atgttgcctg gtcgtattgt tgatatcatt tataaaggct ctacggttga
     3001 tctcaaagtg gaattgtcat caggcaaaat catcaatgct tcggagtttt ttgatgaaga
     3061 cgatgataag ctggaataca ctctccatga aaccgtttgg gtacaatggt tgcctggctg
     3121 ggaggtttta ttaccttatg aaggctaaat catctgcact gtatttattg tatttttggc
     3181 taattatttt tggcttcatt cctttgttga tggtgctggt tgccagcttt ttatcccaag
     3241 acagtattca tcttgttacc ttgccattga cattggaaaa ttattttgac ttgtttactc
     3301 ctgtttttgc aaaaatcctt ctgcgatcct tgctaatagc ttgtgtcacc accctccttt
     3361 gcttgctcat cgcttatccc tttagttatc ttatgattaa atcaaaacac caatctattt
     3421 tattgctgtt gattatcata cctttctgga caagctcatt gataagaact tatgctttga
     3481 ttgctatcct taaatacaaa ggcgtgataa atgccctatt gctgaaatta cacttgattg
     3541 atgttccctt gtcactactc tataccaatt ttgcggtcgt tattggcttg gtttacaatc
     3601 ttttcccttt catggtccta cccattttta ctaatatgga acggttcgat ttcaggttaa
     3661 ttgaggcagc taaagatcta ggggcaaaca agtggtctat atttacacga gtatttttgc
     3721 ctaacacagc ccctggagtc ttatcaggtt gtttgctggt cttgttacca gcaatgactt
     3781 tattttacat tcctaatgta ttaggtggcg ctcgttccat tttattggga aatttaatcc
     3841 aaaatcaatt ccttgtcctg agcgattggc ctcagggagc cgctaccagt gtgctattaa
     3901 cttccgttct gcttatttta atgttcattt ttcggcgaca gaacaatgag gtcattcgat
     3961 gaaaacaatg acacagcgcc tgtttctttc tttagtttat gcttttcttt atatccccat
     4021 tgcaatactg gtgctctatt cagtaaatga tgccaaattt tcattacaat ggcatgggtt
     4081 ttctttgaga tggtacaaag aactatttca tgatcggggc ttgtggtctg ctgttttcaa
     4141 ttctatcacc ctggggctaa tatcctctct tatcgctaca acaatggggc tattggcctc
     4201 aatcaactta tttttatttc gtacaagaca ccgccagatc ctgtatacct tattactatt
     4261 acttattatt attcctgaca tagtcctggg tgttgcctta ttgattttct ttaatattac
     4321 ccaagtaccg cttggcttct tgagcctttt agttgcccat attacattct ccattccttt
     4381 tgttatcttg accattaatt cacgaataaa cactttaaat cccaacattt attttggcgc
     4441 cctcgatctt ggcgccagtc gttattttgc tttaacgaga gtcttgcttc ctttattgtg
     4501 gcctgcggtt ttaagcgctt ttttactaag tttcactttg tcatttgatg acgtcattat
     4561 cagctatttt gtggcaggcc ctgattttac tatcctgcct ttgaccatct actctttagt
     4621 ccgtgctggc gtcacgcctg aactcaatgc attgtgttca attacgttcg gtttatcgat
     4681 gattctcgtt attatctctc accgcttgtc aggtaaattg atatgaaaat tttttcattg
     4741 ctgctcgctc ttcttacatt taaggcgttc tccaatccag tagtcaatgt ctatgcctgg
     4801 ggaggtgaaa tccctaaaaa aataattcat caattcgaac aagagtcagg aattaaagtc
     4861 aatttttcga cttatgatag caatgaaacc atgtatgcca agctaagagc cagtcaaaag
     4921 accatttacg atgtcatcct cccttccgct tattttgtag agcgcatgca aaaacaaggc
     4981 atgttgcaat tactcgatca taaactgctt ccgaatcttc ataatctgga cgaacaattt
     5041 gttaaaaatg attatgatcc gggtaaccgt tatagtatac ccatcatctg gggagcaacc
     5101 gggattttct ttaatcataa ttccgtatcg aacccgccaa aaaaatggca aggcttatgg
     5161 gataaaaagt ggcgtaatca gctaatgtta cttgatgatt cacgcgaaat atttgccata
     5221 gccctgatga gtttagggtt tgatcccaat gatagcaatc caaaccacat aaaagcagcc
     5281 tatgaacatt tattgcaatt ggtacctaat attaagttat ttgctagtga tagtattcaa
     5341 gccatcatga tagatgagga tgcttttatt ggatcagcat ggaatggcga tgccttcaaa
     5401 gcacatacgg aaaacaaagc cattgagttt atttatcctc aagatggttt tgtgatttgg
     5461 gtagattgtt tggctatccc taaaaacccc ccacacctga aagaggcgta tcaattcatt
     5521 aattttattc tgaaaccgga aacaagcgct caaattgcac taaaggaagg acatgcgatt
     5581 accaatgccg caggaagagc attattgcca ttacctataa aaaataaccc tgtggtttat
     5641 ccgccagacg aaatactaaa gaagggttat ttccaacgcg acatcggcga agaaacacta
     5701 gcgctttata atcaatattg ggaacagtta aaacttgctt tttagttttc tgttgcaaag
     5761 tagcgctaaa tatccagatg aagtacttca ttaactcaac gaattaaaat tcagaaagca
     5821 agattgaatg cttactttat attcaaaaag aaaataatta tgcttaattt gtataaattt
     5881 aatctatttt gtggtcttat ttgtctcact ttttacatat ta
//
LOCUS       DBNCWK010000156         5918 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.156, whole genome shotgun sequence.
ACCESSION   DBNCWK010000156 DBNCWK010000000
VERSION     DBNCWK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.156"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            32..2380
                     /locus_tag="AC06IO_002585"
     CDS             32..2380
                     /locus_tag="AC06IO_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIO9875753.1"
                     /translation="MRYLLLLPGVLIAIVTSVNANTSLKPSIAQPTACVNSLFTATGN
                     QHWKSIVLKLTNNCNQAVDFQNSTVSFQTTAALNTSFWGDFSPLSYPDNALNISSQPQ
                     SGGNYLATLNLHFPSYPGANSKLPVGSSISIKYGAVTDSHIEGTANVYLSTTVESGSI
                     QLINAAAKPTNVSQGYALVHVTMNGQSVSDVQLPWETSKTLSGFAAGNYAISAETVTD
                     SNGNLYLGQANPSMVNVIANQTTSSTINYARVQETGKIKIHVQNLPGELSNYNENPTV
                     LITQSQSGNSRSQHAVWGNTTTVAELKEGSSYQFSTPAIQYNDYNCWPTFTPSSLVAS
                     AANVPTTNLTYQCAQIVKDNVTINVSGAPSSLASLKIILTPNDGSQTVEQTIDLANGV
                     GSSAISLTDGVIYTLSSEGVPGYTVQFSPQPLTATENATVNITLSPVTAAKGRIIGYV
                     PGWKTPPAAQELASAGYTHVMIAFGVFSTNTPGVIVPAFETITKEYIQSLHQAGIKVI
                     LSLGGALTSIPNTTVDFHQVLVASSSPEAFKQTFINSLKELISQYGFDGFDTDIEHGI
                     NASGSFSQPQGDIAVLASIINTMYSQNSSLLITLTPQVANIAATSGFDQTWGNYASLI
                     MQTHQSLAWVGIQLYNTGCAFGIDQVCYGPTPTDTPDFSVAMATDLLENWPATVNGRP
                     TGFQPYISYLRPSQIVIGYPSPNASGGSDGSPVTPTTTIKRAIQCLKTAIAGNTSCGV
                     YVPPRAYGNIGGVFNWEVTYDKNNQFKFAKELKNCAINGVCE"
     gene            complement(2490..2963)
                     /locus_tag="AC06IO_002586"
     CDS             complement(2490..2963)
                     /locus_tag="AC06IO_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIO9875754.1"
                     /translation="MAIYKFEISTMQNGNIQISAKHKSTGEIHGYVQIASSARLESRN
                     LLEIDQLYVDNNARGHGLGSELASRAIKYALKMDKGIHMMIQPGSDVFWKKYFNSRFD
                     GKRITLFGGETLNTNLADGKVFEISKESLVANYKFPQDRHLRDRDMEKICAKLSH"
     gene            complement(3055..4170)
                     /locus_tag="AC06IO_002587"
     CDS             complement(3055..4170)
                     /locus_tag="AC06IO_002587"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIO9875755.1"
                     /translation="MSRYVCKWKLIYTFFIFIVFNNCLLAFASPSLTKQLHTILKDNL
                     SDSLTPGAVLLISSPDLGTISVAAGLADKENDISMNITDSFRLASMSKTFLAVTVLKL
                     VADKQLNLDDHIADLLPDSIDIERIPNGDEVTVQQLLQMRSGIPNYTDYDSYSDLIDT
                     MADKEWTPEECIKLVYDQKPDFKPGTAYEYSNTNYLLLQLIIENLTGESYAESIKKYI
                     LKPIRLKNTYIEKQEFDDDHHYLSTHGYTLEDGKVVDVTDYDDGFGLADGGIISTAED
                     INIFVQALLKDKILLSPNYLTMMLTFPDDYGFGIGREEINGEIAWSHNGASSGYQGQY
                     YYFPDRQLTVVILTNYFDSDIIEDIASQTLSTIDNSE"
     gene            4438..5496
                     /locus_tag="AC06IO_002588"
     CDS             4438..5496
                     /locus_tag="AC06IO_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIO9875756.1"
                     /translation="MVNKICLSFALLISAPSILLAQDKLIFAVDIIRHGDRTPIVALP
                     TVNYQWQEGLGQLTAEGMQQEYKMGVAFRKKYIEELHLLPEHYEYGTIYVRSTDYART
                     LMSAQSLLMGLYPPGTGPSIPAGTSALPHAFQPIPVFSAPSKYDEVIIQQVDRKERKK
                     LMEQYVFSTREWQQKNNELKDKYPLWSRLTGINIDTLEDLETVGHTLYVHQIHNAPMP
                     EGLASNDIETIINSAEWAFMAQEKPQQIANVYSSKLMTNIADYLNSGSMKKSKLKYVL
                     LSAHDTTIASVLSFLGAPLEKSPPYASNVNFSLYDNGANYYTVKITYNGNPVLIPACG
                     GSVCELQQLVNLVHDSKN"
BASE COUNT         1784 a         1166 c         1099 g         1869 t
ORIGIN      
        1 tttttttcca atcacaaatg aggagggatt gatgcgatat ttattattac tgccaggtgt
       61 tttaattgca attgtgacaa gtgtcaatgc caatacctct ttaaaaccct caattgccca
      121 gcctacagcc tgtgtaaact cactttttac agcaacggga aatcaacact ggaaatcaat
      181 cgtattaaag ttaaccaata attgcaatca ggcagtcgat tttcaaaatt caactgtctc
      241 ctttcaaaca acagcagctt taaatacctc tttttggggg gatttttctc ctttatctta
      301 cccggataat gctttaaata tttcttctca acctcaatct ggcggaaact atctagccac
      361 tttaaacctg cattttccca gctatccggg tgctaatagc aagttacctg tcggaagttc
      421 tatttcaatt aaatatgggg cagtcactga tagtcatatc gagggaacgg ctaatgttta
      481 tttaagcaca acggttgaat caggaagcat ccaattaatc aatgctgctg caaaaccaac
      541 aaatgtgtca caaggatatg cattagttca tgtcacaatg aatggtcaat cagtcagtga
      601 tgtgcaatta ccatgggaaa cttcaaagac tctatctggg tttgctgcag gtaattatgc
      661 tatttctgct gaaacggtta ctgacagtaa tggcaatctt tatctgggac aggccaatcc
      721 cagcatggtg aatgtgattg caaaccaaac aacgagctcg actattaatt atgctcgggt
      781 acaagagaca ggcaagatca agatccatgt gcagaacctc cctggcgaat taagcaacta
      841 caatgaaaat ccaactgttc taattaccca gagtcaatca ggaaattcac gctcacagca
      901 tgccgtttgg gggaacacaa cgactgttgc tgagttgaaa gaggggagta gttaccaatt
      961 ctcaactcct gcaattcaat ataacgatta caattgctgg cccactttca caccatcttc
     1021 cttagtggca agtgctgcaa atgttccaac taccaattta acctatcagt gtgcccagat
     1081 tgtgaaagat aatgtgacaa ttaatgtcag tggcgctcca tcctccctgg cttcattgaa
     1141 aattatttta actcccaacg atggttcaca aacagtagaa cagacaatag atttggccaa
     1201 tggtgttggt tcatctgcca tttctttaac tgacggggtt atttatacac tttcaagtga
     1261 aggtgttccc ggctatacag tacaattttc gccccaacct ttaacagcaa cagaaaatgc
     1321 aacagtaaac atcactttat ctccggtaac cgctgcaaaa gggcgtatta ttggttacgt
     1381 accgggatgg aaaactccac ctgccgctca agagttggct agcgcgggtt atactcatgt
     1441 catgattgct ttcggcgtat ttagtaccaa tactccgggt gttattgttc ctgcttttga
     1501 aacaataacc aaagaatata ttcagtctct tcatcaagcc gggattaaag ttattctttc
     1561 tttaggtggt gcgttaacta gtattcccaa tacaacagta gattttcacc aggttttagt
     1621 agcttcttct tcaccagagg catttaaaca aacatttatc aactctttaa aggagttaat
     1681 ttctcaatat ggttttgatg ggtttgatac agatattgag catggtatta acgctagcgg
     1741 ttccttttct caaccacagg gtgacattgc tgtcttagca agcattatca atacgatgta
     1801 cagccaaaat tcttctctgc taattactct gacacctcaa gtggctaata ttgctgcaac
     1861 aagcggtttc gaccaaacct gggggaatta tgcctcttta attatgcaaa cccatcagtc
     1921 tttagcgtgg gtaggtatcc agctttacaa tacaggatgt gctttcggaa ttgatcaagt
     1981 atgctatggt cctacaccaa ctgatacccc tgatttttca gtagctatgg ctaccgattt
     2041 attggagaat tggccagcaa cggtcaatgg acgtcctaca ggatttcaac cttatattag
     2101 ctatttaaga ccttcccaaa ttgtcattgg ttatccatct ccaaatgcta gtggtggcag
     2161 tgacggttca ccggttactc cgacaaccac aatcaagcgg gctattcagt gccttaagac
     2221 agcaattgcc ggtaatacca gttgtggtgt ttatgttccg ccaagagctt atgggaatat
     2281 cggtggtgta tttaactggg aagtaactta tgataagaac aatcaattca aatttgcaaa
     2341 agaattgaaa aattgtgcta ttaatggtgt ttgtgagtaa tcaatttgac gtgtcaggct
     2401 ctttgagcct gacacttaaa tcttgaaata ttgtttatca tgtattatct gtttgatcca
     2461 atatatttta attttgtata atcatccgct caatgagaga gcttagcgca gattttttcc
     2521 atatcgcgat cacgcaggtg tctatcttga ggaaatttgt aattggcaac caaactttct
     2581 ttagaaattt caaatacttt tccatcagct aaattagtat ttaaagtttc tccaccaaac
     2641 agggtaatgc gttttccatc aaaacgagaa ttgaaatatt ttttccaaaa cacatccgag
     2701 cctggttgaa tcatcatatg aatgccctta tccattttaa gagcatattt aatggctctt
     2761 gaggcgagtt cactccccaa tccatgtcct ctggcattat tgtcaacata aagctggtct
     2821 atttctagca aatttctgct ttctaacctg gctgatgagg caatttgaac atagccgtgt
     2881 atctctcctg ttgacttgtg ttttgcggat atctgtatat tgccgttttg catagttgag
     2941 atttcaaatt tgtaaatagc catattttgc ccccataaaa gtccagataa ttttcactta
     3001 gtataacata taatctattt tgcggacttc cattagatga gtgcagttaa gcagttattc
     3061 agaattgtca atcgtactga gagtttgaga tgctatgtct tcgatgatat cactgtcaaa
     3121 atagttggtt aaaataacaa cagtcaattg cctgtctgga aaatagtagt actggccttg
     3181 gtatcctgag cttgccccat tatgggacca ggctatctct ccattaattt cttcacgtcc
     3241 tattccaaaa ccgtaatcat ctggaaaggt aagcatcatt gttaaatagt ttggagataa
     3301 aagtatttta tctttcaaca acgcctggac aaagatatta atatcttcag cagtactaat
     3361 tattccacca tcagccaagc cgaaaccatc atcatagtct gttacgtcaa ccactttgcc
     3421 atcttccaag gtatagccat gtgttgataa ataatggtgg tcgtcatcaa attcttgctt
     3481 ttctatatat gtatttttta aacgaatagg tttcaggatg tatttcttaa ttgattcagc
     3541 ataggattct ccggttaagt tttcgataat taattgcagc aacaaataat tagtattgct
     3601 gtactcataa gctgttcctg gcttaaagtc aggtttttga tcgtagacta gtttaatgca
     3661 ttcttctggt gtccattcct tgtctgccat agtatcaatg agatcactgt aagaatcata
     3721 atccgtataa ttaggaatgc cgctacgcat ttgcagtaat tgttgcactg ttacctcatc
     3781 tccattcggt atacgttcaa tatctatact atctggcaat aagtctgcaa tatgatcatc
     3841 aagatttaac tgtttatctg ctaccagttt taagacagtt acagccaaaa atgttttact
     3901 catgcttgca agacgaaaac tgtccgtaat attcattgaa atatcatttt ccttatctgc
     3961 caaccctgcc gcgacggaaa ttgtgcccaa atcaggactt gatatcagta atactgctcc
     4021 aggggttaat gagtctgaaa ggttgtcttt taagatggta tgtaattgct tggttaagga
     4081 agggctcgca aaggcaagta gacagttatt aaagacaata aaaatgaaaa acgtataaat
     4141 caatttccac ttgcatacat aacggctcat tgcaactcca atgtggcaag ggttttctca
     4201 cccaccaaat gagctatatc ttaattctag tcgatttcag gataatttct ttacttcctg
     4261 tataaaatag atttctcctc caggtttcaa atgaatcagg gaatacaaaa gccgatgtct
     4321 tgatttttat ttgtgtcttc gtcatagtgc gaaaccgaga ttaaccaacg gtggcaagat
     4381 acaacaagaa ttttttacga agcctcatgt ttactttaac tcaaggagta tgaatacatg
     4441 gtcaacaaga tttgcctgag tttcgcacta ttaattagcg caccctccat tttattagca
     4501 caagacaaac ttatttttgc tgtggacatt attcgtcatg gcgacagaac gccgattgtt
     4561 gctttgccta ctgtaaatta ccaatggcaa gaggggcttg gccagcttac ggcagaaggc
     4621 atgcaacaag aatacaaaat gggtgtggca tttcgcaaaa aatatattga agagttgcat
     4681 ttattacctg aacactatga atacggaacc atctatgtcc gatctacgga ttatgcacgt
     4741 actctgatga gcgcccagtc attgttaatg gggctatatc cacctggaac tggaccatca
     4801 attcctgcag ggacctccgc tttacctcat gcttttcaac ctattcccgt atttagcgca
     4861 ccgtctaagt acgacgaggt tatcattcag caggtagatc gtaaggaacg gaaaaagcta
     4921 atggagcaat atgttttttc taccagggag tggcaacaaa aaaataatga attgaaagac
     4981 aaatacccat tatggagccg tctgactggc attaatattg atactctgga ggatctggaa
     5041 acagttggtc ataccttata tgttcatcaa atccataatg ctcctatgcc ggaaggatta
     5101 gcttctaacg atattgaaac aattatcaat tctgctgagt gggcttttat ggcacaagaa
     5161 aaacctcaac aaatagcgaa tgtttatagt tcaaagctga tgactaacat agctgattat
     5221 ctgaattccg gaagtatgaa aaaatctaaa ctgaaatatg ttttgctatc tgctcatgat
     5281 actacaatag ccagtgtatt aagtttttta ggtgctcctt tggaaaaatc cccaccttat
     5341 gcctcaaatg ttaatttttc tctctatgat aatggagcaa attattacac agtaaaaatt
     5401 acctataatg gtaatccggt acttattcct gcttgcggtg gctcagtatg tgagttacaa
     5461 cagcttgtaa atttggtaca tgattctaaa aattgagtgt aatcaggaga tatttggctt
     5521 gggtgagtaa tcctaaccat ctcaaactat ctgttgttgc aaaacagcaa tagcagatgg
     5581 tttgaagtaa ttcaaataat cgatcaattc caaaagatct gggcacatga atgggatact
     5641 ctaataatca aaactctgtt caagataaga tttgttgcgg tcgtgctggg aatgcaaccc
     5701 ttgatagagt acacacaaat ccggatggaa aaatttataa attaaatatg acctgacaga
     5761 tacctttgaa tagctggtaa ctgtcagata aaactagaat aactaagtct tatatttgct
     5821 aagattagtt tttagccttg gtttcaagat tcaacttttg atttaaatca atatctaatt
     5881 cttgatttgg attaatattc gttggtttag aaaataat
//
LOCUS       DBNCWK010000157         5889 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.157, whole genome shotgun sequence.
ACCESSION   DBNCWK010000157 DBNCWK010000000
VERSION     DBNCWK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.157"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..148
                     /locus_tag="AC06IO_002589"
     CDS             <1..148
                     /locus_tag="AC06IO_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HIO9875757.1"
                     /translation="TGKTLPVTLISQLSQKRIGIECMSIGAACRTYNVLLSEHRAVVA
                     GFIF"
     gene            complement(189..2393)
                     /gene="relA"
                     /locus_tag="AC06IO_002590"
     CDS             complement(189..2393)
                     /gene="relA"
                     /locus_tag="AC06IO_002590"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HIO9875758.1"
                     /translation="MVRVKDTTPLTPDGSIDVEMWLHHLGSKGYLDNLELVRAACTLS
                     QLAGQDHATETGQTCLQQGLSMADLLADLEVDQETLAAAIIFENVHYADLSIDDVEEQ
                     LGHNIAKLVKGIEKMSAMNNFQALNKYPQNKNQIDNIRKMLLAMVDDVRVVLIKLAEC
                     LCILRTAGHLSETVRKQLATEAMEIYAPLANRLGIGAIKWEMEDLAFRHLHPEEYKAI
                     AKGLKAKRLERDSFVNRIVEQLEHQIRATGARHFAVYGRSKHIHSIYKKMKRKNVSLD
                     EIYDATAVRILLDTEAQCYEVLGMVHTLWKQIPAEFDDYIINPKSNGYQSLHTAVEGP
                     EGRVFEVQIRTFHMHDLAEMGVAAHWKYKEGAVSQKESHERKIEWLRDVLAWHQEMAA
                     NKGVAENVTTEFLEDRVYVFTPDGDVLDLPHGVTPLDFAYHVHSNLGHRCRGAKVNGH
                     IVPLTYQLKTGDRVEVLTGKEIKPSRDWINPHLNYLKTARAKAKVLHWFKMQDYEDNV
                     RDGRELLEKELKSLGIKSDKLNEVITALNYKKADDLYASLGRGDIKIGQIVNRLAPPD
                     ISEQNLQKFVRQQQKPEVTGSDLRIEGVGNLLTFMARCCQPVPGDEIVGYITIGRGVS
                     VHRKDCPNIIHASEKQKRRFIQVNWGTATRENYVVDVLIKAFDRSELLKDVTSLLSNE
                     KAHVYALQTHSNKHENMAYITLTVEIDGLNSLSRLLTKLEQIPNVLEARRQL"
     gene            complement(2399..3733)
                     /gene="rlmD"
                     /locus_tag="AC06IO_002591"
     CDS             complement(2399..3733)
                     /gene="rlmD"
                     /locus_tag="AC06IO_002591"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIO9875759.1"
                     /translation="MRKVKPKLNLTSQTARIVNLSHDGRGIARINGKATFIQGALPGE
                     VVEFQYTRVKKDFDEGKLLSIVEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQS
                     HLLDLLSRYGHTAPQTVLSPLTSHPWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
                     TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQ
                     DELKIRKFAQQFQYKIFLQPGGLDSVFCFYPSDAHAYLNYELPDYQITFQFHPNDFTQ
                     VNAELNRKMVKQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCSRVIGVEGNRNMVER
                     AYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDAIDP
                     ERIVYVSCNPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG"
     gene            3854..5110
                     /locus_tag="AC06IO_002592"
     CDS             3854..5110
                     /locus_tag="AC06IO_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HIO9875760.1"
                     /translation="MLKNITRALSLSLLISHNTCANEASSIDTVSQPTTVQFLEKFGA
                     GFANLEHKNLGDRVQLYLPGHNSKTGIVKLRLPNGSKLSYGEIVMYGGDMFGNPAKPI
                     SNCSAKNRLACFQAQFDALGTLGNKQDKRCSNPLNQITHLDRFFTNLAEEIEKSKQNG
                     TNDWDYYNQHDSAISKELNKLTCGGSIISGFIPFGNYIKLAEVNYDHFVPDSLVAYKT
                     GHRFALETALKAYEKKQQGLIDEAHKLLELAYAQNAFANHYLTDSFSAGHMRTPRRAI
                     GHDILLPSVLNLLIANLMHNEDNQHGLNVVNHEGISWLAYGDGYLYKPEAELQRVIIL
                     DAMQRSADSVHSTFMNGVIPEHYPEMDVFPDYSKIEQINDTAPLFKVENGVLLKRVNN
                     KDHYDHHWTKYWSGLVTLIEFQVNKT"
     gene            5139..>5889
                     /locus_tag="AC06IO_002593"
     CDS             5139..>5889
                     /locus_tag="AC06IO_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875761.1"
                     /translation="MKKNIIMFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKL
                     ALISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARII
                     QGLGGSLTVPVGRLIILRTCKRHELITKMSIVVMVASLGMLLGPLLGGIITDYFSWRW
                     IFWVNIPVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKT
                     DISYSVSIILLSALLLIGYTRHSRNKSNPIIK"
BASE COUNT         1752 a         1244 c         1044 g         1849 t
ORIGIN      
        1 aacaggcaaa acgctgcctg tcactctaat cagtcaatta tcgcaaaaaa gaattggtat
       61 cgaatgcatg tcgataggcg ccgcttgtag aacttataac gtcttattga gcgaacacag
      121 agcagttgtt gcagggttta tcttctaata tcaatttttt gagaaggctc tggctaaata
      181 taccaaagct ataattgcct tcttgcttcc agcacgttag gaatttgctc caatttagtt
      241 aataagcgcg acaaactgtt taaaccatcg atctcaacag ttaaagtgat ataagccata
      301 ttttcatgtt tattgctatg ggtctgtaaa gcataaacat gggctttttc atttgaaagc
      361 agcgaagtaa catccttcaa taactcggaa cgatcaaagg ccttaatcag cacatcaaca
      421 acataatttt cccgtgttgc tgtaccccaa tttacctgaa taaaccgtcg cttttgcttt
      481 tcactggcat gtataatatt aggacaatct tttcgatgaa cggaaacccc ccttccaatc
      541 gtgatgtatc ctactatttc atcaccagga acaggttggc agcatctcgc cataaatgtc
      601 aataagttcc caactccctc aatacgaaga tcactccctg tgacctctgg tttttgttgt
      661 tgtcttacaa acttctgtaa attttgttct gaaatatctg ggggtgccaa tcgattaaca
      721 atctgaccta ttttaatatc acctcgcccc aaactggcat aaagatcatc cgccttttta
      781 taattcaatg ccgtaatcac ttcattgagt ttatccgatt taatacctaa agactttaat
      841 tccttctcca ataactcacg accatctctc acattatctt cataatcttg cattttaaac
      901 caatgcaata ctttagcctt agctcttgca gtctttaaat aattcagatg aggattaatc
      961 caatcccttg atggcttgat ttcttttcca gtcagtactt caaccctgtc tcctgttttc
     1021 aattgataag tcaatgggac aatatgacca ttaaccttgg cacctcggca acgatgtccc
     1081 aaattactat gcacatgata agcaaaatcc aaaggagtca ccccatgggg taaatccaac
     1141 acatcgccat cgggagtaaa tacatacact ctgtcttcaa gaaactcggt cgtaacgttt
     1201 tctgcgacac ctttattagc tgccatttcc tggtgccacg ccaaaacgtc tcttaaccat
     1261 tctattttgc gctcatggct ctctttttgt gataccgcgc cctctttata tttccaatgg
     1321 gctgccacac ccatttctgc taaatcatgc atatgaaaag ttctgatttg cacttcaaaa
     1381 actcttcctt ccggaccctc cactgcagta tggagcgact gatagccatt ggattttgga
     1441 ttgataatgt aatcatcaaa ctctgctgga atttgcttcc acaaagtatg aaccatgcct
     1501 aacacttcat aacattgtgc ctcggtatct aacagaatac gaacagctgt tgcatcatat
     1561 atctcgtcta atgatacatt tttgcgcttc atctttttat aaatactatg gatatgtttt
     1621 gaacggccat aaacggcaaa gtgacgagct cctgttgctc gaatctgatg ctcaagctgt
     1681 tcgacaatgc gattgacaaa gctgtctctt tccaatcgtt tagccttcaa tcccttggca
     1741 atcgccttat attcttctgg gtgcaaatgc cggaaagcca aatcttccat ttcccacttg
     1801 attgccccta ttcccaagcg gttagctaga ggtgcgtaaa tttccatggc ttcggttgcc
     1861 aattgcttac gtaccgtttc agacagatgc cctgctgttc ttaaaataca taaacactct
     1921 gccagcttga ttaaaacaac tcgcacatca tcaaccatcg caagaagcat tttgcgaata
     1981 ttgtcaattt gatttttgtt ttgcgggtat ttatttaaag cctggaaatt gttcatggca
     2041 ctcatttttt ctatgccttt gaccaattta gcgatattgt gccccaattg ctcttcaaca
     2101 tcgtcaatag ataaatccgc ataatgaaca ttttcaaaaa taatagctgc ggccagagtt
     2161 tcctgatcta cttccaggtc agcaagcaaa tcagccattg ataacccttg ttgtaaacag
     2221 gtttgccctg tttcagtagc atgatcctga ccagccaatt gacttaaagt acaggcagct
     2281 cttacgagtt cgagattatc caaatatcct tttgaaccaa gatgatgtaa ccacatctct
     2341 acgtcaatac tgccatctgg cgtcaacgga gtcgtatctt ttactcttac catacatatt
     2401 atcctttttg aaacaaggca atagactcga catgcgctgt atgaggaaac atatccatta
     2461 ccccagctgt tatcaaaacg taaccttttt gattaaccaa tatatctgta tctcgagcta
     2521 atgttatcgg gttacaagat acatatacta tacgctcagg atcaattgca tcaatttgtt
     2581 ttacaatttc aagcgcacca gaccggggag gatcgattaa aaccttgcta aaagacgtat
     2641 taaccagatt cctaacttcc atcacatcat caagattagc agcataaaaa tccacattgg
     2701 taatatgatt agacttggca ttcatatagg ctctttcaac catgttccta ttgccttcga
     2761 caccgataac tcgagagcaa tgtttagcca tgggaagcga aaaattaccc agtccacaaa
     2821 ataagtctag cacgatatca gaatttttta attccattaa ctgaattgcc tgctttacca
     2881 tctttctgtt taactcggcg tttacctgag taaaatcgtt gggatgaaat tgaaaagtta
     2941 tttgataatc gggcagctca taattcaaat aagcatgagc atctgaagga taaaaacaaa
     3001 aaaccgaatc aagtccacct ggctgcagga atatcttgta ttggaattgt tgagcaaatt
     3061 tcctgatttt caactcatct tgctctgtta atggggttag attgcggaaa atcagcgcca
     3121 cttcattatc acctgcagcc acttcaattt gtgcgataca ctgcttatct tccatcgtat
     3181 cgattaattt tctcaaatgg acaatatcag tatctatttt tgaattaaga ataggacatt
     3241 gatttatttc tgtaatgaac cttggattat tcctctctct aaatccaacc atggtgcttt
     3301 gtttcttttc tacaaagcga gtacttaagc gtgctttatt tctataattc caaggatggc
     3361 ttgttaaagg tgacaatact gtttgaggtg cagtatggcc ataccgagat agtaaatcca
     3421 aaagatggga ttgtttgaaa cgaatttgtt cctcggcact catatgttgc aaagaacagc
     3481 ctccacacat ttgataatga gggcattttg gttcaaccct gagagtcgag ggctcgacaa
     3541 ttgataataa tttaccttca tcaaaatcct tttttacccg tgtatactga aattcaacca
     3601 cctcaccggg taatgctccc tgaataaaag ttgctttgcc attaattcgt gctatccccc
     3661 ttccatcatg gcttaaattt acgatccgag cggtttgtga ggttaaattc aattttggct
     3721 taacttttct catgaaatga ttctcaaata attatcaatt acaaaattct catgctacat
     3781 ttttagaatg ttgtatggat ataaaaaaca aaaaatgccc aaataaaggg ccgaataatc
     3841 aagggaatgc ataatgttga agaatatcac acgagctttg tctttgtcat tgttaataag
     3901 ccataacacc tgcgccaatg aagcatcttc tatcgataca gtatctcaac caacaaccgt
     3961 tcaattttta gaaaaatttg gtgcaggatt tgccaatctg gaacataaaa atctggggga
     4021 tagagttcaa ctttatttgc caggccataa cagcaagact ggtattgtaa aactgagatt
     4081 acccaatggg agtaaactaa gttatggcga gatcgtgatg tacggtggtg atatgtttgg
     4141 aaatccggca aaacccatat ccaattgctc tgcgaaaaac aggttagctt gtttccaggc
     4201 gcagtttgac gctctgggga ctttaggtaa caagcaagac aagcgttgct ctaacccact
     4261 taatcagata acccatcttg atcggttttt taccaatttg gctgaagaga tcgaaaaatc
     4321 aaaacaaaac ggaacaaatg attgggatta ttataatcag cacgactcgg ctattagcaa
     4381 ggaattaaac aaattgactt gtggtggaag tataatcagt ggttttatac cctttggcaa
     4441 ttatattaaa ttagctgaag tcaactatga tcactttgtt cctgattcac ttgtagccta
     4501 taaaacaggc catagatttg ctttggaaac agcattaaaa gcttatgaga aaaagcagca
     4561 gggacttatc gatgaagctc acaagctcct tgaattagcc tatgctcaaa acgcctttgc
     4621 aaatcattac ttaactgatt ctttttctgc agggcacatg cgcacaccaa gacgagccat
     4681 aggacatgat atattactgc cttctgtttt aaatcttctt atcgccaatt tgatgcataa
     4741 tgaagataat caacatgggt taaatgtagt gaatcatgaa ggaataagtt ggcttgccta
     4801 tggagatggc tatttataca aaccagaagc cgaattacaa agagtgatca tcttagacgc
     4861 catgcaacgt tctgcagaca gtgtccatag tacttttatg aatggagtta tccctgaaca
     4921 ctatccggag atggatgtgt ttcccgacta ctcaaaaatt gaacagataa atgatacagc
     4981 tccattattt aaagttgaaa acggcgtttt gcttaaacga gttaataata aggatcacta
     5041 tgatcatcat tggacaaaat actggagcgg gcttgtgact ttaatagagt ttcaggttaa
     5101 taaaacttaa acgttctaac ttccataaaa caggcattat gaagaaaaat attattatgt
     5161 tcattgtatc atgcgccatg tttatggagg ctattgatac aactattctc aacaccgcaa
     5221 tcccggtgat ggctcatagt ttagaagtca atccaattga tctcaaactt gcgctcatta
     5281 gctatctttt aagcttggca atctttattc ctataagtgg atggattgct gataaatatg
     5341 gtgtaaaaaa agtatttata gcggctattg gtgtctttac tctaagttcc ttatggtgcg
     5401 gattcactca aagtctttgg gaattggtat tagccagaat tatacagggt ttaggcggct
     5461 cactgaccgt gcctgttgga cgtttaatta ttctgagaac ttgtaagcgt cacgaattaa
     5521 taacaaaaat gagtattgtc gtcatggttg cctcactagg tatgctactt ggccccttac
     5581 tgggcggtat cattactgat tatttctcat ggcggtggat attttgggtt aatatccctg
     5641 ttggcataat cgccatgctt ttatctcaca aactgcttcc aactatcctg cccagatcag
     5701 ttccaccttt ggataaatca ggatttataa tgtttggagc cggcctggcc ttcttaactt
     5761 tgggtttgtc tctgtttagt gagactaaaa cggatatcag ctactctgtt tcaattatcc
     5821 ttttatctgc cttgctgtta attggatata ccagacattc gcgtaacaag agtaatccta
     5881 taataaaag
//
LOCUS       DBNCWK010000158         5781 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.158, whole genome shotgun sequence.
ACCESSION   DBNCWK010000158 DBNCWK010000000
VERSION     DBNCWK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.158"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            98..385
                     /locus_tag="AC06IO_002594"
     CDS             98..385
                     /locus_tag="AC06IO_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9875762.1"
                     /translation="MSAVMQQVTQGNDALSHQVISAVKGYLTSVGSKDANLNLYQLIV
                     EEVEAPLFRTVMELTRYNQSKAARVLGVSRGTLRTKLKRYFDDEFIGTRDF"
     gene            496..868
                     /gene="rnpB"
                     /locus_tag="AC06IO_002595"
     ncRNA           496..868
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC06IO_002595"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(957..1745)
                     /locus_tag="AC06IO_002596"
     CDS             complement(957..1745)
                     /locus_tag="AC06IO_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIO9875763.1"
                     /translation="MKKLIYISLIFVISNAFAQPAATVLFAKQKVISLREGKEYSLSR
                     GSQLEPGDTVVTGAGALINIKYKNGTLVNIGENSRYKILAFSPNPSDVQIKAELTQGK
                     IKFKTTGKSNESLKTPVIAMAITGTSAEIFVKSDKQTYLNLLEGNINNYAPGSYIATP
                     QGVVAGAFPAEGQVNTPAGTDGSISDAETTDTISSESADATDTADTTDTADAADTADS
                     AQEASDAVGFIESAQVVDQATTDSIEEEIASEIAEISLECISVI"
     gene            complement(1765..2229)
                     /locus_tag="AC06IO_002597"
     CDS             complement(1765..2229)
                     /locus_tag="AC06IO_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875764.1"
                     /translation="MKTIRLFMLSMIAHFLLTCSVIHNVWAEKTLNPEKLANTTITLI
                     SNNQNASIANCSLQSVTSCTILLHLANPGTPGILHVTNHSSFVTATNIEAVLPSSLEF
                     LVDMIESGCDVVPPGSTCSIYFYPVSGPISPQPVQLIGDNIRTATFYLGIEM"
     gene            complement(2269..4515)
                     /locus_tag="AC06IO_002598"
     CDS             complement(2269..4515)
                     /locus_tag="AC06IO_002598"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIO9875765.1"
                     /translation="MNKLTLYFSQFLNHTKWTEFFLGLTVTLLVLLTLFFNIPPISSL
                     ISQFEGRIYDQIINLNWHSKPENLKVIIIDIDEKSLEKEGRWPWPRNKIAELLNKLQE
                     NGVVTVGFDIVMSEAETNYALGLIEKLSQFSGKMTTDQKQLIATLNNVANQVDNDQML
                     VNALGKNNAILGFFFHNSEGIRKGALPTPLSDTDGNPLLANKLSVYQFTNFNGNLAAF
                     TAAATSGGFVTNLPDKDGVVRHALLLANYKNNLYPSLSLAIAMNYLLTDKVALKKYNN
                     QLTGIELDGLQIPTNRKGQILIPFWGGPGTLNYYSATDILNNQFNPEELAGSIALVGS
                     SITLLSDLHHTPVAQLFPGVEIVGNIVKGLVEQKIPREFDWESLQGKLCLVIFGLLFA
                     LLFSSIGIFGKLLIFLITTIAILTNTVILFVFYNNYIPPAFLLTITTLQTFVNFSYSY
                     VIEKRQKRRISELFGQYVPKEYVKELIEHPEHHTMEGLNREMTVLFTDIRNFTTISET
                     LGAGGVKLLLNSFFTPMTKIIFEHRGTIDKYVGDMIVAFWGAPIEDKEHASHAVLCSL
                     SCIEHLPQINTHLLEHGLPQVNIGVGIATGPMNVGDMGSEFRRAYTVLGDTVNLASRL
                     ESLTKFYQVNILVSDKTRSHLDSILWRTVDKVAVKGRKSPLTIYQPLGIVEQASARLL
                     QEQEKYETALAHYYNQDWLVAEILFNELKNVNPKIYLYQMYLERIAAFKKRGPSPDWD
                     GVYVHRSK"
     gene            complement(4714..5703)
                     /locus_tag="AC06IO_002599"
     CDS             complement(4714..5703)
                     /locus_tag="AC06IO_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HIO9875766.1"
                     /translation="MKIKFLLSMIGLILSTQSAYAKIPDSSASKTQNINDNKSAKNTN
                     NFLKKITPEFAYSYIDFNFDSTAGLNFNRYNGHSNLYSIGADHFSPVPSIIAGLYVLN
                     IDTEVSSQFQLFPNTPIPSNQTIHNNTLFAHMRKIFTPKFDIDIAGGYGQNRINSQTL
                     LNRAIADIAFSKHTNDNWLASINAVYRKRGKKISLRTYVGMLYSQINSGSYILAFQSN
                     QRGQIIAPLTTKTTYIMEGTEISYKLNSTLTPFVNGGLIQIADFTNSRPILTQQINGS
                     LPQLNMDKNGYKLGAGLIFNYKQMTLRLEEKYYSAGNIFESYQTTAGLEYRFN"
BASE COUNT         1700 a         1047 c         1149 g         1885 t
ORIGIN      
        1 ctttgggata tttgatatta tatcggtcat ggtgttagag ccaaattaat gtgctggaca
       61 tgatttatgt tcattaaatt tgtattagga gattaaaatg agtgcagtta tgcaacaagt
      121 gacacaaggt aacgatgcct tgtcacatca agtgattagt gcagttaaag gttatttaac
      181 aagtgtcggt agtaaagatg ctaatttaaa cttatatcaa ttgattgttg aggaagtaga
      241 agccccttta ttccggactg ttatggaatt aactcgctat aatcaatcaa aagctgctcg
      301 agtgcttggt gtaagtcgtg gtactttgcg cactaaatta aagcgttatt ttgatgatga
      361 attcattggt actcgtgatt tttaattcag ttatatagct aatactttcc aagttcagtt
      421 atttttcgcg gtattctaat agaaattcat cgttaattat ggcctttgcc tgggcactct
      481 gctaatattg aagtcagagt cggtcaggca atcgctcttt gcctaacagc aaggggagga
      541 aagtccgggc tccatagggc agagtgccag gtaactcctg ggaggtgtga gcctacggaa
      601 agtgccacag aaaatatacc gcccactttt cctttgggaa tcgtaggtaa gggtgaaatg
      661 gtgcggtaag agcgcaccgc gcgattggta acagtcgtgg cagggaaaac cccactcgga
      721 gcaagaccaa ataggaatcc atttgactgc aaggtcctaa cgtatggccc atacgggatt
      781 cgggtaggtc gcttgaggca tgtggtgacg catgtcccag atgaatggtt gcccacgaca
      841 gaacccggct tatcgaccga ctctaatcca tattctatgt taatgcatac cattatatgc
      901 ctttggcccc tatataaata agggggtaaa gtcgcataca aaaatgacat gccaagctat
      961 ataacactta tacactccag ggagatctcg gctatttcac ttgcaatttc ttcttctatt
     1021 gaatcagtag tcgcttgatc cactacttga gctgactcaa taaatccaac agcatcagac
     1081 gcctcttgag cgctatcggc ggtatccgct gcgtcagccg tgtctgtagt atctgctgta
     1141 tctgtagcat ctgcagactc tgaggagata gtatcagtgg tttcagcatc agagatgctt
     1201 ccatccgtgc cagctggagt gtttacctgg ccctcagcag gaaaggcacc tgctacaacc
     1261 ccctgtggcg tagcaatgta actcccagga gcataattat ttatatttcc ttctagcaaa
     1321 tttaaataag tttgtttatc agattttaca aatatctcgg cagatgtacc tgtaattgcc
     1381 attgctatca ctggagtttt cagactttca tttgatttgc ctgtggtttt aaattttatt
     1441 tttccttgag ttaactcagc tttaatttga acatcagatg gatttgggga aaaggctaga
     1501 attttatatc tggagttctc acctatattt accaaggtcc catttttata tttgatgtta
     1561 atcagggcgc ctgcccccgt aacaacagtg tctcctggct ctaattgaga gcctcgagag
     1621 agtgagtatt ctttaccttc tctgagtgag atgacttttt gtttggcaaa taatactgtg
     1681 gctgctggtt gagcaaacgc attgcttata acaaaaatta gactaatata aattaatttt
     1741 ttcatgagtt cttcctactg gatatcacat ctctatacca agataaaatg tcgcagttct
     1801 tatattgtcg ccaattaact gaactggttg cggagaaata gggccactta caggatagaa
     1861 atagattgag caggtacttc caggtggaac tacatcacaa ccgctttcaa tcatatccac
     1921 aagaaactct agtgagcttg ggagtacagc ttcaatattg gttgctgtaa caaaagaaga
     1981 gtggttagtc acatggagaa ttcctggggt accagggtta gcaagatgaa gaagtatggt
     2041 acaacttgtg actgattgaa gtgagcaatt ggcaattgag gcattttgat tattagaaat
     2101 taatgtgatt gtggtatttg ccagtttttc gggatttagg gttttttcag cccaaacgtt
     2161 atgaattact gagcaggtta ataaaaaatg tgcaatcatt gataacataa acagcctaat
     2221 tgttttcata atccttccta ttgcagtatt taaaatttct tcttgatctc acttgcttct
     2281 atgaacatac accccatccc agtcaggtga aggccctctt tttttgaatg ccgctattct
     2341 ttccaaatac atttggtaca aataaatttt agggtttaca ttttttaact cgttgaagag
     2401 aatctcagcg acaagccaat cctgattgta atagtgagct aatgccgttt cgtatttttc
     2461 ctgctcctgc agaagtcttg cagaggcttg ttcaacaata cctagaggtt ggtaaatagt
     2521 caatggactt ttacgacctt tgaccgccac tttgtctacg gtacgccata atatgctatc
     2581 caggtgagag cgagttttgt cacttaccag gatattcact tgatagaatt tggttagact
     2641 ttctagcctg gatgcgaggt taacggtatc gccaagaaca gtataggctc gacgaaactc
     2701 agaacccatg tcaccgacgt tcataggacc agttgctata cctactccaa tattaacttg
     2761 tggcagtcca tgttccaata aatgggtatt aatttgaggc aagtgctcaa tgcaggaaag
     2821 agaacacaaa acggcgtgac ttgcatgctc cttgtcttct attggtgctc cccaaaatgc
     2881 tactatcatg tccccaacgt atttatcaat ggttcctcta tgctcaaaaa tgattttggt
     2941 cattggggtg aaaaatgaat ttaacaacag ttttacaccg cctgcaccaa gtgtttcgct
     3001 gattgttgtg aaattacgta tgtctgtaaa cagaactgtc atttcacgat tcaacccttc
     3061 catagtgtgg tgttcgggat gctctattaa ttcttttacg tattctttgg gtacgtattg
     3121 accgaacaat tcacttattc gtcttttttg cctcttttca atgacatatg aataggaaaa
     3181 attaacaaaa gtttgcaagg tagtgatggt taataaaaat gcagggggga tgtaattgtt
     3241 gtaaaatacg aacaggataa ctgtatttgt taatattgct atggttgtta ttaaaaagat
     3301 taataatttg ccaaaaatac caatagaaga aaataaaagc gcaaataata agccaaaaat
     3361 cactaaacaa agttttcctt gcaatgattc ccagtcaaat tctcggggaa ttttttgttc
     3421 caccagtcct tttacgatat tacccactat ttcaacccca ggaaataatt gggcaactgg
     3481 tgtatggtgt aaatcagata agagtgttat ggatgagcca actaaggcta tactcccagc
     3541 caattcctcg ggattgaatt ggttgttcaa tatgtcagtc gctgaatagt aatttagtgt
     3601 tcctgggcct ccccaaaatg gaataagaat ttgacctttt ctatttgtgg ggatttgtag
     3661 cccatctaat tctatacctg tcaattggtt gttatatttt ttcagagcaa ccttgtcagt
     3721 taagaggtaa ttcattgcta ttgccaaaga taaactggga tagaggttgt ttttataatt
     3781 ggccaataaa agtgcatgtc ttacaacccc atctttgtcg ggtaaatttg taacaaaccc
     3841 gccagaagtt gcagccgcag taaatgcggc caaattaccg ttgaaattag taaattgata
     3901 cactgacagc ttatttgcca ggaggggatt gccgtcagta tcacttaatg gtgtcggtaa
     3961 tgcccctttt ctaatgcctt cgctattatg aaaaaaaaaa ccaagtattg cattattttt
     4021 gcctaaagcg ttaacaagca tttgatcatt atcaacctga tttgcaacgt tgtttaatgt
     4081 ggcaatcaac tgtttttgat cagttgtcat ttttccagaa aattgactca gtttttctat
     4141 gaggcccaaa gcataattcg tttccgcttc tgacatgact atatcgaaac caacagtgac
     4201 cacaccattt tcttgtaatt tatttaataa ttcagctatt ttatttcttg gccatggcca
     4261 tcttccttct ttttctaatg atttctcatc aatgtctatg ataataactt tcaggttttc
     4321 aggttttgaa tgccaattaa ggttgattat ttgatcataa attcttcctt caaattggga
     4381 tattagtgaa ctaatcggtg gaatattaaa gaacaaggtt agcaatacca aaagggttac
     4441 tgtcagcccc aagaaaaact cagtccactt tgtgtgattg aggaattgag agaaataaag
     4501 agtcaattta ttcacaaaaa ttatcctttt gctactctta aatacaagat gaaagagcat
     4561 cctctttatt aatcagtata tagtctgaga ggaaagggca gaaataatgt ttttcagcct
     4621 tgtagatcac aggtgtgttt gctccatgtt attacaaaat tcattttcgc gcttctggta
     4681 tgaagaacaa taaataaact actataaaac taactaatta aatcggtact ccagtccggc
     4741 agttgtttga taactttcaa aaatgtttcc tgcgctgtaa tatttttctt caagccttaa
     4801 agtcatttgt ttataattaa aaataagtcc ggcaccaagt ttgtagccat tcttatccat
     4861 attgagctga ggcagagagc cattaatttg ttgagtaagt ataggtcgac tattggtgaa
     4921 gtccgctatt tgaatcaaac caccgttaac gaaaggggtt aatgttgaat tgagtttgta
     4981 actgatttca gtcccttcca taatataggt tgtttttgta gttaaaggag ctatgatttg
     5041 cccgcgttgg ttggactgga aggctaatat ataactaccc gaattaattt ggctgtataa
     5101 cattcccaca taggttctta gagatatttt tttccctcgt tttctataaa ctgcattgat
     5161 gctggcaagc cagttgtcat tagtatgttt tgaaaaagcg atatcagcga tggcacgatt
     5221 taacaaagtt tgcgagttta ttctgttttg accatatcct ccagcgatat ctatatcaaa
     5281 ttttggggta aatattttac gcatatgtgc gaataatgtg ttgttgtgaa tcgtttgatt
     5341 ggaaggaatt ggagtattgg gaaacaattg aaattgagaa ctcacttcag tatctatgtt
     5401 tagaacataa agccctgcaa tgatgctcgg tactggagaa aaatgatcag cgcctatgga
     5461 ataaagattg gaatgcccat tgtatcgatt aaaattaaga ccagctgtcg aatcaaagtt
     5521 aaaatctata tagctgtatg caaattcagg cgtgattttc tttaaaaagt tattagtatt
     5581 ttttgcgctc ttattgtcat ttatattttg tgtttttgat gctgagctat caggaatttt
     5641 agcataggct gattgtgtgc tcagtattaa tccaatcatt gataacaaaa actttatttt
     5701 cattatttca aaaatcctta gcgaaatgac aaattaatta gcttcaaata ataccgggaa
     5761 aaaagtcata tatgcaatta a
//
LOCUS       DBNCWK010000159         5657 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.160, whole genome shotgun sequence.
ACCESSION   DBNCWK010000159 DBNCWK010000000
VERSION     DBNCWK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.160"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            98..754
                     /gene="adk"
                     /locus_tag="AC06IO_002600"
     CDS             98..754
                     /gene="adk"
                     /locus_tag="AC06IO_002600"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIO9875767.1"
                     /translation="MRLMLLGGPGAGKGTQASLLINRYKIPQISTGDMLRAAIAKGTP
                     LGLSAQKIMESGGLVSDDIIIGLVKERLKNPDCDRGFLFDGFPRTLVQAEALKDAEIH
                     LDHVIEIAVDDEEIIERISGRRIHQPSGRVYHVVNQPPKNPGVDDITGEPLIQRDDDK
                     EETIRKRLQVYHSQTAPLVQYYKEWAESGSKEAPKFHTISGTGTVDQIFDNIVTILET
                     "
     gene            763..1125
                     /locus_tag="AC06IO_002601"
     CDS             763..1125
                     /locus_tag="AC06IO_002601"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIO9875768.1"
                     /translation="MPTQTINSAEFESLINDNEIVFIDFWAEWCAPCKQFSKVYEQVS
                     DQFPAIKFVKVNIEEQQELADFFEIRSIPHLMVFKDSIVIYSDAGSIPESTLKELAQQ
                     ALDADVSEIRAQLEQKDK"
     gene            complement(1085..2605)
                     /gene="glpD"
                     /locus_tag="AC06IO_002602"
     CDS             complement(1085..2605)
                     /gene="glpD"
                     /locus_tag="AC06IO_002602"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIO9875769.1"
                     /translation="MDQVFDVAIIGGGINGCGCAADAALRGLSVILFEQDDLASKTSS
                     SSTKLIHGGLRYLEHYEFGLVKKALQERQTLLNLAPHLVHSQSFVLPYLKHMRPSWLL
                     RLGLFFYDHLSRKNHLPKSRSIHRNNKNNYFTPLKEELNRGFLFYDATTDDARLTILN
                     AIQAKNHGASIRPHAKVTHAEVINNIWHLTIQPTKGQSYKAYAKSIINATGPWVQSIA
                     QLTHTPIQKDITLVKGSHIIVPKLFEGNHAYFLQHNDQRVIFVIPYHGFSMIGTTDIH
                     YTGDLNHIEIGEEEIDYLIELVNAYFKFKITKEDVVHSWSGIRPLLADDSKEVKSLSR
                     DYSYEFNHSPAPIITIFGGKITTYRQLAEEVVDQLTPVFPQMKPSKTQHTPLPGATLA
                     DMSFTQYVNYAKTKYHWLDDELLNRYLNTYGCYTEIFLSQCNNMEAMGKKYGPSLYQV
                     EVDYLILEEWANYCDDILNRRTKLGLTMDNASKKELADYLASISTYPSAQAELVFH"
     gene            2667..4142
                     /gene="glpK"
                     /locus_tag="AC06IO_002603"
     CDS             2667..4142
                     /gene="glpK"
                     /locus_tag="AC06IO_002603"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIO9875770.1"
                     /translation="MNYILAIDQGTSSTRAMIYTLQGDLIANSQYPLTQYYPNSGWVE
                     HDPEEIWQKTLNAMRDVVSQVQGKAILCCGLTNQRETTLIWNKKTGECLAPAIVWQDR
                     RTEEFCYSLSEYSSMIQEKTGLLPDPYFSASKLNWLLKNNAQAQSLINQGDLAFGTID
                     SFLIWRMTKGASHATDITNASRTMLFNIHDEKWDKQLLELFSISESVLPAVFSSDNHF
                     GMIDKEWLGVEIPITGVAGDQQAALIGQGCFNGGMVKATFGTGGFLLLNTGDKPVFSK
                     HKLLTTIAYKIKEKTVYGLEGSIYHAGTTVKWLRDEMKLISHSAETEVLARSLDSNNG
                     VYLVSSFTGLGAPHWLSITGTAIIGVSRSTNRAHFARAALEGVCYQTRDVLTCMRDDS
                     QLDLTLLRADGGMAVNQWFLQFMADQCQLTIQKPKDIETTAKGAAVLAAIGSGLFDSV
                     SSVNKVWDIENEFQPFREREVVEKDFRGWLEALAMVKARHT"
     gene            complement(4236..5507)
                     /gene="gltA"
                     /locus_tag="AC06IO_002604"
     CDS             complement(4236..5507)
                     /gene="gltA"
                     /locus_tag="AC06IO_002604"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIO9875771.1"
                     /translation="MTQKTAKLSIDGQETIDLPIYTPTLGNDVIDITQLGSHGVFTFD
                     QGFLSTASCESKITYIDGDKGILLYRGYPIEQLAEKKDFLDVSYLLLNGELPNKEEKK
                     AFVSLINNHTMVHQQMYQFLNGFRRDAHPMAIMVGIVGALSAFYHESMDLNNAQDRFI
                     SAIRLVAKMPTLAAMSYKYSIGMPYMYPKNKMSYAENFLHMMFGVPSEDTTPDPVLVR
                     AMDTIFILHADHEQNASTSTVRLAGSTGANPFACISAGIGALWGPAHGGANEACLNML
                     KEIGDVSRIDHYIKRAKDKNDPFRLMGFGHRVYKSYDPRAKVMRKTCYDVLEAVGAKD
                     APLFKLAMELERIALEDEYFIEKKLYPNVDFYSGLTLSAIGIPTNMFTVIFALARTVG
                     WMSHWMEMVATKSRIGRPRQLYTGEKIRDVK"
BASE COUNT         1740 a         1031 c         1161 g         1725 t
ORIGIN      
        1 tgtcaatgtg cactaaataa ttataaaatt ttatagtgct actatgttac tatatttgaa
       61 agtatataat gattacgaat tatttaagga gttttggatg cgattaatgc ttttaggtgg
      121 accgggagcc ggcaaaggaa cccaagcttc acttttaatt aaccgttata aaattcctca
      181 aatttctacc ggtgacatgc tgcgtgctgc catagcaaaa ggtacgccct taggattaag
      241 tgcccaaaaa atcatggaat caggtggctt ggtgtctgat gatattatta tcgggttagt
      301 gaaggaaaga ctaaaaaacc ctgattgtga tagaggattc ctatttgatg gttttccaag
      361 aacattggtt caggccgaag ctttaaaaga tgcagaaatc catttggatc atgtgataga
      421 aatagccgtc gatgacgaag aaataattga acgcatcagc gggcgacgca ttcaccagcc
      481 ttctggtagg gtttatcacg ttgtaaatca accaccgaaa aatccgggtg tagatgacat
      541 cacaggtgag cctctgattc aacgtgatga tgataaagaa gaaaccatac gtaagcgctt
      601 acaggtttac catagccaaa cagcgccatt ggtgcaatat tataaggaat gggctgaatc
      661 tggttctaaa gaagctccaa aatttcacac tattagtggt acaggtacag tagatcaaat
      721 atttgacaac atcgtaacga tactcgaaac ttaggagtta acatgccgac ccagactatt
      781 aacagcgctg aatttgaatc attaattaat gacaatgaga tagtttttat tgatttctgg
      841 gctgagtggt gtgctccctg taagcaattt tcaaaagtat atgagcaagt ttccgatcaa
      901 tttccagcta ttaaatttgt taaagttaac attgaagagc aacaagagtt agcagatttt
      961 ttcgagatcc gatccatccc tcatcttatg gttttcaaag acagtattgt tatttattct
     1021 gatgctggaa gcatacccga gtccacttta aaagaattgg cgcagcaagc acttgatgct
     1081 gatgttagtg aaatacgagc tcagcttgag cagaaggata agtagatata ctagccagat
     1141 aatccgcaag ttctttttta ctggcattat ccatagtaag gccgagcttg gtgcggcggt
     1201 taagtatgtc atcacaataa ttcgcccact cttctaaaat aagataatct acttccacct
     1261 ggtagagtga tgggccatat tttttcccca ttgcttccat attgttgcat tgcgaaagaa
     1321 aaatttcagt ataacaacca taggtattga ggtagcgatt taatagttca tcatccagcc
     1381 aatgatattt tgttttggcg taattgacat attgagtaaa actcatatcc gctaaagttg
     1441 cccccggtaa gggcgtatgt tgtgttttcg atggcttcat ctgtggaaat acaggggtaa
     1501 gttgatctac aacttcttca gccaactgtc tgtaagtggt tatttttcct ccaaaaattg
     1561 taataatggg agctggggaa tggttaaact cataggaata gtcacgactt aatgatttga
     1621 cttctttgct atcatcagct aataaaggcc ttatgccact ccaggaatgg actacatctt
     1681 ctttagttat tttaaacttg aagtaggcat ttaccaattc aattaagtaa tcaatttctt
     1741 cttcaccaat ctctatgtgg ttaagatccc cagtatagtg gatatcggtg gttccaatca
     1801 tactaaagcc atgatagggg ataacaaaaa taacccgctg atcattatgt tgcaagaaat
     1861 aggcatgatt tccctcaaaa agtttgggga cgataatatg gctaccttta accagggtaa
     1921 tatctttttg gataggtgta tgagttaatt gggcaatgga ttgcacccat ggtcctgtgg
     1981 cattaatgat gcttttagca taagctttat agctctgccc tttggtgggc tggatagtta
     2041 aatgccatat gttattaatt acctcagcat gggttacttt ggcatgtggc cttatgcttg
     2101 cgccatgatt tttggcttgt atggcattaa gaatggttaa acgagcatca tccgtggtgg
     2161 catcatagaa aagaaaaccg cgatttagct cctcttttag tggcgtaaaa taattattct
     2221 tattatttct gtggatcgat ctggatttgg gtaaatgatt ttttcgactg agatgatcgt
     2281 aaaaaaataa acctaacctc aataaccatg aggggcgcat atgttttaag tagggcaaaa
     2341 caaaagattg ggaatggact aaatggggag ccaaattgag aagcgtttga cgttcctgga
     2401 gtgctttttt gactaaacca aactcataat gctctaaata tcttaaacca ccatgaatta
     2461 attttgtgct acttgatgag gttttcgaag ccagatcgtc ctgttcaaag agtatcacag
     2521 acagacctct taatgctgca tctgcagcgc agccgcagcc attgatgcct ccaccaataa
     2581 ttgcaacatc aaaaacctga tccatgtttt tatctccgaa aaaagattac actcaatata
     2641 tataaatata gagggatttt ataataatga actatatact agccattgac cagggaacaa
     2701 gtagtactcg cgctatgatc tacacccttc aaggtgattt aatcgcaaat agtcagtatc
     2761 ctcttactca atattacccc aattcgggtt gggtagaaca cgatcctgag gaaatatggc
     2821 aaaaaactct gaatgcgatg agggatgttg tatctcaagt tcaaggaaaa gcaattttat
     2881 gctgtggcct taccaaccaa cgggaaacta ctctgatttg gaacaaaaaa acaggggaat
     2941 gcctcgcgcc agctattgtg tggcaagata gacgtactga ggagttttgt tattccttgt
     3001 ctgaatactc gtcaatgata caagagaaaa ccggattact tcctgatccc tatttttcag
     3061 cgagcaagtt aaactggtta ctaaagaata atgctcaagc tcaatctcta attaaccagg
     3121 gggatttggc atttggaaca atagacagtt ttttaatatg gcgcatgact aaaggtgcaa
     3181 gtcatgctac cgatatcacc aacgcatcca gaactatgct gtttaatatc catgatgaaa
     3241 aatgggataa gcagttgctg gagctttttt ctatttcaga atcggtatta ccagcggtat
     3301 tctccagtga taatcatttt gggatgatag ataaagaatg gctgggagta gaaataccaa
     3361 ttaccggggt tgcaggtgat caacaagccg ctttaattgg gcaaggttgt tttaatggag
     3421 gaatggttaa agctactttt ggtacggggg gatttttatt acttaataca ggtgataaac
     3481 cagttttctc aaagcataaa ttgttgacta ctattgccta taaaattaaa gaaaaaacag
     3541 tatatggttt ggagggtagt atttatcatg ccggaactac tgttaaatgg ttacgcgatg
     3601 aaatgaagtt gatatctcat tctgctgaaa ctgaagtatt ggctcgaagt cttgatagca
     3661 ataacggcgt atatcttgtt tcatctttta ctggcctggg agcacctcat tggttatcaa
     3721 taacaggaac agctattata ggtgtatccc gttcgacgaa tagagcacat tttgccagag
     3781 ccgccttgga aggagtttgt tatcaaacac gtgatgtatt aacctgtatg agagatgata
     3841 gtcaattgga tttgacttta ttaagagcgg atggagggat ggcagtaaac caatggtttc
     3901 tacaatttat ggctgatcag tgccagttga caattcaaaa acccaaagac attgaaacga
     3961 ctgccaaagg ggcggcagta ttagccgcaa taggttctgg actatttgat tcagtttctt
     4021 cagtaaataa ggtatgggac attgagaacg agtttcagcc attcagggaa agagaggtag
     4081 ttgaaaaaga ttttcgtggc tggctagagg cgttagcaat ggttaaagca aggcatacat
     4141 aggctggtct tatatttaaa ttggtgcgat taaattagat tgcagtagct cgcattatag
     4201 tttaatacaa gctactgatt aacacattgg attttttatt ttacgtcacg aattttttca
     4261 ccagtataca attggcgagg tctgccaatg cgtgacttgg tggctaccat ttccatccag
     4321 tgactcatcc atccaacagt gcgagctaaa gcaaatatca ctgtgaacat attggttggt
     4381 attccaatag cgcttaaagt caatcctgag taaaagtcta cattcgggta gagttttttc
     4441 tcaatgaaat attcatcttc aagagcaata cgttcaagtt ccattgctaa tttaaataaa
     4501 ggtgcgtctt ttgctccaac ggcttctaat acatcataac aagtcttacg cattactttt
     4561 gctcttggat catagctttt atagaccctg tgcccaaaac ccattaaacg gaatggatca
     4621 tttttatctt tggctcgttt aatgtaatga tcgatgcgac tcacatcgcc tatttccttt
     4681 agcatattta agcaggcttc attcgctcca ccatgagctg gtccccataa agcccctata
     4741 ccagctgaaa tacacgcaaa ggggttagct ccagtagagc ctgccagacg tactgtcgat
     4801 gtagatgcat tttgttcatg atcagcatgt agaataaaaa tagtatccat agctcttacc
     4861 aatactggat ctggcgtagt atcttcggat ggaacaccaa acatcatgtg aagaaaattt
     4921 tctgcataag acattttatt tttaggatac atgtagggca tgccaatgga gtatttataa
     4981 ctcattgcgg ctaaagtagg cattttagcg accagtctga ttgcggaaat aaaacggtct
     5041 tgtgcgttat tcaaatccat ggattcatga taaaaagcag acaaagcccc cacaattcca
     5101 accattatag ccattgggtg agcgtcacgt ctgaacccat ttaaaaactg gtacatttgc
     5161 tgatgaacca tagtatgatt gttgatcaga ctaacgaaag ctttcttttc ttctttgtta
     5221 ggtagttctc cattgagtaa taaataactt acatcaagaa agtccttttt ttctgccaat
     5281 tgttctatag gataacccct atacaacaaa atgcctttat ccccatcaat ataggtgatt
     5341 tttgattcac aggaggcagt tgataaaaaa ccttgatcaa aggtaaagac gccatgtgaa
     5401 ccaagctggg taatatcaat cacatcatta cctagggtgg gagtatagat tggtaaatca
     5461 atcgtctctt gcccatcgat gctgagtttc gctgtctttt gagtcattgc ttctcctgat
     5521 tatttacatg ctttaagcat tcataattgc gttagtgcgc ggaactatat aaaaatgaca
     5581 acatacagtc aaattaaacg taattttggt cattaaaaat gctagcccaa tatccaatgt
     5641 taattaaaca ttcaaat
//
LOCUS       DBNCWK010000160         5639 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.161, whole genome shotgun sequence.
ACCESSION   DBNCWK010000160 DBNCWK010000000
VERSION     DBNCWK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.161"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..359)
                     /locus_tag="AC06IO_002605"
     CDS             complement(<1..359)
                     /locus_tag="AC06IO_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIO9875772.1"
                     /translation="MKTKQEVSQQDKLKDSKSSTPLQTKEPWFISDILNITFDSYDFS
                     ISVTEQAPIPSRIVFSGGGSRILAHIGALDELTRHGLKFTEFSGSSAGAMVAAFAYLG
                     YNCSEIKQIISWFNEDKL"
     gene            717..2414
                     /locus_tag="AC06IO_002606"
     CDS             717..2414
                     /locus_tag="AC06IO_002606"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIO9875773.1"
                     /translation="MIAKSKTDMNRIQMGTNKEFPGKSHLKPSKSCGTDDSNFQVEFL
                     LDVIQKLSLAKDLETIMFIVRKAARTLTGADGSTFVLRDGNQCYYAEEDAIAPLWKGK
                     RFPMSACISGWVMEHRQSVIIEDIYSDPRIPFDAYRPTFVKSLAMVPIRTAAPLGAIG
                     IYWAEYYFPTEEQLNWLQVLADSTSVAMENVMLQSELEKGQKETTAQIEMTRKLMEVN
                     KNLECALYELNHRNKEMQWLKELSSTLQTCLYIKEAYHLIALYAAKLLPGSSGIFYVM
                     HPSRNYLEAMSSWGDSLIEERIIKPDDCLGLRRGSIYTVEEPQKDLICPHHTINSNLK
                     PYTCIPLFAQSDILGLVCMEWKSCKKEAEEEINKENNQEILASMMAEQIALGISNIML
                     RETLRNQSLRDVLTGLYNRRYLNEALDRELNRCARKSLSLAVLMLDIDHFKQFNDKFG
                     HEAGDMVIQAFANVLRQVTRKEDIACRYGGEEFILIIPEIDLEAALQRAQSIHDAVSR
                     IHLRYGGNALTQITISIGLTMYPAHGNDMQDLITAADNALYEAKNSGRNKTVLYHKN"
     gene            complement(2534..3352)
                     /gene="ceg29"
                     /locus_tag="AC06IO_002607"
     CDS             complement(2534..3352)
                     /gene="ceg29"
                     /locus_tag="AC06IO_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector Ceg29"
                     /protein_id="HIO9875774.1"
                     /translation="MTNSNHLRNPVTKSLNDLCQDELSVIGSYLGAWNDLWSLTFTSS
                     EMKKSLIHQQTDELWKFFFKEFFPYIPLPTEDFYKNFMKNYKIWYQTLYRVKQDPFFL
                     MYLDKLKEDPAHLFRNDKEIIIAAIEKQPFLYKNFSRGYKEDEEVILCALKNNRFIIH
                     DVPRTVREGFKEIADIKEDKARVDACDKLIDKLSPQRVSKIKSLDLFKSQLTLQAQNI
                     TKKSGEQVNNRQLQRSTAPITPEGISSLVHLGLFRDTQIQYEEKEESLNNKYRK"
     gene            complement(3476..3667)
                     /locus_tag="AC06IO_002608"
     CDS             complement(3476..3667)
                     /locus_tag="AC06IO_002608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875775.1"
                     /translation="MPKCNDYHVYQLLPFVVTNHQIILALLIKIWQRKKHTLCEQGKA
                     RISAALFYKIIPKSSSKKN"
     gene            complement(3677..4336)
                     /locus_tag="AC06IO_002609"
     CDS             complement(3677..4336)
                     /locus_tag="AC06IO_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875776.1"
                     /translation="MLTKNLNEALSAIKYVKSKLVYGSNTEEDSKKPNWEPGRIILLR
                     KEVHALAIVYARSHNGKAGFDIISKLTHAALLKYGFGNCGEQAQTAFCYLQSRGVAPL
                     DYCQTSIGEHCLIVIGRVSGSDPNDISTWGEDAVICDPWAEKAYPLSEFRAMQSPDND
                     IRYPEICYANGKSPKPYLAGELISHHRIEPASKASSLSFFKKENYEHSQLNSATPMLI
                     A"
     gene            complement(4663..5577)
                     /locus_tag="AC06IO_002610"
     CDS             complement(4663..5577)
                     /locus_tag="AC06IO_002610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875777.1"
                     /translation="MYYQLNLSELNGSQLSEAIKNIPGNVTYLDLRDSKLGNKTGTEL
                     AQAFAVIPASVTSLQLSWNHLDKKTGAELAQAFAAISASVTSLHLCNNMLDKKTGAEL
                     AQFLAAIPATITTINLSWTDLGNKTGAELAQAFAAIPATVTSFELRGTKLNYKTGAEL
                     AQAFAAIPATMTYVDLRGNSLYKKTDDELALISAAIPANLTVNWGFDIAQRIGNSKKE
                     SSQSQLINISMQVLGGFMAVVGCAAVATAFVFLNAATFGIPGLILAGLGVASILTGVG
                     LFATGTYSNRHEKPNMSLVDSAPCLAAG"
BASE COUNT         1663 a          997 c         1150 g         1829 t
ORIGIN      
        1 agtttatctt cattaaacca ggaaataatt tgttttattt cggaacaatt ataaccaaga
       61 taagcaaatg cagcgaccat tgcaccggct gagcttccgg aaaattcagt gaactttaat
      121 ccatgcctgg ttaactcatc cagggcacct atgtgtgcca gtattcttga acctccacca
      181 ctgaaaacaa tacgagaggg tatgggggct tgttcagtga cagagatact aaaatcatag
      241 gagtcaaaag taatatttaa gatatcagag atgaaccatg gttcttttgt ctgtagtggt
      301 gtagacgatt ttgaatcttt taatttatcc tgctgagaaa cttcttgctt tgttttcata
      361 ccgataatcc cttatataaa acaaattcta agttattttt tacgattctt ttgctacagc
      421 catcacaatc tatcgagtat ttaaaacgat ttcgccctaa ggtttttgta ttgagggttc
      481 tctccttttt taaatgattg gtgtttcagt tattgggtat tttaaatcaa attgagtagg
      541 ttaagatctc atggatcaat aattaacata aatggtcttt gagcagacgt caacttgttt
      601 caacaattat tcgaaatgaa aatttaagtt actagacaac ttatagagag gagtgatccg
      661 aaggatattt tgtttaaaaa acactcattt agtattataa ttacaaagag acagggatga
      721 tagcaaagag taaaacggac atgaatagaa tacagatggg aacaaataaa gagttcccag
      781 gcaaatcaca cctcaaaccg tcaaaatctt gtggaactga tgactccaat tttcaggttg
      841 agtttttatt ggatgtcata caaaaacttt ctcttgcaaa agatctggaa acaattatgt
      901 tcattgtacg taaggccgct cgtacattga ctggagctga cggttcgacc tttgtattgc
      961 gtgatggcaa ccaatgctat tacgcggagg aagatgcaat tgcccctctt tggaaaggga
     1021 aacgattccc tatgagtgct tgtatcagcg gttgggttat ggaacatcga cagtcggtca
     1081 ttattgaaga tatttattct gatccaagaa ttccctttga tgcttatcgg ccgacttttg
     1141 tgaagagctt ggcgatggtt cctatcagaa cagcagcccc tttgggtgcc attggaattt
     1201 attgggcaga atattacttt cctacagagg aacaattaaa ttggttgcag gtactggctg
     1261 atagcacatc tgttgcaatg gaaaatgtga tgctgcaatc agaattggag aaaggccaga
     1321 aagaaacaac tgctcaaata gaaatgacta gaaaattaat ggaggtcaat aaaaatcttg
     1381 aatgtgcctt atatgaatta aaccaccgca ataaagaaat gcaatggttg aaggagttaa
     1441 gttcaacatt gcaaacctgt ttatatatca aggaagccta tcatttaatt gctctgtatg
     1501 cggctaaatt attacctggg tcatcaggaa ttttctatgt tatgcatccc tcacgtaatt
     1561 atcttgaggc tatgagctca tggggagact ctttgataga agaaaggatc attaagccag
     1621 atgattgtct gggtttgcgt cgtggttcga tttatacagt agaagagcct caaaaggact
     1681 taatatgccc ccatcataca atcaacagta atttaaaacc ttatacctgt attcctttat
     1741 ttgctcagag cgatattctc ggtttggttt gtatggaatg gaaatcttgt aaaaaggaag
     1801 cagaggaaga aatcaataaa gaaaataatc aggaaattct cgcaagtatg atggcagagc
     1861 aaatagcgct tggtatttcc aatattatgc tgcgtgaaac tttgcgtaac caatctttac
     1921 gagatgttct cacagggctt tataatcgac gttatttgaa tgaagctctg gatcgagaat
     1981 taaaccgctg tgcaagaaaa tcattatcac ttgctgtgtt aatgttggat attgatcatt
     2041 ttaaacagtt taatgataaa ttcggacatg aagcgggtga tatggtgata caggcttttg
     2101 ctaatgtttt gcgtcaggtt acccgaaagg aggatatagc ctgccgctat ggaggggaag
     2161 agtttatcct gattatacca gagattgatc tggaagcagc cttacaaagg gctcaatcta
     2221 ttcatgatgc agtcagtcgt attcatttgc gttatggagg taatgcttta actcaaatca
     2281 caatttccat aggattaact atgtacccag cgcacggtaa cgatatgcaa gaccttatta
     2341 ccgccgccga taatgcgctt tatgaagcca aaaactcagg acgaaataaa acggtattgt
     2401 atcataaaaa ttagggtact ttgttgcaat caaaacatat tagcttccgg ccttattcaa
     2461 tctattaagg ctatgtttgg ctgttactgt aatgaatttg cgatagagca acccttatca
     2521 aaatttattt gcctcatttt cgatatttgt tgtttagaga ttcttccttt tcctcatatt
     2581 ggatttgtgt gtctctaaac aggcctaaat gaacaagaga gctgatacct tcgggtgtta
     2641 taggcgcagt tgatctttga agctggcggt tatttacttg ttcaccactt ttttttgtta
     2701 tattttgtgc ttgcagagtt aattggcttt taaacaagtc gaggcttttg attttagaaa
     2761 cacgttgtgg tgaaagcttg tcaattagtt tgtcacacgc gtcaactctt gctttatctt
     2821 ctttgatatc agctatttct ttgaatcctt ctctcactgt tcttggtaca tcatggatta
     2881 tgaatcgatt atttttaaga gcacacagaa taacctcttc gtcttctttg tatcctcggg
     2941 aaaaattttt atacaaaaag ggttgtttct caatggctgc tataataatt tctttatcat
     3001 tcctaaataa gtgtgcagga tcttctttga gtttatccag atacattaga aaaaaaggat
     3061 cctgtttcac acggtacaat gtttgatacc atattttata gtttttcatg aagtttttat
     3121 aaaaatcctc tgttggtaga ggaatataag ggaaaaattc cttgaaaaaa aatttccaca
     3181 actcatcagt ttgctgatgg attaaggatt ttttcatttc tgatgatgta aatgtcaagc
     3241 tccataaatc attccaggca ccaaggtagg agccaattac agacagctca tcttggcaaa
     3301 ggtcatttaa tgatttggta acaggattcc ttaaatggtt actgtttgtc attttttacc
     3361 tcctgattgt ggttttgttt ttctatcatg ttttttggat atgcaaatgt gacttcaaga
     3421 tgactataat gctttctttt tattaagaga atattaatag ttaacaatag atagattaat
     3481 ttttcttgct ggatgatttt ggaattattt tgtaaaagag agcagcagag atcctggctt
     3541 ttccttgctc gcacaaggtg tgtttttttc tttgccagat tttaatcaat agcgccagga
     3601 taatttgatg attcgttaca acaaaaggta gtaattggta cacatggtag tcattgcact
     3661 ttggcattgg actgctttaa gcaataagca tgggtgtagc actatttaat tgtgaatgtt
     3721 cgtaattttc ttttttgaag aaagagagcg atgatgcttt cgaggcaggc tctattctgt
     3781 gatgactgat taattcccct gccaaataag gctttggact tttaccatta gcataacata
     3841 tctcaggata gcgaatgtca ttatcgggac tttgcattgc tctaaattca gaaagaggat
     3901 aggctttctc agcccaggga tcgcaaatta cagcatcctc tccccaggtc gaaatatcat
     3961 tgggatcaga accactaacg cgcccaatca ctatcaggca gtgctctcct attgatgttt
     4021 gacaataatc aagcggcgca actcctcgag attgcagata acaaaatgct gtttgtgctt
     4081 gctcaccgca attgccgaat ccgtatttta agagtgccgc atgggttaat ttggagatga
     4141 tgtcaaatcc tgctttgcca ttatgtgaac gagcatagac gattgctagt gcatgaactt
     4201 ccttccttaa gagaataatt cttcctggtt cccaatttgg cttctttgaa tcttcctcag
     4261 tattggaacc ataaactagt ttggatttta catattttat tgcgcttaag gcttcattca
     4321 gatttttggt gagcattaaa aaaatctcag agggtaaaac agacttatta tatcaaataa
     4381 aatacatttt gttaatattt tgtttatatg ggtttgtttt tagattgtat gattttattt
     4441 tgtggagaca caagtgctaa gctcccaggc agcagttcgc tcagcctgaa ttcaaaatat
     4501 gccaaaataa ttaacagttc aattttgtcg aataccgatt tttgaggata atgatattta
     4561 aataggtgga gaatgtagat cgtagatcaa acgccagtgc tcgtagttga gaacaaccct
     4621 aaaagatgcg ttttatttct ctataccacg ggctgtttta atttaaccag ctgccaggca
     4681 aggtgctgaa tcaaccaaag acatattagg tttttcatgc ctattactgt aggtgccggt
     4741 agcaaataaa ccaactcctg taagaataga agctactcct aagcctgcca agattaatcc
     4801 cggtatacca aaagttgcag catttagaaa gacaaaagcc gtggcaactg cagcacaacc
     4861 aactactgcc ataaaaccac ctaatacctg catgctaata tttatcagtt gtgattggct
     4921 tgactctttt tttgaattac cgattctttg agcgatatcg aacccccagt taacggtcag
     4981 gttagctgga atagccgcag aaatcagcgc taattcatcg tctgtttttt tgtaaaggct
     5041 attcccgcgc aaatcaacat aggtcatagt ggcggggatt gctgcaaagg cttgggctaa
     5101 ttcagcgcct gttttgtagt taagtttagt gccgcgcaat tcaaaggagg tcacagtggc
     5161 agggattgct gcaaaggcct gagctaattc agcgcctgtt ttgttgccaa ggtcagtcca
     5221 gctcaaattg atggtggtta tagtggcagg gattgccgct aagaattggg ctaattcagc
     5281 gcctgttttt ttgtcaagca tattgttgca caaatgaagg gaggtcacgc tggcggagat
     5341 tgccgcaaag gcttgggcta attcagcacc tgtctttttg tcaaggtgat tccagctcaa
     5401 ctgaagggaa gtcacactag cggggattac tgcaaaagct tgagctaatt cagtgcctgt
     5461 tttattgcca agcttactgt ctctcaaatc aaggtaggtg acattgccag ggatattctt
     5521 gatagcttcg cttaattgcg aaccattaag ttcactaagg tttagctggt aatacatcta
     5581 aatctcctgt gcttttcatc gatattgggt ttggacgcca tttatatcta aaattaaat
//
LOCUS       DBNCWK010000161         5582 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.162, whole genome shotgun sequence.
ACCESSION   DBNCWK010000161 DBNCWK010000000
VERSION     DBNCWK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.162"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            150..584
                     /locus_tag="AC06IO_002611"
     CDS             150..584
                     /locus_tag="AC06IO_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIO9875778.1"
                     /translation="MKLWKVLCSFVIAVCYLPLAFAASQSPAGTWTTIDDKTGAKRAV
                     VNLKVSNGTLSGTIVKVYPQPGDTGICSKCPGAFKDKKIQGLTFVWGLKDEGNGVWSG
                     GSILDPKTGKIYKAKMTLQGNKLYVRGYFGVSALGRTQTWIR"
     gene            762..2144
                     /gene="dnaB"
                     /locus_tag="AC06IO_002612"
     CDS             762..2144
                     /gene="dnaB"
                     /locus_tag="AC06IO_002612"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIO9875779.1"
                     /translation="MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNK
                     LCETDFYRTEHRILFRAISALAKKDQPFDVVTLLDALKTRNELDDAGGEAYLFELANN
                     TPSVANVTAYADIVREKSVQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGE
                     QTASDGGPENIKSILVRAVEKIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVA
                     GRPSMGKTTLVMNMAEHAAIKAGKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGK
                     LDDNDWPRVTSAVHMLSEAPLFIDDTPALSPGEMRARARRLAKEHGSLGLIVVDYLQL
                     MKVPGFNADNRTAEISEISRSLKSLAKELQVPVIALSQLNRSLEQRADKRPVMSDLRE
                     SGAIEQDADLICFIYRDEVYNEDSPDKGTAEIIVAKQRNGPIGKVRVAFLGKYTRFED
                     LAFNGYQGVE"
     gene            2146..3219
                     /gene="alr"
                     /locus_tag="AC06IO_002613"
     CDS             2146..3219
                     /gene="alr"
                     /locus_tag="AC06IO_002613"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIO9875780.1"
                     /translation="MSRPTRLVIEPSALLHNLSQIKHLAPGKKVIAMVKANAYGCGVR
                     EVAPVLDGRIEAFGVACLEEALAIRALGVETSCILFQGVFSSDELSVAVENDFACILH
                     HAQQLEWLIKTPLPYPIKIWVKVNTGMHRLGFKIHELQKVMGALQTCTWVDKNIGLMT
                     HLACADEPHRPENQQQISLFQEISIPGFRQRSIANSAAIISFPDSHADVVRPGIMLYG
                     VSPFANQNARDLSLIPVMRFMSAISAIHDNPSFAQVGYGGTWKSDKPSRIGIVAAGYG
                     DGYPRHISEKTPVWVRGREVSIVGRVSMDMLTIDLTEHPDVEIGDEVELWGTHVLVER
                     IAKSAGTVGYELLCQISERVRYK"
     gene            3292..3741
                     /locus_tag="AC06IO_002614"
     CDS             3292..3741
                     /locus_tag="AC06IO_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HIO9875781.1"
                     /translation="MKKLIVASLSLTFMLGGCASMNKEGVGTITGGVVGGLLGSQFGG
                     GSGKVAAAGAGALLGAYLGGQIGKTMDKVDRLEMQRALETAPTGKAVAWSNPDTGYRY
                     TVQPTRTYYNAQQPCREYITKAMIGGKTQQIYGKACRQADGSWRVVN"
     gene            4047..4481
                     /locus_tag="AC06IO_002615"
     CDS             4047..4481
                     /locus_tag="AC06IO_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIO9875782.1"
                     /translation="MAHLIHSVLPVPPRKIAYIHPDDSVKECIKQMVEKDIGALVVFD
                     NDARLIGIVSERDILRCYFHKNLSLETAKVSDVVYTNVTILSPHDSVEKAMQVITETK
                     RRHVLIQEEGELLAILSIGDLLYHVLEDKLRVIEHLENYIHS"
     gene            4597..>5582
                     /locus_tag="AC06IO_002616"
     CDS             4597..>5582
                     /locus_tag="AC06IO_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875783.1"
                     /translation="MKTMKQMIRLTMLLLMLSFNLWAINSSSPWYTEEKGKGLVINVE
                     LYLSSTCPHCHKADEFFKEIEKHYTWLHVERYIIDKDKKALKRFGDLLTELNRYDFAV
                     PSIFFCNSRWVGFATAQTTGKDLLNGIEYCKEEIVKNGRLTPATESVLNRWGNANLFD
                     SNITGSPSTNTFIFVVALIDAISPCALFTIAAFLGVLFMIEKRKLQFISGSVFILTVA
                     GVHYFQQVYPTLFFESLSWFRIPAALVGLFAFYFAGQYYKKHSIQPLFIVLAFLLALT
                     VPMFQQTCLMNWSYVFEQWLHNQNVSGVQMGLYQFAYQLIYIFPLIILMFLY"
BASE COUNT         1635 a          966 c         1230 g         1751 t
ORIGIN      
        1 accctatatt tattttaaag atttcagtag caaagttacc tatcatggct tatgctttat
       61 tacaagctct tgtataactt gaactttgta ttccaaaaga gtacagaatt tatacaagag
      121 gtatactgat tcaattacgg agattactta tgaaattatg gaaagtgtta tgtagttttg
      181 taattgctgt atgttattta ccattagcct ttgcagcatc acagtctcct gccggtacct
      241 ggacaaccat tgatgataaa accggagcta agagagcagt agttaacctt aaagtatcaa
      301 atggtacttt aagtggtact attgtgaagg tatatccaca accaggtgat accggaattt
      361 gttctaagtg ccctggcgca ttcaaggaca aaaaaattca agggttgact tttgtttggg
      421 gattaaaaga tgaaggcaac ggtgtctgga gtggtggctc tatcctcgat cccaaaacag
      481 gaaaaattta taaggctaag atgacactgc agggtaataa actgtatgtc agagggtatt
      541 ttggagtttc tgctctagga cgtactcaaa cctggattcg ataagtagta ctgaaggatg
      601 tttttatata taatgtggga tagtgctttc tcttgtcatt gaaagcagtt ggtactatcc
      661 cgcattttgt ttttagtgtt atgatcattt gcaattagat taggaatgtc tttaaataaa
      721 ttgtcttccc tataaataaa ttctcaaaaa atattattac catgcttgat ttgcaaatat
      781 ctaaaaaaac ggttgatcca ctaaaacgcc cccctcattc tgtagaggct gagcaatcca
      841 ttattggtgg tttgatgctc gataatcaag tctgggataa ggtaagcaat aaattatgcg
      901 aaaccgattt ttatcgtact gagcaccgaa ttctttttag agccatttct gctttagcca
      961 aaaaagatca accttttgat gtggtgacgc ttcttgatgc tttaaaaaca cgcaacgagt
     1021 tagatgatgc tggtggagaa gcctatttat ttgagttggc taataacaca ccaagtgtag
     1081 cgaatgtgac cgcttatgcg gatattgttc gggaaaaatc agtccagagg caactgatag
     1141 cggtagcaac agaaattgct gattccgctt ataatcctgc tggaagagaa gtgcctgagc
     1201 tattggatat ggctgaaacc aaagtatttg ctattggtga acaaacagcg agtgacgggg
     1261 gtccagaaaa tattaaatcc attttggtac gggcggtaga gaaaatagat gccctctatc
     1321 ataatggtga tgcgattaca ggccttgcga caggcttatc cgatttggat gaaatgacat
     1381 cgggtttaca gccttctgat ctgattatcg ttgctggccg tccatcgatg ggaaaaacaa
     1441 ctttggtgat gaatatggca gagcatgcgg caattaaagc cggtaagcca gtattggttt
     1501 tctctatgga aatgcctgct gattccctgg caatgagaat gatgtcttcc ttggggcgaa
     1561 tagatcagca tcggattcgt actggtaaat tagatgataa tgactggcca cgagtgactt
     1621 ccgcggtgca tatgctttct gaagctcctt tgtttataga tgatactcca gctttaagtc
     1681 ctggagaaat gagggcccgt gccagacgtt tagcaaagga acatggttcg ttagggctta
     1741 tcgttgtcga ttatttacaa ttaatgaaag tgcccgggtt taatgcagac aatagaacag
     1801 cggaaatctc cgaaatttct cggagtctta aatcgctggc aaaagagtta caggttcccg
     1861 taattgcttt atctcagctg aatagaagcc tcgagcagcg agcagacaag cggccagtga
     1921 tgtcagattt gcgagaatca ggagcaattg aacaggatgc tgacttaatc tgttttattt
     1981 atagagatga agtatataat gaggacagtc ctgataaagg cactgcggaa atcattgttg
     2041 ccaagcaaag aaatggaccc ataggcaaag tcagggtggc ctttttgggt aagtacacac
     2101 gatttgaaga tttggcattt aatggttatc aaggggtaga atagggtgtc aagacctaca
     2161 cgtttagtga tagagcccag tgctttattg cataatttat ctcaaatcaa gcatttagct
     2221 ccaggcaaaa aagttatcgc tatggtaaaa gccaatgctt atggttgtgg agtcagggaa
     2281 gttgcccctg tccttgatgg gcgtattgag gcttttggag tagcctgcct tgaagaggct
     2341 ctggctattc gagctttagg tgttgaaacc tcgtgcattc tctttcaggg cgtctttagt
     2401 tctgatgaat tgtcagtagc cgttgagaat gatttcgcct gtattcttca tcacgcacag
     2461 cagttggaat ggctaatcaa gactccgttg ccttacccga taaaaatatg ggtgaaagta
     2521 aacacaggca tgcacagact gggatttaaa atccatgaat tgcaaaaagt gatgggggca
     2581 ttgcaaacct gtacctgggt tgataaaaat atagggttaa tgactcattt ggcttgcgca
     2641 gatgagcctc atcgtcctga gaatcaacaa cagatctctt tgtttcagga gatatccata
     2701 cctggatttc gccaacgcag tatcgctaat tcggcggcaa ttatttcatt ccctgattcc
     2761 catgctgatg tggtgaggcc cgggattatg ttgtatggtg tttccccatt tgccaatcaa
     2821 aatgcccgtg atttaagttt aatccctgtc atgcgtttta tgtcagccat cagcgcgatt
     2881 catgataacc catcttttgc tcaggtaggc tatggtggaa cctggaaaag cgataaacct
     2941 tcgcgaattg gcattgttgc agcaggttat ggcgacgggt acccacggca tatttccgag
     3001 aaaacaccgg tttgggttag agggagggaa gtgtctattg ttggcagagt ttctatggat
     3061 atgctgacca tcgatttgac agagcaccct gatgttgaga tcggcgatga agttgagctt
     3121 tggggaacac atgttttagt agagcgcatt gccaaatcag caggtacagt aggatacgaa
     3181 ttattatgtc aaatttcgga gagagtacgt tacaaataag ttatggataa taactgttcg
     3241 tgatggtaga atgacaaccg attttgtaac tctttaaaca ggtgaactgt aatgaaaaaa
     3301 ttaattgttg catcattatc cctaacattc atgctaggtg gctgtgcctc aatgaacaaa
     3361 gaaggtgtag gtacaattac aggtggtgtg gtaggtggat tacttggtag ccagtttggt
     3421 ggtggttcag gtaaggttgc agctgcagga gcaggagcat tattaggagc ttacctgggt
     3481 ggtcaaattg gaaagacaat ggataaagta gaccgacttg aaatgcaaag ggccctcgaa
     3541 acagctccaa caggtaaagc agttgcatgg tcaaatccgg atactggata ccgttatact
     3601 gtccagccaa cgagaactta ttataatgct caacaacctt gccgtgagta tattaccaaa
     3661 gccatgattg gtggtaaaac ccaacaaatt tatggtaagg cctgtcgcca agctgatggt
     3721 tcctggcgtg ttgtgaatta attaattgat taagattgtt ggggaaaacc caacaatctc
     3781 attttattac tcaaattaat taagacagga cttacgaaaa actcctatct ctatgttaaa
     3841 aatattttta attcagtcat ttagtttatc taaattcctt cattaaaatc attttttgcc
     3901 ttgaccttgg agttttcaat atacaaatcc tacgataatc ttttataaaa atctttcctg
     3961 ccctcacaca atgagcttaa ccttccttgt tttgtgctat atttatacag cgagttgcta
     4021 atttagtatt aatcagggag agtgcaatgg ctcacttaat tcatagcgta ctgccggtgc
     4081 caccaaggaa aatcgcttat attcatcctg atgatagtgt caaagaatgt attaaacaga
     4141 tggttgaaaa agatataggt gctttagttg ttttcgacaa tgatgcccgc ttaattggta
     4201 ttgtgagtga aagagacata cttcgctgtt attttcataa gaatctaagt ttggaaactg
     4261 caaaagtatc tgatgttgtt tataccaacg tcacgatctt aagtccacat gattcggtag
     4321 agaaagccat gcaagtgata actgaaacaa aaagaagaca tgtattaata caggaagaag
     4381 gtgagttatt agctatactg tcaataggag acttgttgta ccacgtactg gaagataaat
     4441 tgcgagtgat tgagcatttg gaaaattata ttcactcctg aaatataagc ttttatttca
     4501 gatttacatt ttattgcttt aaacgcgaga gtcttgtttt ggctatttca tacaatcgat
     4561 cctaagtgct atagtattca tttacaaaga aatataatga aaaccatgaa gcaaatgatt
     4621 cgattgacaa tgttacttct gatgctcagt tttaatttat gggcgataaa ttcttcatct
     4681 ccctggtata cagaagaaaa aggtaaaggg cttgtaatta acgttgaatt gtatctttca
     4741 tctacttgtc ctcattgtca taaggccgat gaatttttca aagaaattga aaaacattat
     4801 acctggctgc atgttgagcg ttatattatt gataaagaca aaaaggcttt gaaacgcttt
     4861 ggcgatttat tgacagaact taatcgatat gattttgccg ttccatccat atttttttgt
     4921 aattcccgtt gggttgggtt tgccactgca cagacaaccg gaaaggatct gttaaacggc
     4981 atagaatatt gtaaagaaga aattgtgaaa aatggaagat tgactcctgc gaccgagagt
     5041 gttttaaaca gatgggggaa tgcgaatttg tttgattcca atatcacagg aagcccttcc
     5101 acaaatacat ttatttttgt tgtagcctta atcgatgcaa tcagtccatg tgctttattt
     5161 acgattgctg catttttagg tgttttattc atgattgaaa agcgaaagct gcaatttatt
     5221 agcggttccg tgtttatttt aactgttgca ggtgttcatt attttcaaca ggtttatcct
     5281 accctgtttt ttgagtcgct ttcatggttt cgtattccgg ctgcattggt aggccttttt
     5341 gctttttatt tcgctggcca atattataaa aaacattcca tccaaccatt atttattgtt
     5401 ttggcttttt tattggcact tacagtgcca atgtttcaac aaacctgttt gatgaattgg
     5461 tcttatgtgt ttgaacaatg gttacataac caaaatgtat caggagttca gatggggcta
     5521 tatcaatttg cttatcagct tatctatatc tttcctttaa ttatcctgat gtttctctat
     5581 tg
//
LOCUS       DBNCWK010000162         5520 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.163, whole genome shotgun sequence.
ACCESSION   DBNCWK010000162 DBNCWK010000000
VERSION     DBNCWK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.163"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..934
                     /locus_tag="AC06IO_002617"
     CDS             <1..934
                     /locus_tag="AC06IO_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg1803 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875784.1"
                     /translation="SQYTRLFEFLCDLIDIQIKKYVELAKLKVGEDAIINAALIYEEV
                     NELLGFAEKLLKEIETNSKSDSMDMTIFDRVFEQVKFYVEQEYLRSYAEWLLIENNSK
                     DTVYNRTKEQLLRLSEIKSFDDKGFSQPSTEIQQQCQHDSVQIVYSILNLAREVRKNP
                     DMGFDDSIPHNKLNVLRRNATTRYVEFGNEIDKLHQHSEEFLQQSSLGVFDAELAYES
                     ALSKAKDHDLKLNTLFGRYKENKETASVPQSLSLVNKVDEENIMTEKKPSTEVSDNKT
                     PFFSTSNLLFFGGIVLAAVIFVPRLLTHLKVNKL"
     gene            1237..2283
                     /gene="recA"
                     /locus_tag="AC06IO_002618"
     CDS             1237..2283
                     /gene="recA"
                     /locus_tag="AC06IO_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIO9875785.1"
                     /translation="MEENKQKALSAALSQIERQFGKGSVMRMGDSTVSRDIEAISTGS
                     LGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLQVIAECQKMGGTAAFIDAEHALDPS
                     YAQKLGVKVDELLVSQPDTGEQALEITDMLVRSAAVDVVIIDSVAALTPKAEIEGEMG
                     DSHVGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRIGSIKKGEEILGSETRVKVVKNKVAPPFKMTEFDILYNEGISRESEIIN
                     LGVQLNLIEKSGAWYSYKQEKIGQGKENVRLYLKENPQVAAELEQQIRTELLEKKLSV
                     LASSSEDLFETIDD"
     gene            2276..2728
                     /gene="recX"
                     /locus_tag="AC06IO_002619"
     CDS             2276..2728
                     /gene="recX"
                     /locus_tag="AC06IO_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIO9875786.1"
                     /translation="MTKAFDSALRLLTRREYSAMELCDKLKQKGFSTNDVQNALYECQ
                     RLGYQSDVRFVENYIRVRIHQGYGPLKIRQELKNKGIDPDLIQSVLHQEKDNWVNYAL
                     RAWEKKFKRQDDFSYSEIQKQQRFLLYRGFDRDVISKVFKEVKSSYLI"
     gene            2746..5328
                     /gene="alaS"
                     /locus_tag="AC06IO_002620"
     CDS             2746..5328
                     /gene="alaS"
                     /locus_tag="AC06IO_002620"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIO9875787.1"
                     /translation="MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQ
                     FKDLFLGLETRSYQRAATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFW
                     SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHP
                     LPKPSVDTGMGLERLAAVIQGVHSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADH
                     IRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFFSKLVQPLIDVMGDA
                     YPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYG
                     FPIDLTADIIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGY
                     EKESMEAKIIGLLQEGNEVKSINKGAKGAVILDHTPFYAESGGQVGDKGLLIGKEFTF
                     QVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQVDNIRRDAIRLNHTATHLLHAALKKI
                     VGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQIEEVVNAQIRANNEVITQVMDIES
                     AKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIR
                     RIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEKVSQLLLDNKNQEKMIAKLLS
                     EKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQ
                     NKMNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGGGVPEDLNSKIKEIKEM
                     IEKI"
     gene            5419..>5520
                     /locus_tag="AC06IO_002621"
     CDS             5419..>5520
                     /locus_tag="AC06IO_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HIO9875788.1"
                     /translation="MNDLLNLIAVIVVFGVGLWLINVFIPMPGAIKSL"
BASE COUNT         1786 a          871 c         1214 g         1649 t
ORIGIN      
        1 gagtcaatat acaaggttgt ttgagttttt atgtgatctg attgatatcc agattaaaaa
       61 atatgttgag ctcgcaaaac ttaaagtagg tgaagatgcg ataataaacg ccgctctcat
      121 atacgaagag gtgaatgaat tattaggatt cgcggagaag ttattaaagg aaatagaaac
      181 caattcaaaa tccgactcaa tggatatgac catttttgat cgtgtttttg agcaagtaaa
      241 attttatgtg gagcaagaat atctgagatc ctatgcagaa tggctgctta ttgaaaataa
      301 ctctaaagac acggtctaca atagaacaaa agagcaatta ctcaggcttt cagaaattaa
      361 gagttttgat gataaaggtt tttcacaacc aagcactgaa attcaacagc aatgtcaaca
      421 tgatagtgtt cagatagttt attccatttt aaatttggca agagaggtaa gaaaaaatcc
      481 ggatatgggg tttgatgata gcatcccaca taacaagctc aatgtattaa gaagaaatgc
      541 aactaccaga tatgttgaat tcggaaatga aatagacaaa ttgcatcagc atagtgaaga
      601 atttttgcaa cagtcgagtc ttggagtttt cgacgccgaa ttagcctatg aatcggcact
      661 atcaaaggca aaagaccatg atcttaaatt aaatactttg tttggtcgtt ataaagaaaa
      721 taaggaaacc gcttctgttc ctcaatctct ctctttggtc aataaggtag atgaggaaaa
      781 tattatgaca gaaaaaaaac cttcaactga agtgtctgat aataagaccc ctttcttttc
      841 aacaagcaat ttattatttt tcggtggtat agttttagcc gctgttattt ttgttcccag
      901 attgcttact catctcaagg tcaataaatt atgacacagc attatgagta gttaacattt
      961 tcgttatttt gattgtaaaa ccttggtttt tatgtcggat tgtctatata tttttatatt
     1021 gccccgttac caagctaaaa atcaaggttt gaagttcaaa ctgacaacac cttcattttt
     1081 gaacaaatgg ttattccacg tagcttataa taaaagacta atcatcagct atcgtatgct
     1141 acttttattt agtaatacac taagattact gtttgctgct cgctcacatc tgtgtgataa
     1201 ttttgttctt gcacacatta taatctggaa taaattatgg aagaaaataa acaaaaagca
     1261 ctatcagcgg cgctttcgca aattgaacgc caatttggca aagggtctgt aatgcgtatg
     1321 ggagatagca cggtatcacg ggatattgaa gcaatctcta caggttcatt aggtcttgat
     1381 attgccttag ggataggcgg tttgccaaaa ggccgtattg ttgaaattta tggccctgag
     1441 tcttctggta aaaccacgct cactcttcaa gtgatcgctg aatgtcaaaa aatgggtggt
     1501 acagcagcat ttatcgatgc ggaacatgct ctggatccaa gctatgctca gaaacttggt
     1561 gtgaaggtgg atgagctgtt ggtttctcag ccggatacag gcgagcaagc tctggaaatt
     1621 actgatatgc tggtgcgttc tgcagcagtt gatgtcgtaa taattgactc ggttgctgcc
     1681 ttgactccca aagcagaaat tgagggagaa atgggcgatt cccatgttgg tttacaggca
     1741 agattgatgt cgcaagccct gcgtaaattg accgctaaca ttaaacgttc gaacacactg
     1801 gttattttta ttaatcaaat acgtatgaaa attggtgtga tgtttggtag ccctgaaact
     1861 actacagggg gcaatgcttt gaagttttat gcttcagttc gtttggatat tcgccgtatt
     1921 ggttcaatta aaaagggtga agaaatatta ggcagtgaaa cccgagttaa agtagttaaa
     1981 aataaggtag cgccaccgtt taaaatgact gagtttgata ttttatataa cgaaggtata
     2041 tctcgtgaga gtgaaattat taatctgggt gttcaattga atttaattga gaaatcaggt
     2101 gcctggtata gctacaaaca ggaaaaaatc gggcagggca aagaaaatgt cagattatat
     2161 ttgaaagaaa atccccaagt cgctgcagag cttgaacaac aaatccgtac tgagctcttg
     2221 gaaaaaaagt tatctgtgtt ggctagttca tcagaagatc tttttgagac tatagatgac
     2281 taaagcattc gatagcgcct tgcgtctgtt aaccaggcgc gagtatagcg ctatggagtt
     2341 atgtgataaa ctgaagcaga aaggatttag caccaatgat gttcaaaatg ctttgtatga
     2401 atgccaacgt cttgggtatc aaagcgatgt gcgttttgta gaaaattata tcagagtacg
     2461 aattcatcaa ggctatggcc ctttaaagat caggcaggaa ttaaaaaaca agggaataga
     2521 tccagatctc attcaaagtg tgctgcatca agaaaaagac aattgggtca attatgcttt
     2581 gcgggcatgg gagaaaaaat ttaagagaca ggatgatttc tcttatagtg aaatacaaaa
     2641 acagcaacgg tttttgctgt atcgagggtt tgatagggat gttatttcaa aggtatttaa
     2701 agaggttaaa tcttcttact taatttaact ggtaaatatt aaaaaatgaa aagttctgaa
     2761 attagacaag cattccttaa ctattttgtt caacgcggtc atcaaatagt agcttctagc
     2821 tcattggtcc cttcaaatga tcccacttta ctatttacca atgcaggtat ggtgcaattc
     2881 aaggatcttt ttttaggtct tgaaactcgt tcatatcagc gtgctgctac agcacagcgc
     2941 tgtgtgcgtg caggtggtaa acataatgat ctggaaaatg ttggttatac ggcgagacat
     3001 catacatttt ttgaaatgtt gggtaatttt agttttggtg attattttaa acgcgaagct
     3061 attcaatatg cttgggaatt cctaacagaa gttttacaca taccggcaga acggctatgg
     3121 gttactgtct ataaagagga tttggaagca gaagacatat ggctaaagga aatgaaggta
     3181 tctcccgagc gattctctcg atgtggggaa aaagataatt tctggtcaat gggggatacg
     3241 ggtccatgtg gtccttgcac agaaattttt tatgatcatg gtcctgaagt agcaggtggt
     3301 cccccgggca gtccagatga agatggagat cgttatatag aaatatggaa cctggtattc
     3361 atgcagttta acagggacag agagggacat ttgcacccct tgccaaagcc ttctgtggat
     3421 actggtatgg gattggaacg attagccgca gttattcaag gtgtccatag taattatgaa
     3481 attgatagtt ttcagtattt gattaaagca atagcacaat tgggccagga tattgattta
     3541 aatcatactt ccttaaaagt gattgcagat catattcgtt cctgctcttt tttgatagtt
     3601 gatggggtat tgcctagtaa tgaaggcaga ggatatgtat taagaaggat tatccgcaga
     3661 gcagtcagac atgggaataa attaggctta cccagcccat ttttttccaa acttgttcag
     3721 cctttgattg atgttatggg ggatgcttat cccgagctaa tcaattcgaa agcgcatatt
     3781 gaaaggatct tgcaacaaga agaaaatcag tttacccgta ctttggaaca aggattgcgt
     3841 ttattacaag atcatattaa aaatttgcaa ggccaggaac tatcaggaga agtggcattc
     3901 aagttgtatg atacttatgg tttccctatc gatttgacag cagacatcat tcgtgaacaa
     3961 ggtttacata ttgatatgga agcatttaat caattgatgc aacaacaaag agaacagtct
     4021 caagccgcca gtcagtttac tacagattat cacgccgtgt ctcaattgga tcatcagtct
     4081 gaatttcatg ggtatgaaaa agaatccatg gaagcaaaga ttattggatt acttcaagaa
     4141 ggtaatgaag tgaaatctat taataaggga gcaaaagggg ctgtaattct tgatcatacc
     4201 ccattttatg ctgaaagtgg tggtcaggta ggagataaag gattactcat aggaaaagaa
     4261 tttacttttc aagtcgatga tacacaaaaa gtagggcaag ctgtcgtaca ttatggaaaa
     4321 gtaattaaag gtgagctcac tttagattta ttaatccatg cgcaagtaga taacataaga
     4381 cgggatgcaa ttcgattgaa tcataccgca actcatttgt tacatgctgc tcttaaaaaa
     4441 attgtagggc aacatgttca acaacgagga tccctggtgg atgctgaaag ggcacgtttt
     4501 gacttttctc atttcgaggc attgactcct caacaaattc agcaaattga agaggtagtt
     4561 aatgcacaga tccgagctaa taatgaagta ataactcaag taatggacat tgaatcagcc
     4621 aagcaaagtg gtgctgttgc tttattcggg gaaaaatact ccgacgcagt acgcgtatta
     4681 tccatgggtg atttttccaa ggagctttgt ggaggaactc atgcacgacg tactggtgat
     4741 ataggtcttt tcaaaatcgt agcagaatat ggtattgcca gcggtatcag gcggattgag
     4801 atggttacgg gtcgttatgc attagcctgg gtcaatgaac agctgggatt tatgaataat
     4861 ctggcagcta cccttaaaac aacacctaat agtctacagg aaaaagtttc tcaattattg
     4921 cttgataata aaaatcaaga aaaaatgatt gcaaaacttt taagtgaaaa agcacaaaaa
     4981 tctggagcag atattcttgg tgagatcgag gagatcaagg gtattaattt attgattaag
     5041 cagttagagg gtatggatag ccaaaccatg cgccatacca tggatcaatt aaaatcaaga
     5101 attgattcag cggtgatcat attatttact attgaacaga ataaaatgaa tgtcattgcc
     5161 ggggtgagta agaatattat tggcaaagca ccaagtgctg ctcaattagt aagacatctt
     5221 tgtggtaaag gaggtggtcg agatgatatg gctcaaggtg gtgggggtgt tcctgaagat
     5281 ttgaacagta agatcaaaga aataaaggaa atgattgaaa aaatttaata tttccagatt
     5341 tttcttttcg actgcagaaa agcaagttgt ttttggattt gctataatat agacactggt
     5401 ttactaatga gcactatgat gaatgacttg ctgaatttaa tcgcagtgat agtggttttt
     5461 ggtgtaggcc tatggttaat taatgttttt atacctatgc ccggggctat taaaagtctg
//
LOCUS       DBNCWK010000163         5461 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.164, whole genome shotgun sequence.
ACCESSION   DBNCWK010000163 DBNCWK010000000
VERSION     DBNCWK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.164"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(228..1121)
                     /locus_tag="AC06IO_002622"
     CDS             complement(228..1121)
                     /locus_tag="AC06IO_002622"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIO9875789.1"
                     /translation="MKHFLEISQLSSEQIESLLQRALYFKHTKQYPSYFQSIIANLFY
                     ENSTRTRISFELAERHLAMSVVNLDLQTSSETKGEAIEDTIRTLAAMGIQYFVIRHKQ
                     DGLQQNLANKLGDTVHIINAGDGTHAHPSQAILDMVTIIEQKKRLDKLKIAILGNIKH
                     SRVANSFQCICSKLGVGELVLISPEIWQPSQVHFGRVTDNLNEGLEGADVIICLRVQR
                     ERLLQDDHLDLDFYRNNFALTQKSLSYAKPDAMVMHPGPMNRGVEIDSEVADGKQSCI
                     LQQVTNGVYARMAILESLIGS"
     gene            complement(1171..1590)
                     /gene="ruvX"
                     /locus_tag="AC06IO_002623"
     CDS             complement(1171..1590)
                     /gene="ruvX"
                     /locus_tag="AC06IO_002623"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIO9875790.1"
                     /translation="MPRGVYLGFDFGYKRIGVAVGQRLTCSASPLSTIEAKAGIPDWN
                     SIQKVITQWNPQALIVGLPTCIDDRELYTTSAARRFAKQLHKRFSLPVHLVDERLSTV
                     EARGYLFEQGGYRQIKKAEVDSIAACVILEQWLQQSE"
     gene            complement(1583..2146)
                     /locus_tag="AC06IO_002624"
     CDS             complement(1583..2146)
                     /locus_tag="AC06IO_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIO9875791.1"
                     /translation="MAIISSLANHLLIAMPSLKDPNFERSVVYLCEHNEQGSVGLIIN
                     RPLQFPLSIVFEQLQIEPIRVEKNGLPLLFGGPVQPERGFVIHKQMGGWRSSLFLQDE
                     VTVTTSNDIIRAIAYDEGPKDVLITLGYAAWTEQQLEREIMSNTWLVCPYKSEILYEV
                     PFEERWEYAGLTLGIKMNQLSSDAGHA"
     gene            complement(2450..3214)
                     /locus_tag="AC06IO_002625"
     CDS             complement(2450..3214)
                     /locus_tag="AC06IO_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/III secretion system protein"
                     /protein_id="HIO9875792.1"
                     /translation="MIKRWLLVILLISGSAFSQQMITKVIELNYLQADKVIQLIQPLL
                     QTDEKVSGSGQTLIVNVSPQTLTQIRNVLHKIDVPPVVFNITVYQGDPEWLSSQDGND
                     VVYSTQPQYEVQRSQSVKVMSGESAFVATGEQVPVVTSVGVGLLATGVSYEQHNIQNG
                     LLVHPVLQGSQVKLTVRRVREQENVESDQQFDNQQIDTTIMAPLNKWVPLGTAEGVQN
                     TDSSSTSYTAGRPFSQNSTLYIKVSVVKSTPSGTTK"
     gene            3402..4073
                     /locus_tag="AC06IO_002626"
     CDS             3402..4073
                     /locus_tag="AC06IO_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HIO9875793.1"
                     /translation="MGSIFNMFGPSPIKPIEQHIRKAHQCAKQLYPFFEAVLKNDWDT
                     ANKIKDKIISIEKEADLIKRDLRLHLPTGLFLPVARTDILELLSAQDRIANKAEDIAA
                     LIISRQMSIPKKLIPSFMPFLNRCLDASKQACTAINELDELLETGFRGSEVKIVEEMI
                     VKLDEIEHDCDERLADIRHKIFELEKELPAIDVIFLYKLVQWIGELADHAQTVGGRLQ
                     ILIAR"
     gene            4083..5336
                     /locus_tag="AC06IO_002627"
     CDS             4083..5336
                     /locus_tag="AC06IO_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIO9875794.1"
                     /translation="MDYSIYLLFAALILCFLMTWGVGANDLANVMSTTMGSKAVTVRQ
                     AMLIAIIFEFAGAFLGGEGVTETMRDGIINTSQLSNEPLILIEGMLCVLFACTIWMNL
                     ASYLGVPVSITNALVGSMVGFGTIVLGPDAIHWNQVARIAIGWVSSPLISGITAYALF
                     ISIQQTIFVKSNPLTKAKLYIPIYLFLIGFILSFITVFKGLNHFDIHLNLKQDLAVTL
                     ATSIIITILGMIFIKRIPEYHKIRRRERFIQVEKYFAVLMAMTACAMAFAHGSNDVAL
                     AVGPLSIVHSLVMHSNQIFDADNYPSWIILLGCVGVVTGFLMYGRKVIETVGSSITAL
                     TPSRAFAATLSAATTVVVATSTGIPVSATQTLVGAVLGVGLARGIGALNLIVIRNIFM
                     SWVLTLPAASMLTILSYKLLHALLG"
BASE COUNT         1646 a         1136 c         1002 g         1677 t
ORIGIN      
        1 aacaaaaaaa tctgatcatg ggaaaataac agtttttatg ataaataata aaggaattat
       61 gctttattgc atcggcactg caggggaaaa actaaaaata aatgcctgtg cttctgatgt
      121 aggcaagcca cttactcccg aacaagaatt gtcaattgag ggcttctttt aactagccac
      181 taatcagcaa gaaacgaaag aaaaatcatg gccggatata aagaaattca acttcctatc
      241 agcgattcaa ggatagccat tcttgcataa acaccattag taacctgttg taaaatacaa
      301 gattgcttgc catcagccac ttcactatca atttcaaccc cgcgattcat aggcccaggg
      361 tgcataacca tagcgtcagg ttttgcataa gacagacttt tttgagtaag tgcaaaattg
      421 tttctataaa aatccaaatc caaatgatca tcttgcagca aacgttccct ttgaactcgc
      481 agacaaatta tcacatccgc accttccaga ccttcattga gattatcagt aacccgtcca
      541 aagtgcactt gtgatggttg ccaaatctct ggagaaatca aaaccaactc accaacccct
      601 aatttactgc aaatgcattg gaaagaatta gcaacacgtg aatgtttaat atttcccaaa
      661 atagcaattt ttaatttatc taatcgcttt ttttgttcaa taattgtcac catatccagt
      721 atcgcctggc ttggatgagc atgagttcca tcacctgcat ttattatatg aactgtatca
      781 cctaacttat tagctaaatt ttgctgcagc ccatcttgct tatggcgaat tacaaaatac
      841 tggataccca tagcggctag cgttctgatg gtatcttcaa tggcttcgcc tttagtttct
      901 gatgaggtct gaagatccag attcactaca gacattgcca gatggcgctc tgctaactca
      961 aaactgatgc gggttcgggt actgttttca taaaataaat tagcaataat actttggaaa
     1021 taagaagggt attgctttgt atgtttaaaa tataaagctc gttgcaataa ggattcaatt
     1081 tgttcagatg acaattgact aatttcaaga aaatgcttca tgttcccccg ccaaatttca
     1141 ttttacgcca aaagaaaaat actctttgag ttattccgat tgttgcaacc actgctccag
     1201 tataacacaa gctgctatac tatctacctc ggctttttta atctggcgat atcccccctg
     1261 ctcaaacaaa taccctcttg cctctactgt tgaaagtcgt tcatcgacta aatgcaccgg
     1321 caaagaaaac cgcttgtgca attgctttgc aaaacgacga gcagctgatg ttgtatacaa
     1381 ttcacggtca tcaatacagg taggaagacc tacaattaaa gcttgtggat tccattgggt
     1441 tatcactttt tgaatggaat tccaatcagg gattccggct ttggcttcta tagtagataa
     1501 gggagaggca ctacaagtta gccgctgtcc tacagcgact cctatgcgtt tatacccgaa
     1561 atcaaaacct aaatacacac ctctaggcat ggccagcatc agaagagagt tgattcattt
     1621 tgatacccag cgttaatccc gcgtattccc aacgctcttc aaagggcact tcataaagaa
     1681 tttcagattt atagggacaa actaaccacg tgttactcat aatttctctt tccaattgct
     1741 gctctgtcca agcagcataa cctaaagtga ttaatacatc tttagggcct tcgtcataag
     1801 ctatggcacg aatgatatca tttgaggtag taaccgtcac ttcatcttgc aaaaacaaac
     1861 tggaacgcca cccgcccatt tgtttatgaa taacaaatcc tctttctggt tgtactggtc
     1921 cgccaaacaa taaaggcaac ccattttttt ctactcgaat aggttcaatt tgcaattgct
     1981 caaaaacaat agatagtgga aactgtaaag gtctgttaat aattaaacca actgacccct
     2041 gctcattatg ctcgcacaaa tagaccactg acctttcaaa gtttggatct tttaaggaag
     2101 gcatggcaat caataggtga ttggccagtg aactgataat tgccataaga agtccccccc
     2161 aaaattctct ccgctcactt taattataca ccattaatga ttttaatttt atcattttgt
     2221 ttttaattta atctatgcgt cgtggcatga gcccacaata ccaaattatt atgattttca
     2281 tggcttaaag atgcttacta gaaactccag gatcaaggca aaaatatttt taatgaaggg
     2341 attaggagtt agttaatcag taaatcctgg cttattctcc agttcaggga agaaaagctc
     2401 attaagagaa gatcgaagta aaatgatttt gaaacgcatt tccatcattt tatttggtgg
     2461 tcccagaagg ggtggatttt acaactgaga ccttaatgta caaagttgaa ttttgagaaa
     2521 aaggccttcc agctgtataa gaagttgaag aagaatcggt attttgaacc ccctctgctg
     2581 tacctaatgg aacccattta ttcaatggcg ccatgatcgt agtatctatt tgttgattat
     2641 caaattgttg atcactctct acattttcct gctctcgcac tcttctcacc gtcaatttga
     2701 cctgtgatcc ttgcaaaaca ggatgtacta gcaaaccgtt ttggatatta tgctgctcat
     2761 aagatactcc tgtggctaaa agaccaacac caactgaagt aactactggt acttgttcac
     2821 cggtcgcaac aaaagcggat tccccactca tgactttaac ggattgactg cgctgcactt
     2881 cgtattgtgg ttgtgtgctg taaacgacat cattcccatc ctgtgagctt aaccattcag
     2941 gatccccttg ataaacagta atattaaaaa caacaggagg aacatctatt ttatgtaata
     3001 cgtttctgat ttgcgttaaa gtttggggac tcacattaac aattaatgtt tgccctgaac
     3061 cacttacttt ctcatcagtc tgcaataatg gttggattaa ctgaataacc ttatctgctt
     3121 gcaaataatt caattcgata accttggtta tcatctgctg ggaaaaagcg ctgccagaaa
     3181 ttaataatat cactaatagc catcgcttaa tcatagaaac actcccgttg tttcaattta
     3241 attatagaat cctttattga attgtagcac taataggact cacaaggtta caaatagccc
     3301 cctttaatcc ctaatctgta ttcactgtag ataagtagta gttttgttcc tcaaaaagtt
     3361 taggtataat cctcaaaatc tgttatccga gatcaaaaat tatgggtagt atattcaata
     3421 tgttcgggcc atcaccaata aagcccattg aacaacacat acgtaaagca catcagtgtg
     3481 ccaagcaatt gtaccctttt tttgaagcgg tgctcaaaaa cgattgggat actgcaaaca
     3541 agatcaagga taaaatcatc tctattgaaa aagaagcgga tctaattaaa cgtgatttgc
     3601 gccttcatct acctactggc ttatttttac cagtcgcccg cacagacata ttagaactgc
     3661 tcagtgccca agatagaatt gccaataaag cagaagatat agctgcttta atcataagca
     3721 gacaaatgag catacctaaa aaattaattc cgtcttttat gcctttttta aaccgatgtc
     3781 ttgatgcatc taaacaagcc tgtacagcga ttaatgaatt agatgaattg cttgaaaccg
     3841 gattcagggg cagcgaagtc aaaatcgttg aagaaatgat agtaaaatta gacgaaattg
     3901 aacatgactg tgatgaaaga ctggcagaca ttaggcacaa aatcttcgaa ttagaaaagg
     3961 aactcccagc tatagatgta atttttctat acaaactggt ccaatggatt ggcgaattgg
     4021 ctgaccatgc ccagacagtt ggtggtcgac ttcaaattct aattgcccgg tagttatcac
     4081 tcatggatta ttcaatttac ctactttttg ctgctcttat tctttgtttt cttatgacct
     4141 ggggggtcgg tgccaatgat ttggctaatg tcatgagtac cactatgggc tcaaaggcag
     4201 ttaccgtccg tcaagccatg cttatcgcta tcattttcga atttgcaggc gcttttttag
     4261 ggggtgaagg tgttactgaa accatgcgcg atgggattat caataccagt caactttcta
     4321 atgaaccatt aatcctcatc gaaggcatgc tgtgcgtttt gtttgcctgc acaatatgga
     4381 tgaatcttgc gagttatctg ggggtccctg tttcgataac caacgcatta gtaggctcca
     4441 tggttggttt tggcactata gtcttgggtc ctgacgcaat acattggaac caggtagcgc
     4501 gaatcgctat tggatgggtt tcatctccgt tgatttcagg gattacggcc tacgctttgt
     4561 tcattagtat acagcaaacc atatttgtaa aaagtaaccc gttgactaaa gccaagctat
     4621 atattcctat ttatcttttt ttgattggtt ttatattatc ttttatcact gtatttaaag
     4681 gacttaacca ttttgatatt cacttgaatt taaagcaaga tttggcggtg actctggcca
     4741 ccagtataat aattacaatt ctgggtatga tctttattaa gcgtattcct gaataccata
     4801 aaataagacg cagggaacga tttattcagg tagagaagta ttttgctgtg ctcatggcaa
     4861 tgacggcatg cgctatggct ttcgctcatg gctccaacga tgttgccctg gctgttggac
     4921 cattaagtat tgtgcacagc ctggtcatgc attccaatca aatatttgac gcagataact
     4981 acccttcctg gattatttta ttaggttgcg tcggagtagt aactggtttc cttatgtatg
     5041 gaagaaaagt cattgaaacc gtaggaagtt ctatcactgc tttaacgccc agccgtgcat
     5101 ttgctgcaac actttctgca gcaactaccg tagttgttgc aaccagcacc ggaatccctg
     5161 tttcggcgac ccaaactctg gttggtgccg ttttaggtgt aggtttggca agaggtatcg
     5221 gcgctttgaa tctcattgta atccgtaata tctttatgtc atgggtactg acattacctg
     5281 cagcctccat gctaacgatc ttatcctata aacttttaca tgctttgttg ggataatcct
     5341 cattgcctat caggctcatc ataggcaatg gattgcctaa atttcaacgc ctactgtaat
     5401 atgaaaatga attgaatatc ctttaaaata taaattcgca ggcaaaccat gtgcttttct
     5461 c
//
LOCUS       DBNCWK010000164         5460 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.165, whole genome shotgun sequence.
ACCESSION   DBNCWK010000164 DBNCWK010000000
VERSION     DBNCWK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.165"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            141..845
                     /locus_tag="AC06IO_002628"
     CDS             141..845
                     /locus_tag="AC06IO_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIO9875795.1"
                     /translation="MRNIIKQSKTSTVLEKIRMDLITGYYSPGQKLQMQQLKERYKVG
                     YSPLREALSRLVSQGLVHLEDLCGFSVPNLSLEELYDLYSVRILIETKALELSIQHGD
                     EHWEAEVIACWYRYAKYLNLDTNQPINPFEWNEFQKEFTFTLSKACQSPWLLKIQDML
                     FDHASRYRFLCLGKHFQDKKIIDEFRQECEELVSAVLARDKEKALKISQEGWENSLEI
                     MASIIKSSGIGTLKND"
     gene            838..2397
                     /locus_tag="AC06IO_002629"
     CDS             838..2397
                     /locus_tag="AC06IO_002629"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIO9875796.1"
                     /translation="MTNSKKYLLIPSIPTPNGRLHLGHIGGPFLSADILARYLRTRGH
                     SAWIISGTDSFESYAAGKAEEEHRSPEETCNYYHPLIEEDLRLMGIQMDLFINPLKAP
                     WKYLYQHWHHNIYNQLINNGTTLVIKEKFPWDENNQRYLSGYWLKGDCPICRKKTASY
                     FCENCGAHFRPEELIQEDAFNQKEVENIFLKLPNGINLSSKGVSKEIQAIFHNYLERQ
                     NQILRLTTFCDWGLADPNDKTRTLFSYGFVFAYFLMIGEIAGQLIGINRNAFASDSDV
                     ITISSFGIDNSIPFLSSSIGISSACPQYKPFDYYLVNYFYYLDGNKFSTSRQHMIGVE
                     DIITCKELSSDLVRLFLAAIDVRQQTGDFLTDEFIYYYNQTVDWLEELVITDIQNIAP
                     PTVCDDFFKDELNELLKTQECALQPENYLPHLAVNAINHWIIQGKQLNQDSKHYFWWL
                     KGLSLVIYPFMPNLGQTIWESLGYENSPRIKDFFALPLHSIQKNVPINRNRISQELMF
                     IKEEHDDEIIA"
     gene            2375..3712
                     /locus_tag="AC06IO_002630"
     CDS             2375..3712
                     /locus_tag="AC06IO_002630"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine N(6)-hydroxylase/L-ornithine
                     N(5)-oxygenase family protein"
                     /protein_id="HIO9875797.1"
                     /translation="MMTKSLRNTEVVDVIGVGIGPSNLSLAALLSPIKNISSCFYDRN
                     DSFSWHPGMLLPDSEIQVNYLNDLVTLVDPTNPFSFIAYLAKHKRLYRFINAQFKYVL
                     RSEFSQYLNWVSRSLSNLFFNEEVIDVCFDHSHFSMQTTKRIIKGKHLVLGNGLTARI
                     PSCAKSCLGKTVFHNSEYLHKSPNWYGKSIAIIGGGQSGAEIVHSILSHSDGLPEKLT
                     WISRRNQLLPMDTSPFVNEFFTPNYTNHFYQFTSETKSRLLQEQLLASDGISEDLLRT
                     IYQKLYRIEFLENKGQFFQFLAKSQIINIERAFGTHQFILVVEDLESENKQIIPADII
                     ILCTGYEWTFPPYLSSLSNQIPLHNNRFYINHDFSIQWDGPNQNRIYVQNGARHSHGI
                     ADPNLNIMAWRSATIINSIIQKPIYDLSSESSALSWEPEIISQNQECKYASIA"
     gene            3696..4046
                     /locus_tag="AC06IO_002631"
     CDS             3696..4046
                     /locus_tag="AC06IO_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIO9875798.1"
                     /translation="MLQSPNLISHPKPQLNFSTGEEIAPGVVVNPIDFSFNQIKPPFN
                     STVFHLSPGSQTTLDSHREKEIWIVLEGSGILIYEGLSFILKSQEAFFFPPFKTHQAV
                     NNTENLLFICSIYW"
     gene            complement(4179..4802)
                     /locus_tag="AC06IO_002632"
     CDS             complement(4179..4802)
                     /locus_tag="AC06IO_002632"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIO9875799.1"
                     /translation="MYTLYSFGTPNGFKPTIMLEELAQPYTIKLIDITHGEQFDPDFL
                     KISPNNKIPVLHDQTNDFYLMESVAILQYLAEKHRQFLPETTKEKYAIIQWCYFQAAH
                     IGPMLGQFGHFHRYAQEQVPYAMKRYADESMRLLGVMEKQLAKTSFIGGSNYTIADMA
                     IWPWIWCFQFVYEQTIDEKLFPCLMSWYQRVSERPAVKETLASYGRL"
     gene            complement(4838..5257)
                     /locus_tag="AC06IO_002633"
     CDS             complement(4838..5257)
                     /locus_tag="AC06IO_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HIO9875800.1"
                     /translation="MKALYTTIAKTHGGRNGHVETTDGLLKLDLAMPRELGGEGGATN
                     PEQLFAAGYAACFESAIRHVANMQKISLEDVSMTSEVSLYATPEKGFKLGVALHAYIT
                     GLNQNEAEALVAKAHEVCPYSNAIRGNVEVKLSVSVK"
BASE COUNT         1770 a         1048 c          954 g         1688 t
ORIGIN      
        1 accattttat ttgtaataaa aatccatctc aaattagtca tcactttttt tcagacttaa
       61 aatttatttt cgaatattta caaattttcg aaaataatga atacttatct cttatttaac
      121 ttgcaacgtt gaaaaccaag atgcgaaaca tcataaaaca atccaaaaca agtacagttt
      181 tggaaaaaat aagaatggat ttaattaccg gttattatag ccccggtcaa aaacttcaaa
      241 tgcaacaatt aaaagaaaga tacaaggtag gttatagccc tttacgagaa gccttatccc
      301 gtttagtttc acaaggatta gttcatcttg aagatctctg tggattttct gtacccaatc
      361 tatcattaga ggaactatat gatctttatt ccgtaagaat tctaatagaa acaaaagctt
      421 tagagctgtc tatacaacat ggcgatgaac attgggaagc agaggttatt gcctgttggt
      481 acaggtatgc caaatatctt aacctggata caaatcaacc catcaaccca ttcgaatgga
      541 atgagtttca aaaagaattt acatttaccc tgagcaaagc ctgtcaatct ccctggctac
      601 tcaaaataca agatatgtta tttgaccatg cctcacgcta tcgtttttta tgcctgggaa
      661 aacattttca ggataaaaaa attatcgatg aatttcgtca ggaatgtgaa gaacttgtct
      721 cggcagtatt agcccgagat aaagaaaaag cgctgaaaat atcacaagaa ggctgggaaa
      781 attctctcga aatcatggcg agcattataa aaagctcagg cattgggaca ttaaaaaatg
      841 actaatagca aaaagtattt acttatcccc tcaatcccga ctcccaatgg tcgcttgcat
      901 ttaggtcata ttggcggtcc cttcttgagt gctgatattt tagcaagata tttaaggaca
      961 cgcggacatt cagcctggat tatttctgga acagatagct ttgaatccta tgccgcagga
     1021 aaagcagaag aagaacatcg cagcccggaa gaaacatgca attattacca tccattaatt
     1081 gaagaggatt taagattaat gggtatccaa atggatctct tcattaatcc gctaaaagca
     1141 ccgtggaaat atctctacca gcactggcac cacaacattt acaatcagct aattaataat
     1201 ggaactactc tagtcattaa agagaagttt ccctgggatg agaacaatca acggtattta
     1261 tcgggttatt ggctcaaagg cgattgtcca atttgcagga aaaaaacagc cagttatttt
     1321 tgtgagaatt gcggagcgca ttttcgtcct gaagaactga ttcaggagga tgcttttaat
     1381 cagaaagaag tagaaaatat ttttttaaag ctgccgaacg gcatcaattt aagttccaaa
     1441 ggcgtcagca aagaaattca agccattttt cataactatt tagaacgaca aaaccaaatc
     1501 ttgagattaa caactttttg tgattggggt ttggctgatc ctaatgataa gacaagaaca
     1561 ttatttagtt atggttttgt atttgcctat ttcttaatga ttggggaaat cgctggtcaa
     1621 ttaatcggta tcaatagaaa tgcttttgcc tccgattcag acgtaatcac tatatccagt
     1681 tttggtatcg ataattcaat acctttttta agtagctcca taggtattag ttctgcttgt
     1741 cctcaataca agccctttga ttattatctt gttaattatt tttattattt ggatgggaac
     1801 aaattttcca ctagtcggca acatatgatc ggagtagagg atattatcac ctgcaaggaa
     1861 ctttcaagtg atcttgtccg acttttcctt gctgcaattg acgtgcgcca gcaaaccggc
     1921 gactttctca ccgatgaatt tatttattat tataatcaaa ccgttgactg gcttgaggaa
     1981 ttggtcataa cggacataca aaacatagct ccaccaacag tttgtgatga tttttttaaa
     2041 gatgagttaa atgaactttt gaagactcaa gagtgtgcct tgcagcctga aaattactta
     2101 ccacatctcg cagtaaatgc aattaatcat tggatcattc aaggaaaaca actgaatcag
     2161 gactcaaagc actatttttg gtggttaaaa ggattaagtc tggtgattta tccttttatg
     2221 cctaatcttg gacagactat ttgggaatcc ttagggtatg aaaactcacc aaggataaaa
     2281 gatttttttg ctctccccct gcattctata caaaaaaatg tgccaataaa tcgaaatcga
     2341 atttctcaag aattaatgtt tatcaaggag gagcatgatg acgaaatcat tgcgtaatac
     2401 tgaggttgtt gatgtaatcg gagttggcat tggaccctcc aatttaagtc tcgctgcttt
     2461 actatcaccg ataaaaaaca tttccagttg tttttatgat cgcaatgatt ccttttcatg
     2521 gcaccctggt atgcttcttc cagacagtga gattcaagtc aattatttaa atgatttggt
     2581 aaccctggtt gatcctacta accccttttc ttttattgct taccttgcaa aacataaacg
     2641 cctatatcga tttatcaatg cacaatttaa atacgtttta cgttctgaat ttagccaata
     2701 tttaaattgg gtcagtcgat ctttatccaa cttatttttt aatgaggaag tgattgacgt
     2761 ttgttttgat cattcgcatt tttcaatgca aaccacaaaa agaataataa aaggaaaaca
     2821 cctcgttttg ggtaatggtt taacagccag gataccctca tgcgccaaat cctgtctggg
     2881 aaaaacagta tttcataatt cagagtattt gcataaatcg cctaattggt atgggaaaag
     2941 tattgcgata atcggtggag gtcaaagtgg cgctgaaatt gtacactcca ttttatccca
     3001 tagtgatgga ttacccgaaa aattaacctg gatctcaaga aggaatcagc tactaccaat
     3061 ggacacctct ccttttgtta atgagttttt tactccgaat tacactaatc atttttacca
     3121 gttcaccagt gaaacaaaat cacgcttatt acaagaacag ctgttagcga gtgatggtat
     3181 ttctgaagat ctcttaagaa ctatctatca aaagttatat cgaattgagt ttttagaaaa
     3241 taaaggccag ttttttcaat ttttagcaaa gtctcaaatt attaatatag agagagcctt
     3301 tggaactcat cagtttatct tggtcgtaga ggatcttgaa tctgagaaca agcaaataat
     3361 ccctgcagac atcattattt tatgcacagg ttatgaatgg acttttcctc catatcttag
     3421 ctcactatcc aaccaaatac ccttacataa taacaggttt tatatcaatc atgatttttc
     3481 catccagtgg gatggcccaa accaaaatcg catttacgta caaaatggag cccgtcattc
     3541 tcatggcatt gcagatccca atttaaacat tatggcttgg cgcagtgcga caattatcaa
     3601 tagcattatt caaaaaccta tctacgatct cagtagtgaa agtagcgcct taagttggga
     3661 gcccgagatt atctctcaaa atcaggaatg taaatatgct tcaatcgcct aacctaatca
     3721 gtcaccctaa accacaatta aatttttcaa ctggagaaga aattgctcct ggcgtagtag
     3781 tcaaccctat tgattttagt tttaatcaga taaaacctcc ctttaatagt accgtatttc
     3841 atttatcacc tggtagtcaa accacattag actctcatcg ggaaaaagaa atttggattg
     3901 tattagaagg cagtggcatc ctgatttatg agggattatc gtttatcctc aagtcacaag
     3961 aggcattctt ttttccaccg ttcaagactc atcaagctgt gaacaataca gaaaacttgc
     4021 ttttcatttg ttctatttat tggtaagtct atgatgaccc aatctattga cacaatcacc
     4081 aagtaaagca tcccaatggg caccattacg gcaattggga gcttttaatt atcagataag
     4141 tgcaactaca tcactaacta tctaatggat attcatgatt atagccgccc ataagaggct
     4201 agagtctctt taacggcagg tctttcacta actcgttgat accaggacat taaacaggga
     4261 aacagtttct catcaatagt ctgttcatat acgaattgaa aacaccaaat ccagggccag
     4321 atagccatat cggcaatagt ataatttgat ccgccaatga atgacgtttt ggccagttgc
     4381 ttttccataa cccctagaag tcgcatggat tcatcagcat atcgcttcat ggcatagggt
     4441 acttgctctt gagcataacg atgaaaatga ccaaattgcc ctaacatagg gcctatgtgt
     4501 gctgcctgga aataacacca ttggataatg gcatatttct cctttgtcgt ttcaggcaaa
     4561 aactgtctat gtttttcagc caaatattgc aaaatagcaa cactttccat taggtaaaaa
     4621 tcattggttt gatcatgcaa aaccggtatc ttattgtttg gtgaaatttt taaaaaatca
     4681 ggatcaaatt gttcaccatg agtaatatca atcagtttta tggtgtaagg ctgtgccaac
     4741 tcttccaaca taatagtggg tttaaatccg ttgggcgtcc cgaatgaata aagtgtatac
     4801 attaagatat ccttatttaa ttgatggaag ttatctatca cttgacacta acactcaatt
     4861 tcacttcaac attaccacgt attgcattgg aataaggaca aacctcatgc gcttttgcaa
     4921 ctaatgcttc ggcctcattc tgatttaggc ctgttatata ggcgtgtaaa gcaactccaa
     4981 gtttaaaacc cttctctggg gtagcatata aagagacctc ggaagtcatg gaaacatcct
     5041 ctaaagaaat tttttgcata ttggcaacat gacgaatggc actctcaaaa caagcagcat
     5101 atcccgcggc aaataattgc tctggattag tggcaccacc ctccccccct agctctctcg
     5161 gcattgccaa atcaagtttt aatagcccat ctgtagtttc tacatggccg tttcgtccac
     5221 catgggtctt ggcaattgtt gtatacaatg ctttcataat tttatattcc ttaaaatggt
     5281 aagaaattaa aattatttag tattttgctg tttgtcaaac agaaaggaag aaatttcatg
     5341 ccgaacttta attcgtgcct ggaaagaaaa cagccttgat aagcaaagaa aagacgatac
     5401 aaacttaaat tagttacaaa ttgagaaaat cagtcttaat aatcggaaaa catataacca
//
LOCUS       DBNCWK010000165         5419 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.166, whole genome shotgun sequence.
ACCESSION   DBNCWK010000165 DBNCWK010000000
VERSION     DBNCWK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.166"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            109..306
                     /locus_tag="AC06IO_002634"
     CDS             109..306
                     /locus_tag="AC06IO_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875801.1"
                     /translation="MLKTLGGATRTAALDTARATATTTVVLGTMYFWDRVAKNYGLYD
                     KENSKPINLDLTNDEYMRPKV"
     gene            complement(395..1297)
                     /locus_tag="AC06IO_002635"
     CDS             complement(395..1297)
                     /locus_tag="AC06IO_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIO9875802.1"
                     /translation="MKKIILYLFVAIGVYAVLLFAYNMLINRAGQTLYGGAPAMGSAY
                     PTVEKVNAVPGGSGDSNNNPSASEQVMKGMIIRNANITLEVNDPTKVIDEIMQMAERE
                     GGYVVNSNLRQDSFYVGGNIAQISIRIPSNRLNNVLQNLKSLAVKVIEESITSEDITR
                     QYVDLESELKNYRVAKDQLNKIMQGAKNTSDVLAVYQQLSETQRKIDVIEGQIKYYKE
                     SIAYSLVTIYLQIDPAIKNTQNSTWQISEVVMKSYQALIDNLRQFTYGVIEFVILFVP
                     MILLWGIIALFVFWIGRVIYSRFK"
     gene            1467..2078
                     /locus_tag="AC06IO_002636"
     CDS             1467..2078
                     /locus_tag="AC06IO_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIO9875803.1"
                     /translation="MKKIAPTLKNEIINTAIDIAQDTSWEKVRLIDIAKALNIDLNTI
                     RYFFAEKEQIIDGCFDRADEAMLNASSRPKFDLLTAKEKLHDLLMTWLSTFEGQQKVI
                     RQMIFHKLEPGHLHLQLPAIKRISQTVQWWREAAGLRETFIQRAIQETGLTIIYLATF
                     SYWLLDNSTEHLATRLFLENKLNMAKNLSNFWGFTYYAPSKIS"
     gene            2056..3123
                     /locus_tag="AC06IO_002637"
     CDS             2056..3123
                     /locus_tag="AC06IO_002637"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIO9875804.1"
                     /translation="MHHLKLVERLGIQFPIIQAPMAGGATTPELVAAVSNSGGLGSLG
                     AGYMRPDKIRQAIIKIRQLTSKPFAVNLFIPEAHHATPEQIQNACDDINLCCTELNIE
                     ISPVSKPYSLPFVDQMQILIEEKIPVFSYAFGTLEPMWIKQLKKNGTFLIGTATTIHE
                     ARILEASGIDAIVAQGSEAGGHRGTFIGNAEEDFIQLSELIPQLVETIRVPVIAAGGI
                     MNGKGIISAINSGASGVQMGTAFLSCFETGISYKYKQTLLSQQQDNTVLTRAFSGKLA
                     RGIRNKFITCMDNRKINILDYPIQNALTQVMRQKAREQDNIDFMSLWAGQSAHLCRSM
                     SASDLISTLVIEAKNVFNEGV"
     gene            3258..3638
                     /locus_tag="AC06IO_002638"
     CDS             3258..3638
                     /locus_tag="AC06IO_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875805.1"
                     /translation="MFRYFKQGWHGDLKFSEVLFGSGGAYFLLEGGLAYIGFYILFAI
                     LLITSKPLSLDNILALALFSYGIVLYIWLIKAFWGSANHCSNKISAVLIRTFTILLPL
                     ISIALFFLIIIYYLVTAITDALSG"
     gene            3692..4819
                     /gene="cfa"
                     /locus_tag="AC06IO_002639"
     CDS             3692..4819
                     /gene="cfa"
                     /locus_tag="AC06IO_002639"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HIO9875806.1"
                     /translation="MDIKKNKAKTFVNYMLQLANININGQTPWDIQIYNDEFYSRLLR
                     DADLGLGESYMDGWWDCQRIDLFISKLINANLESKIKINFKLAFNVFLSKILNLQTQK
                     RSLHVGRQHYDRGNDLFQIMLDSNMNYTCGYWRKAENLEQAQLDKLYLTCRKLCLKPG
                     MKLLDIGCGWGGLAKYAAENYGVSVVGITISQQQYELAKTRCAHLPVEIRFQDYRDLN
                     EKFDRIVSLGMFEHVGYKNYRKYMEIVHQCLNDDGLFLLHTIGSNESVTKATPWISKY
                     IFPNGMIPSIMQIGQASEKLFVMEDWHNFGADYYKTLMAWHENFNQGWNQIKSQYSEK
                     FFRMWNYYLLSCAGAFDARMLQLWQIVFSKKGIQGGYSAPR"
     gene            4984..5310
                     /locus_tag="AC06IO_002640"
     CDS             4984..5310
                     /locus_tag="AC06IO_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875807.1"
                     /translation="MPKNNEPSGEELKKAGIVVGITNLLLWPFYYADSRWGLFASIFA
                     TSAVLYASHEVGESKRSVENSVARLGLFQQRGTSNAEIENAFNNTVTGAAAIYDWLVP
                     GSNGPK"
BASE COUNT         1740 a          977 c         1006 g         1696 t
ORIGIN      
        1 aatgtattgc attaggcaga aattcattta gaatgaatga tgaataatta gcaggattat
       61 tattaactaa tcaggacggt aaatatgtta tcattctttg ggaaatgtat gttaaagacc
      121 ttaggcggag caaccaggac agcggctctt gatactgcga gagcaacagc aacaacaacg
      181 gttgttctag gcactatgta cttctgggac agagtagcta aaaattacgg actatatgac
      241 aaggaaaatt ccaagccaat aaaccttgat ctgactaatg acgaatatat gcgccctaaa
      301 gtttaaacta aaagattatt tttgctccca actatcctgt tgggagtatt tagctctcac
      361 ttaaatcaaa atctgaaata cgctgcaaat tctattattt aaaacgagaa taaattaccc
      421 tacctatcca aaatacgaat aaagcaataa ttccccacag taagatcatt gggacaaaca
      481 agataacaaa ttcgatgact ccataagtaa actgccgtaa attatctatt aaagcctggt
      541 aagatttcat cacaacttca gatatttgcc atgtggaatt ttgtgtattt tttatcgcag
      601 gatcaatctg caggtaaatt gtaactagtg aatatgcgat agattccttg taatacttaa
      661 tctgcccttc tatcacatca atttttctct gcgtttcact cagttgttga tagacagcaa
      721 gtacatcaga agtatttttt gcaccttgca taattttatt taattgatct ttagcaaccc
      781 tgtaattttt cagctcactt tcaagatcaa cgtattgccg ggtaatgtcc tcactcgtga
      841 tcgattcctc aattacttta acagccaaag attttaaatt ttgcaataca ttgtttaaac
      901 gattacttgg tattctaatg ctgatttgag cgatattccc accaacatag aaactatcct
      961 gtcttaagtt agagtttact acatatcctc cctctcgctc agccatctgc ataatttcat
     1021 caataacttt agtcggatca ttgacttcca gagtgatatt cgcattacga attatcattc
     1081 ctttcatgac ttgctctgaa gcagaaggat tattgttgct atcaccagaa ccacctggta
     1141 ccgcattgac tttttcaaca gtgggatagg cactccccat tgctggagcg cccccatata
     1201 atgtttgtcc ggctcgattt atcagcatgt tgtacgcaaa tagaaggaca gcgtaaactc
     1261 caatagcgac aaacaagtat aaaatgattt tcttcatttt taattcaata gtccgatatt
     1321 ataaaaataa gtgtggcata gactaattga aataaccaat agcatttttt gatttaactt
     1381 atagttaatt taactaccaa aaccatcaaa aattaatcct gatcataaaa ttagtaataa
     1441 actaataaga gacagggagg tatttcatga aaaaaatagc accaacacta aaaaatgaga
     1501 tcatcaatac agcaatagac attgcccagg atacctcttg ggaaaaagtg aggctgattg
     1561 atatcgccaa agcattaaat atcgatttaa atacgatccg atattttttt gcggaaaaag
     1621 agcaaatcat tgatggctgt tttgatcgtg ctgatgaagc catgctcaat gcgtcctcca
     1681 gaccaaaatt tgatttactc actgccaaag aaaaactgca tgatttatta atgacttggt
     1741 taagtacttt cgaaggccag caaaaagtaa tccggcaaat gatatttcat aaattagagc
     1801 ccggtcatct tcatcttcaa ttgcctgcaa tcaaacgcat cagccaaact gttcaatggt
     1861 ggcgtgaagc agctggtctt cgagaaacct ttattcaacg tgccatccag gaaactggat
     1921 taaccatcat ttatctggcc acctttagtt attggttatt agataactcc acagagcatc
     1981 ttgcaactcg tctttttttg gaaaacaaat taaatatggc aaaaaattta tccaattttt
     2041 ggggttttac ttactatgca ccatctaaaa ttagttgaga gacttggtat tcaatttcct
     2101 atcattcaag cacctatggc cggaggagct actactcctg aattggttgc agcggtttct
     2161 aacagtggtg gtttgggctc tctgggcgca gggtatatga gacctgacaa gatcagacaa
     2221 gccattataa aaattcgtca attaacaagc aaaccttttg ctgttaatct ttttataccc
     2281 gaagcacatc atgcaacacc agaacaaatt caaaacgctt gtgatgatat caatttgtgc
     2341 tgcactgaac taaatattga aatcagtccc gtgagtaagc cctactccct gccttttgtt
     2401 gaccaaatgc aaatcctgat agaggaaaaa atccctgtct ttagctatgc attcggtaca
     2461 ttagaaccga tgtggatcaa acaattgaag aaaaatggaa cctttctgat aggaacggca
     2521 accactatac acgaagcaag aatacttgaa gcaagtggga ttgatgccat tgttgcacaa
     2581 ggaagtgaag caggagggca tcgaggtact tttatcggca atgctgagga agattttatt
     2641 caactatctg aactgatacc tcaactagtt gagacaataa gagtgccagt gatagccgcc
     2701 gggggaatta tgaatggtaa aggtattatc tctgccataa attcaggtgc ctctggtgta
     2761 caaatgggaa ccgctttttt aagttgcttt gaaactggta tctcttataa atacaaacaa
     2821 acactattat cacaacaaca agataatact gtgctaacac gtgctttttc tggaaagctt
     2881 gctcgtggaa ttcgaaataa attcattact tgcatggata atcggaaaat aaatatcctt
     2941 gactacccca ttcaaaatgc attaacccag gtaatgagac aaaaagccag agaacaggat
     3001 aatattgatt ttatgtcgtt atgggctgga caatcggctc atttatgccg atctatgtcg
     3061 gcaagtgact taataagtac tttagttatt gaggcaaaaa atgtttttaa tgagggagtt
     3121 tagataaact aaatgaccga attacaaact ttttttaaca aagagactgg gggtttaagt
     3181 aagtcctgaa aaattctttc atccattgtc ctggcttggc ttataataaa aattataaat
     3241 aacataacag gatccgtatg tttcgttact ttaaacaggg atggcatgga gatctgaaat
     3301 tttctgaagt tctgtttggt tcaggtggtg cttatttttt gcttgaaggc ggcctggcgt
     3361 atattggatt ctatatttta tttgccattt tgttaataac atctaaacca ttatccctgg
     3421 ataatatatt agccttggct ctcttctcct atgggatagt cttatatatc tggttaatta
     3481 aagcattttg gggatcagcg aatcattgct caaataaaat ttcagccgtt ttgattcgta
     3541 cctttaccat tcttttgccc ttgatctcga ttgcgttgtt ttttctgatt attatttatt
     3601 atcttgttac agccataaca gacgctttga gtggctgatg atgttgcatt gattgaatgt
     3661 ttcaacatcc tttataaaaa gggagtttga tatggatatt aaaaaaaaca aagcaaaaac
     3721 atttgttaat tacatgcttc agctagccaa tatcaatatc aatggccaaa caccttggga
     3781 tatacaaatt tataatgatg agttctattc cagattattg cgtgatgccg atttgggtct
     3841 gggtgaaagt tatatggacg gttggtggga ttgtcaacga attgatttat ttatttcaaa
     3901 actgattaac gcaaatcttg agtccaaaat taaaattaat tttaaattgg catttaatgt
     3961 ctttttatca aaaatattga acctgcaaac ccaaaaacgt tctctgcatg ttggtagaca
     4021 acattatgat agaggaaatg atctgtttca gattatgctt gatagcaaca tgaactacac
     4081 ttgcggttat tggagaaagg ctgaaaattt ggagcaagcc caactcgaca agctctatct
     4141 gacctgcaga aaactctgcc ttaaacccgg tatgaaattg ctggatatcg gatgtggatg
     4201 gggaggactt gcaaaatatg ctgctgaaaa ctatggggta tcagtagttg gcataacgat
     4261 atcacagcaa caatacgagt tagctaaaac gcgctgtgct catttgcctg ttgaaatccg
     4321 atttcaggat tatagagact taaatgaaaa attcgatcgt atagtctcct tagggatgtt
     4381 tgagcatgtt ggttacaaga attaccgaaa atacatggag attgtccatc aatgtttaaa
     4441 tgatgatggt ttgttcttat tacacactat aggttccaat gaaagtgtca caaaagccac
     4501 accatggatt tcaaaatata tttttcccaa tggaatgatt ccatccatca tgcaaatagg
     4561 ccaagcttcc gaaaaattat tcgttatgga agattggcat aattttggag ctgattatta
     4621 caaaacatta atggcatggc atgagaattt caatcaggga tggaaccaaa ttaaatccca
     4681 atatagcgaa aaattctttc gtatgtggaa ttattatttg ctctcttgtg ctggagcttt
     4741 tgatgccaga atgttgcaat tatggcagat tgttttttca aagaaaggaa ttcaaggagg
     4801 ttactctgcg ccgaggtaaa tggtttaaac aatttattcc gcatcgtaat accttcacca
     4861 gcaaaccagg ttttccatgc catgaagtta gcaagattat tcatatatcc cgttaatttt
     4921 gcttgcaatt tttattatct ggaatatagt ttgaactgaa attttgtcta aaaaggaatt
     4981 aatatgccaa aaaataatga accaagtgga gaagagctca aaaaagcagg gatagtggta
     5041 ggaataacaa acctgctttt atggccattt tattatgccg actcgagatg ggggttgttt
     5101 gcatccatat tcgctacttc cgcagtactt tatgcttctc acgaagtcgg agaaagtaaa
     5161 cggtccgtcg aaaattcagt ggcgcgactt ggtctcttcc agcagcgtgg aaccagcaat
     5221 gctgaaattg aaaatgcttt taataatacg gtcacaggtg ccgctgcgat atatgattgg
     5281 cttgttcctg gctctaatgg ccccaaataa taagcatctt gtcttataat aaattatatt
     5341 tttgggaagt attattttat ccaaaaccac atcgttctgg agattggtca agatctccag
     5401 aaatctgttg tctttactt
//
LOCUS       DBNCWK010000166         5328 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.167, whole genome shotgun sequence.
ACCESSION   DBNCWK010000166 DBNCWK010000000
VERSION     DBNCWK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.167"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            291..1718
                     /locus_tag="AC06IO_002641"
     CDS             291..1718
                     /locus_tag="AC06IO_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIO9875808.1"
                     /translation="MAQVINTNVASLTAQRNLGVSGNMMQTSIQRLSSGLRINSAKDD
                     AAGLAISQRMTAQIRGMNQAVRNANDGISLSQVAEGAMQETTNILQRMRELSVQAANS
                     TNNSSDRASIQSEISQLKSELERIAQNTEFNGQRILDGSFSGASFQVGANSNQTINFS
                     IGSIKASSIGGIATATGTEVAGAAATDITIAIGGGAATSINSSANFTGALNGQDATSA
                     YAKAAAINDAGIGGLSVTASTSGTQAVGAIGGTAGDTYNLTINGVAIYTNLDVATALT
                     NSDLRDAINGVSNQTGVVASLNGGNMTLTAADGRNITVTESGTGFTAGTDGLTVTGGA
                     FDGALRGTLSISAVDTIAIGGTVANVGLSANISKDTVGIDSLDVSTASGAQTAIKRID
                     AALNSVNSNRANMGALQNRFESTIANLQNVSDNLSAARSRIQDADYAAEMASLTKNQI
                     LQQAGTAMLAQANSLPQSVLSLLGR"
     gene            1801..2082
                     /locus_tag="AC06IO_002642"
     CDS             1801..2082
                     /locus_tag="AC06IO_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875809.1"
                     /translation="MSIEPVPSVNRAAQIDQIKIVEEASKPEIKNMVYDSALDAETKQ
                     ALDKATGLLQTIITEKISDKVLRKMPSDEYLQLLSLLDDIISGSIDKHV"
     gene            2145..3770
                     /gene="fliD"
                     /locus_tag="AC06IO_002643"
     CDS             2145..3770
                     /gene="fliD"
                     /locus_tag="AC06IO_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HIO9875810.1"
                     /translation="MELSSPGVGSGLDVKNLVDAIVTAEITPSQVRHDKQLNSVNTEL
                     SALGQLKSYLTNLQTSLAKLSSLSQFYTMKSTISDPNYFSATLGTGATKNTYQIEIQK
                     LAQQHSLASSYMTNTGSGTITIDFGTYNSDKTTFTPNPSATSVNITITPGNDSLVAVR
                     DAINNTNSGITASIVQDSQGSRLTITSSKTGENYAMKISGGVASLNYDPTMGVNSLTE
                     TVAAQNSTVKINGLTLVQSSNQLNEAITGVSIDLKKAEIGKVVTLTVDDNKDQLTGLI
                     NEFIKQYNDTMTFLTNLTGYNNETKQGGVFQGDPQFRNLKLNLNKWATTPLANQEGPI
                     RSLADLGIITNKQGLLEIKQDKFKTALDNHYKEIGALFAKTAKANDTGVNIKSINSTV
                     KSGTYDVVLTEFTPGVSMSGTIGGLSATSSDGITLTGSADLSGLSINVFSGSAGSRGQ
                     ITVTDGIAVQLSSLLDAYIEIKGDLEQRKDQLNNQVKQLNKIQNEIDIRRKSIETRYL
                     KQFTALDTLLSQLQSTSVFLSQQLANLPQLKLK"
     gene            3780..4190
                     /gene="fliS"
                     /locus_tag="AC06IO_002644"
     CDS             3780..4190
                     /gene="fliS"
                     /locus_tag="AC06IO_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HIO9875811.1"
                     /translation="MRMKNLYSQISSQYKAIELQTRIETASPHELIDLLIQGARSHIA
                     TAQGNIQRNQIKEKGEHIGKAISIIEGLKMSLNHDKGGEIAENLLQLYDYVQQILLKA
                     NLKNDEDLLAQSNMLLAEVHQAWQAINSGAGNEL"
     gene            4513..5259
                     /locus_tag="AC06IO_002645"
     CDS             4513..5259
                     /locus_tag="AC06IO_002645"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIO9875812.1"
                     /translation="MNTFKSSLAFVICLLLGMQSAQAQMRYGETLCNLPEYFCMKIKS
                     GDTWDRLFPNIEEQDIVRRINRMNIRLKPGMIIAVPKNIERLTIYDVSPFPRYIEPSG
                     EKTIYVSQKKLAWGAYDEDGELLWWGPISSGVGKCKVIGGCSTPTGSFRIIRKQDIDC
                     VSTVFPRRSNGEHGGALMPYCMHFFRGYALHGSYEVPGYRASHGCVRMFIEDARWLNE
                     EFIDLPGGGMRGTRVILDAPDTQDNPDNLQ"
BASE COUNT         1778 a         1036 c         1051 g         1463 t
ORIGIN      
        1 aaccagaacc aaatcaccct gaaaacccca attactcttt aaaaatattc aagtccatat
       61 gaggaatttc agacgcattg attatatttc aatcgatctt tctatttatt taacactcta
      121 attttattca ataatttttt gcgatttttc aaaaaagcgc tttcggaact aaagtttttt
      181 tcactatttc cgataactaa atcgggtaca gtaaattcac aacaataaat cgaatttact
      241 gaattacaaa tgtcccatac cagggattca ggtctgagga gactaaaact atggctcaag
      301 taatcaacac taatgtggct tcgcttacag cccaacgcaa tttgggtgtt tcgggcaaca
      361 tgatgcagac atcaatccag cgtttatcat caggattaag aattaacagt gccaaagatg
      421 atgcagcagg actcgccatt tctcaacgaa tgactgcaca aattcgcggg atgaatcaag
      481 ccgttagaaa tgctaacgat ggtatatccc tatcacaggt tgctgaagga gcaatgcagg
      541 aaacaaccaa tattttgcaa cgtatgcgtg agctttccgt tcaagctgcc aactcgacca
      601 ataactcatc tgatcgcgcc tcaattcaaa gcgaaatttc acaattaaaa agtgaattgg
      661 agcgtattgc tcaaaatact gaattcaatg gtcaaagaat tctcgatggt tctttctctg
      721 gcgcaagctt ccaggttggg gcgaattcaa accaaaccat caatttcagt atcggtagca
      781 taaaagcttc ttctattggt ggtattgcca cggcaacagg aacagaagta gctggtgcag
      841 cagcgacaga tattactatc gcaattggag gaggagcagc aaccagtatt aactcttctg
      901 ccaatttcac cggggcacta aacgggcaag atgctacgtc tgcctatgcc aaagcagcgg
      961 ctatcaacga tgccgggata ggaggactat cggttacggc ctctacaagc ggcacacaag
     1021 cagttggcgc aataggtgga accgcaggtg atacctacaa cttaacaatt aatggtgttg
     1081 caatatatac taacctcgat gtagcaacgg cattaaccaa ctccgatctt cgggatgcta
     1141 tcaatggtgt cagtaaccaa actggagttg tagcgtcact taacggtggt aacatgacac
     1201 tcactgctgc tgatggcaga aacattacgg taacagaaag cggtacaggt tttactgcgg
     1261 gtactgacgg tttaactgtg actggtggcg catttgatgg cgcgctacgc ggtactttat
     1321 cgataagcgc tgttgatact atcgcgattg gtggtactgt tgctaacgtt ggtttaagtg
     1381 ccaatatctc taaagacaca gtagggatag attcactgga tgtgagcaca gcgtctggcg
     1441 ctcaaacagc gattaagaga attgatgcag ctctcaatag cgtaaactct aatcgagcta
     1501 atatgggtgc tctgcaaaat cgctttgaat caacgatagc caacctgcaa aacgtatcag
     1561 acaacttatc agctgctaga agtcgtattc aagatgctga ttacgctgct gaaatggcta
     1621 gtttgaccaa aaaccaaatt ctgcaacaag caggtacagc gatgttggca caagctaata
     1681 gcctaccaca atctgtatta tcgttgttag gtcgatagga tgtcgcaatc gaagtgcagc
     1741 aacaactgca cttcgatttc tttatacctc ggttgatggg tataaaaagg agtaagaaaa
     1801 atgagtattg aacctgtacc ctcagtcaat agggcagctc aaatagatca aataaaaata
     1861 gttgaggaag cctctaagcc agaaattaaa aatatggttt atgattccgc tttggatgcc
     1921 gaaacaaaac aggcgctgga taaagcaacg ggtttgcttc aaactataat aacagagaaa
     1981 atatccgata aagtattacg taaaatgcct tctgatgaat atttgcaatt actaagcttg
     2041 cttgatgata ttattagtgg ttctatagac aagcacgttt aataatttga aatatttatt
     2101 aaacatttga ctagaacaat aacaacatag aaaagggata ctctatggaa ctctcttcac
     2161 caggcgtagg gtcaggactt gatgtaaaaa acctggtcga tgcgatagtc acggcagaaa
     2221 taaccccatc gcaagtgcgt catgataaac agttaaatag tgtcaataca gagttgtcgg
     2281 cgcttggtca attaaaaagc tatttaacta acttacaaac ttcgttagcc aagctctcca
     2341 gtcttagcca attctacact atgaaatcaa ctattagtga tccgaattac ttttccgcta
     2401 ctctggggac tggcgcgact aaaaatactt atcaaattga aatacaaaaa ctggcgcaac
     2461 aacacagtct ggcaagctct tacatgacta atactggaag cggaacaata accatagatt
     2521 ttggaaccta taacagtgat aaaaccactt ttacaccgaa tccttccgct acgtcagtca
     2581 acataaccat tacgcctggc aacgatagcc ttgttgcagt acgagatgcc attaataata
     2641 ctaattcagg aataacagcc agcatagttc aagatagcca gggctccagg ctaactatca
     2701 cttcgtcaaa aaccggcgaa aattatgcca tgaaaattag tggtggagtt gcctcattga
     2761 attatgatcc taccatggga gtgaattcat taactgaaac agtagcggca caaaacagta
     2821 ctgttaaaat taacggatta actttggttc aaagttctaa tcaattgaat gaggcaataa
     2881 ctggtgtcag cattgatcta aagaaagccg agattgggaa agttgttacc ttaactgttg
     2941 atgataataa agatcagcta acaggcttaa tcaacgagtt cataaaacaa tataatgaca
     3001 ccatgacttt tttaaccaac ttaactggtt ataacaatga aaccaaacaa ggaggagtat
     3061 ttcagggcga ccctcaattt cgcaatttaa aactcaattt aaacaagtgg gcaactaccc
     3121 ctttagctaa ccaggaagga ccaatccgct cattagccga tttgggaatc atcaccaata
     3181 aacaaggttt actggaaatc aaacaagaca agtttaaaac tgcactcgat aatcactata
     3241 aagaaatagg agcattgttt gcaaaaacag ccaaggctaa tgatactggc gtaaacatca
     3301 aatccattaa ttcaactgtc aaatcaggta cctatgatgt ggtgttgact gaatttactc
     3361 ctggagtcag catgtctgga acaataggag gcttatctgc tacatcatct gatggcatta
     3421 cattgacagg aagtgctgat ttgagtggtc tgtcgattaa tgtattttca gggtctgccg
     3481 gctcccgtgg acaaataact gttacagatg gtattgcagt ccaattaagt tccttgctgg
     3541 atgcttacat tgaaattaaa ggcgatttgg agcagagaaa agatcaactc aataatcaag
     3601 tcaagcaatt gaataaaata caaaacgaaa ttgatatcag gcgcaaatca attgaaactc
     3661 gctatttaaa acaattcact gctctggaca ctctactcag ccaattacaa agcactagtg
     3721 tttttctatc ccagcaattg gccaatttac cccaattaaa attgaaataa taggaggtaa
     3781 tgagaatgaa aaacctatat tcacaaattt ccagccaata caaagccata gagctacaaa
     3841 ctcgcataga aacagcctcc cctcatgaac ttatagattt attaattcaa ggagcccgat
     3901 ctcacattgc tactgctcag ggtaatatac agcgtaacca aattaaagaa aaaggagaac
     3961 acattggcaa agccattagc atcattgaag gtttaaaaat gagtctcaat catgataaag
     4021 gtggagaaat tgccgaaaat ttactccagt tatatgatta tgttcaacaa atactactta
     4081 aagcgaattt aaaaaatgat gaagatttat tagctcagtc taatatgcta ttagctgaag
     4141 tgcatcaagc ctggcaagcg attaactctg gtgctggtaa tgagctttaa tatttgcaat
     4201 gaactcccat gattcagcat ttgcttttta cggctttata gcaaccaaaa taagagtttt
     4261 cttgacttgg caattattaa acccagtcca tctattttta ttttcccaat gccaatagtt
     4321 taaggcttcc aaatatcaat aagatatgag gaattgtctg gaagtcttgt agttatcgaa
     4381 atttttgtac attttggtaa tcaagaatag gattataatt ttgacaagtt tgtatagatt
     4441 tatcgagttg gaatataaag tttttccatt atttgccgcc aaaacagtta agatgaataa
     4501 aataatttca ttatgaatac atttaaatca tcactggcat ttgtaatttg tctcttgctt
     4561 ggcatgcaat cggcacaggc gcaaatgcga tatggagaaa ctctctgcaa cttgcctgaa
     4621 tatttttgca tgaaaataaa aagcggggac acttgggata ggcttttccc taacatcgaa
     4681 gagcaagata ttgtcagacg cattaaccga atgaatatca gactcaaacc cgggatgatt
     4741 attgctgttc ctaaaaatat agagcgctta accatctacg atgtctcacc ctttccaaga
     4801 tatattgaac caagtggtga aaaaacaatc tatgtcagtc aaaaaaaatt ggcgtggggt
     4861 gcatatgatg aagatggtga attgctttgg tggggaccaa tatcatcagg cgttggcaaa
     4921 tgtaaagtta taggaggctg ttctacacct acaggctcat ttcgcattat tcgcaagcaa
     4981 gatattgatt gtgtttctac tgttttccca agaagatcaa atggtgaaca tggtggtgct
     5041 ctgatgcctt attgtatgca ttttttcaga ggttatgcct tgcatggaag ttatgaagtt
     5101 ccaggatata gggccagcca cggttgtgtt cgcatgttta ttgaagacgc gcgttggtta
     5161 aatgaagaat ttatcgacct cccaggtggg ggaatgagag gaacacgagt aatccttgat
     5221 gcgccagata ctcaagacaa tccagataat ctgcaataat atattgaaaa cacaatcaaa
     5281 aaaccccatc ctgatagaca aatagtggcc aatctgataa tattttat
//
LOCUS       DBNCWK010000167         5219 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.168, whole genome shotgun sequence.
ACCESSION   DBNCWK010000167 DBNCWK010000000
VERSION     DBNCWK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.168"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            3..611
                     /locus_tag="AC06IO_002646"
     CDS             3..611
                     /locus_tag="AC06IO_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875813.1"
                     /translation="MVAGINPVLASVLPSSKDAQLCTNAYNKLWHIFDSVKADHRGDP
                     AAQAAIFNVEFPKVLAEDFSLTINNLPVEPNSSETITVNVQGLPQVISVASEQITSWG
                     QHHIAGPFSFKLLSESLPYKTRVYLMTTRDIDYTNSPENNGCAAFLAQKNMVCTVKNT
                     GLTTRKAMLNSMNSHVVTTYVMPAPNCSLWIAPPVEDFPTEA"
     gene            924..3158
                     /gene="legC4"
                     /locus_tag="AC06IO_002647"
     CDS             924..3158
                     /gene="legC4"
                     /locus_tag="AC06IO_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC4"
                     /protein_id="HIO9875814.1"
                     /translation="MKPNLFYFVLTQILAESPNFKLLDEVRPVHDITLKDKKYYRLEL
                     PLANIAAEGFRLHDAHISLYEKTDPHNPNLGLSHFTAIFHDQNGQTYRMHLFLNRFDA
                     LACPATWELLDDDEQYVKVSPPENLEYFIHSVWQLGLPCLQLLRKKQKEIEAKLLADY
                     HRLNEETTALSKDLDKNRAVYLEKLDELIKTTKSLSQISESNHWLREAIYLGKTYKYV
                     SSMPESILEPVKKSDEEHKKDDKAEIVTLSPKQGAKEPKISRGNIGLAQSTLFSKSAK
                     AKRSVDVKVERDISKIKKLFAQLLKTEDKKIKAVLLIDLHWKIKDINLETEELLTKEQ
                     TQALNEIEGHANKEAKSLLERALFAGEFEYAQTLSPYYPLINNDLMVLALTQRKADLL
                     SFLVTKVGLPINSYPIKTKVKTYSNAVEYCFSEHSESLVDCFSVLIKNGASLMQPVGL
                     YKLPLAHLLLSEIPRHPLHASLEQNKNLTLNNKQFYAHLINALKSCLLSGDIEGDQKI
                     ALEESIVRYEHLKTNVKNSSSLLSPQNQALADEISDITKKLLPDAVAEAIENDEEISR
                     EKAIADKEYKELIRKVQAFYHKTGKNFAFQSLINSANQELKKELLEIDFNIDISFSEL
                     KESILENLSKERLMFSYCSELIDVQRTIMQISQGASRKNKNLKKLNARHAELIELLTD
                     LSQNTPQSMIKEANEFQDSFSQLQDLLNGLNQLEGPLGMLAGVLNQFLEGSTSESESF
                     GMKL"
     gene            complement(3212..4021)
                     /locus_tag="AC06IO_002648"
     CDS             complement(3212..4021)
                     /locus_tag="AC06IO_002648"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIO9875815.1"
                     /translation="MKIIHISDLHFGMHQPKVLNAFLQETALDKPDIILISGDLTQRG
                     LSYQYREICSFINQLPAQTLTVPGNHDIPFYNVPARFIFPFRQYKRYINPDITTTFEN
                     DCVRILGVNSVNPLQLKNGKLSHETLNRIERYFKPDDEKLNLLFFHHNFDYMEGLHKP
                     LANAQTLLSFLKQSTIHLVCTGHLHYAHLSLIAKNNGYSCLALHAGSLLCPRSKDNLN
                     SYYVIEANQKKCHITWRVFDQEHFTDRSIYFIDFNKKNASFEHLNPVTDDA"
     gene            complement(4018..4881)
                     /locus_tag="AC06IO_002649"
     CDS             complement(4018..4881)
                     /locus_tag="AC06IO_002649"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIO9875816.1"
                     /translation="MTDIGVIINNRAKNAHRMEDYLSELRAHDIDYQLYKADPQQLEK
                     NIQHCCSKYPLLLIGGGDGTVRSAAQWCSNTSVILGVLPLGTMNHFSKELNLPSTTEE
                     LIAAIVEKTTTTIDVAEVNGHIFINNSSIGFYSRLAKNRDYYTNFYNKWLTYIPSFIR
                     ALSYHPTYALLIKNKELDISIRTSFFMVSNNCYTYEFPLKFTRESFQNEQLGIYYYKR
                     SKLRLFKFLIDFLTDKEHFEIIQTRLPLEVDVLHKNKITISLDGDTLAIAPPLYYKIL
                     PKSLKLLTKKI"
BASE COUNT         1686 a          909 c         1002 g         1622 t
ORIGIN      
        1 taattgttgc cggtatcaat ccagttttag caagcgtgct tcccagttca aaagatgctc
       61 agctttgtac caatgcttat aataaacttt ggcatatttt tgacagtgtg aaggctgatc
      121 accgaggtga tccagctgct caagctgcta tatttaatgt ggaatttccc aaagtactgg
      181 ctgaagattt ttccctgacc attaataatt tacctgttga acccaatagt tctgaaacaa
      241 ttactgtgaa tgtgcaagga cttcctcaag tgatttccgt ggcttcagag caaattactt
      301 cgtggggaca acaccatata gctggtcctt tcagttttaa actcctctct gaatcattac
      361 cctacaaaac acgggtttat ctcatgacaa cccgagatat tgattacacc aattctcctg
      421 aaaataatgg ttgcgcggct ttcctggcac aaaaaaatat ggtttgcact gttaaaaata
      481 ctgggcttac gactagaaaa gctatgctta atagtatgaa ctctcacgtt gtaacaactt
      541 atgtcatgcc tgctccaaat tgcagtctat ggatagctcc tccggttgaa gattttccta
      601 ccgaagcata atttacacta ataaaatgag tttttttccc gaaaagccac ttatccaatt
      661 tccccaaagt gctatacaat ctgtcacttt ggggtgttca atgaattaag caaggagccg
      721 acattcgcag gttttacatt ctctcttact gtgatgctgt cggcttttta ttgaatcgat
      781 gcagtactta caggcatttg cttgttgtta ttcaggatgc cattccatgt tgtggacaat
      841 agcatgtgga tttaaaaaaa acatgttgat caaatttaat tcccaatgta tccttggctc
      901 attttaatga gcagtttgag attatgaaac ccaatttgtt ttactttgta ttgacgcaga
      961 ttttagctga atctccaaat tttaaattat tagatgaagt tagaccggtc catgatatta
     1021 ccttaaagga taagaagtat tatcgtttag aattaccctt ggctaatatt gctgcagaag
     1081 gttttagact gcatgacgcg catattagtc tttatgagaa gacagatccc cataatccaa
     1141 atttaggact ttcgcatttt actgcaatct tccatgatca aaatggtcaa acctatcgaa
     1201 tgcatttgtt tttgaatcgt ttcgatgctc tggcatgccc agctacctgg gagttattgg
     1261 atgatgatga gcaatatgtc aaagtttctc caccagaaaa tctggaatat ttcattcatt
     1321 ctgtatggca gctaggcctg ccttgtttgc aattgttgcg aaaaaagcaa aaggaaatag
     1381 aagctaaatt gcttgctgat taccacagat taaacgagga aacgactgca ctaagtaagg
     1441 atttagataa aaacagagcg gtctatttgg aaaagcttga tgaattaatt aagacgacta
     1501 aatcattaag ccaaatttct gaaagtaatc attggttgag agaagcaatt tacctgggga
     1561 aaacatacaa gtacgtatcc tctatgccgg aatcaattct tgagcctgtt aaaaaaagtg
     1621 atgaagagca taaaaaagat gacaaagcag agattgttac cttgtctcca aagcaagggg
     1681 ctaaggagcc caaaatcagc agaggaaata tcgggcttgc tcaatcgacc ttattttcca
     1741 aatcagccaa agcgaaacgc tctgtggatg tgaaagtgga gcgcgatatt agcaagataa
     1801 aaaaattgtt tgctcaacta ttaaagacgg aagataaaaa aattaaagca gttcttttaa
     1861 ttgatttgca ttggaaaatc aaagacatta atttggaaac ggaagagtta ctgacaaaag
     1921 agcaaacaca agccctaaat gaaatcgaag gccatgcaaa taaagaagcc aagtcgctcc
     1981 tggaaagagc tttgtttgct ggagagtttg aatacgctca aaccctgtcg ccttattatc
     2041 ctttgattaa taatgatcta atggttcttg cgcttactca gagaaaagct gatcttttaa
     2101 gctttttagt cactaaagta ggtttgccta tcaatagtta tccaattaaa acaaaagtga
     2161 aaacgtattc caatgccgtt gagtattgct tctcagaaca tagtgaatcg ttggtagact
     2221 gctttagcgt gttaataaaa aatggcgcaa gtttgatgca acctgttggt ttatataaat
     2281 tacctttagc tcatcttctt ttgtctgaaa tccccaggca ccctttgcac gcctcgttag
     2341 aacaaaacaa aaatttaact ctcaataaca agcaatttta tgctcattta attaatgcac
     2401 taaaatcatg tttattatct ggtgatatcg aaggtgacca gaaaattgcc ctggaagagt
     2461 ctattgtgcg atacgagcac ttaaagacta atgtaaaaaa ctcatcctca cttctgagtc
     2521 ctcaaaacca ggcattggct gatgaaataa gcgatattac caaaaaatta ttgcctgacg
     2581 ctgtggctga agcaattgag aatgatgagg aaatatcaag agaaaaagca atcgctgata
     2641 aagaatataa agagttgatt cgtaaggtgc aggcatttta tcataaaacc ggaaagaact
     2701 ttgcttttca atccttaatt aattcagcca atcaggaatt gaagaaagaa ttattagaaa
     2761 ttgactttaa tatcgacatt agctttagcg agcttaagga atccatactg gaaaacttaa
     2821 gcaaggaaag attaatgttc agctattgca gtgaattaat agacgttcaa agaacaatca
     2881 tgcaaatatc gcagggagcg agcagaaaga ataaaaacct gaaaaaactc aatgcgcgac
     2941 atgctgagct tatcgaattg ctcacagatt taagccaaaa tacgcctcaa tctatgataa
     3001 aagaagccaa tgaatttcaa gattctttta gtcagcttca agatttattg aatggcttga
     3061 atcaattgga gggtcctttg ggcatgcttg ctggagtatt aaatcaattc ctggagggct
     3121 caacatcgga atcagaatct tttggtatga agctataaga caaatggcat actagtgttc
     3181 tgacaagcga aatacaatgt agtcttgtgc atcaagcatc atctgtgact ggatttaggt
     3241 gctcaaatga cgcatttttt ttattaaaat caataaaata gatggagcga tcagtaaagt
     3301 gttcttgatc aaaaacacgc caggtaatgt ggcacttttt ctgattcgct tcaatcacat
     3361 aatagctgtt taaattatct ttgctgcgcg gacacagtaa tgagccggca tgcagcgcca
     3421 ggcaggagta gccattattt ttagcaatta aacttaaatg agcatagtgt aaatgacccg
     3481 tgcaaaccaa atgaatggtg ctttgcttta aaaaacttaa aagcgtttgt gcattcgcta
     3541 gtggcttatg taacccttcc atataatcaa aattatgatg aaagaagagc aggtttaatt
     3601 tttcatcatc cggtttaaaa tatcgttcaa ttctatttaa tgtttcatga gatagttttc
     3661 cattctttaa ttgcaatggg ttaacactat taacccccaa aatacgcaca caatcgtttt
     3721 caaaggtggt ggttatatcc gggttgatgt agcgtttgta ctggcgaaaa gggaatataa
     3781 aacgcgctgg aacattataa aaaggaatat catgattacc gggaacagta agcgtttgag
     3841 ctggcagttg atttataaat gagcaaatct cccgatactg atacgagagt ccgcgttgag
     3901 ttaaatctcc tgaaataaga atgatgtctg gtttatcgag ggcggtttcc tgaagaaacg
     3961 catttaaaac tttaggttga tgcatgccaa aatgcaaatc ggaaatatgg ataattttca
     4021 tattttttta gtcagtaatt tcagtgattt aggtaaaatt ttataataaa gagggggcgc
     4081 gatagccagc gtatccccat ctaaagagat ggtaatttta tttttatgaa gaacatcaac
     4141 ctccagaggt aatcttgtct gtattatttc aaaatgctcc ttatccgtta agaaatcaat
     4201 taaaaatttg aataaacgca atttgctgcg tttgtaataa taaatgccca gctgctcatt
     4261 ttgaaaactt tcccgggtaa atttgagagg aaattcatag gtatagcaat tgttgcttac
     4321 catgaaaaag gaagtgcgta ttgatatatc aagttcctta ttttttatta ataaggcata
     4381 agtggggtgg tatgacaaag cccgaataaa actgggaatg taagtaagcc atttattata
     4441 aaaatttgta taataatcac ggttttttgc cagccttgag tagaagccga tagatgagtt
     4501 gttaataaag atatggccat ttacttctgc tacgtcaatt gtggtcgttg ttttttccac
     4561 gattgctgct attaattcct cagtagttga aggtaaattt aactctttgg aaaaatggtt
     4621 catagttcct aaaggaagca caccaagaat aacagacgta ttgctgcacc attgagcagc
     4681 actcctaaca gtgccgtctc cacccccgat taatagtaaa gggtattttg agcaacaatg
     4741 ctgaatattc ttttccaatt gttgagggtc tgctttatat aattgataat caatgtcatg
     4801 cgctctgagt tcagataaat agtcttccat tcggtgtgca tttttagctc gattattgat
     4861 aataacccca atgtctgtca tgacgctccc caatgtcctg cttacttaaa gtttaggtta
     4921 cattcaaaaa ttatagtaag gcatcataag cgcctgtcag ttaatttacc tttactaact
     4981 tgtgtatgtc tatgagacgc attttgaaac ttcaagtggt taaataaaat acagattcca
     5041 tgcttatttt gcttagaaaa tatattatta gtgttagtta tggtattcat aggtaaactt
     5101 gttttagggt actatcagtt tttcttggtt ttgtttaagg tttgcacttt catcaaaacc
     5161 gggagctctc actttttcaa gcaatggtgt cagattaggt ttgaactact acagttaat
//
LOCUS       DBNCWK010000168         5085 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.169, whole genome shotgun sequence.
ACCESSION   DBNCWK010000168 DBNCWK010000000
VERSION     DBNCWK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.169"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            212..1276
                     /gene="pilM"
                     /locus_tag="AC06IO_002650"
     CDS             212..1276
                     /gene="pilM"
                     /locus_tag="AC06IO_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIO9875817.1"
                     /translation="MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEI
                     PASALEGNMIKDVDVVANCIKKLVERLKFTSKNVALAVPDSAVISKVVQINEGLTDLE
                     MEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
                     AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFS
                     REEKFGGMQLVEAIAEHYHMNLEQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTL
                     QFFYSTSQHGFVDHILLAGGMARQPGLASLIQEQLGVTTTVANPLSHMSFGKKVNLDV
                     INSDAPTLMVACGLALRHVE"
     gene            1280..1828
                     /locus_tag="AC06IO_002651"
     CDS             1280..1828
                     /locus_tag="AC06IO_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIO9875818.1"
                     /translation="MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVS
                     HLISNQTNRNQILQKEITALDEQIKEIKALKQIREGLISRMSIVQNLQSTRTLMVHLF
                     DELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
                     NVEKKQSPNNEFKLSFVLKPKY"
     gene            1842..2438
                     /locus_tag="AC06IO_002652"
     CDS             1842..2438
                     /locus_tag="AC06IO_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HIO9875819.1"
                     /translation="MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKP
                     NFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQLQLMNERFGTMLKQLPAKNEMPG
                     LLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
                     IVTLHEFSVEGVSGDGKTVSQDELVMAITAKIYRYRAQ"
     gene            2435..3019
                     /locus_tag="AC06IO_002653"
     CDS             2435..3019
                     /locus_tag="AC06IO_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HIO9875820.1"
                     /translation="MIIKNNQTLILGFFGNSLCTLLTSENFRKSLLTALLVLLLSACS
                     GDNSDLIKYINEVKSRPGRPIEPIPKFAPLPIFRFPENDDRRNPFKPIDQKKRNDIYA
                     PDKKRPKQPLESFPLDALKFVGTLKKDNEIWALIKQPDGQISRVRVGDYMGQNYGRII
                     LIKNDLIKLEETVQKSGTWEKQSTTINLDTGKQE"
     gene            3023..>5085
                     /gene="pilQ"
                     /locus_tag="AC06IO_002654"
     CDS             3023..>5085
                     /gene="pilQ"
                     /locus_tag="AC06IO_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIO9875821.1"
                     /translation="MRKIIAILILISASLSLAYSKDNSLISVKVLPLPENRVRIDFQF
                     SNPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKTKTIQLGSLHSYNIVAVGDRVRA
                     ILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
                     EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITM
                     QQEGKKVRMTILSKGDYGHFVYQVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLN
                     FQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQALDIILTTQGLDKRRK
                     GNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSL
                     LSDRGKISVDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQD
                     IGIRWGVSRPTHLSGTLEGANELARGTAPANVVPLERRLNLDLAAAPLTGATPASVGI
                     ALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQEATSSGA
                     TAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVN
                     NGQTIVLGGIYKQDKNKVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITN
                     ALSITT"
BASE COUNT         1695 a          835 c         1102 g         1453 t
ORIGIN      
        1 catgaaattt gcttgggcat cgacagaaga cagaaatgga aatgcatgga tcaattgtca
       61 gattgattat aaacgcttaa cccaaagtaa taagtaaagt gattccaatt tatagtggct
      121 tacgctaaca ttaattattc aatagttagg aaaagatcta aaaataggag tgcttttggg
      181 ctgcaaggaa atatacaggg agattatata agtgctcaaa ttgcttaaac ctaagcatcg
      241 ttcaattctt ggcattgaca tcagttcttc cgcagtgaaa attctggaaa tatcaggttc
      301 cggagaggac atttgtgttg aaggatatgg tcgtgaagaa atacctgcct ctgccctaga
      361 aggaaatatg atcaaggatg tcgatgtagt agcgaattgt atcaaaaaat tagtagaaag
      421 attgaagttt acatctaaaa atgtggcatt ggctgtacct gattcggctg ttatcagtaa
      481 agtagtgcaa attaatgaag ggttaactga ccttgaaatg gaagagcttg tcgttattga
      541 ggcggataag tacattcctt acccaataga tgaaataaac ctggattttg aagtgttagg
      601 ccattcagcc aagagctcat cactgcttga tgttttaatc gtggcatcaa gggcagaaaa
      661 cgttaatacc cgtgttgaag cagcccatag agcagggctg gaacccaaag ttgttgacgt
      721 tgaatcttat gcagtagagc gttcggtaca acaaattgcg caagaattac cagcaagtgg
      781 gcaagacaaa attgttgcaa tagtagatat tggagccaat tatactcact tatttgtttt
      841 gcatggtatg cgactaatct tttcaagaga agaaaaattt ggaggtatgc aacttgttga
      901 agctattgca gaacactatc atatgaattt ggagcaggct attgccctaa aaaatcaggg
      961 taaactaccg gaggactacg aatctgctgt attagagcct tttaaggaca tgattctttt
     1021 gcagatcaaa cgtactttgc agttttttta ttcaaccagc caacatggat ttgtagacca
     1081 tattttattg gcgggaggaa tggcaagaca accaggattg gcatcgctta ttcaagaaca
     1141 gttaggagtg actactacag tggcaaatcc actatcccat atgtcttttg gtaaaaaagt
     1201 gaatttggat gtgattaaca gtgatgcgcc tacactgatg gtggcttgtg ggttggcttt
     1261 gaggcatgtc gagtaaaaaa tgactgaaat taatttattg ccatggcgtg aacaaaaacg
     1321 tgagcaggaa aagaaattat ttacaaccat gctattgata gggattatca tcgctgcagc
     1381 aattgttttt ttaatgaact cttatgtctc tcatttaata agtaatcaaa cgaaccgaaa
     1441 tcagatttta caaaaagaaa ttacagcttt agatgaacag atcaaggaga ttaaagcatt
     1501 aaaacaaatc agggaaggat taatttcaag aatgtcgatt gtgcagaatc tgcagtcaac
     1561 aagaacttta atggttcact tatttgatga gttaataaaa attactcctc caggagttta
     1621 cataaccaag atgcaaagag aacacgatgt ggtaacactt tgggggtatt ccgaatcgaa
     1681 tacaagtatt tctattttga tgagaaatat agaggccaat gtctggatac agaatcctgt
     1741 tctaactgaa attaaaaaaa atgttgaaaa aaaacagtct ccaaacaatg aatttaaatt
     1801 aagttttgtg cttaaaccca aatattaggc tgataaaaat catggacacg gtcaatttaa
     1861 atgaattaac tctagagaat gttgggcagt ggccaatgcc ggttaaaatc ggagtaattt
     1921 taggcatcag tatactgata attggattag gatattggct tattgttaag cccaattttg
     1981 agcaattgga aatacaggaa gcccaggaaa ttgctttgaa aacagagttt gaaaaaaagc
     2041 aacatcaggc agcaaatcta caagcctatc gtaatcagct acagttaatg aatgaacgct
     2101 ttgggactat gctaaaacaa cttccggcga aaaatgaaat gcctgggctt ttggaggaaa
     2161 tatctaaaac ggggatcgca tcgggtttga aatttgaatt gtttgcacca caacctgagg
     2221 tagtgcatga tttttatgtg gagttaccta taaaaattac gattgtcgga tcctatatgc
     2281 aattggcaag atttttaagt cgtgtagctg aaatgaacag aatagtgact ttgcatgaat
     2341 ttagtgttga gggggtgtcc ggggatggta aaacggtctc tcaggatgaa ttggtgatgg
     2401 ctatcactgc gaaaatatac agatatcggg cacaatgata attaaaaata accagactct
     2461 tatattaggc tttttcggaa attctctgtg cactttactc acctcagaga atttccgaaa
     2521 aagtctattg acggcgttat tggttctatt acttagtgcc tgctctggtg ataacagtga
     2581 ccttattaag tacatcaacg aagtgaaatc aagaccaggg agacctatcg agcctattcc
     2641 caaattcgcc cctttgccaa tttttaggtt tccagaaaac gacgataggc gaaatccatt
     2701 caagcctatt gatcaaaaaa agagaaatga tatttatgcc ccagataaaa aaaggcctaa
     2761 gcagcctttg gaatcttttc ctctggatgc tttaaaattt gtaggtactt taaaaaaaga
     2821 caatgaaatc tgggctctga ttaagcaacc tgacggccag attagccgtg ttagggttgg
     2881 agattatatg ggacaaaatt atggacgtat tattttgatt aaaaatgatc tcattaaatt
     2941 ggaagaaact gtacagaaat caggaacgtg ggaaaaacag tccaccacaa ttaatttgga
     3001 tactggcaag caggagtgag gcgtgagaaa aattatcgcg attttaatat taattagtgc
     3061 cagtttgagt ctggcatatt ctaaagataa ttcgctcatt tcagtaaaag tattgccatt
     3121 gccagaaaac agagtacgta ttgattttca attttccaac cccttaaagc agttgcccgc
     3181 aagctttatt actcaaaagc cgcctcgttt ggtgttagat tttatcagca ctgatatgca
     3241 aattccttcg gatcagaaaa ctaaaactat tcaattaggc tcattacaca gttataacat
     3301 tgttgctgtt ggtgatagag taagagccat actggaactg gatcgttcgg tgtcctattc
     3361 tggcagtacg gcgggctccg tttatagcct tgtcctgaat ggtaaaagta atgatttatt
     3421 tgaaaatagc aaggaggtat tcataacgaa ccaggttgtg aacgccaagc atgaaattaa
     3481 aagaattgat tttcgtggca tagaaaagca gggaggtaga gttttaattg atgtttctga
     3541 tacgggtatt ccaattgatg taactcaagt tgggaaagaa atagtcgtta atttcctgaa
     3601 taccaagatc cctttgaatt taatgaaacg gtatgatgtt tctgattttc acagccctgt
     3661 gcagattatt acgatgcaac aggaaggcaa aaaggttcgc atgaccatcc tcagcaaagg
     3721 ggattatggg cattttgttt atcaggtcaa taaacaattt atggtcgatg tctttccttt
     3781 aactgcggaa gaaattcgtc aggcgaaatt gaagaaacag gtatttaccg gtaaacggat
     3841 ttctttgaat ttccagaata ttagtattcg agctgtgctt caactactgg cagattttac
     3901 cggaatcaat atggttgtca gtgacaaagt gcaaggagat attacgctta gattaaatga
     3961 tatcccctgg gatcaggctc tggatatcat tttaaccaca caagggctag ataagcgcag
     4021 aaagggcaac gtcatgttga tagataccaa agcctccatg gataaaatgg aggaagaaca
     4081 actcaaaagt caacagacaa ttgaaaaact tgagcctatc cgctctgatt taatacagat
     4141 taattatgca aaggcagcag atattgctgt tttgatcaag gataagcaaa actccctatt
     4201 atcagacaga ggaaaaataa gtgttgatgc cagaacgaat acaatctgga ttcaggatac
     4261 tggcaccaaa attgaggaag taagggaact gataaagcaa ttggatatcc ctgttaagca
     4321 agtgcttatt gaggcaagaa ttgttgaagt aactaaagat tttagccagg atattggaat
     4381 acgctggggg gtatcaagac caactcactt aagtggtaca ctggaaggtg ctaatgagtt
     4441 agcgagaggc acagcaccag ccaatgttgt ccccttggaa agaaggttaa atcttgattt
     4501 ggcagctgct cctttaactg gagctacacc ggcctcggta ggtattgcgc tagctaaact
     4561 taatgataac attctgcttg atctggagtt gtctgctttg gaaagcgaag ggcgtgctga
     4621 attaatatcc agtcctcgcc tgattactac gaatcaacag gcagcagtca tagagtctgg
     4681 tgaggagatt ccctatcagg aggcaacatc aagtggagcg actgctgttg catttaaaaa
     4741 agcagtattg agtttgaaag ttacgccgca aattacgcct gatggtaaaa ttttgatgga
     4801 tttacagata aatcaggata ctccttcccc acaaacattc cttggtgttc cagcaataat
     4861 aacaaaggag attcagacga atgttctggt taataatgga caaaccatag tacttggagg
     4921 tatttataag caagataaga acaaggtaat caatagaatt cctttctttg gtgagttgcc
     4981 tgttgtcggt attttattta gtaatagaca aatcacattg aaaaatgaag aattacttat
     5041 ttttattaca ccaagaataa taacgaatgc cttatcgatt acaac
//
LOCUS       DBNCWK010000169         5082 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.170, whole genome shotgun sequence.
ACCESSION   DBNCWK010000169 DBNCWK010000000
VERSION     DBNCWK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.170"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(515..1747)
                     /locus_tag="AC06IO_002655"
     CDS             complement(515..1747)
                     /locus_tag="AC06IO_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9875822.1"
                     /translation="MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTAL
                     IATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTMTLMVMTFSSSFIGLLPTYQEAG
                     ILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNMRHKGLAVSFVTLGSICGLLLAII
                     VSKITLIFFSSQSPDFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPIL
                     NAWRYRKKAIAAIFLLAIYCNSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSM
                     GLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLHITVACSVLAVIISFF
                     IGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMV
                     MALLTLPALWLIQEKGTN"
     gene            complement(1788..3092)
                     /gene="sdbC"
                     /locus_tag="AC06IO_002656"
     CDS             complement(1788..3092)
                     /gene="sdbC"
                     /locus_tag="AC06IO_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbC"
                     /protein_id="HIO9875823.1"
                     /translation="MPNSSIQIWLQRQIALRTFPPNREDWYVKNGFGNSEMSLEERFA
                     CFVEAIRQEDSPYHEQLSGIDFHRFEIEVTLANQKTCMTEVIGFQNNSNQSSSLLNEK
                     VKAVGKGKHVIYFTGIGTQYQDCLIDIAKAVQITGAHYYAFEYPGMRKLGGEVLEVND
                     MVNTGIALANTLLEKGISIDDILFQGDSFGAAVAKKVSDQFKLQSNVTVRCILNNTFS
                     TFQAAVEGNLGAFSTLRYTVRPLLRYTGWDIRPGDNYGLDTPYQIHINHTGDLTLGAG
                     HATLAESVESNSQLEHFVDPCPEEYRIKRDIYKQWHWASLSEQGIRFLETKYGRNSDG
                     QLDTHLADLYLLKYDTGLGVYESFICKYLEDSNDYIANHPQKLSLEELPSPLESETVS
                     LGQLIVSVLPSRPSIAGFFGRPKPVKDEVDSENQAQKSIELK"
     gene            3355..4239
                     /locus_tag="AC06IO_002657"
     CDS             3355..4239
                     /locus_tag="AC06IO_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875824.1"
                     /translation="MFYELILSRSSNLIQEFSYIPHGVTSLDLSLNELGSISNAELIQ
                     AFKYIPDSVTSLDLSNNHLCDKSGAELAQLLAAISVNVTSLNLSSNYLDRKSGAELAQ
                     AFAAIPASVTSLNLHCNYLGNNRGVELAQAFAATPKNVTSLDLSMNYFDLESSADLSQ
                     IFTSIPPHVVSLNLSFNSLHEVPFEKLVLLKDSLKHVQTVYLSFYSVKEMSKEQRRAL
                     GSAFPNAQKIILVDDYDNEIQPSITISNLIGELSGKADAPSLLNQCILFAQRNQIDYM
                     KRNIPGELQESIRAFNSR"
     gene            complement(4360..4824)
                     /locus_tag="AC06IO_002658"
     CDS             complement(4360..4824)
                     /locus_tag="AC06IO_002658"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIO9875825.1"
                     /translation="MKHRRSETQDIDDAIIKTLNKLLEIELAGVVRYTHYSFMVFGFN
                     RIPICKWMRDQAQESLNHAHLIGELITHFSYHPTLKIGKLLETHEHNISNILEESLEH
                     ERQSLDLYYQLLQYSENKSVLVEEFARQMIQEEEMHLGEISKMLNSPTTFKD"
BASE COUNT         1624 a          984 c          914 g         1560 t
ORIGIN      
        1 ctctcaacta ttctctgata cttggcaaaa aatttctgaa tactttgatc tcaaaattaa
       61 ttacccatga gaaaacactg aagtgccttt aacttctttt atctggtgga tttgaaattt
      121 ttaaaaacag agggctatct gatatagcga gattttttcg caccttagag attgatttcg
      181 tatatcaggg ttgataccac tttttttcag catttgttgg tccgcagtaa cttgctttta
      241 ccgagctgaa aaactgattt ttgttatgta ttaaattgca aatccttttc aagttttgca
      301 tttcttccgg agaaataaaa actggtgcag tatctgacaa accaattggc ttgagaatcc
      361 acaattttgg aaactatttt tttcataaat agaaattttt tataaggtga gaccaacttc
      421 cgtatgtccg cagtcaggat aaatatattt tacacaacca aactgggcac atttttgtgt
      481 tagatcactg tttttcgtac cataaactaa taaattaatt cgtgcctttt tcttgaatca
      541 accatagggc aggaagggtc aacaaagcca tcaccatcaa ataaatagaa aatgcaaaat
      601 ggatgaaagc ataacttaat agcaaaataa tgagtggtgc tgtgcctcca aatagggaat
      661 tagacaagtt aaaaaataaa gtaaagccac tggcacgctc ttcctgcggt aattgttcaa
      721 ggctggcggt aaatgcgctt ccaataaaaa aagagataat gacagccaaa acactgcagg
      781 ctacagtaat gtgtaataaa gtacctgtat tgatcaaata atttattacc aaaacggtac
      841 ataaagttgc aattaaactg actgccaaag gaaagcgcct gcctaaaata tcagaggtga
      901 aacctgataa taagagaccc atgctataac aaatcatccc tatgataatg acatccatcg
      961 ctgtagttaa tggcaactcc tgctgttgca gatatgaagg catataaaca aaaaatgtat
     1021 aaaagctact gttgcaataa attgccaaaa gaaaaatagc agcaattgct tttttccgat
     1081 aacgccaggc attcagtatg ggatgagatt gtctttgaac aattactttt gcaaaatcgg
     1141 ttaattctct ggtttgactt cgcaaataaa gtccgataaa acctacgaat attcctatta
     1201 aaaatgggaa gcgccagaga aaatcgggag actgactgga aaaaaatatc agggttattt
     1261 tgcttactat tattgcaagc aacaatccac aaatagaacc taaagtgacg aaactgactg
     1321 ctaaaccttt atgacgcatg ttcacatttt cataaatgta aatactggct agtgtcattt
     1381 cccctgctac agaaacccct tgagttaatc gacatagcgt gagtaatatc ggagcgagaa
     1441 tgcccgcctc ttgataagta ggtaataaac caataaaact tgatgaaaaa gtcatgacca
     1501 taagagtcat agtcaatgtt tttttgcggc caattttgtc acccagattc cctaaccaat
     1561 aagcagagaa tggccgaaca agaaaacctg cggcaaaaat accaaatgtt gcgatgagag
     1621 cagtgtgttt atcagtggca ggaaaaaaaa gaggcgccaa aatgggcgcc ataaacccat
     1681 aaatggcata gtcgtacatt tcaaacaagg tgccaaaaga ggctgccgca ataatttttt
     1741 tatccatgtt caagctgttg cttttgtttc acaggtagta atacagctta ctttaactct
     1801 attgatttct gagcttgatt ctctgaatcc acttcatcct tgactggttt aggacgacca
     1861 aaaaaaccgg ctatactagg ccttgatggt aacacagaga caatcaattg tcccaatgaa
     1921 actgtttcgg actcaagagg acttggtaat tcctcaagcg agagtttttg cgggtgattg
     1981 gcgatatagt cattactgtc ttcgagatat ttacaaataa atgattcata aacccccaga
     2041 cctgtatcat actttagcaa atacaaatcg gccagatgcg tgtccaattg accgtcactg
     2101 tttctgccat atttcgtctc gagaaatctt attccttgtt cgcttaatga ggcccaatgc
     2161 cactgtttat agatatctct tttaatacga tactcttcag gacagggatc aacgaaatgt
     2221 tcaagttgag aattggattc aacgctttct gcaagcgttg catgccctgc ccctaaggta
     2281 agatcacctg tatgattgat atgaatttgg taaggggtat ccaggccata gttatcccca
     2341 gggcgaatat cccaaccagt atagcgaagc agagggcgaa ctgtataacg caaagtagag
     2401 aatgcaccca gatttccttc gacggcagct tgaaaagtgg aaaaggtatt attcagaatg
     2461 catcttactg ttacattgga ttgcagtttg aattgatcac tgactttttt tgccaccgca
     2521 gcaccaaagg agtcgccttg aaaaaggata tcatcaattg atatgccttt ttcaagtaag
     2581 gtattggcca gcgctattcc agtgttaacc atatcattga cttctaacac ttcaccgccc
     2641 agttttctca tacccgggta ttcaaaagcg taataatgcg ctcccgttat ttgaacagct
     2701 tttgctatat caatcaggca atcttgatat tgtgttccta ttccagtgaa ataaatcaca
     2761 tgttttccct ttccaacagc tttgaccttc tcatttagta aggagcttga ttggttggaa
     2821 ttattctgaa accctatcac ctcggtcata caggtttttt ggttggctaa cgtcacctcg
     2881 atctcaaagc gatgaaagtc tatacctgat aattgttcat ggtagggaga atcctcttga
     2941 cgtattgcct ctacaaaaca ggcaaatcgt tcttcaagtg acatctctga attaccgaaa
     3001 ccatttttga cataccagtc ctctctattc ggaggaaaag ttcttaaagc tatttgtctt
     3061 tgcaaccaga tttgtatact tgaattcggc ataacactcc cctttttttt gatcaataag
     3121 tgtgcataca atttgctaat gagtcaacat ttctttgcgc atctttatta gagataattc
     3181 ttatgttcag caccgatttg aacccaattg caacaaagca aactcacttt aatcctgcgc
     3241 ttataagaaa cttgtataaa caatatacaa ataattaatt ttaattatga ccagtaggtt
     3301 gatattatat tcatattgaa taaaatatgt cccgttattt aggagatttt cataatgttt
     3361 tacgaattaa tattaagcag aagcagcaac ttgatacaag agtttagcta tatccctcat
     3421 ggtgttactt cgcttgattt gagtttgaat gaattaggca gtataagcaa tgctgaatta
     3481 attcaggcat ttaagtatat acctgacagc gttacttcgt tggatttgtc taacaatcac
     3541 ttatgcgaca aaagcggtgc agaattggca caactcctgg ctgccatctc cgtcaatgta
     3601 acttctctaa atttaagctc taattatcta gacaggaaaa gtggcgctga gttagcacaa
     3661 gcctttgctg ctatcccagc cagtgtaact tcgcttaatt tgcattgtaa ttacctgggc
     3721 aataaccgtg gggtggagtt agcacaggcc tttgcggcca cccccaaaaa tgtgacctca
     3781 ctggatttaa gtatgaatta tttcgatctg gaaagtagtg ctgacttatc acaaattttt
     3841 accagtatcc ctcctcatgt ggtgtcactt aatttgagct ttaactcctt acatgaagta
     3901 ccctttgaaa agctagtact ccttaaggat tcgctaaagc atgtgcaaac tgtttatttg
     3961 agcttttata gtgtcaaaga aatgtccaaa gaacaacgaa gagcactggg ctctgcattt
     4021 cccaatgcgc aaaaaatcat tttggtggat gattatgaca atgaaattca accatcaata
     4081 acaatttcta acctcatcgg ggaactttct ggaaaggcag atgctccatc cttgttaaat
     4141 caatgtattc tttttgcaca aaggaatcaa atagactata tgaaaaggaa tatccctggt
     4201 gaattacaag aaagtattcg agctttcaac tctcgctgat tgataatctt gaacaaccgt
     4261 cgttcgctct tgtcaatgaa ttaagaaaca ccaaaaactt gtttgactct tccgagtcaa
     4321 acaagaaatg gaataatcga caaagaaaaa gaactctcgt taatctttga aagttgttgg
     4381 ggaattaagc attttggata tttcgccaag atgcatttct tcttcttgaa tcatttgtct
     4441 tgcaaattct tcaactaaca ccgacttatt ttctgaatat tgtaatagtt gataatacaa
     4501 atccagactt tgcctttcat gctcaagcga ctcttctaaa atattgctga tattatgctc
     4561 atgagtctct aataacttac cgattttaag tgtaggatga taggaaaaat gggtaataag
     4621 ctcaccgatt agatgagcgt ggttcaaact ttcttgagcc tgatcgcgca tccatttgca
     4681 gatcgggatg cgattaaatc caaagaccat aaaggagtaa tgagtatagc gcacaacacc
     4741 cgccagctcg atctccaata atttatttag agtttttatg atggcatcat caatgtcttg
     4801 agtttcagat cttctatgtt tcataggatt acctgtcatt ttacttatct aagtatagaa
     4861 taatttcaag aaaatctcga agcgttatca cttattgaga attgcctctc atatctaaca
     4921 taagccctga tacaaagatg acatctggca gtcttggata cataaaaaac tggctacaca
     4981 aaataattat tcaattttgc caatatattg ccacttctat cattcaaggc ggcttgatgg
     5041 aagaaaatta tgttcttttt atatactact taaggattaa ta
//
LOCUS       DBNCWK010000170         5049 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.171, whole genome shotgun sequence.
ACCESSION   DBNCWK010000170 DBNCWK010000000
VERSION     DBNCWK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.171"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            138..1400
                     /gene="rho"
                     /locus_tag="AC06IO_002659"
     CDS             138..1400
                     /gene="rho"
                     /locus_tag="AC06IO_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIO9875826.1"
                     /translation="MNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHAL
                     KGEDIHGDGVLEVLTDGFGFLRSADGSYLAGPDDIYVSPSQIRRFGLRSGDTISGKIR
                     PPKDSERYFALLKVDQINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDLTAR
                     VVDLCAPFGRGQRGLIVSPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTE
                     MQRSVKGEVVASTFDEPANRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRLYGAARNIEEGGSLTIIATALVDTGSKMDEVIYEE
                     FKGTGNMEIHLSRNIAERRVFPAININRSGTRREDLLLSPEELQRTWILRKILQSMDE
                     CDAIEFLLERMKNHKTNAEFFDAMKRQE"
     gene            1545..3011
                     /gene="ubiD"
                     /locus_tag="AC06IO_002660"
     CDS             1545..3011
                     /gene="ubiD"
                     /locus_tag="AC06IO_002660"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HIO9875827.1"
                     /translation="MKYSDLRDFIAQLESRELLKRIDYPVSPHLEMTVVSDKVLRSGG
                     PALLFTNTPNYEMPVLTNLFGTVERVALGMGEESIVALREIGKLLAALKEPDPPKGFK
                     DAFSKLPLLKQALNMAPKYVSGAECQTHVWEKDEVDLTLLPIQTCWPGDVAPLITWGL
                     VTTRGPHQSRENMGIYRQQLLSKNKLIMRWLSHRGGALDYQAWQQEYPKERFPVAVTL
                     GADPATILAAVTPVPDTLSEYAFAGLLRGQRTRLTRCIGNDLHVPASAEIVLEGYLEP
                     GNEAPEGPYGDHTGYYNEVQSFPVFTVERITHRDKPIYHSTYTGRPPDEPAILGVALN
                     EVFIPLLQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYPGHAKRIMMAVWSFLRQFMYT
                     KFVIVCDDDVDARNWQDVIWAMTTRMDPSRDTVMVENTPIDYLDFASPVSGLGSKMGM
                     DATSKWPGETQREWGKPITMDEDVLNRVNGYWSLLGLK"
     gene            3011..3724
                     /locus_tag="AC06IO_002661"
     CDS             3011..3724
                     /locus_tag="AC06IO_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HIO9875828.1"
                     /translation="MTDKIIQAQVEDISPLTDSIVRLVLMPDEYVDYQAGQYLQILFA
                     NDAFSYSIANAPLGSHRYELHIRHSQDNDYHQSLLAHIKQYGSLRLRLPLGECSIDQL
                     HPQKPILFIAGGTGFAPIKAMIEQLLADSDSRPFELFWGARSQSDLYMDDKVRSWRSH
                     ASHFQYFSLLSDKSEDTLVSRVLARHANILRDWQIVISGPFDMVYSTRDTLVGYGISP
                     TQMFSDAFSFESKTDDVKN"
     gene            3743..3937
                     /locus_tag="AC06IO_002662"
     CDS             3743..3937
                     /locus_tag="AC06IO_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875829.1"
                     /translation="METLYQILGLIGAGLIIFILYRFIKGSPGQFSKENMSKSFMTMG
                     VLGLILIGFIALLVLMLRNT"
     gene            complement(3975..4286)
                     /gene="hspQ"
                     /locus_tag="AC06IO_002663"
     CDS             complement(3975..4286)
                     /gene="hspQ"
                     /locus_tag="AC06IO_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HIO9875830.1"
                     /translation="MNKIAKYNIGDLVIHKRIRYRAIVIDVDPIFQASGKYNPRTTQH
                     EFATRNPWYRLLVDDSSQITYVEECQLLPDTSQEPINNPNIDDYLIKNKGSYQKTIPS
                     H"
     gene            complement(4348..>5049)
                     /gene="tatC"
                     /locus_tag="AC06IO_002664"
     CDS             complement(4348..>5049)
                     /gene="tatC"
                     /locus_tag="AC06IO_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIO9875831.1"
                     /translation="IELRQRAIYTLLWFGALALLFFFISDQLFHLLVRPLLRSLPNQE
                     GLIATQITSSVFTPLKLAVDTALLLTAPFALLQLWRFMRPGLYKNEQEQIRGTIILSL
                     LLFVFGTLFCFYLVLPYMFQFFAQALPEGVRLMPDMAYAIDFITRMLLLFGFSFQVPL
                     ICLVLVKTHFTTVETLIKIRPYVIVGAFVIGMLLTPPDVFSQVALALPLCLLYEAGII
                     LARIYGKSPVPTLIQ"
BASE COUNT         1459 a          991 c         1160 g         1439 t
ORIGIN      
        1 gtgatagcct gtagctatat gtgacatact ttaaaacaag tgagccacat aggcagcgga
       61 ttccttagct gctattccct aacataattt tttcttcgga tattcaattc atttaaacaa
      121 caaatcaagt gagaagtatg aatcttagtg aacttaagca attacctatt gccgatctcg
      181 ttaatatagc tcaagagatg ggtgtggaaa atacctcccg tatgcgcaag caagacatca
      241 ttttcgccat ccttaaagcc catgctttaa aaggggaaga catccatggc gatggtgttt
      301 tggaagtttt gactgatggt tttggtttct tgcgttccgc tgatggctcc tatctggcag
      361 gccctgatga catttacgtc tctcccagtc aaatcagacg tttcggtctt cgttcggggg
      421 atactatttc tggtaaaatc cgccccccca aagacagcga acgctatttt gctttattaa
      481 aagttgatca aattaactac gatacaccgg atagcgcaaa gcgtaagatt ttatttgaaa
      541 acttaactcc cttgtttgct accgagcgtc tggttatgga gcaaggtaat ggcagcacag
      601 aagatctcac agcaagagta gttgatttat gcgctccttt cggccgcgga cagcgtggtt
      661 tgatcgtgtc acctccgaaa gcaggtaaga cattaatgtt gcaaaacatt gctcgctcta
      721 tagaaaaaaa ttatcctgaa tgttatctca ttgttttgct gattgatgaa cgtcctgaag
      781 aagtaacgga aatgcagcgt tctgtcaaag gggaagttgt tgcgagtact tttgacgagc
      841 ccgccaatcg ccatgtccag gttgccgaaa tggtaataga aaaagcaaaa cgtctggttg
      901 agcataaacg tgatgtggtt attctgctcg attcaatcac tcgcctggcc agagcttaca
      961 atacagttgt accagcgtca ggtaaagtgc tgactggtgg tgttgatgcg aatgccctgc
     1021 aaagacccaa gagattgtat ggtgcggcac gtaacataga agagggtggc agtttaacca
     1081 ttattgcaac ggctctggta gatacgggtt ccaaaatgga cgaagtgatt tacgaagagt
     1141 tcaaaggtac aggtaacatg gaaatccact tgagtcgtaa tattgccgag agaagggttt
     1201 tccctgcgat taacattaat cgctctggta ctcgccgtga agatctcctg ttgagccccg
     1261 aagaattaca gcgcacctgg atattgcgta aaatcctgca atccatggat gaatgcgatg
     1321 cgattgaatt cttattggaa cgcatgaaga atcataagac aaatgccgaa ttctttgatg
     1381 caatgaagcg tcaggaataa tttaaatgat aattaacccc gtaattagcg gagcataatt
     1441 cggggttcaa attcgcctta ttttcaggtt tataattcat gctcatagcc tgtattacgc
     1501 tgtgctggta tgggtttaca gtaagcttct ttttgagata attaatgaag tattcagatc
     1561 tgagagattt catagcccaa cttgaatcac gtgaattatt aaaacgtatt gattatcctg
     1621 tatcacctca tcttgagatg accgtagtca gcgataaagt gttgcgctca ggagggccag
     1681 cccttctgtt taccaatacc cccaattacg aaatgcctgt actgaccaat ctttttggta
     1741 cggtagagcg cgtggctttg ggaatgggtg aggaatcaat agtggctttg agggagattg
     1801 gaaaattatt ggctgcttta aaggaacccg atcctcccaa aggcttcaaa gacgctttta
     1861 gcaagttgcc tttattgaaa caagcgctga atatggcacc caaatatgtt agtggagccg
     1921 agtgccagac tcatgtgtgg gaaaaggatg aagtggattt aaccttattg cccatccaaa
     1981 cgtgttggcc cggagatgtt gctcctctaa ttacctgggg tttggttact actcgtggcc
     2041 cacaccagtc cagagaaaac atgggcatct atcgccagca actattaagt aaaaacaaat
     2101 tgatcatgcg ctggttatct caccgcggag gtgctttgga ttaccaggcc tggcaacaag
     2161 aatatcccaa agaacgtttc cctgttgcgg tgactttagg cgctgatcca gccactatac
     2221 tggcagcagt tactcccgtt cctgatactt tgtctgaata cgcttttgcg ggcttgctta
     2281 gaggacaacg aactcggttg actcgatgta ttggcaatga tttgcatgtt ccagccagcg
     2341 cagaaattgt tttggaaggt tatctggagc caggaaatga ggcgcccgaa gggccttatg
     2401 gcgatcacac cggttattat aatgaagtcc aatcttttcc tgtttttacg gtagagcgta
     2461 ttactcatcg cgataaacct atttaccaca gtacttatac cggaagaccg ccagatgagc
     2521 cagccatttt gggggttgcc ttaaatgaag tgttcattcc cttgttgcaa aaacaattcc
     2581 cagagattgt ggatttttat ttgccgccag agggatgctc ttatcgtttg gctgtagtca
     2641 ctataaaaaa gcaatatcca ggacatgcta aacgtattat gatggctgtt tggtctttct
     2701 tgcgccagtt tatgtatacc aagttcgtca ttgtttgtga tgatgatgtg gacgcgcgca
     2761 attggcaaga tgtcatatgg gcaatgacga cacgcatgga tccgtcccgc gatacagtta
     2821 tggtagaaaa tacacccatt gattatctgg atttcgcttc cccagtttca ggattgggtt
     2881 ccaagatggg tatggatgct accagtaaat ggccaggaga aacacaaaga gaatggggta
     2941 aaccaattac gatggatgaa gatgtgctta atagagtaaa tggttattgg tccttattag
     3001 gattaaaata atgacagata aaataattca agctcaggtg gaggacattt cccctttaac
     3061 ggacagcatc gtgcgattgg ttttaatgcc tgatgaatac gttgattatc aagccggaca
     3121 gtatttgcaa atcctgtttg caaatgatgc atttagttat tcaattgcaa atgcgccttt
     3181 aggttctcat cgttatgaat tgcatattcg ccatagccag gataatgatt atcatcaatc
     3241 gttattggct catatcaaac aatatggatc tctgcgctta agattacctt tgggtgaatg
     3301 cagtatcgat caattacatc cccaaaaacc tattttattc attgccggcg gaactggatt
     3361 tgcaccaatt aaagccatga ttgagcaatt actggcagat tcggacagca ggccatttga
     3421 gttattttgg ggagcacgct cacaaagcga tttatacatg gatgataaag taaggagttg
     3481 gcgctcccat gccagccact tccaatattt ttcgctatta tccgataaaa gtgaggatac
     3541 tttggtttct cgggttcttg ccaggcacgc aaacattcta cgggactggc aaattgtcat
     3601 cagtggtcct tttgatatgg tgtacagtac tcgtgatacc ttggttggtt atggcatatc
     3661 gcctacccag atgttttcag atgcctttag ttttgaatcc aaaacagatg atgtaaaaaa
     3721 ctgagtggaa tggagtgaat taatggaaac tttatatcaa attttaggac tcattggcgc
     3781 cggattgatt atttttatat tgtaccgatt tattaaggga agcccggggc aatttagcaa
     3841 ggaaaatatg agcaaaagct ttatgacgat gggggtttta ggcctgattt taataggttt
     3901 tattgccttg ctggtgttga tgttgaggaa tacataacaa gctccatgcc ctgttggcat
     3961 ctccgcctca taagctagtg agacgggata gttttctgat aactgccctt gtttttaatt
     4021 aaataatcat caatattggg gttgttgata ggttcctgac tcgtatcagg aagtaactgg
     4081 cattcttcga cataagttat ttggctgctg tcatcaacta acaggcggta ccatgggttt
     4141 cttgttgcga attcatgctg agttgtccgg ggattgtatt tccctgaagc ctggaatatg
     4201 ggatccacat caataacaat cgcccggtat ctgatgcgtt tatgaataac cagatcaccg
     4261 atattgtatt tggctatttt attcataaaa ggctccattt ttgagcatta tgataaatat
     4321 atcggtaact ccgcttttaa cttgagctta ctgaattaaa gtgggtacgg gggatttccc
     4381 ataaatcctg gctaaaataa tccccgcctc ataaagcaga cacaaaggca aggctaatgc
     4441 aacttgagaa aaaacatcag ggggggtgag tagcatacca ataacaaatg ccccaactat
     4501 cacataaggc ctgattttta taagtgtctc aaccgtagtg aaatgggttt ttaccagtac
     4561 aagacaaatt agtggaacct gaaaagaaaa accaaacaaa agcagcatgc gtgtaatgaa
     4621 atctatggcg taagccatat caggcatgag tcgcacccct tccggaaggg cctgagcaaa
     4681 aaactggaac atataaggga ggacaagata aaagcaaaat agtgtcccaa agacaaagag
     4741 caaaaggctt aaaatgatgg tgcctctgat ttgttcctgc tcgtttttat aaagtcccgg
     4801 acgcataaaa cgccaaagtt gcaataaagc aaacggagcg gtgagcaaca aagcagtatc
     4861 tactgcaagt tttaaagggg tgaaaaccga ggaagtaatc tgtgtggcaa tcaagccctc
     4921 ttgattcggt aaagatcgca ataacggcct taccaggaga tgaaacagtt ggtctgatat
     4981 gaaaaaaaac aatagcgcta aagcgccaaa ccagaggagg gtatagattg ctctttgccg
     5041 taattcaat
//
LOCUS       DBNCWK010000171         5039 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.172, whole genome shotgun sequence.
ACCESSION   DBNCWK010000171 DBNCWK010000000
VERSION     DBNCWK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.172"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            59..1030
                     /locus_tag="AC06IO_002665"
     CDS             59..1030
                     /locus_tag="AC06IO_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIO9875832.1"
                     /translation="MYTERSIFKLGKEKSGAIQIGKRGLEQHLLEADICQPFYIKELL
                     SKQDWSIFEEPNKKKKRKPYPAQSMVGLILYGLMQGLSSLRDLERLARVDLGCILITD
                     GIFPDFSSIGYFIRRHAEVLNEAFFIQLTRSILKVTRSGVRSLAGDGTIIEATSSRYT
                     LMRHEAIEKKREALKAQLAAHPEEQNLGAAAEKIEQASTIMQGRIEKRKEQRKPIDTV
                     TINPTEPEAVVQPTKKGKTAVPAYKPSVLANKARVVVALDVHPSNEIDSLVSMLDMTN
                     RANYQEKVEELLLDGGYESHGVLEETLKRDISVLCSSSDNSKKKHKK"
     gene            1039..1512
                     /locus_tag="AC06IO_002666"
     CDS             1039..1512
                     /locus_tag="AC06IO_002666"
                     /inference="COORDINATES: protein motif:HMM:NF025126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIO9875833.1"
                     /translation="MQKKEFVYHERTDAYTCPQGQQLFPLRRGAASATAPAYTTYATK
                     ACQDCPLKKKCTSSKMGRQIKRYKGDELKEALALVMQQPQAQKRLRQRKAMVEPVFSH
                     IRIRQNFNRFKRRGLHGVKVEFTLQIMAYNISRAIARYFSFLIRLIINHIGNFVF"
     gene            1731..4997
                     /locus_tag="AC06IO_002667"
     CDS             1731..4997
                     /locus_tag="AC06IO_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIO9875834.1"
                     /translation="MLNEAISKMPEAFEAKILLRGEEYFQKGQVLNIRLSDGLLKGRV
                     KGSASQIYDIHMDLKSWPAKPSRCSCAYQYNCKHAAACLFALRDREKINLQSLPSNKL
                     DRKLDVWLKNLRAQEASEIKREEATHHLIYLIDLKLRGYEHKVSIKLALAKLLKRGGY
                     GKKIIFNTLANSKKQHFMGDDNDIVAQLLFKCNVSGWFDNLLIRNTELLDKIIATGRA
                     FFIENEDIPIKKGETLTGECQWILSWNGNQILKLRHDNQNIEPLLLDESWYFDIKNAV
                     IGHLNSPYPIKQLRHLLEAPPIPLDQAELLAKKMSKNCPEFPMPNVFNQKETKQLKPI
                     PVLILDAVTEQEEHRWIDDGEENNSLYIAKIFFDYEGLLVPFAEECEKVVSLKGDVLV
                     EYQRDQEFEDLKIQELRNSIDLRPFHQWEKYHWNLPDHSFFILKDVQIVSDLESLYQQ
                     SLPYLKDHGWRIEFASPFYQEIVSADEVEWYSDIQESTTDFFSYQLGILVEGKPVSIV
                     PLVADLIQRYKGNDLDNLPDEQLVKLPLLDGRLLQLPMGRIKPLVRLLLQFGLRQIDQ
                     NQQLQINKYQLVLMQEVELAIAATKSRWQGAESLREELRKLVRLNDLPEIKVPSGLQA
                     HLRDYQHYGLNWLQFLRVSRFNGVLADDMGLGKTVQTLAHLQYEKEQSRLHKASLIIA
                     PTSLVGNWFAEAKRFTPEIKVLIYHGSDRHQDNFDDYDLIISTYGLIHRDKEKFVGYP
                     FYYLILDEAQFIKNARTKTTQIIQQLKASHRLCLTGTPLENHLGELWSLFHFLMPGLL
                     GDAKQFRLWFRTPIEKYADMGRRDLLAKRVQPFLLRRTKNQVANELPPKTEITRTIEL
                     VGAQRDLYEAIRMSMEKKVRDAIARQGLGKSHILLLDALLKLRQVCCDPRLLSMSEAE
                     IAHGTSCKLDALMELLDNLVEEGRRVLVFSQFTSMLKLIEELLIARQYDYLKLTGQTQ
                     NRQALVEQFQQGDTPIFLISLKAGGTGLNLTRADTVIHYDPWWNPSVEDQATDRTHRI
                     GQESPVFVYKLITSGTVEEAILGMQEKKRQLVEGILSTDASRSMVLSEEDLNQFFMPL
                     "
BASE COUNT         1626 a          866 c         1097 g         1450 t
ORIGIN      
        1 tgctcttgat atagattgaa acctgattaa gatagtgatt ttttgaaaag gatttaaggt
       61 gtatacggag cgttctattt ttaaactagg caaagaaaaa tcaggggcta ttcaaattgg
      121 gaaacgaggg cttgaacaac atttgctaga agcagatata tgtcagcctt tttatataaa
      181 agaattgtta tctaaacagg attggagcat ttttgaagag ccgaataaaa agaaaaaaag
      241 gaagccctat cctgctcaat cgatggtagg attgatttta tatggtctta tgcaaggttt
      301 atcttcctta cgagatttag agcgtttagc gcgagtagac ttgggctgca ttctgataac
      361 ggatggaatt ttccctgatt tttcaagtat tggttatttt attcgtcgcc acgcagaggt
      421 tttaaacgaa gcctttttta tccaattaac ccgctctatt ttgaaagtga ctcgttcagg
      481 ggtccgttca ttagcggggg atggaacgat tattgaggcg acatcctccc gttatacatt
      541 aatgagacac gaggctattg agaagaaacg agaggcatta aaagcccaat tggctgctca
      601 tcctgaagaa caaaacttag gcgcggctgc ggaaaaaata gaacaggcct cgactatcat
      661 gcaaggtcga attgagaaaa ggaaagaaca gcgtaagcct attgatacgg taaccattaa
      721 tccaacagag ccagaggcgg tggtgcaacc gaccaaaaaa ggtaagaccg ctgttcctgc
      781 ttataaacct tccgtgctgg ccaacaaggc cagggtggtt gtggctttgg atgtccatcc
      841 atcgaatgaa atcgattccc ttgtctcgat gctggacatg actaatcgag ctaattacca
      901 agaaaaggtt gaagagttac tgttagatgg aggctatgaa tctcatggtg ttcttgaaga
      961 aaccctgaag cgtgatatca gtgtattatg ctcttcgagt gataacagta aaaaaaaaca
     1021 caaaaaatag ctaaaaaaat tcaaaaaaaa gagtttgttt accatgagag gacagatgct
     1081 tacacctgtc ctcaagggca acaactcttc cctttaaggc gaggagctgc cagtgcaaca
     1141 gctcctgctt atacaaccta tgcaactaaa gcatgccagg attgtccttt aaagaaaaaa
     1201 tgcacatcaa gtaagatggg ccgtcaaatc aaacgataca agggcgatga gctcaaagaa
     1261 gcgctcgcct tggttatgca gcagcctcaa gcacaaaaac gactaagaca gcgcaaagcc
     1321 atggtagaac cagtcttcag tcacattaga attcgtcaaa actttaatcg ctttaagcgg
     1381 agagggcttc atggcgtaaa agtcgaattt acactgcaaa tcatggctta taacatcagt
     1441 cgagctatcg ctcgatattt ttctttcctt attcgattga taattaacca tattggtaat
     1501 tttgtattct aatgccataa gtcatttcaa atacaacaat aaatagacag ttagataatt
     1561 tccacagcct cttatggtcg cggctcggat tatggttcac tctgagtcag ataggatcat
     1621 ggttaaaacc gagccgcaac cgtaagggag cggatagaac aacccattct attgtcaaaa
     1681 agcgtaaaaa atgactacaa tatacgattt ttcctggaca aatcctaggt atgttaaacg
     1741 aagcaatatc aaaaatgcca gaggcttttg aagctaaaat attgcttcgg ggtgaagagt
     1801 actttcaaaa aggccaggta ttgaatattc gcttaagtga cggcttgctc aaagggagag
     1861 tgaagggtag tgcaagtcaa atttatgata tccatatgga tttaaaatca tggccagcaa
     1921 aaccttctcg atgttcatgt gcttatcaat ataattgcaa acacgccgca gcttgtcttt
     1981 ttgcattaag agacagagaa aaaataaacc tgcagtcact gccttctaac aaactagatc
     2041 gaaaattaga tgtttggtta aaaaatttac gagcacaaga agcatcagaa ataaaaaggg
     2101 aggaggcgac acaccatctg atttatttga ttgatttaaa gcttcggggt tatgagcaca
     2161 aagtaagcat taaattagct ttggccaaac tattaaaacg cggcggttac ggaaaaaaaa
     2221 taatctttaa tactctggct aattcaaaaa aacaacattt tatgggtgat gataatgaca
     2281 tcgtggctca attgttattt aaatgtaatg tatccggttg gtttgataat cttttgattc
     2341 ggaatacaga gcttcttgat aagatcattg caactggaag agcttttttt attgaaaatg
     2401 aggatatccc tataaaaaag ggagagactt tgactggtga atgccagtgg attttatcct
     2461 ggaatggcaa tcagatttta aaattaaggc atgataatca gaatattgag ccattattat
     2521 tggatgaaag ttggtatttt gatattaaaa atgcggtaat aggtcactta aactctcctt
     2581 atcccatcaa gcagcttcgt catttgttgg aagcaccacc tatccctctt gatcaagcag
     2641 agttattggc caaaaaaatg tctaaaaatt gtcctgaatt tcccatgccc aatgtgttta
     2701 atcaaaagga aacgaaacaa ttaaaaccaa ttcctgtgtt aattctcgat gcggttaccg
     2761 aacaggaaga acacaggtgg attgatgacg gagaggaaaa taattctctt tatatagcaa
     2821 aaatcttttt tgattatgaa ggattgcttg ttccttttgc ggaagagtgt gaaaaagtgg
     2881 tgtctttaaa aggagatgta ttagttgaat accaacgtga tcaggaattt gaagatttaa
     2941 aaattcaaga gttgagaaat tcaattgatt tgaggccatt tcatcaatgg gaaaaatacc
     3001 attggaattt acccgatcac tcctttttta ttttaaaaga tgtgcaaatt gtttcagact
     3061 tggaaagtct ttatcagcaa tcgttacctt atttaaaaga ccatggatgg cgtatagagt
     3121 ttgcaagccc gttttatcaa gagattgttt ctgctgatga agtggaatgg tattccgata
     3181 ttcaggaaag tacgaccgat tttttttcct atcaattggg tattttggtt gaaggcaagc
     3241 cagtgagtat tgtgccgctg gttgcggatt tgattcaacg ttacaaaggg aatgatttag
     3301 ataatttacc tgatgagcag ctggttaaac tcccattgtt ggatggtcgt ttattgcaat
     3361 tgcccatggg gcgaattaag ccattggtcc gtttgttatt acagtttggc ttgcgccaaa
     3421 ttgatcaaaa tcagcaatta cagattaata aatatcaact ggtattaatg caggaagttg
     3481 aattggctat tgctgcgacc aaatcacgtt ggcaaggtgc tgaatcatta agagaagaat
     3541 taaggaagct ggtcagatta aatgatctgc cagaaataaa ggttccatct ggtcttcaag
     3601 ctcatttgag ggattatcaa cactatgggt taaattggtt acaatttttg cgggtcagtc
     3661 gttttaatgg cgtattagcg gatgatatgg gacttggcaa aacagtgcag actctcgccc
     3721 atttacaata tgagaaagaa caatcaagat tacataaagc cagtttaatc atagctccca
     3781 ccagtcttgt tggtaattgg tttgccgaag ccaagcgatt tacgccagag atcaaagtat
     3841 tgatttacca tggaagtgat agacatcagg acaattttga tgattatgat ttaattattt
     3901 ctacctacgg attaattcac agggacaaag aaaaatttgt tggatacccc ttttattatc
     3961 tcattttgga cgaagcgcaa ttcattaaga atgcacggac aaaaacaaca caaatcattc
     4021 aacaattaaa ggcttcacac cgtttatgtc ttactggaac tccactagag aatcatttag
     4081 gcgaactctg gtctttattt cattttctaa tgcccgggct attgggtgat gcaaagcaat
     4141 tcaggctttg gttcagaacg cctatagaaa aatatgccga tatgggaaga agggatttgt
     4201 tagcaaaaag agtgcaacct tttttactgc gccgaaccaa aaatcaggta gccaatgaac
     4261 tcccgcctaa aacggaaata actcgtacta tagagttggt tggtgctcag cgggatcttt
     4321 atgaggctat ccgcatgagc atggaaaaaa aggtgagaga tgccatagca aggcaaggat
     4381 tggggaaaag tcatatatta ttacttgatg ccttgttgaa attgcggcag gtttgttgtg
     4441 atccaagatt gttgtctatg tctgaagccg agatcgctca tggtacttca tgcaagctcg
     4501 atgcactgat ggagttgctt gataatttgg ttgaagaggg aaggagagta ttggtatttt
     4561 cacaatttac atcgatgcta aaactgattg aggaattatt aatagccagg caatacgatt
     4621 atttgaaatt gacgggacag acccaaaaca gacaagcctt agtcgagcaa tttcaacaag
     4681 gtgatacgcc aatattttta attagtctga aagcaggagg cacaggactt aatctgactc
     4741 gtgcagatac tgtgattcat tatgatccct ggtggaatcc ttctgttgag gaccaggcta
     4801 ctgacagaac acatcgaata ggccaggaaa gccctgtttt tgtttacaag ttgattacat
     4861 caggtactgt agaagaggca attcttggta tgcaagagaa gaaaagacaa ttagtggaag
     4921 ggattttgtc tacagacgct tccaggtcaa tggttttaag tgaagaagat ttgaatcaat
     4981 tttttatgcc tttatgaata aaagtaacgg tttattatcc gctccctggc ggtcgcggc
//
LOCUS       DBNCWK010000172         5020 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.173, whole genome shotgun sequence.
ACCESSION   DBNCWK010000172 DBNCWK010000000
VERSION     DBNCWK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.173"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            62..1138
                     /locus_tag="AC06IO_002668"
     CDS             62..1138
                     /locus_tag="AC06IO_002668"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HIO9875835.1"
                     /translation="MKNNELSLLEKQTVENLSLKASEYIEVARTRVQRSVNSEMVQAY
                     WLIGRDIIEAEQKGQIRATYGSHLLQELSHYLTKKYGKGFSVSTLKDIRQFYLAYPDL
                     QISHAVRGESIKAGLSLNLGWIHYRALMRIDRAEARQFYTVEAEKNCWSGRELERQIN
                     SLLYDRLAKSKDKEGLINLAKEGQEINKPEDAIKEPVVLEFLGLPESTKLVESELEEA
                     LINNLQQFLLELGKGFSFVGRQKRLTFDGDHFYADLVFYHVILKCYVIIDLKTRKLTH
                     ADLGQMQLYVNYFDKEIKQDDDNPTIGLVLCTEKSEEMARYMLGDKAKQIFASKYQLY
                     LPTEEQLEKEIRREVLILKQQKAE"
     gene            1218..1559
                     /locus_tag="AC06IO_002669"
     CDS             1218..1559
                     /locus_tag="AC06IO_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875836.1"
                     /translation="MPQLTLTLSHNINTDVINFKNLFNKIHEALRAVPNMDVNTAHSG
                     VIQESFSYIGFDNLKATKVYLQLYWMENEERAGMKPALGKTLLDILENNIVPEIASVG
                     RALARQFLELK"
     gene            1608..2138
                     /locus_tag="AC06IO_002670"
     CDS             1608..2138
                     /locus_tag="AC06IO_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIO9875837.1"
                     /translation="MQYRRALLPGSTYFFTVNLANRTNGILIKEIDLLLYAIRTTKAQ
                     YPFKIIAYVILPDHLHLIMILPEHDSNYSLRWSMIKSLFSRQIPCLELISTARKSKRE
                     RGIWQRRFWEHLIRDELDLEHHVNYIHFNPVKHGYVQKASDWKYSSIHRYIQKGIISS
                     DWACFDTLHTMNQFGE"
     gene            2479..3120
                     /locus_tag="AC06IO_002671"
     CDS             2479..3120
                     /locus_tag="AC06IO_002671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875838.1"
                     /translation="MILKHTNWYKKFLFLLGIVLSLKLMAIPPGEVVDACIESIAIDY
                     GMTFTEIAHPTGKEEELCEGGPAFDRECYMDMDIISENIHYGRSECRGVPSVFINGHN
                     LSLKNAQNFSINPYIKPGAPLSVHALWFKIGYRKKTYLCIVQPLSEFRVLTSGEQYYI
                     IENVMPFDIPVLYYYLFETDMMPLTMSWSEQEQIKKEFPFFKFSTEEDSDTPE"
     gene            complement(3303..3551)
                     /locus_tag="AC06IO_002672"
     CDS             complement(3303..3551)
                     /locus_tag="AC06IO_002672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875839.1"
                     /translation="MKTKIVPTFLYEGLGFPIELGNVEAVKFNGEWLPKIDLQYIADE
                     VIKKLAIQEERLTGNQVKFIRSYFSIPLSANGKVMGMK"
     gene            3724..4311
                     /locus_tag="AC06IO_002673"
     CDS             3724..4311
                     /locus_tag="AC06IO_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIO9875840.1"
                     /translation="MKTHNKLANIESLLEKPVFTSKEASELGMSPSLLCYYIKKDLIQ
                     RVNKGLYQAVSVKKEFDFKWEDLIFTVKSISQGVICLVSALVIYELTDEVPRKHWIAV
                     PHSTTKPMRTNTIIKRMRDVSTGLTTWEIDGESVPIFDMERTIIDAFRFLSKEIALKA
                     LQNGLREKHVDSRKLQKYAKKLRIDITPYLLAMTI"
     gene            4431..>5020
                     /locus_tag="AC06IO_002674"
     CDS             4431..>5020
                     /locus_tag="AC06IO_002674"
                     /inference="COORDINATES: protein motif:HMM:NF020419.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIO9875841.1"
                     /translation="MDDKLVFKGGFLLAQLVEIGRETTDLDFLARNIQAAQESIVALV
                     ETICTIELADGFIFEIMKVSDLEHAHMHYPGFRLSLRVKFYSMTDAIQLDIGVGDLVK
                     PVERSLLLTEVKNQALFESEITLLTYPIEFIFAEKLETLVSRGATNSRMKDYHDLLII
                     LRNYELNDFQNLQEVITNTFKHRGTELILPILFDRS"
BASE COUNT         1669 a          781 c          976 g         1594 t
ORIGIN      
        1 taatacggat cttaataaga tataattgtt gaattatcgc ggcgtcatga gactaccttt
       61 catgaaaaat aatgaattat ctttgttaga aaagcagact gttgaaaatt tatctttaaa
      121 agcttcagaa tatatagaag ttgctagaac cagagttcag cgttcagtca attctgaaat
      181 ggtacaggca tactggctca ttggcagaga tattattgaa gcagagcaga aaggtcagat
      241 tcgagcaact tatggaagtc atcttttgca agagctctca cattatttaa ctaaaaaata
      301 tgggaaagga tttagcgtta gtacattaaa agatattagg caattttacc ttgcttatcc
      361 agacttacaa attagccacg cagtgcgtgg cgaatctatt aaagctggct taagccttaa
      421 tttaggctgg attcattatc gtgcattaat gcgaattgac agagcagaag cgcgacaatt
      481 ttatacagtt gaagcagaaa aaaactgttg gagtggaaga gagcttgaaa ggcaaataaa
      541 tagtttactt tatgatagat tagctaaaag caaagataag gaaggattaa taaacctagc
      601 taaagaaggt caagaaatta ataaaccgga agatgctata aaggaaccgg ttgtattaga
      661 gtttttaggg ctaccggagt caacgaaatt agttgagtcg gaacttgaag aggctttgat
      721 taacaattta caacaatttt tattagaact agggaaaggt ttctcgtttg ttggacgtca
      781 aaaaagatta acttttgatg gcgatcattt ctatgctgac ttggtttttt accatgttat
      841 tcttaaatgt tacgttatta ttgatttaaa aacccgaaaa ttaactcacg cagatttagg
      901 gcaaatgcag ttgtatgtaa attacttcga taaagaaata aagcaagatg atgataaccc
      961 aaccatcggc ctcgtgcttt gtactgaaaa gagtgaggaa atggctagat atatgctagg
     1021 agataaagct aaacaaatat ttgcgagtaa gtaccaatta tatttaccga ctgaagagca
     1081 acttgagaag gaaattagaa gagaagtttt aattctaaaa caacaaaaag cagaatagac
     1141 atagagctat gtaaaaattt tattttatta tggcgtatat atcctcgcgt tggaaaattc
     1201 agtaggagaa ataaataatg ccacaattaa cacttactct ttcacataat ataaacactg
     1261 atgtaatcaa tttcaaaaat ctgttcaata agattcatga agcccttcga gctgtaccca
     1321 atatggatgt gaacaccgct catagcgggg ttattcaaga aagcttttct tacataggtt
     1381 ttgataatct gaaggcaact aaagtttatc ttcagcttta ctggatggaa aatgaggaaa
     1441 gagctgggat gaaaccagct ctaggaaaaa cattacttga tattcttgaa aataatattg
     1501 ttccagaaat agcaagcgta ggtcgggcct tggcccgaca atttcttgaa ttgaagtaat
     1561 aattgattta taaacgtagc caatggaaat gaatctggtc caaactcatg caatatcgta
     1621 gagcactctt gccgggatcg acctactttt ttacggttaa tcttgctaat agaacaaatg
     1681 gaattttaat caaggaaatc gacttgttgc tttatgctat acgaacaacc aaagcccaat
     1741 atccttttaa aataattgcc tatgtcattt tgcctgacca tttgcatctg ataatgattt
     1801 tgccagaaca tgacagtaac tactctttgc gttggagcat gattaagtct ctcttttcaa
     1861 ggcaaattcc ttgcctggag ttaattagca ctgcaagaaa aagcaaacga gaaagaggca
     1921 tttggcagag gcgcttttgg gagcacctga taagagatga attggatttg gagcatcatg
     1981 tgaattatat tcattttaac cccgtgaaac atggctatgt ccaaaaagca tctgattgga
     2041 aatactccag tattcataga tatattcaaa aaggaattat aagcagtgat tgggcctgct
     2101 ttgacacatt acatacaatg aatcagtttg gagaataaca ttgattcaag gcccgaccta
     2161 cgcttgttct ttgtaagcgc caaatagata atttccgtga atcaacattt tgaattatct
     2221 ctttattacg catatcattg agcaaattgc tgattttttg cttttgattt tcatttaatg
     2281 catcactaag cttgcctaat agtagcttat cgatatcact gcgtgtagca gattcgaagt
     2341 ttttaaagta atccacaatt aatttttgat aataggcatt atcttgtgct tgagtaacag
     2401 ggtgctctcg gggtgtattt ggaatgtgtt attatcattc aacttgtatc agcttaaaag
     2461 ctaataagtt aggttgttat gattttaaag catacaaatt ggtataaaaa atttttattt
     2521 ctcttaggca tagttttatc attaaagcta atggccatac cacccggaga agtggtcgat
     2581 gcatgtatcg aatccatagc aatagactat ggcatgacat ttacagaaat agcacatccc
     2641 actggaaaag aagaagagtt gtgtgagggg ggaccagctt ttgatcgtga atgttacatg
     2701 gatatggata ttatttctga aaacatccat tatgggcgat cagagtgccg tggtgtcccc
     2761 tcagtattta ttaatggtca taatttatct ttaaaaaatg cgcagaattt ttctataaat
     2821 ccctatataa agccaggtgc tcccttaagt gttcatgcac tgtggtttaa aattggatat
     2881 agaaaaaaaa catatctatg tattgtgcaa cctttatctg agttcagggt tttgacaagt
     2941 ggtgaacaat attacattat cgagaatgtt atgcccttcg atataccagt actttattat
     3001 taccttttcg aaactgatat gatgccctta acaatgagtt ggtctgaaca agaacagata
     3061 aaaaaagaat tccctttttt taaattttcg acggaggaag attctgatac cccagagtaa
     3121 acgccaccca ttgattcatg ttaagcgcag aggtttaatt gaagtggttt gggattttta
     3181 ccttttgcct ttacaaaagc tatagttaga ataaaaattc ttttgttggg ctttagtctt
     3241 agtttgagtt tgttcgataa tatagagccg taatacttgt tcggtatttt cattcatatt
     3301 ggttatttca ttcccatgac tttcccattt gcggacagcg gtatggaaaa ataggagcga
     3361 atgaatttaa cttgattgcc agttaatctc tcttcttgaa tggctaattt ttttattacc
     3421 tcatcggcaa tatattggag atcaattttg ggaagccatt cgccattgaa cttaactgcc
     3481 tctacgtttc ctaattcgat aggaaagcca agcccttcat acagaaacgt aggtactatt
     3541 ttggttttca tggtgagtgt ttccttgctt acattgagcg ccgtaataat aggcattatc
     3601 gtgcttatca ttaaagaaac caaagactga ttcacaataa tttttttggt tgatacacag
     3661 taaccagaaa tttaataaat aaacccattg gatttaattg ggtttatttg ttaaaatata
     3721 aatatgaaaa cacataataa gctcgctaac attgaatctc ttctagaaaa gcctgttttt
     3781 acaagtaaag aggctagcga acttggcatg tctccaagtt tgctttgtta ctacataaaa
     3841 aaagatctta ttcaacgagt caataaaggc ctatatcaag ctgtttccgt gaagaaagaa
     3901 tttgatttta agtgggagga tttgatattt acagtgaaat caatttccca aggtgttatt
     3961 tgcctcgtta gcgccctagt aatctatgag cttacagacg aagtgcctcg taagcattgg
     4021 atagcagtac ctcatagtac aacgaaacca atgagaacaa ataccattat aaaaagaatg
     4081 agggatgtaa gtacgggtct tacaacatgg gagattgatg gagaatcagt acctattttt
     4141 gacatggagc gtactatcat cgatgccttt cgatttcttt caaaagaaat tgctcttaag
     4201 gcactgcaga atggattgag agaaaaacat gttgattccc gaaaattgca aaagtatgca
     4261 aagaagctgc ggattgatat tactccttat ttgttggcaa tgacgatatg aatgaacaat
     4321 cacttaaagc aaaactgaaa taattagcat caataaagga aaagacattt caggagcttt
     4381 ggaaaatact ggcattagag cgttttctcg catgattagc caagtccaat ttggatgata
     4441 aattggtctt taaaggagga tttcttttag cacagttagt cgaaattggt agagaaacga
     4501 ctgacttgga ttttcttgca agaaatattc aagcggcaca agaaagcatt gttgcgttgg
     4561 ttgagactat ctgtactatt gaacttgcag atggtttcat ttttgaaatt atgaaagtga
     4621 gcgatttaga acacgctcat atgcattatc caggatttag gctctctcta agagtaaaat
     4681 tttattcaat gacagatgca attcagttgg atattggggt aggtgattta gtgaagccgg
     4741 ttgagcgttc gttactttta acagaggtaa aaaatcaggc tttatttgaa tcagaaatta
     4801 ctttattaac ctatccaatt gagtttattt ttgctgaaaa gttagaaact cttgtatcaa
     4861 gaggtgctac taacagtcga atgaaagatt atcatgattt gttaatcatt cttcgaaatt
     4921 atgaattgaa tgatttccaa aatttgcaag aagtgattac aaatacattt aaacatcgtg
     4981 gaacagagtt aatcctgcct attttatttg ataggtctga
//
LOCUS       DBNCWK010000173         4965 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.174, whole genome shotgun sequence.
ACCESSION   DBNCWK010000173 DBNCWK010000000
VERSION     DBNCWK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.174"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..2455
                     /locus_tag="AC06IO_002675"
     CDS             <1..2455
                     /locus_tag="AC06IO_002675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HIO9875842.1"
                     /translation="VAAESFEPVKIATIFHEIIKQSIIDNQISVIGLGYEVFNTLSLE
                     QLEGCLNNFLNYINTTTDNNKKYQYKMRDLVLKFVQERLRRNPAPEPQSLWTWLDFIG
                     EGSSHNLNQDDQIHAFLSKNMDYRRAVQTIAFATTEKLEQLWLILCTGYKGLWLNESD
                     IIFHLNNILTLQIPDADLRWKELVRWIFANQSFTGTAKDYAQKQSENNTHLFAHYKEI
                     EKRYLLAKEEMKKFEREQLQQKAKHQHKTLIRHKQYEEIRQYLNSGEHLNALYQVSMA
                     YLGWFSDITGETPQERVAELIGSELVHLALDGILSAIDKQKIPTVHEMINLRINSKQI
                     SPFEAILVVYCDILLSRGEKLDKLPLDLACSALLSCQQATYLKTKDQIADVQKQLELI
                     VFKDKTSRENFIKATIEPYLNCNEQYIPGLSRIETDVLFTDIAGKLALTWLEKYENLS
                     DNNLRVILNIAICHAQEKIIELIRYRINELRITKDSQWEVWLSAGFLLDFEYHFELLN
                     QQASEHKALIWPFGNMMFADELLSSYWPQVSEHQLYFFISKFAPVWEPVPYPSGSFLG
                     TENPWDATALIKRRINHLASIATSEAQQSLIKLINNTELKGYQCELKHALAQNIRLIS
                     ESNKKVYSLESIRNILSNREPANHEDLQALIIDELEMLQLRIKNSQTSDFEFYWNGDN
                     PHIENYCRNQLVSALIPYLERYKVRSHIEGVRSDDKRCDLLNTYNLLDVPIEIKGQWH
                     PDIWTAAENQLQDYSQDYHSDGYGIYLVLWFGDCKSKMPKSRNSKKPKTYQEMVDMLS
                     ENYKTLSKKTRIFVLDLSK"
     gene            complement(2523..3419)
                     /locus_tag="AC06IO_002676"
     CDS             complement(2523..3419)
                     /locus_tag="AC06IO_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HIO9875843.1"
                     /translation="MSNKLRSALYSVHQLIKQMQGYSYASKADMKHMINRCMKDLHEL
                     GFKVGHLNGLKPKHIYVLVEHWKKQGKSPATIKNYMSKLRKLAYFLDKPKLVKTGNDS
                     YQIDKRTYIPTQNKAIHHIDLNLCTDPHIRLSLEAQMLFGLRREESMKFVVSEAWHGD
                     CLYIKPSWTKGGIGRLLKITNEEQMKWLSKVWQQIRRGESLIPTDRTYKQHLGHYQQQ
                     AKLMGVCKLHGLRHAYAQRRYTELTKELDSLKVGLICPIAGGKPTKELNHEEKQIDRR
                     ARSIISRELGHSRMNITKTYLG"
     gene            complement(3409..4218)
                     /locus_tag="AC06IO_002677"
     CDS             complement(3409..4218)
                     /locus_tag="AC06IO_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HIO9875844.1"
                     /translation="MRKHSLRQIASQYLDHDNSGSPRGKKYRRFVILRMIEDLFVLGL
                     APSNWPGLTSLHLQQLIQHWHKKKVKPSTLMNYMTIIRKFLCHVGHNAENIDNLSLGL
                     QTKKIKKKTRKSPADIVEKINDPVAKVLLGFQIHFGLTLSEAMRILPGIHIQEHELLL
                     TREITFNSKDRKIPIRSEAQIKLIEDFNLLTQGNGCLISTHGYRAICFSWQKAMKSLR
                     LSGKKCWRYLYAQQLNSQLSSQISHYRLSLLIMDEMGLKSRTTLWSYLNEQ"
     gene            4299..4451
                     /locus_tag="AC06IO_002678"
     CDS             4299..4451
                     /locus_tag="AC06IO_002678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875845.1"
                     /translation="MPQGSKWWGKSRLMIILVQRFFSGLSPYCLNTKTHGNPTAAAAD
                     PWLIKK"
BASE COUNT         1624 a          828 c          925 g         1588 t
ORIGIN      
        1 ggttgcagca gaatcatttg agccggtaaa aattgcgaca atttttcatg aaataataaa
       61 acaatcaata atagacaatc aaatatccgt aattggttta gggtatgaag tatttaacac
      121 actgtctctt gaacaattag aaggatgctt aaataatttt ttaaattata tcaacacaac
      181 aactgataac aataaaaaat accaatataa aatgcgagac ctggtattaa aatttgtcca
      241 ggagcgttta aggaggaatc cggcaccaga gccacaaagt ttatggacgt ggctagattt
      301 tattggggaa ggctcatcac ataatcttaa tcaagacgat caaattcacg cttttttatc
      361 taaaaatatg gattaccgca gagcagtaca aacaatagct ttcgctacaa ctgagaaact
      421 tgagcaatta tggttaatat tatgtactgg ttacaagggt ttgtggctaa acgagagtga
      481 tataattttt catctaaata atattttaac tttgcaaata cctgatgccg atttgcggtg
      541 gaaagagctt gtacgttgga tatttgctaa tcaaagtttt actggcacgg ctaaagatta
      601 cgcgcaaaaa caatctgaaa ataacactca tttatttgct cattataagg aaattgagaa
      661 acgttattta cttgcaaaag aagagatgaa aaaatttgag agagaacaat tacaacaaaa
      721 agctaaacat caacataaaa ccttgattcg acataagcag tatgaggaaa tacgacagta
      781 tcttaattca ggagagcacc ttaatgcgct ataccaagta tcaatggcat atttaggttg
      841 gtttagtgat ataacaggag aaactccaca agagcgtgtt gctgagctta taggatcaga
      901 acttgtgcat cttgcgttag atgggatttt atctgctatt gataagcaaa aaataccgac
      961 agttcatgaa atgattaatc ttcgtattaa ttcaaaacaa atatctccat ttgaagctat
     1021 cttagtagtt tactgtgaca tattattatc tcgaggagaa aaattagata aattaccttt
     1081 agatcttgcc tgctcagctt tactatcttg tcagcaagct acttatttaa aaactaaaga
     1141 tcaaattgct gatgtacaaa aacaacttga acttattgtt tttaaagata aaacgtctag
     1201 agagaacttt attaaggcaa ccatagaacc ctacttaaat tgtaacgaac aatatatacc
     1261 tggcttatct agaattgaaa ctgatgtact atttacagat atcgcgggaa aattagcttt
     1321 aacatggctt gaaaagtatg aaaatttatc tgataacaac ttgcgagtaa tacttaatat
     1381 agcgatttgc catgctcaag aaaaaatcat tgaacttatc cggtatcgaa ttaatgagct
     1441 aaggataacc aaagattctc aatgggaagt atggctaagc gcaggtttcc ttttagattt
     1501 tgaatatcat tttgaactac ttaatcaaca agcttctgaa cataaggcac ttatttggcc
     1561 ttttggtaac atgatgtttg ctgatgaact tttgtcttcg tattggccac aagtttccga
     1621 acatcagtta tattttttta tttcaaaatt cgcaccagtt tgggaaccgg ttccttatcc
     1681 atccggaagc tttttaggaa ctgaaaatcc atgggatgcg acggcattga ttaaaaggcg
     1741 aattaatcat ctagcttcaa ttgcaacttc tgaggctcaa caatctctta taaaattgat
     1801 taataatacc gaattgaaag gatatcagtg cgaactaaaa catgctttag ctcaaaatat
     1861 cagacttatt tccgaaagca ataaaaaagt gtactcatta gaaagtattc ggaatatttt
     1921 atcaaatcgt gaaccagcaa atcatgaaga cttacaggca ttaataattg acgaacttga
     1981 aatgttacag ttacgtataa aaaatagcca aacaagtgat tttgaatttt attggaatgg
     2041 agataatccg catatagaga attattgccg taatcagctt gtttcagctt taattccata
     2101 tcttgaaaga tataaagtgc gttcgcatat agagggtgta agatccgatg ataaaagatg
     2161 tgatttatta aatacatata atttgttgga tgttcctata gagataaaag gtcaatggca
     2221 tcctgatata tggactgcgg ctgaaaatca actccaagat tattcacaag attatcattc
     2281 cgatggctat ggaatatact tagtattatg gtttggtgac tgcaaatcta aaatgccgaa
     2341 atcgagaaat agcaaaaaac caaaaacata tcaggaaatg gtcgatatgc tcagtgaaaa
     2401 ttataaaact ctatctaaaa aaacacgtat ttttgttctt gatctatcta aataattttt
     2461 cttcattgaa tttaaactga ggcgatgtct atcatttttg aacaatagtt cacattactt
     2521 ctttacccaa gataagtttt agtaatattc atgcgagaat gcccgagttc acggctaata
     2581 atggatctcg ctcgcctatc aatttgcttc tcttcatggt tgagttcttt tgtaggtttt
     2641 cctccagcta tagggcagat taatccaact tttaaagaat caagttcttt agtcaattcg
     2701 gtgtatcgac gttgagcata agcatgacgc aggccatgca atttacagac gcccattaat
     2761 ttagcttgtt gttggtaatg acctaaatgc tgcttatagg ttctgtccgt aggaattagg
     2821 gactcaccac gtcttatctg ttgccagact tttgataacc atttcatttg ctcttcattg
     2881 gtgattttaa gcaatcgacc aataccacct tttgtccaac tgggtttgat atataaacaa
     2941 tcaccatgcc aagcttcact gactacaaat ttcatggatt cttccctacg taggccaaaa
     3001 agcatttgtg cttcaagtga taggcgaata tgtggatcag tacataagtt taagtcgata
     3061 tggtggatgg ctttgttttg agtgggaata taagtgcgtt tatcaatttg ataagagtca
     3121 ttacccgttt taaccagctt aggtttatcc agaaaataag cgagtttacg caatttggac
     3181 atataatttt ttatagttgc tggactcttg ccttgcttct tccaatgttc aacaagtaca
     3241 tagatatgtt taggctttag gccatttaaa tgacctactt taaaacccaa ttcgtgtaga
     3301 tctttcatgc agcgattaat catatgtttc atatctgcct tactggcata ggaataaccc
     3361 tgcatttgtt taattaattg atgtactgaa taaagtgcac tgcgtagttt attgctcatt
     3421 gagataactc cagagtgtgg tacgtgattt caatcccatt tcgtccatga tgagtaagga
     3481 caaacggtaa tgggatattt gagaggataa ttgtgaattt aattgttggg catagagata
     3541 acgccagcat ttttttccag agagccttaa ggatttcata gccttttgcc agctaaaaca
     3601 gatagctcta tatccgtggg tgctgataag acaaccgttc ccttgggtta atagattaaa
     3661 atcctctata agctttattt gagcttcaga gcgaatagga atcttcctat ccttgctatt
     3721 aaaagtgatt tctcgggtca gtaataattc atgttcttga atatgtattc cgggcaaaat
     3781 gcgcatggct tcgctcaagg tcaaaccaaa atgaatttga aagcctaaaa ggactttggc
     3841 cacaggatca ttgatcttct ctactatatc agcaggggat ttccgagttt tcttcttaat
     3901 tttcttagtt tgtaaaccaa ggctgagatt atcaatattc tcagcattat gacccacgtg
     3961 acatagaaat ttgcggatga tggtcatgta attcataaga gtcgaaggct taaccttttt
     4021 cttgtgccaa tgttgaataa gttgctgcaa atggagggaa gtcaaacctg gccaattgga
     4081 tggcgcaagg ccaagaacaa agagatcctc aatcatccta agaataacaa agcgacgata
     4141 ttttttgcca cgagggctac cgctattatc atgatccaga tactggctcg ctatttgtct
     4201 taaagagtgt ttgcgcatag ttctattcaa tcaatttatt tgccctgtct ttggtttagt
     4261 gttaatcgag cgattcactc aaagaggata tccagcaagt gccgcaaggc tcgaaatggt
     4321 ggggcaaatc aaggttaatg attattttgg tacaacggtt ttttagtggt ttatctcctt
     4381 attgtttaaa tacaaaaacg cacggcaatc caacagcagc tgccgctgat ccatggttaa
     4441 taaagaagtg attattaact tatctacccg atggtagcct atcctagaac tacagattcc
     4501 caactagacg tccagccaca ggttagggga atgagagtgg cttagcataa gaccctaggg
     4561 tttcgcctga gttgcacagg ctcatcagtt atgcttatga aaaatttagc aaaggtgaga
     4621 tctgggagca ataggttttg gatgaatatt ttgttcaatc ctgtggtgtg ggattgaatt
     4681 ttgtttgtta attgtttgat tttaaaatta gatttatagc aagtttgttt gcgtcactgc
     4741 agaataattg cagtgacgca tagcttatca tttttcattt taattttttt aagacataat
     4801 ctaatttaga tttcatctga ttaataacgg aaggatccga aattgacaac aacgacatca
     4861 ttgtttcaag aaaatagttg gatatatatt gaagataaat ttattgctaa cgatatttca
     4921 ggtgaaattc ttgacaaacg tactttcaaa aaagtgtcag caaaa
//
LOCUS       DBNCWK010000174         4915 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.175, whole genome shotgun sequence.
ACCESSION   DBNCWK010000174 DBNCWK010000000
VERSION     DBNCWK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.175"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..501
                     /locus_tag="AC06IO_002679"
     CDS             <1..501
                     /locus_tag="AC06IO_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1124 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875846.1"
                     /translation="SKIIFNFQQLFNVERSTLLSHHKFNPKISNNREFLQDLEARYDR
                     LNQAIENNEPYPFLYGDVCLLKEYLQVILGYYQDQLKAHQPVANSYLKRITKSHKFST
                     LMSDISEEEHPELGKKDSEILIKYTINFCAKKIMMEDLKTISDLVIKPFLFDHKDEEG
                     FSYCNP"
     gene            739..1500
                     /locus_tag="AC06IO_002680"
     CDS             739..1500
                     /locus_tag="AC06IO_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIO9875847.1"
                     /translation="MKRIVFILLFISNFLVHSESLKVGVLQFAPPFSSISDKANHYYG
                     FVVALMNIICNRLQEKCEYIPIPKEGELKGLDKGVYHIVFSSTPITTSLPANYRYSLP
                     YLPSDGQFLTLKNNSIDTLNDINFKKVGFFKINFQQSPLLKKYETTNTLIEYTDPAEL
                     VNALTTKKIDAILINYQASRFIVNNLNPTEDDQLKLIGDKIPIGSGYGIIALKNQSAL
                     IDKINHILLEMEKDGSYLRIYNEYFGANPAYNPHH"
     gene            complement(1626..2954)
                     /locus_tag="AC06IO_002681"
     CDS             complement(1626..2954)
                     /locus_tag="AC06IO_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIO9875848.1"
                     /translation="MIKTFIDKKIQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKM
                     NHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIES
                     AYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
                     NGSWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMS
                     NPRAYKINLPAMPFRPYFEEVNIGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPY
                     KPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKL
                     NRLSDHSIRPNQILLIPSAKSTPALPFHEISKSKFLSQTPTKGKVYRVIHIVQANETY
                     NTLERLYKVTAQDIMKWNHIGPREALAKGRQLIIWKKSSNLEWNRTFKKP"
     gene            3317..3619
                     /locus_tag="AC06IO_002682"
     CDS             3317..3619
                     /locus_tag="AC06IO_002682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875849.1"
                     /translation="MGTFFEWFPREVGLDKNPDAFRTDCYKYYWGEPPRWGGTVDACE
                     VETDLYAMVYNSIGLLSYLIPLVGGYLALHFARNANSKAFSENNPEPGLEQSVLSP"
     gene            complement(3816..4586)
                     /gene="ceg19"
                     /locus_tag="AC06IO_002683"
     CDS             complement(3816..4586)
                     /gene="ceg19"
                     /locus_tag="AC06IO_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg19"
                     /protein_id="HIO9875850.1"
                     /translation="MFKKIITKLGIVMSKLRALEKEANELALILDQALLSQSIDVKKR
                     ALSQFENSELIQNKDNLRVFIDSEHPIVSLIRLATKILPEKIENLQKKVRLLEIQLAA
                     ETEETLDESNSDSESVDYDNGIDVETVVPVTVTHQEHVSEPDTRSVAIARLREVLKPY
                     IDSTQERTYEHYYGMAGTLFSFFGAAGYSKTDKLNAITKLFQNLEGDGAEPLNDTDRD
                     ILTTGVLGTTLKPLLEDEHIGNELKDLLSIKTDNVNTI"
BASE COUNT         1536 a          923 c          859 g         1597 t
ORIGIN      
        1 tcaaagatta tctttaattt tcaacaattg tttaatgttg aaagaagtac tcttctttct
       61 catcacaagt tcaaccccaa aataagtaac aatcgagaat tccttcaaga tctggaagct
      121 cgttatgatc gcctcaatca ggcaatagaa aataatgaac cttacccttt tttatacggc
      181 gatgtatgtt tattgaaaga atacctccaa gtcattctag gctattacca ggatcagctt
      241 aaagcacatc agccagtagc aaacagctat ttaaaacgga taactaaaag ccacaaattt
      301 tcaactctaa tgtctgatat ttcagaagag gagcatcctg agcttggcaa aaaagattcg
      361 gaaattttaa tcaagtacac cattaacttc tgtgcaaaaa aaatcatgat ggaagatcta
      421 aaaacaatta gtgaccttgt aataaaacca tttttatttg atcacaaaga cgaagaaggt
      481 ttttcttatt gcaatccata agctggagaa tagattagag ggcagtgacc tggatttaac
      541 gatatctcta aatcggatat actgcccaac cccttccaag ccctaccttg aaaagcctct
      601 tgattgcgca gctaacaata ttttatttca ctaaaattga ctctgaaaaa ccgatccaaa
      661 atgaaaacta ttgccctata attaaaaaag attacttctc ttattaaaaa tggaccagat
      721 taagaagaga cccaatttat gaaacggata gtatttatac tgttgtttat aagcaatttt
      781 cttgttcata gtgaatcact caaggtgggt gtcttacaat ttgctcctcc tttctcatct
      841 atatcagaca aagcaaatca ctattatggt ttcgttgttg ccctcatgaa tataatatgc
      901 aaccgtttgc aagaaaaatg cgaatacatc cctattccta aagaaggcga gttaaaaggt
      961 ctggataaag gtgtttatca tattgttttt agctcaaccc ctatcacgac atcattacct
     1021 gctaactatc ggtacagctt gccctactta cccagtgacg gtcaatttct gacattaaaa
     1081 aacaactcga ttgatacctt gaacgatatc aattttaaaa aagttggttt ttttaagatt
     1141 aattttcagc aatctcctct tttaaaaaaa tacgaaacca ccaatacgct tatagagtac
     1201 acagatccgg cagaattagt taatgcacta acaaccaaaa aaattgatgc gattttaatc
     1261 aattatcaag cgtcacgatt catcgtgaat aatctcaacc ccactgaaga cgatcaatta
     1321 aaattgattg gcgataaaat accgatagga agcggatatg ggatcatagc ccttaaaaac
     1381 caatcggcat tgattgataa aattaatcac attttgctgg aaatggaaaa agatggttcc
     1441 tacctccgta tctacaacga atattttggt gcaaatccgg catataatcc tcatcattag
     1501 caggttagtg tgtaaatcgc cacatatgag aaatttaacc aaatttatta ataagttatc
     1561 gaataggcaa gcgtgaaact ttgaccgaac acgggttatg tttagaatgc aggattagcg
     1621 gaaacttaag gttttttgaa agtcctgttc cattcaagat tgcttgactt tttccaaata
     1681 attaactgtc gcccttttgc caacgcttct ctcgggccaa tatgattcca tttcatgata
     1741 tcctgagctg taactttata aagtcgctca agagtattat acgtttcatt ggcttggaca
     1801 atatgtatga ccctgtatac tttacctttt gttggtgtct ggcttaagaa ttttgacttt
     1861 gaaatttcgt ggaaaggcaa tgctggagta cttttggcgc tcggaattaa caagatctga
     1921 ttgggtctta tcgagtgatc tgacagacga tttaattttt ttattaatga aacagtcgta
     1981 tgatattttc tggcaatact ttgtaaactg tcactcggaa ggacgagatg ttttctccag
     2041 ctcactcgtt tatcaacagg tgaatgggat aaattgaaat aaaattgagt cacttttgct
     2101 atagggataa gcaaagtaaa gggtttataa ggtgcggtag cccatctatt ataacccgga
     2161 tttaatttgg ttaattcact aaccgaaatt cctgctaaac tagccgcatg attcaagtca
     2221 atttgactgc caatattcac ctcttcaaaa taaggtcgaa atggcattgc aggaagattt
     2281 attttataag cccgagggtt gctcatgatt tcagccaaag ctaaaaatcg agggacataa
     2341 atttgagttt ctttaggtac atccagattc caaaaactga cgctacctcc gttagtgtta
     2401 tttgctttta cagctctgcc tatggctcct tcgccagcat cgtaagctgc gatggaaagt
     2461 atccagcttc cgttaaacac tttatttaaa tgcatcaggt gagtcagggc tgcatcagtt
     2521 gaatgattaa tgcttcgtct tccatcaaac caccaatctt gaattaaacc taattgattc
     2581 cccgtcaccg gcattatttg ccataaacct gcagcaccaa cttttgagta agcaaaagga
     2641 tcgtaagcgc tttctatcat agggagtaat gccaactcac cgggtaaatt tcttctttgt
     2701 atttctcgag ttacatgata aagataaggc tcagcctgtt tacaggcctt agtcacaaac
     2761 cctggatgat ccagtagcca gcgaatttga tcttgaacct ctgggcgata ggcttcatga
     2821 ttcatcttga attcactgcg caaaacatcc catacatcag gtgtttcaaa tgatttagca
     2881 tgagtactga agataaaaaa aataaggcaa ctgaatagca gctgtatttt tttatcgatg
     2941 aatgtcttaa tcaaattact attcctgctc tataaagctt ctctggagcc cgtttataat
     3001 gtattgtttc tcttcgcctg aaccggccct gagcgcgtct tataaaatat actcctctta
     3061 gtatcttgta taaagcactt cctgcaatat aaggccatga taatttaaca cccggtctat
     3121 ttttaacgtt tttttattaa tattgctcta acatttgttt cgatatatgc tgatggctcc
     3181 tccagcaatt atttatgaca aactgatttt tatcagagcg gttagggttt attttcatat
     3241 ggaaatggga tggctggttg tgtgtatcaa gcatcaagga atgataagaa ttaatatatt
     3301 caggagattg taaattatgg gcactttttt tgaatggttt cccagagaag taggtttaga
     3361 taagaacccg gatgcattta ggacagattg ctataaatac tattggggag agcctcctcg
     3421 atggggtggc accgttgacg catgtgaagt tgaaactgat ttatacgcta tggtatataa
     3481 ttccatagga cttttgagct acttgatacc tttggtaggc ggatatttag cattgcattt
     3541 tgcacgaaat gcaaactcca aagcattttc cgagaataat ccagaacctg gccttgaaca
     3601 gtctgttctt agcccttaaa taagcttaac tatttctaaa aatcaataat ttaaaaatcc
     3661 agataaaaag atacgctttt aaatcgaatc ccgttataag attttcatat atcaggaatt
     3721 ttgcctaaga tgccggatca ttgatccggc atcggatggc ttgatgagat aagtgtttca
     3781 gatgaacagg aaaactcttg ttcaaatatg agtttttaaa ttgtattcac attatcggtt
     3841 tttatcgata gcaagtcttt caattcatta ccaatatgct catcttctaa caacggcttc
     3901 agagtagtgc caagaactcc tgtagtcagt atatctctat ccgtatcgtt gagtggctca
     3961 gcgccatccc cctcaagatt ttggaataat ttggtgatag cgtttagttt gtccgtttta
     4021 ctgtaacctg ccgctccaaa aaaactgaac agtgttcctg ccattccata atagtgttca
     4081 tatgtccttt cttgagtaga atcgatataa ggtttcaaca cttctcttag tcgtgcaata
     4141 gccacagacc tggtgtctgg ctcactgaca tgctcttgat gagtaacggt gacaggaaca
     4201 acagtctcta catcaatacc gttatcgtaa tcaacgcttt cagaatccga attcgattca
     4261 tcaagggttt cctcagtttc agcagccaat tggatctcga gcaaacgtac ctttttttgc
     4321 agattttcta ttttttcagg caaaattttt gtagcaagtc tgattaagct tacaatgggg
     4381 tgctcagagt caataaaaac ccgcaagtta tctttgtttt gaattaattc tgaattttca
     4441 aattgagata atgctctttt tttaacatct attgactgac tcaacagagc ctgatcaaga
     4501 atgagagcca attcattcgc ttctttttcc aatgctctta gtttagacat aactattccc
     4561 aatttagtga taattttttt aaacagtgat taattataaa acaaaaatat taagataatc
     4621 ttaacttatg aaaaatgggc tattttcccg attcgtaaat tttccttcgg aatggtactt
     4681 gatagagctt ttgtaaaata gttttacgga agtgttctca agttccattg gtttgacttt
     4741 tgtcttcttg cggttgatgc cattcataac caaataagtc agagtattat gagattctgc
     4801 ctttttaaat taaacagagt tcatgattta tctattggta ctgaaaattc ggataacatc
     4861 tacagtgata aatttatgta ttatttttga tcactaaaaa ccttaaatag agtta
//
LOCUS       DBNCWK010000175         4820 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.176, whole genome shotgun sequence.
ACCESSION   DBNCWK010000175 DBNCWK010000000
VERSION     DBNCWK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.176"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            230..319
                     /locus_tag="AC06IO_002684"
     tRNA            230..319
                     /locus_tag="AC06IO_002684"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:264..266,aa:Ser,seq:gct)
     gene            326..402
                     /locus_tag="AC06IO_002685"
     tRNA            326..402
                     /locus_tag="AC06IO_002685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:360..362,aa:Arg,seq:acg)
     gene            complement(419..1702)
                     /gene="legL2"
                     /locus_tag="AC06IO_002686"
     CDS             complement(419..1702)
                     /gene="legL2"
                     /locus_tag="AC06IO_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL2"
                     /protein_id="HIO9875851.1"
                     /translation="MRVELFNPSIKKIIVKLSQLKGLWIVQLNLSKNRLSEKKFTDLV
                     VLLSMLKAFGVTYLNLHFNNLGQKTGTELVALFKILKDSGISRLSLSANELYQKTGTE
                     LATLLTVLSDSNIIQLDLSCNWLGQRPDVELIELFKAWSSTPIIDLDLANNDLGKKSG
                     SVLSEMFRALKVSLISHLGLRNNDLYQRTGAEFSSLLSAIKESPITHLDLSDNGLHLM
                     VAAFETLFDALKDSSVTHLNLCRNGIGHLDMFQMTAMFCSLQCTGVTHLDLGENSLEL
                     MTAEELAAIFQVFKDSRLIHLGLNDSYLNNRTTEDLHLIFNALPANIATIKLSLEELT
                     EMSSKQRQTIQRRFPRAEQVILVNNVEGRELNPSLSLGDANVYRRLGFVGASPPSLAG
                     LASFFMVSNNLRFTREVQKLLPDEVTERIVKNILG"
     gene            2177..3601
                     /gene="plaB"
                     /locus_tag="AC06IO_002687"
     CDS             2177..3601
                     /gene="plaB"
                     /locus_tag="AC06IO_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_341574636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase PlaB"
                     /protein_id="HIO9875852.1"
                     /translation="MIVIFVHGWSVTHTNTYGELPQWLENQSKQGKLDIQVGNIYLGR
                     YISFDDTVTVDDIARAFDQAVRDEIADKVKDGQRFACITHSTGGPIVRKWMDLYFKNN
                     LAKCPLSHLIMLAPANHGSALAQLGKSRLGRIKSFFEGIEPGKCVLDWLELGSDMSWQ
                     LNESWLDYDCTANGVYSFVLTGQKIDRQLYDAVNSYTGESGSDGVVRVAATNMNYSLL
                     KLHQEGDNGESLVVAKMTRTQPMAFGVLPGLSHSGKNIGIIRSITMANAATHPTAIWV
                     LRCLQVKSRDSYNKLVKELDNITKETQKNEHKEFVKTLVFTREYITNRYSMIIFRLID
                     DRGNHLLDYDLYLTAGPQYSEQALPAGFFVDRQRNLNNRGKLTYFLDYDIMEGGINTP
                     KMQGNLGFRVKAYPESSEQALAYYRLLDFHSSLADIHKILHPNETVMVEIMLQRRVDR
                     TVFRINNNLTPAKISGKPTGKKID"
     gene            complement(3672..4349)
                     /locus_tag="AC06IO_002688"
     CDS             complement(3672..4349)
                     /locus_tag="AC06IO_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HIO9875853.1"
                     /translation="MYSYIDFNDKVHRLIPSKFPPVTLFDWADSAEELEQIALLEGLT
                     NERILSELGKINLIDQEDWIGGPGSTPIMAAFTHTGFESRFSDGSFGIYYAASSLETA
                     IKETCFHRERFYSASNEKPCSISMREYVANIKKPLIDITDKSYKDLFNPDPACYSKSQ
                     EFGKKICEKKEWGLLYPSVRDLNGLCVAVLRPPALTIPIQGCHLRYIWDGEKISDIYK
                     ESKITNI"
     gene            complement(4351..4818)
                     /locus_tag="AC06IO_002689"
     CDS             complement(4351..4818)
                     /locus_tag="AC06IO_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HIO9875854.1"
                     /translation="MNEHLIHLICLFVDNCLFGDYTIVESSQEFSMQTNTRSLQSIPD
                     EVVWKSLTNLVERFDLKESEARILMGDMPRSTYTSHKAKLNRDQKERVSYLLGIYKSL
                     RILFEDGEQARTWINRKNDLPPFKGLTPKEYMLEGGMVRLADVRKFLDFWRGY"
BASE COUNT         1478 a          978 c          887 g         1477 t
ORIGIN      
        1 ggacaaattg tttcacttaa atattccaat agagtgtcat accacataat aatcctttaa
       61 atccctttaa taggtagtgt cataacataa atacattaaa aaaaaaatct gctgatattt
      121 ttatatgcat taatgatatt tattatgctc aattttgaat ttcatcactt tatgcaataa
      181 gaattattga cgcatgttaa atacctgata tactccgcta ccattgattg gagagatggc
      241 cgagtggtcg aaggcgctcc cctgctaagg gagtatggga gaaatcccat cgagggttcg
      301 aatccctctc tctccgccaa tcaacgcgcc cgtagctcag ttggatagag tatctggcta
      361 cgaaccaggg ggtcggaggt tcgaatcctt ccgggcgcgc cattttttta cggcctattc
      421 agccgagaat attcttgaca atacgttctg ttacttcatc cggtagtaac ttttgcactt
      481 cacgcgtgaa cctgaggttg ttgctgacca taaaaaagga agctaaacca gctaaagacg
      541 ggggagacgc cccaacaaag cccaaccgcc gatatacatt ggcatcaccc aggcttaatg
      601 aagggtttaa ctctctaccc tccacgttat taactagaat aacttgctcc gccctgggaa
      661 aacgcctttg aatcgtctga cgttgcttgc tgctcatctc agttaactcc tcaagcgata
      721 atttaatcgt cgctatattg gctggcaatg cattaaaaat aagatgcaaa tcttctgttg
      781 ttctgttatt taaatagcta tcatttaaac caagatgaat taatctggag tctttaaaga
      841 cttggaatat agctgctaac tcctcagcgg tcatcaactc cagactattc tcgcctaaat
      901 caagatgagt cactcctgta cactggagtg aacaaaacat cgctgtcatt tgaaacatgt
      961 ccagatgccc aatgccatta cggcataaat tgagatgggt gacggaggag tcttttagag
     1021 catcaaatag agtctcaaaa gcagcaacca ttagatgcaa tccattatca cttaaatcga
     1081 ggtgagtgat tggagattct tttatggcac tcaacaggga tgaaaattcg gcacctgtcc
     1141 tttgataaag atcattatta cgtaaaccaa gatggctaat taaagatact ttcaatgcac
     1201 ggaacatttc cgacaacacg ctccccgatt ttttaccaag atcattattg gccaagtcaa
     1261 gatcaatgat tggagtacta ctccatgcct taaatagttc gattagttca acgtctggtc
     1321 tttgtcccag ccaattgcag cttaaatcaa gctgaatgat atttgaatcg cttaaaacgg
     1381 ttaacaaggt ggctaattcc gtcccggtct tttggtaaag ctcattagca cttaaactaa
     1441 gacggctgat tccagagtcc ttaagaatct taaacagggc taccaattca gttccagtct
     1501 tttgcccaag gttattgaaa tgcaaattaa gataagtgac tccaaaagcc ttgagcatac
     1561 taagcagaac taccaagtca gtgaattttt tctcactcag tcggtttttt gacaaattga
     1621 gttggacaat ccacaagcct tttagttgac tcaatttgac aatgattttt ttaatggatg
     1681 ggttaaatag ttcaacgcgc atggatgctt cttatattac tttgcaaggc taaaaaaagc
     1741 gaatacgaac tgatttacaa aagctatgtc ttttcacctt aatctgtcct aatctttctc
     1801 gccgtgcaat attgtactat atcacccatc catatccata atatcttatg gactataatt
     1861 ttcaaaactt caaagccgca taagcagtcg ttgaatcaac tagaaatttt gtataattca
     1921 tgtataaata atcgatttcc tttcagttaa ttaattagaa taaaattgat gaaaaatatt
     1981 tcaaaaagat atcattcaag cttatattgc tcttgtcttt taacagtagg ctaaatattc
     2041 attaaaaggc tgcttatttt ttaatactga aaactctggg tcataattaa aaacttgagg
     2101 gttgagtata aaataatcta atatcatcaa gttactcaac aatgtcttgt tgtgagccaa
     2161 caattcaagg agcgttatga ttgttatctt cgtccatggc tggagtgtca cgcataccaa
     2221 tacttatggg gaacttcctc aatggcttga aaatcagagt aaacagggaa agctggatat
     2281 tcaagtcggc aatatttatc ttgggcgcta tattagtttt gatgatacgg tgacggttga
     2341 cgatatagca cgcgcatttg atcaagcggt acgtgatgaa attgctgata aagtaaaaga
     2401 tgggcagcgt tttgcttgta tcactcactc taccggtgga cccattgtcc gcaaatggat
     2461 ggatttatac tttaaaaata atcttgcaaa atgtccgttg agtcacctta tcatgctggc
     2521 tcctgccaac cacggttctg ctcttgctca acttggtaaa tcccggctag gccgcataaa
     2581 gagttttttt gaaggcattg aaccgggaaa gtgtgtactt gattggcttg aactcggaag
     2641 cgatatgagc tggcaactta atgaaagctg gcttgattat gattgtactg caaatggtgt
     2701 ctattccttt gtacttactg gccagaaaat tgatcgccaa ctttatgatg cagtgaactc
     2761 ctacacaggg gaatccggat ccgatggggt ggtacgcgtt gctgcaacaa atatgaatta
     2821 tagcttattg aaattacatc aggaggggga taacggtgaa agccttgttg tcgccaaaat
     2881 gacacgaaca cagccaatgg catttggagt tctacctgga ctttcacatt ccggtaaaaa
     2941 tatcggtatc atccgtagta ttactatggc caatgcagcc actcacccca cggcgatatg
     3001 ggtcttgcga tgtctgcaag taaaaagtcg tgactcctat aataaattag taaaagagtt
     3061 ggacaatatt actaaagaga ctcagaaaaa tgagcacaag gagtttgtga aaacacttgt
     3121 ctttacgcgt gagtatataa ccaatcgtta ctctatgatt attttccggc ttattgatga
     3181 ccgcggcaat caccttctcg attatgatct ttacctaact gccggacctc aatatagcga
     3241 gcaggcgctt cctgcaggtt tttttgtaga tcgtcagcga aatctcaata atcgaggtaa
     3301 actgacttat tttcttgact acgatatcat ggaaggtgga attaataccc caaaaatgca
     3361 gggtaatctt gggttccgtg ttaaagcata tcctgaatca agtgagcaag cccttgccta
     3421 ttacaggtta cttgattttc attcatcgct tgctgacatt cacaaaatac ttcatccgaa
     3481 tgaaaccgta atggttgaaa tcatgctgca aagacgagta gataggacag ttttccgtat
     3541 caacaacaac cttactccgg caaaaatcag tggaaagcca actgggaaaa agatagattg
     3601 atacctcttt cgtttaatgc atttgcatca ttaatgagaa agtcatctag ccacaacttg
     3661 ggaaaaatat cttaaatatt ggtaatttta ctttctttat aaatatctga aattttttca
     3721 ccatcccaaa tatatcttaa atggcatcct tgtatcggta tagtgagcgc gggaggtctt
     3781 aatacagcca cacataagcc atttaaatct cttacacttg ggtatagcaa gccccattcc
     3841 ttcttttcac aaattttctt gccaaattcc tgacttttac tataacaagc agggtcagga
     3901 ttaaacaagt ctttataact tttatctgta atatctatta atggtttttt tatattagct
     3961 acatactctc tcattgagat ggagcaaggt ttttcatttg atgcgctata aaaccgttct
     4021 cgatgaaagc aagtttcctt tattgctgtc tcaagagatg aagcggcata ataaatacca
     4081 aaactgccat cactaaatct tgattcaaat ccagtatgtg taaaagcagc cataattgga
     4141 gtagatccag gaccaccaat ccaatcctct tgatctatca aatttatttt acccaactct
     4201 gaaagtatcc tttcattggt taagccttca agtagcgcta tttgttcgag ctcttctgca
     4261 gaatctgccc aatcgaataa agtcacggga gggaatttag aaggtattag tctatgcact
     4321 ttatcattaa aatctatata ggaatacatc ctaataacct cgccaaaagt ccagaaactt
     4381 ccttacatct gcaagacgaa ccatcccccc ttccagcata tattctttgg gtgttaaacc
     4441 tttaaacgga ggtaaatcgt ttttacgatt aatccaagtt ctggcttgtt ccccatcttc
     4501 aaacaatata cgtaaagact tataaattcc taaaagatag gagactcgtt ctttttgatc
     4561 cctgtttagt ttcgctttat gggaagtgta cgtagagcgt ggcatatccc ccatcaaaat
     4621 acgggcttca ctttctttta aatcaaaacg ttctacaaga ttggttaaag acttccatac
     4681 cacctcatct ggtatgcttt gtaaagacct tgtattagtt tgcatactaa actcctgact
     4741 gctttctact atagtatagt ctccaaagag acaattgtct acaaaaagac atattaagtg
     4801 aattaaatgc tcattcaatg
//
LOCUS       DBNCWK010000176         4760 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.177, whole genome shotgun sequence.
ACCESSION   DBNCWK010000176 DBNCWK010000000
VERSION     DBNCWK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.177"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1124
                     /locus_tag="AC06IO_002690"
     CDS             <1..1124
                     /locus_tag="AC06IO_002690"
                     /inference="COORDINATES: protein motif:HMM:NF024700.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIO9875855.1"
                     /translation="KQSHRQKMTKISSITIKGFKSILSLDQFALNDLNVLIGANGSGK
                     SNFVSYFEMLGELIEQRLQIWVKKQGGSDRLLSFGIKTTKQIESSIRFGSNGYDLILE
                     PTDNGEFVFSQEQLYFDGPYYDITKPRLGSGHSETNIIDEMKHGTSQKIATFCYHAIA
                     GWKVYHFHDTSKTAGVKRYGALHDNEYLRGDASNLAAFLYRMKKENPGAYQQIIKVIQ
                     LAIPFFDDFILRPRKLSTGEEQINLLWKQKDSDYPFWPSQLSDGSIRFICIATVLLQP
                     KAPTTIIIDEPELGLHPYAIALIASLIRSAANRMQIIISTQSIPLINEFSINDLIVVE
                     RENGASLFKRHNKIDFQEWIKEYSLGELWEKNILGGKPR"
     gene            1124..1789
                     /locus_tag="AC06IO_002691"
     CDS             1124..1789
                     /locus_tag="AC06IO_002691"
                     /inference="COORDINATES: protein motif:HMM:NF025468.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HIO9875856.1"
                     /translation="MKRLHIICEGHTEELFVNKLLIPSMQAKGVLITPSQIGKPGHKG
                     GNVNYNRLLFDVKNRVLSDKNCYCTILLDYYGLPSDFPGKQDSTKYRSSLEKHNAITK
                     ALQKKMEVDIKEASKRFIPYIQLHEFEGLLFSSPNSITKILQQPNLLIEFEKIQKKYE
                     TPEDINDSPETAPSKRIQALHKEYDKPLHPVLIAEDIGLDMIREKCPLFNSWVNRLEE
                     LGN"
     gene            complement(1910..3433)
                     /locus_tag="AC06IO_002692"
     CDS             complement(1910..3433)
                     /locus_tag="AC06IO_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012276301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HIO9875857.1"
                     /translation="MIIMDSKTQLTVDVITKVSEGKITINNAAKLLNCSRRTIERYLN
                     RYQKFGIKFAIHGNTGRPPVNKTSDNLKLKVQSLIKEKYYDVNLQHLAELLNLNENIY
                     IKRETLRTWAHDIHHVKRAKRRRSQVRKKRERMESSGLLLQMDGSSHQWFGDKKSCLI
                     AIIDDATSEIHAEFFKSETTQGCLKVLRDYIEKKGIFKTLYVDRAGIFGGPKRCNFSQ
                     MQRACEELGIEIIFANSAQGKGRIERSFDTFQDRLIPELRLQNISDMQSANVYLQEFF
                     IPEFWQKNLTVNPKNVTSEFVSVPRHLNLDDICVQKDYRKIRNDHTFSYGNKFYLIES
                     PLKHSIAKQEIEIRKQPNGSFIAYFAGRHLVVSEVIEPTKPSMYDLEIQKKIDAIELA
                     KKLGNVSEAARISGCSRETIYKNRRLLMEKGPLALKRTFQPDIRHKNRTTEDVEETVI
                     GFSLINPHLGQAQVSAQLKTYYDIEISPEGVRYIWLRENMNTCALRVLKAKSSPKAV"
     gene            complement(3591..4650)
                     /locus_tag="AC06IO_002693"
                     /pseudo
     CDS             complement(3591..4650)
                     /locus_tag="AC06IO_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035718769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         1490 a          861 c          870 g         1539 t
ORIGIN      
        1 caaaacaatc tcataggcaa aaaatgacta aaattagctc gatcacaatt aaaggattta
       61 aatcaattct ttcattggat caatttgcct taaatgactt aaatgtcctc attggtgcca
      121 atgggtcagg taaaagtaat tttgtttcat atttcgaaat gctgggagaa ttaatagagc
      181 aacgtttaca aatatgggta aagaaacaag gaggttctga tcgactcctc agttttggta
      241 ttaaaacaac aaaacaaata gaaagtagta ttcgatttgg aagtaatggt tatgatttaa
      301 ttttggaacc aacagataat ggagagtttg ttttttcaca agagcaatta tattttgatg
      361 gtccttatta tgatattaca aaaccaagat taggctctgg tcatagcgaa actaacatta
      421 tagatgaaat gaagcatggg acatcacaaa aaattgcaac gttttgttac catgctatag
      481 ctggctggaa agtttatcat ttccatgata cgagtaaaac agccggggta aaaagatacg
      541 gcgcacttca tgacaatgag tatttaagag gagatgcatc gaatcttgca gcttttcttt
      601 accgcatgaa aaaagaaaat cctggtgcat atcaacaaat tataaaagtt atacaactag
      661 ctatcccttt ttttgatgat ttcatattac ggccccgtaa attatcaact ggagaggaac
      721 aaataaattt actgtggaaa caaaaagaca gcgattatcc attttggcca agccaattgt
      781 ccgatggttc tatcagattt atttgtattg ctacagtttt gctacaacca aaagctccta
      841 caacaataat catagatgaa cccgaattag gtttacatcc ttatgctatt gctctaatag
      901 catcgttaat aagatcagca gctaaccgta tgcaaataat tatatcaaca caatcaatac
      961 ctcttattaa tgaattttca ataaatgacc tgattgtggt tgaaagagaa aatggagcat
     1021 ctctatttaa acgccataat aaaattgatt ttcaagaatg gattaaagaa tactctttag
     1081 gcgaattatg ggaaaaaaac atcttgggag gaaaaccaag ataatgaaac gcttacatat
     1141 aatatgcgaa ggccatacag aggaattatt tgtcaataaa ttgctaatac ctagtatgca
     1201 agcaaaaggc gtattaatca ccccatcaca aataggaaag cccggccata aaggcggtaa
     1261 tgttaattac aatcgtttgt tattcgatgt aaaaaacaga gtgctgtctg ataaaaattg
     1321 ttattgtaca attttattgg attattatgg attaccgagt gattttccag gtaagcagga
     1381 ttctactaaa tacagaagca gtttggaaaa acacaacgcc atcactaaag cacttcaaaa
     1441 aaaaatggaa gttgacataa aagaagcgtc gaaacgtttt ataccttata ttcaacttca
     1501 tgagttcgaa ggtttgttat ttagctcacc aaactccata acaaaaatac tgcaacaacc
     1561 gaatttactg attgagtttg aaaaaattca aaagaaatat gaaacgccag aggatatcaa
     1621 tgacagtcca gaaactgcgc caagtaaacg catacaagcc ttacataagg aatatgataa
     1681 gcctttacat cctgtattga tagctgagga tattggttta gatatgattc gtgaaaaatg
     1741 tcctctattt aatagttggg ttaataggct cgaggaactg ggaaattaaa gtattcaata
     1801 tggttgagta agttgaatta gtgtgattac cgagggaaac tgacggacta attaccaagc
     1861 gactttgtcg ttatacaata taagttgaag atgagtcaat caatcctcac taaactgctt
     1921 ttggtgagga ttttgccttt aggactctaa gagcgcaagt attcatgttt tcacgaagcc
     1981 agatatacct taccccctcg ggactaatct caatatcata gtatgttttt aattgcgcag
     2041 aaacctgcgc ttgtcctaga tgcgggttta ttaatgagaa gccgatgaca gtttcctcaa
     2101 cgtcctcggt ggttctattt ttgtgtcgga tatcaggttg aaatgtccgc tttagagcaa
     2161 gagggccttt ctccataagt agtcgcctat ttttataaat ggtttcacga gaacaaccgc
     2221 tgatacgtgc tgcttctgat acattaccca acttttttgc taactctatg gcatcaattt
     2281 tcttctgaat ttctaaatca tacatagaag gttttgtcgg ctcgataacc tcggaaacaa
     2341 cgaggtgacg gccagcaaaa taggcgataa agctgccatt aggctgcttt ctaatttcta
     2401 tttcttgctt ggcaatagag tgttttaggg gtgattctat taggtaaaac ttgttgccat
     2461 agctgaaagt atgatcatta cggattttac gatagtcctt ctgaacacag atatcgtcca
     2521 aatttaaatg tctagggaca gagacgaatt cagacgttac atttttggga tttactgtga
     2581 ggtttttttg ccaaaactca ggaataaaaa attcttgtag ataaacgttg gcactttgca
     2641 tatcgctgat attttgtaag cgtaattctg ggatgaggcg atcttggaat gtatcgaatg
     2701 agcgttcaat acgacccttt ccctgtgcag aattggcaaa tatgatttcg atgccaagtt
     2761 cttcacaggc tctttgcatt tgcgagaagt tacatcgttt cggtcctccg aatatgcctg
     2821 ctctatccac atacagagtt ttgaaaatcc ctttcttttc aatatagtca cgcaggactt
     2881 tcaaacagcc ttgtgtggtt tccgacttaa aaaactcagc atggatttca ctagttgcat
     2941 catcaataat ggcaattaaa caggattttt tatcgccaaa ccactgatgt gagctaccat
     3001 ccatttgcaa taataatcca gaagattcca ttcgttctcg ttttttacgc acctgggagc
     3061 gtctgcgctt agctcgtttt acatgatgaa tatcatgtgc ccaagtacgc aatgtctctc
     3121 gtttaatata tatgttttca ttaaggttta ataattcagc aagatgttgc aaattcacgt
     3181 cataatattt ttctttaatg agggactgga ctttcaattt caaattatca gaggtcttgt
     3241 tgaccggtgg tctaccggta ttgccgtgaa tagcaaactt aataccgaat ttctggtatc
     3301 tattgagata gcgttcgatt gttcgtcgag aacaattgag tagcttagct gcgttattaa
     3361 tggttatctt accttccgat actttggtaa taacatcaac cgtgagctgg gttttggaat
     3421 ccataataat catccttagc aacctctaaa aacactttta agtgtcttca gaaaatcact
     3481 gattaattag atccgtcaat ttcccttggt aattaagata cgacaaagtc gcttggtgat
     3541 aacaaactta cttgtagtag ttcaaacttg atctgacagt taccggtttt tcaagaagtg
     3601 tctgtcaggt caaatttagt ttataaattt ttccatccag atttgtttat cctctatcaa
     3661 agtttgtatt ggagtacgac cacaacacat tttaccttga tgagttcgtt gattattata
     3721 gtaatccatc cattcgtcca gatctttttg taattcatcg attgattcgt aaatcttctt
     3781 tcgaaatgtt acttgataga actcttgtaa aattgtttta tggaaacgct cacaaattcc
     3841 atttgtttgt ggtgagtttg cttttgtctt cgtatggtcg atattattaa tcgccaaata
     3901 aagctgataa tcatggtgct ctacctttcc acaatattcc gtgcctctgt cagttaaaat
     3961 tcgcagtata ggcaagttgt attgctcaaa gaatggtaag actttgtcat tgagtatatc
     4021 tgctgaggta atcggtattt ttgtcgtata aagcttggca aaagctactt tgctgtaagt
     4081 atcaacaaag gtttgctgat aaatacgacc gactccttta agcgtacctg cataaaaagt
     4141 atcttgagag ccgaggtaac cagggtgggc tgtttcaatt tcaccacatg cttcatcatc
     4201 aaatttcttt ttctccaaag cgctaacttg cgcctctgtt aatataatcc cttcagaggc
     4261 tactttggcc tctaatgctt ttaaacggtc tttaaaattg gcaagttcat agcgcaacca
     4321 aatactacga acaccactag gagaaacaaa aataccttgt ttacgaagtt cgttgctcgt
     4381 gcgctgctga ccatgtgctg gaaatgctat cgcgtattcc ttgactgcta gctcgactga
     4441 ttcatctact ctatttttga tattaggttt tcttcggttt tgatcgaata aagcatcaat
     4501 gcctcccgat tcaaccgccg acttatagcg ataaaaagta tctctggata agcccattac
     4561 tttacatgct ttagaaacat ttcctaattc ttcagcaagg tttaataagc caactttatg
     4621 tttaatgatt ttaactgtat tatctatcat gagagttttc ctcttggttt tgtttaaagt
     4681 tcgcacttct atcaaaaccg gaaactctca ctttttcaag tagttgtgtc agattaagtc
     4741 gaaactaatt caacttactc
//
LOCUS       DBNCWK010000177         4606 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.178, whole genome shotgun sequence.
ACCESSION   DBNCWK010000177 DBNCWK010000000
VERSION     DBNCWK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.178"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..1012)
                     /locus_tag="AC06IO_002694"
     CDS             complement(<1..1012)
                     /locus_tag="AC06IO_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HIO9875858.1"
                     /translation="MNKYGGAIILLSFPHQLIHALAALFTDREQRRINKDAPIMIFIW
                     SYRWFDHKKNSPFLEIIKKLSKIENCQLYIPSLLQRVFGLSEFRALKQRVKYVNKIFP
                     LKDYDSFYYSHDFSADHTAQVFMQAFPNIYKVCFGDPPGFLYKNYSSVTECTEKIRSD
                     LKGLFWKSRLKNIHKWYGYDKAYVAMNLNVDAQNYSSILSTIPNERFIELLNKLKSKL
                     SNLEKEEHAFIETLESYNQNKIFQLLVLSNFTESGLITRENELQLYLELCHRHCSPGS
                     IIIIKPHILSNSAFLEALKNNLGKYRVIPLPESLRCIPLEMLTGLIYKCNIVSVSSSS
                     IFL"
     gene            complement(1018..2442)
                     /locus_tag="AC06IO_002695"
     CDS             complement(1018..2442)
                     /locus_tag="AC06IO_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HIO9875859.1"
                     /translation="MSWSIRIENLSKQYRIGSTTTTAAELINEKLRKFLLLLDFKKRN
                     RFSSDKPLPSSIAQEGRTVVDASQISPEYPNHFWALRDINLEIEAGERLGIVGQNGCG
                     KSTLLKILSRIVSPTEGLFRFRGRLISLLEIGTGFHGELTGRENIFLNGTIMGMKTHE
                     IKNRLNQIIEFSELGSMIDTPVKRYSSGMYIRLAFAVAAHLESEILIVDEVLAVGDAA
                     FQRKCTEKMLDLANQGRTLLFVSHDMETVNRICNRAIRMSHGRIVGDSKFDTSAASKS
                     SVMDITRDYLRSGAKYKSEQVWPSDKPMIFLNSLKLNRVRLIDYDNKVRSNFNLNETI
                     VVEIDFTIIKETYPFNIHLYLKDSSGRVIFVSMDNHAWQSKKVRPLGNYTERCTLYSP
                     LLNIGDYHIDIEFWPGNALENRLVVNSVVSFELKDGAVNEGVRGNWASDWPNSLIRPQ
                     LDWSISTNKKQHLVLKEHELVTEK"
     gene            complement(2439..3290)
                     /locus_tag="AC06IO_002696"
     CDS             complement(2439..3290)
                     /locus_tag="AC06IO_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9875860.1"
                     /translation="MTSISSKTQTFKETVVYGVDKFRLLAVLRNIWDYRLLLWIFCKR
                     DLKGRYSQTILGLGWVILTPLITVGVFVIVFGIMIKVPTDGLPTVMFYLVAVIPWYSF
                     LNVLNPSIQMIEGNASLITKVYFPRALIGGAYAMGAAVDFLMAYVFLITPFAIYYGLW
                     SIKLLIIMPFLLISTLMIGLGIGLVLAPINAKYRDIKHFMPLALQLFYYSTPAIYPVS
                     AVPVWAKPWYAVNPLSLVITSYREVLMGHWPSPTIIMTLSGMAIITFLVGLVAFHHME
                     HKVVDIL"
     gene            complement(3256..>4606)
                     /locus_tag="AC06IO_002697"
     CDS             complement(3256..>4606)
                     /locus_tag="AC06IO_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875861.1"
                     /translation="KRRSSFLFYPILFLIGFLCIFIVAIKIFSKILKRIFSNISKFFN
                     ISKFFGGLLKNNSLFQITSGVLKNFGYAYRVLKLHHYDLLIFGQEFPGSINSLLIKLC
                     NKRNTPTLIIPFALGTTKEIVESLYDKDIFDVSSSITNYLASLLYPNWINFYKGKKML
                     RLTGKQIFFLELMGLAPEHPWLPNNSFVTKIAVESEKMFSYYQSMKFPVHQLSLTGSL
                     SDDILVEATTNGQEIKKKICQKMNLDPEKPILLCAWPTDQFGSRFVSMEFKNYEQLCR
                     AWAKCLKEIKREGKYSVIIRPHPVTNKEILGKILGQCDLKQFISYDDTIQLITCCDLF
                     VACVSSTLRWAIAKGIPAINYDCYQYGYTDFDAKGVFNVNDFKSFSAVLEELTRDRQK
                     YEGAKQDQQECASEWGILDGNSQKRIVNLINKLVQSESVIDIENKNYDLNILKNSDI"
BASE COUNT         1570 a          866 c          716 g         1454 t
ORIGIN      
        1 acaaaaaaat agaggatgag gagacagaaa caatattaca tttataaatc agtccagtta
       61 gcatttccaa cggaatacat cgcaaacttt caggcaaagg aatcactcga tatttaccaa
      121 ggttattttt caatgcctca aggaaagcag agtttgataa tatatgaggc tttataataa
      181 taatagatcc tggggaacag tgacggtgac atagttcaag atataattga agttcattct
      241 ctcttgtaat cagtccactt tcagtaaaat tgctaagtac aagcagttga aatattttat
      301 tttgattata agattctaaa gtttcaataa aagcatgctc ctctttttca agattagaga
      361 gcttactttt taatttatta agcaattcaa taaacctttc gtttggaata gtcgataaga
      421 ttgaggaata gttttgagca tcaacattta aattcattgc tacataagct ttatcatatc
      481 cataccattt atgtatattt ttcaaccgac ttttccagaa aagaccttta agatctgatc
      541 taattttttc cgtacattct gttactgagc tataattttt ataaagaaag cctggtggat
      601 caccaaaaca aaccttataa atattaggga aagcctgcat gaatacttgt gcggtatgat
      661 cggcactaaa gtcatgtgaa taataaaagc tatcataatc ctttaatgga aatattttat
      721 taacgtattt tactcgttgc tttaatgccc gaaactcaga taatccaaac actctttgaa
      781 gcaaactggg aatataaagc tggcaatttt ctattttcga taatttctta attatctcaa
      841 gaaatggtga attcttttta tgatcaaacc aacggtatga ccagataaat atcataattg
      901 gagcatcttt attaattctg cgctgctctc tatcagtaaa taatgctgca agagcatgaa
      961 ttaattgatg gggaaaagag agtaaaatga ttgcaccacc atatttgttc atgttgatta
     1021 cttttcagtg actaattcat gctcttttaa aacaagatgc tgtttcttat ttgtcgaaat
     1081 actccaatcc aactgtggtc taatcaatga gtttggccaa tcagaagccc aattgcctct
     1141 taccccctca tttacagctc catctttcaa ctcgaagctg actactgaat tgactaccaa
     1201 tcgattttcc aaagcattac ctggccaaaa ttcaatatca atatgataat cgccaatgtt
     1261 taataagggt gaatacaaag tacatcgttc ggtataattt cccaatggac gtactttctt
     1321 agattgccag gcatgattat ccattgacac aaatatcaca cgcccagagc tgtctttaag
     1381 ataaagatga atattaaaag ggtatgtttc tttaataatt gtaaaatcta tttcaacaac
     1441 aatagtttca tttaaattaa aattagaacg aactttattg tcgtaatcta tcaatctaac
     1501 tcggttaagc ttaagggaat ttaaaaatat cattggctta tcactaggcc aaacctgttc
     1561 cgatttatat ttcgctcctg agcggagata gtcgcgagtg atatccatga ctgaactttt
     1621 gcttgctgca cttgtatcaa atttactgtc accgacaatt ctgccatgcg acattcgtat
     1681 ggctctatta caaattcgat tcacggtttc catatcgtga ctgacaaata ataaagttct
     1741 tccctgattt gctaaatcca gcattttttc cgtacacttg cgttgaaatg ctgcatcacc
     1801 taccgctaac acttcatcaa ctataaggat ttcagactcc aaatgagcag ctacagcaaa
     1861 tgccaagcgt atatacattc cggaagagta gcgcttgaca ggagtatcta tcattgaccc
     1921 caattcagaa aattcaataa tttgattgag tcgattttta atttcatggg ttttcattcc
     1981 cataatagtg ccatttaaaa aaatattttc acgtccagtc agttcaccat gaaatcctgt
     2041 accgatttcc aacaaactga ttaagcgccc cctaaatcgg aataaccctt cggtaggaga
     2101 aacaatgcgt gatagaattt ttaataatgt gctcttaccg caaccatttt gtcctactat
     2161 accaagccgc tcccctgcct cgatttcaag attaatatcc cgcaaagccc agaaatgatt
     2221 gggatattcc gggctaattt gacttgcatc gactacagtt ctaccttctt gtgcaatgga
     2281 tgaaggcagt ggcttatcgg atgaaaatcg attccttttt ttaaaatcca gtaataaaag
     2341 aaattttctc aatttttcat taatcagctc tgctgcggtt gtagtagtac taccaatccg
     2401 atattgtttg gaaaggtttt caatacgaat agaccaactc ataaaatatc aactacctta
     2461 tgctccatgt gatgaaatgc aacaagcccc acaaggaaag ttataattgc cataccagat
     2521 aatgtcatta ttatagtagg tgatggccaa tgccccatta acacctctcg ataactggtg
     2581 ataacaagag ataatggatt aacagcatac catggtttag cccacacagg cacagcagaa
     2641 acagggtaaa tcgctggagt ggagtaataa aataactgca aagccagagg cataaaatgt
     2701 ttaatatccc tatattttgc attgataggt gctaaaacca aaccaatacc taatccaatc
     2761 attaaggtag atatcagcag gaatggcata ataattaata acttgataga ccaaaggcca
     2821 taataaattg caaagggggt tatcaaaaac acataagcca tcagaaaatc aaccgcagct
     2881 cccatagcat aggcaccgcc tatcaacgct cttggaaaat agacctttgt tattaacgaa
     2941 gcatttccct caatcatttg tatggaagga ttgagaacat ttaaaaagga ataccaaggg
     3001 ataaccgcaa ccaaatagaa catgacagta ggcagcccat cggtaggtac ctttatcatg
     3061 atgccaaaaa caatgacaaa caccccaact gtaatcagag gagttaaaat aacccaaccc
     3121 aatcccaaga tggtttgact gtaacgccct ttgagatctc gtttacaaaa aatccataaa
     3181 agcaagcggt aatcccatat atttcttaaa acagctaaaa gcctaaactt atcaactcca
     3241 tagactactg tttctttaaa tgtctgagtt tttgaggata ttgaggtcat aatttttgtt
     3301 ttctatatca attacagatt ccgattgaac taacttatta attaggttca ctattctttt
     3361 ttgggagttt ccatccagga tgccccattc actcgcacat tcttgttggt cctgtttggc
     3421 gccctcatat ttctgcctat cacgcgttaa ttcttccaaa acagcagaaa agcttttaaa
     3481 gtcattcaca ttaaaaacac cttttgcatc aaagtctgta tatccatatt gataacaatc
     3541 ataattaatg gcagggattc cttttgcaat tgcccaacgt aaagttgatg aaacgcaagc
     3601 tacaaataaa tcacaacatg taataagctg tattgtatca tcataggata taaattgttt
     3661 taaatcacac tgtcctaata ttttacctaa aatttcttta ttagtaaccg ggtgaggtct
     3721 aataattaca gaatattttc cttccctttt aatttctttc aaacattttg cccatgctct
     3781 acaaagctgt tcgtaatttt tgaattccat ggaaacaaat cgtgaaccaa attgatctgt
     3841 tggccaagca cacaataaaa taggtttttc cggatcaagg ttcatttttt gacatatttt
     3901 tttcttaatt tcctgaccgt ttgttgttgc ctcaaccaaa atatcatcag ataaacttcc
     3961 tgtcagagat aattgatgaa cagggaattt cattgattgg taatatgaaa acattttttc
     4021 gctttcaaca gcaattttgg taacaaagga attattggga agccaaggat gttctggagc
     4081 taaccccatc agttctaaaa agaaaatttg tttaccagtt aacctgagca tcttttttcc
     4141 tttataaaaa ttaatccaat taggatagag taaagatgca agatagttag ttatacttga
     4201 agatacatca aaaatatcct tgtcatataa gctctccact atttccttag ttgtacctag
     4261 cgcaaaaggt atgattaatg taggagtatt tcgtttatta catagtttaa ttagcaagga
     4321 atttatcgag ccaggaaact cttgaccaaa aatcaaaaga tcgtagtggt gcaacttaag
     4381 aactctataa gcataaccaa agttttttag tacgccactt gtaatttgaa ataaagaatt
     4441 attttttaat aatcctccaa agaattttga aatattaaag aattttgaaa tattactgaa
     4501 aatccttttt aaaatcttgc taaaaatctt aatggcaaca ataaaaatac aaagaaaacc
     4561 aattaaaaac aaaattggat aaaataaaaa agacgatctt cttttt
//
LOCUS       DBNCWK010000178         4548 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.179, whole genome shotgun sequence.
ACCESSION   DBNCWK010000178 DBNCWK010000000
VERSION     DBNCWK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.179"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            295..1971
                     /gene="kaiC"
                     /locus_tag="AC06IO_002698"
     CDS             295..1971
                     /gene="kaiC"
                     /locus_tag="AC06IO_002698"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HIO9875862.1"
                     /translation="MHLLRGRNVNTNKAKTGIKGLDDILNGGYLKNKPTLLKGSPGTG
                     KTIFTLFFTHEQLKSNNSVIYVTCDESPEQIIAHMDGFNLESSKFLQEGKLLILDFTP
                     ILTDEVAGEFNINALLLRIEQAQQKIKADTLIIDSLQSILLGIKNYDPNYELLRLFQW
                     ARQKNLTVLTTMAETQTILQTEIYDEYVVDCAIELKQKVNNDLMTRYLRIIKNRGSSH
                     GTNEYPFSITHNGISLLPITSTKLNTTTSAEYLSTGIKALDSMLGSKGYQTGSQIIIS
                     GRSGTAKTLFATSFAQSAVRQGRKVLFISFEESPNDLIRHVKSINIDLMKYITKKKLH
                     INSRRSVEMGLEDHIISIIELTEEKQFDVLILDPISAMLDLGNTMEVKRLFIRFISYM
                     KSLNKTLLFTELIPDYSKELTILGLSSLTETWIRLSNVESNGEFNRLIHIAKARGIKT
                     SNQIKEFYVTDKGINIEAPYLGDNQMMFGSQKSAHILREKQELQQRKEEIKRIELEID
                     KIAETQRAQLKIQEATFLAKKTELIAKKNELIAKEEALIKRMESNKLLRE"
     gene            1976..2248
                     /locus_tag="AC06IO_002699"
     CDS             1976..2248
                     /locus_tag="AC06IO_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HIO9875863.1"
                     /translation="MTRTKLKLFVIGNSAISKRAIINLQSICSDPKLADLCDIEVVDL
                     CKNKGIAEQEKILATPILIKKEPLPERRIIGDLSDKQKVISALEMD"
     gene            2257..>4548
                     /locus_tag="AC06IO_002700"
     CDS             2257..>4548
                     /locus_tag="AC06IO_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIO9875864.1"
                     /translation="MISNEHINTQNFWYQLVEKMAIGVLVINNEGQILYANDYVVQMF
                     GVPHTELVGTNFLYPMAANETQEVEILKPSNEVLTAHMTVRKSIWENRQVWIISLFDI
                     TELKLKEKLLKISSKGISSAFEGIIITDPEGTIIETNNAFMKMTGYTEQEVIGQKPAF
                     LHSRKQNEKFYKELWNILIAKGQWSGELWAKKKNKRLFPAFLAISAVKNSDGDIINYI
                     GYYHDLTLIKKQEQQIENFKYYDYLTGLPNKFLLTQRLQTLISRLSEVNKELIVFNIR
                     VFDSNSKSSLLDLPPNTRDKIILEAVNRIDRTTPKKMFFSRIGLSEFIVIYFIKNKIE
                     VMSSIAQRMIEKLSYPYYVHHQTIILQTIIGLTAYYKNSSFSAEDLIQQSEIARHKAH
                     IRGVNIFEFFDPQVEERIIEFNKRVESVSKAMQLHELKVYYQPIVELNTAKVVGLEAL
                     LRWQHPEKGLLLPKQFLSDLNNHPLSLQIGDWVLHQVLHQVEELLACNIKIPINVNVS
                     AYQLQHPNFVKKLFDTLARYPNVPHRLLMLEILETEAIEDLTLVSQIIAECKAKGINF
                     SLDDFGTGYASLTFLKELSTSEVKIDRSFIINIFDNPKDLAILKIIIESCKVIGRTLI
                     AEGVETVAHGKLLELLGCHLIQGYAIAKPIPPAKLITWIKNWHLGPEWRETRFSQAEL
                     SNLVKISIKHYLHYQSIRLYLDNHQFPKPQLCLQACPIEHWLLENQQIFHKGKQFKTI
                     SELHHKQHQLGDKIIQLIEAGQKE"
BASE COUNT         1616 a          842 c          790 g         1300 t
ORIGIN      
        1 cttttagctt aaatgaaata accaaaccac atgtcaattg ggatggttga ttcgctggtt
       61 tactttccat tccacaaaat agtagatcga tgaattaaaa gcaaaaactt gtgttcttta
      121 tttaaacgat tcaaccccaa acctattcta gaatgattcg gttagttcta aatacaacta
      181 aatgcaaaat ttattacgac aaaatttgtt acgactctat caagacgaaa tattagaact
      241 ataattaaac aatcagaata ttatttatcg cttcaacagt ggtattttac agatatgcat
      301 ctgttgagag gacgaaatgt gaataccaat aaagcaaaaa cgggaattaa gggattagat
      361 gatattttaa atggtggtta tttaaaaaat aaaccaaccc ttctcaaagg cagtcctggt
      421 acaggaaaaa caatatttac cctttttttt acgcatgaac aacttaagtc aaacaacagc
      481 gtcatttatg tcacctgcga tgagtcacct gagcaaatta ttgctcatat ggatggcttt
      541 aacctggaaa gcagcaaatt tttacaagaa ggcaagcttt taattcttga cttcacacct
      601 atattaacgg acgaagtggc cggggaattc aatatcaatg cacttttact ccgtatagaa
      661 caggctcaac agaaaatcaa agccgataca ctaattattg attcacttca gtccattctt
      721 ttaggcataa aaaattatga tcccaactac gagttgttaa gattatttca gtgggcgcgg
      781 caaaaaaatc ttaccgttct cacaacgatg gctgaaaccc aaacgatttt acaaacagaa
      841 atctatgacg aatatgttgt tgattgtgct attgagctaa aacaaaaagt caataacgac
      901 ctgatgacgc gctatttgcg aatcattaaa aatcgtggtt cgtctcatgg cactaatgag
      961 tatccctttt ctattactca taatggcatt tcattacttc ccatcactag cacaaaactg
     1021 aatacaacaa cttctgctga atatctcagc actggtataa aagccctgga tagtatgcta
     1081 ggcagtaaag gataccaaac aggatcgcag ataataatca gcggacgttc aggaacagca
     1141 aaaacgcttt ttgcaacaag tttcgcccaa tccgctgttc gacaaggcag gaaagtgctg
     1201 ttcatctcat ttgaagaatc ccctaatgat ttaatccgtc atgtcaagtc cattaatatt
     1261 gatttaatga aatatatcac caaaaaaaaa ttacatatta attcaagacg ctctgtggaa
     1321 atgggactgg aagaccatat tatttcaatc atcgaattaa cagaagaaaa gcaatttgat
     1381 gtccttatac tggatcccat ctcagctatg ctggatttag gtaatacgat ggaagtcaag
     1441 agactgttca tacgttttat ttcttatatg aaatctctca ataaaaccct tttatttact
     1501 gaactgatac ccgattattc caaagagcta accatactgg gcttatccag cttaacagaa
     1561 acctggattc gcttatctaa tgtggaaagt aatggagaat tcaatcgatt aatccacatt
     1621 gccaaggcgc gtggcataaa aacatccaac caaatcaaag aattttatgt cactgataaa
     1681 gggattaaca tagaagcccc ttatcttggt gataaccaga tgatgtttgg ttctcaaaaa
     1741 tcagcccaca tcttgagaga aaaacaagaa ttacaacaga ggaaagaaga aattaaacgg
     1801 attgagcttg aaattgataa gattgcagaa acacaacgtg ctcaactcaa gattcaagag
     1861 gcaacctttt tagctaaaaa gactgaattg attgctaaaa aaaatgaatt gattgctaaa
     1921 gaagaagcgc ttattaaacg catggagtcc aacaaattgt taagagagta aatgaatgac
     1981 tcgaactaag ctaaaactgt ttgtgattgg aaatagtgct atatcaaaac gtgccattat
     2041 taatttgcaa tcgatatgct cagatcccaa gcttgctgat ttgtgcgata tcgaggtagt
     2101 cgatctatgt aaaaacaagg ggatagcaga acaagaaaaa attctggcaa ctccgatatt
     2161 aattaaaaaa gaaccattac ctgaacgtcg aatcatcggt gatttatccg ataagcaaaa
     2221 agttatttct gctttggaaa tggactaggt gatatcgtga tatcaaacga gcatataaac
     2281 acacaaaact tttggtacca actcgttgaa aaaatggcta ttggggtatt ggtgattaat
     2341 aatgaagggc aaattcttta tgcaaatgat tatgtagtac aaatgtttgg tgtaccccat
     2401 acagaacttg ttggcaccaa ttttttatat ccgatggcag caaatgaaac acaggaagtt
     2461 gaaattttaa aacccagtaa tgaagttttg acggcacata tgacagtcag gaaaagtatc
     2521 tgggaaaatc gccaggtatg gattatttct ctctttgata ttacagagct caaactaaaa
     2581 gaaaaacttt taaaaatttc aagcaaaggc atcagttcag cgtttgaagg tattatcatc
     2641 accgatccgg aaggcaccat tattgaaacc aataacgcat ttatgaaaat gacaggatat
     2701 acagagcagg aagtcatagg ccaaaaacct gctttcttgc attcaaggaa acaaaacgag
     2761 aaattttaca aggagctatg gaatatactc atagcgaaag gtcagtggag cggtgagctt
     2821 tgggcaaaaa agaaaaataa aaggttattt cctgcctttt tggccatctc ggccgttaaa
     2881 aattccgatg gtgatatcat caactacatt ggctattatc atgatctcac gttaataaaa
     2941 aaacaagaac aacaaattga gaatttcaaa tattatgatt atttaacagg attaccaaat
     3001 aaatttttac tcactcaaag acttcagacg cttatcagca gattgtccga agtaaataag
     3061 gagctcatcg tttttaatat tagagtattt gactccaata gcaaaagctc tctgttagat
     3121 ttacccccca atactcgcga taaaatcata ttggaagcag ttaaccggat tgatcgaaca
     3181 acgccaaaaa aaatgttttt ctcacgcatc ggtttaagtg agtttattgt tatttatttt
     3241 atcaaaaaca aaattgaagt gatgtcttcg attgcacaaa gaatgataga aaaactctct
     3301 tatccttatt acgttcatca ccaaactatt attttgcaaa cgatcattgg attaaccgcc
     3361 tattataaaa atagctcatt cagcgccgag gatttgatac aacaatctga aattgcccgc
     3421 cacaaagcgc atatcagggg tgttaatatt tttgaatttt ttgaccctca ggttgaagag
     3481 cgaattatag aatttaataa gcgcgttgaa tctgtctcta aagcaatgca gttacatgaa
     3541 ctgaaagttt attaccaacc tattgtagaa ttaaatacgg ctaaagtggt gggtttggaa
     3601 gcgctacttc gttggcagca tccagagaaa ggtttattgc ttcccaaaca atttttatcc
     3661 gacctcaata atcatcctct ttctctacag attggtgatt gggtgcttca tcaggtatta
     3721 catcaagttg aagaattgct cgcttgcaat ataaaaatcc ccatcaatgt taatgttagt
     3781 gcatatcaac tgcaacatcc caactttgtc aaaaaactgt ttgacacatt ggcacgatac
     3841 cctaatgttc ctcatcgcct gcttatgctt gaaatccttg aaacagaagc tattgaagat
     3901 ttgaccctag tatcccagat aatagccgag tgcaaggcta aaggaatcaa cttttccttg
     3961 gatgactttg gaacaggtta tgcttcttta actttcctaa aagaattgag tacctcggaa
     4021 gtgaaaatcg atcgaagttt tatcattaac atctttgata accctaaaga tctcgctatt
     4081 ttgaaaataa ttatagagtc ttgcaaagtg attggccgca ccctgattgc tgaaggagtt
     4141 gaaacagtag cacatgggaa actattagaa ttacttggtt gccatctaat ccagggatac
     4201 gccattgcca aacctatccc tcctgcaaaa ttaattacat ggataaaaaa ctggcatctt
     4261 ggcccagaat ggagagaaac cagattttca caagcagaac tatctaatct ggtaaaaatc
     4321 tccataaaac actatctaca ttaccaatca atcaggctat atttagacaa tcaccaattt
     4381 ccaaaacccc aattatgcct gcaagcatgc ccaattgagc attggcttct tgagaaccag
     4441 caaatctttc ataaaggcaa acaatttaaa acaatctctg agttgcatca taaacaacac
     4501 caacttgggg ataaaataat tcaattaatt gaagctggac aaaaagaa
//
LOCUS       DBNCWK010000179         4342 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.180, whole genome shotgun sequence.
ACCESSION   DBNCWK010000179 DBNCWK010000000
VERSION     DBNCWK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.180"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..225)
                     /locus_tag="AC06IO_002701"
     CDS             complement(<1..225)
                     /locus_tag="AC06IO_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875865.1"
                     /translation="MPLSVSDCQSNFPVMNNGHHLHHQKQAGIEKSKITQTAAQTANN
                     TNSAIVTIEPEYDVDSEKKVTQVGEARRFFI"
     gene            468..3425
                     /locus_tag="AC06IO_002702"
     CDS             468..3425
                     /locus_tag="AC06IO_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIO9875866.1"
                     /translation="MNTHNAVLICFLELGYSDVEGVCHGISIRWLEACMSGEEHVFHE
                     RIKRIEQIVSSGQDIVKLMDAVKEKKGKNLSAEDQDLLDIQAFFDSLDLYHMPYRHTE
                     LFGLSSDSNQKNIAFHSDLAASDKINALGGLSQVYSQALIPDEAELKHYLDELDFILQ
                     AGSGSSKDVIGVVLSSFNHTLAVTYKPGEGWGFMDINQYPSKVFKTEETGLLAKRIIK
                     GLNSVPPSSYLALDASIYTTTRNPQVYSLTTQLGQFKSSHVMTQEIASREQHGMGLAW
                     IAARIGDIGLISELAKHQVDLNKADKDGFTPAHLAVLHGDVTFISELARHQVDFNKVT
                     KHGVTTAHLAAQQGDVAVIAELARNGVNFNKTSRSGNTPAHLAAQNGHVAAIIELAKY
                     GVDFNKTNERGDTPAHLAARNGHVAVIAELAKHGVNFNKANRSGDTPAHLAAQNGHAA
                     VITELAKNNVNVHQQNEHGSTLAHIAALNGHAVVIAEVAKHQVDFNKKDINGSTPAHL
                     AARNGHVAVIAELAKHQADLNKADNDGFTPAHIAALTGQSMVLDELIKSGADLSPSLI
                     SLTNWKDKAYLKQDAVSRMNSFLNYHKDKHEVLLSPRELAVIMGDENEIQRFLDNVVE
                     PHLRYAGRADLAEKDKDYEPLPKEVIACLNQLIDLKRQNEILESYLAETETLLNAIKE
                     NEQRIIDRKYYIDFIDEHKESYTNVMWHITSPAKEELFQNEAQLRHSGLSSTVLYGMS
                     WATSLFSTCYRFLTPKLQQEYLDSLMPQTLDSQSKALFKRLLQEHKDNLKKEMARSEQ
                     FITNINKQLFPESEEMLPLFSTLSSKNLQLLPIYEFRIMKSRLAEDSDVPKKPIDAQS
                     LMHKKSSMSILRIPATEREENIYAVFQMLECPLMCNEDGSIPKILKEIKAIIKEIDPQ
                     EEQSISDALRLIRNVVKDDAGQERPLVAEVLKIFDATGSLNFRQIRNSLEHLPGLNEF
                     SLSLRRPLSGHST"
     gene            complement(3458..3946)
                     /locus_tag="AC06IO_002703"
     CDS             complement(3458..3946)
                     /locus_tag="AC06IO_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIO9875867.1"
                     /translation="MKIRAIFFMIAIFFPVTLFASNTTCPLSNGINVLTKKRILNICK
                     NGTVVKTFKVALGYKGVGKKKAGDNKTPIGLYGLAHPRKSNQFKVFIPILYPTKQQLA
                     AGYTGRDVGIHGPTQSPGVFGWLNNLPGSTRGCVAVGKNNYIEYVANWVKANPGAKVL
                     II"
BASE COUNT         1392 a          797 c          881 g         1272 t
ORIGIN      
        1 tataaaaaat cgtcttgcct ccccgacttg agttactttc ttttcactat cgacatcata
       61 ttcaggttca atggttacta ttgcagagtt tgtattatta gctgtttgag cagcagtttg
      121 tgttattttg gatttctcga tcccggcttg cttctggtga tgtaaatggt gcccgttgtt
      181 catcaccggg aaattcgatt gacagtctga tacagataaa ggcatatgaa ttccaaacta
      241 agtatattta catttaaata aaagtataga acaaatacca agagttttta ttaccaaaaa
      301 aattgatatc tgggttaagt caaacacatt cataaagacc agctttatta gatcaaactt
      361 tctatttgat ctgatgagat aggaggggct tgcactctac atcatggttc gaagcataat
      421 acctgtgtct ataattataa ctacaactaa tcatttgatt tcctaaaatg aatacacata
      481 atgcggtatt aatttgcttt ttagaattag ggtattccga tgttgagggc gtatgtcatg
      541 gaatttcaat ccgctggctg gaggcctgca tgtcaggtga agagcatgtg tttcatgaac
      601 ggattaaacg cattgagcag attgtttctt caggtcaaga tatagtgaag cttatggacg
      661 cggttaaaga aaaaaaaggc aaaaacctat cagccgaaga tcaagactta ttagatatcc
      721 aggctttttt tgacagcctg gatttatatc atatgcctta tcgccataca gagctatttg
      781 gcctttcatc ggatagtaac caaaagaata ttgcatttca ttctgatctg gcagcatcag
      841 ataaaatcaa cgcattggga ggtttaagcc aggtatattc ccaagcactc attcctgatg
      901 aagcagaatt aaagcattat ttggatgaat tagattttat cttgcaagcg ggttctggct
      961 caagcaaaga cgtgatagga gttgttttaa gctctttcaa ccatactctg gcggtgactt
     1021 ataaacccgg tgagggttgg gggtttatgg atattaatca atacccatct aaggttttta
     1081 aaaccgaaga gacaggatta ctcgctaaaa ggataataaa gggtttgaat tcagtcccac
     1141 catcttcata tcttgctttg gatgcatcga tttacaccac aaccagaaat ccacaagtgt
     1201 attcattaac cacacaattg ggtcaattta aatccagtca tgtaatgacc caagaaatag
     1261 cttcccgaga acaacatggg atgggtttag cctggattgc agcacgaata ggggatattg
     1321 gacttatctc ggagcttgcg aagcatcaag ttgatttgaa taaagcggat aaggatggct
     1381 ttacgccagc tcatttagct gtcctgcatg gtgatgtaac ttttatctca gagcttgcga
     1441 gacatcaagt tgatttcaat aaggtgacta aacatggcgt tacaacagcc catttagccg
     1501 cccaacaggg tgatgttgct gtcattgctg agcttgcaag aaatggagtt aatttcaata
     1561 aaacaagtag aagtggaaat acgcctgctc atttagccgc tcaaaatggt catgttgctg
     1621 ctattattga acttgcaaag tatggggttg acttcaataa aaccaatgaa agaggtgata
     1681 ctcccgctca tttggcagct cgaaatgggc atgtggccgt cattgctgag cttgcaaagc
     1741 atggggttaa tttcaataaa gcaaatagaa gtggggatac gccagcccat ttagcagctc
     1801 aaaatggtca tgctgctgtt attaccgagc ttgctaagaa taatgttaat gtacatcaac
     1861 aaaatgaaca tggttctaca ctggcccata tagcagccct aaatggccat gctgtggtca
     1921 ttgcggaggt tgcgaaacat caagttgatt tcaataaaaa agacatcaat ggttctactc
     1981 cggctcattt ggcagccaga aacgggcatg ttgctgttat tgcagagctt gcgaagcatc
     2041 aggctgatct caataaagca gataatgatg gctttacccc ggctcatatc gcagcattaa
     2101 ctggccaatc tatggttctt gatgaattga taaaatcagg tgctgattta agcccttctt
     2161 taatttcact caccaattgg aaagataaag cctacttgaa acaagatgct gtttctcgaa
     2221 tgaattcatt ccttaattat cataaagaca aacatgaggt gttgctttct cccagggagc
     2281 tggctgtaat tatgggggac gaaaatgaga ttcaacgttt cctggacaat gtggttgagc
     2341 ctcatttgag atatgctggc agggctgatt tggcagaaaa agacaaagat tatgagccat
     2401 tacccaagga agtgattgca tgcctgaacc agctaatcga tttaaaacgg cagaatgaaa
     2461 tacttgagtc atatcttgca gagactgaaa cattgctcaa cgcaataaaa gaaaatgaac
     2521 agcgtatcat agaccgaaag tattatattg attttattga tgagcataag gaatcctata
     2581 ccaatgtcat gtggcatata accagtcctg caaaagaaga gttatttcaa aatgaagccc
     2641 aacttcgcca ctcaggactg tcatccacgg tgctttatgg aatgagctgg gctacttcat
     2701 tattttccac ttgctaccgt tttttgactc ccaagctcca acaagaatat cttgactcct
     2761 taatgcctca gactttagac agccaaagta aagctttgtt taaaaggctc ttgcaggagc
     2821 ataaggacaa cctaaaaaaa gagatggcca ggtccgagca atttattact aatatcaata
     2881 aacaattatt ccctgagtct gaagagatgc tccctctgtt cagtacgctg tcgtcaaaaa
     2941 acctgcagct actgcctata tacgagtttc gtataatgaa aagcagactt gcagaagaca
     3001 gtgatgtgcc aaaaaagcct attgatgcgc agagcttgat gcataaaaaa tcatcgatga
     3061 gtattctaag aattccagcg acagagcgtg aagaaaatat ttacgcggta tttcaaatgc
     3121 tagagtgtcc tttaatgtgt aatgaagacg gctcaattcc caagatttta aaagaaatca
     3181 aagcgattat aaaagaaata gacccacaag aagagcaatc aattagtgat gcgcttcgcc
     3241 tgattcgcaa tgtggtaaaa gatgacgccg gacaagagag acctttagtc gctgaagttc
     3301 taaaaatatt tgatgctacg ggtagcctta attttagaca aattcgcaat tcgttagagc
     3361 acttacctgg tttaaatgaa ttttctttat cattaagacg accactatca gggcattcta
     3421 cataaaataa ataggcagta tcaaatttcg tctcaattta aataattaaa accttagctc
     3481 caggattggc ttttacccaa tttgctacat actcgatgta gttgtttttg ccaacagcaa
     3541 cacatccacg agttgagccg ggtaagttgt ttaaccaacc aaacacgccg ggcgactggg
     3601 ttggtccatg tattccgaca tctctaccag tatatcctgc agctaattgc tgtttagtcg
     3661 gatagagaat tggaataaag actttaaatt gattggattt ccttggatgg gctaacccat
     3721 ataaaccaat tggagttttg ttatccccag cctttttttt accaacgcct ttgtacccga
     3781 gcgctacttt aaaggtttta acgacggtgc cgtttttaca aatatttaaa attcgctttt
     3841 tagtgagtac attgattcca tttgataaag gacaagtcgt attactggcg aataaagtaa
     3901 ctggaaaaaa aatagcgatc atgaaaaaaa ttgctcttat tttcatataa ataggttaat
     3961 attgctgaag ttgggcaata attatcccac aatattgttt ttattcatag agctgacagg
     4021 ctaaatctta tgtaaatata cgtagcttga aagtctgttt aggaaaaaat caatttctat
     4081 ggcgtgctct gaagaactct ccctcagaaa tcttggttcg aagccaagta ttttagccat
     4141 gacaaacttt aaaataaaga tttgatgaga cggtgattaa tgataactaa gaagatctgg
     4201 aacaatttta aaaaaaactg agaaaatata tcaaaaaact tgaaagatgt atgggcctat
     4261 tgcttatgca atgaggaaag cattttacag ttggcaatag aaataaataa ttcaccaggc
     4321 acaggatact tggagataat gt
//
LOCUS       DBNCWK010000180         4171 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.181, whole genome shotgun sequence.
ACCESSION   DBNCWK010000180 DBNCWK010000000
VERSION     DBNCWK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.181"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            163..1371
                     /locus_tag="AC06IO_002704"
     CDS             163..1371
                     /locus_tag="AC06IO_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875868.1"
                     /translation="MVRHLKLIIILAFTLAGDILFGLALIWRVLALDGSAKTLGLFLC
                     VITLVTFTLQKSSIDFRRMLEKTPRRSFMWIRVVGVGFSIIFIPLLWHETTWLLYLVG
                     AIATIINFLSILTLEAMMAHEVFEKRISSNKASRILQIAILASSFLGASLLGTILALG
                     NIIAVLLFCIATYLVGAICFSTQSDVGIQKISQVQTAKTASNQKMIYGNNLSVLWLAF
                     IGLTLLMAQISAFNFLLPLIAQHEKFWTASQYGFIDAAAELGGFLATIIMFEKGIFSK
                     LWIFAFLGIALVDILFYAVTDIYLISTATSFLGFCVSCFRIKQREYIYDSVSNTDEII
                     EWTGRITAMTAIIKALTPLGLAFFISQPSFDLMIIGIVVGTVMLIVTSVLYFYMNKEF
                     KQQIPSYSAV"
     gene            1406..2698
                     /locus_tag="AC06IO_002705"
     CDS             1406..2698
                     /locus_tag="AC06IO_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIO9875869.1"
                     /translation="MKKCVLIVDPFSSGALYAEALIKRGIVPVAILSHYPIIEVYRDS
                     FKSSDFKYIFTYNENADFYKELNIFLRDKELIVVLAGAESGVELADKLSERYCIDHSN
                     GTALSSCRRNKYEMVQKLGQLHIDVPLTIKSNSTDEFLAWINNNNLFTKGVVIKPLKS
                     AGTDSVHACFNEQELIEAVNQNIGKVNQLNFKNDDLMVQEYLIGTEYVVDSTSINSNH
                     IITNICCYKKTNANNSKFVYDYLDFLPETGDVQHNLAEYTYKVLDGLGIKYGPAHSEI
                     MLTDKGPQLIETGIRPHGGVAIPACRLATGNSHLDLTLDLILSKSKRLENKIGFKLIK
                     HTRIVFLISHFESYILDEHQNLEYIKKLISFVTLKLNVRPGKYLKKTVDLFSMPGLLI
                     LSHESKEQVESDYNLVRKLEQKGLFVLAASQESKTTKI"
     gene            2714..3025
                     /locus_tag="AC06IO_002706"
     CDS             2714..3025
                     /locus_tag="AC06IO_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HIO9875870.1"
                     /translation="MQIIKSSLFTGDFTWQALDIAQISDASIRLHWADQPYIWHKNDG
                     DEVFVVLSGKVIMHYREGGVENQKELNSGDICYLKEGCEHVAYPKGGARILVIEKAGS
                     I"
     gene            3072..3335
                     /locus_tag="AC06IO_002707"
     CDS             3072..3335
                     /locus_tag="AC06IO_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875871.1"
                     /translation="MSDSVGKLKTPKFKKFRGKLGEYLKAPSALPIKHGLELAIKQIK
                     RSDIVVSRNQGQNYGKLFDDAKRRKNYLYTSDLMLFRGIFISI"
     gene            3359..3556
                     /locus_tag="AC06IO_002708"
     CDS             3359..3556
                     /locus_tag="AC06IO_002708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875872.1"
                     /translation="MDENYIAYQLQVDEVGLKEKRSSEGITSNTIKDIKQKIDQTSKN
                     GGWVIFKKNGNQYELCELYKP"
     gene            3641..4077
                     /locus_tag="AC06IO_002709"
                     /pseudo
     CDS             3641..4077
                     /locus_tag="AC06IO_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611658.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1415 a          587 c          808 g         1361 t
ORIGIN      
        1 tggtccatta agcctaggaa atatatgcca agaatcacaa aggatccaat gcccttggca
       61 tgatgggtat tttaagattt cttcattaat aaaaaatgca atcccggctg ttcgtgttaa
      121 ggatcagatt ttctatattt aaatattaaa aggattatta ttatggtgcg tcatttaaaa
      181 cttataatca tattagcatt tactcttgca ggtgacatat tatttgggct ggcgcttatc
      241 tggagagttt tagctttaga tggttcagca aaaacattag gtctttttct ttgtgtaatt
      301 actcttgtaa cttttacatt acaaaaatct agcattgact tcaggaggat gctagaaaaa
      361 acaccacgtc gctcttttat gtggataaga gtagtaggcg ttgggttctc tattatattc
      421 ataccgctat tatggcatga aactacgtgg cttctttatc tagtaggtgc gatagcaaca
      481 attattaatt ttttgagcat cttaaccctt gaagcaatga tggctcatga agtgttcgaa
      541 aaaagaataa gctcgaataa agcctcgcgt attctacaaa tagctatcct tgcctcatct
      601 tttttgggcg ccagtttgct tggaacaata ttagcacttg gtaatattat agctgtttta
      661 cttttttgta ttgcaactta tttagttggc gcaatatgtt tttcaacaca gtcagatgtc
      721 ggcatccaaa aaattagtca agtccagact gcaaaaacag cttctaatca gaaaatgatt
      781 tatggaaata acttatcagt tttatggctg gcatttatcg gtctaacatt gttaatggca
      841 caaatatctg cctttaattt cttgttacct ttaattgctc aacatgagaa attttggacg
      901 gcctctcaat atggatttat tgatgctgca gcagagttag gtgggttctt agctacgatt
      961 attatgtttg aaaaaggaat tttttcaaag ctatggatat ttgcatttct cggaattgca
     1021 ttagtcgata ttttatttta tgcagtgaca gatatttatc ttattagcac agctacatca
     1081 tttcttggat tttgtgtcag ttgttttcgg ataaaacaac gagagtatat ctacgactca
     1141 gtatcaaata ctgatgaaat aatcgaatgg accgggcgca tcactgcaat gacggccatc
     1201 ataaaagctc ttacgccgct tggtttagct ttttttattt ctcaaccctc gtttgattta
     1261 atgataatcg gaattgtggt gggaacggta atgttaatag ttacatccgt attgtatttc
     1321 tatatgaata aagagttcaa acaacaaata ccaagctata gtgcggttta gttaataaat
     1381 taaatttata caaagtgaga ttgatatgaa aaaatgtgtt ttaattgttg accccttttc
     1441 ttcaggtgca ctatacgctg aagcactcat aaaacgaggc attgttcctg ttgctatctt
     1501 atcacattat ccaataattg aagtttatag agattcgttc aaatcttctg attttaaata
     1561 tatttttact tacaatgaaa atgctgactt ctataaagaa ctaaatatat ttttaagaga
     1621 taaagagcta atagtagttt tagctggagc tgagtctgga gttgaattag cagataagct
     1681 gtctgaaaga tattgcattg accattcaaa tgggacagct ttaagcagtt gtagaagaaa
     1741 taaatatgaa atggtacaga aattaggcca acttcatatt gatgttcctt taacaattaa
     1801 aagtaattca actgatgagt tccttgcgtg gattaataat aataatttgt ttactaaagg
     1861 tgttgttatt aaaccattaa aaagtgcagg aactgatagt gtacatgctt gctttaatga
     1921 gcaggagttg attgaggcgg taaaccaaaa tataggcaag gttaatcaac ttaattttaa
     1981 aaatgacgat ttaatggttc aagaatactt aattggcaca gaatatgtag tagattctac
     2041 gagtattaat agtaatcata taataactaa tatttgttgc tataaaaaaa caaatgctaa
     2101 taattcaaaa tttgtttatg attatttaga ttttttacct gaaacaggag atgtacagca
     2161 taatcttgct gaatatacat ataaagtact tgatggttta ggaattaaat acgggccagc
     2221 tcattcagaa attatgctga cagataaagg accacaatta attgaaacag gaattcgacc
     2281 acacggtggt gttgctattc ctgcatgcag attagctacg gggaatagtc atctagactt
     2341 aacattagat ctaattttaa gcaaatctaa acgattggaa aacaaaatcg gatttaaatt
     2401 aattaaacat actcggattg tcttcttaat ttctcatttt gaaagctaca ttttagatga
     2461 acatcaaaat ctagagtata ttaaaaaatt aattagtttt gtaactctta aacttaacgt
     2521 tcgaccaggt aagtatctta agaaaacagt cgatcttttt tccatgcctg gtctattaat
     2581 tctaagccat gaatcaaaag aacaagttga aagtgattat aatttagtta ggaaattgga
     2641 gcaaaaagga ttattcgttc tggctgctag ccaggagtct aaaaccacta aaatttaata
     2701 agcgaaaggt aatatgcaaa taattaaatc atcattattt actggcgatt ttacatggca
     2761 agcattagat attgctcaaa tatctgatgc gagcattaga ctgcattggg cggatcaacc
     2821 atatatttgg cataagaatg atggggatga ggtttttgtt gtgctgtctg gaaaagtgat
     2881 tatgcactac cgtgagggtg gtgtagaaaa tcaaaaagaa ttaaatagtg gagacatatg
     2941 ctatttgaaa gaaggatgtg agcatgtagc ttatccaaaa gggggggcaa gaatacttgt
     3001 catagagaaa gctggtagta tttaaaaatt gatttatgtt ttcaggtaat ccctaaaaag
     3061 gatatttatt gatgagtgac tcggtaggca agttaaaaac tcctaaattt aaaaaattca
     3121 gaggcaagtt aggggaatat ctcaaagcgc cttctgcact accaattaag catggtttag
     3181 aattagccat caagcaaatt aaaagatctg atattgttgt atctaggaat caaggtcaga
     3241 actatggaaa attgtttgat gatgcgaagc gaagaaaaaa ttatctatat acttctgacc
     3301 tcatgttatt taggggaata tttattagca tttagcatac acacattagg agatatggat
     3361 ggacgaaaat tatatagcat atcaacttca ggtggatgaa gtaggtctta aggagaagcg
     3421 ttcaagtgag ggtataacaa gcaacaccat taaggatata aagcaaaaaa tagatcaaac
     3481 gtctaaaaat ggtggctggg tcattttcaa gaaaaatgga aatcaatatg aactatgtga
     3541 actatataag ccttgataat aggaaataac caaggaaagg taatgtaaat aaagtaagag
     3601 gagtcgcttt taatcatttc aaagctaata acagaattga gcaatcgttc tggatttatt
     3661 ttcaaggaag gtggttggtt ggagcatggg atccagaatg aaagcaacat tggtttgtga
     3721 tgctccgaat atggccattt ggcagcaaaa ccttcatcag gacttattgt tcattcagac
     3781 agaggagttc aatatgcgag ctataaatac cgcaatttat taaaaagtaa aggctatatt
     3841 ggcagtatga gtaaaaaagg tgattgctgg gataatagtg ttattgaaag tttctttggc
     3901 agcttgaaga aagaacgagt tcaatggcga aattatcaaa cacgatggga ggctcaacag
     3961 gacattttga attatatcac catgttttat aacagccaca gactacattt ttatttggga
     4021 tatttaagcc caaatcaatt tgaaaaacag agtgattttt taatgaaagt tgcttaactg
     4081 gggtgtaaca ttttggttga ccacgtcaag gttgtatgtt gtagtttttg agaatatcat
     4141 tggtcgcaat ggtttttaga acttacttgt a
//
LOCUS       DBNCWK010000181         4004 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.182, whole genome shotgun sequence.
ACCESSION   DBNCWK010000181 DBNCWK010000000
VERSION     DBNCWK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.182"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1631
                     /locus_tag="AC06IO_002710"
     CDS             <1..1631
                     /locus_tag="AC06IO_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg2527 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875873.1"
                     /translation="RYPMSYQKIELSISLSLDSPKLDESDFILLSVKYLEKSLGKKKE
                     FSGFFEDIEQLYFKQNYKEAIEKILDFSKKNESLLSEQVVQRLAEVAPRLKSNPKDNE
                     SRRLYETLYADHLESVIKQESDLSVFNELRDSYNAVKPEYAVTHESEIKTLDEAKQFI
                     LSFVMLNDNVELPLKAQSERYPKKDRSREELGNTPSANPGIMKPNSPNFTDNLVPVRD
                     VPKIAINEKVAGGYSKTKPTTPFVASLSGTTYSLMVVLTDYIEKHKTDKDIEKKVNQI
                     INLWISSYIKEGYHSYSEVVDVLTEPFLQSIFDKANIKLNYGVLEDTHAEFRKAQDYV
                     FGLTIRSAMHHELQERFKNKEKLQEEVKNFESGLNKLNQNVENTGRHREALNKLNEVF
                     QDWSAGKKSYDAFKGESNQLIYDIESEEQKSNRGLGSMLKNLGNYLLYLITLRFLKED
                     YPKPSSPVTMLVTELRGTLEQIDHFHKTSLSLKKATQPDPKNTSPSGETTDVLKSAVD
                     TDKHDKNQESEVIKTLDSEQLEEERQDDKIARLQ"
     gene            1976..3340
                     /gene="mavL"
                     /locus_tag="AC06IO_002711"
     CDS             1976..3340
                     /gene="mavL"
                     /locus_tag="AC06IO_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavL"
                     /protein_id="HIO9875874.1"
                     /translation="MRLFQYNQHICRGRFPVSNAYCKDFVVRNINTIFGLSENIMAYQ
                     LLLSKETLNKILQYKQNLEKGLATPGKFFLEELSKQEKSISEMDITTFTQLLIQSKKP
                     QVFAESQVYHDGTDWTLEEESILGDVSVNMPVTMYNDGGHGSSFKNHPEPISGYLAYV
                     PGALLASGSGPTSDMKEVLDNGKLNQDKLNALYERRLLPQLIHFNELARQNEKQAAIT
                     IPGIGTGCFSGAYYDVIKPYVRNALIHILEKHKDSLPYIDIIHYDPYMGDEPAEKKIG
                     HMSFRVSPSGVVRGTTGQLDYPLGSNPDTHILVSIVAWDHFSWPGNDYWGGARQTDDG
                     VKAASTDTMGQVTGATGVYDKKWGRYMPPESFTKDRKGMSDWGDYVRENGIVFNGPVL
                     ALDKSGKLDTLENVASRSKAKVDTTTTISELVRGMFSLFSHSTTTEPSPTIKEEESKK
                     SGPQ"
     gene            complement(3565..>4004)
                     /locus_tag="AC06IO_002712"
     CDS             complement(3565..>4004)
                     /locus_tag="AC06IO_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIO9875875.1"
                     /translation="ENLSSRARRLHTKDNFLTLPRECIIQMNELISSGNTSKKDNLKE
                     IILRSSQQKMAELIKDNVFDYNQLPFSFLAAKDLTHREKEMIYLYFNDFNLSRIASIF
                     EISKRTVERHFENIKKKLGCENTGQIIPALIKYDCSLKKGIKT"
BASE COUNT         1313 a          686 c          792 g         1213 t
ORIGIN      
        1 gcagatatcc tatgtcatac caaaaaatag aactcagtat ttcattatca ttagattctc
       61 caaaattaga tgagtctgat tttatattgc tctcagttaa gtatcttgaa aagtcacttg
      121 gcaaaaagaa agagttttct ggtttttttg aagacataga acagctctat tttaagcaga
      181 attacaagga agcaattgaa aaaatattgg atttctctaa aaaaaatgaa tccttgttaa
      241 gtgagcaagt tgtccagagg ctggcagagg tcgctcccag attaaaaagc aatcctaaag
      301 acaatgaaag cagaaggctt tatgaaacct tgtatgcaga tcaccttgaa tcagtcatta
      361 aacaagagag tgatctttcc gtatttaacg aattgagaga cagttataat gcggtgaaac
      421 ctgagtatgc agtaactcat gagagcgaaa tcaaaacttt ggatgaagca aagcaattta
      481 ttttatcttt tgtcatgctg aatgataatg ttgaattacc tttaaaagca caatcagaga
      541 gatacccaaa aaaggacaga tccagagaag aattggggaa tactccatct gcgaatccag
      601 ggattatgaa acctaactca cctaatttta ccgataacct ggtgcctgtc agagatgtgc
      661 ccaaaatagc cattaatgag aaagtggcgg gtgggtattc aaaaaccaag cctactactc
      721 cttttgtggc gtcgctgtct ggcacaacct actcacttat ggtggtgtta acggactata
      781 ttgaaaagca taaaacagat aaagacattg aaaaaaaagt aaaccagatt attaatttat
      841 ggatttccag ttatatcaaa gaaggttatc acagctacag tgaggttgtg gatgttttga
      901 cagagccgtt tcttcaatct atttttgata aagcaaatat taaattaaat tatggtgtac
      961 ttgaagacac gcatgcagaa tttcgtaaag ctcaggatta tgtttttggt ttgacaattc
     1021 gatcagcaat gcatcatgag ttacaagagc gatttaagaa taaagaaaaa ctgcaggaag
     1081 aggtaaagaa ttttgaatct gggttaaata aactcaacca gaatgtcgaa aatacaggtc
     1141 gccatcgcga ggctctgaat aaattaaacg aggtatttca ggattggtct gcgggcaaaa
     1201 aaagctatga tgcttttaaa ggtgagtcta atcaactgat ttatgatatt gagtctgaag
     1261 aacaaaaaag taatcgtggt cttggctcca tgctgaagaa cctcgggaac tatcttttat
     1321 acttgattac ccttcgtttt ttgaaagaag actatccaaa accgtctagt ccggtcacaa
     1381 tgttagtcac tgaattaaga ggcacacttg agcaaataga tcattttcat aaaaccagcc
     1441 tctctttaaa aaaagcaacg cagcctgacc ctaaaaatac ttcgccatct ggtgagacaa
     1501 cagatgttct gaaatcagct gtcgatactg ataagcatga taaaaaccag gaaagtgaag
     1561 taataaagac attagactct gagcaactgg aggaagaaag gcaagatgac aaaatagctc
     1621 gtttacaata gagctgttgc ataacccact cctttcattt tattgccacg ttgcaaatga
     1681 ttctgaactg ctcttatgta aacttcactc ctcgaaacgt ttgcactttg ccttaaacta
     1741 aaataatcag tttgggcaag aaggctaagg agcatggggt atatgcattt aattaaaaaa
     1801 tttataaatg gctaaaaaag tatttgcaat aaaactaggc agttatatat ttatcaagcc
     1861 atatccccgt tttgaaaacg ggatatcagg ttttcactga ttggaatagc agtcattatg
     1921 tgttattatt atagtcatat tgactaatta aattcctttt gttgaagcaa taggtttgag
     1981 attgtttcag tacaatcaac acatctgtag gggaagattt ccagtaagca atgcttattg
     2041 taaggacttc gtggtaagaa acattaatac aatttttgga ttaagtgaga atataatggc
     2101 ctatcaatta ttgctcagta aagaaaccct gaataaaatt ttacaataca aacagaacct
     2161 cgaaaaaggg cttgccacgc ccggtaaatt ttttcttgaa gaattaagta aacaagaaaa
     2221 aagcatctct gaaatggata ttaccacatt tactcaactg ttaatccagt ctaaaaaacc
     2281 acaggttttt gctgaaagtc aggtttatca tgatggaact gactggacac tggaagaaga
     2341 gagcattctt ggggatgtta gtgtcaatat gccagtcact atgtacaatg atggtggaca
     2401 tggctcctct ttcaagaatc acccagagcc aatttccggt tatttggctt atgtgccagg
     2461 cgccctcttg gcttcgggca gtggaccgac ttcagatatg aaagaagtgc tagacaatgg
     2521 caaactaaat caagacaaat taaacgcttt gtacgagaga cgtttgttac ctcaattgat
     2581 ccattttaat gagttagcgc gacaaaatga aaagcaggca gcaattacta ttcccggtat
     2641 aggaacaggg tgtttttcag gcgcttatta tgatgtgatt aaaccctatg tcagaaacgc
     2701 attaatccat attcttgaga aacataaaga ttcgttacct tacattgata tcattcatta
     2761 tgacccttat atgggagatg aacctgctga aaagaagatt ggtcatatgt cttttcgtgt
     2821 gagtccttct ggtgtggttc gtggcacaac ggggcaactt gactacccct taggtagcaa
     2881 tccagatacg catatccttg tttcaattgt tgcctgggat catttttcct ggcccggaaa
     2941 tgattattgg ggcggcgctc gtcaaacaga tgatggtgtc aaggccgcta gcacggatac
     3001 catggggcaa gttacaggag caactggtgt ctatgataaa aaatggggtc gatatatgcc
     3061 tccagaatca tttactaaag accgtaaagg tatgagtgac tggggggatt atgtcagaga
     3121 aaatggcatt gtatttaatg gccctgtttt agctctggat aaaagcggta aattggatac
     3181 cttggaaaat gttgcaagtc gttccaaagc aaaagttgat actacgacca caattagtga
     3241 gctcgttcgt ggcatgttca gccttttttc ccatagtact acgacagagc cttctccaac
     3301 tattaaagag gaagaatcga aaaaatcggg tcctcagtaa taattttgac aaattcttat
     3361 agagtaacaa tgggtctgac ttttgaggtt ggaccatgtt actatcttta gggcttacta
     3421 actctctccc aatcttttcg tcaagaaatg ttttttaatt tgctcattta gtttatctaa
     3481 aatcctatat aaagatgtct tgccttgagc tttaatcaac aggctaccta ctggatatta
     3541 ttgttttttt gtaaggaggt ttgattatgt tttgatgcct ttttttaaag aacaatcata
     3601 tttaatcaac gcagggatta tttggcctgt attctcacag ccaagtttct ttttaatatt
     3661 ctcaaagtgt ctctcgaccg tgcgtttgga aatttcaaaa attgaggcaa tacgagacaa
     3721 attaaaatca ttaaagtata aatagatcat ttccttttct cgatgagtca gatctttggc
     3781 ggctaaaaaa ctaaaaggaa gttgattata atcaaataca ttatctttaa tgagttcggc
     3841 cattttttgc tgcgaagatc tcagaataat ttctttaaga ttatctttct tacttgtatt
     3901 tcctgagcta atcagttcat tcatctggat tatacattct ctgggtaagg ttaaaaagtt
     3961 atcttttgta tgcaatcttc tggctcttga agacaggttt tcag
//
LOCUS       DBNCWK010000182         3921 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.183, whole genome shotgun sequence.
ACCESSION   DBNCWK010000182 DBNCWK010000000
VERSION     DBNCWK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.183"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            534..980
                     /locus_tag="AC06IO_002713"
     CDS             534..980
                     /locus_tag="AC06IO_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HIO9875876.1"
                     /translation="MKSEQSTWLAVFDSTTCSIYDYSKSDLSLVKKIEHSENKLKDID
                     ITSDKPGRYQASGAAHGAFSQESDPKEIQIERFSKEIAKTLEHGSAVNAYEKLILASA
                     PHMHGLLLKNINRQVEKSIIHVIPKDLIHFSENDLLDYVNNELKPK"
     gene            complement(1335..2243)
                     /locus_tag="AC06IO_002714"
     CDS             complement(1335..2243)
                     /locus_tag="AC06IO_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIO9875877.1"
                     /translation="MNILSCLKGFIATVDCKSFSKAAQKLYISPSKLSKQITWLEEEL
                     KVTLLIRSTTRLTVTDSGHRLYEKSLQLFEQLEAVKVIATPDQLELNGVIRLYLTVTP
                     AIPYLTKLSIQFMRQYPKIEVDIIVGSDTKELYSSTFDLAISFDDVKHSKLVCKRLFS
                     IQRKVFASPGYISQHGLPQTLDELPKHNCLINTLYGLQNKWVFNKKIIHVSGNFKSNN
                     AGVLKQAAVADVGILWAPYFSVYEEIKNGALTPVLPHESSPDINLYAICPKSTVDEQK
                     INLLLNFFCEKALSENIATSFIDGNN"
     gene            complement(2489..>3921)
                     /locus_tag="AC06IO_002715"
     CDS             complement(2489..>3921)
                     /locus_tag="AC06IO_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg2382 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875878.1"
                     /translation="NFLNRIERCLVPQSNPQIMKQLLSALGLDKLTTGNEEYKLFIQT
                     IKKKSIDLNPLISKVKSDILNNDPLWTLLAYIEENELIDDAEIENASSTIQLQINLLC
                     LLEAITITMANSNDFAHDIYNHMMKQRGKGATGNPFYNFLFGIPSRATLFERLKLISI
                     DPGLTSIIFHRLMGANPETQADLNDFVSQNRLSGWNADIELANFDVASTSTLPAMGVN
                     ILEAVWEDATGHNKNTGGILNAQAGLGLIGVMEEQEYTTSFKLAETTLPQGTTLHSDL
                     GYSLMPELKVDNSPKKVSQFHIDKQWMNLYNSWNLLFVIGNIDSVFVPLKLLMPSVFS
                     AEPSNYKETRVLTLFLVANLFLNQQTLNNPLFSNNFSFRNGAAIMKKWGEINKNHASE
                     LLQQCCPEKCKELDDLYNHVLGKNPHLNFGLKLFSYFQDSHQFRLTKKAQPSTSSDYS
                     FFSGNTPSENNSEKMGVALHSSLYTK"
BASE COUNT         1288 a          633 c          711 g         1289 t
ORIGIN      
        1 cgtttgcaga gaagagttat gggaaacttt gtctctgaaa aatcgaagag attggtagag
       61 tcgaaattgc tctaaatcgg gttagctatt ggtttgaaag cccaattcat aatggcatac
      121 cagttatctt attgttgaga tgaattgaaa tatctgggct gctccataga ctggcatggg
      181 ttaagcttcg ttgatttgcg gtgatctgca tttaaaaatg caaaaataat tgttgaacaa
      241 agaaaatgct atacttaaaa aacaaataat ttgtacgcct tgaaaaaaat atggatatgg
      301 cttgctgtat ttgaatgaga tccaactatt ttggacttat tcaaataagt tgataatttg
      361 gctccaaggt aatatttatc aggcagaagc atactacgag gcaattttat aaagcccaag
      421 tcaaatattg acacaaaaat ataggcttgg tcatcttgat aggtgatagc tgtttgactt
      481 ttgatgttat taaagttcat agatggaatt ctcattccgg gcatggaggt aatatgaaat
      541 ccgaacaatc aacatggctt gcagtttttg actccactac ttgcagcata tatgactatt
      601 caaaaagtga cttatcttta gttaaaaaaa tagaacattc agaaaataaa ttaaaggata
      661 ttgatattac ctctgataaa ccaggtcgat atcaagcttc aggggctgct catggtgcat
      721 tcagccaaga atcagacccc aaagaaatac aaatcgagag gttttcaaag gaaattgcca
      781 agacacttga acatggtagc gccgtgaatg catatgaaaa attgatatta gcttctgctc
      841 cgcatatgca tggtcttcta ttaaagaaca tcaacaggca agttgaaaaa tcgataattc
      901 atgtaatacc taaagattta atccatttct ctgaaaatga tcttttagat tatgtaaata
      961 atgaattgaa gccaaagtaa caggaagccg gttaacaagt gagttttgag taaatttgat
     1021 aggtgttaaa taaataggac acaggatttt ctgctcttaa atggtttcag tatggtaaca
     1081 atacgttcta tcagaatcag agtggataag acattatttt tggctttgcc taataaacgg
     1141 ttgaacagta tctaatttat ccttgcttcc tcaagtattg aaggacatgg cttgaatttt
     1201 gattgtcaaa tggtactaac tcattaaaga ttcatcacac aaccctaaat tccatgcgtc
     1261 tgttgaagtt ttagggtaat acaatcattc ttgtgtattt tctgagtatc aaagtcaaaa
     1321 gttctaatcc atctttaatt atttccatca ataaaggatg tcgcaatgtt ttctgacaaa
     1381 gctttttcac aaaaaaaatt caaaagtaaa ttaatttttt gttcatcgac tgtggactta
     1441 gggcaaattg cataaagatt aatatctggt gaagattcat ggggcaatac tggagttaat
     1501 gctccatttt ttatttcttc ataaaccgaa aaataaggcg cccatagaat ccccacgtct
     1561 gctactgcag cctgttttaa aacaccagca ttgttgcttt taaaatttcc cgagacatga
     1621 atgatttttt tgttaaaaac ccatttattt tgtaggccat agagagtatt aatcaaacaa
     1681 ttatgctttg gtaattcgtc gagtgtttga ggaagtccat gttgagaaat ataaccaggt
     1741 gaggcaaaga cctttctttg aattgaaaat aatcttttgc aaaccagttt tgaatgtttg
     1801 acatcatcaa atgaaatagc cagatcaaag gtagaggagt aaagctcttt tgtatccgaa
     1861 ccgacaataa tatctacttc aatcttgggg tattgtcgca taaattgaat acttaatttg
     1921 gttaaatagg gaattgccgg agtgacagta agatagagcc tgattacgcc atttaattcg
     1981 agctgatcag gtgttgcaat gactttgact gcctctaatt gctcgaaaag ctgcaatgac
     2041 ttttcataca acctatgacc tgaatcagtt acagttaatc gtgtcgtaga gcgtatcaat
     2101 aaagtaacct ttaattcctc ctccagccag gttatttgtt tgctcaattt agagggggat
     2161 atatataatt tttgtgctgc ctttgaaaag cttttacaat caacggttgc aataaagcct
     2221 ttcaaacagg ataaaatatt catcttaaaa ttttcttaat cgagatcttt ttaaatggta
     2281 tagacctata taccagtttt gtaaatcaat cacataccta ctgacataga ccaacgctat
     2341 tagtttattc catgaaaaca gcagttaaag cggatagtcg ggtatcctcg tgaaagattc
     2401 cagaaagaaa ttttttctca aaaacggaga ccatctataa tttgtctccg attctgatat
     2461 ttatttgctg ggtataaagc ataatatttt attttgtata taaagaacta tgaagggcta
     2521 cacccatttt ctctgaatta ttctcagatg gggtattacc tgagaaaaaa gaataatcac
     2581 tggatgtact tggttgtgct tttttagtga gtctaaattg atgtgaatcc tgaaaatatg
     2641 agaaaagttt taatccaaag tttaagtgag ggtttttacc gagtacgtga ttatataaat
     2701 catccagctc tttgcatttt tccggacaac attgttgcaa gagttcactg gcatgatttt
     2761 tattgatttc accccatttt ttcataatgg cagctccatt tcgaaaggaa aaattattcg
     2821 aaaacagagg gttattaaga gtttgttgat ttaagaagag attagcaaca agaaataagg
     2881 tgagcactct cgtttcttta taattggatg gttccgctga aaacacactg ggcattaaca
     2941 acttaagtgg tacaaataca gaatcaatat ttccaatcac aaacaataaa ttccaactat
     3001 tgtataaatt catccattgt ttatcgatat gaaactgaga tactttttta ggtgagttat
     3061 caacttttaa ctcaggcatc aatgaataac ctaaatctga atgaagtgta gtgccttggg
     3121 gcaaagtagt ttcagctaat ttaaaagaag tggtgtattc ttgttcttcc atgacgccaa
     3181 ttaatcccaa gccagcttgt gcatttaaaa taccaccggt atttttattg tggcctgttg
     3241 cgtcttccca caccgcttct aaaatattaa ctcccatagc gggtaaagta cttgttgatg
     3301 caacatcaaa attagcaagt tctatatcag cattccaccc ggataaacga ttttgactta
     3361 caaaatcatt gagatcggct tgagtttcgg gatttgctcc catcagtcgg tggaatatta
     3421 tgcttgtcaa accaggatct atggaaatca gttttaatct ttcaaataag gtggcacgag
     3481 aaggtattcc aaataaaaaa ttgtaaaaag gattacctgt tgcgcctttt cctctttgct
     3541 tcatcatatg gttataaata tcatgagcaa aatcgttact gttagccatt gtaatggtta
     3601 tcgcctctaa aaggcataat aaattaattt gaagctgtat cgtagaagaa gcattttcga
     3661 tttcagcgtc atcaattaat tcgttttctt cgatataggc aagtaaagtc cataaagggt
     3721 cattatttaa aatatctgat ttgaccttgc taattaacgg attcagatca atgcttttct
     3781 ttttaattgt ttggataaaa agcttatatt cttcattacc agtggttagc ttatcaagcc
     3841 caagagctga taataattgt ttcattattt gaggatttga ttggggtaca agacatcgtt
     3901 caatacggtt aagaaaattt a
//
LOCUS       DBNCWK010000183         3909 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.184, whole genome shotgun sequence.
ACCESSION   DBNCWK010000183 DBNCWK010000000
VERSION     DBNCWK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.184"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            11..295
                     /locus_tag="AC06IO_002716"
     CDS             11..295
                     /locus_tag="AC06IO_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HIO9875879.1"
                     /translation="MSNRKNKTIVIEGVTSQGKTFRPSDWAERMSGSLAVFKNSRIYY
                     SPLLQPSVNSEGYKCVLLDPKLKESSPQVYQAIMDFAKANNLKICGEEDL"
     gene            complement(439..981)
                     /gene="dotV"
                     /locus_tag="AC06IO_002717"
     CDS             complement(439..981)
                     /gene="dotV"
                     /locus_tag="AC06IO_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein DotV"
                     /protein_id="HIO9875880.1"
                     /translation="MNTIDLITILGNISQSLYPVQKLITGGAYILGVLFFLTAIGKLK
                     KIGDHRAQSSSQEKMFTPMMYLLVGALLIYLPSGMDLMANTAFGVGNVLTYSNYNPAN
                     IYSSMGFLIRTAGVLWFIRGCVLVAHSSQPGTQQGLKGLVFLIAGVFAMNFDNTIAMI
                     NSALDYFVKATLSVKAAAGF"
     gene            1065..1799
                     /locus_tag="AC06IO_002718"
     CDS             1065..1799
                     /locus_tag="AC06IO_002718"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HIO9875881.1"
                     /translation="MQINTFPITLEESFMISPKVKHFIFNCELSPPFEYLPGQFITIH
                     FEHDGKNLKRSYSIANEPKKDNRIEFAAGYFEGGPGTELLYNLKPGDVIHINGPFGRL
                     IFKDETPGRYILVATSTGTTPYRAMLNELGQRIEKHPDLHVVILQGVQRSEEILYPDD
                     FQAFAKKYPQASFRPYLSRVQKQDLKDNEYSGYVQHAFPELNLNPTRDMVYLCGNPGM
                     IDEAFNYLKEHGFPMQHIVREKYISR"
     gene            1949..2959
                     /locus_tag="AC06IO_002719"
     CDS             1949..2959
                     /locus_tag="AC06IO_002719"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIO9875882.1"
                     /translation="MSYEELSNTMEQMLHEGKGILAADESNSTIGKRFASINTENTEE
                     NRRDYRLLLATTPDLEQYINGVILFEETFTQTDEHGTPIPELFANKGIIPGIKVDKGL
                     VDLANTEHEKVTQGLDGLTERLLHFKKLGAKFAKWRNVYSISEYTPSLTAIKTGAENL
                     GRYAAACQSVGIVPIVEPEVLMDGNHSIEHCAEVCEMVLHELFHTLFIHQVELEHIIL
                     KPSMVTCGKEVTPFSDPEEVADYTISVFRNNVPASVPTINFLSGGQTPQQATANLNAI
                     NQIGHQPWLLSFSFGRALQEDCLKEWCGNKANIAKAQEALIKRARLNSMACLGEYQND
                     ME"
     gene            complement(3135..3890)
                     /locus_tag="AC06IO_002720"
     CDS             complement(3135..3890)
                     /locus_tag="AC06IO_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIO9875883.1"
                     /translation="MQPEVRGVSLITYNIHKGFGVGAVRFLLPEMRDAIAGLNPDFVF
                     LQEVQGKHRKREKRIVSWPDSPQCEYIAENIWPHYVYAKNAVYQSGHHGNAILSKYPF
                     ESFESINLSNMNRASRGILHTQLKLDNNIIHLLCVHLGLFKAERTEQCKALIQRIKDV
                     VPKNEPLIMAGDFNDWRHVISKILAENLGIEEAFVALEGQHARSFPAIKPALCVDRVY
                     FRGMKVQEVACLQGKPWRMLSDHLPLYARFELL"
BASE COUNT         1285 a          763 c          716 g         1145 t
ORIGIN      
        1 gcatagagtt atgtctaatc gaaaaaataa aactattgtc attgagggtg ttacttcaca
       61 aggcaaaaca tttcgcccaa gtgactgggc cgagcgtatg agcggttctt tagctgtttt
      121 taaaaatagt aggatttatt attctccgct cttgcaacca agcgttaata gtgagggtta
      181 taaatgcgtt ttacttgatc ccaaactcaa ggaatcaagc ccacaagttt atcaggcaat
      241 tatggatttt gctaaagcta acaacttgaa aatctgtggt gaagaagact tataacattc
      301 caaaggattg ccctccagtt ttattttctc aggatttagg aaaaccccca atttcttggt
      361 taaaaacatt tttgcctcga tcttagggtt tttcctaagt cttgtttctt taaagcgcag
      421 acaaatcaat cagaaaaact agaatcccgc tgcggctttt actgataaag ttgctttgac
      481 aaaataatct aaagcagaat taatcatggc aatcgtatta tcaaaattca ttgcaaaaac
      541 gcctgcaata agaaatacca atccttttaa tccttgttga gtacctggct ggctggaatg
      601 agcgacaagt acacatccac gtataaacca cagaacaccc gccgtgcgaa ttaaaaaccc
      661 cattgaactg tagatattgg caggattata attggaataa gttaatacat taccgacacc
      721 aaaagcggtg tttgccataa gatccatacc cgaaggcaag tagattaaaa gtgccccaac
      781 taataaatac atcattggag taaacatttt ttcctgtgag gaggattgag cccgatgatc
      841 cccgattttt tttaacttgc caattgccgt taaaaaaaat aagacaccca atatataagc
      901 tcctcctgtg ataagttttt gcacaggata aagggattgg ctaatattac ctaatatagt
      961 gattaaatct atcgtgttca tatgttaatt atatgcgctt agctcttgca atgcataatt
     1021 tttcccctat attaatcatt ttagaataaa agttgatatt ccttatgcaa ataaatactt
     1081 tccccatcac tttagaagag tctttcatga tttctcccaa ggtaaaacac tttatattca
     1141 attgtgaatt atcaccccct tttgaatatt taccaggaca atttattact attcattttg
     1201 aacatgatgg aaaaaactta aagcgtagtt acagtattgc caatgaaccc aaaaaggata
     1261 atcgcattga atttgctgca gggtatttcg aaggaggacc aggcacagaa ctactctaca
     1321 atttaaagcc aggtgatgtg atccacatta atggaccttt cggtcgtttg atttttaaag
     1381 atgaaactcc aggaagatac atattggtag cgactagtac tggtaccaca ccttatagag
     1441 ctatgctcaa tgagcttggt caaagaatag aaaagcatcc tgatttgcat gtagtcatcc
     1501 ttcaaggagt gcaacgcagt gaagagattc tgtatccaga tgattttcaa gcctttgcaa
     1561 aaaaatatcc tcaagcaagt tttcgtccct atttaagccg tgtgcaaaaa caagacttga
     1621 aagacaatga atacagtggt tatgtacaac atgcatttcc agagcttaat ttaaatccaa
     1681 ctcgcgatat ggtttattta tgtggaaatc caggtatgat cgacgaggca tttaattatt
     1741 taaaagaaca tggttttccc atgcaacata ttgtacgtga aaaatatatt tcacgctgaa
     1801 ataccgcatc aaaaaccatt gttaatcagt gtcatccttt cggtaaataa cccggatttg
     1861 acaaaagatg actagtcgtt atttaaactt aataggtgcc aaaccacttc gaaaggtcga
     1921 cacccgataa tgaggagata aacacattat gagttatgaa gaattatcaa ataccatgga
     1981 gcaaatgctg catgaaggaa aaggtatttt agctgcagat gaaagcaaca gtacaattgg
     2041 caagcgcttt gcgtcaatca atacagaaaa taccgaggaa aatcgaagag attatcgctt
     2101 gttactagcg accactcctg atttagaaca gtatatcaac ggcgtcattt tatttgagga
     2161 aacttttact caaactgatg agcatggaac accaattcct gagctttttg ctaataaagg
     2221 cattattcct ggaattaaag tagacaaagg gcttgttgat ttagccaata cagaacatga
     2281 aaaagttacc cagggcttag atggtttaac tgagcgtctg cttcatttta aaaaattagg
     2341 tgcaaaattc gcaaaatgga gaaacgttta ttcaatttcc gaatacactc ccagcctaac
     2401 tgcaatcaaa acaggagctg aaaatttagg ccgttacgct gctgcttgtc aatcagtcgg
     2461 catcgttcca attgttgagc ctgaagtatt aatggatggc aaccacagta tagaacattg
     2521 tgctgaagtc tgtgaaatgg ttctccatga gctgtttcat accttgttta tccatcaagt
     2581 ggagttggag catattatac ttaaaccgag tatggttacc tgtggtaaag aagtgacccc
     2641 gtttagcgat cctgaagaag tggctgacta caccattagt gtatttcgta ataatgttcc
     2701 agcgtcagta cccaccatta atttcctctc cggtggccaa acaccccaac aggctaccgc
     2761 taatttgaat gcaatcaatc aaattggcca ccaaccctgg ttattaagtt tttcatttgg
     2821 aagagcatta caagaggatt gcttaaaaga atggtgtgga aacaaagcaa atattgccaa
     2881 agcgcaagag gctttaatta agagagcccg cttaaacagt atggcttgtt tgggtgaata
     2941 ccaaaacgac atggagtaaa gttgcaatag cgacagtacc ttctctcgac caggaagact
     3001 gtcgctttaa ttttaatcat ataatgctta ctaaatcccc atggtcaagg caaaaaattt
     3061 taaatgaggg agttggaata aaccaaatga ccgcattaaa aatcttttca acaaagagac
     3121 tggaggtagc actgtcataa taattcaaaa cgtgcataca agggtaaatg atcagataac
     3181 atccgccaag gtttgccctg taaacaagca acctcttgaa ctttcatccc gcgaaaatac
     3241 accctgtcga cacacaaagc aggttttatt gcaggaaatg aacgggcatg ttgtccttcg
     3301 agcgcgacaa aagcttcttc aatccctaag ttttcagcaa ggattttaga aataacatga
     3361 cgccaatcat taaagtctcc tgccattatc aaaggctcat ttttaggcac aacatcttta
     3421 atacgttgta tcaatgcctt acattgctcg gtacgctctg ccttgaatag gcctaaatga
     3481 acacacaaaa gatgaataat attattatcc aattttaatt gggtatgaag tatacctctt
     3541 gatgctctgt tcatgtttga aagattaata ctttcaaaac tctcgaacgg gtatttgctt
     3601 aaaatagcat ttccgtggtg gccagattga tatactgcat tctttgcata aacgtaatgt
     3661 ggccatatat tctcagcaat gtattcacat tgaggggaat caggccaact gacaattctt
     3721 ttttcgcgtt ttctatgttt accttgcact tcttgtaaaa aaacaaagtc cggatttaat
     3781 ccagcgatag catctcgcat ttctggaagc aaaaacctta ctgcacctac tccaaaacct
     3841 ttatgaatat tatatgttat cagcgatacg ccgcgcacct caggttgcat agatactcta
     3901 tctctcatt
//
LOCUS       DBNCWK010000184         3709 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.185, whole genome shotgun sequence.
ACCESSION   DBNCWK010000184 DBNCWK010000000
VERSION     DBNCWK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.185"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            93..1238
                     /locus_tag="AC06IO_002721"
     CDS             93..1238
                     /locus_tag="AC06IO_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HIO9875884.1"
                     /translation="MLRWIVLICISLFLPLSAYADKSDCINHHCIAVIDAGSSSSRLH
                     IYAYDLDETNTPIAIREIWNRKAVPGLSTIEHENKSVSAYMEKLFSDAPAVLMPVYFY
                     STAGMRLLPKTHHRNVNQLVAEWFDRQYYWRLVTARTISGTEEGIFAWLATNYQLNLF
                     QQEPQTLAGVMDIGGASVQIVIPVVNPSFMDHEHTTTIRLYGKEFTVFSHSFLGLGQN
                     EMGHQFFDLKFCFPEEYELPSGRQARGDAYACKDEIAILVNSVHSVSRKIKSVLAANP
                     IEKWYILGGITFMLNDKLFSLDNQYFTNRDLISFADQSICHQKWSTLEELYPDNLMVF
                     NYCMLPSYLYALLVHGYGLKSYQKVNFMPNSKNIDWTLGVVLQHKQS"
     gene            1255..1327
                     /locus_tag="AC06IO_002722"
     tRNA            1255..1327
                     /locus_tag="AC06IO_002722"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1288..1290,aa:Thr,seq:cgt)
     gene            complement(1450..3342)
                     /gene="rmuC"
                     /locus_tag="AC06IO_002723"
     CDS             complement(1450..3342)
                     /gene="rmuC"
                     /locus_tag="AC06IO_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIO9875885.1"
                     /translation="MQDIQWTTELISIAIIIGITLTTIGFLLAMVLQGRKLTYITTQL
                     EHSQKTIEQTNNTNTEIQAQLKNTQSTLHQKELLESKLQEQFSNAKANEQRLASELAM
                     LKDELTDLGSKLEHMHQQMHISEKKYEAAQAENRALQKQASDLDQRLTQTNQALEKER
                     NLVSELKDYLSQEGKKTKSLEVSEKEAREQLNEIKLKMSEQITDYRQLQERNQSLSNE
                     YTELKTTLERKEEHFKEQMQQLNEAKLALTREFENLANKIFEEKGKAFTHNSQVSIDN
                     ILKPFREQIEGFQKRINEVHDASIQGNTSLSFEIKKVLDIGLQMSKEANNLTSALKGN
                     SQQRGAWGEAQLRRTLEMSGLIENTHYEVQSSFKDSEGKQKQTDYLIKLPDGKHIIID
                     SKVTLNAYDRAVAAESPEEYQLAMNEHVKAVRKHIDDLTSKDYTNLIGMRSPSFVLMF
                     MPMEPAYIEALKSNKDLFEYGYNKGIVLVSHTTLIPILRTVSNLWMIERSNAEAREIS
                     EKAGEIYNQVCIVAERLSKLGSTLSIASNHYNNTVKALVGQQGLYGKVDRFSQLSAKV
                     SKSMPQLEPSHMDFETERLSLIIEPIDEEQVQSASLKIENKSDTTSVTAPTDDPNIGR
                     KSKTEN"
BASE COUNT          955 a          716 c          730 g         1308 t
ORIGIN      
        1 cgcttttatt ctcaaattca gttcctgttt tgaccaaaaa aaacaattaa tgtaggataa
       61 atgcgttatt tgaggtgaat taaggaaaga ctatgctgcg atggatcgtg ttaatttgta
      121 tctctttgtt tttgccttta tctgcttatg cggacaaatc cgattgtatc aatcatcatt
      181 gcattgcggt aattgatgct ggaagttcca gttcacgatt acatatttat gcctatgatt
      241 tggatgaaac aaacacacct attgcaatta gagagatttg gaaccgaaaa gccgttcccg
      301 gattgtctac aattgagcat gaaaacaaat cggtttcagc ctacatggag aagctatttt
      361 cagatgctcc agctgtttta atgccagttt atttttactc cacagcaggc atgagattgt
      421 tgcctaaaac tcaccacaga aacgtcaatc aattggtagc tgagtggttt gacaggcagt
      481 attactggcg acttgtgact gcaagaacga tttctggtac agaggagggt atttttgcct
      541 ggctcgcaac caattatcaa ctaaacctgt ttcaacaaga accccaaact ttagctggtg
      601 tgatggatat tggcggagca tctgttcaga ttgttattcc tgttgtaaat ccatctttta
      661 tggatcatga gcatacaacc acaattcgcc tctacggcaa ggaatttacg gttttctctc
      721 atagttttct tggtcttggt cagaatgaaa tggggcatca gttttttgat ttaaaattct
      781 gctttccaga ggaatacgaa ctgccaagcg ggcgccaagc tcgaggcgat gcctatgctt
      841 gcaaagatga aattgccatt ttagtcaata gcgttcattc tgtaagccga aagattaaat
      901 cggttttagc cgctaatcct attgagaaat ggtatatttt gggcggcatt acctttatgc
      961 tgaatgacaa actgttttct ctggacaatc aatattttac caatcgtgat cttatttcct
     1021 ttgcagatca aagtatttgc catcaaaaat ggtcaactct tgaagagctc tatcctgata
     1081 acctcatggt gttcaactac tgcatgctgc cttcctacct ttatgctttg ttagttcacg
     1141 gctatggact taagagttac cagaaagtta atttcatgcc aaatagcaag aatatcgatt
     1201 ggaccttagg agtggtattg caacataaac aatcttaagt atacttttct ttatgccgtt
     1261 gtagctcagt tggtagagca attgattcgt aatcaagagg tcggaggttc gactcctctc
     1321 agcggcatta gtactgacaa ggcctatggc gaattttttg tgtcgcatct aaagtgcttt
     1381 catcaaatga gaaccatttc aactattaac ttaatgagga ctgcttactc ctttactggg
     1441 atagagcccc taattttcgg tctttgattt tcttccaatg tttggatcat ctgtgggagc
     1501 tgttactgag gtagtgtccg acttattttc aatttttaga gatgcagatt gtacttgttc
     1561 ttcatctatg ggttcaataa tgagcgataa gcgttcggtt tcgaagtcca tatgagaagg
     1621 ttccaattgt ggcatgctct tgctaacttt cgcagacagt tgactgaagc gatccacctt
     1681 tccatacagt ccttgttgcc ctaccagggc cttaacggtg ttattgtaat ggttactggc
     1741 aatactgagt gtgctaccaa gtttacttaa acgttcggca acgatgcaca cttgattata
     1801 aatttctcca gctttttcgc ttatttcgcg agcctcagca ttgctgcgtt cgatcatcca
     1861 caagttagat acagttcgta aaatagggat caaggtagtg tgggagacta atactatgcc
     1921 tttattgtag ccatactcga acaggtcttt gttgctttta agcgcctcaa tatatgctgg
     1981 ttccatgggc atgaacatta gtacaaaact agggctgcgc atgccgatca gattggtata
     2041 gtctttggag gtcaaatcat caatgtgttt gcgcactgcc ttaacatgct cattcatagc
     2101 taattgatat tcctctggtg attcagcagc cactgctcta tcgtaggcat tcaaagtcac
     2161 cttactgtca ataatgatgt gttttccatc gggtaattta attaggtagt cagtctgctt
     2221 ttgtttacct tctgaatctt taaatgagct ttgtacttca taatgtgtat tttctattag
     2281 accactcatt tcgagggtgc gtcggagttg ggcttcaccc caagcgcctc gttgttgtga
     2341 gtttcctttt aatgcggaag ttaaattatt tgcctctttg ctcatttgca agccaatatc
     2401 taataccttt ttgatttcga agcttagact agtgttacct tgaattgaag catcatgcac
     2461 ttcattaata cgtttctgga aaccttcaat ctgctctcga aatggcttaa gtatattatc
     2521 gatgctaacc tgactgttgt gggtgaaagc ctttcccttt tcctcaaaga ttttattggc
     2581 taggttttcg aactctcttg taagcgctag tttggcctca ttaagttgct gcatttgttc
     2641 tttaaaatgc tcttctttac gttctaatgt ggtctttaat tcagtgtatt cgttgctcag
     2701 gctttggttg cgctcttgta attgcctata gtcagttatt tgctcggaca ttttcaactt
     2761 tatctcgtta agctgttcac gagcttcttt ctcagatact tcaagagatt tggttttttt
     2821 accttcttga gacaaatagt ctttgagctc acttactagg ttgcgttctt tttccaaggc
     2881 ttgatttgtt tgggttaatc gttggtctag atcacttgct tgtttttgta gggctcggtt
     2941 ctctgcttga gctgcttcat acttcttttc agatatgtgc atttgttggt gcatatgttc
     3001 taattttgac ccaagatcgg ttagctcgtc tttgagcatc gctagttcgc ttgctaatcg
     3061 ctgttcatta gcttttgcat tactgaactg ttcttgtaac ttgctttcta ataactcttt
     3121 ctgatgtagg gtggactgtg tatttttcag ctgtgcctgt atttcagtat tagtattgtt
     3181 tgtctgttca attgttttct ggctatgctc caattgcgta gtgatatacg ttagttttcg
     3241 accttggagt accatcgcta acaaaaagcc gatagtcgtt aaggtgatac caatgatgat
     3301 agcaatgctg atcaactcag ttgtccattg aatgtcttgc atatttactc ctgtatttag
     3361 tatgctgaaa tccaatcttt taaaagtaat ttaagcaaac gggctactct atatttcttc
     3421 aaagctagtg gacaataaac aagaaaaaac aataaagctc ggatcgttct caatacattt
     3481 caataaaact ccaactaatt cattagagct atcggatttt aatattttat acgttactct
     3541 tattccacca gcatcatgac aagcatctga catactatta aatacattgt ttgcagtctt
     3601 aggttcaact gtaattctat atgcttgttc acattaaggc ggttgtccac taagaggaag
     3661 taaaagtttt tctgcaaatg cattataatt aattaaaata aagacaagg
//
LOCUS       DBNCWK010000185         3669 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.186, whole genome shotgun sequence.
ACCESSION   DBNCWK010000185 DBNCWK010000000
VERSION     DBNCWK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.186"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1099
                     /locus_tag="AC06IO_002724"
     CDS             <1..1099
                     /locus_tag="AC06IO_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875886.1"
                     /translation="ILLSIEQYKSHANKIEAIINILRPSILLFSEDIVERDSNLWIKI
                     AHTKNILSMVISYGTPSKTEAAETYCNDPNYQFPTHLPPHIKFLLKFVLNKWCFLHQK
                     RKLIRLPLKQLVAMDLLDYSPKLPWVTNSGEADIVMVESNFAKDLFKKEGLKNKRIKV
                     LGNLKFDAMNTILNDLKSYKAYIYEKYNLCRDRNLILCAFPSYLPERKLLDFNSYEDL
                     IYKYVLGLQASNHHVLYSIHPSGIPWVGDKLRHLGQLVAEEKIFNLIPICDIFLATVS
                     STIKWARACGKLVLNYDCFGFNYDVYKKDSAVIHINSFDELLVWVDKLNNQQVSDILM
                     ELSPFIPSNYWGKIDGQSFHKIRYYIDALAK"
     gene            complement(1445..2520)
                     /locus_tag="AC06IO_002725"
     CDS             complement(join(1445..2271,2271..2520))
                     /locus_tag="AC06IO_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611486.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIO9875887.1"
                     /translation="MKKRYSEEQIIKAIKSHESGVKVEEICRDYSISSGTFYNWRSKY
                     AGFEVNEAKRLRDLESENTKLKKLLADKLLELEAMKDVLFKKVVKPAAKKPIVSHLIS
                     KFKLSECAACRLAGLSRISFRYQSKSRTDEALRERLKELACQYPAYGYLMLHGLLKAE
                     GLVTNKKHTYRLYSEEGLQVRTKKRKKLIRPRQPIEIPATVNQRWSMDFVSDQLSNGR
                     RFGILNVVDDYSREVIGQLVSVSISGRLVARFLNNLIEERNKPTKIICDNGSEFTSKA
                     MFFWSKETNVGLGFIQPGKPTQNAFVESLNGKFRNECLNQHWFRTLEEAKYEIEKWRQ
                     HYNYVRPHSSLNYVAPVEFARQVA"
     gene            complement(2705..>3669)
                     /locus_tag="AC06IO_002726"
     CDS             complement(2705..>3669)
                     /locus_tag="AC06IO_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HIO9875888.1"
                     /translation="QDSYIGKRVIDMIRATKQVETPPFPRQIQVETTNICNHNCEFCA
                     YTVMERPKRQMDPDLFKRLVKEAYECGAREIGLFSGAEPLTCKYLTEYIDYCRRLGYE
                     YMYLSTNGSVPDKNKFKQILDAGLNSIKFSVNGGNRAIYKQIHGNDHFERVIDNIKFV
                     SQYRCNVKQQVYLAVSFVGMDSSKETFEELKQILDGYVDEILYYDAANQSGQMPHLPL
                     PPYRECHLPFSKLHISREGYLRACCNDYENLLAMEDLNITPMKEAWNSKLFQELRSKH
                     INNKLDGILCGNCIRASKIKACAINPELIHQKDNKLKQIPIIAE"
BASE COUNT         1133 a          680 c          603 g         1253 t
ORIGIN      
        1 gatattgtta agtattgaac aatataaatc ccatgctaat aaaatagaag caattattaa
       61 tattctcagg ccatctattt tattatttag tgaggacata gttgagcgtg atagtaatct
      121 atggataaag atagctcaca caaaaaatat tttatctatg gtgatttctt atggaacccc
      181 ttcaaaaact gaagctgcgg agacatactg taatgatcca aattatcaat ttcccactca
      241 tctaccgcct catattaaat ttcttttgaa atttgtttta aataaatggt gttttctgca
      301 ccaaaaaaga aaacttattc gccttcctct gaaacagtta gttgcaatgg atttattaga
      361 ttattcgcca aaattacctt gggtaacaaa tagtggtgag gccgatatag ttatggttga
      421 aagtaatttc gctaaagatc tattcaaaaa agaaggactt aaaaataagc gaattaaagt
      481 tttggggaat ctaaaatttg atgccatgaa tacaatattg aatgatttga agagctataa
      541 ggcctacata tatgaaaaat ataatttatg cagagaccga aatttaattt tgtgtgcctt
      601 tccttcttac ttacctgaga gaaagctact agattttaat agctatgagg atttgattta
      661 taaatacgta ttaggattac aagcatcaaa tcatcatgta ctatattcaa ttcacccatc
      721 aggaattcct tgggttggag ataaattaag gcatcttggc caattagttg cagaagaaaa
      781 gatttttaat ttaattccga tatgtgatat tttcttggca acagtttcaa gcaccatcaa
      841 atgggctaga gcgtgtggta agcttgtatt aaactatgac tgttttggtt ttaattatga
      901 tgtatataag aaagattctg cagttattca tattaatagt tttgatgagc tcttagtttg
      961 ggtggataaa ctgaataacc aacaagtatc agatatcctc atggagctaa gcccttttat
     1021 tccatctaat tattggggga aaattgatgg gcaatcattt cataagattc gttattacat
     1081 tgatgctcta gctaaataac atcacctaga tataggcaga gctaaattct caccaagtga
     1141 atcgatttga gatagaagca gctaattctg atgagaagtt aataataaaa ttatcaggac
     1201 tttgatatct tgtcaactca atagctgttt ggacaatccc attgtatccc aaaaaattga
     1261 tttttggagc tcaatcagaa caaggtagtc gttttattga acgtatcttt tctgttgttg
     1321 ctacttataa acagcaaaaa aagatgtctt ggcatttctc atggaagcac ttcatcaatc
     1381 ggcttcaatt caaaagtgac ctttcccctg aattaatacc acaacattga tgagataaat
     1441 tatattaagc tacctgcctt gcaaactcca ccggcgcaac atagttaaga gaactatgag
     1501 gacgtacata gttataatgt tggcgccatt tttcaatctc atatttagct tcctcaagcg
     1561 ttctaaacca gtgttgatta agacattcat ttctaaattt gccatttaaa ctttcgacaa
     1621 aagcattttg tgtcggtttt ccaggctgaa taaagcctaa gccgacgttt gtttctttgc
     1681 tccaaaagaa cattgccttg ctagtaaatt cggagccgtt gtcacaaata atctttgttg
     1741 gtttatttcg ttcttctatc aggttattta gaaagcgtgc caccagccta ccactaatgg
     1801 atactgaaac tagctgacca ataacttcac gagaataatc atccactaca tttagtattc
     1861 caaaccgtct tccattactc agttgatctg aaacaaagtc cattgaccac cgttgattaa
     1921 ccgttgcagg aatttcgatt ggctggcgag gacgaatgag ttttttcctt ttctttgttc
     1981 tcacttgtaa gccttcttca gaatacaacc gataagtatg tttcttattt gtgactaaac
     2041 cttccgcttt aagaagtcca tgaagcatta aatacccata agcaggatat tggcaggcca
     2101 attcctttaa tcgctctctg agcgcttcat ctgtccttga tttggattga taccggaatg
     2161 atattcgact caatcccgcc agcctgcacg ccgcacactc acttaattta aactttgaaa
     2221 tgaggtggct gacaattggc ttcttggccg ctggctttac cacttttttg ataacacatc
     2281 cttcatggct tcaagttcaa gaagtttatc ggccagtagc ttcttaagct tggtgttctc
     2341 actttcaaga tcccgcaaac gcttagcttc atttacttca aacccagcat acttactgcg
     2401 ccaattataa aaagtacctg aagagatgct gtagtctcgg caaatttctt caactttgac
     2461 accagattcg tgggatttta tcgctttaat tatttgctct tcgctgtatc gctttttcat
     2521 cttgagatct cctattttta aattttatcg gaaatctcat ctttgtcatg gtcttatttt
     2581 tgggggatag gtcaaaagaa acttagacgc ccctttacaa ggggcgtgag cgtttacacc
     2641 taacagggcg gccgagttag atatttaaaa atattggata ataaaaacta tataaacaga
     2701 aaacttattc agcaattata ggaatctgtt ttaatttatt atccttttga tgaatgagct
     2761 caggattaat tgcgcatgct tttattttag aagcccgaat acaatttccg cataatatac
     2821 catccaattt attattaata tgtttacttc ttaattcttg gaaaagctta ctattccatg
     2881 cttctttcat aggagtaata tttaaatctt ccattgccaa taaattttca taatcattac
     2941 aacaagctct taagtatccc tctctactga tatgtaattt actgaaaggt aaatgacatt
     3001 ctcgatacgg aggcaacggt aaatgtggca tttgccctga ttgattagcc gcatcataat
     3061 aaagtatttc atctacatag ccatccaaga tctgctttaa ttcttcgaat gtttcttttg
     3121 atgagtccat acccacaaaa gatacagcta gataaacttg ttgtttaacg ttacaacgat
     3181 attggctgac aaattttata ttatcaatta ctcgctcaaa gtgatcattc ccatgaattt
     3241 gtttatatat cgctctattt cccccattaa cagaaaattt tatggaattt agccccgcat
     3301 caagtatttg cttaaattta ttcttatcag gaactgaacc attagtggat aaatacatat
     3361 actcgtatcc taatcgtctg cagtaatcaa tatattcagt taaatatttg catgtgagtg
     3421 gttctgctcc agaaaataaa ccaatttctc tagctccaca ttcatatgct tcttttacca
     3481 accgcttaaa taaatcagga tccatttgac gcttaggtct ttccataact gtataagcac
     3541 aaaactcaca attgtgatta caaatgtttg ttgtttctac ctgtatttgt ctagggaatg
     3601 gtggggtttc cacttgcttt gtggcacgaa tcatgtctat gactcgttta cctatataac
     3661 tatcttgtt
//
LOCUS       DBNCWK010000186         3537 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.187, whole genome shotgun sequence.
ACCESSION   DBNCWK010000186 DBNCWK010000000
VERSION     DBNCWK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.187"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(28..1470)
                     /locus_tag="AC06IO_002727"
     CDS             complement(28..1470)
                     /locus_tag="AC06IO_002727"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIO9875889.1"
                     /translation="MNFVTFQFVVFLVITVIVFMMMSPYYRKYFILLCSYFFYGTWSV
                     PLISVILFSTSVDYYLGKKIHESHSQTHKKLYLTLSILVNCSILVYFKYMNFFISSID
                     AIGFSLGATQLLLNPMHIILPLGVSFYTFEAISYIVDIYRGEKPASSWLNYNFYIMYF
                     PHLISGPIVRYKELHAQYESKIELPETSRLKEGFQLLLYGFIFKLIIADSMASISDPL
                     FDKYQTIGHLESWLASIAFTIQIYFDFLGYTHIARGVSLFFNIKLPVNFNHPYNARNI
                     SEFWQRWHITLSLWIRDYLYIPLGGNRGRLYQTVNNLLITMFIAGLWHGASWNYAIWG
                     IYHGCLLAVYHVFRFYYIKRNLQTENQLMKIGSIIMTYSLVVIGWVIFRSTSFEQAFT
                     ILGKMYDIPGFVQEFAMNQRASAYTDSLFYLVGMFALCFIGPTFKSIETKILSAIQPF
                     WIKKWLYSLSIFTVLIFMFSPVKPFIYFVF"
     gene            complement(1467..2633)
                     /locus_tag="AC06IO_002728"
     CDS             complement(1467..2633)
                     /locus_tag="AC06IO_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HIO9875890.1"
                     /translation="MSDTHCEKSYRSKLLKQGLLGVLILFLVFLFLESFSRWVAATST
                     APFGESIRNDQKMIVSDSIRQGIVAIGDSIIARSFYPELFSSLLAKNFSKPYEVYNIG
                     VEGSGIIHHRAHLRYLLKRNVKPDFVIMNVSVHNLNLNYFAHDSNEHIIEQRYEDHNS
                     LDYYNRCFTVNKQTLSSTLYCKIAKYSYFFRLVHYYHQQVNNSLQSLLHTQRLRTENL
                     IIKYPAISRQGFSPIFGSLAEDTISTPRMFEFLRHEYRSAFANFKLTTEVLDSIIDDL
                     NNNSIKVILVLAPVYHPVTRKVYDEFNLPSNGAIVSTLENYALKKNIAFINLFEECQD
                     PVYFQDPVHPNIYGAVKITTLLSQKIINKQFVGPDGYLDKELFQYLKSNNENKA"
     gene            complement(2877..3413)
                     /locus_tag="AC06IO_002729"
     CDS             complement(2877..3413)
                     /locus_tag="AC06IO_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIO9875891.1"
                     /translation="MISESVIPLAYFITFTCYGTWLHGGKSTSVDRYNNIPGTEFIPY
                     DRRRLYQVKNLMLETPYSLDELRRHIVLQAICEVCRYRQWVLLAAHVRSNHVHLVIHA
                     QISPEHIMNTVKAYASRYLNYTRLDGDRKNRWTRHGSTRYLWNEEEIEATIQYVVYEQ
                     GLPMAVFENKDRVFGVEV"
BASE COUNT         1286 a          628 c          576 g         1047 t
ORIGIN      
        1 tgagggagcg gataacaaat tatctaatta aaaaacaaaa taaataaacg gtttcacagg
       61 ggaaaacata aaaattaaca ccgtaaaaat tgaaagcgaa taaagccatt ttttaatcca
      121 gaatggctgt atagctgaca agattttcgt ttcaattgat ttaaaagttg gccctataaa
      181 acacagggcg aacattccca cgagataaaa caatgaatcg gtataagctg aagcacgttg
      241 attcattgca aattcctgca caaaaccagg aatatcatac atcttaccga gaatagtaaa
      301 tgcctgttca aatgaagtag atcgaaatat tacccagcct atgactacta atgaataagt
      361 cattataatt gagccaatct tcatcaattg attttcagtt tgtaaatttc ttttaatata
      421 ataaaaccgg aaaacatgat aaacggctaa caaacaacca tggtatatgc cccaaatcgc
      481 ataattccag gaagcgccat gccataaacc agcaataaac atagtaatta gtaaattatt
      541 caccgtttga tacaaacgac ctcgattacc tcctagagga atatataaat aatctctaat
      601 ccataatgac aaagtgatat gccaacgctg ccagaattca ctgatattgc gcgcattata
      661 aggatgatta aaattaacag gtaacttaat attaaaaaat aatgatacac cacgcgcaat
      721 atgggtatat ccaaggaaat caaaataaat ctgtatggta aatgcaatac tggccaacca
      781 ggattccaga tggccaattg tctgatactt atcaaacaag ggatctgaga ttgaggccat
      841 tgaatcggct atgatcaatt tgaaaataaa gccataaagt aataactgaa acccctcttt
      901 taaacgcgaa gtttccggta attcaatttt actttcatat tgagcatgca gttctttata
      961 acggacaatc ggtcctgaaa tcagatgcgg gaaatacata atataaaaat tatagtttaa
     1021 ccagctacta gccggctttt cccctctata gatgtctacg atataactga tcgcctcaaa
     1081 agtgtaaaaa gaaacaccga gaggcaaaat aatatgcata ggatttaaca aaagttgggt
     1141 tgctcccaaa ctaaagccaa tagcatcaat actggaaata aaaaaattca tgtatttgaa
     1201 ataaaccaga atggagcaat taaccaaaat ggataaagtc aaatagagtt ttttatgagt
     1261 ttgggaatga ctctcatgga ttttttttcc gagatagtaa tcaaccgagg ttgaaaataa
     1321 gatgacactg attaatggaa cgctccaggt accgtaaaaa aaatagctgc aaagcaaaat
     1381 aaaatatttc cgataatagg gagacatcat cataaaaaca atgaccgtaa taactagaaa
     1441 aacaacgaac tgaaaagtga caaaattcat gctttgttct cattattgct ttttaggtat
     1501 tgaaacaatt ctttatccaa atacccgtca ggccctacaa attgtttgtt gataattttt
     1561 tgcgataaca atgtggttat ctttacagca ccatagatat taggatgtac tggatcctgg
     1621 aaataaacag gatcctgaca ttcctcgaat aagtttataa aagcaatatt tttctttaaa
     1681 gcataatttt caagcgtact gactatagcg ccattagatg gcaagttgaa ttcgtcataa
     1741 accttacgag ttactggatg gtaaactggc gccaagacca agatcacttt aattgaattg
     1801 ttattcagat catcaataat gctgtctaaa acttcagtag ttaacttaaa atttgcaaat
     1861 gcactcctat attcatgtct caaaaattca aacattctag gcgtgcttat tgtatcctca
     1921 gcaagactgc caaaaattgg tgaaaaacct tgtctagaga ttgctggata tttgattatc
     1981 aaattctcag ttctcaatct ttgtgtatgc agcaaagatt gcaaactatt gttaacttgt
     2041 tgatggtaat aatgcactaa acgaaaaaaa taagaatact tcgctatctt gcaataaagc
     2101 gttgatgaaa gtgtttgctt atttactgta aaacaacggt tataataatc caaggaatta
     2161 tgatcttcat atctctgctc tatgatatgt tcattactat catgggcaaa ataattcaaa
     2221 ttaagattat ggacagaaac attcataatc acaaaatctg gtttgacatt ccttttcaat
     2281 aaatatctta agtgtgcccg atgatggata attcctgaac cttccactcc tatgttatac
     2341 acctcataag gctttgaaaa atttttagct aataaagaag aaaaaagttc cggataaaaa
     2401 cttctggcaa ttatggaatc cccaatagca acaatacctt gcctgatgga gtcactcaca
     2461 atcattttct gatcattcct gatactttca ccaaaaggtg ctgtacttgt agctgctacc
     2521 catcttgaga aagattcgag aaacaaaaaa accaggaata aaatcaatac accaagcaaa
     2581 ccctgtttga gaagttttga tctgtagctt ttttcgcaat gtgtatcaga cataaataac
     2641 agaaattaat tttacaatat tcgaatttgc gcagaataaa catatttgat aatgatgtat
     2701 agtattaaaa gctataaaaa actgaagaaa caatttagtt taaacgtaat ccgctccctc
     2761 acagtcgcgg ctcggatgat taattagata attaaatgga attatgacta aaaccgagcc
     2821 acgaccgtaa gggagtggat taaatggaat tataactaaa accgaaagcg gatagatcaa
     2881 acttcaacac caaacactct gtctttattt tcaaaaacag ccattggtaa cccttgttca
     2941 taaacaacgt attgaatcgt tgcttcaatt tcttcctcat tccaaagata gcgagtactt
     3001 ccatggcgag tccatctgtt ctttctatca ccatccagcc ttgtataatt taaataacgt
     3061 gaagcatatg cttttactgt attcataatg tgttcgggag aaatttgggc gtggataaca
     3121 agatgtacat gatttgatct tacatgagca gccagtaata cccattgacg atatctgcac
     3181 acttcacaaa ttgcttgcaa aacaatatga cgccttaatt catccaggga ataaggtgtt
     3241 tccagcatga ggttttttac ctgatataac cgccttcgat cataagggat aaattcagtt
     3301 cctgggatat tattgtagcg atcaactgaa gttgatttgc ctccatgtaa ccatgttcca
     3361 taacaggtaa aggtaataaa ataggcgagt ggaatgactg attcagatat cattatttca
     3421 ccatttccat ttggtttagg acttgtttat gtaagaaaag gttatcacac taactaatta
     3481 tttttgaagt aaattttgct ttcgaaaatt agtaaaataa ccagttttcc gcttcct
//
LOCUS       DBNCWK010000187         3473 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.188, whole genome shotgun sequence.
ACCESSION   DBNCWK010000187 DBNCWK010000000
VERSION     DBNCWK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.188"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1265
                     /locus_tag="AC06IO_002730"
     CDS             <1..1265
                     /locus_tag="AC06IO_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HIO9875892.1"
                     /translation="KITPLLKGHFVFSEINIDGLKLFINQENNEKPLSSEKNANQYNT
                     TTYPNEQFAIQKLLLSHGQIILNNKGHSTVLKNIQIGAEQFNLKNSPFSVQIKAKLTD
                     AAFLQTAKANINFKGRVSLSPSIIDELNSGISKSSIEGQLQIQNILLNQFAIKKINAT
                     LKTHKRDIQFNPLTLSLYNGESIGDMDYVIATQQLLINQTATNLDGKQLITSLLKHPA
                     ISGNLDYSIHASIPLKALSIESLVSKGTITLKDGEVYNINLDQLLNNLKVKLNSLMTE
                     TPDNIKKLTQSADWDQNKNMQGNTKFKLANFKFQLQTGAISSDSFLLQTDKLQVNGEG
                     RINLLNQEINSNIKATLNDNSTDNTLQKIQQALGGYFPLVVSGTVENPIVLPDFKAIS
                     PVLSSLAIKSALTKPLKIIQKPLKELIH"
     gene            1262..2329
                     /gene="mutY"
                     /locus_tag="AC06IO_002731"
     CDS             1262..2329
                     /gene="mutY"
                     /locus_tag="AC06IO_002731"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIO9875893.1"
                     /translation="MSTNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIML
                     QQTQVQTVIPYFNRFIEHFPDIFLLANAEEDEVLSLWSGLGYYSRARNLHNTAKIISD
                     QYNGVFPEDLNILVQLPGIGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPE
                     QAQVKKKLWGLASSCMPNERCADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNK
                     KQHLYPTKKIKKQRPILSQQFLVLHNGQNQVYLEKRPPSGLWGGLWCLPSINNQTCPI
                     EHIQLFYKLQGDSPKLITRFKHSFSHFHLEITALSIRIEPMNNFISESRGQWFTKETL
                     PTLGLAKPTTLILSKLMETGA"
     gene            2541..3272
                     /gene="ceg18"
                     /locus_tag="AC06IO_002732"
     CDS             2541..3272
                     /gene="ceg18"
                     /locus_tag="AC06IO_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg18"
                     /protein_id="HIO9875894.1"
                     /translation="MHLTREVNIMSILSAVSRVINNFSTEIKNRVVEVGSNLKKSTEE
                     FIGEVVIKAAVNRLKQGMLNALSSSNDTTKDNGNDLSTNNPDIEDVSSPTLSTKKKTE
                     ANTTLAGSTGIMQKLLGNIGVNSEQSIEVLSKHFNGAVERFTQDVSKAANEAVCEKIK
                     SIQPEDVIKGINGLIGNTLKVDSESGDTTLSFKLESAVQILGVILAAMGMLMFASQTK
                     STEQKTLEIEHLPTEEIQPPSLAIH"
BASE COUNT         1230 a          653 c          572 g         1018 t
ORIGIN      
        1 taaaaattac tcctttgttg aaaggacatt ttgtatttag tgaaattaac attgatggct
       61 taaaattatt tatcaatcaa gaaaataatg aaaagcctct ctcttctgaa aaaaacgcta
      121 atcaatacaa caccacaacg taccccaatg agcaatttgc cattcaaaaa ttattattaa
      181 gccatggaca aattatcctt aacaataagg ggcattctac tgtcttaaaa aatattcaaa
      241 tcggagcaga gcaatttaat ttaaaaaact cgcccttttc agtacagatt aaagccaaac
      301 tgactgatgc tgctttttta cagacagcca aagctaatat taattttaaa ggtcgagtta
      361 gtctatcacc ctcaattatt gatgaactca attcaggaat tagtaagtct tccatagaag
      421 gccaactgca aatacaaaat attttattga atcaattcgc tatcaaaaaa atcaatgcca
      481 ctcttaaaac tcataaaaga gatatccaat ttaatccact tactttgtca ctctataatg
      541 gggaatcaat aggagatatg gactatgtca tcgctacaca acaattgtta attaatcaaa
      601 ctgcaaccaa tttagatgga aaacaattaa tcacttcttt attaaaacat cctgcaatta
      661 gcggtaattt agattactca attcatgcct caataccatt gaaagcactc agtattgaga
      721 gtcttgttag taaaggaacc attacgctta aagatggaga agtctataat attaatcttg
      781 atcaattact taataatcta aaagtaaaac ttaattcgct tatgactgaa acgccagata
      841 acataaaaaa actgactcaa tctgcggatt gggatcaaaa taaaaacatg caaggcaata
      901 ccaaatttaa attagcaaat tttaaattcc agttacaaac cggagcaatt agcagtgatt
      961 catttttgct gcagactgac aaactccagg tgaatggaga agggagaatt aatctgctaa
     1021 atcaagaaat aaactccaat attaaggcca cgttaaacga caacagtaca gacaatactt
     1081 tgcaaaaaat tcaacaagca ttgggtggct atttcccact ggtagtatct ggcacagttg
     1141 aaaatcccat agttttgcca gattttaaag caattagccc tgtattaagt tcgttagcga
     1201 tcaaatcggc tctgacaaaa cctttaaaaa taatacaaaa accacttaaa gagctaatcc
     1261 attgagtaca aactctttaa atcaactttt cagtcaatta ctgcttgatt ggtatgacct
     1321 gcatggacgc aaagatttgc catggcaatt accccgatct ccctacagag tttgggtatc
     1381 agaaatcatg ttacaacaaa ctcaagttca aactgttatt ccctacttta atcgttttat
     1441 agaacatttc cctgatattt ttttgcttgc taatgcagaa gaggatgaag tgctatctct
     1501 atggtcgggg ttaggctatt acagtcgcgc cagaaatctt cacaacacag caaaaataat
     1561 tagtgaccag tataatggcg tatttcctga agatctgaac attttagttc aactgccagg
     1621 aataggacca tcgacagcag cagcgatact ttcacaagct tttaataaac cagctgctat
     1681 tttagatggg aatgtgaaac gagttttatc tcgatttttt ttaatcgaag gttggccaga
     1741 acaagctcag gttaaaaaaa aactatgggg gcttgcgtct tcttgtatgc ccaatgaacg
     1801 atgtgcggat tatacacaag caattatgga cttaggtgca acatgctgca caaataaaaa
     1861 tcctcagtgt ttgcgttgtc ctgttaaaaa tcattgcctg gcctttcata ataaaaagca
     1921 acatctttat ccaacaaaaa aaataaagaa acagcgccca atattatccc aacagttctt
     1981 ggtgttacat aatggacaaa atcaggttta tctggaaaaa agacctccct cagggttatg
     2041 gggaggctta tggtgtttac ccagtataaa taatcaaact tgcccaattg agcatatcca
     2101 actcttttac aagttgcaag gagatagccc aaagctaatt acgcggttta aacacagctt
     2161 cagccatttt catttggaaa taacagcatt gagcataaga atagagccta tgaataattt
     2221 tatctctgaa tccagaggac aatggtttac aaaagaaacc ttgccaacat taggccttgc
     2281 caaaccaacc accttgatac tatccaaatt aatggagact ggcgcctagc caagaaccgt
     2341 tttaaaacca ccaaaaggtg tataaactgt taagtccgca taagccaata tcaatctaac
     2401 aaaatcttta caaataattt atttattcat tatttaataa ataatgaaca attactttta
     2461 atttacattt ttttgtaaag aaatgtttca atcttaacaa tttcttaatg ttcacttaat
     2521 gttctcttct tattatttat ttgcacttaa caagagaggt aaacattatg tcaatattat
     2581 ctgctgtttc acgtgtcata aataattttt caactgaaat taaaaataga gttgttgaag
     2641 tggggagtaa tttaaagaag tcaacagaag agtttattgg cgaagtagtc ataaaagctg
     2701 ctgttaatcg tttgaaacaa ggaatgttga atgctttgtc ttcttccaat gacaccacca
     2761 aagataatgg taatgattta tccacaaaca accctgacat tgaagatgta tctagcccta
     2821 ctttaagcac aaagaaaaaa actgaggcaa ataccactct ggctggcagt accggtataa
     2881 tgcagaaatt gttgggtaat attggcgtaa attctgaaca aagtatagag gtattgtcca
     2941 agcattttaa tggtgctgtt gagcgtttta cccaggatgt ttcaaaggct gcaaatgagg
     3001 ctgtgtgtga aaagatcaaa agtatacaac ctgaagatgt gataaagggt attaacggtc
     3061 taatcggcaa tactctcaaa gtagactcag agtccggcga cactacccta tcattcaagc
     3121 ttgagagtgc cgtacaaatc ctgggcgtca ttcttgcagc catgggtatg ctgatgtttg
     3181 catctcaaac taaatcaacc gagcagaaaa ctctagagat agaacatctg ccaactgaag
     3241 aaattcaacc accttcattg gctattcatt aataaatgct cacggcttgt tcttcaaggc
     3301 tgcatgccgc aaatcattct tggtatgtag cttgaagaat tggcaacaca ttcataaact
     3361 ccttaattac aacgtttgat cactgaactc gccccgcttc atgatataca agcatttttt
     3421 aattaacatg atgattacat gagctcctgg caaaacctga atataaagtg aca
//
LOCUS       DBNCWK010000188         3440 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.189, whole genome shotgun sequence.
ACCESSION   DBNCWK010000188 DBNCWK010000000
VERSION     DBNCWK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.189"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(117..392)
                     /locus_tag="AC06IO_002733"
     CDS             complement(117..392)
                     /locus_tag="AC06IO_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875895.1"
                     /translation="MKEKHFAYKITLSSTGLVCTHSEIEENKMIDLLKEIKSDDSQAI
                     FWIFKQFDKQPKEQLGIIDCSKNRIYYHYSGEVEEIDEAIEKLSKKI"
     gene            666..1799
                     /locus_tag="AC06IO_002734"
     CDS             666..1799
                     /locus_tag="AC06IO_002734"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIO9875896.1"
                     /translation="MISSKHYKESMTLLVEGSLRAIPFNIILATLLAIDLVYNNVPAL
                     IIGVWIFAIVFISLVRSLFCWKIIKRGVEQNKIQSTLLKFFLLTFVMGSIWGACYFIT
                     LPYLNDLHEFIIILVFGGMCAGAIASLSVYLPAYYAYVFPMLIPVIIYNYALLDLDRT
                     ILATMFLIFIAMLIISARINNRLLNQNFRLFNEKELLISELKVMTITDSLTGLYNRRY
                     FDTLFPQEFNRARRNQYFLSLVSIDADNFKLINDNLGHPFGDKVLISIADLLKKIFRR
                     SNDILFRLGGDEFAIIIANQPIKDTISICQSIKNPFKTENLNHNYGSNEQLLMSHVTL
                     SIGIVYIHFESSVSIEHAITAADKALYQAKKKGKNQIVVKKLL"
     gene            complement(1861..2502)
                     /locus_tag="AC06IO_002735"
     CDS             complement(1861..2502)
                     /locus_tag="AC06IO_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875897.1"
                     /translation="MKMKLQLIAIGILLSINVFADGYYPEPTSQDQPWTIIASFGSGR
                     YQHMYSKDGKTALGRLALANELMLSGDIALGLELGVQNGNKMRLNIPRETLSVLGWLP
                     VETNLSPMLDLLVTAKSDPLFGSSFFAQLKGGVAYRSWQVDLYPINEISQLAGEIQAG
                     FGYPITALASLSLLYQGVFGNDPNVKLNMSSKTGSVSNIPVLHGILLGFTVNL"
     gene            complement(2675..2762)
                     /locus_tag="AC06IO_002736"
     tRNA            complement(2675..2762)
                     /locus_tag="AC06IO_002736"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2726..2728),aa:Ser,seq:gga)
     gene            2985..3419
                     /locus_tag="AC06IO_002737"
     CDS             2985..3419
                     /locus_tag="AC06IO_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875898.1"
                     /translation="MTLQHSIQKGVSDFSFIIGTGLKYGGAGGLLVMAVVGTVAIDLV
                     LLAAAEKHHNSFLTGFVLGSMFSRGNLDPVPLLIASPITSAVAVVLSVALGVPGVGLA
                     ILAGWTLAGTLLAVGMGLEAFAKSIDPELSSESDYNSCLCFN"
BASE COUNT         1046 a          575 c          652 g         1167 t
ORIGIN      
        1 tcatattatg gtgtgtttgt tttcgtatga ctttgtgcac tatattttca atccatgcgt
       61 ttaaatattg ttgcattcga tgaaaataaa tctgtctatt gtagctactt ttggatttat
      121 atttttttac ttagtttttc aatagcttca tcaatttctt caacttcacc agaataatgg
      181 taataaatac ggtttttgct gcaatcgatg atccctaatt gttctttagg ctgcttatca
      241 aactgcttaa agatccagaa aatggcttgg gaatcatcgc tttttatttc ttttaacaga
      301 tcaatcatct tgttttcttc aatttcagaa tgtgtacaaa ccaggcctgt tgaagaaaga
      361 gtaattttat aggcgaagtg tttttctttc atgatgatac tccatttaat tcatcatctt
      421 aaaaaccatt gtaaaaacaa actataaagc ttaactgatt aaattatagt ctctaataaa
      481 taagaaggat aaaaaattat gaacagatta atacagaaaa gaatcgaacg attgatgaat
      541 accaaaaaaa gtataaaaag acaccaataa cacagaataa agggcaatat atgttaagac
      601 tctatgagaa tctcaatgta ctataatttt aaagagtgga tattaattta tggacattgg
      661 ataaaatgat ttcatccaag cattacaagg aaagtatgac cttgctggtt gagggatctc
      721 taagagcaat tccgttcaat atcattttgg ctacattgct tgccattgac cttgtttata
      781 ataatgtgcc tgcattaata ataggggtat ggatttttgc tattgtcttt ataagcttgg
      841 taagaagtct tttctgttgg aaaataatta agcggggagt ggaacaaaat aaaatacaat
      901 ccactttgct caagtttttt cttcttactt ttgtcatggg cagtatatgg ggggcgtgtt
      961 acttcattac tttaccttat ttaaatgacc ttcatgaatt cattattatt ttggtatttg
     1021 gcggaatgtg cgcaggcgca attgcctctc tctctgttta tttgccagct tattacgcct
     1081 atgtttttcc aatgcttata ccagtcatta tttataatta tgccttgtta gatctcgata
     1141 gaacgatttt agcaaccatg tttctgattt ttattgctat gcttattatt tcggcaagga
     1201 ttaataatag attactcaat caaaattttc gattgttcaa tgaaaaagaa ctattaatta
     1261 gtgaattgaa agtgatgaca ataactgatt ctttgacagg cttatataac aggagatatt
     1321 ttgacacgct attcccgcag gagtttaatc gagcaagacg taatcagtat tttttaagcc
     1381 tggtatcaat agatgcagat aattttaaat taatcaatga taatttggga catccttttg
     1441 gtgataaagt ccttatcagt atcgctgatt tattaaaaaa aatatttcga cgttcgaacg
     1501 atatactgtt tcgtcttggg ggagatgagt ttgcaataat tatcgctaat cagccgatta
     1561 aagacacaat atctatttgt caatcaatta aaaatccttt taaaacagaa aacttaaatc
     1621 ataattatgg ttcaaacgag caattactga tgagtcatgt caccctaagc atagggattg
     1681 tttatattca ttttgaaagt tcagtcagta tagagcatgc aattactgcg gcggataagg
     1741 cattatatca agctaaaaaa aagggaaaaa accaaattgt agtaaaaaaa ctactttaag
     1801 aagatgacat tctatcttta actgagatgg cgcctggaag ctgcgtccta gtttaatgga
     1861 ttataagttg acagtaaatc ctaataaaat tccatgtaaa acagggatat ttgaaacact
     1921 gccagttttt gagctcatgt ttaatttgac gttaggatcg ttaccaaaaa caccctgata
     1981 cagcaaactc aaactggcca aagcggtgat aggataacca aagccagctt gaatttctcc
     2041 ggctaattgt gaaatttcat tgatagggta gagatcaact tgccaagacc tgtaagcgac
     2101 accacctttt aattgtgcaa aaaaagaact tccaaataaa gggtcgcttt tggcggtaac
     2161 taataaatcc agcataggac tgaggttggt ttctaccggt agccatccaa ggactgataa
     2221 ggtttctcgt gggatattca agcgcatttt attgccgttc tggactccaa gttctaatcc
     2281 cagagcaata tctccactca gcattaattc attagccaga gccaatcggc ctaaagcggt
     2341 tttgccatct tttgagtaca tgtgctggta tctgccactt ccaaaactgg caataattgt
     2401 ccatggttgg tcttgagagg tgggctcagg atagtaacca tcagcaaata cgttgattga
     2461 taataaaatt cctattgcaa ttagttgtaa tttcattttc atctttcata tccctgattg
     2521 aattggttgg ttctgaaagc agttttacca aaagaatctt atatgagcca gctgtagtct
     2581 ttacacaatg gatataattt actgctttat gcaactcaat gaaagcggca attatagcct
     2641 tataccctgt gaaggataac caggaattgt aatttggcgg agagagaggg attcgaaccc
     2701 tcgatacgtt gccgtataca cactttccag gcgtgcgcct tcgaccgctc ggccatctct
     2761 ccaaccgggc aagcttatac ttgtcggcaa ataaaagcaa gactgtgata attattaaag
     2821 aggttttcca tcacaaagat aatatattct atttttggat gggaataaat tgtatcgctt
     2881 tatttgagtt ttaatccaat ctactcgtta cttcagtgtt caattagtgt tgattatgtg
     2941 caaaattaag tgtattattc ctcaattttt taggagtaag attaatgaca ctgcaacatt
     3001 ccatacaaaa aggggtatct gattttagtt ttattattgg cacaggtcta aagtatggtg
     3061 gtgctggcgg tcttttagtg atggctgttg taggaacggt tgctattgat cttgtcttgc
     3121 ttgctgctgc agaaaaacat cataacagtt ttttgacagg gtttgtgtta ggttcaatgt
     3181 tttccagagg taatctggat ccggttccac ttttgatcgc atcccccatc acatcagctg
     3241 ttgctgttgt gttatctgtt gccttgggtg ttcccggggt cggtcttgcg atactggctg
     3301 gatggacttt ggctggcact ttattagctg ttggtatggg cttggaagcg tttgccaaat
     3361 ccattgatcc tgaactttca tctgagtcag attacaattc ttgtttgtgc tttaactaat
     3421 agagtcaaga ggggaggtca
//
LOCUS       DBNCWK010000189         3416 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.190, whole genome shotgun sequence.
ACCESSION   DBNCWK010000189 DBNCWK010000000
VERSION     DBNCWK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.190"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            112..453
                     /locus_tag="AC06IO_002738"
     CDS             112..453
                     /locus_tag="AC06IO_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875899.1"
                     /translation="MSMTLSLGQKYGSFFWGCTKFAVSPYTNTFLSIVKSKEVEEADC
                     LSKALAASIVCGVLTFVVPVLPVLCAFTFTLSSIAMLLAVASMFVTYPIAILGDALCG
                     PELSEDYHYSF"
     gene            674..1204
                     /locus_tag="AC06IO_002739"
     CDS             674..1204
                     /locus_tag="AC06IO_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875900.1"
                     /translation="MSKFPHKNPETLRLYFQGKNLEQLIEINRSYGPHFENLENTIDR
                     LTQEISTQKGKLSGLLKANEELSQSYDKIVVEQERFESNRSSIMSDSCTGAERYLALS
                     ALGMSPLDCYHSNSRSLQNEIDTTNKKLKELNSHLALWKNQRSKAVSELKILNPIIDE
                     KRRELEVAPQFTLSSN"
     gene            complement(1297..1665)
                     /locus_tag="AC06IO_002740"
     CDS             complement(1297..1665)
                     /locus_tag="AC06IO_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875901.1"
                     /translation="MQTTKAHYSKIFFALFLFFSTGSSSLFACPCFNMGFLHVAFLDD
                     KDTECRIYKEGSVVYKIEIFDKEKIASSTHTQCTLNTEHHDVFMNYDFHYLDDHGDCI
                     REILEACNKLKINSLHMDDE"
     gene            1756..1935
                     /locus_tag="AC06IO_002741"
     CDS             1756..1935
                     /locus_tag="AC06IO_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HIO9875902.1"
                     /translation="MLSWALIFFIIAIIAAAFGFGGIAVAAAGIAKILFFLFLVMFVI
                     FLIMGLIGRRGPPPV"
     gene            complement(1976..3106)
                     /locus_tag="AC06IO_002742"
     CDS             complement(1976..3106)
                     /locus_tag="AC06IO_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HIO9875903.1"
                     /translation="MKEKYIGLHKIELNTPCLVIDYEKLKYNLKMMQQHGFKWGIGIR
                     PHCKTHKCTQIARLQLEYGAIGISAAKLSEAEVLVSKQITNVLITSPIVSEAKHSRLF
                     NSLKSAPDLMLVVDGKDNIRMLNELGKSLNQPVYVLLDIDSGIGRTGINPEAALEYGH
                     FINGMNWLKLKGIQCYAGHLQHIQNYEERKALSLRIMEMASRIVKSFREANLPCEILT
                     GTGTGTFDIDVEASEVTEIQPGSYAVMDVEYGSIGSKNNPSSFQLFKNSMTLLTSVIS
                     SNRREHATVDAGTKSIYVDPHHKPHIISHPRLVYDWSGFGDEHGKVFSCDGGEHFLQN
                     GDVLELIVPHCDPTINLYDKFYITENNVVIDIWDIDMRGKSQ"
BASE COUNT         1078 a          642 c          570 g         1126 t
ORIGIN      
        1 aaaatagata aaaatagaac aaatttatta atttaatgct tatttttata ggttttaatc
       61 tgaatttgaa ttataattgc tgacctaatt taagttaaat tggagtaaaa aatgtcaatg
      121 actctctcgt taggtcaaaa atacggctca ttcttttggg gatgtactaa atttgctgtt
      181 tccccctata ccaatacttt tctatctatc gtcaaatcga aagaggttga agaagcagat
      241 tgtttatcca aggctcttgc tgccagcatt gtctgcggag ttcttacttt tgttgttccg
      301 gtattaccag ttctatgcgc ttttacgttt accctgtctt caatagccat gttgcttgcg
      361 gtagccagca tgttcgtgac ttatccgatt gcaatacttg gtgatgcact gtgcggacca
      421 gaattgtctg aagattacca ttattctttc taatcagttt taagataatt tcatttgaaa
      481 tcatcataag tatcaacaat caggaagagt atttttaaac tcgtaaaaaa tactcttcct
      541 gattaatatt gatcaaaaat aaactccgcc atcaacgaaa gcccttaatt tactcactgt
      601 gaagtatatg tgtttgatat gatcaaaaag catatgataa gatatgccga ttttgattca
      661 aaaaggtaaa aacatgtcta aattcccgca taaaaaccct gagacacttc gtctatattt
      721 ccaaggaaaa aacctggaac aattaataga aattaatcgc tcctatgggc ctcattttga
      781 aaatctggaa aacacgattg ataggctaac tcaagagata tctactcaaa aaggaaaact
      841 atccggactt ttaaaggcaa atgaagagct ttcccaaagt tatgataaaa ttgtagttga
      901 acaagaacga tttgaaagca atcgctcatc gattatgagc gattcttgta ctggagcaga
      961 acgctattta gcactcagtg ctcttgggat gtcaccatta gattgctacc atagcaatag
     1021 caggagttta caaaatgaaa tcgacacgac aaacaaaaaa ttaaaagaat taaattccca
     1081 tcttgctttg tggaaaaatc aaagatcaaa agcagtttct gaattaaaaa tattaaaccc
     1141 tattatcgat gaaaaaagaa gagaactcga agtcgctcct cagtttacat tgagttcaaa
     1201 ctaaaaattg ataagcctaa cccgggttaa tcttaattat aatccgggcg agcgtcctaa
     1261 tcattcaata ccagtcctat ttcaccttgc tttatcttac tcatcatcca tatgcaagga
     1321 gtttattttc agtttgttac atgcctccaa tatttctctg atacaatccc catgatcatc
     1381 gagataatga aaatcataat tcataaaaac atcatgatgc tcagtattca gcgtacattg
     1441 ggtatgggta cttgacgcaa ttttttcctt atcaaaaatc tcgatcttat aaacaacact
     1501 tccttcttta tatatgcgac attccgtatc tttatcatcc aaaaaggcta cgtgtaaaaa
     1561 gcccatgtta aaacaagggc aggcaaataa tgagcttgaa ccagtagaaa aaaaaagaaa
     1621 taaggcaaag aatatcttgc tgtagtgagc tttggtggtt tgcatattct tccctgatta
     1681 agtgtctttt gtctaattgt agaccattat tagtctataa tttaatgaac aggataatct
     1741 ttacagggag ctgttatgtt aagttgggca cttatttttt ttatcattgc aattattgca
     1801 gccgcgtttg gatttggcgg cattgcagtg gctgccgctg gaattgcaaa aatattattc
     1861 tttctatttt tggtgatgtt tgtcatattt cttattatgg gattaatcgg gcgtcgtggc
     1921 cctccccctg tttagcaaaa atatgaaact caaacacctg gttgaatagt atagattatt
     1981 gtgactttcc acgcatgtcg atatcccaaa tatcgataac gacgttattt tctgtgatat
     2041 agaacttatc atataggttt attgtcggat cacaatgagg aacaataagc tctaaaacat
     2101 caccattttg cagaaaatgt tcccctccgt cacaagaaaa taccttgcca tgctcatctc
     2161 caaatccact ccagtcataa accagtcgtg gatgactgat aatatgtggt ttatgatgag
     2221 gatcgacata aatcgatttc gttcctgcat caacagtggc atgctctcta cgattactgc
     2281 ttatcacgct cgttaaaaga gtcatcgagt ttttaaatag ctgaaagctt gatgggttat
     2341 tttttgaacc aattgaacca tactcaacat ccattacagc atatgagcca ggctggattt
     2401 ccgttacttc acttgcttcg acatcaatat caaaagtacc agttccggtg ccagttaata
     2461 tttcacaagg gagattggct tctctaaaac tcttaacaat cctgctggcc atttccataa
     2521 tacgtagaga aagcgctttt ctctcttcat agttttgaat atgttgcaag tggcctgcat
     2581 aacattgaat tccttttagc ttaagccaat tcatcccatt gataaaatga ccatattcca
     2641 aagctgcctc gggattaatg cccgttcgac caatacctga atcgatatca agcaagacat
     2701 aaactggctg gtttagcgac tttccaagct cattaagcat gcgaatatta tccttgccat
     2761 ctacaaccag cattaaatca ggcgctgatt taagagaatt gaataatctt gaatgcttgg
     2821 cctccgatac aatgggtgaa gtgattaaga cattcgtgat ttgtttggat accaatactt
     2881 cagcctctga tagtttggcg gcagaaatcc ctattgcccc atattcaagt tgtaaacgtg
     2941 cgatttgagt acatttatga gttttacaat gcggccttat tcctataccc catttgaaac
     3001 catgttgttg catcattttt aagttgtatt tcagcttttc ataatcaatc acaaggcatg
     3061 gggtatttag ttctatctta tgaagcccga tgtatttttc tttcatgaga taattcatca
     3121 caatgtcaaa taaatgcgtt ttatgacata aattatttag gtttgccaca atttaagctt
     3181 ttattaaact attttttaaa agatgagcat cctaatgctc aaagatcaat ataaaattgc
     3241 atgactaact gctatgagct ggctcctcga tttcaatgat tttataattt atgtgttatt
     3301 tatttcttac attaaccacc atttttctct gattcaaatc attctatttt gtgattaaaa
     3361 aataggcaac gtccctgaag tctttttaga caagatttaa tcatgaattg agctcg
//
LOCUS       DBNCWK010000190         3400 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.191, whole genome shotgun sequence.
ACCESSION   DBNCWK010000190 DBNCWK010000000
VERSION     DBNCWK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.191"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            18..2243
                     /locus_tag="AC06IO_002743"
     CDS             18..2243
                     /locus_tag="AC06IO_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HIO9875904.1"
                     /translation="MAESKLEIVNKTEREESVILDGNGILRCYNMFPTQFGSIEEMID
                     YLPQLQAMGFNAVWINPVQTAGDVQGLFKRDKTIGVRAYNEVTRSLYAMTDCDLISPY
                     FNPLPPNVDPKLKKQLDMEAMQKFTSEARNNGLVPMFDLVLNHVAADSRHRKEHPEWF
                     QKDVHPDFKDAIAFDYSDPQIRDEIIEQFWKPYIYKYMIDYGFEGVRIDAVGYLNPEL
                     RKQIYAYIYDLAEQFNKPKPVILDELLFSGSQKLTDVVDGLLLPEKGPTHITRGTYYA
                     QRDGYGGLPGWCKEEEGVKAQVVFLDKQKKLHPNAKGGCIAFSGNHDHNSLAMTILEE
                     MAEHRLAKHHTLHGAQEKFKHERSYDYDEALESVLRYSFVKDIQNEILSGNEATIKEV
                     ETKMREKIAFCALTSSGGWYALSGDETGDLLAKPVFRRVHALDQTYYAQRTHIIFDKK
                     YPDNKVAMEVLTEMAIENIQKEKAGSIYNSLQHDKQSQERLLVPYLETLQNQINCGDT
                     VVCKAFQEKLAQKNITIGFTDENYSPDPRTPQNGWNGQHDMRKFMFEINSILSSLPSS
                     NMGFWSEVIPIQSKPGLLAVVRKNGLGLSSDTDIAIVNMDPSQKVELTRDDIHQLACN
                     FQKRVISEYTYYNDGKDKQYNWHTNNPDFNMAYHTVMSCIEAKRIHVDDSIKTNFPVH
                     SANPYGMFSKKQSSKMNIAKIAKMSGEDTTLIEKGTNDTEGIIHDDEQIQPTITVENK
                     K"
     gene            complement(2405..2596)
                     /locus_tag="AC06IO_002744"
     CDS             complement(2405..2596)
                     /locus_tag="AC06IO_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875905.1"
                     /translation="MTENKEEDYWFTNAAKGILVTNAYAISESSSESYQSELEQRISK
                     LITPPGNTNEEEQQKIYSP"
     gene            2789..3124
                     /locus_tag="AC06IO_002745"
     CDS             2789..3124
                     /locus_tag="AC06IO_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIO9875906.1"
                     /translation="MDKSTLRQRARNLRQNSTDAERHLWYHLRANRLGFKFRRQVLIG
                     SYIVDFVCIEKRLIIELDGSQHMDNQIYDTKRSDWLMTHGVKVLRFWNNDVFQQTPSV
                     LEVITSALF"
     gene            complement(3116..>3400)
                     /locus_tag="AC06IO_002746"
     CDS             complement(3116..>3400)
                     /locus_tag="AC06IO_002746"
                     /inference="COORDINATES: protein motif:HMM:NF013752.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIO9875907.1"
                     /translation="GPRSIVSFFLADKAYDIDGFRHALAEDGIKPVIPSKKNRLNPTT
                     HDRHIYKERNIVERFFQKIKRFRRIATRYDKTARMYLTGVMFVSIILALK"
BASE COUNT         1094 a          592 c          708 g         1006 t
ORIGIN      
        1 gatatgggca tattattatg gcagaatcaa aattagagat agtcaataaa acagaacgtg
       61 aagaaagtgt aatcctggat ggtaatggga ttttacgttg ttataacatg tttcctactc
      121 aatttggaag catagaggag atgattgact atcttcctca attacaggcc atgggattta
      181 atgctgtatg gataaatcct gtacaaacag caggggatgt tcagggttta tttaagcgtg
      241 ataagacaat tggcgtcaga gcttataatg aggtgacaag aagtctatat gctatgacag
      301 attgtgattt gataagtcct tattttaacc cgctccctcc caatgttgat cctaagttga
      361 aaaagcagct tgatatggaa gcaatgcaga aatttacatc agaagcccgc aataatggtt
      421 tggtaccgat gtttgatttg gttttaaatc acgttgccgc cgattcacgg cacagaaaag
      481 aacatcctga atggtttcaa aaggatgtcc atcctgattt taaggatgct atcgcgtttg
      541 actacagtga ccctcaaatc cgagatgaaa ttattgaaca attttggaag ccttatattt
      601 ataagtacat gattgattat ggctttgagg gggttcgcat tgatgctgta ggctatttaa
      661 atccagaatt acgcaaacaa atttatgctt atatttatga tcttgcggag cagtttaata
      721 aacctaaacc cgttatattg gatgaacttc ttttcagtgg gagtcagaag cttaccgatg
      781 tggttgatgg tttgttgctt ccagaaaaag ggccaaccca tattactcgc ggtacctatt
      841 atgcgcaaag agatggttat ggtggtttgc ccggatggtg taaggaggag gagggagtta
      901 aggctcaagt tgtatttctg gataaacaaa agaaattaca tcctaatgcc aaaggaggat
      961 gtattgcttt ctcgggcaat cacgatcaca actccctagc catgacgata ttggaagaaa
     1021 tggcagaaca ccgtttggca aaacatcata ctttgcacgg tgcgcaagaa aaatttaaac
     1081 atgaaagaag ctatgattat gacgaggccc tggagtcagt ccttcgctat tcttttgtga
     1141 aagacattca aaacgagatt ctaagcggta atgaagcgac aatcaaagaa gtagaaacaa
     1201 aaatgcgtga aaaaatagct ttctgtgctt taacatcaag cggcgggtgg tatgccctat
     1261 caggtgatga aacaggtgat ttattggcaa aacctgtatt cagaagggtt cacgctctgg
     1321 accaaaccta ttatgcacaa cgtactcata taatctttga taaaaaatat cccgacaaca
     1381 aagtagctat ggaggtatta accgaaatgg ctatagagaa tatccaaaaa gaaaaagctg
     1441 gaagcatcta taacagttta cagcatgata aacaatcgca agagcgattg ctcgttcctt
     1501 atctggagac tttgcaaaat caaattaatt gtggcgatac agttgtttgc aaagcattcc
     1561 aagaaaaact tgcccaaaaa aatataacga ttggttttac agacgaaaat tatagtccgg
     1621 atcccagaac acctcaaaat ggttggaacg gccaacatga tatgaggaaa tttatgtttg
     1681 aaatcaatag catattaagt tccttgccga gttccaatat ggggttttgg agtgaagtta
     1741 tcccaatcca gtcaaaacca gggttgcttg cagtagtacg taaaaatggg ctaggcttaa
     1801 gttctgatac tgatattgcg atcgttaata tggatccaag tcaaaaagtc gagttaacaa
     1861 gagacgatat tcatcaatta gcctgtaatt tccagaaacg agtaatttct gaatatactt
     1921 attacaatga tggtaaagac aaacagtata attggcatac aaataatcct gattttaata
     1981 tggcttatca tacagtgatg tcgtgtatcg aagccaaaag gatacatgtt gatgattcga
     2041 ttaaaactaa tttccctgtt cactcagcaa atccctatgg catgtttagc aaaaagcaat
     2101 catcgaaaat gaatatagcc aagatagcaa aaatgtctgg ggaggatacc actttaattg
     2161 aaaaaggtac taatgatact gagggtatca tacacgatga tgagcaaatt caacccacaa
     2221 taactgtaga aaacaaaaaa taaagaagta ctgtaataaa agtggtttca acttggtgac
     2281 cgggtattat tttgataaat cggatactat gaaatataac taattacaaa gaccgtataa
     2341 atagtaagtt ttaggttcat atgaattgat ttaatttcta agtcatacct atttcatcta
     2401 acaactaggg gctgtatatc ttttgttgtt cttcttcatt tgtattgcca ggaggtgtaa
     2461 ttagtttgct gattctctgt tctaattctg attggtagga ttcacttgag ctttcggaaa
     2521 tagcataagc gttagttaca aggattcctt ttgcagcatt agtaaaccaa taatcctctt
     2581 ctttgttttc tgtcacagat tttgatcata gagatttaac attttttctt ttaatcacta
     2641 ggcaacgtcc ctgaagtttt tttagacaag atttaatcat gaattgagct cggattttca
     2701 acttcaccag gatcttcccc cctctcccgc gctaggagct ttgatagtag gatggtgcct
     2761 ttcgcgggag agggttgggg agagggaaat ggacaaatct acgctaagac aacgagctcg
     2821 caatttgcga caaaatagca cggatgccga gagacatctt tggtatcatc ttagggcaaa
     2881 tagactggga tttaaattca ggagacaagt gctcataggt tcatacatcg tcgattttgt
     2941 ctgtattgaa aagcggttga ttatcgagct tgatggcagt caacacatgg acaatcaaat
     3001 ttacgataca aagcgcagcg attggttaat gacacatggg gttaaggtgt tgcgtttttg
     3061 gaataacgac gtttttcagc aaacgccatc tgttctcgaa gtcatcacat ctgcgctatt
     3121 ttaaagccaa tattatactt acaaacatta cgccagtgag gtacatcctg gctgttttat
     3181 catatcttgt agcaatcctt ctaaagcgct taatcttctg aaaaaagcgc tcaacaatat
     3241 ttctttcttt gtagatatgg cgatcatggg tcgttggatt caggcggttc tttttgcttg
     3301 ggataactgg ctttatccca tcttccgcta gagcgtgacg aaaaccatca atatcgtaag
     3361 ctttatcagc caaaaaaaaa ctcacaatac tccttggccc
//
LOCUS       DBNCWK010000191         3319 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.192, whole genome shotgun sequence.
ACCESSION   DBNCWK010000191 DBNCWK010000000
VERSION     DBNCWK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.192"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            144..2168
                     /locus_tag="AC06IO_002747"
     CDS             144..2168
                     /locus_tag="AC06IO_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIO9875908.1"
                     /translation="MELRIDNTPFKAIFQPLDLGFTQIKNRLLMGSMHTGLEEDKENL
                     NRLASFYRERAQGGVGLIVTGGFAPNRAGRLAPFAAKLTNSKEQHRHEVITQTVHEAG
                     GKIALQILHAGRYGYHPFIVAPSGIKSPISPFKPWEMSKRRIIKTIKHFARCARLAQL
                     AGYDGVEIMGSEGYLINQFIVTHTNHRRDEWGGSFSNRIRFPIEIVRSVREAVGEHFI
                     IIYRLSMLDLVAEGSNWQEVVTLAKAIEQAGASLINTGIGWHEARIPTIGTMVPSAAF
                     TQVTQHLKPEIKIPVITSNRINTPELANQLLEDGVADMISMARPFLADPCFAEKAKLG
                     ESKAINVCIACNQACLDQVFVNKTASCLVNPRACNETTLVYETTNQPKAIAVVGSGPA
                     GLAFAVTAAERGHKITLFEKANQIGGQFNLAKKIPGKEEFQHTLNYFAYQLQKYKVDV
                     RLNTEATVELLKDYDEVVLASGIIPRVPEIKGIDHKKVVSYIDVITQNKPVGSRVAVI
                     GAGGIGFDVAEFLTHDHQLTSEQFYNEWGIDISGQHRGGLKPAEIAPSPREVYLLQRK
                     KEKMGKRLGKTTGWIHRSSLKHKQVKMLSGVNYERIDDQGLHLTIDDKPQVLEVDSIV
                     ICAGQIELKNLYEPLKMAGKLVHLIGGAYKALELDARHAIDQACRLAALV"
     gene            2165..3082
                     /locus_tag="AC06IO_002748"
     CDS             2165..3082
                     /locus_tag="AC06IO_002748"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIO9875909.1"
                     /translation="MSKIAVVINPIAGGGRGKKVWKVLKPGLHALFEQTVYRISNRVN
                     DLEAITDGLLAENPDSLLIIGGDGTLNHVLNGLIEKDKLKNPQTKIAFFNAGCGGDFT
                     RQFPQQKMTEFLDRLSHNQFIKCDIGKITLANQVSRYFINIASCGLSGHVVLRVSKSK
                     WLKKLGGTLNYLLHALTGLMTYRKTKVRVQIDDALPFDSSILLMAVCSGQYFGGGMHV
                     APMAKVNDGLFDVVSFNQFSKLNALLKLYKIYSGGHLLDNNVHYVQTKKIKIEPLEES
                     KIEIEADGEIIGCLPAQFELIKETLPLII"
BASE COUNT         1044 a          564 c          743 g          968 t
ORIGIN      
        1 tgtagaccat gagagtttcg taaactctat tttaaagttt agagagacga ttacaaatta
       61 attgaatcgg taaaaaatga ttgctgtata ataagcaagg tttatacttt atcttttaag
      121 ctttaattaa acagagagtc ctcatggaat tgagaattga taatacccct tttaaagcca
      181 tttttcagcc tttggatctg ggctttaccc aaattaagaa tcgtttattg atgggttcaa
      241 tgcacactgg acttgaggaa gataaagaaa atttaaatcg cctggcaagc ttttatagag
      301 aaagagctca aggtggagtt ggattaatcg taacgggtgg ttttgctcct aaccgagctg
      361 gtcgtctggc ccccttcgct gccaaactga ctaatagtaa agagcaacat cggcatgaag
      421 tcattactca aacggtccat gaagcaggtg gaaaaattgc cttgcaaata ttgcatgcag
      481 ggcgttatgg ttatcaccct tttattgtag cgccgagcgg cattaaatcc cccattagcc
      541 cgtttaaacc gtgggaaatg agtaaaagac gaattatcaa aactatcaaa cattttgccc
      601 gatgtgccag actggctcaa ttggctggct atgatggggt tgagatcatg ggaagcgaag
      661 gatatttgat aaaccagttc atagtcactc atactaatca tcgaagggat gaatggggag
      721 gaagtttctc caatcgcatt cgatttccta ttgaaatagt tcgcagtgtt cgtgaggcgg
      781 taggcgagca ctttattatc atttatcgct tgtccatgct agatttagtg gctgagggga
      841 gtaattggca ggaagtggtt accctcgcta aagctattga gcaagctggt gccagtttaa
      901 tcaatacggg tataggttgg catgaagcaa gaattccaac gataggaacg atggttccat
      961 ctgctgcctt cactcaagtg acccagcatt taaagccgga aattaaaata cctgtaatta
     1021 catctaaccg aattaataca ccagaattgg ctaatcaatt attagaagat ggggtcgcag
     1081 atatgatctc catggccaga ccatttctag cggatccctg ctttgcagaa aaagcaaaat
     1141 taggcgaaag caaagcgatt aatgtatgca tagcttgtaa tcaagcatgt cttgatcaag
     1201 tttttgtgaa caaaacagca tcttgcctgg ttaatccccg cgcatgcaac gagacaacct
     1261 tggtttatga aacaacaaat caaccgaaag ctattgctgt agttggttct gggccagctg
     1321 ggcttgcttt tgctgtaaca gccgccgagc gagggcataa aattaccctg tttgaaaaag
     1381 cgaatcagat tggcgggcag tttaatttag cgaaaaaaat tcctggaaaa gaagagtttc
     1441 agcatacctt gaattatttt gcctaccagc tccaaaagta taaagtggat gtacgtttga
     1501 atactgaagc tactgtggaa ttacttaaag actatgatga agtggtcttg gccagtggaa
     1561 ttatacccag agttcccgaa attaaaggca tagatcataa aaaagtggtg agttatattg
     1621 atgtgattac tcaaaacaaa cccgttggct cacgagttgc cgtcattggg gcaggaggaa
     1681 ttggttttga tgtcgctgag tttttaacac atgatcatca attgacttcc gagcaatttt
     1741 ataatgaatg gggaattgat attagcgggc agcatagagg tggattaaaa ccagctgaaa
     1801 ttgcaccgag ccctcgtgaa gtgtatttat tacaacgcaa aaaagaaaaa atgggtaaac
     1861 ggttgggaaa aactaccgga tggattcatc gctccagttt aaaacataaa caagtcaaaa
     1921 tgctttcggg tgtaaattac gagcgtattg atgatcaggg attgcacttg actattgatg
     1981 acaagcctca agttttggaa gttgattcta tagtgatttg cgcaggccaa atagaattaa
     2041 agaatttgta tgagcctctg aaaatggcag gcaagttggt tcatttaata ggaggcgctt
     2101 ataaagcctt ggagcttgac gcacgtcatg ctattgatca ggcttgtcgg ttggctgcat
     2161 tagtatgagt aaaattgctg tagtgataaa tcctatagca ggcggcggtc gcggtaaaaa
     2221 agtttggaaa gtcctaaaac ccgggttaca tgccctattc gaacaaactg tttacagaat
     2281 atctaacagg gttaatgatc ttgaagcaat cacagacgga cttcttgctg aaaaccctga
     2341 cagcttgtta ataatcggtg gagatggcac tttaaatcat gttttgaatg gcttaattga
     2401 gaaagataaa ttaaaaaatc cgcaaacaaa aatcgccttt tttaacgcag gttgtggcgg
     2461 tgattttaca agacaatttc cccaacaaaa aatgaccgaa ttcctggata gattaagtca
     2521 taaccaattt atcaagtgtg atattgggaa aattactctt gccaatcaag tgtcgaggta
     2581 ttttattaat attgccagct gtggtctgtc agggcatgtg gttttgcgtg tatctaaaag
     2641 caagtggtta aaaaaactgg gcggtactct gaattacttg ttgcatgctt taaccgggtt
     2701 gatgacctat cgtaaaacca aagtaagggt tcagatagac gatgctctac catttgatag
     2761 ctccatcctg ctcatggccg tctgcagtgg ccagtatttc ggcggaggta tgcatgtagc
     2821 accaatggca aaagtgaacg atggcttgtt tgatgtggtt tcttttaatc aattttcaaa
     2881 actaaatgca cttttaaaat tatataaaat ttattcagga gggcatttgt tggataataa
     2941 tgttcattat gttcagacta aaaaaattaa aatagagccg ttggaagaaa gtaaaattga
     3001 aattgaagca gatggtgaaa ttattggttg cttaccggca cagtttgagt taattaaaga
     3061 aactcttcct cttattattt aaaaataaag gtcaaaatag ggctcattct ataaaattat
     3121 tttttatcaa gacaggttgt tgtctcaaga cgagatcaca tcatttagtg caataaacaa
     3181 cacagacgat tgttcatatt ttgtcattct gcgcaggcgg gaagccattt ttattgtaag
     3241 ctgtggagca aaatttgaaa tagattttta cttgcgtggg aatgacacat gtgatgtatt
     3301 atttagtcaa aatatgatt
//
LOCUS       DBNCWK010000192         3317 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.193, whole genome shotgun sequence.
ACCESSION   DBNCWK010000192 DBNCWK010000000
VERSION     DBNCWK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.193"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            57..854
                     /locus_tag="AC06IO_002749"
     CDS             57..854
                     /locus_tag="AC06IO_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9875910.1"
                     /translation="MPDNWVEIKNLFFSLDDRCIFDGIDMVVKQGKITAIMGPSGSGK
                     TTLLRLIGAQLRPDSGQIYVNGQNIHQLSRKSLYETRRNMGLLFQSSALFTHLSVFEN
                     VAFPLREHTELNASMIRNIVLMKLEAVGLRGAAHLMPAQLSGGMCRRVALARTIALDP
                     VLMMYDEPFTGQDPISMGVLVRLIQRLNQLLHTTTIIVSHDVEETCSIADYIYLISGG
                     KIIGQGTPDELKRSIEPQIRQFMHGEADGVVPFHYPAKPYTEELLNA"
     gene            847..1629
                     /gene="mlaE"
                     /locus_tag="AC06IO_002750"
     CDS             847..1629
                     /gene="mlaE"
                     /locus_tag="AC06IO_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HIO9875911.1"
                     /translation="MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLR
                     YQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKFGASSQLGQLLALSVARELGPVI
                     SALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
                     FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVY
                     QGFECVPTAEGISHATTKTVVYSSLAVLGLDFLLTAMMIGDW"
     gene            1630..2106
                     /gene="mlaD"
                     /locus_tag="AC06IO_002751"
     CDS             1630..2106
                     /gene="mlaD"
                     /locus_tag="AC06IO_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIO9875912.1"
                     /translation="MKKQRYVDISVGIFMLLGLLALLVMAMKVSNITDFMSDKTYRVT
                     ADFTDIGGLKVRAPVTVAGVRIGEVSRIELQPGELNARVTMTLNGDKKIPYEDASARI
                     LTEGLLGSNYISIVPGFDEDGDTDHPYLRDGDTIAKTQEAIILENLIGQLLFNIKK"
     gene            2114..2722
                     /locus_tag="AC06IO_002752"
     CDS             2114..2722
                     /locus_tag="AC06IO_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIO9875913.1"
                     /translation="MRVFKTILLVVLMTLSQAMVAQTSPIPMLERAANQIISILKENK
                     ARLKSNPEIIYKAVEENLLPNVDVAGMSRSVLGRQAWSKATEAERVEFSKAFTRLVIR
                     TYSSPLAQYSDETVQFLPLRGSLNSRFIRVNSVIVRSAGQNIPLSYSLVSKNGKWKIY
                     DISVEGVSLLQSFRSQFAQALQHSSISQVIKEMQQKQIKKAS"
     gene            2725..3006
                     /locus_tag="AC06IO_002753"
     CDS             2725..3006
                     /locus_tag="AC06IO_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIO9875914.1"
                     /translation="MEINNFKPGAELTFKSVVSVRDKLYKALMEDTRERFCLDLSEVT
                     HCDSAGLALIIEARKLCKQNNKVFDVIGISPETQSLAEFCGVKTILQRS"
BASE COUNT          952 a          523 c          747 g         1095 t
ORIGIN      
        1 tgatagatat caaactgaca tcattacccg ttaaaataat aagtggataa taaagcatgc
       61 ctgataattg ggttgaaata aaaaatctat tctttagcct tgatgatcgc tgcatttttg
      121 atggcattga tatggtagtt aaacaaggca agattaccgc tattatggga cccagtggtt
      181 ctggaaagac tactttactg cggttaattg gtgcgcaatt gaggcctgat agtggccaaa
      241 tttatgttaa tggtcagaat attcatcaat tatcccgcaa atcactttat gaaaccaggc
      301 gaaacatggg attgcttttt caaagtagtg ccttatttac acacctttca gtatttgaaa
      361 atgttgcctt tccattaaga gaacatactg aattaaacgc atccatgata agaaacattg
      421 tattaatgaa gctcgaagcg gttggtttgc gtggagccgc ccatctaatg cccgctcaat
      481 tatcaggggg aatgtgcagg agggttgcat tagccagaac cattgctctt gatcctgtat
      541 taatgatgta tgatgagcct tttaccggtc aagatccaat ttccatgggg gtattggtca
      601 gattaataca acgattgaat caattattgc ataccaccac tattatcgta tctcatgatg
      661 ttgaagaaac ttgttcaatt gcagattata tctacttaat atcagggggt aaaattatag
      721 gtcaaggtac tccggatgaa ttaaagcgat ctattgagcc tcagataaga cagtttatgc
      781 atggagaagc ggatggggta gtgcctttcc attatccggc aaaaccgtat acagaggagt
      841 tattaaatgc ttgatacaat cgcgcgtctg ggtaatcgag gtgtacaagc cttacaaggt
      901 attggtagtt cagggctttt tttattttcc atgttgttca gaaagcctaa tgttttaagg
      961 ttatggccat tattacgtta tcagctttat tttatcggtg tattgtcttg tttaattatt
     1021 gtggtttcag ccttgtttat tggtatggtt gtaggacttc aggggtataa tacgttacaa
     1081 aaatttggtg cttcaagtca attaggtcaa ttattggctt taagcgttgc cagagaatta
     1141 ggacctgtga ttagcgctct gttgtttgca gggcgtgccg ggtcggccct tacagctgaa
     1201 attggtttaa tgaaggcaac agagcagtta tccagtatgg atatgatggg agtggatcca
     1261 ttaggaagag tggtttatcc gcgttttgtg gcaggattta tagcgttacc gatacttgct
     1321 ttgatttttt ctgcagtagc tatatatggt ggttatttta ttggtgttta ctggctaggc
     1381 gtggatgcag ggagtttttg gtctaatatg caagctgctg taaattttcg tatagatatc
     1441 ttaagcggga ttattaaaag cttggttttc gcatttgtgg ttacttggat ttctgtatac
     1501 caaggttttg agtgtgtgcc aacagctgaa ggcattagtc atgctacaac taaaactgta
     1561 gtatactcat cgcttgctgt attaggtctt gatttcttgt tgactgcaat gatgattgga
     1621 gattggtaaa tgaaaaagca acgttatgta gacattagtg taggaatatt tatgttgctt
     1681 gggctgctag cattattagt aatggcgatg aaagtgagta acattactga ttttatgtct
     1741 gataaaactt atcgtgtcac tgcagatttt actgatatcg gtggtttaaa agttcgagct
     1801 ccagtgaccg ttgcaggtgt ccgtattggt gaggtcagtc gaattgagtt gcagccaggt
     1861 gagctcaatg caagagtgac tatgactttg aatggtgata aaaagattcc ttatgaggat
     1921 gcttctgcac gaattttgac cgaaggcttg cttggttcga attatattag tatcgttccc
     1981 ggttttgacg aggacggtga cacagatcat ccctatttac gcgatgggga taccattgcg
     2041 aagactcagg aagcaattat ccttgaaaat cttataggcc aattattatt taatataaaa
     2101 aaataggtga accatgagag tttttaaaac aattttatta gttgtgttga tgacgttgtc
     2161 tcaagcaatg gttgctcaga cctctcctat ccctatgtta gaacgtgcag ctaatcaaat
     2221 tatttctatt ctcaaagaaa ataaagcgcg cttaaaaagt aatcctgaga ttatttacaa
     2281 agcagtcgag gagaatcttt tacccaacgt tgacgttgct ggtatgtctc gttcagtctt
     2341 gggaagacaa gcctggagta aagctactga agcggaaagg gttgaattct caaaagcatt
     2401 tactcgcttg gtgattcgta catattctag cccattggcc caatattccg atgaaactgt
     2461 gcagttttta cctttacgcg gctctttaaa tagtcggttt attcgtgtaa atagcgtgat
     2521 tgtgcgttct gcagggcaaa atataccatt aagttatagt cttgtgtcca agaatgggaa
     2581 atggaaaatt tatgatataa gcgttgaagg tgttagtttg ttgcaaagtt ttagatcgca
     2641 atttgcacag gcactgcaac attccagcat tagtcaagtg attaaggaaa tgcaacaaaa
     2701 acagattaag aaggcttcct gagcatggaa attaataact ttaaacctgg cgctgagctt
     2761 acattcaaat cagtggtatc ggtccgtgac aagctgtata aggctttaat ggaagatacc
     2821 agagagagat tttgccttga tttaagcgag gtgactcatt gcgatagtgc tggacttgct
     2881 ttaattattg aagctagaaa attatgtaaa caaaataata aggtttttga tgtaatagga
     2941 atatctcctg aaacacaatc tctggctgaa ttctgtggag tgaagactat tttacaaaga
     3001 tcttgaaaca attctgagtc ataaatattt attattccat agagcttatc cctttagagc
     3061 gctagaatct atcgacattt ctactgtctt ggctttaaag cgttggtttt ctgtgttccg
     3121 ttgcttaata atggtttgat atttctgcta cggtgcttaa aaatcaaagt tttgggtttc
     3181 aagccagaga aaaattaaca gactctagtt gtcctcctga attgataaat tcccatgaat
     3241 tttgaggtaa tgttgtggtt ggtgaaacag tctaaatctt tagagaatga tggtgtaaac
     3301 acggattttt ttgataa
//
LOCUS       DBNCWK010000193         3292 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.194, whole genome shotgun sequence.
ACCESSION   DBNCWK010000193 DBNCWK010000000
VERSION     DBNCWK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.194"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1014
                     /locus_tag="AC06IO_002754"
     CDS             <1..1014
                     /locus_tag="AC06IO_002754"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9875915.1"
                     /translation="ERAFSLLFIQFSIMQIQNTEINIGVIIVNYNSAHCLAKAITALE
                     KQSYKPYQIILFDNSSIENLPEIVHTTKLPIKIVVSNINLGFAAGNNKAIEYLDPKVN
                     WIALLNPDAYPHKDWLLEMVSAIKTHPQYAFMGSKLICENEPYLLDGTGDVYHISGKA
                     WRLNHRKPLDKAPKDMQELFAPCAAAALYRRDAFNAVKGFDEKYFCYYEDVDLAFRLW
                     LAGYKGCYIPTAIAEHTGSAITKKHSDFYTYYGHRNLVWTYFKNMPLLLLIATLPLHL
                     LLNIATLLLFIKRKQTFVIIKSKIDAIKGLPALIQQRRHIQKSRILSNFQLLKILKKG
                     LPW"
     gene            1008..1901
                     /locus_tag="AC06IO_002755"
     CDS             1008..1901
                     /locus_tag="AC06IO_002755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIO9875916.1"
                     /translation="MVANFVTVVIVNYNSGEMLSKCVKHLLKSTVQVQIIISDNASSD
                     NSLALLEQLELNKQTITIHRNNSNYGFSYANNAVFTKISTPFILYLNPDCFVEKDTIE
                     NLLHTMHQHNDAGMVGCLVNNMDGSVQTGCCGLTPTPARVLNQLLKLSKIFPNNPKFG
                     GYLFSKEKLPTKPTEVELISGSCMFVRKKAIDEVGLLDDNYFLYCEDYDWFYRFTQSN
                     WKIIFNPNTKVTHIKSFSTKQIPFKVLLYKAKGMWRFYNKFFKQNSSPATTIIIRVGI
                     IARFFSLSGLLLFKKLLRTTN"
     gene            complement(2070..3143)
                     /locus_tag="AC06IO_002756"
     CDS             complement(2070..3143)
                     /locus_tag="AC06IO_002756"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIO9875917.1"
                     /translation="MYNKLTTSQATYLNFLRGFSAIIVLAGHTLSGIPGVISFGKQLP
                     FQSLAVNAFFWLSGFLITYHCITKQPYSFVEYMIDRFCRIYVIYIPVLILSVLLLVWV
                     DLVSMPEIKDWVANIFMIQHTPFNRIFEFLPTIPPLAQISPLWSIAVEWWLYTLFGIA
                     FFFHKSSFATRFIMSLLIIPALLVAGYFTLKEYVALVWFLGSGCAYHFCNINKKYNNN
                     AILMLSLITGTAFLVRFYILKHSIMNMYDLQLVIPGCIFLYSLLLLLSANKLNKKIEL
                     ISAFLAFISYTLYLSHEPIRRVVSTFIEPSNLKRGFFICGVCIACATLIAYLLENKHL
                     LVREWMKNKFLQKNTSNKLAYIT"
BASE COUNT         1161 a          571 c          526 g         1034 t
ORIGIN      
        1 gaacgcgctt tttctttgct cttcatccaa ttttcaatta tgcaaattca aaatactgaa
       61 attaatatag gcgttataat tgttaattat aattcagcac attgcctcgc taaggcaatt
      121 actgctttag agaagcaatc atacaaaccg tatcaaataa tactgtttga taattcgagc
      181 attgaaaatt tacccgagat cgtccacaca actaaattac caattaaaat tgttgtatcc
      241 aatattaacc tgggatttgc agctgggaac aataaggcca ttgaatatct agatccaaaa
      301 gtcaattgga tagcattgct taatcctgac gcctacccgc acaaggactg gttactagaa
      361 atggttagtg caattaaaac tcacccgcaa tatgctttca tggggtcaaa actaatttgt
      421 gaaaatgagc cttatctatt agatgggaca ggcgatgtgt accatattag cggaaaagca
      481 tggagattaa accacagaaa accattagac aaagccccca aagatatgca agaactgttt
      541 gccccctgcg ctgcagctgc cttgtataga cgtgatgcat tcaatgctgt aaaagggttc
      601 gatgagaaat atttttgtta ttatgaagat gttgatttgg cttttcgatt atggctagca
      661 ggttataaag gatgttatat tcctacagct atcgctgagc atacaggctc cgccattaca
      721 aagaaacata gtgattttta tacttattat ggccatagaa atctagtttg gacctatttc
      781 aagaatatgc cattgttatt gcttattgca actttaccct tacacctcct tttaaatatt
      841 gctactcttc ttttattcat taaacgaaag caaacttttg taattataaa atctaaaatt
      901 gatgcgatta aaggtttgcc agcactaata caacaaagaa ggcacattca aaaatcgaga
      961 attctttcta actttcaatt actaaaaatc ttgaaaaaag gcttgccatg gtagctaatt
     1021 tcgtaacagt tgttatagtt aattataatt cgggcgaaat gcttagtaag tgcgtaaagc
     1081 atctattaaa aagtacagtt caagttcaaa ttattatttc agataatgcc tcctctgata
     1141 atagtttagc gttattagaa caacttgaat taaataaaca aactattact attcacagaa
     1201 ataattccaa ctatggattt tcttacgcca ataatgcagt ttttacaaaa atctcaaccc
     1261 catttatact ctatcttaac cctgattgtt tcgttgaaaa ggataccatt gaaaatcttt
     1321 tgcatacaat gcaccaacac aatgatgctg gaatggttgg atgtctggta aataacatgg
     1381 atggtagcgt gcaaactggc tgttgtggac ttactccaac cccagccagg gttttaaatc
     1441 aactattgaa actatcaaaa atcttcccga ataaccctaa atttgggggg tacttatttt
     1501 caaaagaaaa gctcccgaca aaaccaactg aagtagagtt gatttcaggg tcttgtatgt
     1561 ttgtacgcaa aaaagcaatc gatgaagttg gccttctgga tgataactat ttcctatatt
     1621 gtgaggacta cgattggttt tatcgattta cacaaagtaa ttggaaaatt atttttaatc
     1681 cgaatactaa agttactcat ataaaaagtt tttccaccaa acaaattcca tttaaagtac
     1741 tgttgtataa agcaaagggc atgtggagat tttataataa attctttaaa caaaattctt
     1801 ctccagctac cactattatt attagagttg gaattatcgc gagatttttt tctttaagtg
     1861 gtttgcttct ttttaagaag ctgttgcgta ctaccaacta gaaaattcac cgcctctaac
     1921 tccacgtaac cgtacacgca atggagtcaa cttaaggtga tgtcttattt agcaaacccg
     1981 ccaaaaggac tgatgccgct tgcggttttc ccattttatc ctaataatta acaggtttac
     2041 ccaaaagaca aataaatcgg ataatttgct tacgtaatat aagctaactt atttgatgtg
     2101 tttttttgca aaaatttatt tttcatccac tcacgaacca gtagatgttt attctctagt
     2161 aaataagcga tgagagttgc acaagcaatg caaactccac agataaaaaa acctctcttt
     2221 aaattagatg gttcaataaa tgttgatact actcttctta ttggctcatg gctcaaatat
     2281 agtgtatagg aaataaacgc aaggaatgct gatattaatt ctattttttt gtttagtttg
     2341 ttagctgaca gtaataacag cagtgaatat aagaagatac aaccagggat tactaactgt
     2401 aaatcataca tattcattat tgaatgcttc aatatataga atctaactag aaaagctgtt
     2461 cctgtgatta ggcttagcat taaaattgcg ttattattat acttcttatt tatattacaa
     2521 aaatgatatg cgcacccaga acccagaaac catactaaag caacatactc ttttaatgtg
     2581 aagtatccag caactagtaa tgcaggaata atcaataaac tcattataaa cctggtagca
     2641 aaagagcttt tatgaaaaaa aaaggctatt ccaaacaaag tatataacca ccactcaact
     2701 gctattgacc acaatgggga aatttgagct aaaggtggaa tagtaggtaa aaattcaaaa
     2761 attcgattaa aaggtgtatg ttgtatcatg aaaatattgg caacccaatc tttaatttcg
     2821 ggcattgaca ccaaatccac ccatactaac aacaaaacag ataaaatcaa tactggaata
     2881 taaataacat aaattctgca gaaccgatca atcatgtatt ctacaaatga atagggttgc
     2941 tttgttatgc aatgatatgt aatcaaaaat cctgagagcc aaaaaaaagc attcactgcc
     3001 aaggactgaa aaggcagttg tttcccaaat gaaattaccc cagggatacc tgataaagta
     3061 tgtccggcca agactattat agctgaaaac cctcggagaa aatttaggta ggttgcttgg
     3121 cttgttgtga gtttattata catttaaaaa accattcctt tagatagtat caacaataaa
     3181 ttaaattgtt ttcaaattat atataaaaaa aataagtaaa gaaattaaat tagttaaatt
     3241 attcttgcat gtaatttagg taatatcact ttgattcttg caagccttgc aa
//
LOCUS       DBNCWK010000194         3230 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.195, whole genome shotgun sequence.
ACCESSION   DBNCWK010000194 DBNCWK010000000
VERSION     DBNCWK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.195"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(14..1810)
                     /locus_tag="AC06IO_002757"
     CDS             complement(14..1810)
                     /locus_tag="AC06IO_002757"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIO9875918.1"
                     /translation="MPKTPNNCSLSSGNKSPTFSRILFSYMTEYRWSTAFFIFFGLFW
                     AFTLPYMSYLLGQIIDEIKFQNPEQVDIFSLVLTSLTLYVAIHVLRSLGYYVHGLFSL
                     TSIPEKKAKLIKQLFNHLGNQSIGYFEDKHSGYLTNKITNASSSIELLIFNLFTIIYP
                     QSLAIILTGILLSLVVPYFGVVLWLWGAALIIYSYHSAKIGNDKAMLLANSNSKVNGK
                     VVDVVNNIHTVIYNSEMSFELGNLDTDIQDMVQNDRNLQRHMTKVMLVQHIAMNILVA
                     FFLIGSVLGYNYGYVSVGDIVFVMTSVTAIAGLTSSLGKCFLDFVTNAGRLNEGLSLL
                     GDQHTITDNANAQAHQINNGEIQFHDISFAYPNQPPIFLNFNLTIPAKQKIGIVGSSG
                     AGKSTLFKLLMRLYNVEKGVIAIDNLPINAYTKKSLCSQIAIVPQHLTLFHRSIYDNI
                     TYGCNNFDKSDVILAAKKAHCHEFIAELPEQYDTIIGEQGVKLSGGQRQRIAFARAIL
                     KNAPILLLDEATSALDSKTEQAIQDGLQNLLADKTALVIAHRLSTLKAMDRILVLENG
                     KIIENGTHTELLEKRGAYYSYWSHQSEGFIRA"
     gene            complement(1844..2710)
                     /locus_tag="AC06IO_002758"
     CDS             complement(1844..2710)
                     /locus_tag="AC06IO_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HIO9875919.1"
                     /translation="MKNRRDEKTMLTLIIKIANDDERIKIVIMNGSRASPSAKKDIFQ
                     DYDIVYLVTEIESFVHDKNWINQFGELLIMQTPDEMDGQWPKFKGKYTYLMQFKDWNR
                     IDLTLLRMDQLETMPRDSQSLLLLDKDNLIAPFGEPSDEDYLPSPPTEKDFVNCCNEF
                     LWVSTYVAKGLWRKQLIYAKHISEQIVKEELIKMLMWHAGIQTNFSQPMGIYGKYIEN
                     HLELELRQEFLKTYVDADYKNMWASLFKMCEIFNDLAIKISSYFGYSFNQKEFDNVID
                     YLQDVKNNKIPP"
     gene            complement(2756..>3230)
                     /locus_tag="AC06IO_002759"
     CDS             complement(2756..>3230)
                     /locus_tag="AC06IO_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875920.1"
                     /translation="NPIVAEYITGTSSGVMLVDSYEKLLELSKINPAVADNMTRTQLG
                     LDLIDSYEKFLEITKINAKVAFNFRILNYQRYTKLVDSYQQSSQEKSLSDLNTSANSQ
                     LFFKKSDSTRQDLASISTGQARLAHDTVGLELEWNEAIRNTISENMMNRNFNSKP"
BASE COUNT          981 a          578 c          583 g         1088 t
ORIGIN      
        1 gtagcagatt gtttcatgct cgtatgaaac cttccgattg gtgtgaccag tagctataat
       61 atgctcctct tttttccaat aattcggtat gtgttccatt ctcgatgatt ttgccatttt
      121 ctaaaactag aattctatcc attgctttga gcgttgatag acggtgcgcg atcactaaag
      181 cagttttatc tgctaacaaa ttttgtaagc catcttgaat tgcctgttca gtcttggagt
      241 ctaaggctga cgtcgcttca tcaagtaaca agataggtgc atttttgaga atagctcggg
      301 caaatgcaat acgctgtctt tgtcctcctg ataattttac accttgctcg ccaataatgg
      361 tgtcatactg ctcaggcagt tcagcgataa actcatggca gtgtgctttc ttcgcagcta
      421 aaatcacatc tgatttatca aaattattgc aaccgtaggt gatattatca taaatgctac
      481 gatgaaaaag tgttaaatgt tgtggcacta ttgcaatctg agagcaaagt gattttttag
      541 tgtaggcatt tattggtagg ttatctattg caatgacacc tttttctaca ttgtataatc
      601 gcatcaataa tttgaataat gtgcttttac cagcccctga actacctaca attcctattt
      661 tctgctttgc tggaatcgtt aaattaaaat ttagaaaaat tggcggttga ttgggataag
      721 caaatgaaat gtcatgaaat tgaatctcac cgttattaat ttgatgtgct tgagcatttg
      781 cgttatcagt aatcgtatgt tgatctccca ataagctcaa cccttcattt aaacgcccgg
      841 cattagtaac aaaatcgaga aagcatttcc caaggctgct tgtgaggcct gcaatagcag
      901 taactgatgt cataacaaaa acaatgtcgc ctacactgac ataaccataa ttatacccaa
      961 gcactgagcc tattaaaaag aaagcgacga gaatattcat tgcaatgtgc tgtaccagca
     1021 tgactttggt catgtggcgc tgcaaattgc gatcattttg aaccatatcc tgaatgtctg
     1081 tatccagatt accaagttca aaagacattt cagaattgta gattactgtg tggatgttat
     1141 tgaccacatc gactacctta ccgttgactt tactattgga attcgcaagg agcattgctt
     1201 tatcattacc aattttggcg gaatggtatg aataaatgat aagtgcagct ccccagagcc
     1261 agagaaccac tccaaaatac ggtacgacta aagacaataa tatgcccgtc aggataattg
     1321 ctaacgattg tgggtaaata atggtaaata aattaaaaat aagtagctca atactagaac
     1381 tggcattggt aattttgttt gttaaataac cggagtgctt atcttcaaaa taaccaatgg
     1441 attgattgcc caagtgatta aatagttgtt taatcagttt ggcttttttt tcgggaatac
     1501 tggtaagtga aaacaaacca tgaacgtaat atcccaagct tcgcaaaaca tgaattgcaa
     1561 cataaagagt taatgaggtt aggactaagc taaagatgtc aacctgttca ggattttgaa
     1621 attttatttc atctatgatt tgcccaagta agtaggacat ataaggtaga gtaaatgccc
     1681 aaaaaaggcc aaaaaaaata aaaaatgctg tactccagcg gtattcagtc atatatgaga
     1741 acaagattct tgagaaggtt ggtgatttat taccgctgga taaactacaa ttgtttggag
     1801 ttttgggcat aatatttcct taattccatg tggatttgtt cgattaaggt ggaattttat
     1861 tgttttttac atcttgcaaa taatctatca cattatcgaa ttctttctga ttaaatgagt
     1921 aaccaaaata acttgaaatt tttatcgcca ggtcattgaa tatttcacac attttgaata
     1981 gtgaagccca catgttctta taatcagcat caacgtaggt tttgagaaat tcttgccgga
     2041 gctcgagttc aaggtggttt tcaatatatt ttccataaat tcccatcggt tgactaaaat
     2101 tggtttgaat tcctgcatgc cacatgagca ttttaattaa ctcttcttta actatctgct
     2161 ctgaaatgtg ttttgcgtag atgagttgtt ttctccagag tcctttggca acgtaagtgc
     2221 ttacccataa gaattcatta caacagttaa caaaatcttt ttctgtaggg gggcttggta
     2281 agtaatcttc atcactaggt tcgccaaaag gtgcaattaa attgtcttta tcaagcaata
     2341 agagactttg actgtctcta ggcatagttt ccagttgatc catgcgtaac agggttaaat
     2401 caatcctgtt ccaatcttta aattgcatca gataagtata tttaccttta aattttggcc
     2461 attgcccatc catttcatca ggtgtttgca tgataagtaa ttcaccaaac tgattaatcc
     2521 aatttttatc atgaacaaat gattctattt cagttaccag atacacaata tcataatcct
     2581 gaaaaatatc ctttttcgct gaagggcttg ctcgagagcc attcataatc acaattttaa
     2641 tcctctcatc gtcattagct attttgatga tgagtgtaag cattgttttt tcatcacgtc
     2701 gatttttcat gtttctggat tatggccttt ttaaatttaa attctagaat agctatcagg
     2761 gtttgctgtt aaagtttcgg ttcatcatat tctcgctaat ggtattccgg atagcttcgt
     2821 tccattccag ctccaatcca actgtatcat gagctaatct cgcttgtcct gtgctaatag
     2881 aagctaggtc ttgtctagta gaatcgcttt tcttaaaaaa aagttgggaa tttgccgagg
     2941 tatttaggtc tgaaagagat ttttcttgtg aagattgttg atatgagtcg accagcttgg
     3001 tatacctttg ataatttaat attcgaaaat taaaagcaac tttagcattt atctttgtta
     3061 tttccagaaa tttttcatag ctatcaatta aatccagacc caattgagta cgtgtcatat
     3121 tatcagcaac agcagggtta attttagaga gctctaatag tttttcatag gaatcgacca
     3181 gcataacccc tgatgaagtc cctgtaatat actctgcaac aatgggatta
//
LOCUS       DBNCWK010000195         3200 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.196, whole genome shotgun sequence.
ACCESSION   DBNCWK010000195 DBNCWK010000000
VERSION     DBNCWK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.196"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            293..739
                     /locus_tag="AC06IO_002760"
     CDS             293..739
                     /locus_tag="AC06IO_002760"
                     /inference="COORDINATES: protein motif:HMM:NF046565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875921.1"
                     /translation="MLDPLTLEEIQESCDILKREKELSDSYRFAWVMTYEPPQQEVLH
                     SSDTDFDRCAFLSVFNKKTNETFEAIVNLNSKKVVEWKQIVLEASPYGKPLILIEDFQ
                     KCEQIVKADPTWRAAMEKRGLNDEQIDKIQVDAFSAGYFDEEEEQA"
     gene            complement(875..1465)
                     /locus_tag="AC06IO_002761"
                     /pseudo
     CDS             complement(875..1465)
                     /locus_tag="AC06IO_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_099156038.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1676..2662)
                     /locus_tag="AC06IO_002762"
     CDS             complement(1676..2662)
                     /locus_tag="AC06IO_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HIO9875922.1"
                     /translation="MFCIKKTALAVLALGSSAAFAGTMGPACTPGNVTVPCTTTGWDV
                     GIYALYLEPSYNGHPRFFNYVGSDFVGAQRVHTKLNTDFDWGFKLEASYHFSTGNDIN
                     VNWYHWDTTTSQNLHYFGEISGAEVFFTPFQIDHKWDAVNFEFGQHVDFGEFKNIRFH
                     GGAQYARLQHKIYSPYTINIPSAGFVVDPDTGNIVLSPAETEIGANRIDLTFRGVGPR
                     VGADMSYDWPNGFAIYANGAAALLIGDNKVNDTSDIEPNRATHTSIVPELEAKLGLTY
                     TYAMGQGNLTLDGGYMVANYWNAFHVVQAGQDGSTDFGVHGPYLGLKYIGAL"
BASE COUNT          982 a          633 c          608 g          977 t
ORIGIN      
        1 attcaaattg attagaatga tataaagtgc acccttaatg gttgcagccc ctagcgcgag
       61 caccagaagg ttaaataatg taccactttt aaaaggccag acttttaatt tgaaaagctt
      121 atgttgtatt cactgattaa aaaatactat actgcccacc gcttatccaa ctattcttaa
      181 cgatatcccg ctatttttat ttctatctaa ttacttcaaa gcactgtctc tttagttaac
      241 tttttaaaga gcaatattag ttttttaatg agtaaaggat gtcaatatac atgtgttaga
      301 tccattaact cttgaggaaa ttcaagaaag ttgtgatatt ttaaaacgag aaaaagaact
      361 gtccgacagt tatcgatttg cctgggtgat gacttacgaa ccgcctcaac aagaagtttt
      421 acattcaagc gatactgatt tcgaccgctg tgcttttctc tctgttttta ataaaaaaac
      481 gaatgaaacc tttgaagcaa tcgtaaacct caacagtaaa aaagtcgtgg aatggaaaca
      541 gattgtcctc gaagcctcgc cttatggtaa gccgcttatt ttaattgagg attttcaaaa
      601 atgtgagcaa attgttaaag ccgatccgac ctggagagcc gctatggaaa agcgtgggct
      661 taatgatgag caaattgaca agattcaagt cgatgcgttt tctgcgggct atttcgatga
      721 agaggaagag caagcgtagg tcgggccttg gcctgacaat tccttgatta tattcaaaaa
      781 ggaattataa ccagcgcttg ggctttctcg gatacattac acacgacaaa tcagtttgaa
      841 gattaacatt gattttgtcg gaccaaggcc cgacctacgc ttgccgaccc tttttgaaca
      901 tgcgcatgat tttaaacccc ttgatggtcg cataggctgt tttcattgat tgaaagccac
      961 acacgggatt aataagtggc ttcagttttc catgatctgc ttccagccga ttattacggt
     1021 attttatttg caaatgacgg atataagaag ctaaattacc ttcttgcttc agctctttaa
     1081 tagtctgacc ataagctggg tttttatctg tgttgatgac actgatatcg caggctgggg
     1141 ttattcttaa taagtttttt aagaaaccgt ttagccgcaa tagcattacg tcgatgagac
     1201 aaataaaaat caatggtatt tccttgctca tcaatggcac gataaagata tttccattct
     1261 ccctttatct tgatgtaggt ttcgtccaag tgccagcgtc aagagtaaca tttcttatgc
     1321 cattcgagtc attttttgag ttctggagca taatgctgaa tctagtagta tgatctaact
     1381 caagacctcg ttcctcgatc atttctttca aatctctata gctgatgccg tatttacaat
     1441 accaacgcac acattgcaga attatctcac cgtgaaagtg acgtcattta aatgcaggag
     1501 gttttgttga acgcatctca tatttccatt agtttgatct ggtgagtgta agttataaac
     1561 taatatcttt tttgcaacgg agccttaaaa cttacggtaa tgtaaaccca taatcaacag
     1621 ataatctata aggaagtggg aaacctgata gatatctgct gatcacggca tcaaattaca
     1681 acgcgcctat atatttcaga ccaaggtatg gaccatggac gccaaaatca gttgaaccat
     1741 cctggccagc ctgaacaaca tggaaggcat tccaataatt agcaaccata taaccgccat
     1801 caagtgttaa attaccttgt cccattgcat aagtataggt aagacctaat tttgcttcca
     1861 gttcaggaac aattgaagta tgagtagcac ggttaggttc aatatctgaa gtatcgttga
     1921 ccttattatc accaattaaa agggctgctg caccatttgc atagattgcg aagccgtttg
     1981 gccaatcata ggacatatca gcaccaacac gtggaccaac tcctctaaac gtcagatcga
     2041 ttctattagc acctatttca gtttcagctg gtgaaagaac tatatttccg gtatcaggat
     2101 caacaacgaa ccctgctgat gggatgttta tagtataagg tgagtatatt ttatgttgca
     2161 aacgagcata ttgtgcacca ccatggaacc gaatattttt aaattcacca aagtctacat
     2221 gttgaccgaa ctcaaaattg acagcatccc atttgtgatc aatttggaaa ggagtaaaaa
     2281 atacttctgc accacttatc tcaccaaagt aatgtaaatt ttggctggta gtagtatccc
     2341 agtgatacca attgacatta atatcattac cagtactaaa gtgataggaa gcttctaact
     2401 tgaatcccca gtcgaagtca gtatttaatt ttgtgtgtac tctttgagca ccaacgaaat
     2461 cactaccaac ataattgaag aatcttggat ggccgttata agaaggttct aaatataagg
     2521 catagatacc tacatcccaa ccagttgttg tacaaggaac agttacatta cctggtgtac
     2581 acgcagggcc cattgtacct gcaaatgctg cactgcttcc taaagctagt actgctaaag
     2641 cagttttctt tatacagaac atactttgat ctccactatt attataattt ttcattattg
     2701 ataaaacagt ctccgaatcg tcgataatat gcattcgtta ccccgctgcg cgcatgaaag
     2761 gcggaacgcc gaggtgcgta gtccgagata agctgcaatt tacgcagaca gcccgaaggg
     2821 atgcggagta aatctttatg cagccgtaga ctcattggtg caggcatacg ctttgttcgt
     2881 tcagagcaaa cgtgcgggaa gcaggacggt tgcggtcttt aaactgtcgc gttggtgaat
     2941 gaattctggg gacttggatg tcccagaatt tataacgaag tagcagcacg ctttaacgaa
     3001 tcatatggct ataatattca cataaatgca ataatttcaa attttttcaa gaaaaattat
     3061 tatttataat gatgttatta attttttcac agcagaagca tttactggac atactaattt
     3121 tttggtgaat gcaacctcta ttcccttctt cgatcaaagt ggctttaatt ataaactatc
     3181 actcgttttt gcaacggagc
//
LOCUS       DBNCWK010000196         3184 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.197, whole genome shotgun sequence.
ACCESSION   DBNCWK010000196 DBNCWK010000000
VERSION     DBNCWK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.197"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(285..1676)
                     /gene="fumC"
                     /locus_tag="AC06IO_002763"
     CDS             complement(285..1676)
                     /gene="fumC"
                     /locus_tag="AC06IO_002763"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIO9875923.1"
                     /translation="MAKTRVETDSMGEIEVPSDKYWGAQTERSLHHFNIGKDIMPREV
                     THAFGILKKAAALTNLELGKLPKDKADLIVQAAEEVSKGLLDEHFPLHVWQTGSGTQS
                     NMNANEVISNRAIELADGTLGSKSPIHPNDHVNMSQSSNDTFPTAMHIAAAIAFNEKL
                     LPAVRNLRHVFAAKMDAFKNIIKIGRTHLQDAVPLTLGQEFSGYVAQLDACIHRLEEV
                     LPELYELAAGGTAVGTGLNTHPQFAVKVAEHIAKITKLPFVTAPNKFAALASHEPLVH
                     AHGAMKTLACALMKIANDIRWLASGPRCGIGELIIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDTAVGIADSQGNFELNVFKPVIIFNVLHSLNLLADSCHSFQEFCAEGIE
                     PNQPVIDYYLHHSLMLVTALNQHIGYDKAAKIAKTAHHDNISLQEAAVKLGILTAEQF
                     AAFVKPEEMIAPK"
     gene            1839..2573
                     /locus_tag="AC06IO_002764"
     CDS             1839..2573
                     /locus_tag="AC06IO_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg2936 family Dot/Icm T4SS effector
                     methyltransferase"
                     /protein_id="HIO9875924.1"
                     /translation="MAVRTIRIYQPGEYQPGQLLELSPEAGQHVGVVLRMEPGEQLTL
                     FNGDNKEFTASIEMVKKKQVFVRIASVLEVNRESPLKIHLAQAISKGERMEMVMQKSA
                     ELGVACITPIITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQ
                     FVREAKEHLKLILHPAFSKTWRDYPVQHPDVALIIGPEGGFSDEEIRLTSGHGFLPLS
                     LGPRVLRTETAAITALSVLQAAGGDL"
     gene            2786..3112
                     /gene="trxA"
                     /locus_tag="AC06IO_002765"
     CDS             2786..3112
                     /gene="trxA"
                     /locus_tag="AC06IO_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIO9875925.1"
                     /translation="MSDHIKTVTDASFEQDVINSSKPVLVDFWAEWCGPCRALTPILE
                     EVAATHSKDMTFAKINIDENPQAPAKFGVMSIPTLILFKNGQVEAVKMGLLSKSQLSA
                     FVESNL"
BASE COUNT          885 a          615 c          724 g          960 t
ORIGIN      
        1 tatctgcatt agcataatta cctttacgca gaagcatatg ttttccatat cgtgtagtgc
       61 tgaatttgaa taaataatga gaaatagttt atctcacctg cttataattt tttgatctct
      121 taatctatca atctgattag tgaaacttca gtacccatat tttaattaaa acactcctgt
      181 ttttcattga gagttcaaag gcagcgttac aaataaggct atagaaattt ttccatagcc
      241 taaaaatcaa agagcgattc aatagagctg tacattacaa aaaactactt tggagcaatc
      301 atttcttccg gcttcacaaa cgcagcaaat tgctcggcgg ttaaaattcc cagtttcacg
      361 gcagcttctt gcaatgaaat attgtcatga tgagcggttt tggcgatttt agccgcttta
      421 tcatagccaa tgtgctggtt aagagcagta accagcatga gggagtggtg taaatagtaa
      481 tcaattaccg gctgattagg ctctattcct tctgcgcaaa attcctggaa ggaatggcag
      541 ctgtcggcaa gcaggtttaa tgagtgcaat acgttaaata tgatgaccgg tttaaacacg
      601 tttaattcaa aattgccctg gctgtcagca attccaacgg ctgtatcatt acccagtacc
      661 tgggcgcaaa ccatggtcat ggcttcgcat tgtgtcgggt tgactttgcc tggcatgatg
      721 gatgagcctg gctcattttc tggaataatc agctcaccaa tcccgcaacg aggtcctgat
      781 gcgagccagc gtatgtcatt ggcgattttc attaatgcgc aggcgagagt tttcatggca
      841 ccgtgcgcat gcaccagagg ttcgtgagaa gccagagcgg caaatttgtt aggggcagtc
      901 acaaaaggca gtttggtgat tttagctata tgctcggcga ctttgactgc gaactgcgga
      961 tgggtgttca atccggttcc aacggcagtg cctccggcag ccagctcata taattcaggc
     1021 aatacctctt ccagacggtg aatgcatgca tccagttggg caacataacc tgaaaactct
     1081 tgccccaaag ttagtggaac cgcatcttgc aagtgggttc tgccaatttt tatgatgttt
     1141 ttgaaagcgt ccatttttgc tgcgaaaaca tgacgtaaat tgcgtacagc aggtaacaat
     1201 ttttcgttaa aagctatggc cgctgcaata tgcatggctg ttgggaaggt gtcattcgat
     1261 gattgagaca tattgacgtg atcattggga tgaatagggg acttgcttcc cagggtgccg
     1321 tcagccagtt caatggcccg attggaaatg acttcattgg cgttcatatt cgattgtgtg
     1381 ccgcttcccg tttgccagac atgcaaggga aaatgctcat ccaacaggcc tttgcttact
     1441 tcttctgctg cctgaacaat caaatccgct ttgtcctttg gcagtttgcc taattcaaga
     1501 ttcgtcaggg cggccgcctt tttcagaata ccaaaggcat gggttacttc gcgtggcata
     1561 atgtctttgc ctatattgaa gtgatgcaat gaacgttcgg tttgggcgcc ccaatattta
     1621 tcagacggga cttcaatttc acccatgctg tctgtttcta cacgtgtttt agccatgtta
     1681 tcaattcctt agcaaagtta atctgttgat tatgtttatt tatgtctagc tgctttatca
     1741 gcgagttggg aaagaataaa atcaatcgcc aaaattctag catagcagac gtaaattggt
     1801 aatttttgtt atagtgtcat tatcgaaatg aggaataatt ggctgtgaga acaatacgta
     1861 tctatcaacc tggtgaatac cagccagggc aacttctgga attatcgcct gaggcggggc
     1921 agcatgttgg tgtagtcttg cgcatggagc caggtgaaca actgacactg tttaatgggg
     1981 acaacaaaga gtttacagca tccattgaga tggtcaaaaa aaagcaggta ttcgttagaa
     2041 ttgcctctgt tcttgaggtg aacagggaat ccccattaaa gatacatttg gctcaagcca
     2101 tttccaaggg cgagcggatg gaaatggtga tgcaaaaatc ggcagagtta ggcgtggcat
     2161 gcattactcc tatcattacc gagcgttgcc aggttaaaat agataaagaa aaaatggcaa
     2221 aaaaaatgca ccaatggctt aatattatca ttggagcttg cgagcaatgt gggcgtaatc
     2281 aaattccaga gttgcgacag ccagtttatt tggatcaatt tgtcagagaa gccaaagaac
     2341 atctcaagct cattttgcat ccggcattta gcaagacatg gcgcgattat cctgtccaac
     2401 atcctgatgt tgccttgatt attggcccgg aaggtggttt cagtgatgag gaaattagat
     2461 tgaccagtgg tcatggattt ctgcctttgt cactggggcc gcgagtattg cgtacggaaa
     2521 ccgcggcaat aactgcgtta agtgttttgc aagctgcagg aggtgatcta taatagactt
     2581 ctttccaaat cggtataagc tgcgtctttt gttttatagc tctattacaa gtatttctgg
     2641 agtattatat gtaaactact ctttttgaaa atcttgtatt tgctctaaaa caaaacacac
     2701 aggatacaca ggaaagattt tggatcgaat agcataatct ggtcttggac cagaccttta
     2761 tatttctaat aactgaggtt tatttatgag tgatcatatt aaaacagtaa ccgacgcaag
     2821 ctttgagcag gatgtaatta attcatccaa gcctgttctt gttgattttt gggctgagtg
     2881 gtgtggacca tgccgtgcat tgacacctat tctggaagaa gtggcagcca ctcatagtaa
     2941 agatatgacg tttgctaaaa tcaatatcga tgaaaatcct caagctccag ctaaatttgg
     3001 tgtgatgagt atcccaactt tgattctttt taaaaatggt caggttgaag cagttaaaat
     3061 gggtttgctg tctaaatctc aattaagtgc ttttgttgaa agtaatctgt aacggtatta
     3121 aagttaaaag gccctgagat gaaaatttat ttacagggct tttacttttt tatggggctt
     3181 gtgt
//
LOCUS       DBNCWK010000197         3123 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.198, whole genome shotgun sequence.
ACCESSION   DBNCWK010000197 DBNCWK010000000
VERSION     DBNCWK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.198"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            181..1857
                     /locus_tag="AC06IO_002766"
     CDS             181..1857
                     /locus_tag="AC06IO_002766"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIO9875926.1"
                     /translation="MLKKTMFFILSISTSSIFAADNVDLYQAPLNSINKYPILQTPKN
                     AIILKSSSAVIDNSLQKLNQTKEDNQMIVRYQQLYKGIPVIGAQVMITKGTDSGVQAN
                     DNAEVNGHLLDNIELNTKPAISAQQAKEYAKKSYFQFSPQSNIQQETAELQIRPDHNN
                     QLKLVYLVSFKSVQQDGKPDWPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYW
                     YGKDGLPALDVTQNGSQCVMENSKVKLVNLHSQWDWENTINTPFEYVCNNNIEENING
                     GFSPGNDAYYFGHVIVGMYKDWYGLNALQHSNGAPMQLVMRVHFGQNYDNAFWDGQAM
                     SFGDGLDFYPLVSLDVAGHEVTHGFTEQHSGLEYHDQSGALNESLSDMAGQASRAYLL
                     EKNPQLYNKAYLQPNEVTWGIGETIVRDSYGKALRFMDYPSSDGSSADCLDKGIAQNN
                     GSYCAISYDEVVAYANTHIALPQERQSFIVHTASGVFNKAFYLMSKDMGIKNAYHIMV
                     VANTKYWTPTTDFKNGACGVLYAARDLNTDINKVKSAFGQVGIDTAGCVI"
     gene            2078..2989
                     /locus_tag="AC06IO_002767"
     CDS             2078..2989
                     /locus_tag="AC06IO_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIO9875927.1"
                     /translation="MAFFLITLCILILSLFCLAVMVLKFIQQPSLPLSFSQYVKLMCS
                     GVIAFIADTVGVGSFAVNVALAKLLGTFRDDELPAVNNGAQVLPGAIESLFFIHMVDV
                     DLTTLLTLVTGTCIGGLVGGFVVSQLSKQAIRLAMICSFALIIVLLIAHQLRLLPVGG
                     DLTELHSWKLVIGFFAMVVCGSLTSVGVGLFVMVQGVLFLMNVSPVVAFPIMTTAGAM
                     QQPLTTLVFLQKNKIPLKKTLILSFSGCLGVMITIPIFTKLTITWLHFLLLFILIYNF
                     LAIGHTYLRSRPVKQYVQSSVKLAAAD"
BASE COUNT          922 a          525 c          623 g         1053 t
ORIGIN      
        1 ttcacaaaat ttcctcccgt aaatttgtaa aacacacaaa ggatgaattt ttcctaaaat
       61 cagttatcac gttaatcaga aaaattgata tgaatatgac atgaatatga aaaaaaatat
      121 tgcataatgt ttaattatgt gacattattt gcgccattgt gtattctgtg agagttgtgt
      181 atgttaaaaa aaacaatgtt ttttattttg tcgatatcta cttcaagtat ttttgctgct
      241 gacaatgtag atttgtatca agcccctctc aatagcatca ataaataccc tatactacaa
      301 acaccaaaga atgcgattat tttaaagagt tcttctgctg ttattgataa ttcattgcaa
      361 aaattaaatc aaacaaaaga agacaatcaa atgattgttc gttatcagca actgtataaa
      421 ggaatacctg ttattggcgc ccaagtgatg attactaaag gaacagactc aggagtgcag
      481 gccaatgaca atgcagaggt gaacgggcat ttattggata atatagaact taatacgaaa
      541 ccggctatta gtgcgcaaca agcgaaggaa tatgcaaaaa aatcctattt tcaatttagc
      601 ccccaatcta acatacaaca ggaaacagct gaattacaga ttcggccaga ccataataat
      661 caattaaagc tggtttattt ggtttcattt aaaagcgtgc agcaggatgg taaaccagac
      721 tggccttttt ttgttattga tgctcaaaca ggagctttga ttaagcaatg gaacaatatc
      781 aaaaattatt tggatacagg gcctggaggt aatgagaaag ttcaggaata ttggtatggt
      841 aaagatggat tacctgcttt ggatgtgact caaaatggca gccaatgcgt catggaaaac
      901 tcaaaagtca agttggttaa tctccattct caatgggatt gggaaaacac tataaatact
      961 ccttttgaat acgtttgtaa caataatata gaagagaata ttaatggagg attttctcct
     1021 ggcaatgatg cgtattattt cggacatgtt attgttggta tgtacaaaga ctggtatgga
     1081 ctcaatgcct tacaacattc taatggtgct ccaatgcaat tggttatgcg agttcatttt
     1141 gggcaaaact atgataatgc tttttgggat ggacaagcca tgtcatttgg agatgggttg
     1201 gatttttacc cattggtttc tttagatgta gccggtcatg aagtgactca tggttttaca
     1261 gagcagcatt ctggtcttga gtatcatgat caatcaggtg cacttaatga atccctatct
     1321 gatatggcag gacaagcgtc aagagcttat cttttggaaa aaaatcctca gttgtataac
     1381 aaagcttact tacagcccaa tgaagtcaca tggggtattg gtgagacaat agttcgtgat
     1441 tcttatggca aagctttgcg attcatggat tacccctcct ctgatggaag ctctgcagat
     1501 tgtttagaca aaggtattgc tcaaaacaat ggcagctatt gtgctatcag ttatgatgaa
     1561 gtagtagcct atgccaatac acatatcgca cttcctcaag aacgccagag cttcatagtt
     1621 catacagcta gtggtgtgtt taataaggct ttttacttaa tgtctaagga tatgggcatt
     1681 aaaaacgctt atcacatcat ggttgttgct aacacaaaat attggactcc tacgacagac
     1741 tttaaaaatg gagcttgcgg agtcctttat gctgccaggg atttaaatac cgatatcaat
     1801 aaggttaagt ctgcttttgg tcaagtaggt attgatacag ccgggtgtgt tatttagggg
     1861 gagcaaaagc tcccggcaag tttttgctcc aacaaactgc ggtgatctct tgtggttcat
     1921 cgcgagctat ttttgctcgc agtgttgttt gctgggcata gaaattagta tataatcaaa
     1981 caggatcttt cttttagatt tctttggaat agaaacctta ataagctttg attttaaaat
     2041 cattgggatt atatattact ttcgtattag gaacagaatg gcattttttt taatcactct
     2101 ttgcatacta atattgagtt tattctgcct ggccgtaatg gttttaaaat ttattcagca
     2161 gccttccttg cctctatcct tctcccaata tgttaaatta atgtgtagcg gagttattgc
     2221 ttttattgca gatacagtgg gggtcggcag tttcgccgtt aatgtagcct tggcaaaatt
     2281 attaggcact tttcgtgatg atgaattacc tgctgtaaat aatggcgctc aagtcctccc
     2341 tggcgctatc gaatctttat ttttcataca catggtagat gttgatttaa ctacgctact
     2401 tactttagtt accggaacct gtattggtgg gttagtaggt ggttttgtag tgagtcagtt
     2461 aagcaaacaa gccattagac tggcaatgat ttgttcattt gccctaatca tcgttctttt
     2521 aatcgctcat caattgcgtc tattgccagt aggtggtgat ttgacagagt tgcattcctg
     2581 gaagctggtt attggctttt ttgctatggt agtttgtggc tctcttacct ctgtcggagt
     2641 ggggcttttt gtcatggtgc aaggtgtttt atttttaatg aatgtttcac cagttgttgc
     2701 ttttcctatc atgacaactg caggagccat gcaacagccg ctgaccacat tagttttttt
     2761 acaaaaaaat aaaatcccgt taaaaaagac tttgatttta agcttttcag gatgtctggg
     2821 tgttatgatc accattccaa tttttacaaa attaacaata acctggctgc attttttact
     2881 tttattcatc cttatctata attttttagc aattggtcat acttatttac gatcaagacc
     2941 tgtcaaacaa tacgtccaat catctgttaa acttgcggca gcggactgag tctttcagtt
     3001 tataagtttt ctttattgtt tttcgggaac agggctttta aagctatggc taactaatag
     3061 gatgatacag gttgatgttg gtatttgctt gtttttattc aaaattgatt agagtctatc
     3121 cta
//
LOCUS       DBNCWK010000198         3047 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.199, whole genome shotgun sequence.
ACCESSION   DBNCWK010000198 DBNCWK010000000
VERSION     DBNCWK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.199"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..564
                     /locus_tag="AC06IO_002768"
     CDS             <1..564
                     /locus_tag="AC06IO_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HIO9875928.1"
                     /translation="KAKYDPTEPGLQKLPPEIKVMILELLDAKSKLALSQSNYGWRDL
                     IRHLPECTKEITNTLFRLDKKKHRQEIVQLMSGRVTASSMTKLFEELLCFSIPSSYVF
                     LIFFISQKSVALIEVLTVILVFAALTSLAHDLVDYFIESDTKAEKQHAHRRAFQFFAK
                     PSSNAAQQNSEEENLSADPKSWPCEPL"
     gene            830..1585
                     /locus_tag="AC06IO_002769"
     CDS             830..1585
                     /locus_tag="AC06IO_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIO9875929.1"
                     /translation="MYVFRRFSKPIITFLHSLELAFRFLGHLVHSTIKVIFGKLSIVW
                     PNTLEMIYYSGARLVILLSFIGILLGATVSQTVYALLKPFQLHQRVLPIVQNILTHEI
                     LPVLIGFILCIQAALHMVNTRLEHYQENPEAIILAQVLPIIVGMNIASLLLYVYLVFA
                     IFFSFYLTFHFMLGFTNNEFLSYVVSSTTLFDLIYSVVKTLILCIIVGLTSGYYYYEA
                     SIRHIYLRKAVSRILTRGSFWLIIASMYITLTF"
     gene            1653..2339
                     /locus_tag="AC06IO_002770"
     CDS             1653..2339
                     /locus_tag="AC06IO_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIO9875930.1"
                     /translation="MILGGTFFYIEYKRAQKQTFVMFFKGSLKGLVTTAPVTYRGVKI
                     GEVKVIEITENKEHSKVLIPVYVQFFVERTYGFSQDPIHLLIDNGYVANITKPNLLTG
                     VAEIELIKPTPAVKYKQTYYHSYPVFPTHNSAEKYTSMEEAFEAAKKAFEDVSELVRS
                     KEIQDTLEAIQKVSENLGQLASSLNQDVPSVVAYLNQSLKQITSAAYSTQNLTDYLSR
                     YPESLLRGKR"
     gene            2336..>3047
                     /locus_tag="AC06IO_002771"
     CDS             2336..>3047
                     /locus_tag="AC06IO_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HIO9875931.1"
                     /translation="MKNNRHFLKFYCIRDLNWLNSVRASFIYSPFESIASSPFSSYIE
                     LRKKPVKFLLILLIALTLCDCGRSKETQFYLLTPISPQKNSRNSYNHLQIGVDEVSIP
                     DYMKKPQLMIHQTPHHLTLEEFNQWAGALDKNITLVLTTNLSTLMPGVVVQTSPWNNK
                     FHPDYHLQVMISQFEIDIHGNSILRAEYFIYRKEELIYKGNAYYHIKVPLISIETLVQ
                     SMNTNLTHLTEEIARFFIK"
BASE COUNT          927 a          602 c          515 g         1003 t
ORIGIN      
        1 aaagcaaaat acgaccccac agagcctgga ctccaaaagt tacctcctga aatcaaggta
       61 atgattcttg agcttcttga tgccaaatca aaacttgctc tttcacaatc aaattatggt
      121 tggcgtgatt taattcgaca tctgccagaa tgtaccaaag aaataacgaa tacattattt
      181 cgtcttgata aaaaaaaaca tcgtcaagag atagtacaac tgatgtcagg aagagttaca
      241 gcaagttcta tgactaagct atttgaagaa ttactatgtt ttagcatacc ttcgtcctat
      301 gtgtttttaa tcttcttcat atcgcaaaaa tctgtagcgc ttatagaagt cttaactgta
      361 atccttgtgt ttgctgcgct aacctctctc gcccatgatc tggttgatta ttttattgaa
      421 agtgacacaa aagctgagaa acagcatgca caccgccgtg cttttcaatt ctttgccaaa
      481 cccagttcaa acgctgcaca acaaaactcg gaggaagaga atttaagtgc tgatcctaaa
      541 tcctggccat gtgagccatt gtagttcggg tcttggcccg atatttaccg aatgcctcta
      601 cttaattctt cgatgtttat tatatttaca accttatttt gatcatccac tcacccgaat
      661 atcactagca acaaaccaaa cagaatgtta ttcttaggtg tagtaaaaga gcaataagtc
      721 tcactgctat tgagttaaga attaactcct ttttgcctga tgcaagcatc ttgctaagga
      781 agctattatt cttttagcgg cattttgaaa gaggactaat ggattagcca tgtatgtttt
      841 tcgccgtttt tcgaagccta ttattacttt tctccattcg cttgagctcg cttttcgatt
      901 tttaggccat ttggttcata gcactatcaa agttattttt ggcaaactgt ccatcgtctg
      961 gcctaacaca ctagaaatga tttactattc tggagcgcgg cttgttattc tactgagttt
     1021 tattggtata ttattaggcg ctactgtttc acaaacggtt tatgcgctct taaaaccttt
     1081 tcagctacat caacgagtac ttcccattgt gcaaaatatt ttaacccatg aaatattacc
     1141 ggtacttatt ggcttcattt tatgtatcca agccgctttg catatggtta acacccgttt
     1201 ggagcattac caagagaatc ccgaggcaat tatccttgct caagtattac caatcattgt
     1261 aggtatgaac attgcctcct tactgctata cgtttatctt gtattcgcta tcttttttag
     1321 tttttatctc acttttcatt ttatgttagg gtttacaaac aatgaatttt tatcctatgt
     1381 tgtcagctcg acaaccttat ttgatctgat atactcggtc gttaagacat tgattttatg
     1441 catcatagtt gggttgactt ctggttatta ttattatgaa gcatctatta gacatattta
     1501 tttgagaaaa gcagtttctc gaattttaac gcgtggctca ttctggctga tcattgccag
     1561 tatgtatatt actcttacgt tttagggacg acgtgcataa cgaacgattc tatacattag
     1621 tgggtatttt tatagtaggc gccctatgtt taatgattct aggcggtact tttttttata
     1681 tagaatacaa acgcgcacaa aaacaaacgt ttgtcatgtt tttcaaaggc tctttaaaag
     1741 ggctagtcac tactgcccca gtaacctatc gaggcgtgaa gatcggtgaa gtcaaagtca
     1801 tagaaatcac tgaaaataaa gagcacagta aagtacttat tcctgtttac gtacaatttt
     1861 ttgtcgagag aacctatggt tttagccaag accctattca cctgcttatt gataatgggt
     1921 atgtagcaaa tatcaccaaa cctaacctgt taacaggcgt tgctgaaatt gagttgatta
     1981 agccaacccc agcggtaaaa tataaacaaa catactacca ttcttatcct gttttcccga
     2041 ctcacaattc agctgaaaaa tatacatcta tggaagaagc gtttgaggcc gcgaaaaaag
     2101 cttttgaaga cgtcagtgaa ttagttcgtt ccaaagaaat tcaagatact ttagaggcga
     2161 tacaaaaagt ttctgaaaat ttagggcaac tggcctctag cttaaaccaa gatgtgccca
     2221 gtgttgttgc gtatttaaat caaagcttaa aacaaattac cagtgcagct tactccactc
     2281 aaaacttgac ggattatttg tctcgatatc ctgaatcttt acttcgaggc aaaagatgaa
     2341 aaacaataga cattttttaa aattctattg catcagagat ctgaattggc taaatagtgt
     2401 tcgagcctct tttatatatt ctccctttga gagcatcgca tcctcgcctt tttctagcta
     2461 tattgagctt cgaaagaaac ctgtgaagtt cctcttaata ctgctcattg ccttaacact
     2521 ctgcgattgc ggtcgaagca aagaaactca attttattta ttaacaccta tttcccctca
     2581 aaaaaacagc cgtaactcct ataatcattt acagattggt gttgatgaag tgagtattcc
     2641 tgattatatg aaaaaacctc aattaatgat tcaccagaca cctcatcacc taactctcga
     2701 agaatttaac caatgggctg gggccttgga taagaatatt actcttgtat taactactaa
     2761 tctttcaact ttaatgccag gagtagtagt tcagacctca ccttggaaca ataaattcca
     2821 tccggattat catcttcaag taatgatttc acaatttgaa attgatattc atggtaacag
     2881 tattttaaga gccgaatact ttatttaccg aaaagaagag ttaatctaca aaggaaatgc
     2941 ttattaccat attaaagtcc ctttaatcag tattgaaacg cttgtgcaga gcatgaatac
     3001 gaaccttact catttaactg aagaaattgc gcggtttttt ataaaaa
//
LOCUS       DBNCWK010000199         3036 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.200, whole genome shotgun sequence.
ACCESSION   DBNCWK010000199 DBNCWK010000000
VERSION     DBNCWK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.200"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(76..1317)
                     /locus_tag="AC06IO_002772"
     CDS             complement(76..1317)
                     /locus_tag="AC06IO_002772"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIO9875932.1"
                     /translation="MMERQFTDAYIRSLKPRSSRYEEFRDGGFGIRVTPKGLKTFLYR
                     YKINGQRHFITIGHYPAMSLSEAKKRFIDLSDIRHGEVNPKEQIAEQKQKEQSTVKNL
                     ILHWYTNYIEKHRAQPLQIWQHIKVDIIPLLGNKKLENLKTREITQALDKIVGRGSPV
                     HANKVLSTLKQAFNYAVSRGEMLLNPAMTIRARDIGGIEKPRERYLTLDEVKTLWQFL
                     DSEEHGTSFQIRAALKIILLTGVRTGEIRVARWSEFDFENSLWTIPAENTKTNIVMRI
                     HLTEMTKKVLRELQAHSDSEYVITGADGQSILSDRAMPRAVIRIQSRVGIPHWTAHDL
                     RRTFATQLGQTLNVDPVVIEKCLGHKMPKIMATYNKNEMLPQRKEALEQWTSLIKDLV
                     QHQTNDFHFPIELEQISSEIV"
     gene            1655..1891
                     /locus_tag="AC06IO_002773"
     CDS             1655..1891
                     /locus_tag="AC06IO_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HIO9875933.1"
                     /translation="MLADPILCGKYRHYKNKKLYEVIGQARHSETDEEMIVYRALYHC
                     DQFGDNQLWVRPMQMFFENVIHHGKTVPRFQRIE"
     gene            2110..2658
                     /locus_tag="AC06IO_002774"
     CDS             2110..2658
                     /locus_tag="AC06IO_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIO9875934.1"
                     /translation="MRVGYARVSTHEQNLDLQLDELKKAGCEQIFTDKISGSVSERPG
                     LEKVENFLREGDVLVVWRLDRLGRSLKHLIAYVAKLEEKGIGFHSITESINTETSSGK
                     FMFHVFGALSEFERNLIKERTLAGLEAARARGRQGGRPEKLSDEQKELVKTLYANKKH
                     SIQSICKMVGVGKTTLYKYINN"
     gene            2718..>3036
                     /locus_tag="AC06IO_002775"
     CDS             2718..>3036
                     /locus_tag="AC06IO_002775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875935.1"
                     /translation="MDEIDEFKKKLEEYKKQVMEASFTNLNKLVEKYGSDINDNWVFM
                     YLTAAFISKHEKQWGEDKYEKELSPINNAIDAILEKHGLECVEEFNPGDKPPEIDKLL
                     KEYD"
BASE COUNT          908 a          493 c          650 g          985 t
ORIGIN      
        1 aagagagttt cttcagcaac tcaaaaaata ggtataattt ataccaattt tatacctatt
       61 ctatacttat ttgcgttata caatctcaga agatatttgc tctaattcaa ttggaaaatg
      121 aaaatcatta gtttgatgtt ggaccaaatc cttaattagg ctagtccatt gttcaagggc
      181 ttcttttctc tgtggtagca tttcattttt attataagta gccataattt taggcatctt
      241 atgtcccaaa catttttcaa tcacaaccgg atctacattc aatgtttgcc ctaattgagt
      301 tgcaaaggtt cggcgaagat catgcgctgt ccagtgcgga attccgacac gggattgtat
      361 tctgattacg gctcttggca tagctcggtc ggataaaata ctttggccat ctgcacctgt
      421 aatgacgtat tctgaatcgc tgtgtgcttg gagttctcgc aatacttttt tggtcatttc
      481 tgtgaggtga attctcatga ctatgttggt tttagtattt tcggcaggga tagtccataa
      541 ggagttttca aaatcgaatt ctgaccatct agctacgcgg atttcgccag ttctaactcc
      601 tgtaagcagg atgattttca gggctgctct gatctggaag gaggtgccgt gttcttcgct
      661 gtcgaggaat tgccataggg tttttacttc atcgagggtt aaatagcgtt ctcggggttt
      721 ttctataccc cctatgtctc ttgcgcgtat agtcatggct gggtttaaca gcatttcgcc
      781 tctgctaact gcgtagttaa aggcttgctt tagtgtgctt agtactttgt ttgcatgaac
      841 aggggagcca cggcctacaa ttttatcgag tgcttgcgta atttcacgtg ttttaaggtt
      901 ttccaatttt ttattgccca agaggggaat gatatccact ttgatgtgtt gccagatttg
      961 cagtggttgg gctcggtgct tttcgatgta gttggtatac caatggagta tcagattttt
     1021 taccgtgctt tgctctttct gtttttgttc tgcaatctgc tctttggggt ttacctcgcc
     1081 atggcgtatg tcgcttaaat cgataaaacg ttttttggct tccgataaac tcatagcagg
     1141 gtagtgacct atggtgatga aatgcctttg gccgttgatt ttataacggt atagaaatgt
     1201 ttttaaccct ttgggggtga ctcggatacc aaaacctcca tctctgaatt cttcatagcg
     1261 agaagatctt ggtttgagcg atcgaatata agcgtctgta aattgtcttt ccatcattgc
     1321 accacttaaa aattaggtgt acttctaggt gtactttcgt gagatctcac atgaaatcgt
     1381 ttgatatttt ttgaagtata gcagatggta aaaattaata aaatacaata agttaatgat
     1441 gctttttgat atcaattgat accgtttgaa aacggacttt ttaagaatgg ggtgctagtg
     1501 gtcgaaggtt caaatccttc cgtcccgacc aagcccagta agggttttat caaagtctcc
     1561 aaaccccgtc agaaatattt ggtaccagtt ttggtaccga ttgtaaatag tactagctag
     1621 tcagtgaaaa ctaatgattc acgtgagagt gtaaatgtta gctgatccta ttctatgtgg
     1681 aaaatacagg cattataaaa acaaaaaatt atatgaagtc attggccaag ctcgtcatag
     1741 tgaaacagat gaagagatga tagtgtatag ggcactttat cattgtgatc aatttggtga
     1801 taaccagctt tgggttcgcc ctatgcaaat gttttttgag aatgtcattc atcatggaaa
     1861 aacagttcct cgatttcaac gaatagaata gtattaattt aattacttat gctagctaat
     1921 tgagttgctg aagtaaggca aaacgaaccc atttgtcgtt atgattcgta aaatgatggg
     1981 tttgtgaacg tctaacaagg atgtttgaaa attcaaaaat caccccgaaa accagttcga
     2041 aaactctata cattaatcaa agttcatttt aatctattct ttagtctttc tgaacaacaa
     2101 ggaataataa tgagagtagg gtatgccagg gtttctacgc atgaacaaaa tttagacttg
     2161 caacttgatg aattaaaaaa agcaggttgt gaacaaattt ttacagacaa aataagtggt
     2221 tccgtatccg agcgtcccgg tttagagaaa gtagaaaatt ttcttagaga gggagatgta
     2281 ttagttgttt ggcgattgga tagactaggg cggtctctta agcatctgat tgcatatgta
     2341 gctaagcttg aggaaaaagg gatagggttt catagcatta ctgaatctat taatactgaa
     2401 acatcatctg gtaagtttat gtttcatgtc tttggcgcac tttcagaatt tgagcgtaat
     2461 ttaattaaag agaggactct tgccggtctt gaggcagcga gagctagagg tcgccaaggt
     2521 ggtaggcctg agaaacttag cgatgagcaa aaagaactgg ttaaaacact ttacgctaat
     2581 aaaaaacact ccattcagtc gatttgcaag atggttggcg tgggaaagac tacactatat
     2641 aaatacatca ataattagag aaatattctt acattgtgta atcaatcgtg ctgaggatgg
     2701 tatgtgaagg agtcttaatg gacgagatcg atgaatttaa aaaaaaatta gaagaatata
     2761 aaaagcaggt gatggaggct agctttacga atttaaataa attagttgaa aaatacggtt
     2821 cagatataaa cgataattgg gtttttatgt atctcacagc agcttttatt tccaaacatg
     2881 agaaacaatg gggggaagac aaatatgaaa aagaattatc acctataaat aatgctattg
     2941 atgctatatt agaaaaacat gggcttgaat gtgttgaaga atttaaccca ggtgacaaac
     3001 caccagaaat agataagttg ttaaaagagt atgatg
//
LOCUS       DBNCWK010000200         3029 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.201, whole genome shotgun sequence.
ACCESSION   DBNCWK010000200 DBNCWK010000000
VERSION     DBNCWK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.201"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            170..>3029
                     /locus_tag="AC06IO_002776"
     CDS             170..>3029
                     /locus_tag="AC06IO_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HIO9875936.1"
                     /translation="MLKFHSDPGAKMIKKLLIKVEKLINNLPKELKLSETILSSIKED
                     INSLASRFDGSKGYINYYSPKVVQQINEKQKLLEKYQNYLTALLAVLICSKQSRDIRD
                     NKKLEPVSEITDLKNLLKHKDVEKIINDITQFDEDQRELKRIERLIQKDEEKEDNKKM
                     HLGKRSKLELRRAELSKRVQLFNNKLKNIPQAEEIKAVLDYCQKYIEKHINLSKQLPK
                     GMLDVKRALSLFDEINSTKTQIRKLSKDKLDSSGQIKLEGLKKELTKAELALQFSYSE
                     EEDKTIKDMLNDLKRLMKMSIRDYQKLATSGGEINVFLHKTTGFYWESLFLYRNVLCE
                     INEYLKKNKKKLSEQGMLVAECISKAFSQMPELDTIEKALEKVKRIDDDLKRQEEYNE
                     KINGTWIRFYKKGPVKFVQQVLEQNLNEKIFYIPYNPHNYVLDHPAQSDFADTLGNCY
                     GETQMFLKQINSKSPAINNICPERVLINYQLDQSRTIAKNTKKEKIGIFEADEKTKAQ
                     VTWDGIKDILTSKVTSTAFGDVCMITLTGAQVSGREKLVGHGIGLIKMKNPAPYKYVV
                     YDYAFGGAMGFSSDTQLELFFKQILEGEGSYVPFKKCLVEKVGEVSKECQQLINGSPG
                     IASLTQKSLDKTCNRDFWDKARISFLIKYYPIGTDENRILDLIEKLPQSKDRIELYEQ
                     LVKEPKIGIGNLVKCIICSGKISYLANLLTSDLLAVDITNKIKFKLNEQAKKEIVALI
                     NNDQIEPNITCKLFSNNQTIVLATYKKDKNSLQYADIDPVVTLLQGKNTITPSDACRA
                     FPKAEKIIIAAYKSDPSSLQFANLGLVQQLLRKGSINLEHASKAFTENSQFKEANEAY
                     QLLKSLKLLPKNEKETEKAKRIELLKSIDQKLEITNLRQELYPLISNMCIRLLDDIRI
                     LSPEDTIIKNSRLSKLISSNQCDINTLKTLENKKMELTK"
BASE COUNT         1166 a          459 c          536 g          868 t
ORIGIN      
        1 agtcagagga ctgacatcac ttgattctac aggtgtttag tttaatcact atcattaaac
       61 gggtatatcg tgattttctg gttttttgta aaccaaggaa atagacgatc ccgtaattcc
      121 ttggttttga aaactttatt actagaaatt tcagttaaat gtaactgatt tgctaaaatt
      181 tcattcagat ccaggggcaa aaatgattaa aaaattgtta attaaagtcg aaaaattaat
      241 taataatctt ccaaaagagt tgaaattatc tgaaacgatt ttatcctcca ttaaagaaga
      301 tataaatagc ctggcaagtc gttttgatgg gtcaaaaggt tatattaact attattctcc
      361 taaagtcgta caacaaatca atgaaaaaca aaagttactt gagaaatacc aaaattattt
      421 aactgcatta cttgccgttt taatttgctc caagcaaagc agagacatta gagacaataa
      481 aaaacttgaa cctgtttctg agattaccga tttgaaaaat ttattaaaac acaaggatgt
      541 cgaaaaaatt atcaacgaca ttactcagtt tgatgaagac caaagagaac ttaagagaat
      601 tgaacgtttg atacaaaaag atgaggagaa agaggataat aaaaaaatgc atcttggtaa
      661 aagatccaaa ttagagctta ggagggcaga gctttctaaa agagttcaat tgtttaataa
      721 taagttaaaa aacattccac aagctgagga aatcaaagcc gttttagatt attgtcaaaa
      781 atacatcgag aaacatatca accttagcaa gcaattacca aaaggaatgc ttgatgttaa
      841 acgagcattg tctttatttg acgagataaa ttccaccaag acccaaatta ggaagttaag
      901 caaagacaaa ctggattctt cagggcaaat aaaacttgaa ggtttaaaaa aggaactcac
      961 aaaagctgaa ttggctttgc aatttagtta ttcagaggaa gaagataaaa cgatcaaaga
     1021 catgctcaat gatttgaaaa ggcttatgaa aatgagcatc agggattatc aaaagttagc
     1081 cacatctggc ggagaaataa atgttttttt gcataagaca actggttttt attgggaaag
     1141 cctttttctg tacagaaatg ttttgtgcga aattaatgaa tatcttaaaa aaaataaaaa
     1201 gaaactgtct gaacaaggaa tgctggtagc tgaatgcatt tctaaagcat tcagccagat
     1261 gcctgaattg gataccattg aaaaagcttt agaaaaagtc aaaagaatag atgatgatct
     1321 taaaagacaa gaagaatata acgaaaaaat caatggaact tggattaggt tttataaaaa
     1381 aggtccagta aaatttgttc aacaagttct tgaacagaac ttaaatgaga aaatttttta
     1441 cataccgtat aatccgcata attatgtgct ggatcatcct gcccaatctg atttcgccga
     1501 taccttagga aattgctatg gcgaaactca gatgtttctc aaacaaataa acagtaaatc
     1561 tcctgcaatt aataatattt gtcctgagag agtattaatc aattatcaac tagatcaatc
     1621 caggacaatt gctaaaaata caaaaaaaga aaaaatagga atatttgagg cagatgagaa
     1681 gacaaaagca caagtaacat gggatggaat taaagatatc ttaacatcaa aagttacctc
     1741 taccgctttt ggtgatgtgt gcatgatcac tttgacggga gctcaagttt ctggtcgtga
     1801 aaaattagta ggtcatggta ttggccttat caaaatgaag aatcctgctc cttataaata
     1861 tgtggtatat gattatgctt ttggcggtgc aatgggattc agcagtgata ctcaattgga
     1921 gctattcttt aaacaaatcc ttgaagggga gggatcctat gttcctttta agaaatgtct
     1981 ggttgaaaaa gtaggagagg tgagtaaaga atgtcaacaa cttattaatg gtagtcctgg
     2041 tattgcttct ctgacacaaa aaagtcttga taaaacatgt aatagagatt tctgggataa
     2101 agcccgcatc agctttttga taaaatatta cccaattggc acagatgaaa acaggatact
     2161 tgatttaata gaaaagttac cacagtctaa ggatagaatc gagctatatg aacagcttgt
     2221 taaagagccc aaaattggta tcggtaacct ggttaagtgc atcatttgct caggtaaaat
     2281 aagttacctg gcaaatcttt taacatccga tttattagct gttgatatta caaataaaat
     2341 aaaatttaag ttgaatgaac aagctaaaaa agaaatagtg gctttaatta ataatgatca
     2401 gattgaacca aacataactt gtaaactatt ctctaacaat caaacgattg ttttggcaac
     2461 ctataaaaag gacaaaaact cactccaata tgcggacata gatccggttg taacgctttt
     2521 gcaagggaag aataccataa caccttcgga tgcctgtcgg gcttttccta aggcagaaaa
     2581 aataatcatc gcagcctata agtccgatcc atcatcgttg caatttgcta atctcggttt
     2641 ggttcaacaa ttattacgaa agggttctat taatctcgag catgcttcta aagcttttac
     2701 agagaactca caattcaagg aggccaatga agcttatcag cttttaaaat ctctgaaact
     2761 tctgccaaaa aatgaaaaag aaactgagaa agccaagaga atcgagttat taaaatcgat
     2821 tgatcaaaag ctggaaatta ctaatttgag acaagaactt tatcccttaa tttcaaatat
     2881 gtgtattcga ttacttgatg atattcgaat cctatcccct gaagatacaa taatcaaaaa
     2941 cagcagattg tccaagttaa tcagttcgaa tcagtgtgat atcaatacat taaaaacact
     3001 tgaaaataaa aaaatggaat taactaaaa
//
LOCUS       DBNCWK010000201         2994 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.202, whole genome shotgun sequence.
ACCESSION   DBNCWK010000201 DBNCWK010000000
VERSION     DBNCWK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.202"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            63..530
                     /locus_tag="AC06IO_002777"
     CDS             63..530
                     /locus_tag="AC06IO_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875937.1"
                     /translation="MMKTKTESSFLYKGLGFPIELEHVAMICINGEWMPKIDVRKIAD
                     EAIKKLAIQEERLTGNQVKFIRSYFSMTLREFGKTVVHESHTAVNKWEKCGNDITNMN
                     ENTEQVLRIYIIEQTQTKTQAEQENFYSNFKKSKVFVNAERKNPKPIRLNLCA"
     gene            1211..1915
                     /locus_tag="AC06IO_002778"
     CDS             1211..1915
                     /locus_tag="AC06IO_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HIO9875938.1"
                     /translation="MDFLFVTTYELDALSEIPLMQRVVYLMGIRPYMDRKTFIVGIKR
                     KISYQSLRETLYVAPIKGVKTGCPSHQQMKRVIKSLARQGIIEIRSTAQNLIVKCLLA
                     NPYEPIEIENESQPKPNPSKQAEQNQPLPSENLKSIKPEKSSYSKSGFKAQPSSNLKE
                     LYQKFEQFWQLYPLPSYKNEAWKQFQQIDPDDRLFSQIMDGLQKQLTKINRLGAYWPY
                     PASWLGQKSWFENSHK"
     gene            complement(1956..2933)
                     /locus_tag="AC06IO_002779"
     CDS             complement(1956..2933)
                     /locus_tag="AC06IO_002779"
                     /inference="COORDINATES: protein motif:HMM:NF019371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIO9875939.1"
                     /translation="MSHYNKPYLTFEQQLELLKSRGLEVTNDKVALEYLGRLGYYRLS
                     GYWYPCRKLLPLVVQQTKSVRPRRLDEFLPGSRFQDVVDLYVFDKKLRLLILDAIERI
                     EVAFRVDIAYLLGERDPFAYTNPDLLHGHFTKKIDPNTGKTKYQEWISKHEQLINRSK
                     EDFVEHYKVKYGLPLPIWVAIELWDFGLLSVFYKGMAVKDKAIIANKYDIPDWQIMES
                     WLRSLNYVRNVAAHHSRLWNRNLIDQPKLAKPGAMLAFDSFIDNVHMTSRIYVVLCIL
                     AHYMKIICPRSTWQMRLVELIHSFPNSNYVSIQDMGFPAEWEQHLFWKN"
BASE COUNT         1065 a          629 c          478 g          822 t
ORIGIN      
        1 tataattaat tgtgttgtat gctctaggca actttgatca tgagtattta caaagtagaa
       61 atatgatgaa aactaaaacc gaatcatcgt ttttgtataa aggcttagga tttcccatcg
      121 aattggagca tgtcgcaatg atctgtatca acggagaatg gatgcccaaa attgacgttc
      181 gtaaaattgc cgatgaagcc attaaaaaac ttgccattca agaagagaga ttaactggca
      241 accaagttaa attcattcgc tcctattttt ccatgaccct tcgagagttt ggaaaaacag
      301 tggttcatga atcgcacacc gctgtcaata aatgggaaaa atgcggtaat gatattacca
      361 acatgaatga aaataccgag caagtgttac gaatttacat tattgaacag acacagacta
      421 agacccaagc cgaacaagag aatttctatt caaattttaa aaaaagcaaa gtatttgtga
      481 atgctgaacg caaaaatccc aagccaattc gattaaatct ctgtgcataa cggctcaccc
      541 ctgctctaat caaaatcaga caaagtctta ccaaatttaa ataaatggta agactttgtc
      601 tgattttgat taaattaaac cgtgacaatt tgtcatagtt tgaaaataac gtcatatatg
      661 ttcaatcaat aacatgtaga aaaagccttt gacaaacacc cagatttaaa taaacatcat
      721 caatagataa ccattaaact aaactttcac cgcaattttt ttaactccca aaatcaatga
      781 gcctaaatcc gtccaaaaca aggccgacct taacccgaca catcaacccg acacaaaccc
      841 gacatagcaa gattcagtac tgacaagaag aaaaaacctg tcgaatagaa aaacccgaca
      901 aagagaaatt ctctaggccg acccacctca taactccaaa atctgttttt tatgttttta
      961 cgcgcgcgag gaaaaatttt ttaaacaatt ttgaacaaaa ataaccaatt accaatgcaa
     1021 aaacgtctca gtaaaaaaat ataaggttca ttgaatatca tttagtatca agctcgtttt
     1081 ccctattgac actcttcacc aatttcctag aatgaatcaa gtttaatgca tcttggttat
     1141 ctaaacgtca tcgattgata gcgtttgata tcaattgaaa taaaaataca gggttaaaac
     1201 aggagtaaaa atggatttct tattcgtcac cacatacgaa ctggacgcac tcagcgaaat
     1261 ccccctcatg caaagagtag tctatctcat gggtatccgc ccctacatgg acagaaagac
     1321 attcatagtc ggcattaaac gaaaaatcag ttaccagtca ctgagagaaa cactttatgt
     1381 tgcaccaatc aaaggcgtaa aaacgggctg tcctagtcac caacaaatga aacgcgtaat
     1441 taaatctctg gcacgccaag gcatcataga aattcgctca acagcccaaa atctcattgt
     1501 gaaatgtctt ctggcaaacc catatgagcc gatagagatt gaaaatgaat ctcagcctaa
     1561 accaaatcca tcaaagcaag cagaacagaa tcaaccatta ccatcagaaa atctgaaatc
     1621 aatcaaaccc gaaaaatcat cctactcaaa atcaggcttt aaagcgcagc ccagcagtaa
     1681 cctaaaagag ctataccaaa aattcgaaca attctggcaa ctctaccccc tccctagcta
     1741 taaaaatgaa gcatggaagc aatttcaaca aattgatcct gacgatcgtc ttttttcaca
     1801 gatcatggat ggattacaaa agcagttaac caaaattaat cggctaggag cttattggcc
     1861 ttatcctgct agttggttgg gtcaaaaaag ttggtttgag aattcacata agtagttaaa
     1921 gccgcctaag tctatatgca ctccacaaaa tcaattcaat ttttccagaa taaatgttgt
     1981 tcccattcag ctggaaatcc catatcttga atacttacat aattggaatt tggaaaggag
     2041 tgtatcagct ccactagacg catttgccat gttgatcgcg ggcaaattat tttcatgtaa
     2101 tgcgccaata tacataaaac cacatatatt ctcgatgtca tatgaacatt atcaatgaat
     2161 gaatcgaaag caagcattgc acctggcttt gcaagcttcg gctgatcaat taagtttcta
     2221 ttccataaac ggctgtggtg cgccgctaca tttcggacat aatttaaaga acgtaaccaa
     2281 ctctccataa tctgccagtc aggaatgtca tatttgttag caatgatagc cttatcttta
     2341 actgccattc ctttataaaa tactgacaaa agaccaaagt cccataactc aatagcaacc
     2401 catatcggca atggtaaacc gtactttacc ttgtagtgct ctacaaaatc ttcttttgag
     2461 cgatttatta attgctcatg tttagaaatc cattcttgat acttcgtttt acccgtattt
     2521 ggatcaattt ttttagtaaa atgaccatgt aataaatcag gattggtgta ggcaaacggg
     2581 tctctttcac caagcaaata agctatgtcc actctgaaag caacttctat tctctcaatt
     2641 gcatccaata ttaataggcg cagcttttta tcaaagacat acaaatccac aacatcctga
     2701 aatcgagaac ctggcaaaaa ctcatctaaa cgccgaggtc ttacagattt tgtttgttgt
     2761 actactaaag ggagtaattt acggcaggga taccaataac cacttaaacg atagtagccc
     2821 aatcgaccta gatactccaa tgccactttg tcattggtta cctccaaacc acgagatttt
     2881 aataactcaa gttgctgttc aaaggtaaga taaggtttat tataatgcga cattttttag
     2941 gctttataaa aaagaaaacc cgctcgtgca tttttcaatg tagaggagca ggcc
//
LOCUS       DBNCWK010000202         2932 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.203, whole genome shotgun sequence.
ACCESSION   DBNCWK010000202 DBNCWK010000000
VERSION     DBNCWK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.203"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            2..217
                     /locus_tag="AC06IO_002780"
     CDS             2..217
                     /locus_tag="AC06IO_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HIO9875940.1"
                     /translation="MIGINSELLLNEIRQIVINHFPTFENKHLTHKTSEQNKYLAITV
                     TVYAENQISLDGLYQDLSQHPAVKMVL"
     gene            217..816
                     /gene="lipB"
                     /locus_tag="AC06IO_002781"
     CDS             217..816
                     /gene="lipB"
                     /locus_tag="AC06IO_002781"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIO9875941.1"
                     /translation="MIIHNLGIKDYTEIWEQMKEFTAVRDSNSYDELWLLEHHPVYTQ
                     GQAGKPEHVLNPNSIKIVQSDRGGQVTYHGPGQLVAYVLMDIRRRNLGIRTLVAKLEE
                     ILISVLEYYRIPANIRSGAPGVYVGEKKIASIGLRVKNGCTYHGIALNVNMDLSPFLG
                     INPCGFAKMEMTQMSHFHPNIQLEEVSQHFVQYFLTQFK"
     gene            826..2862
                     /locus_tag="AC06IO_002782"
     CDS             826..2862
                     /locus_tag="AC06IO_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIO9875942.1"
                     /translation="MNLLADYVQPLTYWLQANPHWSLFITFLIALSESLAVIGSIIPG
                     SVTMTAIGILAGSGIMRIDLTLLAATLGAICGDSLSYFLGYFYSDRLIEIWPFRKYPG
                     LLKYGKDFFASHGGKSVLIGRFVGPLRSIIPVIAGIMHMKQWRFLIANIVSAIGWSIL
                     YVMPGVLIGAAGHELSAEGATRFFILILVILAVIWLSSLVLKWLFIRLNSFLRSNLHR
                     FWLEIKTSPRLSKVYYAITPVQEENHYNTVGLLLIALVIFFLFLTLVIFTIEGQLLDY
                     INVPVHFFMQSFHSSLLEAVFIVCAQLTSKSTLTGLFVASCIWFIYHKNYRATVYLSS
                     ITLLSGVIALFLTKIIDSPRPQGLLVTLPGSSFPSVSISIATALYGFVLFYINSKYSL
                     ITSSFKTIILIVLGLSGFANIYLGDFWFSDILAAYLLGAEVCLIHCLVYRRFSTSVIK
                     NEHPLIMLISLLMVIVFATAVSTVVNFKEQVHDHMPYHKEFTLTEKQWWDQQRPLLPL
                     YRLSRIGKRISLLNIQYVGDLDALQNKLEQFGWEPQSDKTFFTRLLMRMNNAPSNVKL
                     PLLTQLYENKKPGLIMTYQDKKSKLIFDLTIWQSNYYLSEPKRIVWIGSIHQNIFNLK
                     QKKSRHNLPPVLNPLSYVIPALDDFTLRRIMLSDDLVKTSVFPTAPYILLIKTK"
BASE COUNT          879 a          463 c          567 g         1023 t
ORIGIN      
        1 aattatcgga attaactcag aattattgct caatgaaatc aggcaaattg ttattaatca
       61 cttccctact tttgaaaata agcatttaac tcataaaacg agtgaacaaa acaagtactt
      121 ggctattact gtgacggtgt atgcagaaaa tcaaatctcg ctggatggat tatatcagga
      181 tttaagtcaa catcctgctg tgaaaatggt tttataatga taattcataa tttaggcata
      241 aaagattata ctgaaatatg ggagcagatg aaggagttca ctgcagtccg cgacagtaat
      301 tcttatgatg agctttggtt attagagcat catcctgtat atactcaggg gcaggcaggg
      361 aaaccagagc atgtacttaa tcccaactct ataaaaattg tgcaatcaga tcgcggcgga
      421 caagttacat atcatggccc aggtcaacta gtagcctatg ttttaatgga tattcgccgc
      481 agaaacctgg gcataagaac tttagtagca aaactggaag aaatattaat ttcggtttta
      541 gaatattata gaattccagc aaacattcgc agtggggcac ctggagttta cgtaggtgaa
      601 aaaaaaatcg catcgatagg actgagggtg aaaaatgggt gtacctatca tggtatagca
      661 ttaaatgtga acatggattt gagtccattt ttaggcatta acccatgtgg ttttgctaaa
      721 atggaaatga cacagatgag tcatttccat cctaatattc agttagagga agtaagccaa
      781 cattttgtac agtatttttt gactcaattt aaataatagg aaattatgaa tttgttagca
      841 gattatgtgc aaccactgac ttattggcta caagcaaacc cacattggtc cctgtttatt
      901 actttcttaa tcgctttaag cgaatcttta gctgtcattg ggagtattat acctggctct
      961 gttaccatga ctgcaatagg gatattagca ggttctggaa tcatgcgtat tgatttgaca
     1021 ttgcttgctg ctactctggg agctatttgt ggagatagcc ttagttattt tttgggatat
     1081 ttctatagtg atcgtttaat tgagatttgg ccttttagaa aatacccggg cttattgaag
     1141 tatggtaaag atttttttgc aagccatggc ggtaaaagcg tattaattgg tcgttttgtt
     1201 ggtccattgc gttctataat tccagtaatt gctggaataa tgcatatgaa acagtggagg
     1261 tttctgatcg caaatatagt ttctgctatc ggatggtcta ttttatatgt tatgccaggg
     1321 gtgctgattg gcgcggcagg acatgaatta tcagcagagg gagctacaag gttttttatc
     1381 ttgatattgg tgattcttgc tgttatttgg ttgagtagcc tcgttctcaa atggttattt
     1441 attagattaa attcattttt gagaagtaac ttgcatcgtt tttggttaga aattaagaca
     1501 agccccagat tatctaaagt gtattatgct atcacccctg ttcaggaaga aaatcattac
     1561 aatacggttg gtttattact tattgctctc gtgatttttt ttctcttttt aacgttagtt
     1621 atttttacca ttgaaggtca actgctagac tatattaatg tgcctgtgca tttttttatg
     1681 caaagctttc acagttcctt actggaagca gttttcattg tttgtgctca attaacaagc
     1741 aaatcaacct taactgggtt atttgtcgct tcttgtatat ggtttattta tcataaaaat
     1801 taccgagcta cggtttattt aagcagcata accctattga gtggtgttat tgctttattt
     1861 ttaactaaaa ttattgatag ccccagacct caagggctat tggttacttt gcctggttcc
     1921 tcttttccat ctgttagtat ttctattgca actgcattgt atggttttgt tttattttac
     1981 ataaatagta agtattcctt aataaccagc agttttaaaa caattattct aattgtgctc
     2041 ggattaagtg ggtttgctaa tatctattta ggtgattttt ggttttctga tattttagct
     2101 gcttatctgt tgggagctga agtatgctta attcattgtt tggtatatag aaggtttagt
     2161 acttctgtta taaagaatga acatccttta attatgctca tatcactgct tatggttatt
     2221 gtatttgcga cagctgtttc aacggttgtg aattttaaag agcaggttca tgatcatatg
     2281 ccctatcaca aggagtttac tttaacagaa aaacaatggt gggatcagca aagacctctt
     2341 ttacctttgt acaggttaag cagaattgga aaacgaataa gtctcttaaa cattcaatac
     2401 gtaggtgatc tggatgcatt gcaaaataag ttagaacaat ttggttggga gccacagagc
     2461 gataaaactt tttttaccag attgctgatg agaatgaata atgcaccatc aaacgttaaa
     2521 ctgcctttac tcactcaact atatgaaaat aaaaagccag gcttgataat gacgtatcaa
     2581 gataaaaaat caaagttgat ttttgattta actatttggc aatcaaacta ttatttatct
     2641 gagcctaaaa gaattgtgtg gataggctcc attcatcaaa atatttttaa tttaaagcaa
     2701 aaaaaatcca ggcataattt accaccggta ttaaatccat tgtcttatgt aatccctgct
     2761 cttgatgact ttacccttag aaggattatg ctatctgatg atttggtaaa gacatcggtt
     2821 ttccctactg ccccctatat tcttttgata aaaactaaat agcagagcgt cattgattgc
     2881 aggaagcatg ttctgattta tttatattag gcctggctag taacccatgt aa
//
LOCUS       DBNCWK010000203         2932 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.204, whole genome shotgun sequence.
ACCESSION   DBNCWK010000203 DBNCWK010000000
VERSION     DBNCWK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.204"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            136..1767
                     /gene="proA"
                     /locus_tag="AC06IO_002783"
     CDS             136..1767
                     /gene="proA"
                     /locus_tag="AC06IO_002783"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ProA"
                     /protein_id="HIO9875943.1"
                     /translation="MHPNYYLSPLAVAIALGIASPVKAADPIPLQKSSFSEVTQKFQL
                     TLPGVMKGAVVSTNSLQFIRQHTDGNKVTHVRMQQQYAGFPVFGGYAILHSKNATPGL
                     ATAKSDVKMNGVIYDGLQTELGQPKPSFVKNASLALQQFKDKYANKQISEDQVTPMIY
                     IDEKHQAHWAYKVSVLVIHDDRIPERPTAIIDAETNKPFVQWDDVKTEKVQAKGMGFG
                     GNRKIGEYQFGKDLPLLEITRDSSVEMCFMENTDVKVVDMGHKYYSNNKPMQFTCKET
                     PDTQSTKTYYTGYSADGYDRDNGAASPTNDALYAGYVIKHMYHDWYGVEALTKSDGSP
                     MQLVMRVHYGQGYENAYWDGKQMTFGDGDTMMYPLVSLGVGGHEVSHGFTEQHSGLEY
                     FGQSGGMNESFSDMAAQAAEYYSVGKNSWQIGPEIMKEDSGYDALRYMDKPSRDGMSI
                     DVADDYYGGLDVHYSSGVYNHLFYILANQPNWNLRMAFDVMVKANMDYWTPYSTFDEG
                     GCGMLSAAKDLGYNLDDIKKSLSEVTINYQSCYVD"
     gene            complement(1769..2617)
                     /locus_tag="AC06IO_002784"
     CDS             complement(1769..2617)
                     /locus_tag="AC06IO_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIO9875944.1"
                     /translation="MNTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDDIAE
                     HLQNDYYFIAVDLPGHGHSSHLPPGCIYHFTDGIFTVVEIINALGLNKLHLLGHSMGA
                     CLGSLVAGVAPDRFLSLSLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNKF
                     EHAALARSVKGYVSLDIAKSLCERGIQQENGLYYWRHDRRLLAPSPLQMTETQVLSCL
                     TEIKAQTYLIWASKGFSFDSDKMKARIQAVKNIKIERLDGGHHIHMEKPEVISRLLAK
                     FYQSIK"
BASE COUNT          905 a          501 c          616 g          910 t
ORIGIN      
        1 ggatcatcct taattttgca gttgagcatt agtctacgct aaaaaattaa ctgaaaaatt
       61 ataggggaat ataacaaacc tgggcactcc aggaagagtt gctgtatgag aatttagttt
      121 aaggagttag aatcaatgca cccaaattat tatttgtcac ctttagctgt agctatagca
      181 ttagggattg cttcacctgt aaaggcagcc gacccgatcc cgttacaaaa gtcgtctttt
      241 tctgaagtca cacaaaaatt tcagttgact ttgcctggtg ttatgaaagg tgctgtagtt
      301 tcaacgaata gtttgcagtt tataagacag catactgatg ggaataaagt gacgcatgtt
      361 cgtatgcagc aacaatatgc cggatttcca gtttttggtg gttacgctat cctgcacagt
      421 aagaatgcca ctcctggttt agcgacggca aaatctgatg tgaaaatgaa tggtgtgatt
      481 tatgatgggt tgcaaacaga attaggccag cctaaacctt catttgtgaa aaatgcttct
      541 ttggcattac aacaatttaa ggataaatac gcgaataagc aaattagtga agatcaagta
      601 accccaatga tttacattga tgaaaaacat caagctcatt gggcttacaa agtcagcgtt
      661 ttagttatcc atgacgatag aattcctgag cggccaacag caattattga tgcagaaacc
      721 aataagcctt ttgttcaatg ggatgatgta aaaacggaaa aagttcaagc taaagggatg
      781 ggctttggtg gaaatcgtaa aattggtgaa tatcaatttg gcaaggactt gccattatta
      841 gaaatcacac gtgattcaag tgttgaaatg tgctttatgg aaaataccga tgttaaagta
      901 gtcgacatgg ggcataagta ctattcaaat aacaaaccta tgcaatttac ttgtaaagag
      961 actcccgata ctcaatcgac taaaacttat tataccggat attcggctga tggttatgat
     1021 agagataatg gagctgcttc tcctaccaat gacgcattgt atgcaggtta tgtcattaaa
     1081 catatgtacc atgattggta tggggtggag gcattgacta aatcagatgg atcgccaatg
     1141 caattagtta tgagagtgca ttatggtcaa ggctatgaaa acgcctactg ggacggcaaa
     1201 caaatgacct ttggtgatgg tgacaccatg atgtatcctt tggtttctct aggtgtaggt
     1261 ggtcatgaag tcagtcacgg gttcaccgaa caacattctg gacttgaata tttcggtcag
     1321 tctggtggca tgaatgagtc gttttcagat atggctgccc aagctgctga atattattca
     1381 gttggaaaaa acagctggca aatcggcccg gaaataatga aagaagacag tggttatgac
     1441 gcattgcgtt atatggataa accaagccgt gatggtatgt ctattgatgt agctgatgat
     1501 tattatggcg ggttagatgt tcattactcc agtggagtgt ataaccattt attctacata
     1561 ttagccaatc aacctaattg gaatcttcgt atggcttttg atgtcatggt gaaagccaat
     1621 atggattatt ggacacctta ttcaaccttt gatgagggtg gttgcggtat gttgagtgct
     1681 gccaaagacc tgggttacaa tctggatgat attaagaagt cattaagtga agtgactata
     1741 aattatcaat cttgttatgt cgattaattc attttattga ttgataaaat ttagccagaa
     1801 gtcgactgat gacttctggc ttttccatat gaatatggtg tccaccatct aacctctcaa
     1861 ttttaatatt ttttactgct tgaatccttg ctttcatctt atccgaatca aatgaaaatc
     1921 ctttgctggc ccaaataagg taagtttgag cttttatttc tgtaaggcag gaaagaactt
     1981 gtgtttcagt catttgtaat ggagatgggg ctaatagcct gcgatcgtgt ctccaataat
     2041 atagaccatt ttcctgttga attccgcgtt cgcaaagtga cttggcaata tctagagaaa
     2101 catatccttt aacggagcgg gcaagagcag catgttcaaa cttattgtag ccttttgcct
     2161 tttttgattg tttttgtgat agatagtcca ggtattttga cagttgctgg catgctgttt
     2221 ctgccgggtg agaaaaaggg cctaaccctt cgattaagct taatgaaaga aaacgatccg
     2281 gcgcaacccc tgctacaaga cttcccaagc aggctcccat agagtgccct agcaagtgaa
     2341 gcttatttag tcccagggca ttaataattt caacaacagt aaatattcca tcagtaaaat
     2401 gatatatgca tcctgggggt aagtgtgaag aatgaccatg cccaggcaga tctacggcta
     2461 taaaatagta atcattctgt aaatgttcag caatatcatc aaaactatta gcattatcta
     2521 accaaccgtg caaggcaaga ataggcggat tatcaggatt tccccatact ttgcacgcta
     2581 tagatagtcc cgggatactt aaaatctttg tattcatcaa ttttctttgg tttataggta
     2641 atagttatag cttattactt tatacgcagg gatgtaaatt cagtttttaa aatagtctgg
     2701 gcttgcgaaa aaaaacaatc tcttcgctaa aaaatgcttt taatccagtc atttagttta
     2761 tttaatctcc ctaattaaaa tcaatttttg ccttgatctt ggtttttttg caagtcttga
     2821 tagtaattga aatagacaaa aagcaataaa ataagtcgct tattggattg acttgtaaca
     2881 aactataaaa gatcacaaaa taaatggctt tactggtttt ttgggaaggg tc
//
LOCUS       DBNCWK010000204         2923 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.205, whole genome shotgun sequence.
ACCESSION   DBNCWK010000204 DBNCWK010000000
VERSION     DBNCWK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.205"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            4..1284
                     /gene="rvfA"
                     /locus_tag="AC06IO_002785"
     CDS             4..1284
                     /gene="rvfA"
                     /locus_tag="AC06IO_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RvfA"
                     /protein_id="HIO9875945.1"
                     /translation="MFKFIYCINQEHIMSITIHTWKGGTKGLGFKTPVKSTLFGGNVG
                     HAALELTWPANKQGDELAKKYAQAHGVIISKRTEIVGETQEEGRIMPKERVTYFAYFS
                     WWPGEQNGHYINSFNDDKDAEWRHEPDKKMHADWKNQIYGPQGMSEENTTDVKGILIK
                     NKNIQKLKTIQHSTLTADIAQDKAYNELLQHKEKLDEEYKSLHAKAVAYQKEKENAIR
                     EEREINPNFELLDEDISRMGDLPREFSILEAQLNLCIADFNERHLSSGKKPDSSVILP
                     TSMDETSLNHTLDTESILREMITLANSEKAYNFTKFNCSTSVCHIVKAGVDEKLKENL
                     SDNGFNINRYVTSYITTPTNVNALGLKLQDALLKLELAEHTKEAEQVQKPVANTISNR
                     FNEFKSRLQAVVSERNAHKDDTEEVTINNHNSLR"
     gene            complement(1469..2665)
                     /gene="mavB"
                     /locus_tag="AC06IO_002786"
     CDS             complement(1469..2665)
                     /gene="mavB"
                     /locus_tag="AC06IO_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavB"
                     /protein_id="HIO9875946.1"
                     /translation="MPPPYSTHVIVNVFRCVAQLVSQNPELLNWPGVFRLAGSREESE
                     KLLEQVINDNFSVGNLACYILEDGQINNLHLNNVLGMLPLVLKESVLLKTEDPLLKLF
                     MTELKNLLSSEKNNVENTDAIVQLLSKFIDSLLLSKILDHQRVGEILYHYCYLMHTAG
                     VFYETNLMTWENLAIIMAPHFTNEFGLYPAEDLLGLIQFTNQLKPILECFIAHPDSGI
                     PFKERHADKLEHLAKTRHTIVEKLTHMGSESRRIVVAPMKSLMLQASMLRAQIDTVET
                     QSKDSSLKKKDKKELNRQLEKLTEELEKLNIEISGLTSKIKKMNHGHAKLKEEIRVIS
                     RSEDAVSTHAAQQSGVSSSSSNSVRIQLGIFGNNSSSSAYLIEEQEEGEVTENEYSGL
                     DSAKLT"
BASE COUNT         1013 a          518 c          495 g          897 t
ORIGIN      
        1 aatatgttta aatttattta ttgcattaac caagagcata tcatgtcaat tactattcat
       61 acttggaaag gaggtactaa aggattaggc tttaaaaccc ctgtaaaatc tactttattt
      121 ggtggtaatg tgggacacgc tgctttagaa ttaacttggc cagctaataa acaaggagat
      181 gaattagcaa agaaatacgc acaagcccat ggcgtaataa ttagtaaaag aactgaaatt
      241 gtaggagaaa ctcaagaaga aggtaggatc atgccaaaag agcgagtaac ctactttgcc
      301 tatttcagct ggtggccagg agagcaaaat ggccattata ttaattcttt caacgacgat
      361 aaggacgctg aatggagaca cgagcccgat aaaaaaatgc atgctgattg gaaaaatcaa
      421 atctatggcc cacagggaat gagtgaagaa aatacaacag acgttaaggg aatactgatt
      481 aaaaataaaa acatacaaaa attaaaaact attcagcaca gtactctgac agctgatata
      541 gctcaggata aagcttataa tgagcttcta caacataaag aaaaattaga cgaagaatat
      601 aaatcattac atgcgaaagc agtcgcatat caaaaagaaa aggaaaatgc tatacgagaa
      661 gaaagagaaa taaatcctaa ttttgaactg cttgacgaag atatttctag aatgggagat
      721 cttccaagag agttctcaat tttagaagcg caattgaatc tatgcatcgc agattttaat
      781 gaaagacatc tgagttcagg aaaaaaacct gattcaagcg tgattttacc tacctctatg
      841 gatgaaacca gtttaaatca taccttagat acggaaagca ttttacgaga aatgatcact
      901 ctagcgaaca gtgaaaaagc ttacaatttt accaaattta attgctctac ctcggtttgc
      961 catattgtga aagcaggagt ggatgaaaaa ttaaaggaaa atttaagcga taatgggttt
     1021 aatataaatc gttatgtgac ttcctatatc accactccaa ccaacgtaaa tgctttagga
     1081 ttaaaattac aagatgcgct gcttaagctt gaactagcag aacatacaaa ggaagcagaa
     1141 caggtacaaa agccagtggc taatacgata agtaatcgtt ttaatgaatt taagtcaaga
     1201 ctgcaagccg tagtatcgga gcggaatgcg cataaagatg acaccgaaga agtaacgatt
     1261 aataatcata attccctgag gtagtgttta aatgtagtag ttcaaacttg atctgacagt
     1321 taccggtttt tcaagaagtg tctgtcaggt caaatttagt ttataaattt ttccatccag
     1381 atttgtttat cctctaccaa agtttgcatt ggagtacgac cacaacacat tttaccgccc
     1441 aagagatctc aattaaaaac tattgaattt aagtcagctt tgcactatct aagccactat
     1501 attcattttc agtaacttcg ccttcttcct gttcctctat caaataggca gatgaagaac
     1561 tgttgttacc aaaaattcct aactgaattc tgactgagtt actactgctt gatgatactc
     1621 ctgattgttg ggcagcatga gtacttacag catcctcaga gcgcgatatc actctaattt
     1681 cttcttttag cttcgcatgg ccatggttca tttttttaat tttgcttgtt aatccagaaa
     1741 tttctatatt cagtttttct aattcttctg ttaatttttc taactgtcta tttaattcct
     1801 ttttatcctt ctttttaaga gaggaatcct tcgattgagt ttctacagta tcaatctggg
     1861 ctctcaacat agatgcttgc agcatcaaac ttttcatagg agccacaact attcttctgg
     1921 attcacttcc catatgcgtt aatttttcaa cgatagtatg acgagtttta gccaagtgct
     1981 ctaatttatc ggcatgcctt tctttaaaag ggattccgct atcaggatgc gcaataaaac
     2041 attccaggat gggttttaac tgattagtaa attgtattaa acccagtaaa tcttctgcag
     2101 ggtataaacc aaattcatta gtaaaatgag gggccataat aatagcaaga ttttcccatg
     2161 tcatgagatt agtttcataa aaaacccctg ctgtatgcat taggtaacaa tagtggtaaa
     2221 gaatttctcc aacacgttga tgatcaagta tttttgaaag caataaagag tcaataaact
     2281 tactcagcaa ctgtactatt gcgtcggtat tttcaacatt attcttctca gaactcaata
     2341 gattttttaa ttctgtcata aaaagcttga gcaatgggtc ttcagttttc aacaagacac
     2401 tttctttaag aactaaaggc aacattccta aaacattatt cagatgtaaa ttatttatct
     2461 ggccatcttc taaaatataa cacgctaaat tccccacact aaaattgtca ttaataactt
     2521 gctcaagtaa cttttctgac tcttctctag atccagccaa tcgaaatact cctggccagt
     2581 ttaatagctc tggattttgt gacactaatt gggcaacaca tcgaaataca ttgacaatca
     2641 catgagtact atatggtggt ggcataacaa attcccagat taaattttga acaaaatatc
     2701 atgggatcaa aactaaatca attattgtta atgaaatacg aacctgaatt tttccagcca
     2761 cattaatgtc ttttggatta attgaattca tctcaagtgt tacttgcgat gaaacttgtg
     2821 ttccagaata aaatcattat tgtctaatca ggaacggcct attctattag gccgttcctg
     2881 attaatttga ggaagcaaaa atcactttaa aattttaatc att
//
LOCUS       DBNCWK010000205         2857 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.206, whole genome shotgun sequence.
ACCESSION   DBNCWK010000205 DBNCWK010000000
VERSION     DBNCWK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.206"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            160..1374
                     /gene="lbtB"
                     /locus_tag="AC06IO_002787"
     CDS             160..1374
                     /gene="lbtB"
                     /locus_tag="AC06IO_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin export MFS transporter LbtB"
                     /protein_id="HIO9875947.1"
                     /translation="MNKPVQKMMLSGQFLMILALEMTNPFLPLLIAHQSNTPVPSVVI
                     YNTLSLILPMLANILLTPIWGLAADRYGYKPMLMRASWALVLTQASMIVVNSVFWILI
                     IRLLQGAFAGFLVAMQTYALSITEWQNKSTQLSRLQSSKAIATSTAGFLGGLALSFTN
                     YQGLFGLATLICLSTTVAMHYKLPSPPKHQIKKKSQTLNHSYTSKSVFFFLCVLIMLT
                     QIAKFLPDPGFTLYLNKYCSNNLVLIGFLYSLPAMGMLCSSVWCGKQFDYCRSQPSLV
                     NQYLIGYSIFGAILMIIQANVDNFYLFALIRILWGIVLAALLPALFTLCSDRNLLPGY
                     ALGLANSFAKLGNLIGLLLGGLFAFYLPYPAIFMIIAGIYSLLAIFVYGYDRLNVRQM
                     TDFSNSYFNVKS"
     gene            1371..2531
                     /gene="lbtC"
                     /locus_tag="AC06IO_002788"
     CDS             1371..2531
                     /gene="lbtC"
                     /locus_tag="AC06IO_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038838172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin import MFS transporter LbtC"
                     /protein_id="HIO9875948.1"
                     /translation="MKKNALLIVLLTWFLGNMDIHFLTPALPSLVDYFSVTPNTAQLT
                     ISLFLLGKALSMILWGLLSERYGRKPIFIGGLLLFILSNFLIVLNHSILLFLVCRFLQ
                     GISVGATLLMGRAMINDTHNEQNATKYFAWLFTLAGLFICFLPFFGGLINSYWNWQIA
                     SLIIAAYGLLLLPLCRGLKETKPHYVKFPRLGHSIMVVFNHPLFVSYLLISALMMAGE
                     SAFNTSASFILIKGENFTSAQYGTIKTTMAIMHLLGTACCGLIAKYYHSIQLTKLGVR
                     FFIFAAFLMWLFVFSAENIYLTFIVPMVIYYFGTGFIVASATASAVRPFPQQMALALA
                     ITLFCQFNCSAFFSFITSMIGIQQVETFMFLLSLVSILSFLALLKLQRMEPC"
     gene            2675..>2857
                     /locus_tag="AC06IO_002789"
     CDS             2675..>2857
                     /locus_tag="AC06IO_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875949.1"
                     /translation="MDSNFKISIRVSIITLFVLLLSLVGFTIIGINYIAFNSVLNSSA
                     KDSIEQTSLLVKERFEI"
BASE COUNT          797 a          551 c          500 g         1009 t
ORIGIN      
        1 aaaattttaa aaaggaaatt aatccattga tataccaaaa attccaacaa catttattct
       61 ctcttccctg gcaacataaa agtttgctga gcatgagact caatcaaaat caggataacc
      121 tggtattttt tagtcttgac aacccattga gccaagataa tgaataaacc agttcaaaaa
      181 atgatgctct caggtcagtt tttgatgata ttggccttag aaatgacgaa tccctttcta
      241 ccactattga tcgcgcacca aagcaacaca ccagtaccat cggtagtgat ttataacacg
      301 ctgagtctaa ttctaccgat gctggctaac attctattaa ccccaatctg gggcttggca
      361 gctgatcgat atggctacaa acccatgtta atgagagcat cctgggcctt ggtgttgacc
      421 caggccagta tgattgttgt aaattctgta ttctggattt taatcatccg attactgcaa
      481 ggagcttttg caggttttct agttgcaatg caaacctatg ccctaagcat tactgaatgg
      541 caaaacaaaa gcacacagtt atctcgcttg caatcctcca aggcaattgc cacttctaca
      601 gccgggtttc tcggaggatt agctttaagc ttcactaact atcaaggatt atttggtttg
      661 gcaacattga tatgcctgag cactactgta gcgatgcact acaaattacc ctcgccacca
      721 aagcaccaga taaagaaaaa atcacaaaca ttaaatcatt cttacacatc aaaaagtgtt
      781 ttcttctttc tttgcgtatt aatcatgcta acccaaattg ccaagttttt acctgatcca
      841 gggtttaccc tgtatctaaa taagtattgt tcaaataatc tggtactaat tggatttctt
      901 tattctttac cggcaatggg gatgctatgt agttcagtat ggtgtggcaa acagtttgac
      961 tactgtcgaa gtcaaccttc tttggttaac caatatttaa taggctatag catattcgga
     1021 gccatcctca tgatcattca agccaatgta gataatttct atctgtttgc tcttatacgt
     1081 attttgtggg ggatcgtact tgcggcctta ttgcctgcgt tatttacttt gtgcagtgat
     1141 cgtaatttgt taccaggtta tgccttggga ttagccaatt catttgcaaa actgggaaat
     1201 ctaattggat tgttattggg tggtcttttt gcattttatc taccatatcc tgcaattttt
     1261 atgataattg ccggaattta tagtttatta gcgatttttg tttacgggta tgatcggcta
     1321 aatgtcaggc agatgacaga tttttctaat tcttatttta acgtgaaatc atgaaaaaaa
     1381 atgctcttct tatagtgtta ttaacctggt ttcttggaaa catggatata catttcctta
     1441 ctccagcctt gccatcatta gttgattact tctcggtaac ccccaataca gctcaactaa
     1501 ctatcagtct ttttcttttg ggtaaagccc tcagtatgat tttatggggt cttctttccg
     1561 aacgttatgg gagaaaacca atcttcattg gaggactgtt gttatttatt ctgagtaatt
     1621 ttctgattgt tctaaaccac tccatccttc tctttttagt atgccgattt ctccagggaa
     1681 ttagcgtagg cgctaccctt ctaatgggta gagcaatgat taacgatacc cataatgaac
     1741 aaaatgccac aaaatacttt gcgtggttat ttactctggc tgggctgttt atttgctttc
     1801 ttccattttt tggaggcttg ataaacagtt attggaattg gcaaatcgcc tctcttatca
     1861 tcgctgccta tggcctgctt ttattacctt tatgtcgagg tttaaaagaa accaaacccc
     1921 attatgtaaa attccctcgc ctaggccata gtattatggt ggtcttcaac catccccttt
     1981 ttgtttctta cctccttatt tctgccttga tgatggccgg agaatcagcg tttaatacca
     2041 gcgcctcttt tattcttatt aaaggagaaa attttactag cgctcaatat ggtacaatca
     2101 agaccactat ggcaattatg catttattag gtaccgcttg ttgtggctta atagcaaaat
     2161 attatcatag tatccaatta acaaaattag gagtacgttt ctttattttc gcagcattct
     2221 tgatgtggtt atttgtcttt tctgctgaaa atatatattt gacttttata gtgcctatgg
     2281 tgatttatta ttttggtaca ggttttattg ttgcatcagc aacagcatca gctgttcgcc
     2341 cttttcctca gcaaatggct cttgcattag ctatcacttt attttgtcaa tttaactgct
     2401 ccgctttctt tagttttatt accagcatga taggaattca gcaagtagaa acttttatgt
     2461 tcttattaag tttggtaagt attttgagtt ttcttgccct tctaaaactt caacgaatgg
     2521 agccctgctg aaaaaaccat gatctttgat cctggtaaga aaatataaag tttgcgtgta
     2581 tttctgccta aagaagacta tgcttattta aaataagttc ttaatcgtat ttcgcttaga
     2641 acttgtgtac ttccttcaat tagaaggaaa aaatatggat agtaatttta aaatcagtat
     2701 acgtgtcagc atcattacct tatttgtgct tttgctaagc ctggtagggt tcactattat
     2761 tggtattaat tatattgctt tcaatagtgt tcttaacagt agcgctaaag attcaattga
     2821 acaaacatca ttactcgtca aagagaggtt tgagatc
//
LOCUS       DBNCWK010000206         2746 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.207, whole genome shotgun sequence.
ACCESSION   DBNCWK010000206 DBNCWK010000000
VERSION     DBNCWK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.207"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1065
                     /locus_tag="AC06IO_002790"
     CDS             <1..1065
                     /locus_tag="AC06IO_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIO9875950.1"
                     /translation="ISLCFFSLILWFGSPILLAQPSRLDNLINQLVQPFIYAHQTPGL
                     AIAIYYNGKDYYYNFGFADRKSKKPVTKNTIFELASITKIFISTLIALEVQKGKLNVT
                     DYAVQYTPELSKTNGLPIDRVQIVHLATHTASFPKQMEQFGVNKGNIQAFFARLKTWR
                     PPVKTGTHYKYSNIGFGYLGYVLENASNETLPNLLKSNITKPLEMSHTFFNVPESLAN
                     YEAQGYRPKNKPAPYYRPANFLGGGALRSSAADMLVFLKANLGIQPGTASPELLSAMQ
                     YAQQPFFEVNPNFVMGLGWQRVKRGRQIVITKNGANQGFSTFIGFSPAKKLGVVVLIN
                     QARGKATLLGNQILNSLLRL"
     gene            1260..1697
                     /locus_tag="AC06IO_002791"
     CDS             1260..1697
                     /locus_tag="AC06IO_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875951.1"
                     /translation="MKKSSPVYSLLLLSAITFSPVIKASPIGMMVDIYCPITQGGPNV
                     ITNFGDYIGGYGMENILSQNNPIYFKSISLAHDVPAQLGNYYNETTSYNSTTGQVTCS
                     YISNNPTEPRFAVAYTLTNGRGGSVQWQSGNSINIIFPVGLKS"
     gene            1718..1885
                     /locus_tag="AC06IO_002792"
     CDS             1718..1885
                     /locus_tag="AC06IO_002792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875952.1"
                     /translation="MPDCLLRLPATLSGIDLLGPYNQSQSQTKQKNIPDNWDIQAETH
                     DLYDFNSGQIS"
     gene            complement(1903..2196)
                     /locus_tag="AC06IO_002793"
     CDS             complement(1903..2196)
                     /locus_tag="AC06IO_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875953.1"
                     /translation="MNVNSENSQSGEAVTAEDYSRAMNFIGQNLLSSLTQSVEKLPPQ
                     FRNRRLVCQALSAFLTNVIYKQFPEQPESCQQMLDDITRHVSMQLDKIPQPSK"
     gene            complement(2345..2536)
                     /locus_tag="AC06IO_002794"
     CDS             complement(2345..2536)
                     /locus_tag="AC06IO_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HIO9875954.1"
                     /translation="MLYWALIFLIVAIVAGLFGFRGVASAATGIAKVLFFLFIVMFIV
                     LLVFSILGGTPEPVVIVKP"
BASE COUNT          905 a          547 c          479 g          815 t
ORIGIN      
        1 attagcttat gctttttttc actcatttta tggtttggca gtccaattct tttggcacaa
       61 ccatccaggc tggataatct aattaatcaa ctggttcaac catttattta tgcacatcag
      121 actcccgggt tagccattgc catttattat aatggaaagg attattatta taattttgga
      181 tttgctgacc ggaaaagtaa gaagccagtg actaaaaata ctatttttga attggcatcg
      241 attaccaaaa tattcattag cactcttata gcactggaag ttcagaaagg aaaattgaat
      301 gtaacagatt atgccgttca atatacacct gaactttcaa aaaccaatgg actaccaatt
      361 gacagggttc aaattgtgca tctggcgact cacacagcaa gctttcccaa acaaatggag
      421 caatttggtg ttaataaagg caatatacag gctttctttg cccgtcttaa aacatggcgt
      481 cctccagtaa aaacaggaac tcattataaa tattccaata ttggttttgg gtatttggga
      541 tacgtattgg aaaacgcctc aaacgagact ttacctaatc tgttaaaatc gaatattacc
      601 aagccattgg aaatgtccca tacttttttt aatgtaccag aaagcctcgc taactatgaa
      661 gctcaaggtt atcgacctaa aaataagcca gcaccttatt atagacctgc taattttcta
      721 ggcggaggtg ctttgcgatc ttctgcagct gacatgctgg tatttttaaa agcaaattta
      781 ggaattcaac ctggcaccgc atcaccagaa ttgctttctg cgatgcagta tgcccaacaa
      841 ccattttttg aagtcaaccc aaattttgtt atgggattag gttggcaaag agtgaaaaga
      901 gggaggcaga ttgtgatcac taaaaatgga gcaaatcagg gatttagcac ctttattggt
      961 ttttcacctg caaaaaaact gggagtcgtc gtattaatca atcaggcaag gggcaaggca
     1021 accttgctgg gaaaccaaat tcttaatagt ctgcttagat tatagcttaa aggactattt
     1081 ttaagcatag actcatgggc attgacaatc aaaatgtcgt gcaacatgat tatccttgac
     1141 cctattctct gtgcgtcttg acttacaata tggatagtat gattttaatc taaactaatg
     1201 acgaacagag cctcaggtca aaccaacaac ttatcaaata actgaaaaag gaagtttcaa
     1261 tgaaaaaatc cagtcctgta tactcgttgt tattgctttc tgctattacc ttttcgcctg
     1321 ttatcaaggc aagtcctatc ggtatgatgg tggatatcta ttgccctatt acccagggag
     1381 ggccaaatgt tatcactaat tttggagatt acattggtgg ttatggcatg gaaaatattt
     1441 tgtctcaaaa taatcctatt tattttaaat ccatttcgct agcccatgat gttccagccc
     1501 aacttggaaa ttattacaat gaaacgacca gctacaacag cacaacaggt caagtcacct
     1561 gcagttacat aagcaataac ccaacagaac ctcgctttgc tgtggcgtat accttaacca
     1621 atggcagggg cggttctgtc caatggcagt cagggaattc aatcaatatc atttttccag
     1681 ttggtctaaa aagttaacca aagtttactg gattaaaatg cctgactgtc ttcttcgcct
     1741 cccagcaact ctttctggaa ttgatttgct gggaccctac aatcaaagcc aaagccaaac
     1801 caagcagaag aacatacctg ataattggga tattcaagct gaaacgcatg atttatatga
     1861 tttcaactcg ggccaaatta gctgatacag attactagcg ttttactttg atggttgagg
     1921 gattttatcc agttgcatac tgacatgcct ggtaatatca tcaagcattt gttggcatga
     1981 ctcaggttgc tctggaaact gtttataaat aacattagtc aaaaatgcag acaaagcctg
     2041 acaaactaac ctgcgattac gaaactgtgg cggcaacttt tccacagatt gagttaatga
     2101 tgacaataaa ttttgaccta taaaattcat tgccctggaa taatcttcag cggttacagc
     2161 ttcgcctgat tgactgtttt cactattcac attcattttt tatcctgtca gtgtgtgatc
     2221 aagccagatt atagagtcta tctcaagcta cttcaacgac tgtttcaact ctttctgtaa
     2281 aagcgacatg attatcaacc aagacaacaa aatacattat taacaggact tatatagaat
     2341 gtagctatgg ttttacaatg acaacaggct ctggtgtgcc gcctaaaata ctaaagacca
     2401 aaagcacaat aaacattacg ataaagagga aaaacaaaac tttggctatg cctgttgcgg
     2461 ctgatgcgac gcccctaaat ccaaataaac ctgcgacaat tgcaacaata agaaaaatta
     2521 aggcccaata taacataata aactccctgt tttcagaata atcctttctt cctgaattgt
     2581 ttttaccgaa gtaaaatcaa gccacccctg cttatgatca tctcacttat aaaaaaacaa
     2641 actgaaatac ttatttatag tcatcatatt aaaattgtag actatctatt caaaataaag
     2701 aaaaacaaac aataacgcat aacagcatgc aagaaataaa ctgttt
//
LOCUS       DBNCWK010000207         2741 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.208, whole genome shotgun sequence.
ACCESSION   DBNCWK010000207 DBNCWK010000000
VERSION     DBNCWK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.208"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            113..583
                     /locus_tag="AC06IO_002795"
     CDS             113..583
                     /locus_tag="AC06IO_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIO9875955.1"
                     /translation="MKRLIPTFIYLLVLAITPFSTHAEALLKDTFGHNIPFSSLKGKW
                     VFINYWASWCETCVDEIPEFNRFYKNHKNEAVALYAVNYDALPLPEQKLLIKKFNINY
                     PNLLKDPAQDLQLGDITGVPVTFVFNPKGQLIKKLYGGQTAKTLEKVIAENRTS"
     gene            complement(688..1932)
                     /gene="hflX"
                     /locus_tag="AC06IO_002796"
     CDS             complement(688..1932)
                     /gene="hflX"
                     /locus_tag="AC06IO_002796"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HIO9875956.1"
                     /translation="MFERPQGGERAVLVQLALPGVDADKALAEFEELALSAKAEVLDC
                     VLGARATPDAKYYVGKGKAEEIAHLVNMLDADLVLVNHELSPSQERNLERLFGCRVVD
                     RSGLILDIFAQRARTFEGKLQVELAQLQHLSTRLIRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRERIRYINKRLEKVRCSRDQNRQARRKASMPTVSLVGYTNAGKSTLFNVLT
                     GEHTYVADQLFATLDPTMRKLELPGSSAAILADTVGFIRDLPHHLVEAFRATLEETQQ
                     ADLLLHVIDISDPNWRETVFEVQKVLDDLKVNNIPVIQVFNKIDLQEGWQPKIDYTED
                     SCKVWLSATTGAGLDLLKEAIATQLHGMILIEDIVIKPDQAKLRAQLYQLGAVLKESV
                     DENGDWLIKIRITADQKRRLLS"
     gene            complement(1960..2217)
                     /gene="hfq"
                     /locus_tag="AC06IO_002797"
     CDS             complement(1960..2217)
                     /gene="hfq"
                     /locus_tag="AC06IO_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIO9875957.1"
                     /translation="MSKNHLLQDPFLNELRKEKVPVSVFLVNGIKLHGIIDSFDQYVV
                     MLKNSITQMVYKHAISTVVPSRMVKIPAEESSGEEEGTVAD"
     gene            2405..>2741
                     /locus_tag="AC06IO_002798"
     CDS             2405..>2741
                     /locus_tag="AC06IO_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0008 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875958.1"
                     /translation="MTFTCDELKGLEHPYEVLGNGDALAENREELNKLTNDEKLVLAS
                     RLVLECPVNELKDFAHAIEAARMPQDDSDTFHSFLFQAYQVKKRIISLLDPRNKNPHS
                     MILEKEFDGE"
BASE COUNT          845 a          597 c          480 g          819 t
ORIGIN      
        1 tgatctgtcc taaagttagt aaattggaca taagcaaata catgcatttt cttatgtcca
       61 atttggctaa caataataac cgtaaacaat tttgactatt caagaggtga tcatgaagcg
      121 cttaatacct actttcatat acctgctggt tttagcaatt actcccttct ctactcatgc
      181 agaggcgctt ttgaaagaca cctttggcca caatattcct ttttcatcgc ttaaaggaaa
      241 atgggttttt atcaactatt gggcaagctg gtgtgaaacc tgtgtcgatg aaatccctga
      301 gtttaatcgc ttttacaaaa accataaaaa tgaggcagta gccctatacg cagtcaatta
      361 cgatgctctt cccttaccag agcaaaaatt actaattaaa aaatttaata ttaactaccc
      421 taatttgtta aaggatccgg ctcaggattt acaattagga gacatcacag gagtacctgt
      481 aacttttgta tttaacccaa aagggcaatt aattaaaaaa ttatacgggg gacaaacggc
      541 aaaaacatta gaaaaagtca ttgcagagaa caggacctcc taagaagata aataaaaatt
      601 atatttttgg ttaataagtt aaagattttc ttgtgaattt atcaagtctg gaatcgattc
      661 tatcgccccg cagcctgact tgattattca tgaaagcaat cgtcgttttt gatcagccgt
      721 tattctaatt tttattaacc aatcgccgtt ttcatctaca gactctttaa gtacagcgcc
      781 taattggtat aattgggctc gtaattttgc ctggtcaggc ttaattacaa tatcctcaat
      841 taaaatcata ccatgcaatt gagttgctat tgcctctttt agaagatcca gaccagcgcc
      901 ggttgttgct gataaccata ccttgcaaga atcctcagta taatcaattt ttggttgcca
      961 accttcctgc aaatcaattt tattaaatac ctgaattacc gggatattat taacttttaa
     1021 gtcatctaac accttttgta cttcaaaaac cgtctctcgc caattcgggt cagaaatatc
     1081 aatgacatgc agtaatagat cagcttgctg ggtttcctct aaagtagcgc ggaatgcttc
     1141 taccaagtga tggggaagat ctcgaataaa tcctactgta tctgctaaaa ttgctgctga
     1201 ggaccctggc aattccaatt tacgcatcgt cggatccaag gttgcaaaca attgatcagc
     1261 cacataagtg tgttcccctg ttaaaacatt aaataaagtc gattttccag cattggtgta
     1321 acccactaga gaaacagtag gcatggacgc cttccgcctg gcctgcctgt tttgatcgcg
     1381 gctgcaacgt actttttcca atcgcttatt gatataacga atgcgctctc ttaacaaacg
     1441 ccggtccgtt tccaattggg tttctcctgg gcctcttaaa ccaatccctc ctttttgcct
     1501 ttccaaatgg gtccatccac gaataagcct tgttgataaa tgttgcaatt gggctaactc
     1561 aacttgaagt ttaccctcaa aagtacgagc tctctgagca aaaatatcaa gaatcaagcc
     1621 acttctatcg acaactcgac atccaaataa tcgctcgaga ttccgctctt gagaaggcga
     1681 tagctcatga ttgactaaca ctaaatccgc atcgagcatg tttaccaaat gagctatttc
     1741 ctccgcctta cctttcccta cataatattt ggcatcggga gtagctcggg cacctaatac
     1801 acaatccaac acctcagcct tcgccgaaag cgctaactct tcaaactcag ccaatgcctt
     1861 atcagcatca accccgggga gagctaactg caccaaaaca gctcgctcac cgccttgtgg
     1921 acgttcaaac actaccctaa tccccaaaca agaaattgat tagtctgcca cagttccttc
     1981 ttcctcacca gagctttcct cagcaggaat tttgaccatt cgagaaggaa caactgttga
     2041 aatggcgtgt ttataaacca tttgggttat ggagttttta agcatcacca catattgatc
     2101 aaaagaatcg attatcccat gcaatttaat accattgacc aggaacactg aaacaggtac
     2161 cttttccttg cgcaattcat ttaggaaagg gtcttgtagt aaatgatttt tagacattgc
     2221 ctactccttg ttgttattgt aatttttaaa ggcaaaaacg ggtatctaac tatttattcg
     2281 acaccctata ataaaacttt agtatatttt catgacagcg tggtttgaat tcgttaatat
     2341 agtcaatcaa aatatttttg cacatctaga atcaggctac tgatgagtta ataaggagat
     2401 tttaatgacg tttacttgcg atgaattaaa aggattagag cacccctacg aagtacttgg
     2461 caatggtgac gcactggcgg aaaaccgaga agagctaaac aaattaacca atgatgaaaa
     2521 attagtttta gccagccgcc tcgttttgga gtgtcccgtt aatgaattaa aagattttgc
     2581 tcatgccatt gaagcagcaa gaatgccaca agatgatagc gatacctttc attccttttt
     2641 attccaggct tatcaggtaa aaaaaagaat catttccttg ctcgatccgc gcaataaaaa
     2701 ccctcattcc atgatattgg agaaggaatt tgatggtgag c
//
LOCUS       DBNCWK010000208         2723 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.209, whole genome shotgun sequence.
ACCESSION   DBNCWK010000208 DBNCWK010000000
VERSION     DBNCWK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.209"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            264..2591
                     /locus_tag="AC06IO_002799"
     CDS             264..2591
                     /locus_tag="AC06IO_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidE phosphodiesterase domain-containing
                     protein"
                     /protein_id="HIO9875959.1"
                     /translation="MKTKLNKSILFKQGNAGELFESFIQKVDENAKPNQLEEIIAVAK
                     PQVIAPVTKNTYTKQFEIIVSTLLTNKSNIVFTEKNGKYFVNIKGTETNLDDILVNMQ
                     RSQINLSTEQLNQYVNYVDTEATTKIELTNGKMSCKELKKKATKLQHLHEGEIAGLNI
                     YTQQYYQFMNGLLRGQHPYKGSDANFQENMRQVILHSAAALSGISKGQNHKLPLTFRY
                     DGNSLPKAIIEKRIQCAQSKGIELEVYEDTAFVSSAFEKPVSTFNGPIRTVFYGMQGL
                     DIGEISRYPAEREFLIAPGKVKYLHHREENGKHYFLACPVTPPASIKQILNLPNGGTY
                     KQAENITLEAEKDMVKKFAQFALDVTNQYLEGKAEKDKSKWSILANIDNTYGKGSYKI
                     QFAEEIKKSMNGLLANLKNGYVEHKAAHSRIIEILHQAIEKAQTIDNISLSYAKGLDS
                     AGVLSKTLQNICFQVEDEMKGLEVFKSSITEYFADHQSFFENPRNIEVINKLHQDHLS
                     KPYDPSEKNKDPNSGDWTLNCGNNKILHRPNHGLSHTLRGAALVPEVLYSYATHSTDK
                     KTRDMVQNISKDDIKRMQVAMLFSVVGRKSELGFADNPGVYGGYRKASAELFEQYAKT
                     HYKDLFTSPEQIAYWKRLVLDYGNPGFHPDVNKPEQAIIAKIMRQCHCLDLLRCYSKE
                     EFAKKVTKPLQDDLGIDAANSLVNYSKTLLEKTGDRLIGTKSYNLQEHWLNNTDVSHC
                     ISILNSASQHAPHQQYVVKHEEEETLDSDMWVTLN"
BASE COUNT         1032 a          501 c          457 g          733 t
ORIGIN      
        1 tcttaggaca ctttatcaca agatatttac ctctcccaaa caaaatttta tccccttgat
       61 aacccaaaac atatttagtt cttatctttc ttaatattca aaatttgaac taatagatta
      121 gaaaacaaat gtcctgaaaa tttaaataca caaatgtcgc ataagcgaca tgtcgcctca
      181 tttttagata accacaaaag gttaatattt aatcataaag attaattata tatctttaag
      241 attaaatatt aacgaggtgt atcatgaaaa ccaaactaaa caaatcaatc ctttttaaac
      301 aaggaaatgc tggtgagctt tttgaatctt ttattcaaaa agttgatgaa aatgccaagc
      361 ctaatcagct tgaagaaatt atcgctgttg caaaacccca agtcatagcc cctgtaacaa
      421 aaaatacata taccaaacaa tttgaaatta tagtatccac tttactaacc aataaatcca
      481 atattgtctt tacagaaaag aatggcaaat attttgtaaa tataaaaggg acagaaacaa
      541 atctggatga cattctggtt aacatgcaac gctctcaaat aaatctgtct actgaacaac
      601 taaatcagta tgttaattac gttgatacag aagcaacaac taaaattgaa cttacgaatg
      661 gaaaaatgag ctgcaaggag ctaaaaaaga aagcaacgaa attacaacat cttcatgaag
      721 gtgaaattgc tggattaaat atatataccc aacagtatta tcaatttatg aatggtttat
      781 taagaggcca acatccatac aaaggaagcg atgcgaactt tcaggaaaat atgcgtcagg
      841 taatacttca tagcgcagca gcactctcag gtattagcaa aggacaaaac cataaacttc
      901 cattgacctt ccgatacgac ggaaacagcc ttcccaaagc tattattgaa aaaagaattc
      961 aatgtgccca gtcaaaaggt atagaactag aagtttatga agacaccgct tttgtcagta
     1021 gtgcttttga aaaacccgta tctactttta atggacccat tcgaaccgta ttttatggta
     1081 tgcaaggatt ggatatagga gaaatctctc gttatcctgc tgagcgtgaa ttcttaatcg
     1141 ctcctggtaa agtcaaatac ctgcatcatc gagaagaaaa tggaaaacac tatttcctgg
     1201 cgtgtccagt cactcctcct gcaagcataa agcaaatatt aaatttgcca aacggtggta
     1261 cttataaaca agctgaaaac ataacactag aagcagaaaa ggatatggta aaaaaattcg
     1321 cacaatttgc tctcgatgtt acgaatcaat atcttgaggg taaagcagaa aaagataaaa
     1381 gcaaatggtc aattttagca aatattgata atacctatgg aaaaggctct tataaaattc
     1441 agtttgcaga agaaataaaa aaatccatga atggattgct tgccaattta aaaaatggat
     1501 atgttgaaca taaagcggct cactccagaa ttattgaaat acttcatcag gcaatcgaaa
     1561 aagcccaaac catagataac atctccttat cttatgctaa aggattagat agtgctggtg
     1621 tgctatctaa aaccttacaa aacatctgtt ttcaggtcga ggatgaaatg aagggattag
     1681 aagtgttcaa aagttcaata actgagtatt ttgcagatca tcaaagcttt tttgaaaacc
     1741 caaggaatat tgaggtaatt aataagttgc atcaagatca tctatcaaaa ccatacgacc
     1801 catcagaaaa aaacaaagac cctaatagtg gcgattggac tttaaattgc ggtaataata
     1861 aaattcttca tagaccaaat catgggctgt ctcacacttt aagaggtgct gctctagttc
     1921 cagaagtact ttattcctac gcaacgcatt ccaccgataa gaagaccaga gatatggttc
     1981 aaaacataag caaggatgac atcaaacgaa tgcaagtcgc catgttattt tctgttgtcg
     2041 gcagaaaaag cgaactggga tttgcagata accctggggt atatggggga tatagaaaag
     2101 ccagcgcaga actttttgaa caatatgcaa aaacgcacta taaagactta tttacctcac
     2161 cagaacaaat tgcctattgg aaacgcttgg tgctcgatta tggaaaccct ggttttcatc
     2221 ccgatgtgaa caaaccagaa caagcaatca tagctaaaat aatgagacaa tgtcattgtc
     2281 tggatttgtt aagatgctat tctaaagagg aattcgcgaa aaaggtaacc aaaccacttc
     2341 aagatgactt ggggattgac gcagctaaca gtctggtaaa ctactccaaa acccttcttg
     2401 aaaaaacagg agatagatta atcggtacga agagctataa tttacaagaa cattggctta
     2461 acaacactga cgtatcacat tgcatcagca tactgaactc agcttcacaa catgcaccac
     2521 accaacaata tgttgttaag catgaagaag aggaaacctt ggattcagat atgtgggtta
     2581 cactaaacta acatcaaata gagagaacct ctggtttcga gtaaaacggg gatcagaagt
     2641 tctctagctt tctttgatta ctttgtttat gcattgaatc tacaaaaata aaaaccaatc
     2701 attacaccac tcattacacc act
//
LOCUS       DBNCWK010000209         2717 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.210, whole genome shotgun sequence.
ACCESSION   DBNCWK010000209 DBNCWK010000000
VERSION     DBNCWK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.210"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            2..1684
                     /gene="legLC8"
                     /locus_tag="AC06IO_002800"
     CDS             2..1684
                     /gene="legLC8"
                     /locus_tag="AC06IO_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegLC8"
                     /protein_id="HIO9875960.1"
                     /translation="MFIFGDNMPLTSLKKFLKDIGKDKIKQHQVKLVLKTSQDIQLFL
                     NALRDSRNHGISSLSELDLSNTRFTNQELSDLVTALNNIPGIKSLRLDSCGLKDSDTV
                     ELFKLTHIKKLSLKSNYLKNRPMFNSMLEALYLDYNTELSASYVLFSLSRNAAALKKL
                     SLRNCGVTDANLEYLTRPESRLKNLTHFNLRRNNITHQGVDSIAHLQSLTTIDLSQNT
                     GIGDEGVSRLAPLKQLRTLYLDNCGIGAEGIKAIAKMNLQTVDLSFNPGLKKEWGLDD
                     IRPNHTIRTLLLTFCSLNDNHAKLIVSKFPAATDLNVANNNMTRAGVKTLLSNPIIEN
                     LDVSTQSLYAKATGASGTGMISPRIMMKKKEIKQQEKEKAQDLLDTICNTITLKSINL
                     EHTGITSRMLLSLIPDETDHKRYLKKINGVSCKELKPKLEQQIALRKAEKQSSPPVII
                     AVEDSKPSDLMIKSGSSSMTEAQTREDGLIASPDEKIKALELENQKLKDELATARKRI
                     FELENQSLSGSKTRKKTSIPPLAIPVMFQDFAKPGKNETDDNISNSITAIPK"
     gene            complement(1748..2713)
                     /locus_tag="AC06IO_002801"
     CDS             complement(1748..2713)
                     /locus_tag="AC06IO_002801"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9875961.1"
                     /translation="MDLSKRIEPKTWSFVEKIQNAGGKPIYDIPVAEGRAIFDQIQAL
                     PNEKPDVDVEDKTLPVGPGGKVDIRIVRPKGAKEALPVIMFFHGAGWVFGDYQTHGRL
                     VREIAVGTHAAVVFVKYTLAPEAQYPTQIEESYAAMKYIAEHGKQFNLDTSRFVAAGD
                     SVGGNMTSVMTLLAKERGGPQIDYQVLIYPVTDANFDNASYKEFAEGPWLTKKAMEWF
                     WDKYLPNKEKRKEITASPLQASLEQLKGLPPALVITGECDVLRDEGEAYAHKLNAAGV
                     TVTAVRHLGTIHDFLMLNDLSETPACRNAVETIIGHLTQIFGKKK"
BASE COUNT          880 a          505 c          528 g          804 t
ORIGIN      
        1 tttgtttata tttggagaca atatgcccct tacttcgtta aaaaaatttt taaaagatat
       61 tggaaaggac aagataaaac agcatcaggt aaaacttgtt ttaaaaacat ctcaggatat
      121 tcaattattt ttaaatgctt tgcgagattc gagaaaccac ggaatctcgt ccttatctga
      181 acttgatcta agcaatactc gctttactaa tcaagagtta agtgatttag tcacggccct
      241 taataatata cctggaatta aatctttacg tttggattct tgtggtctta aggatagtga
      301 cacagtggag cttttcaaat taacgcacat caaaaaatta tcattgaaaa gtaattactt
      361 aaagaaccgt cctatgttta attctatgct tgaagcgtta tatttggatt ataatacaga
      421 acttagcgca agttatgtat tatttagttt aagtcgcaat gctgctgctt taaaaaaatt
      481 atccttgcgt aattgtggtg ttactgatgc taatttagag tatttgactc ggcctgagtc
      541 gaggttaaaa aatctgacac actttaattt gcgaagaaat aatattactc accaaggagt
      601 agatagcatt gctcatcttc aatcgttgac tactattgat ttaagtcaaa atactggaat
      661 tggtgatgag ggtgtatcca ggcttgcgcc tttaaagcag ttaagaaccc tgtatttgga
      721 taattgtgga ataggggcag aaggaattaa ggcaattgcc aaaatgaatt tgcagactgt
      781 tgatttgagt ttcaatcccg ggttaaaaaa agaatgggga ttggatgaca ttcgacctaa
      841 tcataccatt cgaacattat tgttaacatt ttgcagctta aatgataatc atgccaagct
      901 tattgtttcg aagtttccag cggctaccga tttaaatgtt gcgaataata atatgaccag
      961 ggcaggggta aagactttat tgagtaaccc gattatagaa aatttggatg tcagtaccca
     1021 atcgctttat gcaaaagcaa caggagcttc tggcactggt atgatctctc cccgaataat
     1081 gatgaagaaa aaggaaataa aacagcagga aaaagaaaaa gcgcaagatt tgctagatac
     1141 tatttgtaat acaatcacac taaagagtat taatctggaa catacaggca taacctccag
     1201 gatgttattg agtttaattc cagatgaaac agatcacaaa cggtatctta agaaaattaa
     1261 tggtgtttcc tgtaaagagc ttaagccaaa attagaacaa caaattgcct tgagaaaagc
     1321 tgaaaaacaa agttctcctc ctgtaatcat tgcagtggaa gattccaaac caagtgattt
     1381 aatgattaaa tctggcagta gttccatgac tgaggcgcaa accagagaag atggcttgat
     1441 agccagtcct gatgaaaaaa taaaagctct ggaactagag aatcaaaaat taaaggatga
     1501 attggcaaca gctcgtaaaa gaatttttga attggaaaat cagtctttat ctggatccaa
     1561 aacaagaaaa aaaacctcaa taccaccatt ggcaatcccg gtgatgtttc aggattttgc
     1621 aaaaccagga aaaaatgaaa cagatgacaa catcagcaac tcaataacgg caatcccgaa
     1681 atagcacaat tattgtaaat gcattcagca tgactaacat ccacttggat gttagtcatg
     1741 tattgtttta ctttttcttg ccaaaaatct gagttaagtg accaataatg gtttcaacgg
     1801 catttcggca tgccggtgtt tcagagagat cattgagcat caggaaatca tggattgtgc
     1861 ccagatgacg aactgcagtc actgtcacac ccgctgcgtt gagtttatgg gcataggctt
     1921 cgccctcatc acgcaaaaca tcacactcac cagtgatgac cagtgctggt ggtaggcctt
     1981 taagttgttc aagagaggct tgcagcggac ttgctgtaat ttcctttctt ttttctttat
     2041 tgggcaaata cttatcccaa aaccactcca tcgccttttt ggttagccag gggccttcag
     2101 caaattcttt ataggaagcg ttatcaaaat tggcatcagt tacaggataa atgagtactt
     2161 gataatcaat ttgggggcca ccacgttctt tagcaagaag agtcatgacg ctggtcatat
     2221 taccaccaac actgtcgcct gcagcgacaa atcgtgaagt atcaagatta aattgcttgc
     2281 catgttctgc tatatatttc atggcagcgt aagattcttc tatttgtgtc gggtactgag
     2341 cttccggagc taaagtgtat ttaacaaaca cgactgcagc atgagtaccc actgctattt
     2401 ctcgtacgag acgaccatga gtttgatagt cgccaaaaac ccagccagca ccatgaaaaa
     2461 acataatgac aggtaaggct tcttttgcac ctttagggcg aacaatgcga atgtcaacct
     2521 tgccgccagg ccctactggc aaagttttat cttcaacatc gacatcaggt ttctcattag
     2581 gcaaagcttg tatttgatca aatattgccc tgccttctgc aaccggtatg tcataaatcg
     2641 gttttccacc cgcattttgt attttttcaa caaatgacca ggtttttggt tctattcttt
     2701 tgcttaaatc cattttg
//
LOCUS       DBNCWK010000210         2701 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.211, whole genome shotgun sequence.
ACCESSION   DBNCWK010000210 DBNCWK010000000
VERSION     DBNCWK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.211"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            65..334
                     /locus_tag="AC06IO_002802"
     CDS             65..334
                     /locus_tag="AC06IO_002802"
                     /inference="COORDINATES: protein motif:HMM:NF017757.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIO9875962.1"
                     /translation="MPPKIQARMIKLLELIETHGANLGPPHTEAMGDGLFEIRAKAQE
                     GIGRSLYCYMKGKHIVVLHAFVKKSAKTPKPDLQLALKRKREVEK"
     gene            331..615
                     /locus_tag="AC06IO_002803"
     CDS             331..615
                     /locus_tag="AC06IO_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9875963.1"
                     /translation="MMKLKTLKAKALKNPEVKEAYDALESEFALIDALLTMRQKAGLT
                     QEELAERMGTKKGNISRLEKGNTNPSWNTLEKYAHACGFDISMAVKRIAS"
     gene            809..1630
                     /locus_tag="AC06IO_002804"
     CDS             809..1630
                     /locus_tag="AC06IO_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875964.1"
                     /translation="MISLSFLFANAHELAALLELPYVQRLVYLMGIRPYMDRQSCIVG
                     IKRRISYQSLRETLYVGPIAGVKTGSPSLQQMKRAVKSLERAGLIEIQSTERHLIVKC
                     IFAELDNPAQKKAVPKPYLYPDTNPTQEDALESGESCNFRQKPNIANNTEADPPQKSE
                     DYNYIYMCSKFEKFWKSYPHPVDREAAFYQFRELNPDDKLFEKIMARLHGQIESYNLQ
                     VMLSEWVPKWKYPANWLAKKCWQTDSFVFEQSLPVVHDLENQQIDIQEDCSLNNT"
     gene            1884..2237
                     /locus_tag="AC06IO_002805"
     CDS             1884..2237
                     /locus_tag="AC06IO_002805"
                     /inference="COORDINATES: protein motif:HMM:NF022172.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HIO9875965.1"
                     /translation="MKRDWDLVRDILLRVEDLSSNQNLTLSHFSDKDLYLVSYHVKML
                     CEAGLINANIVNDLSTQPSQFFVSGLTLSGHEFLDAIRVESIWTKTKAYFKTNGGVMI
                     FETIKEVALNMLSNH"
BASE COUNT          888 a          453 c          542 g          818 t
ORIGIN      
        1 ttaataataa atgtcttgga ttgttgaatt ttataatgag tctgttgaag aggccatact
       61 tacaatgccg ccaaaaattc aggctcgaat gattaaactt ttggaattga ttgaaacaca
      121 tggcgctaat cttgggcctc ctcatacaga agcgatgggt gatgggttat ttgagattcg
      181 cgcaaaagct caagaaggaa tcgggcgatc gctttattgc tatatgaagg gaaagcatat
      241 tgtggttttg catgcatttg taaagaaaag tgcaaaaact ccaaaaccag atttgcaatt
      301 agctctcaaa agaaagcggg aggtagaaaa atgatgaaac ttaaaacatt aaaggctaaa
      361 gcgttaaaaa atcctgaagt aaaagaagca tacgatgctt tggaatccga atttgcattg
      421 atcgatgcgt tactgacgat gcgtcaaaaa gctggattaa ctcaagaaga gttagctgag
      481 cgaatgggga caaaaaaagg gaatatttct cgactggaaa aaggaaatac taatccaagc
      541 tggaatactt tagagaaata tgcacatgcg tgtgggtttg atatttcaat ggcagtaaag
      601 agaatcgctt cataattttt gattcatatt cataacattt ctcaaaattg aataaattcc
      661 aaatattcta gtatggctag attatttgga atatttataa actcaaatta ttttttggtt
      721 caaacaatat catttagtaa caccatttct aatcatattg actatccgga tgacacatct
      781 aaaatgagaa taccgcatga ttttaaggat gattagcttg agttttttat tcgccaatgc
      841 ccatgaatta gcggcgttac ttgaattgcc ttatgtacaa agattagttt acctgatggg
      901 catccgtcca tacatggaca gacaaagttg cattgtaggc ataaagcgca ggatcagtta
      961 tcaatcgtta cgtgaaaccc tctatgttgg gccgattgca ggcgttaaaa cgggcagtcc
     1021 aagccttcag caaatgaagc gggctgttaa atctcttgaa cgtgcaggtc ttattgagat
     1081 tcagtccact gaaaggcact tgatagtaaa gtgtattttc gccgaattag ataaccctgc
     1141 tcaaaagaag gccgtaccga agccgtacct ttatcccgac acaaacccga cacaggaaga
     1201 tgctttagaa tcgggggaat catgtaattt tagacaaaaa cccaacatag ccaataacac
     1261 tgaggccgac ccacctcaga agtcagaaga ttataattat atttatatgt gtagcaaatt
     1321 tgaaaaattt tggaaaagtt atccacatcc cgttgatagg gaagcggctt tttatcaatt
     1381 cagagaactt aatcctgatg ataagttatt tgaaaagatt atggcgcgat tacatgggca
     1441 aattgaaagt tataaccttc aggtcatgtt aagtgaatgg gtgccgaaat ggaaataccc
     1501 ggccaattgg ttagccaaaa aatgttggca aacagattcg ttcgtgtttg agcaatcatt
     1561 accagttgta catgacttgg aaaatcaaca gatagacatt caagaagact gcagtctaaa
     1621 taatacttaa tttcagatca gtgaagctcg ataagtaatg ctttgtactt tgagaaagaa
     1681 gaatagcatt tctataattt attgttttct gcgtcactgc aatttcccag cagtgacgca
     1741 gaaaaattac tttaatattc cccagtaaaa tcaaggtatt ccgctatttt gtccgatccc
     1801 gcctatcggg attggacata tttttattaa cacgatggct atactgtatt taattcgcta
     1861 cttattatga aggaattttt taaatgaaaa gggattggga tttagtcagg gatattttat
     1921 taagagtaga ggatctatct tcaaatcaaa atctgacatt aagtcatttt tcagataaag
     1981 atctctatct tgtttcctat catgtaaaaa tgttatgcga ggcaggtttg ataaatgcaa
     2041 atattgtaaa tgatttaagc acgcagcctt cacaattttt tgtttctgga ttgactttgt
     2101 ctgggcatga gtttttagat gctattcgag tagagtcaat ttggactaaa accaaagctt
     2161 attttaaaac aaatggtgga gtaatgattt tcgagacaat taaagaggta gcattaaata
     2221 tgctcagtaa tcattaacta atggttcagt ttatatctgt gtcactgcaa ttttggtgca
     2281 gtgacgcaga tatatgcttc ccatattatc cagtaaaatc aaagtattaa gcctatttgt
     2341 ccttgcgctc tactggcgtc aggacaaaac tcttgattta aattattgtt acgcatacct
     2401 ctctctgata ttttgaaatt aagcataact gatgagccat atgggcgaaa cgtcatatga
     2461 cgtcttatgc aaagccactt taacccttag cctgtggctg gacgttaagt agggtaaata
     2521 aaggtaacat ttttaataat tgtttgatca acgtaaaagc acaggaggta accaattaag
     2581 aaatagctgt aaataggcga ggccgtttgc cttatagcgg ttgatttgcc cccttacttc
     2641 gagcgataca gcattgctgg gtttcaactt tgagtgagcc gctcgaccaa cactaaactt
     2701 a
//
LOCUS       DBNCWK010000211         2687 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.212, whole genome shotgun sequence.
ACCESSION   DBNCWK010000211 DBNCWK010000000
VERSION     DBNCWK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.212"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            188..>2687
                     /gene="rtxA"
                     /locus_tag="AC06IO_002806"
     CDS             188..>2687
                     /gene="rtxA"
                     /locus_tag="AC06IO_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry virulence factor RtxA"
                     /protein_id="HIO9875966.1"
                     /translation="MLVESVIGIVRAVNGLLEKVNAQGQASLVKSGARLEEGDVLTLL
                     SGEAYIQFIHGFPEALALEKPVKLDGVSPTLQNSVEDLKGQMVQEAIAKGIDPSLILD
                     VLGSAAAGAVAVGSGGDAFIIDPLFGFGQVTAGYPTGPISFAYEADTQHLFWYVPEET
                     GVIAESELTSEPESIPQIPQFTTNQAVLIVFEDALASGIPDSAGQARTASSSLSTLLT
                     SSPDVAASFAFNTNLSVLPTLKSGGIDLDYNLSPDKRTLTASIPGGGDVMQFELTAEG
                     QLTQRLMDSIDHPTADSDDSEWMRFDLSSLIDVTFTRASDGAVLESRTLPANAVVAGI
                     QDDVPIARAQLTNNEILLDETVGVKVGDADAANDDYNPTITADPFNNTYGRPIGLVQN
                     ANLLDTSTSEMGGDYKNATMTHLLKITDAVSGLQTSDGTPINLFLESNGDITGRAGDV
                     GAPAVFAIRMNPNTGSITVAQYGSIKQFDTNSHDEAVDLTGRISAVVTAKDSDGDESN
                     AEIPIGQLIIFEDDGPSIDGSKVLSTDVLTVDETNLGVNATASFADNFAQAIDFGEDG
                     AGSVSYALVLNGSNVGSGLYAIDALDVSTADGDGIGRGDEIVLNQNGNVITGSLGGVD
                     YFTITIDAASGEVVFEQLASVWHGNTTNPDDQSALNALANSLVVRATVVDADGDQATH
                     DLDVSQGVFQVKDDGPVASATKLTGVVDEDGLAGGIAGGTGDVAGQAVAALGNVATLF
                     QSGADAPLSYSLNSNTSGLPALSSGGVALTYAVSGGTLTASANGTDVFTFTLGSNGNY
                     TFTLLAKLDHPAGADENDIAINLGSVIRATDSDGD"
BASE COUNT          732 a          550 c          695 g          710 t
ORIGIN      
        1 tactctatcc aatccaggca agtcaatggt atatatcaaa actgttaaag aaaaaaacct
       61 acaaaaacaa gacataatta tatcgatatg attcaaatgt ttacacattt gttacatttt
      121 aactacaagg aggcataatc cttgaactat tattaatatg aataatagta aactttgagg
      181 acagaacatg ttagtagaat ctgttatcgg tatagtgaga gccgtcaatg gattacttga
      241 aaaagtaaat gcgcaaggac aggcaagcct tgtaaagtca ggtgctcgat tagaggaagg
      301 ggatgtactt actttattaa gtggtgaggc ttacatacaa tttattcatg gatttcctga
      361 agccctagcc ttagaaaaac cggtcaagct agatggtgtt tccccaacac tacagaatag
      421 tgtggaagat ttaaaaggac aaatggtcca agaagcgata gccaaaggta ttgatccttc
      481 actcatactt gatgttcttg gctcagcagc agcaggagca gtagctgtag gttcaggtgg
      541 tgatgctttt attattgatc ctttgtttgg tttcggacag gtcacagcag gatatcctac
      601 cggccctatt tcctttgctt atgaggcaga cactcagcac ctattttggt atgtgccaga
      661 agaaacagga gtgattgcag aatccgaatt gactagcgaa cctgaatcta tcccgcaaat
      721 tcctcaattc accacaaatc aagcagtgct aatagtcttt gaggatgctt tagcaagtgg
      781 cattcctgac tccgcaggac aagcaagaac ggcgagttct tctctaagta ctttgctaac
      841 ctcttcacca gacgttgctg cttcctttgc atttaatacc aatttaagtg ttcttcctac
      901 attaaaatca ggtggtatag accttgacta taatttatct cctgataaac gtactttaac
      961 agcatcaatc cctggtgggg gggatgtcat gcaatttgag ttgacagcag agggacaatt
     1021 aactcaaaga ttaatggact cgattgatca cccaacagcg gatagcgatg acagcgaatg
     1081 gatgagattt gatctttcat ctttaattga tgtgactttc acaagagcaa gtgatggtgc
     1141 ggtcttagag tcaagaacac tgccggccaa tgcagtcgta gcggggattc aagacgatgt
     1201 tccaatagca agggctcaat tgactaataa tgaaatccta ttggatgaaa ctgtaggagt
     1261 gaaagtcggg gatgctgatg cagctaatga tgattataat ccaaccatca ctgcggatcc
     1321 ttttaataat acttatggta gacccattgg tctggtgcaa aatgcgaatc tcctcgatac
     1381 cagtacttct gaaatggggg gggattataa aaatgcaaca atgacccatt tgttaaaaat
     1441 taccgatgct gtttcaggat tacagacatc tgacggcact cctatcaatc tctttttgga
     1501 atcgaatggt gatattacgg ggcgagcagg agatgttggt gctcctgcag tttttgcaat
     1561 tcgcatgaat ccaaataccg gctcaattac tgttgcgcaa tatggttcaa tcaaacagtt
     1621 tgataccaac agccatgatg aagcggttga tttgacaggt cgcatttcag ctgtcgttac
     1681 agccaaagac agtgatggtg acgaatctaa cgcagaaata ccgataggac aattgattat
     1741 ttttgaagac gacggtccga gcattgacgg cagcaaggtg ctgtctaccg atgtgctgac
     1801 ggtggatgaa acgaatttgg gcgtcaatgc gacggccagt tttgctgata attttgccca
     1861 ggccattgat tttggtgaag acggggcagg ctctgtgagc tatgccctgg tattgaatgg
     1921 cagcaacgtg ggctcagggc tgtatgccat tgatgccctg gatgtgagca cagcagacgg
     1981 tgacggcatt gggcgaggcg atgagattgt attaaaccaa aacggcaatg tgattactgg
     2041 cagcttgggc ggtgtggatt attttacgat taccattgat gcagcgagcg gtgaggtggt
     2101 gtttgagcag ctggcttcgg tatggcatgg caatacgaca aaccccgatg accagtccgc
     2161 tttaaatgcg ctggccaaca gtttggtggt gcgggctacg gtagtggatg ctgacggcga
     2221 tcaggctacc catgatttgg atgtgagcca gggcgtgttc caggtgaaag acgacggccc
     2281 ggttgcgagt gcaacgaaac tgactggcgt ggtggatgaa gacggcctgg cgggcggcat
     2341 tgccggtggc acaggcgatg tggccggtca ggcggtagcg gctttaggca acgtggccac
     2401 cttgttccaa agtggcgcgg atgccccgtt aagctattct ttgaattcca acaccagcgg
     2461 cttgccggct ttaagttccg gtggcgtggc tctgacttat gcggtgtccg gtggtacgct
     2521 gacggcgtct gcgaatggca ccgacgtctt tacttttact ctgggcagca acggcaatta
     2581 cacgttcacc ttgctggcga agctcgatca ccctgccggg gcggatgaaa acgacattgc
     2641 catcaatttg ggctctgtca ttagggcaac agacagcgac ggggaca
//
LOCUS       DBNCWK010000212         2659 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.213, whole genome shotgun sequence.
ACCESSION   DBNCWK010000212 DBNCWK010000000
VERSION     DBNCWK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.213"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..2009)
                     /gene="ravR"
                     /locus_tag="AC06IO_002807"
     CDS             complement(<1..2009)
                     /gene="ravR"
                     /locus_tag="AC06IO_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavR"
                     /protein_id="HIO9875967.1"
                     /translation="MLSQGVRSMGKEKRELEASYIPLPKKGRFWVHSESCYTEEELNF
                     REWILKIRSNSGIHPDYLNNPSSWFANDDQALVNLYEHAWFYSNAYKPRHVLSLDDFH
                     VQPIDNQRLQWPQLYDLMQKVGDYLLLRNKELPPEHLNQPMNFVLLDVNNILKGLSQN
                     SNIQQVKEQLESVTRYLRTIEKNISPLIGSDRLFLANFRKTVDTKIHPHLDHMLETQL
                     LRDKLGELSKDIRKLSSERNRILHYALNANSVNPHSYEFELDKISDSASYPTQAAKHC
                     AQKSNELVADPLPILHLTKEQLSDCPHFKLITMKDEILDHYAKAVSDLNELERFQKVI
                     QEIMDLLGQAGEVYTVHQFKHQMQSLLTQIDSFIDASSVHIDEIIDANTQAYHKAIQE
                     EQNLPFWKNWLTNEKEKLKIFIKNQDTLSQFPSSSSDLKKTNKALKTRVNEVVSHLNQ
                     PKLKETSFEAIAGQTQALNKLMLSMHDWMKIQHELKGLPLPQPPEPLKIMPTPIPTVD
                     VFSSPPPPLHAYKPLFTALPGNTTAPCMPDNNQCKTTPSSPTQNSLGYLGLIALIPIG
                     VIALYLVIHWMNKKDAQIRVSGTEEEFNKIKEKFEDLLAELKGFEHSEEIDLSDDYYF
                     IVQDYEELIKKTKSNHYDVEALQDTYQELEDLYEEYCNPKKELLN"
     gene            2269..>2659
                     /locus_tag="AC06IO_002808"
     CDS             2269..>2659
                     /locus_tag="AC06IO_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIO9875968.1"
                     /translation="MPSFDIVSKMNEVDLRNAVDNAVREVSTRFDFRGVEATIELKDL
                     TVTLRSESDFQVRQLEDLFRNHCSKRNLSTSGVDIEDEPVHSGKFYTLTMTFKQGIDQ
                     PTAKEIVKYIKETKAKVQTSIQGDKVRV"
BASE COUNT          769 a          444 c          538 g          908 t
ORIGIN      
        1 aagttcagta attccttttt aggattacaa tactcttcat agagatcttc caattcttga
       61 taagtatctt gtaatgcttc tacatcataa tggtttgatt ttgtcttttt tatcaattcc
      121 tcatagtctt ggacaatgaa ataatagtca tctgagagat caatctcttc cgaatgctcg
      181 aaacctttaa gttcagctaa tagatcttca aatttttctt ttatcttatt gaactcttct
      241 tctgtgccag atactcgtat ttgagcgtct tttttattca tccaatgaat aaccaaatat
      301 aaggctatga caccaatagg tatcagggca attaatccta aataacctaa agaattttgt
      361 gtgggtgaac taggggtcgt tttgcattga ttattgtcag gcatacaagg agctgttgta
      421 ttgccaggca gggcagtaaa tagaggctta tatgcatgaa gtggaggcgg gggagaagag
      481 aacacatcaa ctgttggtat aggagttggc attatcttga ggggttcagg tggttgggga
      541 agtggaagcc ctttcaattc atgttggatt ttcatccagt cgtgcattga taacatcaat
      601 ttattaagtg cttgagtttg tccagctatt gcttcaaagg acgtttcttt taacttaggc
      661 tgatttaaat ggctaacgac ttcattgacc cgggttttta aagccttgtt tgtttttttg
      721 agatcagaag aactggatgg aaattgagag agtgtgtcct gatttttaat aaaaatcttt
      781 agtttctctt tttcattagt caaccaattt ttccagaagg gtaaattttg ttcctcttgt
      841 attgccttat gataggcttg agtgttcgca tcaattattt catcaatatg cactgaggaa
      901 gcgtcaataa agctgtcaat ttgtgtcagc agggattgca tttgatgttt gaattgatga
      961 acagtataga cttcgccagc ctgccccaga agatccatga tttcctggat gaccttctga
     1021 aatcgttcca actcgttaag atcagagact gctttggcat aatgatcaag gatctcatct
     1081 ttcatcgtga ttaatttgaa gtgaggacaa tcacttaatt gttctttagt taaatgcagt
     1141 ataggcagag gatctgcgac caattcattt gatttctgag cgcagtgctt ggctgcttga
     1201 gttggataag aagcggagtc actgatttta tccagttcaa attcataaga gtggggattt
     1261 accgaattgg cattaagagc ataatgcaag attctatttc gttcactgga taattttctg
     1321 atgtcttttg ataactctcc caatttatct ctaagtagtt gagtttccag catatggtcc
     1381 agatgcgggt gaattttagt atccactgtt ttgcgaaagt tggctaaaaa taatcggtcc
     1441 gaaccaatca gtggtgaaat attcttttca atagtacgca ggtagcgagt gacggactca
     1501 agttgctctt ttacttgttg tatgttgctg ttctgactta gcccttttaa gatattgttt
     1561 acatcaagaa ggacgaaatt catgggttgg tttaagtgtt cggggggtaa ctccttattt
     1621 cttaataaaa gatagtcacc cactttttgc attaagtcat aaagttgtgg ccattggagg
     1681 cgctgattat caatgggttg gacatgaaaa tcatcaaggc ttaacacatg tctcggcttg
     1741 taggcatttg aataaaacca ggcatgttca taaagattaa ccaaagcttg atcgtcgttg
     1801 gcaaaccagg aactgggatt attaagataa tctggatgaa tccccgaatt ggaacgaatt
     1861 tttaaaatcc attctctgaa atttaattct tcctctgtgt agcaactttc agaatgaacc
     1921 caaaagcgtc cttttttagg taacggtata tatgaagctt caagctctct cttttccttt
     1981 cccatacttc tcactccttg agaaagcaaa aaaaaaccta aggtaattcc agattaacac
     2041 tagattagca atagcttttt ttgctgccga ttaataatat ccataaccaa ctgggtaatt
     2101 tttattccaa tttatttgat aacattgttt gtgcaattat gcaatggtta atcaatcgat
     2161 ttatttttaa acaaccttga tttttgcaag aaagaaaatg gaagtgttgt ttctttcacc
     2221 tatccttgtt ttttgataaa ttacaatttt aatttaaatg gattggttat gccttctttt
     2281 gatattgttt ctaaaatgaa tgaagtggat ttgcgtaatg cagtagataa tgcagtgcgg
     2341 gaagtaagta cccgatttga ttttcgagga gttgaggcta ctattgagct taaagacctg
     2401 acggttactt tgcgttctga atctgatttt caagtgaggc agcttgagga tttattccgg
     2461 aatcattgct caaaacgcaa tctcagcaca tcaggagtgg atatagaaga tgaacctgta
     2521 catagtggaa aattctatac tcttaccatg acttttaaac aaggcattga ccaacctact
     2581 gctaaagaga tagtgaagta catcaaagaa accaaggcca aggtccaaac ttctattcag
     2641 ggggataaag taagggtga
//
LOCUS       DBNCWK010000213         2655 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.214, whole genome shotgun sequence.
ACCESSION   DBNCWK010000213 DBNCWK010000000
VERSION     DBNCWK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.214"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..328
                     /locus_tag="AC06IO_002809"
     CDS             <1..328
                     /locus_tag="AC06IO_002809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875969.1"
                     /translation="MARFSRISLSTVLLSLICFNIFASDTIMITVNTKEKNAIAIGYS
                     VNGNQYGGLGSSYSGHGPKNKEYQFGYKTDSIFGKDISCGSMTLSQDSKVTLVNNNNQ
                     CISVLG"
     gene            594..743
                     /locus_tag="AC06IO_002810"
     CDS             594..743
                     /locus_tag="AC06IO_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875970.1"
                     /translation="MKKIILAVVVISAAVLLSGCGSGCCGASNSCCSYNTGCCGVQNY
                     SGIWY"
     gene            775..1677
                     /locus_tag="AC06IO_002811"
     CDS             775..1677
                     /locus_tag="AC06IO_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIO9875971.1"
                     /translation="MKKNSLLAISEEGFHHIAYTEWGIFEPELPTIICVHGYTRNSRD
                     FDELANYLSSKGRHVFCPDIAGRGDSSWFKNPRHYNFTQYVKDMTALMARTNAHQIDW
                     IGTSMGGIIGMILAAMPNSPINKLVLNDIGPQIPIHGLRRIAKYAGKDPDFKSLEEAK
                     QHFKASYADFGITNEKQWDIFTKNSVEQRDPNLYVTKMDPAIKKSKSILQIISEFFRH
                     PHKALEGIFYDINLWSIWKQIKCPVLVIHGVNSDILTSEIITQMKKTHALTEVYEVEN
                     AGHAPALLDLTVHQRIEDWLATHQ"
     gene            complement(1707..2609)
                     /locus_tag="AC06IO_002812"
     CDS             complement(1707..2609)
                     /locus_tag="AC06IO_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIO9875972.1"
                     /translation="MNSQEQRGSFYAILSGFLYGFIGYFGISAIHGDLSASNMLFWRF
                     LISSIFILIVLIPQLKNINDTYKSMFTAFLSGVLYYGLSTLLYFYASLYIGSGLSMVI
                     FFTYPVIIMVFNYFFYSQTIPKIYYLAISVILIGMALLIDVNEIAFDLWGIFLGLASA
                     FFYACYIVSSKNNEISPNVSTLMVCLGCMTTSLIVACLDQTFKVPTSFPVWLNLFGIG
                     LVATVIPILLMLHSLKYISSEKASILSVLEPVFVVIFGVVLLGEELKPWHALGIVLVL
                     AGALITLFSHRINLNQWLSAIYKS"
BASE COUNT          949 a          470 c          476 g          760 t
ORIGIN      
        1 aatggcgcgc ttttccagaa taagtttaag tactgtattg ttatctctaa tttgtttcaa
       61 catttttgcc tctgacacta ttatgattac agttaatacc aaggaaaaaa atgccattgc
      121 tataggttac tcagtcaatg gaaaccaata tggaggtttg ggcagctcct attctggaca
      181 tggacctaaa aataaggagt atcaatttgg ctataagaca gattccattt ttggaaaaga
      241 tatctcttgt ggttctatga ccttaagtca agacagtaag gtgactttgg taaataataa
      301 taaccagtgt atcagcgttt taggttaatt agctattgca tgaaatatag atattaattt
      361 tgaaaagaca gtagtgccta tccaacaata ttccgctcaa aataattgaa ttccactcgt
      421 agaaactcaa atctgtaatt aacggtttat cccagttaaa gcagtacaga cctctctact
      481 aaaccagact tttagcaact aaaaatacca aaaaacacaa tcaatacaat caattggata
      541 attttagatt catatagtat atttaatata catagaccaa acaaggatag gcaatgaaaa
      601 aaataatatt ggccgtagtt gttatttctg ctgctgtatt attgagtggc tgtggaagtg
      661 gatgctgcgg tgccagcaat tcatgttgca gctataatac tggatgttgt ggtgtacaaa
      721 attattctgg tatttggtat tgatttacaa ttataaaagc caatagtctg aaatatgaaa
      781 aaaaacagct tgcttgccat ctcggaagaa ggttttcacc atattgctta tactgaatgg
      841 ggtatctttg aacctgagtt acctacaatt atttgtgtgc atgggtatac tcgcaattcc
      901 agggatttcg atgaattagc caattatctt agctccaagg gacgtcatgt attctgcccg
      961 gatattgcgg gtcgaggtga tagttcctgg ttcaaaaatc cccgccatta taattttacc
     1021 cagtatgtaa aagatatgac agcccttatg gcaagaacaa atgctcatca aatcgattgg
     1081 attgggacat caatgggcgg catcattggc atgattttag ccgcaatgcc caattcccca
     1141 attaataaac ttgttctcaa tgatataggg cctcaaatac ctattcatgg gttgagaaga
     1201 attgctaaat atgcaggtaa agaccctgat tttaaaagcc tggaggaagc caagcaacat
     1261 ttcaaagcca gttatgctga ttttggaatt acaaatgaga agcagtggga tattttcact
     1321 aaaaacagtg tagaacaacg agaccctaat ctttatgtca ccaaaatgga tcctgcaata
     1381 aaaaaatcaa aatcaatttt acaaatcatt tcagaatttt ttcgtcatcc ccataaagca
     1441 ctggaaggaa ttttttatga cattaatctg tggtcaattt ggaagcagat taagtgtcca
     1501 gttttagtta ttcatggtgt gaattctgat atcttgactt cggagataat cacacaaatg
     1561 aaaaaaactc atgctttaac tgaagtttat gaagtagaaa atgcaggaca cgcaccagct
     1621 ctattggatc tgactgttca ccaaagaatt gaagattggc ttgccactca tcaataaatc
     1681 acaaaagaaa tgccgcatct ttattatcag gacttgtata ttgcgcttag ccattgattt
     1741 aaattaattc tatgactaaa caaggtaatc aaagcgcctg ccaaaaccaa gacgataccc
     1801 aacgcatgcc aaggcttgag ttcctcaccg agcagcacta ctccaaagat caccacaaaa
     1861 acaggttcta aaacggataa gatagatgcc ttttccgaac tgatgtactt caagctgtgt
     1921 agcattaaca aaatcggtat aaccgttgca acaaggccta tgccaaataa attcagccaa
     1981 acagggaatg atgttgggac tttaaaggtc tggtcgaggc aagctacaat caaactggtt
     2041 gtcatacagc ctaaacatac catcagggtt gagacatttg gagaaatttc attgttttta
     2101 cttgaaacta tataacacgc ataaaaaaaa gctgaagcca agcccagaaa tataccccat
     2161 aaatcaaatg cgatttcatt tacatcaatt aacaaggcca ttcctatcag gataacagat
     2221 atggccaaat agtatatctt tggtattgtt tggctataga aaaaataatt gaacaccata
     2281 ataatcactg gataagtaaa aaatatcacc atcgataatc cagagcctat atagagggaa
     2341 gcataaaagt acagtaatgt cgataaacca taatagagga caccacttaa aaaagcggta
     2401 aacatggatt tataggtatc attgatattt tttaattgag gaatcaaaac aatcaaaata
     2461 aaaatactgg aaataaggaa tcgccaaaac aacatattgc ttgctgataa atcaccatgg
     2521 atagcactaa tcccaaaata accaataaat ccgtaaagaa atccggataa aatagcataa
     2581 aaggatccac gttgttcttg ggagttcatg attgagttac tcgaaataag aggtatgatc
     2641 taacaaataa aaagt
//
LOCUS       DBNCWK010000214         2640 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.215, whole genome shotgun sequence.
ACCESSION   DBNCWK010000214 DBNCWK010000000
VERSION     DBNCWK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.215"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            346..1389
                     /locus_tag="AC06IO_002813"
     CDS             346..1389
                     /locus_tag="AC06IO_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875973.1"
                     /translation="MPRARFFKAETNSSHVQQSFINVGGEQFHAAAVALIDYFKRSPR
                     INDDTLKKILERFYFHFPKYIPSQPYLTPSERMSMLINGARKSEIVECFAYVLRQLAI
                     DEIYAKPLNYRDIFAEFDRDTPKSLLRQPSTPLPASALRALSHVIGTNITLSVVENGK
                     ELRKREVYVGDNLNSAKPGITIQVQQNNRYFPAVKNKADFVYVGQLAVSPPKPIESSN
                     TSGKDTIADMVDLIASDNKKLLQTYKQWHQNFLTMLKLGEITTQRMINLYIKFLPSEK
                     GLVVDSTEFFSRLSEDTDKPIIPDYSDENKDGVNQLLASALAGWISARQVDVDQLFDH
                     LENRPSSVSMSVG"
     gene            complement(1484..2434)
                     /locus_tag="AC06IO_002814"
     CDS             complement(1484..2434)
                     /locus_tag="AC06IO_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIO9875974.1"
                     /translation="MIYPNILATIGHTPVVKINRLGKDLECELYAKCEFFNPGGSVKD
                     RIGYEMVVKAEKEGRIKPGDTLIEPTSGNTGIGIALAGAVLGYKVIITMPEKMSQEKQ
                     SVLERLGATIYRTPTEAAYNDPDSHISLAKKLQAEIPNSHILDQYANPNNPNAHYFGT
                     AQEIIDDFGKDLHMVVAGVGTGGTITGIAKRLKEFNPAIKIIGADPEGSILGGGSEVK
                     SYHVEGIGYDFFPDVLDNTLIDAYIKTNDADSFRTARRLIKEEGLLIGGSCGAAMWAA
                     LQAAKSLSKGQKCLVILPDSIRNYMSKFANDEWMKEMGFL"
BASE COUNT          761 a          504 c          548 g          827 t
ORIGIN      
        1 cggcaatttt tgcctaaaat tctcactcaa ttttcgttat tagttccata atttctagac
       61 tggtacagta tagtcatgcc tcataaagtt ggcattattt atgcttgaat gtaatatact
      121 cggcatatac tttctttatt attaaaatcc actgagatga gttgtcagtt tgcagaaata
      181 atcgaacctc aatggcatta tatgaaaatg agcggtgatt taaaaatgat gtggttaaga
      241 gaaacgcccc gtgttaaaag tttcagtaga ctgtaaaaac tttgttaata tttccttaat
      301 ctggattgat tagaatgaag atataagttg ggataggaga acattatgcc aagagctagg
      361 ttctttaaag cagaaactaa tagttcgcac gtacagcaaa gctttattaa tgtgggcgga
      421 gagcagtttc atgcggctgc agttgcttta attgattatt ttaagagatc cccgcgcatt
      481 aatgatgata ctttgaaaaa aatactggag cgtttctact ttcattttcc aaaatacatt
      541 cccagccaac cttatttgac cccctccgaa cgcatgagta tgttgattaa tggggcacgg
      601 aagtcggaaa ttgtcgaatg ttttgcttat gtattacgcc aattggctat agatgagatt
      661 tatgcaaaac ctctcaatta tcgtgatatc tttgctgaat tcgaccgcga cacgccaaaa
      721 tcattgttac gtcaaccatc tactcctctc ccggccagtg ctttgagagc cctttctcat
      781 gtaatcggta caaacatcac tttatctgtt gtggaaaatg gcaaggaatt gaggaaacgt
      841 gaagtgtatg ttggggataa tctgaatagt gcaaaacccg ggataaccat tcaggtccag
      901 caaaataaca ggtatttccc tgccgtgaaa aataaagctg attttgttta tgtagggcaa
      961 ttggcagtaa gcccccccaa accaatagaa agctccaata caagtgggaa agacactatc
     1021 gctgatatgg ttgatttaat tgcgtctgat aataaaaaat tattacagac ctataaacaa
     1081 tggcatcaga actttctaac catgcttaaa ttaggcgaaa taacaaccca gagaatgatc
     1141 aatctttaca tcaagttttt accttcagaa aaaggcttgg ttgttgactc aacagaattt
     1201 ttttcgaggt tatcagaaga tactgacaag ccgattattc ctgattattc cgatgaaaac
     1261 aaggatggag tcaatcagtt attggcttct gcgctggcgg ggtggattag tgccagacag
     1321 gtcgatgttg atcaattgtt tgatcattta gaaaatcgac cttcttcagt atccatgtct
     1381 gttggttaaa gtgtgttggc aatccggatc attttttcaa agcggattgt caacggccat
     1441 tttttgatga aatagcaata cgatagtttt ttatggcttt caattaaaga aagcccattt
     1501 ctttcatcca ttcatcattg gcaaatttgg acatgtaatt acggatagag tcgggtaaaa
     1561 tcaccagaca tttttgtcct ttgcttaagg atttggctgc ttgcaatgcg gcccacatcg
     1621 ctgctccaca tgagccgcca attaacagtc cttcttcttt aatgaggcgt ctggcagtac
     1681 ggaaagaatc cgcgtcattg gtttttatgt aggcatcaat caacgtatta tccagcacat
     1741 cagggaaaaa atcataacct atcccttcaa catgataaga tttgacttct gagccgccac
     1801 cgagaatgga gccttcggga tctgctccaa tgattttgat ggcagggtta aattctttaa
     1861 ggcgtttggc aatgcctgta attgttccgc ctgtcccaac gccagcaaca accatatgca
     1921 aatcttttcc gaagtcgtca ataatttcct gagcggtccc aaaataatgg gcattggggt
     1981 tattggggtt cgcgtattga tcaaggatat gggagttagg gatttcagct tgtaattttt
     2041 tagccagaga aatatgactg tccggatcat tatacgcagc ttctgttggc gttcgataaa
     2101 tggtggctcc cagccgctca agcacagatt gcttttcctg gctcattttt tcgggcatgg
     2161 taataatcac tttgtagcct aatacagcgc ctgccagggc tatacctatg ccggtgttcc
     2221 cggaagtagg ctcaattaag gtatctcccg gtttaattct cccttccttt tctgctttta
     2281 ctaccatttc atagccaata cgatccttta cggagccgcc aggattaaaa aattcgcatt
     2341 tggcatacag ctcacattct agatcttttc caagcctgtt aatttttaca acgggagtat
     2401 ggcctatagt ggccaggatg ttagggtaaa tcatggtagt cctttgtcat ggtgaaatga
     2461 gttgattata agcggataag gtaaaatagc aaaatggggg catctatata ttttctgttt
     2521 tgagtagagc gatatatata aagtcttttt gaggagattc gatttcttat gcgtattata
     2581 aatatgatgg gaggttgact ccggcatgat gaataatcga accatttcca gccatttata
//
LOCUS       DBNCWK010000215         2596 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.216, whole genome shotgun sequence.
ACCESSION   DBNCWK010000215 DBNCWK010000000
VERSION     DBNCWK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.216"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            94..720
                     /gene="lvgA"
                     /locus_tag="AC06IO_002815"
     CDS             94..720
                     /gene="lvgA"
                     /locus_tag="AC06IO_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LvgA"
                     /protein_id="HIO9875975.1"
                     /translation="MADGDIEIKAGFVDTDLDDRKLTMIDDLNNPLAIVERVYLIWWH
                     WADFHLHVISPHIDTITPAIVIEPELISGSNDHEFVYSIHDSGSKLSTSKSQDMFSAG
                     MSMCKLFYTIEKMVHILVDRLKSGGVSMEEEVQIAFAGHEIAQRKAFESIINLPYNVV
                     VTNFDPGIWGEKYLQNVKRLADKGYGYPPESPRENYKHPVSSATTARK"
     gene            complement(750..1127)
                     /locus_tag="AC06IO_002816"
     CDS             complement(750..1127)
                     /locus_tag="AC06IO_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIO9875976.1"
                     /translation="MFFIKYSGALIYDLIIVSILFFAYTSALLLFTHGQAIPPATRWY
                     QLSLFSIAFVYYYGSIRCGGQTVGMKAWRFKLTTESGKKLSRQQIIARFFYFIPATLI
                     APFCLKGSYVLLHQWTQTRFKKV"
     gene            complement(1277..2416)
                     /locus_tag="AC06IO_002817"
     CDS             complement(1277..2416)
                     /locus_tag="AC06IO_002817"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL and HDOD domain-containing protein"
                     /protein_id="HIO9875977.1"
                     /translation="MIAKRPIYNQQLKCVAFEILSCRNVILNEELTRQFYELIYHTDT
                     QLPLFVPFSLKKLLEQFETPINNPIILKLSADEIESVYSLTELQDSLFSIALLINTPQ
                     QLAWLNFAEYIGLTEQLMSKSDVTKVVQYSKAKNRKIIAYDLNQHVNFEKCKAMTMDY
                     YCGDFLLNYIAEEHTEIAANKLNVLQLIQELQKEDCDLNTITHIIQEDPLLSYQLLKL
                     VNSVGFSSGPSIESIDQAVARLGTINLKKWVMLFSMKNISDKPVEILESGLIRAHMAE
                     ELAKKSSNIYSQSAYTAGLLSILDCLLNKPMPALVEQITLAEEIKNALTNKSGPLGNL
                     LSLVIAYEEGHWEEVTQLNINGIDLSQLYIDSLALVSQGTKIMSN"
BASE COUNT          803 a          420 c          493 g          880 t
ORIGIN      
        1 gcttttttac cttttcttaa ggtaggttta atgttctatt gtcaattttg ggctaatata
       61 tcaataatat ccttactacc cgggtaaata taaatggcag acggcgatat cgaaatcaag
      121 gcaggtttcg tagatactga tctagacgat cgcaaactga caatgataga tgatttgaat
      181 aatccattag cgatagtgga gcgagtctat ctgatatggt ggcattgggc agattttcat
      241 cttcatgtta tttctccaca tattgatact attactcctg cgatagtcat cgaacccgaa
      301 ctgatatctg gttctaatga tcatgaattt gtttattcca ttcatgatag tggctctaaa
      361 ttatctacat ccaaaagcca ggatatgttt agtgcgggca tgtctatgtg caaacttttt
      421 tatactattg aaaaaatggt tcatattctg gtagatcgct taaaatctgg cggtgtcagt
      481 atggaggaag aggttcagat agcttttgct ggccatgaaa tagcacagag aaaggctttt
      541 gagtccatta ttaatttacc ttataacgtt gttgtgacta attttgatcc tggaatatgg
      601 ggtgaaaaat atttacaaaa tgtgaagcga ttggcggata aggggtatgg ttatcctcct
      661 gaatccccaa gagaaaatta taagcatccg gtatcttctg caactactgc acgaaaataa
      721 ataatgagcg agaattgctc aacagtgttt taaacttttt taaaacgcgt ttgggtccat
      781 tgatgcaaaa gcacataact cccctttaag caaaaagggg ctattaaagt ggcaggaata
      841 aaataaaaaa atcgggcaat aatttgttgc cttgacagtt ttttaccaga ttcagttgtg
      901 agtttaaatc tccaggcttt catacctaca gtttgtccac cacagcgaat agaaccataa
      961 taataaacaa aagcgatact gaataatgaa agttgatacc aacgggtggc aggagggatg
     1021 gcttgtccat gagtgaataa gagcagggca gaggtgtagg caaaaaataa aatggatacg
     1081 atgattaaat cataaatcaa tgcacctgag tatttgatga aaaacataaa taatacatat
     1141 cctttattga tttcaactat taaattttgg aatattgatc atttaggaaa acacattcta
     1201 aattttttgt tgctcaaata gaagcctggt tcaggcatag aaaaataaaa agaatattgt
     1261 ttaaattgtt gcgcaatcag ttactcatga tttttgtacc ctggcttacc aaggctaaac
     1321 tgtcaatata taattgactt aaatcaattc cattgatgtt aagttgagtt acttcttccc
     1381 aatgcccttc ttcataagca attaccaaag acaatagatt tcctaatggt cctgatttat
     1441 ttgtcagagc attttttatc tcctcagcca gggtgatttg ttctaccaat gctggcatgg
     1501 gtttgtttaa taagcagtct aatatggata ataatcctgc agtataggca ctttggctgt
     1561 aaatattcga acttttttta gcgagctctt ccgccatatg agctctgatt agacctgact
     1621 ccaatatttc tacaggttta tcagagatat ttttcatgga aaacaacata acccattttt
     1681 ttaggttaat ggtgccaagc ctggcaacgg cttgatcgat ggattctatg cttggtccac
     1741 tagaaaaccc aactgaattg accagtttta gaagttgata gcttagtaat ggatcttctt
     1801 ggatgatatg agtgatcgtg tttaaatcgc aatcttcttt ttgtaattcc tggatgagtt
     1861 gaagtacatt taatttgttt gctgctattt ctgtgtgctc ctctgcaatg tagttaagca
     1921 ggaaatcgcc acagtaataa tccatggtca tagccttaca tttttcaaaa ttaacgtgtt
     1981 gatttagatc ataagcgatg attttacggt tttttgcctt actgtattgt actaccttgg
     2041 ttacatccga tttactcatt aattgctcag tcaatcctat gtattcggca aaatttagcc
     2101 aagccaattg ttgaggggta ttaatcagta aggcaataga aaatagagag tcttgtagct
     2161 cagttagtga ataaaccgat tcgatttcat ctgcggataa ttttaaaata atggggttgt
     2221 tgattggcgt ctcaaattgc tcaagcaatt tttttaggga aaaaggcaca aataaaggta
     2281 attgggtatc tgtgtggtaa atgagctcat aaaattgcct tgttaattct tcattaagta
     2341 ttacattgcg acaggataat atttcaaagg cgacacattt taattgttga ttatatattg
     2401 gtcttttggc gatcataaag atctcctttt gctcacatta tgtaatcata gtacggcttt
     2461 ttgtaattgg ccaatggact attcattttc cttataaact gaactttgcg tgataaaaac
     2521 agagtgctat acttcccatg catgaattga aatttagtaa ttctaaatag tttttaagac
     2581 ccaatttttt attttt
//
LOCUS       DBNCWK010000216         2588 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.217, whole genome shotgun sequence.
ACCESSION   DBNCWK010000216 DBNCWK010000000
VERSION     DBNCWK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.217"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..195)
                     /locus_tag="AC06IO_002818"
     CDS             complement(<1..195)
                     /locus_tag="AC06IO_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875978.1"
                     /translation="MVYIKRDKDGRICAVYNQKTSDVTEQIDINDAEFVDFLLRCESD
                     EKLKFLQSDLQLIRVLEDLID"
     gene            complement(411..1307)
                     /locus_tag="AC06IO_002819"
     CDS             complement(411..1307)
                     /locus_tag="AC06IO_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875979.1"
                     /translation="MEPVTIALICAASFGAVVALSAFIRQLLLSRDKNLNDEAQRKAI
                     NQEAGELEKMREQMQSNKRFDSHYKVLGANKDAIIYLDNKIEDILRKKAHLVERYAQV
                     SLKESGAIVEGGISTERKDACDRLKKEIDEEIKFYDSELLHLQQRRTSLWDTHTDLQE
                     YLLGQEKSRNESLDHLYKQHTALLEKIYIRHTDDSENIAKQSIDAGTTSFKAIILAPI
                     QFLLQYFNISSGINFDQSKLENAARDDVDKTESDINDSENSDDEIYSDTDANSDDESN
                     RSDNEDSRDESDSENDSRLIFA"
     gene            complement(1318..1974)
                     /locus_tag="AC06IO_002820"
     CDS             complement(1318..1974)
                     /locus_tag="AC06IO_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875980.1"
                     /translation="MPQRQSEIVVLKPTNLFLSFLASQLPEANLPSLKLLHTDNTAYV
                     IPKHDSDDGTLNEIEKHFTTMFRHEICRWLGRSAHNEIETSFLDFLCCFKFELHSHII
                     LMEPSLKEGHQMLNIKPRSALLEWMKCAVEDQEGLSDVMSRVNLAQIAENSTVIVKNF
                     TTIKDVKPFIKQYFKPIFETTMSRISGQSVQWPQVNSFQSFSRYFAVEIHTQLIHLHY
                     "
     gene            2388..>2588
                     /locus_tag="AC06IO_002821"
     CDS             2388..>2588
                     /locus_tag="AC06IO_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIO9875981.1"
                     /translation="MKLLGKILLVFTALMIFVSITLWILATNIKPETIKQLVSNKITA
                     ITHKQSQIDGTISWQLFPRPGLK"
BASE COUNT          814 a          527 c          376 g          871 t
ORIGIN      
        1 atcaatcaaa tcttcaagta cacgaatcaa ttgcaaatcg gattgtagaa attttaattt
       61 ttcgtcgctc tcacaacgca acaaaaaatc aacaaattct gcatcattaa tgtcaatttg
      121 ctccgttaca tctgaggtct tctggttata tactgcacaa atccgaccat ccttatctcg
      181 ttttatgtag accactgttt aatccttttt tagctaatac tatgtttaga aaaaacagac
      241 acataaatct tatgtctgct gtctctaaac tcaaaccttc catgttaatg gtaagcccaa
      301 acccagacaa aagactttca tgaattattc caggaatatc attaagtaca agcattgtat
      361 tgtaatatat cctttcatag atgtcaccaa ttaactcaaa agattaattt ttatgcaaaa
      421 attagtcttg aatcattttc agaatccgac tcatcccgag agtcttcatt atctgaacga
      481 ttagactcat cgtcagaatt ggcatcggta tcgctataaa tctcatcatc gctattctca
      541 gaatcattga tatcactttc agttttatca acatcatctc gcgcagcatt ctccaattta
      601 gattggtcaa aattaatacc tgatgaaata ttaaaatatt gcagcaagaa ttgtataggc
      661 gccaaaataa tcgccttaaa tgaggtggtt cctgcatcaa tactttgttt tgcaatattt
      721 tcactgtcat cagtatgccg aatatatatt ttttctaaaa gtgctgtatg ttgcttgtag
      781 agatgatcca gactctcatt gcgagacttt tcctgaccca acaaatattc ttgtaaatcc
      841 gtatgagtat cccacaatga agttcgcctt tgttgcaggt gcagcaattc actatcatag
      901 aattttattt cttcatctat ctccttcttt aacctgtcac aggcatcctt tctttctgtc
      961 gagatcccac cctcaactat ggcccctgat tccttgagag atacttgtgc ataacgctcc
     1021 actaagtgag cttttttacg taaaatatcc tcaattttat tatccaggta gataatcgca
     1081 tctttattcg cgccaagaac tttataatga gaatcaaatc gtttattgct ttgcatctgc
     1141 tctctcattt tctcgagttc accggcctcc tgatttattg cttttctctg tgcttcatca
     1201 tttaaatttt tatctctact taataatagc tgtctgataa atgcggataa cgctacaact
     1261 gctccaaacg acgccgcaca gatgagggct atagtcactg gttccatagt caactcctta
     1321 atagtgcaaa tgtatcaatt gggtgtgtat ttcaacagca aaataccggc tgaaagattg
     1381 aaatgaattg acttgtggcc attgtactga ttgaccgctt atccggctca tagtcgtttc
     1441 aaatattggc ttaaaatact gttttataaa tggctttaca tctttgatag ttgtgaagtt
     1501 tttaacaata accgttgagt tttccgctat ctgagccaaa ttcacccgac tcataacatc
     1561 agaaaggcct tcttgatctt caacagcaca tttcatccac tctaaaagcg ctgaacgagg
     1621 cttgatattc aacatctggt gcccttcttt taaagagggt tccattaaaa taatatgaga
     1681 atgtaactca aatttaaaac aacacagaaa atcaagaaaa cttgtttcaa tttcattatg
     1741 tgcagaacgc cccaaccaac gacaaatttc atgtctgaac attgtagtaa aatgcttttc
     1801 gatctcgtta agagtaccat cgtcagaatc atgcttggga ataacataag ccgtattgtc
     1861 agtatgcaat aattttaagc tgggtaaatt agcttccggt aattgtgacg ctagaaagga
     1921 taaaaataaa ttagtaggct tcaacactac tatctcagat tgtctttgag gcatgaggct
     1981 ctccttgatt taattaactc ctcctgatta catctcaact attaaaatca acgtctcatc
     2041 tcaatttaga atcttagtca ctatcttgac taatacagag ttttattcac aaatgtgcat
     2101 gaagtcacac ttgactttca aagacttaaa aattccctga tattgttaaa tttagaacca
     2161 agattaagta catcaaccaa aattctggta atacattaac tcaaaagtcc aaaaaaaaat
     2221 ctctcttatt cagtctaatt tgcatatatg atagtttcaa tagttaaaca gaattttttt
     2281 gagaatattt ttccaatttg ttagaataga cacctatagt cttaaatcct tcttgacaga
     2341 aactccttta tctatatgct ttggttttta aactctggtt ctatcccatg aaattgctag
     2401 gaaaaattct actcgttttt actgctttaa tgatctttgt atcaatcact ttatggattt
     2461 tagcaacaaa tattaaaccc gaaaccatta agcaattggt tagtaataaa atcacagcaa
     2521 tcactcataa gcaaagccaa attgatggga ctatttcctg gcaattgttc ccaaggcctg
     2581 ggctcaaa
//
LOCUS       DBNCWK010000217         2582 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.218, whole genome shotgun sequence.
ACCESSION   DBNCWK010000217 DBNCWK010000000
VERSION     DBNCWK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.218"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..538
                     /locus_tag="AC06IO_002822"
     CDS             <1..538
                     /locus_tag="AC06IO_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875982.1"
                     /translation="SYDLCRFLFDRNLRQYSDRLINHILKDPDYFKLVVGDMGNLLLL
                     AINHPQHADTLINMVIKDKEHFKKLISNRSNWSEQLSHFPKYEKIFANNVPIDENEKN
                     RQLYLANAPHAEIRKNARLFAQAERTHSGQFFFSEAMPRELRIIIAGLTRDSYLCNEE
                     EANQIAQENFSRPMKNSQ"
     gene            complement(579..1013)
                     /locus_tag="AC06IO_002823"
     CDS             complement(579..1013)
                     /locus_tag="AC06IO_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875983.1"
                     /translation="MPFQLPFEKKKNPLNPERLCPSDICFVNLSVFSASELFAGADPE
                     KVIQELTMVNKSYGSAISYNRLPAGSTKDLSEHSKYYLNLIDKAIEDVNLSLDDNSKA
                     TYSLRFALLQPLLVNLVAAAEVLKNEKTQEQEQKSSHSVRFM"
     gene            1276..1956
                     /locus_tag="AC06IO_002824"
                     /pseudo
     CDS             1276..1956
                     /locus_tag="AC06IO_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611931.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          809 a          451 c          479 g          843 t
ORIGIN      
        1 ttcatacgat ctttgtcgct ttcttttcga tagaaattta cgtcaatatt cagataggtt
       61 aattaatcat attctcaaag atcctgacta ctttaagcta gtagttggag atatgggtaa
      121 tttacttctc cttgcaatta atcatcctca acatgcggat acattaatta atatggttat
      181 taaggataaa gagcatttta aaaaacttat aagtaatcga tctaattggt ctgaacagct
      241 cagtcatttt cccaagtacg aaaaaatatt tgccaacaat gtacctattg atgaaaatga
      301 aaaaaaccgc caattgtatt tggcaaatgc tccccatgcg gaaattagaa aaaacgcgcg
      361 tttatttgct caagcggaac gcactcatag tggacaattc ttttttagcg aggccatgcc
      421 tagagaatta cgcataataa ttgctggctt aactcgagac tcctatttat gtaatgaaga
      481 agaagctaat caaattgcgc aagaaaattt tagtcgtcca atgaaaaatt cccaataatt
      541 ttctatggat tccccaactc tggatgaaaa agtaggaatt acatgaatct tacagaatgg
      601 cttgattttt gttcctgttc ttgagttttt tcgtttttca atacttccgc agccgctacc
      661 aggttcacaa gtaatggttg taatagagca aatctgagag aatacgttgc cttgctattg
      721 tcatccagac ttaaattcac atcctcaata gccttatcga taagattaag gtaatatttt
      781 gaatgctctg acagatcttt tgtagagcca gcgggcaatc tattatagct tattgctgag
      841 ccatatgatt tgtttaccat agttaattct tgtatgacct tttccgggtc tgcccctgcg
      901 aataattccg aagcagaaaa tactgacagg ttcacgaaac aaatatctga aggacagagc
      961 ctctctggat ttaatgggtt tttctttttt tcaaaaggaa gttgaaaagg catgacattc
     1021 tccgtttgag tttaaataaa ttatagtata aaaatatcga atccattgct gtgacatttg
     1081 caccagacta atctgtcttt gccttaccat ctctaacaca acctttcttt ggcgctcttg
     1141 cttatttatc aacagcactc ccatacaacc agattacttt ttagttcttc acgaactgat
     1201 aaggtaactg aatgtgacat tttcatagag ttgtaacagc ataaataaaa ccgtaattag
     1261 gtatcaactg ttcctttaag gaaagccctg aatgctcttg gttatccggt tggccgaaga
     1321 aaaacccgta gtttgatgcg tgagtcaggt gtttttgttc gatataaaaa gaaatacaaa
     1381 gtgacaacaa acggcaatca caaacaacct gtttttgaga atgttttaaa caggaagttt
     1441 caagtcaatg aacccaatcg tgcctatgtg tccgatatta cctatatccc aacccatgag
     1501 tgctggttat acctaacggt gataattgat ttgtttttcc gaaaagtggt tggttggaac
     1561 atgagttcga gaatgaagtc tgatacagtt tgcgatgcat taacaatggc catttggcag
     1621 agaaagccaa aagcaggcct tattgtgcat tccgacagag gctcgcaata tgccagtaaa
     1681 caatatcgtg gtcttctcaa tcaacatggt tttgtaggca gcatgagcaa aaaaggtgat
     1741 tgcagggaca atagcgttgc tgaaagtttt ttcagtcgct tgaaagacga gtgagtgcat
     1801 tggcgtaatt atcaaaccag acgggaggca aaacaagata ttctagatta cactactatg
     1861 ttttataaca atcaaagatt gcattcgtct ttggattatc taagtccgaa tcaatttgaa
     1921 agtcggtact gggagttaat gaaaaaagta gcttaactgg ggtgtgcaaa tttgcttgac
     1981 cacgtcacct tcgggtgatt cagcataaat tgataagtca cccccttctc tataaattcc
     2041 taattttaca atatttaata aattcattgc ttcaatatga tcaagtattg attttattgg
     2101 tttgatgtgt gtcattttgt cgatgaataa ttagcgtatt cttaaaattc tggaggatga
     2161 acgctgatgt agatgatcaa cttatatggg gaagtggcgt tttttagtcc gctgcttctt
     2221 ggaagtttat gtaattcttt agggcctaaa gtgttaccta tgtctccggt ataatttgta
     2281 actgatgtct tcggaataga cctaatcaaa tacgcaaatc tcacgatggt tccattttgg
     2341 ttttttatga gagaaattat caatcaatta atcagcaccg gcgaatatta ttgaaattag
     2401 ctgaatttaa ttttatattg cgcatattgt ttaatcgaag acattggatg tattctcacg
     2461 gcactactat acctcactgt atttgataaa tttaagggat tgaatgagaa actttttcaa
     2521 aaatatttca gcgttatatt tagatgttcc aaaactaacg aagactttat tgccaataat
     2581 aa
//
LOCUS       DBNCWK010000218         2579 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.219, whole genome shotgun sequence.
ACCESSION   DBNCWK010000218 DBNCWK010000000
VERSION     DBNCWK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.219"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..305
                     /locus_tag="AC06IO_002825"
     CDS             <1..305
                     /locus_tag="AC06IO_002825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875984.1"
                     /translation="LESIMSIISNHDITFSSTQKDLIEKQQKNNIKNQHIADVKASKG
                     GETSEKLIVFGAKTTALAACGIALLFPLTAPMIIPIAQVSGAVALVIITRKFIKSS"
     gene            complement(485..628)
                     /locus_tag="AC06IO_002826"
     CDS             complement(485..628)
                     /locus_tag="AC06IO_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875985.1"
                     /translation="MKPLNGTIKYLSKDRLDITQNQANQLISAEMTLGDLRLAPKNSR
                     RPW"
     gene            complement(1144..2553)
                     /locus_tag="AC06IO_002827"
     CDS             complement(1144..2553)
                     /locus_tag="AC06IO_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2546 family Dot/Icm T4SS effector"
                     /protein_id="HIO9875986.1"
                     /translation="METKMSITVKSLDFDQCISNRKYKESLQTNDGRKVWDANSLFDA
                     NKEILGKNNNGDPIHVFIGSNRQNLKADLINLNAGAATLFIPVAQELCNIMGATFHPL
                     LVPDLICENATIGDTFHSALQVIKGLNDLNSLNSESLAKLVKSALSGQLNSLHCISDE
                     SKFLMLYSQIQYMAQQYPDEKINFEFYDDKEDILKPLYDIFSKNPDLIPANVTLNIKR
                     YLNGNLMETDFSPILGLGSQQENYQNIVKWIHKQSSSHLKSGNCCQVLEMDNEKITRY
                     CRFGKDETRLKLLDSLENLAKHQVGQKDQKMEGFIKESYEKMGSSKDMDSITLQQSFE
                     EISSAIKVTEAINKVIANYRKEAKCLFSVGMNAKADRIEKALLNVPVEDRGKIFSNDK
                     VSPELIAIRAALASHRYFGKRGNVYYKDEARTVIDENKAATTYNNLRKQFANLRTQSH
                     ADAQVELGHSSEVSRTLKL"
BASE COUNT          779 a          529 c          400 g          871 t
ORIGIN      
        1 ttttggagag tatcatgtcc attatatcta atcatgacat cacttttagt tccacccaaa
       61 aggatttgat cgaaaaacag caaaaaaata acattaaaaa tcaacacatc gcagatgtaa
      121 aagcgagtaa gggaggagaa acatcagaaa aattgattgt ttttggtgcc aaaacaacag
      181 ctcttgctgc gtgtggtatc gctctattat tcccactcac agcaccaatg attatcccca
      241 tagcacaagt cagtggtgca gtggcactag tcatcattac tcgcaagttt atcaaaagct
      301 cctaacagcg cctttagtcg ctagaatgca atgggaagac ttgtattact ttccattgcc
      361 aggaaattca gccattaatt ttaatgatcc tcccataact gaactatatt tactcaaagt
      421 tgaaattaac ctatcagaac atttttccaa acgagagatt ccatcttatt ttatatgtaa
      481 tatttcacca aggtcttctt gagttttttg gtgccagacg taaatcaccc aaagtcattt
      541 ctgcagaaat gagttgattg gcttggtttt gagtaatatc caacctatcc tttgagagat
      601 attttattgt gccattaagt ggtttcacaa tgaaacacct cactgaatta aatttgataa
      661 atggtcatca aatatcaaat cttcctttaa attaaatcgt tataaaggcg tgtttccttg
      721 ccacctgatt actctctaca aacggaggga atcacctttc gcggagtatc aaaaacaatt
      781 gctaacacta tacccctttg tctgcattaa atacgagctc tttcttattg tcatttttga
      841 acccagtaat gtatcgacat gtggtcaccc aaattccggg tcaaatttct ctaaaacttt
      901 taaatgagct aaaccttcgt tttgaaccat ttcattttgt atggactcac agcttaaacc
      961 tatctcactc aaataaaaaa ttggcaatcg atacataaat ctctcagatc tcttctatca
     1021 aatattacct tattatcgca acgaacattg cttaaaaatg acagtttaat aagcttttgg
     1081 tagtaataaa acaaaccgga actgtcgaac aaatccggct ggaatagaaa tgtatagaac
     1141 catttatagt tttagagttc ttgaaacctc agacgaatgc cccagctcaa cctgtgcatc
     1201 agcatggctt tgtgtcctta aattcgcaaa ttgctttctt aaattattgt atgtcgtcgc
     1261 cgctttattt tcatcaatca cggtacgtgc ttcatcttta taatagacat tcccccgctt
     1321 cccaaaatag cggtgtgaag ccaaagccgc tcttatcgca attaattccg gagaaacttt
     1381 gtcattagaa aaaattttac ctcgatcttc cacgggaacg ttgagtaatg ccttttctat
     1441 tctgtccgct ttggcattca tgccaacgct aaataagcat ttggcctctt tgcgataatt
     1501 tgcaatgact ttgttaatcg cttcggttac ttttattgca ctactaattt cctcaaatga
     1561 ttgttgcaac gttatagaat ccatatcttt tgagctaccc attttttcat aagactcctt
     1621 gataaagcct tccatttttt gatctttttg acccacttga tgttttgcaa gattctcaag
     1681 agaatctaat agttttagcc ttgtctcatc ttttccaaat cggcagtatc ttgtaatctt
     1741 ttcattatcc atctctaata cttggcaaca attccctgac tttaggtgac tgcttgattg
     1801 tttatgaatc cattttacaa tattctgata attctcttgt tgagacccca ggcccaaaat
     1861 tggactaaag tcagtctcca taagattgcc attcaaataa cgtttaatat tcaaagtcac
     1921 attagcaggt attaaatcag gatttttaga gaagatgtca tacagtggct ttaatatatc
     1981 ttccttgtca tcgtaaaact caaaattaat cttctcatca ggatattgtt gagccatata
     2041 ctgtatctgt gaatacagca ttaaaaattt actctcatcg cttatgcaat ggagtgaatt
     2101 tagttggcca ctgagcgccg attttaccag ttttgccagc gactcactgt ttaatgaatt
     2161 caagtcattc aagcctttaa tcacttgcag agcactgtga aaagtatctc caattgtggc
     2221 attctcacag attaaatcag gaacaagtaa tggatgaaaa gttgccccca tgatattgca
     2281 caactcttga gctactggta taaataaagt agctgctcct gcatttaaat tgattaaatc
     2341 cgctttgagg ttttgcctgt tagaaccaat aaaaacatgg atgggatcgc cattattatt
     2401 tttacccaga atttccttat tggcgtcaaa taaactgtta gcatcccaaa ctttcctccc
     2461 atcatttgtt tgtaatgact ctttatattt cctgtttgag atacattgat caaaatctaa
     2521 ggatttaact gttatgctca ttttcgtctc caaggttaga ataattaccc tttattgtt
//
LOCUS       DBNCWK010000219         2501 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.220, whole genome shotgun sequence.
ACCESSION   DBNCWK010000219 DBNCWK010000000
VERSION     DBNCWK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.220"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            276..707
                     /locus_tag="AC06IO_002828"
     CDS             276..707
                     /locus_tag="AC06IO_002828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875987.1"
                     /translation="MGPKLMCGLTMFFVSAVAMANLSSTVETRLGDNNQLIIPFTLNN
                     QGTQAITSESNLVPSEAKVVAPQDAVMFEVTLPDVVGTQRIRYHAGTSGCDFYIDIQN
                     GGASPDSHVTYVSAVPINGITGCNVYSSGGVNHLAVSFTQF"
     gene            1328..>2501
                     /locus_tag="AC06IO_002829"
     CDS             1328..>2501
                     /locus_tag="AC06IO_002829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875988.1"
                     /translation="MHNKILQLVNSDQQEKSISRKLIKWHLKDPTLFNLTFECKRSLF
                     YVLVRKNKVVVIEELLKIDKFITLAMKADRNHDSILHYSIRKPKNNRLSFLFIDRLPD
                     LLNLRNSLGNTPLHEAVINENIEVIYTLAKHPNIKIQMNYMLKTPFDLIYHNDEKVKH
                     ALCSEEINRIMALDLSHTLLSDLSRGVGKSFPGLTSQNPSSCHLTSEKATTHLELSDA
                     PLTTSSKGKETKQKSSFSKKKNKKKLSEIFEQLVMAYEQNRYSLEYQELKNQFCMLCL
                     KTRSVNVQNLYNSIRSAPNNKAILRDILNQLYPIMHGKLAKNLEKKMKVKEAILVLII
                     SEIHKSSFQSNSIDFTPIKEMNQDLLEIIRLILTPQEMAYGVHQYDLIKISHQVVGL"
BASE COUNT          828 a          450 c          435 g          788 t
ORIGIN      
        1 atgggccaag gcccgaccta cgcttgcttc cttttgttaa tcaggggaaa actgcttttc
       61 tagaagaaga ggcattttct tacggattaa cagatttcct aacaggcgtt tatcctgata
      121 aagttgaggg atttatgtta aataatgaag ctcacattat tctttatttt attatctaga
      181 gtccatatgg tttaaccaaa aaaataacaa agtgattgaa aaaaatacta ataaaagtta
      241 atatatgaac cttgttgaat tttggagata tatttatggg tccaaaacta atgtgtggat
      301 taaccatgtt ttttgtttct gctgtagcaa tggctaatct atcgagcacg gttgaaacac
      361 ggctaggtga taataatcaa ctaatcattc cgttcacttt gaataaccaa ggcacccaag
      421 ccatcacttc agaatctaat ttagtgccca gcgaagctaa agtagtagcg cctcaagacg
      481 cagtaatgtt tgaagtgact cttcctgatg tagtaggaac acagcgcata cgatatcatg
      541 ctggtacctc aggctgtgat ttttatattg acatacagaa tggcggagct tcacctgaca
      601 gccatgtgac ctatgtttct gctgttccta ttaatggcat tacaggatgt aatgtttact
      661 cttctggtgg cgtaaatcat ctggctgtat catttacaca gttttaagat ttttataagc
      721 ccttctacat attgtgttgg ctcaaaaata atgaggtcaa caccgcttcc accaaatcac
      781 cctaatacat cgtttaatag tattaaacgt gaattcgata taactgctca aacaacagcc
      841 ataaaagatc gttgaatatt taaagagggc ttctttggtt taagtgtata agccgctaaa
      901 ccggccaaca tgacgtaata gtatggatcc ctcaccttga ttgaatgact gttaacatat
      961 tcattctggc tcaaatagaa gcctttcgat aggtggcggg gtccatacta ttacgtcaaa
     1021 cttcacgtat tttagtgaag atgtgatacc ccaaagctca tgtggttttt catggtcttt
     1081 gatggttttt cgtgattccc gctcaaatgt atggtaaagg gttcgattcc tgctatcact
     1141 aacattccaa tatagtcagc tccattccaa aaatccttgt aaaaacttga ggttatcttt
     1201 attatcggtc gcctgttgct ctgtgtatat cgggctttat agctcttaat atcaggtgag
     1261 ttttgttcga tatcattgaa cctgtctaaa atctggctgg atgtggattt aaaaaaggag
     1321 tttatgaatg cacaataaaa tacttcagtt agtcaactct gatcagcaag aaaaatctat
     1381 ctccagaaaa ctgattaaat ggcatcttaa agatcctact ttatttaatt tgacctttga
     1441 gtgtaagcgt tctctttttt atgtcttagt taggaaaaat aaagtcgttg tcattgaaga
     1501 gttgttaaaa attgataaat tcataactct tgcaatgaaa gccgatagga accatgattc
     1561 cattttacac tattcgataa gaaagcctaa aaataatcgc ctctcttttt tgtttattga
     1621 ccgtttgccg gacttattaa atctacgcaa ctctttaggc aatactcctt tacatgaagc
     1681 tgtaatcaat gaaaatattg aggttattta taccctcgcc aagcatccaa atattaaaat
     1741 tcaaatgaat tacatgttaa aaaccccatt tgacttgatt tatcataatg atgaaaaagt
     1801 aaaacatgca ctatgtagtg aagagattaa ccgtatcatg gctttggatt tatcacacac
     1861 tttactttcc gatctttcaa gaggggttgg gaaatcattt cctggattaa catcacaaaa
     1921 tccatcaagc tgtcatttaa catctgaaaa agccactact cacttggagc tttctgacgc
     1981 accattaacc acgagttcaa aaggaaaaga gacaaaacaa aaaagcagct ttagcaagaa
     2041 aaaaaataag aaaaaattaa gtgaaatttt tgaacaatta gtcatggcat acgaacaaaa
     2101 tcgttattca cttgagtatc aagaactaaa aaatcaattt tgcatgcttt gcctgaaaac
     2161 aaggagtgtt aatgttcaaa atctatataa ttcaataagg agcgcgccaa ataataaggc
     2221 aattctacgt gatattttga atcagttata tccaataatg catggaaaat tggctaaaaa
     2281 tcttgaaaaa aaaatgaagg tgaaggaagc aatacttgtc ttaatcattt ctgaaattca
     2341 taaatcctcc tttcaaagta attccatcga ctttacgccg ataaaagaga tgaatcagga
     2401 tctgttagaa atcattaggc taatccttac gcctcaagaa atggcttatg gggttcatca
     2461 atatgaccta ataaaaatct cgcatcaagt cgttggactc c
//
LOCUS       DBNCWK010000220         2481 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.221, whole genome shotgun sequence.
ACCESSION   DBNCWK010000220 DBNCWK010000000
VERSION     DBNCWK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.221"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            115..855
                     /gene="ravK"
                     /locus_tag="AC06IO_002830"
     CDS             115..855
                     /gene="ravK"
                     /locus_tag="AC06IO_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavK"
                     /protein_id="HIO9875989.1"
                     /translation="MVSLEHIQKLISECRKLGKDGLDNGTNGLIPELEIDVVPPSAFL
                     GVGNNPAIFVNSKTYKLMRTTHEKWVENKTIVFKSYLLSQPAIKIIGAIVHETGHAFN
                     VAAKIPNTEANACIFEIEVLMRLFQVKSPLLLGCTELDMQSYFKSRLTDYNKCVKDCR
                     CLAEMIQLITHQFKLDEVSISEKENQIPLLSISNKWPGLFAKKQIAPDMDKLLTSPVT
                     ITPEVKILFYQLVKEHFHSPETEIKLDI"
     gene            complement(958..2421)
                     /locus_tag="AC06IO_002831"
     CDS             complement(958..2421)
                     /locus_tag="AC06IO_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIO9875990.1"
                     /translation="MKTNIPTLSKPIKDSGYRRGLKDRHVQLIALGGIIGSGYFLGTG
                     EVINLVGPSVFIAYLLGGLIIYLTMLCMGELAVAIPISGSFVTYTSDFISPTVACGVG
                     WSYWITWVAYIPAECVAGGIIMELFTGVSGYIWVVCFGLIITYINLAKVDTFGEIEFW
                     LALIKIISLLAFVFLAILIFFGLIHGSEPPGIIGFKYLLGDGGLLPNGAMSLLTAMVL
                     LLVNYQGSEIIGLAAGESENPARMIPHAIRNVTFRILFLYIIPVFCLVLIFPWQKAGL
                     SNSVFADALNFYGLKWAGAVTSFVTLSATLSCANSGFYGAVRSLNALARDGMAPHVLA
                     KFNQNSVPQNAVIATLIGVWILLGVGYFFGQTKLYIALLLVSGFTGTLAWLSLCIAQI
                     SFRNRLYKAGYSIKDLRYVTPYSPYTGILAVILMVGSLFFLLLNKDPIYKLSFIIGVV
                     SFIIPVIIYKVFDLSKVRKKALHVKSRVKFQDLFPPQ"
BASE COUNT          853 a          454 c          479 g          695 t
ORIGIN      
        1 tgactgaata atttcaatac caaataatgg tctgatgtga agcatatttt gatcagtttg
       61 ttatctttga tttctttatg atcgcaggga tttcttacaa taaggagggg tattatggta
      121 agtttggagc atatacaaaa acttatttcg gaatgtagga agttgggtaa agatggactt
      181 gataatggta ctaatgggtt aatccctgaa ttggaaatcg atgttgtccc tcccagtgcg
      241 tttttagggg taggtaataa tccggcaatt tttgtaaatt ctaaaacata caagttaatg
      301 cgtacaaccc atgaaaaatg ggttgaaaac aaaacaatag tttttaaaag ttatttgctt
      361 tcccagccgg cgatcaaaat tattggcgct attgttcatg aaactggtca tgcttttaat
      421 gtggctgcca aaattcccaa tactgaagca aatgcctgta tttttgaaat tgaagtgtta
      481 atgaggttgt ttcaagttaa aagccccttg ctgctagggt gtacggaact ggatatgcaa
      541 tcttatttta aatcacggtt aactgattac aacaagtgtg tgaaagactg ccgatgttta
      601 gctgaaatga ttcaattaat aactcatcaa tttaagttgg atgaggtatc aatttcagag
      661 aaagaaaatc aaatcccgct tctgtctatt tcaaacaaat ggccaggctt atttgcaaaa
      721 aaacagattg cccctgacat ggataaatta ttgacttctc ctgtaactat cactccagag
      781 gttaaaatcc tgttttatca attggttaaa gagcattttc attctcctga aacagagata
      841 aagcttgata tataacaata tttttgattg atgcaggtaa attactgtga ctttatgagt
      901 ctggcatgca aaatgaattc tgcacgccag aaaacacttc aaaaagtaat gtttaattta
      961 ctgtggcgga aataaatcct gaaatttcac tcgacttttc acatgcaaag cttttttcct
     1021 aaccttggat aaatcaaaga ccttatagat gatgacgggt ataatgaaac tcaccacacc
     1081 aataataaat gataatttat agataggatc tttatttagc agtaagaaaa ataaggagcc
     1141 aaccatcaaa atgacagcaa gaattccggt atagggtgaa taaggtgtta cataacggag
     1201 atctttaatg gagtaaccgg ctttatacaa acggtttcta aagctgattt gagcaatgca
     1261 taaagaaagc caagccagcg ttccagtaaa accggatact aataataaag cgatatagag
     1321 tttcgtttga ccaaaaaagt aacccacacc taaaagaatc caaactccaa tcagtgtggc
     1381 aataacggcg ttctgaggaa cagagttttg attaaatttg gctaaaacgt gtggagccat
     1441 accatcacgc gccaaagcat ttaaagaacg tacagcacca taaaatcctg aattagcgca
     1501 ggataaggtt gcagacagag taacaaaact cgttactgct cctgcccatt tcaggccgta
     1561 gaaattaagc gcatcagcga agactgagtt ggataaccca gctttttgcc agggaaaaat
     1621 taaaaccaga caaaatactg gaatgatgta gagaaacagg attctgaaag taacattacg
     1681 gatggcgtga gggatcattc gggcagggtt ttcagattct cctgctgcaa gtcctatgat
     1741 ttcagatcct tgataattaa ccagcaaaag aaccatggct gtcaacaacg acattgcccc
     1801 attaggtaac agacccccat cgcctaacag gtacttaaat cctattatac cgggtggttc
     1861 tgatccatga atgagtccaa agaaaattag tattgccagg aaaacaaagg cgagtaatga
     1921 gataatttta attaaggcga gccagaattc aatttcacca aacgtatcga ctttagcaag
     1981 gttaatatag gtaataatta atccgaagca aaccacccat atgtaaccgc tcaccccagt
     2041 aaacaattcc attatgatcc ctcccgcaac acattctgcg ggtatatagg cgacccaggt
     2101 aatccaatat gaccacccga caccgcaagc gacagttggg gatataaaat cagaagtgta
     2161 agtaacaaaa gagccagaaa ttggaatggc gactgctaat tctcccatac ataacatggt
     2221 taggtaaata attaacccac caagtaaata ggcaataaac acagaggggc caacaagatt
     2281 aattacttca ccggttccta aaaaatatcc agagccgata attccaccta gtgcaatcag
     2341 ttggacatga cgatctttta aacctcgtct gtaaccactg tctttaatgg gttttgatag
     2401 ggttggaata ttagtcttca cgggctgttt tttctcacta tgttagtaaa aaataataga
     2461 gctaatgtta tatttatcca t
//
LOCUS       DBNCWK010000221         2452 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.222, whole genome shotgun sequence.
ACCESSION   DBNCWK010000221 DBNCWK010000000
VERSION     DBNCWK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.222"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(102..716)
                     /gene="lem15"
                     /locus_tag="AC06IO_002832"
     CDS             complement(102..716)
                     /gene="lem15"
                     /locus_tag="AC06IO_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem15"
                     /protein_id="HIO9875991.1"
                     /translation="MIYKITLTDISLAQAFISIPQDVIALDLSNNLLGSKSCVDLLVN
                     IADCIPAGVTSLDLSSNGLRLTQVSLVTPKHVTSLNLCFNPLHEISIDGLEWLKDSLA
                     HIQIIYLSYDMVNNMSREQRQAFRAVIPNIQTIILVDKNGKEIGPSHTAKIANLIREL
                     GGRNPEVPSLLNQCTFFVKTQGINIDKAYIPEELKKRIHDCSPV"
     gene            complement(866..2157)
                     /locus_tag="AC06IO_002833"
                     /pseudo
     CDS             complement(866..2157)
                     /locus_tag="AC06IO_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444375.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
     gene            complement(2144..2425)
                     /locus_tag="AC06IO_002834"
     CDS             complement(2144..2425)
                     /locus_tag="AC06IO_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9875992.1"
                     /translation="MAKQIKKLKTPNPQQPLTVELLGEVIKARRTQSNLRLEDAAALC
                     GVAKETFMKIEHGQSNCQLASVLQICSGLGISLYIKPWMDNGEDENDWG"
BASE COUNT          780 a          477 c          397 g          798 t
ORIGIN      
        1 atgtaagcat taaaactcct ctactgaaaa gcaattttaa tgcttagctt gacggctatg
       61 ggctaaggcc cgacctacac ttgctactac gcaataataa atcagacagg actgcagtcg
      121 tgaatacgtt tttttaattc ttcgggaata taggccttat ctatatttat tccttgagtt
      181 ttaacaaaaa aggtacattg gtttaacaaa gagggaacct ctgggtttct acctccaagc
      241 tctctaatga gattagcgat ttttgctgta tgcgaaggac caatttcttt gccattttta
      301 tcgaccaaaa taattgtttg tatgttggga attaccgctc gaaaggcctg tcgttgctct
      361 cttgacatgt tattgaccat atcatagctt aaataaataa tttgaatatg tgctaacgaa
      421 tccttaagcc actctaaacc atcaatagat atttcatgta agggattaaa acacaaatta
      481 agtgaagtca cgtgtttggg tgtaactaaa gagacttgcg ttaatctgag cccattgcta
      541 ctcaaatcaa gcgaggtcac acccgcaggt atacagtccg ctatatttac taataaatct
      601 acgcaacttt ttgagcccag gagattatta ctcaaatcaa gggcgattac atcttgaggg
      661 atagatataa aagcttgtgc caagctaatg tcagttaatg ttatcttata aatcataatt
      721 aaaaccccat tataaatgaa aaaatattat tcttattggt cataatatga tatatttgat
      781 tataattaaa atagattgtt tgtatattaa atatacaaat ctcttatgtg ctccgtatta
      841 aagtaagaaa taatgaggaa aggctctaga gctttattga agtaatttgg tcgttctgct
      901 ttaacaagtg ttcacatctc ttcatgacaa tttcctgata tttcttaaaa tagtcttctt
      961 cgttaccaac taaattaaat tgggtttctt cttgcaaagc agttgttaat ttgccaatca
     1021 aatattttaa atgtttagct actaacgacc ttggcagcct acatgtatct gcaaaatcag
     1081 ccaactgata agcattaata ttatcccatc aaattcatcc cctaatgcca tcgccagagc
     1141 atgctcaaat tcaggataca tttttatatt aaccaaatca taaaatggcg ccagtgatat
     1201 accgttaact ccaacaaaaa aactgatatt tttaccgtga gcgccacaat taaatatcaa
     1261 aacattaaat aaaacccagt cgagtatttg ttgcttggtt ttggcaggat tgatacattg
     1321 attggcaaaa tcaaatagct tgggcaaact gactccatct ctaatatgag ccacatccct
     1381 gttatttcca aaattttgct catatttata ttcagggggt aaattcaatg cctgacaccc
     1441 gtctattaaa tgacgcctca ccacattagt ggtaacccat ttcctgtcaa aacgctctac
     1501 caataaagca gggttctgac caaaacgctt catctgaaca ttggctacat tcaattcaca
     1561 gcatttagcg agccacatac tgaggaattc atttaaaacc agataagata gtttttgtgt
     1621 ttcaaattta agaatatggg tagaacacaa gctaccttct ccaaaaccaa gttgtccttc
     1681 tttatttaac accacattaa ttttatcttg tactccggca accgaaagac gtggttttcc
     1741 gtcccaaacg attaaattgt aagaatcaca atgatttaaa cgatcttcta attcttcatg
     1801 agttactagt cgaaaattag catgtttagg aattgtttga tttgatggca atataactaa
     1861 tgccccaggt gtatccaacc ctaaagcttg aataagtcca aatgtattgt atttacttaa
     1921 atgaaaaaca ttaactagta cttctaaagg atttccttcg ggtaataaat ttcttagaaa
     1981 tcgttgcaca tttaatggtg gaatatcttc atgcatggga aggtgtgggg aaattgcgta
     2041 tcctgtctgt tgccaactgt catgataact ccaatacagc tgatcctcga taagcgtgat
     2101 attggctatt tgacgttctc cgagataaac atccaagact ggcttatccc cagtcatttt
     2161 catcctcacc attatccatc caaggtttta tgtaaagact tattcctaaa ccagaacaaa
     2221 tttgtaatac acttgccaat tgacagttcg attgcccgtg ctcgattttc ataaaggttt
     2281 ctttggcaac accacataaa gcagccgcat cttctaaccg caaattgctt tgggttcgac
     2341 gtgctttaat gacttcccct aacaattcca cagttaatgg ttgttgaggg ttaggagtct
     2401 ttaatttttt tatttgctta gccatataat cctactagag tagacttaat ct
//
LOCUS       DBNCWK010000222         2422 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.223, whole genome shotgun sequence.
ACCESSION   DBNCWK010000222 DBNCWK010000000
VERSION     DBNCWK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.223"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(41..676)
                     /locus_tag="AC06IO_002835"
     CDS             complement(41..676)
                     /locus_tag="AC06IO_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIO9875993.1"
                     /translation="MFLQTSQKLPKGIFKACVVPRPIAWIVTKDPEGNHNLSPFSFFN
                     IVCEEPPMVMFATTGSHNSGGAKDTLKNVEDTKEFTVNLVSYGSKEAMNITSLGFSRG
                     ISEFDMAEVDHLPGRLIQTPRVKESPISLECVYHESIQLPIPIHSNLINRMVIGNVVG
                     IHLKPEILTKDKRIDTNKLNLIARVGYNSYTKVKAHFDLNRPCGCCNKLMD"
     gene            845..2191
                     /locus_tag="AC06IO_002836"
     CDS             845..2191
                     /locus_tag="AC06IO_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875994.1"
                     /translation="MFTTIDNNEGVVQTYWAEVRNKFQKADPYLSKLIDNVSPDKLPI
                     YLLYFPYGMLKGDTKSSYMPLLDGGYIKLSDTEVDKKIINDLGYGMYSSPLGIVLDKF
                     IEYFIEFDDKVFTYYISGPGTIFNTGMLLKNKNSRNYSPNGVLKATAGARTAFMLPSI
                     NSHNGINKLSKLVNQDLTTPRNHNDHFELFKAINQHDSSNWKVCLAYFSEKWVKHLLT
                     DPAWVEIKNYMLEAKNKNDSFSVNSAYYDIFYSKAQKDRNLRTSSPYLTNTAIHLIKI
                     ALGEHPGYVPATTANFLPIESIQKFISESFQLKRTPTIMIPHSLVYEKEKEPVYYSLQ
                     NPTTPHFLTKKNEKVTANQEIDIIYRILNKFIEEMSKQDSLLAGTVFSDISDHIRFNY
                     FHNYPPKDSNLINNSRQLSKLDPRFNFSSYKNGESEFCFEGQFLRGCIQIQPNVKE"
     gene            complement(2181..>2422)
                     /locus_tag="AC06IO_002837"
     CDS             complement(2181..>2422)
                     /locus_tag="AC06IO_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIO9875995.1"
                     /translation="KSIIQNDKDLVDSIERKIAICQLQRKLLSWYNEMHPIVLYVASI
                     YPQVPCEKFDICLSNLDKVNIPRLATAYIKPDLIP"
BASE COUNT          830 a          408 c          372 g          812 t
ORIGIN      
        1 tgtattagcc tctaacttat ttattttctt attcataatt ttaatccatt aatttattac
       61 aacatccaca aggcctattt aaatcaaaat gagctttgac tttggtatat gagttatatc
      121 cgactcttgc aatgagatta agtttattgg tatcaatgcg tttatcttta gttaaaattt
      181 ccggtttaag gtgaattcca acaacattac cgattaccat tcggttaatt aaattagaat
      241 gaattggtat tggtaattga attgattcat gataaacaca ctctaaactg ataggtgatt
      301 cttttacccg aggtgtttga atcagcctac caggtaaatg atctacctca gccatatcaa
      361 attcgctaat tcctcgcgaa aaacccaggg aagtaatatt cattgcctct ttacttccat
      421 agctgaccaa gttaactgta aattctttag tatcttcaac gttttttaga gtatctttgg
      481 caccgccact gttatgagat cctgtcgtag caaacataac cattggaggt tcttcacata
      541 caatgttaaa aaaactaaaa ggcgataaat tatgattacc ttcaggatcc ttggtgacaa
      601 tccaagcgat aggccttgga acaacacaag ctttaaatat tccttttggc aatttctgtg
      661 atgtctgtag aaacatggtg atatattcct ttatttaaat ttccccaacg ctgtttttaa
      721 attgggatgc ttgacgctac agattcaatc atgattgact tttcagtaaa actactttta
      781 aaatggataa tattaagtgt tataaaaaac cctttttata atgggttttt aacaaggatg
      841 tttgatgttt accacaattg ataataacga aggtgtagtc caaacatatt gggcagaagt
      901 aagaaataag tttcaaaaag cagatcccta cctttctaaa cttatagaca atgtctctcc
      961 tgataagtta ccaatttact tactttattt tccatatggc atgttaaaag gtgatacaaa
     1021 aagctcttac atgcccttgc tcgatggagg ttatattaaa ttatctgaca cagaagttga
     1081 taaaaaaata atcaatgact tagggtatgg aatgtactca tctccattag gcatagtact
     1141 tgataagttt attgaatatt ttattgaatt tgatgacaaa gtttttactt attatatctc
     1201 tgggcctggt accatattta atacaggaat gcttttgaaa aataaaaatt caagaaatta
     1261 tagtccaaat ggcgtactaa aagcgactgc aggtgcacga acagctttta tgcttcccag
     1321 cataaacagt cacaatggca tcaataagct ttctaaactt gtcaatcaag atctaacaac
     1381 cccgagaaac cataacgatc actttgaatt atttaaagct ataaaccagc atgatagtag
     1441 taattggaaa gtttgccttg cctacttctc agaaaaatgg gtgaaacatt tgcttactga
     1501 tcctgcttgg gttgaaatta aaaattatat gctagaagct aaaaataaga atgattcttt
     1561 tagtgtgaat agtgcatatt acgatatttt ttattctaaa gcccaaaagg atagaaatct
     1621 tcgtacatct agcccatatt taacaaacac agccatacat ttgatcaaaa tcgctcttgg
     1681 tgaacatcct gggtatgtac cggctacaac tgcaaatttc ctacctatag aaagcataca
     1741 gaaatttatt tctgaatcct tccaattgaa aaggactccc actataatga ttccacactc
     1801 actagtgtat gagaaagaaa aagaaccggt atattattca ttacaaaacc caacaacacc
     1861 gcattttctt actaagaaaa atgaaaaagt tactgcaaat caagaaattg atattattta
     1921 tagaatttta aataaattca ttgaagaaat gtctaagcaa gactctctac ttgcaggcac
     1981 tgtattttct gatatttcag atcatattag atttaattat tttcataatt accctccgaa
     2041 ggatagcaat ctgataaata attctcgtca gctttcaaaa ttagatccaa ggttcaattt
     2101 tagtagttac aaaaatgggg agtctgagtt ttgttttgag ggtcaatttt tacgaggctg
     2161 tatacaaatc caacccaatg ttaaggaata agatcaggtt ttatatatgc tgtggccaac
     2221 ctaggtatat tcactttatc taaatttgaa aggcaaatat caaatttctc acaaggaact
     2281 tggggatata tagatgcaac atagagaaca ataggatgca tttcattata ccaactaagt
     2341 aattttcgtt gtagttgaca tattgctatt tttctttcaa tactatcaac taaatcttta
     2401 tcattttgaa taattgattt aa
//
LOCUS       DBNCWK010000223         2335 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.224, whole genome shotgun sequence.
ACCESSION   DBNCWK010000223 DBNCWK010000000
VERSION     DBNCWK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.224"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            89..991
                     /locus_tag="AC06IO_002838"
     CDS             89..991
                     /locus_tag="AC06IO_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9875996.1"
                     /translation="MFSPLQDPWSELEKLGFNPDYLERLKKRAATSKELAVNVEMLAD
                     YYERFKAAGFTDRDLARIVSLVSFTSDNRLSNIGSRMMLTLVCGGLDLLKAPLNFNSH
                     NLTNMMVSLNGLEKLRQLVKINQSHIIPFKNQAEFKAVFEKKCKKYPMLRVVWCYSDK
                     EELYLENRRQDAIYKDWLSEEELHQLFRLRRVSKSQDLYYWERDELRPAFLSDNYYSR
                     EDLGQTREEFEKALDNADAVESWCDSWANPFDQNPIPKLAKSLEQSNQKNTSKIPFFT
                     AVDNNEKSSVIDEQYSKYFSNCSK"
     gene            complement(963..1181)
                     /locus_tag="AC06IO_002839"
                     /pseudo
     CDS             complement(963..1181)
                     /locus_tag="AC06IO_002839"
                     /inference="COORDINATES: protein motif:HMM:NF024999.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1547..2185
                     /locus_tag="AC06IO_002840"
     CDS             1547..2185
                     /locus_tag="AC06IO_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HIO9875997.1"
                     /translation="MKSSEICPFGPKLQQYWDKRYDYFHRFDEGIQTDSEGLHTVMPE
                     EAALTQAKLFNNAKIVVDGFCGIGGSAIALARTGKKVIAIEMDSVRLAMAKNNARVYG
                     VEHLITFVHGDFFDVAAEIKADAVLIDPPWGWPRYRKIEEFRLQHFNPNGSKLLSYVL
                     DHFNKIVLRAPKTFITSELDQFNVNYEIHSDKLHHSIISISVCITNHSKRKS"
BASE COUNT          753 a          420 c          446 g          716 t
ORIGIN      
        1 gaaaataacg gtttcgaaat tggaaatagt aaaataaata accacgtgat atattttttg
       61 tccttttaac caatttaagg tgaattaaat gttttctcca ctccaagatc cctggtctga
      121 attagaaaag cttggtttta acccagatta cctggaacgt cttaaaaagc gagctgctac
      181 ttctaaagaa cttgccgtta atgtagaaat gttggcagac tattatgaac gatttaaagc
      241 ggcaggtttc actgatcgcg atttggcgag gattgtgagc ctggtcagtt tcacctctga
      301 taatcgtcta tcaaatattg gaagccgaat gatgttaacc ttggtttgcg gaggattaga
      361 cttattaaaa gctcctctta atttcaactc ccataacctg actaatatga tggttagttt
      421 aaatggcttg gaaaaattac gacaattagt gaaaattaat cagtcgcata tcatcccatt
      481 caaaaatcaa gcagaattca aagcggtttt tgagaaaaaa tgtaaaaaat atccaatgtt
      541 gagagtagtg tggtgttatt ctgacaagga agaattgtat ctggaaaata gaagacaaga
      601 tgcaatttat aaagattggc ttagcgaaga ggaactgcat caacttttca gattacgccg
      661 tgtgtctaaa tcacaagatc tttactattg ggaacgtgat gaattaagac cagcatttct
      721 tagcgataac tattattcca gagaagatct cgggcaaaca cgagaagagt tcgaaaaagc
      781 actagacaat gctgatgcag tggaatcctg gtgtgactcg tgggcaaatc cctttgatca
      841 aaacccaatc cctaaactgg caaagtcatt agaacaatct aatcaaaaaa atacgagtaa
      901 aatacctttt tttactgctg ttgataataa tgaaaaaagt agtgtgatcg atgagcaata
      961 ctctaagtac ttttcaaatt gtagtaaata agcgcttggc aagaatgagt cttgccgagc
     1021 aaggtcgagt gaaggctttt taagctgctc attaactgac tgatagcctc aatagatatg
     1081 tggtcatcca aagtggcttc ggtcagctta acagccatta actcaccata atcattaatt
     1141 accagatgca gcttgaagct aaaaaaacaa cccattgacg attttccctt tttgtaaccc
     1201 cttcaaagat tcgattagag gaagctcttt ttttcaagag catacgtcaa tagctgttgc
     1261 atccacaaaa taaatacctg ttctctactt gaattacgtt accaggcacg atagcctatc
     1321 aaaacttaat agttagctta atcagcaact ctcgatccct ccggtgctga tgtaataagt
     1381 ggctatgtgt cgagggatac ttaaatataa caatattgag gtatcctcta tgcagaacgt
     1441 caatcaaaca actctattcc atacaaacaa ggaaactcta aaattttcaa tcctcgatta
     1501 tgcaggcagg cttgctatat ggggtgcact ttgatacagg taaaaaatga agtcatctga
     1561 aatttgccca tttggcccta aattacaaca atattgggat aagcgatacg attattttca
     1621 tcgctttgat gaggggattc aaacagactc tgaaggtttg cataccgtta tgcctgaaga
     1681 ggctgcgctc actcaagcca aattattcaa taatgcgaag attgtagtag atggtttttg
     1741 tggtattggc gggtcggcta ttgctctggc aagaacaggt aaaaaagtca ttgctatcga
     1801 aatggattcc gtccgtttag caatggctaa aaataatgct cgtgtttatg gtgttgaaca
     1861 tcttataacg tttgttcatg gagatttttt cgatgttgct gctgagataa aagctgatgc
     1921 tgtccttatt gacccgccat ggggatggcc tcgttacaga aagatagaag aatttcgttt
     1981 gcagcatttt aatccgaatg gaagtaaatt actcagttat gtgcttgatc atttcaataa
     2041 aattgtatta cgtgcaccaa aaacatttat cacttcagaa ttagatcaat ttaatgtcaa
     2101 ctatgagatt cattctgata agcttcatca ttcaatcatt tctatttctg tatgcatcac
     2161 taaccattcc aaaaggaagt cttgatagtg tttttaaata ccagatgagt ccatatcatt
     2221 gcttgctgtc caacccaagt cccaataata ctcccttcat tattggggga ttggtgtgct
     2281 atacattgaa caatccagtt aagaaaattt agtccttttg gtttattttc catat
//
LOCUS       DBNCWK010000224         2296 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.225, whole genome shotgun sequence.
ACCESSION   DBNCWK010000224 DBNCWK010000000
VERSION     DBNCWK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.225"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            139..1080
                     /locus_tag="AC06IO_002841"
     CDS             139..1080
                     /locus_tag="AC06IO_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HIO9875998.1"
                     /translation="MILNITAAQFPDLTLNAIESSQNIYRSIDFNFGEDADTAINKAS
                     LEKFINQFKSIHSTHDKPIEGIITVGKMKNVSPDTVKLLLTTEDFVQMLDQKSFLKLI
                     VTSNEIANFVLDNPKLRAKLDGIEPLVDAQKFENSCTARAIMKILLERGLIEPSSYTP
                     SKEMEIYKDIWLEPGKVASPEKIASYFCKYNLNVIGVEIRELSKSVRNKYSKDMVITS
                     LYSLFKKEVPIRKKMTLTTLSEADFPEGINTLIIIKAGVLHTLLGKKHHGQFEVTDPW
                     FGDKKIYSGFMDFLEKERKNLGVFFEISQGSQEIFRP"
     gene            complement(1457..2221)
                     /locus_tag="AC06IO_002842"
     CDS             complement(1457..2221)
                     /locus_tag="AC06IO_002842"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIO9875999.1"
                     /translation="MFNQNLAIIIGGSSGMGLETAKKLSSNNMELLITGKDKQKLENA
                     AAEIQKLGAKVDILVLDLYDKEQVFQFMSSMNDETRRVKYLVNAAGYFKPITFLEHEE
                     EDYDRQVNLNKAFFFITQAVVKNMKKNGGGSIVNIGSMWANQAVKATPSSAYSMQKAG
                     LHSLTQHLAMELAEHNIRVNAVAPAVVATPIYKSFIDEERIEESLQDFNKFHPIGRIG
                     KATDIADAIEFLLSEKASWITGEIMNVDGGVMAGRN"
BASE COUNT          753 a          414 c          415 g          714 t
ORIGIN      
        1 tttaatgaaa ggagaacaga atcagaatag gcaaacacaa gatacaattg aatttttata
       61 tatcacaaag aattatttat atgcaaaata acctataatc aagttagtgt cagttttggg
      121 atgtgaagac aggttattat gattttgaat attacagcag cgcagttccc ggatttaacg
      181 cttaatgcta ttgaatccag tcaaaatatc tatcgaagta ttgattttaa ttttggggaa
      241 gatgcggata cggcaattaa caaagccagt ttggagaaat ttatcaatca atttaagagt
      301 attcatagta ctcatgataa acctatcgaa gggattatta ccgtgggaaa gatgaaaaat
      361 gtctcccctg atacagtgaa actacttctt actacagaag atttcgttca aatgcttgat
      421 caaaaaagtt tcttaaaatt aatagtaact tccaatgaaa ttgcaaattt tgtattggat
      481 aatcctaaac taagagcaaa actggacggt attgaacctt tggtcgatgc acaaaaattc
      541 gaaaattctt gtactgcaag agcaataatg aaaatacttc ttgaaagggg gcttattgaa
      601 ccaagcagct atacacctag caaagaaatg gaaatataca aagacatttg gctagaaccc
      661 ggtaaagtgg catcgcctga aaagatcgca tcctattttt gcaaatataa tttgaatgtc
      721 attggtgtcg aaattagaga gctatcaaaa tctgtacgaa ataaatattc aaaagatatg
      781 gttattactt cactctattc actttttaag aaagaggtgc caataagaaa aaaaatgaca
      841 ttgacaacct taagtgaggc agatttccct gaggggataa acactcttat tattataaaa
      901 gctggagtgc tccatacctt attaggcaag aaacaccatg ggcagtttga agtaactgat
      961 ccctggtttg gtgataaaaa aatatatagt ggattcatgg actttcttga aaaagagaga
     1021 aaaaatctgg gagtattttt tgaaatttcc caaggatccc aagagatttt tagaccgtaa
     1081 tggttatcaa tgtttgccaa aagcaaatgg ccaaattatt ccatgtagca atgaaaagcc
     1141 ttagtgcaag catcaatagc aaaagattga tttttatgga taacgtgagt tatggataag
     1201 acagccatta ttttgaatcg ttcgggctga ggaggcattt atgccgtctc gaagccttgt
     1261 atggaattta atgtttcgtg ccaaggcttc gagacgatgc ttacgcatct cctcagccca
     1321 aacggaccct ggatattgac tgcttcttat ataatcctta tccagagcct acatcagata
     1381 aatctgaaac agggcattaa aaatcaatct tttgctattg atgctcgcag gcataaatct
     1441 ggataaggtt ttctgattaa tttcttcctg ccattacgcc accatcaaca ttcataatct
     1501 cccctgtaat ccagcttgct ttttctgaca aaagaaattc gattgcatca gcgatatctg
     1561 ttgccttacc gattctgccg ataggatgaa atttattaaa atcctgtaac gactcctcga
     1621 ttctctcttc atcaataaat gatttataga taggagtggc taccactgcc ggcgccacgg
     1681 cgtttactct tatattgtgc tcagcaagct ccatagctaa atgttgggtt agtgaatgaa
     1741 gtccggcttt ttgcatcgaa taagcagagg agggggttgc tttaaccgcc tgattcgccc
     1801 acatcgaacc gatattgacg atactacccc ctccattttt tttcatgttt tttactacgg
     1861 cctgggtgat aaaaaagaac gccttattaa gattaacctg acggtcgtaa tcttcttcct
     1921 catgttctaa aaaagtgatg ggtttgaaat aacctgcggc atttacaaga tattttactc
     1981 ttcgagtttc atcattcata ctgctcataa attggaatac ctgctctttg tcataaagat
     2041 caagaactaa aatatctact tttgctccca gtttttgtat ttctgccgca gcattttcaa
     2101 gtttttgttt atcttttccg gtaattaaca gctccatatt gttacttgaa agtttttttg
     2161 cagtttcaag gcccatacca ctagagccgc caataataat agctaaattt tgattgaaca
     2221 tggattctcc tgattcatta aaatactgtc caatccagta tgaaagaact acaatattaa
     2281 aaaaagtaag cacttt
//
LOCUS       DBNCWK010000225         2294 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.226, whole genome shotgun sequence.
ACCESSION   DBNCWK010000225 DBNCWK010000000
VERSION     DBNCWK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.226"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            74..>2294
                     /locus_tag="AC06IO_002843"
     CDS             74..>2294
                     /locus_tag="AC06IO_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIO9876000.1"
                     /translation="MAKSKQVIELPKLKQIKQHAGYKKNIEIARSLWNKAQPIQDNHA
                     AKSYLSLTRGLPETTIQQCEMRALPPEGAKPNRLITPVIDARGEIIGVQTVDLNPDGT
                     KAKPKNDGKTAIVDRRYIDSFGKAALVKKGSDPSRLYIAEGIETAASIVGVLNGDYTV
                     MASLGITEMVHAIPVIKAQLAPGAQIVLLADNDSDKQAVENLKNAVNCFREAGLNLVV
                     VKPKILDQDWNDVLQDKGNQQLKEEFCSLASESLLEQFDYEEGEQETLATAQKTVATL
                     IELLTPIEIQTHESQFLQIALTTIKEFQIQLEKLKATADKLQFNKGISKLLSELKQVH
                     EALTKLNVTDEELPPLPITISEMSNPAIKTHQQNKKYFEAAYHELKNRIRANKPITEL
                     ENHGDSNVDEVTYNKLVKGLKEYVSNHSALGTIPATDTAGEREKIAAAPAKNHLDYAE
                     KTGLLLHLEAMYKREIDLENKAIKVLTEEDNKSYEKQVKAQLDTIKSVLESLESKIVS
                     HSEQWEQVRDPAIQKAKKCYLGDMEKGNWLTQLVTQSWINELSSFHSASEFTYELNTL
                     PPTKKVCFIEGEEQSVDTTLENLRNQIIKRSGLLNMIDAGDNSPEQQLLKKSIEYYAH
                     ALAISMQRSFLVKIPGTNNYQEFDGINDKGKIIERKENNGTGEDYLQSNFTQRKINEK
                     MKFFRAVKTHHLHLMSELPENPEQYKNSYIFLKESGVKELYYVKPDGKYEKVKINDFN
                     "
BASE COUNT          851 a          425 c          456 g          562 t
ORIGIN      
        1 ttctcgatat aatttgcgcc aaacaaacga ttagaactct agttcaagaa tatacaaacg
       61 actgaggtat caaatggcta aatccaaaca agtaatcgag cttccaaaat tgaaacagat
      121 taagcaacat gcgggttaca aaaaaaatat agaaatcgca aggagcttat ggaataaagc
      181 tcaacctata caagataacc atgcagcaaa aagctatctg tcgttaacca gaggtttacc
      241 cgaaacaaca atacaacaat gtgaaatgcg cgccctgccg cctgagggag cgaaacccaa
      301 tcgattgatt actcctgtta ttgatgcgcg tggagaaata atcggggtgc aaacggttga
      361 tcttaatcca gatggcacta aggcgaaacc taaaaatgat gggaaaacgg caatcgtaga
      421 caggagatac attgattcct ttggtaaagc ggctctggta aaaaaaggta gtgatccgtc
      481 tcgcctctat attgcggaag gcatagaaac agcagcctcc atagtggggg tccttaatgg
      541 tgattataca gtaatggctt cgcttggcat taccgaaatg gttcatgcca ttcctgtaat
      601 caaggctcaa ctagcgcctg gggctcaaat tgttttgctt gcggacaatg acagtgataa
      661 gcaagcggta gaaaatctca aaaatgctgt taattgcttt cgcgaagcag gccttaatct
      721 ggttgttgta aaaccaaaaa tactggatca ggattggaat gatgtcctgc aagataaagg
      781 caatcaacag cttaaagaag agttctgttc cttggcttcc gaatcattac ttgagcaatt
      841 tgattatgag gaaggcgaac aggaaacatt ggctaccgct caaaaaacgg tggctactct
      901 cattgaattg ctaacgccga tcgagattca aactcatgaa agtcaatttc tacaaattgc
      961 tttaactaca attaaagagt ttcaaattca actcgaaaaa ttaaaagcaa ctgcagataa
     1021 gcttcagttc aataaaggca ttagcaaact acttagcgag ttaaaacagg tgcacgaagc
     1081 gcttaccaag ctcaatgtta ctgatgaaga gttaccaccc ctgccaatta ctatttctga
     1141 aatgtccaac ccggctataa aaactcacca acagaataaa aaatattttg aagcagccta
     1201 ccatgaatta aaaaacagga taagagccaa taaaccaatc accgaattgg agaatcatgg
     1261 ggacagcaat gttgatgaag tcacctataa caagttggtt aaaggtttaa aagaatatgt
     1321 atcgaatcac tccgccttgg gtaccatacc agcaacagat accgcaggag agagagaaaa
     1381 aattgctgca gcacctgcaa aaaatcatct cgattatgct gaaaaaacgg gtttattact
     1441 ccatcttgaa gcaatgtaca aacgagaaat tgaccttgaa aataaggcaa taaaagtcct
     1501 gactgaggaa gataacaaaa gctatgaaaa acaggtcaaa gcgcaattag atactattaa
     1561 aagcgtttta gaatcacttg agtcgaaaat agtcagtcat tcagaacaat gggagcaggt
     1621 tagagatcca gctattcaga aagcgaaaaa atgctactta ggtgacatgg aaaaaggtaa
     1681 ctggctaact caattggtaa cacagtcctg gattaatgag ctatcctctt ttcattccgc
     1741 cagtgaattt acttatgagt taaatacttt acccccaaca aaaaaagtat gctttattga
     1801 aggtgaagaa caaagtgtcg acaccaccct ggaaaattta agaaatcaaa ttattaaacg
     1861 cagtggtttg ctaaacatga ttgatgcggg agacaactct ccagaacagc aattattgaa
     1921 aaaatcaatt gagtattatg ctcatgcgct ggccatttca atgcagcgct cctttttagt
     1981 taagattcct ggaactaata attatcagga attcgacggc atcaatgaca aaggaaagat
     2041 aatagaacgc aaagaaaata atggaacagg cgaggattat ttacaaagca attttacgca
     2101 aagaaaaatt aacgagaaaa tgaaattttt cagggcagta aaaacgcatc acctccattt
     2161 gatgtccgaa ttacctgaaa atccagaaca atacaaaaac agctatatat ttctcaaaga
     2221 gagcggtgtt aaggaattgt attatgttaa gcctgatgga aaatatgaaa aggtaaaaat
     2281 taatgatttc aatt
//
LOCUS       DBNCWK010000226         2260 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.227, whole genome shotgun sequence.
ACCESSION   DBNCWK010000226 DBNCWK010000000
VERSION     DBNCWK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.227"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..602
                     /locus_tag="AC06IO_002844"
     CDS             <1..602
                     /locus_tag="AC06IO_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9876001.1"
                     /translation="VFGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGLLLTPVALGV
                     FLVKPLSFSILRKLGYKKLLILNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIA
                     LQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSFGVAISALLVSLFTFQVSQHFVL
                     TVKIFHLTFFALGILTILSGLIFTSLKKEDGKELIESPT"
     gene            complement(740..1258)
                     /locus_tag="AC06IO_002845"
     CDS             complement(740..1258)
                     /locus_tag="AC06IO_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1453 family Dot/Icm T4SS effector"
                     /protein_id="HIO9876002.1"
                     /translation="MPRWFRRIVDEGERLLVEVTDELRDESEVHKAASQLHLRVEQVL
                     NTNRARLEKPVIEAAEGFLHDLGVILEEDSMLRFPHAPVNQYLLAEKCTDIIYNYKPS
                     LESAPGIWNQIKAHINNFIEIIFGLENVLPTTQTMFAKDVSFTRCKRNLSRLKESLES
                     DAPGLLSVLVSC"
     gene            complement(1385..2236)
                     /locus_tag="AC06IO_002846"
     CDS             complement(1385..2236)
                     /locus_tag="AC06IO_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIO9876003.1"
                     /translation="MTSYQERQVKIPGFTIALKIWNSENPNPVLCLHGKMDNAASFDL
                     LAPLLSDRQLVAVDYPGTGLSSHYPEGVVPHWKNDAFLMCHVIKALGWKSFDIIAHSL
                     GSFLATVITIAQPKQVKKLVFLDILGPTVHIIENSMAYLPLNIESYLISGKQPRTVFS
                     SLDAAIKDRMNIGNISYQAAQALVQRGTKQDNEGWVWTFDPRLRCISATIPHEDEIRA
                     MFRAIDVPVCLILANQGVSYPESVFKGRSQSIKNLTIHRVQGGHHVHMDDPVPVAEII
                     SQYLSSK"
BASE COUNT          672 a          452 c          391 g          745 t
ORIGIN      
        1 cggtatttgg aaatttatta actcgtttaa gtttcggggg aatccctttt ttattgccat
       61 tactcttcca aattatttta gaatactctc cacagttgtc aggattgcta ctaacgccag
      121 tagcattggg tgtgttcctg gttaagccat tatcattctc tatcttacga aaattaggct
      181 ataaaaaatt actcattcta aatacagttt tagtctgttt ttctttatgg tcctttgcca
      241 ccatcaatca aagtacttct gtcatctaca ttggtttttt aacatttctt tatggatttt
      301 tcattgcgtt acaatacaca gggatgaact ctcttgcgta cgcgaatatt gacgataatg
      361 atatgagtta tgcgacaagt attatgagca cagttcaaca gttatcccaa agttttggag
      421 tagcaatttc tgccctactg gtcagtttat tcacttttca ggtttcgcag cattttgttt
      481 taacagtcaa gatttttcat ctaacctttt ttgctttagg gatacttacg attctttcag
      541 gattgatatt taccagctta aaaaaagagg atggaaaaga gctgattgag tcacctacct
      601 aggttttatc gctgttggac aggcaccaag atgaccaaga caatgcatct ctatgcagta
      661 tctggtcatt caagaaaagt ttagggcgtg ttgacaattg ccctaatgtg tatggaaatt
      721 aattaaatcc aaagacgatt taacaactaa ccagcacaga caacaaacca ggtgcatcac
      781 tttcaagtga ctcctttaat ctggaaaggt ttcttttgca tctggtgaaa ctcacatctt
      841 ttgcaaacat agtctgcgtc gttggtaaaa cattctctaa accgaaaatt atttcaataa
      901 agttattaat atgagccttg atctgattcc aaatccctgg tgctgactct aaggacggtt
      961 tgtaattgta aataatatca gtgcatttct ctgcgagtag atattggttt actggtgcat
     1021 gaggaaatct caacatggaa tcttcttcca ggatgacccc tagatcatga agaaaacctt
     1081 cagctgcttc aataaccggt ttttcaagtc tagctcgatt ggtatttaaa acttgttcga
     1141 cacgtaaatg taactgactt gctgctttat gtacttcact ttcatcacgc aattcatcgg
     1201 ttacctcgac aagcagcctt tctccctcat ctactattct tctaaaccat ctaggcatat
     1261 taatgctcca ctattaaata gattggccaa ttatacatca taaaatcatt cattatcaat
     1321 catccctgca tcaacattta gagcaagagt ccatctatac ttgttattct ttaataaacc
     1381 agttttattt ggagcttaaa tattgagata taatttctgc aacagggact ggatcatcca
     1441 tgtgcacatg atgtccccct tgtaccctat ggatggtcaa attttttatt gattgggagc
     1501 gtcctttaaa aacagattcg ggataagata caccttggtt agcgagaatt aggcaaactg
     1561 gcacgtctat agcacgaaac atggctcgaa tttcatcttc atgaggtata gttgcgctaa
     1621 tgcagcgtaa ccggggatca aatgtccaaa cccagccctc gttatcttgt tttgtacccc
     1681 tttgaaccag ggcttgagcc gcttgatagc ttatattacc aatattcatg cggtccttga
     1741 tagcagcatc cagactgcta aatacagtcc ttggttgttt accggatatt aaatagcttt
     1801 caatatttag aggtaaataa gccatgctgt tctcaataat atgaaccgtt ggtcctaaaa
     1861 tatctaaaaa taccaatttc ttaacttgtt taggttgcgc gatggttata acagttgcca
     1921 ggaaagaacc taatgaatga gcaataatat caaaactctt ccatcccaat gccttaataa
     1981 catggcacat gagaaaagca tcatttttcc aatgaggaac aacaccttca ggatagtgac
     2041 ttgacagtcc agttccagga taatctactg caaccaattg cctgtctgat aataaaggtg
     2101 ctagcaaatc aaaactggct gcgttatcca ttttaccatg taaacacaac actggattgg
     2161 gattttctga attccaaatt ttaagagcaa tagtaaaacc cgggatttta acttgtcttt
     2221 cttggtaaga tgtcaagata tataccttct taaaactctt
//
LOCUS       DBNCWK010000227         2244 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.228, whole genome shotgun sequence.
ACCESSION   DBNCWK010000227 DBNCWK010000000
VERSION     DBNCWK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.228"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(2..718)
                     /locus_tag="AC06IO_002847"
     CDS             complement(2..718)
                     /locus_tag="AC06IO_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIO9876004.1"
                     /translation="MEFLRRNRVGLVIIAILTCLFLILIPKQLSKKSKIVSQTPFLIE
                     IKLPDPAYSSPISIEEALKKRRSIRQYKNQPITLQQASQLLWAAQGITSDTGLRTAPS
                     AGACYPLELYFIASNVSNLSPGVYHYEPTKHTLKQLKAEDISSQLAQAALGQQAVKSS
                     AASIVITAVFSRMTKKYGKIGRRFALMEAGHVAQNIYLQSVSLNLATVSIGAFDITQV
                     REFLGIKDMEPVYILPVGAK"
     gene            1053..2222
                     /locus_tag="AC06IO_002848"
     CDS             1053..2222
                     /locus_tag="AC06IO_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876005.1"
                     /translation="MVQRIRRYAKKIYEDKFRFILIILILLLIAPMLLISQPKTVEGK
                     GIEMHFFYTPSCPYCAKQKLFNKRLLKNYKVKIVSHDITDPIEKKLFIFTAKQHGIAQ
                     HNLGVPLTIVNGKTIIGFESQESTGRLIEQALEQGDTKQPVSQIKPVETQKIPILGEV
                     DITHYSLPTLAILLGLIDGFNPCAMWALVFLIGLVTGLHDARKLWLLVGTFVFSSGVL
                     YFLFMTAWLNAFLLIGYIRPVVILIGAFAIILGILQIRDFFTKDIACPIPTQQKQKVM
                     RRMKILVLSPLTITTITGIIILAFVVNLIEFVCSSFIPAIFTQALALHELQTWQYYFY
                     VLLYDIFFMLDDLIIFSFAVFAVNRISTQYARWCKLIGGIALFVLGIVLLTTSNS"
BASE COUNT          663 a          416 c          413 g          752 t
ORIGIN      
        1 ttcattttgc tcctaccggc agaatatata caggctccat gtctttaata cccagaaact
       61 ccctgacttg agtaatatcg aacgcaccta ttgatacagt agccaagttg agagacaccg
      121 attgaaggta aatattttga gcaacatgcc ctgcctccat taaagcaaaa cgcctcccta
      181 ttttaccgta ttttttggtc attcgagaaa aaacggctgt gatcacaata cttgcagcgc
      241 tcgattttac tgcctgctgc cctaatgcgg cttgagctaa ttgtgaagag atatcctctg
      301 cctttaattg ttttagtgta tgctttgttg gttcataatg ataaacacca ggtgataaat
      361 tgctaacatt acttgcaata aaataaagct ccaatggata gcacgctcct gctgaaggtg
      421 ctgttcgcaa acccgtatca gaagttattc cttgcgctgc ccaaagtagc tgagaagctt
      481 gctgaagagt tatgggctga tttttatact gcctgataga gcggcgtttt ttaagcgctt
      541 cttcaattga aatggggctt gagtaagctg ggtctggtaa ttttatttca atcagaaacg
      601 gagtttgtga gacaatttta gatttttttg acaattgttt tggaattaaa atcaaaaaaa
      661 gacaggtcaa gatcgctatt attacgagcc ccactctgtt gcggcgtaaa aattccattt
      721 actatccccc tggtaataac tcccaaaata agatatttat tttttgtacc gtttttctcc
      781 tggattttga tattcaatag aaccctttat tctgtatgct gtcaatgttg atttaaaaat
      841 gaacttttgg ggaaatcgct tcgttaccca aatttttaac tgcctgattt tgttgcttgg
      901 gattttcttt ttgataacaa tagaatgtga aattttacca aactcacttg atccgtgaag
      961 gtgtgacgta aggaatgcag ggagtttttg ctcaattctc ataaaagata ttgattggca
     1021 tcaaaactat ctttaagaat taaaggaaca taatggttca aagaattcgg cgctatgcaa
     1081 aaaaaatcta tgaggataag tttcgattta ttctaataat tctcattcta ttgctcatag
     1141 ctccgatgct attgatatcc cagcctaaaa cagtggaagg caaaggcata gaaatgcatt
     1201 ttttctacac accaagctgt ccatactgtg ccaagcaaaa actatttaat aaaagattgc
     1261 taaaaaatta taaggtgaag atcgtcagcc atgacataac ggaccctatc gaaaagaaat
     1321 tatttatctt taccgcgaaa caacacggta tcgcgcagca taatttgggt gttcctttaa
     1381 ccattgttaa cggcaaaacc attattggtt ttgaatcaca agaaagcacc gggcgtctaa
     1441 ttgaacaggc tttggaacaa ggtgatacca aacaacccgt ctctcagata aaacctgtcg
     1501 aaacacagaa aataccaatt ctgggagaag ttgatataac gcattattct ttacccacac
     1561 tcgcaatact tcttggtctt atagatggtt ttaatccatg tgcgatgtgg gcattagttt
     1621 tcctgatagg tttggtaaca gggttacacg atgctagaaa actatggcta ttagttggaa
     1681 catttgtgtt ttcctcaggg gttttatatt ttttattcat gactgcctgg ttaaatgcat
     1741 ttctactcat tgggtatata cgccccgttg tcattttaat tggtgcattt gcaatcattc
     1801 tcggaatttt gcagataagg gattttttta ccaaagacat cgcttgccct attccaaccc
     1861 aacaaaaaca aaaagtcatg cgacgcatga aaattttagt gctctctcca cttacaataa
     1921 caacaattac cggcattatc attttggctt ttgttgtaaa tttaatagaa tttgtttgct
     1981 catcatttat tcctgcaatt tttactcaag cactggcttt acatgaactg caaacttggc
     2041 aatattattt ttatgttttg ctgtatgata tattttttat gcttgatgat ttgattatct
     2101 tcagttttgc agtttttgct gtcaacagaa taagtacaca gtatgcaaga tggtgtaagc
     2161 tcatcggggg gattgcgtta tttgttttag gtattgtttt actgacaact tctaactcgt
     2221 aacctaatta tcaaaggatt ttgc
//
LOCUS       DBNCWK010000228         2205 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.229, whole genome shotgun sequence.
ACCESSION   DBNCWK010000228 DBNCWK010000000
VERSION     DBNCWK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.229"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..>2205
                     /locus_tag="AC06IO_002849"
     CDS             <1..>2205
                     /locus_tag="AC06IO_002849"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIO9876006.1"
                     /translation="LKRVPHFLVLIIILLLGLPYIGLNWGLDFSNLAITINDGKIAGS
                     QLIESQIRGYFRQTLLQWSGFSLAAVTVLLAFTQYRLSNDKIALIIGLAILFSGSIEA
                     LHTLVIDGLALQGPEKNNLDALIWVFSNSVSGLILITGLSLLVHAGANKPVRLVTFIL
                     LTTFLVLAAIAVIYYAAVIIQIPDMWLYDSYVSRPFELISVFIYLMLMIIVYPKIYAS
                     YPTILSDSVFYMSLTQVVLAIYIMLLSTTPYDSAFNIAYFLKVVVYFIPCTCFVINYV
                     FSYSAVLEGQKNLKIKQEELKYMASHDPLTNLFNRREFEDLLDKSIANAQRNNSSLAL
                     LLIDLDNFKAINDTFGHFHGDELLKQFANRLVLLVRKGDLLSRVGGDEFTLISLNIES
                     PSSARHLAERILNELNSPYTINGKLITVTVSIGISIFPDDGNTTEDLLRKADLAMYKA
                     KNSGKNTYQFYTEQLGYSQHRESELESHLRKALQNDEFELYYQPKFNLINMEIIGAEI
                     LLRWNNEALGGVSPNEFIPVAENTGLITELSQWVLQKSCIQIMHWASQYNKMLSFSIN
                     ISPVQLTNPQFPKILEKILQEYQYPAEYLELEITENTLMGDQDEISKMLNVISDLGIK
                     LSLDDFGKEYSSLNRLKTLPIDSLKIDKTFISDIHNEHEKVVIIDIIIKLANELGMSI
                     VAEGIETIPQLKYLVSRKCHFGQGFLLSKPLTANEFTSIAYTKLGKTKSNAKV"
BASE COUNT          697 a          401 c          373 g          734 t
ORIGIN      
        1 ctaaaacgtg taccccattt tttggtattg attattattt tactgttagg tctgccttac
       61 ataggtctaa actggggttt agactttagt aatttagcca ttacaataaa tgatgggaaa
      121 atagccggct cccaactcat tgaatctcaa attcgcggtt acttccgaca aactctctta
      181 caatggtctg gattttctct tgctgcagtg acggtattat tagcatttac tcaatatcga
      241 ttaagtaatg ataaaattgc tttaattatt ggccttgcca ttctattctc tggctccatt
      301 gaagcgctgc ataccttggt tattgatggc ctggctttgc aaggtcccga aaaaaataac
      361 ctggacgctt taatttgggt tttttcaaac agtgttagcg gcttaattct aatcaccggg
      421 ctttctttac tggtgcatgc aggcgctaat aaaccagtga gattagtgac ttttattcta
      481 ctgactactt ttttggtctt ggctgctatc gcagtcattt attatgccgc tgtaataata
      541 caaattcctg atatgtggct ttatgattct tatgtttcca gaccttttga actcatatcc
      601 gtctttatct atcttatgtt gatgattatt gtttacccaa aaatctatgc atcttacccg
      661 actattttat ctgattctgt attctatatg tcactcaccc aagtcgtgtt ggctatctac
      721 atcatgttat tatcaacaac tccgtatgac agtgccttta atatagcata ttttcttaaa
      781 gtggttgttt attttatacc ttgcacctgt tttgtaatta attacgtttt ttcatactca
      841 gcggtactgg aagggcaaaa aaacttaaaa ataaaacagg aagaattaaa atatatggcc
      901 tctcatgatc ctctgacaaa cctgtttaat cgcagggaat ttgaagattt gcttgataaa
      961 tccattgcta atgcccaaag aaataactct tccctggctt tactgcttat tgatttagat
     1021 aatttcaagg caataaatga cacatttggt cattttcatg gcgatgaact tttaaaacaa
     1081 tttgccaatc gcctggtttt actagttcga aaaggtgatc ttttatccag agtgggtgga
     1141 gatgaattta cattaatctc tctgaatata gaatcccctt ccagcgcacg tcatctggca
     1201 gaacgaattt taaatgaatt aaacagtccc tatactatca atggcaaatt aatcacagta
     1261 acggtcagta taggtatatc tatttttcct gatgatggaa atactacaga agatctgtta
     1321 agaaaagcgg acttggctat gtataaagca aaaaactcag ggaaaaatac ctatcaattt
     1381 tatactgaac aactaggtta ttcacagcat agggaatccg agctggaatc tcatcttcga
     1441 aaagcattac aaaatgatga atttgaactt tattatcaac ccaaatttaa cctgattaat
     1501 atggagatta ttggcgcaga aattctttta cgctggaata atgaagccct gggaggggtg
     1561 tctcctaatg agtttatacc agtagcagaa aatactggat taataacaga actgagtcaa
     1621 tgggttttac aaaaatcgtg catacaaata atgcactggg ctagtcaata caacaagatg
     1681 ctttcctttt ccataaatat ttcgcctgtt caattaacaa atccacaatt tcctaaaatt
     1741 cttgaaaaaa tactacagga atatcaatac cctgcagaat atctcgaact tgaaattact
     1801 gaaaacactt taatggggga tcaagatgaa atctccaaaa tgcttaatgt aatttctgac
     1861 ttgggaataa agttgtcatt ggatgatttt ggcaaggaat actcttctct taacagatta
     1921 aaaactctcc ctattgacag cttaaaaatt gataaaacat ttatctctga tatacacaac
     1981 gaacatgaga aagtagtcat tatagacatt attattaaat tggctaatga gttaggaatg
     2041 agtattgttg ccgagggtat tgaaaccata cctcaattaa aatatcttgt gtcaagaaaa
     2101 tgtcattttg gtcaaggctt cctcctgagt aagcccttaa ctgcaaatga gttcacttca
     2161 attgcttata ccaagttggg aaaaaccaaa tctaacgcaa aagtc
//
LOCUS       DBNCWK010000229         2183 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.230, whole genome shotgun sequence.
ACCESSION   DBNCWK010000229 DBNCWK010000000
VERSION     DBNCWK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.230"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..685
                     /locus_tag="AC06IO_002850"
     CDS             <1..685
                     /locus_tag="AC06IO_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIO9876007.1"
                     /translation="SLYYIYPYIHGKRLFAEPEEIISLATALAKLHLKKKSYPDQQLI
                     IKNTTERTSQLNFIRKALADGCYSYIPHFSFVKKMAQQYDFDWINQEDAQPIHGDLNA
                     GNLLLSEDNMICFFDFEDALHSFHPVVLDLLFVIERIIFNQNSSTEQLLNLGLMFIHA
                     YKKAGGTYQYKTRDEFGLTILALKAFCLLTLLAEKNKNILSSEWDKFLKLTQKAENER
                     GLIKTILQG"
     gene            696..1823
                     /locus_tag="AC06IO_002851"
     CDS             696..1823
                     /locus_tag="AC06IO_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876008.1"
                     /translation="MRILVTARDVGAALNIIEIVKILKQYAGVTVFIYAQPPASKYFL
                     RAGIQPVFEVPLPPSRSLNEASDLLSYANQLIDKLNPDIILSGLSSPGEAGIDEAIIA
                     ECPAHIKTFVLQDFWGEVNFFFEKLADCYLCIDHHAAEITKKRFNAKTEVIGSPRHAI
                     YQTLNIEEQRNYIREYFNITQSTKTIGLFGQALHGIPGYMETISQWLQACLQQEKTKL
                     VIFYRPHPRESEADKEETIRLLNASGLEWKILSTKLVEESLLACDLVCSFFSNCLYDA
                     AYLNYFSLQPTLIPVVLNFNSQIQNYTNQLSLYEASPYRKQELAFFCESAHTLANDIA
                     HLLSKECSYKYWLQSKITLENPSKSIINTLTSLFNRIHNIA"
     gene            1949..2083
                     /locus_tag="AC06IO_002852"
     CDS             1949..2083
                     /locus_tag="AC06IO_002852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876009.1"
                     /translation="MTDLSQINLTPKNLEEALETIGHLAKIIIELKEENARLRGTKSI
                     "
BASE COUNT          763 a          380 c          342 g          698 t
ORIGIN      
        1 ctcactctat tatatttatc cctatattca tggaaaacgc ctctttgctg aaccagagga
       61 aataataagc cttgcaactg cgttagcaaa attacatctt aaaaaaaaat cttatcctga
      121 ccaacaattg attattaaaa ataccacgga acgaacatca cagcttaatt ttataagaaa
      181 ggctttagct gatggatgtt attcttacat tccacatttc tcttttgtca aaaagatggc
      241 tcaacaatat gattttgatt ggattaatca agaagatgcc caaccaattc atggtgattt
      301 aaatgcagga aatctgttac tatccgaaga taatatgatt tgcttttttg actttgaaga
      361 cgcattacat agtttccatc cagtagtgtt agatttgcta ttcgtcattg agcgcattat
      421 ttttaatcaa aatagttcga ctgaacagct attaaacctt ggcctcatgt ttattcatgc
      481 atataaaaag gctggtggca cataccaata taaaacaaga gatgaatttg gattaacaat
      541 tcttgcttta aaagcatttt gtttgttgac tttgctagca gagaaaaata aaaatatact
      601 gagttcggaa tgggataaat ttttaaaatt aacccaaaaa gcagaaaatg aacggggttt
      661 gataaaaaca attttacaag gataagaaag atagtgtgag aattcttgtg actgcgcggg
      721 atgttggtgc tgcgctaaac attattgaga tagtaaaaat acttaagcaa tatgcaggtg
      781 ttactgtatt tatttatgct caaccccctg cttcaaaata ttttcttcgg gctgggattc
      841 aacctgtatt tgaagttcca ctaccaccaa gccgatcctt aaatgaagcc agtgacctac
      901 tatcctacgc caatcaatta attgataaat taaatcccga tatcatatta tctggattat
      961 catctcctgg cgaagctggc atagatgaag caataatagc tgagtgtcct gcgcatatca
     1021 aaacattcgt ccttcaagat ttttggggag aagttaattt tttctttgaa aaattagctg
     1081 attgctattt atgcatagat catcatgctg cagaaattac aaaaaaacga tttaatgcca
     1141 aaaccgaggt gataggctcc cctcgccatg ccatttatca aacattaaat attgaggaac
     1201 aacgcaacta tataagagaa tattttaaca ttactcaaag tacaaaaact attggtcttt
     1261 ttgggcaagc acttcatggt atacctggat atatggaaac catttcacaa tggttgcaag
     1321 cttgcttaca acaagaaaaa accaaattag ttatttttta tcgcccccat cccagagaat
     1381 cagaagctga caaagaagaa actataagat tacttaatgc atctgggtta gagtggaaaa
     1441 ttttgtcgac taaattagtt gaagaatcat tgttagcatg cgacttggtt tgtagttttt
     1501 tttctaattg cctttacgat gcagcctatt taaactactt ttctttacaa cctacactga
     1561 tccctgttgt tcttaatttc aactcacaga ttcaaaatta cacaaatcaa ttgagcttat
     1621 atgaagcttc tccatatcga aagcaagagt tagctttttt ttgtgaatct gctcatacac
     1681 tagctaatga catagctcat ttattatcaa aagaatgttc ttataaatat tggctgcaaa
     1741 gtaaaattac attagaaaat ccatctaaat ctataattaa tacattaacc agtcttttta
     1801 atcgcattca taatattgca taaagctaac agtaatcgct cactcccctt gtcatgtaag
     1861 tatataaaat tatgatattt ttacgacaag atgtttttat taccatcaaa aatcgctata
     1921 aggagtcaaa agataagact ctgattttat gactgattta tcccaaatca atttaactcc
     1981 gaagaatcta gaagaagcat tagaaacaat aggccattta gcaaaaataa tcattgagtt
     2041 aaaagaagaa aatgctcgat taagaggcac caaatcaata tgagtcgcag atattaaaaa
     2101 cagtagatta attgcatgtc tcttttttct tgtctgccgg acctttggaa taaaaaaatt
     2161 gtaacttcaa aagccatccc tta
//
LOCUS       DBNCWK010000230         2170 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.231, whole genome shotgun sequence.
ACCESSION   DBNCWK010000230 DBNCWK010000000
VERSION     DBNCWK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.231"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(165..701)
                     /locus_tag="AC06IO_002853"
     CDS             complement(165..701)
                     /locus_tag="AC06IO_002853"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIO9876010.1"
                     /translation="MNYSIRKFEDKYPELGERVYIDPQSTVIGNVTLGDDVSVWPMAV
                     IRGDVNYIQIGHSCSIQDGAVLHVTHDGPYTPGGRPLILGQGITVGHKALLHACTIDD
                     YCLIGMGSIILDSAHIQKHVMIAAGSIVPPGKILKSEYLYLGSPVQAVRKLTAKEIEQ
                     IEYSAGHYIRLKNKYLTY"
     gene            complement(708..2066)
                     /gene="gorA"
                     /locus_tag="AC06IO_002854"
     CDS             complement(708..2066)
                     /gene="gorA"
                     /locus_tag="AC06IO_002854"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIO9876011.1"
                     /translation="MKTKHFDLIVLGGGSGGIASAVRAAQYGAKVAVIEQNHLGGTCV
                     NLGCVPKKIMYNASSIAETLHKSPDYGFFLENNAKLDWKRLVNKRNAYIERLRENYAK
                     RFSQHKITLIQGKGIFHDQSSITIDHTIYQAEHIIIATGGEPALPAINGIKHAIDSDG
                     FFSLTKLPAKVAVIGSGYIGVELAGILNSLGSETHLLMRGTRPLSRFDHMIGDTLMEI
                     MQKQGICIHQNHKAQAIHLHSDGRKSILCQSGSIIGNIDVIISAVGRKPRTGNLNLDK
                     INVNMDDKGLILVDAFQNTSVKGVYAIGDVTNAPALTPVAIAAGRRLADRIFGNQPDA
                     CLNYDNICSVVFSHPPSGSVGLTEYEAIEKYGKNKIKIYQTRFIPMYDALSIDKTPTA
                     MKLVTLGKKEKIIGLHVVGYSADEMLQGFGVAIKMGACKKDFDNTIAIHPTSAEEFVT
                     MV"
BASE COUNT          663 a          436 c          373 g          698 t
ORIGIN      
        1 cacaagccgc ttttttttta taaaataaat tacaccgtct tttctacata aaatactcgt
       61 tacttaactg gctctttaag tcataagttg agtttaaatg cttgatttca ttttttaatt
      121 tatcaaaagt aacatgtgga taagaaaaat aagtagctag taacctaata tgtaagatat
      181 ttattcttta accttatata atgccctgca gaatattcaa tttgctctat ctcttttgca
      241 gttaattttc tgacggcttg aactgggctg cccaaataca aatattcact tttgagtatt
      301 tttcccggag gtacgatgct tcctgcagca atcatgacat gtttttgtat gtgcgctgag
      361 tctaaaatta tagatcccat gccaattaaa cagtaatcat cgatagtgca ggcgtgtaat
      421 agagctttgt gccctaccgt gattccttgc cctaaaatta atggcctacc gcctggagta
      481 taaggaccat catgagtaac atgcaacaca gcaccatctt gaatactgca ggaatggcct
      541 atttgaatgt aattaacatc accacggata acagccatag gccatacgga tacgtcatct
      601 cccagggtta cgttaccaat gacagtggat tgcggatcaa tataaactcg ttcacctaat
      661 tcaggatact tgtcttcaaa tttacgaatg gaatagttca tgtatattta caccatagtt
      721 acaaattctt cagcgctggt gggatgaatt gcaattgtat tatcgaagtc ctttttacat
      781 gcgcccattt ttatagcaac cccaaagcct tgtagcattt catcggcgct gtatccgacg
      841 acatgtaaac caataatctt ttccttttta cccaaggtaa ccaatttcat cgcagtaggg
      901 gttttatcaa tactcaaggc atcatacata gggataaaac gggtttgata tattttaatt
      961 ttattcttac catatttttc aatggcttca tactcggtta aaccgacaga accagatgga
     1021 ggatgagaga atactactga acaaatatta tcataattta agcaggcatc aggttgattc
     1081 ccgaaaattc ggtctgccaa acgccttcct gccgctattg ctacaggagt taatgcggga
     1141 gcattagtga catcgccaat agcataaaca cctttaaccg atgtattttg gaatgcatca
     1201 accaggatta atcctttgtc atccatgtta acgtttattt tatcaagatt taaattgcca
     1261 gtccgcggtt ttcgacctac agcagatata atgacatcaa tatttccaat aatagagcca
     1321 ctttgacaca aaatactttt tctgccgtcg ctatggagat ggatagcttg ggctttatgg
     1381 ttttggtgaa tacagattcc ttgtttttgc ataatttcca ttaaagtatc gccaatcata
     1441 tgatcaaagc gactcagtgg tcgtgtacct ctcattaaca aatgagtttc actaccaaga
     1501 gagttaagga ttcctgctaa ttcaacacct atgtaaccgc taccaataac agctacctta
     1561 gctggtaatt tggttaatga aaaaaaacca tcagaatcaa tagcatgctt aatgccatta
     1621 attgctggta gagctggctc accgccagtt gcaataataa tatgttctgc ttgataaata
     1681 gtatgatcta tcgtgataga actttgatcg tggaaaatgc ccttaccttg aatcagagta
     1741 attttgtgct gtgaaaatcg tttcgcataa ttttctctta aacgttcaat atacgcattg
     1801 cgtttattca ctagtcgctt ccaatctaac ttggcattat tttccaggaa aaaaccgtaa
     1861 tcaggagatt tatggagtgt ttctgcaatt gatgaagcat tgtacataat ttttttgggg
     1921 acacatccta aattcacgca ggtccctccc aagtggtttt gctcaataac cgccactttg
     1981 gctccatact gagcagcacg aacagcactg gctattccgc cacttcctcc tccaagaact
     2041 ataaggtcaa aatgttttgt tttcatagtc aattaatagg aaaataaaat aatagtagca
     2101 ggtcgtactt ggtctaccaa gggattaaac tgaattttat aaaaaaccaa aatatttttt
     2161 aatttcaacc
//
LOCUS       DBNCWK010000231         2142 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.232, whole genome shotgun sequence.
ACCESSION   DBNCWK010000231 DBNCWK010000000
VERSION     DBNCWK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.232"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            61..780
                     /locus_tag="AC06IO_002855"
     CDS             61..780
                     /locus_tag="AC06IO_002855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9876012.1"
                     /translation="MSTVAQYKNYYHYISDGWSKEPKEMFKFLAQKIKELPLKPGNKL
                     LDIGCATGELIHFLRHQFPQLHYTGIDVFDDLIEVCKELQPNVNFINASALDLPFEFE
                     NQFDIITVVSVISIFDTIDLDIFWDNLFKATKDNGVIYVLSPFNEYGVDCEIRHRKRI
                     NNIKGEWERGWNIFCKETIAEYLEGRCKNWFFHPFKFNLELNQREDVIRTWTMKTEFN
                     DRQLTNGMKLMVDHYLLEIHI"
     gene            793..1878
                     /locus_tag="AC06IO_002856"
     CDS             793..1878
                     /locus_tag="AC06IO_002856"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0070085 - glycosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIO9876013.1"
                     /translation="MSQPFTIGPFSIGENHPPIFLAEIGTFFNQDLGIAEQLLHKIIT
                     IRDKVPYQPLILKTEILNNPNICLPVDYEETYTSKSGEVRKENYRSLIERKILPLEHY
                     SMLFSLLKNANLPFIVSIYELDTIDFAIKQGACALKTSSSNIVHIPLIRHLAKTGLPL
                     IIDTGRSSLTDICKAVETAKNAGCEKIIIEHSPDGHPALPEAHNLRMLRTLNQCFSLP
                     VGLSDHYNGIEMLYMSIALGASILEKGVHLYPEDIDQDISHTMGIDQLAKTLETIYNC
                     WQALGQPMRDLETPIKGVLGSSQRTCLVAKQKLQPGSLISLDNTTFAFPCKGVPTQYW
                     DLVNGWELQNKVSIGEPIKWSDIKQKK"
     gene            1875..>2142
                     /locus_tag="AC06IO_002857"
     CDS             1875..>2142
                     /locus_tag="AC06IO_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIO9876014.1"
                     /translation="MIVRNPTWNSLTPPLPKFVSCNTVETDFLKEKLGEDGRVYIAQF
                     DLKSTSAFLFQRKSDQKKFFIKKVSSEHKRQYQQSEHLAQFIACP"
BASE COUNT          745 a          364 c          370 g          663 t
ORIGIN      
        1 tacaaataaa tcagtataat aaaggataga tttattatat aaaaacggag gttattattt
       61 atgtcaactg tcgcgcaata caagaactac tatcattata tttccgacgg ctggagcaaa
      121 gaacctaaag aaatgttcaa gtttcttgct caaaaaatta aagaattgcc attgaaacct
      181 ggaaacaaat tattagatat tggctgtgca acaggagaat taattcattt tttaagacat
      241 caatttccac agctgcacta taccggaata gatgtttttg atgacttgat tgaggtatgc
      301 aaagaacttc aaccgaatgt aaattttatt aatgcatcag ccttggatct gccttttgaa
      361 tttgaaaatc aatttgatat tattactgtt gtaagtgtta tttctatctt cgatactatc
      421 gacttagata tattctggga taatctcttt aaagcaacca aagataacgg agtaatctat
      481 gtcttgtcac catttaatga atatggggta gattgtgaaa tacgacatag aaaaagaata
      541 aataatatta aaggggaatg ggaacgcggt tggaatattt tttgcaaaga aacgatagca
      601 gaatatcttg agggtcgttg taaaaattgg ttttttcatc ctttcaaatt taatcttgaa
      661 ttgaatcagc gtgaagacgt aattagaacc tggacaatga aaaccgagtt taatgatagg
      721 caattaacta atggtatgaa attaatggta gatcattatt tattagaaat tcacatataa
      781 acgagacagt caatgagtca gccttttaca attggaccat tctctattgg agaaaatcat
      841 cctcctattt ttttagcaga aataggtact ttttttaatc aggatcttgg catagctgaa
      901 caattgctac acaaaattat aaccatcaga gataaagtac cgtatcaacc tcttatacta
      961 aaaacagaaa ttttaaataa cccaaacatt tgcctacctg ttgattatga agaaacctac
     1021 actagtaaat caggtgaggt tcgcaaggaa aactaccgct cattaattga gagaaaaata
     1081 ctgcctttag aacattacag tatgctattc tcactattaa agaatgccaa tttacccttc
     1141 atagtttcaa tttatgaatt ggacactatt gattttgcaa taaagcaagg ggcctgcgca
     1201 ctcaaaacct cttcatcaaa cattgtccat attcctttaa ttcgtcatct ggcaaaaaca
     1261 ggattaccgc tcattattga cactggaaga tcttccttaa ctgatatatg caaagcagtt
     1321 gaaacagcaa aaaatgccgg atgtgaaaaa attattattg aacatagtcc tgatggccat
     1381 cctgctttgc cagaagcaca taacttaaga atgttacgaa ctttaaacca atgtttttca
     1441 ttaccagttg gattatctga ccattataat ggaattgaaa tgttgtatat gtctattgca
     1501 ttgggagctt caatcctgga aaaaggggtt catctctatc cagaagatat tgatcaggac
     1561 atatcacata ctatggggat tgatcaatta gccaagactc ttgaaacaat ttacaattgt
     1621 tggcaagctt taggtcaacc aatgcgagat ctggaaaccc ctataaaagg agtacttggc
     1681 agctctcaaa gaacttgttt ggttgcaaag caaaagcttc aacccggctc tctaattagc
     1741 cttgataata caacctttgc ctttccttgt aagggagttc ctacccaata ctgggatctg
     1801 gtaaatggct gggaactaca aaacaaggta tctataggtg agcccattaa atggtcagat
     1861 attaagcaaa aaaaatgatt gttagaaacc ctacttggaa ctctcttacc cctccgcttc
     1921 ctaaatttgt ttcttgtaat acagttgaga cagatttcct gaaagaaaaa ttaggcgaag
     1981 atggtagggt ttatattgct caatttgatc taaaatcaac ttctgctttt ttatttcagc
     2041 gtaaaagtga tcaaaaaaaa ttttttatca agaaagtatc ctctgaacat aaaaggcagt
     2101 atcagcagag cgaacatttg gctcaattta tcgcttgccc ag
//
LOCUS       DBNCWK010000232         2134 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.233, whole genome shotgun sequence.
ACCESSION   DBNCWK010000232 DBNCWK010000000
VERSION     DBNCWK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.233"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..800)
                     /locus_tag="AC06IO_002858"
     CDS             complement(<1..800)
                     /locus_tag="AC06IO_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HIO9876015.1"
                     /translation="MRFNHFIAPLVLLPVCALSQPQNDKGDVFLSKIPISKNAFVKSY
                     SSESAEFKLLESNTRSIDYPTQIIRISGSLESQQLDCDQVHAEILEKVVAPFAPDKFT
                     YNTYISCSYNPDTNMATGFLINSYIDPLNDDAVELLETYLAKNNGSNLLGTPLTFEPA
                     KALIVALTISAGMKKNPNKPPFVEYKQDRSNFYFKSNYEMRNKLFTDIYQNFFTNDPN
                     KILPFLDKWIFVNASSVYEAILMDSNFVELQPERIFLMENGEQIFVSNL"
     gene            complement(1060..>2134)
                     /locus_tag="AC06IO_002859"
     CDS             complement(1060..>2134)
                     /locus_tag="AC06IO_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876016.1"
                     /translation="PKLLGQELPQDDILHKLLRSGFIGVGISAVMILLFISTVFFTAP
                     LWLTAITTGLFVGASTYLSGILYGVVNDIFATHANLPYFLLGHQSQQTSLLRTNDKVA
                     QGIAWGVAATFGPVIIATLIFTVAATITAFFVPIATFLLPIMMIAMPLIAVGAEFYAR
                     KKTREYLDTEGNFYWIGSNDYQRRGLNYMCPTNEERAAWYANSDRNLFGFTKVPLIGL
                     GALIGLVVLSGISMFLPPVLFISPVIAIAIPAAFSAVTCATLIAGGIYMQVNRNKQLD
                     DRYRLEFERDEIVPDLYLDEDMEYIRKIAKIYAQNEPTVEPKQEVINTENYSFLFDKK
                     PKQAMPNIKQEEFEPARLSQAGA"
BASE COUNT          678 a          371 c          416 g          669 t
ORIGIN      
        1 agattggaaa caaatatttg ttcaccattt tccataagaa aaattcgttc aggttgcagt
       61 tcgacaaaat tggagtccat taaaatggct tcataaacgc ttgaggcatt aacgaaaatc
      121 cacttatcca ggaaaggtag aattttattg ggatcattgg taaagaaatt ttgataaata
      181 tcagtgaaca atttatttct catttcataa ttgcttttga agtagaagtt gctgcggtct
      241 tgcttatatt ctacgaaggg tggtttatta ggattttttt tcattcctgc agagatagtt
      301 aaagcaacga taagcgcttt agcgggctca aatgtcaatg gtgtacctaa cagattggaa
      361 ccattatttt ttgccaggta agtttctaat aattcaacgg catcatcatt tagtgggtct
      421 atataactat tgatgagaaa gccagtggcc atattggtat ccggattata actacaacta
      481 atataagtat tgtaagtaaa tttatcagga gcaaaaggag ccaccacttt ttcaagaatt
      541 tctgcgtgta cttgatcgca gtccagctgt tgtgattcga gagaaccaga gatacgaata
      601 atttgagtag gatagtcaat gcttctggta ttagattcaa gtaacttgaa ttctgccgat
      661 tcgctggaat aagattttac aaaagcattt ttactgattg gaatttttga taaaaagaca
      721 tctcctttgt cattttgggg ttggcttaat gcacaaacag ggagtaaaac cagtggagca
      781 ataaaatgat tgaatctcat agcaaactcc ttttttaaat taaatccttt aatttaaaga
      841 accagtttag cagggaacag attagatcag gttttgatca caagtgatcg catgaaaaca
      901 ggttctaatt ttgagtatac aggaatgatg caaattggct acatcatgga tatgcaaagt
      961 ttcttggaat taattcgggc agcgctcttt tggcaataat agagcgctac taattgattg
     1021 agtactaaac gtgtctttga atccgttcat ataggactat taggctccag cctgactcag
     1081 tcgtgccggt tcaaattctt cctgttttat atttggcatt gcttgttttg gttttttatc
     1141 aaaaaggaaa ctataatttt cagtgttgat aacttcttgt ttgggctcaa cggttggttc
     1201 attttgagca tatatttttg caatttttct aatatattcc atgtcttcgt ccagatataa
     1261 atcaggtaca atttcatcac gttcaaactc gagacggtaa cgatcatcca attgtttatt
     1321 acgattgact tgcatataga taccgccagc gattaaagta gcgcaggtaa cagcagaaaa
     1381 ggctgctgga atcgctattg ctataactgg cgagataaag agcactggtg gtagaaacat
     1441 gctaatacca cttagtacaa ctaacccaat cagcgccccc agaccaataa gaggtacctt
     1501 ggtaaaacca aataaatttc tatcggagtt tgcataccat gctgctcgct cttcattagt
     1561 cgggcacatg tagtttaaac ctcttctctg atagtcatta gaccctatcc aataaaaatt
     1621 tccctcggta tccagatatt cgcgtgtttt tttgcgagca taaaattctg cgccaactgc
     1681 aattaatggc attgcaatca tcattattgg taataaaaag gtagctattg gaacaaaaaa
     1741 agccgtaatt gttgctgcaa ctgtaaatat cagggttgcg ataattactg gaccaaaagt
     1801 tgcagcgacg ccccatgcta ttccttgcgc tactttatcg ttagttctta acagactggt
     1861 ttgttgagac tggtggccta acaggaaata gggcaagtta gcatgtgttg caaaaatatc
     1921 atttactacg ccataaagaa ttccacttaa ataggtagag gcacctacaa ataaaccggt
     1981 tgtgatagct gttaaccata agggggcagt aaaaaaaaca gtggagataa acaatagaat
     2041 cattacggct gaaataccaa caccaataaa tccagagcgc aataatttat gtaatatatc
     2101 gtcctgtggc aattcttgac ctaataattt agga
//
LOCUS       DBNCWK010000233         2128 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.234, whole genome shotgun sequence.
ACCESSION   DBNCWK010000233 DBNCWK010000000
VERSION     DBNCWK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.234"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            161..1762
                     /locus_tag="AC06IO_002860"
     CDS             161..1762
                     /locus_tag="AC06IO_002860"
                     /inference="COORDINATES: protein motif:HMM:NF012451.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIO9876017.1"
                     /translation="MNEFRYAIASYDSDKKGWVLFKVEYPDKSALEHALNTGKEVDLS
                     YLTFNNIEFSIASLFATTPFKSLQAEEKEGKSNREEASIKKTLAEIERLENQINETHA
                     QFIKDNIENNEVFNFFKDKSYIFEAAMQNYDVAKVFMSSSEVMSLLSPKQAIAVLFKH
                     RANIGQQFSLFDADNPRFISKDNPTIIEGLKSDSFEAKRFINSRLGALLSPLEFAKIL
                     LTHQDNSSFFIQTLARHEYAFSEESLKDPQVKEIYQQLLIKATDPVNARAYKKYFIQH
                     PAHIQLLFNFLMATENANAAIAFMNSYENMSLFSVKQVTELLLNHRAHLKLQNVPPFT
                     PHNPQLIALDNPMLINALKNDPGIAIQFINSRLINLFTPLTAAKIMLAHKDNDRFYHQ
                     TRPRHASLFTSENLNDPRIKEIYDQYFHNRNQRKNRFFAQQPQVNEAEEYFRFANRDE
                     IKFNPYKVLGVNEDASLEEIKKAYKKLAIAWHPDRVQAGENIEARTERFKKLGVAYNI
                     LIDPAKRQEYDNRPQNRFGRHNPMQ"
BASE COUNT          740 a          366 c          346 g          676 t
ORIGIN      
        1 atttgactat tattgttaat ttcaatagaa ttgaccccta ttttatcatc atgacctacc
       61 aatgagcata ttttatattt tttcaaatca actcactgat atcaacaatc ctcaaattca
      121 aaacattaaa aaaggtagaa tatttgattc tgagcataag atgaatgagt ttaggtacgc
      181 tattgcttct tatgattcag ataaaaaagg ttgggtgcta tttaaagtgg agtatcctga
      241 taaatctgct ctggagcatg cattaaacac aggaaaagag gtggatttaa gttatttaac
      301 ttttaataac attgaattct caatagcatc cttgtttgct acaactccgt ttaaaagcct
      361 ccaagctgaa gaaaaagaag ggaaatcaaa tagagaagag gcgagtatta aaaaaacact
      421 ggctgaaatt gaacgtttag aaaatcaaat taatgaaacc catgcgcaat tcattaaaga
      481 taatatagaa aataatgagg tttttaattt ttttaaagat aagtcctata tttttgaagc
      541 ggcaatgcaa aactatgatg tcgctaaagt attcatgagt tccagcgaag taatgtcttt
      601 attatctcct aagcaagcta tagctgtgct tttcaagcac agggcaaata taggccaaca
      661 attttcttta ttcgatgcag ataacccacg ctttatttct aaagataacc ccactattat
      721 tgaggggcta aaaagtgatt cttttgaagc caaaagattt ataaactcta gacttggtgc
      781 cttattaagc cctttagaat ttgctaaaat cttattaact caccaagata atagcagttt
      841 tttcattcaa actttagctc ggcatgagta tgcttttagt gaagaaagtt taaaagatcc
      901 tcaagttaaa gagatctatc agcaactatt aatcaaagct actgatccag ttaacgcaag
      961 agcttacaaa aaatatttta ttcaacatcc tgctcatatt cagttattat ttaatttctt
     1021 aatggccact gaaaatgcaa atgcagcaat tgcatttatg aattcttatg aaaacatgtc
     1081 attattttct gtcaagcaag tcacagaatt gcttttaaat catagagcac atctaaagct
     1141 acaaaacgta cctccattta cccctcataa tcctcaactt attgctttag ataatcctat
     1201 gcttattaac gcgttaaaaa acgatcctgg catagctatc caatttataa attcgagatt
     1261 gataaattta tttacacctt tgactgctgc aaaaattatg cttgcccata aagataatga
     1321 tcgtttctat catcaaacgc ggccacgcca tgcttcactt tttacttctg aaaacctgaa
     1381 tgatcccagg attaaagaaa tttatgatca atattttcat aatcgaaatc agagaaaaaa
     1441 tcgatttttt gcacaacaac cccaagttaa cgaggctgag gagtatttca gatttgcgaa
     1501 tagagatgaa ataaaattta atccctataa agtattaggc gttaacgaag atgcaagctt
     1561 ggaggagata aaaaaagcct ataaaaagtt agcaatagct tggcatcctg atagagtaca
     1621 agcaggtgaa aatatagaag cacgaacgga acgttttaag aaacttgggg tagcctataa
     1681 tattttaatt gatcctgcca aacgacagga atatgacaat agacctcaaa acagattcgg
     1741 aaggcataat cccatgcaat aagtgctttg ttattgggtt agcaatagca actgtcttga
     1801 attttattca agacagttgc ctcgggtgct gcaaatactg acacacctgg cagatttttg
     1861 atcttaagta atcaattcat atagcccgtg ttatccatct tttatgccct cctgcctact
     1921 tatgcactat agtattaaca ttcagattaa tttaatcatc ttgataatca gcatgtttca
     1981 aaaattttta ttacttgctt ttatgcttta tccatttgag tattcgttgg ccagcatacc
     2041 aaaagatgtg attgaatcct gtaaaaaggg ctcagcggtt agtggctcga ttgtagttac
     2101 ggatctcgga aatataggtt ttatcaga
//
LOCUS       DBNCWK010000234         2103 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.235, whole genome shotgun sequence.
ACCESSION   DBNCWK010000234 DBNCWK010000000
VERSION     DBNCWK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.235"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1389
                     /gene="lem29"
                     /locus_tag="AC06IO_002861"
     CDS             <1..1389
                     /gene="lem29"
                     /locus_tag="AC06IO_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem29"
                     /protein_id="HIO9876018.1"
                     /translation="YNQSFPNHYRVKVLRFCELICLEYKVGLEKKIKLSQRSLELLKD
                     YLNLGGAGPSNLVNGSSSIDFSSLTQLLASNLSKSSKTSKEIKKKREFIEAALYAELL
                     RDLSTSLNGSDEEIKPAEKEPPVSNKLKFWLLAIAGTLLAACEGFDSITTMMAVLPFP
                     AWVVLCAGILFSALSVVVFYGFDLVQVSQNLEIKLTDTPKLLDLYLSQMEEIKAIRKK
                     IDNYSLASMSVDELNELHLILVMLQDRVNSLVKAGKKFDLALNSPKMDIAKTIVSGVS
                     GILFFGGGFFAGQSVATFMLALVMTSVTPTFWPVLLFSVIVGLAAFSLYWYVQRVGVN
                     KLVSEWFGLDEDKIEKLCGKSKMDKELKKLNNLDEKVMTTSDLLSKVANLESKLIDVG
                     KQMKEMSTHQVPEKKLEGATGLKASSNVYSFHFNSSNRDQESVYGERGLDDAVSDYGR
                     GQNHNDSIHSLC"
     gene            complement(1274..>2103)
                     /locus_tag="AC06IO_002862"
     CDS             complement(1274..>2103)
                     /locus_tag="AC06IO_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876019.1"
                     /translation="AKKCFMERAMNRHDVKAIHSYVVDEEKKRISLKLINKLYRFLSK
                     YYHLASQEEFIRFIIQPDLYGELTVLYGENKEIAGFSRNCRQSVDLGKKQVTCYIAYL
                     YLNPEYQIHASIKSAGLTQAIKYKLANPHEELVYIAFANNPLTYEFIYQLSDLIYPKP
                     LQRVPEQILTVLNALKKQNGWISTNNHPMVVNSLLVPLRSQLSRINEESSELKEFYMS
                     ANPDYLQGNSLLIYMPLHLANIGYGLNHYDSDPYHNQKQHHQGPSHRKRSPDPDLRN"
BASE COUNT          615 a          342 c          450 g          696 t
ORIGIN      
        1 tacaatcaat ctttccctaa tcattaccgg gttaaggttc tcaggttctg tgagttaatt
       61 tgcttggagt ataaagtggg gcttgaaaaa aaaattaagc taagccagcg ttctctggaa
      121 ttattaaaag attacttaaa tttgggtggg gctgggcctt ccaatttagt gaatgggagt
      181 tcgtcgattg atttttcatc cttaactcaa ctacttgcca gtaatctttc caaatcatcc
      241 aaaacttcaa aagaaataaa aaagaaaaga gaatttatag aggcagcttt gtatgctgag
      301 ttgttacgtg acttaagtac ttcattgaat ggatcggatg aggaaatcaa gcctgcagaa
      361 aaagaacctc ctgtctctaa taagttgaaa ttctggcttt tggcaatagc tgggacattg
      421 ttagccgcat gcgaggggtt tgacagcatt acaaccatga tggctgtatt gccttttcct
      481 gcctgggttg tcctttgtgc aggtatttta ttttccgctt tatctgtcgt tgtattttat
      541 gggtttgatt tagtccaggt ttctcaaaac ctggaaataa aattaactga tactcccaag
      601 ctattggatt tatacctgtc acaaatggaa gaaattaagg cgattagaaa aaaaattgac
      661 aattattctc ttgcctctat gtcagtggat gagttgaatg agttgcattt aatccttgtt
      721 atgctgcaag accgtgtcaa ctctttagta aaagcaggca aaaaatttga tttggcactg
      781 aatagcccca aaatggatat tgctaaaaca attgtttctg gagtatccgg aatactcttt
      841 tttggtggag gtttttttgc cggacaatca gtagcgacat tcatgctggc tctggttatg
      901 acttctgtta cacctacatt ttggccagta ttattattta gtgtgattgt cggcttggct
      961 gcctttagtt tgtactggta tgtgcaacgc gtaggagtaa ataaactggt tagcgaatgg
     1021 tttggtttgg atgaggataa aatagaaaaa ttatgtggaa agtccaagat ggacaaagaa
     1081 ttgaaaaagc taaacaacct tgatgaaaaa gtaatgacta cttcggattt gctctcgaaa
     1141 gtggctaatt tggagagtaa actaatagat gtcggtaaac aaatgaagga gatgagtacg
     1201 caccaggttc ctgaaaagaa attagagggt gctactggat taaaggcaag ttctaatgtg
     1261 tattcctttc actttaattc ctcaaatcgg gatcaggaga gcgtttacgg tgagaggggc
     1321 cttgatgatg ctgtttctga ttatggtagg ggtcagaatc ataatgattc aatccatagc
     1381 ctatgttagc caaatgcaaa ggcatataga ttaacagcga gtttccttgc aaataatccg
     1441 gattggctga catataaaac tcttttaatt cactgctctc ttcattaatt cttgagagct
     1501 gacttcttaa aggaaccaat agggaattga caaccatagg atgattattg gttgaaatcc
     1561 agccgttttg ttttttaagg gcgtttagaa cggtcaatat ttgttctgga accctttgta
     1621 atggtttagg atatattagg tcactcaatt gataaataaa ttcgtaagtt aacgggttgt
     1681 tggcaaatgc tatgtaaacc agttcctcgt gaggattggc taacttgtat tttatcgcct
     1741 gagttaggcc ggcactttta atagatgcgt gaatttggta ttcaggattt agatacaaat
     1801 aagcgatata acaagtcact tgtttttttc ccagatcaac agattgtctg cagttgcggc
     1861 tgaatccggc tatttcttta ttttctccat agagaaccgt taactcacca tacaaatcag
     1921 gctgtatgat gaaacgtata aattcttctt ggctggccag gtgataatac ttggagagga
     1981 agcgatataa tttatttatt aattttaagg agattctttt cttttcttca tctacaacat
     2041 aagagtggat cgctttaaca tcgtgtctgt tcattgctct ctccatgaaa cattttttcg
     2101 ctt
//
LOCUS       DBNCWK010000235         2057 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.236, whole genome shotgun sequence.
ACCESSION   DBNCWK010000235 DBNCWK010000000
VERSION     DBNCWK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.236"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            40..762
                     /locus_tag="AC06IO_002863"
     CDS             40..762
                     /locus_tag="AC06IO_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HIO9876020.1"
                     /translation="MNYQYSPSLKKKLLLALTTLFIVQMFYFASVRASTRDTQVWTLM
                     NAIGPINKQHRKIRYWLEIQDRVGENISQLSQLILRPGIGYELLPTTTIWLGYDRIYT
                     AQPFANPPVNENRVWQQLLWSKEYTRLRLTARSRLEERFIQRATHTAWRYRQLFKTSI
                     FIPKHPKYAAVISDEAFFHLNDFNLQRNQGFDQNRLFVGLGYRTSKNSTIEIGYLNQM
                     IKRVNNPDYRGDYLSMALLLTE"
     gene            complement(821..1177)
                     /locus_tag="AC06IO_002864"
     CDS             complement(821..1177)
                     /locus_tag="AC06IO_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876021.1"
                     /translation="MSKFFTLLICISIGLLSFPTYAQKTIRLSPNETKTLKNSTLWTL
                     NATCNVQSSTQVNGKIKIIVLKNNGKVNGKSLSTGQGTSITIKNNSSISVSAESGTQI
                     NLINLGNQELQAVCST"
     gene            complement(1305..>2057)
                     /locus_tag="AC06IO_002865"
     CDS             complement(1305..>2057)
                     /locus_tag="AC06IO_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876022.1"
                     /translation="HSISTNKMRLVISMWLIYLFAVLFLVASLFTMLILMMEYTLVCK
                     NKQYHLAKECVLHSKIYNLYHSSTELGDLKAAVVKSSRSSKGNTLYYVDLLTAQGPVN
                     LTGGSSSGRDDKEIAANSINNYISNSLETTFKVSYPASWWMYGLSVFFLLVGAVLLTV
                     RGAITDFDRILKTIVIKRKGLFKTEETKLSFSDVDKIIVEESLGSRGTKTYRLAIALK
                     DKPPIPLVTTYDSSFAKKEKIANQLNQFIHES"
BASE COUNT          643 a          403 c          344 g          667 t
ORIGIN      
        1 aaacctgatg tgcagcaact ttttcagtct ttttaaacaa tgaattatca atattccccc
       61 agcctaaaga aaaaactcct gctcgcattg actactttat ttattgtaca aatgttctat
      121 tttgcctctg ttagagcatc aacaagagat actcaagtat ggacacttat gaatgcgata
      181 ggaccaatca ataagcagca tcgcaaaata agatattggc ttgagataca agatagagtt
      241 ggtgaaaata tttcgcaatt gtcccaactg atattacgcc ctggcatagg atatgaactc
      301 ttgcccacaa caaccatctg gctcggatat gatagaattt atactgcgca accatttgcc
      361 aaccctcctg taaacgaaaa tcgagtttgg caacaattat tgtggagcaa ggaatatact
      421 cgcttaagac ttactgcccg ttcgcgtttg gaagagcgct ttatccaaag agctactcat
      481 accgcctggc gatatagaca attatttaaa accagtattt ttattcccaa acaccctaaa
      541 tacgcagctg tgattagtga tgaagctttt tttcatctga atgacttcaa tcttcaacgt
      601 aatcaaggct ttgatcaaaa ccgtttattt gttggcttag gttatagaac cagtaaaaac
      661 agcactatag agattgggta tctcaatcaa atgatcaaac gcgtaaataa ccctgactat
      721 cgcggagatt atttatccat ggctctattg ttaacagaat aaacaagcgt acaaaacaaa
      781 ccattacttt caatggtttg tttgagaaaa attgatttaa ttaagtagaa caaactgctt
      841 gcaattcttg attacccaag ttaatcagat taatttgagt ccctgattct gcactaaccg
      901 agatactgct gttatttttt attgtaatag aagttccctg gccggttgaa aggcttttac
      961 catttacttt gccattgttc tttaaaacaa tgatttttat tttaccatta acctgggtgc
     1021 tgctctgtac gttacaggtc gcattcaaag tccataaagt gctatttttt agcgtcttgg
     1081 tttcgttagg gctaagtcgt atcgtttttt gagcataagt tgggaagctt aataaaccaa
     1141 ttgagataca tatcaacaaa gtaaaaaatt tagacatgct actttccttc tccagaatcc
     1201 aaagctcgac tattttaatg caatatgtat acattatatg cagatttact taagatttta
     1261 ttaagcccgt ttataaattt tttttgaagt atcccattca gcaatcagct ctcatggata
     1321 aattgattta attggtttgc aattttttct tttttagcaa aagaagaatc atacgttgtt
     1381 actaaaggaa taggtggctt atcctttaga gcgatggcaa gtctataagt tttcgtacct
     1441 ctgctcccaa gtgactcttc gactattatt ttatctacat cagaaaacga taatttggtt
     1501 tcctctgtct taaacaatcc ctttcttttg attacaattg tttttaatat tctgtcaaaa
     1561 tcagtaattg ctcctctaac ggtaagcaac acagcaccca ccaatagaaa aaaaacggat
     1621 aaaccataca tccaccacga ggctggataa ctcactttaa aagtcgtttc caagctatta
     1681 cttatgtagt tattgatgct gttggcagca atttctttat catcacgccc gctggaactg
     1741 ccccccgtca gattgactgg accttgagcc gttaacaaat ccacataata aagcgtattt
     1801 cctttacttg agcgactgct tttaactacg gctgctttta aatcacctaa ctcggtgctg
     1861 gagtgatata aattataaat tttgctgtgt aaaacacatt ccttagccaa atggtattgt
     1921 ttgtttttgc aaacgagggt gtattccatc attaaaataa gcattgtaaa aagacttgcc
     1981 acaagaaaca aaaccgcgaa cagatatata agccacatgc tgatgactag gcgcatcttg
     2041 tttgtgctga tagagtg
//
LOCUS       DBNCWK010000236         2028 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.237, whole genome shotgun sequence.
ACCESSION   DBNCWK010000236 DBNCWK010000000
VERSION     DBNCWK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.237"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..259
                     /locus_tag="AC06IO_002866"
     CDS             <1..259
                     /locus_tag="AC06IO_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIO9876023.1"
                     /translation="IIKKSVILKEAEGKINPRQEKVLLRLFQAGPDGFVGGLSAKNYM
                     SITGAPIATTTRDLNDLVKKNILKRTGELKATRYFLNLDKA"
     gene            355..798
                     /locus_tag="AC06IO_002867"
     CDS             355..798
                     /locus_tag="AC06IO_002867"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9876024.1"
                     /translation="MFFDDTYLDYKARVSYSLFCAKDFNETAAFIKANRILMTGTELA
                     AYMLKPENYKYAIIARIKNSIVGIVLFTDFESEVFINHLCVSPVHRFKGIGSNLLKEL
                     KAFMGQRTVSYIDTSNNYMPWMEVLDVSKKAAVINQLGSFYKRSR"
     gene            1353..2021
                     /locus_tag="AC06IO_002868"
     CDS             1353..2021
                     /locus_tag="AC06IO_002868"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIO9876025.1"
                     /translation="MIDTIIFDFDGVILNSEPMHFEALVQVLNQSGINLAYEEYMTHY
                     LGLSDISVFPKILNDKGLAFSSTEIRQLIERKVTVYNELIESSEQLPMTPDLDWFLVR
                     AAKQYGKIGICSGSNRHSIIKILEKIHCGRLACYFKTIVSCEDVSLGKPSPEGYLLAA
                     HHLQSNPENCLVIEDSEHGVAAAKAGGMLVAGLLTTLSREQLANADMIVHDFKELAHL
                     LNSH"
BASE COUNT          649 a          310 c          406 g          663 t
ORIGIN      
        1 cattattaag aaatctgtta ttttaaagga ggcagaagga aaaattaatc caaggcaaga
       61 aaaggtttta ttaagattat ttcaagcggg gcctgatgga tttgtaggtg gtttaagcgc
      121 taaaaactat atgagtataa caggcgctcc catcgctaca acaaccagag atttaaatga
      181 cttggttaaa aaaaatattc tcaaaagaac cggtgagctt aaagcgacgc gttatttttt
      241 aaatcttgat aaggcttaaa tggagtttgt gagatttggt acgcagtgtg tactgtacat
      301 ttcgcaggtt ttgtatttaa atgcacgcca tttagatcgt taatgttgca atttatgttt
      361 tttgatgaca cttatttgga ttataaagca agagttagtt actctttgtt ttgcgctaaa
      421 gattttaatg aaacagctgc ttttatcaaa gctaatcgaa ttttaatgac tggtaccgag
      481 ttagccgctt atatgctaaa accagaaaat tataaatatg ccattattgc cagaataaag
      541 aatagcattg tgggcattgt tttattcacg gattttgaat cggaagtatt cattaatcat
      601 ctttgtgtaa gtcctgtaca tcgttttaaa gggatcggct caaatttatt gaaagaatta
      661 aaagctttta tgggacaaag aacagtctct tatatagata ctagtaataa ctatatgcct
      721 tggatggagg ttttggatgt ttccaagaaa gctgcagtta ttaatcaatt aggttcattt
      781 tataaacgta gtaggtgaca gccaaagtag tcttactgtc ccttataaaa acctattcaa
      841 ctttatcatc aagagattta attgtttcta aattcatttg tataatgttt tggatggcta
      901 ctccaatttc aactaaattt cttgcttcga ttgaagtttt cttttgtgag ttttctatgc
      961 ttttcttagt tcatgaagca aagagtaaaa ggccatgaaa ttattatgag gcatattgtc
     1021 gacccggatt tataaatggt cacaaatagt acatgaaaat gtagtgtgtc aggacggcat
     1081 agggattggt tcatgaaaat ggtttgggtg ttaataaaaa ataatagtct cttgttacta
     1141 tttcaagctt tttactttta aaaaacagga aataagtagt ttaaaaattc ctatacctct
     1201 tttgattaag cagaccaata caagcaattt taagagtagt tgtctaaaat aaaatgaaat
     1261 atccctttta tcaaacgccc agcatgaaag taatttctct tgagtaattg tcaatttatt
     1321 tgtttaacaa tgttaaccta ctgaggcaat acatgataga tacaataatc tttgattttg
     1381 atggtgtaat acttaacagt gaacccatgc attttgaagc gttagtccag gtactgaatc
     1441 aatctgggat taatcttgct tatgaagaat atatgaccca ttacctggga ttaagcgata
     1501 ttagtgtgtt tcccaaaata ctgaatgata agggtcttgc gttctcatcc actgaaatac
     1561 gtcaacttat agagcgcaag gtcacagttt ataatgagtt aatcgagagc agtgagcaat
     1621 tgcctatgac ccctgatctg gattggtttt tagtccgagc ggcgaaacaa tatggaaaaa
     1681 tcggcatctg cagcggatcc aatcgccatt caataataaa aattcttgaa aaaattcatt
     1741 gcggtcgctt ggcttgctat tttaaaacca ttgtaagttg tgaagatgta agcttgggta
     1801 aaccgtctcc agaaggttat ttgctcgcag cgcatcattt gcaatccaat cctgaaaact
     1861 gtttggttat agaggactca gagcacgggg tagcagccgc gaaagcggga ggaatgctgg
     1921 ttgcgggttt actcacaaca ctgagcaggg agcagctggc caatgcggat atgattgtgc
     1981 atgattttaa agagctggct catttgttga actcccacta aaaagaga
//
LOCUS       DBNCWK010000237         1879 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.238, whole genome shotgun sequence.
ACCESSION   DBNCWK010000237 DBNCWK010000000
VERSION     DBNCWK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.238"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(209..1285)
                     /locus_tag="AC06IO_002869"
     CDS             complement(209..1285)
                     /locus_tag="AC06IO_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIO9876026.1"
                     /translation="MITIRNKIGQMLIMGFHGSELHDNSPVAQWLSNDGLGGVLLFDK
                     DLSTGIYGKNLRNQAQIRQLIRQLNRYASMISGGNDNLPLFTAIDYEGGVVDRLSKIE
                     GCPPTMKARELAKLSSDDFDEEVTQMAMTLQSLGFNLNFAPVVDLNLNAHAGIIGNLG
                     RSFSSNPEVTIRLAKQFVDVFSRYGIACAYKHFPGHGSATGDTHEGFVDVTETFQTDE
                     LIPYRSLLKDLSNPVMVMTAHVINKHLDSKGLPATLSYDVLTLLLRQSIGYDGVIISD
                     DLQMHAISNHYSLEEALCLTINAGADMVIFANQLGTITAPEVIDVIEKLVIDKQISSQ
                     RIDEAYRRIVRLKQQIICSELINT"
     gene            complement(1413..1859)
                     /locus_tag="AC06IO_002870"
     CDS             complement(1413..1859)
                     /locus_tag="AC06IO_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876027.1"
                     /translation="MYLRLLALSALCFVTSPIWSFTCIYTLVKDNCWTDYDVTVDIIE
                     DSTSKTLLTVTAPKGKSWARGTFNCEAAEGLRYVAQFSPVFWQNDVGKTYPALRNWYL
                     PAKVNPGDLAWTIPVCFPADFAQVPFPPNVAGNCKCNFKNIPDPKL"
BASE COUNT          600 a          408 c          329 g          542 t
ORIGIN      
        1 tttttcttct agtcaatagg ataaataaac tgtgtcaatt atattcttca cagaacacac
       61 catatagata ctaataaaat acttcaaacc caatctcaaa caattatgtc tgttcatgct
      121 gaaaagaaga tggagcctct ctaataggtg aagttatggt ggtaacttga tggttcataa
      181 atccgccagc taccacttgg catgggcatt acgtattgat caattcactg catataattt
      241 gttgcttcag acgtactatt cttctgtatg cctcatcaat acgttgagag gaaatttgct
      301 tgtcaattac cagtttttca atcacatcta taacttccgg cgcggtaata gttccaagct
      361 gattggcaaa tatgaccata tcagccccgg cattgatggt aagacaaaga gcctcttcta
      421 atgaatagtg atttgaaatg gcatgcattt gcaaatcatc gctgataatg accccgtcat
      481 atcctatgct ttgacgtaac aataacgtta aaacgtcgta ggacagagtt gctggcaatc
      541 ccttactatc caaatgctta ttaatcacat gtgccgtcat taccatgaca gggttagaca
      601 ggtcttttaa taaacttcga taaggaatta gctcatcggt ttgaaaggtt tctgtaacat
      661 ctacaaatcc ctcatgtgta tctccagtgg cgctgccatg gccggggaaa tgtttatacg
      721 cacaagcaat accataacga ctgaaaacat caacgaactg cttggccaaa cgaatagtaa
      781 cctctgggtt agaagaaaag cttcttccca agtttccaat aatccctgca tgagcattta
      841 aatttaaatc aacaaccggg gcaaaattta aattaaaacc taatgactgc aacgtcatag
      901 ccatttgagt tacttcttca tcaaaatcat cactggataa ctttgcaagt tccctggcct
      961 tcatagtagg cggacagccc tctattttgc ttagcctgtc aaccactccg ccttcgtaat
     1021 caatcgcagt aaataaaggc agattgtcat tacctcctga aatcattgat gcataacgat
     1081 taagctgtcg aatcaattgc cttatctgag cctgattcct taaattcttg ccataaatcc
     1141 ctgtggatag atctttgtcg aataacaaga ctccgccgag accatcatta gataaccatt
     1201 gtgcaaccgg actattatca tgcagctcgg aaccatgaaa tcccataatg agcatttgtc
     1261 cgattttgtt tcttattgta atcaatcact cgctccatca caaaataagc ataaaatcat
     1321 acacaaagat attctctctg gctatgcccc gctacgcatt atcaataaac agtatggcaa
     1381 tgtgtacaaa taaaaacatg cttctcataa aattaaagct tgggatcagg aatgttcttg
     1441 aagttacatt tacagtttcc tgctacatta ggtggaaagg gaacttgagc aaaatctgcc
     1501 ggaaaacaaa cggggatagt ccaggccaaa tctccaggat tcacttttgc tggtaaatac
     1561 caattcctta atgccgggta ggtttttccg acatcatttt gccaaaagac aggcgaaaat
     1621 tgagcgacat atctcaaccc ttcagcagcc tcacaattga aagtacctct agcccatgat
     1681 tttcctttgg gagcggtaac tgtcaacaaa gtttttgacg tagaatcttc aatgatatcg
     1741 acagtaacat cataatcagt ccaacaattg tctttaacca aggtatatat acatgtgaaa
     1801 gaccaaattg ggctagtcac aaagcacaat gcagataggg cgagtaaacg taagtacatg
     1861 attcaatcct aagatttaa
//
LOCUS       DBNCWK010000238         1839 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.239, whole genome shotgun sequence.
ACCESSION   DBNCWK010000238 DBNCWK010000000
VERSION     DBNCWK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.239"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            68..>1839
                     /locus_tag="AC06IO_002871"
     CDS             68..>1839
                     /locus_tag="AC06IO_002871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876028.1"
                     /translation="MTRNKTEKNIQFDFNECLEKAKANELSSLDILNNKDTWDSLSIK
                     QKQRLCGEWVRHGYFSFEEFNDPDCAPELYGLLNEDIYIVGIKQDPVRLQNIPDDILS
                     TILPVLYEDRNFMDDLIDKACEDITILQSSQELHKKVIELAAHQIWGGKKSIFIRRDT
                     RFYKDVEEFIIESINQGKFVINNENTFLSGQFHYSSGFLAHSPAICMAILRKNPGKLF
                     IVSKDILPLILNDSETMGVIVQALRCGEIEMKTIPTSMKNKDICFAALIFNLRNNIFV
                     PEEIREQLDYEEIYYEAMLANPAALKDIPDDMKSKIINRFSHEEFFQLIQKYHFNCLP
                     YLPDEWVEKIKRQVLPKVRTVVVYDPARYDREIRMSCQLYASRPQYQDQTIVINSEDL
                     NHLLKTLSKVSEHSSIKLVINGHSSYRSEQLAGLSSQSIVHYLEKYPIIHDLVLSGCS
                     TVKMEVTAQEKEMIKEFIFLINQKSIENNRSPEEIIDELVANGVFEKGHPEYKEYKKF
                     VPFLQSITVTPEEIEKNLKESLMKLVIDELEKSKKISHSITIKAIPSPLNVNETYEKS
                     TRGFVVWNKQLYKEGKTGLDSDLR"
BASE COUNT          670 a          288 c          350 g          531 t
ORIGIN      
        1 taacgaaaat atcaccttga tgactttggg ttcatctcaa gagtagaaaa tattgggtgg
       61 ttaagcaatg accagaaata aaacagaaaa aaatattcaa tttgacttta atgaatgttt
      121 ggaaaaagcc aaagccaacg aattgtcttc tttggacatt ttaaataata aggacacgtg
      181 ggatagttta tctatcaaac aaaaacaacg gttatgtggg gagtgggtgc ggcatggata
      241 cttcagtttt gaagaattta acgacccgga ttgtgcacca gagctctatg gattgttgaa
      301 tgaagacata tatattgttg gcataaagca agatcctgtt cgtttacaga atattcctga
      361 cgacattcta tcaacaattt tacccgtctt atatgaagat agaaatttta tggatgatct
      421 tattgataaa gcttgtgaag atataacgat tctccaaagc tctcaagagc tgcataaaaa
      481 agtaatagag cttgcggcac atcagatatg gggagggaaa aaatctatat tcataaggag
      541 ggatactcga ttttataaag atgtcgagga atttatcata gaatctatca atcagggaaa
      601 atttgtaatt aataatgaaa atactttttt gtccggtcaa tttcactatt cttccggttt
      661 tctagcacat agtcctgcga tatgcatggc catactaaga aaaaaccctg gaaaattatt
      721 tattgtatcg aaagatatat tacccctaat acttaacgat agtgaaacga tgggggtcat
      781 cgtacaagct ctacgttgtg gggaaattga aatgaaaaca attcccacct ctatgaaaaa
      841 caaggatatt tgctttgcag cacttatatt caatcttaga aataatattt ttgttccaga
      901 ggaaatccga gaacagcttg attatgaaga gatttactat gaggcaatgt tagcaaatcc
      961 tgccgcctta aaagacattc ctgacgatat gaaaagtaaa attatcaatc gattttctca
     1021 tgaggaattt tttcaactta ttcaaaaata ccattttaat tgtctaccct atttgcctga
     1081 tgaatgggta gaaaaaataa aaaggcaggt attgcctaaa gttaggaccg ttgtcgtcta
     1141 tgaccctgct cgttatgatc gggaaatacg aatgagttgt caattatatg caagcaggcc
     1201 tcaatatcaa gatcaaacga tcgttattaa tagcgaggat ttgaatcatt tattaaaaac
     1261 gttatctaaa gtatccgagc attccagtat aaaactggtg atcaatggtc acagcagtta
     1321 taggtcagag caattggctg gattgagtag tcaatccatc gtgcattatc tagaaaaata
     1381 tcccattatt catgatctcg tcctatcagg ttgtagcacg gtaaaaatgg aagtgactgc
     1441 tcaagagaaa gagatgatta aggaatttat tttccttata aaccaaaaga gtattgaaaa
     1501 taataggtca ccagaagaaa ttatcgatga gcttgtggcg aacggtgtat ttgaaaaagg
     1561 ccatccagaa tataaagaat ataaaaaatt tgtaccattc cttcagagca ttacagtaac
     1621 gccagaagaa atagaaaaaa atttaaaaga atcgttgatg aagctggtca ttgatgaact
     1681 tgaaaaatca aaaaaaatct ctcattccat tactataaaa gccattccta gcccacttaa
     1741 tgtaaatgaa acatacgaaa aaagtacccg aggctttgtc gtatggaata aacaactata
     1801 caaggaaggg aaaacagggc tcgattcaga tttaaggaa
//
LOCUS       DBNCWK010000239         1824 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.240, whole genome shotgun sequence.
ACCESSION   DBNCWK010000239 DBNCWK010000000
VERSION     DBNCWK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.240"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..890)
                     /locus_tag="AC06IO_002872"
     CDS             complement(<1..890)
                     /locus_tag="AC06IO_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIO9876029.1"
                     /translation="MAKILITGATGFIGRSLVPALLSEGHDVRCAVLQLDSTLQAEQI
                     VINNLEVHTDWTDALWNVEIVIHLAARVHIMKEYAISSLDEYCKINSIATKNFAEQAA
                     QNNVKRFIFLSTIKVHGEFSQNSLPVTEDCRTQPEDPYAKSKLYAEQFIQEICQNTRM
                     EFVILRPPLVYGPYVKANFLRILQLVDKKWPLPFGSIYNKRTFIYIDNLVSAISTVVS
                     EPSAANQIYLVADDSSWSLTQLVQTLSLKMNTKLFLIPVPVQILIFLFKLCGLKNINT
                     RLFSSLEVSNKKIKSQLGWTP"
     gene            complement(911..1786)
                     /gene="rfbA"
                     /locus_tag="AC06IO_002873"
     CDS             complement(911..1786)
                     /gene="rfbA"
                     /locus_tag="AC06IO_002873"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIO9876030.1"
                     /translation="MKGIILAGGFGTRLYPLTKSISKHMIPVYDKPMIYYAISTLMLA
                     NIREILIISTEEHLPLYQNLLSDGSQWGISFAYLVQNEPRGIAEAFILGEKFIGNDSV
                     CLVLGDNIHQGRGFSELLQNAKSKLNGATVFAYYVDKPQAYGVVEFNEKQQVLSIVEK
                     PAQPKSNYAVTGLYFYDNQVIEIAKSLKPSGRGELEITDINQFYLYENKLDVQILGRG
                     FVWLDMGTPETLLSASNYICTIEQRQGLKIGCPEEIAWRMKFISDDELLCRARELNKS
                     SYGQYLAELLNHKRY"
BASE COUNT          574 a          368 c          283 g          599 t
ORIGIN      
        1 ggtgtccacc ctaattgaga tttaattttc ttattactta cttctaaaga agaaaacaat
       61 ctggtattaa tatttttgag tccacataat ttaaatagaa aaattaaaat ttgaactgga
      121 accgggatta aaaataattt tgtattcatc ttgagcgata aggtttgcac tagttgtgtt
      181 aaagaccatg aactatcatc tgctaccaaa taaatttgat tagcggcact aggctcgctc
      241 acaactgtgc ttattgctga aaccaaatta tcaatatata taaaagttcg tttattataa
      301 atactaccaa aaggtaaagg ccatttttta tcaacaagtt gtaatatcct gagaaaatta
      361 gccttcacgt aaggtccata gaccaacggt ggtcttaata taacaaactc catccgggta
      421 ttctggcaaa tttcttgtat aaattgttca gcatacagct tactttttgc atagggatct
      481 tcaggttgag tcctacaatc ttcagtaact ggcagactat tttgtgaaaa ttcgccatgt
      541 acttttatgg tactaagaaa tataaatcgc tttacattat tttgagctgc ctgctcagca
      601 aaattttttg tagcaatact attaattttg caatattcgt ctaaagaaga tattgcatat
      661 tctttcatta tgtgaactct ggcagctaaa tgaatgacta tttccacatt ccacaaagca
      721 tcagtccaat cagtgtgaac ctcaagattg tttattacta tttgttctgc ttgtaatgta
      781 ctatccagct gcaaaacagc acaacgcaca tcatgtcctt cagaaagcaa ggcaggaact
      841 aaacttcgtc ctataaatcc tgtcgcgcca gtaattaata tttttgccac tactttataa
      901 cctgaaaaaa ttaatatcgt ttatgattaa gcagttctgc aagatattga ccgtaactac
      961 ttttattaag ctctctagcc ctgcacagta actcatcatc actaataaac ttcatacgcc
     1021 acgcgatttc ttcagggcat ccaattttta aaccttgccg ctgttcgata gtacagatat
     1081 agttacttgc tgataacagg gtctctggtg tacccatatc cagccagaca aaacctcttc
     1141 ccaaaatctg gacatccagt ttgttttcat ataaataaaa ctgattgatg tcagttattt
     1201 ccagctcccc ccttccagag ggttttaaac tcttggcaat ttctataacc tgattatcat
     1261 aaaaatataa tccagttact gcgtagttgg atttgggctg tgctggtttt tccactatgg
     1321 ataggacttg ttgtttttca ttgaactcca ccacaccata ggcctgaggt ttatcgacat
     1381 aataggcaaa aactgttgca ccgtttaatt ttgattttgc attttgtaat aattcagaaa
     1441 aacctctgcc ctggtggata ttatccccca aaaccaggca aacagaatca ttgccaataa
     1501 atttttcacc taaaatgaat gcttccgcga ttccccttgg ttcattttgc acaagatagg
     1561 caaatgagat gccccactga gaaccatcac tcaataaatt ttgatacagc ggaagatgct
     1621 cctccgttga aataatcagt atttccctga tattagccaa cattaatgta gagatggcat
     1681 aataaatcat tggtttatca taaaccggaa tcatatgctt actaatggat tttgtcagag
     1741 gatacagtct tgtaccaaaa ccacctgcca ggataatacc tttcattata tcgagcctac
     1801 tctctatttt tctttgaaca aata
//
LOCUS       DBNCWK010000240         1753 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.241, whole genome shotgun sequence.
ACCESSION   DBNCWK010000240 DBNCWK010000000
VERSION     DBNCWK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.241"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            2..904
                     /locus_tag="AC06IO_002874"
     CDS             2..904
                     /locus_tag="AC06IO_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIO9876031.1"
                     /translation="MADLQSFLAVVELHSISLAAKKLHITQPALSKRIRKLESEWNTQ
                     LFISSGLRTELTDKGKQLLPYVRQMVYLSHELISSTGTDKKKPQLLLNIGTTVYIAQT
                     IIPPLIIHMNALDLNYFINTKLIADKDVAHGLSEGAYDLIISPFMSNSAEIKSVPLWR
                     ERLIPIVGLSHPLAKIKEKLSFAELAEFDAVLMEKNFIIRRIIDKEAEKQKLTLKIRA
                     EANIIYNNIALVEQGMAWSMINERLLNPNLKPLELSDYALSIDFYCHFLQKRTDERLI
                     RIFIDNLVNWVNTSSELSSFSLVN"
     gene            complement(914..1147)
                     /locus_tag="AC06IO_002875"
     CDS             complement(914..1147)
                     /locus_tag="AC06IO_002875"
                     /inference="COORDINATES: protein motif:HMM:NF013842.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HIO9876032.1"
                     /translation="MLRHIDNGGTLEGVSRLFSVGTTTIKNWKRLRAQTGKLEGSGRP
                     CNPYKIDADALKLYLKERPDAFLNGNPPIFNWQ"
     gene            1236..1397
                     /locus_tag="AC06IO_002876"
                     /pseudo
     CDS             1236..1397
                     /locus_tag="AC06IO_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          563 a          321 c          300 g          569 t
ORIGIN      
        1 tattgccgat ttgcaatctt ttcttgcagt tgtagaactc cactctatct ctttagctgc
       61 caaaaaattg catattactc agcctgcttt aagtaaaaga attagaaaac ttgagtcgga
      121 gtggaatacc caacttttta tttcttcagg attaaggact gagctaactg ataaaggaaa
      181 acaattattg ccttatgttc ggcagatggt ttatctgagc catgagttaa ttagcagtac
      241 tggtacagat aaaaaaaaac ctcagttact gctaaatatt ggtaccacag tatatattgc
      301 tcaaactatt atcccccccc ttataatcca tatgaacgca ctggacttga actacttcat
      361 taatacgaaa ctgattgctg acaaagatgt agctcacggt ttaagcgaag gtgcttatga
      421 tctgattatc tctccgttca tgagtaattc tgctgaaatt aagtctgtac ctttgtggcg
      481 agaaaggctc atcccaattg tcgggttatc acatcctcta gcaaaaatta aagaaaaact
      541 atcctttgct gaattagcag aattcgatgc ggtgcttatg gaaaaaaact ttataatacg
      601 ccgaattatt gataaagaag ctgaaaaaca aaagttgact ctcaaaataa gagctgaagc
      661 caatattatt tataacaaca tagctcttgt cgaacaaggt atggcttgga gtatgattaa
      721 tgaacgctta ttgaatccta atttaaaacc tctcgaacta tctgattatg ccctcagcat
      781 tgatttttat tgtcattttc ttcagaaacg tacagatgaa cgattgattc gtatttttat
      841 tgataatctg gtcaattggg ttaacacgtc aagtgaatta agctcttttt cccttgttaa
      901 ttagataaac cctttattgc cagttaaaaa tggggggatt accgtttaga aaagcatcag
      961 gacgctcttt taggtataat tttaatgcat cagcatctat tttgtaggga ttgcaagggc
     1021 gacctgaacc ctctagcttc cctgtttgtg ctctcagacg cttccagttt tttattgtag
     1081 tggtccctac tgaaaacaat ctacttactc cttctaacgt gcctccgtta tctatatgcc
     1141 gtaacacgtt ttctcaaagt ctaatgaata ggtcataaat taaaaagcgt cataataaca
     1201 aattacttgg gcatttcaaa ttttaaatca gtatagttca ttggcgtaat tatcaaacca
     1261 gacgggaggc aaaacaagat attctggatt acattactat gttttataac aatcaaagat
     1321 tgcattcgtc tttggattat ctaagtccga atcaatttga aaatcggtac tgggggttaa
     1381 tgaaaaaagg ggcttaactg ggatgtgcaa attggattga ccacttcatg atttagtcga
     1441 ttaggagaat gctgcctgat ctatttccat catacataac ttttaactgt agctcatcga
     1501 ccattcatat tcccaataca tcctttcagt cacgatctaa agaataaaat tttgttccat
     1561 actttaggag gatacaacag taatagccta catcgatagc aaaaaccagc agcaactctc
     1621 ttttagtcat ttacgtttgc cgaaaacgtc acagtgcatt cagaatattt gattatggaa
     1681 attaaatgct tactcggtca gatactgatt ttagagaatc aattaaattt tagtcaatta
     1741 cagaattaat taa
//
LOCUS       DBNCWK010000241         1740 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.242, whole genome shotgun sequence.
ACCESSION   DBNCWK010000241 DBNCWK010000000
VERSION     DBNCWK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.242"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            59..1330
                     /gene="pcnB"
                     /locus_tag="AC06IO_002877"
     CDS             59..1330
                     /gene="pcnB"
                     /locus_tag="AC06IO_002877"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIO9876033.1"
                     /translation="MAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRD
                     LLLHKAPKDFDIATNATPNEVKNLFKNARIIGRRFKLVHILFHREIIEVATFRGHNSS
                     DASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKL
                     IRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDE
                     ITKLYQCGEAESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDG
                     KPVTPAFLIAVLLWFPMIECSRKLQESGLPPLPSIEKAMSIVLSEQNAIISIPKRYSQ
                     VIREIWLLQYRFDKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWATFQE
                     VDDVEKNNMVTQLAATTPGRPKKRRKRKVAK"
     gene            1327..>1740
                     /gene="folK"
                     /locus_tag="AC06IO_002878"
     CDS             1327..>1740
                     /gene="folK"
                     /locus_tag="AC06IO_002878"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIO9876034.1"
                     /translation="MNVCYLSLGSNQKNPERQIRQAIKEIKQIPSTCLIKVSRLYWNK
                     AWGFENQQEFCNVVIEIRTTLLPHKLLKWCQKIENRHQRVRRKFWGPRTLDIDIILYG
                     YRLIRTKNLIVPHPCFHLRDFVLIPLRELNSNLKIP"
BASE COUNT          550 a          323 c          339 g          528 t
ORIGIN      
        1 catcaagttt attaaaaaac tgttaagaag aaacaggtcg catggacatg caattgactt
       61 ggcttatatc atcccgagaa ataaacataa catatcgaaa gcagatataa gtaataatgc
      121 gcttaaagtt cttaaccgtt taatcagcca tggttttcag gcctatatcg ttggaggcag
      181 tgtcagagat ttactgctcc ataaagcgcc taaagatttt gatatcgcga caaatgcgac
      241 tcccaatgag gtaaaaaatc ttttcaaaaa tgctcgtatc attggtcgtc gatttaaact
      301 ggtacatatt ttatttcatc gtgaaattat tgaagtagca acttttcgtg gccataattc
      361 aagcgatgca agccaccaat ttaatgaacg cggcatgctt gtaagagata atgtttatgg
      421 ctctctggac gaagacgcat ggagacgtga tttcacaata aactctcttt attataatat
      481 ttctgattct tcaatcattg attttactgg tggtgtagaa gacgttaagc aaaagttaat
      541 tcgaataatt ggcgatcccg ttacgcgtta tcaagaggat cctgtccgta tgttacgtgc
      601 tgtcaggttt agtgccaagc tgcaatttaa tttatctccg gaaaccaaag ctcccttccc
      661 caaaataagc catcttataa cccatgtttc cagttcgcga ctatttgatg aaataacgaa
      721 actttatcaa tgcggagaag ccgaatcggt tcaaaaatta ttggttgagt atggtttatt
      781 taattatcta tttcctcaaa cagctgctct atttgatagt cattacccgg ttaatcctct
      841 tttgggcata gcacttgaaa atactgatac aagaatacgt gatggaaagc ctgttactcc
      901 ggcatttctt attgctgttt tgttatggtt tccaatgata gagtgttcca gaaaactgca
      961 agaatctggg cttccgcctt taccctcgat tgaaaaagcc atgtctatag tgcttagtga
     1021 acagaatgca attatttcga tacctaaaag gtatagccag gttataaggg aaatttggct
     1081 tttgcaatat cgcttcgaca aaagatttgg tggcagagca tttaatttac ttcttcatcc
     1141 ccgattcaga gcagcgtatg attttatggc tcttagggca ttatctggtg atgagtcaat
     1201 ggagcttgcc cagtggtggg ccacgtttca agaagtcgat gatgttgaaa aaaataacat
     1261 ggtaacccaa ttggctgcaa caacgcccgg gcggcctaaa aaacgtcgta aacggaaagt
     1321 agcaaaatga atgtttgcta tctgagcttg ggatcgaatc aaaaaaatcc tgagcgtcaa
     1381 ataagacaag ccattaaaga aatcaaacag attccatcaa catgccttat caaggtctct
     1441 cgtttatatt ggaataaggc ctggggcttt gaaaatcaac aggaattttg taatgttgtt
     1501 atagagatac gaactactct gttaccccat aagctcctta agtggtgcca gaaaatagaa
     1561 aacaggcacc agcgagtcag gagaaagttt tggggaccaa gaacattaga tattgatatc
     1621 attttatatg gttatagatt aattcgcaca aaaaacttaa tcgttccaca tccttgtttc
     1681 catttacgag attttgttct tattcctcta agagaattaa actctaattt aaaaatacca
//
LOCUS       DBNCWK010000242         1734 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.243, whole genome shotgun sequence.
ACCESSION   DBNCWK010000242 DBNCWK010000000
VERSION     DBNCWK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.243"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..992
                     /locus_tag="AC06IO_002879"
     CDS             <1..992
                     /locus_tag="AC06IO_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HIO9876035.1"
                     /translation="TRKMKNNKPVITVDLNDPVKCLENLEKAFQTGTFILKNSPVNFR
                     RLEKVLSNLDTFYNLDFKTKIDISGQISGGGATRGYFPLGVESGLVDVFEKKESYSFG
                     WPDNEIPDSDASNLSLVAPNIYPDHGYINPDDVNGLIKDFANTAKKLSRLIEIILFNG
                     KTVLPIDESSDFQSLLRSFRYYKKTEFDPPNTGANSLHTDWNLITLVYADADGYQYRD
                     NEGNFQNIIPETNDCLIVNFGDFLSAWTDGKIVSPWHQVVEAKKKDVRNSLVFFYYPA
                     ASSSAVKNPIYNQGEPLSLFVDQSDPLNRLWDFSKIRTMNEMYAEKWKQVSRR"
     gene            complement(1133..1582)
                     /locus_tag="AC06IO_002880"
     CDS             complement(1133..1582)
                     /locus_tag="AC06IO_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIO9876036.1"
                     /translation="MSLLLTSPSFEHNDFIPDEFTCHGLDRPPPLEWENAPPNTQSYV
                     LIMDDPDALGGLWVHWVLFNIPASYNELDAAIDVPDSAISVKNSWGKTGYGGPCPPSG
                     THRYYFRLYALDTFLSLDEKATKKDVEYAMQGHILETSELICKYTKI"
BASE COUNT          556 a          299 c          319 g          560 t
ORIGIN      
        1 taacgagaaa gatgaaaaat aataagcctg taatcactgt tgacttaaat gatccggtta
       61 agtgcttgga aaatcttgaa aaggctttcc agactggtac ttttattcta aaaaattcgc
      121 cggtgaattt cagaagatta gagaaggttt tatctaatct ggatacattt tacaatttgg
      181 attttaaaac caaaatagat atttccggac aaatttccgg cggtggtgca accagaggct
      241 attttcccct gggtgtagaa agtggtcttg ttgatgtgtt tgaaaaaaaa gaatcttatt
      301 cgtttgggtg gcctgataat gaaatcccgg attcagatgc ctcaaattta tcattagttg
      361 ctcccaatat ctatcctgat catggttata tcaacccaga tgatgttaat ggattgataa
      421 aagattttgc caatacagca aaaaaattat ccaggttaat tgaaatcatc ctcttcaatg
      481 gaaaaactgt tttaccgatt gatgaatctt cagattttca atcgttgtta cgaagcttca
      541 ggtattacaa aaagacagaa tttgatcctc ctaatacagg ggcaaattcg cttcacacag
      601 attggaacct gattactttg gtatatgcag atgcagatgg ataccaatat agagataatg
      661 agggcaactt tcaaaacata atccctgaaa cgaatgattg ccttatagtt aattttggag
      721 attttttatc agcttggact gatggaaaaa ttgttagtcc ttggcatcag gtagttgagg
      781 caaagaaaaa agatgtaaga aattctctgg tcttcttcta ttatccagca gcctctagct
      841 cagccgttaa aaatcctata tataatcaag gtgaacctct aagtttgttt gttgatcagt
      901 ctgatccttt aaacaggtta tgggactttt ctaagataag aactatgaat gaaatgtatg
      961 cagaaaaatg gaaacaagtg agtagaagat agggttctgt ttaagacatt aagcaatgat
     1021 tatcataaca aaaaataaaa aaacaccctt ggctggtata ttttttgttc cccaaaaaat
     1081 agggttatgc tgtatattag gaatcattgt tctcttctaa tctcaagagg gattagattt
     1141 tagtatattt acaaatgagc tcacttgtct caagaatatg gccttgcata gcatattcaa
     1201 cgtctttctt tgttgctttt tcatccagag ataaaaaagt atccaaagca tataacctaa
     1261 aataatatcg atgtgttccg cttggtggac atggtccgcc gtaacctgtt ttgccccaac
     1321 tattcttaac gcttatcgct gagtcgggta catctatagc tgcatccagt tcgttataac
     1381 ttgccggtat gttgaataaa acccagtgaa cccataatcc tcctaacgca tccggatcat
     1441 ccataattaa tacataggat tgggtatttg gcggggcgtt ttcccactca agcggtggag
     1501 ggcgatccaa tccatggcaa gtaaattcat caggaataaa atcgttatgt tcaaatgacg
     1561 ggcttgttag caataaactc aaaatactct ccttgtctat ctcggataca gcattgtgaa
     1621 tcttaatctt ttgttatttt tccttactac acttaaatta tattagcatg gtaattttgg
     1681 atctgccatt catcaaagtc atcaagaaat ttaaaatatt attctcttca ttga
//
LOCUS       DBNCWK010000243         1713 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.244, whole genome shotgun sequence.
ACCESSION   DBNCWK010000243 DBNCWK010000000
VERSION     DBNCWK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.244"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..196
                     /locus_tag="AC06IO_002881"
     CDS             <1..196
                     /locus_tag="AC06IO_002881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876037.1"
                     /translation="IAFLFGLSIVSLQGCAVAAVGAGIAAVKYGNARKAEVKQTCLKN
                     YNDYLRLTKNPVPLSKYCPD"
     gene            complement(340..657)
                     /locus_tag="AC06IO_002882"
     CDS             complement(340..657)
                     /locus_tag="AC06IO_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIO9876038.1"
                     /translation="MNLQTIKSLDGKIEYVLLPVATYNVLRQQSNEQLKHTQEDYEIF
                     NPADYVDNPVALARIQTGLTQEELAKKMEVTQAFISKIENQEKVTPKMLTKVKQALSN
                     RQD"
     gene            939..1220
                     /locus_tag="AC06IO_002883"
     CDS             939..1220
                     /locus_tag="AC06IO_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIO9876039.1"
                     /translation="MISDFSCKETKKIYDGTVSRKFPQDIQQRARRKLRMLNNAKVLD
                     DLRIPPSNHLEKLVGDRAGQHSIKINDQWRICFVWQDGIVTEVEIVDYH"
     gene            1230..1550
                     /locus_tag="AC06IO_002884"
     CDS             1230..1550
                     /locus_tag="AC06IO_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIO9876040.1"
                     /translation="MMKLQNIHPGEILLEEFLLPMGISQNKLANDIGVPPRRINEIVH
                     GKRSITADTDLRLSRALGTSEGFWLGLQADYDLEEKKASIKSKLEKIKSLISFEETIS
                     KYAS"
BASE COUNT          550 a          268 c          331 g          564 t
ORIGIN      
        1 gatagcattt ttatttggtt tgagtatagt tagtcttcaa ggatgcgctg ttgcagcagt
       61 gggtgcaggt atagctgcgg ttaagtacgg taatgcaaga aaagcagaag taaaacaaac
      121 atgcttaaaa aattataatg attatcttcg attaactaaa aatcctgtcc cgctttcaaa
      181 atactgtcct gattagtcat attgggtggc aaaggaactc attgatatac ttgctaactt
      241 gcacgaaatc aagtcttttg gtagacccgc tttgctttgt ccacccatag cctaaaagag
      301 ttctcggcat tagtctgtcg atacgggatt attggttcat taatcctgac gattggataa
      361 agcctgttta actttagtta acattttagg cgtaactttt tcttgattct caattttact
      421 aataaaagct tgtgtaactt ccattttctt agctaattct tcttgagtta atcctgtttg
      481 aatacgagcc aatgctactg ggttatcaac gtaatcagca ggattaaaga tctcataatc
      541 ttcttgggta tgttttagtt gctcattact ttgttgacgt aatacattat aagtggctac
      601 aggcaataaa acatattcga ttttgccatc aagagattta attgtttgta aattcattct
      661 gtcgaaaata agagtcgcct tttaatttct ttatatcgaa gattggatca tttgaatctt
      721 tacccagcaa gctcgttggc gagctgtaat acggaattat ttgatcatta atcatggcaa
      781 ttagttaaag ccagctaaac tttagttaac attttagtca taactttgat taaataacta
      841 agttatgcaa ttgtttgtta atcagaaata taacgttttg cgttatataa cgttttgcgt
      901 tatacttgct aataatatga ataaattggc atggcagagt gattagcgat tttagctgta
      961 aggaaacaaa aaaaatatat gatggcacgg tgtctcgaaa atttccgcaa gacattcaac
     1021 aacgtgctag aagaaagtta agaatgctaa ataatgccaa agttttagat gatttaagga
     1081 tacctccaag taatcatctt gagaaattag ttggtgatcg ggcagggcaa catagcataa
     1141 agataaacga tcagtggcgc atttgttttg tttggcaaga tggcatcgta actgaagttg
     1201 aaattgttga ctaccattag gagtatatga tgatgaagtt acaaaatatc catccaggtg
     1261 aaatcttatt agaggaattt ttgctaccaa tgggtatcag ccaaaataag cttgctaatg
     1321 atattggtgt tcctccaagg cgtattaatg aaattgttca tggtaagcgc tctatcacag
     1381 cagatactga tttaagatta tcccgtgcat taggaacgtc tgaaggcttt tggttgggat
     1441 tgcaagctga ttatgattta gaagaaaaga aagctagtat taaaagtaag ttagaaaaaa
     1501 ttaagtcttt gatttccttt gaagagacta ttagtaaata tgctagttaa caaataaaaa
     1561 cttgaattca tttagagcac tcagcacgtt cagggtcagg tcttgctttt tgccaacttc
     1621 tagtcagaag tcaaaaagca gagcctgctc ccagatatac tctcaattca gaatctggaa
     1681 atgccaggct gttaacccta tttaaaaaca agc
//
LOCUS       DBNCWK010000244         1711 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.245, whole genome shotgun sequence.
ACCESSION   DBNCWK010000244 DBNCWK010000000
VERSION     DBNCWK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.245"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..938
                     /locus_tag="AC06IO_002885"
     CDS             <1..938
                     /locus_tag="AC06IO_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876041.1"
                     /translation="TNCKTSDTLLLKPILGYSCFNFSISVFILILFYIPLTSGTCWAK
                     KSVNHNEVVMRGKREFLKHEYSPGHWSIDYTRAGTSIAVITVRNKYHYSVILNPTDCR
                     GYRIIIRYLNEGDSSLSSAFNRPYTVSEQRGLNDVASLMTQVYEKLGLIVQFSQLGNN
                     SQSFDKETGVTLIGSEEEPSMLHLHMWGRGDPDMEYIAGVPLRGPEPGLMFDLIAKNK
                     THPINQHAIKWNEDELKACLAMFKLKLAEYVNSPEFTEEFGDTLKVTIHDKKDLGEAK
                     ESSFGFFHKMKSSSEDLTTLDKTLETRDDKINPFG"
BASE COUNT          581 a          300 c          321 g          509 t
ORIGIN      
        1 tgacaaactg taaaaccagt gacactttgc tattaaaacc cattctggga tatagttgtt
       61 ttaatttttc tataagtgtc tttattttaa ttttgtttta tatcccgctg acatcaggca
      121 catgttgggc aaaaaaaagt gtaaatcaca atgaggttgt aatgagagga aaaagagaat
      181 ttttaaaaca tgaatactcc cctggccatt ggtcaattga ttatactcgg gcaggcacct
      241 caatcgcagt gatcactgtc cgaaataaat atcattactc tgtgattctt aaccctaccg
      301 attgtagagg ttatcgtatc attattcgat atctgaatga gggggattca agcctttcat
      361 ctgcttttaa ccgcccttat actgtatctg aacaacgcgg attgaatgac gttgcaagtt
      421 tgatgactca agtctatgaa aaattagggc ttattgtgca gttttctcaa cttggcaata
      481 actcccagtc ttttgataaa gagacaggag taacgctcat tggaagtgaa gaagaaccca
      541 gtatgcttca tttgcatatg tggggcaggg gcgatcctga catggaatat atcgccggtg
      601 ttcctcttag agggcccgaa cctggtttga tgtttgattt aattgcaaag aataaaactc
      661 accctattaa ccaacatgcc ataaaatgga atgaagatga gctcaaagcc tgtcttgcca
      721 tgttcaagct taaactggct gaatacgtta attcaccaga gtttactgaa gaatttggcg
      781 ataccctaaa agtgactatt cacgacaaaa aagatcttgg cgaagcaaag gaaagtagtt
      841 ttggtttttt tcataaaatg aaatcttcat cagaagactt gaccactttg gataaaacct
      901 tagaaacaag agatgataaa ataaatccct tcggataatc gacagagcaa tataaatgat
      961 atcgtcttta ttgtactcga atttcagccg ggaaaagtat caaaatttcc ggataaaaat
     1021 gttttcagcc agccactatt aaccatacgc atccactgtc tacctgttgt tgctactgcc
     1081 aacttgatag tggataatct tatggaacac atatctgcag atttttgatc tgttcgaatt
     1141 caatttatca acagctcaat tgatgctcat caaaaaaact gatgaatatg tttaaaacac
     1201 ttgtatacac tctaccagag tggcatttat tagcattaaa cctctggcat aacctgttta
     1261 ttttatttca ggacaagggg aaaacgtctt taaataagca ccacagaaaa gttcgcaacg
     1321 cggcagtaaa ataaaaataa caggttatgc aaagcagtct attcgtctcg gcaaagagtg
     1381 catcaacaca tacaacgaag tataagctat aggtctatta tgaaagccac ggaacctatg
     1441 gagaagagaa gttgcttgtc tttaaaaaaa agactgacga aaaactccaa ggtcaaggca
     1501 aagaattttt ttaatgaggg tgtttagata aactaaatga ccgaattaaa aacatttttt
     1561 aacatagaga ttggggtttt tcataagtcc tggaaaattt aaaaacgcgc tagacaatat
     1621 aaattaaatt tgagtgcaaa aatcaatctt gttcagagtg atttattcga aaagatatat
     1681 tcttaatgca aaatttccgt ggattggatg c
//
LOCUS       DBNCWK010000245         1673 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.246, whole genome shotgun sequence.
ACCESSION   DBNCWK010000245 DBNCWK010000000
VERSION     DBNCWK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.246"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..282
                     /locus_tag="AC06IO_002886"
     CDS             <1..282
                     /locus_tag="AC06IO_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector VdpA"
                     /protein_id="HIO9876042.1"
                     /translation="IEIGHFLIFVFKAALKNYDKHDFILLLKNLKYLHHSIELIRNKP
                     FHSDDRFYGQWSFEGHDPKRILEFIKSDDISGLMTILEDKKALPNNKPN"
     gene            complement(385..1212)
                     /gene="lem24"
                     /locus_tag="AC06IO_002887"
     CDS             complement(385..1212)
                     /gene="lem24"
                     /locus_tag="AC06IO_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem24"
                     /protein_id="HIO9876043.1"
                     /translation="MWIFKDITDRYYKKILDSYQLQADEKTYKGKEAELLIQRNELAR
                     CLIKTISLMEVKTQEDYANYFKRVSDEINSALQAAKNAVKEYNEEHNTDFTTDLYESF
                     FTYSLSNFIEVVSDLFQKSPSIITNIMDESLFCNSRSVPWVYQLSFVFYDYILDKEFE
                     FITTKSGRDIFDAKKQLVLKYIQKAADLYPRYEKEDFSDCHEMVNILLLSMRSEENNL
                     QKRRNQDSSTSYMYSYFTQTLYNASGKLMGKGQLGQKIDLLIEEFNERVVNKTPLYT"
BASE COUNT          564 a          274 c          258 g          577 t
ORIGIN      
        1 attgaaattg gacatttttt gatatttgtt tttaaagcag ctttaaaaaa ttacgataaa
       61 catgacttca tattactatt gaaaaacctg aagtatcttc atcattctat cgagttaatt
      121 agaaataaac cgtttcattc tgacgaccga ttttatggac agtggagttt tgaagggcat
      181 gatcccaaaa gaatcctcga attcattaaa agcgatgata tttcaggatt aatgactatt
      241 ttagaagata aaaaggcgct tcccaataat aaaccgaatt aacaggttta aatccaaagc
      301 gtcactgagt taacaaatca acttgaattg gtgtttttac tattaatcca gtgacctttc
      361 tgtcttgctt tggaaaataa agaattaagt atacaagggt gttttattga ctactctttc
      421 attaaattct tctatcaata aatctatttt ttgccctaat tgccccttcc ccattaattt
      481 cccggaagca ttataaagtg tttgtgtaaa ataagagtac atataacttg tactactatc
      541 ctgatttcta cgcttttgta aattattttc ttcagaacgc atgctgagta acaggatatt
      601 caccatctca tggcaatcag agaaatcttc tttttcatag cgaggataaa gatcagctgc
      661 cttttggatg tatttaagaa ccaactgttt ctttgcatca aaaatatctc tgccgctttt
      721 agtggttata aactcaaatt ccttatcaag gatatagtca tagaatacaa aagaaagctg
      781 atacacccag ggtacgcttc tactattgca aaacaatgac tcatccatga tattagtaat
      841 aatagaagga cttttttgaa acaggtcaga tacgacttca ataaaattgc ttaaactgta
      901 tgtaaaaaaa ctttcataca aatctgttgt gaaatcagta ttatgttctt cattatattc
      961 tttgacagca tttttggctg cttgtaatgc tgagttaatt tcatcagaga ctcgtttaaa
     1021 ataattagca taatcctctt gggttttaac ttccattaat gaaatggtct taatcaaaca
     1081 tctggcaagc tcatttcgtt gaattaataa ttccgcttct ttgcctttat atgttttttc
     1141 atctgcctgc aattggtagc tatccaaaat ttttttataa tagcggtcgg taatatcttt
     1201 aaaaatccac attatctacc tctttaatta gtgatccaga aagtatttgc aaaaattgtc
     1261 gctgattatc aagaacaata acgtcttcct tttctattac gggcgatagt caatattgta
     1321 atgacagcaa acacatgatt tccttgtctg atgtattggt aatccattga taactgccaa
     1381 gaattataaa taaatgttca aaaaacagta ggaatagcca tggtgtctaa tagtaactca
     1441 ttgtaagtta taggaaaagt atctgtattc ggttttttgg aaatagttga agacaaaaaa
     1501 cactagagag aaatatactt acaaggctta aaaacagtat tgatttacaa agcaattatt
     1561 tggctctttt ctaaaaaaga acgataatta atcaagagtg gtaaggttac caatttgcag
     1621 aactcaaata cggtttttgc tctggagctc ttacataacc aggatagaat cgc
//
LOCUS       DBNCWK010000246         1645 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.247, whole genome shotgun sequence.
ACCESSION   DBNCWK010000246 DBNCWK010000000
VERSION     DBNCWK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.247"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            494..1348
                     /locus_tag="AC06IO_002888"
     CDS             494..1348
                     /locus_tag="AC06IO_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg2555 family Dot/Icm T4SS effector hydrolase"
                     /protein_id="HIO9876044.1"
                     /translation="MELTMAKELIFIDWDDTLVNRENAEDFESVIDENNTIYQMSPMF
                     IGAVEALKQLQEKYHVVIVSSRGDLNRIRQLQLQSYDLESSIMPLDSFTFEDNDNRLL
                     FTRYGETNFPDKASFILYIQKMSGNKIKVFIDDDHTEIEAAQSKGIPTIHATHNKYLQ
                     NTEQFFSGLIHQVDNMELSHIFNKVAAEIRNNSRSISWGRQIAGFFSVHLPIAQSLEN
                     LASKLQRKSANEVSEEFDRYLKKIAYDPNKKGDIAVSLTKAKTAIDKHISAPIEIKEG
                     QLFLNRMG"
BASE COUNT          585 a          267 c          272 g          521 t
ORIGIN      
        1 aactgtcgaa attgaacagt tatggcactt attttttacc tataatcact ccgacaactt
       61 gtttctgttt ctattactta agctacggga tttaataaat gaaaattact ggagcaatta
      121 agatgatcca aacacatgga cgagcagaca gatttgtgaa aaagcaacta ccaaatgatt
      181 ggtttttagc actcgaaatt actgattagg aagaaatact gataatgatc ttgaacagca
      241 aaagattgat accgcattat tgattaattg taggtttcgt catagggttg aacctaaaat
      301 aatggatgaa aaaaatttaa aaaaccttca tttacccctg ggattactct gctcctattc
      361 tttttacact tagtttattc ctatttcact gctaaaaaat gacagttatt tctattgctc
      421 taccgggcta taattgtaaa aaatttaatc tttatgatct tgcgattgtt aaattaaatt
      481 atttaagatt aatctggagt taaccatggc caaagaacta atttttattg attgggatga
      541 tacattagtc aatagagaga acgcagaaga ttttgaatcg gtaatagacg aaaacaatac
      601 tatttatcaa atgtctccta tgtttattgg ggcagtagaa gcgctgaaac aacttcaaga
      661 aaaatatcat gttgtgatcg tcagttcacg aggagactta aataggataa gacagctcca
      721 attacaaagt tatgatctag agtcttcaat tatgccatta gacagtttca catttgaaga
      781 caatgataat cgtttattat ttacaaggta tggtgaaaca aactttcctg ataaagcaag
      841 tttcattttg tatatccaaa aaatgagcgg taataagata aaagtattta tcgatgatga
      901 ccatacagaa atagaagccg cacagtcaaa aggaataccc acaatacatg cgactcataa
      961 taaatatctg caaaatacag agcaattttt ttcaggatta attcatcaag tagataacat
     1021 ggaactttcg catattttta ataaagtagc agctgaaata cgtaacaatt ctcgttcaat
     1081 ttcctgggga aggcaaattg ctggtttctt ttcagttcac ctgcctatag cacagagttt
     1141 agaaaatttg gcatctaaat tacaaagaaa atctgctaac gaggttagcg aagaatttga
     1201 tcgatacctt aagaaaattg cttacgatcc aaataaaaaa ggggatatag ccgtaagttt
     1261 aactaaagct aaaacagcaa tagataaaca tatatcagcc ccgattgaaa tcaaagaagg
     1321 tcaactattt ctcaatagaa tgggttaaca atcctttcca ttatccaggt gccttggtat
     1381 gctccaggaa ctactgtcat tcactttagc ttcctcaatc gacatcccgg ggcttgtcct
     1441 cgggatttaa aatttgtgag atcctgcaaa caaatctcga gatttagata tatgtatcga
     1501 tatctaaagt caatagcaat gctccaagga gaaatacatt gatttctgta ataatcaata
     1561 tttcaatcat ccagttgcga tttaatttta tgctgttgac ttattgaata atgattctct
     1621 acatcatttt tatgaagttt aaaat
//
LOCUS       DBNCWK010000247         1618 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.248, whole genome shotgun sequence.
ACCESSION   DBNCWK010000247 DBNCWK010000000
VERSION     DBNCWK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.248"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(261..1118)
                     /gene="queF"
                     /locus_tag="AC06IO_002889"
     CDS             complement(261..1118)
                     /gene="queF"
                     /locus_tag="AC06IO_002889"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945780.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HIO9876045.1"
                     /translation="MNKELESTYQNITNQSELGQSSNYDDHYNPKRLYPIPRAPKRQE
                     INLDTNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNS
                     LNNTTFPDVETVVQTISKDLSHCIGSEVAVNVYPLSEIASQTIYAAFDGICLDKLDIE
                     CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLK
                     YLISFRNHNEFHEQCIERIFADIMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCVFD
                     GQNIRLIRQ"
     gene            complement(1359..1511)
                     /locus_tag="AC06IO_002890"
                     /pseudo
     CDS             complement(1359..1511)
                     /locus_tag="AC06IO_002890"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT          520 a          292 c          287 g          519 t
ORIGIN      
        1 tttttgcctt gaccttgcgt caatcaaaat aaacacccaa accaagtttt tattctttaa
       61 tctatggatt tcagcgatgg accaatcgaa cagcgatgag taaatctgtg gaattatgtt
      121 taagatttat gcctgaattg gtacaaattt tcaggcaatc ttagcatagg aaagatgtaa
      181 tgtattgaaa attaattatt aaattcccag gcgggattaa aacttattcc ttatcctgct
      241 ggaggggaaa gtaccctcct ttattggcgt attaaacgaa tattttgccc atcaaaaaca
      301 caaggttctg ttgacctgat tggattaata tccagacctc cccttcttgt atatcgccca
      361 taaacagtca atgattcagg ttggcaaaat cgcataatgt ctgcaaatat ccgttcaatg
      421 cactgctcat gaaattcatt atgatttcta aatgaaatca ggtatttaag caacccttca
      481 tgatttatct ttttaccttt ataaataatt tgaacgcttc cccaatccgg ttggttggta
      541 accaggcagt ttgatttaag taaatcagaa tacaatacct cttcaactaa ttcagacgag
      601 gtcgaaagaa aatcaggcat tacatgatat acagaacatt caatatcaag cttatcaagg
      661 caaataccat cgaatgcagc atagatagtt tgactagcga tttccgacaa ggggtacacg
      721 ttaaccgcca cttcagaacc aatgcagtga cttaaatctt tgctgatagt ttgcacaacg
      781 gtttccacgt cagggaaagt agtgttattc aacgagttaa aatagagttt taatgacttg
      841 gattcaataa tacacgggga atgacaatca taagaaataa cagccatcgc aacaacaggt
      901 ttacctttgg agtttaacca ggacacctca taatgattcc agcaatcaaa accatagaaa
      961 gtggtggagt tggtatccaa attaatttcc tggcgttttg gggctctggg gataggatac
     1021 agccttttag gattatagtg gtcatcataa tttgaacttt gtcccagctc cgattgattc
     1081 gttatattct gataagtgct ttcaagttcc ttattcatta ttgatgcctc caaaatagta
     1141 atgatttgcg ctgtactata ccaattgatt ggctgcccgg cttcaatttg cattgcaatg
     1201 tagtttaata aagaagacgc ttcatgaatt tgctctgctt ttttctctaa agccttggct
     1261 tgtacagcaa gtgaagtttt tagattaaaa tcaggatgct gaataatttc tttgattgaa
     1321 gcaagtgaac atcccaggta cctcagggta gttatttgct gcaagcgtaa taaatcatcc
     1381 gatgaataca gtcggtaatc cgactgtgac cgtttcgatg gctttagtaa attaatttca
     1441 tcataatatt gtaaggtacg aatacttaat gaagtcagtt tgctgatttc tttaatctta
     1501 taaaatttca ttttgtaact ctaaaaaaca aaatgattat aatctatcac gttacgtgag
     1561 ggtcaatatt ttattaaaaa aatgaaataa actatattta caggacttac gaaacacc
//
LOCUS       DBNCWK010000248         1542 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.249, whole genome shotgun sequence.
ACCESSION   DBNCWK010000248 DBNCWK010000000
VERSION     DBNCWK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.249"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..573)
                     /locus_tag="AC06IO_002891"
     CDS             complement(<1..573)
                     /locus_tag="AC06IO_002891"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIO9876046.1"
                     /translation="MPEFTLIQQICIWALPVLLAITFHELAHGYVAYRCGDNTAKMFG
                     RLTLNPIKHIDPIGTVLLPLLIGVITNFNFVIGYAKPVPVNQSQLRKPRRDMILVTLA
                     GPLANLIMAFLWAGCFKIATFLNPQSSMAALFLLVTARAGMMINLILAFLNLIPIPPL
                     DGSKVVINLLPARQAIAYAKLEPFGFLILIV"
     gene            830..1468
                     /locus_tag="AC06IO_002892"
     CDS             830..1468
                     /locus_tag="AC06IO_002892"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIO9876047.1"
                     /translation="MTQFEKTFNFMKKIWVIITYIILVVLSYYFLDRTLATYFHSLDL
                     RVNAHALTYLTALGKWKIYVALFLITALYFRYIQQNKQYEIRSWYLLGCVLLPNLLTF
                     VLKISFSRARPDLLFDNNLYGFYWFQHNDNYWSFPSGHSITITALAAGLGFLFPRYFF
                     LFIGAALLVAATRVILYHHYLSDVMTGFYISMLLVGLFTQYLQRNHYLNKIN"
BASE COUNT          460 a          253 c          295 g          534 t
ORIGIN      
        1 tacaatcaat attaaaaaac caaatggttc taattttgca taagcgatag cttgtcttgc
       61 tggcaataga tttattacaa ctttgctgcc atcaagtggc ggaatgggga ttaagtttaa
      121 aaaggcaaga atcaaattga tcatcattcc cgctctggct gtaacaagca ggaataaagc
      181 agccattgag ctttgaggat ttaagaaagt tgcaatttta aaacatccag cccataggaa
      241 tgccattatc agatttgcta atggtcccgc aagcgttacc aatatcatat ctcggcgtgg
      301 ttttcgcagt tggctctggt taaccggaac cggttttgcg taaccaatta cgaaattgaa
      361 atttgttatc acgccaatta aaagaggtaa tagaacagtg cctattggat caatgtgttt
      421 gatagggttt aatgttaatc gaccgaacat tttagctgtg ttgtcaccgc agcggtaggc
      481 aacgtaacca tgagctaact catgaaaagt aatagctaat aatactggta aagcccaaat
      541 acaaatttgt tggattaacg tgaattctgg cattaatatt ctgatgtatt taataaggat
      601 cctgatttta acgaattcta ctcgctaata acaagggttg atgaaaaatt gtgtggccgc
      661 tcgcatttgt tgatattttt accatccttg ccgaaaaatc ttgattttct actcaagcgt
      721 gagaaatctc gacaggacct aatttttatt aataacatgg ggtcttggct aaatcgaaag
      781 catcttatat gattccattt gatatgagga tttataaggc ttggtttcga tgacgcaatt
      841 tgagaaaact tttaacttca tgaaaaaaat ctgggtaata ataacctata ttatccttgt
      901 tgttctctct tattattttt tggatagaac gttggctact tatttccata gcctggatct
      961 tcgagtcaat gctcatgcat taacatactt gacggctttg ggaaaatgga agatttatgt
     1021 tgcattgttt ttaatcaccg cattatactt tcgatacatt cagcaaaata aacaatatga
     1081 aattagatct tggtatttat tgggctgtgt tttgctgcct aatttattga cttttgtgtt
     1141 aaaaattagc tttagtcgtg cgcggccgga tctcttgttt gataataacc tctatgggtt
     1201 ttattggttt caacacaatg acaattattg gtcattccct tcagggcatt ctattactat
     1261 aactgctttg gctgcgggat tggggtttct cttcccaaga tattttttcc tctttatcgg
     1321 agcagccttg cttgtcgctg ctaccagggt tattttatat catcattatt taagtgatgt
     1381 tatgactgga ttttatatca gtatgttgct tgttggctta tttacacagt atttgcaaag
     1441 gaaccactat ctcaataaga taaattagac tctataaatc gtagtcaaat ttattaaggg
     1501 agtaattatg actatagaaa gggaattaaa aaattttgta ag
//
LOCUS       DBNCWK010000249         1538 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.250, whole genome shotgun sequence.
ACCESSION   DBNCWK010000249 DBNCWK010000000
VERSION     DBNCWK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.250"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            232..408
                     /locus_tag="AC06IO_002893"
     CDS             232..408
                     /locus_tag="AC06IO_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIO9876048.1"
                     /translation="MQEYKRYICVICGFIYDEAEGWPEDGIEPGTRWEDVPENWFCPD
                     CGAGKEDFEMVEMD"
     gene            complement(405..827)
                     /gene="hemJ"
                     /locus_tag="AC06IO_002894"
     CDS             complement(405..827)
                     /gene="hemJ"
                     /locus_tag="AC06IO_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIO9876049.1"
                     /translation="MLFVKAFHIIALVAWFAGLFYLPRLYVYHAESLDKISVDRFKIM
                     ERRLYYGITWPAALATTFLGLWLFTYNLSYYLKAGWMHMKLALVLLLWVYHLTCGHFL
                     KSFALGKNTRSSRFYRFFNEMPTLLLVGIVLLVVVKPF"
     gene            complement(830..>1538)
                     /locus_tag="AC06IO_002895"
     CDS             complement(830..>1538)
                     /locus_tag="AC06IO_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIO9876050.1"
                     /translation="SLTALIILEVVLGIDNLVILSILTEKLPREKRKKARRWGLTFAW
                     MTRLLLLGSAVFLVKLVKPILTIGSLVFSARDLFLILGGAFLIWKSTDEIHQDVMNEP
                     LMEPVSKNVSSSATFRGVVIQIALLDIIFSLDSVLTAVGLTSHFMVMAVAITIAIIIM
                     IWASEPVSKFISEHPTIKMLALSYLILIGTVLVADGFEFHVPRGYLYFAMGFSLAVES
                     LNLIKNERVKRKKLKRE"
BASE COUNT          538 a          280 c          268 g          452 t
ORIGIN      
        1 attacaatca tttcatgaat attgcaaaaa atagtggcag cacctgtctt taaaagataa
       61 tcacattggc tggtttttga ggttgatttt agcaaaccca tgccaattct cgacaaagtt
      121 ataatgttca ttataaggtt taatttaaaa tttattatga tattaaaaag gtttaacttt
      181 acataatcat caacagtaga taaattagca cttttttatt gagtttagac tatgcaagag
      241 tacaaaagat acatttgtgt tatttgtggt tttatttatg atgaggctga aggttggcca
      301 gaagatggta ttgagccagg cacacgatgg gaagatgttc ctgaaaactg gttttgtcct
      361 gattgtggcg caggaaaaga agatttcgaa atggttgaaa tggattaaaa tggttttaca
      421 accaccagca aaacaatgcc taccagcagc aatgtaggca tttcattaaa aaatcgataa
      481 aaacgagagc ttcttgtatt ctttccaagg gcgaatgatt taaggaagtg gccgcaagtt
      541 aaatgatata cccataagag caaaaccaat gcaagcttca tgtgcatcca ccctgcttta
      601 agataataag ataaattgta agtgaatagc cacaacccaa gaaatgtggt tgcaagagct
      661 gcaggccatg taattccgta atataatctt ctttccatta ttttaaaccg atcaacgcta
      721 attttatcta gcgattcagc gtggtatacg tagagccgtg gtaaataaaa caaccccgcg
      781 aaccaggcta ccaaggcgat aatatgaaaa gctttaacaa ataacatact tactctcttt
      841 ttaatttttt acgctttaca cgttcatttt taatcaagtt gagtgactca actgctaatg
      901 aaaagcccat agcaaaatac aagtatcccc tgggcacatg gaattcaaaa ccatcagcta
      961 ccaaaacggt tcctattaaa atcaaataac taagcgccag cattttaatg gtaggatgtt
     1021 cactaataaa tttacttaca ggctcactcg cccaaatcat aatgataatt gcgatggtaa
     1081 ttgccactgc catgaccata aaatgagatg ttagtcctac ggccgtgaga acgctatcca
     1141 gtgaaaatat aatatcaagc aatgcgattt gaataacaac tcctctgaaa gtggcagatg
     1201 aagaaacatt ttttgaaaca ggttccatta atggttcatt cataacgtcc tgatgaattt
     1261 catcagttga cttccaaatt aaaaaagcac ctcctaatat gaggaacaga tcacgagcag
     1321 aaaataccag actgcctatt gtcagaattg gttttactaa cttaactaaa aaaactgctg
     1381 agcctagtaa aagaagtcgt gtcatccagg caaatgttaa gccccatctt ctcgcctttt
     1441 ttcttttttc acgtggtaat ttttctgtaa gaatcgatag aataaccaga ttatctattc
     1501 caagaacaac ttcaagaata atcaaagcag ttaaacta
//
LOCUS       DBNCWK010000250         1513 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.251, whole genome shotgun sequence.
ACCESSION   DBNCWK010000250 DBNCWK010000000
VERSION     DBNCWK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.251"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1492
                     /locus_tag="AC06IO_002896"
     CDS             <1..1492
                     /locus_tag="AC06IO_002896"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HIO9876051.1"
                     /translation="SIYKKSLWGLRVVDLDTGNVLLNLNSSTQFYIGSVRKIFSVGEL
                     LNERGADYQSVTTVHYDGVLHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNE
                     ANSLGNAIITQTNLLAGYQNLAKQVYKSGIRSISGDIVIDDRLFEPFNFRGEFDVTPI
                     FINDDVVDIVILPSLINENANVSWLPHSSAFTVINQLLTVNPHEKYNLELESKLPTCI
                     GKPGCSGIIKGHLPINFIPPLTHSYPLVQTFRITKPSNYARTIFIEALQEAGINVSSI
                     DKTKENPKKLLKSTNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTK
                     GARSMIESLNVEKKILDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVF
                     NIFYNSLPILAVDGSLGSINNFQSDPSLSGATGRVHAKTGTYVLGNESGMLVKGRSLA
                     GYIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY"
BASE COUNT          494 a          271 c          283 g          465 t
ORIGIN      
        1 atctatttat aaaaagtcgt tgtgggggtt acgtgttgtt gatcttgata ctggtaatgt
       61 attgcttaat ctcaactcaa gtactcagtt ttacattggt tctgtgcgca agatatttag
      121 tgtaggcgaa ctattgaatg aaagaggagc tgattatcaa tctgttacta cagtccatta
      181 tgatggtgta ttacacaatg gtaatctgaa tgggaatctg gtactcgttg cctctggtga
      241 tttgaccatg ggaggacgaa ctttacctga tggacatgtc gctatcactg aatacgatca
      301 taacgaggca aattcattgg gtaatgcaat tattacacag actaatcttt tagcaggtta
      361 tcaaaatctc gctaaacaag tttacaaaag cggcattcgc tctatttcag gcgacattgt
      421 gatagacgat agattgtttg aaccatttaa tttcaggggt gaatttgatg taacacctat
      481 tttcataaac gatgatgtag ttgatattgt aattcttcca agtttgataa acgagaatgc
      541 aaatgtatcg tggctgccac attcatcggc gttcaccgtt atcaaccaac ttcttacagt
      601 taatccgcat gaaaaatata atcttgaact tgagtcaaaa ttacctacct gcataggtaa
      661 accaggctgt tctgggatca ttaaaggcca tttgcctatc aattttatac cgccacttac
      721 tcacagctac ccattagtac aaacttttcg aatcactaaa ccatctaatt atgccagaac
      781 aatttttatc gaggcactac aagaagcagg catcaatgtt tcatcgattg ataaaaccaa
      841 agaaaatccg aaaaaactac taaaatctac taacttgtac aataagaata acaaaattgc
      901 tgctttgaaa tcattgccat tgggagagca tgtaaaatat attctcaaag ttagttataa
      961 tatcggagca gataccagcc tggtgttata cggactaaca aaaggcgcgc gctccatgat
     1021 tgaaagtcta aacgtggaaa aaaaaatcct tgataaaaaa tatggcatat ccccatcaga
     1081 atatcatttt attgatggca gcggcggcgg cgaaacgaaa gcaaccaaca ccgctataat
     1141 aaaatggctt actataatgt ctaaaagacc agtatttaac atattttaca atagtctgcc
     1201 tattcttgca gtagatggtt cacttggttc aattaataac tttcaatcag atccatcgct
     1261 ttctggggcg acaggtcgag tgcatgcaaa aactggaact tatgtattgg gaaatgagtc
     1321 gggtatgtta gttaaggggc gctctcttgc tggttacatt attagtaaaa aaaatcaccg
     1381 acttgcattt gccttagttg ttaataatat acctatacag tcactagaag atttatttgc
     1441 tgtagctcag gatcaaggca taatagcatc aattctatgg cgagattatt gattattaat
     1501 ataataatat ttc
//
LOCUS       DBNCWK010000251         1503 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.252, whole genome shotgun sequence.
ACCESSION   DBNCWK010000251 DBNCWK010000000
VERSION     DBNCWK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.252"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            15..>1503
                     /gene="lbtA"
                     /locus_tag="AC06IO_002897"
     CDS             15..>1503
                     /gene="lbtA"
                     /locus_tag="AC06IO_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore legiobactin biosynthesis protein
                     LbtA"
                     /protein_id="HIO9876052.1"
                     /translation="MAAVLNNSYKINNSLHSLLLEKDLLVSQSEMEHFISIAHQQCLD
                     RLQQAALREGLIDKEITSLHIHDYLDLLYHQQKKQKPEKTFDRWPLLKKELDESINNQ
                     ALALAYYHCWQQSLKQQAKNHSSLWSWLSEQHNKQQILTFLEQWGCIGHPYHPNFRAK
                     MGFIPEEVIRYSPEFNAQVDIPWLAIHRSLAFTSIDATSYSLLFAQHFAKEYQLWNYS
                     VQKQQLDPEQYLPLPVHPWQWNNKLQTLASVLINSKQLLITEAFQKTKPSMSFRTMMP
                     LDNKGPHLKLATAIHTTSALRTVSPASVSNSSPLSSWLRQLLTQYQSFEKPLFLARDL
                     AGINTTHTSISPQNKKHLAMIVRENPLELIDASQTLVPLAALYALSPVSQRALFIEII
                     EASGMEPELYFIEYSRCILECQLHLLLSYGVALEAQQQNTLVIFQSHRPVGLVIRDLG
                     GIRVCFNSLYETTPKPNLHPDSTISCHELSDLFNTFIYGNLLSNLT"
BASE COUNT          479 a          327 c          246 g          451 t
ORIGIN      
        1 tcacggaaat aatcatggca gcagttttaa ataattctta taaaataaat aactcattac
       61 acagtttatt acttgaaaaa gaccttcttg tttcacaatc agaaatggag catttcatat
      121 ccattgcgca ccagcaatgt ttagatcgat tgcaacaggc agcacttcgt gaaggattaa
      181 tagacaagga aataaccagc cttcatatac atgattatct tgatttactt tatcatcaac
      241 agaaaaagca aaaaccggaa aaaacatttg atcgatggcc tcttctaaaa aaggaactcg
      301 atgaaagcat caacaatcag gcgttagccc tcgcttatta tcattgctgg caacaatcgc
      361 tgaagcaaca ggcgaaaaac cactccagtt tatggtcatg gttatcagag caacataata
      421 aacaacaaat tctgactttt ttagagcaat ggggttgcat agggcacccg tatcatccta
      481 atttccgcgc aaaaatgggg tttattcctg aagaagtgat tcgatactcc ccagaattta
      541 acgcgcaggt agatattccc tggctggcaa ttcatcgctc cttagcgttt acttcaattg
      601 atgcgactag ctattctttg ttgtttgccc aacattttgc taaagaatat caattgtgga
      661 attattctgt ccaaaaacaa caattagatc ccgaacagta tttgcctctt ccagttcatc
      721 cctggcaatg gaataacaaa ttacaaacac tggcctcagt attaatcaat tcaaagcaat
      781 tattaataac agaggctttt caaaaaacca aaccatccat gtcgttccga acgatgatgc
      841 ctttagataa caaaggtcca cacttaaaat tagctacagc aatccatact acttctgctt
      901 tacgcactgt atctcctgca tcagtaagca attcatctcc tctttccagt tggcttaggc
      961 aattactgac tcagtatcaa agttttgaaa agccactatt tctagccagg gatttagcag
     1021 ggattaacac cacacacaca tccatttctc cacaaaataa aaagcatttg gctatgatag
     1081 tacgtgaaaa tccactggaa ttgatcgatg cgtcacaaac attagtacct ttagctgctc
     1141 tctatgctct ttctccagtc agtcaacgtg ctttattcat tgaaattatt gaagcaagtg
     1201 gcatggagcc tgaattatat tttattgaat acagccgatg tattctcgaa tgccagcttc
     1261 atttattatt aagctatggc gttgcgttgg aagcccaaca acaaaacact ttagtcattt
     1321 ttcagtctca tcggcctgtt ggattagtta ttcgagatct tggcggcatc agggtctgtt
     1381 ttaattcgct atatgagaca acacccaaac ctaatcttca tccagactct accatttcat
     1441 gccacgaatt gagtgatctc tttaatacct tcatttatgg aaatttactg agtaacttaa
     1501 ctt
//
LOCUS       DBNCWK010000252         1458 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.253, whole genome shotgun sequence.
ACCESSION   DBNCWK010000252 DBNCWK010000000
VERSION     DBNCWK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.253"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            10..>1458
                     /locus_tag="AC06IO_002898"
     CDS             10..>1458
                     /locus_tag="AC06IO_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876053.1"
                     /translation="MTTPLFPKNDSFVTIRQGKTGDCYLLAALDCILNSGPEGYATIK
                     SLFKETKDGIEVRFKSSDLSQDLQKDKLQGKYKYHYDPINNQDVFFIDKKKIQEIDQT
                     TDGVKSNSLAIKILERLSSYYLASDWNANLPLASIVAHNGSKGSKRHNETSTKFVGQL
                     LGFMAHDTRDFHEIIRLKTTAPQEPVYISIKYGEVDSNGVRHGRHALRLDRVIPNPKS
                     AGGYDFVLVNPWNNQLKETYSLDEIQQRKCRFSIFSTKPYKNDLKPYEHDLKEKLFKV
                     YQKLGKKIFDNDKLYQMILKMSEVNGKLLDLRTISKCVKLYERIGGFSNLFDALSLSQ
                     QKAMISCVDLNTKPTQFIKSFLSSAPSARSAVFENLDLTKYYVNKISQYPIDSVNFDD
                     SVESVNKYKESIISELQKIKDRAFAQKKYIQLNNATLEKALAEKLREVHALFRFGLFF
                     KPQNIDSNQELNINPNQELNINPNQELNIDPNQ"
BASE COUNT          548 a          234 c          246 g          430 t
ORIGIN      
        1 gattaaatca tgacaacacc actattcccc aaaaatgaca gttttgtaac tatacgtcaa
       61 ggtaaaacag gagactgtta tcttttggcg gcgttggatt gtatactaaa ttcggggcca
      121 gaaggttatg caaccatcaa atcattattt aaagaaacta aagatggcat tgaagtaaga
      181 tttaaatcta gtgatctgag tcaagattta caaaaagata agttgcaggg taaatacaaa
      241 tatcattacg atccgatcaa taatcaagac gtgtttttta ttgacaaaaa gaaaatacaa
      301 gaaattgacc aaactactga cggagtaaaa agtaactctt tggccataaa aattttagaa
      361 cgcttaagtt cttattactt ggcatcggat tggaatgcaa atctaccatt ggcaagtatt
      421 gttgctcata atggatcgaa gggatcaaaa aggcataatg agacctcaac gaaatttgtc
      481 ggtcaattat tgggttttat ggctcatgat acccgtgatt ttcatgagat catacgattg
      541 aaaactacag ctcctcaaga gccagtttat atcagtataa agtatggaga agttgattct
      601 aatggggtaa gacatggccg acatgctttg agacttgata gagttattcc taatccaaaa
      661 agtgcgggtg ggtatgattt tgtcttagtc aatccatgga ataaccaact gaaagaaact
      721 tatagtttag atgaaatcca acaaagaaaa tgtcgtttct ctatatttag caccaagcct
      781 tataagaatg atcttaaacc ttatgaacac gatcttaaag aaaagctatt caaagtctat
      841 caaaaattag ggaaaaaaat atttgacaat gataaacttt atcagatgat tctaaaaatg
      901 agcgaagtga atggcaaatt attagattta agaacaatta gcaaatgcgt taaactatat
      961 gaaaggattg gtggtttttc caatctgttt gacgcgttat ctcttagcca acaaaaggca
     1021 atgatttcat gtgttgacct aaacacaaaa ccaactcaat ttataaaatc attcctatct
     1081 tcagcaccct ctgctcgctc tgcagttttt gaaaatctag atttaactaa atattacgtg
     1141 aataagatta gtcaataccc tatagactct gtgaattttg atgactctgt agaatcagtt
     1201 aataaataca aagagtctat aatatctgaa ctgcaaaaaa taaaggaccg tgcatttgct
     1261 caaaaaaaat atattcaact aaataatgct actctcgaaa aagctcttgc tgagaaatta
     1321 cgtgaggtac atgcactgtt ccggtttggg ttatttttta aaccacagaa cattgattca
     1381 aatcaagaat taaatatcaa tccaaatcaa gaattaaata tcaatccaaa tcaagaatta
     1441 aatatcgatc caaatcaa
//
LOCUS       DBNCWK010000253         1455 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.254, whole genome shotgun sequence.
ACCESSION   DBNCWK010000253 DBNCWK010000000
VERSION     DBNCWK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.254"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            114..701
                     /locus_tag="AC06IO_002899"
     CDS             114..701
                     /locus_tag="AC06IO_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIO9876054.1"
                     /translation="MKTHTKLANIESLLEKPVFTSKEANELGVSPSLLCYYMQKDLIQ
                     RVNKGLYQAVSVKKEFDFKWEDLIFTVKSISQGVICLVSALAIYELTDEMPRKHWIAV
                     PHGTTKPMRPNTIIKRMRDVSTGLTTWDIDGESVPIFDMERTIIDAFRFLSKEIALKA
                     LQNGLREKHVDSRKLQKYAKKLRVNIAPYLLAMTI"
     gene            698..>1455
                     /locus_tag="AC06IO_002900"
     CDS             698..>1455
                     /locus_tag="AC06IO_002900"
                     /inference="COORDINATES: protein motif:HMM:NF020419.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIO9876055.1"
                     /translation="MNEQSLKAKLKQLASIKEKTFQELWKILALERFLARLAKSNLDD
                     QLVFKGGFLLAQLVEIGRETTDLDFLARNIQAAQESIVPLVETICAVELADGFIFEVM
                     KVSDLEHAHMHYPGFRLSLKVQFYSMKDTIQLDIGVGDLVEPIERSLLLTEVKNQALF
                     ESEITLLTYPIEFIFAEKLETLVSRGATNSRMKDYHDLLIILRNYELNDFQNLQEVIT
                     NTFKHRGTELVLPILFDQSEVEVLQSFWKRHINVL"
BASE COUNT          488 a          223 c          284 g          460 t
ORIGIN      
        1 cataataatt ttattttaat aaatacaccc aaaaataatt attgggttta tttgttaaaa
       61 tttaaaaaat gaacccattg gatttaattg ggtttatttg ttaaaatata aacatgaaaa
      121 cacatactaa gcttgctaac attgaatctc ttctggaaaa gcctgttttt acgagtaaag
      181 aggctaatga acttggcgtg tctccaagtt tgctttgtta ctacatgcaa aaagatctta
      241 ttcaacgagt caataaaggc ctatatcaag ctgtttccgt gaagaaagaa tttgatttta
      301 agtgggagga tttgatattt acagtgaaat caatttccca aggtgttatt tgcctcgtta
      361 gtgcactagc aatctatgag cttacagatg aaatgcctcg taagcattgg atagcagtgc
      421 ctcatggtac aacgaaacca atgagaccaa ataccattat aaaaagaatg agggatgtaa
      481 gcactggtct aacaacttgg gatattgatg gagaatcagt acccattttt gacatggagc
      541 gtactatcat cgatgccttt cgatttcttt caaaagaaat tgctcttaag gcactgcaga
      601 atggattgag agaaaaacat gttgattccc gaaaattgca aaagtatgca aagaagctgc
      661 gcgttaatat tgctccttat ttgttggcaa tgacgatatg aatgaacaat cacttaaagc
      721 aaaactgaaa caattagcat caataaagga aaagacattt caggagcttt ggaaaatact
      781 ggcattagag cgttttctcg cacgattagc caagtccaat ttggatgatc aattggtatt
      841 taaaggagga tttcttttag cacagttagt tgaaattggt agagaaacaa ctgacttgga
      901 ttttcttgca agaaatattc aagcggcaca agaaagcatc gttccgttgg ttgagactat
      961 ttgtgctgtt gaacttgcag atggttttat ctttgaagtt atgaaagtga gcgatttaga
     1021 acatgctcat atgcattacc caggatttag gctctctcta aaagtacaat tttattcaat
     1081 gaaagataca attcagttgg atattggggt aggtgattta gtggagccga ttgagcgctc
     1141 tttactctta acagaggtaa aaaatcaggc tttatttgaa tcagaaatta ctttattaac
     1201 ctatccgatt gagtttattt ttgctgaaaa gctagaaacc cttgtatcaa ggggcgctac
     1261 gaacagccga atgaaagatt accatgattt gttaatcatt ctcagaaatt atgaattgaa
     1321 tgatttccaa aatttgcaag aagtgattac aaatacattt aaacatcgtg gaacagagtt
     1381 agtcctgcct attttatttg atcagtctga agtcgaggta cttcagtctt tttggaaaag
     1441 acatattaat gtcct
//
LOCUS       DBNCWK010000254         1416 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.255, whole genome shotgun sequence.
ACCESSION   DBNCWK010000254 DBNCWK010000000
VERSION     DBNCWK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.255"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..737
                     /locus_tag="AC06IO_002901"
     CDS             <1..737
                     /locus_tag="AC06IO_002901"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase family alpha/beta hydrolase"
                     /protein_id="HIO9876056.1"
                     /translation="SIKIILMIFIGITTFVKAYAYSPDTSNPIQINRSLAEVNSRHPG
                     EVIVLIHGLMRTYVSMKPLKAYLEAQGYQVYLYKYPSARYNIQEHGVYLNQFITTILE
                     NNPAIKIHFVTHSLGGIIVREALSKLSQKQLKHVGYLIMLAPPNQGSKLAKLSIKLFP
                     MISYFIKPLAELSSEQTSYVHRVPVPDIKMGIIAGRFDAKVPPDYARLQGQTEQVIIN
                     NTHTFIMYDSKVKKLVLSFLEKGKFE"
     gene            849..>1416
                     /locus_tag="AC06IO_002902"
     CDS             849..>1416
                     /locus_tag="AC06IO_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HIO9876057.1"
                     /translation="MIIYVTMWFIVDMKELIAFIHDLYKDRTEKSVKFESKGRVIVSP
                     LMSKDDAQELFKSLKEILPEEARLKVRKSKQEPDQFRVVLFNPEKVFSFYAEHAAELL
                     NRFKPLPTSSSGEEYCKWKYNPLSHLIEYTVPFSILDSDTKAKVTDTHEEAIQSKVNQ
                     YNEKLRSIYPDLKIIGTNSVEDSSPDKFF"
BASE COUNT          478 a          232 c          247 g          459 t
ORIGIN      
        1 gaagcataaa aataattctg atgatattca ttggaataac cacatttgtc aaggcttatg
       61 cttactcccc tgataccagt aatccaattc agataaaccg ttcattagca gaagtaaatt
      121 ccaggcatcc tggagaggtt attgtattaa ttcatggttt aatgcgtacc tatgtgagta
      181 tgaagccatt aaaagcctat ctggaggctc agggatatca ggtctatttg tacaaatacc
      241 cctccgccag atacaatatt caagagcatg gagtttattt aaatcaattt attacaacaa
      301 tacttgagaa taatccggca ataaaaattc atttcgttac ccatagtctt ggtggcatta
      361 ttgtaaggga agcattatcc aaattatctc aaaaacaatt aaaacacgta ggttatctca
      421 ttatgctggc tcctcctaat caaggttcga agctggcaaa attatctatt aaactatttc
      481 cgatgatttc ctactttata aaacctttgg ctgaattaag ttctgagcaa acatcttatg
      541 tgcatcgtgt tcctgtacct gatatcaaaa tggggataat cgccgggcgg tttgacgcta
      601 aagttcctcc agactatgct cgtctgcaag gacaaacaga gcaggttatt attaataata
      661 cgcatacttt tattatgtat gattcaaaag taaaaaaact tgtgctgagc ttcttagaaa
      721 aagggaagtt tgaatagttt tttagttata gtcagcggct agtattggct taattaagta
      781 ttctatccag cttttattgt caaaaatcca ggtgtaaaaa ttgacatgtt aattaattat
      841 atctaaattt gattatatat gtgacaatgt ggtttatagt tgatatgaaa gaattaatag
      901 cttttattca tgacttgtat aaagatcgta ctgagaaatc tgtaaaattt gaatccaagg
      961 ggagagttat tgtatctcct ttgatgagta aagatgatgc ccaggaatta tttaaatcat
     1021 taaaagaaat actccctgaa gaggcacgat taaaggtacg taagtcaaag caagaaccag
     1081 atcagtttcg tgtcgttctc tttaatcctg aaaaggtttt ttcgttttat gccgagcatg
     1141 ctgcagaact gttaaatcga tttaaaccat taccgacatc ttcttcagga gaggaatatt
     1201 gtaaatggaa atataatcct ttatcacatt taatagaata tactgttccg ttttcaattc
     1261 tagattctga tacaaaagcg aaagttacag atactcatga agaggctata caatcaaaag
     1321 taaatcaata taacgagaag ttacggtcga tataccctga tctcaaaatt atcggaacta
     1381 attcagttga agactctagt cctgataaat ttttct
//
LOCUS       DBNCWK010000255         1368 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.256, whole genome shotgun sequence.
ACCESSION   DBNCWK010000255 DBNCWK010000000
VERSION     DBNCWK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.256"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            128..1348
                     /locus_tag="AC06IO_002903"
     CDS             128..1348
                     /locus_tag="AC06IO_002903"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIO9876058.1"
                     /translation="MKFTDTYIRNLKPQQKWFEQIEFSGLGIRVMPGGGKSWIFRFTF
                     DGKRYKMTLGKYPGIGLKEARELMLDAEHLKEQGINPIEYAKQQQAKSDNTVKKLAFS
                     WYTQYVEKHLKRPLTVKKQIDGDIINLLGDRVLDELETKDITEALDKIVKRGASVHAN
                     RVLSSLKQMFGYAVSRGTMSTNPASNIRARDIGGHEKPRERVLSLDEIKSLWLFLDSK
                     DSQMAPQTRIAIKIILLTGVRTAELRLAQWSEINFDDSLWTIPAMHSKASIVHKVHLS
                     DLTKQLLQQLKSISRSDYVLTGADYRQPLTENALPRAIKRIQERLGIPEWTAHDLRRT
                     FATQLGETLQVDPVVIEKCLGHKMPRIMATYNRNEMLPQRREALECWAVCIENMMQDN
                     VVYINKLLQNERSL"
BASE COUNT          442 a          258 c          289 g          379 t
ORIGIN      
        1 tatcataatt tcatgtaaat actgagtttt tattgaaatt tgactttatt ttaatatcat
       61 ttaatattat ctgaattcac atttagtggt accaaaatcg gtaccaaaaa tcatcttggt
      121 accagttatg aaatttaccg atacttacat ccgcaattta aaaccccagc aaaaatggtt
      181 tgaacaaatc gagttcagtg gccttggaat tcgggtcatg ccagggggag gtaaatcatg
      241 gatctttcgg ttcacttttg acggcaagcg ttacaagatg actttgggta agtacccggg
      301 gattggcttg aaggaagcac gtgagctgat gctggatgcg gaacatttaa aagaacaagg
      361 gattaatccc atagagtatg ctaaacagca acaagccaaa tcagataata cggtaaaaaa
      421 gctggcattt agctggtata cccaatatgt tgaaaagcac ctaaagcgtc ccttaacggt
      481 taaaaagcaa attgatggtg acatcattaa tctgcttggt gatcgggtat tagatgaact
      541 ggaaaccaaa gacattacag aagccttgga taaaatagtc aaacgagggg caagcgtaca
      601 tgcaaaccgc gttttaagct ctttgaagca aatgtttggc tatgcggtaa gccgtggcac
      661 tatgagcact aatccagcct ctaatattcg cgcaagagat attggcggtc atgagaaacc
      721 aagagagcgt gttttaagtc tggatgaaat caaatccctg tggttatttc tggacagtaa
      781 agactctcaa atggcaccac aaacccgtat tgctattaag attattttac taactggtgt
      841 tcgtactgct gaactgcgct tggcgcaatg gagcgaaatc aactttgatg actccctttg
      901 gacaataccg gctatgcatt ccaaggctag tattgttcat aaagtacatc ttagcgattt
      961 gaccaaacag ttattgcagc aactcaaatc cattagtcga tcagattatg tcttaacagg
     1021 tgcagattat cgccaaccat tgacagaaaa tgctttgcca agggctatta aacgcattca
     1081 agagcgttta ggtattccag aatggacagc ccatgatcta cgccgtactt ttgccaccca
     1141 actaggagag acgctccaag ttgatccagt ggtaattgag aaatgccttg gccataaaat
     1201 gccacggatt atggcaactt ataaccgcaa tgaaatgtta cctcagagac gagaagctct
     1261 cgagtgttgg gcggtatgta ttgaaaatat gatgcaagat aatgtggtat atattaacaa
     1321 actactccaa aacgaaagat ctttgtaaaa caaatttgtc atttttat
//
LOCUS       DBNCWK010000256         1305 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.257, whole genome shotgun sequence.
ACCESSION   DBNCWK010000256 DBNCWK010000000
VERSION     DBNCWK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.257"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..246
                     /locus_tag="AC06IO_002904"
     CDS             <1..246
                     /locus_tag="AC06IO_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIO9876059.1"
                     /translation="YLNRATVARANPLNANLYIYKERSKGRIKKTLQGVKCQSGNSKK
                     YAKGAKEPWIIITSLPKGLNTAKKIIKIYKLTSARHK"
     gene            complement(229..1122)
                     /locus_tag="AC06IO_002905"
     CDS             complement(229..1122)
                     /locus_tag="AC06IO_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058529992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIO9876060.1"
                     /translation="MLAPIVEIFCDIDDFCKAWFKEMSPYLLPSPNRKRQRPCRLSAS
                     EIMTVMILFHLSHYRTFKDYYRECVEMDMKSYFPKLVSYNRFVEIMSSVVTPLSAYLL
                     SHMGKKTGLYYVDSTTLKVCHNKRIHRHKTFAGIAERGKSSMGWFFGFKLHLVINHLG
                     ELIAFCITKGNVDDRQPLEQLFKNLHGLAAADKGYLSKKKEESLFQKGVKLITKVRRN
                     MKAKILSTFEKYFLGQRSIVETVIEQLKSICQIEHTRHRKPDNFVINLLSGLAAYMLK
                     PRKPSLKIHSLNKNLGLLMSS"
BASE COUNT          424 a          239 c          237 g          405 t
ORIGIN      
        1 tacctaaatc gggcgaccgt tgccagagca aatccactta atgcaaacct ttatatctat
       61 aaggaacgta gtaaaggtcg tataaaaaag acgcttcaag gagtaaagtg tcaatcaggc
      121 aatagtaaga aatatgctaa aggagctaag gagccatgga taattattac gtctcttcca
      181 aaaggcttaa atactgctaa aaagataatc aaaatatata aattaacctc agctcgacat
      241 aagtaagcct aaatttttat taagggaatg gattttgagg gaaggttttc ttggtttgag
      301 catataagca gctaagccgg ataaaaggtt aatgacaaaa ttatctggtt tacgatgtct
      361 ggtatgttca atttggcaaa ttgattttaa ttgctcaatt actgtttcaa cgatactgcg
      421 ttgtccgagg aaatattttt caaaagttga gagtatttta gccttcatgt ttctgcgtac
      481 ctttgtgatg agcttaactc ctttctggaa aaggctttcc tcttttttct ttgaaagata
      541 ccccttatca gctgcggcaa gaccatggag gtttttgaaa agctgctcta gtggttgacg
      601 gtcatccaca ttgccttttg ttatgcaaaa agcaattaat tcaccaagat gattaatcac
      661 aagatggagc ttaaaaccga aaaaccagcc cattgaggat ttgccacgtt ccgctatgcc
      721 agcaaatgtt ttatggcgat gaatacgctt gttatgacat actttcaggg tggttgaatc
      781 aacataatac agccctgtct tcttccccat atgcgacagc aaataggctg ataaaggcgt
      841 cactaccgat gacatgattt ctacgaaccg attatagctg acaagtttcg gaaaatacga
      901 tttcatatcc atctccacgc attcacgata atagtcttta aaagtgcgat aatggctgag
      961 atgaaataaa atcatgaccg tcatgatttc actggctgac agcctgcaag ggcgttgtct
     1021 tttgcgattc ggtgatggaa gtaaataagg cgacatttct ttaaaccatg ccttgcaaaa
     1081 atcatcaata tcacagaata tttctactat cggtgctaac atcttgtttc ctttcataat
     1141 tcaggcgtga attttaaaaa aacaaaatgt atatagcacc gctaattttt tcaataattc
     1201 aatcacataa ccttattctt atatcgagct gaggttattt tatctcaaat agtatctaca
     1261 atttttctca atcaaaacct aaaaatataa ttattcaaaa tgtgg
//
LOCUS       DBNCWK010000257         1287 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.258, whole genome shotgun sequence.
ACCESSION   DBNCWK010000257 DBNCWK010000000
VERSION     DBNCWK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.258"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            104..>1287
                     /locus_tag="AC06IO_002906"
     rRNA            104..>1287
                     /locus_tag="AC06IO_002906"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          381 a          241 c          408 g          257 t
ORIGIN      
        1 attctggtat agccggagtt cattaacaag atggtaaaga agaagaggta acacaagcga
       61 ttggtatatg catcatgtga ttttgaggtg attgagatta tatggtcaag aagagaagcg
      121 caaacggtgg atgccttggc agtaagaggc gaagaaggac gtggaatcct gcgaaaagct
      181 atggggagct ggaaacgagc gttgagccat agatgtccga atgggggaac ccggctgcag
      241 cgatgcggtc atttgcattt gaatacatag gatgcaaagg cgaactcggg gaactgaaac
      301 atctaagtac ccgaaggaaa agaaatcaag agagattctc caagtagcgg cgagcgaacg
      361 gggaggagcc tggcgtgatt tattattgaa ctgagtagaa caatttggga aagttggcga
      421 tagagggtga aagccccgta tacgaaggtt tgatgaggaa ctaggcacgc gaacaagtag
      481 gccgggacac gtgaaatcct ggttgaagat gggtggacca tcatccaagg ctaaatacta
      541 cttactgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagaacc ccggagaggg
      601 gagtgaaata gaatctgaaa ccgtttgcgt acaagcagtg ggagcatggt ttaggctgtg
      661 tgactgcgta ccttttgtat aatgggtcag cgagttactt tcagtggcga ggttaactgc
      721 ataagggagc cgtagagaaa tcgagtctga atagggcgat agtcgctggg agtagacccg
      781 aaaccgggcg atctagccat gtgcaggatg aaggttgggt aacaccaact ggaggtccga
      841 accgggtaat gttgaaaaat tatcggatga cgtgtggcta ggagtgaaag gctaatcaag
      901 cccggagata gctggttctc cccgaaagct atttaggtag cgcctcgtga atgattactg
      961 ggggtagagc actgtttcgg ctagggggct gtcatggctt accaaaccga tgcaaactcc
     1021 gaataccggc taattgaatc acgggagaca cacggcgggt gctaacgtcc gtcgtggaga
     1081 gggaaacaac ccagaccgcc agctaaggtc cccaagtact agttaagtgg gaaacgatgt
     1141 gggaaggcat agacagccag gaggttggct tagaagcagc caccctttaa agaaagcgta
     1201 atagctcact ggtcgagtcg gcctgcgcgg aagatgtaac ggggctaaaa ctagtcaccg
     1261 aagctgcgga tgtgcgcgaa ggcgcac
//
LOCUS       DBNCWK010000258         1247 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.259, whole genome shotgun sequence.
ACCESSION   DBNCWK010000258 DBNCWK010000000
VERSION     DBNCWK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.259"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            79..1176
                     /gene="lem23"
                     /locus_tag="AC06IO_002907"
     CDS             79..1176
                     /gene="lem23"
                     /locus_tag="AC06IO_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem23"
                     /protein_id="HIO9876061.1"
                     /translation="MKKKDEQNNFTKGKENEHNTISTNTNLLFSKPPGINVETSNHMA
                     SSIDRLKDFDSSKQSLWLKYSEDIINEFCTLLPEFPRIKPKVREDIRCPEEALLTQVG
                     CAYAFLASKRREVAKLLKHGPLRHQFGLFNKPGFASIDDFNLSKDIPSQKDKIYEHLS
                     LLINQPNPFIEVKKNNREAEGFSIYTITFKMKPDEIEQVHKKNLDSNKPFIENDTVLT
                     ITFSDYGKNDNRTSIVIDHRPTPFAENYTSESSYFKVQFELIEPYFQACQAWEPSDGI
                     NHFLENAGRIAYILARMQPVGRGNSAIVEWIIRGLAQTKNIELGPFNPDEKIGWDFKA
                     FLTPEIQDYAHWFSEKAFAHSQLKNVTSLGQ"
BASE COUNT          433 a          225 c          226 g          363 t
ORIGIN      
        1 aaacacatag atttattaat aagcaaaatc aatatggctg gctatactta agtaatgcat
       61 tatagttttt ggggtattat gaaaaagaaa gacgagcaaa ataactttac caagggtaag
      121 gagaatgagc acaataccat cagcaccaat accaatttgt tattttcaaa accacctggt
      181 ataaatgttg aaacttcaaa tcatatggct tcatctattg accgcttaaa agattttgac
      241 agttcaaaac aaagcctttg gttaaaatac agtgaagaca tcatcaacga attctgtact
      301 ttattacctg agtttcccag gataaaaccc aaagtgcgtg aagacattcg ttgcccggaa
      361 gaagcattat taacccaagt cggttgtgct tatgcttttt tagcgagtaa aagacgcgag
      421 gtagccaagc ttttaaaaca tggtcctcta agacaccaat ttggtttgtt taataagcca
      481 gggtttgcct caatcgatga tttcaacctc agtaaagata tcccctctca aaaggataaa
      541 atttatgagc acctctcctt gttaattaat caacctaatc cttttattga agttaagaaa
      601 aataatagag aagctgaagg tttttctatt tataccataa cgttcaagat gaagcctgat
      661 gaaatagaac aagtgcacaa aaaaaatttg gattcaaaca aaccatttat tgaaaatgat
      721 acggtgctga cgataacctt cagtgattat gggaaaaatg acaacagaac ctcaattgtc
      781 attgatcacc gccctactcc ttttgctgaa aactatacga gcgagtccag ctatttcaaa
      841 gtgcaatttg aattgattga gccttacttt caagcatgcc aggcctggga accctctgat
      901 ggaattaatc attttttaga aaatgctgga agaattgctt atatactggc tcgtatgcaa
      961 cctgttggac gtggtaattc tgcgattgtt gaatggataa ttagaggcct tgcccaaacc
     1021 aaaaatattg aattagggcc attcaaccct gatgaaaaaa ttgggtggga ttttaaagcg
     1081 tttctgacgc ctgaaattca ggattatgcc cattggtttt ccgagaaagc ctttgcgcat
     1141 agccaattaa aaaatgttac ttccctggga caatgaatta aactgagtaa acccgcctat
     1201 gcggtaaaaa attatacaat tgtaatagat aaattaaaga taatcct
//
LOCUS       DBNCWK010000259         1235 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.260, whole genome shotgun sequence.
ACCESSION   DBNCWK010000259 DBNCWK010000000
VERSION     DBNCWK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.260"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..>1235
                     /gene="vpdA"
                     /locus_tag="AC06IO_002908"
     CDS             <1..>1235
                     /gene="vpdA"
                     /locus_tag="AC06IO_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector VdpA"
                     /protein_id="HIO9876062.1"
                     /translation="SSAKLMRQAANYVILKKVMDIISDEKFKTRFAKFQNFLEENIYR
                     CPENITFQTLARIKEICPECELGEKLFITGTNLSTQKHEVFSIDTTPSMALADAIIIS
                     ANLPIAFERICYQGNVYSDGGISNNLPAHCFSEKGHKTTFLKHKDDVDFSVLALQFDN
                     GLEENALYSQNPIPKWSWLSNTFYSLITGHPNVTENWHEDLQILRRHAHQSILIKTPT
                     IALTNLTISQDTKKALVESGRTAAKTYLELHEFYTDDYGNIRHHECLHEKFQKPEELL
                     DYCVLHSHFELLKKIKQAISCSQYLEKGYKHYLCELCDNLLPPQLKCPNEDSETEQPE
                     IKLEKDTILCEKNNNSGLTFSMTFFGVPSPLVKTLNKDSPELKIKLFTGLYPILIQNW
                     QNLCPVSGISGILNSIRMS"
BASE COUNT          449 a          237 c          199 g          350 t
ORIGIN      
        1 tagctcggca aaactaatgc gtcaagcagc aaattacgtt attttaaaaa aagtaatgga
       61 tattatatcc gatgaaaaat tcaaaacacg atttgcaaaa tttcaaaatt ttttagaaga
      121 aaatatatac cgctgccccg agaatataac atttcaaacc ttagccagaa taaaagaaat
      181 atgtcctgaa tgtgaattag gagaaaaact atttattact ggaacaaatt tgagtaccca
      241 aaaacatgaa gtgttttcca ttgatacaac accatctatg gcactcgccg atgctattat
      301 tatttctgca aatttaccta ttgcctttga gcgtatctgc tatcaaggaa acgtatacag
      361 cgatggagga atcagcaata atttacctgc ccattgtttt tcagaaaaag ggcataaaac
      421 aacctttttg aaacacaagg atgatgttga cttcagtgta cttgctctgc agtttgataa
      481 tgggcttgaa gagaacgctc tttatagcca gaacccaata cctaaatggt catggctaag
      541 caacacattc tactcattga ttacaggaca tccaaatgtt actgaaaact ggcatgagga
      601 tcttcaaata ctgaggcgac atgcccatca aagtatttta attaaaacac caactattgc
      661 tttgaccaat ctcacaatta gtcaagatac aaaaaaagcc ctggtcgaat caggacgaac
      721 agctgccaag acctatctgg aactccatga gttttatacg gatgattatg gaaatatcag
      781 acatcacgaa tgcttgcatg aaaaatttca aaaacccgaa gagttattgg attattgtgt
      841 cctacacagc cactttgaac ttttaaaaaa aattaagcaa gccatttcct gctcacaata
      901 tttagaaaaa ggatataaac attatttatg tgagttgtgt gacaacttgc tcccacctca
      961 actgaaatgc cccaacgaag attcagaaac agaacaacct gaaatcaagt tagaaaaaga
     1021 taccatcctt tgcgaaaaga ataataatag tgggctgaca ttttcaatga cattttttgg
     1081 ggttccatcc cctttagtca aaacattaaa taaggatagc cccgaattaa aaattaaact
     1141 atttacaggt ttatatccta tactaattca aaactggcaa aatttatgcc ccgtttccgg
     1201 cattagtggt atattaaatt ctatccgcat gtcat
//
LOCUS       DBNCWK010000260         1157 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.261, whole genome shotgun sequence.
ACCESSION   DBNCWK010000260 DBNCWK010000000
VERSION     DBNCWK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.261"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..1060
                     /locus_tag="AC06IO_002909"
     CDS             <1..1060
                     /locus_tag="AC06IO_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HIO9876063.1"
                     /translation="NTVLLDTTNLYWNQLHEYATLLNQCRTTLQKSHRLTEEGQLALR
                     CFNNAIQKMPSKKHIINALKILNKSNNKTEVIEKIGFVSPHGREACMQLTCWGKKFFY
                     IPRSEEMYHFDYDPKEDLNDTGGNCFGESMMFIHFLSKGAIRWLCPEAGLINFQLDQT
                     RNLKLKKTALGEGETVVSDNSIHDSLQWKDMKPVLLDNPYFKPGSLCGVIFSMNDYTK
                     AQREFTAGHIIVVAKLDTNLNPYKYIVYEKDFGAFGLTDDESLEYLISEQILPLYSGM
                     NYSKIKLVKYGDASTETYDLLNEIKPQTSIPQKESIASDSINTYFDNAFFKLYKETNK
                     ENAINQVNETDVFGVCRN"
BASE COUNT          415 a          167 c          213 g          362 t
ORIGIN      
        1 taacactgtt ttactggata ctaccaatct gtattggaac caattacacg aatacgcaac
       61 attattaaat cagtgtcgca ctactttgca aaaatctcat cgacttaccg aagaaggaca
      121 attggcgctt cgttgtttta ataatgcaat tcagaaaatg ccatccaaga aacatattat
      181 taatgcgtta aaaatattga ataagtcaaa taacaagaca gaagtaattg aaaaaatagg
      241 ctttgtttca cctcatggta gagaagcctg tatgcaacta acttgttggg ggaaaaaatt
      301 tttttacata cctcgtagcg aggaaatgta tcattttgat tatgatccta aagaggattt
      361 gaatgatact ggcggtaatt gttttggtga gtcaatgatg tttattcatt ttttatctaa
      421 gggggcaatt agatggttat gtcctgaagc aggactaatt aattttcagt tagatcaaac
      481 aagaaattta aaattaaaaa aaacagcttt aggtgaaggg gaaacggtgg tatccgataa
      541 tagcattcat gattctttac aatggaagga tatgaagcct gtgcttttag ataatcctta
      601 ctttaaacca ggcagcctgt gcggtgtcat tttctcgatg aatgattaca caaaagcaca
      661 aagagagttt accgcagggc acattatagt agttgcaaag ctggatacta acttgaatcc
      721 gtacaagtac attgtatatg aaaaagattt tggtgcattt ggattaactg atgatgaatc
      781 tttggaatac ctaattagtg aacaaatatt accactctat agtgggatga attattcaaa
      841 aatcaaattg gttaaatatg gtgatgcttc aactgagacc tatgatcttt taaatgaaat
      901 taagcctcag acaagtattc cacaaaaaga gtcgatagcc agtgattcta taaataccta
      961 ttttgataat gcatttttta aattatataa ggaaactaat aaagagaatg caattaatca
     1021 agtcaatgaa acagatgtgt ttggggtttg cagaaactaa atcaaaatgt tgctgcttgc
     1081 ttttaaggaa ctcaaaaaac tgtgaaacaa tgagagagct atgcttaatt ccaagtcaat
     1141 atctattttc taattta
//
LOCUS       DBNCWK010000261         1135 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.262, whole genome shotgun sequence.
ACCESSION   DBNCWK010000261 DBNCWK010000000
VERSION     DBNCWK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.262"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..198)
                     /locus_tag="AC06IO_002910"
     CDS             complement(<1..198)
                     /locus_tag="AC06IO_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIO9876064.1"
                     /translation="MSKLFIAGIGIKFLSHMTLEVQSVIQKCDCVIYLVNEPAIKRWI
                     DENSKKAISLDAIYFSFQERKE"
     gene            complement(201..398)
                     /locus_tag="AC06IO_002911"
     CDS             complement(201..398)
                     /locus_tag="AC06IO_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876065.1"
                     /translation="MNTYEKLVYLATEPIDNQIVLCDKKGSKLKSSLAVQEELKDEFI
                     HADLSIDIRRACETHLSFVLR"
     gene            complement(418..1113)
                     /locus_tag="AC06IO_002912"
     CDS             complement(418..1113)
                     /locus_tag="AC06IO_002912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9876066.1"
                     /translation="MICLLVLVGIGTLLFYQDSIFNPPKLGSLSFKIILPERKQEFML
                     LMSMVLFFGLIFSMVKVVFAPTLIERFGSNMMSVIAASINPWIVFLIQPLIVGRIKNS
                     NSIWFLGIGGLIVGLSYFMFGMVNSFELTAIVLVLLTFGEMMFGPLSKHFNIQLYRSG
                     QEGVAAGIWKAVFLGSGAIGPSLSGYVAEYYGTYVVWDCCALLGVLCFVFSLYLRKIQ
                     IKMPYSKLVLDKQ"
BASE COUNT          419 a          229 c          125 g          362 t
ORIGIN      
        1 ttcttttctt tcttgaaaac taaagtatat tgcatctaat gagatagcct ttttactatt
       61 ttcatctatc catcttttta tcgctggttc attaaccaaa taaataacac agtcacattt
      121 ttgtatgaca gactgaacct ctaaggtcat atgagataaa aattttattc ctattccagc
      181 gataaacaat ttactcaaat ttacctcaat acaaaagata aatgagtttc acaagctcgc
      241 ctgatatcta ttgataaatc tgcatgaata aattcatctt ttaattcctc ctgtacagcc
      301 aacgaggatt ttaacttaga tcctttttta tcacataaaa ctatttgatt atcaatgggt
      361 tcagtagcta aatagactaa tttttcatag gtattcatat acactcactt acaatagtta
      421 ttgtttatcc aaaaccaatt tgctatatgg catctttatt tgtattttac gcaaatacaa
      481 tgaaaataca aaacaaagca cccctaataa agcacaacaa tcccatacca catatgttcc
      541 ataatattca gcgacatacc ctgacaagct aggacctata gctccacttc ctaaaaatac
      601 tgctttccat ataccagcgg caacaccctc ttgtccagat ctatataact gaatattaaa
      661 atgcttagac aatgggccaa acatcatttc gccaaatgtt aataaaacca atacaatagc
      721 tgttaattca aatgaattca ccataccaaa cataaaataa cttagaccta caattaaccc
      781 cccaatccct aaaaaccata ttgaatttga atttttaatt ctccctacta ttaatggttg
      841 tattaaaaat acaatccatg gattaattga tgcggctatt actgacatca tattgctgcc
      901 aaagcgttcg attaaggttg gggcaaaaac cacttttacc attgaaaaaa taagaccaaa
      961 aaacaaaacc atgctcatta aaagcataaa ttcttgttta cgctcaggta atataatttt
     1021 aaaactcaaa gatcctaatt taggtggatt gaaaattgaa tcttgataaa aaagcaatgt
     1081 tcctatccca actaatacta gcaaacaaat catgatctgc acgtatttca atata
//
LOCUS       DBNCWK010000262         1096 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.263, whole genome shotgun sequence.
ACCESSION   DBNCWK010000262 DBNCWK010000000
VERSION     DBNCWK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.263"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            40..1017
                     /locus_tag="AC06IO_002913"
     CDS             40..1017
                     /locus_tag="AC06IO_002913"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIO9876067.1"
                     /translation="MNLKKAELHVHLEGTISPELAKKLAKRNQLSLPNGLIAPDEKSY
                     LSRDFLDFLKVYDTLASVIKNPQDYYDITFDYLKSNAQEHAIYIEMMYSPDHAEQSSG
                     IPSKEHLAAIQQAIDDAKSQFDIVGRIIITAVRHFGVEACERVAKQASIDKFPCVTGF
                     GLGGDEAKFPPQLFTKAYQIAADSGLECTVHAGEFDSAKGMEEAMKTLPIKRIGHGVR
                     VIDSPDIMAMVKDQGIALEVCPTSNIFLGLFKDMNSHPFPKLYEAGIKVSINSDDPPF
                     MSTTLAQEYKRVQKAYAYNNDTMNNITRMAIEAAFVDEKTRKELLTKLE"
BASE COUNT          370 a          201 c          203 g          322 t
ORIGIN      
        1 accattaatc ttctttcaag taagataata ggctatatca tgaatttaaa aaaggcagaa
       61 ttacacgtac atctagaagg aactatttcc ccagaactcg ccaaaaaact agcaaaaaga
      121 aatcaattaa gtctccctaa tggtcttatt gcaccagatg aaaaaagtta cttatcaaga
      181 gactttctcg attttcttaa ggtttatgat acgctggcat ccgtaattaa aaatccacaa
      241 gattattatg atattacctt tgattatctt aaatcaaacg ctcaagaaca tgctatctac
      301 attgaaatga tgtactctcc ggaccatgcc gagcaatcca gtggaattcc ttcaaaagag
      361 catttagcgg caattcaaca agcaattgat gatgctaaaa gtcaatttga tattgttgga
      421 cgtattatta ttactgctgt acgccatttt ggcgtagaag catgcgaacg cgttgcaaag
      481 caagcttcaa tagataagtt tccttgtgta acgggatttg gactaggtgg ggatgaggca
      541 aaatttcctc ctcaattatt taccaaagct tatcagatag cagcagactc cggattggaa
      601 tgcaccgttc atgccggcga atttgattca gccaaaggaa tggaagaagc aatgaaaact
      661 ctgccaatta aaagaattgg acatggggtt cgagtgattg actcacctga cattatggcc
      721 atggttaaag accagggtat tgcattagaa gtgtgcccaa ccagtaacat atttttggga
      781 ttattcaaag acatgaatag ccaccctttt ccaaagcttt atgaagcagg gattaaagtc
      841 agtattaatt ccgatgatcc tccttttatg agtactacac ttgctcaaga gtacaaaaga
      901 gttcaaaaag cttatgctta taacaatgac acaatgaata atattacgcg tatggctatt
      961 gaagctgctt ttgttgatga aaaaacccgg aaggaacttt taacaaaatt agaataataa
     1021 tctcagtctt atattcctgt tgacagtact aattccctgc tgatttgagg cgataacgat
     1081 actgttatat ttttac
//
LOCUS       DBNCWK010000263         1092 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.264, whole genome shotgun sequence.
ACCESSION   DBNCWK010000263 DBNCWK010000000
VERSION     DBNCWK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.264"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..368)
                     /locus_tag="AC06IO_002914"
     CDS             complement(<1..368)
                     /locus_tag="AC06IO_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HIO9876068.1"
                     /translation="MAINRVTRQKFSLLINKAWFLPLMISIAINLTGITLILLSKNIN
                     GFYLSISTESQQTIISITSGSLMTLIGLTFSISMLILSTVSNQFGPRLLPNFLESRIT
                     QTTLGFFLGTFVFSLYCIYF"
     gene            complement(563..982)
                     /locus_tag="AC06IO_002915"
     CDS             complement(563..982)
                     /locus_tag="AC06IO_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIO9876069.1"
                     /translation="MNFGTWYFPSLAALFLYGAWGYWGTRAANFINPLSITFYSSIGV
                     LISGIIALILLGFKPELSVKGSTYGLLNGLANGIACIFFILALRNGPTMPVVLVTSMY
                     PMITLIFCMIFLKQELSLKQGLGMVFALIALVLFSTE"
BASE COUNT          392 a          174 c          189 g          337 t
ORIGIN      
        1 ttgaaataaa tacaataaag actaaaaaca aaagtgccta aaaaaaagcc taaagttgtc
       61 tgagtaattc ttgattccag gaaattgggt agtaagcgtg gaccaaattg gtttgatacc
      121 gtagatagaa tcagcatgct gattgagaaa gtcagaccaa ttaaagtcat taaagagcct
      181 gaagtgatgc tgataatcgt ttgttgactc tctgtagata ttgagagata gaatccatta
      241 atatttttac tcagtaaaat cagggtaata ccggttagat taatagctat tgatatcatt
      301 aaaggcaaaa accatgcttt gttaattagt aaagaaaatt tttgcctggt cactctattt
      361 attgccacgc ttgcttgtcc ttacttaatt attattaatg atatgatggt ttatcttaca
      421 gtttagttga attcaagatt ttttgaacaa actatcaatt tgagagtcaa gcttcgtgac
      481 caaaattttt aatttttaga gcaaggctct ttaattttga taatccaaag aaatttatgg
      541 ctatttcatc tgattggagt tttcattccg ttgaaaataa aactaaggca atcagtgcga
      601 aaaccatccc caatccttgt tttaaactta attcttgttt gagaaaaatc atgcagaata
      661 ttaaagtaat catgggatac atagaggtga ccaaaactac tggcatggtg gggccattac
      721 gtaatgccaa aataaaaaaa atacaggcaa ttccattagc cagcccattg agcaagccat
      781 aagtactccc tttaacagaa agttcgggtt tgaaacccaa taaaatcaaa gcaatgattc
      841 cagaaattaa aacccctata cttgaataaa aggtgataga aaggggatta ataaaattag
      901 cggctcttgt cccccaatat ccccatgcac cataaagaaa taatgcagcc aaagaaggaa
      961 agtaccaagt accaaaattc ataattttat cttatcaagc aatttgtttg aggtttgctc
     1021 aggataactg ttcattggct aaaataatac tgtttattgc taaattttgc ttattatcac
     1081 gatgaggctg cg
//
LOCUS       DBNCWK010000264         1065 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.265, whole genome shotgun sequence.
ACCESSION   DBNCWK010000264 DBNCWK010000000
VERSION     DBNCWK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.265"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(81..1055)
                     /locus_tag="AC06IO_002916"
     CDS             complement(81..1055)
                     /locus_tag="AC06IO_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1137 family Dot/Icm T4SS effector"
                     /protein_id="HIO9876070.1"
                     /translation="MIQRGFTMQERREKQGNSSPYLLTPYEMANLVFKTGAISFTVSA
                     GTQPFQYLLNKLQFSQSGTPSGLSGGLFRGMYRGFLPYAIAGQKRGAVAVTHKQTNKA
                     TEEEEFEAPFRQRWLGTIFFSQADLLVSNGLSGKARLQNVGVINAENFKWSLSNFWKL
                     TSVNWGSRSFAGGVNFALIGFAGDYVSSFYKFDKDLYNKILGGATSGVIATLFTTVPN
                     AYADSKLLQTKVAENNRLVTVSPYTMFGQMKSHVKAVGLKDAFMTFFKVNYLQQVAVR
                     APQAAITFALIFGMDEYMGPQPLKKIWPGRVEIEELESETPSPSPTKK"
BASE COUNT          329 a          216 c          200 g          320 t
ORIGIN      
        1 ataagtacaa tttataccgt aaaacgtatt gttatttgtt aaggcacaat gatgaaaaat
       61 aacatccttg ttactcttaa ttatttcttg gtaggggatg gggatggagt ctctgactca
      121 agctcttcga tttcgactcg tccaggccaa atctttttca aaggttgagg acccatatat
      181 tcatccatac caaaaatgag ggcaaaagtg atggctgctt gaggagctct taccgctacc
      241 tgttgcaaat agttcacttt aaaaaaggtc atgaacgcat ctttaagacc aaccgctttt
      301 acatgtgatt tcatttgacc aaacatggta tagggtgaca cagtaactaa acggttattt
      361 tcagccactt tggtttgcaa caatttacta tccgcataag cattagggac ggttgtaaat
      421 aatgttgcaa tgacaccaga agtagcgccg cctaatattt tgttatacaa gtctttatca
      481 aacttataaa atgaactgac atagtctcct gcaaatccta tcaatgcgaa attgacccca
      541 ccggcaaaag aacgagaacc ccagttgact gaagttaatt tccaaaagtt ggaaagggac
      601 catttaaagt tttcagcatt aataacgccg acattttgca atcttgcttt tccacttaaa
      661 ccattactaa ccaatagatc agcctgagaa aagaatatgg ttcccaacca acgttgacgg
      721 aatggggctt cgaattcttc ctcttctgtt gctttgttag tttgtttatg ggttacagcc
      781 acagcaccgc gtttttgacc tgcaatagca taaggaagga atccacggta catgcctcga
      841 aaaagtcctc cgctcaatcc agatggagtt ccagattgtg aaaattgcaa tttgtttagc
      901 aagtattgaa atggttgagt tcctgcagaa acagtgaaag aaatagcccc tgttttaaaa
      961 actaaattgg ccatttcata gggcgtcaac agatagggtg aactattacc ctgtttctct
     1021 ctacgttctt gcattgtaaa tcctctttga atcacttaaa tcaat
//
LOCUS       DBNCWK010000265         1036 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.266, whole genome shotgun sequence.
ACCESSION   DBNCWK010000265 DBNCWK010000000
VERSION     DBNCWK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.266"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..>1036
                     /gene="lbtU"
                     /locus_tag="AC06IO_002917"
     CDS             <1..>1036
                     /gene="lbtU"
                     /locus_tag="AC06IO_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072401887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="siderophore legiobactin import outer membrane
                     protein LbtU"
                     /protein_id="HIO9876071.1"
                     /translation="GILMLNVTGLAFADAGDSLFKNEKLTYDGHLFFNAANSTIQGEK
                     YLLNQQLSNIKMGSELQVTDWNKMKGLLIYNTLPTPVAPQFYFEQFYDELQIESSNIF
                     LAFGKKWLTFGNYKSDLIYKPLTKALGQTNEVAAIIGYDSIYYANASFFKPYSRISTS
                     SLPLYYNLNTGVHNQSMDTGVSYLYSLAESQLFQYNKGFGGLLSQSLKTRVPGFATYF
                     NLKYKKTSTYLTYVTAITPFALEDMSYNNRRASPKALSIQNSYDVNIKNFSFKITGFY
                     DYTFQALALGIPKQRLGLGLSATPFPYLGIQFQYSRDYSYGNTAIASGINHFVNGRNT
                     KMNNLAFQTIL"
BASE COUNT          354 a          190 c          163 g          329 t
ORIGIN      
        1 aggaatatta atgttgaatg ttaccggctt agcttttgca gacgctggag actctttatt
       61 taaaaatgaa aaattgacct acgatggaca tctgtttttt aatgccgcaa actcaacaat
      121 acaaggtgaa aaatatttac tgaatcagca gctatccaat atcaaaatgg ggtctgaatt
      181 acaggtgact gattggaaca aaatgaaagg tttgctaatt tataacacct tgcctacccc
      241 agtggctcct caattttatt ttgagcaatt ttatgatgaa ctccaaatag agtcttcaaa
      301 tattttcctt gcatttggaa aaaaatggct cacatttgga aattataaaa gtgatttaat
      361 ttataaacca cttacaaaag ccttgggaca aaccaacgaa gtagccgcta ttattggata
      421 cgattccata tattatgcta acgcttcttt ttttaaaccc tattctcgca taagtacatc
      481 atcacttccg ctttattaca atctgaatac aggagtacat aatcaatcaa tggatacagg
      541 agtcagttat ttatactctc tggcagaaag tcaattattt caatacaaca aaggttttgg
      601 cgggctttta tcacaatcat taaaaacaag ggttccagga tttgcaacct attttaattt
      661 aaaatataaa aaaacaagca cttatctcac ctatgttact gcaatcaccc cctttgcatt
      721 agaagacatg agttataaca atcgaagagc gtcgccaaag gcattgagta tacaaaatag
      781 ttatgatgtt aatattaaaa atttttcttt taaaatcact ggtttttatg actacacttt
      841 tcaagctctg gcacttggaa taccgaaaca aagactagga ctgggattgt ccgcaacccc
      901 ttttccttat cttggtattc aatttcagta ttccagagac tatagttatg gaaatactgc
      961 catagcatcg ggtatcaatc attttgtaaa tggccgtaat acaaaaatga ataatttggc
     1021 attccaaacc atactg
//
LOCUS       DBNCWK010000266         1033 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.267, whole genome shotgun sequence.
ACCESSION   DBNCWK010000266 DBNCWK010000000
VERSION     DBNCWK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.267"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            174..866
                     /locus_tag="AC06IO_002918"
     CDS             174..866
                     /locus_tag="AC06IO_002918"
                     /inference="COORDINATES: protein motif:HMM:NF025056.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIO9876072.1"
                     /translation="MTKCTAPVRGHSSAAAASTCPVCRYRTSYRYGGYNSPSYSSPDS
                     SRANYSSSGGRSSSSRPRWSRAGSSLSYTSAQVQSLTPIRQTLETRAAEQPDLRDVFL
                     CHAWDDRRGPAKELHDLLVEAGVKVWFSEKDLGLGVPMMRAIDKGLANSRIGLVLVTP
                     ALLARLPKESVADKELSVLLAGNQLVPIVHNTTYEALRNVSPLLASRTGFDTTEDSMA
                     VVAAKISELVAP"
BASE COUNT          261 a          263 c          236 g          273 t
ORIGIN      
        1 atataaattc cattgttacc cacaaattgc cccaagttag tctcttgatc atatctaaaa
       61 aacacattat attgttatat aatgtatcaa taatgctata tgttgtgttt ttctttatat
      121 atataaagat aatatcaaac ttatatggct attgtgactg gaggtattac gatatgacta
      181 aatgtacagc gccagtacgc gggcatagct cagcggctgc ggcctcgacc tgccctgtat
      241 gccgttaccg cactagctac cgctacggcg gctacaacag cccgtcctac tcttcaccag
      301 acagcagcag agctaactat agcagcagtg gtggcaggtc aagtagttca agaccgcgct
      361 ggtccagagc gggttcgtcc ttgtcataca cgtctgccca agtacagtca cttacaccaa
      421 tacgccaaac acttgaaaca cgcgcagcgg agcaacctga ccttcgtgac gtcttcctgt
      481 gtcacgcctg ggacgaccgc cgtggtccag ccaaagagct gcacgacttg ctcgtcgagg
      541 ctggtgtcaa agtttggttt agtgagaagg atcttggcct tggggtgccg atgatgcgcg
      601 ccatcgacaa agggttagcg aattcgcgga tcggacttgt actggtgacc cctgcactgc
      661 tggcccgtct tcccaaagag agcgtcgccg acaaagagct ttcggtactc ctggcgggca
      721 accagctcgt tcctattgta cacaacacga cgtatgaagc tctccgcaat gtcagccctt
      781 tgcttgcctc tcgaaccggc ttcgacacca cagaagattc aatggcggtg gtcgcggcaa
      841 aaatttctga gttggttgct ccctagcctt attgcccaca tagtcgcttt atctgggctt
      901 caaagtatag ggcttagtgt gtacatttta aaaaataatt ttggagaaac atttcggtaa
      961 acaaaacccc cgttttattc cctgtctaat attattggct cacgagaata cttggtagct
     1021 catttgacag ata
//
LOCUS       DBNCWK010000267         1032 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.268, whole genome shotgun sequence.
ACCESSION   DBNCWK010000267 DBNCWK010000000
VERSION     DBNCWK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.268"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            232..960
                     /locus_tag="AC06IO_002919"
     CDS             232..960
                     /locus_tag="AC06IO_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876073.1"
                     /translation="MRYFYEKNARIHSTNSIDELISHSFPIDVKSPEKSIHYQAVANI
                     IFSSQYKLSLLTTREDILLGQDFIDFIAHHKLTSLNKYNGVNLLYLLALSHDTALRAG
                     IWKLGSLPHLKATEIISSLSKLDAIDRMPLMKEALVAWKELFLKAKFELQQEAGTCPD
                     STRNMLGQMTLYVSTNNDKNNERDGQSQSDQTRLEVEREQLRLLERAYYKEKDRHHQP
                     TEMQPYYSDEEEEYNVTQQQYYYN"
BASE COUNT          360 a          170 c          174 g          328 t
ORIGIN      
        1 taggtttttg caaatcttcc tctcctatgt cctgtggcta gtccggtgtt gaaacctttt
       61 ttgagaaacc ggcatgtgct taatcatata tcatacatta agaatataaa aacattatat
      121 aacatatata taaatgtttt attttaactc catctgttta taaattataa ttattgggca
      181 aattaaatat ttatgttgaa tttcactcca actttttaaa aaggaaaata aatgcgttat
      241 ttctatgaaa aaaatgcccg gatccattcg acaaattcta ttgatgaatt gatttctcat
      301 tcatttccca ttgatgtaaa atcaccggaa aaaagtattc attatcaggc tgttgctaat
      361 attatttttt cttcgcaata caagttatct cttttaacta cgagagaaga catactctta
      421 gggcaagatt ttatagattt catagcgcat cataagttaa cttcgttaaa caagtataat
      481 ggggttaatc ttttatatct tttagcctta agccatgata cggctttgcg tgctggaatt
      541 tggaagttag gctctctgcc acatctaaag gctactgaaa ttatcagtag cctatcaaaa
      601 ttagatgcta tcgatcgcat gcctcttatg aaagaagctc tagtagcatg gaaagagtta
      661 tttctaaaag ccaaatttga attgcaacaa gaagcaggga cttgcccaga tagcacccga
      721 aatatgttgg gtcagatgac tttatacgtt tctacaaata atgacaaaaa taatgaacgc
      781 gatggacagt ctcagagcga tcaaacaaga ttagaagttg aacgtgagca acttcgtttg
      841 ctggaaagag cttattacaa agaaaaggat agacatcatc agcctacaga aatgcaaccg
      901 tattacagcg atgaagaaga ggaatataat gttacacaac agcagtatta ctacaattaa
      961 taattgtaat aaggaaggat ttaaagtttc ctctaatgac tgcttcacac gatatctaat
     1021 gcaatgaaat ag
//
LOCUS       DBNCWK010000268         1028 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.269, whole genome shotgun sequence.
ACCESSION   DBNCWK010000268 DBNCWK010000000
VERSION     DBNCWK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.269"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            24..884
                     /locus_tag="AC06IO_002920"
     CDS             24..884
                     /locus_tag="AC06IO_002920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIO9876074.1"
                     /translation="MKIKTVTRYLLLFSIFFLIGFNNVFAQKKSIAITIDDLPFVGEY
                     RNFHLNMMMNTMKDEKVPATGFIIAKEVRPDNWEILHKFRDAGFGLGNHTFSHANLNK
                     LKAKEYIQEIKEADSLLMPVLTEPKYFRYPYLAMSSGNKKNKILCYLEKHHYQVAPIT
                     IDSKDFVFNQRLLSVPELGRRAYLDELKPFYLDFIWQQTLKAEEHTQYHHNPDQAQIL
                     LIHANLLNAYVLPDIINLYKQNGYTFVSLEDALKTFKENYHCHKTRILAKKPVGEETD
                     LNIESFMDWD"
BASE COUNT          358 a          195 c          162 g          313 t
ORIGIN      
        1 acattacaca tattaaattt cgaatgaaaa tcaagactgt tacgagatac ttactacttt
       61 tctcaatatt tttcctgata ggatttaaca acgtatttgc tcaaaaaaaa tccatagcaa
      121 ttactattga tgatttgccc tttgttggtg aataccgcaa ctttcatctg aatatgatga
      181 tgaatacaat gaaggatgaa aaagtcccgg caacaggttt tatcattgcc aaagaagtaa
      241 ggcctgataa ttgggaaata ctccataaat tccgtgacgc aggtttcggc ttgggcaacc
      301 atactttttc tcatgccaat ttaaacaaac ttaaagcgaa agaatatata caggaaatta
      361 aagaagcaga ttcactttta atgcccgtcc tcactgaacc caagtatttt cgctatccct
      421 atttggcaat gagttctgga aataaaaaaa ataaaattct ctgctacctt gaaaaacatc
      481 actatcaagt cgccccgatt accattgaca gcaaagattt tgtctttaat caacgcttat
      541 tatctgtgcc tgaattagga aggagagctt acctggatga gttgaagcca ttttatctgg
      601 attttatctg gcaacaaacg ttaaaagctg aagagcatac gcaatatcac cataatccag
      661 atcaagcaca aatcctgtta atccatgcta acttacttaa tgcctatgtc ctgcctgata
      721 ttattaattt atacaagcaa aatggttaca cgtttgtgag tctggaagat gccttaaaaa
      781 cattcaaaga aaactatcat tgtcataaaa ccagaatcct tgcaaaaaaa cctgtcggag
      841 aggaaacgga tttaaacata gaatcattta tggattggga ttaataagca ccattaagtc
      901 ttcagactgt aaattttttt tccagactaa tgtttctggc ccctatataa taataacctt
      961 gctaaccaga ctatccttaa aattgcaata gctaactgga tcaagcattt gctagctaat
     1021 tatttgta
//
LOCUS       DBNCWK010000269         1013 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.270, whole genome shotgun sequence.
ACCESSION   DBNCWK010000269 DBNCWK010000000
VERSION     DBNCWK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.270"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            157..>1013
                     /locus_tag="AC06IO_002921"
     CDS             157..>1013
                     /locus_tag="AC06IO_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HIO9876075.1"
                     /translation="MLNLKKTAVAVLALGSSAVFAGTMGPVCTPGNVTVPCERTAWDI
                     GITALYLQPIYDADWGYNGFTQVGGWQHWHDVDHEWDWGFKLEGSYHFNTGNDINVNW
                     YHFDNDSDHWADFANWHNYNNKWDAVNAELGQFVDFSANKKMRFHGGVQYARIEADVN
                     RYFNNFAFNGFNSKFNGFGPRTGLDMNYVFGNGFGIYAKGAAAILVGTSDFYDGINFI
                     TGSKNAIVPELEAKLGADYTYAMAQGDLTLDVGYMWFNYFNAMHNTGVFNGFETDFAA
                     SGPYIGLKYV"
BASE COUNT          282 a          183 c          234 g          314 t
ORIGIN      
        1 ttgataagaa atttcagcaa tactgttgac tttatagaag tccggctgga taatttatcc
       61 aggtacgctc tcgattagag cgaagaatgc gtactgttgc tgatatgcta aattagtaac
      121 gaacggaaat taataataaa aaaagtggag ataagcatgt taaatttgaa aaaaacagcg
      181 gtagctgttc ttgctttagg aagcagtgca gtgttcgctg gtactatggg accagtttgc
      241 accccaggta atgtaactgt tccttgcgaa agaactgcat gggatattgg tatcaccgca
      301 ctatatttgc aaccaatcta tgatgctgat tggggctaca atggtttcac ccaagttggt
      361 ggctggcagc attggcatga tgttgaccat gagtgggatt ggggcttcaa attagaaggt
      421 tcttatcact tcaatactgg taacgacatc aatgtgaact ggtatcattt tgataacgac
      481 agtgatcact gggctgattt tgctaactgg cacaactaca acaacaagtg ggatgcagtt
      541 aatgctgaat taggtcaatt cgtagatttc agcgctaaca agaaaatgcg tttccacggc
      601 ggtgttcaat acgctcgcat tgaagctgat gtgaaccgtt atttcaataa ctttgccttt
      661 aacggattca actccaagtt caatggcttt ggtcctcgca ctggtttaga catgaactat
      721 gtatttggca atggctttgg tatttatgct aaaggagctg ctgctattct ggttggtacc
      781 agcgatttct acgatggaat caacttcatt actggttcta aaaatgccat cgttcctgag
      841 ttagaagcta agcttggtgc tgattacact tacgcaatgg ctcaaggcga tttgacttta
      901 gacgttggtt acatgtggtt taactacttc aacgctatgc acaatactgg cgtatttaat
      961 ggatttgaaa ctgatttcgc agcttctggt ccttacattg gcttgaagta tgt
//
LOCUS       DBNCWK010000270         1010 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.271, whole genome shotgun sequence.
ACCESSION   DBNCWK010000270 DBNCWK010000000
VERSION     DBNCWK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.271"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            169..990
                     /locus_tag="AC06IO_002922"
     CDS             169..990
                     /locus_tag="AC06IO_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIO9876076.1"
                     /translation="MPRFEQHLINISGKTRRKPCSMSMSEIMTIVIHYHQSNYRNFKS
                     YYLFVLQKNLHPYFPSLVSYSRMTELMSSCLVPLMAFCHHQTKTPTGIYFVDSTPIEV
                     CNVKRASQNKVFKGLAEKSKSTMGWYFGFKLHLIVNDKGELMAFKITSSRTDDRFVVP
                     NLSENLFGKMIGDKGYISQNLTDKLAERGLQLLTKARKNMKQKVLNTFDKALLRKRAI
                     VESVIDQLKNISNIEHSRHRSIFNFMVNILAGLAAYALKPKKPSLNIQRSFMAVA"
BASE COUNT          354 a          171 c          179 g          306 t
ORIGIN      
        1 tcgatataac tgtctaaaaa acaaacttaa gaatggtaac atattgaaaa accagaccat
       61 ttgtgatctt attcgttttt tcgaagaaca aaaataagaa tcaagaggtc tggagtggat
      121 aaattaatag aattattctg tgttgtcgat gatttttgcc aaaaatatat gccacgattt
      181 gagcaacatc tgataaatat ttcaggtaag accagaagaa aaccatgcag catgagcatg
      241 tcagaaatta tgactattgt aattcattat catcaatcaa attaccgaaa ttttaaaagt
      301 tactacttgt tcgtgttgca gaaaaattta cacccctatt ttccaagctt ggtgagttac
      361 agcagaatga cagaactaat gtcttcttgt ctggtaccct taatggcctt ttgccaccat
      421 caaacaaaaa cacccactgg aatttacttt gtagattcaa caccaataga ggtgtgcaat
      481 gtaaaaagag caagccagaa caaggtcttt aagggtttgg cagagaagtc caagtccaca
      541 atgggatggt atttcggctt taagcttcat ttgatagtca atgacaaagg cgaattaatg
      601 gcttttaaaa taacatcaag ccgaactgat gaccgatttg tcgttcctaa tttgagcgaa
      661 aatctgtttg gtaaaatgat tggcgataaa ggttatattt ctcaaaattt aactgacaaa
      721 ttggctgagc gaggattaca actccttact aaagccagga aaaacatgaa acaaaaagta
      781 ctcaatacat ttgataaagc tttgcttaga aaaagagcca ttgttgagtc agttatcgat
      841 caattgaaaa atatttccaa tattgaacat tcaaggcatc gctctatatt caattttatg
      901 gtcaatatat tggccggttt agctgcttat gcacttaaac caaagaagcc ctctttaaat
      961 attcaacgat cttttatggc tgttgcttga gcagttatat cgaattcacg
//
LOCUS       DBNCWK010000271         1002 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.272, whole genome shotgun sequence.
ACCESSION   DBNCWK010000271 DBNCWK010000000
VERSION     DBNCWK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.272"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            107..>1002
                     /locus_tag="AC06IO_002923"
     CDS             107..>1002
                     /locus_tag="AC06IO_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIO9876077.1"
                     /translation="MDLRKINLNLLLHLDTLLNELSVSKAAEKSFMSQTAMSHILKQL
                     RDIFDDPLFIRKPHGLQPTQKVLDLAPKIKKFLNYSNDIFEEDTFNPGNDNLFLKTAS
                     VGTGEYFILPRLCAHLAKNAPKFVLKISSLSEYLSLEYLLMSELDFAIGPGFVETGNN
                     IIRELLFEEEAVCVMHKSHPLANQELTQDMYLQAEHVDIQFSYMGNENILYKALQGYH
                     QRSIKVIVPNIISALEVVYHTNFITTVPRTLADTFKDRYQIELHPFPFPKKTFLVNFY
                     YHKRLSNHKPLQWVLDVIKKYC"
BASE COUNT          351 a          164 c          173 g          314 t
ORIGIN      
        1 ttaataaaga aaaagatatg gccggtaaat tccaaattgt agtttttggg cttattcagt
       61 taccctggat aggaatataa aaaagttaat ttggatttaa tgaaaaatgg atctcagaaa
      121 aattaatctc aacttgttat tgcatcttga tacattgtta aatgagctct cagtatccaa
      181 agcagctgag aaatctttca tgagccagac tgcaatgagc catatattaa aacaattacg
      241 tgatatattc gatgaccctc tgtttattag gaaaccgcac gggcttcaac caacacaaaa
      301 ggtacttgat ctggccccca agattaaaaa atttttaaat tactccaatg atatttttga
      361 agaagatact ttcaatccag gaaatgataa tttattttta aagactgcat cagtaggtac
      421 aggggaatat tttattttac ccaggttatg tgctcatttg gctaaaaatg ctccgaaatt
      481 tgtattaaag atttcttcac tgtcagagta cttgagtctc gagtatttat taatgtccga
      541 attagacttt gcaataggac caggctttgt tgaaacgggg aataacatca tcagagaatt
      601 attgtttgaa gaggaggccg tgtgcgtcat gcataaatca catcctctcg caaatcagga
      661 gttaactcaa gatatgtact tgcaagctga acatgttgac atacaattta gttacatggg
      721 taatgaaaat atactatata aagcgttaca aggatatcat caaagaagta tcaaagtgat
      781 agtgcccaat atcattagcg cattggaagt ggtttatcat acgaatttca ttacaactgt
      841 accgagaact ctagctgata ctttcaaaga cagatatcaa attgaactac accccttccc
      901 tttccctaaa aagacatttt tagttaattt ttactatcat aaacgattat caaatcataa
      961 accgctgcaa tgggtattgg atgtaataaa aaaatattgt tg
//
LOCUS       DBNCWK010000272          973 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.273, whole genome shotgun sequence.
ACCESSION   DBNCWK010000272 DBNCWK010000000
VERSION     DBNCWK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.273"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..531)
                     /locus_tag="AC06IO_002924"
     CDS             complement(<1..531)
                     /locus_tag="AC06IO_002924"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_component: GO:0032991 - protein-containing complex
                     [Evidence IEA];
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:1902444 - riboflavin binding [Evidence
                     IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0036001 - 'de novo' pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIO9876078.1"
                     /translation="MARNDPIALLTTWISEELERGAPNPQHATLSTTGKDSIPHGRVI
                     AIREINEQGLLFFTQRGTRKVVEMTYNPMVSLTFWFELFEREVMIDGSARPLSESENQ
                     AYWDTYPPFAQIRFHSYAPTSSHVIPSKQLIENKRKILISRFQDKSLPMSEYYCGFRV
                     IPTRMVFYTYRTDELSD"
     gene            complement(665..>973)
                     /locus_tag="AC06IO_002925"
     CDS             complement(665..>973)
                     /locus_tag="AC06IO_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIO9876079.1"
                     /translation="VEAIYGCKWSLTIYSLLRNGINRPGEMVRSVEGLSTKVLNQCLR
                     KNIEFGILGRNTFHEIPPRVEYFVTPFGEKFLKILDQIEELNEQMRDYQQIEFNNNDS
                     "
BASE COUNT          284 a          189 c          179 g          321 t
ORIGIN      
        1 atcagacaat tcatccgttc tataagtata aaaaaccatg cgtgtgggaa tgactctgaa
       61 accacaataa tattcactca taggcaatga cttatcttga aaccgagaaa tcaatatttt
      121 tcttttattt tcaatgagtt gtttactggg tataacgtgt gatgaagtag gcgcataact
      181 atgaaatctg atttgcgcga aaggcggata ggtatcccag tatgcttgat tttcgctttc
      241 cgataatggc ctggctgaac cgtctatcat gacttctctt tcaaataact caaaccaaaa
      301 agtcagagat accataggat tataggtcat ttcgacaacc tttctggttc ctcgttgggt
      361 aaaaaataat aaaccttgct cattgatttc gcgaatggcg ataacacgcc catggggaat
      421 agaatctttg cctgtcgtag ataatgtggc atgttgtgga ttaggtgctc ctctttccaa
      481 ttcctctgaa atccatgttg ttagaagggc aatggggtcg tttctggcca taacttgaat
      541 acctattgta aatattttct ggcgttaata atgatacgga ctagcgatgt ctcattacgt
      601 tggtaaatat tgtgagccat aattcacttg cagtcgatta gcaataggtc ttttataaca
      661 aatcttaact atcattattg ttgaattcga tttgttgata atccctcatt tgctcgttca
      721 attcttctat ttgatccaga atttttaaaa acttttctcc aaaaggagtc acaaagtact
      781 caactctggg gggtatctca tgaaaggtat ttctgccgag aatcccaaat tcaatattct
      841 tccgcaggca ttggtttaaa acttttgttg atagtccttc aacgcttcgt accatttcac
      901 ctggtcgatt gattccattt cgtaaaagag aatagatagt caacgaccac ttacaaccat
      961 agatagcctc tac
//
LOCUS       DBNCWK010000273          966 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.274, whole genome shotgun sequence.
ACCESSION   DBNCWK010000273 DBNCWK010000000
VERSION     DBNCWK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.274"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..872
                     /locus_tag="AC06IO_002926"
     CDS             <1..872
                     /locus_tag="AC06IO_002926"
                     /inference="COORDINATES: protein motif:HMM:NF025388.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HIO9876080.1"
                     /translation="IKKGEITENDITILKIFLYFQFIRVPIFMSMMQKSFSQVAKWVD
                     DFSRNSDASEEVIDLSKKLIIGGISKDNVILNQASLIFSNNTGQDFLTSDNPVVRKII
                     NWRQTSNLIGRDFLISTYDNSIEKPIFYMPISSNLAFISCDFLDKSGVLDRENGQIYV
                     NQLNIQMCLNADKSVYAENRSPLIDQNSFINHLDNCRIKKNGDFTKIITESDIYYLSD
                     IYDLNREKFKITFKTKNNNFLEAISKGSEIKLIEIHAQHEESGMREAKITSLNKTTGE
                     VIIESNFKPIQKL"
BASE COUNT          374 a          131 c          149 g          312 t
ORIGIN      
        1 atataaaaaa aggtgaaatt acggaaaatg atataactat tcttaagatt tttctttatt
       61 ttcaattcat cagagtacct atttttatgt caatgatgca aaaatcattt tcgcaagttg
      121 caaaatgggt agatgatttc agtagaaata gtgatgctag tgaagaagtt atagatcttt
      181 ccaaaaaatt aatcatagga ggcataagca aggataatgt gattctaaat caagcatctt
      241 taatcttttc aaataatacg ggtcaagatt tccttacttc agataaccct gtagtacgaa
      301 aaataattaa ttggagacaa acgtcaaatc taataggaag agatttttta attagtacct
      361 atgataattc gatagaaaaa cccatatttt atatgccaat ctcatctaat cttgctttta
      421 tctcctgtga ttttttagat aaaagtgggg tactggatag agaaaatggg caaatctacg
      481 ttaaccaatt aaatatccag atgtgtttga atgccgacaa atcggtatat gctgaaaatc
      541 gatctccctt gattgatcaa aattcattta taaatcattt agataattgt cggattaaaa
      601 aaaatggtga ttttacaaag atcataactg aaagtgatat ttattatctt tctgatatat
      661 atgatttgaa tagagagaag ttcaaaatta cttttaaaac aaaaaataat aatttcctcg
      721 aagcaatatc aaaaggctca gaaattaaat taatcgaaat tcatgctcaa catgaggaaa
      781 gtggaatgag agaggccaaa attacatcac taaacaaaac tactggtgaa gtaattattg
      841 agtcgaattt taaacccata cagaaattat aaattttttg ttcatgactc acctcaaact
      901 tcgacacata ttcgacacga aaaatttaag gtttagagag attttactag aagccatact
      961 gtgtat
//
LOCUS       DBNCWK010000274          941 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.275, whole genome shotgun sequence.
ACCESSION   DBNCWK010000274 DBNCWK010000000
VERSION     DBNCWK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.275"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..>941)
                     /locus_tag="AC06IO_002927"
     CDS             complement(<1..>941)
                     /locus_tag="AC06IO_002927"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIO9876081.1"
                     /translation="MDKNSKSFGEANERIGNAIHHVCLNASDAKFIKSNIQESYQSWA
                     NVLYDRVFLNWGLCDKKIYRELSSLNFDFSTVGFFPHVYGQSLLFYLIRPLIKIHFFN
                     KRLLDVGCGNGVGLKISSELLKTRYALGIDLVSKLVTNSNNSFYIEDKINYMQADAEN
                     MAIANESFDIVTNLESSHLYPQIEHFFSEVERVLAPNGFFCYADVNFDVKRQAERFEA
                     FVKTRKNLRIIEKHNITKMIQASIYQRLIVNEKGFYQLAQYLLGPDKIHYTSELANVA
                     SSMGLMFLPWWRIRFKNPALQALGKFARRCKHWNKKY"
BASE COUNT          311 a          193 c          138 g          299 t
ORIGIN      
        1 taatattttt tattccagtg cttgcaccgg cgagcaaatt ttcccagcgc ttgcaatgcc
       61 ggattcttaa aacgaatcct ccaccaggga agaaacatga gccccattga actggccaca
      121 ttagccagct ccgaggtata gtgaatctta tcaggcccca aaaggtattg agccaattgg
      181 taaaatcctt tttcattgac aattaaacgc tgatagattg atgcctggat cattttagta
      241 atattatgct tttcaataat acgtaaattt tttcttgtct tcacaaaagc ttcgaatctc
      301 tccgcttgcc gcttcacatc aaaattaacg tccgcataac aaaaaaatcc gttaggtgct
      361 aaaaccctct ctacttcaga aaaaaaatgc tcgatttgag gataaagatg tgaactttca
      421 aggttagtga caatatcaaa actttcgttt gcaatagcca tattttctgc atcagcctgc
      481 atataattaa ttttatcttc tatatagaag ctattattgg aattagtaac caatttgctg
      541 accaaatcaa tgcctaacgc gtatcttgtt tttaataatt cagaacttat tttcaaacca
      601 acaccattac cacaacccac atcaagtaat cttttattaa agaaatggat ttttattaat
      661 ggccttatca aataaaaaag aagtgattgc ccataaacat gagggaaaaa acctactgta
      721 gaaaaatcaa aattcaatga cgataattcg cgatatattt ttttatcaca taagccccag
      781 ttcaaaaaaa ctctgtcata aagcacattg gcccagctct ggtagctctc ttgaatattg
      841 gatttaatga attttgcatc cgacgcattt aaacagacat gatgtattgc attacctatt
      901 cgctcatttg cttcaccaaa gcttttggaa tttttatcca t
//
LOCUS       DBNCWK010000275          917 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.276, whole genome shotgun sequence.
ACCESSION   DBNCWK010000275 DBNCWK010000000
VERSION     DBNCWK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.276"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..632
                     /gene="pdxH"
                     /locus_tag="AC06IO_002928"
     CDS             <1..632
                     /gene="pdxH"
                     /locus_tag="AC06IO_002928"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIO9876082.1"
                     /translation="LADIRRDYGELQLSEESAENDPISQFKLWFDDVLLNEKNDPTAM
                     VLSTVDEKGYPDSRVVLLKGLENGNFIFYTNYQSAKAMQIQKNPYAALNFYWPQMARQ
                     VRVRGRVKKISSEQSDAYFSSRPLKSQFSAIVSPQSQEILDRISLEDALNQLIEEYGQ
                     KPVVRPENWGGYMIIPDEIEFWQGRDNRLHDRIHYYRHGHEWTHRRLAP"
BASE COUNT          280 a          149 c          198 g          290 t
ORIGIN      
        1 gtcttgcgga tattagacga gattatggtg agttgcagct cagtgaagaa tctgctgaaa
       61 atgatcccat ttctcaattc aaactatggt ttgatgatgt attactaaat gaaaaaaatg
      121 atcccacggc aatggttctg tctacagtag atgaaaaggg ctatccagat tccagagttg
      181 tcttattaaa aggattagag aacggtaatt ttatctttta taccaattat caaagtgcta
      241 aggcaatgca aatccaaaag aatccttatg ccgcacttaa tttttattgg cctcaaatgg
      301 caagacaagt acgtgttcgt ggtcgagtaa aaaaaatcag tagcgagcaa tccgatgctt
      361 atttctcctc cagaccttta aaaagtcagt ttagcgcgat tgtttctccc caaagccaag
      421 aaattctgga taggatttct ttagaagatg ctttaaatca gttgattgaa gaatatggcc
      481 aaaaacccgt agtcagaccg gaaaactggg gtggatatat gataatcccg gatgagattg
      541 agttttggca gggtagagat aacagattac atgatcgaat tcattattac cggcatggtc
      601 atgaatggac gcaccgtaga ttggcacctt gaccggcagt ttttgtcatc attcctgccg
      661 gcatcatttt actattgaga tatagaagga tgagttttca tggaaggttg gttaatggtt
      721 gaattctttt caataagcac atgggattcg caaattttct cagatttata aaccaggact
      781 cgcgaaaaat tccaatctct ttgttaaaaa catgttgagc tttgatctta gaatgatgtg
      841 tccgttttat caatattttg ttggggatag atgggttaga gcgggataat ttatcgttct
      901 tgacagttgt tatttta
//
LOCUS       DBNCWK010000276          868 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.277, whole genome shotgun sequence.
ACCESSION   DBNCWK010000276 DBNCWK010000000
VERSION     DBNCWK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.277"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..>868
                     /gene="legK3"
                     /locus_tag="AC06IO_002929"
     CDS             <1..>868
                     /gene="legK3"
                     /locus_tag="AC06IO_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegK3"
                     /protein_id="HIO9876083.1"
                     /translation="FEFCRKAGHLSIKPPVYNNNKCYLTMKRMPGIDLNRIFNKLMAN
                     PHYLTEGQRIKITYALLLALKNQVIDKGIIHRDIKPENIHVKVVDPVEVNIFDYGLAK
                     NAGEDDNLFPGSESWAAPEMFTGLNHDSKADIFSMGRVIAFLWGESATNYLSNNPLNK
                     SRNAAKNLNLPAQVKWVEPFLKQMLAERQEDRIEIDPAINQFKQLASINAPKLKLPQS
                     SIKNHSNINNLCFFQNPTKSTNNSDLIQLNIQRDIKKDLQELLENLPSEFSHYRSNII
                     FKNTIGRFTTIQK"
BASE COUNT          320 a          146 c          152 g          250 t
ORIGIN      
        1 tttgagtttt gtcgaaaagc agggcactta agtataaaac cacctgttta caataataat
       61 aaatgttatc tgacgatgaa acgaatgcca gggatagatt taaatcgtat cttcaataaa
      121 ttaatggcta atcctcatta tcttacagag ggtcagcgaa ttaagataac ctatgcttta
      181 ttattggctt tgaaaaatca ggtgattgat aaaggaatta ttcatcgaga tataaagcct
      241 gagaatattc atgttaaagt ggttgaccct gttgaagtga atattttcga ttacggactt
      301 gccaaaaatg caggcgaaga cgataaccta tttcctggaa gtgaaagttg ggcagcgcct
      361 gaaatgttta ctggcttaaa tcacgatagt aaagctgata ttttttcaat gggaagagtg
      421 attgccttct tatggggcga aagtgctaca aactatttga gcaataaccc ccttaataaa
      481 agccgaaatg cagcaaaaaa ccttaatttg cccgcacaag ttaaatgggt agagccattt
      541 ttgaaacaaa tgttagcgga aagacaagag gatagaatag agattgaccc cgcaataaac
      601 caatttaagc aacttgcgtc cattaacgcg ccgaagctta aattacctca atcctcaata
      661 aagaatcact caaatattaa taatctctgc ttttttcaaa atccaactaa aagcacaaac
      721 aattctgatc ttatacaatt aaatattcag cgagatatca aaaaggattt gcaggaatta
      781 ttagagaatc ttcctagtga attttctcat tatcgcagta atataatttt taaaaacaca
      841 ataggccgtt ttacaacaat acaaaaaa
//
LOCUS       DBNCWK010000277          862 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.278, whole genome shotgun sequence.
ACCESSION   DBNCWK010000277 DBNCWK010000000
VERSION     DBNCWK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.278"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            75..800
                     /locus_tag="AC06IO_002930"
     CDS             75..800
                     /locus_tag="AC06IO_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0172 family Dot/Icm T4SS effector"
                     /protein_id="HIO9876084.1"
                     /translation="MLYNKWGYLMTTLYIKNLRYNEVAVCRALFEAAEKTEEGIKTKR
                     YGMQMTADMPGLWSPLSNYDIENDLRMARVHRNGRMREIGYLTVNLVFQFDSLDVTEY
                     ETKYGISAKDVLEKAGFTCSQDAAGYSEPASTSTRLLGQESKPEKPTQTSVVNTSFFA
                     LKSVDTDKRFTKSTKPGILYEARSYGKIEDDTLKSYGFTEGQWERQKEGIENIVIIKK
                     LTQSISPSPAVLDEAVDNIPTLS"
BASE COUNT          307 a          137 c          175 g          243 t
ORIGIN      
        1 ggagtaatta atgtgttttc attaagtcca atcagtaaac acgcttttat tttgagcaaa
       61 aatggataaa atgcctgctt tacaataaat ggggttatct aatgacaacg ctttacataa
      121 aaaatttaag atataatgaa gtagcagtct gtcgtgcctt atttgaagcg gctgaaaaga
      181 ctgaagaagg aataaagacc aagagatatg gcatgcaaat gacggctgat atgcctggtt
      241 tatggtctcc tctttctaat tatgacattg agaatgatct cagaatggca agagttcatc
      301 gcaatggaag aatgagggaa attggatatt tgacagttaa tcttgtattt caatttgata
      361 gcctcgatgt tacagagtat gaaacaaaat atggcatttc agcaaaagac gttttagaaa
      421 aagctggctt tacatgcagt caagatgctg cgggttattc tgagcctgct tcaacttcaa
      481 caaggttatt aggccaagaa agcaaacccg aaaaaccaac ccaaacaagt gttgttaata
      541 cctcattttt tgctctaaag agtgtggata cagataaaag atttaccaag tcgacaaaac
      601 caggaatatt atacgaggca agatcctacg gtaaaataga agatgatact ttgaaaagct
      661 atggttttac tgaaggacaa tgggaaaggc agaaagaggg tatcgagaat atcgttatta
      721 ttaaaaaact cactcagagt atttctccaa gtccagccgt tttggatgaa gctgttgaca
      781 atattccaac cctatcctaa ggggtatcaa ttttaaaata ccgaaattga aaaataatgg
      841 ctcactgtca atcaatttcg gt
//
LOCUS       DBNCWK010000278          848 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.279, whole genome shotgun sequence.
ACCESSION   DBNCWK010000278 DBNCWK010000000
VERSION     DBNCWK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.279"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..688)
                     /locus_tag="AC06IO_002931"
     CDS             complement(<1..688)
                     /locus_tag="AC06IO_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HIO9876085.1"
                     /translation="MLVSSIWFIKDRLQTLMLVQDIRRFLDELRESKTILPCDKRLSA
                     ILQEHFSHRDSHEELTSNDIQIVLQCFSERWIADSECDYLLYPSQANQVWIKLAHEIE
                     PFTDKNYLQILLPHITNQFDFNNLTPLTETVRLENFYLGYDGKTLYRKRGLCERLLDN
                     QFELSTCRTLKTKQCEVMTIEELTRLYKGKYCNGEFSIDKEKFDNFWDFLYKKAFPRM
                     QSRGEIPLEVL"
BASE COUNT          292 a          147 c          130 g          279 t
ORIGIN      
        1 gaagtacttc taatggaatt tcacctcttg attgcattcg gggaaaagct tttttgtata
       61 gaaaatccca aaaattatca aatttttctt tgtcaataga aaattctcca ttgcaatatt
      121 ttcctttata taacctcgtt aattcttcaa tagtcattac ttcacattgt ttagttttta
      181 gtgttctgca agtagataac tcaaattgat tatcaagcaa acgttcacat aatcctcttt
      241 ttctatataa agtttttcca tcatatccaa gataaaaatt ttccaatcga acggtttcag
      301 tcaatggagt taaattatta aaatcaaact gattggttat gtggggcagc aaaatttgca
      361 aataattttt atctgtgaat ggttctatct catgagccaa tttgatccaa acctggtttg
      421 cctgagaagg ataaagcaga taatcacact ctgagtcagc aatccagcgt tcgctaaaac
      481 attgcaaaac aatttgaata tcattggagg ttaactcttc atgactgtct ctatgggaga
      541 aatgttcttg taaaatagcg gataatcttt tatcacaggg aagaatagtt ttcgattccc
      601 tcagctcatc aagaaaccta cgaatgtctt gtactaacat taaagtctgc agcctatcct
      661 taataaacca aattgaacta acaagcattc tctatcaata ctattgaact ggaaccacct
      721 aaacaatgtc acgcttgcta ataagtagga attattttga tatcagattg tactttggtc
      781 aatagaggat ttaattaata gaaaaaaaca tgtgaaaaaa aagaagcggc ctaaaaggcc
      841 gctataag
//
LOCUS       DBNCWK010000279          820 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.280, whole genome shotgun sequence.
ACCESSION   DBNCWK010000279 DBNCWK010000000
VERSION     DBNCWK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.280"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            47..241
                     /locus_tag="AC06IO_002932"
     CDS             47..241
                     /locus_tag="AC06IO_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876086.1"
                     /translation="MNRLAMIAAASCLALFLTGCGEGSKPADTKTDQQQQQQPTDQQQ
                     QGGENTGNNQAQPSTEGQGQ"
     gene            318..812
                     /locus_tag="AC06IO_002933"
     CDS             318..812
                     /locus_tag="AC06IO_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIO9876087.1"
                     /translation="MGHFSILVEEIAAYLKKAKWQLVTAESCTGGLIAACLTEIPGSS
                     NWFERGFVTYSNLAKEEMLAVAHELIQTHGAVSEEVALAMAVGALQHSMGNIAVSVTG
                     IAGPEGGSIEKPVGTVCFAWAVHHQFQQTSRQQFTGSRQEIRLSACQYALNGVLSTLK
                     KVCG"
BASE COUNT          261 a          138 c          186 g          235 t
ORIGIN      
        1 gctagaatta acgtgatatt acaaaaaata aatagggaga gtgattatga atcgtttagc
       61 tatgattgct gctgctagtt gtctagcatt atttttaact ggatgtggtg agggctcgaa
      121 gcctgcagat acaaagactg atcaacaaca gcaacaacaa ccaactgatc agcaacaaca
      181 aggtggtgaa aatactggaa ataaccaagc ccaaccttct actgaaggcc aaggccagta
      241 agattaattt cagtgttatt tataaattat caaaaaaccc catagatgtt ttatggggtt
      301 ttttataggt gaaaggtatg ggtcatttct caatactggt tgaagaaata gctgcctatt
      361 taaaaaaagc aaaatggcaa ttagtgactg cggaatcttg tacaggaggt ttaattgctg
      421 cttgccttac ggaaatacct ggaagctcaa attggtttga gcgtggtttt gttacttata
      481 gtaatttagc taaagaggaa atgcttgctg ttgctcacga gctaatacaa actcatgggg
      541 cagtcagtga agaagtggct ttggctatgg ctgttggtgc gttacaacat agtatgggta
      601 atatcgctgt atcagttact ggcatcgcag gtccagaagg aggcagtatt gaaaaaccag
      661 taggtactgt atgctttgcc tgggcagttc accatcaatt tcaacaaaca tcaagacaac
      721 aattcactgg aagtcggcaa gaaatacgtt tgtctgcctg tcaatatgct ttaaatggag
      781 ttctttctac tcttaaaaaa gtctgtggct aaccgaatgg
//
LOCUS       DBNCWK010000280          811 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.281, whole genome shotgun sequence.
ACCESSION   DBNCWK010000280 DBNCWK010000000
VERSION     DBNCWK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.281"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..>811
                     /locus_tag="AC06IO_002934"
     CDS             <1..>811
                     /locus_tag="AC06IO_002934"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIO9876088.1"
                     /translation="EHHLKEKSLALLLIDLDNFKIVNDTLGHPTGDKLLMAVTNRMTR
                     CLRANDFIARLGGDEFIVLLDQCNTDTAVKVAEKIINVLGKPFQFDEYHMTITASIGI
                     AIYPETSEIASDLLKYADIAMYQSKKTGKNKCTVFSKNLHYKENQRNEILKKDIELAA
                     LKRELYIDYQPQIDLSDNTIVGIEALLRWSHPTLGLILPTEFIPIAEQTGCIHTISQW
                     FIEQTLLDYSQFQQLSSNIKLSINVSLSQLNDTRFTKILCDSLLQNNLSKSK"
BASE COUNT          299 a          151 c          137 g          224 t
ORIGIN      
        1 tgagcatcat ctgaaagaaa aaagcctggc attattatta atcgatttag ataactttaa
       61 aattgttaat gatacactag ggcatccaac aggagacaaa ctactgatgg ctgttaccaa
      121 tagaatgacc cgatgcctaa gagcaaatga ttttattgcc cgccttggag gagatgagtt
      181 catcgtcctt cttgatcaat gcaataccga taccgcagtt aaggttgcag aaaagattat
      241 taacgtgctc ggcaaacctt tccaatttga tgaatatcat atgacaatca cagccagtat
      301 tggcattgcc atctatcctg aaacaagtga aatagccagc gacttactaa agtatgccga
      361 tattgcaatg taccaatcca aaaaaacagg taaaaacaag tgcactgtgt tttcaaaaaa
      421 cttacactat aaggaaaatc aaagaaatga aattctcaaa aaagacatag aattagcagc
      481 attaaaaaga gagctctata tagactacca accccaaatt gacttatccg ataacacaat
      541 agttggaatt gaagctttat tacgatggtc acaccctact ctgggtttaa tattacctac
      601 tgagtttatt cctatagcag agcaaacagg gtgtatacat accatcagtc aatggttcat
      661 agaacagaca ttacttgatt actcccagtt tcagcaatta agttcaaaca tcaaattgtc
      721 gataaatgtt tccttgagtc aattaaatga cacgcgattt actaaaatat tgtgtgatag
      781 tttattgcaa aataatttaa gcaagagcaa g
//
LOCUS       DBNCWK010000281          807 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.282, whole genome shotgun sequence.
ACCESSION   DBNCWK010000281 DBNCWK010000000
VERSION     DBNCWK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.282"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            92..>807
                     /locus_tag="AC06IO_002935"
     CDS             92..>807
                     /locus_tag="AC06IO_002935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIO9876089.1"
                     /translation="MKINDFRYMRRGKHLSELQSEEANLLAPVNQRGKGTERALLVLH
                     GFSSSPAVYRYLIPNLKNYDAILCPVLAGHAESIEAFSKSKAADWLQSAKNACELLIN
                     QYQKVDVLGLSLGGLLACQLSQSYAINHLFLLAPALKLHMRINAMLKLAQILKCLGFR
                     QLRNAAGNIITDEHAEIAYRKLPISTIIEMLTLAQEYEWLPPTCPADLFLGIHDQVVA
                     SQQVEQLFCNLPNVKIHWLK"
BASE COUNT          250 a          179 c          140 g          238 t
ORIGIN      
        1 tacactttct ttttggaaag cctattcacc ccaaattaaa caagttacta tagcaaccat
       61 atatggcatg acaatgaaaa atgaatattc tatgaaaatc aatgattttc ggtatatgcg
      121 ccgcggcaaa catctcagcg aactgcagtc ggaggaagca aatctccttg ctccagtcaa
      181 tcagcgtggt aaaggcacag aacgagcttt actggtattg catggtttct cttcttcacc
      241 ggcagtttac cgatatctga ttcctaattt aaaaaattat gatgccatcc tttgtcctgt
      301 attggctggt cacgccgaaa gtatagaagc cttttcaaaa tccaaagcag ctgattggct
      361 tcaatcagca aaaaacgcct gtgaactctt aataaatcag tatcaaaaag ttgatgtttt
      421 aggattatcc ctgggaggat tattagcctg tcaattaagt caatcctatg ctatcaatca
      481 cctgtttcta ttagcccccg cattaaaatt acacatgcgc atcaacgcca tgctgaaatt
      541 agctcagatt cttaagtgcc tcggatttcg acaacttcgc aacgctgcag ggaatattat
      601 caccgatgag catgccgaaa tcgcttatag aaaacttccc atttccacaa tcattgaaat
      661 gctaaccttg gctcaggaat atgaatggct ccccccaact tgcccggccg atctttttct
      721 tggaattcat gatcaagtgg ttgcttctca acaagtagaa caattatttt gtaatttacc
      781 taatgtaaaa atacattggt taaaaaa
//
LOCUS       DBNCWK010000282          754 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.283, whole genome shotgun sequence.
ACCESSION   DBNCWK010000282 DBNCWK010000000
VERSION     DBNCWK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.283"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(164..730)
                     /gene="lirB"
                     /locus_tag="AC06IO_002936"
     CDS             complement(164..730)
                     /gene="lirB"
                     /locus_tag="AC06IO_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system effector LirB"
                     /protein_id="HIO9876090.1"
                     /translation="MNKIICLLSNLLLAVGIMSAHAETAIIKTSEGNITCELFTKEAP
                     NTVANFVGLATGTKEFKDIKTGKMVKRPFYNGLNFHRVIAGFMIQGGDPLGNGTGGPG
                     YTFDNENTNASFNKPGVLAMANAGPNTNGSQFFITVAPTPELQGNYNVFGQVISGQEV
                     VDKISKMPTDPQDKPITPVVIENITIQR"
BASE COUNT          220 a          141 c          143 g          250 t
ORIGIN      
        1 tttggagcta cagataataa agttgatgga ggaaatgcta atacttgtaa ccacatcact
       61 ccagagaata aataaaagtg ttttgttgga attggtcgta tagatgcggc caatttatta
      121 aattgaatta gtttcggctt aatctgacac atgcactgat cttttatctt tgaatggtaa
      181 tgttttcaat gactacaggc gttattggtt tatcttgcgg atcagtaggc atcttgctaa
      241 tcttatcaac aacttcttgt ccagaaatta cttgaccaaa aacattgtag ttgccctgaa
      301 gttcaggtgt tggcgcaacg gtaataaaaa attgactgcc attcgtattg ggtcctgcat
      361 tagccatggc aagtacgcca ggtttgttaa aactggcatt agtattttca ttatcaaatg
      421 tgtaacctgg cccaccggtc ccatttccaa gcgggtctcc gccttgaatc ataaaaccag
      481 caatcacacg atgaaagttt aagccattgt aaaaagggcg tttaaccatt ttgccagttt
      541 ttatgtcttt aaattctttt gttccagttg ccagccctac aaaatttgca acggtattgg
      601 gagcttcttt tgtaaataat tcacacgtta tgttgccctc tgaagtttta ataattgcag
      661 tttctgcatg cgccgacatg atgcctactg ctaacaataa gttgctgagt aaacaaataa
      721 ttttattcat aataagttcc tcatagaatg gcta
//
LOCUS       DBNCWK010000283          735 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.284, whole genome shotgun sequence.
ACCESSION   DBNCWK010000283 DBNCWK010000000
VERSION     DBNCWK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.284"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..563)
                     /locus_tag="AC06IO_002937"
     CDS             complement(<1..563)
                     /locus_tag="AC06IO_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876091.1"
                     /translation="MFIFKRWKIRKITKRIKAMQANRISNQPGDEVLKKEILYYFELA
                     TIFKKLKNHKKYPYAEVMMIECYRAAANLDDSAANFQLGQIFLDEAKYRQKLDNEGIF
                     NSQANLKRAQQLFDEAHAHLIAAEKLGHVGAKRLRGLCIINGWGVESDKNAGFELVVD
                     SIEQEGSWDKIPQIFASMGLNKPEFFSA"
BASE COUNT          202 a          150 c          108 g          275 t
ORIGIN      
        1 gctgagaaaa attctggctt atttaatccc attgatgcaa atatttgagg tattttatcc
       61 caacttccct cttgttcaat actgtcgaca accaattcga atccagcatt cttatcgctt
      121 tcaacacccc agccgttgat aatacataat cctcttaatc tcttggcacc tacatgtccc
      181 agtttttcag ctgcgattaa atgtgcatgg gcctcatcaa aaagttgctg tgctcttttt
      241 aagttggctt gactattaaa tattccttca ttatccagtt tttgcctgta ttttgcttcg
      301 tccagaaaaa tttggcctag ctgaaaatta gctgccgagt catcgagatt agctgctgct
      361 ctataacact caatcatcat aacttctgcg taggggtatt ttttatgatt ctttaatttt
      421 ttaaaaattg tggctaattc aaaataataa aggatttctt ttttaagaac ttcatctcca
      481 ggttgattac ttattctatt ggcttgcata gccttgattc tttttgtgat ttttcttatt
      541 ttccatcgtt taaagataaa cataccctac tccctcacct aagctgttag gtcaaataat
      601 atttgtagta aaaaaattat tgcaacaatt aaaaccatta ggagaatgat tttcaatccc
      661 aatatagtcg aatctctccc acgacctgca ggtttggaag gtgatttttc ttgcatactt
      721 tctaaaggtt tttcc
//
LOCUS       DBNCWK010000284          701 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.285, whole genome shotgun sequence.
ACCESSION   DBNCWK010000284 DBNCWK010000000
VERSION     DBNCWK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.285"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..>701
                     /locus_tag="AC06IO_002938"
     CDS             <1..>701
                     /locus_tag="AC06IO_002938"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014074.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDH family Clp fold serine proteinase"
                     /protein_id="HIO9876092.1"
                     /translation="NIYRLIKNERIDFIKQKIIEYTLTKLPENNYFYLFLYKPNESIN
                     NLDSDRIYWAISDCMGKDILLTINSNGGQIEPAYFISTICKEKSNNKFIVSIPSRAKS
                     AATLIALGADEIHMGSLSELGPIDPQFKGLPALSLSSALECICELCKKYPEASNMFAE
                     YLSKTLTLEQLGYFERISGSAEHYAIRLLENKIDTLTNNPGKISHRLVYEYKDHAFVI
                     DKDEAKEIFGPGVVK"
BASE COUNT          260 a          107 c          120 g          214 t
ORIGIN      
        1 caatatttat cgattaatta aaaatgaaag aatagatttc ataaaacaaa aaatcattga
       61 atatacctta actaagctgc ctgaaaataa ttatttttat ctcttcctgt ataaacctaa
      121 tgaatcaata aataatttag actctgatag gatctattgg gcaatttcag attgtatggg
      181 caaagatata ctactcacaa ttaatagtaa tgggggtcaa attgaacctg cttattttat
      241 ttcaacaata tgcaaggaaa aatcaaacaa taaatttatc gtatccatac caagcagggc
      301 taaatctgca gcaaccttga ttgcgctagg tgcagatgaa attcacatgg gctcattaag
      361 cgaattaggg cctatagatc ctcagttcaa ggggcttcct gcattaagtt taagtagtgc
      421 tttagagtgt atatgtgagc tttgtaaaaa atatccagaa gcatcaaaca tgttcgcgga
      481 gtatctgtct aagaccctta ctttggaaca actgggttat tttgaaagaa tttcaggctc
      541 agccgaacat tatgcaattc gattattaga aaacaaaata gatactctaa ctaataatcc
      601 aggaaaaatt tcacatagat tagtctatga atataaagat catgcttttg taatagataa
      661 agatgaagcc aaagagatat ttggtcctgg agttgtgaaa a
//
LOCUS       DBNCWK010000285          686 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.286, whole genome shotgun sequence.
ACCESSION   DBNCWK010000285 DBNCWK010000000
VERSION     DBNCWK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.286"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..679)
                     /locus_tag="AC06IO_002939"
     CDS             complement(<1..679)
                     /locus_tag="AC06IO_002939"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIO9876093.1"
                     /translation="MKRFFSILFILYCCNLFAMAPKLNWDKAIDKAIKRYGLRVEPQL
                     ISYFSKAGVSYPPKEIALLAFKSERKVELWAKNPSQEWTHIHDYPLTGFSGRLGPKLR
                     ENDKQIPEGIYKLVNFNPFSSMHLSMMINYPNSFDRQKGYMDGRKELGNNIFIHGKNL
                     SVGCLAVGDLAIDQLFILARRVGLKNIQVIIAPNDLRYQKPSTSTFAQPRWLPELYKQ
                     ITESLKPF"
BASE COUNT          210 a          131 c          127 g          218 t
ORIGIN      
        1 taaagggttt gagtgattct gttatttgct tataaagctc gggcaaccat ctgggttgag
       61 caaatgtaga tgttgatggc ttttgatatc gtaagtcatt aggggcaata ataacctgaa
      121 tatttttgag gccgactctt cttgccaaga taaacagttg atcaatggca aggtcaccga
      181 cagccaaaca tccgacagat aaattttttc catgaataaa aatattattc cctaactctt
      241 tcctaccgtc catataacct ttttgccgat cgaaactatt tgggtagtta atcatcatgg
      301 ataagtgcat gctgctaaaa ggattaaaat ttactaattt atagatccct tcggggatct
      361 gtttatcatt ctcacgtaat ttgggtccta aacgcccgct aaaaccagtt aatgggtaat
      421 catgaatatg cgtccattcc tggcttggat tttttgccca taactcaact ttacgctctg
      481 acttgaaggc aagcaaggca atttctttag gagggtagct aaccccggct ttagaaaaat
      541 aagaaattaa ttgcggttct actcttagtc cataacgttt tattgctttg tcaatcgctt
      601 tatcccagtt aagcttaggt gccattgcaa ataaattaca gcagtaaaga ataaacaata
      661 tcgaaaaaaa tcgtttcata gacgca
//
LOCUS       DBNCWK010000286          664 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.287, whole genome shotgun sequence.
ACCESSION   DBNCWK010000286 DBNCWK010000000
VERSION     DBNCWK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.287"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..>664)
                     /locus_tag="AC06IO_002940"
     CDS             complement(<1..>664)
                     /locus_tag="AC06IO_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIO9876094.1"
                     /translation="GVGPNSNSPHSFATGIYSEGNLCSIESNTISLGYATGNGENVGI
                     ALYGGIDCRIKNNHIIFARWPMWGRNFGIWIKSSRSGHPVIENNFIRGAHYALGPFGL
                     FSGNIAVNLSCAFFVQRKNKANQFFDLGKNVAVMQSSVNEGMGGNSICPDNRVYAAYR
                     FIVNPGAFSAYSAAMAWGEGGEETLIETYAWFLVASYYKHELALNIVNNPFANGYSEN
                     VVS"
BASE COUNT          226 a          145 c           96 g          197 t
ORIGIN      
        1 ttgagactac attttctgaa taaccattag cgaatggatt gtttactatg tttaaagcaa
       61 gctcatgttt gtagtaagaa gcaactaaaa accatgcata agtttcaatt aatgtttctt
      121 ctcccccttc tccccaagcc attgcggcag aataagctga aaatgctcca ggatttacta
      181 taaatcgata tgcagcataa actctgttat caggacaaat actgttaccc cccattcctt
      241 cgtttacaga agattgcata actgcaacat tcttacccaa atcaaaaaat tgatttgctt
      301 tattttttct ttgaacaaaa aaagcacatg acaagttaac agctatattt ccactaaata
      361 gtccgaaggg accaagagca taatgtgcac ccctgataaa gttattttca ataacaggat
      421 gaccacttct ggacgacttg atccaaattc caaaatttcg tccccacatt ggccaacgcg
      481 caaaaataat atgattattc tttatacgac aatctattcc accatataaa gcaattccaa
      541 cattttctcc attacctgta gcatatccta gacttatggt attagactca attgaacaga
      601 gattaccttc agaataaatc cctgtagcaa aagaatgtgg tgaattagag ttagggccca
      661 cacc
//
LOCUS       DBNCWK010000287          658 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.288, whole genome shotgun sequence.
ACCESSION   DBNCWK010000287 DBNCWK010000000
VERSION     DBNCWK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.288"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(109..639)
                     /locus_tag="AC06IO_002941"
     CDS             complement(109..639)
                     /locus_tag="AC06IO_002941"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIO9876095.1"
                     /translation="MITIRLAEMNEIQFLNQLISCSARELSQEDYTKEEIEAAIQYVF
                     GVDLELILDKTYFVVERDGQIAGCGGWSRRRTLFGGSQYAGREQGIYLDPQQDFAKIR
                     AFFIHPKFARQGLGSILLKHCEQEALLQKFTRLEMMATLPGVKLYSAFGYKPISNEVI
                     TLPNNVPLRFVRMTKG"
BASE COUNT          210 a          145 c           95 g          208 t
ORIGIN      
        1 atcggattgt attcattaag ataaccgttc ctaacattat gatgataaat taagtttcaa
       61 taaccatgac tcaataatga ctccagacag tataaaacga attacgatct agcctttcgt
      121 catccgaaca aatcttaacg gaacattatt gggaagagta atcacttcat ttgaaatagg
      181 cttatagcca aaagcgctgt agagcttaac cccaggtaaa gtggccatca tctcaagccg
      241 agtaaatttt tgtaaaagag cctcttgttc acaatgcttc aacagaatac ttcccaatcc
      301 ttgtcttgca aatttgggat gaataaaaaa agctcttatt tttgcaaaat cttgttgagg
      361 atccagatat atcccctgtt ctctacctgc atattgactg ccgccaaata atgttcttct
      421 acgactccat ccaccacaac cggcaatttg cccatccctt tctacgacaa aatacgtttt
      481 gtctaaaatt aactctaaat cgacaccaaa tacatattga atggctgctt caatttcttc
      541 ctttgtataa tcttcctggc taagctctct tgctgaacaa gaaattaatt gatttaaaaa
      601 ttgaatctcg ttcatttcag ctaatctgat tgtaatcatt ggtaacctcc tgcaagtc
//
LOCUS       DBNCWK010000288          657 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.289, whole genome shotgun sequence.
ACCESSION   DBNCWK010000288 DBNCWK010000000
VERSION     DBNCWK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.289"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            298..513
                     /locus_tag="AC06IO_002942"
     CDS             298..513
                     /locus_tag="AC06IO_002942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876096.1"
                     /translation="MKKTFLAVLMFSGLNGGVYASAFCNGFEEGYKTIKGDMVIVPIC
                     PIEPITPIGSNSYREGIKAGIRAANED"
BASE COUNT          215 a           93 c          114 g          235 t
ORIGIN      
        1 aagtaaatta acaaatctca tatcatatgg taccagtaac gaattcattt tttcggattt
       61 tgaatctttt gctagaaaaa tattagaact tgttgttcct gacttaataa gatcttatta
      121 ataaagtaag caatcatttt attacctata taacgtaatg tggttgcttc tcttagactg
      181 cattagtact tattatgtat agcagggatc tatttgtatg gataatattt gatcaactgt
      241 ccacaaacaa atataatgcc tttcaatttt tagaacgaga agcgataagg tagaaatatg
      301 aaaaaaactt ttttagccgt actgatgttt tcaggactaa atggcggtgt ttatgcttca
      361 gcattttgta atggttttga ggaggggtat aagactataa aaggagatat ggttatagtc
      421 ccaatttgtc ccatagaacc aattacacca attggaagta actcttatag agaaggcata
      481 aaagcaggta ttagagctgc gaatgaggac taagtaccaa attccctcta tttacggaaa
      541 aatgtattta tttgaggatt aggggcacca atatctctat gagcagttat tctcataatt
      601 tttgcttata tagatggtaa tccccccatt tttttatttg cttttgattt atctatt
//
LOCUS       DBNCWK010000289          648 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.290, whole genome shotgun sequence.
ACCESSION   DBNCWK010000289 DBNCWK010000000
VERSION     DBNCWK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.290"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(80..589)
                     /locus_tag="AC06IO_002943"
     CDS             complement(80..589)
                     /locus_tag="AC06IO_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876097.1"
                     /translation="MKKWNSFLLLIYLSLFIGHAHAVTSLSTNASLLTNFAQLVKAVE
                     QGDDVKAIIRLDRCQITDPALQTQLVQNLDGASTRFNFTEYLHFRTRINDQLRDTVTT
                     VSSKIFEYSPGVFLSVSGRLNVFEDNTATLHVNFYNPALGTSVLIIDWLCDISNGNDE
                     NGLFLYNSP"
BASE COUNT          239 a          120 c          102 g          187 t
ORIGIN      
        1 tcttaagaag actaggtgaa tatgaacaat cattaagaag ctagcaaaat gaaaggggta
       61 aattttatac cttcaggcct taaggactat tgtataaaaa caaaccattt tcatcattgc
      121 cattactgat atcacaaagc caatcgataa ttaacacact tgtacctaaa gccggattat
      181 agaaattaac atgcaaagta gcggtattat cttcgaaaac attcaaacgc ccggaaacag
      241 ataaaaagac acccggcgaa tattcaaata tcttgcttga gacagtagtt accgtatctc
      301 gtaattgatc attaattctg gttcgaaaat gcaaatactc tgtaaaattg aatcttgttg
      361 aagcaccatc caaattctgt actaattgag tttgcagtgc gggatctgta atttggcatc
      421 gatccaatcg tataatggcc ttgacatcat caccttgttc aactgcttta actaattgcg
      481 caaaattagt caacaaggaa gcgttagttg acaaagaagt aactgcatgg gcatggccta
      541 taaacaaaga caaataaatt aacaaaagaa agctattcca tttcttcata acgtcccctc
      601 ttattgattc attatttgct taatctataa gaacaaatat tacataaa
//
LOCUS       DBNCWK010000290          614 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.291, whole genome shotgun sequence.
ACCESSION   DBNCWK010000290 DBNCWK010000000
VERSION     DBNCWK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.291"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            22..>614
                     /locus_tag="AC06IO_002944"
     CDS             22..>614
                     /locus_tag="AC06IO_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system protein LssZ"
                     /protein_id="HIO9876098.1"
                     /translation="MHILAKVIHCIFPLIALTLLIIGIKRNAIYYIISSLWISLISLV
                     IHYQTSGGEILGTYFNYLNAAIYTTNLFVLCLSLVCVLWHLSNDNVAFKIVSSLFQSF
                     IVIGALLVVINLWINAYFIENRLTGTPVMQVALLEKPEYCDYRYIFYKVALDGTVHYL
                     CPNHYGLIPSIGRLSISPDFIASQLSIPSKKQMLLLQ"
BASE COUNT          177 a           97 c          108 g          232 t
ORIGIN      
        1 ataaaataag ggatggaatc aatgcatatc ttggctaaag ttattcactg tatctttcca
       61 ttaattgccc taacactttt aattattgga atcaaacgaa acgcgattta ttatattatt
      121 tcttcgttat ggataagcct tatttcatta gtcattcatt atcagacttc cggtggagag
      181 attttaggta cttattttaa ttatcttaac gcagcaatat ataccaccaa tttgttcgtt
      241 ttgtgcttat ctttggtttg tgttttgtgg catttaagta atgataatgt agcgtttaaa
      301 attgttagca gtttgtttca atcatttata gtcattgggg ctttattggt tgtaatcaat
      361 ctttggatta acgcttattt tatcgaaaac aggttgacag gaacccctgt gatgcaggta
      421 gctcttttag agaaaccgga atattgtgat tacagatata tattttataa ggtggcactg
      481 gatggtactg ttcactatct ttgccctaat cattacggtt taatcccatc gattggccga
      541 ttgtctataa gtccagattt tatagcatcg cagttatcaa taccaagcaa aaagcaaatg
      601 ctcttattgc aaca
//
LOCUS       DBNCWK010000291          611 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.292, whole genome shotgun sequence.
ACCESSION   DBNCWK010000291 DBNCWK010000000
VERSION     DBNCWK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.292"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(123..>611)
                     /locus_tag="AC06IO_002945"
     CDS             complement(123..>611)
                     /locus_tag="AC06IO_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIO9876099.1"
                     /translation="IPYKFAFGMTMCAVSFLMLYFARYFYVGNGMVSSWWMIFSYLFQ
                     SLGELLVSALGVAMVAELVPAANAGFVMGMWFLTSAVAGFIGATVASFTALPENIKPG
                     VESLLIYSDVFACIGLVTLLIAVIMWLVSPRLSRYITVPSLEGDKDIETVEYSTTYIP
                     SH"
BASE COUNT          240 a          133 c          101 g          137 t
ORIGIN      
        1 gtaataaata atttctggtt atcttctatt caagaatgca atagcaatat aggacaaaaa
       61 taaaaaacgg caattggtga tattaaaacc aactcaattg ccgaaaaggg aaataaacaa
      121 acctagtgac tggggatata ggtcgtagag tattctactg tttctatatc cttatcccct
      181 tccaaggaag ggactgtaat atagcggctt agccgaggtg atactaacca cataataact
      241 gcaatcaata gagtaacaag tccaatacag gcaaatacat cggaataaat caataaagac
      301 tcaacccctg gtttgatatt ctctggaagt gctgtaaaag acgcgacagt agcaccaata
      361 aaccccgcca ctgcggaagt taaaaaccac attcccataa caaaccctgc attagcagcc
      421 ggcaccaatt cagcaaccat tgcaacacca agagctgata ctaataactc accaaggctt
      481 tggaataaat aactgaaaat catccaccag gaagaaacca tgccatttcc aacataaaaa
      541 taacgagcaa aatacagcat taaaaagctc actgcacaca tggtcatacc gaatgcaaat
      601 ttataaggta t
//
LOCUS       DBNCWK010000292          609 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.293, whole genome shotgun sequence.
ACCESSION   DBNCWK010000292 DBNCWK010000000
VERSION     DBNCWK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.293"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..592)
                     /gene="regK3"
                     /locus_tag="AC06IO_002946"
     CDS             complement(<1..592)
                     /gene="regK3"
                     /locus_tag="AC06IO_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator RegK3"
                     /protein_id="HIO9876100.1"
                     /translation="MIPEKLLTYTKTIFEYSHSTVYIKDKNFHYQYMNNRGLKQLQLQ
                     EKDIMGMPDKALPFGKYAHFYNSHDYSAMEEIIYFQLDDCITKNGEKIFALSHKLPLK
                     DENGKTHGVIGFTKFMNLHQLMSNLNSKNTFLTPNLKINVNVDDWLKTTINLSRREME
                     VLHYFLQGKSIKLIAPLLHITERTVIFHLNNIKSKWG"
BASE COUNT          199 a           92 c           93 g          225 t
ORIGIN      
        1 aaccccattt gctttttata ttattaagat gaaaaataac ggttctttca gtaatatgaa
       61 gcaatggtgc aataagcttg atcgattttc cttgtaaaaa ataatgcaaa acttccattt
      121 ctctccgaga caaattaatc gttgttttta gccaatcatc aacattaaca ttgattttaa
      181 gattaggtgt taaaaaggta ttttttgaat taaggttact catcagttga tggagattca
      241 taaatttcgt aaagccaatg acaccatgag tttttccatt ttcatcttta agcggtaatt
      301 tgtgagacaa ggcaaatatt ttttcaccgt ttttagtaat acaatcatcc aattgaaagt
      361 aaataatttc ttccattgcc gagtaatcat gactattata aaaatgcgca tatttcccaa
      421 agggaagtgc tttatcaggc atacccataa tatctttttc ttgtaattgc aattgtttga
      481 gtcccctatt attcatatac tggtaatgaa aattcttatc tttgatgtat acggtactat
      541 gggagtattc aaaaattgtt tttgtataag tcaagagttt ttcagggatc ataaagctat
      601 tgtgacaca
//
LOCUS       DBNCWK010000293          593 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.294, whole genome shotgun sequence.
ACCESSION   DBNCWK010000293 DBNCWK010000000
VERSION     DBNCWK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.294"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..314)
                     /locus_tag="AC06IO_002947"
     CDS             complement(<1..314)
                     /locus_tag="AC06IO_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876101.1"
                     /translation="MKNPSILQLNNPALLNKAMQLKSIGKVAISQNQLTYLKIHDEWI
                     HELFPLLQNEQIRKPDYFGNESIGAHISIIYPEENTTINPKELDQEHRFKIKNIIKAN
                     LE"
     gene            complement(413..>593)
                     /locus_tag="AC06IO_002948"
     CDS             complement(413..>593)
                     /locus_tag="AC06IO_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBU_0585 family protein"
                     /protein_id="HIO9876102.1"
                     /translation="TDDINKAYVSPYDKFLYEFDATHKKSASQLQEIKKHERIFKMRD
                     DKDYKIDQSEIWEEF"
BASE COUNT          173 a           96 c           95 g          229 t
ORIGIN      
        1 aattctaaat ttgccttaat aatattctta attttgaagc gatgctcttg atctaactct
       61 tttggattaa ttgttgtatt ttcttctgga tagataatgc taatatgagc accgatggat
      121 tcattaccaa aataatcagg tttacggatc tgctcatttt gtaagagtgg aaatagttcg
      181 tggatccatt catcatgaat cttaagataa gttaattgat tttgagaaat ggctactttc
      241 ccaattgatt taagctgcat agccttattt aataaagcag gattatttag ttgcagaatg
      301 gatggatttt tcatctttat ctttactaat acaataaaga cctgtcattt ctgctactag
      361 gtcttgatta acatgctagc agcttaccaa gatagctata ggtgagaaac tcttaaaact
      421 cttcccaaat ttcactttga tcgatcttgt agtctttatc gtctctcatt ttgaagatgc
      481 gctcatgctt tttaatttct tgcaattgcg atgctgactt tttatgtgtc gcatcaaatt
      541 catataaaaa cttatcgtaa ggactgacat aagctttatt tatatcatct gtg
//
LOCUS       DBNCWK010000294          593 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.295, whole genome shotgun sequence.
ACCESSION   DBNCWK010000294 DBNCWK010000000
VERSION     DBNCWK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.295"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..>593)
                     /locus_tag="AC06IO_002949"
     CDS             complement(<1..>593)
                     /locus_tag="AC06IO_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIO9876103.1"
                     /translation="DFGLFLFENTYLIAFFLFFAVIWAINHFNFMDGLDGFCASQAIF
                     LLAAYALFLNSAGSLIYQDFCLILISSLAGFLIFNFPPAKIFMGDVGSASLGLITFFI
                     AIIAQKNYQIPILYWFILNSLFLFDSTVTLIRRIIKHEKWFSPHKKHAYQRLKQLGIN
                     SRFILLGQMLLNCSFLSIILLHRIIGFNLNYLMLLQL"
BASE COUNT          251 a           93 c           89 g          160 t
ORIGIN      
        1 tagctggagc aacatgagat aattgagatt aaaaccaatt atccgatgca atagaataat
       61 tgataaaaaa ctgcaattta ataacatttg acctaataaa ataaacctcg agttaatgcc
      121 caattgcttt aatctctgat aagcgtgctt tttatgggga ctaaaccatt tttcatgctt
      181 tataatcctt cgaattaaag tcactgtaga atcgaaaaga aataatgaat tcaggataaa
      241 ccaatataga attggaattt gatagttctt ttgagcaatg attgctatga aaaaagtaat
      301 taatcctaag gaggcgctac ctacatctcc cataaatatc ttggctggtg ggaaattaaa
      361 aattaaaaac cctgctaaac tagaaataag aatcaggcaa aaatcttgat aaattaatga
      421 acctgctgag tttaaaaaca acgcatatgc agctaacaaa aaaatcgctt gggaagcgca
      481 aaaaccatca agcccatcca tgaaattaaa atgattaatt gcccaaataa ctgcaaaaaa
      541 tagaaaaaaa gcaattaaat aggtgttttc aaatagaaat aaaccaaaat cta
//
LOCUS       DBNCWK010000295          560 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.296, whole genome shotgun sequence.
ACCESSION   DBNCWK010000295 DBNCWK010000000
VERSION     DBNCWK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.296"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..426)
                     /locus_tag="AC06IO_002950"
     CDS             complement(<1..426)
                     /locus_tag="AC06IO_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876104.1"
                     /translation="MYFSRCISHGAIAGIIAGIVFTFFLVMGGMLETLGSMINMPTKG
                     GGLLVHAVMSIGSGIAFALVLDWLMHSWVSAIFLGLLFGFAMWVGGPMTVLPYLASGV
                     PLFAKWTMEGFKQNIPPLVGHLVYGLILGVTYYAFKRRNK"
BASE COUNT          201 a          140 c           81 g          138 t
ORIGIN      
        1 cttatttctg cgcttgaaag cataataagt aacacccaat atcaacccat acacaagatg
       61 cccaaccaaa gggggtatgt tttgtttaaa tccttccata gtccattttg caaacaaagg
      121 cacccctgaa gccaaatagg gtaacacggt cattgggccg ccgacccaca ttgcaaaacc
      181 gaacaataac ccaagaaaaa tggctgagac ccaggaatgc attaaccaat ccagaaccag
      241 ggcaaaagcg atcccgctgc caatgctcat tacagcatgc accaaaaggc ctcctccttt
      301 tgttggcata ttaatcatac ttcccaatgt ttcgagcatg ccccccataa ctaaaaaaaa
      361 ggtaaaaaca attcctgcta taattcctgc aatagcgcca tgcgaaatac atcttgaaaa
      421 atacatgcca atctccttat taaatagatc ccagtttacc attcaaaatc atcagtttac
      481 ttaataatca acaattaagg tagaaaaatt aatggcaatg tcttaaaaaa tttttgccag
      541 ccacattatc aacatcttat
//
LOCUS       DBNCWK010000296          534 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.297, whole genome shotgun sequence.
ACCESSION   DBNCWK010000296 DBNCWK010000000
VERSION     DBNCWK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.297"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            19..492
                     /locus_tag="AC06IO_002951"
     CDS             19..492
                     /locus_tag="AC06IO_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIO9876105.1"
                     /translation="MMLDRTDKKILEILQANCEINNQELADLVALSPSSCLRRVKLLI
                     DNGYIKKQVALLEPEKVGLKLTVIVLVGLNNHQPEVMNQFEKTIQLFPEVIQCYLITG
                     QSADYLLKIIVPDLNAYQAFLLGKLTRINGVASVHSSFILRNVSDTTALPLDHLD"
BASE COUNT          169 a          101 c           90 g          174 t
ORIGIN      
        1 atttttattg cataaaatat gatgctcgat cgtactgata aaaaaatact tgaaatactg
       61 caagcaaatt gcgaaatcaa taaccaggaa ttggccgacc ttgtagcctt gtctccttct
      121 tcctgtttgc gtcgagtcaa attattaata gataatggtt atatcaagaa acaggttgcc
      181 ctgcttgagc cagaaaaagt agggttaaaa ttgactgtta ttgttttagt agggttaaac
      241 aatcaccagc ctgaggttat gaatcaattt gaaaaaacca tacaactttt tcccgaagtc
      301 attcaatgct acttgattac aggccaatct gctgattatt tattgaagat aattgtgccc
      361 gatttaaacg cttatcaagc ttttttatta ggaaaattaa cacgcatcaa tggcgttgca
      421 agtgttcact ccagctttat attacgcaat gtttctgaca cgaccgccct accccttgat
      481 catttggatt agggcactgt tcttcgaata ttacattatt caataaatac taac
//
LOCUS       DBNCWK010000297          526 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.298, whole genome shotgun sequence.
ACCESSION   DBNCWK010000297 DBNCWK010000000
VERSION     DBNCWK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.298"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..465
                     /locus_tag="AC06IO_002952"
     CDS             <1..465
                     /locus_tag="AC06IO_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009374 - biotin binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="avidin/streptavidin family protein"
                     /protein_id="HIO9876106.1"
                     /translation="LVLSMNIIQELSMKIKWLCSSVFIVSSLVSLPNYAQQDVLTFKN
                     TRGSILELKTLSDNKVEGYFTTAVASKSCPQAIGQKRPITGYAVGNTVTFSVVYPGCG
                     SVLSFSGNFTKKGNIIDTISILTHQAQDITHEGPGARFIGHDRFKKIAASNA"
BASE COUNT          157 a           92 c          102 g          175 t
ORIGIN      
        1 ttggtgcttt caatgaacat tattcaggag ttatcaatga aaataaaatg gttgtgtagt
       61 agtgttttta ttgtctcttc gttggttagt ttgcctaatt acgcccaaca agacgtttta
      121 acttttaaaa acactcgcgg ttcaatttta gaactgaaaa ctctatcaga taataaagtc
      181 gaaggttatt ttaccacggc tgttgcttct aaatcttgtc cccaggcaat tggccaaaaa
      241 agaccaatta cgggctatgc tgtaggtaac actgtgacat ttagtgttgt ttatcccggg
      301 tgtggttctg tattaagttt tagtggtaat ttcactaaaa aaggaaacat cattgatacc
      361 atttccatct tgacccatca ggctcaggat attactcatg aaggaccggg agctcgtttc
      421 ataggacatg atcgttttaa aaaaatagcc gcgtctaatg catagaatgc attttctata
      481 caagcttaca ataagcctca tgttaagaca tgaggcttta atggtt
//
LOCUS       DBNCWK010000298          525 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.299, whole genome shotgun sequence.
ACCESSION   DBNCWK010000298 DBNCWK010000000
VERSION     DBNCWK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.299"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..453
                     /locus_tag="AC06IO_002953"
     CDS             <1..453
                     /locus_tag="AC06IO_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIO9876107.1"
                     /translation="HIDKHHRRMVLEKIIATSIDKYGEEIIEDILFGFEAFKLLQNLA
                     DTDLAKQIQFSDQLDESYFPTNYTLLAENLTFNETELEITSAHLHADDELFQVTRGEL
                     IFYPNSLSPITLKANHRIVIPKGRLHSTVSQSANTQYVVQPIEISTGA"
BASE COUNT          193 a           80 c           84 g          168 t
ORIGIN      
        1 catatagata aacatcatcg acgtatggtt cttgaaaaga taatagctac cagtatcgat
       61 aaatatgggg aagaaatcat cgaggatatt ttatttggat ttgaggcatt caaattactt
      121 caaaacctag cagatactga tttagcaaag caaattcaat tcagtgatca actagatgaa
      181 agctattttc ctactaatta cacacttctt gcggagaatt taacatttaa tgaaactgaa
      241 cttgaaatta cttctgcaca tttacatgca gatgatgaat tatttcaggt cactaggggg
      301 gagttaattt tctatcccaa tagcctaagt cctattacat taaaggctaa tcatagaatt
      361 gtaataccta aagggcggtt acattccact gtttcgcaat ctgcaaatac tcaatatgta
      421 gttcagccaa ttgaaatttc cacaggagca taaaaatgat taataaaaaa tatatcttaa
      481 ataataggta tttttgtaag aacgatgaaa attattttat tgtga
//
LOCUS       DBNCWK010000299          446 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.300, whole genome shotgun sequence.
ACCESSION   DBNCWK010000299 DBNCWK010000000
VERSION     DBNCWK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.300"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            82..>446
                     /locus_tag="AC06IO_002954"
     CDS             82..>446
                     /locus_tag="AC06IO_002954"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIO9876108.1"
                     /translation="MKINATQYVQQCVKPLQRIEAQSEDRFLGIDLLRCLLALGVLLW
                     HYQFFAHNSGIQFIKHQQPLYSILDMFYSHGWQYRVQTFWCISGFILFWKYGEGIANK
                     LISGKKFFIGRFSRIYPLH"
BASE COUNT          142 a           66 c           84 g          154 t
ORIGIN      
        1 tttaaaaaat aatgaaatca ctttgtttta agtgtatttt attgcagctt aaagtattaa
       61 aaaacgcagt gtttagtctg aatgaaaatt aatgctacgc aatatgttca acaatgcgta
      121 aaacctctac agagaataga agctcaatcg gaagatagat tcctcggaat tgatctgctg
      181 cgatgtcttt tagcgctggg tgtgttactc tggcattatc aattttttgc tcataatagt
      241 ggtatacaat ttattaaaca tcaacaacca ctgtattcaa ttttagatat gttctacagc
      301 catgggtggc aataccgggt ccaaaccttt tggtgcatta gtggttttat attattttgg
      361 aaatatggcg agggtatagc aaataagtta ataagtggta aaaagttctt tatagggcga
      421 ttctcaagaa tttacccgct tcattt
//
LOCUS       DBNCWK010000300          417 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.301, whole genome shotgun sequence.
ACCESSION   DBNCWK010000300 DBNCWK010000000
VERSION     DBNCWK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.301"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
BASE COUNT          129 a           66 c           79 g          143 t
ORIGIN      
        1 tatgaataaa atattgttaa agttgggact gccatgtgta tgacacccac ctggtttaac
       61 cgttttaacc gttgtgattg ctccagacaa tcagtaatac attgctggga ttcatacaag
      121 ttttcaactt gtgcagatga ctcttgaaaa atcgattttt gtggtggatc cattaacctg
      181 catacttaaa aacacaattg gtgttttctg agtacaatct cattgtgaga ttttgacagt
      241 tggttaaagc aggaaatttt cgggtgtaaa tttctttcta ccatggtgaa atttgtatat
      301 aaaatacgga aacaggcctg gctcttccct gaagaaaaac cgtatctaaa atcaatttca
      361 actttgatgc ttaggttggt ttattataga tatttaaaga ggtctgctta aataaac
//
LOCUS       DBNCWK010000301          346 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.302, whole genome shotgun sequence.
ACCESSION   DBNCWK010000301 DBNCWK010000000
VERSION     DBNCWK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.302"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..>346
                     /locus_tag="AC06IO_002955"
     rRNA            <1..>346
                     /locus_tag="AC06IO_002955"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a           74 c          102 g           73 t
ORIGIN      
        1 ccataactct gaggtgcgaa gtggttctga ccttttggta cagagaagtc attgatgccc
       61 ggcttccagg aaaagctgct agccataact tatagagaac cgtaccgcaa accgacacag
      121 gtggacaggt agagaatact aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
      181 tggtaccgta acttcgggag aaggtacgcc ctttctggtg atgggattta ctttcagagc
      241 tggagagggc cgcagagacc aggtggctgc gactgtttat taaaaacaca gcactctgca
      301 aattcgtaag aagacgtata gggtgtgacg cctgcccggt gccgga
//
LOCUS       DBNCWK010000302          343 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.denovo.303, whole genome shotgun sequence.
ACCESSION   DBNCWK010000302 DBNCWK010000000
VERSION     DBNCWK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.denovo.303"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            <1..297
                     /locus_tag="AC06IO_002956"
     CDS             <1..297
                     /locus_tag="AC06IO_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HIO9876109.1"
                     /translation="LLMVFIALTMIPYLGNLLIPVMIYFCVLMLMVFSAFQVKKETLT
                     IGSGALFFLISDLTLALNLFIYTQADVRIFVMFTYYVAQFLLTFGLVRLYEKRG"
BASE COUNT           96 a           43 c           62 g          142 t
ORIGIN      
        1 ctattgatgg tatttatagc attgaccatg attccatatc ttggcaattt attaattcca
       61 gtaatgattt atttctgtgt cttgatgctg atggtattta gtgcttttca ggtaaaaaag
      121 gaaactttaa cgattggcag tggagccttg ttctttttga tttctgattt aactttggca
      181 cttaacttgt ttatttatac tcaagctgat gtaaggattt ttgttatgtt tacttattat
      241 gtcgctcaat tcttattaac ttttggtctc gtcagattat atgaaaaaag gggttgatga
      301 atttattatt cagataacat aaaaagggtt catcaagtca aag
//
LOCUS       DBNCWK010000303         5763 bp    DNA     linear   BCT 30-MAR-2026
DEFINITION  TPA_asm: Legionella pneumophila isolate FL-BPHL-00013-2019_LEG
            SAMN56685170-rid29320313.guided.159, whole genome shotgun sequence.
ACCESSION   DBNCWK010000303 DBNCWK010000000
VERSION     DBNCWK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN56685170
            Sequence Read Archive: SRR37747483
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Legionellales; Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-MAR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003154479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003154479.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/24/2026 19:40:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,963
            CDSs (total)                      :: 2,915
            Genes (coding)                    :: 2,891
            CDSs (with protein)               :: 2,891
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 11 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 5 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN56685170-rid29320313.guided.159"
                     /serotype="1"
                     /isolate="FL-BPHL-00013-2019_LEG"
                     /isolation_source="water"
                     /db_xref="taxon:446"
                     /geo_loc_name="USA: Florida"
                     /collection_date="2019"
                     /collected_by="Florida Bureau of Public Health
                     Laboratories"
     gene            complement(<1..594)
                     /locus_tag="AC06IO_002957"
     CDS             complement(<1..594)
                     /locus_tag="AC06IO_002957"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIO9876110.1"
                     /translation="MVEKNKDKVYQVYDEIVDWFDDNRTKDLTMEKLYLDFIQKCLKP
                     KSKILDVGCGTGEPIAQFLINEGYDVTGIDASRKMISLCKQRFPKNKWILADMRTLDL
                     NEKFHAVIAWHSFFHLPHDDQRMTLKLLASYVDQNGLLIFTSGPEYDEVWSNNGGHDL
                     YHASLSTEEYEQILIDNNFKVLAHKIRDPDCGDATVWV"
     gene            708..1700
                     /gene="aph(9)-Ia"
                     /locus_tag="AC06IO_002958"
     CDS             708..1700
                     /gene="aph(9)-Ia"
                     /locus_tag="AC06IO_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HIO9876111.1"
                     /translation="MLRNNITDQHLIALLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     ESNSYFVKLKYGHHDEINLSIIRLLHDSGIKEIIFPIYTRDTKLFQQIDHFKIIVYPF
                     INAPNGFTQNLTEKQWHQLGKVLRQIHETSVPTAIQQRLRKETYSPKWREMVRSFYNK
                     IGFDDSDDQITTDFKTFFNQKIDSIHRLVDSSEELSKKIQLDLDKYVLCHSDVHAGNV
                     LVVNEGSIFIIDWDEPMLAPKERDLMFIGGGIGNVWNKPHEIDYFYEGYGKTNVDKII
                     LSYYRHERIVEDIAVYGQDLLSRDQNNESRLESFKHFKSMFDPNDVVEIAFSSD"
     gene            complement(1703..2116)
                     /locus_tag="AC06IO_002959"
     CDS             complement(1703..2116)
                     /locus_tag="AC06IO_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecDF P1 head subdomain-containing protein"
                     /protein_id="HIO9876112.1"
                     /translation="MIKVIAALVGVFFLPSAMASSPTTTDIDASKDISSLVFQVIQNQ
                     MVLDSSTVESATIVLPETPTDGYGVRIKLKTIAATKFGQMTENGIGKQGSFILNGIVI
                     SNPIIRSRIGDEFIVTGFTKKQANHFIESMSSKNN"
     gene            complement(2189..2485)
                     /locus_tag="AC06IO_002960"
     CDS             complement(2189..2485)
                     /locus_tag="AC06IO_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIO9876113.1"
                     /translation="MTKNKFKATHPLLSLMNVGEATYKDLQLLGINSIHQLANASADQ
                     LYARLQQITGQLHDPCVWDVFAAAINEARTGEKQPWWQWTKIRKKRQLEGTFCI"
     gene            complement(2586..3881)
                     /locus_tag="AC06IO_002961"
     CDS             complement(2586..3881)
                     /locus_tag="AC06IO_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIO9876114.1"
                     /translation="MCALYYNLNNHYASHFVYSPEKIMKKPLDNTEQNRIAALYELKI
                     LDTPADERFDRIIRLTSGYLSVPIAFISFIDSKHQWFKSSCGIGLNKISRNDSFCHYT
                     IQQNKPMIISDALLDERFCNNPYVTNSPNIRFYAGFPLSTVDGFNVGTLCAIDKKPRN
                     LDETEIQILKDLATLAEDQVNLLDISLLERAVREKNISLTKAKKELEMRNNFIRKVFG
                     SFMSDEIVAALLESKSELKLGGEERKITILFSDLRNFTTLSEHFPADKIVAALNNHYG
                     KMVDVIEKYNGTVDSFIGDAIMVVFGAPHSGGDDAYRAIACALEMQATMQEVNTMNRN
                     SSLPELAMGIGINTGYAIVGNIGSQKRMQYSAIGSPVNLASRIQDLTLGGQILISEAT
                     YQEVNRSLELNGHLRVKVKGVAAPITIYDVIGLNKKSDA"
     gene            complement(4047..5075)
                     /locus_tag="AC06IO_002962"
     CDS             complement(4047..5075)
                     /locus_tag="AC06IO_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876115.1"
                     /translation="MRRSKAMQIPKKRTQILTISESSKEDLDVVPEYSEEMDVSPSKE
                     TSKTNSKKLESIARKEQIKKLEGELYSHIQDAIELEEESFAEFSINTGRPSNYLFRFV
                     SEKEYLAILENLNQGKRIFGNKKKVLEEEDEKISTKAKGETFFAFDLKYAQKYFNKGT
                     RRNGVMLAIELHEDADSKLLSNTEIVRRIGDDPGMAGGRWAKCKKVKPGERGVVVAKC
                     EGVRAGCKYPYTLGFREGEEGVSPFDTLFELIRSVKVLDNNGSCKTIYHCSVPAPKEK
                     ELTNIVKDAESGTDGIHSSEFLSVVSSSPSSRFGFFTNNELSGISGLVNKAPEHIIPP
                     SDNTFSHH"
     gene            complement(5285..>5763)
                     /locus_tag="AC06IO_002963"
     CDS             complement(5285..>5763)
                     /locus_tag="AC06IO_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIO9876116.1"
                     /translation="LEEEQRIKLEEEHKSKKYFAQSDAIDLILNNFENEISSIGAYYA
                     PAKQRAIELLDTLRKYKEEAFNDPSREKLISFAQSTKRAIQEATPILQKDLGWGDYLT
                     NLAKQLVNAVTFAVAYAVTFGTTGHQGFFALKSSLAVSQSQNLEDALNRELSQKNC"
BASE COUNT         1767 a         1099 c          912 g         1985 t
ORIGIN      
        1 aacccaaact gtcgcgtctc cacaatctgg atctctaatt ttatgcgcca gtaccttaaa
       61 attattatct ataagtatct gttcatattc ttcggtagaa agagatgcat ggtataaatc
      121 gtgtcctcca ttattactcc aaacttcatc gtattcaggt cctgatgtaa agattaataa
      181 accattttga tcaacatagg aagctagtaa ctttaaagtc attctttgat catcgtgagg
      241 taaatgaaaa aagctatgcc acgcaataac cgcatggaat ttttcattca aatctaaagt
      301 acgcatatct gctaaaatcc atttgttttt tggaaatcgt tgcttacaca agctaatcat
      361 ttttctactg gcatctattc cggttacatc gtaaccttca ttgattaaaa attgggctat
      421 aggttctcca gtaccacaac ctacatctaa tattttgctt ttaggtttga gacatttttg
      481 aataaaatct aaataaagtt tctccatagt caaatcttta gtgcggttat catcaaacca
      541 gtcaacaatc tcgtcatata cctgataaac tttatcttta ttcttttcca ccatgttaaa
      601 ctccagttgc aaacactttt ttatgcgttg aatccaggta ataatagtat tccaattata
      661 ttatttgctt taccattatg gtaatttcca aatagtaatt aactcttatg ctaagaaata
      721 acattacgga tcaacacctc attgcgcttt tgaaagtcta ttatggaatt gatattcata
      781 ctgtacaact catcgtgggt ggtgctgata tgaatgcctt tggatataaa gcggattcag
      841 aatccaattc ttatttcgta aagctgaaat acggccatca tgatgaaatt aatttatcaa
      901 tcattcgcct cttgcatgat tctggaataa aagaaattat ttttcctatc tacacacgtg
      961 atacaaaatt atttcagcaa atagatcatt ttaaaataat agtgtatcca tttattaatg
     1021 cacccaatgg tttcactcaa aatttaacag aaaaacagtg gcaccagctt ggaaaagtgt
     1081 taagacaaat tcatgaaact tctgttccca ccgctattca gcaacgatta aggaaagaaa
     1141 cttattcgcc taaatggcgt gaaatggtta gatcctttta taacaaaatt ggatttgatg
     1201 attcagatga tcaaattacc actgacttca aaactttttt taatcaaaaa atcgattcaa
     1261 ttcatcgatt agtcgattca tcagaagaat tatctaaaaa aattcaactt gatttagata
     1321 aatatgtgct gtgtcattct gatgtacatg cgggcaatgt gttggtcgtt aatgagggat
     1381 ctattttcat tattgattgg gatgagccca tgttagcgcc aaaagagcgt gatttgatgt
     1441 tcattggcgg tggcattggt aatgtatgga ataaacccca tgaaatcgat tatttttatg
     1501 aaggttatgg taaaacaaac gttgataaaa taattttatc ttattacaga catgaacgaa
     1561 ttgtcgaaga tatagctgta tatggacaag acttactttc acgtgatcaa aacaacgagt
     1621 ccagacttga aagttttaaa cattttaaat cgatgtttga tccaaatgat gttgttgaaa
     1681 tagctttttc ttcggattaa ttttaattat tttttgacga catgctttca ataaaatggt
     1741 tggcctgttt cttggtaaac cctgttacaa tgaactcatc ccctatcctg cttcgaatta
     1801 ttggatttga tatcactata ccatttaaga tgaagcttcc ttgttttcct ataccgtttt
     1861 cagtcatttg cccaaacttg gtagctgcaa tagtttttaa tttaatacgt acaccatagc
     1921 cgtctgttgg agtttcaggc aatactattg ttgccgattc aactgttgag ctatccaaaa
     1981 ccatctggtt ttgaatcact tgaaatacca gtgatgaaat atccttactg gcatcaatat
     2041 cagttgttgt gggactagag gccattgctg atggtaaaaa aaatactcca acaagcgcgg
     2101 ctattacttt aatcataata ttcatataag aattattgga aaagagtaaa taggtatatt
     2161 atatcccgtg tttgcaatat tgaatacttt aaatacaaaa tgttccttct agttgtctct
     2221 tttttcgtat ttttgtccat tgccaccaag gttgtttttc acctgtgcgt gcctcgttta
     2281 tagcagcagc aaacacatcc caaacacaag gatcatgtaa ttggccagta atttgttgca
     2341 ggcgtgcgta cagttgatct gcacttgcat tagcaagttg atgaattgaa ttaatcccaa
     2401 gcaattgcaa atccttataa gttgcctccc ccacattcat aagacttaaa agtggatggg
     2461 ttgccttgaa cttatttttg gtcatatcta tcccttaggc tgcttattgc aatcataata
     2521 ccattgaggt tggtaattta agcaatttct tgatcccatg attttaaaga gtgatattat
     2581 tacttctatg catctgattt tttattcaaa ccgattacat cataaatagt gataggagca
     2641 gcaactccct taacttttac tctgagatgc ccatttaact caagcgaacg attaacttcc
     2701 tggtaagttg cctcagaaat taaaatttgc ccccccaaag tcagatcttg gatacgtgat
     2761 gccagattaa ccggagaacc aattgcacta tattgcatac gtttctgaga accaatgtta
     2821 cctacaatag cataacctgt attaattcct atgcccattg ctaattcagg caaactgctg
     2881 ttgcgattca ttgtgttcac ctcttgcatg gtcgcctgca tctctaatgc acaggctatc
     2941 gctctgtatg cgtcatctcc tccagagtgt ggtgctccaa ataccaccat aatggcgtca
     3001 ccaataaagg aatcaacagt accattgtat ttttctatga catcaaccat ttttccataa
     3061 tgattgttaa gtgcggcgac aattttatcc gcaggaaaat gctccgacag agtagtaaaa
     3121 tttcttaagt cgctaaacaa aattgtaatt ttgcgctcct cacctcctaa ttttaactct
     3181 gattttgatt caagaagtgc tgcaacaatc tcgtcagaca taaaactacc aaatacttta
     3241 cgaataaaat tattacgcat ttcaagctct ttcttagctt ttgtcagcga aatgtttttt
     3301 tctcgtacag ctctctcaag aagcgaaata tcaagcagat tgacttgatc ttctgccaaa
     3361 gttgccaaat cttttaaaat ttgaatttca gtctcgtcta aattacgtgg ttttttatct
     3421 attgcgcata gtgttcccac attaaaacca tcaactgtag ataatggaaa tcctgcataa
     3481 aaacgaatat ttggtgaatt agtcacataa ggattattac aaaatcgttc atccaacaag
     3541 gcgtctgaaa taatcatcgg tttattttgc tgaatagtgt agtggcaaaa tgaatcattc
     3601 cgggatattt tgttaagccc tatgccacag ctggatttga accattgatg tttagagtca
     3661 ataaaggaaa taaaagcaat aggaaccgat aagtaacccg aagtgagccg aataattcga
     3721 tcaaatcttt cgtctgctgg tgtatccaga atttttaatt cgtataatgc agcaatgcga
     3781 ttctgctcag tattatccaa aggtttcttc ataattttct caggtgaata aacaaaatga
     3841 gaggcatagt ggttattaag attatagtac aaggcacaca tattaattgg cttaaaccaa
     3901 tgaataagtt aattgtattc agcaatggaa ataaagttga tagcttactt gtatgtggct
     3961 aacccttgct atgaaggata tactcagaaa cgggcaaata aataatattt aattgcccgt
     4021 ctaaagcctt aaaaatttaa cctgatttaa tgatgcgaga aggtattatc agagggcggt
     4081 attatatgtt ccggtgcctt atttaccaat cctgaaatac ctgatagctc attatttgtg
     4141 aagaaaccaa agcgtgatga aggggaactt gaaacaactg ataaaaattc actactatga
     4201 attccatctg taccactttc agcatctttc acaatatttg taagctcttt ttcttttggc
     4261 gcaggtactg agcaatgata aatagtttta caactgccat tgttatcaag gacctttaca
     4321 gaacgtatta gctcaaatag tgtatcgaaa ggactaaccc cctcttctcc ttctctaaac
     4381 cccaaggtat atggatactt acatcctgca cgaactcctt cacatttagc tactactacc
     4441 cctcgttcac caggctttac cttcttgcat ttagcccatc ggccaccagc cattccggga
     4501 tcatctccaa ttcttctaac aatttcagta ttagataata gtttactatc ggcatcttca
     4561 tgcaattcta tggcaagcat tactccatta cgtcgtgtgc ctttattgaa atatttttga
     4621 gcatatttaa gatcaaaagc aaaaaaagtt tctccttttg ctttagttga tatcttttcg
     4681 tcctcttctt ccaatacttt tttcttgtta ccaaatattc tctttccttg atttaaattt
     4741 tcaagaattg ccaaatattc tttttcactg acaaagcgaa ataaataatt actaggtctt
     4801 ccagtgttaa tgctgaattc tgcaaaagat tcttcttcta attcgatagc atcttgaata
     4861 tgactgtaaa gctctccttc taatttcttg atctgctctt tacgagcaat actctccagt
     4921 tttttactgt tagttttgct tgtttctttg ctgggagaaa catccatttc ctcagaatac
     4981 tcgggtacaa catccaaatc ttctttactt gattctgaaa tagttaaaat ttgtgttctt
     5041 tttttaggta tttgcattgc tttacttcta cgcatctaac tctcctaaca tcgttacgat
     5101 atacaatatg tgcttatatt attcttacac gatctatttt gcacattatc agcaaaatga
     5161 aatggattgc aatctttcat ttctaaatca aaattaccga atcgaaatac attagattta
     5221 agaagaatgc ttgggcagca aaaataatgt cttgcccatt agcattctat gtctggtcta
     5281 ttccctaaca gtttttttga ctcaattctc tattgagtgc atcttcaaga ttttgagatt
     5341 gactaactgc cagagaagat ttcaaagcaa aaaagccttg atgcccggta gttccaaaag
     5401 ttacagcata agccacagca aaggtcactg cattaaccaa ttgtttcgct aaattggtta
     5461 aataatctcc ccagcctaaa tctttttgca gtattggtgt tgcttcctga attgctcttt
     5521 ttgtactttg cgcaaaagag attaattttt ctctgcttgg atcattaaat gcttcctctt
     5581 tatattttct caaagtatct agcaattcta tagctctttg ttttgcaggg gcataataag
     5641 caccaataga actaatttca ttttcgaaat tattcaaaat gagatcaata gcatcggact
     5701 gagcaaagta ttttttgctt ttgtgctctt cttcaagttt gattcgttgc tcttcttcaa
     5761 gtt
//
